LOCUS       DAOQGJ010000001        13550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_2110_length_13550_cov_3.377325, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000001 DAOQGJ010000000
VERSION     DAOQGJ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2110_length_13550_cov_3.377325"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK683_00005"
     CDS             <1..382
                     /locus_tag="PK683_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNH06851.1"
                     /translation="RIRMLPASRDENPNAKNSWGADLSQRLKNNMLTYIFQLQFYADE
                     KSTPIEDASVNWEESEIPYLTVAKLHLLKQDTDSPQGKEFSELAEKSVFDPWKALADH
                     RPLGDVMRARKAVYYASQKERGAS"
     gene            406..894
                     /locus_tag="PK683_00010"
     CDS             406..894
                     /locus_tag="PK683_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06852.1"
                     /translation="MEKEIFEAVIGFSRMEEGYVLEYSAAPSSVGKENPIGLSEIKFQ
                     RLSEKKDSIVGSYELTEDVYTDWINKNTERSILSVLADHPEIHLMPEDGFNSLQTEYR
                     HSWSMFFTLTRPGVSKDGNTAIVQITGFCPGGIPNYGSILYMENSGGKWNVLSSFGLY
                     NQ"
     gene            969..1337
                     /locus_tag="PK683_00015"
     CDS             969..1337
                     /locus_tag="PK683_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06853.1"
                     /translation="MSFLKTMPSERILEIRIEPFNTEGSLVKKEIVIRDRKRIEEIAV
                     LIREAEDFRPDHPALKWQAVLRITADREYGGLVLSSSEKHQGVMLTIFSNVIDGWNYG
                     SVRNDRLGDALEKIAETNGN"
     gene            complement(1454..1852)
                     /locus_tag="PK683_00020"
     CDS             complement(1454..1852)
                     /locus_tag="PK683_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06854.1"
                     /translation="MKILSFRTFFLISLVLSSVNLFSDSYREKLLGQWDLKKISDSTQ
                     NIEIPLMSTALWSFREDGTGYIRMEHQGKKEIIEFTWVLNDLVLTVTDKQGKDDKITF
                     GFFEDYMLIMRKPDDVMLLKKKKYPFPEKK"
     gene            1951..2415
                     /locus_tag="PK683_00025"
     CDS             1951..2415
                     /locus_tag="PK683_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06855.1"
                     /translation="MRLFLILQFFSFSLIFSEPLKAGDELKLIQYQTQFGDEVTVPES
                     TSRLIFASEMEASKIAHSVLENAGADYLKNRSAVLVSDIHRMPYFITKFVALPKLKSY
                     SYTIHLIREEGPGEIFPREKGKLTLISLKKYKVLSVKYISSSAELKEELEKK"
     gene            2646..7937
                     /locus_tag="PK683_00030"
     CDS             2646..7937
                     /locus_tag="PK683_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH06856.1"
                     /translation="MFQLPGYDLTSRLHQSRKTFVYKGFRLKDNKPVAVKILRKDIGL
                     NYSGSLKYEFDIIQSIHSSFVIRAIEYFHHSDTSIIVMEETGGESLKNLIHKYKFSLD
                     EKLSIAAKTVKGLIDIHSENIIHKDLNPSNIIYNPENGSLKIIDFGISSQLSKEKAAA
                     ETVNRLEGTLAYISPEQTGRMNRMTDYRTDYYSLGAALYELFSGRTPFESDDPMELVY
                     SHLALIPADLHALIPDFPYTLSNLIMKLLSKNAEARYISGKGILSDIEECQKQLSQSG
                     AVSVFPLGRYDYSGKLVLSEKLYGREKDVSGLLSIFSEISNPYDLLLRKQRGLDFVMV
                     SGTSGIGKSSLVRELQKPIAERKGHFVQGKFGKLTRNVPYKGFISALGELTRHVLSVE
                     ESSEKYKKLFRDALGDNLSIVTEVIPEMEDLLGRHFAEFERDSMEYQTRFKNAILGFL
                     KTFCSGSNPLVIFLDDMQWADIASLQLLAKILEDETIQGLMIICAVRENELDESHPLN
                     MLLAELSRMGISPKKFRLEPLSLESVQDYLMDSLQADREEIKSLSSVFMHKTDGNPFF
                     MNEMLKTIYERGYLKFYFPDTSERSGGKWKWDILEIEKMSVTENVADLLVDKIRKLPS
                     ESQKVLKLAACIGSSFDLKTLSIIYEKDMNYTGAVLWNAMHMELIYPVGEEYKYLQED
                     NLGLPDSIRITYKFTHDKVLQAAYNLLPPESRQSVHLQIGRLLYKYTRTEDLEERLFD
                     IVNHYNLGMEKIESEEENFLLAAVNLGAAKKAMNSVAYNAVIQYCQAGIRCIQEETWT
                     INYKLAYSLHKLRAEAEYLIGHFEKSESYLLALLEKTNSVHDKTEIYSILVSQYNIHA
                     EYSKALSAGRTALKLLGIVVPEKDYGTWLLIENEEIQRYLDQNSIESLIDLPRMTESE
                     KQSAMKLLARVKSTTYLFDIQLYPLIVTVMVKLSLKYGNAPESCFAYSTYGIINATFL
                     NSPEAGYSFAKLAFELSEKFKDKIQKCKTGYILAGYLNFRKEHIKKSFVYFEEAGKAG
                     LESGEIQFTGYNFLFKALISFHSGKKLQEIFAEADAAVSFAMKSNNFWAMDMGRALKL
                     ALASYTGLSGSAEFYHTTEFSEEKFLDESLEHSSKSAVCNLRIYKGIMLFLLLQYSAA
                     RKEFDSAKENSESILGQIEEVLLAFYSGLCSAAVYKETSDLQESGRLLSDFREQMETV
                     KRWADSCSENFQHKYFLMKAEEHSILGENEEASSLYEKSINLAKENGFLHELGVSYEL
                     AGRFYSRRGLKIAARNYLKGAYISYNEWGAFRKTEQLEEEFPNLEGIIQSDTFSMLEG
                     TGSITLSMSASGSLDVLSLFKVTQAISGEIVLGKLLENLVKIMVENSGAEKGYLIMEK
                     KGKLYVENAIVSGHLLSNASTPIESVTALPASVIQYIRRTKESLIIDDAALDIQFSND
                     PYVREKKPRSILCLPLANQGKLLGIVYLENNLMVGAFPKERLQILKIIASQAAISIEN
                     AVLYEHLEEKVAERTKELNDSLRIIKSDLITAKEIQYNILPKQKLNNPAVDYYFEYIP
                     MAEVGGDFIDITEISDSHIRVFLSDATGHGVHASLMTMLIKAEYENLRKYSLQPSLLL
                     ERFNREFCEKYSSLGTFFTCCITDIYFNEKKILYASCGHPNQYLLNSSGLHTMSRTGN
                     LIGLDAENQVHLQELDLETESRIILFTDGIYEEFNPEMEIYGEERLSSFILKNGQESP
                     SSFSKRLISEVKHFTRGKPFQDDVTVLVLNINMDRLK"
     gene            7934..9034
                     /locus_tag="PK683_00035"
     CDS             7934..9034
                     /locus_tag="PK683_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNH06857.1"
                     /translation="MNVYIKNAALSRFGKKDCSVLDLSADTCEKALHGTEREKVDALV
                     ISSFAPEKYTEEYHLGAKLADRLRLKDIFCLRSETASSSGASAVILASNLIQSGRFSN
                     VLVVGTEIMSRLDREKNNILLGSVLSETQRGFAMSMAQGAAMITRLYLKKYNYNEEDL
                     FYIAEKLHLNGSRNPIAHIQKPITREMYNAGPKFATPLGLYDISPLSDGSAALLLSSE
                     KGPLKILGTGYGTAGFSKSDAEPSFSSSISAFSRAFSEAGILPSQIHCAELHDAFTTF
                     ELIGAEDAGLFPRGEALRAVIEGHTHPDSIKPINASGGLKTRGHPIGASGTAQIAELF
                     SFMQKKKLQFGLTHSIGGLATNNFAVILENCG"
     gene            9031..10038
                     /locus_tag="PK683_00040"
     CDS             9031..10038
                     /locus_tag="PK683_00040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNH06858.1"
                     /translation="MKNILIIQTAFLGDLILTTGFIRRVREKNPDSRISILVNKGAED
                     VLAGNPHLNDVIILDKKKIKSSIFGFFKFASEIRARKFDTVLSPHFSHRSSILSFLSG
                     AGSRIGYHEAGFSFLLNRRISRPLHGKHEVEKLFSLIGEENSPYRPELYLPSDRIQNI
                     EKIFKEEIGGRKYIVLAPSSVWETKRMPEDKFRRILELILEKTDYLPVLTGAPKDREL
                     CASIGKGFEAADLTGRTNLVELHYVISKSAGIVSNDSSPIHIASAFNIPIAAVFGATV
                     KDFGYTPLSDKQFIAEISGLKCRPCGIHGGNICPEKHFRCMREQNPENIFEELMKLLS
                     P"
     gene            10114..11190
                     /locus_tag="PK683_00045"
     CDS             10114..11190
                     /locus_tag="PK683_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06859.1"
                     /translation="MVIFWDGSAKKLNGRCLIIALFISRKKKPWKKKGKKNALLSLKQ
                     GIDWIKNESEKYKVKLKIDFICLNMEEDISVKKIPKIEAPYEDMEVIMFKVLKKLGYE
                     SPEDFYEKMNRKYRDTSFHIIFFANEKGRPYMHYVDMNNEKKMLEFNMIFKENGKIDP
                     FTVVHETLHAYSAVDLYNVNETKKGQKISDRVEKRFPTEVMLCASEDIENPELSELTA
                     FLVGWHSDPKDWYASMIQPDDKEEVEFLLKHHESFDEKGFVIIKKEKELLRYESDDFI
                     ISRIEINSNPDLIHWKQISKHSGDTVEYWENSQDSYYYNMTDKKSLLTIAVPKRKKGY
                     CYKEDPDGDYSKTVKVWKTDVSAG"
     gene            11446..12504
                     /locus_tag="PK683_00050"
     CDS             11446..12504
                     /locus_tag="PK683_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06860.1"
                     /translation="MKSFWSGSAKKLQGKCLVVGIFFSEPDKPWTEEGRKNTWKNVKN
                     AMKWIQKNSEDYDIDLEFEYKCMNEDEDVVLDKIPSFEDPMEEGEAAIIKVLEELDFE
                     SPGDFYTNLKEEYEEYSLHVMFFANKDDRAYMGSVDMDNAGKELEYNMIYTEEGVVGS
                     FAVAHEALHAYSAMDLYNVGNTPEGAAAEKRTTKRFPKEVMMTAVDDAEESELSEVTA
                     FLIGWHNKPKHWYTTLMQPGAVEPMAFLLKNHKNFDENGDIRIEDEEELAKYKYKGGE
                     FVRLKINGSDDFIHWKHSDSDSEEETEYWEDSSDDDFYYLTDKKSSKEISVPRETGTC
                     FQLDESGEFQPWIEVKMV"
     gene            complement(12512..>13550)
                     /locus_tag="PK683_00055"
     CDS             complement(12512..>13550)
                     /locus_tag="PK683_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH06861.1"
                     /translation="KDTFITAVTHDLKSPILNTRHILEIMISKEEMDWEERLYYLKTS
                     HHSLTASYQMIYNILNMDRFNSGRAEIHHEYVDLDSLIQKVIQGFEYTLAFKNITLEY
                     LPREEKIVIGDFQLLLQLFSNLISNAVKFSFEKSTVSVSIENIQNFYSIRIKDSGTGM
                     SPEQIKSLSFRFNPVKEGTAGEKGSGLGISIVRQILELHEGSMNIESSLNTGTEISIY
                     LPCQEKVLTVFSEDDSLYNDIDCIKAADEIRILKVYDMNNLEECISRIVPDFIVLGLK
                     NPNSQLMRIIKKIAEREEQPADIFLKIFFERNVIQGKIDSPALNRLFLNDIYADTVKA
                     LTEFLEQKKSR"
BASE COUNT         4113 a         2570 c         3051 g         3816 t
ORIGIN      
        1 ccgtatcaga atgcttccag catccagaga tgaaaatccg aatgcgaaaa acagctgggg
       61 agcggacctg agtcaaagac tgaaaaataa tatgctgact tatatatttc agctccagtt
      121 ttatgcagat gaaaaaagca ccccgattga agatgcctca gtcaactggg aggaatctga
      181 aatcccttat ttgacagttg caaagcttca tctgctgaag caggatacag attctccgca
      241 gggaaaggaa ttttctgaac tggcggaaaa gtctgtcttt gatccatgga aggcacttgc
      301 agatcacaga ccgctcggtg acgtgatgag ggcaagaaag gcagtctact atgcgagtca
      361 gaaggaaaga ggcgcgtcct gagactggga tctgcgggga ttattttgga aaaagaaata
      421 tttgaagcag ttatcggttt cagcagaatg gaggaaggtt atgtactcga atactccgct
      481 gctccttctt ctgtcggaaa agaaaatccg atcggtctca gtgaaataaa attccaaagg
      541 ctgtcggaaa agaaggacag cattgtcgga agttatgaac tgacggaaga tgtatataca
      601 gactggataa ataaaaatac agaaagatcc attctttcag ttcttgcaga tcatcctgaa
      661 atccatctga tgcccgaaga cggcttcaat tctcttcaga ctgaatacag acattcctgg
      721 tcgatgttct ttaccctgac aaggccaggg gtgtcaaaag acgggaatac tgcaattgtg
      781 cagattaccg gcttctgtcc tggaggaatt ccaaattacg ggagcattct gtatatggaa
      841 aacagcggcg ggaaatggaa tgttctctcc tcattcggac tgtacaatca atgaaaagat
      901 atatatgttt atattatatg tttttggttc ttccctggat tctttcctgt tttgggaagg
      961 agaagacagt gtcatttctg aagacgatgc cttctgaaag gattctggaa atcagaattg
     1021 agccgttcaa tacggaaggt tccctggtaa agaaagagat tgtcattcgt gacaggaaaa
     1081 gaattgaaga gattgctgtt ctgatcaggg aagcggagga tttccgtcct gaccaccctg
     1141 cactgaagtg gcaggccgtt ctgagaatta cagctgacag agagtacgga ggactggtgc
     1201 tttcgtcatc agaaaagcat cagggagtga tgcttacgat tttcagcaat gtaattgacg
     1261 gatggaatta cggttcggtg cgaaatgacc ggctgggaga tgctcttgaa aaaattgcag
     1321 agacaaacgg aaactgagct ggtaaatcag tatattatat gcctgtagaa aatctggtgt
     1381 cctgaatgat tcggacacct ctggcgtgta cggtaaggca aggaaggtct gaccttctct
     1441 gaaatcaggc agcttacttc ttttcaggaa aaggatactt cttttttttc agaagcatta
     1501 catcatccgg ttttctcata atcagcatat agtcttcaaa aaaaccgaag gtgattttgt
     1561 cgtcttttcc ctgcttgtca gtaacggtca ggaccagatc attcagaacc catgtgaatt
     1621 caataatttc tttttttccc tgatgctcca ttctgatata accggttccg tcttcacgaa
     1681 aactccaaag agcagttgac ataagcggga tctcaatatt ctgagtgctg tctgaaattt
     1741 ttttaagatc ccattgtccg agaagttttt ctctgtagga gtctgaaaac agatttacgg
     1801 aacttaagac aagggagatt agaaagaatg ttctgaagga tagtattttc attctgcaag
     1861 aatgcccgtt ctgttttcgg aggcaagcag aaactgtgca atttgaataa tcttctttaa
     1921 aattaaaagc ctgagtacag tattgccttt atgagactgt ttctgatttt acaatttttt
     1981 tctttttctc tgattttttc ggagcctctg aaggcgggtg atgaactgaa actgatccag
     2041 tatcagactc agttcggtga tgaggtgact gttcctgaaa gcacatccag gctgattttc
     2101 gcctctgaaa tggaagcctc aaagattgct cattctgtcc ttgaaaatgc aggtgcggat
     2161 tatctgaaaa acagatctgc tgttcttgtt tcggatattc atcggatgcc ttattttatc
     2221 actaaatttg ttgcactgcc taaactgaaa tcttacagtt acacaattca tctgatcaga
     2281 gaagaaggtc cgggtgaaat ttttccgaga gaaaaaggaa agctgactct gatttcatta
     2341 aaaaaatata aagttctttc cgttaaatat atttccagtt ccgctgagct caaggaagaa
     2401 ctcgaaaaaa aatgacagta tatgtctgct gtttaataaa gtgagctgct gactcatatt
     2461 gtgcaaatgg gatattttac agtttttatc agactgtata tttttgaatc ggcagaggcg
     2521 gagttcccgg cagcagctcc gaaaaatcta ccggcacttt gcagtgagct tgccgaatcc
     2581 aggcattctc tgccaatact cacctgctgt ctgaatgcgg cggtgagtca ctaaaggagt
     2641 aatctatgtt tcagcttccc ggatatgatc tgacgtccag acttcatcag agcagaaaaa
     2701 cctttgtata caaaggtttc agactgaagg acaacaagcc tgttgcagtc aaaatactga
     2761 gaaaagacat aggtctcaat tattcaggaa gcctgaagta tgaatttgac atcatccagt
     2821 ccattcattc cagtttcgta atcagggcaa ttgagtattt ccaccattcc gacacttcca
     2881 tcattgtaat ggaagagaca ggcggcgaat cccttaaaaa tctgatccat aaatacaaat
     2941 tctccctgga tgaaaaactg tccattgcag caaagacagt gaaaggtctg attgacattc
     3001 attccgaaaa catcattcat aaggatctga atccttccaa tatcatctat aatcctgaaa
     3061 atggatcatt aaaaattata gactttggga tatcttccca gctttccaag gaaaaggctg
     3121 cagcagagac agtgaacaga ctggaaggga cgcttgcata tatctctccc gagcagacag
     3181 gcagaatgaa caggatgact gattatcgga ctgattacta ttcgctggga gcggctctgt
     3241 atgagctttt ttcaggcaga acgccctttg aatccgacga tcctatggag cttgtgtatt
     3301 ctcaccttgc actgattccc gctgatctgc acgcactgat ccctgatttt ccttatactc
     3361 tttcgaatct tataatgaag cttctgtcca agaacgcaga ggcaaggtac atcagcggaa
     3421 agggaatact gagcgatatt gaagagtgtc agaaacagct gtctcagtca ggcgccgtat
     3481 ccgtatttcc gttaggaaga tatgactatt caggaaaact tgtgctgtct gaaaaactgt
     3541 atggaaggga aaaggatgta tccggtctgc tttccatttt ttctgaaatc agcaaccctt
     3601 acgatctgct gcttcgaaag cagagaggtc ttgattttgt tatggtgagc ggcacatccg
     3661 gaataggaaa atccagtctt gtcagagaac ttcagaagcc cattgcggaa aggaaaggcc
     3721 attttgtaca aggcaagttc ggaaagctga ccagaaatgt tccttacaaa ggatttatat
     3781 ctgcactcgg agaactgact cgtcatgtac tcagcgtaga agagtcttct gagaaataca
     3841 aaaaactttt ccgtgatgca cttggggaca atctttccat tgtgacagaa gtcattcctg
     3901 aaatggagga tcttcttgga agacattttg cagagtttga aagagattca atggagtacc
     3961 agaccagatt caaaaatgca atactgggat ttctgaagac tttctgcagc ggatcaaatc
     4021 cccttgtaat ttttctggat gatatgcagt gggcggatat tgcaagtctt cagctccttg
     4081 ccaagatact tgaagatgag actatccagg gacttatgat tatatgtgct gtcagagaaa
     4141 atgaactcga tgaaagccat cctctgaata tgcttcttgc ggaactttcc cggatgggaa
     4201 tcagtcctaa aaaattcagg ctggaacctc tttctcttga atcggttcag gactatctga
     4261 tggattcact tcaggcagac agggaagaaa tcaaatcgct ctcatcggtg tttatgcaca
     4321 agacggacgg aaatcctttt ttcatgaatg aaatgctcaa gactatatat gaaagaggtt
     4381 atctaaagtt ttactttccg gatacctctg aacgttccgg ggggaaatgg aaatgggata
     4441 ttctggaaat agaaaaaatg tcagtgacgg agaatgtcgc cgacctgctt gtggacaaga
     4501 tcagaaagct cccttctgaa tcacagaaag tcctgaaact ggctgcctgc atcggaagca
     4561 gttttgatct gaagactctc tccatcattt atgaaaagga tatgaattat accggggcag
     4621 ttctgtggaa tgcaatgcat atggagctta tttatccagt gggagaagaa tacaagtatc
     4681 ttcaggaaga caatctgggt ctgcctgatt ccataaggat tacttacaaa tttactcatg
     4741 acaaggtgct tcaggctgca tacaatctat tgcctcctga atccagacag tcagtacatc
     4801 ttcagatagg aagactcctt tacaagtata cgagaacgga agatctcgaa gagaggctct
     4861 ttgacatagt caatcattac aacctgggaa tggagaagat tgaatctgag gaggaaaatt
     4921 ttcttcttgc tgcagtgaat ctgggtgcag ccaaaaaagc aatgaattca gtggcttaca
     4981 atgctgtgat tcagtactgt caggccggca taagatgcat ccaggaagag acttggacga
     5041 taaattataa gcttgcttac agtcttcaca agcttcgtgc agaggctgaa tatctgatag
     5101 gacattttga aaagtcggag agttatcttc tggcccttct ggaaaagaca aactcagtgc
     5161 atgacaagac ggaaatttat tccatactgg tgtctcagta caatattcat gcagaatatt
     5221 ccaaagctct ttcggcagga aggacagcat tgaagcttct cggaattgta gttcctgaaa
     5281 aggactatgg aacctggctc ttgattgaaa atgaagagat acagagatat ctcgatcaga
     5341 acagcattga gagtctgatt gatcttccca ggatgactga gtccgaaaag cagtctgcca
     5401 tgaagcttct tgccagagtg aagtctacga catatctctt tgatatacag ctttaccctc
     5461 tgattgtgac tgtgatggtc aaactttctc tgaaatacgg aaatgctcct gagtcctgtt
     5521 ttgcctattc gacatacgga atcattaacg caacttttct gaacagtccg gaggcaggat
     5581 attcgtttgc aaaacttgca tttgaactgt ctgaaaaatt caaggacaag attcagaaat
     5641 gcaagactgg ctatattctg gccggttatc tgaatttcag aaaagagcac attaaaaagt
     5701 catttgtata ttttgaggaa gccggaaagg caggattgga atcgggagag attcagttta
     5761 caggatacaa ttttcttttc aaggcactga tttcgtttca ttcaggaaag aagcttcagg
     5821 aaatttttgc agaggcagat gctgcagtca gttttgcaat gaagtcaaat aatttctggg
     5881 cgatggatat gggaagggca ttgaagcttg cattggcttc gtacaccggt ctttccggct
     5941 cggcagaatt ttatcatacc actgaatttt cagaagagaa atttcttgat gagtcgcttg
     6001 aacattccag caaaagtgca gtatgcaatc tcagaattta caaaggcatt atgctttttc
     6061 ttctgcttca gtattctgca gcaagaaagg aatttgattc agctaaggaa aattcagagt
     6121 cgattctcgg acagatagag gaggttcttc tggcatttta ttccggactg tgttcagctg
     6181 cagtatacaa ggagacgtcc gatcttcagg agtcgggaag acttctttcc gattttcgtg
     6241 aacagatgga gacagtcaag cgatgggctg actcatgttc agagaatttt cagcataaat
     6301 attttttaat gaaagcggaa gagcactcga ttctgggaga aaatgaagag gcctcatccc
     6361 tgtatgaaaa atcaatcaac cttgcaaaag agaacggttt tctccatgaa ctcggagtat
     6421 cctatgagct tgcaggaaga ttttattcca ggcggggtct caaaattgcc gccagaaact
     6481 atctgaaggg cgcgtatata tcttataatg aatggggagc attcagaaaa acggaacagc
     6541 tcgaggagga gtttcccaat ctggaaggaa ttattcagtc ggatacattt tcaatgcttg
     6601 aagggactgg aagcatcact ctttcaatga gtgcctccgg aagcctggat gttctttcac
     6661 ttttcaaggt gactcaggca atatccggag aaattgttct tggaaaactt ctggaaaatc
     6721 ttgtcaagat aatggtggag aattcaggtg cggaaaaagg atatttaatc atggaaaaaa
     6781 agggaaaatt gtatgtagaa aatgcaattg tatccggaca tcttctttcc aacgcatcaa
     6841 ctccgattga atcagtgacc gctcttccag cgagcgtgat tcagtatatc agaaggacaa
     6901 aggagagtct gatcattgat gatgctgctt tggatattca gttttccaat gacccttatg
     6961 tgagagagaa aaaacccaga tctattctgt gtctgcctct tgcaaatcag ggaaaacttc
     7021 tgggaatagt ctatctggag aacaatctga tggttggagc atttcccaag gaaaggcttc
     7081 agatcctgaa gatcattgct tctcaagcgg ccatttccat cgagaatgca gttctgtatg
     7141 aacatctgga agaaaaggtt gcggaaagaa caaaagaact gaatgacagt ctgcggatca
     7201 tcaagtcgga tctgattact gcaaaggaga ttcagtacaa tattcttcct aagcagaagc
     7261 tgaacaatcc tgcagtggat tattatttcg aatatatccc tatggctgaa gtagggggtg
     7321 actttatcga catcactgaa atatccgaca gccatatcag ggtatttctt tccgatgcta
     7381 ccggtcatgg agttcatgca tctctcatga ctatgctcat caaggctgaa tatgaaaatc
     7441 tgagaaagta ttctcttcag ccctctctgc ttcttgaaag attcaaccgt gaattctgcg
     7501 aaaaatattc cagtctggga acattcttca cctgctgcat aacggatata tattttaatg
     7561 aaaaaaaaat actttatgct tcctgcggtc atcctaatca gtatctgctg aacagttccg
     7621 gtctccatac aatgagccgg acaggaaatc tcatcggact ggatgctgaa aaccaggtcc
     7681 atctgcagga actggatctg gaaacggaga gcaggatcat tctgtttact gacggaattt
     7741 atgaagaatt caatccagag atggaaattt acggagaaga aaggctgagt tccttcattc
     7801 tgaaaaacgg tcaggaaagt ccctcatcct tttccaaaag acttatcagc gaagtgaaac
     7861 attttaccag aggaaaaccc tttcaggacg atgtgactgt tcttgtcctg aatataaata
     7921 tggacagact gaaatgaatg tatatataaa aaatgctgcc ctcagcagat ttgggaaaaa
     7981 ggattgctct gttctggatc tttctgcaga tacctgtgaa aaggcgctgc acggaacgga
     8041 acgggagaag gtggatgcgc ttgtcatatc ttcctttgct ccggaaaaat atacggaaga
     8101 gtatcatctg ggagcaaagc ttgcagacag acttcgtcta aaggatattt tctgccttcg
     8161 ttctgagaca gcctcttcga gcggtgcaag tgcggtcatt cttgcatcga atctgattca
     8221 atccggcaga ttcagcaatg tgcttgttgt gggaaccgaa atcatgagcc gactggacag
     8281 ggaaaagaac aatatacttc tcggttccgt tctctctgaa actcagaggg gtttcgcaat
     8341 gtccatggca cagggtgcag caatgatcac tcgactgtat ctcaaaaagt ataattataa
     8401 tgaagaagat ttattttata ttgcagaaaa acttcatctg aacggaagca ggaatcctat
     8461 agcgcatatt cagaaaccga ttaccagaga gatgtacaat gcaggtccta aatttgcgac
     8521 acctcttgga ctctatgata tttctcctct ttctgacggt tcagccgcac tgcttctttc
     8581 ttcagaaaag ggtcccttga aaattcttgg aactggctat ggaactgcgg gtttctccaa
     8641 atccgatgca gagccttcct tttcgtcttc tatttctgca ttcagccggg ctttttcgga
     8701 agcaggaatc cttccttctc agattcactg tgctgaactg catgatgcct ttaccacatt
     8761 tgaactgatc ggtgcggagg atgcaggact gtttccgcga ggagaggcct tgagggcagt
     8821 catagaggga catactcatc ctgattcgat taagccgatc aacgcttcag gcggactgaa
     8881 gacaagagga catccgatcg gtgccagcgg aacagcccag attgcagaac tgttttcctt
     8941 tatgcagaag aaaaaacttc agtttggtct gactcattca atcgggggac ttgctacgaa
     9001 caattttgcg gtaattctgg agaactgcgg atgaaaaata tactgatcat acagactgct
     9061 tttttgggtg atctgatact gactacaggc tttatcagga gagtgagaga gaagaatcct
     9121 gatagcagaa tttcgatttt ggtcaataaa ggagcggaag atgttctggc tggaaatcct
     9181 catctgaatg atgtaattat tctggataaa aaaaagataa aatcttctat attcggtttt
     9241 ttcaaatttg cttctgaaat cagagcaagg aaatttgata ctgtcttaag tcctcatttt
     9301 tctcaccgtt cgagcattct ttcctttctg tctggagcag gcagcagaat cggatatcat
     9361 gaagcagggt tcagtttttt actgaacaga aggatttcaa ggccgcttca tggaaaacat
     9421 gaggtggaaa agcttttcag cctgattgga gaagagaatt ctccttacag accagaactc
     9481 tatcttccgt cagacaggat acagaatata gaaaaaatat tcaaagaaga gattggcgga
     9541 agaaagtata ttgttctggc cccttcttct gtctgggaaa ccaaaagaat gcctgaggac
     9601 aagtttcgca ggatactgga gctgattctg gaaaagacgg attatcttcc tgttctgacc
     9661 ggagcaccca aggacagaga actgtgtgca tccatcggaa aggggtttga ggcggctgat
     9721 ctgactggaa ggacgaatct ggtggaactg cattatgtta tatctaaatc agcaggaatt
     9781 gtatccaatg attcctctcc gattcatatt gcatctgcat tcaatatacc gattgctgca
     9841 gtcttcggtg ctacagtaaa ggatttcgga tatacacctc tttcagataa acagtttatt
     9901 gctgagattt caggcctgaa gtgcagaccc tgcggaattc acgggggaaa catctgtcct
     9961 gaaaagcatt tcagatgcat gagggaacag aatcctgaaa atatttttga agaactgatg
    10021 aaacttttgt ctccttagtt tacggtgaaa tcggtatgca gataagagaa cacagctcag
    10081 tcaaatttgc gagatgaata gtcaggaacc tgtatggtaa tcttctggga cggatctgca
    10141 aaaaaactca acggccgatg cctgatcatc gcactgttca tttccaggaa gaaaaagccc
    10201 tggaagaaga aaggaaaaaa gaatgccctt ctgagcctga agcagggcat tgactggata
    10261 aagaatgagt cggaaaagta taaagtaaaa ctgaagattg atttcatatg tctgaatatg
    10321 gaagaggata tttcggtcaa aaaaattccg aagattgaag caccttatga agatatggaa
    10381 gtaattatgt tcaaggttct gaaaaaactg ggatacgagt cccccgaaga tttttatgaa
    10441 aaaatgaaca ggaaatacag ggacacaagc tttcatatca tcttttttgc caatgagaag
    10501 ggcagacctt atatgcacta tgtcgatatg aacaatgaaa aaaagatgct cgagtttaat
    10561 atgatcttca aggaaaacgg aaagattgat ccttttactg ttgttcatga aactctgcat
    10621 gcttacagtg cagttgatct gtacaatgtg aatgagacta agaaaggcca gaaaatatcc
    10681 gacagagttg aaaaaagatt tcccacagag gtgatgctct gtgcttctga agacattgaa
    10741 aaccctgagc tttccgagct gactgcattt cttgtgggct ggcacagtga tcctaaggac
    10801 tggtacgcct ccatgataca gcctgatgac aaagaggaag tggagtttct tctgaagcac
    10861 catgaaagct ttgatgaaaa gggatttgtc attataaaaa aggaaaaaga actgctcaga
    10921 tatgaatctg acgatttcat catttcgagg attgaaatta acagcaaccc tgacctgatt
    10981 cactggaaac agatcagtaa gcattccggg gatacagttg aatactggga aaacagtcag
    11041 gacagttatt attataatat gacagataaa aagtctctac ttacaattgc agtgcccaaa
    11101 agaaaaaagg gatactgtta caaggaagat ccggacggtg actacagcaa gacagtaaag
    11161 gtttggaaaa cggatgtttc tgcaggataa gactttgagt tcgaataatt gacacaggat
    11221 gtgcatcaat cagtttgaag atttcagttc ctaaggtaga atccgttttc aataagatag
    11281 tccggacatt ttcagttata agttgaagtt aaatctgcat aacttgttcc atcctctaaa
    11341 ttttgttcag gaaaaaatca gatgtttttc tgcaggacct ctgatctttc cttgacagca
    11401 ttttccatac tgttactctg tcttccaatt tattcaggag catttatgaa atcattttgg
    11461 agcggatctg caaaaaaact gcaagggaaa tgccttgttg tcggaatttt cttttcggag
    11521 cctgacaagc cttggactga ggaaggacgg aagaatacct ggaagaatgt aaagaatgca
    11581 atgaaatgga ttcagaaaaa ttcagaagat tatgatattg atctcgaatt tgaatacaaa
    11641 tgcatgaatg aggatgagga tgttgttctg gataaaatac cttcatttga agatcctatg
    11701 gaggaaggtg aagctgcaat catcaaagtt ctggaagagc ttgattttga atctcctgga
    11761 gatttttata caaacctgaa agaggaatac gaagaataca gtcttcatgt tatgtttttt
    11821 gcaaataagg atgacagggc ttatatgggc agtgtggata tggacaatgc cggaaaagaa
    11881 ctggaataca atatgattta tacagaagag ggagttgtcg gttcttttgc agttgctcat
    11941 gaagcacttc atgcatacag tgcaatggat ttatacaatg taggcaatac tccggaaggt
    12001 gctgctgcag aaaaaaggac aacaaagaga tttccgaaag aagtgatgat gactgcagtt
    12061 gatgatgccg aagaatctga gctttcagaa gtgacagcct ttcttatcgg ctggcataat
    12121 aaacctaagc attggtatac aactcttatg cagccgggag cagttgaacc gatggctttt
    12181 cttctcaaga atcataagaa ctttgatgaa aacggtgata tcaggattga agacgaggag
    12241 gaacttgcaa aatataaata caaaggcggt gagtttgtcc gtctgaaaat caacggaagc
    12301 gatgatttta ttcactggaa acattcggac tccgattcag aagaggagac tgaatactgg
    12361 gaagacagca gtgatgatga tttttattat ctgacggaca agaagtcttc caaggaaatc
    12421 tctgttccaa gggaaaccgg tacatgcttt caactggatg aatcaggtga gtttcagccc
    12481 tggattgaag tgaaaatggt ctaaggacag attaccgact ttttttctgt tccagaaatt
    12541 cagtaagtgc ttttactgta tcagcatata tgtcattcag gaacagcctg ttcagggcag
    12601 gggaatctat ttttccctga atgacatttc gttcaaaaaa tatcttcaga aaaatatctg
    12661 caggctgctc ttctctctcc gcaatttttt ttattattct cataagctga gaatttgggt
    12721 ttttcagtcc aagtacaatg aaatcaggaa caatcctgct gatgcattct tcaagattat
    12781 tcatatcata caccttcaga attcggatct cgtctgcagc ttttatgcag tcaatatcat
    12841 tatagaggga gtcgtcttct gagaaaacag tcagaacttt ttcctggcag ggaagatata
    12901 tgctgatctc tgtgccggtg ttcagacttg actcaatatt cattgagcct tcatgcagtt
    12961 ccagaatctg tctgactatg ctgattccga gaccggatcc tttttctcct gccgttcctt
    13021 ccttgacagg attaaatcgg aaactgagag acttaatctg ctccggactc atccctgttc
    13081 cggaatcctt aattctgata ctgtaaaagt tttggatatt ttcaatagac actgagactg
    13141 ttgacttctc aaatgaaaac ttaacggcat tgctgatcag atttgaaaac agctgaagca
    13201 gaagctggaa atcgccgata acaatttttt cttctctcgg caggtattcc aaagtaatat
    13261 ttttgaatgc caaagtatat tcgaatccct gaatcacttt ctgaatcagg ctgtccagat
    13321 ctacatattc atgatgaatt tctgctcttc ctgaattgaa tctgtccata ttcagtatat
    13381 tatatatcat ctgataagaa gcagtcagtg aatgatgaga agtctttaaa taatacagtc
    13441 tttcttccca gtccatctct tccttgctga tcattatttc cagtatatgc cttgtgttca
    13501 gtatgggaga tttcagatca tgtgtgactg cagtaataaa agtatccttc
//
LOCUS       DAOQGJ010000002         8454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_3984_length_8454_cov_3.292297, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000002 DAOQGJ010000000
VERSION     DAOQGJ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3984_length_8454_cov_3.292297"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="PK683_00060"
     CDS             complement(<1..1333)
                     /locus_tag="PK683_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06862.1"
                     /translation="MEYEEHIQPKTREARLRDKKRIAVCGGSLGREQRSLVRGQLVDI
                     GVPNIAEAADLWDFLTGFFKGEENDVTPFKDLGLAPVRKPILSIELFNEKDKKVFESE
                     NFNGNEDGFFSYEIRKKLRPGKYTFHVIFRGIDSYRQYTQDIAYMNLRDKSDITKSTI
                     VGIGQMRVLPESYNDFITTSDIDQTYLATELGSKKGMLSTLFETPNEKFFLPGMPELY
                     WTLRRDTEDTPLCFISASPHFFRRSLLSTFKHHGVQTESLHLKYLAGTVKGVVDKVVA
                     SITRPDQILKEGIQPAVERVKKFFHSSYLSLFDQMSYKLTILLEDRIYQPSKAKEILL
                     GDNTESDYFIFTLYQLILMGEIEGKRLEDYLYRLNFLGRDAVTRDQAKRIRSLAEENI
                     SIHGNRNPVRAVLINLTHIGPREDRMKQSVLDALPPGVNLDRIKKFVWYHPT"
     gene            complement(1663..1926)
                     /locus_tag="PK683_00065"
     CDS             complement(1663..1926)
                     /locus_tag="PK683_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06863.1"
                     /translation="MKKTVFRTVLAGVIAFFLSSGLSAKSHKGHKPKAKAQVCKCCGM
                     KMEKGKMSKASKSAKLPQCNMKSKNGKWNCGPAKAKKHKKKHH"
     gene            2169..2756
                     /locus_tag="PK683_00070"
     CDS             2169..2756
                     /locus_tag="PK683_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06864.1"
                     /translation="MGFRFVPVKESFDASFYGMLPGFSGIEAYHEASADETAETQIPK
                     SEPKTMSEAISEGMSRASQDSILVNSIVREVTWNIERNSEKYGVLVSSGSSRLYDPYY
                     RKSDPVFDYSVSFKVKQVIHSYKMHSYYIFPFTGFVWIFSSNPMQEIYHRAVLEMTVK
                     DRDGKTVKIKELNVPAYEPLYFWKFNWLKTVDDRR"
     gene            complement(2823..3800)
                     /locus_tag="PK683_00075"
     CDS             complement(2823..3800)
                     /locus_tag="PK683_00075"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNH06865.1"
                     /translation="MSALEKVNSVSGDLTKHFGRYQGLLNFDSSKWDGNKGYLSERRL
                     QRKAWIFSGLITEDFTVGFAIVDAGLIATAFAYIYQRKTGLFLEEKMDKILGFSDEFQ
                     PSLQDAWKLEKGNQVWEFMPVKGGYSILYSGDKISLKYVLQENENGMSTIAPAKDRPF
                     HTTYKNMNLPAEVRIKIGKEEHSWTADCGTVDFSKGYPGRETFWNWASFSGKAETGES
                     FALNLVAGFNEGLENNAWINGEILPLGIAKFSYKKPISQNECEIITMDGLSEFRFIPE
                     GKRSEDINLVLFQSRFVQAYGRFEGNILYNGKTLKIKGFGLVEEHYAVW"
     gene            complement(4010..4423)
                     /locus_tag="PK683_00080"
     CDS             complement(4010..4423)
                     /locus_tag="PK683_00080"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HNH06866.1"
                     /translation="MIVVSNTSPVIAFGKLNRFSILEAVFGKIIIPSYVFLELNNLKY
                     KDEINLKESVFKTEEISFVDPLLEKELDRREAEAITLALKKNADWLIIDERKGRNIAK
                     NVYGINVIGTAGILLKAKESGIINQVSPLLQELKK"
     gene            complement(4420..4668)
                     /locus_tag="PK683_00085"
     CDS             complement(4420..4668)
                     /locus_tag="PK683_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNH06867.1"
                     /translation="MIEVPDSLLLKWDGSKEKIRAEAQKLLAVKLFEEGFLTTGQAAE
                     MCGMNRYDFISDLSRRGIPVVQMNTEELEEEIKNSRKL"
     gene            complement(4780..6282)
                     /locus_tag="PK683_00090"
     CDS             complement(4780..6282)
                     /locus_tag="PK683_00090"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH06868.1"
                     /translation="MTEKGKHLKEVTRGDIMKNTADAVVIGTGFAGLCAAIKLKEKGM
                     NFIVLEKDSGVGGVWRVNTYPGAQCDVQSHLYSFSFEHKSDWSRTYGMQKEILEYMEH
                     CSSKYGINPHIRFNTEAVDAKWNDSEKRWEIRTDKGDIIQAKYAFFGLGGLSQIVLPD
                     IKGREKFKGKFFHSAKWDHSADVKGKKVAVIGSAASAIQIVPAIASKVKKLTVFQRTA
                     SWILPKEDRPYSNIEKNAFQMFPPLQWASREAIYWRLEARAMAFVYTPAVMKAFQRLI
                     EKHIRNCVKDPVLAAKLTPDYTIGCKRILLSNDYYPALQRSNVDLVTEGIAEMDENGI
                     ILKDGSRVDADIIVAATGFKAAEDILPFPVLGKNGISLNDEWKNGPEAYLGTTVKGFP
                     NMFMIVGPNTGLGHSSMVYMIESQVNYAMKAVEYLQEEKLNMLEVKPEIQEEFNKEVQ
                     SRLGNSVWNNGGCVSWYRTKDGKNTTLWPGFTFEFRNRTENFQPKEYILK"
     gene            complement(6295..7263)
                     /locus_tag="PK683_00095"
     CDS             complement(6295..7263)
                     /locus_tag="PK683_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH06869.1"
                     /translation="MIAIEKTFHEMYGLVPDDIRKGIGEFLWNLNHKVLYISTGAKEK
                     TVQLRDVEISYIELGDIRKPTLVIVHGFSDSKEGFLSLAGHLARDFHILIPDLPGFGK
                     SSKPNCTYSLELYSEWLLEFLNYKKVYKFHSLGNSMGGAILIEYAHRHPDSVESLVLS
                     CAAGLVPEEEGNHFYDDYRSGKNIFIIEEEYHFEEFVGKLFYKKNMVPHTVKHYMFQK
                     YRHNGAWYDKLMNDILGKYVGKNEEEHQRIQREKISSIECPVLILWGSHDGIFPEKIA
                     HQFKEQIPHATLQVVDETGHLPHMEKPKEVQEILMNFYENRQNPKN"
     gene            complement(7260..8117)
                     /locus_tag="PK683_00100"
     CDS             complement(7260..8117)
                     /locus_tag="PK683_00100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNH06870.1"
                     /translation="MSEKGKSNVKVMPIVRKPHFNWEGMPLHYVENNYFGTHFLNSMH
                     VVFPEGEKFFIRSVKAFVDKIEDPNLKERVKAFIGQEAQHMALHKTFWQKLQDDGPAL
                     EIFEKIYSATAFDFLEKISRALSRDKYSLSVTAALEHYTAILAEVVFEKDSEILDGIP
                     EEMVKMLKWHAAEEIEHKAVAFDVLKTVDDSYLLRVSGMIFATYGLVFYILMGQAIFM
                     ALDKQIPFWDMPGQFLRFAEKAAPIFTAAAREVADYFRPNFHPDDKDNISIAKEYFAK
                     VEEELKAAV"
BASE COUNT         2203 a         1891 c         1591 g         2769 t
ORIGIN      
        1 gggtcggatg ataccataca aatttcttta ttctgtccag attgactccc ggaggaagtg
       61 catccagaac ggactgcttc attctgtctt cgcgcggacc aatatgggta agattgatca
      121 ggacggccct taccggattg cggtttccgt gaatggagat attttcttct gcaagagaac
      181 ggattctttt tgcctgatcc ctcgttacag catctcttcc cagaaaattc agacggtaca
      241 gatagtcctc aagccttttt ccttcaattt cccccattaa tatcaattga tataatgtaa
      301 atataaaata atcactttcc gtattatctc cgagaaggat ttcctttgct ttggacggct
      361 gatagattct gtcctcaaga aggattgtca gcttgtagga catctgatca aaaagactga
      421 gataggaaga gtgaaagaat ttttttactc tttccactgc aggctggatg ccttccttca
      481 gaatctgatc aggccttgtg atcgaagcca ctaccttgtc cacgacaccc ttcactgttc
      541 ctgccagata tttcaggtgt aaagattctg tctgaactcc atgatgcttg aatgtggaaa
      601 gcagacttct tctgaaaaaa tgagggcttg ctgaaataaa acaaagtgga gtgtcttcgg
      661 tgtcccttct cagtgtccag taaagttcag gcattcccgg aagaaaaaat ttctcattgg
      721 gtgtttcaaa cagagtggag agcattcctt ttttggatcc cagttctgtg gccaagtagg
      781 tctgatcaat atccgaggtt gtgataaaat cgttgtagct ttcgggaaga accctcatct
      841 gaccgattcc tacgatagtg gactttgtga tatcactctt gtctctgaga ttcatatagg
      901 cgatatcctg agtgtactgc ctgtagctgt cgattccccg aaaaatgaca tggaatgtgt
      961 atttccccgg tcttagcttt tttctaattt cataggaaaa gaaaccatct tcgtttccgt
     1021 tgaaattttc cgattcaaag accttcttgt ctttctcgtt aaaaagctca atggaaagga
     1081 tcggctttct gacaggagcc agccccaaat ccttgaaagg agttacatca ttttcttcac
     1141 ccttaaaaaa gccggttaaa aaatcccaaa gatccgctgc ttccgcaatg tttggaacac
     1201 cgatatccac cagctgaccc cgaaccaaag accgctgttc tctgcccaga gaacccccgc
     1261 atactgctat cctttttttg tcccgaagtc tggcttctct tgttttaggc tgtatgtgtt
     1321 cttcgtattc catttgctgc tccaggccgg gttttggtga aaatctatga tatttctaga
     1381 ctgcagattc atatttcaca gatcaagtat ttttatccga aatggcctta taagccgtct
     1441 ttttcgatct tgtactgtta atagtgaagg gaacgtttta gggagtgaaa atcctggact
     1501 gatcgcggaa aaaagaaaaa agatgagttg acatcaaagc aaaatcattg cctctctgac
     1561 acttctttag actgaattct ttctgcggaa cggctcggaa aaaatcttgg agccgccgtg
     1621 gatcgtggtc gtgcagaaag aaatcttaga taacgctgaa catcaatgat gctttttctt
     1681 atgctttttt gcttttgcag gtccgcaatt ccattttccg tttttggatt tcatattgca
     1741 ctgaggcagt tttgcggatt tggatgcctt gctcattttt cccttttcca ttttcattcc
     1801 gcagcatttg cagacctgtg cttttgcttt cggtttatgg cctttgtgac tttttgcgga
     1861 caatccgctg gataggaaga atgctattac tcctgccaag acagttctaa aaacggtttt
     1921 cttcattctg gttccttctt ttaaatatgg tttcctacta taaaaaaaca ctctttcctg
     1981 atttcaacat cttaatgcga ctttgtcaag ttgcataaat cacatcagcg aaatggaaaa
     2041 acgcccgcag gaatggaggg tgttttatcg aaatatgttt tctgaataca cgcttttcca
     2101 ctcctcgtcc ggaagacttt gtcaggacag agaaaaagat catttcacca aagaagaaac
     2161 ggtatatttt gggattcaga tttgttccag taaaagaatc atttgatgca agtttttatg
     2221 ggatgcttcc tggattttca gggatagagg catatcacga agcctctgca gatgaaactg
     2281 ctgaaactca aattccaaaa tctgagccga aaacaatgtc tgaggcgatt tcggaaggta
     2341 tgagcagagc ttctcaagat tcaattttgg tcaattcaat cgtccgcgaa gtaacatgga
     2401 atattgagag aaattcagaa aagtatgggg ttctagtttc ctcaggttca tcacgtctat
     2461 atgatcctta ctatcgaaag tctgatcctg tttttgacta ttctgtaagt ttcaaagtca
     2521 agcaggtgat acattcatat aaaatgcact cttactacat ctttcctttc actgggtttg
     2581 tctggatatt ttcttccaat ccaatgcagg aaatatatca cagagctgtt ctggaaatga
     2641 cagtaaaaga cagagacggg aagacagtga aaataaagga gctgaatgtc cccgcttatg
     2701 aacccctata tttttggaaa ttcaactggc tgaaaaccgt tgatgacaga cgatgagatg
     2761 cggaattttt tccacatctt ttaccaaaat ggagtaatat tccgttttgg tcaagtgatt
     2821 tttcaccaga cagcgtaatg ttcttcgacg agtccgaatc cttttatttt taatgttttt
     2881 ccattataaa gtatgtttcc ttcaaatctg ccgtaagcct gtacaaatct gctctgaaag
     2941 agaaccaggt tgatgtcttc agatcttttt ccttcgggga tgaaacgaaa ttcggagagt
     3001 ccatccattg taatgatctc gcattcgttc tgtgagatcg gctttttgta actgaacttg
     3061 gctatgccca gaggaagaat ttctccgttg atccaggcat tgttttcgag tccctcattg
     3121 aatcctgcaa caagattcaa tgcaaaagat tcccctgttt ccgcctttcc tgaaaaactt
     3181 gcccagttcc agaatgtctc tcttccagga taaccttttg aaaaatcaac tgttccgcag
     3241 tctgcagtcc agctgtgttc ttcctttccg atttttattc tgacctcagc agggaggttc
     3301 atatttttat atgttgtatg aaatggtctg tcttttgcag gagcgatcgt gctcattccg
     3361 ttttcatttt cttggagaac atattttaat gatattttat ctcctgaata cagaatggaa
     3421 taaccgccct ttacaggcat aaactcccag acctgatttc ctttttccag tttccatgcg
     3481 tcctgaagtg agggctggaa ttcatccgaa aaaccgagta tcttgtccat tttttcttct
     3541 aggaaaagtc ccgtttttct ctggtaaata taggcaaatg cagtcgcaat cagtcctgca
     3601 tccacaattg caaatccgac agtgaagtcc tcagtgatca gtcctgaaaa aatccacgcc
     3661 tttctctgaa gccttctttc ggaaagatat cctttgttcc cgtcccattt tgaagaatca
     3721 aaattcagaa gtccctgata tcttccaaaa tgttttgtca gatcccctga tacagaattc
     3781 actttttcta aagcactcat agcatctcct gtcagtaaag atctgacata tccgtcaaat
     3841 tatcataaga acttgacttg tcaacaaagg agatcgaggt aattgaaaat cctgtgactc
     3901 ctgtatcttt tttttacagg gtttgattcc ttatttcgat acttttttta aatcgattct
     3961 ccagcttctt tgcaaatgcc ttgaatgata aaatctgata tatggtagtt cattttttca
     4021 gttcctgaag caatggggaa acttgattga tgattccaga ttcttttgct ttcagtaaaa
     4081 ttcctgctgt accaattaca tttatgccat acacgttctt agcaatattt cttccttttc
     4141 tttcatcaat gatcagccag tcagcattct tctttaaagc taatgtgatt gcctccgctt
     4201 ctctgcgatc cagctctttt tccaaaaggg ggtcaacgaa tgaaatctct tctgttttaa
     4261 aaacggattc ttttaaattg atctcatctt tatattttaa attgttaagt tccagaaata
     4321 cataactggg aattataatt ttcccaaata ctgcttctag aatggaaaat ctattcaatt
     4381 tgccaaatgc aattactgga cttgtatttg aaacgacaat cataacttac gggaattctt
     4441 gatttcttct tccaattcct ctgtgttcat ttgaacaacc gggattcctc tgcggcttaa
     4501 atcagaaata aagtcatatc gattcatacc gcacatttct gctgcttgtc ctgtagtcaa
     4561 aaaaccttct tcaaacagtt tgacagccag cagtttctga gcttctgctc tgattttttc
     4621 ctttgaccca tcccatttca aaagcagtga atctggaact tctatcatta tacttaacat
     4681 cctgaattct cctaaccatt acatcctgtc tgtccatagt gaaaatcccg tgaatcctgt
     4741 atcttttttt ttacagtatt cacaggatca ggaggtgcat tatttcagta tatattcctt
     4801 gggctgaaag ttttctgttc tgttcctgaa ttcaaatgtg aatcccggcc agagtgttgt
     4861 attctttccg tccttcgttc tgtaccagct gacacagcct ccgttgttcc atacagaatt
     4921 tcccagccgg gactgaactt ctttgttgaa ttcttcctga atctcaggtt ttacttcgag
     4981 catattcagt ttttcttcct gaaggtactc taccgctttc attgcataat tgacctgcga
     5041 ttcaatcata tacaccattg agctgtgtcc aagcccggta tttggtccca caatcataaa
     5101 catattagga aatcctttta cagttgttcc gagatatgct tctggtccgt tcttccattc
     5161 atcattaaga gatatcccat tttttcccaa aacaggaaac gggagaatgt cttcagctgc
     5221 cttgaatcct gtcgcagcga caatgatgtc cgcgtccact ctggacccgt ctttcaggat
     5281 gattccattc tcatccattt ctgcaattcc ttctgtgacc agatctacgt ttgatctctg
     5341 aagggcaggg taatagtcgt tggaaagaag aattctcttg catccaattg tatagtccgg
     5401 agtgagtttt gctgcgagca cgggatcttt cacacagttg cgtatatgct tttcaatgag
     5461 tctctgaaat gctttcatga ctgcgggcgt atatacaaat gccattgctc ttgcttccag
     5521 tctccagtag atggcttctc tcgatgccca ctgaagagga ggaaacatct ggaatgcatt
     5581 tttttctata ttggaatacg gtctgtcttc cttggggaga atccaggatg cagtcctctg
     5641 aaaaaccgta agtttcttta ctttcgatgc aattgcggga acaatctgta ttgcagatgc
     5701 tgcagaaccg atcactgcaa cttttttgcc ttttacatcg gcactgtgat cccattttgc
     5761 agaatgaaag aatttcccct tgaacttttc tcttcctttg atatcaggaa gcacaatctg
     5821 gctcagtcct cccagtccga agaatgcata ttttgcctga atgatatcac ccttgtctgt
     5881 tctgatttcc cagcgttttt ctgaatcatt ccattttgca tccactgctt ctgtattgaa
     5941 gcggatatgg ggattgattc catactttga agaacagtgt tccatgtact ccaggatttc
     6001 cttctgcatt ccgtatgtgc gcgaccagtc cgacttgtgc tcaaaggaaa atgaataaag
     6061 atgggactga acatcacact gtgcccccgg gtaagtgttg actctccaga ctccgcccac
     6121 gccggaatct ttttccaata caataaaatt cattcctttt tctttaagtt ttattgcggc
     6181 gcagagtcct gcaaatcctg ttccgatcac aactgcatct gctgtgtttt tcataatgtc
     6241 tcctcttgtt acttctttca ggtgttttcc tttttctgtc attgtctgcc tctgtcagtt
     6301 ttttggattt tggcggttct cgtaaaaatt catcagaatt tcctgaacct ctttaggctt
     6361 ttccatatgg ggaagatgac ctgtttcatc cactacctgc agtgtcgcat gaggtatctg
     6421 ctccttaaac tgatgagcaa ttttctcagg gaagatgccg tcatggctgc cccagagaat
     6481 cagcacagga cattcaatcg aagaaatttt ttctctctga attctctgat gctcctcttc
     6541 atttttccct acatattttc ccagtatatc gttcatcagc ttgtcgtacc aggcaccgtt
     6601 gtgtctgtac ttctgaaaca tataatgttt tacagtatga ggaaccatat tctttttgta
     6661 gaaaagtttg cctacgaact cttcgaaatg gtattcttcc tcaatgatga agatattttt
     6721 ccccgatctg taatcatcgt aaaaatggtt tccttcctcc tccggcacaa gtcctgccgc
     6781 gcaggaaagc acaagagatt ccaccgaatc cggatgtctg tgcgcatatt caatcagaat
     6841 tgctcctccc atggaattgc ccagagaatg aaatttataa acctttttat aatttagaaa
     6901 ttccagaagc cattccgaat acagctccag agaataggta cagttcggct tgctgctttt
     6961 tccgaaaccg ggaagatccg gaatcagaat atgaaagtcc ctggcaagat ggcctgccag
     7021 gctgagaaaa ccttccttgg aatcggaaaa gccgtgcaca atcaccaaag tcggtttgcg
     7081 gatgtcccca agttcaatat aggagatttc aacatccctc agctgtactg ttttttcctt
     7141 tgctccggtc gaaatgtaaa gtaccttatg attcaggttc caaaggaatt caccgatccc
     7201 tttcctgatg tcatccggaa caagcccgta catttcgtgg aatgttttct ctatcgcgat
     7261 cataccgcag ccttcagttc ttcttccact ttcgcaaagt attcttttgc aatgctgata
     7321 ttgtccttgt catcaggatg aaagttcgga cggaaataat ctgccacttc ccttgcagct
     7381 gcagtgaata taggagcagc tttctcggca aatctgagaa actgaccggg catatcccag
     7441 aacggaatct gcttgtccag tgccataaaa attgcctgtc ccatcaaaat ataaaaaacc
     7501 agaccatatg tagcaaatat cattcctgat acacgcagaa gataactgtc gtccaccgtt
     7561 ttcagaacat caaatgcaac agccttgtgc tcaatttctt cagcagcgtg ccatttcagc
     7621 atcttcacca tttcttccgg aatcccgtcc agaatttcag agtccttttc aaaaaccact
     7681 tctgcaagaa tggctgtgta gtgttcgaga gcggcagtca ccgaaagaga atacttgtct
     7741 ctgctcagag cacgggaaat tttttccaga aaatcaaatg ctgtcgcaga atagatcttt
     7801 tcaaaaatct ccagagccgg tccgtcatcc tgaagtttct gccagaaagt cttgtgcagt
     7861 gccatatgct gtgcttcctg tccaataaac gctttcactc tttccttcag attcggatct
     7921 tcaattttgt ccacaaatgc cttcacgctt ctgatgaaga atttttctcc ttccggaaat
     7981 accacatgca tactgttcag gaaatgtgtt ccgaaatagt tgttttctac atagtggagc
     8041 ggcattcctt cccaattaaa atgcggtttt ctgacaatcg gcattacctt gacgttggat
     8101 tttccttttt ctgacatggt ttcctccaga ggttccctct aatttgctga acatcgcgtc
     8161 tttcttaagt tgacgttctg ttcaactttt tgtatagttg acgctttgtt caacttttgt
     8221 caatcaaaaa agtacatttt tcagtaaaaa aatcctcctc ctacgaaaaa aaggagttcc
     8281 cacagtcgaa ttatgtcgga ttaagggggc ttttaaggca gaacgttcat ttccaatgga
     8341 tgttttattg attctagtgc ctgttttaca taatttccgt tttgtataag aggatcatca
     8401 gcacggacgg tcagttttta ttgacatact tacagagtat aaaaaaaatc ctcc
//
LOCUS       DAOQGJ010000003         8231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_4133_length_8231_cov_5.138821, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000003 DAOQGJ010000000
VERSION     DAOQGJ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_8231_cov_5.138821"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PK683_00105"
     CDS             <1..450
                     /locus_tag="PK683_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06871.1"
                     /translation="PDIEKDTVLILNGHPYPGTLDAADTEHLRNFLGKKCVYFYNDSY
                     NEITEHSDHYKISTPFIYPIDSCADRTVESLEFFYEPMKRYFWTIYWDKKTVEVPKDK
                     VIYLRWDNEKQSMSLVHSKSNKSRILKAAGNKYVDDLLNIDSVLHNK"
     gene            909..1868
                     /locus_tag="PK683_00110"
     CDS             909..1868
                     /locus_tag="PK683_00110"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH06872.1"
                     /translation="MIENRKIFITGGAGFIGSHIIDRLIENNKIVVYDNLHRNAIKFT
                     GSLNHPNLTFIQGDVLDLEKVKSSMKGADFVIHAAAIAGIYSVGVKASNTMKVNLLGT
                     FNVLEACVENNIKRLIDFSTSETYGPFIYKGKESDKTSLGPVGEKRWIYAVSKLAGEH
                     MAHAYADDFGLEVVTVRPFNVYGPRQIGEGAIQQMANRALRNEDITVFNDGTQIRSWC
                     FVTDFADCIVKCLDVADAKNNVFNIGNPQATLTVFGLAEKIIQFTGSKSKINFKPHPG
                     PEVEMRIPDIGKAREILNYEPIVGMDEGLKLAIDWYKQHQITV"
     gene            1865..2797
                     /locus_tag="PK683_00115"
     CDS             1865..2797
                     /locus_tag="PK683_00115"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH06873.1"
                     /translation="MKIIVTGASGFLGKNYLLSTNPNDEVFAVYNNSADFLSFLHDKE
                     LSWVRPLKVNLEEPESISKSMTKGEKFDKCIYFAANGDPAVSVTKVGFDLRSNAVALV
                     NLLEHIQAGRVVFFSSGAVYDGVGGGVSYESHKSVSPHLPYAVSKLASELYIHFFQTK
                     GYIDSYANVRFFGAFGPYEPERKIYTKLINSFAFQKSIQFSIRGNGKNFIDAMYIDDA
                     VRAVKILENSNESLGLDLYSGQKKSISELVTVVAHFFGIEPEIQYVGEVPEYIEFYSV
                     DDTMKKKFSFHCETDLLEGIKKQYEFLNEQRRTK"
     gene            2794..4230
                     /locus_tag="PK683_00120"
     CDS             2794..4230
                     /locus_tag="PK683_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNH06874.1"
                     /translation="MSQETVIILGAGPAGLAAAYKILKNSKKKVIVIDKAKVPGGSGA
                     SFQWKNHVLDYGPHAFHTRGDEPELLIRDLFKDSPDHLTNGIKKVSIYLKRKIFKYPL
                     QVKQALLKFNPLLSLRIMIEFALTSIFHGIVSIPIESFENWGKKRFGSTLYKISFGDY
                     TEKVWKTHPNKISTKFASEKIQGFSFINLIQKLLRIGGQVTEPYYQTWIYHRKGSGQL
                     YIKMAEEIKKLGGEILLETNIKFIEMKDHRFASIAFEKDNAAKSLPIDFLINSIQIPA
                     FVNLIKGNLPYSVQFSSKKLRYISLILVYLEFETDKISDNHWFYLLENNFASNRVTEQ
                     KNLSPETIESGKTVLSFEVTCHLGDETWQKSDKELIDLVIKDCEGINFLQDKIPKISD
                     TLVKRVPNVYEIYYKQFDSHANLLFSYLNEFENFVTVGRRGLFLQGDMHQAVEMGLNI
                     ASIASKDSIDKKEIEGFYKKYLKYIDIF"
     gene            complement(4982..5152)
                     /locus_tag="PK683_00125"
     CDS             complement(4982..5152)
                     /locus_tag="PK683_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06875.1"
                     /translation="MTIGKTAKGIMPKYTIKSDVAKMDERICSFKWLDLKLRKRIVAT
                     NGKAVIAAFCPT"
     gene            5487..7043
                     /locus_tag="PK683_00130"
     CDS             5487..7043
                     /locus_tag="PK683_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06876.1"
                     /translation="MAYWFFRNWKSERAVVLSALLVSAFLSLGRYFIADNNYALFKIL
                     AWFQFLFIPFLYFFISESKIRNLLNYTSVFLKQFQLKLLRDDAGFAVLWRVPKISIFS
                     LNGKSWFFPVCTFLAIVLVWLNLINSNFLLKSSMGLDNRDRMVAAWGISGTKDMEDLK
                     KSISLTVKKDETVAINMENFIWNFWVSYLLHGHSNYIITNKKLPIFETKLPDPITQKY
                     IDPDGIPRVEPKFEVLDADYILTWNTTAVHTDIVEQSPLIKPIWQNKTFRLYKRTDLE
                     TYIFVGKGYRKVEYDSKKVKSYYWPNSYRCSDNGGEVYVYANDLNKSYYVNAGIYPGA
                     EKNFIEVYYDEKLVSKHLIYKLGKLSIGPLKFKHQVNKLTFKSLDNTLEVCFSGFKVW
                     DGKNSPIGVLSNLKEVIEKSFHLENFELEGWVNNSTKMLYRFNQKGERVRLKIFVPGD
                     RGLKFPFYISGKVNNQNFSYIADAPGFDTIYIKLNESTNDFTFQYNNLEEFDEVGQKR
                     SFIVHGIEFR"
     gene            7145..7558
                     /locus_tag="PK683_00135"
     CDS             7145..7558
                     /locus_tag="PK683_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06877.1"
                     /translation="MKISELSSYGNINYNYNFDHMIMEKWSNDSENSAIHYFYTYKTD
                     LNKLENNGCPVSIGLNFFDPGSEADFKDRFASFIKNAVSSSEVFLDFYQKNADFFIKD
                     IKSKELMSISDNIIFKMEILVYDSKKIYSVEFKSK"
     gene            7575..>8231
                     /locus_tag="PK683_00140"
     CDS             7575..>8231
                     /locus_tag="PK683_00140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH06878.1"
                     /translation="MNLGILSPVYNDWVSANVLYKEIHKILDLTKIRPFYFFIDDASM
                     NYEGMEDLRKHKNKNIEIIYLNTNLGHQRALAAGLSYLSKEKNLDAVVILDSDGEDNP
                     EYINDFISEFLKDRNRIVVAERTKRSESLKFRAFYQVYKFLFKLLTGKSISFGNFSLL
                     SKFILDRIVHQSDLWNHIPSALIRSNFPIVKVKTIRSKRYFGESKMNFFSLVLLGISA
                     F"
BASE COUNT         2644 a         1299 c         1605 g         2683 t
ORIGIN      
        1 ccggacattg agaaggacac tgttttgatt ttgaatggtc atccgtatcc aggaactttg
       61 gatgcggcag atacagaaca cttacgaaat tttttaggaa aaaaatgtgt ctacttttat
      121 aatgacagct ataatgaaat tacagagcat tcagatcatt ataagatatc tactccattt
      181 atatatccta tagatagctg tgcagatcgg acagttgaaa gtctggagtt cttttacgaa
      241 ccgatgaaga gatatttttg gacaatttac tgggataaaa aaactgttga agtgccgaag
      301 gataaggtga tttatcttag atgggacaat gaaaagcaga gtatgagcct ggtacattct
      361 aaatccaata aaagcagaat actgaaagcg gctgggaata agtatgtcga tgatctgctg
      421 aatattgatt cagtcctgca taataaatga atctctgcca gcagaacctg aaagattttg
      481 ctctgaagtg aatcttgaaa atgtttcagg aaaacgaatc ctgtctgaaa tcaaagataa
      541 acaggacaag aaaaatgctg ttcaagaact gatgtttgca caaagacaaa tgcaggattc
      601 ctaaagaaat tatactatgc gaaaataatc ttggataaat aatggtacat actaaatgtc
      661 ggaattcctt tcggcatttt ggcagtgttt tagccgactt ttaggctagc taagacggtt
      721 ttcctttagc agctttcaag ttcgcaaaac aatttgtcgg tttcaagaat cagtaaccgt
      781 gaagcctgtg gagtagtctg tctagtctcg ggaaggaatg tgacgtttgc ttcagtaagt
      841 gaaacaggct tttgtcgagt ttgtttcatt aagttgctga ttatgacaat acacaaagga
      901 gttttctctt gattgaaaat agaaagattt ttattacagg cggtgctggt tttatcggtt
      961 cgcatattat cgatagactt atagaaaaca acaaaattgt tgtttatgat aacttgcaca
     1021 gaaatgcaat aaagtttacc ggctcactga atcatcctaa tctgactttt attcaagggg
     1081 atgtgttgga tttggaaaaa gtcaagagtt cgatgaaagg tgcagatttt gtaattcatg
     1141 cagctgcaat agccggaatt tattctgtag gcgtcaaggc ctccaataca atgaaagtaa
     1201 accttctcgg gacatttaat gttttagaag cgtgcgtaga aaataacatt aaaagactga
     1261 ttgatttttc tacaagtgaa acctatggcc catttatcta taagggcaaa gaatcggata
     1321 aaacctcctt gggtccagtc ggagagaaga gatggattta tgcagtcagt aagctggcag
     1381 gagagcatat ggctcatgct tatgctgatg actttggtct tgaggttgta accgtcagac
     1441 cgtttaacgt ctatggacca agacagattg gtgaaggagc cattcagcaa atggcaaata
     1501 gagctctcag gaatgaggac attacagtat ttaatgatgg aactcagatt cgttcctggt
     1561 gttttgttac tgattttgcg gactgtattg taaaatgtct ggatgtagct gatgcaaaaa
     1621 acaatgtttt caatatcggt aatccacagg caacgttgac ggtctttgga ctggcagaaa
     1681 aaattattca atttacaggt tcgaaatcaa aaattaattt caaaccgcat cctggtcctg
     1741 aagtggaaat gagaattcct gatatcggca aggcaagaga aattttgaac tacgagccta
     1801 tagtcggaat ggatgaagga ctcaaacttg caattgattg gtacaagcag catcaaataa
     1861 ctgtatgaaa atcatcgtaa ccggtgcatc aggattttta ggtaaaaatt atttattatc
     1921 tacaaatcct aatgatgagg ttttcgcagt ttacaataat tctgcagatt ttctaagctt
     1981 cctccacgat aaagaattat cctgggtaag acctctgaaa gtaaatcttg aagaacccga
     2041 gagtatttcg aaaagtatga ctaaaggcga aaaatttgat aagtgcattt actttgcggc
     2101 aaatggtgat cctgcagtct ctgttactaa agtgggcttt gacttaagat caaatgctgt
     2161 tgcgcttgtt aatctattag agcatattca ggcaggcagg gtagtatttt tctcttctgg
     2221 agctgtgtat gacggtgtag gaggaggagt ttcgtatgaa agtcacaagt cagtaagtcc
     2281 tcatcttccc tatgctgtgt caaaactggc gtcagaactg tatattcatt ttttccaaac
     2341 aaaaggttat atagattcct atgcaaatgt aagatttttc ggagcttttg gtccttatga
     2401 accggaaaga aaaatttata cgaaactgat aaacagtttt gcctttcaaa aatccataca
     2461 gttttcaatt cgcggcaacg gcaaaaattt tattgacgca atgtacattg atgatgccgt
     2521 ccgtgcagta aaaatacttg aaaattctaa tgaatcattg gggctggatt tgtattcagg
     2581 acaaaagaaa agcatatctg aactggttac tgttgtagct catttttttg gcattgaacc
     2641 ggagatacaa tatgtcggag aagtacctga atatattgaa ttttattcag tagacgatac
     2701 aatgaagaag aaattcagct tccattgtga aactgatctt ttggaaggga tcaaaaagca
     2761 gtacgagttt ttgaatgagc aaagaaggac taaatgagtc aagaaacagt cataattctg
     2821 ggtgctggtc ctgctggtct tgcagcagcc tataagatac taaaaaattc aaaaaagaaa
     2881 gtcatagtca ttgacaaggc aaaagttccc ggtggatcag gagcaagctt ccaatggaaa
     2941 aatcatgtac tggactatgg tcctcatgcc tttcatacaa gaggtgatga acctgagctt
     3001 ttgatcagag atttgtttaa agacagtcct gatcatctta ctaacggcat taaaaaagta
     3061 agtatttact taaagagaaa gattttcaaa taccctcttc aagtcaagca ggctctttta
     3121 aagtttaatc cactgctaag cctaaggatt atgatagaat ttgctttgac ctctatcttt
     3181 catgggattg tctctattcc tatagaaagc tttgaaaact gggggaaaaa aagatttggt
     3241 tctacactgt acaaaatcag ttttggggat tatactgaaa aagtctggaa aactcatcca
     3301 aacaaaatct caaccaagtt tgcctctgaa aaaatccaag gcttcagctt tatcaatctg
     3361 atccaaaagc tccttagaat cggcggacaa gtgactgaac cttattatca gacttggata
     3421 tatcacagaa agggttcagg tcagctgtac ataaagatgg ctgaagaaat aaagaaactg
     3481 ggaggtgaga ttcttttaga aaccaatatc aagtttatag agatgaaaga tcacagattt
     3541 gcaagtattg ctttcgaaaa ggataacgca gcaaagagtc tgccgattga ctttctgatc
     3601 aattctattc aaattcctgc attcgtaaat ttgattaaag ggaatctccc gtattctgtt
     3661 cagttcagca gtaaaaaact tcgatatatc tctctcattc tagtttattt agaatttgaa
     3721 acagataaaa tctctgataa tcattggttt taccttctgg agaataattt tgcttccaac
     3781 agggttactg aacagaaaaa tctgagtcct gaaacgattg aatctggcaa gactgtttta
     3841 tcttttgaag ttacctgtca tttaggcgat gaaacttggc agaaatcaga taaggaactt
     3901 attgacttgg ttatcaagga ttgcgaaggg ataaactttt tacaggataa aatcccaaag
     3961 atcagtgata ctttggtaaa aagagttcca aacgtttatg agatatatta taagcagttt
     4021 gattcgcacg caaatctgct gttttcctat ctgaatgaat ttgaaaattt tgtgaccgtc
     4081 ggaagacgag gccttttcct tcagggagat atgcaccagg cagtggaaat gggtttgaat
     4141 attgcgtcca ttgcttcaaa agacagtatt gataaaaaag aaatagaagg attctataag
     4201 aagtatctga agtacataga tattttttag gtttttcaaa tttgatggat tattcttata
     4261 atattatcca gattttatta agttactttg tgacttatat gaatgggtac tggcttgtaa
     4321 caatgacttt acccagaaga tatgttcaat acagatttat aataatgcct gcagcgggct
     4381 ttttatccgg atgtctgttt gttttctgtt actcctccat atttcagatt tcagtgctgg
     4441 atgcagccaa tctgcatata atgatcacat ttattctcaa tgcggcagta ggtatatatt
     4501 ttcataaatt atatacctgg aaagccagtt tccttcaaat tcgctcttta ttaaaatttc
     4561 tgccgattct gttcctggcc ttttatcctg ttttaatgtt cggttatgat aaatattttg
     4621 gaattgtaaa tccagatatg ttcgtaattc tcggcgacaa tacctttctg ctgaatcatt
     4681 cagtgacttc cttgaagaca ataccgtcgg atatcaatag gtccaatttc ggtgccttta
     4741 tgggacaaat gcccttgtct gcaaggtttg gcgcaaactt attttcattc caattccata
     4801 ttctattcag ggccagtctt gaagcatcaa tcagtctctc aattgtaaat tttttgagct
     4861 gtatgatttt agtgtcctat tttttcacta aggtattttt cctttcaaaa agaagctttg
     4921 cttacattat ttcagcactt actcagatat catcctgcac tgtcttgagt tttgttactt
     4981 tttacgtagg acaaaatgct gctataacgg cttttccatt tgttgcgacg attctttttc
     5041 tgagcttcag gtccaaccac ttaaaactgc agatcctttc atccattttg gctacatcac
     5101 tttttatcgt atacttcggc attattcctt ttgccgtttt tccaattgtc atcttcgctg
     5161 tcttttatct gtttcagaag aaaacaagtc taaaaaatct ggggctgttt gtcggaattc
     5221 atttattgcc gctgatcatt attttatcca taaattatga atatttttct gtttcattgt
     5281 tacaatggtt ctatttaata aagacaaatt taaagattca gttctttgaa ggtctggatg
     5341 attatcgata catatcaatt ttattcggag cactgacgct gcctgtaaat ttttctgtag
     5401 tatttacaaa gccggctctg aataaatata tctacagtaa tctgtatatt ataaacatag
     5461 tatacctcat catgtttttt ggcagtatgg catactggtt tttcaggaac tggaaatcag
     5521 agagagctgt cgttctttca gcgttgctcg tttcagcatt tttatcatta gggagatatt
     5581 ttattgcaga caacaattat gctttgttca aaatactggc ttggtttcag tttttattta
     5641 ttccattttt atattttttc ataagcgaga gtaagatcag gaatttactg aattatacgt
     5701 cagtttttct taagcagttt cagcttaaac ttttaaggga tgatgcaggg tttgctgttt
     5761 tgtggagagt tcctaaaatt tccatatttt ctttgaatgg gaaaagctgg ttttttcctg
     5821 tatgtacttt tctggcaata gttttagtct ggttaaattt aataaattca aactttctgc
     5881 tgaaatcatc aatgggtctg gataacagag acagaatggt tgctgcctgg ggaatttccg
     5941 gaacaaagga tatggaagat ctgaagaaat ctatttcctt gacagttaag aaagatgaaa
     6001 cggtagccat caatatggaa aatttcattt ggaatttttg ggtgagttat ctcctgcatg
     6061 gacattctaa ctatataatt acaaataaaa aacttcctat atttgaaaca aaactgcctg
     6121 atccaattac tcaaaaatat attgatccgg atggaatacc gagagtggaa ccaaaattcg
     6181 aggtacttga tgcagattat atccttactt ggaatactac agcagttcat acagatatag
     6241 tagagcagag tcctctcatt aaaccgattt ggcagaataa aacttttcga ttgtacaaaa
     6301 gaacagattt ggaaacatat atttttgtag ggaaagggta ccgtaaggtt gagtatgatt
     6361 caaaaaaggt caaatcttat tattggccga attcgtaccg atgctccgat aatggcggag
     6421 aagtttacgt ttatgcaaat gatttaaata agagttatta tgtaaacgca gggatttacc
     6481 caggggcaga aaagaatttt attgaagtat attatgatga gaaattagtt tctaaacatt
     6541 taatatacaa attaggaaaa ctaagtatag gtcctttaaa attcaaacat caagtaaata
     6601 aattgacatt caagtctctt gataatactc tcgaagtatg cttctccggt tttaaagttt
     6661 gggatgggaa gaattctcct atcggggttt tgagcaatct taaggaagtt atagaaaaat
     6721 cttttcattt ggaaaatttt gagcttgagg gctgggtcaa taattctaca aaaatgcttt
     6781 atcgatttaa ccaaaaagga gagcgagtaa ggctgaaaat ttttgtaccc ggcgatagag
     6841 ggttaaagtt tccattttat atttcaggaa aagtaaacaa ccagaatttc agctatattg
     6901 cagatgctcc cggttttgat actatatata ttaaattgaa tgagagtaca aacgatttta
     6961 cgttccaata taataacttg gaagagtttg acgaagtcgg tcaaaaaaga agtttcattg
     7021 tgcatgggat agaatttaga tgagaaagta tttatatatg acattcatca ttctcgtcaa
     7081 ttgcaggctg tcttacttgg gcagtaaatg tcctaagtcc ccattcataa cacctgataa
     7141 agttttgaaa atttctgaat taagctcgta cgggaacata aattataatt ataattttga
     7201 tcatatgata atggaaaaat ggagcaatga tagcgaaaat tctgccatac attattttta
     7261 tacttataaa actgatctga ataagctgga aaataacgga tgtccagttt ccattggtct
     7321 gaattttttt gatccagggt ctgaagctga ttttaaagac cgattcgcat cctttattaa
     7381 aaatgccgtc tccagttccg aagtcttttt agatttttat cagaaaaatg cagatttttt
     7441 cattaaagat atcaaatcaa aggagttaat gagtatttca gataatataa tatttaaaat
     7501 ggagatcctt gtatatgaca gtaaaaaaat ttactccgtt gagtttaaat ccaaatgagg
     7561 tttaaatgag gactgtgaat ttaggaatac tttctcccgt atataacgac tgggtttcgg
     7621 caaatgttct ctacaaagag attcataaga ttttggattt gacgaagatc agaccctttt
     7681 atttttttat agacgatgca tctatgaatt atgaaggaat ggaagatctc aggaaacata
     7741 aaaacaagaa tatagaaatc atttatctta atacgaattt aggccatcag cgggctttgg
     7801 cggctggttt atcttacttg agtaaggaaa aaaatttaga tgctgtagtc atattagatt
     7861 cagacggaga ggacaatcct gagtatatta atgattttat aagtgaattt ctcaaagaca
     7921 gaaacaggat cgtcgtagca gaaagaacaa aaagaagcga gagtcttaaa tttagagctt
     7981 tctatcaggt atacaaattt ctttttaaac ttctgacagg caagagtatt tcatttggaa
     8041 atttcagttt attgtccaag tttattctcg accgtatcgt gcatcaatcg gatctctgga
     8101 atcatatacc ctcagcatta atcagatcaa actttccgat agttaaagtt aaaaccattc
     8161 ggagcaaaag atattttgga gaatcaaaaa tgaatttttt ttccttggtt cttctgggga
     8221 tttccgcatt t
//
LOCUS       DAOQGJ010000004         7773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_4501_length_7773_cov_3.635786, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000004 DAOQGJ010000000
VERSION     DAOQGJ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_7773_cov_3.635786"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2667
                     /gene="secA"
                     /locus_tag="PK683_00145"
     CDS             <1..2667
                     /gene="secA"
                     /locus_tag="PK683_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNH06879.1"
                     /translation="KRLLPIVAKINALEDKISAMSDSELRAQTAKFKELLEKKQTLDD
                     ILPEAFATVREASKRTMGLRHYDVQLMGGIALHWGNIAEMKTGEGKTLTSTLAVYLNS
                     LSGLGVHLVTVNDYLARRDANWMRPIYEFLGITVGIIQHDMDYEERKAAYGCDITYGT
                     NNEYGFDYLRDNMVERKEHKVQKHHNFAIVDEVDSILIDEARTPLIISGPAEESTDKY
                     IRVNAIIPKLVEKEDYEVDEKARNVLLTENGVLHSERLLGVDNLYAPENVELVHHVHQ
                     ALKAHKIFQKDVDYVVQNNEVIIVDEFTGRLMAGRRYSDGLHQALEAKERVTIARESQ
                     TLASITFQNFFRIYTKLSGMTGTADTEAEEFAKIYKLDVIVIPPNLKMKRIDNPDRVY
                     RTGKEKFNAIVNEIKDCYTRKQPVLVGTISIEKSEALSNMLKSMGIPHNVLNAKQHER
                     EAEIIANAGKPGAITIATNMAGRGTDIVLGGAQKYKDSIEKWNEADPLIEEFKLAIKS
                     GKFENAAAIAERIEVPSRKKRAKDIVEEALAWSRNHEDVIQKGGLHILGTERHESRRI
                     DNQLRGRSGRQGDPGSSRFYLSLDDDLMRIFGGDNISKIMLRLGMEEGQEIEHKLVTS
                     SIARSQKRVESHNFDIRKHLLEYDDVMNKQRMFIYSLRDEILDENHNMGATIRKWIEE
                     LIESQILIHCDSNNPKTWNHEMMNEWLGNLSPDLRFDPTRYNKSAKVHLQLLEELSEK
                     AVEIYDRKAKSVGEIWIQIERDLFLQILDHRWKEHLYVMDHLRDGIWTESYGDKNPLT
                     EYKIQGFKIFDQMVDNLKFEVLNFLLKVEVTEASAQQSEEHEYQKVGTESTNVDLFFS
                     GNSEDTKRKVTSEISSSAGGTSGRKSSRRRKR"
     gene            2670..3524
                     /locus_tag="PK683_00150"
     CDS             2670..3524
                     /locus_tag="PK683_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH06880.1"
                     /translation="MKLKNVLPHIHAGSTSCRVCDGVGIVLTENAANSPYPVLTVCHC
                     AKENCFTCPSRGKPPYMFYSEESNKMIPCLCHEARIKMGYLEKKVAVSEIPERYKFKF
                     LENVDFRESEKGTMSVYAAHDWAADMVENYKTNHSLQGMYLYGNAGSGKTLFACTILN
                     ELIFRYNADCRYVKINSFFSDLRETYQRDSNTYGQETDVIKEYSEVDVLVIDDFGVQK
                     DSDWVQSKLYDLIDKRYEKKNLTLLTSNHNLKDWESKWEGRIYSRLCEMTKEIHLDCP
                     DYRQKFRR"
     gene            3524..3949
                     /gene="folK"
                     /locus_tag="PK683_00155"
     CDS             3524..3949
                     /gene="folK"
                     /locus_tag="PK683_00155"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNH06881.1"
                     /translation="MRTAFLALGSNLGDRENYLKKAVRIIENHNYIIIIKKSTILETK
                     ALDFTDQPDFLNRVLKIQTGLSPQELLDFCKKSETEIGRTPSEIPKGPREIDIDILDY
                     SNLVINTDNLTLPHPALRTRPFIRTILESMGEWESLKSE"
     gene            complement(4564..7431)
                     /locus_tag="PK683_00160"
     CDS             complement(4564..7431)
                     /locus_tag="PK683_00160"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH06882.1"
                     /translation="MLRFIQSRKKLSLWIFSALNFIFSCSVCLISFYRSGYVLTEAAD
                     SRLTVAANAVDFILTLEYHEKESIDSDEYSSLNQKLNRYANEADVSYLYSFSEKEGRF
                     YNTSSSVLFQDEIDRFKSSLMLEIKESEQTAAFARALQSGKTVFADFSGKWGNFRSVI
                     VPHISKNGRKYITGAEIKTDVISEQKTETLYDSVFYGFCLFILLLIIYYDIVYLEKFH
                     KIRNLLNSRKMPGAVSAAAAALSVLWGISFFKDEKLNIYKSADSRLYVSALSLKYILP
                     DDYHEKADSGDREMPTEYVRQSKVLKLYSERIHTEYLYTYMEKNGNYYYTSCSFTEAD
                     IKNGTVPKYLEEVDKGSRLINASSENSASFFDGKMLKWGNSRTAAVPVRNLNRKPFLA
                     ASDIKSLELDRAAFRSFFTAALFGISAFFFTVLILSYAAANFDSIKYFINIKYNRFIS
                     IKFKLIFMIGSIFSMTITVLSLLFLYNSKTILKNKTLDVCENFAVNISNIAREDLVLD
                     TAYEAANTVVGDLVRTKVEGLLETYILNVYGKYVVDYSGARLGEFTTEKEIKYLQEIT
                     LIDLQETVSPKNGRYILRITYPIYINYNNQSLKIGAAVFEFDRDAVYAPIYKMQKIVF
                     FFGILFLTVILALTYFLSGYITDPILSLSKAVQTVSDGNLDYKIEVQTNDEVGILSEK
                     FNVMSANLKISYSHLEEKVQERTAELNATLKLIQRDLALAEKIQSTALHSDLSLFKNL
                     EIAVRYIPMTEVGGDFYSISPLEKKVRVFLADATGHGVQAALIVMAIQGIYEGIKNFA
                     LPVNEVLDIFNREFVRRYGTINSYLSCVLMDIDTENERLMFASAGHPPGLLVRSDRQI
                     QLLERTGSLIGVRSNSIYTMDEFVFQKNEKIYVFTDGIFEEFMAEEEFGEDRLHLILK
                     EDQSPDMDTALDAVLNKLNDFLENTGPQDDITFIGIKFPEG"
     gene            complement(7433..>7773)
                     /locus_tag="PK683_00165"
     CDS             complement(7433..>7773)
                     /locus_tag="PK683_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06883.1"
                     /translation="LEAGKQVFPAIRKYLNSIRILESNAKSRNSLARIREKEIKNIPL
                     YIRTGKGYYEQRKYGQAEELFQNVLLADPDNKNAKEYLRLSADKKTAMLKYEECLKTS
                     DKKCHLLWTR"
BASE COUNT         2503 a         1572 c         1596 g         2102 t
ORIGIN      
        1 aagagactcc ttccgatcgt agcaaagata aacgccctgg aagacaaaat ttccgcaatg
       61 agcgacagcg aacttcgggc acagacagca aaatttaaag aactattgga aaaaaaacag
      121 actctcgacg acattctgcc ggaagcattt gcgacagtaa gagaagcctc taaaagaact
      181 atgggactca ggcattatga tgtacagctg atggggggaa ttgctcttca ctggggaaac
      241 atcgcagaga tgaaaaccgg ggaaggaaaa actctgactt ccacgcttgc tgtatatctg
      301 aattcattat ccggtctggg tgtacattta gtcacggtaa acgactatct ggccagaaga
      361 gatgccaact ggatgcgtcc tatttatgaa tttctcggaa tcacggtagg gatcattcag
      421 catgatatgg actacgaaga aagaaaggcg gcatacgggt gtgatataac gtatggaaca
      481 aataatgaat acggctttga ctatcttcgg gacaatatgg tggaaagaaa agagcacaag
      541 gttcagaaac accataactt tgcaattgtg gatgaagtgg attcaatcct gattgacgaa
      601 gcgagaactc ctctcatcat ttccggtcca gcagaagaaa gcacagacaa gtacatccga
      661 gtcaatgcaa ttattcctaa gctggtagag aaagaagact atgaagtgga tgagaaggcc
      721 agaaacgttc tgcttacaga aaatggagtt cttcattctg aaagacttct gggtgtggac
      781 aatctctatg ctcctgaaaa cgtagaactg gtacaccatg ttcatcaggc actgaaagct
      841 cacaagatct tccagaaaga cgtggactac gtagtacaaa ataatgaagt tatcattgta
      901 gatgaattca ctggacgtct gatggcagga agaagatatt ccgacggtct tcatcaggca
      961 ctggaagcaa aggaaagagt gacaattgca agagaatctc agacacttgc atccattaca
     1021 tttcagaact tcttccgtat ttacacaaaa ctttccggta tgacaggtac tgcagataca
     1081 gaagcagaag agtttgcaaa gatttacaaa ctggatgtaa tcgtcattcc accgaatctg
     1141 aagatgaaga ggatagacaa tccggacaga gtctacagaa ccggaaagga aaaattcaat
     1201 gcgatcgtaa atgaaatcaa ggactgttat acaagaaaac agccggttct tgtcggaacg
     1261 atctctatcg aaaaatcgga agcactttcg aatatgctga agagtatggg tataccccat
     1321 aatgtattaa atgcaaaaca gcatgaaaga gaagcagaaa ttattgcgaa cgcaggaaag
     1381 cctggagcca ttacgatcgc aacgaatatg gcgggaagag gaactgatat cgttctggga
     1441 ggagcacaga aatacaagga ttccatagaa aaatggaatg aggctgatcc tctgattgaa
     1501 gaattcaaac tggccataaa gtcaggcaag tttgaaaatg ctgctgcaat cgcagaacgc
     1561 atcgaagttc caagcagaaa gaaaagagca aaggacatcg ttgaagaagc actggcttgg
     1621 agcagaaatc atgaagatgt aattcaaaaa ggcggtcttc atattctcgg aacagaaaga
     1681 cacgagtcca gacgtatcga caatcagctt cgaggacgtt ccggacgcca gggagatccg
     1741 ggttcaagcc gtttttacct ttctctggat gatgatctga tgcgaatttt cggaggagac
     1801 aatatatcca aaattatgct ccgcctagga atggaagaag gccaggaaat cgagcataag
     1861 cttgtgacaa gttcgattgc aagatctcag aaaagggtgg aatcgcacaa ctttgatatc
     1921 agaaaacatc ttctggaata tgatgatgtg atgaacaagc agaggatgtt tatctattca
     1981 ttgagagatg aaattctgga tgaaaatcac aatatgggtg ctacaatccg caaatggatt
     2041 gaagaactca ttgaatctca gatactgatt cactgcgatt ccaataatcc gaaaacttgg
     2101 aatcatgaga tgatgaatga atggctcggc aatttgagcc ctgacctcag atttgatcca
     2161 acaagatata ataagtctgc aaaagttcat cttcagcttc tggaagaact gagtgaaaag
     2221 gctgttgaaa tttatgacag aaaagcaaag tccgtaggtg agatctggat tcagatcgaa
     2281 agagatcttt tcctgcagat tctggatcac agatggaaag aacatctgta cgttatggat
     2341 catctcagag acgggatctg gactgaaagt tatggagaca aaaatccgct gactgaatac
     2401 aagattcaag gattcaagat ttttgatcag atggtggaca atcttaagtt tgaagtgctg
     2461 aacttccttc tgaaagtgga agtgactgaa gcttctgctc agcagtcgga agaacatgaa
     2521 taccagaaag taggaactga gtctacaaat gtggatctgt tcttttccgg aaactcagaa
     2581 gacaccaaac gcaaagttac ctctgaaatt tcttccagtg caggcggaac ctccggaaga
     2641 aaatccagca gaagaagaaa acggtaacat tgaaactcaa aaatgttctg ccgcatatcc
     2701 acgcaggaag cacttcctgc agggtctgtg acggtgtagg aattgtgctg acagaaaatg
     2761 cagccaattc cccatacccg gttctgactg tctgccactg tgcaaaggaa aactgtttta
     2821 cctgtccttc caggggaaaa cctccctata tgttctattc ggaagaaagc aataaaatga
     2881 ttccctgtct ctgtcatgag gcaaggatca agatgggtta cctcgaaaag aaagtggcag
     2941 tttctgaaat ccctgaaaga tataaattca aatttttgga aaatgtagat ttcagagaat
     3001 ctgaaaaagg aactatgagc gtatatgcag cgcatgactg ggctgctgat atggtggaaa
     3061 actataagac taaccacagc cttcagggaa tgtacctgta tggaaatgca ggaagcggaa
     3121 aaactctttt tgcctgtaca attcttaatg aacttatatt ccgttataat gcagactgca
     3181 ggtatgtaaa aatcaacagt ttcttcagcg acttgaggga aacatatcag agagacagca
     3241 atacatacgg tcaggaaaca gatgtcatca aggagtattc agaagttgat gttctggtaa
     3301 tagatgattt cggtgttcag aaggattccg actgggttca gtccaagctg tatgatctga
     3361 tagacaaaag atatgaaaaa aagaatctca ctctgctcac gtccaatcat aatctgaagg
     3421 actgggaaag caaatgggaa ggacggattt attccaggct ctgcgagatg actaaggaaa
     3481 ttcatctgga ctgtccggac tacaggcaga aatttcgacg ctgatgcgga ctgcttttct
     3541 tgcactgggc tccaatctgg gagacagaga aaactatctt aaaaaagctg tcaggatcat
     3601 cgaaaatcat aattacataa taattataaa aaagtcaact atactggaaa caaaggcact
     3661 tgattttaca gatcagcctg atttcctgaa ccgagtttta aaaattcaga ccggcctctc
     3721 tcctcaagag cttctggatt tctgcaagaa gtctgaaacc gaaataggca gaactccatc
     3781 agagatccct aaaggtccaa gagaaatcga cattgatata ctggattatt caaatttagt
     3841 cataaacacg gataatctca ctcttccgca ccctgctctc cgcacaagac cgttcatcag
     3901 aacaattctg gaaagtatgg gtgaatggga aagtcttaag tcagaatgac aatttattat
     3961 tgatttctat tttataaaag atatactgtc atatatatta taccaagaaa atatgtatga
     4021 tttgttcatt tactgattct aatttccctc atatcggact gttcatcccc gaaaaacagc
     4081 aatgggaaaa catatcttcc tgctgcagcg gcattgattc tctctcagcc gtcagtctca
     4141 gtctcttacg aatgcagcgg tacatccgtt aaaacagaga gttctgtcag atcaccggaa
     4201 tcagaaaact ttactgtaaa ttctgaagat gcggactcaa caaatataaa aagtcatgga
     4261 agagaaaaag gaacagttta tcaacctgag cgatgactgt tattccggca ttgctgtgca
     4321 taaaatcggt catacagttg gactagcaca tgagcacaac agaacggaca gaaattcatt
     4381 cattctcata ctgcctgaaa atattcagtc aggacaggaa cagaacttta ctcaatcttc
     4441 agccagatat ttttcaaatt actgtctgat catttatctg aatctgctga cacacgaaga
     4501 actgacctca gaccgtttgg agattgagag ttgtttactg cattcaggaa tgcgacaaca
     4561 gtttcatccc tcaggaaatt ttatcccaat aaatgtgata tcatcctgcg ggccggtatt
     4621 ttccagaaaa tcattcagtt tattcagaac agcatccagt gcagtatcca tatcaggcga
     4681 ctgatcttcc ttcagaatca gatgaagtct gtcttctccg aactcttcct cagccataaa
     4741 ctcttcgaaa attccatctg taaaaacata aattttttca tttttttgaa atacaaactc
     4801 atccattgta tatattgaat tgcttcttac tccaatcaga gatccagtcc tttccagaag
     4861 ctgaatctgc ctgtcggatc ttaccagcag accaggagga tgtcctgcag acgcaaacat
     4921 cagtctttca ttttctgtgt caatatccat aagaacacag gacagatagg aattgattgt
     4981 gccgtacctc ctgacaaatt cacggttgaa aatatccaga acctcattta cgggaagcgc
     5041 aaaattctta attccttcgt atataccctg tattgccatt acgatgagtg cggcctgtac
     5101 gccgtgacca gttgcatcgg ccagaaaaac cctgaccttt ttttccaaag gactgatgct
     5161 gtaaaaatct cctccaacct cagtcatcgg aatgtaacgc actgcaattt ccaggttttt
     5221 aaataatgac agatcagaat gaagagctgt ggactgaatc ttttctgcaa gagccagatc
     5281 cctctgtatc agtttcagcg ttgcattcag ctccgcagtt ctttcctgca ctttctcttc
     5341 cagatgagaa tatgatatct tgagattggc actcataaca ttgaattttt cagacaggat
     5401 tccaacctca tcatttgtct gaacttcaat cttataatcc agattaccgt cggatactgt
     5461 ctgcacagcc ttggaaagag aaagtatggg atcagtgata taaccggata aaaaataagt
     5521 cagggcgaga attacagtca gaaacagaat cccgaaaaaa aacactatct tctgcatttt
     5581 ataaataggt gcataaacag cgtccctgtc gaattcaaac accgccgcac cgattttcag
     5641 tgattgattg ttataattaa tatatatagg ataagtgata cgaagtatat atctcccgtt
     5701 tttgggtgaa actgtctcct gaaggtcaat cagcgtaatt tcctgaagat acttaatctc
     5761 cttctccgtg gtaaactctc ccagtctggc accggagtag tcgacgacat attttccata
     5821 cacgttcaga atataagttt caagaaggcc ttccaccttt gttctgacca gatctcccac
     5881 aacagtgttt gcagcctcat atgcagtatc cagaaccaga tcttctctgg caatattgga
     5941 aatattcact gcaaaattct cgcagacatc cagagtctta ttcttgagta tcgtcttgga
     6001 attataaaga aaaagaagag aaaggactgt gatagtcatt gaaaatattg aacctatcat
     6061 aaatatcagc ttgaacttta tgctgatgaa cctgttatat tttatattta tgaaatattt
     6121 aattgaatca aaattcgccg ctgcataact taaaatcagc acagtgaaaa agaatgcaga
     6181 aatcccgaaa agagccgcag taaaaaaaga tctgaaagct gccctgtcaa gctccagaga
     6241 cttgatatcg gatgcggcca gaaacggttt ccgattcaga ttcctgacag gaaccgctgc
     6301 ggttctggag tttccccact tcagcatctt cccgtcaaaa aatgaggctg aattttctga
     6361 agaagcatta atgagccgac tgcctttatc cacttcttcc agatattttg gaacagtccc
     6421 gttttttatg tctgcttcag taaagctgca cgaagtgtag taataattcc cgttcttttc
     6481 catataagta tagagatatt cagtatgaat tctttcggaa tacagtttca gaaccttaga
     6541 ctgtctgaca tactctgtcg gcatttctct gtcccccgaa tcagcctttt catgatagtc
     6601 gtccggaaga atatatttta atgataaggc ggaaacataa agtctggaat cagcagactt
     6661 gtatatattc agtttctcat ccttaaaaaa ggaaataccc cataatacag aaagagcagc
     6721 tgcagctgct gaaactgcac caggcatttt tctgctgttc agaagatttc ttattttatg
     6781 aaatttctca agatatacaa tatcatagta tattatcagg agcagaataa aaagacagaa
     6841 tccgtaaaaa actgaatcat aaagggtttc cgttttttgt tccgaaatga catcagtctt
     6901 aatttctgca cctgtaatgt atttcctgcc gttctttgaa atatgcggaa caatcaccga
     6961 tctgaagttt ccccattttc ctgagaaatc ggcaaagact gtctttcctg actgaagtgc
     7021 cctggcaaag gcggcagtct gttcagactc tttgatttca agcataagac tggatttaaa
     7081 tctgtctatc tcatcctgaa acagaacact ggaagatgta ttgtaaaacc tcccctcctt
     7141 ttcagaaaaa gaatagagat aacttacgtc tgcttcattt gcatatctgt tcagtttctg
     7201 attcaaagaa ctgtattcat cagaatctat gctctctttt tcatgatact caagggtcag
     7261 aataaaatcc actgcatttg cagcaacagt gagacggctg tctgcagcct cagtcagcac
     7321 ataacctgat ctgtaaaagc tgataagaca gacagagcag ctgaatataa aattcagcgc
     7381 cgaaaagatc cagagtgaaa gcttttttct gctttgaatg aacctgagca tttcagcgtg
     7441 tccatagaag atgacatttt ttatcggacg tcttcaggca ttcctcgtac ttaagcatcg
     7501 ctgttttctt gtctgcagaa agtctgagat actcctttgc attcttattg tccggatctg
     7561 caagaagaac gttctgaaag agttcctctg cctgaccata ttttctctgc tcataatatc
     7621 cttttcctgt tctgatatac aatgggatat tttttatttc tttttccctg attcttgcca
     7681 gtgaattcct ggacttggca tttgattcaa gaattcggat ggaattcagg tattttcgga
     7741 tggcaggaaa aacctgcttt cctgcctcca gtc
//
LOCUS       DAOQGJ010000005         7244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_5025_length_7244_cov_2.818751, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000005 DAOQGJ010000000
VERSION     DAOQGJ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5025_length_7244_cov_2.818751"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..406)
                     /locus_tag="PK683_00170"
     CDS             complement(38..406)
                     /locus_tag="PK683_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH06884.1"
                     /translation="MRILVIDDTGAQRFIIKRLLQQECGIAASDIDEAENGEKGLEKT
                     AGSEYGLIIVDWNMPVLDGLEFLKEIRKNRIMTPVVMVTSENEESKKESAVAAGANHF
                     LTKPFTGSALLEILRNYISK"
     gene            complement(418..1224)
                     /locus_tag="PK683_00175"
     CDS             complement(418..1224)
                     /locus_tag="PK683_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNH06885.1"
                     /translation="MKNMYHFIRQFLYKNVGIVLEPEKDYIIEHRLSCAAKESGFSSL
                     KDFLRSAAEETDKYSDILIDELTTTETFFFRDKNYFDLLQKHIIPETIKKNMDEKKIQ
                     IWSAVCASGQEAYSIKIILDEYFSRLQDWKFQIYCSDINSRMLEKAKKGEYSEMEVGR
                     GLPIEYKGRYFHEYHDKYVIDRNLVKDMHFSRINLMDDSYNMPLMDIILLRNVLIYFD
                     EKTKNETVRKTAGRLKRGGYLLLGSTEYLKDYENDFRQIRISNTTAYQKI"
     gene            complement(1231..2289)
                     /locus_tag="PK683_00180"
     CDS             complement(1231..2289)
                     /locus_tag="PK683_00180"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNH06886.1"
                     /translation="MKKKIRVLVVEDSILIRKILSDVIDSDSELEICATAPNGKIALG
                     KIHYDNPDILTLDLEMPEMNGFDTLKEIRKLYPNLPVIILSSVNRSGASGTLECLQIG
                     ANDYVAKPEGFTERKDINEYLKDNLLPKLKHFGMGKEYSQSVRKKSPLIFLKNKSIDY
                     KNFDIICIGVSTGGTSVLNSIIPSVPEDFPLPIVIVLHIPPVFSKIISEQLNEKSRIR
                     VAEIADGEILENGKAYLAPGDFHASIRKKNDHTVFVLDAGEKVNFCRPSVDVFFKSAA
                     AVFGKRTLGIILTGMGHDGLDGARNIKERGGTVIVQDEESSVVWGMPGSVFKAGLADF
                     VMSDEEIVHLFSSRSHPE"
     gene            complement(2286..3566)
                     /locus_tag="PK683_00185"
     CDS             complement(2286..3566)
                     /locus_tag="PK683_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH06887.1"
                     /translation="MTAVQKKNSESKKKQNAENILESDFFRSILSFTGDLLIVHSNAG
                     KDVLYSAKLLELLNLKQSAESASDFSSFFEYVHKDDKALFEEEIKNYLSDKTYGKSFK
                     NQIRIKQNSDEYSTFSVTADSVGEEPERILLIRLNDETEKKHSEELEKRYVKKQETLI
                     AGAKEIVTELSTGKDKLVKFCENLTVNTQQAFKETETVSKSSNAVSSNVQTVAIATEE
                     MSSNISQIVKAVTEASKVAGKAVESAKEANDTILKLGDSGVAIGKVINVITSIAQQTR
                     LLALNATIEAARAGEAGKGFAVVATEVKELAKETAQATEDIRQKIEAIQLDTKNAVNA
                     IAEIKKIIDQIHAIQGLIANSVEEQSVTTEDIAKNMSDAAVGSANINTGIENVSKLTN
                     EISGNSKFLQEIAYEIAAISDTFNYFLSQGIYKT"
     gene            complement(3589..4008)
                     /locus_tag="PK683_00190"
     CDS             complement(3589..4008)
                     /locus_tag="PK683_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH06888.1"
                     /translation="MEKLYCTFYLDQLYYGISISTIQEIIQNQPILPIPLASENVDGL
                     INLRGHIISVLNLNRILSGTESESELRTLLIIYTSAGLVGLRIDDVHEIISLSEEKKE
                     PPPATLHGNLKAFISHTYKLPESFLLILDAESVAAVQ"
     gene            complement(4008..6134)
                     /locus_tag="PK683_00195"
     CDS             complement(4008..6134)
                     /locus_tag="PK683_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNH06889.1"
                     /translation="MEEEIQYFLVEGYEELFKYEASLLDLEKDPNNSDLLQSVFRSIH
                     SIKGTCGFLGFSKLQEIAHKGENLLSSLRDGKIKVNAYLISVLLELSSVIKENMKEIE
                     TTGKESVQDYSQFISRLSAILEISLNMIKTPEEHETAGKTVVSETESSVSTVRIEIRH
                     LDKLMNLVGELVLNRNQILETSGRIKDPALQSNTLKLNQLTTELQEGIMKTRLQPIKT
                     VWNQLHKVVRNLSLELGKKARLEFQGEDTELDKSILEAVKDPLMHIIRNCMDHGIETP
                     EKRKQNGKNEEGHILLKAFHEGGQVSMQISDDGEGINVQKIREKAFFNGLITKEQKSE
                     MNDSDLLNLIFLPGFSTSETVTNVSGRGVGMDVVKSNLQKIGGTVNITNFPGKGILFK
                     ITIPLTLTIIPALIINCGGYKFAIPQTSLLEIVELSSEKLNTEIRKIHESYVYHLREE
                     FLTVIFLDELLKLPEKKQNSVSSIIIVQSENQKFGIIVDEALDTEEIVVKPLGKHVQN
                     IFAYSGVTILGDGKIALILNIQNILENIFTNIRNKSADELDQEQTKEENSSYVIFEVS
                     GNYRLALPLNSVLRIEEINPAHLKTSAGKKVLSYRDKFIPTIWLSGLLLGRDPEIPET
                     GIINMLIYQDKGYTIGFIISNIIDIAEGNYRIYPKDEAWKDMILERSILGSILYENES
                     VDVLDAEELIQEYKKRISDEVLSMVH"
     gene            complement(6451..6840)
                     /locus_tag="PK683_00200"
     CDS             complement(6451..6840)
                     /locus_tag="PK683_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06890.1"
                     /translation="MNYLDKDFAKVYDDGKHKDTVVLEWGEIFQTKAFIQEAVNAVLD
                     AVKTQKKTKLLALIKAKSYENSGLEWLVAEWYTTAYAAGLKKIAHLMGAKALAEIAAE
                     KVSTMDKSGMLFFNTSNVDEAYTWLNS"
BASE COUNT         1775 a         1554 c         1383 g         2532 t
ORIGIN      
        1 ctctttgagc tttcagcgga ggcaaaaaaa actgttttca ttttgaaata tagtttctga
       61 gaatttccag aagagcagaa cctgtaaacg gtttcgtcag gaagtgattc gcaccggctg
      121 caaccgccga ttcctttttg gattcttcat tttctgaagt gaccatgact acgggcgtca
      181 ttattctgtt ctttctgatc tccttcaaaa attcgagacc gtccaaaaca ggcatattcc
      241 agtccacgat aatcagaccg tattccgaac ctgcagtttt ttccaggccc ttttctccgt
      301 tctcagcctc atcaatgtct gaagcggcaa ttccgcactc ctgctgcaga agtcttttga
      361 taatgaacct ctgtgctcct gtatcatcta tgacaagtat tctcatattc tcactcctta
      421 gatcttctga taggcagtag tgttgctgat tctgatctgc ctgaaatcgt tttcatagtc
      481 ttttaaatac tcagtggaac cgagcagaag atatccgcct cgtttcagtc tgcctgccgt
      541 ttttctaacc gtttcatttt ttgtcttttc atcaaagtaa atcagtacat tacgaagcag
      601 gatgatgtcc atcagcggca tattgtagct gtcatccatc aggtttattc tgctgaaatg
      661 catatctttt acaagatttc tgtctattac atatttgtca tgatattcat gaaaatatct
      721 gcctttatat tcaatcggaa gtcctctgcc cacttccatt tcggaatact ctcctttttt
      781 tgccttttcc agcattcttg aatttatgtc ggagcagtat atctgaaatt tccagtcctg
      841 aagtctgctg aagtattcat cgaggatgat tttaatgctg tatgcctcct gtcctgaagc
      901 gcacacagcc gaccagatct gtattttttt ttcatccata ttttttttta tagtttccgg
      961 gataatatgc ttctgcagaa gatcaaaata atttttgtct ctgaaaaaga aggtttctgt
     1021 tgtagtcagt tcatcaatca gaatatcact gtacttatca gtttcttctg ctgcagatcg
     1081 caggaaatcc ttgagtgagc tgaatccgga ctcctttgct gcacaggaca gtcggtgctc
     1141 tatgatatag tctttttccg gttccaggac tattccgaca tttttgtaaa gaaactgccg
     1201 aataaaatga tacatatttt tcattattat ttattccgga tgcgatcttg aggaaaacag
     1261 atgtactatt tcctcatctg acattacaaa atcggcgagt cctgccttga acactgatcc
     1321 tggcattccc catacgacgc tgctttcctc gtcctgaacg atgacagtgc cgcccctttc
     1381 cttgatgttc cttgcaccgt ccagtccgtc atgccccatc ccagtcagaa tgattcccaa
     1441 tgttcttttt ccaaatacag ctgcggctga tttgaagaat acatccacag aaggtctgca
     1501 gaaattcact ttttctcctg catcgagaac aaaaaccgta tggtcatttt tcttccgtat
     1561 ggaagcatga aagtcgcctg gtgcaagata cgcctttccg ttttccagaa tctccccgtc
     1621 ggcaatctct gctactctga ttcttgattt ttcattcagc tgctctgata tgattttgga
     1681 gaaaacagga gggatatgca gaacaattac gatcggaaga gggaagtctt ccggaacaga
     1741 gggaattatg ctgttcaata ctgatgttcc gcctgtcgat acacctatgc atataatatc
     1801 aaaattttta taatcaatgg acttgttttt cagaaagatt agaggggatt tttttcttac
     1861 agactgactg tattcctttc ccattccgaa atgcttaagt ttgggaagaa gattgtcctt
     1921 aagatattcg ttgatgtctt ttcgctccgt aaatccttcc ggttttgcga cgtagtcatt
     1981 tgctccaatc tgcagacatt caagggttcc tgaagcaccg cttctgttga ctgaactcag
     2041 aataatcaca ggaaggttcg gatacagttt tctgatttcc ttcagcgtat cgaatccgtt
     2101 catttccggc atttccagat caagggtcag tatatccgga ttgtcatagt gtatttttcc
     2161 taaagcaatt tttccgttgg gggcagttgc gcatatttcg agttcactgt cggaatcaat
     2221 gacatctgaa aggatttttc tgatgagaat tgaatcctcc acaaccagaa ctctgatttt
     2281 ttttttcatg tcttgtatat gccctgtgac aggaaataat tgaaagtatc gctgattgca
     2341 gcgatttcgt atgcaatttc ctggaggaac ttggaatttc ctgatatttc gttcgtaagt
     2401 tttgaaacat tttctatccc ggtgttgata ttggcgctgc cgacagcagc atccgacata
     2461 ttctttgcaa tgtcctccgt tgtaacggac tgttcttcga ctgaatttgc tatcagcccc
     2521 tgaatagcat gaatctgatc aataattttt tttatttctg caatcgcatt cactgcattt
     2581 tttgtatcca gctgtatcgc ttcaattttc tgacgtatgt cttcagtcgc ctgagctgtt
     2641 tcctttgcaa gttccttgac ttcagtcgcg acgactgcaa acccttttcc tgcctcgcct
     2701 gctcttgctg cttcaattgt tgcattcagt gcaagcagtc ttgtctgctg agcaattgat
     2761 gtgatcacat ttatgacttt tcctatcgca accccgctgt ctccgagttt cagaattgta
     2821 tcattggctt cctttgctga ttcaacagcc ttgcccgcta ctttggatgc ttccgtaaca
     2881 gcctttacta tctggctgat attgctgctc atttcttcag ttgcaattgc cactgtctga
     2941 acattggatg agacagcgtt ggatgatttt gatacagtct ctgtttcctt gaacgcctgc
     3001 tgtgtattta cagtgagatt ttcgcagaac ttgacaagct tgtcctttcc ggtggaaagc
     3061 tcagtgacaa tttccttggc tcctgcgatc agagtttcct gttttttgac gtagcgtttc
     3121 tccagttcct cagaatgttt tttttccgtt tcgtcattga gtcttataag aagtattctt
     3181 tccggttctt ccccgacaga gtcggctgta actgaaaatg tgctgtattc atccgagttt
     3241 tgttttattc ttatctgatt tttgaatgat tttccgtatg ttttatcgga aagataattc
     3301 tttatttcct cctcaaacaa tgctttatca tccttatgaa cgtattcaaa aaatgagctg
     3361 aaatccgaag cggattccgc agattgtttc agattcagca gctcgagcag tttcgctgaa
     3421 taaagaacat ctttgcctgc attgctgtga acaatcagga gatctcctgt gaaactcagt
     3481 attgaccgaa aaaaatccga ttccagaatg ttttctgcat tctgtttctt tttgctttca
     3541 ctgttttttt tctgaactgc tgtcattttt gtaatccttc tgattgtatt attgtaccgc
     3601 tgcaactgat tctgcatcca gaatcagaag aaacgattcc gggagtttgt atgtatgcga
     3661 gatgaatgct ttcaggtttc cgtgaagtgt ggcgggcgga ggctcttttt tttcttccga
     3721 cagagatatg atttcatgga catcatctat tctcagaccg accagtcctg ctgaggtgta
     3781 tatgatcaga agtgttctca gctctgattc agactctgtt ccggaaagaa tcctgttaag
     3841 attgagaaca ctgataatat gtcctcttaa attaatcagt ccatcaacat tttctgaggc
     3901 cagcgggata ggaagtatcg gctggttctg aataatttcc tgaattgtgc tgatgcttat
     3961 tccgtaatac agctgatcaa ggtaaaaggt gcagtaaagc ttttccatca gtgcaccatt
     4021 gacagaactt cgtctgaaat tctttttttg tattcctgta tcagttcttc tgcatccagt
     4081 acgtctactg attcattttc ataaagaatg gatccaagaa tgcttctctc aaggatcata
     4141 tccttccatg cctcatcctt gggatatatt ctgtagtttc cttctgctat atctataata
     4201 ttgcttatta taaatcctat tgtataccct ttgtcctggt aaataagcat atttattatt
     4261 cctgtttcag gaatttcggg atctcttccc agaagcagtc cagaaagcca tattgtcggt
     4321 atgaatttgt ctctgtaaga aagaaccttt ttaccggcag atgtcttcag atgagcggga
     4381 tttatttcct ctatccttaa aactgaattt aaaggaaggg caagtctgta gtttcctgag
     4441 acttcaaaaa tgacatagga tgagttttcc tcctttgtct gttcctgatc cagctcatct
     4501 gcggatttat tcctgatatt ggtaaagatg ttttccagga tattctggat gttcaggatc
     4561 agtgctattt ttccgtctcc cagtattgta acacctgaat aggcaaatat gttctgtaca
     4621 tgtttgccca ggggtttcac tacgatttct tccgtatcga gtgcctcatc gacgataata
     4681 ccgaacttct gattttctga ctgaactata atgattgaac tgactgagtt ctgctttttt
     4741 tcaggaagtt tcagaagctc gtccaggaaa atgactgtca gaaattcttc ccgaagatgg
     4801 tagacatatg attcatgaat tttcctgatc tcggtattca gtttttcgga actcagttcc
     4861 actatttcca gaagacttgt ctgaggtatt gcaaacttat atccgccgca gtttattata
     4921 agtgcaggga taattgtaag agtgagagga attgtaattt tgaaaagaat accttttccg
     4981 gggaaattgg tgatgtttac tgtcccgcct attttctgaa gattggattt gaccacatcc
     5041 attccgacgc ctcttccgga aacatttgtc accgtttcgg atgtagaaaa acccggaaga
     5101 aaaatgagat tgagcagatc agaatcattc atttcagatt tctgctcctt tgtgatcagt
     5161 ccgttgaaaa aagctttttc cctgatcttc tgaacattga ttccctcacc gtcatcggag
     5221 atctgcatac tcacctgacc gccctcatga aacgccttga gaaggatatg tccctcttca
     5281 ttctttccgt tctgctttct tttttcagga gtttcaattc cgtgatccat acagtttctt
     5341 attatatgca ttaatggatc cttgactgct tcaagaatgg acttgtcgag ttcagtgtct
     5401 tcaccctgaa attccagtcg ggcttttttt cccagttcca gagacagatt tcttaccacc
     5461 ttgtgaagct gattccatac tgtcttgatc ggctgaagcc tggtcttcat tatgccttcc
     5521 tgaagttcag ttgtcagctg attgagtttg agagtattgc tctgcagggc aggatctttt
     5581 attcttccag aagtttccag tatctgattt ctgttcagga caagttcgcc gacaagattc
     5641 atcagtttat ccagatgacg aatttcaatt ctgacagtgg atactgaact ttccgtttcg
     5701 gagacaactg ttttacctgc agtttcatgt tcttccggtg tcttgatcat attcagactg
     5761 atttccagaa tcgcagacag cctgctgatg aactgtgagt agtcctgaac actttctttg
     5821 ccagtcgttt cgatttcctt catattttct ttaatgacgg aggagagttc cagaagaacg
     5881 gagatcagat aggcattgac tttgattttc ccgtctctga gggaagagag gagattctct
     5941 cccttatggg ctatctcctg aagtttgctg aaaccaagaa acccgcaggt tcctttgatg
     6001 ctgtggatgg atctgaatac tgactgaagc aggtctgaat tgtttggatc tttttcgaga
     6061 tctagaaggc ttgcctcata tttgaaaagt tcctcgtagc cttctactaa aaagtactga
     6121 atctcttctt ccattttccc ttttcaaaac cttgactgtc tcaatgcgat gaattcacct
     6181 ttggcaggcg agacagttct tgagataaaa aactgttctg tatgtgccta aagtcaaaat
     6241 tcattcacct tttctttata tactgctgaa tatcagtatt ttaaggataa tacagcaaac
     6301 aagacaaaaa tgtaaatcta ttctttttag aataattttt tttgtaaatc caatttttgc
     6361 attgcgcact gcaatataaa tttttttaca atttttcgga actgaggcgg tttccctgcg
     6421 tcagttccga aggtcaacca gtcaattcgg tcaggagttc agccaagtgt aggcttcgtc
     6481 aacattgctg gtgttgaaaa acagcattcc ggacttgtcc attgttgaca ccttttctgc
     6541 tgcaatttct gccagcgcct ttgcccccat caggtgtgca atttttttca ggccggctgc
     6601 gtatgcagtt gtgtaccatt ccgccaccag ccattccagg cctgaatttt cataactttt
     6661 ggccttgatg agtgccagca gctttgtctt cttctgagtt ttgactgcat ccaatacagc
     6721 gttcacagct tcctgaataa atgcctttgt ctgaaatatt tccccccatt ccagaactac
     6781 agtatctttg tgtttcccgt catcgtacac ttttgcaaaa tctttatcaa gataattcat
     6841 tttttgctcc ttaatttgag ttttgtcgga tatgattaag catattaacg gttttttgcc
     6901 tcgggaaata caggagtaaa tatttttttt atgaatcggc ttttggattc atcctgctgc
     6961 ggacagtttt cttttctgcg tatgcagata tattcctttc ataaaacaga cagttctgta
     7021 tatctcagat ttctaatctc gcaagtatgg atgccgctgc tgcgctcgat tagaaatctg
     7081 ggtatatgta ttcatttaag atcgctgaat agagggaatt ctcctgtttc tttctgactg
     7141 agaattctat tttttcagaa atatttgaaa aactagaatt ataaatttat tatattaaaa
     7201 aaatatttac tagtttacct tgttcgaaaa tgatctgctg tccg
//
LOCUS       DAOQGJ010000006         6906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_5346_length_6906_cov_3.492191, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000006 DAOQGJ010000000
VERSION     DAOQGJ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5346_length_6906_cov_3.492191"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PK683_00205"
     CDS             <1..774
                     /locus_tag="PK683_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNH06891.1"
                     /translation="VPVYWTFSGWNSPLALGEEIENPQKTIPRAMILGPIFVTLIYML
                     FCLVFVAVLSYSSFRTGSSDPYFDIGKFLLHNLGMLDTAGHDSISKGISLILFFIILG
                     NTNSAMITGSRIYVAMARDKLFWQKVGYVDPVKGTPVVSIWLQSFWAIILLLTMDKES
                     NLLNFAFMAITLLSAMTIFSLFIARKKLKTKGSFQAFGYPFTPIFYIVSSLGIMILVA
                     IGYIREGKYFVLYASFLCVAAALIAYEIWKSVMIKEERK"
     gene            771..2261
                     /locus_tag="PK683_00210"
     CDS             771..2261
                     /locus_tag="PK683_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNH06892.1"
                     /translation="MRFTVFFAVFIFSVFSADRIPTESLYEATDMVPVKIQRKHLDSL
                     REFSRAEGFKDPVYTFRIKNTAVVLVQKSSLSKLDSEGIPYQKSQSYSPFRYYDPTYS
                     ERFRKGFAELSDLKTGYKDHKLNEIYLRSLARAFPEEVTYFEIGKTRLGRPIPAIRIT
                     SNNGDESKPSVLFNGAHHSNELIGTEHCYDIIFHLLNEKNHYKKYLDRMNIWFIPIVN
                     PDGSWLFWYKSFAMGRKNGYLAPSHKETDIRRGVDINRNYPFKWNSGHPTASSGDINH
                     PFYRGSSPASEPETKAMMEIAESERFLLSMSFHSFAGKILFPYSIEDTVNPIPDYPGG
                     LAKRLVSVARSFSGRNYQAVKNLYPVDGTDQDYFFNKHGTVALLTESTHRNIEFANVE
                     KVLSGFRPVWKKFLEEYFTANKLILRITDREKEPVEAQITIDELEFYEGEELRNNPEN
                     GYFHRLMPDDREYTVRIKNVNFQEELVRVRAKKDFTPVSVILKKKK"
     gene            2258..4309
                     /locus_tag="PK683_00215"
     CDS             2258..4309
                     /locus_tag="PK683_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNH06893.1"
                     /translation="MKHLLQKVRRRKFIFSLAYISCFFLLIRILHFAFPAESKVFQSV
                     ILAQFFISFAAFLYSLKVGRIVTVVGAISAVRMESLAFSSPGLVTNAGIFAGASAGIL
                     FRYLFFEKKSSSTQSLKYSFTSTQLYGSLIFFLSVLLLDRFIVLFNAPFFRGMGIQET
                     MYFPSLSSHSAFSISAGLGLGLIFPLLYFMQEESNITDRKSGAELSAHFRAGLWAALL
                     FNAPVVLIQTYFNVNFLASGTNNAYQYERVTGLFRDAGSASWIFPVLILFFYWHLAFY
                     GRKFQRTSRIFSGVKKESFIFILSFAVLIILLVFGIRQGRAYWLISGAGYPVITYLIY
                     RKRNKKIILALVLFCAVSLPAVLTLPTMSRLRNSLNSVLSSGSLIKAAEAADSERFFL
                     NRAALEVFYDSPVFGNGLSSLIPNLFNPDFPIRHPSGIIDNPGMILTGILSDTGLLGL
                     LTLLIWISMHSYSRKRFAGLVFLLIPSLTGYQAVHPDGAFLYLMLIGPVYKICRYNRY
                     IVFSGSIFIFLIGACYAGNIFFRVNHQNWGPEFRLNHILSYQLFPFQMNLAAEKNRLL
                     PERKDLPPSISFHHFKGKLIWKLGRKEKIVPYAFLSDGTRKNILQQKWTFLDKQENEI
                     ISHRLILEKWKIRGEELKVPVHAVYIQVEELDSEGRILTYGDTVFAVLSIQFTKLNEV
                     E"
     gene            complement(4361..4705)
                     /locus_tag="PK683_00220"
     CDS             complement(4361..4705)
                     /locus_tag="PK683_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNH06894.1"
                     /translation="MKLIIAIIQPSKLEEVKAELTKNEIYRLTVSDVQGYGQQKGKTA
                     IYRGHEYQVNLLPKVRLEIAVNDNFVKPTIDAILKAAKTGEGQIGDGKIFILPIEDVI
                     RIRTGERGSSAI"
     gene            complement(4718..5980)
                     /locus_tag="PK683_00225"
     CDS             complement(4718..5980)
                     /locus_tag="PK683_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNH06895.1"
                     /translation="MIIFGQAATTPTPAPATPPAPVLDKADTVWMIVSSALVFFMIPG
                     LALFYGGIVRSKNVLSTMMHSFIAIIALTLQWTIFGYSFSFSGTNPFFGDFQMAFLSG
                     IDENTLEGTIPKFVHFLFQGMFALITPALISGAIAERVKLSGYVAFILLWATFVYDPV
                     AHWVWAADGWLFKKGALDFAGGTVVHLISGIAGLAAAIVIGKRKGDAESGSLTNPNNM
                     TYTLIGSGLLMFGWFGFNAGSGLAVNGVAARAFLVTFIAPAAAGASWLILEFLHTKKA
                     TALGAASGIVAGLVVITPASGFVGVTGALIMGLLVSPICYGAIMMKGKLGYDDTLDAF
                     GIHGVGGAAGAILTGVFTLSLGAGVASKGDQIMVQIISVLATGAYSFIISYALAFAID
                     KTIGFRIEEKKEINGLDEEIHGEKGYSI"
     gene            complement(6399..>6906)
                     /locus_tag="PK683_00230"
     CDS             complement(6399..>6906)
                     /locus_tag="PK683_00230"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH06896.1"
                     /translation="SVQDYRDICTKDPERYKKTELFRGMIIEKMTKSAKYDFFSQILF
                     EEFNGILPSGYILRQEKGIQIQDSELEPDISIVQGSAKDFKFSKPTTARLIIEISVSS
                     PVYDRNKAMDYSIANAEEYWILDAENEKLEVYTEPGKNGYKKKEILTDSDTLEIFGKK
                     IELKKIFE"
BASE COUNT         1830 a         1511 c         1424 g         2141 t
ORIGIN      
        1 gttccggtgt actggacctt ttccggatgg aactctcctc ttgctctcgg cgaggaaata
       61 gagaatccgc agaagactat ccctcgtgcc atgattctcg ggccgatttt cgtcacactg
      121 atctatatgc ttttctgtct tgtctttgtt gcagttcttt cctattcaag tttcagaaca
      181 ggcagttctg atccttattt cgacataggc aaattccttc tgcataatct gggtatgctg
      241 gatactgcag gacacgattc gataagcaaa gggatctctc tgatactttt ctttattatc
      301 ctgggaaata caaattcagc aatgattaca ggaagcagga tttacgtggc aatggcaagg
      361 gacaagctgt tctggcagaa agtcggatat gtggatccag tcaagggaac tcctgtggtc
      421 agcatctggc ttcagagctt ctgggcgatc atcctgcttc tgacaatgga caaggaaagc
      481 aatctgctga actttgcatt tatggcaatc actcttctgt ccgcaatgac catcttctcg
      541 ctttttattg cgcgcaaaaa actcaagacg aaaggttcct ttcaggcgtt cggatatcct
      601 ttcactccaa tattttatat agtcagttcg cttggcatca tgattcttgt cgccatcggc
      661 tatatcagag aaggaaaata ctttgtgctg tatgcatcgt ttctgtgtgt cgcggcagct
      721 ctgattgctt atgaaatctg gaaatcggtg atgataaaag aagaaagaaa atgagattca
      781 cagttttttt tgccgtattc atattttcag tcttttcagc agacaggata cctacggaaa
      841 gcctttatga ggcgacagat atggttcctg tcaaaattca gagaaaacat ctggacagtc
      901 tcagagaatt cagcagggca gaaggattca aggaccctgt atacacattt cgaatcaaga
      961 atacggctgt cgttctcgtg cagaaatctt cactttccaa gctggactca gagggcattc
     1021 cttatcagaa gtctcagtct tattctccct tcagatatta tgaccctact tattcagaac
     1081 gcttcaggaa aggattcgcc gaactctctg atcttaagac aggatacaaa gatcataagc
     1141 tgaatgagat ttatctaagg tctcttgcaa gagcatttcc ggaagaggtg acatactttg
     1201 agatcggaaa gacaaggctg ggcagaccga ttcctgcgat aaggatcact tccaacaacg
     1261 gagatgaaag caagccctct gtccttttca acggagcaca tcattcaaat gaactcatcg
     1321 gcacagaaca ctgctatgac attatttttc atcttttgaa tgaaaaaaat cattataaaa
     1381 aatacctgga caggatgaat atctggttta ttcccattgt aaatcctgac gggagctggc
     1441 ttttctggta caagagcttt gcgatgggaa ggaaaaacgg atatctcgca ccttctcaca
     1501 aggagactga cataagaagg ggagtggaca tcaacagaaa ttatcctttc aaatggaatt
     1561 caggacatcc gactgcatct tcaggggata tcaatcatcc cttctacaga gggtcctctc
     1621 ctgcttctga gcctgagaca aaggcgatga tggaaattgc cgaatcggaa agatttcttc
     1681 tttccatgag ttttcattct tttgccggga agattctttt tccatattcg atagaagata
     1741 cagtcaatcc gattccggat tatccgggag gacttgccaa aagacttgtt tctgtcgcaa
     1801 gatcattcag cggaagaaat tatcaggctg taaagaatct gtatcctgtg gacggaactg
     1861 atcaggacta ttttttcaat aagcacggca ctgttgcact tctcactgaa agcactcaca
     1921 gaaatattga atttgccaat gtagaaaaag tgctttcggg ctttcgtccg gtatggaaaa
     1981 aatttcttga agaatacttt actgcaaata agctgattct tcgaatcact gaccgtgaaa
     2041 aagaacctgt cgaagcacag atcacgattg atgaactcga attttacgag ggagaagaac
     2101 tcagaaacaa tcctgaaaac ggttattttc acagacttat gcctgatgac agagaatata
     2161 ctgtccgcat aaagaacgtc aattttcagg aagaacttgt ccgagtaaga gcaaagaagg
     2221 actttacgcc tgtttctgtc atactgaaaa aaaagaaatg aaacatctgc ttcagaaagt
     2281 gcgccggagg aaattcattt tttctctggc ttacatttcc tgtttttttt tactgatccg
     2341 cattctgcat tttgcgtttc cggctgaatc gaaagtattc cagtcggtca tactcgctca
     2401 gtttttcatc agcttcgccg ccttccttta ttcactgaaa gtcggtcgaa ttgtgacggt
     2461 ggtcggggcg atttctgcag taagaatgga atcgcttgca ttctcctctc ccgggctggt
     2521 gacaaatgca ggaatttttg caggggcatc tgccggaatt ctgtttcgtt atcttttttt
     2581 tgaaaagaaa agttcctcaa ctcaaagcct gaaatattca ttcacttcga ctcagcttta
     2641 tggaagtctg attttttttc tctctgtgct tctcctggac agattcattg tcctttttaa
     2701 tgcaccgttt ttccgaggaa tgggaattca ggagacaatg tatttcccga gtctctcctc
     2761 acattcggcc ttttcgattt ctgcaggtct tggacttgga ctgatttttc ctctgctgta
     2821 ctttatgcag gaggagtcca atatcactga cagaaaatca ggagcggagc tttctgccca
     2881 tttcagagca ggtctctggg cggcccttct gttcaatgct cccgtcgttc tgattcagac
     2941 ttatttcaat gtcaatttcc tggcatcagg aaccaataat gcatatcagt acgaaagagt
     3001 gactgggctt ttcagggatg ctggttcagc ttcatggatt tttccggttc tgattttatt
     3061 cttctattgg catctggcct tctacggaag aaagtttcaa aggacctcac gtattttctc
     3121 cggcgttaaa aaagaaagct ttattttcat tctttcattt gccgttctca taatcctgct
     3181 tgtgttcgga atccgtcagg gcagggcata ctggctcatc tctggtgcgg gatatcctgt
     3241 aattacgtat ctgatttaca gaaagagaaa caagaaaatc atccttgcac tggttctttt
     3301 ctgtgccgtc agtcttcctg ctgtactgac tctgcctaca atgtcccgtc tcaggaattc
     3361 tttaaattct gtcttgagtt caggatctct gataaaggcg gctgaagctg cagattcaga
     3421 gcggtttttt ctgaacagag ctgctctgga agtcttttat gattcccctg ttttcggaaa
     3481 cgggctttcc tccctgattc caaatctgtt caatcccgat tttcctattc gtcacccttc
     3541 agggatcata gacaatcctg gaatgattct gacaggtatt ctttcggata cagggcttct
     3601 gggacttctg actctgctga tctggatttc aatgcattcc tattccagaa agcgatttgc
     3661 cggccttgtc tttcttctga ttccttctct tacaggatat caggctgttc atcctgatgg
     3721 tgcatttctt tatctgatgc tcatcggtcc tgtatataaa atatgcagat acaatcgtta
     3781 tattgtgttt tccggttcca tttttatttt tctgatcggc gcctgttatg caggtaatat
     3841 atttttcaga gttaaccatc agaactgggg tcctgaattc agactgaatc atatactttc
     3901 ttatcagctt ttcccttttc agatgaatct tgcagcagaa aagaacagac ttctccctga
     3961 acgcaaggac cttccgcctt ccatctcatt tcatcatttc aaagggaagc tgatctggaa
     4021 actcggcaga aaggaaaaga ttgttccata tgcatttctt tcggacggaa cgagaaagaa
     4081 cattcttcag cagaaatgga catttcttga caagcaggag aatgaaatta tatctcacag
     4141 acttattctg gaaaaatgga aaattcgcgg ggaggaactg aaagtccctg ttcatgctgt
     4201 gtatattcag gtggaagagc tggattcaga aggcaggatc ctgacgtatg gagatactgt
     4261 atttgcagtg ctttccattc agtttacaaa gctgaatgaa gtggagtagt tatacttatc
     4321 aaaactgccg tatgcacatc gtgcatacgg cagttttgta ttaaattgcg ctggaacctc
     4381 tttcaccggt tctgatgcgg attacatctt cgatagggag gatgaatatt tttccgtctc
     4441 cgatctgtcc ttcgcctgtt ttcgcagcct tcagaattgc atctattgtc ggctttacga
     4501 aattgtcgtt cactgcaatt tccagacgaa ccttcggaag aagattcacc tgatactcat
     4561 ggcctctgta gattgcagtt tttcctttct gctgaccgta tccctgcaca tcacttactg
     4621 tgagacggta tatctcattc ttggtaagtt ccgccttgac ctcttccaat ttgctcggct
     4681 gtataatcgc tataatcagt ttcataattc ccccttatta tatactgtaa cctttttcac
     4741 catggatctc ttcgtcgaga ccgttgattt ccttcttctc ttcaatacgg aaccctattg
     4801 tcttgtcaat tgcgaatgca agagcatagg agataatgaa agaatatgcg ccagtagcga
     4861 gaacactgat aatttgaacc attatctgat cgcctttgct tgcaacacct gctcccagtg
     4921 aaagagtaaa tacaccggtc agtatggctc cggcagcacc gcctacaccg tgaataccga
     4981 atgcatccag tgtatcatcg tagccaagtt ttcctttcat cataatcgca ccgtaacaga
     5041 taggagatac caaaagtccc ataatcagtg ctccagtcac tccgacaaat ccggaagcag
     5101 gagtaattac tacaagtcct gccactatgc ctgatgcggc accgagtgca gttgcttttt
     5161 tagtgtgcag aaattccaga ataagccatg aagctcctgc agcagcaggt gcaatgaatg
     5221 ttaccaaaaa tgctcttgca gctacaccgt ttacagcaag accggaacca gcgttaaatc
     5281 cgaaccagcc gaacatcaga agaccggaac cgatcagagt atacgtcata ttgttaggat
     5341 ttgtcagtga acctgattca gcatcaccct ttctttttcc gatgactatt gccgcagcaa
     5401 gccctgcaat ccctgaaatc aggtgaacta ctgttccccc tgcaaagtca agagctcctt
     5461 ttttgaaaag ccatccgtct gctgcccaga cccaatgcgc gacaggatca tatacgaagg
     5521 ttgcccaaag cagtataaat gctacataac cggacagttt cactctttca gcaattgctc
     5581 cggaaatcag tgcaggagta atcagagcaa acattccctg aaaaaggaag tggacgaact
     5641 ttggaatcgt gccttccagg gtgttttcat caatccccga gaggaatgcc atctggaaat
     5701 caccaaaaaa aggatttgtt ccagagaaag aaaaactgta cccgaatatc gtccattgca
     5761 gtgtaagtgc aatgattgct ataaaactgt gcatcattgt agaaagcaca ttttttgagc
     5821 gtacaatacc gccgtaaaac aatgccagac ctggaatcat aaaaaatacc agagctgaag
     5881 atacaatcat ccatacagtg tctgccttat ccagaacagg agccggcgga gttgccggtg
     5941 ccggagtcgg tgttgtggca gcttgtccaa atataatcat aggaaggaaa aatcctagga
     6001 ttgccgctgt tcgaaaaatg ttctttctag ttaacatatt gccctcactt tagcttatct
     6061 aagtgttatc gtggttttaa aagcaaatag cgtgccaagt tctctttctc tccttttatt
     6121 gaaggtgttc tgagaaaaaa agctaaaaat cgccggagct taatttacta ctctgctttt
     6181 ttttggctct gattgtctgt tttttgggct gaattggact atcgacttca gtaatacttg
     6241 tttttaaagc tttttgactg tttaagtcaa gggatttaga aaggcgtcca ttgttttaga
     6301 tcgttttttt gagtgcttat tgagctgtct ggtgataaaa tcagcaatct caattttacc
     6361 ggttgggcag ccttttcctt tttttgtgca gttctgcttc attcaaagat tttcttgagt
     6421 tcaattttct ttccgaatat ttccaaagta tcagaatcag tcagtatttc tttttttttg
     6481 taaccgttct tcccaggttc agtgtacact tccagctttt cattttctgc atccagtatc
     6541 caatactctt ctgcatttgc aattgaatag tccattgctt tattcctgtc gtaaaccggt
     6601 gaactgaccg aaatttcaat gatcaatctt gctgttgtcg gtttggagaa tttaaaatct
     6661 tttgccgacc cttgtactat ggagatgtca ggttccagct cagagtcttg gatctgtatt
     6721 cctttttcct gccggagaat ataaccggag ggaagtatgc cgttgaactc ttcaaacaga
     6781 atctgtgaaa aaaaatcata ttttgcgctt ttcgtcattt tctctatgat cattcctctg
     6841 aataattctg tctttttgta acgttccgga tctttagtac agatatcccg atagtcttgt
     6901 acactg
//
LOCUS       DAOQGJ010000007         6879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_5373_length_6879_cov_3.224502, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000007 DAOQGJ010000000
VERSION     DAOQGJ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5373_length_6879_cov_3.224502"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PK683_00235"
     CDS             complement(<1..1098)
                     /locus_tag="PK683_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06897.1"
                     /translation="MLFQTDAACNPSDPGNKNYYSCLHGGEIRKAEMNGLSDCTGITV
                     EDNANSTGGALNWKCTALNSKVIVYSTGFRTGDLGDMKNNPPPGLSDLIDWTVSPPKF
                     KLLKVTIKKNGILYSESESSSWWSNSFIHNTASASLNQSGTIYTFGSPSSDFDIGSGI
                     TGYYITAAKVAVTVSPGKKMFRGTAGGNTLVGSIAGSNFSWREGIFDAVNNSEGILLG
                     SNSYFHSLYNIRFQNLSGALRGINIQNANGFSRLKNISIGNTSGIGIQMSSGSGKNIF
                     ENISVFNNSLKGIEMSSSGSSSNLFINITSVSNLNSGILIDLNSPGNIIMNSTLSLNG
                     QTGMTVNSGSTFIMNSFISNNNSASEGFNCTV"
     gene            2075..2764
                     /locus_tag="PK683_00240"
     CDS             2075..2764
                     /locus_tag="PK683_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06898.1"
                     /translation="MKEFEKGFLLQKKEINRSFQKQEKDVKISELYSRYFKERTALDE
                     QLLNSYRSELERHIPKKGDLVLRKVNIEIDPRLTDPLLFKKELSLECTEKNLHYFPTA
                     KYSCFPSNPVTPPSEVDFSPVNREEWLNEWSETVFDERRLNHISEGNIVRCIFSYRNE
                     NHSENIQYAHYAQVLKKVSDSEYLIHISDLYSHKDDPGVNHLKIIHKSHIIEVPAEWN
                     PHLPEPEAWKD"
     gene            complement(2761..3207)
                     /locus_tag="PK683_00245"
     CDS             complement(2761..3207)
                     /locus_tag="PK683_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNH06899.1"
                     /translation="MENKKYYEDFSIEMKVDLGNYKVTKEEILEFASKYDPQPFHINE
                     KEAENSMFGGIIASGWHTASICMRLYVDKILNHSYSLGSPGVDEIRWKRPVRPGDILS
                     GKFIIIEKKPFKKDIGLIKGKAELYNQENRLVMTFIGNGMFKMKNA"
     gene            complement(3210..6452)
                     /locus_tag="PK683_00250"
     CDS             complement(3210..6452)
                     /locus_tag="PK683_00250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH06900.1"
                     /translation="MGFIDLFSAKVFFNFYSFGTLLVTIFTFLLALFFLTLPNKSKST
                     LHLGIGTFFLFLFNFGYFYASLYYSPDAAFHRWFTGGFILPAIIHLGQFFFHYPSDTR
                     PKLSKYALIIMWAVAIAVNGYFFYSTFPAPKKFHFTGHYWDFDAEPVSRFLATFIAIY
                     SFISFLLVMIWKAYITKGRDRRVIILMGISMLITALFPNITNIMSRDGAFERSTFLLT
                     LVFSFVLGFFLISIIYINSTTDKTSFMIKIVSITLVTMLIIMQLLSFFTMKDQDSDYD
                     GIHLDQAEKIIQNKDYYKDTEYVLELDMYTGKVEPVQYSKSHNLDLEIFKMDSQNTVL
                     YDTILNIPEGKFLKDIKIDLEGTPPHFEGYKLAILDFLKQFENKADEPVKAKTIAFIE
                     NLSSAGYINQNRISALDINNFCKEVTKYLNGKSELAFFKQAIYTNLKECSWNGMLLDN
                     VGLRREVTKYFRYFRPSQTRTYRRSMHSYEDQRHFVSYSIYNPYLNKVTEIGFSYTSY
                     RTHVHKMAANQQAILLLVVIAIITLYPLFFRGSLINPLDSLLYGVEKVNNGDLTVKVP
                     VKVRDEIGFLADSFNNMVTSIRTARIQLQDYAETLEEKVKLRTREVEEKMIEVQNLKN
                     QQDGDYYLTALLAQPLLRNANKSELVKTDFLIKQKKKFNFRNKIAELGGDICVTGNLR
                     FGKDKNNYKRYTFVMNGDAMGKSMQGAGGALVMGVMINSILARSARNDWVLNDSPDKW
                     LTDLYYDINRTFKSFNGSMVISCVVMLINDETGDVFYFNAEHPFSVLYKNGEARFIED
                     SMQLRKLGLDSEFPFRIFQTKLVPGDIVIIGSDGRDDIDLTPDEPMRTINEDEYLFLR
                     HVVKGQGDLHKIQESILGSGDITDDFSLLKITYKEQFKEEEADIIDLDEISQNKENDS
                     SSEEALDPEKVYFKSKDLLKEGENEEAFQLLFKLHSNYPNSPKITRLLGISAYRTKRY
                     IIAIEVMNNYLLKDPMFLEFWYYLGLSYKITGSYEKAEETAHHLMSMSPDHIQNIINL
                     ADVKRVLGKKEEAIHYTSVALNKDAGNPAAKKLMEMLRQGDTKDKLKALD"
     gene            6632..>6879
                     /locus_tag="PK683_00255"
     CDS             6632..>6879
                     /locus_tag="PK683_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06901.1"
                     /translation="MSQIRGLIDLVQDAISKGAASVEEVHRSIANQPLELLKQIQPLE
                     SSIQGFQDFQNQTIGGIYESIRSINQQAGEIARDLLSKV"
BASE COUNT         2033 a         1335 c         1350 g         2161 t
ORIGIN      
        1 gaccgtacag ttgaatcctt ctgatgctga attgttattt gaaatgaatg aattcataat
       61 gaaggtgctc cctgaattca cagtcattcc tgtctgaccg ttcagactca gtgtggaatt
      121 cataatgata ttgccgggag agttcaaatc aatgagaatt cctgagttaa gattggaaac
      181 tgaagtgata ttgatgaaaa gatttgaaga tgatccggaa ctgctcattt caattccttt
      241 cagggaattg ttgaagacag aaatgttttc gaatatgttt ttacctgatc cgctggacat
      301 ctggatgcct attccggatg tatttccgat ggaaatattt ttcagtcttg agaaaccgtt
      361 tgcattttga atgttgatac ctctcaatgc gccgcttaaa ttttgaaatc ttatattgta
      421 aagtgagtga aaataagaat tggaacctaa tagaatccct tcactgttgt tcactgcatc
      481 aaaaattcct tctctccaac tgaagttgga accggcgatg ctccccacaa gtgtgttccc
      541 gcctgcagtt cctctgaaca tcttttttcc agggcttaca gtgactgcca ctttagctgc
      601 agtaatataa tagcctgtaa tacctgaacc aatgtcaaag tcggaagaag gagatccaaa
      661 ggtatagatt gttcctgact gattcaatga cgcggatgct gtgttgtgaa tgaaactgtt
      721 cgaccaccag gaggaacttt cagattcaga ataaaggatt ccgtttttct ttattgtaac
      781 cttcagcagt ttgaatttcg gcggagatac tgtccagtca ataagatctg acagtccggg
      841 tggagggtta tttttcatat cacctaaatc gccggttcta aatcctgtcg agtagacaat
      901 tactttactg ttgagtgcag tgcatttcca attaagtgca ccgcctgtgc tgtttgcatt
      961 gtcttccact gtgattcctg tacagtctga cagcccattc atctctgcct ttctgatttc
     1021 tcccccgtgc aggcaggaat agtagttttt gtttccaggg tctgagggat tgcaggctgc
     1081 atctgtctga aatagcatac ctttagaata gtctctctga atatagtcat tccaattttt
     1141 tccatttacg gaaaagaagg gacttaggct gaaagcagtg cacgtaatat ctgcgttttc
     1201 gagagatgtc ttaaatacgc ctgacgagtt tattattgtg cattgaaaag aggatggatg
     1261 cgttttaatt tcaatgctgt aggaatcacc gactttgatt cctgtaaatt tgtaactgac
     1321 tgcacctgag tttaaggata tttctgatgc actatttttt aataccagcg ttcctgaatt
     1381 gaacccggct atttttcctc caatgctccc cccgctatca acttgagaaa aaaaggcaaa
     1441 aaggaatcct gacggagatc gaaaatccca tcctgatttt tttgtttgaa ggcagaaaga
     1501 tatggtgaga cataaaaaaa tgatagagaa ataaaacctt ttcattgata tcctccaaaa
     1561 cctcaactat caccctccct ttagaatgac atttgagcca aggtttttta tcaaagcagt
     1621 cattttttca ttgactctgt tttttataat gaaaaatata tataacaaga ttatacaagg
     1681 atttgaaatg cctgagttgg aatcgtttaa tgccttatct gatgttgatg gaaggaagct
     1741 taaagtaaat cccaaagagg gtgatatcgt cagtgctgtc ctgaggaaag agcttccata
     1801 ttcacaattg atgcattttg accaaagatc tgatgaaaac agacatattc aggattctag
     1861 ctttccagtt tcataccgag aagaggtctt ttttaaagtt tcaaaaatgc agaatgggaa
     1921 aattcacctg gaatttcata atttttttgg aaatgataag gttttaagga gacatattac
     1981 ttatactgga catcaccaaa cagtcattga tgagaattcg agggaaagct tattcaggct
     2041 ggaatacaca aaaagatgtg ctgaattcaa agaaatgaag gaatttgaaa aaggattcct
     2101 tttgcagaaa aaagaaataa acaggagctt tcaaaagcag gaaaaggatg taaaaatttc
     2161 tgagctgtac tcccgttact ttaaagaaag aacagcttta gatgaacagc tgctcaattc
     2221 ttacagatca gaattggaaa ggcatattcc taaaaaaggt gatcttgtac ttcgaaaggt
     2281 caatatagaa attgatccaa ggctgactga tcctctgctt tttaagaaag aactttcatt
     2341 ggaatgtact gaaaaaaatc ttcattattt ccctactgca aagtattcct gtttcccgtc
     2401 aaaccctgtt acgcctccgt cagaagttga tttttcacct gtgaaccgcg aagagtggct
     2461 gaatgaatgg tcagagactg tttttgatga aaggagacta aatcatattt ccgaaggaaa
     2521 tatagtcaga tgcatttttt cctatcggaa tgaaaatcat tcagaaaata ttcaatatgc
     2581 tcattatgcg caagttttaa aaaaggtctc tgattcagag tatctgattc atatttcaga
     2641 tctttactcg cataaggatg atcctggagt aaatcatttg aaaataatcc acaaaagtca
     2701 tattatcgaa gtgcctgcag aatggaaccc tcatctgcct gaaccggaag cctggaaaga
     2761 ctaagcgttt ttcattttga acattccgtt gccgatgaat gtcattacca gtctgttttc
     2821 ctgattgtaa agttctgctt ttcctttgat aagacctatg tctttcttga agggtttctt
     2881 ttctattata atgaattttc cagataaaat atcccctggc cggacaggtc ttttccagcg
     2941 gatttcatca actcccggtg aacctaaact gtatgaatga ttcagtatct tgtctacata
     3001 cagcctcata cagatgcttg ctgtatgcca gccgcttgca atgatcccgc caaacattga
     3061 gttttcagct tctttttcat tgatatgaaa cggctgagga tcatatttag aagcaaattc
     3121 cagaatttct tccttggtga ctttataatt ccctaaatcc actttcattt caatggaaaa
     3181 atcttcgtaa tactttttat tttccattat caatccagtg ccttcagttt atccttggtg
     3241 tctccctggc ggagcatttc catcagtttt ttggcagctg gatttccagc atccttgttc
     3301 agcgcaacag atgtataatg aatcgcttct tcttttttcc caagcacacg tttgacatct
     3361 gccagattaa tgatattctg tatatgatcc gggctcatac tcatcaaatg gtgagctgtt
     3421 tcttctgctt tctcataact tcctgtaatt ttataggaca ggcccaggta gtaccagaat
     3481 tcaagaaaca tagggtcttt caaaagataa ttattcatta cttcgatcgc aatgatgtat
     3541 ctttttgttc tgtatgccga tattcccaaa agtcttgtaa tcttaggact gttgggataa
     3601 tttgagtgaa gcttgaaaag aagctgaaag gcttcctcat tttccccttc cttcagaagg
     3661 tctttcgatt tgaaataaac tttttcggga tccagtgctt cttctgagga tgaatcgttt
     3721 tctttgttct gtgagatttc atccagatca ataatatcgg cttcttcttc cttgaactgt
     3781 tctttatagg taatcttcag aagagaaaag tcatctgtaa tatcacctga accaagaatg
     3841 gactcctgaa ttttatgcaa gtctccctgt cctttgacta catggcgcag aaacagatac
     3901 tcatcctcat tgatggttct catcggttcg tccggtgtca ggtctatatc atctcgcccg
     3961 tctgatccga taataactat atcgcctgga accagttttg tctgaaaaat tctgaacggg
     4021 aattctgaat caagtccaag ttttctcagc tgcattgaat cctcgatgaa tcttgcctcc
     4081 ccgtttttat aaagaacaga gaaagggtgc tctgcattaa agtaaaacac atcacctgtt
     4141 tcatcattaa tcagcattac tacgcaggaa ataaccatac tgccgttaaa cgacttgaac
     4201 gttctgttga tgtcataata aagatcagtc agccatttat caggagagtc gttcaagacc
     4261 cagtcatttc tggcagaacg cgcaagaatt gagtttatca taactcccat aaccagcgca
     4321 ccaccggctc cctgcattga tttgcccatt gcgtcgccgt tcatgacgaa tgtatatctt
     4381 ttataattat ttttatcttt tccgaaacgg agatttcccg taacacagat atcccctccg
     4441 agctctgcta tcttgttcct gaagttgaat ttttttttct gctttatcag aaagtctgtt
     4501 ttgacaagtt ccgatttgtt tgcatttctc agcagcggct gagctaaaag tgcagtcaga
     4561 taatagtcac catcctgctg atttttaagg ttctgcacct caatcatctt ttcttccact
     4621 tcccgcgttc tcagtttcac tttttcttcc agagtttctg cataatcctg caactgaatt
     4681 ctcgcggttc gaatggaagt caccatattg ttgaatgaat cagccagaaa accaatttca
     4741 tctctgacct tcacgggaac cttcactgtc agatctccgt tatttacttt ctcaaccccg
     4801 taaagaaggg aatcaagcgg gttaatgaga cttcctctga agaacaaagg atataatgta
     4861 atgattgcaa tgactacaag aagaagaatt gcctgctgat tggctgccat tttatgaacg
     4921 tgggttctgt agctggtata ggaaaaacct atctctgtca ctttgttcag ataagggttg
     4981 tatatgctgt aagatacaaa atgacgctga tcctcataac tgtgcatact tcttctgtat
     5041 gttcttgtct gtgatggacg gaaataccgg aaatactttg ttacttctct tctcagtccg
     5101 acattgtcca aaagcattcc gttccaggag cattctttca gattagtata tatagcctgt
     5161 ttaaagaagg ccagttctga ttttccattc agatattttg tgacttcctt gcaaaagtta
     5221 tttatgtcaa gtgcactgat cctgttctga ttgatataac ctgctgaact taagttttca
     5281 atgaaagcga tagtttttgc ctttacaggc tcatccgcct tattttcaaa ctgcttcagg
     5341 aaatccagaa ttgccagttt gtagccttca aaatgaggag gagttccttc cagatcaatc
     5401 ttaatgtctt tcaggaattt cccttcaggg atattgagta ttgtatcgta aaggacagtg
     5461 ttctgagaat ccatcttgaa aatttccaaa tcaaggttgt gtgatttgga atattgaaca
     5521 ggttccactt ttccggtgta catatccagt tccagtacat actctgtatc tttatagtaa
     5581 tctttgttct gtatgatttt ttctgcctga tccagatgga ttccgtcata gtcagaatcc
     5641 tgatctttca tcgtaaagaa gctgaggagc tgcataataa tgagcattgt aacaagcgta
     5701 atgctcacga tcttgatcat aaaactggtt ttgtccgttg tggaatttat ataaataatg
     5761 gaaattagaa agaacccaag aacgaaagaa aacacgagcg tcagaaggaa agtggatctt
     5821 tcaaatgcgc cgtctcggct cattatattt gtgatgtttg gaaagagggc tgtgatgagc
     5881 attgagatcc ccatcagaat aatgacgcgc ctgtccctgc ctttggttat gtacgctttc
     5941 cagatcatta caagcaggaa tgagatgaaa ctgtaaatag caatgaatgt cgccagaaat
     6001 ctgctgacag gttctgcgtc aaaatcccag tagtggccgg taaaatgaaa ttttttagga
     6061 gcgggaaagg tggaatagaa aaagtaacca tttacagcaa ttgccactgc ccacattatg
     6121 atgagggcat actttgaaag tttcggtctg gtgtctgaag gataatggaa gaagaactgt
     6181 ccaagatgaa tgattgccgg caggataaat cctccggtaa accatctgtg aaaagcggca
     6241 tcaggtgaat agtacagaga ggcataaaag tatccgaaat tgaatagaaa caggaaaaat
     6301 gttcctatgc ccagatggag agtacttttg gatttgttcg gcagtgtcag gaaaaaaagt
     6361 gccagaagga aagtgaaaat agttaccagc aatgtcccga aagaatagaa gttgaaaaac
     6421 acttttgctg aaaaaagatc aataaatccc atactgaata agcctcgagg gaatagtata
     6481 taagattagc aattaaatga aatcattttt tttatttttt cggtgaaagc ataattagat
     6541 ctgtaaaata cttgcggaaa aaacgaattc atacaaagta agcatcctgt ccgcaatttt
     6601 tcgacaggaa gaataaatat taggagagta tatgagccag ataagaggac ttattgattt
     6661 agtacaagat gcaatcagca agggagcagc aagcgtagag gaagttcaca gatccattgc
     6721 aaatcagcct ttggagcttc tcaagcagat tcagccgctg gaatcttcca tccagggatt
     6781 tcaggatttt cagaatcaga caataggcgg aatctatgaa tccattcgca gcatcaacca
     6841 gcaggccggt gaaattgcca gagatctgct ttccaaagt
//
LOCUS       DAOQGJ010000008         6720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_5573_length_6720_cov_3.066017, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000008 DAOQGJ010000000
VERSION     DAOQGJ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5573_length_6720_cov_3.066017"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PK683_00260"
     CDS             <1..326
                     /locus_tag="PK683_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH06902.1"
                     /translation="GDDARRKEDIPDDKFVQSAMKVLRKLFDKDAPDPDEILRTNWGK
                     DEFSYGSYSHIPPGAESEDYDAIAEPVNDVLFFAGEGTSSEYPGTIHGALISGRRAAE
                     EILDL"
     gene            complement(327..1502)
                     /locus_tag="PK683_00265"
     CDS             complement(327..1502)
                     /locus_tag="PK683_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002743660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH06903.1"
                     /translation="MDFTVPKEVEELRLKVRKHIEEKIIPLEDHYSYDTGRLPEEISQ
                     KAREEVKKAGLWTPHLPKSEGGLGLNHVGTAIIFSELGRSPIAPYIFNCDAPDEGNMH
                     LLHVAANEKQKEKYYFPLIQGKVRSGFAMTEPPPGTGSDPSSLLTDAVKSGDKYIING
                     RKWFCTGANGADYLIVMAKVNGSFRRTTMFFVPTNAKGYTMVREVVTMGSHGPGGHCE
                     LNFENVEVPEENILGKVGEGFRLSQVRLGPARLTHCMRWLGLARRSMDIAMEYAKKRE
                     VFGSKIAEHQGIQWMFAESALEIESGYMLTLKAADMLDKDMDTRQAISMAKWSVSETL
                     CRVIDRAIQICGATGYSRDMKLELFYRDARAARLADGPSEVHKMVIGRNLVSGKASF"
     gene            complement(1766..2095)
                     /locus_tag="PK683_00270"
     CDS             complement(1766..2095)
                     /locus_tag="PK683_00270"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH06904.1"
                     /translation="MSRRKIHSESGPDLSIVLGSLDDFRDSRPATAEWVIEVAVSTLS
                     LDLSKRKIYAEANIPHYWIIEPENGRILLFSNPTSEGYISESVLRTTEEIHLPIEPFS
                     SIPMDWI"
     gene            2157..2594
                     /locus_tag="PK683_00275"
     CDS             2157..2594
                     /locus_tag="PK683_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06905.1"
                     /translation="MNITECRLQTEGDLSFRIQKAYFESGAEKRSKAVLIIKFAGFHG
                     EGSGGNRNSEFMKTVISASLGLFLSDMLILDLTELKYEFGDSLASVLDIPSGIKSRDY
                     ETVLAVSDLCRKGLLSLMEFTQVSFRTFSGLEEALNYFSGLKI"
     gene            2697..4871
                     /locus_tag="PK683_00280"
     CDS             2697..4871
                     /locus_tag="PK683_00280"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH06906.1"
                     /translation="MQCISLTMKVTLSIRNGFILSFILLISCILLIFFIGYRVIFSDV
                     AKDLNKGLESMSFLAADLVDRSLYRVANSTKALADAYRENYRKTKFTEKKSRLDSSVK
                     RTSKTASFELCTENDPKFKSPCLSYLLYNDFQFDPVIYRELQTAEKLEPVFKNMYDTF
                     AYSWVYLTTVNDFLFIYPYIPYKDADGIYKPTRQHFYLAADFHNRTFGWEEPYLDLAG
                     EGMMITVSYPIYDGDDLLGVASRDITLHQLSSSVLEKTMAYSGTTSFIVDKNGKAISS
                     SSEEYLNEMDEVNKKDYRGILYYRQKEGLEKIGKKGITGSKFSLMNDITELVLYEFQN
                     TGKKIINLDFTMKEKYKVIASRSSAAGWFIISVIPDRSVYEASQYSFLRLFSFVLIAV
                     FSIYAIAGYWAVKMVVRPLGILSRSAERVSKGDLSVRMPALYTDEIGQFSRIFNQMLK
                     NMEKAKYELEDYSRNLEQKVEERTDALNSSLQLIKKDMNFAKQIQARLLPEYENLHER
                     LRIACRYAPMDEVGGDIYDIIKLKDGTVRIYIADATGHGVQAALVTMLIKSSFESLKE
                     TERHPSDILFSLNNLFCSNYKSLNSYFSCFVVDINPKKQTLVYSSAGHPAQLLLKSNG
                     ETEHLNKTGRIIGMKGKAVYKNEKIGFRPGDRLLLFTDGVFEEFNESEEEFGEERLLE
                     SAKASSMKPVSEMLESINLALEEFLKSSPKQDDITVIGIEYI"
     gene            complement(5039..6121)
                     /locus_tag="PK683_00285"
     CDS             complement(5039..6121)
                     /locus_tag="PK683_00285"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNH06907.1"
                     /translation="MKSILKDYIEEHKITGMAACIIKDGKIIWKECAGYADVSKKKPV
                     TADTSFVLASIGKTFTAVAAMTLVEKGKLNLKDDVNEYLPFEVRNPNYPDEPITVEML
                     MTHTSSIIDDRQYNMDDALVYFRNKDPKITLAEYVEKYITPDGEYYVEDECFSKEKPG
                     KKYSYSNAGVALLGLIIERVSEMGCDEFCRKKIWSPLGMKNTAWRLADLDMENASLPF
                     YEYHKTKGHYNCPDYPNGLYKSSLSDLAKFLMMFMQDGTYDGETIISEKTAKLMKKEH
                     FSDDENSVGLIWCLSDDLIGHDGAEEGSSTTMWYDTESNIGVIILCNQSDVDLEDLYE
                     EIYEMADEDSEGSEDEDEEDSEEDED"
     gene            6304..>6720
                     /locus_tag="PK683_00290"
     CDS             6304..>6720
                     /locus_tag="PK683_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06908.1"
                     /translation="MLFFHCRVFCVKLQLGAFLLVLCSVFCTKSAEHPKAVQGRLDLR
                     HLNENFFEKDSLPLNGEWEFYRNTHIPASDNETVSVHTYEKVPNNWTSYIKDGREFPS
                     LGIGSFRLKILLKEKCTEMGIRIFTFGTSYSMYVDGN"
BASE COUNT         1918 a         1499 c         1378 g         1925 t
ORIGIN      
        1 caggcgacga tgcacgcaga aaagaagaca tccctgacga taaattcgtg cagtctgcaa
       61 tgaaagtcct tcgaaaactt tttgacaagg atgctcctga tccggatgaa attctccgaa
      121 caaactgggg aaaggatgaa ttcagttacg gttcctattc tcatatcccg ccaggagcgg
      181 agtctgagga ttatgatgca attgcagaac cggtaaatga tgttctgttc tttgcagggg
      241 aaggcacaag ctcagagtac cccggcacaa tacacggggc actcatttca ggaaggcggg
      301 ctgcagaaga aattctggat ctttgatcag aagcttgcct ttccgcttac caagtttctg
      361 ccgatgacca tcttgtgcac ttcagatggt ccgtctgcaa gtcttgcagc tctggcatcc
      421 ctgtaaaaca gttccagttt catatctcgg gaatatcctg tcgcaccgca gatctgaatt
      481 gccctgtcga tgaccctgca gagagtttca ctcacagacc attttgccat ggagatggcc
      541 tgtcttgtat ccatatcctt gtcgagcata tccgctgctt tcagagtcag catataaccg
      601 gactctatct ccagcgcaga ctcggcaaac atccactgaa ttccctgatg ctctgctatt
      661 tttgaaccga agacttctct tttttttgca tactccattg caatgtccat ggagcgtctg
      721 gcgagaccaa gccatctcat acaatgagtg agtcttgctg gtccgagtct tacctggctc
      781 agcctgaatc cctctcctac tttccctaag atgttttctt caggcacttc cacattttca
      841 aaattcagtt cgcagtgacc acccgggcca tgagaaccca ttgtcaccac ttcgcgcacc
      901 atagtgtatc cttttgcatt tgtaggcaca aagaacatcg ttgtccttct gaagcttccg
      961 tttactttcg ccattacgat cagatagtct gcaccgtttg cacctgtgca gaaccatttt
     1021 cttccgttga tgatgtattt gtcgccggac ttgactgcat ctgtaagaag agaggaagga
     1081 tcagaccctg tacccggagg aggttcagtc attgcaaatc cggaacggac ttttccctga
     1141 ataaggggga aataatattt ttccttctgc ttttcatttg ccgcaacatg aagaagatgc
     1201 atattccctt catcaggcgc atcacagttg aatatatagg gagcaatcgg agaacggcca
     1261 agttcgctga atataattgc agtgcccaca tggttcagtc ccagtcctcc ttctgatttt
     1321 ggaagatgag gagtccagag tcctgccttt ttcacttctt ctcttgcctt ctgtgagatt
     1381 tcttccggca gtcttcctgt atcataactg taatggtctt caagaggaat gatcttttcc
     1441 tctatatgct ttctgacttt cagtctcagt tcttccactt ccttaggcac tgtaaaatcc
     1501 ataatatcct ctcctgtttc agctgttcca ccggcaggct tcacagtcaa cataaacctg
     1561 atataacaac ttgcttttaa aaagcaagaa aaggttgtcc ctttcgggag aatcgtactt
     1621 gtctgatttc agaccctgct tttaagtttc tttccttgaa ctgatcttat tcggtatgct
     1681 catcaggcag ccgctctcag ccgacaggcc gacaaatcga aaattgagtc cctgtcctct
     1741 ctggagcatt ataatttatt ctgtattaaa tccagtccat aggaatggaa gaaaagggtt
     1801 ctatcggcag atgaatctct tccgtcgttc tgagaacaga ctctgaaata taaccctctg
     1861 aagtcggatt ggaaaaaaga aggattcggc cattctccgg ttcaatgatc caataatgag
     1921 ggatattcgc ttctgcataa atttttcttt tgctcagatc aagactcaga gtggaaacgg
     1981 ccacttcgat cacccattct gcagttgcag gacgagaatc acgaaaatca tccagactcc
     2041 ctagaacaat gctcagatcc ggtccagact ctgaatgaat cttccgtctg gacaaaaata
     2101 acttgctgat tttcatctgt atataatctg tctttctgat attaagtgag gacctattga
     2161 atattacgga atgcagactt cagacagagg gtgatctttc cttccgtatc caaaaagcgt
     2221 actttgaatc cggtgcagaa aaacgcagca aggcagttct gatcattaag tttgccggtt
     2281 ttcatggtga gggaagcggg ggaaaccgaa attctgaatt tatgaaaaca gtgatttctg
     2341 catcccttgg actgttcctc tcggatatgc tgatcctgga tctgacagaa cttaaatacg
     2401 agtttggaga ctcccttgca tcagttcttg acattccttc aggaataaaa tccagggact
     2461 acgaaactgt tctggctgtt tccgatctct gcagaaaggg gctgctttca ctgatggagt
     2521 tcactcaggt ttcattcaga acattcagcg gactggagga agcactcaat tacttttccg
     2581 gattgaaaat atgattgcag taattcataa aaaaaattaa tttactattt tgcatcaact
     2641 gcatttagaa aaaacagacg tccgcagcag agggattccc gaagtttgcc ccgtctatgc
     2701 agtgcatatc tctgacaatg aaagtgactt taagcatacg aaacggtttt attctttcct
     2761 tcatcctgct gatttcctgc atcctcctca tattttttat tggatacaga gtcatttttt
     2821 cagatgtggc aaaggatctg aacaagggtc tggaatcgat gagtttcctt gctgcggatc
     2881 ttgtggacag atcactgtac agagttgcca acagcacaaa agcacttgcg gatgcctaca
     2941 gggaaaatta cagaaaaaca aaattcacag agaaaaaaag cagactggac tcttctgtca
     3001 aaagaacatc aaagacagcc tcattcgaac tctgcactga aaacgatcca aaattcaaat
     3061 ccccctgtct gagttatctt ctttacaatg atttccagtt tgatccggtc atttacagag
     3121 aacttcagac agctgaaaaa cttgaacctg tgttcaagaa tatgtacgat acattcgctt
     3181 attcatgggt gtacctgact actgtaaatg atttcctttt catttatcct tacatcccct
     3241 acaaggatgc ggacggtatc tacaaaccga caaggcagca tttttacctg gcagcagact
     3301 ttcacaacag aactttcgga tgggaagaac cttatctgga tctggccggt gaaggaatga
     3361 tgattacagt ttcgtatccc atttatgacg gggatgatct gctcggtgtc gcatccaggg
     3421 atattaccct tcaccagctt tccagttccg tattggaaaa gacgatggcc tattcaggaa
     3481 caacttcgtt cattgtggat aaaaacggaa aggcgatttc ctcttccagc gaagaatacc
     3541 tgaatgagat ggatgaggtg aacaaaaaag actacagagg aatattgtac tacagacaga
     3601 aggaaggact tgaaaagata ggaaagaaag gcattacagg ctcaaaattc agcctgatga
     3661 atgacatcac tgaactggtg ctttacgaat ttcagaatac aggaaaaaaa attataaatc
     3721 ttgattttac aatgaaagaa aaatacaaag taatcgcatc cagatcctct gctgcaggct
     3781 ggttcattat ttccgtcatc cctgacagat cagtctatga agcttcccag tattcttttc
     3841 tcagactttt cagctttgta cttattgcag tcttctcgat ttacgcgatt gctggatact
     3901 gggctgtgaa aatggtagtc agacctttgg gaattctttc cagatcggca gaaagggtca
     3961 gcaagggaga tctttccgtc agaatgccgg ctctttatac agatgaaata ggacagttct
     4021 ccagaatatt caaccagatg ctgaagaata tggagaaggc aaagtatgaa cttgaagact
     4081 acagcagaaa tctggaacag aaagtcgagg aaagaaccga tgcactgaac agttcactgc
     4141 agctgatcaa gaaggatatg aactttgcga aacagattca ggcaagactt cttccggaat
     4201 atgaaaacct tcacgagcgt ctgagaatcg cctgcagata tgcgccaatg gatgaggtag
     4261 gcggagatat atacgatatc attaaactga aagacggaac agtaagaata tatatcgcag
     4321 atgcaaccgg tcacggagtg caggccgcac ttgtcacaat gctgatcaaa agcagctttg
     4381 aatctctgaa ggaaacagaa aggcatcctt cggatattct tttcagcctg aacaatctgt
     4441 tctgctcaaa ctacaaatcg ctgaattcct atttctcctg tttcgttgta gacatcaatc
     4501 cgaagaagca gaccctagta tattcttctg ccggtcatcc tgcacagctt cttctgaaat
     4561 ccaacggtga gacagaacat ctgaacaaga ctggaagaat cattggaatg aagggaaaag
     4621 cagtctataa aaatgaaaaa atcgggttcc gcccgggaga cagacttctt cttttcactg
     4681 acggagtatt cgaggaattc aatgaatcag aggaggagtt tggggaagaa aggcttctgg
     4741 aatctgcaaa agcatcttca atgaaaccgg tttcagagat gcttgaatcg atcaaccttg
     4801 cactggaaga atttctaaaa tcctcaccta aacaggatga cattacagtc attggaatag
     4861 aatatatctg atttgcggca ggggaacctg cctgccgctg tactatgcaa atatggaaac
     4921 ggatgcaagc aaaccaagga gacagtaaga tcaaggatta cttgcaaaat agtgcaataa
     4981 attccggtga tccgcataaa acaaatttta gtcatcgtct cccaatttat tgcaggaatc
     5041 agtcctcatc ctcctcactg tcttcctcat cttcatcttc agatccttct gaatcctcat
     5101 cagccatttc atagatctcc tcataaagat cttccagatc cacatcagac tgattgcaga
     5161 gaataatgac tcctatattc gactcggtat cgtaccacat tgtagtgctt gatccctctt
     5221 ctgccccgtc atgtcctatc agatcatcac tcagacacca gatcagtcct actgagtttt
     5281 catcatcgga aaaatgctct tttttcatca gcttagctgt tttttcgctg ataattgttt
     5341 ccccgtcata ggtgccgtcc tgcataaaca tcatcagaaa ctttgcaaga tcagaaagac
     5401 ttgacttgta taagccgttt ggatagtcag gacaattata atgtccttta gttttatggt
     5461 actcataaaa aggaagagag gcattctcca tatccagatc ggccagtctc caggcagtgt
     5521 ttttcattcc gagaggcgac cagattttct ttctgcagaa ctcgtcacag cccatttcgg
     5581 aaactctttc tatgatcaga cccagaagtg caactcctgc atttgaatag gaatattttt
     5641 tcccaggttt ttcctttgaa aaacattcat cttccacata gtattccccg tccggtgtaa
     5701 tatatttttc tacatattct gccaaagtga ttttaggatc cttgttcctg aagtaaacaa
     5761 gtgcatcatc catattgtac tgcctgtcgt cgatgatgct ggatgtatga gtcatcagca
     5821 tttccactgt gatcggctca tcaggataat ttggattgcg cacttcaaaa ggaagatact
     5881 cattgacatc atctttgaga tttaatttgc ctttttcaac aagagtcatt gccgcaactg
     5941 ccgtaaatgt cttgccgatt gaggcaagaa caaaagaagt atctgcagta acaggtttct
     6001 tttttgagac atcagcataa cctgcacact ctttccagat gattttcccg tctttaatga
     6061 tacaggcagc catcccggta attttatgtt cttcaatata gtctttaaga atggatttca
     6121 ttccgaacac ttgaccagat tcttttcttt ttgcaaatac atttttcaat tcttgacatt
     6181 ctgcagacgg tttctccagc agaactacag gctggctcta tttgagaaaa acttggacag
     6241 attttacttg agctggatga attaaaactc atactgtcag gatatgccga cagaagagaa
     6301 cagatgcttt tttttcactg cagggtattc tgcgtgaaac ttcagctggg agcatttctt
     6361 ctggtacttt gctcagtatt ctgcacaaaa tcggcagaac atccgaaagc agttcaaggc
     6421 aggcttgatc tcagacatct taacgaaaat ttttttgaga aagacagcct tcctttaaac
     6481 ggagaatggg aattttatcg gaacacacat attccggcat cagacaatga gacggtatca
     6541 gtccatacat atgagaaagt gcctaacaac tggacatctt acataaagga cggcagagag
     6601 ttcccttctt tggggattgg ttcatttcgc ctgaaaattc tgctgaagga aaaatgcact
     6661 gaaatgggga tcaggatttt tacgttcgga acgtcctatt cgatgtatgt cgacggaaat
//
LOCUS       DAOQGJ010000009         6697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_5608_length_6697_cov_3.374737, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000009 DAOQGJ010000000
VERSION     DAOQGJ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5608_length_6697_cov_3.374737"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK683_00295"
     CDS             complement(<1..249)
                     /locus_tag="PK683_00295"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH06909.1"
                     /translation="MKSGFFLNSPRRGFAEPARLAILQVLRKGAVTVSDIVQATSLSQ
                     SNVSNHLNCLKDCGLVNSEQQGRFVYYQLSDERIAKLLD"
     gene            complement(280..882)
                     /locus_tag="PK683_00300"
     CDS             complement(280..882)
                     /locus_tag="PK683_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06910.1"
                     /translation="MDTEKNSAKKQEQWSDELGEMDWDSAKAKCESLRMRLPAVSEIQ
                     SVHKSEIASSWEQSWHWTYEETVSGKAYAVDVENGRAELNRKGNHCIARCIKTEKKPA
                     ERYSENSTLGKWSERMGVMNWDSAFKKCESSGMRLPSRTEIETAFNSGRIFFSEFEGG
                     NWTAEHSYRTEEPYIFYVRDGNILLGSRKGSSSYGVRCIR"
     gene            1252..2187
                     /locus_tag="PK683_00305"
     CDS             1252..2187
                     /locus_tag="PK683_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNH06911.1"
                     /translation="MKSKIISLMIISSLIYCSSEQIREKPISPFLDTQGHRGARGLKP
                     ENTIPAFETAMQYGMRTIELDTVVTKDKKLIVHHDTETNPVICRNSDGTPIVSRKISE
                     LTVSELKDLDCGSPVNPKFPEQKPVPGTKLSTLDEFFQSVKDYEKKNKIKTPFLFNVE
                     TKFDKNPTEEETKEFAALMTDYIRKAGVTDRTTVQSFHLPVLPEVRKIEPKLKLSALV
                     EPGYFGGLRMYLGLGAGVRKSAILKAEELKVDVLSPYHLYVTPEFVREAHKRNIQVIP
                     WTVNETDLMKSLLEKGVDGIISDYPDRLSAAVEKK"
     gene            2235..3758
                     /locus_tag="PK683_00310"
     CDS             2235..3758
                     /locus_tag="PK683_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06912.1"
                     /translation="MRELFKWSSGNRFFGALYLSALIHFVLLVLVLSPISEYDPVFCG
                     YHILKLVPKEKSALEFEFGSSEDGNSSLEKGSQQQPSPPTGNPSREEGKSLAENQGKI
                     KEKYEGTEWKDLVENLEKTKDLRKNFKEDFQNNILQNGGATDRYIKRYRHYEDMVVKE
                     VLPTLGSIDEPFSKNLDKADEVLEKHRERNEIIEKFRSGDGDEPTKVEIAKKSPNRSE
                     TPLKMSEEEMNQYLDETLKNHKEEQMEEFRQKYANGYDPDKGDLPKLFRELYYKNLRR
                     IAYNFSPDPTYFAADYFEENLNKEDYLRNAMELVKKWKGTKTATEILFTIFDIYSIQE
                     RAVGEVLQMKTVLKNQNSADKNQIRSETIRRITEKYDPVLTKKGIKNSADASKLYDAK
                     KLEILNYILETSPEGYRKNDALFEKGRVLFEKSYRSNSQSFEEAAEVWRKIQPEKDKE
                     FIFSGSYERLKSALKEIEKNPSSAGIMRMQVLNILQMREAESIEKKREREQRLLWGN"
     gene            3766..4572
                     /locus_tag="PK683_00315"
     CDS             3766..4572
                     /locus_tag="PK683_00315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH06913.1"
                     /translation="MKKVILITGSTDGIGKNTARILASQGHKIILHGRSEDKCLSARK
                     ELLSENPKAEVDFASGDLSSMKQTARLADTVMKKTDRLDVLLNNAGVFMNEKKLSEDG
                     FELSFAVNHLAHFLLTMRLLDFLKKNSGFRIVNLSSVAHRNGVFDPENLQAEKNFTGY
                     GAYALSKLANILFTMELSEKIKGKGTSNCMHPGVIGTKLLKTGFNMSGGTLDEGSDNS
                     VYLSVSPEVQEITGKYFVKREVSPVNPKLIPKEHRKQLWEISEKMTSEFL"
     gene            complement(4621..5451)
                     /locus_tag="PK683_00320"
     CDS             complement(4621..5451)
                     /locus_tag="PK683_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06914.1"
                     /translation="MKKKKTAVKKKTIAKKKTASKKKKNTKAAQKRKMIAEAEARALQ
                     EQLESENAKKEELEALRKVLSKAVTRKSEFNLDVLDNFELRILMSSLAGFIYETHALY
                     SFEYDGKEYHNQPNQWYFSEYEGFPHDPVTRCTGDDECEFEEGEEFVRVKPKKGKKIK
                     YNIDWVGKNFDERSNPLLFEIGKVFGKNSILHSRPLILINESFSEDHRGSDHTRIIMD
                     FPGKVEFKTKKTDMDDFIDALFRLRSHKFENWYEMYLRSEVNSDKKVIIMDVIFDHGS
                     "
     gene            complement(5586..6398)
                     /locus_tag="PK683_00325"
     CDS             complement(5586..6398)
                     /locus_tag="PK683_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06915.1"
                     /translation="MKKKKTAVKKKTASKKKKNNKAAEERKMIAEAEAKALKEQQEWE
                     NARKEEQAVLRKVLSKAVTRKSEFNLDVLDDSELKILLSALIRFIYDTHTFFSFEYDG
                     KEYHNQPNQWYFGEFDGFPHDPVTPCKGYDECEFEEGQKLVRVQPKPGKKIKYNIDWE
                     GREFDEESKPLVYEIGQVFSQNPILHSRPLILINENFNDDHRGSDHTRIILDFPGKVA
                     LKGKKTKMTDFVDALFRLRSHKFENWYEMYYGAEVSSDKKAITVDVMFDHGS"
BASE COUNT         2028 a         1453 c         1304 g         1912 t
ORIGIN      
        1 atccagcaat ttagcaattc tttcatcact tagttggtag taaacaaatc ttccctgttg
       61 ttcagaatta actaatccgc agtcttttag gcaatttaag tgattggaaa catttgattg
      121 gctaagactt gttgcctgca caatatcact aactgtaact gctcctttgc gtaaaacctg
      181 caagatagct agccttgcag gctctgcaaa acctcgccgg ggcgagttga ggaaaaatcc
      241 tgattttata atgaaagagt caactcaccc agaccacaat cagcggatac agcggacacc
      301 gtaactgctg cttcctttgc gggagccaag gagaatattg ccgtctcgga cataaaaaat
      361 atatggttct tcagttcgat aggaatgctc agctgtccag tttcctccct caaattctga
      421 aaaaaagatc cgtcctgaat taaatgcagt ttctatttca gttctggatg gaagcctcat
      481 tcctgaactt tcacattttt taaatgcgga atcccagttc ataactccca tacgttcgct
      541 ccattttccc aaagtcgaat tctctgaata cctttcagca ggtttttttt cagttttaat
      601 gcaccttgca atacagtgat tgcccttacg gttcaattct gctcttccgt tttctacatc
      661 tacagcgtag gcttttcctg aaacagtttc ttcataagtc caatgccaac tttgctccca
      721 actggaagca atttcggatt tatgcacaga ttggatttct gatactgcag gaagtctcat
      781 tcggagactt tcacactttg cttttgcaga gtcccagtcc atttccccca actcgtcact
      841 ccactgctcc tgtttttttg cggagttttt ttctgtatcc aagtaagtct tctgtttaaa
      901 aattatgata tcttctttct cacttatcct gacaggaata tcactgtctt catctctaat
      961 atggcagtcc gataaggcaa aaatgaagac aaagcagaac ggcattcttt tcattcagtc
     1021 cccctctgat gaaagttatc atttccaacc agtctcatat gcagacgttc attctccata
     1081 aaaaaatcgg tgtctgaaca cctgcatctt gaatatccgg aatcagtaat caccttacgc
     1141 ctgcgaccgc gcggcgtttt gggcaagatg agtcagtaat tcagaattca gtcaggctgc
     1201 aatatgattt tttcattgca gattctgaga gaatcctgca gaatgaaact tatgaagtca
     1261 aaaattatat cattaatgat tatctcaagt ctgatatact gttcctccga gcagatcagg
     1321 gaaaaaccga tttctccttt tctggacact cagggacaca ggggtgcaag aggactgaaa
     1381 ccggaaaata ccatcccagc attcgaaact gcaatgcagt acggaatgcg cacaattgaa
     1441 ctcgacacag tcgtgacaaa ggacaagaaa ctgattgtcc accatgacac tgaaacaaat
     1501 ccggtgatct gcagaaactc cgacggaact ccgattgttt cccgaaaaat aagtgagctt
     1561 actgtaagtg agcttaaaga tctggactgc ggatcgcctg taaacccgaa attccctgaa
     1621 cagaaaccgg ttcccggcac aaaactttcg actctggatg aattctttca gtcagtgaag
     1681 gattatgaaa aaaagaataa gataaaaact ccgtttctgt tcaacgtaga gacaaaattt
     1741 gataaaaatc cgactgaaga agaaacaaag gaatttgcag cactgatgac tgattatatc
     1801 agaaaagccg gagtcactga cagaaccaca gttcagtcat ttcatcttcc tgttcttccg
     1861 gaggtgagaa agattgaacc taaactgaaa ctttctgcac ttgtggaacc gggatacttc
     1921 ggaggactca ggatgtatct gggactggga gccggagttc ggaaatcggc tatactgaaa
     1981 gcagaggaac tgaaggtgga tgttctgtct ccgtatcatt tgtatgtgac cccggaattt
     2041 gtcagagagg cgcacaaaag aaacattcag gtgattccct ggacggtaaa tgaaactgat
     2101 ctgatgaagt ctcttctgga aaagggcgtg gacggaatta tttccgatta tccggacaga
     2161 ctttctgctg cagtcgaaaa aaaataaagg aacaatccac ttgactgcat atgtgtaccc
     2221 gtcaaataag tagaatgcgg gagcttttca aatggtcatc aggaaacaga ttctttggag
     2281 cactgtatct gtctgcactg atccacttcg tccttcttgt ccttgtgctt tctccgattt
     2341 ctgaatatga tccagttttc tgcggatacc atatactcaa acttgttccg aaggaaaaat
     2401 ctgcactgga gtttgaattt ggttcttctg aagacggaaa ttccagcctg gagaaaggct
     2461 cacagcagca gccttctccg cctactggga atccttccag agaagaagga aaaagtctcg
     2521 cagaaaatca gggaaaaatc aaggagaaat acgaaggaac cgagtggaaa gatcttgtag
     2581 agaatctgga aaaaacaaaa gaccttcgta aaaacttcaa ggaagatttt cagaacaata
     2641 tcctccagaa cggcggtgcc actgacagat acataaaacg gtacaggcac tatgaggata
     2701 tggttgtcaa ggaagtgctg cctactttgg gaagcattga tgagccgttc tcaaaaaacc
     2761 tggacaaggc ggatgaggtt ctggaaaaac acagagaacg gaatgaaatc atagaaaaat
     2821 tccgttcagg tgacggagat gaaccgacca aagtggagat tgccaaaaaa agtccgaacc
     2881 gttccgaaac ccctctcaaa atgtccgaag aagaaatgaa tcagtatctg gatgaaactc
     2941 tgaaaaatca taaggaagag cagatggaag aattccgtca gaaatatgcg aacggatatg
     3001 accctgacaa aggagatctt cccaagcttt tcagagaact gtattacaag aatctgagaa
     3061 ggattgcata caatttcagt cctgacccga cttattttgc tgctgactat tttgaagaaa
     3121 atctgaacaa ggaagactat cttcggaatg cgatggagct tgtcaaaaaa tggaaaggca
     3181 ctaagacggc tactgaaata cttttcacta tttttgatat atacagtatc caggaaaggg
     3241 ctgtaggcga ggttcttcag atgaagactg tcctgaaaaa tcagaattcg gcagataaaa
     3301 atcagatccg ttcagagacg atcagaagga tcactgaaaa gtatgatcct gttctgacaa
     3361 agaaaggaat aaaaaacagt gccgatgctt caaaactcta cgatgcaaaa aagctggaaa
     3421 tactgaatta tattctggaa acaagcccgg aagggtacag aaaaaatgat gcactgtttg
     3481 aaaaaggaag agtgcttttt gaaaaatcat acagatcaaa ttctcaaagt tttgaggaag
     3541 ccgctgaggt atggagaaaa atccagcctg aaaaggacaa agaattcata ttctccggct
     3601 cctacgaaag acttaagagt gcactgaagg aaatcgaaaa gaatccttcc tcggcaggaa
     3661 ttatgagaat gcaggttctg aacattcttc agatgagaga agcagaatcg atagaaaaga
     3721 aaagagaaag agaacagaga cttctctggg gaaactgaca tcattatgaa gaaagttatt
     3781 ttaattactg gctcaacaga cggcataggc aaaaatacag cccgcattct tgcctcacag
     3841 ggacataaaa tcatactgca cggcagatct gaagacaagt gcctttctgc aagaaaggaa
     3901 ctgctttcag aaaatcctaa ggcggaagtc gactttgctt caggagacct ttcttcaatg
     3961 aagcagactg ccagacttgc agatactgta atgaaaaaga cggacagact cgatgtgctt
     4021 ctgaacaacg caggagtatt tatgaatgag aaaaaacttt ctgaagacgg gtttgaactg
     4081 agttttgcag tcaatcatct ggctcatttt cttcttacaa tgagactttt ggacttcctt
     4141 aaaaagaatt caggattcag aattgtaaat ctgagttcag tggctcacag aaacggtgta
     4201 tttgatccgg aaaatcttca ggcagagaaa aacttcaccg gatacggagc atatgcactt
     4261 tccaaactgg caaatattct ttttactatg gagctttcgg agaaaatcaa aggaaaggga
     4321 acttcaaact gtatgcatcc tggagtgatc ggaacaaaac ttctgaagac tggattcaat
     4381 atgagcgggg gaactcttga tgaaggaagc gacaattccg tatacctttc cgtttcaccg
     4441 gaagtgcagg aaattacagg aaaatatttt gtgaaaagag aagtcagtcc tgtcaaccca
     4501 aaactgattc cgaaagaaca cagaaaacag ctttgggaaa tcagcgaaaa aatgacatct
     4561 gagtttcttt gattacggcg aaaagggaaa gctaacagag tcctccccga tatttgatca
     4621 ttatgatcca tgatcaaata tcacatccat gatgattact tttttatcgc tgttgacctc
     4681 cgatctcaag tacatctcat accagttctc gaacttatga cttctcaggc ggaacagtgc
     4741 atcaataaag tcatccatat ccgtcttctt tgtcttaaac tccaccttgc cggggaagtc
     4801 cataatgatt cttgtgtggt cgcttcccct gtgatcttca ctgaaacttt catttatcag
     4861 aatcaacggt ctggaatgga gaatactgtt tttgccaaat acttttccga tctcaaacag
     4921 cagaggattt gatctttcat caaaattctt accaacccag tcgatattgt attttatttt
     4981 ctttcctttc ttaggcttaa cacggacaaa ttcttcgcct tcttcaaact cacattcatc
     5041 gtcgcccgtg cagcgggtca caggatcatg agggaatcct tcatattctg aaaaatacca
     5101 ttgattcggc tgattatgat actcctttcc gtcatattca aatgaataca gagcgtgcgt
     5161 ttcataaatg aatcctgcaa gagaactcat caatattcga agctcaaaat tatcgagaac
     5221 atccagatta aattcagact tcctggtgac tgcttttgag agaaccttcc tgagagcttc
     5281 cagctcttct tttttggcat tttccgattc cagctgctcc tgaagtgccc tagcctctgc
     5341 ttccgcaatc atctttcttt tctgtgccgc tttagtattc tttttcttct tcgaggcagt
     5401 tttctttttt gcaatcgttt tctttttcac tgctgttttc ttttttttca tacctgtacc
     5461 cttgccgtct ttttagaaat ttctgacgaa cgcttgtata tctgcctcaa aaaatgtcaa
     5521 ctttttcaga tacatccggc agatatacat actgcccgca gttcaaggct cataatattt
     5581 aatcattatg atccatgatc aaacatcaca tccactgtga ttgccttttt atcgctgctg
     5641 acctcggcac cataatacat ctcataccag ttttcaaact tgtgacttct cagccgaaac
     5701 agagcatcaa caaaatcagt catcttcgtt ttttttcctt tcagtgccac cttgccgggg
     5761 aagtccagaa tgattcttgt atgatcactt cccctgtgat cgtcattgaa attttcattt
     5821 ataaggatca acggtctgga atgaagaata gggttttgac tgaatacctg accaatctcg
     5881 taaacaagag gttttgactc ttcatcaaat tcccgtcctt cccagtcaat attgtatttg
     5941 atctttttcc ccggcttcgg ctgaacccgc acaagtttct gaccttcttc aaactcacat
     6001 tcatcatatc ccttgcaggg agttacagga tcatgaggaa atccgtcaaa ttcaccgaaa
     6061 taccattgat tcggctgatt atgatactcc tttccgtcgt attcaaatga aaaaaaagta
     6121 tgagtatcat atatgaatct gatgagtgca ctcagcagta tcttaagctc tgaatcatcc
     6181 agaacatcca gattaaactc agacttcctg gtgactgctt ttgagagaac cttcctgaga
     6241 actgcctgtt cttctttcct ggcattttcc cattcctgct gctccttcag tgccttagcc
     6301 tctgcttccg caatcatctt tctttcttct gccgctttgt tattcttttt cttcttcgag
     6361 gcagttttct ttttcactgc tgtcttcttt tttttcatac catacccctt gccgattttc
     6421 taccggacac ttgtatatct gcctttaaaa aagtcaattt tttccaagaa aatccggaga
     6481 atattcatag tgatttcggc tcaagcgaat ctctgtatgg ctccagaaat accggacatt
     6541 cagaatgcaa tataactatg atccattatt aaatatcaca gtcaccagga tggacttctc
     6601 atcaagtctg acctcagctt ctgaatacat ctcataccag ttctcaaatt tatgactctt
     6661 taggcgaaag agaccatcta tgaaatcatt catctct
//
LOCUS       DAOQGJ010000010         6627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_5697_length_6627_cov_3.223068, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000010 DAOQGJ010000000
VERSION     DAOQGJ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5697_length_6627_cov_3.223068"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK683_00330"
     CDS             complement(<1..546)
                     /locus_tag="PK683_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNH06916.1"
                     /translation="MDLPKAKKKNSTALIVEGGGMRGAFAGGALSAMCKYYPSKNFDL
                     MLGVSAGSCSLAYYATEDRRSFPSLKKILEIWQNELHGSRFISFKNLLRGSTILNQKY
                     LIDYLIADKHRINIEKFKRKLKIPFYIAVSNLENIKAEYIKVNHENIFQVLKAATALP
                     IATKGRHLLNQIRYSDGGITDP"
     gene            complement(883..1029)
                     /locus_tag="PK683_00335"
     CDS             complement(883..1029)
                     /locus_tag="PK683_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06917.1"
                     /translation="MEDRKTLYSWLGLAPNNKISDDKILQRGTVKLKSRAASLGLSIV
                     QSPS"
     gene            2065..2931
                     /locus_tag="PK683_00340"
     CDS             2065..2931
                     /locus_tag="PK683_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06918.1"
                     /translation="MEIYMKTKIIQLKFGKYVFSTASFLLTFFLISNISCGSEKKTFV
                     PYALIGINAAKSNNSSSGTGADNASNGSGVGSTSTSTSASTGTAAISCSDGIKNGTET
                     DIDCGGSCSSKCGTNKSCNLSAECQSGVCTSHLCQAATCSDGIKNGTESDIDCGGSCP
                     SKCGTSKTCSFSADCVSGVCTGNTCQAPTCFDTVKNGTETDSDCGGSCAAKCGYLKSC
                     SSGTDCSSNTCVSNTCIALQCTDMVKNGTESGVDCGGPTCLKCGSGKQCNSNSDCSSS
                     SCYFDVSGNFCL"
     gene            2949..3200
                     /locus_tag="PK683_00345"
     CDS             2949..3200
                     /locus_tag="PK683_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06919.1"
                     /translation="MNSPYAVTVDSLNGVYIADTGNNRVLYYPPNSTAASLVFGQTNF
                     TGNAPGTTATTLNTPRGVAVDSANRLYIMDNVNSRVLGY"
     gene            3477..6173
                     /locus_tag="PK683_00350"
     CDS             3477..6173
                     /locus_tag="PK683_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06920.1"
                     /translation="MFFAQNTPARFVSSDFISNSTLAPVVIAKNRKLGFTMDAAMSTS
                     CELTFNGTVYSSTVTLSTDKKTIYFTPNSSGWPVKLDLVTSINSNSCIDGAGNRLPTQ
                     GTGLPVYIADTVTYASASGSDSGTGTDTDPFLTLGKAMSSASASCPGACAIAMKGGTY
                     TVSSSVLMPANISIFGGFDPADWRIRRADKTMLSPYDTVFEDSSVNVSGLATSPYSTF
                     KFNSFTGTAEKSILDGIVIKGPVSGTAFNFGGPLGFANLPAGSGFTVRNTVTYDRSGS
                     ANFSSAGFIGAANSGKIILYNSSFSGSGDTLAVSSSRYGIYYNGSSAGSDLTVSSCDL
                     FGGAATLSTAGFFPSGTVSGKITLYQNTVKGGNNSGGDSTGIGIAFSGTDGFTAAENT
                     VSSGTGTVNGYAIYHTSGTGAVISKNKITAGNGMTTSAGIELAGTCSNAVINENEIIS
                     TGSGGALIGIRSSVPGTTHILSGNKINFGLWNGSTVKGMIVSGSSQVASNTVTLSGCS
                     GTSLNSTGIESNATFPVYVSNIVSSGDCPNGTSYGFSTASGGGSISISSNSFTSGSGL
                     SESVGIRFSINQGVTLTGNTVSAGTCTGASCFNAGFYSTGSTSGTTLTGNSLTVAPCT
                     GNTCIQSAMQHLSTGSPILTENVFNGGNSVMSTAVVRALNLSAAGGTLKRNTFTVGSG
                     NANMRTVDLGNTASSLKMCANVLVGGTSTSAGNPTTLAIANLSNAAVQFYGNTFIGGQ
                     SAGPVDIVAFTSAGAYSFDFKYNLLSGAPGYAGNTNCMNETAAVTYSALDTDNFSTCN
                     TLYLNMPGPVLMTQICAGSIGDGTCTSTLSASPAASNNTNLTPVFSSPASIDYHLSTG
                     TPAGLTTALGAPALASITGSCGDQLDRDGASRTNPSSIGAYK"
BASE COUNT         1896 a         1430 c         1350 g         1951 t
ORIGIN      
        1 cggatcggtg attcctccgt cagaataacg gatctgattc aggagatgtc tgccctttgt
       61 ggcaatcggc agagcagtgg cagctttcag aacctggaat atattttcat gattgacctt
      121 gatatattct gccttgatgt tttccagatt ggaaactgct atataaaaag ggattttcag
      181 ttttcttttg aatttctcta tgttgatcct gtgtttgtct gcaatcagat aatcaatcag
      241 atatttctga ttgaggatag tgcttcctct gagcaggttc ttgaaggaaa tgaatctgct
      301 tccgtgaagt tcgttctgcc agatttccag aatttttttc aaagagggaa aacttcttct
      361 gtcctcagtt gcatagtatg cgagggaaca ggaaccggct gatacaccca gcatcaggtc
      421 aaaattttta gaaggataat acttgcacat cgcagaaaga gcccctcctg caaatgcacc
      481 gcgcatcccg ccgccctcaa caatcagtgc agtagaattc tttttttttg cttttgggag
      541 atccatttct aatttactcc agagttaaaa cttaactgta tcaaagtgct tctttgcaga
      601 tgaagatgtc accttgagct tgcctaaggt tttcttcaac gaagacaagg gcagactcgc
      661 ggcacttttt aaaaacggat ttgatcgcaa tacgctttta tatatcatca cagacgctta
      721 tgcaacagtt ttttgacttg gatacaaagt caatattata tgttttccat atcataaacg
      781 ggaaggttta attttaattg aacagtgttc agtttgcagt aatctgctca ttgagacttg
      841 cagtataact caaatttctc ctaggaacaa gatcttatgg aatcatgaag gactctgaac
      901 gatcgagaga ccaagggaag cagctctgct ttttaatttt actgtccctc tctgaagtat
      961 tttatcatcg gatattttgt tgttcggtgc aagtccgagc cagctgtaaa gtgtttttct
     1021 gtcttccatt tgctcatacc cggaccgact tccgaaacca cagtcagtgc agtaagggtg
     1081 ttgaatctgt ccacctgtgt caggtcagtt cctagcattc tgtcaattgc cttcactgcc
     1141 attttcctac agaagccaga taccttctga tgtccgaaag ttcggaatca tatcatacac
     1201 ttccgcaccc agacttttcg aaaggccgga aagcagagtc agcacaacct tcggaaaatc
     1261 cataattctg ttccttgtga atgcagtctt atctgttctg aatatttcct ttcaattcct
     1321 taacctgaca gcattggccg ccgagtgcaa tgctgtcaag tcagggtcac ctgtaaatga
     1381 tattgtgctt tctgaaaatt acagcgtcta ggcagcatat cgtattttca tatgactatt
     1441 cagtattttt ttatcaagcg gttcccgcac agacagacag taggatcagg aagctgtatt
     1501 tgaacagata aaaccttcag aagactctct gtatgaaaat aaagcttccg cttaaggtga
     1561 gtgaaaaata atgaatatga tataatttat tcacaaaaca gacattgaag taatccgaat
     1621 aatccgcaat ctcatatatc tttttatttt caactttatt attaatttta agtgaatgct
     1681 tccgatcctg ccgaaaaaat gattgcagac tgaaaattta ttccttataa tgatcttaat
     1741 gagggaggca aatggttatg ctcttgaatc ttaatcgcag aaagaagaca gggattggaa
     1801 tcatactcac tttaatatct gcaggctgtc ttcagaagcg tacttcactg cttttcaata
     1861 ctaatcatat catctaatgc agatttttca ggaaattgat tttcattttc agaaatacct
     1921 tttgtttgct ggttattatc tttaggaagc ctctattaac catctaaaat gggagttttt
     1981 cgaggttctt tttagttttt acaatattat tattgacatt aatgtataat actagaaaat
     2041 aatctataat caaatatttt atgtgtggaa atttatatga aaacaaagat aatacagcta
     2101 aagtttggga aatatgtatt cagtactgcg tcttttcttt taactttttt tttgatatca
     2161 aacatttcgt gcggaagtga aaaaaaaacc tttgtacctt atgctttgat aggaataaat
     2221 gccgctaaat caaataacag cagctcaggc actggggcag ataacgcaag caatgggtca
     2281 ggagttggaa gcacttcaac gtcaacttct gcttcaacag gcactgccgc tatatcatgt
     2341 tctgacggca taaaaaatgg gacagaaacg gacattgact gtggaggttc atgttccagt
     2401 aaatgtggta caaataaatc ctgcaattta tcggcagaat gccaaagcgg tgtctgcaca
     2461 tctcatcttt gtcaggccgc aacctgttct gacggcataa aaaacgggac agaatcggat
     2521 attgactgcg gaggttcatg tccttcaaaa tgtggaacgt caaagacctg cagtttttca
     2581 gcggactgtg taagcggtgt atgtactgga aatacctgcc aagcccctac ctgttttgat
     2641 actgtaaaaa atggaacaga aacagacagt gactgtggag gttcgtgtgc agcaaaatgc
     2701 gggtatttaa aatcctgctc ttctggaact gactgctctt caaatacctg tgtaagtaat
     2761 acttgtattg cactgcaatg tacagatatg gtgaaaaatg gaacagagtc aggtgttgat
     2821 tgcggaggac ccacctgcct gaaatgcggt tccgggaaac agtgcaactc taattcagac
     2881 tgcagcagct catcctgtta ttttgatgtc agtggtaatt tctgcctctg actatatcag
     2941 cttccacact gaattcacct tatgcagtga ctgtagattc cctgaacggt gtttatatcg
     3001 ctgataccgg aaataataga gttctttatt atccaccgaa cagcacagct gcttcactgg
     3061 ttttcggtca gaccaatttt acaggcaatg caccgggaac caccgcaaca actctgaata
     3121 cacccagagg tgttgccgta gactctgcaa acagactcta tataatggac aatgtgaaca
     3181 gccgagtatt aggatactag aattcggttc acatctgtcg gcattcaact gtatcggaaa
     3241 tctaaaattc agtacgtaat atatatgact ccccgcttgc tgaattactg attttatttc
     3301 cgttcaaaaa aatttctgtc tcctgtttaa aatgattttt ttgatttaca gtcaggcaat
     3361 tcctgcctat gcttagaact atgaacagga tactgtctgc cttcgttctc ttgatttttt
     3421 ttcaatgcag tacacgattt gaccgaaaaa tactgcagta ttccggcata ttgggaatgt
     3481 tttttgctca aaatactcct gctagatttg tatcttccga tttcatatcc aattccaccc
     3541 tggcaccggt tgtcattgcc aaaaacagaa aattgggatt tacgatggat gccgcaatga
     3601 gcacatcctg cgagctcaca ttcaacggca ccgtttattc ttccactgta actctctcca
     3661 ctgataaaaa aacaatttat ttcactccga acagttccgg ctggcctgtc aaactggatt
     3721 tagtcacttc aatcaattcc aattcctgta ttgacggggc agggaacaga cttccgacac
     3781 agggaaccgg actgcctgtt tacatagcag acacagtaac ttatgcatct gcttcaggga
     3841 gcgacagcgg cactggaacg gatacagatc catttcttac actcggaaag gcaatgtctt
     3901 cagcttccgc ctcctgccct ggagcctgtg caattgcaat gaaaggagga acatatactg
     3961 tatcctcttc agtcctgatg cctgccaata tcagcatatt cggaggattt gatcctgccg
     4021 actggaggat aagaagagca gacaaaacaa tgctctctcc ttacgacact gtttttgaag
     4081 acagttctgt aaacgtttcc ggactggcca catcgcctta cagcacattc aaatttaatt
     4141 cttttaccgg cactgcagaa aaaagcatat tggatgggat tgtaatcaaa ggtcctgttt
     4201 caggaacagc tttcaatttc ggcggtcctc tgggttttgc caatctcccg gcgggaagcg
     4261 gatttactgt cagaaataca gtcacttacg acagaagcgg ctctgcgaat ttttcttctg
     4321 caggatttat cggggctgcc aattcaggaa aaatcattct ttacaattcc agcttctcag
     4381 gatcaggtga cacacttgca gtctcatctt ccagatatgg gatatattat aatggctctt
     4441 ctgcaggaag tgatctgaca gtgagttcct gcgatctttt cggaggagca gcaactctga
     4501 gcacagccgg attctttcca agcggaacag tcagcggaaa aattacactt tatcagaata
     4561 cagtaaaagg cgggaacaat tcagggggag actctaccgg cattggaatt gctttttccg
     4621 gcacagacgg atttactgca gcagaaaaca ctgtttccag cggaactggc actgtaaacg
     4681 gatatgccat ataccatacc tcaggtacag gtgcggttat ttcaaaaaat aaaatcacag
     4741 caggaaacgg tatgacgaca agcgccggaa tagagctcgc aggcacatgt tccaacgcag
     4801 tcatcaatga aaatgaaatc atctccactg ggtcaggagg agcactgata ggaatacgat
     4861 cctcagtgcc cggtaccact catatattat caggcaataa aatcaatttc ggtttatgga
     4921 acggttcaac agtaaaaggt atgatagtaa gcggatcttc tcaggtcgca agcaatactg
     4981 tcaccctttc aggatgctca ggaaccagtc tgaattctac aggaatagaa tctaatgcca
     5041 cttttcctgt ttatgtttcc aatattgtca gctcaggcga ctgtccgaac ggtacatctt
     5101 acggattctc cactgctagc ggaggaggaa gcatcagcat cagcagcaat tcattcacat
     5161 ccggttctgg actgagtgaa tctgtaggta tcagattttc gatcaatcaa ggagttactc
     5221 ttacaggaaa cactgtaagt gcgggaacct gcacgggagc gagctgcttt aatgcagggt
     5281 tctacagcac aggctctaca tccggcacca cactgactgg aaacagtctt acagtcgcac
     5341 cttgtacagg aaatacctgc attcaaagtg caatgcagca tctttctacc ggttctccga
     5401 tattgactga aaatgtattc aatggaggaa attcagtgat gtccacagct gttgtacgag
     5461 ccctgaacct gagtgcggcc ggaggcacac taaaacgcaa tacattcacc gtcggttccg
     5521 gaaatgcaaa tatgagaaca gtagacttgg gaaatactgc aagctctttg aaaatgtgcg
     5581 caaatgtttt agttggaggc acaagcactt ccgcaggaaa tccgacgaca cttgcaattg
     5641 ccaatctgag caatgcggcc gttcagtttt acggaaatac attcatcgga ggccagtcag
     5701 ccgggccggt ggatatcgtt gcatttacat cagcgggagc ctattccttt gattttaaat
     5761 acaatcttct ttccggagca ccgggatatg caggcaatac aaactgcatg aatgaaactg
     5821 cagcagtcac atattccgca ctggatacag acaatttcag cacctgcaat acactgtatc
     5881 tgaatatgcc cgggcctgtt ctgatgacac agatctgtgc aggcagtatt ggcgacggaa
     5941 catgcacatc cactctttcg gcctcccccg cagcatccaa caataccaat ctgactcccg
     6001 tattttccag ccctgcctcc attgattatc atctgagcac aggcacgcct gcaggactga
     6061 caactgcact cggagcgcct gcactggcaa gcattacagg atcctgcgga gaccagctcg
     6121 accgggacgg ggcttctaga acaaaccctt cttcgatcgg tgcttacaaa taaaggatgg
     6181 gaagatgtca atttcatctc ataaccgtat tcagcattcc agcagatgat atgcttcctt
     6241 agtaataatt ttttatactg tctaattgta ttatcatttt ggactttttt cactgcctat
     6301 tctttagctg cagaaaaatg cagatcagta aatattttcc aaatcgagtg ttttattgaa
     6361 aacactttat tttcagggtt tttattgttt tcaactgcag gaatttcatc tgttctgtcg
     6421 gcatttttta taacacatat cattatgaat atgaacggga ggaaagattc agtggtacac
     6481 agcatacttt ttacatttat aatcatattt actgtaagat ttttttatac aatgaatttt
     6541 tctgaaacag gctggagacc gtttttcatc ggatatcttt tgactctcat ttcctatgca
     6601 atacttcaat atttcagaaa gaaactc
//
LOCUS       DAOQGJ010000011         6499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_5847_length_6499_cov_3.369181, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000011 DAOQGJ010000000
VERSION     DAOQGJ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5847_length_6499_cov_3.369181"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..2470)
                     /locus_tag="PK683_00355"
     CDS             complement(188..2470)
                     /locus_tag="PK683_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNH06921.1"
                     /translation="MDRKFLQYSGVLGISSSGAAPSGLNYPDSPYSFTVNVSVGKKTP
                     TVTGSVTDCTSNPALPTGLSIDRMSCTISGTPTVLQSQTKYTVTAYNSFGSTSTAVSI
                     GVFTSVNVQFSFTSASLSDSGSSLTLSAFGGPTSVNGKDGDTNGAYNFDGSSQYLSIT
                     NPSGLPTGSSPRTICAWANPRSFPAVSGTFIIANYGSATASSSFGLSMMNSAGTNQVG
                     ITGYNDDLSANYTVPLNAWTHICGVYDGTTASLYIDGKLFSSGAKSYNTGSSFLFIGA
                     QSSLTQHFNGKIDEVRIYNTALTAAQIRQITVQVPSGLAARYDFNGNPAEVSGSGISL
                     SSSGSFALTADRHGIAGNAYTFNGASSFSTAAPITVSNTGNITITAWIRPTNFNGAVN
                     MIIVNGNGSFNGYGIYINGTASNVLTGIIGGVANVASSIAPPLNVWSHISLRATGTNW
                     DLRLNGAPIGTLASAAPITPTVGTFIGSSPTNEFFTGDIDDVRVYNRALTDSEILVLA
                     GYHPMQVSTWTATPATSSFKIHFQADSLSSLANGATVTTWNDNSGNGNHISAGVSPTY
                     SSAGMNSKPAVNFARASTQYLTRAAAVGLGGDNFSMFSVFNPTIIGGDMGIFSIGSTC
                     GYDKEQMISDITNYLQAGVCNVISIGASTNPVSSGISYIHSFTYQISSTGNVYLNGTS
                     VVSMGTPATSYLGNMDIAVGRRMNDYTLAPIANTKYFNGLISEAIYYDGSLSNANRIL
                     VECYLSSKYNIVLDPGIICP"
     gene            2778..3656
                     /locus_tag="PK683_00360"
     CDS             2778..3656
                     /locus_tag="PK683_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06922.1"
                     /translation="MHNALSQVSVVCGPYTLKGFSISGLATYVQVPELDICFDMGECP
                     VSAVPIRHILLTHAHGDHARCLLRHRALRGMLGIQGEAVYIIPEPIIKKFNDLAKADA
                     LFEGVTEEEYIPPNTVSLNGGESLYELPFRKGIFIKSFTVEHRGIPSLGYTVMNRRKK
                     LKQEYLNLPGQEIARLRKEGIEINDESDVPLITFIGDCTVESLYKEKHIWKSPILMIE
                     ATFVDPSDRELAGKKGHTHIDEIGEVLREFQKEELPEHIVLKHFSMKVSKAEIAAAVE
                     RSIPDSAKSRIRIFLD"
     gene            complement(3917..4117)
                     /locus_tag="PK683_00365"
     CDS             complement(3917..4117)
                     /locus_tag="PK683_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06923.1"
                     /translation="MKKKEDRNTRYYIDLDLKTGKIIQLDCDQKEGLAQELPLPHQQR
                     VFLTKGQYNKLLKKYSELKNSP"
     gene            complement(4163..4996)
                     /locus_tag="PK683_00370"
     CDS             complement(4163..4996)
                     /locus_tag="PK683_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06924.1"
                     /translation="MKEYRFNHDAETVELASEYMYIRSKQFLKYFYRFERLETYDWGI
                     SAFFSKKGKFFQSIYLLKQFRGRGLYSKNVKETILTTAECGIEEYLKNNKIPYQMEKA
                     VPFREYEMISEFYKDRKAVRSDVYLMNHIDEGLFVLEKIGASDTAKRAYVLHPLVQND
                     DSLPENYETVFTENVSDTKTIIAMMEYRSVANEYLSKRKISSIEEIRLSPLKDVNDML
                     IADKIQNRKDFEIFHKGKHERSAELEDYFSNWLKRLNISEEFYKECYDFLHQEPVIHS
                     K"
     gene            complement(4993..>6499)
                     /locus_tag="PK683_00375"
     CDS             complement(4993..>6499)
                     /locus_tag="PK683_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH06925.1"
                     /translation="IGNFGMTFAIETIGQALLNASDHGKYDFIMFSDEGTVIYHKNYA
                     NLNKNFKKDFSGESYSGFESLIGENFEGVDRYNRQFHDIVFEGVRMKGMTVPLPKFNS
                     RLLILIPKNEFYVKLNITLLQISFFIILFTVFLTLIIRGITNNFTVPIRDTIQIFEKI
                     SKGDLSVRPKDFVPDEFGEIIRYLHSLLNVLRDTVSVIQSSSEKLESTSEVLSRTTVS
                     MAATTQKQSKSLNESAQFLQEISGSVEKVAENSKLAFHSSKHTFSSMEDLLIKVKEVQ
                     EHSHQAKAIALISSKEAQKGNELMKNAIRGMENIDSSTKKIAEIVGLISDISKQVNLL
                     ALNAAIEAARAGEFGRGFTVVADEISKLASKTSNSAKSIREYIEEGLKEVAYGKEYVD
                     QTAKALSHIIESIEKNNSIIEMITEASRIQTESSEAVLTDVKEVMEMAESIHRATDVQ
                     AKSSQELTSNVSHVADLTDFVAKGADEVADTSQKILEQTKSLNDQVDFFKI"
BASE COUNT         1752 a         1371 c         1382 g         1994 t
ORIGIN      
        1 atggattttt gctgtaaagg aatttaaagt tttctatcag aatatagttt ctattatttc
       61 tgatattcag tctcaaaaga aattcttttt tgtattctca gaatccggtc atcttctgtg
      121 aaagttccat ttggaaagaa gctgagggtt agacagaata aaaattcatt attattacat
      181 gatttttcta gggacagatg attcccggat cgaggacgat attgtatttc gaagaaagat
      241 aacattccac cagtatccgg ttggcattgc tcaatgaccc gtcataatag atcgcttctg
      301 agatcagtcc gttaaagtac tttgtattgg caataggagc cagtgtataa tcattcattc
      361 ttcgtccgac ggctatatcc atatttccca gatacgatgt ggcaggggtt cccatggata
      421 cgacggatgt cccattcaga tacacatttc cagtactgct gatctgataa gtaaagctgt
      481 gaatataaga aattccggaa gatactggat tcgttgatgc accgatggaa atgacattgc
      541 agactcctgc ttgaagataa ttggtgatat cggaaatcat ctgctccttg tcatatccgc
      601 atgtactgcc tatcgagaaa atacccatat ccccgccgat aatcgtggga ttaaaaacac
      661 tgaacatact gaaattatct ccgcccagac cgacggctgc agctcttgtc agatactggg
      721 tagacgctcg tgcaaagttc actgccggtt tggaattcat acccgcagaa gaatacgtag
      781 gagatactcc agctgagata tggttcccgt ttccgctgtt gtcattccac gttgtaacgg
      841 tcgcgccgtt ggctagactg ctcagactgt ctgcctgaaa atgaatttta aaactgcttg
      901 tcgcgggagt cgctgtccat gtgctgacct gcattggatg atatcctgcc aataccagta
      961 tttcagaatc agtcaaggcc ctattataca cacgtacatc atcaatgtcc cctgtaaaaa
     1021 actcgtttgt gggacttgat cctataaaag ttccgactgt gggagtgatc ggagctgcag
     1081 atgcaagggt tcctatggga gcaccgttca gtctcagatc ccagttagtg ccagtggctc
     1141 gaagggagat atggctccat acattcaaag gaggcgcaat agacgaggcg acattggcta
     1201 cacctcctat gatgcctgtg agaacattgc ttgcggtacc atttatatat ataccatatc
     1261 cgttaaaact tccgtttccg ttcactataa tcatattgac tgcaccgttg aagttggtcg
     1321 gtctgatcca tgcggtaatc gtaatattac ctgtatttga aactgtaatc ggtgctgctg
     1381 ttgaaaagct ggatgcaccg ttgaacgtat atgcgtttcc tgcaattccg tgtctgtctg
     1441 ctgtcagagc aaaagatcct gaagaactca ggctgatccc gcttccgctg acttctgccg
     1501 gatttccgtt aaaatcatat ctggctgcca gtcctgaagg aacctgaacc gtgatctgac
     1561 ggatctgtgc tgctgtcagt gcagtgttgt aaatccgtac ttcatctatc tttccattaa
     1621 agtgctgagt cagactgctc tgtgctccta tgaaaagaaa agaggaaccg gtattatacg
     1681 attttgctcc ggaggaaaaa agttttccgt cgatataaag acttgctgtt gtcccgtcat
     1741 ataccccgca tatatgggtc caggcattca aagggactgt ataatttgca gacagatcat
     1801 cattgtatcc tgtaatgcct acctgatttg ttcctgcact gttcatcatg gaaagaccga
     1861 aactcgagga ggcagttgca gaaccgtaat ttgcaatgat gaaggtgcct gaaaccgccg
     1921 gaaaggatct tggatttgcc caggcgcaaa tcgttcttgg actggatcct gtcggcagtc
     1981 cggaaggatt tgttatgctc agatactgag aagatccgtc aaaattatat gcaccattag
     2041 tatcaccgtc tttaccgttg actgaggtcg gtccgccgaa tgcagaaaga gtcagcgagg
     2101 aaccggaatc tgaaagagat gcagatgtaa aactgaactg cacatttacg gaagtgaaaa
     2161 caccgatgga gacggctgta cttgtgcttc cgaatgaatt gtaggcagta actgtatatt
     2221 ttgtctggga ctgcagaact gtcggagttc cgctgattgt acagctcatt ctgtcaatgc
     2281 tcaatccagt cggaagtgcc ggatttgaag tgcagtctgt gactgagcct gtaactgtcg
     2341 gtgttttttt tcctactgat acattgactg taaaactgta cggagagtcc ggataattca
     2401 gccctgacgg tgcagccccg gaagaagaaa tacctaaaac cccggaatat tgaaggaatt
     2461 ttctgtccat attaggcctg cagcagaaga atatactgca gaagaaaaag agacagatct
     2521 gtatcagttt tttcctctga ttttgtctga atttcataaa ttatttcctg cccgcagttg
     2581 acattatccg cttactttac ttatgagaat ccaattttgt caattgaata tgagagatat
     2641 tctgactgta ctgcataaaa ttgaactgct cagcctgatg tctttgctct gtcctcagca
     2701 gtgaaaggga taaaagctta tacagtaatt tcttcataga atttactgaa tctgcctcat
     2761 ctgaaatatg gttgattatg cacaatgcac tttcacaggt ttctgttgtc tgcggacctt
     2821 atacactgaa gggattttcc atctccggcc ttgcaactta tgtgcaggtg cctgaacttg
     2881 atatctgttt tgatatgggc gaatgtcctg tctctgcagt tcccatccgt catattctgc
     2941 tgactcatgc acacggtgat catgccagat gtctgctccg gcaccgggca ctcagaggta
     3001 tgctaggcat acagggagaa gcagtttata ttattccaga gcctataatc aaaaagttca
     3061 atgatcttgc aaaggcagat gcacttttcg agggagtgac tgaagaggaa tacattcctc
     3121 cgaatacagt ttcactgaat ggaggagagt cactctatga acttcccttc aggaagggaa
     3181 tattcataaa atcatttact gtagaacaca gaggaattcc cagtctggga tatactgtca
     3241 tgaacaggag aaaaaaactc aagcaggaat atctgaatct tccgggtcag gaaattgcaa
     3301 ggctcaggaa ggaggggatt gagatcaatg atgagtccga tgttcctctg atcacattta
     3361 tcggagactg cacggtcgaa tctctgtata aggaaaagca tatctggaaa tctcccattt
     3421 taatgataga agcaacattt gtcgatccct cggacaggga actggcagga aaaaaaggac
     3481 atactcatat agatgaaatc ggagaagttc tcagggaatt tcagaaggaa gaacttccgg
     3541 aacatattgt tctgaagcat ttttcgatga aggtatccaa ggctgaaatt gcagctgcag
     3601 tggaaagatc cattcctgat tctgcaaaaa gccggatcag gatttttctg gactgattat
     3661 ttttcctgaa agtcccgaat gacggactct gcttttatcc ggaaaatact gttttctgtc
     3721 cttccgcgaa gattcggaaa aagtctgact gaataactga gctgcgcaaa tacgatgtga
     3781 tgtggaaacg cagtaatttt cagaaagcat cagggaaatc cagcagttta acagcttgcg
     3841 atgaatcagt aaaaaagcat actagatttt cagcaggctt caaggctcca aactgccgac
     3901 aggtgaactg ttgtatttaa ggtgaattct taagttcaga atacttcttc aaaagtttat
     3961 tgtactgccc ttttgtgaga aatactctct gctgatgagg cagaggaagc tcctgagcca
     4021 gtccttcttt ctgatcacag tccagctgaa tgatctttcc tgtttttaag tcaaggtcta
     4081 tgtaatatct ggtgtttctg tcttctttct ttttcaaaag attccctcga ctgattttca
     4141 tccttcctct gaaaagcctg aatcatttac tgtgaatgac aggttcctgg tgaaggaaat
     4201 cataacattc tttataaaat tcctcagaaa tgttcagacg tttcagccag ttgctgaaat
     4261 agtcctcaag ctcagcactt ctttcatgct tgcctttgtg gaagatttca aagtcctttc
     4321 tgttctgtat cttgtccgca atgagcatat cattcacgtc cttcagagga gagagacgga
     4381 tctcctcaat tgagctgatt ttccttttgg agagatattc atttgcgaca gatctgtatt
     4441 ccatcatcgc aatgatggtt tttgtatccg agacattctc ggtaaataca gtctcataat
     4501 tttcgggaag actgtcatcg ttctgaacaa gaggatgcag aacataagca cgttttgcag
     4561 tatcggatgc tccgattttt tccagaacaa aaagtccctc atcaatatga ttcatcagat
     4621 agacatcgct tctgactgct ttcctgtcct tgtaaaactc ggaaatcatt tcgtattccc
     4681 tgaagggaac cgctttttcc atttgataag ggattttatt atttttcaga tattcctcaa
     4741 ttccgcattc agcagttgtc agaatcgtct cttttacgtt tttactgtaa agtcccctgc
     4801 ctctgaactg tttcagaagg tagatggact gaaaaaattt ccctttttta gagaaaaagg
     4861 cagatattcc ccaatcgtaa gtttcgagcc tttcaaatcg gtagaagtat ttcaggaact
     4921 gcttggaacg gatgtacata tattccgagg caagctccac tgtctctgca tcatggttga
     4981 atctgtattc tttcatattt taaaaaagtc cacctgatcg ttcagggatt ttgtctgttc
     5041 gagaattttc tgacttgtat ctgccacctc atccgctcct ttcgctacga agtcagtcag
     5101 atcagcaacg tggctgacgt tggatgtaag ctcttgactg ctcttcgcct gaacatctgt
     5161 tgctctgtga atggattctg ccatttccat tacctctttt acatccgtaa gaactgcctc
     5221 acttgattca gtctgaattc tggatgcttc tgtgatcatc tcaatgatgc tgttgttctt
     5281 ttcaatgctc tctatgatat gagaaagagc ttttgccgtc tggtctacat attccttgcc
     5341 gtaggcaact tccttcagac cttcttcaat gtattccctg atgcttttgg cactgtttga
     5401 agtcttggaa gcaagtttgc ttatctcgtc agcaactact gtaaagccgc gtccgaattc
     5461 cccggccctt gctgcctcta ttgcggcatt cagtgcaagc agattgacct gctttgaaat
     5521 atcgctgatc agacctacga tttctgcaat tttctttgtg ctcgaatcaa tgttttccat
     5581 tccgcgaatt gcatttttca tgagctcatt tcctttctga gcttccttgc ttgaaatgag
     5641 tgcgatagcc tttgcctgat gagaatgctc ctgcacttcc ttcaccttga tcagaagatc
     5701 ttccatggaa gagaatgtat gctttgagga atgaaatgcg agtttggaat tctctgccac
     5761 cttttccacg gaacctgaaa tttcctgaag gaactgtgct gactcattca atgacttgga
     5821 ctgtttctga gttgttgcag ccatacttac tgttgttctg gacagaactt ctgaagtgga
     5881 ttcaagtttc tccgatgagg actggatgac agaaactgtg tctctgagca cattcagaag
     5941 agaatgaaga tagcggatga tttctccgaa ttcatcgggc acaaagtcct taggcctgac
     6001 ggaaagatct cctttggata ttttctcgaa tatctgaatt gtatctctga tcggaacagt
     6061 aaaattattt gtaattcctc ttattatcaa tgtaaggaaa actgtgaaga gtataatgaa
     6121 aaatgatatc tggagaaggg taatgttcag ttttacataa aactcatttt tcggtatgag
     6181 gatcaggagt ctgctgttga attttggaag aggcactgtc attcctttca ttctgacacc
     6241 ctcaaataca atatcatgaa actgtctgtt gtatctgtcc actccttcaa agttttctcc
     6301 gataagactc tcaaaacctg aataactttc tccggaaaaa tccttcttga agtttttgtt
     6361 cagatttgca taatttttat gataaattac cgtgccttca tcagaaaaca taatgaaatc
     6421 atactttccg tgatctgagg cattcagcag agcctgtcca atcgtttcaa tggcgaaggt
     6481 cattccgaaa tttccgatc
//
LOCUS       DAOQGJ010000012         6499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_5848_length_6499_cov_2.809435, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000012 DAOQGJ010000000
VERSION     DAOQGJ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5848_length_6499_cov_2.809435"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="trpD"
                     /locus_tag="PK683_00380"
     CDS             <1..864
                     /gene="trpD"
                     /locus_tag="PK683_00380"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNH06926.1"
                     /translation="ETAEELFGFVRAVREAALPRTGIFDFDFLDTCGTGGDEKGSINI
                     STISALLLGSMGIRVAKHGNRSVSSLCGSSDFLESFGYNIHVPAERAEKRFFEKGFTF
                     LFAPVWHSSMKNAANVRKTLGFRTIFNLIGPLCNPLNPTHQIIGVYSSELLEKMILVL
                     KKQGTKKAILCCSKDGFDEFSLFSDTDYLLLEDGKISAHTFNISSLNLGTLKPSEIFA
                     ETKEVSIALSERIMKGENLSGTHAVALNAGAALFLFGKSESIEAGYKTAQAELLTGRT
                     WKFLQEVAEAS"
     gene            861..1484
                     /locus_tag="PK683_00385"
     CDS             861..1484
                     /locus_tag="PK683_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNH06927.1"
                     /translation="MMKYLFFGTCILHLLASGFFREYFGLSLLTKMIPMAVLLYSVLE
                     KGLSTKERKYFFIGLLFSSAGDALLAYENLFVFGLSSFLTAHLFYITGFSGSSGLHIF
                     RSLPFYISGAALGAYIIPIVPENLRIPVSVYMMVLMSMGWRAASRNANWISLKYSLIG
                     AFIFILSDTLIAFSKFLHMPMPPFPHFWVMVTYYTGQYLLWKGVDSV"
     gene            1494..3077
                     /locus_tag="PK683_00390"
     CDS             1494..3077
                     /locus_tag="PK683_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06928.1"
                     /translation="MKKTLRYKIFIFIFFGLLTVFSAALFQANSPRDIYFFTDILILP
                     AMYQDIIDGFPFSGWSFTPSPYFFPDLILYFPVRLLSGSFQASMYVYGMVQIFLLFSL
                     SVFIVRSMFTKTVFLFSAASGYVLISVCIILISEFRPFAIILIPTVHFGAYLILLACI
                     LYWLRNFSAGSVIPVLTVCAGVSDRLFHVIFSIPFALSCIFLWRYDSSSETEKSKPSF
                     IREFQAWKPAVRSILLSVLISVLVYNGLKSLIKLEAVSRVSPDISWKLFVQSILSLYS
                     KGGISAKILFAGFIPAYTFCFIYYIKKKEYLQRTVRFFLLFSLISVPVSLSAPVAAGT
                     FLDEYSFRYSVFSFFLLYCSFFLVLYVQFRFYLRRTKRRVLETLARISAYQFIFFSIL
                     MEGILIFMLDWERKMRDNFSYVPDEVKCAESILGNKIAVYGLSDYWNAKYFTLFSENR
                     LRVYQISYQDLRKNHTLNNKYWYEKGKSSRFGNPEYKFIITERLSEVLIEKKWGRPDS
                     VHLCSGKNVWIYRKGISLD"
     gene            complement(3069..4373)
                     /locus_tag="PK683_00395"
     CDS             complement(3069..4373)
                     /locus_tag="PK683_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNH06929.1"
                     /translation="MKNEWNLVLEEVSKQIPKVYFHPFIEPLLFEEYSDGKFFIKAPS
                     SAVKEHVEKKYQKFIEDAVFKVYGSSSAVEIFTEEQGKSIGEFIRQKFKEEKFLFNPD
                     FTFNSFQIGESNQLAYFASKEVISSPGKINPLFLYGKCGTGKTHLLHAVGQELQKNPN
                     ENVKYISISSFLSEFVMMVQNRQSLESFRNKYQSYTSIIFDDLQFLNITAEKTQEEFY
                     SLFNYMQERKRQIVLAADRPVMELPLQEGLRSRLLTGIQAEVKQADSSLRKKIISLKS
                     RECGISLSEESVDLISFLFPSDTRALLGAVNELSLYKRTYNLLILNEDKIREILESRI
                     TKSRAGDLSPDRILTEVSAYFEREKSDILSKSRRSEFILPRHISMYLIHELCEMSKSS
                     VGKLFKTQHTTVIAAIKKLESGMERDENLKKKVDDLRNKFLI"
     gene            4912..>6499
                     /locus_tag="PK683_00400"
     CDS             4912..>6499
                     /locus_tag="PK683_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH06930.1"
                     /translation="MSESLENLSHYIRNSFHSCVRGRLILPEIQGDFAEFPSSMNPLL
                     SKALNDLGIEKLYSHQKESFESVRKGNNTILLSRTASGKTFSFLLPILNDYAEKPGRF
                     TTLLLYPTKALSRDQESALGKILRSVSGDAKFGVFDGDTAREDRESLVKYADFILSNP
                     DMLHSGILPNHSRKWRNFLSRLKYIVVDESHIYRGAFGSHVSNVMRRLIRICRNYGTD
                     PIFICSTASIGNPAEHAKALFHKDFNLISNDGSPRAEKEILFLNPALVSRNGGPEYRQ
                     GPASISIPVICEAARMGIRTICFARARQEVERLYKAVAEKDSYIREKVKPYRGGLLPS
                     ERRALEKELFAGNLNTIITTNALELGIDIGDMSLCILNGHPGSVSSFWQQAGRVGRAG
                     KKSLILYIAKDMPIDQYIVHNKEFIISAGSEEAWLSADNPYIIMQHIPCAAYEIPFSA
                     EETIFSGPVFNLALSTLEEKGTLKKFREFYRYAEEEFPSKGVNLRGLTDYNVNIFWDG
                     KIIGEIDPIGAMGTLYKDAIY"
BASE COUNT         1802 a         1320 c         1323 g         2054 t
ORIGIN      
        1 gaaactgcag aggagctttt cggatttgta agagcagtca gagaggccgc tcttccccgg
       61 acagggatat ttgactttga ttttctggac acatgcggga caggggggga tgaaaaaggc
      121 tccatcaata tctccacaat ctctgctctt cttctcggtt caatgggaat cagagtggcc
      181 aaacatggaa acagatctgt atcctctctg tgcggatcaa gtgattttct ggaaagtttc
      241 ggttacaata tccatgttcc tgcggaaagg gcagaaaaaa gattttttga aaaaggattc
      301 acctttctgt ttgctccagt ctggcattcc tcgatgaaga atgcagccaa tgtcagaaag
      361 actctaggtt tcagaaccat attcaacctg attggtcctc tctgcaatcc tttgaatccg
      421 actcatcaga ttataggagt ttattcatct gaacttctcg aaaaaatgat tctggttctg
      481 aagaaacagg gaacaaagaa agcaatttta tgctgttcca aggacgggtt tgacgagttt
      541 tcactttttt cagatactga ctaccttctc ctggaggacg gaaaaataag tgcacatact
      601 tttaacattt catcactgaa tctgggcaca ttgaagccgt ctgaaatttt tgcagaaaca
      661 aaggaagtct ccattgctct gtctgaacgg ataatgaaag gagaaaatct gtcagggact
      721 catgcagttg ctctcaatgc aggtgccgca ctttttcttt tcggaaaatc agaatcaata
      781 gaggcagggt acaaaacggc tcaggccgaa cttctgactg gaagaacatg gaagtttctg
      841 caggaagttg ccgaggcatc atgatgaaat atttattttt cggaacatgc attcttcatc
      901 ttctggcatc cggttttttc cgtgaatatt ttggactgtc ccttctgact aaaatgatcc
      961 cgatggcagt tcttctgtat tccgtgctgg aaaagggtct gagcacaaag gaaagaaaat
     1021 atttttttat cgggcttctt ttttcctctg caggagatgc gcttcttgca tatgagaatc
     1081 tttttgtatt cggtctaagt tcatttctga ctgcacatct tttttatata accgggtttt
     1141 ccggcagttc cggtctgcat attttcagat ctcttccatt ctatatttca ggagccgcct
     1201 tgggagcata tatcattccc atcgttccgg agaatcttcg tatcccagtc tcagtctata
     1261 tgatggtgct gatgagcatg ggctggagag ccgcttcgag aaatgcaaac tggatttcac
     1321 tgaaatattc actgatcggt gcattcatat tcatcctttc ggatacactg atcgcatttt
     1381 caaaatttct ccatatgcct atgcccccgt ttcctcattt ctgggttatg gtgacttatt
     1441 ataccggaca gtatcttctt tggaagggtg tagacagtgt ctgaagcagc ctaatgaaga
     1501 aaactctgag atataaaata tttattttta tattttttgg gctgctgacc gtattttctg
     1561 cggccttgtt tcaggccaat tctccgagag atatttattt ttttaccgat atcctgatcc
     1621 tcccggcaat gtatcaggac atcattgacg ggttcccttt ttcgggatgg agtttcactc
     1681 cctctcccta tttttttccc gatctgattc tttattttcc tgtccgcctt ctttcaggaa
     1741 gctttcaggc atccatgtat gtttacggta tggtgcagat attcctgctc ttcagtcttt
     1801 cagtcttcat tgtccgttcc atgtttacaa agactgtatt tctgttttcc gcagcttcgg
     1861 gatatgttct gatcagtgta tgcataatac tgatatctga attcaggcct tttgccatta
     1921 ttctgattcc cactgtgcat ttcggggcct atctcatcct gctcgcctgt atcctgtact
     1981 ggcttaggaa tttcagtgcg ggatctgtaa ttcctgttct cacagtatgt gcaggtgttt
     2041 ccgacaggct ttttcatgtg atattttcta tcccttttgc attatcttgt atatttctct
     2101 ggagatatga ttcttcttct gaaacggaaa aatcaaaacc ttccttcatc agagagtttc
     2161 aggcatggaa gcctgcagtc aggagcatac ttctgtctgt cctcatttcc gttcttgtct
     2221 acaatggact gaaaagcctc atcaagctgg aggcagtttc aagggtcagc ccggatattt
     2281 cctggaaact ctttgttcag agcattctgt ctctgtattc caagggcggc atttctgcca
     2341 agattctttt tgcaggattt atacctgctt atactttctg tttcatatat tatattaaaa
     2401 aaaaagaata tcttcagcgg actgtccggt tttttcttct tttttctctg atatcagtgc
     2461 ctgtttcgct gtctgcaccg gtcgcggcgg gaacatttct ggatgaatac agttttaggt
     2521 attctgtatt cagttttttt cttctctact gcagtttttt cctggttctg tatgttcaat
     2581 tcagatttta cctgcgcaga acgaaacgca gagttctgga aacgcttgcc cgtatttcag
     2641 cgtatcagtt tatttttttc tcaatcctga tggaaggaat ccttattttt atgctggact
     2701 gggagagaaa aatgagagat aacttttcct atgttccgga tgaagtaaaa tgtgcagaga
     2761 gtattttggg gaataagatt gccgtatatg gactttcaga ctactggaat gcaaaatact
     2821 tcacactgtt ttctgaaaac cgtctcaggg tttatcagat cagttatcag gatctcagaa
     2881 aaaatcatac actgaacaat aaatactggt atgagaaagg aaaaagctcc cgattcggga
     2941 accctgaata taaattcatt atcactgaaa gactttcgga agttctcatt gagaaaaaat
     3001 ggggcagacc ggattcagtt catttatgtt cgggaaagaa tgtctggata tacagaaaag
     3061 gcatcagtct agattagaaa tttatttctg agatcgtcca ctttcttctt cagattctca
     3121 tccctttcca ttcctgattc cagttttttt atggcggcaa tcactgtagt atgctgtgtc
     3181 ttgaagagtt ttccgactga agacttgctc atttcacaga gttcatgaat gagatacata
     3241 ctgatatgtc tggggagtat aaactctgat cttctgcttt tgctcagtat gtctgatttt
     3301 tcgcgttcaa aatatgctga aacctctgtg agtattctgt ccggactcaa gtctcctgcc
     3361 ctggatttag tgattctgga ctcaaggatt tcgcgaattt tatcttcatt cagaatcagc
     3421 agattataag ttcttttata taatgaaagt tcattgaccg caccaagcag ggctcttgta
     3481 tcagaaggaa acagaaaaga aatcagatca acagattctt ctgaaagact gattccgcat
     3541 tctcttgatt taagactgat gatctttttt cggagtgatg aatccgcctg cttgacttcc
     3601 gcctgaatgc ctgtaagcag tctggaacga agtccttcct gaagaggaag ctccatcacc
     3661 ggtctgtctg cggccagcac tatctgcctt tttctttcct gcatataatt gaaaagagaa
     3721 taaaactctt cctgtgtttt ttctgcagtg atgttcagaa actgaagatc gtcaaagatg
     3781 atggaagtat aggactggta tttgtttcgg aatgattcaa gtgactgtct gttctgcacc
     3841 atcattacaa attctgaaag aaaggacgaa atggaaatgt acttcacatt ctcattcgga
     3901 tttttctgaa gctcctgacc gactgcatgc agaagatgag tcttgcctgt accgcatttc
     3961 ccataaagaa aaagaggatt gatttttcca ggagaactga tcacttcctt tgatgcaaag
     4021 taggccagct gattggattc tcctatctga aatgaattaa aagtaaagtc agggttgaaa
     4081 agaaatttct cttccttgaa tttctgcctg atgaattcac cgatcgattt tccctgttct
     4141 tcagtgaaaa tttccaccgc agaactgctt ccgtaaacct taaacactgc atcttcaatg
     4201 aacttctgat attttttttc aacatgttcc ttgactgcag aggagggagc cttgatgaaa
     4261 aattttccgt ctgaatattc ttcaaacagg agcggctcta taaaaggatg aaaatacact
     4321 tttggaatct gcttagaaac ttcttcaaga accaaattcc actcattttt catctttcct
     4381 ccgattgaga ttggtaaaat gcacgaacta cagatatact ataaaattcc tgcccccttt
     4441 atattaaaca aaaaactgac tgacttgaat tttttggagt tgaaaaaatc gaatgaacag
     4501 cttttaaaaa atttttaaaa aaaatcagag tggtacaaaa acaaaatgat acattagatt
     4561 attctaatat tctaggatgc cgcacacctg acggtttatc aataagttat ttttttaaag
     4621 gaaaaaactt aactgatttt gggcaattat aaattatgtt ctcataacag tcagtttttt
     4681 aaagccgatt tacagaatgt aaatgcatat tgctgaaaaa ccgctcaaaa ttcctttcag
     4741 ttatcaaccc tgttcttatt catcgctgaa cagtgttttt gttttatgct gatttattca
     4801 tctttgcata aaaattcagc tcagagaccg cggatctttt taaatttcag cagaagattt
     4861 tattgatttc tgatttgaga ttcagaagac tgactttggc cggaggaaaa gatgagcgag
     4921 tctcttgaaa atctttctca ttatatcagg aattcctttc attcctgtgt ccgaggaaga
     4981 cttattctac cggagattca gggagatttt gcagagtttc cttcttcaat gaatcctctt
     5041 ctcagcaagg cactgaatga tctgggaatt gaaaaactct actctcacca gaaggaaagt
     5101 tttgaatcag taagaaaagg aaacaataca attcttttat ccagaactgc cagcggtaaa
     5161 accttttctt ttctgcttcc tatcctcaat gattatgctg aaaaaccggg aagatttacg
     5221 actcttcttc tgtatccgac taaggcactt tcgagagatc aggaatcagc attggggaaa
     5281 attctgcgct cagtttccgg tgatgcaaaa ttcggagttt ttgacggaga taccgccaga
     5341 gaagacaggg aatcacttgt gaaatatgca gatttcatcc tgagcaatcc ggatatgctc
     5401 cattcaggaa tccttcccaa ccattccaga aaatggagaa actttctttc cagactcaaa
     5461 tacatcgtag tggatgagag ccatatctac agaggggcat tcggatcgca tgtttccaat
     5521 gtgatgagaa gactgatcag aatctgccgg aattacggaa cagatccgat attcatatgc
     5581 tccaccgcgt ccatcggaaa tcctgcggaa catgcaaaag cattatttca taaagatttt
     5641 aacttaattt ctaatgacgg atcaccgagg gcggaaaagg aaatactctt cctgaacccc
     5701 gcactcgtat ccagaaacgg aggaccggaa tacagacagg gaccggcttc catttccatt
     5761 ccggtcatct gtgaagccgc aagaatggga atccgcacaa tctgttttgc gagagccagg
     5821 caggaggtgg agagacttta caaggcagtg gcagagaagg attcttatat cagggaaaaa
     5881 gtaaaaccct acagaggcgg acttcttccc agtgaaagaa gagctctcga aaaggaactt
     5941 tttgcaggga atctgaatac aatcattact acaaatgcac ttgaacttgg aatagacata
     6001 ggagatatgt ctctctgcat tctgaacgga catcccggat cagtttcgag tttctggcag
     6061 caggcaggaa gagtcggaag ggcaggaaaa aaatcactca ttctgtatat tgcaaaagat
     6121 atgccgatcg atcagtacat tgtgcacaat aaggagttta tcatatccgc aggatcagag
     6181 gaagcctggc tttctgcgga caatccttat attattatgc agcatattcc ctgtgccgca
     6241 tatgagattc ctttttctgc agaggaaacg attttttccg gacctgtatt caatcttgct
     6301 ctttccaccc tcgaagaaaa aggaacactg aaaaaattcc gcgaatttta cagatacgct
     6361 gaagaggaat ttccctccaa gggagtgaat ctgagaggtc ttacagatta taatgtaaat
     6421 atattctggg acgggaaaat catcggtgag attgatccga tcggggcaat gggcactctt
     6481 tacaaggatg caatctatc
//
LOCUS       DAOQGJ010000013         6445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_5929_length_6445_cov_3.297809, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000013 DAOQGJ010000000
VERSION     DAOQGJ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5929_length_6445_cov_3.297809"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PK683_00405"
     CDS             complement(<1..881)
                     /locus_tag="PK683_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06931.1"
                     /translation="MKIKKIIIFNLCVLFQCLSLSSVFSQGGNLKKVFYASDSSRTGI
                     VKYSFSDSAPRRFEDLKEITLPEIKDLRLKFPSYTASAYIGPDGGDVYIWKKDQYQWK
                     RSDKSVFTEWPNGAWKLEQPEGTVINSFTKCAGCPPEAQIVFKDGSELLKNWVPHRKE
                     YDWIFQKDNTAPALNWKLVDPSKVQSGKAQLSKYTFYYSPEWDSFIAALKKRFPGDIF
                     FDYLKKEFGLENAGQIPVILFDNEKSLTAYAGRELPHGGSSGGGFGGRDSIIMDHYDA
                     EKPLTGDPVLDDYTLRMV"
     gene            complement(878..1708)
                     /locus_tag="PK683_00410"
     CDS             complement(878..1708)
                     /locus_tag="PK683_00410"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqG family protein"
                     /protein_id="HNH06932.1"
                     /translation="MKKFQLKKNEITEQKSSGQEVPEFAKFKIPSKFKNLDAKISSTL
                     KLSVSVELKGKPSHPWSSKIGGHPYLPVGTEYPSVKKVRPLFLLAQINFEEMPSLPDF
                     PEIGILQFFIGDNELYGLDLESPFKSDYKIVYYPEVIQDKKKLVSDFSFLPEFTYTPL
                     DAGFEAGMRFRRNAVSMPCNDYRFEDMFPELSDDYDLCDEYQEFCGALGTKIGGYPDF
                     TQEDPRVRKTLKGYELLFQLDSEGEVCWGDMGVGNFFIKPEDLKKKDFSKVLYNWDCT
                     "
     gene            complement(1671..2210)
                     /locus_tag="PK683_00415"
     CDS             complement(1671..2210)
                     /locus_tag="PK683_00415"
                     /inference="COORDINATES: protein motif:HMM:NF017243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HNH06933.1"
                     /translation="MRRRFEYRDEKSEKFWEIELKGSSFTTYYGKIGGGPRQDTKEWD
                     SPAEAKKEYEKIVAEKLKKGYEEVSEKGDAGKESKADSKKKKSIDADKKADKKPVNPG
                     KNRRRFEYIDEKSSKFWEIELKGTAYTTYFGKIGGSVRSDINDFGSEVDALKEYEKIV
                     AEKLKKGYEEVSVKKKRNN"
     gene            2495..3034
                     /locus_tag="PK683_00420"
     CDS             2495..3034
                     /locus_tag="PK683_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06934.1"
                     /translation="MMIYENFPTVEEILSEHRNQIGDVYAGYRNHVYRMIHFCLAFRE
                     CTETEKQKIFIAGAFHDIGIWTEDTLDYLPPSIELAAEYLKKKGLQDWEKEITLMIDM
                     HHKLTEYKSLDYPLVEVFRKADLADFSLGAVKNGLSESIVSEVKNAFPNSGFHKFLTK
                     LALKWAMKHPLNPAPIMKW"
     gene            complement(3095..4810)
                     /locus_tag="PK683_00425"
     CDS             complement(3095..4810)
                     /locus_tag="PK683_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06935.1"
                     /translation="MSKKEEIKGEVILDLNEELNDSELNDKNTEELAELIRDYWESYL
                     KMDKDSISEYLSDNVRKMSQRNRKLLDGSEAVLEDLPDEWTAFERPDHLISEKMTICH
                     INIWADDESSPKYAIVSYWVDIEGGVRWYYTDQGLVLQVFKKVDGDWKLVLQTDGWAT
                     DYDWDEKAPGEEPTFIFDYAYPVKDLDRAVKFYTPILGEPDYVTDTQAYFGLKDPGFI
                     LDSSGLFGYSEIKKDLCNGYGVIYVTNLEKEIEKMKEAEVEFLEDTDSEPKKWKEDLF
                     VIIKDAAGNITLVLERKYSSDEGDSEVKGFDEDSEYVEAAKAVAEAWMGKDTETISSY
                     VGKKGFWFDNSKLTNRGVHEGSEELTENLKKQYWKNLDYTEDGIIGIWEAENIKEISL
                     GDYTIVTYERSLEGSGNHPVKVSSFVTHIFESPKKLKFTFINNSTVKDSMALEVDYTA
                     NPVLSIKDAEEFYTEQLKLGELYDDEGWLGYWSNNAVYGVYKVDKGDKLLHDEKSNGY
                     VSFWIRSAEETNKYLKKMDVKFPLIESINTKKGVDAQHGYVQVVCSDSEGNIVLFSEY
                     TGRRK"
     gene            5294..5920
                     /locus_tag="PK683_00430"
     CDS             5294..5920
                     /locus_tag="PK683_00430"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH06936.1"
                     /translation="MKSQAFLVLDKTDGNQNHLSMAALILENSEVRKLVSVLSVEAYH
                     RLSELGLISSKTELIEGVVFDKMTKSPDYGYYSDLLFEFLSAIKPQDCIIRCEKPLTL
                     NRSEPEPDISIIKGSLQDFKNVNPSTAVLIVEIANTSISYDREKISVYAEAGVANYWI
                     ADLKKMQVETYQKPLKNEYLQKTVYGINDQIPIFDSFIDLQKVFTEKE"
     gene            complement(5924..>6445)
                     /locus_tag="PK683_00435"
     CDS             complement(5924..>6445)
                     /locus_tag="PK683_00435"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH06937.1"
                     /translation="REEYMHLPDDGFRYDMIDGVLHLAPSPFSAHSDYAGNFSGLLWN
                     YFRIKPIGKFFPELDVYLPDGGDTVRPDITAVLNENKKIIGKWIFGTPDLVCEVLSES
                     TRAYDLGKKADRYLSNGVKEFWIIDSDAKTVQVWKNRKTEWKKETASELFSEVLNGFS
                     LNCNDFFGTEQVG"
BASE COUNT         1810 a         1461 c         1140 g         2034 t
ORIGIN      
        1 accattctca gtgtataatc atccagtaca ggatctccgg taagaggctt ttctgcatca
       61 taatgatcca ttataatgga atctctgcct ccgaagcctc cgcctgaact gcctccgtga
      121 ggaagttctc ttccggcata cgcagtaagg cttttttcat tatcaaataa tataaccggt
      181 atctggcctg cattttcgag tccaaactcc tttttcagat aatcaaagaa gatatcgccg
      241 ggaaaacgtt ttttcagagc cgcaataaac gaatcccatt ccggagaata gtaaaatgta
      301 tacttggaaa gctgagcctt tcctgactga accttcgaag gatcgaccag cttccagttt
      361 aaggcaggag cagtattgtc cttttgaaaa atccagtcat attctttcct gtgcggaacc
      421 cagttcttca aaagctcaga tccatcctta aatacaatct gtgcttccgg aggacatcct
      481 gcgcatttgg taaatgaatt gatcactgtg ccttccggct gttcgagttt ccaggctccg
      541 ttcggccatt cagtgaatac agatttatca ctcctcttcc actggtactg atccttcttc
      601 cagatataca catctccccc gtctgggccg atgtacgcag aagcggtata ggaaggaaat
      661 ttcagacgga gatccttgat ctcaggaaga gttatctctt tgagatcctc aaaacgtctg
      721 ggagcagaat cggaaaaact gtattttaca attccggttc tgctggagtc tgatgcataa
      781 aaaactttct tcagatttcc tccttgagaa aaaacagagg agagagacag acactgaaac
      841 agaacacata gattaaatat aataatcttt tttattttca tgtgcagtcc cagttataaa
      901 gaaccttgct gaaatccttt tttttcagat cttcaggttt gatgaagaaa ttgccgaccc
      961 ccatatctcc ccagcagact tctccttcag aatccagctg gaaaagaagt tcgtatccct
     1021 tcagtgtttt tctcactctg ggatcttcct gagtaaagtc aggataccct cctatcttgg
     1081 ttccaagagc tccgcagaat tcctgatatt catcacagag atcatagtcg tccgaaagtt
     1141 caggaaacat atcctcgaac ctgtaatcat tgcagggcat tgaaacggca tttctgcgga
     1201 aacgcattcc agcttcaaat ccggcatcaa gaggagtgta tgtaaattcc ggcaggaaag
     1261 aaaaatcgga taccagtttc tttttgtcct gaatcacttc aggataatat acgattttat
     1321 aatctgactt gaaaggactt tccagatcga gaccgtacag ttcattgtcc cctataaaaa
     1381 actgaagaat tcctatttca ggaaaatcag gcagtgatgg catttcctca aaattgattt
     1441 gagcaaggag aaaaagaggc cttacttttt taacagaagg atactctgtt ccgacaggca
     1501 gataagggtg tcctccgatc ttcgagctcc aaggatgtga gggttttcct ttcagttcaa
     1561 cgctgactga aagtttcagt gtggaactga ttttagcgtc caagttttta aactttgaag
     1621 gaattttgaa ttttgcaaat tcaggaactt cctgtcctga agatttttgt tcagttattt
     1681 cgtttttttt taactgaaac ttcttcatag ccctttttca gtttttctgc aacgatcttc
     1741 tcatactctt tcaaagcatc cacttcagaa ccgaaatcat tgatatcaga tctgacgctg
     1801 cctccaattt ttccaaaata ggttgtataa gcagtacctt taagttcaat ctcccagaat
     1861 ttgctcgact tttcatcgat atactcaaac cttctccggt tctttcccgg atttactggt
     1921 ttcttatctg cttttttatc cgcatctatt gatttttttt tctttgaatc tgccttgctc
     1981 tcttttcctg catctccctt ttccgaaacc tcttcataac ctttcttcag tttctcagca
     2041 acaatctttt catactcttt cttggcttct gcaggtgagt cccactcctt tgtatcctgt
     2101 cttggaccgc cgccgatttt tccatagtat gtagtaaaag aacttccctt cagttcaatt
     2161 tcccagaact tttctgattt ctcgtctctg tattcaaacc ttcttctcat aaactctctc
     2221 atcaataaat atgataggcc actatccaca gttctcaaac tttgtaaaat aaattttttg
     2281 ctccttgccc tgttatactc atattcaaaa gtctttgcgt atttctactt tagtgagcct
     2341 gtaaaatcaa ggttttcgaa tgtaaaaagc gcaattactt ttggtaatca gtataactct
     2401 ctgcgaaggc aagtttatca cacaatttct gagtcttttc tctgccgtac cagctatcag
     2461 ttgacaaaag cagattcact gctttcatag aacaatgatg atttacgaaa actttccgac
     2521 tgtagaggag attctttcag aacacagaaa ccaaatcggc gatgtctatg ccggatacag
     2581 aaatcatgtt taccgaatga ttcatttctg tcttgctttt cgggaatgca ctgaaacaga
     2641 aaagcagaag attttcatcg caggtgcatt tcatgatatc ggaatctgga cagaagatac
     2701 attggactat cttcctcctt cgattgaact tgcagcagaa tatctgaaaa agaaagggct
     2761 tcaggactgg gaaaaggaaa tcactctgat gatagacatg catcacaaac tcactgaata
     2821 taaaagttta gattatccac ttgtagaagt attcaggaaa gcggatcttg cagacttttc
     2881 actgggtgca gtaaagaatg ggctgagtga aagcattgta tcagaagtga aaaacgcatt
     2941 tccaaattca ggatttcata aatttctcac aaaacttgca ttgaaatggg caatgaaaca
     3001 tcctttaaat ccggcaccta ttatgaaatg gtaactcctg cagaaggcag tcctaaacct
     3061 gacgactgcc tttaatcaga ggattttgaa tgctctattt tcgtcttcct gtatattcac
     3121 tgaagagaac aatatttccc tcactgtctg aacaaaccac ctgaacgtag ccatgctgag
     3181 catccacgcc tttctttgta ttaatggact caatgagagg gaacttgaca tccattttct
     3241 tcaggtactt atttgtttct tcagcggacc gtatccagaa actcacatat ccgtttgatt
     3301 tttcgtcatg aagaagctta tctcctttgt caaccttata tactccatat accgcattat
     3361 tgctccagta tcccagccat ccttcatcat cataaagctc acccagtttc agctgttctg
     3421 tataaaattc ttctgcatct ttaatgctca ggacaggatt ggcagtataa tccacttcca
     3481 aagccataga atcttttact gtagaattat ttataaatgt aaatttcagt tttttgggag
     3541 attcaaaaat atgagtcaca aatgaactta ctttcactgg gtgatttccg gaaccctcaa
     3601 gagacctttc ataggtcaca attgtatagt cgccgagaga tatttccttg atattctccg
     3661 cttcccatat gcctatgatt ccgtcttcag tataatccag atttttccaa tactgctttt
     3721 tcagattctc agtcagttct tcacttccct catgaactcc tctgttagtc agcttactgt
     3781 tgtcaaacca gaaccctttc ttgcctacat aggaggagat tgtttcagta tcctttccca
     3841 tccatgcttc tgcaactgcc ttggcggctt ctacatactc actgtcttca tcaaatcctt
     3901 ttacttctga atcaccttcg tcagaactgt actttctttc cagaaccagg gtgatatttc
     3961 cagctgcatc ttttataatt acaaacagat cttctttcca ttttttgggc tcggaatccg
     4021 tatcctccag aaactcaact tctgcttctt tcattttttc aatttctttt tcaagattcg
     4081 taacataaat gacaccatat ccgttgcaga ggtccttttt gatctcggaa tatccgaaaa
     4141 gtccggaact gtccagaata aagcctggat ccttcagtcc aaaataggct tgagtatcag
     4201 ttacataatc cggttccccg agtatcggag tgtaaaactt gacagcacgg tcaagatcct
     4261 ttacaggata agcgtagtcg aatatgaaag tcggttcttc accgggagct ttctcatccc
     4321 agtcatagtc cgtcgcccag ccgtctgtct gcagaacgag tttccagtcc ccgtccactt
     4381 ttttgaatac ctgcagaacc agaccctgat ccgtgtagta ccagcgaacc cctccttcaa
     4441 tgtccaccca gtaggaaacg attgcatact tgggagaaga ttcatcatca gcccaaatat
     4501 taatatgaca tattgtcatt ttttcagata ttaaatgatc cggtctttca aaggcagtcc
     4561 attcatccgg cagatcctcc agaacagcct cagatccgtc gagcagcttt ctgtttctct
     4621 ggctcatctt tcggacatta tcggagagat attcagaaat actgtcctta tccatcttca
     4681 gataactttc ccagtaatct ctgatcagtt ctgccagctc ctctgtattt ttatcattta
     4741 attcagaatc attcagctct tcattcaaat cgagaatgac ttctcccttg atttcttctt
     4801 ttttggacat agacggatct ccctgtgatg aatatctacg cagagcattg gattccttca
     4861 ccggtttttt tcaaggactt ttataatatt gcggatattg actttttatc agtttgcaat
     4921 gactgactca gcagggatgt tttccccggc agattcacac tttctcagca gaccttccat
     4981 actgttctct ttggatttga ccgatgtgaa ttcgcaactg aggcaataaa taaattataa
     5041 agtttattat cattattttc actcataaaa ttaaaggaac acagttgaaa gatccagctt
     5101 aatctgcgaa gaaaaaagcg gcgatagaat ttcattcctg gaataggtct tcatttcaga
     5161 atatctctca cctgaaggct ttaagctgac ttccacagta ctgttgtaga gattgaacag
     5221 ccaatattca ggaaggcctg cttcagcata aatacaggct atttctctgt cttctgcaag
     5281 aaatgcagat ctgatgaaaa gtcaagcttt tttggtgctt gacaaaactg atggaaatca
     5341 gaaccatttg agtatggccg ctttgattct ggaaaattcg gaagtccgca aacttgtttc
     5401 ggttctttca gtagaggcct accatcgtct gagcgaactg ggtttgattt ccagcaaaac
     5461 agaactgatt gaaggagtgg tattcgacaa gatgacgaaa agccctgatt atggttatta
     5521 ttcagatctg ctgtttgaat tcctttcggc cataaaaccc caagactgca ttatccgatg
     5581 cgagaagccg ctcacattaa atagatcaga accggaaccg gatatttcca tcataaaggg
     5641 ttctcttcag gactttaaga acgtcaatcc atccacagct gttttaatcg tggaaattgc
     5701 gaacacatcc atcagttatg atcgggaaaa aatctccgtt tatgcggaag ccggtgtggc
     5761 aaattactgg attgctgatc tgaaaaagat gcaggtagaa acttatcaga aaccgttaaa
     5821 gaatgagtat ctgcaaaaaa ctgtatatgg cataaatgat cagattccga tctttgattc
     5881 atttattgat cttcagaaag tgtttactga aaaagaatga aattcagccg acttgctcgg
     5941 ttccgaaaaa atcattacaa tttaatgaaa atccattcag aacttcactg aacagttccg
     6001 aagcagtttc ttttttccac tcagtcttcc tgtttttcca gacctgaacg gtttttgcat
     6061 ctgaatcaat aatccagaat tctttgactc cgttagacag atatctgtct gctttttttc
     6121 ccagatcata agcacgcgta gattcactca gcacctcgca gaccaagtca ggtgttccga
     6181 aaatccactt ccctataatt tttttattct cgttcagaac tgcggtaata tccggcctga
     6241 ctgtgtcacc gccgtcagga agataaacat ccagttcagg aaaaaatttc cctattggtt
     6301 ttatccgaaa atagttccaa agcagaccgg aaaaatttcc ggcatagtca gaatgtgcag
     6361 aaaaaggact tggtgccaaa tgcaatactc catcaatcat atcataacga aacccgtcat
     6421 ccggaagatg catatattct tccct
//
LOCUS       DAOQGJ010000014         6358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_6057_length_6358_cov_3.635253, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000014 DAOQGJ010000000
VERSION     DAOQGJ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6057_length_6358_cov_3.635253"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK683_00440"
     CDS             <1..227
                     /locus_tag="PK683_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06938.1"
                     /translation="ESKMKNYSGKIQRLLENHFNLKEEYQKVYNHSYGSGMDTALKAL
                     GIPLEGTHHRGIDDAVNIAKIFKAIFHSLT"
     gene            complement(220..1722)
                     /locus_tag="PK683_00445"
     CDS             complement(220..1722)
                     /locus_tag="PK683_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH06939.1"
                     /translation="MRKFFSQLELLGFFKKTFKKLKERDFSFSWSEFRTRFQMLPLYH
                     DFVQFVLSITSILRLISHTSERVTDNTVKMASLSERLKNDSDNIREIMDSLVLEMKSL
                     TDEVKNISSEVSEIRRQSNHMLQVNRNILESSQSIQRRVNVGVSDMQNGVFLISELVE
                     QNKELSGTIHDLWSKYSYLASYARDLIKLSENTKMLALNAEIEAAHAGEAGKGFAVVA
                     QEMGNLSHKNSEISRMIFRGIIGMQDQAKLTDINVASSVELAENSEEEIGKVHKSYED
                     VKESIEAVLHDTEDFTESFASLEISLKLINEHINQTQSHLLATSGDAKKISDSIDSQQ
                     RSVEQMNSEISSAFHASRILNSMIAQFKIPNFNLISQKRSLIEFLLQGIMNIRGIYVI
                     VFFSDSASLPTYIFDERKIIEADVRKNIAQLETLLYQKDEIETFKSFLFNWENYWKKS
                     ENCLQLVTEGNARTAREKYESEIKELLSPAVNSLLELILFDLNETVQKAM"
     gene            2163..2552
                     /locus_tag="PK683_00450"
     CDS             2163..2552
                     /locus_tag="PK683_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNH06940.1"
                     /translation="MNKVSKTAAAVLTGLNAFIHSYILVLEMFLWNTPYGQKAFNMTA
                     EKAEITSVLAANQGLYNGFLAAGLIWSFFISDLKMKKQVRIFFLACICTAGIYGAATA
                     AVKILFIQSVPSALAILAILALKDENI"
     gene            2613..3452
                     /locus_tag="PK683_00455"
     CDS             2613..3452
                     /locus_tag="PK683_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH06941.1"
                     /translation="MQADPMNEEHSLIDSALSGNSKALENLIAGVQKKIFNLSLRFLW
                     DPMDAEDASQEILIKAVTHLDSFQKQSSFATWVHRIAVNHLIEFKRKTAKLSRINFAI
                     AGRELKKQEAPPAEMDEDEITETAAKVKTACTFGMLQCLKPESRMAFVLGTVFETDSE
                     EGSEILGISASAFRQRVSRARRKLEDFIFSRCSVVNHSAPCRCTGRITPGLRNSTLQP
                     YLNLAEQLEEKGKNSELFFYEKEIRRTEKVMALYKRASQYPAPANIQNRIKKLFSDST
                     LYK"
     gene            complement(3499..4467)
                     /locus_tag="PK683_00460"
     CDS             complement(3499..4467)
                     /locus_tag="PK683_00460"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH06942.1"
                     /translation="MERRKHLIRSIVQKWLNRQTRLPIILVQLRDIILIRFGVISALS
                     LFFGLIFFIVLFKELGIQDKVIRILLIFGPISLIALSFVLSWIISIRKILEDIRNLRN
                     VSFGFFGGFLGVVLTFIIVSLKYDINLLLVSDGGAVLTGFIHSFARTACLNYGCCHGK
                     EIQGELNGRLHLVYTDPLAKAVRISGLEGRPLYPIQFYESISCLGLGFLTVLLCSFHP
                     PAGTLTGFYFLGYGIIRFICEFYRGEADTASYRNLTVYQWISLGLVLIGSSVTVYSFL
                     FGKPASIVFHLGTFTLIGDFLPFIIGMPFIIFFAYGYHYKTIGRWC"
     gene            complement(4446..4928)
                     /locus_tag="PK683_00465"
     CDS             complement(4446..4928)
                     /locus_tag="PK683_00465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH06943.1"
                     /translation="MNLSLIMANENQYKLKTVYVGTRGVQEYRDSIGTYVSSSDRVLE
                     IGCEWGTTSAHIFPKCEKLIATDISPKCIEKAKEIRPEIHFETLDVYDIRRAQSFGVE
                     FNKMYIDVSGLSGYRSVLDVLALLNMYASVFPMDAIVVKSGALKDLSRRCIAWNGGNI
                     "
     gene            5141..6106
                     /locus_tag="PK683_00470"
     CDS             5141..6106
                     /locus_tag="PK683_00470"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH06944.1"
                     /translation="MDDFPPLKTGAEVPELSDIEWLKGSSRKLGETLTLVEFWATWCG
                     PCLQSIPHLTELQKKYKGKLTVIGLSNEEKAIAAPFVKQQGSNMDYTVGIASEELYSK
                     YMAGVEGIPHSLLINKNKKLIWSGHPLDIDNTLTSLLEKGVSEEEIMLPMKEQEEFNE
                     TLNWIIQAGLQDNWEKRKEELYGIGAKILKRNPTDFRVMQSLTYVAANYCGLEEMNKV
                     CDSFNVSSYSAEDFRILVEQFVLIIPVDFRPYSNAVKWIKTGLSKFPKNADILYAYSK
                     LLADIGFFDEAVRAAQEGLSLNPDMEELQSYMEVLKRIKIARTFI"
BASE COUNT         1998 a         1334 c         1267 g         1759 t
ORIGIN      
        1 gggaatcaaa aatgaaaaac tacagcggta aaattcagag acttttggaa aatcatttta
       61 atctgaagga agaatatcag aaagtataca atcattctta cggatcagga atggacactg
      121 cattgaaagc acttgggatt cctctggaag gaactcatca caggggaatc gatgatgcag
      181 tgaatattgc taagatattc aaggcgatat ttcacagcct tacatagctt tttgcaccgt
      241 ttcattcaga tcaaaaagta tgagctcaag aaggctgttt acagccggac tgagcagttc
      301 cttgatttca gattcatact tttctctggc tgttcttgca tttccttctg taaccagctg
      361 aaggcagttt tctgattttt tccagtaatt ttcccagttg aataaaaagg acttgaatgt
      421 ctctatttca tccttctgat aaagaagcgt ttccagctga gctatgtttt ttctgacgtc
      481 ggcctcgatg attttccttt catcgaagat ataggtaggg agagaggctg aatcagaaaa
      541 gaatacaatg acatatatcc cgcgtatatt cataataccc tgaagaagga attcaatcaa
      601 tgatctcttc tgggaaatca gattgaaatt tggaatctta aactgtgcga tcattgagtt
      661 taagatccgg ctcgcatgaa atgcggatga tatctcagag ttcatctgtt ccactgatct
      721 ctgctgagaa tcaattgagt cggaaatctt ttttgcatct ccgctcgttg ccagaagatg
      781 agactgcgtc tggttgatat gctcattgat cagcttcagg ctgatttcca gacttgcaaa
      841 cgactcagtg aaatcttcag tatcgtgcag caccgcctca attgattctt ttacgtcctc
      901 ataactcttg tgcacctttc cgatttcttc ctctgaattt tcagccagtt caacagagga
      961 ggctacattg atatcggtaa gctttgcctg atcctgcatc ccgataattc ccctgaatat
     1021 cattctggaa atttcactgt tcttatgaga aagatttccc atctcctgag cgacaacggc
     1081 aaaacccttt ccggcctcgc ctgcatgagc tgcttcaatc tccgcattaa gtgcaagcat
     1141 cttagtgttt tctgaaagct taatcagatc ccttgcataa ctcgccagat aagaatactt
     1201 tgaccaaaga tcatgaattg taccgctgag ttccttgttc tgttctacaa gttcagaaat
     1261 aagaaaaaca ccgttctgca tatcagaaac gccgacattc actcttctct gaatgctctg
     1321 cgaagattca aggatattcc tgttcacctg aagcatatga ttgctctgcc gtctgatctc
     1381 ggaaacttcg gatgaaatat tctttacttc gtccgtcaga cttttcattt ccagcacaag
     1441 agaatccata atttccctta tattgtctga atcgttctta agtcgctcgg aaagagaagc
     1501 cattttcaca gtattgtcag tcacacgttc agaggtatgg gaaatcagtc tcaggatgga
     1561 agtaatgctc agtacaaact gaacaaaatc gtgatagaga ggaagcatct gaaaccttgt
     1621 acgaaattcg ctccaactga atgaaaaatc acgttctttc agttttttaa aggttttttt
     1681 aaaaaaaccc agcagttcca gctgactgaa aaattttctc aatgaattac ctgccagtca
     1741 gtcagtgtcc cgatcaatat ccattccgag atgaaaaaaa ctttcaaaat acgcaatata
     1801 cggcctttcc cctattgata ttcgttcaaa acaggtttcg tgattatgaa tgaaagttta
     1861 ttaaaaataa attcgtattc tgtcaaatac tttactatgt tttctgatta taccgggata
     1921 atatccgaaa atccattccg ctgagggaat atcactgtct ttgctgaaaa aacctgtcca
     1981 gaaggcgaat tcagaaaaaa aaagtgaaaa atggtcacag aatctgtaca gcctcatcta
     2041 atttttcagt aaaacaaagg agacgtttca aaaatatttt gacaaagcag aatactttcc
     2101 tactttcgta gaacgtttct gagtaaaaaa tatttggaac agctctttta caggagaaaa
     2161 gtatgaataa agtatcaaaa acagcggccg ctgtcctcac aggtttaaac gccttcattc
     2221 attcctatat tttagttctg gaaatgttcc tctggaacac gccttacggc cagaaagctt
     2281 tcaatatgac cgcagaaaaa gcggagataa cctcagttct tgccgcaaat cagggattgt
     2341 acaacggatt tcttgcagca ggactcatct ggtctttttt tatttcggat ctgaaaatga
     2401 aaaaacaggt caggattttc ttccttgcct gcatatgcac tgccggaatt tatggggccg
     2461 cgacagcggc agtcaaaatc cttttcatac agtcggttcc atcagcactg gcaatccttg
     2521 cgatcctcgc attaaaagat gagaatatct gagttttccg gacagtttgc tgataatttt
     2581 cggacaaggg aatctcagca gtctgtccag taatgcaggc tgatcctatg aatgaagaac
     2641 acagtctgat cgattcggca ctttccggga actcgaaggc tttggaaaac ctgatcgcag
     2701 gagttcagaa gaaaattttc aatctctctc tgcgttttct ctgggatccg atggatgcgg
     2761 aagatgcatc acaggaaata ctgatcaagg cagtcactca tctggacagc tttcagaaac
     2821 agagttcatt tgccacatgg gtacacagaa ttgcagtcaa tcatctgatt gaattcaaaa
     2881 gaaaaaccgc aaagctcagc agaattaatt tcgcgatagc aggcagagaa ctgaaaaaac
     2941 aggaagctcc tcctgcagaa atggatgagg atgaaattac agaaacagcg gcgaaagtaa
     3001 agactgcctg cactttcgga atgctccaat gtctgaaacc ggaatcaaga atggcttttg
     3061 tgcttggaac cgtatttgaa acagactcag aagaaggatc ggaaatttta ggaatttctg
     3121 cttctgcttt ccggcagaga gtttccagag cgagaaggaa actagaagat tttattttct
     3181 ccagatgcag cgttgtaaat cattctgctc cctgcagatg tactggaaga ataacaccgg
     3241 gactccgaaa cagcacactg cagccttatc tgaatcttgc agaacagctg gaagaaaaag
     3301 gaaagaattc ggaattgttt ttttatgaga aggaaatacg caggacggaa aaagtgatgg
     3361 ctctttacaa aagagcgtca cagtatcctg ctccggcaaa tattcagaac aggataaaaa
     3421 aacttttttc tgattcaaca ttatacaagt gactctgctg gaaaagtaaa tgccaggcaa
     3481 tccccttcat tcagcagatc agcaccatct tccaatggtt ttataatgat aaccgtaagc
     3541 aaaaaatata ataaaaggca taccgattat gaaaggcaga aagtccccga tcagagtaaa
     3601 agtacctaga tgaaatacaa tcgaagcagg ctttccgaat aaaaatgaat aaacagtgac
     3661 ggaactcccg atcagtacga gacccaagct gatccactga tatacagtca ggttcctgta
     3721 agatgccgta tctgcctctc ccctgtaaaa ttcgcatata aatctaatga ttccgtatcc
     3781 cagaaaataa aatccagtca gtgtgcctgc cggaggatga aatgagcata acagaacagt
     3841 cagaaatcca agaccaagac agctgatgct ctcataaaac tgaattggat acagaggtct
     3901 tccttcaagt cctgagatcc gaaccgcctt tgccagggga tcagtgtata caagatgaag
     3961 cctgccgttc aattcacctt gaatttcctt tccgtgacag catccgtaat tcagacatgc
     4021 agtccttgca aaactgtgga tgaaacccgt cagaacagct ccgccgtcag aaaccagaag
     4081 aaggtttata tcatatttta atgatactat aatgaatgtc aggacgaccc ccagaaatcc
     4141 tccgaaaaat ccgaaactga cattcctcag atttctgatg tcctccagaa tttttctgat
     4201 ggaaatgatc cagctcagaa cgaatgaaag tgctatcaga ctgattggtc cgaagataag
     4261 aagaattcga atcactttgt cctggatccc aagctccttg aaaagcacaa taaaaaatat
     4321 cagtccaaaa aaaagcgaaa gagcactgat gactccgaag cggatcagta taatatctct
     4381 gagctgaaca agaatgattg gaagcctggt ctgtctgttc agccattttt gaacaatgct
     4441 ccgaatcaga tgtttcctcc gttccatgca atgcatcttc tgctgagatc cttcaaggct
     4501 ccgctcttca ctacaatcgc atccatcgga aatacagatg catacatatt cagaagtgcg
     4561 agaacatcca gcacggaacg gtaaccggaa agcccggaaa catcgatgta catcttgttg
     4621 aattccacac cgaatgactg agcccgtctg atatcataaa catccagagt ttcaaaatga
     4681 atttcaggtc tgatttcttt cgctttttct atgcatttcg ggctgatgtc agtcgcaatg
     4741 agtttttcac atttaggaaa tatatgtgct gaggtagtgc cccattcaca tccgatttca
     4801 agaactctgt ctgatgaaga aacatatgtt ccaatagaat ctctgtattc ctgaacacct
     4861 ctggtcccca cataaacagt cttaagttta tactgatttt cattcgccat aatcaatgac
     4921 agattcaaaa acactcattt atttgcaaaa gaaaaaatgg agaatttact gatgagatgc
     4981 ttgaacatcc attcaacagt ttgaaccgcg cgaaaaactt ttttcttgct ttttcctagt
     5041 atgaatatct atatgattaa gaattgcatc tgcattgcaa acagggaaac tccctttcct
     5101 gcagaaggca aattcaataa acggatacag cggtaatgat atggatgatt ttcccccttt
     5161 aaaaacaggc gcagaagttc ctgagctttc agatattgag tggctgaaag gaagcagcag
     5221 aaaactcgga gaaacactga ctttagtgga attctgggca acatggtgcg gcccctgcct
     5281 tcagtccatt cctcatctga cagaacttca gaaaaaatac aagggaaagc ttactgtcat
     5341 cggactgtcc aatgaagaaa aggcaattgc cgctcctttt gtaaaacagc agggaagcaa
     5401 tatggattat acagtcggta ttgcatctga agaactttat tctaagtata tggcaggcgt
     5461 agaagggatt cctcattccc ttctcatcaa taaaaataaa aaactgatct ggtcaggaca
     5521 tcctttggac attgataata cactcacttc tcttcttgaa aaaggtgtat cggaagagga
     5581 gattatgctt ccaatgaaag aacaggaaga attcaatgag actctgaatt ggattataca
     5641 ggccggtctg caggacaatt gggagaaacg caaggaagag ctctacggaa taggcgcaaa
     5701 gattctgaaa agaaatccga cagatttcag ggtaatgcag tctctcacat atgtagcggc
     5761 caactactgc ggattagagg aaatgaacaa ggtctgtgac agttttaatg tttcctcata
     5821 cagcgcggaa gatttcagaa tcctggttga acagtttgtg cttatcattc ctgtcgattt
     5881 ccgtccgtac agcaatgcag taaaatggat aaagaccgga ttaagcaaat ttcctaaaaa
     5941 tgcggatatt ctgtatgctt attctaaact gcttgctgat attggctttt ttgatgaagc
     6001 tgtccgtgca gcccaggaag gactttcact caatcctgat atggaagaac tgcagtccta
     6061 tatggaagta ctgaaaagaa taaaaattgc gagaactttc atttaatctt aatatgaaat
     6121 acggtgcttt tgtttttcaa tacgacagca ggcttgctga tcctcaaaga actgtcatgg
     6181 tgagtttatc aaatccatgg cgttcgcacc cttcgacacg cagacgatga aactgccgca
     6241 aactaaaaaa ctggtgcaaa ctaggaaact gccgcaaacg atgaaactgc tgctcagggt
     6301 gacatttctc tttacttctg aaattatgac tttactgcaa tttgccgaca gatagatg
//
LOCUS       DAOQGJ010000015         6271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_6179_length_6271_cov_3.762870, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000015 DAOQGJ010000000
VERSION     DAOQGJ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6179_length_6271_cov_3.762870"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK683_00475"
     CDS             complement(<1..345)
                     /locus_tag="PK683_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNH06945.1"
                     /translation="MIATLPIFPLSGVLLFPGTYLPLHIFEPRYRLMMDYCLENDNEI
                     GITSYRDSNLIEPVFGWGKIIKRDPFPDGRSNIIVNGMGIAKLVRFKTVEPFIIASVE
                     KRPNSYQHLHTVE"
     gene            complement(388..1440)
                     /locus_tag="PK683_00480"
     CDS             complement(388..1440)
                     /locus_tag="PK683_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNH06946.1"
                     /translation="MDDNPRNIQVLGTVLSEKGYNIIIANDGIQTLKTVEKIIPDLIL
                     LDINMPTMDGFETCRKLKESELYSEIPVIFLTARTETEDIIKGFHYGAVDYITKPFNQ
                     AELLARVKTHLELRDKSEELKERNLKVQELLRVLLHDLSNPLSGIKGVIDFGQTNPEI
                     YSSANELMKRAVNNCFSIIDNVRKMRAVDEGKMEIQLQRFPLKECIQESLLLFDKRLQ
                     EKNLEVILDVPEGLNVLADKAIFLNSVFSNILTNAVKFSFPNSKIEVSAFSETEGRTV
                     LSVKDSGIGMPDWLLQDIFSPVKPTTRRGTSGEEGTGFGMPLVKKFIEYFQGTIEIFS
                     SEKEPKGTEVKIYLKS"
     gene            complement(1473..5582)
                     /locus_tag="PK683_00485"
     CDS             complement(1473..5582)
                     /locus_tag="PK683_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH06947.1"
                     /translation="MSYILNIYNKFRQRMKIPSPQAFSEEEFSYLILKASMSVMAVII
                     VVGEILMLFFGISERRQLILVLLSLFLSISYFLLRSNKIKAAKFLLVFGFWSVLITAT
                     LINGGIMAPAFSGFHILTAGSALLSLPRVSIAAAVFIPSYGFVLLKLMQNSLISYPYA
                     NDEAVLNFLVIQSMIGFGIAFITLSALKLNKAASEKAQREIDERENARKHLEELNRSL
                     ENLVRQSNEQLEEGRKTRERIEREKKESESDFKEFFENTSDLIQNVRVSDGRFIYVNK
                     AWLESLEYTADEIKKITIYDIVHPDSREKCMRILKELSSGKNIPFFESAFISKYGKKI
                     YIQGSGGCVFRDGKPYSARGILRDITQYKKAELALLDSTAKIKDFAAALDEYAIVAMT
                     DSKGNIIYANEQFCRMSKYSQEELIGSNHRIVKSGFHPPEFYAEMWETVLSKKVWKGE
                     VKNRAKDGSLYWVDTVIVPFLNAEGEIYQYVSIRKDITARKEMERALSESEEQYRSLV
                     ENAPDIIMKVDLNNRILFISRTAEGFTKEQVVGQNACDFVLPEYRDLVSQKHRESAET
                     GKPLSYETKFINSDGRSSWYLTHVSPIFSGGSVKELTLITRDISARKQLEEKLFEQKN
                     FIQKVTDSIPGLIGYWNREKKCEFANLAYREWFGRSSEEVLGMEMKDLLGADLYNLNL
                     PYVEKALAGEMQRFERKIVKPDGSAGITWAQYIPDIENNIVKGFIVLVTDITETKKTE
                     TSLQTAQNKLKEILSSIPEGIVEVNLQGEIVYANKEAGRILDIYESDIVGKYFNAENW
                     KQLDENGDPFPLNRLPLAAVLENQKADGPIEHMIENRHGERKWLSVHAVPLFNNDNIM
                     YGAAASFQDITERKNYQINLINAKEEADRANSAKTEFLANMSHEIRTPMNAILGFSEL
                     LNEKMEDQVLKQYAASIITSGRILLKLINDVLDLSKIESGKMDLNFSGTDVRKLLEEM
                     KTVFSQKISEKGLNFIIETDPSLPKSIMIDSMRLRQVLLNLTGNAVKFTDSGYIRLKA
                     ERLNPESLDRMFDLKICIEDTGQGIPVSDQEKIFTAFTQKTGQDHSKYGGTGLGLAIA
                     KKLTSLMDGEIALESQIGKGSAFTVILHNITSDTDFQDSLEDSPQISGEFSFSDSVIL
                     VVDDVFLNRELISQFLGKYSELKIIHAENGKEAVEKTLEFNPDFILMDIKMPVMDGIE
                     AIKEIRKYEEYASTPIAALTASVFEEERITREADIQGYMHKPVSRTDLLKLLSKYLGS
                     PESNDSSASSEKNELSEEEAVGSRELLSILEHEKIFQIHELLEVTVISEIRKFSEDIY
                     VLGMQYNYKPVIQWAENMRRHMSVFDVEKINSELSRFFEIIIELREACR"
     gene            complement(5613..>6271)
                     /locus_tag="PK683_00490"
     CDS             complement(5613..>6271)
                     /locus_tag="PK683_00490"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH06948.1"
                     /translation="KCSTAASFKDWSRKDKPVVYSGVRSCWSGIPGGQGPVAGPAALF
                     GALYCLIDTPFSSVLDTLLLPVSLPFSALKYLYYKDDIYIAVKENNAAEVRYCIRQGA
                     NLNSEDFSGRTVLDYAFEKCILHRQGSSENTPHKYAGNRQIAELLFAGGARKTSSKAG
                     NTALHFAAYCGYTDMAASLLQEGFRYDQKNKEGLSPMDIAEKENQKAVSDLFRKTVRQ
                     "
BASE COUNT         1640 a         1453 c         1155 g         2023 t
ORIGIN      
        1 ttctactgta tgaagatgct gataactgtt aggtcttttt tccacactgg caataatgaa
       61 cggttccacc gtcttaaatc ggacaagttt ggcgataccc attccgttta caatgatgtt
      121 ggatctgccg tcaggaaacg gatctctctt gatgattttc ccccatccaa atacaggttc
      181 aatcagattt gaatctctgt agcttgtaat tccgatttca ttatcatttt ccagacagta
      241 atccatcatc agtctgtatc tgggttcaaa aatatgcaga gggagatatg tgcccggaaa
      301 aaggagcacg ccactgagcg gaaatatggg cagtgtggct atcataaagc tatttaaaac
      361 attctgctga agctgtcaaa atgtttttta tgacttcaga tagattttta cctctgttcc
      421 tttaggttct ttttctgaag aaaaaatctc tattgtccct tgaaagtatt cgataaattt
      481 ctttactaaa ggcattccga aacctgtccc ttcttcgccg gaggttcctc ttctagtcgt
      541 aggcttgact ggactgaata tatcctgaag aagccagtca ggcattccta ttcctgaatc
      601 tttcacgctc agtacagttc ttccttctgt ttcagaaaac gcactgactt cgatctttga
      661 attcgggaaa gaaaacttga ctgcatttgt cagtatattg ctgaaaactg aattcagaaa
      721 aattgccttg tctgcaagca cattcagtcc ttctgggaca tccagaatca cttccagatt
      781 tttttcctga agtcttttgt caaagagcag aagagattcc tgaatgcatt ctttcagagg
      841 aaaacgctga agctggattt ccatctttcc ttcatcgact gccctcattt ttctcacatt
      901 gtctataatg gaaaaacagt tgttgactgc tctcttcatc agttcatttg cagaactgta
      961 tatttccgga tttgtctgtc caaagtcaat gacacctttg attccgctca gaggattgct
     1021 gagatcatgc agaagcacgc gaagcagttc ctgaaccttc agatttcttt ccttcagttc
     1081 ctcagacttg tcgcgaagtt cgagatgcgt cttgactctt gcgagaagct ctgcctgatt
     1141 aaacggtttt gtaatataat ccactgcacc gtaatgaaaa cccttgatga tatcctctgt
     1201 ttccgttctg gctgtaagaa atattacagg gatttcagaa taaagttcag actccttcag
     1261 ttttctgcat gtctcaaatc cgtccatagt aggcatattt atgtccagaa gaatcagatc
     1321 aggaataatt ttctcgacag tcttcagtgt ctgaatcccg tcgttcgcta taatgatatt
     1381 atatcccttt tccgaaagta cagttcccag aacctgaata tttcgtggat tatcgtccac
     1441 aataagaatc tgatgatttt tcctgtcaga gctcatctgc aggcttctct cagttctata
     1501 atgatttcaa aaaatctgct cagttctgaa tttatctttt cgacatcgaa tacagacata
     1561 tgtctgcgca tattttctgc ccactgaatg acaggtttat aattatactg catcccaaga
     1621 acataaatat cctctgaaaa tttcctgatt tcagaaatta cagtgacttc cagaagttca
     1681 tgaatttgaa atattttttc atgttccagt atgctcagaa gctctctgct tccgacggcc
     1741 tcttcttcac tcagttcatt tttttccgat gaggcggaag agtcattaga ttcaggactg
     1801 cccagatact tggacagaag tttaagcaga tctgttctgc tcacaggttt atgcatatac
     1861 ccctgtatgt ctgcttctct ggtaatccgt tcttcttcaa aaaccgatgc cgtaagggca
     1921 gcaataggag ttgaggcata ttcctcatat ttcctgattt ccttgattgc ttctattccg
     1981 tccataactg gcattttaat atccatcaga ataaaatcag gattaaattc cagtgttttc
     2041 tcgactgcct cttttccatt ttctgcatga atgatcttca gttcagagta tttcccaagg
     2101 aactgagaaa tgagctctct gttaagaaaa acatcgtcca ctaccagaat gactgaatca
     2161 gaaaagctga actccccgga tatctgcgga gaatcttcca gtgaatcctg aaagtctgta
     2221 tctgaagtga tattatgcaa tatgacagta aatgcagatc cttttccaat ctgactctcc
     2281 agcgcaattt ctccatccat cagactggtc agctttttag caattgccag tcctaatccg
     2341 gtccccccgt acttgctgtg atcctggcct gtcttctgcg taaatgcagt gaatattttt
     2401 tcctgatctg aaacaggaat accctgccca gtatcctcaa tgcagatctt cagatcaaac
     2461 attctgtcca aggattcagg gttcaatctc tctgctttca gtctgatgta tccgctgtct
     2521 gtaaatttta cggcatttcc agtcagattc agaagaacct gtctgagtct cattgagtcg
     2581 atcataatgc ttttgggcag tgacgggtca gtttcaatga taaaattcaa gcctttttca
     2641 gaaattttct gagagaatac tgtcttcatc tcttcaagca gttttctgac atcagttccc
     2701 gaaaaattca aatccatttt tcctgattct attttagaca gatccagtac atcattgata
     2761 agtttgagga gaattcttcc gctggtaatg atggatgctg catactgctt cagtacctgg
     2821 tcctccatct tttcattaag aagttcactg aatccaagta tggcattcat cggagttctg
     2881 atttcatgac tcatatttgc caggaattcc gtcttggcgc tgtttgctct gtctgcctct
     2941 tccttggcat tgatcagatt gatctgataa ttttttctct ctgttatatc ctgaaagctt
     3001 gcggcggctc catacattat attgtcatta ttaaacagag gaacagcatg tacggaaagc
     3061 catttccgtt caccgtgccg gttctcaatc atatgctcaa tcggaccgtc agctttctga
     3121 ttttccagaa cggctgccag aggaagtctg ttcagaggaa aaggatcgcc gttttcatca
     3181 agctgcttcc agttttccgc attaaagtat tttcctacaa tatctgattc atatatatcg
     3241 agtatccgtc cagcctcctt attggcatag acaatttctc cctgcaggtt gacctccaca
     3301 attccttccg gaattgagct cagaatctcc ttcagtttgt tctgtgccgt ctgaagagat
     3361 gtttcggttt tctttgtctc tgtaatatca gtaacaagta ctataaatcc ttttactata
     3421 ttgttttcta tatcaggaat atactgcgcc catgtaatgc cggctgaccc gtcgggcttg
     3481 acaatctttc tttcaaatct ctgcatttct cccgccagag ctttttccac atacggaaga
     3541 tttaaattgt acaagtccgc tcccagaaga tctttcattt ccattccgag aacttcctcc
     3601 gagctccttc cgaaccattc cctgtaagcc aggtttgcaa attcacattt tttttcacgg
     3661 ttccagtatc cgatcagacc ggggattgaa tcagtcacct tctgaatgaa atttttctgt
     3721 tcaaacagct tctcctccag ctgctttctg gcagagatat ctcttgtaat cagagtcagc
     3781 tcttttacgc ttcctcctga aaaaatcgga ctgacatgag taaggtacca ggatgatctg
     3841 ccgtctgaat tgataaattt tgtttcataa ctgagaggtt ttcccgtttc agcagactcg
     3901 cggtgtttct gactgaccag atccctgtac tcaggcagaa cgaaatcgca ggcattctgc
     3961 ccgactacct gttcttttgt aaatccttct gcagtcctgc tgataaacag aatcctgttg
     4021 ttcaaatcca ctttcatgat aatatcaggt gcattttcaa cgagggaccg atactgctcc
     4081 tcgctttctg aaagagccct ctccatttcc tttctggcag taatgtcctt tcggatggac
     4141 acatactgat aaatttctcc ttccgcattc aggaaaggca caatgacagt atcgacccag
     4201 tacaaagaac cgtcttttgc tctgtttttc acttcccctt tccagacttt tttgctcaga
     4261 acagtttccc acatttctgc gtaaaactca ggaggatgaa agcctgactt tacaattctg
     4321 tgattgcttc ctatcagctc ttcctgagaa tactttgaca tcctgcagaa ctgttcattt
     4381 gcatatatta tatttccttt actgtcagtc atagcgacta ttgcatactc atcaagagcc
     4441 gcagcaaaat cctttatctt ggcagtggaa tccagaagag caagttccgc ctttttgtac
     4501 tgagtgatgt ccctgagtat gcccctggca gaataaggct ttccgtcccg aaagacacat
     4561 cccccggaac cctgaatata aatctttttc ccgtatttgc tgatgaatgc agattcaaag
     4621 aacggaatgt ttttccctga actcagttct ttcagaattc tcatacattt ttctctgctg
     4681 tccggatgaa ctatatcata tattgtgatt ttttttattt catctgcagt atattctaat
     4741 gattcaagcc atgccttgtt tacataaata aaacgtccgt cagagactct tacattctga
     4801 atcagatcgc ttgtattttc gaaaaattcc ttgaaatcgg attcggactc ttttttctct
     4861 ctctcaattc gttcccttgt ctttctgcct tcttccagct gttcattgct ctgcctgacc
     4921 agattttcca gacttctgtt cagttcctcc agatgttttc ttgcgttttc cctttcatca
     4981 atttcgcgct gggccttttc agaagctgct ttgttcagct tcagcgcact caaggtaatg
     5041 aaagcgattc cgaaaccgat catactctga atcacaagga aattaagcac tgcttcatca
     5101 tttgcataag ggtaggaaat cagtgaattc tgcatcagct tcagcagaac aaatccgtat
     5161 gagggaataa aaacagccgc cgctatggaa actctgggaa gagaaagcag agcagaacct
     5221 gcagtcagga tatgaaatcc ggaaaaagca ggtgccatta ttcctccgtt gatcagcgtt
     5281 gcagtgatca gcactgacca aaatccgaat accaggagga actttgcagc tttaatctta
     5341 ttgcttcgca gaagaaaata tgatatggaa agaaacaggc tcagcagaac aagaatcagc
     5401 tgtcttcttt ctgaaatccc gaaaaacagc atcagaatct ctcccacaac aatgatgact
     5461 gccattaccg acattgatgc tttcagaata agataggaaa attcttcttc ggaaaatgcc
     5521 tgaggtgaag gaattttcat tctttgtctg aatttattat atatatttag aatataagac
     5581 attaaatcag ctctcttcca tattttctgt ccttactgcc ggacggtttt tctgaacaga
     5641 tcagagacag ccttctgatt ttccttttct gcaatatcca tcggagaaag tccttccttg
     5701 tttttctgat catacctgaa tccttcctgc agaagagatg ctgccatatc tgtgtatccg
     5761 cagtatgcag caaaatgaag agctgtgttt cctgctttgg aagatgtctt tctggcaccg
     5821 cccgcaaaca gcagttctgc aatctgcctg tttccggcgt acttatgcgg ggtattctca
     5881 gaactgccct gtctgtgcag aatgcatttt tcgaacgcat agtcaagaac agttctgcct
     5941 gaaaagtctt cagaattgag atttgccccc tgccttatgc agtatcgaac ctcggctgca
     6001 ttgttttcct ttactgcaat atatatatcg tctttataat aaagatattt taaagcagag
     6061 aacggaaggg agacaggaag caaaagagta tccagcacag aactgaaagg ggtatctata
     6121 agacagtaca atgcaccaaa aagagcggca ggtcctgcca ctggtccctg ccctccagga
     6181 atacctgacc agcatgatct tactccgctg tagacaacag gtttgtcctt tctgctccag
     6241 tctttgaaag aggcagcagt actgcatttt t
//
LOCUS       DAOQGJ010000016         6176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_6330_length_6176_cov_3.715243, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000016 DAOQGJ010000000
VERSION     DAOQGJ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6330_length_6176_cov_3.715243"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="PK683_00495"
     CDS             <1..1634
                     /locus_tag="PK683_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06949.1"
                     /translation="DYMKYLLSLLLLIMIGCKAPNDDTKNKDAAAPFLIGTADYYRQL
                     ALDSDVIAANANASAIAAAKALPLLDVSSKTAAELQVLNLDGVISSVDMSKGTPIVTF
                     YVTTKATSALPASRLKGFGLSIKTNGSYNATTGVIQTPGATTVISNLYSVPHMSFSFA
                     KLVVGTNGAPDKWVSYLTTTVPTPDGTIGPSYTAFKRSTPGWQKNSVDQNGTLTDNGD
                     GTYTYTFARDITKSKDYISSEYLVASTENMTAVSAARIAGMGDLKYDNTLPHRLAIAI
                     GIGTVPGSGTANTNKTNQPVMSGGCTSTTSCSGTAVAALNFTYGTTLVYDFIPATSKI
                     KAMQRDIVRVSTCNNCHKDASATTTSGITTRGLIFHGAGGRNDVTQCVTCHNDQTRAQ
                     VESSLASVSGVFTGTNGMVLDGYAVADFPIMVHKFHMSSWLQKSGTGYYVGSTSYMFP
                     TKAAAFITKADSKLCYNCHTTNKTANNEDNWKTRPSRIACGACHDGIDFTTGLMTKSA
                     NPSNTDTTHGGGKQLDDSKCAGCHSSQYIITKHGL"
     gene            complement(1676..3214)
                     /locus_tag="PK683_00500"
     CDS             complement(1676..3214)
                     /locus_tag="PK683_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH06950.1"
                     /translation="MMNELKKRLLKYLLYYSLIGYGTNFFIVIAQNFIMEYLLSVSEH
                     EYFRNGAVNTRAGTAGMVFSFGIPLLIILWYNIPIIKGVFQETDEEKMSIIKRRIINT
                     PIFVAAISFAGWGTSSSVFGWIAYNVGPDGNIILSVKVVFMNVLMSLVCFSMSYYLAD
                     AVNRRIVVPDLFPEGGLSRISGAMKIPIRVRFLILYVSISVTPVFVAGNMVIAGMQKE
                     SDYSRILPTFLILFSVLAIAWILTWMVSNYFTVPVKSITESAVHISAGDYDRTVQIVS
                     NDEIGFLGEKINEMASGLKEKEFIKDTFGKAVDPKVRDHLLKGNISLGGEIKNVTVLF
                     SDIRGFTSVSEQLSPEEIVRWLNRYFNRMSACVNRNGGFVNKFIGDAILAVFGAPAEL
                     ENQAEKALQAALEMRNALVELNNEFAADNIPNIRIGIGIHSGPVIAGNIGSESRMEYT
                     VIGDTVNTASRLEGLCKEFGKDLIISADVKKLISQNFGLSVSAPISVQIRGKTENQEI
                     YFID"
     gene            complement(3507..4685)
                     /locus_tag="PK683_00505"
     CDS             complement(3507..4685)
                     /locus_tag="PK683_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNH06951.1"
                     /translation="MSYTVSTHRQKELVQFRRTIHSNPELMFQEKDTSALVVSHLKKE
                     GFSFESGIAETGVVCLIDSGLPGKTLLVRADMDALPIHEENITEYSSRKKGVMHACGH
                     DAHTSILMGLASDLRQDLKSVIPKGRVLLVFQPAEEGGGGADRMVEAGVLEKYSVDAA
                     LALHVWNHVDIGKVGVVDGTMMASVDEFRITVKGKSGHGAMPQHTVDPVTVSAYIITA
                     LQTIVSRNTDPLDSCVVTVGSLHSGDAFNAIPDEAKMVGTVRTYSKEVYDKVPDILKR
                     IVTKTAESFGASAEVEYIRVNQPTINTPAMADIVRKASRKVLGPDSVTEKETRTMGGE
                     DFSAFLAKVPGCYFFIGSRNEAKGFTCSHHNSKFDFDEDALSIGLAVMKEAVRIFLSD
                     "
     gene            complement(4685..5059)
                     /locus_tag="PK683_00510"
     CDS             complement(4685..5059)
                     /locus_tag="PK683_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH06952.1"
                     /translation="MKGLFYINIRPGRFFAAAVIVLSSLVFCGNGDQNRKDLESKISS
                     GAEVIDVRTVEEFNSGHYPGAVNIPVDQLEGKISSLDKNKAYIVYCRSGRRSGIAQKK
                     MTESGFRNVTNAGGLKDMPEKK"
     gene            complement(5148..>6176)
                     /locus_tag="PK683_00515"
     CDS             complement(5148..>6176)
                     /locus_tag="PK683_00515"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH06953.1"
                     /translation="DEYLRFVLLTGVSKFSKTGVFSGLNNLNDISMSLRYSAIAGITE
                     EELKNNFKEYTAEFSNTVKISEDELLQKIRHWYNGYCFSGQCEKVYNPFSTLLLFENK
                     EFKNYWFETGTPSFLIDLAKEKDFAISELPILAEETSFSTYEVDELDVIPLLFQTGYL
                     TLKGYDPERLLYTLDYPNFEVKNAFLQQFTRRYTNRVFQESKLYGLIDSLRERDFENF
                     FLVLRSIFANIDYDLHIPAEKYYQTIFYLIFTLIGLRVSAEVKTNIGRIDAVIEDRGG
                     IFLFEFKFSGTKEEALAQIRKNKYFEKYLSPAVTYGQTALQREIYLFGVEFSDKNAGE
                     WVMEKMTG"
BASE COUNT         1777 a         1443 c         1197 g         1759 t
ORIGIN      
        1 gggactatat gaaatactta ttatcacttt tacttcttat aatgatcggg tgcaaggctc
       61 cgaacgacga tacaaagaat aaggatgctg ctgctccttt cctcatagga acagcagact
      121 actaccgaca gcttgcactt gactcagacg ttattgctgc aaacgcaaat gcgagtgcaa
      181 tcgcggcagc aaaagcactg ccgcttctgg atgtttcctc taaaacagcg gcagaacttc
      241 aggtattgaa tttggatgga gtcattagca gtgtggatat gtccaaggga acgccgattg
      301 tcacttttta tgtaacgaca aaagccacat cagcacttcc tgcaagccgt ctgaaaggct
      361 ttgggctttc cattaaaacg aacggatcct acaatgcaac tacaggagtc atccagactc
      421 ccggtgcaac tacagtcatt tccaacctat attcagtgcc tcatatgagc ttttcttttg
      481 caaaactcgt tgtaggtaca aatggtgctc ctgacaaatg ggtcagctat ctgactacaa
      541 ctgttccaac ccctgacgga acaataggtc caagctacac agctttcaaa agaagcactc
      601 caggatggca gaagaacagt gtggatcaga acggaacatt gacagacaac ggtgacggga
      661 cgtacacata cacatttgcc agggatatca ctaagtccaa agattatata tcttctgaat
      721 atttagttgc atcgacagaa aatatgacag ccgtttctgc tgccagaatt gccgggatgg
      781 gcgatctgaa gtatgacaat actctccctc acagactggc aattgcaatc gggattggta
      841 cagttccagg atcagggact gccaatacga ataaaacaaa tcagccggtg atgagcggtg
      901 ggtgtacatc cacaacctcc tgttcaggaa ccgcagttgc agccttaaac tttacttacg
      961 gaacaactct tgtatatgat tttatccctg cgaccagcaa gattaaggca atgcagagag
     1021 atattgtaag ggtatctacc tgcaacaact gtcacaagga tgcaagtgca acaacaactt
     1081 cagggatcac gacaagagga ctcatttttc acggggccgg tggaagaaat gatgtcactc
     1141 agtgtgtaac atgtcataat gatcagacca gagcacaggt tgaatccagt cttgcttcag
     1201 tgagcggcgt ttttacagga acaaacggaa tggttctgga cggttatgcc gttgctgatt
     1261 ttccaattat ggttcacaaa ttccatatga gttcctggct gcagaaatcc ggaaccggtt
     1321 actatgtggg atcaactagt tatatgtttc cgacaaaggc agccgcattc ataactaagg
     1381 cagattccaa gctctgttat aattgtcata cgacaaacaa gacagcaaat aatgaagaca
     1441 actggaaaac aagaccaagt cgaattgcat gcggggcctg tcatgacgga attgatttca
     1501 ctaccggtct gatgactaaa agtgcaaatc cttccaatac tgacacgact cacggaggcg
     1561 gaaagcagct ggacgacagc aaatgtgcag gctgtcattc atctcagtac attatcacaa
     1621 aacacggtct gtaatattca gacctttcag gcagtcaaca ctgcctgaat ttttgtcagt
     1681 ctataaaata aatttcctga ttttcagtct tgcctcttat ctgaacagat ataggtgcac
     1741 ttacggaaag cccaaaattc tgtgagataa gtttttttac atctgcactg ataatcagat
     1801 cttttccaaa ctccttgcag agtccttcca gtctggacgc agtattgaca gtatctccga
     1861 tcactgtata ttccatcctg ctttcagatc cgatatttcc cgcaatgacc ggtcctgaat
     1921 gaattcctat ccctattctt atatttggta tattatccgc tgcaaattca ttattcagtt
     1981 caaccaatgc atttctcatt tccaatgcag cctgaagggc cttctctgct tgattctcca
     2041 gttctgccgg tgctccgaat acggcaagta ttgcatctcc aatgaacttg ttgacaaaac
     2101 cgccgttcct gttcacgcag gcactcatcc tgttgaagta tctgttcagc caccttacaa
     2161 tttcttccgg agaaagctgt tcggatacgg aagtaaaccc ccttatgtca gagaaaagaa
     2221 cagtcacatt cttaatctct cctccaagag aaatatttcc tttcagaaga tgatctctga
     2281 ccttaggatc tacggctttt ccgaatgtat ccttgataaa ttctttttcc ttgagtcctg
     2341 aagccatttc attgatcttt tctcctagaa aaccaatctc atcattggat acaatctgaa
     2401 ctgtcctgtc ataatcaccg gcactgatat gaacagccga ctcagtaatg cttttgacag
     2461 gaactgtaaa ataatttgaa accatccaag taagaatcca tgcaattgca agaacggaga
     2521 aaagaatcag aaatgtaggc agtatgcgtg aataatcgga ttctttctgc atccctgcga
     2581 ttaccatatt cccggcaaca aagactggag taacagatat tgaaacatat aatataagaa
     2641 atctaactct gatgggaatc ttcattgcac ccgatatccg agaaagaccg ccctccggaa
     2701 aaagatccgg tactacaatc cttctgttga ctgcatcagc cagatagtaa ctcatggaaa
     2761 agcagaccaa ggacataagc acattcataa atacaacttt tactgacagg ataatgtttc
     2821 cgtctggtcc tacattgtag gcaatccatc cgaaaacaga ggaagatgtc ccccatcccg
     2881 caaaagatat tgccgccaca aatatgggtg tatttataat acgtcttttt attattgaca
     2941 ttttctcttc atctgtttcc tggaatactc cttttataat aggaatattg taccaaagaa
     3001 taatcagaag aggaattcca aaagaaaata ccatacctgc agtgccggca cgggtattta
     3061 cagccccgtt tctgaaatat tcatgttccg atacggacag aaggtattcc attataaaat
     3121 tctgagcaat tacaatgaaa aaatttgtac catatcctat cagtgaatag tacaaaagat
     3181 atttaagcag tcttttcttt aattcattca tcatctctgt gatatttcct tgagctctag
     3241 accgtccggc ggcagcccga aaaacctttt actagtctga aagccagtca aagccggacc
     3301 gattaagtaa tctgcatcag tcactcacgt tccgcctcca gctgcattgg caggaacgtc
     3361 tgcaggcggc ttgaggactt gcaaatctgg aagcagcgaa gcggcgaagc gccgaagcgg
     3421 ctgttctatc cgattcatcg caggctttta aactgcccga tccctctaat gcttcgtaaa
     3481 aaaataggct ccatacatta ggtgagtcag tcagacagaa atattctgac tgcttctttc
     3541 attaccgcaa gaccgatact gagtgcatcc tcatcaaaat caaacttcga attatggtgt
     3601 gagcaggtaa atccttttgc ctcattccgg gaaccgataa aaaaataaca gccaggcact
     3661 tttgcaagaa atgcggaaaa atcctcaccg cccatcgttc tggtttcctt ttcggttaca
     3721 gaatccggcc ctagaacttt ccttgaggcc tttctcacaa tgtccgccat tgcaggggta
     3781 ttgattgtag gctgattgac ccgaatatat tccacttctg cggatgcacc gaaagattct
     3841 gcagtcttag tcactatcct ttttagaata tcagggactt tatcatatac ttctttagaa
     3901 taagttctga cagtgccgac catttttgcc tcatcgggaa ttgcattgaa tgcatcgcct
     3961 gaatgcaggc tgcctacagt cactacgcag gaatccaggg gatcagtgtt tcttgaaaca
     4021 attgtctgaa gtgccgtaat gatgtatgcg gaaacagtga caggatctac tgtatgctga
     4081 ggcattgccc cgtgcccgct ttttcctttt actgtaatcc gaaactcatc cacagaagcc
     4141 atcatagtac cgtctacaac accgactttc ccgatatcca catggttcca tacgtgaaga
     4201 gcgagagctg catccacact gtacttttcg agtacccctg cttccaccat cctgtctgct
     4261 ccgcccccgc cttcttctgc cggctgaaac acaagaagga ctcttccctt tggaatgacc
     4321 gacttcagat cctgccggag atcggaagca agccccatca gaatggatgt gtgagcatcg
     4381 tgaccgcagg catgcatgac tcctttttta cgagaagaat actctgtaat gttttcttca
     4441 tgaattggga gtgcatccat atccgctctc acaagcaggg tttttcccgg aagtcccgag
     4501 tcaatgaggc agaccacacc agtctctgca attcctgatt caaaactgaa tccttccttt
     4561 ttcagatgag acactaccag tgcagatgta tccttttcct gaaacatcag ctcaggattg
     4621 gagtgaatcg ttctcctgaa ctggacgagt tctttttgtc tatgtgtgct tacagtatat
     4681 gacattattt tttctcaggc atatctttca gtccgcctgc attcgttaca ttcctgaacc
     4741 cgctctcggt catttttttc tgagcaatcc cggatcttct gccggatctg cagtaaacga
     4801 tataggcctt gtttttatcc agtgaagaaa ttttcccttc cagctgatct acaggaatat
     4861 tgactgcacc aggataatgt ccgctgttga actcttcgac tgttctgaca tcaatgactt
     4921 ctgctccgga gctgatttta ctctccagat ctttcctgtt ctgatcaccg ttcccgcaga
     4981 aaacaagtga gcttaagacg atgactgctg cggcaaaaaa tctgcctggt ctgatgttta
     5041 tgtaaaataa tcctttcata agatctccaa tctacaatat ctccaattgg aaaatttgag
     5101 tcatgatctg tcaagaggaa agaaaagcag ttttcggaag gaaaaaatca gccggtcatt
     5161 ttctccatta cccattcgcc tgcgttttta tcagagaatt ccacgccgaa taaatatatt
     5221 tcgcgctgta atgctgtttg accatacgtc actgcaggcg ataaatattt ttcgaaatat
     5281 ttattttttc tgatctgtgc cagtgcttct tctttggttc cgctgaattt gaattcaaaa
     5341 agaaaaatcc cgcctcggtc ttcgatcaca gcatcgattc tgccgatatt cgtttttact
     5401 tccgcagaaa ccctaaggcc gattaatgta aatatcagat aaaatattgt ctgatagtac
     5461 ttctccgcag gtatatgcag atcataatct atgtttgcaa agatagaacg cagaaccaga
     5521 aaaaaatttt caaagtctct ttcccgaaga ctgtcgatta aaccgtacag tttcgattcc
     5581 tgaaatactc tgtttgtata cctgcgggtg aactgctgca gaaacgcatt ctttacttca
     5641 aaattcggat aatccaatgt gtaaagcagc ctctccggat cataaccttt caatgttaaa
     5701 tagcccgtct gaaagagcag cggaattaca tcgagttcat ccacttcata ggtggaaaag
     5761 gatgtttctt ccgccagaat cggcagttct gaaattgcga aatctttttc cttggccaga
     5821 tcaatcagaa aacttggagt ccctgtttcg aaccagtaat tcttgaattc tttattttcg
     5881 aataaaagca aggttgaaaa agggttgtaa actttttcac attgtcctga aaaacaatac
     5941 ccgttatacc aatgacgaat tttctgcaga agctcatctt ctgaaatttt tactgtattt
     6001 gaaaattctg ctgtatattc tttaaaatta ttttttaatt cttcttcagt aattccggca
     6061 attgcagaat acctcagact catagaaata tcatttaaat tgttcaggcc gctgaacacc
     6121 cccgtcttcg aaaacttgct gacgcctgtt aacagcacaa aacgtaagta ttcatc
//
LOCUS       DAOQGJ010000017         6104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_6428_length_6104_cov_3.150603, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000017 DAOQGJ010000000
VERSION     DAOQGJ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6428_length_6104_cov_3.150603"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PK683_00520"
     CDS             complement(<1..197)
                     /locus_tag="PK683_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH06954.1"
                     /translation="MAVPKVARLDVIDKNKVFDLIVLGGGVTGANILWDAALRGLDAV
                     LFEKNDYASGTTQATSKLIHGG"
     gene            complement(197..1120)
                     /locus_tag="PK683_00525"
     CDS             complement(197..1120)
                     /locus_tag="PK683_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNH06955.1"
                     /translation="MSYEKIHFIINPASGGGSTGKTWAQMRSYIESKLGTFSFEFTAN
                     KGHGIDLAKRAAEKSVEKIVAIGGDGSISEIVSGILLSGNTNAVIGVLNLGTGGDFCR
                     TLGIPGDIKLGLEKIRSGSVISSDIGKVSFTDNSGSLAMRSFINIAGCGMSGEVVRTI
                     NQSKKIFGGFSYYVSSLKNLFSYRNKRVRILADEMPEKMFTSVTVAVCNGQYFGGGMR
                     VAPNAQISDGMFDVVIMEDWNLWEKLKYSPRLYNSTILSCPKITHFRAKKIVILPQEG
                     ENEVYIDCDGEDVGTIPMTLEILPSAVRFTV"
     gene            complement(1169..2761)
                     /locus_tag="PK683_00530"
     CDS             complement(1169..2761)
                     /locus_tag="PK683_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06956.1"
                     /translation="MKISLLIALLSFAGCSSGMSKSVQNEFSGSKMKISFQGRLNSLP
                     SVLKGKPLRVESRFYRCTETDFGKLDADRGIYPRKKDDCRDRFISSSFSFPSEQTYKF
                     EQEISSGWTHGFIRLLDIEEVSGKYSPGDNPFWFSPDDLSAGNSISHDFSFISMKNPI
                     PGKKQEELAAAFAPVIVMKKGKKFYPSNLEKYHKKYRVEKHFGKKPDSGFYEVPDPAM
                     DQYLVLDESLYGTGDTHLYYHVRPADVSVSGASPKALPGWRDNRNYRYQAGASGETVI
                     SYYFWYDYNEGPTSFGNSHEGDLESFAVLLDRSGKPKRVMATGHNHVMIDTVWENINS
                     FRNHPVLYIAHGNKGADGGNPTSFYGGHTVSLDAGNSLFTFLTSPKDIFPEIGNESEL
                     ILPADLKKEDLVRLRIGPGEWIDKNTKYLDASPYVSRYVKKLVQWPEPGWIGEDSSGQ
                     NADESLSEIFRFRGRLGRHPRSSLNIFELKQYGKSPVNVPFKMNDEQHFTLEKPQTDR
                     CENGLSGDYCPKFRGDRRTPQF"
     gene            complement(2761..3891)
                     /locus_tag="PK683_00535"
     CDS             complement(2761..3891)
                     /locus_tag="PK683_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06957.1"
                     /translation="MQSSVKMKKRYILIIILSFCTVQCRKQSEVEKIDVLLSSEKFSK
                     AEETARSRLSTKHSNDEYLSKEPAKDRSVQFSNDRNRVVWTEGKQLQFRDLANPLSKT
                     IGLNETAENLSVSSDAEHALISVSAANGTACRLLAVSILEKKKSYLSESFVHCKNQFG
                     ISKDGTRIYYIMDSSLYTEETSDTKNHKVLLDKKHFPETVHSVRNRYYFRPVGHNFLI
                     FSGDAGAYNVYWLDVRKNALEKITDAASPLFYYGSGKNAYFIRGHIGSLSAQEISFSA
                     YGRPVLSDSFAVNVRETVLWPVSENNEFVSLVDGKAVLWAPGGKKKELPLLAKHIYLS
                     ARDNIIYETSDGYLMKTSMDFSEEDWKALELYKKTKEKTGEK"
     gene            complement(3899..4495)
                     /locus_tag="PK683_00540"
     CDS             complement(3899..4495)
                     /locus_tag="PK683_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06958.1"
                     /translation="MEKTISDRMYSILMDADQYQSPYAKSIGEPDEILFPLIRKYCLQ
                     TAAVSAFSSVPAGTAGIMTVFPEVFALLKLQSRMVKDIAAVYGKDKSLSKEVMLYCIF
                     KKSGSNLFERFIRMSSSRVIVRPVSIRTFADFLQKWSPRLAGKFFGKGLSRILPWAGI
                     VFGGAAGYLDTRIVAITSINLFSRDILVMEEENELEEI"
     gene            4600..5664
                     /gene="prfA"
                     /locus_tag="PK683_00545"
     CDS             4600..5664
                     /gene="prfA"
                     /locus_tag="PK683_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNH06959.1"
                     /translation="MRDRLLKIRQKYLKIDEELSNPQNLKPGQLKDLSKERSRLTPIF
                     DKIEEYLKTEKNLDDAKSLLKTESDSDMISMLKGEVQSSETLLNSLSEELEIMLLPPD
                     PNSGKNILVEIRAGTGGEEAALFVADIFRMYSKYLEKHKMKYETVEAAPTGLGGFKEI
                     VFSIQDETAYDTFKFEPGAHRVQRIPVTESGGRIHTSAITVAVLPEVEESELHIAENE
                     IRIDVFRSSGPGGQSVNTTDSAVRITHIPTGIVVSCQDEKSQLKNKDKAMRILRARIQ
                     EKREEESKANSDALKKQMVGSGDRSERIRTYNFPQGRMTDHRINYTSHNLSAVMDGDL
                     DDLFKELIMQDRIKRLQENR"
     gene            5702..>6104
                     /locus_tag="PK683_00550"
     CDS             5702..>6104
                     /locus_tag="PK683_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HNH06960.1"
                     /translation="MRRIRSADTKPEIKIRSELHKAGLRFRKNSNKILGKPDIVFHGY
                     RTLVFVHGCFWHRHKNCKKATTPSTNREFWEKKFERNIQRDRETVKSLKKDGWKVFII
                     WECEVNRPSKINRLIKKIKDFKKKTGNFSITG"
BASE COUNT         1799 a         1375 c         1240 g         1690 t
ORIGIN      
        1 cccccgtgga tgagcttgga agttgcctga gtcgtgccgg atgcatagtc atttttttca
       61 aagagaacag cgtcaagtcc gcgaagcgcc gcatcccata aaatgttggc accagtgact
      121 cctcctccca aaacaatcag atcgaagact ttatttttat ctattacatc cagtcttgcg
      181 actttaggaa cagccattat actgtaaacc tcactgcaga aggaaggatt tccagggtca
      241 taggaattgt cccgacgtcc tctccgtcgc agtcgatata aacttcattt tccccttcct
      301 gaggaaggat gacaattttt tttgccctga aatgagtgat tttaggacag cttaatatag
      361 tactgttata taatctgggt gaatacttta atttttccca gagattccag tcttccataa
      421 tcacaacatc aaacattccg tccgatatct gtgcattcgg tgcaactctc attcccccgc
      481 cgaaatactg accgttgcat actgcaactg tgactgaggt gaacattttt tcaggcattt
      541 cgtctgcaag aatgcggact cttttgtttc tgtaggaaaa aagattcttc agactgctga
      601 cataatagga aaatccgccg aatatttttt tggactggtt gattgttctc acaacttcgc
      661 ctgacattcc gcatcctgca atattgataa aacttctcat tgcaagactg cctgaattgt
      721 ctgtaaaact cacctttccg atatcggaac tgatgactga tccgctcctg attttttcca
      781 gaccgagttt gatgtctccc ggaattccga gtgttctgca gaaatctccg ccggttccca
      841 gattcagtac accgatcact gcatttgtgt tcccggagag aagaatcccg cttactatct
      901 cggaaatgga tccgtctccc cctatggcca cgatcttctc aacactcttt tcagcggctc
      961 tttttgccag atcaattcca tggcccttgt tcgcagtaaa ctcaaaagaa aaagttccca
     1021 gcttgctttc aatgtaggat ctcatctgag cccatgtttt gcctgtgctt cctccgccgg
     1081 aagcggggtt aatgataaag tggatcttct cgtaactcat ttaaacaaga aatttcacag
     1141 aacccgtgac gggcaaatat tttttgtttc agaactgggg agttcttctg tctcctctga
     1201 acttgggaca gtagtcgccg gaaagaccgt tttcacatct gtcagtctgg ggtttttcca
     1261 gagtaaaatg ctgctcatca ttcattttaa acggcacatt gaccggtgat ttcccgtact
     1321 gcttcagctc aaaaatatta agactggaac ggggatgcct tccaagtctt cccctgaatc
     1381 tgaatatctc agacagagac tcatctgcat tctgaccgga tgaatcctct cctatccatc
     1441 cgggctccgg ccactgtacc agttttttca cataccttga aacgtaagga gatgcgtcca
     1501 gatactttgt atttttatca atccattctc caggaccgat ccgaagtctg acaagatctt
     1561 cttttttcag atcggccgga agaatcagtt cagactcatt tccaatctca ggaaatatgt
     1621 ccttaggaga agtcagaaac gtaaaaagag aattgcccgc atccagagat acagtgtgtc
     1681 cgccgtaaaa ggatgtagga tttcctccgt ctgcaccctt gtttccgtgg gcaatataaa
     1741 gaacaggatg atttctgaat gaattgatat tttcccatac tgtatcaatc attacatggt
     1801 tgtgccctgt ggccatgact cgtttaggct ttccgcttct gtcgagaaga actgcaaagg
     1861 actccagatc gccttcatga ctgtttccga aggaagtcgg tccttcatta taatcatacc
     1921 aaaaataata ggaaatcaca gtttcaccgg aagcacctgc ctgatacctg tagtttctgt
     1981 tgtccctcca tccgggaagc gccttgggag atgctcctga aacggaaaca tccgcaggtc
     2041 tgacatggta gtacagatgt gtatcacctg ttccgtacag ggattcgtca aggacgaggt
     2101 attgatccat tgcaggatca ggaacctcat aaaatccgct gtcaggcttc ttcccgaaat
     2161 gcttttccac tctgtatttt ttatgatact tttccagatt ggacgggtag aactttttgc
     2221 ctttcttcat tacaatgact ggagcaaatg ccgcagcaag ctcttcctgt ttttttccgg
     2281 gtatcggatt tttcatggaa ataaatgaaa aatcatgaga aattgaattt cctgcagaaa
     2341 gatcatccgg agaaaaccag aaaggattgt ctcctgggga atactttcct gaaacttcct
     2401 caatgtccag aagccggatg aaaccgtgcg tccagcccga ggaaatctcc tgttcaaatt
     2461 tataggtctg ctcggaagga aagctgaaac tggaagatat gaacctgtcc ctgcagtcat
     2521 ctttttttct tggataaatc cctctgtctg catcgagttt tccgaaatca gtctcagtac
     2581 atctgtagaa tctggactcc accctgagcg gttttccttt cagaacggaa ggcagagaat
     2641 tcagcctgcc ctgaaatgaa attttcatct tactgccgga aaattcattc tgtactgatt
     2701 tcgacatccc tgatgaacag cctgcaaagc tcaatagggc aatcaataat gaaattttca
     2761 ttatttttct cctgtttttt ccttagtttt tttataaagt tccagtgctt tccagtcttc
     2821 ctcggaaaag tccatcgagg tcttcatcag atatccgtcg gatgtctcat agatgatatt
     2881 gtctctcgca ctcagatata tatgttttgc aagaagggga agctcctttt tttttccgcc
     2941 gggagcccag agaaccgctt tcccgtctac aagggataca aattcattat tctctgatac
     3001 aggccagagg actgtctccc tgacatttac tgcaaacgaa tccgaaagaa ccggtcttcc
     3061 atatgcagaa aaactgattt cctgtgcact cagactgccg atatgacctc tgataaagta
     3121 tgcatttttc ccgctcccgt aataaaagag aggactggcg gcatccgtta ttttttcaag
     3181 agcgttcttg cggacatcca gccagtatac attgtaagct ccggcatcgc cggagaatat
     3241 cagaaaatta tgaccgacag gacggaaata gtatctgttc cttacagagt gaacagtttc
     3301 agggaaatgt tttttatcca gaagaacctt atgatttttc gtgtcggagg tctcttcagt
     3361 gtagagactg ctgtccatga tataataaat tcgggttccg tcctttgaaa ttccaaactg
     3421 atttttgcag tgaacaaaac tctcgcttaa atatgatttc ttcttttcca ggatggagac
     3481 tgccagcagt ctgcacgcag ttccgtttgc agcagataca gaaatcagtg catgttctgc
     3541 atcggaagag acactcagat tttctgcagt ttcattcaga ccgattgtct ttgaaagagg
     3601 attagccaga tctctgaact gaagctgctt tccctccgtc cataccaccc tgttcctgtc
     3661 attggaaaac tgaacagatc tgtcctttgc cggttccttg gaaagatact catcattgga
     3721 atgtttggtg gaaagtctgc ttcttgccgt ttcttccgct ttgctgaact tttctgaact
     3781 cagaaggaca tcaatcttct cgacctcaga ctgctttctg cactgaacag tgcagaaaga
     3841 aagaataatt attaaaatat atcttttttt cattttaaca ctggactgca ctcagttttc
     3901 agatctcttc aagttcattt tcctcttcca tgacaagaat gtctctggaa aaaagattta
     3961 tgcttgtgat ggcaacaatt cttgtgtcaa gatatccggc cgcacctccg aagacaatgc
     4021 ctgcccaggg cagaatcctg gagagtcctt tcccgaaaaa tttgcctgca agccttgggc
     4081 tccatttctg aaggaaatct gcaaaagttc ttatgctgac agggcgcaca atgactctgg
     4141 aagaagacat cctgataaaa cgctcaaata gattcgatcc tgactttttg aatatgcagt
     4201 agagcatcac ttccttggaa agactcttat cctttccgta cactgcggca atatccttta
     4261 ccattcggga ctgaagtttc aggagagcga acacttccgg aaaaactgtc ataattcctg
     4321 cagtaccggc aggaacagaa gaaaatgcgg aaacagcagc tgtctgcaga cagtatttcc
     4381 gaatcagagg aaaaagaatt tcatccggct cgcctatgct ttttgcataa ggagactgat
     4441 actgatccgc atccatcaaa atggaataca ttctgtccga aatagtcttc tccaaaaccc
     4501 ctccttagta agaagatctt tccctcatcg gaaaatcctg tcactgctta aatactattt
     4561 tactgtaaaa aaaaagacca gttttttttc tatcctaaca tgagagacag acttttaaaa
     4621 atccgccaaa aatatctgaa gatagatgaa gaactgtcta atccgcagaa tctgaaacct
     4681 ggacagctga aagacctttc caaagaacgt tccagactga cacccatttt tgataaaata
     4741 gaagagtatc tgaaaacgga aaagaatctg gatgatgcaa aatctcttct taagacagaa
     4801 tcggattcag atatgatctc aatgcttaaa ggagaagtgc aaagttctga gactctctta
     4861 aattcactct ccgaagaact ggaaattatg ctcctccctc ccgatccaaa ttcagggaag
     4921 aacatacttg tggagatcag ggcaggcact ggaggagaag aggctgcact gtttgtcgca
     4981 gacatattca gaatgtactc caaatatctg gaaaaacata aaatgaaata tgaaactgtg
     5041 gaagcagctc ctactggact cggcggattc aaggaaatcg tattctccat tcaggacgag
     5101 actgcctacg atacattcaa atttgaacct ggagcacaca gagtgcagag aatacctgtt
     5161 accgaatcag gaggaaggat acatacgagc gcaattacag tcgcagtcct tcccgaagtg
     5221 gaagaatcag aactgcatat tgcagaaaat gaaatcagaa tagatgtatt cagatcctca
     5281 gggccaggag gacagtctgt aaatacgact gattcagcag tcagaatcac ccatatcccg
     5341 actggaattg tagtcagctg tcaggatgaa aaatcgcagc tgaaaaacaa ggacaaggca
     5401 atgagaatcc ttcgtgcgag aatccaggaa aaaagagaag aagaatcaaa agcaaattct
     5461 gatgcactga aaaaacagat ggtaggaagc ggagacagat cagaaagaat caggacctat
     5521 aattttcctc aaggcaggat gactgatcac agaatcaatt acaccagcca taacctttct
     5581 gccgtcatgg atggggacct ggatgacctt ttcaaagaac tgattatgca ggacagaatc
     5641 aaaagacttc aggagaacag ataaactgga ccatctgaat cccgaaaaaa gatctgagaa
     5701 tatgaggcgg atccggtctg cagacacaaa accggaaata aaaatcagat ctgaacttca
     5761 taaggcggga cttagattca ggaaaaattc taacaaaata ttaggaaaac ctgatattgt
     5821 atttcacgga tacagaactc tggtctttgt acacggatgt ttctggcatc gtcataaaaa
     5881 ctgcaaaaaa gcaacaacgc cctccacaaa cagagaattc tgggaaaaga aattcgaaag
     5941 gaatattcaa agagacagag aaacagtcaa aagtttaaaa aaagacggat ggaaagtatt
     6001 cattatctgg gagtgcgaag tgaacagacc aagtaagatt aataggctga ttaaaaaaat
     6061 taaggacttt aaaaaaaaga ccggtaattt ttccatcacc ggtc
//
LOCUS       DAOQGJ010000018         6077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_6481_length_6077_cov_3.427101, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000018 DAOQGJ010000000
VERSION     DAOQGJ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6481_length_6077_cov_3.427101"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PK683_00555"
     CDS             complement(<1..511)
                     /locus_tag="PK683_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06961.1"
                     /translation="MIHATASYEIFLSQLKEIYKFSDLVLHSGPLLEENLNRMENDSK
                     NAIPLKPVFLKDICTESHLRKCLSTYQFSLSKYIFLSTFTFFESYISSVITELFEFHG
                     GAEELKRLSTLRSTLRDYDSNIDIEKCRKKLQEYPNKKESFKYIKYTKELERSSFKFP
                     SEILAEYGID"
     gene            complement(524..1498)
                     /locus_tag="PK683_00560"
     CDS             complement(524..1498)
                     /locus_tag="PK683_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNH06962.1"
                     /translation="MNSNEILSLIHKAMDAEIESLQSFRKTVGAEFAEAAQMIYSSEG
                     KVVLTGVGKSGDIAKKISSTMTSTGTEAIFLHPTDAAHGDAGGIRKDDVVLAIGKSGE
                     SDELLFLIPTIKRIGAKLIALTANPGSRLALAADLLLHTPVEKEACPLDLAPTSSTTI
                     ALLAGDALAMALMEMRNFKAEDFALYHPSGRLGKRLSLNIEDVMRKNDSVPKASPEAK
                     LEEILNEITGKYLGAVCITDGENSLLGIITDYDLRKRLSEGSLSKDLQADEMMNKNPI
                     RFQTGRKAYDVLKEMEDRKRPISVAPIVDEQGKLAGIVSLHDLLQKGL"
     gene            complement(1485..1952)
                     /locus_tag="PK683_00565"
     CDS             complement(1485..1952)
                     /locus_tag="PK683_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06963.1"
                     /translation="MNFTVCILISILLHLIFFWNWKKSDRLDIHVKVNRGISISLQNI
                     SLNPAGTKGDSQLSKISGDPGAVSNEIERFKNSLTYPEDAVPQELETDCTWKLILEDG
                     AVREIISIQKCRYGIFDSHFRKSAKEWKFQLAGKQELTVPVSFKIKGTANEQQ"
     gene            complement(1969..4353)
                     /locus_tag="PK683_00570"
     CDS             complement(1969..4353)
                     /locus_tag="PK683_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH06964.1"
                     /translation="MKFAEALGDAEGRTAKVLDAVVKEEISDSSSEYEFSVLCEGKNS
                     PLSKYFHILENIDESMPKDWLSVIKAFPFFTPAYNELFDCALETDDPKTAEYALNLFY
                     NDYSFNGNYKLLKKASKYLIENAEKKFSKKEIWPVVKAHSEKRLLGKKWYDAAISLKK
                     DDPLKSFNALKNAAYHIFEETEEMPSFIINSLISSSKILKDKNLSAIFEEIRPKDKKE
                     EGGKFWKLYEDAEKEYSESEALKDSVKPLFEAVKKNDADSVKKIISSGISPDIQEKET
                     GRTALHYAAEKGFKEIAELLLENQVNSGIKMATGLNLPVHLAAYNGQTDIIKLLVSKG
                     ADIHAVNGRTETPLFLAAQNGHMETVKFLHSKGADLNQKNISQETPIIIAAYNDHDET
                     VKYIAENGGNVNAKDYAGQTALHWAAAAGKTELVKFLIKKGADPKIKDDSNETAADWA
                     RKEYPEISAILEGKKSAEKKEKQISVNEEDILSSLAEEWNMPVKEVKKMDTFHAPYSD
                     IKDESLQHLHLLPKLKTVTLLGCTGLTDAGMKFIAQCRGLEEAHLSGVPITDKGLEYL
                     NTCSSLKKLWIDGTKVSRSGIKKISKLTKLEWIRLNGLSLIDSSIEELKSLKNIKYFE
                     ASDTDITDRLCKLLGTWKNLNLLDLSSAKISSEYLKELSVLKKLQTLRLDNAVIHDDI
                     WKNISSLSALENLSVSGTKLTGKGASSVYSLKKLLHLNLNETDVSDENISEIASLKSL
                     RVLELEKTKITDKSVPLLKTMKNLNALIITGTKISSSGKKALKAAMDPHVSFSW"
     gene            complement(4370..4741)
                     /locus_tag="PK683_00575"
     CDS             complement(4370..4741)
                     /locus_tag="PK683_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06965.1"
                     /translation="MRTETSEHLKSAAALLKYIGSDKNPMDEVWDDMRFHGVYTGNFE
                     DPLQNFGGLGFLPFSGYSDGDLMGLYPVIGQGLENWPVIRVPHSGDPAEVIASSPASL
                     VSIFICRTILNFGGRRTGRLG"
     gene            complement(4830..5324)
                     /locus_tag="PK683_00580"
     CDS             complement(4830..5324)
                     /locus_tag="PK683_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06966.1"
                     /translation="MRDYSRNSALASDRWGKTSGNVFINTSSLNGIGVNTDGLGITSA
                     ANTDLTQISFMITLLADSQASGMTMTGSETGACSRFIFLNTASYGNSESGANQLSMGG
                     TPIDYSCFTGKFLLESTANSCNSTNGNSAGYSNSSPCTAGGSSDFTEQSFSIGASDMI
                     GQTK"
     gene            complement(5339..5680)
                     /locus_tag="PK683_00585"
     CDS             complement(5339..5680)
                     /locus_tag="PK683_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06967.1"
                     /translation="MILQISAQNVTVSSEECFNITWFGGAAGNAGKTVKGKLKQDPYI
                     IKYDWKNGVMNYDWLNDGGRVTGIQGTWKQDGSNGSFILTKNGENYSGFWSSSGNSKK
                     NKLTFAKIECK"
BASE COUNT         1557 a         1282 c         1176 g         2062 t
ORIGIN      
        1 tgtctattcc gtattctgca agtatctcgc ttgggaactt aaaactgctc ctctcgagtt
       61 ccttcgtata ttttatatac ttaaaactct cttttttgtt cggatattcc tgaagttttt
      121 tcctgcattt ttctatatct atatttgaat cataatccct aagtgttgaa cggagagtgg
      181 aaagcctttt cagttcctct gctccgccat gaaattcgaa gagttccgtg attacagaag
      241 atatatatga ttcgaaaaag gtaaatgtac tcagaaaaat atatttagac agtgaaaact
      301 gatatgtact gagacatttt cttaaatggg attctgtaca gatgtctttc agaaataccg
      361 gtttcagggg aatcgcattt ttactgtcat tctccattct gttcagattt tcctccagaa
      421 gaggaccgga atgcagaacc agatcggaga atttataaat ctctttcagc tgcgagagaa
      481 atatttcata acttgcagtt gcatgtatca tttataaaaa aaattacaat cctttctgca
      541 gaagatcatg aaggcttaca ataccggcaa gctttccttg ttcgtcaaca atcggggcta
      601 ctgatattgg tctttttctg tcttccattt ccttcagcac atcataagct ttcctgcctg
      661 tctgaaacct tatcggattt ttattcatca tttcgtcagc ctgaagatcc tttgaaagag
      721 atccctcgct cagtcttttt ctcagatcat agtcagtaat gattccaagg agactgtttt
      781 ctccgtctgt tatacatacg gctccaaggt attttcctgt gatctcattg agtatttctt
      841 ccagttttgc ctcgggagaa gccttgggta cagaatcatt ttttctcatt acatcttcta
      901 tatttaggct cagtcttttt ccgagtcttc ctgaaggatg atagagtgca aagtcttctg
      961 ctttgaaatt tctcatttcc ataagggcca ttgccagagc atctcccgca agaagagcaa
     1021 ttgttgtgct ggaagtcggt gcaagatcga gaggacaggc ttccttttca acgggggtat
     1081 gaagaagcag atctgcagca agggcaagtc tggagccggg atttgcagtc agagcaatca
     1141 gcttggcacc gattcttttt atggtcggaa tcaggaaaag cagttcatca ctctctccgc
     1201 ttttgccgat ggcaagaact acatcgtctt ttcttattcc gcccgcatct ccatgtgctg
     1261 catcagtcgg atgcagaaaa attgcctcgg ttccagtgga tgtcattgtg gaagaaattt
     1321 tttttgcaat atcacccgac tttccgaccc cggtaagaac aacctttcct tcggaagaat
     1381 aaatcatttg agccgcttcg gcaaattcag caccgactgt ttttctgaaa ctctgcagtg
     1441 attcgatttc tgcatccatt gccttgtgaa tcagtgatag gatttcattg ctgttcatta
     1501 gctgtcccct ttatcttaaa acttacaggt acagtcagtt cctgttttcc tgcaagctga
     1561 aacttccatt cttttgcgga ttttctgaag tgtgaatcaa atatcccgta cctgcatttc
     1621 tgaatgctta ttatttccct gactgctccg tcttccagaa tcagtttcca agtgcagtct
     1681 gtttccagtt cctggggaac ggcatcctct ggataagtca aggaattttt aaatcgttct
     1741 atctcatttg aaaccgctcc cgggtctccc gatatttttg agagttgaga atcacctttt
     1801 gtgcctgcag gatttaggga tatattctgc agagagatgg atatcccccg attcactttt
     1861 acatgaatat ccagtctgtc agattttttc cagttccaga agaaaatcag atgcagcaga
     1921 attgaaataa gtatacatac agtaaaattc atttcaattc tgaagattct accagctgaa
     1981 ggaaacatga ggatccattg ccgccttcaa agccttcttt cctgaagaac ttattttcgt
     2041 tccagtgata ataagtgcat tcagattttt catagtcttc agcagaggaa ctgacttgtc
     2101 cgtgattttt gttttttcga gctcgagtac tctcagagat ttcaatgaag caatttctga
     2161 aatgttttca tctgaaacat ctgtctcatt cagattcaaa tgaagaagtt ttttcagaga
     2221 gtaaacagat gatgctcctt tacctgtcag ttttgttccg gaaacactta aattttcaag
     2281 agcagaaaga ctggatatat ttttccatat atcatcatga atcactgcat tgtccagacg
     2341 aagggtctga agcttcttca gtacagaaag ttctttcaga tactctgaac tgatctttgc
     2401 agaagaaagg tccagtaaat tcaaattttt ccaggttccg agcagcttgc aaagtctgtc
     2461 ggtgatatcg gtatctgatg cttcaaagta ttttatgttc ttcaaagatt tcagctcttc
     2521 gattgaagag tcaattaggg acagaccgtt cagtcggatc cattccagtt ttgtcagttt
     2581 ggaaatcttt ttgattccgc tcctgcttac ctttgttccg tctatccaaa gttttttcaa
     2641 agaactgcag gtgttcagat attccagtcc tttatcagta attggtacac ctgacagatg
     2701 cgcctcttcc aggcctctgc actgtgcaat aaatttcatt ccggcatctg tgagtcctgt
     2761 gcatccaagg agagtgactg tcttcagttt gggcagaagg tgcagatgct gcagactttc
     2821 atcttttatg tctgagtaag gtgcgtgaaa tgtatccatt ttttttactt ccttcacagg
     2881 catattccat tcttctgcaa gagagctgag aatatcttct tcattaacac tgatctgttt
     2941 ttcttttttt tcagcagatt tttttccttc gagaattgct gagatttccg gatattcctt
     3001 tcttgcccag tcagccgctg tctcatttga atcatccttg attttgggat cagctccttt
     3061 ttttatcaga aattttacga gttcagtctt tccggctgcg gctgcccaat gcagtgctgt
     3121 ctgtccggca tagtcttttg cgtttacatt tcctccgttt tcagcaatat attttactgt
     3181 ttcatcatga tcgttatacg ctgcaatgat aatcggagtt tcctgactga tatttttttg
     3241 attcagatca gctccctttg aatgcagaaa ttttacagtt tccatatgtc cattctgagc
     3301 cgcaaggaaa agtggtgttt ctgttcttcc gttgactgca tgaatatctg ctccttttga
     3361 aacaagcagt ttaataatgt ctgtctgacc gttgtaagca gccagatgca ccggcagatt
     3421 cagaccagtt gccattttga ttcctgaatt cacctggttt tccagaagaa gttccgcaat
     3481 ttctttgaat cccttttctg cggcataatg gagagctgtt ctgcccgtct ccttttcctg
     3541 gatgtccggg ctgattcctg aagatattat tttttttacc gagtcggcat cattcttttt
     3601 tacagcctca aagagaggtt tgactgaatc tttcaacgct tctgattcgc tgtattcttt
     3661 ttctgcgtct tcatagagtt tccagaattt tcctccttcc tcttttttat cttttggacg
     3721 gatttcttcg aaaattgcag aaagattttt gtccttaaga atttttgaag aagaaataag
     3781 gctgtttata ataaaagaag gcatttcctc tgtttcttcg aatatatggt atgctgcatt
     3841 ttttaaagca ttaaatgatt ttaacggatc atcctttttc agtgatatcg cagcatcgta
     3901 ccattttttt ccgagaagtc ttttttctga gtgtgctttg acaaccggcc agatttcttt
     3961 cttactgaat tttttctctg cattctctat cagatatttc gaagcttttt ttaataattt
     4021 ataattgcca ttaaatgaat aatcattata aaaaagattc agtgcatatt cagctgtttt
     4081 aggatcatcc gtctccaatg cgcagtcgaa gagctcattg taagccggag tgaagaaggg
     4141 aaaggctttg atgactgaaa gccagtcctt aggcatagat tcatcaatgt tttccagaat
     4201 atggaaatat tttgaaagag ggctgttttt tccttcacat aaaactgaga actcatattc
     4261 agaggaactg tctgagattt cttctttaac gactgcatcc agaacttttg cagtcctgcc
     4321 ttctgcatca ccgagagctt ctgcaaattt cattattttc tttctgtttt tatccaagtc
     4381 ttccagttcg tcgccccccg aaattcagaa tagttctgca gatgaatata gaaacaagag
     4441 aagcaggaga tgaggcaatg acttcagccg gatctcctga atgggggaca cggatgacag
     4501 gccagttttc gagtccttga ccgatcactg gatacagtcc catcagatca ccgtcagaat
     4561 atccggaaaa aggcaggaat cccagccctc cgaaattctg gagaggatct tcaaaatttc
     4621 ctgtatacac tccgtgaaac cgcatatcat cccaaacctc atccatagga tttttatcgc
     4681 ttccaatata tttcaaaagt gcagctgctg attttaaatg ctcagaagtc tcagttctca
     4741 tagtcccgcc tgtctctcgt tgttttcatt gtaatcagaa tggaactgaa tgaattgtca
     4801 atatttaata gaaattattc aatatccgtt tacttggtct ggccgatcat atcggaggcg
     4861 ccgatggaaa atgactgttc tgtaaaatct gagcttcctc ctgcagtgca gggtgatgaa
     4921 tttgaatatc cggcactgtt tccgtttgtt gaattgcagg aatttgccgt ggattccaga
     4981 aggaattttc cagtgaagca gctgtagtct atcggagttc cgcccatact cagctgattt
     5041 gcgccgcttt cagagttccc gtaggaagct gtattcaaaa aaatgaatct ggaacaggca
     5101 cctgtttcag agccggtcat agtcattcct gaagcctggc tgtctgccag aagagtgatc
     5161 ataaaagaaa tctgagtcag atcagtgttt gccgctgatg ttattcccag cccgtctgta
     5221 tttactccga tgccgtttaa tgatgatgta tttataaaaa cattgcctga agtttttccc
     5281 cagcggtctg atgccagagc agaattcctg ctgtaatcgc gcatcctcct ctgagggatt
     5341 atttacactc tatcttggca aaagtcagtt tgtttttttt actgtttcct gaagaactcc
     5401 agaaccctga atagttttct ccgtttttgg tcagaataaa tgatccattg gaaccgtcct
     5461 gtttccaggt tccctgtatc ccggtcactc tgccgccgtc attaagccag tcataattca
     5521 tcacaccgtt tttccagtcg tatttgatga tgtaagggtc ctgcttgagt tttcctttga
     5581 ctgtctttcc tgcatttccg gcagccccgc caaaccacgt gatattgaag cattcttcag
     5641 aggaaacagt gacattttgc gccgatatct gcagtatcat tgaacctgcc agtaagatta
     5701 taaagaatct tagtctcatt agtcaatctc cttcggaaga cctatcataa gtatttatat
     5761 attctgtcaa taaataaaat actgctccgg cagtttttta cggattcagc tctgattaag
     5821 cttgactgga tctgcagcgt aaatcagttt tatgaaaaag aagacttgcg gagaaactgc
     5881 ctgaatagac gaatgaaaaa tagttttcat atattatact tttttatatt atttgcagga
     5941 tgttttctca tgcctggaag ggcaagggtt ctcggttttc tgaaagaaat gccttctgaa
     6001 aaaatcagtg agatcagtat tgagccactg gaattccgtt ctcttgtttc ggaacgaatt
     6061 gtgattactg ataaaaa
//
LOCUS       DAOQGJ010000019         5909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_6774_length_5909_cov_3.222924, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000019 DAOQGJ010000000
VERSION     DAOQGJ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6774_length_5909_cov_3.222924"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /gene="rplB"
                     /locus_tag="PK683_00590"
     CDS             complement(<1..413)
                     /gene="rplB"
                     /locus_tag="PK683_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNH06968.1"
                     /translation="MGIRKFKPTTSSLRYKSVLDFAEITAEEPYKPLTKVLNYKAARA
                     TSGRITVRQKGGRVKRKYRVIDFKRTKKDIPALVKTIEYDPNRSAFIALICYKDGEYS
                     YILAPEGLKVGDTVISSDTAEIKPGNTLSLDKIPPG"
     gene            complement(416..724)
                     /gene="rplW"
                     /locus_tag="PK683_00595"
     CDS             complement(416..724)
                     /gene="rplW"
                     /locus_tag="PK683_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002177871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNH06969.1"
                     /translation="MNLDHVIISPLMTEKSQSLKEIGQSIGKRTVKYVLKVHPNANKV
                     LVKDALKKIYNVVPDSVNILVQKGKLKKFRQFPSQKPHWKKAIVTFNNGANIEFAKGA
                     "
     gene            complement(721..1365)
                     /gene="rplD"
                     /locus_tag="PK683_00600"
     CDS             complement(721..1365)
                     /gene="rplD"
                     /locus_tag="PK683_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNH06970.1"
                     /translation="MKARKFSKDGKFLSEVDLPASLFAPKNVSQGAIYDAIKAENANL
                     RQGTHSTKDRGEVRGGGKKPWSQKGTGRARQGSSRAPHWVGGGIIHGPKPRDYSIRLS
                     RNVKRKAVVSILNKKAEEARINILEDLSLNTYSTKTVANVFKNMGLDKTGNIGLVVAG
                     ESKEVKSSVRNIPTVKYINSKRVVCRELLYNNSIVITESALNELVEQYKGVVEQ"
     gene            complement(1389..2009)
                     /gene="rplC"
                     /locus_tag="PK683_00605"
     CDS             complement(1389..2009)
                     /gene="rplC"
                     /locus_tag="PK683_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNH06971.1"
                     /translation="MTKGLIGRKIGMSHVYAENGDVVPVTVLEVGPCSVSQIKTPEKD
                     GYYSVQLAYGDVKEHRISNPEKKHLAKNGISPKRHLKEFKLDGEAPALGTTVTILDVF
                     KELDTVKVTSKSKGKGFQGVTKRHGHHGGPAAHGSRFHRHPGSSGAGTTPARIFKGMK
                     YPGRAGGLQTTVRNLKIVKILKDENLLLVSGSIPGNNKSLVTIEKI"
     gene            complement(2021..2329)
                     /gene="rpsJ"
                     /locus_tag="PK683_00610"
     CDS             complement(2021..2329)
                     /gene="rpsJ"
                     /locus_tag="PK683_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNH06972.1"
                     /translation="MAGQRIRVKLRAFDYKLIDQSTYEIVVTAKRTGAKVSGPVPLPT
                     KVERYTVLRSPHVNKKSREQYEMKTHKRLIDILDTNEDTVEALMKLQLPAGVSVDIKS
                     "
     gene            complement(2335..3549)
                     /gene="tuf"
                     /locus_tag="PK683_00615"
     CDS             complement(2335..3549)
                     /gene="tuf"
                     /locus_tag="PK683_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNH06973.1"
                     /translation="MAKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITSTLAKKIGGKN
                     KSVKYDEIDNAPEEKARGITIATSHQEYETAARHYAHVDCPGHADYVKNMITGAAQMD
                     AAILVVSATDGAMPQTKEHILLARQVGVPYIIVFLNKADMLADDERDDMIAMVEEEIR
                     DLLKKYNFPKEGQGEIPIVHGSALKGLEGEDTELGMKAIMKLMDALDSYVPVPKRVTD
                     KPFIMPVEDVFSITGRGTVATGRVEAGILKVNEEVEIVGIRPTAKTTVTGIEMFRKLL
                     DQAEAGDNIGALLRGTKKEDIERGQVLSKPGSITPHKKFTAEVYVLTKDEGGRHKPFF
                     NNYRPQFYFRTTDVTGVCSLPQGREMVMPGDNVSMTIELISPIAMDKGLNFAIREGGR
                     TIGSGVVAEIIE"
     gene            complement(3574..5451)
                     /locus_tag="PK683_00620"
     CDS             complement(3574..5451)
                     /locus_tag="PK683_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH06974.1"
                     /translation="MIPKTANIGIFAHIDAGKTTFTERILYETGEIPSPGSVEDGTTE
                     SDTLKEEIERGISITSSTVLVRYKHKKEIHYIYIIDTPGHLDFSPQVDSALLAVEFAV
                     ILVDMTAGIRSQTEAVIEKIKARNIPYLIFLNKSDRSMEYEKVLSDVGSLLGRPAVPM
                     FYRADDGTVRYSFLEKEVNEDIELSLIEWDEKLTAKYFSLKDKKKIILQGMSSGFRQN
                     AFVPAFIGSALNGHGVREFLDFLCIVSKDADHPDTGEFIAFKRQNNSHLGQIVYFKSG
                     SRLKPKQTVYRNSEPYTVQEILQIVPGGFQSVQEVFPGSIFVLQDSSFFVGEKLFSEH
                     LESSETEETLIQKDFSILLEPDSDTSRENLEKGIRKLVWEDQGLSCRVKEDTGQLELF
                     GIGELHLEIAVSRLKEIVQENFSIKSINVAKYGLYKNMVKKLCFEHSTFDGKWKSGKV
                     HGFLEARTDLQSYVEFDIPLEGILKDSITSAFSEFTSHGWEGLPVLGMNLRVVGYEAP
                     ELIEEHTPSLIKIAVISGLKSVLPGTVICIGPLSFFEIQVPQNYTGNVISLLNKRHAK
                     VTSLEVADDLKSVLKGEASAEQLLGFTAALRNMTQGKGFVTLNTFFKYDKFSRIAETT
                     D"
     gene            complement(5505..>5909)
                     /locus_tag="PK683_00625"
     CDS             complement(5505..>5909)
                     /locus_tag="PK683_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HNH06975.1"
                     /translation="LYMENMNTLEVYTILKKAKFTQLQSQELANLFGKYYETEPASRR
                     DLNETEVGLKRELKNVESDLKQEIRDVHSEMKLELKNTELRLQKEIEGIRMETQNIRL
                     EIQTVKVDLVKWMIGIVFGAAGLLGAFLKYVK"
BASE COUNT         1471 a         1414 c         1152 g         1872 t
ORIGIN      
        1 ccaggcggta tcttgtctaa tgaaagtgta ttgccaggct tgatttcagc agtgtcgctg
       61 gagatgacag tatcacctac tttcagacct tctggagcaa gaatataaga atactcaccg
      121 tctttgtaac agatcagagc gataaacgct gaacggttcg gatcgtactc aatcgttttc
      181 acaagagcag gaatgtcttt ctttgttctt ttgaaatcga tgactctgta ttttcttttt
      241 acccgtccgc ctttctgacg aactgtaatg cggcctgatg ttgcacgagc tgccttataa
      301 ttcagtactt tcgtaagagg tttataaggt tcttcagcag tgatttcggc aaaatccaga
      361 actgacttgt atctcagtga agacgttgtc ggtttaaact tgcggattcc catatttaag
      421 cccccttggc aaattcaatg tttgccccgt tgttgaatgt tacaattgcc tttttccaat
      481 gaggcttctg agaaggaaac tgtctgaatt tcttcagctt tcccttctga accagaatat
      541 tcactgaatc aggaactaca ttgtatatct tttttaatgc atcttttacc aaaactttgt
      601 ttgcattcgg atgcactttc agaacatatt ttacagttct ttttccaatt gactgcccaa
      661 tctctttgag tgactgagat ttttctgtca tcaatggaga aataattaca tggtccagat
      721 tcattgctca accactccct tatactgctc taccagttca ttgagtgcac tttctgtaat
      781 tacgatactg ttgttataca gaagttcgcg gcacacaact ctctttgaat tgatgtattt
      841 tactgtagga atgtttctca cggaagactt gacttccttg ctttccccag cgactaccag
      901 acctatattt cctgtcttat ccagacccat attcttgaac acattcgcaa cagtttttgt
      961 gctgtatgta ttcagtgaaa gatcttccag gatattaatt ctagcttctt cggctttttt
     1021 gttcaaaatg gagacaacag cttttctctt cacatttctg gaaagacgaa tggaataatc
     1081 tctaggcttt ggcccgtgaa ttataccacc acctacccaa tgaggtgctc ttgaagatcc
     1141 ctgtcttgcc cgaccagttc ccttctgaga ccaaggtttt tttcctccgc ctcgaacttc
     1201 acctctgtcc ttagttgaat gagtcccttg tcttaaattg gcgttttcag ccttgatagc
     1261 atcatatatt gccccctgac tcacattctt gggcgcaaac agcgaagcag gaagatccac
     1321 ttcacttaga aactttccat ctttggaaaa cttgcgggct ttcatctcac gttaccttca
     1381 atattacctt aaattttctc aattgtcact aaggacttgt tattcccagg aatactccca
     1441 gaaactaaga gaaggttctc gtccttaaga atcttgacaa tcttcagatt ccttactgtc
     1501 gtctgaagac ctccggctct tcccggatac ttcatacctt taaaaattct ggcaggagtt
     1561 gttcctgctc cagatgatcc cggatgtctg tgaaatctgg aaccgtgcgc agcaggtcca
     1621 ccgtggtgtc cgtgcctttt tgtcactccc tgaaaacctt tccctttgga cttgcttgtc
     1681 acttttacag tatcaagctc tttgaatacg tccaaaattg taacagttgt acccagtgca
     1741 ggtgcttctc catccagctt aaattcttta agatgtcttt taggagaaat tccattctta
     1801 gcaagatgct tcttttcagg attgctgatc ctgtgttctt tcacatctcc ataagccagc
     1861 tgcacggaat agtatccgtc tttttcagga gtcttaattt gggaaacact gcagggaccg
     1921 acttccaata cagtgacagg tacaacgtct ccgttttctg cataaacatg agacatacct
     1981 atttttcttc caattaatcc tttcgtcatt ttataaaccc ttacgattta atgtccactg
     2041 ataccccggc aggcagttgc agtttcatta aagcttctac tgtgtcttcg tttgtatcca
     2101 aaatatcaat caggcgtttg tgtgttttca tttcatactg ctcacgggac tttttgttca
     2161 cgtgaggtga acgaagaact gtataacgct ccacttttgt aggcagcggg acgggaccgg
     2221 aaactttcgc tccggtcctt tttgcagtca ctacaatctc ataagttgat tgatcaatca
     2281 gcttataatc gaatgctcta agttttactc ttattctttg gccagccatc tcaactactc
     2341 aatgatctct gctactactc cggatccaat ggttcttcca ccttcacgga tagcaaagtt
     2401 taatccctta tccatagcaa tcgggctgat aagctcaatt gtcattgaca cgttgtcacc
     2461 aggcataacc atttctcttc cttgcggcag agaacataca cctgtcacgt ctgtagttct
     2521 gaagtagaac tgtggacgat agttattgaa gaatggcttg tgacgaccgc cttcatcttt
     2581 tgtaagaacg taaacctcag cagtaaactt tttgtgagga gtgatggaac ctggtttaga
     2641 aagaacctga cctctttcga tgtcttcttt cttagtacct ctcaggagtg caccgatgtt
     2701 gtcaccagct tctgcctgat cgagaagttt acggaacatt tcaataccag tcactgtagt
     2761 tttagcagtc ggtctgatac ctacgatttc cacttcttcg ttcactttca ggatacctgc
     2821 ttccactctt ccggtagcaa cagttccacg accagtaata gagaatacgt cttctaccgg
     2881 cataatgaaa ggtttgtcag ttacacgttt tggaactgga acataagaat ccagagcatc
     2941 catgagtttc atgattgctt tcattccgag ttcagtatct tcaccttcga ggcctttcag
     3001 tgcagatccg tgtactattg ggatctcgcc ctgaccttct ttagggaagt tgtatttttt
     3061 caggaggtca cgaatctctt cttcgaccat tgcaatcata tcgtctcttt catcatctgc
     3121 aagcatatct gctttgttca ggaaaacgat gatgtaagga acgcctacct gtcttgcgag
     3181 caggatgtgt tcttttgtct gaggcatagc accgtcagtt gcagaaacca caagaatagc
     3241 cgcatccatc tgagcagcac ctgtgatcat gttcttgaca tagtcagcgt gacctggaca
     3301 atcaacgtga gcatagtgac gcgctgcagt ctcatattcc tggtgagatg tagcaatagt
     3361 tatcccacga gctttttcct cgggagcgtt gtcaatttca tcatacttta cagacttgtt
     3421 tttaccacca atcttctttg cgagtgtgga agtaatcgca gctgttaaag tagtcttacc
     3481 atggtcaacg tgtccaatcg taccgacgtt caaatgcggt ttgcttcgat caaatttttc
     3541 tttagccatt gtcgtttaac tcctcgttaa tcatcaatcc gttgtttcag caattctgct
     3601 gaatttatca tacttaaaaa aagtattcag agtaacaaaa cccttcccct gcgtcatatt
     3661 tcgcaaggct gcagtgaagc ccagcaattg ctcagcagaa gcttctcctt tcaggacaga
     3721 ctttaaatcg tccgccacct caaggctcgt aacttttgcg tgccttttgt tcaggagaga
     3781 aattacattg cccgtataat tctgcgggac ctgaatttca aaaaaactga gtggacctat
     3841 gcagatgact gttccgggaa gaactgactt gagtccgctg atgacagcga tcttgatcag
     3901 gctgggtgtg tgctcctcga taagctcggg ggcttcatat ccaacaactc tcagattcat
     3961 tcccaaaacc gggagacctt cccatccgtg tgaagtgaat tcactgaatg ctgaagtaat
     4021 ggaatcttta agtattcctt ccagaggaat gtcaaactct acataactct gcaaatctgt
     4081 tcgagcctct aagaagccgt ggactttacc actcttccac tttccatcga aagtcgagtg
     4141 ctcgaagcac agttttttta ccatgttttt atatagcccg tacttggcaa catttatact
     4201 tttaatcgaa aagttttctt gtacaatttc tttcagccgg ctgactgcaa tttccagatg
     4261 aagctctcct atcccgaaga gctccagctg acctgtgtct tcttttacac ggcaggaaag
     4321 tccctgatcc tcccagacaa gctttctgat tcccttctcc agattttctc ttgaagtatc
     4381 tgaatccggt tccagaagaa tggaaaagtc tttctggatc agagtctctt ccgtctcgga
     4441 agattccaga tgctccgaaa aaagtttttc accgacaaaa aaagaactgt cctgcagaac
     4501 aaagatactt cccggaaaaa cttcctgtac ggactgaaat cctcccggaa caatctgcag
     4561 aatttcctga acagtatacg gttcagaatt cctgtacacg gtctgcttcg gcttgagcct
     4621 ggagcctgat ttgaaataga caatctgtcc cagatgggaa ttgttctgac gcttgaatgc
     4681 aatgaattcg cctgtatcag gatgatctgc atcctttgat acaatgcaga gaaagtcgag
     4741 aaactctctg accccgtgtc cgttcagtgc acttccgatg aatgcaggaa caaatgcatt
     4801 ttgacggaaa ccggaagaca tcccctgaag aatgattttc tttttgtcct tgagtgaaaa
     4861 atattttgcg gtcagtttct catcccattc gatcagactg agctcaatat cctcgttgac
     4921 ttccttttcc agaaaagagt atcggactgt cccgtcatcc gccctgtaaa acataggcac
     4981 agccggcctt ccgagaagac ttcctacatc cgaaagcacc ttttcatact ccatgcttct
     5041 gtcggacttg ttcaggaaaa tgagatacgg gatattgcgc gccttgatct tttcgatgac
     5101 agcctcagtc tgagaacgga tgcccgcagt catatccaca agaatcactg caaattccac
     5161 cgcaaggagt gcggaatcca cctgaggcga aaaatccaga tgccccggtg tatctataat
     5221 atatatgtaa tgtatttctt ttttatgctt gtaccggaca aggacagttg aagatgtgat
     5281 ggagattccg cgttctatct cctctttgag agtgtccgac tcagtggttc cgtcttccac
     5341 gcttcccgga ctggggatct cgcctgtttc atagagaatt ctttcagtga atgtggtctt
     5401 gcctgcatct atatgggcaa agattccaat atttgcagtc ttcgggatca taaggacagg
     5461 ttttggagat cacctgccta ggtcgagtga aattggacag gagattattt cacgtatttc
     5521 agaaaggcac ccagaagacc ggccgctccg aatacaattc cgatcatcca tttgaccaga
     5581 tcgaccttga ctgtctgtat ttccagacgg atgttctgag tttccatccg aatgccttcg
     5641 atttctttct gaagacgaag ttctgtgttt ttcagttcca atttcatttc tgagtgaaca
     5701 tctctgattt cctgtttcag atctgactcc acatttttca gttccctttt cagaccgact
     5761 tctgtttcat tcagatcgcg cctgctggca ggttctgtct cgtaatactt tccaaaaaga
     5821 tttgccagtt cctgagactg cagctgcgta aactttgcct ttttcagaat tgtgtacact
     5881 tccagtgtgt tcatgttttc catatatag
//
LOCUS       DAOQGJ010000020         5843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_6880_length_5843_cov_3.785418, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000020 DAOQGJ010000000
VERSION     DAOQGJ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6880_length_5843_cov_3.785418"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            601..1428
                     /locus_tag="PK683_00630"
     CDS             601..1428
                     /locus_tag="PK683_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06976.1"
                     /translation="MLENFIKKHKVKLKKVVRVSGILGTGIIFASSFVNDHFVTALQE
                     KTQALDAIQNEKGQAQTAVKSGHDVLEATERLFKIEVTLNSISSKPRKEEFEGLFFTC
                     NSYVDALKGSTEGLKESIADLKGIQKRVTLNDETKKEIEAFSEKIKSLSEKITHEMEP
                     IEKMKADLEKEATAPGSFNFDQGDAALNGVMDKFFLLEDEYKKVGYEIYGLFDKIDEQ
                     VVKEQESSESTLHIVEVLAGFFAFLGAVLAGGGNWLAGRLGMAPGSVPSPEDEEASV"
     gene            complement(1622..2623)
                     /locus_tag="PK683_00635"
     CDS             complement(1622..2623)
                     /locus_tag="PK683_00635"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNH06977.1"
                     /translation="MVDLSTKYMGLNLRTPLVASAGPISYHLDDIKKMEDAGISAIVL
                     YSLFEEQIEKENMELHHHLNHGTHSFAEALTYFPDPGDFESGPDQYLEHIRKAKQSVN
                     IPIIASLNGYTAGGWTKFAEQMQEAGADGLELNLYFVPTDMNVTGKDLEEDFAETLWQ
                     VRKTVSIPIALKLSPYFSNLMNCAQKFSNMGADALVLFNRFYQPDFDLEHLEVKPHVH
                     LSTPHELRLSLRWIALLHSRIKADLAGTGGVHRAEDVLKLLLAGAHSAMLCSVLIREG
                     FGKIKIIENEMLHWLETHEYESVRQMIGSMSAVKYHSPFDYERVQYIKSLHSFHIHH"
     gene            complement(2616..>5843)
                     /gene="nifJ"
                     /locus_tag="PK683_00640"
     CDS             complement(2616..>5843)
                     /gene="nifJ"
                     /locus_tag="PK683_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNH06978.1"
                     /translation="DHSDVMAVRAAGFALLSSDSVQCAHDFAVISHAATLESRIPFMH
                     FFDGFRTSHEVSKIEMIDDDTLRKIIREDLIAEHRKRALNPDHPVLRGTAQNPDVYFQ
                     GRETVNPYYAKCPETVEKLFIEFEKITGRKYKLFEYFGAPDAERLIVLMGSGAETVHE
                     TVEYLLSKGEKVGVLKVRLYRPFSAERFLQEIPSTVSSIAVLDRTKEPGSSGEPLYLD
                     TVEAFFKDFVSGSKKWKTLPKIVGGRYGLSSKEFTPAMVRSVYDELKKEKPKNGFTVG
                     IHDDVMNTSLDFDPDFSIEKPETIRCMFYGLGSDGTVGANKNSIKIIGENTDHYVQGY
                     FVYDSKKSGSMTISHLRFGKSQIRSSYLINHPNFVACHQSIFLDRFDMLAGIETGGTF
                     LLNTVYGPDQIWSKLPGQIQKDIIEKKLKFYVIDGYKAAASAGMKNQINTVMQTCFFS
                     ISGVLPKEEALEAIKEAIRKTYGKKGGEIVNRNIQAVDDTVSHLYQVDCSQIKTDVFN
                     DIHEPGIKISGKAPEYVQKVLGKMLAAKGDMLPVSAMPADGTFPTGTSKWEKRKIAQE
                     VPVWDKNVCIQCGKCSYVCPHSVIRMNVFKEDALKNAPSGFETSDSRIPELKEQGYKY
                     TLQISEDDCTGCGICVDICPAKNKSEVRLKAINLKPVDSIREGRKEEWDFFLSIPPVD
                     RNLLNIHQIRQQQLQEPLFEFSGACSGCGETPYVKLVSQLFGDRMVVANATGCSSIYG
                     GNLPTTPWTKNGAGRGPAWSNSLFEDNAEFGYGFRVSIDKQRESAMELVLELKNQIGA
                     ELAESILNSDQTEEAEIFEQRKRVEKLNNILEKLKKDTQYSLRAEKLLQISDKLVKKS
                     VWIIGGDGWGYDIGFGGLDHVLASGRNINVLLLDTEVYSNTGGQMSKSTPRAAVAKFA
                     SGGKPASKKDLGLIAMSYGNVYVATVAMGAKDEHTLRAIMEAERYDGPSIIIAYSHCI
                     AHGINMTEGMKHQKAAVETGQWILYRYNPDRASQGMNPLEIDSAPPHVPVRNYFESEN
                     RFKMLTKSRPEAAKELFRQAQEDIQKRYSYFEFLSGKKKEEDHHG"
BASE COUNT         1565 a         1311 c         1192 g         1775 t
ORIGIN      
        1 aaattcacct gtccagcctt acggtcaatg ttctcaagtc aaggccagtg tcgcctgttg
       61 aactataacc tttctaattc aatccttcga cggactaaag gtatgacaaa tcattgctaa
      121 tcgtacagaa ttgaccgacg atatcgtctt taatgcctgc ctgctcagct gttaaggata
      181 tccgctgttc taaaaaacat atactgatcg ggatgatcgg cctacttgca gcggcttccg
      241 gtaatactgt tgtttgccgt ccgtggaaaa tgaagctgtc aatcgcttgg cagtctttaa
      301 gaccttgttt tcaagcttcc attgtaattt ttactgagca gctgcggata attttcgcat
      361 ttctcgaaac agtgtctgaa tgattggaga aatgactctt gattttccgg tcagattctt
      421 caataataga aagcagtaaa acctgcactt tcctcaattt ttttcttgaa aaaatcagtc
      481 cctatttata ctgtcctgac agtcttttat ttttaaattc agttttttta tagtaaagga
      541 cgatactgtt cttttgattc agaagagcag acagaaactt tagaaagaca ggagcagata
      601 atgctggaaa attttataaa aaaacataaa gtaaagctga aaaaagttgt ccgtgtttca
      661 ggaattcttg gaacaggcat tatttttgcg tcatcattcg taaatgatca ctttgtgaca
      721 gcacttcagg aaaagactca ggctcttgat gcaattcaga atgaaaaagg acaggctcag
      781 acagcagtca agagcgggca tgatgtcctt gaagcgacag aaaggctttt taaaattgaa
      841 gtcacactaa attcaatatc cagcaaaccg agaaaagagg aatttgaagg tctgttcttt
      901 acctgcaatt catatgtgga tgctctgaag ggaagtactg aaggattaaa agagagtatt
      961 gctgatttaa aaggaattca gaaaagagta acgttgaatg atgaaacaaa aaaagagatt
     1021 gaagcattca gcgaaaaaat aaaatctctg tctgaaaaaa taactcacga aatggaacct
     1081 attgaaaaaa tgaaagctga tttggagaaa gaagccactg ctcccggttc attcaatttt
     1141 gatcagggtg atgctgcatt gaacggggtc atggacaaat tctttcttct ggaagatgaa
     1201 tataaaaaag tcggctatga aatttatgga ctctttgaca agattgacga gcaggttgtg
     1261 aaagaacagg aaagcagtga atccactctg catatagtgg aagtgcttgc cggtttcttt
     1321 gcgtttctcg gtgcggtgct tgcgggtgga ggaaactggc ttgcaggccg attgggaatg
     1381 gctccaggct cagttccttc acctgaagat gaggaagctt cagtctaaaa aaacagtcat
     1441 aaactgtaat cctcagcttg tatgggaagc ataacggatc tgttctcccc cgcagagtaa
     1501 aacgtcagct gaagatacag ggcggcaata tcggaattat actccttttt ggccagtcag
     1561 cagagaggac gcctgaacag catcataaat aaaatatgct tccaggagtc ctcattattt
     1621 tttaatgatg aatatgaaaa ctgtgcagag actttatgta ctgcactctt tcataatcaa
     1681 acggtgaatg atatttcact gcactcatac ttccgatcat ctgtctgacg gattcatatt
     1741 cgtgagtctc cagccagtgg agcatttcat tttcaatgat ctttattttt ccgaatcctt
     1801 cacggatcag cacagaacag agcatggctg aatgagctcc ggccagaaga agtttgagta
     1861 catcttctgc cctgtgcact ccccctgttc ctgcaagatc tgctttgatc ctggaatgaa
     1921 gaagtgcaat ccatctcagt gaaagtctaa gctcatgagg agtggataag tgcacatgcg
     1981 gtttaacttc cagatgttcc agatcaaagt ccggctgata aaacctgttg aagagcacaa
     2041 gtgcatcagc tcccatattc gaaaacttct gagcacagtt catcagattg ctgaaatagg
     2101 gacttaattt caaagcaata ggtatgctga cagtctttct gacctgccag agagtctctg
     2161 caaaatcctc ttccagatcc tttccggtca cattcatatc tgtaggtacg aaatacagat
     2221 tcagttccag accgtcggcc cctgcttcct gcatctgttc tgcaaatttt gtccagcctc
     2281 ctgcagtata accgttgagg cttgcaataa tagggatatt tactgactgt tttgctttcc
     2341 ggatatgctc cagatactga tcagggcctg attcaaaatc gccaggatca ggaaaataag
     2401 taagagcttc tgcaaaactg tgagttccgt gattcagatg gtgatgcagt tccatatttt
     2461 ctttttctat ctgttcttca aaaagagaat acagcacaat tgcagagatt cctgcatctt
     2521 ccattttttt aatatcatcc agatgataag aaatcggacc tgcagacgca acaagcggcg
     2581 tccttaaatt cagtcccata tattttgtgg ataaatcaac catgatgatc ttcctccttc
     2641 ttttttccgg aaagaaattc aaaataactg tatctcttct gaatgtcttc ctgagcctgt
     2701 ctaaagagct cttttgcagc ttccggtctg ctctttgtca gcattttaaa tctgttctct
     2761 gattcaaagt agtttcttac agggacgtga ggaggtgcag aatcaatttc aagaggattc
     2821 attccctgtg aagccctgtc gggattgtat ctgtagagta tccactgacc agtctccact
     2881 gcggctttct gatgtttcat tccctcagtc atattgattc catgcgcaat acaatgactg
     2941 tatgctataa tgatggaagg tccgtcatat ctttctgctt ccatgatggc gcgaagagtg
     3001 tgctcatcct ttgctcccat tgcaactgtc gctacatata catttccgta gctcattgca
     3061 atcagcccca gatccttctt cgaagcaggt ttgcctcctg aggcaaattt tgcaactgca
     3121 gccctgggcg tggacttgga catctgcccg cctgtattgg agtagacctc cgtgtccaga
     3181 agaaggacat tgatgtttct tccgctcgca agcacatgat ccagacctcc gaatccgata
     3241 tcatatcccc atccgtctcc gccgatgatc cagacacttt tcttgaccag tttgtcggaa
     3301 atctgaagaa gtttttccgc cctcaaggaa tactgtgtat ccttttttaa tttttctaga
     3361 atattattca gtttttcaac tctttttctc tgttcaaaaa tttctgcttc ttctgtctga
     3421 tccgagttca gaatggattc agccagttcc gctccaatct gatttttgag ttccagtacc
     3481 agttccattg cagattctct ctgcttgtca atggaaaccc tgaaaccgta tccaaattct
     3541 gcgttgtctt caaagagtga atttgaccag gcgggccccc tgcctgcacc gttctttgtc
     3601 cagggtgtcg taggaaggtt tccgccgtaa atggaagaac atcctgtcgc atttgccact
     3661 accatcctgt caccgaaaag ctgcgatacc agttttacat acggtgtttc tccgcatcct
     3721 gaacatgcgc cggaaaactc aaacagaggt tcctgaagct gctgctgacg gatctggtga
     3781 atgttcagaa gattcctgtc tacaggaggg atggataaaa agaagtccca ttcttctttt
     3841 ctgccctcac gtatgctgtc caccggtttc agattgattg ccttcagtct cacttccgat
     3901 ttgttttttg caggacagat gtccacacag attccgcatc cagtgcagtc gtcttccgaa
     3961 atttgaagag tgtacttgta tccctgttcc ttcagttccg gtattctgga atcggaagtt
     4021 tcaaaaccgg agggagcgtt tttcagtgca tcttccttga atacattcat ccgaatcact
     4081 gaatgaggac atacatagga gcatttaccg cattgtatgc atacattttt gtcccagacc
     4141 ggaacttcct gagcgatttt tcttttttcc catttcgatg tgcctgtagg aaatgttccg
     4201 tccgcaggca ttgcactgac tggaagcata tctcctttcg ctgccagcat ttttcccaga
     4261 actttctgaa catactcagg tgctttgccg gagattttaa ttccaggttc gtgaatatca
     4321 ttaaagacgt cggtcttgat ctgactgcag tctacctgat acagatggga tacggtatca
     4381 tccaccgcct gaatattcct gtttacgatt tctccgcctt tctttccata cgttttacga
     4441 atggcttcct tgatcgcctc aagagcttct tctttcggaa gaactcctga gatgctgaaa
     4501 aaacaggtct gcattactgt attgatctga tttttcattc ctgcggatgc ggcagccttg
     4561 tatccgtcaa tgacgtaaaa cttcagcttc ttttcaatga tgtccttctg tatctgtccc
     4621 ggaagttttg accagatctg atccggaccg tatactgtgt tcagaaggaa tgtccctcca
     4681 gtctcaatcc ctgcaagcat atcaaaccgg tccagaaata tggactgatg acaggcaaca
     4741 aaattcggat gattgatcag atagctcgaa cggatctgag attttccgaa acgcagatgg
     4801 gaaattgtca tcgatccgga ttttttggaa tcatatacaa aatatccctg aacataatga
     4861 tccgtatttt ctccgatgat tttgatggag ttcttgtttg ctccgactgt tccgtctgat
     4921 ccgagtccgt aaaacataca cctgattgtt tccggctttt caatgctgaa gtccggatca
     4981 aaatccagac tagtattcat tacatcatca tgaattccca ctgtaaaccc gttctttggt
     5041 ttttctttct tcagttcatc atataccgac ctgaccattg cgggggtaaa ttccttggag
     5101 gaaagtccgt atcttccgcc gacaatttta gggagtgttt tccatttttt gcttccggaa
     5161 acaaagtctt taaaaaaagc ctctactgta tcgagataaa gaggttcacc ggaacttccc
     5221 ggttcctttg ttctgtccag aactgcaatg gaactgactg tagaaggaat ttcctgaaga
     5281 aatctttctg cgctgaaagg tctgtacagc cttactttca gaactccgac tttttctcct
     5341 ttagaaagaa gatattccac tgtttcatgg actgtctccg cacctgaacc catcagaaca
     5401 atgagtcttt cagcgtcggg cgcgccaaaa tactcaaata gtttatattt tcttccagtg
     5461 attttctcaa actctataaa cagcttttct actgtttccg ggcattttgc atagtatgga
     5521 ttgactgttt ctcttccctg aaaatacaca tcagggttct gagcagttcc cctcagaacc
     5581 ggatgatccg gattcagtgc tcttttcctg tgctctgcaa tcagatcctc gcggatgatt
     5641 ttccgcagag tatcatcatc aatcatttcg attttcgaaa cctcatgaga ggttctgaat
     5701 ccgtcaaaaa aatgcataaa cgggattctg ctttccagcg tcgctgcatg agatatgaca
     5761 gcaaaatcat gtgcacactg aactgaatcg gaagaaagca gtgcaaaccc tgctgctctg
     5821 actgccatca catctgagtg atc
//
LOCUS       DAOQGJ010000021         5779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_6993_length_5779_cov_3.623864, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000021 DAOQGJ010000000
VERSION     DAOQGJ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6993_length_5779_cov_3.623864"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1520
                     /locus_tag="PK683_00645"
     CDS             210..1520
                     /locus_tag="PK683_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNH06979.1"
                     /translation="MREIKTVTILGANGAMGAGSAGVIASFGEAKVYMLARDLGKANE
                     GIEKAIQSVRTDTIRSRMIAGTYDNDLAKAVADSDWVFELVAESYDVKEAINAQIAKH
                     KKPGTIISTVSSGLSIERLSKAFDTDGQKHYFGTHFFNPPYKMILCELVSHKGSDPKI
                     KKQLGKYLEEVLGRAVVYTNDTPAFAGNRIGFQLMNEIAQVAEKYSDKGGLALMDEIM
                     GGFTGRAMGPLATVDFVGLDVHKAIVDNLYQFTKDGAHETFKLPGYMQKLIDSGNLGA
                     KSGQGLFKRTKTADGKREKFVYNISTGNYDPYPKFDIPFTKEAGKKIAASDYAGAMDV
                     IKNAKGFEAELCRYFIARYISYSLSIVGEVVENKENADAAMGFGFNWVPASAFVDFLG
                     GVTESIKFLEQSKIEVPAVLKNADRTKPFYQLKDKLDARALFRG"
     gene            1533..2798
                     /locus_tag="PK683_00650"
     CDS             1533..2798
                     /locus_tag="PK683_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNH06980.1"
                     /translation="MSDKVYVLGGEQTDFQRNWSKEGKTFMAMMKEVIEDALVKVGID
                     YDELKRLNKKNRIGVFVGNFDAEQYANQGHLGAFLTEVNPAFYGIPGARYEAACASGS
                     VALDAAHAHIRAKDYDVAIVLGIEVMKTVSSAVGGDFLGTAAYYEREAKGVEFPFPKL
                     FGRLADVILERYKLKEEVFMDALAEISRINYANAKRNPKSQTRTWFMNKEHASARGGA
                     NNMSVGGRLCITDCSQVTDGAAVVVLASKSYAKEYAKARNIKTSKIPHIKGFGHRVAP
                     ITFTGKVAESEGNKFILPWTKIAVDDAYGRAGLSVKDIDVFETHDCFTSSEYAAVSAF
                     GISEPGKEHEAIQSGVIDFTGSKPINPSGGLIGVGHPVGASGVRMMLDLYKQVTGQAG
                     GYQVKGAKNGLMLNIGGSATTNMVFILGR"
     gene            2878..3873
                     /locus_tag="PK683_00655"
     CDS             2878..3873
                     /locus_tag="PK683_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06981.1"
                     /translation="MYLPRHVKLETRKQTKVLPVILLIIFITAVFFSIYKRKQIMLFF
                     KGSSAEKHTKAENRLIESVLQGEKNKDYLQEYISNVQALLHTEPVNSRGYFAQARVHY
                     LEALFSGFELKTIHLIEKLSRTGTVPDKILDDFSVHLEPMYRQALRARAFSAEFGDSN
                     SNLILVLLYEALQNRKNFSLIAGELKTVQYERVSPELRKNYIWLSLLSSVKSADFDFL
                     NQLLKMNKDLQGKSNLEITEREESYLQGLTFFYKKDFIRSLELIRKVQKEQDRLGIEA
                     GKTEAEIFFLQNLHEKAIVILENLFEVSGKQDKDIQKRLQKIVESKSGLKSKYLK"
     gene            3946..4956
                     /locus_tag="PK683_00660"
     CDS             3946..4956
                     /locus_tag="PK683_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH06982.1"
                     /translation="MKIISLLALFLLHCSLHSYKLNGEEIRTKRVAPNELSVNYDIHR
                     KTPANENDELTRGYNKESLYNIIERSCCIRRSPNSNIIMKYETDVKGELRYPLTVFFW
                     MATLGIIPVFEEAIATVTVTLEKKDTGEIINQFTYRIDEYVIHSWLTIPFGLTLSGSE
                     SHSLSFLHSEYPMEFITDRLAYDLLRGGSRPALTENKTPPSLTPASSKVAVLPFNYKF
                     KKHEHLSETLRDKVETLLVNKKYTVLDRMNLKQILKEMKLSQTGLTIADQIKIGKMMN
                     ATNLITGEILETNDKDDISEFSVKNIDIQTGKIIWKHEFQISENSSSSLNSAIQELGN
                     RI"
     gene            complement(4959..5573)
                     /locus_tag="PK683_00665"
     CDS             complement(4959..5573)
                     /locus_tag="PK683_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06983.1"
                     /translation="MDLFIRIKYKVLDFLGGLNQGLIVTHPLRIFLFFILISPILIPI
                     ALGGLFGLLVCIFAPVSLIKTAGSFDMTHFPDLIKMLMIWIFGVLICGTSFIMGTAGI
                     LVYLADMYWTVYLSIYGKMVKGRIVSFWKTSSRAGNSTTHSAHCTVSFVSDDSRQEIR
                     LSGASQRSFLQEILEIEIEKERDVMIVYSEKHPSIARLKHTDNI"
BASE COUNT         1898 a         1165 c         1260 g         1456 t
ORIGIN      
        1 ggcaagccaa ttcccacgcg aggaaacggg aattgccttt ctggaaagac agctcaaaaa
       61 aaggagttcc cacaattcga caggataaat gagacataac cctcaggatt cggccggaat
      121 acgggcgata aaaaagaaaa acagctttcc tcctgaacgc tgtttttaaa actaaactta
      181 aagagtaaaa tttcattcag gtcgaagata tgagggaaat aaaaactgtt acaattcttg
      241 gtgctaacgg ggccatggga gctggaagcg ctggtgtgat tgcttctttt ggggaagcaa
      301 aggtttatat gctggcaagg gaccttggaa aggccaacga ggggattgaa aaagcaattc
      361 agtcagttcg gaccgataca attcgttcca gaatgatagc gggaacctac gacaatgatc
      421 tggcaaaagc agttgccgat tcagactggg ttttcgaact cgttgcagaa agttacgatg
      481 taaaagaagc aatcaatgca cagattgcaa aacacaaaaa gcctggaaca atcatttcca
      541 ctgtgtcttc cgggctttcc attgaaagac tgtccaaggc atttgataca gacggtcaga
      601 aacactattt cgggactcat tttttcaatc ctccttacaa aatgatcctc tgtgaacttg
      661 tttctcataa aggatctgat ccaaaaatca aaaagcagct cggaaaatac ctggaagaag
      721 ttctgggaag agcagttgta tatacaaacg atactcctgc atttgcagga aacagaatcg
      781 gattccagct gatgaacgag attgctcagg ttgcagaaaa atactccgac aagggcggac
      841 ttgctctgat ggatgaaatc atgggcgggt ttacaggacg cgcgatgggt cctctcgcca
      901 ctgtggattt cgtcggtctg gatgttcaca aggcgattgt agacaacctc tatcagttta
      961 caaaagacgg tgcacacgaa actttcaaac tccccggtta tatgcagaaa ctgattgatt
     1021 caggaaatct gggtgcaaag tcgggtcagg gacttttcaa aagaaccaag actgctgacg
     1081 gaaaaagaga gaagtttgta tacaatatat ccaccggtaa ttatgatccg tatccgaaat
     1141 tcgacatccc ctttacaaag gaagcaggga aaaaaatcgc tgcatccgat tatgcaggag
     1201 caatggatgt aattaagaat gcaaaaggat ttgaagcaga actctgcaga tatttcattg
     1261 caagatacat cagctattca ctttcgattg tgggtgaggt tgtcgagaac aaggaaaatg
     1321 cggatgccgc aatgggattc gggttcaact gggttcctgc atctgctttt gtagatttcc
     1381 tcggcggagt cactgaatcc atcaagttcc tggaacagtc aaagatcgaa gttcctgctg
     1441 ttctgaaaaa tgcagacaga acaaaaccgt tctatcaatt gaaagacaaa ctggacgcaa
     1501 gagcgctctt cagaggctga taaggaggaa acatgtcaga taaggtttac gtattaggcg
     1561 gggaacagac agattttcag agaaactggt ccaaagaagg caagactttc atggcaatga
     1621 tgaaagaagt catcgaagat gctcttgtaa aagtcggtat cgactatgat gaactgaaaa
     1681 gactgaacaa gaaaaacaga atcggcgttt tcgtaggaaa ctttgatgct gagcagtatg
     1741 caaaccaggg acatctggga gcattcctca ctgaagtgaa tcctgcattc tacggaatcc
     1801 cgggtgctcg ttatgaagca gcatgtgctt ccggttcagt tgcactggat gcagctcacg
     1861 ctcatatccg tgcaaaagac tatgatgtgg caatcgttct cggaatcgaa gtgatgaaaa
     1921 cagtcagctc tgcagtcgga ggagatttcc tcggaacagc tgcatactat gaaagagaag
     1981 caaagggagt ggaatttcct ttccctaaac ttttcggaag acttgcggat gtcattctgg
     2041 aaagatacaa gctgaaagaa gaagttttca tggatgcact tgctgaaata tccagaatca
     2101 attatgcaaa tgcaaaaaga aatcctaagt ctcagacacg tacatggttt atgaacaagg
     2161 aacatgcaag tgcaagaggc ggagcgaaca atatgtctgt cggaggaaga ctctgcatta
     2221 ctgactgttc acaggtcact gacggagcag cagttgttgt tctggcatcc aagtcctatg
     2281 caaaggaata tgcgaaagca agaaacatca agacttccaa gattcctcat atcaaaggat
     2341 tcggacacag agttgctcca atcactttca ctggaaaagt ggccgagtct gaaggaaaca
     2401 agttcattct gccttggaca aaaatcgctg tggatgacgc gtatggaaga gcaggtcttt
     2461 ccgtaaaaga cattgatgtg tttgaaactc acgactgttt tacttccagt gaatatgctg
     2521 cagtatcagc attcggaatt tccgaaccgg gaaaagaaca tgaggcaatc cagagcggag
     2581 tgattgattt tacaggaagc aaaccgatca atccgagcgg aggactcatc ggagtcggtc
     2641 acccagtcgg tgcaagcggg gtcagaatga tgctcgatct gtacaaacag gtaaccggtc
     2701 aggcaggagg atatcaggta aaaggtgcga aaaacggcct tatgctgaat atcggcggat
     2761 ctgctacaac aaatatggtt ttcatcctcg gacgataaac cgaccaatcg atttcagatc
     2821 tagaaccaga aattttagat ctgaaatcat aaatccacag tcaagtataa cccttaaatg
     2881 tacctaccca gacacgtaaa attagaaacc agaaaacaga ctaaagttct gccagtcatt
     2941 cttctcatta tttttatcac tgctgtattt ttttctatat ataaaagaaa acagatcatg
     3001 cttttcttca agggcagttc cgcagaaaaa cacactaaag cggaaaacag actgattgaa
     3061 tcagttctgc agggagaaaa aaacaaggat tatctccaag agtatatatc caatgtgcag
     3121 gcacttcttc atacagaacc tgtcaacagc agaggatatt ttgctcaggc gagggttcat
     3181 tatctggaag ccttgttttc cggatttgaa ttaaagacaa ttcatctgat cgaaaaactt
     3241 tccagaacag gaacagttcc ggataaaata ctggatgatt tttccgttca ccttgaaccg
     3301 atgtacagac aggcacttcg tgcaagggca ttctccgcag aattcggaga ttcaaattcc
     3361 aatctgattt tagtgcttct gtatgaagcc cttcagaaca ggaaaaactt ttctctgatt
     3421 gcaggggaac tcaagacggt acagtacgaa agagtttctc ctgaactcag aaaaaattat
     3481 atctggctca gtctgctgtc ttcagtgaaa tctgctgact ttgacttcct gaatcagctt
     3541 ctgaaaatga acaaggatct tcagggaaag tccaatctgg agatcacaga aagagaagag
     3601 tcctatcttc agggactcac attcttctac aagaaagatt tcattcgttc tttggaactt
     3661 atccgaaagg ttcagaagga acaggacagg cttggaattg aagcaggaaa gacggaagcg
     3721 gaaatattct ttcttcagaa tcttcatgag aaggcaattg tcattctgga aaatctgttt
     3781 gaagtttcag gaaaacagga caaggatatt cagaaaagac tgcagaaaat tgtagaatcc
     3841 aaatcaggac tgaaatcgaa gtatctgaag taaacacaat tgcttctctg ctgaaagtca
     3901 ttttttattt ctcgacaatt tcctttcctg tttaaaatgc agaatatgaa aataatctct
     3961 cttcttgcac tgttcctcct ccactgctct ctgcacagtt acaaactgaa cggggaggaa
     4021 atcaggacca aaagagtggc accgaatgaa ctttccgtaa actacgatat tcacaggaag
     4081 acccctgcaa acgaaaatga tgaactgaca agaggttaca ataaggaatc attatataat
     4141 atcatagaga gatcctgctg catacgcaga tccccgaact caaatatcat catgaagtac
     4201 gaaacagatg taaaaggtga actgagatac ccgctgactg tctttttttg gatggcgact
     4261 cttggaatta ttccggtatt tgaagaagcg atcgcgacag ttacagtgac actggaaaaa
     4321 aaagatacag gcgagataat aaatcagttt acttatagaa ttgatgaata tgtaattcat
     4381 tcctggctga caattccgtt cgggctgact ctgtccggaa gtgaatcaca cagcctcagt
     4441 ttccttcatt cagaatatcc gatggaattt attacggaca ggcttgcata tgatctgttg
     4501 agaggcggaa gcagacctgc tctgacagaa aacaaaacac ctccttctct cactccagcc
     4561 tcttcaaagg ttgcagttct tccgttcaat tataaattca aaaaacatga acacctctct
     4621 gaaacactca gggacaaggt ggaaactctt cttgtaaaca agaaatacac tgttcttgac
     4681 aggatgaatc tgaagcagat cctgaaggag atgaaacttt ctcagacagg actcactatt
     4741 gccgatcaga tcaaaatcgg aaagatgatg aatgcaacaa acctgatcac aggcgagatc
     4801 ctggaaacaa acgacaaaga cgatatttca gaattttcgg taaagaacat tgatattcag
     4861 acaggcaaga tcatctggaa acatgaattt cagatttccg aaaacagttc ctcttcattg
     4921 aattcagcaa tacaggaact gggaaacagg atataaaatt aaatattgtc agtgtgcttg
     4981 agtctggcaa tggaaggatg tttttctgaa tatacaatca ttacatccct ttctttttca
     5041 atttcgatct ccaggatttc ctgcagaaaa gatctttgac ttgctcctga aagtctgatt
     5101 tcctgcctgg aatcatctga aacaaaagaa acggtgcagt gagcactgtg agttgtgctg
     5161 tttcctgccc tggaactggt cttccagaag gatacaatcc gtcctttcac catttttccg
     5221 taaatagaaa gatataccgt ccagtacata tctgcaagat acacaagaat gcctgcagtc
     5281 cccataatga aactcgtccc gcagatcaga actccaaaaa tccagatcat aagcatcttt
     5341 attaaatcgg gaaaatgggt catatcaaaa gaccccgcag tttttatcag agatacaggt
     5401 gcaaaaatac agacaagcag accgaaaaga cccccaagag caatcggaat caggatcggg
     5461 ctgatcagaa taaagaaaag aaaaatacgg agcggatgag tcacaatcag tccctgattc
     5521 agacctccta gaaaatccaa aactttatat tttatgcgaa taaacagatc catatgagtc
     5581 tcctttttta ttttaatgca attataatta aggaaaaata atacttatat ttcctgtaat
     5641 gtttccgtga atgactgcaa ttccaccgtt ataaattcct gagccgatat tgaatgaatt
     5701 tccgccagtc gcgactatgc ttccaagcgt gatcccggca gaaaatgttc cagtggaagg
     5761 gtcaaaaatt gatgtggcc
//
LOCUS       DAOQGJ010000022         5727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_7077_length_5727_cov_2.782087, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000022 DAOQGJ010000000
VERSION     DAOQGJ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7077_length_5727_cov_2.782087"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PK683_00670"
     CDS             <1..677
                     /locus_tag="PK683_00670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388290.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNH06984.1"
                     /translation="FQSEVKQFLKGFKATLEEFNSLLMRNKIFNERTDGVGGISAEDA
                     LDYGYSGPNLRAAGVPHDVRKDSPYMFYDKVDFDIPIGEDGSVLHRALVRMEEMRQSV
                     RIIEQLIDNIPAGPFHADLPHAFLPDKQKVYSSMEELIYHFKIIMHGVKVPPGEHYFA
                     TEAANGELGFYIVSEGDKSPWRVHVRRPCFWYYQSFPQLVKGSLIADSIATMSSLNVI
                     AGELDC"
     gene            679..1158
                     /locus_tag="PK683_00675"
     CDS             679..1158
                     /locus_tag="PK683_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002746499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNH06985.1"
                     /translation="MAYQFSQESEGKFQKLIPMFPDKRSLILPALYLLQKDQGFVDTV
                     GMQYIADRIGAPIVLSHVYGVATFYTLYNKKPVGKYHIQVCANISCCVMGSDRITNTV
                     CKKLGIKKGETTPDKKFTVDEVQCLGACGYGPMAQINDQYYEFLDEKKITEILDSLE"
     gene            1162..2415
                     /gene="nuoF"
                     /locus_tag="PK683_00680"
     CDS             1162..2415
                     /gene="nuoF"
                     /locus_tag="PK683_00680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNH06986.1"
                     /translation="MAELKILTKHRGEKDTHTISHYQSVGGYTAVKKALSMQGDDIVA
                     TVKNSGLRGRGGAGFPTGMKWSFIPKNINKPKYLLCNGDEGEPGTFKDRVLLEEFPHA
                     MIEGMIIAAKAIDSHQGYIYIRGEYHKSISRVEQAINEAYAAGLLGKNIQGSGFDFEL
                     CVYQGAGAYICGEETALINSLEGRRGHPRLKPPFPAVSGLYGCPTVVNNVETFSAVPH
                     IINNGAEWYAKIGTPKSTGTRLFSVSGPVKKPGVYEIPLGTPLMTLINDLCGGMADGK
                     KLRGIIPGGSSVPILTAEECKTANMDFESMAEHKTMLGSGAVIVLAEDTDIVETTFRL
                     AEFYAHESCGQCTPCREGTHWVADLLHKIRDGHGTKKDLELILSLTVNMEGGTTICPL
                     ADACVGAVRPTIQKYRPEFEARLKG"
     gene            2618..3688
                     /gene="nuoH"
                     /locus_tag="PK683_00685"
     CDS             2618..3688
                     /gene="nuoH"
                     /locus_tag="PK683_00685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNH06987.1"
                     /translation="MDWNLVLVWALKSGILFFIIITACAYYTLAERRVCGWIQDRPGP
                     NRAGPFGLFQPLADGVKFLTKEEVFPTNVNKGLYLIAPAISVSCAIMAWSIIPFGGTI
                     PLPADLAKAVGFDKLDLQVANPDTGILFLLAISSLAVYGIILAGWSSNNKYSLMGGIR
                     STAQMISYELPLGLALTSIILLSGSLRLTDINDAQKGMWFIFTLPGFIAFIIFSVAMF
                     AETNRLPFDLAEAESELVVGFHTEYGAFKFALFFIAEYMNMLTMSCVVTLLFFGGYNL
                     PLGILKGSQFEALAGLGFFAVKVIFFAFCFIWVRWTLPRFRYDQLMNIGWKKLIPWAL
                     ANIVLAGTYVVYWGKYWKGIFN"
     gene            3693..4289
                     /locus_tag="PK683_00690"
     CDS             3693..4289
                     /locus_tag="PK683_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002746377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNH06988.1"
                     /translation="MLDLNHPQSLFFLIFAGITVGSALAVVLHKNAVVSAASLVLSFF
                     ALAGIYAVMNAVFIATMQVLVYAGAIMVLVIFVIMLLSLREETGKEIFRKPIKQTFIF
                     LLVVVFGALLITASNYAPDVGRNSADRVKAVPYSYTLKQDAKAPVQSKGNTATVGAST
                     FIDYLLPFELVSILLLAAVVGAVLLAKREKIKNQGGAL"
     gene            4286..4606
                     /gene="nuoK"
                     /locus_tag="PK683_00695"
     CDS             4286..4606
                     /gene="nuoK"
                     /locus_tag="PK683_00695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNH06989.1"
                     /translation="MNHLISGIPLGYFLSLAGILFSIGVFGVLFRRNAVVIFMSVELM
                     LNSVNLVFISFSKAMSQVSGEVIVFFVMAIAAAEAAIGLAIVIAIHRHKKTVYVDEIN
                     LLKW"
     gene            4611..>5727
                     /locus_tag="PK683_00700"
     CDS             4611..>5727
                     /locus_tag="PK683_00700"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNH06990.1"
                     /translation="MLDLFPLVVLLPLAGFLINGFFHRKIPHTAAAVIGISAVAVPFF
                     ITLGALFEFQPLVRKTPHLFSILSWIHAGKFAADFAYQVDQLSLYMTLIITGIGSLIH
                     LYSVGYMHGESGFNRFFVYLNLFIFSMLNLVLGDNLVLMFLGWEGVGVCSYLLIGFDF
                     HKSSAANAGMKAFIVNRIGDVGFAVGIFLTYWYLGSVKYVDIMTALPTALPFKEIINW
                     VALAFFIGAIGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVFMIARLNPIFYA
                     AEHASTYIAFTGAVTALFAATIGLFQTDIKKVLAYSTVSQLGYMFLAMGVGAYTAGMF
                     HLMTHAFFKALMFLGSGSVIHAMHHEQDMRNMGNLKNH"
BASE COUNT         1507 a         1166 c         1378 g         1676 t
ORIGIN      
        1 aatttcagtc ggaagtgaaa cagttcctca agggcttcaa ggctacgctt gaggaattca
       61 acagtcttct gatgagaaat aagattttca acgaaagaac agacggggtc ggcggtattt
      121 ctgcagagga tgctctggat tacggttatt ccggaccgaa tctccgtgca gcaggcgttc
      181 cgcacgatgt gagaaaagac agtccctata tgttctatga caaagtggat tttgatatcc
      241 ctataggaga ggacggatct gttctgcata gagcacttgt gagaatggaa gaaatgcgtc
      301 agtctgtgag aatcatcgaa cagctgatcg acaatattcc tgcaggaccg ttccatgcag
      361 atcttcctca cgcattcctt ccggacaaac agaaggtcta cagcagtatg gaagaattaa
      421 tttatcattt taaaatcatt atgcatggag taaaggttcc tcctggagaa cattactttg
      481 caactgaggc ggctaatggc gaactgggtt tttacattgt atctgaaggg gacaagtctc
      541 cctggagagt tcatgtcaga agaccctgtt tctggtacta tcagtcattc ccgcagcttg
      601 tgaaagggtc tctgattgca gattcaatcg ctacaatgag ttctttaaat gtgatcgcag
      661 gggagttgga ctgttaatat ggcttatcaa ttttctcagg aatcggaagg caagtttcaa
      721 aaactgattc ctatgtttcc ggacaaacgt tctttgattc ttccggctct ctatcttctt
      781 cagaaagatc agggatttgt ggatacagtc gggatgcagt acattgcaga cagaattggt
      841 gctccaatag tactttctca cgtatatgga gttgctacat tctacacttt atacaataag
      901 aagcctgtag gaaagtatca tatacaggtc tgtgcaaata tttcctgctg cgtgatgggt
      961 tccgacagaa ttacgaacac agtctgcaaa aagcttggaa tcaaaaaagg agaaaccact
     1021 ccggataaaa agtttacagt ggatgaagtt cagtgccttg gtgcatgcgg gtacggtccg
     1081 atggctcaga tcaatgacca gtactatgag tttttggatg agaaaaaaat tacagaaatc
     1141 ttggattcac tggagtaaag aatggctgaa ctgaaaatcc tcacaaaaca cagaggagaa
     1201 aaggatactc atacaatttc gcattaccag tctgtaggcg ggtatactgc agtgaaaaaa
     1261 gcactttcca tgcagggaga cgatattgtt gcaacagtga agaattccgg tctcagagga
     1321 agaggcggtg caggttttcc tacagggatg aaatggtctt ttattcctaa gaatatcaac
     1381 aagccgaaat accttctttg caacggagat gaaggcgagc ctggaacttt caaagacagg
     1441 gttctccttg aggagttccc gcacgcaatg atagaaggta tgatcattgc agcgaaggcg
     1501 attgattctc atcagggata tatctatatt cgcggggaat accacaaaag catcagccga
     1561 gtggagcagg caattaatga agcgtatgca gcggggcttc tcggaaaaaa tatacagggt
     1621 tcaggatttg attttgaact ttgtgtatac cagggtgccg gtgcctatat ctgcggagaa
     1681 gagactgctc tgattaattc actggaaggc agaagaggtc atccaagact gaagcctcca
     1741 tttccggcag tatccggact ttacggatgc cctacagttg taaacaatgt ggaaaccttc
     1801 agtgcagttc ctcatatcat caacaacggt gcagaatggt atgccaaaat aggaactcct
     1861 aagtccactg ggactagact tttcagcgtg agcggtcctg taaaaaaacc aggagtttat
     1921 gagatcccgc tgggaacacc tctgatgaca ctgatcaacg atctttgcgg aggaatggca
     1981 gatggaaaga aactgagagg aatcattcct ggaggttctt ctgttcctat tctcactgcg
     2041 gaagagtgca agactgcaaa tatggacttt gaatcaatgg cagaacataa aacgatgctc
     2101 ggttcgggtg cagtcattgt tctggcagaa gatactgata tcgtagaaac tacattccgt
     2161 cttgcagagt tttatgcaca tgagtcctgc ggtcagtgta ctccctgccg tgaaggaact
     2221 cattgggtag cagatcttct gcataagatc agggatgggc atggaaccaa aaaagacttg
     2281 gaactgattc tgtctttgac agtaaatatg gaaggcggga caaccatctg tccattggca
     2341 gatgcctgtg taggagctgt tcgacctacc atccagaaat accgtccaga gtttgaagca
     2401 aggctcaagg gctaaaaaag cggactttta ggagcgttgc aagctcccga atactccggc
     2461 aggaaataaa tctgggtctg aagcagtaat attcagatcc ggcaggataa aaaagacgga
     2521 cttttaggag cgttgcaagt tcctagataa tccggtagga taaaaaaagc ggggtcttag
     2581 gggttgcaaa cccctaaacc tatccggtag gatcattatg gattggaatt tagttttagt
     2641 ttgggctctg aaaagcggga ttctgttttt tatcattatc accgcatgtg catattacac
     2701 acttgctgag agaagggttt gcggctggat acaggacaga ccagggccga atcgtgctgg
     2761 cccttttggg ctttttcagc ctcttgcgga cggtgtgaag tttctgacga aggaagaggt
     2821 ttttcctaca aatgtgaaca aagggctcta tctgattgca cctgctattt ctgtttcctg
     2881 tgcgattatg gcctggtcga tcattccatt cggaggaaca atccctcttc ccgcagacct
     2941 tgctaaggca gtaggatttg acaagttgga tcttcaggtg gcaaacccgg acaccggaat
     3001 tctatttctt ctggccattt cctctctggc cgtttacggg atcattctgg caggctggag
     3061 cagcaacaat aagtattcat tgatgggagg aatcagatcc actgctcaga tgatcagcta
     3121 tgaacttcct ctcgggcttg cactgacttc catcatactt ctttccggtt cattgaggct
     3181 gacggatatc aatgatgcgc agaaagggat gtggtttatt ttcactcttc ccggttttat
     3241 cgcattcatc attttttccg tggcgatgtt cgcagagaca aacagactcc cgtttgacct
     3301 tgcagaggct gaatcagagc tggttgtagg atttcatact gaatacggtg cattcaaatt
     3361 tgcattattc tttattgctg aatacatgaa tatgctgacg atgagctgcg tagtgacttt
     3421 gctctttttc ggaggataca atcttcctct cggaattctg aaaggatccc agtttgaagc
     3481 acttgcaggc cttggatttt ttgcagtaaa agtgattttc tttgcattct gttttatctg
     3541 ggtcagatgg acactgcctc gtttccgtta tgatcagctg atgaatatcg gctggaaaaa
     3601 gcttatccct tgggcattgg ccaatattgt tctggcagga acctatgtgg tttactgggg
     3661 caaatactgg aagggaatat tcaactgata tgatattaga tttaaaccat cctcaatctt
     3721 tattctttct gatttttgcg ggaattacag taggttctgc acttgcggta gttctgcata
     3781 aaaatgcagt agtttcagcc gcatctcttg tactttcctt ttttgcactg gcggggattt
     3841 atgctgtgat gaatgcagtg ttcattgcca ctatgcaggt gctcgtgtat gcgggtgcca
     3901 tcatggtgct tgtgattttt gtaattatgc ttctttcact gcgcgaagag actggaaagg
     3961 aaattttcag aaaacccatc aaacagactt tcatttttct gcttgtggtt gtattcggag
     4021 cccttctgat cactgcctcg aattacgctc ctgatgtagg cagaaattct gcagacagag
     4081 tgaaggctgt accctactca tatacactta agcaggatgc aaaggctcct gttcagtcaa
     4141 aaggaaacac tgcaacagtc ggagcatcca catttatcga ctatcttctt ccgtttgaac
     4201 ttgtatccat tctgcttctg gcagctgtag tcggtgctgt cctgcttgca aaaagagaaa
     4261 aaattaaaaa tcaggggggc gcactttgaa ccatcttatt tccggaattc cactggggta
     4321 tttcttaagt cttgccggaa ttctgttttc catcggagtg tttggagtcc ttttcagaag
     4381 aaatgcagtc gtaattttta tgtctgtaga actgatgctc aattctgtca atttggtatt
     4441 catttcattt tccaaagcaa tgtctcaggt ttcaggtgag gtcattgtat tctttgtaat
     4501 ggcaattgca gctgcagaag ctgcgatagg tcttgcaatc gtaatagcca tccacagaca
     4561 taaaaagaca gtttatgtgg atgaaatcaa tcttttgaag tggtgaaaat atgctggatc
     4621 tttttccttt agtagttctt cttcctctcg cgggtttttt aatcaacggg ttttttcata
     4681 ggaaaattcc gcatactgca gcggcagtca tagggatcag tgcggtagca gtccctttct
     4741 tcattacgct cggtgcactg tttgaatttc agcccttggt gagaaaaaca cctcatctct
     4801 tttccattct ttcctggatt catgcaggaa agtttgctgc agactttgcc taccaggtgg
     4861 atcagctttc attgtatatg acactgatta tcactggaat cggatctctg attcatctgt
     4921 acagtgtggg ttatatgcac ggagaatccg ggttcaacag atttttcgtt tatctgaatc
     4981 tgttcatttt ctccatgctg aatcttgttc tgggcgacaa ccttgttctg atgtttctcg
     5041 gatgggaagg agtaggagtc tgttcttatc ttctgatcgg atttgatttt cataaatctt
     5101 ccgcagcaaa tgcagggatg aaagcgttta tcgtaaacag gatcggtgac gtaggttttg
     5161 ccgtagggat cttccttacg tactggtatc tgggaagcgt caagtatgtg gatattatga
     5221 ctgcacttcc gacagcgctt ccattcaagg aaatcatcaa ctgggtggca ttggcattct
     5281 ttatcggggc gattggaaaa tcggcgcaga tcccacttta tgtctggctt cccgatgcga
     5341 tggccggtcc tacccctgtg tctgcactga tccatgctgc aacgatggtg acagcgggcg
     5401 tgtttatgat cgcaagactg aatcctatct tttatgcggc agaacacgca agcacatata
     5461 ttgcatttac aggagcagtg actgctctgt ttgctgcgac aatcggtctt ttccagacgg
     5521 atatcaaaaa agttctcgca tattccactg tatcccaatt gggatatatg ttccttgcga
     5581 tgggggtcgg tgcgtacacc gcaggaatgt tccatctgat gactcatgca ttcttcaagg
     5641 cactgatgtt cctcggttcc ggttctgtga tccatgcaat gcatcacgag caggatatga
     5701 gaaatatggg taatctaaag aatcata
//
LOCUS       DAOQGJ010000023         5644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_7211_length_5644_cov_3.732152, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000023 DAOQGJ010000000
VERSION     DAOQGJ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7211_length_5644_cov_3.732152"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK683_00705"
     CDS             complement(<1..221)
                     /locus_tag="PK683_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH06991.1"
                     /translation="MEETATENQLLTFYCGAEVFAIDILKVKEIKEYTELTSIPLMPK
                     AVQGVINLRGQVLPIIDLNIRFGRPAAKIT"
     gene            complement(224..3250)
                     /locus_tag="PK683_00710"
     CDS             complement(224..3250)
                     /locus_tag="PK683_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH06992.1"
                     /translation="MIKLNKIKSLILLTGLLSAFVFALPGGVSLLLISSSSSRMESAL
                     QKEISEISSMRDILDLYAVTVVDTVHKLRAKNVSWAESRSAVKKARKEISKKTEETLQ
                     KFQGSDAVKIKDAVKDADQKLDILEEILLKEDSAVLEDFGKNILYPAIDPVTAAFSSA
                     INSKIENAKSTVSTFAELSRNYIYIVFSILAFGILAVFLFVKYISGLILNPLHLVKAK
                     IRSLSLGNFSNETVQYDADNEIGDLVKEFNGLIQKFQTILKDTFFAKAGLDSASSNIM
                     MADNDLKIIYMNSSVRKMFTEAENEIREQIRGFSASRLMGSSIDSYHRDPEVQRRILK
                     DLKGSHRSRIQIGKKTFDLLANPVFSETGERLGSAVEWTDVTKMLELESERERSFAEN
                     FRIRTALNSVSTNIMVADNDCNVIYMNNSIKKMFSEAETDIREQVRFFDSSRIMGNSI
                     DPYHADPAHQRRILKNLNGTHRSRIKIGKRTFDLSANPIITDSGEKLGSVVEWADVTE
                     LLKQETEKMRTAAENMRIRTALDNVSTNIMIADNDQNIIYVNKAVLRMFTAALSDIRK
                     QFPAFDPSSLLGMSIDRFHKNPHHQRGILGSITGEYKSSIEIGGRAFNLVANPVLDNS
                     GNRLGSVVEWTDYQAEIIVQKEIDGIINKAIAGEFDSRIVQEGKTGFFKNIAEKINTL
                     LDISSAGISDVAAVLNAVSTGDLTKRVDKDYKGIFGELKEYTNRTSSKILEIITEVKS
                     NADAVILAADQVSLTADNLSQSASAQAASVEETSASLEEMNANIGQNAENARQTNIIA
                     GRTSSDATEGGKSVSETVKAMRQIAQKIGIVEDIAYQTNLLALNAAIEAARAGEHGKG
                     FAVVASEVRKLAERSQVAANEIGELASVSVSIAENAGRLISEIVPAINKTADLVQEIT
                     ASSSEQASAVEQISKAVSQLDGVTQQNASSSEELASTSQQMALKAENLSKLINFFKLS
                     ENENREHTGRPSAEAPQSSSVKLMTTVPTSENYERY"
     gene            complement(3265..5502)
                     /locus_tag="PK683_00715"
     CDS             complement(3265..5502)
                     /locus_tag="PK683_00715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNH06993.1"
                     /translation="MDIDLTEVREIFFSEAADLLGKMENALLEIQSGSDTVSQEKINE
                     LFRSVHTIKGSAGIFEFKYIVEFTHIVENVLDRLRSLQIPMTPELISVLIKCRDHLSD
                     LVELEVQNRQPDSEMHEVQNSLMTRLSEFSDKKLEIKTSDSAVLLNQKEISVPSETVS
                     ENRETAGSEFSQKDGLWHISLRYEKDTFRNGLDPASVFRYLKKNGEIVNLYTITAAIP
                     EISSFDPSECHFGFELLYRTDRDMEFIQDAFEFVQYDCSIKIFPPDRNPEDFTAYVLS
                     RKGKESVLRLVRIFLLMGILDRRQALLLKDYRKKERRLSREKKETSVASEDHLKESSS
                     SANEETKKNPPARENRIVRIDSAKLDQLVDFVGEIVIAGASLSQMALQKKDSQLSEAV
                     SNLNRLVSEIRDVSLNLRMVQIGDTLSKYNRVVRTLSEELGKEIQLFISGGETELDKA
                     VVEKINDPLMHIIRNAIDHGIETAEERKALGKPVKGRLSLHAYHETGSIVIEVSDDGK
                     GLDREKILKKAAEKGLISAEKSMSDEEIFRLIFQPGFSTAEKITNISGRGVGMDVVSR
                     NIEALRGSVTVFSEFGKGTMLQIRLPLTLAIIDGFLFRVGDANFVIPLDNIVECIEFS
                     KKGEDSDGKNFINLRGEILPYLILADVLGIPESRTSRKNVLVLQYAGKKAGVVVDTLH
                     GELQTVIKPLGKIFQNLKGISGSTILGTGEVALLLDIGAFFKRVAEIENRHWKKKTEI
                     TAGLN"
BASE COUNT         1318 a         1301 c         1142 g         1883 t
ORIGIN      
        1 gtaatttttg ccgcaggtct gccgaagcgg atattgagat caattatcgg aagaacctgg
       61 cctctgagat tgattacacc ttggacagct ttcggcatca atggaatcga agtgagttct
      121 gtgtattcct tgatctcctt gactttcaga atatcgatgg caaacacttc agctccgcag
      181 taaaaggtca ggagctgatt ttcggtggca gtctcttcca tttctaatac ctctcgtagt
      241 tttctgaagt gggtaccgtt gtcatcagtt ttacactgct gctctgagga gcttcggcgg
      301 aaggtcttcc cgtatgctct ctgttctcat tctcactgag cttgaaaaaa ttgatgagtt
      361 tactgaggtt ctctgccttg agagccatct gctgagatgt cgatgcaagt tcttctgaag
      421 aggaagcatt ctgctgagtc actccgtcca gctgagaaac agccttgctg atctgttcca
      481 ctgcagatgc ctgttcactg ctagatgcag tgatttcctg aacaagatct gcagttttat
      541 tgatcgctgg gacgatttca ctgatcagtc ttcctgcatt ttccgcaata cttacactga
      601 ctgaggcaag ttccccaatt tcatttgcag ccacttgact tctctccgca agttttctga
      661 cctcgctggc aactacggcg aatcctttgc cgtgttcgcc tgctcttgcc gcttcaatgg
      721 ctgcattcag tgcaagaaga tttgtctggt aagcgatgtc ttccacaatt cctattttct
      781 gtgcaatctg gcgcattgcc ttgactgtct cactgactga ctttcctccc tctgtagcat
      841 cactggaagt acggccggca atgatgtttg tctgtcttgc attttctgca ttctgcccga
      901 tattcgcgtt catttcttcc agagaggcac tggtttcttc cacgctcgct gcctgtgcac
      961 ttgcactctg actcagattg tccgcagtga gactgacctg gtcggctgcc agaatgactg
     1021 catctgcgtt tgattttact tctgtgatga tttccaggat tttggaagat gttctgtttg
     1081 tgtattcctt cagctcaccg aaaatacctt tgtaatcctt gtctactctt ttggtaagat
     1141 ctccggttga aacagcattc agaacagctg caacatcact gattcctgca gaactgatgt
     1201 ccagaagtgt gttgattttt tctgctatgt ttttaaagaa tccggttttt ccttcctgca
     1261 caattctcga atcgaattca cctgcgattg ccttattgat gattccgtca atctctttct
     1321 gtacaattat ttccgcctga tagtcggtcc attccacaac gctgcccagt ctgtttcctg
     1381 agttgtccag tactggattt gctacaaggt tgaatgctct tcctcctatc tctatggagg
     1441 atttatattc acctgtgatg gatccgagta ttcctctctg atgatgcgga tttttgtgga
     1501 acctgtcaat ggacattccc agcagcgaag aaggatcgaa tgcgggaaac tgctttctga
     1561 tatccgaaag ggccgctgtg aacattctca gcactgcctt gttcacatag atgatattct
     1621 gatcgttgtc tgcaatcatt atatttgtgg agacattgtc cagagccgtt ctgattctca
     1681 tattttctgc ggcagtcctc attttttctg tttcctgctt cagcagttca gtcacatctg
     1741 cccattccac cacgctgccc agtttttctc ctgaatcggt gatgatcgga tttgctgaga
     1801 gatcaaatgt tctttttccg atttttattc tggatcgatg tgttccgttg aggtttttca
     1861 gaattcttct ctgatgcgca ggatctgcat gataagggtc gatggaattt cccataattc
     1921 tggaagaatc aaaaaatctg acctgctctc tgatgtctgt ttctgcctca gaaaacattt
     1981 tttttatgga attattcatg taaattacat tacagtcatt gtctgccacc ataatgtttg
     2041 tcgagactga attgagtgcg gttctgatcc tgaaattttc tgcaaatgat ctttctctct
     2101 ctgattcaag ttcaagcatt tttgtcacgt cggtccattc gactgcactg cccagtcttt
     2161 cgcctgtttc tgaaaataca ggatttgcaa gaaggtcgaa tgtctttttc ccgatctgga
     2221 ttctggatct gtgggaacct ttcaggtctt tcagaattct tctttgaact tcaggatctc
     2281 tgtgatagga atcaatgctg cttcccataa gtctggatgc actgaatcct ctgatctgct
     2341 ctctgatttc attttctgcc tcagtgaaca ttttccttac tgaactgttc atataaatga
     2401 ttttaagatc attgtccgcc atcataatat ttgaggatgc gctgtcgagt cctgccttgg
     2461 cgaagaaagt gtccttgagt attgtctgga atttctgaat gagtccgttg aattccttta
     2521 caagatcccc gatttcattg tccgcatcgt actgaactgt ctcatttgaa aaatttccga
     2581 gagaaagact tctgatcttt gcttttacaa gatgcagagg gttcagtatc aggcctgata
     2641 tatattttac aaaaaggaag actgcaagta tgccgaatgc caggattgaa aatacaatat
     2701 aaatgtaatt tctggaaagt tccgcaaatg tgctcactgt gcttttggca ttttctattt
     2761 tggagtttat tgcggaagaa aacgctgctg taaccggatc aattgcggga tacagtatat
     2821 ttttcccaaa atcttcaaga actgcactgt cttccttgag cagtatttcc tcaagaatat
     2881 cgagtttctg atcggcatcc ttgactgcat ccttgatttt aactgcatca gatccctgga
     2941 atttctgcag agtctcttct gtttttttcg aaatttcctt tctggctttt ttgactgcag
     3001 atctggattc agcccagctg acattctttg ctctgagctt gtgcacggta tccacgactg
     3061 tcactgcata cagatcgagg atgtccctca tggaactgat ttctgatatt tccttctgca
     3121 gtgcagactc catcctcgaa gaactggagc tgataagcag aaggctgact ccgcccggca
     3181 gggcaaatac aaaggcagac agtaatcctg tcaataatat caggctcttt attttattta
     3241 attttatcat ttatttttct ccattcagtt caatcctgca gtgatttctg tctttttttt
     3301 ccaatgcctg ttttcaatct cggcaactct tttaaaaaag gctccgatgt ccagaagaag
     3361 tgccacttcc cctgtgccta aaatagtgct tccgctgatt cctttcagat tctggaaaat
     3421 ttttcctaac ggtttgatga cagtctgaag ttcaccgtgc agagtatcca caacaacgcc
     3481 tgcctttttt cctgcatact gaagaaccag tacattcttt ctggaagttc tgctttccgg
     3541 aatgcccagc acatccgcca ggatcagata gggaagaatt tcgcctcgca gattgatgaa
     3601 atttttcccg tcggaatcct ctcctttttt tgagaactca atgcattcca ctatattgtc
     3661 gagagggatt acaaaatttg catctcccac tctgaaaaga aatccgtcaa tgattgccag
     3721 tgtaagagga agtctgatct gcagcattgt tccttttccg aattctgaaa ataccgtgac
     3781 acttcctctc agagcctcaa tgtttctgct gactacatcc attccgaccc ctcttcctga
     3841 aatgttcgta atcttttctg cagtggaaaa acccggctga aaaatcagtc tgaatatctc
     3901 ttcgtcggac attgacttct ctgcagagat aagtcctttt tctgccgctt tcttcagtat
     3961 tttttcgcgg tcgagtccct ttccgtcatc actgacttca atgacgatgc ttcccgtttc
     4021 gtggtatgca tgaagtgaga gtctgccttt gacaggcttg ccgagcgctt ttctttcttc
     4081 agccgtctcg attccatggt caattgcatt tcgtatgata tgcataagtg gatcatttat
     4141 cttctctacg actgctttgt ccagttcagt ttcaccgcct gaaataaaga gctggatttc
     4201 ctttcccagt tcttctgaaa gagttcttac cactctgttg tattttgaaa gagtatctcc
     4261 tatctgaacc attctgagat tgagggatac gtctctgatt tcactgacca gtctgttcag
     4321 attggaaacc gcctcagaca gctgagagtc ctttttctgg agtgccatct ggctcagact
     4381 tgcacctgca atgacaatct ctcccacaaa gtctacgagc tgatcgagtt tggcagagtc
     4441 aatccgtaca attctgttct ctcttgcagg agggtttttc tttgtttctt cattggcgga
     4501 tgaactgctt tccttgagat gatcttcact ggcgaccgaa gtctcttttt tttctctgct
     4561 gagtctcctt tctttttttc tgtaatcttt cagtaaaagt gcctgccttc tgtccagaat
     4621 tcccataagt aaaaatattc tgacaagacg gagaacagat tcttttcctt ttcggctgag
     4681 aacataggca gtaaaatctt ccggatttct gtcgggagga aatatcttta tgctgcagtc
     4741 atactgaacg aattcaaatg catcctgaat gaattccata tcacggtctg ttctgtaaag
     4801 aagctcgaat ccaaaatggc attctgaagg atcaaatgag cttatttccg gaattgcagc
     4861 agtgattgta taaagattta caatttcccc gttttttttc agatatctga aaacggaagc
     4921 cgggtcgaga ccattgcgga atgtatcctt ttcatatctg agagagatat gccataaccc
     4981 gtctttctgt gaaaactcag atccggcagt ttctctgttt tccgaaacag tttctgaagg
     5041 aactgaaatt tctttctgat tcagaagaac tgcagaatcg gaagttttta tttccagttt
     5101 tttgtctgaa aattcggaaa gtctggtcat cagactgttc tgaacttcat gcatttcact
     5161 gtccggctgt ctgttctgca cttccagttc caccagatca gagagatgat ccctgcattt
     5221 aataagcaca gatatgagtt caggggtcat gggaatctga agcgaacgga gccggtcaag
     5281 tacgttctcc acgatatgag tgaattctac tatgtacttg aattcgaaaa tacctgcact
     5341 gcctttgatc gtatgaacag accggaacag ttcattgatt ttttcctgac tgactgtatc
     5401 ggatcctgac tgaatctcca gaagtgcgtt ttccattttt cccagaaggt ccgccgcttc
     5461 ggaaaagaaa atctctctga cttccgtgag atctatatcc atttcagagg attcctttag
     5521 aaggtctctt catcccgtac cgaaaagaat acttttctct gtctgactga ctgattctga
     5581 ttctgtcctc aaagaagccg acaaggccgt atatatccag aagatccaga agtgccgggg
     5641 aatg
//
LOCUS       DAOQGJ010000024         5604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_7295_length_5604_cov_3.289962, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000024 DAOQGJ010000000
VERSION     DAOQGJ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7295_length_5604_cov_3.289962"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            495..695
                     /locus_tag="PK683_00720"
     CDS             495..695
                     /locus_tag="PK683_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06994.1"
                     /translation="MKLLDEQLDTANREKYKKTNQTDAKKIHQCRGCGAQVEKLYQQN
                     LCRQCLSAAFKRLIKVIDSVRK"
     gene            710..3301
                     /gene="leuS"
                     /locus_tag="PK683_00725"
     CDS             710..3301
                     /gene="leuS"
                     /locus_tag="PK683_00725"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNH06995.1"
                     /translation="MSYPFREIEEKWQDIWEKKNTFKTDIGSKKPKYYCLDMFPYPSG
                     AGLHVGHPEGYTATDIISRFKRMKGFEVLHPMGWDAFGLPAERYAMTHGIHPRITTEQ
                     NVNNFRRQIKSLGLSYDWSREISTTDPSYYRHTQWIFLKIYNSYFDHREKKAKPISGL
                     IQEFEQSGSSLFFTEQSGTDAHKFTAEEWKAKSPVDKEKILSGQRLVYSAEIPVNWCE
                     ELGTVLANEEVEEWTAKGYSVVRKPMRQYMMRITAYAERLLEDLSLVSWPQSTLEMQK
                     NWIGRSEGCEIEFRTESNDPIPVFTTRPDTIFGVTYLVLAPEHPLVKKITSQGQSAEV
                     ESYAKAASLKSDLDRTELNKEKTGVFTGSYALHPFTGEKIPVWISDYVLYHYGTGAIM
                     AVPAHDERDFSFAKIMKLPIRHVIAGADSEEVCFDSKESKCINSKSDLLDINGLSYKE
                     AFDKTASVLEKKGIGRKRLQYKLRDWLFARQRYWGEPIPLVHSSEGLTFDIPESELPL
                     TLPELEDYKPDKSGESALAKATSWLKYESSGKTGRRETNTMPQWAGSCWYYLRFIDPV
                     SRSIFCKSELEQKWMPVDLYVGGAEHAVLHLLYSRFWHKILFDLGYVSTPEPFKKLVH
                     QGLILGEDKRKMSKSLGNVVNPDDVIKEFGADSFRLFEMFMGPLEMSKPWNTRGVEGV
                     FRFLNRTWRLFHKEDGTFFTDDTEPDKEELKILNRTIQKVEVDIENLSFNTAISQMMI
                     FVNEMTPKPNRPWKILRPFVLLLAPFAPHLAEEIWSKFGYKNSLAHERFPYVDTAYLE
                     DDTMVIVVQVNGKLRDQIEVSKTMEQSEIISLAKKAEKIPQYIEGKKMIKEIYVKEKL
                     VNFVVGN"
     gene            3328..4002
                     /locus_tag="PK683_00730"
     CDS             3328..4002
                     /locus_tag="PK683_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH06996.1"
                     /translation="MVKKKYLLEMDRGNYALALTILDSEIQNADPPDPADLYNFAVCC
                     VRTGNYKKAIQVIEDLITHYLKFIEMDNAYRLMIYSYIQIGEYQTALRITDERLKVNV
                     GDLRLLSFRAHILEKLGRMTDSIQIHRNILKIRPDYINSLNSLAFLLCAQEKVSQSDL
                     DEATDCIRKALKQNPDNAAYLDTFGVLLKTKGNKDQAIRAFNKALAIAPQAGAEILDH
                     LKELLD"
     gene            complement(4004..4675)
                     /locus_tag="PK683_00735"
     CDS             complement(4004..4675)
                     /locus_tag="PK683_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06997.1"
                     /translation="MNRIRLLYLILPVILGCSSTPSASSSSNPPSKTSQPTVTTAPKA
                     SDSPAKKATKTFQPAGTKERILDQAGEEIEITDLDPEQFWAPSKDPNEHFRVVLSSDS
                     YQLRQIRGSHAMRKKPDPGGDQLLMEEVAKYDQADFKDDGIIIVNLNSKTGKIEKVNF
                     HTRLPRIHDLAKIMQNDLTRWNLEHKKEDAPEITRFTVSYYIILRSKASRTDVKEMLK
                     KEVRR"
     gene            complement(4802..4939)
                     /locus_tag="PK683_00740"
     CDS             complement(4802..4939)
                     /locus_tag="PK683_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06998.1"
                     /translation="MITDWLFQGDHTIKGIAVIALIISILVFAYAVEKIAEGGRRGKK
                     Q"
     gene            complement(4936..5235)
                     /locus_tag="PK683_00745"
     CDS             complement(4936..5235)
                     /locus_tag="PK683_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH06999.1"
                     /translation="MKSALKMELTVANSVLQKTLHILDAAGMNYTVFKDIAGKGEKGS
                     KSAENVLTVMTCTYIVSVSSPEKIEAFLESATELLETFGGTYFLSEVTGWDRIAL"
     gene            complement(5394..>5604)
                     /locus_tag="PK683_00750"
     CDS             complement(5394..>5604)
                     /locus_tag="PK683_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07000.1"
                     /translation="GNWLNTVKSILEDRLSAKKKRTDMTEAASEAMVVFVGASMLYRM
                     TGQMEFLNAMGSQFEKIADKVKSLR"
BASE COUNT         1635 a         1169 c         1205 g         1595 t
ORIGIN      
        1 actgtcaaga taattgcaca gaattcagtc atttagattc agttctctga tgaactgtct
       61 tcagtgaatg ccctcagttc ggcatatacg gaagcagttt ctgaaaaaag tccctttttc
      121 ataactgaac tgtctttcaa tcatatcagc ataattctaa tttaggatat tcgaattttc
      181 ttatatgatt tctgtccttt aaaatcagca tatttaagaa gaaactattg tgctgaaact
      241 gcaaaaaata aaaagtaaca ttgctaattc agttttctct tatatgcttt ttaagaatcg
      301 ttccatatgc tctttatttt ttgaatgagt ttataaaaaa atttcgtaaa aaaatgaaga
      361 gagggcagct atattctctt tgacactgca ggctcctgca aatatatcgt ttccattccg
      421 ctaaaaaaag ttttcggaaa cggaaactta atgcacagag ttatttacat actgtaaaaa
      481 acaggatata aagaatgaaa ttattggatg agcagttgga cacggcaaac agagaaaaat
      541 acaagaaaac aaatcagaca gacgcaaaaa aaattcatca gtgcagaggc tgcggcgcac
      601 aggtggaaaa actttatcag cagaacctgt gcagacagtg tctctcagca gcattcaaaa
      661 gactcataaa agtcattgac tctgtaagaa agtaattcac agatgttcta tgagttaccc
      721 ctttcgcgag atagaagaaa aatggcagga catttgggag aaaaaaaata ccttcaagac
      781 tgacataggc tcaaaaaagc cgaagtatta ctgtctggat atgttccctt atccgagcgg
      841 tgcaggtctg catgtaggac atccggaagg atatacagcg acagacatta tttcccgatt
      901 caaaaggatg aaaggattcg aagtccttca tcctatgggc tgggatgcat tcggtctccc
      961 ggcggaaaga tatgcgatga ctcacgggat tcatccccgg atcactacag aacagaacgt
     1021 aaacaatttc agaagacaga tcaaaagtct gggactttct tacgactggt ccagagagat
     1081 ttctactaca gatccttcct attacaggca tactcagtgg atttttctga agatttataa
     1141 ttcttatttt gatcatagag aaaagaaggc aaagcctata tccggactga ttcaggagtt
     1201 tgaacagtct ggatcttcac tgttttttac agaacagagc ggaacagatg ctcataaatt
     1261 cactgcagaa gaatggaagg caaaatctcc ggtagacaaa gagaagattc tttccggtca
     1321 gcgtcttgta tattctgcag aaattccagt caactggtgc gaagagctcg gaacagttct
     1381 ggcaaacgaa gaagtggagg agtggactgc gaagggatac tctgtagtgc gcaaacctat
     1441 gcgtcaatat atgatgcgga ttactgcata tgcggaaaga cttctggaag atctttctct
     1501 tgtaagctgg ccccagtcta cgcttgagat gcagaagaac tggatcggca gaagcgaagg
     1561 gtgcgaaatt gaattcagaa cagaatcgaa tgatccgatc cctgtcttta ccacaagacc
     1621 ggatacaatt ttcggagtca cctatctggt tctcgcccct gagcaccctc tggtcaaaaa
     1681 gattacatcc cagggacagt cggcagaggt ggaatcctat gcaaaggctg catctctcaa
     1741 gtctgatctg gacagaaccg agctgaacaa ggaaaagact ggtgtattta ccggcagtta
     1801 tgcgctccat cctttcactg gagaaaaaat ccctgtctgg atttccgatt atgtacttta
     1861 tcattatgga acaggtgcga ttatggccgt tcctgcacat gatgaaaggg atttttcttt
     1921 tgcaaaaata atgaaacttc ctatacgcca tgtgatcgca ggagctgatt cagaggaggt
     1981 atgctttgat tcaaaggaat ccaaatgcat caattccaag tctgatttac ttgatatcaa
     2041 cggtctttct tataaggaag cctttgataa gactgcgtcc gttctggaaa aaaaagggat
     2101 cggaagaaaa agacttcagt ataaactcag agactggctt tttgcaagac agagatactg
     2161 gggagaacct attcctctgg tacattcctc tgaaggactg acattcgaca ttcctgaatc
     2221 ggagcttcct ctcactcttc ctgaactgga agattacaag ccggacaaga gcggtgaatc
     2281 ggcgttggcc aaggcgacat cctggctgaa gtatgaatct tccggaaaaa caggtcgcag
     2341 ggaaacaaat acgatgcctc aatgggcggg atcctgctgg tactatctcc ggttcatcga
     2401 tcctgtctcc agaagcatat tctgcaagtc tgaactggaa cagaaatgga tgcctgtgga
     2461 tctctatgtg ggcggtgcag aacatgcagt tcttcatctt ctctattccc ggttctggca
     2521 caaaattctg tttgacctgg gttatgtttc cactcctgag cctttcaaaa agcttgtgca
     2581 tcagggactt attcttggag aggacaagag gaaaatgtcg aagtctctcg ggaatgtcgt
     2641 caatcctgat gatgtcatta aagagttcgg tgctgacagt ttccgtcttt tcgaaatgtt
     2701 tatgggcccg ctggaaatgt ccaaaccttg gaataccaga ggagtggagg gagtattccg
     2761 ttttctgaac agaacctgga gactttttca caaggaagac ggcacttttt ttacagacga
     2821 tactgagccg gacaaggaag aattgaagat tctgaacaga acaatacaga aggtggaagt
     2881 ggacattgaa aatctttcat tcaatactgc aatttcacag atgatgatat tcgtgaatga
     2941 gatgactcct aaacccaaca gaccctggaa gattctccgc ccgtttgttc tccttctggc
     3001 tcctttcgct cctcatcttg cagaggaaat ctggagcaag ttcggttata agaattctct
     3061 tgctcatgaa agatttcctt atgtagatac tgcctatctg gaagatgata caatggtgat
     3121 cgtcgtgcag gtgaacggaa aactcaggga tcagattgag gtttcaaaaa caatggagca
     3181 gtccgagatc atctctcttg ccaaaaaggc agaaaaaatt cctcagtaca tcgaaggaaa
     3241 aaaaatgatc aaagagattt acgtgaagga aaagcttgtc aactttgtag tgggaaatta
     3301 atctgcacag ggctggtgca ataatttatg gtgaagaaaa aataccttct tgagatggac
     3361 aggggcaatt atgctctggc attgaccatt cttgattcag aaattcagaa cgctgatcct
     3421 cctgatcccg cagacctgta caattttgca gtctgctgtg tacggacagg aaattataaa
     3481 aaagcgattc aggtaattga agatctgatc actcattatc tcaaattcat agagatggac
     3541 aatgcctaca ggctgatgat ctattcttat attcagatcg gagaatatca gactgctctc
     3601 agaatcactg atgaaagact taaagtgaat gtgggtgatc tgcgccttct gtctttcaga
     3661 gcccatattc tggaaaaact gggaagaatg accgattcca ttcagattca caggaacata
     3721 ctcaagatca gaccggacta tatcaacagt ctgaactcac ttgccttcct tctgtgcgct
     3781 caggaaaaag tatctcagtc ggatctggat gaggccactg actgcatcag aaaggcactg
     3841 aaacagaatc cggacaatgc tgcttatctg gatacattcg gggtgcttct taaaacgaag
     3901 ggaaacaagg atcaggcaat ccgtgctttc aacaaagcat tggcaatagc tccgcaggct
     3961 ggtgcggaga tactggacca cctgaaagaa cttctggact agattatctt ctgacttcct
     4021 ttttcagcat ttctttcaca tcagttctgc tggccttgct tctgagtata atataataac
     4081 ttacagtaaa tcttgtgatt tcaggagcat cttctttttt atgttccaga ttccatctag
     4141 tcagatcgtt ctgcataatt tttgccagat catgaatccg aggaagtctt gtatggaaat
     4201 ttactttttc gatttttcca gtttttgaat tcagattgac aatgatgatc ccgtcatcct
     4261 tgaaatccgc ctgatcgtat ttggcgactt cttccatcag aagctgatct ccgcccggat
     4321 ccggtttctt tctcatagcg tgagaacctc tgatctgtct cagctgatac gaatcactgc
     4381 tcagaactac tctgaaatgt tcgttcggat cctttgacgg tgcccagaac tgctcagggt
     4441 cgagatcagt gatctcaatt tcttctccgg cctgatcgag tatcctttcc ttcgtaccgg
     4501 caggctgaaa ggtctttgta gccttttttg caggactgtc ggatgctttc ggtgcagtgg
     4561 tgactgtcgg ctgtgaggtt ttggaaggcg gattggaaga agaggatgcg gacggagtgg
     4621 aagagcatcc caaaataaca ggaagaatta agtacaagag tctgattcta ttcatacatt
     4681 caacaaatct gcatgcttga cagagtcaat cattttagat ttgcgaatta tcgtattgta
     4741 cagtttcctt gtctctgaat ttgactgcag agaccggagc gcatctgctc cgtccttaat
     4801 cttactgctt cttgcctcgt ctgcctccct cagcaatctt ctctacagca taggcaaaca
     4861 ccagtatgga tataatcaga gcaatgaccg caatcccttt tattgtatga tctccctgaa
     4921 acagccagtc ggttatcata acgctatcct gtcccagccg gtgacttctg aaagaaagta
     4981 agttcctccg aatgtttcca gcagctctgt tgccgattcc agaaaagctt caattttttc
     5041 cggagaggat actgatacaa tgtatgtgca tgtcattact gtcagaacat tttctgcact
     5101 cttgcttcct ttctctccct tgcctgcaat atccttgaat actgtataat tcattcctgc
     5161 cgcatcgagg atatgaagag tcttctgaag aacagaattt gcaacggtca attccatttt
     5221 taatgcactt ttcatgaaag ttctcctgaa aaattgaaaa agcatttttg attcagaaag
     5281 tttccacgtt ttaattttag gtccatattt tcctccggaa cttaggagaa gtatggctca
     5341 tttatggagc aaaatcgtcc tgacttacaa actgaaacgt ttttttgacg attttaacgg
     5401 agagatttta ccttgtctgc gattttttca aactgggaac ccattgcatt cagaaattcc
     5461 atctgtccag tcatcctgta cagcatggag gctccaacga aaacaaccat tgcctcggat
     5521 gcagcttccg tcatatctgt acgctttttc tttgcagaca gtctgtcttc cagaatggat
     5581 ttcactgtat tgagccagtt ccct
//
LOCUS       DAOQGJ010000025         5463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_7579_length_5463_cov_3.214497, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000025 DAOQGJ010000000
VERSION     DAOQGJ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7579_length_5463_cov_3.214497"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1578)
                     /locus_tag="PK683_00755"
     CDS             complement(142..1578)
                     /locus_tag="PK683_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07001.1"
                     /translation="MSDFSNDSDSSKTEMRFLFFILILFSTALNAKNPKTMHGVFPAQ
                     DYRQFWFLYESEKRPGQNQWFFRPFYSHYKEEQSAHQFNTSLYPLYYREKTNHWSKWT
                     FMFLFGQDTTVHPDTGEDDDLALSPLFQWGRGETDRDRYFAVFPFFGNINSKLGWQKI
                     HYAMFPIYTSWEHKDFKAHSFLWPLTMYGTSDVRQEYRLFPFFTKKTHIGKFEHYNVF
                     WPFVSWGRDSLDKREPTSYIFLWLLYAHKNSYYGNMKSFGIMPVLGSMALFSWGHDRR
                     SSETNVNLLFFLIQYGSSEDKDYRKHIFFPFYGYSRFASKEFRFITPLFIKLNTDSYL
                     VESESWYFLPVFHYNREYFRKEERTDTYYKVWPFWKWHKDTEGNLSTNLLSLFPFRSE
                     SMERLWEPVWSVFEYKSQINGEKRVSLLMRLYSQRWSEEEFHFYVPLVMDYSNTKDSF
                     SMKFLYGLLGYEKKDSSSTYQFLWFLKI"
     gene            1730..2215
                     /locus_tag="PK683_00760"
                     /pseudo
     CDS             1730..2215
                     /locus_tag="PK683_00760"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
     gene            complement(2223..3032)
                     /locus_tag="PK683_00765"
     CDS             complement(2223..3032)
                     /locus_tag="PK683_00765"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNH07002.1"
                     /translation="MIKLLFSIFLSVSVSAQSYMLGEKLEYKIIWGFMDVGTSVMSVL
                     PEPVEYNGVMCHVFKSMAKSHGFMDSMYHVDDKIVSYFDPVTGRTMSAAKRIHEGKFH
                     REYHADFDHKLHAVSWWSKHFKGKQPDGENQRFRSGLKKEIPSFILDALSAIYYTRNH
                     SEEPVPGKEFTLPVYDDLEVTTLQITVLREEEIELTVNGVQKKYQAFVAKPFIPTSGI
                     FKSEGEILIWISKDKRRIPLKIQSKVNIGNVTVELDKTENLIEEEPLETGK"
     gene            3291..3638
                     /locus_tag="PK683_00770"
     CDS             3291..3638
                     /locus_tag="PK683_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH07003.1"
                     /translation="MPEFSFKGKTYINPVEFALDMIGGKWKMPILWRLREKTLRYSEL
                     KNDLPNITHKMLTSQLRELEENGFVDRKVYPVVPPKTEYCLTARGKKCLPVIEALRKY
                     GNELKKELKAESV"
     gene            3686..4684
                     /locus_tag="PK683_00775"
     CDS             3686..4684
                     /locus_tag="PK683_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNH07004.1"
                     /translation="MQEILRLQPIYKEKIWGGRKLESFFQRNIPGGKIGESWEVSDYG
                     KDLSVIQNGILKGKTLREAYRNDPEGILGKSKIPFREFPLLVKIIDAQDRLSVQVHPD
                     DRYAEEKDPESSGKKEAWYILQSDPKSEIVCGFSESLNRDSYRLMIERNEAEKPLQTF
                     NAEKGNAFMITPGTIHAIGKGNLILEVQQSSDSTYRVYDYGRIGDDGKPRPLHIQKSL
                     DVLNFQKSDLSEFLKGHKADWKFGERKILNRNDKFFFEILKSNDNVFLPGVSDRPLFR
                     IITVISGRVNVSEETFLAGDSFILTAEGTEKNLSFQFSKNTEIALMGPGSDWCEYE"
     gene            4816..>5463
                     /locus_tag="PK683_00780"
     CDS             4816..>5463
                     /locus_tag="PK683_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH07005.1"
                     /translation="MKKTISLICLVFLSNCLKSKKSAFDISAGGFSFFAFYGFLLSEA
                     FQIKTVTVPGTSSKASLIPDITILFSAEPDSSSVTESTVKVIDKDGKQISADLGISDK
                     TLTVSVSSILSVSSDYQLKISSQVKDKSGRNLGSDYSTSFTTDSGTDHFLKDSSLNND
                     LISPLGYTVSTRLFSFSDRLYIAWTEKADANIGNNALVYVKSIDKNKSVSSVGTNL"
BASE COUNT         1757 a         1096 c         1076 g         1534 t
ORIGIN      
        1 tgattccggc aatatacatt tggtgtataa tttcattacg cttgaaaaaa atggcgcgaa
       61 gctgaagaaa cgtctggaat agaaggatca gcgtatatcc cgcaccgtat actgtttcag
      121 agaatatttt tatcaggaat ttcatatttt taaaaaccag aggaactgat atgtggatga
      181 ggagtctttt ttttcataac ccaataagcc atataaaaac ttcatggaaa aactgtcctt
      241 tgtattgctg taatccataa ccagaggaac ataaaaatga aactcttctt cggaccatcg
      301 ctgagaatac agtctcatca gaagagaaac ccttttttct ccgttgatct gacttttgta
      361 ttcaaataca gaccagacag gctcccaaag tctctccata ctttccgaac gaaacgggaa
      421 aagagaaagc agatttgtgc tgagatttcc ttccgtatcc ttatgccatt tccagaaagg
      481 ccagaccttg taataagtgt ctgttctttc ctcttttcga aagtattctc tgttgtaatg
      541 aaaaacaggc aggaaatacc aggactctga ttccaccaga taagaatcgg tattgagctt
      601 aatgaaaagg ggagtgatga accgaaattc cttggaagca aatctggaat aaccgtaaaa
      661 gggaaaaaat atatgcttcc tatagtcttt gtcttcggaa gaaccatact ggatcaggaa
      721 aaagagcaga ttgacgttgg tctcggagct gcgtcggtca tgtccccagc tgaaaagagc
      781 cattgacccg agtacaggca taatcccgaa acttttcata tttccataat aggaattttt
      841 atgagcatag agaagccaga ggaaaatata ggaagtaggt tctcttttgt ccagactgtc
      901 ccttccccag gaaacgaaag gccagaatac attataatgc tcaaactttc caatgtgtgt
      961 ttttttggta aaaaaaggaa acagtctgta ttcctgccgt acatcggaag ttccgtacat
     1021 cgtcagcggc cagaggaagg aatgtgcctt gaaatccttg tgctcccaac tagtataaat
     1081 cggaaacatt gcataatgta tcttctgcca gccgagtttg gaattgatat ttccgaagaa
     1141 cggaaacact gcaaagtatc tgtctcgatc cgtttcccct cttccccact gaaacaaagg
     1201 agaaagagcc agatcatcat cctcgccagt atccggatga acagtcgtat cctgtccgaa
     1261 aagaaacata aacgtccact tgctccagtg atttgtcttt tctctgtagt acaaaggata
     1321 gagagaagtg ttaaactgat gagcactctg ctcttccttg taatggctgt agaacggacg
     1381 gaaaaaccat tgattctgcc ccggcctttt ctctgattca tacagaaacc agaactgtct
     1441 gtaatcctga gcaggaaaaa ctccgtgcat cgttttagga ttctttgcat tcagtgcagt
     1501 agaaaaaagt atcagtatga aaaacaggaa gcgcatttcg gtcttggaag aatctgaatc
     1561 attgctgaaa tctgacaaga aataaaactg aaatttgaaa gttctttgca gacaaaagaa
     1621 ttggaattga ctttgcatct gagtttgtca gtatttgcct atgacggcag attccttaca
     1681 attcagagca gaaagaattt tcaggtatga tcttcgtaca taccatctga tgggagaact
     1741 tggcctgatt cccagaaata cagagctgat ttacggagta gttgttcata aaatgacgat
     1801 atcaccgatc cattccagaa cagtaaccaa gctggccaat aacctgtcta gaaatcttcc
     1861 tgacagtttt atcgtcagac aggaaaaacc aatctcgata aatgactcag aaccggaacc
     1921 ggatatttca gtcatcaaag gaacatacga cgacttcgga gaaagacatc cggaaactgc
     1981 tgatctggtg attgaagctg cttaaagctc gatggaagat gatctggata aggcttctat
     2041 ttatgccggt ccagatattc ctctatactg gattattgac atcaaaaaca aaaagcttca
     2101 tatctttgaa aagctggata agggaaaata cagcatacat acaatattag aatccaatat
     2161 ctgtgtaaaa attccatata cctcaaaaga aatctccctg gatgagattt tgtaactgaa
     2221 ttttatttcc cggtttccag aggttcttcc tctattagat tttctgtttt atccagctcc
     2281 acagtcacat ttcctatatt gaccttggac tgaattttca aaggaattct tctcttgtct
     2341 tttgagatcc aaatcagaat ctctccttca gatttaaaaa tacctgaggt gggaataaag
     2401 ggcttcgcca caaatgcctg gtattttttc tgtacaccgt ttactgtaag ctcaatttct
     2461 tcttctctca aaacagtaat ctgaagagtg gtcacttcca gatcatcgta caccggcagt
     2521 gtaaattcct ttcctggaac cggttcctct gaatgatttc tggtataata aattgctgag
     2581 agcgcatcca gaataaaact gggaatttct tttttcagtc cggatctgaa tctctgattt
     2641 tccccgtccg gctgcttgcc cttgaaatgc tttgaccacc agctcacagc atgaagctta
     2701 tgatcaaaat ccgcgtggta ctctctgtga aatttgcctt cgtgaattct ctttgcagca
     2761 ctcattgttc tgccggtgac aggatcaaaa taactcacga ttttatcatc cacatggtac
     2821 atggaatcca taaatccgtg agactttgcc atacttttaa atacatggca catcacgccg
     2881 ttgtactcta caggttccgg gagcacactc atcacagaag tgcccacatc cataaaaccc
     2941 cagataattt tatattccag tttttcgcca agcatatagg actgagcgga aacggaaaca
     3001 gataggaaaa tactgaaaag cagcttgatc atcaatgatt agacataaat ttttgaagaa
     3061 gcagagatct ttttttgaat ctgaaaatcc cctgcttcag aatgctctgc tttcagacat
     3121 aaatcggcac agctttttac tgaactgtcc agaaagtttc agcaatttaa accggttttg
     3181 gcgggacaga ttgtttatgt cattttaatc tgatgcacag agagtgataa ataatacttg
     3241 ttaatttata taatgcaaaa aggattatac tatcatagag gaaagcattt atgccggaat
     3301 tttcattcaa gggtaagaca tacataaacc cggtggagtt tgcactggat atgatcggcg
     3361 gtaaatggaa aatgccgatt ctatggagac tgagagaaaa aactcttagg tacagcgaac
     3421 tgaaaaatga tcttccaaac atcactcata aaatgctgac cagtcagctt cgtgaactgg
     3481 aagaaaacgg gtttgtagac aggaaagtgt atccggtcgt tcctcccaaa actgaatact
     3541 gcctgactgc cagaggaaaa aaatgccttc cggtcataga agcgcttcga aaatatggga
     3601 atgaactgaa aaaagaactg aaggcagaat cagtttgatt taaaattcac ttgatctgaa
     3661 taaaacccca ttttccccta gacttatgca ggaaatactc agacttcagc cgatttacaa
     3721 agaaaaaatc tggggtggaa gaaaattgga atcttttttc caaagaaata ttcccggagg
     3781 aaaaatagga gaatcctggg aagtttccga ttatggaaag gatctgtcag tcattcaaaa
     3841 cggcatactg aaaggaaaaa ctcttcgtga agcataccgg aatgatccgg aaggaatatt
     3901 aggaaaatct aaaattccct tcagagaatt cccgctgctc gtaaaaatta ttgatgcaca
     3961 ggacagactc tcagttcagg ttcatcctga cgacagatat gcagaagaaa aagatccgga
     4021 aagttcagga aaaaaagaag catggtatat ccttcagtct gatccaaaat cagaaatagt
     4081 ctgcggattc tccgagtctc tgaacagaga ttcatatcgg ctgatgattg aaaggaatga
     4141 agcagaaaag cctcttcaga cattcaatgc agagaaaggg aatgcattta tgattactcc
     4201 cggcacaatc catgcgattg gaaaaggaaa tcttatcctt gaagtgcagc agtcttctga
     4261 ttccacgtac cgcgtttatg actacggccg tattggggac gacggaaaac caagacctct
     4321 gcatattcaa aaatcactcg atgttctgaa ttttcaaaaa tcggatcttt ctgaattttt
     4381 aaaaggccat aaagcggact ggaaattcgg agaaaggaaa atactcaacc gaaacgacaa
     4441 gtttttcttt gagatattaa aatctaatga taatgtattt ctgcctggag tttcagacag
     4501 acctctgttc aggattatca ctgtcatcag cggcagggtc aatgtatccg aagaaacatt
     4561 tctggctgga gacagcttca ttctgactgc agaagggaca gaaaaaaatc tctcttttca
     4621 gttcagcaaa aatacagaaa tcgcactgat gggacctggt tctgactggt gcgagtatga
     4681 atagaaacaa attattgtct gattgaaatt gctgaagcaa aaccttggat ataagttttt
     4741 aagaaaacag taaaaattta ttaacccaag accgcagtct tggaagctgc ctataaagat
     4801 ccgtctaata tgaatatgaa aaaaacaatt tcactgattt gtcttgtgtt tctttcaaat
     4861 tgtctgaaat cgaagaaatc tgcatttgat atctctgccg gtggtttcag tttttttgca
     4921 ttctatggat ttcttctgtc tgaagctttc cagataaaga cagttacagt gcctggtaca
     4981 tcatcaaagg caagccttat ccctgatata accattctgt tttctgctga accggattca
     5041 tcgagcgtca ctgaaagcac ggtaaaagtc atagacaagg atggaaaaca gatttctgca
     5101 gatctgggca tctctgataa gacactcact gtttcagtgt cctccattct ttccgtaagt
     5161 tcagactacc agctgaaaat atcttcacag gtaaaggaca aatccggaag aaatcttgga
     5221 tcagattaca gcacttcatt cactacggac agcggcacag atcattttct caaagactcc
     5281 agtctgaata atgacttaat atctccttta ggatatactg tatccactcg actgttttct
     5341 ttctctgaca gactttatat agcctggaca gaaaaagcag atgcaaacat tggaaacaat
     5401 gcacttgttt atgttaagtc cattgataaa aacaaatcag tctccagtgt tggaacaaat
     5461 ctg
//
LOCUS       DAOQGJ010000026         5453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_7600_length_5453_cov_3.578733, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000026 DAOQGJ010000000
VERSION     DAOQGJ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7600_length_5453_cov_3.578733"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PK683_00785"
     CDS             complement(<1..980)
                     /locus_tag="PK683_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNH07006.1"
                     /translation="MDRLSAKNGHFTDSRGNIILLRGVNLSGSSKVPFFPDGNTAYDQ
                     GASFSSPESVSFVGRPFPIEEAEEHLERLKRWGFNFIRFIITWEAVEHGGPGIYDEDY
                     IEYIGQIVSIAEKKGIYLFIDPHQDVWSRFTGGDGAPAWTLETVGIDVRKLPSTSTVI
                     LHHVRKENYRRMSWPLNYQKYPAASMFTVFFGGNFFAQGLKVDSMPIQDWLQERYISA
                     CLRIAVRIAKNKNVVGFDSLNEPSPGWIGRKSLSSFEGIGAGVAETPSPFQEMCLSEG
                     VPISVFNSFLFGTVRVPFGIKKLGEKNISIWKKDSHCIWRKHGVWDYDPHG"
     gene            1077..2576
                     /locus_tag="PK683_00790"
     CDS             1077..2576
                     /locus_tag="PK683_00790"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07007.1"
                     /translation="MKRLPIGIQTFSEIITENYLYVDKTDRILELIKNGKYYFVSRPR
                     RFGKSLLISTLDSIFNGRKELFQGLYIYEKLEWKKYPVIRLDLSLVSGKDPNQLETSL
                     SFRLSQIAEQNGIVLHSEYCTDRFQELILGLSKNGKAAVLIDEYDKPIIDHLGNPAVA
                     EQNREILRNFFGVIKGCDEYLRFVLLTGVSKFSQVSIFSGLNNIRDITLSKESSSLFG
                     YTEEELKMYFRDRIAELAASFGVSNDEFMKKIQKWYNGYSWDGIIKVYNPFSIMMFFN
                     EGAFRNYWFESGTPSFLVSYIKDHHMEIPKIEKTEITYETFSSFALDNIQFSSLLFQT
                     GYLTVVKSASFEYQQYFVLDYPNEEVRNSFFTHITASFLKDRTPAEVDPVILEMRRYL
                     QKEDLKLFFEKLQSLFASVPYTLHLPYEAYYHSLFYMVLRLLGFRTETEVLTDKGRID
                     GIMELNDKIYIIEIKLDRPEKAIEQIRGRKYAEKFIGSEKKSSCSESDI"
     gene            complement(2735..3370)
                     /locus_tag="PK683_00795"
     CDS             complement(2735..3370)
                     /locus_tag="PK683_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002739740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNH07008.1"
                     /translation="MSGELYEKYRKKFSVKYLFPIMTGLSLFVHVGIYGAYYIYQNWK
                     PDEVDSSTITQEVDMLEDIPPELLGGDKSPAPVEKKDWVEGTKKNGDAPDDSDIKDAL
                     SGNGTDKDGFMFAVHGDRVPTPIMEFDLKNYFPQAAKDANITDKQVILLVQIDESGHL
                     VSAKIVSGRAGYGFDDAAMQVIKLVRWVPGYVKGRPVKMTHRVPINFSLNE"
     gene            complement(3373..3777)
                     /locus_tag="PK683_00800"
     CDS             complement(3373..3777)
                     /locus_tag="PK683_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH07009.1"
                     /translation="MAMSSGGNEDEIIDSINITPMVDVILVLLVIFMVTANFLKKESI
                     NINLPKVEAADPNVTKSTQVALGRDGKIYLEGTVVNEEKLSSVLTRDAKLKPNMRLTL
                     SADEKIPYGTIMKLMGVIRKTGVTRVALSVKR"
     gene            complement(3789..4412)
                     /locus_tag="PK683_00805"
     CDS             complement(3789..4412)
                     /locus_tag="PK683_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNH07010.1"
                     /translation="MGQLELVTFGEYAVFVLMGIASVIAVAVGLERAVVFNKSSAKSA
                     GIIKSIVERLRKKEYGALAEVETSSPKNVYARFASFGSEHKDSGAESLSELLAAKAIE
                     ERIYLERRLPILSTLGNNAPFLGLLGTVLGVIKAFYGMGALGASGAEVVMKSISTALF
                     ATAAGLFVAIPVVMVNNFFSKKLKVISQNLDIISKEFLASLGSGQNK"
     gene            complement(4422..4904)
                     /locus_tag="PK683_00810"
     CDS             complement(4422..4904)
                     /locus_tag="PK683_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07011.1"
                     /translation="MKHILYVIIFITSLTCSGKNHSDYQDSYNSRIVLMSLIAAKPNP
                     QGACETASKSAGSCIFKAKDRSALADIYGEEIYASSILSSNRQQSYTDYCKNQLSSST
                     YEKMSDAYRECSFNCMKQSWDAAVNAKSCEKFNTADLITTAVNGMKSCLIKCAKPTNN
                     "
     gene            complement(4921..>5453)
                     /locus_tag="PK683_00815"
     CDS             complement(4921..>5453)
                     /locus_tag="PK683_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07012.1"
                     /translation="SNADPSAGRILTSDELRLRSMWNNSRETLGDYDVTHLLSVVFGW
                     RINADYQVGGRWFYRTAFPVTPVIGDDGGQFRNPANGQTFWNPQYSNNPYSAEYVNSR
                     RLPDYHRLDFRFDKFMNYEWGFINLYFEVINVYMRQNTVGEILTRPDHSQKPTLLLSM
                     TFSFSGTTDGFLLSST"
BASE COUNT         1475 a         1226 c         1123 g         1629 t
ORIGIN      
        1 ccgtgaggat cgtaatccca tacaccgtgt ttcctccaga tgcagtggct gtcttttttc
       61 cagatggaaa tatttttttc ccccagtttt ttaatgccga agggaactct gactgttccg
      121 aacagaaatg aattgaatac gctgatcggc actccttcgg aaagacacat ttcctgaaaa
      181 ggggagggtg tctccgccac tcctgcaccg attccttcaa aactcgaaag actctttctt
      241 ccaatccatc ccggtgaagg ttcattcagc gaatcaaagc ccactacatt tttatttttt
      301 gcaatccgta cagcaattct gaggcaggca gaaatgtatc tttcctgaag ccagtcctgg
      361 atcggcatac tgtccacctt cagaccctga gcaaaaaaat tccctccgaa aaatacagta
      421 aacatggatg cagcaggata cttctggtaa ttgagcggcc agctcattct tcggtagttt
      481 tcctttctga catgatggag aatcactgtg gatgtggacg gaagttttct cacatctatc
      541 ccgaccgttt ccagtgtcca ggccggagca ccgtctccac cggtaaaccg cgaccataca
      601 tcctgatgcg gatcaataaa aagataaatt cctttttttt cagcaatact tactatctga
      661 cctatatatt ctatatagtc ctcatcatat attcccggac ctccgtgctc tactgcttcc
      721 caggtgatga tgaatcggat aaaattgaag ccccagcgtt tcagtctttc cagatgttct
      781 tctgcttctt caattggaaa cggtcttcct acaaaggaaa cagattcagg actgctgaag
      841 gatgctccct gatcgtaggc tgtgtttccg tcaggaaaaa aaggaacctt ggaggaaccc
      901 gaaagattca ctcctctgag aagaataata ttgcctctgc tgtccgtgaa atgaccgttt
      961 tttgcgctga gtctgtccat cactggacag ttttttgcat atgcagatga aaatcaactt
     1021 caaacctgca ctgaaatact tcttgattct catatgagca gtgagagttt ggaagggtga
     1081 aaagacttcc gatagggata cagacgttct cagaaattat tacagaaaat tatctgtatg
     1141 tggacaaaac ggacaggata ctggaactga taaagaacgg taaatactat tttgtatcga
     1201 gaccgagaag attcggcaag tcacttttga tcagcacgct ggattcaatt tttaatggaa
     1261 gaaaagaact ttttcaggga ctttatatat atgaaaaact ggaatggaaa aaataccctg
     1321 taatccgtct ggatctttca cttgtttcgg gcaaggatcc gaatcagctg gaaacttctc
     1381 tttccttccg cctttcgcag attgcggagc agaacgggat tgtgctccat tccgaatact
     1441 gtacagaccg ttttcaggag cttatactgg gtctctcaaa aaacggaaaa gctgccgtgc
     1501 tgattgacga gtatgataag ccgattatag atcatctggg aaatccggca gtcgcggaac
     1561 agaaccggga aattcttcga aattttttcg gcgtcatcaa gggctgtgac gaatatctga
     1621 gatttgttct cttgacaggg gtttccaaat tttcccaagt ctccatcttt tccggcctga
     1681 acaatatcag ggatatcaca ctctcaaaag aatcctcctc tcttttcgga tatacggaag
     1741 aggagcttaa aatgtatttt cgggacagga ttgcagaact tgcagcttct ttcggagtaa
     1801 gtaatgatga attcatgaaa aaaattcaaa aatggtataa tggttacagt tgggatggca
     1861 taatcaaggt atataacccc ttctccatta tgatgttttt taatgaaggg gctttcagga
     1921 attactggtt tgagtcaggc actccttctt ttcttgtcag ttacataaag gatcatcata
     1981 tggagattcc aaaaatagaa aagacagaaa ttacatatga gacattttcc tcctttgcac
     2041 tggataatat tcagttcagt tcactcctct ttcagaccgg gtatctgact gtcgtcaaat
     2101 ctgcctcttt cgaatatcag cagtactttg ttctggacta tcctaacgaa gaggtcagga
     2161 attccttttt tacgcatatc acagcttcat ttctaaagga cagaaccccc gcagaggttg
     2221 accctgttat tctggaaatg agaagatatc ttcagaaaga agatctgaaa cttttttttg
     2281 aaaaacttca gtctcttttt gcgtctgttc cttatactct tcatctccct tacgaagcat
     2341 attatcattc tcttttctat atggtgctcc ggcttctcgg attcagaaca gaaaccgagg
     2401 tgcttaccga caaaggccgt atagacggaa tcatggaact gaatgataaa atatatatca
     2461 ttgagatcaa actcgacaga cccgaaaagg caattgagca gattcgcgga aggaaatatg
     2521 cagaaaagtt cattggttct gaaaaaaaat catcctgctc ggaatcggat atatagacag
     2581 agatatcagt ttcatcaggg aagactttta ctgataaaac cggtcagtta atttcaaccg
     2641 ccctgagaca ggaaatctga acagtttctg aggagatcaa gtttcccggc aggttccctc
     2701 tttgcttccg caaaaaaaca aagcctgccg aaaactactc attcagtgaa aagttgatcg
     2761 ggactcggtg agtcatcttt accggtctgc cttttacata gccgggcacc catctcacaa
     2821 gttttatcac ctgcattgca gcatcatcaa atccgtatcc tgcacggcct gatactatct
     2881 ttgcactgac tagatgacct gactcatcta tctgcacaag aagaatgacc tgcttgtcag
     2941 taatgttggc atccttggct gcctgaggaa aataattctt cagatcaaat tccattatgg
     3001 gagtagggac tctgtcacca tgaactgcaa acataaaccc gtccttgtct gttccgttcc
     3061 cagagagagc atccttgata tctgaatcgt ccggagcatc tccgtttttc tttgtacctt
     3121 ccacccagtc ctttttttcc accggagcag ggcttttatc gcctcccaga agttccggag
     3181 ggatgtcttc aagcatatcc acttcctggg tgattgtgct ggaatcgact tcatcaggct
     3241 tccagttctg gtatatataa tatgcaccgt atatcccgac atgcacgaaa agggagagac
     3301 ctgtcataat tggaaacaga tattttacag aaaatttctt tctgtatttt tcatatagtt
     3361 cgccggacat atctacctct tcactgaaag agctactctt gtgactcctg tctttcggat
     3421 gactcccatc agcttcataa ttgttccgta cggaattttt tcatctgcag aaagggtcag
     3481 cctcatattc ggcttgagtt ttgcatctct tgtcagaaca gaagaaagtt tttcctcatt
     3541 gactactgtt ccttccaggt agatttttcc gtctcttccc agtgcaacct gggtggattt
     3601 tgtaacattc ggatcagcag cttccacttt gggaagattg atgttgatag actctttctt
     3661 cagaaagttg gcagtgacca tgaatattac aaggagaacc agaatcacat ccaccattgg
     3721 agtaatgttg atactgtcta tgatttcgtc ttcatttccg ccggatgaca ttgccataat
     3781 aagcctcctt atttattctg gccggaaccg agactggcca ggaactcttt ggatataata
     3841 tccagatttt gggatatgac tttcagtttt ttggaaaaga agttgttcac catcacgaca
     3901 ggaatggcaa caaaaagacc tgcagcagtt gcgaaaaggg cagtagagat acttttcatt
     3961 acaacctctg ctccggatgc accgagtgct cccattccgt aaaatgcctt gattacccca
     4021 agcacagttc ctagaagtcc aagaaaggga gcattattcc caagagtcga gagaataggg
     4081 agtcttcttt ccagatagat cctttcttca atagccttgg ctgccagaag ttcagaaaga
     4141 ctttctgctc ctgaatcctt gtgttcagaa ccgaaagatg cgaatcttgc atagacattc
     4201 tttggactgg atgtttccac ttctgcgaga gctccgtatt ccttttttct gagtctttcc
     4261 actattgact tgatgatccc tgcagatttt gcagaacttt tattgaatac gactgctctt
     4321 tccagtccca ctgccaccgc gattacgctt gcaattccca ttaagacgaa gactgcatat
     4381 tctccgaatg ttacaagttc cagctgtccc atattttctc ctcagttgtt tgtaggttta
     4441 gcacatttaa ttaaacagga tttcattcca ttgacggcgg ttgtgatcag atccgcagtg
     4501 ttgaattttt cacaggattt tgcatttaca gcggcatccc aggactgctt catacagttg
     4561 aatgagcatt ccctgtaggc atcgctcatt ttttcataag tcgatgaact cagctgattt
     4621 ttgcagtagt ctgtgtaact ctgctgtctg tttgagctca gtattgaaga ggcataaatc
     4681 tcttcaccgt aaatgtcggc aagggccgat ctgtcctttg ccttgaatat gcagcttcct
     4741 gccgatttag acgcggtttc gcaggctccc tgcggattcg gctttgcggc gatcagagac
     4801 atcagtacga tcctggaatt gtagctgtcc tgatagtcag agtgattttt tccggaacag
     4861 gttaatgatg taatgaatat aattacatat aatatatgct tcaaaatctg acctccattc
     4921 ctacgttgaa gaaaggagaa atccgtccgt tgttcctgag aaggaaaaag tcatactgag
     4981 gagaagggtt ggtttttgag aatggtctgg tcgtgtcaaa atttcaccga ctgtgttctg
     5041 tctcatataa acgtttatga cctcgaaata cagattaatg aaaccccatt cataattcat
     5101 gaacttgtca aatcggaaat ccagtctgtg atagtcagga agtcttctgg aatttacata
     5161 ttctgcagag taggggttgt ttgaatactg cggattccag aaagtctgtc cgtttgccgg
     5221 attcctgaac tgtccgccgt cgtcacctat tacaggcgtc acagggaatg cagttctgta
     5281 gaaccatctt cctccgacct gatagtctgc atttattctc catccgaata ccacactcag
     5341 caggtgggtg acatcatagt cgcccagcgt ctctctggaa ttgttccaca ttgaccggag
     5401 acgaagttca tcgcttgtga gaattctccc agcactcgga tctgcattcg aat
//
LOCUS       DAOQGJ010000027         5435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_7648_length_5435_cov_3.559294, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000027 DAOQGJ010000000
VERSION     DAOQGJ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7648_length_5435_cov_3.559294"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..2075)
                     /locus_tag="PK683_00820"
     CDS             complement(84..2075)
                     /locus_tag="PK683_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07013.1"
                     /translation="MSNPKKIYLGKQKLKSIPEEIFEKTYITELNLNCNEISVIPESI
                     GKLKNLKKLYLMGNPVTALPESMQELKKLEVLDLSGTQLKILPLFLFSMLHLKKISLW
                     GTSLEYIPEEIGNLKGLTSLSVWTDRLPDSIGSLTDLVKLNLTGKNLNSLPSQIGNLI
                     SLESLFINHTNIENLPDSFGNLQNLKTADLSANSRLRDLPASLGKLKRLEKLNLLFCD
                     LKFVPEEIGYLNSLKELQIQTASLPTSIGKLNSLEILVLSSLKSSELPKEIEYLIHLK
                     TLKILDTQAEFIELDFGKFRNLNDLQISRNPNLKGISGSIGKSSLEQIYFNDNPMWKH
                     LPEELGQCQNLEDIEMIYSHFEKLPASLSQLKSLKKLRFAWNENLKDLPFSEEQVMSL
                     EYLDLTENPNLLVPSYFQKIPSRILSSSSKNFIMKLYPSIYSSKQEIADKMISELNDG
                     IEHFEFPYWDDYSTYHSAAGKMRVSAFHSEEGMALIFESIEYNSGTGEIWDCAKGFAT
                     FSISRRMPNIENSIRAAMDGFKLERDMIYNLELNDEVFPISLETEFIEFCESRSGSSG
                     DKPPDEAILLFKIAETVPVEKLFPDIREIRKAFEIPETMEHMYSVTDWLHPTADDIFG
                     ENKKMEEFESIVELINAAAEHRRPVLKEEPNTDWKQMFC"
     gene            2361..2672
                     /locus_tag="PK683_00825"
     CDS             2361..2672
                     /locus_tag="PK683_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HNH07014.1"
                     /translation="MPKKTSRAVETLSSKQEWTFLSNHAHVLICLYRDENVRLRDLAF
                     NVGITERAVIRIIEDLEAANVLVRFKEGRRNKYKIFDNIPLRHKLESHKTIGDLLKLL
                     K"
     gene            3102..3782
                     /locus_tag="PK683_00830"
     CDS             3102..3782
                     /locus_tag="PK683_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNH07015.1"
                     /translation="MKIKHISAVLAFCLCVPVSVQAEISPDEALNRLTEGNRRFMQGK
                     SIHPNQTPEKIKELSSGQRPFAVIVGCSDSRVPNEIIFDQGLGDLFILRTAGQVSTYA
                     SWGSIEFATAVLGAKLIVVLGHTKCGAVAAACSVPDVPGHIVTLINAIKPGVEIAREM
                     KGDLVDNAVKINVAREVEQLKNLEPVLSKQFKKGEIRIVGAVYHLETGKVEILPDNFL
                     KNFGEDKK"
     gene            3782..4759
                     /locus_tag="PK683_00835"
     CDS             3782..4759
                     /locus_tag="PK683_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNH07016.1"
                     /translation="MEMIDSLMNGLMTPMVLAFALGIFATLIKSDLKIPEGLYIGLTI
                     YLLLAIGIKGGVKLSSTPMADFLKPAMIAVLMCSLIPVWSFFILNRMGKFSAADSAAV
                     AAHYGSVSVVTFSAAQTFLEQKGISFEGFMPTMLAIMEIPGILVAIFLARIHIKNDKS
                     SYKKLFHELFTGKGTVLLIGGLIIGMLSGKKGFEQVSPLFDTPFKGVLTLFLLEVGMV
                     TGRKLEDLKKAGVFLFCFAVLMPLLHSSIGISLGKLIGLSMGGSVIFGVLCASASYIA
                     APAAVRIAIPEANTSYYLTASLAITFPFNIVLGFPIYTNIAKMIYGVNT"
     gene            4756..5058
                     /locus_tag="PK683_00840"
     CDS             4756..5058
                     /locus_tag="PK683_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07017.1"
                     /translation="MNLSIVQLVTIIALDSLEDTLIQDLKALGMKGYTVSEAHGEGLS
                     TRRDDNWEGRNIRIEILARQELAESILEFLQKEYFPKYKMIAFLQEVKVLRKEKFH"
BASE COUNT         1655 a         1051 c         1101 g         1628 t
ORIGIN      
        1 ggaatctttg taggaaatga atctacaaca aatctgtttt cattcaaaaa aatgtaactg
       61 atcatcggaa aacagggagc tgttcagcag aacatctgtt tccagtctgt gttcggctcc
      121 tctttcagaa ccggtctgcg gtgttcagcg gctgcattta taagttcgac tatggattca
      181 aattcttcca tctttttatt ttctccaaaa atatcatccg ctgtcggatg aagccaatca
      241 gtgacagagt acatatgttc cattgtttcc ggaatttcaa atgcttttcg gatttccctt
      301 atgtctggaa acagtttttc aacaggcaca gtctctgcaa ttttgaaaag aagaatagct
      361 tcatcagggg gtttatcccc ggaactgccg gaacgagatt cacaaaactc aataaattca
      421 gtttccagag agatcggaaa cacttcatca ttcagttcta aattatatat catatctctt
      481 tctaacttga atccatccat cgcagcccgt atcgaatttt ctatattcgg cattcttctt
      541 gaaatactga atgttgcaaa accttttgcg cagtcccaaa tttcacctgt cccggaatta
      601 tattcaatag attcaaatat aagtgccata ccttcttcag aatgaaaagc tgaaactctc
      661 atcttgcctg cagcactgtg atatgtggaa tagtcatccc agtagggaaa ttcaaaatgt
      721 tcaattccgt cattcagttc tgagatcatt ttatctgcaa tttcctgttt tgaagaatat
      781 atactaggat ataatttcat tataaagttc ttagacgagg aggaaagaat tctggaagga
      841 attttctgaa aatatgatgg aaccaggaga ttcggatttt cagtcaaatc gagatattca
      901 agactcatta cctgttcttc tgaaaatggc agatccttca gattttcatt ccacgcaaaa
      961 cgcagttttt tcagtgattt cagctgagac agagaagcag ggagtttttc aaaatgagaa
     1021 tatatcattt caatatcttc cagattttgg cactgcccta actcttcagg cagatgcttc
     1081 cacataggat tatcattaaa atatatttgc tctaaagaag attttcctat agaaccggaa
     1141 atacctttta aattgggatt tctcgatatc tgaagatcat tcaggttccg gaattttccg
     1201 aaatccagtt caatgaactc agcctgagta tccagaattt tcaaagtctt taaatgaatc
     1261 agatactcaa tttctttagg cagctccgag gatttcaaag aggaaagaac aagtatttcc
     1321 agggagttga gttttccaat cgacgtgggc agtgaagcag tctggatctg aagctctttc
     1381 agcgaattca gataaccgat ttcttccgga acaaatttca gatcacagaa aagaagattg
     1441 agtttttcca gtcttttgag ttttccgaga gaggcgggaa gatctctcag cctgctgttt
     1501 gcagaaagat cagccgtttt gagattctga agatttccaa atgagtccgg taaattttct
     1561 atattcgtat gattaatgaa taatgactcc agtgaaatca gatttccgat ctgagaaggc
     1621 agtgaattca gattttttcc tgtcaaattc agtttcacca aatctgtcag gcttccgata
     1681 gagtcaggga gacggtcagt ccatacagaa agagaagtca gtcccttcaa atttccgatt
     1741 tcttccggga tatattctaa tgatgttccc cagagactga ttttcttcaa atgcagcatc
     1801 gaaaataaaa aaagagggag aattttcagt tgagttccgg aaagatcaag cacctccagt
     1861 ttcttcagtt cctgcatact ttcaggaaga gcggtaacag gatttcccat aagataaagt
     1921 ttcttaagat ttttcaattt tccgatagat tccggaatga cagaaatttc attacagttc
     1981 agattcagtt ccgtaatata agttttttca aatatttcct caggaatgga tttcagcttc
     2041 tgttttccta aataaatttt ctttggatta ctcattttgt tttgaaagaa ttctcagtgc
     2101 agagattcca tcctccagtc ttattttcgg caggaaaagt tttttaccgt atttcattct
     2161 tgactcctgc tcagcaaacg agccttggca gtaccatata gaggcacagt aaaaccgaaa
     2221 gagacagtcg gaacttagaa agcagttatc gcatcatctt ttttcatatt gttctgcact
     2281 gctccaaatt tatcaggaat agaaaaattt ccaattctat tgacaattta ttccggttcg
     2341 gaaaaactta gattatcctt atgccgaaga agaccagcag agcagtagaa acactgtcca
     2401 gcaagcagga atggacattt ttgagcaacc atgcccatgt tctcatctgt ctctacagag
     2461 atgagaatgt ccgtcttaga gatctggctt tcaatgtagg cattacagaa agagcagtca
     2521 tccgcatcat agaggacctt gaggccgcaa atgtacttgt ccgattcaaa gaaggaagac
     2581 gcaataaata taaaatattt gataatattc ctcttagaca taaactggaa tcccacaaga
     2641 cgatcggaga tctcttaaag cttctcaaat aacagcatac cttctgcctc cggaccggtt
     2701 ctgcaggtct ggaaaccggc tctgaaactg cctaaaatcc cattttttcc gattcatcac
     2761 aaatttccaa gctgctcgat tttcctgatg ttttctgaaa attacagcgt atcacagctt
     2821 acgtgagatg ctcatttttc aaaggtcatt gcgcatctat tcattctcga ctcaatagaa
     2881 tcaggggttt cagcagggaa aaacgcaatg atttatgaaa atcagcataa attcgttctg
     2941 cgtaaaccag ttccgtaaaa ggtttctcat caaatttcga ttccgaaaca tggatactta
     3001 tttacagaaa attaatacat gaattatatt tcatgtattt aaagtattgc ttttaacttc
     3061 atataaaata atatggtatt tgcaaaaaga caggaggatt tatgaaaatc aaacatataa
     3121 gcgcagtttt ggctttttgt ctctgtgttc cggtttctgt tcaggcagaa atcagtccgg
     3181 atgaagcact gaacagactg acagaaggaa acagaagatt tatgcaggga aagtcgattc
     3241 atccgaatca gacaccggaa aagatcaaag agctttcttc aggacaaaga ccttttgcag
     3301 taattgtcgg atgctctgac tcaagggtcc caaatgagat tatttttgat cagggtctgg
     3361 gtgatctttt tattctgaga actgccggtc aggtttctac ttatgcgtcc tggggcagca
     3421 ttgagtttgc aaccgctgta ctcggtgcaa aactgattgt cgtattaggt catacaaaat
     3481 gcggagcagt cgcggcagcc tgcagtgttc cagatgttcc aggtcatatt gtcactctga
     3541 tcaatgcaat caaacccggg gtggaaatcg caagagaaat gaaaggcgat cttgttgaca
     3601 atgcagtaaa aatcaatgta gccagggagg tggaacagct gaaaaatctg gagcctgtgc
     3661 tttcgaagca gttcaagaaa ggagagatca gaattgtcgg ggctgtatac catttggaaa
     3721 cagggaaagt agaaatactt ccggataatt ttctaaaaaa ttttggagag gataaaaaat
     3781 aatggaaatg atagactctt taatgaatgg acttatgact cctatggtgc tggcattcgc
     3841 tctgggaata tttgccactt tgataaaaag tgatctgaag attccggaag ggctttatat
     3901 cggactcaca atttatctgc tgcttgccat tggaatcaag ggaggagtaa aactctcttc
     3961 caccccgatg gcagatttct taaaaccggc catgattgcg gttctgatgt gttctctgat
     4021 tccggtctgg tcatttttca tactgaacag aatgggaaaa ttcagtgctg cagattctgc
     4081 cgccgttgca gctcactacg ggtcagtatc cgtagtgact ttcagtgcag cccagacatt
     4141 tctcgaacag aagggtattt cattcgaagg atttatgcct acaatgcttg cgattatgga
     4201 aattccagga atcctggttg caatttttct ggcgagaatt catataaaaa atgataagtc
     4261 ttcttataaa aaactgtttc atgaactgtt tacaggaaaa ggaacagtcc ttctgatagg
     4321 aggacttatc ataggaatgc tcagcggaaa aaaaggattt gaacaggtct cgcctctatt
     4381 tgatactcca tttaaaggag tactgactct gtttcttctg gaagtaggaa tggtgacagg
     4441 aagaaaactg gaggatctta agaaagcagg agtttttctt ttctgttttg ccgttctaat
     4501 gcctctctta cattcttcca taggaatttc tttgggcaaa ttaatcggac tttctatggg
     4561 agggtcagtt attttcggag tgctatgtgc aagtgcatcc tacattgccg cccctgccgc
     4621 agtcagaatt gccatccctg aggcaaacac ttcgtattat ctgacagcat cactggcgat
     4681 tacatttccg ttcaacattg tgctcggatt tccgatctat acaaatattg caaaaatgat
     4741 ttatggagta aacacatgaa tttaagcatc gtacagttag tgacaatcat tgctcttgat
     4801 tcactggaag atacactgat ccaggattta aaagccctgg gaatgaaagg atatacagtc
     4861 agtgaagctc atggagaagg tctgagcaca agaagggatg acaactggga aggaagaaat
     4921 attaggatag aaattctggc cagacaggaa ctggcagaat ccattcttga gtttctgcag
     4981 aaagaatact ttccgaaata taagatgatt gcatttcttc aggaagtgaa ggtgcttcgg
     5041 aaggaaaaat ttcattgaca gcctcctgtc tcaaagtcaa atcattgaaa aatgagtgcc
     5101 tgctcagtaa aaagacattg caagaaagct tatagagaaa agcttaaaaa gcggggtctc
     5161 agccgcttta cagcttgcgg gggctgcaag gcctccgcga gaccggaaaa catctcccga
     5221 taagggactg cattctcctg ctcttttaaa tgcaatttct tgaaaaaagg atatttgcag
     5281 ccaggaaacc ttcaggatga gcagttacga aaaaataagg atgcaataca aatcatgaaa
     5341 catacttaca aattatttat tccttttatt tcagctgcat tgctcttttc cacctctgtt
     5401 ttttccggcg acggtgatat tcgggacaaa catgg
//
LOCUS       DAOQGJ010000028         5368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_7802_length_5368_cov_2.828534, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000028 DAOQGJ010000000
VERSION     DAOQGJ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7802_length_5368_cov_2.828534"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1770)
                     /locus_tag="PK683_00845"
     CDS             complement(214..1770)
                     /locus_tag="PK683_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07018.1"
                     /translation="MNWKTDESIYNFIVNSLDSEGKLKESGMELPDEIRTEGGLRYAP
                     GMADAVFGSTDTELSEEELAEISDLIFRAAVGDDECENKLYDNICHKYNVISIIDRVL
                     AKLDEDSFKLNGNLSIIAADFAFRSAERNAVKFGIALLGYCKSEDNLEEIKILGLHDE
                     FTLFASVAVMKISGNMERDLWEMAKKAEGWGRIQSVQRLADILCSPETEDWLVREGYK
                     NNIMYEYLTYIAAVHGKLYERLCAPKIDRGLFESAGEIIDGLISGGPAEDMSDYEFAS
                     RTVEQYIRHSVLMANELQDFIVLNSIKDFLEEIRDNLQGHEKNGWNQDNIADCLIDIN
                     TVMKKPEWKRKTEDALNSRLFAVFNQGKMTASILGIAYWDKLWALLKENPESTALWYD
                     AVNTAPPEFSDELTALALKTLPLDKIASGPADVVGFSEDYQYFICIEYIITYLGSFSG
                     KGEKLILAGLQAPVTRTRNMCVQTLGKWGRENWSEEIQLAVKKLSETEPNPRTKSNLE
                     RLLLGEKLVW"
     gene            complement(1911..2999)
                     /locus_tag="PK683_00850"
     CDS             complement(1911..2999)
                     /locus_tag="PK683_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07019.1"
                     /translation="MQTIWGGTAKKLKGNCLVIGAFISEGDNVWSVKEKKSTWAEVEK
                     AMNWLTKQAEEYEVDLSFELLPVNLEEESEDEAGDAIIDRLPRLADNAKKKNKAVNDA
                     CIELGYESVTDFYQSIIEGYADYSVHFLMLLNADDRSYMTTCPIDDEVKFLELNMVFK
                     NNGKINSFVVAHETLHAYSAMDLYNVAGDEAGKEAEEASKERCENEVMCAYQNDVENS
                     AVSDYTAFLIGWHDEPEDWYEEITQPFNRTKLKFIMNTYHHFDDDGNLSVETEEELLK
                     YSYEAGELTRIRLNEDDNLIQWRHYDNESEEEEEYWETGTDANYYYLDGKFIHTGITV
                     PKKSGQAYILYDVNQKYEPWVQMTQVES"
     gene            3105..3545
                     /locus_tag="PK683_00855"
     CDS             3105..3545
                     /locus_tag="PK683_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNH07020.1"
                     /translation="MKNILVAINFEDKESSVLLKKAVEISEKFGSKIWIVHIAMPNPE
                     FIGYDVGPQHVRDFRAKELKKEHKIILKHLDDLRAKNIECDGFLIQGAATDAILKESE
                     KLGIDLIILGHHKHGLLYKIFVNDVDGVIVNESKVPVLIIPLDK"
     gene            3547..4449
                     /locus_tag="PK683_00860"
     CDS             3547..4449
                     /locus_tag="PK683_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HNH07021.1"
                     /translation="MNPFHLNGPEFLAFYTGFGAVLLLISFIIRFLRENGDLPKVPLT
                     DPYLFSMLRAGEKETVRLAVGSLLHRNALENNGKNLLRSDAHITLEDNPLEKAVLHHF
                     SFEKDAFSVFTDPDVLAVCRKYENSLKSLQLLPDEQIKNSRMSVLLLSLTALVGLSGI
                     RIVRSIMNGHTNVLFLILLTVFFLVVFLLTSSPRQTVKGRRFLKDLQNTLNRDRTVSM
                     HPGTDSSLYLAAAVLGIGAFGTEFAFMQELYPKSYSNSWSSSWGSDSSSCGSSCSSGS
                     SCGSDGGGSGCGGGCGGGCGGGGD"
     gene            4412..5281
                     /locus_tag="PK683_00865"
     CDS             4412..5281
                     /locus_tag="PK683_00865"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNH07022.1"
                     /translation="MDAEAAVAAEGTEVRIGLGWRNEISSAILKHLDRIEILEVIIDD
                     YLDFSRKDIQLLKFISSQSPVTLHGIELGLASTIPIEEKRLARFAKLIHLIRPEYWSE
                     HIAFVRGLGVELHHLGAPPRNLNTFKGLLANLEKTEKIIGISPSMENPASPVDPPGSR
                     FTHTEWIKKISETVENRILLDLHNLYADSVNFGFDPKQFILSIPHGKIGTVHIAGGRR
                     IGNTVLDTHRHSVPEQVYELLEFTSSVHRNGFDVILERDGNYPEFSQILNELQRARES
                     VQRGRSIHELSEV"
BASE COUNT         1455 a         1273 c         1040 g         1600 t
ORIGIN      
        1 gatcaaagaa tcgctgtatc aagtaatggc ccctattttt atagaactca tcctgcatag
       61 ctgagctttc ggcaggaaat gtctgctgct ctgccttttc taagccgcag accaggatat
      121 cctgctccaa acagcacttc tgatgattca tagcatcatt atcaataaat tatattttta
      181 ttttcataat aagctctatt atactggcct gtactaccag accagttttt cgccaagaag
      241 aagtctttcc agattgctct ttgttcttgg attcggttca gtttcagaca gcttttttac
      301 tgccagctga atctcttcac tccagttttc cctgccccat tttcccaaag tctgcacgca
      361 catatttctt gttcttgtga caggagcctg aagtccggca agaatcagtt tctcgccttt
      421 ccccgaaaag ctccctaagt aagtaataat atattctata cagataaaat actgatagtc
      481 ttcactgaat cctactacat ctgcgggtcc ggaagctatc ttgtccaaag gcagtgtctt
      541 aagagccaat gcagtcagtt catccgaaaa ttccggcgga gctgtattca cggcatcata
      601 ccaaagagcc gtactttccg gattttcctt aagaagtgcc caaagcttgt cccagtaagc
      661 aattccaaga atggatgccg tcattttccc ctggttgaac acagcaaaaa gtcggctgtt
      721 cagtgcatcc tctgttttcc ttttccattc cggttttttc ataactgtgt ttatgtcgat
      781 aaggcagtct gcaatattgt cctgattcca gccatttttt tcatggccct gcaggttatc
      841 cctgatttcc tcaagaaaat cctttatgga attcaataca atgaaatcct gcagttcatt
      901 ggccataaga actgaatgcc tgatatactg ctcaactgtc ctggatgcaa attcataatc
      961 ggacatatct tctgccggcc cgccggatat cagcccatct ataatctctc ccgccgattc
     1021 aaagagtcct ctgtcgattt taggagcaca aagtctctca tacagttttc cgtgaactgc
     1081 tgcaatgtat gtcagatact catacattat attgttctta tatccctcac ggacaagcca
     1141 gtcttctgtt tcgggagagc acagaatatc ggccagcctc tgaacactct gtattctgcc
     1201 ccagccctct gctttttttg ccatttccca gagatccctt tccatattcc cggaaatttt
     1261 catcacagcc actgatgcaa acagagtaaa ttcgtcatga agtccaagta tttttatttc
     1321 ttccagatta tcttcacttt tacagtagcc taaaagcgcg attccgaact tcactgcatt
     1381 tctttcagca gaacggaaag caaaatccgc cgcaattata ctcaggttcc cgttcagttt
     1441 aaaactgtct tcatcaagtt ttgcaagaac tctgtcaata attgaaatga cattgtattt
     1501 atgacatata ttatcgtaca gcttattctc gcattcatca tctccgacag cagcacgaaa
     1561 tatcagatcg gaaatctcag caagttcttc ctccgaaagt tcagtatcag tgcttccaaa
     1621 aacagcatca gccattcccg gcgcatatcg aagcccccct tccgtcctta tttcgtccgg
     1681 cagttccatt cccgactctt tcaactttcc ttcagaatcc agactgttga ctataaaatt
     1741 atatatggat tcatctgttt tccagttcac aatcttttct ccatcagaga tgccggtcaa
     1801 aaatgaacca gcgccagatc gtcacggtaa ggaaaaaaac ctggacagac cgtcattctt
     1861 gggtgcaaac tctctgtatt tcgctcagaa tgactttatt atgcagtcat ctaagattcc
     1921 acctgtgtca tttgaaccca gggttcatac ttctggttta catcatacag aatataagct
     1981 tgtcctgatt ttttcggaac tgtaatgcct gtgtgaataa attttccatc cagatagtaa
     2041 taatttgcat ccgttccggt ttcccagtat tcttcctctt cctctgactc attgtcgtaa
     2101 tgcctccatt ggataagatt gtcatcttca ttcagccgaa ttcgcgtgag ttctccagct
     2161 tcataggaat atttgagcag ttcttcttcg gtctcgacgg aaagatttcc atcatcatca
     2221 aaatgatgat aagtattcat gataaatttc agctttgtcc tgttgaaagg ctgcgtgatt
     2281 tcctcatacc agtcttccgg ctcatcgtgc cacccaatca gaaatgcagt gtagtcggaa
     2341 actgcagaat tctccacatc gttctggtag gcacacatca cttcattttc acaccgctct
     2401 tttgaagctt cttctgcctc ttttccggcc tcatccccgg cgacattgta aaggtccatt
     2461 gcactgtagg cgtgaagagt ctcatgtgcc acgacgaagg aattgatttt cccgttgttc
     2521 ttgaaaacca tattgagctc gagaaacttt acttcatcat caatcggaca agtggtcata
     2581 taggatctgt catctgcatt cagcagcatc agaaaatgaa cgctgtaatc cgcataaccc
     2641 tcaattatac tctgataaaa gtcggtcact gactcatagc caagttcaat acaggcatca
     2701 ttcacggcct tgtttttttt cttcgcatta tcagccagac gcggaagtct gtcaataatg
     2761 gcatctcctg cctcatcttc gctttcttct tcgagattga ccggaagcag ttcgaaactg
     2821 agatccacct catactcttc ggcctgcttc gtcagccagt tcatcgcttt ttccacctcc
     2881 gcccaggtgg attttttttc cttcactgac cacacattat cgccttctga aatgaaagca
     2941 ccgatcacaa ggcaattgcc tttgagtttt tttgctgttc cgccccaaat tgtctgcata
     3001 atggctgatt atatacgtct cttttaacag tcaagcagtg aatttgagga aaagcagttg
     3061 actctaaatg agcaggattt ttgatttagg aaggaaggat tctcatgaaa aatatactcg
     3121 tagccatcaa ttttgaagac aaggagtcct ctgttcttct gaaaaaagct gtcgaaattt
     3181 ccgaaaaatt cggctccaaa atctggattg ttcatattgc aatgcccaat cccgaattca
     3241 tcggttatga tgtcggtcct cagcacgtca gggatttcag ggcaaaggaa ctgaaaaaag
     3301 agcacaaaat catcctcaag catctggatg atctgagagc aaaaaatatt gagtgtgacg
     3361 gattcctgat tcagggagct gctaccgatg caattcttaa agagtccgaa aaactgggga
     3421 ttgatctgat tatcctcggt catcataagc atggcttatt atataaaatt tttgtaaatg
     3481 atgtggacgg ggtgatcgta aacgaatcca aggttccagt tctcatcatc cctctggaca
     3541 aatgaaatga atccgtttca tctgaacggt cccgagtttc ttgcttttta cacagggttc
     3601 ggggcagttc tgcttctaat ctcctttatt atcagattcc tgagagaaaa tggggacctg
     3661 cccaaggttc ctctgactga tccctatcta ttcagtatgc ttcgcgcggg agaaaaagaa
     3721 acagtccgtc tggcagtggg ttcgcttctg cacaggaacg cactggaaaa taatggaaaa
     3781 aaccttctcc ggagcgatgc tcatattaca ctagaagaca accctttgga aaaagcagtc
     3841 cttcatcatt tctcttttga aaaggatgca ttcagtgtgt tcactgatcc tgatgtgctt
     3901 gctgtatgca gaaaatatga aaattcatta aaaagtcttc agctccttcc ggacgaacag
     3961 ataaagaaca gcagaatgtc cgttcttctc ctttcactga cagccctggt cggactttcc
     4021 ggcataagaa ttgtgagaag cattatgaat gggcatacaa atgtactttt cctgattctt
     4081 cttacagtat tctttcttgt tgtttttctt ctgacctcaa gtccaagaca gacagtaaaa
     4141 ggcagaagat ttctaaaaga tcttcagaat accttgaaca gagacagaac tgtctctatg
     4201 catcctggaa cagattcgag tctttacctg gctgccgctg ttctcggcat cggtgcattc
     4261 gggacagaat ttgcatttat gcaggaactc tatccgaaat cctattccaa ctcctggagt
     4321 tcttcctggg gaagcgactc ttcttcctgc gggtcgtcct gcagttccgg atcctcctgc
     4381 ggttccgacg gcggcggaag cggatgcggg ggtggatgcg gaggcggctg tggcggcgga
     4441 ggggactgaa gtcagaatcg gactcggctg gagaaatgaa atctcttcag ccattctgaa
     4501 acatctggac agaatagaaa tactggaagt catcatcgat gactatctgg atttctccag
     4561 aaaagatatc cagcttctaa aatttatttc ctcacagtct ccggtcacgc tgcacggaat
     4621 agagctggga ctggcttcca caattccaat tgaagagaag agactcgcaa gatttgcaaa
     4681 actcattcat ctgatacgcc cggaatactg gtcggaacat atcgcatttg tcaggggtct
     4741 cggagtggag ctccatcatc tgggcgcacc tcccagaaat ctgaatacat tcaaaggact
     4801 tcttgcaaat ctggagaaga cagaaaagat catcgggatt tctccgagta tggaaaatcc
     4861 tgcttctcca gtcgatcctc ccggcagccg ttttactcat acagaatgga ttaagaaaat
     4921 ttcagaaaca gtggaaaaca gaattcttct ggatcttcac aacctttatg cagactcggt
     4981 aaatttcgga tttgatccta agcaatttat attatctatt ccacatggta aaataggaac
     5041 agtgcatatt gcaggaggaa gaaggatcgg gaatactgtt ctcgacaccc acagacattc
     5101 tgttcctgag caggtgtatg aacttctgga atttacatcc tcagttcaca gaaacggttt
     5161 tgatgtgatt ctggaaagag acggaaatta tccggaattt tcacagattc tgaatgaact
     5221 tcagcgtgcc agagaatcag ttcaaagagg cagatcaatc catgagctct ccgaagtttg
     5281 aaacattcct cgcacttctt tacacggatg cataaaaatg aaaactctta agttatataa
     5341 tttttaatta cagataaaac atcattca
//
LOCUS       DAOQGJ010000029         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8017_length_5274_cov_3.808392, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000029 DAOQGJ010000000
VERSION     DAOQGJ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8017_length_5274_cov_3.808392"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PK683_00870"
     CDS             complement(<1..959)
                     /locus_tag="PK683_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07023.1"
                     /translation="MDLSVNIKRKNVKEHIKKYTVLIIDDEKANLDALTSLLESEYYI
                     IQAENGQEALSVIKESDKEIHIIIADQRMPGMKGVEFFRQSIQLLPKAVRILMTGYTD
                     TSEIIDAINEGHIYKFITKPADMQNILQTVRRSAEFWETENENRRLIRELEEKNQYIA
                     ESRKLYEQLFLNAPIGILLLDTAGAIHSVNPAALEILGSKSEEDTKKFNCLSFSPLIE
                     AGIADLIENCIESRSLVKSEVFYKSFWGKELYLFCIFSYIKGENEKDWIYAIIKDITE
                     RKFIQLQLEKLIERTKQAMSANKMKSEFLANMSHEIRTPLNAVT"
     gene            complement(1091..2788)
                     /locus_tag="PK683_00875"
     CDS             complement(1091..2788)
                     /locus_tag="PK683_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07024.1"
                     /translation="MNKDFLTWDPELRYVLTLKMMNHLRNGELHLFLLQFFDSPHWPS
                     LRNVFQSLIFLITSHSPYIDVLLSFIFLPLSYLSLVSLLILYRIPLLASSVYCITYTL
                     LLLQADPILLYSLTGMLEVQGMFVFLWVLYYLAKVYTEKEFIQFSRNGWKLFGISFLL
                     YQTKYPYGYMLILSVILFHGIFFFRETLNFALDYLFIHLRYFFRNRLLILSFLLFISS
                     ILLPKTVLAGKTAGYIRYAAVLFFVIDFFMFFKSLSYTEERARIQFLLKWIFFPIVFW
                     MLIQPDRFGSYSGQITHVESQGFNPGEKIIKDWDYYFIFFSEFLINGFRTWKISYMLL
                     VTAVFAFVYGAWNGFSKKKYSSSFLISAITLITLAELSLFTTNRLARHTYHLFGVILL
                     IPAVLHLEISSVRGLRPAAYALTLLFFFAALDSFQFNPAKNLQNTEICYTGGNKADYY
                     TPRHIEAQALLYLNSDAVILNEVNPLHVNKADTEYLLSRIAYDRRITLQFDPKRMKKL
                     PSSVKEFWVVSEKKRESEKKSKMILFLNSLSIELEEPVILEDDWSYIEILRFKQRKN"
     gene            complement(3797..4117)
                     /gene="cutA"
                     /locus_tag="PK683_00880"
     CDS             complement(3797..4117)
                     /gene="cutA"
                     /locus_tag="PK683_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNH07025.1"
                     /translation="MKEETGMAYITCSSIEEAMKMGEILVKDRIAACANIIPKMISLY
                     IWKNELEKSEEAVLITKILKKNYAKLETRVKELHSYTVPCILYYPSSAGYPPYIQWMI
                     ENSA"
     gene            complement(4311..5246)
                     /locus_tag="PK683_00885"
     CDS             complement(4311..5246)
                     /locus_tag="PK683_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNH07026.1"
                     /translation="MAGFSDSPYRRLTREFGSAFSFTEFVSADMISHHSKKSIDLFRF
                     HPSERPIWFQIFGNSKHTISEAVKIITDLEPDVIDLNMGCSTCKVSNRGSGAGLLKNL
                     KYAGEIIEEMRRSTHLPVTAKIRIGWDHDSLNYKETVHILQESGIQMISVHGRTKSMG
                     YSGKADWNIIAEVKSIAKIPVLGNGDITDPVLAQEKRKFSNVDGVLIGRGALGNPWIF
                     SGIKKTDLDFRQVRDVMKRHLHLMLDFYGEDQGMILFRKHTSKYLAEYSDILEQKKKL
                     LTSQSLNEYLDILEELNPNPSNIIQSEADEPLECT"
BASE COUNT         1672 a         1006 c          972 g         1624 t
ORIGIN      
        1 gtaacagcat tcagaggagt tctgatctca tgactcatat ttgcaagaaa ctctgatttc
       61 attttgtttg cagacatagc ctgctttgtt ctttcaatca gtttttccag ctgaagctga
      121 atgaattttc tttcagtgat atcctttata attgcgtaaa tccagtcttt ttcattttct
      181 cctttgatat aagaaaaaat acagaataaa tacagctctt ttccccagaa cgacttgtaa
      241 aacacctctg attttacaag tgacctgctt tctatgcagt tttcgatcag atcggcaatc
      301 cctgcctcaa ttaaagggga aaaggaaaga cagttgaatt tttttgtatc ctcttctgat
      361 tttgaaccga ggatctccag cgcagccgga ttgactgaat gaattgctcc tgcagtatcc
      421 agaagaagga ttcctatcgg cgcattcaga aaaagctgtt catacagctt gcggctttca
      481 gcaatatact gattcttttc ttccagttcc cggatcagcc ttctgttttc attttcagtt
      541 tcccaaaact ccgcagatct tctgaccgtc tgaagaatat tctgcatatc ggctggtttt
      601 gtaatgaatt tgtatatatg tccttcattg atggcatcga tgatttcaga agtatcggta
      661 taccctgtca tcagaattcg gactgctttg ggaagcagct gaatggattg tctgaaaaat
      721 tcgacgcctt tcatcccggg cattctctga tcggctatga tgatatgaat ctctttatct
      781 gattctttaa tgacagacag tgcttcctgt ccgttttctg cctgtattat ataatattca
      841 gattcaagaa gagaagttag agcatccaga ttggcttttt catcgtcgat gataagtaca
      901 gtatactttt tgatatgttc ctttacgttt ttccgtttta tgttgaccga cagatccata
      961 tcttttatga aagtattccg tataattcac acagtcgtca ggatactgca ctgccgcctc
     1021 actgtttttg cttcttttta tatctatatt gcgcagttat aaaaaaagca agcaaagctt
     1081 tctgccaatt ttagtttttt ctttgtttaa accgcaagat ctcaatataa gaccaatcat
     1141 cttccagaat tacaggttct tccagttcta tcgaaagcga attcagaaag agaatcattt
     1201 tcgatttttt ttcagactct cgtttctttt cagacacaac ccagaattct tttacagaag
     1261 atggcaattt cttcatccgt ttcggatcaa actgaagagt gattcttctg tcataggcaa
     1321 tcctggagag aagatactcg gtatcagctt tgtttacatg aagaggattt acttcattca
     1381 gtatgacagc atctgaattc agatacagca gtgcctgtgc ttctatatgt ctgggtgtat
     1441 aatagtcagc cttgtttcct ccagtgtaac agatctctgt gttctgaaga ttttttgccg
     1501 gattaaactg aaatgaatcc agtgcagcaa agaaaaaaag aagcgtaagc gcataagctg
     1561 caggtcgaag tcctctgact gatgatattt ccagatgcag aacagcagga atgaggagaa
     1621 tcactccaaa aagatgatat gtgtgccttg caagtctgtt tgtagtaaac agactgagtt
     1681 cagcaagagt aatcagtgtg attgcagaaa tcagaaagga agaactgtat ttctttttag
     1741 aaaatccgtt ccaagctcca taaacaaatg caaaaacagc agtgacaaga agcatatagg
     1801 atatcttcca ggttctgaat ccgttgatca gaaattccga gaagaaaata aaataatagt
     1861 cccagtcttt gatgattttt tcacctggat tgaagccctg tgattccaca tgagtgatct
     1921 gacctgaata acttccgaat ctgtcaggct gaatcagcat ccagaataca atcggaaaga
     1981 atatccattt cagaaggaac tgtattcgag ctctctcttc agtataggaa agtgatttga
     2041 agaacataaa aaaatcaata acaaagaaaa gaacagctgc ataacgtatg tatccggctg
     2101 tttttccagc cagaacagtc tttggaagca gaatggaaga gataaagagc agaaatgaaa
     2161 gaatcagcag tctgtttctg aaaaagtatc tgagatgaat gaacagatag tcaagagcaa
     2221 agttcagcgt ctctctgaaa aaaaatattc catgaaacag aatcacagaa agaataagca
     2281 tatatccgta aggatacttg gtctgataca aaaggaagct gattccaaag agtttccagc
     2341 cgtttctgct gaactggatg aactcttttt ctgtataaac ctttgcaaga tagtataaaa
     2401 cccacaggaa tacaaacatt ccctgcactt caagcatacc agtcaatgaa taaaggagaa
     2461 ttggatcagc ctgaagaaga agaagcgtat aggtaatgca gtaaacagaa gaagccagaa
     2521 gaggaatcct gtataaaatg agaagagata caagacttaa gtatgataaa ggaaggaaaa
     2581 taaaggatag aagcacatca atgtatggag aatgagatgt gataagaaaa atcagcgact
     2641 gaaatacgtt tctaagagac ggccagtgcg gagaatcaaa gaattgaaga agaaaaagat
     2701 ggagttcacc gtttctgaga tgattcatca tctttaaagt aagaacataa cgcagttccg
     2761 ggtcccatgt aagaaaatct ttgttcacac atcggagcaa atattcattc agcactgtat
     2821 tcagatgaaa aaataatagg aaggcagatg aagcaagggc aataaagaat attataaagg
     2881 atgattgagc gtaaatcttt ctgttcactc tcaaaacaag ctatgctata ttctttattg
     2941 caagaatttt tcattctcat cgtatgattt tactcttatg atcgtttatc tgaatttttt
     3001 ttcagaaaag ataaaaaaat ttatgcgaat ataataaaag gctttacagg aaaatatacg
     3061 gatgtaagtt atgtcacaaa taaacaaacg catttgagat acaaaagaaa acaaatgagt
     3121 ttaaaactaa ataggaggct ctcaatggtt gctaaaaaga aagctgcttc aaaaaagaaa
     3181 tctgcatcca agaagaaatc tgcttctaaa aagaagtaat ttcggtcttg aaacaaagta
     3241 aatgaaattt taaaacccgc tcacaaggcg ggttttttgt ttttattgtc tcttatttaa
     3301 aaatatcatt cacgctgatc cgcattcaat gcattcttcc ataaattcat atctttctgc
     3361 agtcctgctg ctgaatataa atcaatgaat tcagaccttt cgagcattcc tgcttataat
     3421 ctcacagact ttgacctcag aacgcttaca gaggctttta ttccgttatt tcaggcatta
     3481 aaaaacagtt tatgcagtca aagcagtcaa tcgtaaaaca aaaaacactg gtcacaacaa
     3541 ccattcaatc taagttcaat gatttaactg cttaaactga cagtaaacgc atagaaaaga
     3601 acctcatagg gctggtcttt ttcaatactg tctctatgtc tgacggttca ttctcagata
     3661 aatgaagcaa gctgtccttc aaaatatgaa ctgaagatat atttgataaa aaaagttcca
     3721 tcattgagca gataagatga tgattattaa aacagaaaca cgaatataca cagaagaaag
     3781 cagtcaactc ctgtatttat gcagaatttt cgatcatcca ttgaatgtaa ggaggatatc
     3841 ctgctgatga tgggtagtat aatatgcagg gaacagtata actgtgaagt tctttcactc
     3901 ttgtctcaag ctttgcatag ttttttttca gtatttttgt tataagaacc gcttcttcag
     3961 atttttcgag ttcattcttc cagatataga gtgaaatcat tttaggaatg atgtttgcac
     4021 aggccgcaat cctatccttt acaagaattt ctcccatctt cattgcttct tctatgctgc
     4081 tgcaggtaat ataagccatt cctgtttctt ctttcataat gaacagctca gttttgcttg
     4141 aatttatttc aattctttat tcaatttatg acagtcagga gcagcagaca ttgtccaatg
     4201 ctttcagtta agagtatcat aaccttgcga aacaaggtat cacagaagtc acttaatgta
     4261 tgatgagaat atatgtaaga attttcttca gaatctatgg atgtctgtgc ttacgtgcac
     4321 tcaaggggtt catcagcttc tgattgaatg atatttgatg gatttggatt caattcctcc
     4381 agtatatcca gatattcgtt caggctttga gaagtaagga gcttcttttt ctgttcaagt
     4441 atatcggaat actctgccag atacttcgaa gtatgctttc tgaacaggat cattccctga
     4501 tcttcaccgt aaaagtccag cataagatgc agatgccttt tcattacatc cctgacctgc
     4561 cgaaaatcca aatccgtttt ctttatcccg gaaaatatcc aaggattgcc caatgcacct
     4621 cttcctatca gcaccccatc cacattggaa aactttcttt tttcctgcgc aagaactgga
     4681 tcagtaatat caccgttgcc taaaacagga atctttgcga tggatttcac ttctgcaatg
     4741 atgttccagt ccgctttccc ggaatatccc attgattttg ttcttccatg aacggaaatc
     4801 atctgtattc ctgactcctg caggatatga acagtctctt tatagttcaa tgaatcatgg
     4861 tcccagccta tcctgatttt tgcagtgact ggaagatgag tggaacgtct catctcttct
     4921 ataatttctc ccgcatactt cagatttttc agaagacctg ctccgcttcc cctgttggat
     4981 accttgcagg tggaacatcc catattcaga tcaattacat ccggttccaa atctgtgatg
     5041 attttaaccg cttcagagat cgtatgcttg ctgtttccga agatttggaa ccagatcggc
     5101 ctttcagagg gatgaaaacg gaaaagatca atggattttt tggaatgatg agaaatcata
     5161 tcggcactta caaactcagt gaatgaaaat gccgagccga attctcttgt cagtctcctg
     5221 tatggactgt cggaaaaacc ggccatcgga gaaagaattg catctccttc aata
//
LOCUS       DAOQGJ010000030         5254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8065_length_5254_cov_4.202347, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000030 DAOQGJ010000000
VERSION     DAOQGJ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8065_length_5254_cov_4.202347"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..805
                     /locus_tag="PK683_00890"
     CDS             185..805
                     /locus_tag="PK683_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNH07027.1"
                     /translation="MKSKKTAVKNTKSVKKHDPFIHKSATVIGMVKMGKDCSIWPGAV
                     LRGDTKEIILGNAVNIQDNCTLHTDSQNPIRIGDYTLVGHNAILHGCTVGKGCLIGIG
                     SIILDKAEIGDGAMITAGCMIRGGMKIPSRAMVVQKDGKLQIYENKAKVQLTIAGCLE
                     YIALAERHRKKIFKPFTPAEEKHFIDRAEEIMKDLGLDSFAVPAIE"
     gene            complement(795..1607)
                     /locus_tag="PK683_00895"
     CDS             complement(795..1607)
                     /locus_tag="PK683_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07028.1"
                     /translation="MRFFLKISFLLLSLSLYSSDRLPDPMPDDIVIKYYKPLGHAGSG
                     FSMELTRKGCRYEDTKIQKSNGFDFSLSEDELKAVYRILQENKIDRIGYTDGLIHDYD
                     GVSLSVHWKTGNAEVSQSGRKVSSRWLNEWKTVTDVLEKIREKEYSKALQEYSVFIDQ
                     SLLGNFIHIQSDTGLSLRKYINQSVLKENPIRAKTLPGTHSVYCILYQGYDLKKRNEI
                     YSDFDPERASLWDEKHNAYINTLKTISFSLKLDTSSKKSFRIMKKGDQIIIQ"
     gene            complement(1621..1995)
                     /locus_tag="PK683_00900"
     CDS             complement(1621..1995)
                     /locus_tag="PK683_00900"
                     /inference="COORDINATES: protein motif:HMM:NF008740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HNH07029.1"
                     /translation="MSLLGNIIWLICGGFLSGLGYILGGIVTCLTIVGIPFGWPTIKI
                     GFATMAPFGKENVVTDSSSGLLRMIFNVLWLVFFGWEIALAHLAHAGILAITVVGIPF
                     AVQHMKLIPMALFPFGRELRKK"
     gene            2596..4488
                     /locus_tag="PK683_00905"
     CDS             2596..4488
                     /locus_tag="PK683_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNH07030.1"
                     /translation="MIKNTLLDVFEKASRNRDQAFYTKDRFKFFRAESFTSLYEKAQT
                     LALFLMDKGIKKGDRVSVFSDNRAEWMIADMAILLAGAVTVPRGSDSTETEIEFILNH
                     SESSLVFIEHQRLYKKISRVLSEKKIPVIVLDSAFPKEIISQNNFFSIHDIVQNRSAL
                     VPRLSELKKVRDSVTGDDLFTIIYTSGTTGNPKGVMLTHSNMMYQLKIVPQVLDISQT
                     DRILSILPVWHIFERFMHYSVIDSGACCYYTNIKDLMEDFIRVKPTLMASAPRLWEQV
                     YHKLHERIEKTEALNRELFNISYGIKQSLHRAWNIFTDNTAETRKKNFLESLTDKIGA
                     LIKTAVLKLPDMYMDPIFLVRVRAMLGGELKGTLSGGGALPVHIDEFFNAIGIPVYEG
                     YGMTECSPLISLRPKGRIIEGTVGFTPEGTQVRLMKEDGTAAAQGEQGVIFVKGPGVM
                     KGYYKNPDTTALVLNDGWMNTGDIGVVSVNGALSIRGRAKDTIVLLSGENVEPVPIET
                     ILTQHVMIEQAVVVGQDRKHLGVLIWPNYEKLEDAGYAVSEHDKSLDLNQHKEIRHLF
                     LSIVHRLIGEENGFKSFERVTDLRFLPDKLRVGETLTNLQKIKRNVVFSKYENLIQSM
                     YGDHHL"
     gene            4485..>5254
                     /locus_tag="PK683_00910"
     CDS             4485..>5254
                     /locus_tag="PK683_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH07031.1"
                     /translation="MKTKILLRTPDHWNVALYKYHCPKVTKKYPVFLLHGIASNHTVW
                     DTGYSQYDFSRKLTDEGYQVYALDLRGRPGSDGPHTGRGSMWSIDDYLLCDLPAAIEY
                     IIEDFGSEKIHWVGHSLGGILGFFYQIRHKASNLQSLTAFASALTYTFSTINHFRTYM
                     DYISALMYYPVDLWYKIVYPVIDMDTYFTRFIWNADNLAPEVKRAIIENTVQKIAVLE
                     WNQIKTISAAEGMPRISGGFNHYVDDRRIVTPAFLITG"
BASE COUNT         1595 a         1077 c         1144 g         1438 t
ORIGIN      
        1 caggcagatg cgccggacat taaaaaaaac agaaccttgg tgtattttga caatgcccat
       61 tcgcgcattc ttctctccga aaattcagat aaagcacctg cacagacctg tctgactgtc
      121 agcctgaaat attcagagaa gagagcttcc tgcagaaaag attggacata aaaaaggaga
      181 acttatgaaa tcaaagaaaa cggcagtaaa gaatacaaaa tcagtaaaaa agcatgatcc
      241 cttcattcac aaatcagcga ctgtaatcgg aatggtgaaa atgggaaagg actgttccat
      301 ctggcccggt gcagttctca ggggagacac taaagaaatt attttaggaa acgcagtgaa
      361 cattcaggac aactgcactc ttcatacgga ttctcagaac ccgatcagga tcggagacta
      421 tacactggta gggcacaatg caattctgca cggatgcaca gtcggaaaag gatgtctgat
      481 cggaatagga agcatcatcc tggacaaggc tgaaattggc gacggtgcaa tgattactgc
      541 gggatgcatg attcgaggcg gaatgaaaat tccatccagg gcaatggttg ttcagaagga
      601 cggaaaactg cagatatacg aaaacaaggc aaaggttcag ctgacaatag caggctgcct
      661 ggaatacatt gctcttgcag agcgtcacag aaagaaaata ttcaagccct tcactccggc
      721 agaagaaaaa catttcattg accgagcgga agagattatg aaagatctgg gacttgacag
      781 ttttgctgtt ccggctattg aataatgatc tgatctcctt ttttcattat ccggaatgat
      841 tttttggaag atgtgtccag cttgaggctg aatgagattg tcttcagtgt attgatgtat
      901 gcattgtgct tttcgtccca gagggaagcc ctttccggat caaagtctga gtagatctca
      961 tttctttttt tgagatcata tccttgataa agaatgcagt atacggaatg tgttcccggt
     1021 aaggtctttg cacggatagg attttccttc agaactgact gatttatata tttgcgaaga
     1081 gagagtcctg tatcagactg tatgtgaatg aaatttccca agaggctctg gtcaataaaa
     1141 acactgtatt cctgaagcgc cttggaatat tctttttctc tgattttttc gagaacatcg
     1201 gtgacagtct tccactcatt caaccatctg gagctcacct ttcttccgct ctgtgaaact
     1261 tccgcatttc cagtcttcca atgcacagaa agggatacac cgtcataatc gtgaatcaga
     1321 ccgtctgtgt aacctatcct gtcaattttg ttttcctgaa gaattctgta gacagccttc
     1381 agttcatctt ccgaaagact gaaatcaaat ccgtttgatt tttgaatttt cgtgtcttca
     1441 taccggcatc cttttcgcgt cagctccatt gaaaaaccgg accctgcatg tcctaagggt
     1501 ttatagtatt ttattacaat atcatcaggc ataggatcgg gaagtctgtc ggaagaatac
     1561 aaagataatg ataataataa aaatgatatt tttagaaaaa atctcatatc atccgtattc
     1621 ttactttttc ctgagttccc ttccgaatgg gaaaagtgcc ataggaatca gtttcatatg
     1681 ctgaaccgca aagggaattc ccaccacggt gatcgcaaga atgccggcat gagcaagatg
     1741 agcaagggca atctcccatc cgaaaaaaac cagccagagg acattaaata tcatcctcag
     1801 cagtcctgaa gaggaatcag tgacaacatt ttcctttccg aaaggagcca tagttgcaaa
     1861 gccgatttta atcgtaggcc atccgaaagg aattcccaca attgtaagac aggtgacaat
     1921 cccgcccagt atataaccga gaccggacag aaagcctccg cagatcagcc agataatatt
     1981 cccaagtaaa ctcataaatc tcctcataaa caaaaaataa atatacttat aaactgtatt
     2041 ctttataaat actcagattt ctaatctcgc tacgctccgc ccttcgggct attagaaatc
     2101 aaaaatggta gggatgccta ccggcattgg tttaaagggg cttaagcagc ccctttaaaa
     2161 tccccgccct tgctcacatt cagtgtcgac cagaactgat tttaaaccaa tatttcattg
     2221 atcctattat aatctaatta gaaatctggg tattaagttt acagatcatt gaatatgagt
     2281 aactcacctt atgcctgccc tccctaatgc tgtacaattg acaatacacc gctttgcgta
     2341 acgtgagtga gtaaaaggtg agggtttcag taggtgttca gtcttggctt ccaattctgc
     2401 gaacgggttt ttttaagccc cttagatgct tccggatttg tgaatcgggc aagtcgagaa
     2461 attgcataag tattctttat tcggcttttg ggacgtacaa ataagtctgt cttgctgtaa
     2521 atgaattgtc ttctcactgc atattttgaa tcttggatga aaatcatcca acggatgcac
     2581 ccggagaaat gcagaatgat aaaaaatact ctgcttgatg tatttgaaaa ggcctcgcga
     2641 aacagggatc aggcatttta taccaaagac agattcaagt ttttcagagc ggaatccttc
     2701 acctctcttt atgagaaggc tcagaccctg gcacttttcc tgatggacaa aggaattaaa
     2761 aagggagaca gggtctccgt tttttccgac aacagggcag aatggatgat cgctgatatg
     2821 gcaatccttc tcgctggtgc agtcactgtt cccagaggtt ctgattccac tgagaccgaa
     2881 atagaattca tactgaatca cagtgaatcc tctcttgttt tcatcgaaca tcagagactt
     2941 tataaaaaaa tttccagagt tctttctgaa aagaaaatcc ccgtcattgt tctggacagt
     3001 gcatttccta aagagattat atcacagaat aattttttca gcatacatga tattgtgcag
     3061 aacagatctg ctcttgtccc aagactaagt gagctgaaaa aggtgagaga ttctgtcacc
     3121 ggggatgacc ttttcacaat catttataca tcaggaacaa caggtaatcc gaaaggagtg
     3181 atgctgactc attcgaatat gatgtatcag ctgaaaattg ttccccaggt tctggacatc
     3241 agtcagacgg acagaattct ttccatcctc cctgtctggc atatatttga aagatttatg
     3301 cattattccg taattgattc cggagcctgc tgctattata caaacatcaa ggatctgatg
     3361 gaagacttta tccgtgtaaa accgactctg atggcatctg ctccccgtct ctgggaacag
     3421 gtataccata aactgcacga aagaatcgaa aaaactgagg cactgaacag ggagcttttt
     3481 aatatatctt acggaatcaa gcagagtctt cacagggcat ggaatatttt tacggacaat
     3541 actgccgaga ctagaaagaa aaattttctg gaatctctga ctgataaaat aggtgcactt
     3601 atcaagactg cggtactgaa acttccggat atgtatatgg atcctatttt ccttgtccga
     3661 gtgagagcga tgctgggagg agagctgaag ggaacactct ccgggggagg tgcacttcct
     3721 gtgcatatag acgaattctt caatgctatc ggaattcctg tttacgaggg atacggaatg
     3781 acagaatgtt ctcctctcat ctcactccgt cctaaaggaa gaatcattga aggaacagta
     3841 ggattcactc cggagggaac acaggtcaga ctgatgaaag aggacgggac cgctgcagcc
     3901 cagggagagc agggagtcat tttcgtaaaa ggtccaggtg taatgaaggg atattataag
     3961 aatcctgata ctacagctct tgtactgaat gacggatgga tgaataccgg tgacattggt
     4021 gttgtttcag tcaatggagc attgagcatc agaggacggg caaaggatac tattgtcctt
     4081 ctcagcgggg aaaacgtaga gccggttccg attgaaacca ttctcactca gcacgtaatg
     4141 attgaacagg ctgttgtagt gggccaggac aggaagcatc tgggagtgct tatctggccg
     4201 aattatgaaa aactggaaga tgcaggatat gcagtttccg aacacgacaa gtctcttgat
     4261 ctgaatcagc acaaggaaat caggcatctt ttcctttcaa ttgtgcacag gctgatcggt
     4321 gaagagaacg ggttcaagag ttttgagcgt gtcacggatc tgagattcct tccggacaaa
     4381 ctcagagtag gagagactct cacaaatctt cagaaaatta agcggaacgt tgtgttcagc
     4441 aaatatgaga acctgattca atctatgtac ggagatcatc acttatgaaa acaaaaattc
     4501 ttctcaggac tcctgatcac tggaatgtag cactttataa atatcactgt ccaaaagtca
     4561 caaaaaaata tcctgtgttt cttcttcatg gaatagcatc caatcataca gtatgggaca
     4621 ctggatattc tcagtatgat ttttcccgga agcttacaga tgaggggtac caggtatatg
     4681 cacttgatct gcggggcaga ccgggaagcg acggtccaca taccggaagg ggaagcatgt
     4741 ggtcgattga tgattatctg ctctgtgatc ttcctgctgc aatagaatat atcatagaag
     4801 atttcggatc agaaaaaatt cactgggtgg gtcattccct cggcggaatt ctgggttttt
     4861 tctatcagat cagacataag gcatccaatc ttcagagtct gacagcattt gcatccgcac
     4921 ttacatatac attttccaca atcaatcatt tcagaactta tatggactat ataagtgcgc
     4981 ttatgtatta tcctgtggat ctctggtaca aaattgtata tcccgtgata gatatggata
     5041 cttattttac acggtttata tggaatgctg acaatctggc tcctgaagta aaaagggcca
     5101 tcattgaaaa tactgtgcag aaaattgcag tcttggaatg gaatcagata aagactattt
     5161 ctgcagctga aggaatgccc agaatctccg gcggtttcaa tcattatgtt gacgaccgac
     5221 ggatagtcac cccagcattt ctgattacag gaga
//
LOCUS       DAOQGJ010000031         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8073_length_5251_cov_2.871632, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000031 DAOQGJ010000000
VERSION     DAOQGJ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8073_length_5251_cov_2.871632"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK683_00915"
     CDS             <1..258
                     /locus_tag="PK683_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07032.1"
                     /translation="LNSRPEDLLPLFLELNEEISAEEILVIKFSELQDLTFSMASMVS
                     SFILLVENSHKTAVSIQAEPELSKKLKEANFESIVSKFMPS"
     gene            270..2810
                     /locus_tag="PK683_00920"
     CDS             270..2810
                     /locus_tag="PK683_00920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNH07033.1"
                     /translation="MAGIDYNELKESFVNSSAAILQRSESCILILESSVDKNAIDELF
                     RGIHTIKGNSGIFELKNIPRLSHSFENVLNLLRTSRLMPTPDLIDLMLLGIDRLREMI
                     KDLKASESVDADDLIEKFNAYLSPAKDKEKASKDSKTVSEMQISNEETKWMKHIKVSD
                     KIIEFAKSKKKYLSIVIFSLEVQQFRTVSGLLEKIQMIAKKARVLRKGLITKKLTPLE
                     NKINLHLPYFLILLSDSKVEEFLEEIGIQPEFVRTVFVPDNAESVHINPDRQNTEQTQ
                     TAKQVHPIKEISVKDTSINVNLDLLNHLINLTGEIVLARNILSLKILQSGNSELQSIS
                     KRFENLLSELESGILKTRLQSMNVFFQKIPRLVRDISKATGKQIDVHIEGGDVELDKS
                     LIDEVSDPVTHLIRNAIDHGIENSEERQKKGKNSVGNLRVSARLRGGNVELIIGDDGR
                     GLDIVKIRERIIQKNLAPPELVHRIPKEEIIEYLFMPGFSTAGSVTELSGRGVGLDVV
                     RSNVKRIGGTVSIEFEENSGTSFILSIPQTLSIINCLVFKSGNRKFAIPQQYLCELIK
                     INPINITKIENKSLYYLRNQMLPLVNLKNVFHIENDELKQSVEKFIIALKSEKYYFGI
                     NVDEILDTEEIVVKPLGEYFEELPFFSGVTVLGDGDIALILDVSGLARLSGLQNSQNL
                     IESSALKEISKAEEIYLLFSVGAVKVTVSIASNPYIIKISAKEIIHFLGLDTFIHNKE
                     SVPILWLNDMLRSDFDIKSCESLSIILFNFQNSLIGVAATKIEGVTNELENIQSDRIT
                     GKGILGTAYKDNASILLLDISCLLEEFYRHKLKNIKLNLNDTDILPGA"
     gene            2812..5103
                     /locus_tag="PK683_00925"
     CDS             2812..5103
                     /locus_tag="PK683_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH07034.1"
                     /translation="MNYTDLIHYKKWKLNFKIASAFYFIGSVSLLIVGITSYMISRAA
                     MQEETFRRVTLVREAKASQIEDYFSIIRSQTQTYSENRSVIDAMREFKTGYNSIERES
                     VSEGRLASAKERVRKYYEKELMTRLKAGSADANLPSLLPRTNTEYILQDLYISSNSNE
                     VGKKNLLNAAKENYSYNAVHAKYHQTFMNYQQKFGFYDMFLVDVETGNILYTVFKELD
                     FSTSLLDGPYSSSNIGKVFKEVKNANQKGYVKLIDFEPYIPSYGAAGAFIGTPIFDKE
                     QMIGVLIFQLPLDKIMEIMNFHNSWKEAGMGKSGETFLIGQDLKMKSLSRFLIETPEE
                     YYKTLDNSDLPPETVQKIKTLKTNIGLQENKTKAGLDIAKGITGEAVYDDYRKVRVIA
                     SYKPLKIEDVKWGIISKIDEDEAFSEVVKLRNNLFIIAFFVFILIGLITFSLSRLIIK
                     PITAVVERVKDIGSGEGDLTKRLTIESNDELGELANWFNKFLVKIHSIVLEISSTSQI
                     ILASSNKLSETSLSLSASTEETSAQSELIASASTEMSQSVQIIASSIEEMSISISEVA
                     RRASDYAAISKEANQAAIETNIIVNKLGDDAKEIGKVIESIADIASQTHLLSLNAAIE
                     AASAGDAGKGFAVVASEVKELAKQSAISSQEIKEKIFSIQKNTESTITAINRIVTTIS
                     KVNDISSTIASAVEEQSITSKEISSNISQTSIGAREVTKNILGINKAARSGAQRAAQT
                     SELAEELQELTAGLEKIVKSFKI"
BASE COUNT         1759 a         1006 c         1105 g         1381 t
ORIGIN      
        1 ctgaacagca gacctgagga tctgctgccg ctttttcttg aattaaacga ggaaatatct
       61 gcagaagaaa ttctagtgat aaaattttcc gagctgcagg accttacctt ttcgatggca
      121 agtatggtaa gttcatttat tctgctcgta gaaaattcgc ataagacagc tgtttcgatt
      181 caggcagaac cggagctttc aaaaaaactg aaggaggcaa attttgaatc tatcgtatcg
      241 aagtttatgc cttcttagga gaaattaaat tggccggcat tgattataat gaattaaagg
      301 aaagctttgt caattcttct gctgctattc tgcagaggtc agaatcctgc attctgatcc
      361 ttgaatcttc cgttgataaa aatgctatag atgaactgtt tcggggcatt catactatta
      421 aaggcaattc cggaattttt gaattaaaaa atatccccag actgtctcac tcttttgaaa
      481 atgtactgaa tctgctcaga acatcccggc tgatgccgac acctgattta atcgacctga
      541 tgcttttagg aatagacaga cttcgggaga tgatcaaaga tctaaaagca tctgagtcgg
      601 tagacgcaga tgatcttatt gaaaaattca acgcttatct ttctcctgcg aaagacaaag
      661 aaaaagcatc aaaggattcg aagactgttt ctgaaatgca gatttcaaat gaagagacca
      721 aatggatgaa gcatataaag gtttcagata agataatcga atttgcaaaa tccaaaaaaa
      781 aatatctttc catagtcatt ttcagtctgg aagttcagca gttcaggaca gtttcaggac
      841 ttcttgaaaa aattcagatg atcgcaaaaa aagcaagagt tctcagaaag ggtttaatta
      901 caaaaaaact cacccctttg gaaaataaaa tcaatctgca ccttccctat ttcctgattc
      961 tgctatctga ttcaaaagta gaggaatttc tggaagaaat tggaattcag cctgagtttg
     1021 tcaggacagt cttcgtcccg gacaatgcag aatccgtgca tatcaaccct gacagacaga
     1081 atacggaaca aacacagaca gctaagcagg ttcatccgat aaaggaaatt tcagtaaaag
     1141 ataccagcat caacgtcaat ctggatctgc tgaatcatct catcaacctt acaggagaaa
     1201 tcgtgctcgc tcgcaatatt ttatcattaa agatactgca gagcggcaat tcagaactgc
     1261 agtccatttc aaaacggttc gaaaacctgc tttctgaact ggaatccgga attttaaaaa
     1321 caagacttca gagtatgaat gttttttttc agaaaatccc gcgacttgta agggatattt
     1381 cgaaggccac gggtaaacag attgatgttc atattgaagg cggcgacgta gagctggata
     1441 aatccctgat tgatgaagtc agtgatccgg tgactcattt aatccggaat gcaattgatc
     1501 atggaatcga aaattccgaa gaaagacaaa aaaaaggaaa aaacagtgtt ggtaacttaa
     1561 gagtatccgc aaggctgaga ggcggaaatg tggaactaat tatcggagat gacgggcggg
     1621 gactggatat tgtaaaaatc agagaaagaa ttatccagaa aaaccttgcc ccccctgaac
     1681 ttgttcatcg catacctaaa gaagaaatta tagaatactt atttatgcct ggattcagta
     1741 cggccggttc cgtaacggaa ctttcgggca gaggcgtagg cctagatgta gtcagatcca
     1801 atgttaaaag gatcggcggg acagtttcca ttgagtttga agaaaactca ggcacaagtt
     1861 tcatactttc cattccgcag accttgtcga tcatcaattg cctggttttc aaatccggaa
     1921 accgtaaatt cgcaattccg cagcagtatt tatgcgagct gataaaaatt aatccgataa
     1981 atataactaa aattgaaaat aaaagtcttt actatttaag gaaccagatg ctgccgcttg
     2041 taaatctgaa aaacgtgttt catattgaaa atgatgaatt aaagcaatcc gttgaaaaat
     2101 ttatcatagc cctcaagtcg gaaaaatact atttcgggat caatgtagat gaaattctgg
     2161 atacagagga aattgtagtg aagccgcttg gggagtattt tgaagaactc ccgttttttt
     2221 cgggcgtcac tgtactggga gacggagaca ttgcgcttat tctggatgtt tcaggacttg
     2281 caagactttc cggactgcag aactcacaga atctgatcga gtcctctgcc ttgaaagaaa
     2341 tcagcaaagc agaagaaatt tatcttttat tttctgtcgg agccgtcaaa gtcactgtgt
     2401 ctattgcttc caatccttac ataatcaaaa tttctgcaaa ggagattatc cattttctgg
     2461 gtctggatac ttttattcat aataaagaat cagttcctat actatggctg aatgatatgc
     2521 tgcgttcaga ctttgatata aaatcctgcg aatctctgag cattattctg ttcaatttcc
     2581 agaacagcct gattggagtt gctgctacaa aaattgaagg ggttacgaat gaattggaaa
     2641 acatacagtc agaccgaatc acaggaaagg gaattctagg cacagcatac aaagacaatg
     2701 caagcatact gctgctggat atttcctgtc tgctcgaaga attctacaga cacaagctga
     2761 aaaatataaa gctgaattta aatgacacag atattctgcc gggagcatag tatgaattat
     2821 accgatttaa tccattataa aaaatggaag ctgaatttta aaatagcctc tgcgttttac
     2881 tttataggct ctgtatccct gctcattgta gggataacaa gctatatgat ttcccgtgca
     2941 gccatgcagg aagaaacatt cagaagagtt acccttgtgc gtgaagcaaa agcctcacag
     3001 atagaagact atttcagcat cattcgcagc cagacgcaga catattcaga aaacagatcc
     3061 gtaatcgatg caatgcgtga gtttaaaaca ggttataatt caatagagag agaatcggtt
     3121 tcagaaggca gactggcttc agcaaaggag agagtccgaa agtattatga aaaggaactg
     3181 atgacccggc tgaaagccgg ttctgctgat gcgaatctcc cttcccttct gccccgcaca
     3241 aatacggaat atattctgca ggatctttac atcagttcaa actcaaacga agtcggcaaa
     3301 aaaaatcttc tgaatgcagc aaaggaaaat tattcataca atgctgttca tgctaaatac
     3361 caccagactt ttatgaacta ccagcagaaa ttcggttttt acgatatgtt ccttgtcgat
     3421 gtggaaacag gcaatattct gtataccgtc ttcaaggaac tggatttcag cacgtccctg
     3481 ctcgacggtc cttacagcag ttcaaatatc ggaaaagttt tcaaagaagt aaaaaatgca
     3541 aatcagaagg gatatgtgaa gctcatagat ttcgaacctt atattccttc ttacggcgcg
     3601 gcaggtgcat ttatcggaac accgattttt gataaggagc agatgatcgg agtcctcatt
     3661 ttccagcttc ccctggataa aattatggag ataatgaact ttcacaacag ctggaaagaa
     3721 gcaggaatgg gaaaatcagg agaaacattc ctgatcggac aggatttgaa aatgaaatct
     3781 ttatcccgat ttctcataga gacacccgaa gaatactata agacactgga taattctgat
     3841 ctgcctccgg aaactgttca gaaaattaaa accttaaaaa ctaatatcgg tctgcaggaa
     3901 aataaaacta aagcggggct ggatattgca aaaggaatta caggtgaagc ggtttatgat
     3961 gattaccgaa aagtaagggt aatcgcaagt tacaaaccac tgaaaataga ggatgtcaag
     4021 tggggaatca tcagcaagat tgatgaagac gaagcatttt cggaagtggt caagctgagg
     4081 aataatctct tcatcattgc cttttttgta ttcatcctga ttggactcat taccttttca
     4141 ctttcccgat tgatcataaa accgatcacg gcggttgtag agagggtgaa agatatcggg
     4201 agcggagaag gtgatctaac aaaacgactg acaatagaat caaatgatga attaggagaa
     4261 ctggcaaatt ggttcaataa atttttggta aaaattcatt ccatcgttct ggaaatttcc
     4321 agtacaagtc agatcattct cgcaagttca aataagcttt cagaaaccag tctgtccctg
     4381 tccgcctcca cggaagaaac ctctgcacag tcagaattga tcgcatctgc gagcactgag
     4441 atgagtcaga gtgtgcagat aatcgcttct tccatcgagg aaatgtctat ttcgatatca
     4501 gaggtggcca gaagagcaag tgattatgct gcaatctcaa aagaagcaaa ccaggcagcc
     4561 attgaaacca atataattgt aaataaactc ggagacgatg caaaagaaat cggaaaggtg
     4621 atcgagagca tagcggacat cgcatcgcag acacatctgc tttcactgaa tgcagccatt
     4681 gaagcagcaa gtgccgggga tgcggggaag ggttttgcag tagtcgcctc agaggtaaag
     4741 gaattggcca agcagtctgc catatcttcc caggaaatca aggagaaaat cttctcgatc
     4801 cagaagaata cagagagcac aattacggcc atcaacagaa ttgtaactac aatctcaaag
     4861 gtgaacgata tcagctccac gattgcctct gctgtcgaag agcagtccat cacctcgaaa
     4921 gaaatttcct ccaatatcag ccagacttcc atcggggcaa gagaagtgac aaagaacatt
     4981 ctgggaatca ataaggctgc cagatccggt gcacagagag cggctcagac atctgaactg
     5041 gctgaagaac tgcaggaact tacagcggga ctcgaaaaaa ttgtaaagag ttttaaaatt
     5101 taatatgcag actaaaaaac tttctaatgg tattctggaa gttcttacat ggtatgcagg
     5161 agatcaattg tacgggttat atcttgagga ttgcaaggaa gtcataaaga atatagaaat
     5221 tgtaactatt cctaactcca aaaaatacct t
//
LOCUS       DAOQGJ010000032         5223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8158_length_5223_cov_3.426471, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000032 DAOQGJ010000000
VERSION     DAOQGJ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8158_length_5223_cov_3.426471"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1247
                     /gene="serC"
                     /locus_tag="PK683_00930"
     CDS             165..1247
                     /gene="serC"
                     /locus_tag="PK683_00930"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNH07035.1"
                     /translation="MKRVYNFSAGPAMLPAEVLKQAQEEMMSWKDSGMSVMEMSHRGK
                     EYVSIAEKAEADLKELLAVPSNYKVLFLQGGASQQFAMIPMNLLGDAKTVDYINTGSW
                     SKKAISEAKKFANVNVAASSESAKFTTIPDPSEWKLSSDAAYVHYTPNETIEGVEFHW
                     VPDTKGVPLVADMSSTILSRPIDVSKFGMIYAGAQKNIGPAGLVIVIVREDLLERKKG
                     NIPTMLDYKTHVENGSMYNTPPTYSWYIAGLVFEWLKKNGGLSKMAEVNKAKAEKLYA
                     AIDGSGFYTNPVVPKHRSWMNVPFILKNADLDKKFLDEAKKVGLETLAGHRSVGGMRA
                     SIYNAMPTEGVDALISFMADFQKKNG"
     gene            1252..2427
                     /locus_tag="PK683_00935"
     CDS             1252..2427
                     /locus_tag="PK683_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNH07036.1"
                     /translation="MYKILTLDNISPKGLEKLPRSLYEVSSQMQDADAILLRSFKMHD
                     YTLPETLAAVGRAGAGVNNIPIDKLSQKGIPVFNTPGANANAVAELVLAGMLLASRNI
                     CQAWDFAKNLEGDDAEISKLVEAGKKKFVGTELPGRTLGVIGLGAIGVKVANNAMALG
                     MKVIGYDPVLTVERALELSSGIRKTTSMDEILTQCDFVTIHVPLNDETKNLLSEVRIR
                     KMKQNAVILNFSREAIVNEGAIVSAIESGRIGAYVCDFPSNKLKNQNRVIALPHLGAS
                     TEEAEENCAMMIADQIRDYIENGNIRNSVNFPDVEMARTDGNRITIANSNVPNMVAQI
                     STVLGQEGLNIIDMTNRSRKDLAYTILDVDKPVSDKSLQAIRSISGVLSVRKVGPAN"
     gene            complement(2944..3591)
                     /locus_tag="PK683_00940"
     CDS             complement(2944..3591)
                     /locus_tag="PK683_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH07037.1"
                     /translation="MGIVRVAAIGVSLDGFAAGPNQSLENPLGERGPELMSWVFPTKF
                     FQQMHGDGSANGTTGVDNDFAFRSFENVGAWILGRNMFGPIRGSWGDESWKGWWGDNP
                     PYHVPVFVLTHHPRKPLEMEGGTTFYFITEGIEAALEKAKAAAKGKDVRIGGGTQTIR
                     QYLQKGLIDEMHLAHSPVYLGQGENLLAGLDLPKMGFKITEQKQGEAALHIILKK"
     gene            complement(3755..4555)
                     /locus_tag="PK683_00945"
     CDS             complement(3755..4555)
                     /locus_tag="PK683_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07038.1"
                     /translation="MNHFYFLFCILFLIIQFPLNAHKSESSSKNYGIETGYKVLFLGS
                     SFSGRKEREFETEYAAFGKYITEKYKIFAGLSYISHSRKPSFANGFFEPYAFYHLNLN
                     YDTFQNKRFDVPIYAYTGFNYEIKEKYYIKYFGLKLGRFPGESFESWQGSPFSLLSPM
                     SLLGDKTNSCQNYERKIHRQYSALGVLELHGGWNFHIFEKLSLAVDLAVLFTSPNRKT
                     AADTYIDLNRLDGGDPVQLILLNKFMNGSKETGGSIGQSFSLGLHYIY"
     gene            complement(4664..>5223)
                     /locus_tag="PK683_00950"
     CDS             complement(4664..>5223)
                     /locus_tag="PK683_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07039.1"
                     /translation="FGDMFTLGLEQKTFGASAYLWCVGGGLEVSYWGEGFGLRNGHLG
                     TYRTGGTKAINSEEYTSSRKGRQKPIPGGLSFLVFNTMEHKPKGTKEYRNRKKRYIHS
                     NVGFIIPQNCHAPLTLEFSMGLFYGFRIGFNFSEFLDFHLGILGFDIMEDDRSRKTDS
                     EEQEILELKEKSHQEKEKMEKVRSE"
BASE COUNT         1556 a         1107 c         1091 g         1469 t
ORIGIN      
        1 caagactgct gaaaaaacca tttttttcaa agaatgatcc gacgttcaaa gatacggttt
       61 gataaaaatc agaaaatgaa gccctgaaag cacgaggagg catctcttaa aagaattgcc
      121 ccctgacctt taaaaaataa tttggaatca gggggaagat tctaatgaaa cgagtttata
      181 attttagtgc aggccctgcg atgcttcctg cagaagtgct gaaacaggct caggaagaaa
      241 tgatgagctg gaaagacagc ggaatgtccg taatggaaat gagccacaga ggaaaagagt
      301 atgtctccat cgctgaaaag gcagaggctg atctcaagga actgcttgca gtgccgtcca
      361 attataaagt tctttttctt cagggaggag cgtctcagca gtttgcaatg attcctatga
      421 atcttctcgg agatgctaaa actgttgatt atataaatac tggatcatgg tcgaagaagg
      481 ccatatctga ggccaaaaaa tttgcaaatg tgaatgttgc agcaagttct gaaagtgcaa
      541 agttcactac tattcccgat ccttccgaat ggaaactgag ttcagatgcg gcttatgtgc
      601 attatactcc gaacgagaca attgagggag tggagtttca ctgggttcct gatacaaagg
      661 gagttccgct tgttgcagat atgtcttcta caattctgtc ccgccctatt gatgtttcca
      721 agttcggaat gatctatgcc ggagcgcaga aaaacatcgg acctgcaggc cttgtgattg
      781 tcattgtgcg ggaagacctt ctggaaagaa aaaagggcaa tattcctaca atgcttgatt
      841 ataagacaca cgtagaaaac ggttcaatgt acaatacacc gcctacatat tcctggtaca
      901 ttgccggact ggtatttgaa tggctgaaaa aaaacggcgg tctctccaaa atggctgaag
      961 tcaacaaggc aaaagctgaa aagctttacg cagcgattga cggttccggt ttctacacaa
     1021 acccagttgt tcctaaacac cgctcctgga tgaatgttcc tttcattctc aagaatgccg
     1081 atctggacaa gaaattcctg gatgaggcaa aaaaagtcgg tctcgaaact cttgcaggtc
     1141 acagatctgt cggcggaatg agagccagca tttacaacgc tatgccgact gaaggcgtag
     1201 acgcactgat cagctttatg gcagattttc agaagaaaaa cgggtaactg aatgtacaag
     1261 attttaactc tcgataacat ttcgcctaaa ggtctggaaa aacttccccg ctccctctat
     1321 gaagtatcct cacagatgca ggatgcagat gcaattctgc tccgttcttt caagatgcat
     1381 gactacactc tgcctgaaac gttggccgca gtgggaaggg caggagcagg tgtaaacaat
     1441 atccctatag acaaactcag tcagaaagga attcccgtgt tcaacactcc cggagcaaac
     1501 gcaaatgctg tagctgagct tgtgcttgcc ggaatgctcc ttgcatccag aaacatctgt
     1561 caggcatggg actttgctaa aaacctggaa ggtgatgatg ctgagatttc caaacttgta
     1621 gaagcaggaa aaaagaaatt cgtcggaaca gaacttccag gaagaacact gggcgtcatc
     1681 ggtcttggtg ctatcggagt gaaggttgca aacaacgcaa tggctctggg aatgaaagtg
     1741 atcggatatg atccggttct cactgtagaa agagcacttg aactttcctc aggaatcaga
     1801 aagactacaa gtatggatga gattctcact cagtgtgatt tcgtaaccat ccatgtgcct
     1861 ctgaatgatg aaacaaaaaa tctgctgagc gaagtcagaa tcagaaagat gaagcagaat
     1921 gcagttattc tgaatttttc cagagaagca attgtaaatg aaggtgcgat tgtatcagca
     1981 attgagtccg gaagaatcgg tgcatatgta tgcgattttc cttccaataa actgaaaaac
     2041 cagaacaggg tcatcgctct tcctcatctg ggagcatcca ctgaagaagc agaagaaaac
     2101 tgtgcaatga tgattgctga tcagatccgt gattatattg aaaatggaaa catcagaaac
     2161 tctgtaaact ttcctgacgt ggaaatggca agaacagacg gtaacagaat cactattgca
     2221 aattccaatg ttccgaatat ggtcgctcag atttctacag tgctgggaca ggaaggtctg
     2281 aacatcattg atatgacaaa cagatccaga aaagatctgg cttatacaat tctggatgtg
     2341 gacaaacctg tatctgacaa gtcacttcag gcaatccgaa gcatcagcgg agttctctct
     2401 gtcagaaaag tcggtcctgc aaattaaaac aactgacgtt ggaaaaaaac caacgtcact
     2461 cttctttttt cagcctgctg aaatctgttt ctttattttt tcgagacttt cttccttgat
     2521 gacagccttc agtattttct gaagagtcgg gatgtcctgt attgccatta ctttcgtgaa
     2581 gatcgatttg gatttttgtc caaatttagt ttccagtgca atttgaatcg actctttgcc
     2641 ttgaatacaa aatcattgct cagaggaaga agatgcatat ctgcaatatt ttatattcta
     2701 ttataatgtt gaagatttta ttttaaaact cagtgtcctg cagagcagtt attcagccaa
     2761 gggtgtatga taacctttca caaaactggg tttccttgaa ttggaaacat aggaaaaaac
     2821 cagtaaatac tgaatatttt tattcggaca actgcttttc ctgattcgct tccccagaat
     2881 tcagttccta aaagaaggac ggttgtccgg ataaaaactg ggtgaagcga aaagactgca
     2941 cttttacttt ttgagtatga tatgaagagc tgcttcgccc tgtttctgct cagtaatctt
     3001 gaatcccatt ttaggcagat ccagaccggc taaaagattc tcaccctgac cgagatagac
     3061 gggggaatga gccaagtgca tttcatcgat gaggcctttc tgaagatact gtctgatcgt
     3121 ctgtgtacct ccgccaatgc ggacatcttt tcccttcgct gcggcttttg ctttttccag
     3181 tgcagcctca atgccctctg ttatgaaata gaatgttgtg ccgccttcca tttcgagagg
     3241 tttgcgcggg tggtgggtca gaacaaaaac aggtacatga tagggcggat tgtctcccca
     3301 ccagcctttc caagactcat ctccccagga gccgcgaatc gggccgaaca tattgcggcc
     3361 taaaatccat gcacctacat tttcaaacga acgaaatgca aaatcattgt ctacgcctgt
     3421 tgttccattt gcacttccgt ctccgtgcat ctgctgaaaa aattttgtag gaaaaaccca
     3481 gctcatcagc tcgggaccgc gttccccaag cgggttttcc aaactttgat tcggccctgc
     3541 ggcaaagccg tccagagaca caccaatagc tgctactctt actataccca tttgaactcc
     3601 ttaaagtatt acaattgtaa tatactgttc ttagaatcaa gttcatttag tgcatatctg
     3661 attttttagt atgggaagaa atttcactca tcaaatgtac tgtaaagagg actcacttgc
     3721 agaatctgcc ctgaagactt ctgaatatat aaaatcaata aatataatga agacctaagc
     3781 tgaaagactg tccgatagag cctcctgttt cctttgatcc gttcataaat ttattcaaca
     3841 gaatcagctg aacaggatct ccgccgtcca gtctgttcaa gtcaatatag gtatcagctg
     3901 ctgttttgcg attgggtgaa gtgaacagta ctgccagatc aactgccaaa ctcagctttt
     3961 cgaatatatg aaaattccag cctccgtgca gttcaagaac acctagtgct gagtattgtc
     4021 gatggatttt cctttcataa ttctgacagg aattggtttt atcgcccaat aacgacatag
     4081 gcgagagtaa actgaaagga gagccctgcc aggattcaaa ggactcaccg gggaacctgc
     4141 caagtttcag tccaaaatac ttaatgtaat atttttcctt tatttcataa ttaaaacctg
     4201 tataagcata gatagggaca tcaaatctct tattctggaa agtatcgtag tttaaattca
     4261 aatggtaaaa tgcataaggt tcaaagaaac cgttcgcaaa actgggtttc cttgaatgag
     4321 aaatatagga aagaccggca aatattttat acttctccgt aatatatttt ccgaatgctg
     4381 catactctgt ctcaaattca cgctcttttc ggccactgaa agaacttcct aggaataaaa
     4441 ctttatatcc tgtttctata ccgtaatttt ttgaagaaga ttccgattta tgcgcgttta
     4501 agggaaactg gataataaga aatagaatgc agaaaagaaa ataaaaatga ttcattttac
     4561 gtataagttc catatatcag ggacatattc tccatttcca atgaaatgtc accactgtaa
     4621 aacagatttt attctaacga ttttcactga cctgcggcct tactcattcc gatctcacct
     4681 tctccatctt ttccttttcc tgatggctct tttctttgag ttccagtatt tcctgttctt
     4741 ccgaatcagt ctttctgctt ctgtcatctt ccataatatc aaatccaaga atgccgagat
     4801 gaaagtcgag gaattcggag aaattgaatc ctattctgaa tccgtaaaaa agccccatcg
     4861 aaaattctaa agtcagagga gcgtggcagt tttgaggaat gataaaaccg acgttggaat
     4921 gaatataacg tttctttctg tttctgtatt ctttcgtacc tttgggcttg tgttccattg
     4981 tattgaatac tagaaaggaa agtcctcctg gaataggttt ctgtcttcct ttgcggctgc
     5041 tcgtgtattc ttctgagttg attgccttgg ttccgcctgt tctgtaagtg cccagatgac
     5101 cgtttctcag tccgaacccc tctccccaat aggaaacttc cagaccgcct ccgacacacc
     5161 aaagatatgc acttgcaccg aaagtcttct gttccaatcc cagggtaaac atatctccga
     5221 agt
//
LOCUS       DAOQGJ010000033         5216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8180_length_5216_cov_3.362333, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000033 DAOQGJ010000000
VERSION     DAOQGJ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_5216_cov_3.362333"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="PK683_00955"
     CDS             <1..1311
                     /locus_tag="PK683_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07040.1"
                     /translation="LEYISFFLSIALFPQFILVLFYDRRSGMIMKAVWGIVGAVTAVT
                     LFSKAVIYSYTAIPMQILILIEAVYLFYVMTVSMRKKSDGAVAAFSGMMILLFTIVLE
                     ILYQNEVSIFSAIPPIYVFPFGVFMFLFSQSFLLSQRFSKAFASVEQLSNYLLQTNDV
                     FSKFVPVEFLEHLNRENALDIRLGDQVQRDMTILFSDIRDFTSLSETMTPSENFNFIN
                     SYLKRMNPYIQDNSGFIDKYIGDAIMALFPRNAEDAVRAAVQMCREVKLYNEDRVLKN
                     YRPIQVGFGIHSGSLMLGIIGASSRMDSTVISDSVNLASRLEGLTKEYRVPLIVSESV
                     IRQIENTERFHYRLLDHVNVKGKLNSAVVYHVYDGQDPEEIILLDSIRKDFESGVKSF
                     HSGDYNNAYKFFRKTADLYPGDYPTELYLQRCSEKIAAAGASAE"
     gene            1324..1782
                     /locus_tag="PK683_00960"
     CDS             1324..1782
                     /locus_tag="PK683_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH07041.1"
                     /translation="MSLQTLKREQTLPVSMEKAWDFFSDPENLNEITPEDMTFRIMST
                     LPEKMYEGMLIHYKISPFLSIPLDWVTEITHIREKEFFIDEQRIGPYRIWHHEHHFRK
                     AENGILMTDLLNYDVGMGVLGSIAGAVFVHRKVRSIFDYRYEKLKNLFPG"
     gene            1992..3416
                     /locus_tag="PK683_00965"
     CDS             1992..3416
                     /locus_tag="PK683_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNH07042.1"
                     /translation="MESTLGKTSFFNEMFEMDSKVRHHYGSYNQIFASLSKKELSSRQ
                     FSAEKSFLFQGITFNVYKDNQNLEQIFPFDIIPRIISADEWTFLEKGLKQRVLALNLF
                     LQDIYNDRKIIKDKIIPDYLVQSSKGYLKECEGQTPPQNIWCHISGIDLIRHSDGKTY
                     ILEDNLRCPSGVSYVLENREVMKRVFPELFEKLKIRPVFEYPGKLLDMLHYIAPSENP
                     TVAVLTPGIYNSAYYEHSFLANQMGVHLVVGDDLVVQDDIVKVKTTKGLKKVDVIYRR
                     IDDTFLDPNVFRKDSLLGVKGLMQAYWKGNVALANAPGTGIADDKAVYAYVPDFIKYY
                     LGEDPILPNVPTYVCWNPKDMVYVLDHIDKLVVKAVNEAGGYGMLIGPHSTEKERNEF
                     REKVKANPRNYIAQPTMSLSQIPVWDSEGRLEGRHVDLRPYIIMGKETYIVPGGLTRV
                     ALKKGSLVVNSSQGGGSKDTWVLG"
     gene            3421..4380
                     /locus_tag="PK683_00970"
     CDS             3421..4380
                     /locus_tag="PK683_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNH07043.1"
                     /translation="MLTRVADSIFWMNRYIERMENNSRIIDVNLLISMELPTGTMEQW
                     GPLVHATGEYPLFEKLYGEPTKENVLKFLLAEKENSSSILNCLHFARENARIVRENIS
                     SEIWEHLNVSYLEVREMLQNDDVDILNIRDFLKKIKFIGQLFAGLIASTISRLEGWSF
                     SIMGRYLERADNASRFLDVKYFHLLPSPEDVGSSLDLIQWHSILKSLSAFEMYRKIYG
                     RISAADITEFIVLNHDFPRSILFCVLMLDAEMRKLTGSGINQFSNEAERKIGKLKADL
                     SYTSVSEIVKYGLHEYLDLLQRRLIEIGGEIDRSFLLSSGETV"
     gene            4856..>5216
                     /locus_tag="PK683_00975"
     CDS             4856..>5216
                     /locus_tag="PK683_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH07044.1"
                     /translation="MKKILILGAGISGNTAAMYAKAGGGKDIEVTVVSPGSRWNWIPS
                     NIWVGVGLMKPEQVTFELEPVYRKAGIIFKQAKALSIHPEGSETAANPFVRIEYTSPS
                     NQGVIEDVEYDFLINATG"
BASE COUNT         1522 a          987 c         1200 g         1507 t
ORIGIN      
        1 cttgagtata tttccttttt tctttcgata gccctgtttc cgcagttcat acttgtcctt
       61 ttttatgaca gaagaagcgg aatgattatg aaagctgtct ggggtattgt cggtgcggtc
      121 acagcagtca ctctttttag caaggcagtc atttacagtt atactgcaat accgatgcag
      181 atactcattc taattgaagc ggtgtatctt ttttacgtga tgactgtttc gatgcgcaag
      241 aaatccgacg gtgcagtcgc tgcattttcc ggtatgatga ttctgctttt tacaattgtt
      301 cttgaaatcc tttaccagaa tgaagtgagc attttttcag cgattccgcc gatttatgtg
      361 tttcccttcg gcgtgtttat gttcctgttt tctcagtcgt ttcttctttc gcagagattt
      421 tcaaaggcat ttgcctcggt ggagcagctg tccaattatc ttcttcagac aaatgatgta
      481 ttcagcaagt ttgttccagt ggaatttctt gaacatctga accgagaaaa cgctctcgat
      541 atcaggcttg gcgatcaggt acagagggat atgacgattc ttttttcgga catccgtgat
      601 tttacgagtc tctcggagac catgacccct tcggaaaatt ttaatttcat taattcttat
      661 ttaaaaagaa tgaatccgta tatacaggat aacagcggtt tcatcgacaa gtacatcgga
      721 gatgcgatta tggcgctttt tcccagaaat gcggaagacg ccgtccgtgc agctgtgcaa
      781 atgtgcagag aggtcaagct ctacaatgag gacagggtac tgaaaaacta cagaccgatt
      841 caggtaggtt tcggaatcca ttccgggtct ctgatgctcg gaatcatcgg tgcatcgagc
      901 aggatggaca gtaccgttat ttcagattca gtcaatcttg cttcgagact ggagggtctg
      961 acaaaggagt acagggttcc tctcattgtt tcggaaagtg taatccgtca gattgaaaac
     1021 actgaaagat ttcattacag acttcttgat catgtaaatg taaaaggaaa actgaacagt
     1081 gcagtagtat atcatgtgta tgacggtcag gacccggagg aaatcattct tctggattcg
     1141 atcaggaaag attttgaaag cggtgtgaaa tcctttcatt ccggagatta taataatgct
     1201 tataagtttt tcagaaaaac tgcagatctt tatcctggag attatccgac agaactgtat
     1261 ctgcagagat gttcggaaaa aattgccgca gccggtgctt cggcagaata aaggaggact
     1321 tctatgtcac tgcagactct gaaaagagaa cagacccttc cggtcagtat ggaaaaagcc
     1381 tgggattttt tttcagatcc cgaaaatctg aatgagataa cccctgagga catgactttc
     1441 aggataatga gcacccttcc tgaaaaaatg tatgaaggga tgctgattca ttataaaata
     1501 tctccttttc tttccattcc gctggattgg gtgactgaga tcactcatat acgggagaaa
     1561 gaatttttca tagacgaaca gagaataggg ccctacagaa tctggcatca tgaacatcat
     1621 ttcaggaagg cagaaaacgg aatactgatg acagatcttc tgaattatga tgtcggaatg
     1681 ggagtgctgg gaagtattgc aggagctgtg tttgtacaca gaaaagttag atctattttt
     1741 gattacaggt atgagaagct gaaaaacctc tttccgggct gaatcctgta tatagcaggt
     1801 gttttgcaga gtactctgtt ataaattcct tcttttcttt ccttgaacta tcgctaaact
     1861 ggcaggagtg gcatgatttt tgcttcttat ttcaatgttg aacgatttgc tataattttc
     1921 tctataacgg gtagggaaac aggaagtcct gcttaaaata caggcaaaac gcatatcggg
     1981 aagggggaac aatggaatca actttaggta aaacaagttt ttttaatgag atgtttgaaa
     2041 tggactctaa ggtccgtcat cactatggga gttacaatca gatttttgct tctctctcta
     2101 aaaaggagct ttcgagcagg cagttttcag cagaaaaatc ttttttattt cagggaatca
     2161 catttaatgt atataaagac aatcagaacc tggagcagat attccctttt gacattattc
     2221 ctagaatcat cagtgctgac gagtggactt ttcttgagaa aggtctcaag cagagagttc
     2281 ttgcactgaa tctgtttctt caggacatct acaatgacag gaaaatcatt aaagacaaaa
     2341 ttattcctga ctatcttgtt cagtcctcca aaggatatct gaaagaatgc gagggacaga
     2401 cccctcctca gaatatctgg tgtcatattt ccggaatcga tctgatccgt cacagtgacg
     2461 gcaagacata tatactggaa gacaacctca gatgtccttc cggtgtttcc tatgttcttg
     2521 aaaacaggga agtgatgaaa agagtattcc ctgaactttt cgaaaaactg aagatcagac
     2581 ccgtatttga ataccctgga aaacttctgg atatgcttca ttatattgca ccttcggaaa
     2641 atcctacagt tgcagtgctc actccaggaa tttataattc cgcatactat gagcattcct
     2701 tccttgcaaa tcagatggga gttcatcttg tagtaggtga cgatcttgta gttcaggatg
     2761 atattgtgaa agtgaagact acaaaaggtc tcaagaaagt cgacgtcatt tacagaagaa
     2821 ttgatgatac attcctggat ccgaatgtgt tcaggaagga ttctcttctg ggagtcaagg
     2881 gacttatgca ggcctactgg aagggcaatg ttgcacttgc gaacgctccc ggaacaggaa
     2941 ttgcggatga caaggctgta tatgcatatg ttcctgattt cattaaatat tatctcggag
     3001 aggatccgat tcttccgaat gtcccgactt atgtgtgctg gaatccaaag gatatggtat
     3061 atgttcttga tcatattgac aaacttgtcg tcaaggcagt gaatgaagca gggggatacg
     3121 gaatgctgat aggtcctcat tccacagaaa aagaaagaaa tgagttcaga gaaaaagtaa
     3181 aagcaaatcc aaggaattat attgcccagc ctacaatgtc gctttctcag attcctgtct
     3241 gggacagtga aggaagactg gaaggaagac atgtggatct ccgtccgtac atcattatgg
     3301 gaaaggaaac atatattgtt cccggaggac ttacaagagt cgctctgaaa aaaggttctc
     3361 tcgtggtgaa ttcatctcag ggcggaggaa gcaaagacac ctgggttctg ggttaatcat
     3421 atgcttaccc gtgtagccga ttcaatattc tggatgaaca gatatattga gcgtatggaa
     3481 aacaattcca ggatcattga tgtaaatctt ctgatctcaa tggagctccc tactggaact
     3541 atggaacagt ggggacctct tgtccatgca acaggagaat accctctttt tgaaaagctt
     3601 tacggcgaac cgactaagga aaatgtactg aagtttcttc tggccgaaaa ggaaaacagc
     3661 agttctattc tgaactgtct tcattttgca agagagaatg caaggattgt cagagagaat
     3721 atttcgagcg aaatctggga gcatctgaat gtaagttatc ttgaagtcag agagatgctt
     3781 cagaatgatg atgtggatat tctgaacatc agggattttc tgaaaaagat caaatttata
     3841 ggacagcttt ttgcagggct gattgcatcc actatttccc gtctggaggg gtggagtttc
     3901 agcataatgg gaaggtattt ggaaagagca gacaacgctt cccgttttct ggatgtgaaa
     3961 tattttcatc ttcttccgag tcctgaggat gtcggttctt cactggatct gattcaatgg
     4021 cattcgattc tcaaatctct cagtgcattt gagatgtaca gaaagatata cggcagaatc
     4081 agtgctgcag acatcacgga attcattgtc ctgaatcatg atttccccag gtcgatactg
     4141 ttctgtgttc tgatgctgga tgcagaaatg agaaagctga ccggttcagg aatcaatcag
     4201 ttctctaatg aggctgagag gaagatcggt aaactgaagg ctgacctgag ttatacttct
     4261 gtttccgaga ttgtaaaata cggcctccat gaatatctgg atcttcttca aagaagactt
     4321 atcgagatag ggggggagat cgacagatca tttcttcttt cttcaggcga aactgtctga
     4381 caggacaaaa ttcactttct tcagttcatt tttctgcgga aatttggact gaagttctgc
     4441 acagcttatc ctgatccgac tggacaaaaa agagtccatt tttccttttt cagtcatact
     4501 gatgcagata aaatcgtcac tgctcactga aaaacagatt atcaaagaat ttacagagaa
     4561 cagcttggac ttgctgtcct gcggcttcga ttctgaatac tgccgcttca gaaggagcag
     4621 tcatagaaaa tcttataaga cagtatgaaa tctgctgaat ctgcacagga aagaaaaaca
     4681 cagaaacatc agtgaccttt gaaaacaaca gtttcggaag aaagggttcg tgctatgcag
     4741 ctctttctcc cgccaatacc agcagctctg ttatattctg aaggctccgt tcaaaaaaag
     4801 gttgacctgt ttatatacct atactagtat atgtatataa cgcaatggag ggattatgaa
     4861 gaagatcttg atcctcggag caggcatttc cggaaatact gcggctatgt atgccaaggc
     4921 agggggagga aaggatattg aagtgacggt agtcagtccg ggaagcagat ggaactggat
     4981 tccctccaat atatgggtgg gggtaggtct gatgaaaccg gagcaggtga cttttgaact
     5041 ggaaccggtt taccgaaagg cagggataat tttcaagcag gctaaagcgt tgtcaattca
     5101 tccggaagga tctgaaacag ctgcaaatcc cttcgtcaga atcgaataca cgtctccttc
     5161 aaatcaggga gtgatcgagg atgtggagta tgattttctc atcaatgcga ccggac
//
LOCUS       DAOQGJ010000034         5196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8244_length_5196_cov_3.918109, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000034 DAOQGJ010000000
VERSION     DAOQGJ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8244_length_5196_cov_3.918109"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PK683_00980"
     CDS             <1..340
                     /locus_tag="PK683_00980"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH07045.1"
                     /translation="ERTGTVLGYSEKAKYKDRKIRFERGDRIIFYTDGILENLNSRNE
                     IFGLSGLLNSIQMTLFESEKRKVIEKIISDAKLFIGGGDFSDDATVLAVEHTGSPRHK
                     LAEITKNKYR"
     gene            387..4010
                     /locus_tag="PK683_00985"
     CDS             387..4010
                     /locus_tag="PK683_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH07046.1"
                     /translation="MKERFPSDDVSEIKNIPEYIRKIRLEKILLQLQNEQLQEHLKET
                     QSTQKRSKAILDTIPDLIFIVDREGKYRDYQTASPEKLFKPPEEFLGRSFSEILPEPL
                     ASDAMNAVIRTLETEKSQFIEYSIPVQNEKMYCEVRFFPYAKDQTLLIVRDVTAAKKA
                     EEELKNYRVHLEEIIEERTNQIYEEKEKSYALFETMTSGIVFQNAEGKIVSLNPAALD
                     ILGLSLEQMQGKTAKDIRWRAVHEDGTEYAYEDLPIRTALRTGKNVQDSILGIFHPVR
                     KEMRWLSVSAVPVFKTGEKTPYLVYSIIDDVTERIKANQQLKITNQAIEAATNGIIVT
                     DYQQMDNPIIYVNPAFERITGYSKEEVIGKNCRFLSRADRDQDSLDIIRDAVSRRKEC
                     KVLLRNYRKDGTLFWNELTVAPVKDEEGTVRFFVGVQQDVTERVQIEKDLEESRTKLK
                     AALESMTDAVFISDVNGNFIDFNEAFAIIHKFKNKSECRKTFSEYPKILELYHADGQP
                     ALLENWAVPRALRGEIVTNEEFILKRKDTGETWVGSYSFAPIYDNDGMIIGSVVVGRD
                     VSEEKRVQNKLKESEARLQLAVSAANIGLWEFDFNEKKILYISDIWKKQLGYSDAEVS
                     TSLEKLFQMLIHPEDLSSAYSRIMEIKDEKTKHLEIEFRVLHKNGYYVWILSRFFIIR
                     DINNKPVTIIGANLDITEHKQTEGILSYRVFERTKELAKINEELKLAKDLAESANSAK
                     SSFLANMSHELRTPLNAIIGYSQMFLEAGNLTMKQLQGIRTIFQSGQHLLTLISDILD
                     FSKIEAGKMELSPSDCFFHDFLNEIVSMIEIRSYRQGIGFTQQIDPEIPSVISADEKV
                     LKQILINLLGNAVKFTKKGSVTFKAECLSEKNSNTARIRFQVDDTGIGISEENLKIIF
                     DPFIQADRTNKNSNTEGTGLGLTISKKLLGLLNSELCFSSQVNSGSSFWFEAEFPVVR
                     KTKGEIHDKKYRKLSGYSGERKTILIVDDNTANRNLLVDFFQPLGFFINEARNGEEGL
                     NSFLQEKPDFILMDMIMPGMDGKECTGKIRECEQISRLEKTPIIALSANISDVSREEA
                     IQCGCNDFIKKPIDFRQIAVMLEKYLNLEWTEDSDNEISWMNIQEIEIEKLVRELPAL
                     HRENLCKSAEIGDTKEIINELEIIDELDSRFLPLVSRLKSLAMDFNNDIILEMLQKGD
                     SGI"
     gene            4007..5116
                     /locus_tag="PK683_00990"
     CDS             4007..5116
                     /locus_tag="PK683_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNH07047.1"
                     /translation="MTDRIFRILIVDDNKNNIETLFALLDEKGYKIITSRSGENALKI
                     LEKTLPDLILLDILMPPGMDGIETCRKIKENPATEDVPVIFMTALTDTVDKVKGFEVG
                     AVDYITKPFQTEEILARIKTHLSIQKLKTDLKASLEKEKTLNMQKSKFMSMILHDLRT
                     PLAVIQSTSDLLFLKGDAIDAEKREKYHGSIDRSISKMTEMMDSFLIMTKAESGKFDF
                     HPEFCDMEKLCIETADSFREIGRDTHSISLNFLGNDFIINADPKLIQNTIANLLSNAI
                     KYSPRGGNIDFEVKADSSGIQFKVKDSGIGIPNEETGNLFQPYFRAANAAKIKGTGLG
                     LSIVRQFVELHNGKIRLESEVNKGTTVTVEIPRQV"
BASE COUNT         1770 a          935 c         1186 g         1305 t
ORIGIN      
        1 ggagagaacg ggaaccgtat tggggtattc tgaaaaggca aagtacaagg acagaaaaat
       61 cagatttgaa agaggagaca ggatcatttt ttatacagac gggattctgg aaaatttgaa
      121 cagtcgaaat gaaatttttg gtctgtccgg tcttttgaac agcattcaga tgactctttt
      181 tgaatcagaa aaaagaaaag ttattgaaaa aattatttca gatgctaaat tatttattgg
      241 gggaggggat ttttcggatg atgcaacagt tctggcggta gagcatacag gaagcccccg
      301 tcataaactg gctgaaatta caaaaaataa atacagatag ggagtctagt cttatgatgt
      361 ccgttcagca gaaagcagaa gagttaatga aagaaagatt tcctagtgat gatgtcagtg
      421 aaataaaaaa tatcccagaa tatatcagga aaattcgcct cgaaaaaata cttcttcaac
      481 tccaaaatga acaactccag gaacatctga aagaaaccca aagcactcaa aagcggagca
      541 aggccattct ggatacaata cctgatctga tttttattgt ggacagagag ggcaaataca
      601 gagattacca gacggcatca cctgaaaaac tgtttaaacc ccctgaagag tttttaggca
      661 gaagcttcag tgaaatactt cctgaacctc tggcttccga tgcaatgaat gcagtcataa
      721 gaacgcttga aacagaaaaa tctcagttta ttgaatattc tattcccgtg cagaatgaaa
      781 aaatgtactg tgaagtaaga ttctttcctt atgcaaaaga tcagactctt ctcattgtca
      841 gagatgtgac agcggcaaaa aaagcggaag aggaattgaa aaactataga gtgcatctgg
      901 aagagattat tgaagaaaga acaaatcaga tttatgaaga gaaggaaaag tcctacgcac
      961 tgtttgaaac gatgacaagc ggaattgtct ttcagaatgc agaagggaag atcgtttcct
     1021 tgaatcctgc tgcactggat atccttggac tgagcctgga acagatgcag ggaaaaacag
     1081 caaaagacat tcgatggagg gcagttcatg aagatggaac tgagtatgct tatgaagatc
     1141 tgcccatccg aactgccctt cgtacaggaa aaaatgttca ggattccata ctcggaattt
     1201 ttcatcctgt ccgaaaggaa atgcgatggc tgagtgtttc cgctgtgcct gtttttaaaa
     1261 ctggggagaa aactccctat ctggtatatt caattattga tgatgtaaca gagaggataa
     1321 aggcaaatca gcagctgaag atcacaaatc aggcaattga agcggccacg aatggaataa
     1381 ttgtcactga ttatcagcaa atggataatc caataatcta tgtaaatcct gcgtttgaaa
     1441 gaatcactgg atattcaaaa gaggaagtca ttggaaaaaa ctgcagattc ctgagcaggg
     1501 ccgacagaga tcaggattcg ctggacatca tcagagacgc tgtgtccagg agaaaagagt
     1561 gcaaggttct gctgagaaat tacagaaagg atggaactct gttctggaat gaactgacag
     1621 ttgctcctgt gaaagacgag gaaggaaccg taagattttt tgtaggggtt cagcaggatg
     1681 tgactgagag ggtccagatc gaaaaagatc tggaagaaag caggacaaag ctgaaggccg
     1741 cccttgaaag catgacggat gcagttttca tatctgacgt caatggaaat tttatagatt
     1801 ttaatgaagc ctttgcaatc atccataagt tcaagaacaa atccgaatgc aggaagacat
     1861 tttcggaata cccgaaaatt cttgaattgt accatgccga cggacagccc gcccttttgg
     1921 aaaattgggc agttcctaga gctcttcgag gtgaaatagt cacaaatgaa gaattcattc
     1981 tcaaaagaaa ggacacaggt gaaacctggg ttggcagtta cagcttcgct cccatttatg
     2041 acaatgatgg aatgatcata ggttcagtcg tggtgggaag ggacgtgtct gaggaaaaga
     2101 gagttcagaa taagctgaag gaaagtgagg caagactgca gctggcagtc tctgctgcaa
     2161 atatcggact ttgggaattc gattttaatg agaaaaaaat actttatatt tcggatatct
     2221 ggaaaaagca gctaggctat tcggatgcag aagtgagcac ctctcttgaa aaactgtttc
     2281 agatgctgat tcatcctgag gatttgagtt ctgcctattc caggataatg gaaataaagg
     2341 atgaaaagac aaaacatctg gagatagaat tcagagtcct tcataagaat ggatactatg
     2401 tgtggatatt atctagattt tttataatca gagacattaa taacaaacct gttacaatca
     2461 taggagcaaa tctcgacatt accgagcata agcagacaga gggaattctg tcttacagag
     2521 tctttgagag gactaaggaa ctggccaaaa taaatgaaga gctgaaactt gcaaaggacc
     2581 ttgccgagtc tgcaaacagt gcaaagagca gttttcttgc aaatatgagt catgaactca
     2641 gaacgcctct gaatgcaatc atagggtatt ctcagatgtt tctcgaagca ggcaatctga
     2701 cgatgaaaca gcttcagggg attcggacta tttttcagag cggtcagcat ctgcttacac
     2761 tgatcagcga catccttgat ttttcaaaga tagaagcagg aaaaatggaa ctcagtccct
     2821 ctgactgttt ttttcatgat ttcctgaatg aaattgtatc tatgattgaa atccgttcct
     2881 acagacaggg aatagggttt actcagcaga ttgatccgga aatcccaagt gtaatatctg
     2941 cagatgaaaa ggtgctgaag cagattctga tcaatcttct gggaaatgct gtcaagttta
     3001 caaagaaagg aagtgtgact ttcaaagcag agtgcctctc tgaaaaaaat tcaaatacag
     3061 ccagaatcag atttcaggta gatgatacag gaataggaat ttcggaagaa aatctgaaga
     3121 tcatatttga tccctttatt caggcagaca gaacaaacaa gaattccaat acggaaggaa
     3181 ctggacttgg tcttacaatc tcaaagaaac tgcttggact tcttaacagc gaactctgtt
     3241 tcagcagtca ggtcaattca ggaagttctt tctggtttga agcagaattc cctgtcgttc
     3301 ggaaaactaa aggagaaatt catgataaaa aatatcgcaa actttcaggg tattccggcg
     3361 agaggaagac aatacttatc gtagacgaca ataccgcaaa cagaaatctc ctggtagatt
     3421 ttttccagcc actgggtttc tttataaacg aagcaaggaa cggagaggag ggactgaatt
     3481 ccttcctgca ggaaaagccc gactttatac tcatggatat gataatgcct ggaatggacg
     3541 ggaaggaatg cacaggaaaa atcagagaat gtgaacaaat atccagactg gaaaagaccc
     3601 ccatcattgc tctttccgcc aatatttctg atgtatcccg agaagaggca attcaatgcg
     3661 gatgtaatga ttttataaag aaaccgatcg acttcagaca gattgctgta atgctggaaa
     3721 agtatctgaa cctggaatgg acagaagact cagacaatga gatttcctgg atgaatattc
     3781 aggaaataga aatagaaaaa ctggtacggg agcttccagc tctgcacagg gaaaacctct
     3841 gcaaatctgc tgaaatcgga gatacgaaag aaatcataaa cgaacttgaa atcatagatg
     3901 agctggacag cagatttctt cctcttgttt caagactcaa atccctggcg atggatttca
     3961 acaatgacat tatactggaa atgcttcaaa aaggagattc aggaatatga cagaccgaat
     4021 attcagaata ctcatcgttg atgacaataa aaacaacata gagactctat tcgctcttct
     4081 tgatgaaaag ggatataaaa tcattacatc ccgaagcggt gaaaatgcac tgaaaattct
     4141 ggaaaagact cttcctgacc ttattcttct ggacatactg atgcctcctg gaatggacgg
     4201 aattgagacc tgcagaaaga taaaggagaa tcctgcaact gaggatgtac ctgtcatctt
     4261 tatgaccgca ctgacggata ctgtcgacaa agtgaaaggg tttgaggtcg gagcagtgga
     4321 ttatattaca aaaccttttc agacagaaga aattcttgcg agaataaaga cgcatctttc
     4381 catccagaaa ctgaagacag acctgaaggc atccctggaa aaggaaaaaa ctctgaatat
     4441 gcagaaatcc aagtttatgt cgatgattct tcatgactta agaactcctc tggcagtcat
     4501 tcagtccaca tcggatctgc tctttctgaa aggggatgca attgatgcag aaaaaagaga
     4561 aaaatatcac ggatcaatag acagatctat ttccaaaatg actgaaatga tggacagctt
     4621 tctgattatg acaaaggctg agtcagggaa atttgatttt catcctgaat tctgcgatat
     4681 ggaaaaactc tgcattgaga ctgcagacag ctttcgcgag atcggaagag acactcacag
     4741 tatttcattg aatttccttg gtaatgattt tatcattaat gcagatccca agctgattca
     4801 gaacacaatt gcaaatctgc tttcgaatgc aataaagtat tctccaaggg gcgggaatat
     4861 tgatttcgaa gtaaaggcgg acagttccgg catacagttt aaggtaaaag attccggcat
     4921 aggaattccg aatgaagaaa ctggaaatct ttttcagccg tatttccgtg cagccaacgc
     4981 ggcaaagatt aaggggactg ggctgggact ttctattgtc cgtcagtttg tggaactgca
     5041 taatgggaaa atcagactgg aaagtgaagt gaacaagggc acaactgtga ctgtggaaat
     5101 cccgagacag gtctaaactc attgggagtt tccgcactat gtgagtcctt aagccgctga
     5161 cagggcctgg cacattactc ggtgatcgcc ttcaaa
//
LOCUS       DAOQGJ010000035         5167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8311_length_5167_cov_3.284429, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000035 DAOQGJ010000000
VERSION     DAOQGJ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8311_length_5167_cov_3.284429"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PK683_00995"
     CDS             <1..895
                     /locus_tag="PK683_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HNH07048.1"
                     /translation="NRLTGGAPADRQDWLENWILENSEPTQYFRKLGVEFPQRVNPKK
                     YAPKTKKEAPQKANSNEQIIVLAARKICSLVKKNGYKSILAGIGAAHISAWLASLLLK
                     KEGIQIPVLAELGFYDVTPYKGDVFLFSQLHADNSASLTDIQGILGTAVPDRCLGVLG
                     AAEIDLLGNLNSTLSADGKFIVGSGGANDIASTADCLAVARIGKGRAVSKVSYVTSPG
                     KRVISLVTQFGVFDKNGAEEFSLSSFLPVPEKPDLNASECIEKFSDWKIGTEEFLQEE
                     GVLELELAILRELDPERIYIG"
     gene            complement(900..981)
                     /locus_tag="PK683_01000"
     tRNA            complement(900..981)
                     /locus_tag="PK683_01000"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Tyr,seq:gta)
     gene            complement(1036..1108)
                     /locus_tag="PK683_01005"
     tRNA            complement(1036..1108)
                     /locus_tag="PK683_01005"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1073..1075),aa:Thr,seq:tgt)
     gene            complement(1495..1737)
                     /locus_tag="PK683_01010"
     CDS             complement(1495..1737)
                     /locus_tag="PK683_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07049.1"
                     /translation="MHININTEDRNSIFLKRLLGSDSSFFTQFGNNSGCLSFLTANLF
                     PSIAKKREFMSGKFALAAGGSRGIGKSAEIKSAEQG"
     gene            1819..2274
                     /locus_tag="PK683_01015"
     CDS             1819..2274
                     /locus_tag="PK683_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07050.1"
                     /translation="MKKHFNIILSIAAVLTFGLNADDRHKGHGKNPCKDDAAKLCSGV
                     QKGGGKIWRCLNEKKSQVSKDCKAQLDKASEMMKKHGEKIKAACGSDAKKLCSKAPRE
                     KGGVFKCLKQNESKLSPSCKDAMSPPWKKNKGKDDDDDDSNDADHPDED"
     gene            complement(2429..4801)
                     /locus_tag="PK683_01020"
     CDS             complement(2429..4801)
                     /locus_tag="PK683_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07051.1"
                     /translation="MKIKYKFFLAFLLLNIALSALFGISMYFVAYQLFFKQFRDSKTA
                     LVKNIAFSIDGDQHRNLRDESASGKPEYRKLVSYLESVFSEETFIRFLYTFNYEERTD
                     SFYYVGTGFRLSRDTVWIEADHFAFSIYAKEDGSISVSYDDNVYESGSFITDDGIKKY
                     KFNIKKKDNKEYIFLEDAEFAEILNSSPLSVRFFAKSAVNEKENSIKGDFSLHQDKMS
                     YSIWFTKKGDLDLTPGQPLINSNLNSIKEQMKTGKVFTSESFLRDAYGSFLVIRAPIK
                     DRTGKTVGFTEANISEKQVIHFQESVRGVAFFVFIIVFIVSLAVSMMLSVYFTKPIQI
                     LTESTVQLAKGDLNYRISMRSKDEFGRLADSFNSMASSIESSANMILQTNIAYRRFVP
                     EEFLHFLNKDNIIDVGLGEYIQKKMTVLFCDIRDFTSLSEKMTPKENFLFLNAFLERM
                     EPIIKKNYGFIDKYIGDAIMALFSESPDDALDAAVEMIQTLGRFNEERSEKGLPAIRI
                     GIGLNFGELMLGTIGGESRMDSTVIGDTVNLTSRLEAVTKDFHVPLIVSETLVKNLKS
                     SERYCLREIGSVKVKGKEENVTVYECFSHEPADTIQRKKQSLDFFNFGIQHYKQKAYS
                     EAAKMFSKCQTLCPEDPVPVIYLYRCRTLMQNRAEQNFFDLKEEVWYPKKILVADDNK
                     AVLDLLTGFLRKNNFQTIKAESVSEAVDKFNLYRPEYLITDFYFSNGINGDRLISHIR
                     SRSNETGSRVKIAVVSAEDPGALKEKLKDFNADLVLSKPFQWEDILGFLK"
     gene            complement(4972..>5167)
                     /locus_tag="PK683_01025"
     CDS             complement(4972..>5167)
                     /locus_tag="PK683_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07052.1"
                     /translation="SKDRRRHLHRQVDNYNCDMTHVTDRKTSPETLIFTKTTRAYDRK
                     MKIADTNQKLLSGLRKKLRK"
BASE COUNT         1494 a         1124 c         1022 g         1527 t
ORIGIN      
        1 caatcggctg accggaggag ctcctgcaga ccggcaggac tggctcgaaa actggattct
       61 ggaaaattcg gaacccacgc agtattttcg gaaactggga gtggagttcc cacagagagt
      121 caaccccaaa aaatacgccc ctaaaacgaa aaaggaagca ccgcagaagg caaattcgaa
      181 tgaacagatc attgtgcttg ctgcacgtaa aatctgctcg ttggtgaaaa aaaacgggta
      241 taagtcgata ctggctggaa taggtgccgc acatatctct gcctggctgg cttcccttct
      301 tctaaaaaaa gagggaattc aaatccctgt tctggcagag ctcggcttct atgatgtcac
      361 tccttataaa ggagatgttt ttttattcag ccagcttcac gcagacaatt ccgcttcttt
      421 gacagatatt cagggaattt tggggactgc agttccggac agatgccttg gggttttagg
      481 agcggcagaa atcgatcttc tcggcaatct aaattcaaca ctcagtgcag acgggaagtt
      541 catcgtcggt tcaggcggtg caaatgatat cgcttccaca gcagactgtc tggccgttgc
      601 gagaataggc aagggcagag ctgtctccaa agttagttat gtgacctcac cggggaaaag
      661 agtgatttct cttgtcactc agttcggagt ctttgataaa aacggtgcag aagaattttc
      721 actcagcagc tttcttcctg ttcctgaaaa accggatctg aatgcatctg aatgtatcga
      781 aaaattctct gattggaaaa ttggaactga ggagttccta caggaagaag gggttctgga
      841 attggagtta gctattctta gagagctgga cccggagagg atttatatcg gataaaaagt
      901 gggcagggga ggattcgaac ctccgaaggc gtagccggca gatttacagt ctgatctcgt
      961 tgaccacttg agtacctacc cgtttttgtg aaaacaggaa cttttttgaa cgctgcttac
     1021 tccgaaatta aagaaagctg cctaagggaa tcgaaccccc aacctgctga ttacaagtca
     1081 gctgctctac caattgagct aaggcagcgt tcattgccaa tgttttttat agacagtaca
     1141 agtcaagcac aaaatagata atttttgcaa gactgttttc tacatttttg atcgtgcatt
     1201 cgacatcatt caatcttcga gaagattttt cctatatcat cagcttccct gcccttccaa
     1261 gtgcggtcat ggaagaaaaa cctgctttca tctgacgatt gttccgtatt acagcgtacc
     1321 ataagcccgg ggcaggaaca cctcctgaac ccagttcttc tgccagatac cctatgaata
     1381 cttatacggc acctgcatgg aggcgtatat agaatatcat aaaacacaat accttgcggt
     1441 gcggaggaaa tatgaataac tcttcctttt tttgtgatat tctataatta gattctaacc
     1501 ctgctctgca gactttatct cagcactttt tcctatgccc ctgcttccgc ctgcagccaa
     1561 tgcaaattta ccgctcataa attctctttt ttttgcaatt gacgggaata gatttgcagt
     1621 caggaaactt aaacatcctg aattatttcc gaattgagta aagaatgaac tgtctgaacc
     1681 caaaagacgt ttcagaaaaa tggaatttct gtcctctgta tttatgttga tatgcaaatt
     1741 cggatctgac tttttttgct ttgccgtttt caaaagcagt ctaaggtagg taatcggaat
     1801 gattccaaag gaaccactat gaaaaaacac tttaatataa ttctctcaat tgctgctgta
     1861 ctgacattcg ggctgaatgc cgatgacagg cacaaaggac acgggaaaaa tccatgcaag
     1921 gatgatgcag caaaactctg ttcaggagtt cagaaaggcg gcggaaagat ttggagatgt
     1981 ctgaatgaga aaaaaagtca ggtatccaag gactgtaaag ctcagctgga taaggcatct
     2041 gaaatgatga aaaaacacgg agaaaagata aaggcagcct gcggaagtga tgcaaaaaaa
     2101 ctctgctcaa aagcacctag agaaaaaggc ggtgtattca aatgcctgaa gcagaatgaa
     2161 tcaaaactgt ctccttcctg caaggatgca atgtctcctc catggaagaa aaacaaagga
     2221 aaagatgatg acgatgatga cagcaatgat gctgatcatc cggacgaaga ttaattccaa
     2281 cttcagaggc tgcctaaaac agcctctgga aaattctcat tccgatcatc agaaaatttt
     2341 ttcctttcag actccgctgc attcagggca gaccgaatta aagcaattgt cagtcagtag
     2401 accattttat aatagaattt actgtaagtc actttaaaaa accgagaata tcttcccatt
     2461 gaaaaggttt gctcagaaca agatctgcat tgaaatcttt cagcttttct ttcagtgcac
     2521 cgggatcttc tgcactgaca actgcaattt ttactctgct gcctgtttca tttgaccttg
     2581 agcgaatatg actgatgagc ctgtccccgt taattccgtt tgaaaaataa aagtctgtga
     2641 tgagatactc aggtctgtac aggttgaatt tatccactgc ttcagatact gattctgcct
     2701 tgatcgtctg aaaattattc tttctcagaa accctgtcag aagatcaagc accgccttgt
     2761 tgtcatcagc taccagtatc tttttgggat accacacttc ttctttcagg tcaaaaaagt
     2821 tctgttctgc ccggttctgc ataagagttc tgcatctgta aagataaatg acaggcaccg
     2881 gatcttcagg acacaatgtc tggcatttgg aaaacatttt tgctgcctcg gaatacgctt
     2941 tctgcttata atgctgaatt ccaaaattga aaaaatccag tgactgtttt tttctctgaa
     3001 tggtatccgc cggctcatgg ctgaaacatt cataaaccgt tacattctct tccttgcctt
     3061 tgactttgac gcttccaatt tctctgagac agtatctctc tgacgatttt aaatttttca
     3121 ccagagtttc cgaaacgatc agcggaacat ggaagtcttt agttacagcc tcaagtctgg
     3181 aagtcagatt tacagtgtca ccgatcacag tactgtccat cctgctttca ccgccgattg
     3241 taccgagcat aagttccccg aaattcagcc cgattcctat cctgattgcc ggcagaccct
     3301 tttcagatct ttcttcattg aatcttccca gagtctgaat catttcaaca gctgcatcca
     3361 gcgcatcgtc aggactctcg ctgaaaagag ccataattgc atctccgata tatttatcaa
     3421 tgaaaccgta atttttttta atgataggtt ccattctttc caaaaatgca ttcagaaaca
     3481 gaaagttttc ctttggcgtc attttctccg aaagagaagt aaaatcccga atgtcgcaga
     3541 acagcacggt catttttttc tgaatgtatt ctcccagtcc gacatcaata atattgtcct
     3601 tgttcaggaa atgcaggaat tcttcaggaa caaatcgcct gtaggcaata tttgtctgca
     3661 gtatcatatt ggcggaggat tcaatggagc ttgccatact gttgaatgaa tctgccagtc
     3721 tgccgaattc atccttgctc cgcatactga tccggtagtt cagatctcct ttggccagct
     3781 gcacagtgga ttctgtcagg atctgaatgg gttttgtaaa gtatacactc aacatcattg
     3841 aaactgcaag gctgactatg aatacaataa taaaaacgaa aaatgccaca cccctcacag
     3901 attcctgaaa atgaattacc tgcttctcag aaatgttcgc ttcagtgaat ccgactgtct
     3961 ttcctgtccg atctttaata ggggcccgaa ttactagaaa gcttccgtaa gcatctctca
     4021 gaaaactctc acttgtaaaa acctttcctg tcttcatctg ctctttgata gaattcagat
     4081 tggaattgat aagaggctgt ccaggagtca gatcgaggtc tccctttttt gtaaaccaga
     4141 ttgaatacga cattttatcc tgatgaagag aaaaatctcc cttgattgag ttttcttttt
     4201 cattcactgc tgactttgca aaaaatctta cagagagagg agaggaattc agaatctctg
     4261 caaattctgc atcttccaga aaaatatatt ctttattatc cttcttttta atattaaact
     4321 tatatttttt aatcccatca tctgtaataa agctcccgct ctcgtacaca ttgtcatcat
     4381 aacttacaga aatgcttccg tcttctttgg cataaatcga aaatgcaaaa tgatccgctt
     4441 ctatccagac agtgtcccgg gatagcctga accctgttcc gacataatag aaagaatctg
     4501 tcctttcttc ataattgaag gtatagagaa acctgatgaa ggtttcctct gagaaaactg
     4561 attccagata ggaaaccagt ttcctgtatt ccggctttcc ggaagcagat tcatctctca
     4621 agtttctgtg ctgatcgccg tcaatggaga atgcaatatt ttttaccaag gcagtcttgc
     4681 tgtccctaaa ctgcttaaaa aaaagctggt atgcgacaaa atacatcgaa attccgaaaa
     4741 gtgccgagag cgcaatattc aagagcagaa aagcaagaaa gaacttatac ttaatcttca
     4801 ttttcctcaa aactcttttt ttcctttttc agtgtatttc tcaaatcaga ttcttaaacg
     4861 ataagtttaa ggaatgccag tttcagtcaa taaaatcggc tatactgatt tttaattttg
     4921 agacagtgct gcagagattc ttccagtttt cattatgaac tgcagagttc atcacttcct
     4981 cagttttttt ctgagcccgc tcaggagctt ctgatttgta tctgcaattt tcatcttcct
     5041 gtcataggct cgggttgtct ttgtaaatat cagtgtttcc ggagatgttt ttctgtccgt
     5101 aacatgagtc atatcgcagt tataattatc gacctgcctg tgcagatgcc ttcttctgtc
     5161 tttcgat
//
LOCUS       DAOQGJ010000036         5123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8431_length_5123_cov_3.423441, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000036 DAOQGJ010000000
VERSION     DAOQGJ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8431_length_5123_cov_3.423441"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..587)
                     /locus_tag="PK683_01030"
     CDS             complement(171..587)
                     /locus_tag="PK683_01030"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNH07053.1"
                     /translation="MKNILVLCTGNSCRSQMAEGWLRHFAGEKAKVWSAGIETHGVNP
                     KAVLYMKEAGIDISGHTSNNVSEYMNINFDFIITVCDHAKENCPYIPSKAERFHHNFF
                     DPSKVKGTEEEIAVAFRRTRDEIRDYCSKFIEEKVK"
     gene            654..1982
                     /locus_tag="PK683_01035"
     CDS             654..1982
                     /locus_tag="PK683_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07054.1"
                     /translation="MKNMLQTVCTVYAEEKGLPQRTPIHLYEKMNTEEIIKSLEEELG
                     IILIRTQLENVLDDDFCFANEVHICRGMIISLGICKLDISALPKSLGQAIDLKYLTID
                     NCVNLIHFESLANLKDLDGLRLCNLSIVSLPSEISNLPNLKKIYLKSNSFLESFNSLA
                     YLYNLEELYLADLEIKKLPQEIRCNKKLKKIALNFSENLFDYNVLKFIKNTLEYFEIT
                     FEKYSRVPKIVRNFKNLKVFFMKTTYGFKDISNLKKLRHLKEITLAGLKTDALPKSIN
                     TLKNLEVLRIYEADPINADKLKGLKNLKHLIGTKPPKYAYTFHKLETLEAASYDLTDV
                     SMIKNLKRLSELSLRVHQAAELSEELFTCSNLISLAIEANLEYIPESVSKLEKLEELT
                     LRNCMNLKDIQAINKLKNLQLLRIFELTNRDIERQIMPRENLHLDIRYID"
     gene            complement(2168..2662)
                     /locus_tag="PK683_01040"
     CDS             complement(2168..2662)
                     /locus_tag="PK683_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HNH07055.1"
                     /translation="MEKNKKSLEFSVFPLWKFLSENYGMEEAFRMFGPYRGASVSPSI
                     IDKNTVEVSMPLTVSNTNYVGTHFGGSLYSMCDPFYMFLLIRNLGDDYMVWDKSASIE
                     FLKPGKGIVKVLFHISDAELEEIKNITAKEKKTTRFYETEIKDESGVTVARVKKELYI
                     RKLK"
     gene            complement(2664..3239)
                     /locus_tag="PK683_01045"
     CDS             complement(2664..3239)
                     /locus_tag="PK683_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenesis-related family 1 protein"
                     /protein_id="HNH07056.1"
                     /translation="MKYTIFSLMFLIQCSIISPEKEKNNNASIAAVAVLASASSSSAT
                     EYSAVSGITSEHNKVRTQENAGLPSLSWDATVGAYSKSKVEYLANNGCQLSHTAGPTN
                     PGYGENLFWGSGSSWTVTDAVTSWYSEKSNYTYSSNSCASGKVCGHYTQVVWKNSTKL
                     GCYLANCPSNAGMIIGCNYDPPGNYNGQKPY"
     gene            complement(3551..4318)
                     /locus_tag="PK683_01050"
     CDS             complement(3551..4318)
                     /locus_tag="PK683_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH07057.1"
                     /translation="MIREERNDYIAELYVKTNEFNSFGKEFFLELRDLLKKLSLDRKV
                     KAVIFSSDNEKYFSKGFNPEFFIGKSREEIFEGVSLAVNVSHEALFFSKPVIAAVNGH
                     AFGVGAVLALYSDYKIMIQKKPRISFPESLIGLNFPGVTAYVLTQSVGLKTARELLYT
                     GKMVKAEEAMNIGLIDETALDEKDLLERARKYCRTFKDMAMESVTGLKSGILEQVRHF
                     ASVNLERDIQGLSDAVSSSNGQEGLRSIHENRRPVFK"
     gene            complement(4429..>5123)
                     /locus_tag="PK683_01055"
     CDS             complement(4429..>5123)
                     /locus_tag="PK683_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH07058.1"
                     /translation="SGNTSVVLMDLRGHGLSEGARGDSPSPEQMYQDVKTLIELIKST
                     DSQPVYLGGHSSGGGLVLNYSSWDKKSDPDGYIFVSPELGYKSGTAREDMKEPFAKPR
                     LWVFILSAFSGGNLFGNTAAVYLNYPKEVLKKDPLLIASLTRNTAVALTPDNPPEQFR
                     KLNRRTGLFVGEKDELFVPEKVTAYLILLNKSSEEKSKSAIIKNENHLSIISGIDKAA
                     VSLFNEWVAGKR"
BASE COUNT         1540 a         1046 c          921 g         1616 t
ORIGIN      
        1 catcaggatg agtcctgcag cgatcagtaa atttgttgac ccgctctgaa atcggttcag
       61 caagttcgga gtatccggga agaaatgacc tattccgatt cccagaaaca ttgcggaaaa
      121 aatccataga gtcagatatc tgtccaggaa ggacagtttt ttttctgaat tcattttacc
      181 ttctcttcga tgaatttact gcaatagtcc ctgatttcat ctctggtcct gcggaaagca
      241 actgcaattt cctcctctgt tccttttact ttggaaggat caaagaagtt atgatgaaat
      301 ctttctgctt tggaaggaat ataaggacag ttttcctttg catgatcgca gacagttatg
      361 atgaaatcaa aatttatatt catatattca cttacattat tggaagtatg tcccgaaata
      421 tcaatgcccg cctccttcat atacagaact gccttgggat tcactccgtg agtttcaatt
      481 cctgcactcc atacttttgc cttctcccct gcgaaatgcc taagccatcc ctcggccatc
      541 tgactcctgc agctgttgcc tgtacacaaa accagaatat tcttcatttc cttctcctat
      601 tttgtatatc gcaaatatac gattattata tcaagcagat tttctgcctc tgaatgaaaa
      661 atatgttaca gactgtctgt acagtttatg cagaggaaaa aggtttaccg caaaggaccc
      721 cgatacatct ttatgaaaag atgaatacag aagaaataat aaaaagttta gaagaagaat
      781 tgggaattat ccttattcgg acgcagctgg aaaatgtctt ggatgacgat ttttgttttg
      841 cgaatgaagt ccatatttgc agggggatga taatctcttt gggaatatgc aaattagata
      901 tttccgctct cccaaaatca ctaggtcaag cgattgattt aaaatatttg accatagata
      961 attgtgtaaa tttaattcac tttgaaagtc ttgctaattt aaaagatctg gatggactaa
     1021 ggctttgtaa tttgtcaatt gtcagcctac cgagcgaaat ttcaaattta ccgaatctaa
     1081 aaaaaatata tttgaaatca aattcatttc tggaaagctt taacagcctt gcctatcttt
     1141 ataatcttga agagttatac ttagcagatt tggaaataaa aaagttacca caggaaattc
     1201 gctgcaataa aaaattaaaa aagatagctc ttaatttttc tgaaaatctt tttgattaca
     1261 atgttttgaa atttataaaa aacactttag aatactttga aattactttt gaaaaatact
     1321 ctcgagttcc caagattgta agaaatttta aaaatctgaa agtttttttc atgaagacga
     1381 cttatggttt taaagatatt tctaatttaa aaaaattaag acatctgaaa gagataacat
     1441 tagcaggttt aaaaactgat gcgctgccaa agagtataaa tactctgaaa aatcttgaag
     1501 tacttcggat atatgaagca gatcctatta atgcagataa gctgaaagga ctgaagaatc
     1561 ttaaacattt gataggaaca aaacctccaa aatatgctta cacctttcat aaacttgaaa
     1621 ctctagaggc tgcatcctat gaccttacag atgtatctat gataaaaaat ttaaaaaggc
     1681 tgtcagaact cagtttgcgg gttcatcaag ctgctgaatt gtcggaagaa ttatttacct
     1741 gttccaattt aatttccctt gcaatagagg cgaatcttga atatatccca gaatctgttt
     1801 caaagcttga aaagttggag gaattgacat tgagaaattg tatgaattta aaagacattc
     1861 aagcgattaa taaattgaag aacttacagc ttctgagaat ttttgagctg acaaatagag
     1921 atatcgaaag gcaaattatg ccaagagaaa atctgcattt ggatattcga tacattgatt
     1981 aacttcaaat ctcagtgtct ctgtggaaat acttcaccgg agcgactctg catcagaaag
     2041 gatgcggatt ttccaaaccg tcagtacaat ttcagactaa ctgcccatag ataaaaacgc
     2101 acccgacaga caggtgcagt cttttattta cctatatatc cgttcagttt aaatgacccc
     2161 aaaatgtcta tttcaacttt ctgatataga gttccttttt aactctagcc acagttaccc
     2221 cggactcatc cttgatttct gtctcataga aacgagtggt ctttttttcc tttgcagtaa
     2281 tgtttttgat ttcttccagt tcggcgtcgg aaatatgaaa gagcaccttt acaatccctt
     2341 tccccggttt caaaaactca atagacgcac ttttatccca gaccatataa tcatctccca
     2401 gatttctgat cagaagaaac atataaaaag gatcacacat tgaataaagc gatcctccga
     2461 aatgcgttcc tacataattt gtattggata cagtcagagg catcgagact tccactgtat
     2521 ttttgtctat gatggaagga ctcactgatg ctcctctgta gggaccaaac attcggaatg
     2581 cctcctccat tccgtaattt tcagaaagaa atttccataa tgggaaaaca gaaaattcta
     2641 atgatttttt atttttttcc atatcagtaa ggtttttggc cgttgtagtt tcccggagga
     2701 tcgtaattgc atcctattat catccctgca ttgctgggac aattggcaag ataacatccg
     2761 agttttgttg aattcttcca taccacctga gtgtaatgcc cgcagacttt tcctgaagca
     2821 caggagttgc ttgagtaggt ataattggat ttttccgaat accaggaagt caccgcatcc
     2881 gtcacagtcc agctgctccc gcttccccag aaaagatttt ctccataacc tggatttgtc
     2941 ggtcctgcag tatgactgag ctgacatccg ttatttgcca ggtattctac cttcgatttg
     3001 gaataggctc ctactgtcgc atcccaggaa agactcggca gtcctgcatt ttcctgagtt
     3061 ctcaccttgt tatgctccga tgtgattcct gaaacagctg aatactctgt ggcagatgag
     3121 gaagaggcag atgccaagac ggcgacagct gcaattgatg cattattatt cttttccttt
     3181 tcaggagaga tgatgctgca ctgaatcagg aacatcagac tgaatatagt atatttcata
     3241 aaagatcctt tactcgctct gatttcagat aagattatat actgaatcag gtcaagactt
     3301 tttagtactg ccgcaagtcc tcaccggatg acctgaccgg tttttttaga ggaaagagat
     3361 atgcagtttt tttaatatgt ttttttaaat gtaatattaa tttattatat aatatattat
     3421 ttaataagca caaaattaaa ctattgcgtc aggaacattc tgaaactgcg agggattcat
     3481 cttcgagaca gataaagccc agaatcaggt ctggattact tttttgccat tgctgctgat
     3541 gatttagtga ttatttgaag acaggtcttc tgttctcatg aattgaccta aggccttcct
     3601 gcccattgga agaactgact gcatcggaaa gtccctgaat atccctttcc agattgacag
     3661 aagcaaaatg tctgacctgc tccagtattc ccgacttcaa tccagtgaca gattccattg
     3721 ccatatcctt gaaagtcctg cagtattttc tggctctttc cagaaggtct ttttcatcaa
     3781 gagcagtttc atcaatcaga ccgatattca ttgcttcttc agccttgacc atttttcctg
     3841 tataaagcag ttctcttgca gttttcagac ccacactctg agtcagaaca tatgcagtga
     3901 ctcccggaaa attcagtcct atcagagact caggaaagga aatcctgggc tttttctgta
     3961 tcattatttt ataatcagaa tataatgcaa gaacagctcc tactccgaat gcatgaccgt
     4021 tgactgctgc aatgacaggt ttgctgaaaa acagagcctc atgagagaca tttacagcaa
     4081 gagacacgcc ttcaaatatc tcttctctgc tctttccgat gaaaaactca ggattgaatc
     4141 ctttcgaaaa atacttctca ttgtcagatg aaaaaatcac tgccttgact ttcctgtcga
     4201 gtgaaagttt tttcaggaga tctctcagct ccaggaaaaa ttcttttcca aaggaattga
     4261 attcatttgt ctttacatac agttctgcaa tgtaatcgtt tcgttcttct ctgatcatat
     4321 ctgctgcagt tctcctttga cagtcattta catcaaaggt atagaagact caatctgtca
     4381 agctgagatt cacttcatat ccttgaattc aggaactgcc gcagtatatt atctttttcc
     4441 tgcaacccat tcattgaata aagacactgc cgccttatca atgccgctga taatggaaag
     4501 atggttttca ttttttatta tcgcagattt ggacttttcc tctgacgatt tattcagcag
     4561 aatcaaataa gctgtaactt tttccggaac gaacagctca tctttttctc ccacaaacag
     4621 acctgttctt ctgttcagtt ttctgaactg ctcgggaggg ttgtccggag taagtgcgac
     4681 tgccgtattt cttgtcagag aggctatcag aagaggatct tttttcagaa cctcctttgg
     4741 gtagttcaga taaactgcag ccgtattccc aaagagattt ccgccgctga atgcagaaag
     4801 gatgaatacc cagagtctgg gttttgcaaa cggttccttc atatcctccc ttgcagtgcc
     4861 cgatttatat cctaattcag gcgatacaaa aatataaccg tcgggatcag actttttatc
     4921 ccatgaggaa taattcagca ctaaacctcc tccggaagaa tgaccgccaa gataaacagg
     4981 ctgactgtct gtggacttta tcaattctat aagtgttttt acatcctggt acatctgctc
     5041 aggactggga gaatcgcctc tggcaccttc tgaaaggcca tgtcctctga gatccataag
     5101 aacaacagag gtgtttccgc tct
//
LOCUS       DAOQGJ010000037         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8462_length_5107_cov_3.625297, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000037 DAOQGJ010000000
VERSION     DAOQGJ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8462_length_5107_cov_3.625297"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..>1179
                     /locus_tag="PK683_01060"
     CDS             197..>1179
                     /locus_tag="PK683_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNH07059.1"
                     /translation="MYILNYIIRKTEHFSLIKEMFLIKKPDKKAYEAAQKFKIKDKQS
                     VPTDLQTMKEVLALGHPIIFGTQLFASFDECEENGGFVPMPSPKETGRESHGGHAMLI
                     VGYSDPDKVFIVRNSWGKEWGDKGYCYMPYNYLANEKLTGDCWSLISTAGEKAEKNAK
                     KAWSKDKKKSFANKPKTKTYKPEEYEGDTFYYYFPEDEIWDYSEEEPEDYSSMYDEYY
                     DPEANYEDWDDAELEEYAQEDGDFVPDEDEPEEEVDWDAWEEEPEEEEPVDEEEGAEG
                     ETEDEGGNEEGEEEAEGSDEEGEEPAEGEEELAEDEEPAEGEEPEEEPAEE"
     assembly_gap    1180..1279
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1280..1466
                     /locus_tag="PK683_01065"
     CDS             <1280..1466
                     /locus_tag="PK683_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07060.1"
                     /translation="LLRKNLLRKSLLRKNLLRNQSRNRNQKNLQRSLQANQSRNRSLK
                     LPQKKVKLLNQRVNLIP"
     gene            complement(1442..2080)
                     /locus_tag="PK683_01070"
     CDS             complement(1442..2080)
                     /locus_tag="PK683_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07061.1"
                     /translation="MLNLFFTAVLFFLISFHSVPVNSDELRETKSWNYFAYGGQFTAT
                     DLMPILFRQSTDYKDSYIAVLGANRETDFRIRFFQFETELNAVKHFGEMNHFEGNGLY
                     SARVKNLFYLPMSFAFGEGLSIASENPRLENKSKGIYFENYQFLIQSKGIESRNILNF
                     IMVELDYGLSKSFYDSKIFMRIHHRSGVFGLLCPPDPACGSNFVSYGIKFTL"
     gene            complement(2451..2726)
                     /locus_tag="PK683_01075"
     CDS             complement(2451..2726)
                     /locus_tag="PK683_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07062.1"
                     /translation="MNEKIDKVKDLLPGCRILLRFLHMLVEAFEEQKSLYENEKVSSN
                     RKISALEAEVQRLKKENRRLLSLEDRIKAIETLQIAQAAQAATAARK"
     gene            complement(2924..3424)
                     /locus_tag="PK683_01080"
     CDS             complement(2924..3424)
                     /locus_tag="PK683_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNH07063.1"
                     /translation="MKNKRFTVTAALLITVGAGIGCRYESGSNAPLGKVFFNLQRAFG
                     SKISVSCDTDTPAISTKKISGKVKDVTALSAVYNARVSTDPSVVTTTLTDEAGSFSIP
                     GITSTVTDMTVGVTATGYDTFSDKIKLNCQNSVLTILISKVEVSALGVNPTTLDSSKL
                     DQSNIQ"
     gene            complement(3591..4628)
                     /locus_tag="PK683_01085"
     CDS             complement(3591..4628)
                     /locus_tag="PK683_01085"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNH07064.1"
                     /translation="MKEYIKKKIIGGAVFALGFFGTTVFAVAISGTIKTWTSGEVLKS
                     ADLNTTIASLKTAIEGITSSQWTTSGSNISYTSGNVGIGTTSPEGPLTFAGATGQKIE
                     LYSNATNGNEFGMGVESAELRISTGIGSFMSFYTGGYSVGSEKMRITSGGLVGVGRTP
                     TTHPLEVQGTAGLTTGTAWTNISDERLKEIDGKVSSALESLLKLDPIKYKYNETSGKL
                     FKNADGKSKYGFTAQNVKSVFPEMVITDDKGYLWYNPSGFEAILTSAIKELTHKLRSL
                     ENTADSSVAITQKINSLEKEKSANEKKVSALEAEVQRLKAENRRLLSLEDRIKAIETM
                     QIATAAQAARK"
     gene            complement(4668..>5107)
                     /locus_tag="PK683_01090"
     CDS             complement(4668..>5107)
                     /locus_tag="PK683_01090"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNH07065.1"
                     /translation="LHVVGTTLAAAWSTSDERYKKNIQSIPNPTEKILKLRGVNYEWK
                     REEYKDLKFKAGQDFGFIGQEVEKVLPETIFKDEKGYLYVSYQTILPVLVEAFKEQKS
                     ANEKKVSVLEAELHRLKTENRRLLSLEERIKTLETTQMSYSRK"
BASE COUNT         1452 a         1083 c         1012 g         1460 t
ORIGIN      
        1 atatctgtta aggacaatta tagaaatttc aaataaaatt cctgaattaa aaaatgaaat
       61 aaataaattt tattataaaa tcagatttga atcgcttgtt tatttaaaag ataaattaaa
      121 taaacctgaa ttttatgatt ttttaaaatc tgttgtattg tcagaaagat tttcttatga
      181 tttaaaaata agagatatgt atattttaaa ttatataata agaaaaacag aacatttttc
      241 tttaattaaa gaaatgtttt taattaaaaa acctgataaa aaagcttatg aggctgctca
      301 gaaattcaaa atcaaagaca agcagtctgt tcctacagat cttcagacaa tgaaggaagt
      361 tctggcactc ggccatccga ttattttcgg aactcagctt tttgcatctt ttgatgaatg
      421 tgaagaaaac ggtggatttg ttcctatgcc ttctcctaag gaaacaggaa gagaatctca
      481 tggcggtcac gcaatgctta tcgtcggtta ttctgatccg gataaagtgt tcatcgtaag
      541 aaactcctgg ggaaaggaat ggggcgacaa aggctactgc tatatgcctt acaattatct
      601 tgcaaacgaa aaactgactg gcgactgctg gagtctgatt tcaactgcag gcgaaaaggc
      661 agaaaagaat gccaaaaaag cctggagcaa agataagaaa aaaagttttg ctaataaacc
      721 aaagacaaaa acttacaagc cggaagaata tgaaggcgac acattctact actatttccc
      781 tgaagatgaa atctgggatt attctgaaga agaaccggaa gactattcct ctatgtatga
      841 tgagtattat gatccagaag ctaactatga agactgggat gatgcggaac tggaagaata
      901 tgctcaggaa gacggcgatt tcgttccaga tgaagatgaa cccgaagaag aagtagactg
      961 ggatgcttgg gaagaagagc ctgaagagga agaaccagta gacgaagaag aaggtgctga
     1021 aggcgaaact gaagacgaag gcggtaacga agaaggcgaa gaagaagcag aaggcagcga
     1081 tgaagaaggt gaagaaccgg ctgaaggcga agaagagtta gcagaagatg aagagcctgc
     1141 tgagggtgag gagcctgaag aagaacctgc tgaagaagan nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnc ctgctgagga agaacctgct gaggaagagc ctgctgagga
     1321 agaacctgct gaggaaccag agccggaacc ggaaccagaa gaacctgcag aggagcctgc
     1381 aagcgaacca gagccggaac cggagcctga agctcccgca gaagaaggtg aagctcctta
     1441 atcagagggt aaatttgatt ccataagaaa caaaatttga tccacaggca ggatcgggag
     1501 gacatagaag tccgaaaact cctgacctgt ggtgaatcct cataaagatc tttgagtcat
     1561 aaaacgactt ggaaagtcca tagtccagtt ccaccataat aaaattcaga atattcctgg
     1621 attcaattcc ttttgactga atcagaaact gatagttttc aaagtatatt cccttcgatt
     1681 tattttccag tctgggattt tcagacgcaa tagaaagccc ttctccaaaa gcaaaactca
     1741 tcggcagata aaacagattt ttcactctcg ctgaatagag tccgttccct tcaaaatgat
     1801 tcatttctcc aaaatgtttc accgcattca gctcagtttc aaactgaaag aaccgaatcc
     1861 tgaaatcagt ttcgcggttt gcaccaagaa ctgcaatata agaatcttta taatcagtac
     1921 tttgacgaaa caggatcggc atcagatcgg tcgcagtgaa ctgccctccg taggcaaaat
     1981 agttccagga tttagtttct cgcagttcat ctgaatttac aggaacagaa tggaaagaaa
     2041 tgagaaagaa aagaacagcg gtaaagaaaa gattcaacat atcagattct ccgaatattg
     2101 cagataccgc cagtaaaagg cctgcggtaa aataggaaat ctttattcct gtaacttgag
     2161 aaagaaaacc ttgcagtgaa tacttacagg atatttttaa tacagattca tatacagtct
     2221 ggaacagctt tctggcggag gttcagactc tgcaaaggcc tgtcataccg agtccgtcga
     2281 actatgacat ccttcgcgtg cctctctgcc gccttacttc attgtcagaa aggatttgcc
     2341 aatggggacc ggtcatcgag taacagcttc cgaggctgtg tatgcttcga ttcacaggtt
     2401 taaaagctgt tactcagcac aggtcgagat gccggagaga ggtgaaactc tcatttcctt
     2461 gccgctgtcg ccgcttgcgc cgcttgcgct atctgcaaag tctcgattgc tttgattcta
     2521 tcttcaagtg aaagaagcct tctgttctct ttcttcagtc gctgaacttc tgcttccaga
     2581 gcagatatct ttctattgct ggatactttt tcattctcat ataatgattt ctgctcctcg
     2641 aatgcctcta cgagcatatg cagaaatcta agcagtatcc tgcagccagg gagcagatct
     2701 ttgactttgt caattttctc attcaaaaat atcaaggcac ctcctctggt acggctgatc
     2761 aattgatcga aaatttttca tattgtattt tctatatcat tagagttgtt gaaaaattag
     2821 tttcaacaac tctattttta atttttctaa aaaaacctgc attgcccgct ccattggaaa
     2881 aaatcttcca atggagagat atctttgata ttcttcagcc agatcattgt atattagact
     2941 gatctaattt agaactgtct aatgtagtcg gattgactcc cagagcagaa acttccacct
     3001 tgctgatcag aatcgtcaga accgagttct ggcagttcag tttgatcttg tcgctgaaag
     3061 tgtcgtatcc agtcgctgtc actccgacag tcatatccgt tactgtggaa gtgatgccag
     3121 gaatgctgaa acttcctgcc tcatcagtca gtgtagtcgt gacaactgaa ggatcagtag
     3181 atactctcgc attatagact gcactcaacg cagtcacatc cttgaccttt ccggaaatct
     3241 ttttcgtgga aattgcagga gtgtccgtat cgcaggatac agatatcttg cttccgaatg
     3301 cccgttgcag attgaaaaaa acctttccca aaggcgcatt gcttcctgat tcgtatctgc
     3361 atccgatgcc tgctccgact gtaatcagca gagccgctgt aactgtaaat ctcttgtttt
     3421 tcattagaat ctccttaaaa ttccatatgt cttttgatgc tttgcagctc gcttactccc
     3481 gcacctgtca tttcgaacag cagccggttg gtgagcttgc cgaaccgcaa agtctgcagt
     3541 gagaaatctt acatcattag gagcggttga cgttctcaca ctgatcccca ttatttcctc
     3601 gccgcttgcg ccgctgtcgc tatctgcata gtctcgattg ctttgattct atcttcaagt
     3661 gaaagaagcc ttctgttctc tgccttcagt cgctgaactt ctgcttccag agcagatact
     3721 ttcttttcat ttgctgattt ttctttttcg agagagttaa tcttctgcgt aattgcaacg
     3781 gatgagtccg ccgtattctc cagtgacctg agtttatgtg tcagttcctt aatcgcagat
     3841 gtcaagattg cttcaaaccc agatggatta taccataagt aacctttatc atctgttatc
     3901 accatctccg gaaaaacaga tttcacattc tgggcagtaa agccgtattt gctttttccg
     3961 tcggcattct tgaacagttt accgcttgtt tcattgtatt tatatttgat tggatctaac
     4021 tttaataaac tctccaatgc agaggaaact tttccgtcta tctctttcag tctttcatct
     4081 gatatgttag tccacgcagt acctgtcgtc agtccggcag taccctgtac ttccaaagga
     4141 tgagttgtcg gtgtacgccc taccccaacg agaccgcccg atgtaatccg cattttttca
     4201 gaaccaacag aatacccgcc tgtatagaag gacataaaac tgcctatccc tgttgaaatt
     4261 ctcaactctg cggattctac acccattcca aattcatttc cattggtggc atttgaataa
     4321 agttcaattt tctggccggt tgcacctgca aatgtcaacg gaccttccgg actcgttgtc
     4381 ccaatcccca catttccgct ggtataagat atattgcttc cactcgtagt ccactgagaa
     4441 gaagtgatac cttctattgc ggttttcaaa ctcgcaatcg ttgtattcaa gtcagcactt
     4501 ttcagaactt ctccggatgt ccaggttttg atcgttccgg atatggcgac tgcaaagacc
     4561 gttgtgccga aaaagccgag ggcgaatact gctcctccga tgattttctt tttgatatat
     4621 tctttcatta ttctctcctt gatctgttca gttttctgca ctgcagttca tttccttgaa
     4681 taagacatct gtgttgtctc aagcgttttg attctttcct ccagtgataa aagccttctg
     4741 ttctcagtct tcagtctatg caattctgct tccaaaacag atactttctt ttcatttgct
     4801 gatttctgct ccttgaacgc ctccacgagc acaggaagaa ttgtctggta cgaaacatag
     4861 agatatcctt tctcatcctt gaaaatggtc tcaggcagaa ccttctccac ttcctgaccg
     4921 atgaaaccga aatcctgacc tgccttgaat ttcagatcct tgtattcttc ccttttccat
     4981 tcatagttca ctcctctaag tttcaaaatc ttctctgtag gatttgggat actctgtata
     5041 ttctttttat accgttcatc cgatgtgctc caggcggccg ccaatgtagt gccgacaacg
     5101 tgcaggt
//
LOCUS       DAOQGJ010000038         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8463_length_5107_cov_3.051069, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000038 DAOQGJ010000000
VERSION     DAOQGJ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8463_length_5107_cov_3.051069"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PK683_01095"
     CDS             <1..1117
                     /locus_tag="PK683_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07066.1"
                     /translation="RATNTYSLSKFQAMIWTITLLGSYFYIAVSHGLLLGNGVIPDFN
                     ASLIGLLSISYGGMISANGLGTARPKNELIRVPPRFSNLFSSGSEVDIARLQLFGFTI
                     VAVLIYIYNLIISNPLNGMPDIPTTLLGLLGASHSGYLGGKLMGNKTVISMIKPHHVP
                     GHKYGFYLHILGAGFTERTKVIIGSNSEPLEAEFISPSALGVRVPSFSSFGKQKITLI
                     PPGGSAVTADDCFEVITVDPYEITHGEEREIVIKGSDFVPKMTVEVYGQSLDPVEATS
                     VEYVDSETIKCTLPANLGTGEKWLTIKFPDKREDIDVEACLIVLAPLDSSTDEEEEDS
                     SEESVTEKKTVSKSSAKKKGKPSEEGDFMEFEINSES"
     gene            complement(1451..1930)
                     /gene="rfaE2"
                     /locus_tag="PK683_01100"
     CDS             complement(1451..1930)
                     /gene="rfaE2"
                     /locus_tag="PK683_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNH07067.1"
                     /translation="MNSLEKIQSKIVSFEDAFKFRKNISSKIVFTNGCFDILHRGHVN
                     YLAQAKSLGGFLWVGINSDDSVRKLKGPSRPVNSEQDRAFVLSALESVDAVTLFPHDT
                     PVELLLQIRPEIHTKGGDYVKEKLIEYETVCSFGGEVIILPFVEGQSTTSIIQRMVK"
     gene            complement(1927..2934)
                     /gene="rfaE1"
                     /locus_tag="PK683_01105"
     CDS             complement(1927..2934)
                     /gene="rfaE1"
                     /locus_tag="PK683_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNH07068.1"
                     /translation="MKVSLNKVQKALDTFSSLRILVLGDLILDEYLMGDVTRISPEAP
                     VPVLLVKKEKITLGGSGNVVKNLRALGIDVGVFARVGTDEKSETAKKLLSETGCSSEY
                     SCLTENSEIPTILKTRVIAANQQICRIDKEEILPLTAEEEKNILDRLDYLLSQCGAVI
                     LSDYEKGYLRPQFAQKITELCHERKVFVSVDPQISNFFYYKKAGIMTPNHHEAGNALG
                     RKIYGEKETEAACREIADRLDMESMMITRGEKGMCLYVRKTDSFHHIPTVAREVFDVT
                     GAGDTVISLYTAFRASGFTEVESALISNAGAGIVVQKLGAAAVSPDELLSSLRFLNNL
                     E"
     gene            complement(2918..3547)
                     /locus_tag="PK683_01110"
     CDS             complement(2918..3547)
                     /locus_tag="PK683_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07069.1"
                     /translation="MKRPEVQAGDIYSLDRIFLSPLENRESWSFQELRNRTFSFYSFF
                     LMRALLHSELLGSRIELDSLSLKDRQEISRLGMKNGFEKLVEKIFIPEFAVKGSSEET
                     VPQDIIQNIHPEKSFSSFFSDRKSMNISAGGILESGRFDFYKITEHLFRIYSLEKKTG
                     FMYLRSSANSYLYFLNPENKKRIFYAQAREEMNLPFAFAYIETENESIS"
     gene            4169..>5107
                     /locus_tag="PK683_01115"
     CDS             4169..>5107
                     /locus_tag="PK683_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07070.1"
                     /translation="MKLRPSQIYLIFSIGAASLSSVYYRWVSDDAFISFRYAKNLIDG
                     LGLVYNRGEAVEGYTNFLWTMLLSAGMYLGYDPLRISQALGILFYLSTVYILYRVSLR
                     LFPRPESGWPAFRILPLASLCLAVQTHAAIFATGGLETSLFGFLLALGSSFLLITKKW
                     ESLMIGLSALMLSAFTRPDGLLFYAFGSAYVFLFHFQKDKIVEKIIKFIFMQIPFLVF
                     YLPYWIWKWDYYGYIFPNTYYAKSADSWYISQGIQYILLYFSAYYVFLFIPGILIYVW
                     ISKYYPVISGLKKSDPLKERKLKKEITERTGITSAVF"
BASE COUNT         1415 a         1144 c         1079 g         1469 t
ORIGIN      
        1 cagggctaca aacacataca gcctttccaa gttccaggca atgatctgga caattactct
       61 tctcggaagt tatttctaca ttgcagtcag ccacggactt ctgctgggaa acggagtcat
      121 ccctgatttc aacgcaagtc tgatcggtct tctttccatc agctacggag gaatgatctc
      181 tgcaaacgga ctgggaacgg caagaccgaa aaatgaactg atccgagtcc ctccgagatt
      241 cagcaatctg ttctcttccg gaagcgaagt ggacatcgca agacttcagc tcttcggatt
      301 cacaatcgtt gccgtactga tttatatata taatctaatt atcagcaatc ctctgaacgg
      361 aatgcccgac atccccacta ctctccttgg acttctgggt gcaagccatt caggatatct
      421 cggcggaaaa ctgatgggaa acaagactgt aatcagcatg ataaaacctc atcatgtgcc
      481 cggacataag tacggatttt atcttcatat tctgggcgca ggctttacag aaagaacaaa
      541 ggtgatcata ggaagcaaca gcgaaccgct cgaagctgaa tttatcagtc cgagtgccct
      601 cggagtcagg gttccttcct tcagttcatt tgggaaacag aagatcacac tgattcctcc
      661 cggcggttct gcagtgacag cggacgactg ttttgaagtc atcacagtgg atccttatga
      721 aatcactcac ggtgaagaaa gagagattgt catcaagggt tctgactttg tgcctaagat
      781 gacagtggaa gtgtacggac agtccctcga tcctgtcgaa gcgacttctg ttgaatatgt
      841 tgattcagaa acgatcaaat gcacgcttcc agcaaatctg ggaacagggg aaaaatggct
      901 tacaatcaaa tttccggaca agagggaaga cattgatgtg gaagcctgcc tcatcgttct
      961 cgcgcctctg gacagcagca ccgatgaaga ggaagaagat tcatctgaag aatctgtaac
     1021 tgaaaagaag acagtatcca aatccagtgc taaaaagaaa ggcaaacctt ctgaagaagg
     1081 agattttatg gagtttgaaa tcaattcgga atcctgatga gatatctcat cgctgtcctg
     1141 atcatgatat tttcatttgc agaatgcagt gaagaaaaca acactgcata ctgcagaaga
     1201 aaaaaagcgg aaaaggaact ctgtatggct tttttttcag taactgtgca aaaggaaatg
     1261 cagacaacct gcagggatgc tctgcagttt ttttatgttt gacagtacct gcgacaatcg
     1321 acctgcaaaa tgcaaggcac accgtccaat agcactgttt ccaaaggaaa gcagggaaat
     1381 aaagaaaaat cagtaaattg tgccggacct ttccgtgtat gacatcagtt ctttcgaaat
     1441 ttcctgaatg ttacttaacc attctctgta taatcgatgt tgtggactga ccttccacga
     1501 aaggaagaat gatgacttct cccccgaagg aacagactgt ctcatattcg atcagttttt
     1561 ccttcacata gtctcctccc ttcgtatgaa tctcaggacg gatctgaaga agaagttcta
     1621 cgggagtatc atgaggaaat aatgtaactg catccacaga ctccagtgca gagagaacaa
     1681 atgctctgtc ctgttcagaa ttcactgggc gggaaggacc tttcagcttt ctgactgaat
     1741 catctgaatt gattcccacc cagagaaatc ctcccagaga ttttgcctga gccagataat
     1801 tcacatgtcc tctgtgcaga atgtcgaagc atccgtttgt aaatacaatc tttgaagaaa
     1861 tattttttct gaatttaaat gcatcttcaa aggatacgat cttggactgt attttttcga
     1921 gtgagttcat tccaggttgt tcagaaaacg cagagaggaa agaagttcat ccgggctgac
     1981 tgcagctgcc ccgagcttct gcacaacaat tcctgcacct gcgttggaga tcagtgcaga
     2041 ttccacttcg gtaaagcctg aggcacggaa tgcagtgtag agagagatga ccgtgtctcc
     2101 tgcccctgtc acatcgaaga cctctcttgc cacagtaggg atatgatgaa aactgtcagt
     2161 ctttctgaca taaagacaca ttcccttttc tcctctggtg atcatcatgg attccatatc
     2221 cagcctgtct gcaatctctc tgcatgcagc ttctgtttcc ttttctccgt aaatctttct
     2281 gccgagagcg tttcctgcct cgtgatgatt cggagtcatt atgcctgctt ttttataata
     2341 aaagaaatta ctgatttggg gatccaccga aacgaatact tttctttcat ggcagagttc
     2401 cgtaatcttc tgtgcaaact gaggtctcag atatcctttt tcatagtcgg aaagaatcac
     2461 agcaccgcac tgagaaagaa gataatccag tctgtccaga atatttttct cttcctctgc
     2521 agtcagagga aggatctctt ccttgtcgat cctgcatatc tgctgatttg ctgcaatcac
     2581 tcttgtcttc agaattgtag ggatctcact gttttccgtc agacaggaat actcggaact
     2641 gcatccggtt tcagaaagaa gtttttttgc tgtttccgat ttctcatccg ttcctactct
     2701 ggcaaacact cccacatcaa tcccgagcgc tcttaagttt ttgaccacgt taccggaacc
     2761 gcccaaggtg atcttttcct tttttacaag aagcacaggt acaggggctt caggagaaat
     2821 ccttgtgaca tctcccatca ggtattcgtc gaggatcaga tctccgagaa caagaatgcg
     2881 gagagaggaa aatgtatcga gggctttctg aaccttgtta agagatactt tcattttctg
     2941 tttctatata agcaaatgca aaaggaagat tcatttcttc gcgggcctga gcatagaaga
     3001 ttctcttttt attttcaggg ttgagaaaat acagatagga gttggccgaa cttctgagat
     3061 acatgaatcc tgtcttcttc tccagagaat atatacggaa aagatgttca gtgattttat
     3121 aaaaatcaaa acggcctgat tccagtatcc ctcctgcaga aatgttcatg ctctttctgt
     3181 cggagaaaaa agaagagaag gatttttcag gatgaatatt ctgaatgatg tcctgaggca
     3241 cagtctcttc agaacttccc ttgactgcaa attcgggaat gaaaattttc tccaccagct
     3301 tttcaaatcc gttcttcatt ccgagcctgc tgatctcctg cctgtctttc agagagagac
     3361 tgtcgagttc aatcctgctt ccgagaagtt ctgaatgaag cagggctctc atcagaaaaa
     3421 aactgtaaaa tgaaaatgtc ctgttcctga gttcctgaaa actccaggac tccctgttct
     3481 ctaaaggaga cagaaaaatt ctgtcgagcg aataaatatc acctgcctga acctcaggtc
     3541 ttttcaagag cggtggagat tgtactgtca ttctgtacag attatcgagt acaatgtctt
     3601 ttttgtttaa ctgaatcttt gcgatgaata ttcagagatg ggacataatg cattgcaaat
     3661 ttaaaaataa tactgccgga tgcaaaaaaa accttcggag aaagaagtgt tcagccgaac
     3721 gtcagaatca ctgaaccgga aatactgcat actttatgat aattatattt taattcattt
     3781 acatttttca tgaaccgcaa gcggagaggc aaaaatctga aaagcagtta agcattcaga
     3841 agactgctgt cggaaggtca gaacaagact gcagttctgc agaaacagat tccggctgtc
     3901 ttgagctgaa ctgaagctgc tgtgaatgcc aagtgcattc cgctgaattc cctgaaaacc
     3961 agacctgcgg aaaaaacata agaacattgt catatgagcc ggcctgccct gagtttattg
     4021 aagggacagc agcatttact gagtatttca ggcgtttcag cagaggtctt tctcagaatt
     4081 ccgaggtgca aattcacttg ccatattttt aatctgtatt taggctgatc ctgtcatgtc
     4141 atatatggtg cagaggattt ctgctttttt gaaattaagg ccttcacaga tttatctgat
     4201 tttttcaatc ggtgccgcat ccctttcctc tgtctactac cgctgggttt ccgatgatgc
     4261 tttcatttcg ttccgctatg ccaaaaacct gattgacgga ctcggactcg tttacaatcg
     4321 gggagaggct gtggaaggct ataccaattt tctctggacg atgctcctgt ctgcagggat
     4381 gtacctggga tatgatcctc tgagaatttc tcaggcactg ggaatccttt tctatctgtc
     4441 cactgtatac attctgtaca gagtcagtct ccgtcttttt cccagaccgg aatcaggctg
     4501 gcctgctttc aggattcttc ctctcgcttc cctatgtctt gcggttcaga cccatgcagc
     4561 catctttgcc acaggcggac ttgagacttc cctcttcggc ttccttctgg ctctcggctc
     4621 ctcatttctg ctcattacca aaaaatggga aagtctgatg atcggtcttt ccgcactgat
     4681 gctctctgca ttcaccagac cggacggact tctgttttat gcattcggtt ccgcttatgt
     4741 atttctcttt cattttcaga aagacaagat tgtggaaaag atcatcaagt ttattttcat
     4801 gcagatcccg ttcctggttt tctatcttcc ttactggatt tggaaatggg actattacgg
     4861 atacattttt ccgaatacat actatgcaaa atccgcagat tcctggtata tttcacaggg
     4921 gatacagtat attttattgt acttcagcgc atactatgta tttctgttca ttccgggaat
     4981 actgatttat gtctggattt ccaagtacta tccggtcatt tccggactga agaaaagcga
     5041 tcccctgaag gaaaggaaac tgaaaaaaga aatcactgaa agaacaggaa tcacttcagc
     5101 cgtattc
//
LOCUS       DAOQGJ010000039         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8464_length_5107_cov_2.772367, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000039 DAOQGJ010000000
VERSION     DAOQGJ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8464_length_5107_cov_2.772367"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1542
                     /locus_tag="PK683_01120"
     CDS             298..1542
                     /locus_tag="PK683_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH07071.1"
                     /translation="MKQKSIFGNIVTHEKEFKGTVVFDSETGLILSVENAKRTDALEY
                     SDSCFIFPGFGDVHIHAREDESRKHVYKEDFSTASAAALNGGVVHIADMPNNPVPPVD
                     SHSYSVKRKLADSAPIHITLYAGIGPETSPLSERVPYKAFMGPSIGELYFETNADLEK
                     AIERYRGCDVSFHCEDPEILRNSKSAEKHEDRRPKQAEVLATDFALYLIEKYGLNGKL
                     CHYSTKDGLQKIISARKRGVRVLCEVTPTHLFFDREILNEENSFWFQMNPPLRDREDR
                     LELIEAVRRGDIDLLATDHAPHTVEEKKQGISGISQLDTYSLFATHLILNEKIPKTRI
                     AEIAGKNPGDFVRPYLPSRFGKGFGYIEPGYSASFTILNLTEGTVFKKENIKSRSGWS
                     PFENHRFPGSVQAIYYHGTEIE"
     gene            complement(1543..3480)
                     /locus_tag="PK683_01125"
     CDS             complement(1543..3480)
                     /locus_tag="PK683_01125"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07072.1"
                     /translation="MKNFFEKLIRQGSEHHTGNETNLKYNAVSNQILLIFIGGILLFL
                     IYSFTASIYLHSESLDFRKYYSYLLPPVLLMLSILLICFFLKNRTGDNVLYLSIAFIS
                     GIAYLAYMSIAVGLSFYAFLLYFTLIPLPFFVFGRERFRMIIAVESILCLFLFTVVWY
                     RSHFPPLVFFPEHIASVTNYALIFLTALILMTCSYYLWNETILSEEKLFRERKKTEEA
                     LASLNVLKTQQDGDYFLTSLLTAPLNSVNVQSENAEAEIFLKSKKQFSFKNREKELGG
                     DLNIADSVTLAGKKYIVFLNGDAMGKSMQGAGGALVLGAAFKSLIIRANTASDETVHP
                     EIWLKQTFLELEQIFETFNGSMFVSAVIGLMEENTGFVYYINAEHPFLALYRDGHAEY
                     IENELVHRKLGTAGFNNTVRVKFFQMQNGDSLISGSDGRDDILIDTENGKEMNIDDSL
                     FLRAVEKSAGDLNLLYQALSEYGEITDDLSFLKITYRGKTVEHSSRFQGEHKEIIYNA
                     LREKNSRLYNEALNLFKLALDTLSDNLFLLKQIAVVSLRVGNFEDALAHSLKVVDAAP
                     SETRFLFYSSYAYYRLGEMQKAAYYAERVFLREPENLKNIIHLARLHQMMNNSRQAEY
                     YSSAGLKLDPENKALLKLLES"
     gene            complement(3551..>5107)
                     /locus_tag="PK683_01130"
     CDS             complement(3551..>5107)
                     /locus_tag="PK683_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07073.1"
                     /translation="ILKKGDHYYAEAEGRTDENSVTVLESAPVQGKLPSGILNFVQST
                     KIPVILYNALEHSIYGKDPYIQMNEIKSVICQAILKNNDIFGIIYLENDILENVFTKE
                     RLQMIEMISGQIMISIENAVYIAEIEKSRKEAVTANRIKTDFIANVSHEIRTPMNGIL
                     GMKSLLESTPLNSEQRDYLNSIAVSAESLITIINDILDLSKIESGKFELEYIPFSLPG
                     TVTDIINLFRPKAAEKKIELKFELSPDIPEIIISDVTRLRQIILNLIGNSLKFTPDGF
                     ISLSVRKLSEKDHTALLSFSVQDTGVGIPKDKLPSLFNPFSQADSSISRKFGGTGLGL
                     AISKKLCIRMGGDISVETKEGKGSIFTFTVEVKLPEENEKKIYNTNTDQFAPVKNSLR
                     IMLVEDNLINQKIAEKLLEKMGFSVSAKAFNGLEAIRYFQNADFDLIVMDVQMPEMDG
                     IQATYNIRNNFPARVQPVIIAMTANAMSGDREKYISSGMDDYISKPISQKELYEKMIH
                     WNSAIIHRKK"
BASE COUNT         1403 a         1072 c         1102 g         1530 t
ORIGIN      
        1 gaccggaacc tccgagttcc ggatgactga actgtgccat tgtgccgtgt ccgttgatca
       61 atgcctgaag cattgtcata actgcatccg tactgatata aaactgtcct gcgatttcac
      121 tgattctctg ctgaccctga ggtgttagcg gctgcataag tttctcctgt gttcaaccct
      181 tgggctgctt aacctatttt aagtagtcac tctgcagtgt caacattata aataaggtat
      241 tttactttca tctgaatatg ctttacaaaa aactgctgat cgcagaatgg tcagtcaatg
      301 aaacagaaat ccatattcgg caatatcgtc actcacgaaa aagaatttaa gggaacagtt
      361 gtattcgatt ctgaaacagg tctgattctg tccgtagaaa atgcaaaaag aactgatgct
      421 ctggaatatt cggacagctg ctttatattt cccgggttcg gagatgtgca tattcatgca
      481 agggaagatg aaagccgtaa gcatgtttat aaagaggatt tttcgactgc atctgctgct
      541 gctctgaacg ggggagttgt tcatattgca gatatgccga acaatcctgt gccgcccgta
      601 gacagtcatt cctattcagt caaaaggaag cttgcagatt ctgcccccat tcatatcact
      661 ctgtatgcag gaatcggtcc ggagacaagc cctctttcag aaagagttcc ttacaaggca
      721 tttatgggac cttccatcgg cgagctgtat tttgaaacaa atgcagatct ggaaaaggcg
      781 attgaacgtt accggggatg cgacgtcagt tttcactgcg aggatcctga gatactcaga
      841 aattcaaagt cagctgagaa gcatgaagac cgacgtccaa aacaggccga agttcttgct
      901 accgattttg cgctctatct gattgaaaaa tacggcttga acggaaaact ctgtcattat
      961 tccacaaagg acggacttca gaaaattatc agtgccagaa aaagaggagt cagagttctc
     1021 tgtgaagtga ctccgaccca cctgtttttt gacagggaaa tcctgaatga ggaaaactct
     1081 ttctggttcc agatgaatcc tcctctgaga gacagggaag acagactgga actgattgag
     1141 gcagtgagaa gaggggatat tgatcttctc gctacagatc atgcacctca tacagtcgag
     1201 gaaaaaaagc agggaatcag cggaatcagt cagctggata catattctct ttttgcaact
     1261 catctcattc taaatgaaaa aattcccaag acaagaattg cagaaattgc aggtaaaaat
     1321 ccgggggatt tcgtaagacc ctatctgcct tcaaggttcg gaaaaggatt tggatatatt
     1381 gaacctggat actctgcttc ttttacaata ctgaatctta cggaaggaac tgtttttaaa
     1441 aaggaaaaca tcaaaagcag aagcggctgg tccccctttg aaaatcaccg gtttcccgga
     1501 tctgttcagg caatatatta tcatggaacc gagattgaat aatcaggatt ccagaagctt
     1561 cagaagagct ttgttttccg gatccagttt cagaccggct gaagagtagt attctgcctg
     1621 tctggaattg ttcatcatct gatgaagtct tgccagatgg atgatattct tcagattttc
     1681 cggttctctc agaaataccc gttccgcgta gtaggcggcc ttctgcattt cgcccagtct
     1741 gtagtaagca taggatgagt agaacagaaa tctggtttcg gagggtgctg catctacaac
     1801 tttaagagaa tgagcaagag catcttcaaa gttgccgact ctcaggctga ctacagctat
     1861 ctgcttcagt agaaaaagat tgtctgaaag agtgtccaga gccagcttga aaagattcag
     1921 tgcttcattg taaagtctgc tgtttttttc acgaagtgca ttatagataa tttccttgtg
     1981 ctctccctga aatctgctgc tgtgctcgac agtttttccc ctgtatgtta ttttcagaaa
     2041 ggacaggtca tcggtgattt ccccgtattc cgagagtgcc tgataaagca ggttcagatc
     2101 accggcagat ttttccactg cacgaagaaa cagactgtcg tctatgttca tttcctttcc
     2161 attttctgta tcgataagaa tatcgtccct tccgtccgat cctgagatca gactgtcacc
     2221 gttctgcatc tgaaagaatt ttaccctgac agtattgttg aatcctgcag ttcccagttt
     2281 cctgtggaca agttcatttt caatatattc ggcatggccg tccctgtaca gtgcaaggaa
     2341 cggatgctct gcattgatat aatatacaaa accagtattc tcttccataa gaccgatgac
     2401 tgccgatacg aacattgatc cgttgaaggt ttcgaatatc tgttcaagtt ccaggaaagt
     2461 ctgcttgagc cagatttcag gatgaacagt ctcatcggag gccgtatttg cacgtattat
     2521 cagactttta aatgcagctc cgagaacaag cgcaccgccc gcgccctgca ttgattttcc
     2581 cattgcatcc ccgttcagaa atacaatata tttttttcct gcaagagtca cggaatcagc
     2641 tatattaaga tcacctccga gttccttttc cctgttcttg aagctgaact gttttttgct
     2701 tttcagaaat atttctgcct ctgcattttc actctgcacg tttacgctgt tcagcggcgc
     2761 agtcagaaga gaagtgagaa aataatctcc gtcctgctga gtcttcagta cattaagcga
     2821 ggccagtgct tcttctgttt tttttctctc tctgaaaagt ttttcttcag acagaattgt
     2881 ttcattccag agataatagg aacaggtcat cagaatcagt gctgtgagaa atatgagagc
     2941 ataatttgtg actgatgcaa tgtgttcagg aaaaaagacc agcggaggaa aatgactcct
     3001 gtaccagacg acagtaaaaa gaaacaggca gagaatcgat tcaacggcaa tgatcattcg
     3061 gaatctttct ctgccgaata caaaaaatgg aagagggatc agagtaaagt aaagcagaaa
     3121 ggcataaaag ctgagtccca ctgcaatgct catgtaggca aggtaggcaa ttccggagat
     3181 gaatgcaata gaaagataca gtacattgtc gccagttctg tttttcagga aaaagcagat
     3241 aagcaggatg gaaagcatca gaaggacagg aggaagaaga taggaataat attttctgaa
     3301 gtccagactt tctgaatgaa gatagatgct cgcagtgaag ctgtaaatga ggaacagaag
     3361 tatcccccct atgaatatca ggagtatctg attggatact gcattgtatt ttagatttgt
     3421 ttcatttccc gtatgatgtt cgcttccctg acgaatcagt ttttcaaaaa aattcttcat
     3481 aactcactgc ctttgatgac agtttctttt ttcctcagcc ggtttgtcga gggataaaaa
     3541 acgaaggcag ctatttcttt ctgtgaatga ttgcagaatt ccaatgaatc attttttcgt
     3601 aaagttcctt ctgagaaata ggcttgctga tataatcgtc cattcccgag ctgatgtatt
     3661 tttctctgtc tcctgacatt gcatttgccg tcattgcaat aatgacaggc tggacgcgcg
     3721 ccggaaaatt gtttctgata ttgtatgttg cctgaattcc gtccatttcc ggcatctgta
     3781 catccatcac aataagatca aagtccgcat tctgaaagta tctgattgcc tcaagtccgt
     3841 tgaatgcttt tgcgctgacg gaaaagccca ttttttcaag aagtttttct gcaatcttct
     3901 gattgatgag gttgtcttcg accagcatta tcctcagact gtttttgaca ggcgcaaatt
     3961 gatcagtatt tgtattgtat attttctttt cattttcttc aggcagcttc acctcgacag
     4021 taaatgtaaa aatagaacct ttcccctcct ttgtttccac gctgatgtca ccgcccatac
     4081 gaatgcagag ttttttggag atggcaagtc ctaggccggt accgccgaat tttctgctga
     4141 tcgaactgtc tgcctgggag aaagggttga aaagcgaggg aagtttgtcc ttcggaattc
     4201 ccactcctgt atcctgcact gaaaatgaaa gcagtgcagt atgatccttt tctgaaagct
     4261 tgcgcactga gagagaaatg aatccgtccg gagtgaactt aagcgagttg ccgatcaggt
     4321 tcagtataat ctgtctgaga cgggtgacgt cggatataat gatttcaggt atatcggggg
     4381 acagttcaaa cttaagttca attttctttt ctgctgcctt tggacggaaa aggttgatta
     4441 tgtctgtcac tgttcccgga agactgaaag ggatgtactc gagttcgaat tttcctgatt
     4501 caattttgga aagatccagt atgtcgttga tgattgtaat aaggctttcc gcactgaccg
     4561 ctatgctgtt cagatagtct ctctgttcac tgttcagagg agtggattcg agaagagatt
     4621 tcatcccaag tatcccgttc atcggtgttc tgatttcatg gcttacattt gcaatgaagt
     4681 cagtcttgat cctgtttgca gtgacagctt cctttctgga tttttctatt tccgcaatgt
     4741 agactgcatt ctcaatcgat atcataatct gaccggagat catttcaatc atctgaagtc
     4801 tttcctttgt gaatacattt tctagaatgt cgttctccag atatattatg ccgaagatat
     4861 cattattttt cagtatagcc tgacagatga ctgattttat ttcgttcatc tgaatataag
     4921 gatcctttcc gtatatgctg tgttccagag cattgtaaag tattaccgga atctttgtac
     4981 tctgaacaaa gttcagaatc cctgaaggaa gttttccctg aaccggtgcg gactcaagca
     5041 ctgtaaccga attctcatcc gtccttccct ctgcttcagc atagtaatga tctccctttt
     5101 tcaggat
//
LOCUS       DAOQGJ010000040         5041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8648_length_5041_cov_3.191937, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000040 DAOQGJ010000000
VERSION     DAOQGJ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8648_length_5041_cov_3.191937"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="PK683_01135"
     CDS             complement(<1..2022)
                     /locus_tag="PK683_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07074.1"
                     /translation="MSFRNTFWKLTFRLELFTHTVPLPFAVYMGSAASGLDRENQMIM
                     LMTCFSWAGTTLLLGILWRYVFLKRIFEKIDRIESSVSPETAECQELKLSMMKYPFRE
                     AAVIVFRWLAGAAPAVFHTAYIIGFQKGYPVIQMLPFVILMIGPLSFMAYFFITENTV
                     SELIKKSRLAFCEIPPERIPKFNYYARIILTVFGLVSMPFTLFGYILYSLNRGYFTVE
                     NPAAHIIVLAVMFSFPMLFVAYILGISVRNGISSVNDTLQQVSHGNFSVKLPVSATDD
                     FSVQAYHLNQVISRLSSMYSEISVLNADLDSTVRDRTKSLEEAKAEMESLTELTKNIN
                     SVSSLTDIMSFVMYYLETEYQYKDFWLILPDKDRKMFYTFSFVSAEKSELRDQFFKLW
                     QAPINEKAGIIFEVFSTQEFVFEEFYENNENISHTDRMIFKKSGWKYIFSLPLLIYGE
                     TVGIISVNRPVPPEFITENEKIRIQKFSDQIAGAVSNSILIGSMEQAKAEMESLNELT
                     RNINSVSSLTDIMSFVMYYLETNYHYRDFWMILADREKKKFKSFSFVSPDGNSEKSEY
                     FRNWSRSVGKNAGKIFEAFSEHKTVFCSEAELGEGLHSSDVEIMEKAGWKYLYYIPLI
                     IYGDTIGILSINKPVPPELISEEEKGWLQRVSDQIAGAVQNAELLKEAEE"
     gene            complement(2024..4006)
                     /locus_tag="PK683_01140"
     CDS             complement(2024..4006)
                     /locus_tag="PK683_01140"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNH07075.1"
                     /translation="MISAFHIIRVLIPLILICSFSAGCRKTSDELPQAVSGVLDLKGW
                     NFKSEGILKLDGYWEFYREKFIAPCGTSCTPSAQNNSDRIKVPGNWDNHIAKDKEFGS
                     YRLILRNLTGSGNAVKLNGIYGGYILYINGHSVSQAGTVGRSADESVFELKSELVPLP
                     DGASELELVLHLSNFDHSRGGLRNTLYIGQYKELEELRRRTLETDFFLFGALLMMSLY
                     HLGLYSLRRTDRSALYFAAVCLLFSLRPLFIGEHEIADLLNYPYNIYMIRLEYILLYL
                     TLPFYYLYIREIFPSEFRNRTMNYCMAYLIAVCAVPTVFSAKIFTELLFLFHIGVILT
                     VLTSIAFIILAVIRKREGSLIFSLGILIVSVCAVNDLLYARGAIFTKNYISHGVFFFI
                     FSQAYLLSSRFSKAFRDSEISRSMAEEQRLLAEERKTEIEKLNGMKDEFLANLSHELK
                     TPLTYIYMYSEMLTEPNEQETVIEYGKELLGNAKKLNDYLEDLILMTDIESMKELHLE
                     SVSFASAVEENILSLKSESTQNHVSISYNTEEDDTVLCDRHLFVKMLYAVMKNAILYN
                     REGGSVVLSFQKNAKECIFSCRDTGIGISEKMKERVFEKFFRADSSLKYEKSGVGIGL
                     YLASRIASLHGGRIELDSEEGKGSEFRIIFPLRNYN"
     gene            complement(4019..4384)
                     /locus_tag="PK683_01145"
     CDS             complement(4019..4384)
                     /locus_tag="PK683_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07076.1"
                     /translation="MRKILIIEDQAPMAKILSDILKKENFETFTAINGQAGIDKAVEI
                     RPDMVITDIMMPVKTGWEVLKELRAMPAFEKTPIFIITAKGGASDSKTAADGGASGFI
                     QKPFSPSQIVEEIKKILGE"
     gene            complement(4402..>5041)
                     /locus_tag="PK683_01150"
     CDS             complement(4402..>5041)
                     /locus_tag="PK683_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH07077.1"
                     /translation="VSGLVHDIKNPIGTVKAFAEMLKDSETDHKLREEYADLILRETD
                     RLGDIAYEILDFSKGNIALNLDHHNFAEFLDEIFCFLKIDFEYADIPFSVNCDYEGRL
                     CFDRDRLRRVIVNLANNSREAMSQWKGERKFMISAERQDDTVLIRVEDTGPGIPKTLS
                     PRLFEAFATEGKTKGTGLGLYMSRMIVEQHKGIITYSPYEGSGAVFIIKIPI"
BASE COUNT         1469 a         1167 c          924 g         1481 t
ORIGIN      
        1 ctcttctgcc tccttcagga gctctgcatt ctgaacggca cctgcaatct gatccgagac
       61 tctctgaagc cagcctttct cttcttccga aatcaactca ggagggacag gcttatttat
      121 ggaaagaatc ccaattgtat caccatagat aattaaagga atataatata aatatttcca
      181 gcctgctttt tccataatct cgacatctga agaatgcaga ccttctccca gctcagcctc
      241 agagcagaac actgtcttat gctcagagaa tgcctcaaat attttcccgg cattttttcc
      301 gactgacctg ctccaatttc tgaaatattc agatttctcc gaattgccgt caggcgaaac
      361 aaaactgaag cttttgaatt ttttcttttc cctgtctgca agaatcatcc agaaatctct
      421 gtaatggtaa tttgtctcca gataatacat tacgaaactc ataatatctg tcagggatga
      481 gacggaattt atgtttcttg tcagttcatt caaggactcc atttctgcct tggcctgttc
      541 catactgcct atcagtatgg aattgctgac ggcaccggca atctgatctg aaaatttctg
      601 aatcctgatt ttttcattct cagttataaa ttcaggcgga accggtctgt ttactgaaat
      661 gattccgact gtttctccgt aaatgagaag gggcagagaa aagatatact tccatccgct
      721 ttttttaaat atcattctgt cagtgtgaga tatattctca ttattttcat agaattcttc
      781 aaagacaaat tcctgcgttg agaagacttc gaaaattatt ccggctttct catttatggg
      841 ggcctgccac agtttaaaaa actgatccct gagctctgat ttttcagctg aaacaaaact
      901 gaatgtataa aacatttttc tgtccttatc cggcagaatc agccaaaaat ccttatactg
      961 atattcagtt tccagataat acattacaaa actcataata tccgtcagag atgaaactga
     1021 atttatattt tttgtaagtt cagtcagtga ctccatttct gccttggcct cttccaggct
     1081 tttagtcctg tccctgaccg tactgtcaag atcggcattc agaacactga tttccgagta
     1141 cattgaactc agacggctga tgacctgatt cagatgatat gcctgcactg agaaatcgtc
     1201 cgttgcagac actggcagct tgacggaaaa atttccatga ctcacctgct gaagcgtatc
     1261 gttcacggag gaaattccgt tcctgaccga tattcccaga atatatgcga cgaaaagcat
     1321 aggaaaggaa aacattacag ccagcacaat tatatgcgct gcagggtttt ccactgtaaa
     1381 gtatcccctg ttcagggaat agagaatata gccgaaaaga gtgaagggca ttgaaaccag
     1441 accgaaaaca gtgagaatga ttcttgcata ataattgaat ttaggaatcc tttcaggagg
     1501 gatctcacag aaggctagac ggcttttttt tataagttca gatactgtat tttctgtgat
     1561 aaaaaagtaa gccataaagg aaagaggtcc aatcataaga attacaaaag gaagcatctg
     1621 aatgacaggg tatcccttct gaaatccgat gatatatgcc gtatgaaata ctgccggagc
     1681 cgcaccggca agccatctga acacaatcac agccgcttcc ctgaaaggat atttcatcat
     1741 agaaagctta agttcctgac actccgcagt ttccgggctg actgaagatt cgatcctgtc
     1801 aattttttca aatatccttt tcagaaaaac atatctccag agtatgccga gtaaaagagt
     1861 cgtccctgcc caggaaaagc aggtcatcag cataatcatc tgattttccc tgtccagacc
     1921 ggatgccgct gaacccatat aaaccgcaaa cggaagaggc actgtatgtg taaagagttc
     1981 aagacggaat gtaagcttcc agaatgtatt tctgaatgac attttaatta taattcctta
     2041 atggaaagat gattctgaac tcagatcctt ttccttcctc agaatccagt tctattctgc
     2101 ctccgtgaag agatgcaatc cttgaagcca ggtaaagtcc aatccctact ccgctttttt
     2161 catactttag agaagagtct gcacggaaaa atttttcaaa gacccgctct ttcatctttt
     2221 cagaaattcc tatacctgta tccctgcagg aaaaaatgca ttcttttgca tttttctgaa
     2281 atgaaagaac aactgaacct ccttcacggt tgtaaaggat ggcatttttc atcacagcat
     2341 acagcatctt tacaaaaagg tgcctgtcac aaagcacagt atcgtcctcc tccgtattat
     2401 aggaaatgct cacatggttc tgcgttgact ccgattttaa tgataaaata ttctcctcca
     2461 ctgcagaagc aaaggataca gattccagat gaagttcctt catcgattca atatcagtca
     2521 tcagaataag atcttccaga taatcattca gtttctttgc atttccgaga agttctttcc
     2581 cgtattcaat gacagtttcc tgttcattgg gttctgtcag catctcggaa tacatataaa
     2641 tataggtcaa aggagtcttc agttcatgag aaaggttcgc aagaaattca tccttcatcc
     2701 cgttcagttt ttcgatctca gtctttcttt cttctgcaag cagtctctgt tcctccgcca
     2761 tgcttctgga tatttctgaa tccctaaatg cttttgaaaa tcttgaggaa agaagatagg
     2821 cctgcgaaaa aatgaaaaag aatactccgt gagaaatata attcttcgta aatattgctc
     2881 cccttgcata aagcagatca ttgacggcac agacagaaac aatcagaatg cctaaagaaa
     2941 aaatcagaga accttctctt ttcctgatta ctgcaagtat gataaacgca atgcttgtca
     3001 gtacagtcag aattactcca atgtgaaaaa gaaacagaag ttcggtgaat atttttgcac
     3061 tgaagactgt cggaactgca catactgcaa tcaaatatgc catacagtaa ttcattgttc
     3121 tgttcctgaa ctcagacggg aagatctccc tgatataaag ataataaaac ggaagagtca
     3181 gataaagaag aatgtattcc agccttatca tatatatgtt gtatggataa ttcagaagat
     3241 cagcgatttc atgttcaccg ataaaaaggg gtcttaatga gaaaagcaga cataccgccg
     3301 caaaatagag ggctgacctg tctgttcttc tgagtgaata gagtccaaga tgataaagac
     3361 tcatcatcag aagtgctcca aacagaaaga aatctgtctc caatgttctc cgtctcagtt
     3421 cttccagctc cttatactga cctatgtaca gagtatttct cagcccgccc ctcgaatgat
     3481 caaaattgga aagatgaagg acaagttcca gctcagatgc tccgtctggc agcggtacta
     3541 attcagactt cagctcaaaa acagattcat cagcagatct accgacagtt ccagcctggg
     3601 atacagaatg accgttaata tataaaatat atcctccata aattccgttc aattttaccg
     3661 catttccact gccggtcaag ttccttaaaa tcagccggta tgatccgaat tctttatctt
     3721 ttgcaatatg attatcccag tttcccggaa cctttatgcg gtcagagttg ttctgagcag
     3781 acggagtgca ggaagtaccg catggagcaa taaatttctc cctgtagaac tcccagtacc
     3841 cgtccagctt cagaatccct tccgatttaa aattccagcc cttcagatca agaaccccgc
     3901 tgaccgcttg aggcagttca tctgaagtct ttctgcatcc ggcactgaaa gaacagatca
     3961 gaatcagggg gattaaaact ctgataatat ggaatgccga aatcatcgag cccaaggatc
     4021 attctcccag aattttttta atctcttcca caatctggct gggagaaaag ggtttttgaa
     4081 taaacccgct tgcgcctccg tcagccgcgg tctttgagtc tgatgctccg cccttggcag
     4141 taatgatgaa tataggggtc ttctcaaaag caggcattgc tctcagttcc ttcaggactt
     4201 cccatccggt cttcacaggc atcatgatat cagtgatcac catatccggc cgaatttcca
     4261 cagccttgtc tattcctgcc tgaccgttta ttgcggtaaa tgtctcaaag ttttcctttt
     4321 tcaggatatc cgaaaggatt tttgccatcg gtgcctgatc ttctataatc agtattttcc
     4381 tcataatttt ttctcctgat ttcatattgg tattttaatg ataaagacag caccagatcc
     4441 ttcgtacgga ctgtatgtta tgatgccctt atgctgttcc actatcatcc gggacatata
     4501 cagtccgagt ccggttccct tcgtcttgcc ttctgtcgca aatgcctcga acagacgggg
     4561 agacagggtc ttcggaattc caggacctgt atcctcaacc ctgattagca ctgtatcgtc
     4621 ctgcctttcc gcagaaatca taaattttct ttcacccttc cactgactca ttgcttctct
     4681 ggaattgttc gctaagttca ctatcactct ccgaagcctg tcacggtcga aacagagcct
     4741 gccttcatag tcacagttta ccgaaaaggg gatatcagca tactcaaaat ctattttcag
     4801 aaaacaaaat atttcatcca gaaattccgc aaaattatga tgatccagat tcagtgcaat
     4861 gttccccttg ctgaaatcaa gtatctcata tgcaatgtct cccagtctgt cagtctcacg
     4921 aagaatcaga tccgcatact cttccctcag tttatgatct gtctctgaat ccttcagcat
     4981 ttcggcaaat gccttcaccg ttccaatcgg attctttatg tcatgcacaa ggcctgaaac
     5041 c
//
LOCUS       DAOQGJ010000041         5040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8654_length_5040_cov_3.609228, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000041 DAOQGJ010000000
VERSION     DAOQGJ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8654_length_5040_cov_3.609228"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PK683_01155"
     CDS             complement(<1..561)
                     /locus_tag="PK683_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH07078.1"
                     /translation="MSNSDMEKLEKENAALKEKIAVYEALLQADITAERKNSSEGIAS
                     RSSLYDRIFQMTKDASRTRQIEKELRTSREHFYELIMRLPIGVLVQGPNAEILINNHA
                     ALEMLGISEDQLLGKTSFDPDWNVIHEDGTEFPGPTHPVPVSIRTAKPVRNIVMGVFR
                     PLTKDRVWLLVNAEPFLNEDGTVRDVI"
     gene            complement(798..2402)
                     /locus_tag="PK683_01160"
     CDS             complement(798..2402)
                     /locus_tag="PK683_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HNH07079.1"
                     /translation="MVDINIKLGKIKDSLNKDAKKKLKKLNKHKLRLRNTQLSDYEDI
                     REIMDQVYSNAGGTWSREEFASQLSRFPEGQFCIENNGKLVAAVVSMIIKYSDFGDKH
                     TYDQITSRGYLKNHNPEGDTLYGVDIFVHPKFQGLRLGRRLYDARKELCQKKNLKRII
                     IGGRIPGYKNFALEMSPQQYVELVKRKELHDPVLSFQLANDFHVRKIMTEYFPEDKDS
                     KNYAILLEWLNIYYEEKEKLIGGRKTTVRIGVVQWQMRTVGSPEEFLKQIEFFVDAVA
                     GYKADFVLFPEFFNVPLMAKYNQESPADAIRALAEHTEHLREEMLNMAVSYNINIISG
                     SMPIYSDTRLYNVSYLLRRDGTWDEQFKLHITPDEQSYWGVTGGNTLKIFETDVGKIG
                     ILVCFDVEFPELPRVLAERGMEILFVPFSTDTKNAYQRVRHCAQARAIENECYVAISG
                     SVGNLPNVDNMDIQYAQSAVFTPSDFAFAHDAIAAEATPNTEMTLVVDVDLDLLKELK
                     SHGSVRNMNSRRHDLYRVQWFENSAD"
     gene            complement(2673..3905)
                     /locus_tag="PK683_01165"
     CDS             complement(2673..3905)
                     /locus_tag="PK683_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH07080.1"
                     /translation="MKKKILLFSPPFSGHLHPVLGLGIYLKNTYEIAVVSTSSAMKSI
                     EASGLRGICLLQGKDSFIDEIANPKVQVKNNPVLLYSQLKRNVSLLHTMRKETEDLYV
                     TEKPDIVITDGVLPVPGILAESMGIPWFSILASPCVFESVSGVNAYMGGVKPDSTLKG
                     RIRDSIHRIMIKSFKKFMFFLFSDDLRKLGLENVYRKDGSEAVYSHRKVFAMAMSELE
                     FERKTADTLMLTGPILYTPPVNSESPLFNRGKKHILITIGTHLWFEKDRLKEQIRECS
                     LDLNDFIFHFSYGDKNGTVREIQENFHVYSFVSYEEHLFKYDLVLHHCGAGIMYECIR
                     NGIPSIAYPLDYDQFDNAARLETAGACTVLKNIRKLREAVLNAAENENMKKNCVMLQE
                     KFRNYNAPETIENILSEI"
     gene            complement(4000..4551)
                     /locus_tag="PK683_01170"
     CDS             complement(4000..4551)
                     /locus_tag="PK683_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07081.1"
                     /translation="MKTKIAFLTGQSNPSSTSLSPVQTEFMKKIPVPEELKSYRNFPY
                     TEGSAYSEVNILRASINNARQYLNAGKRSDEYSGMFNKYFKNNDIIIIISGSCGLHLF
                     NSLNLSAEIRQKISIFAYGPTAHRIPECRDIFIVQGRKDWISRLWRLPVNEYAECGHM
                     DYLNQKSVFDSCIRFLKKNGVSA"
     gene            4691..>5040
                     /locus_tag="PK683_01175"
     CDS             4691..>5040
                     /locus_tag="PK683_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07082.1"
                     /translation="MAYGTFKTLEEVMIHFDIEGLNDRFIDELHFESSEIMFNFINKN
                     LSNRRNFISENSICEAVIFPILSIVADKYDLPLWSHTRFDVAPDEGLSGIPDFLIAPV
                     SKTGLSFTDPVICIA"
BASE COUNT         1317 a         1092 c          984 g         1647 t
ORIGIN      
        1 gatgacgtcc ctgactgttc cgtcttcatt cagaaagggc tctgcattta caagcagcca
       61 cactctgtct tttgtgaggg gtctgaatac tcccataaca atgtttctta ccggttttgc
      121 cgttctgata gaaacaggca caggatgagt cggaccggga aattcggttc catcctcatg
      181 aatcacgttc cagtcaggat caaaggaggt ttttcccaga agctggtctt ccgaaattcc
      241 gagcatttca agtgctgcat ggttgttgat cagtatttca gcattcggcc cctggacgag
      301 gacgccgata ggaagtctca taatcagttc ataaaaatgt tctcttgagg tgcgaagttc
      361 tttttctatc tgcctcgtcc gtgatgcatc cttcgtcatc tgaaaaattc tgtcatacag
      421 actgcttctg gatgctatcc cttcagagga atttttcctt tccgcagtta tgtccgcctg
      481 gaggagtgct tcatatactg caattttttc ctttaatgca gcattttcct tctcaagctt
      541 ttccatatca gaatttgaca tacaatattc tcttttactg taattatttg aaattgatgc
      601 ctgctcacct caattctgac ttccgctgaa tttagacact caggaaaact gcctgccgtt
      661 ttaaaaatta tactccgggc atcacagtcc catttggtaa gctgagtcat tatctcataa
      721 ctcttccttc agtcagacag aagacatacc gttgaaataa taagtattcg cacttatctc
      781 aagtcaaata ttatttctca gtctgctgag ttttcaaacc attgtactct gtaaagatca
      841 tgccgtctgg aattcatatt tctcacactt ccgtgacttt tcaattcctt aagcaggtct
      901 aaatccacat ctacgacaag agtcatttcc gtatttggag tagcttccgc agcaattgcg
      961 tcgtgggcaa aagcaaaatc agacggagta aagactgcag actgggcata ctgaatatcc
     1021 atattgtcca cattcggaag gttgcctacg cttccagaga tggcaacata acattcattt
     1081 tcaatggccc ttgcctgtgc acagtgcctc actctctggt atgcgttttt agtatctgta
     1141 gagaacggaa caaaaagaat ttccatccct ctttctgcaa gcactctcgg cagttcggga
     1201 aactcaacgt caaagcatac cagaattcct atcttgccta catcggtttc aaagattttc
     1261 agagtattgc ctcctgtgac gccccagtag ctctgttcat ccggagtgat atgcagtttg
     1321 aactgttcat cccaggtccc gtctcttctc agaagataag atacattata aagtcttgta
     1381 tcggaatata tcggcatgct tcctgatatt atatttatat tgtatgacac tgccatattc
     1441 agcatttctt ctctcagatg ttcagtatgt tctgcgagtg ccctgattgc atcggctgga
     1501 ctttcctgat tgtattttgc catcagaggc acattgaaga attcaggaaa aagcacaaag
     1561 tctgccttat aacctgccac tgcatccacg aaaaactcta tctgcttcag gaactcttca
     1621 ggagatccca ctgtccgcat ctgccactgc actacgccga tcctgacagt tgtctttcgt
     1681 cctccgatca gtttttcttt ttcttcatag tatatattca gccattccag aaggattgca
     1741 taattcttcg aatccttgtc ttccgggaaa tattctgtca ttatttttcg tacgtggaag
     1801 tcatttgcaa gctggaaact caagaccgga tcgtgaagtt cctttctttt gacgagttct
     1861 acatactgct gcggactcat ttcaagtgca aaatttttat atcccgggat tcttcctccg
     1921 atgatgatcc tcttcagatt ttttttctga cagagttcct tccgagcgtc gtaaagacgt
     1981 cggcctaggc gaagaccctg aaatttcgga tgaacaaata tatccacacc gtaaagagtg
     2041 tctccttcag gattatggtt tttgaggtat cctctcgatg tgatctgatc atatgtgtgc
     2101 ttatcgccga agtctgagta ttttatgatc atactgacga ctgctgcaac gagttttccg
     2161 ttgttttcta tgcagaactg accttccgga aatctggaga gctgggaggc aaattcttcc
     2221 ctgctccatg ttcctcccgc atttgaatat acctggtcca tgatctcacg gatgtcttca
     2281 tagtcgctca gctgagtatt cctgagacgg agcttatgct tgttcagttt cttcagtttt
     2341 ttctttgcgt ccttgttcag actgtctttg atttttccga gttttatgtt aatgtcaacc
     2401 ataggtcaat tctctttcat tgaattttag caggtgctca gcctgagggc aagttttatg
     2461 ccttccataa cggctgaaga ctgaattcaa aactataata tactttgctt ttaaaaaagc
     2521 aagaaaagct tgagatgttc ttctgcacac tggcagtgaa tcgtctgcga aatccggttt
     2581 tgcggactcg cagaatctga agcttatgag aatatccgat tttcacgaga tttggaatac
     2641 ccgaatttcc atgatgctct caatataaat attcagattt ctgaaaggat gttctcaatc
     2701 gtttctggcg cattataatt ccggaatttt tcctgaagca taacgcagtt ctttttcatg
     2761 ttttcatttt ctgctgcatt cagaacagct tccctcagtt ttctgatgtt cttcagaaca
     2821 gtgcaggccc ctgcagtttc aagtcttgct gcattgtcaa actgatcgta gtccaaggga
     2881 tatgcgattg aaggaattcc gttccgtatg cattcataca ttatcccggc tccgcagtga
     2941 tgaagaacca gatcatattt gaaaagatgt tcctcataag aaacaaaaga atacacatga
     3001 aaattttcct gtatctctct gactgtcccg tttttgtcac cgtaagaaaa atgaaatatg
     3061 aaatcattca aatccagaga gcattctctg atctgctctt tcagtctgtc cttttcaaac
     3121 cagagatgag ttccaatggt aatcagaatg tgtttttttc cccgattaaa aaggggagat
     3181 tctgaattta caggaggagt gtaaaggatc ggtccagtga gcataagtgt atctgctgtc
     3241 tttctttcaa attccagttc agacattgcc attgcgaata ctttcctgtg agaatataca
     3301 gcctcggatc cgtcttttct gtatacattc tccagaccga gttttctgag gtcatcactg
     3361 aaaaggaaaa acatgaattt tttaaatgat tttatcatga ttctatgaat actgtctctg
     3421 attctgccct tcagtgtgct gtcgggtttt actccgccca tataggcatt gaccccggat
     3481 actgactcga acacgcaggg tgaggccagt atgctgaacc agggaatgcc cattgattca
     3541 gccagaatcc ccggaaccgg gagcactccg tctgtaatta caatatcagg tttttccgtt
     3601 acataaagat cctctgtttc ctttctcatc gtatgcagga gggatacatt tcttttcagc
     3661 tgagagtaca gcaggactgg gttgtttttt acctgaactt tcggatttgc aatttcatca
     3721 atgaatgaat cttttccctg cagaaggcag attcctctca gtccggaagc ttcaatggat
     3781 ttcattgcag aggatgtgct gactactgcg atttcataag tattcttaag atatattccc
     3841 agtccgagaa cgggatgaag gtgtccgctg aaaggcggag agaatagaag tattttcttt
     3901 ttcatctatt tttccttttt tctgaattca gaaacggaga attgaacggc atttaacagt
     3961 gcatctctgc ggtcatcatt ctaatacata gacagaacat taagctgaaa ctccattttt
     4021 tttcaggaat ctgatgcagg aatcaaatac tgatttctga ttcagatagt ccatatgtcc
     4081 gcactctgca tattcattta caggcagcct ccaaagtctt gaaatccagt cctttcgtcc
     4141 ctgcacgata aaaatgtccc tgcattcagg aatcctgtgc gcagtcggac cgtatgcaaa
     4201 aatacttatc ttctgtctga tttcagcact cagattcaga gagttgaaaa gatgaagtcc
     4261 gcagcttcct gatataataa ttattatatc attatttttg aaatatttgt taaacatgcc
     4321 ggaatattca tctgaacgtt ttcctgcatt cagatactgt ctggcattgt ttatgctcgc
     4381 tctgagtatg ttcacttccg agtatgcaga tccctctgtg tagggaaaat tcctgtaaga
     4441 tttgagttct tccgggaccg gaattttttt catgaattct gtctgaacag gactgagtga
     4501 agtgcttgaa ggattgctct gtcctgtcag aaatgctatt tttgttttca ttgcaggaaa
     4561 tacctgatgg cagcattttt cggcagataa gttttgcaag cagaatcctg tcatcagaaa
     4621 aaatgcttga acaagtgctg atagcattga atattgaatc aaatatcctg aattctgcaa
     4681 ggaggggatc gtggcttacg gaacattcaa gactttagaa gaagtgatga ttcattttga
     4741 tattgaaggg ctaaatgacc gatttattga tgaacttcat tttgaatctt ctgaaataat
     4801 gttcaatttt ataaataaaa atctttccaa cagaagaaat tttatcagcg aaaattcaat
     4861 ctgtgaagct gtaatttttc ctatattgag catcgttgca gataagtatg atcttcctct
     4921 ctggtcccac acgcgttttg atgttgcacc tgatgaagga ctcagcggga tacctgattt
     4981 tttaattgca cctgtttcta aaacaggatt aagttttaca gatccggtga tttgcattgc
//
LOCUS       DAOQGJ010000042         5033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8678_length_5033_cov_2.720370, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000042 DAOQGJ010000000
VERSION     DAOQGJ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8678_length_5033_cov_2.720370"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..314
                     /locus_tag="PK683_01180"
     CDS             48..314
                     /locus_tag="PK683_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07083.1"
                     /translation="MYRGGGENHSAAAFTQSLPGLLLYFHLDLFASAGNPSANGKAFK
                     ITDGSSDAYDKSDYHYVMMHPVITVLELENYETVHRLGFLNRTE"
     gene            complement(550..942)
                     /locus_tag="PK683_01185"
     CDS             complement(550..942)
                     /locus_tag="PK683_01185"
                     /inference="COORDINATES: protein motif:HMM:NF025461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4274 domain-containing protein"
                     /protein_id="HNH07084.1"
                     /translation="MKEEDIQTAVSYYKLAKEHNWDDDYEALYGILLDPGCDRGTALM
                     IYWLGKPKYFCQFANKMEVPSYDRENYALLKMIEKKYSTYPAIISYDPKEDIVEYEDV
                     EVKFPIDPVFYEPVKGIISQEELKKRIG"
     gene            1036..1332
                     /locus_tag="PK683_01190"
     CDS             1036..1332
                     /locus_tag="PK683_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07085.1"
                     /translation="MTILISGTLITLALLALANFLEVKFNAFQDASLMKKILIYLGNS
                     VLSSLAMAILGAALGAGVGDESAGDAVIFLFFAGLGIGFVLGLIFTGAWQYFAG"
     gene            1543..4488
                     /locus_tag="PK683_01195"
     CDS             1543..4488
                     /locus_tag="PK683_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNH07086.1"
                     /translation="MNKKSSKVKQIWKEFSFQSGFLAFAENSEAKKSDLKDEERIQLL
                     KAKNLKAEAVFFRRFENRSSLPLVYVYDFTETEISEETKGELHKFIWNSGAVPLFIIF
                     QKAKIEIFNSSKKVDLDENGNPRAVLHKSLSFNETIELAAIFSSLENGTFWDQKENIN
                     YLQSDSAYSSLLKELKTARTRFIKEINVEESFANKLLLISILIKYLEERTGADNKRIL
                     YFKKYNNAKNFCDVIEKNQFLNLLNDLSTQMNGKVFELSASELKILKDKNTNLQPLAE
                     FLSAKLDGKQYVLWPLYSFNHLPVELISGIYEEFLPREKSTVYTPPLLVQLLIDQSMP
                     IHTPRMDLKIFDPACGSGIFLVSAFQRKVDWWKIENYKRTGEFQNPDLTALKKILRER
                     IFGADVKKEAVQIAIFSLSIALCDYLEPTKIWTELRFDDLSEKNILAKNFFTYLKENP
                     ENDFDLIIGNPPFKNFDKEGERDEEALKIHKKELEEKQLTSPQKQIAFLFLKYSMQVL
                     KPNGLLCLVMPSAPLLYNDTLEYRNSILFNKYNVPQILDFTFMDKSLFQKRTSKEEKN
                     KESEKGKGAIVPTAVVFVHNKEPDEKDILHIVIRDTVVAKNKLYFEIDSYDFHYVPKK
                     IAKTKEWIWKVNLLGGGRIYDLIDRLSKLPTLGEYINEKEKKSEWIFNEGYEVGQRGS
                     YKGKFIQRRKHIISEAITEDGKIDINKTELETSESFYRERKEELFLPPLMLFRQVIGE
                     KRLPIALVKNVIPEVDEKYVLFKDGVIGLHVPQEDSSSLEILYKKIHDRHSVYRFILI
                     ATCGQLAQRATEIKKKNIESLPYPENDLKLSKLEKIIIEDVLNFYSKLGGYDSTLDNP
                     VEEKQLFEFGNLLSDILNSVYEKNKKKFHQHKIIITPSFVCSSFQYGSSAISKKEMIE
                     KSSKLEESLEMLVRKNLGTSARINRIILFYHEDKIYLIKPINCRFWLKSIAIQDADEI
                     LMDLIESGY"
     gene            4490..>5033
                     /locus_tag="PK683_01200"
     CDS             4490..>5033
                     /locus_tag="PK683_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07087.1"
                     /translation="MFAESKDEKARQAEVNGLESNQHIDCILVYLEKYIPSFTEKTEL
                     LENEKENKINSKLSMYLNRSIAEENHFPYIFDRENPDSKSPEDIIVSYKAGRREITVF
                     VIECKRLPTPPPEDREKEYVSGKGAGIQRFKELDQGKNFSIAAMIGYIEKENFSYWFT
                     QINSWIEELSNHEKTIWNHKE"
BASE COUNT         1690 a          847 c          981 g         1515 t
ORIGIN      
        1 gtgccgagct tgtcgaggta tgaaaaaccc aatcttttct gggttttttg tatcgaggcg
       61 gcggtgaaaa ccactcggca gccgctttta cacagtctct tcctggtctt ttattgtatt
      121 ttcatcttga cttgtttgca tctgccggaa atccttccgc caatggcaaa gcttttaaaa
      181 taacagacgg aagcagtgat gcttatgata aatcagacta tcattatgta atgatgcatc
      241 cggtaattac agttttggaa ctggaaaact atgaaactgt gcataggctt ggttttctaa
      301 atcgaaccga ataagtaaag aaaacaggat caataatttt ataactccac gtcacattct
      361 gcagactcag tgcaagctcc gcgtgattct ggtttttcta tgcctttaca cagctctgaa
      421 aaatgaaaga ctgacttttg ggcactcaca ccgccggcag gcagttttca gaaaagagga
      481 acaagcacgg tgagcctgcc gccgcagcgt gcgttaataa gtaaaattac atataaattt
      541 tcatataact cagccgatcc gtttcttcag ttcctcctgg gaaataatcc ctttgaccgg
      601 ttcgtaaaaa acagggtcaa tcgggaactt cacttcaacg tcctcatatt ctacaatatc
      661 ttccttcgga tcatagctga taatcgccgg ataggtactg tattttttct caatcatctt
      721 cagaagggcg tagttttctc tgtcgtagga agggacttcc attttgtttg caaactggca
      781 gaaatacttc ggttttccca gccagtagat catcagtgct gttcctcggt cgcatccagg
      841 atctagcaga atgccgtaca gtgcctcata atcgtcatcc cagttatgct cctttgcgag
      901 cttgtaataa ctgacagccg tttggatatc ttcttctttc atagggcagg aataaatctt
      961 ggaaatctcg agtcaaatgc aaataaccgc ttgacccagt tctttttttt gaaaaaaata
     1021 gtcaggggtt ttattatgac aattttgata tccggtactc tgattacact cgctctgctt
     1081 gcactggcaa atttcctgga agtaaagttc aatgcatttc aggatgcaag tctgatgaaa
     1141 aagattctca tctacctcgg aaattctgta ctgtcttccc tggctatggc gatcttaggc
     1201 gcagcactcg gtgcaggggt cggcgacgag agtgcaggtg atgctgtgat tttccttttc
     1261 tttgcaggac ttgggatcgg ttttgttctt ggactgatct tcacaggcgc ctggcagtac
     1321 ttcgccggct gagagacgga atgtatttga aataaattat tttgcaggag gaaaagtttg
     1381 gcataatatg tattcagtat tgatgctcct gataatgtat cttttgggtc tggaaatgaa
     1441 tttacagcct gcagaaactg gatctgattg aatcactgcc ttaagataaa ggtggggaga
     1501 ttctgcttga attttaagta agtctgtcga tatatagaaa aaatgaataa gaagtcctca
     1561 aaggtcaaac agatatggaa agaattctct tttcaaagcg gttttctggc cttcgcggaa
     1621 aattctgaag caaaaaaatc agatctaaaa gacgaagagc gaattcaatt actcaaagca
     1681 aagaatttga aggcagaggc ggtttttttt cgacggtttg aaaatcgctc atctcttccg
     1741 cttgtttatg tttacgattt tacagaaacg gaaatatctg aagaaacaaa aggggagctg
     1801 cataagttta tttggaatag cggagctgtt cctttattca ttatttttca aaaggcaaaa
     1861 atagaaattt ttaactccag caaaaaagta gatttggatg aaaatggaaa tcctagagct
     1921 gtccttcaca aatctcttag cttcaacgaa acaatcgaac tggctgcaat attcagttca
     1981 ttggaaaatg gaacattttg ggatcaaaag gaaaacataa attatttaca atcggactca
     2041 gcatactcca gtttattaaa agagttaaag actgccagaa ccagatttat aaaggaaatt
     2101 aatgtagaag aatcttttgc aaataaactt ttactcattt ccatacttat aaaatacctg
     2161 gaagaaagaa ccggtgcaga caataaaaga attttatatt ttaagaaata taataatgca
     2221 aaaaatttct gtgatgttat cgaaaagaat cagtttctca atttgctgaa tgatttgagt
     2281 actcaaatga atggaaaagt attcgagctt tctgcatcgg agttaaaaat cctgaaagat
     2341 aagaatacca acttgcagcc gctggcagaa tttctttctg caaaattaga cgggaaacaa
     2401 tatgtgcttt ggcctctcta ttccttcaat catctccctg ttgaactgat cagtggaatt
     2461 tatgaagaat ttttacccag agaaaaaagc actgtttata ctccaccgct ccttgttcag
     2521 cttttaattg atcagtccat gcccattcat acaccgagga tggatctgaa aatttttgat
     2581 cctgcctgcg gttcaggaat atttttagta tctgcttttc agagaaaagt ggactggtgg
     2641 aaaatcgaaa attataaaag gacaggtgag tttcaaaatc ctgatttaac agcacttaag
     2701 aaaattctta gagaacgcat cttcggagcg gatgtcaaaa aagaagcagt ccaaattgct
     2761 atctttagtt tgagtattgc gttatgcgat tatctagagc ctactaaaat ctggacggaa
     2821 cttcgctttg atgatttgag tgagaagaat attttagcaa aaaatttctt cacctattta
     2881 aaggaaaacc cagagaacga ttttgattta attatcggta atcctccttt taaaaatttt
     2941 gacaaggaag gagaaagaga cgaagaagct ctgaagattc ataaaaaaga attggaggaa
     3001 aagcaattaa cgtctccaca aaaacagatt gcatttctat tcttaaagta ttccatgcaa
     3061 gttttgaaac ccaatggact tctttgcctg gttatgccat ctgctccttt actttataat
     3121 gatacattag agtatagaaa ttctatttta ttcaacaaat acaatgttcc gcaaatcctg
     3181 gattttacat ttatggacaa atctctgttc cagaaaagaa cttccaaaga agaaaaaaat
     3241 aaagaaagtg aaaaaggaaa aggtgcaatt gttcccacag cagtggtgtt cgtgcataac
     3301 aaggagccag atgaaaaaga tattcttcat attgtgattc gagatacagt cgtagcaaag
     3361 aataaattat actttgaaat tgattcttat gattttcact atgttccgaa aaaaattgcc
     3421 aagactaaag aatggatttg gaaagtaaac cttttgggcg gaggcaggat ttacgatctg
     3481 attgataggt tgagtaaatt gcctactttg ggtgagtata ttaatgaaaa agaaaaaaaa
     3541 tctgagtgga tatttaatga aggatatgaa gtcgggcaaa gaggttctta taaagggaag
     3601 tttattcaaa gaagaaaaca cattatttct gaggcaatta ctgaagatgg aaagattgat
     3661 ataaataaaa ctgagcttga aacgtcagaa tctttctata gggaaagaaa agaggaatta
     3721 tttctgcccc ctttaatgct cttcagacaa gtgattggtg aaaaaagatt acctattgca
     3781 ttggtgaaaa atgtgattcc tgaagtggac gaaaaatatg ttctatttaa ggatggggtt
     3841 atcggtctgc atgttccaca ggaagattct tctagtttag aaattttata taagaagatt
     3901 catgatcgcc attctgttta tcgtttcatt ctaattgcta cttgtggtca gttagcccaa
     3961 agagcgacag aaattaagaa aaaaaatata gagtctttac cttatcccga aaacgacctc
     4021 aaactttcca aattagaaaa aatcattata gaagacgttt taaatttcta ttctaaacta
     4081 ggtggctatg actccactct tgataatcct gtagaagaga aacaattatt cgaatttgga
     4141 aatttactat ctgatatttt aaattcagtg tatgagaaaa ataaaaagaa atttcatcag
     4201 cataaaatca tcattacacc ttcttttgta tgcagttctt ttcagtatgg aagttctgcc
     4261 atttctaaaa aggaaatgat agagaagtcg tcaaagttgg aagaaagtct cgaaatgctg
     4321 gtgcgaaaga acttaggaac cagtgcaagg atcaatcgta tcattctttt ttaccatgaa
     4381 gataaaattt atctgatcaa acctatcaac tgcaggttct ggttaaaatc cattgcgatt
     4441 caagatgcag atgagatttt aatggacttg attgaatcag gatattagaa tgtttgctga
     4501 gtccaaagac gaaaaagcca ggcaagcaga agttaacgga cttgaatcaa atcagcatat
     4561 agattgtatt ttagtttatc tggaaaaata cattcccagt tttactgaaa aaacagaatt
     4621 actggaaaat gaaaaagaga ataaaataaa ttctaaactt tcgatgtatc taaatcgttc
     4681 catagcagag gagaatcact ttccttatat ttttgataga gaaaatcctg attcaaagag
     4741 tcctgaagat attatcgttt cctataaagc aggtcgaaga gaaatcactg tatttgtaat
     4801 cgaatgcaaa agattaccaa cacccccgcc agaagacaga gaaaaagaat atgtttcagg
     4861 taaaggtgca gggatccaaa ggtttaaaga attagatcaa ggtaaaaatt tttcaatagc
     4921 ggctatgatt ggttatatcg aaaaagaaaa tttttcttat tggtttacac aaatcaattc
     4981 ttggatagaa gaactttcca accatgaaaa aacgatatgg aatcacaaag aac
//
LOCUS       DAOQGJ010000043         4998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8752_length_4998_cov_2.667408, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000043 DAOQGJ010000000
VERSION     DAOQGJ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8752_length_4998_cov_2.667408"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PK683_01205"
     CDS             complement(<1..1125)
                     /locus_tag="PK683_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNH07088.1"
                     /translation="MSDFLLSFKKKLSRLGSRMTDGELLPSDATDSIVKIFGISCTLL
                     YIPAVISAENRPMTAFLCLLQPLYNILLNIPYKKYAHIRSRVYLNHWIRFIVSFAHFW
                     IFVLYSKDPDTAWFIVLMQVTGTFFGMRVQLFPVLYGISFWLFFTYSLSYFGLSMQNY
                     TVISFLYLYLIVSTTLISEFFHRRNEKAEAASAVKSAFLANMSHEIRTPMNAVLGFVN
                     ILLQENPREDQIESLKAVQFAGKSLLTVINDILDYSRIESGKTELESANFNLYELVSN
                     VYRTMQIKAEEKKIEFRLDIDKSLPVFVIGDPARITQILLNLIGNALKFTEKGFVSLR
                     IQNLKTEKGLISVLFEVSDSGIGIPEEKQEEIFEIFSQADS"
     gene            1450..2208
                     /locus_tag="PK683_01210"
     CDS             1450..2208
                     /locus_tag="PK683_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH07089.1"
                     /translation="MKKTIILSVFLACTYSVTAQEKPAAEKAKELYAAKDYEKAYSMM
                     EKASPEEKKTAAYWIFLAKINFRLEDTDEGIDNLYSALKIKSDEPEVYLELVKALAST
                     EKNKGAFELCEKALKKFPTSKDLRIQKALLYPKFAKSWEGIKLIESMKEEFPNDSSIL
                     NAEAYIYTILGDLEKAEMSLRWAVSADKSNMVLKRNLGLILEKLGDRKLKEKDKARAK
                     EYYTESVKELNAPASKEKKITDLIEKIQKKADAL"
     gene            2205..3506
                     /gene="mtaB"
                     /locus_tag="PK683_01215"
     CDS             2205..3506
                     /gene="mtaB"
                     /locus_tag="PK683_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002736333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNH07090.1"
                     /translation="MNEKISASEKKIKVAFHTLGCRLNIFETDGLASNLEKLGAVTVT
                     EKDAPDVFIINTCTVTNKADSRNRNVIRSAIKKYPGAKIWVTGCYAETDRQVIENIPG
                     VTGVAGNSEKSALPYMILNDVGLTFDKPEGLNRFSYSDVLPKNHTRAYLKIQDGCSRV
                     CSYCKIPQARGKGESRDWNDVLNQVRFLQDQGIAEIVLTGVNLGWFKDSEGKKSFNRL
                     LSEILDILDYSRLRISSIEPSDVNTELAELISHPRFCKFLHVPLQSGSSSVLRKMKRS
                     YTAETFRKRIESVKKIHPDIFIGTDVIAGFPTESENDFSDTKKMIQDLEIARVHAFPY
                     SPRKGTGAEEFGDVHSKEIKKSRVAELINLSSENLLKYGSKFIGKKLESVLENDGSFL
                     TDNFLKIKLKEYSAHLKTGQFADICITQVSRDKEILFFGTL"
     gene            complement(3672..>4998)
                     /locus_tag="PK683_01220"
     CDS             complement(3672..>4998)
                     /locus_tag="PK683_01220"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07091.1"
                     /translation="LKKNGTFYISYIDMPNAQKSSGQGMSTTLDYIGIFPGNGSLKES
                     LSWNSDLPYFGKETAPIAENYFTEYRKYMHERLVIIAEMLVLISIIILFAFPYFFSLN
                     LTKPLQDLVHAMKTVVSGNLNRSIVPIYNDEIGFLTDSFNKMIDSIRETDAAYRKFVP
                     REFLSYVNKKKITEIRLGDHSIMEMSVLFSDLRSYTSLSEKMTPDQSFKFLNSYLGML
                     GPVIRRNNGFIDKYIGDAIMALFPFRPDDAVRAAVEMQRAIEEYNAERVWFGREPISA
                     GIGIHTGTVLLGTIGEKERMEATVISDAVNLSSRLENLTKLYQAKILISMDTFLELEN
                     QADFSFRILDKVKVKGKEKFVTVVEIMDGYSPEMLEKMMRSRVFFEEGVAFYLSREFE
                     KAVRSFDRVLDELPNDRATLIYMKRAEYFQKNNVPIDWDGVENLEEKFF"
BASE COUNT         1629 a         1031 c          994 g         1344 t
ORIGIN      
        1 agaatctgcc tgagaaaaaa tttcaaaaat ttcttcctgc ttttcttcag gaattccgat
       61 acccgaatcg gatacttcaa aaagaacact gatcagtcct ttttcagtct ttaagttctg
      121 aattcgaaga cttacaaatc ctttttccgt aaacttgaga gcatttccga tcagattcag
      181 aaggatctga gtgatccttg ccggatcacc gataacaaac acagggagag acttgtctat
      241 atccagccgg aattctattt tcttttcctc agcctttatc tgcatcgtgc ggtaaacatt
      301 gctgaccagt tcataaagat tgaaatttgc ggattccagt tcagtctttc ctgattcaat
      361 cctggaataa tccagaatat cgttgatcac tgtcaggaga ctttttccgg caaactgaac
      421 tgccttcagg ctttcaatct gatcctctct cggattttcc tgaagaagta tattcacaaa
      481 acctaatact gcattcattg gagtcctgat ttcatgactc atattggcaa ggaaggcaga
      541 ttttacagca gaagccgctt ccgccttctc attccttctg tgaaaaaatt cagaaatcag
      601 cgtagttgaa acaattaaat aaagatatag aaaggaaatg acggtatagt tttgcatact
      661 cagtccaaaa taggaaagcg aataagtgaa aaaaagccag aaagaaatac cgtacagaac
      721 aggaaaaagc tgaacccgca ttccgaaaaa agttccggta acctgcatca ggacaataaa
      781 ccaggcagta tcaggatctt ttgaatataa tacaaaaatc cagaaatgag cgaaagaaac
      841 aataaatctg atccaatgat tcagataaac cctgctccgt atgtgtgcat actttttata
      901 aggtatgttg agaaggatat tgtagagggg ctgaagaaga cagaggaaag cggtcatcgg
      961 ccggttttcc gcacttatga ctgcaggtat gtacaggagt gtacatgata ttccgaagat
     1021 ttttactatc gaatcagtag catcagaagg gagaagctct ccgtcagtca tcctgcttcc
     1081 cagcctgctg agtttttttt taaaacttaa taaaaaatca gacatttggc aatattttca
     1141 gatccctgca atccatataa tttatatata atctatccat tacttatatg acttgacata
     1201 taataaaaac cgcaatctgc ggttcaataa aaaaaacacc tcggaatatg tctttctgag
     1261 acttgaaatc ttccaaaaat aacagcctga aaacggacag accgcttttt tcagaccgtc
     1321 tgcttccgct gctccagagt ccatccctgc cggttcgcct gcacaaacct gattcctgca
     1381 atgattacgg aacagcagca ttcttcaaat cagttgcgaa aataaaggtc aataaaattc
     1441 tgggcttgta tgaaaaaaac gatcattctt tccgtatttt tagcctgcac atattccgtc
     1501 acagctcagg aaaaaccggc tgcagagaaa gcaaaagaac tgtatgctgc gaaagactac
     1561 gaaaaggcat attctatgat ggaaaaagcc tcaccggaag aaaagaaaac agcagcatac
     1621 tggatttttt tagcaaaaat caattttcgg ttggaagaca ctgatgaagg aatagacaac
     1681 ctttactctg cactcaaaat aaaatctgat gaacctgagg tttatctgga actcgtaaaa
     1741 gccctggcga gcacagaaaa aaacaaggga gcatttgagc tctgcgaaaa ggcactgaaa
     1801 aaatttccga cttccaagga cctgagaata cagaaagccc tcctgtatcc caaatttgca
     1861 aaaagctggg aaggaataaa actcattgaa tcaatgaagg aagagtttcc gaatgattcc
     1921 tccattctga atgcggaagc ttatatttat acaattcttg gagatctgga aaaagcagaa
     1981 atgagtctga gatgggcagt gtctgcagac aaatccaata tggttctgaa aagaaatctt
     2041 ggactgattc tggaaaagct tggagacaga aaactgaagg aaaaagacaa ggccagagca
     2101 aaggaatatt atactgaatc agtgaaagaa ctgaatgcac ctgcatcaaa ggaaaaaaag
     2161 atcacagatc tgattgaaaa aatccagaaa aaagctgatg cgttatgaat gaaaaaattt
     2221 cagcttcgga aaaaaagata aaagtcgcat ttcatacact ggggtgcaga ctgaatattt
     2281 tcgaaactga cggtctggct tccaacctgg aaaaactggg agcagtcact gtaacggaaa
     2341 aagatgcacc tgatgtattc atcatcaata catgcactgt gacgaacaag gctgactcca
     2401 gaaacaggaa tgtaatacgt tccgcaataa agaaataccc tggagcgaaa atctgggtta
     2461 ccgggtgtta tgccgaaaca gacaggcagg tcatagaaaa cataccggga gtgacagggg
     2521 tcgcaggaaa ttctgaaaaa tcagcactcc cttatatgat tctgaatgac gtcggtctga
     2581 cttttgacaa gcccgaaggt ctaaacagat tttcctattc ggatgttctc cctaaaaatc
     2641 atactcgcgc atatcttaag attcaggatg gatgcagcag agtctgctcc tactgtaaaa
     2701 ttcctcaggc aagaggaaaa ggagaaagca gggactggaa tgatgttctg aatcaggtcc
     2761 gatttcttca ggatcaggga attgcagaga ttgttctcac aggagtgaat ctgggctggt
     2821 ttaaagactc tgaagggaaa aaatctttca acagactcct gtctgaaata ctggatatac
     2881 ttgattattc gagacttaga atatcctcaa ttgaaccgtc cgacgtaaat acggaacttg
     2941 cggaactgat ttctcatccc agattctgca aattcctgca tgtgcccctt cagagcggaa
     3001 gttcttctgt tctcaggaaa atgaaaagaa gctacactgc agaaacgttc cgaaaaagaa
     3061 ttgaatcagt aaaaaagatt catcctgata ttttcattgg aaccgatgta attgcaggat
     3121 ttccgactga atcagagaat gatttttctg atacaaaaaa aatgattcag gatctggaaa
     3181 tagccagggt tcatgctttc ccctattctc cgagaaaagg aacaggtgcg gaagagttcg
     3241 gagatgtcca ttccaaggaa atcaaaaaat ccagggttgc agaactgata aatctttcct
     3301 ctgaaaatct tctgaaatac ggaagtaaat ttataggaaa gaaacttgaa tcagttctgg
     3361 aaaatgacgg gtcttttctg acggataatt ttctgaaaat aaagctgaag gagtattcag
     3421 ctcacctaaa gacaggtcag tttgcagaca tatgcattac tcaagtctca agggataaag
     3481 agattctttt tttcggcact ttataatgaa tcgtctttat ggctgaggca gggacactga
     3541 aacagcagca aacctgaatc ctgaccccga acttcgcagc tgcagagaaa gaaagcagga
     3601 aaaaaacaga tttttttgaa actgccctag tgccttcagc cagccgaatt cgaattatat
     3661 tttactgcgg ttcagaaaaa tttttcttcc aggttttcca ctccgtccca gtcgatcggt
     3721 acattatttt tctgaaagta ttcagcacgt ttcatataga tcagagtggc tcggtcattc
     3781 ggaagttcat caagcaccct gtcaaatgaa cgaacagctt tttcaaactc cctgctgaga
     3841 taaaaagcca ctccctcctc aaagaagact ctcgacctca tcatcttttc aagcatttca
     3901 ggagaataac cgtccattat ttcaacgact gtcacaaact tttccttgcc cttcactttc
     3961 accttgtcca gaatccggaa tgaaaaatcc gcctgattct caagctccag aaaagtgtcc
     4021 atggatatca gtatcttcgc ctggtaaagt ttagtcaggt tttccagtct ggaagaaaga
     4081 ttgacagcat ccgaaatgac tgttgcttcc attctttcct tttctccgat cgttcctaaa
     4141 agaactgtac ctgtatgaat tccaatcccg gcgctgatcg gttctcgccc gaaccatacc
     4201 ctttcggcat tgtattcttc aatggctctc tgcatttcca cagctgctct caccgcatca
     4261 tcaggcctga acgggaaaag tgccataatt gcatctccga tatacttgtc gataaagccg
     4321 ttgtttcttc tgatcacagg tccgagcatt ccaagatagg aattcagaaa cttgaaactc
     4381 tgatccggag tcattttttc agacagagag gtatacgacc tgagatcgga aaaaaggacg
     4441 ctcatttcca tgatcgaatg atctccaagc ctgatttcag taatcttttt cttatttaca
     4501 taggaaagaa actctcgggg gacaaatttt ctgtaagcag catctgtctc acggattgag
     4561 tctatcatct tattgaagga atcagtaaga aatcctattt catcattata gataggcaca
     4621 atggatctgt tcaggtttcc ggatactaca gtcttcattg catggacaag gtcctgaaga
     4681 ggtttcgtca ggtttaatga aaaaaaatac ggaaaagcaa ataaaataat gatgctgatc
     4741 agaaccagca tctcagcgat aatgacaagt ctttcatgca tatacttcct gtattctgtg
     4801 aagtaatttt ctgcaattgg agcagtttct ttcccgaaat agggaaggtc ggaattccag
     4861 ctcaatgact ctttcaatga tccgtttccg ggaaaaattc cgatatagtc aagcgtagtg
     4921 gacatcccct gcccggaact tttctgagca ttaggcatat cgatatacga aatataaaaa
     4981 gttccgttct ttttcaga
//
LOCUS       DAOQGJ010000044         4987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8783_length_4987_cov_3.025953, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000044 DAOQGJ010000000
VERSION     DAOQGJ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8783_length_4987_cov_3.025953"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..837)
                     /locus_tag="PK683_01225"
     CDS             complement(136..837)
                     /locus_tag="PK683_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07092.1"
                     /translation="MKKYLLIIAAAICSAASIYAQDSSDQPYSIPAVKNPSVTSPKGY
                     TNFKEEKGDLDGDGIDELLVAYETDKNTDLGKERQIHIFKKEDNKWKLHKKFTGPLLP
                     SQGGGTLGDPFRDIRIKKGVIVIEQSGGSRQQWSYVHKFRKQDNSWELIGATVSYLTE
                     CKEFDKYDYNLNTGKVEAESAELKCDKDFNETGRKNVKKSSFTVKKKDVLMEKFKAGE
                     NEVKIPGKKDVSFFY"
     gene            complement(1370..2086)
                     /locus_tag="PK683_01230"
     CDS             complement(1370..2086)
                     /locus_tag="PK683_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07093.1"
                     /translation="MKKYLLIFAVIFSAGSISSQESSDFPPLIPTVTNPSVPSPKGFR
                     NFKEVKGDLDGDGTDELLVAYEAEKETDSGKERQVHIFKKEDNKWKLLKKFTGLLRLS
                     NEGGMMGDPFQDINIKKGVIIIDQSGGTSSHRWIYVHKFRKQNNSWELIGVTSQDYNM
                     NETSYKYDYNLNTGKVEVEIESTPTATDKNGETVAVEGDPITRKYSFTLKKKTVLMEN
                     FKVGENEVKIPGKKKLSFFY"
     gene            complement(2399..3100)
                     /locus_tag="PK683_01235"
     CDS             complement(2399..3100)
                     /locus_tag="PK683_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07094.1"
                     /translation="MIKYYLIIIFTLIATGPLSSQDSSDEPPRIPTVTKPSVPSPKGF
                     RNIKEAKGDLDGDGKENLLIVYETDRQTDLGKERQIHIFSKDDRKWKLQNKFTGPVLP
                     SEHGGMMGDPFQDIKIKKGVIIIEQSGGSRDKWSYVHRFRKQNNSWLLIGATVDTFTP
                     CEKSDKYDYNLNTGKVEVESSVMKCDENGEEKGKPKTKKSSFTLKKKDVLMENFKAGE
                     NEVKIPSRKDASFFY"
     gene            3569..4462
                     /locus_tag="PK683_01240"
     CDS             3569..4462
                     /locus_tag="PK683_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07095.1"
                     /translation="MHTINIYKYTNIQDASLRLASVKDYEKAERKMNEFERDIQEIPS
                     KERLPYFRLQTEEFSRLIAAPSPDVLVAVCPYCERKIWARIKHGVFTLNASFWVMEYD
                     DCKPVTDDSKCGHLYALDGALNLNENPVPEKIKAFSFIRNKIYMGAEVPFVKPRILKR
                     EGVTAVISSVNIAEKYTGYPVTYFSKEYISQSDFMTGWAREEYCARDEMVSSATFVGE
                     RREPQEYDLTDWISQNKIRWIQEKNNEIVLNSNDDIYPYENLKGRRHPYVIQNGQIKD
                     LPNSEDLQTKTSIETFMPGQG"
     gene            complement(4704..>4987)
                     /locus_tag="PK683_01245"
     CDS             complement(4704..>4987)
                     /locus_tag="PK683_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07096.1"
                     /translation="LIYGGIRADADFILKSANKEHHPHSMAPDWLVLTLSLIDSPFAA
                     ALDTVFLPVTAPRAYLRFRDLEKMNEKLEKQTPAELKKKEEEIIACETL"
BASE COUNT         1378 a          995 c          945 g         1669 t
ORIGIN      
        1 actggcctga tgtatggatg tgtatttttt ctttcccgcc cggagattcg ggaaaaaaag
       61 ccgactgtaa aatcgataca ggtcagattc atctggatta ttttgagtcg gctttctgta
      121 tttctctgct gaagttcagt aaaaaaatga aacgtctttt tttcctggaa ttttcacttc
      181 gttttctcca gctttaaact tttccataag aacatctttt ttcttaacag tgaaagagga
      241 tttttttaca ttttttctgc cagtttcgtt aaaatccttg tcacatttca gctcagcaga
      301 ttctgcttcc acttttcctg tatttagatt ataatcatac ttatcaaatt ccttacattc
      361 tgtcagataa ctgactgttg cacctatcag ttcccaggaa ttgtcctgtt ttctgaattt
      421 gtgaacataa ctccactgct gcctgctccc tccgctctgc tcaatgacta tgacaccttt
      481 tttgatcctg atatcccgaa acggatcccc cagagttccg cctccctgac ttggaagaag
      541 aggaccagta aactttttat gaagtttcca tttattatct tccttcttga atatatgaat
      601 ctgcctttct tttcccaaat cagtattctt gtcggtttcg taggcaacaa gaagctcatc
      661 gattccgtct ccgtcaagat cacccttttc ctccttaaaa tttgtgtacc ctttgggact
      721 tgttacagaa gggtttttta ccgcagggat actgtagggc tggtctgaac tgtcctgtgc
      781 atatatggat gctgcagagc agatcgcagc ggcaatgatt aagagatact ttttcattct
      841 gtttccttga tgatatttcg gatgaccgat aaggctcaca ctcttcagaa aaaagaaatt
      901 ctgtccatca aaaattatat tattaaaatt tatattctaa cattgattat aaaagtctga
      961 acgtttcacg gatttagaga gctgatctca actgtcctct ttttttaaat cgtcgtttat
     1021 ctattgtctt tgctgcggct cctaatgaaa cattgccgag aaagcatcaa tttttgcagg
     1081 atttgacaga aacgagattt gaagtctctc gcgctagctt tactataatg attcttatta
     1141 ttaaaaagat gaatatattc gatgtctctt aatatttcac aggttcaact attaagtttt
     1201 cctgacagga actgtgcaaa agcataaaaa gacgattcac acagaggtca catagatact
     1261 gattttattt gagttgttga aactgatttt tcagcaattc tattgatctg ttctctgtgt
     1321 tctctgtgcg aaaagcagtg cttgtaaaaa acccttgttc gtcaagcgat cagtaaaaaa
     1381 aagacaactt cttttttcct ggaattttca cttcgttttc tcctactttg aaattttcca
     1441 tcaaaactgt ttttttcttc agagtgaaag aatatttcct tgtaatcggg tcgccttcca
     1501 ctgctacagt ttcaccgttt ttatcagttg cagttggggt agattcaatt tcaacttcca
     1561 ctttacctgt attcagatta tagtcatact tataagatgt ctcattcata ttataatctt
     1621 gtgatgttac accgataagt tcccatgaat tgttctgctt tctgaacttg tgaacataaa
     1681 tccatctatg actgcttgtc cctccgctct gatcaataat gatgacacct tttttgatat
     1741 tgatatcctg aaaaggatct cccatcattc ccccctcatt gctgagacga agaagtcctg
     1801 taaacttttt aagcagtttc catttattat cttccttctt gaatatgtga acctgtcttt
     1861 cttttccgga atcagtttct ttttctgctt catacgcaac gagaagttca tcggttccgt
     1921 cgccgtccag atcccctttc acttccttga aattacggaa tcctttcgga cttgggacgg
     1981 aaggatttgt cactgtcggg atcagcggcg ggaaatctga actttcctgc gaggagatag
     2041 atcctgctga gaaaatcaca gcaaaaatta agagatactt tttcattctg tttccttgat
     2101 gatatttcgg atgaccgata aggctcacag tcttcataaa gaagaaattc tgtccatcaa
     2161 aaattatact attcagatct atattttaaa tttacaaaca aatttcgctt acggtgcagt
     2221 gcagcaagtc tgcatttatc ctggagtctt tgcagaatat gtatttagtg tttataataa
     2281 gaatatttat cgtactgatt ctgaatatca ataaactgaa tgtctcgaaa tcttttaata
     2341 tcagattaca ggaaactgtt taagggtgaa tggaaaaatc acccttaaaa cagatggatc
     2401 agtaaaaaaa ggaagcatcc ttcctgctgg gaatcttcac ttcattttct cctgctttga
     2461 agttttccat cagaacatct tttttcttca gagtgaagga ggacttcttt gttttcggtt
     2521 tgcctttctc ttcaccgttc tcatcacatt tcattacaga agactccact tccacttttc
     2581 ctgtattcag attataatcg tacttgtcag atttttcgca gggagtgaat gtatcgaccg
     2641 ttgcaccgat gagcagccag gaattgttct gttttctgaa tctgtgaaca taactccatt
     2701 tgtcacggct tcctccgctc tgctcaatga tgatgacgcc ttttttgatt ttgatgtcct
     2761 gaaaaggatc tcccatcata ccgccgtgtt cactgggaag aacagggcct gtaaatttat
     2821 tctggagttt ccatttcctg tcgtccttgc tgaatatatg aatctgtctt tcttttccta
     2881 aatcagtctg tctgtcagtt tcatatacaa tcagtaaatt ttcttttccg tccccgtcca
     2941 gatctccttt cgcttcctta atgttcctga atccttttgg gcttggaaca gaaggcttcg
     3001 ttaccgtagg aatgcgtggc ggttcatctg agctgtcctg tgaagacagg gggcctgttg
     3061 caattaatgt aaagataatg attaaataat attttatcat tctgcgtcct ttaatgtcgt
     3121 ttcggaagac cgataaactc aatttttttc aaaatcagaa ttaagtctat ataaaaatca
     3181 ggatttctga tctcgcaggc tatggagccg ctgctgcgct ttgcctttcg ggcattggga
     3241 attcgggaaa aaattatccc cttttttcct tagggaatcg tttttttgta aaacctttac
     3301 tcgaatctca tctttttgga agggaaaggg cagggaaggt ggatttgaca gtctgcacag
     3361 aataccgtta tgaatttggg tgaacctgac attagcaagg tacaaaaaac agtcagctgg
     3421 agcagcagaa atctgataaa agtctctaga ctttttttga attcgaaata atctgtataa
     3481 agaagagatt aaacctgtga gaaatgaaat atttcaggct gccataaaga ctggctttgt
     3541 ctgcttaatc tgtttcattg aattctgaat gcatacaata aatatatata aatatacaaa
     3601 tattcaggat gcaagcctga gacttgcttc tgtcaaagat tatgaaaaag cggaaagaaa
     3661 aatgaatgag tttgaaaggg atatccagga aatcccttca aaggaaagac ttccttattt
     3721 ccggcttcag acagaggaat tcagcaggct gatcgcagca ccgagtccgg atgttctggt
     3781 tgcagtctgt ccatactgtg aaagaaagat ctgggcaaga atcaaacatg gggtattcac
     3841 actgaatgca agtttctggg taatggagta tgatgactgt aaacctgtca ctgatgattc
     3901 aaaatgcggg catctgtatg cactggacgg tgcattgaat ctgaatgaaa accctgttcc
     3961 tgaaaaaatc aaagcattca gctttatcag gaataaaatc tatatgggtg ctgaagtccc
     4021 gttcgtcaaa cccagaattc tgaaaaggga aggagttact gctgtcatca gctctgtcaa
     4081 tattgcagaa aagtatacag gatatcccgt cacatatttt tcgaaggaat atatttctca
     4141 aagcgatttt atgacaggat gggcaagaga ggaatactgt gcacgggatg aaatggtttc
     4201 ttctgccaca tttgtcggtg aaagacggga gcctcaggaa tatgatctta cagactggat
     4261 cagtcagaac aagatcagat ggattcagga aaaaaataat gaaattgtat taaattcaaa
     4321 tgatgatata tatccatacg aaaatcttaa gggaagaaga catccgtatg taatacagaa
     4381 tgggcagata aaagatctgc caaattcaga ggacctgcag acgaagacat ccattgaaac
     4441 ttttatgccg ggacagggat gatcttcttc ctcagaatcg tcttgcgtga gtcttctttc
     4501 ttctgtatct ccggtcagtt ttctttgata taatctcggt tttaatataa gcaaggtcct
     4561 tgagcatctc aagagcctat gcagccttct tctgattttt tatgcttatt gctattttca
     4621 ttttatgact ccttttctca gtttaagcag tctctttgct gagtcaatat gtattctttc
     4681 tctccttatt gatcctcagc agtttaaaga gtctcgcagg caatgatctc ttcttctttt
     4741 ttcttcagtt cagccggagt ctgtttttcc agtttctcat tcattttttc aagatctctg
     4801 aatctcagat aggctctggg agcagtgacc ggaagaaaaa cagtatccag cgctgctgca
     4861 aacggagaat caatcagtga cagagtcaga acaagccagt caggggccat actgtgcgga
     4921 tggtgttcct tgtttgccga cttgaggata aaatcggcgt cagcacggat tcctccgtaa
     4981 atcaggg
//
LOCUS       DAOQGJ010000045         4981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8797_length_4981_cov_3.447219, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000045 DAOQGJ010000000
VERSION     DAOQGJ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8797_length_4981_cov_3.447219"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PK683_01250"
     CDS             <1..286
                     /locus_tag="PK683_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07097.1"
                     /translation="YLNRFQLMLKLKEFLEIHGKTKITDVNTGVLKMYILHSKNQNKT
                     PKEKLINYKLLRVDERLLNGGTEEISERDSIIVEFLIEELMKYYSRSRKK"
     gene            408..1142
                     /locus_tag="PK683_01255"
     CDS             408..1142
                     /locus_tag="PK683_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07098.1"
                     /translation="MDIELYYGKSGKNDYNTIIDMFPKNSINSVRTSSLPLLAFWKET
                     NSRLKELLNSLQIENKEKFSLCFEYPTSSGGNGKASMTDLMIFSNSKKLAIEAKFTEI
                     GEQYELIRNWIVKGNKENRKEVLTKWIDIVKPFSQTELNIERMESIPYQFLHRTASAC
                     DKFTYAFVIYQIFFDSETEKFLQRFKEKLKQAKEIINPNQKLKFYVWPIEVKQIIEES
                     EKENAFEKMKTENIYEFIKVNNPEQI"
     gene            1448..2140
                     /locus_tag="PK683_01260"
     CDS             1448..2140
                     /locus_tag="PK683_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07099.1"
                     /translation="MDTKKRDLINFILVLIGLAAIIASLLEKELNAANILDAVRNIAT
                     MLVTIAVYLISLYNIEQNISREDPFLISGTDAMEKIKKKYSHILIGPCYSRENYDPKK
                     GKGQKYLFIKEKNKKLKAQIMSLQLLQMKLINLQISTTMMNVLGIVEDRAALLSQVQE
                     TVMNKISKKYQIPAESIEIGEAKNDKNRCLEIDCNDFEIDHKKFGNLVFDVLDIAIAS
                     LIEKSENAKIKA"
     gene            complement(2246..3298)
                     /locus_tag="PK683_01265"
     CDS             complement(2246..3298)
                     /locus_tag="PK683_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HNH07100.1"
                     /translation="MIFYKFIIYIILLFTVHCGKNEVKPPKVRYFPENFYLYNRSSPL
                     RAEILPGIGNTVFQIYSHGRTVLFSPVPREDLLKTEQLIGIPLMFPWANRLEGNYFYH
                     NDKKYILNDNKNTLRRDKNGLPIHGLILKDKNWKPEIVRNSETGSYYDSSFSFGKEFQ
                     NLFPFEADLRQEIELIDNKLIFTLTVKNGSSDSFPLSLGYHPYFLVSEEDRKTVSLFT
                     NASSYYETDSKLIPTGKKKKSSSVLPKDGKLEKKTLDTVFTDFPKGALRYSVLNFKKR
                     NIRLELIEGFDHLVLFVPEGKDFICMEPMLGPTNVFNLAHRKKYKGLQKLDPGRSFRA
                     SFSIQITEHPEEIHRK"
     gene            complement(3295..3765)
                     /locus_tag="PK683_01270"
     CDS             complement(3295..3765)
                     /locus_tag="PK683_01270"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNH07101.1"
                     /translation="MSLNPKEFKVYDTDFRERVIADFKRQKVMSTIGASLLRVDAGEV
                     EILLPFSESLTQQNGFLHAGIVTAIVDSACGYAAYSLMPKNASVLSIEFKINLCSPSI
                     GEKFIAVGKVRKAGKTVTVTEGEVWAFQSGEKKLTALMQGTMMTVFSLNEQKVQ"
     gene            complement(3766..4137)
                     /locus_tag="PK683_01275"
     CDS             complement(3766..4137)
                     /locus_tag="PK683_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNH07102.1"
                     /translation="MNLFYKKNIILLLILLFNCYPEYRVPYLSGIIDVKHTSRGENNF
                     FYPSRNDQITNVVGSGKLLKQGKSCSISVFFVEPYVFVKGGSIRSAAASADISKIGSV
                     EYSLFSFFGLYINECIIVWGE"
     gene            complement(4162..4497)
                     /locus_tag="PK683_01280"
     CDS             complement(4162..4497)
                     /locus_tag="PK683_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HNH07103.1"
                     /translation="MKIKKIRLLFTLLLFLILTECGSIGETYRPAPGFLYSNVKFDGD
                     FNPENNVPESKTAEGCIQHFLRIYSWGDSGAGSIANRAGIKKISFIDHKYTEFLLIYG
                     KYCTVVYGE"
     gene            complement(4499..4783)
                     /locus_tag="PK683_01285"
     CDS             complement(4499..4783)
                     /locus_tag="PK683_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNH07104.1"
                     /translation="MKKIIIFIILFIFTDCRGIIYRQEKIPGKLGNAKAIKEASVCMF
                     NYLFIVSSGDASIKAAKEKGSISKISSVEIEVTSYFYYLVRKHCLILSGE"
     gene            complement(4788..>4981)
                     /locus_tag="PK683_01290"
     CDS             complement(4788..>4981)
                     /locus_tag="PK683_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07105.1"
                     /translation="GFGAGAGAGMNIFLTPEFGFQVDISGRKKWLRSEHLGNSNLTTA
                     QIQVGVVFNFENIAKFTEK"
BASE COUNT         1645 a          867 c          941 g         1528 t
ORIGIN      
        1 gtacttgaac agatttcagc tgatgctcaa gctgaaggaa tttctggaga ttcacggaaa
       61 aacaaaaatt acagatgtga atacgggtgt tctgaaaatg tatattcttc attcgaaaaa
      121 tcagaacaaa acccccaaag aaaaactgat caattataaa ctgctccgag tggacgaaag
      181 acttttgaat gggggtaccg aagaaatcag tgaaagggat tccatcatcg ttgaatttct
      241 gatcgaagag ctgatgaagt attactccag aagcaggaag aagtgagagt tttccgggtt
      301 atggtttctt tagctttgat tcattttata aatcaagtgt tagaagagat taaaaaagca
      361 agtatggtat gttacagcta attatagatt aaggagattt aagggatatg gatatcgaat
      421 tatattatgg gaaatcagga aaaaacgatt acaatacaat aattgatatg tttcctaaaa
      481 atagtatcaa tagcgttcgc acttccagct tgccactatt agcattttgg aaggagacaa
      541 atagtcgact gaaagaatta ttaaattctt tacagattga aaacaaagaa aaattctcat
      601 tatgctttga gtatccaact tccagtggcg gaaatggaaa agcttcaatg acagatttaa
      661 tgatcttttc gaattctaaa aaattagcaa ttgaagcgaa gttcactgaa ataggagaac
      721 aatatgaact cataaggaat tggattgtga aaggaaataa ggaaaatcga aaagaagttt
      781 taactaaatg gatcgacata gtcaaaccat tttcccagac agagttaaat attgaaagaa
      841 tggaaagtat tccgtatcaa tttcttcatc gtactgcctc agcatgtgac aaattcactt
      901 atgcttttgt tatttatcaa atattttttg actccgaaac agaaaaattc ttacaacgct
      961 ttaaagaaaa attgaagcaa gcaaaagaaa taatcaatcc caatcaaaaa ttaaaatttt
     1021 atgtatggcc gattgaagtt aagcaaatta tcgaagaaag tgaaaaagaa aatgcattcg
     1081 aaaaaatgaa aacagaaaac atatatgagt ttataaaagt gaataatccg gagcaaattt
     1141 gattcaaaaa aagtattttc tagaataaat ttaagagggc tgaaaatggt aaacagtggt
     1201 ataagaaaat caattgttct gctggttcta atgttctgca aatcgtatgg caataaattg
     1261 aagggaactt gtaaatgtga tggagggact gttgaaggac ttgaattctt aactggagat
     1321 caggtgattc tatctttaag gttgctggaa cgaactgaaa ggtgaaacat attttggcat
     1381 tatggagctc ggcagctgca ggaagacatc aaactgatgc gaaatattca gtcaaaggaa
     1441 aacggaaatg gatactaaaa aaagagacct gataaatttc atcctagtat tgataggact
     1501 tgcggctata atagcgagcc tactggaaaa ggaattgaat gcagcgaata ttctcgatgc
     1561 agtcaggaat atagccacaa tgcttgtgac gattgcggta tatcttatat ccctttataa
     1621 tattgaacag aacatcagca gagaagatcc atttttgata tctgggactg atgcaatgga
     1681 gaaaataaag aaaaagtatt ctcatatcct gattggtccc tgttattccc gtgaaaacta
     1741 tgatcctaaa aaagggaaag gacagaaata cctgtttata aaagaaaaga ataaaaagct
     1801 gaaagctcag ataatgtctt tgcagcttct gcaaatgaaa ctgataaatt tgcaaatttc
     1861 cactacaatg atgaatgtcc tgggaatcgt tgaagatcgt gcagccctgc tttcacaagt
     1921 tcaggaaaca gtgatgaata aaatttcaaa gaagtatcag atccctgctg aaagcattga
     1981 aattggagaa gccaagaatg acaaaaaccg ctgtctggaa attgactgta atgattttga
     2041 aatagatcat aagaaatttg gcaatcttgt ctttgatgta ttggacattg caattgcttc
     2101 tctcattgaa aagagtgaga atgcaaaaat taaagcatag gccttttttc atatatgagg
     2161 aaggtatcaa aagattatta gagttgctga atggacgccg cggcacataa agctgatccc
     2221 tgcaagccta ttgatggaat cggtttcact tcctgtgaat ttcttccgga tgctcagtaa
     2281 tctgaatgct gaaagatgct ctgaagcttc tgccagggtc gagtttttga agtcccttgt
     2341 atttttttct gtgggcaaga ttgaaaacat ttgtcggccc cagcatcggt tccatacaga
     2401 taaagtcctt tccttccgga acaaaaagaa ccagatgatc gaatccttca atgagttcga
     2461 gacgaatatt tctttttttg aaattcaaca cagaatatct gagagctcct ttaggaaagt
     2521 ctgtaaacac tgtatccaga gttttctttt ccagttttcc gtctttagga agcactgagg
     2581 atgatttctt tttctttccc gtaggaataa gttttgaatc tgtctcataa taactggatg
     2641 catttgtaaa aagacttact gtttttctgt cttcttcgct gacaagaaaa tacggatgat
     2701 accctaaaga gagaggaaaa gaatcggaag aaccattctt tacagtaaga gtgaatatca
     2761 gcttattgtc gatcagctcg atttcctggc gcaggtctgc ttcgaatgga aacaaattct
     2821 gaaattcctt tccaaagctg aaactggaat cataatagga gcctgtctct gaatttctga
     2881 cgatctcagg tttccagttt ttatccttca aaatcaagcc atgaatagga agcccgtttt
     2941 tgtctcttcg gagtgtattt ttattatcat taagaatgta ttttttgtca ttatgataaa
     3001 aataattgcc ctccagtctg tttgcccagg gaaacataag aggtatcccg ataagctgtt
     3061 cagtcttcag aagatcttcc cttggaacgg gagaaaagag aacagtgcgt ccgtgagaat
     3121 aaatttgaaa gacagtattc cctatacccg gaagaatttc agcgcgaaga ggggatgatc
     3181 tgttataaag ataaaagttt tccggaaaat acctgacctt tggaggtttt acttcattct
     3241 ttccgcaatg aactgtaaat aataaaatta tatatattat aaatttataa aaaatcattg
     3301 caccttttgc tcattcaggc tgaacactgt catcattgtt ccctgcatca gtgctgtcag
     3361 tttcttctct ccactctgaa atgcccagac ttctccctct gttactgtga cagtctttcc
     3421 tgcttttctg acttttccga cggcaatgaa tttctctccg atgctcggtg aacagagatt
     3481 gattttgaat tctatagaga gtactgaagc atttttaggc atcaatgaat atgctgcata
     3541 tccgcaggca ctgtccacaa tagcagttac tattccagcg tgaagaaaac cgttctgctg
     3601 agtcagactc tcggaaaaag gaagaaggat ttccacttca cctgcatcta ctctgagaag
     3661 agaagcaccg attgtggaca ttactttctg tcttttaaaa tcggcaatga ctctttccct
     3721 gaagtcagta tcatatactt tgaattcttt tggatttaag ctcacttatt ctccccatac
     3781 aataatgcat tcgtttatgt aaaggccgaa gaagctgaaa agagagtatt ccactgaacc
     3841 gattttactg atgtctgcag aagctgccgc agatcttatg cttcctccct tgacgaagac
     3901 atacggttca acaaaaaaaa ccgatattga acaggacttc ccctgtttca acagttttcc
     3961 cgatccgaca acatttgtta tctgatcatt tctggaagga tagaagaaat tattttctcc
     4021 tctggatgta tgctttacat caatgattcc tgaaaggtac ggaactctgt actccggata
     4081 acagttgaat aaaagtataa gtaataatat aatatttttt ttatagaata aattcatatc
     4141 ctcttccgtc taactgtgtc attattctcc gtatactact gtgcagtatt ttccgtatat
     4201 cagaaggaat tccgtatatt tatgatcaat gaaagagatt tttttaatcc cggctctatt
     4261 tgcaatggat cctgcgccag agtcacccca ggaataaatt cgtagaaaat gctgaatgca
     4321 cccttctgct gtttttgatt ctgggacatt gttttcagga ttaaaatcac cgtcaaactt
     4381 cacattgctg tataggaatc cgggggcagg tctgtatgtt tctccaattg atccgcattc
     4441 tgtcagaata aggaataaaa gaagcgtaaa taagagtctt atttttttga tcttcaattt
     4501 attctccaga aagtataaga cagtgcttac gcactaaata atagaaatag gatgtaactt
     4561 cgatttcgac tgaactgatc tttgaaatgc tgcctttttc ttttgctgcc ttaatgcttg
     4621 catctcctga tgacacaata aataaataat tgaacataca gactgaagct tcttttattg
     4681 cttttgcgtt tccaagtttt ccgggaattt tttcctgcct gtaaataatt cctctgcagt
     4741 ctgtaaaaat gaacagaata ataaatatta ttattttctt caatttatta tttttccgta
     4801 aactttgcta tgttctcaaa attgaaaaca acacccactt gaatctgagc tgtcgtaaga
     4861 tttgagtttc caagatgttc tgatctaagc catttttttc tgccagaaat gtctacctga
     4921 aatccaaatt caggagtcag gaaaatattc atccccgcac ctgctcccgc accaaagcct
     4981 g
//
LOCUS       DAOQGJ010000046         4973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8824_length_4973_cov_2.900366, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000046 DAOQGJ010000000
VERSION     DAOQGJ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8824_length_4973_cov_2.900366"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PK683_01295"
     CDS             complement(<1..576)
                     /locus_tag="PK683_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNH07106.1"
                     /translation="MPAKKSVKKISNTKKKTSVSKKKKKELSVTSELETVQPNEISEV
                     EEFDPREKYMSIWEHLEEFRIVLLKSLSFLAVCMGVTLFFSSDLYKVMVVPYKNVLGA
                     DAKFFQIKLMAPLVIYLKMSFLISLLITLPVILYLVWGFISPAVSEATEKYGRLIIGF
                     STFLFWAGIFVCWFTVFENLLKVFLVYYNPPD"
     gene            complement(577..813)
                     /locus_tag="PK683_01300"
     CDS             complement(577..813)
                     /locus_tag="PK683_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNH07107.1"
                     /translation="MPPLAFLNLGPWEIALIVFLVLIFFGGKRLPSLAKDLGNGIKEF
                     RKSLSAAAEDDSDRREIPDGKKTPEAKGKKTRSS"
     gene            961..1527
                     /locus_tag="PK683_01305"
     CDS             961..1527
                     /locus_tag="PK683_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07108.1"
                     /translation="MRKKVLIALLAVFALTLANCSSEPKKETPAETADGKCTSGDCKN
                     GRGTVTYNDGSKYEGDFKDGKMNGEGALTFANGDSYKGNFANDMFSGKGTYKYANGDI
                     YEGDFKNDLFDGNGVLTTKKGNKYTGGFKEGKFDGDGTVTQPDGDTLSGTFKMDIPVK
                     GTVKSKDGRVNVQKDFTQSMPAELKRRL"
     gene            1577..2635
                     /locus_tag="PK683_01310"
     CDS             1577..2635
                     /locus_tag="PK683_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07109.1"
                     /translation="MENQSIFKSGWIVSRNYDLFFFIGSFLFALMFYGIYRLCIYSGL
                     EVNGLSILVTYFIFTAVFDHPHIFQTFSRTHADGEEFARRPKTYTLGILAFIGAGFIL
                     TGLGYVKELIIFAAYFGSYHVIKQHYGFMRIYKARNGDVLQTDNIIDSLTFYSGMFAC
                     FFNNFTYDGKPVLIYGDLNVVFPGTPDFVGETVWMIFKGSAAVFVLRQIQLVISGKQI
                     NLPKILLMSSALFIHYFVFFATETAFLVAESIETAFHAVQYQGWMMHFQEKRYPNARK
                     IALKWFLVSLLYGVTAGVIEIYGLLSYVWAVWLFVPFSMLILFHYFIDGYIWKFRDYP
                     ELGKLLLSGSSPSAESVS"
     gene            2713..4065
                     /locus_tag="PK683_01315"
     CDS             2713..4065
                     /locus_tag="PK683_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07110.1"
                     /translation="MNKKHNLVILSLIVFQCSLLNRKAAPPPVIVPPKPAAVEIKKPV
                     AMKEPAVPAKKEPAKDYRKELRAIKIEDKNRFFDCEPIFSSTKPVEMKFTYPFFDYFA
                     EEKKNYIFEHFLAEYETSPFVISYANFEKRFQGRPVILGFRGDGAGFHRTDITGGLQK
                     LAAKLPTSLFMTIDEYRYAEMNDKNLDSSKVMPLCHKRKYDEGFIGPEIPAFEKGSIW
                     KFLDNLIPKEDLIVFSYSNGTIPRNKLFERNYKPGYYPDYKNVSDPEAFLKEYIAKTS
                     APDKNAKRIKGIVDVEGNYMSTKPAWDLLAFLKTHIEPDPLAFFYTAERIGKEDGYPV
                     HVLMIQALGLMGEEDENGVITYTNLNKNIMIEIVTNPEEPYYSGGGLDLQQATKFEYY
                     LKGKTERLPQLNHGNIPTWVIDRLNTKYKDMFPALFKPARGFKPLYQIPTKNSSKKRY
                     "
     gene            complement(4049..>4973)
                     /locus_tag="PK683_01320"
     CDS             complement(4049..>4973)
                     /locus_tag="PK683_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH07111.1"
                     /translation="YSSVCLAVLSGAVKPENLLNPYAAVPSWKSFFFSSVSLSVIQFL
                     LYKSVSSIAMENKRLLFFAESERKKAEEARAEVEVLAESRKRLSMIGQTVSGIVHDIK
                     NPISSIKAFAELMNDESLSEEKKDYLQSIEREAERLGSMTYEILDFSRGSFTLNLKKV
                     SPQAFLEEIRKFIIPDFDFMNIRLYIICETDELMNIDSERLRRVFINMANNAREAFSS
                     LRRECYLKIRAEKENGYFLFTVEDNGRGIPAEVKSRLFDAYSTYGKSRGTGLGLYMSK
                     LIIEKHQGTVECFSEEGVGTRFIIRIPNIFS"
BASE COUNT         1522 a         1033 c         1027 g         1391 t
ORIGIN      
        1 atcaggagga ttgtaataca ccaggaagac tttcagcagg ttttcaaaaa cagtgaacca
       61 gcatacaaag attcctgccc agaaaagaaa agtggaaaag ccgatgatga gccttccgta
      121 tttctctgtg gcttctgaaa cagcaggcga aatgaatccc cagacaagat agagaatgac
      181 tggaagagta atcaggagcg atatcagaaa cgacatcttg agataaatta caagaggcgc
      241 catcagcttg atctgaaaga attttgcatc tgccccaaga acattcttgt acggaacgac
      301 catgaccttg tataaatcgg atgagaaaaa aagagtcact cccatgcaga cagcaagaaa
      361 gctcagactt ttgaggagaa caatcctgaa ttcctccagg tgttcccata tgctcatata
      421 tttttcacgg ggatcaaatt cttcaacttc tgaaatttca ttcggctgca ctgtttccag
      481 ttcagaagtt acagacagct cttttttttt ctttttggaa acagaagtct tcttttttgt
      541 attactgatc ttttttactg atttttttgc gggcattcag ctggatctag ttttttttcc
      601 ctttgcttcg ggagtttttt tcccatcagg tatttctctt ctgtcggaat catcctccgc
      661 tgctgcagaa agagattttc tgaattcctt gataccgttt ccgagatcct ttgcgagaga
      721 tggcagtctt ttgcccccga aaaaaatcag cacaagaaaa acaatgagtg cgatttccca
      781 aggacctaaa tttagaaatg ctagaggcgg catatagaag aatctccttt gattaagttt
      841 cagattcatg cactggaggt caagcagatt tgagaaatta tctgatcttt cacttgacat
      901 gagacttgcg taaattacgg ttttataaat cagagactta ttgaatttaa ggagatccga
      961 atgaggaaaa aagttttaat tgcactatta gcagttttcg cactgacctt ggcgaactgt
     1021 tcttccgagc cgaaaaaaga gacaccggca gaaactgccg atggaaaatg cacttccggg
     1081 gactgtaaaa acggcagggg aactgtgact tacaatgacg gttccaaata tgagggagat
     1141 ttcaaagacg gaaaaatgaa cggagaagga gctcttacat ttgccaacgg tgattcttac
     1201 aaggggaatt ttgcaaatga tatgttctcc ggaaaaggca cttataaata tgcgaacggg
     1261 gacatttatg aaggagattt caaaaacgat ctttttgacg gaaacggtgt tctgactaca
     1321 aagaaaggaa ataaatacac cggaggattc aaagaaggaa aattcgacgg agacggaacg
     1381 gtcactcagc cggatggaga cacactttca ggaacattta aaatggatat tcctgtgaaa
     1441 ggaacagtga aatcaaaaga cggaagagtc aatgttcaga aagatttcac tcagagtatg
     1501 cccgctgaac tgaaaagaag actgtaatat agaaactgcc cgatgcagct gaacctgccg
     1561 ggcagtgaag cagacaatgg agaatcagag catattcaag tcaggatgga tcgtaagcag
     1621 aaattatgat ctattttttt tcatagggag ctttctgttt gcccttatgt tttacggaat
     1681 atacagactc tgcatttatt caggtctgga agtgaacggt ctttcaatac tcgtcacata
     1741 cttcattttc acggcagtct tcgatcaccc ccatattttt cagacatttt cgagaaccca
     1801 cgcagacggt gaggaatttg ccagaagacc gaaaacttac actctgggga ttcttgcatt
     1861 cattggagca gggttcattc tgaccggtct cggatatgtg aaggaactga ttatctttgc
     1921 agcatatttc ggttcatatc atgtaataaa acagcattac ggattcatga gaatttacaa
     1981 ggccagaaac ggtgacgttc ttcagacaga caatatcatc gacagtctga ccttctattc
     2041 aggaatgttt gcatgctttt tcaacaattt cacttatgac ggaaaacctg ttctgattta
     2101 cggagatctg aatgtcgttt ttcccggcac tcctgatttt gtaggagaaa cagtctggat
     2161 gatcttcaag ggttctgctg cagtctttgt actgagacag atccagctgg tcatttccgg
     2221 aaagcagatc aatctgccaa agattctcct gatgagttct gcattattta ttcattattt
     2281 tgtatttttc gcaacagaga ctgccttcct cgtggcagaa tcaatagaga ctgcttttca
     2341 tgcggttcag tatcagggct ggatgatgca ttttcaggaa aaaagatatc caaatgcccg
     2401 aaaaattgca ctgaaatggt ttttggtctc tctgctttac ggagtgactg caggggtcat
     2461 tgaaatatac ggacttctct cctatgtatg ggccgtctgg ctttttgtcc ccttttccat
     2521 gcttatcctg ttccattatt tcatagacgg ttatatatgg aaattcagag actatcctga
     2581 actcggaaaa ctgcttctct caggcagcag tccttcagct gaatcagttt cgtaaaactt
     2641 gacagccgag cttgcttaaa aaaatctgta actgtaaaaa ttttccgaca gatcttcccg
     2701 aaagaatacc caatgaataa aaaacacaat cttgtaatcc tttccctaat cgtattccag
     2761 tgttcacttc tgaacaggaa agccgcccct cctccggtca ttgttcctcc gaaaccggct
     2821 gctgtggaaa ttaagaagcc ggttgcaatg aaagaaccgg cagttcccgc taaaaaagaa
     2881 ccggccaaag actacagaaa agaactgaga gccataaaaa tcgaagataa aaacagattc
     2941 tttgactgtg agccgatttt cagttctact aagcctgtag aaatgaaatt tacctatccg
     3001 ttctttgact actttgcaga agaaaaaaag aattatatct ttgagcattt tcttgccgag
     3061 tatgagacat caccttttgt catatcgtac gccaactttg aaaaacgttt ccaaggcaga
     3121 cctgtgatac tgggattcag aggagacggt gcaggctttc acagaacaga tatcaccgga
     3181 ggacttcaga aacttgcagc caaacttccc actagtctgt ttatgaccat agatgaatac
     3241 agatacgctg aaatgaatga caagaatctg gattcatcca aggtaatgcc cctctgtcat
     3301 aaaagaaaat atgatgaggg tttcatcggt ccggaaatcc ctgcatttga aaaaggaagt
     3361 atttggaaat ttctggacaa tctgattcct aaggaagatc tgattgtttt ttcttacagc
     3421 aacggaacaa ttccgagaaa caagctcttt gaaagaaatt ataagccggg ctattatccg
     3481 gattacaaaa atgtatccga tcctgaagca tttctgaaag agtacatcgc caaaacttcc
     3541 gctccggaca aaaatgcaaa acgcatcaag ggcatagtcg acgtggaagg aaactatatg
     3601 tccacaaaac cggcctggga ccttctggca tttctcaaga cgcatatcga acctgatccg
     3661 ctggcattct tttacaccgc agagagaatc ggcaaggaag acgggtatcc tgtgcatgta
     3721 ctgatgatac aggcattggg acttatgggt gaagaagacg aaaacggtgt catcacttac
     3781 accaacctga acaaaaacat tatgatagaa atagtgacga atcctgagga accttactat
     3841 tccggaggag gtctggatct tcagcaggcc acaaaatttg aatactatct gaaaggaaaa
     3901 acggaaagac ttcctcagct gaatcatgga aacattccta cctgggtcat agacaggctg
     3961 aatacaaaat acaaggatat gtttcctgct cttttcaaac ctgcgagagg attcaagcct
     4021 ttataccaga tcccgacaaa aaacagttct aagaaaagat attaggaatt ctgataataa
     4081 aacgggttcc taccccctct tctgaaaagc attctacagt tccctgatgt ttttcaataa
     4141 tgagcttgga catataaagt ccaagccctg ttccccttga cttgccataa gttgaatatg
     4201 catcgaacag ccttgacttt acttcggcag gaatgccgcg tccattgtct tcaacagtaa
     4261 aaagaaaata cccgttttcc ttttcagccc tgatcttaag ataacactct cttcgcagtg
     4321 atgaaaaggc ttctctggca ttatttgcca tattgataaa aactcttctg agcctttcgc
     4381 tgtcgatatt cattaattca tctgtttcac atattatgta taagcgtata ttcatgaaat
     4441 caaaatcggg aattatgaat tttctgattt cttccaggaa ggcctgaggc gaaacctttt
     4501 tcagattcag agtgaagctg cctctgctga aatccagaat ttcatatgtc attgaaccta
     4561 agcgttcagc ctctctttct atgctctgaa ggtagtcctt tttttcttca ctgagactct
     4621 catcattcat cagttcagca aatgccttga tggaggaaat aggattcttg atgtcatgca
     4681 cgatgcctga aaccgtctgc cctatcatag acagtctttt gcgggactcg gccagaacct
     4741 ccacctccgc tctggcctct tctgcctttt ttctttcaga ttcagcaaaa aagagaagtc
     4801 ttttgttttc cattgcaatg ctcgagactg atttgtacag aagaaactga atcacggaaa
     4861 gagagactga actgaaaaaa aaacttttcc aggatggaac tgctgcatac ggattcagaa
     4921 ggttttcagg ctttacagca ccggaaagaa cagcgaggca tactgaactg tat
//
LOCUS       DAOQGJ010000047         4931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8933_length_4931_cov_2.820139, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000047 DAOQGJ010000000
VERSION     DAOQGJ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8933_length_4931_cov_2.820139"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PK683_01325"
     CDS             complement(<1..803)
                     /locus_tag="PK683_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNH07112.1"
                     /translation="MGRVFKNPEELLKKFVRKKDHIHLSAVMSRPNLLINQLCRVFYH
                     QKPEFTVSVTGIHSNANAIALSGICSRMITGFLGDNYPRPIPNRLYENVWKGNPFELE
                     LWSLWSFVQRLMAGSMKLEYTVSNSLLGSDMISDKLGKTAFVQNDSEGKEILLLKAMN
                     PDITLIHSVVADEEGNFVLSEPGGESFYGALAAKRGILVSAEKIVPAGMIPSSLISVS
                     GTNVLGVVEIPFGTHPQSLRVPDQPFFASLGLETYLDDYSFIEETNRLTG"
     gene            complement(803..2071)
                     /locus_tag="PK683_01330"
     CDS             complement(803..2071)
                     /locus_tag="PK683_01330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNH07113.1"
                     /translation="MAKPRVVITGIGTVLPGALTVDDFWKNISEGQSQIDFITRFPTD
                     DLSVKVAAELKGFDPKINLPPRLDPKFVDKYSYETLVLMNTLGKCLEDSRLDYEGWEP
                     ERVQLFDSSSRSSMYWWDEAYRIHYTEPEMDSRFNRHAILTGMASNPATLSALAYNVQ
                     GFVTCISGGCVGGHHAVSLSAQAIRNGEADIAFACGHDFPILKPLIRTYSDPKARALS
                     IESENPKEAMKPYDRRRDGFSLGEGCIVICMEKLEHAQARNAKIYAEVLSYYNYNEAD
                     HPLHMDLSGRRLSEGMEKVLELGDMTAADISYFCGHGTSTHYNDLSESRAMRQYYKIQ
                     KTTKWAPLGSLKPIYGHIFGASGIVNVAGSALMIHNQTLCPTINHKTPDPECDHDHVA
                     EGKRPGKVNLALSLAYAIGSQTSLVCLAKV"
     gene            complement(2312..4405)
                     /locus_tag="PK683_01335"
     CDS             complement(2312..4405)
                     /locus_tag="PK683_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07114.1"
                     /translation="MKITIKVSLVSFAALIILLSGGLISFISFIGVQRTTYLLSEELM
                     ENICTHIIDRTLSHMQAATISAEISEFMMKKDPLSESKRESLDRYFRSILNANPQVAS
                     VYFGNEKDGRFLMFERQKDGSISENYMAPKSGKLMVSWKHENPEFYKTAPELKEESAE
                     YYDPRTRPWYRQAAEKGDHAWTDIYLFVPDNVPGLTHSNPVYADGKLEGVFGLDVGMK
                     TLSYFLGEQKIGKTGKAFFINNRKEIIAHPFLKEEKFHSIDENNPNKEDAEIVRSVQN
                     FRKKVKKKSWTDLKTKPIFFSYKSEGSTMMNMYLALQNRDFDWMIGIVVPEDDYVYLI
                     KQNNRIIWITSLVLMVFAVGAGLIFARRISEPLSVLEEEMKLVKNFDMNSDREIHSFL
                     REVDNMAVSFHGMKQGLRSFQKYVPDDLVRELISLGREAVPGGERKKITIYFSDIAGF
                     TSVSERLSPEALVVLLGEYLETCSQKIIEHRGTIDKYIGDSIMAFWGAPKPLEDHPLY
                     ACRAALSVNESLAELGKKWAAEGKPVLTQRIGIHTGEVIVGNFGSSTRMNYTAIGDGV
                     NLASRMEGLNKFYGTEILISEAVHEEVKNFMIARKTDMAAVKGKLESTSIYELICSRE
                     NADSDLLEWIELFHNGFSLYVKKDWNGALRLFRKVLKIKKNDSVSKIFIERCELFKKH
                     PPDKDWNGVFQMSSK"
     gene            4537..4740
                     /locus_tag="PK683_01340"
     CDS             4537..4740
                     /locus_tag="PK683_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07115.1"
                     /translation="MSHNTASISACRIQLNGGKIALKSDNGKYLARCRNCSPGAAYED
                     TVTVHADDPENQAYAQRDMSFLP"
     gene            4763..4924
                     /locus_tag="PK683_01345"
     CDS             4763..4924
                     /locus_tag="PK683_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07116.1"
                     /translation="MLAGNQHNPSLNLSDLVFVGISHIHKCVVAPDAGTLEALQDSLC
                     SDAELQDSH"
BASE COUNT         1312 a         1144 c          996 g         1479 t
ORIGIN      
        1 ccggtcagcc gattggtttc ttcaatgaag gaatagtcat caagatatgt ttcaagtccc
       61 aaagaggcaa agaaaggctg atcgggcaca cggagagact gaggatgagt gccgaaagga
      121 atttcaacga ctccgagcac atttgttccg gaaacactga tgagagagga cggaatcata
      181 cctgcaggaa cgatcttttc tgcagaaaca agaatacctc tttttgcagc cagtgctccg
      241 tagaaggatt ccccgcccgg ttcagacaat acgaaatttc cttcttcatc tgcgactact
      301 gaatgaatca gtgtgatgtc agggttcatt gccttcagaa gaagaatttc ttttccttct
      361 gaatcatttt gaacgaaagc cgtttttccc agtttgtcac tgatcatatc agaacccaga
      421 agagagttcg atacagtgta ttccagtttc atcgaacctg ccatcagtct ctgtacgaag
      481 ctccagagcg accagagttc cagctcaaac ggatttcctt tccatacatt ttcgtaaagt
      541 ctgtttggaa taggtctggg ataattgtct cccagaaatc cggtaatcat tctggaacag
      601 attccagaca gtgcaattgc atttgcattg gaatgaatgc ctgtgacact gacagtaaac
      661 tcaggcttct gatgataaaa aactctgcag agctgattga tcagaagatt cggcctggac
      721 atcactgcgg agagatggat atgatccttc ttccgaacaa attttttcag aagctcttca
      781 gggtttttga atactctgcc catcagactt ttgcgaggca gaccagggaa gtctggcttc
      841 ctattgcata tgctaaagag agtgcaagat ttacttttcc gggacgtttt ccttctgcca
      901 catgatcgtg atcacattca ggatcgggag tcttgtgatt gattgtcgga cagagagtct
      961 gattatgaat catcagtgca gatccggcga cattgactat tcctgatgca ccgaatatat
     1021 gaccgtatat cggtttcagc gaacctaaag gagcccattt tgtcgtcttc tgaatcttgt
     1081 agtactgacg cattgcgcgg ctttcagaca gatcattgta atgagtggaa gttccgtgtc
     1141 cgcagaaata gctgatatct gcggcagtca tatcgccaag ttccagcact ttttccattc
     1201 cttcagaaag tcttcttccg gagagatcca tatggagagg gtgatctgct tcattataat
     1261 tgtaatagga cagaacttct gcgtagattt ttgcatttct ggcctgagcg tgttccagtt
     1321 tttccataca gatcacgatg cagccttctc ccaatgagaa cccgtcccgt cttctgtcgt
     1381 agggtttcat tgcttccttt ggattttcac tttcaatgga aagcgctcgg gctttagggt
     1441 ctgaatacgt tctgatgagc ggtttcagta taggaaaatc atgtccgcat gcgaaggcaa
     1501 tatccgcttc tccgtttcga attgcctgtg cagaaagact gactgcatga tgcccaccca
     1561 cacatccgcc ggaaatacaa gtgacaaatc cctgcacatt atatgcaagc gcagacaaag
     1621 tggctggatt ggaagccatt cctgtaagaa ttgcatgtct gttgaaacgg ctgtccattt
     1681 caggttctgt ataatgaata cggtaagcct catcccacca gtacatagaa gatctggagg
     1741 atgaatcaaa cagttgaact ctctccggct cccagccttc ataatccaga cggctgtctt
     1801 caagacattt tcccaaggta ttcatcagaa caagcgtctc atagctgtac ttgtccacaa
     1861 atttagggtc aaggcgggga ggaaggttga ttttaggatc aaaacccttc agttcagcgg
     1921 caactttgac agaaagatca tcggtaggga accgtgtaat aaaatcaatc tgtgactgac
     1981 cttcggagat gtttttccag aaatcatcaa cagtcagagc accaggcaag actgtaccga
     2041 tccctgtaat tacaactcta ggcttagcca aaagtttttt ctttcgggct attaggttta
     2101 gtcgtctttc gacccatctg ctcatattta ggttttcatc atttttggtg aaccttaaat
     2161 taatgcaagt aaattattgt accttgaaaa tgtcataatt tgagctctta atttctcagt
     2221 tttcatcttg aggaaaaaaa ttaggactgc agaaaatact gaatttctgc ctccagaaac
     2281 caagtaaaaa tacttaaaaa aagacaacag tctactttga actcatttga aaaacaccgt
     2341 tccagtcttt atcaggagga tgttttttaa aaagttcaca gcgttctata aaaattttgg
     2401 aaacactgtc gtttttcttt attttcagca ctttcctgaa aagacgcaat gctccgttcc
     2461 agtctttttt cacatataat gaaaatccat tatgaaacag ttctatccat tcgagcaggt
     2521 ccgaatcggc attttccctg ctgcagataa gttcatagat ggaagtggat tccagctttc
     2581 cttttacggc cgccatatct gtctttctgg caatcataaa attctttact tcctcatgta
     2641 cagcctcgct gatcagaatt tcagttccgt agaatttatt cagtccttcc attctgctgg
     2701 caagattgac cccgtctcct attgcagtat aattcattcg tgtgctcgaa ccgaaatttc
     2761 cgacgatgac ttcgcctgta tgaattccaa ttctctgtgt cagtacaggt tttccttcag
     2821 ccgcccattt tttcccaagt tcagcaaggc tctcgttgac ggacagagcc gcccgacatg
     2881 cataaagcgg atgatcttcg agaggcttag gagctcccca aaaagccatg atggaatcgc
     2941 ctatatattt gtcgattgtt cctctgtgtt caataatctt ctgactgcag gtttccagat
     3001 attccccaag aagaactaca agcgcctcag gagaaagtct ttctgaaaca gacgtgaaac
     3061 ctgcaatatc tgagaaatat attgtaattt tttttctttc accgcccgga acagcctccc
     3121 tgccgagaga aatcagctcg cgcaccagat cgtcaggaac atatttctga aatgatctca
     3181 gtccctgctt cattccgtga aaggaaacag ccatattgtc cacttctctg agaaaagaat
     3241 gaatttctct gtcagagttc atatcaaagt ttttcacaag cttcatttct tcttcaagca
     3301 cagaaagagg ctctgaaatc cttctggcaa atatcagacc tgctcctaca gcaaacacca
     3361 tcaaaacaag cgatgtgatc catataatcc tgttgttctg ctttataaga tatacataat
     3421 cgtcttccgg aacaacgatg cctatcatcc agtcaaaatc tctgttctgc agagccagat
     3481 acatattcat cattgtgctg ccctcagatt tataggaaaa gaaaatcggc ttagtcttca
     3541 gatcagtcca gcttttcttc ttcacctttt ttctgaaatt ctgaactgat cttacaatct
     3601 ctgcatcctc tttgttcgga ttgttttcgt cgatggaatg gaacttttcc tccttcagaa
     3661 agggatgggc tatgatttcc tttctgttat tgataaagaa ggcttttcct gtctttccaa
     3721 tcttctgctc tcccagaaag taggacagag tcttcatccc cacatccaga ccgaacactc
     3781 cttccagttt tccgtctgca tacacgggat tgctgtgggt cagtcccggc acattgtcag
     3841 gaacaaaaag ataaatgtct gtccacgcat gatcgccttt ttccgctgcc tgtctgtacc
     3901 aagggcgtgt tctaggatca tagtattcgg cggattcttc tttcagttcg ggggcagtct
     3961 tataaaattc cgggttttca tgcttccagg aaaccatcag ttttccactt ttcggagcca
     4021 tataattttc gctgattgat ccgtccttct gtctttcgaa catcagaaac cgaccgtctt
     4081 tttcattccc gaaatatact gaagctacct gcggatttgc attcagtatg cttctgaagt
     4141 atctgtccag tgattctctt ttgctctcgc tcaaaggatc ttttttcatc ataaattcag
     4201 aaatctctgc actgattgtt gcagcctgca tatgggacag tgtcctgtca ataatatgtg
     4261 tacatatgtt ttccatcagt tcttctgaaa ggagatatgt tgttcgctgc actcctataa
     4321 atgaaataaa gctgatcaga cctcctgaga gaaggataat gagggccgca aaacttacca
     4381 gactgacttt gattgtaatt ttcatagact cagtttacac atcgtcaatt gcagcagcaa
     4441 ttctgaattc ctccacggtt tccgcagaaa ttgattatcg cttttcctga gtttttcttt
     4501 ttgtccaata ccggcagata tgcctgattg attcaattga gtcataatac tgccagtatt
     4561 tctgcctgcc gaattcagct gaacggcgga aaaattgccc tcaagtcgga caacggaaaa
     4621 taccttgcca gatgcagaaa ctgttctccc ggggcggcct atgaggatac agtcactgtt
     4681 cacgcagatg atccggagaa tcaggcatat gctcagcggg atatgtcttt tctcccctga
     4741 gacctgattc cagccagcag cagtgctggc tggaaatcag cacaatccct cactgaatct
     4801 ttcggatctt gtttttgtcg gaatcagcca catacacaaa tgtgtcgttg ctcccgatgc
     4861 aggaacactt gaagcgctgc aggacagtct ttgttcagat gcagaactgc aggattctca
     4921 ttgacctcat c
//
LOCUS       DAOQGJ010000048         4909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_8987_length_4909_cov_2.948290, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000048 DAOQGJ010000000
VERSION     DAOQGJ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8987_length_4909_cov_2.948290"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1356
                     /locus_tag="PK683_01350"
     CDS             196..1356
                     /locus_tag="PK683_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07117.1"
                     /translation="MNKHSVKFMNNPGSKPVFEQKPFTCFISRISASVLRLISFSFFL
                     ITVLYFGSDLQADTVVLKSGKAMDGKVVSQTADTLTFTAAGSPKKQIYPKKEIFRILY
                     NPTKEQRTQIINEALLFRKREIAKEKDPKAVDDEFIRLEEERTRQVEESLTSLQVKKE
                     EQAEEVEVRLERRIAQLEERLKDAEYFLELNTDWKDYYGIKRNKWDIVWRSALIPGWG
                     HSYAKNNFIGNVYFTTFFFSTIAYLAAGSAQRTAESTLNTKTTDLFLIRPLLYSAVLA
                     QTTSTGVSTFSSTQVTLINTYNDFQRLNDYRKLKNAVHSAETNQKTALNLVAGIYLIQ
                     LVHAYFTGKTWASREVLPEAKSGWNFQYLPQFNPMAMANNGFQAELRYFWNF"
     gene            1356..2492
                     /locus_tag="PK683_01355"
     CDS             1356..2492
                     /locus_tag="PK683_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07118.1"
                     /translation="MQIARMLKLLILFVFIISFQRCTKKKSSRCDAKTGVLGGLFLYL
                     NSCERGPSSQTDQLKKPFASTSIWNRLIPADAVYSDVSDAVSGDSAAFPAAVGPDIVA
                     ICYTDPAAPAVKVEKNIGWDYPGRGMSSGQVQYTRQLSASSCAGLSMVKNGNGLFAIV
                     DMKTGSADQGVGGWRCNGGSILHLLPDSTKAHISNVITGDGLQGYGRGSSLPSLGGVI
                     LQGEISSGINHAVAVTLPQTRFSKTKNYVWPASSADSIASNPDYGYQGPNTSYTMGTL
                     LAIPKNTDISSLGLSTPQARQLASAAQNYGWYIADASGYAKGKIPSVQFAMEAWAAKN
                     DLGFTVDYSTEKNSIDKSKLDDSLFTADIQKILKNLKAVTSNTQ"
     gene            complement(2486..3667)
                     /locus_tag="PK683_01360"
     CDS             complement(2486..3667)
                     /locus_tag="PK683_01360"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07119.1"
                     /translation="MYDFMNELISLKNMVTVLQTTLDNIGAYIYIKDLEGKYIFVNRL
                     VKELFDAPLEEIIGFDDSKFFSLEKSDDLRVNDRLVMDQGQTIEKEEKNHVIKTGEIK
                     YYWTVKKPRRDENGKITGMYGISTDITQTKKLENEVNQKNAELLRYLEIIHSDLNTAK
                     NIQKNLLPKNLDLEAGLDISVRYHPLSEVGGDIYDVHRLRNDTIRIFLADATGHGIQA
                     ALMTMSIHSEYLSIKDYILPPGRILTILNTRYFRRYSHLNSFFSCLILDIDLKEGIIS
                     YSSAGHPDQILYNSERQIRLKSTGKIIGVLENVEYGTREENFGNSDRLCLFSDGIYEM
                     INPDGKEFGEDKFFALADELKNLPAPSFLDNIIRRVFAFLGIGPQQDDITLISVQRKT
                     Y"
     gene            3842..4582
                     /locus_tag="PK683_01365"
     CDS             3842..4582
                     /locus_tag="PK683_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07120.1"
                     /translation="MDTVYIMKTLIHRIIHFFLPLKCRICGDKDRMSSLAGICRGCSR
                     GKFAFRKRKGLCSTCSGILTDAKCEFCTGRNVFFEKLEYIAEKGEFESDLIRRLKFHS
                     SPEIGNLFLFRLGGISGRLKTVSDEWNICYVPSSAKTRRERPYFSFSSVMNFIRKKTG
                     AKTVPALEKKSKELQSGKRFRDRFIHARFSMQIRSRFRNSLSGNFLLIDDVFTTGATL
                     NEAARILMENGAEKIYCIVMLKSFIEDN"
     gene            complement(4586..>4909)
                     /locus_tag="PK683_01370"
     CDS             complement(4586..>4909)
                     /locus_tag="PK683_01370"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNH07121.1"
                     /translation="EPDADRIIESAEKLQEAGVFSIVLEMVPEDLGKKMTESVDVPII
                     GIGAGRYVSGQVLVLYDLLGINSSFNPRFLKKYADLNSVILNALNAYSFEVKDSSFPS
                     EKNVF"
BASE COUNT         1451 a          969 c         1062 g         1427 t
ORIGIN      
        1 agctgataat gacgttagtt tactgagtaa tttctgtgaa atgattttcg gggctgaata
       61 gatctcgtta tatttcatca actggaattt ctgcacatat taaatataat atgatgaatc
      121 acagtgaatt cggtcgggtc tgcttgagtt tttggagaga aatactgcat aaaggaaaat
      181 tgcatgaaaa gttttatgaa taagcattca gtaaaattta tgaataaccc aggttccaaa
      241 cctgtttttg aacagaaacc atttacttgt ttcatcagcc gaatttctgc ttcagttctt
      301 cggctgatct cattctcatt ctttctcata actgttctgt atttcggatc agatcttcag
      361 gcggatacag ttgtactgaa atcaggaaag gcgatggatg ggaaggttgt atcccagaca
      421 gcggatactc tgacatttac tgctgcagga agtcctaaaa aacagattta tcctaaaaag
      481 gagatattcc gtatcctgta taaccctacc aaggaacaga gaacacagat tatcaacgag
      541 gcccttcttt tccgcaaaag agaaattgca aaagaaaaag atcccaaagc agtggatgat
      601 gaattcattc ggcttgaaga agaaagaaca agacaggttg aagaaagtct cacttcactc
      661 caggtaaaaa aagaagagca ggctgaggaa gtggaagtgc gtctggaaag aagaatagca
      721 cagctggaag agaggctgaa ggatgctgaa tattttctgg aactgaatac tgactggaag
      781 gactattacg ggatcaaaag gaataaatgg gacattgtat ggagatctgc tctgattcca
      841 ggctggggac acagttatgc aaagaataat ttcataggca acgtctattt tactactttc
      901 tttttcagta cgattgcata ccttgcggca ggatctgccc aaagaactgc agagagcaca
      961 ctgaacacca aaaccacaga tctttttctg atccgtcctc ttctgtacag tgccgttctt
     1021 gctcagacaa catcaactgg tgtttccacg ttttcttcaa cccaggtaac tctcatcaat
     1081 acatacaatg attttcagag actcaatgat tacaggaagc tgaagaatgc agttcattct
     1141 gcggaaacaa atcagaaaac agcattgaat ctggttgcag gaatttatct gatccagctt
     1201 gttcatgcct atttcacagg aaaaacctgg gcatccaggg aagttcttcc agaggccaaa
     1261 tccggatgga atttccagta tcttccgcag tttaatccta tggctatggc caataacggg
     1321 tttcaggctg aattgaggta cttctggaat ttctgatgca gattgccaga atgctcaagt
     1381 tacttatatt atttgtattt attataagtt ttcagagatg cacaaagaaa aaatcctccc
     1441 gctgtgatgc aaaaacaggt gtcttaggag gactgtttct ttatctgaac agctgcgaga
     1501 gagggccttc ttcacagact gatcagctga aaaaaccttt tgcttccaca tcaatatgga
     1561 acagactgat cccggcagat gctgtctatt cagacgtttc agatgcagtg tccggtgatt
     1621 ctgctgcatt tccagctgca gtcggtccgg atattgttgc aatatgttat actgatcctg
     1681 ccgcgcctgc agtaaaagtg gaaaagaata tcggctggga ttatccagga agaggaatga
     1741 gttccggaca ggtacagtat acaagacagc tttcagcatc ctcctgcgca gggctttcca
     1801 tggtgaaaaa cggaaatggt ctttttgcaa tcgtggatat gaaaacagga agtgcagatc
     1861 agggagtggg gggatggaga tgcaatggag gttcgattct tcatcttctc cctgattcga
     1921 cgaaggccca tatcagcaat gtaattacag gcgacgggct tcagggatac ggaaggggat
     1981 caagtctccc ttctctggga ggggtgattc tgcaggggga aatcagttcg gggatcaatc
     2041 atgctgttgc agttactctt cctcagacaa gattttccaa gacaaaaaat tatgtatggc
     2101 ctgcatcctc tgcagacagc attgcatcca atcctgatta cgggtatcag ggtccgaata
     2161 catcgtacac tatgggaacg cttcttgcaa ttcccaaaaa cactgatatc agttcactgg
     2221 gactctctac gcctcaggca aggcagctgg catcggctgc gcagaattac ggatggtaca
     2281 ttgcggatgc gtccggttat gcgaaaggaa agattccttc agttcagttt gcgatggagg
     2341 cctgggcggc gaaaaatgat ctaggattca ctgtggacta cagtactgaa aaaaacagca
     2401 tcgacaagtc caagctggac gattcgcttt ttactgcaga tattcagaaa attctgaaaa
     2461 acctgaaggc agtgacttca aatactcagt aagtttttct ctgaaccgat atcagtgtga
     2521 tatcatcctg ctgagggcct attcctaaaa aagcaaatac tctgcggatg atattgtcta
     2581 aaaaactggg agcaggcaga tttttcagtt catctgccag tgcaaaaaat ttgtcttctc
     2641 cgaattcttt tccgtccggg ttgatcattt catatatgcc gtcagagaaa agacagagtc
     2701 tgtcggaatt gccgaaattt tcctctctag tgccgtattc tacattttcc agcacaccaa
     2761 taatcttgcc tgtgcttttc agtcggatct gcctttcgga attgtaaaga atttgatcag
     2821 gatgtcctgc agaggaatac gaaatgattc cttccttcag atctatgtcc agaatcaggc
     2881 aggaaaaaaa agaattcaaa tgagaatatc ttctgaaata ccgtgtattt aaaattgtga
     2941 gtattctgcc gggagggaga atataatcct taatgctcaa atattctgag tggatggaca
     3001 tagtcattag tgccgcctga attccgtgac cggttgcatc tgcgagaaaa attcgtattg
     3061 tatcgtttct cagtctgtgt acatcataga tatctcctcc gacttcactt agagggtggt
     3121 atctgactga aatatccagt cctgcctcaa gatccaggtt tttgggaaga agatttttct
     3181 gaatattttt tgcagtgttc agatcggagt gaatgatttc aagatatctt aaaagttcag
     3241 catttttctg atttacttca ttttccagtt tctttgtctg agtgatatca gtggatattc
     3301 cgtacattcc agtaatcttg ccgttttcat ctcggcgcgg tttttttact gtccagtagt
     3361 atttgatttc tcccgttttt atcacatgat tcttttcttc tttttctatt gtctgtccct
     3421 gatccatgac aagcctgtcg tttacacgca gatcatcgga tttttccaat gaaaaaaatt
     3481 tactgtcgtc aaacccgatg atttcttcca aaggtgcatc gaagagttcc ttcacaagcc
     3541 tgtttacaaa aatgtatttt ccttccagat ccttgatata gatatatgct cctatattgt
     3601 ccagagtggt ctggagcaca gtcaccatat ttttcagaga aatgagttca ttcatgaaat
     3661 cgtacatagt gcgagaaagg acataatata tatgtctgaa taatcagtaa agaaaaaaat
     3721 aattgaaaaa gcttttcgga gccgatcttc catattttga tctctgctga aaacagccgt
     3781 cctaaggcaa aaaaaacagg taagatgaaa aatcggaaat aaaaatgccg tttgattcag
     3841 aatggataca gtgtatatta tgaaaactct gattcatcga attatccatt ttttccttcc
     3901 tctcaaatgc agaatctgcg gtgacaagga caggatgagt tctcttgccg ggatctgcag
     3961 aggctgttcc aggggaaaat ttgctttcag aaaaaggaag ggtctttgca gcacctgttc
     4021 cggaattctg accgatgcaa aatgtgaatt ctgtacaggc agaaatgtat tctttgagaa
     4081 actggaatat attgcagaaa aaggagaatt tgaatcggat ctgattcgca gactgaaatt
     4141 ccattcttcc cccgagatcg gaaacctttt tctgttcagg cttggcggaa tttccggaag
     4201 gctgaagaca gtttccgatg agtggaacat ctgttatgtt ccgtcttctg caaagacaag
     4261 aagagaaaga ccttatttca gtttcagctc cgtgatgaat ttcatccgta aaaagaccgg
     4321 ggcaaaaact gttcctgcgc tggagaaaaa atcaaaggaa cttcagtcag ggaaaagatt
     4381 cagagacagg ttcattcatg cgagattttc catgcagatt cggagcagat tcagaaattc
     4441 cctttccggg aactttcttc tgattgatga tgtttttact acgggggcta cactgaatga
     4501 agcggccaga atcttaatgg aaaacggtgc agaaaaaata tactgtattg ttatgcttaa
     4561 gagttttatc gaagataatt agcagttaaa acacattttt ttcagaagga aaagaactgt
     4621 cttttacttc aaaggaatat gcattcaatg cattcaaaat cacagaattc aggtccgcat
     4681 attttttcag aaacctggga ttgaaagaag aatttattcc cagcagatca taaaggacga
     4741 gaacctgtcc ggaaacatat cgaccggcgc ctatcccgat aatgggaacg tctacagatt
     4801 ctgtcatttt ttttccgaga tcttccggca ccatttccag cactatggaa aatacgcccg
     4861 cttcctgaag tttttcggct gattcaatga ttctgtccgc atccggttc
//
LOCUS       DAOQGJ010000049         4888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9045_length_4888_cov_3.253052, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000049 DAOQGJ010000000
VERSION     DAOQGJ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9045_length_4888_cov_3.253052"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2920)
                     /locus_tag="PK683_01375"
     CDS             complement(<1..2920)
                     /locus_tag="PK683_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH07122.1"
                     /translation="MKSIGKFIVEKTLIIQFVLVLLTLIGFSRMMSMHREAFPNVALD
                     KVVVEAPLPGATPEEIERLIAIPIEKKLRSVTNLEKIRSFNLENASVIMIFLKEGVKN
                     SKKVVDDIKAAVESTRLPDNAVKPTVREITTEKQEVINIALSLRQNGKDSTEDYRKLR
                     NTAKAFEDRLIQLKDVADIEKFGYRNREFLVEVRPDALNAREIGLNTVLSVLGSRNIN
                     IPSGTMKINGTEYLLRTKGDFEEAKDMVNLPILGNEIGYATLLKDVAKVSDTFEEEKI
                     FEKLNGKHGIILRVWKNEQADIITTADSVKKLIESIQNNYPEIETSVYEDKSRDVRRQ
                     LGDLVLNFETGLVLVLLSLLFILGVRLGVMISVAIIFIFLIAFIFIKQFEFTINTITI
                     FGMVMVLGMMVDNSIVVAENTFRLMQEGYSRKDAILQTFKDVLVPLLVSFLVISAAFL
                     PLLFMSGIIGKFIVGIPSVVLITLASSLLFALVFLPNWLNVFLPEKLKISPKEESIEK
                     KEGIFGIVIPGYKKTVGFALKHRGLVLIAFNIVFFLTIFAAGKFLPFIMFPPGSEEDI
                     EIKTWMPIGTTLQKNRETMDIIEPAVISMAGNDFIYIRSRIGIHESPIVDPKPGQEVH
                     RSHLTLKITPAADRKEWKDARLLVKKIREYMDEAKKTGLISRDMFYDVNARIKGPPVG
                     KPVSLEIRGPEYKSIKEIADLYINEIKKMDGVFDIRIDLEQGKEEFRFYVKDEAASRT
                     DVSARDISRTVRTAYNGEIASSISKGEDKINIMVRFPEAERLSMKSLSKVKVENRNRR
                     LIPLDQVAYYAKEREYSMINRQDLLRVVRLEASVDTKKTTSLSVNRKLQSMIKLDKYP
                     EYSVVFGGEQEDAQKSFKDLAISMLFAVAVIFGIFIIYFNSVSTTAVIIGSIPFGIVG
                     VLFALMTHGMPLSFMSTLAIVALSGSIVANTLILITFIEELRAGGMPLDEAIINGGAI
                     R"
     gene            3163..3729
                     /locus_tag="PK683_01380"
     CDS             3163..3729
                     /locus_tag="PK683_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH07123.1"
                     /translation="MENLKKEILDKAETLFCQFGPSRVTMEEIAHEFGISKKTLYQFY
                     SSKNDLLKEVTDSIYSQIQDRNQSLLKNTEMNFLEKINKLIKSSLENISRTEILKKDL
                     QKNFPDLWKHYSNLHSQDYPEYLEKLLKEGIAKGQIRKDINIPIVLMIFSSCLESLAI
                     NPVKNSDHPAKDLVKEIHTVIFYGLIKR"
     gene            complement(3732..>4888)
                     /locus_tag="PK683_01385"
     CDS             complement(3732..>4888)
                     /locus_tag="PK683_01385"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07124.1"
                     /translation="LSEAVKLLNETIVLRRELRKDEPVLDIYSLLIETYIDSNELGRA
                     EQNLLLAEKEIPKSKDKETLKAFYKTASLLSEKKGKTKEALQYFKKYFEMDKSIYNES
                     LQEQFAQMQTKYESEKKQKENEILKKQTEIQKLNIERQSIVIVCTVIGLILLLTLAVL
                     IYRGYREKKKDNEIIAAEKEKSEKLLLNILPEAVAADLKENGSTLPRIFNNVTVFFSD
                     IVTFTDISSRMEPDVLISELNELFTAFDDIIEKNGCERIKTIGDAYLAVCGMPEENEN
                     HAENMIRAAKEIIQYLTKRNENGSFQWKIRVGIHSGKVVGGVVGVKKYIYDVFGDTIN
                     TCSRMGSNSLPMKINVSEETYSTVKDKFNFTRRKPQEVKGKGKVRMYFVE"
BASE COUNT         1306 a         1060 c          926 g         1596 t
ORIGIN      
        1 gtcggattgc tcccccgttg atgattgctt catccagagg cattcctcct gctctcagct
       61 cttcaatgaa tgtaatcaga atcagtgtgt ttgcaacaat gcttccgctg agtgcaacaa
      121 tggccagcgt gctcataaaa ctcagaggca ttccgtgtgt catcagtgca aaaagaactc
      181 ccacaattcc gaaaggaatg gatccgataa tgaccgcagt tgtgcttaca gaattaaaat
      241 aaatgataaa aataccgaaa atcactgcaa cagcaaagag cattgatatt gccagatcct
      301 tgaatgattt ctgagcatct tcctgttcgc ctccgaatac tactgagtat tcagggtatt
      361 tatccagttt gatcattgac tgaagttttc tgttcacaga aagagaggtt gtcttcttgg
      421 tatccactga agcttccaga cggacaacac gcagaagatc ctgtctgttg atcatagaat
      481 actcacgttc ttttgcatag taggctacct ggtcaagggg gatcagtctt ctgtttctgt
      541 tttccacttt gaccttgctc agacttttca ttgaaagtct ttctgcctcg ggaaaacgca
      601 ccataatatt gatcttgtcc tctcccttgc tgatgctcga tgcaatttca ccgttgtatg
      661 ctgttctcac tgtcctcgaa atatctcttg cacttacatc tgttctgctt gctgcctcat
      721 ccttaacata aaatctgaat tcctccttcc cttgttcaag atcaatgcgg atgtcaaata
      781 caccatccat tttctttatt tcatttatat ataaatctgc aatttcctta atggatttat
      841 attccggccc tctgatttcc agacttaccg gttttcctac tggaggacct ttgattctgg
      901 cattcacatc gtagaacata tccctgctga tcaagcctgt ttttttggcc tcatccatat
      961 attctctgat ttttttgaca aggagacgtg cgtccttcca ttcctttctg tccgctgcag
     1021 gggtgatctt aagtgtcaga tgtgaacgat gcacttcctg accgggctta ggatcaacga
     1081 ttggactttc atgaattcct atcctacttc tgatatagat aaaatcgttt cctgccattg
     1141 atataactgc aggttcaatg atatccatag tttctctgtt tttctgaagc gtggttccta
     1201 tcggcatcca tgtctttatt tcaatgtctt cttcgcttcc aggcgggaac ataatgaacg
     1261 gcagaaattt ccctgctgca aaaattgtaa gaaaaaagac aatgttgaat gcgatcagaa
     1321 caagccctct gtgtttcagt gcaaaaccta cagtcttttt gtaacctgga atgactatgc
     1381 cgaatattcc ttccttcttc tcaatggatt cttcctttgg agaaattttc agtttttcag
     1441 gaaggaatac attcagccag ttcggaagga aaacaagagc aaaaagaaga gaacttgcca
     1501 gagtaatcag gaccactgag gggatgccta caatgaattt gccaatgatt ccgctcataa
     1561 aaagaagagg aaggaaggcc gctgaaatca caagaaagga aacgagcagg ggaacaagca
     1621 catccttgaa agtctgcagg attgcatctt ttctggaata tccttcctgc atcagccgga
     1681 acgtgttctc tgcgacgaca atggagttgt ccaccatcat tcccagaacc atcaccattc
     1741 cgaaaattgt aattgtattg atagtaaatt cgaattgttt tatgaaaata aatgcaatca
     1801 gaaagatgaa tataatggca acgctgatca tcacaccgag tctgacgcct agaatgaaca
     1861 gaagtgagag aagtaccagc actagacctg tttcaaaatt gagaacaaga tctcccagct
     1921 gacggcgtac atcgcggctt ttgtcctcgt agaccgatgt ttcgatttca ggataattat
     1981 tctgtattga ttctatgagt ttttttacag aatctgcggt tgtaatgatg tctgcctgtt
     2041 cgttcttcca gactctaagg atgattccgt gttttccgtt cagcttttca aatattttct
     2101 cttcttcaaa tgtatctgaa acttttgcaa catccttcag aagagtggca tatccgattt
     2161 cgtttccgag aataggcaga tttaccatat ccttcgcttc ctcaaaatcc cctttcgttc
     2221 tgagaagata ttcagtaccg ttgattttca tggttcctga gggaatgttt atgtttcgcg
     2281 atccaagaac tgacaatact gtattcagac ctatttctct tgcattcaga gcatccggtc
     2341 tgacttctac cagaaattca cgattcctgt atccgaattt ttcaatgtct gccacatcct
     2401 tcagctgtat gagcctgtct tcaaaagcct tggcagtgtt tcgaagtttt ctgtagtctt
     2461 ctgtcgaatc ttttccgttc tgcctgagac tgagtgcaat attgatgact tcctgttttt
     2521 ctgtcgtgat ttctctcaca gtcggcttaa ctgcattgtc aggaagtctt gtggattcca
     2581 ctgcagcctt gatatcatcc acgacttttt tggagttctt tactccttct ttcagaaaaa
     2641 tcataattac gcttgcattt tccagattga agcttctgat tttttccagg tttgtgacgg
     2701 atctgagttt tttttcgatc ggtattgcaa tgagcctttc tatctcttct ggcgtggcac
     2761 cgggcagagg ggcttctacc actactttgt caagtgcgac attcggaaag gcttctctat
     2821 gcatactcat cattctgcta aatccgatca gagtaaggag aacaagtacg aactgaataa
     2881 ttagtgtttt ttcgacaata aacttaccga tgcttttcat gctgatcctg ccttgcagac
     2941 aataattttc ggctgaatac tgtcctgcct gcaatgactt gactgtaaag acagtatgga
     3001 aaccatgtaa acaatatcag tttccgaagt tttaaggtca agttttttta gcctcagtct
     3061 gaaaagagtt cgacttttat gcgattaaat ttaaaaactg ttgaaaagat ataatataag
     3121 ggaataattc cttcttcagg aagactgtaa agtgatatac gaatggaaaa tttaaagaag
     3181 gaaattctgg acaaggcaga gactctgttc tgtcagtttg gtccatccag agtgacaatg
     3241 gaagagattg cccatgaatt cggaatcagc aaaaaaactc tttatcagtt ttattcatcc
     3301 aaaaatgatc ttttaaaaga ggtaacagat tccatataca gtcagattca ggacagaaat
     3361 cagtctctcc tgaaaaatac agagatgaat tttctcgaga aaataaataa acttataaag
     3421 tcatcccttg agaatatttc tagaacagag attctcaaaa aagatctgca gaagaatttt
     3481 cctgatctct ggaagcacta ttccaatctg cattctcagg actatcctga atatctggaa
     3541 aaacttctga aagagggaat tgcaaaggga cagatccgca aggatatcaa tatcccgatc
     3601 gtgctgatga ttttcagttc ctgtctggaa agccttgcaa tcaaccctgt aaaaaattcg
     3661 gatcatcctg ccaaggatct ggtaaaggaa atccatactg ttatttttta cggtctaatt
     3721 aaaagataga gtcattccac aaaatacatt ctgacctttc cctttccctt gacttcctgg
     3781 ggctttcttc gtgtaaagtt gaatttgtct tttactgttg agtaagtctc ttcggataca
     3841 ttgattttca tcgggagaga attgcttccc atccgagaac aggtattgat tgtgtcaccg
     3901 aatacatcat atatgtattt cttcacacct acaactccgc ccacgacttt tcctgaatga
     3961 attccgaccc tgattttcca ttggaatgaa ccattttcgt tccgtttcgt aagatactgt
     4021 atgatttctt ttgcagcgcg aatcatattt tctgcatggt tttcattttc ttccggcatt
     4081 ccgcacactg ccagataggc atctccgata gtcttgattc tttcgcatcc gttcttttcg
     4141 ataatgtcgt cgaatgcagt gaaaagctca ttcagttccg agatcagcac atcaggttcc
     4201 atcctggagg aaatatcggt aaatgtgaca atatctgaga agaatacagt tacattattg
     4261 aatattctag gaagtgtcga accgttttct ttcagatcgg ccgccacggc ttcgggaagt
     4321 atgttcagga gaagtttttc cgatttttcc ttttctgccg caatgatctc attatctttt
     4381 ttcttctctc tgtagcctct gtaaatcaga acagcaaggg tcagaagcag tatgagaccg
     4441 attactgtgc acacaatcac gatggactgt ctttctatat tcagtttctg aatttctgtc
     4501 tgctttttca gaatctcatt ctctttctgc tttttttctg attcatactt tgtctgcatt
     4561 tgagcgaact gttcctgcag agactcattg taaatagact tatccatttc gaagtacttc
     4621 ttgaaatact ggagagcttc ttttgtcttt cctttttttt cactgagaag ggatgctgtc
     4681 ttataaaaag ccttcagtgt ttccttgtct tttgatttgg gaatttcctt ctcggcaaga
     4741 agaagattct gctcagctct cccgagttca ttgctgtcga tatatgtttc gatcaggaga
     4801 ctgtatatat ccagtacggg ctcatccttt ctcagttccc tcctgagcac aatcgtctcg
     4861 ttcagaagct tgactgcctc cgaaagcc
//
LOCUS       DAOQGJ010000050         4878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9070_length_4878_cov_3.240722, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000050 DAOQGJ010000000
VERSION     DAOQGJ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9070_length_4878_cov_3.240722"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PK683_01390"
     CDS             <1..1156
                     /locus_tag="PK683_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNH07125.1"
                     /translation="TTTVTTETVKKITELQDRIAVLESEKDKLQKEKDALIKEKGNIP
                     ALEAKIQELEAKIAQMEEDKKNQAKQNSENSDALTQLNKKLDDLQAEKEQIQKEKDQA
                     VNDSNSEKEDLLAKFAQMEVKLKQLEDEKTELEQKLKECDEEKGKLQTANKALEETVE
                     KLKTELAEAGDSKEKQEKELIELKKQIEELQNQLAAQKTDNEAAVADLAAMKDKHAEE
                     TQKYKDEIEKLTAQTQKLEEALENEIKKGDSTVVNKNGNIIINLSDDVNFATASAELT
                     SQGKKTLDKIYNVVKKYPNSHINIEGNTDDRPISTAKYKDNWELSTARAVAVVRYFVH
                     HKKMNPKQFAAVGYGEFNPLKPNNSDKNRLMNRRVEIVVKPIKKAVKEQP"
     gene            1342..2973
                     /locus_tag="PK683_01395"
     CDS             1342..2973
                     /locus_tag="PK683_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNH07126.1"
                     /translation="MSEKILKLSAIIVLLNMSHLSAEDVGPSPGTPQAPAEEKEPGDL
                     FYYKSEYTDLWIKKRTLEKEVAELKAKQNAADKRSMDENAANKTRLSELETELAAVNQ
                     KYSELYDKYQTYVRENEAKLSSMDNSIVSLRSQKEAAERRYLAVKEEKEVLERELLAI
                     RNQKDGLDKNSLALKEQNALLEKKLKEQNLLLEQKNRELAKAEKTAREYNESILEKDS
                     ISQEQAIIYRDTISKIEQEKTSLQEKNNRLTEEVERLKEINRAYLQRLEEANARLQEL
                     ETSKRALQKKLEDSKSDEAMLNSLRKENAEISYQRDTLLKELDDMRKSVTDRSAELDR
                     ELSSLQKDYNAREKQLEAELERLRQRETELLAENSRLIAANDEIAYENLRLKEQLESI
                     NRARKEMEKSFENEIRTGELDVNQNKKGVVIGIPLNVTFDTYSSRIRPEGRKILDKII
                     RILNTYKIKKITIEGNTDDRPVLKTSKYRDNWELSTARAISVLRYLVRYSDIHPRFMT
                     AAGNAEYNPLKSNNSPRNRAANRRVEIVVTPAVTK"
     gene            2981..3706
                     /locus_tag="PK683_01400"
     CDS             2981..3706
                     /locus_tag="PK683_01400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH07127.1"
                     /translation="MEEEKGLFSEFFKVLANLDIGEEQASILWEEVLKNAQRLSEKVG
                     RKVGFRVALLDYMLNEKQIMKNPTFVEQDIFEEVMRNSFVDELTGIYNRRYFNTAFHR
                     EFKRSSRYEKTISLFFFDVDNFKSFNDNYGHLEGDRVLQLIAKTLSQLFRAEDIVARI
                     GGEEFAVLLPETGIESARTACERFAQKLKISSSEIMKREITVSGGIAAFPHSASTMDD
                     LYKKADELSYKAKRSGKDRIVTE"
     gene            3716..4060
                     /locus_tag="PK683_01405"
     CDS             3716..4060
                     /locus_tag="PK683_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07128.1"
                     /translation="MKNRFCISVFLYAREGSENIFLEYENAVMPLLKEHTGELLLRIR
                     PDRSSEADSKIRVPNEIHLIGFSEEKNFRSFLSDPVRKSLEHMRKESIVFSIVLQNAE
                     TCIPESFYLNNG"
     gene            4102..4767
                     /gene="lptC"
                     /locus_tag="PK683_01410"
     CDS             4102..4767
                     /gene="lptC"
                     /locus_tag="PK683_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNH07129.1"
                     /translation="MKDILFKYGSILEKMNIRCGYSFLNQILKQFLGRPSAPFFSGKN
                     ILYLFLFIFISAGCKKNKAERVEGDRESGSAITIRNYQRISVNDKGEMLWKLKAEETY
                     FFKEDNRTILYKIEADQLEKGKSKSRMKADKGYLDRKNEILKVSGNIYIRTNDGKELT
                     GEEMDYNIAKELLTSDKRVVIRTGKTVIRGRGLEADNSLNKFKIIHPEGVTVGTDNPF
                     KDN"
BASE COUNT         1739 a          895 c         1154 g         1090 t
ORIGIN      
        1 tactacaact gtcacaacag agacagtaaa aaagatcaca gaacttcagg acagaatagc
       61 tgtactcgaa tctgaaaagg acaaactgca gaaagaaaaa gatgcactga tcaaagaaaa
      121 gggcaatatt cctgctctcg aagcgaagat ccaggaactg gaagcaaaga ttgcccagat
      181 ggaagaggac aaaaaaaatc aggcaaagca gaactctgag aactctgatg cgctgactca
      241 gctgaataaa aaactggatg atcttcaggc agaaaaagaa cagattcaga aagaaaaaga
      301 tcaggctgta aatgacagca attctgaaaa agaagatctt ctggctaaat ttgcccagat
      361 ggaagtgaaa ctcaaacagc ttgaggatga aaaaacggag ctggagcaga aactcaaaga
      421 atgcgatgag gaaaaaggaa aactgcagac tgcaaataaa gctctggaag aaactgtaga
      481 gaaacttaag acggaattgg cagaagcagg agacagcaaa gagaagcagg aaaaagaact
      541 gatcgaactg aaaaaacaga tcgaggaact tcagaatcag cttgctgctc agaaaactga
      601 caatgaggca gctgtagcag atctggctgc aatgaaagac aaacatgctg aagaaactca
      661 gaaatacaag gatgagattg aaaagctgac tgcgcagact cagaaactgg aagaagctct
      721 cgaaaatgag atcaagaaag gcgattccac tgtagtcaat aagaacggca atatcataat
      781 caatctttct gatgatgtaa actttgcaac agcatctgca gagctgactt ctcaggggaa
      841 aaagactctg gacaagatct acaatgttgt caaaaaatat cctaacagtc atatcaatat
      901 tgaaggaaat acagacgaca gaccgatcag cactgcaaaa tacaaggaca actgggagct
      961 ttcgactgca agggcagttg ccgttgtgag atactttgtg catcataaaa aaatgaatcc
     1021 taaacagttt gctgcagtag gatacggtga attcaatccg ctgaaaccaa ataattcaga
     1081 taaaaacaga ctgatgaaca gaagggtgga aattgtagta aaacctatca aaaaagctgt
     1141 aaaagaacag ccttaacatt aaaaaacctg tcctcccgtc ggtggacagg tctgtctttt
     1201 tcctcaacag tccgatattc tttctctcaa gcctgcagat ttaccttaaa aaattcgaaa
     1261 attgtactat gaggaattta gaattctgaa ctgttgtact gtaaaaagcc gtacaaaatc
     1321 agattctgaa tgagagggaa tatgtcggaa aaaattctga aattatcagc tattattgta
     1381 ttattgaata tgtcgcatct ttctgcagag gatgtcggtc cgagccctgg aactcctcag
     1441 gctccggcag aagaaaagga accgggggac ctattttatt ataaaagtga atacactgat
     1501 ctctggatta aaaagagaac tctggaaaaa gaggtggctg agcttaaggc aaagcagaat
     1561 gcagcagata agaggtcgat ggatgagaat gccgcgaata agacaaggct ttctgaactg
     1621 gaaacagaac tggctgcagt gaatcagaag tattcagaac tctatgacaa gtaccagact
     1681 tatgtaagag aaaatgaagc aaagctttcc agtatggaca acagtattgt ttccttaaga
     1741 tcccagaaag aggctgctga aagaagatac ttggcagtca aggaagaaaa ggaagttctg
     1801 gaaagagaac ttctggcaat ccggaatcag aaggatggtc tggacaaaaa ttctctggct
     1861 ctgaaggagc agaatgcact tcttgagaaa aagctgaaag aacagaatct tcttctggag
     1921 cagaagaaca gggaactggc aaaggcggaa aaaacagcca gggaatacaa tgaatccatc
     1981 ctggagaagg attccatttc gcaggaacag gcaatcatct acagggatac aatctccaag
     2041 atagaacagg agaaaacctc ccttcaggaa aaaaacaaca gactgactga agaagtggaa
     2101 agactgaagg agatcaacag ggcatatctg cagaggctgg aagaagcaaa tgcaagactg
     2161 caggaactgg aaacttccaa aagagctctt cagaaaaaac ttgaggattc caaatccgat
     2221 gaggcaatgc tcaattccct cagaaaggaa aatgcagaaa tcagttatca gagagataca
     2281 cttctgaaag aacttgatga tatgaggaag agtgtgactg acagaagcgc ggaactggac
     2341 agagaactca gcagccttca gaaggattac aatgcaagag aaaagcagct ggaggcagaa
     2401 ctggaaagac tgagacagag agagactgaa cttctggcag agaattcgag acttattgct
     2461 gcaaatgatg aaattgcata cgaaaatctc agactgaagg aacagctcga atccatcaac
     2521 cgtgccagaa aggaaatgga aaagtctttc gagaatgaaa tccgcacggg tgaactcgat
     2581 gtcaatcaga acaagaaagg cgttgtgata ggaattcctc tgaatgtcac ttttgacacc
     2641 tattccagca gaatcagacc ggaaggcagg aagatactcg acaagatcat caggatactc
     2701 aatacatata aaataaaaaa aattactatc gagggaaata cggacgacag acctgttctg
     2761 aaaacttcca agtacaggga caactgggag ctttcgacag caagggcgat cagtgttctc
     2821 agatacctcg tccggtacag cgacattcat cctcgattta tgacggctgc aggaaatgca
     2881 gagtacaatc ctctcaagtc caacaacagt ccaagaaacc gggctgcgaa cagaagagtg
     2941 gaaatagtgg tcactcctgc agtgacaaag taggattctc atggaagaag aaaaaggact
     3001 gttttcagaa ttcttcaaag tgcttgcaaa tctggatata ggagaggagc aggcatccat
     3061 tctctgggaa gaggttctga agaatgcaca gaggctttct gaaaaggtcg gccgaaaggt
     3121 cggtttcagg gttgcacttc tggactatat gctgaacgaa aagcagatca tgaagaatcc
     3181 tacttttgtg gagcaggata tttttgaaga agtgatgaga aattcctttg tggatgaact
     3241 gaccgggatc tacaacagaa gatatttcaa tactgcattt cacagggagt tcaaaaggag
     3301 ttccaggtat gagaagacta tctccctttt ctttttcgat gtagacaatt ttaaaagttt
     3361 taatgacaat tacggacatc tcgaaggaga cagggttctt cagctgattg caaagacgct
     3421 ctctcagctg ttccgtgcag aggacattgt ggcaaggatt ggaggggagg agtttgctgt
     3481 tcttcttccg gaaactggaa ttgagtctgc ccgcactgcc tgtgagcgtt ttgcacagaa
     3541 gctgaagata tcttcctctg aaataatgaa aagggagatc actgtcagcg gaggaattgc
     3601 tgcttttccg cattctgcat ctacaatgga tgatctttat aaaaaggcgg atgaactgtc
     3661 ctacaaggcc aaaagaagcg gcaaggacag gatcgttact gaataagaat gccggatgaa
     3721 aaacaggttc tgcataagtg tttttcttta tgcaagagag ggcagtgaaa atatatttct
     3781 tgaatacgaa aatgcagtaa tgccgctttt gaaagaacat acgggtgagc ttcttctccg
     3841 aatcagacct gaccgatctt ctgaagcgga ttcaaaaatc agagttccga atgaaattca
     3901 tctgattggt ttttcagaag agaaaaattt cagaagtttt ctttctgacc cagtgcgaaa
     3961 atcactcgaa catatgcgga aggaaagcat tgtgttttcg attgtcctgc agaacgctga
     4021 gacctgcatt cctgaatcat tttatctgaa taatggttga cagagagatt tttcagcagt
     4081 atcctgaaac tgtattcaga tatgaaagac atactcttta agtacggcag tatattggaa
     4141 aaaatgaata tccgatgcgg atattcattt ttaaatcaga tattgaaaca gtttctcggt
     4201 cggccgtctg ctccgttttt ttccggaaaa aatattttat atttattttt atttatattt
     4261 atctctgcgg gttgcaaaaa gaacaaggct gaaagagtcg agggagacag agagtccgga
     4321 tcggccatta ccatcaggaa ctatcagaga atttcagtga acgacaaggg tgaaatgctc
     4381 tggaagctca aggccgaaga aacctatttt ttcaaggaag acaacaggac aattctttac
     4441 aagattgaag ccgatcagct tgaaaaggga aagtcgaagt ccagaatgaa agccgacaag
     4501 ggatatctgg acagaaagaa tgaaatactg aaagtatccg gaaatattta catcaggaca
     4561 aacgacggga aggaactgac cggagaagag atggactata atatagcaaa ggaacttctc
     4621 acttccgaca aaagggtagt gatcagaaca gggaaaacgg tgattcgcgg aagaggactg
     4681 gaggcagaca acagtctgaa caagttcaag atcatccatc cggaaggtgt cactgtggga
     4741 acggacaatc cattcaagga caactgagat gaaaagactt tttgcacctg ttctctgcat
     4801 cttatttatt catacagcca ggcccccact tctgatgccg tacttcggaa aggctcctgt
     4861 actggacgga actgcatc
//
LOCUS       DAOQGJ010000051         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9102_length_4868_cov_6.014544, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000051 DAOQGJ010000000
VERSION     DAOQGJ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9102_length_4868_cov_6.014544"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..3252)
                     /locus_tag="PK683_01415"
     CDS             complement(1..3252)
                     /locus_tag="PK683_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07130.1"
                     /translation="MKRLFVLFLISFFSGGGTAWPLVSALAFGKNQKKSPFYPLIPQS
                     VAAETPNTSVSAPAGFTVSKREGLMTSENGTTDSFTVVLNKKPTANVILNILSSDTQE
                     ITVTPSELVFTPDDWSAAKQVVLTGINDGPAIDGNKSVRIILGQGISSDSEYNSLSAG
                     EVSATNVDNDSAGIAVSQMSNLNTSESGDSDTFTVALSSAPTANVVLTLVSNNTAEGT
                     VSPTTLTFTPSNYSTPQTVTVTGADDGTADGNIPYTVSITNAASSDLAYNGLSVPNIS
                     VTNFDNDTPWVFIDDTVLTTYEQPGTAKTYRVKLLKAPTANVTVSMVSNDTTEGTVSP
                     ASLTFTPGNYSSFQTVTVTPVYESAIDGDITYTVRNTPSSADLNYNALLPVDVTVTNV
                     DAVPGFEISAVSGATDEFAATATFQIRLKAPPSSDVTLNLTSSNPLEGVIQSGSSLTF
                     TSANWSIFQTVIVRGVDDSIKDPNKGYTIITSPAVSADSAYSGMNPSDIDLVNGDNDT
                     PGYTVSTTSVSQNEGGTQTFTIRLASRPTANVTVNLSSANTSIATVSPASITFTALSW
                     STMQTVTVTMVDNFADTANNGTSILISNAVSTDPNYSGLFSTAVTATSVDNDTRGVTL
                     ANNTSKFMFDSLSSTESFTVKLNSQPTADVIYDVTSLDTSKLTASPSTLTFTPANWNT
                     AQTVTLTGAGNPDINAADANVTVRVGDALNRPQSGLSDYNLTTAGNVSVNVCDLDGGY
                     RVAVCRPSGAFTTVFNENGGTSSQFILGISDVPTANVTTTITSSDTTECNNMGTVTFT
                     PASRISGKLSVSAFNDVILDGNQTCTMTASNTASTDNNFNNLAVTLPATLFTVTDNEV
                     PNINSSPNNLSTGARLTVRTSSVNTFNVTLTFAPAANVTYNLSVGAGCTGSSVSPASM
                     TFTSANWNSPQTVTFTAGTNSGNCQIITGNFSSADPVWNGTNPSDFYFNVQSPGVTVT
                     LPTGSAPYLTSEIGTTQTFQVRLNSAPSSNVTIAVSSSNTAEGTVSVSSLTFTPANWS
                     TNQTVTVTGADESVFDGDQNYQIFLSNTSSADAGYNGVSFSNQTINFRNIDND"
     gene            3579..4832
                     /locus_tag="PK683_01420"
     CDS             3579..4832
                     /locus_tag="PK683_01420"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNH07131.1"
                     /translation="MENKNRKAGINQPENISLPFKIEECLAKGLMLELIMRLGKESLD
                     EMIEDDIEKLCGARYVHNPARENSRWSRTKSEVTLGGCKAGIEHSRVRNVRSREEVKI
                     PVLKTLKNLDLLQERHAGQMILGVSTRKVKRSLEGQTAFKSYGTSKSAVSRNFVSLTR
                     KKLADWLGRNLDTEYPFIMIDGISFRGKTVLIALGISKDGHKSVLGAWEGSTENARVC
                     TDLLQNLIERGLHQESVKLLIMDGGKGIHKAVRDVFGNNILIQRCQIHKKRNVKDYLP
                     ENMKTSVESIMNDAYSGTDYARSKKMLENLAASLRQKGQEPAAASLMEGLEETLTLLR
                     LGAPEEIQKTLSNTNAIENLNGSIRRMTQRVKKWSGNNMLMRWISSALIESEGNFRRV
                     KGYRSIPVLLDRISQLNNFVLPEVA"
BASE COUNT         1350 a          977 c         1191 g         1350 t
ORIGIN      
        1 tcagtcattg tctatattcc tgaaattgat tgtctgattt gagaaagaca ctccgttgta
       61 gcccgcatct gcactggatg tattggaaag aaaaatctga tagttctgat caccgtcaaa
      121 tacagactca tctgccccgg tcactgtcac agtctgattt gtgctccagt tggcaggagt
      181 aaatgtaaga gaggatacag aaactgttcc ttcagctgta ttgctgctgg ataccgcaat
      241 ggtcacattg gaagaaggtg ctgaattgag tctgacctga aatgtctgag tagttcctat
      301 ttcagaagtg agatacggtg cagaacctgt cggaagagtc actgttacac ccggagactg
      361 aacattgaaa taaaagtctg acggattggt tccgttccag acaggatcgg cactgctgaa
      421 gtttcctgta atgatctggc agtttccact gtttgttcct gctgtaaacg taactgtctg
      481 aggagaattc cagtttgctg aagtgaacgt catactggca ggacttactg aagagccagt
      541 gcagcccgct cctacgctta aattatatgt gacatttgca gccggagcaa acgtaagtgt
      601 aacattgaat gtgtttacag aagaagttct gactgtcagt cttgcacctg tactcagatt
      661 gttcggagaa gagtttatat ttggaacctc attgtcagta actgtaaaca gagttgcagg
      721 cagtgtgaca gcgagattat tgaaattatt gtctgtactg gctgtattgg atgcagtcat
      781 tgtgcaagtc tggttcccgt ccagaataac atcattaaaa gcggaaacac tcagcttgcc
      841 tgaaatcctt gatgcaggag taaaagtgac tgttcccata ttattgcatt cggtagtgtc
      901 agaagacgtt atagtggttg tgacgttggc tgtcggaacg tccgaaattc ccagtataaa
      961 ctgtgaactt gttcctccgt tttcattgaa tactgttgta aatgcaccgg aaggcctgca
     1021 cacagcaact ctgtagcctc cgtccaggtc gcatacgttt acgctgacat ttccagctgt
     1081 ggtcaggttg taatcgctca gtcctgactg aggtctgttc agagcatctc ctactcttac
     1141 tgtcacattg gcatcagccg cattgatatc gggatttcct gcacctgtga gagtaactgt
     1201 ctgcgcagtg ttccagtttg caggagtgaa agtgagcgtg gacgggcttg cagtcagttt
     1261 agaagtatcc aggcttgtta catcgtatat aacatcggct gtaggctgag aattcagttt
     1321 tacagtgaat gactcagtgg aactcagaga gtcaaacata aacttcgatg tattgtttgc
     1381 aagagtgact cctctcgtgt cattgtctac tgaagttgct gttactgcag tgctgaaaag
     1441 tccgctgtag tttggatcgg tgctgactgc attggaaatg agaatgcttg tcccgttatt
     1501 ggcagtgtct gcaaaattat ccaccatagt tacagtaact gtctgcattg ttgaccagct
     1561 cagtgccgta aatgtaatgg aagcaggaga cactgtcgca attgaagtgt ttgcagaact
     1621 taaatttaca gtcacgtttg cggtcggtct tgaagcgagc cggattgtaa atgtctgagt
     1681 tcctccttca ttctgggaaa cacttgttgt gctgacagta taacctggag tatcattgtc
     1741 gccatttacc aaatcaatat ccgaagggtt cattccgctg taagcagagt ctgcactgac
     1801 tgcgggagat gtaatgattg tatatccctt gttcggatcc ttgatggaat catcgacgcc
     1861 tcttacaatg actgtctgaa aaatagacca gtttgctgaa gtaaacgtca gagaagaacc
     1921 tgactgaatc acaccttcaa gaggattgct ggaagttaaa ttgagtgtta catcagatga
     1981 aggaggtgcc ttgaggcgta tctgaaaagt cgctgtggcc gcaaattcat ctgttgctcc
     2041 gctgactgca ctgatttcaa atccgggaac agcatccaca ttcgtaactg ttacatccac
     2101 cggaagaaga gcattataat tcaaatccgc actggaagga gtatttctga ctgtataagt
     2161 gatgtctccg tcaattgcag attcataaac aggggtgaca gtcacagtct gaaaactgga
     2221 atagtttccc ggagtgaatg taagagatgc cggactgact gttccttcag ttgtatcatt
     2281 gctcaccata ctgacagtca catttgctgt cggcgccttc agcagcttga ccctgtatgt
     2341 tttggcagtg ccaggctgtt catatgtagt cagaactgta tcatcgatga acacccaagg
     2401 agtgtcattg tcaaaatttg taacactgat attggggaca ctcaggccgt tgtaagccag
     2461 atccgaactt gctgcatttg taatggaaac ggtatatgga atatttccgt cagcagttcc
     2521 gtcatccgct ccggtcactg tcactgtctg aggagtggag tagtttgacg gagtaaatgt
     2581 aagtgtggtc ggactgacag ttccttctgc agtattattg ctgacaagag ttagaactac
     2641 attggctgtg ggtgctgaac ttaatgctac tgtaaatgtg tcagaatcac ctgactcaga
     2701 ggtgttcaga ttgctcattt gggaaactgc gattcctgcg gaatcattgt ccacatttgt
     2761 cgcggacact tcacctgcac tgaggctgtt gtattcagaa tcagaactga ttccctgtcc
     2821 aaggataatc cttactgatt tgtttccgtc gattgccggt ccgtcattga ttccagtgag
     2881 aactacctgc tttgccgcag accagtcatc cggagtaaat acaagttctg aaggagtaac
     2941 tgtaatttcc tgtgtatcac tggataaaat atttaatatt acatttgcag tcggtttttt
     3001 attcaaaacc acagtaaaac tgtctgttgt tccgttttcg gatgtcatca gtccttctct
     3061 tttgctgacg gtgaagcctg ctggagcaga tactgaagta ttgggagtct ctgcagcgac
     3121 agactgtgga attaaaggat aaaacggaga tttcttctga ttcttcccga acgcaagtgc
     3181 tgaaaccaac ggccaggcag tgcccccgcc ggagaaaaaa gaaatcagga ataatacaaa
     3241 taaccgcttc atattatata tgacgaaata attcggtaag tacacatagc tttcgttttt
     3301 tattttctca gaaagagatt ggaattgaaa ctgagtttca attatatgaa ttcgattgta
     3361 cattaggacg gtttgtttcc ttcattcagt atctgctgaa atccggatat ttgcggagaa
     3421 tgcttccata cgactatctt ttgaaccttg taaataatga ttttgagttg ccaaagaggc
     3481 aatgtcccat tgaatgttga aattaaatga gtggtgatct caatactgat tttaaaatta
     3541 gtgttgcgaa caaaaattaa agaggagaaa caccacccat ggaaaacaaa aacaggaaag
     3601 ccggaataaa tcaaccggaa aatatttcac ttccctttaa aatagaggaa tgtctggcaa
     3661 aaggtctgat gcttgaactg atcatgaggc tcggcaaaga atctctggat gagatgatcg
     3721 aagacgacat tgagaaactg tgcggagcga gatacgttca caatcctgcc agagagaaca
     3781 gccgctggag caggacaaaa tccgaggtga cgctcggagg ctgcaaagca ggcatagagc
     3841 attcacgggt caggaatgta aggagccgtg aagaggtgaa gattccggtg ctgaagacgc
     3901 tgaagaatct ggatctgctg caggagcgtc atgccgggca gatgatccta ggagtctcga
     3961 cccgcaaggt gaagcgttcg cttgagggtc agacagcgtt caaatcgtat gggacatcaa
     4021 agagtgcggt gagcagaaat tttgtctcac ttacacggaa gaaacttgcg gactggctcg
     4081 gaagaaatct ggatactgaa tatcctttta taatgataga cgggatttcc ttccggggaa
     4141 agactgtcct gattgctctg ggaatcagta aagacgggca taagagcgtt ctcggagcct
     4201 gggagggcag caccgaaaat gcaagggtgt gcactgatct tcttcagaat ctcattgaaa
     4261 gaggtctgca tcaggagtcg gtgaagctgc tcatcatgga cggcggaaag ggaattcaca
     4321 aggcagtcag agacgtgttt ggaaataata tattgattca gagatgccag attcacaaga
     4381 aacggaatgt caaggattat cttcctgaga acatgaagac gtcggtcgag tcgattatga
     4441 atgatgccta ttcgggaact gattatgcac ggtcaaagaa gatgcttgag aatcttgccg
     4501 cttcactgag acagaaagga caggaacctg cagcggcttc tcttatggaa ggccttgagg
     4561 agactctgac tcttctgaga ctcggtgcgc cagaagaaat tcagaagact ctttccaata
     4621 ccaatgcaat cgaaaatctt aacggaagca tacgccggat gactcagcgt gtgaagaagt
     4681 ggtctggaaa caatatgctt atgcgatgga tttcttcagc tcttatcgaa agtgagggaa
     4741 atttccgacg agtcaaagga tacaggtcaa ttcctgttct gcttgacaga atatcccaat
     4801 tgaataactt tgtattacct gaagttgctt agtatctgct catttacaac aatgttcggg
     4861 acattgcc
//
LOCUS       DAOQGJ010000052         4835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9196_length_4835_cov_3.400000, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000052 DAOQGJ010000000
VERSION     DAOQGJ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9196_length_4835_cov_3.400000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..3091
                     /locus_tag="PK683_01425"
     CDS             128..3091
                     /locus_tag="PK683_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07132.1"
                     /translation="MVEFFLFVSYIVLIVLWRRNSALKEDLHKSNLRMASMEKKLNDL
                     QSLPVHSAEIYVQEKPAEISKPLTESEEKKEEIPSAAYTAKEEKITLDSTVISDEKME
                     IRASEEIQDAVTEKKERSWWQKTEKSLLENWTGILGAVILVLGVGFLAAVAFIIVPPF
                     VRFLIISGFAGSVYWISGYIKRKDESWEKLSDILFSTSAVIFLIACIGSYGISGIKFI
                     EDPLFSIPFILLGLGVNLCAGYSRNKETYASLHIALTFIGLASAPQNLVILFIASAVT
                     LLAVRKNYAVRWDWHLFLTAFSYFCFHIYNYSERESAVKSDAFLRISGIILVSSVYIN
                     ALYVHYIEAFYSEEKLPRTALLTHISSWIFLGISLLAYSTGSQWTPIYILAVGAGAFV
                     LSQTAKRRNIRWLYVCDMWAAEALILIGIFGLEKWDIPIFVLILLSGVQAMIFALWNI
                     HEKSPILAGSSVLLLSVFQGAMAVIVILNQERAANLDFRDILNLSAFTVFVFLEGIYY
                     HYSKKIGTDFLNENRILYWISEIPGFIFAVSFLSLYAYIDRQIYAPAAFAALICGIGF
                     YRNRIQSPGIGAGLLFLFIISLTDSWNDMKSFGSEKPLDFLSETAPHFFSIFFLMKFS
                     YIREFDRKFSIPFLYIFNTHLLLSIHFFTAGVSPFLTGVSALLISLILTEFSENSSLT
                     GFIEDSGSSKHLIINSLIFSAVFLYRHLLIHLQSGAYLWIFPVRFWIEIFALSAFIFI
                     LFRKSSIYSFYNENIQPFFAELSLLFFVLAGLTVLPEHWSPPYWGALALVCYLISNVK
                     IMDLSRFAQYSLIIQAVNCFYIAFISSSEDNPGGLWQNSKWIPALTAIALTFPYLYLL
                     YGSFRQKEIKYPGYLSKADRVSVLAQNRVFSLAFYPFFAAVFLFLYWSASGAILTLLW
                     VVFAFAVFAFSLLVKEKHFRYASLGILFFCIFRLIFHDLSSTGALTRAFVFVGTGIIL
                     LIMNSLYNKYKDRLE"
     gene            3088..3984
                     /locus_tag="PK683_01430"
     CDS             3088..3984
                     /locus_tag="PK683_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNH07133.1"
                     /translation="MKNGRLYFVLILCFGIGLIAALSVQEKKTEMDSTLAPFWQLIGK
                     PVQLGSKALTKVIPVSALDEKEYGDAIRSRYESGADKSDPRYIYLNTLIKDLTKTKSK
                     PFDYTVFLMDSGVPNAFAMPGGVLFVTTGLLQILESESELVSILGHEIGHVEKNHCLE
                     NVRFQLLTQKIGSETLGKLADLAFGIIVKSSYNKNQEEEADEYAFSLLLSSDYHPAGE
                     GKAFEKLAESHLGKETRSANIITEYFQSHPHTVLRKERYQEKAKAWRASNSGVRKYTG
                     KANLELQKTYSENQIENEWSAE"
     gene            complement(3987..4403)
                     /locus_tag="PK683_01435"
     CDS             complement(3987..4403)
                     /locus_tag="PK683_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07134.1"
                     /translation="MEKLYEKEGVAAIYWDAASQALCPEWKSFSVSDKEVKNVLENLL
                     IFIAQKNCDRQIINTGKTLGGFSEEIQEWLGNDWIPRAVKAGMKYVATVTPKNAMAQL
                     SNDYWQEVSTDIGFVTINVTSHEDGQEWLKKFPLKT"
BASE COUNT         1317 a         1048 c          992 g         1478 t
ORIGIN      
        1 aggcattatt gatccctgcg gaaattactg gtggatatcc aaaagactca caaagaaagg
       61 ttatcatgaa taagggacaa aaaatttact tgtaattcct ttccttcccg atttactata
      121 cctcgctatg gtagaattct ttctgtttgt ttcctatatt gtactgattg ttctctggag
      181 aagaaattcc gcactgaagg aggatctgca taaatcaaac ctgagaatgg cttcgatgga
      241 aaaaaaactg aatgaccttc agtctctccc ggtccattcc gcagaaatct atgttcagga
      301 aaaaccagcc gagatatcga aacctctcac tgaatctgaa gagaagaaag aagaaatacc
      361 gtctgctgca tatactgcaa aagaagaaaa aatcacactg gacagcacag tcatatccga
      421 tgaaaaaatg gaaatacggg cttctgaaga gattcaagat gctgtcactg aaaaaaaaga
      481 aagaagctgg tggcagaaaa cggaaaaatc tcttctggaa aactggacag ggatacttgg
      541 agcagtcatc cttgttctcg gtgtaggatt tctggccgcc gtggcgttca tcattgttcc
      601 cccattcgta aggtttttga ttatcagtgg attcgcaggt tcagtatact ggatttcggg
      661 atatattaaa aggaaggatg agtcctggga aaaactttcc gatatccttt tcagcacttc
      721 cgcagtcatt ttcctgatcg cctgcatcgg cagttacgga atttcgggaa tcaagtttat
      781 tgaggaccct cttttttcca tcccgtttat tctgcttgga ctcggagtaa acctgtgtgc
      841 agggtattcc aggaacaagg aaacgtatgc cagccttcat atcgctctca cattcatcgg
      901 actggcttcc gctccgcaga atcttgtcat tctgttcata gcttccgcag tgacattact
      961 tgctgtccgc aaaaattatg ctgtccgatg ggactggcat ctttttctca ctgcattttc
     1021 ctatttctgc tttcacatct acaattattc ggagagagaa tccgcagtga agtctgatgc
     1081 atttctcaga atctccggaa tcattctggt cagctctgtg tatataaacg cattatatgt
     1141 tcattacata gaagcatttt actcggagga gaaacttccg cgcacagctc ttctcactca
     1201 tatcagttcc tggatttttc tgggaatcag tctgctggca tattccacag ggagtcaatg
     1261 gacgccgatt tacattcttg cagtgggagc gggggctttt gttctctctc agacagcaaa
     1321 aagaagaaat atcagatggc tttatgtatg tgatatgtgg gctgccgagg cactgattct
     1381 tatagggatt ttcggactgg aaaaatggga tattccgatc tttgtgctga ttcttctttc
     1441 aggagtgcag gcaatgattt ttgctctctg gaacatacat gaaaaatctc cgatacttgc
     1501 aggatcatct gttcttttat tatctgtgtt tcaaggagca atggctgtaa tagtaatcct
     1561 gaaccaggaa cgagccgcaa atctggattt cagggacatt ctgaatcttt ccgcattcac
     1621 tgtatttgtt ttcctcgaag ggatatacta tcattattca aaaaaaatcg gaactgattt
     1681 tttaaatgaa aacagaatcc tgtactggat ttcagaaatt cccggattta tctttgcagt
     1741 atcgttcctg tcgctctatg cttacataga cagacagatt tacgcaccgg cggctttcgc
     1801 agcactcatt tgcggaattg gtttttacag aaacagaatc cagagtcccg gtattggggc
     1861 agggctcttg tttctgttta tcatctcact gaccgattca tggaatgata tgaagtcctt
     1921 tggatcggaa aaacctctgg attttctttc agaaacggct cctcattttt tcagcatatt
     1981 ttttctgatg aagttttctt atatcaggga attcgacaga aagttttcca ttccgtttct
     2041 ctatatattc aacacacatc tacttctcag tattcatttt ttcactgcag gtgtttcccc
     2101 ttttcttacc ggtgtttctg cccttctgat ttcacttata ctgactgaat tttctgagaa
     2161 ctcttccctg actggcttta ttgaagattc cggttcttca aaacatctga taataaacag
     2221 tctgatattc agtgcagttt ttctttacag acatctcctc atccatctgc agtcaggtgc
     2281 gtatctttgg attttcccag tgcgtttctg gatagagatt ttcgcacttt ctgcattcat
     2341 ttttattctt ttcagaaaaa gctccatata ttcattttat aatgaaaata tacagccttt
     2401 ttttgctgag ctgtccctgc tgttctttgt gctggcagga ctgacagttc ttccggaaca
     2461 ctggagtcct ccttactggg gtgctcttgc tcttgtctgt tatcttattt caaatgtgaa
     2521 gattatggat ctgagcaggt ttgctcagta ttctctcatc attcaggcag tgaactgttt
     2581 ttacatcgca ttcatttctt cgagtgagga caaccctgga gggctttggc agaacagtaa
     2641 atggatacct gcattgacgg caatcgcact tacttttccc tatctgtatc tgctgtacgg
     2701 cagtttccgg cagaaggaaa tcaaatatcc aggatatctt tccaaagcag acagagtatc
     2761 cgttctcgca cagaacagag ttttctcgct tgcattctat cctttttttg ctgcagtctt
     2821 tctgttctta tactggtctg catcaggagc gattctcaca cttctatggg tcgtatttgc
     2881 atttgcagtc ttcgcgttca gtctgcttgt aaaggaaaaa catttccgtt atgcaagtct
     2941 gggaatactt ttcttctgca tattccgtct gatatttcac gacctttcat cgacaggagc
     3001 actgacaaga gcatttgtat ttgtaggaac cggaatcata ctcttgataa tgaacagttt
     3061 atataataaa tataaggaca gactggaatg aaaaacggca gactgtattt tgttttaatt
     3121 ttatgcttcg ggataggact gattgctgca ctgtcagttc aggaaaagaa aacagaaatg
     3181 gactccactc tggctccgtt ctggcagctg ataggaaaac ctgtccagct cggaagcaag
     3241 gctctcacca aggtaattcc tgtcagtgca ctggacgaaa aggaatacgg agatgcaatt
     3301 cggagcaggt acgaatcagg tgcagacaaa tctgatccca gatacattta tctgaacact
     3361 ttgataaagg atcttactaa aacgaaaagc aaaccttttg actatactgt attcctgatg
     3421 gattcaggag tgccgaatgc atttgcgatg ccgggaggag tactttttgt cacgactgga
     3481 ctccttcaaa tactggaatc agaatcggaa cttgtttcca ttctaggcca tgaaatcgga
     3541 catgtggaaa aaaaccactg tctggaaaat gtgcggtttc agcttctgac ccagaagatc
     3601 ggatcagaga ccctgggaaa actggcagat cttgcattcg gcattattgt taagtcttct
     3661 tataataaga atcaggagga agaggcggat gaatatgcat tcagccttct tctctcatcg
     3721 gactatcatc cagcaggaga aggaaaagca tttgaaaagc tggcagagtc tcatcttgga
     3781 aaagagacca gatctgcaaa tatcattaca gaatatttcc agagtcaccc ccatacggtt
     3841 ttgagaaaag aaagatacca ggaaaaagcc aaggcctgga gggcatccaa ctcaggtgtc
     3901 cggaaatata cagggaaggc caacctggaa ctgcagaaaa cctattcaga aaaccagata
     3961 gaaaatgaat ggagtgccga atgaattcag gtcttaagag gaaacttctt cagccattcc
     4021 tggccgtcct cgtgtgaggt cacattgatt gtgacaaatc caatgtcggt gctcacttcc
     4081 tgccagtagt cattggaaag ctgtgccatt gcattcttag gtgtgacagt ggcaacgtat
     4141 ttcattcctg cctttactgc acgcggaatc cagtcattgc cgagccattc ctgaatctcc
     4201 tcactgaaac cgcctaaagt ctttcctgtg ttgataatct gtctgtcaca gtttttctgt
     4261 gcaatgaaaa tcaataagtt ttcgagcaca tttttgactt ccttgtctga taccgaaaaa
     4321 cttttccatt cgggacataa agcctgagat gcagcatccc aataaattgc tgccactcct
     4381 tctttttcgt acaatttctc catacaatca gcctcctgcc tgtttaatga gacaatgccg
     4441 aaatcactct tcttaagcaa agctaaaaaa gagtaagaca aacctaatat aaatcacttt
     4501 atggatcatt tcgagactga taatttcaat ggtgttcagt tttgcagatt caaagccaaa
     4561 ctctcccctt ccttcgtcat atgctatccg ctgcttcatt tcttccgctt aaaattccag
     4621 tctttaaacc aaccaatcct tacagtcaga ctattcccgg atttccaatc ttgcaagctg
     4681 ggaaaatgcc agcttgttcc gcccttcagg ttattagaaa tcaaaagaga aaggagacct
     4741 gccgtatttc ctagcttgtg ctggtttagc aagcatacag ccggacgggg gtttaagcgg
     4801 ccgcttcgcc aagttattca agacggctgc tcata
//
LOCUS       DAOQGJ010000053         4829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9216_length_4829_cov_3.554252, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000053 DAOQGJ010000000
VERSION     DAOQGJ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9216_length_4829_cov_3.554252"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PK683_01440"
     CDS             complement(<1..772)
                     /locus_tag="PK683_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH07135.1"
                     /translation="MSTLKYFIIYILAAAAFGCTEQKKEDNFVLTEEWEYKTGFDPAY
                     LDEDGSGWKKISLPENLTKKLKDEQPFNSVTLRKKIPVEIAQIAESGSGIALNAGRVL
                     DVSIYYMNGKYFGQSGSIDPYKTAAMRPFLREIPAGLIRPKDNYLQIVLYTNGDFPLQ
                     FMDELKIGRADLVFTDRNSSEIIAFIFLSVYMSAGIYHLLLFRKRSQDRYNLYFGLFC
                     IVVSLYWFTSNTVTRDIFFHDRVFLHRKMEHIFLFFTSP"
     gene            complement(884..3079)
                     /locus_tag="PK683_01445"
     CDS             complement(884..3079)
                     /locus_tag="PK683_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07136.1"
                     /translation="MLQFLPSEGKLLLNGQRMLIFSQNSFASLHRLLVHHFGFEYSNA
                     LSSQFGWQCGTDDYVSIAEKGDWDTEEDKITSGPVIHMWEGIVHVEPLKIEYDRRAGK
                     FYMTGIWKNSYEAENYINTYGKSQNPVCFTLTGYASGWASKFFGKELIAVETSCIAKG
                     DPCCSFEIRPIEEWGNEALPWIKALKATSSSISSFLEKEVKEKTEDLQRLNEELERAA
                     VFAVEGAIAKSNFLASMSHEIRTPMNGVIGMAELLSHTSLNDEQRDFVESLLSSSELL
                     LSIINDILDYSKLEAGRMELYETDTEIRDCISECVMMFQYKLKEKRQELTKFISLDVP
                     GFLLIDEKRLKQVIINLLSNAVKFTPMNGEIHFELLVKQDSEDHQYLLFTVKDTGIGI
                     PEEKQKNLFQSFSQVDASTTKNFGGTGLGLAISKKIVEQMRGKIWVQSSPGKGSVFYF
                     EIPLKRSAVQDPKYLSSSDPALNGKRAAVIDDNQVNLKIFQSLLNHWNMKTDCFSSPL
                     LALGSLETLNADIFLLDFNMPEMDGLTLGRKIREHYGKSVHIALCSSTDLDEMSLRFD
                     PSIFDFILSKPIKQKVLFERLLRLYNQDYNQSVISDVRYDPEKTRTAKILLVEDNPIN
                     QKLAVKMLRKLGFSADIADNGKSAVEKALSTDYDLIFMDIHMPEMDGEQAAAIIQASL
                     QNPPKIVIMSADAFRKEEIRMTSIRIEEYILKPIKISDLESILTRNHLL"
     gene            3384..4328
                     /locus_tag="PK683_01450"
     CDS             3384..4328
                     /locus_tag="PK683_01450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH07137.1"
                     /translation="MTNELNLLLSVMDTIPDLICLKDTEGRYILCNKAFERYFGASKA
                     QLSGRTDFDFKTTETAEAQRKQDSEILEKGESSIFYEWITFSESGETSLVETIKTPMK
                     DESGRLIGILSISKDVTEQKVMEEKLREVNLQLLYLSDKDALTGLANRRKFNDHLDME
                     WHHAVRRKESIALIMLDIDKFKQYNDAYGHLAGDQCLYKIGTLMSNIIKRHTDLAARY
                     GGEEFGIVLGNIDEHHTFEMAERVRKGIFELNILHSASSYFNSRNEPVVTVSIGAAVV
                     IPKRNSEPEELILLADQALYKAKADGRNRTVLYTPENS"
     gene            4399..>4829
                     /locus_tag="PK683_01455"
     CDS             4399..>4829
                     /locus_tag="PK683_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07138.1"
                     /translation="MPERPYIYYDFTLSLCPVCLKRIDAKIVMQDSGVYMMKTCSEHG
                     SFKVLIEEDEVYYRNIRNYAKRSEMPLHFGTEVIHRCPMDCGLCSDHEQHSCLTVLEI
                     TDRCNLECPTCYSASSPRAGRHRTLSEIEFMLDRIVYNEGRP"
BASE COUNT         1484 a          996 c          938 g         1411 t
ORIGIN      
        1 gcggagatgt aaagaacaga aaaatatgct ccatttttct gtgcagaaat actctgtcat
       61 gaaagaaaat atcccgagtc actgtgtttg aagtgaacca gtacaatgat actacaatac
      121 agaacagtcc aaaatacaga ttatatctgt cctgagaacg tttacgaaaa agcagcagat
      181 gatatatccc tgccgacata tatacggaaa gaaaaataaa tgcaatgatt tcagaactgt
      241 ttctgtcagt aaagaccaga tctgctcttc ctattttcag ttcatccata aactgcagag
      301 gaaaatctcc gtttgtgtaa aggacaatct gaagataatt gtctttggga cgaatcagtc
      361 ctgcaggaat ttcgcgaaga aaaggacgca ttgctgctgt tttataggga tctattgaac
      421 ccgactgtcc gaaatatttt ccattcatat aatagatgga gacatccaga acccttccgg
      481 cattcagagc aattccgctt ccgctttccg ctatctgcgc tatttcaact ggaatttttt
      541 ttctcaaagt aactgaattg aacggctgct catccttgag cttctttgta agattctccg
      601 gcagagatat tttcttccat ccggatccgt cttcatcaag ataagcggga tcaaatccag
      661 tcttgtattc ccattcttca gtgagaacaa aattgtcctc ttttttctgt tcagtgcatc
      721 cgaatgcagc ggccgcaagg atatatataa taaaatactt taaagtactc atacagaatc
      781 atgtccaaat tcaataaggc tgacaggcag aagtctgttt ttcctgcagg cagaaaaaca
      841 gtaatgagag gatgaaatat tttccatatc gaatactgat aatttataga agatgattcc
      901 ttgtcaatat gctttcaaga tccgaaatct taatcggttt cagaatgtat tcctctattc
      961 ttatgctggt cattctgatt tcttccttcc tgaatgcatc agctgacata atgactattt
     1021 taggcgggtt ctgcagactc gcctgtatta ttgcagctgc ctgctctccg tccatctcag
     1081 gcatatgaat gtccataaag ataagatcat aatcggtact gagagccttc tctactgcgg
     1141 actttccgtt gtctgcaatg tctgcagaaa aaccaagttt tctcagcatt ttgactgcaa
     1201 gtttctgatt gatcggattg tcctcaacaa gaagtatttt tgcagttctt gttttttccg
     1261 ggtcatacct gacgtcagat atgactgact gattataatc ttgattatac agtctgagaa
     1321 gtctttcaaa tagaactttc tgttttatag gcttacttaa tataaaatcg aatatacttg
     1381 gatcaaatcg taaactcatc tcatccaagt ctgtcgatga acatagtgca atatgaacag
     1441 attttccata gtgctctctt atttttcttc caagagtcag tccgtccatt tcaggcatat
     1501 taaaatcaag aagaaaaata tcagcattga gtgtttccaa cgaacctagg gcaagcagag
     1561 gagatgaaaa gcagtccgtc ttcatattcc agtggttcag gagactctga aatattttta
     1621 aattcacctg attgtcgtct atcaccgcag cccgtttgcc gttcagtgcc ggatcagatg
     1681 aactaagata ctttggatcc tgaactgcag atctttttaa gggaatttca aaataaaata
     1741 ctgaaccttt cccaggactg ctttgaaccc atattttccc ccgcatctgc tctacaattt
     1801 ttttagagat cgcaagacca agtccagtcc ctccgaaatt tttggttgta gaagcatcga
     1861 cctgactgaa tgactgaaac agatttttct gcttttcctc aggaattcca atcccggtgt
     1921 ccttgacagt aaaaagcaga tactgatgat cttcgctgtc ctgttttaca agaagctcaa
     1981 aatgaatttc tccgttcata ggagtgaatt tcacagcatt gctcagaaga tttataataa
     2041 cctgcttgag tcttttttca tctatgagaa ggaaacctgg aacatcaagg ctgataaact
     2101 tcgtcagttc ctgtctcttt tccttgagct tgtactgaaa catcatgacg cattcagaaa
     2161 tacagtctct gatttccgta tccgtttcat acagctccat tctgcccgct tctaatttag
     2221 aataatctaa tatatcattg attatactta gcaggagctc tgaacttgaa agaagacttt
     2281 ccacaaaatc tctctgctca tcattgagag aggtgtgaga aagaagctcc gccataccga
     2341 ttacaccgtt cataggtgtt ctgatttcat gactcatact tgccagaaaa tttgattttg
     2401 caatggcccc ttcaactgca aatacagcag ctctttccag ttcctcattg agtctctgaa
     2461 ggtcttctgt tttttctttc acttcctttt ccagaaagga ggaaattgag gatgaagtag
     2521 ccttcagtgc cttgatccaa ggcagagctt cattccccca ttcttcaatc ggtctgattt
     2581 caaaactgca gcagggatct ccttttgcaa tgcaggatgt ctcgactgca atcagctcct
     2641 ttccgaaaaa cttggatgcc cagcctgaag cataacctgt cagagtgaag cagacaggat
     2701 tttgactttt tccataagta tttatataat tttccgcttc atacgaattt ttccagatcc
     2761 cagtcatata aaattttcca gcgcgcctgt catactcaat cttcagaggt tcaacatgaa
     2821 ctatcccctc ccacatatga atgaccggac cggatgtaat tttgtcctct tcagtatccc
     2881 agtctccttt ttctgctatt gaaacatagt catctgttcc gcactgccag ccaaactgtg
     2941 aagacagtgc attagaatat tcaaatccaa aatgatgaac taaaagtctg tgaagagaag
     3001 caaaggaatt ctgggaaaaa atgagcattc tctgaccatt cagaaggagt tttccctcag
     3061 atggaaggaa ctgcagcata gcctggacgt ttatattgtc agatttcata tatatataat
     3121 gataatttca tttaacagaa aagaagtaat ttatctcgcc ttgactgcag taaatgagac
     3181 tgactattga tctgcatact attcaaattt ttgcagaata gattcaatca gtagtatgtg
     3241 acagaaggat cactgaaaat gcaagaactt ttcatttttg tttaaaaatg gatcagtctc
     3301 agggtgggaa aaacgaaatt cagactgtta aaaatcattt gacagtataa attcttgcaa
     3361 aaaaatgttc tcaccgaaga gtcatgacaa atgaactgaa tctgctgctg agtgtcatgg
     3421 atactattcc ggatctgatc tgcctcaagg acacggaagg caggtacatt ctctgtaata
     3481 aggcatttga aagatatttt ggagcatcta aagctcagct ttcaggcagg actgattttg
     3541 atttcaagac aacagaaacc gcagaagccc agagaaaaca ggatagcgaa attctggaaa
     3601 agggagaaag ttccattttc tatgaatgga tcactttttc cgaatcggga gagaccagcc
     3661 tggttgaaac cataaaaact cctatgaaag atgaatcagg aaggctcatt gggatactca
     3721 gtatctcaaa agatgtcact gaacagaagg ttatggaaga gaaactgaga gaagtgaatc
     3781 ttcagcttct gtacctctcc gacaaggatg cactgaccgg ccttgcaaac agaagaaagt
     3841 ttaatgatca tctggatatg gaatggcatc atgcagtcag aagaaaggaa agcattgcac
     3901 tgattatgct cgacattgat aaattcaaac agtacaatga cgcttacgga cacctggccg
     3961 gtgatcagtg tctttataaa atcggaacac tgatgtcaaa tattataaaa agacatactg
     4021 atcttgcggc aagatacggc ggtgaggaat tcggaattgt tctcggaaat atcgatgaac
     4081 atcacacttt tgaaatggca gaaagagtcc gaaagggaat atttgaactg aatattctgc
     4141 attctgcatc aagctatttc aacagccgga atgaaccggt tgtgacagtc agcataggag
     4201 cagccgttgt tattcctaaa agaaactccg agcctgagga actcattctg cttgccgatc
     4261 aggcgcttta caaagcaaag gcggacggaa ggaacagaac agttctatat actccggaaa
     4321 attcctgata tttatcatcc ttgctgaaag atatgcatac agcatcagta ataatacagc
     4381 acaataaagg aaaactatat gcctgaaaga ccctacatct attatgattt cactctgagt
     4441 ctgtgccccg tctgcctgaa gaggattgat gcaaaaattg taatgcagga ttcaggcgta
     4501 tatatgatga aaacctgttc agaacacgga agcttcaaag tactgatcga agaagatgaa
     4561 gtatattaca gaaatatcag aaattatgca aagagaagtg aaatgcctct gcattttgga
     4621 accgaggtta ttcatcgatg cccaatggac tgcggtctct gctccgacca tgaacagcat
     4681 tcctgtctga ctgtcctgga aattacagac agatgcaatc tggagtgccc gacctgctat
     4741 tccgcatctt ctccccgggc cggaagacac aggactcttt ctgagataga atttatgctg
     4801 gacaggatag tttataatga aggaagacc
//
LOCUS       DAOQGJ010000054         4805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9285_length_4805_cov_4.053263, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000054 DAOQGJ010000000
VERSION     DAOQGJ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9285_length_4805_cov_4.053263"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="PK683_01460"
     CDS             complement(<1..1470)
                     /locus_tag="PK683_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07139.1"
                     /translation="MSEFPIRSIAVYKHGIGFFSRRAKFENEEIRLKFRKEIMDDILK
                     SFTVIEHGEGKVLGVDFDTSETKEDKLKNTSVILEEGASLLHLLQSLRGKKVKLHLQD
                     NRMLTGILMGSDETKDEENWGTTRISVIQEGTKKIGIVQIGEVTELEILDSSSSSDLE
                     YFLNVSGAQETHCNITVRLTKSAHDLEVSYIAPAPLWTVNYRFVASKENKSLLQGWSI
                     FHNSLEEDLNQVRLSFITGMPISFIYELYNSFTPGRPHVREEERNVPAPITFAPPPKR
                     AEKKMREAEMAGDYPQEEMDDESSDDFADLSLPTLNSSVEVNTESRERDEAFQYTVSN
                     PVSVERGKSAMVPILSGNPEFRKEYIYNENKYKTHPSAVIRMKNTTGLTLEKGPIMIL
                     DEEGYSGEGMLDFTPKDAEAMIAYAIDMSVKVTRKNVTERTETAMNLKDRYITKEIYY
                     TFKSEYTVENKDSKPKTVFIEYQRGASNLSLIEPNTPDET"
     gene            complement(1658..2368)
                     /locus_tag="PK683_01465"
     CDS             complement(1658..2368)
                     /locus_tag="PK683_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HNH07140.1"
                     /translation="MKNLYNNKEPMEKENSSIKAVITGATGMVGDGVLMECLNHPNVT
                     EVLVIGRKKCGRVHPKMKEIVHSDMFDISLIADQLSGYNACFFCLGTTSVGKTEEEYN
                     RITTILAVDFAKVLLQKNSAMTFCFVSGAGTDGTEKSKTMWARVKGKAENELMKLPFK
                     KVFCFRPGFMDPSEGQNNVHSGYKYLGWIYPAARKIFPKYFSTMKEVALAMIHSVLYG
                     YEKQVLEVSDMVELAKRG"
     gene            2696..3091
                     /locus_tag="PK683_01470"
     CDS             2696..3091
                     /locus_tag="PK683_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07141.1"
                     /translation="MSFSAIYNQKTDIIECKVSGEISLLVLKEVFTAHARITAETGCT
                     KILNDYRDASVKLSTLEIYELPQMIAKIAASFGTDARRIRRALIVSKESEDYHFYETV
                     SANNSQKEKLFLDPEEARHWLIELPSGRP"
     gene            complement(3097..3507)
                     /locus_tag="PK683_01475"
     CDS             complement(3097..3507)
                     /locus_tag="PK683_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07142.1"
                     /translation="MKIHSIPGKLDVSWLEDVKAILDKWYSYSVSLAEFREATLEKGL
                     VHSKQNNGRAYIVDSSEATGVFTREIQDFIGTDIFPAYAAGGIQYFITISSEKSTLTD
                     MNVKTYSKKVGPAGIILVEVNSLNEAVDFLKNNP"
     gene            complement(3819..>4805)
                     /locus_tag="PK683_01480"
     CDS             complement(3819..>4805)
                     /locus_tag="PK683_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07143.1"
                     /translation="GKYDGEGTYFYADGNVYSGEFSDGLKNGNGKMKYADGSVYEGQF
                     KKDMIHGKGTYIFYYGDKYTGQFENGNREGKGTYTFNDGSVYEGEFKEDAFCGQGIYK
                     YSDGSIYTGEMKDSRKNGKGIYIYEDGGKYVGEFSNDEFNGNGTLTVGSDEYIGEFKE
                     DVFEGQGTLTYGNGDRYIGEFRKGEKEGIGTYLFSSGNKYVGEFRDSAMNGTGTFTYY
                     DGSVYTGEMKNNMCEGKGTLKYASGDTYTGDFKKDKRDGYGTYLKINGDKYIGEFQDD
                     LRNGKGTYTLADGDSYTGEFKDNLMHGQGVFTYSDGRKISGEFRNNEPVSSP"
BASE COUNT         1280 a         1093 c          857 g         1575 t
ORIGIN      
        1 agtttcatca ggggtattcg gctcaatcag actcaaatta ctggcacctc tctgatattc
       61 gataaagaca gtcttaggct tggaatcttt attctccaca gtatattccg atttgaaagt
      121 ataatatatt tcttttgtga tatatctgtc cttcaaattc attgctgttt ctgttctttc
      181 tgtcacattt tttcttgtga cttttacaga catatctatt gcgtaggcaa tcatcgcctc
      241 agcatccttc ggagtaaaat ccagcatccc ttctccggaa taaccttctt catccaaaat
      301 catgatagga cctttttcca gagtcagccc agtagtattc ttcattcgga tgacagcaga
      361 cggatgggtt ttatatttat tttcattata aatatactct tttctaaatt cagggtttcc
      421 agagaggatt ggaaccattg ccgattttcc tcgttctact gaaactggat tggaaacagt
      481 gtattgaaaa gcctcatctc tttctctcga ctctgtgttc acttccacag atgaattcag
      541 cgtaggaaga ctcagatcgg caaaatcatc cgatgattca tcatccattt cttcttgagg
      601 ataatcaccg gccatttcag cttctcgcat ctttttctct gcccttttcg gagggggtgc
      661 aaatgtgatc ggtgcgggaa cgtttctttc ctcttcccgt acatggggtc ttcctggggt
      721 aaaggaattg tagagttcat agatgaaact gatcggcatc ccggtaatga aagaaagacg
      781 cacctgattc agatcttctt ccagtgaatt gtgaaaaatt gaccatccct gcaaaagaga
      841 tttattttcc tttgaagcta caaacctgta attgacagtc caaagcgggg caggtgcaat
      901 ataggatact tccagatcat gtgccgactt agtcagacgt actgtgatat tgcagtgtgt
      961 ttcctgtgca ccggatacgt tcaaaaagta ttccagatcc gatgaagagg atgagtccag
     1021 aatttccaac tctgtgactt caccgatctg cacaatgcca atttttttag ttccttcctg
     1081 aatgactgag attctggtgg taccccagtt ctcttcatcc tttgtttcat cagaacccat
     1141 cagaatgcct gtcagcatcc tgttatcctg aagatgaagt tttacttttt tgcctcgcag
     1201 actctgcaga agatgaagaa gacttgcacc ttcttccaga atcactgatg tattcttgag
     1261 cttatcttcc ttagtttcag acgtatcaaa atcgacaccc agaacttttc cttctccgtg
     1321 ttcaatcacg gtgaaacttt tcaggatgtc atccataatt tctttcctga atttcagccg
     1381 gatttcttca ttttcaaact tggctctgcg ggaaaaaaaa ccgatcccgt gtttatagac
     1441 ggcaatggaa cgaattggaa attccgacat agtcattaat ccttaatata tattttgtta
     1501 ctgccagcat agaaaaagcc ggtgtcacat ttgtatttac cgctttttgc ggtcagcagt
     1561 ccaggttctg cagactcact gttttggcct caaagatcaa aatgagtacc tgtaggatta
     1621 gaaagaatct gttttttctg tcaagtaaat ttgcagatta acctcttttt gccagctcca
     1681 ccatatcact tacttccaga acctgctttt catacccgta caaaacagaa tgaatcatag
     1741 caagagcgac ttctttcatt gtggagaaat attttggaaa aatttttctt gctgcaggat
     1801 atatccatcc aagatatttg tagccgctgt gaacattatt ctgaccttct gaaggatcca
     1861 taaaaccggg tctgaagcag aatacttttt tgaacgggag tttcatcagc tcgttttctg
     1921 cctttccttt caccctggcc cacattgttt tgcttttctc agtaccgtct gtcccggctc
     1981 ctgaaacgaa acagaatgtc attgcactgt tcttttgaag gaggactttt gcaaaatcaa
     2041 cagccagtat agtggtaatt ctgttatact cctcttcagt ctttcctaca gatgtagtcc
     2101 ccaggcagaa gaagcaggca ttatatcctg aaagctgatc ggcaatcagg ctgatgtcaa
     2161 acatatccga atgaacaatt tccttcatct taggatgaac gcggccgcat tttttccttc
     2221 cgatgaccaa tacctccgtt acattcggat gattcagaca ttccatcagc actccgtcac
     2281 cgaccattcc agttgcacct gtaatgaccg cctttattga actgttttct ttttccatag
     2341 gttccttgtt attatacaga ttcttcattg agcgaaattt tctgaaccgc ttctgataag
     2401 taattcacag tatgcagaca gggtatccgg tcaagaaact ttcattccgt tcagtatgag
     2461 aagattctgt cgctttccgg cttaggtgag tctgttaaaa cctgaactca ggctgccgga
     2521 agagaataga tttcctgcag ggatatcaat taaagtgaac agatgttcgg ctgagtctga
     2581 atggataaat cttcatctaa tcaggagacg gcctgaacac cgaatatttt atattcagtt
     2641 atattgttgc cgtttttact gatcactgta tcactgactt aaaacggagg agattttgtc
     2701 tttcagcgca atatataatc aaaaaacaga tattattgaa tgtaaggtaa gcggcgaaat
     2761 ttctctcctc gtcttaaagg aagtattcac tgctcacgct cggatcactg ccgaaaccgg
     2821 atgtaccaaa atactgaatg attaccgtga tgcatcagtg aaactcagca ctttagaaat
     2881 ttacgaactt cctcagatga ttgcaaaaat cgctgcatcc tttggaacgg atgcaaggcg
     2941 gattcgaaga gcactgattg tctcaaaaga gtctgaagac tatcattttt atgaaacggt
     3001 ttcagccaac aattcacaga aagaaaaact tttcctggat ccggaggaag ccagacactg
     3061 gctcatcgaa cttccatccg gaagacccta agactgtcag gggttgttct tcaggaaatc
     3121 aactgcttcg ttcagactgt ttacttcaac cagaataatt cctgcaggtc caactttttt
     3181 ggagtaggtt ttgacattca tatccgtgag agtgcttttc tctgagctga tggtgataaa
     3241 atactgaatt ccgcccgccg cataggcagg gaatatatcg gttccgataa agtcctgaat
     3301 ttctcttgtg aaaactccag ttgcttcact tgagtcaact atataggccc ttccattgtt
     3361 ctgcttggaa tgaacaagac ctttctcaag tgtcgcttca cgaaactctg caagtgagac
     3421 actgtaactg taccatttgt ccagaattgc ctttacgtct tccagccagc ttacatcgag
     3481 ttttccgggt atagaatgaa ttttcataag tatactctac attaaacatt tttttctttc
     3541 ctttttcaag tcagaaaaga tctgaatcaa ttctaaggat tgttatcttt ctgtcaaaga
     3601 aaaatgggct tttttactga gttagttttt caaatagaga cagatgtaat tgtacattcg
     3661 caaactgaaa tgggtacgtc tgcatataat atgtcatttc aaatatggcc tctttttctc
     3721 ttttacagac gtgcattcag ttttgtgctg cgaaagaaca aaaaagaaat aaatgtatgc
     3781 agtattcagt aatttgaaaa aaatataact gaaatttatc agggagagct taccggttca
     3841 ttgtttctga attcaccgga aatttttctt ccgtcagaat atgtgaacac accctgaccg
     3901 tgcataagat tgtccttgaa ctctcctgtg taactgtccc cgtcagcgag ggtataggtc
     3961 ccctttccgt tcctgagatc atcctggaat tcgccaatgt atttatctcc gtttattttc
     4021 agataggtac catacccgtc tcttttatct tttttgaaat cgcctgtata cgtgtccccg
     4081 ctcgcatatt tcaaagttcc ttttccttca cacatattat ttttcatctc tcctgtatat
     4141 acagatccgt cataatatgt aaatgttcct gtaccgttca tcgcgctgtc tctgaattcg
     4201 ccgacatatt tattcccgct ggaaaataga taagtgccta taccttcctt ctcacctttc
     4261 ctgaattcac cgatgtaacg gtcgccgttc ccataggtaa gagttccctg tccttcaaaa
     4321 acatcttctt tgaattcacc gatatactcg tctgatccta cagtcagagt tccgtttccg
     4381 ttgaattcat cattactgaa ctcaccgaca tattttcctc catcctcata gatataaatt
     4441 cctttaccgt ttttacggct gtccttcatt tccccggtgt atatgcttcc gtcgctgtat
     4501 ttatagattc cctgaccgca gaaagcatct tctttaaatt ctccttcata aacactgcca
     4561 tcattaaagg tataagtgcc tttaccttct cgatttccgt tttcgaactg acctgtatat
     4621 ttatcaccgt aataaaaaat ataagtcccc tttccgtgaa tcatatcttt tttaaattgc
     4681 ccctcatata cacttccatc tgcatacttc atcttcccgt tcccattttt taatccgtca
     4741 gagaattcac cggaatatac gtttccatcc gcatagaaat atgttccctc cccgtcatac
     4801 ttccc
//
LOCUS       DAOQGJ010000055         4756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9435_length_4756_cov_4.099553, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000055 DAOQGJ010000000
VERSION     DAOQGJ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9435_length_4756_cov_4.099553"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3106
                     /locus_tag="PK683_01485"
     CDS             <1..3106
                     /locus_tag="PK683_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH07144.1"
                     /translation="LESEHSPGKYISGEPGKLGFSDYSHNHISYSRKKEDRMITLRSE
                     IAYSESSYLVIPPTEYPLNMYVNGHSVFLRGNFQETYSSRIHFSSAVSVPKEFLNRIG
                     KNEIAFELYPREGETLPFAKVFISDRKDAYNYVFWRNILGPHLLQAISICSLVFFFYF
                     MTLYIYNPERMHFLYFGLLCFTFIFSYSNNYLSSDYTDTLILEKTVRTAFPLSNAFGL
                     AFLLEYTNVTRWNKLFVLIAMAFYGAASLVPLFAENLEILRGISSFFIIPVSAAGNIS
                     GIIICIIYYIVQRNSISLSFLLMTVFGLCITGHDIYYFAGAGIKPYVMLVPIYTFFYI
                     LFIFFLLAKEQSEIQKGSVLNEKKLTALNENLEKIVEARTEEIQKQITSLNREIEIRI
                     KVEQLLKKLTAAVEQSPVSIVITDRNGVTEYVNPTFSSITGYSPEEVIGQNQNILKGE
                     TSSEVYQDLWKTVMSGKIWKGELINRKKNGDLYYEETVISPITNFNNELINFIAVKRD
                     VTEDKTIQSQLNILATAIEKSPIVIVITDIHGVIEYVNPVFSTVSGYSYSESVGKSTR
                     MLKGETPSYIIKELWNTILSGNVWKGEFINKRKNGDLYYEEATIAPIMDSSGKIFNFI
                     ALKSDISKRKYTEQKLMETEKKFEQLITAVPISLMFVKNNGEVEFHNDRFSELFGYTL
                     QDIPTVNDWWRLAFPQTEYRNRAMFIWNQAVEDALGGSRYIEPLIFHISCRSEEIKTV
                     EISGLLTEKGYLAAFTDITERMQNLEKLEKQHKDLKAAQTQLVHSEKMAALGTMVAGV
                     AHEINNPVNYVYLSSEALQRDLGTFKKEIQEMTDSEDEETASYFNDYFAKFHKSIDNI
                     LEGSGRIKTIVQDLRIFSRLNEAEKKSIFLQEAVESSLRIVKTQYKEEFEFIKNFRSE
                     RIVECYPAQLNQVFLNILINGCQAVHKRINDTEDKSAGIIRMDLYDIYKETVVSFSDN
                     GIGMNEEEKKRVFEPFYTTKPVGHGTGLGMSISYSIIEKHNGYIKVQSEKGRGTVIEI
                     HLPS"
     gene            complement(3101..3775)
                     /locus_tag="PK683_01490"
     CDS             complement(3101..3775)
                     /locus_tag="PK683_01490"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNH07145.1"
                     /translation="MKIIATVSEQDILSLDRYDTSCVDVLEIRLDLCSEKFLSENFSS
                     SLSDWKGALLFTYRNPEDSSIKDQHIQRKDFIEKFLEPFNSENNFLDIDMKRDHGMFE
                     KYEISNYKIIYSVHNFDGIISYTEMNIFSEPKRKTNPDSIFKFAAFPKSAAESVQFLS
                     AVKAFSVKAAVIGIAMGAEGLYSRIFGDRFGSSMTYCCLDEPKAPGQIKAADLKRIRI
                     LSELDL"
     gene            complement(3778..4353)
                     /locus_tag="PK683_01495"
     CDS             complement(3778..4353)
                     /locus_tag="PK683_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07146.1"
                     /translation="MIFFVFMNRKTSFFRKFHIQYIILFTILCFTALCTNPIEKKKKQ
                     LENCKITLDSVKIAKYRMLLIPPVPKIYFKAKVNVENANPETVTLQKFDFKVMTNRKS
                     QNNSTIAEVQSTEEHELQAGEIKALELNLVTMLEETADEEIVGLVIKIFKSLIMNEEL
                     EFLLKGNVHFDTAVGKLSIPVEQVFKTKARL"
     gene            complement(4385..>4756)
                     /locus_tag="PK683_01500"
     CDS             complement(4385..>4756)
                     /locus_tag="PK683_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNH07147.1"
                     /translation="ENFVQKEGISTGVLAPYSSQVYLLREKFKLLTDKYPLLSVNTID
                     SFQGSEKEAILISMTRSNEKGNTGFLSEYKRLNVAMTRARKKLIIVGDSITLTADPVL
                     KKIIQLAEKKGHIRSCYEFME"
BASE COUNT         1431 a          885 c          976 g         1464 t
ORIGIN      
        1 cctggaatcg gaacattctc ccgggaaata catttcaggc gaacctggaa aacttggctt
       61 ttcagactat agccataatc atatcagcta ttcacggaaa aaggaagaca gaatgattac
      121 tctccgttcc gaaattgcct attccgaaag ctcctatctt gtgattccgc cgacagaata
      181 tcctctgaat atgtatgtaa acggtcattc tgtgtttttg agagggaatt ttcaggagac
      241 ttattccagc agaattcatt tcagttcagc agtatcagtt cccaaagagt ttctgaacag
      301 aataggaaaa aatgaaatcg catttgagct ttatcccagg gaaggagaaa ctctgccgtt
      361 tgcaaaggtt tttatttcag acagaaagga tgcatacaat tatgttttct ggaggaatat
      421 tctcggtcct catctgctgc aggcaatcag catctgcagt ctggtctttt ttttctattt
      481 tatgacattg tatatttata atccggaaag gatgcatttt ctatattttg gacttctctg
      541 ctttacattt attttttcct attccaacaa ctatctttct tctgattata cggacactct
      601 gattcttgaa aagacagttc ggacggcatt tccattgagc aatgctttcg gtcttgcctt
      661 tcttctcgaa tacacaaatg tcaccagatg gaataaactc tttgttctga ttgcaatggc
      721 attttacggg gcagcatctc ttgttccctt gtttgcagaa aacctggaaa tactgcgggg
      781 aatcagcagt ttcttcatta ttcctgtcag tgcagccgga aatatatcag gaataattat
      841 atgtataatt tattatattg ttcaaagaaa ttcaatttct ctttcttttc tgcttatgac
      901 agttttcggt ctctgcatta ctggtcatga tatctattat tttgcgggtg caggcataaa
      961 accttacgta atgcttgttc caatatatac tttcttctac attcttttta ttttctttct
     1021 tctggccaag gaacagtcag agattcagaa aggatcagtg cttaacgaaa aaaaactcac
     1081 tgcactgaat gaaaatctgg aaaaaatagt ggaagccaga actgaggaaa ttcagaagca
     1141 gatcacttcc ctgaacaggg agatcgaaat cagaattaaa gtggagcagc tcctgaaaaa
     1201 gctgactgct gcagtagagc agagtcctgt gtccattgta attactgaca gaaacggtgt
     1261 tacggaatat gtcaatccta ctttttcctc aatcaccggt tacagtcccg aagaggtcat
     1321 aggtcagaat cagaatattc tgaaagggga gacatccagt gaagtctatc aggatttatg
     1381 gaagactgtc atgtcaggaa aaatctggaa aggagagctc ataaacagaa aaaagaacgg
     1441 agatctttat tacgaagaga cagtcatttc tccgatcaca aattttaaca atgagctgat
     1501 caactttatt gcagtgaaaa gagatgtaac agaagacaaa acgatacaga gtcagctgaa
     1561 tattcttgct actgccattg agaaaagtcc tattgtaatc gttattactg atattcacgg
     1621 tgtgatagaa tatgtaaatc cggtattcag cactgtttcc ggttattcgt attcagaatc
     1681 tgtaggaaaa agcaccagga tgctgaaggg agagactcct tcatatatca ttaaggagct
     1741 ttggaatact attctttcag gaaatgtatg gaaaggagaa ttcattaata agaggaaaaa
     1801 cggggacctt tattatgaag aagcaaccat tgctccgatt atggattcat ccggaaagat
     1861 atttaatttt attgcattaa aaagtgatat atcaaagaga aagtatactg agcagaagct
     1921 tatggagacg gaaaagaaat tcgaacagct gataactgct gttccaattt cactcatgtt
     1981 cgtaaagaat aacggtgagg tggaatttca taacgaccga ttttcagagc ttttcggata
     2041 cactctgcag gatatcccga cagtcaatga ctggtggaga cttgcttttc ctcagactga
     2101 gtacaggaac agggcaatgt ttatctggaa tcaggctgtg gaggacgcgc tgggaggaag
     2161 cagatacatt gaacctctta tctttcatat atcatgcaga tcagaagaaa tcaaaactgt
     2221 ggaaatatcc ggtcttctta cggaaaaggg ataccttgca gcatttacag acattacaga
     2281 aagaatgcag aatcttgaaa aactggagaa gcagcacaag gatttgaagg ctgctcagac
     2341 tcagcttgtt cattcagaaa agatggcggc cctcgggaca atggttgcag gagttgctca
     2401 tgaaatcaat aatcctgtaa attacgttta tctgagttca gaggctctgc agagagatct
     2461 gggaaccttc aagaaagaaa ttcaggaaat gacagattca gaagatgaag aaaccgcttc
     2521 ctattttaat gactattttg caaaatttca taaatccatt gataatatac tggaagggag
     2581 cggaaggata aaaacaatcg ttcaggatct taggattttt tccaggctga atgaggcaga
     2641 gaaaaaaagc atctttcttc aggaagcggt agaatcttct ctcaggattg taaagactca
     2701 gtacaaggaa gaatttgaat ttattaaaaa cttccgttca gagcggattg tggaatgtta
     2761 tcctgctcag ctgaatcagg ttttcctgaa tattctcatc aacggatgcc aggccgttca
     2821 caaaagaata aatgacacgg aagacaagtc tgcgggaatt atcagaatgg acctctatga
     2881 tatttataag gaaacagtag tgtctttttc tgataacgga attggaatga acgaggaaga
     2941 aaagaaaaga gtctttgagc ctttttatac caccaagccg gtaggtcatg gtacaggtct
     3001 gggaatgtct atttcctaca gcatcattga aaagcacaat ggatacatta aggttcagtc
     3061 agaaaaaggc agaggcactg tcatagagat tcatctccct tcataagtca agttcagaaa
     3121 gaattcttat ccttttcaga tccgccgctt tgatctgacc tggagccttc ggttcatcca
     3181 gacagcagta tgtcatactt gagccgaatc ggtctccaaa aatcctggaa tataatcctt
     3241 ctgcacccat tgcgattccg atgacagccg ccttgacaga aaatgctttt actgcgctga
     3301 gaaactggac tgactcagct gccgacttcg gaaatgcagc aaacttgaaa atggaatctg
     3361 gatttgtttt tctctttggt tccgagaaga tgttcatttc agtataagat atgattccgt
     3421 caaaattatg aacagaataa atgattttat aattagaaat ttcatatttt tcaaacatcc
     3481 catgatctct cttcatgtca atgtccagaa agttattttc tgagttgaac ggttccagga
     3541 atttttctat aaagtccttt ctctggatat gctgatcctt tatgctggaa tcttccgggt
     3601 ttctgtaggt aaacagcagt gcgcctttcc agtctgaaag cgaactggaa aaattttcgg
     3661 agagaaattt ttcggaacat aggtccagac ggatttccag cacgtcaacg caggaagtgt
     3721 cgtatctgtc cagactgagg atatcctgtt ctgaaactgt tgctatgatt ttcataatca
     3781 gagtcttgcc tttgtcttga atacctgctc gaccgggatg ctgagtttcc ctactgcagt
     3841 gtcaaaatga acattccctt tcaacagaaa ttccagctct tcattcataa ttaaagattt
     3901 aaatatttta atgacaagtc ctacaatctc ctcgtcggca gtctcttcaa gcattgtcac
     3961 caggttcagt tccagtgcct tgatttcacc cgcctgaagt tcgtgttctt cagtgctctg
     4021 cacttctgca atggtggaat tgttctgtga ttttctgttt gtcatcacct tgaaatcgaa
     4081 tttctgaagc gtgactgttt caggatttgc attttccaca ttgacttttg ccttgaagta
     4141 aatcttgggt acagggggga taagaagcat tctgtatttt gctattttta ccgagtcaag
     4201 agtgattttg cagttttcaa gctgtttctt ttttttttct atggggtttg tacataaggc
     4261 cgtgaaacat aatatagtga ataatattat atattggata tggaatttcc ggaagaatga
     4321 ggttttcctg ttcataaaga caaaaaatat cattctgaat ctgaagtcca gtattaaact
     4381 aagcctattc catgaattca taacaggagc ggatgtgtcc ttttttttct gcaagctgaa
     4441 tgattttttt cagtacagga tctgcagtga gagtgatgct gtcaccgaca ataatcagtt
     4501 ttttcctggc tcttgtcatt gccacgttca gtcttttgta ttcggacaga aatcctgtat
     4561 ttcctttctc atttgatctg gtcatactga taaggattgc ttctttctca gaaccctgaa
     4621 atgaatctat agtattcact gataataaag gatatttatc agtaagaagc ttgaactttt
     4681 cccggagaag gtagacctga gaagagtagg gtgcgagaac ccctgttgat attccttctt
     4741 tctgcacaaa gttttc
//
LOCUS       DAOQGJ010000056         4750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9454_length_4750_cov_2.932481, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000056 DAOQGJ010000000
VERSION     DAOQGJ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9454_length_4750_cov_2.932481"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PK683_01505"
     CDS             complement(<1..296)
                     /locus_tag="PK683_01505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH07148.1"
                     /translation="MANAVIKTSMGEFSVYLDEERAPVTAGNFIKLAKAGFYDGLIFH
                     RVIKNFMIQGGCPKGNGTGGPGYKIQDEFHPELRNKAYTLSMANAGPNTGGFQF"
     gene            complement(296..1117)
                     /locus_tag="PK683_01510"
     CDS             complement(296..1117)
                     /locus_tag="PK683_01510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNH07149.1"
                     /translation="MKKEILRRKEIILRALPDIGKYLKFMQSKNDLEISRKKNLVDLV
                     TEADKGSEEMIVKAVLNEFPDDHILGEEGGTRQGKSDFRWVIDPLDGTTNFTHKLPLY
                     AVSIGLEYIPERKICAGFVLLPALDELYHAVQDEGSYKNDKRIYVSSTEKMIEALFCT
                     GFPYRREDNTDRILEYLKNMLVLTRGIRRTGAAALDLCWVAEGRFDGFWEESLSPWDI
                     AAGGFIIQQAGGKVSTFDGNSFSLDMPNILATNAKIYEKALLEFKENLSKRMEHY"
     gene            complement(1114..1875)
                     /locus_tag="PK683_01515"
     CDS             complement(1114..1875)
                     /locus_tag="PK683_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH07150.1"
                     /translation="MIHGKKLVIVLPAYNAEKTLEKTYKEIPHEIVDEVILVDDRSKD
                     GTVDEAKRIGIRYVIVHEQNKGYGGNQKTCYNKAKELGADIVIMLHPDYQYTPKLITA
                     MASIIALDLYPVVFGSRILGKGALNGGMPAYKYIANRLLTFSQNLLLNQKLSEYHTGY
                     RAFSKAVIDTVDYEKNSDDFVFDNQMIAQIFAAGFEIAEVTCPTKYFAEASSINFKRS
                     VKYGFGVLSVSFAYFFYRIGLFKKDWLKPKGTSVK"
     gene            complement(1875..2810)
                     /locus_tag="PK683_01520"
     CDS             complement(1875..2810)
                     /locus_tag="PK683_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNH07151.1"
                     /translation="MEKNAKIYVAGLYGMVGSAIKRVLQEKGYTNIIGHSSEELNLIR
                     QSDVEKFFKTERPDYVFISAAKVGGIYANNTYPADFIYNNLQIQNNLIHSSYVYRVKK
                     LLFLGSSCIYPKFAEQPIKEDYLLSGKLEPTNEAYAIAKIAGVKMCEFYNSQYHTNFI
                     SAMPTNLYGIGDNYNAMNAHVIPALIRRIHEAKKSNAKEVVIWGTGKPLREFLFVDDL
                     ADACVFLMHEYLSNQTINVGSGNEVSILELANLIKKAVGYSGELVFDSSKPDGTPRKL
                     LDTSRIHGLGWKPKTDLEAGLKVAYEDFLKGNVRL"
     gene            complement(3137..3313)
                     /locus_tag="PK683_01525"
     CDS             complement(3137..3313)
                     /locus_tag="PK683_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07152.1"
                     /translation="MSCSLIKNKSDYKDLGMEYFEQKYRYRNTASLQRRAALGLSVLE
                     NPSEFPKVCFLGEI"
     gene            complement(3652..4509)
                     /locus_tag="PK683_01530"
     CDS             complement(3652..4509)
                     /locus_tag="PK683_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH07153.1"
                     /translation="MKRILILGLLILGISVQGSPEEELFNSVTKGNIPAVKKALEQNP
                     QLGRKYNFGKTVLFFAVEGGKEEIVQMILDYSAVSGSNSLTADLDDNQQSAGHYAAAR
                     GHLPILKLLISRGLDMLQKNRQGQNILDIAQSYGHSEVVSFLEVEKNIRPNRKVEKDG
                     LALGLYTIYLLISIALTVWVAKTLSVNGRIFLIDSLSNEKLADSVNHLLVVGFYLLNI
                     GYITLALKIEHKPDSFVQSLELLSTKIGFVLVILGIIHFFNIFMFGRLRKKTSEKRAE
                     RERSAVSSI"
BASE COUNT         1309 a         1083 c          883 g         1475 t
ORIGIN      
        1 aaaaactgga aaccgccggt attgggtcct gcatttgcca ttgaaagagt atatgccttg
       61 tttctcagct caggatgaaa ttcgtcctgt atcttgtatc ccggaccgcc tgttccgttt
      121 cctttcggac agccgccctg aatcataaaa ttctttatta ctctgtgaaa tatgagtccg
      181 tcataaaacc cggcctttgc cagttttata aaatttcctg ccgttacagg tgctctttcc
      241 tcatccagat aaacgctgaa ctctcccata gaagttttta tcactgcatt agccatcagt
      301 aatgttccat ccttttgctt aaattttcct taaattcgag taatgctttt tcgtatattt
      361 ttgcattggt tgccagaata ttcggcatat ccaaggaaaa ggaatttccg tcaaaggtgc
      421 tgacctttcc ccctgcctgc tgaataatga accctcctgc tgcaatatcc catggagaaa
      481 gactttcttc ccagaagccg tcaaaacgtc cttctgcaac ccagcaaaga tcgagagctg
      541 ctgcaccggt tcttctgatt cccctggtaa gaaccagcat attcttcaaa tattcaagta
      601 tcctgtcagt attgtcttcc cttctgtaag gaaatcccgt gcagaagagg gcttctatca
      661 ttttttcagt ggaagaaaca tatattcttt tatcattttt atatgagcct tcatcctgaa
      721 cagcatggta cagttcatcc agggctggaa gcagtacaaa cccggcacag atttttcttt
      781 cagggatata ttccagtcca atggaaacag cgtaaagggg aagtttatga gtaaaattcg
      841 tagtcccgtc cagaggatca ataacccatc tgaaatcaga cttgccctgc ctagtaccgc
      901 cttcctcgcc caggatatga tcatcaggaa attcgttcag cactgccttg acaatcattt
      961 cctcggaacc tttgtccgct tcagtgacca gatctaccag attttttttc ctgctgattt
     1021 caaggtcatt cttcgattgc ataaatttta gatattttcc aatatccggt aatgctctta
     1081 gaatgatctc ttttcttctc agtatttctt ttttcatttt actgaggttc ccttcggttt
     1141 gagccagtcc tttttaaaaa gccctattct gtaaaagaaa taggcaaaac tgacactcag
     1201 cactccaaat ccgtatttta cgcttctttt aaaattaatt gacgatgcct cagcaaaata
     1261 tttcgtagga catgtgactt ctgcaatttc gaaaccggca gcaaatatct gtgcaatcat
     1321 ctgattgtcg aatacgaagt catcggaatt tttttcgtaa tcaaccgtat caataacagc
     1381 tttggagaac gcacggtaac cggtatggta ttcagaaagc ttctgattca aaagcaggtt
     1441 ttgagaaaaa gtcagcagtc tgtttgcgat atacttgtat gcaggcattc cgccgttcag
     1501 tgctcctttt cccagtatcc ttgacccaaa caccacagga tacagatcga gtgcaatgat
     1561 gcttgccatt gctgtgatga gtttgggagt atactgataa tcaggatgga gcatgattac
     1621 aatgtcagct ccgagctcct tggctttatt atagcaggtc ttctgatttc cgccataacc
     1681 tttgttctgc tcatgtacta taacatatcg tatgccgatt cgctttgcct catctactgt
     1741 gccatccttg cttctgtcat ccaccagaat aacttcatcc acaatttcgt gaggaatttc
     1801 tttataggtt ttttccaaag tcttttctgc attgtatgca ggaagaacga taacaagttt
     1861 ttttccgtga atcatcagag tctcacattg cctttcagaa agtcctcgta ggccactttg
     1921 agtcctgctt ccagatctgt ctttggtttc catcccagcc catgaattct ggaagtatcc
     1981 agaagctttc ttggagtacc gtcaggtttg gacgaatcaa acacaagttc tcctgaataa
     2041 cccacagcct ttttgataag atttgccagt tcaagaatgc ttacttcatt cccgctcccg
     2101 acattgatcg tctgattgga aagatattca tgcataagaa aaacacaggc atccgccaga
     2161 tcatcgacaa agagaaattc gcggagaggt ttgcctgtcc cccaaataac gacttccttt
     2221 gcatttgact tttttgcttc atgaattctt cggataaggg ctgggatcac atgagcattc
     2281 attgcattat aattatcacc tatcccataa agattagtag gcattgctga aataaaattt
     2341 gtatggtact ggctgttgta gaattcgcac attttgacgc ctgcaatttt tgcaattgca
     2401 taggcctcat ttgtaggttc aagttttcct gaaagaaggt agtcctcctt gatcggctgt
     2461 tctgcaaatt ttggataaat gcatgatgag cccagaaaaa gcagtttttt cactctgtaa
     2521 acatacgaag aatggatcag gttgttctgg atctgaagat tattgtagat aaaatctgcc
     2581 ggataagtat tgtttgcata aattccgcct acctttgcgg cagaaataaa gacataatcc
     2641 ggtctctctg tcttgaagaa tttttctaca tctgactgtc ggattaaatt gagttcctcc
     2701 gaactgtgac caataatatt tgtatatcct ttttcctgga gcactctttt aatagcagac
     2761 cctaccattc cataaagtcc tgccacataa atcttagcgt ttttttccat aggttccttt
     2821 ttttgcgggg attcctttcc cgtaagcaga aatttcctct gtttttagtc tttttgccgt
     2881 gcgctaaaaa taaaactatt tttttagttg aaacagaaag gaaactgagc aaaagcagat
     2941 atttcagtcc gtatttatga actgcagttc aaacttttat tccgtgaaaa atacagagaa
     3001 aggagaaaat caatcttttt ccgctctaca gcaaaattct ccctttaaac cgatttatcg
     3061 gtacgaagag gtattaatgg aggcagagtt tcacggcaaa ctgtagagta attgtgatga
     3121 ttatgcccag tctttctcag atttccccta agaaacagac cttcggaaat tcagaaggat
     3181 tctcaagaac cgagagacca agagcagctc tcctttgtaa tgatgctgta tttctgtatc
     3241 tgtatttctg ttcaaagtat tccattccca gatccttgta atccgattta ttctttatca
     3301 gactgcaaga cataagtacc agttttcctg tacaggcaga atttgccttc cggcacctcc
     3361 acttttctgt aataagcgcc gaaatagctc cggctctgct gcgtgtccag aacgtcttac
     3421 tgagtgcatc agcatctcag cttccataat actgaatgtc gcactgcaga ctgcgagaga
     3481 ctagagaaaa gcgggaattc aagaggcttc ccaacttgca aaacgaacgc aggcaggaga
     3541 ttttccctaa tcagcctgcc cgaaattttc atgaatttgt cattataaaa attgcagaac
     3601 cggagaactt cagtcagaaa ttctgaggct ctgctgctga tcactgaaaa atcatatgct
     3661 gctcaccgca gacctctctc tttcagctct tttctccgat gttttttttc tgagtcttcc
     3721 gaacatgaat atattgaaaa aatgaatgat gcccagtatc acaagaacaa atccaatctt
     3781 tgtgcttaaa agctcaagag actgaacgaa cgaatccggc ttatgttcta ttttcagcgc
     3841 gagtgtaata tatccgatat tcagaagata aaaaccgacc accagaagat gatttacaga
     3901 atctgccagt ttttcattcg acagactgtc gatcaggaaa atccttccgt ttacactgag
     3961 ggtctttgcc acccagacag tcagtgcaat gctgataaga agatagattg tataaagtcc
     4021 gagtgccagt ccgtcttttt ccactttcct gttcggccgg atattttttt caacttccag
     4081 gaaactcaca acttccgaat gaccgtaact ctgcgcaata tccagtatgt tctgcccctg
     4141 tctgtttttc tgaagcatat ccagaccgcg tgaaataagc agtttcaata tggggagatg
     4201 acctctggct gctgcataat gcccggcaga ctgctgattg tcatccagat cggcagtcag
     4261 agaattgctt cccgacactg cggaatagtc cagaatcatc tgaacaatct cttcttttcc
     4321 gccctcgact gcaaaaaaca gcactgtctt tccaaaatta tattttctgc caagctgggg
     4381 attctgttcc aaagcctttt tcactgcagg aatatttccc tttgtcacag aattgaagag
     4441 ctcttcttca ggcgaacctt gaacagatat gcctaaaatt aaaagaccca gaatcagaat
     4501 tcgtttcata aaatcctcca tttgaacatg ttcaaattta tatcataaaa caaaatctgc
     4561 aatcactttt ttgaacgcgt tcagtttttt ttcagttttt cagatgaact ctggaaaccc
     4621 ggatgaaagt tccgccagta actgaatact caagttccgc ctgacccaga ctgagtgcag
     4681 gaagaacgag actgcagtac ggcatagaga gacctgtatt gatctggatt tcctggaaga
     4741 caaaaaccct
//
LOCUS       DAOQGJ010000057         4745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9466_length_4745_cov_2.965672, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000057 DAOQGJ010000000
VERSION     DAOQGJ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9466_length_4745_cov_2.965672"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2095)
                     /locus_tag="PK683_01535"
     CDS             complement(<1..2095)
                     /locus_tag="PK683_01535"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004778022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07154.1"
                     /translation="MLSAYPSYKCISKMVTNSQAAGSLWIALVSSLIFFSAMNVLTLF
                     NTAEEASVSWRFRLTKGKKGKGSSSLDGKGFVEKFDPPENARKDIRIIGIKTETVNKL
                     QWPIKWQEYAKIIKAFKKTSNWLFFDIFFIDYKQNGQVEAMADALTGSKNVLLDYSVE
                     TDSSSKASTVNFEERLEILRKYRLKNVVDPQDKGITWLELAVPPIAPIASKVNGLGFA
                     NINKEEGISNRRMPLVAKLKGHGPNRETEFYPSIDLVTVCAYYGVDVVKDTEVVMGKY
                     IKIKNIPNKQIAGKDIMYAPNPEREIVIPIDYYGQMLINFVGALYSFQDEDLYDVAND
                     WTEETAAQFENTIFLIAMYYATGIGAAKDTHLSPHGEMSGIEHHAHTLNTILNQDFIK
                     ESSKFLNLFLILFFGLTVGVLQHRLHTAASFGLWFFMSVIYLVLAFTLFFKFNFIIPV
                     TTPLITMSFVFVGIIGYKILTEEENVKYIRNTFSKFVSKDVVDELLRDPDKIALGGSR
                     KEITVFFSDVRGFTTLSEALSPEDLVKLLNEYLSVMTNVIIDFKGTIDKYMGDAIMAF
                     WGAPINMEDHPYYACVSAIVQLNKLKELQADWKSRNLPSIDIGIGLNTGPAVVGNMGS
                     SHRMDYTCMGDTVNLGSRLEGSNKMYATRIIISEYTYERVKERVYARELDLVKVKGKN
                     QPVRIYELIGLVDDND"
     gene            complement(2238..3056)
                     /gene="trpA"
                     /locus_tag="PK683_01540"
     CDS             complement(2238..3056)
                     /gene="trpA"
                     /locus_tag="PK683_01540"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNH07155.1"
                     /translation="MKFQEMFAKKEVLFIPYFSLGDPSFHDCVHWADALIDGGADILE
                     LGIPFSDPIADGPVIQKSYKRALDHTPFQMDLVYKATEVIHKKHPNIPLMYLTYYNPI
                     QRVGVENFLNTVSELGVQGIVIPDLPFDTHDFHNVRQTADKVGISLIQLITLATHKER
                     IRQISENASGFIYFVTSYGVTGERRDFDPKLKEKITMVKKVSGIPVSAGFGISNAEQA
                     EFVSGFADGVIIGSAVQRIIELQGNSAEDCKQSLTAFAKEIKQACVRGKNVGKK"
     gene            complement(3053..4252)
                     /gene="trpB"
                     /locus_tag="PK683_01545"
     CDS             complement(3053..4252)
                     /gene="trpB"
                     /locus_tag="PK683_01545"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNH07156.1"
                     /translation="MNVSPFDKNQGYFGEFGGRYAPEILTEALFQLEDTYNKLKKDKD
                     FNKKLLYYQKNYIGRPSILTYAERLTKIWGGAEIFLKREDLNHTGAHKINNTVGQGLL
                     ALAMKKKRIIAETGAGQHGVATATVGALFGLEAVVYMGTEDLRRQELNAVRMKMLGAK
                     VIGVSAGTATLKDATSEAMRDWALNVGNTHYIVGSAIGPHPFPTIVRDFQSLIGKESK
                     AQFKAVRKKLPDAVVACVGGGSNAIGMFYGFLKDKSVELYGVEAGGRGTAPGEHSATI
                     TFGKPGFFHGTKTLLIQDEDGQVVPAHSVSAGLDYPGVGPEHAYLAETKRVKYVSVTD
                     EEALDAFLEVCKLEGIIPALESSHAFAYAKKIAKKLGKKKSMIICLSGRGDKDVAEVQ
                     RLRKDIL"
BASE COUNT         1295 a         1105 c          932 g         1413 t
ORIGIN      
        1 cgtcattgtc atctacaagt ccgatcagtt cataaattcg tacaggctga tttttccctt
       61 ttactttcac gagatcaagt tcccttgcat acactctttc ttttactctt tcataagtgt
      121 attcagaaat aatgatcctt gttgcataca ttttattgga gccttccagt ctcgaaccga
      181 gattcacagt atctcccata caggtatagt ccatcctgtg agaacttccc atatttccca
      241 caaccgccgg tcctgtattc agcccaatac ctatatcgat ggaaggaaga tttctcgatt
      301 tccagtctgc ctgcagttcc ttcagtttat tcagctgaac aattgcggac acacaggcat
      361 aatagggatg atcttccata ttgataggtg caccccagaa agccataatg gcatcaccca
      421 tatacttgtc aattgtgccc ttgaagtcaa taattacgtt tgtcattacg gaaaggtatt
      481 cattcagaag tttgacaaga tcttccggac tcagtgcttc cgagagagtc gtaaacccgc
      541 gcacgtcgga gaagaatacg gtaatttcct ttctggatcc tcccagggca atcttgtccg
      601 gatcacggag aagttcatcc accacatcct tggatacgaa tttggagaat gtattcctga
      661 tgtacttcac gttttcttct tcagtcagaa tcttatatcc gataattccc acaaacacga
      721 aagacattgt gatcagaggt gttgtgaccg gtataatgaa attaaactta aaaaataatg
      781 taaatgccag aacgagataa atgacactca taaaaaacca gagtccgaaa ctggctgcag
      841 tatgaagcct gtgctgaaga actcctacag tgagaccgaa gaaaaggatg aggaacagat
      901 tgagaaactt tgaactctct ttgatgaaat cctgattcag aatagtgttc agagtgtgag
      961 catggtgttc aatcccggac atttcaccgt gaggagaaag gtgtgtatcc ttggctgctc
     1021 cgatccctgt cgcgtagtac attgcaatca ggaaaatggt attttcaaac tgtgctgcgg
     1081 tttcctcagt ccagtcattt gccacatcat acaggtcttc atcctgaaag gaatacagtg
     1141 ctcctacaaa gttgatgagc atctgaccgt aataatctat aggaattaca atctctcttt
     1201 cagggttggg agcatacatt atatcctttc cggcaatctg cttattcggg atatttttta
     1261 ttttgatgta ttttcccata accacttctg tatccttgac tacatcaact ccgtagtagg
     1321 cacatacagt cacaaggtct atggaaggat aaaactcagt ttcacggttg ggtccgtgtc
     1381 ctttgagttt ggcgacaaga ggcatccttc tgttgctgat tccttcttcc ttgttgatat
     1441 tcgcaaatcc gagaccgttc accttggaag caattggggc aatcggagga acagccagct
     1501 ccagccaggt aattcccttg tcctgaggat caactacatt tttcagtctg tattttctga
     1561 gaatttccag tctttcctca aaattgacag tggaagcctt tgaagaagaa tctgtttcga
     1621 cggaatagtc aagaagcaca ttctttgaac ctgtcagtgc atcagccatt gcttccacct
     1681 gaccgttctg cttgtagtct ataaagaaaa tatcgaaaaa gagccagttg gaagttttct
     1741 tgaatgcctt gatgattttt gcatattcct gccatttgat cggccactga agtttattga
     1801 cagtctctgt cttgattcca atgatgcgaa tgtcctttct ggcattttca ggagggtcaa
     1861 atttttccac aaatcccttt ccgtccagtg aagaagaacc ctttcctttc tttcctttag
     1921 tcagtcggaa tctccaggaa acagaagcct cttccgctgt attgaacagt gtaagaacat
     1981 tcatcgcaga gaaaaatatc agagaggata caagggcaat ccataaactt cctgcagcct
     2041 gactgtttgt caccatcttc gaaatgcatt tgtatgaagg gtaggctgag agcagaagca
     2101 taaccaggct gatttcgaga atagtcagaa agtttacaga ctcttcataa atcggagaga
     2161 atataactcc aagcgatcca atgacagcga ttccaagtgc aagatacaga ttcagaccga
     2221 tatgaacgtg ctgcttctta tttttttccg acatttttcc ctctcacaca ggcttgttta
     2281 atttctttcg caaaagcagt caagctttgt ttgcagtctt ctgcagaatt tccctggagt
     2341 tctatgattc tctgtactgc tgagcctata atgactccat cagcaaaacc ggatacaaac
     2401 tctgcctgct ctgcgttgga aattccaaat cccgcagaaa ccggaatgcc ggacactttc
     2461 ttgaccatcg taatcttctc tttcagtttc ggatcaaagt ctctgcgttc tccggtcact
     2521 ccatatgagg tcacaaaata gatgaatccg gatgcatttt cagaaatctg tctgattctt
     2581 tctttatgtg ttgccagagt aatcagctga atcagagata ttcccacttt gtcggcagtc
     2641 tgtcttacgt tatgaaaatc atgagtatcg aaaggaagat cagggattac aatgccctga
     2701 acgcccagtt cagatacggt attgagaaaa ttttccactc cgactctctg aataggattg
     2761 taataagtga ggtacatcag cgggatattc ggatgttttt tatgaatgac ttcagtagct
     2821 ttgtacacaa ggtccatctg aaaaggagta tgatccagtg ctcttttata ggatttctga
     2881 atgacaggtc cgtctgcaat gggatctgaa aacggaattc ccagttccag aatatcggca
     2941 ccgccgtcaa tcagtgcatc cgcccagtga acacagtcgt gaaagctcgg gtccccaaga
     3001 gaaaaataag gaatgaaaag aacctctttc tttgcaaaca tttcctgaaa tttcatagga
     3061 tatcctttct gagtctctgc acttctgcca catccttgtc tccccgaccg gaaagacaga
     3121 tgatcatact cttctttttg ccgagttttt tcgcgatttt tttggcatag gcaaatgcgt
     3181 gggaagattc cagtgcggga atgatcccct ccagtttgca gacctcgaga aatgcatcca
     3241 aagcttcttc atccgtcaca ctcacatatt tgactctttt ggtttctgca agataggcat
     3301 gttcagggcc tacaccggga tagtccagtc ccgcagaaac ggagtgagcc ggtaccacct
     3361 gaccgtcttc atcctgaatc agaagagttt ttgtcccatg aaaaaaacca ggttttccga
     3421 aagtgattgt ggccgagtgt tctccaggag cagttcctcg tccccctgct tccacaccat
     3481 acagttctac tgacttgtcc ttcaggaatc cgtaaaacat tccaatcgca ttggaaccac
     3541 cgccgacaca ggcaaccacc gcatcaggga gttttttacg gacagccttg aactgtgcct
     3601 tggattcttt tcctatcaga ctctgaaaat cccgcacgat cgtcgggaat ggatgaggtc
     3661 cgatggctga acctacaata taatgagtat ttcctacatt caatgcccag tctctcattg
     3721 cttcactggt ggcatcctta agtgttgcag ttcctgcact cacaccaatt acctttgctc
     3781 cgagcatttt cattcgaaca gcgttcagtt cctgtcttct caaatcctct gttcccatat
     3841 acacaactgc ttctagtccg aacagagctc ctactgtggc agtggctact ccatgctgac
     3901 ctgcaccagt ttctgcaatg attcttttct ttttcatcgc aagggccaaa agtccctgcc
     3961 cgacggtatt gttgatcttg tgagctccag tatgattcaa atcttctctt ttcaggaata
     4021 tttccgcacc gccccagatt tttgtgagcc tttctgcata ggtcaggatc gaaggtcttc
     4081 ctatataatt cttctggtag tacaaaagct ttttattgaa atccttgtcc tttttcagct
     4141 tattgtaagt atcctcaagc tgaaagagtg cttctgtaag tatctccgga gcataccttc
     4201 ccccgaattc accgaaatat ccctggtttt tatcaaacgg actgacattc atattctaag
     4261 gacaaatttt catacaggaa aacaaatgca ataagcaaat ttcaagcaat gcagtcttcc
     4321 ttggcctatc ctgtcctttt gaaccaaatg ccgttcagac ctgcttcggt tgagtgctta
     4381 gcgacttcta ccgacaggaa taaataaact ctttccgact ctgtctgttc atctgcagaa
     4441 gaaggatgga cagtaaaaaa cagagtttct ggaaaaatat aagcatattc cgataatgca
     4501 gacttccctg ctgcctgaaa aatctgtctt gcctaaagaa caaaaaatca aaaagtagta
     4561 taaaataatt atttttaaat aagccgacag agaaactgaa gcatttctct gccgccgata
     4621 atccgcacta ggtgagttat ttattggaaa taatgtccaa tttaacagtt ctgtccggtg
     4681 tcagattaca gagttttcta tcggatgcgt agtctgcagt tataaaattc ccgaaatcac
     4741 agact
//
LOCUS       DAOQGJ010000058         4709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9571_length_4709_cov_3.394714, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000058 DAOQGJ010000000
VERSION     DAOQGJ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9571_length_4709_cov_3.394714"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK683_01550"
     CDS             <1..453
                     /locus_tag="PK683_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07157.1"
                     /translation="ETTPDYYRFKVELPASSEKVLKIVERQESQEKYDIFNFSADTVR
                     MFFNFAKPSEKAKEVLLKIAERYSEIDGLKLKMEDLRKEKEGISRRQNDVRKNIETFK
                     NADEQEYRQKFIQELMKTETRVSEIDSDLPAITKKIEEINRQILGMTF"
     gene            604..1632
                     /locus_tag="PK683_01555"
     CDS             604..1632
                     /locus_tag="PK683_01555"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH07158.1"
                     /translation="MINELMNIGTDFILNLPNNMLLFLSGRPQIEFDNKKLNPSLQLL
                     ASAVDMLPGFETLPVDQSRAMLEFLSEITFQKRSYSVKTEDIVILKGDSRMKARVYIP
                     KDGKSLKPVLVYYHGGGFVLGSIKSHDQVCRKISEAADCIVVSPEYRLAPEHTFPAAF
                     EDAAAAFSWTQDNIQKFSGNPKRAAVGGDSAGGSLAMAVTIALKEKIKKPYYSWLIYP
                     GTDFSRQRDSVKRYGKGLGLTKAMLDMFRDTYLPNEFDRFRYEASPVLADDFSGISSV
                     YVSIAGFDPLYDEDLALVKKLESAGIKTDYECYDSLIHGYISITAIPECRSAFENDIR
                     YLRKALHS"
     gene            complement(1605..2753)
                     /locus_tag="PK683_01560"
     CDS             complement(1605..2753)
                     /locus_tag="PK683_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07159.1"
                     /translation="MNRDIIQTIQKDLEEKMVFLSGPRQVGKTTLTYQILGAKTDSHP
                     AYLNWDFPKVRVPLMNGDIPADQKLIVLDEIHKYHDWRNLVKGLYDQYKSSIKFLITG
                     SARLDYYRRGGDSIQGRYHFHRLHPFSLTEAFPNPVKSDLELLLKFGGFPEPLFKGED
                     TFYRRWQLERQKLVLLEDMITLEKVREVSKVELLYSALTEKVGSPLSVNSMREDLSVA
                     HETVEHWLQIFENLYVIFRIPPMNSSKIRSVKKEKKMYFWDWSICRDSGPRFENLTAS
                     HLLRYCNYREDTYGYRMDLNYIRSSDGKEVDFVVLEDGNPKFAVECKLSEKEISPHIK
                     YFSERLDVPQFYQVHSGDEDYENSKFRTRVIPFIKFAQLLQEWSAFLK"
     assembly_gap    2797..2896
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3307..>4709)
                     /locus_tag="PK683_01565"
     CDS             complement(3307..>4709)
                     /locus_tag="PK683_01565"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNH07160.1"
                     /translation="SLSGAPGDFVKDIPIRELKFDIPEVRKEKLKEGLDYYGSVTEEF
                     PIAYLEVHIYGGESGTGNLPNETASLFCDVFSMGGSKSLPNEQLIAKIEAMGAHFSCS
                     SEYEKIILSVSYLTRDQDEVMNLIEDLWKNPAFSDNALVSGKKKMKEAIKRRNEKTDS
                     LGFRKAREWVFRSFKKGIVPQSESVDRISRDTLSTFFENSVRKNRKAIIASGRFDQDK
                     LKAWLKDLFIENANTGSDISESSSENVLPSEITLKKLKEDFAADSKNILLVQKDVNQS
                     FLLKIGVAPEHSHPDFFPIQLLNFILGDGFNSYLMTEIRVKRGLAYSAHSRPLFEKDY
                     GLLYFYTATKNETLGETKKIMEGILTDEVYSKITEEELQTAKNSIISQFVFLFTNQRV
                     ILENQLNFDEDNMPSDYLKNYRKRMQAVTLDDIRRVGKKYFLGENMKTVIVTNQATAE
                     KVLPNVKPIAPEDKVK"
BASE COUNT         1354 a          950 c          902 g         1403 t
ORIGIN      
        1 gaaactacgc cggattatta cagattcaag gtggaactgc cagcatcatc tgaaaaagtc
       61 ttgaagattg tggaaagaca ggaatcacag gaaaagtatg atatcttcaa tttttctgcg
      121 gatacagtgc gaatgttttt taattttgcg aaaccgagtg aaaaagccaa ggaagtgctt
      181 ctgaaaattg cagagaggta ttctgaaatt gacggactta agctgaaaat ggaagatctc
      241 agaaaggaaa aagaagggat ttccagaaga cagaatgatg tccgaaaaaa tatcgaaaca
      301 ttcaaaaatg cagatgagca ggagtacaga cagaaattta ttcaggaact gatgaaaact
      361 gaaacgagag tatcagaaat tgattcagat ctgccggcaa ttacaaagaa gattgaagaa
      421 ataaacagac agatacttgg aatgacgttt tgaacagatc taagcagaca ctttttttct
      481 gctggactgt ataagcgtat tttaatatat atgaagcata cggaaaatac ttaaactgct
      541 ttggtcaggt gcagaatcat ttctgccccc aaagcattta tttttagata gactgagcaa
      601 aaaatgatta atgaactgat gaatatcggc actgatttta tactgaatct gcctaacaat
      661 atgctcctgt tcctttcagg cagaccgcag atcgaatttg acaataaaaa actgaatcca
      721 tctcttcagc tccttgcctc cgcagttgat atgcttcccg gatttgaaac tctccctgtt
      781 gatcaatcca gggcaatgct ggaatttctt tccgaaatca ctttccaaaa aagatcttac
      841 tcagtcaaaa cggaagacat tgtgatcctg aagggcgaca gcagaatgaa agccagggtc
      901 tatattccga aagacggaaa gagcctgaaa cctgtacttg tatattatca cggcggcgga
      961 tttgttctgg gaagcatcaa atctcatgat caggtatgca ggaaaatttc agaagcagcc
     1021 gactgcattg ttgtttctcc tgaatacaga ctggctcccg aacacacatt tcctgccgcc
     1081 tttgaggatg cagccgctgc cttctcctgg acacaggaca atatacagaa gttttccggg
     1141 aatcccaaaa gagcagcagt cggaggagac agtgccggcg gaagtcttgc tatggcagta
     1201 accatagcgc tgaaggaaaa aataaagaaa ccatattata gttggcttat atatccaggt
     1261 acagattttt ccagacagag ggattcagtg aaaagatacg gaaagggtct tgggctgaca
     1321 aaagcaatgc tggatatgtt cagagacact tatctgccga atgaatttga cagattcaga
     1381 tatgaagcat ctcctgttct tgctgatgat ttttcaggaa tctcatctgt atatgtttcc
     1441 atcgcaggat ttgatcctct gtatgacgaa gatcttgcat tggttaagaa actggaaagt
     1501 gcaggtatta agacagatta tgaatgttat gacagtctga ttcatggata tatctccatt
     1561 actgccatac cggaatgcag atccgctttc gagaatgata tccgttactt gagaaaagca
     1621 ctccattcct gaagaagctg tgcaaattta atgaacggaa tgactcttgt tctgaatttg
     1681 gaattctcat aatcttcatc acctgaatgc acttggtaga actgagggac atccagtctt
     1741 tctgaaaaat attttatatg tggacttatt tctttttcgg aaagtttgca ttccacggca
     1801 aacttgggat ttccatcttc caaaacaaca aaatctactt cttttccatc agaacttcgg
     1861 atataattta aatccattct gtatccgtat gtatcttctc tgtaattgca gtaccttaaa
     1921 agatgagatg cagtcagatt ttcaaatctt ggaccagaat ctctgcagat agaccagtcc
     1981 cagaaataca ttttcttttc tttctttact gatctgattt tggaggaatt cattggagga
     2041 atcctgaaaa tgacatacag gttttcaaat atctgcagcc agtgctcgac agtttcgtgt
     2101 gcaacggaaa gatcttctct cattgaattt acagaaagcg gtgaccctac tttctcagtc
     2161 agtgctgagt aaagcagttc aactttggaa acctctctga ctttctccag agtaatcata
     2221 tcttccagaa gcacaagttt ctgccgttcc agctgccatc gccgataaaa tgtatcctct
     2281 cccttaaaca atggttcagg aaaacccccg aatttcagga gcagttccag atcagatttg
     2341 accggattgg gaaaagcttc cgtcagactg aaaggatgaa gtctgtgaaa atggtatctt
     2401 ccttgaatgg aatctccgcc tctcctgtaa tagtccagtc ttgcagatcc tgtaattaga
     2461 aatttaattg aggatttata ctgatcgtaa agccccttta ccagattccg ccaatcgtgg
     2521 tacttatgaa tttcatccaa gacaatcagt ttctgatcag caggaatgtc cccattcatc
     2581 aagggaaccc ttacctttgg gaagtcccag tttaaatagg caggatggga atcagttttt
     2641 gctcctaaaa tttggtaagt cagggttgtt tttcccacct gtctcggccc agaaaggaag
     2701 accattttct cttccaaatc tttctgaatt gtttgaatta tgtctctatt cattgtaatt
     2761 tccagtggac tcgaaaatac tcaagtctaa tttcagnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnngtct ctattcattg taatttccag tggactcgaa aatactcaag
     2941 tctaatttca ggtagactcg aaaatactca agtctaattt caggtagact cgaaaatact
     3001 caaattgatc cggtttaatg ggcaattcaa ctgaagaatc gtttttctac caaggtttaa
     3061 tatttcgtga atagttctta cttacttttt gaaagtcacc gtatattgac gatggaaaac
     3121 agcggcgatc gaataaacgg tttcttactg catttagctg tatttattct tcttctggaa
     3181 ggaaattctc tctgcggaat gctttaagat ttctccttcc ttcggtcgtc gaaatgacag
     3241 cagacctgcg gggaacgata tacgatcccc aaagatataa ctcagtaaac atttctctag
     3301 ttaattctat ttcactttgt cttccggagc aatcggcttt acattcggga gaactttctc
     3361 agccgtagcc tgattggtta caatcacggt tttcatattc tctcccaaaa agtatttttt
     3421 cccgactctt ctgatatcat ccagtgtgac tgcctgcatt ctttttctgt aatttttcag
     3481 ataatctgaa ggcatattat cttcatcaaa gttcagctga ttctccagaa tgactctctg
     3541 attagtaaac agaaatacaa actgactgat aatcgaattc tttgcagtct gaagttcctc
     3601 ctctgtgatc tttgaataaa cctcatctgt gagaattcct tccataatct tttttgtctc
     3661 accaagagtc tcatttttag ttgcagtata aaaatagaga agaccgtagt ctttttcaaa
     3721 gagcggtctg ctgtgagccg agtaggcaag acctcttttg actctgatct ccgtcatcag
     3781 ataactgttg aatccgtctc ccagaataaa attcagaagc tggatcggga aaaagtcagg
     3841 atgactgtgt tccggcgcca ctccaatctt cagaagaaac gactgattta catccttctg
     3901 aacaagaagg atattttttg aatcagcggc aaagtcctct ttaagctttt ttaaagtaat
     3961 ctcagaagga agtacgtttt cagatgagga ttcagagata tctgagcctg tatttgcatt
     4021 ttcgataaac aggtctttca gccaagcctt cagtttatcc tgatcaaatc taccgctcgc
     4081 gataatcgct tttcgatttt ttctgactga gttttcaaaa aatgtactga gtgtatcccg
     4141 gctgattctg tctacgcttt ctgactgagg cacaatccct tttttgaaac ttctgaaaac
     4201 ccattccctt gcctttctga acccaagaga atcagtcttt tcattccttc ttttgatggc
     4261 ttccttcatt ttctttttcc cgctgacaag tgcattgtct gaaaatgcag gatttttcca
     4321 aagatcttca atcagattca tcacttcatc ctggtctcta gtcagatagg aaacagagag
     4381 gataattttt tcatattcgg aagaacagga aaaatgcgct cccattgctt cgattttggc
     4441 aatcagctgt tcattcggca gagactttga tccgcccata ctgaagacat cgcagaaaag
     4501 ggaggcagtt tcattcggaa gatttcctgt tccggattct cctccgtaaa tatgcacttc
     4561 cagataggca atcggaaatt cttccgttac gcttccgtaa tagtccagtc cctctttgag
     4621 tttttccttc ctgacttcag gaatgtcaaa tttcagctca cggatgggaa tgtcctttac
     4681 gaaatcaccg ggggctcccg agagagatg
//
LOCUS       DAOQGJ010000059         4699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9598_length_4699_cov_2.929371, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000059 DAOQGJ010000000
VERSION     DAOQGJ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9598_length_4699_cov_2.929371"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PK683_01570"
     CDS             <1..665
                     /locus_tag="PK683_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07161.1"
                     /translation="KIKTAEEDAKELKKRMRRVENFLEIDPDLDAYNNNPRNMMSVVW
                     RSALIPGWGLNYAKSNFGNIYMTAFLVTGLAAVFWNENLKAMEKDLKNKAFNDFLVQP
                     ITAQSVVEATASTGTPSYLTDSFTQLQSSNLTTKLIKYNQSKQDYEVQKQRGQSLLGI
                     AAGIYAVQLLHSALEGYFWAKKTAPGKFDEKKTGFNWNITPSRTVYGQNWNFDLKYEW
                     EF"
     gene            complement(957..1661)
                     /locus_tag="PK683_01575"
     CDS             complement(957..1661)
                     /locus_tag="PK683_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH07162.1"
                     /translation="MVSYIIKFFLIFISIMTSACSDINKGEGNRDQSLTFFDRPEKDR
                     LPYFRGKDLEAFWLKPDQKLTEARKVDPFKFTNQLNAEFGLDSLKNKITAVSFFFAKC
                     NGICPNITRNLIFVQSSYKDNPNVQLVSFSVTPDLDTPEALRKFADEKGIISGKWNLL
                     TGPRNRIHSMARTVFQADTNTINKNPNDFVHSEQIFLIDKNLYFRGVYNGNKGDSVQT
                     MISDIKILENETSVSN"
     gene            complement(1675..2235)
                     /locus_tag="PK683_01580"
     CDS             complement(1675..2235)
                     /locus_tag="PK683_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-binding protein"
                     /protein_id="HNH07163.1"
                     /translation="MNKKFFLLLLSLLLIHCGPEIHMDNDPNFTLGYESVFSYKGVPY
                     TGIMLSVHPNGVRRETEYKDGLEHGKTSDTYADGRPAAEYFFEKGKRIGIHKGWYENG
                     KPRFWYEYKDGMAHGDHWEWHEEGIPYRYGRFENNVNIGQKVWRKDGQIYSNYIYTEQ
                     RIYGVQGSKLCFKLKGDETNTRTVVK"
     gene            complement(2302..3183)
                     /locus_tag="PK683_01585"
     CDS             complement(2302..3183)
                     /locus_tag="PK683_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNH07164.1"
                     /translation="MKSAKMTTLLIGVLLMQCSSKKKDNKSAAALALLAASSSSSTAA
                     YSASCTTTFGTGVPDWIKNSFTCVTVSVSGSNYVFKTNSLPTYKSVYWGSSSAGYETA
                     MYTNTTGTNKANPNTIKSQNITITVPMDNTSTTAPATGYGAIGVATNGIAIYNDQAAP
                     GDSLSTEYYTFDSSQGHPTNTGAYHYHVEPPKISVSDSKLVGIALDGYLIFGKLHDTT
                     SSGLTTGFTLGGSSSSTQCTGSGLTTDVPTTWSQYTNYTSQKTAGTRRHYHVNNGSEV
                     NGILLSGKYIGAAGTSQ"
     gene            3530..3955
                     /locus_tag="PK683_01590"
     CDS             3530..3955
                     /locus_tag="PK683_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07165.1"
                     /translation="MFCYFKSNYKTFLKSVMLKIGRISARFHISFRSFLILFFIFQVL
                     MLPEAEAQLKQEMNWDTDSSSARMTGRSAALYCSRKGKRLPDMRELADAIHLFSNSSG
                     HWTSYTYPGGSRVFCAYLKSGPVCSDQKENRKAYARCVK"
     gene            4017..>4699
                     /locus_tag="PK683_01595"
     CDS             4017..>4699
                     /locus_tag="PK683_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07166.1"
                     /translation="MKEQISIPMIRGEKIVNQIRFGLSVFYIITILSTVSLVTWPMTA
                     AYIFCVFLTLLYTAVSYQAIKEDRLTHSMIYWASAFDILIMTGLQILQIFLEPAGVDY
                     TVKDKLFYTFMFFYVVLTPIRFDSRFAVFNGIGISAGLASVYITAAFRGMDFTMNMSE
                     SLTKNHYPLSSIISSYLAFAVANYMAYILSRLTNSAMKDLIEKENLARNVLEKNSTIV
                     GNLKDSTEKL"
BASE COUNT         1276 a          955 c         1042 g         1426 t
ORIGIN      
        1 ccaaaatcaa gactgcagaa gaagatgcaa aagagctgaa gaaaagaatg agaagggtag
       61 aaaattttct ggaaattgat cctgatctgg atgcatacaa caacaacccg agaaatatga
      121 tgagtgtcgt gtggagatcc gcactgattc ctggatgggg cctcaattat gcaaaaagca
      181 attttggaaa tatctatatg actgcatttt tagtcactgg acttgccgca gtgttctgga
      241 atgaaaatct gaaagcgatg gaaaaagatc tgaaaaacaa ggcattcaat gattttcttg
      301 tgcaaccgat cactgcacag agcgttgtgg aagccactgc atcgacaggc acaccgtcct
      361 atctgacaga ctcatttact cagcttcagt cttccaatct gacaactaaa ctgatcaagt
      421 ataatcagtc caagcaggac tatgaggtac agaaacagag aggacagagt cttctgggaa
      481 ttgctgcagg gatttatgcc gtccagcttc tgcattctgc gctggaagga tatttctggg
      541 caaagaagac tgctccggga aaatttgatg aaaagaaaac agggttcaat tggaatataa
      601 caccttccag aacagtgtac gggcagaact ggaattttga tctgaagtat gaatgggaat
      661 tctgaaatat ttcggtttct tattgatact cctttatcat tgtgaccttt atcatcctgc
      721 acggaatctt cagtgtcctg gtgcaaaatc cgattttgga tgcccgggat ctgatgaatt
      781 caggaaaaaa aagaaaagct gtattgagta taatctgctt atggcagata ttttcgggca
      841 atgttcaaat ggattcggag actgtaaaaa cccttattat ctgaatcttc ggattatgtg
      901 tgcaatgggc tgtcccggaa cagaagcttg tatgtgatgt cagaagacag gagttttcag
      961 ttgctgacgg aggtttcatt ttccagtatt tttatgtcgc tgatcatcgt ctgaaccgag
     1021 tctcctttgt ttccattata aactcctcta aagtaaagat ttttatcaat cagaaaaatc
     1081 tgttcagaat gaacaaagtc gttgggattt ttgtttatcg tgttggtgtc tgcctgaaag
     1141 acagttctgg ccattgagtg aattctgttc cgaggtccgg taagaagatt ccattttccg
     1201 ctgataattc ccttttcatc ggcaaatttt ctcaatgcct caggagtgtc caggtcagga
     1261 gtcactgaaa aagaaacaag ctgaacattc ggattgtcct tataggaact ttgtacaaaa
     1321 ataagatttc tggtaatgtt cgggcagatc ccgttgcatt ttgcaaaaaa gaaagaaaca
     1381 gccgttattt tgtttttcag actgtctagt ccaaattcag cattcagctg attcgtaaat
     1441 ttgaacggat ccaccttccg ggcttcagtg agtttctgat ccggcttgag ccagaaggct
     1501 tccagatcct tgcctctgaa atagggaaga cggtcttttt caggtctgtc gaaaaaggtg
     1561 agactctgat ctctgttacc ttcccctttg tttatatcag agcaggccga agtcattatt
     1621 gaaatgaaaa taagaaagaa ctttattata taggatacca ttattttctc cgccttactt
     1681 gacaactgtt cttgtgtttg tttcatcacc tttgagcttg aagcagagtt ttgatccctg
     1741 aactccgtaa attctctgct cagtatagat ataatttgaa taaatctgcc cgtcctttct
     1801 ccagactttc tgcccgatat ttacattgtt ttcaaatcgt ccgtacctgt aagggatgcc
     1861 ttcctcatgc cattcccaat ggtccccgtg agccattccg tctttgtact cgtaccagaa
     1921 tctgggcttt ccgttttcat accagccctt atgaatcccg atgcgttttc ctttttcaaa
     1981 aaaatattct gcagcaggtc ttccgtcggc atatgtgtct gaagttttcc cgtgttccag
     2041 cccgtccttg tattcagttt ctcttcgaac accgtttgga tgaactgaca gcattattcc
     2101 tgtataagga acacctttat aggagaagac actttcgtac cctagcgtga aattcggatc
     2161 attgtccata tgaatttcgg gtccgcagtg aatcagcagc aaggataata ataaaagaaa
     2221 aaattttttg ttcataagaa tctgttctgt gaggaagcct gtcagtccga caggccgttt
     2281 ttcgtcagcg gtatctcccc tctattgaga agttccagca gcgccgatat actttcctga
     2341 gagaagaatt ccgtttacct cggaaccgtt gtttacatga taatggcgtc ttgtgcctgc
     2401 agtcttctgg ctggtgtagt ttgtgtactg ggaccaggtt gtgggtacat ccgtcgtaag
     2461 tcctgaacct gtacactgag tgctgcttga ggatcctcca agtgtgaatc ctgtcgtaag
     2521 tccggaagag gttgtatcat gaagtttgcc gaatatcaga taaccgtcca gtgcgattcc
     2581 tacaagtttg ctgtcgctta ctgagatttt aggaggttcc acatgatagt gataggctcc
     2641 tgtatttgtg ggatgtccct gcgaagaatc aaaggtataa tattcagtgg acagtgaatc
     2701 tcccggggcc gcctgatcat tgtatattgc aattccgttc gtcgctacac cgattgcacc
     2761 gtatcctgta gcaggtgcag tcgtcgatgt attgtccata ggtacggtga ttgtgatatt
     2821 ctgggatttg attgtatttg gatttgcctt gtttgttccg gtcgtgttgg tatacattgc
     2881 tgtctcatac cctgcactgg aactgcccca gtaaacactt ttgtaagtcg ggagagaatt
     2941 tgtcttgaaa acataattgg aaccgctgac tgaaactgtg acacaggtga agctgttctt
     3001 tatccagtcc ggcactccgg tgccgaacgt tgttgtgcag gatgctgaat aggctgccgt
     3061 tgaggaagat gatgatgcgg caagaagagc aagtgccgca gcggatttat tgtctttttt
     3121 cttggaactg cattgcatca gcagaacacc gatcagtaag gttgtcattt tggcagattt
     3181 cataggatgc tattgaaatt cagtctctgt attattcaaa cacaaaaatt gtctcatatt
     3241 aaaaatactc agatttccaa tgaaatttta atctggtcga ttattcattg attgaaagtg
     3301 cctttgcatt ttcactgaac gtgagcaaat gaggggattt caaaggggcg gcttaagcct
     3361 ctttaaccca acgcaagttt ggaagctgct gccgacaggc atacctgccg tttttgattt
     3421 ctaatagccc gcagggcgga gcgcagcgag attagaaatc tgggaaatat attgatccag
     3481 tcagccggac ttcattacat caggactgcc gccaatcagg agagaaaata tgttctgtta
     3541 ttttaaatcg aattataaaa catttctaaa atcagtgatg ctgaagatcg gcaggatctc
     3601 tgcccgtttt catatttcat tcaggtcttt cctgattctg tttttcattt ttcaagttct
     3661 gatgcttccg gaagcagaag ctcagctcaa acaggaaatg aactgggata ctgattcgag
     3721 cagtgccaga atgaccggca gatcggcagc tctctactgc agcagaaagg ggaaaagact
     3781 tccggatatg agagagcttg cagacgcaat tcatctgttc agcaattctt caggtcactg
     3841 gacatcctat acttatcccg gcggatcaag agtattctgc gcatatctca aatccggtcc
     3901 ggtctgttcc gatcagaaag agaacagaaa ggcatatgca agatgcgtga agtagggata
     3961 tttctgcagg gaaattattt gcaaaaacgg actgctgaaa atcagatgta gattatatga
     4021 aagagcaaat ttccataccg atgatccggg gcgaaaaaat tgtgaaccag atacgcttcg
     4081 gtctctctgt gttttatatt attacaattc tttcaacagt cagtcttgtt acctggccga
     4141 tgactgctgc ctatatattc tgtgtatttc tgacactgct ttatactgct gtatcctacc
     4201 aggcaatcaa ggaagacagg ctcactcaca gtatgatcta ctgggcttct gcatttgata
     4261 ttctgattat gacaggcctt cagattcttc agatttttct tgaaccggca ggcgtggact
     4321 atacagtaaa agataagctt ttttatacat ttatgttttt ttacgttgtt ctcactccga
     4381 tccggtttga cagcagattt gcagtattca acgggatagg aatttcagcg gggcttgctt
     4441 cagtctacat cacggctgca ttcaggggta tggattttac gatgaatatg tcggagtctc
     4501 tgacaaaaaa tcattatcct ctgtccagca tcatcagttc ctatcttgcc tttgctgtag
     4561 ccaattatat ggcttacatc ctttcccgtc tgacaaattc tgcaatgaag gatctgattg
     4621 aaaaggaaaa ccttgcaagg aatgttctcg aaaagaacag cacaattgtg ggcaatctta
     4681 aagattcgac agaaaaact
//
LOCUS       DAOQGJ010000060         4620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_9844_length_4620_cov_3.519606, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000060 DAOQGJ010000000
VERSION     DAOQGJ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9844_length_4620_cov_3.519606"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PK683_01600"
     CDS             complement(<1..146)
                     /locus_tag="PK683_01600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388290.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNH07167.1"
                     /translation="MQIYQKTTNKYQGKFKDLPEGHLLVNLGPSHPATHGILQNIVQL
                     DGER"
     gene            complement(148..666)
                     /locus_tag="PK683_01605"
     CDS             complement(148..666)
                     /locus_tag="PK683_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNH07168.1"
                     /translation="MKEKVQSYLDKELSQFIYKKEETKSNIPTFFIKPEGIIPVAKGL
                     KENPELKFDFLNDLTAIDWLGKKNPRFEVCYLLRSNSNGFARIMLKCPLEEGEKIPSL
                     VKVFKGANWPEREVFDLFGIEFIGHPYMERIVLPDNFQGHPLRKDYPLEGFGQDYLIA
                     NLLHIHQEDKGE"
     gene            complement(663..1175)
                     /locus_tag="PK683_01610"
     CDS             complement(663..1175)
                     /locus_tag="PK683_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002083574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNH07169.1"
                     /translation="MVQVTHLDTFLNWGKSYSLWPYPFATACCGIEFMGTACSDNDIA
                     RFGSERPSFSPRQADMILVLGTITYKMAPVLREIYDQLSEPKYVISVGACASSGGMFD
                     TYGVVQGIDRFLPVDIYIPGCPPRPEAIMDALVKLQQKVKTQGLEDRRQEVMRKIDEI
                     NERNKPLVVK"
     gene            complement(1214..1591)
                     /locus_tag="PK683_01615"
     CDS             complement(1214..1591)
                     /locus_tag="PK683_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNH07170.1"
                     /translation="MGSTPEGFGPIVVQLLLALGFSVLILSLALLISPRKKSKPHDTF
                     ECGVPYYGDARGIFNIKFYLVAVLFILFDIEAVFLYPWAVNLGKFNEAGLGGFLMIEM
                     LVFIFILVVGLYYIWKKGALEWD"
     gene            1798..3534
                     /locus_tag="PK683_01620"
     CDS             1798..3534
                     /locus_tag="PK683_01620"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07171.1"
                     /translation="MIIPYGDANFESVVTKKALLIDKTKYIPLLEKHSKFFLIRPRRF
                     GKSFMLSLLQSYYDIKTKDKFDTLFGGLEIHKSPTELRNTYIILKMDFSGLNADTDEI
                     LKREFTNKVISYIDFSLSFYAELFPSNIYSAYHDFLRNREIEPSDALLFLIGAVKKTP
                     YKVYVLIDEYDNFINNLVSLDREGFAKRILSRSGFVRSFYETLKIGSGEGAFDKFFIT
                     GVSPLMLDELASGFNIMEDMTQKPAFASMLGFTEEEVQSVLDLIPPDVYSQTDKTNDE
                     VMKDLTHYYNGYRFSPLNPARVFNPDMVLYFIQDFQDRMYPMDLLDLNVKTDYSKLKG
                     LIISLSGRDMLKKIIEELSSAEETGVDLVRRFTFEQRFSENELKSLLYYLGLLTYTDR
                     RSRLKVPNYVIHVLFWEYLRSLLQEESKVDFDFSLLQNTLDDLAFKGSFVSLEILLKD
                     FFENRLSNMDYSRFSEKHVKIMLISYLSMSRMYNIISEREIPKGRRIDLLLESHPTYD
                     EVRYNYVIELKYIKKEMFSEADRIKSEAVEKVKEDRRIYEESFRQRGRTVLSAVILVF
                     SDKRAELIAVRK"
     gene            complement(3716..4489)
                     /locus_tag="PK683_01625"
     CDS             complement(3716..4489)
                     /locus_tag="PK683_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07172.1"
                     /translation="MKQYIFSILFLSAFSQNCYVDNPRKECEENANVLNFQAAYSFGK
                     ACLYPTSDNDPNSPLRCLAVALYNQSCRKKAETVYKADFGIDGSRQGKKENETNQRFG
                     ILALILLSEIKENGYVYYKGRNLLATLEYLAGQHIAGNAQNGETKTVSCSKSGTANTV
                     FFKEEWVISFQQCQHSTAFSGDASDVTWNGNFQGTLTYTKYSAALFLNRVSMFGTLKY
                     SGNIFWTLFGSRNIDETCSVSAEYSSAPGKYIFCGRPSY"
BASE COUNT         1341 a          975 c          950 g         1354 t
ORIGIN      
        1 attctctctc cgtccagctg aacgatgttc tgcagaattc cgtgtgtggc aggatgactt
       61 ggtcccaggt tgaccagaag atgtccttcc ggaagatctt tgaatttacc ctggtattta
      121 ttcgttgttt tctggtaaat ctgcatctta ttctcccttg tcttcctgat gaatgtgcag
      181 aagatttgca atcagataat cctgaccgaa tccttccaaa ggatagtcct tgcgaagagg
      241 atggccttga aaattgtccg gaagaacgat tctttccata tagggatgac cgatgaactc
      301 aatcccgaac agatcaaaaa cctctctttc cggccagttg gcacccttga ataccttcac
      361 aaggcttgga attttctcac cttcttccag agggcatttc agcataattc ttgcaaaacc
      421 gttggaattg cttctaagaa gataacatac ttcaaaacgg ggattctttt tgccgagcca
      481 gtcaattgca gtgaggtcat tcagaaaatc aaacttcagt tcagggttct ctttcagtcc
      541 tttggcgaca ggaatgatac cttccggttt gatgaagaaa gtggggatat tggacttagt
      601 ttcttctttt ttataaataa actgactgag ctctttgtct aaatagctct gaactttttc
      661 tttcatttta ccaccagagg cttatttctt tcgttgattt catcaatctt tctcataact
      721 tcctgtcttc tgtcttcgag cccctgagtt ttcactttct gctgaagttt gacaagagcg
      781 tccataattg cttccggacg aggaggacat ccaggaatat aaatatccac tggaagaaat
      841 ctgtctatcc cctgaacaac cccgtatgta tcaaacattc ctccggaaga ggcacatgca
      901 cctacgctga tcacatactt aggttctgaa agctgatcat agatttctct gagaactgga
      961 gccattttat atgtaatggt cccaagtacc agaatcatat cggcctgtct gggcgaaaaa
     1021 gaaggtcttt ccgatccgaa gcgggcaata tcgttgtcgg aacaggctgt tcccataaac
     1081 tcaatcccgc agcaggcagt ggcaaacgga tacggccaga gggaataact ctttccccag
     1141 ttaagaaatg tgtccagatg agtcacctga accatatccc cgatgacatc acctgttttt
     1201 tgcataatgt cgcttaatcc cattcgagtg ctcctttttt ccagatataa tacaatccga
     1261 ctacaaggat aaagataaat acgagcattt ctatcatcag aaacccgcct aagccggcct
     1321 cattgaattt tcctaaattg accgcccaag ggtataaaaa cacggcttca atatcaaaaa
     1381 gaatgaacaa aactgccaca agatagaact taatattgaa aattccccta gcatcgccat
     1441 aataaggaac accgcattca aatgtgtcat ggggcttgct ttttttacga ggactgatca
     1501 gtagggcaag gcttaaaatt aagacagaaa aacctaatgc caagagtaat tgtacaacaa
     1561 tagggccaaa gccttccggt gtgcttccca ttcgacctcc gatttcactc actattttta
     1621 gtaagaggag cctatgtcaa acaaattctt ttttaggatg gaaaaataag tttggggttc
     1681 aggggtcact agggacacac agctgagtgt ccctgcgaac accatatgtc cctgcaggtt
     1741 cagatcagta actcctttct tcttgacacc aagttcttgc taattcagct tagacgtatg
     1801 atcattccct acggcgatgc aaactttgag agtgtagtta ccaaaaaagc tctcttaatt
     1861 gacaagacaa aatacatccc tcttctggaa aaacacagca agtttttcct tatccgcccc
     1921 cgccggtttg ggaaaagttt tatgctgagc cttctgcaga gttactacga catcaaaacc
     1981 aaggacaaat tcgacacgct tttcgggggt ctggagatcc ataaaagtcc gacggaactg
     2041 aggaacactt acattatcct gaagatggat ttttccggac tcaatgcaga tacggatgag
     2101 atactgaaga gagaatttac gaacaaagtg ataagttata tagacttttc tttgagcttt
     2161 tatgcagaac tttttccatc aaatatctat tctgcctacc atgattttct gcgcaacaga
     2221 gagattgaac cttcggatgc acttctgttt ttaataggag ccgtgaaaaa aactccttac
     2281 aaagtctatg tgcttattga tgaatatgat aattttataa acaatctagt ttccctggac
     2341 agagaaggtt ttgcgaaacg gattctttcc cgaagcgggt tcgtgagaag tttttatgaa
     2401 acactaaaga taggaagcgg ggaaggtgcg tttgacaagt ttttcattac aggtgtttcg
     2461 cctctgatgc tggatgagct tgcgagcgga ttcaatatta tggaggatat gacacagaag
     2521 cctgcttttg catcaatgct gggtttcacg gaagaagaag tgcagtcggt acttgacctg
     2581 attccgcctg acgtctattc tcagacagac aaaacaaatg atgaggttat gaaagatctg
     2641 actcattatt ataatggtta cagattttct cctttaaatc ccgcaagggt tttcaatcct
     2701 gatatggttc tgtatttcat tcaggatttt caagacagaa tgtatcctat ggatcttctg
     2761 gatctgaatg ttaagacaga ctacagcaaa ctgaaggggc tgatcatcag tctttccggc
     2821 agggatatgc tgaaaaaaat cattgaggaa ctgtcctctg ctgaagaaac tggagtggat
     2881 ctcgtccgtc gttttacatt cgagcagaga ttcagtgaaa acgagctcaa gtcccttctc
     2941 tattacctgg gacttctgac ttacacagac agacgtagca gactgaaggt tcccaactat
     3001 gtaattcatg ttttgttctg ggaatatctg aggagtctgc ttcaggaaga gtccaaggtg
     3061 gattttgatt tttctctgct ccagaatact ttggatgatc ttgccttcaa gggcagtttt
     3121 gtttcacttg aaattctgtt gaaggacttt ttcgagaaca gactttccaa tatggattac
     3181 agccggttca gtgaaaaaca cgtgaagatt atgctcatct cttatctttc tatgagccgg
     3241 atgtacaata tcatttccga aagggaaatc cccaagggca ggagaattga tcttcttctc
     3301 gaaagccatc cgacatacga tgaagtacga tataattatg tgatagaact gaaatatata
     3361 aagaaggaaa tgttctccga ggcagacaga atcaagtctg aggcagtgga aaaagtaaaa
     3421 gaggacagac gcatctacga agaaagtttc cggcaaagag gcagaactgt gctttcagct
     3481 gtgattcttg tattctcgga caagagggca gagctcattg cagttcgcaa atgaagatct
     3541 gccgatggat tttctgaatc agtatttttc tgcaagacat cagattcgca gttcaatttc
     3601 cgaaattgaa aaacagagaa catgaaattc aagaaattgc agctttctgc acagtgtaca
     3661 ctattacagt atttgttaaa aaaagacttt ggaaggttta agtcatcagg cagtatcagt
     3721 acgatggtct gccgcagaaa atatatttcc cgggtgcgga actgtattcg gctgaaacag
     3781 agcaggtctc atcaatgttc ctgctgccga acagtgtcca gaaaatattt ccggaatact
     3841 tcaatgtgcc gaacatggaa accctgttca gaaataacgc tgctgaatat tttgtgtaag
     3901 tcagagttcc ctgaaaattt ccgttccaag ttacgtctga tgcatctccg gaaaaggctg
     3961 tgctgtgctg acactgctgg aaactgatca cccattcctc cttgaaaaaa actgtattgg
     4021 ctgttccaga tttactgcag ctgacagttt tagtttctcc attctgcgca ttgcctgcaa
     4081 tatgctgtcc tgccaaatat tccaatgtcg ctagaagatt tctgccttta tagtatacat
     4141 acccgttctc cttgatctcg ctcagaagaa taagtgccaa tatgccgaat ctttgattgg
     4201 tttcattttc ttttttccct tgccgggaac catcaattcc aaaatccgct ttataaacgg
     4261 tctccgcttt ttttctgcag gactgattgt acagagccac tgcaagacat cggaggggac
     4321 tgttcgggtc gttgtccgat gtcggataaa gacaggcctt tccgaaggag tatgcggcct
     4381 gaaaattcag tacatttgca ttttcttcac actcctttct aggattgtcc acatagcagt
     4441 tttggctgaa cgcagaaaga aataaaatag agaagatata ctgtttcatc tgaaaatatc
     4501 ctttaagcaa atattgactg cttcggaaaa cgtaactcaa aatcagacct tggcaaatat
     4561 catataatga cttcaaaaaa atgacttgca tatctgtaaa tggcactata taattaattc
//
LOCUS       DAOQGJ010000061         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10009_length_4576_cov_2.759788, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000061 DAOQGJ010000000
VERSION     DAOQGJ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10009_length_4576_cov_2.759788"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PK683_01630"
     CDS             complement(<1..765)
                     /locus_tag="PK683_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNH07173.1"
                     /translation="MQHRGQESSGIVSTDGEHIYRYAGMGLVASVFNQTTLKELCGSA
                     AVGHNRYSTTGASFLRNAQPLRVESNLGPLALAHNGNLVNSYEIRRKMEAEGSIFQTT
                     IDTEIIVHLMAKSGEKDLLTAFSNALRQVDGAYSLLLLTEKKLIAVRDPNGFRPLVMG
                     KRPDGAIVFASETCAFDITDTEYVRDIEPGEMVVIDQFGEKSYFPFGKRKKSLCIFEY
                     IYFSRPDSNIFGQSVYKVRKHLGALLAKVKPVDADVV"
     gene            947..2161
                     /locus_tag="PK683_01635"
     CDS             947..2161
                     /locus_tag="PK683_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNH07174.1"
                     /translation="MQTKSNYTLITNKRGLELAAVSFNNSRQIAIDTESSGYYTYVPR
                     VCLIQITCTGKNYIIDPLSGIDLRILGDIFENTKVLKIFHSAIDDIKALKRDFSFKFK
                     NIADTMYSSKLLALEHNSLNFLAEYYLKIKLSKTEQKSNWEKRPLDRSQLEYASLDTA
                     YLETIWAKMNTELEAQGLLEEAESEFSKFCEEVYKPRETEEIAWHKFPNVMKFTPEQR
                     RTVHDILQYREEKAKRLNKASFRIVNNEIIKKIATEKHTEESLLALLGKKEGKDVFDL
                     LQNPSGPPLEKKDIPKLDENMTPAEEVKLRHFHKWRDKIMKKRNIDHSMLLSNRQFLQ
                     ILRTKPADLEALEKLEILSGWKVKNYGPSFLKMLKGEPYDDLITHLKPIGAKVEIRES
                     KTEIIQTDSAQP"
     gene            2106..2726
                     /locus_tag="PK683_01640"
     CDS             2106..2726
                     /locus_tag="PK683_01640"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNH07175.1"
                     /translation="MKFENQKLKLFRPTPLSHSIHNSGARDSAVIIPIFEKEGRDLIL
                     LTRRSPHLKSHPGQISFPGGVVEKGESFQDTARREWEEELGSSSDTLEIISEFQPMHT
                     FTGFIIHPIIARYRGDSSFSPNENEVSEILEFDPEDFYSKRFFAIHHRKLDGEFVYYL
                     DIDHLLWGATCHILVKFLREFADFRRMPKIVEPNLTHPPFLDISKL"
     gene            complement(2773..3597)
                     /locus_tag="PK683_01645"
     CDS             complement(2773..3597)
                     /locus_tag="PK683_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07176.1"
                     /translation="MKITFIFILFIYNCSTFTAKKDPFVKDSEDHYRVCFWNVKNLSE
                     KGLKKSGKGQMIIEFAKNCDVISFMEVRSANVDMAAAFEKGFQEHGADYICMEGDPKG
                     KEDSTRTEKYLACVRKEFADDIEKHEFEDKNNDFARAPTFFLFSGIKKILVVPFHSTP
                     GDRNELIQFQKVADFATRNFSDRKIFLGGDFNTGKRYQKPDFLASLTYFVYMNQLIRE
                     ATTFGDQKHDLIFTDAVLAKQCKGKVWRLDEFFPEIDGRKELEKISDHFPVSAECK"
     gene            complement(3927..4379)
                     /locus_tag="PK683_01650"
     CDS             complement(3927..4379)
                     /locus_tag="PK683_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07177.1"
                     /translation="MYENQERGKTFFREGDLDKAVYHFTLALEEEENEELLFYMGLIS
                     NQKKEAVKALNYFYKAIQKNPEYGSPCNEIGVILLRHGREKEAVFWLKKSVRCRMNDA
                     RHIPLYNLAALYKTWNRPERSLQYLYKALDIEPDFDEAKKLRDEIISN"
BASE COUNT         1373 a          995 c          895 g         1313 t
ORIGIN      
        1 cactacgtcg gcatctacag gctttacttt agcaaggagt gcccccagat gttttctaac
       61 cttatagacc gactgtccga aaatatttga atccggtctg gaaaaataga tatattcgaa
      121 aatgcagagg ctctttttcc ttttaccgaa agggaaatag gatttctcac cgaactgatc
      181 aatgactacc atttctccag gttcgatgtc cctcacatat tccgtgtccg tgatatcaaa
      241 tgcgcaggtt tccgatgcaa atacaattgc accgtccggc cgctttccca tcacaagtgg
      301 gcggaatccg ttcgggtctc gaactgcaat cagtttcttt tcagtcagaa gaagaagcga
      361 ataggccccg tccacctgcc gaagagcatt gctgaaagcc gtcagaagat ccttctcgcc
      421 cgatttggcc atcaggtgga cgatgatctc tgtatctatc gtagtctgaa atatacttcc
      481 ttctgcttcc atttttcttc tgatttcata ggaattgacc agattcccat tatgagcaag
      541 agcaagaggc cccaggttgc tttccacccg tagaggctgt gcatttcgca gaaaactggc
      601 ccctgtagtg gaatatctgt tgtgacccac tgctgcagaa ccgcagagtt ccttcagcgt
      661 cgtctgattg aatacagaag caaccagtcc cattcctgca tacctgtaga tatgctcccc
      721 gtctgttgac acaattccgg aagattcttg tcctcgatgc tgcatcgagt agagacctag
      781 gtaagtgaaa ttagaagctt ctttggcacc atagatccca aaaatagcac attcatcctt
      841 cggtttatct tcactggcta taataaaagg ctggaaagat tcagagggtt ctaaataatt
      901 agagttatcg cccatactaa ggacaaattt aatctcttag aaaaacatgc aaacgaaatc
      961 taattacaca ctgattacca ataaaagagg actggaactt gctgcagtca gtttcaataa
     1021 ttccagacaa atcgcaatcg atacagaatc ttcaggatat tatacgtacg tgccgagagt
     1081 ctgtctgatt cagatcacct gtacaggcaa aaactatatc atagatcctc tttcaggcat
     1141 agatttgaga atcctgggcg atatttttga gaatactaaa gtacttaaga tatttcattc
     1201 agcaatagac gatattaagg cattgaagag agatttcagt ttcaaattca aaaacatcgc
     1261 agatacgatg tattcgtcaa aacttttagc attagagcat aattcgctga attttctcgc
     1321 tgaatactat ttgaagatta aactctccaa gacagaacag aaatccaact gggaaaaaag
     1381 gccattggac agaagccagc tggaatatgc ctcgctggat acagcatatc tggaaaccat
     1441 ctgggcaaag atgaacactg aactggaagc acagggactt ctggaagaag cagaatctga
     1501 attcagcaaa ttctgtgaag aagtgtacaa gccgagggaa acagaagaaa tcgcctggca
     1561 taaatttccc aatgtaatga agtttactcc tgagcagaga agaactgtac atgacatact
     1621 tcagtacaga gaagaaaagg caaaacgtct caacaaggca tccttccgga tcgtcaataa
     1681 tgaaattata aagaaaatcg caacagaaaa acatacggaa gaatcccttc ttgcacttct
     1741 cggaaaaaaa gaaggaaagg atgtttttga tcttcttcag aatccaagcg gccctcctct
     1801 tgagaaaaag gacattccca agctggatga aaatatgact cctgcagagg aagtcaaact
     1861 cagacacttt cataagtgga gagacaagat aatgaaaaaa aggaacatcg atcactctat
     1921 gctcctttcc aacagacagt tcctgcagat cctgagaacc aaaccggcag acctggaagc
     1981 tcttgagaaa ctggaaatcc tttccggctg gaaggtaaaa aactacggcc cttctttcct
     2041 gaaaatgctg aaaggagaac cgtatgatga tctcatcact catctgaagc cgataggagc
     2101 aaaggttgaa attcgagaat caaaaactga aattattcag accgactccg ctcagccata
     2161 gtattcataa ttcaggagca agggactctg ctgtaatcat tccgattttc gaaaaagaag
     2221 gaagagatct tattcttctg accagaagaa gtcctcatct gaaaagtcat ccgggacaga
     2281 tctcctttcc tggcggggtt gttgaaaagg gagaatcctt tcaggatacg gcaagaagag
     2341 aatgggaaga agagcttggt tccagttcgg acacgctgga aatcatttct gaatttcagc
     2401 cgatgcatac atttacagga ttcattattc atccgatcat tgccagatat cgaggtgaca
     2461 gcagtttcag tccgaacgaa aacgaagtct cagagatact cgaatttgat cctgaagatt
     2521 tttattcaaa aagatttttt gcaatccatc acagaaagct ggatggtgaa tttgtatatt
     2581 atctggatat tgatcatctg ctctgggggg ctacctgtca cattctggta aaatttctga
     2641 gagagttcgc cgactttcga agaatgccta aaatagtgga gccgaatctg acccatcctc
     2701 cctttctgga catatctaaa ttatgagcag ctgctcatac tgacggcagc gcgtcggaga
     2761 tgtaattatg attcatttac actctgccga taccgggaaa tgatctgaga ttttttcgag
     2821 ttcctttctt ccgtcaattt ccggaaagaa ctcatcgagt ctccagacct ttcccttgca
     2881 ctgcttcgca agaactgcat ccgtaaatat caggtcatgt ttctgatctc cgaatgtggt
     2941 agcctcgcgg atcagctgat tcatatacac aaaataggta agtgaggcaa gaaaatcagg
     3001 cttctgatat cttttgcctg tattaaaatc cccgccgaga aatatctttc tgtccgagaa
     3061 atttcttgta gcaaagtctg ccaccttctg aaactgaatg agctcatttc tgtctccggg
     3121 cgtggaatga aaaggaacga ccagtatttt ctttatccct gaaaaaagaa aaaatgtagg
     3181 tgctcttgca aaatcattat ttttatcttc aaattcatgc ttttctatat catccgcgaa
     3241 ttcttttctc acgcaggcaa gatacttttc tgtccttgtg ctgtcctctt ttcccttcgg
     3301 atcgccttcc atgcagatat aatcggcccc gtgctcctga aatccttttt cgaatgcagc
     3361 ggccatatcg acgtttgctg aacggacctc cataaaactg atcacgtcgc agttctttgc
     3421 aaactcaata atcatctgcc cctttccgct ctttttcaga cccttctccg aaagattctt
     3481 tacattccag aagcagactc tgtaatgatc ctctgaatcc ttgacaaaag gatccttttt
     3541 ggcagtgaaa gtactgcagt tgtaaatgaa gagtatgaat ataaatgtga ttttcataat
     3601 tacggatcct tataattctc tgattttcac ctgcaaaatg cagcagggac tgaagcttac
     3661 tgtggctgcc agtgctttaa aatgtcagtc tgtgcattat tccagggaaa aacctgcttc
     3721 tataaacttt cagtgatttt ataatcattc tttttagtca atgttaattg aatcttcatt
     3781 tcgctgaaga aaaaacggtt tttcaaagcg cagtaattgt tgagtgcagt tatttctttt
     3841 tttcacaaaa tcgcaaaccg aatttcaaaa tgcctgcgtc tgctgtactg tttctgactt
     3901 aatcaatgtt ctaaagaata ggcagatcag ttggaaataa tttcatctct cagtttcttg
     3961 gcttcatcaa aatccggctc tatgtccaaa gctttgtaca gatactgaag tgatctttca
     4021 ggacggttcc aggttttgta aagtgctgcc agattgtaga gtggaatatg ccttgcatca
     4081 ttcatcctgc accggacaga ttttttcagc cagaagactg cttccttttc tctgccatgt
     4141 ctgagcagaa tgactccaat ctcattgcag ggactgccgt actcaggatt tttctgaata
     4201 gctttgtaga aataatttaa tgccttgact gcttcttttt tctgattgga aatcagaccc
     4261 atataaaaca gcagttcttc attttcctcc tcttccaatg caagagtaaa atggtacact
     4321 gctttatcaa ggtcaccttc ccggaaaaag gtctttcctc tttcctggtt ttcataaatg
     4381 aatgtttttg aaaatctgga caaatcctgc tccggctaca gtacaatttt cggatcagca
     4441 aaatactaaa cttaaagaga gtactcaatt tcgggaaaaa acttttatgc gaaatctaat
     4501 gatacataat ataattatgt gtcaaatctc cagatcagat ttcttgccgt aacagcctag
     4561 ggacaattgc aatatt
//
LOCUS       DAOQGJ010000062         4541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10122_length_4541_cov_4.060633, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000062 DAOQGJ010000000
VERSION     DAOQGJ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10122_length_4541_cov_4.060633"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK683_01655"
     CDS             <1..487
                     /locus_tag="PK683_01655"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07178.1"
                     /translation="KTIGDSYMCAGGIPKPSATHAIDAVLAALEIQSFMNQMKSLKEM
                     MGFPYWELRLGIHTGPLVAGVIGEKKFAYDVWGDTVNTASRMESSGYPGRINISGTTY
                     EYVKDFFDCEYRGRINAKNKGEVDMYFVLNLKKELRVSDDSPVSAGNEKFWKKYLALS
                     L"
     gene            614..997
                     /locus_tag="PK683_01660"
     CDS             614..997
                     /locus_tag="PK683_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07179.1"
                     /translation="MESKELSLSSDQKFLIKRLSGRMYGAGIVLTVIGIMLFAEGFSK
                     ELSYLFAGSVNIIIGIFTIFAAKSFQKILDQQGDVLVALITSFKYQSQLYSLQFWIYS
                     AAAFVMLLGTAASFILETSHIKINR"
     gene            complement(1054..2121)
                     /locus_tag="PK683_01665"
     CDS             complement(1054..2121)
                     /locus_tag="PK683_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH07180.1"
                     /translation="MKSRLIKILIIALVPGAILFSKGKRITMPDSDGDTYFDKLERAA
                     GTDPSKNECKPKKCPDASINTIDEAEHFIMILDQSGSMSADFGGNETRMEAAKRVVKD
                     FIQNAPNDLKIGLYTYGKNECSAFDEMQNPFEKMNRTKLIEEVGTIQPSGSTPIGASI
                     SKLREFLSDKRGSFNVLLVTDGGESCNGNPAEEARKLIELNKNSGVAIRLYIAGIGMS
                     DEDGKELKQVAVASQGHYIDIKNSDEMEKIFLTPIQGIVKSLKGMVCLQAEADELIRC
                     ESARLNKIKLVYPRKIDMDIPNEFSPDEKEFLKEQLPKLEEKAQDRIEKFQNVKAKRH
                     EDYQEKIIKYSELIGPHKKKK"
     gene            complement(2296..4401)
                     /locus_tag="PK683_01670"
     CDS             complement(2296..4401)
                     /locus_tag="PK683_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07181.1"
                     /translation="MKGIKFSVRVSLVTLSAVVILLSGGFISLISFYGSRMSIHILVE
                     GLMENICVQTIDKTLSYLKTAHISGEITEFMFNEGLLDMEKKEVLSRYFKSIVEANPQ
                     IVAVFYGNEKDGKFLMQKQMRDDSISEKYIYPSGSKIQTDWKHSNPKFAKDLKSFLED
                     PPGAFEPRTRPWYSDAKNKKDHIWTDIYIFDLDKLPGLTYSTPIYSDKELKGVIGIDI
                     SIKGLSYFLGRQKIGKTGKAFLINKRFEIIAHPLLENEEFHGIDENGHDEEDHEIINS
                     IHVFRDRMKGLSWDEVKKSPAFYVHTHEGETHMNMYYPFPAGPHDWMIGIVVPEDEYI
                     YIIKKNNKIIWGSSFVLIIAAVFVGIIFARRISRPLSQLSREMDEVKTFRITEGEKIN
                     SSLLEVDNMARSFEGMKQGLKSFQKYVPADLVRELISIGKEAVPGGDRRELTVYFSDI
                     ANFTSISESLSPEELVSLLSEYLESSSHKITDFKGTIDKYIGDSIMAFWGAPIPLETH
                     ALNACLAALNVKKGLKMLEAQWKAQGKPPIFKDRIGINTGEVIVGNIGSNSRINYTVI
                     GDTVNLASRIEGLNKYYGTEILISEATYEKVKNDLFTRKVDLVSVKGKTKGTSIYELI
                     SEKKDADADSVKWVSAYEEGLSFYLEKKWQKAVSSFEKARSMNPSDINSESFIKRCSL
                     YIQTPPPEDWDGVFRMTSK"
BASE COUNT         1225 a          943 c          870 g         1503 t
ORIGIN      
        1 caagactata ggcgacagtt atatgtgtgc cggaggcatt cccaagccga gtgcaactca
       61 tgcaattgat gcagtgctgg cggcactgga aattcagtca tttatgaatc agatgaagtc
      121 actcaaagaa atgatgggtt ttccatactg ggaacttcgt ctcggcattc acactggccc
      181 tctggtagca ggtgtcatag gtgaaaagaa atttgcctac gatgtctggg gggatacagt
      241 gaatacggca agcagaatgg agtcgagcgg atatccgggc agaataaaca tcagcggaac
      301 aacttatgaa tatgtgaagg atttttttga ctgtgaatat cgcggcagaa tcaatgcaaa
      361 aaacaagggc gaagtggata tgtattttgt gctgaatctg aagaaggaac ttcgcgtttc
      421 tgatgacagc cctgtgtcag ccggtaatga aaaattctgg aaaaaatatc tcgcactttc
      481 attatgaaaa aactcaacag aaatctgtta atattcgaaa tctgcagaat ttgaaaatat
      541 aaaatatgca ttcatatttg attttatact tggatataca agttttttaa aaaaatgtta
      601 aagtgagggc aaaatggaaa gcaaagaact cagtctttct tcggatcaga aatttttaat
      661 aaaacgatta tccgggcgaa tgtatggagc aggcatcgtt ctgacggtaa tcggaattat
      721 gctctttgca gaaggtttct ctaaggagtt gtcgtattta tttgcaggtt cagtaaatat
      781 aataataggt atttttacta tatttgcagc taaatcattt caaaaaatcc ttgatcagca
      841 aggggatgtt ttggtcgcat tgatcacttc gtttaaatac caatctcagc tgtattctct
      901 gcagttctgg atttattcag cagccgcatt tgtgatgctt ctgggtacgg cagcttcatt
      961 catccttgaa acatctcaca taaaaatcaa caggtaatat gaaagaaaat tacgtctcct
     1021 gttgattctc acgtcctcgg ccaagttctt attttatttt ttcttcttgt gaggaccgat
     1081 cagttcactg tatttaatga ttttttcctg atagtcttca tgtctttttg ctttgacatt
     1141 ctgaaatttt tcaatcctgt cctgagcctt ttcttccagt ttagggagct gctctttcag
     1201 gaattctttt tcatcgggac tgaattcatt cgggatgtcc atatctattt ttctaggata
     1261 gacaagcttg attttattga gtctggcgct ttcacatctg atcagttcgt cggcttctgc
     1321 ctgaagacat accattcctt tcagactctt tacgattccc tgtatgggag tcaggaatat
     1381 tttttccatt tcatcactgt ttttaatgtc aatataatga ccctgtgatg caacagcaac
     1441 ctgcttcagc tcttttccgt cctcatcact cattcctatg cctgcaatat aaagccgaat
     1501 tgcgacccca gaatttttat tcagttcaat gagtttcctt gcttcttctg caggatttcc
     1561 attgcaggac tctcccccgt ccgttactag gagaacattg aaacttcctc ttttatctga
     1621 aagaaattct ctgagtttgg aaatggatgc acctattgga gtgcttcctg aaggctgaat
     1681 ggtgcctact tcctcaatga gttttgtcct gttcattttc tcaaatggat tctgcatttc
     1741 atcaaatgca gaacattcat ttttcccgta tgtatagaga ccgattttca gatcattcgg
     1801 cgcattttgg ataaagtcct tcactactct ttttgcagct tccatcctgg tttcatttcc
     1861 gccgaaatca gctgacatac ttccgctctg atcgaggatc atgataaaat gttctgcttc
     1921 atcaattgta tttatggaag catcgggaca ttttttaggc ttgcattcat ttttggaagg
     1981 gtcggttcct gctgcccttt ccagtttatc gaaataggtg tcgccgtcag agtcaggcat
     2041 tgtaatgcgt ttgcctttgg agaaaagaat ggctcctgga acaagagcaa taatcaggat
     2101 tttaatcagt ctggatttca ttcacacaat catttatact tgcacagata aatcaagaat
     2161 tttatgaatc gaaaaaacgg tccatttcaa ctcttttcgg gatacaggat gttccgcaga
     2221 cggtaaatag cataaaactg tttcatcaga gctgcagacc cagaaatcag ctgaaccttc
     2281 tccagaaaac actcatcact ttgatgtcat tctgaacact ccgtcccagt cttcgggagg
     2341 aggagtctgt atgtaaagac tgcatctttt gatgaatgat tctgaattga tatccgaagg
     2401 attcattgaa cgggctttct caaaagaact tactgccttc tgccattttt tctccagata
     2461 gaaactcagg ccctcttcgt atgcagaaac ccatttcact gaatcggcat ctgcatcttt
     2521 tttttcagaa atgagttcat aaatggaagt tcctttagtc tttcctttca ccgaaaccag
     2581 atccactttt cttgtaaaga gatcattttt tactttttca taagtggctt cactgatcag
     2641 aatttctgtt ccgtaatatt tgttcagtcc ttcaattctg ctcgcaagat tcactgtgtc
     2701 tcctatgacc gtatagttga ttctggaatt tgaaccgatg tttcccacaa tcacttcccc
     2761 tgtatttatt ccaattctgt ccttgaatat cggaggcttg ccttgggctt tccactgtgc
     2821 ttccagcatt ttcagaccct tctttacatt cagggcagcg agacatgcat tgagtgcatg
     2881 tgtttccaga ggaatcggtg caccccagaa tgccataatg gaatcaccta tgtatttatc
     2941 tatagtacct ttaaaatcag taattttatg actgctggat tccagatact ctgaaaggag
     3001 tgaaacgagt tcctccggtg aaagactttc tgaaatggaa gtgaaatttg caatatcgga
     3061 aaaataaact gtcagctccc gtctgtctcc tccaggaact gcttcttttc cgatggaaat
     3121 cagttccctc acaagatctg caggtacata tttctggaag gatttcagac cctgtttcat
     3181 cccctcgaaa gatcttgcca tattgtccac ttcgagaaga gatgaattta ttttttctcc
     3241 ctcagtgatg cggaaagttt tcacctcgtc catttctctt gaaagctgac tgagaggcct
     3301 ggatattctt cgtgcaaata tgattcctac aaagacagct gctatgatca ggacaaatga
     3361 gcttccccag ataattttat tattcttctt tataatatag atatattcat cttcgggcac
     3421 aacaatcccg atcatccagt catgcggacc tgccggaaag ggatagtaca tattcatatg
     3481 agtttcaccc tcatgggtat ggacgtaaaa tgcaggagat ttttttactt catcccatga
     3541 cagacctttc atcctgtctc tgaatacatg gatggaattt ataatctcat ggtcttcctc
     3601 atcatgaccg ttttcatcga tgccgtgaaa ttcttcattt tccagaaggg gatgagcaat
     3661 gatttcaaat cttttattga tcagaaaagc ctttccagtt tttccgattt tctgtctgcc
     3721 cagaaagtag gacagtcctt ttatgctgat gtcgattccg atgactcctt tcagttcctt
     3781 gtcggaatag atgggagtgc tgtaagtcag accaggcagc ttatccagat caaagatata
     3841 aatatcagtc cagatatgat cctttttatt ttttgcatcg gaataccagg gacgtgttct
     3901 gggttcgaat gcgccgggag gatcttccag aaagctctta agatcttttg caaatttagg
     3961 attggaatgt ttccagtcag tctggatttt gctgcctgaa ggatatatgt acttttcact
     4021 gatgctgtcg tctctcatct gcttctgcat caggaatttt ccgtcttttt catttccgta
     4081 aaaaactgca acgatctgag gatttgcctc cacaatgctt ttgaagtatc tggaaaggac
     4141 ttcttttttt tccatatcca gaagaccctc attgaacata aactctgtga tttcaccgct
     4201 gatatgggct gtcttaaggt aagacagagt cttgtctatt gtctgcacac atatattttc
     4261 catcagtcct tccacaagta tgtgaattga catcctgctt ccgtaaaagg aaatcagact
     4321 gataaatccg cccgaaagaa gaatgacgac tgctgaaaga gtcactagac tcaccctgac
     4381 cgaaaatttg attcccttca taattccatc ctgttgcgat aatctgtttt atataaaata
     4441 atgcatatga atatttttgc cctgcagtct tttcattgaa ttatttcata ttttgccgat
     4501 taatcaacag gaaaaaaatc agtatctgtt ttgtgaagca t
//
LOCUS       DAOQGJ010000063         4482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10331_length_4482_cov_3.376101, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000063 DAOQGJ010000000
VERSION     DAOQGJ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10331_length_4482_cov_3.376101"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1432
                     /gene="purT"
                     /locus_tag="PK683_01675"
     CDS             242..1432
                     /gene="purT"
                     /locus_tag="PK683_01675"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNH07182.1"
                     /translation="MAKENLKIGSPFAANSTKILLLGSGELGKEFVLEAQRFGIHVMA
                     VDRYPNAPAMQVAHESKVIDMLNAQELEKLVRSYNPHYIVPEIEAISTETLVKLESEG
                     FNVIPSGKAVNLTMNREGIRDFAAKELGLKTSAFEFADSLEDFHDKVMKIGIPCVVKP
                     IMSSSGKGQSIIRNESEIEKAWNYGQTGGRAGKGKMIVEEFIPFDFEITLLTVRHAAG
                     TTFMDPIGHLQVNGDYVESWQPQPMSENALKKAKDIAEKVTAGLGGYGIFGVELFVKG
                     DEVYFSEVSPRPHDTGMVTMISCTYSEFALHVRAILGLPIPKLRYHGPAASAAFLAEG
                     NLDGPEFEGFEEAMQDSDTSFRLFGKPEVKGKRRMGVALALADSIEKAREKARAAVSK
                     IKVI"
     gene            1429..2049
                     /locus_tag="PK683_01680"
     CDS             1429..2049
                     /locus_tag="PK683_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07183.1"
                     /translation="MKEEFLLSLQNSSVSAESAESVFRRLRDMYSEPERRYHTLRHIR
                     DIQSGIIEYRNLFSHYDAYIFACWYHDAVYDSKSKTNEEDSAELAVRELSGLGIPEKT
                     VLLCRDAVLSTKRHIPVPETEEMKLFLDLDLKILGANDERYEEYKKEVRSEYSWVPEE
                     TYRKERANVMKYFLKRDRIYFTEMMHQKFDAQARKNLKQEIEELEK"
     gene            2039..3037
                     /gene="rfbB"
                     /locus_tag="PK683_01685"
     CDS             2039..3037
                     /gene="rfbB"
                     /locus_tag="PK683_01685"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNH07184.1"
                     /translation="MKNNRKLQNMLVTGGAGFIGANFIQYAAKKGHRGRIVNLDALTY
                     AADSAHLNGIESKLNYTFIHGNILDRNLLKRIFSEYDIDTVVHFAAESHVDNSIESPD
                     IFIKTNVNGTFELLSAAREYWKDRKNVLFHHISTDEVYGSLGQTGYFTETTPYDPSSP
                     YSASKAGSDHLVMAWHRTYGLEVTMSNCSNNYGPFQNREKLIPLMITNALTGKKLPVY
                     GDGKNVRDWLYVEDHCDAVWTILEKGENGRTYNVGGNNEWENIRIVKHICQVIGELTD
                     KPVSHYEALIEYVKDRPGHDRRYAIDAARIREELGWTPKETFETGIVKTVKYYLSK"
     gene            3113..3691
                     /locus_tag="PK683_01690"
     CDS             3113..3691
                     /locus_tag="PK683_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07185.1"
                     /translation="MDSEAYVHNDKIMKTFCFIAIFFFSACTSSLKVTEIKSGMRLES
                     AVEKDVDFFFQCRMKNRIKKILGYNWEILYKDGRFVYFGYPESMFSSEMKVQNLYKVK
                     MEELQKDFPSFETVEGHHIQRAISEAIHKDVKIESRILIQNIDWKPSLQKGFIHIDAV
                     WKYQADSNPEVTKKYQMQLDMKSLKVISKKEI"
     gene            4112..>4482
                     /locus_tag="PK683_01695"
     CDS             4112..>4482
                     /locus_tag="PK683_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HNH07186.1"
                     /translation="MNFYLLEPRFEIYSLSHNLAIAIIFLGCISLYFFRKKLSETPKA
                     DLLFRGLLTAVLIGGEIILQYWYVSTNQWKKEYSLPLQLCDFSIFASALLLVTGNRKI
                     FEIVYFTGIGGGLIAVLSPEL"
BASE COUNT         1368 a          843 c         1030 g         1241 t
ORIGIN      
        1 cctaatacct tctgaaaatt acagaatcta gatagcatat tgtatttgcg taaggagatt
       61 taggataact caaaatcttg aaattttggt cccgttcttt tactctgtca atcgggttca
      121 ttgtctgaat cagagtatag ctctgtctgg cagctttgtc aatcagtttg cacagcggaa
      181 agaaacaccg aacccctaaa aaagaaattg cattcacagt cttttatttt tggattgaac
      241 tatggctaaa gaaaacttga agatcggctc tccgtttgcc gcaaattcta caaaaatact
      301 tcttcttggt tcaggggaat taggaaagga attcgttctc gaagcacagc gtttcgggat
      361 tcatgtgatg gcagtggaca gatatccgaa tgctcctgca atgcaggtcg ctcacgaatc
      421 caaggtcatc gatatgctca atgctcagga gctggaaaaa cttgtgcgca gttataatcc
      481 tcattatata gtacctgaaa ttgaagcaat ttccactgag actctggtca agctggaatc
      541 ggaaggattc aatgtaattc cttccggaaa ggcagtcaat ctgacaatga acagagaagg
      601 catccgtgat tttgcagcca aggagcttgg gctgaagaca tccgcttttg aatttgcgga
      661 cagcctggaa gattttcatg ataaagtaat gaaaattggc ataccatgtg tagtcaagcc
      721 gatcatgagt tcctcgggaa agggacagag cattatccgc aatgaatctg aaatcgaaaa
      781 agcctggaac tacggtcaga caggaggaag ggcaggaaaa ggaaagatga ttgtggaaga
      841 gtttattcct tttgattttg aaatcactct tcttactgtg cgtcacgcag ccgggacaac
      901 gtttatggat ccgatcggac atcttcaggt gaatggcgat tatgtggaat cctggcagcc
      961 ccagccgatg agcgagaatg ctctgaaaaa ggcaaaggac attgcggaaa aagtgactgc
     1021 cggtctcgga ggatatggca tatttggcgt tgagcttttc gtcaaagggg acgaagtcta
     1081 tttcagtgag gtgtctcccc gccctcatga cacagggatg gtgacaatga tctcctgcac
     1141 ttattctgaa ttcgctcttc acgtaagagc aattctggga ctgccgattc ctaaactgcg
     1201 ttatcacggt cctgctgcat ctgcggcatt cctggcagaa gggaatctcg acggtcccga
     1261 gtttgaaggc ttcgaagaag caatgcagga ttcagacact tcattccggc tctttggaaa
     1321 accggaagtc aagggaaaaa gaagaatggg cgttgctctt gcactggcag acagcataga
     1381 aaaagccaga gagaaggccc gggcagctgt atctaaaata aaggttatat gaaggaagag
     1441 tttctcttat ctctgcagaa ttcctccgtt tcggcagagt ctgcagagtc tgttttcaga
     1501 agactgcgtg atatgtattc tgaacctgag agaaggtacc atactctcag acatatcaga
     1561 gacattcagt ccggaatcat tgaatacagg aatctgttca gtcattatga tgcatatata
     1621 tttgcatgtt ggtatcatga tgcggtctat gattcaaaat cgaagacaaa cgaagaagac
     1681 agtgctgagc tggctgtgag agaactttcc ggtctcggta tccctgagaa aacagttctg
     1741 ctctgcaggg atgcggttct ctccaccaaa aggcatattc ccgtacccga gactgaggaa
     1801 atgaagctgt ttctggatct ggatctgaaa attctgggtg cgaatgacga aagatacgag
     1861 gaatataaga aagaagtccg ttctgaatac tcctgggttc ctgaggagac ttacagaaaa
     1921 gaaagagcaa atgtaatgaa atattttcta aaaagggaca ggatatattt cacagaaatg
     1981 atgcatcaga aatttgatgc tcaggcaaga aaaaatctga aacaggaaat tgaagaactt
     2041 gaaaaataac agaaaactgc agaatatgct cgtcacgggt ggagccggtt ttatcggagc
     2101 caacttcatt cagtatgctg caaagaaagg acatcggggc agaatcgtca atctggatgc
     2161 actgacttat gctgctgatt ctgctcatct gaatggaatt gagtctaagc tgaattatac
     2221 attcattcac gggaacatac tcgacaggaa tcttctgaaa aggatatttt ctgaatatga
     2281 catcgatact gttgttcatt ttgcagctga gagtcatgta gacaattcca ttgaaagtcc
     2341 tgatattttc attaagacaa atgtcaacgg tacattcgag cttctttctg ctgcgagaga
     2401 atactggaag gacagaaaga atgttctgtt tcatcatatc tcaactgatg aggtttacgg
     2461 ttcactcggg cagacaggat attttacaga gacaacaccc tatgatccct cctctcccta
     2521 ttcagccagc aaggccggtt cggatcatct tgtgatggcc tggcacagaa cctacggact
     2581 ggaagtgacg atgtccaact gttccaataa ctacggaccc tttcagaaca gagaaaaact
     2641 gattccgctg atgatcacaa atgcactgac aggaaaaaaa cttccagtat acggtgatgg
     2701 aaaaaatgtg agagactggc tttatgtaga agatcactgt gatgctgtct ggacaatcct
     2761 cgaaaaaggc gagaacggaa ggacatacaa tgtaggcggg aacaatgaat gggaaaatat
     2821 ccgcatcgta aaacatatct gccaggtgat cggggaactg acggataagc cagtttcgca
     2881 ttatgaggca ctgattgaat atgtaaagga cagaccggga catgacagac gttatgcaat
     2941 tgatgcagcc aggatccgtg aagaactcgg ttggactccg aaggaaacat tcgaaaccgg
     3001 cattgtaaaa acggtaaaat attatctttc aaaatgaaga atgttttttg caaacggttc
     3061 atttttccag agacgatttc ttctgtagac gggtctgaga cccgtctcta cgatggattc
     3121 ggaggcatat gtgcataatg ataaaataat gaaaactttc tgttttattg cgattttctt
     3181 tttttccgcc tgcacatctt ccctgaaggt gactgaaatc aaatccggga tgaggctgga
     3241 atctgcagtg gaaaaggatg ttgatttttt ctttcaatgc aggatgaaaa acaggatcaa
     3301 gaagattctg ggatacaact gggagattct ttacaaggac ggacgttttg tgtatttcgg
     3361 ttatccggaa agtatgtttt ccagtgaaat gaaagtgcag aatctgtata aggtgaaaat
     3421 ggaggaactt cagaaagatt ttccttcttt tgaaacagtc gaaggacatc atattcagag
     3481 ggccatttca gaagcaattc ataaagatgt gaaaatagag agcagaatac tgatccagaa
     3541 tattgactgg aagccttccc tgcagaaggg ttttatccat atagatgctg tatggaaata
     3601 tcaggcagac tcaaatcctg aagtcacgaa gaaatatcag atgcaattgg atatgaagtc
     3661 attgaaagta atttcgaaga aggagatctg agactgaata aaccggcata tgcagatgtt
     3721 atatgctccc ttgacactgt aattttgata aattatcata aaaatccagc agcttaacgg
     3781 tttgcaatga atcagtaaaa aacctctttt tcttacttcc tgtatattta ggcggctttc
     3841 tgactggcat attcataatg cttgatgcag gagacgtcaa attctagttt tcaagagtct
     3901 ttcgtatttc atttttcaag acttagcaaa aaagcttcca ttcggaaagg tagaatgttt
     3961 tacgaaaatt gcatagaaga ttcaaggttt acgaaacacc ggaaggctcc cggccagaaa
     4021 cagtgcaatt atttatgaga gttagtataa tataaattaa agatattata taatatattg
     4081 acttactata atgcaataaa aaagatggct tgtgaatttt tatctcctgg aacctcgttt
     4141 tgaaatttat tccctttccc acaatctcgc aattgctatt atttttcttg gatgcatctc
     4201 tctttatttc ttcagaaaaa aactttctga aactccgaag gctgatcttt tgttccgagg
     4261 tctgctcact gctgttctga tcgggggcga gatcattctt cagtactggt atgtttccac
     4321 aaatcagtgg aaaaaggaat acagtcttcc tctgcagctc tgtgatttca gcatatttgc
     4381 cagtgcgctg cttcttgtga ctggaaaccg aaaaattttt gaaattgtct attttaccgg
     4441 gattggtggc ggtctgattg cagttctgtc tccggaactt tg
//
LOCUS       DAOQGJ010000064         4455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10431_length_4455_cov_3.162955, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000064 DAOQGJ010000000
VERSION     DAOQGJ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10431_length_4455_cov_3.162955"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..414
                     /locus_tag="PK683_01700"
     CDS             58..414
                     /locus_tag="PK683_01700"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNH07187.1"
                     /translation="MLINVCKSKIHRATVTEANLNYIGSLTVDVELLEAAQMLPYQKV
                     SVVNVNNGARLETYLIEGERGSGTICMNGAAARLGHTGDIIIIIAYGLIDEKEYPKNF
                     SPIVVHVDEKNRLKKI"
     gene            566..1519
                     /locus_tag="PK683_01705"
     CDS             566..1519
                     /locus_tag="PK683_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07188.1"
                     /translation="MNSTFRITISLLILFFALELASEAAPGSTTPAPTSAASNTSAPA
                     KAVDPEKAGEARKARRKDKAAELKEYQTIPIADPNLEIKGISYFRKVAASGRGEMLDV
                     QFSLYNKDVLEGKDKKDKEYAVFVLGLHETTAPVHYPSKWRKTDPKKNVKLIRFHTLV
                     GAEGDQLKDAKFISDAALNQMASDKNAALLDERSVAFHESEPTFEDYLYYLTRNPEKA
                     LKVKVFGYNNPERDKYVVTNLKVDNAEIARDMNKEAEKHTYTIQYNKYLTTVTTHHFS
                     LYRPDFLFYNKVVILVFDPDRPKNKLIFRSIQDIGKIRLKG"
     gene            complement(1945..2184)
                     /locus_tag="PK683_01710"
     CDS             complement(1945..2184)
                     /locus_tag="PK683_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07189.1"
                     /translation="MHRILFCPYEDAVEVPDRPVQIRQNDEEGRFKMSPTMIIIVAVG
                     IACWAYVIFSVGLRLIANSTSQKLKKTIEEALKNK"
     gene            complement(2225..3346)
                     /locus_tag="PK683_01715"
     CDS             complement(2225..3346)
                     /locus_tag="PK683_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNH07190.1"
                     /translation="MDKLKTKLGLFRLSLIDRYIFSEFVRVFLGTVVLLTGILLISQI
                     TDNLKGFLSTKQPQYHIGLYILFSLPKMMIVVIPPSLMFSVCFVIGQFNSNKELVSIM
                     AAGVSFYKAVRTIFVFGSVLTVFVLFFSEMVVRPANSLAAFEFSMIEKGIGTKKDLVY
                     QLHIKGSQGFYYLYWYDHEKQKVSGGFNYLKLKEDAKPEFVISAQSAQYLKDEKVWKL
                     DKVEEIGFTENLTVKSYKKSHEKKEAFPEDPSYFAKPMKSVEEMNFLELGREIVMRQS
                     KGMPYGELEVERNVILANPLMCLLVVFIGAVIGAMTPKGGAIVSLTYTILFVLVYYVF
                     FSVGKSLGENGGLHPVIAVWLTPSFFLSMCFYLYKKFNM"
     gene            complement(3435..3605)
                     /locus_tag="PK683_01720"
     CDS             complement(3435..3605)
                     /locus_tag="PK683_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07191.1"
                     /translation="MVVAEEKTFNTSDLYENMIQQKIENMDTVEKIQLKGILSRLIDS
                     IFEKKEAQKNNK"
     gene            3818..>4455
                     /locus_tag="PK683_01725"
     CDS             3818..>4455
                     /locus_tag="PK683_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07192.1"
                     /translation="MIGLVLFPSLIFSLIFPLLKNRIFSNFVKFLPLAVIAYCIAHLT
                     GDYFYFQNANKHLGYEGFVFFGKDFTVLLVSVFKNQLAEMAVSAVFLISFLLIGIKIL
                     GRFDFDRKEDSFRKKLIRRAVWASVLIVLIRGGIQPSLLSPGNAIVTNNPLLNQFVLN
                     GIFTTVQDFRTEKFPSIQTMKLTESIPLVRSLISYNGAEFIDGPYPILRKTVP"
BASE COUNT         1386 a          950 c          834 g         1285 t
ORIGIN      
        1 ccggcaaaat ttttaaagtt ctattcgagg actgaaaaaa actctgaggc ggcaactatg
       61 ctaatcaacg tctgcaaaag caaaatccac agggctaccg tgacggaagc aaatctgaac
      121 tacattggaa gtctgaccgt agatgtcgaa ctccttgaag cggctcagat gcttccctac
      181 cagaaagtca gcgtggtcaa tgtgaacaat ggggcacgcc tggaaacata tctgattgaa
      241 ggggaaagag gttcaggcac aatctgcatg aacggagcgg cagcaagact cggacatacc
      301 ggcgacatca ttatcatcat tgcctatggt cttatagacg aaaaagaata ccccaaaaac
      361 ttttctccta ttgtagtaca tgtagacgaa aaaaataggt tgaaaaagat ttgaatcctg
      421 aaacattgta agaggaataa agttcctggt aaacttcagg cagtgaaatt ttcaattgaa
      481 cttgaatttt tcccagtttg aggtctaatt tgcacttgag ctttttacga taatacaaat
      541 taagaaaaag atacggacga tctagatgaa cagcactttc agaattacta tttcgctact
      601 catactgttt tttgctttag agctggcctc tgaagccgct ccaggcagta caacacctgc
      661 tccaaccagc gctgcctcaa acacttctgc tcctgctaaa gcagtagatc cagaaaaagc
      721 aggagaagcg aggaaagcca gaagaaaaga caaagctgcg gaactaaaag aatatcagac
      781 aattccaata gcggacccaa accttgagat caaaggcatc agttatttcc gcaaagtggc
      841 ggcaagcggt cgcggggaaa tgctggatgt acagttctca ctttacaata aggatgtttt
      901 agaaggaaaa gacaaaaaag acaaagaata tgcagttttt gtacttggtc ttcatgaaac
      961 aactgctccg gttcattacc catccaagtg gagaaaaact gatcctaaaa aaaatgtgaa
     1021 actgatcaga tttcacactt tagtaggtgc tgaaggcgac cagctgaaag atgcgaaatt
     1081 tatcagtgat gcagctctga accagatggc ttccgataaa aatgcagcat tgctggatga
     1141 aagaagtgta gctttccacg aatctgagcc tacttttgaa gattatctct attatctcac
     1201 aagaaaccca gagaaagctt taaaagtaaa agtattcggt tacaacaatc ctgaaagaga
     1261 caagtatgtt gtcacaaacc tcaaagtaga taatgcagag attgccagag atatgaataa
     1321 ggaagctgaa aagcatactt atactatcca gtacaacaag tatctgacta ctgttacaac
     1381 tcaccatttc tctctttaca gacctgattt cctgttctac aacaaagtag tcattttagt
     1441 atttgatcca gacaggccaa agaataaact gatctttcgt tccatccagg acatcggaaa
     1501 aatcagactt aaaggctgat cttccaattt ttagcccgcc gacaagcggg ctttttgttt
     1561 ctgctcaaaa aactcactga ctttcaaaga gaaaccgacc aaaacctcac tgaataattc
     1621 tgcatcaatc tcttttttcc attccgttct tcgatttttc cagacttgga tggaaattga
     1681 aactggatca ataatcccct tgttctcatt caaaacaaca gtaatgtcgg gcctgacagt
     1741 atctaattca tatcgatcaa aacttatctt cgatttttaa aaaactctcg aactgagagc
     1801 agaaatcctg caactgaaat tactgtcaaa atcaaatcca cttagggctt aaataactcg
     1861 tatgttgtag ctaaggctac cagaagaccg atagcaataa atcctaaaag cagcagtaag
     1921 ttatctaaaa attccaaagc atccctactt attcttcaaa gcttcttcga ttgttttttt
     1981 taatttctgt gatgtactgt tcgctattaa ccgaagtccg acagaaaaaa ttacataagc
     2041 ccagcaggct atgcctacag ctacaataat gatcattgtt ggactcattt tgaatctccc
     2101 ttcttcatca ttctgtctaa tttgcactgg cctgtcagga acttcaacag catcttcata
     2161 cgggcagaac aggattctgt gcacttgttc tgtcttattc cagcagttca ggtatgaata
     2221 aaatttacat attgaatttc ttatacagat aaaaacacat ggagaggaag aacgatggag
     2281 tgagccagac tgcgattaca ggatgaagcc ctccgttttc acccagagat tttccgacag
     2341 agaaaaatac atagtagaca agcacaaaca gaattgtata agtcagagag acaattgccc
     2401 cgcccttggg agtcattgca ccgatcactg caccaatgaa aacaaccaga agacacatca
     2461 gaggatttgc cagaatcaca tttctttcca cttccagttc accgtaaggc attcccttgg
     2521 actgtctcat tacaatctct ctgccaagct caaggaagtt catttcctcc accgatttca
     2581 tcggttttgc aaaataggaa gggtcttcag gaaatgcttc ctttttttca tgcgattttt
     2641 tgtaggactt gactgtcaga ttttcagtaa aaccgatctc ctccaccttg tccaatttcc
     2701 agaccttttc gtccttcagg tactgagcgc tctgggcaga aattacaaat tcaggcttgg
     2761 catcctcttt cagcttcaga tagttgaatc ctccggatac cttctgcttt tcatgatcat
     2821 accaatacag ataataaaaa ccttgacttc ccttgatatg gagctgatag actaagtcct
     2881 ttttggttcc gattcccttc tctatcatgg aaaattcaaa tgctgccaga ctgttcgcag
     2941 gtcgaacaac catttcactg aaaaacagaa caaatactgt cagaactgaa ccaaatacaa
     3001 aaatggtccg aaccgccttg tagaaggaaa ccccggccgc cattattgaa accagttcct
     3061 tattgctgtt gaactggccg ataacaaaac agacagagaa catcaatgag ggagggatga
     3121 ctacaatcat catttttggg agagagaaca gaatatagag accgatatgg tactgaggct
     3181 gtttggtgga cagaaatccc ttcagattgt cagtaatctg tgaaatcaga aggattccag
     3241 tcaagagcac gacagtaccc agaaatactc tgacaaattc agagaatatg tatctgtcta
     3301 tcagagaaag cctgaaaagg cccagcttcg tcttaagttt atccaacaga accagactga
     3361 gattttttgc gggaaagtaa agtcaatatt tgaggaagga gtcaaccggg ggaatctccc
     3421 ccgggatata atttttattt gttgttcttt tgtgcttcct tcttctcgaa tatagaatct
     3481 atcagtctgc tcagtattcc tttcagctga attttctcta ctgtgtccat attttcaatc
     3541 ttctgctgga tcatattttc ataaagatcc gatgtattga aagttttttc ttccgcaacg
     3601 accatattcg tcactcctgt aaaaaaagtt gccagccttc ctcgtcgaaa gagcatagct
     3661 catggaaaaa atcttcctac tatatctgac ggcaggtttt tggatttttc tcatttttag
     3721 actgattttt ttctttcatt attgggacca aattaaaaat tcttcctctg ccgaaatctt
     3781 ttctgttcta ttaaatggca cgagatttga tttttccgtg attggactcg ttctttttcc
     3841 gagtctcatt ttcagtctga tatttcctct tctcaaaaac aggatttttt ccaatttcgt
     3901 gaaatttctt cctttggcag tcattgccta ctgcattgct caccttacag gtgattattt
     3961 ctattttcaa aatgcaaata agcatttagg atatgaaggc tttgtattct tcggaaaaga
     4021 ctttacagtg ctgctcgttt ctgtatttaa aaatcagctt gctgaaatgg cagtatcggc
     4081 tgtttttctg atttcctttc tgctgatcgg aatcaaaatt ttaggaagat ttgatttcga
     4141 caggaaagaa gattcattcc ggaaaaaatt gattagaaga gcagtctggg cttccgttct
     4201 gattgtactg attcgaggag gaattcagcc gagtctctta agtccgggaa acgcaattgt
     4261 cacaaacaat ccccttctga atcagtttgt gctgaacggc atattcacta cagttcagga
     4321 tttcagaaca gaaaaatttc catccattca gacaatgaaa ctgactgaat ccattccgct
     4381 tgtcagatcg ctgatctcct acaatggagc tgagtttatt gacggcccct accctattct
     4441 aagaaaaact gttcc
//
LOCUS       DAOQGJ010000065         4445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10469_length_4445_cov_3.209112, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000065 DAOQGJ010000000
VERSION     DAOQGJ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10469_length_4445_cov_3.209112"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PK683_01730"
     CDS             complement(<1..1346)
                     /locus_tag="PK683_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNH07193.1"
                     /translation="MRERIRSFLKISGQKSVYLYSVVVGMLSGLMAGIFMLLLEKSSE
                     YIQYITSDLFLSHRISSDGMNIEWSSKLLLIIFLPALGGLLTGLLVHFLCREASGMGT
                     DSMIYAFHHKEGQMSTRIPFIKSIATVITLSTGGSGGKEGPISQIGAGFGSMVARLAQ
                     AGARARRTLLLAGTAGGLGAVFHAPLGGALTAAEMVYKEDVESDALLPCVISSSTAYL
                     INQTFFPKGTVFSLHSDISYSFKEFPFYFILGVLCFMGGKFFIQAFQIVQKWNETLKI
                     PPYVKPAIGGLAVGVLAVFFPEAAGTGEKFIQKLLHGEFQEIFRTGHLFSILLFILFF
                     VLKTFSSALTIGTGGSAGVFGPSLYAGCMIGGVMALVSSLVLGTKVSFAGYMLVGMGA
                     FYSGVASAPIAGMIMTCEMVGSYYLLPPLMLVTIISFSLSNKFSIYKNQLLNRFASP"
     gene            2023..2781
                     /locus_tag="PK683_01735"
     CDS             2023..2781
                     /locus_tag="PK683_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNH07194.1"
                     /translation="MRVGLFGFGKTGKAVASVILRNKEIVLEWVIRKTSKLEHRSVPE
                     FLGEESDEPGLIYSADHTDFGCLLDEQPVEAIIDFSSESGLDYYGSEAVRRNIIIISA
                     ISHYSEESVTKLKKFSERIPVFWSPNITLGINYLIVSAKYLKKIAPMIDFAVVEEHFK
                     DKPEISGTAKAIARNLDIDTDEIKSVRAGGIIGKHEIICGFPFQTVRLVHESITREAF
                     GNGAIFVAKSMNGRDKGFYTFEELLLPYFMQSPY"
     gene            3037..3570
                     /locus_tag="PK683_01740"
     CDS             3037..3570
                     /locus_tag="PK683_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07195.1"
                     /translation="MKILMICISVLSCIVGSGLAALVFMNRDIVILRSAGKIFPILLT
                     GIYIYRMIISIVIITAVSPSVFFLAERIRQILLKENQEKDISLPARYFLSALHLFLYG
                     FIFTASRYRGPLSAAVFGYIITYTVFFSVVDYSLHRSLKKQRFLGQLLYSLILLIFVI
                     WALYVANAVFSFGRIVD"
     gene            complement(3750..4136)
                     /locus_tag="PK683_01745"
     CDS             complement(3750..4136)
                     /locus_tag="PK683_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07196.1"
                     /translation="MWEFLQTTPERLRRSSYRPTWKTFAGVPADHPESLRRSSYRPTW
                     KTFVGVPTEHSQKAFGRSYRFTRKAFVGVPTDQPGKASQEFLQIHPESLVGVPTDQLG
                     QPFVGVPTDNSERLRRSSYRSPGKAS"
BASE COUNT         1318 a          902 c          976 g         1249 t
ORIGIN      
        1 ggagaggcga atctgttcag aagctgattc ttgtagatgg aaaatttatt gctgagggaa
       61 aatgatatga tcgttacaag catcagaggt ggaaggagat aataacttcc caccatctcg
      121 catgtcataa tcattcctgc aataggagca ctggctacac cggaataaaa tgcacccatt
      181 cctacaagca tataacctgc aaaagaaacc ttggttccga gtacaagaga ggacacaagg
      241 gccatcactc ctccgatcat gcaccccgca tacaaagaag ggccgaatac gcccgcagaa
      301 ccccccgtgc ctattgtgag agccgaactg aatgtcttga gcacgaaaaa aagaatgaag
      361 agcagaatgg aaaacagatg gcctgttctg aagatctcct ggaattctcc atgaagaagt
      421 ttctgaatga atttttctcc tgttcctgca gcctctggaa agaagacagc gaggacgcct
      481 actgcaagac cgcctattgc aggtttgaca tagggaggaa tcttcagagt ctcattccat
      541 ttctgcacaa tctgaaatgc ctgaatgaaa aatttcccgc ccataaaaca gaggacaccc
      601 agtataaagt agaaagggaa ttccttgaaa gaatatgata tatccgaatg taatgaaaaa
      661 acagttccct tcggaaaaaa tgtctgattg atgagatatg cagtagagga agaaatcacg
      721 cagggaagaa gggcatccga ttccacgtct tccttgtaga ccatctctgc tgcagtgagg
      781 gctcctccga gaggcgcatg aaagactgct ccaaggccgc ctgccgttcc ggcaagaaga
      841 agagtccttc ttgctcttgc tcctgcctgt gcaagcctcg ccaccatcga cccgaatccc
      901 gcaccgatct gtgaaatagg accttccttc cccccgctcc ctccggtaga cagcgtaatt
      961 acagtagcta tggatttaat gaaaggaatt cttgtgctca tctgtccttc cttgtgatga
     1021 aaggcataga tcatcgagtc agttcccata cctgaggctt ccctgcagag aaaatggaca
     1081 agaagccctg tcagaagccc tcccagtgca gggagaaaga taatcagaag aagttttgac
     1141 gaccattcaa tattcattcc atcagaactg attctgtggg ataagaaaag gtcagatgta
     1201 atatattgta tatattcaga agatttttcg agaagaagca taaaaatgcc cgccatcagc
     1261 ccgctgagca tcccgaccac aacagaatac agatagacgg atttttgtcc tgaaattttt
     1321 aaaaaactgc gaattcgttc tctcatcttt ttgcagcaaa aagcattacc tgaataaatg
     1381 aatgctcgtc tttttcagaa atatcctgct gatgaatatg taaacagtag tgaaaataac
     1441 tgtaatattt ttttttattc atagagacaa tgataaaact ggtatatgtc tatgcaaata
     1501 caaaattttc attcgttgac tgccgacatc atatagaagt gtattcggaa tactgatgcg
     1561 attttgtcat attctggatt ttgagcatta ttattcttaa ataattatac tgattaccaa
     1621 aagtaattgc gctttgtaca ttcgaagact ttgattttac aggctcaata aagtagaaat
     1681 acgcaaagac ttttgaatat gagtataatt tcattggcgg aagaatgaaa ttttcaggca
     1741 gctccgtact gccgaaggat aatcactcct taaaacttgt cttgcggggc aattgcaagg
     1801 tccagtccca ggaactgagg aagcagatta gaattcccgg tactgcaata tcaaataatt
     1861 attcattaca tatataagtc agaccagagc tgcaaagttg ttttggatat ataagatcag
     1921 taaaaaaaag cttgtctttt gggcttgatt gctatagatt gactttattc gcatgggatg
     1981 agattttcat ccgactaatc tcaatattaa agaggtaaaa agatgagggt tggactgttt
     2041 ggttttggca aaactggaaa agctgttgca tctgtaatct tgagaaataa ggaaattgta
     2101 cttgagtggg ttattcgtaa aacatccaaa cttgaacatc ggtcggttcc tgaattttta
     2161 ggtgaagaat cagatgaacc agggttaata tattctgcag atcatactga ttttggctgt
     2221 ctcctggatg aacagccagt ggaagcgatc attgattttt catcagaatc aggattggat
     2281 tattatggat cggaggcagt aagaagaaat attataatta taagtgcaat atcgcattat
     2341 tcagaggaat cagtaacaaa gctaaaaaag ttttctgaaa gaattcctgt gttttggtct
     2401 ccaaatataa ctctcggaat taattatctc attgtttcag ctaagtacct caaaaaaata
     2461 gctccaatga ttgattttgc ggtagttgaa gagcatttta aagataagcc ggaaatttct
     2521 ggaacagcaa aagccatagc aagaaattta gatattgata cagatgaaat taaatctgta
     2581 cgggcaggcg gtatcatagg aaaacacgaa attatttgtg gatttccatt tcagactgtc
     2641 cgtttggtcc atgagtctat aacacgagaa gctttcggga atggtgcaat atttgttgcc
     2701 aagagtatga atggcagaga caaaggattt tatactttcg aggaacttct gcttccttat
     2761 tttatgcaga gcccttactg aaaaatggga atgtttaaga attaaattac tctataagga
     2821 aaatggtaag tttgttgaat tgatttccga aaatgtctga attcccaccg gaagtttttt
     2881 cacagttcaa gagtacatga agcagagtag ggtcaagcct ttttgtctcc cgaacctgac
     2941 ctgattcctg agaaaggcaa aacaaaaacc gaaagagtat gccatatttc tgttgcggat
     3001 attccgtgag taaggaaaaa ttgaaaaaac cagactatga aaatacttat gatctgtatt
     3061 tccgtgctca gctgtatcgt cggttccggt ctcgcagctc ttgtttttat gaacagggat
     3121 atcgtgatcc tgcgcagtgc aggaaaaata tttcccattc tcctcacagg catttacata
     3181 tatcgaatga taataagtat agttattata accgcagttt caccgtccgt attctttctg
     3241 gcagaacgaa tcagacaaat cctcctaaag gaaaatcagg aaaaagatat ttctcttccc
     3301 gcaagatatt tcctttcggc cctgcattta tttctgtacg gatttatttt cacagcatcc
     3361 cggtacagag gccctctgag cgccgccgtt ttcggatata tcattacata taccgtattc
     3421 ttttctgtcg tcgattattc cttgcacaga tctctcaaaa agcagagatt tctaggacaa
     3481 ctgctgtatt cactgatcct gctgatcttc gtaatttggg cgttgtatgt cgcaaatgct
     3541 gttttctcct ttgggagaat tgtggattag gcaggctgcc catactgtca agataaggat
     3601 gattcaccac ggatacacac ggattcacac agagaatgaa tccaaatatc tgtgttgatc
     3661 gcgactgtgt aaaggcataa aaaaatgata ctcacgcgta gcttgcactg agcttgttca
     3721 aatcctttcc aggttggtct gtagggctcc tacgaagcct ttccgggcga tctgtaggaa
     3781 ctcctacgaa gcctttccga gttgtctgta ggaactccta cgaaaggctg tcccagttgg
     3841 tccgtaggaa ctcccacaag gctttccggg tgaatctgta ggaactcctg cgaagccttt
     3901 ccgggttggt cagtaggaac tcccacgaaa gctttccggg tgaatctgta ggacctccca
     3961 aaggctttct gggagtgttc agtaggaact cctacgaagg ttttccaggt tggtctgtag
     4021 gaactcctac gaagactttc cggatggtca gcaggaactc ctgcgaaggt tttccaggtt
     4081 ggtctgtagg aactcctacg aagcctttcc ggggttgtct gtaggaactc ccacaaggct
     4141 ttccgggtga tccgtaggaa ctcctacgaa ggttttccag gttggtctgt aggaattcct
     4201 acgaaggttt tccggtgtgt ctgtagggct cctgcgaggc attccgggcg atctgtaggg
     4261 ctcctacgaa gcctttccga gttgtctgta ggaactccga caacccttat ttccggcttg
     4321 cagccttgtt ttggcacggc gaaactggtc aggtgaaacc tttgccagtc ggaatccaga
     4381 cgtttaaaga catacgtgat ggaaattatc tctacgtaga caagaccagg tggatttacg
     4441 agatg
//
LOCUS       DAOQGJ010000066         4433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10509_length_4433_cov_3.333029, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000066 DAOQGJ010000000
VERSION     DAOQGJ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10509_length_4433_cov_3.333029"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PK683_01750"
     CDS             complement(<1..337)
                     /locus_tag="PK683_01750"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07197.1"
                     /translation="MKKLPLGIQTFSKLAEGSHYYVDKTEYIRKLADIGTYFFLSRPR
                     RFGKSSFLDTLKEAYEGNENLFKGLYLEKNWDWSKKYTVIKISFGGGQTKNLDEFKIH
                     LQDIFLAFET"
     gene            complement(385..3228)
                     /locus_tag="PK683_01755"
     CDS             complement(385..3228)
                     /locus_tag="PK683_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNH07198.1"
                     /translation="METLLQYTFDFNLRTPDYKSGYLYLKGKEHLGLGRIVGQADGEY
                     EVNFSGNEDAVFLSEKDESIVHLEFEPLSLYKPESHVQEMELALSATELRLAHRFNKL
                     SSLSNSRTRLLPHQIESTYIVANSLRPRFILADEVGLGKTVEAALIMKELIFRKGYKT
                     VLIVTPSPLQVQWKQELKNKFNEDFDIVRKRNFVPGDTHWKKYSKIITSIDFIKNPKY
                     AEEILSKKWDIVIFDEAHRLRRDYHKVTRSYLFAEKISKITECLLLLTATPFRGKLEE
                     LYYIMHLVDPNILGSYHSFVNDYIVGNKTDLKEKISKVLLRRRKVEVGGFTKRFAKTV
                     KIELSDSERQFYEETTDYVKREYNLAMNTKNRAIGFVMVVFQKLLDSSVYALLSALSK
                     RKFMLEGRLHRFQGTRNSLEDWDSEETENIEDFIGELDEAAWPDLAGMRRELLTLNRL
                     ILLGKNIREDRKSQKLKDTLLKLKREGHKKFIIFTQFRTTQDYLTALLENDFTVTSFH
                     GSLSMEAKEVAILEFKNRTEVLICTEAGGEGRNLQFSNILFNYDLPWSPLKIEQRIGR
                     IHRFGQKDNVYIFNFACKDTVAERVLEVLTNKIRLFEESIGASDALLGTIEDELDFNS
                     SLMKFVTGVKTKVEIEQEIDSRIKVAEAGFKRLDSLVTPKLVDFNLQDYYSFTLDERQ
                     YQNTHLEEFILAFDRNYPEISGIDIEPAEKNIYRIRTGMDWKLATFDSELALKDDSLD
                     FLAFGHPIVENAIRYFQELRAGYKRKNILDSRFAGKIYFVFLAHFDFDLKRTEIFYYE
                     FDRNSGRTLSIEKIPEGAVSAPNAPVSSGRFPVEAALEYALIKTSEILKIDSEKRKDE
                     IHKETVSLFRKEEYKLEKSEEKAIRQLEEKLDRQEAVSKWNPSADRGHVISRTKNEIL
                     KTRENFEKELRKIRSGSNIRISLELFQVYCGTY"
     gene            complement(3229..3948)
                     /locus_tag="PK683_01760"
     CDS             complement(3229..3948)
                     /locus_tag="PK683_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNH07199.1"
                     /translation="MSEPSVHISEIFYSLSGEGISAGVPTVFVRLSGCSLRCGVTKDG
                     RKLWCDTPYALGPNTGEIMNISEVIDRIESVGASGTQILLSGGEPLEGADKREFCALF
                     SERVWKKRHNSLFSSPRVETNGREPISGLEYMVFTLDYKLPGSGMEGEMLLENFSVLR
                     ERKNSLDEIKFVVRDRKDFERSLEVIDSYQLQKMNLLFSPVEKECSAADLAEWIKTAG
                     IPNSRLSLQLHKILWGNKRGV"
     gene            4127..>4433
                     /locus_tag="PK683_01765"
     CDS             4127..>4433
                     /locus_tag="PK683_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07200.1"
                     /translation="MAKKIVKCPEWLKSNAVKSIYAVSSCVSKDFADYIPFWRHNQFW
                     FFDSVGIIQDVSREHRIDLSECEFLYYEIYESEFDDKLGEWVSFQAEDSFGYDVMIPE
                     "
BASE COUNT         1185 a          955 c          846 g         1447 t
ORIGIN      
        1 gagtttcaaa agccaaaaaa atatcctgca gatgaatttt aaattcatcc agattttttg
       61 tctgaccacc gccaaagctg attttgatga cagtatattt ttttgaccag tcccagtttt
      121 tttccagata caatcctttg aacaggtttt catttccttc gtatgcttct ttcagggtat
      181 ccagaaaaga actctttccg aaacgtcttg ggcgggacag gaagaaataa gttccaatat
      241 cggctaattt tctgatatac tctgttttat ccacatagta atgactgcct tctgcaagct
      301 ttgagaacgt ctgaatgccc aagggaagtt ttttcataag tacagactca gtatttcaga
      361 aaaggataca gtgaacagtc tttttcagta tgttccgcag tacacctgaa acagctccag
      421 actgattctg atattgcttc cggatctgat ttttctgagt tccttttcga aattttctct
      481 tgtcttcagt atttcatttt ttgttctgct gatcacatga cctctgtctg cagaaggatt
      541 ccatttggat acggcttcct gtctgtccag tttttcttcg agctgccgga ttgccttttc
      601 ctcggatttt tcaagcttgt attcttcttt tctgaaaagg gatactgttt ctttatgaat
      661 ttcgtctttt cttttttcag aatcgatctt taaaatttct gatgttttaa ttaaggcgta
      721 ttctaatgca gcttccaccg gaaatcttcc tgaactgaca ggcgcattcg gtgcagagac
      781 cgccccctcc ggaatttttt caattgacag tgttcttccg gaatttctgt caaactcata
      841 ataaaatatc tctgtcctct tcaaatcaaa atcaaaatgt gccagaaaca caaagtagat
      901 tttccctgca aatcgggaat ctaaaatatt ttttcttttg tagccggcac ggagttcctg
      961 aaaataacgt attgcatttt ccacaattgg atgcccgaaa gcgagaaaat ccagtgagtc
     1021 gtcttttaat gccagttcag aatcaaaggt tgcgagtttc cagtccattc ctgttcttat
     1081 cctgtaaata ttcttttctg caggttcaat gtctattcct gaaatttccg gatagtttcg
     1141 gtcaaatgca agtatgaatt cttccaaatg agtgttctga tactgtctct catccagagt
     1201 aaagctgtaa tagtcctgca gattgaaatc taccagcttg ggagtgacaa gtgaatccag
     1261 tcttttgaac cctgcttcag ctaccttgat tctggaatca atctcctgtt caatctcaac
     1321 ctttgtcttt acacctgtta caaatttcat taaggaagaa ttaaaatcca gttcatcctc
     1381 tattgttccc agaagtgcat ctgatgcgcc gatggattcc tcaaagagac ggatcttgtt
     1441 tgtcagcact tccagaactc tttctgcaac agtgtccttg caggcgaaat tgaagatgta
     1501 tacattgtcc ttctgaccga atctgtgaat ccttccgatt ctctgttcta tcttcagagg
     1561 actccagggc agatcataat taaaaagtat gttggaaaac tgaagatttc tcccttcacc
     1621 gcctgcttcc gtgcatatca gaacctcagt cctgttcttg aattcaagga ttgccacttc
     1681 ctttgcttcc atactgagag aaccgtgaaa tgaagtgaca gtgaaatcgt tttcgagaag
     1741 tgcagtcaga taatcctgag tggttctgaa ctgagtgaat atgatgaatt ttttgtgtcc
     1801 ttctcttttc agcttgagca gtgtatcttt cagtttctga gattttctgt cttccctgat
     1861 attctttcca agcagaatca gacggttcaa tgtaagaagt tctcttctca ttccggccag
     1921 atccggccag gcagcctcat ccagctcacc gatgaagtct tcaatattct cagtctcttc
     1981 agaatcccag tcttcaagag aattccgtgt tccctgaaat ctgtgaagcc ttccttccag
     2041 cataaatttt cttttgctga gagctgagag aagtgcatag accgaagagt cgagaagctt
     2101 ctgaaataca accatcacga aaccgatagc gcggtttttt gtgttcattg ccagattgta
     2161 ttctcttttt acatagtcag ttgtttcttc atagaactgt ctttctgaat cagaaagttc
     2221 tatttttaca gttttggcaa aacgttttgt aaatccccct acttccactt ttcttcgtct
     2281 gaggagaacc ttgcttatct tttccttcag atcagtttta tttccgacta tataatcatt
     2341 tacaaaagaa tgatatgatc cgagaatatt cggatctact aagtgcatta tataatataa
     2401 ctcttcaagt tttcctctga acggagtggc agtaagcagg agcagacatt cagtgatttt
     2461 ggaaattttc tctgcaaaca gataggagcg ggtcactttg tgatagtctc ttctcagccg
     2521 gtgagcttca tcaaaaatga caatgtccca tttcttggaa agaatttctt ctgcatattt
     2581 cgggttcttt ataaagtcta tggacgtaat gattttcgaa tattttttcc agtgagtgtc
     2641 tcccggaaca aaatttcgtt ttctcacaat gtcaaaatct tcattgaact tgttcttcag
     2701 ctcctgcttc cactgtacct gaagaggaga tggagtgaca atcagtactg tcttgtagcc
     2761 tttgcggaag atcagctctt tcataatcag agcagcttcc acagtcttgc cgagaccgac
     2821 ctcatctgcc agaatgaacc ttggccggag actgtttgcc acaatataag tggattctat
     2881 ctgatgagga agaagtctgg ttctggaatt ggaaagggag gagagtttat tgaatctgtg
     2941 tgcaaggcgg agctctgtcg cagaaagtgc aagttccatt tcctgtacat gactctccgg
     3001 cttataaagt gaaagaggtt caaattccag atgaacaatg gattcatcct tttcagacaa
     3061 gaatactgca tcttcatttc cggagaagtt gacttcgtac tcgccgtcag cctgaccgac
     3121 gattcttccc aggccaagat gttcttttcc ctttaaatag aggtatccgc ttttgtagtc
     3181 aggggttctt aaattgaaat caaatgtgta ctgaagaagt gtttccaatc agactcctct
     3241 tttatttccc cagagaatct tatgaagctg aagtgagagc cgtgaattcg gaatacctgc
     3301 tgtttttatc cactctgcca gatctgcggc actgcattct ttttcgacag gcgaaaaaag
     3361 aagattcatt ttctgcagct gataagaatc tatcacttcc aaactccttt caaagtcttt
     3421 tctgtccctc acaacaaatt taatttcatc cagactgttt tttctttccc gaagcacaga
     3481 aaaattttcg agaagcattt cgccttccat acctgagcca ggaagtttgt agtccaatgt
     3541 gaataccata tattcgagac cggaaatcgg ctctctgccg ttggtctcaa ctctgggact
     3601 tgaaaacagt gaattatgtc tctttttcca gacccgctcg gaaaataaag cacaaaactc
     3661 tcttttatct gcaccttcaa gaggctcccc tcctgatagc agaatttggg ttccagaggc
     3721 tccaacgctc tcaatgcggt caattacctc cgaaatgttc ataatttcgc ctgtgttcgg
     3781 tcctaatgcg taaggagtat cacaccagag ttttctgccg tccttggtca caccgcatct
     3841 cagactgcat ccggaaagcc tgacgaagac tgtgggaact cctgcactga tcccttctcc
     3901 tgaaagagaa tagaatattt ctgaaatgtg tactgagggt tcagacataa atactgtatc
     3961 ggaatacttt caatgcgaca taagaaacaa aaagcttttc tgtcaagaga aaaaacaact
     4021 gtgattctgc agatactcaa taaacagaat caaaatcagg tctgaataaa ttctttactt
     4081 tagatagatg tctccatata attcaaatat gactcctgcc ggatatatgg ccaaaaaaat
     4141 agtgaagtgc ccggagtggc ttaagtcaaa tgctgtcaaa tccatctatg ccgtgagttc
     4201 ctgtgtttcc aaggattttg ctgactatat tccgttctgg cggcataatc agttctggtt
     4261 ttttgattct gtcggaatca ttcaggacgt ttccagagaa catcgaattg atctttcaga
     4321 gtgtgagttc ctctattatg aaatttacga atcagagttt gatgataaac ttggggaatg
     4381 ggtttctttt caggcagagg acagttttgg atacgatgta atgattcccg aaa
//
LOCUS       DAOQGJ010000067         4414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10582_length_4414_cov_3.620096, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000067 DAOQGJ010000000
VERSION     DAOQGJ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_4414_cov_3.620096"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..944
                     /locus_tag="PK683_01770"
     CDS             69..944
                     /locus_tag="PK683_01770"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNH07201.1"
                     /translation="MESNSGSFLKKRICIVTDLDGTLLNSESEFPEKNRNTLQNFLNH
                     DHIIIFATGRRFHSTSRYAAELKGNVYLVCNNGQVTGVCGEKKEIWDTNFLDSEMSRT
                     ASEIGKSRGLSPILHTGMFEEGIDMSMESGKGSEAYVHNNKDRIFFSESHHDFPFARG
                     LVLCFLSKDMNTLKSLDREITDSLSEHSFRTVLTTINRVGPCLEILPKETSKWSGVRS
                     ILKKIQSHDIPVISFGDESNDIELIENSHIGIAPSNAVEPVKNIADRVSHLSNNEEFI
                     HYELEKILSSAALFM"
     gene            complement(945..1364)
                     /locus_tag="PK683_01775"
     CDS             complement(945..1364)
                     /locus_tag="PK683_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH07202.1"
                     /translation="MNQYLLDTNVISELPRRNPNQGAIDFLESLDEIVISAVTVDEIE
                     FGIQRTEKKQKQMLLKWWNALLSIPPIIIPVDETVAKTAGRLRAKEESRGRKMTQADA
                     LIAASALSTGRILVTRNWKDFKNSGVGLLNPFTGERG"
     gene            complement(1361..1642)
                     /locus_tag="PK683_01780"
     CDS             complement(1361..1642)
                     /locus_tag="PK683_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNH07203.1"
                     /translation="MKLESHFSVAEAKSRFSEVLHLAKDLPQFISSRGKEIGVILSRE
                     EYDKLMSDSRQNQPVSKLKNFLKLSRTIREENDLQIKIPRRKNRESVEF"
     gene            2078..>4414
                     /locus_tag="PK683_01785"
     CDS             2078..>4414
                     /locus_tag="PK683_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNH07204.1"
                     /translation="MKKLLSITLLLCLLVGTDSVRAEKKGKRSMKENFTNSAYESDWK
                     AVQEEEGKGLPKSALKLIDKIKQKAKEENNPPQYIKAMIFRLKNLSQVEEEEFIKAIN
                     STKIEALTAEEPTASFLHSMLAEMYWRYYELNRYRFLNRSKTVNIKLDDIRTWDLSKI
                     AAECIKEYEASLKTKEDTEKVSLNLFKEIIYNGSNNNDLSPTLYDFLLHRALNFYRNS
                     ESSLPVPKDEFRIDRKTISEAGSRKRNLNQMLYFAEKEKFVQMEIITAHRESFDYRAA
                     KLYQELLKFRMSRLNSPAEGEDSATSLMLADLERLHFVYLKTNDPSKESDYLAALEKL
                     SAISDKSAGLASHKIAEYYMNQGRNFNPNFPEKWKWANRTADQICDKVIASYPNSYGA
                     QNCSSLKEQLRVKELSVSMEGSVVSEKPFPAEIRYRNMEKVYWRIYKTSEKDITSVRE
                     EYEANNKKSIYKDYNRMLIDYFRKKKADYELTDSLPNDGDRHIHKTETKLPSLPTGEF
                     LVIACSSPDANYEKNAVYASQVKSTRLSYIHRDGKDYKEFYILDRETGEPLEGVKASL
                     VYWDYSSLRGKYVQEKGDSYKSDKNGYLEIPLHEKNYRQYFIKLKKENDELEIDNDNP
                     FSYYSYPALFYKTQKYNYSPSAQTRTFFFTDRAIYRPGQTLHFKGLLVQTNNEKSEIV
                     KNTEVYVQLMNANYQKAGELRLKTNEYGSYSGTFILPNDGLNGQFHIQETRYGGSQHF
                     SVEDYKRPKFEVKIETPKDSFRLGSKITVKGKAQSYAGA"
BASE COUNT         1438 a          917 c          923 g         1136 t
ORIGIN      
        1 gtgatcagga tatttcttgt ccttctactt cctcaggtag gaagcacaat cggaacatat
       61 gtggtgacat ggaaagcaat tcaggctctt ttctgaaaaa gagaatctgc atagtcactg
      121 atctggacgg gacacttctg aattcagaat cggaatttcc cgaaaagaac agaaacactc
      181 tgcagaattt tctaaaccat gatcatatca ttatatttgc aacaggcaga cggtttcaca
      241 gcacttcccg ttatgctgcc gaactgaaag gaaacgtcta tcttgtatgc aataacggtc
      301 aggtgacagg agtctgcggt gaaaaaaagg aaatctggga tacaaacttc ctggattccg
      361 aaatgagcag aactgcatcc gaaatcggaa aatccagagg actgtctccg atcctccaca
      421 ctggaatgtt tgaggaagga attgatatga gcatggaaag cggaaaagga agcgaagcct
      481 atgtgcacaa caataaggac agaatatttt tttcagaaag ccatcatgac tttcccttcg
      541 caaggggact ggttctatgc ttcctgtcaa aggacatgaa tacactgaaa agtctggaca
      601 gggaaattac tgattcgctt tcagaacact ctttcagaac cgttctgact acgatcaaca
      661 gagtcggtcc ctgcctcgaa attctcccga aggaaacatc taagtggagc ggtgtacgaa
      721 gtatactgaa aaaaatacaa agtcatgata ttccggtcat ctccttcgga gacgagtcaa
      781 acgacattga actgattgaa aattctcata tagggattgc cccgtccaac gcagtggagc
      841 ctgtaaagaa cattgcagac agggtcagtc atctttcgaa caacgaagag ttcattcact
      901 acgaactcga gaagatcctt tcctctgcgg cactgtttat gtgatcatcc tctctcgcca
      961 gtaaaaggat tcagaagtcc tactccgctg ttcttgaaat ccttccagtt tcttgtgaca
     1021 aggattcttc ctgtagaaag tgcagatgct gcaatcagtg catctgcctg ggtcattttc
     1081 ctgcccctgc tctcttcttt tgcacgcagt cttcctgctg tcttggcaac tgtctcatcc
     1141 acaggaatga tgataggcgg aatggaaaga agggcattcc accatttcag aagcatctgc
     1201 ttctgttttt tttctgttct ctgtatcccg aattctattt catccactgt gactgcactg
     1261 atgacaatct cgtcgaggga ttcaagaaaa tcgattgcac cctgattggg atttcttctg
     1321 ggaagttctg aaattacgtt tgtgtccaga agatactggt tcaaaattcg actgactctc
     1381 ggttttttcg cctggggatt tttatctgaa gatcgttttc ttctctgatg gttctggaaa
     1441 gtttaaggaa atttttcaat ttgctgactg gctgattctg tcgggaatcg gacataagtt
     1501 tatcatactc ctcccgactg aggatgacgc ctatttcctt tcccctgctg gaaataaact
     1561 gcggcagatc ctttgccaga tgaagcacct ctgaaaaccg tgatttcgcc tcggcgacgc
     1621 taaaatgaga ttcgagtttc atatagtcac ctccaatagt cattatgact atagaaaata
     1681 gttttttcgt caagcttttt taaaaaaaat ctgccctgct ttttaaaaag cggcattcga
     1741 ctctgtccct aaacggagaa attgaagttg ttgaaaaatc aagaaaaaaa tagggattgg
     1801 gcatatgggg gacttgggaa aagctctgat ttaatgctag gctttaaaca aagagaatat
     1861 aatcaataaa attattgctc cgcgaacccc gcgcgagact catttttttt atgcttttac
     1921 acaactctga gaaatcatat tattgtaaac ttttcctgct ttttcagaca gactatttct
     1981 ccagtctaat aaaaggaacc tgaaaagact tgactgattc tattttctga tttccattgc
     2041 catactgcaa tgatttactg ttatccgagg ggctgccatg aaaaaactgc tttcaattac
     2101 tctgcttttg tgtcttctgg tcggcacgga ttctgtccgc gctgagaaaa aaggaaaacg
     2161 atccatgaag gaaaatttca cgaattccgc ctatgaatct gactggaaag cagttcagga
     2221 agaagaaggg aaaggactgc ctaagtctgc acttaagctg atagacaaaa taaagcagaa
     2281 ggcgaaggaa gagaacaatc cgcctcagta cataaaggca atgattttcc gtctcaagaa
     2341 cctttcgcag gtggaggaag aagaattcat caaggcaatc aattcgacta aaatagaagc
     2401 attgactgca gaagaaccca ccgcgtcctt tctccactcc atgctcgcag aaatgtactg
     2461 gagatactat gaactgaaca gataccgctt cctgaacaga tcaaagacag tcaatatcaa
     2521 gctggatgat atcagaacct gggatctttc aaaaattgca gccgaatgca taaaggaata
     2581 tgaagcatct ctcaaaacaa aggaagacac tgaaaaagta tcattaaacc tttttaagga
     2641 aatcatttac aatgggtcaa acaataatga cctgagtccc acgctttatg attttcttct
     2701 gcacagggct ctgaatttct acagaaactc agaatcatct ctgcctgttc caaaagatga
     2761 attcagaata gacagaaaga caatttctga agcagggagc aggaaaagaa atctgaacca
     2821 gatgctgtat tttgctgaaa aagaaaaatt cgtgcagatg gagataataa ctgcacatag
     2881 ggaatcattt gactacaggg cagcaaaact ttaccaggaa ctgctgaaat tcagaatgag
     2941 caggctgaac agccctgcag aaggagaaga cagtgcaact tcccttatgc ttgccgacct
     3001 ggaaaggctc cattttgtgt atctgaaaac aaacgatcct tccaaagaat ccgattatct
     3061 ggcagctctc gaaaaactct ccgcaatatc cgacaaatct gcaggacttg catctcacaa
     3121 aattgcagaa tactatatga atcagggaag gaatttcaat ccgaactttc cggaaaaatg
     3181 gaaatgggca aacagaactg cggatcagat ctgcgacaag gtgattgcgt cctatccgaa
     3241 ttcgtacggt gcacagaact gcagttcact gaaagaacag ctgagagtaa aggaactttc
     3301 agtctcaatg gaaggatctg tggtgtctga aaaacccttc cctgcggaaa tcagatacag
     3361 gaatatggaa aaggtatact ggcgtatata caaaacatct gaaaaggata taacatctgt
     3421 cagggaagaa tacgaagcaa acaataaaaa aagcatatat aaagattaca accgtatgct
     3481 gattgattat ttcagaaaga aaaaggcaga ttatgaactg acggacagcc tcccaaatga
     3541 cggagacaga catattcata aaacggaaac aaagctccct tctcttccga ccggagaatt
     3601 cctggtcatt gcatgttcca gtccggatgc aaactatgaa aaaaatgccg tatatgcaag
     3661 ccaggtaaaa tctacaagac tgtcatatat tcacagggac ggaaaggact ataaagagtt
     3721 ttatattcta gacagggaaa caggagaacc tttagaaggc gtcaaagcat ctctggttta
     3781 ctgggactac agttctcttc gaggcaaata tgtgcaggaa aaaggagatt cctacaagtc
     3841 agacaaaaac ggttatctgg aaattcccct gcatgaaaaa aactacagac agtattttat
     3901 aaaactgaag aaggaaaatg atgaactgga gattgacaac gacaatccgt tttcctatta
     3961 cagctaccct gctcttttct ataaaacgca gaaatataat tattctcctt cagcacagac
     4021 aagaacattt ttctttacag acagagcgat ctacagaccc ggacagactc ttcacttcaa
     4081 gggacttctt gtccagacaa ataatgaaaa gtctgaaatt gtaaagaata cagaagtata
     4141 tgttcagctg atgaatgcaa attatcagaa ggcaggggaa ctccgtttga agacaaatga
     4201 atacgggtcc tacagcggaa cattcatcct tcccaacgac ggcctgaacg gacagtttca
     4261 cattcaggag actaggtacg gcggatcaca gcatttttca gtagaagatt ataaaagacc
     4321 gaaatttgaa gtcaagatcg aaacacccaa ggacagtttc cgtctaggaa gcaaaattac
     4381 agtcaaggga aaggcacaga gttatgccgg tgca
//
LOCUS       DAOQGJ010000068         4399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10636_length_4399_cov_3.005755, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000068 DAOQGJ010000000
VERSION     DAOQGJ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10636_length_4399_cov_3.005755"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="dnaN"
                     /locus_tag="PK683_01790"
     CDS             complement(<1..902)
                     /gene="dnaN"
                     /locus_tag="PK683_01790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNH07205.1"
                     /translation="MKFSVKTSEIMKAIHNVEGIISSRDIKSLLSNIKIECAGDRISL
                     SATDLEISIRSRVNATIEKEGTYCIPAKQLSNVIKVVNFTETRLETVQEEDTFQTFVT
                     DAENKVDFKMSINGYQGEELKTITETSDRDLVDFPCFTLSSMLKKTFYSVAVEDTRYV
                     FNGIYLKSTDNKITAVGTDGRRLAKLEREVPNKLPFTKGVIVPHKAIREILKLIESAS
                     DGKIGLIENQIYVSVNENALQCKLIDGAYPDYESVIPKESKYKVKLSKDAVQTALRQA
                     LIAAEEPIKQIRLTFETNLLRVNTS"
     gene            1989..>4399
                     /locus_tag="PK683_01795"
     CDS             1989..>4399
                     /locus_tag="PK683_01795"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNH07206.1"
                     /translation="MKNKYLFVLLTLCISVGCKGRSSHSPLSSLWPLALQGTDKKSAV
                     NSAENPPSNLTFTSSATGTNSSVLTVGVSVSIVPTFSGTVTNCTISPALPAGLTLNPA
                     TCAISGIPASSFPQTTFIITAGNQYGTATASLTLKGTADPPSNLSFPAGSSATFTTGT
                     YSSLVPTFTGGITDCRVSPALPAGIILNPSNCAISGTPTAVSPQTNYTITAGNQFGTA
                     SVVLNFGINTLAPQNLKYSFGASVSVNSNTTVNYTPGVTGGITGCSISPALPAGLIFN
                     TGNCGITGVPAVSSSGSYTVTAFNQYGNTDFTFALTTILTAPSAFSFPYSPGVTTVYT
                     IGTPVTVSPAITGVVTNCTASPSLPAGLSLDPNNCSITGNPSAVSGAVTYTITASNSA
                     GTVSTSIKLQVNNVPPSNLVYPFGASTVLAVGTAVSISPALNGGAASCTSSPALPAGL
                     SLNAANCTLSGTPSAAKAAALYTITASNAYGSTNTAVTITAQLYAPSSLSFPFSGGTV
                     NFTTGTAVNVSASVSGVVSSCTVNPALPSGLSLNASTCSITGTPNAVKASANYTITVS
                     NASGNISTTVTIGVNPPPPSSLVYPSGGNLSFAVGTAKTVTPTLTGTVTNCVSNPSLP
                     SGLTLNSVTCAISGTAAAEQGPSPYTVTASNSGGNTTANITISVAQPPLNLSYPAVST
                     LGYNNGVPISISPAVTGSSISYSISPSLPAGISLNASTGVISGTYSENNGSTASYTIT
                     AANSGGSITTNVTLTFFGKPPYKTGQTQCYDVSGNVISCSGTGQDGEHQYGQAHAIYS
                     GPNQHS"
BASE COUNT         1208 a          973 c          848 g         1370 t
ORIGIN      
        1 gatgtattga ctctgagaag gttcgtttca aatgtcagcc tgatctgctt gatcggttct
       61 tcagcagcaa tcaaagcctg gcgaagagca gtctgaacag catccttgga aagtttaact
      121 ttatattttg attctttcgg aatcactgat tcataatcag gataagcacc gtctatcaat
      181 ttgcactgaa gtgcattttc atttacagat acataaatct gattttcgat gagaccgatc
      241 tttccgtcag atgcagattc aatgagtttc agtatttccc tgattgcctt atgcggaaca
      301 atgacgcctt ttgtgaaagg gagtttattg ggaacctctc tttccagttt tgcaagcctt
      361 ctcccgtcag tccctacagc tgtaatttta ttatcagtag attttaaata tattccatta
      421 aaaacataac gtgtatcctc aacagcaacg gaataaaaag ttttcttaag catagaacta
      481 agagtaaaac agggaaaatc caccagatca cggtcggatg tttcagtgat tgtcttcagc
      541 tcctcgccct gataaccgtt tatggacatt ttaaagtcca ctttgttttc cgcgtctgtg
      601 acaaaagtct gaaaagtatc ttcttcctga acagtttcaa gtctggtctc ggtaaagttg
      661 actaccttga ttacattgct cagctgtttc gcagggatgc agtaagtgcc ttctttttca
      721 atggttgcat ttaccctgga acgaatggaa atttccagat cagtagcgga aagggaaatc
      781 ctgtcacctg cgcattcaat cttaatattg gaaagaagtg atttgatgtc tctgctagaa
      841 atgattcctt ccacattatg aattgctttc atgatctcgc tggtttttac actgaacttc
      901 ataagaatct ccctttatat tttaatatag tacttattta tttgtactat tatgtcctgt
      961 aactaagtta ataactctaa aattcacagt aaattcaaag aaatatgtct catacttttt
     1021 tgaaaataaa atttttaaaa gcgtcaagga aatttgctta tgagacgtaa ttaacggatt
     1081 atgtcttata aacagtttat ttaagtttat tttacttttt ataaacagtt tattgagatg
     1141 taaataaact gtttactcac tcaccgtacg cctgcggttg caggtggggc gtttcaggaa
     1201 cgccttccgg cggcttgagg gctcgcacaa atggaagctg ttgcaaaatt aaaatcggct
     1261 gccaacattt agaagctggt taactcctgc aagcttggaa gatgctgttt ttactgattc
     1321 atcgcaagct gttaaactgg tggatttccc taatgttttc tgaaaattac tgcgtctaga
     1381 cagcatatcg tatttaccta cggtgagagt tcgtttattt cagaaagtat cctgagggat
     1441 tttctgtata aaatttcagt ctagttgtaa aatgacctgc tttcttcttt cttgaaaatt
     1501 tgtctttaga tcaggcagag atttataatt tccgtaggtt tccttgaaaa acgatcaaat
     1561 tcggaaaatt tcctattata taaaagatca acttttatgc aaaaaggaaa aaatccggaa
     1621 tactctcatt gagcatatta cagaaggaat tgaggattgc atagactttc agattttttt
     1681 ttcaggagct tcagcatttt tgatctgttg taaccgttat gcattatttt ttaattattt
     1741 tgtatctgtt tggacgcgtg cttattcttt atttttgctg atcaccggga ataattcggc
     1801 tttcttgctg aaaatcttga ttttactgac tcaaatgggt tatgtgcaga aactattgaa
     1861 tatcagtatg ataaaacagt aaaaaaagca taatttcatc aaaaaaatat tgatttttcg
     1921 ctttgcattc aaaaagttat ttataggctg ctgcttcgtt aaatgcagca tattaaaaag
     1981 aggaatatat gaaaaataaa tatcttttcg ttctgctgac cttgtgtatt tcagtcggct
     2041 gcaaaggaag atccagccac tccccactct cctcgctatg gcctttggca ttgcagggaa
     2101 ctgataaaaa atcagcggtc aactctgcag aaaatccgcc ttctaatctg acatttacct
     2161 cttcagcgac aggtacgaat tcttccgttc tgacagtggg tgtttcagtg agtatagttc
     2221 cgacttttag tggaacagtg acgaactgca cgatcagtcc cgcacttccg gcagggctga
     2281 ctctgaatcc tgcaacttgt gcaatttcag gcatcccggc ttcatctttt ccgcagacaa
     2341 catttattat tactgccggc aatcagtatg gaactgcaac agcttctttg accttgaaag
     2401 gaacggcaga ccctccgtcc aacctgagtt ttcctgccgg ctcatcagca acatttacaa
     2461 caggaacata cagttcactg gtgcctactt ttacgggagg aataactgac tgcagagtca
     2521 gtcctgcact gcctgctgga atcattctga atccgagcaa ttgtgcaatc tcaggaactc
     2581 ccacggcagt ttcaccgcag acaaattata cgatcactgc aggcaatcag tttggaactg
     2641 catcagtcgt tttaaatttt ggaataaata ctctggcacc gcaaaatctg aagtattctt
     2701 ttggagcatc agtttcggtg aattccaata caaccgtcaa ttacacacct ggagttacag
     2761 gcggaatcac cggatgttcg atcagtccag cattgcctgc cgggctgatt tttaatacag
     2821 gaaactgtgg tatcaccgga gtgcctgcag tatcttcttc cggcagttat actgttacag
     2881 cgttcaatca gtacggaaac acggacttta cttttgcatt gacaacaata ctgacagcgc
     2941 cttctgcttt ttcttttcct tattctccgg gtgttacaac tgtatataca attggtacac
     3001 cagtaactgt cagtcctgct attactggcg ttgttacaaa ctgtactgcc agcccttccc
     3061 tgcctgcagg attaagcctt gatccaaaca actgttcaat tacaggaaat ccatctgctg
     3121 tcagcggagc agtcacatat acaatcactg cttccaattc agcaggaaca gtaagtactt
     3181 ctattaaact tcaggtaaac aatgttcccc cttccaatct tgtgtatcct tttggagcct
     3241 ctactgttct ggcagtaggt actgctgtaa gcatatctcc tgcgttgaac ggaggagctg
     3301 cttcctgtac ttccagtcca gctctccctg cggggctcag tctgaatgct gcaaactgca
     3361 ctctttcagg aactccgagc gctgcaaaag cagctgcgct ttacacaatt actgcatcca
     3421 atgcatacgg cagtacgaat acagcagtga ctataacagc acaactttac gcacccagca
     3481 gtctcagttt tcctttttca ggcgggactg tgaattttac tacaggtact gctgtcaatg
     3541 tctccgcatc tgtatccggt gttgtttcca gctgcactgt caatcctgca ctcccttccg
     3601 gactttctct gaatgcctca acctgttcta ttacaggaac acccaatgct gtaaaggctt
     3661 ctgcaaacta tacaattact gtatccaatg caagcgggaa tatcagtaca actgtaacta
     3721 tcggagtgaa tcctccccct ccttccagtc tagtttatcc atcgggcgga aatctttctt
     3781 ttgctgtcgg aactgcaaag acagtgactc ccaccttaac gggaactgta acgaattgtg
     3841 tatctaatcc ttctttgcct tcaggcttaa ctctcaactc agttacctgt gcaatttctg
     3901 gaacagctgc tgccgaacag gggccttccc catatacagt tactgcgagc aattctggag
     3961 ggaatacaac agcgaatatt acaatttcgg tggcacagcc tccattaaac ctgagctatc
     4021 ctgccgtttc cactcttggt tataataatg gagttccaat cagtatcagt cctgctgtaa
     4081 ccggaagcag catttcttat tccatcagtc cttcgcttcc tgcaggaatt tcattaaatg
     4141 cttctaccgg cgtaatttcc ggaacataca gtgagaataa tggttccact gcttcttata
     4201 cgattactgc agcaaattca ggagggagca taacgactaa cgtcactttg acattcttcg
     4261 gaaaaccgcc gtacaaaaca ggacagacac aatgttacga tgtgagcggt aacgtaattt
     4321 catgttccgg gacagggcag gatggagagc atcagtatgg tcaggctcac gcaatttatt
     4381 caggaccaaa ccagcattc
//
LOCUS       DAOQGJ010000069         4396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10652_length_4396_cov_3.587422, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000069 DAOQGJ010000000
VERSION     DAOQGJ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10652_length_4396_cov_3.587422"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PK683_01800"
     CDS             <1..923
                     /locus_tag="PK683_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH07207.1"
                     /translation="VARNANNLWMNFNGTAGIWRKKCIEDAGGWEHDTLTEDFDLSYR
                     AEMKGWRFRYFKDIVCKAEIPAMISAYKSQQFRWCKGSIQTAMKLLPRIWNHGLNWKV
                     KAEAVVHLINYSVCPLMVLNILLTAPLLLMEYWTGFSFTDLPLTILFLTATLLSVGSV
                     GPLFFYAYSQKEIYPDWKSRLGFLPVMVMIGTGVSIVNTRAWIEAVLGIKSGFKRTPK
                     LRIENSADSLKERMKYTVPMDFHAILEIMMGVYCIFCIYLSIVTYKPFIIGFLVIYTA
                     GFFFVAFGTLREAFWKLQNQWKRSVQVETA"
     gene            1018..1296
                     /locus_tag="PK683_01805"
     CDS             1018..1296
                     /locus_tag="PK683_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07208.1"
                     /translation="MEKVYCANCIHCVVVRHFEAEQDKYILRVKCNKKKWSKRSGEEK
                     LYKYFTVARRMQSDCEFYQEMGDILPYIKNLKKELPIKDEIYLVKSPV"
     gene            1318..2502
                     /locus_tag="PK683_01810"
     CDS             1318..2502
                     /locus_tag="PK683_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07209.1"
                     /translation="MISFKSSQFFELKKKTDLVVPVPILNDNLKVTSDFFGEKLEPIG
                     DFPRRVKIGETVFSSKASKILSPVNGIAEIDEKKQVHFRIDGRLNFRAKFERKDYTFL
                     ELKDKLNALGIVSLDLPGKNVSSLLDEFDASEDTIVIFAPYTQENFFDYRALMQSKFK
                     QELEAFKANIKKIFHKAQFIDYQTEKLTPYSYPDGHPRYFLYKHCGMNIEGELPVKKI
                     LYLGPETVYHILNALYYDIPFHERILSVVVINRLGFQEGENRSYRVRNGTDMKEFLET
                     IQSECGYKYFTVNSFYEKQPVYEIGAEFVFDIYRHHSIIICEELFHEKGEGVCTDCND
                     CSYFCPVSANPRALLENDKSMFAKDICLSCGLCSVFCPSHIDFADRIRFQKNRVQENA
                     VS"
     gene            2489..3421
                     /locus_tag="PK683_01815"
     CDS             2489..3421
                     /locus_tag="PK683_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07210.1"
                     /translation="MPSHKVSLTNHGFRIVSDDFYIDLFSLLVLSSLSVLSLFTVPDQ
                     IFQFIFLSAASGGFYFLYRQKVGYLYSVSFFSHMLLFWLLCPPFARNWVSLTCAVIIS
                     FSLLWKGNPFREIYFPFSLFIILVSIAVSLVFVEGFHFIFPLKSSGYMPWFADENLIP
                     GSYQSGLCLSGPANFRYYSYLESLRAMLIFIPCISLLRQYTKKLDFFLLLIFFASALF
                     IFRFDSSLSQERAVTLSCIWFLLFSMPGKNYGFSYIYSGFSLIISTVFSILLFHYYPE
                     LPPFIIVLNFFLCQSMFFVFVRQKNLKRMNRQKQ"
     gene            3418..3882
                     /locus_tag="PK683_01820"
     CDS             3418..3882
                     /locus_tag="PK683_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNH07211.1"
                     /translation="MNHLLELVTRSNIVYNLQAESKEEVIRKLVEHACSIRLVSSEKA
                     DEIVYLLLEREKSMSTGIGSGVAIPHCSITGMNELKVVIGLSKEGISFEAIDKLPVHI
                     FIMLIVPKEKFQDHIRTLALIAKTLNIREEREKFIHCQCYEEIISAFSAENK"
     gene            3886..>4396
                     /locus_tag="PK683_01825"
     CDS             3886..>4396
                     /locus_tag="PK683_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07212.1"
                     /translation="MRVETPRERILSEVSLFIVYLFCLMFSISVLINLRSLDAAYSAA
                     ERIADSDLKTLKNKKSFSKEFLETFLSVSSLNFGETLSGENVSRHILERLEPTFFLAA
                     TAVGAGSFFGIFPVLFFSARNWSLGISLLRIMSEFILSTPVFTAALFLFIIFFQIFAL
                     FPPGGYVRWD"
BASE COUNT         1246 a          842 c          921 g         1387 t
ORIGIN      
        1 aggttgccag aaatgccaat aatctttgga tgaactttaa cggtactgca gggatctgga
       61 gaaaaaagtg tattgaagat gcaggcggat gggaacatga tactctcact gaggatttcg
      121 atctttccta cagagcagag atgaagggct ggagattcag atatttcaag gatatcgtat
      181 gcaaggctga aattcctgca atgatttcag cgtataaatc acagcagttc agatggtgca
      241 agggaagcat tcagactgca atgaaacttc ttcctagaat ttggaatcat ggactgaact
      301 ggaaagtgaa ggctgaggca gttgttcacc tgatcaacta ttccgtatgt cctcttatgg
      361 ttctgaacat acttctcacc gcacctcttc ttttgatgga gtattggact ggattcagtt
      421 ttactgacct tcctctgact atcctgtttc tgacagcaac tcttctgtct gtcggatctg
      481 taggacctct tttcttctat gcttattctc agaaagaaat ctatcctgac tggaagtcca
      541 gactcggatt tcttcctgta atggtaatga tcggtacagg tgtttcgatc gtgaatacca
      601 gagcatggat tgaagcggtt cttggaatca aatcaggatt caagagaact cctaaactca
      661 gaattgagaa cagtgccgac agtctgaaag aaagaatgaa atatacagtg ccgatggatt
      721 ttcatgcaat actggaaatt atgatgggtg tatactgtat cttctgcatt tacctgtcta
      781 tcgttacata taaacctttt atcattggtt ttcttgtaat ctatactgca ggatttttct
      841 tcgtggcttt tggaacactc agagaagcat tctggaaact ccaaaatcag tggaaacgtt
      901 cggtacaggt agaaacggct tgacatcagg cctgtcagat caaaattggt taaaaatgaa
      961 gccagaatct tggctgtaga gacgattatg catggaaatt tgaatcaaga tatcgagatg
     1021 gaaaaagtat attgtgcgaa ctgcattcat tgtgtcgttg taagacactt tgaagcggaa
     1081 caggacaagt atatacttag ggtgaagtgc aacaagaaaa aatggtcgaa gagatccggt
     1141 gaggaaaaac tttacaaata cttcactgtt gcccgccgta tgcagtctga ttgcgaattt
     1201 tatcaggaaa tgggagatat tcttccttac attaaaaacc tgaaaaaaga actgcctatc
     1261 aaagatgaaa tctacctagt aaaatctcct gtgtagtagg ttagggaaga agggctcatg
     1321 ataagcttta aatcttccca gttttttgaa ctgaaaaaga aaacagatct ggttgtaccg
     1381 gttccgattc taaacgacaa tctgaaagtc acctcagatt ttttcggtga aaaattggaa
     1441 ccgatcggag atttcccaag aagagtaaaa atcggagaaa ctgttttcag ttctaaggca
     1501 agcaagattc tttcgccggt caacggcatt gccgaaatcg atgaaaaaaa acaggttcat
     1561 ttcaggattg acggtcgcct taatttcaga gcaaagtttg aaagaaaaga ttacactttt
     1621 ctggagctga aggataaact gaatgctctt ggaattgttt ctcttgacct gcccggaaaa
     1681 aatgtatcca gtcttctgga tgaatttgac gcatccgagg acactatagt catttttgct
     1741 ccctatacac aggaaaactt ttttgactac agggcattaa tgcagagcaa gttcaaacag
     1801 gagcttgaag cattcaaggc aaacatcaaa aaaatatttc ataaagcaca gttcattgat
     1861 tatcagacag aaaaactgac tccctattct taccctgacg ggcacccgag atatttttta
     1921 tataaacact gcggcatgaa catcgaggga gaacttccag tcaaaaaaat tctctatctt
     1981 ggtcctgaaa ccgtttatca tatactgaat gcattgtact atgacattcc ttttcatgaa
     2041 agaatacttt ctgtagtagt catcaacaga cttggattcc aggaagggga gaacaggtct
     2101 tacagagtgc ggaacggtac agacatgaag gagtttctgg aaacaattca gtctgaatgc
     2161 ggatacaaat atttcacagt caattctttt tatgaaaaac agcctgtcta tgaaatcggt
     2221 gcagaatttg tctttgatat atatcgtcat cattccatca ttatatgtga agaacttttt
     2281 catgagaaag gagaaggggt atgcacggac tgcaatgact gcagttattt ctgtccggtt
     2341 tcagcaaatc ctcgcgcact tctggaaaat gacaaatcaa tgtttgccaa ggatatctgt
     2401 ctttcatgcg gactctgttc tgtattctgc ccttcacata tagattttgc agacaggata
     2461 cggtttcaaa aaaatagggt gcaggaaaat gccgtctcat aaggtcagtc ttaccaatca
     2521 cggattcaga atcgtatctg acgattttta tatagatctg ttttctctgc ttgtactgag
     2581 cagtctttct gtgctcagcc tttttaccgt tccggatcag atctttcagt ttatctttct
     2641 gtcagcggcg tccggaggtt tttattttct gtacagacag aaagtagggt acctttactc
     2701 cgtcagtttt ttttctcata tgcttctctt ctggctcctc tgtcctcctt ttgccagaaa
     2761 ctgggtgagt ctgacctgtg cagtcatcat ttcattcagt ctgctctgga aaggaaatcc
     2821 gtttcgggaa atttattttc cgttttctct tttcataatt ctcgtttcca ttgcggtgag
     2881 cctagttttt gtggaaggat ttcattttat ttttccgctg aaaagttccg gatatatgcc
     2941 ctggtttgct gatgaaaatc tgatacctgg aagttaccag tcgggtctct gtctttcagg
     3001 tcctgcaaat ttccgatatt attcctatct cgaaagccta agggcgatgc ttatttttat
     3061 tccatgtatt tcgcttcttc ggcagtacac aaaaaaattg gatttttttc tgcttctgat
     3121 tttctttgca tcagctctgt tcattttcag atttgattct tccctttcgc aggaaagagc
     3181 tgtaacactt tcctgtatct ggtttcttct tttcagtatg cctggaaaaa attacggatt
     3241 ttcatatata tattcaggat tcagtttaat tatttccact gtgttcagta ttcttctttt
     3301 ccattattat cctgagctgc cgccattcat tattgtgctg aacttttttc tttgtcagag
     3361 tatgtttttt gtatttgtca ggcaaaaaaa tcttaagagg atgaacaggc agaaacaatg
     3421 aatcaccttt tagaattagt aacaagatcc aatatcgtct ataatcttca ggctgagtca
     3481 aaagaagaag tgatcagaaa acttgtggag catgcctgca gtatccgtct agtgagcagt
     3541 gagaaggcag atgagattgt ctaccttctt ttggaaaggg aaaaatcaat gtctacaggg
     3601 atagggagcg gagttgcaat tccgcactgt tccattaccg gaatgaatga actgaaggta
     3661 gtcataggcc tttccaaaga agggatttca tttgaagcga tcgacaagct gccagtgcat
     3721 attttcatta tgctgattgt gccaaaagaa aaatttcagg atcatatcag aactctggct
     3781 ctgatcgcga agactctgaa tattcgggaa gaacgggaaa aattcattca ttgccagtgt
     3841 tatgaagaga ttatctcggc tttttctgca gagaacaagt gaaaattgag agtagagaca
     3901 cctagagaaa gaattctttc ggaggtctcc ctattcattg tttatctttt ctgtcttatg
     3961 ttttccatct ccgtgctgat caatctgaga agcctggatg ctgcgtattc agcggcggaa
     4021 agaattgcag attccgatct gaagaccctg aaaaataaaa aaagtttttc aaaggaattt
     4081 ctggaaacat ttctttctgt atcctcgctg aatttcggtg aaacactttc cggagaaaat
     4141 gtgtccaggc atatccttga aaggcttgag ccaacctttt ttcttgccgc gactgcagtc
     4201 ggagcaggtt catttttcgg aattttcccc gttctcttct tctctgccag gaactggagt
     4261 ctgggtatca gcctgcttag aattatgtct gaattcatac tttccactcc ggtgtttacg
     4321 gcagcactct ttctgtttat catatttttc cagatattcg cactgtttcc tcccggggga
     4381 tatgtcagat gggata
//
LOCUS       DAOQGJ010000070         4392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10664_length_4392_cov_3.896472, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000070 DAOQGJ010000000
VERSION     DAOQGJ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10664_length_4392_cov_3.896472"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PK683_01830"
     CDS             complement(<1..569)
                     /locus_tag="PK683_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07213.1"
                     /translation="MKYIIFYIICSFCFTAYSQELHKHPVPKEKKRGAKKLSELSGKA
                     LEKNEQPESEKNSETNNAAESQELKPEKTGEDTVPVSEDKSVPRKDIKTDPADQTEHH
                     HSDSKEHSHSQEPVSHSHGKVLGMPAGLMVPHIHGSKEWMIDYMYMSMDMNEMYVGGK
                     AQDPSKYLIGLQPNYSLSVSTSSGVHNHSG"
     gene            complement(580..1005)
                     /locus_tag="PK683_01835"
     CDS             complement(580..1005)
                     /locus_tag="PK683_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07214.1"
                     /translation="MKKTLLYLILAVSVCSGSACQEKKNSSKEAVLALLALSRSSAAG
                     SASSSCTAYTCSGTTTFSTVSSSGMSSGCAQSGCHSAAAQTSGFNATDYASAKSFTVP
                     GNACSSRLYSMVTAGSMAGNSNSALNKAIYCWIQGGSAQ"
     gene            complement(1329..2675)
                     /locus_tag="PK683_01840"
     CDS             complement(1329..2675)
                     /locus_tag="PK683_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07215.1"
                     /translation="MKLYYIMSAVLMLFQACTLDFSGSGKSGSKSKNTVMGVAALAIS
                     SQSDSFLMVPNSSADNTTMSLVSHKSGTNTVLSSIKFDSNAGDTVSTANDHFFTAIGS
                     LNKVAVLTINSMGAALEGYISTGTKPVHMYTDPDGKVWIMNDGNSGVDSINCSTSGTG
                     SVTIIKDGAKGTTGSSAASLIKHICVGKGHHKALFTSSPKRAFVSNISDATVTVIDND
                     SASSTYLTVLSTVSLVTGAGPHGFGYSSVSGKVYVANASLGTVTELNPSTLTTSIIST
                     VKSGPAAVSPDGKYAVFPGVDTSSDSNHVTGKLSVVNVSSTSSITLKDIADVSPDMMM
                     FSEDGKKLFVVSAQAGSGNQLTNLKQNVLLIYDSSALPTLTLLKEVTVGTANAGHRHF
                     DAMTHGSMTHLFIPSGDGNLYTVDADSMSVTQTLKIGGTFDNLHFHMLGMGSMHSH"
     gene            complement(2787..3233)
                     /locus_tag="PK683_01845"
     CDS             complement(2787..3233)
                     /locus_tag="PK683_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07216.1"
                     /translation="MSEKKTLKKISSVISILLLSFNILFQNLCYSGVLAANLICTCSH
                     LSESEKHSEDIFLSAHRKFSQENDLSLPECHRPKIKHSSHECHCKKGEERKRMISALF
                     QMNLIRNEIRFIFSVPERTFTEREQFNCRNLFLKHHFRPPKFSSVV"
     gene            complement(3468..3821)
                     /locus_tag="PK683_01850"
     CDS             complement(3468..3821)
                     /locus_tag="PK683_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH07217.1"
                     /translation="MNQNLLLLFGVIAAVYFVFVMVRSKKSIKEIRKVMEGKAKIVDV
                     RSPAEFAGGSYPGAVNIPVDQISARISEFGDKNGAVIVFCASGMRSARAKAMLDGAGF
                     TNVTNAGTLSNMMSA"
     gene            complement(3822..>4392)
                     /locus_tag="PK683_01855"
     CDS             complement(3822..>4392)
                     /locus_tag="PK683_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH07218.1"
                     /translation="NHAGYFPGAERLALKLVYEKKTGKLLGAQCFGGEGADKRIDTAA
                     TALASGMTVQEIAELDLAYAPPFSSANDPINFAAFQASNQLEGSVNIISASELDSLKD
                     FFFLDVRNPDEISRYKVNTNLQIKVDSLRDHINEIPKDRKIVCLCQSGQRSYIASRIL
                     VQSGFKDVVNLAGGYWTLSAYRKSVNKEN"
BASE COUNT         1235 a         1010 c          887 g         1260 t
ORIGIN      
        1 cctgaatgat tgtgcacgcc tgaactagta gaaacactga gactgtaatt gggctgaagt
       61 ccgatcagat acttggacgg gtcctgcgcc ttccctccga catacatctc attcatatcc
      121 atgctcatgt acatatagtc tatcatccat tccttggatc cgtgaatatg aggcaccatc
      181 agtcctgccg gcattcccag aactttccca tgactgtgac tgacgggctc ctgtgaatga
      241 ctgtgctctt ttgaatctga atgatgatgt tccgtctgat ctgcaggatc tgtttttata
      301 tcctttctcg ggacagattt atcttctgat acaggaacgg tatcctctcc tgtcttctcc
      361 ggtttcagtt cctgactttc tgccgcatta ttcgtttccg aatttttctc agactcaggc
      421 tgttcatttt tttccaatgc cttcccggaa agttcagaga gtttctttgc accccttttt
      481 ttctctttgg gaaccggatg cttatgaagc tcctgactgt atgcggtaaa acagaaagaa
      541 cagataatat aaaatataat atatttcaaa tgcattccct cactgagctg acccgccctg
      601 aatccagcag tatatcgcct tgttcagtgc tgaatttgaa tttccggcca tagaacccgc
      661 cgttaccata gaatacagtc tgctggaaca ggcattaccg ggaacagtga aagattttgc
      721 agatgcataa tcagtcgcat tgaaccctga ggtctgcgct gctgcactgt ggcatcccga
      781 ctgggcacag ccagaagaca ttccggatga gctgactgtc gaaaatgtcg ttgtgccgga
      841 gcaggtatat gctgtgcacg aagaagatgc agaaccggct gcagacgatc tggacagggc
      901 caggagagca agtacagcct ccttcgaaga attctttttt tcctgacagg cagaacctga
      961 acagacggat acagccagaa tcagataaag cagtgttttt ttcatgaatt atcctttaat
     1021 tattttttaa atacatactg atacaaacat ccttcctgtt caatccggcg ccgcacagac
     1081 tgagggaagg agatacctta aagaatctgt cacctgaata ctttaacatt agacttgctg
     1141 atatcagagt cagtctgtcc tgaatttatc gaaggaagcc atgaagtcag catccctcaa
     1201 aacacaaaac tcattgcagt gctcagaatg agattgacac aaattcaaga gcaagtctat
     1261 ttctcattta cggaaaagta cagaagatgt ctgcgctttt cctttacata tccggagcag
     1321 tttctgactc aatgagaatg cattgatccc attcccagca tatgaaaatg aagattatca
     1381 aatgttccgc cgatcttcaa agtctgagtc acactcatgg aatcagcatc cactgtatac
     1441 agatttccgt caccgctcgg aatgaaaaga tgagtcattg atccgtgagt cattgcatca
     1501 aaatgtctgt gccctgcatt tgctgttccg actgtcactt ctttcagaag agtcagtgtc
     1561 ggaagtgcag atgaatcata aatcagaaga acgttctgtt tcaggttcgt cagctgattc
     1621 cccgatccgg cctgtgctga tacaacaaac agtttctttc cgtcctcgct gaacatcatc
     1681 atatctggac ttacatctgc aatatccttt aatgtaattg aggaagtgct tgatacattc
     1741 acaacggaaa gttttccagt tacatgattt gaatcagaac ttgtgtccac accgggaaat
     1801 acagcatatt tgccatcagg cgataccgca gcaggtcctg acttcacagt actgatgatg
     1861 gaagttgtca aagtggaagg gttgagttca gtgactgtac cgagtgaagc gtttgctaca
     1921 tagactttcc ctgatacaga tgagtatccg aatccgtgcg gaccagcacc tgtcacaagt
     1981 gacactgtgc ttaagacagt aaggtacgtt gaactggcag aatcattgtc aatgacagtt
     2041 actgttgcat cggaaatatt gcttacaaaa gctcttttcg gactggatgt aaacagtgcc
     2101 ttatgatgtc cttttcccac acagatatgt ttgatgagtg aggctgcact gcttccagtc
     2161 gtaccctttg caccatcctt gatgatcgta accgacccgg ttcctgaagt actgcagttg
     2221 atgctgtcca ctccggaatt tccgtcattc attatccaga ctttcccgtc aggatcagtg
     2281 tacatatgga ctggttttgt acctgtgctg atataacctt ccaatgccgc ccccatactg
     2341 tttattgtaa gcacagcaac cttgttcaga gaaccgatag cagtgaaaaa gtggtcattt
     2401 gcagtgctta cagtgtcccc cgcatttgaa tcaaacttga ttgaggaaag aacagtattt
     2461 gtaccggatt tatgactgac aagactcatc gttgtattgt cagcgctgga attaggcacc
     2521 atcagaaatg aatctgactg tgaggatatg gcaagagcgg caactcccat aaccgtattt
     2581 ttggactttg aacccgattt gcctgatccg gaaaaatcca gagtgcaggc ctggaacagc
     2641 atcaatactg cagacatgat gtaatataat ttcatgataa tctccttgat tgtataagtc
     2701 tgaaagcctg taaccaggca ttatttttca gaactcaatt cagcaaaaaa cagagaatgc
     2761 agacagtctc tgcattctca gaatgcttaa acaactgagg agaatttagg agggcgaaaa
     2821 tgatgtttca ggaacagatt cctgcagtta aactgctccc tctcagtgaa agtacgttca
     2881 ggaacagaga aaatgaaccg gatctcattt ctaatcagat tcatctgaaa aagggccgaa
     2941 atcattctct tccgttcttc tcccttttta caatgacatt catgagaaga atgctttatt
     3001 ttaggccgat gacattcagg gaggcttaaa tcattttcct gactgaattt tctgtgagca
     3061 gaaaggaata tatcttcaga gtgtttttct gattcggaca gatgactgca ggtgcagatc
     3121 agatttgccg ccagtacccc ggaataacag agattctgaa acagaatatt gaaggaaagc
     3181 agaagtatgg aaatgactga actgattttt ttcagtgttt ttttttcaga cattctcatt
     3241 gtacagacat cctaacttat caatattttt ctgcattgac cgaggtcaag aaaaaagaaa
     3301 attatctgag tctcaaaatc aaaaaaatct gaaatataaa atattacaca gccttcctcc
     3361 agcaacacag tgaagagagc gaacccttaa aaaatgaaaa agccgaaatc agaaactgat
     3421 ttcggcttaa tgaagtctgg aaatacagac agatctgtct atcttttcta tgcggacatc
     3481 atatttgaaa gtgtgccggc attggtgaca ttggtgaaac ctgctccgtc aagcatcgct
     3541 tttgccctcg cacttctcat tccggaagca cagaacacaa tgactgctcc gttcttgtct
     3601 ccaaattcac tgatccttgc actgatctga tccaccggaa tattcacagc tccaggataa
     3661 cttccccccg caaattctgc aggtgaacgg acatctacaa tcttagcctt gccttccata
     3721 acttttctaa tctcctttat actttttttt gaacgtacca ttacgaatac gaaatatacc
     3781 gcagcgatga ctccgaacaa aagcagcaga ttctgattca tttaattctc cttgtttact
     3841 gattttctgt atgcactcag ggtccagtat ccccctgcca gattgaccac atccttgaat
     3901 ccgctctgca caagtatcct cgaagctata taacttctct gtcctgactg acagagacag
     3961 acaatctttc tgtccttggg aatttcattt atatgatcac ggagagagtc tactttgatc
     4021 tgaagatttg tatttacttt atatcgcgaa atctcatcag ggtttctcac atccagaaag
     4081 aaaaagtctt tcaaagaatc cagctcggat gctgaaatga tgttcactga accttccagc
     4141 tgattggatg cctggaatgc tgcaaaattg atcggatcat ttgcagagga aaaaggagga
     4201 gcataggcca gatccagttc cgcaatctcc tgaacagtca ttccggatgc aagagcggtc
     4261 gcagctgtat caattctctt gtcagccccc tctcctccga aacactgtgc cccaagcagt
     4321 ttccctgttt tcttttcata gacaagcttc agagcaagcc tttccgctcc tggaaaatat
     4381 ccagcgtgat tg
//
LOCUS       DAOQGJ010000071         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10684_length_4388_cov_3.057928, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000071 DAOQGJ010000000
VERSION     DAOQGJ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10684_length_4388_cov_3.057928"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PK683_01860"
     CDS             <1..672
                     /locus_tag="PK683_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07219.1"
                     /translation="AGTWKWRGGVLCPNGKIYGIPMNSTSVLVIDPATDTANTFGSVG
                     AAIEKWEGGVLAPDGKVYGIPRSETGVLVIDPATDTASTFGSLAGTWKWIGGAIAPDG
                     KIYGMSFDSASVLVIDPSMDTASTFGSVGAGGNKWAGGVIAPNGRIYGIPWNVTNVLE
                     IDTAANSTLAFGSVGSGYQGGILAPNGKIYGIPHTATSVLVIDSKSNGVWPSDLYLSP
                     FFNKH"
     gene            1021..1488
                     /locus_tag="PK683_01865"
     CDS             1021..1488
                     /locus_tag="PK683_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HNH07220.1"
                     /translation="MIYTLDYPNFEVKNAFLAHFAQLWTNKEAFNSYIHKLIQSLHGH
                     DFESFFTILRSIFANIDYDLHIPQEKYYQTVFYLIFTLIGLRVSAEVLVLSRVEAKTN
                     IGRIDAVIKDREIFLFEFKFSGTKEDALAQIKKNKILFQRNRHPVSVSLRCRI"
     gene            1584..4316
                     /locus_tag="PK683_01870"
     CDS             1584..4316
                     /locus_tag="PK683_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HNH07221.1"
                     /translation="MNQKSEQKARDLIDRQLEEAGWIVQDSDKMDFSAGAGIAFREMQ
                     TESGPADYVLFISRKPCAIIEAKKFGTILTPVEEQTERYAKSKLPRLRHYSAPIRFLY
                     ETTGLETHFTDTADPKPRAREVFHFHRPEALKELLEQDSTLRKKMKEFPKLSRENLRD
                     CQFTAIENLEKSFGENKPKALVQMATGAGKTFTAITSIYRLLKFANAKRILFLVDTKN
                     LGEQAEKEFQAYTPQDDKRKFTDLYVVQRLSSNSISAASHVCISTIQRMYSILRGDNE
                     LTLNEEKSLNEGSEISRPVEVKYNAKVPIEMFDVIIIDECHRSIYNLWKQVLDYYDAF
                     LIGLTATPDSRTFGFFNKNVVSEYTHQQAVADGVNVGYDVYTIETKITAQGAKIEAKQ
                     FIDKRDKLTRKMSWEQVDEEVEYAPTELDSKVVNPDQIRTVIKTFKEKINTEIFPNRK
                     EVPKTLIFAKTDSHAEDIIKIVREEFGEGNAFCKKVTYGNTEEKPSEVLQQFRTAYHP
                     RIAVTVDMIATGTDVKAIECLLFMRDVRSKNYFQQMLGRGTRTFSYDDLKRVTSSATG
                     NKTHFVVIDAVGVCKSIKTDSRPMERKPTVSMKKLMEDIVFGNTEEDTFSSLANRLSR
                     LERSLNQKEKNDLEKLSGESIHTTIGNLLAAFDPDELEAERRTMSSTATAEDARQKII
                     EKIHKTFDKPEYREKLFNIQKSHFQIIDRTNIDEVTSVGWDADAKKKSEALIIEFKDY
                     LKENKDKIIGLQIFYSFKKRKKELTFQIIQDLSEAILQAKPKFAPLNLWKAYEQLETV
                     EGKPEQELTALVSVIRRVLEIDSKLTSYDITVNKNFQRWIFAKHAGKPKYTEEQMEWL
                     RMMKDFIAVSIRLEKEDLAELPPFCDKGGLGRARKLFGTDLNQMLNELNEELAA"
BASE COUNT         1482 a          799 c          983 g         1124 t
ORIGIN      
        1 gccggaactt ggaagtggcg cgggggagtt ctttgtccca acggaaaaat ttatggaatc
       61 cctatgaatt ccacaagtgt actggtgatt gatccggcaa cagatactgc caatactttt
      121 ggttctgttg gagcggcaat tgagaaatgg gagggagggg tgctagctcc agatgggaaa
      181 gtttatggaa tcccaagatc cgaaacaggc gtattggtga ttgatccggc gacagatact
      241 gccagtactt ttggttcact tgcaggaact tggaaatgga ttggaggagc tattgcgccc
      301 gacggaaaaa tctatgggat gtcatttgac tccgcaagtg tattagtcat tgatcccagt
      361 atggatacag ccagcacttt tggctcagtt ggagctggcg gcaacaagtg ggctggcgga
      421 gttattgctc ccaacggaag aatctacgga attccatgga atgttacaaa tgttctggaa
      481 atcgacacag ctgccaacag cacacttgcc tttggaagtg tgggatcggg gtatcaagga
      541 ggaattcttg ctcccaatgg aaaaatctat ggaattcccc acaccgcaac aagtgtatta
      601 gtgattgatt ccaagtccaa cggtgtctgg ccttcagact tgtatttgtc accgtttttc
      661 aataagcatt gagttttcaa ttgtgattca tccactcaga ttcaaaagct gaaagaaaaa
      721 ggaacgttaa gtgtcttaat tcagcgatag ccggaattac agaagaagaa ttaaaaaaaa
      781 atttcaaaga atatctatta gaattttcta atacagaaaa aatttcagaa ccactggttc
      841 gaaacagcaa ctcccagttt cttaattgag cttgcaaaga agaaagattt ttctgtcagt
      901 gaactgccaa tagaagcaga tgaactgtct ttttcaacgt atgatgtaga aagtcttcaa
      961 attctcccgc ttttgtttca aactggttat cttaccttaa aaggatatga tccggaatct
     1021 atgatatata cattagatta tccgaatttt gaggtcaaga atgcttttct ggctcatttt
     1081 gctcagttat ggacaaataa agaagctttt aacagctata ttcataagct gattcagtct
     1141 ctgcacggac atgactttga atccttcttt acaatcctgc gctccatctt tgcaaacata
     1201 gactatgatc ttcatatccc tcaggaaaaa tactaccaga ctgtatttta tctgatcttt
     1261 acattaatcg gacttagagt ttctgcagaa gtacttgtgc tgagtcgtgt cgaagcaaag
     1321 acgaatatcg gccggataga tgcagtcatt aaagacagag agatctttct atttgaattc
     1381 aaattcagcg gaacaaaaga agatgcactc gcccagatca aaaagaacaa aatactattc
     1441 caaaggaaca ggcacccagt ctctgtatct cttcggtgta gaatttaaag ataagaatgt
     1501 tggggaatgg gtaatggagg aggttggctg aaaatttttt cgtgttgaca atagtatgtt
     1561 ttcctaaata aatatggtgt aatatgaatc agaaatcgga acagaaggca agagatctta
     1621 tagacaggca gttggaagaa gcaggctgga ttgttcagga ttctgataaa atggattttt
     1681 cagctggcgc aggcattgct tttagggaaa tgcagacgga atccggtcct gcagattatg
     1741 tattgtttat cagcagaaaa ccatgtgcca ttattgaagc caaaaagttt gggacaattc
     1801 ttacgcctgt agaggaacag actgaacgat atgctaaaag caaactcccc agattaagac
     1861 actattctgc accgattcgg tttctgtatg agacaaccgg cttggaaact cattttacgg
     1921 atacagccga cccaaagccc agagccagag aagtatttca ttttcacaga ccggaagcac
     1981 tgaaggagct gctcgagcag gattccacct tgagaaaaaa aatgaaagag tttccaaaac
     2041 tgagcagaga gaatttacgg gactgtcagt tcactgcaat tgaaaacctg gaaaaatctt
     2101 tcggtgaaaa taagccaaag gctcttgttc agatggccac aggagccgga aaaacgttta
     2161 ctgcaattac atccatctac agactgctca agtttgcaaa tgctaaaaga atactctttt
     2221 tagtggatac taaaaaccta ggcgaacagg cagaaaaaga atttcaggca tatactcctc
     2281 aggatgacaa aagaaaattc acagacttgt atgttgtgca aagactttcc tcaaattcaa
     2341 tttctgcagc atcacatgtc tgcatcagta caatacaaag gatgtactct atcctgagag
     2401 gtgataacga actgacactg aatgaagaaa aatcactgaa tgaaggttct gaaatcagca
     2461 gacctgtaga agtgaaatac aacgccaaag ttcccataga aatgtttgat gtcatcatta
     2521 ttgatgagtg tcatcgaagt atttacaatc tgtggaaaca ggtgctggat tattatgatg
     2581 catttctgat cggactgaca gcgactcctg acagccgtac attcggcttt ttcaacaaga
     2641 atgtcgtaag tgagtatact catcagcagg cagttgcaga cggtgtgaac gtgggatatg
     2701 atgtatatac gattgaaaca aagatcactg cgcagggtgc aaaaattgaa gcgaagcagt
     2761 tcattgacaa aagggacaag ctgacacgaa aaatgagctg ggagcaggtg gatgaagaag
     2821 tggaatacgc tcccactgaa cttgacagca aggtagtcaa tcctgatcag atcagaactg
     2881 tgatcaaaac ctttaaagaa aagatcaata ctgaaatttt tccaaacaga aaagaagttc
     2941 caaaaacttt gatttttgca aagacggaca gtcatgcaga agatattatt aaaatcgtca
     3001 gagaagaatt tggagaagga aatgctttct gcaaaaaagt gacatacggg aatacggaag
     3061 aaaaaccttc cgaagttctg cagcagttca ggacagccta tcatccgaga attgcagtca
     3121 ctgtcgatat gattgccacc ggaacagatg tcaaagcaat agaatgtctt ctgtttatga
     3181 gagatgtccg gagcaagaat tattttcagc agatgcttgg aagaggtaca aggacattca
     3241 gttatgatga tctgaaaaga gtcacttctt cagcaacagg aaacaagact cattttgtgg
     3301 ttattgatgc agtcggtgta tgcaagtcca taaaaacaga cagcagacca atggaaagaa
     3361 aaccgactgt gagtatgaaa aagctgatgg aagatatagt gttcggaaat acagaagagg
     3421 atactttcag ttcgcttgcc aataggcttt cccgtctgga aagaagtctg aaccaaaaag
     3481 aaaaaaatga tttggaaaag ctttccggtg agtctattca tactacaatc ggaaatctgc
     3541 ttgctgcatt tgatccggat gagctggagg cagaaagaag gacaatgtct tcgacagcga
     3601 cagcagagga tgcgagacag aagatcatag aaaaaattca taagacattt gataaaccgg
     3661 aatacagaga aaaacttttc aatatccaga aatctcattt tcagatcata gacagaacga
     3721 atatagacga agtcacttca gtgggctggg atgcagatgc aaaaaagaaa tcagaagcat
     3781 tgattataga atttaaagat tatctgaagg aaaataagga taaaatcatc gggctgcaga
     3841 ttttttattc tttcaaaaaa agaaagaagg aacttacttt ccagataatt caggatcttt
     3901 ccgaagccat tctgcaggca aaaccgaagt ttgccccgct taatctatgg aaagcctatg
     3961 aacagctgga aacagtggag gggaaaccgg aacaggagct cacagccctc gtatccgtca
     4021 tccgccgtgt gctggaaatt gattctaagc ttacatccta tgacatcaca gtaaataaaa
     4081 actttcaaag atggattttt gcaaaacatg caggcaagcc aaagtataca gaagaacaga
     4141 tggagtggct tcgaatgatg aaggacttca ttgcagtcag catccgtctg gaaaaagaag
     4201 atctggctga gcttcctccc ttctgcgaca aaggcgggct gggcagagca aggaaattat
     4261 tcggaactga tcttaaccaa atgctgaacg aactgaatga agagctggcc gcgtgaaaag
     4321 cataaatgaa tttttcaaag aaaattaaaa taaagacaga attattgaca aaaaatgcta
     4381 gttttttg
//
LOCUS       DAOQGJ010000072         4374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10740_length_4374_cov_3.578606, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000072 DAOQGJ010000000
VERSION     DAOQGJ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10740_length_4374_cov_3.578606"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PK683_01875"
     CDS             <1..765
                     /locus_tag="PK683_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH07222.1"
                     /translation="RQISISTDRQNGLSISTKNLVDENETYFIELKKALDSLKALNLK
                     LNTIIKNARAVIEKTGRSMENIRESSVGISKVVNYMKEIAYQTNLLALNASIEAARAG
                     ETGKGFTVVAEEVGKLAQRSAVHTKEITENIRKSLEDISEESKSVDSVTKAFDLILNS
                     YGEFERLVDNCYEYMQRFEGNKSGIIVSLNDLNQNASDIRITTNEQETAINETTAAIS
                     KISEQAFMLAQSVEELSEVVSFLGETEELINKLSTE"
     gene            948..3362
                     /locus_tag="PK683_01880"
     CDS             948..3362
                     /locus_tag="PK683_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNH07223.1"
                     /translation="MRTHGKIKQKLLLVNLSAALFILLLGGTVQFLILKHITENKIKQ
                     DLSELTEVSHNLVKTAVDSSVSNYLRALAEKNKSLLEYYYGLSRAGILSEPDAYLRAK
                     NEFLDPGYGKVGVTGYLAGVNSKGVIVIHPKSEGVDASKAPFMQKALKIEEGYLEYKW
                     KNTGETEERDKAAYLTYFKPWDLKVWVSSYKEEFYSLVDMQNLKTHLSTVTVGGKGHI
                     IIITYDGKTVFHPGQKNGAEELNLQGLTEQISQLSSSSEGSIEYRNKGDSADGKIHMA
                     FYKKMPEMKWIIVASAPVDDLFSTVYTMRNTILLNMIVITLVLGGALTWSSHYITNPI
                     SKLMNAIIRIGAGERNLEVRPESKDEIGELALRFNQMSSTIQQNMDKIEEQNRKILEQ
                     NEFLEERVLERTQELQNTLTLVQELKLQQDADYYLTTLLMHPLFRDRNRSCCVKTDFL
                     ISQKKKFEFRKFREELGGDLCVTGNLNFSGVKNTLFFNGDAMGKSMQGAGGAIVMGSI
                     LNSIMARSAAGKKVLTMSPEEWVHETFSEIQRVFEAFDGSMFVSCVLGLVSEDGSVLY
                     FNAEHPYTVLLRDRKAQFIEEEISVRKLGMPDQNELVLTRLKLEKGDMLIAGSDGRDD
                     LKIQNDDGESSIHHRPELFLNIVEEADGDLKEIQKKLTSIGELTDDLSLIKIFYEGKP
                     TGEKENFLSSSEITELIRNRKYTEAIYEIQKHPERNGFILTYYHGYCCMQKGQYSEAL
                     FFFEKAKEMNPDSFQIYFGLAKSYFQMEKFREAEQCAENVLRIKPDHLKAGRLSKMIR
                     EKILSE"
     gene            complement(3393..4055)
                     /locus_tag="PK683_01885"
     CDS             complement(3393..4055)
                     /locus_tag="PK683_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNH07224.1"
                     /translation="MLLKLYTSIFFILLMNCHVLNVQLEKRSYVSDKNQNYSDRYASY
                     GWGVFHSSSPRMIQCKEGLETVSIRRDTADAVIHYFLGGIISTRKIESRCPGDKENIA
                     ETLRKDKKISLNTIQFDPDSSRILDESHPIADELAKFMKEKKKVKIKITGHAAVTGQI
                     EKSRKLSKARAESVKAYLVSAGIAEDRIETEGLGESQPLVNGRDERANTLNRRVEFET
                     YE"
     gene            complement(4046..>4374)
                     /locus_tag="PK683_01890"
     CDS             complement(4046..>4374)
                     /locus_tag="PK683_01890"
                     /inference="COORDINATES: protein motif:HMM:NF018045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07225.1"
                     /translation="GDKMKYIIIMSLIYCQHARVELPPPASAACKKNDCAASVQEDSG
                     KTLSFQHKYYFGGLIPRKYEIKESEVCPGTGIRQIHQYYTFKDAALEQLTAFIYSPRT
                     VKIICY"
BASE COUNT         1349 a          838 c          989 g         1198 t
ORIGIN      
        1 agacagatca gcatatcgac tgacagacag aatggtctat ccatcagcac caaaaatctt
       61 gtagatgaaa atgaaacata tttcattgaa ctgaaaaaag cactcgattc actgaaggct
      121 ctcaacctga agctgaatac tatcattaaa aatgcaagag ccgtaataga aaagaccgga
      181 aggtcgatgg agaatataag agagtcttct gtcggcatat ccaaagtagt gaactatatg
      241 aaggagattg cctatcagac caatcttctg gccctgaatg catctataga agctgcgaga
      301 gcaggggaga ctggaaaggg atttactgta gtggcagagg aagtcggcaa acttgcacag
      361 agatctgcag tgcatacaaa ggagatcaca gaaaacatca gaaagtctct ggaggatatt
      421 tctgaagaaa gcaagtctgt ggattccgtt acaaaggcct ttgatctgat actgaacagt
      481 tacggtgaat ttgagaggct cgtggacaac tgttatgagt acatgcagag atttgaaggc
      541 aataaatccg gaatcattgt ttcactgaat gatctgaatc agaatgcatc ggatatcagg
      601 attactacga atgaacagga gactgccatc aatgaaacga ctgcggctat ttctaaaatt
      661 tccgagcagg catttatgct tgcacagtcc gtagaagaac tttccgaagt ggtttcattt
      721 ctcggtgaaa cagaggaact gatcaacaaa ctgagcaccg aatgactgaa tacagccgaa
      781 tatgataaat atcatttttc ggctgtgctg aaaatctgag cacagtgccc ctgcggaaga
      841 taaacagttt aaaaaagaat cactgacagt ctgatccaag aatataaatt tattgacaat
      901 atatgttttt aaatataacg tttgtacatc aggcaggtcg gcatacaatg agaactcatg
      961 gaaaaataaa acagaaactg cttttggtaa atctttctgc agctcttttc atcctgcttc
     1021 tgggaggaac tgtccagttt ctgattctga aacacattac cgaaaataaa attaagcagg
     1081 atttgagcga actgactgag gtttcgcata atctggtaaa aactgcggtg gacagttcgg
     1141 ttagcaatta tctgagagcc cttgcagaaa aaaacaaatc tcttctcgaa tactattacg
     1201 gtctgtcaag agcagggatt ctttctgagc cggacgctta tttacgcgcc aaaaatgaat
     1261 ttctggatcc gggttacgga aaagtgggag tcaccggcta tctcgcaggc gtcaattcaa
     1321 agggtgtcat agtcattcat cctaaatccg agggagtgga tgcttccaag gctccgttta
     1381 tgcagaaggc cttgaaaata gaagagggat atctcgaata caaatggaag aatacaggag
     1441 aaactgaaga gcgggacaaa gccgcctatc tgacatactt caaaccctgg gacctgaagg
     1501 tctgggtatc ttcctataag gaagagtttt attcccttgt ggatatgcag aatctgaaga
     1561 ctcatctgag cacagtgact gtgggaggga aaggacatat tatcattata acatatgacg
     1621 gaaagacggt gtttcatccc ggtcagaaaa acggagcaga agaactgaat cttcagggac
     1681 tgactgaaca gatttctcag ttgagcagtt cttcagaggg cagcattgaa tacagaaaca
     1741 aaggagattc tgctgacgga aaaattcata tggcctttta taaaaaaatg cctgaaatga
     1801 aatggatcat tgtagcgtct gctcctgtgg atgatctgtt ttcaacagta tatacaatga
     1861 gaaatacaat tcttcttaat atgattgtca ttacactggt tctcggaggc gctcttacct
     1921 ggagcagtca ttatataaca aatcctattt caaaactgat gaatgcaatc atcaggattg
     1981 gagccggaga aagaaatctg gaagtccgtc cagaatcaaa ggatgaaata ggagagctgg
     2041 ctctgagatt caaccagatg agttccacca ttcagcagaa tatggacaag attgaagagc
     2101 agaacagaaa gattctggag cagaatgaat ttctggaaga gagggttctt gagagaaccc
     2161 aggagcttca gaacactctg actctggttc aggagctcaa actgcagcag gatgcagact
     2221 attatctgac tactctcctg atgcatcctc ttttcaggga caggaacaga agctgctgtg
     2281 tcaaaacgga ttttctgatt tctcagaaga aaaaatttga atttagaaaa ttcagagagg
     2341 aactgggagg agatctctgt gtgacaggaa atctaaactt ctctggtgtg aaaaatacat
     2401 tattttttaa tggtgatgca atgggcaagt ccatgcaggg tgcaggggga gcaattgtga
     2461 tgggttccat cctgaattcc attatggcca gatccgctgc aggaaaaaaa gttctcacga
     2521 tgagtcctga agaatgggtc cacgaaacat tcagcgagat acagagagta tttgaagcat
     2581 ttgacgggtc gatgttcgtg tcatgtgtgc tcggccttgt gtctgaggac ggttctgtcc
     2641 tgtattttaa tgcagagcac ccctatacgg tgcttctgag agacagaaag gctcagttca
     2701 tagaagagga aatcagtgta agaaaattgg ggatgcctga tcagaatgaa ctggtgctta
     2761 ccagactcaa gcttgagaaa ggtgatatgc ttattgcagg aagcgacggc cgggacgacc
     2821 tgaaaattca gaatgatgac ggtgaaagtt caattcatca caggccggaa ctttttctga
     2881 acattgtaga agaggctgac ggtgatctca aagaaattca gaaaaaactg acttccatag
     2941 gtgaacttac ggatgatctc tcactcatta aaatatttta tgaggggaaa ccgacaggcg
     3001 agaaagagaa ttttctttct tcttcagaaa ttaccgaact gatcagaaac agaaaatata
     3061 ctgaagccat atatgaaata cagaaacacc ctgaaagaaa cgggtttatt ctgacctatt
     3121 atcatggata ctgctgtatg cagaaaggtc agtattcaga ggctcttttc ttttttgaaa
     3181 aagcaaaaga gatgaaccct gacagtttcc agatatattt cggacttgcc aaaagttatt
     3241 tccagatgga aaaattcagg gaggctgaac agtgtgcaga aaatgtgctt cgaatcaaac
     3301 ctgatcattt aaaggcagga aggctcagta aaatgatcag ggaaaagatt ctttctgaat
     3361 agacccagtg atctcctgaa actgcagaaa tgtcattcat aagtttcaaa ttcgactctt
     3421 ctgttcaggg tatttgcgcg ttcatctctg ccgttcacca gaggctgtga ttctccaagt
     3481 ccttctgtct caatccggtc ttcagctatg cctgcagaaa ccagataggc cttgactgat
     3541 tcagctctgg ctttggaaag ctttctgctc ttttcaatct gtcctgtaac ggctgcgtgt
     3601 cctgtaatct tgattttgac ttttttcttt tctttcatga attttgccag ttcatcagca
     3661 atcggatgag attcatccag tatcctgctt gaatcaggat cgaactgaat tgtgttcagt
     3721 gaaatctttt tatcctttct taatgtttct gcaatatttt ccttgtcacc tggacatctg
     3781 ctctcaattt ttctcgtgct gatgattccg cccaggaaat agtgaatgac tgcatccgct
     3841 gtatctcttc tgatgctgac cgtttccagg ccctctttgc actgaatcat tctgggagaa
     3901 gacgaatgaa aaactcccca tccgtacgat gcatatctgt ccgaataatt ctgattttta
     3961 tcagatacat aggatctttt ttccagctgc acattcagaa catggcagtt cataagaaga
     4021 atgaaaaata tacttgtata cagtttcagt agcatatgat tttgactgtc ctcggagagt
     4081 agataaatgc tgtcagctgt tcaagggcag cgtctttgaa agtatagtac tgatgaatct
     4141 gccgaatccc tgtgccggga cagacttctg attcctttat ctcgtatttt ctggggatca
     4201 gtcctccaaa ataatactta tgctggaagg acagagtttt tccgctgtcc tcctgcactg
     4261 aagcagcgca gtcatttttt ttgcatgcgg ctgatgcagg aggcgggagc tccactcttg
     4321 catgctgaca gtatattaat gacattataa tgatatattt cattttatca cctc
//
LOCUS       DAOQGJ010000073         4373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10746_length_4373_cov_3.325151, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000073 DAOQGJ010000000
VERSION     DAOQGJ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10746_length_4373_cov_3.325151"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /locus_tag="PK683_01895"
     CDS             complement(<1..1546)
                     /locus_tag="PK683_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH07226.1"
                     /translation="MKSWILFVLLSVFFNCLQTKKSGFDLKQGGLPGIFLFGVSSGLI
                     DLCPTCDRTAPTVTVTNLKTKGTVETGILMGTSADNVAVSKVEIQLNSGSYQTATGTT
                     SWTYKLPTTGWTGGNTNTINIKVTDTSGNVGTASLSTLTKGLNSDVNGDGYNDIAVGA
                     YGYNSNQGRVYVFSGSANGVTDSSAANAGKIFNGYASAAFGKNVLMADFNNDGYGDLL
                     VCSSVYTSSNNRGDIYYGSATGLSSTPSLTFSPPSGSATQFCYTIAAADFNSDGYLDL
                     AISDGAVNPGIVYVYYGSSNGLSTTQSISIVDGSTTNEGVSMSAGDINGDGFPDLVMS
                     NSIDSTVYIRFGSVNGLGAASTSFTGLLASQNVSLADLNGDGKQDLILGDPSATSGAS
                     QGKVAVYLANGTTFNSTASVTLDGDSSQYRFGSFLLGRDINGDGINDLIISSDRADLN
                     SLADSGAVYIFNGCASTVICNGSGGATAKSNLRIAGDSASLNLGSSVKVKDLNNDGFP
                     ELIITAV"
     gene            complement(1657..3855)
                     /locus_tag="PK683_01900"
     CDS             complement(1657..3855)
                     /locus_tag="PK683_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07227.1"
                     /translation="MKKPTDGTNQIFSIIKSEKIRAKTALYFILTALLILSFCAGSRT
                     QPRLVKGILDLRNESAWDLKTDGIVQLRGEWEFYWKQFLEPSSFPNQITPIYFDSPSV
                     WNGNTVSGSKIGGYGYATYRARILLPSSEHRKLALKIQDQAHSWKLYINDKFIKEWGK
                     TGTSPETSVPVLQTDTVVFESDSESVDIIFHVSNFHHRVGGLRYEILFGPEDRIFYEK
                     QRVLIMESYLFGGFLMIAVYHFAIFYFRRRNLAPLFFGLFCLSVMMYTSSTGERVVYN
                     VFPDLFSWENSYRLEFTWISCMSSFFILFFISLYPQKQKSFLSVISWISIAVNTCYTL
                     MLPFTEPKFFTEFLLVLDLSRVLIGSTIIYYLIKYALQGKEGSLILLSGFFILFLCSI
                     NDTLAARSILHTPRMTKGGLAVMLFMQAIMLAKIFTKDYKMAISLSESLSLTNQAYSR
                     FVPSEFLNLLDKKSILDIELGDQVQKEMTVLFSDIRSFTSLSERLSPKENFQFLNAYL
                     KRIVPHIKNHNGFIDKYIGDAMMGLFPGNADDALQASISIQKEIADYNRHREQSGFSK
                     IKVGIGLHTGNLILGTIGHRERMEGTVISDAVNLASRIEGLTKHYGSGILISENTFDA
                     LEEPLNYQFRLLDNVRVKGKTDSIFVIEILDGFPPQQIEKFLDTKKEFETANLLYKQK
                     AFGASIELFRKVLSENPDDSAAEFYLKRAEYYQKYGTPPEWENGEVFAEK"
     gene            4115..>4373
                     /locus_tag="PK683_01905"
     CDS             4115..>4373
                     /locus_tag="PK683_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07228.1"
                     /translation="MSVRMGISRLTYSSYANTICCSDAVIFRKHQENSAVSALAAASI
                     LAAAPILAAAPILAAAPILAAASMLAAASMLAAASMLAAASM"
BASE COUNT         1249 a          966 c          879 g         1279 t
ORIGIN      
        1 ctactgcagt gattatcagt tctggaaatc catcattgtt tagatctttt acttttacag
       61 atgatcctaa atttaagctg gctgaatctc cggcaattcg caaattactc tttgctgtgg
      121 ctccgccgct accattgcaa attaccgtac tggcacagcc attgaaaatg tatactgcac
      181 cactgtcagc aagactgttt aaatcagctc tgtcgctgct tataatcaaa tcattgattc
      241 catctccatt tatatctctt cctaaaagga aactgccaaa tctgtattgg ctgctgtctc
      301 catctaaagt taccgaagca gtgctgttga aggttgtacc gttggccaaa taaacagcca
      361 cctttccttg acttgccccg gaggttgcag atggatcgcc tagaattaaa tcctgtttcc
      421 catcaccgtt taaatcagct aggctcacat tttgacttgc caaaagaccc gtgaatgacg
      481 tactcgctgc ccctaaacca ttcacggaac caaatcgaat gtatacagtt gaatcaatgc
      541 tgtttgacat cactaaatct ggaaacccat ctccgttaat atcacctgca gacatactga
      601 ctccttcatt tgttgtcgat ccatccacta tagaaatact ttgtgttgta gagaggccat
      661 ttgatgatcc gtaataaaca tagacaattc cgggatttac agcaccatca ctgattgcca
      721 gatccaaata tccgtcactg ttgaaatcag ccgctgcaat agtgtaacaa aattgagtag
      781 cagaaccgct gggaggagaa aaggtaagtg acggtgtgct ggataagcca gttgccgacc
      841 cataatatat gtcacctcga ttattgctgc tggtgtaaac actagagcat accagtaaat
      901 ctccataccc atcattatta aaatcagcca tcaatacatt cttcccgaag gcagcagatg
      961 cataaccatt gaaaatcttt ccagcatttg cagcagaact gtcagttaca ccatttgctg
     1021 agccactgaa aacgtaaact cttccctgat tactattata gccatacgcc ccgacagcaa
     1081 tatcattgta accatctcca tttacatcag agttcagtcc ttttgtaagt gtgcttaggg
     1141 atgctgtgcc gacgttcccg ctggtgtctg tgaccttgat attgatggta tttgtatttc
     1201 cgcctgtcca gccggttgtg ggaagtttgt acgtccatga cgttgtacct gtcgctgtct
     1261 gataagaacc ggagttgagc tggatttcca ccttggagac tgcgacattg tctgcggatg
     1321 ttcccatcag tattcctgtc tccactgtac cctttgtttt gaggttggtg actgtgactg
     1381 tgggggcagt tcgatcacaa gtaggacata aatcaattaa tccagaggat actccaaata
     1441 gaaagattcc tggaagtccg ccctgcttga ggtcaaagcc ggattttttg gtttgaagac
     1501 agttgaaaaa cacgcttaaa agaacgaata gaatccaaga tttcatacta taagtaacac
     1561 aaacagaata cggctttttc agaaaacaga aaaattttga cttttgcacc gcttcagtct
     1621 gcagaaaaac tttcagaagg caggatatga aaaaatttat ttctctgcaa atacctcacc
     1681 gttttcccat tcaggaggtg ttccatattt ttgatagtat tccgcccttt tcagataaaa
     1741 ttctgcagca gaatcgtccg gattttcaga gaggactttt cggaacagtt caatgcttgc
     1801 tccgaatgct ttctgtttgt acagaagatt tgctgtttca aattcttttt ttgtatccaa
     1861 gaatttttca atctgctgcg gaggaaatcc gtccagaatc tcaatcacaa aaatgctgtc
     1921 tgtctttcct ttgactctta cattgtccag aagacggaac tggtaattca aaggttcttc
     1981 gagagcatca aatgtatttt cactgatgag gataccgctc ccgtagtgtt ttgtcagacc
     2041 ttcaatcctg gaagccagat tgacggcatc ggaaatcaca gtcccttcca tcctttccct
     2101 gtgtccgatt gtgcccagaa tcagatttcc tgtgtgcagt cctattccca ctttgatttt
     2161 ggaaaaaccg gactgttccc tgtgtctgtt gtaatctgca atttccttct ggatggaaat
     2221 ggatgcctgg agcgcatcgt ctgcatttcc ggggaataac cccatcattg catctccgat
     2281 gtatttgtct atgaaaccgt tatggttttt gatatgaggt acaattcttt ttaagtacgc
     2341 attcaggaac tggaaatttt ccttaggtga aagtctttcg gaaagcgaag taaaagaacg
     2401 tatgtcgctg aaaaggacgg tcatttcctt ttgtacctga tctccgagct caatgtccag
     2461 aatggatttt ttatccagaa gattcaggaa ttcagaagga acaaacctgc tgtacgcctg
     2521 gtttgtgaga gacagagact ctgaaagact gattgccatc ttatagtctt tagtgaatat
     2581 ttttgcaagc atgatcgcct gcatgaacag cattactgca agtcctccct tagtcatccg
     2641 aggagtatga agtatggatc tggctgcgag ggtatcattg atggaacaca aaaacagaat
     2701 aaaaaaaccg gagagcagaa tcagtgaacc ctccttcccc tgaagggcgt atttaataag
     2761 ataatatatg attgtcgatc cgataagaac cctggataag tccagcacaa gaaggaactc
     2821 tgtaaaaaac ttaggttcag tgaaaggaag catcagtgta taacaggtat tcacagcaat
     2881 cgaaatccat gatatgacag aaagaaaaga cttctgtttc tgaggataca atgatataaa
     2941 aaaaagtatg aaaaaactgg acatacagga aatccaagta aactcaagac ggtaactgtt
     3001 ttcccagctg aaaagatctg gaaatacatt gtataccact ctttcccctg tggaactggt
     3061 atacatcatt acagacagac agaacagtcc gaaaaacagc ggagccaggt ttcttcttct
     3121 gaaatagaaa attgcaaaat ggtatactgc tatcatcagg aatccgccga aaaggtagga
     3181 ctccataatc agcactctct gtttttcata aaaaatcctg tcttccggac cgaacaggat
     3241 ctcgtatctg aggccgccta ctctgtgatg aaaattggaa acgtgaaaaa taatatccac
     3301 tgattcagaa tctgattcaa atacaacagt gtctgtctga agcacaggaa cggatgtttc
     3361 cgggctggtc ccggtttttc cccattcctt aatgaattta tcattaatat aaagtttcca
     3421 gctgtgtgcc tgatcctgaa ttttcagtgc aagttttctg tgctcggaac tcgggaggag
     3481 aatccttgcc ctgtacgttg cataaccgta acccccgatt ttacttccgc tgacagtatt
     3541 tccgttccag acggaggggg aatcaaaata gatcggagtg atctgatttg gaaaactgga
     3601 cggttccaaa aactgcttcc agtaaaattc ccattctcct ctcagctgaa caatcccgtc
     3661 cgttttcaga tcccaggcgg attcatttct caaatccaga attcctttta caagcctagg
     3721 ctgggttctg cttccggcac agaaggaaag gatcagaagc gcagtcagta taaaatacag
     3781 tgctgtcttt gcccttattt tttccgattt tataatcgaa aaaatttgat tcgttccgtc
     3841 tgtcggtttt ttcataatag gattggaaat cagacaggtt attttgtaaa taagtttctt
     3901 attgaaatcg tggcaatttt ttccgtatat tcacagtacg gaaaggcagg ttcttcagga
     3961 acactgaacg gactctgtca aatttcctta cattaaaaaa agcagaaatg agactcttga
     4021 tgcagaatca tcctttcgaa agacctgatg gatctcacca aatgcagaaa tgataatata
     4081 ttttaatatc tgtaattcta tttaaatatg caatatgagt gttcggatgg gaatcagcag
     4141 actgacttac tcatcttatg cgaatacgat atgctgttca gatgcagtaa ttttcaggaa
     4201 gcatcaggaa aattcagcag tttccgcgct tgcagcagct tccatacttg cggcagctcc
     4261 catacttgcg gcagctccca tacttgcggc agctcccata cttgcggcag cttccatgct
     4321 tgcggcagct tccatgcttg cggcagcttc catgcttgcg gcagcttcca tgc
//
LOCUS       DAOQGJ010000074         4366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10777_length_4366_cov_3.516354, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000074 DAOQGJ010000000
VERSION     DAOQGJ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10777_length_4366_cov_3.516354"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK683_01910"
     CDS             complement(<1..231)
                     /locus_tag="PK683_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07229.1"
                     /translation="MRVILVIVLSFLSSISVHSEEKEVRIISLTEDCSSGKCVPNPWW
                     VRNSALENYSIDSFHPDENWKKADKFPLWMNKF"
     gene            complement(347..2926)
                     /locus_tag="PK683_01915"
     CDS             complement(347..2926)
                     /locus_tag="PK683_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07230.1"
                     /translation="MDFLFWPGLLVLFCTSVFSEEVTVGIQAVSGERIEILNTDSNST
                     WWISDRDHDPKVFSEELLSDPGRLSRISESGFIPAPVPASVSNVKNFPKDTMSAWYAK
                     ILFLKKDSPGHLALELGLIDDSDITYFNGVKIGETGNLSSKSAIGYDRQRIYEIPDSL
                     LRKNGANIILIQVRGYFPGEWGMSRGRTAVGPAKLLYSEYYKTNYVELLFLTVYFTMG
                     TYFIFLFMRRRAELENLLFGLFSYDLVIYSFLKTQFRFEFGWDFLTAKRIEYFCLYTA
                     VPAFYYFLRLSFPFSRSKRMKIWDWLTAIICILILGCILIVMMTGEVTLWWNIQKYFV
                     QPLWLPLLLGSAGLLVLQRIRGSRDAGYMLIGFSFPLAGVTADILSSRGVIVFPPVLA
                     YSFLFFVLSIGLVLANRFVRLNEEIEDLNQNLEQKIENRTVELQESLNQVRTLKEQQD
                     GDYFLTSLLLDPLSGNHSKSENFSISIHVRQKKVFSFKKWNASIGGDICIVHSVRLKN
                     KPFIIFLNGDAMGKSIQGAGGALVLGTVFNAVVKRTRMNSGAADRYPEHWLKECFHEL
                     QSVFISFNGTMLASAFIGLADEENGALYYLNAEHPEPVLFRDGKANFLDAEKPLHKLG
                     VDLEHGPLRIRLFQMEPYDTLIVGSDGRDDLDIGMNESGQRVINEDETLFLQCVEKGN
                     GQLADIEKELLVCGKLTDDFSLIRVGFSRHNSGSRISGGHHYSAESKSVRKRIQQNLI
                     AKKYQEAAELIDGHIAEYPLDTVCLYWGMLAYKGIWRQTGAKDTLRLAIDYGERCRVR
                     TPENLNNLISLSDAYLLSGNSEKALHTAENAKLTAPDDSRVSQLFERLNPQNGSIENT
                     GEL"
     gene            3247..>4366
                     /locus_tag="PK683_01920"
     CDS             3247..>4366
                     /locus_tag="PK683_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH07231.1"
                     /translation="MNLKMLSLIMIFSVLLFSSCGHSENGAENQRFHHGHRQLGSHSS
                     YLFTDREESLEEILKPEKTVQFQKSRTDYPNFGIQNRELWLKVELNLNDNTESVIEAA
                     SPLLSKIRFFQYCDGKQISFYQSGMDIPNEKLPLHPNYQFPVKPWNGPCQFFIAVQSN
                     DSMTVPLFYWEKSALQKMDTVRHLLFGLFFGLMISLALYNFLLFLSIRNRAYLWYVFY
                     ILTFTVFYLSVYGYFRFLFGSLCHNGISYVVMITSILTAAFALLFGNQFLQIRQIKPR
                     LTEFIHYIVMFGLLLLIPIRFVTINQGIFIGNLFPGFAISVLVYAIYVSIRSGYKPAF
                     YFAGAWGTLLVSVSMFIAENLGLILANAVTHYSQIIGAA"
BASE COUNT         1215 a          962 c          883 g         1306 t
ORIGIN      
        1 gaatttgttc atccagagag gaaatttgtc agcctttttc cagttttcat cgggatgaaa
       61 cgaatcaatt gaatagtttt ccagggcgga gtttctgacc caccagggat ttggaacgca
      121 tttgcctgaa ctgcagtcct cggttaagct gatgattctg acttcttttt cttcagagtg
      181 gacggaaatt gaacttagaa aagataaaac aatgactaaa ataactctca tgacagcctc
      241 ccgattgcag gcacacgctg tattagattt tttgaatatt actgagcaca agcataatca
      301 agtctcagaa taagatagtg aagtcaatgc ccggacaatc ctgagtttat aattctcctg
      361 tattttcgat ggaaccgttc tgaggattca gtctttcaaa cagctgactg actcttgaat
      421 cgtccggtgc agtcaatttt gcattttcgg cagtatgcag ggctttttct gaatttccag
      481 agagcagata tgcatcagaa aggctgatca gattattcag attttcaggg gttctgactc
      541 tgcatctttc cccgtaatca attgcaagcc gcaatgtatc ctttgcgccg gtctgcctcc
      601 aaatgccttt ataggccagc attccccagt aaagacagac tgtatcgaga gggtattctg
      661 caatatgtcc gtcaatcagt tcggcagcct cctgatattt tttcgcaatt aaattctgct
      721 gaatcctttt tcttactgat tttgattcag cggaataatg atgcccgccg gaaatcctgg
      781 acccagaatt atgcctgctg aatccgactc tgatcagaga aaaatcgtcg gtcagttttc
      841 cgcagacaag cagttccttt tcgatatcag caagctgtcc gttgcctttt tctacgcact
      901 gcaggaataa tgtttcatct tcattgatga ctctctgtcc gctttcattc attcctatgt
      961 ccagatcatc acgtccatcc gatcctacaa taagtgtgtc atacggttcc atctggaaaa
     1021 gccttattcg aagcggaccg tgttccagat ctacacccag cttatgaagg ggtttttctg
     1081 catccagaaa atttgccttt ccgtcacgga acagtaccgg ttcaggatgc tctgcgttca
     1141 gataatatag tgctccgttt tcctcatccg ctaatcctat aaatgcggat gcgagcatag
     1201 taccgttaaa ggatatgaat acactctgca gttcatggaa acattctttc agccaatgtt
     1261 ccggataacg gtcagccgct ccggaattca ttcttgtgcg tttcacgact gcgttgaata
     1321 cagttccaag cacaagtgct cctccggcac cctgtatgga cttgcccatc gcatcaccgt
     1381 tcaggaaaat tatgaaaggc ttatttttca gtctgactga atgaacaatg cagatatctc
     1441 ctccgatgga tgcattccat tttttaaagg agaacacctt cttctgtctg acatgaatgc
     1501 ttatgctgaa attttccgat ttggaatgat ttcctgaaag cggatccaga aggagtgatg
     1561 tgagaaaata atctccgtcc tgctgttctt tcagggtacg cacctgattt aatgattcct
     1621 gcagctcaac ggttctgttc tctattttct gttccagatt ctggtttaaa tcttctattt
     1681 cttcattaag acgtacaaaa cggttggcaa gcacaagtcc tatgctcaga acaaaaaaca
     1741 gaaatgaata ggcgagcaca ggaggaaata caatcacccc tctgctggaa agaatatctg
     1801 ccgttacgcc agccagagga aacgaaaaac cgatgagcat ataaccggca tccctgctgc
     1861 ctcttattct ttgaaggaca agaagaccgg cagagcccag aagcagagga agccagagag
     1921 gctgaacaaa gtatttctgt atattccacc agagtgtcac ttctcccgtc atcattacaa
     1981 tgaggatgca tcccaaaata agtatacata tgattgcagt aagccagtcc cagattttca
     2041 tccgttttga tctggaaaac gggaaagaca gacgcaggaa gtaatagaat gccggaacgg
     2101 ccgtgtaaag acagaaatat tcgatcctct ttgccgtcag aaaatcccat ccgaattcaa
     2161 aacggaactg cgtcttcaag aatgaatata tgaccagatc gtatgaaaac agtccaaaga
     2221 gaagattttc gagctccgcg cgtcttcgca taaagagaaa tataaaatag gtccccatcg
     2281 taaagtaaac tgtcaaaaag agaagctcaa cgtaattcgt tttataatat tcactgtata
     2341 gcaatttggc aggtccgaca gctgttcttc ctctggacat accccattct cccggaaaat
     2401 atcctctgac ctgaatcagt ataatatttg ctccgttctt tctcagaaga gaatctggaa
     2461 tttcataaat cctctgcctg tcgtaaccga ttgcagattt ggaagaaaga tttccagttt
     2521 caccgatctt cactccgttg aagtaagtga tgtcagagtc gtcaatcagt cccagttcca
     2581 gtgcaagatg tccggggcta tcctttttta aaaaaagaat ctttgcatac caggctgaca
     2641 ttgtatcttt tggaaagttt tttacattgc tgactgatgc aggaacagga gcaggaataa
     2701 agcctgactc agaaatacga ctgagtcttc cgggatctga cagaagctct tctgagaata
     2761 ctttgggatc atggtctcga tccgaaatcc accatgttga attgctgtct gtattcagga
     2821 tttcgatcct ttccccggac acagcctgaa tgccgacagt cacttcctcc gaaaaaacag
     2881 aagtgcagaa tagaaccaga agtcccggcc aaaaaaggaa atccatcagt cttttagata
     2941 atatatacag gttaaaataa ttcagcattc tcagtattcc tatttttaaa ggctaatttt
     3001 cagagatgca agtgattaaa gcaatccttt ttttggtttc gattgagata aatttgacgc
     3061 atctgcgcag aagaatggct ttttattcag aatgaaccgt gaggaaaact attcctcgaa
     3121 aaacagtgaa aaggcatatc cgaataatat gtttgcctga tttttgctct tgggcatatt
     3181 tgatgaatca gaggatgttc tgtatttggg aaaacaggat agacgacttt acaagggaac
     3241 agatatatga atctaaaaat gctttctctt ataatgatct tctctgtcct tttattctca
     3301 tcctgcggac attccgaaaa cggcgcggag aaccaaaggt ttcatcacgg tcacagacag
     3361 cttggatctc attcctcgta tctgtttact gacagggaag agtcacttga agaaattctc
     3421 aaaccggaaa aaacggttca gtttcaaaaa agcagaactg actatcccaa ctttggaatt
     3481 cagaatagag aactctggct gaaagtggaa ctgaatctga atgacaatac agaatctgta
     3541 attgaagcgg catctcctct tctttccaag atccgttttt ttcagtactg cgacggaaag
     3601 cagatttcgt tttatcagtc cggaatggac atcccgaatg aaaaactccc cctccatccc
     3661 aactatcagt tccctgtaaa accctggaac ggaccctgtc agtttttcat cgctgtacag
     3721 tcaaatgatt caatgactgt tcctctgttt tactgggaaa aatccgcgct gcagaaaatg
     3781 gatacagtca gacatcttct cttcggtctt tttttcgggc tgatgatttc tcttgcattg
     3841 tataattttt tacttttcct ttcgattcga aaccgggcgt atctctggta tgttttttat
     3901 atactgactt ttactgtatt ttatctgagt gtatacggtt atttccgttt tctgttcgga
     3961 tctttatgcc ataacggaat ctcatacgtt gtgatgatta caagcattct gacagcagca
     4021 tttgcacttc tgttcggaaa tcagtttctg cagatccggc agattaaacc caggctgact
     4081 gagtttattc attatattgt aatgttcggt ctgctccttt tgattcctat aaggttcgtt
     4141 acaatcaatc agggtatttt tatcggcaac cttttccctg ggtttgcgat cagcgttctg
     4201 gtttatgcaa tttatgtcag tatacgttcc ggatacaagc cggcgtttta ttttgcggga
     4261 gcctggggaa ctcttctggt gtccgtgagt atgttcattg cagaaaacct cggtctgatc
     4321 cttgcaaatg cagtcactca ttacagccag atcatcggag ctgcac
//
LOCUS       DAOQGJ010000075         4340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10874_length_4340_cov_3.510618, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000075 DAOQGJ010000000
VERSION     DAOQGJ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10874_length_4340_cov_3.510618"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PK683_01925"
     CDS             <1..475
                     /locus_tag="PK683_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNH07232.1"
                     /translation="GFGYTEILDPVSQTSLPVISTLKNMIAIMLFLITGAHRKVIESI
                     AFSFEKASIYHLTAEMENGIFRTFEYSIGAMFLIAFKIALPILGVIILVTAAEALMGK
                     AAPQMNILQLSFPAKIAIGLIVMILVTPFIEQQMVQGFELSLDRIDKMLRGWPRP"
     gene            475..1605
                     /locus_tag="PK683_01930"
     CDS             475..1605
                     /locus_tag="PK683_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNH07233.1"
                     /translation="MTPTDVSDWQSLALADFRIDLQMFAAEDEGRTEEGSERRRREER
                     DKGNVPRSQELPSALVLLTSLASIYMLGGFIFEKCFLLFRRYFENIPQYSRFGNEEMR
                     RLFLSAASDLMTIFIPVVGMGFLVAIAGNIVQVGFLFSPGAASFNFSRIAPNFKRVLP
                     TGQTFFNLAKALAKVILIGWISYIIISMDFYSLLMSGNMGVKAALALVMRSAFKIMLT
                     AGIVFFVISIFDFYYQRFQFEESIKMTPSEAKQELKESEGDRSILNRRRQMVREFIRK
                     GMIQRVPKADVIIVNPTHYSVALIYDPAIHFAPVVTAKGADELALLIRRLARKHNIPI
                     VEDRVQARLLYDEVEVDEPIPAKFFRAISLIIARFDKYRRVV"
     gene            1605..3728
                     /gene="flhA"
                     /locus_tag="PK683_01935"
     CDS             1605..3728
                     /gene="flhA"
                     /locus_tag="PK683_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNH07234.1"
                     /translation="MQEKGPWYAQSEVVLGIGVIAILAMLIIPLPGFILDLLIVVSMA
                     MGMIILLTSLSMKEPSDFSIFPNLLLITTLYRLALNVSTTRQILSQGSAFNSHIIDAF
                     GTFVVGGGSGLGKYMVGFIIFLILIIVQILVITKGATRIAEVAARFTLDALPGKQMAI
                     DTELSSGNINEEEARTRRKKVQKEVDFYGAMDGASKFVQGDVRAGLIITAINLIGGII
                     IGMAIRKESFGFAVETYGKLTIGDGLVSQIPGLLATTATGMIVTRAASEKELSQEFKS
                     QIFSNPKTLYVVSACLALAAFIPGLPMFSLFFFSIGLAYLAYIIDRNAKETITSIEKK
                     EAENKAEKKPDYYKELRTDPIEVELGLNLVPLVNASLGGVLLDQITNLRRRFATDTGL
                     VIPAVRILDNLELDPDSYAIKIHGVIVGDSKIRPTKLMAIDNSGKRALDQIMGEEFLE
                     PTFGFKAKWIDPADKVDAENKGAAVVDPSTVIVTHLKELISNHAASLLGREEVKNLLD
                     HVKTSYPTLVSEIDEKKLGVGLIQQVLQNLLREGLGIKNLASILEAILNNEPKTRDPF
                     FLSESVRQAISRQIVTDYLSKDGKLHVVTMDPKVSEKLSKSIVVDPIEGSLISVPPDY
                     YHKLIESIAREYNRFHSEGRFPIIVTSRALRLPLSYMLAKEFPPRNFAVLAMEEIHAA
                     VKTSMDGIITVQTADPVRARGSNYE"
     gene            3740..>4340
                     /locus_tag="PK683_01940"
     CDS             3740..>4340
                     /locus_tag="PK683_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Positive regulator of class III flagellar genes;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNH07235.1"
                     /translation="MDFVKIRGKDIQDCFMQMKMKYGPEAHVYEQRIVTEGGIFGTGF
                     LAKKYVEIEIGVPEQQSSKEKVEKKLQDLKELLKQKSEEEGPKKKTLTNMKPLLQREK
                     EKENSKQTVKDDYISDDFLDNDDEEEDYIPPSNLGLSIKDELTKKESVSASAEKEKSS
                     QIYESVYLKRMKDKLVRDGFSNDYAEEVISKADKYLSYVD"
BASE COUNT         1268 a          889 c         1036 g         1147 t
ORIGIN      
        1 gggattcggg tatacggaaa tcctcgatcc agtgagccag acatctcttc cagtcatcag
       61 cacactgaaa aatatgattg ccattatgct ctttctcatc acaggagcac acagaaaggt
      121 gatcgaaagc attgcatttt cctttgaaaa ggcaagcatc taccatctga ctgcagaaat
      181 ggaaaacggg atattccgta cttttgaata ttccatcggt gcgatgtttc tgattgcatt
      241 caagattgcg cttccgattc tcggtgtcat tatactcgtc acagctgccg aggcactgat
      301 gggaaaggct gctccccaga tgaacattct tcagctcagt tttcccgcca aaattgccat
      361 cggactgatt gtaatgattc ttgtcacacc tttcattgag cagcagatgg tgcagggatt
      421 tgaactgtct ttggacagga tagacaaaat gctccgaggc tggccccgcc cgtgatgact
      481 ccgactgacg tttcagactg gcaaagtctt gcactggccg actttcgaat tgatctgcag
      541 atgtttgctg cagaggatga aggccgtact gaagagggga gtgagagacg aagaagggaa
      601 gaaagagaca agggaaatgt ccctaggtct caggaacttc cttcggcact tgtgcttctg
      661 acttctctcg catccattta tatgctggga ggattcatat ttgaaaaatg ttttcttctg
      721 ttcagaagat actttgaaaa tatccctcag tactcaagat ttggaaatga ggaaatgaga
      781 agactttttc tgagcgcggc ctcggatctg atgaccattt ttattcctgt cgtcgggatg
      841 ggttttttgg tagcgattgc aggaaatatc gtccaggtag ggttcctgtt ttcgcctgga
      901 gctgcttctt ttaatttcag cagaattgcc ccgaatttca aaagagtact tcctacgggg
      961 cagactttct tcaatctggc aaaagccctc gccaaggtga ttctgatcgg ctggatcagt
     1021 tatatcatca tatccatgga tttttacagt cttctgatga gcggaaatat gggagtcaag
     1081 gctgcccttg cacttgtaat gcggtccgca tttaaaatta tgctgacggc gggaatcgta
     1141 ttttttgtca taagtatttt tgatttttac tatcagagat ttcagtttga agagtccatc
     1201 aaaatgactc cttccgaggc aaagcaggaa ctgaaagaaa gtgaggggga tcggagcatt
     1261 ctgaaccgaa gaaggcagat ggtcagagaa ttcatccgga agggaatgat ccagagagtt
     1321 ccaaaggcag atgtcatcat tgtaaatcct acacactatt ctgtcgcact catttacgac
     1381 ccggccattc attttgcacc ggtggtcact gcaaaaggcg cggatgaact cgccttatta
     1441 attagaagac ttgccagaaa acacaatatt cccattgtgg aagatagagt gcaggcgaga
     1501 cttctttatg acgaagtaga ggtcgatgaa ccgatccctg caaaattttt cagagcgatt
     1561 tcactcataa ttgcccgatt tgataaatat aggagagttg tataatgcag gaaaaggggc
     1621 cttggtacgc acagtcagaa gttgtattgg gcatcggcgt catagccatc cttgcaatgc
     1681 tgattatccc tcttcccgga ttcatcctgg atcttctgat tgtagtcagt atggcaatgg
     1741 ggatgatcat acttctgact tctctttcca tgaaagagcc ttctgatttt tccatattcc
     1801 cgaatctgct tctcatcacg actctgtaca gacttgcatt gaacgtatcc acaacccgac
     1861 agattctgtc tcagggaagt gcattcaaca gtcatatcat tgatgcgttc ggtacattcg
     1921 ttgtaggggg cggttcaggt ctcgggaaat atatggtcgg attcattatc tttctgattc
     1981 tcatcattgt tcagatctta gtcatcacaa agggtgccac caggattgcg gaagtggccg
     2041 ccagatttac tctcgatgcg cttccaggaa aacagatggc aatcgacact gagctttcga
     2101 gcggaaacat caatgaggaa gaagccagaa cacgaagaaa aaaagtgcag aaagaagtgg
     2161 atttctacgg ggctatggac ggtgcgagta aattcgtgca gggagacgta cgggcgggac
     2221 ttatcattac tgcaatcaat ctgatcggag gaatcatcat agggatggcc attcgtaagg
     2281 aaagttttgg atttgcagtg gaaacttatg gaaagctgac tatcggcgac ggacttgttt
     2341 ctcagattcc cgggcttttg gccaccacgg cgaccggtat gattgtgact cgggcagcat
     2401 ctgaaaaaga actttcgcag gaattcaaat ctcagatttt cagcaatcct aagactcttt
     2461 atgtagtttc cgcctgtctt gcactggctg catttattcc cggtcttcct atgttttctc
     2521 tgtttttctt ttccatcggt ctcgcgtatc tggcatacat catagacaga aatgcaaaag
     2581 agacaattac gagcatagag aaaaaagagg cagaaaacaa ggcggagaaa aaaccggact
     2641 attacaagga actcagaaca gatccgatcg aagtggagct tgggctgaat cttgttccgt
     2701 tggtaaatgc ctccttgggc ggtgttcttt tggaccagat tacaaacctg agaagacgtt
     2761 ttgccactga tacaggtctt gtcattcctg cagtcagaat tctggacaac cttgaactgg
     2821 atccggattc gtatgccatc aaaatacacg gtgtgattgt tggcgacagc aagatacgtc
     2881 ctactaaact gatggcaatt gacaattcag gaaaaagggc actcgatcag attatgggag
     2941 aggagttcct agagcctaca tttggattca aggcaaaatg gattgatcct gcagacaagg
     3001 tggatgccga aaacaagggt gcggctgtag tcgatccttc cactgtcatt gtcactcatt
     3061 tgaaggaact catctccaat catgcagctt cacttctcgg acgtgaggaa gtaaaaaatt
     3121 tacttgacca cgttaagacc tcatatccta ctttagtcag cgagatagat gaaaagaaac
     3181 ttggggtagg actgattcag caggttctgc agaatctgct cagagaagga cttggaatca
     3241 aaaacttagc ctccattttg gaagctatac tgaacaatga gcctaagacg agagatccgt
     3301 tctttctttc ggaaagtgtt cggcaggcga tttccagaca gattgtcact gattatctga
     3361 gcaaggatgg aaaactgcat gtagtgacga tggatcctaa ggtatctgaa aaactgagta
     3421 aatccatagt cgtggatccg attgagggaa gtctgatttc agtgcctcct gactactatc
     3481 ataaactgat agaaagcata gccagagaat acaacaggtt tcacagtgag ggaaggtttc
     3541 caattattgt gactagcagg gccttgagac ttccgctttc ctatatgctc gccaaagagt
     3601 ttccgcccag aaactttgca gttctggcaa tggaggaaat tcatgctgct gtgaaaacat
     3661 ctatggatgg aatcatcaca gtacagacag cagacccggt tcgtgccaga ggctcgaact
     3721 acgaatgagg gagtgacaga tggattttgt gaaaatcagg gggaaggaca ttcaggactg
     3781 ctttatgcag atgaaaatga aatatggacc tgaagcccac gtttatgagc agagaatagt
     3841 gaccgaaggc ggaattttcg gtactggatt tctcgcaaaa aaatatgtag aaatagaaat
     3901 cggagttcct gaacagcagt catccaaaga gaaagtggaa aaaaaacttc aggatctgaa
     3961 ggaacttctc aagcagaaat cagaagaaga agggcctaag aaaaaaactc tcacaaatat
     4021 gaaacctctt cttcagagag aaaaagaaaa ggaaaattcc aaacagaccg taaaggacga
     4081 ttatatcagt gacgattttc tggacaatga tgatgaagag gaggattata ttcctccgag
     4141 caatcttggt ctctccatta aagatgaact gactaaaaaa gagagtgtct ccgcatctgc
     4201 agagaaagaa aaatcctctc aaatttatga atcagtctat ctgaaaagaa tgaaggacaa
     4261 actcgtcaga gacggttttt ccaatgatta cgcagaggaa gtcatcagca aggcagacaa
     4321 atacctttcc tatgtagaca
//
LOCUS       DAOQGJ010000076         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_10930_length_4323_cov_3.221181, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000076 DAOQGJ010000000
VERSION     DAOQGJ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10930_length_4323_cov_3.221181"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PK683_01945"
     CDS             <1..947
                     /locus_tag="PK683_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH07236.1"
                     /translation="QRAAFEGKKRDIKSAFFQGKTGFFKDRVFILYGGRYDMYSDFGN
                     QLIPRAGLIFMFQENTALKFLYGQAFRAPNAQDLTSSTAVLGNPKIKPEIIDSYEIVL
                     MHKEKFWKTNLVLFENVWRNGIVRVLKPSLPQPFVAEAENKGVNKSRGAEIEFYFNNN
                     KWDFLLSGSHIISKDYTQPEFITVNQSNFNVAQNPAFFTSQNPNGKRYTLFPRYIFST
                     GIGYNFDWNKLTVFLIQRWFSNFRDVVYENNTGSHYQKNYNRFDLTVSVSPTENFEGG
                     FAVRNLTNRRNVVPGTYENSYGIYDEELNASVRMTVRM"
     gene            complement(948..1337)
                     /locus_tag="PK683_01950"
     CDS             complement(948..1337)
                     /locus_tag="PK683_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH07237.1"
                     /translation="MKLDIQILQNNGSTDTESVTVKLAGSLDTSTAPELENQLKNVFS
                     HPKKDLIFDLTDLKFISSAGLRVFAIARKVMKENSGETHFINMQPQIQAVFEIIKSLP
                     GVTVFKDMPEFDAYLAARQRSFTDKGR"
     gene            1477..1605
                     /locus_tag="PK683_01955"
     CDS             1477..1605
                     /locus_tag="PK683_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07238.1"
                     /translation="MYGNVSFAVAQTCGRTILSMQSSQGSFIGKTVAERCGEFTEL"
     gene            complement(1776..2216)
                     /locus_tag="PK683_01960"
     CDS             complement(1776..2216)
                     /locus_tag="PK683_01960"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07239.1"
                     /translation="MIIELNAVPEDIMKAVEILNQYCLEKKIPEKIIFSLKLVLEECG
                     SNIINHSLKNERDKKISVFMKKTDNLMIIELRDHGAEFDPTKTNFLENGNASASEGGT
                     VGGWGLKLVRQNIDEMHYIRSENTNILTLKKYLPLCYAERSPDF"
     gene            complement(2206..3360)
                     /locus_tag="PK683_01965"
     CDS             complement(2206..3360)
                     /locus_tag="PK683_01965"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07240.1"
                     /translation="MKNLSECRILIVDDSEENLSILVGGLKSDYRLSAVKSGENALKT
                     AARNPPDLVLLDIIMPGMDGYEVCRRLRKMPETAEVPIMFLSSLEDLQNKVIGFEAGA
                     NDYLTKPFEMLEVRARVHSLLKVKAYNDAVKEHIAAELKTAHEIQMGMLPHNFSELEK
                     AFNLEISAVLLPAYEVGGDLYGVHASDRNRLTVFLGDVSGKGIPASLFMIRAISIARL
                     LSRESNEPNQILSKLNDELCADNPYSMFVTFLCADYNPNTGILTVANAGQCRPVLLRK
                     GKPPVWAVSGLGTALGLEPGLKFEQTKISVQSGDAMLFYTDGVSEALNSNDDVYGSEQ
                     IITDADKFKNESASAITAGLLQRVHSFAGKVPQSDDIAILTLRFKNTYDN"
     gene            complement(3357..>4323)
                     /locus_tag="PK683_01970"
     CDS             complement(3357..>4323)
                     /locus_tag="PK683_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNH07241.1"
                     /translation="HPDLVFLDLRMPGMDGFEVCRLLKADSATQEIPVIFLTARTETE
                     DEKFGLDLGAADFISKPINPAILLARVKTQIRLKAASEFLKDENEFLEQEIRKRTKRI
                     ISTQDVVILALASLAETRDSDTGNHIRRTQWYVKYLASGLKKYSKYESFLTSSNIELL
                     FKSAPLHDIGKAGIPDSILLKPGKFETEEFEIMKTHPVLGKNAIEKAEKTLGAFLEFP
                     QIAKDIVLYHHEKWDGTGYPEGLSGENIPVSARLMAVADVYDALICRRIYKEPLPHEK
                     AMEIIISGRGKHFDPDIVEVFTEIHKDFYSISEDYSDENLNGLSF"
BASE COUNT         1140 a          930 c          921 g         1332 t
ORIGIN      
        1 ggcagagggc tgcttttgaa ggtaagaaaa gagatattaa aagtgcattc tttcaaggaa
       61 agacagggtt tttcaaggac agggttttta ttctttacgg cggaagatat gatatgtatt
      121 cggatttcgg aaatcagctt atccctaggg caggcctgat ttttatgttt caggagaata
      181 ctgctctcaa atttctttac ggccaggctt tccgtgctcc aaatgctcag gatctgactt
      241 cttccactgc agtgctggga aatccaaaga tcaagcctga aatcattgat tcgtatgaaa
      301 tcgtgctgat gcacaaggaa aaattctgga aaacaaatct ggttctgttt gaaaatgtct
      361 ggagaaacgg aattgtccga gtgctcaagc caagtctccc tcagccgttt gtcgcggagg
      421 cggaaaacaa gggggtaaac aagtcgaggg gagcagagat cgaattttac ttcaataata
      481 ataaatggga ctttttatta tccggttcac atattatatc aaaagattac actcagcctg
      541 aattcatcac agtcaaccag tccaacttca atgtggcgca gaaccctgca tttttcactt
      601 ctcagaatcc gaacggcaaa agatacactc tttttccgag atatatattc agcacaggga
      661 tcggatataa ttttgactgg aataagctga ctgtctttct gatacaaaga tggttttcca
      721 atttcagaga tgttgtttat gagaacaata caggatcaca ttatcagaaa aattataatc
      781 gtttcgattt gacagtctct gtttcgccta cagagaactt tgagggagga tttgctgtca
      841 ggaatcttac aaacagaaga aatgtcgttc caggaacata cgaaaactca tacgggattt
      901 atgatgaaga gctgaatgcc tcggtgagga tgactgtcag aatgtgatta tctccctttg
      961 tccgtgaatg aacgctgtct tgctgcgaga taggcatcga attcaggcat atccttgaat
     1021 acagtgactc caggcagaga tttgataatt tcaaacactg cctggatctg aggctgcata
     1081 ttgataaaat gtgtctcccc gctgttttcc ttcataacct ttcttgcgat tgcaaatacc
     1141 ctgaggcctg cgctgctgat gaactttagg tcggtcagat caaaaatcag atcttttttc
     1201 ggatgtgaaa aaacattctt aagctgattt tccagttcag gcgcggttga cgtatcaaga
     1261 cttcctgcaa gtttcactgt aacactttca gtgtcggtac ttccattgtt ttgcagaatt
     1321 tgaatatcca gtttcatact ataattttct gagactgcgt tttaatgtaa agcgatttta
     1381 ctgctgctct gtcaggctgc ataagactct cagagcttaa tgtcggccta aatatgcggg
     1441 tttctctctg ctgcattcga aaagtaaagc tgttggatgt atgggaatgt ctcttttgca
     1501 gtggcacaga cctgcggcag aacgattctg tctatgcagt caagtcaagg ttcattcatt
     1561 ggaaagacgg ttgccgagcg gtgcggtgag tttaccgaac tgtgagtttc actgcctatc
     1621 tcgacctgtg gtgaattgag tcgagtccat gcgagaaccc tgaaaatatt gggtttgtat
     1681 gtctcggctc gacacaccgc tgtcgctgct cgcgttgacg gcggtgaaaa ttgtatgctt
     1741 cgatatgacc cttcggcggc tcagggcatt gctcatcaaa agtcggggga tctttccgca
     1801 taacataaag gcagatactt tttcaaagta agtatatttg tattctctga gcgaatatag
     1861 tgcatctcat caatgttctg tctgaccagc ttcaggcccc atccgccgac agttcctccc
     1921 tcagaggcag aggcgtttcc gttttccagg aaattcgtct tcgtcggatc aaattctgct
     1981 ccatgatcgc gaagttcgat aatcattaaa ttgtcagttt ttttcatgaa aactgaaatt
     2041 tttttatccc tttcattttt cagagaatga ttgatgatat tgctcccgca ttcttccaga
     2101 acaagtttca gagagaaaat gattttttcg gggatttttt tctccagaca gtactgattc
     2161 aggatttcaa ccgctttcat aatgtcttcc ggaactgcat tcagctcaat tatcataagt
     2221 attcttaaac ctgagtgtca gaattgcgat atcgtccgac tgaggaactt tcccggcaaa
     2281 actgtgcact ctctgaagca gtcctgctgt tattgcggat gcggattcat ttttgaattt
     2341 gtctgcatct gttatgatct gttcgcttcc gtaaacatcg tcattggaat tcagggcctc
     2401 gctgactccg tcagtataga aaagcattgc atctcctgac tgcacgctga tttttgtctg
     2461 ttcgaatttc agtccgggtt caagtccaag tgcagtgccg agtccagaaa ctgcccagac
     2521 aggcggtttt ccttttcgga gcagaacagg tctgcactga cctgcatttg caactgtcag
     2581 aattcctgta ttcggattat agtcagcaca taggaatgtg acaaacatac tgtaagggtt
     2641 gtctgcacag agttcatcat tgagtttgga gaggatctga ttcggttcat tgctttctct
     2701 cgacagaagt cttgcgatgc tgatcgccct gatcataaaa agagatgcag gaattccctt
     2761 gcccgataca tctcccagaa atacggtcag tctgtttctg tcagaggcat gtacaccgta
     2821 cagatccccg ccgacttcat aagcaggaag aagaactgca gaaatttcca aattgaatgc
     2881 tttttcgagc tctgaaaagt tatgaggaag cattcccatc tgaatttcat gtgccgtctt
     2941 aagttcagct gctatatgtt ccttgacagc atcgttgtac gctttgactt tcagaagaga
     3001 atgaaccctt gctctcactt ccagcatttc gaagggttta gtaagataat cgtttgcccc
     3061 tgcttcaaaa ccgatgacct tgttctgaag atcctcgagc gaagaaagaa acataatcgg
     3121 aacttctgct gtttcaggca tttttctcag acgtctgcag acttcatatc cgtccattcc
     3181 aggcataatg atgtccaaaa gcacaagatc aggaggattg cgtgcagcag ttttcaaggc
     3241 gttctctccg cttttcactg cactcagtcg atagtctgat ttcaggccgc ctacaagaat
     3301 ggaaagattt tcctccgaat cgtcaacaat cagaattctg cactcagaaa gatttttcaa
     3361 aatgaaagtc cattaagatt ttcatcggaa taatcttcgg aaatactgta aaaatcttta
     3421 tgtatttcag tgaatacttc cactatatcc ggatcaaaat gttttccccg ccctgatatg
     3481 atgatttcca ttgctttttc atgaggcaga ggttctttgt atatccttct gcagataagg
     3541 gcatcgtata catctgcgac tgccatcagt cttgcagata ctggaatgtt ttctcctgaa
     3601 agcccctcgg gatatcctgt cccatcccat ttttcatgat gatataatac tatgtctttt
     3661 gcaatctgag gaaattccag aaaggctcca agtgtttttt ccgccttttc tattgcattt
     3721 ttacccagaa caggatgagt tttcattatt tcaaattctt cagtttcaaa ttttccgggc
     3781 tttagcagta tgctgtccgg aattcctgcc ttgccaatat cgtggagagg tgcggattta
     3841 aaaagaagct caatatttga actggtcaga aaggattcat atttggaata ttttttcaga
     3901 ccggatgcca gatattttac ataccactgt gttcttctga tatggtttcc tgtatcactg
     3961 tctctggttt cggcaagaga tgccagcgca agaatgacta catcctgagt tgatataatc
     4021 cttttagtcc ttttcctgat ttcctgttcc agaaattcat tctcgtcttt taaaaactca
     4081 gacgcagcct tcagtctgat ctgagttttt accctggcaa gaagaattgc aggattgatc
     4141 ggcttggaga tgaaatctgc tgcaccgaga tccagtccga atttttcatc ctcagtttca
     4201 gttctggcag tcagaaatat gacaggaatc tcctgagttg ccgaatcggc cttcaggagt
     4261 ctgcatacct caaacccgtc cattccaggc atgcgcaggt ccagaaacac cagatccggg
     4321 tga
//
LOCUS       DAOQGJ010000077         4301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11020_length_4301_cov_3.502826, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000077 DAOQGJ010000000
VERSION     DAOQGJ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11020_length_4301_cov_3.502826"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PK683_01975"
     CDS             <1..343
                     /locus_tag="PK683_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07242.1"
                     /translation="QDGRLTLEISFEPSQVTVKWLGESKEREPIKFISPVFEHIYSKD
                     SKNPIVMDFTKLEYMNSSTVTPIVKQIEHAKRENRKITIYYKKGLKWQELSFSALRVF
                     NIPGSVDVTGI"
     gene            349..1719
                     /locus_tag="PK683_01980"
     CDS             349..1719
                     /locus_tag="PK683_01980"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07243.1"
                     /translation="MLSILNIFKASPELERSMLVVDNFSDYLNALIHSWLKTLTILGF
                     TLVPIFFVLDIFIIPSDQQHLLPRFAVYRGFATAVVILQFFIISRTKPSNLSFLRGYI
                     FNFFVCGAIVLMTVDLGGFNSSYYAGLNLVVIAVNLLLPWKASHSAMNGLFTLLLYVG
                     MNAAFGKDFQLYNLINNLYFMSSTIIIAASINHVKHILIQKEFESRKDLKQARDALWS
                     EMEIAKQIQTALTPPDNTLGGYEMAAIMIPAEEVGGDYYDIIRTDKNPWVMIGDVSGH
                     GVESGLIMMMTQTSVRTVISENPTVSPSRLLVQLNKVIKENIALLKVDRYLTITAFSL
                     LPDKIIYAGSHQDILIARKSSAEVEALPTYGTWVGMLDDVEPFMQDRSFSLNPGDTAL
                     LFTDGITELGASEKKMYGDEKLKEGLKKYSHLPIKEIIDKIVKEAIAEFPKQEDDITL
                     VIIRKV"
     gene            1723..3150
                     /locus_tag="PK683_01985"
     CDS             1723..3150
                     /locus_tag="PK683_01985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HNH07244.1"
                     /translation="MVINEKRKTVRLRFHDEENCRIEISTGGKTYNGRVYDYSRFGLG
                     ISVAEKESAELHTGQKIDSCIIYAFGKKKELGTAALVRMESHNSEVLIGIFLDSEFVD
                     MDFLLTKQALFLQEDEIKKVKMHLSVQENLSQEFIEFCSHFTFALSLYKISLDELDAK
                     FMHEPERLKEALFNSLLQGIGKEFYQFLTDSMMELTAITAKYTKLENERSGFFLRKSV
                     FQFILESEFIKRTNIRPRGYAGDSVMMEMLYRNEYLGKSSFGKIFHKHACDTKAADAV
                     RNRRKLINHFMNETLSKSDKKEFRILSIACGPAWEIQDFLKDSPYRHKVKIHLLDQDD
                     EALNEAKNGILSIDNQGQPFSYEFIKESVRTILKTKSPELLFGQFDFIYSMGLYDYLT
                     DSVAKALTMKLFSMLSEKSVMIIGNYHETNETRKYMEYIMDWALYYREESSMLELLED
                     IQVPIQSFVDFDGSGTQMFLKVTKL"
     gene            3246..>4301
                     /locus_tag="PK683_01990"
     CDS             3246..>4301
                     /locus_tag="PK683_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH07245.1"
                     /translation="MSLLTDTASEMLYPIMPIYLKTIGFSVVLIGILEGLAEAAAGFS
                     KGYFGKLSDTSGRRVLFVQVGYALSAVSKPMMAVFIFPLWIFSARTIDRLGKGIRTGA
                     RDALLSEEATPQTKGKVFGFHRSMDTLGAVIGPVLALIYLYYNPEDYKTLFYIAFIPG
                     LSAVIASFLLKEKNKTTYETGSPIPFFSFLHYWKESSVAYKKLASGLLFFTLFNSSDV
                     FLLLKAKEAGLDDSALIGVYVFYNLVYALSAFPAGIIADIAGLKTVFVTGLFLFAAVY
                     TGMSISKDMYVFFGLFFLYGIYAASTEGISKAWISNITEKKDTATAIGTFSALQSICT
                     MLASSMTGLIWFYFGASV"
BASE COUNT         1264 a          828 c          935 g         1274 t
ORIGIN      
        1 tcaggatggt cgtctcactc tcgaaatcag ctttgaacct tctcaggtca ctgtaaaatg
       61 gctgggggag agcaaggaaa gagaaccgat aaaatttatc tcccctgtat ttgaacatat
      121 ttattcaaag gattccaaaa atccaatagt aatggatttc actaagcttg aatatatgaa
      181 ttcctctaca gtgactccta ttgtaaaaca gattgaacac gcaaagcggg aaaacagaaa
      241 gatcactatt tactataaaa agggactgaa atggcaggaa ctgagttttt ccgctttgag
      301 agtattcaat atccccggct ctgtggatgt gactggaatc tgatctgaat gctgtcaatc
      361 ttgaatattt tcaaagctag tcctgaactg gaaaggtcca tgcttgttgt ggacaatttt
      421 tccgattacc tgaatgcact gattcattcc tggctcaaaa ccctgactat tctaggtttt
      481 actcttgttc cgattttctt tgttctggat atttttatta ttccttcgga tcagcagcat
      541 cttcttcctc gttttgcagt gtacagagga tttgcaactg ccgtagtcat tcttcagttc
      601 ttcattatca gcaggaccaa gccttcaaat ctttcctttc tgcgtggata tatattcaat
      661 ttttttgtct gcggtgcaat tgttctgatg acagttgatc tgggaggatt caattcctcg
      721 tactatgccg gactgaatct ggtagtcatt gctgtaaatc ttcttcttcc gtggaaagca
      781 tctcactctg ctatgaacgg tctttttaca ctgcttctgt acgtaggaat gaatgccgca
      841 tttggaaagg attttcagct ctacaatctg atcaacaatc tgtattttat gagttccacg
      901 atcatcattg cagcgagcat caatcacgta aaacatatac tgattcagaa ggagtttgaa
      961 agcaggaagg atctcaaaca ggcaagagat gctctatgga gcgagatgga aattgccaaa
     1021 cagattcaga cagcactgac tcctccagac aatactctcg gcggttatga gatggctgca
     1081 attatgattc ctgccgaaga ggtgggaggt gattattacg atattatccg aacagacaaa
     1141 aacccttggg tgatgatcgg ggatgtttca gggcatggag tggaatccgg tctgatcatg
     1201 atgatgaccc agactagtgt cagaacagtc atttcggaaa atccgacagt cagtccgagc
     1261 agactgcttg ttcagctgaa caaggtcatc aaggaaaata tcgcacttct gaaagtggac
     1321 agatatctca caatcactgc gttctcgctt ctgcctgata agatcatata tgccggatct
     1381 catcaggaca tcctgattgc aagaaagtct tctgctgaag tggaagcact tcctacgtac
     1441 ggaacctggg tagggatgct ggatgatgtg gaaccgttta tgcaggacag aagtttcagt
     1501 ctgaatccgg gtgatacggc gcttttattt accgacggga ttactgaact tggtgcatcc
     1561 gagaagaaga tgtacggtga tgaaaaactg aaagaaggtc tgaaaaaata cagtcatctt
     1621 ccgatcaagg aaatcattga taagattgta aaagaggcaa ttgcagaatt tccaaaacag
     1681 gaagacgata tcactcttgt catcattcga aaggtataat ccatggtaat caatgaaaaa
     1741 agaaagactg tccggcttcg gtttcatgat gaggaaaact gcagaataga aatcagcaca
     1801 ggcggaaaaa catacaacgg aagagtgtat gattattcca gatttggact gggaatttcg
     1861 gtagccgaaa aagagagcgc agagctccat acagggcaga aaattgacag ctgcatcatc
     1921 tatgcatttg gaaagaaaaa ggaacttggt acagcagctc ttgtcagaat ggaatcacat
     1981 aacagtgagg tgctgatcgg aatatttctg gattcagaat ttgtggatat ggatttcctt
     2041 ctgacgaagc aggcactttt tcttcaggaa gatgaaatta aaaaagtcaa aatgcatctc
     2101 tctgttcagg aaaacctttc tcaggaattc attgaattct gttctcattt cacatttgct
     2161 ctttcactct acaaaatctc tcttgatgag ctggatgcta aatttatgca tgagcctgaa
     2221 aggctgaagg aagctctgtt caattctctt cttcagggaa taggtaagga gttttatcaa
     2281 tttcttactg attcaatgat ggaactgact gccattactg cgaaatatac caaacttgaa
     2341 aatgaacgtt cgggattctt cctgagaaaa tcagtttttc agtttatcct ggaatcggag
     2401 ttcatcaaga gaacgaatat caggcctaga ggttacgccg gcgattctgt catgatggag
     2461 atgctttatc ggaatgagta tctcggaaag tcctcctttg gaaagatatt tcataaacat
     2521 gcctgtgaca caaaggctgc cgatgcggtg agaaacagaa gaaaactcat caatcatttt
     2581 atgaatgaga ctctttccaa gtccgataaa aaggaattca gaattctctc aattgcatgc
     2641 ggtcctgcct gggaaattca ggatttcctg aaagacagtc cttacagaca caaggtaaag
     2701 attcatcttc tggatcagga tgatgaagca ttgaatgaag ccaagaacgg catactttcc
     2761 atagacaatc agggacagcc tttttcatac gaatttatta aagaatcggt cagaacgatt
     2821 ctgaagacaa aatcacctga gcttcttttc ggacagtttg attttattta ttctatggga
     2881 ctttacgatt atctcacaga ttcagttgca aaggccctta cgatgaagct cttttcaatg
     2941 ctcagtgaga agtctgtaat gatcatcggc aattaccatg aaacaaacga aaccagaaag
     3001 tatatggaat atataatgga ctgggcactg tattacaggg aagaaagctc aatgcttgaa
     3061 cttctcgaag acatacaggt cccgatccag tcatttgtgg attttgacgg atcgggaact
     3121 cagatgtttc taaaggtgac taaactttag gctgagtata tgaatacagt ataagtcaga
     3181 ttcctgccag taaacgttac tgaattttga attatattac acggacagtc tggattttat
     3241 ctgcagtgag tcttctgaca gacactgcaa gcgaaatgct gtatccaatt atgcccattt
     3301 acttaaaaac aatcgggttt tcagttgttt taatcggaat actggaagga cttgctgaag
     3361 cagcggcagg attcagcaag ggttacttcg gaaaactttc cgatacttca ggcaggcgtg
     3421 ttctgtttgt ccaagtcggt tatgctttga gtgcagtctc aaaaccaatg atggctgttt
     3481 tcatttttcc gctttggata ttctctgcca gaacaattga ccgtttagga aagggcatac
     3541 ggacaggagc aagagatgct ctgctttctg aggaagcgac accgcagaca aaaggaaagg
     3601 ttttcggttt tcatcgctca atggacacct taggagctgt aatcgggcca gtgctggctt
     3661 tgatatatct gtattataat ccagaagatt acaaaacact tttttatatt gcttttatac
     3721 cgggactttc tgctgttatt gcgtcctttc ttctgaagga aaaaaacaaa acgacttacg
     3781 agacagggtc tcccattccc tttttttcat ttctgcatta ctggaaagaa agttcagttg
     3841 cttataaaaa gctggcatca ggacttcttt tttttacact tttcaacagt tcagatgttt
     3901 ttctgcttct taaggcaaaa gaagcaggtc ttgacgattc tgcactgatc ggtgtttatg
     3961 ttttttacaa tctcgtatat gcactgtctg catttcccgc cggaattatt gctgacatcg
     4021 caggacttaa gactgtattc gtaacaggac tctttttatt tgcagcagta tatacgggaa
     4081 tgagcatcag taaggatatg tacgttttct tcggcttgtt ttttctttac ggtatttatg
     4141 cggcatcgac agaaggaatt tccaaagcat ggatttcgaa tattacagag aagaaagata
     4201 cagcaacagc catcgggaca ttctctgcac ttcagagcat ctgcacaatg ctggcaagtt
     4261 caatgacagg tctgatctgg ttttattttg gtgcatcggt c
//
LOCUS       DAOQGJ010000078         4283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11082_length_4283_cov_2.405866, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000078 DAOQGJ010000000
VERSION     DAOQGJ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11082_length_4283_cov_2.405866"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PK683_01995"
     CDS             <1..787
                     /locus_tag="PK683_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07246.1"
                     /translation="AELLDGRKMFVYDSEKNEYCIDDRFGYVVYCDPVTALQFLKENK
                     IIKTKTTDSIAAMMEWCRKHLQHFSGGDDLENLYDQWQYKGFPPLIRMIEGTVQLSDP
                     DSGKMHITYGCWGTTGFLKALLRIINIPVELVITAGHAIPRFVEEKLYLSHGDDLYNQ
                     LAFADPPIPIDKFLIEEEKFKKWFSGSDEENDKNIGRQVLELAIEYLPTCLLIDFGED
                     MEEEKDHSNGAVYQHFKNMYSVEALEKKGLWKKLNQRVYGKDE"
     gene            complement(844..2148)
                     /locus_tag="PK683_02000"
     CDS             complement(844..2148)
                     /locus_tag="PK683_02000"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07247.1"
                     /translation="MYSILRKKTEEEREINLQFHHYSYFLNFLRIRLFSMIFFFCNAV
                     ILVLNFINHSQGIGNKNPYSQYYIPAYLFFFIYFFIMSIIAYIQTPSQISEIRWRHRL
                     QVHIFALMMLVWISIITYIDLRVNGGITVYALGLLGLATMLFIHFFWGAALYSIAGGV
                     FYFLLISYEMKEDKMIADLLNGTIMIAISFIISQIIVKEKKKQFIKDSQIEKQKRELE
                     LINADLNAQNLKNAMLQMIIRHYIPQTTWKKADLNSSLSMPKIPEEDVDITCLFLDIV
                     SFTEYSEQHSPKEVIEKLNRVYAPIVNIIYDNGGDIDKFIGDAIFAFFEESQPALKSA
                     LEIFANLKNADAESSVEVRIGIHSGKVIRGDVGGKNRRDNTSIGDTVNIAARLQTACS
                     PNELIISEEAALSCRDFYEFKEKTSLSLKGKTLPINVFRIPG"
     gene            complement(2338..2748)
                     /locus_tag="PK683_02005"
     CDS             complement(2338..2748)
                     /locus_tag="PK683_02005"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNH07248.1"
                     /translation="MALLIEWNESLSVGVSEIDRQHMKLVGLINKLHHAMSEGHAKDV
                     MSQILDEMISYTDEHFKTEEKYFHQFKYIKTVEHMREHKNFVNTALQLQSDFKSGKLG
                     ISVGTMEFLKDWLTKHIQGSDKEYTKCFNENGLH"
     gene            3210..3980
                     /locus_tag="PK683_02010"
     CDS             3210..3980
                     /locus_tag="PK683_02010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH07249.1"
                     /translation="MVSAEEHYDKHLGKFYSWMIGEISENTQNQKIIFQKFGIVPRKS
                     SYAVDLGCGNGIQSIALADLGFSVCAIDFNDTLLSEFRSYADSEKIHIFRDRIENILK
                     YADKKQAGFGVSEGSPSRSAELIVCGGDTVSHLESFDVLEKFIEDCASSLCTGGKFFL
                     SFRDYSEELKENARFISVKNDESRILTCFLEYFREYVSVTDILYEKKENRWDQKISSY
                     RKLRISVEQMKSLLLRKNFRIEAVEKGRMNILIGAKAE"
     gene            complement(4061..>4283)
                     /locus_tag="PK683_02015"
     CDS             complement(4061..>4283)
                     /locus_tag="PK683_02015"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNH07250.1"
                     /translation="GADGAVTVTGNLDIKGISKSVSFPAKITYAADKKTGSAKANFNI
                     NRKLWKIEYPGKPDDLIRDEVNIDLDLAI"
BASE COUNT         1289 a          799 c          892 g         1303 t
ORIGIN      
        1 tgctgaactt ctggatggca gaaaaatgtt tgtatatgat tctgaaaaaa atgagtactg
       61 catagatgat agatttggat atgttgtcta ttgtgatcct gtaacagctc ttcagttttt
      121 aaaagagaat aaaatcatca aaaccaaaac gacagacagc atcgctgcta tgatggaatg
      181 gtgcagaaaa catctgcagc atttttcggg aggagatgat ctcgaaaacc tttatgatca
      241 atggcagtac aaaggtttcc ctcctctcat cagaatgatc gaaggcactg tgcagctgag
      301 tgatccggat tccgggaaaa tgcatattac atacggatgc tggggaacca ctggatttct
      361 gaaggccctt cttagaatca tcaacatacc tgtggagctt gtaattactg caggtcacgc
      421 cattccccga tttgttgaag aaaagctgta tctgtcacat ggagatgatc tctataatca
      481 gcttgctttc gcagatccgc ccataccgat tgataagttt ttgatcgaag aggaaaaatt
      541 taagaaatgg ttcagcggtt cagatgagga aaatgataag aatatcggca gacaggtgct
      601 cgaactcgca atagaatatc ttccaacttg tcttctcata gactttggag aggatatgga
      661 agaagaaaaa gatcacagca atggagctgt ttatcaacat ttcaaaaata tgtattcagt
      721 cgaagctctc gaaaaaaagg gactttggaa aaaactgaac caaagagtat atggtaagga
      781 tgagtgaatt tgatgtgagt cagtgagggc gcacagcagt gtgcccggaa cgagcggggg
      841 aaatcacccc ggaattcgga aaacattgat aggaagagtt ttgcctttca gagaaagaga
      901 tgttttttcc ttaaattcat aaaagtctct gcaggaaagt gcagcttctt cagaaataat
      961 cagttcattg ggactgcacg ctgtctgcag tcttgcggca atgtttacag tgtctccgat
     1021 cgatgtattg tcccgtctgt tttttccgcc tacatcaccc cgaatcactt ttcctgaatg
     1081 aatccctatc cgtacctcca cactggattc tgcatcggca ttcttcagat ttgcaaatat
     1141 ctccagcgca cttttcagtg caggctgact ttcttcaaaa aaagcaaata tagcatctcc
     1201 gatgaacttg tcaatatcac ctccgttgtc gtaaattatg ttcacaatcg gtgcatagac
     1261 cctgttcagt ttctcgatga cttccttggg agagtgctgc tccgaatact cagtaaatga
     1321 tactatatcc aaaaagagac atgttatatc cacatcttct tccggaattt tcggcatgga
     1381 aagactggaa ttcaagtctg cttttttcca ggttgtctgc ggaatatagt gccgtataat
     1441 catctgaagc attgcattct ttaaattctg cgcattcaga tctgcattga tgagttcgag
     1501 ttctcttttc tgtttttcta tctgactgtc ttttataaac tgttttttct tttcctttac
     1561 aatgatctga gatatgatga agctgatggc aatcataata gtgccgttca gaaggtctgc
     1621 tatcatcttg tcttccttca tttcataact gatgaggaga aaatagaaaa cacctcctgc
     1681 aatggaatac agtgcagctc cccagaaaaa atggatgaaa agcattgtgg caagaccgag
     1741 gagtcccagc gcataaacgg ttataccgcc gttcactctc aggtctatat aagtgatgat
     1801 actgatccag accagcatca tcagagcgaa tatgtgaacc tggagcctgt gtctccatct
     1861 gatttcactg atctgactcg gtgtctgaat gtatgcgata atgctcatta taaaaaaata
     1921 tataaaaaag aaaagatagg ccgggatata gtactgggag taagggtttt tattgcctat
     1981 cccctgggaa tggttgatga agtttaaaac aaggatgact gcattgcaga aaaagaatat
     2041 catactgaaa agtctgattc tgagaaaatt caaaaaatac gaataatgat gaaactgaag
     2101 gtttatctct ctttcctctt ctgttttttt tctcaaaatt gaatacattt tatttccttt
     2161 gatctgtaaa gagtccgaaa atacaaatcc gctttttgtg aaaagttgtc atcatatttt
     2221 aattagataa aattaaattt tccggatgga tcggagatgg cactccttga caatagttat
     2281 cctgtctgtg aatgcagacc tgcagttcag aaaagcagtc taatccgaaa aactgcatca
     2341 gtgaagcccg ttttcattga agcattttgt atactctttg tccgatccct gaatatgctt
     2401 cgtaagccag tctttaagaa attccattgt tccgactgag atacccagtt ttccgctttt
     2461 aaaatcggac tggagctgaa gagcagtatt cacaaaattt ttgtgttctc tcatatgctc
     2521 cacagtttta atatatttga actgatgaaa atatttttct tccgtcttga agtgttcgtc
     2581 agtataggaa atcatttcat ccagaatctg actcattaca tcttttgcgt gaccctcact
     2641 cattgcgtga tgcagtttat tgataagacc cacaagtttc atatgctgtc tgtcaatttc
     2701 ggaaacccct acactcagac tctcgttcca ttcgataagc agtgccatct tccctccctg
     2761 aatcgcagac tgaaaatacg actcaattca ctgtatgttc ttcagactga atatttgccg
     2821 actgagatgc aacctttctt tttttagatc attataagct ttatcatctg ataagtcaat
     2881 tgttttactt gcagggaaac gttacttatg tttcattgaa ttgaagatac cggtctattg
     2941 agaaaaacga tgttcactgc tgacttttta cccggcgatc agaagtcagc aatctggtcg
     3001 ggaaaaagtg tcaccttatg caaagaggat atgctatcta gaccctggaa ttttcaaaaa
     3061 gaattaggaa aatctaatga atcagtaaaa agggattttt aagggggggt atttcagcag
     3121 tgtcaaggtt cgggaaaccg tcggcaggct cacttgcaaa aacgcccctg cgtaagatga
     3181 gctaagaaca tatttttcag gagaatagaa tggtttcagc agaagagcac tatgacaagc
     3241 atttgggaaa gttttattcc tggatgatcg gcgagatcag tgaaaatact cagaatcaga
     3301 aaattatttt tcagaagttt ggaattgttc cgagaaagag cagctacgca gttgatctgg
     3361 gatgcggtaa cgggatacag tctattgctc ttgcggatct gggcttttct gtctgtgcaa
     3421 tagattttaa tgataccctt ctgagtgagt tcagaagtta tgctgattca gaaaaaattc
     3481 atatattcag ggacaggatc gaaaatatcc tgaaatatgc agacaagaaa caagccgggt
     3541 ttggagtgtc agaagggtca ccttcaagat ctgcagaact gattgtatgc ggaggggata
     3601 ctgtctccca tttggaatct tttgatgttc tggaaaaatt tattgaagac tgtgcctctt
     3661 ctttatgcac cggcggaaaa ttttttcttt catttcggga ctattcagaa gagctgaaag
     3721 agaacgccag attcatttcg gtgaaaaatg acgagagcag gattctcact tgttttctgg
     3781 agtatttcag agaatacgtt tcagtgacag atattcttta tgaaaaaaaa gagaaccgct
     3841 gggatcagaa aatcagttca tatagaaaac ttagaatcag tgtcgaacag atgaagtccc
     3901 ttcttttaag gaaaaatttc agaattgagg ctgttgaaaa aggcagaatg aatattctga
     3961 taggtgcaaa agcggagtga ctgtgaagtg ataaaaaaag ccctctgtaa cagagagcct
     4021 tttattatat aattatttac tgtaaaatca gatcgcgaga tcagatcgcc agatcgagat
     4081 caatgtttac ttcgtctctg atcagatcat caggttttcc tgggtattcg attttccaaa
     4141 gttttctgtt gatattgaaa tttgcttttg cagaaccggt ttttttgtca gctgcataag
     4201 tgatttttgc aggaaaactg actgatttgg aaattccttt aatgtcaaga tttccagtaa
     4261 ctgtaacagc accgtctgca cct
//
LOCUS       DAOQGJ010000079         4267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11158_length_4267_cov_3.404084, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000079 DAOQGJ010000000
VERSION     DAOQGJ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11158_length_4267_cov_3.404084"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2295
                     /locus_tag="PK683_02020"
     CDS             <1..2295
                     /locus_tag="PK683_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNH07251.1"
                     /translation="KDLQPSSGLLQESFEYLSTVREINPGYTVRRDLPASHDLSIYMP
                     PSGNQGQQASCSAWAVAYSVKSFYENRKRARDGWKLEIDRKPNYKAIFSPAFIYNQTN
                     NGKDQGIRLSDALRLTVTKGSLPWDTMPYNEKNFSSQPSLSQLELAASFRAEDFRKLR
                     YNNSSEIKTQIYQGNPVLAGILIDESFYKLRGKDIYKSHTGKNIIGHAIVITGYDDSL
                     QAFKFQNSWGQEWGESGFGYIDYKWFSKIGRSAFVLYDNTENSKFSSQLLDLNLSPDP
                     VFSERSPSDISASKGNFSDKVVITWAERKSAIGYEVFRSYPGENSYQQIGLSYTAYYE
                     DTGIIQDVAFSYKIKSVYENSISELSESSDIGYAVKTAGITPPKISGLKVSKGVFPDK
                     IYLEWQPLMNVTGYQVFKFDSRTRTFTSIGKTQIPSFTDRTASKNTVEIYTVAGLNNS
                     ITGELSDSASGYTRNPNLPSAPQNIKASLGEFPDKIEIRWDKVKDASGYFVYRFENGT
                     WTPRQYVTEEHYTDTEAGKGSKYYAVTAKSSNDIWSNFSEYAAGYADPKNARTGPRLN
                     PPRNVILSADRSSSEISIQWESAEKADEYNVWMKKYGDSEWKFTGKVSKEENSYRMKI
                     PEKNEFYLFAVTSKSDQFQESDFSRPASFVESESKKAVLKRNFGSLSKAEKFSGYWSG
                     MQWDGNLGVKMISMEITSVKDDLVSIKLDNKKAYEAKFVYESPVVEVEGKIRIKLSND
                     DALLVELKDKTLTKERVELSFLRD"
     gene            2492..2998
                     /locus_tag="PK683_02025"
     CDS             2492..2998
                     /locus_tag="PK683_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07252.1"
                     /translation="MEENIGDARKTYSFERLGECKARIKSYDDEKTVIVKLAKKKEPL
                     FLKGRFLESGQLHREAGTKVKNVNCESFKYGKFRMSDNNGSESLKDLNLLKLSLRKAE
                     FDSISGNVSFSRTETDACSVILSTSKPSEKYPDKDYLLEILNPGETRADYELYSVSVF
                     KAVKISSD"
     gene            complement(3019..3495)
                     /locus_tag="PK683_02030"
     CDS             complement(3019..3495)
                     /locus_tag="PK683_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07253.1"
                     /translation="MLRLFLYLLLFLAMQCKVPSVQSKTVLANSHIPDFWGKYGWNAS
                     PENLDKGLEDTEKGEWFYTMSTGEVRSENMKTSSKRFRTFLCGRDALQTGKAFFHKRI
                     AETEKLDESLAVEKMRQTDAFVYGVRECRPLEDSPQFVNCDCNIYVYIPGGRKIFK"
     gene            complement(3496..>4267)
                     /gene="metF"
                     /locus_tag="PK683_02035"
     CDS             complement(3496..>4267)
                     /gene="metF"
                     /locus_tag="PK683_02035"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNH07254.1"
                     /translation="NTVQDMKILNPGFISVTYGAGGSTKERTMDICQDIQSNFSIVSA
                     CHFTCVGADKSEILNSMQSIKSRGIDNVIALRGDPPKGQGKFVKTPGGFAHATELIQF
                     IRKEGFDFGIGGGCYPEKHPDSPTLQDDIRFLKLKADAGAEFLITQLFFSNSVFKNFI
                     KLAEMAGIKVPVIPGIMPITSFSQIERFRELAACHIPEDLVQELTEVKDNAEKFLQRS
                     LSFTVTQCRELLSMGVKGIHFYTLNQSTATVEILKELL"
BASE COUNT         1349 a          907 c          889 g         1122 t
ORIGIN      
        1 aaagacctcc agccaagttc aggtcttctt caggagtcct ttgaatacct ctccacagtc
       61 cgagaaatca atcctggtta cactgtacgc agagaccttc ctgcaagcca tgatctgagt
      121 atttatatgc cgccaagcgg aaaccagggt cagcaggcca gctgttcggc ctgggcagtt
      181 gcgtattccg taaagtcctt ttatgaaaac agaaaaagag caagggacgg atggaaactg
      241 gaaattgaca ggaaaccgaa ttacaaggcg atcttttcgc ctgcatttat ttacaaccag
      301 acaaacaacg gaaaagatca gggaataaga ctttcagatg cacttaggct tactgtgaca
      361 aaaggatctc tcccctggga cacaatgccc tataatgaaa aaaacttttc atctcaaccc
      421 tcactgtccc agcttgagct ggcagcatcc ttccgggcag aggacttcag aaaactccgt
      481 tacaataatt catctgagat aaaaacacag atttatcagg gaaatcctgt tcttgccgga
      541 atcctgatcg acgaaagctt ctacaagctt cgaggaaagg acatatataa atctcataca
      601 ggaaaaaaca tcattggcca tgccattgta attacaggat atgatgacag tctgcaggct
      661 ttcaaattcc agaattcatg gggtcaggag tggggggaat ccggattcgg ctacatagat
      721 tataaatggt tcagcaaaat cggcaggtct gccttcgtcc tgtatgacaa tacagaaaat
      781 tccaagtttt catctcagct tcttgatttg aacctgtctc cggatcccgt tttttcagaa
      841 cggtctcctt ctgatatttc tgccagcaag ggtaactttt cagacaaagt agtcataaca
      901 tgggctgaaa ggaaatctgc catcggatac gaagtattca gaagttatcc gggcgagaac
      961 agctaccagc agattggact ctcgtacact gcgtattatg aggatacagg catcattcag
     1021 gatgtggcat tttcctataa aataaaatct gtatatgaaa actcgatatc cgaactgagt
     1081 gaatcctcag atatcggcta tgcagtaaag acagcgggaa taactcctcc taaaatctca
     1141 ggactgaaag tcagcaaagg agtttttcct gataagattt atctggaatg gcagcctttg
     1201 atgaacgtga cagggtacca ggtttttaaa tttgacagca ggacaaggac atttacatca
     1261 ataggcaaga cacagatccc aagtttcact gacagaacgg catcaaaaaa cactgttgag
     1321 atttacactg tcgccggtct gaataattcg atcactggag aactttcgga ttccgcatcg
     1381 ggatatacca gaaatccaaa ccttccttct gcaccgcaga atataaaagc cagtctggga
     1441 gaatttccgg ataaaattga aatacgctgg gataaagtaa aggacgcttc cgggtatttt
     1501 gtgtacagat ttgagaatgg cacatggaca cccagacagt atgtgactga agaacactat
     1561 actgatacgg aagcggggaa agggagcaag tattatgcag tgactgctaa aagcagcaat
     1621 gacatatgga gcaatttttc tgaatatgca gccggttatg cagaccctaa aaatgcaaga
     1681 acaggcccaa gattgaatcc tccaagaaac gtaatactct ctgctgacag aagctcttct
     1741 gaaatcagca ttcagtggga atctgccgaa aaggcggatg aatacaatgt atggatgaaa
     1801 aaatacggtg attctgaatg gaaatttacg ggaaaggttt ccaaagaaga gaattcgtac
     1861 agaatgaaga tcccggaaaa aaacgaattc tatctttttg cagtaacatc aaagtccgac
     1921 cagtttcagg aaagtgattt ttccagaccg gcttccttcg tagaatctga atcaaaaaaa
     1981 gctgtattga aaaggaattt tggatcactt tcaaaagcag aaaaattttc cggctattgg
     2041 tcgggaatgc aatgggacgg aaatctggga gtaaaaatga tttctatgga aattacatca
     2101 gtcaaagacg atcttgtatc aattaaactt gataataaaa aagcgtatga agcgaaattt
     2161 gtatacgaat cgccggtcgt tgaagtagag gggaaaataa gaataaaact ctcaaatgat
     2221 gatgcacttc tggtggaact taaggataag acccttacga aggaaagagt ggaactttcc
     2281 tttctgagag actgagatga tgactttccg caaattttct tctctgcagg cattcgtatt
     2341 tctgctgtca gtctgtgctc tatcctctgc ttatcagaca gtcctgtacg catctgaaag
     2401 cacctgtgac tatttttttt ccggcaagtt aaagctctat cctgcggact catttagaaa
     2461 tgctcctgaa attcttttcc atgaaggctt aatggaagag aacataggtg atgccagaaa
     2521 gacttattcc tttgaaagac ttggagagtg caaggcaaga attaaatcat atgatgacga
     2581 aaaaacagtc attgtcaaac ttgcgaaaaa gaaagagccg ttatttctga aaggaagatt
     2641 tctggaatct ggacagcttc atagggaagc gggaacaaag gtaaaaaatg tcaactgtga
     2701 atcattcaaa tacggcaaat tcagaatgtc ggacaataac ggatcagaat cactcaagga
     2761 tttaaatctt ctgaaactga gccttcggaa agcagaattt gacagcatat ccggaaatgt
     2821 aagcttcagc aggacggaaa cggacgcctg ctctgtaatc ctcagcactt cgaaaccttc
     2881 tgaaaagtac ccagacaagg actatcttct ggaaatactg aatcccggag aaacaagagc
     2941 tgattacgaa ctgtattccg tgtcggtctt taaagcagtc aaaatcagca gcgactgatt
     3001 tctgactgcc attgctgatc acttaaaaat cttccggcca cctggtatat aaacgtaaat
     3061 attgcagtca cagttaacaa attgaggaga atcttccaga ggcctgcatt ctctgactcc
     3121 ataaacaaat gcatctgtct gcctcatttt ctccacagcg agcgattcat caagtttttc
     3181 cgtttcagca atacgcttgt gaaagaaagc ctttcctgtc tgaagcgcat ctctcccgca
     3241 tagaaaagtt cgaaaccttt tcgatgatgt cttcatattc tcagatctga cttctccagt
     3301 tgacatagtg taaaaccatt cacctttttc ggtatcttca agccctttgt ccaggttttc
     3361 gggagatgca ttccagccgt atttccccca aaaatccggt atatgtgaat ttgccaacac
     3421 cgtcttactt tgaacggaag gcaccttgca ctgcattgcc aaaaacagga ggagatacag
     3481 aaacagtctc agcattcaga gcagttcctt cagtatttcc actgtggctg tagactgatt
     3541 cagtgtataa aagtgtatac cttttactcc catagaaaga agttccctgc actgcgtaac
     3601 agtgaagctg aggcttctct gcagaaattt ctcagcatta tccttcactt cagtcaattc
     3661 ctgaactaag tcttcaggaa tatgacaggc cgccagttcc ctgaatcttt caatctggga
     3721 aaaacttgta atgggcataa ttccaggaat gacaggaact ttaattccgg ccatttcagc
     3781 gagctttata aagtttttaa agacactgtt tgagaaaaaa agctgagtaa ttagaaattc
     3841 tgcaccggca tctgctttga gtttcaaaaa ccgaatatcg tcctgcaggg taggggaatc
     3901 aggatgtttt tcgggataac atcctccacc gatcccaaaa tcaaaacctt ccttgcgaat
     3961 aaactgaatc agttcagttg catgagcaaa tcctccagga gtcttcacaa actttccctg
     4021 tcctttggga ggatcgcctc ttaaggcaat cacattgtcg atgcctcgcg atttaataga
     4081 ctgcatcgaa ttcagaattt ccgacttatc cgcaccgaca catgtgaaat gacaggctga
     4141 tacaatcgaa aaattactct gaatatcctg acagatatcc atcgtcctct ctttagtcga
     4201 tccgccggca ccatatgtaa ctgaaataaa tccagggttt aaaatcttca tatcctgaac
     4261 ggtattc
//
LOCUS       DAOQGJ010000080         4244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11261_length_4244_cov_3.556219, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000080 DAOQGJ010000000
VERSION     DAOQGJ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11261_length_4244_cov_3.556219"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK683_02040"
     CDS             <1..435
                     /locus_tag="PK683_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH07255.1"
                     /translation="CRFIFFKNVASAVGVILEHQKRILLITRARDPGKGLYDVPGGFC
                     DEWETPEETAGREIKDELNLNVSNFQYLASFPNRYLYKNILYHTMDMIYLCRLGSAEG
                     LKLEEEEVHHFEWFSKEEIPLEKIAFPSIRKALSLYIDGSSF"
     gene            complement(474..1064)
                     /locus_tag="PK683_02045"
     CDS             complement(474..1064)
                     /locus_tag="PK683_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07256.1"
                     /translation="MKDSAFKSAVILTVFLSSVISADDPQYTVESEVKSGVCTVTLKK
                     KEKAVKVFNGKELGVKSNDNCPFTELLSSSDGHFWISSGFTAGIDVISKIDSGTFKIT
                     KFDLTGLRLHSKEFALNPEKEKIAFSDYPLIFDVERANALLKKKFTLFIYDLKTKKKQ
                     AVATSVGKDFSPGWTDQKTLEYNNPKGDDRIQKAVP"
     gene            1694..3262
                     /locus_tag="PK683_02050"
     CDS             1694..3262
                     /locus_tag="PK683_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07257.1"
                     /translation="MDISPPVSAVISNSAISADIPSSVSLAALAPTLEITGTSILADG
                     KVQENGKTNNDFRKPVSYSILAEDGSKSDYTVTLNNTGFSILGSSGNVNITGLSASDA
                     ALEMYYDTSGNYFSAFDSNTKIGTVLPNTTNFALSFKSMPTGKTCSLTAGTTSGILSS
                     DVSFSVNCVSGYLVGGKILSRLSTFTVGANSASVTTVAGSMPPAASPGTTDGIGGAAR
                     LSTPVGVTSDGKDLYIADSGNHCIRKYEISTGNVTTLAGLCGTSGTADGTGTSARFNF
                     PIQISSDGTNLFICDRANNMIRKTEISTGNVTTLIGSGGASGDQNGIGTSARINFPNG
                     ITTDGNYLYISDRGNNKIKRMMISSGVVTTVAGDGTPASVDSANGLTASLNVPCDSVS
                     VSGILYFVDCNGNTLRKIQLSSPFPVSTIAGSGAAASIDGTGLSASFNNPHGIETDGT
                     NLFITEYNGKKIRRVGISGLNTVTLAGSAGYQDGTGTGAFLGNLGGITTDGNRLYFVD
                     PTNHALRRIDNPPD"
     gene            3618..>4244
                     /locus_tag="PK683_02055"
     CDS             3618..>4244
                     /locus_tag="PK683_02055"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HNH07258.1"
                     /translation="MKLRNCLLSAAVILSALNCLKADKSPFDISSKSPVSSGFAVLSL
                     LGASSSSKNQASETAAPSNISYGSQSSFNLIPGVAISYKPTVTGTVDSWSISPALPTG
                     LTFNSQTGEISGTPDALSYLASGFPLTTFTVAAINSGGTQNFLISLKILASGENVWTV
                     ISGTAGQNIGVNQGSLTVDNTSDTTSLLISGLISGNFDGETDPSSGAGA"
BASE COUNT         1250 a          964 c          887 g         1143 t
ORIGIN      
        1 tgccgcttca tattcttcaa aaatgttgcg agtgctgtcg gagtgatcct ggaacatcag
       61 aagaggattc ttctgattac aagggcaaga gatcccggaa aaggacttta tgacgttccc
      121 ggaggattct gtgatgaatg ggagactccc gaagaaactg ccggaagaga aatcaaggat
      181 gaactgaatc tgaatgtttc aaattttcag tatctggcat cctttccaaa ccgttattta
      241 tataaaaata tactgtatca cacaatggat atgatatatc tctgcaggct gggttctgca
      301 gaagggctta agctggaaga agaggaagtt catcattttg aatggttttc aaaagaggaa
      361 attcctctcg aaaagattgc atttccctcc atacggaaag cactgtctct ttacattgac
      421 ggcagctctt tctgaaatag aaggattgac cttttatccg atcaggggca ggtctatgga
      481 actgcctttt ggattctgtc gtctcccttc gggttattgt actccagagt cttttgatcg
      541 gtccagcctg gagaaaaatc ttttccgacg gaagtcgcga ctgcctgctt cttttttgtc
      601 ttcagatcat agataaaaag agtaaacttt ttcttgagaa gagcatttgc tctttccaca
      661 tcaaatatca gaggatagtc actgaatgca attttttctt tttcgggatt cagggcaaat
      721 tccttcgagt gaagccgaag gccggtcaga tcaaattttg taatcttaaa agtgcccgaa
      781 tcaattttgg aaattacatc aatgcctgca gtaaaaccgg aactgatcca gaaatgaccg
      841 tctgaagaag aaagaagttc ggtaaaaggg caattatcat tactttttac cccaagttcc
      901 tttccattga acacttttac agccttttct ttttttttca gagttactgt gcatacgcct
      961 gacttgactt ctgattccac agtatactgt gggtcatctg cactgatgac tgaagacaga
     1021 aatactgtaa gaattactgc tgatttaaac gcagagtctt tcatcttatt accttcctgt
     1081 tcttcagtat tttcactgag atactgtttg aaagcaaaaa acttcaggca tatctgctga
     1141 actggaagaa tatttacaga aagattcatt tcattttata tccggatttt ctgtcctatt
     1201 tcactcgtcc agaaatcaat acattataag acaaatatag tataatatta cttgacagat
     1261 ttcctgaagt ctcacagccc tgacccctcc tccccttcct gtaatacggc agtccatact
     1321 gaatcaggtg tcggatatac ggggatattt acagcactct gttcaagtct gcagtgaaca
     1381 gggtcggcag aaacggaata cagacccagt catcagaaaa gattcgacag atgactgcag
     1441 gcagaattca ttcccggcat tttagaacaa tcagccaggc ccagagatct gaaaatctca
     1501 tatcaatact tttttttcgt tgaaaattgt attcaacagt gtccaatgaa ttatgcgact
     1561 ggtcttatta ctgctttctg tcggcttttt tttcggctgt ataaaaagaa gcacaggact
     1621 gaaatttctg cttccgcaga cagctctcaa taaaaggctc tccagcgaaa aggagctgaa
     1681 gagttttaaa tttttggata tatcccctcc tgtctctgca gtcatttcaa attccgcaat
     1741 cagtgcagat attccctcca gtgtttcgct tgctgccctg gcaccgactc tggaaattac
     1801 cggcacctcc attcttgccg acggaaaagt gcaggaaaac gggaagacca acaatgattt
     1861 ccgcaagccg gtgagttatt caatactcgc agaagacggt tcaaaatcgg actatactgt
     1921 tacactgaac aataccgggt tcagtattct gggaagttcc ggaaatgtaa acatcacagg
     1981 gttgagtgca tcagatgccg ctctggaaat gtactatgat acaagcggga attacttcag
     2041 cgcctttgac agcaacacca aaatcggaac cgtccttcca aacacgacaa actttgcact
     2101 cagctttaaa tctatgccga caggaaagac ctgttctctg actgcaggta ctacatcagg
     2161 aatactttcc tctgatgtaa gtttttcagt gaactgtgtt tccgggtacc ttgtgggagg
     2221 caaaattctg tcccgacttt ccacattcac agtcggagca aattcagctt cagtaactac
     2281 agttgccggc tcaatgcctc ccgcagcatc accgggaact actgacggaa tcggaggagc
     2341 tgcccgactc agtacaccgg tcggagtcac ctcagacgga aaggatcttt acattgcaga
     2401 ctcaggaaat cactgcatcc gaaaatatga aatttccacc ggaaatgtga cgactttggc
     2461 aggactctgc ggaacctccg gcactgcaga cggaacaggg acctcagcaa gattcaactt
     2521 tcctatccag atatcttctg acggaacaaa tctttttatc tgcgacagag cgaataatat
     2581 gatacgtaaa acagaaatca gcacaggcaa tgtcacaaca ctcatcggtt cagggggtgc
     2641 atccggagat cagaacggga ttggaacttc agccagaatc aatttcccga acggaatcac
     2701 taccgacgga aattatctct atatcagtga cagaggaaac aataagatca agagaatgat
     2761 gatttcttca ggagtcgtca cgacggttgc aggagacgga actcctgctt ccgttgacag
     2821 cgcaaacgga ctgacggcaa gcctgaatgt gccctgcgac tctgtctctg tcagcggaat
     2881 tctctacttc gtagactgca atggaaacac tctcaggaaa attcagctga gttctccctt
     2941 tcctgtcagt acaattgcag gaagcggagc agccgcatcg attgacggaa caggtctcag
     3001 cgcctcattc aacaatccgc atggaattga aacagacgga acaaatttat tcatcacaga
     3061 atataatgga aaaaaaatac gaagagtggg catatccgga ctgaacacgg tcactctggc
     3121 cggttcagca ggctaccagg acggaacagg cacaggtgca ttcctgggaa atcttggagg
     3181 aatcactacc gacgggaaca ggctctattt tgttgatccg acaaaccatg ctctgagacg
     3241 gatcgacaat cctcctgact gagatgatct gtatctcctc ccctttttcc agaaaagtct
     3301 tcctgtattc ttcattcaga aaaaattcag gaccttcccg gctttcccct gcgcccgata
     3361 ttttcaaaga gcacccaatc ctcagacaga aaatcaatgt ataattatat aatatagaat
     3421 aaaatgaata attataccag aagactgatt ggcttgcaga aacagacttg gaatatcttt
     3481 aaaaatataa atgaaatcgg atgccccgtc ctgtcttttt tccctttttg gaactttttt
     3541 tcttaaaatg attcaaaaaa agggaatcag tagaaaaatt attgcaaaaa gaaaaaggac
     3601 agactgtagt accggatatg aaactgagaa actgtctcct gagtgcagca gttattctgt
     3661 ctgccttaaa ctgcctgaag gcagacaagt ctccctttga catcagcagc aaatcccccg
     3721 tgagttcagg atttgcagta cttagcctgc tgggagcatc atcttcttca aaaaatcaag
     3781 cttcagagac agctgcccca agcaatatca gctatggatc acaaagttca tttaacttga
     3841 ttcccggagt tgcaatttcc tataaaccca ccgttacagg cacagtcgat tcctggagca
     3901 tcagtcctgc gttgcccacg ggactgacat tcaattctca gacaggagaa atctccggaa
     3961 ctccagatgc tttatcctat cttgcctccg gattccctct gacaactttt actgttgcag
     4021 caataaattc aggaggcacg cagaattttc taatcagtct gaagatactg gcttcggggg
     4081 aaaatgtatg gactgtcata agcggaacag cagggcagaa catcggagtc aatcaaggga
     4141 gtctgacagt ggataatact tcagacacca catccctgct gatttcagga ctgatttctg
     4201 gaaattttga cggagaaaca gatccttctt caggagcagg cgct
//
LOCUS       DAOQGJ010000081         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11272_length_4242_cov_3.250060, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000081 DAOQGJ010000000
VERSION     DAOQGJ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11272_length_4242_cov_3.250060"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="PK683_02060"
     CDS             complement(<1..1461)
                     /locus_tag="PK683_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNH07259.1"
                     /translation="METRGTQEKKGFSKSFFDKLKISFSVPRMSRKISTLTVMIIFLS
                     CASGRSAELKKEDIRSEAGSGASADDFRNRTVKDSVLIETELNRKSQEYFDQLYRSVD
                     YDRLVEDDRLTLMKKVANKLSVAMGYGYYNESNIYLVKYKKDTSALNAFVLPTGQIFV
                     FEALVDLLKQKARENSNGSADDEQRILENLLAAVIGHEIGHYYGKHQLKSELRKLGNL
                     SSDEKLEKIKKTDLSKAQILIDDIENEQNYEIQSDWFAVTAMAKAGYEPGFLESLLYI
                     LKEYVPDRNPYLSTHPSMNRRIAYISKDPEKQKNLVDRIVRLEYAFAAVETGYKLDDA
                     AIAVKQELDRMPDNPYLLSALAKIYHRIWEKSCEISELRFKSSIIYSPFQEGMIHSVT
                     RGKEGLKRKFWCSRSDYETSLQYYRDAIKNFAGHHTLTSYAVLLSYHPDKEQYSSAVI
                     YARRAFEEISEEDSIGKLRALNNLAIVFFMNEKFEDS"
     gene            complement(1565..2722)
                     /locus_tag="PK683_02065"
     CDS             complement(1565..2722)
                     /locus_tag="PK683_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07260.1"
                     /translation="MKLLMNFRQINKSVLQRGFIGQEAGRVTGISSLKRMAVCFLILF
                     SFSSCFKKALFDNPFYGIMGIYYSIFQTPDKEIGYYSFERANNPGLVRNISSDILGKR
                     VRMYVPYGTQLESMVATYEYKAKQVDIGEAAQISGKTSNDFRKDVIYRVTANDDTKTD
                     YTVIIDKGSIDSKRMKTFGFLQKQNNIVRDSYGIFIGYTINVILPYGASLNPLIAKFT
                     SDGSDITVNGLTQNSESTSNDFEKPITYRVHAADGSYQDYPVSAAVASKDSKEILYFV
                     FTQDANFGNLNGDFFTTIVGNSISVTLPTNTNLKNLVASFVSSGRTISVNGATQRSNI
                     TANDFTKTVIYTVTAADDTSVDYAVTVKLQPYVPVYCLWDISSFDSECLWE"
     gene            complement(2734..3405)
                     /locus_tag="PK683_02070"
     CDS             complement(2734..3405)
                     /locus_tag="PK683_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNH07261.1"
                     /translation="MKRYIYKLKKGIFTGVGIALGTGIGTIALYAVSVTLNTFMAGTP
                     INAAKMNENFTNLKTAVDAAVPAGTVFAYTGTTAPDGFLICNGNAYPKSSYPALAALL
                     ETATGSRFGAPDISTFRVPDLRGRFLRGVNDASGNDPDASGRYALYTGGSDQDNIGSY
                     QDDMLKSHTHSYISNGGTDNDQDASGPGNGMGWPSSFQTGSSPTLGGNETRPKNVYVN
                     FIIKY"
     gene            complement(3410..>4242)
                     /locus_tag="PK683_02075"
     CDS             complement(3410..>4242)
                     /locus_tag="PK683_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07262.1"
                     /translation="CFGRARDRYKKENFMNEQYIREIFEKYYMERRNEERKKMLKKIL
                     IMMTCILFLIPLSRWAVSVAKPYAFSSGTAVNSSQVNANFDALFTKVNELDTRLTSLE
                     SGGKSWKLICEIDITSATTSFNTGDYGCNLNGNTDVEYNIITKIQSSAGGGSMFLIFP
                     NNDSVGGNYFMQGLYNLTGQGTNVYGDPANYGGIFIGNTAALNELIQSNSFFYAKSGK
                     IRNLAATVSGRMNSGTLYAGLYSTSWLNTSSNITNLQFLATAVNGIGPDSHFEIWARR
                     "
BASE COUNT         1161 a          970 c          775 g         1336 t
ORIGIN      
        1 agagtcttcg aatttttcat tcataaaaaa gacaattgcc agattgttca aagctctcag
       61 ttttccaata ctgtcttctt ctgaaatttc ttcaaaggct cttctggcat agataacagc
      121 agaactgtac tgttctttgt ccgggtgata agaaagcaaa actgcgtaag acgtcaatgt
      181 atgatggcct gcgaaattct ttattgcatc cctgtaatac tgcagagacg tttcataatc
      241 actccgactg caccagaact ttctcttgag tccttccttt cctcttgtaa ccgaatgaat
      301 cattccctcc tgaaaaggag aatagatgat cgaagacttg aaacgcagtt cagaaatttc
      361 acaggatttt tcccagatcc tatgataaat ttttgccaat gcagaaagca gatacggatt
      421 atccggcata cggtctaatt cctgttttac tgcgatagcc gcatcatcaa gcttgtatcc
      481 ggtttccacc gcagcaaagg catattccaa tctgacaatt ctatctacaa gatttttctg
      541 tttttccgga tcttttgaaa tgtatgcaat tctccgattc atagaaggat gagtggacag
      601 atacggattt ctgtcgggaa catactcttt gagtatatat aataagcttt ccaaaaagcc
      661 aggctcataa cctgcttttg ccattgcagt tacggcaaac cagtcagact gaatttcata
      721 gttctgctca ttttcaatgt catcaataag aatctgtgct ttgcttaaat cagttttttt
      781 aattttctca agcttctcat ctgaactgag gtttcccagt ttacgaagct ctgacttgag
      841 ctgatgtttt ccgtaataat gaccaatttc atgtccaata acagcggcca gaagattttc
      901 cagaattctc tgttcatcat ctgctgatcc gttcgagttt tctctggcct tctgtttcag
      961 aagatccaca agggcctcaa atacaaaaat ctgtcccgta ggcagcacaa aagcattcag
     1021 tgcagacgtg tccttcttgt attttaccag atagatattg ctttcgttat aatagccgta
     1081 tcccattgca acagacagct tattggcaac ctttttcatc agagtcagtc tgtcgtcttc
     1141 tacaagtcgg tcatagtcca cacttctgta cagctgatca aaatattcct gactttttct
     1201 gttcagttcc gtctctataa gtacactgtc tttgactgtt ctgtttcgaa aatcatctgc
     1261 agaagcgcct gaacccgctt ccgacctgat gtcttctttt ttcagttctg ccgatctgcc
     1321 cgatgcacag ctgagaaaaa taatcatgac tgtaagtgtt gatatttttc tgctcattct
     1381 cggtactgag aagctgattt tcagtttatc aaaaaatgat ttactgaatc cttttttctc
     1441 ctgggtccct cttgtttcca tttttacctc catagtaaag agtgaaattt attcctaacg
     1501 taattttaga aattgacagc ctttgaggct gtttaccttc gaatcggatt tatatgcatt
     1561 ctcctcattc ccagagacat tcactgtcaa atgatgatat atcccaaagg cagtacacag
     1621 gcacataagg ctgcagcttg actgtgactg cataatctac gctggtatca tccgctgcag
     1681 tcactgtata aatcaccgtc ttagtaaaat cattcgcagt aatattgctt ctctgtgtag
     1741 caccgttcac actgatagtc cgcccgcttg atacaaagga ggcgaccaga ttttttaaat
     1801 ttgtattggt cggcagagtg acactgatac tgtttcccac aattgttgta aaaaaatcac
     1861 cgtttagatt tccaaaattt gcatcctgag taaatacaaa atacagaatt tcctttgaat
     1921 ccttgctggc aacagccgca ctcactggat aatcctgata tgatccgtca gcagcatgga
     1981 ctctgtatgt aatcggtttt tcgaaatcat tgcttgtgct ctcactgttc tgcgtcagcc
     2041 cgttcactgt aatatcagaa ccgtctgagg tgaattttgc aatcagggga ttcagcgatg
     2101 ctccgtaagg cagaatcaca ttgatcgtat acccgataaa aataccgtaa gaatcgcgga
     2161 caatattgtt ctgcttctgc aggaaaccaa atgttttcat tcgtttcgaa tcaatagagc
     2221 ctttatctat aatgacagta taatcagttt ttgtatcatc attggctgta acccgataaa
     2281 ttacgtcttt ccgaaaatca ttgcttgttt ttcctgagat ctgtgcagcc tctccgatat
     2341 cgacctgctt tgccttatac tcataggttg ccaccatgga ctccagctgt gtgccataag
     2401 gtacatacat cctgaccctt tttcctaaga tatccgaaga aatatttctg accagtcccg
     2461 gattgtttgc ccgttcaaaa gagtagtacc caatttcctt gtcaggtgtc tgaaatatac
     2521 tgtagtaaat tcccatgatt ccgtaaaatg gattgtcaaa aagagccttc ttgaagcagg
     2581 aagaaaagga aaaaagaatc aggaaacata cagccatacg cttaagggag gagattcctg
     2641 taactctgcc ggcttcctga ccaatgaagc ccctctgcaa tactgatttg tttatttgtc
     2701 tgaaattcat tagtagtttc ataaaaatct ccatcaatat tttataataa aatttacata
     2761 cacgtttttt ggacgggttt catttccgcc aagtgttgga ctagatcctg tctgaaaact
     2821 agaaggccag cccattccat ttccaggacc tgaagcatcc tgatcgttat ccgttcctcc
     2881 atttgatata taggaatgtg tatgactttt caacatatca tcttgataac tcccaatatt
     2941 atcttggtca ctccctccag tatagagcgc ataacgaccg gaggcgtcag gatcatttcc
     3001 ggatgcatcg ttaactcctc gcaaaaatct gcctcgtagg tcaggaactc ggaatgtaga
     3061 aatatccggt gcaccaaaac gcgaaccagt tgcagtttcc aataatgcgg ccaaagccgg
     3121 ataactggat ttgggatatg cattcccatt gcagattaaa aatccatcag gtgctgtagt
     3181 gcctgtatat gcgaacacag taccagccgg gacagccgca tccactgccg tcttcaaatt
     3241 cgtaaaattc tcattcatct tggcagcgtt gatcggcgtt ccagccataa aagtattcag
     3301 agtcacgctg acggcataga gggctattgt cccgattcct gtcccaagag caattcccac
     3361 tcccgtaaaa attccttttt tcagtttata aatataccgt ttcatgattt tatctccttg
     3421 cccagatttc gaaatgagaa tcaggtccta ttccgttgac tgccgttgcc aaaaactgca
     3481 aatttgtaat gttgctagag gtattgagcc aggatgtaga ataaagtcct gcataaagtg
     3541 taccggaatt cattctgcct gatacggttg cggctagatt tctgatcttt ccagattttg
     3601 cataaaagaa tgaattgctt tgaatcaatt cattgagagc agcggtattg ccaataaaaa
     3661 tacctccata gtttgccgga tctccataca cgttcgttcc ttgtcctgtc aaattataca
     3721 gcccctgcat aaagtaattt cctccgacgg aatcgttatt cggaaaaatc aagaacatac
     3781 tcccgccgcc tgcactgctc tggatcttcg taatgatatt gtactctacg tccgtattcc
     3841 cgtttagatt acatccgtaa tcaccggtgt tgaaactggt tgtcgcagaa gtgatatcaa
     3901 tctcgcagat cagtttccag ctttttcccc cagactccaa tgaagtgagt ctagtatcca
     3961 gctcattcac cttcgtaaac aatgcatcaa aattggcgtt cacttgtgag gagtttacag
     4021 cggttcctga ggaaaatgca taaggctttg ctacagaaac agcccagcgg ctgagaggaa
     4081 tcagaaacag tatacatgtc atcattatga gtattttttt gagcatcttt ttcctttctt
     4141 cattccttct ttccatataa tacttttcaa aaatctcccg aatgtactgt tcattcataa
     4201 aattctcctt tttgtaccga tcccttgccc gtccgaagca ga
//
LOCUS       DAOQGJ010000082         4211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11398_length_4211_cov_3.498075, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000082 DAOQGJ010000000
VERSION     DAOQGJ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11398_length_4211_cov_3.498075"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..676)
                     /locus_tag="PK683_02080"
     CDS             complement(59..676)
                     /locus_tag="PK683_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07263.1"
                     /translation="MSRRFRERLTEKITEIESVSGAETVITVSENSGDYQYLNLCSGI
                     FFGLSAFTYTMFSPEFFPEEWIYFSSLIGFGLGYLFFLVKPLAAMFVSKEKLGRNTEI
                     YARACFQKGKIYETSSRQGILIYISMFERTSVLIGDRNASLKVPVNEWNLLREKFQKV
                     FHPISRKKTEENILLELSLFQDIMKKYIPESGEINEIPDGLEIFL"
     gene            complement(676..1596)
                     /locus_tag="PK683_02085"
     CDS             complement(676..1596)
                     /locus_tag="PK683_02085"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNH07264.1"
                     /translation="MKPDLKYSYFQGKIVPAEEAMVSIQTHALQYGTSVFGGIRGYYN
                     KSKDNIFVFRLKDHFRRLLNSVRIMQLQYENTPEILAEAAIELIRKNELKENVYLRPF
                     VYTSALQLSPRFHDVRADLAIYALRLNDYLDTQNGLKAMVSSWRRIDDNMIPTLSKAA
                     GGYVNSALAKSEAVQNGFDEAVFLDSRGYVSEGSAENIFIVSDGKLITPSLSSAILGG
                     ITRRTLIELCQTLKLEVIERQVTRSELYTADELFFCGTGVQVAWIKEVDRRVIGNGEI
                     GPITKKIQTEYFRIVTGENSDFSKWLSPVY"
     gene            complement(1598..2863)
                     /locus_tag="PK683_02090"
     CDS             complement(1598..2863)
                     /locus_tag="PK683_02090"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNH07265.1"
                     /translation="MSISKKTIRDIKLTGKKVLMRVDFNVPIKNGVIQDETRIAASLP
                     SIRYILEQGAESIVLMSHLGDPGKDMKKAKEKADKAGKPFNEEEYINGQHRMKPIGEC
                     LSRHLGKPVLTLNSCIGPEVESAAAGAKGGQVILLENTRFHKEETSESAEEREKLAKQ
                     LCKYGEIYVNDAFGTAHRAHASTETAAKYLPAVAGFLMEKELEFLQEKVVNSPAKPFV
                     AIVGGAKVSSKIAVIESLLDRTDKIIIGGGMAFTFFKAMGKQIGTSLCEEDMLETASK
                     ILAKAKANKVDFILQTDAVAASAFANDAKTVTVDADHIPADMMGLDIGPKSIELFKKA
                     LSDAKTVFWNGPVGVFEMDSFAKGTLEIANTLASLNAVTVIGGGDSVSAVNKAGVADK
                     MTHISTGGGASLELVEGKTLPGVAALNNK"
     gene            3166..>4211
                     /locus_tag="PK683_02095"
     CDS             3166..>4211
                     /locus_tag="PK683_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07266.1"
                     /translation="MLNIYINEQVIYWLAILILNLAIIGYLFMLKPKNTATSLLICAF
                     LSGCGLYVSAVVQTSTESLKLLSSLQRKIQLPFVILAFLFFIQFAYYFPGQIKEKKRE
                     RINALVLSVIAVLSGVFISLFSILFPALPELETYIVQLLILLAVILAFFSAVIRNSIL
                     FSRKKDSKSASALRSFLMPFSVIIPVIISEILFTAGFIPEKVESFFLSVGILSFNFLF
                     YLSFINNTSEQSSSFLTKIIGVTLMTVLIVWGNLGNFIAPYFRENYKNFNIVHRNSTL
                     KFVPNADRSYDISSVPFLMEKDFGRPLSFENHGKSSSVSYSFSFFGKEHSRIQAAPLP
                     ALFFHLDEVHLSYDL"
BASE COUNT         1081 a          937 c          832 g         1361 t
ORIGIN      
        1 agaaaggact gaaacagaga taatgaaaaa aaaatgataa tgtatgaaag aacagtcttc
       61 aaaggaaaat ctccagaccg tcaggaatct catttatttc tccggattcg ggtatatact
      121 ttttcattat atcctgaaac agactgagtt ccagcagaat gttttcttct gttttctttc
      181 tcgaaatggg atggaaaact ttctgaaatt tctctctcag cagattccat tcattcacgg
      241 gaactttaag agaagcattt ctgtctccaa taaggactga tgttctttca aacatggaaa
      301 tatagatgag aatcccctgt ctcgaagagg tctcatagat ttttcctttc tgaaagcagg
      361 ctctcgcata aatttccgtg ttccttccga gtttttcctt tgagacaaac attgcggcaa
      421 ggggtttcac caaaaaaaac aagtatccta ggccgaatcc tatgagcgat gagaagtaga
      481 tccactcttc agggaaaaat tcaggactga acatcgtata agtgaatgcg ctcagtccaa
      541 aaaaaatgcc ggaacaaaga ttcagatact gatagtctcc tgaattttcg gatacagtaa
      601 tcacagtctc cgcaccggag actgattcaa tttctgttat cttttcggtc agcctttctc
      661 tgaatctcct gctcatcagt acactggact cagccatttt gaaaagtcag aattttctcc
      721 tgtgactatc ctgaaatatt cagtctgaat ttttttagtg attggaccga tttcaccgtt
      781 tccaatgact ctcctgtcga cttctttgat ccaggcaacc tgaaccccag ttccgcagaa
      841 gaacagttca tctgcagtgt aaagctcgct tcttgtcacc tgtctttcga tcacttccag
      901 tttcagagtc tgacagagtt caatcagtgt ccttctcgta atgcctccga gaattgccga
      961 gcttaatgat ggtgtaatga gttttccatc agatacaatg aagatattct ctgcagatcc
     1021 ttcagaaacg tatcccctgc tgtccagaaa gaccgcctca tcaaatccgt tctgaactgc
     1081 ttccgatttt gccagagcag agtttacata tcccccggcg gccttggaaa gagtagggat
     1141 catattgtcg tctatccgtc tccagctgga caccattgcc ttcagaccgt tctgagtatc
     1201 cagatagtca ttcagacgga gagcatagat tgccagatcc gcccttacat catgaaaacg
     1261 cggggagagt tgaagagcgg atgtgtatac gaacggcctg agataaacat tctctttcag
     1321 ttcattcttt ctgatcagtt caatggctgc ttctgccagt atctcaggag tgttttcata
     1381 ctgcagctgc ataatcctga ctgaattcag aagccttctg aaatgatcct tcagtctgaa
     1441 gacaaatata ttatccttgg acttattgta ataaccgcgt attcccccga atacgcttgt
     1501 tccgtactga agggcatgag tctgtatgct gaccattgct tcttcagcgg gaacaatttt
     1561 tccttgaaaa taagaatact taagatcagg tttcatatca tttattgttc agtgcagcaa
     1621 ccccgggcag agtttttcct tctaccagtt caagtgaagc accgcctccg gtggaaatat
     1681 gagtcatttt gtctgcaact cctgccttgt tcaccgcgga tacggaatca ccgcctccga
     1741 ttacagtcac tgcattcaga gaagcaaggg tgtttgcaat ttccagagtc cccttggcaa
     1801 aactgtccat ttcaaatact ccgacaggac cgttccagaa tacagtcttc gcatcagaga
     1861 gagctttctt gaaaagttca attgatttgg gacctatatc gagtcccatc atatcggcag
     1921 gaatatgatc tgcatccact gtgactgtct ttgcatcatt tgcaaatgcg gaagctgcaa
     1981 ctgcatcggt ctgaagaatg aagtctacct tgttcgcttt tgccttggca agaattttgg
     2041 atgcagtttc cagcatatct tcttcacaga gagatgttcc tatctgtttt cccattgcct
     2101 tgaagaatgt gaatgccatt ccgcctccga taatgatctt gtcggttctg tccagaaggg
     2161 attctatgac tgcgattttg gaagagacct ttgcacctcc aacgatagcg acaaaaggtt
     2221 ttgccggaga gttgactact ttttcctgaa gaaattccag ttccttttcc atcagaaaac
     2281 ccgcaactgc aggaagatat tttgctgcag tttcagtgga agcgtgtgct ctgtgtgctg
     2341 ttccgaatgc atcatttaca tagatctcac cgtacttgca gagctgcttt gcaagtttct
     2401 ctctttcttc tgccgattcg gaagtttctt ccttatgaaa tcttgtattt tccagaagta
     2461 tgacttgacc gcctttggct ccagcagcag ctgattccac ttcagggcct atgcaggaat
     2521 tcagtgtaag aaccggtttc ccaaggtgtc tggaaagaca ctccccgatc ggcttcattc
     2581 tgtgctgacc gttgatgtat tcttcttcat tgaacggttt gccggcctta tctgcttttt
     2641 cctttgcctt tttcatatcc ttgcctggat cacccaggtg ggacataaga acgatcgatt
     2701 ctgcaccctg ttctaatata taacgaatgg aaggaagaga agccgcaatt ctggtttcat
     2761 cctgaatgac tccgtttttt attgggacgt tgaaatccac tctcatcagc accttttttc
     2821 cggtcagctt gatgtctctg attgttttct tgcttatgga catagttctc tcctctttag
     2881 tccaaagtaa attctgcagc tgaattgaaa agaagattcc gtttttaaag actgcatcag
     2941 tgtattctga ggttgaaaat cattggactc agtttggaat gagtgaatca taaaatagca
     3001 agtctgtgtt tcagcgatca agctctgcat cggtttcaca cagctgctcg acagagagtc
     3061 tttccgtgcg gctgcggcat aattcagttc aaattaaaaa aatgctttat ttcattgtga
     3121 ccggaacaga tataaggata gggaaggttt cccggcggga aaggtttgct gaacatatat
     3181 ataaacgagc aggtcattta ttggcttgcc attctgattt taaatctggc aattatcggc
     3241 tatctgttta tgctgaagcc taaaaatacg gccacttctc ttttgatctg cgcttttctt
     3301 tccggatgcg gtctttacgt ttctgcagtt gttcagactt ctactgaatc attgaaactg
     3361 ttaagttcac tgcagagaaa gattcagctt ccctttgtca tacttgcatt tcttttcttt
     3421 attcagtttg catattattt tcccgggcag atcaaagaaa aaaaaagaga aagaataaat
     3481 gctcttgtac tttctgtgat tgctgttttg tccggtgtat tcatttctct tttctctatt
     3541 ttgtttcctg cactgcctga gctggaaact tatattgtcc agctgctgat cctgcttgca
     3601 gtcatccttg cttttttcag tgcagtcatc cgcaattcaa ttctgttcag caggaaaaag
     3661 gattcaaagt ctgcctcggc actgagatcg tttctgatgc cgttttctgt aattattcct
     3721 gtaattattt cagagatact ttttactgcc ggtttcattc ccgaaaaggt ggaatcattc
     3781 tttctttctg tcggaatact gtccttcaat tttctgttct atctttcctt tatcaacaat
     3841 acatctgaac agtcctcctc gtttctgacg aaaattatag gtgtcactct gatgactgta
     3901 cttatagtct ggggaaatct tggaaatttt attgctcctt atttcagaga gaactataaa
     3961 aatttcaata tagttcatag aaacagcact cttaaatttg ttccaaatgc ggacagaagt
     4021 tatgacattt cctctgttcc attccttatg gaaaaggatt tcggtcggcc tctgagtttt
     4081 gaaaatcatg gaaaaagcag ttcagtctcc tattcatttt cctttttcgg aaaggagcac
     4141 tcaagaattc aggccgctcc gctccctgca cttttctttc atttggatga agtgcattta
     4201 agctatgatc t
//
LOCUS       DAOQGJ010000083         4194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11468_length_4194_cov_3.052187, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000083 DAOQGJ010000000
VERSION     DAOQGJ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11468_length_4194_cov_3.052187"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PK683_02100"
     CDS             <1..677
                     /locus_tag="PK683_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07267.1"
                     /translation="LLAKESPRAEDRIVNIGIAGSSKDDYKIGSVFLINKITDSASGK
                     SFYPETNFRHELKEERIVTFDRPARKNDLVDSQDGLADMEASGFYEAAGNYFHSHRIH
                     ILKVISDHMRGEKLTGAFVQGLMERSIQSLKILLARASKASVPDSDVLNQGDYKVFDN
                     VSNELNLSATQRFQVLDLIKGYKVKGGDDLNFIKGYVKQKKNTKQENKKIFEALKEKL
                     EDLTDD"
     gene            698..1117
                     /locus_tag="PK683_02105"
     CDS             698..1117
                     /locus_tag="PK683_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07268.1"
                     /translation="MKNFKDFNFKLMVIQELMYHREVLDPIFDLDEFAENFSDRKIDT
                     DTVSYEIIPEVKKHFENLDIPDELLDSVTELNADGGDDIYFQLCPHWDGEDDLFNVKS
                     ADDCARLKNLKRVTIFYDDDASILKKFAKFGVKADWV"
     gene            1172..2071
                     /locus_tag="PK683_02110"
     CDS             1172..2071
                     /locus_tag="PK683_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07269.1"
                     /translation="MKIILTLFLMLISFSSAVSEKVYVGIYVVNLGKFEVATGSFTAD
                     FYMTLKTEKEAEFPLDSFEFMNGRAVSSEKILEEPNLVQYRIMANLTNPVDLRKFPFD
                     DQILSIIIENKKYTSDKVKYVIVDRDSGLDEMIAFAGWNIRGWKAEERDHIYKVFNET
                     YSLASFNIQIEKIRWNAFFKTFLPVLIIIFLVNCSFIMDTDKIKDRLGICTSGLVASV
                     MFHLSISNQIPAVSYLTIADKFMLHTYIVLLMCVLLNVFMLQLIQVRNEDITQKIHRF
                     TKYGMFIVIPAIYMLFFAVNLTD"
     gene            2138..2413
                     /locus_tag="PK683_02115"
     CDS             2138..2413
                     /locus_tag="PK683_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNH07270.1"
                     /translation="MPIIVESDPEVKEDISKIYRHRVVLYNDDINSFDHVIECVMTIC
                     GKNEEEAFKITLEAHEKGKSVCFDGSIEACETVAEKMGIENLTVSLH"
     gene            2456..2893
                     /locus_tag="PK683_02120"
     CDS             2456..2893
                     /locus_tag="PK683_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNH07271.1"
                     /translation="MKTIPSLLLFLFFINCSGTRPQDIGVRQGRLKPCPEKPNCVNSQ
                     TDIQDRIHFIEPVSYTLPLLDEQKRLKETVLGLPRTSLIKSETGYMHFEFTSLLMRYK
                     DDVEFYLDDSVKLIHIRSASRLGTSDMGVNRKRTEEIRTLLKK"
     gene            2918..>4194
                     /locus_tag="PK683_02125"
     CDS             2918..>4194
                     /locus_tag="PK683_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNH07272.1"
                     /translation="MEINFSTKEYTSGDSFQETEYRFNGSAEDGWVIERNRSHYLTLD
                     KGYRLLKTKVCGVCSTDLDRRFLPFPLPQIIGHEVIAEDLESGEKFAIEINDTDAAKG
                     KKPEDIFCTSGIPTHQPDRMVLGIDRLPGGFGPYILSPVNAAVPIGNVPEMTAVLTEP
                     FAAALQAVIASPPANGTEVAVLGPRRLGSLLIAALHAYRHSSGKNFKIAALARHSHLL
                     ELASELGADILYDLRTAPKKELQRRFSIVYDTTANPEGFLTAMEYSSAEVHLKSTNGQ
                     TVSGLKKMTEAVVDELSILPFSEENLHFSWDSPKVRNERIYIADSKFEHPSLKSFRTY
                     RKTPTEAFDLIRSDEFAGRLPRFDLCIASSLQDIDSVIRPKIDSEESLLRPRGAVLFT
                     GKTDGNPLLDFLMKGGRIRTSRCGDFHLALKLMS"
BASE COUNT         1291 a          775 c          943 g         1185 t
ORIGIN      
        1 atcttctggc aaaagaatct cctcgtgcag aggacaggat cgtcaatatc gggattgccg
       61 gctcatcaaa ggatgactac aagatcggat cagtgttcct gatcaataag atcaccgaca
      121 gtgcttcagg aaaatccttt tatcctgaaa caaatttccg tcatgaactg aaagaggaaa
      181 gaatcgtcac ttttgacagg ccagcaagaa aaaatgacct ggtggacagt caggatggtc
      241 ttgcggatat ggaagcttcc gggttttacg aggctgcagg aaattatttt cattcccaca
      301 ggatacatat tctgaaggtc atctccgacc atatgagagg agaaaagctt acaggagcat
      361 tcgttcaggg actgatggaa agaagcattc aatcgttgaa aatcctgctt gcccgcgcat
      421 caaaggcaag tgtccctgat tcagatgtgc tcaatcaggg agattataaa gtatttgaca
      481 atgtaagcaa tgaactgaac ttgagcgcta ctcagagatt tcaggtgctt gacctgatca
      541 aaggctacaa agtcaagggc ggagatgatc tgaacttcat caagggatat gtgaagcaga
      601 agaaaaacac gaagcaggaa aacaagaaaa tctttgaagc attgaaggaa aaactcgaag
      661 atctgactga tgactgaagc tttgacagga actgacaatg aaaaatttta aggatttcaa
      721 ttttaagctg atggtcattc aggaactgat gtaccatcgc gaagttcttg atccgatttt
      781 tgatctggat gaatttgctg aaaatttttc agacagaaaa atagatacag atactgtcag
      841 ctatgaaatc attcctgaag tcaaaaagca ttttgaaaat cttgatattc ctgacgaact
      901 tctggattct gtcacagaac tgaatgcaga cgggggagat gatatttatt tccagctttg
      961 tcctcattgg gacggagagg atgatctctt caatgtaaaa tctgcagatg actgtgccag
     1021 actgaaaaac ttgaaaaggg tcacaatctt ttacgatgat gacgcttcca tactcaaaaa
     1081 atttgcaaaa ttcggagtaa aggcagactg ggtctgacga atgcagatgg agcgtttttt
     1141 aagagattaa tctcagttct tctgtaatga tatgaaaatt attttgactc tttttttaat
     1201 gctcatttcc ttttcatctg ctgtctcgga aaaggtgtat gtaggaatct atgtcgtaaa
     1261 cctcggtaaa tttgaagttg cgacaggcag ttttactgct gatttttaca tgactctgaa
     1321 gacagagaaa gaagctgaat ttcctctcga cagttttgag tttatgaacg gcagagctgt
     1381 ttcctcggag aaaatactgg aagaacctaa tttggtccag tatcgaatca tggcaaattt
     1441 gactaatccg gtcgatctga gaaagttccc ctttgatgac cagattctga gcattatcat
     1501 tgaaaataaa aaatatactt ccgacaaggt aaagtatgtc attgtggaca gggattccgg
     1561 gcttgatgag atgattgcct ttgcaggctg gaatataagg ggctggaagg ctgaggaaag
     1621 agatcatata tataaagtat tcaatgaaac ctattcactg gcatcattca atattcaaat
     1681 tgaaaaaatc cgctggaatg cattcttcaa gacatttctt cctgtgctca ttattatttt
     1741 tctggtaaac tgcagtttca ttatggatac tgacaagata aaggatagac ttgggatatg
     1801 cacatccggt cttgttgctt ccgttatgtt tcatctttcc atttccaatc agatcccggc
     1861 agtaagttat cttacaattg cagataaatt tatgcttcat acttatattg ttcttctgat
     1921 gtgcgttctt ctgaatgttt ttatgcttca gctgatacag gtcagaaacg aagacatcac
     1981 tcagaaaatc cacaggttta caaaatacgg aatgtttata gtgattcctg caatttatat
     2041 gcttttcttt gctgtaaatc tgacggattg aatctgtccc gattttccgg aattcaggtt
     2101 tacaaattcg gtaaaagatt gagaatggaa gataaagatg cctataattg ttgaatccga
     2161 tcctgaagta aaagaagaca tttcgaaaat atacagacac agggttgtgc tttacaatga
     2221 cgatatcaat tcattcgacc atgtgattga gtgtgtgatg accatatgcg gaaaaaatga
     2281 ggaagaggct tttaaaatta cgcttgaggc acatgagaaa ggcaaatctg tatgctttga
     2341 cggaagcata gaggcctgtg agacagttgc cgaaaagatg ggaattgaaa atctgacagt
     2401 gagtcttcac tgattttcac ttgtaagtat tttccaaaaa aattatgatg tttctatgaa
     2461 aacgatcccg agccttcttt tgttcctttt ttttataaac tgttccggaa cgagacccca
     2521 ggatatcggt gtaagacagg gcagactgaa accttgtccg gaaaagccga actgtgtaaa
     2581 ttctcagact gatattcagg acagaattca ttttattgaa cccgtcagct atacactgcc
     2641 gcttcttgat gaacagaaga gacttaagga aactgttctt gggcttccta gaacaagtct
     2701 gataaaatcg gaaacgggtt atatgcattt tgaatttaca tccctgctga tgcggtacaa
     2761 ggatgatgta gagttttatc tggatgattc agtgaagctg attcatatac ggtctgcctc
     2821 aaggctcggt acttcggata tgggagtcaa cagaaaaaga acagaagaga tcaggactct
     2881 tctgaaaaaa taaagagcag accggatcgg agaatggatg gaaattaatt tcagtacaaa
     2941 ggaatatact tcaggggaca gttttcagga gacggaatac agattcaatg gaagtgctga
     3001 agacggctgg gtcattgaaa ggaacaggag tcattatctt acccttgaca aaggatacag
     3061 acttctgaaa acgaaagtct gcggagtctg ttcaactgat ctggacagac ggttccttcc
     3121 tttcccgctt cctcaaatca tcggtcacga agtcatcgca gaagatttgg aaagcggcga
     3181 aaaatttgcc atagaaatca atgatactga tgctgcaaaa ggaaaaaagc cggaagatat
     3241 attctgcact tcaggaattc caactcacca gcctgacaga atggttctgg gtatcgaccg
     3301 gctccccgga ggatttggac cttatatact ttcacctgta aatgctgctg ttccgatagg
     3361 gaatgtgccg gaaatgactg cagtcctgac tgaaccgttt gcggcagcac ttcaggctgt
     3421 aattgcttct ccgcctgcaa atggaactga ggttgcggtg cttggtccaa gaagactcgg
     3481 atctcttctg attgctgcct tgcatgcata cagacacagt tcaggaaaga attttaaaat
     3541 tgctgcactg gccaggcatt ctcatcttct ggaacttgct tcagagctgg gagcggatat
     3601 tctgtatgat ctgagaacag ctccgaagaa agaactgcaa agaaggtttt cgatcgtata
     3661 tgatacaaca gcaaaccctg agggttttct gactgcaatg gaatactctt cagctgaggt
     3721 tcatctgaaa tctacaaacg gacagacagt aagcggactt aagaaaatga cagaggcagt
     3781 agtggatgag ctgagcattc ttcctttttc cgaggaaaat ctgcattttt catgggacag
     3841 tcctaaggtc cgcaatgaaa gaatttatat tgctgattct aagtttgagc atccttctct
     3901 gaaatctttc agaacataca gaaaaacacc gacggaagca tttgacctga tacgttctga
     3961 tgaatttgca ggacggcttc cgcgatttga cctttgtatt gcatcttcat tgcaggatat
     4021 tgattctgtg atacgtccta aaattgattc agaggaatct cttcttcgtc caaggggagc
     4081 tgtccttttt acaggaaaaa cagacgggaa tccgcttctg gactttctga tgaaaggcgg
     4141 aagaatcagg acatccagat gcggagattt tcatctggca ctgaaactta tgtc
//
LOCUS       DAOQGJ010000084         4193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11472_length_4193_cov_3.204447, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000084 DAOQGJ010000000
VERSION     DAOQGJ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11472_length_4193_cov_3.204447"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PK683_02130"
     CDS             <1..768
                     /locus_tag="PK683_02130"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07273.1"
                     /translation="AAMKAREEKEKLQQLKHDLSIASKIQTYSLPKLPERIGDLEIGS
                     AYFASKEIGGDFYDFIYHKEGLFTVLIADVAGKGIPAALIMEYSKTLLSSEMQRAVSP
                     SVALLKTHRQLLEKPEKIPHVEVMAVRIDSINRRLSIGSAGHNRQIFYNSFTDSIELL
                     RAKGIPLGAKIEIDSFQETIRDYRRGDMLFLYTDGITECRNEKDEMYGEERLFLFVLR
                     EKENSTEIMRQRLIEDLEKFRGRRDFEDDYSFLIIKL"
     gene            complement(769..1401)
                     /locus_tag="PK683_02135"
     CDS             complement(769..1401)
                     /locus_tag="PK683_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNH07274.1"
                     /translation="MKQIISLFFILFSCIEISAWELKRDSEGIQVYTRPIEGSQLKEF
                     KAVTVYKGSLKSITAVMRDPNAACSWFPDCREFRSLKVNSPFSSVEYIRIKAPFPVSD
                     RDNITQTVLSQDKKTKAVKISITSLPKYLPETAGVVRVTKVQGYWLFTPKSENETEIV
                     YQVHAEPGGSLPDMIANSFVTESPFKALKNLKNKAKEEKYQNADMSKIIE"
     gene            complement(1398..2912)
                     /locus_tag="PK683_02140"
     CDS             complement(1398..2912)
                     /locus_tag="PK683_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07275.1"
                     /translation="MNKELLLSKISEIRDETVPCLDFAREKAAALGKTYSALRAEDLI
                     YKELLRIISYIDFNEKISPNAVLLMTEVLNRLFRDISWNEESFRLNLEEYKKKNPAYG
                     TVFNTDEFEIPEVLNAAKEYDAQFTSDKFDRISLFFFQILNLIIKADEKLTVFEEKFL
                     LRYNAGISKMKFKNTDIGTEFAAQLNSIYNDFFGYSSNLKQDIQKPDASVKKGAEPKK
                     PEYAKIEEKPPEKLEDLLAELNGLIGLKKVKEEIDNLINVIKIEKIRKEKGLSVPDKS
                     LHLVFTGNPGTGKTTVARILSRIYKALGVLSKGHLVETDRTGLVAGFVGQTAIKTTEL
                     CNSALDGVLFVDEAYALAEGGENDFGREAINTLLKFMEDNRNRIIVTVAGYTHNMEEF
                     IAKNPGLKSRFNTYIHFDDYTPDELMMIFSKTCKSMKLHLTEDAEKKLKEIFQKAYDE
                     RDDKFGNGRFARNLFEKVYRRQANRLVLLTEITEEALSEIRAEDLEDREKGKTE"
     gene            complement(2916..3392)
                     /locus_tag="PK683_02145"
     CDS             complement(2916..3392)
                     /locus_tag="PK683_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07276.1"
                     /translation="MIGFIKKLFGIGGDSERDKIDEVADRVLRFADRFRNADDEDGAE
                     IAEQYAEEIRKVRTLQEAYAIESEFMQKIKDRLPFGKKGTASHFGGVTHHHYDHDHFD
                     TSDDWVETTSAVSGIYGNGNTPSTDSSETSEGDGGDFSGAGSSTEFDSGETFSDNS"
     gene            complement(3389..>4193)
                     /locus_tag="PK683_02150"
     CDS             complement(3389..>4193)
                     /locus_tag="PK683_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07277.1"
                     /translation="LITMEMAEGTSSLENDKYTYTIQGKDILWQKERLLNLGMEKLPR
                     ECRYVVWLDCDVIFSDEDWAAEAEKQLEKYTVIQPFETAVRLPKDHSFFQGEGESWNS
                     FAKVFREEPNLFLFGEFDAHGHSGFAWGARRDLFQKHRLYDCCILGSGDHMMAHAFAG
                     DWQSYCIRRIFRNNKTHFSHFQAWAKRIYSEIKASIGTCSSALYHLWHGDAENRKYVS
                     RNWKLAEFQFDPDKDVKIGKSGLFEWNSDKIELHKYCENYFLERKEDGI"
BASE COUNT         1042 a          962 c          845 g         1344 t
ORIGIN      
        1 gccgcaatga aggcgcgtga agagaaggaa aaacttcagc agctgaagca tgatctgagt
       61 attgcaagca agatccagac atattccctt cccaagcttc cagaaaggat aggcgatctg
      121 gagattggtt ccgcttattt tgcctccaag gaaatcgggg gagattttta tgatttcata
      181 tatcataagg aaggactgtt cactgttctg attgcagatg ttgccgggaa ggggattcct
      241 gctgctttaa ttatggaata ttccaagact cttctttcca gcgagatgca gagagctgtt
      301 tccccgtctg tcgctcttct gaaaacacac aggcagcttc tggaaaaacc ggaaaaaatt
      361 cctcatgtgg aagtcatggc tgtgagaata gacagtataa acagaaggct cagcatcggc
      421 agtgcaggcc ataacaggca gattttctac aattcattca ctgattcaat tgagcttctt
      481 cgcgcaaagg ggattcctct cggtgcaaag attgaaatcg attccttcca ggagacaatc
      541 agggattaca gaagaggtga tatgctgttc ctctacactg acgggatcac tgaatgcaga
      601 aatgaaaagg atgaaatgta cggagaagaa agactgtttc ttttcgtact tagggagaaa
      661 gaaaacagca cagaaattat gagacagaga cttatcgagg atctggaaaa attcagagga
      721 agaagagact tcgaagacga ctattccttt ctcatcatta agctgtaatt attcaataat
      781 tttgctcata tctgcgttct gatacttctc ttccttcgcc ttgtttttca gattcttcag
      841 cgccttaaaa ggtgattcag ttacaaaact gttggcgatc atatccggaa gcgatccgcc
      901 aggttctgcg tgcacctgat atacaatctc tgtttcattt tccgatttag gagtgaaaag
      961 ccagtaaccc tgcacctttg taactcttac aactcctgcc gtttcaggaa gatatttcgg
     1021 cagggacgtg atgctgattt tcaccgcctt tgtttttttg tcctgactca gaactgtctg
     1081 agtgatattg tctctgtcgc ttacaggaaa aggagccttg attcttatgt actcaactga
     1141 gctgaacgga gagttgacct tcaaagatct gaattcccga cagtcgggaa accaggagca
     1201 ggctgcattc gggtctctca tcactgccgt tatgcttttc agtgaccctt tgtatacagt
     1261 cacggctttg aattctttga gctgagatcc ttcaatcggc ctggtgtata cctgaatccc
     1321 ttcgctgtct ctcttcagtt cccatgcaga aatttcaatg catgaaaata aaataaaaaa
     1381 taatgatatg atctgtttca ttcagtcttc cctttttccc tgtcttccag gtcttctgcc
     1441 cgtatctctg aaagagcttc ttcagtaatc tcggtcagga gcaccagtct gtttgcctgt
     1501 ctgcggtata ccttttcgaa caggtttctc gcaaaacgcc cgtttccgaa tttgtcgtct
     1561 ctttcgtcgt atgctttctg gaatatttcc ttgagctttt tttccgcatc ctctgtaaga
     1621 tgaagcttca tgcttttgca ggttttgctg aagatcatca tcagttcatc aggagtatag
     1681 tcatcaaaat gaatgtatgt gttgaatctg gatttcagtc caggattttt tgcaatgaac
     1741 tcttccatat tgtgagtgta gcctgcgacg gtgacgatga ttctgtttct gttgtcttcc
     1801 atgaatttca gaagagtatt gattgcttct cttccgaaat cattctcacc gccttctgca
     1861 agtgcatacg cctcatccac aaaaagaact ccgtccagcg cagaattgca gagttcggtt
     1921 gtttttatcg ctgtctggcc cacgaatcct gccactagcc ctgttctgtc agtttccacc
     1981 agatgtcctt tggacagcac acccagtgct ttgtaaatcc tcgaaaggat tctcgccaca
     2041 gttgtctttc ctgttcctgg gtttcctgta aaaaccagat gaagactttt atccggaaca
     2101 gaaagacctt tttcctttct gattttttcg atcttgatta cattgatcag gttgtcaatt
     2161 tcttccttga cttttttcag accgatgagt ccattcagtt cagccagaag atcttccagt
     2221 ttttcaggcg gcttctcttc aatttttgca tattcgggtt ttttcggttc tgctcccttt
     2281 tttacagatg catcaggctt ctgtatatcc tgcttcagat tggaggaata tccgaagaaa
     2341 tcattgtata tgctgttcag ctgtgctgca aactcagttc cgatatctgt attcttgaat
     2401 ttcatcttgg atatgcctgc attgtatctg agaagaaatt tctcttcaaa tacagtcagt
     2461 ttttcatctg ccttgatgat cagattcagt atctgaaaaa aaaatagact gattctgtcg
     2521 aacttgtcgg atgtgaactg tgcgtcatat tcctttgccg cattcagcac ttccgggatt
     2581 tcgaattcat cagtattgaa tacggttccg tatgcaggat tctttttttt gtattcttca
     2641 agattcagcc ggaaactctc ttcattccag gagatgtccc tgaaaagtct gttcagcact
     2701 tcggtcatca gaaggacggc gttgggagat attttttcat taaaatctat gtatgaaatg
     2761 attctcagga gttctttgta aatcaggtcc tccgcacgga gagcactgta tgtttttccg
     2821 agagcagcag ccttttctct cgcaaagtcc agacagggga ctgtctcatc tctgatttca
     2881 gatattttgg aaagaagaag ttccttgttc atatctcagc tgttgtccga aaatgtttca
     2941 ccgctgtcaa attcagtgct gcttcctgcc ccgctgaaat ctcctccatc tccttctgag
     3001 gtttcgctcg aatcggtgga aggggtgttc ccatttccgt atattccgga gactgcagaa
     3061 gttgtttcaa cccagtcgtc ggaagtatcg aaatgatcat ggtcataatg atgatgagtc
     3121 acaccgccga aatgagaagc tgttcctttt tttccgaaag ggagacggtc cttgattttc
     3181 tgcataaatt cagattcaat tgcatatgct tcctgaagcg ttctgacttt tctgatttct
     3241 tctgcatact gctccgcaat ctcagcacca tcttcgtcat ccgcgtttct gaatctgtct
     3301 gcaaaacgaa gaaccctgtc cgcaacttca tcaattttgt ctctttctga atctccgccg
     3361 attccgaaaa gtttctttat aaagcctatc atattccgtc ctctttccgt tctaaaaaat
     3421 aattttcaca gtacttgtgc aattcaattt tatctgaatt ccattcaaaa aggccggatt
     3481 ttccgatttt tacatcttta tcaggatcaa attgaaattc ggccagcttc cagtttctgc
     3541 tcacatattt tctgttttcc gcatcaccgt gccagagatg atacaaagcc gaactgcagg
     3601 tgcctatgct tgccttgatc tccgaataga ttcttttggc ccaggcctgg aaatgactga
     3661 aatgagtctt attgtttctg aatatccttc tgatgcagta agactgccag tctcctgcaa
     3721 acgcatgagc catcatatga tctccgcttc ccagaatgca gcagtcatac agtctgtgtt
     3781 tttgaaacag atctcttctg gcaccccagg caaatccgga atgtccgtga gcatcaaatt
     3841 cgccgaacag aaaaagattc ggctcctcgc gaaagacttt tgcaaaactg ttccagcttt
     3901 ctccttctcc ctgaaaaaaa gaatgatcct tcggaagcct gactgcagtt tcaaacggct
     3961 gaatgaccgt atatttttcc agctgttttt ctgcctctgc cgcccagtcc tcatcagaga
     4021 aaatgacatc gcagtcaagc catacgacat atctgcattc cctcggaagt ttttccattc
     4081 ccagattcag aagtctttcc ttctgccata gaatatcttt tccctgtata gtatatgtgt
     4141 atttatcatt ttcgagactg gacgtgcctt cagccatttc cattgtgatg agg
//
LOCUS       DAOQGJ010000085         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11533_length_4180_cov_3.035152, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000085 DAOQGJ010000000
VERSION     DAOQGJ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11533_length_4180_cov_3.035152"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="PK683_02155"
     CDS             <1..1552
                     /locus_tag="PK683_02155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07278.1"
                     /translation="LQKYIRLELGDGIVGWVAKEGKPVLIENAYEDPRFDSSWDKKTG
                     FVTTSLICVPLFQKNRLIGTLEVLNKTKDRIFDSFDLELLGYLADMAAIAIENAALNE
                     NLRKRIMELSLIYDFDRQISANTNIQELGEWLLDNCLRGLEAKSGSILLWNDAGGFLS
                     VLQSKGIPAEYINSIKIYPGEGVAGWVAFHKTPLLIQNIETDPRFRSSERLNYENASL
                     ISVPLLYQNKLIGILNINNKTDGYAFTKNDLALAVTVSERLAMAVSSSRIFHDLNESA
                     QENLRARRLMEKIIPSVIPTVPGLRLSVNYHPYKDIGGDFFNFFPMDETHFGILLADV
                     SGHGLSSALLSVMVNTMIQTFEKTVLKSPSSFLIQLNNSLAGKMGGYFLTAFYCVIDL
                     EKGEISYAKGGHPSPVLYRDMERKSMYLNSAGKIIGIIPNVLYEERRISFQKNDVLIV
                     FTDGIVETVNDRTGKTYGADRLSELLNESGIPVFSELNQRIIEDAFGYTDTRQFSDDV
                     TLITVKRE"
     gene            1789..3072
                     /locus_tag="PK683_02160"
     CDS             1789..3072
                     /locus_tag="PK683_02160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD family DEAD/DEAH box helicase"
                     /protein_id="HNH07279.1"
                     /translation="MNYSEEQRQVIFEDSDRVQVVAGAGSGKTFTMVSLICRLLEEGR
                     EKPSEILVITFSRKAAEEISERIERRVGKTDVRVHTFHAYCLQVIRKFHPEYSRNLPE
                     IISDAERDSFMKNFFHSHRFRVGGIPFEFLLSEQDRILEKYFPDLDSLLRVSFSEYKI
                     QNARLEFSDLVKIFLDGMSLKEDWAMNAASEIRRVIVDEFQDTDPEQMEWLRLLNPEK
                     LTVVGDDWQAIYGFRGASTKPFLEFSKVFSPCRVLFLSNNYRSLSDIIRTSALPISMN
                     RNNIQKSAVPVREGTGTVQKYFIREETDWNLLKEHMNGKEFRVLCRTNFRKKTLSKYG
                     FAEDALLTVHASKGLEFHSVVLDLTSGWSSDSEISEEQKEEERRILYVALSRAKDELI
                     IAGKYRPDKKEKKFLETEFFQYFKGLDELRLFPDA"
     gene            complement(3055..3867)
                     /locus_tag="PK683_02165"
     CDS             complement(3055..3867)
                     /locus_tag="PK683_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07280.1"
                     /translation="MAEQGSVLMSLSKEEKHRILQKIRSEYYSMSKESPEIFKILPFE
                     ERYTQVLKHRGNLEEFFFAEMQHIREMKKAYLARKEKRKILEESPIDRLIEEQQKSML
                     KYPGILFHPSAKKEIQHFFGAVSGFVEMEIPVLEKIFKGTPEMKELQEQTVFLERIGY
                     RRQGVLPPRIQDHIKLIHTENIDQKIIEKHIQDIVKDGCIALSNIVKSLSRFSEEDLF
                     SKLVLKFSENEPENLRVIYSDISYKWALDKIQSRANEIIRDFRMHSLLSIRK"
     gene            complement(3843..>4180)
                     /locus_tag="PK683_02170"
     CDS             complement(3843..>4180)
                     /locus_tag="PK683_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07281.1"
                     /translation="TYPRPAATYSIGYSKTKKEKINSLKDEKLQMEYILSNEMQNMFY
                     TILKNMEEIFLEIREKLKSFSGEVHTKAMNPSQKRAFEDAKQSAEFTYSEVSSLRTYI
                     EKWLSRDLY"
BASE COUNT         1200 a          800 c          935 g         1245 t
ORIGIN      
        1 gctgcagaaa tacatccgtc tggaactggg ggatgggatt gtcggctggg tagccaaaga
       61 aggaaagccg gtactgattg aaaatgccta tgaagatcct cggttcgatt ccagctggga
      121 caaaaaaaca ggatttgtaa ctacctcact gatctgcgtc cctctttttc aaaagaacag
      181 actgatcgga actctggaag ttctgaacaa gacaaaagac agaatctttg attccttcga
      241 tctggagctt ctgggctatc tggcagatat ggctgcgatt gccattgaaa atgcagcact
      301 gaatgaaaac ctccgcaaga gaatcatgga gctctctctg atctacgatt ttgacagaca
      361 gatcagtgca aatacgaaca tacaggaact tggagaatgg cttctggaca actgtcttcg
      421 cggactggaa gccaagtcgg gttctattct tctatggaat gatgcgggag gatttctttc
      481 cgtgcttcag tcgaagggaa tacctgcaga atatataaat tccattaaaa tatatcccgg
      541 tgaaggtgtt gcaggctggg tcgcttttca caagactcct ctcctcattc agaacattga
      601 aactgatccg agattccgtt catccgaaag gctgaactat gagaatgcat cacttatctc
      661 agttcctctg ctgtaccaga acaagctcat cggaattctg aacatcaaca acaagacaga
      721 cggatacgca tttaccaaga atgatcttgc actggcagtg actgtatcgg aaagacttgc
      781 aatggctgtg agcagcagca gaatttttca tgacctgaat gaatctgctc aggaaaatct
      841 gagggcgaga aggctgatgg aaaaaatcat tccatccgtg attccgaccg tccctggact
      901 gagactttcc gtcaattatc atccctacaa ggacataggc ggagattttt ttaatttttt
      961 tcctatggat gaaacgcatt ttggaatcct tcttgccgat gtttccggtc acggcctttc
     1021 aagtgctctt ctttccgtga tggtaaatac aatgatccag acatttgaaa aaactgtact
     1081 gaaaagtcct tccagttttc tcattcagct gaacaattct ctggcaggta aaatgggagg
     1141 ctatttttta actgcatttt actgcgtgat tgatcttgag aagggagaga tttcctatgc
     1201 aaagggcggt catccaagtc ccgtacttta cagagatatg gaaagaaaat caatgtatct
     1261 caattctgca ggaaaaatca tcggaatcat tcctaatgtt ctgtacgaag aaaggagaat
     1321 atcctttcag aaaaatgatg tgctgattgt attcacagac gggatagtgg aaacagtcaa
     1381 tgacaggaca ggcaagacat acggagccga caggctttca gaactcctga atgaatcagg
     1441 aattcctgta ttctctgaac tgaatcagag aatcatagaa gatgcttttg gttacactga
     1501 taccaggcag ttttctgacg atgtgactct cattacagtc aaaagggagt aggtttctga
     1561 gcaggatcaa aaggtaaaag tctgattctc ggttccggat aatttcagtt taatctatca
     1621 ttattttttg aatattttat actgattttt ttactgagca gcctctgcag gacattggtc
     1681 tctgcatatc tatcctcgaa agtctttcat ctttaaaata aaaaatcagg aaatacgaat
     1741 tttctgcgtc ctctgtcaaa aaaaatacag gtagttcagg aggatttaat gaattacagc
     1801 gaagaacaga ggcaggtcat ctttgaagat tcagacaggg ttcaggttgt ggcaggtgca
     1861 gggtcaggaa agacattcac aatggtcagc ctgatctgca gactcctgga agagggaaga
     1921 gaaaaacctt ctgaaatact tgtgatcaca ttttccagga aggctgcaga agagatatcg
     1981 gaaagaatag agcgacgtgt cggaaagact gacgtgagag tgcatacatt tcatgcttac
     2041 tgtcttcagg tcattcgaaa gtttcatcct gagtacagcc ggaatcttcc agaaatcatt
     2101 tcggatgcgg agagggacag ttttatgaag aatttttttc attcccacag attcagagtg
     2161 gggggaatcc cttttgaatt tctgctcagt gaacaggaca ggatactgga gaaatatttt
     2221 cctgacctgg attctcttct cagggtgagt ttttcagagt ataagattca gaatgcccga
     2281 ctggaatttt cagatcttgt aaagattttt ctggatggga tgtctttgaa ggaagactgg
     2341 gcaatgaatg cagcttctga aatcaggaga gtcattgtag atgaatttca ggatacagat
     2401 cctgaacaga tggaatggct gagacttctg aatcctgaaa agctcactgt ggtcggagac
     2461 gactggcagg ccatttacgg tttccgaggg gcgagcacaa agccctttct ggaattttcg
     2521 aaggtctttt ctccctgcag ggttcttttt ctttcaaaca actacagatc gctttcagat
     2581 atcatcagaa catctgctct acctatttcc atgaacagaa acaatattca gaagagtgct
     2641 gttcctgtcc gggaaggaac tggaacagtt cagaagtatt ttataaggga agagactgac
     2701 tggaatcttc tgaaagaaca tatgaatgga aaagaattca gagttttgtg cagaacaaat
     2761 ttcagaaaga agacactgag taaatacggt tttgccgaag atgctctact gacggtacat
     2821 gcatccaagg gtctggagtt ccattcagta gtgctggatc tgacgtcggg ctggtcgagt
     2881 gattcggaaa tttcagagga acagaaggaa gaagaaagga gaattctgta tgttgcattg
     2941 agccgggcaa aggatgaact gattatagcc ggaaaataca gaccggataa aaaagaaaaa
     3001 aagtttctgg aaacagaatt ctttcagtat tttaaaggac tggatgaact tcggttattt
     3061 cctgatgctt aaaagagagt gcattctgaa atccctgatg atttcatttg ccctggactg
     3121 tatcttgtcg agagcccatt tataggaaat atccgaataa atgactctca gattttcagg
     3181 ttcgttttct gaaaatttca gaaccagttt tgaaaacagg tcttcctcgg aaaatcggga
     3241 aaggcttttt acaatattgg aaagggcaat gcatccgtcc tttacgatat cctgaatatg
     3301 cttttctatg attttctgat caatattttc agtatgtatc agttttatgt gatcctgtat
     3361 tctcggggga agaaccccct gacgtctgta gccgattctt tcgagaaaca ctgtctgttc
     3421 ctgaagctcc ttcatttcag gagtaccctt gaatattttt tcgaggacgg ggatttccat
     3481 ttccacaaac cctgagacag caccgaaaaa atgctgaatc tcttttttgg ctgaggggtg
     3541 gaacagtatg ccgggatatt tcagcataga tttctgctgc tcttcaatca gtctgtcgat
     3601 gggactttct tcgagaattt ttcttttttc ctttctggca agataggcct ttttcatttc
     3661 cctgatatgc tgcatctctg caaaaaagaa ctcttccaga tttcctctgt gcttcagtac
     3721 ctgagtatat ctttcttcga aggggagaat tttgaaaatt tccgggcttt ccttggacat
     3781 agaatagtat tcagatctta tcttctgaag tattctgtgt ttttcttcct tggaaaggga
     3841 catcagtaca gatccctgct cagccatttt tcaatataag ttctgagaga actgacttca
     3901 ctgtaagtaa attctgccga ctgttttgca tcttcgaatg ctcttttctg agaaggattc
     3961 attgccttgg tatgcacttc accggaaaaa gatttgagtt tttctctaat ttcgaggaaa
     4021 atctcttcca tattcttcag aatggtataa aacatattct gcatttcatt ggaaagaata
     4081 tattccatct gcagtttttc gtctttcagt gaattgattt tttccttttt tgtcttcgaa
     4141 tagcctatgg aatatgttgc tgcgggtctg ggatatgtag
//
LOCUS       DAOQGJ010000086         4166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11590_length_4166_cov_3.512284, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000086 DAOQGJ010000000
VERSION     DAOQGJ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11590_length_4166_cov_3.512284"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK683_02175"
     CDS             <1..250
                     /locus_tag="PK683_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNH07282.1"
                     /translation="LQKAIPVRVHVSAYDRPKIYMEIVGAISKTDTNILEAGATATGE
                     GTVLAKFLLEIEHLDQLKEILDNIKSIPNIITVERTKT"
     gene            247..1116
                     /gene="rfbA"
                     /locus_tag="PK683_02180"
     CDS             247..1116
                     /gene="rfbA"
                     /locus_tag="PK683_02180"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNH07283.1"
                     /translation="MKGIILAGGSGTRLYPVTRVVSKQLMPVYDKPMIYYPLSVLMLT
                     GIREILIISTPGDSPLFEKLFGDGSSLGIRIEYAVQPAPEGLAQAFLIGKKFLNGEPS
                     CLILGDNIFYGHGMIESLSDSALLQNGGIIYGYYVNDPERYGVVEFDSKGNVLSLEEK
                     PERPKSNYAVPGIYFYDGKASEYAEQLKPSKRGELEITDLNRMYLEKKSLKVNLLGRG
                     VAWLDTGTHESLSQAGQFIEIIEKRQGLKVGCIEEIAYRKKYITKEQLLVHAESMIKN
                     EYGQYLKRIADEI"
     gene            1106..1654
                     /gene="rfbC"
                     /locus_tag="PK683_02185"
     CDS             1106..1654
                     /gene="rfbC"
                     /locus_tag="PK683_02185"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNH07284.1"
                     /translation="MKFNDTEFADLKVIEPTVFKDSRGWFFESYSEKKFAENGIKSEF
                     IQDNHALNSDPNTVRGLHFQKPPFEQAKLVRVVKGAIFDVAVDLRRNSPTFRKWFGIE
                     LTSFNRTMLFVPRGFAHGYLVLKPDTEVLYKVDNLYSPESEGGIIWNDPELSIDWMVN
                     SPILSPKDAVLPPLSECLNIFL"
     gene            1945..2439
                     /locus_tag="PK683_02190"
     CDS             1945..2439
                     /locus_tag="PK683_02190"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNH07285.1"
                     /translation="MRILIDGFNLIYKFPDLEGLMYESRLNLARQGLLQILHRYSGAK
                     KNTNILVVFDGKKDVGSPVEKETFGDIRVYYSHDLTADHCIKSVIRNDRVPANIYVVS
                     SDKEILRYAKNHGCKFFTSEEFSGMIGKELHSVPEEPERDRPLAKEEVAEWMEIFRRK
                     RNAE"
     gene            2429..3448
                     /locus_tag="PK683_02195"
     CDS             2429..3448
                     /locus_tag="PK683_02195"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNH07286.1"
                     /translation="MQNKKKPDRSKEFESLLNLFKKKDNFILTTHRGPDPDGIGAELG
                     LDLLLEKLGKRHFILNADRIADKLSFLDPKSRIRNLEEAKLQNIQTDYTVVIVDNSEI
                     ERIGDVCNYLKPDRSNLIIIDHHDNVDHIPGMYSFPEFSASSEIVYELMLHAGIEPDY
                     NTAVALYLGIVVDTGQFKYNKTSPRTYELAGKLVDYGFPTEETIRKIYEDVSADILYL
                     KRDIFATLEVYKDYGLACIDVRKETYQKYGFNSNPIEGMVSDLLNPRDVYVSLCLSDG
                     DDGLVKMSFRSKGSYNVCNVAKLFGGGGHNNASGAAVKGDFKTVKSNVILKLKEMIRN
                     TKGQV"
     gene            3448..>4166
                     /locus_tag="PK683_02200"
     CDS             3448..>4166
                     /locus_tag="PK683_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07287.1"
                     /translation="MQFNSIAFLFFFTVLYLFYWNLPSFFRRPLLILAGAFFYFSFSK
                     LYLIHFLVVILINFFLLKRIEKDSTKLSVTLAVVFNICNLAFFKYFYFLGKVLFQSTG
                     SDMFADLEKKVTVYFPLAVSFYTFQILSHHIDIYRSREREETSLVNYFLYTLYFPVMI
                     AGPILRGKEFFRNIENASPEKEKIYRGTYLLVGGLIKKVLLADSIGPSVSPVYGNPSE
                     YVWWSLLLVGFIFLMQLYFDFS"
BASE COUNT         1234 a          806 c          973 g         1153 t
ORIGIN      
        1 actccagaag gcgattccag taagagttca tgtaagtgca tatgacaggc cgaaaattta
       61 tatggagatt gtaggtgcca tttccaagac tgataccaat atccttgagg caggagcaac
      121 tgcgacagga gagggaacgg ttcttgcaaa atttcttctg gaaattgaac atctcgatca
      181 gctcaaggaa attctggata atataaaatc cataccgaat ataatcacag tagagaggac
      241 taagacatga agggaatcat tctggcagga ggttcaggaa caagactgta ccctgtcacc
      301 agagtcgtca gcaagcagct gatgcctgta tatgacaagc cgatgattta ttatccgctt
      361 tctgttctga tgctgaccgg gatcagggaa atactgatta tttccacacc gggagattct
      421 cctctttttg aaaaactttt cggtgacggt tcttctctcg gaatccgtat cgagtatgca
      481 gtgcagcctg caccggaagg actcgctcag gcattcctga tcggaaagaa gtttctgaac
      541 ggagaaccct cctgtctgat tctcggagat aatatttttt acggtcatgg aatgattgag
      601 tctctttccg attcagccct tcttcagaat ggaggaatta tttacggtta ttatgtgaat
      661 gatcctgaaa ggtacggtgt ggtcgaattt gactcaaaag ggaatgtact tagcctggag
      721 gaaaaaccgg aaaggccgaa gtcgaattat gctgtccccg gaatttattt ttatgacggg
      781 aaagcttcgg agtatgcaga acagctgaaa ccttccaaaa gaggggaact cgaaatcaca
      841 gacctcaaca ggatgtatct ggaaaaaaaa tctctgaaag tgaatcttct gggcagaggc
      901 gtagcctggc tggatactgg aactcatgag tctctgagtc aggcaggtca gttcatagaa
      961 atcattgaaa agagacaggg tctgaaagta ggatgcattg aagaaattgc atacagaaag
     1021 aaatacataa cgaaagaaca gcttcttgtt catgcagaat caatgattaa aaatgaatac
     1081 ggacagtatt tgaaaaggat agcggatgaa atttaatgat accgagtttg ctgatctgaa
     1141 agtgatcgag ccgacagtgt tcaaggacag cagaggctgg ttttttgaaa gttactcaga
     1201 gaaaaaattt gcagaaaacg gaatcaaatc tgagttcatc caggacaatc atgcactgaa
     1261 ttcagatcct aatacagtca gaggactgca ttttcagaag cctccttttg aacaggcaaa
     1321 acttgtcagg gtcgtcaaag gagcaatttt tgatgtggca gtggatctgc gcagaaactc
     1381 tcccactttc agaaaatggt tcggaattga acttacatcg ttcaacagga cgatgctttt
     1441 tgtacctcgg ggatttgctc acgggtatct ggtgctgaag ccggatacag aggttctgta
     1501 caaggtagac aatctgtaca gtcctgaatc ggaaggcggg atcatctgga atgatcctga
     1561 actgtccatc gactggatgg tgaacagtcc gattctttct cctaaggatg ctgttcttcc
     1621 gccgctctca gagtgtctga atattttcct gtgaatcact atgacaatta tggaaattca
     1681 taatgttgtc tttcacaggg atcggcactg tgtaaaagca aaggaaaaga agactcgcgc
     1741 agagtcgcgg ggttcgcaga ggcttgattt tatcagcgga ttccaaactt gcgattcttt
     1801 tttctctgca tcttcgcgtc cccgtgtgaa aacgatcctt ttacacaacc ccgtctccgg
     1861 caggatcaaa atcagcccgt gtaagaaggt ttcatacagt ctggaaaaac ctttctgcgc
     1921 tccagcctgt ataaggtgga gtaaatgaga attctgatcg acggattcaa tctgatttac
     1981 aaatttcccg atctggaggg actgatgtac gagagcagac tgaatcttgc cagacagggg
     2041 cttctgcaga ttctgcatcg gtattcagga gccaaaaaaa acacgaatat tctggttgta
     2101 tttgacggga aaaaagatgt gggcagtcct gtggaaaagg aaacatttgg agacatccgg
     2161 gtctattaca gtcatgatct gacggcagac cactgtatca agtcagtgat tcgaaatgac
     2221 agagttcctg caaatattta tgttgtcagt tcagacaagg aaattttacg ttatgcaaaa
     2281 aatcacgggt gcaaattttt tacatctgaa gagttttcgg ggatgattgg caaggaactg
     2341 cattcagtgc ccgaggaacc ggaaagagac agacctcttg ctaaagaaga ggtggcggaa
     2401 tggatggaga tattcagaag gaaacgaaat gcagaataaa aaaaagccgg acagatcaaa
     2461 agagtttgaa agccttctga atctttttaa gaaaaaagac aattttatac tgaccaccca
     2521 caggggtcct gatcccgacg gaataggggc tgaactgggg cttgacctgc ttctggaaaa
     2581 actgggtaaa agacatttca ttctgaatgc cgacaggatt gcagacaaac tgagttttct
     2641 tgatcccaag tcaaggatca gaaacctgga agaggcaaaa cttcagaaca tacagacaga
     2701 ctacactgtt gtcatagtag acaattccga aatagaaaga ataggcgatg tctgcaatta
     2761 tctgaagccc gacagatcaa atctgatcat aatagatcat cacgacaatg tggatcatat
     2821 tccgggaatg tactcatttc ctgaattcag tgccagttct gaaatagtat atgaactgat
     2881 gcttcatgcc ggaattgaac cggattacaa tactgcggtt gctctttatc tgggaattgt
     2941 agtggataca ggtcagttca agtacaacaa gaccagcccg aggacctatg aactggccgg
     3001 gaaactggta gactacgggt ttcctacaga ggagacgatc cggaagattt atgaagatgt
     3061 atccgcagat attttatatc tcaaaaggga tatttttgcg acactggagg tatacaagga
     3121 ttacggcctc gcctgcattg atgtcagaaa ggaaacatat cagaaatacg ggttcaattc
     3181 caatccgatt gaaggaatgg tgagcgatct tctgaatccg agagatgtct atgtgtctct
     3241 ctgtctgtct gacggagatg acggacttgt gaagatgagc ttccgttcca aaggatctta
     3301 caatgtatgc aatgtggcca aacttttcgg cggaggaggg cacaacaatg caagcggagc
     3361 tgcagtcaaa ggagacttta agacagtaaa atcaaacgta atcctgaagc tgaaagaaat
     3421 gatcagaaat accaagggac aggtctgatg cagttcaatt ccattgcctt tcttttcttt
     3481 ttcacagttc tgtatctgtt ttactggaat ctgccttcct ttttcagacg tcctcttctt
     3541 attctggcag gtgcattttt ttatttcagc ttttccaagc tttatctgat tcattttctg
     3601 gttgtcattc tgatcaactt tttccttctg aagaggattg aaaaagacag cacaaaactc
     3661 tcagtaaccc ttgcagtggt tttcaatata tgcaaccttg cattttttaa gtatttttat
     3721 tttctgggaa aagttctctt tcagagcaca ggttcggata tgtttgccga tctggaaaaa
     3781 aaggtcacag tctattttcc tcttgccgtc agcttttata cttttcagat attaagtcat
     3841 catatagata tctacaggag cagggaaaga gaagaaactt ctcttgtgaa ttattttttg
     3901 tatactttgt attttcctgt aatgatagcc ggaccgattc ttcggggaaa ggaatttttc
     3961 agaaacatag aaaatgcctc tcctgaaaag gagaaaatct atcgcggcac gtatcttctt
     4021 gtcgggggac tgatcaagaa agtgcttctg gctgattcca taggcccttc tgtttctccg
     4081 gtctacggga atccatccga atatgtctgg tggtcgcttc tcctggtcgg atttattttt
     4141 cttatgcagc tttattttga cttttc
//
LOCUS       DAOQGJ010000087         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11631_length_4155_cov_2.876098, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000087 DAOQGJ010000000
VERSION     DAOQGJ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11631_length_4155_cov_2.876098"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1525)
                     /locus_tag="PK683_02205"
     CDS             complement(194..1525)
                     /locus_tag="PK683_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07288.1"
                     /translation="MIHFLFWILVSSVTLKAWPWNENLHSDWGISAAACHSSQCIEKI
                     QTQLSELKVPRTAVFNRGGVRTVAVFSGRDRVFSRFDETSKKLFETLTKGPSPSAFWI
                     DFGFECPGSFGISLSEAVCSESEKIYTDLDRAHKNKTKVKKLYLDRSVSFDDMQKLLD
                     LKELTEIQISQRLPVYRPFPLDEFPKLERLYLGDAGSLPSAVRIHRKLKSAVLKGSCG
                     QYKGGTSDAVCIREIRSSFLKHVPNLEELDLTDNIIEKIPKDIGRAQKLKKLSLSEIR
                     ETELKKISGLGIEDLTMKNSSLKNISKDVSFLKKLKKLSVSVSAEGGASLSEFPIVSN
                     GKIQELSLIGHYEIMKIPQNIHRMKHLRRLTVIDSDISEIPADISSLKFLEELEIGTV
                     SGRRKKDRTLVLPVSLCSMKKLKRLRITGMRTDRETLRKLEDCLPKVRFDY"
     gene            complement(1549..2136)
                     /locus_tag="PK683_02210"
     CDS             complement(1549..2136)
                     /locus_tag="PK683_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNH07289.1"
                     /translation="MDLIEVKISDITLTNVGFAVFLKPKDVNTSKVVPIFIGPLETHA
                     ITSVLEGIVSPRPMTHDLVNSILNVLNTSVVNITIDQIIDNTFYAKITLRKDEDIFVM
                     DARPSDSIAIALRANAPVFITESILNEAGVEINPSEITGEAVIETSETEIAPKPVTRT
                     ALEILEDSLKAAISSEDYESAAKIRDQIKKMVENS"
     gene            2165..3055
                     /locus_tag="PK683_02215"
     CDS             2165..3055
                     /locus_tag="PK683_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07290.1"
                     /translation="MKAKIMNVEAFFIILNLSFYCSSVFLLFSKESLSFLRTAYIFAG
                     HILLNSILIVGFIDISSSPENYFSMKSRIFDSFFQYSVLSSIFLLLIFLKYRKPIILF
                     SAAELISFFSFFFLFSQEEFSFFSLKFSSVSILKAQNPMILLFAMTCFLPKKIIQRFS
                     FAGLIVFLLIFHQRSLSFFSDRTDSCFYTSYLAPEFFLEEADMLCEGKEGVYTYLKSE
                     KTETAVPVYRFTTKPLRKLDLKTRLPLQKMIVRLKFPIVQLNENSAVIYELSRRYRNE
                     NQIFIMDIQSRKMRMEGPKF"
     gene            complement(3058..3131)
                     /locus_tag="PK683_02220"
     tRNA            complement(3058..3131)
                     /locus_tag="PK683_02220"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3095..3097),aa:Ile,seq:cat)
     gene            3309..>4155
                     /locus_tag="PK683_02225"
     CDS             3309..>4155
                     /locus_tag="PK683_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07291.1"
                     /translation="MKVLLYSVLLLLSACRTSNELTVSKNTADSLSLKGYTQFSIYTG
                     RDVLGYIDTPGQEKTFVKKVWKGKISYAPVRQRIVISNSSENAGDLKMKLQGVFSSET
                     SLDKIREINLVLDNPVEYFLEDIIPEKNALTDAESLKQRFVGAVLKAEKITLEFVTKD
                     GITLRSEADIKSQNISLGTGIKSSTKENSLYVAENVYVGYKMFDPPVNISKLIADANL
                     SSKQSRIAVLPFSAESTDVKDQKYTIGLQEATARALNQIEGIAVLDRNSYQQSLEEEN
                     LGKTNF"
BASE COUNT         1193 a          872 c          826 g         1264 t
ORIGIN      
        1 tttcagggtt tttcgtatcg aggtgggcgg tgaaaatcac tcggtaacct gctttcacac
       61 agtcccaaac tgttcaaagc ctgtcgggag gttcctgggc ttgcagcccc cgcaagctta
      121 taaagctgct aatcacccgc cttttaattc tgttcctgca atcttacaat tctgaaaagc
      181 aggaaaatga agcctaataa tcaaacctga ccttaggaag gcagtcttca agttttctaa
      241 gtgtttctct gtcagttctc atcccggtaa tgcgcagtct tttcagtttc ttcattgagc
      301 agagggagac tggaagaaca agagttctgt ctttcttccg tcttcctgac actgtgccga
      361 tttccagttc ttccagaaat tttagactgc tgatatcggc aggaatttca gaaatgtccg
      421 agtcaatgac tgtaagacgt cttaagtgtt tcatcctgtg gatattctga ggaattttca
      481 ttatttcata atgaccaatc agactgagct cctgaatctt cccgttggat acgatcggaa
      541 actcggaaag agatgcacct ccctctgcgg aaacacttac gctgagcttt ttcagcttct
      601 tcagaaagga gacgtctttt gagatatttt ttaatgatga atttttcata gtcaggtctt
      661 caatgccgag tcctgaaatt tttttcagct cagtttccct aatttccgac agagaaagct
      721 ttttaagttt ctgcgccctt ccgatgtcct ttggaatttt ttcaataata ttgtctgtca
      781 gatcaagctc ttcaagattc ggtacatgct tcagaaagga tgatctgatt tcacggatgc
      841 aaaccgcatc tgaggttcct cctttgtact gcccgcagga acctttcaac acagcagatt
      901 tcagctttcg gtgaattctg actgcagaag gaagacttcc tgcatctccc agatacagcc
      961 gttcgagttt gggaaattca tcgagaggaa aaggtctgta tacaggaagc ctttgactga
     1021 tctgaatctc agtgagttcc ttcagatcca gaagtttctg catatcgtca aagcttaccg
     1081 atctgtccaa gtaaagcttt ttcaccttag ttttgttttt atgtgctctg tccagatcag
     1141 tgtatatttt ttccgattca gaacagaccg cctcggacaa tgaaatcccg aaagaaccgg
     1201 gacactcaaa cccgaaatct atccagaagg cagaaggaga aggtcctttt gtcagagttt
     1261 caaaaagctt tttcgatgtt tcgtcaaatc ttgaaaatac cctatccctt cccgaaaata
     1321 ccgcaaccgt cctgactccg cctctgttga agacggccgt tctgggaacc ttcagttcgc
     1381 tcagctgggt ctgaattttt tcgatgcact gtgaagaatg acaggctgcc gcagaaatac
     1441 cccagtcgct gtgaagattt tcattccaag gccaggcctt gagggtaaca gatgaaacca
     1501 aaatccaaaa aagaaaatga atcactttat tctctgttca ggattgcttc agctgttttc
     1561 gaccattttt ttgatctgat ccctgatttt tgctgcagac tcatagtcct ctgaagagat
     1621 tgctgccttc agagaatctt cgagaatttc caaagcggtt cttgtaacag gtttgggagc
     1681 aatttccgtt tcagaagttt caatgacagc ctcaccggta atctcggaag gattgatttc
     1741 cacaccggct tcattcagaa tgctttcagt aataaacaca ggggcatttg cacgaagtgc
     1801 aatcgctatc gaatcggacg gacgggcatc catcacaaaa atatcttcat cctttcggag
     1861 agtaattttt gcataaaaag tattgtcaat gatctgatcg attgtaatgt tcaccacaga
     1921 tgtattcaga acattcagaa tgctgttcac aaggtcatga gtcataggtc tgggagaaac
     1981 aattccttcg agaacactcg taatcgcatg agtttcaaga ggaccgatga aaattgggac
     2041 taccttggag gtattgacat ctttgggttt gagaaaaact gcaaatccga cattcgtcag
     2101 agtaatatca gaaattttta cttcaatcaa atccatattt acaagttaga agacttcatt
     2161 ttttttgaaa gctaaaatca tgaacgtgga agcttttttt ataatactga atctgagctt
     2221 ttactgcagt tctgtttttc tgcttttttc caaggaatcc ttatctttcc tgaggactgc
     2281 ctacattttt gcaggacaca ttctgctgaa ttccatactt atagtcggat ttattgatat
     2341 ttcttcctct cctgaaaact atttttcaat gaaatccaga atttttgatt ccttttttca
     2401 gtattctgtt ctttcctcaa tctttctgct gcttattttt ctgaaataca gaaagcccat
     2461 catccttttt tcggcagctg agctgatttc ttttttcagt ttcttttttc tgttttcaca
     2521 ggaggaattc agttttttca gtctcaaatt ttcatcggtc tccatactga aagctcagaa
     2581 tccaatgatt ttgttattcg caatgacctg ttttttgcct aaaaaaatca tccagcgttt
     2641 cagttttgca ggactgattg tttttcttct catttttcac cagagaagcc tttctttttt
     2701 ttccgacaga accgactcct gtttttatac atcctacctt gctccggaat tctttctgga
     2761 ggaagccgat atgctctgtg aagggaaaga gggagtttac acatatctga aatcggaaaa
     2821 aacagagacc gctgttcctg tttaccgatt caccactaaa ccgttaagaa agctggatct
     2881 gaagaccaga ctgcctcttc agaaaatgat tgtaagactg aaattcccta tagtgcagtt
     2941 aaatgaaaac agtgcagtaa tctatgaact gtcgagaaga tacagaaacg agaatcagat
     3001 tttcataatg gatattcaaa gcagaaaaat gagaatggaa ggaccgaaat tttaagatgg
     3061 gcccagaagg actcgaacct tcgacccgca gattatgagt ctgccgctct aaccgactga
     3121 gctataggcc ctcgatgtca ttttttttta gagtttcaaa aatacaatct aaaaaaaatt
     3181 gattttaaaa acaggaatct tcagaactga acgttcaatc tgcaaatcat ttttcaaggg
     3241 cttcatctaa aaatgatatt gaagtttatg actgaatgca taatttgtct taagaaaaga
     3301 gagtcatcat gaaagtactt ctatattcag ttcttttgct tctgtcagcc tgcagaacat
     3361 caaatgaact tactgtaagt aaaaatactg cagacagtct gagtctgaaa ggatatactc
     3421 agttcagtat ttatacaggc cgggatgtac ttggatacat tgatactccc ggtcaggaaa
     3481 agacttttgt gaaaaaagtc tggaaaggca aaatcagtta tgctcctgtc aggcagagaa
     3541 tcgtcatttc caactcttcc gaaaatgcag gagacctgaa aatgaagctt cagggagttt
     3601 tttcatcaga aacttccctg gacaagatac gtgaaatcaa tcttgttctg gacaatcctg
     3661 tggaatattt tctcgaagac attattcctg aaaaaaatgc tctgacggat gcagagtccc
     3721 tgaagcagag atttgtaggc gcagtgctca aggcggaaaa gatcactctg gaatttgtca
     3781 caaaagacgg gatcaccctc agaagtgaag ctgatatcaa gtctcagaat atcagtcttg
     3841 gaacaggcat aaagtccagc acaaaggaaa actcgcttta cgtcgcagaa aatgtgtatg
     3901 tgggatataa aatgtttgat ccgcctgtaa atatatccaa acttattgct gatgcgaacc
     3961 tttcgtcaaa acagtccagg atcgctgttc tacccttttc tgcagaaagc actgatgtca
     4021 aggatcagaa gtatacaatc gggcttcagg aagcaactgc acgggcactg aaccagattg
     4081 aaggcatagc agtcctcgac aggaacagct atcagcagag cctggaagag gaaaatctcg
     4141 ggaaaacgaa tttcg
//
LOCUS       DAOQGJ010000088         4130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11752_length_4130_cov_3.533006, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000088 DAOQGJ010000000
VERSION     DAOQGJ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11752_length_4130_cov_3.533006"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(516..908)
                     /locus_tag="PK683_02230"
     CDS             complement(516..908)
                     /locus_tag="PK683_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07292.1"
                     /translation="MNTYKLPESALLILILLFNNCTGSKEIKVGGPCEYEDFPGQILI
                     NSVEPDKTDSKKAIVKFDFSPIDNSRKYRFGNFKDTGIHLLDGNENPDKNKIEFWGVK
                     KGKNFPAIRKEIKFGACTPVIFEVPSFK"
     gene            1172..2623
                     /locus_tag="PK683_02235"
     CDS             1172..2623
                     /locus_tag="PK683_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter, Ompp1/FadL/TodX family protein"
                     /protein_id="HNH07293.1"
                     /translation="MKRQVRKIAVLLGLLHSFPAFSDAYHNINGFFGERAAGLGGAYT
                     AVSDDPSGAFYNPAGLNLAYDNSISLSASNYTKVKKTYENALGPAQGYGRTNQNYIPN
                     FFGMVKEVGAGKFAFSVVNPIQENYQRNDQIRLPLFYPDISNIKSYNRETYNQLLVGA
                     SYSRPITSKLSWGATLYYVNDTASITSTQLTMFKNKQYAMQTINDNRRTTGLLPVLGL
                     MFNLTDKISLGASLRKTFVMGQNRLINAITMDSSSTSTDGITFLEGTQKAFGASVGNT
                     IYKGPAYTGRVPETYELRLGGAFFPTKKLMIATDLIYTSGYSFKKNNTEVITSPSANI
                     IILTDKDDLELKRYQTWNIATGVEYFLTDFLALRLGVYTNNANTKKIDWMTSALQAYN
                     RAAGDTETIASSGGNSVQFRIPTLRDNPRNEYVNTMGYSFGFSFATAKAALSLSIIKE
                     HGKGAAQLDSMRPAQQLTYDASSIYLVITSRSN"
     gene            2625..>4130
                     /locus_tag="PK683_02240"
     CDS             2625..>4130
                     /locus_tag="PK683_02240"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNH07294.1"
                     /translation="MKNKAKLTFGGGLFLLSLIVVGILDLFGGLIKIEDSLIDIRHRM
                     VNPNHKFTDKVIVLDIDEQSLETYKAEPLFGRWPWRRAIYGPILKYIAAGGAKLILFD
                     IMFFEQSSSCNLDDDTQLALATAEAGIVSHAVNFRTDEMTEEEKKIYAPPAYVLSQAY
                     PVEKPENLKLKGFNRINYPVESILQAMPKLHSVFYAQDEGVARYYLPFHKYEDKYFPT
                     LSTQIYRAFHPVEKADADWNSLNLYSQGKELNIPLVNGKYRIHYYPEKTIDDPSLRIS
                     ISGVIDSIRSLETKEVTDPEKTKVPLSRFKDKVVLIGVSAASGFDIRTTPYGDKPGFL
                     YHAITASNFLEKHFMKIPPGFLGYLIVAVSVGICLALSLFSAGTITRIAVPLAVFIAY
                     PVIAFLVFKMDFMAPLAGFAVAYPLAFLGSQAYLSFTEGAEKRKYSKVLSNMVDPTIV
                     GEALEDLEALKKGGEKEITAFFSDVAGFSTISEQLTSADLASLLNEYLSAMT"
BASE COUNT         1198 a          815 c          896 g         1221 t
ORIGIN      
        1 gttttcaaaa gtctttgcgt atttatttgt tcacgactca gtaaaatcaa gtctgccatc
       61 cgggaaaagc gcaattattt atgaaaatga gtataatcag aggtctccct ctcttaaaat
      121 ctaaaggaat gttggagatc ctacaaggct gcgagggcat taggatttta gcttgattaa
      181 gatttcagag aaggttacta taaactcacg ttagacaaat acgatatact gtctagatgc
      241 tgtaattttc agaaaacatt agcaaaatct agcagtttaa cagcttgcga tgaatcagta
      301 aaaacagcag cttccacgct tgcaggattt taggcagttt cagagccgcc gaaccggttt
      361 cagatattgg cgagggcaga gctctgagca gtttctaagt ttgcggcagc ttcaaagtct
      421 gcgaaaccgc cggtagactt atgccaaaaa cgccccacct gcaaccgtag cgtaaggtga
      481 gttattcaga tgaccacaga tcaatagacc aaattttact taaaacttgg tacctcaaat
      541 atcacaggtg tgcatgctcc gaatttgatc tcttttctga ttgctggaaa attctttcct
      601 tttttgacac cccagaattc aattttattt ttgtccgggt tttcgtttcc atccagcaga
      661 tgaattcctg tatctttgaa gtttccaaat ctgtatttcc tggaattgtc gataggagag
      721 aagtcaaact tgactatagc tttttttgaa tcagttttat ccggttctac cgaattaatg
      781 aggatctgtc ctggaaaatc ttcgtattcg cagggacctc ctactttaat ttcttttgaa
      841 ccggtacaat tattgaaaag cagtattaaa atcaataggg cagattcggg aagtttatat
      901 gtgttcatgg aatctattga attatttctg attggaatcg tcaagaagaa cggagatcat
      961 tttctctttc gaattttaaa cttgacagac tttttttcag tcgataaagc attagtttat
     1021 gaagtagtct gcttgactga atagtgcgga taaataagat aactgactga aacgggaaaa
     1081 aagaaacatc atctctagga aaggtttgaa tgagtctgtt ttcgaaaatt ttttgtttca
     1141 aaaggaattg ccacataaag gagatgaatc aatgaaaaga caagtgagaa aaatagcagt
     1201 gctgttagga ctgctgcatt cttttcctgc tttttctgat gcctatcaca acatcaatgg
     1261 atttttcggt gagagagctg ccggtcttgg cggtgcctat actgcggtat cagatgatcc
     1321 ttccggagcg ttttacaacc cggccgggct gaatttggct tatgacaact cgatttctct
     1381 ttctgccagc aattatacaa aggtcaagaa aacatatgaa aatgcacttg gtccggctca
     1441 aggttacggc agaacgaatc agaactacat accgaatttt ttcggtatgg tgaaggaagt
     1501 tggggcaggg aaatttgctt tttccgttgt aaacccgatt caggaaaact atcagagaaa
     1561 tgatcagatt cgtcttcctc tgttttatcc tgacatttcc aatattaaaa gttacaatag
     1621 agagacatat aatcagcttt tggtaggggc gtcttattcc agaccgatta ccagcaagct
     1681 ttcgtggggt gcaacgcttt actatgtaaa tgacacggcg agcatcactt ccactcagct
     1741 tacaatgttc aagaacaagc agtatgcaat gcagacaatc aatgacaaca ggaggacaac
     1801 aggacttctt ccagtgcttg gacttatgtt taatctgact gataaaattt ccttaggcgc
     1861 atctctcaga aaaacatttg taatgggcca gaaccgtctg atcaatgcga ttacaatgga
     1921 ttcttcatcc acatccactg atgggattac ttttctggaa ggaactcaga aggcattcgg
     1981 tgccagtgta ggcaacacaa tttacaaagg tcctgcgtat acaggaagag ttcctgaaac
     2041 ttatgaactg agactcggcg gtgccttttt ccctacaaaa aagctgatga tagcgacaga
     2101 tcttatttat acttccggtt actcattcaa gaaaaacaat acggaagtca tcacttcacc
     2161 ttcagcaaac atcattattc ttacagacaa ggatgatctt gaactgaaaa gatatcagac
     2221 atggaacatt gcgactgggg tcgaatactt tcttaccgat tttcttgccc ttcgtctcgg
     2281 tgtgtataca aacaatgcaa atacaaagaa aattgactgg atgacatcag cgctgcaggc
     2341 gtacaaccgt gcagcgggtg acactgaaac gattgcttct tcaggaggga attctgttca
     2401 gttcaggatt ccgacgctca gagacaatcc tagaaatgaa tatgtaaata caatgggata
     2461 cagtttcggc ttctcgtttg ccactgctaa ggcggctctc tctctgagca ttatcaaaga
     2521 gcatggaaaa ggcgcggctc agcttgattc aatgagacct gctcagcagc tgacctatga
     2581 tgcatcatcc atttatctgg tcataacatc caggagcaac tgatttgaaa aacaaggcga
     2641 agcttacatt tggcggcgga ctttttcttt taagtctgat cgttgtaggg atacttgacc
     2701 tctttggcgg gctgatcaag attgaagaca gtctgatcga catccgtcac agaatggtca
     2761 atccgaatca taagtttaca gataaagtca ttgtactcga cattgacgag cagagtctgg
     2821 aaacatacaa ggcggaaccc ttgtttggac gctggccttg gagaagggca atttacggac
     2881 cgattctgaa atatatcgct gcaggcgggg caaagctgat tctgttcgat ataatgtttt
     2941 ttgaacagtc atcatcctgt aatttggatg atgatactca gcttgctttg gcgactgcag
     3001 aggcagggat tgtatctcat gcggtcaatt ttagaacgga tgaaatgact gaggaggaga
     3061 aaaagattta tgctcctcct gcatacgttc tgagtcaggc atatccggta gaaaaacctg
     3121 aaaatctgaa attgaaggga ttcaacagaa tcaactatcc tgtagaatcc attcttcagg
     3181 caatgcctaa acttcattca gtgttctatg ctcaggacga aggggtggcg agatattatc
     3241 ttccatttca taaatatgag gacaagtatt tcccgactct gagtacacag atttacagag
     3301 catttcatcc tgtggaaaag gctgatgcag actggaattc tctgaatctc tattcgcagg
     3361 gaaaagaact gaatattcct cttgtgaacg gaaaatatag gattcattat tatcctgaga
     3421 aaacaataga tgatccttct ttgcggattt cgatttccgg tgtcattgat tctatcagaa
     3481 gtctggaaac taaggaagtg acggatcctg aaaaaacaaa ggtacctctt tcccgattca
     3541 aggacaaggt tgttttaatt ggagtttccg ctgcatccgg ttttgatatt cggacgactc
     3601 cctacggtga caagcccggg ttcctgtatc atgcaattac agcatccaat ttcctggaga
     3661 agcattttat gaaaatccct cctggatttc taggttatct gatagttgca gtttctgtgg
     3721 gaatctgtct tgcactttct cttttcagtg caggaactat tacaaggatt gcagttccgc
     3781 tggctgtgtt cattgcatat cctgtgatcg cattccttgt tttcaaaatg gattttatgg
     3841 ccccgctggc aggatttgct gtggcttatc ctttggcatt tctaggatct caggcatact
     3901 tgagttttac ggaaggtgca gaaaaaagaa agtacagcaa ggttctttcg aatatggtgg
     3961 atcctacaat tgtgggagaa gcacttgaag acttggaagc actgaaaaaa ggcggtgaga
     4021 aagaaattac agcctttttc tccgatgtgg ccggattctc cacaatttcc gaacagctta
     4081 catctgctga tctggcttct cttttgaatg agtatctttc tgcaatgacc
//
LOCUS       DAOQGJ010000089         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11786_length_4125_cov_2.942506, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000089 DAOQGJ010000000
VERSION     DAOQGJ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11786_length_4125_cov_2.942506"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1137)
                     /locus_tag="PK683_02245"
     CDS             complement(139..1137)
                     /locus_tag="PK683_02245"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH07295.1"
                     /translation="MNKKKILLSVPVIAVIGFFSGPKVDQSVTVKEIAVSENPEEFIQ
                     NRESAFKDIKPDSEKKIIWYDSSLKKKTEYSIVYIHGYSANRLEISPVCDILAKNLKA
                     NLFYTRLSGHGREDMNAFADATVNDWINDAVEAVRVGEKLGEKVIIIGTSTGATLGTW
                     IASEKEYRKNIAAFVFLSPNFHPHSRAIRIGLYPWGRQIARLILGENREWKSDNEKVM
                     KHWNTKYPSRAVVNLIGLVDYVSRKDVSGSEVPILVMYSPKDTVISTEEVQKRFQDFG
                     GKHRQIVPFTDSQDVNQHVLAGDIISPSTNGKAVEIIEKFFSDAGIVQPEPVLKKP"
     gene            complement(1198..1653)
                     /locus_tag="PK683_02250"
     CDS             complement(1198..1653)
                     /locus_tag="PK683_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNH07296.1"
                     /translation="MKLFEALLKKEGLKVTAQRLLVAETIFSIHSHFTADSLLDMFRD
                     RRDEISKATIYRILGIMVEAKLLMEHNFGKDYKFYEHIVGHSHHDHIICSDCGRIEEF
                     MDERIEAIQEEVCSKKGFKIKGHSLNVYGECLKKQDCEHFKASEKAPKK"
     gene            complement(1710..2681)
                     /locus_tag="PK683_02255"
     CDS             complement(1710..2681)
                     /locus_tag="PK683_02255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HNH07297.1"
                     /translation="MKAFVAENWCEPEELVYREENDPTVEDHQVLIEVYAAGVNFPDL
                     LLIQGKYQLRPKRPFIPGAEVSGVVIRTGKSVTKYKEGDKVYAVSFLGGYAEKVSVPE
                     KDVYPLPEGMSFEEGAGMVITYQSSYFGVVVRGKLLPNETLLVHAGGGGIGTSAIQIG
                     KAVGAKVIATAGSEEKLEIAKKCGADIVLNYNDSQWSKKIRKEYGGADVVIDPVGGDI
                     FEKSVLCLNFEGRIIVVGFTSGKIPEIAVNKLLLANASIVGLYWNLYQRDFPEKIEKC
                     IQDLHKWYLEGKIKPVIYKTYSLQEAPIALRDVTGRKSYGKAILKIR"
     gene            3297..4118
                     /locus_tag="PK683_02260"
     CDS             3297..4118
                     /locus_tag="PK683_02260"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNH07298.1"
                     /translation="MKATSKTIWGVPAYLPYLQPKLTEKRILSAEKKLGFRLPKAFLD
                     LLKVQNGGYIRFSLPDSPHRMICGIGPNFPSLLRTNLDEVQEYVSFPLKGLIPFDGDG
                     HWHLCLDYRKNPKSPSVTYIDIECDNQKTIAKSFADYLKLLVIDTEDDYVLESVSDIA
                     ELKKAVSKALKKEFDPPDLWSYGYLVHRIALGSKRKPEWLWISPNEVPAGFVREEDAR
                     YKTLKKQMKGTALKYPFLSSNSFTLSTTPAVRSRVLEIFSKLGYPPKPMKDFLKT"
BASE COUNT         1190 a          938 c          754 g         1243 t
ORIGIN      
        1 gatgcagtga ctgtccagcc atttctgtca accatcctcc attcaccttc aatttcatct
       61 gcaggcaggg acagagcata tgaaacagaa aacaaaatat agaatatata ttttttcatc
      121 atacttcaga accctgaatc agggtttttt cagaacaggc tcaggctgga caattcctgc
      181 atcactgaaa aatttctcaa tgatctccac tgccttgccg tttgtggaag gagaaataat
      241 atccccggcc agtacatgct gattcacgtc ctgtgagtca gtgaagggaa caatctgtct
      301 gtgctttcct ccaaagtcct gaaaacgctt ctgcacttcc tctgtactga tcacggtatc
      361 tttcggagaa tacattacaa gaatcggaac ctctgatccg gaaacatcct ttctgcttac
      421 atagtccaca agtccaatca gatttacaac tgcccttgaa ggatattttg tattccagtg
      481 tttcattact ttttcattat cagatttcca ttctctgttt tctccgagaa tcagacgggc
      541 aatctgcctt ccccagggat aaagcccgat tcgaatcgcc ctggaatgag gatgaaaatt
      601 cggtgagaga aatacaaatg cagcaatatt ctttctgtat tctttttcgg acgctatcca
      661 ggttccgaga gtagccccgg tggatgtacc tatgataatc accttctctc caagtttttc
      721 gccgactctt acagcctcga ctgcatcatt gatccagtca ttgacggttg catctgcaaa
      781 tgcattcata tcctcccgtc catggccgga aagtctggta taaaaaagat ttgctttaag
      841 atttttggca agaatgtcgc agacgggaga gatttccagc ctgtttgcac tgtagccatg
      901 aatgtataca atggaatact ctgtcttctt ttttaatgaa gaatcatacc atataatttt
      961 tttttctgaa tcaggcttaa tatccttaaa agcagattct ctgttttgga taaattcttc
     1021 aggattctca gagacagcaa tttctttgac agttacagac tgatcgactt taggtccgct
     1081 gaaaaagccg ataacggcaa tgacaggaac ggataacaga attttctttt tattcataca
     1141 ggcaccgaag attgtttctg ttaaatatct tcggcagttc tgtattgaaa agaaaaatta
     1201 tttcttcgga gccttttcgg acgctttaaa atgttcgcag tcctgctttt tcaggcattc
     1261 tccgtaaaca ttcaggctgt gcccttttat tttgaagccc tttttggagc acacttcctc
     1321 ctgaattgct tcaattctct catccataaa ctcttcaatt ctgccgcagt ccgaacaaat
     1381 aatatgatca tgatgtgaat gccctacaat atgttcataa aacttgtaat cctttccgaa
     1441 attatgctcc ataagaagtt tcgcttccac cataattccc agaattctgt aaattgtagc
     1501 tttggaaatt tcatctcttc tgtcacggaa catatccaga aggctatctg ctgtgaaatg
     1561 gctgtgaatc gaaaaaattg tctcagcaac aagcagtctc tgggctgtca ccttcagtcc
     1621 ttcttttttc agaagggcct caaacagttt catttcctct tcaacatcct ggaattcgcc
     1681 ggttcttttc ttaactgtct tcttcttgtt catctgattt ttaaaatcgc cttcccgtaa
     1741 gattttctcc cggtaacatc ccgcaaagca atgggcgctt cctgcaaaga ataggtttta
     1801 taaataacag gttttatttt cccttccaaa taccacttat gaagatcctg aatgcatttt
     1861 tctatttttt caggaaaatc tctctgatac agattccaat ataggcctac aatgctggca
     1921 tttgccagaa gcagtttatt cactgcaatt tccggaattt ttccgctggt aaaaccgacc
     1981 acaatgattc ttccttcaaa attcaggcaa agcactgatt tttcaaaaat atccccgccg
     2041 accgggtcaa tgacaacatc agctccgcca tactctttcc tgattttctt ggaccattga
     2101 gagtcgttgt aattcagaac aatatctgct ccgcatttct tggcaatttc aagtttctct
     2161 tcagacccgg cagtggcgat gacttttgcc ccgactgctt ttccgatctg aatggcggat
     2221 gttcctattc ctcctccgcc tgcatgaacg agaagagttt catttggaag aagttttccg
     2281 cgcacaacaa caccaaaata ggaagactgg taagtgatga ccattccagc accttcctca
     2341 aaactcattc cctcaggcag aggatagaca tctttttcag gtacggatac tttttcagcg
     2401 taacccccga gaaaactgac tgcatatact ttatcgccct ctttgtactt tgtaacggat
     2461 ttccctgttc ttatgacaac acccgatacc tctgcacctg gaataaatgg ccgtttaggg
     2521 cgaagctgat atttcccctg aataagaaga aggtccggga aattgacacc ggcagcatac
     2581 acttcaatga gaacctgatg atcttcgaca gtcggatcat tttcttccct ataaacaagt
     2641 tcttccggtt cgcaccagtt ctctgctaca aatgccttca tgaatcctgt attattatta
     2701 cggtaatctg agttcaagca taaaaatcaa agatccataa aattaaacct gataaatatc
     2761 acattccgct taaaataccg gcatatgaat gcaaaaactg aaatatacgg cagttttcag
     2821 catattcatt ttcttatgcc tgcactttaa gcatcagaat atgagaaata cggaattgac
     2881 atcaattgat gaacagcata tcgctccgtc aatgatgagt ttaaccggaa gtagaataat
     2941 tattgataaa aatttcgata ttaggaggga ataaaattaa aaatacagat cattccgaat
     3001 tagaattcag tatattccct tttatttcac tcaccttacg caggcagcag gaagtatttg
     3061 gcaggacttt gaacctgctg gattttcctg aatctttctg aaaattgcag agtctggctg
     3121 gcagatcgta tttgcgcaag gtgagttata tcagaaaatc ctgcttcatc acctatgctc
     3181 tttttccatt tcttttcaaa agtcctgatt ccctgctttc agcttcaagg ctcaagcccc
     3241 ttactgaaaa acaattgact caattcagta aatcagggaa gttagaacag aaaataatga
     3301 aagctacctc aaaaacgatc tggggcgttc ccgcctatct cccgtatctt cagccgaaac
     3361 tgactgaaaa aagaattctg tcagccgaaa agaaacttgg gttcagactg ccgaaagcat
     3421 ttctggatct tctgaaagta cagaacggag gatatatccg tttttctctg ccggacagtc
     3481 ctcaccgtat gatctgcgga atcggtccaa attttccctc tctgcttcgc acaaatctgg
     3541 atgaggttca ggaatatgta agttttcctc tgaaaggact gattccgttt gacggggacg
     3601 gacactggca tctatgcctg gattacagaa aaaatccaaa atcgccgtct gtcacctaca
     3661 ttgatataga atgtgataat caaaaaacta tagcaaaaag ttttgccgac tatctcaagc
     3721 ttcttgttat tgatacggaa gacgactatg tgctggaatc cgtatcggac attgctgaat
     3781 tgaaaaaagc agtatccaaa gctctgaaaa aagaatttga tccgcctgat ttatggtctt
     3841 acggttatct ggtccacaga atagcactgg gttccaaaag aaaaccggaa tggctctgga
     3901 tcagcccgaa cgaagttcct gcaggatttg tacgtgaaga ggacgcaaga tataaaacac
     3961 tgaaaaaaca gatgaaagga actgccctca aatatccgtt tctcagctcc aattctttta
     4021 cactgagcac gacaccggca gtaagaagca gagttctgga aattttctca aaactgggat
     4081 acccgcctaa gccgatgaag gattttttaa aaacctgaaa ttttt
//
LOCUS       DAOQGJ010000090         4119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11804_length_4119_cov_3.634596, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000090 DAOQGJ010000000
VERSION     DAOQGJ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11804_length_4119_cov_3.634596"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PK683_02265"
     CDS             <1..359
                     /locus_tag="PK683_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNH07299.1"
                     /translation="APGDQHMKVVRSGLGYAVSLDRADKVSGHRPSVDVLFRSISELP
                     VCRKTVGIILTGMGRDGAQGLLEMRKRGARTVGQDEESCVVYGMPREAFLLGAVEKQI
                     PLHGISDDLCSLLGAV"
     gene            361..1659
                     /locus_tag="PK683_02270"
     CDS             361..1659
                     /locus_tag="PK683_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07300.1"
                     /translation="MNIDILVVDDESTVLFTMHELICSLGYTCDCEADPEAAVEKIKN
                     NKYKIMITDLQMPSMKGEELLEKVRSADEDIVCIVATGVTDIRRIVSSLSEYKAYDFM
                     MKPVDRLFLKDRIEKAMELVSLRKKIGELVTNEAVFFQDILKVFDWKKELQKQKFESF
                     ASKIIRQLNIGLFHGGGIGGLLSVLSLVLGKIQKNPDSGKYELTEKQYQMIKENYAMS
                     KEIAAGFSKAQSCLMDDSVYEDFISVSDLPAVFEQYKSALDPMLKIKEQKLSISSIPS
                     GARTKKVLFDREAMRSVFSELLINAMKYSEFGDTIYVIFFIHDKNVEIKILNPAYPNQ
                     DGTVGIAGNNETMIFEPFFRLSNVAVEGYYMEEFSAGIGLAVVRKILEKHNSSINIFT
                     VKDHQRNRQGSDISVTVSLPFLSAETLDGAYSKGLKEWAV"
     gene            1656..2012
                     /locus_tag="PK683_02275"
     CDS             1656..2012
                     /locus_tag="PK683_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07301.1"
                     /translation="MKRILAVDDAPVVLKIIDFALSDEGFEVVQAGNADQAKKILQKE
                     KFDIGIFDVNMPGQNGIDLTRDVLKMENGKAMKIVILTTESSEDLQNQGKNAGAVGWI
                     VKPFEGGDLLELVKML"
     gene            2076..2966
                     /locus_tag="PK683_02280"
     CDS             2076..2966
                     /locus_tag="PK683_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNH07302.1"
                     /translation="MGGFEHSFDSDNFSLESVLQEFFDDSAYSKNTDSLIKVAAGKIP
                     WDFPVYDLIEDIKDSTLRLKMRSSSRDLQLAMIEYHLHFLTKKEKIGSLYKELEKFVF
                     LLSLIGDENASYLDFKSELDRMAFRVGELIALNRASVSDSAKLQILTQVIHEEERFIG
                     NQIFYQNPDNSFLTKVLKTKLGIPITLSIIYILIGLRLELPLFGINMPLHFLVSYEND
                     DFQTYVDPFNGGVWIDKDTCLRFLEANGYSTETDMFPVASTSSIIRRMYNNLILIYRN
                     QNEKTLENILIRQASILQPA"
     gene            2993..3682
                     /locus_tag="PK683_02285"
     CDS             2993..3682
                     /locus_tag="PK683_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07303.1"
                     /translation="MLKNSQMLFFIKTSFLIIFLFCFGVFSQTNDPSDPKNSIKNLTQ
                     THTVVNTVDLTANSKKILAIGADSKQIEYKLTGETVSTLKEFGNMGLVITDKSILAVS
                     VNASEFARQPFFGDVNINYTVSNSIIFVSTNRKVYIYSVSSSEWKTVDIAGEYVRTQA
                     AFDRTGTLITNKRIIAYSDYIKDVRSYPINNMVPVYSCTVMDNKIVYDSIQKTIIFRA
                     AAGDFTVNSFN"
BASE COUNT         1256 a          745 c          908 g         1210 t
ORIGIN      
        1 ttgctcccgg agatcagcat atgaaagtag tcagatccgg actgggatat gctgtatctc
       61 ttgacagagc ggataaggtt tcagggcaca gaccctcagt ggatgtgctt tttcggtcca
      121 tatcagagct tcctgtctgc agaaagacag tcggaatcat tctgactgga atgggcagag
      181 acggggctca gggccttctg gagatgagga aacgcggtgc cagaacagta ggacaggatg
      241 aggagagctg tgtagtttac ggaatgccaa gggaagcatt cctgctggga gccgtggaaa
      301 agcagattcc gctgcacggc atttctgatg atctctgcag tctgctggga gcagtctgaa
      361 atgaatattg acatacttgt agtggatgat gaatcaacag ttctttttac aatgcatgaa
      421 ctgatctgtt cattgggata tacctgcgac tgtgaagctg accctgaagc tgctgttgaa
      481 aaaataaaga ataataagta taaaataatg attacagatc tgcagatgcc ttcaatgaaa
      541 ggagaggaac ttctggaaaa agtccggtct gcagacgaag acattgtatg cattgttgcc
      601 accggtgtca ccgatattag acggattgtc agttccctca gtgaatacaa ggcatatgac
      661 tttatgatga aacctgtgga cagacttttt ctgaaggaca gaattgaaaa ggcgatggaa
      721 ctcgtgtccc tccggaaaaa aataggggaa ctggtcacta atgaagctgt attttttcag
      781 gacattctga aggtatttga ctggaaaaag gaactgcaga agcagaagtt tgaatcattt
      841 gcatccaaga taatcagaca gttgaatata ggtctttttc acggcggcgg gataggcggt
      901 cttctttcag ttttgagtct tgttcttgga aaaattcaga aaaatccgga ctcaggaaag
      961 tacgaactga ctgaaaaaca gtaccagatg attaaggaaa actatgcaat gtccaaggag
     1021 attgcagcag gtttcagcaa ggcacagtcc tgtctgatgg atgattcggt ctatgaagat
     1081 tttatcagtg tctcagatct gccggcagtt tttgagcagt acaagtcagc gctggaccca
     1141 atgctgaaga taaaggagca gaagctttcc atcagttcca ttccttccgg agcaaggaca
     1201 aagaaggtgc tttttgacag ggaggcaatg agatcagtat tttcagagct gctgatcaat
     1261 gctatgaaat actcggaatt tggggatacc atatatgtta tattttttat ccatgataaa
     1321 aatgtggaaa tcaaaattct gaatcctgct tatcccaatc aggacggaac ggtcggaatt
     1381 gcagggaaca atgaaacgat gatttttgag ccgttcttca gactcagcaa tgttgcagtt
     1441 gaagggtatt atatggaaga attttctgca ggaatcggtc tggcagtagt caggaagatt
     1501 ctggaaaaac acaactcatc cataaatatt tttacagtaa aagaccatca gagaaacagg
     1561 caggggagcg atatttcggt tacagtcagt cttccttttc tgtcagccga aactcttgat
     1621 ggtgcatatt caaaaggact taaggagtgg gcagtatgaa aagaattctt gctgtagatg
     1681 atgcaccggt tgtactgaaa attattgact ttgcactgtc agatgaaggc tttgaagttg
     1741 ttcaggctgg aaatgcagat caggccaaaa agatactgca gaaagagaaa tttgacattg
     1801 gaatttttga tgtcaatatg cccggacaga acgggattga tctgactaga gatgtactga
     1861 aaatggaaaa cggaaaggca atgaaaattg tcattctgac tacagaatcc agtgaagatc
     1921 tgcagaatca gggaaaaaat gcaggtgcag tcgggtggat tgttaaacct ttcgaaggcg
     1981 gagaccttct cgaactagta aaaatgctct aaaaaactga gtacaagact aaaaagcata
     2041 ttgactgttt ttaccctccg gtcgaaaatt aggctatggg cggatttgaa cattcttttg
     2101 acagtgacaa tttttctttg gaatctgttc ttcaggagtt ctttgatgat tccgcatact
     2161 ctaaaaatac cgattctctg attaaggttg ctgccgggaa aattccttgg gatttccctg
     2221 tttacgatct gattgaagat attaaggaca gtacactccg tctgaaaatg agaagttcct
     2281 ccagggatct tcaactggct atgatcgaat accatctgca ttttctgaca aaaaaggaaa
     2341 aaatagggag tttgtacaag gaattggaga aatttgtatt ccttctttct ctgatcggcg
     2401 atgaaaatgc ttcctatctg gatttcaagt ctgaactgga caggatggcg ttccgggtag
     2461 gcgaactgat tgcactgaac agagcctctg tttccgattc tgccaaactg cagatactca
     2521 ctcaggtgat ccatgaagaa gagagattta tcggaaatca gatattctat cagaatccgg
     2581 acaattcttt tttgacaaag gtactcaaga caaaactggg aatcccaatc actctatcca
     2641 tcatatatat tctgatcggt ctcaggctgg aacttcctct ttttggaatc aatatgcccc
     2701 ttcattttct ggtatcctat gagaatgatg attttcagac ctatgtggat cctttcaacg
     2761 gcggagtctg gatagacaag gatacctgcc tgcgatttct ggaagcaaac ggatattcca
     2821 cagagacaga tatgtttcct gttgcaagca cttcgtccat catcagaaga atgtacaata
     2881 atctgattct gatctacagg aatcagaacg aaaaaactct ggaaaatatt ctgatcagac
     2941 aagcttccat ccttcagcct gcataagcga aattttcata aggagtacca aagtgctgaa
     3001 aaattctcaa atgctttttt tcattaaaac ttctttcctg atcatttttc tgttctgctt
     3061 cggagtgttt tcacagacga acgatccctc agatcctaaa aattcaatta agaatctcac
     3121 ccagactcat acagtcgtga acactgtcga tcttactgca aattccaaga agatacttgc
     3181 aataggcgcg gattccaagc agatagaata caaactcacc ggtgaaaccg tatctactct
     3241 gaaagaattc ggcaatatgg gacttgtcat tacggataaa agcatacttg cagtttcagt
     3301 gaacgcttcc gagtttgcaa gacagccttt tttcggtgat gtaaatatta attatactgt
     3361 atctaattct attatttttg tatccacaaa ccggaaagta tacatatact cagtttcttc
     3421 ctccgaatgg aagactgtgg atattgcagg agaatatgtc agaacccagg ctgcattcga
     3481 caggacaggc actctgatta caaataaaag aatcatcgcc tattctgatt atataaaaga
     3541 tgtccgttcc tatcccatca acaatatggt gccggtatac agttgtacag taatggataa
     3601 taagattgta tatgacagta ttcagaaaac aatcatattc agagcggccg caggcgattt
     3661 tactgtaaac agttttaact gatttcctga agcttttttc tgagttttca tctggataga
     3721 ggttgggcta tccgatttct ggatccctgg ttaatgaagg atataaagta tcatattata
     3781 ttttttattg taatttatta attatttttg attcgacacg atgctttgct tttttgaaaa
     3841 attgagttca gtttatactc atattcaaaa gtctttgcgt atttctactt tagtgagcct
     3901 gtaaaatcaa ggttttcgaa tgtaaaaagc gcaattactt ttggtaatca gtttatttct
     3961 tttattgtcc gaaggattga ttgcgagact caggatttcc agagagataa tatttgctta
     4021 tctttcatgc tcaatgggtt ttggtattac tcaaatttct aatgaaattt tcatagagtc
     4081 aattgattat tgattgaaat tatattttcc gatgactaa
//
LOCUS       DAOQGJ010000091         4083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_11972_length_4083_cov_3.384558, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000091 DAOQGJ010000000
VERSION     DAOQGJ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11972_length_4083_cov_3.384558"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PK683_02290"
     CDS             <1..1252
                     /locus_tag="PK683_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH07304.1"
                     /translation="AGINFKQAKAVSIHPEGSETNPNPFVRIEYTSPSKQGTAEDVEY
                     DFLINATGPRLNFGATEGLGPEKNSHSVCTFGHAESAWKALRVSIEKMKKGETQTLLI
                     GTGHGNCTCQGAAFEYLFNVEHTLREHNVRDKANLVWISNEYELGDFGIGGMHIKRGG
                     YVTHSRIFAESLYAERGISWIKRAHVQNVGAGFVRYETLDGEEKEQKFDFAMLLPPFS
                     GVGLKAYNKKSEDISSILFAPSGFMYVDGDYSAKPYEEWKPEDWPNHYQSPAYKNIFA
                     IGIAFAPPHAISKPMKSPKGTPIFPAPPRTGMPSGVMGREAAKSILDMINGAEKPTRT
                     ASLARMGAACIASAGASLFGGSAVSMTMYPIVPDYKKFPLYGRDLDLTTGEIGLAGHW
                     IKYILHYVFMYKAKGNPLWKWIPE"
     gene            1267..1413
                     /locus_tag="PK683_02295"
     CDS             1267..1413
                     /locus_tag="PK683_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07305.1"
                     /translation="MKEIAPYSDSYFPPHPTEFTVKSRQNLLYQFYRFLVINLKMLKM
                     TRYH"
     gene            complement(1470..2099)
                     /locus_tag="PK683_02300"
     CDS             complement(1470..2099)
                     /locus_tag="PK683_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNH07306.1"
                     /translation="MDRKEFFRQGFLGLKKTLKQVDKIKEEAVSLASENLKKENEEEI
                     LSRSEPEITKSRPVRKGLKFPPGAVPKEKFFSLCTGCGDCISACPHETILPVFDSKQN
                     QNFPYVDPNFRPCHSCKDTPCISACETKALKPLKKKTAPKFGKAKIKFEFCLNSLYGE
                     KVCSRCEEICNIPNAIKIKKIKPSISASCTGCGLCAAACPSFPKAVLVK"
     gene            complement(2178..3206)
                     /locus_tag="PK683_02305"
     CDS             complement(2178..3206)
                     /locus_tag="PK683_02305"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNH07307.1"
                     /translation="MRKAFITGVLGQDGAYLSRLLLEKGYRVFGGARNFSDESVWRLK
                     KLGIENSIEKVLLDMADPKLIDSILEETMPDEIYNLAAQSSVAKSFEDPVGTAVVNAL
                     GPLYILEAVRKHCPSGRLFHAGSTEMFGHCPDIKQNEDSAFSPLNPYSSAKVYAYNIM
                     KNYRDIFGLFTVNGILFNHESELRGKQFFTSKVVNHAARYSSGELCILNVGNIDAERD
                     IGFAEDFVKAMFLSLQSDNPDDYIISAGKKYKIREFIEAAYRAAGTEIIWKGSGKEEK
                     GYDSSTGKLIIQVNPANFRPVDVESQWGDPSKIERLLGWKAVTELPEIADRMVKDEIH
                     SLKSEKPF"
     gene            3264..>4083
                     /gene="sppA"
                     /locus_tag="PK683_02310"
     CDS             3264..>4083
                     /gene="sppA"
                     /locus_tag="PK683_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNH07308.1"
                     /translation="MERNKLALLFILIFTVLSTIIGLFNITIQSSAGKSSTKLSHSKK
                     ILKEGDASALLLKIEGEIHSGKSTYSSTGADTVLARLREIEDRQDIKGILIEVNSPGG
                     TVAASQEIYEELMLLRKTKKIVVSMKDLAASGGYYIAAAADYIFAESGTITGSIGVIT
                     MTPDISGLLDKYNVKMNVYKQGKYKDILSMYKAPSEEEEEIIQNMLKDTYTRFISDVS
                     RGRNIPKADVEKSAEGKIFSGEAALKNHLVDAIGGRREARAKLSELAGSDGLLHL"
BASE COUNT         1128 a          878 c          928 g         1149 t
ORIGIN      
        1 ggccggaata aacttcaagc aggcaaaggc agtgtcgatt catccggaag gatccgagac
       61 aaatccaaat ccctttgtga gaattgaata cacttcaccc tcaaagcagg gaactgcaga
      121 ggacgtggag tatgattttc tcatcaatgc caccggccca aggctcaatt ttggagccac
      181 cgagggtctg ggtccggaaa aaaacagcca ctctgtatgc acttttggac atgcagaatc
      241 tgcctggaaa gcacttcggg tgtcaataga gaaaatgaaa aagggagaaa ctcagaccct
      301 tctgatcgga accggtcatg ggaactgcac atgtcaggga gcagcttttg aatatctgtt
      361 caacgtggag catactcttc gggaacacaa tgtcagagac aaggccaatc tggtctggat
      421 ttccaatgaa tacgaacttg gagattttgg aatcggagga atgcatatta aaagaggcgg
      481 ctatgtcact cacagcagaa ttttcgctga gtcactgtat gcggaaagag gaatttcctg
      541 gatcaaaaga gcgcacgtgc agaatgtagg agctggtttt gtccggtatg aaaccctgga
      601 cggtgaggaa aaagagcaga aatttgactt cgcaatgctc cttcctccct tttccggagt
      661 tggtttgaag gcttataata aaaagtctga agatatcagt tcgatacttt ttgcacccag
      721 cggttttatg tatgtggatg gagactacag tgcaaagccc tatgaggaat ggaagccaga
      781 ggactggccg aatcattatc agagtcccgc ctataagaat atctttgcaa tcggaattgc
      841 atttgctcct ccccatgcaa tttccaaacc gatgaaaagt cctaaaggaa ctccgatttt
      901 tcctgcaccg cccagaacag gaatgccttc gggtgtgatg ggaagagaag cggcaaaaag
      961 catactggat atgatcaacg gtgcggagaa gccgactaga acagcttctc ttgcaaggat
     1021 gggagctgcc tgcattgcat cagcgggagc gagtctgttc ggcggaagtg ctgtttccat
     1081 gacaatgtat cccattgtgc ccgattataa aaaatttcct ctctacggaa gagatctgga
     1141 tcttacaacc ggagagatcg gtcttgcagg tcactggatt aaatatattc ttcattatgt
     1201 atttatgtac aaagcaaagg gaaatccgct ctggaaatgg attcccgagt aaaaaggaga
     1261 aagattatga aagaaattgc gccctactca gacagttatt tcccgccgca tcccactgag
     1321 tttacagtga aaagcaggca gaatctgctc taccagtttt acagattcct cgtgatcaat
     1381 ctgaaaatgc tcaaaatgac aaggtatcac tgaacgtccg gcatgctgcg caatctgacc
     1441 tgtcctggaa gacgggcagg cggtctgcgt tatttgacga gaactgcctt tggaaagctg
     1501 ggacaggcgg ccgcacagag tccgcaccct gtacaggatg cactgatcga aggttttatt
     1561 ttttttatct ttatcgcatt tgggatattg catatttctt cacatctgct gcagactttt
     1621 tcgccgtaaa gtgagttcag acagaattca aatttgattt ttgcctttcc aaattttgga
     1681 gctgtctttt ttttgagcgg cttcagtgct tttgtttcac atgcggaaat gcagggagta
     1741 tccttgcagg aatggcaggg cctgaagttc ggatctacgt aaggaaagtt ctgattctgt
     1801 tttgagtcga atactggaag aattgtttcg tgagggcagg ctgaaatgca gtccccgcat
     1861 ccagtgcaga gactgaagaa tttttccttc ggtacggctc cgggagggaa tttcaaacct
     1921 tttctgacag gcctgctttt tgtgatctca ggttctgatc tggagagaat ctcttcttca
     1981 ttttctttct tcaggttttc tgaagcaaga gaaactgctt cttccttgat tttatccacc
     2041 tgtttcagtg tttttttcag tcctagaaaa ccctgtctga aaaactcttt cctgtccaaa
     2101 cattttcctc ctgatctgtt acagttttcc agccgttttc ctttagtaaa gtaaaatgaa
     2161 aatagggaaa gaaagattca gaagggtttt tccgatttca aggaatggat ctcatccttc
     2221 accattctgt ctgcaatttc aggaagctct gtgactgcct tccagccgag aagtctttct
     2281 atcttggaag gatctcccca ttggctttcc acatccaccg gtctgaagtt tgccgggttg
     2341 acctgaataa tcagttttcc tgtcgaggag tcgtatcctt tttcttcctt tccgcttcct
     2401 ttccaaatga tttccgttcc tgcggcacgg taagcagcct ctatgaattc tctaatcttg
     2461 tattttttcc ctgccgaaat aatgtaatca tcaggattat ctgactgcag agaaagaaac
     2521 attgccttta caaagtcttc tgcaaatcct atatcccttt cagcatctat attgcctaca
     2581 ttcagaatgc ataattctcc tgaagaatac ctggctgcat gatttacgac cttgcttgta
     2641 aaaaactgct tgcctctcag ttcagattca tgattgaaca gtattccgtt tacagtgaac
     2701 agtccgaata tatctctgta atttttcatt atattgtatg catatacttt tgcggaagag
     2761 taggggttca gaggagaaaa tgccgagtcc tcattctgtt ttatatccgg gcaatgtccg
     2821 aacatctcag tgcttcccgc atgaaaaagt cttcctgaag gacagtgttt tctgactgct
     2881 tccagtatgt acagcgggcc taaggcattc acaaccgcag tgcctaccgg atcttcaaaa
     2941 ctttttgcta cactgctctg ggctgcaaga ttgtatattt cgtcaggcat tgtttcttcc
     3001 agaattgaat cgatcagctt cggatcagcc atatccagca ggactttttc tatgctgttt
     3061 tcaatgccca gttttttcag tctccataca gattcatccg agaaatttct ggccccgccg
     3121 aatacccgat atcccttttc cagaagaagt ctggaaagat aggctccgtc ctgacctaaa
     3181 acgcctgtaa taaatgcttt tctcattttc cggcagaatt tttattgtgt atgatatgat
     3241 tcaaaatctc aaagagagtt ctaatggaaa gaaataaatt agccctgctg ttcatactga
     3301 tattcactgt actgtccaca atcattggtc tcttcaatat taccatccaa tcgagtgccg
     3361 gaaagtcctc aaccaaactt tctcattcca aaaaaatact gaaggaaggg gatgcaagtg
     3421 cacttcttct caagattgaa ggggaaattc attccggaaa atccacctat tccagtacag
     3481 gtgcagatac agtgcttgcg aggctcagag agattgaaga caggcaggac atcaagggaa
     3541 tcctgattga ggtgaattct cccggcggta cggttgcagc ttcccaggaa atctatgaag
     3601 agctgatgct tctccgcaag acaaaaaaaa tagttgtgtc tatgaaggat ctggcggctt
     3661 cagggggata ttatattgca gcagctgctg actatatttt tgccgagtca ggaactatca
     3721 ctggatccat aggcgtgatt acaatgacgc cggacatctc aggacttctc gataaataca
     3781 atgtaaagat gaatgtctat aaacagggga aatacaagga tattctgtcc atgtacaagg
     3841 caccgagtga agaagaggaa gaaatcattc agaatatgct taaggatact tacacgcgtt
     3901 ttatcagcga tgtgagcagg ggaagaaaca tccctaaagc agatgtggag aaatccgcag
     3961 aaggaaagat tttttcaggg gaggctgctt tgaaaaatca tctggtggat gcgatcggcg
     4021 gaagaagaga agcaagggca aaactttcag agctcgcagg tagcgacgga cttcttcatc
     4081 tga
//
LOCUS       DAOQGJ010000092         4065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12066_length_4065_cov_3.536658, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000092 DAOQGJ010000000
VERSION     DAOQGJ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12066_length_4065_cov_3.536658"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PK683_02315"
     CDS             complement(<1..943)
                     /locus_tag="PK683_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HNH07309.1"
                     /translation="MKIKDSKIAIGLGEVKGAKYKVVHIIKDDITPGQTKALKSVMDA
                     KIFAGETGQVYTDTAENTIYLGLGDRKKLNIRRIASVYLKFAEQSVKWKNTGLEIEVP
                     KSLTDRFSPFLLAYQIANSFAISAFPVDILSKAYREREISPGPVNFLFEDSSAEAEAK
                     RAIGKSKIVTYYLNGAREIEHLPANHFTPEEFVLRSKEIAKEAKLKITVFDEDRLKKE
                     KFGGILSVAAGSDKKPKMIILEYSPKNPVTKKKLAIVGKGLTFDSGGISIKPSAEMHE
                     MKYDMCGAAAAIHAIGAISKLGIRIPVIAAIGVVENMP"
     gene            complement(940..1416)
                     /gene="bcp"
                     /locus_tag="PK683_02320"
     CDS             complement(940..1416)
                     /gene="bcp"
                     /locus_tag="PK683_02320"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNH07310.1"
                     /translation="MADTLEGKKAPSFQALNTKGEKVSLKNLAGEKGLVLYFYPKDST
                     PGCTQEACDFRDSLASLKKLGVNVVGVSKDDQKSHQKFTEKQSLNFDLLSDTEGEICE
                     KYGVWQLKKFMGKEFMGIVRSSFLISSDLKILKSYRNVKVKGHVEEIKKDIKELLK"
     gene            complement(1472..2965)
                     /locus_tag="PK683_02325"
     CDS             complement(1472..2965)
                     /locus_tag="PK683_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH07311.1"
                     /translation="MKAKKKTAVAKKKEKKAVITEIPQTGISEMKAEVERIFSLQMQN
                     RFHIAGTTAKERIQKLKRILHWIEKHRSEIYSALKKDFRKPETETDISEILVALTEIK
                     HAVSHLKKWMHPQRVDKTLSFVTASGWVQYEPRGVVLIISPWNFPFNLTLGPMISAIA
                     AGNCIMLKPSEYTPATSALIRKMITDIFPENEVAMFEGDKDVSSALLEKPFHHIFFTG
                     SPEVGKLVMAAAAKNLSTVTLELGGKSPVIIDEDADMNDAVEKITWGKFMNNGQTCIA
                     PDYALVHKKRHTEFLEKMQKTVQKFYGASESDRKASPHFARIISARHHARLQKMIEDS
                     KKEGAKIEYGGIYDADDLYISPTLLSHVTLDMPIMKEEIFGPVLPVLAVDSIDHAVKV
                     VQKMEKPLALYIFSRNRTNVHKVLKETSSGGTCINETLIHFLHPNLPFGGVNYSGAGN
                     SHGFYGFRAFSHERAILRHIKHSPLKLLYPPYTEKVKKLASLVLRYF"
     gene            complement(3174..3977)
                     /locus_tag="PK683_02330"
     CDS             complement(3174..3977)
                     /locus_tag="PK683_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNH07312.1"
                     /translation="MKKQSLTLNKLLIILFLLNCASVQAKEDFGAKSILIEDRKSGKV
                     IFKKNEKEIYPIASLTKLVTAVTASRIFENNDLIQIKKSHLPKNSLESKADLKAGKEY
                     SFTEILHGLLIPSGNDAARGIEDAMLKRGKSFAEETADFLKKENLSSTSIFEAVGLNS
                     ENRSNAEDLSRIADITFGNPLLSEIMTKSEYRIKDTKGSTHLIRNRLKLNSYRDWTIY
                     GKTGRTKKAGQCFAGIIEKNTKKYKIIFLGSENAEAEIKSAADFLERRE"
     gene            complement(3949..4026)
                     /locus_tag="PK683_02335"
     CDS             complement(3949..4026)
                     /locus_tag="PK683_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07313.1"
                     /translation="MGLENIEDITADIDQALEKTKSDTE"
BASE COUNT          944 a          923 c          825 g         1373 t
ORIGIN      
        1 ccggcatatt ttcgaccacg cctatcgcag caatcacagg aattctgatt ccaagttttg
       61 agattgcacc gattgcatga atcgccgcag ctgcaccgca catatcgtat ttcatttcat
      121 gcatttcagc acttggcttg atgctgattc cgcctgaatc gaacgtcagt ccttttccga
      181 caatagccag tttcttttta gttaccggat ttttcggaga gtactccaga atgatcattt
      241 tcggtttttt gtccgatccg gctgcaacgg aaagaattcc tccgaatttt tccttcttga
      301 gtctgtcttc atcgaatacg gtgattttca gttttgcctc ttttgcaatt tccttcgatc
      361 tcagtacaaa ctcttccgga gtgaaatgat ttgcaggaag atgttctatt tctcttgcac
      421 cgttaagata atatgtgaca attttggact tgccaattgc tcttttggcc tcagcctcag
      481 ctgaactgtc ttcaaaaagg aaattgacgg gaccgggaga gatttctctt tcccggtaag
      541 ccttgctcag tatgtccaca ggaaaagcag aaatggcaaa tgagttggca atctggtaag
      601 ccagaagaaa cggtgagaat ctgtcggtca gagatttggg tacttctatc tcgagtccag
      661 tgtttttcca ttttacggac tgttcagcaa acttcagata aaccgatgca atccttctga
      721 tgttcagttt ttttctgtcg cccagaccga gatatattgt attctctgcg gtatcggtat
      781 aaacctgtcc tgtttcccct gcaaaaatct ttgcatccat tacagatttc agagccttcg
      841 tctgacccgg ggtaatatca tcttttatta tatgtactac tttgtatttt gcgcccttga
      901 cttcaccgag gcctatcgca atttttgaat ctttgatctt catttcaaaa gttccttaat
      961 gtcctttttt atttcttcca catgaccttt cacttttacg tttctgtatg acttcagtat
     1021 tttcaggtca gaactgatga gaaaactgga gcgcacgatt cccataaatt cttttcccat
     1081 aaattttttc agctgccata ccccgtattt ttcacagatt tctccctctg tgtccgagag
     1141 cagatcgaaa ttcagcgact gcttttctgt aaacttctga tgggatttct gatcatcctt
     1201 ggatactccg actacattga cgccaagttt tttcagactt gccagactgt ctctgaaatc
     1261 acatgcctcc tgagtgcatc ccggtgtgct gtccttcgga tagaaataca ggacaagtcc
     1321 tttctcgcct gcaagatttt tcagggatac tttttctccc ttcgtgttta aagcctgaaa
     1381 tgagggcgct tttttccctt caagcgtgtc agccattctg tcctcctgat acagtcataa
     1441 ttcttaaaaa ccctctgtgg acaagcattg ttcagaaata cctgagcaca agggatgcca
     1501 attttttgac cttttcagtg taaggagggt aaagaagttt cagaggagaa tgttttatat
     1561 gacggagaat tgcgcgctca tgagagaatg ctctgaaccc gtaaaagccg tgagaattgc
     1621 ctgcgccgct gtaattgact ccgccgaaag gaagattagg atgcagaaag tgaatcagtg
     1681 tttcattgat gcaggttcct cctgacgatg tttccttcag aaccttgtgc acattcgttc
     1741 tgtttctgct gaatatgtaa agagcaaggg gcttctccat tttctgcaca accttgactg
     1801 catgatcaat ggagtctact gcgagcacag gcagaacagg tccaaagatt tcttctttca
     1861 tgatcggcat atcgagagtg acgtgagaaa gaagggtggg ggagatgtac agatcgtctg
     1921 catcgtaaat tcctccatat tcaattttgg ccccttcctt ctttgagtct tcgatcattt
     1981 tctgcagtct tgcgtgatga cgtgcactga tgattctcgc aaaatgagga cttgcctttc
     2041 tgtcggactc ggaagctccg taaaatttct gaacagtctt ctgcattttc tccagaaatt
     2101 cagtatgcct ttttttatgt acaagtgcat agtctggtgc aatacaggtc tgtccgttgt
     2161 tcataaattt tccccaggtg attttctcca cagcatcatt catatcagca tcctcatcaa
     2221 tgatgactgg agattttcct cccagttcaa gagtcactgt gcttaagttt tttgctgcag
     2281 ccgccatcac gagttttccc acttccggac ttcctgtgaa gaagatatgg tggaaaggtt
     2341 tttccagtag cgcacttgaa acatctttgt ctccctcaaa cattgcgacc tcattttccg
     2401 ggaaaatatc agtgatcatt tttctgatca gggccgacgt cgcaggagtg tactcggaag
     2461 gtttcagcat aatgcagttt cctgctgcga tggccgatat catcggccca agagtcagat
     2521 tgaaaggaaa attccagggt gatatgatga ggacaactcc tctcggttcg tactgcaccc
     2581 agccggaagc tgtcacaaag gaaagagtct tgtccactct ctgcgggtgc atccattttt
     2641 tcagatgact caccgcgtgt tttatttcag tcagtgcgac cagtatttcg gaaatgtccg
     2701 tctctgtttc aggctttcgg aaatctttct tgagtgcaga ataaatttct gatctgtgtt
     2761 tttcgatcca gtgcagaatt cttttcagct tttgaatcct ttcttttgct gtcgtgcctg
     2821 caatatgaaa tctgttctgc atctgcaggg agaaaattct ttccacctct gccttcattt
     2881 ccgaaattcc agtctgggga atttcagtaa ttacagcttt tttttccttt tttttggcga
     2941 ctgcggtctt tttctttgct ttcataagaa tctccttccg aatacctgat accgtttctc
     3001 tgctgattca gtatctgaag gttttaaaat cagtcaatca gatattgact gactcacctt
     3061 acgccttcgg ctgcaggcgg ggcgttcaat ttaagaagtc ccattttgcg gtttcaaatt
     3121 tattgcacag ggatctgaaa tcctgcagtt taagtattct tcgtaatcct gcatcattct
     3181 cttctctcca gaaaatcagc tgccgatttg atttcagctt ctgcattttc tgaacctaga
     3241 aaaatgattt tatatttttt tgtattcttt tctatgatcc cggcgaagca ttgacccgct
     3301 ttttttgttc tgcctgtttt tccgtaaatt gtccagtctc tgtaagagtt cagcttcagc
     3361 ctgtttcgga tcagatgagt gcttcctttt gtgtctttga ttctgtattc ggattttgtc
     3421 ataatttccg aaagcagagg atttccgaat gtgatgtctg cgatccggga aaggtcttct
     3481 gcattcgatc tgttttctga attcaggccg actgcttcaa aaatggaagt ggagctgaga
     3541 ttttccttct tcagaaaatc tgctgtctcc tctgcaaagg actttccgcg tttcagcatc
     3601 gcatcttcaa ttcctcttgc cgcatcattt cccgacggaa tcagaagccc gtgcagaatt
     3661 tcggtgaaac tgtattcttt tcctgctttc agatccgcct tcgattcgag tgaattttta
     3721 gggagatggg atttttttat ctgtatgagg tcgttgtttt caaatattcg ggaagccgtg
     3781 actgcagtga ctagttttgt caggcttgca ataggatata tttccttttc atttttttta
     3841 aatatgactt ttcccgattt tctgtcttca atcagaatgg actttgcccc gaaatcctcc
     3901 tttgcctgaa ccgaagcaca gttcagtaga aagaggatga tcagcagttt attcagtgtc
     3961 agactttgtt ttttcaagag cctgatcaat atccgcagtg atgtcttcaa tgttttcaag
     4021 acccacggag acccttatca gtccaagctc aatgcctaca gcttc
//
LOCUS       DAOQGJ010000093         4053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12128_length_4053_cov_2.908454, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000093 DAOQGJ010000000
VERSION     DAOQGJ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12128_length_4053_cov_2.908454"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PK683_02340"
     CDS             complement(<1..475)
                     /locus_tag="PK683_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNH07314.1"
                     /translation="MKIFLIGIGGIAMGNLARMLQELGHEISGSDHDLYPPMSDQLAD
                     WGMKVYSGFKAANVKGSDLVIIGNAISRGNPEVEEVLNSGMDYISMPEAISKFILKGR
                     KVIVIAGTHGKTTTTFLLHHLLKEAGLSPGLFAGGIRKDGHAGFELGKGEYFVIEG"
     gene            complement(478..1110)
                     /locus_tag="PK683_02345"
     CDS             complement(478..1110)
                     /locus_tag="PK683_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07315.1"
                     /translation="MNFVSVSKNLGIMEDDYKGIFGDYERLLREIFLSPMDSFDFYLN
                     SGKFSLLHLFTVHILLWTLCPLSILIQKASVLLMNFRIREEIIDSWTWKDYSVVLIYP
                     AAMLLASIMEGFRNAYNETRQKTVAREKNLVYLAFLPFSATGVFWILPKPFPFFFLVF
                     ALYYSFRILSKTLKNLSGYTDQEYRIYLMYILILFSLVSLGAASILKTVR"
     gene            1186..2958
                     /locus_tag="PK683_02350"
     CDS             1186..2958
                     /locus_tag="PK683_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNH07316.1"
                     /translation="MDSILNIRQIPKSKFDRIVTYWLSFLCFASPFIGLGVFFPIGVL
                     FLYRSNHTLRASAFQSAALQIFVYAVSFPLDFICLWDETSPYCIAGLKNERALAFQGY
                     FLLFWAVVGFIILFLEARFTVSKYGYLKSSKYRYSSERSASGDSFLYTFFIVFLSWFS
                     AVFLFVMLTALYLFLSAKDSQMELDSLIALSFKGEGSLLFWLLNVHTVSKMVGRKTVL
                     WVLRRPYLYLYANTRLGSSSTVPETASWQRKSRYAKFREAVMPGLGQVYLNRYWPGFS
                     TLFVYLLFLLFASTAVIYHLDYVFGTKFLQFFGLKPGIPDKEFIRYADNIFFPIFCTM
                     CLISVYAFAQFLVRHFLKREAEPIEERGLRSGFQDNLALSVLLHLTLLSVMFIIPVTV
                     QRQSSSQKKRDMSKDHFQPEKMEFYFIDPEIPDDVKDLNGGVVSGTETPTETEGMKIP
                     DEEIKDEGKVKGYVKKIKGKKLPKTYSNYISARMRGPEMYMDYWKRAPHPYSCVVAYT
                     ITTEGEITDVYIVEPSGYPDQDRLTVELVQSMSPVMPPPGVKGDVRVTELFWNGPIDP
                     DAMPTELQKEMVSMFDGRYMEEEF"
     gene            complement(2965..3726)
                     /gene="rlmB"
                     /locus_tag="PK683_02355"
     CDS             complement(2965..3726)
                     /gene="rlmB"
                     /locus_tag="PK683_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNH07317.1"
                     /translation="MEKKISQAVFGKRNCEELLKRLLKEGRPVSEWPYREFLIKKNPS
                     KDLVSLLEKIPEEIHVRHVSIGDFDSMFPGMNHQGIAFLKKSGSSSFEYSDIEKFFSE
                     ENHEKPVLILDRIQDVGNFGSIIRTAECLGVKHIIIPERDSAMVNDTVMKTSSGAVHH
                     VNIYRVTNLSQILDRLKENGFWIASASDRGSESWEEISGDHLVLILGSEGEGVKRILM
                     EKSDYNIRIPMHGKISSMNVSVACGILLDRIVNRS"
     gene            complement(3711..>4053)
                     /locus_tag="PK683_02360"
     CDS             complement(3711..>4053)
                     /locus_tag="PK683_02360"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNH07318.1"
                     /translation="LAGLADDLNVPKSLSSVFEFVKHMNADLDSEKLGREECMDSVRF
                     LFQVNRLLDVLEFSKKDGSADEEIDRLVAARIEARKNKDFKKADEIRDELNRLGIILE
                     DTKTGVKWKRK"
BASE COUNT         1118 a          929 c          812 g         1194 t
ORIGIN      
        1 ccccttctat tacaaaatat tctcccttgc cgagttcaaa tcctgcatga ccgtcctttc
       61 gtattccccc tgcaaaaagc ccgggagaaa gtcctgcttc cttcagaaga tggtgcaaaa
      121 gaaatgtcgt tgtggtcttt ccgtgagtcc ctgcaatgac gatcaccttt ctgcccttca
      181 ggatgaattt ggaaatagct tcaggcatac ttatatagtc cattcccgaa ttcaaaactt
      241 cttccacttc aggatttcct ctggatatcg cgtttcctat aattacaaga tcggatcctt
      301 tgacatttgc agccttgaat cctgaatata ctttcattcc ccagtccgcc agctgatcgg
      361 acataggagg atacagatca tggtctgagc cggaaatttc atgaccgagc tcctgaagca
      421 ttctggcaag attgcccata gcaatcccgc cgattccgat taaaaatatt ttcatgacta
      481 cctgactgtt tttaagattg aggctgcccc cagactgacg agagaaaaaa ggatgagtat
      541 atacataaga tatatcctgt attcctgatc agtgtaccct gaaagatttt tcagcgtttt
      601 ggaaagaatg cggaaagaat agtacagtgc aaaaacaagg aaaaagaacg ggaacggctt
      661 aggaagaatc cagaaaacac cggtggcgct gaaaggcaga aatgccagat agaccagatt
      721 tttttccctt gccacagtct tctgcctggt ttcgttgtat gcatttctga aaccttccat
      781 aatggatgca agaagcattg ctgcgggata aatcagtacg accgaatagt ctttccaggt
      841 ccaggaatca atgatttcct ctctgattct gaaattcata agaaggacag aagctttctg
      901 aatcagtatg gacaggggac agagagtcca gagaagaata tgcactgtaa agagatgaag
      961 caggctgaat ttcccgctgt tcagataaaa gtcaaatgag tccatcgggc ttaagaatat
     1021 ctccctgagg agcctctcgt aatcgccgaa aattcctttg taatcgtctt ccattatacc
     1081 cagattttta ctgactgaaa caaaattcaa tgcctttatt cagaaagaca aagtcgaaaa
     1141 agatttttct cttgatcata ctgcacaagt ctaaagctta gaatcatgga cagcatactc
     1201 aacatcagac agatcccaaa atcaaaattt gacagaattg tcacttactg gctctccttt
     1261 ctctgttttg ccagtccttt catcggcctt ggggttttct ttccgatcgg tgtactgttt
     1321 ctttacagat ccaatcatac tctccgggca tctgcatttc agtccgctgc tcttcagatt
     1381 tttgtatatg cagtctcctt ccctctggac ttcatctgtc tctgggatga aacgtctcca
     1441 tactgcattg ccggtctgaa gaatgaaaga gcacttgcat tccaggggta ttttcttctt
     1501 ttctgggcag tcgtcggatt catcatactt ttcctcgaag ccaggttcac tgtttcaaaa
     1561 tacggatatc tgaaaagctc caaatacaga tattcctctg aaagaagtgc ttccggcgac
     1621 agttttcttt atacattctt catagtattc ctgtcctggt tttctgccgt atttcttttt
     1681 gtaatgctga ccgcactgta tcttttcctc tctgcaaaag attcccagat ggaactggac
     1741 agtctgattg cactctcttt caaaggagag ggttcccttc ttttctggct tctcaatgtt
     1801 catacggtct caaaaatggt cggaagaaag actgttcttt gggttctgag aaggccttac
     1861 ctgtaccttt atgcaaacac cagactaggc tcctcttcca cagttccgga aacagcttcc
     1921 tggcagagaa agtccagata cgcaaaattc agggaagccg taatgccggg actgggacag
     1981 gtgtacctga acagatactg gccgggattc tccactcttt tcgtatatct tctttttctt
     2041 ctttttgcat ccacagcagt catataccat cttgattatg tgttcggaac caagtttctt
     2101 cagtttttcg gactgaagcc gggaataccc gacaaggaat tcataagata tgcagataat
     2161 atattttttc caattttctg cacaatgtgt ctgatttctg tctatgcatt tgcacagttt
     2221 ctagtcagac attttctgaa aagagaggca gaaccgattg aggaaagagg acttcgatcc
     2281 ggatttcagg acaatcttgc actgagcgta cttctccacc tgacacttct ttcagtgatg
     2341 ttcattattc ctgtcacagt gcagagacag tcctcctctc agaagaaaag agatatgtcc
     2401 aaggatcatt ttcagccgga aaaaatggaa ttctatttta ttgatccgga aatccctgat
     2461 gatgtaaagg atctgaacgg cggtgtagtt tccggtacag aaacacctac agaaactgaa
     2521 ggaatgaaaa ttccggatga ggaaatcaag gatgaaggaa aagtaaaagg ttatgtaaaa
     2581 aagatcaaag gcaaaaaact tcccaagaca tattccaatt atatttcagc cagaatgaga
     2641 ggacctgaaa tgtacatgga ttactggaaa agagcgcctc atccttattc ctgcgttgtc
     2701 gcttatacaa ttacaacgga aggagaaatc acggatgtct acatagtgga accttccgga
     2761 tatcctgatc aggacagact caccgtggag cttgtgcaga gtatgagtcc tgtaatgcct
     2821 cctcccggag taaaaggaga tgtcagggtg acggaacttt tctggaacgg tccgatcgat
     2881 ccggatgcaa tgcctactga actgcagaag gaaatggtaa gcatgtttga cggaagatat
     2941 atggaagagg agttttaaag cgagctacga tctgttcact atcctgtcga gaaggattcc
     3001 gcaggctacg gaaacattca tggatgaaat tttcccgtgc atcggaatcc ttatattata
     3061 gtcggatttt tccatgagta tcctcttgac accttcccct tcgcttccga ggatcagaac
     3121 aagatggtcc cctgaaattt cttcccagga ttcagatccc cgatcgcttg cagatgcaat
     3181 ccagaatccg ttttccttca gtctgtccag aatctgagac aggtttgtga cacggtatat
     3241 atttacatga tgaactgcac cggatgatgt cttcattaca gtatcattaa ccattgcaga
     3301 atccctttcc ggaataatga tatgcttcac tccgagacat tccgccgtcc tgatgatact
     3361 tccgaaattt cccacatcct gaatccggtc cagaatcaga acaggttttt catgattctc
     3421 ttcagagaaa aatttctcta tatcggaata ttcaaaggaa gaagatcctg attttttcag
     3481 gaatgcaatc ccctgatgat tcatcccggg aaacatggaa tcaaagtcac ctatggaaac
     3541 atgtctgaca tgaatctctt cagggatctt ttccaggaga gaaaccagat cctttgaagg
     3601 attctttttg atcagaaatt ctctgtaagg ccattcagaa accggtcttc cttccttcag
     3661 aagccttttc aggagttcct cacagtttct ttttccgaat actgcctgac ttattttctt
     3721 ttccatttca cgcccgtttt tgtgtcttcc agtatgattc ccagtctgtt cagctcgtcc
     3781 ctgatctcat ccgccttttt gaagtccttg ttttttcttg cttcaatcct cgcagcaacc
     3841 agtctgtcta tttcttcgtc tgcagagccg tctttttttg aaaattccag aacatccaga
     3901 agcctgttca cctgaaacaa aaacctgaca gaatccatac actcttctct gccgagcttt
     3961 tctgaatcca gatctgcatt catatgtttt acaaattcaa acacggagga gagagatttg
     4021 ggaacattca ggtcatcggc aagtccggcc aaa
//
LOCUS       DAOQGJ010000094         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12184_length_4040_cov_3.115182, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000094 DAOQGJ010000000
VERSION     DAOQGJ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12184_length_4040_cov_3.115182"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..3409
                     /locus_tag="PK683_02365"
     CDS             38..3409
                     /locus_tag="PK683_02365"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNH07319.1"
                     /translation="MKFKYYFEDVSSFSVPEYEDFIFSNGSVQEGRRDAHSYTDFEEY
                     SGEEESEHAAVPKKKNTIIKKELKLDRTGSAKFSIPEIPEKTSIQTLVTEMEYKDPNG
                     EIQTIMKGSSIYPSDILVGIRDEGWSSSEKVKAEAAVLNLKRQPMSNVDVEILIYKKE
                     NYSYRRRLVGGFYAYDNFSEVKPAGVFCSGKTNANGIFSCEKSIPVKGTVYLAAKAKG
                     KQGTDVYANKEIWIAGDDDYWFDAHNSDRMDIIPDRKEYRPGDTAKIQVKAPFQKFKA
                     LVTTEREGIMDAFIVDLTGKNPVAEVPVKGNHAPNVFVSVFAVRGRTADPKPTALVDL
                     GKPSYKLGITQLNVDRQKHELTVKVSSDRENYKARETVRAKVKVSPKSGSGRIEKGEI
                     AVAVVDEGLLDLKPNETWKVLENMLKTRSLTVQTSTAQMQVIGRRHFGRKAIAPGGGG
                     GKDSTRELFDTLVYWKSVISLNSEGEAEFSFPLNDSMTSFRIVALAGADSDKFGTGYT
                     SIKSSKDLMLISGLPTLMREGDTYYPEVTVRNTTASEKEVTVSGKLVKPEISLEEKKF
                     RLGAGESKEVFWKLDIPVSADKLTYEISAVSGNEQDKIRITQKVGASVPVSVIQGTLR
                     QVDREMEMPVEFPKDAVPGRGGIQVNLSPSLLDSVSGVKDYMRHYSYTCMEQEISKSI
                     SLEDRERWNRVIQKMPAYLDRDGLLKYFPNMEDGSVILTSYALSISNEAGLEIPEFSR
                     EKMTAALEGFVNGTLSRKSHLSAADLTLQKLSAIEALSRYGKAVPAMLEPLHFQVQLL
                     PTSSVLDYFNVLNRLEVSDRAAKIKSAEQVLLARMNMQGSTMKFTSEANDSLWWLMVS
                     PDVNAVRLILSMTGAGKWKDNMGRIMRGVLSRQKKGSWDITTANAWGVLAVKKFASEY
                     EREPVGGNTSVSLFDSTTSADWKEKPKGSVINAGWKEKPDTLKISHTGSGKPWAVIYS
                     RAAVPLKESVSNGYTVKKTMIPLEVKNPGKWTRGDICRVQLKVKADSDMTWVVISDPI
                     PAGASILTGGLRKTSAVEGEKTDYNVYPAYEERSYEAYRIYYEYVPEGEFTVEYTVRL
                     NQEGKFTVPQTRIEAMYSPDMNGETPNGQMDVSRE"
     gene            3413..>4040
                     /locus_tag="PK683_02370"
     CDS             3413..>4040
                     /locus_tag="PK683_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07320.1"
                     /translation="MMLFIGILCILQLLLTNWGLWVLLKIFRVKRVQTSGGFFIFCIP
                     LLIFYGRNIHIVVTEKKDSDSANLKIGSIFFALNPLKIFIGKIYLEHFHLYRTHLFYL
                     NRIPSKEKIKYLPPVGRVRIYIRRLENCSVDVEDRTVFPVYRNTVREIELKNGRMDAA
                     VPLTVIFFSDYGRCRIGSGDIVTEMRTDLHGMLKIQGITWGELINLEGI"
BASE COUNT         1232 a          811 c         1022 g          975 t
ORIGIN      
        1 aagctatttt tccggtggaa ctgcaggagg acttcctgtg aagttcaaat actattttga
       61 ggacgtttcc tctttttcag ttcctgaata tgaagatttt atcttcagca acggttccgt
      121 tcaggaagga agaagagatg ctcattccta caccgatttt gaagagtatt ccggtgaaga
      181 ggaatctgaa catgcagctg tcccgaagaa gaaaaataca atcattaaaa aggaactgaa
      241 actcgacaga acaggttctg caaaattcag cattcccgag attcccgaaa agacatccat
      301 tcagacgctt gtcacagaga tggaatacaa ggatccgaac ggagaaattc agacgatcat
      361 gaaaggaagc agtatttatc cttcggacat actggtcgga atcagagacg agggatggtc
      421 ctcatccgaa aaggtcaagg cagaggctgc ggtgctcaat ctgaaacgtc agccgatgtc
      481 aaacgtcgat gtggagattc tgatctataa aaaggaaaac tattcgtaca gaagaagact
      541 tgtgggcggt ttttatgcct atgacaattt ctctgaagta aagcctgcag gggtcttctg
      601 ttcaggaaag acaaatgcga acgggatatt ttcctgtgaa aaaagcatcc ccgtcaaagg
      661 aacagtgtat ctggctgcaa aggcaaaagg aaaacagggc actgatgttt atgcaaacaa
      721 ggagatctgg attgcaggag acgatgatta ctggtttgat gcgcacaaca gcgacaggat
      781 ggacatcatt cctgacagaa aggaatacag accgggagac acagcaaaga ttcaagtaaa
      841 ggctcctttt cagaaattca aggctcttgt cacaacggaa agagaaggca tcatggatgc
      901 attcattgtg gatctgaccg gaaaaaatcc tgtagcagag gttcctgtga agggaaatca
      961 cgcaccgaat gtatttgtat ccgtatttgc agtgcgggga aggacagctg atcctaagcc
     1021 tacggcactc gtggatctgg gaaaaccttc ctataagctc ggaatcactc agctgaatgt
     1081 ggacagacag aaacatgaac ttacggtaaa agtgagttct gacagagaaa actacaaggc
     1141 aagagaaacg gtgcgcgcaa aagtgaaggt ctctcctaag tctggttccg gcagaatcga
     1201 aaaaggagag attgctgttg cagtcgtgga tgagggactt cttgatctca agccgaatga
     1261 aacctggaag gttcttgaaa atatgctgaa gacaagaagt cttactgtgc agacatccac
     1321 tgctcagatg caggtgatcg gaagaagaca ttttggaaga aaggcaattg ctccgggagg
     1381 agggggcgga aaggattcca ccagagagct gttcgacact ctagtctact ggaaatccgt
     1441 catttctctg aattcagaag gagaggcaga gttcagtttt cctctgaatg attccatgac
     1501 atccttccgg attgttgctc ttgcgggagc tgattcagac aagttcggga cgggatatac
     1561 cagcattaaa tccagcaagg atttaatgct catttcaggg cttccgacac tgatgagaga
     1621 aggggataca tattatcctg aagtcaccgt cagaaataca actgcttccg agaaggaagt
     1681 gacagtttca ggaaaactgg tcaagcctga gatttctctg gaagagaaaa aattcagact
     1741 gggagcaggc gagtccaaag aggtgttctg gaaactcgat attcccgtat ctgctgataa
     1801 gcttacttat gaaatttccg ccgtctcagg aaatgaacag gataagatca ggatcactca
     1861 gaaagtaggt gcttccgttc cagtctcagt cattcaggga accctccgtc aggtggacag
     1921 ggaaatggaa atgcctgtag agtttccgaa ggatgcagtt ccaggcagag gcggaattca
     1981 ggtgaacctt tctccctcgc ttctggattc tgtttcaggc gtaaaggatt atatgagaca
     2041 ttactcatat acctgcatgg aacaggaaat atccaaatcc atttcactgg aagacaggga
     2101 gagatggaac agagtcattc agaaaatgcc tgcctatctg gaccgtgacg gtcttctgaa
     2161 gtatttcccg aatatggaag acggaagcgt gattctcact tcctatgcac tctccatttc
     2221 aaatgaggct gggcttgaga ttcccgagtt cagcagagaa aaaatgactg cggcactgga
     2281 aggattcgtg aacggaactt tgagcagaaa gagtcatctc agcgctgccg atctcacact
     2341 tcagaaactg agcgcaattg aagctttgag cagatacggc aaggctgttc ctgcaatgct
     2401 cgaacctctt cattttcagg tgcagcttct tcctacctct tccgtgctgg actatttcaa
     2461 tgttctgaac agacttgagg tgtccgacag ggctgcaaaa atcaagtctg cagagcaggt
     2521 actccttgcc agaatgaata tgcagggaag cacaatgaaa tttacttcag aagcaaacga
     2581 cagtctctgg tggctgatgg tatctcccga tgtaaatgca gtcaggctga ttctctccat
     2641 gacaggagcc ggaaaatgga aggacaatat gggaagaata atgagagggg ttctttccag
     2701 acagaaaaaa ggctcctggg atattacaac tgcaaatgca tggggagttc ttgccgtgaa
     2761 gaaatttgca tcagagtatg aaagggaacc agttggcggg aatacgtcgg tctctctgtt
     2821 cgacagtaca acgtcagctg actggaagga aaaaccgaag ggatcagtta tcaatgcagg
     2881 atggaaggaa aagccggata cattgaagat ttcccatacc ggttccggaa agccctgggc
     2941 tgtaatctac agcagggctg ccgtccctct gaaagagtcc gtatcaaacg gttatacggt
     3001 caaaaagaca atgattcctc tggaagtgaa gaatcccgga aaatggacaa ggggggacat
     3061 ctgcagagtc cagctgaaag tcaaggctga ctctgatatg acctgggttg taatttctga
     3121 tccaatccct gccggcgcat ccattctcac tggagggctc agaaagacaa gtgcagtgga
     3181 aggcgagaaa acggattaca atgtgtaccc tgcttacgag gagagaagtt atgaagcata
     3241 caggatatac tatgaatatg tccctgaagg agagtttaca gtggaatata cagtaagact
     3301 gaaccaggaa ggaaaattca ctgttcctca gacccgcatc gaggcaatgt attctcctga
     3361 tatgaacggc gagactccca acgggcagat ggatgtgtca agagaataat ctatgatgct
     3421 cttcattggg atactctgta tcctgcagct ccttcttacc aactgggggc tgtgggttct
     3481 tctgaaaatc ttccgcgtaa aaagggttca gacaagcgga ggattcttca tattctgtat
     3541 ccctctcctc attttttacg gaagaaatat tcatatcgtt gtcactgaaa aaaaagattc
     3601 agactcagca aatctgaaaa taggaagtat tttttttgca ttaaatcctt tgaagatttt
     3661 tatcggaaag atttatctgg aacattttca tctgtacaga actcatcttt tttatctgaa
     3721 caggattccg tccaaggaga agatcaaata tcttcctccg gtaggaaggg tcagaatcta
     3781 tatcaggaga ctggaaaact gcagcgtgga tgtggaagac agaacagttt ttcctgttta
     3841 cagaaacaca gtcagagaga ttgaactgaa gaatggaaga atggatgctg cagttcctct
     3901 gacagttatt tttttcagcg actacggaag atgcaggata ggctcaggag acattgtcac
     3961 agagatgaga accgatctgc acggaatgct taagattcag ggcattacct ggggagagct
     4021 gatcaatctg gaaggcattc
//
LOCUS       DAOQGJ010000095         4027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12242_length_4027_cov_2.953172, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000095 DAOQGJ010000000
VERSION     DAOQGJ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12242_length_4027_cov_2.953172"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PK683_02375"
     CDS             <1..1111
                     /locus_tag="PK683_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07321.1"
                     /translation="VFTNSVSDKEVNAGRAYVRPYGVIYGIYSLFSGRSSMAYLMSGN
                     VIDEAGRKKVHEELDEEKEEKYIVVERDPNTGEMKEKEKTVPRKEDKDPPPPARETAY
                     DSIFNRFAVGVTSAFDGHAPSQLDFDTTGNLRYDKYNQPKVKYSKRMGIEGYDFEFKP
                     IAKDWIQLTTYVDFNKIKHLDNARGRHNGIILKLGTDNYNITLRPEFRKMSSNYIPMY
                     FDSFYEIERTQANLGSSFPETKYETVNRMTPNGKEVTGYFHSVIINLKKIGLEASYED
                     YSGSNNSRVFAGVYIPIGSFFRLSGFYSKKGFDKSREAFKPDDKAMGAGELALNVGPV
                     TLKIQDRRTWIWDSTANKYQSRDEHLFLFSGGKSF"
     gene            1281..3851
                     /locus_tag="PK683_02380"
     CDS             1281..3851
                     /locus_tag="PK683_02380"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNH07322.1"
                     /translation="MKNTVSLKNLKLEAKESFHEMYGHSGSKIRKEYDHILSHLKSEN
                     PESLRSLRKRIDFVLKEQGITFGESRKNEVIQREWYLDLIPHVISEDEFVFMESALKQ
                     RLSAYNSFLNDIYSERNILKEGIIPKEIILSDPNYIRDCTGIKLPKNIYIHMAAFDVV
                     RSDDGKFLVLDDNMTIPSGISYAMTNRQVLRQQFPRIFQDAPVRQIWDTASAMYSILK
                     ECTPVYDNPLVVLLSPGIFNEAYSEHELLASNMGIPLVLPKDLIVKDNHVYMKTVHGL
                     ARVDVIYRRIQDYYLDPVCFYQDSVLGVPGLFSCVRNGNVTVANAIGSGIASSKALLP
                     FVDEIIRFYTKKEPLMHTVPSFLLSNPKDREYIFNHIHQFVIKPIHGTGGSGILIGCE
                     STVSQVEAFRKKTDADPLGYIAQKLIPLSKSRIFDQKGFAERFVETRFYGFQGSSFYL
                     SSCALTRVSPEDNTMMVCNSMGGGSKDTWVLGKSISYSKNEKNFSYASGKRLLLSRVA
                     ENLFWLGRYVNRGFTTANVLLIAYTSEIDILLGQGDPSFNSLIKTLSKITRTPVKSFL
                     KKGRGWQMEFFRGAITDAKNPSSMISSINYAMNNAREIQSYLSNDMWVSLKKLTEYLS
                     MLPVREGGSINVEDLSQWLVGVIHYAQSFYGSALDTFSRQDILQFIQLGRYVEHCSNI
                     ITVLKNTIQFLVRIIGEKTQFPNIQPFIIVLLKILNSYEAYLWYHQSIFDPYLAYNMI
                     TTDRDFNNSLVSTLDKMKQILTSMRNEENVPEDETSPEYICDILISRAFSFNLRENLG
                     KTSPEYKVRKFEHFLYTKNEVHPGYWANHLKAGIELLGGKIMDRYSNITSPTSFTAGI
                     "
     gene            3848..>4027
                     /locus_tag="PK683_02385"
     CDS             3848..>4027
                     /locus_tag="PK683_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HNH07323.1"
                     /translation="MMQRYSLIHRTLYEYDYEATESYTRVKITPIETFCQSVHSHTIE
                     VLPDVTLYKHDDYFGN"
BASE COUNT         1267 a          847 c          870 g         1043 t
ORIGIN      
        1 ggtatttacc aattcggtga gcgacaagga agtcaatgca gggcgtgctt atgtgcgtcc
       61 atacggagtc atttacggta tctacagtct tttttcaggc aggtccagta tggcctatct
      121 gatgtcagga aatgtcatag acgaagccgg aagaaaaaaa gtgcacgagg aactggacga
      181 ggaaaaggaa gaaaagtaca ttgttgtaga aagggacccg aatacgggag aaatgaagga
      241 aaaggaaaag acagttccca ggaaagaaga caaggaccct ccccctccgg caagagagac
      301 ggcatatgat tccattttca accggtttgc agtcggtgtg acttcagcgt ttgacggaca
      361 tgccccaagc cagcttgatt tcgacacgac aggaaatctt cggtacgaca agtacaatca
      421 gcccaaagtc aaatactcaa aaagaatggg aattgaagga tacgactttg aattcaagcc
      481 aatagcgaag gactggattc agctgaccac ctatgtggat ttcaataaga ttaagcatct
      541 ggacaatgcc agaggcagac ataacggaat catattgaaa ctgggaaccg acaactacaa
      601 tatcactctc cgccctgaat tcaggaaaat gagttccaat tatattccca tgtattttga
      661 cagcttttat gaaatagaaa ggacacaggc caatctggga agcagttttc ctgaaacaaa
      721 gtacgaaaca gtgaacagaa tgactccaaa tggaaaagaa gtcacagggt attttcactc
      781 tgtcatcatc aatctgaaaa aaatcggctt ggaggcaagt tatgaggact attcaggaag
      841 caataattcc agagtctttg cgggagtcta tatcccgatt ggatcatttt tcagactttc
      901 cggtttttat tccaaaaaag gttttgataa aagcagggaa gcatttaaac cggatgacaa
      961 agcaatggga gcaggagaac ttgccctgaa cgtaggtcct gtgactctga aaattcagga
     1021 cagaagaacc tggatctggg attctacagc aaataagtac cagtcaagag acgagcacct
     1081 gtttctgttt tcagggggaa agagtttctg aaactgaaag tgttatgtat ttttttatat
     1141 ggacgtttaa cggatttttc aatggcgatg ctctcaagca tcccggcctg cgaatctccc
     1201 ggtagtgtcc aaaatatcag cggatgcaat gctgcaatgc cgaacatctg ctcaaaaact
     1261 gcaaaggaac cgatatacat ttgaaaaata ctgtctcact gaaaaatctg aagctggaag
     1321 caaaagaaag ctttcacgaa atgtacggtc attcgggatc gaaaatcaga aaggaatacg
     1381 atcatatcct ttctcatctg aaaagtgaaa atcccgaatc attacgatcg ctcagaaaaa
     1441 gaatagactt cgtactgaaa gaacagggga tcactttcgg agaaagcaga aaaaatgaag
     1501 tgattcagag agaatggtat ctggatctga ttcctcacgt catcagcgaa gatgagtttg
     1561 tctttatgga aagtgccttg aaacagaggc tttctgcata caactccttt ctgaacgata
     1621 tttattcgga aagaaacatt ctgaaagaag ggatcattcc taaggaaatt atcttatcag
     1681 atccgaatta tatccgtgac tgcaccggaa taaaacttcc gaaaaacatc tatatccata
     1741 tggccgcatt tgacgttgta cgctctgatg acggaaaatt tcttgtgctc gacgacaata
     1801 tgacgatccc aagcggtatc agttatgcaa tgaccaacag acaggtgctc cgtcagcagt
     1861 tccccagaat ttttcaggat gctcctgtcc gtcagatctg ggatacagca tctgcaatgt
     1921 acagcatact gaaggaatgc acccctgtgt acgacaatcc tcttgttgtt ctgctgtctc
     1981 ctggaatatt caatgaagca tattctgaac atgaacttct cgcatccaat atggggattc
     2041 ctcttgttct tcccaaggat ctgattgtaa aagacaacca tgtgtacatg aagactgtgc
     2101 acggacttgc cagagtggat gtcatttaca gaaggattca ggactattat cttgatcctg
     2161 tctgcttcta tcaggacagt gtactcggtg ttccgggact tttttcctgc gtaagaaacg
     2221 ggaatgtgac tgtggcaaat gccatcggaa gcggaatcgc cagttcgaaa gcacttcttc
     2281 ctttcgtaga cgagattatc cgtttttata caaaaaaaga acctctgatg catactgtcc
     2341 cttcctttct tctctccaat ccgaaggaca gagaatatat tttcaatcat attcatcagt
     2401 ttgtaattaa gcctattcat ggaaccggag gaagcggaat actgatcgga tgcgaatcta
     2461 ctgtctctca ggtggaagct ttccggaaaa aaactgatgc agatcctctc ggatacattg
     2521 ctcagaaact cattcctctg tccaaaagca gaatattcga ccagaaaggt tttgcagaac
     2581 ggtttgtgga aaccagattt tacggattcc agggaagttc cttttatctc agcagctgtg
     2641 cattgaccag ggtttctccg gaagacaata caatgatggt ctgcaattcc atgggaggcg
     2701 ggagcaagga cacctgggtc ctcggaaaat ccatatccta ttccaaaaat gaaaagaatt
     2761 tttcctatgc ctcaggaaag agacttcttc tttccagggt ggcagaaaat cttttctggc
     2821 tcggaagata tgtgaacaga ggattcacga ctgcaaacgt tcttctgatt gcatacactt
     2881 cagaaataga cattcttctg ggtcagggcg atccttcttt caacagtctc atcaagactc
     2941 tttccaagat cacaagaaca ccggtgaaaa gttttctgaa aaaaggaaga ggctggcaga
     3001 tggaattttt ccgcggagcg atcaccgatg cgaagaaccc atcctccatg atatcgagca
     3061 tcaactatgc aatgaacaat gccagagaga ttcagagtta tctttccaat gacatgtggg
     3121 tatcattaaa aaaacttacg gaatatctct ccatgcttcc tgtcagagaa ggaggttcga
     3181 tcaatgtgga agatctgtct cagtggcttg tgggagtaat ccattatgca cagtcattct
     3241 acggatcggc actcgataca ttttcgagac aggacatact tcagttcatt cagcttgggc
     3301 gttatgtaga acactgctca aacatcatca ctgttctgaa aaacacaatt cagtttctgg
     3361 tcaggatcat cggcgaaaag actcagttcc cgaacattca gcctttcatc attgtgcttc
     3421 tgaaaatact gaattcctac gaagcatatt tatggtatca tcagtccatt tttgatcctt
     3481 atctggcata caatatgatt actacagaca gagacttcaa caattcactt gtttcaactc
     3541 tggacaagat gaaacagatt ctgacttcca tgagaaacga ggaaaatgtg cctgaggatg
     3601 aaacatcgcc cgagtacatc tgcgatattc tcatcagcag ggcattttca ttcaacctga
     3661 gagaaaatct gggaaagact tccccggaat acaaagtgag aaaatttgaa cattttttat
     3721 atacaaaaaa cgaggtccat cccggatact gggcaaatca tttgaaggca ggcattgaac
     3781 ttctaggcgg gaaaatcatg gacagataca gcaatatcac atctccgacc tcatttacgg
     3841 caggaatatg atgcagagat acagccttat tcacagaacc ttatacgaat acgattatga
     3901 agcaaccgag tcctacacca gggtgaaaat cactccgatt gaaaccttct gccagtcagt
     3961 tcattctcat acaattgaag ttctgcccga tgtcacactg tacaaacatg acgactattt
     4021 cggaaat
//
LOCUS       DAOQGJ010000096         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12315_length_4014_cov_2.783784, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000096 DAOQGJ010000000
VERSION     DAOQGJ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12315_length_4014_cov_2.783784"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK683_02390"
     CDS             complement(<1..424)
                     /locus_tag="PK683_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH07324.1"
                     /translation="MSNIKYSVFIAVSADGYIAGKDGALEWLSAVSREGEDYGYLEFF
                     STVDTLVMGRNTYEKVLSFGDWPYEKKDCIVLTKEKLKPKFNEKIMNLSPIELDHFLQ
                     KEEKRKVYLDGGKLISSFLEAGFVDEIILSVIPVLLGEG"
     gene            complement(438..929)
                     /locus_tag="PK683_02395"
     CDS             complement(438..929)
                     /locus_tag="PK683_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH07325.1"
                     /translation="MSGFQAKDTELLRNSAVLKGLSEMEIKQLLDSSELKTGARNDIL
                     LNEWEIGRGIFIILEGEVEVYLPKQNKRGIERISEVKVSRLKSGDCFGEYSLIDEGRI
                     SASVRVVSQNFRASYINQKDFQSFSEIHHKSAKIIYRNLLEIFIARLRGNLEELDMVY
                     VIH"
     gene            1127..1687
                     /locus_tag="PK683_02400"
     CDS             1127..1687
                     /locus_tag="PK683_02400"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH07326.1"
                     /translation="MLDILEIPEIRRQFVPLSLQEYEDLCIREPEKYRKTELFRGAVV
                     TKMTKSSRHSYFKNVFTEDIRKVVPSGFFVQNGNSISFLDSDLESDISVIAGNQKDYR
                     NSKPSMARLIVEIAVSSIVYDRSKSMDYSRAGVNEYWIVDADSESVEVYTHPSENGYQ
                     QKKLFVKEDSLELFGKLFPLNRIIEQ"
     gene            1809..2357
                     /locus_tag="PK683_02405"
     CDS             1809..2357
                     /locus_tag="PK683_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07327.1"
                     /translation="MKKTAEKFKSKFRPYEVPAKLLALADYWDKNPEFFSGSFEVDSD
                     EYDSAVDWFRKNEEGYSKVRVFGIDGIHSLYAFWTAEESDLEKCPIVYLGGEGEGTVV
                     LAENFDILLSILASNREYEPFDKDFMEEETSNEEENKRFRKWLQKNYNILPAKNPLSL
                     MKKAQKRFPGFQKWLKTVIPGR"
     gene            complement(2364..3068)
                     /locus_tag="PK683_02410"
     CDS             complement(2364..3068)
                     /locus_tag="PK683_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07328.1"
                     /translation="MFKFFYLLLSVILFTDCVSGFKTKKLLNATAKAAFYDLVVDELE
                     SEVGRTDLINGTITRPFEIDPDKLKNILGNIRFVKHTRVNKYEDYIFHDDELDTLAEN
                     LARALMNPPKTKKESIVVVISQFDHLQSVISNNKRTSFYAWAGQDGLNLVFGDIQYDV
                     ARDKARNFYDWTSVDPIPLTNRRDENEIRSNKDIFKFKEIDGFENNKWLLFALDDLNK
                     YSPRPKLGTKDKEGAN"
     gene            complement(3071..3433)
                     /locus_tag="PK683_02415"
     CDS             complement(3071..3433)
                     /locus_tag="PK683_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH07329.1"
                     /translation="MVSLIHFANNHLLVSIEGNILMENSKDFQNSVQALLSGYPALGM
                     LTFDFRKVQFMDSSGMGILIRTVADLKKKNCTVNAVSLNKNLYAVFHLSGLHTIIKIH
                     IQEDFYKAYPDFEKYKGI"
     gene            complement(3433..3621)
                     /locus_tag="PK683_02420"
     CDS             complement(3433..3621)
                     /locus_tag="PK683_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07330.1"
                     /translation="MKAGDKAKLLKKTFIQNGIFIISGSVVEIAEILDEEIRVMYSDK
                     EGYPHVVTGLKEADLAVI"
     gene            complement(3618..>4014)
                     /locus_tag="PK683_02425"
     CDS             complement(3618..>4014)
                     /locus_tag="PK683_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNH07331.1"
                     /translation="TRAKAMQILSEKGSLSNEDLSSIILEKEKLDGISFSGANLHNAF
                     LQNSSFRNSDFSGANLKGAFLNNCDLSGANFRGADLRFAKLTGAVVEGTDFTDALYDI
                     GTRVDSRNLEIFTVMKKTGKEIYVAKEAV"
BASE COUNT         1144 a          847 c          771 g         1252 t
ORIGIN      
        1 ttccttctcc aagcagaaca gggatgacag aaagaataat ctcatccaca aaacctgctt
       61 caaggaagct gctgatgagt tttccgccgt ccagatacac tttccttttt tcctctttct
      121 gaaggaaatg atcgagttca ataggagata aattcatgat tttttcatta aatttcggct
      181 tcagtttttc tttagtaagg acaatacagt cttttttttc gtaaggccag tctccgaaac
      241 tcagcacttt ttcataagtg tttcttccca tcaccagagt gtccactgtg ctgaaaaatt
      301 ccaggtatcc gtagtcctct ccttccctgg aaactgccga aagccattcg agagctccgt
      361 cctttcctgc aatgtaaccg tccgcactca ccgctataaa tacagaatat tttatatttg
      421 acatataatc tcctttttta atgaattaca taaaccatgt ccagttcttc cagattcccc
      481 ctgagtcttg caataaaaat ttcaagaaga ttcctgtata taatctttgc tgatttatga
      541 tgaatttctg aaaatgactg aaaatctttc tgattgatgt agcttgctct gaagttttgg
      601 gagacgactc tgacggatgc tgaaattctg ccctcatcga ttaaggaata ttcgccgaaa
      661 cagtctcccg atttcagtct ggaaaccttc acttccgaaa ttctttcgat tcctcttttg
      721 ttctgcttgg gcagatatac ctccacctca ccttccagaa tgatgaaaat gcctcttcct
      781 atctcccatt cattcagaag gatgtcattt cgggctccgg tcttaagttc acttgaatcg
      841 aggagctgtt ttatttccat ttcgctcaat cccttcaata ctgccgagtt tctcagtaat
      901 tccgtatcct ttgcctgaaa accgctcata gtattctcca ttctgttctg attaaattat
      961 tcactgcact ctgcagatgc cgtctgcctg cataccgaaa accttgattt cacagccgct
     1021 ctgcctgata aaatacagaa aaagccgagc aaaaaaacag gctattcatt tttaacagtt
     1081 cgacattatg attgacagaa tctgttttat cagatattga atgagtatgc tggatatttt
     1141 agaaattcct gaaatcagaa gacagtttgt tcctctcagt ctccaagagt atgaggatct
     1201 ctgtattcgg gagccggaaa aatacagaaa gactgaactt ttcagagggg cagtcgtaac
     1261 gaaaatgaca aaaagttcca ggcacagtta ttttaagaat gtatttacgg aggatatcag
     1321 aaaagtggtt ccttcaggtt tttttgtgca gaatggaaat tctatctctt ttctggattc
     1381 ggacttggaa tctgacattt ctgtgattgc aggaaatcag aaagattaca gaaacagcaa
     1441 gccgtctatg gccagactga tagtagagat agcggtcagt tccattgtat atgaccggag
     1501 caaaagtatg gattactccc gggcaggagt caatgagtat tggatcgtag atgctgattc
     1561 tgaatccgtt gaagtataca ctcatccttc agaaaacggg tatcagcaga agaaactttt
     1621 tgtaaaggaa gattctctcg aactctttgg aaaactgttt ccattgaaca ggattattga
     1681 acagtaaaaa aaattattta catcccgatg caaaaattct taatgaaaaa ttaactttac
     1741 acttccggac aatattttcc tcttttgtaa atcagcagga agactcttcc ggaaataagg
     1801 aggaatttat gaaaaagaca gctgaaaaat tcaagagtaa atttcggcct tacgaggtgc
     1861 ccgcgaagct tctggctctg gccgactatt gggacaaaaa tcctgaattt ttttccggat
     1921 cctttgaagt ggattcagac gaatacgatt ctgcagtgga ctggtttcgg aaaaatgaag
     1981 aaggttattc gaaagtgaga gtgttcggaa tagacgggat acattcactg tatgcgttct
     2041 ggacggcgga agaatcggat cttgaaaaat gtcctatagt ttatctggga ggggaaggag
     2101 agggcactgt tgttcttgcg gaaaattttg atattctgct ctccattctg gcttccaaca
     2161 gagagtacga accgtttgac aaggacttta tggaagaaga aacttcaaac gaagaagaga
     2221 ataaaagatt caggaaatgg cttcaaaaaa attataatat attgcctgca aagaatcctc
     2281 tgagccttat gaaaaaggcc cagaaaagat ttcctggatt tcaaaaatgg ctgaaaactg
     2341 tcatccccgg caggtgataa ttttcagtta gcaccttctt tatccttggt tcccagtttc
     2401 ggtcttggac tgtatttgtt cagatcatcc aatgcaaaca gaagccattt attgttttca
     2461 aatccgtcaa tttccttgaa tttgaaaata tccttattcg atctgatttc attttcatcc
     2521 cgtctgtttg tgagaggaat cggatctacg ctcgtccaat cgtaaaaatt cctggccttg
     2581 tctcttgcaa cgtcatactg aatgtctccg aataccagat tcagcccatc ctgcccggcc
     2641 caggcataaa aggatgttct cttattgttg gaaatgacag actgaagatg atcgaactga
     2701 ctgatgacca ctactatgct ttccttcttt gtctttggag gattcataag cgcccttgcc
     2761 aggttttctg caagagtatc cagttcatcg tcatgaaata tataatcttc gtacttattg
     2821 actctggtat gttttacaaa tcgtatgttt cccagtatgt tcttcagttt gtccgggtca
     2881 atttcaaacg gtcttgttat agtaccgttg atgagatcag ttcttcctac ttcagattcc
     2941 agttcatcca ctacaagatc gtaaaatgca gccttggccg ttgcattcag cagctttttg
     3001 gtcttgaaac cggatacaca gtctgtaaac agaataacag agagaagcag atagaaaaat
     3061 ttgaacattt ttaaattcct ttgtattttt caaaatcagg atatgccttg taaaagtctt
     3121 cctgtatatg aattttaatg atggtatgga gtcccgaaag atggaacact gcataaaggt
     3181 ttttattcag agagacggca ttgacggtac agtttttctt tttcaggtcg gcaacagttc
     3241 tgatcagaat tcccattccg gaggagtcca taaactgcac ctttctgaaa tcaaaagtca
     3301 gcattcccaa agccggataa ccggaaagaa gtgcctgcac tgaattttgg aaatccttgg
     3361 aattttccat cagaatgttt ccttcaatgc tcaccagcag atggttgttt gcaaaatgaa
     3421 tcagacttac catcagatta ccgccagatc agcttctttg agaccagtaa ctacgtgagg
     3481 ataaccctcc ttgtcgctgt acatgactct gatttcttcg tcgagaattt cggcaatttc
     3541 gacgaccgaa cctgaaatga taaatatccc gttctgaata aaggtttttt ttaaaagctt
     3601 tgctttgtcc cctgctttca cactgcctct tttgcaacgt agatttcttt gcctgttttt
     3661 ttcatcactg taaaaatttc cagatttctg ctgtccactc gagtgcctat atcgtacaga
     3721 gcatcggtaa aatcagttcc ttccaccact gcacctgtca gttttgcaaa acgcagatca
     3781 gcccctctga aatttgcacc cgacagatcg cagttattca gaaaagcgcc ttttaaattt
     3841 gcacctgaga aatctgaatt tcgaaaactg ctgttctgca ggaaagcatt gtgcagattt
     3901 gctcctgaaa aactgatccc gtccagtttt tccttttcca aaatgatgct cgaaagatcc
     3961 tcattggaga gagagccttt ttctgacaga atctgcatcg ccttcgctct tgta
//
LOCUS       DAOQGJ010000097         4006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12362_length_4006_cov_3.015945, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000097 DAOQGJ010000000
VERSION     DAOQGJ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12362_length_4006_cov_3.015945"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1378
                     /locus_tag="PK683_02430"
     CDS             50..1378
                     /locus_tag="PK683_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07332.1"
                     /translation="MGSPGWEEIYISSQEEESFSEGFATGSFSITEIRDYFVDKSGNP
                     SSESSWQFLSFFPGEKNPTFNLTDFLYTDLLGNIRLRLGKNAKYSFLEPILQNPSFKE
                     SLERILTDLMSRPELHTDYLDTVFLTLLYQNRLKEISEFIMSLSSKNLLSLNLQIFSI
                     LSGLMKKPLENLVMSGAEKEPLNILYKYKFLEMSLLERNRLYDLVLEKTAPSLNGIVF
                     HLFSRNTSGIRNIFPIYKIIMENFDSLDDLEKKEFMESFQENGRFLQVYNFMKKSYSK
                     RELRAWLDRIRRLNGKSDPDTEHSFKGYSEKEDLIQKYKTLRAEHMVYTLSPFEILLL
                     LSSSVALQVKNEIMEAYKSIPSSYIVNRSLSAVYYFQSDYRKFLLHLERSGSLQNHAE
                     SIYLKSLACIELGHNEEAKRLLLVLKSKFPDADVIQDGLKKLARKTAAAV"
     gene            complement(1414..3213)
                     /locus_tag="PK683_02435"
     CDS             complement(1414..3213)
                     /locus_tag="PK683_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07333.1"
                     /translation="MKILYFFLFSFSVLFSDSGDFRLDSEKRYRTPEECISADKRKEV
                     EDISSKDSGSSEKIQKILETLELYYGQFTEDKRLEEERLRAGKFPDRKFRDEVSLRFK
                     ILSGKTGPTTDWNSIRNFGRMQTLQNSEASLIRNSRILYDLFFQLGQLYEKNSEDHKA
                     KEAYLAAFRNHSFDFGEDYFLKEESNQDIFSNENSGRISRHRELLSEFQKKKKEVREL
                     IDSVHEQEAVNARKNVSSDFAGNRKKIEDAEKGLENAEKKYRDSFQENLSPVQKEKTS
                     IDAKRLFIFGNIMKKNAKHFGYISIYELAYSLNPEMPELLMELGDEYRKAKDTRTALD
                     YYIKYIRLEKDRFKESKFDAGVYLKAGTLHSELKENIPAVKYFEEYYSSLVGSDDKQK
                     TEFSFFLSDFYDKRLGNLASAALYADRWISENEKSKSDKSVRSINEIRKSFFCNWIIS
                     RNEKQKKRFQTEDSYLEKTYNDYKSLKDSLQKSDETVNAKRTEVNGLKRQLIYSTDPK
                     VLEVYKEEEKSLNLLTESNQEIRSAFKNLYSIPLLLRIAEREEEKRNYEKAYSMFQEI
                     QGLGNETESEKAVRSMIRIQNIISDGRIREKDD"
     gene            complement(3254..3718)
                     /locus_tag="PK683_02440"
     CDS             complement(3254..3718)
                     /locus_tag="PK683_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH07334.1"
                     /translation="MPQEENHLIDQYITFTIGEEDYAISIINVEEIVKVSNLIKIPKS
                     QDYFVGLMDIRGKVVNMIDLSKKIMNKRVHESMMNRAIIVKINGKSLGIIVDKVSHVV
                     QFSKSQIDPPPPSVKGMSSRYIIGVAKRDNRFIILIDIEKILTNEELAEVTG"
     gene            complement(3734..>4006)
                     /locus_tag="PK683_02445"
     CDS             complement(3734..>4006)
                     /locus_tag="PK683_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07335.1"
                     /translation="RERLRGFLRPKFSRERVIDELHDAVLGASLGAILYLHYFDYIVV
                     GSGLAFLGIMFGGMDWVIRGRNPLIMKVLIFLSLGSYFFYTGYTEF"
BASE COUNT         1138 a          853 c          729 g         1286 t
ORIGIN      
        1 cggttaggct cgtatgccta ttgtaaatta atatatagga gagaaaaaat tgggttctcc
       61 aggctgggaa gaaatataca tttcctcaca agaagaggaa agcttttctg aaggatttgc
      121 tacaggcagt ttttcgatca cagaaatacg cgattatttt gtagacaaat cgggaaatcc
      181 gtcttcagaa tcgtcctggc agtttctgtc cttttttccg ggtgaaaaaa acccgacttt
      241 taatcttacg gacttccttt acacagatct tttaggaaat atccgtctgc gtctcggcaa
      301 aaatgccaaa tactcctttc tcgaacccat tcttcaaaac ccgtccttca aggaatctct
      361 cgaaagaatc ctgacggatc tgatgagcag accagagctt catactgact atctggatac
      421 agtttttctg acacttttgt accagaacag gctgaaagag atctctgaat tcattatgag
      481 cctttcttcc aaaaatcttc tcagtctgaa tcttcagata ttctccattc tttccggact
      541 gatgaaaaag cctctggaaa atctggtaat gagcggtgca gaaaaagaac cgttgaatat
      601 cctttacaaa tacaaattcc tggaaatgtc gcttctggaa agaaatagac tgtacgatct
      661 ggtcttggaa aaaacggctc cttcgttgaa tggaatcgta tttcatcttt tcagcagaaa
      721 tacttccgga atcaggaata ttttccccat ctacaagatt ataatggaaa actttgattc
      781 attggatgat ctggaaaaga aagaatttat ggaatccttc caggaaaacg gacgtttcct
      841 tcaggtgtac aattttatga aaaaatcgta cagtaaaagg gaactcaggg cctggcttga
      901 cagaatccgc agactgaacg gaaaatctga cccggacaca gaacactctt tcaaaggata
      961 cagtgagaaa gaagatctga ttcagaaata caaaactctc cgggccgaac atatggtgta
     1021 tacactctcc cctttcgaaa tacttcttct gctcagttca tcggttgctc ttcaggttaa
     1081 aaatgagatt atggaagcat acaaatccat tccatcctca tatattgtga acaggtccct
     1141 ttctgcagtg tattactttc agtctgacta cagaaaattt cttcttcatc tggaaagatc
     1201 gggaagcctg cagaaccatg cagaaagcat ttatctgaaa agtcttgcct gcattgaatt
     1261 aggacacaat gaagaggcta aaagacttct tctggtttta aaatctaaat tcccggatgc
     1321 tgatgtcatt caggatggac tcaaaaaact ggctcgaaaa actgcagcag cagtgtaact
     1381 gcggcagttt aactgcggca gtagaagaac agctcagtcg tctttttctc tgattcttcc
     1441 gtctgaaatt atattctgaa ttctgatcat acttctgaca gccttttcag actctgtttc
     1501 atttccaagt ccctggattt cctgaaacat actgtacgct ttttcataat ttcttttttc
     1561 ttcttctctt tctgcaattc gaaggagaag cggaatggaa taaagatttt taaatgccga
     1621 tctgatttcc tgattggatt ccgtaagaag attcagtgat ttctcttctt ccttgtacac
     1681 ttccagaact ttgggatctg tggaataaat cagctgccgt ttgagaccgt tgacttccgt
     1741 cctttttgcg tttactgtct catcactttt ctgaagtgaa tctttcaagc ttttataatc
     1801 attgtacgtt ttttcgagat acgaatcctc agtctgaaag cgttttttct gcttttcatt
     1861 tctggaaata atccagttgc agaaaaatga ttttctgatt tcattaatgc ttctgacaga
     1921 cttatccgat tttgactttt cattctctga aatccatctg tctgcataca gtgccgcaga
     1981 ggcaagattt cccagtctct tatcataaaa atcggaaaga aaaaaactga actcagtctt
     2041 ctgtttatca tccgatccaa caagactgga atagtattcc tcaaagtatt tcactgcagg
     2101 aatgttttcc ttcagttctg aatgaagagt gcctgctttc agatagacgc ctgcatcaaa
     2161 tttggattcc ttgaaccggt ctttctcaag tctgatatat tttatataat aatcgagggc
     2221 tgtccttgtg tctttcgctt ttctgtattc atctccgagc tccataagaa gttcaggcat
     2281 ctctggattc agagaatacg caagttcata gatggaaata tatccgaaat gttttgcatt
     2341 ttttttcatt atatttccaa aaataaaaag tctcttcgca tctattgagg tcttttcttt
     2401 ctggacggga gaaagatttt cctgaaagga atctctgtat tttttttctg cgttttcgag
     2461 acctttttcg gcatcttcta tcttcttcct gttccctgca aaatcagaag aaacattctt
     2521 tcgggcattg accgcctcct gctcatgtac agaatcaatg agttcacgga cctctttctt
     2581 ctttttctga aattctgaaa gaagctcccg gtgcctggaa atgcggccgg aattctcatt
     2641 ggaaaaaata tcctgattgc tctcctcttt cagaaaatag tcttctccga aatcaaaact
     2701 gtgatttctg aatgcggcaa gatatgcttc cttcgccttg tgatcctcac tgtttttttc
     2761 atacagctgg ccaagctgaa aaaagaggtc atacagtatt ctggaatttc tgattaatga
     2821 tgcttccgaa ttctgaagag tctgcatcct cccaaaattt cggatcgaat tccagtctgt
     2881 ggtcggtccc gtttttccag aaagtatttt gaatcttaat gaaacctcgt ctctgaattt
     2941 cctgtcagga aatttccctg cacgaagcct ttcctcttca agtcttttgt cttcagtgaa
     3001 ctgtccgtaa taaagttcga gagtttccag aattttttga attttttcag aactgccgga
     3061 atctttagat gaaatgtctt caacttcttt tcttttatct gcactgatac actcttccgg
     3121 ggttctgtat cttttttctg aatccagacg aaaatcgccg gaatcggaaa ataaaacaga
     3181 aaaactgaaa agaaaaaagt ataatatctt catcagtttc tgtatcggca gaaatgcatt
     3241 cagtgaaaag cgttcagccg gtaacttcag ccagttcttc gttcgtgaga attttttcga
     3301 tatcaatcag aatgatgaat cggttgtctc ttttggccac tccgatgatg taccgggaag
     3361 acattccctt taccgacgga ggcggaggat ctatctgcga tttggaaaac tgcacaacat
     3421 gagaaacctt gtctacaatg attcccaagg attttccgtt gattttcaca atgatcgcac
     3481 ggttcatcat tgattcgtgc actcttttat tcatgatctt tttgctcaga tcaatcatat
     3541 tgactacttt gccgcgaatg tccatgaggc ccacaaaata gtcctgtgat ttaggaattt
     3601 taatcagatt ggaaactttt acaatctctt ccacattgat aatggaaatt gcatagtctt
     3661 cttcgcctat ggtaaatgtt atatactgat caatgagatg attttcttct tgcggcatag
     3721 tcagactccc attttagaac tcagtatatc ctgtatagaa aaaatagctt cctaaactta
     3781 aaaaaatcaa gaccttcata atcagaggat ttcttccccg aatcacccag tccattcccc
     3841 caaacataat acccagaaag gcaagtcctg aacctacaac aatgtagtca aaatagtgaa
     3901 gatagagaat tgcacccaga gaagcaccta gaaccgcatc gtgcagttcg tcgattactc
     3961 tttccctgct gaacttcgga cgaaggaatc cgcgaagcct ttctct
//
LOCUS       DAOQGJ010000098         4004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12372_length_4004_cov_2.962775, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000098 DAOQGJ010000000
VERSION     DAOQGJ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12372_length_4004_cov_2.962775"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PK683_02450"
     CDS             complement(<1..746)
                     /locus_tag="PK683_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNH07336.1"
                     /translation="MNSQNKDSNFKTILFLLLFLSAWDIFAIAVRGRVYSRQKSKGEG
                     GVQVFVTETKKAYTTDADGFFDAELPSAGDFTFKIVRPTGEITSIGKNVSQDGELINL
                     YTDKVEVPKGAINVEGTKDKTILSRYKLKYDEIKRLPGTMGEALNALQTVPGVFQPPF
                     FGGGNGAQPGAFVCRGANPNSNTVLYDDLPIAYAYHFDSLNSVIHNDLIKTIDIYTGA
                     YPANFANATGCVIEIESSDKPPKRSGQANLS"
     gene            complement(715..1044)
                     /locus_tag="PK683_02455"
     CDS             complement(715..1044)
                     /locus_tag="PK683_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07337.1"
                     /translation="MKILSVLLLFLGCIPYEEKIPKWNAQTQLNAAVDFKAKECKAPA
                     PQPPLYVFTDQHKRNLDLCTIAITKIACPFNDYPIICLGIYYTKETPNLPWYVNFNEL
                     TKQRFKL"
     gene            complement(1044..1460)
                     /locus_tag="PK683_02460"
     CDS             complement(1044..1460)
                     /locus_tag="PK683_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07338.1"
                     /translation="MRLVILFLSAVLLNISCAGGLTEKGGRVQNIQIAPSATLIRSSK
                     YRQLDYTEGESSQFFLLGLIPVTEHLSIDYAMSQAVQKVQGGDSLADMQIWHEVHYFF
                     PLGTVSVVKVGGYAVGLKDTQPLFDKGPDGKKAGKK"
     gene            complement(1462..1800)
                     /locus_tag="PK683_02465"
     CDS             complement(1462..1800)
                     /locus_tag="PK683_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07339.1"
                     /translation="MKKLVLLVLLIIYSAYGCATANAGFATSNIPIAERKYKVIGSVE
                     KEKGWVTLDFAIIGIPLKKPPVSSLLDEALKEKNADALVNIRYWNDRMIFFFITWNRM
                     GLTAEAVKFD"
     gene            complement(1797..2303)
                     /locus_tag="PK683_02470"
     CDS             complement(1797..2303)
                     /locus_tag="PK683_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH07340.1"
                     /translation="MDRELQISSELSSFINGLGQWYESMGISRIAGQICGLLLVADEP
                     VTPEDISRILNVSRSSVSTNLKMLKMVGLLEYRRISGDRKEYILFSDFALENSLRIKI
                     ESYSPFEKMVDESLGFLKKGSKAEKRMKDLKEYLKLEKGFTVSLMEKWKRLSESRTSA
                     KHDKGRNK"
     gene            complement(2416..3831)
                     /locus_tag="PK683_02475"
     CDS             complement(2416..3831)
                     /locus_tag="PK683_02475"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH07341.1"
                     /translation="MLTKKILADRNLYAILEFCSMLPLLEIISVLKKISLFSETPENS
                     IAEIAQSLKQEFFGQGRTVLQIGDPGTCMYIIYSGSVSVHKEGQKFAEVGPMQTLGEF
                     SLLNSEPRNATVIAKEDCILLRLDAADFYRIMGSSTEILKGIIKILINRLNIQNSELI
                     DTLKRREAELTRLVDERTKDLQNAMHEISAKNEALEKSFNEISVQKSIIEEKNRNITE
                     SIEYAKTIQKSILPLSEQVKSVLPDSFILYRPKDVISGDFYFFHSQKYQTGDEKDNIA
                     VIAAIDCTGHGVPGAMMSMIGSAILNETVGEKRILIPSEILSELHKAIRYALNQENTK
                     SRDGMDLSLCMIDLRRKILNFSGARNSLYYVENDTVVRIRGTKKSIGGVQKEEERIFE
                     SHTLPIENPLCFYITTDGYLDQFGGENREKFSQERFKNLILEIHALPMNEQKKIFEER
                     ISQWMGDHEQIDDMLLIGVKV"
BASE COUNT         1003 a          913 c          823 g         1265 t
ORIGIN      
        1 gaaagattgg cctgtcccga tcttttcgga ggcttgtcgc tggattcaat ctcgatcacg
       61 cagcctgtag catttgcaaa gttcgcagga taggctcccg tataaatgtc aattgtcttt
      121 ataagatcat tatggatgac cgagttcaga ctgtcaaaat ggtatgcata ggcaatcgga
      181 agatcatcat acaggactgt atttgaattc ggatttgctc cccggcatac aaaagcacca
      241 ggctgtgcac cgtttccgcc tccgaaaaac gggggctgaa atactccagg cacagtctga
      301 agtgcgttca gggcctcacc cattgttcca ggaagtcttt tgatttcatc atacttcagt
      361 ttgtatctgg aaagtattgt cttgtccttt gttccttcca cattgatggc gcccttcggc
      421 acttccacct tgtctgtata cagattgata agttcaccgt cctgagacac attttttccg
      481 atggaggtga tttctccagt gggtcttaca atcttgaacg tgaaatctcc ggctgagggg
      541 agctctgcat cgaaaaaacc gtcagcatct gtagtatatg ctttcttagt ttcagtgaca
      601 aatacctgca cgcctccctc tcccttgctt ttctgtctgc tgtaaacgcg tccccttacg
      661 gcaattgcaa atatgtccca cgcagaaaga aacagcagga gaaacaggat ggttttaaag
      721 tttgaatctt tgttttgtga gttcattgaa gtttacgtac cagggaagat ttggagtttc
      781 ttttgtataa tatatcccga gacagataat gggataatcg ttgaaagggc aggcgatttt
      841 agtgattgca attgtgcaca gatccaggtt tcttttgtgc tgatccgtaa atacatacaa
      901 tggaggctgc ggagcagggg ctttgcattc cttggccttg aagtccacag ccgcattgag
      961 ctgggtctgt gcattccatt ttggaatttt ttcctcataa ggaatgcatc ccaaaaacag
     1021 taaaaggaca gataatattt tcattatttc tttcctgcct tttttccgtc aggccctttg
     1081 tcaaacagag gctgagtatc cttgagaccg actgcatatc cgccgacctt gacgacagac
     1141 actgttccca aagggaagaa ataatgcacc tcatgccaga tctgcatatc agcaagagag
     1201 tctccgcctt gtaccttctg tacagcctga ctcattgcat agtcaatgct taagtgctct
     1261 gtcacaggta tcagtcccag aaggaaaaac tgagacgatt ctccttctgt gtaatcaagc
     1321 tgtctgtatt tggatgaccg gatcagcgtg gcagagggtg ctatctggat attctgaact
     1381 cttccgcctt tctccgtaag tcctcctgca caggatatat tcagaagaac ggcagacaga
     1441 aataaaatta ccagtctcat atcagtcgaa ttttactgcc tctgcagtaa gccccattct
     1501 gttccatgtg atgaagaaaa aaatcatcct gtcgttccag tagcggatat tgaccagagc
     1561 atctgcattc ttttctttca gagcctcatc gagaaggctg ctgacaggcg gttttttaag
     1621 aggaattccg atgattgcaa aatcaagagt aacccagcct ttttcctttt ccactgagcc
     1681 gatgaccttg tattttcttt cagctatagg gatattgctt gtggcaaaac ctgcatttgc
     1741 agtggcgcag ccgtaagcgg aatatataat cagtaaaact aataatacaa gcttcttcat
     1801 ttgtttcttc ctttgtcgtg ttttgccgat gttctggatt cggaaagtct tttccatttc
     1861 tccatcagac tgactgtgaa acctttttcc agtttcagat actctttcag atccttcatt
     1921 cttttctctg ccttgctgcc ttttttcaga aaacccaggc tttcatccac catcttctca
     1981 aatggtgaat aactttctat tttaattctg agcgaatttt caagtgcaaa gtcggaaaaa
     2041 agtatgtact cctttctgtc tcctgaaatc cttctgtatt ccagaagtcc taccattttc
     2101 agcattttca gattggtgga tacactgctt ctgctgacat tcagaattct ggaaatgtcc
     2161 tcaggagtga ccggttcatc ggctacaagc agcagtccgc agatctgtcc cgcgattctg
     2221 gatatcccca tactttcata ccactgcccc agcccgttga tgaagctgct caattcagaa
     2281 ctgatctgca attctctgtc cactgagact agatttcatt ttctgaaatt tcagtcaaga
     2341 ctgaaactag cgaaatggtt aaaaaatttg tacagggttt tggagataaa gaccggtctg
     2401 tccagtcatc agtttttata ctttgacgcc gatgaggagc atatcgtcaa tctgttcgtg
     2461 atctcccatc cactgactga ttctttcctc aaatatcttt ttctgctcat tcatcggaag
     2521 tgcatggatt tcgagaatga gattcttgaa tctttcctgg gagaacttct ctctgttttc
     2581 tcctccgaac tgatccagat atccgtcagt cgtaatgtaa aagcagagcg ggttttcgat
     2641 gggaagggta tgactttcaa agattctttc ctcttccttc tgcactccgc cgattgactt
     2701 ctttgtgcct ctgattcgga caacggtatc attttctaca taatataatg aatttcttgc
     2761 accggaaaaa ttcagaatct ttctgcggag atcgatcatg cacagagaaa ggtccattcc
     2821 gtctctgctt ttggtattct cctggttcag cgcataccga attgctttat gaagctctga
     2881 aagtatttca gaagggatga gtattctttt ttctcccact gtttcattca ggattgcgct
     2941 tcctatcatg gacatcatgg ctcccggaac cccatgtcct gtacagtcga ttgcagcgat
     3001 cacagcaata ttgtccttct cgtcgcctgt ctgatatttc tggctgtgga aaaaataaaa
     3061 atctccgctg atcacatcct ttggtctgta aagaatgaaa gaatccggaa gaaccgattt
     3121 gacctgctca gaaagaggaa ggattgactt ctgaatcgtt tttgcatatt caatgctttc
     3181 tgtgatattt ctgttctttt cttcaatgat tgacttctga actgatatct cattaaacga
     3241 tttttccagt gcttcatttt tggcactgat ttcgtgcatt gcattctgca gatccttcgt
     3301 tctttcatcg acaagcctgg tcagttccgc ttcccttctt ttcagggtgt ctatcagttc
     3361 actgttctgt atgttcagcc gattaataag gatttttata atccccttga gtatctccgt
     3421 gctgctcccc atgatcctgt aaaaatcggc tgcatccagt ctgagaagaa tgcagtcctc
     3481 ttttgcgatt acagttgcgt ttctcggttc tgagttcaga agagaaaact cccctaaggt
     3541 ctgcatcggg cctacctctg caaatttctg tccttcctta tgtacggata cactgcctga
     3601 atagatgata tacatacagg tgccggggtc tccgatctgt agaacggttc tgccctgtcc
     3661 aaagaactcc tgtttcagtg actgtgcaat ttctgctatt gaattttccg gcgtttcgga
     3721 aaagagggat atttttttca ataccgaaat gatttctaga aggggaagca tggaacagaa
     3781 ttcaagaatt gcatataagt ttctgtccgc aaggattttt ttagtcagca aatgagcaaa
     3841 ttttcagcat ttgtgccgac cgaaaatctg agtctgcatc gcaaagtgca gacagcattt
     3901 ggaaaaagaa gtctgctgac agtcatgaac gggtctgaaa accatgactt cctggataaa
     3961 aggacaaccc cgaactgtga atctgtcagt cgcattatca atga
//
LOCUS       DAOQGJ010000099         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12428_length_3994_cov_2.804773, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000099 DAOQGJ010000000
VERSION     DAOQGJ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12428_length_3994_cov_2.804773"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PK683_02480"
     CDS             <1..1086
                     /locus_tag="PK683_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07342.1"
                     /translation="VPETDRKIFASKTAFVNFHWDSENEEPAVLQVSRNRDFSDLFYS
                     AKSRSGIGMDVPAGQYYWRVSSEGNSSPIRRLNIYNEGPPDLISPKNGRRFDDADRKI
                     SFFWSNNPLSQNYRLDVSRNPDFKERMLSTSLSTNSYTIQRPENGLYYWRVVNFSSDS
                     KRPIQSPISQFSVGNTAPIKEAEEKKPVETVKKEPEKTKEKEEEKKKALEEKKAALEK
                     KKKALEEKKKAAEDRKLAEEKKKAEEMKKDNSLSPNRTTLTLKSREPIVFKWKKLSPD
                     ESYVFQLFNSRTGKLVHSAKGKNNSVSFHKYSLLEEGKFHWEVQRIQGKTKHTSKAGF
                     GISLAKNLQKLKPEEIEIISPDTIYRD"
     gene            1088..2224
                     /locus_tag="PK683_02485"
     CDS             1088..2224
                     /locus_tag="PK683_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07343.1"
                     /translation="MRYITAFFLMIFSLFAEQGYIEWKPIEGTSFYHVEIRNKDRKII
                     TSERTESTRFEVQNPEGSYEFQITPHDLFEKPGISSGWKPLKIIVSRIPELKDNVIVL
                     SPETKFIRLKGKNFLPATKAYVVKEKKHKELSKKYISSEEIDVNLKEPIASQGEFDLV
                     LENPNHKILSRQKYFKIAKKDTEKKPPVKEPAVTVVIPEKTAKKEPEKKPPEKTVVKP
                     DLPKKEIPKKEIPEKTVTVPVKEPEKPPVKITVKPVPEEELKPLSILVTDGIYGMTSH
                     ELNTMIQLEKKACGDSELPHVLIERCFNQHLELNINDRRRKDLYHYIYADSSNYKARM
                     RSYEYFTYNPRYFPALREKLNLRLKDSNIDSEEKIKVVQLLDRI"
     gene            2300..3307
                     /gene="gap"
                     /locus_tag="PK683_02490"
     CDS             2300..3307
                     /gene="gap"
                     /locus_tag="PK683_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNH07344.1"
                     /translation="MTKIAINGFGRIGRLVLRAGLKDPNLEFVAVNDLVTPDNLAYLF
                     KYDSTHGKYQGSVTYDDTNLIIDGKKIQCFSEKEPEKLPWKSLGVDYVVESTGRFTDK
                     ESAEKHIQGGAKRVVISAPAKDKTVPTYVMGVNHEKYNPSTDTVVSNASCTTNCLAPI
                     VKVILDNFGFSEGLMTTVHAMTATQPTVDGPSKKDFRGGRGAAQNIIPSSTGAAKAVA
                     LCIPEVAGKLTGMAFRVPTPDVSVVDLTVKTVKATSLDEIRKKMKEASEGSMKGILGY
                     TDEQVVSNDFIGSAYSSIFDSDACIELNGNFFKLVSWYDNEMGYSNRVLDLIRYMVTK
                     G"
     gene            3490..>3994
                     /locus_tag="PK683_02495"
     CDS             3490..>3994
                     /locus_tag="PK683_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNH07345.1"
                     /translation="MKFFNLIFICTLLLFNAENTEARKLTKEEKKKLREIIQSIGKQK
                     ISYGKKWKPPGSDTELSMDCSNTAKYLYQAPFGVTLPRSAYDQYDLVSKTGGLHTVPT
                     LADKSVDIEKLKKKLKSGDLLFWIDTHSEIPSDRSPSIGHVMIFLGKTKSGKMKMGGA
                     GTFGKGEY"
BASE COUNT         1424 a          761 c          851 g          958 t
ORIGIN      
        1 gttccggaaa ctgaccggaa aatttttgca tccaagacag cttttgtaaa ttttcactgg
       61 gacagcgaaa atgaagaacc tgcagttctg caggtttcca gaaataggga cttctcagat
      121 cttttttatt cagcaaaaag ccgaagcgga atcgggatgg atgttcctgc tggccagtac
      181 tactggcgtg tcagttccga gggaaattcc agcccgatca gaaggctgaa tatttacaat
      241 gaaggtcctc ccgatctgat atctcccaag aatggaagaa gatttgatga tgcggacagg
      301 aagatttctt ttttctggag caacaatccg ctgagtcaga attacagact cgatgtttct
      361 cggaatccgg attttaagga aaggatgctc agtacttccc tcagtacaaa ttcttatacg
      421 attcagagac cggaaaacgg gctttattac tggagagtcg taaatttcag ttcagacagc
      481 aaacgtccga ttcaaagtcc gatttctcag ttcagtgtag gaaatactgc tcctattaaa
      541 gaagcggaag aaaaaaagcc tgttgagacc gtaaaaaagg aacctgagaa gacaaaagag
      601 aaagaagagg aaaagaaaaa agcgctggaa gaaaagaaag ctgctcttga gaaaaaaaaa
      661 aaagcacttg aggaaaaaaa gaaagcagct gaagacagga aactcgctga ggaaaaaaag
      721 aaagcagaag aaatgaaaaa ggacaattcc ctttctccta acagaacgac tctgactctg
      781 aaatccagag aaccgatcgt attcaaatgg aaaaaactga gtccggatga atcctatgta
      841 tttcagcttt tcaattccag aacaggaaaa ctggtgcact ccgcaaaagg aaaaaacaat
      901 tcagtatcct ttcacaagta ttcccttcta gaggaaggaa agtttcactg ggaagttcag
      961 agaattcagg gaaagacaaa acatacatcg aaagcaggct tcggaatttc tcttgcaaag
     1021 aacctgcaga agctgaaacc ggaagaaata gaaattattt ctccagatac aatttacaga
     1081 gactgatatg agatatatta cagcattttt tttaatgatt ttttccttat ttgcggaaca
     1141 gggatacatt gaatggaaac cgatcgaagg cacatctttt tatcatgttg aaatcagaaa
     1201 caaagacaga aagatcatca caagtgaaag gactgaatcg acaagatttg aagttcagaa
     1261 cccggaaggg agctacgaat ttcagatcac gcctcatgat ctttttgaaa aaccgggtat
     1321 cagctcaggc tggaaaccgc tcaagataat tgtttcgagg atacctgaac tcaaggacaa
     1381 tgtcattgtg ctttctcctg aaacaaaatt catcagactg aaaggaaaaa actttcttcc
     1441 cgccactaag gcatatgtgg taaaggaaaa aaagcataag gaactcagta aaaagtacat
     1501 cagctctgaa gaaattgatg taaatctgaa agagccgatt gcatcacagg gtgaatttga
     1561 tctggttctg gaaaatccga atcataagat tctcagcaga cagaagtatt ttaaaatcgc
     1621 aaagaaagat actgagaaaa agccgcctgt aaaagagcct gccgtgacag tggtgattcc
     1681 tgaaaaaaca gcaaagaagg aacctgaaaa aaaacctccg gaaaaaacag tggtaaaacc
     1741 tgacctcccg aaaaaggaaa ttcccaagaa ggaaattccg gaaaaaacgg tcacagtgcc
     1801 tgtcaaagag ccggaaaaac ctcctgtaaa aataacagtt aaacctgttc cggaagaaga
     1861 actcaaacct ctcagcatac ttgtcactga cgggatatac ggtatgactt ctcatgaact
     1921 gaacacaatg attcagctcg aaaagaaagc ctgcggagat tctgaactgc ctcatgtgct
     1981 gattgaaaga tgttttaacc agcatcttga acttaatata aatgacagaa gaagaaaaga
     2041 tctttatcat tatatttatg cagacagttc aaattataaa gccagaatga gatcctatga
     2101 atatttcaca tacaatccca gatactttcc ggcactgaga gagaaattaa atctgagact
     2161 caaagacagt aatatcgatt cagaggaaaa gatcaaagtt gttcagcttt tggacagaat
     2221 ataggaaagt attcgacatt gcattccaca ggtgaaaaaa aggtacaaag aaaattagag
     2281 tacaagacag ggagaaaata tgactaaaat tgctattaat ggttttggaa gaatcggaag
     2341 actcgtgctt cgtgcaggac tcaaagatcc gaatttagaa tttgttgcag taaatgatct
     2401 ggtgacaccg gacaatcttg cttatctgtt taaatacgat tcaacacatg gaaaatatca
     2461 gggatctgtg acttacgatg acacaaatct gatcattgac gggaaaaaaa ttcagtgctt
     2521 ctcagaaaag gaacctgaaa aactcccttg gaaatctctc ggtgtggatt acgtagtaga
     2581 atctacagga agattcacag acaaagaaag cgctgaaaag catattcagg gcggagcaaa
     2641 aagagtggtt atttcagctc ctgcgaaaga caaaacagtt cctacttatg tgatgggagt
     2701 caatcatgaa aaatacaacc cgtccactga cactgtagtt tcaaatgctt cctgtacaac
     2761 aaactgtctt gctcctatag taaaagtgat cctggataat ttcggttttt cagaaggtct
     2821 gatgactaca gttcatgcta tgacggcaac tcagcctaca gttgacggtc catccaaaaa
     2881 agacttcaga ggaggaagag gcgctgctca gaacattatt ccttcatcta caggtgctgc
     2941 caaggccgtt gcactctgta ttcctgaggt tgccggaaaa ctgactggga tggcattcag
     3001 agtccctact ccggacgttt ctgtagtgga tctgacagtc aagacagtaa aagctaccag
     3061 tcttgatgaa atcagaaaaa aaatgaaaga ggcatctgaa ggttcaatga aaggaatttt
     3121 aggatacaca gatgagcagg tcgtttcaaa tgactttatc ggatctgcat attcttccat
     3181 ctttgattcc gatgcatgca ttgaactgaa tggaaacttt ttcaaactcg tatcctggta
     3241 tgacaacgaa atgggatact caaacagagt attggatctg atccgttata tggtgaccaa
     3301 gggctgaaat cagtctgaat aaaaaagcag agggtcttca atgtttagag cccctctgca
     3361 aaacccgcaa aaccagcctt tatataataa atgcaaacga aaatagacat ttttattaaa
     3421 aagaatacta tccgcagtga acagaattcc acagaagcaa atttcgttct gcagaagaca
     3481 ctcagctgaa tgaaattctt caatctcatt ttcatctgca cgcttctgct cttcaatgca
     3541 gaaaacactg aagccagaaa actgacaaag gaagagaaaa agaaactcag agagatcatt
     3601 cagtccatcg ggaaacagaa aatatcctat ggaaagaaat ggaagcctcc tggttctgat
     3661 actgagctgt ctatggactg ctcgaatacc gcaaaatatc tttatcaggc acccttcgga
     3721 gtcactcttc ccaggtctgc ctatgaccag tatgatcttg tatcaaagac aggaggactt
     3781 catacagtac cgacacttgc agacaaatca gtggacattg aaaagctgaa gaagaaactg
     3841 aaaagcggag atcttctttt ctggattgat acacacagtg aaattccttc cgacaggagt
     3901 ccatcgatcg gtcatgtgat gatttttctt ggaaagacaa aatcggggaa gatgaaaatg
     3961 ggcggagccg ggacgttcgg aaaaggtgag taca
//
LOCUS       DAOQGJ010000100         3984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12471_length_3984_cov_2.934334, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000100 DAOQGJ010000000
VERSION     DAOQGJ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12471_length_3984_cov_2.934334"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PK683_02500"
     CDS             <1..830
                     /locus_tag="PK683_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNH07346.1"
                     /translation="YWKTSAKLEFRIYDRRTYEIPPEIDRNLLTHPLLVGFITWQIVE
                     SYEVGLYYVNREFKKILSPNLYYFWQNSGSYPEVRKSDTRIQALDISGQEIMTEEKVT
                     LRLNFTSQYKIKDHLKVHSEIQNLGVSLYTEAQLVLREFISTKKLDDVLQRKDDIGNE
                     ILKILKSKEDVFGVQFISAGIRDIILPGEMKDILNTVLIAEKKAQANLITRREETAST
                     RSLLNTAKLLDDNPTLMKLKELEYLEKILEKIGSVNLSTSSGLLEQLTGLLEKSRQG"
     gene            1245..1778
                     /locus_tag="PK683_02505"
     CDS             1245..1778
                     /locus_tag="PK683_02505"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH07347.1"
                     /translation="MKKLFLLIFVLFSAVSIQAEANPTSSVKKLIGFIRYKKTDSALA
                     MIDTKTYSKNLLKNNYDSLSADQQKEFEEAVKGYIKAKSFPLALNYFSKIDINYEKPV
                     ISGKEAKVPSSILYKGSEKIQFSWVLQEKNGQYLITDFLNDGKLASDTYRTTQVEPVF
                     KNKGFDGLIEAIRKASK"
     gene            1780..>3984
                     /locus_tag="PK683_02510"
     CDS             1780..>3984
                     /locus_tag="PK683_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNH07348.1"
                     /translation="MKKFVSLLCNILLARPVLSAVGLAAVLVLSFLQTKKLTINSNQI
                     DLLPPEFPEVIQTKKVVEMIGGNGFYIIAMKSKDSKGMTDHLYKSYELKKKGKLEEAK
                     KEVELAEKTKQENKEYYDRLEAELKKSADDLYERLTVEPDIRYVSYRYNVSFMQDRLP
                     QYLSPHDLKEVYARIKKKIDFEKEKLNPFYMNLTGEEYNPEFNDIIGKYQRLAKRDIF
                     DEYNISADKGMILVLVKPKGSFVDIEYTRKLDARIKEIVAEMNFDKRGVFVGYTGTYK
                     LTLDDYDSLVNALKPVSIASLLGIILLLVLFFRNPVFIIILTFSLVSGVLITFGLTGF
                     LIGQLNTITSIMAAVLMGLGIDYGIQFLYRFREEYTLREDFLSSVKETIYHTGMASFI
                     SALTTTSAFVVLMFSEFRGFSEFGFIACYGIICIAVCMYFVTAIQIALLLKFIPSSKK
                     IFIIAVKKDEGSSIFERLFANPAKLLKISIVIIIILGMWAPFVKFNYSGRDLLLENQE
                     SLLLYDEIADRFEISSDPQVIVVNTLEESEAVYDFFNPVPKEMESIVDQVVSLWNLAP
                     SKSQQLQNMQLLSRIKEEIKPIQPSMIKEEHQKHIPTAKKYLEVEEFSWEDVPKVFFN
                     QFKEVPESKEKGYMLYVYPKIALWHGKDLIHFYRTVGHFEYPVISKRTLSSLLFAGRE
                     DTELEHEEIKDYQHEKFTEEEERVILESANSMSKAELIKAGILPETAAFITDH"
BASE COUNT         1264 a          756 c          829 g         1135 t
ORIGIN      
        1 cttactggaa aacatctgcg aaactggagt tcagaattta tgacagaaga acatacgaaa
       61 ttcctccgga aatagacaga aatcttctca cgcaccctct tcttgtgggt ttcataacct
      121 ggcagatagt ggaaagttat gaagtcggtt tatattatgt aaacagagag tttaaaaaaa
      181 tattatctcc gaatttatat tatttctggc agaattcagg aagttatcct gaggttagaa
      241 aatccgacac tcgaattcag gcactggata tttcgggtca ggaaataatg acggaagaaa
      301 aggtgactct ccgtctgaac tttacgagcc agtataagat caaggatcac ctgaaagtcc
      361 actcggaaat tcagaatctg ggtgtctctc tttacactga agctcagctg gttctcagag
      421 aattcatcag tactaagaaa ctggatgatg tgcttcagag aaaggatgat atcggaaacg
      481 aaatcctgaa aatactgaaa agcaaggaag atgtttttgg tgtacagttt atctcagccg
      541 ggatacgtga tatcatactt ccgggcgaga tgaaggacat tctgaatact gttctgattg
      601 cggagaaaaa agcacaggca aatctcatta caagaagaga agaaactgcc tccacaagaa
      661 gtcttttgaa cactgcaaaa ctccttgatg acaaccctac tcttatgaaa ctgaaagaac
      721 tggagtatct ggaaaaaatt ctcgagaaaa tcggttcagt caatctcagc acaagttccg
      781 gacttcttga gcagctgacc gggcttttgg agaaaagcag gcagggctga tcatacagcc
      841 tctccacgga aattcttaaa gcaggttgct cccgcccatc ttgaagcggc tgagacctgc
      901 tttccggcct ttctctgtaa attctcttac aatctgtttt cgccgagcag ctgcggcaag
      961 gctcagtata tttactaaat gattggatta tcgactgtct gattggtatt tgacctgctt
     1021 gggaaacgaa tgccagaaaa tcgggcagcc cggaattgac tgaaagatcg gatagctgga
     1081 aacaggaatt tgtaaactga acctgcttta acagcgataa gtcttgtttc cctgcaaaaa
     1141 agaccttttc tgtttttaat tattgtacat cggactcatc ctgtaaaaaa tacttccttg
     1201 caaacccatt actcaaaagg aaattggaaa atctaggggt acacatgaaa aaacttttcc
     1261 ttttgatttt cgtcctgttt tcagcagtaa gcattcaggc tgaagcaaat cctacatcgt
     1321 ctgtaaaaaa actcatcggt tttatccgtt acaagaagac agacagtgct cttgctatga
     1381 ttgatacaaa aacctattct aagaaccttc tgaagaacaa ttatgactct ttgagtgccg
     1441 atcagcagaa ggagttcgag gaagcggtca aaggttacat taaggcaaaa tcctttccat
     1501 tggctctgaa ttatttttcc aaaatcgata tcaattatga aaaacctgtc atttccggaa
     1561 aagaagcaaa agtgccttct tccatccttt acaaaggttc agaaaagatt cagttttcct
     1621 gggtgcttca ggaaaagaat ggtcagtatt tgatcactga ttttctgaat gacggcaagc
     1681 ttgcatccga tacatacaga acgactcagg tggaaccagt gtttaaaaat aagggttttg
     1741 acggacttat tgaagcaatc aggaaggcgt ccaagtaaga tgaaaaaatt tgtttctctg
     1801 ctttgcaata ttctgcttgc ccgccctgtc ttaagtgcag tcgggcttgc agctgtcctg
     1861 gttctttcct ttcttcagac aaaaaaactg actatcaaca gcaatcagat cgatctcctt
     1921 cctcctgaat ttcctgaggt cattcagacc aaaaaagtgg ttgagatgat cggaggaaac
     1981 ggtttctaca tcattgctat gaagtccaaa gattccaaag gaatgacaga tcatttatat
     2041 aaatcatatg aactgaaaaa aaaagggaaa ctggaagaag ccaaaaagga agttgagctc
     2101 gcggaaaaaa caaagcagga aaacaaggaa tattacgaca gacttgaagc tgaactgaaa
     2161 aaatcagcag acgaccttta tgagaggctt actgtagaac cggatataag atatgtatcc
     2221 tatagataca atgtatcctt tatgcaggac agacttccgc aatacttaag tcctcatgat
     2281 ctgaaggaag tgtatgcgag aatcaaaaag aaaatagatt ttgaaaagga aaaactcaat
     2341 cctttttata tgaacctgac aggagaggaa tacaatcctg aatttaatga tattatagga
     2401 aaataccaga gactggcaaa aagagacatt tttgacgagt ataatatttc tgcggacaag
     2461 ggaatgatcc tggttctggt caaaccgaaa ggttctttcg tggatataga atataccagg
     2521 aagctggatg caagaatcaa ggagattgtt gcagaaatga actttgacaa gagaggagtt
     2581 tttgtcggct atacagggac atacaaactc accctggatg attatgacag tcttgtaaat
     2641 gctcttaagc ctgtttccat tgcatctctt ctgggaatca ttcttctgct tgttctgttt
     2701 ttcagaaatc ctgtttttat catcatactt actttctctc ttgtttcagg tgttttgatt
     2761 acatttggcc ttaccggatt cctgattgga cagctgaata caatcacaag tatcatggct
     2821 gcagttctga tgggactggg aattgactac ggaatacagt ttctttacag gttcagggaa
     2881 gaatatactt tacgtgagga ttttctttcc tctgtaaagg aaactattta tcatacagga
     2941 atggcctcat ttatttctgc actgactacc acttccgcat tcgttgttct gatgttttcc
     3001 gaattcagag gattcagcga atttggattc atcgcctgtt atgggattat ctgcattgca
     3061 gtgtgtatgt attttgtcac agcaatacag attgctctgc ttcttaaatt tattccctct
     3121 tcaaagaaaa tctttatcat tgcagtcaaa aaagacgaag ggtcatccat tttcgaaagg
     3181 ctttttgcca atcctgcaaa acttctgaaa ataagcatag tgattatcat tatacttgga
     3241 atgtgggcac cgtttgtaaa attcaattac agcggaagag acctgcttct ggaaaatcag
     3301 gaatcacttc ttctttacga tgaaattgct gacagatttg agatcagttc ggatcctcag
     3361 gtgattgttg taaatactct cgaagagtcg gaggctgttt acgatttttt caaccccgtt
     3421 ccaaaggaga tggagagcat tgtagatcag gtggtttcgc tatggaatct tgctccgtca
     3481 aaatcgcagc agcttcagaa tatgcagctt ctttccagga taaaggagga gatcaagcct
     3541 attcagcctt ctatgatcaa ggaagaacat cagaaacata ttcctacagc caaaaaatat
     3601 ctggaagtgg aagaattttc ctgggaggac gttccgaaag tgttcttcaa tcagttcaag
     3661 gaagttcccg aaagcaagga aaagggttat atgctgtatg tatatcccaa gatagctctt
     3721 tggcacggaa aggatctgat tcatttttac agaactgtag gacatttcga atatcctgtt
     3781 atcagtaaaa gaactctctc ttcgcttctt tttgcaggaa gagaagatac tgaactggaa
     3841 catgaggaaa ttaaagacta tcagcatgaa aaatttacag aagaggaaga aagagtcatt
     3901 cttgaatcgg ccaattctat gagcaaagct gaactcatca aggctggaat tcttcctgaa
     3961 acagctgcat tcatcactga tcac
//
LOCUS       DAOQGJ010000101         3980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12493_length_3980_cov_2.898599, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000101 DAOQGJ010000000
VERSION     DAOQGJ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12493_length_3980_cov_2.898599"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PK683_02515"
     CDS             <1..1063
                     /locus_tag="PK683_02515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07349.1"
                     /translation="QIYGILGCAGVSFVISFSVLGFINKFFPFRVPEENEMQGLNYSE
                     HRATTELSDLFLEMEYQKTTGDLSRNVTVEPFTEVGQIAERYNLVLDKVRSNLREKEE
                     LTKELEMNLKVIQGDLSTAGKIQSGILTEGDRDFGQFHVSVHYIPLTEVGGDFFDIAE
                     IRPGVLRVFLADATGHGVQAALITMAIKTIYESLKKNAVSTNDLLYSLNNEYMSSFGN
                     LSQFFTCAVFDIDADNQRVEYSSAGHIPQILVTKGTVQRMEKTGRIIGVIENSKYAVK
                     SLRFEKQDSMFLFTDGLLEEWNELNEEFGEERLEEILKRDSMPVGKRIEEALLAQKKF
                     RGSIPVQDDITVIGIQNRV"
     gene            1174..>3980
                     /locus_tag="PK683_02520"
     CDS             1174..>3980
                     /locus_tag="PK683_02520"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNH07350.1"
                     /translation="MKDRITSLFKNLFSINLKRTALVTAFFSSLSSFHPLAWNGSFLD
                     RLTGKSCTLEVTLEGTPEYFEELWNYQLIFKNDTVSRMEILRSIKLSPSVEFKESSIS
                     EQGNVMLPMNAWNFSGGKDYEIRIDSLLGEKGCSLSAPLKFAVHIPDRKPYFSIDHYY
                     NVIESGMKQILPVEISNIPEMDLGIAQLDLSSLQKALEKGKNQYQNEVKGLSFVSKKW
                     KTGVQKNGYGRVGLDFSSYLSADKKGWLAVKLGAEETVGNYDYTNSKYTYKPQYTERT
                     MFVQSTDLGITSRTDGDVTHIWVHSIGKALPVSGAELTLYGGKRSFGKCTTDKDGYCE
                     IGSGTSDAKMDSAFIYAVHKASNDSAYMVLSDSKRDYAYGVKEALNAAYFFDRKLYRP
                     GEEVSFKVLLSYRKKGEIEPFSGKNITFTVADSRGQNVLSKTAVTSAEGGADGSYKIP
                     EGAALGHYKVSVKAGAMNETGSDTFQVEEFRPVTFSVNLEAPKTGKAGESLNVSMIGK
                     YLFGAPMSGAKAEYSVQRFSSDISFPEYSDYTFLDGWTFEYYERFSEENGGYYTNGQG
                     ELDAAGRYSFALNLDASGQTVELSDGTKLNLPKPYRLKVEATVKDVDDRSVTNTESIL
                     VSPSDTAIGIRSNDYYRHKDKSFEFDLIALKMNGEKLKGKSAEVYVYKKEWNSVRTGS
                     SGGYSVRNDLQNVLKETKKVQLSSSPLKFSFKAEDSGSYTIVVREKDGNGFAMADFYA
                     YGGKQFVAWDFRSDDSLEIKSDKKTYKPGDKAKIMIKSPFAKARAVVTLEREKVFFRK
                     VMNIEGNSEPIEIPILKEYLPNVHVSVMLFKPRTSPPEGKDISKEDREEFLKEDYGIP
                     AFKSGTLSLTVDTSEKKARLEIASEKKEYSPGDRVKIKIRTEPNAEVALSVADRAVLD
                     IEGYSYSSPLGLFYSHWSNSFS"
BASE COUNT         1209 a          773 c          978 g         1020 t
ORIGIN      
        1 acagatttac ggaattttag gctgtgcggg ggtatctttt gtaatcagct tttccgtatt
       61 aggatttatc aataaatttt tcccattccg tgttcctgaa gaaaatgaaa tgcaggggct
      121 gaattattca gaacaccgtg caactactga gctgtctgat cttttcctgg aaatggagta
      181 ccaaaaaaca actggagatc tgagcaggaa tgtgactgtg gaacctttta cagaggtggg
      241 gcagattgcg gaaagataca atcttgtgct ggacaaggtc agatcgaatc tcagggaaaa
      301 agaggaactc acaaaagaac tggaaatgaa tctgaaagtg attcaggggg atctttccac
      361 tgctgggaag attcagtcag gaattctgac tgagggagac cgcgactttg gacagtttca
      421 tgtatctgtg cactatattc ctctcacaga ggttggagga gatttttttg atatagcgga
      481 aatccgtccc ggggtgctca gggtctttct ggcagatgcg acggggcatg gagttcaggc
      541 tgcactgatc acaatggcaa taaaaactat ttacgaatca ttgaaaaaaa atgcagtttc
      601 aacgaatgat cttctttatt ccctcaacaa tgagtatatg agttccttcg gaaacctcag
      661 tcagtttttc acttgcgcag tatttgatat tgatgctgat aatcaaagag tggaatattc
      721 ttccgccggt catattcctc agatcctcgt gacgaaagga acagttcaga gaatggaaaa
      781 gacaggccga atcattggag tcattgaaaa ttcaaaatac gcagtcaagt ctctgaggtt
      841 tgaaaagcag gattccatgt ttcttttcac tgacggtctt ctcgaagaat ggaatgaact
      901 gaacgaagag ttcggagaag agagactgga ggaaatcctg aaaagggaca gtatgccggt
      961 cggaaaaaga attgaagaag ctcttcttgc acagaaaaaa ttcagaggaa gcatccctgt
     1021 gcaggatgac atcacggtta tcggcataca gaacagagtc tgattctctg aatttactga
     1081 gttttgtccc tcagttgaat ttgctgcctg acaaagccgc accatgccaa atttgtattg
     1141 acctgcacct gcatccgcag atactcagct cctatgaaag atagaatcac atccctgttc
     1201 aaaaatcttt tttcaataaa tctcaaaaga acagctctcg taactgcatt cttttcgagc
     1261 ctctcatcct tccatccgct ggcatggaac ggcagttttc tggacagact gaccggtaaa
     1321 tcctgcactc tggaagtgac gctggaagga acccccgaat atttcgaaga gctctggaac
     1381 taccagctga tttttaaaaa cgacacagtg agcagaatgg agattctgag aagcattaag
     1441 ctttcccctt cagtggaatt caaagagtct tcgatatctg aacagggaaa tgtaatgctt
     1501 cctatgaatg cctggaattt ttccggcgga aaagattatg aaatcaggat agattctctt
     1561 ctgggagaaa agggatgttc tctttctgca cctctgaaat ttgccgttca tattcctgac
     1621 aggaaacctt acttttccat agaccattat tacaatgtaa ttgaatcagg gatgaagcag
     1681 attcttcctg tggagatttc caatattccg gaaatggatc tgggcattgc gcagctggat
     1741 ctctcctctc ttcagaaggc cctcgaaaaa ggaaagaatc agtatcagaa tgaggtcaag
     1801 ggactgtcgt ttgtttccaa aaaatggaag acaggagttc agaaaaacgg gtatggaaga
     1861 gtcggtctgg atttttcctc ctatctttcg gctgataaaa agggatggct tgccgtaaag
     1921 ctgggagctg aagaaactgt aggaaattac gactatacaa acagtaaata cacttataaa
     1981 cctcagtata cagaaaggac aatgtttgtt cagtccactg atctggggat cacttcgagg
     2041 acagacggag atgtgacgca tatctgggtt cattcgatcg gaaaggcgct gcccgtgtcc
     2101 ggggctgagc tgacgctgta cggagggaaa cgttctttcg ggaaatgtac aacggataaa
     2161 gacggatact gtgagatagg aagcggaaca tccgatgcaa agatggattc tgcattcatt
     2221 tatgcggttc acaaagcttc aaacgacagt gcctatatgg tgctttccga ttcaaagaga
     2281 gattatgcgt acggagtgaa ggaagcactg aatgctgcct atttctttga cagaaaactc
     2341 tacaggcccg gtgaggaggt cagtttcaag gttcttctct cgtatagaaa aaaaggggaa
     2401 attgaacctt tttcaggaaa aaatatcaca tttacagtcg ccgattcaag aggacagaat
     2461 gttctttcca aaacggcagt tacttccgct gaaggcgggg ccgacggttc ttataaaatt
     2521 ccggagggag cagcactggg tcattataaa gtatccgtaa aagcgggtgc gatgaatgag
     2581 actggatcgg atacgtttca ggtggaagaa ttcaggccgg taactttttc tgtaaatctg
     2641 gaagctccaa agacaggaaa ggcaggagaa agtctgaatg tatctatgat cggaaaatat
     2701 cttttcggcg ctcctatgtc cggtgcaaag gctgaataca gcgttcagag attttcttct
     2761 gacatcagtt ttcccgagta ttccgactat acttttctcg acggatggac ctttgaatac
     2821 tatgaaagat tttccgaaga aaacggaggg tattatacaa acggacaggg tgagctggat
     2881 gctgcaggca gatacagttt tgctctgaat ctggatgcat caggtcagac ggttgagctt
     2941 tccgacggaa ctaaactgaa tcttcccaag ccgtacagac tgaaagtgga ggccactgta
     3001 aaggatgtgg acgacagatc cgtcacaaac actgaatcca tactggtcag tccgtcagat
     3061 actgccattg ggatacgttc taatgattat tacagacaca aggacaaatc tttcgaattt
     3121 gatctcattg cactgaagat gaacggagag aaactgaaag gaaagtccgc agaagtatat
     3181 gtgtataaaa aggagtggaa ctccgtaagg acaggaagtt ccggaggata cagtgtaaga
     3241 aacgatcttc agaatgtact gaaggagact aagaaagtcc agctttcatc gagtcctctt
     3301 aaattcagtt tcaaggcaga agacagcgga agttatacga ttgtagtccg cgagaaggac
     3361 ggaaacggtt ttgccatggc tgatttttat gcctatggag gaaagcagtt tgttgcctgg
     3421 gatttccggt cagatgattc tctggagatc aaatcggaca agaagaccta caagcccggc
     3481 gataaggcaa aaataatgat aaaatctccc tttgcaaaag ccagagcagt tgtcactctc
     3541 gaaagagaga aagtattctt cagaaaggtg atgaatatag aaggcaattc tgaaccgatt
     3601 gaaattccta tcctgaagga atatctcccg aatgtgcacg tatctgtaat gcttttcaag
     3661 cctagaacat ctccgccgga aggaaaggat atcagcaagg aagacagaga ggagtttctg
     3721 aaggaagact atggcatacc tgcattcaag tcgggaactc tgagtcttac agtggatact
     3781 tcggagaaga aggccagact ggaaatcgcc tcggagaaaa aggaatattc tcccggcgac
     3841 agggtgaaga taaagatcag gacggaaccg aatgcagagg tggctctgtc agtggcggac
     3901 agggcagtac tcgatataga aggctactca tacagcagcc ctcttggact tttttattct
     3961 cattggtcaa attcattttc
//
LOCUS       DAOQGJ010000102         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12648_length_3954_cov_3.002052, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000102 DAOQGJ010000000
VERSION     DAOQGJ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12648_length_3954_cov_3.002052"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1957)
                     /gene="hflX"
                     /locus_tag="PK683_02525"
     CDS             complement(278..1957)
                     /gene="hflX"
                     /locus_tag="PK683_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNH07351.1"
                     /translation="MLQFFIALFSLNTFFSGDSLINKVYGEITALKASQLHRLKALSE
                     KRLKEDIIISADLSRNLCELSVEIGRQIGILIDRMGYISYVMVGDHRSIEIPYLDRLR
                     ISSSRLRGLRLIHTHLYGEPLDEEDLADLILLRLDYITAVVPDENSIPKMYYSAHINP
                     DPNGESPWILADRKFAGQLQTDFLSEIQDLENEISRNMDGLIEAAKQNRAFLVGVYKK
                     NQTKYQISSSLDELKELCRTADIHVVDSFRQIKDSLDPATVLGKGKIREILLKAVQKS
                     VELLVFDLELSPSQAKKISQFCDLKVIDRTQLILDIFARNAQSRDGKLQVELAQLKYL
                     KGRLTELDDNMSRLTGGIGGRGPGETKLEIGKRRVEERISRLEKELESLKKRRELNRK
                     QRKRKDVPVAAIIGYTNAGKSTLLNSLTNSNVTAEDKLFATLDPTTRRVRFPEEREII
                     ISDTVGFIRNLPPELTNAFKATLEELGDADILLHVVDISNPNYEAQIESVENILESLD
                     LKEVKTIRVYNKADAVDPERMIPDDGIAVSAVMKTGFENLLREIEYGVWSL"
     gene            1996..3498
                     /locus_tag="PK683_02530"
     CDS             1996..3498
                     /locus_tag="PK683_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH07352.1"
                     /translation="MLKFFFLIHLFFLCSFSLFSFDRTPKDSAEGKLNIVKSGTDSDD
                     LDDDDSDDDGSPSTAENSNLRGAKTTVDTVRPGTPATSGDWKTSTTQISAMDAEKKRI
                     LTKEGLSSVKLAEEKNNGYSVKQLSRILLSHPIPEVRTEAARALGRMGKGIKSLHKAI
                     DTDGYEVRQSAYRAIERIGSRMSLKYFVSGTKSTDADIRLSSYKGIGKTKSSIGRDLI
                     LRSGIESGEPAIAAAALAGLGYYSRKEDLDIFRRYLSSEVQDHRLGAISGLGNSKHPN
                     SLDILLTALKEFPNLEPEIIFAIMQKKTLASTLALIKIYHGTRNENHQALIQKELNSR
                     KAFGKYAIVKTSTATMKRYSKANSEKVAVLFSSDAARIRKVTDKLFRAKMNGQIIEDK
                     YYLVQAYAVSKDFQKRTLVEGWVFGPKLQIINLNSPTNHKVDYQENYEEDSEDERVYG
                     PSDVKEPPVKPVGTETAPPAKNTTVTPPTKNTTVTTPPAKDDYYDEDEDE"
     gene            3498..3773
                     /locus_tag="PK683_02535"
     CDS             3498..3773
                     /locus_tag="PK683_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNH07353.1"
                     /translation="MAARAVIIAKGKVQGVGFRRFVEDAAINLQLKGYTRNLASGDVE
                     TVAEGEKSLLQSLIEQIKKGSPLSKVTEVEVVWEEFQDEFDDFTVRS"
BASE COUNT         1176 a          867 c          805 g         1106 t
ORIGIN      
        1 gcctgaagtt cctgaattga aattgtctta accatagtat ccgtctcata tttctttgta
       61 tgcagaaaaa aataaagaac ttttttttat ctggaaaaga tgagtgtcct ttgccgattt
      121 actgtcattc tggagtcatc tctgatttct gcggatatta aaatccttaa atcaatccaa
      181 tcgcagattc agattttatt tcgaacaggt tcctgcaatt cctgttccgt ttatatatga
      241 cataatgaat cattcatcct gcaggaattc agatgaatca gagactccag acaccgtatt
      301 ctatttctct gagaaggttt tcaaaaccgg ttttcataac tgcagaaaca gcgattccgt
      361 catccggaat cattctttcc ggatcaactg catcagcctt attgtaaacc cggattgtct
      421 tcacttcctt cagatcaagg ctttcgagaa tattttccac cgattcgatc tgagcctcat
      481 agttcggatt tgaaatatcc accacatgaa ggagaatatc tgcatcaccc agttcttcca
      541 gggtcgcctt gaatgcattt gtcagctcag gcggaaggtt tctgataaag ccgacagtat
      601 cggaaataat aatttccctt tcttcaggaa atctaaccct tctggtcgtc gggtcaaggg
      661 tggcaaaaag tttatcctcg gctgtcacat ttgaatttgt cagagaattc agcaaagtgg
      721 attttcctgc attggtataa ccgatgatcg cagcgacagg aacatctttt cttttcctct
      781 gctttctgtt cagctctctt ctcttcttca ggctttccag ttccttttcc agacgggaaa
      841 ttctttcctc aactctcctt ttcccaattt cgagtttcgt ttcaccgggt cccctgcccc
      901 cgatcccccc tgtcagcctg gacatattgt catcgagttc agtcagtctt cccttcagat
      961 atttcagctg agccagctcg acttgaagtt taccgtctct gctctgcgca tttctcgcaa
     1021 aaatatccag aatcagctga gtgcggtcaa taactttcaa atcgcagaac tgcgaaattt
     1081 tcttcgcctg agacggtgaa agctcaagat caaagaccag aagctcaacg cttttctgta
     1141 ctgccttcag aagaatttcc cggatctttc cttttcccaa aacagttgca ggatcaagag
     1201 agtccttgat ctgacgaaac gaatcgacga catgaatgtc cgctgttctg caaagttctt
     1261 tcagttcatc gagagaactt gaaatttgat actttgtctg atttttcttg tacactccta
     1321 ctaaaaatgc tctgttctgt tttgctgctt caatgaggcc gtccatattc cggctgatct
     1381 cattttccag atcctgaatt tcagaaagaa aatcagtctg gagctgtccg gcaaatttac
     1441 ggtctgcaag aatccacgga ctctcaccgt tcggatcagg attgatatgt gctgaatagt
     1501 acattttagg aatgctgttt tcatcgggta caactgcagt gatataatcc agacgcagaa
     1561 gtatgagatc ggccagatcc tcttcatcca gaggttctcc gtacaaatga gtatgaatga
     1621 gtctgagacc gcgaagtctg ctcgaagata ttctgagcct gtccagataa ggaatctcaa
     1681 tggaacggtg atcaccgacc atgacatagg agatataacc cattcgatca atcagaattc
     1741 caatctgtct tcctatttcg acagacagtt cacataaatt tctcgaaaga tctgcactga
     1801 tgattatgtc ttctttcaga cgtttttcgc tgagtgcctt cagtctgtgg agctgagagg
     1861 ctttaagcgc agtaatttca ccgtaaacct tatttataag actgtctcca gaaaaaaaag
     1921 tgtttaatga aaataatgct atgaaaaatt gaagtatcag gatgtctata ctataaatag
     1981 gattaaggtg ctgttatgct taagtttttc tttctgattc atttgttttt tttatgttcc
     2041 ttttctctct tttcatttga tcggactcca aaagattcag cagaaggaaa gttaaacatt
     2101 gtcaaatcag gaactgacag tgacgatctc gatgatgacg attcagatga tgacggttca
     2161 ccgagcactg cagaaaactc aaatcttcgc ggtgcaaaaa ctacagttga cacagtcaga
     2221 cccggaactc ctgcaacctc aggcgactgg aaaacatcca caactcagat cagtgcaatg
     2281 gatgcagaga aaaagagaat tcttacaaaa gaaggacttt ccagcgtcaa actggctgaa
     2341 gagaaaaata acggctactc ggtaaagcag ctctccagaa ttcttctttc tcatcccatt
     2401 cctgaagtcc gcacggaagc ggcaagagcc ttgggcagaa tgggcaaagg gatcaaatct
     2461 ctgcataaag cgattgatac agacggttat gaagtccgcc aaagtgctta cagggcaatt
     2521 gaaagaatag gttcaagaat gtctctgaaa tatttcgtgt ccgggacaaa atctacagat
     2581 gcagacatca gactctcatc ctacaaagga attggaaaga caaaaagcag catagggaga
     2641 gaccttatac tgagaagcgg catagaatcc ggagaacctg caatagcagc ggctgcactg
     2701 gccggactgg gatactattc cagaaaagaa gacttggaca ttttcagaag atacctttcc
     2761 tcggaagtac aggatcaccg tctaggtgcg atcagcggcc tgggcaattc taaacaccca
     2821 aactctcttg atattcttct gactgcactc aaggaatttc ccaaccttga gccggaaatc
     2881 atctttgcta ttatgcagaa aaaaactctc gccagcactc ttgcattaat taaaatatat
     2941 cacggaacaa gaaatgagaa tcaccaggcg ctgattcaga aagaactgaa cagtaggaaa
     3001 gctttcggga aatatgccat agtaaagact tccactgcaa ctatgaaaag atattcaaag
     3061 gccaattctg aaaaagtggc tgtgcttttc agttctgatg ctgcaagaat cagaaaggta
     3121 actgacaaac tcttccgtgc caaaatgaac ggacagatta ttgaggataa atattatctg
     3181 gttcaggcat atgctgtcag caaggatttt cagaaaagaa cccttgtgga aggctgggtg
     3241 ttcggtccaa aacttcaaat catcaatctg aacagcccga ctaatcataa agttgattat
     3301 caggaaaatt atgaagaaga cagtgaagat gaaagagtct acggtccgtc tgacgttaaa
     3361 gaacctccgg tgaaacctgt cggtacagaa actgcacccc ctgctaaaaa tacgactgtt
     3421 actccgccga ctaaaaacac cactgtaaca actcctccgg caaaggatga ctactacgac
     3481 gaagatgaag atgaataatg gcagcaagag cagtcatcat cgctaaaggc aaggttcagg
     3541 gagtgggttt tcgaaggttc gtggaagatg cggccataaa ccttcagctc aaaggatata
     3601 ctagaaatct tgcctcagga gatgtagaaa ctgttgcaga aggagaaaaa tcacttctgc
     3661 aaagcctgat tgaacaaatt aaaaaaggct ctcctctttc aaaggtgaca gaagtagagg
     3721 ttgtctggga agaatttcag gatgaatttg acgattttac agttagatcc tgatttcagg
     3781 tttattcagt tatccattcc gaaacattct tcgaattaaa cattcgatat ggaaaccttt
     3841 ttgagaggtc tttacaatat ctgataactc accttacgcc tgcgggcaat gctgtcaagt
     3901 taaggttctt tattcaggaa agacggttgc cgagtgattt tcaccgccca cctc
//
LOCUS       DAOQGJ010000103         3933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12753_length_3933_cov_3.401238, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000103 DAOQGJ010000000
VERSION     DAOQGJ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12753_length_3933_cov_3.401238"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1512
                     /gene="rpoD"
                     /locus_tag="PK683_02540"
                     /pseudo
     CDS             22..1512
                     /gene="rpoD"
                     /locus_tag="PK683_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            1682..2872
                     /locus_tag="PK683_02545"
     CDS             1682..2872
                     /locus_tag="PK683_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNH07354.1"
                     /translation="MKDIDITLYGATGFTGKLTAEYLLGRKKESDFSFAIAGRSRSRL
                     EELKSDLLKRFPSAGNIEIIIADSDNYESIRRMAERSRVVVTTVGPYLKYGEPVLRAC
                     AEAGTHYTDLTGEGGFVSSMSEKYESAAIANKAKILNCCGYDSIPADLGTYFLMKNFG
                     PDEDVEVECFISFGSKADDSFSFLKSFSGGTWHSALGFMNMEELDRRKVSFQKISASV
                     SGRDIGVIPTEFRFREDTRTWGMPLPFVDEEVVLRSAAKNPSYGKSFRYGHYAGISST
                     PLLFGVLLGAGAVMTLAQFSLTKKFLYSLKSPGDGPDEHTRNTNSFCTSIVGTSKTKQ
                     RKVEVRGGDPGYGDTAKMLGETALCLLKDELPEVYGFQTPASACGDRLLERLNKAGIS
                     FIQI"
     gene            complement(2859..>3933)
                     /locus_tag="PK683_02550"
     CDS             complement(2859..>3933)
                     /locus_tag="PK683_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07355.1"
                     /translation="IILCLISGIITVSLNVWYYSSSRYSVGLGTKFVDEGFAAVPNRF
                     FIFGRFLFQLLIPFRPSITSYDPSSWLSVIGIILLPLFIFAVWKLNENRKQIIVWSFF
                     TLLPLITVNGPSNQHFGWDTYLLTPLLGWTMILGLTLRNISEKYNASDRNLLIIILSF
                     ILIFSVIAKTASEAWKDDESLWKRAMETEGSAAAMAGYIKTSLSLKKNVHKLWPNALE
                     LQKKQPNDQDIPYIFGRMIFEDPELNRNEKEELFAKYEMKSPWFVYYRSAFDASDRRF
                     TDADRRMSELFQNDPGKTALYFRDRLPEISASWYAMCVFAKKSTCQENIRQIRRSLPP
                     GRWNEEEFSKRLSSFRLAPFKSE"
BASE COUNT         1248 a          754 c          916 g         1015 t
ORIGIN      
        1 atctctttca gataaagtct gagtgcatct tcggaagatc ctatcagact ttatctgaaa
       61 gagattggaa aggtcagcct gatttccgga gagacagaag tctttcttgc caaaagaatt
      121 gagaagggcg agaagatcat tgaagaaaca attctgggtt caagcattct gagagccact
      181 tttgcaaaac tgatgccgaa ggtacgaaac aagaaaatcc gtatctggga actgatcaga
      241 gtagacaagc tttatgcaat gaatcaggca gaagcggaaa agctcgaaaa acttttcttt
      301 cagaatatgg aagcgattca gtcggaagaa agagttctgg ccgaggctct gaaccggatt
      361 aagaaatatt ctgagaacag caagaagttc aaagaactga aagaaaagat agatgcatct
      421 gctgccaaaa tagataaatg cgtccgcgaa ataggcgtat ctcagagaga aatccagaag
      481 atttcacaga agatcaaatc aatggttttc agaatcaagg aaatagacag acatttcctt
      541 aaaatcaagg caagatacgg tcatgacgtc agagagatca agacattcaa caggttcatt
      601 gagaagaatg aaaatctgga cgagatcgaa aaaatgatgg gcgttcatat tgacgaggtc
      661 cgtgaagtta tcaaggacat acggaataat gaaagaaaac tcagacgtat ggaacaggaa
      721 gccggctctc ccacaaatga aatcaaggag tggggagaaa aaatcattaa gggtgaaaga
      781 gaaatatccc aggccaagaa agaacttgtg aaggccaatc tccgtctcgt cgtttccatt
      841 gccaaaagat atgccaacag agggatgcac ttctttgatc tgattcagga aggaaacatc
      901 ggccttatca aggcagtgga caaattcgaa tataaaaaag gttataaatt ttccacttat
      961 gcaacctggt ggatacgtca ggcgattacc agagccatct ccgatcaggc aaggacgatc
     1021 cgtgttccag tgcatatgat tgaacaggtc aacaaagtga tcagagaggc aagactgttt
     1081 gtgcaggaat tcggaagaga ccctacaaat gaagaaattg cagaaagact cggatggcct
     1141 gttcagaaag tcaagtcggt caagaatgta ggacgtgaac cgatatccct tgagattcct
     1201 atcggaagcg aggaagattc tgagcttggc gatttcattg aagacaagga tgttgaatct
     1261 cctctgaatt ctgcagcgtc tacaattctt gcagagcaga tccgtcaggt tcttcatact
     1321 cttcctgcca gagagcagaa agtcatccgt atgcgttttg gactcgatga cgggtatgcg
     1381 cagacacttg aggaagtagg ttatcagttc aaggtgacaa gagaacgtat acgtcagatt
     1441 gaggcaaagg ctctcagaag actgcgtcac ccaagcagat ccaagaagct gagagattat
     1501 atagttgagt agcaatttat tcaatattta tttttatcgg gaaattacgg gattttcaag
     1561 ggcgaagccc ttgaaaccgt cggtagaccc taaaatacca gcagctgcga agccgtaagg
     1621 ctgagcagct tcgaattatc ttgagattct gcatttagga agtcagaagc ggggagcagc
     1681 aatgaaagac attgatatca cactttacgg tgcaacaggg tttacaggga aactgactgc
     1741 agaatatctg cttggcagga agaaagaatc tgatttcagt tttgcaattg caggaagaag
     1801 cagaagccgg cttgaagaat tgaagtctga ccttttgaaa cgattccctt ctgccggaaa
     1861 tattgaaatc atcattgctg acagtgataa ttacgaaagt attcgcagga tggcagaaag
     1921 aagcagagtt gtagtgacca ctgtaggccc ttatctgaaa tacggtgagc ctgtgcttcg
     1981 tgcctgtgca gaagcaggga ctcattatac tgatcttaca ggagagggcg gatttgtatc
     2041 ttccatgagc gagaaatatg aatctgccgc catcgcgaac aaggctaaaa tactgaactg
     2101 ctgcggatat gacagtattc ctgccgactt gggaacatat tttttaatga aaaacttcgg
     2161 tcctgatgag gacgttgaag tggaatgttt tatcagcttc ggatcaaaag cagatgattc
     2221 tttttccttt ctgaagagtt tttccggagg gacctggcac agtgcattag gttttatgaa
     2281 tatggaagaa ctggacagaa gaaaggttag ttttcagaaa atttccgctt ctgtttccgg
     2341 aagagatatc ggagtcattc cgactgaatt ccgtttcaga gaagatacaa gaacttgggg
     2401 aatgcctctt cctttcgttg atgaagaagt tgtattaaga tcggccgcaa aaaacccttc
     2461 ctatggaaaa tcattccgtt acggtcatta tgcaggaatt tcttccacac ctctcctgtt
     2521 tggagtgctt cttggtgcag gagctgtaat gactctggca cagttcagtc tgactaagaa
     2581 atttctctac agtctgaaaa gtccaggcga cggtccagac gagcatacaa ggaatacaaa
     2641 ttcattctgc acaagtattg tcggaacttc aaaaaccaag cagagaaaag tggaagtcag
     2701 aggaggagat ccaggatacg gcgatacagc aaaaatgctc ggtgagactg ctctctgtct
     2761 tctgaaggat gaacttccgg aggtttacgg atttcagact ccggcatctg cctgcggtga
     2821 cagacttctg gaaagactga acaaggctgg aatttctttt attcagattt aaaaggtgcg
     2881 agcctgaaag aggaaagcct tttactgaat tcctcttcat tccatctgcc tggcggaaga
     2941 gatcttctga tctgacggat attttcctgg caggtgcttt ttttggcaaa tacgcacatt
     3001 gcataccagg aggcagaaat ttcaggaagc ctgtctctga aatacagagc tgtttttcct
     3061 ggatcattct gaaataattc actcattctt ctgtcagcat ccgtaaaacg tctgtctgat
     3121 gcatcgaatg cagatctgta atacacaaac catggactct tcatttcata ttttgcaaac
     3181 agttcttcct tttcattccg attcagttca ggatcttcaa agatcattct cccgaatata
     3241 tatgggatat cctgatcatt cggctgtttt ttctgaagtt ccagagcatt cggccagagt
     3301 ttatgcacat tttttttcag actcagactg gttttaatat atcctgccat tgcagcagct
     3361 gaaccttctg tttccattgc tcttttccag agggactcat catctttcca ggcttcagaa
     3421 gctgtttttg ctatgactga aaagattaaa ataaatgata aaataatgat cagtaaattt
     3481 ctgtccgaag cattgtattt ttcactgata ttcctcagtg tcagtccaag gatcatagtc
     3541 cagccgagga gaggtgtcag aagatatgtg tcccagccga aatgctgatt ggacggaccg
     3601 tttacagtga tcagaggaag gagagtaaaa aaactccaga caatgatttg ttttctgttt
     3661 tcattcagct tccacacagc aaaaataaaa agaggaagaa gaataattcc gatcactgaa
     3721 agccaggagg aaggatcgta ggaagtgatg gaaggacgga aaggaataag aagctgaaac
     3781 agaaatcgtc caaaaataaa aaaccggttc ggaactgcag caaaaccttc gtccacaaat
     3841 tttgttccca gcccgactga atatcttgaa cttgaatagt accatacatt caatgataca
     3901 gtaatgattc cagaaataag acagagaatt atc
//
LOCUS       DAOQGJ010000104         3920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12812_length_3920_cov_3.224321, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000104 DAOQGJ010000000
VERSION     DAOQGJ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12812_length_3920_cov_3.224321"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..209)
                     /locus_tag="PK683_02555"
     CDS             complement(57..209)
                     /locus_tag="PK683_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07356.1"
                     /translation="MMKLLILILLLSIGYHYGIRLGINFDELFDFFFGIVGIDFKEDD
                     IGWQKD"
     gene            complement(361..1515)
                     /locus_tag="PK683_02560"
     CDS             complement(361..1515)
                     /locus_tag="PK683_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07357.1"
                     /translation="MNLKEYQSKVLKGQNLPADLKILLQMQDFRIESEEDVSDPLSEM
                     GIYLLLPGDDPLPDDSHLSKKEKNTPFYKANYPAVMSVMKLITFAAQIIYDDGDEDPV
                     GYWHGPENTPIEEAPIVKWSSDGFTVLPGKNLTEALLTTLDEEDDFPRIRKSLSEYGI
                     RISARKISEIKTPKVKTHPNELHGILYSDNRILSFQKDVLNGQTVPDDLRKLFRMQML
                     WPEEENSEIYDPLGAMNCRILIPGEKIGVMDKSYLSKADKKNADIMANVKSIDKVCSY
                     ITFAAYTDDGDLIGYWHGTENNSIEKAPIVMLDTEGQFSQLPGKNLTEALLSLQDMNN
                     SEFKKYAKWFAEFHILITANSQRGMTQWPKSASSPQKIHQDLYEKYRRKK"
     gene            complement(1675..3039)
                     /locus_tag="PK683_02565"
     CDS             complement(1675..3039)
                     /locus_tag="PK683_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNH07358.1"
                     /translation="MENKIILTENEIPRNWYNIQADMPNPVLPPLHPGTKQPVGPDDL
                     APLFPMELIKQEVTRERYVEIPEEVRDIYKIWRPTPLFRAHRLEKFLDTPAKIYYKYE
                     GVSPAGSHKPNTAVPQAYYNKKEGVKRITTETGAGQWGSALSFACNIFGIELEVYMVK
                     ISYQQKPYRKAMMNTWGAKVYASPTDRTESGRKILAGDPNSPGSLGIAISEAVERAAM
                     DPDTKYALGSVLNHVLLHQTIVGQEAMLQMEKAGDLPDIVIAPFGGGSNFAGLTFPFL
                     RLNFTEGKKIRCIAVEPASCPKLTKGEFRYDFGDTVGLTPLIPMYTLGHSFVPASIHA
                     GGLRYHGAGAIVSQLLKDKLIEASALQQLECFEAGVAFAKTEGILPAPEATHGIAEVF
                     RQAKQAKIEGKSKTILFNLCGHGYLDLGAYEDYLSGKLKDHEISDTEVKKYISELDAY
                     PAIK"
     gene            complement(3153..>3920)
                     /locus_tag="PK683_02570"
     CDS             complement(3153..>3920)
                     /locus_tag="PK683_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNH07359.1"
                     /translation="KIEISGDVPSSLRESIHLKLKSAIMEKYGKNYSIVGQTDLEVLF
                     RQAESFQKQGKEIQTFLTEISGAKDADEIIYGKLFKDEGQIKLLLNNLKKDSVTKEFY
                     TKSIVDISFFDSDSDFYIKEAAEKIMNPRHKILHTGPSSPDHKKVYHPKSEYDFPPEN
                     KIFQVGDLEWGEFEGVFKWREATVICESKGMRLPTPEELKNLAHLKNYLTREPCCVFW
                     TSRTNAKDGDYAFYIDINDGFGNYYHKDIDLRVRCVK"
BASE COUNT         1010 a          859 c          805 g         1246 t
ORIGIN      
        1 cctgaaagac tatttcccga tcgactggtg agacagaaaa taaaataaat tatatttcag
       61 tctttctgcc agccgatatc atcttccttg aaatcaattc cgacgattcc aaaaaagaag
      121 tcaaagagtt catcaaaatt gattcccagt ctgattccgt aatgataccc aatcgaaagt
      181 aagagtatca ggatcagcag tttcatcatt ttcagaaaaa taaccgcgaa gcattgtcat
      241 tcaaaaattt tgcgttcatt ttatagtctc cttgtgatta gactgatgca gaccgagttt
      301 cttctgaaaa aacaataggc agtcctgagt cattccccac agtttgaact gaagaaaacc
      361 tcattttttt ctgcggtatt tttcataaag atcttgatga attttctgag gagacgatgc
      421 agatttgggc cactgagtca taccgcgttg cgaatttgca gtaattaaaa tatgaaattc
      481 agcaaaccat tttgcatatt ttttaaactc gctgttattc atatcctgaa gactgaggag
      541 cgcctctgtc aaatttttcc cgggcaattg actgaactgt ccttctgtat caagcatgac
      601 aatgggggct ttttcgattg aattgttttc agtgccgtgc cagtatccga ttaaatcgcc
      661 gtcatccgtg taagctgcaa aagtaatata ggaacatact ttgtctattg acttcacatt
      721 tgccattata tcagcatttt ttttatccgc tttactgaga taagatttgt ccataacgcc
      781 gattttttcc ccaggtatta aaattctgca gttcattgcc ccaagggggt cgtagatttc
      841 agaattttcc tcctccggcc aaagcatctg cattctgaac agtttacgaa gatcatctgg
      901 aacagtctgt ccattcagta catccttctg aaaactgagt atccgattat ccgaatataa
      961 aattccatgc agttcatttg gatgagtttt tactttcggt gttttaattt cagaaatctt
     1021 ccttgcactg atccttattc cgtattcaga gagactcttc cggattcttg gaaaatcatc
     1081 ttcttcatcc agtgttgtaa gaagcgcctc agtcagattt tttccaggca ggacagtaaa
     1141 accgtcactt gaccatttta caatgggagc ctcctcaatg ggcgtgtttt ctggtccatg
     1201 ccagtaaccc actggatctt catcgccgtc atcgtaaatg atctgagcag caaaagtgat
     1261 caacttcatc acgctcatca cggcaggata atttgcttta taaaaaggag tgtttttttc
     1321 ttttttactg agatgtgaat catccggcaa tgggtcatcg ccaggaagca gaagatagat
     1381 tcccatttca ctcaaaggat cagatacatc ttcctcgctt tctattctga aatcctgcat
     1441 ctgcagaaga attttcaagt ccgcaggaag gttctgacct ttcagcactt ttgactgata
     1501 ttctttaagg ttcataaggt tctctttttt tcataaaagt atgataaaaa tgccgcttcc
     1561 agttaaaatg aattttctgt tgtgtaaagg aattatttcc ttcattgcag tcagatccga
     1621 ctccttgaat ccgttgaaag caattcccgg agtcggaata atttttgtgc aatattactt
     1681 gattgcagga taagcatcca gttcagaaat gtattttttc acttctgtat cgctgatttc
     1741 atgatccttc agttttccgg aaagatagtc ttcatatgct cccagatcca gatatccgtg
     1801 accgcagaga ttgaagagga ttgttttaga ctttccttct atcttcgcct gttttgcctg
     1861 acggaacact tctgcaatgc cgtgtgttgc ttcaggtgca ggaagaattc cttctgtctt
     1921 tgcaaatgca acaccagctt caaaacattc cagctgctgg agtgcactcg cttcaatcag
     1981 tttatccttg aggagctgac ttacgattgc gcctgcaccg tggtagcgga gtcctcccgc
     2041 atgaatggaa gcagggacga aggagtgacc tagagtgtac atagggatga gaggagtgag
     2101 tcctactgtg tcgccgaaat catagcggaa ttcgcccttt gtgagtttag gacaggaggc
     2161 aggttcgact gcaatacagc gtattttttt cccttcagta aaattcagac gcaggaaagg
     2221 gaaggtgagt cctgcaaaat tcgatcctcc tccgaatgga gcaatgacga tatccggaag
     2281 gtctcctgcc ttttccatct gaagcattgc ttcctgtcct acgatggtct ggtgcagaag
     2341 aacgtgattc agaacgcttc caagggcata ttttgtatca gggtccattg cagctctttc
     2401 cactgcttcg gaaattgcaa ttcccagact tccaggagag ttcggatcgc ctgccagaat
     2461 ctttcttcct gattctgtcc tgtcagtagg agatgcatat acctttgcac cccaggtatt
     2521 catcattgcc tttctgtaag gcttctgctg ataactgatc ttcaccatgt acacttcgag
     2581 ttcaatcccg aatatattgc atgcaaaact cagtgcgctt ccccattgtc ccgcaccggt
     2641 ttctgtagtg attcttttca cgccttcttt cttgttatag taagcctgag gcactgcagt
     2701 gttcggttta tgagagcctg caggacttac tccctcatac ttataatata tttttgcagg
     2761 tgtatccaga aatttttcca gtctgtgagc acggaaaagc ggggtaggtc tccagatttt
     2821 atagatgtct ctgacttctt cgggaatctc cacgtatctt tccctggtca cttcctgctt
     2881 gatcagttcc attggaaaaa gaggagcaag atcgtcaggg cctacaggct gctttgtgcc
     2941 cggatgcaga ggaggcagaa ccgggttcgg catatccgcc tgtatattgt accagttcct
     3001 ggggatttca ttttcagtaa gaatgatttt gttttccata tgctgatatt taaagaattg
     3061 atttcaatgg aaagaagaaa aataattgag tcggcttgag gagaaatttg actgctctca
     3121 gcgaacgatt ttgagcctca atacggataa atctatttta cacagcgtac ccgaagatcg
     3181 atatccttgt ggtagtaatt tccaaagccg tcattgatgt ctatgtagaa tgcatagtct
     3241 ccgtcctttg catttgtcct ggaagtccag aacacacagc agggctctct tgtcagatag
     3301 tttttcagat gagccagatt tttcagttct tccggcgtag gcagtctcat ccccttgctt
     3361 tcgcagatca ctgtggcttc tctccatttg aatacccctt caaactcgcc ccattccaga
     3421 tcgcctacct gaaagatttt gttttccgga ggaaaatcat attcggattt cggatgatat
     3481 acctttttat gatccggaga agaaggaccg gtgtgcagta tcttatgacg gggattcatg
     3541 atcttttccg cggcttcctt tatgtaaaaa tcggaatcgg agtcgaagaa ggaaatatcc
     3601 acgatggact ttgtataaaa ctcttttgta acggagtctt tcttcagatt gttcagaaga
     3661 agttttatct gtccttcatc cttgaagagt ttgccataaa tgatttcatc tgcatccttt
     3721 gcccccgaaa tttcagtgag aaaggtctgt atttcctttc cctgtttctg aaatgactcg
     3781 gcctgacgga aaaggacttc cagatccgtc tggcctacaa tgctgtagtt tttgccgtat
     3841 ttttccatga tggcagactt gagtttcaga tgaatgcttt cccggagaga ggacggaaca
     3901 tcccctgaga tttcaatttt
//
LOCUS       DAOQGJ010000105         3895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12951_length_3895_cov_2.881510, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000105 DAOQGJ010000000
VERSION     DAOQGJ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12951_length_3895_cov_2.881510"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..461
                     /gene="groES"
                     /locus_tag="PK683_02575"
     CDS             177..461
                     /gene="groES"
                     /locus_tag="PK683_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNH07360.1"
                     /translation="MAIKPLGDRVLVEPKKEGEEKIGSLYVPDTAKEKPQEGTVVAVG
                     SGRYEDGKLVALEVKVGDKVLYGKYSGTEIKQGGKEYLIVRESDILAVIQ"
     gene            473..2119
                     /gene="groL"
                     /locus_tag="PK683_02580"
     CDS             473..2119
                     /gene="groL"
                     /locus_tag="PK683_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751305.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNH07361.1"
                     /translation="MAKSIEYNEEARRKLLSGVNKLANAVKVTLGPKGRNVVIDKKFG
                     SPTITKDGVTVAKEIELEDSIENMGAQMVKEVSTKTNDIAGDGTTTATILAQAIITEG
                     LKNVTAGANPMALKHGIDKAVSAAVEHIKKKAVKIENKQDIANVATISANNDKEIGNL
                     IADAMDKVGKDGVITVEEAKSIETTLDVVEGMQFDRGYISPYMVTDAEAMVATLSEPF
                     ILIYDKKISSMKDLLPVLEKVAQSGRPLLIIAEEVEGEALATIVVNTLRKTISCVAVK
                     APGFGDRRKAMLEDIAVLTGGQVVSEDLGMKLENADLKTLGKANKVVIDKENTTIIEG
                     KGSSKDIQGRISQIKKQIEDTTSEYDREKLQERLAKLAGGVAVIHVGAATEVEMKEKK
                     ARVEDALSATRSAVEEGIVPGGGLTLLRAQDSVKALKLEGDESTGAKIVLRALEEPVR
                     MISNNAGMEGSVVVEEARKKEGNIGFNALTMVWEDMLKAGIVDPAKVVRSALQNAASI
                     GSMILTTEVTITDIPDKKDSMPAGGGMGGMGGMGGMGGMM"
     gene            2461..2676
                     /locus_tag="PK683_02585"
     CDS             2461..2676
                     /locus_tag="PK683_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07362.1"
                     /translation="MGVIRLRQTTDSDGNLALSIPTIQKNCTVEIVIVITPYEIKPQK
                     PILEKYLGKLQWEGDALSEQRRLRNEW"
     gene            2718..3059
                     /locus_tag="PK683_02590"
     CDS             2718..3059
                     /locus_tag="PK683_02590"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNH07363.1"
                     /translation="MNTAKYLSEKEWEVIQLYKNYLLKNYSENIKNFYLFGSKARGDF
                     REDSDIDILITLNDYNWRLGDEIRRVGYELDEEIDSKFSILVCSDSEFGNWMNKRYQF
                     VENVMKEGVAI"
     gene            3059..3436
                     /locus_tag="PK683_02595"
     CDS             3059..3436
                     /locus_tag="PK683_02595"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNH07364.1"
                     /translation="MSEEEVKDEMGRANKALKSAEILYKEKLYEDSISRSYYAVLHAA
                     KAALLSRGIEVDSHEAVKRLFGLHIIKTGISEKYYGLILREEQDDRMMADYNVGFSPE
                     EERVFQRLEDARDFVSKMESIIG"
     gene            3456..>3895
                     /locus_tag="PK683_02600"
     CDS             3456..>3895
                     /locus_tag="PK683_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07365.1"
                     /translation="MDNKFANGSEWRKWDLHIHTPVSLCSEYGGQTDDVWEKFISEIE
                     RLSESIKVIGINDYIFIDGYKKVLDYKNKGRLKNIDLILPVVELRLKEFVGNADLNRI
                     NYHIIFADESILAIDNIEAQFLSGLKGKANLDSDSSSGYTWGGVV"
BASE COUNT         1347 a          651 c          905 g          992 t
ORIGIN      
        1 ccgccatccc acaaaacccc tgattttatt gagttttatt catttttctt cctgaaaaat
       61 ccccaaatta gcactctcga ttaaagagtg cttgactttt tttgacccct gaaaaaggat
      121 ggtactcgaa attagcactc caaaaacgat agtgctaaag ttggatggag gtaaaaatgg
      181 ctattaaacc acttggagac agagttctcg ttgagcctaa aaaagaaggc gaagaaaaaa
      241 tcggcagtct gtatgtacct gatactgcaa aagaaaaacc gcaggaagga actgttgtag
      301 cagtcggaag cggacgttat gaagacggaa agcttgtggc tctggaagtc aaagtcggag
      361 acaaggttct gtacggaaaa tattccggaa ctgagatcaa gcagggcggg aaagaatatc
      421 ttatcgttcg tgaaagcgac atactcgctg taattcaata aggagacaaa atatggcaaa
      481 atctatagaa tataatgaag aagcacgcag aaaacttctc tcaggcgtaa acaagcttgc
      541 taacgctgta aaggttacac tcggaccaaa gggaagaaac gtagttattg ataaaaaatt
      601 cggttctcct acaattacta aagacggtgt tacagtagca aaagaaatcg agctcgaaga
      661 ctccattgaa aatatgggag ctcagatggt caaagaagtt tccaccaaga caaatgacat
      721 cgcaggtgac ggaactacta cagctacaat tcttgcacag gcaatcatca ctgaaggtct
      781 gaagaacgtg actgccggtg caaacccaat ggctctcaag cacgggatcg acaaggctgt
      841 ttcagctgct gttgagcaca tcaagaaaaa agcagtcaag attgaaaaca agcaggacat
      901 cgcaaacgtt gcaacaatct ctgcaaacaa cgacaaagaa atcggaaatc tgattgctga
      961 tgcaatggac aaagtcggaa aagacggagt catcactgtg gaagaagcta agtccatcga
     1021 aactacactt gatgtagtag aaggtatgca gttcgacaga ggatacattt ctccttatat
     1081 ggtgactgat gcagaagcta tggttgcaac tctcagcgaa ccattcatcc tgatctacga
     1141 caaaaagatt tcttcaatga aagatcttct ccctgttctc gaaaaagtag ctcagagcgg
     1201 aagacctctt ctcatcatcg ctgaagaagt agaaggcgaa gctcttgcaa ctatcgttgt
     1261 aaacactctg cgcaagacta tttcctgtgt tgctgtaaaa gctccaggat tcggcgacag
     1321 aagaaaagct atgctcgaag acatcgcagt tctgaccggc ggacaggttg tttctgaaga
     1381 cctcggaatg aaactcgaaa atgctgatct gaaaacatta ggaaaagcta ataaagttgt
     1441 catcgacaaa gaaaacacaa cgatcatcga aggaaaaggt tcttccaaag acattcaggg
     1501 aagaatttct cagatcaaaa aacagatcga agacactact tctgaatacg acagagaaaa
     1561 acttcaggaa agacttgcta aacttgcagg cggtgttgcc gttatccatg tcggcgctgc
     1621 gactgaagtg gaaatgaaag aaaagaaagc cagagtggaa gacgctcttt ctgcaactcg
     1681 ttctgcagtg gaagaaggaa ttgttccagg aggcggtctg actcttctcc gtgctcagga
     1741 ttccgtaaag gcactgaaac tcgaaggcga cgaatctact ggagcaaaaa tcgttctgcg
     1801 tgctcttgaa gaaccagttc gtatgatctc caacaatgca ggaatggaag gttctgtagt
     1861 tgtggaagaa gctcgcaaga aagaaggaaa catcggattc aacgctctga ctatggtatg
     1921 ggaagatatg ctgaaagccg gtatcgttga ccctgcaaaa gtggttcgtt ctgctcttca
     1981 gaatgctgct tccatcggtt caatgatcct cactactgaa gtgactatca ctgatatccc
     2041 agacaagaaa gattctatgc cagccggcgg tggaatgggc ggtatgggcg gaatgggagg
     2101 catgggcgga atgatgtaat catttcgaac atgtttctcc gtagcagtcg gatatatccg
     2161 actgctacag tgttattgca ccaaaccaaa agcctcgtcg gaaacggcgg ggcttttttg
     2221 ttttaggaga gggaaccaca gggaagaggg aatatgacga aagactgggt tttatgttaa
     2281 aaatgagatt aaaatatgaa aaagcgtgac gaaattgcct taagaaattt atttgagtaa
     2341 gggctttatt cggatgcaga acttataaca attggaaaag aaatgagcaa agaatgagaa
     2401 ttgacaagcg gataaaattg ataaacttat aagtatctga cttttgggag gattactgca
     2461 atgggagtca ttcgattaag acaaacaact gattccgatg ggaacctggc attatccatt
     2521 ccgaccattc agaaaaactg tacggtagaa attgtaattg tgattactcc atatgaaata
     2581 aaacctcaaa aaccaatctt ggaaaagtat ttaggaaaac ttcagtggga aggagatgct
     2641 ctctctgaac agaggaggct caggaatgaa tggtaatttt ctattcgata caaatgcata
     2701 aaatcaggag ggattctatg aacactgcaa agtatttgag tgaaaaggaa tgggaagtga
     2761 ttcaattgta taaaaattat cttctgaaaa attattccga aaatattaag aacttttatt
     2821 tattcggctc taaggctagg ggagattttc gagaagattc tgacattgat attttgatta
     2881 cattgaatga ttacaactgg agattaggag atgagatcag gagagtaggt tatgagttag
     2941 atgaagagat agattccaag ttttccatac tcgtctgctc cgactcagaa tttggaaatt
     3001 ggatgaataa gagatatcag tttgtagaga atgtaatgaa agaaggtgtt gcaatctaat
     3061 gagcgaagaa gaggttaaag atgagatggg cagagcaaat aaggctttaa aatcagccga
     3121 aatactgtat aaagagaaat tgtatgaaga tagcatttcc agaagttact atgcagtgtt
     3181 acatgcagca aaagcagcct tactgtcaag aggaatagag gtggattccc atgaagctgt
     3241 taaaaggctt tttggcctac acattattaa aacaggaata tctgaaaaat attatggact
     3301 gatattacga gaagagcagg acgatcgaat gatggcagat tataatgtcg gcttttctcc
     3361 ggaagaagaa agggtctttc aacgactgga agatgcaaga gactttgttt ctaaaatgga
     3421 atctatcata ggataaattg gctgaaggga agaatatgga taataaattt gcaaatggat
     3481 cagaatggag aaagtgggat ttacatatac acactcccgt atctttatgc tctgaatatg
     3541 gaggacagac agacgacgtt tgggaaaaat ttatttcaga aattgaacgt ttatctgaaa
     3601 gtattaaagt tatcggtatt aacgattata ttttcataga tggctataaa aaagtattag
     3661 attataaaaa caaaggtagg ttaaaaaata ttgatttaat tctccctgtt gttgaattgc
     3721 gactaaaaga gtttgtagga aatgcagatc ttaatagaat taattatcat ataatatttg
     3781 ctgatgagtc tattttagcc attgataata ttgaagcaca atttttatca gggttaaaag
     3841 gcaaagcaaa tttagattca gatagttcca gtggttatac ttggggagga gtggt
//
LOCUS       DAOQGJ010000106         3894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12953_length_3894_cov_3.960667, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000106 DAOQGJ010000000
VERSION     DAOQGJ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12953_length_3894_cov_3.960667"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PK683_02605"
     CDS             complement(<1..331)
                     /locus_tag="PK683_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07366.1"
                     /translation="MAEKKQKRYFNTSGPNHVWEHYTLLRPDLIEKGKDLVYRSRYFT
                     IWAPRQTGKSTYFRLLAAELEKEGYKVCHINFENYKNEPLPVFMDKFTQELSSFWEED
                     LKGHTIGE"
     gene            complement(612..2171)
                     /locus_tag="PK683_02610"
     CDS             complement(612..2171)
                     /locus_tag="PK683_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HNH07367.1"
                     /translation="MHYTIPPLTRWDMEEILMLIDQGKYFVLHAPRQTGKTSCILSLM
                     EYLNNEGRYNSLYINVEAAQAARENVHRGIQAIISVFATQHQMHFRTDLIEERWKEIT
                     EKAGAENALNKILHFWTENSSKPTVLFIDEIDALIGDTLISVLRQIRSGYADRPASFP
                     QSIVLCGVRDVRDYRIHSSQSKEIITGGSAFNIKAESLRLGNFSREEIRTLYLQHTER
                     TGQIFEEEIFELAWKYTEGQPWLVNALAYEACFRNKEAKDRTKQITEEMFIQAKESLI
                     LRRDTHLDQLTDKLKEDRVRKIIEPILKGDDSIEHFNDNDLLYLIDLGLIKRLTGGKT
                     EIANEIYREIIPRELVWTSQGTIHHEQIWYTDKITNRLDFHKLLTAFQEFFQENSESW
                     IDRFAYREAGPQLLLQAFLQRIINGGGIINREYGLGTKRTDLYIKWYPNKNDYSVSQR
                     TVIECKILHKSLEKTLQEGLSQTSEYADKCSAEESHLIIFDRTKDRPWKEKLFIRKMN
                     FQNRTITVWGM"
     gene            2423..3745
                     /locus_tag="PK683_02615"
     CDS             2423..3745
                     /locus_tag="PK683_02615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNH07368.1"
                     /translation="MMERIKIDIEDAVRADIENNPNMKKMKKYVDEMYSQNIYDAYLK
                     KFLKTALEVTAHSHHKLFSYIEDIMKELKIKANVRVFKAKESGTENALVMAEGENILI
                     VFCDNILNLIEPGPELKSIIGHELGHYVFKHHEKREEKYLQAIAAVFPAEALSAAEKK
                     FLKDPKNEELISLAFIASQINELNADRVGLLACKDLDAAVISGIKLAAGRVDKFGGYN
                     AQDYIAQAESLIKEGNYFDAEDIRITHPLEVLRAYALNYFYHSDVFKELTGKGSGKNR
                     LSDFNKILHKIIYINPLSEEADNRKSRTESSSAGKQSHMDPYEFELFRYIAVFNVVAA
                     DGRVTKAENEILCNFIKDDEIYEKIVSFSEKATQAEYIKAYENLAQKACGLDGRVKTS
                     IIKLMIKAAKADRKITDAEIGAIIGVAQSMDADRQCRVQLKNTFGLEY"
BASE COUNT         1134 a          769 c          844 g         1147 t
ORIGIN      
        1 cttcacctat cgtatgccct ttcagatcct cttcccaaaa ggaactcaat tcctgtgtaa
       61 atttatccat aaaaacagga agaggttcat ttttgtaatt ctcaaagttg atgtgacaga
      121 ccttgtatcc ttctttttcc agctcagcgg caaggagacg aaagtaagta cttttgcctg
      181 tctgacgggg agcccagatg gtgaagtacc ggcttctgta aacaagatcc ttcccctttt
      241 caatcaggtc aggacgcaga agagtataat gctcccagac atggttgggc cctgatgtat
      301 tgaaataccg tttctgcttc ttctcagcca tatgactgca atgtaaatga aaagctgtat
      361 gctgtaaagg acaaaagata ggcatctgct ttgctgaaga tacgtaacta ctcagcgaaa
      421 aacagattgt aagaaaattt atagagaaaa gcttgaaaag cggggtctta ggggctgcaa
      481 gcccctcgcc aaactgggat gcggctaatc ctcccgatag ggaccaacct tttcttgctt
      541 ttttaaagca atttcttgaa aaacggatat ttgtaactat gaagccggtt ggctgagtag
      601 ttacgaagat actacatacc ccagacggta atcgttctgt tctgaaaatt cattttcctg
      661 ataaaaagtt tttccttcca gggtctgtct tttgttctgt cgaaaatgat gagatgagat
      721 tcttctgcac tgcatttgtc ggcatactcg gaagtctgtg acaggccttc ctgaagagtt
      781 ttctccagag acttgtgaag gatcttgcat tcaattacag tcctttggga tacgctataa
      841 tcatttttat tcggatacca tttaatgtaa agatccgttc ttttggtccc cagtccgtat
      901 tctctgttga tgattccgcc tccattaatg attcgctgca ggaacgcctg gagaagaagc
      961 tggggtcccg cctctctgta ggcaaatctg tcgatccagc tctctgaatt ctcctgaaag
     1021 aattcctgaa atgcggtaag cagtttatga aaatccagtc tgtttgtaat cttatctgta
     1081 taccagattt gctcatggtg aatggtgcct tggcttgtcc atactaattc tctgggaatg
     1141 atctctctgt agatttcatt tgcgatttct gtcttgccgc cagtcaatct cttgataaga
     1201 cccagatcaa taagatagag caagtcattg tcgttgaaat gctcaatgga atcgtctccc
     1261 ttcaggatgg gctcaatgat ttttctcact ctgtcttctt tcaatttgtc tgtcagctga
     1321 tcgagatgag tgtctcttct caaaatcaga ctttcctttg cctgtatgaa catttcttct
     1381 gtgatctgtt ttgttctgtc ttttgcttcc ttattcctga agcaggcttc atatgcaagt
     1441 gcattgacaa gccaaggctg accttcagta tatttccagg ccagctcaaa gatttcctct
     1501 tcaaaaatct ggccggttct ctcagtgtgc tgcaggtaca atgttctgat ttcttctctg
     1561 gaaaagtttc ccagtcggag agactccgcc ttgatattga atgcagaacc tcctgtgatg
     1621 atttctttgg actgagagga atgaattcga tagtctcgaa catctcttac accgcataaa
     1681 acaatggatt gcggaaagga agccggacgg tctgcataac cggatctgat ctgacgcaga
     1741 acagaaatca aagtgtctcc gatcagagca tctatttcat ctatgaaaag aacagtaggc
     1801 ttgctgctgt tttctgtcca gaaatgaagg attttattca gagcattttc tgctcccgct
     1861 ttttctgtaa tttctttcca gcgttcttct atcagatctg ttctgaaatg catttggtgc
     1921 tgagttgcaa atacactgat gatcgcctga atccctctgt gtacattttc cctggccgcc
     1981 tgtgctgcct cgacattgat atacagagaa ttgtatctgc cttcattgtt cagatattcc
     2041 atcagagaaa gaatgcagct ggtttttccg gtctgcctgg gagcgtggag tacgaaatat
     2101 ttcccctgat ctatgagcat cagtatttct tccatatccc atctggtcaa tggggggatt
     2161 gtataatgca tttcagatct attcggcccg gcagtgttga aaaacttcat acaaaaccag
     2221 taaacctgaa gaaagtttct ttgtaaagaa aagattccac agccaaaaac acagtgttct
     2281 gtctgctcag aaattgtctt taaattcaga aggattgccc agtaatggtt caggactggg
     2341 ttgtgtaaac gtggaatgct ttgatgtctc cgttcagctc ctgcagagag tctggctggt
     2401 ctaaaaaaat tcgagggaat atatgatgga aagaatcaaa atagacatag aggatgctgt
     2461 cagggcagac atagagaata atccaaatat gaaaaaaatg aaaaaatatg tcgatgaaat
     2521 gtattctcag aacatctatg atgcatacct gaagaaattt ctgaaaacag cactggaagt
     2581 cactgcacat tctcatcata agcttttttc ctatatagaa gatataatga aagagctgaa
     2641 aattaaggca aatgtccgag tgttcaaggc aaaggagtcc ggtacggaga atgctcttgt
     2701 catggcggaa ggcgaaaata tcctcatagt gttctgcgac aatattctga atcttatcga
     2761 acctggtcct gagctgaaga gcattattgg gcatgaactg ggacattatg tatttaaaca
     2821 tcatgaaaaa agagaggaaa aatatcttca ggcaattgct gctgtttttc ctgctgaagc
     2881 attgagtgct gctgaaaaga aatttctgaa agatccgaaa aatgaagaac tgatcagtct
     2941 tgcattcatt gccagtcaga taaatgaact gaatgcagac agggtgggac tgcttgcctg
     3001 caaggatctg gacgctgccg tcatatcagg aatcaagctt gccgcaggca gggtggataa
     3061 attcggtggg tataatgcac aggattatat agctcaggca gagtcactga ttaaggaagg
     3121 aaactatttt gatgcggagg acattcggat tacacatcct ttggaagttc ttcgtgcata
     3181 cgcactcaat tatttctatc actcggatgt attcaaagaa ctcactggaa aaggaagcgg
     3241 aaagaaccga ctttcagatt tcaataagat actgcataaa ataatataca ttaacccgct
     3301 gagtgaagaa gcggataata gaaaatccag aactgagagc agttcagcgg ggaaacagtc
     3361 tcatatggat ccttacgaat ttgaactttt ccgttatatt gctgtgttta atgttgttgc
     3421 tgcagacggg agagtgacta aggcagaaaa tgagattctc tgcaatttta tcaaggatga
     3481 tgaaatttac gaaaagattg tctctttctc ggaaaaagca actcaggcag aatatataaa
     3541 agcatatgaa aatctggcgc aaaaagcctg cggactcgac ggccgagtga aaacttccat
     3601 tatcaaactg atgatcaagg ctgcaaaagc ggacaggaaa atcactgatg cagaaatcgg
     3661 tgccatcata ggagtggctc agtccatgga tgcggacaga cagtgcaggg ttcagctgaa
     3721 gaataccttt ggtctggagt attgagaatg ctgattgtcg gcagaccgat cggaatattc
     3781 tgatataaaa atactgtaaa aatcattaaa aaaattaata gaaagattaa aatactgatt
     3841 atcagaaaaa acttcatttt tatctctttt gtattgatcc ggaatttcag tctc
//
LOCUS       DAOQGJ010000107         3890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_12973_length_3890_cov_2.813038, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000107 DAOQGJ010000000
VERSION     DAOQGJ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12973_length_3890_cov_2.813038"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..345
                     /locus_tag="PK683_02620"
     CDS             148..345
                     /locus_tag="PK683_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07369.1"
                     /translation="MEFLNLEKELKDAEGKTADLHKKMLGQISRRSKLNWELYSASRS
                     DAEYEKLMKREKAFLYPGKQP"
     gene            complement(383..2179)
                     /locus_tag="PK683_02625"
     CDS             complement(383..2179)
                     /locus_tag="PK683_02625"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH07370.1"
                     /translation="MEKCSYLECTAITFFENLFFYFLFTLPFFFSFWVIWKSKWQLRR
                     IQSVQRATSHHIRFDLFYSFVSFFIIAFTAGYITYLAVEGKTLMYFDASKYGWGWIIV
                     SFFIILFLDDMFFYWAHRAIHHPKLYALIHKVHHESTDPSPFTSFSFHPIEALIEIAT
                     ALILPLILPMHFGVFTAWQMFSMLNNVLGHLGYELYPKGWTKFPVLRFKTASVHHNMH
                     HQLFNGNYALYFTWWDRWMKTEFKDYESRHQEIFERENTRKNSEGFYILKVSQIQQEQ
                     NDAFTVFFDEVPGVFREFSAGQHITLKAVIEGKSYYRTFSLSSVPNAGRSASVTIKKI
                     PGGKVTNYLAENLKAGDELEVSAPSGTFALTPEPANQKHYVMLAGGSGITPLHSMIGA
                     ILLFEPKSRITLLYSNRNSSSVILVKGIDNLVIKYPNQISVHYFFTENSGERIGKDAI
                     LRIFGQTDLNTLFFYLCGPQTMIQDMEQILTALGITDERIFKEYFTVSGGNDSSSENS
                     ASASVQAEIYGKKYSFKCGTGKTLLQSAMDNNVPIPYSCQRGVCGTCRLKCTKGNVTL
                     KNNRYLSDEKLKEGFILTCQAVPVSDEIELHS"
     gene            2451..>3890
                     /locus_tag="PK683_02630"
     CDS             2451..>3890
                     /locus_tag="PK683_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07371.1"
                     /translation="MKKRYYLSMLSLFPIIMLFDSIYFIYHRSLNIFLGLGFVHFMLF
                     VVLNFFGSFFIYRPIDRLGLPEGNREKAEKRIQMLTKISAGWILFIGSLYVAIAVLPL
                     YFFPDIFQSPEDFTIEKIPERFFFFSILPSIFFVYAVFPSFLSYFIVNDFSLDLKDYA
                     ASEFNIRYSAEKKRIGFTLFIVFLFLVILPSLLVILELKMAFELSDKYSQFTKLSPLE
                     TLLIDRFVVFIGTVIAVVLITRSFTKPIDVLLRTISRVNGGDYSAKAAVITEDEIGIL
                     TKEFNEMVNGLRERELIRDTFGKYVTKDVASHILGQEIHLEGEVRDCTILVTDIAGYT
                     AISEELSPKEIVQMLNEYFSVLVGIIQKHKGVVNKFIGDSIFAMFNVPLDDEHHAANA
                     IRAALEIQEMAAKARFGKNRRLSTRIGINSGVVVAGNIGSSDRMEYTVIGDDVNIASR
                     LEQLNKEYGTDILVGENTYRLAAEQFQFTQ"
BASE COUNT         1164 a          758 c          835 g         1133 t
ORIGIN      
        1 ggaaaaatat gataaattga tatttagtga tgaggctata catacgctag taaagaaatt
       61 agagacaaaa gttttggaaa gaaaaatatg ggcgtttttg aatataaaaa tgttaaagat
      121 attcttttca gaaacaggac agaggtcttg gaattcctga acctggaaaa ggaactgaag
      181 gatgctgagg ggaagactgc agacctgcat aaaaaaatgc tgggacagat aagccgaagg
      241 tcgaaactga actgggagct gtattccgca agccgttcag atgcagaata tgaaaaactg
      301 atgaaaagag aaaaagcttt tctttatccg ggaaaacagc cttgaagaaa gtgaatttcc
      361 aaggcattaa attttcatta tgtcaggaat ggagttcaat ttcgtcagag acaggaacgg
      421 cctgacaggt cagtataaag ccttctttca gtttttcgtc agagagatac cggttatttt
      481 tcagagtgac attcccttta gtgcatttca gccgacaagt gccgcagact cctctctggc
      541 aggaatatgg aatcgggaca ttgttatcca tcgcactctg aagcagcgtc tttcctgtcc
      601 cgcacttgaa ggaatatttt tttccgtaaa tctctgcctg tactgaagcc gaagcggaat
      661 tttccgaact ggaatcattt cctccgctga cagtaaaata ctccttaaaa attctctcat
      721 cagtaattcc gagtgcagtc aggatctgtt ccatatcctg aatcatagtc tgcggaccgc
      781 atagatagaa aaagagcgta ttcaggtcag tctgaccgaa gattctgaga attgcatctt
      841 tgccaatcct ctctccggaa ttttccgtaa agaaataatg gacagaaatc tgattgggat
      901 atttaataac taaattatct atacctttta ccagaatgac gctggaggaa tttcgattgg
      961 aatagagcag agttattctg cttttgggtt cgaaaagcag aattgctccg atcatactgt
     1021 gcaagggagt gatcccgctt cctccggcga gcatcacata gtgtttctga ttcgccggct
     1081 ccggtgtgag agcaaatgtt ccgctcggag cagaaacttc cagttcatct cctgccttta
     1141 ggttctctgc aagataattc gttacctttc ctcccggaat tttttttatt gtgactgaag
     1201 cagacctgcc ggcattcgga acggaggaaa gtgaaaatgt tctgtaatag gattttcctt
     1261 ctatcacagc cttgagagta atatgctgcc ctgcgctgaa ctctctgaat actcccggta
     1321 cctcatcaaa aaaaacagtg aatgcatcat tctgttcctg ctgtatctgc gaaactttga
     1381 ggatataaaa accttcagag tttttccttg tattttcccg ttcaaaaatt tcctgatgcc
     1441 gtgattcata atccttgaac tctgtcttca tccatctgtc ccaccaggta aagtacagag
     1501 cgtagttccc gttgaaaagc tgatgatgca tattatgatg gactgaagct gtcttgaacc
     1561 tgaggactgg aaattttgtc catcctttcg ggtacagttc atagcccaaa tgaccaagga
     1621 cattgttcag catcgaaaac atctgccagg cagtgaatac accgaaatgc atcggaagaa
     1681 tcagcggcag aatcaaagca gttgcgattt caatcagcgc ctcaatcgga tgaaacgaaa
     1741 aggaagtgaa aggagaagga tcagtgcttt cgtggtggac tttgtgaatc agagcataga
     1801 gtttcggatg atgaattgca cggtgtgccc agtaaaaaaa catatcatca agaaacagga
     1861 tgataaagaa acttacaatg atccagcccc agccgtattt ggatgcatca aagtacatca
     1921 gtgtttttcc ctcaaccgcc agataagtga tataaccggc tgtgaaagct atgataaaaa
     1981 aggaaacaaa ggaataaaag agatcaaacc ggatatgatg ggatgtggct ctttggacag
     2041 actgaattct tctcagctgc catttgcttt tccatatcac ccagaaggaa aaaaagaacg
     2101 gaagagtaaa aagaaaataa aaaaacagat tctcaaaaaa agtgatggca gtacattcta
     2161 aataactgca tttttccatt cagtatctcc gaaatttttt tcgctgagca gttactattt
     2221 ttccagcggc agtattttgt caagcatcgg atatttgagg aaaagcagga tgtcgtaatc
     2281 tttctgttcg gactgcagac tgatttttac ggtgcagagc agtcccggta cgcatacatc
     2341 cgcttaggaa tcatgaattt tttactggaa tccaacgatc agcataactt cttgactgta
     2401 tttccgttca atatgcagtg ttatcagaca tcccaaatac tcaggacttt atgaaaaaaa
     2461 gatattatct ttccatgctc agtctttttc cgattattat gctttttgac agtatatatt
     2521 ttatatatca tcgttcactg aatatattcc tcggactggg atttgtgcat tttatgcttt
     2581 ttgttgtttt gaactttttc ggttccttct ttatttacag accgattgac agacttgggc
     2641 tgcctgaagg aaacagggaa aaggcagaaa agagaattca gatgctcacg aaaatatctg
     2701 ccggatggat acttttcata ggatctcttt atgtggcaat tgcagtactg cctctgtatt
     2761 tttttccaga catatttcag agtcctgaag attttacgat tgaaaagatt cctgaaagat
     2821 ttttcttttt ctcaatcctt ccatctatat tttttgtata cgctgtattt ccttcttttc
     2881 tttcttattt cattgtgaat gatttcagtc tggatctgaa agactatgct gcttctgaat
     2941 tcaatatccg gtattccgct gaaaaaaaac ggatcggatt tactctgttc atcgtttttc
     3001 tttttctagt gattcttcct tctcttctag tcatcctgga actgaaaatg gctttcgaac
     3061 tgtcggataa gtattcccag tttacaaaac tcagtcctct cgaaacactt ctgatcgacc
     3121 gctttgtagt attcatcgga acagtgattg cagttgtgct gatcacacgt tcttttacaa
     3181 aaccgattga tgttcttctc agaacaatca gcagggtaaa cggtggagat tattctgcga
     3241 aggctgcggt gattacggaa gatgagatcg gcatactgac aaaagagttc aatgagatgg
     3301 tgaacggtct gcgggagcgc gaactgatac gggatacttt cggaaagtat gtgaccaagg
     3361 atgtggcttc tcatatactc ggtcaggaaa ttcatctgga aggagaggtc cgggactgca
     3421 caattctcgt cactgacatt gcaggatata cagccatctc tgaagaactc agtccgaaag
     3481 aaattgtcca gatgctgaat gaatacttct ctgttcttgt aggaatcata cagaaacata
     3541 aaggagttgt taataaattt attggtgatt ccatttttgc tatgttcaat gttcccttag
     3601 atgatgaaca tcatgcggca aatgcaattc gagcagcgct ggaaatccag gagatggcag
     3661 caaaagcaag atttggaaaa aacagaagac tgagtacaag aataggaatc aattcagggg
     3721 ttgtagtggc aggaaacata ggttcctcgg acagaatgga atacacagtg atcggagatg
     3781 atgtcaatat tgcctcacgg ctagagcagc tgaacaagga atacggcacg gacatacttg
     3841 tcggggagaa tacataccgc cttgccgcag aacagtttca gttcacacag
//
LOCUS       DAOQGJ010000108         3882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13028_length_3882_cov_3.422785, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000108 DAOQGJ010000000
VERSION     DAOQGJ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13028_length_3882_cov_3.422785"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK683_02635"
     CDS             <1..526
                     /locus_tag="PK683_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07372.1"
                     /translation="KEGELSDDYTLLKIHYTAKYFSNITQEYQKLFQQAEQLSSDQKE
                     KIKELYKKALKYNESLEVLKKLSEIYYEEKSWSEFIHFAERLVKIRPSESLYYYRLSY
                     AFLQIEDYHQAADYGESLKLREPENVENLMNLIEIYRRLNRIERIQELIHKGLYYQPD
                     NTKLLEQKALVAGF"
     gene            735..1313
                     /locus_tag="PK683_02640"
     CDS             735..1313
                     /locus_tag="PK683_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNH07373.1"
                     /translation="MNGELNYDEVWAMFQETDRRFKETAEQFKETDRQFKETDKQFKA
                     TDRKLLKLERMFTGQWGKLIESLVEGDLVNLLNARGIPVNQTGQRIKKRYNKKDYEID
                     LIARNGKEVVFVEVKTTLKPDDVLNFTEKLKMAKTVFPEYSENILYGTVAYLRSESNA
                     EQFAMKNGLLVIKATGKSASIINDKEFQPAQF"
     gene            complement(1316..3106)
                     /locus_tag="PK683_02645"
     CDS             complement(1316..3106)
                     /locus_tag="PK683_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNH07374.1"
                     /translation="MKKAVQIFQKFRKTRWFDFACILWYGIIASLAFVPFHLTHLIWL
                     APFPLFWMEKKYRKSLKKLIIVGFMIGMSATVLSYNWMIHLLQVFGGFPYPIAFFLFL
                     FYALVTNSRYAIYLVIYSRLKMRIKGYGAFIAGFSILCAEFFSYQLFPYYLGNILAWN
                     NVLAQNAEITGVYGLSFLVAVVSYALFRSASGILHGKKILKKKPFQRMLLFPSSVLVL
                     FVLIGTYLYFKWNSYVPEKTKQILMIQPNAPLEFRDGRSVMESLTEIMQNVEDLAVKA
                     ADEKKPDIIVMPESGVPFFSTHNTPANVLYNPSYYYRYEALIYLLANRYKTNVYFNEI
                     DAIFLNDKASPKNQRFYNNSVVFSPNGERGEHYRKSYLLAFGEYFPGGEWFPIIYDII
                     PQVGRFLEGKEQNLVTYYKNTKEPEVLKKSHLKWMDSSYMNMNAIRSYYADYKSETAE
                     EGKFLPLICYEVILPEFVRKFYSAGNPDFIINITNDKWYGLSIETFQHLDLARIRSIE
                     ARKWMVRSTNSGTSVFVDHMGRILNENYTAQEASATYAARVPVMREPPTFYVRFGNLF
                     AWLYMVFFSGYWIFRLRGAFFMKKTDGISA"
     gene            complement(3109..>3882)
                     /locus_tag="PK683_02650"
     CDS             complement(3109..>3882)
                     /locus_tag="PK683_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNH07375.1"
                     /translation="TEKNFKPFDRKRTPDVRYAGMMQYDGDLKLPEKYLVSPPAIDRT
                     LGEYMASEKITQYAISETQKFGHVTFFWNGNRSGYIDKSTETYVEIPSDVIPFDQKPD
                     MKAPEITDTLLTALDGRNKFLRVNFANGDMVGHTGSFDATVRAMETLDKCMKRLKDAA
                     DRLGAVMLVTADHGNADEMYQLEKKGGVQMQNGKPVPKTSHTLNPVNLCLYDPKHRFS
                     LKTNSASAGLGNIASTVLDILGYEAPEDYLESLLVRKGS"
BASE COUNT         1181 a          796 c          810 g         1095 t
ORIGIN      
        1 gaaggaagga gaactgagtg acgattacac tctactgaag attcactata ctgcaaaata
       61 tttttccaat attacccagg aataccagaa actgtttcag caggcagagc agctttcatc
      121 agatcagaag gaaaaaatca aagagcttta taaaaaagca ttaaagtata atgaaagtct
      181 cgaagtattg aaaaagctgt ctgaaattta ctatgaagaa aagagctggt ccgagtttat
      241 tcattttgcc gagcgtcttg tgaaaatcag accgtccgag tctctttatt attacaggct
      301 ttcctatgca tttctgcaga tcgaagacta tcatcaggcc gccgactacg gtgagagtct
      361 gaaactcaga gagccggaaa atgtggaaaa cctgatgaat ctgattgaaa tttacaggag
      421 actgaaccgg atagaaagga ttcaggagct gattcataaa ggactttatt atcagcctga
      481 caatacaaag cttctggaac agaaggcgct tgtggcaggg ttttgactga atcgggtcgg
      541 gaggtgcttg tgcactgccc cgtctcaaag gcattctcct ggaaacctgt ttcagtgaat
      601 gccgaagctc aggaaaattg catccttgaa ctgaaaactc gcaatatgtc atataatttt
      661 aaaagtcata ttaatgattt tttgttgact ttttccctgt cctgtctgac tattgagtca
      721 ggaggaatca ttcaatgaac ggtgaattga actatgatga ggtctgggca atgtttcagg
      781 agacagacag acgttttaag gagacagctg agcagtttaa ggaaacggat agacagttta
      841 aagagacgga caaacagttt aaggcaacag acagaaaact gctgaaactc gaaaggatgt
      901 ttactggaca gtgggggaaa ctgattgagt cgctggtgga aggtgatctt gtaaacctgc
      961 tgaatgcacg aggaatccct gtaaatcaga ccggtcaaag aattaaaaaa cgctataata
     1021 aaaaagatta tgaaatagat ctcattgcca gaaacggaaa agaagtggta tttgtggaag
     1081 tcaaaacgac tttaaaaccg gatgatgttc tgaacttcac agagaaactg aaaatggcaa
     1141 aaacagtttt cccggagtac tcagaaaata ttctgtacgg aacagtcgcc tatctgagat
     1201 cggaatccaa tgcagaacag tttgcgatga aaaacggtct gcttgtcatc aaagcgactg
     1261 gaaaaagtgc aagcatcatc aatgataagg aatttcagcc tgcacagttt tgagatcagg
     1321 cagaaatgcc gtctgttttt ttcatgaaaa atgcacctct gagtctgaat atccagtatc
     1381 ccgaaaaaaa taccatatag agccaggcaa aaaggtttcc aaaccgcaca taaaacgtgg
     1441 gaggttctct cattacagga acgcgtgctg catacgttgc acttgcttcc tgagctgtat
     1501 agttctcatt taatatacgt cccatatgat ctacgaatac ggatgtacct gaattagtcg
     1561 aacgcaccat ccattttctg gcttcaatgg aacggattct agccagatcc agatgctgaa
     1621 atgtttcaat gctcagtccg taccatttgt cattcgtgat attgatgatg aaatcaggat
     1681 ttcctgccga ataaaatttt ctgacaaact caggaagaat cacttcgtag cagatcagag
     1741 gaagaaattt tccctcctct gcagtctctg acttatagtc cgcataataa cttcgaatgg
     1801 cattcatatt catataactc gaatccatcc atttcagatg cgacttcttc agaacctcag
     1861 gctcttttgt gtttttgtag tatgtaacga gattctgttc cttgccttcc agaaaccttc
     1921 ccacctgagg aatgatgtca tagatgatcg gaaaccattc tcctcccgga aagtattccc
     1981 cgaaagcaag cagataggat ttcctatagt gttctcccct ttctccgttc ggtgaaaata
     2041 cgaccgaatt gttgtagaat ctctgatttt tgggagatgc tttatcattt aaaaatattg
     2101 catcaatctc gttaaaatac acgtttgtct tgtatctgtt tgccagaaga tagatcagtg
     2161 cttcgtacct gtaatagtaa gaaggattat aaagaacatt agcaggtgtg ttgtgagtgg
     2221 aaaagaaagg aactcctgat tcaggcatga cgatgatatc aggttttttt tcatctgcgg
     2281 ccttcactgc cagatcttcc acattctgca taatttccgt cagagattcc attacagatc
     2341 ttccatccct gaactccaga ggagcattcg gctggatcat cagtatctgt tttgtcttct
     2401 caggaacata ggaattccat ttgaaataca gataggttcc gattagaaca aagagcacaa
     2461 gaactgagga tgggaaaaga agcattctct gaaaaggctt tttcttcaga atctttttac
     2521 cgtgaaggat tcccgaagct gatctgaaaa gagcatagga aacaacagcc acaagaaaac
     2581 tcagtccgta gacacctgta atttctgcat tctgtgccag aacattgttc caggccagta
     2641 tgtttcccag ataataagga aaaagctgat aactgaaaaa ctcagcacat agaatgctga
     2701 atcctgcaat aaaagcacca taacctttaa ttcgcatttt caggcggctg taaatcacca
     2761 ggtaaattgc atatctggaa tttgtcacaa gtgcataaaa cagaaacagg aaaaaagcaa
     2821 tcggataagg aaaccctccg aaaacctgca gcagatgaat catccagtta taggaaagga
     2881 ctgtcgcact cattccgatc ataaaaccga cgataatcag tttttttaat gattttctgt
     2941 attttttctc catccagaaa agagggaaag gtgcgagcca gatgagatga gtcagatgga
     3001 aaggaacaaa tgcgagactt gcgataattc cgtaccatag aatgcaggca aaatcaaacc
     3061 atctggtttt tctgaatttc tgaaatatct gaactgcttt tttcaagatc agcttccttt
     3121 tctgacgaga agactttcca gatagtcctc aggtgcttca tatcccaaaa tatccaaaac
     3181 ggtagacgct atattcccca gtcccgcact tgctgaattt gtcttcagtg aaaacctgtg
     3241 cttcggatcg taaagacaga gattgaccgg attcagtgta tgacttgtct ttggaacagg
     3301 cttcccgttc tgcatctgca ccccgccttt tttttcgagc tgatacatct cgtctgcatt
     3361 tccgtgatct gcagtgacga gcatcactgc tccgagtctg tcggcggcat ccttgagtct
     3421 tttcatgcat ttgtccagtg tttccattgc tctgactgtt gcatcaaaac tgccggtatg
     3481 accgaccata tctccgtttg caaaattgac tctcaggaat ttgtttcttc cgtccagtgc
     3541 tgtcagaagc gtatccgtaa tttcaggagc cttcatatcc ggtttctgat caaatggaat
     3601 gacatcggaa ggaatctcca cataggtctc cgtacttttg tcaatataac cgcttctgtt
     3661 tccgttccag aaaaaagtca catgtccgaa tttctgagtt tctgaaatag catactgtgt
     3721 aattttttca gatgccatat attctccgag tgtcctgtca attgcagggg gagatacaag
     3781 atatttttca ggaagtttca gatcgccgtc atactgcatc attcccgcat atctgacgtc
     3841 gggcgttctc tttctgtcaa aaggcttgaa gttcttttca gt
//
LOCUS       DAOQGJ010000109         3880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13038_length_3880_cov_3.020654, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000109 DAOQGJ010000000
VERSION     DAOQGJ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13038_length_3880_cov_3.020654"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /gene="murB"
                     /locus_tag="PK683_02655"
     CDS             <1..892
                     /gene="murB"
                     /locus_tag="PK683_02655"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNH07376.1"
                     /translation="PCFEDVSLSSYCSFRIGGNSPMLCEVQNNDHIFIFISATERFEI
                     PWKILGGGTNILISDHPDDFVILQLGGVFKEYSEIQEGLFRIGAAALTTPVFRKISQS
                     GYTGLEFLSTIPGSVGGAVIQNAGCYGGELFDFTLDVEFIREGRLYKEKRENIQYGYR
                     NTQFLKNKDSLITSVTMTAQKGDIILIEESLKEKRDKRNSSQPPNKKSAGSVFKNPNI
                     PDEKGNTLKAWQLIDRAGLRGTSKGGARIAEEHCNFIVNTGGATAADVHYLIQLILEK
                     VSRTSGILLEREIEFFGQIP"
     gene            complement(895..1422)
                     /locus_tag="PK683_02660"
     CDS             complement(895..1422)
                     /locus_tag="PK683_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07377.1"
                     /translation="MTAKNSAVPFAQISTDTAVFTVRSESITYEDWKGPAFLPIIPSR
                     TSGLEAEHLWLGFILKTKSDPEKFKGKINIKDFTLKLDNGTEAVPETVYLFEEKETLE
                     TAVTDFQNYPFKDGTTFSLKYPEVIYSKVKWYEFSAVLQFKDRKEKSPVFHMTPAKQR
                     TFDLFDFPPIIPMGR"
     gene            1825..>3880
                     /locus_tag="PK683_02665"
     CDS             1825..>3880
                     /locus_tag="PK683_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07378.1"
                     /translation="MNKPLKIRNLKRPDSSCDFPKKHTILLVDDEAGNLEAVKRLLET
                     EYNVITALSGYEALQILSDPEFCLCIHLIISDQRMPEMKGVEFLKKSIKLVPNAVRIL
                     LTGYTDVNEIIDSVNQGHIFQFIAKPVETQSFLVSVRRAAEAYDLENRNVQLIKELEN
                     KSTSLAESLEKLNELNLSLEKKVAERTEELETLNTFSRKINSDTDLTEIFIHISQYIY
                     GRYGIETLSLYLPEESKNYLYTYKMYSYIKIPDKAYSQFSSIRVPLSERKNILTSVFH
                     RKKPFYLRRLRNNMFSDEYSSGNIPDPETASFLLVPLVVREQTVGIFNFSKVSQRLNL
                     EKREIVYLFSLCSQAAGIIHTANLLHQTEKARLDLERQKEALEESRRKEEEARKELET
                     INDFTRVINETLNLEEVIDSMFRYIEKKFGLESSFICMHNPSNNRLLLQNYARLPHFS
                     EEQTGIIEAFKVKLDDKKGINYHVFSSKKSFYAPSLHKTSERHYYKAEKELFDALKLE
                     FALIIPLLIKNSVIAQVIFTSFREKVKLSKVQIQKITFFCDQVAGAIYRNRLMKETEE
                     AKKEAYIEGAIAQIAQEEAVKEKQKTEKMNKELSVLNEFAKTINAKNDLDYVLQTVLD
                     YMHESYGFQQVWLQTVDRENRLLVPYRLFSKDVDLPRPSKAWEKFFHSEKWQLNSQSG
                     ASY"
BASE COUNT         1236 a          703 c          837 g         1104 t
ORIGIN      
        1 tccctgcttc gaggatgttt cactttcctc atactgttct ttccgaattg gaggaaattc
       61 ccctatgctc tgcgaagtac agaataatga tcatatattt atttttatct cagccacaga
      121 acgtttcgag attccctgga aaatcctggg cggagggaca aatattctga tatcagatca
      181 tcctgatgat tttgtcattc ttcagcttgg gggtgtgttt aaggagtatt ctgaaatcca
      241 ggaaggactg ttcaggatcg gtgcggctgc actcacgact cctgtgttca ggaaaatatc
      301 ccagtccggt tatactggtc tggaatttct gagcacaata cccggatcgg taggaggcgc
      361 tgtgattcag aatgcagggt gctacggagg agagcttttt gattttactc tggatgtgga
      421 atttatcagg gagggcaggc tgtacaagga aaagagggag aatattcagt atgggtacag
      481 aaatactcaa tttttaaaaa ataaggactc tctgatcaca tctgtcacaa tgactgccca
      541 aaagggagat attattctga ttgaagaatc cttaaaagaa aagcgtgata aaagaaattc
      601 ttctcagcct ccgaacaaaa aaagtgcagg atctgtattc aaaaatccta atattccgga
      661 tgagaaagga aatactctga aagcctggca gctgattgac agggctggac tgcgaggcac
      721 ttcaaaaggc ggtgcacgga ttgcagagga gcactgcaat tttattgtaa atacaggtgg
      781 tgcgacagct gcggatgttc attatctgat acagctgatt ttggaaaaag taagccggac
      841 ctccggaatc ctcctggaaa gagagattga attttttgga caaatccctt aaattcaccg
      901 ccccatcggt atgattggcg gaaagtcaaa aagatcgaat gtcctttgtt tggccggagt
      961 catatgaaac acaggacttt tttcctttct gtccttgaac tgaagaacag cactgaattc
     1021 ataccatttt actttggagt agatgacttc aggatatttc agtgaaaaag tggtgccgtc
     1081 cttaaaggga tagttctgaa aatcagtgac tgcagtttcc agtgtctctt tttcttcaaa
     1141 taaatacact gtttcgggaa cagcttcagt tccgttatcc agctttaatg taaaatcttt
     1201 tatattaatt ttacctttga atttttccgg atctgatttt gtcttcaata taaatccaag
     1261 ccaaagatgc tctgcttcca gccctgaagt cctggacggt ataataggaa ggaatgccgg
     1321 tcctttccag tcttcgtacg tgattgattc tgatctgacc gtaaatacgg cagtatcagt
     1381 ggatatctgt gcaaacggga ctgcactgtt tttcgcagtc atctcatact gtttctcagg
     1441 ttttgagtaa tacaggactt tgacagatga gcaaccatat agataaaata cagatgcgat
     1501 aatatatatt ttcagtattg ttttcatgag tataaaagtg tatagaaact cggtttccag
     1561 tcaaataaaa atactgtttt ccgggaaaaa taggggaaaa caattaattt tgcgtatgtc
     1621 aaaattcagg tttaaatatt ttatgatgaa cagataacag ccaattttta gaacatatcc
     1681 ttatttagtg gttgactcgc tgtctcacag agtgaatctg tgaatcagac gaatacagtg
     1741 acttgtcaat attgacagtg tcttctgtct gcagatgatt tgaggtcaga tttctcagca
     1801 gatgccgatt gatggaaaaa aataatgaat aaaccattaa aaatcaggaa tttgaaaaga
     1861 ccggattcaa gttgtgattt tcctaaaaaa catacgattc ttcttgtaga cgatgaagca
     1921 ggaaatcttg aagccgtaaa gagacttctg gaaacggagt acaatgtaat tactgctctc
     1981 agcggatatg aagctcttca gattctttcc gaccctgaat tctgtctctg catccatctg
     2041 attatttcag atcagagaat gcctgaaatg aaaggtgttg agttcctgaa aaagtcgata
     2101 aagcttgttc cgaatgcagt gcgtattctg cttaccggtt atactgatgt gaacgaaatc
     2161 attgattcag tgaaccaggg gcatatattt cagtttattg caaaacctgt tgaaacacag
     2221 agttttcttg tttcggtcag aagggcagct gaagcttacg atcttgaaaa caggaatgta
     2281 cagctgatca aggagctgga aaataaaagc acgagtcttg cggaaagcct ggaaaagctg
     2341 aatgaactga atctttcttt agagaagaaa gttgcagaaa gaactgaaga acttgaaaca
     2401 ctgaatacat tcagcagaaa aatcaattca gacacagacc tgacagaaat attcattcat
     2461 atttcacagt atatatacgg gagatacgga atagagaccc tttccctgta tcttccggag
     2521 gaaagcaaaa attatttata tacttataaa atgtattctt atattaaaat tcctgataaa
     2581 gcttacagtc agttcagcag cattcgagtt cctctttcag aaagaaagaa tatactgacg
     2641 tctgtctttc acagaaaaaa gcctttctat ctccgaagac tgagaaataa tatgttttct
     2701 gacgaatatt cttccggaaa tattccggat ccggagacgg catcatttct gttagtgcct
     2761 cttgttgtcc gcgagcagac agtgggaata ttcaattttt caaaagtgtc ccaaaggctg
     2821 aatcttgaga aaagagagat tgtatatctt ttcagtctgt gttcgcaggc tgccggaatt
     2881 attcatacag ccaatcttct tcatcagaca gaaaaagcaa gactggacct tgaaaggcag
     2941 aaagaggctc ttgaagaatc cagaagaaag gaggaagagg caagaaagga acttgagacc
     3001 atcaatgatt ttacaagggt catcaacgaa actctcaatc ttgaagaagt aatagactcg
     3061 atgttccgtt atatagaaaa aaaatttgga ctcgaatcaa gtttcatatg catgcacaat
     3121 ccttcaaaca acagactgct tctgcagaat tatgccaggc ttcctcattt ttccgaagag
     3181 cagacaggaa taattgaagc atttaaagta aagctggatg acaaaaaagg gataaattat
     3241 catgtttttt catccaaaaa atctttttat gcaccaagcc tgcataagac ttccgaaagg
     3301 cattattaca aggcggaaaa agagctgttt gatgctctga agcttgagtt tgcactgatt
     3361 attccgcttc tgataaagaa cagtgtcatt gctcaggtta tttttaccag tttcagagaa
     3421 aaagtaaagc tgagtaaagt tcagatacag aaaattactt ttttctgcga tcaggtcgcg
     3481 ggagctatat acagaaaccg tctgatgaaa gagacggaag aggccaagaa agaagcttat
     3541 atagaaggcg cgattgcaca gattgctcag gaagaggcag taaaggaaaa gcagaaaacc
     3601 gaaaagatga acaaggagct ttctgttctg aatgagttcg caaagacgat caatgcaaaa
     3661 aatgatctgg actatgtact gcagacagtt ttagattata tgcatgaatc atacgggttt
     3721 cagcaggtgt ggcttcagac tgtagacagg gaaaacaggc ttcttgttcc ctaccggctt
     3781 ttcagcaagg atgtcgatct tccgaggccg tccaaggctt gggaaaaatt tttccattca
     3841 gaaaaatggc agctgaattc tcagtccgga gcatcctact
//
LOCUS       DAOQGJ010000110         3859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13147_length_3859_cov_3.643796, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000110 DAOQGJ010000000
VERSION     DAOQGJ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13147_length_3859_cov_3.643796"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PK683_02670"
     CDS             complement(<1..808)
                     /locus_tag="PK683_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07379.1"
                     /translation="MKHTKYIILSFICISVCNFMFQSPNSRPRRPGNFPIIRVNVDQS
                     VFPPGITSSVWNSTVENSSLSWFQNSSAKVSYSVKALSTERSPLVPSEISCSDSAKNS
                     LSKEDTLYLSQGADPDCTGTSCSFLWSCGDEILHSDIQINSEYSNELSDPNILNTVLT
                     HEFGHSLGLDHCSPGDTVSQCQNRLNLGASNTDPNEFSVMNKFTDSMKNISTDDSDGL
                     KKLYGEMFNPFPRTGIYALNAEEIALFSTAENQEASISHLIGTPEQQVKTY"
     gene            complement(885..1643)
                     /locus_tag="PK683_02675"
     CDS             complement(885..1643)
                     /locus_tag="PK683_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HNH07380.1"
                     /translation="MNRLLTMTKLSAVNNYNEKISLNEKPTLFELLELRNGAVGLMGH
                     NMVPQGDYAFFELTLGTKQEIQIDGQFLPLTIEDAGQSVLRFMGPFALRGGRLTEVFL
                     DFDPNTSIFYIPERGYILDPTLLTTSVISMTPVQDGRIRDALGQRSNTVTSESDLIFQ
                     GTVKSVSPFVSQNIYGKNTVYSQNTLTVEDRLRGQVSTPEFSIQTPGGTVNSSTLKVK
                     GMPEFTAGESCLVFLKKYGNRYAPTRGEMGKISF"
     gene            2221..3087
                     /locus_tag="PK683_02680"
     CDS             2221..3087
                     /locus_tag="PK683_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07381.1"
                     /translation="MNNKQKAAQQYDSQSASFSKIIKPITLVITICIAVAFTELSAQM
                     PRFSKTVILKNGIVFEGVDAIRTEELFTITTPEGMTKVYQSKEISIITWDGSNDLKNA
                     PQKPSNRKWSEDQGYMNWKDAMKKCGSIGMRLPKPQELKEAYAAGITESWKSDARYYW
                     SSERSKEDNWIKFFNIYTGITGSNFETSYHKVRCLISDYIWSEDQGRMNWEDAKKKCE
                     SIGMRLPTTDELKEAYAAGITKSWKKISTDNYDIWSSDPGRDDTAIALDNDTGEALPN
                     DREGKNPVRCLH"
     gene            complement(3167..3742)
                     /gene="pnuC"
                     /locus_tag="PK683_02685"
     CDS             complement(3167..3742)
                     /gene="pnuC"
                     /locus_tag="PK683_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNH07382.1"
                     /translation="MEKIISITALVSGLICVVLAARGHIGNFIFGILNSAAYAYIAWL
                     NGLFGEVGLNLIFFIPVNIAGIFLWKDRILDGTVIMKKMKLMNILYILAGAFLLTFLF
                     GWSLSFIAGQNTPYIDASTNVLSVGAALLMIFRYREQWLFYILLNILTVTMWFIRFTE
                     GGNESIMMMCMWVFFFLNSLYGYWNWSRGAE"
BASE COUNT         1153 a          793 c          832 g         1081 t
ORIGIN      
        1 agtatgtttt gacctgctgt tccggtgttc caatcaaatg agagatgctt gcttcctgat
       61 tttctgcagt agaaaacagt gcaatctcct ccgcgttcag tgcataaatt cctgttctgg
      121 ggaagggatt gaacatttct ccgtagagtt ttttcagtcc atcactgtcg tcagttgaga
      181 tatttttcat ggaatctgtg aatttattca tgacagaaaa ttcattcgga tctgtgtttg
      241 atgcgccgag attcagtctg ttctggcatt gggaaacagt atcgcctggg ctgcagtggt
      301 cgagtcccag actgtggccg aattcgtggg tgaggacggt gttcagaata ttcggatcag
      361 aaagttcatt tgaatactca gaattgatct gaatgtcact gtgcagaatt tcatccccgc
      421 atgaccagag aaaactgcag gaagttcctg tgcagtccgg gtcggctccc tgtgacagat
      481 acagtgtatc ctcctttgac agtgagtttt ttgcagagtc agaacagctg atttctgacg
      541 gcacaagagg agaacgttca gtggacagtg ccttgactga gtagctgacc ttcgcactcg
      601 aattttgaaa ccaggaaagg gaggaatttt ccactgtgct gttccagaca gaagatgtaa
      661 tccccggagg gaaaacagac tgatcaacgt ttacccgtat gatcggaaaa ttacccggtc
      721 tgcgcggtct tgagttcggc gactggaaca tgaaattgca gacgctgatg cagatgaatg
      781 ataaaataat gtatttggtg tgtttcataa tttttctcct tttttggggg tcggtcggtt
      841 tttcagacct cgaggttttg aaaacctcga gggctgaact ccaattaaaa actgatcttc
      901 cccatctctc cgcgagtagg tgcataacgg tttccgtatt ttttaaggaa tacaagacag
      961 gattctcctg cagtgaattc cggcattccc ttgactttga gagtggagga attgactgtc
     1021 ccgcccggag tctgaatact gaattcagga gtgctgacct gacctctaag tctgtcttcc
     1081 actgtgagag tgttctgact gtagactgta tttttaccgt agatattctg actgacaaag
     1141 ggagagactg attttacagt tccctgaaag atgaggtcgg attcgcttgt cactgtattg
     1201 cttctctgtc cgagagcatc gcggattctt ccatcctgca caggggtcat actgatgacc
     1261 gaggttgtca gaagtgtggg atctaaaata taccctcttt ccgggatata aaaaatggaa
     1321 gtattcggat cgaagtcaag aaatacttct gtgagtcttc cgcctctgag agcaaacggc
     1381 cccatgaacc gaagcacgga ctgacccgca tcttctattg tcagaggaag aaactgcccg
     1441 tctatctgaa tctcctgctt tgtgcccaga gtcagttcaa agaaagcata gtccccttga
     1501 ggaaccatat tgtgtcccat cagtccgact gcaccgtttc ggagttccag aagttcaaaa
     1561 agggtcggct tctcatttag agatattttt tcattataat tatttactgc agaaagtttt
     1621 gtcattgtga ggagtctgtt caattccggg agatgcctga accactccac gcttcctgtc
     1681 caagacattg atgtcagaag ccaaaagtgt aaaatctgta ctctttttgt tcagcgaaaa
     1741 gagtcttttg caccccccaa ccaaaattat tagaatcatt acaagaaaac tggatctttt
     1801 tctcatttcg ttcattttcc tttcgaatac gcaaaaacgc ttgaaaaaga catatatatc
     1861 tgtttttagt tttgtcaagt atttcttagg gatcagggct cggagttggg caaaaaagca
     1921 aatgagtttt cattgaaata ttttttcatt ttttgtatta ttttatcatt aaaaagaatc
     1981 ttcaccattc cctattctct ttgaaaatcc gtttaacctt aaggttttaa attctgaaaa
     2041 aatagaaaaa atatgataaa tttctttgtt ttctttaccc tttcagaggt ctaattcaca
     2101 tcttaaaaga gaaagtattt atagaatgct ttcgcagatc agaaacttcc aagatgctga
     2161 atgtttttct ggaacgaggc ttggtctgat gcagtaacat aaaactatag aggaaaagat
     2221 atgaacaaca aacaaaaagc agcacagcag tacgactccc aaagtgcaag cttctcaaag
     2281 atcataaagc caatcacctt ggtaatcaca atttgtattg cagtcgcctt taccgaatta
     2341 tccgcacaga tgccacgatt tagtaaaacc gtaatcctaa aaaacggaat tgtctttgaa
     2401 ggtgtagacg caatacgtac agaggaattg ttcacgataa cgacccccga aggtatgaca
     2461 aaagtctacc aaagtaaaga aatttcaata attacatggg atggttcgaa tgatttgaag
     2521 aatgctccgc aaaaaccgag caacagaaaa tggagtgagg accaaggtta tatgaattgg
     2581 aaagatgcta tgaaaaaatg cgggagcatt ggtatgcggc tgccgaaacc tcaagagtta
     2641 aaggaagcat atgcagcagg tattactgaa tcttggaaat cagatgcccg ttattactgg
     2701 tcgtctgaac gttctaaaga agacaactgg attaaatttt tcaatatcta cactgggatc
     2761 accggcagca actttgaaac cagctatcac aaagttcgct gcttgataag tgactacata
     2821 tggagtgaag accaaggcag aatgaattgg gaagatgcta agaaaaaatg tgaaagcatt
     2881 ggaatgcggc tgccgacaac cgatgaatta aaggaagcat atgcagctgg gattacaaaa
     2941 tcttggaaaa aaatttccac cgataactat gacatctggt cttctgaccc aggtcgtgat
     3001 gacaccgcaa tagctcttga taacgacact ggagaggcat tacccaacga tcgtgaagga
     3061 aaaaaccccg ttcgctgctt gcattaaggc gtgtacaggt tttatttggt catttactcc
     3121 actgccttct caggagtgga gtattgactt gcggtgggca gctgtcctat tcagcacccc
     3181 gactccagtt ccaataccca tacagagaat tcagaaaaaa gaaaacccac atacacatca
     3241 tcatgatgct ttcatttcct ccttcagtga atcggataaa ccacattgtc acagtcagaa
     3301 tattcagaag gatgtagaag agccactgct ccctgtaccg aaagatcatc agaagagctg
     3361 ctcccacgga aagcacattt gtagaggcat caatataggg agtattctgt cctgcaatga
     3421 atgaaagact ccatccgaaa aggaaagtca gaagaaatgc acctgcaaga atatacaata
     3481 tattcatcag tttcattttt ttcatgatga ctgtcccgtc cagaatcctg tctttccaga
     3541 gaaaaatgcc tgcgatattg acagggatga agaaaatcag attcagtccg acttcaccga
     3601 aaagaccgtt gagccaggca atataggcat aagcggccga attcagtatg ccgaatataa
     3661 aattcccgat atgaccgcgt gcggcaagca caacacagat gagaccggaa acaagtgcag
     3721 taatgctgat tattttttcc atggattata gattattgta taaattcata aaaccttcat
     3781 ttggaactgt cttttctgga aacagaggat ctgccaattc tttcctgatc ccctctacaa
     3841 agtacatatc gatttcacc
//
LOCUS       DAOQGJ010000111         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13204_length_3848_cov_3.367519, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000111 DAOQGJ010000000
VERSION     DAOQGJ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13204_length_3848_cov_3.367519"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..1354
                     /locus_tag="PK683_02690"
     CDS             542..1354
                     /locus_tag="PK683_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07383.1"
                     /translation="MNFQDKMRERLSEYKKKKFPDVQDGLYQGRPYGHIFPKGEQNRN
                     LIEYYHDDILKNPHYRKEDLHIYFNHLNSTEALSFNFFFPLLIEKKLELLTSRLTLEE
                     KAVNYETAVFEKPGIDFEHFSDSIFDFYFETAAGKKICFRTKYTETGFGSAEDDVLHS
                     ELFEYVYKNYLSVITEKYRKKTEFLQRFQICRTLLHLDQNTILVIIYPRENHKIREEA
                     EKAANEILVPELRSCLTLFTWEELTEYILKNSVSPKKFQNQYKEFAEKYFPE"
     gene            complement(1364..1897)
                     /locus_tag="PK683_02695"
     CDS             complement(1364..1897)
                     /locus_tag="PK683_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH07384.1"
                     /translation="MSDAEYLDMERNSPEKHEFYFGEIYAMAGAKENHNLISLIHIQF
                     KGRPCCVYPSDLRVVVDEYSHYVYPDLVLVCGERKFLDKSNDSLLNPSAVIEVLSDST
                     ETNDRGKKFESYRNIISLQEYMLVSSERKKNELFSKSPEGKWVLSESGKERTIDISSL
                     NLHISVSDVYEKIEFEN"
     gene            complement(1946..2659)
                     /locus_tag="PK683_02700"
     CDS             complement(1946..2659)
                     /locus_tag="PK683_02700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNH07385.1"
                     /translation="MTSKSSDQKFILNVGSLQIQIVRKKIKRLYLSVKAPNAAIIMNV
                     PLRISLKEIIGFAESRLEWMQFHIDRILNTEWVPEQKFQSGEKHYFLGIKYPLKVTTV
                     SGRERLAFENGVFEMYVKSRSSKKKREKLMEEWYRVRVAEILPELIFKWENRMGLKIK
                     GFTVKKLRRTWGICHVKDRTISFSLGLGKVPMEGIEYVVVHELVHLFELHHNRRFYSL
                     MTKYLPTWRKDREKLNSLN"
     gene            3130..3255
                     /locus_tag="PK683_02705"
     CDS             3130..3255
                     /locus_tag="PK683_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07386.1"
                     /translation="MGHLPIWYLICGIFVIAVGLTFMARGFAGMIAEQIRKNEGK"
BASE COUNT         1110 a          772 c          740 g         1226 t
ORIGIN      
        1 gcagctgtta tcataatcgc ctgcatttat aaagtatttt cctttatctc tgctcttgag
       61 cctattcgga agcagaggat gaattattga ccgtcatcct aaaacggatt ttcggcagag
      121 atggagcctg cagatgatag aacgagaata ttatataagc ctttattatt gtatcccttt
      181 tcatttttcg ctccatccgc attttcattt cagtcaatcc atttgtcctg cgaatgaact
      241 ttctctgaac attgaaacag gcttactgtt tcggcttttc tggtaatttt ccccgaactg
      301 gaattctgcg gattcagact ccgtaataat cagttttgcg gtctgaaaac aaagactttt
      361 gattctgcat attcctctgc tgtctcggcg agatattgga caattacttg aatagaggag
      421 atcagtgatt gattttggat gaacattttt gtgcaaattc tcgaatttgt cctgaggaca
      481 gtgcaatttg attgcagaaa atgaacttgt ttcagtcaaa gtattcctga aggtaatttg
      541 gatgaatttt caggataaga tgcgggaaag actttccgaa tataagaaga agaaatttcc
      601 ggatgttcag gacggactgt accagggaag accctacggg catatctttc cgaaggggga
      661 acagaacagg aatctgatag agtactatca tgatgatata ttaaaaaatc ctcattacag
      721 aaaggaagat cttcatattt actttaacca tctgaattcc actgaggctc tttctttcaa
      781 tttctttttt cctctgctca ttgagaaaaa actggaactt ctgacttcca ggctgactct
      841 cgaagaaaag gcagtaaatt atgaaactgc cgtttttgaa aaaccgggaa tagactttga
      901 gcatttttcg gattccatct tcgattttta ttttgaaact gccgccggaa agaagatctg
      961 tttcaggaca aaatatacag agacaggatt cggatcagca gaagatgatg tacttcattc
     1021 tgaactcttc gaatatgtat ataaaaatta tctctctgta attacggaaa aatacagaaa
     1081 aaaaacagaa tttctccaga gatttcagat ttgcagaaca cttcttcatt tggatcagaa
     1141 caccatcctg gtcatcattt atccaaggga aaatcataag atcagagagg aggcagaaaa
     1201 ggccgcaaat gaaatacttg ttcctgagct cagatcctgt ctgacacttt ttacctggga
     1261 agagctgact gagtatatac tgaaaaattc agtcagtcct aaaaaatttc agaatcagta
     1321 taaggagttt gctgaaaaat actttcctga atgaggtttc tgttcagttt tcaaattcaa
     1381 ttttttcata tacatccgaa actgaaatat ggagattcaa tgaggaaatg tctattgtcc
     1441 tttcttttcc gctctcagaa agaacccatt tcccttctgg ggattttgaa aaaagttcat
     1501 ttttctttct ttcagatgat acaagcatat attcttgtaa tgatattata ttcctgtaag
     1561 attcaaattt ttttcctctg tcattggttt ctgttgaatc agaaagaact tcaatgacag
     1621 cacttggatt taaaagactg tcgttcgatt tgtccaggaa cttcctttct ccgcagacca
     1681 ggactaaatc aggatagaca taatggctgt attcatcgac gacaacccgc agatctgaag
     1741 ggtagacgca gcagggacgt cccttgaact gaatatgaat cagtgaaatc agattgtgat
     1801 tctcttttgc tcctgccatt gcataaattt ctccaaaata aaattcatgt ttctccggag
     1861 aatttctttc catatccaga tactcagcat cagacaaagt ctaaattttt cagtcaagca
     1921 taaataatga taaaatgcaa ggagttcagt tgaggctgtt cagcttttct ctgtcttttc
     1981 tccaggtcgg aaggtatttt gtcatcagtg aataaaaacg gcggttatga tgaagttcaa
     2041 aaagatggac aagttcatga acaactacat attcaattcc ttccatcggc accttgccca
     2101 gtccgagact gaaagatatc gtcctgtctt ttacgtggca gattccccag gttcttctaa
     2161 gcttttttac tgtaaaacct tttatcttca gtcccatcct gttttcccat ttgaagatca
     2221 gctcaggaag aatttctgca acccttaccc ggtaccattc ttccatgagt ttctctcttt
     2281 tctttttgga ggatctggat tttacataca tttcaaatac tccgttttcg aaagccagac
     2341 gttctcttcc tgaaacagtt gtgactttta aaggatattt tatccctaga aaataatgct
     2401 tttcccctga ctgaaatttt tgttccggca cccattccgt attcagtatt ctgtctatat
     2461 gaaactgcat ccattcgagt cggctttctg caaatcctat gatttctttc agactgattc
     2521 taagaggaac gttcatgata atagcagcat tcggagcctt tactgaaaga taaagtcttt
     2581 tgattttttt tctgacaatc tgaatctgca gactgcctac gttcaggata aatttctgat
     2641 ctgaggattt ggaggtcatt ttttctgatc acagagacag tatattgtat ctgaatgctt
     2701 tccttttcgc gcggttcatt atctgcccgt cggtcattga atcattgttt tttatcgaaa
     2761 gtcggcttta tatttcttga aaaaatcgca aactgcgatc ataaatactg caactgtcct
     2821 aactttgaaa ataacattca cactttcctt gtatgcggat atgtctgaag aagtgcaatt
     2881 cccagactga atacacatcc aataaaaaga attccggtca gactcatttc aatctccaat
     2941 tgactccatt atgctcattt ctcaatttaa acaagcgaaa aaattgaatc ccttttcaga
     3001 atgccggggg cagggagctg actgtttact gaggcggatc agaacaaatc agttctgatg
     3061 tgcgaggaaa tccattttct gctttacagc aagcaggttt ttcttagaat atagtcgacg
     3121 gaggctacaa tgggacactt gcctatctgg tatctcatct gcggaatctt tgtaattgct
     3181 gtaggattga cttttatggc aagagggttt gcaggaatga ttgctgaaca gattcgaaag
     3241 aacgaaggaa aatgatcaat ctcaattcgc tgctttttct gcttggagcg atctggattg
     3301 gattggcatc cgctcttcta ttttcatata atgaatacaa accgtattcg gagcctatta
     3361 tgatcgtact tactgtatca actgcaatta tttctttcta cgatttcata agaaaaaaat
     3421 aattatagaa tttactgaac cgctgaagcc tttttcagaa taccggcgag acctcacccc
     3481 ggcttccagc ccgcccctct ccataacgtt taaagatttc gttatggaga ggggcctgag
     3541 gcggatgcat tgctgataat aagaaaccat ttattcggat tccccttctc tataacgtaa
     3601 ggaatggcca cccttgctcg caaaattttg atgttgctcg caaccaggca agcctcaccg
     3661 caatgcggtt cgctggggct aggggatagc gtgagcgagg taaaactgtc aagtgtcaag
     3721 ttctaaaata ggttgtcatt ttcagccacc aagtaaaatg cctgcaaggg ttcgcttggg
     3781 cgacgtttcg aaacgtcgct tctcagggca aacctgaaca gaatgctctg atcagaacgc
     3841 aggcggga
//
LOCUS       DAOQGJ010000112         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13239_length_3843_cov_2.617740, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000112 DAOQGJ010000000
VERSION     DAOQGJ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13239_length_3843_cov_2.617740"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..476
                     /locus_tag="PK683_02710"
     CDS             105..476
                     /locus_tag="PK683_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07387.1"
                     /translation="MKFLKILILALAGFNTVSCVYSQARILGPTNQQTQYILTSEDFK
                     ILGPVEAEGEYMAILGMVGLGGNGYDILQKKAQQMGGDDIMNFQVDLEGTAVLLIVYD
                     KAKWRARAMAVKYTDKVKARQ"
     gene            complement(485..817)
                     /locus_tag="PK683_02715"
     CDS             complement(485..817)
                     /locus_tag="PK683_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07388.1"
                     /translation="MAGADKLSERQVQLIQSAAGILMEHLYMSKEEAVAVIAESLREE
                     LKAGGLTFEFLEVNSLSYRQTFIRNLVYRVEKKLENIKTLRKEKIKSAVNIFLQMLHY
                     SWMNEEKT"
     gene            1826..2521
                     /locus_tag="PK683_02720"
     CDS             1826..2521
                     /locus_tag="PK683_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH07389.1"
                     /translation="MLSKSQARMFFLLGTVLFSGLFLFLTVDTMQKIPDQTKSANITD
                     SVKKGKLLFDKNNCMGCHTILGEGAYYAPELTKVHERRGPEWIRVFMKDPEAMYPGQR
                     KMVKYNFSEEELSDLISFFKWIGEMDLNGFPPKPNITVNGADQTNAGSAGTAPKKFTA
                     LCTACHSVGGTGGKVGPALDTAGKRLGKDFISGWLKDPQKVRPGTKMPKLPLSDSEIA
                     EITDYLIQMKGKE"
     gene            2524..>3843
                     /locus_tag="PK683_02725"
     CDS             2524..>3843
                     /locus_tag="PK683_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNH07390.1"
                     /translation="MKFKSQKVAYWFFAVCMLLLSLQIIYGFIMGFARMGYDVLHPHI
                     AFNTARAVHTNLLVVWLLSGFMGAAYYIIPDESEQDLYSVPLAYVQLVSLVLVGVIAL
                     IGFHINWWEGRKFLEIPRPLDFLVVINVLMFLFNIGMTIFRAKRYTTSSIVLYLGLFS
                     AALLYLPGMIYFDSQTLDSYFRWWVVHLWVEGVWELIMGSILAYLLIKLTGVDREVIE
                     KWLYVIVGLTFLSGILGTGHHYYYIGTPKYWLWIGGLFSALEPLAFLAMAVFAVTMYR
                     RSGRNHPNKISLYWTVGCAIMSFVGAGFLGFAHTLPQVNLWTHGTLVTAMHGHLAFWG
                     AYAMIVLGIIAYAMPRMTGRKLWNSPSGIFAFWLSNIGMIGMTGAFAVAGIAQVYLER
                     KMGLDFLTVQKEIEVHFLGLVLAASLFTLGIILYIYNFIKYGLPNDEM"
BASE COUNT         1079 a          816 c          846 g         1102 t
ORIGIN      
        1 aaaaagtctt tcagtttgga gtgaattgac aaaattgcaa taagagaatt tacaaaactc
       61 aaaaggttta taaaatatat tccagtttcc aatttggagg gaaaatgaaa tttctgaaaa
      121 ttttaatttt ggctctggca ggcttcaata ccgtatcctg cgtgtattct caggcaagga
      181 tcctcggtcc gacaaaccag cagactcagt atatactgac atctgaggat tttaagattc
      241 tcggtcccgt ggaagcagag ggcgaatata tggcaatcct gggaatggta ggtctgggcg
      301 gaaacggata cgatattctg cagaaaaaag cgcagcagat gggcggagac gatattatga
      361 actttcaggt ggatctggaa ggaactgccg tgcttctgat tgtatacgac aaggcaaaat
      421 ggcgtgctcg ggcaatggcc gtcaaataca cggacaaggt gaaggccaga cagtagtcgt
      481 tttttcaagt tttttcttcg ttcatccagc tgtaatgaag catctgcagg aaaatattga
      541 ctgcagattt gattttttcc ttccgcaggg tcttgatgtt ctccagtttt ttctccactc
      601 tgtaaaccag attacggatg aaagtctgac ggtacgaaag agaattcact tcgaggaatt
      661 caaatgtcag tccgccagcc ttcagctctt ctctaagaga ttctgcaatg acagcgacag
      721 cttcttcctt ggacatataa agatgttcca tcagtattcc cgcagcgctc tgaatgagct
      781 gcacctgtct ttcgctgagt ttatccgcac cggccattct tcaaatgtcc gccgatgcgg
      841 atttctgagc aattcttttt tatggggaga gacgggatcg gagttccagg gctcagcgaa
      901 ctgcattcga tccgtcagaa gggagagatg aaacatttcc ggaacatagg cgagcctaca
      961 ggattgaaat attatatcat tatataatat atgatacttt tcgaagattc aggacagtaa
     1021 aaatatattt ctttatatcc tctccgttca tccatagact caggcataat cattctgtcc
     1081 gctgaatcgc atctagccag tgattccccc tcggctctgc gagctgcctg gcatacctgc
     1141 gtccccttcg ccttcgggct gccgaggact ccgcatattc tttactgcat ttcaacaagc
     1201 cggtatagaa caaattcaga taagattcct gttccgaaga ttcattcaac gaacagaatc
     1261 tttcaaatca aatatgtcta aaaactcttt tgatcatttt tctccagtaa atgtcaattt
     1321 taatttcagc cgattacttt ttgaacttct aatgaataaa tgccggagaa caatatgtgc
     1381 atccaaccgt ttccatcctg aaatcgctga aaaaagaacg gcagactcag ttgattccag
     1441 actcaatttc cacaggaaat ccccgttcaa atgattcaga ctggattcta ttccactgaa
     1501 ccgattgcac ggacaattca aaaacccccg tcatcaaaga gcagatcatc attatgttta
     1561 cattttttat aattatagaa ttacaaaata aattcattat atgtcagcag gaaaattcga
     1621 cggaaaatat ccggaagaac cgctctctct gaaggtatga cttgaaaaaa caggctgtcc
     1681 gttattgacc ttcatcaatt tttttgaaaa tcagttgatg cagatcaata tttatttata
     1741 ttttttttac attaacagac atttttagtt gaaatgcaca aaaataagtg tgcgaatatt
     1801 ctcaaacaga ggttcaggag gagtgatgct ttccaaatca caggcacgta tgttcttcct
     1861 tttagggact gttctctttt cgggtttgtt tctttttctc actgtggata caatgcagaa
     1921 aattccggat cagacaaaat ccgcaaacat cacggattca gtcaaaaaag gaaaacttct
     1981 tttcgataaa aacaactgta tgggatgcca tacgatactc ggggaaggag cctattacgc
     2041 tcctgagctg acaaaggtgc atgaaagaag aggtcctgag tggatcagag tcttcatgaa
     2101 agatcctgag gctatgtatc cgggacagag aaagatggtg aagtacaatt tcagtgaaga
     2161 agaactctct gatctgatat ccttcttcaa atggatagga gaaatggatc tgaacggatt
     2221 cccgcctaag ccgaacatta cggtcaacgg agccgatcag acaaatgcag gttctgcagg
     2281 aactgcaccg aaaaaattta ccgcactctg cactgcctgc cattcggtcg gcggaacggg
     2341 aggaaaggtc ggacctgcac tcgatactgc agggaaaaga ctgggaaagg actttatatc
     2401 cggctggctg aaggatccgc agaaagtcag gccgggcaca aaaatgccaa agcttcctct
     2461 ctcggattcg gaaattgctg aaatcacaga ttatctcatt cagatgaaag gaaaggagta
     2521 agtatgaaat tcaagtcaca gaaggttgca tactggtttt ttgcagtctg catgctgctc
     2581 ttaagcctgc agattatcta cggttttatc atgggttttg ccagaatggg atatgatgta
     2641 ttacatccgc acattgcatt caacacagca agggcagttc atacgaatct gcttgtagtc
     2701 tggcttctct ccggttttat gggagctgca tattatatca tccctgatga atcagagcag
     2761 gatctctatt ccgtccccct ggcctatgtt cagcttgtga gtctggttct ggtcggcgtc
     2821 attgctctca tcggatttca tatcaactgg tgggaaggaa gaaagtttct cgagattccg
     2881 agaccgctcg acttccttgt cgtaatcaat gtactgatgt tccttttcaa tatagggatg
     2941 actatattca gagcgaaaag atacactaca agttccatcg ttctctacct cggtcttttt
     3001 tcggctgcac ttctttatct ccccgggatg atctactttg acagtcagac attggattcc
     3061 tatttccgct ggtgggtagt tcatctttgg gtggaaggag tctgggaact gattatggga
     3121 tcgatacttg cctatcttct gatcaagctc actggtgtag acagagaggt cattgaaaaa
     3181 tggctctatg tgattgtagg tctgactttt ctttcaggga ttctaggaac gggacatcac
     3241 tactactata tcggaactcc caagtactgg ctctggatcg gagggctgtt ctctgcactg
     3301 gaacctctcg cctttcttgc aatggcagta tttgcagtca caatgtacag aagaagcgga
     3361 agaaatcatc cgaacaaaat ttcactctac tggactgtgg gatgtgccat catgtctttt
     3421 gtaggcgcag gctttttagg ttttgctcat acgcttcctc aggtcaatct ctggacacac
     3481 ggaacacttg ttaccgcaat gcacggacat ctcgcattct ggggtgccta cgcaatgata
     3541 gttctgggaa tcatcgccta cgccatgcct cggatgactg gaagaaaact ctggaacagt
     3601 ccttccggaa tattcgcatt ctggctttca aacatcggaa tgattggaat gacaggtgca
     3661 tttgcggtag caggaattgc acaggtatac ctggaaagaa aaatgggact ggattttctc
     3721 acagtccaga aggaaattga agtgcatttc ctcgggcttg ttctggcagc ttcccttttc
     3781 actctgggaa tcatactgta tatatataat ttcattaaat acggacttcc caatgatgaa
     3841 atg
//
LOCUS       DAOQGJ010000113         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13313_length_3828_cov_3.628147, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000113 DAOQGJ010000000
VERSION     DAOQGJ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13313_length_3828_cov_3.628147"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..>385)
                     /locus_tag="PK683_02730"
     CDS             complement(110..>385)
                     /locus_tag="PK683_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family transcriptional regulator"
                     /protein_id="HNH07391.1"
                     /translation="KERNIKFQSIDLNVKGFAKGELQRITKKLKADDLLDKEGKPFQN
                     SQYKYMQVDAFELILEKPEFTKTPVVSNGPESTVGYCPEVWEKWIKK"
     assembly_gap    386..395
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(490..1659)
                     /locus_tag="PK683_02735"
     CDS             complement(490..1659)
                     /locus_tag="PK683_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNH07392.1"
                     /translation="MPVLPDWINFNFPAKIHLEVDCAHKCGQFIKHVGSRVLIVATQK
                     ELQNSIDLSSVKKSIEQQTEGVILYDNIPGVATYKELDTAAHFARQAQVNCIVGYGGS
                     ESINAAKALSVLASNDFFAEEMMTGKKPLKNKPLPLVIIPIMPLMGLECNPFCFIQED
                     GTSVRKYFSHQSLFPELIISDPKIGFHMSSSELGKAGTAIMSAAMDSIFSKYSNEITN
                     ASALRAIELVAKNLIPAIRDPKNLAPKSALYAASLLAGISSSINSLGINFALALSATS
                     LTKLDILQAMSIFLPHVMEYNLTSSAGKYVMIARALDEDTNNISIIEAAIRAVEGVRK
                     IYFELKIPQKLAEYEVKKIELPSIAAMAVQFPFLDSLLKELPKNEVETILVAAYN"
     gene            complement(1681..2004)
                     /locus_tag="PK683_02740"
     CDS             complement(1681..2004)
                     /locus_tag="PK683_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07393.1"
                     /translation="MLINKIKSLGQSLEAKKASAVKSTASFISNDNIAISEAAKKKSV
                     EMQFQADVDSITQKALIVRTDELRANKIKDVKSKLLSGYYDNPSNDILKRTADSLLDS
                     FLSLN"
     gene            complement(2026..2739)
                     /gene="rsmI"
                     /locus_tag="PK683_02745"
     CDS             complement(2026..2739)
                     /gene="rsmI"
                     /locus_tag="PK683_02745"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNH07394.1"
                     /translation="MKRSSGTLYVVGTPIGNLEDITYRAVKTLQECSLILAENGTNSK
                     KLALSLKIETPIKQLYASTKEEDLDWLWKIFEEGKDVSYISDAGTPGVSDPGANLVRY
                     CRKRDIPVSPIPGPSSLTALLSVSGWQTNPAYFLGFLPDKNSQKKNLLQKFVSEECLI
                     VVFESVHRIQNLLELLREVYPGAEILVGRELTKSYEEIILISPEEPIPKVTAKGEFAV
                     IVNNRLKKIAKERKATTDI"
     gene            complement(2736..3455)
                     /locus_tag="PK683_02750"
     CDS             complement(2736..3455)
                     /locus_tag="PK683_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07395.1"
                     /translation="MLIRKAFILCAVFVLNCGTNTNTPVIPFIFFAPPGIPQIAGIIP
                     TTEIPIPASLIIDGLGDKAPVKPEFVLKYYVTNTEQNFVGYNLSITSAVPTLADTMAG
                     AAVYTESGILPSFPHLATEANTAKSKIVKRKIANRIPPPGMYPFQHCEVFTFTMRAYL
                     NNGFTSQPSASVAACASPYPYKCPIGSSCNPTACSLSTCTADVKVTCPVGTYCNPCLI
                     SGQEGNGCECPAGTSPPGCNP"
BASE COUNT         1038 a          808 c          809 g         1163 t
ORIGIN      
        1 ccgggacaat ggcaagcttc aatggaaatc tttacgtggg atggtcagag caggcgagcg
       61 gaaatgagta caatatctat ataagccgaa tggcatattg aaattgtaat tatttcttaa
      121 tccatttttc ccagacttct ggacagtatc cgacagttga ttccggaccg ttggatacaa
      181 ctggagtttt tgtgaattcc ggtttttcca gaatcagctc aaaagcgtcc acctgcatat
      241 atttgtattg gctgttctga aaaggttttc cttctttgtc cagaagatca tctgctttca
      301 gtttctttgt gattctctga agctcgcctt ttgcaaaacc cttcacgttc agatcaatag
      361 actggaattt aatgttcctt tccttnnnnn nnnnntgaaa aaacgctctg ccttttttgt
      421 atcactgcat ttttttgtgc cgaaaatctg gatattcata agataatgat tttttaataa
      481 taggcagtat taattataag ccgcgacaag aattgtttcc acttcattct ttgggagttc
      541 cttcagaaga ctgtctaaaa acggaaactg caccgccatt gccgcgatgc ttggaagctc
      601 aattttcttt acttcatatt ctgcaagttt ctgaggaatc ttcagttcaa aataaatctt
      661 cctgacacct tccactgcac ggatcgcagc ttcaatgatg gaaatattgt ttgtgtcctc
      721 atcaagagca cgtgcaatca tcacatactt tcctgcggac gaagtcaggt tgtattccat
      781 tacgtgagga aggaaaatag acattgcctg aaggatgtca agcttagtaa gactggttgc
      841 agacagtgca agtgcaaaat tgatcccaag tgaattgatg gaacttgata ttcccgctaa
      901 cagacttgca gcgtacaatg cacttttagg agcaaggttt ttaggatcac ggatcgcagg
      961 gataagattc tttgcgacaa gctcaatagc acgaagagca gaagcatttg taatttcatt
     1021 ggaatacttg gaaaaaatag aatccattgc agcagacata attgcagtgc ctgcttttcc
     1081 aagttcagaa ctgctcatat gaaacccgat ttttggatca gaaataatca gttcaggaaa
     1141 aagactttgg tgagagaagt attttctgac agatgtacca tcctcctgaa taaaacagaa
     1201 aggattgcat tccagaccca tcagaggcat aatagggata atgactaaag gaagaggttt
     1261 gttctttaag ggctttttgc ctgtcatcat ttcttccgca aagaagtcgt tgcttgccaa
     1321 tacggagagt gcctttgctg cattgatgga ttcgctgcca ccgtaaccta caatacagtt
     1381 tacctgtgcc tgtctggcaa aatgagctgc tgtatccagt tctttatagg tagcaacacc
     1441 gggaatatta tcgtaaagaa taactccctc agtctgctgc tcgatgcttt tttttacact
     1501 gctgagatca atgctgtttt gaagttcttt ttgagtggca acaatgagca ctcttgagcc
     1561 tacgtgttta ataaactgcc cgcacttgtg tgcacagtca acttccaaat gtatcttggc
     1621 aggaaagtta aaatttatcc aatcaggaag tacgggcatg tcttaaaagg aaagtaccag
     1681 tcagtttaaa cttaggaaag aatctaacag actgtctgca gttcttttca gaatatcatt
     1741 tgaaggattg tcataatatc cgcttagaag tttggatttt acatccttga ttttatttgc
     1801 tctaagctca tctgttctaa cgatcagtgc tttctgcgta atggaatcca catctgcctg
     1861 aaactgcatt tctactgatt tttttttggc cgcttctgag atagcaatat tgtcattgct
     1921 gataaaagag gcggtagact tcacagcaga cgctttcttg gcttctaaac tctggcccaa
     1981 gcttttgatc ttgtttataa gcattatgtt ctacctctct taatcttata tatcggtcgt
     2041 agcctttctc tctttagcta tttttttgag ccggttattc actatcacag cgaattcgcc
     2101 ctttgctgta accttcggaa taggttcttc cggtgatatc aatataattt cctcataaga
     2161 ctttgtcagc tcacgaccca ctagaatttc tgctccagga taaacttcac gaagaagttc
     2221 aagcagattc tgtattctat ggacggattc aaatacaaca atcagacatt cctctgatac
     2281 aaatttttgc agaaggtttt ttttctgaga atttttatcc ggcaaaaagc ccaagaaata
     2341 tgcaggattt gtctgccagc cggaaacgga aagcagtgca gtcaatgaac tgggcccagg
     2401 aatcggagaa acgggaatgt ctctttttct gcagtacctg actagatttg ctccgggatc
     2461 agacactccg ggagttcctg catcagagat atacgataca tccttccctt cttcaaatat
     2521 tttccaaagc cagtccaaat cctcctcttt agtggaagca taaagctgct ttatcggggt
     2581 ttcaattttc agactgagag ccagtttttt ggagttcgtc ccgttttctg caagaatcag
     2641 actgcactct tgaagcgttt tgactgccct gtatgtaatg tcctccagat tgccgatggg
     2701 agttcccacg acataaaggg tgccggagga ccgtttcaag ggttgcagcc gggcggagaa
     2761 gtgcctgcag ggcactcaca tccgtttcct tcctgaccgg aaatcagaca aggattgcaa
     2821 taagttccta caggacaggt cactttcaca tccgcggtgc aagttgagag tgaacaggct
     2881 gtcggattgc agcttgaacc gataggacat ttatagggat aaggacttgc gcaggcagca
     2941 acggaagctg aaggctggga tgtaaatcca ttgttcaaat acgccctcat tgtgaacgta
     3001 aatacctcac agtgctgaaa cggatacatt cctggaggcg gtatcctgtt ggcaattttt
     3061 cttttgacaa ttttggattt tgctgtattt gcctctgttg ccaggtgggg aaatgaagga
     3121 agaattccgg actcagtgta aactgcggcc cctgccatcg tatctgccag agtaggcact
     3181 gcggatgtga tgcttaaatt atatcccaca aaattctgct ctgtatttgt aacgtagtat
     3241 ttcagaacaa actccggttt gacgggagct ttgtctccta aaccgtcaat gatcagactt
     3301 gccgggattg gaatctccgt tgtaggaata attcctgcaa tttgagggat tccgggaggc
     3361 gcaaaaaaaa taaaaggaat caccggtgta ttcgtgtttg taccgcagtt caaaacaaag
     3421 acggcgcata gaatgaaagc ttttctaatc agcacttgta catcatgatt ttcagctgat
     3481 tctgcagtca agcagtactt tcttagcggt gcagtgagtt tattgaacct taatttccac
     3541 aaccgccttt gcaggcttcg tgccctgcga tgaaaattgt atcgctgcag ctttgaagtc
     3601 actgatcatt tcaaaggaag aaattacctc ttcaaaatcg aacagctaaa tcaatgtgct
     3661 ccaatatttc tgaacaggtt tattctgcag tcagcccgat aaattctgca aagacccagc
     3721 ctctcgggca ggaagagtac cagccggttt tggcattcac gtagtaccag cagttttccc
     3781 aattctgtac tttgactttt ttcgttgtcc tgccgatcgc tgtaagct
//
LOCUS       DAOQGJ010000114         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13316_length_3828_cov_2.971111, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000114 DAOQGJ010000000
VERSION     DAOQGJ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13316_length_3828_cov_2.971111"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /gene="ccrA"
                     /locus_tag="PK683_02755"
     CDS             <1..884
                     /gene="ccrA"
                     /locus_tag="PK683_02755"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HNH07396.1"
                     /translation="GIRDQNDPWVKAGNDPMFAPSQLIWGYETSWGSFAQFCKVQDHQ
                     CLPKPKHLTWEESAAYMLVGATAYRMLHNWAPNTVKKDDVVLIWGGAGGLGAMAIQIV
                     KAAGGVPIAMVSSDDKIDYCMKLGAKGVINRNKFKHWGALTSEINKPEKFAEWTKNAR
                     EFGKAIWDIAGKGNNPKIVFEHPGESTLPTSMFVCETGGMVVICAGTTGFNATVDLRY
                     LWMRQKRLQGSHFANDENSKGFNDLVIAGKVDPCLSRTFKFEETALSHQLMRENKHPH
                     GNMSILVGAPTTGLGKS"
     gene            complement(966..1553)
                     /locus_tag="PK683_02760"
     CDS             complement(966..1553)
                     /locus_tag="PK683_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH07397.1"
                     /translation="MNYIFSGEGCPVNMEGTYRIEGKKLHYTLTGENSSNCGSIDMKR
                     LKGICTFQILKDHPDFEKKLKCTGTIGSDPELYKDHPKEGTLRKIDGMEVALMGHRKG
                     IISEDAFIRSAPNTGAKPIECRLGSDIGKEKPRHTLPPKHTVTVMRRTKTKVKVQNWE
                     NYWYYVDASDDWYSNCQGWVFAEFVKLQKNELILP"
     gene            complement(1878..3464)
                     /locus_tag="PK683_02765"
     CDS             complement(1878..3464)
                     /locus_tag="PK683_02765"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07398.1"
                     /translation="MKKLPIGIQTFSKIIEDNCYYVDKTRIVSELAASGGYFFLSRPR
                     RFGKSLFLDTLKEAFSGNRDLFKGLYLEKNWNWELKYPVIKISFGNGAHSLEGLHSSF
                     EYNLRVSAEDHSVSLEGLHYDEKFKDLIRKISIKYNEKVVILIDEYDKPILDNLTYPE
                     TAVRIREELRSFYSVIKDSDAFVRFVFITGVSKFSKINLFSGLNNLQDITLDRNFSSI
                     CGYTQSELLNVFQDGLADVNMDELKLWYNGYNFLGEKVYNPFDILLYLKNKEFKNYWF
                     ETGNPSFLIELIHKNNYNSIDIENVEISEESLGSFDVDMIELETLLFQTGYLTIDKKY
                     NIGAKIRYRLKYPNMEVRMSLTEVILKFLTDSRKTVKIESELYRILEKNDLDAMQPLF
                     HSFFASIPHDWYRKNTMANYEGYYASIFYCYFTSLGLDVRTEDVTNQGQVDMTVFFED
                     RVYVFEFKVIELAGPGSALAQIKTKRYYEKYLSAFTGTQGDIAGTHGRASLRRENIYL
                     IGVEFSRDSRNIEVCEWEKL"
BASE COUNT         1059 a          879 c          791 g         1099 t
ORIGIN      
        1 gcggaatccg ggatcagaac gatccttggg tcaaggcagg aaatgatcct atgtttgctc
       61 cttctcagct catctggggt tatgaaacga gctggggctc ttttgcgcag ttctgcaagg
      121 ttcaggacca tcagtgcctt cctaagccga aacacctcac ttgggaagaa tcggcggcct
      181 atatgcttgt gggagctact gcttaccgta tgctccacaa ctgggctccg aacacagtca
      241 agaaggacga tgtagttctg atctggggcg gtgcaggcgg actgggtgca atggcgattc
      301 agatcgtgaa agcagccggt ggagttccta tcgcaatggt gagctctgac gacaaaatcg
      361 actactgtat gaaactcggc gcaaaaggtg tgatcaacag aaacaagttc aagcactggg
      421 gagcactgac ttccgaaatc aacaagccgg aaaaattcgc tgagtggacc aagaacgccc
      481 gcgaattcgg aaaagccatc tgggacatcg caggaaaagg aaacaatcct aagatcgttt
      541 tcgaacatcc aggtgaatcc actcttccta catccatgtt cgtctgcgaa acaggtggga
      601 tggtggtgat ctgtgcagga actactggat tcaatgcaac agtggatctg cgctacctct
      661 ggatgagaca gaagcgtctg cagggttctc attttgcaaa cgatgagaat tcaaaaggct
      721 tcaacgatct cgtgatcgcc ggcaaagtgg atccttgtct ttccagaaca ttcaagttcg
      781 aagaaaccgc tctctctcac cagctgatga gagaaaacaa acaccctcac ggcaatatgt
      841 ccatccttgt aggagctcct acaacaggac tcggcaagag ctgaggattt tgctcaccaa
      901 ccctgtctct gtcaagaggc ggggtttttg actgtcttac agtccggcag aaacttcaac
      961 tctcttcacg gcaggattag ttcatttttc tgcagtttta caaattctgc aaatacccag
     1021 ccttgacagt tagagtacca gtcatcgctt gcatccacat agtaccagta gttttcccag
     1081 ttctgcacct tgacctttgt tttagttctt ctcattacgg tgactgtatg cttgggcgga
     1141 agtgtatgtc tgggtttttc tttccctata tcacttccca gtctgcattc gattggtttt
     1201 gcgcctgtgt tcggagctga acggataaag gcatcctcac tgatgattcc tttgcgatgc
     1261 cccatcaggg caacttccat cccgtcaatt tttcgcaggg tcccttcttt tggatgatcc
     1321 ttatacagtt caggatcgga gccgatggtt cctgtgcatt tcagcttttt ttcaaagtca
     1381 ggatgatctt tcagtatctg aaaagtgcat attcctttca gccgtttcat atcaatcgaa
     1441 ccgcagttgc tggagttttc accagtaaga gtatagtgca gtttctttcc ctcaattcgg
     1501 tatgttcctt ccatatttac cggacaccct tcaccggaaa aaatataatt catccgagta
     1561 ccagagacag tcaaggacca gccaagacct gcatccgctt tccaaccgat ttctgaagtc
     1621 cacttgcccg aaaaatcatc ggccgaaatt ccaaaagccg tcattgtcag tgcgatgata
     1681 aacaaagatt ttattctatt catagaaaca actttatatc tgcatctgag actgtgaaca
     1741 tttttttgga ctgctcttct atacattttt ttttgcttag cagccgcgtt ttgattgtat
     1801 aacaaaccgg aaattggccc cttgaatata attaaaacag gagttcttgc cgacccgagc
     1861 acccaccttc aggcgtatca aagtttttcc cattcgcaga cttcaatatt tctgctgtct
     1921 ctcgaaaact ccacaccgat cagatagatg ttttcccgcc gtaaggaagc acggccgtgc
     1981 gtccccgcaa tatcaccttg cgtccctgtg aaggcagaca gatatttttc ataataacgc
     2041 ttagttttta tctgagccag agcagatcct ggaccggcga gctcaatgac cttgaattca
     2101 aatacataca ccctgtcttc aaaaaagacc gtcatatcga cctgcccctg attggtcaca
     2161 tcttcagtgc gcacatccag accgagagaa gtgaaatagc agtaaaatat agatgcatag
     2221 tatccttcat agttggccat tgtgttcttg cggtaccagt cgtgaggaat ggaggcaaaa
     2281 aaggaatgaa aaagaggctg cattgcatca agatcgtttt tctccagaat cctgtacagt
     2341 tcagattcta tcttcacagt ctttctgctg tccgtaagaa attttagaat tacttcagtc
     2401 aggctcattc ggacttccat attcggatac ttcaggcggt agcgtatttt ggcgccgata
     2461 ttgtattttt tatctatggt cagatatcct gtctgaaaaa gaagagtttc gagttcaatc
     2521 atatccacat caaaagatcc cagactttcc tccgagatct ccacattttc aatgtcaatt
     2581 gaattataat tatttttatg aatcagctca atcagaaatg agggatttcc tgtctcgaac
     2641 cagtaatttt taaattcctt atttttcagg tacagcagta tatcgaaagg attatagact
     2701 ttttctccca aaaaattata cccattgtac cataatttca attcatccat atttacatcg
     2761 gccagtccat cctgaaatac attgagaagc tctgactgtg tatatccgca gatagatgaa
     2821 aaattcctgt ccagtgtaat atcctgaaga ttattcaagc cgctgaaaag attgatcttg
     2881 gagaattttg atactcctgt aatgaatacg aaccggacaa aagcatcaga gtccttaatg
     2941 acggaataaa aacttctcaa ttcctccctg attctgacag ccgtttccgg ataagtaaga
     3001 ttgtcaagaa tcggtttgtc atattcatct ataagaatta caactttttc attatatttt
     3061 attgatattt tacggatcag atccttgaat ttttcatcat agtgaagtcc ttccaaactg
     3121 accgaatgat cctccgcaga gacgcgcaga ttgtattcga aggaggaatg aagtccttcc
     3181 agactgtgag ccccgtttcc aaaactgatt ttaatgacag gatatttcag ctcccagttc
     3241 cagttttttt ccagatacag tcctttgaac agatctcggt ttccggaaaa agcttccttg
     3301 agcgtgtcaa gaaaaagact cttgccgaat ctgcgcggac gggatagaaa aaaatacccc
     3361 cctgacgcgg caagctccga aacgattcta gtcttgtcta cataatagca gttgtcctca
     3421 atgattttgg aaaaagtctg aataccgatc ggaagtttct tcacagataa aaaatctcaa
     3481 agtatccttg tatgtcaaac agatcctgtt taggaattta cggaattgga tttctgatcc
     3541 aaaggactct ttcctaattt gacttttccg atgtctgggg aaaatccaga ttttgaagaa
     3601 gaagactttt catatctaac aatgatagat atgaaaatga caggaggtct acctggcaat
     3661 gctgtacaat tagcaaaaga ctttcatcta cgttatgatg aatgatgccc cgatacaatt
     3721 ttcactgcag gtcaccgagc gatgtaccga gcctgccgag gcatacaaac ccaatatgtt
     3781 cagggttttt cgtatggatt cgactcagct caccacaggt cgaggtgg
//
LOCUS       DAOQGJ010000115         3827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13321_length_3827_cov_2.690615, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000115 DAOQGJ010000000
VERSION     DAOQGJ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13321_length_3827_cov_2.690615"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="PK683_02770"
     CDS             complement(<1..1678)
                     /locus_tag="PK683_02770"
                     /inference="COORDINATES: protein motif:HMM:NF021424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNH07399.1"
                     /translation="MSIRVKLHHKTEYIYEKPVSIFPHVIRLRPAVHTRTPVLSYSLN
                     VLPKKHFLNWQQDPFGNYQARFVFLEKSKELSFEVDLTADMTVINPFDFFLEPNAERF
                     PFKYDDGLKLELLPYLYVNEESSKFNDFAASVPMDEKRSVDFLVEINQIVHKHVDYII
                     RMEPGVQTPEETLKIGKGSCRDSAWLLVQTLRKLGLAARFVSGYLIQLTADVKPLDGP
                     AGPSEDFTDLHAWTEVYLPGAGWVGLDPTSGLFAGEGHIPLACTPEPAGAAPLTGATE
                     KADVKFNYEMSVTRIQEKPRTTKPYTEGQWGKIYSLGAKIDSEMKETDVRLTMGGEPT
                     FVSALDMDGAEWNTEALGPTKKKYALDLLHRLKGKWGFGPLLHFGQGKWYPGESLPRW
                     ALACIWRKDGIPVWRDQALIADENLNLGMDHKHAYEFIKTLTSFLNITDRYVVPGYED
                     VYYYLWKEGTLPVNVDPFKKNLKDSEERKTLRKVLEQGLDKIAGFGLPLRWNSYESVW
                     ETGPRNFRRDRMYLIPGDSPMGYRLPLSSIPHSESYTYAEEPSSFEPKVPL"
     gene            2198..>3827
                     /locus_tag="PK683_02775"
     CDS             2198..>3827
                     /locus_tag="PK683_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH07400.1"
                     /translation="MKDSKSILFIEDSELPQIREIENLKKSGYDIIPVKSGEEALRSA
                     SANGTVNLVMMDMREDRKDLVQKILSERNLPILFVVSDSDIEYIDKVREFPNCTYMMK
                     NSDEFTLKSSVDAAFELFDANELKKENEESLKVTLQSIGDAVITADLNRKVSRMNASA
                     EALTGWSFKEAENKRLEDVFKTVNIDSFDSLKETVESVIKNGRTARFSEHAILTDRQG
                     KTHYVSVNVSPIQNGKKKINGIIIVFTDITDKYIAEQRLKDLLSEKEFLFRLFSENSI
                     DLIFRFDFLPKGRFTYLSPAVSAMTGYNPDEFYAYPEFVNKLIFPKDLSLWNSFISGE
                     IPSDSPTALRWIKKSGEIIWIELRCAMEYDKEGNIAGIEGIARDISRRKKSEDLLHRT
                     EEIYQHTFKYAPIGIANTSLNGRLLTVNRKFCDILGYRQEELLSMTFRDFTHPDFIST
                     SEELLEKGRTGETPNFIIEKKYIRKDGTVIWGRMSNLTVKNEDGSADYFIETLEDITE
                     QKKAEAEIQEREAKYRILLETMSEGAALNEIIYNE"
BASE COUNT         1193 a          797 c          828 g         1009 t
ORIGIN      
        1 tcagaggcac cttcggctca aaactggaag gctcttcagc ataagtgtag gattcggaat
       61 gaggaatcga agaaagaggg agacggtacc ccatcggtga atctcccgga atcagataca
      121 tcctgtccct tctgaaattt cttggacctg tttcccatac tgactcatag gaattccatc
      181 tgagaggaag accgaatcct gcaattttat cgagtccctg ctcaagaact tttctgagag
      241 tctttctttc ttctgaatcc ttcagatttt ttttgaaagg gtccacgttt acaggaagcg
      301 tcccctcctt ccagagataa taatacacat cctcatagcc cggaactaca tacctgtctg
      361 taatgttcag aaatgatgta agtgttttta tgaattcata tgcatgttta tgatccattc
      421 ccaggttcag attttcatct gcaatgagcg cctggtccct ccatacaggt ataccgtcct
      481 ttctccagat gcaggccaga gcccatcttg gaagagattc tccgggatac cattttccct
      541 gcccgaaatg cagaagtgga ccgaatcccc attttccctt cagacggtga agcagatcaa
      601 gagcatattt ctttttagta ggcccgagag cttcagtgtt ccattctgca ccgtccatat
      661 ccagtgcaga tacaaaagta ggttctccgc ccattgtcag acgaacgtcg gtctccttca
      721 tttcagaatc aatctttgca ccgagagaat atatcttacc ccattgtcct tcagtgtacg
      781 gtttggtggt tctgggtttc tcctgaatcc gggtaacgct catctcataa ttaaatttta
      841 catctgcttt ttccgtcgct ccggtaagag gagcagcgcc tgcaggttca ggagtgcatg
      901 ccagcggaat atgaccttcc cctgcaaaaa gtccggaagt agggtcaagc cccacccatc
      961 ctgctcctgg cagatagacc tcagtccatg catgaagatc tgtaaagtcc tcggaaggac
     1021 cggctggacc gtcgagaggc tttacatctg cagtaagctg aataagataa ccggaaacaa
     1081 aacgcgcggc aagtccaagc ttgcgaagag tctgcacaag gagccatgca ctgtccctgc
     1141 aggaaccttt cccgattttc agcgtttctt cgggagtctg aaccccgggt tccatacgga
     1201 taatataatc aacatgctta tggacgatct gattgatttc caccagaaaa tccactgatc
     1261 ttttttcgtc catcgggaca gaggctgcaa aatcattaaa cttggatgat tcttcattta
     1321 cataaagata gggaagaagt tcgagcttca gaccgtcatc atatttgaaa gggaaccttt
     1381 ctgcattcgg ctccagaaaa aaatcgaaag gattgatgac tgtcatatca gctgtaagat
     1441 cgacttcaaa ggaaagttct tttgattttt ccagaaaaac aaatcttgcc tgataatttc
     1501 cgaacggatc ctgctgccag ttcagaaagt gttttttagg caggacattc aaggaatagc
     1561 ttaaaactgg tgttctggta tgcacggccg gtctgagtct gattacgtga ggaaatatgc
     1621 tcacaggttt ttcatagatg tattctgttt tatggtgcag cttgactcgt atcgacataa
     1681 tggtttcccc cgacttctac atgcaagaaa tgcgccaaaa cagaagtctg ctgataatat
     1741 ttacaggatg gtcattctga aggatatagc agataaataa gactgatgaa ccagataatc
     1801 acctatttca tttaaaaaag atttcctaag ccagagacag attggaaaat actgatgcag
     1861 cttctatttt cgggagaaag cagagaagca tctgcttaaa atgcaggcat tttgttgaag
     1921 agaaagaaat gagattttgg aaacatcaaa aggattgagg agcacagatc tatggatttc
     1981 agtcgaatcc agtctattta gggtaaactt tcccgattat ttcgacctca atacgaaata
     2041 tcggccagtg tattcaggct aaaaaaggat tcaacagaaa acagacagga gagcagtcaa
     2101 cctgcgataa agtcagaact gaaaaaattt cagaacaatt aaaacatttg acaattgtcc
     2161 aacgtaaaag gttttagata gagcgggggc ttgtgccatg aaagattcaa aatctatcct
     2221 tttcattgaa gacagtgaac ttcctcagat tcgggaaata gagaatctga aaaaatccgg
     2281 ctatgatatc attccggtca agtcaggcga agaagctctc aggtcagctt cggcaaacgg
     2341 caccgtcaat ctggtgatga tggatatgag agaagaccga aaggatcttg ttcaaaaaat
     2401 tctgtcggaa agaaatcttc ccattctttt cgtcgtatcg gattctgata tagaatatat
     2461 agacaaagtc agagaatttc ctaactgcac atatatgatg aagaattctg atgaattcac
     2521 gctgaagtct tcggtagatg ccgcatttga actttttgat gcgaacgagc tcaaaaaaga
     2581 gaatgaggaa tcgcttaagg tcactcttca gtcgataggc gacgctgtga tcacggcaga
     2641 tcttaaccga aaggtgagca ggatgaatgc ttctgcggaa gcgctgaccg gctggagttt
     2701 taaggaagca gaaaacaaac gtctggaaga tgtattcaag accgtaaata tagactcctt
     2761 tgacagcctg aaggaaactg tggaatcagt cattaaaaac ggaagaacgg caaggttttc
     2821 agaacatgca attctgactg acagacaggg caaaactcat tacgtcagcg tcaatgtatc
     2881 tcctattcaa aacgggaaaa agaaaataaa tggaattatc attgtattta ctgatattac
     2941 agataaatac attgctgaac agagactgaa agatctgctt tcagaaaagg aatttctatt
     3001 ccggctgttt tcagaaaact caatagatct gatcttcaga ttcgactttc tccctaaagg
     3061 cagattcacg tatctgagtc cggcagtttc tgcaatgaca gggtacaatc ccgatgagtt
     3121 ctatgcatat cctgaattcg taaataaact tatatttccg aaagacctgt cactctggaa
     3181 ttcatttatt tcaggtgaaa ttccttccga ttcgccgaca gctctcagat ggataaaaaa
     3241 gagcggggaa atcatctgga ttgaactgag atgtgcaatg gaatatgaca aggaaggaaa
     3301 catcgccgga attgaaggta ttgccagaga catttccaga agaaaaaaat cggaggatct
     3361 gcttcacagg acagaagaaa tctatcagca tacattcaaa tatgctccta ttggaattgc
     3421 aaatacttca ctgaacggac gtcttcttac agtgaacaga aaattctgcg atattttggg
     3481 ataccgtcag gaagaacttc tttccatgac ctttagagac ttcactcatc ctgatttcat
     3541 cagcacatct gaggagcttc tcgaaaaggg acgcaccgga gagacaccaa attttatcat
     3601 tgaaaaaaaa tacatccgca aggacggaac agtgatctgg ggaaggatgt cgaatctgac
     3661 tgtaaaaaat gaagacggtt ctgccgacta tttcattgaa actcttgaag acattacaga
     3721 acagaaaaaa gcagaagcag aaattcagga acgagaagca aaatacagaa tcctgctgga
     3781 aacgatgagc gagggcgctg ctctgaatga aatcatctac aatgaaa
//
LOCUS       DAOQGJ010000116         3818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13370_length_3818_cov_2.652139, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000116 DAOQGJ010000000
VERSION     DAOQGJ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13370_length_3818_cov_2.652139"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK683_02780"
     CDS             <1..538
                     /locus_tag="PK683_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH07401.1"
                     /translation="HPFFHELPDFILVQNQEIPAHHPIHSALILISSEMDSGGSSELI
                     LHRLSDILLYYILKYWADRNSSAEPGWIRTMKDEKILYVLDLLHRDISRDWTLDSISD
                     SVGISRASLANKFRESLGITPLDYLSRVRIEKGRRLLQEKNYTLEEIARKVGYSNAFS
                     FSKAYKRVRGVSPKTETA"
     gene            618..2036
                     /locus_tag="PK683_02785"
                     /pseudo
     CDS             618..2036
                     /locus_tag="PK683_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            2033..2437
                     /locus_tag="PK683_02790"
     CDS             2033..2437
                     /locus_tag="PK683_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07402.1"
                     /translation="MIQGFIINSILCSVIFIIFFLGYPFRNEMKIQYAYLFLLPAVLL
                     GILQGYLAGGMWRSRFQKLFLLAVFLVYLFNIGQSAILYFPKAGNAFLTLDTWKAIMI
                     GANTNFILFGFITLPYLLISTFLIEIWVEPTR"
     gene            complement(2438..3193)
                     /gene="cobS"
                     /locus_tag="PK683_02795"
     CDS             complement(2438..3193)
                     /gene="cobS"
                     /locus_tag="PK683_02795"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNH07403.1"
                     /translation="MKFILLQWNLFCTSVMFLSRIPLYPTEFREEYLNESAGYFPLTG
                     MLIGFLSGSVYYGASFFFSTSVSVILSMCFSVLITGAFHEDGFTDFCDGFGGGYGDRE
                     KIIQIMKDSRIGAFGTVGISLLLILKWQLLSELPKDRIFLSIISAHSLSRAVLLYFLR
                     TDEYAAENGKSKPMAKKISYSGLLFGTLISAVPLFLFRNEKIFLCIIPLTVFKILYSS
                     YLRKRLGGYTGDCLGAAQQIAEVLLYMSLLKLL"
     gene            complement(3190..>3818)
                     /locus_tag="PK683_02800"
     CDS             complement(3190..>3818)
                     /locus_tag="PK683_02800"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNH07404.1"
                     /translation="PAMTLDELYPALSIGRELVRTEIESGCNTFLFGEMGIGNTSSAS
                     LLTSIYLGMEITECTGRGTGLNDLQLIHKLKVLEECRNRFSEGTPVQVFAEFGGYETA
                     MIAGGMLEAASQSKLIVVDGFIVTSSLLFVRELYPEIMNFCIFSHVSDEYAHKSVLSR
                     IGAVPLLDLSMRLGEGSGAAAALPLLRNALALLRDMASFDSAGVSGKK"
BASE COUNT         1144 a          855 c          808 g         1011 t
ORIGIN      
        1 gcatcctttc tttcatgaac tccctgactt catccttgtg cagaatcagg aaattccagc
       61 ccatcacccg attcactccg ctctgattct catttccagc gaaatggatt ccggaggaag
      121 ttcagagctg atactccaca gactgtctga tattttatta tattatattt taaaatactg
      181 ggcggacaga aacagctctg cggaaccggg ctggatcagg acaatgaagg atgagaagat
      241 tctgtatgtg ctggatcttc ttcacagaga tatttcacgc gactggacac tcgattccat
      301 ctcggactct gtcggaattt cgagagcatc cctggcgaac aagttcaggg aatctctcgg
      361 aatcacccct ctcgattatc tctcccgggt cagaattgag aagggaagaa gacttcttca
      421 ggaaaaaaat tacacactcg aagaaattgc aagaaaagtc gggtattcaa atgcattttc
      481 cttttccaag gcatacaaaa gggtcagagg ggtttctccg aagacggaaa ctgcctgatc
      541 tgaaccgaat ttcacaggtt tttaatggtt tacaatttcc ctttgcacag gcgatatgga
      601 cttgatttat gaaaacaatt gaacttccca aaccagagaa agtcctttcg tggggaatga
      661 cccatgaaac agagagtccc gttctccgtc ccgcttcaga agaggacatc gcagaaattt
      721 tcaaggctgc aaatgagaaa aactggaagc ttactttcag aggaggcgga tgcagctacg
      781 gtgatgcttc tctaaatgga aaaggcgtca ttgtagatat ctcaggctac agccggatta
      841 ctggctggga cgcacagaca ggcacaatca aggcacagtc gggtgtcaca atcaagcagc
      901 tttgggagtt tgccatcgaa aaaggttttt ggcctccggt agtcagcgga acaatgatgc
      961 cgactctcgg cggcgcactc tccatgaaca ttcacgggaa gaacaatttt gttcagggtc
     1021 ccatcggaga ccatgtaatt gaatttacat ttctgacccc taaaggagac agactgacct
     1081 gcacaaggaa ggacaacaag gaactcttct actctgcaat cagcggaatc ggaatgctcg
     1141 gatgtttcct ggaagtaaca ttaaaactga aaaaaatcta ttcagggaaa atggccgtca
     1201 ctccggttcc tacaaagaat ctggaagaga tgatggacta ttttgaaaaa aattattcca
     1261 gctcggatta tcttgtagga tggattgatg cattcggatc aggcagttca ttagggagag
     1321 gacagattca taaggctaat tatattccag aaggacagga ccctgattat tccgaaaata
     1381 tcaaactgaa gaatcagcat cttccttcca gactgtttta tctgattcca aaatcctgga
     1441 tgtgggtttt aatgtctcct ttttccaaca agctgggaat gagactgatc aattatgcaa
     1501 agtacactgc cagcagactt ctgaccaagc cttataagca gggacatgct gaatatgcat
     1561 ttcttcttga ctacgtccct aactggaaat ttatttataa acctggaagc atgattcagt
     1621 accagacatt tcttccgaaa gaaaccgctc tgaatgccta caaggaaatc ctcacccact
     1681 gtcagaagag aggaattgta tcctttcttg ccgtattcaa aaaacacaga aaagatgact
     1741 ttcttcttac tcacagtgta gacggatttt ctctggcatt ggactttcct gtcagtccta
     1801 aaacgaagga tgccgtatgg aaaatggcat atgaactcga tgagattgta ctgaagcatt
     1861 caggaagatt ttattttgca aaggattcta ctctgcgtcc tgaaatcgct gaaaaagtct
     1921 tcggaaaaga gactattgat aaattcaaaa aaatcaagaa tgaactcgat ccaaagaata
     1981 tactggaatc cgatctttac agaagagttt tcgcgagcgg aggaagcagc aaatgataca
     2041 gggatttatt atcaattcca ttctgtgttc cgtcattttc attattttct ttctgggata
     2101 tcctttccgc aacgaaatga aaattcagta cgcttatctt tttctgctcc ctgcagttct
     2161 tctcggaatt ctgcagggtt atctggcagg aggaatgtgg agaagcagat ttcagaaact
     2221 gtttctgctg gcagtgtttc tggtgtatct cttcaatatc ggacagtctg caatccttta
     2281 ttttccgaaa gcaggaaatg catttttgac actggatacc tggaaagcaa ttatgatagg
     2341 tgcgaataca aacttcatac tgttcggatt catcaccttg ccgtatctcc tgatcagcac
     2401 ttttctgatt gaaatctggg tagagccgac cagataatca gagaagtttc agcagactca
     2461 tataaagtag cacctctgcg atctgctgag cagctcccag acagtcccct gtgtatcctc
     2521 ccagtctttt ccgtagatag gaggagtaga gaatcttgaa tacagtcaaa gggatgatac
     2581 ataaaaatat tttttcatta cggaaaagaa aaagaggaac ggcagaaatc aacgtgccga
     2641 aaagaagtcc tgaataggaa atcttctttg ccatcggctt ggattttccg ttctcggctg
     2701 catactcatc tgtacggaga aaataaagaa gaactgctct gctgagagaa tgtgcactta
     2761 tgatagacag aaatatccta tccttaggca gttcagaaag aagctgccat ttcagaatca
     2821 gaagaagaga tattcccacc gttccaaaag ctcctatcct ggaatccttc ataatctgaa
     2881 tgatcttctc cctgtctccg tatcctcctc cgaacccgtc gcagaaatca gtgaacccgt
     2941 cctcatgaaa cgcacctgta atcagcacgg aaaagcacat cgaaagaatc actgagaccg
     3001 acgtcgaaaa aaagaaactt gctccgtaat aaactgaacc tgaaagaaat ccgatcagca
     3061 ttcctgtgag aggaaagtat ccggctgatt cattcagata ctcttctctg aattccgtcg
     3121 gatatagagg gattctgctc aggaacatga ctgaagtgca gaacaggttc cattgaagca
     3181 ggataaattt catttttttc cggatactcc tgccgaatca aatgaagcca tatcacgcag
     3241 gagtgccagt gcatttctca gaagaggaag agccgccgcc gctcccgacc cctctccaag
     3301 ccgcatcgaa agatcaagca gaggaactgc accgattctg ctgagaaccg acttgtgggc
     3361 atattcatcg gaaacatgag aaaatataca aaaattcatt atttcaggat aaagttccct
     3421 gacaaaaagc agagaagaag tcacaatgaa tccgtccacg acaatgagtt tcgactgaga
     3481 ggccgcctcc agcattcctc ctgcaatcat tgcagtctca tatcctccaa actctgcaaa
     3541 aacctgtaca ggagttcctt cgctgaatct gtttctgcat tcttcgagaa ccttcagctt
     3601 gtgaatcagc tgaagatcat tcagtcctgt tcccctgcct gtgcattcag tgatttccat
     3661 tccaagatat atcgaggtca gaagagacgc tgaagatgta tttcctattc ccatttcacc
     3721 gaacagaaat gtattgcatc ctgattcgat ttcagtcctg acaagttctc tcccgatgct
     3781 cagtgcagga taaagctcat ccagagtcat tgcaggtt
//
LOCUS       DAOQGJ010000117         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13409_length_3810_cov_3.603196, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000117 DAOQGJ010000000
VERSION     DAOQGJ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13409_length_3810_cov_3.603196"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..1985)
                     /locus_tag="PK683_02805"
     CDS             complement(285..1985)
                     /locus_tag="PK683_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNH07405.1"
                     /translation="MLLLNPKKPDFSKLDEKSKNIMLKTIEFFEKKGKKKLKHDDHER
                     VWYADFLESQKEIGAFATLMTPKGYGPSDSRFDSHRNCHYSEVLGFYGLCYWYTWQVS
                     MLGLSPIWMSKNEKIKNRTAELLKNGEIFAFGLSEKEHGADLISTSMTLTPEGDGKYK
                     ANGSKYYIGNGNKAAIVSTFGKIGDSKSFTFFALNSQHPNYKLIKNVTNTQNYVSAFD
                     VKDYPVSEEDILSRDRDAWDTSLATVALCKYNLGWATIGIVTHAFYEAIAHSANRRLY
                     GQYVTDFPHIKQLFMDGYARLVAMRLFTMRASDYMRSANEKDRRYLLYNPMVKAKVTM
                     QGEEALNCFWDAIAAKGFENDMYFETAARDIRGLPKLEGTVHVNMALINMFMDSMLFS
                     PAEMPVIPERTDLADDTYMFNQGPTTKGLKKVQFHDYKKAYSLYKSENISIFLEQVEG
                     LKHFLKDSPPSAEQSRDLDFMLSLGEIFTLVPYGQLILERADQLKVDKDVIDQIFDVM
                     IRDLSRYAVQLHTKPSSTEAQMKNAIAMVRKPKTDNERYERVWTTYVHSQKDAYQMNP
                     "
     gene            complement(2440..2895)
                     /locus_tag="PK683_02810"
     CDS             complement(2440..2895)
                     /locus_tag="PK683_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH07406.1"
                     /translation="MSSVRMQRITPFLWFDSNAEEAVRFYTSVFENSKIIQISPMVST
                     FELDGQRLMALNGGPHFKFNEAVSFYVNCETQKEVDYYWEKLLSGGGKESQCGWLKDR
                     FGLSWQIVPTALGELMSDPDPVKSQRVLQAMLKMIKLDVEGLKKAHRGE"
     gene            complement(2892..3314)
                     /locus_tag="PK683_02815"
     CDS             complement(2892..3314)
                     /locus_tag="PK683_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH07407.1"
                     /translation="MNDKIMITVSAVIKAPAEKVWKLWSTPEDIMNWNSASPDWHTPA
                     STNDLRKGGKFSSTMAARDGSMSFDFWGIYDEVKENELIAYTMGDGRKVTVTFRQNGN
                     ETEVTEIFEAEGVHPPEMQKAGWQSILDNFKKYAEAKV"
     gene            complement(3514..>3810)
                     /locus_tag="PK683_02820"
     CDS             complement(3514..>3810)
                     /locus_tag="PK683_02820"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH07408.1"
                     /translation="PLADADKNLIMHIELPILGGHVIMGTDAPESMGFKLNQGNNVYI
                     SLEPDTKAETKKLFDALSKGGTVEMELQDMFWGAYYGSCTDRFGIRWMFNCSTK"
BASE COUNT          967 a          864 c          800 g         1179 t
ORIGIN      
        1 cagctctttt gaagggatgc tgttatatca ttgaacttaa agtaaatgaa atgactcctg
       61 aaggaaaagc aatcactcag atcaaagaaa gaaaatacca ccagaaatac atcggttatg
      121 gaaagctctt gcataagtac cggcagtatt aaagagattt atatcattgg tgcagaattc
      181 agcaaggcag agagatacat cacaggcttc gactgggaaa ggatcttatg actgactgta
      241 tttagacctc gaggttttca taaacctcga ggttttcaga tgaatcaggg attcatctgg
      301 taggcatctt tctgggaatg aacataagtc gtccagactc tttcgtatct ttcattgtct
      361 gttttcggct tgcgcaccat tgcaattgca tttttcatct gcgcttcagt agaagaaggt
      421 ttggtatgaa gctgaactgc ataacgggac agatcgcgga tcataacatc gaatatctga
      481 tcaatcacat ccttgtccac tttcagctga tctgctcttt ccaggatgag ctgaccgtaa
      541 gggacaagtg tgaatatttc ccctaaagaa agcataaagt ccagatctct tgactgttct
      601 gcactcggag gagagtcttt caggaaatgc ttcagtcctt ccacctgttc gaggaagatg
      661 gaaatatttt cagacttgta aagactgtaa gcttttttat aatcatggaa ctggactttc
      721 ttcagaccct tggtagtcgg tccctgattg aacatatagg tgtcgtctgc gagatcggtt
      781 ctttcaggaa tcacaggcat ttccgcaggg ctgaaaagca tactgtccat gaacatattg
      841 atgagagcca tattcacatg cacagttcct tccagtttcg gaagtccgcg aatgtctctg
      901 gctgcagttt cgaaatacat atcgttttca aaacccttcg cagcaattgc atcccagaaa
      961 cagttcagag cctcttcccc ctgcattgtg acctttgcct tcaccatcgg attgtacaga
     1021 agataacgtc tgtctttctc gtttgcactt ctcatataat cggaggctct cattgtaaag
     1081 agtctcatcg cgacaagcct tgcatatccg tccatgaaca gctgcttgat atgagggaag
     1141 tctgtcacat actgaccgta cagtcttctg ttggctgaat gagcgatcgc ctcatagaat
     1201 gcatgagtga cgattccgat tgtagcccat cccagattgt acttgcagag agcaactgtt
     1261 gcaagagagg tatcccatgc atctctgtct ctggaaagaa tatcctcttc agaaacggga
     1321 tagtccttta catcgaatgc ggatacatag ttctgagtgt ttgtaacatt tttaatgagt
     1381 ttataattag gatgctggct gttcagagca aagaaagtga aactcttgct gtctccgatt
     1441 ttgccgaatg tagatacgat cgcagccttg tttccgttgc cgatataata tttgcttccg
     1501 ttcgccttgt actttccgtc tccttccgga gtcagagtca tgcttgtgga aatcagatcc
     1561 gcaccgtgtt ctttttcaga aaggccgaat gcgaaaattt ctccgttctt cagaagctct
     1621 gcagttctgt tcttgatttt ttcattcttg ctcatccaga tcggagagag gccgagcatt
     1681 gacacctgcc aggtgtacca gtagcagaga ccgtaaaatc cgagaacctc actgtaatgg
     1741 cagttcctgt gactgtcgaa tctggaatca gaaggaccgt atcctttcgg agtcatcagc
     1801 gttgcaaacg cgccgatttc tttctgagat tccaggaagt ctgcatacca gactctttca
     1861 tgatcatcgt gtttcagctt tttcttgcct tttttttcaa aaaactcgat cgtcttcagc
     1921 ataatatttt tggatttctc atccagtttg ctgaaatcag gcttttttgg attcaacagg
     1981 agcatttcta cctctcgttc tgcgacggtt tttattggga gaaccccgcc gtcttgtctt
     2041 tagattagat tttcagacgg aacgatgcag gcaagcaaaa atgattgaga gcttcggtga
     2101 aacatcctgt tctcagtttt tgaaatatcg tctgattccg actgtaaaaa aagcagaact
     2161 gatattggaa tggaataggg ccgaaagaat cggaacagta cagggaatac gtcgagaaaa
     2221 aagctgagag cggcagtgag ggaaatatct ccgttttcat cctgcagatc catccatgct
     2281 gccaaggccg gcagtgcatg atgcaatcat taaatttata agaatgctta ggatagatat
     2341 cctaaacacg gatcttgtga aaaccttcat ttaagaattc ttgctccccg gttgaagaca
     2401 tcagactggt tttaactcca gcctgccatc ctgtgttttt tattcccccc gatgcgcttt
     2461 tttcagtcct tctacgtcga gtttgatcat cttcagcatt gcctgaagga ctctttgtga
     2521 tttgaccgga tcggggtcgc tcatcagttc gccgagtgct gtcggtacaa tctgccagga
     2581 aagaccgaat ctgtctttca gccatccgca ctgagattct tttccgcctc cggaaagaag
     2641 cttttcccag taatagtcca cttccttctg agtttcgcag tttacataaa aggaaacagc
     2701 ctcattgaat ttgaaatgag gtcctccgtt gagtgccatc agtctctgtc cgtccagctc
     2761 aaatgtggat accataggac ttatctgaat gatctttgaa ttctcaaata cagatgtgta
     2821 gaatctcact gcttcttctg cattgctgtc gaaccaaaga aatggagtga ttctctgcat
     2881 tctgacggaa ctcatacttt ggcctctgca tattttttga aattgtccaa aatagactgc
     2941 cagccagctt tctgcatttc aggaggatgt actccttccg cctcaaaaat ttctgtcacc
     3001 tctgtctcat ttccattctg tctgaatgtc acagtcactt ttcttccgtc acccatagta
     3061 tatgcgatga gttcattttc tttgacctca tcatagattc cccagaaatc aaacgacatg
     3121 cttccgtctc tggctgccat tgtggaggaa aactttcccc cctttctcag gtcatttgtt
     3181 gaagcaggag tgtgccagtc gggtgaggca ctgttccagt tcataatgtc ttccggggtt
     3241 gaccagagtt tccatacttt ttctgcagga gcttttatca ctgcacttac agtaatcatt
     3301 attttatcat tcattgtatt gtcctttaac tgctcagcct ggcggcttcg cagttacaaa
     3361 tatccttttt ctagagttta cttttaaaaa agcaagaaaa ggtttatccc tatcgggagg
     3421 atttaggggt ttgcagcccc taagaccccg cttatcagct tttctatata agtttactta
     3481 cagtattttt tcacagagta ggtactgtgt cctttatttc gttgagcagt tgaacatcca
     3541 gcggatgccg aatctgtcag tgcagcttcc gtaataggct ccccagaaca tatcctgaag
     3601 ttccatttcg actgttccgc cttttgagag tgcatcaaaa agtttttttg tctctgcctt
     3661 tgtgtccggc tcgagactga tgtacacatt gtttccctga ttcagcttga atcccatgga
     3721 ttcaggagca tcggttccca taatgacgtg tcctcccaga ataggaagtt caatatgcat
     3781 aatcagattc ttgtctgcat cagcaagcgg
//
LOCUS       DAOQGJ010000118         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13452_length_3803_cov_3.219851, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000118 DAOQGJ010000000
VERSION     DAOQGJ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13452_length_3803_cov_3.219851"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PK683_02825"
     CDS             <1..724
                     /locus_tag="PK683_02825"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNH07409.1"
                     /translation="NDVLLLGEEHDDEDGHRLKLRLMEYFAEREKFGIGMEFLERDSQ
                     VPADEYLSDFIDEKLLLSSLSVSVSGQINPYLPFIRLAKMKNLPLIATNVQRRYARIV
                     SQKGLTELKKLKTLLKSDLPDLEEVNSVRSPVYDSKLSALFGSHSGAENERTERFVEA
                     QNLWDASMCEKIHSFVTEKKQKLIHINGRFHSDDHLGLAFRLKKKGLRVITVSMIRTE
                     TEETARPDYFPLADIVWITGKR"
     gene            complement(725..2068)
                     /locus_tag="PK683_02830"
     CDS             complement(725..2068)
                     /locus_tag="PK683_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNH07410.1"
                     /translation="MKIYIADDELEIRRALRQVLEDEDYEVEDFSNRKSLLKSLSRER
                     PALILLDVWMGKDDGLEILDECRKIYPLIPIVMISGHGTIEMAVNATKKGAVDFLEKP
                     LSIEKVLGTIENVLQQNKSEALPEVRLEYDPIIGNSSSIRKVKFAIAQAAAANVRVFI
                     YGENGTGKELVAKTIVMNSRRKTNPYIEINCAAIPEELIESELFGHEKGAFTGAHDRK
                     IGKFEAANSGTLFLDEICDMSLSAQAKVLRILQEQRFERVGGTETMQVDVRVIAATNI
                     PVEDAIKEGRFREDLYYRLNVIPIVIPPLRERRTDIPLLLDYYMKETAAENQLSSKKI
                     DKEGVEILTNHFWPGNIRELKNVVERLCIMTVSDTIRAKDAKDALKGFSKAEEIFEQG
                     DLKKAKEEFEREYIIKTLQNNEGNISRCAKILGIERTNLYRKMKSLNIQIDNLID"
     gene            complement(2071..>3803)
                     /locus_tag="PK683_02835"
     CDS             complement(2071..>3803)
                     /locus_tag="PK683_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07411.1"
                     /translation="CIFVAIALAETIIFNDKSNADFTDRIIGYGFFVIPFLILALIVL
                     SIYRNFRIRKTGKARSSIRYRLTIGFIFVSILPAIPVVILSSNVIGKLVESFYRIDVS
                     AALRHSRNFVAYTESEDRKHIYQKARLLHSYLGNRDPSPEFIQKNLNDLGISDADTYY
                     AAYFSRNVLIAEVPYIGKVFPENFTKKDDEIFSSFNLYRKDKAYIIFRINLHREGNFI
                     LLGKKIHAGMEDAIFDILNTENSYNVADLWKEKVPFSLRLTLGIFFIAIFAISIAVSF
                     LFARQISRPIVLLADAVRLVSKGETDVILENREEGEMGILIESFNQMTMDLKSKTEEL
                     YTIQRVAAWKEVAQRMAHEIKNPLTPIQLSAERIRRNLENPNREKFSEIVRSGTDTII
                     GQVRVLEHLVKEFSEFARMPSPVLINQNIEPIMEESVQLFRDSHPEIEFRLKVSKNLP
                     EVFIDKRLFLGAVNNLIKNAVEAVMTQKKEETAEEKKTDVISVSASVERKFIRKHVII
                     SVEDSGPGIPEKMREKIFEAYFSTKEGHGSGIGLTIVQKTILDHHGHIYVESSKLGGC
                     CFKIELPCQE"
BASE COUNT         1003 a          883 c          739 g         1178 t
ORIGIN      
        1 caatgatgtt ctgctcctgg gagaagaaca tgatgacgag gacggccaca gactgaaact
       61 caggctgatg gagtattttg ccgagcggga aaaattcgga ataggaatgg aattcctcga
      121 aagggattca caggtgcctg ccgatgaata cctgtccgac tttatcgatg aaaaactgct
      181 tctgtcatca ttgagtgtca gtgtctcagg ccagataaat ccgtatcttc ctttcatccg
      241 cctggcaaaa atgaaaaatc ttcctctgat tgcaacaaat gtgcagaggc gttatgcaag
      301 aattgtatct cagaaaggtc ttactgaact gaagaagctg aagactcttc ttaagtccga
      361 ccttcctgat ctcgaagaag tcaattcagt ccgcagtcca gtgtacgatt caaaactgtc
      421 cgcactgttc ggatcacatt ctggggccga aaatgaaaga acggagagat tcgtggaggc
      481 acagaattta tgggatgctt ccatgtgcga aaaaattcat tcctttgtga cagagaaaaa
      541 gcagaaactc attcatatca atggaagatt tcacagcgat gatcatcttg gactggcttt
      601 ccgtctgaaa aaaaagggac tgcgtgtaat tacagtctca atgatcagga cagaaacaga
      661 agagaccgca agaccggatt attttcctct tgcagacata gtatggatca caggaaaacg
      721 atagtcagtc tatcagattg tcaatctgta tattaagaga tttcatcttg cggtacagat
      781 tggtcctttc tattcccaga atttttgcgc atctggaaat gttgccttca ttattctgca
      841 gcgtttttat gatatattct ctttcaaact cctctttggc cttcttgaga tctccctgtt
      901 caaagatctc ttccgccttg ctgaaccctt tcagtgcatc cttcgcatcc tttgcacgga
      961 ttgtatctga tactgtcata atgcagagtc tttcaacgac attcttcagc tcacggatat
     1021 ttccaggcca gaaatggttt gtaagtatct ccactccttc cttgtctatc ttctttgagg
     1081 aaagctgatt ttccgcagca gtctccttca tataatagtc cagaaggaga ggtatatccg
     1141 ttcttctctc tctgagggga ggtattacaa tcggtattac attcagtctg tagtaaagat
     1201 cttctctgaa acgtccttcc ttgattgcat cttccaccgg aatgtttgta gctgcaatga
     1261 ctctcacatc cacctgcatc gtctctgtgc cgcctactct ttcaaatctc tgttcctgca
     1321 gaattcgaag aaccttcgcc tgagctgaca gcgacatatc acagatctca tccagaaaaa
     1381 gagttcctga atttgcagcc tcaaacttgc ctattttcct gtcatgagct cctgtaaaag
     1441 cgcccttctc atgtccgaaa agctccgact caatcagctc ttcaggaatg gcggcacagt
     1501 tgatttctat gtacggatta gtttttcttc tggaattcat tacaattgtc tttgcgacaa
     1561 gttcctttcc cgttccgttc tccccatata taaaaactct tacatttgca gccgcagcct
     1621 gtgcaattgc aaacttgact ttccttattg atgaagaatt tcctatgata ggatcatatt
     1681 ccagccttac ttcaggaaga gcctctgact tgttctgctg aagaacattt tcaatggttc
     1741 ccaggacctt ctctatggaa agaggttttt ccagaaaatc aactgcacct tttttggtgg
     1801 cattgactgc catctcgatt gtcccgtgtc ctgaaatcat gacgatggga atcaggggat
     1861 aaatctttct gcactcatcc agaatttcga gaccgtcatc ctttcccatc cacacatcca
     1921 gaaggatcag tgcaggtctt tcccgggaca gacttttcag aagcgacttc ctgtttgaaa
     1981 aatcctccac ctcatagtct tcatcttcaa gcacctgccg aagagctctc cgtatttcca
     2041 attcatcatc tgctatatat attttcatga ttactcctga cagggaagtt ctatcttgaa
     2101 gcagcatccg ccgagcttag aactttccac ataaatatga ccgtgatgat ccagtattgt
     2161 cttctgcaca atagtaagac cgatcccgga gccgtgtccc tccttggtgg aaaaataagc
     2221 ctcaaatatt ttttctctca ttttttcagg aatccccgga ccgctgtctt ccacactgat
     2281 tatgacatgt tttctgatga actttctttc cactgaggca ctgactgaaa tcacgtcagt
     2341 ttttttctct tccgctgtct cttctttttt ctgagtcatc actgcctcta ctgcattttt
     2401 gatcagattg ttcactgcgc ccaggaaaag ccttttgtcg atgaacactt ccggaagatt
     2461 tttggatacc ttcagtctga attctatttc aggatgactg tctcggaaaa gctgtaccga
     2521 ttcttccata ataggctcaa tattctgatt gatgagaacg ggagaaggca ttctggcaaa
     2581 ttctgagaat tccttcacca ggtgttcgag cactctcacc tgaccgataa ttgtatccgt
     2641 gccgcttctt acaatttcgg aaaatttttc cctgttcgga ttttccagat ttctcctgat
     2701 tctttccgca gaaagctgaa taggagtgag aggattcttg atttcatgtg ccattctctg
     2761 agccacttcc ttccatgctg caactctctg tatcgtatac agctcttccg tctttgactt
     2821 cagatccata gtcatctgat tgaatgactc aatgaggatg cccatttccc cctcttcccg
     2881 gttttccaga atgacatcag tctcgccctt ggacacaagt ctgacagcat ccgcaagcag
     2941 gacaatgggt cttgaaatct gccttgcaaa aagaaaggat actgcaatag aaattgcaaa
     3001 tattgcaata aaaaatatcc caagtgtaag cctgagagaa aacggcactt tttccttcca
     3061 gagatctgcc acattgtatg aattttcagt attcagtatg tcaaagatcg cgtcttccat
     3121 tccggcgtga attttctttc cgagcagaat gaaattccct tctctgtgaa gattgattct
     3181 gaatatgatg tatgccttgt cctttctgta aagattgaag gatgaaaaaa tttcatcatc
     3241 ctttttagta aaattttcag gaaatacctt tcctatgtaa ggcacctctg caatcagtac
     3301 atttctgctg aaataggctg catagtatgt gtctgcatca ctgattccga gatcattcag
     3361 atttttctgt atgaactcgg gagatgggtc cctgttaccc agataactgt gaagcagtct
     3421 tgccttctga tagatatgct ttctgtcctc ggattcagta tatgccacaa aatttctcga
     3481 atgcctgagt gctgccgata catcaatcct gtaaaaactc tccacaagtt ttccgattac
     3541 atttgagctt agaataacga ccggtattgc aggaaggatg gatacaaata tgaatccgat
     3601 agtcagtctg taacggatgc tgctccttgc ttttccggtc tttctgatcc tgaagtttct
     3661 gtaaatggaa agtacaatca gtgcaagaat cagaaaaggt attacgaaaa atccgtaacc
     3721 gatgattctg tcagtaaagt cggcgtttga cttgtcatta aaaatgattg tttcggcaag
     3781 tgcaatcgct acaaatatgc aga
//
LOCUS       DAOQGJ010000119         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13513_length_3793_cov_2.036918, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000119 DAOQGJ010000000
VERSION     DAOQGJ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13513_length_3793_cov_2.036918"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="PK683_02840"
     CDS             <1..1372
                     /locus_tag="PK683_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH07412.1"
                     /translation="RHMGGLGLPFTVQSIAMELSARADAAFSIAVGCFNLAETIEKYA
                     SKEMVEKYVPLLAEGKLCAAMALTEPNYGSDLPNVQTKAVKNENGQWTITGTKRFITH
                     GCGFGDIPSVILTLARTGTPTSGARGLSFFLVHSKDVHIASIEKKLGLHCSPTCEVVY
                     ENSPAELIGEEGMGLVKYSMGMMNTARLSIAAQSLGIATAAYFEGKKYASERVQFGKL
                     IQEIPAVKKMLQTMERELAAMRCLLYEAGRSVDLYLWRSEHLKHSGTSEKEIKRDEQI
                     SLWEKLANLFTPMSKYYISETANWMASNALQIHGGSGYTEDYDVAKIYRDARITNIYE
                     GTTQLQIVACIGGIVAGLTPKGNLRTYIDLELGKFKPSQRLMELKNSLEKIYTVYSEK
                     KESSYRDTYAFEMVESATRFINGLLLERSASRLSDEEKNSRLKMADDYNKDSLAILAA
                     NLVRVS"
     gene            complement(1407..1748)
                     /locus_tag="PK683_02845"
     CDS             complement(1407..1748)
                     /locus_tag="PK683_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNH07413.1"
                     /translation="MKKVVFTEEAEQDLRDAVSWYESQKKGIGKNLANEVIEKVQKLS
                     LKPDSYSADHTGLRKTSLKSFPYIIYFLVKLPVVLILAVWHKSRLKAEVDKRAGSLNP
                     SENEGSQQRQV"
     gene            complement(1745..2014)
                     /locus_tag="PK683_02850"
     CDS             complement(1745..2014)
                     /locus_tag="PK683_02850"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HNH07414.1"
                     /translation="MGTSKLEIDYKELFFKISSFPEETAIQLLEYIDFLWEKHAPSEL
                     GLSETDQAELKKRVNKYIQNPETGITLDEVISNLEKDLGEKLKNK"
     gene            complement(2083..2979)
                     /locus_tag="PK683_02855"
     CDS             complement(2083..2979)
                     /locus_tag="PK683_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07415.1"
                     /translation="MRNSEPVCVMNGVSDFKLREYFEKQSNAELTSAPMWKLYRNSLV
                     THKFSENRLEKLAVFNSVINSVYQVHVFRGEKILFEFGIEIVSKSELKKCLSALPPAK
                     EKIRFVKEHTVKEIQFSQFAYADGENLILILVPSKAVSDWKDVHKYIQYIAGIYHYRK
                     SSKSNVLNIPALFEEHFSKQIEKITEEKPDLRMLAAVYRLEDLSSYCRIMGEQFTHGI
                     VKEINRTIRSFISEYDLIYTFSHSRYLVVTFNENNENAMIRLKNVLFRVQELIIRYNV
                     DLFEFEKDSLKNPEFYERFLVH"
     gene            3057..>3793
                     /locus_tag="PK683_02860"
     CDS             3057..>3793
                     /locus_tag="PK683_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNH07416.1"
                     /translation="MNRFLIFFAVFGTVLLTGYLYIAMRISQSFQISGIPKFALFAFL
                     GILAVLTPASYGLSFYEVNPAKEKTLVLIAFSSFGFFTIAVSLFAGFDILSWILSKTP
                     FLENTFSGTKELHQDYQISRKEMFDQFFRTSALVLSAGLAGYGLKKVSDGPKVYTVKI
                     PLEGLPDSLKGFRIVQISDVHIGPTLKKDFAKEITEKVNSLSPDMIAITGDLVDGSVE
                     RLRNEVIPFADLNSKYGTFFCTGNHEY"
BASE COUNT         1086 a          800 c          792 g         1115 t
ORIGIN      
        1 ccgtcatatg ggagggctgg gacttccttt cacggtgcag tctatcgcaa tggaactttc
       61 tgccagagcg gatgctgctt tttcgattgc agtaggctgt ttcaatctgg cagagacgat
      121 tgagaaatat gcttccaagg aaatggtgga gaaatatgtt cctcttcttg cagaaggaaa
      181 actctgtgct gcgatggcgc tcaccgaacc gaattacggt tccgatcttc cgaacgttca
      241 gacaaaagcg gtgaaaaatg aaaacggtca atggacgatc acaggaacaa aacgtttcat
      301 cactcacgga tgcggattcg gcgatattcc ttccgtcatt cttacacttg caagaaccgg
      361 cactcccaca agcggagcga gaggactttc cttttttctg gttcacagca aggatgtgca
      421 tattgcttcc attgaaaaaa aactgggact ccactgctcc cctacctgtg aagtggttta
      481 tgaaaacagt cctgcagaac tgatcggcga agaaggaatg ggactcgtca agtattcgat
      541 gggcatgatg aatacggcca gactctccat tgcagcacag tctctgggca tcgcaacagc
      601 agcgtacttt gaaggtaaaa aatatgcatc ggaaagagtg cagttcggaa agctgattca
      661 ggaaattcct gctgtaaaga aaatgcttca gacgatggaa agggaacttg ctgcaatgcg
      721 ctgtcttttg tatgaggcag gcagatcagt ggatctgtat ctctggagat cggaacatct
      781 gaaacattcc ggcacatccg aaaaagaaat caaaagagac gagcagatca gtctctggga
      841 gaaactggcg aatcttttca ctcctatgtc caagtactat atttcggaaa ctgcaaactg
      901 gatggcatca aacgctcttc agattcacgg gggaagcggg tataccgagg actatgatgt
      961 ggcgaaaatc tacagagatg caaggattac gaacatctat gaaggaacta ctcagctcca
     1021 gattgttgcc tgcatcgggg gaattgtagc aggactgact cctaagggaa atctcagaac
     1081 ctatatagat ctggaactcg gaaaattcaa accgtcccaa agacttatgg aactgaagaa
     1141 ttctctggaa aaaatatata cagtgtattc tgaaaagaag gagagctctt acagagacac
     1201 atatgcattt gaaatggtgg aatcagcaac tcgattcatc aacgggcttc tgctggaaag
     1261 aagcgcttcc cgtctttcag atgaggaaaa gaacagcaga ctgaaaatgg cagacgatta
     1321 caacaaggac agtctcgcaa ttcttgccgc caatctcgtc cgtgtaagct gattcaaaat
     1381 cggatcattt cctgtagaga cgggtctcag acctgtcttt gctgtgaacc ttcgttttca
     1441 cttgggttca aggaaccggc tcttttatct acctcagcct tcagcctgct tttatgccag
     1501 actgctaaaa ttaaaactac aggtaatttg actaaaaaat aaataatgta tggaaatgat
     1561 ttcaagctag ttttacgaag accggtatga tctgcagaat aggaatcagg ttttaatgac
     1621 aacttctgaa ctttctcgat tacttcatta gccagatttt ttccaattcc tttcttttga
     1681 ctttcatacc agctgactgc gtctcgtaaa tcctgttctg cctcttccgt aaatacaacc
     1741 ttcttcattt atttttaagc ttctctccca aatccttttc aaggttcgaa atcacctcat
     1801 ccaaagtaat tccagtttcg ggattttgaa tatatttatt tactcttttt ttcaattcag
     1861 cctgatctgt ttctgaaagc ccaagctcag atggagcgtg tttttcccaa agaaaatcaa
     1921 tatattctaa aagctgaatg gctgtttctt caggaaaaga ggatatctta aagaataatt
     1981 ctttataatc gatctctagt ttagatgttc ccataagata attcttgttt tctaaaaaaa
     2041 gaaagcaagc tttttatcag aaatctcgtc ggggcaaact tgtcaatgca caaggaatct
     2101 ttcgtaaaat tcaggattct tcagagaatc tttttcaaat tcaaacagat cgacgttgta
     2161 ccggataatg agttcctgca ctcggaaaag gacatttttc agccggatca ttgcattttc
     2221 attattttca ttgaatgtga ctacgagata cctggaatga gaaaatgtat aaatcagatc
     2281 gtattctgag ataaaactgc ggatagtcct gttgatttcc ttcacaatcc cgtgagtaaa
     2341 ctgctctccc ataatcctgc agtacgaact caaatcctcc agcctgtata cagcggcaag
     2401 cattctcaga tccggttttt cttctgtaat cttttcaatc tgcttagaga aatgttcttc
     2461 aaacagtgcg ggaatattca gcacattcga ttttgaagat ttcctgtaat ggtatatccc
     2521 tgctatatat tgtatatatt tatgcacatc tttccagtct gacaccgcct ttgaaggaac
     2581 cagaatcagt atcagattct ctccgtctgc ataggcaaac tggctgaact gaatctcttt
     2641 tacagtatgt tcctttacaa atcggatttt ttcctttgcg ggaggaaggg cggacaggca
     2701 ttttttcagt tcggacttgg aaacaatttc tattccaaat tcaaaaagga ttttctcacc
     2761 cctgaacaca tgcacctggt aaacggaatt gatgactgaa ttgaatacag ccagtttctc
     2821 cagtctgttt tctgaaaact tatgagtcac aagagaattt ctgtatagtt tccacatagg
     2881 cgcagatgtc agttctgcat ttgactgttt ctcgaaatat tccctgagtt taaaatcgct
     2941 gaccccgttc atcacgcata caggttcaga gtttctcaaa aaaaggtaaa gctgtttttc
     3001 tgaatttgaa taatttggga ttgtgaaggg aacgctctga aaataaatga tctggtatga
     3061 acagattcct gatctttttt gcagttttcg gcaccgttct cctgactggt tatttataca
     3121 ttgcaatgag gatcagccag tccttccaga tttccggcat tcctaaattc gcactcttcg
     3181 cttttctcgg aatcctcgca gttctgactc ctgcctctta cggcctttcc ttttatgagg
     3241 tgaaccctgc caaggaaaaa acccttgtcc tgatagcatt cagcagtttc ggttttttta
     3301 caattgcagt cagtcttttt gcgggcttcg acattctgag ctggatttta agtaaaactc
     3361 cttttttgga aaatacattc tcaggtacaa aggaactgca tcaggactat cagatttcca
     3421 gaaaggaaat gtttgatcag ttcttcagaa cctcagccct tgtattaagc gcaggtctgg
     3481 cagggtacgg tctgaagaaa gtctctgacg gtcccaaggt ctatactgta aagattcctc
     3541 tggaaggact gccggattca ttgaagggat tcaggattgt tcagatttcg gatgtccata
     3601 tcgggcctac tctgaaaaaa gactttgcaa aggagatcac tgagaaagtg aattcccttt
     3661 ccccggatat gattgcgatt acaggggatc ttgtggacgg aagcgtggaa cgtctcagaa
     3721 atgaagtgat tccgttcgct gacctcaatt caaaatacgg aacatttttc tgcacaggaa
     3781 atcatgagta ctt
//
LOCUS       DAOQGJ010000120         3788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13545_length_3788_cov_3.687383, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000120 DAOQGJ010000000
VERSION     DAOQGJ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13545_length_3788_cov_3.687383"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PK683_02865"
     CDS             <1..211
                     /locus_tag="PK683_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNH07417.1"
                     /translation="MHPEDKYAKRFDIDLLRCIFCGLCEEACPKGAIYLDGNGEMASD
                     NRNDLILTKERMTQKTGGPILGRRI"
     gene            complement(231..983)
                     /gene="map"
                     /locus_tag="PK683_02870"
     CDS             complement(231..983)
                     /gene="map"
                     /locus_tag="PK683_02870"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNH07418.1"
                     /translation="MIYIKNRTEIDKMRSACRFAAEILEYIAPYVKEGVSTIELNDLI
                     HEFTLKKGAKSAPLGYKGFPKSICTSVNDVVCHGIPRKEDVLKEGDIINIDVTPIVDG
                     YHGDTSKTFIIGKTLPEVEKLVQDTEAAMWIGIEQVRPGNRVDDIANAIDDFLTPKRY
                     GIVRDLMGHGIGRSFHEDPQVPHFRQVRKLAKLEPGMTFTIEPMVNLGGYQVYTDKKD
                     KWTVRTKDGKWSAQFEHTVLVTQSGVEVLTKL"
     gene            complement(989..2545)
                     /locus_tag="PK683_02875"
     CDS             complement(989..2545)
                     /locus_tag="PK683_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004759083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNH07419.1"
                     /translation="MMKERNLAAIDLGTNSFHIIVVRVKSDGTFESLTKEKESVRLGS
                     GSGGDDFITPEAMDRGIKCLKRFKGLADMHNAQIRAIATSALREAKNRQLFLERSEKE
                     VGLHIEVISGFEEARLIYFGVLQGLPVFDKKVFLIDIGGGSTELLVGSKGQVDYAQSF
                     KLGAIRLTDKFFFGDPDRDSEKKQCRFYVEGMIAPIRREIEKHSPEIVIGSSGTIQTI
                     ASMVIANRGEEPQRTLNNFSFTAEEFAKVSKILDDADTLKKRTKLPGLDAKRADIIVA
                     GSIILEEIFKNFSIKQLTVSDYALREGIIYDSIRKFEGQTNSEPYPLDNIRMKAVNSL
                     FNAFPYEKDHVDKVTQLSMKIFDDLKSFHKCTKEEREYLQAACMLHEVGFGISRSSHH
                     KHSYYIIRNSEFMVGFNFEEIEIIAQIARYHRKSPPKPKHMEFASLGASAQNTVRKLA
                     GILRIADGLDRSHQNIAEEISCEVQDKTVLFKIRCRKDVDPHIDIWSAQQKKEMFEDA
                     FNAKALFVTF"
     gene            complement(2558..3787)
                     /locus_tag="PK683_02880"
     CDS             complement(2558..3787)
                     /locus_tag="PK683_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH07420.1"
                     /translation="MSGAAPSFQIVNYPAGAFIIVEGKKEAFNFYIVREGKVSVGRQN
                     PVVGEDPNQVLGPGDFFGVVAAMSQHPQIESARALTNVSLISVSYDQFGTLIQRNTPV
                     AMKIIRYFSMKLRQFDQTITRLSFKNTVSEDLNQLYNMGEYYFAQGNTSHAAYAYQAY
                     LQYLPNGQFAPMAKKKLMTLNLPLTMPHEDETKFNRFYKDNRVIFCEHEPGKELYIIQ
                     SGKVKISKIVNSNEVMLAVLENGDIFGEMAILDNKPRSASAVAFGDVTVLAINKANFE
                     GMVKAQPQLATRLITLLSERIWIAYKQLANLMIRDPAGRIADTLLTLAEKNRAKIAPK
                     TPYNFEIGVADVLKMVGLAENRDEKILLQLFDGNKFLMVKDDQIRTTDLGELEKLTQY
                     YVKMSAKEAKIAKDKIR"
BASE COUNT         1000 a          890 c          716 g         1182 t
ORIGIN      
        1 tatgcatccg gaagacaaat acgctaagcg ctttgacatt gatcttctca gatgcatatt
       61 ctgcggtctg tgtgaagaag cctgtccgaa gggagctatt tatctggatg gaaacggtga
      121 gatggcttca gacaacagaa acgatctgat tcttacaaaa gaaaggatga cacagaaaac
      181 cgggggacca atcctcgggc gcagaatctg attctgcgtc attaaatcac tcagagttta
      241 gtaagaacct ctactccgct ctgagtgaca agtacagtat gctcaaactg agctgaccat
      301 ttcccgtctt ttgttctgac tgtccacttg tccttcttat ccgtatatac ctgatacccg
      361 cccagattca ccatcggttc tatcgtgaac gtcattcctg gttccagttt tgcaagtttt
      421 ctcacctgcc ggaaatgagg aacctgaggg tcttcatgga aacttcttcc aatcccatgc
      481 cccatcaaat cccgaacaat tccgtatctt ttaggagtaa gaaaatcatc aattgcattt
      541 gcaatatcat ccactctgtt tccgggtctg acctgctcta tgcctatcca cattgctgct
      601 tcagtgtcct gaacaagttt ttccacttca ggcagagttt tcccaatgat gaatgtcttg
      661 gaagtatccc catggtaacc gtccactata ggagtcacgt caatattgat aatgtcccct
      721 tccttgagca catcctcttt tctcggaatt ccgtgacata ctacatcatt tacagaagtg
      781 cagatggatt taggaaatcc tttgtaaccg agaggcgctg attttgcacc ttttttcaga
      841 gtgaattcat gaatcagatc attcagttca attgtactga ctccctcttt cacatacgga
      901 gcaatgtatt ccaaaatctc cgcagcaaac ctgcaggcac tgcgcatctt gtcgatttct
      961 gttctgttct taatataaat cataattatt aaaatgtaac aaataatgcc tttgcattaa
     1021 aggcatcttc aaacatttcc ttcttctgct gtgcagacca gatatcaata tgaggatcaa
     1081 catccttcct gcatctgatc ttgaacaata ctgtcttatc ctgcacttcg caggagattt
     1141 cttcagcaat attctggtgg cttctgtcga ggccgtctgc aattctcagt attcctgcaa
     1201 gttttctgac agtattctga gcagatgctc cgagagaagc aaactccata tgctttggct
     1261 tgggtggact ttttctgtgg taccttgcta tctgagcaat aatttcgatt tcctcaaaat
     1321 tgaaacccac cataaattct gaatttcgga taatataata actgtgctta tggtgagagc
     1381 ttctggaaat tccgaatccg acttcatgaa gcatacaggc ggcctgaaga tactctcttt
     1441 cttccttggt gcatttatga aaagacttca gatcatcaaa gattttcatc gaaagctgag
     1501 tcaccttatc cacatgatcc ttttcatatg gaaatgcatt aaacagactg ttcactgcct
     1561 tcatacggat attgtcgaga ggataaggct ctgaattggt ctgaccttca aatttcctga
     1621 tggaatcata aatgatccct tcacggagcg catagtcgga gacagtcagc tgctttattg
     1681 aaaaattctt gaatatttct tcgaggataa tgcttccggc aacaatgata tcagcacgtt
     1741 tggcatcgag gccggggagc ttagttcttt ttttgagagt atcagcatca tcaagaatct
     1801 tgctcacctt ggcaaattcc tctgcagtga atgaaaaatt attcagagtt ctttgaggtt
     1861 cttctcctct gtttgcaatt accatactcg caattgtctg aatggttccg gaactgccga
     1921 tgactatttc aggagagtgt ttttcaatct ctcttctaat aggagcaatc ataccttcca
     1981 catagaacct gcactgtttt ttttccgaat ctctgtcagg atcaccgaag aagaatttgt
     2041 ccgtaagacg aatcgcacct aacttgaaac tctgtgcata atcgacctga cctttagatc
     2101 cgaccagaag ttcagtactt ccgccgccga tatcaattag aaaaaccttt ttatcgaata
     2161 cgggaagtcc ctgaagcact ccaaaataga tgagcctggc ttcttcaaat ccgctgatga
     2221 cttcaatgtg cagaccgact tctttttctg atctttccaa aaagagctga cgatttttag
     2281 cttctctaag ggcactggtg gcaattgcac ggatctgagc gttatgcata tcggcaagac
     2341 ccttgaaccg cttcaggcac ttgatacctc ggtccattgc ctcaggagtg ataaaatcat
     2401 cacccccgct tccgcttccc agtcggacag attctttctc ttttgtcaga gactcaaaag
     2461 taccgtccga tttaactctt acaacaataa tatgaaagga attcgttcct aaatctattg
     2521 cagcgagatt tctttctttc attattgctt catagtatta tctgatcttg tcttttgcta
     2581 tcttagcttc tttagcagac atcttcacat agtactgagt caatttttcg agttctccca
     2641 aatccgttgt tctgatctga tcatccttca ccatcagaaa tttatttcca tcaaagagct
     2701 gcagaaggat cttttcatcc ctgttttctg caagtcctac catcttgaga acatcggcaa
     2761 caccgatttc aaaattgtac ggtgtcttag gtgcgatctt tgctctgttt ttctctgcca
     2821 atgtaagaag tgtatctgcg attcttcctg caggatctct gatcatcagg ttggcaagct
     2881 gcttgtacgc aatccagatt ctttccgaaa gaagagtaat cagtctagtt gccagctgag
     2941 gctgagcctt gaccattccc tcaaagtttg ctttattgat tgccagaacg gtcacatccc
     3001 cgaaagcaac tgcggaagct gatctgggct tattgtccaa gatagccatt tctccgaaaa
     3061 tgtctccatt ttccagaacc gcgagcataa cctcattgct gtttacaatt ttggaaatct
     3121 ttacctttcc tgactgaata atataaagtt cttttcctgg ctcatgctcg cagaaaatca
     3181 ctcgattgtc tttataaaac ctgttgaatt ttgtttcgtc ttcatgaggc attgtcagag
     3241 gaaggttcag agtcatcagc tttttctttg ccattggagc aaactgccca ttcggaagat
     3301 actgaagata agcctggtac gcataggcag catgggacgt attgccctgc gcaaaatagt
     3361 attctcccat attgtagagc tgattcagat cttcactgac cgtgttttta aaagaaagcc
     3421 tagtgattgt ctgatcgaac tgtctcaatt tcattgagaa atatcgaata attttcatcg
     3481 caacaggagt gtttctctga atcaatgtac caaactgatc ataactgact gaaatcagtg
     3541 atacattcgt caaagctctc gcggattcaa tttgagggtg ctggctcatg gctgccacta
     3601 caccgaaaaa gtcgccaggc cctaaaacct gattcggatc ttctcctacc acagggttct
     3661 gtctgcctac gctgactttt ccttccctga cgatataaaa attgaatgct tctttcttcc
     3721 cttcaacaat aatgaaggcc cctgcgggat aattcacaat ttgaaaacta ggtgctgctc
     3781 ctgacatg
//
LOCUS       DAOQGJ010000121         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13568_length_3782_cov_3.452106, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000121 DAOQGJ010000000
VERSION     DAOQGJ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13568_length_3782_cov_3.452106"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1013)
                     /locus_tag="PK683_02885"
     CDS             complement(156..1013)
                     /locus_tag="PK683_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07421.1"
                     /translation="MKAHHTGSGSDGAPNSNARFIDPFTGKREKPANYGIMTYDLQKG
                     ERKNRNVHTVSAFGEVIFGEGQFALNATASYIYYDQKDRSDAARIGKVYLGAKYLPFF
                     SLDKNYFFVLEGRLGFPSGPDTDRFAGGNYYTGTANFTAGYKLDKFAFIGKISGIFPL
                     SKLSPNNSNNDDGIPYYLRTSVQSQEHNEIQLKKAGVYSVFINYFAHPNISLFTGFLY
                     RTPYYGVEKETSTADRIPAFYKEGSIGISYNFSEKYTLSTMYRYPIDRGREFRPYESA
                     LTTAFTLEF"
     gene            complement(1097..1936)
                     /locus_tag="PK683_02890"
     CDS             complement(1097..1936)
                     /locus_tag="PK683_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07422.1"
                     /translation="MKALVFLSAACFFSGCTFGSEGNSLLEKQIIYSRILSLYTTSDS
                     FISSNLYFYDDRADSVISEFTVGSGTPSADYTVKLNSDSKINSVGFDFYLDPPSGPFS
                     QMRHTDTTNAGSSADVSYASPFSFRSSETVSISSPSNINLSTGPAFKYIVSAFAKAKR
                     GILRRVQLEPASIVFSGTVKGTAKTIPFTVILPSPSIALNLNCRISVSPGGTVMTDLR
                     LKTSGVFKDNGSPLLSALYSAGTDGVQFYVGPTVNSSYYSIILKNLTETDVFKEELCS
                     SVN"
     gene            complement(1933..3129)
                     /locus_tag="PK683_02895"
     CDS             complement(1933..3129)
                     /locus_tag="PK683_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme enzyme"
                     /protein_id="HNH07423.1"
                     /translation="MKRIHIFFSLPVIFSFCSVKSSDSEKNKKFLIGAGILASMSGYN
                     WNLPAGFPVPSVPAENPITDAKVKLGRFLFYEKKLSGNETQSCGSCHLQSIAFADGKP
                     VGVGSTGQSHTRNAQHLSNAAYHTRITWSNNTLKTLEAQARVPMFGISPVELGLSGDA
                     YLTKLKSDSRYKELFAAAFGAGDDAYSEQNVRFSIASFIRSMISGNSAYDKYAYQKKT
                     DALTASQIRGLNIFFGETAECFHCHGGFNFTDTSTHSTASVAEFAYHDNGNRSLTEYN
                     SFPDNKKGLYEITLNTTDIGRFRAPSLRNVALTYPYFHDGSVDCSAGNKGTVGVYSDD
                     CATEALGKIVDHYMSGGKSPSNKDGTLIRPFSLSTQEKTDLINFLKSLTDDDFIKNKN
                     LSDPFK"
     gene            complement(3381..>3782)
                     /locus_tag="PK683_02900"
     CDS             complement(3381..>3782)
                     /locus_tag="PK683_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07424.1"
                     /translation="APQNINAMYWSWKGGYKFLKYEFKDKTTSKITQYHLGSGGCNGA
                     SATTPSTSCTQVNRPTISLSKSDKSIFNFATDNVVLDVISLLKGTDLASADNSCMAGN
                     SNAACAQILANTGVKASDGTSAGTQTAFSIK"
BASE COUNT         1063 a          804 c          804 g         1111 t
ORIGIN      
        1 gaaagaaaaa tacgcaaaga cttttgaaaa caagtatagc ggaggcagtg ccgattctct
       61 ggaaaaaaat attctctttt gccgatttac tgtaaaaatt tcttttttga gtttctgtat
      121 ctgacggaaa cactgcccaa tcttccggat tgcattcaaa actccagagt aaatgccgta
      181 gtcagtgccg attcgtatgg tctgaattct cttcctctgt cgatcgggta tctgtacata
      241 gtactcagtg tatatttttc cgagaaattg taagagattc ctattgatcc ttctttataa
      301 aatgccggaa ttctgtcagc agtggatgtt tccttctcca caccgtaata cggggttctg
      361 taaagaaacc ctgtgaacag agaaatattc ggatgtgcga aataatttat aaatacagaa
      421 tatacccctg ccttcttcag ctgaatttca ttatgttcct gcgactgtac tgaggttctg
      481 agataataag gaatcccgtc atcattgttc gaattgttcg gactcagttt tgagagagga
      541 aaaatcccgc tgatttttcc aatgaatgca aacttatcaa gtttgtatcc cgcagtaaaa
      601 tttgcagttc cggtataata gtttcctccg gcaaaccggt cggtgtcggg acctgacggg
      661 aaaccaagtc ttccttccag aacaaagaaa taatttttat ccaaactgaa aaacggaagg
      721 tattttgctc ctaaatatac tttgccgatt cttgctgcat cacttcggtc tttctgatcg
      781 tagtagatat aggaagcagt ggcattcagt gcaaactgcc cttctccgaa aatcacttct
      841 ccgaatgctg atacagtatg aacatttctg tttttccgtt ctcctttctg cagatcatat
      901 gtcataatcc cgtaatttgc aggcttctct cttttcccgg taaacggatc aatgaacctt
      961 gcatttgaat tgggagcacc gtcagagcct gagccggtat gatgtgcttt catgatagaa
     1021 aatgatcctg taattataaa aaacactgga atgcagaaaa caagattgtt ccgaagcaca
     1081 ctcaggcttc tcaaaatcag ttgacagaag agcatagctc ctccttgaaa acatctgttt
     1141 ctgtcagatt tttcagaata atgctgtaat aactgctgtt cactgtcgga cctacataaa
     1201 actgcactcc gtcagtaccc gcactgtaca gagccgaaag aagaggagat ccgttatcct
     1261 tgaaaactcc ggatgttttc agcctcagat cagtcattac agtgccgccg ggggaaacag
     1321 aaattcggca gttaagattc agtgcaatcg agggagatgg cagaatgact gtaaaaggaa
     1381 ttgtctttgc tgttccttta acagtgcctg aaaacacaat ggaagccggt tcaagctgaa
     1441 ctcttctgag gatgcccctt tttgccttgg caaatgcgga gacaatgtat ttaaatgcgg
     1501 gtcctgtcga aaggtttata tttgacggtg aagaaatact gactgtctct gaacttctga
     1561 aactgaacgg agacgcataa ctgacgtctg ctgaggatcc ggcatttgtg gtatcggtat
     1621 gacgcatttg agaaaaaggt cctgacggag gatccagata aaaatcaaat ccgactgaat
     1681 taatttttga atccgagttc agctttacag tgtaatcagc agaaggagtt ccgctcccga
     1741 ctgtaaactc agaaatcaca gagtctgcac gatcatcata aaagtacaaa ttcgaactga
     1801 tgaacgaatc cgaagttgta tataaagaga gtatcctgga atatatgatc tgtttttcca
     1861 aaagactgtt tccttccgag ccgaaagtac agcctgagaa aaaacaggcc gcacttaaga
     1921 atacaagggc cttcatttaa aaggatccga gagattcttg ttcttgataa aatcatcatc
     1981 agtcaggctt ttcagaaaat taatcaaatc tgtcttttcc tgtgtgctta aggaaaatgg
     2041 cctgatcagt gttccatcct tattggaagg ggatttgcct ccgctcatat aatgatctac
     2101 aatcttccct aatgcttctg ttgcacagtc atcagaataa actcctactg tccccttgtt
     2161 ccctgctgaa cagtcaacgc ttccgtcatg aaaatacgga tatgtcaggg caacattccg
     2221 aagagaagga gcccgaaatc tcccaatatc tgtagtattc aaagtgattt catacagacc
     2281 ttttttattg tccggaaaac tgttgtattc cgtaagtgac cggtttccgt tgtcatgata
     2341 ggcaaattct gccacagagg ctgtgctgtg agtcgaggta tcggtaaaat tgaaacctcc
     2401 gtgacagtga aaacactctg cagtttcacc gaaaaaaatg ttcagaccgc ggatctgtga
     2461 agcagtcagt gcatctgttt ttttctggta ggcatacttg tcatacgctg aatttcctga
     2521 aatcatagaa cggataaatg aggcaatcga aaaacgtaca ttctgctctg aataggcatc
     2581 gtcacccgca ccgaatgccg cagcaaaaag ctctttgtac ctggaatctg atttcagttt
     2641 ggtgagataa gcgtctcctg aaagtccaag ttccactggg ctgattccga acattggaac
     2701 ccttgcctga gcttccagtg tcttcaaggt attattgctc caggtgattc ttgtatgata
     2761 tgcggcattg gaaagatgct gtgcattcct tgtatgagac tgtcccgttg accctactcc
     2821 aacaggtttg ccgtccgcaa acgcaattga ctgaagatga cagcttccgc atgactgagt
     2881 ttcatttccc gaaagttttt tctcgtaaaa aagaaaccgc ccaagtttga cttttgcatc
     2941 tgttatcgga ttctctgcag gtactgaagg taccggaaat cctgcaggta gattccaatt
     3001 gtatccggac atagatgcca gtatgccggc accaataagg aattttttat tcttctcgct
     3061 gtccgaagac tttacactgc agaatgaaaa gattactggt aatgaaaaaa aaatatgaat
     3121 tcgtttcatg gcacgaatct cctttttgac tgtatgatct caggagaccc ttgctttgtt
     3181 caatgaccgc ctgagtgttt ctctgactgt gcaaaagctt actttcggga tataaacgcc
     3241 tgccggacga tgaacgcaga aagtatatta gatggagctg atatattgaa ttgacttcag
     3301 tattacggaa atgaattcaa attatccttt gcgttcttcg taggggaaat cagcctttgc
     3361 acataatcat caactgctcc ctacttaatg ctgaaagctg tctgtgtacc ggcggatgtt
     3421 ccgtctgaag cctttacgcc ggtatttgcc agaatctgag cacatgccgc attgctgttt
     3481 ccagccatac agctgttgtc tgcagatgcc aggtctgtgc ctttcagaag acttattaca
     3541 tcgagtacga cattatccgt tgcaaaattg aaaatggatt tgtctgattt tgaaagagaa
     3601 attgtaggcc tgtttacctg agtgcaggaa gtggaaggag ttgttgcact tgcaccgttg
     3661 catccgccgc tgcctaaatg gtactgagtg atcttggaag tggttttatc cttgaattca
     3721 tactttagga atttgtaacc gcccttccat gaccagtaca tcgcattgat gttctgggga
     3781 gc
//
LOCUS       DAOQGJ010000122         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13619_length_3772_cov_3.364810, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000122 DAOQGJ010000000
VERSION     DAOQGJ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13619_length_3772_cov_3.364810"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PK683_02905"
     CDS             <1..405
                     /locus_tag="PK683_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07425.1"
                     /translation="KFNLAGIIFEPATMLIVDDVHLNLDLLKKFLEPYKTLKIIVAEN
                     GQAAVESAQKFKPNLILMDMKMPVMNGYEAAKIIKNDPETAHIPVLALTASVFEHSRL
                     EISNTCDGYIQKPVSRQHLIQKISQFLKYTKD"
     gene            complement(395..1459)
                     /locus_tag="PK683_02910"
     CDS             complement(395..1459)
                     /locus_tag="PK683_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07426.1"
                     /translation="MKKKIKNALIYLLLTVSFAYSLESLFLNVNAEIQPKDEVIGNPF
                     NPSEAKPRQLSIVFGKGLFAQVSSLSSTLSNLQVSDLPSDGKLEKTIDLLERGIALKK
                     VFLVFYVFLALFSLAGFFAYTFNTWFHQATLRGVFFFTLFPVLSHMLSAGIVAQMTMA
                     GSAFFFYILNLLFLFFLIAGLIIMFRHRHFDEMTAQMLYIASNNDEESNPGKIIRETQ
                     EADRRNGFFEKLKISMHFVYIILAGFILGNLVYIPLFSLQKHYSSQFGILLFVSLIIL
                     MLFYIRNYYKAGKDEEASFSHNIAVSFSFLQYRIIRNIFLFFITSVGVIIFVISLFLL
                     LTFNTSRLKDMNVIERTINL"
     gene            complement(1462..2409)
                     /locus_tag="PK683_02915"
     CDS             complement(1462..2409)
                     /locus_tag="PK683_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07427.1"
                     /translation="MPDVRYHFIKFASRDQISKTSVLLCMALETGKKKLERIDAVVSP
                     SHETTAIDAGDTYRDCTNRIQNIILSGHSSQSSSISLINHIKNQFTQESFTFELNHYL
                     EHGDGDKLKNLMERTIQRVFGSRSEQESALFLSENTQEEMDALSPPPSTDLPKAENDA
                     REYEIPSVVPPGSKIVKFQFIVAPVTGTEVSELRLGDRILIKIQEGPDSNDILSELQL
                     KDQSGIIIPCPGIIIDLVHNEDDSTSVVVKLGEGIYGQYIEAERNIRVKMAEPSQHDR
                     EVDQYQKALQGYERGWVKPAIFLVSVITVLWIIVAFFVT"
     gene            2544..3233
                     /locus_tag="PK683_02920"
     CDS             2544..3233
                     /locus_tag="PK683_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH07428.1"
                     /translation="MSLLVEIENLKKSYFISDRGIPILSDINLKIGESEIVSVEGASG
                     VGKSTLLNIVGAMDSFDSGKVFVCGVDLGNINSEQKERFRAEKITFIFQHHLLLPDFN
                     ALENVMIPLLITGMNPVKAKAEAKDMLGRVGLKDRVSSFPSQLSGGESARVGVARALV
                     TKKKLILADEPTGNLDRENSRSLMNLILELQKEFGFSIVLVTHDLELASLASRRHKII
                     SGALQPLESVA"
BASE COUNT         1153 a          764 c          759 g         1096 t
ORIGIN      
        1 aaatttaatc tggcaggaat catttttgag cccgccacta tgctcatagt cgatgatgtg
       61 catctgaatc ttgatcttct caaaaaattt ctggaaccgt acaaaactct gaaaattatc
      121 gttgctgaga acggacaggc tgcagtcgaa tctgctcaga aatttaagcc gaacctgata
      181 ctgatggata tgaaaatgcc cgtaatgaac ggatatgagg ccgccaagat tatcaaaaat
      241 gatccggaaa ctgcacacat ccctgtactt gccctgactg cttccgtatt tgaacacagc
      301 agactggaaa tcagcaatac ctgtgacgga tatatacaga agcctgtttc aaggcagcat
      361 ctgattcaaa aaatttcgca gttcctgaaa tacactaaag attgatcgtt ctttcaatca
      421 cattcatatc cttcagtctg ctcgtattga aagtcagcag aagaaaaaga gaaattacaa
      481 atatgatcac tcccacagaa gtaataaaga aaaggaatat atttctgatg attctgtact
      541 gaagaaatga aaaactcact gcaatattgt gagaaaaact ggcctcttca tccttacctg
      601 ctttataata atttcttata taaaagagca taagtataat caaggataca aagagaagaa
      661 tcccaaactg agatgaatag tgtttctgaa gagaaaacag cggaatatag actagatttc
      721 ccagaatgaa cccggcaaga atgatatata cgaaatgcat tgaaattttc agcttctcaa
      781 agaagccgtt tcttctgtct gcttcctgag tttcccggat gatttttccc ggattgcttt
      841 cttcatcgtt gtttgaagca atataaagca tctgcgcagt catctcgtca aaatgtctgt
      901 gtcggaacat tataatcagt ccggcaatca gaaaaaaaag aaaaagcaga ttcaggatat
      961 aaaagaaaaa tgcagatcca gccatagtca tctgtgcaac aatgcctgcc gaaagcatat
     1021 gagaaagcac cgggaagaga gtaaaaaaga atacacctct gagcgttgcc tgatgaaacc
     1081 atgtgttgaa tgtgtatgca aaaaaacctg ccagagaaaa aagagcaaga aacacgtaaa
     1141 atacaaggaa tacttttttc agtgcaatgc ctctttccag aagatcgatt gttttttcca
     1201 gttttccatc ggaaggaaga tcagaaacct gaagattgct caaagtggag ctcaaagagc
     1261 tgacctgcgc aaaaagtccc ttgccgaaga caatgctcag ctgtctgggc tttgcttcag
     1321 aaggattaaa cggatttcca atgacttcgt ctttcggctg aatttcagca tttacattca
     1381 ggaaaagact ttccagactg taagcaaatg atacagtcaa taatagatag attaatgcat
     1441 tcttaatttt tttcttcata ttcaggttac aaaaaaggcc actattatcc agagaaccgt
     1501 aatgacactt actaaaaaga tggctggctt gacccatcct ctttcatagc cctgcagtgc
     1561 tttctgatac tgatccacct ctctgtcatg ctgggatggt tcagccatct tgactctgat
     1621 atttctttca gcttcaatat actgcccgta aatgccttca cccagtttta caacaacgct
     1681 tgttgaatca tcttcattgt gcacaagatc aataatgata ccgggacatg gaataatgat
     1741 tcctgactga tccttgagct gcagttccga aagaatatca ttagaatcag gaccttcctg
     1801 aatcttgatc agtattctgt cccccagcct aagttcactg acttcagtac ctgtgacagg
     1861 tgcgacaatg aactgaaatt tgacaatttt tgagccggga ggaactacac ttggaatttc
     1921 atattctctt gcatcatttt ctgccttggg cagatctgtg cttggcggag gagagagtgc
     1981 atccatttct tcctgagtat tttccgaaag aaaaagagct gattcctgtt cacttctgga
     2041 accgaaaacc ctttgtattg ttctttccat caggtttttc agtttgtctc cgtctccgtg
     2101 ttcaaggtaa tgattgagtt cgaaagtaaa actttcctga gtaaactgat tttttatatg
     2161 attgatcagg ctgatggaac tgctttgaga agaatgtccg ctcaaaataa tgttctgaat
     2221 tctgttggta cagtctctgt aagtatctcc tgcatcaatt gcagtcgttt catggctggg
     2281 agaaactaca gcatcaattc tttccagttt tttctttcct gtttcaagag ccatgcaaag
     2341 cagaacagaa gtcttggaaa tctgatctct gctcgcaaat tttatgaaat gatatcttac
     2401 atctggcatc tggacttaac cttttaattc actatgaaaa tcattccttt gatgtaaata
     2461 atttttaaga aatcctgatt ggattcaggc taaaaattag taaatcattg caagagccgc
     2521 ctagtctgag attttcagtc attatgagcc ttttagttga gattgaaaac ctgaaaaaat
     2581 cctactttat atcagaccga ggtattccta tcttatctga cataaacttg aagattggag
     2641 aatctgaaat tgtctcagtg gaaggagcat cgggagtcgg taaatcaact ctgttaaata
     2701 tcgtcggtgc gatggacagt tttgacagcg gcaaggtctt tgtctgcgga gtggatctgg
     2761 gcaacatcaa ttctgaacag aaagaacgct ttcgcgcaga gaaaattact tttatatttc
     2821 agcatcatct tcttcttccg gattttaatg cactggaaaa tgtaatgatc cctcttctca
     2881 tcacaggaat gaatcctgtc aaggcaaagg ctgaagcaaa ggatatgctt ggacgggtag
     2941 gcctgaaaga cagagtatcc agttttcctt ctcagctttc cggcggagag agtgccagag
     3001 taggagttgc aagagcctta gtgactaaaa agaaactgat tcttgcagat gagccgactg
     3061 gaaacctgga cagagagaat tccagaagtc tgatgaatct gatcctggaa ctgcagaagg
     3121 aattcggttt ttcgattgta ctggtcactc atgacctgga acttgcctct ctggccagcc
     3181 gcaggcataa aattatttcg ggcgcccttc agcctttgga atcagttgcc tgattcactg
     3241 cagaatctgc ggactgaaga aagaggactt tcttcagttc ggaaaaacgg aatgcgctga
     3301 ctgttactct tctttttttg aaaattggcg ttctgcctac agattcagtg caaattcgaa
     3361 aaaaatttcc tccctcccgt attttctgaa tgatgcaggc atagatgatt ccggctggaa
     3421 aactgcatct gcagaacagt gtctctcctt tatcctatcc ttaggtgtct cagtcagata
     3481 cagattttcg agaaacctga ggggttttca ttttccagga agaagcaatc gcgaaaggat
     3541 caaggaagat tcagtaaatt ttctgattaa aaaagctgca gaaaatcaat tccaatacag
     3601 actgaacgat gaagatcacc tgaggatctt cagattcatt cagcggactg agtcagtcga
     3661 ggatatttta tatatacctg atatttttca ggacagagat attttctgct ttctgccccg
     3721 tcttggattt gtcagttcct gtcccacaaa cctgggaaac ggaaacaagc tt
//
LOCUS       DAOQGJ010000123         3770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13630_length_3770_cov_3.231494, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000123 DAOQGJ010000000
VERSION     DAOQGJ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13630_length_3770_cov_3.231494"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PK683_02925"
     CDS             <1..273
                     /locus_tag="PK683_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNH07429.1"
                     /translation="GDREHYVSKVNFAPKKINDTDTNLQNVHFKKFCDEPYIRLYASR
                     DISVGEELYVSYGDEYEYDFMNYKEVQDFFLKKTGIKKKDTFTYKP"
     gene            285..752
                     /locus_tag="PK683_02930"
     CDS             285..752
                     /locus_tag="PK683_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07430.1"
                     /translation="MIQLQHKIIHLGMISGVSILSALFFYLKQQQGKDGIHPNETLQY
                     IAMGLGFVFLVLGQILTRKMGAEFKPKEPQSAKEYVSRYIVLKLIQWATSEGGALLNL
                     VIFFLTGFVHNFYLGVGIVIVIALTAPSDRDFYSMFGISENQFTAKPKKADFE"
     gene            749..1387
                     /locus_tag="PK683_02935"
     CDS             749..1387
                     /locus_tag="PK683_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNH07431.1"
                     /translation="MKVEIWSDIICPFCYIGKRRFEAALERFSGKDSVEVIWKSYMLD
                     PDLLPVPGQDIYDYLAKAKRQSREWSIAMHRNVTEMAAEAGLKYDFDRTVVANSRDAH
                     RLIQLAKEQGRGNAVQELLFSAYFTEGKDVSNFETLAELGQAAGMNPDDVRSLMHSYR
                     FSGQVYEEAEEGVKLGLRGVPFFVIDRKYGASGAQAPETFLKILNEASGISG"
     gene            1585..1971
                     /locus_tag="PK683_02940"
     CDS             1585..1971
                     /locus_tag="PK683_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07432.1"
                     /translation="MTKVPFGLNKKEMEILQKCSIEYAPKSLDTVLKKSSEYFDSVRK
                     AASENSAVNLRLANAILERIQKIPKFRNQLSTEGLFWIKGGAAYFFMAENAHILSSFE
                     DHTEVWNACAKIAGLDELSIWMEDFN"
     gene            2123..2887
                     /locus_tag="PK683_02945"
     CDS             2123..2887
                     /locus_tag="PK683_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07433.1"
                     /translation="MNENSAVQNSIEKFCDSYREALNAEAEKEKYRDFDPLWLGASHN
                     SWDLIPEKIRTAMQYGEIDLFFGDRELKISLYPKKDFPGLSDVLRFFRFRAEIYYSGH
                     YHYIFSKKYSTEQNLERLCGYWKPFLDSFSPAEIDGEICLDIADILQNSSLSWNCHWK
                     FRDTVLDRYAGDQIDYNSFFVSHFFSKENSESFETFVRSMQKRGMNRSETWKLFSPSG
                     ERIFSLAAVLEFDEYFSRFILETGTEFLDFYIYGRR"
     gene            3085..3504
                     /locus_tag="PK683_02950"
     CDS             3085..3504
                     /locus_tag="PK683_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07434.1"
                     /translation="MIDMNTLETYDALKKVNFTEEQSRELVRFFGKTLEADPASKRDL
                     SETELRLLKEIRELDVKLTEIPASSKRDLSETELRLQKEIEGIRLEIKELDIRGREAD
                     SRIIQTISDSKVEILKWVAGMLIANTGLIVALSKLLK"
     gene            complement(3564..>3770)
                     /locus_tag="PK683_02955"
     CDS             complement(3564..>3770)
                     /locus_tag="PK683_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07435.1"
                     /translation="EEGREEGELNSFYLSIEMALEAKFSSKGLALLPEIRTIKDISVL
                     QNILKAVLKAKDLKEISSVIKKAR"
BASE COUNT         1129 a          679 c          874 g         1088 t
ORIGIN      
        1 ggagacaggg aacactatgt atcgaaggtg aattttgctc caaagaaaat caatgataca
       61 gatacaaatc tgcagaacgt gcattttaaa aaattctgcg atgaacctta tatacgtctg
      121 tatgcttcca gagatatcag tgtcggcgaa gaactttacg taagctacgg agatgaatat
      181 gaatatgatt ttatgaatta taaggaagtg caggattttt tccttaaaaa gacaggcatc
      241 aaaaagaaag atacttttac atataagccg taaggagaaa aagaatgatt cagcttcagc
      301 ataagattat ccaccttgga atgatttcgg gagtcagcat tctttctgca ctgttttttt
      361 atctgaagca gcagcagggg aaagacggaa ttcatccgaa tgaaactctt cagtacattg
      421 caatggggct gggttttgta ttccttgtcc taggacagat ccttacaaga aagatgggag
      481 cagaattcaa gcccaaggaa cctcaaagtg ccaaagagta tgtcagcagg tatattgtcc
      541 ttaagctgat tcagtgggcg acatcagaag ggggtgcact tctgaatctg gtgatttttt
      601 tcctgaccgg ttttgttcat aatttttatc tgggtgtcgg cattgttatt gtaattgcac
      661 tgactgctcc ttctgaccgg gatttttatt cgatgttcgg aatcagtgaa aatcagttta
      721 ccgcaaaacc gaaaaaggca gattttgaat gaaagtagaa atatggtctg atattatatg
      781 tcctttctgc tatatcggaa aaagacgttt tgaagctgca ttggaaagat tttcaggaaa
      841 ggattcagtc gaagtgatct ggaaaagtta tatgcttgat cccgatcttc ttcctgttcc
      901 cggtcaggat atctatgatt atcttgcgaa agcgaaaaga cagtcgaggg aatggtcgat
      961 agcaatgcac aggaatgtga ctgagatggc ggccgaagca ggactgaaat atgactttga
     1021 caggactgta gtcgcaaatt cgagagatgc tcacagactg attcagcttg caaaggagca
     1081 gggcagggga aatgcagttc aggaacttct tttttctgcc tatttcactg aaggaaaaga
     1141 cgtcagtaat ttcgaaacgt tggccgaact aggacaggct gcaggaatga atccggacga
     1201 tgtccgctct cttatgcatt cctacaggtt ttccggtcag gtttatgaag aggcagagga
     1261 aggagtaaaa ctcggacttc ggggagtgcc tttttttgtc atcgacagaa aatacggtgc
     1321 atccggtgca caggctcctg aaacatttct gaaaatactg aatgaggcat caggaatatc
     1381 aggttaaata ctgaagtcgg cagacgaaaa actgcctgta ttcaatttcg gtgattctgt
     1441 gatgctgctg cacattactc gatttttttt cagacaagac tgagtctgaa aatcagaatc
     1501 ttgaaagggc cggttttgca gttaaatata ttattcttga ttttgaatat ctgtctgagg
     1561 atatttgaga gtaaagagtg tgaaatgaca aaggttcctt ttggtttaaa taaaaaagaa
     1621 atggaaatac tgcagaaatg ttcaattgaa tatgcgccga agtctttgga tacggtgctg
     1681 aagaaatcct ccgaatactt tgacagtgta agaaaggcag cttcggaaaa ctctgcagtc
     1741 aatctgagac tggcaaatgc cattcttgag agaattcaga aaattcctaa attccgaaat
     1801 cagctttcta cggaaggact gttctggatc aagggcggag cagcatattt ttttatggca
     1861 gaaaatgcac atatactttc cagtttcgag gatcatactg aagtgtggaa tgcctgcgcg
     1921 aaaattgcag gactggatga gctttccatc tggatggaag actttaactg aatttatgtg
     1981 tatgcaacac tgatcaaagt ccatatacat attatatgct atgaattaaa ctgagttttg
     2041 caaagtcaga caggaattat tttctgttta ctttgcctgc actcattttc catcgtatta
     2101 acggaagctt gaacgaatgg aaatgaatga gaacagtgca gtgcagaatt ctattgagaa
     2161 attctgtgat tcttaccggg aagccttgaa tgccgaagcg gaaaaagaga aatacaggga
     2221 ctttgatccg ctctggctgg gcgcctcgca taacagctgg gatctgattc ctgaaaaaat
     2281 caggactgca atgcagtacg gagagattga tctttttttc ggtgacaggg aactgaaaat
     2341 atccctttat cccaaaaagg attttcctgg tctttcggat gttctccgat ttttccggtt
     2401 tcgggcagag atttattatt ccggtcatta tcattatatt ttcagtaaaa aatattctac
     2461 ggaacagaat ctggaacggc tctgcggata ctggaagccg tttcttgatt cattcagccc
     2521 ggctgagatc gacggtgaaa tctgcctgga cattgcggac atacttcaga attcaagtct
     2581 ctcctggaac tgtcactgga aattcaggga cactgtcctg gacagatatg caggagatca
     2641 gattgattat aacagtttct ttgtgagtca ttttttttcg aaagaaaact ctgaaagttt
     2701 cgaaactttc gtgagaagta tgcagaaaag aggaatgaac agatctgaaa cctggaaact
     2761 gttttccccc tcaggagaaa gaatcttttc acttgcagct gttctcgaat tcgatgaata
     2821 cttcagcagg tttatcctgg aaactgggac agagtttctg gacttctata tttacggaag
     2881 gagatgaaga agaattggat tgctgtcggc tgtatgcgtc attttgcacc gtcattcatt
     2941 ctatctccct tcgagaaatg atatgtccat agggttgggc tgcatataca tccgaaattg
     3001 cacggtcgga attttattca cccaaaggtg taattcagtg aagaaaaaaa tgagaagttg
     3061 acattttttt tacaggtgta ttatatgata gatatgaata ctctcgaaac ttatgatgct
     3121 ctgaaaaagg taaatttcac agaagaacag tccagagaac ttgtacgttt ttttggaaaa
     3181 acgttagaag cagatcctgc cagtaaacgg gacctgagtg agactgaact ccggctactg
     3241 aaggagattc gagaactgga cgtaaaactc actgaaattc ctgcctcttc caagcgggat
     3301 ctgagcgaga ccgaactccg actgcagaaa gagattgaag gaatccgtct ggagatcaaa
     3361 gagctggata ttcggggcag agaagcggac agtcggatta ttcagacaat ttctgattcc
     3421 aaagtggaga ttctcaaatg ggttgcaggt atgctcattg caaatactgg gttgattgtt
     3481 gcactgagta aattgctgaa ataggggatg gagtctttac tcggcttcta cccactgctc
     3541 aaacgagcag tcgaacataa tttttaacgg gctttcttga tgactgaact gatctctttc
     3601 agatctttcg ctttcagcac agctttcagt atattctgca gaacagagat atcttttatc
     3661 gttctaattt ctggaagtaa agccaggcct ttggaactga acttagcttc caaagccatc
     3721 tcgatgctga gatagaaaga atttaactcg ccttcttctc tgccttcttc
//
LOCUS       DAOQGJ010000124         3753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13721_length_3753_cov_3.511898, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000124 DAOQGJ010000000
VERSION     DAOQGJ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13721_length_3753_cov_3.511898"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PK683_02960"
     CDS             complement(<1..509)
                     /locus_tag="PK683_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07436.1"
                     /translation="MKYIFSLVFIVIFCAANYQIYSNTKKKTEIYRKSPPFLAFDHTR
                     SNSSGASSSLKNIIDRNPFTAWEKQNSRSGDWDLDVELDLTHRWNGEFFQSNRLNEIV
                     IVPCAYDSAPKTLKVFAYIREAINVDKDLRLPVEQELYSDEIELEDRPYVIDLEDQLK
                     LNDSRVFPEG"
     gene            complement(546..959)
                     /locus_tag="PK683_02965"
     CDS             complement(546..959)
                     /locus_tag="PK683_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07437.1"
                     /translation="MDPKKRAELIREGNAAFNEKNYTKAEKIFMETDYKDGLIRLGDY
                     YMYDLKMPLLAYTYYKKANHQQKIDEIFQRMLMALSEWIGKDKFKISSPAAAPKPDLN
                     PDDFTVHPILKAKAMEILKKNETAQKEGSAGKRKI"
     gene            1164..1484
                     /locus_tag="PK683_02970"
     CDS             1164..1484
                     /locus_tag="PK683_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNH07438.1"
                     /translation="MPTALPDVEEETKIEEKKPGGPWRVVLWDDDEHTYDYVIEMLME
                     VCNMGLEEAYGHAVEVDTEKKTVIFSGELEHAEFIQEKVLGYGPDPRMAESKGSMTAT
                     LEQN"
     gene            1484..2179
                     /locus_tag="PK683_02975"
     CDS             1484..2179
                     /locus_tag="PK683_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07439.1"
                     /translation="MYLKLVRIFGLLTLFFFVLMFIQAGMPKPKPNARFQSVILDFEL
                     VRSKSEAYSLLGDYREKEGQETRQILDKTNLYDFPFIISYSAYTMAILLFLRSRISGG
                     QNIFLQNKIILYLGAVLSLLMCTGDILENIQLLKITEAVTAEAISDSSIKNLIIWTNI
                     KWISIFLSCILISVFYYFEKSKFRIPLTVLYLACGILGLITFIEPSRLAEPVSGLISL
                     CWTGTLVQSFSAR"
     gene            2272..2754
                     /locus_tag="PK683_02980"
     CDS             2272..2754
                     /locus_tag="PK683_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07440.1"
                     /translation="MRLKLKWSVLTAAGIFLSLTEISAKDVSSKKGESLYPDFTVEVN
                     LTPDAEKKLKQAKRTVTVNFEFGTDLGPDSTNTVQASREVKGHKGGKFSTKDLKLTGK
                     QLKKLGNNYEVNISAHSTHKGKNSLNLLNCESEGQPHVQSGIKDLLNKNIVLSCSLLK
                     "
     gene            complement(2795..3109)
                     /locus_tag="PK683_02985"
     CDS             complement(2795..3109)
                     /locus_tag="PK683_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07441.1"
                     /translation="MDILILALAVFFTSYITFFSGFGLGTVLLPVFALFFPLEAAVGL
                     TGAVHFLNNTFKFFIIKKEYDFAGAYIGNRRLEKVSLKSIQNTVTVLLFLFSLLPCAG
                     II"
     gene            3164..>3753
                     /locus_tag="PK683_02990"
     CDS             3164..>3753
                     /locus_tag="PK683_02990"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNH07442.1"
                     /translation="MKSKAVSVKDISELYEILIRIEKEDFYPTFAAVFASDEFSGQEL
                     SDFFRSRNISVFGSSSREEISENSLNSGSASVLLMDIPKEFFRVKLFPLEDSAELGRK
                     VSKWAFSEFNDPQVLLTVGGAGLTVFGDSLVQGLFESHPKYPVFGALASAKKDIYKPF
                     IFNDTESVKDGVLAWVLDGKKYRLHGTAISGWQEIGT"
BASE COUNT         1135 a          750 c          792 g         1076 t
ORIGIN      
        1 ccttccggaa acactctgga atcattcagc ttcagctggt cttccagatc aatcacataa
       61 ggcctgtctt caagttcaat ttcatccgaa tacagttcct gctccacggg aagacgcaga
      121 tccttgtcta cattgattgc ttcccggata tatgcgaata ctttcagtgt tttgggtgca
      181 gaatcataag cacaaggaac tatcacaatt tcattcagcc ggtttgactg aaaaaattct
      241 ccattccacc tgtgagtcag atccagttcc acatccagat cccagtcacc cgatctggaa
      301 ttctgttttt cccaggcagt aaaaggattt ctgtcaatga tgtttttcag ggaagagctt
      361 gcacctgatg aattggagcg ggtatgatcg aaggccaaaa aaggaggtga ttttctatag
      421 atctccgtct ttttttttgt gtttgagtat atctgatagt ttgccgcaca gaatatcaca
      481 ataaaaacta atgaaaaaat atatttcatc tctgtatcag gggtgttagg atttctgaca
      541 tcagttcaga tttttctttt tcccgcactg ccttccttct gggcagtctc atttttcttg
      601 aggatttcca tcgcctttgc tttcagaatt gggtgaacag taaaatcatc aggattcaga
      661 tccggtttgg gagcggcagc cggagaactg attttgaact tgtccttccc aatccactca
      721 ctcaaagcca tcagcatcct ctgaaagatt tcatcaattt tctgctggtg gtttgccttt
      781 ttgtagtagg tataggcaag gagaggcatt ttcagatcgt acatatagta gtcgccaaga
      841 cggatcaatc cgtctttata gtcggtttcc ataaagatct tttcagcctt agtataattc
      901 ttttcattga aggcagcatt gccttcccga atcagttctg cccttttttt cggatccatc
      961 gtattctgca gggaaccccc tgagcctcaa agccaatatt gccggaacct gaatggaaaa
     1021 taaagcaaaa aattaatcgg aatggaggaa tgatattctt ctcctctttt tgttctgtct
     1081 aatgacaaag aacggcagga aaaaggatgc attttgcttg acttatccgg cttggaattt
     1141 tagaaaagga tcaggaaaat gatatgccga cagcactgcc tgatgtagaa gaagaaacaa
     1201 agattgagga aaagaagcca ggtggtcctt ggagagtggt actttgggat gacgatgaac
     1261 atacatatga ctatgtcata gaaatgctga tggaagtctg caatatggga ctggaagaag
     1321 cctacggtca tgctgttgaa gtggatacag aaaaaaagac agtcatattt tccggagaac
     1381 tcgaacatgc cgagttcatt caggaaaaag ttctgggata tggacctgac cccagaatgg
     1441 ctgaatcgaa gggttccatg acggctacct tggaacagaa ctgatgtatc tgaaactggt
     1501 cagaatcttc ggccttctga cactcttttt cttcgtactg atgttcatcc aggcagggat
     1561 gcctaaaccg aagccgaacg caagatttca aagcgtgatt ttggatttcg aactagtccg
     1621 ttcaaaatct gaggcttaca gccttttagg agattataga gaaaaggaag gacaggaaac
     1681 caggcagata ctggacaaaa ccaatctcta tgattttcct tttattatct cctactccgc
     1741 atatacgatg gccatcctgc ttttcctcag atcaagaata tctggcggac agaatatttt
     1801 tcttcaaaat aaaattattt tatatctggg agctgttctt tcgctgctga tgtgtacggg
     1861 agatattctg gaaaatatcc agcttcttaa aattaccgag gcagtcactg ccgaagctat
     1921 ctccgactcg agtattaaaa atttaattat atggaccaat ataaaatgga tatccatatt
     1981 tctgtcctgc atcctgattt ccgtatttta ttattttgag aaatcaaaat tcagaattcc
     2041 ccttactgtt ctgtatcttg cctgcggaat ccttggactg atcactttca ttgaaccgag
     2101 cagacttgct gagcctgtat ctggtctgat ttccctctgc tggacaggga ctcttgtaca
     2161 aagtttctct gcaagataag acagatcatt ctctcccggc attgaaaata aatgataatc
     2221 gaatttacag tttcagaatc agggaaatac tggatggaaa ggagaataaa aatgagactt
     2281 aaattaaaat ggtcagttct gactgcggcg ggaatatttc tttcattgac agagatctca
     2341 gcaaaggatg tttcctcaaa aaaaggagag tccctttatc cggactttac agttgaggta
     2401 aatcttacac cggatgctga gaagaaactc aagcaggcga aaaggactgt gactgtgaat
     2461 tttgaatttg gaactgatct tggaccggat tccacaaata ctgttcaagc atcccgtgaa
     2521 gtgaaaggac acaagggagg aaaattttcc acaaaggatc tgaagctgac aggcaagcag
     2581 ctgaaaaaac ttggaaacaa ttacgaagtg aatatcagtg cacattccac tcataagggt
     2641 aaaaattcat tgaaccttct caactgcgaa agtgaaggtc agcctcacgt tcaatccggc
     2701 ataaaagatc tactgaataa aaacattgta ttgtcctgct ccctgctgaa ataaacctgg
     2761 aagagcagac tttcggctta tactagactg caaatcagat aattccggca catggaagga
     2821 gtgaaaaaag gaaaagcaga actgtcactg tattttgaat acttttcaag gaaacctttt
     2881 ccagtctgcg atttccaata taagctcctg caaaatcata ttctttttta atgataaaaa
     2941 acttgaatgt attgttcaaa aaatgtacag caccggtaag tcccacagcc gcttccaaag
     3001 gaaaaaacag tgcgaacaca ggaagaagaa ctgttccaag cccgaatccg gaaaagaaag
     3061 tgatgtaaga agtaaaaaaa actgcaagtg ccaggatgag aatatccata gttcctcttt
     3121 tttaggttgt caattttcta caatcatctg aactgattgt agaatgaaat cgaaagcagt
     3181 ttcagtcaaa gacatttctg aactttatga aatcctgatc cgaattgaga aagaagattt
     3241 ttatcctacc tttgcagctg tatttgcatc tgatgaattt tccggacagg aactgtccga
     3301 ctttttccga tccagaaata tttctgtttt cggttcgagc agcagggaag agatttctga
     3361 aaacagtctg aactccggct cagcctcagt gcttttaatg gacataccga aagaattttt
     3421 cagagtaaaa cttttccctc tggaagattc agctgagctg ggaagaaaag tttcgaaatg
     3481 ggcattttca gagttcaatg atcctcaggt gcttctgact gtaggcggtg caggtctcac
     3541 tgtattcgga gacagtctgg tgcaggggct ttttgaatct catcccaaat accctgtatt
     3601 cggggctctt gccagtgcaa aaaaagatat atataaacct ttcattttta atgatactga
     3661 atccgtaaaa gacggagttc ttgcctgggt tctggacgga aaaaaataca ggctgcacgg
     3721 aactgcaatc agcggatggc aggaaatagg gac
//
LOCUS       DAOQGJ010000125         3751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13733_length_3751_cov_2.899892, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000125 DAOQGJ010000000
VERSION     DAOQGJ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13733_length_3751_cov_2.899892"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PK683_02995"
     CDS             <1..943
                     /locus_tag="PK683_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNH07443.1"
                     /translation="IGTHSLLQPDVQFSDLGLVIIDEQHKFGVEQRETLRSKGKNPDL
                     LAMTATPIPRTLCLTLYGDLNLITIRNKPKGRKPIVTKWFKEDRRESVYSSMKKYLKQ
                     GRQAYIVYPIIEESEKTDMQSCLEGFESLRNVYFTEFRAGLLHGKMKTAEKEKVMADF
                     KKGDVDLLVTTTVVEVGVDVPNAAVLVIENADRFGISQLHQLRGRVGRGEHESFCILL
                     TKNFVSDDAEARLEAVCSSEDGFILSEKDLQIRGPGELLGTRQSGLPDFKIADLKEDG
                     SILEMAKEDSLVSSGITEDVKHEIRERFEEGSLLFPN"
     gene            1087..1590
                     /locus_tag="PK683_03000"
     CDS             1087..1590
                     /locus_tag="PK683_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07444.1"
                     /translation="MNRMFLFLMVFICSVSVYSQSSGGGSKNFGLGLSVISPTGITGK
                     YWLDDKKAIEGTLGLGYQGDRWGNYLHGVLLFNLADLSPNVRFYAGGGLALAHRYYKD
                     KEIFGDLFRKNEHSQWIPGLRVPAGIAYRTDDKSLEFFGELSANLFFYRGTDFDLWLS
                     LGGRYYF"
     gene            complement(1715..2638)
                     /locus_tag="PK683_03005"
     CDS             complement(1715..2638)
                     /locus_tag="PK683_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNH07445.1"
                     /translation="MKLIRFTLMLLFCNSLVTVCSQKKISFRLSSGFKEGTYFAVGES
                     LKSIPEFEVQNSASKGSVDNIMQIMDGKADLAITQLDVLQNYAIGEPKAKDKIKILTP
                     LYGEEVHVLVNRSIKSFSDLKGKKISVGDSISGSKQTALLFLGHFDLDTTQIKIEELA
                     PDKSLELLLKKELDGMVLTAGYPVKLFSGLPDKTKEDFHFLEFYGEKYEAVKGTNLMY
                     NKGYIPAKTYSFQDEDVRTIVVTSILAVRADMSEKDVLFILKSIFSNSVKLTMQHEKW
                     NSLNKKFLQALIQRNPTLFHSAVEKNLNSLQ"
     gene            complement(2682..>3751)
                     /locus_tag="PK683_03010"
     CDS             complement(2682..>3751)
                     /locus_tag="PK683_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07446.1"
                     /translation="LEKKVSSPVFHEILAGYSDSFGLLKTKVQAGQGLYRLDSYGFLF
                     SGPAGFADLSLRISSLPFSLSFQNLVLNRRSGTLYRSADSEAGGAVLKKDKDAYFKSF
                     QMMYFIYREFRNQTYTGFFPEDKKEFLPEGNFRYAGIELNSERFFRSEISTGAFTVKG
                     KRDFRETDFNAHSVRKTDAYLGYLCLDFFFTERLSARAGGLRTSKDRNSRTDRRSNGF
                     AGILSDPGIFGGHSSFLLSENISGKSPPAFRDLDSNSRPDFENKGLKMYSVSLNYNFE
                     KNAFTGILNRAAGFTGTGYEFIFQWKYSSMNTAYPYYLLVSFCEAFLFSREENILLFS
                     SEKTEKRREFQRIYFSLGTVF"
BASE COUNT         1077 a          741 c          845 g         1088 t
ORIGIN      
        1 gataggaact cacagtctgc ttcagccgga tgtgcagttt tcggatctcg ggcttgtgat
       61 catagatgaa cagcacaagt ttggagtgga acagagagag actctccgtt ccaagggaaa
      121 aaatcctgac ctgctcgcaa tgaccgccac tcccattccg aggacactct gtctgacttt
      181 gtacggagat ttgaatctga tcacaatcag gaacaagccg aagggaagaa agccaatcgt
      241 tacaaaatgg ttcaaggaag acagaagaga gtcagtatac agttccatga aaaaatatct
      301 gaaacagggg agacaggctt atattgtata tcctatcatc gaggaatcgg aaaagacgga
      361 tatgcagtcc tgtctggaag gatttgaaag tctcaggaat gtctatttta ccgagtttag
      421 agctggactt cttcatggga aaatgaagac tgcagagaaa gaaaaagtta tggccgattt
      481 caagaaagga gacgttgatc ttcttgtcac tacgactgta gtcgaagttg gtgtggatgt
      541 acccaatgcg gcagttcttg tgatcgaaaa tgcagacagg ttcgggatat cccagcttca
      601 tcagctgaga ggccgtgtcg gaagagggga gcatgaaagc ttctgcattc ttctcaccaa
      661 aaattttgtt tccgatgacg cagaggcaag actggaagcg gtctgcagtt ccgaggatgg
      721 atttattctt tccgaaaagg atcttcagat caggggacca ggcgagcttc tgggaacaag
      781 acagagcgga ctgcctgatt tcaagatagc tgatctgaaa gaagacggca gtattctgga
      841 gatggctaag gaggattcac ttgtctcttc cggaatcact gaagatgtaa agcatgaaat
      901 cagggaaaga tttgaggaag gcagtcttct ttttcccaac tgaagcggca gatttatttt
      961 cattatatca tatcatcaga tccggaggat cattcaaaaa aagttttttt cttggtctct
     1021 gatatgcaaa tttttagatg gaatctgtca gtttcagctg atcaaatcta tttcttgcga
     1081 gttgctatga acagaatgtt tctttttttg atggttttta tctgcagtgt gtccgtctat
     1141 tcacagagtt caggaggagg ttcaaagaat tttgggcttg ggctttccgt catttctccc
     1201 acaggaatta ccggcaaata ctggctggat gacaaaaagg cgatagaggg aacccttggt
     1261 ctggggtatc agggggacag atggggaaac tatctgcacg gagttcttct gttcaacctg
     1321 gcagatctga gtccgaatgt ccgtttttat gccggcggag gactggccct ggcacacaga
     1381 tactataagg acaaagagat attcggcgat ctgttcagaa aaaatgaaca ttctcagtgg
     1441 attcccggcc tcagagttcc tgcaggtatt gcctacagaa cagatgacaa atcgctcgaa
     1501 tttttcggag aactcagtgc gaatcttttc ttttacagag gaactgattt tgatctttgg
     1561 ctttctctcg gagggagata ttatttctga agaagtatct gcctgatttt atcattataa
     1621 aaaatgtcaa ggtattaagg atttcctgag aaccgtaaaa gctctcagga attttttatg
     1681 cgagtgcagc tcacagtaag aaaagcagag ccggtcactg aaggctgttc aggttttttt
     1741 caactgcact gtggaaaagg gtaggatttc tctgaataag tgcctgcaga aatttcttat
     1801 tcaggctgtt ccatttttca tgctgcatag tgagtttgac agaatttgaa aaaatgctct
     1861 tcagtatgaa cagtacgtcc ttttcactca tatcggctct gacagcaaga atggaagtga
     1921 caacgattgt tctgacatcc tcatcctgga agctgtaagt ttttgccgga atgtagcctt
     1981 tattgtacat cagatttgtt cccttgaccg cttcatattt ttctccgtaa aactcaagaa
     2041 aatgaaaatc ttcctttgtc ttatcaggga gaccgctgaa cagtttgaca gggtaacctg
     2101 ctgtcagtac cataccgtcc agttctttct tcagtagaag ttcaagagac ttgtccggag
     2161 caagttcttc aattttaatc tgagtagtat cgagatcaaa atgtcctaaa aacagcaagg
     2221 ctgtctgctt tgatccggaa atactgtctc cgacagaaat cttttttcct ttcaagtctg
     2281 aaaaggactt aatgcttctg ttcacaagaa catgcacttc ttctccgtag agaggtgtca
     2341 gaattttaat tttatccttt gctttcggtt cgcctatggc ataattctgc agaacgtcaa
     2401 gctgagtgat tgcgagatca gcctttccgt ccataatctg cataatatta tccacgctgc
     2461 ctttggatgc agaattctgt acttcgaatt caggaattga tttcagggat tccccgacgg
     2521 caaaataggt tccttccttg aagccggaag aaagacggaa agatattttc ttctgtgaac
     2581 atactgttac aagagaatta cagaaaagga gcatcagtgt aaaacggatc agtttcatag
     2641 tgtaaagaat gcagatcctt tcagactgtc aagcaaagag ttcagaaaac tgtgcctaga
     2701 gaaaagtaaa tcctctgaaa ttcccttctt ttttccgtct tttcggaaga gaacaaaaga
     2761 atgttctcct ctctgctgaa cagaaatgcc tcacagaatg aaacaagcag ataatatgga
     2821 tatgcagtat tcatactgct gtatttccac tgaaatatga attcatatcc tgttcctgta
     2881 aaacctgccg ctctgttcag tatgcctgta aaagcatttt tctcaaaatt ataattcaga
     2941 gagacgctgt acatcttcag tcccttgttt tcaaaatcag ggcgggaatt cgaatccaga
     3001 tctctgaatg ccggagggga ttttcctgag atgttttctg aaagaagaaa ggaggaatga
     3061 cctccgaata ttccgggatc tgaaagaatg cccgcaaatc cgtttgaacg tctgtctgtt
     3121 ctgctgtttc tgtctttcga tgttctaagt cctcctgctc ttgcagaaag acgttccgtg
     3181 aaaaaaaaat ccagacagag ataaccaagg tatgcgtctg tctttcttac ggagtgtgca
     3241 ttgaaatccg tttcacggaa atcacgcttt ccttttactg taaatgctcc tgtggaaatt
     3301 tcagagcgga aaaatctttc tgaattaagt tcaattcctg catatctgaa atttccttcg
     3361 ggaaggaatt cttttttatc ctcaggaaag aaaccggtat aagtctgatt tctgaattct
     3421 ctgtagatga agtacatcat ctggaaagat ttaaaataag catccttatc tttttttaaa
     3481 actgcaccgc cagcttcaga atcggctgat ctgtaaagtg ttccggatct tctgttcaga
     3541 acaagattct gaaagctgag tgaaaacggc agactgctga ttcttaagga caggtctgca
     3601 aaaccggcag ggcctgagaa aaggaaaccg taactgtcca gacggtaaag cccctgacct
     3661 gcctgcacct ttgttttcag aagaccgaat gaatctgaat atccggccag aatttcatga
     3721 aagacgggtg atgaaacttt tttctctaga t
//
LOCUS       DAOQGJ010000126         3748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13750_length_3748_cov_2.875982, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000126 DAOQGJ010000000
VERSION     DAOQGJ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13750_length_3748_cov_2.875982"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PK683_03015"
     CDS             <1..764
                     /locus_tag="PK683_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07447.1"
                     /translation="GGAIARFTQADSIIDANSVQGMNQYMYTEGNPVNFRDPSRHKKM
                     DALMGKIASAMMMTRIFNKDDNSATNYYRLVMLSKMMSSRNKKGSWYNRSDLSTAVNW
                     TSPMNIIGAAWAGHNYISGRLMGLNTQIHQDRYGFHVTDAPHMNGGDTGINMGYFSVQ
                     AGDRRNVTKHENGHKQQFHQWGADAYTGVFGLRLTGKGAGWPETNADRRAGTFAYDRN
                     NDTLFFYLRLIDFVRSKDLNQYAQDGFIADYGYVK"
     gene            1143..1631
                     /locus_tag="PK683_03020"
     CDS             1143..1631
                     /locus_tag="PK683_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07448.1"
                     /translation="MLAFFRSSLSPEPRSQFSPLAAIAIPLFLFLSSPLFPEENRSSY
                     IRTLLDRRDLSAARSELDRHLKDSPNDPSVKFLQTELLIAESEAAESRGDIKTALEKL
                     EKVIPEYPKHSNVVLRYEDLMKRKQNGGLNGIPNIILSGSPANTSLSERVAKLEEAQR
                     FC"
     gene            2561..>3748
                     /locus_tag="PK683_03025"
     CDS             2561..>3748
                     /locus_tag="PK683_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH07449.1"
                     /translation="MNILDRFNKNIRIRTKMIMIISGVLLISIIPLAFIILNRSQDIV
                     LKMTFEVCTNLAQNISNLATEELLINETYDTTKTSISRLKSSGITGLLDSFVVNVDGE
                     YVAEMASEEQIGKQVSQKEKDYYRGLTKLDMQDTSSSRWKTKILRFSFPISILYRGQQ
                     MRVGTAVFEFDRAKVYEPVYKVRNTIILVSAILFILGIAIAFYTAIIFSKPILKLSES
                     VKRIGDGDLNHRIKLTGKDEIGQLAQTFNQMTSQIQDFTQNLENKVAERTDQLNKSLQ
                     EVQTLKIAQDGDYYLTSLLLSPLQPNNNRSSRIKTEFLIEQKKKFTFRKWDSEIGGDI
                     CITDTIKLLNKEYTVFINGDAMGKSIQGAGGALVLGVVFNAGLMRSRVEKYQGIHPEA
                     WLKE"
BASE COUNT         1174 a          738 c          877 g          959 t
ORIGIN      
        1 gagggggggc gatagcgaga ttcacacagg cggattcgat tatagatgca aattcagtac
       61 agggaatgaa tcagtatatg tatactgagg gaaatccggt gaacttcaga gatccgagca
      121 gacataagaa aatggatgct ttgatgggaa aaatcgcttc tgcaatgatg atgaccagaa
      181 tattcaacaa ggacgacaat tcagcaacaa attattacag actggttatg ctgagcaaga
      241 tgatgtccag tcggaacaag aaaggaagct ggtacaatag aagtgatctc agcacagcag
      301 taaactggac atctccgatg aatattattg gagcagcgtg ggcaggtcac aattacattt
      361 caggaagact gatgggactg aacacacaga ttcatcagga cagatatggc tttcatgtta
      421 cagatgcgcc gcatatgaat ggaggagata cagggattaa tatgggatat ttttcagttc
      481 aggcaggtga cagacggaat gtgacaaaac atgagaatgg acataagcag cagtttcatc
      541 aatggggagc ggatgcctat accggtgttt ttggtttgag acttacagga aaaggagcag
      601 gatggccgga gacgaatgcg gacagaagag ctgggacatt tgcttatgac cgtaataacg
      661 atactttgtt tttttatctg cggctgattg actttgtgcg cagcaaggac ctgaatcagt
      721 atgcacagga tggttttatt gctgactatg gttatgtaaa atgattctaa aaatggaact
      781 cttactggca ggtactcttt tccttggatg ttcagatatt cataaaggag ctgattatac
      841 gaaaagaaaa aaggaatgtg tacaaaaaaa tatgacggtt ttatctatct actctcagtc
      901 actggaaccc tctgatagaa attccagcct gcttttactt ggtgcggatg ttgaagttcg
      961 atgcagaaga aatgctcggg gagtattcgt cagcattcca aatacaggaa gtgaatcaaa
     1021 tcatggaaca ggagattcgg atggatttca ataaatggta ttatataatg attaaatgta
     1081 taataaaaat gaaaagtatt ttgacagaat tgctatacgc tgttcgccgc tttcgctgtc
     1141 cgatgcttgc tttttttcgc agttccctaa gccctgagcc ccgatcccag ttctctccgc
     1201 tggccgctat cgccattccc ttattcctct ttctgtcctc cccgcttttt cccgaagaaa
     1261 acagaagttc ctatatccgc acacttctgg acagacggga tctttctgct gcccgttcgg
     1321 aactggacag gcatctgaaa gacagtccga atgatccgtc tgtaaagttt cttcagaccg
     1381 aacttctgat tgcggaatcc gaagctgctg aatcgagagg ggatatcaag acggctcttg
     1441 agaagctgga aaaggtaatt ccggaatatc cgaaacattc gaatgtagtt ttaagatatg
     1501 aagatttaat gaaaagaaaa cagaatggcg gactgaatgg aattccaaat atcatcttat
     1561 ccggttcccc ggcgaatact tctctttcgg aaagagtagc aaaacttgaa gaagctcaaa
     1621 gattctgctg agtccggagt tgatgaaata aaaacagtgc agtgcaggga cgggaaaggg
     1681 acaatgagaa atacgggttc agtctacaat aactctgcag tatttctgcc ggaagaagtg
     1741 cctgtgagaa cagccaagcg cgaaaaaagc ggatgctgtg tgattggagt tcaggatttg
     1801 gagtctggcg ttcgggaggg ttctgtaatg ataaagtata ttagaaagat gaagactgcc
     1861 tttgcagaca gtaaggggat cggggttgaa aaatgcaata cgaagggata caagtattac
     1921 agaaaaatat atattatacc cagatttcta atctcgcaag cgtggaagcc gctgctgtgc
     1981 tccgcccttc ggactattag aaatctgggt tatacagctg ttccccgcta tcgctgccgg
     2041 ccattccctg tcagagtgta tgatagagtg cctgcgttcg ggagaattct ttgtaatgat
     2101 aaaatttgct gagaaaatgg aatgtatttt tcggaatctc tgtcctctat acgctttccc
     2161 ctgagatcca ggcgccgaac cctgatccct ccctgataac ccggcggaaa agtcaaaaaa
     2221 ttatcaaaga ccgattctat aacaaataca atgggcattt agatgccgac ccagtctgtg
     2281 aatcggctgt aaaatgacag ttgattcaaa ataatggcag ccgcaggcga agggaaaggc
     2341 agctgctgtc aggcagactg aatgaaaaac caaagagaaa gaattataca gctcaaggca
     2401 gattcactgc tcatctgcaa gattcagtac aaagcggttt cccgaaatat tcagtattct
     2461 caagatccgt attttaaaaa tgatttagac taattttgaa aaaaaaactt tacataagtg
     2521 caaggagaaa gaatatcaat ccttgctaca taaaaggcct atgaatattt tagacagatt
     2581 taataaaaat atccgcatca gaacgaagat gattatgatt atctcaggtg ttcttctgat
     2641 cagcatcatt cctcttgcat ttatcattct gaacagaagt caggatattg ttctgaaaat
     2701 gacctttgag gtgtgtacaa atcttgcgca aaatatttca aacctggcaa cagaagaact
     2761 tctcatcaac gagacgtacg atacaaccaa aacatccatc tcgagactga aaagcagcgg
     2821 gattacaggt cttctggatt ctttcgtcgt gaatgtggac ggagagtatg ttgcggaaat
     2881 ggcctcggaa gaacagatcg gaaagcaggt atcccagaaa gaaaaggact attatcgcgg
     2941 tctgacgaaa ctggatatgc aggacacttc ttcttcccgc tggaaaacta aaattctccg
     3001 attttccttc ccgatttcca tcctgtaccg gggccagcag atgcgggtag ggactgctgt
     3061 ttttgaattt gacagggcaa aggtctatga gccggtctat aaggttcgaa acacaattat
     3121 tctggtatcc gcgatccttt ttattttggg aattgcaatt gcattctata cagccattat
     3181 attctccaag cccattctga aactctccga aagtgtcaag aggatcggcg acggagattt
     3241 gaaccacaga attaaactga ccggaaagga tgaaatcggt cagctcgctc agaccttcaa
     3301 ccagatgacc tcccagattc aggatttcac tcagaatctg gaaaacaagg tggcagaaag
     3361 aacagatcag ctgaataaat cccttcagga agtgcagact ctcaaaattg cacaggacgg
     3421 ggattattac ctcacatctc ttctgctttc tcctcttcag ccgaacaata acagatcctc
     3481 acggatcaag acagagttcc tgatcgaaca gaaaaagaaa ttcacattca gaaaatggga
     3541 ttcagaaatc ggaggggata tctgcattac tgataccatc aaactcctga acaaagaata
     3601 cactgtattc atcaacgggg atgcaatggg aaaatccatt cagggagccg gcggtgcact
     3661 tgtactcgga gtggtattca atgcgggtct gatgcgctcc agagtcgaaa aataccaggg
     3721 aattcatccg gaagcctggc tgaaagaa
//
LOCUS       DAOQGJ010000127         3745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13770_length_3745_cov_2.511382, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000127 DAOQGJ010000000
VERSION     DAOQGJ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13770_length_3745_cov_2.511382"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PK683_03030"
     CDS             <1..425
                     /locus_tag="PK683_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07450.1"
                     /translation="YGPNSALIVLGEFLSHLFFLSIFPEILSYLADYQARKNQKTGDL
                     NILISYSRYSISIFGLAAPFSILMSLVKLQGSTGYFVILFVLCFCFILNVIRGGCEIY
                     KVDKSQMFRLMFSSFFFALIVPPVLCLYYLFSIVFVIF"
     gene            complement(435..1235)
                     /locus_tag="PK683_03035"
                     /pseudo
     CDS             complement(435..1235)
                     /locus_tag="PK683_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            1319..3256
                     /locus_tag="PK683_03040"
     CDS             1319..3256
                     /locus_tag="PK683_03040"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNH07451.1"
                     /translation="MKKLSFIFLSVFILTFPVSVTLSEGFSILSSFFLLIHKIRNREK
                     PNLPKEFYALLILYAVLFFTGVFQNSEIPLYKKLFRSEISYVWMLFPFWTAFELGNEF
                     KKEIIRVSAVSFLILLLSGAVSVFTPFRLAVWFQNGFSVPSSARLQHFAGNILGLNTY
                     LPVGLMNTHLTYGGLLGIFFSGVILYFLWSIFLFFRTKVSSGALLRVFSVLILTLIFL
                     SVLFYNQSRSVWIGCVFSFLIFPFSSDFLRAQIRNLFQKRKFSVRKLFLFFFLFGILF
                     SGGIYLLIQKNWMLQRALFEAGKKNTTENQRYFIYRPTMDLISKNFWTGTGSGAFRKF
                     HEQASEKYILEYEQLWYEMYITPRGHAHHDILHIQSVGGIFASLFYILFWLLVFRRLL
                     HFRNSISASFFSGFTVLFPAGFFQCYMQDAEVLLPFFVSAGMYCALTEKNIRDRIHRK
                     ELLYPLLTVISLIFLSVFLTFSRISDSPFEVYSRKIRETDPLTAEKVLSSLSGSRKEK
                     ISLDSAEKGFSIEGCLTHRYAVKNPAPRQEDYSIALEIEESSENPPVQVQVHAVERDS
                     FDQDQLYRAHSKKDLKNYEFALQKGRNLLRFEGLLSDTPSKQFPENIYFRDFHFRFVP
                     LKKKEMDLPRFDFGRLCDVKE"
BASE COUNT          953 a          785 c          753 g         1254 t
ORIGIN      
        1 actatggtcc gaattctgca ctgattgtgc ttggggaatt tctttcccat ctttttttcc
       61 tttcaatttt tccggagata ttaagttatc tcgcagacta tcaggccagg aaaaatcaga
      121 aaaccggcga tctgaacata ctgatctcct attccaggta ttccatctcc atattcggtc
      181 tggcagcacc tttttccatt ctgatgtcac ttgtcaagct tcagggtagc accggatatt
      241 ttgtaattct ttttgtactc tgcttctgct tcatactcaa cgtgatcagg ggagggtgtg
      301 agatatacaa ggtggataag agccagatgt tccggcttat gttcagttca tttttctttg
      361 cactgattgt tccgcctgtc ctctgtttat attatctgtt ttctatagta tttgtaattt
      421 tctgactctc cggtttattc tgcagatttg ggacgggaat agtctttccg aagattggaa
      481 ggcctttcca ctgttccttt ctgcatctca tacagttttg caaatttcaa aaagctcgaa
      541 tacgcgcttg agaccgcaat cacaaaaccg ggatatccgt cgagaaaccc cagcttgaat
      601 atgtaacatt ccaggaattt cacgaaaggt ttatagacag atttgataat agggaatttt
      661 atttttttgc cgaatctggt gaatcctaca attgaggaga atttgttgat tgtgtccacc
      721 tgatgagaaa gatccttgaa actgtagtgg atgatgtctc ctgaaagaga ttttcctgct
      781 cccttgattt caatgaagtc atgaggattt tcccccgtcc agactgcact tccctttctg
      841 aaaagtctgt atcttttcag cggataccat cccccgtgac ggatgaatct tcccatatga
      901 aaagtgagtc tcgggatttt ccagcctgag atttcttccg ggtcatcccg cagaatatcc
      961 tgtatttctt ttttcagagt ttcagacact ctttcatctg catcaagcga gagtatccat
     1021 tttcctgagc agagttctat cgcattgttc ttctgttcaa tatgccctct gaattccttc
     1081 tgaaatattt tcaccttggg aaattcgcgg gcaatttcca aggtactgtc tgtgctgaat
     1141 gagtccagta tgatcacttc atctgcaatt tcataaaaag aagaaatgca gtcccgaata
     1201 ttgttttctt cattgaacgt aatcactgcc gccgaaaaaa ggatttttgt attttcactc
     1261 tgacccatat taaatagaat ggaaaaagac tttcatctgc actgtcaacg atttatccat
     1321 gaaaaaactc tctttcattt ttctttcggt ttttattctt acttttccgg tatccgtcac
     1381 tctctctgaa ggattttcca ttctttcttc cttttttctg ctgattcaca aaatacggaa
     1441 ccgggaaaag ccgaatcttc cgaaagaatt ttatgcgctt ctgatattgt atgctgtttt
     1501 attttttaca ggagtctttc agaattcaga aatccccctt tataaaaaac ttttcagatc
     1561 ggagatttcc tacgtatgga tgctttttcc tttctggaca gcgtttgaac tgggaaatga
     1621 atttaagaag gagatcatcc gtgtttccgc cgtttccttc ctgattctcc ttctttcggg
     1681 tgcggtttct gtgtttactc cattccgcct ggctgtatgg tttcagaacg ggttttctgt
     1741 tccctcatct gcaagactgc agcattttgc aggaaatatt cttggactga atacctatct
     1801 tcctgtggga ctgatgaaca ctcatttgac atacggagga cttctcggca ttttcttctc
     1861 cggcgtgatt ctgtatttcc tctggagcat ttttcttttt ttcagaacca aagtcagttc
     1921 aggagccttg ctgagagtgt tctctgttct gattcttacc ttgatctttc tctcggttct
     1981 cttctataat cagagcagat ccgtctggat cggatgtgtt ttttcatttc tcatttttcc
     2041 attctcttcc gatttcctca gagcacagat cagaaatctt tttcagaaaa ggaaattttc
     2101 agtccggaaa ctgtttctgt ttttctttct gttcggcatt cttttttcag gagggattta
     2161 tcttctgatc cagaaaaact ggatgcttca gagagcgctg tttgaggcag gaaaaaagaa
     2221 caccacggag aatcagagat acttcatata cagaccaaca atggatctga tttcaaaaaa
     2281 tttctggaca ggcactggat caggagcatt cagaaaattc catgagcagg cttctgaaaa
     2341 atacatactg gaatatgagc agctctggta cgaaatgtac atcactccga ggggacatgc
     2401 tcatcatgat attctgcata tccagtccgt cggaggaata tttgcatcat tattctatat
     2461 tttattctgg ctgcttgtat tcagaaggct tctgcatttc aggaacagta tatctgcctc
     2521 ttttttttct ggattcacag ttctttttcc ggcaggtttt tttcagtgtt atatgcagga
     2581 tgcagaagtt cttcttcctt tttttgtttc agcagggatg tactgtgcac tgacggaaaa
     2641 aaacatcagg gacagaattc atagaaagga gttactgtat ccattactga ctgtaatttc
     2701 actgatattt ctttctgttt ttcttacatt cagcaggatt tcagactctc cctttgaagt
     2761 gtattcaagg aagatcagag agactgatcc tctgactgca gaaaaggtgc tttcttctct
     2821 ttccggaagc agaaaggaaa agatcagcct ggattctgca gagaagggat tcagcattga
     2881 aggatgtctg actcacagat atgcagtgaa aaatcccgca ccgagacagg aagactattc
     2941 tattgctttg gaaatagagg agagttcaga aaaccctccg gtgcaggtgc aggttcatgc
     3001 agtggaaaga gattcgtttg atcaggatca gctgtacagg gcacattcca aaaaagatct
     3061 gaaaaattat gaatttgcac ttcagaaagg aagaaatctg cttcggttcg aagggcttct
     3121 ttcagatact ccttcgaaac agtttccgga gaacatctat ttcagggatt ttcatttccg
     3181 gtttgttccg ttgaagaaaa aagagatgga tcttccccgg tttgattttg gaagactttg
     3241 cgatgtgaag gaatagtcca gcctgcacta aagattcttt aatttatctt acacctacgg
     3301 ctgcagctgg agcacgttgt tgacagcatt tgcctgccgg cttctttcgg gtccgcatat
     3361 atggaggctg ctccaaactt aaaatcgact gccgccctcg caatgtctga aaccggtacc
     3421 ggcattcaga agcttcctga actcccgcaa gcctggaaga agctttcttt actgattcat
     3481 ggcaagctgt taaactgcca gattcctctg atacttcgta aaaaatatat gttctatata
     3541 ttatattcca ttttgtaagg tatgttctaa gatgtttttc cattcgggac tttggatagg
     3601 catatttcca ggagaactca ctgcggtcac cgagcgatgc accggaccaa aagccttgta
     3661 ggaaacggcg gggctttttt gtttttaaaa gaataaactg tgaaattggg aaaaaatata
     3721 tctatataaa ctgcagcgtc tcctt
//
LOCUS       DAOQGJ010000128         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13855_length_3731_cov_2.822361, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000128 DAOQGJ010000000
VERSION     DAOQGJ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13855_length_3731_cov_2.822361"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /gene="tmk"
                     /locus_tag="PK683_03045"
     CDS             complement(<1..330)
                     /gene="tmk"
                     /locus_tag="PK683_03045"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNH07452.1"
                     /translation="MKPFFIVIEGIDGSGKSTLSKSLFSELSRNMECCLFSEPTQFET
                     GLKIRKHLKGEISLSKADLISEFLEDRKLSIERNIQPSLSQGKNVILDRYFYSMAAYQ
                     SDSSTSAS"
     gene            complement(327..1172)
                     /locus_tag="PK683_03050"
     CDS             complement(327..1172)
                     /locus_tag="PK683_03050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH07453.1"
                     /translation="MKILAAAVTYNRKSLLERCILALKSQTYSDFEILVINNGSTDGT
                     SDWLAENGIPYITQENSGGAGGFYRAVEEGLAGKFDWIWCMDDDGYPDRSALSELINY
                     CSEDTACINSLVVSDQDSARICFHLPYVKNGNPVLKRPMKSLVELKNHSEDGRTVPFG
                     SFFNGTLISLDYVRKAGNVRKEFFIWGDEVEYAWRLMKFGKVITVLSALHFHPMTALS
                     VPDWKLYYGLRNAIYINNHTLNYSLIRNTRTVLKFLIKFIRIGNISLFFRALTDGFRG
                     RLGRL"
     gene            complement(1169..1699)
                     /gene="thpR"
                     /locus_tag="PK683_03055"
     CDS             complement(1169..1699)
                     /gene="thpR"
                     /locus_tag="PK683_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNH07454.1"
                     /translation="MRIFFAVFPPSEIQEQAERLQIGLPNTRWEPLENLHITLLFLGE
                     IPSSKLENIAESAHSLSLSQFFISFRSVRVFRNSRDSVLWTEAVPSDSLERLNLELKK
                     ISSSLGVIVQEKEFTPHMTLGRIKKFNESKFLEYAGLFCDFKTEEFSVNQFCLVSSVL
                     NPSGSKYRILETFSLA"
     gene            complement(1703..3301)
                     /locus_tag="PK683_03060"
     CDS             complement(1703..3301)
                     /locus_tag="PK683_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HNH07455.1"
                     /translation="MELEAKLESPQFQKHSSQINHLLIKMKTPPAVSTEQRQPLVIGL
                     AIDKSWSMKGDKIEATIEAASSLVNWLTRNDYVAIVAYSADVQVLQPFVQLKDKHAVV
                     EKIRSLQVGTSTNLSGGWLQTLKIVETSNIPNSYKRVILLTDGQATLGIKDQAQFEQI
                     AKDHFGRGISTTTIGFGNDFNEASLKAIADNGGGNFYFVSSPEEASGIFFKEFGDIGS
                     LYAQAVEVKIRFSEGVRMMDLFNEFPYTSESDGSVSIQAGDIRSDDIRSIVLALEINS
                     DKEINLKEVAKVGVTYYNLFDKTKLEKISYSISGEYSNNVIVDNEVVVERLIYSSAKT
                     IVKAARLLQDNQIEVAKTLVNSAIEKIEDHMALSPEVLSSVLGRLRNMSARLRENVHT
                     AGKHFMATGAEIFSRKEIIDTGGIETHDRIFEYKTVGDVDLYKCPDIKALVQNQIKDG
                     YKFIIFDLADTTFIDSSGIGAFIQIAGWLRRRGGELVVTNITDGVKRVFEVTRLENHI
                     RLAESMNEARDIIESIITSMQRTN"
     gene            3429..>3731
                     /locus_tag="PK683_03065"
     CDS             3429..>3731
                     /locus_tag="PK683_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNH07456.1"
                     /translation="MKVKILIIGSGPTGLGAGYRLKEKGETDFLILEKTDYIGGLATS
                     FTDSKGFTWDIGGHVQFSHYRYFDEVMIKALGKDGWLHHERESWVWMEDRFVPYPFQ"
BASE COUNT         1055 a          811 c          750 g         1115 t
ORIGIN      
        1 cgaagcagaa gtggaggaat cagactgata tgcagccatc gaataaaaat acctgtccag
       61 aatgacattt tttccttgag aaagtgaggg ttgaatattt ctttctatcg aaagtttcct
      121 gtcttccaaa aattctgaaa tcaagtctgc tttggaaaga gaaatttctc ccttcagatg
      181 ttttctgatt ttcagtccag tttcaaactg agtgggttcc gaaaaaagac agcattccat
      241 atttctggaa agctccgaga ataaagattt gctgagagtg gattttccgc tcccgtctat
      301 gccttcgatc acaataaaaa atggtttcat agtctgccta gcctgcccct gaacccgtct
      361 gtaagtgccc tgaaaaacaa ggaaatattt cctatccgga taaactttat caggaacttc
      421 aagactgttc tggtatttct gatgagagaa taatttaaag tatgattatt gatatatatt
      481 gcattgcgga gtccgtaata cagcttccag tcagggacag acaaggcagt cattggatga
      541 aaatgcagtg cagataaaac agtgatgacc ttcccgaatt tcatcaatct ccaggcatac
      601 tccacctcat ccccccagat aaaaaactcc tttcttacgt ttcctgcctt cctgacatag
      661 tccagactga tcagtgttcc attgaagaaa gatccaaatg gaactgttct tccgtcttca
      721 gagtgattct tcagttctac caaggatttc ataggacgtt tcagcacagg atttccattt
      781 ttcacataag gcagatgaaa acagatcctt gcactgtcct gatccgatac tacgagactg
      841 ttgatgcagg cagtatcttc tgagcagtaa tttataagct cagagagagc tgatctgtcc
      901 ggatacccgt cgtcgtccat acaccagatc cagtcaaatt ttcccgccaa tccctcttcg
      961 accgccctgt agaatcctcc cgcacctccg gaattttcct gtgtgatata aggtatcccg
     1021 ttttcagcaa gccagtcaga tgttccgtca gtgcttccgt tgttgatcac gagaatttca
     1081 aaatcactgt atgtctgact tttgagagcc agaatgcatc tttccaacag tgattttctg
     1141 ttgtatgtaa ccgctgcggc gagaattttc atgcaagcga gaatgtttcg agaattctgt
     1201 attttgatcc tgaaggattt aaaacagagg atacaagaca gaactgattt acggaaaact
     1261 cttccgtctt aaaatcacag aaaagccctg catattccag aaatttactc tcattgaatt
     1321 ttttgatcct gcctaaggtc atatgcgggg taaattcctt ttcctgaaca ataactccca
     1381 gagaagagga aatctttttc aactccagat tcagtctttc caaagaatcc gaggggaccg
     1441 cttccgtcca aagcaccgaa tctctgctgt ttcggaatac tcttacggac cgaaatgaaa
     1501 taaaaaactg acttagactt aatgaatggg ctgattcggc aatattttcc agttttgagg
     1561 aaggaatctc tcctaaaaaa aggagagtga tatgaagatt ttcgagaggc tcccatcttg
     1621 tattcggaag cccgatctgt aatctttccg cctgttcctg aatttcagaa ggcggaaaga
     1681 ccgcaaaaaa gattctcaaa tctcagtttg ttctctgcat tgaagtgata attgactcga
     1741 tgatatccct tgcttcattc atagactctg caagacggat atgattttcc agtctggtca
     1801 cttcaaatac tcttttgact ccgtcagtga tattggttac tacaagttct ccgccgcgtc
     1861 ttctgagcca gcctgctatc tggataaagg ctccgattcc ggaagaatca ataaaagtag
     1921 tgtctgcaag atcaaaaata atgaatttgt atccgtcttt gatctgattc tgaacaagtg
     1981 ccttgatgtc aggacattta tagagatcta catcgccgac agtcttgtat tcaaagattc
     2041 tgtcatgagt ttcaattccg ccggtatcaa taatttcttt tctgctgaaa atctctgcac
     2101 cggttgccat aaaatgcttt ccggcagtat ggacattttc tctgagcctt gcagacatat
     2161 ttctcagcct tccaagaact gaactcagaa cttccggaga aagagccata tggtcttcaa
     2221 ttttttcgat tgccgaattg acaagagtct tggcaacttc gatctgattg tcctgaagaa
     2281 gtcttgctgc ttttacaatt gtcttcgcag aggaataaat cagtctctct acgacaactt
     2341 cattgtccac gatgacatta tttgagtact cgcctgagat ggaataggat atcttttcaa
     2401 gcttagtctt atcaaagaga ttataatagg taactcctac ttttgccact tccttcagat
     2461 tgatttcttt gtctgaattg atttccagtg ccagaacaat gcttctgata tcatctgatc
     2521 ggatatctcc tgcctgaatg gatacagatc cgtcagattc tgaagtgtat ggaaattcat
     2581 taaaaagatc catcattctg acaccttcag aaaaacgaat ctttacctcc acagcctgcg
     2641 cataaaggga tccgatgtct ccaaattcct tgaagaaaat gccggatgct tcttcaggac
     2701 tgctgacgaa atagaaattt ccgccgccat tgtcggcgat cgccttgagt gaagcctcat
     2761 tgaaatcatt tccaaatccg atcgtagtag tggaaattcc tcttccaaaa tggtcttttg
     2821 cgatctgttc aaactgagcc tgatccttaa ttcccagagt cgcctgaccg tcagtcagca
     2881 gaatgaccct tttgtatgaa ttgggaatat tgctcgtttc tactattttt aatgtctgaa
     2941 gccatccgcc gctcagattc gtggaagtgc ctacttgcaa agatctgatt ttttccacga
     3001 ctgcatgttt gtccttcagc tgaacaaagg gctgaagaac ctgtacatct gcagaatacg
     3061 caactatggc aacatagtcg tttcgggtaa gccagttcac cagagaagaa gcagcttcta
     3121 tggtagcctc aattttgtcc cctttcattg accagctttt atcaattgca aggccgatga
     3181 ccagaggctg tctctgttcg gtcgaaacag cagggggagt tttcatctta atcagaagat
     3241 gattgatctg tgaactgtgt ttttgaaact ggggcgactc aagttttgct tctaattcca
     3301 tgcaggctag tatgaaaata aaataaattc ctgcaagatt atttcttttt cagtacgtaa
     3361 aaaaatgaga agtttttgtg ctccaaaaaa atacagacag tgtttttaag caatttttac
     3421 tggcagatat gaaagtcaaa attctgatta tcggatccgg tcctacaggc ttaggagcgg
     3481 gctacaggct gaaagaaaaa ggagaaacgg atttcctgat tctcgaaaag accgattata
     3541 tcggcggtct agcgacaagt ttcaccgaca gcaaaggatt tacctgggat ataggcgggc
     3601 atgtgcagtt ttcccattac aggtattttg atgaagtgat gattaaggct ctcggaaaag
     3661 acggatggct ccatcacgaa agagaatcct gggtctggat ggaagacaga ttcgtcccct
     3721 atccgtttca g
//
LOCUS       DAOQGJ010000129         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13874_length_3727_cov_3.289488, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000129 DAOQGJ010000000
VERSION     DAOQGJ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13874_length_3727_cov_3.289488"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..488
                     /gene="ssrA"
                     /locus_tag="PK683_03070"
     tmRNA           139..488
                     /gene="ssrA"
                     /locus_tag="PK683_03070"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(509..829)
                     /locus_tag="PK683_03075"
     CDS             complement(509..829)
                     /locus_tag="PK683_03075"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNH07457.1"
                     /translation="MKLLLDENLSYRILKKLDPDYQGSENIKNKTDQTIWEYAKNNGF
                     TIVTFDEDFYDIQLLRKFPPKVIWLRCGNTNTANIVGILNERKEEIRNFIEDSEQGIF
                     EIYE"
     gene            complement(851..1090)
                     /locus_tag="PK683_03080"
     CDS             complement(851..1090)
                     /locus_tag="PK683_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNH07458.1"
                     /translation="MNKKISIDYRLHIEINSEIRFGKPCIKSTRISVYDVLSWFASGM
                     TTDEILEDFPQLTKENILACFAYSADRERRLKIAV"
     gene            complement(1211..1573)
                     /locus_tag="PK683_03085"
     CDS             complement(1211..1573)
                     /locus_tag="PK683_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HNH07459.1"
                     /translation="MANKIWTYREASIALPKIILITEQTYKDYIEFNHDLKHRIWPEN
                     VQEEKEELIQILLADWEKSVEEIGAEVKGLWLVDFDNGKGYFCWKLGEDTLLFEHGYN
                     EGFSGRKPISRKIGDSGT"
     gene            complement(1563..3173)
                     /locus_tag="PK683_03090"
     CDS             complement(1563..3173)
                     /locus_tag="PK683_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNH07460.1"
                     /translation="MIAYWFVFRWAIRLLWHYKVYAGVFDKGKSLDKEEKLYTKLSLQ
                     TKVLFLKLGGVYIKAGQFLSNLAHIFPPQFIENLKDLQDRVPARSFPVMRERFFHEFG
                     KNIEEVFPDIDQVPLASASTAQVHKATLDGRKVAVKILYPGIEQLVHKDLKTIYFLMK
                     WINRYFFTFEYVKIHSEVKEMVLREMDLKKEGESISRMASFFRGQRDYVFPMVYDTHT
                     RRGVLVTKFIEGVKITEVKQQPGGKKSRPLDLLMRAYIQMIFKFHFFHADPHPGNVLY
                     TPQGKLCFLDFGAVGEVPSTISQSLKKIIISSTKKDYYSLIEAMGEMGFFDSSVNREK
                     MEQVAEYTLTRLNNFITNTDYFQNIGFDQLDSEDISLFLKGINTSLRDLMKIMQIPNN
                     YVMLERVFGILVGTAAILDPYRTVLEYSQPHFYELVEDKKDVWDTLEKEGSEIALAGL
                     SIPIEFQKALINLNRGRIRVINKELEKHTDKMFLLGHTAIYAFLTVSFIYIANQYSIQ
                     GQTSQSAVSYTVSAIFGISFLKKILYGK"
BASE COUNT         1080 a          730 c          720 g         1197 t
ORIGIN      
        1 acccgtcaga aggatgtaat tgcacttttt gaacatgggt gggaaagagc ggaaaaattt
       61 ttcaaaagaa aataaaatga agaaaaattg gattgcagga tatcgttttc taaaattcac
      121 agtctaatta gatgtaacgg ggacgacact ggtttcgact gttgtctgcc ttggtgcagt
      181 ggcatgtagg ggtgagagtc ctcttaaaaa ctttcacgcg aacaaacgca aacaacgaat
      241 acgctttagc agcttaatag ctgctttacg ggaaacagtc ttacacgtcc agtgggctgc
      301 gtaccgtcaa caaatggacg tctgaactgc ctggtttacg gcttggggcg gaacagtatt
      361 aaagtcgtta ggtgaagaac tccttgttgc gggtaaaact tctctgaaat tctcgtacac
      421 aacataaaca tgtagaggct gtattgacgc gcaataggac ccgggttcga ctcccggcgt
      481 ctccattttt tacaagcagc tcaagcaatc attcgtaaat ctcaaaaata ccctgctccg
      541 agtcttcaat aaaatttcgt atttcttctt ttctttcatt taaaattcct acaatgttgg
      601 ctgtattagt gtttccgcat ctgagccaaa ttactttagg aggaaatttt cggagaagct
      661 gaatatcata aaagtcttca tcaaaagtta cgatggtaaa tccgttattc tttgcatact
      721 cccatattgt ctgatcagtc ttgtttttaa tgttttcaga tccttggtaa tcgggatcta
      781 attttttcag tattcgatat gataagtttt catccaagag taatttcatt ttttatattc
      841 actgaaataa ctaaacagca attttcagtc tgcgttctct gtccgcagag taagcaaaac
      901 atgccagaat gttttcttta gtcaattgcg ggaaatcttc caaaatttca tccgttgtca
      961 tcccggaggc aaaccagctt aagacatcgt acacagatat tcttgtgctt ttaatgcacg
     1021 gtttgccgaa tctaatctca gaattgattt caatatgcaa tctataatca attgaaattt
     1081 ttttattcat atatcccctc cttgctatca tactgcttat ttttaaatta tctgcaagtc
     1141 tttttgactg acgggatcgt tttattattc ctaattatgt cctgagtgta tgaaagtaaa
     1201 aaaatgattt tcaggttcct gaatcgccga ttttccttga gattggtttc ctgccggaaa
     1261 aaccttcgtt gtagccgtgt tcgaaaagaa gggtgtcttc tcccagtttc cagcagaaat
     1321 atcctttgcc attgtcgaaa tcgacaagcc atagtccctt tacttcagca cctatctctt
     1381 ccacagattt ttcccagtct gccagaagaa tctgaatcag ttcttctttt tcttcctgaa
     1441 cgttttccgg ccagattcta tgcttcaggt catgattgaa ctcgatataa tccttgtagg
     1501 tctgttcagt aattaaaatg attttgggga gagcaatgga tgcttccctg tatgtccata
     1561 tcttatttgc catacagaat ttttttaaga aaggaaattc cgaaaatcgc tgagaccgta
     1621 taggagacag cagactgact tgtctgtccc tggatggaat actgatttgc tatataaata
     1681 aatgatactg taaggaaagc ataaattgca gtgtgtccca gaagaaacat tttatcagta
     1741 tgcttttcca gttctttatt gatgacacgg attcttcctc tgttgagatt gataagagct
     1801 ttctgaaatt ctatgggaat ggaaagaccg gcaagtgcaa tctccgatcc ttctttctcc
     1861 aaagtatccc atacatcttt tttatcttcc accagctcat aaaaatgggg ctgagaatat
     1921 tccagaaccg ttctgtacgg atcaaggatt gcagcagttc ccacaaggat gccgaagact
     1981 ctttccagca ttacatagtt gttcgggatc tgcataatct tcatcaggtc acgaagactt
     2041 gtattgattc ctttcaggaa cagtgaaata tcctcggagt ccagctgatc aaaacctatg
     2101 ttctgaaaat agtctgtgtt tgttatgaaa ttgttcagtc tggtcagtgt gtattctgcc
     2161 acctgttcca tcttttctct gttgactgaa gaatcaaaga agcccatttc tcccattgct
     2221 tcaatcagag aatagtagtc ttttttggta gatgatataa tgatcttttt cagtgactgc
     2281 gaaattgtag aaggcacttc tcccactgca ccaaaatcga ggaagcagag tttaccctga
     2341 ggagtgtaca gaacatttcc tggatgagga tctgcatgaa agaaatggaa tttgaaaatc
     2401 atctgtatgt aggcacgcat cagaagatcg agcggacggg atttttttcc gcctggctgc
     2461 tgcttgactt cagtgatttt cacgccttca atgaattttg tgacaagaac cccgcgtctg
     2521 gtatgagtat cgtataccat cgggaacaca tagtctctct gacctctgaa aaaagaggcc
     2581 attctgctga tggactctcc ttcctttttc agatccattt cacggagaac catttctttg
     2641 acttcagaat ggatttttac atattcgaaa gtgaagaagt atctgttgat ccatttcatc
     2701 aggaaataaa ttgttttcag atccttgtga acaagctgtt ctatgcccgg atagagaatt
     2761 ttgactgcca cttttcttcc atccagtgtt gccttgtgca cctgcgccgt agaggctgat
     2821 gcaagaggta cttggtcaat atctggaaac acctcttcaa tgtttttccc gaattcatgg
     2881 aaaaagcgct ctctcattac gggaaatgat ctggcgggaa ctctgtcctg aaggtctttc
     2941 agattttcaa tgaattgagg aggaaaaata tgggccagat tggataaaaa ctgccctgcc
     3001 tttatgtaca ctccgccgag cttaagaaaa agtactttgg tttgaagaga gagttttgta
     3061 taaagttttt cctctttgtc tagggattta cccttgtcaa atactccggc atatacctta
     3121 tagtgccaaa gaagccgaat tgcccagcga aaaacaaacc agtatgctat caaaaatcga
     3181 gcaaaatggc ttttcttttg gtataccaat gcttcagctg gaggtattat gtcacttttc
     3241 tgaaaattca tctctaagta ataccatttg tttgcgtaca agtccgaatt gcaaggagaa
     3301 aatacctgtt tttgctgaat ttagcccttc agatttgtga aaaagttttt gattcaaatc
     3361 aaaaaattaa aaatgacgca tctgtcgagg gaagtctttt ttgagaatag gaataagtat
     3421 aatcgtcaaa aaaattgccg tcagatcttt atcagggttc ctttttccca aaaaacctga
     3481 acctaatgtc aacttctgcc tgctgatccc tcaagaattc ataaaataaa gcaggatctg
     3541 caatagaaaa gactgaaccc tcttttgtgc agtagatttt ctgaagaaat attaagctga
     3601 tgacttggat acatataata gttttcaaaa ataattatat cttctcgaga ttcagacttg
     3661 tacttttttg tatagaataa aagttatgtg aaagattgga aaaacagata aaagatactt
     3721 gcatcat
//
LOCUS       DAOQGJ010000130         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13875_length_3727_cov_3.086329, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000130 DAOQGJ010000000
VERSION     DAOQGJ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13875_length_3727_cov_3.086329"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PK683_03095"
     CDS             complement(<1..1183)
                     /locus_tag="PK683_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNH07461.1"
                     /translation="MKYFRKFFPDSGFSWAVFGFLLLDLFMEKLPISTENLIWPLFFM
                     STISVFIFRIEKKFIISGLFLFVLYSEWTVRQNAERKHIVFSAKDWKTADRHWKSVNI
                     RFEKEIKKNFWEVKISHQNFEFPSILRHKTKSELIYDLECPLSAVKVNEADIKNEYLQ
                     FTKKYGSIYLSVRQKSCRRLEGRTDERRLVRKRTEELLEKGKIYDTARDISLGLIFGD
                     SGYLNDDLKKKAREGGILHLFAASGLHIGVLAAFLYFICRRIPFLDYYTEKMIPLALC
                     FAYLYLLNFPVSLLRAYLFASFFLVSQIFFRRMHKMDLILNSALFIRLYDPDSYLSLS
                     FLLSFSAVAGILLVKEDLDILLFGKHKSFFSENLTLSLSASLGTFPVLLYYFNSFSFG
                     SI"
     gene            1668..>3727
                     /locus_tag="PK683_03100"
     CDS             1668..>3727
                     /locus_tag="PK683_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07462.1"
                     /translation="MIPAEYPENESERIKKLKSYEILDTIDEVLYDDITKLASILCGT
                     PIALISFIDEKRQWFKSRVGLETKETHRDIAFCSYAILEHEIMEIPDSFKDERFFDNP
                     LAVSEPFVRFYAGAPLITPEGLTLGTLCVIDSRPKTLSSDQKTALTILSRNVVSLLEL
                     RLAQHHLNQDIERRKLLEDKLIKSEKRFSYSLDTMLQGCLIISYDYKYVYVNEAGADH
                     CRKKKEELLGCSLTEVFPEIESSPLFSGIKKCMEERIPLQLMNEFCFPDGSSAWFELN
                     IEPSPEGVFIVSSDVTGRREAEQRLLESEANLREAQSIANLGRWDLDHSTGKMKLSEK
                     VYDMFFIPKNNEEIYLDFLLTLVHPDDRKFAVEAYESSLKDRTVLDIEFRVLLPNEMI
                     RFVRLIGKNSYSTQNIPILSFGVIQDITDKKLDEENLRKAKEDADRANRAKSEFLADM
                     SHEIRTPMNAILGFAELLNEELRDDKKLSPLVRNITSSGKILLELINDVLDLSKIESG
                     VFRLVLAPINIRAILEDLRTVFSGKTAEKNIQFVLEVGKTVPQALLLDEIRLRQILLN
                     LAGNAIKFTDDGYIKISVMNTSQQRKDNSGAECTDLEIKVEDTGIGIPENQQESVFLA
                     FTQTEGQNHNKYGGTGLGLTIVKRLTNMMGGQISLKSQPGRGTVFTINIKDVETATAA
                     EVRTG"
BASE COUNT         1247 a          691 c          775 g         1014 t
ORIGIN      
        1 aaatcgatcc gaaactgaag ctgttgaaat aataaagcag aacgggaaag gtcccaaggc
       61 ttgcagaaag agaaagagtc agattttctg aaaagaatga tttatgcttt ccgaaaagca
      121 gaatatccag atcctccttg acaagaagaa ttcctgcaac agcggaaaat gaaagaagaa
      181 aggaaagtga cagataactg tccggatcat agagccgaat gaaaagcgcc gaattcagaa
      241 tcagatccat cttatgcatc cttcggaaaa aaatctgtga gaccagaaag aaagatgcaa
      301 acagatatgc acgcagaaga gaaacaggaa aattcagcag atacagatat gcaaaacata
      361 atgctaatgg aatcattttt tcagtataat agtccagaaa cggaattctt ctgcagataa
      421 aatacagaaa agcagcaagc actccaatat gaagacctga agcagcaaac agatgcagta
      481 tgcctccttc tctggccttt ttcttgagat catcgttcag atagccagaa tctccaaaga
      541 tcagtccaag agaaatgtcc cttgctgtgt cgtatatttt tcccttttcg agaagttctt
      601 cagtcctttt tctgaccagt cttctttcat cagtcctgcc ttccagtctt ctgcagcttt
      661 tctgacggac tgaaagataa atggagccgt atttttttgt gaactgaaga tattcatttt
      721 taatgtctgc ctcatttacc tttaccgcag acagaggaca ttccagatca tagatcagtt
      781 ctgattttgt cttatgcctg aggatggaag gaaactcaaa gttttgatga gaaattttta
      841 cctcccagaa attctttttg atctcttttt caaatcggat atttactgat ttccagtgcc
      901 tgtctgcagt tttccagtct tttgcagaaa acacaatgtg cttcctttcc gcattctgcc
      961 tgactgtcca ttccgaatac agaacaaaaa gaaagagtcc tgagataata aattttttct
     1021 caatcctgaa aatgaaaaca gatatggtgc tcataaaaaa cagaggccag attaaatttt
     1081 ccgtactgat cggaagtttt tccataaaca gatcaaggag cagaaatccg aatactgccc
     1141 aggaaaaacc tgagtctggg aaaaattttc tgaaatattt catacttgaa tagtatatct
     1201 gaatattatc agaagaaatt taattttttt atctcttcat tacaccttag aaagaataaa
     1261 tgcttcagga tgaatttctg tcatgtcgat ttcgaaaatt catttctgat tatagaattc
     1321 accttgcgca aaaaaagata cagactgagc tttagaggaa tctagcacta taaaagttta
     1381 tgatgaatca gtaaaaacaa cagctttcat gctttcggga gcttagccag cttctaaatg
     1441 ctgggggtca gcttccgaat ttgcgagtgc tcaatcctta tgcggtcatt gattagagcg
     1501 cccaatgtct atctgatgga agaataagaa tctcattgac aaagagtgat tcaaattgag
     1561 gcaatttttt ttaatcaaat attgacttta gatattaaaa tagcaaactg taatagagag
     1621 aaattgtatg aaacaattag aaaaagcggc gaaaggaaga aagtaggatg attcccgcag
     1681 aatatcccga aaatgaatca gaaagaataa aaaaactgaa aagttatgaa attctagata
     1741 ctatagatga agtattatat gatgacatta ctaaacttgc ttctattctc tgcggcactc
     1801 cgattgcact gatcagtttt atcgatgaaa aaaggcaatg gttcaagtcc agagtcggac
     1861 tggaaacaaa agaaacgcac cgtgatatcg ccttctgttc atatgcaata cttgagcacg
     1921 aaataatgga aataccggac tcttttaaag atgaacgttt ttttgacaat cctcttgccg
     1981 tatcagagcc cttcgtaagg ttttatgcgg gagcaccgct tatcactccg gaagggctta
     2041 cgcttggaac tctatgtgtc attgacagca gacccaaaac actgtcttcc gatcagaaaa
     2101 ccgcactcac cattttaagc aggaatgtcg tctctcttct ggaacttaga ctggctcagc
     2161 atcatctgaa tcaggatatt gaaagaagaa aattactgga agacaagctg ataaaaagtg
     2221 agaaacgctt cagttattct ctggatacaa tgcttcaggg ctgtcttatt atcagttatg
     2281 attataaata tgtctatgtc aatgaggcag gtgcagacca ttgcaggaaa aagaaggagg
     2341 aactccttgg ctgcagtctt actgaagttt ttcccgaaat agaatcctct cctttatttt
     2401 caggaattaa aaaatgtatg gaggaaagga ttccgcttca gctgatgaat gagttctgtt
     2461 ttcctgacgg ttcatctgcc tggtttgaac tcaatataga accttctcca gagggagtct
     2521 ttattgtttc ttcagatgtt accggcagaa gagaggcgga gcagagactt ttggaaagtg
     2581 aagcaaatct gagggaagca cagtcaattg caaatctggg aagatgggat ctggatcatt
     2641 ctacaggaaa aatgaagctg tcagaaaaag tatacgatat gtttttcatt cctaaaaata
     2701 atgaagaaat atatctggat tttcttctta ctttggtaca tcccgatgac agaaaatttg
     2761 cagtggaagc ctatgaaagt tcgctgaagg acaggactgt tttggacatt gagttcagag
     2821 tattgctccc gaatgagatg atcagattcg tcaggctgat tggaaaaaat tcatacagca
     2881 ctcagaatat tccgatcctt tctttcggtg tgatccagga cattacggac aagaaacttg
     2941 atgaggaaaa cctgcgcaag gcgaaggaag atgcggacag agcaaaccgc gcaaaaagcg
     3001 aattccttgc agatatgagt catgaaatac ggactccaat gaatgcaatt ttaggatttg
     3061 ctgaacttct gaatgaggaa cttcgagacg ataaaaaact cagtcctctg gtaagaaata
     3121 taacatccag cggaaaaatt ctgctcgaac tcatcaatga tgttctggat ctttcaaaaa
     3181 tagaatccgg agtattcagg ctcgtactgg ctcctatcaa tatcagagca attctggaag
     3241 atctgaggac tgtcttttcg ggaaagacag cagaaaaaaa tattcagttt gttctggaag
     3301 taggtaaaac cgtacctcag gcccttcttc tggacgagat cagactcagg cagattctcc
     3361 tcaatctggc gggaaacgca ataaaattta ctgatgacgg atatataaaa atttcagtaa
     3421 tgaacaccag tcagcaaaga aaggacaatt cgggtgcaga atgcactgat cttgaaatca
     3481 aagtggaaga tacaggaata ggaattcctg aaaatcagca ggaatcggtt tttctggcat
     3541 tcactcagac cgaaggacag aatcataata agtacggcgg gactggactt ggactcacaa
     3601 tagtaaaaag acttacaaat atgatggggg gtcagatctc tctgaagagt cagccaggca
     3661 gaggaacggt atttacaatt aacatcaagg atgtggagac tgccactgcc gctgaagtcc
     3721 ggacagg
//
LOCUS       DAOQGJ010000131         3720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13924_length_3720_cov_3.882401, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000131 DAOQGJ010000000
VERSION     DAOQGJ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13924_length_3720_cov_3.882401"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PK683_03105"
     CDS             <1..852
                     /locus_tag="PK683_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNH07463.1"
                     /translation="SFQMNSILKCFFFFFVLMLPVSAQDLDRSILGKILKKKELIVSV
                     AANYEPYYIKNPKPGYPGFEVEIAQKYAEFLDVKLTKVVPLNNFTEHAEAVIAGKIDL
                     ALGNSMSLVRAKQVYFSEPYVTVSIAGLVNKIILPQEQEGEVLLTKSYRSLLDIKNLA
                     RLNIGVKDKTSNADYMRSNFSQFPIQVFPSDTLGLAALNENKVNTYIADNLFLEGQIQ
                     KDPSLKSRYLPLLAPVIDKHLCIAFKKYDLQLQTSLNLFVREMKRTGEISRIKDRYFL
                     SNEWVVK"
     gene            complement(1028..2206)
                     /locus_tag="PK683_03110"
     CDS             complement(1028..2206)
                     /locus_tag="PK683_03110"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HNH07464.1"
                     /translation="MKKILILDDQLEMGIILKDTIEALGHSAKSVQTLREFILAYQGS
                     YDIFIIDLMLEDTDGIELLRYLELKNCSSQIILMSGAEPRTLYSAQQIGLNRGLNILG
                     TLHKPVKLDEIKALLSLSEKQPEQKQSVLINYEISRDDLENAVVNKEISIVFQPQIDI
                     RKNRVSGLEALSRWNHSVMGAIPPQAFIGAAERFSLIEELTVYVLQESLKTLNELNSS
                     YPETRMAVNFSALHFQDLQLPDKIQHLLAENKIPSELFTIEVTESNFFENYTDSMEIL
                     TRLSLKGINLSIDDYGTGYSNLQHLKNIPFNELKIDRSFVQDCSISSQVKMITENMVK
                     LGKELNMKIVAEGVEDENTLKLMREMDCDYVQGFFFSPPLTKIQLKNYLKDFDSPKKV
                     "
     gene            2618..2959
                     /locus_tag="PK683_03115"
     CDS             2618..2959
                     /locus_tag="PK683_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07465.1"
                     /translation="MIILKFISFLLLMMFMADCASPSKSSYRSEPSPGSAKEGVYSKI
                     VKGSGRAGKTAINKGSSALSEIQCTEAARMNAMASAYPGRTNFSGVTVIECRETSENF
                     ASCECDIGFRN"
     gene            3176..>3720
                     /locus_tag="PK683_03120"
     CDS             3176..>3720
                     /locus_tag="PK683_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07466.1"
                     /translation="MKMIKMIYAVFMLFFSINISAQQYNIANQADKEFDLIFYGQQNP
                     KMTRSGVPEFSYRSLKTVGSRAGLNKNQELSSAVYSGNFRKVQKLLSEGADVNAEAYP
                     YCGPKGHPIQLAVMSGSATMTRLMIEKGGDLKYTLHGGYTDISWGWLKASLTDISEEM
                     GRSDITDILKSKKGLKSSVRNV"
BASE COUNT         1088 a          737 c          775 g         1120 t
ORIGIN      
        1 agcttccaaa tgaattcaat tctaaaatgc tttttcttct ttttcgttct gatgcttccg
       61 gtttctgctc aggatctgga caggtcaatt cttggaaaga ttctgaaaaa aaaggaactt
      121 attgtttccg ttgccgcgaa ttatgaacct tattatatca aaaatcctaa acctggatat
      181 ccgggctttg aggtcgagat agcccaaaag tatgcagagt ttctggatgt aaaactgaca
      241 aaggtggttc ctttgaacaa cttcactgaa catgcggaag cggtcattgc cgggaaaata
      301 gatcttgcac tcggaaattc catgagtctt gtaagagcaa agcaggtata tttcagcgaa
      361 ccttatgtga cagtttccat tgcgggtctt gtgaacaaga ttattcttcc tcaggagcag
      421 gagggggaag tgcttctgac aaagtcttac agaagtcttc tggatatcaa aaatcttgcc
      481 agactcaata taggagtcaa ggacaagaca tcaaatgcag actatatgag aagcaatttc
      541 tctcagtttc cgattcaggt gtttccaagt gatactctcg ggcttgcagc tctcaatgag
      601 aataaggtaa atacttatat cgctgacaat cttttcctgg aaggacagat acagaaggat
      661 ccttctctga agtccagata tcttcctctt ctggcacctg tcattgacaa gcatctctgc
      721 attgcattca aaaaatacga tctccagctt cagaccagct tgaatctttt tgtgcgggag
      781 atgaaacgaa ccggtgaaat cagccgcatc aaggacagat attttctgtc caatgagtgg
      841 gtcgtgaaat agaatatttt tacttaaaat tctgtatttt ttcattaaaa aatgtctcac
      901 caagaacagt gagatttctc acagaatata attttgagca gcaagccttg ttcgaaaatt
      961 tattgactat aatttacctt cctgagttcg ttgaaggata ctgttgcgcg atctttacaa
     1021 tctcagttca gactttcttt ggagaatcga aatccttaag ataatttttc agctgtatct
     1081 tggtcagagg aggactgaag aagaatccct gaacgtaatc acagtccatc tctctcatca
     1141 gcttcagagt attctcatcc tccactcctt ctgcaacgat tttcatattc agttccttcc
     1201 ccagcttgac catattttcc gtgatcattt ttacctgaga gctgattgag cagtcctgaa
     1261 caaaggatct gtcgattttc aattcgttga acggaatgtt tttcagatgc tgcagattgg
     1321 aatatcctgt cccataatca tcaatggaca gattgattcc tttcagtgaa agtctggtca
     1381 ggatctccat tgaatcagta taattctcaa aaaaattgga ttcagtcact tctattgtga
     1441 acagttctga aggtatttta ttttctgcaa gcagatgctg aattttgtcg gggagctgca
     1501 gatcctgaaa atgaagagct gagaaattga cagccattct ggtttcagga taggaactgt
     1561 tcagttcatt cagtgttttc agactttcct gcaatacgta tactgtaagt tcctcaatca
     1621 gagagaacct ttcagcggca ccgatgaatg cctgaggagg aatggctccc attacgctgt
     1681 gattccatct tgacagagcc tccagaccgg aaaccctgtt tttccgaatg tctatctgag
     1741 gctgaaaaac aatggaaatc tctttattta ctacagcatt ttcaagatca tctctggata
     1801 tctcataatt tatcaaaaca ctttgtttct gttcaggctg tttttccgaa agtgaaagca
     1861 gagctttaat ttcatcgagt tttaccggtt tatgaagtgt ccccagaata ttcaggcctc
     1921 tgttcagccc aatctgctgt gcagaataca gagttcttgg ttctgccccg ctcatcagga
     1981 tgatctgact gctgcagttt ttgagttcaa gatatctgag aagttctatc ccgtctgtat
     2041 cttcgagcat aagatcaatg ataaaaatgt cgtaactgcc ctgatacgcc agtatgaatt
     2101 cgcgaagagt ctgaacagac ttggctgaat gtccaagagc ttcaattgtg tcttttagaa
     2161 tgattcccat ttccagctgg tcatccagaa tcagaatttt cttcataaac ccgtatcctt
     2221 ttgcacgctt ttctgcaggt tttcaggaat ctgatctgtt ttttcgaatc ttgaatactg
     2281 ccatctcata tttaacacga aaaaataaag tatgatatta aggacattct tattttaaaa
     2341 aaatacataa gcaggaatca gcattgtcct gagcttcaaa gctctttgcg catccatttc
     2401 ttcctgactc agcagaatga ctcttaaact tagcagcagt tttatctgca gatcctggat
     2461 aagacttcag tttatcctga gtattctgga caaagaattc atctgatttt tagtaagtat
     2521 tttataatat aataattatt attaattttc ccgaaatctt tttccctgcg cgaaaaaata
     2581 tattgactga accgacgaag catatgaatt tcaacgtatg ataattttga aattcatatc
     2641 ttttctgctt ttaatgatgt tcatggcaga ctgtgcatct ccgtccaagt caagctatcg
     2701 gtcagaaccg agtccaggtt ctgcaaagga aggcgtctat tccaaaatag tgaagggttc
     2761 aggcagggcc ggcaaaacag caatcaataa gggaagttct gcattgagtg aaatacagtg
     2821 cactgaggca gccaggatga atgcgatggc gtcggcctat cctggaagga caaatttttc
     2881 aggagtgacg gtcatagaat gcagggagac atcagaaaat tttgcatcct gtgaatgcga
     2941 catcgggttc aggaattagt catccgtttt caacggttta ttatgcaggc atttcgagaa
     3001 cagactgatt ctgtaacagt tatagaccgt caaaattttt acagtctaag aagacaatca
     3061 gtctaacaat catctgattt cagatgagca tcagtcatcc ggcaggcaat cagaatacag
     3121 cagctgtgag tcttttggct gagcagatgc agatcaagtg gacataggag atacaatgaa
     3181 aatgataaaa atgatttacg cggtgtttat gctgtttttc tccataaata tctcagcaca
     3241 gcagtacaat attgcaaacc aggctgataa agagtttgat ctgatttttt atgggcagca
     3301 gaatccaaag atgacccgtt ccggagtgcc tgaattttcc tacagaagtc tgaaaacagt
     3361 cggaagcaga gctgggctta acaagaatca ggagctttcg tctgcggtat acagcgggaa
     3421 tttccggaaa gtacagaaac ttctttctga aggggctgat gtaaatgctg aagcatatcc
     3481 gtactgcggt cccaaaggac atcctattca gctcgctgta atgtcaggtt ccgcgacaat
     3541 gaccagactg atgatagaga agggcggtga tcttaagtat acgcttcacg gagggtacac
     3601 ggatatttcc tgggggtggc tgaaagcaag tctgacggat atttccgaag aaatgggaag
     3661 atcggatatt acagatatac tgaaatcgaa gaaaggactg aagagttctg tccgaaacgt
//
LOCUS       DAOQGJ010000132         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13950_length_3716_cov_3.450697, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000132 DAOQGJ010000000
VERSION     DAOQGJ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13950_length_3716_cov_3.450697"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PK683_03125"
     CDS             <1..1094
                     /locus_tag="PK683_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07467.1"
                     /translation="FSILTFFHLLVISLAAYLYLSGTATQYAGIRFTETGSIFLTGVA
                     GIGFLVLAEKLGTWFSFLQKSSVMVGFQVVLLILLMIPLMEIKNHFWSRVLGKESHDH
                     SFFYDKTALNMADYLTQEQKKKKDSIVYWFDAGPVSFAGSEVLMRKVRYFEVYNYDYY
                     NYIKSHYWWKDVELDILNDSYLKNAVFAFPPFWRQKIITDYSGFSSPPTKEFGEYKIY
                     DTVNSSGAVFIGDAWNAPIKADAVSWGRYLKGREGGFVFWSVKEVSIAAEMNVIPTDK
                     DMAVGIFHEKKNIKLRYDLPKLMEKHKVRFKMKLSKGMNVLRISPISKTAAETQWGHI
                     KRSETPWLLFTEVNITKPEKEVEEGIYQM"
     gene            1308..2063
                     /locus_tag="PK683_03130"
     CDS             1308..2063
                     /locus_tag="PK683_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH07468.1"
                     /translation="MKISLIIPIYNEADHLKSFLSQIDSLALPAEKELVFVDDCSKDD
                     SFAILQSHSFSSSYRIIKHEKNQGKGAALRTGINEATGDVILVQDADFEYDMTELPGL
                     IRPILDGKADVVYGSRFRKESRQVHRTFHYLINRILTLLSNILSGLYVSDMETCYKIF
                     RSDIIKNINLQSNRFGFEPEVTAKVGRLKIRVFELPISYYPRNYIEGKKITWKDGVAA
                     VRHIIFFNLLQRKKNYFKKEMPEKYIPKDLNIL"
     gene            2262..2783
                     /locus_tag="PK683_03135"
     CDS             2262..2783
                     /locus_tag="PK683_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07469.1"
                     /translation="MKRSADQLNSVSSFFLLALLKIILFQLFSSDYPDRLFLPFLRRF
                     AENLTEGLFSDPWLPFAVAQPDSFPYHPMMLYAYGVFQIPAVFFPSLSKFLFILPSVF
                     ADLLIYTVLRRMFPAQRRAVLVLYGLSPVLLYSIYMHGQLDIWPTALLFYSLYLLVND
                     KIIKSSLFYGIAL"
     gene            2937..>3716
                     /locus_tag="PK683_03140"
     CDS             2937..>3716
                     /locus_tag="PK683_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07470.1"
                     /translation="MVLANSKQTLLLSFYYSIGTNQLYLAVSAILIIYLRFISYHKVN
                     KELLTAWLSVLFSVFVLLIPPAPGWYVWLLPFLMYFYLKFIRNRVQISGLYRLLIVSF
                     LMYFVFGWTGDYADLKFLGTVLDFKLFDAKTVNLFYTFLQVSLSVNLYVIYRKGVRSN
                     RIYTRPQSILIGIGGDSGAGKSTLLHSLERLLSPSVTLLEGDGDHRWERGSQNYEKIT
                     HLNPRANYLERQAENLIRLKKGETIYRPDYDHHTGKFTEPMP"
BASE COUNT         1047 a          716 c          760 g         1193 t
ORIGIN      
        1 agttttccat tctgaccttt tttcatctgc tggtcatttc ccttgctgcg tatctgtatc
       61 taagcggaac agctactcag tatgcaggca tacggtttac agaaacaggc tccatttttc
      121 tcacgggagt tgcaggcata ggttttcttg tgcttgcgga aaaactggga acttggtttt
      181 cctttctgca gaagagttct gtgatggtag gttttcaggt ggttctgctt attttgctca
      241 tgattcctct gatggaaatt aaaaatcatt tctggagcag agttctggga aaagaatctc
      301 atgatcattc cttcttttat gataagactg cactaaatat ggctgactat ctgactcagg
      361 aacagaagaa aaaaaaagac tccattgtat actggtttga tgctggccct gtttcttttg
      421 ccggatctga agttctgatg agaaaagttc ggtattttga ggtttataat tatgattatt
      481 ataattatat aaaatcacat tattggtgga aggatgtgga gctggacatt ttaaatgaca
      541 gctatctgaa aaatgctgta tttgcttttc cgcccttttg gagacagaaa attatcacag
      601 actattccgg tttcagttca ccgcctacca aagaattcgg tgaatacaag atttacgata
      661 cggtcaattc ttccggtgca gtgtttatag gtgatgcatg gaatgcaccg atcaaagcgg
      721 atgcagtttc atggggaaga tatttaaaag gaagggaagg cggatttgta ttctggtctg
      781 taaaagaagt aagtattgca gccgagatga atgtaattcc aacagacaag gatatggctg
      841 tgggtatatt tcatgaaaag aaaaatatta aacttcgtta tgatcttccg aaattaatgg
      901 aaaaacacaa agtcagattc aaaatgaaac tcagcaaagg tatgaatgta ttgaggatct
      961 ctcctatcag caaaactgct gcagagaccc aatggggaca tatcaagcgt tctgaaacac
     1021 cgtggcttct gtttactgag gtcaacatca cgaaacctga aaaagaagta gaggaaggca
     1081 tttaccagat gtagcatatg ctgtcttcct gcttgacagt aattatgcag atatgagatc
     1141 tgtcttctca tatccattga tgatgaaatg ggattaagca tattatgcat agggtaaacc
     1201 aaacagcatt tttcaaatct atgttcaaag gcatcagctt ggtccttttg cagaattacg
     1261 tctgttccga gtcagctcca tcgcgtaatt aaacttaaag aggccgaatg aaaatatcgc
     1321 tgattattcc aatttataac gaggcagatc atctgaaaag ttttctttcc cagattgatt
     1381 ctcttgcgct gcctgctgaa aaagagcttg tttttgtcga tgactgttcg aaagacgatt
     1441 ccttcgccat acttcaaagc cattcctttt ccagttcgta cagaattatt aagcatgaaa
     1501 agaatcaggg caagggtgca gctttaagaa caggcatcaa cgaggcgact ggggatgtca
     1561 tacttgttca ggatgctgat tttgaatatg atatgaccga acttcccggt ctcatcagac
     1621 caattctgga tggaaaggcg gacgttgtct atggctcgag attcagaaag gaatccagac
     1681 aggttcacag aacatttcat tatctgatca atcggattct gacactcctc agcaatatac
     1741 tttcaggact gtatgtgagt gatatggaaa cctgctacaa gatattcaga agcgatatta
     1801 taaagaatat aaaccttcag tccaacagat ttggatttga acctgaagtc actgcaaaag
     1861 tcggcagact gaaaataaga gtatttgaac tgcctatttc ctattatccc cgaaactata
     1921 ttgaaggaaa aaaaattaca tggaaagacg gtgtcgctgc tgtcaggcat atcatattct
     1981 tcaatctgct tcaaagaaag aaaaattact ttaagaaaga gatgcctgaa aaatacattc
     2041 cgaaggactt gaatattctc tgagttgaca gaatgactta ggttttataa tacactatta
     2101 tatgaagtct ttacagattt ctactctcta agtaagtacg cagctttaaa aaagctgaag
     2161 acagtgccga gaaagcatat agaaagaaat cttatcagga aaataagtct cttagaggag
     2221 gctgagtgtt tatacgccgg ttcattccga atgagttcat tttgaaaagg tcagcagatc
     2281 aactgaattc tgtcagttca ttttttctgc ttgcgctgct taaaataatt ctttttcaat
     2341 tattttcctc cgactaccct gaccgtcttt ttcttccatt cctgagaaga tttgctgaga
     2401 atctgactga gggactcttt tctgatcctt ggctgccatt cgcagtcgca cagccggatt
     2461 cttttccata tcatccgatg atgctctatg catatggagt atttcaaatt cctgcagttt
     2521 tttttccctc cctgtctaaa ttccttttta ttctcccttc cgtttttgct gatctgctca
     2581 tttatactgt tcttcggaga atgtttccgg ctcagaggag agcagtcctt gttctttatg
     2641 ggctttctcc ggtgcttctt tattccattt atatgcatgg tcagctggac atttggccta
     2701 cagcccttct tttttattca ttatatctct tagttaatga taaaataatt aagtcatcat
     2761 tattttacgg aattgcattg tgactcaaag ttcatattgc cgcagctctg cctctctttc
     2821 tgtattatgt ctggaggagg aagggtcata tatcagctgt acagtatgca gtttcgattt
     2881 ttctcactta ttctgctgtc ggtctgccct ggtacctcag ctcctccttt cgatctatgg
     2941 tacttgccaa ttcaaagcag actcttctgc tgagtttcta ttacagtata ggcacaaatc
     3001 agctgtatct tgccgtttca gcaatcctta tcatatatct caggtttatt tcctatcata
     3061 aggtaaacaa ggaactgctt acagcctggc tttctgtcct tttttctgta ttcgtccttc
     3121 tgattcctcc tgctccggga tggtatgtct ggctgcttcc tttcctgatg tatttttatc
     3181 tgaagttcat ccggaatcgg gtgcaaattt caggattata tagactttta attgtatcct
     3241 ttttaatgta ttttgtattt ggatggacag gtgattatgc tgatcttaag tttctgggaa
     3301 cagtgcttga tttcaagctc tttgatgcaa aaacagtaaa ccttttttat acatttctgc
     3361 aggtttctct ttcggtaaat ctgtatgtca tctacaggaa aggagtccgt tcaaacagaa
     3421 tctatacaag accgcagtcc atactgatcg gaataggagg ggacagcggt gcaggcaagt
     3481 ctacgctgct tcattcactg gaaagactgc tttctccttc agtgactttg ctggaaggtg
     3541 acggtgatca cagatgggag aggggaagtc agaactatga aaagattacc catctcaacc
     3601 ctcgtgcaaa ctatctggaa aggcaagcag aaaatctgat tcgtctgaaa aaaggggaaa
     3661 cgatataccg gccggattat gatcatcata ctggaaaatt cacagaacct atgcct
//
LOCUS       DAOQGJ010000133         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_13990_length_3710_cov_3.009850, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000133 DAOQGJ010000000
VERSION     DAOQGJ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13990_length_3710_cov_3.009850"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="PK683_03145"
     CDS             complement(<1..1629)
                     /locus_tag="PK683_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH07471.1"
                     /translation="MNESESEQNEVILLIEDDSGIGELIQEALSFTGRKTVHLDSGKS
                     ALEWIRNNDPFMIILDYSLPDMSGREVVENIISLKGRMPPFIVVTGAGDEKVAVSMMK
                     LGAKDYLIKDRSFLSHIPILAEQVIHEINVEYRLEKTQEALKESEKKFRQLFENMDEG
                     VVMILPDGIIIEANQAAEEILELKKNSIKKMWDVPTGWKIINAEGNEMLFEDLPGNIA
                     LLEKKPLKDQLMGILLPDGTVKWISVSFSPVFLDSGELNFLVGLVQNVTEQISIKKEL
                     EESEKRYHQLFDVSPLPAWVYSISSLKFLNVNTTALKVYGYSEEEFLNMSIKDIRPEE
                     DVQKLISFLSGVGEQDAYTNETRHLTKDGRIIDVMLWTNPILYKEEKARLVIVENITE
                     RKILEKELVKAKEAAEEASRAKSDFLAKMSHEIRTPLNGVMGMNSLLLTTNLDKEQRN
                     YVKTAYYSAESLLGVINDILDFSKIEARKLDIEEIHFSLPDMLNEFNTIVSMSAGEKG
                     LFFSTILDPEIPPEVIGDRNRIRQILLNLTTNAIK"
     gene            complement(1630..2109)
                     /locus_tag="PK683_03150"
     CDS             complement(1630..2109)
                     /locus_tag="PK683_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07472.1"
                     /translation="MIISNERKETVILITDDDEGHTELIHEHLVESGIINPILHFNDG
                     LAVWNFLSRKGDGPHMDDRHSYLLLLDIRMPKMDGVEVLRKIKNDPLLHSMPVIMLTT
                     TNDPKEIEECYEIGCNSYITKPVNYQQFGEAIKRLGLFISIMQIVPVNKGSRDGDGN"
     gene            complement(2093..>3710)
                     /locus_tag="PK683_03155"
     CDS             complement(2093..>3710)
                     /locus_tag="PK683_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07473.1"
                     /translation="IYDTVPVSIWETDWSIILKCIEKWKADGINDFEKFFSENSDETD
                     SLFSTVRLLDVNQWTLNLFEADSKNAVFSQFRKIFGTQEYRKIFLEQLQCIAEEKQVY
                     TSSVKFISFKGAPIDTILAASFPLKNSDSGLVFISIIDNTKYKNVLRELQEKEEELRQ
                     SEAIFHQMFTDHTAVKLLNSPETGRIIDANPAAEKFYGYSREDLLNMKMDQIAVFSEN
                     AEETGIVTERGYQKHRLSSGRHTDVEVYSAPIIVEGHYLIYSIIHDISDRLRAERERD
                     RLMFELETKNRELESVIYVTSHDLRSPLVNIQGFGENLNRYFTAISNALKSADVPADM
                     KVLLSEKIPKALHFIEMSSAKMDVLISGLLRLSRLGRVQLQFTQTDMNEVLKNILETR
                     AFQIESTYAKVEVQPDLPSCYTDKEQIYQVFSNLLDNAIKYKADDRVLTVTVTGKVLE
                     EKAVYAVSDNGIGIAEKDIGKIWEVFRRIESKSEVKGEGLGLAIVLRIVNRLNGKIWV
                     ESVPDSGSTFYMELPRENNTLSGEKNDYIK"
BASE COUNT          915 a          860 c          716 g         1219 t
ORIGIN      
        1 tttaattgca tttgtagtca ggttcagcag aatctgtctg attctgtttc tgtcgccgat
       61 cacttcagga ggaatttccg gatcaagtat tgtggaaaag aacagtcctt tttctccggc
      121 actcattgat actattgtat tgaattcatt cagcatatca ggaagactga aatggatttc
      181 ctctatgtcc agttttcttg cttcaatttt agaaaaatcc agtatgtcat taatgactcc
      241 gagaagggac tctgcgctgt aatatgcagt ttttacatag ttcctctgtt ccttgtccag
      301 gtttgtagta agcagaaggg aattcattcc catgactccg ttcagagggg ttctgatctc
      361 gtgactcatt ttggcaagga aatcactttt agcacggctg gcttcctctg ccgcttcctt
      421 cgccttgacc agttcctttt caagtatttt ccgttcagtg atgttttcca caatgacaag
      481 cctggctttt tcctccttgt acagaatcgg attggtccat agcattacat caatgatcct
      541 tccgtccttt gtcagatgtc ttgtctcgtt ggtatatgca tcctgttctc cgactccgga
      601 aagaaagctg atcagcttct gtacatcctc ctccggtctg atgtctttga tggacatatt
      661 caggaactct tcttctgaat agccgtaaac cttgagtgct gtcgtgttta cattcaggaa
      721 tttcagagaa ctgatggaat agacccaggc gggaagggga gatacatcaa agagctgatg
      781 atatcgcttt tcactttcct ccagttcctt tttgatgctg atctgttcgg taacattctg
      841 tacaagacct acgaggaaat tgagttctcc gctgtccagg aacactggag aaaagctgac
      901 cgatatccat ttgaccgtcc catccggaag caggattccc atcagctgat ctttcaaagg
      961 cttcttctca agcagtgcta tatttccggg aagatcctca aacagcatct catttccttc
     1021 cgcattgatg attttccatc cagtcggaac atcccacatt ttttttatgc tgtttttttt
     1081 aagctcaagt atctcttccg ctgcctggtt cgcctcgata attattccgt caggaagaat
     1141 cattacaact ccttcatcca tattctcaaa gagctgacga aatttcttct cgctttcttt
     1201 cagtgcctcc tgtgttttct caagtctgta ttctacattg atttcgtgta tgacctgttc
     1261 tgcaaggata ggaatatgtg aaagaaaact cctgtccttg atcagataat cttttgcacc
     1321 gagcttcatc atggatacag caactttttc gtctccggca ccggtcacta cgatgaatgg
     1381 aggcatcctg cctttcaggc tgatgatgtt ttctacaacc tccctgcctg acatatccgg
     1441 aagagaatag tccaggatta tcatgaacgg atcattattc cgtatccatt ccagagcaga
     1501 ctttccggaa tccagatgaa cagttttcct gccggtaaag gagagagcct cctgaatcag
     1561 ctctccgatt cctgaatcat cttctataag aagaatgact tcattctgtt ccgattcaga
     1621 ctcattcatt taatttccgt cgccgtctct gctgcctttg ttaacaggaa ctatctgcat
     1681 aatggaaatg aagagtccaa gacgcttgat tgcttctccg aactgctgat aattcaccgg
     1741 cttggtaatg taggaattgc acccgatttc atagcattct tcaatttcct tcggatcgtt
     1801 agtagttgta agcattatga ccggcatact gtgaagaaga ggatcgttct taatttttct
     1861 cagaacttcc acaccgtcca ttttcggcat cctgatatcc agaagcagaa gataggagtg
     1921 cctgtcatcc atatgcggtc catcaccttt tcttgagaga aaattccata cagcaagccc
     1981 gtcgttaaaa tgaagaatgg gatttattat gcctgattcc accaggtgct catgaatcag
     2041 ctctgtatga ccctcatcgt catcggtaat aagtatcact gtttctttgc gttcatttga
     2101 tataatcatt tttttcgccc gataaagtat tgttttctct gggaagctcc atataaaaag
     2161 tacttccgga atcaggaaca gattcaaccc agatttttcc gttcagacgg ttgacaattc
     2221 tgagcacgat tgccagtcca agtccctcgc ctttgacttc actttttgat tcgattctcc
     2281 tgaatacttc ccagattttg cctatgtctt tttctgctat tccgattccg ttgtctgaaa
     2341 ctgcatagac tgctttttct tccaagacct ttcctgtcac tgtgactgtg agaaccctgt
     2401 cgtccgcctt gtacttgatt gcattgtcca gcagattgct gaacacctga taaatctgtt
     2461 ccttgtcagt gtaacaggac ggcaggtcag gctgtacttc tacctttgca taagtgctct
     2521 caatctggaa tgcgcgtgtt tccagaatat tcttcagcac ctcattcata tccgtctgtg
     2581 tgaactggag ctgcaccctt cccagtctgg aaagacgaag aagaccgctg atcagtacat
     2641 ccatcttggc agaactcatt tcaataaaat ggagtgcttt ggggattttt tccgaaagaa
     2701 gcactttcat atctgcggga acatcagcag acttcaatgc atttgaaatt gccgtaaagt
     2761 accgattcag attttctccg aatccctgaa tgttcactag aggagacctc aggtcatgag
     2821 atgtaacgta aattacagat tccagttctc tgttctttgt ttccagttca aacatcagtc
     2881 tgtctctttc tctttccgct ctcagtctgt ccgagatgtc gtgaatgatt gaatatatca
     2941 gataatgccc ttccacaatg atcggtgccg agtaaacttc tacatctgta tgtctgccgg
     3001 aagaaagtct gtgtttctga taacctcttt cagtcacgat ccctgtttct tcggcatttt
     3061 cgctgaagac tgcaatctga tccattttca tgttcaaaag atcttctctg gaataaccgt
     3121 agaatttctc tgctgcagga ttcgcatcga tgattctgcc tgtttccgga gaattcaaaa
     3181 gttttactgc tgtgtgatct gtaaacatct gatggaaaat agcttcactc tgcctaagtt
     3241 cttcttcttt ttcctgcagt tccctcagta cattcttgta ttttgtattg tctataatac
     3301 ttataaaaac aagaccagag tcggaatttt tcaacgggaa ggatgctgcg agaatcgtgt
     3361 caatcggagc acctttaaat gaaatgaact tcactgaaga ggtatacact tgtttttctt
     3421 ctgcgatgca ctggagctgt tccaggaaga tctttctgta ttcctgtgtt ccgaaaattt
     3481 ttctgaactg agaaaaaact gcatttttgg agtctgcctc aaaaagattc agtgtccact
     3541 gatttacatc cagaagtctg acagtgctga acagactgtc cgtttcatct gaattttcag
     3601 aaaagaattt ttcaaaatcg tttatgccgt cagctttcca tttctcaatg catttcagta
     3661 tgattgacca gtcagtttcc cagatcgaaa cgggaactgt atcatatatc
//
LOCUS       DAOQGJ010000134         3691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14102_length_3691_cov_3.767327, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000134 DAOQGJ010000000
VERSION     DAOQGJ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14102_length_3691_cov_3.767327"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..720
                     /locus_tag="PK683_03160"
     CDS             280..720
                     /locus_tag="PK683_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07474.1"
                     /translation="MRIEKFTVKNNTTGNDRYASQFTAVWKKSQKESISEEFLAGGVR
                     EEYYSTGQYLFYFSWAVISGYSMTVIPFYRKEKSKLLLKSKSRNAEKVYDLEYTECFA
                     LLLLHCAFFEDFQPALMGLEERIENEIINQAVYDYLKNNEHFPK"
     gene            complement(924..2885)
                     /locus_tag="PK683_03165"
     CDS             complement(924..2885)
                     /locus_tag="PK683_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNH07475.1"
                     /translation="MKIFITVISIFFTLSGTLYSQGDSSEENEKNARAVKEIEESLKK
                     NQEELEALKKRLRQTDSSSSVKSGTEDSYVSPLKTGGLTPDYNRSMFIDADQSKFFNK
                     NQKPWLNDWIRAGAYIRTRYEDRFNLGFDKQAKGYISRALQTTQLFMIIDPSPYFSMK
                     INLQDARVFGGSTPASVGDVRANTFAGSGNTLTAGNSYTVPSQTTVREAWFMMKNLPL
                     DVKIQIGRQIPAYGDQRMLGGANWTINGLSYDGARLMIDKEYFNFHILGYKLTGNQSG
                     TNNVLSANAPIFSSTVDPATGKQLALNQGQPNQYIFGTYNTVKMKNWFHLDLYSLGLL
                     TARTPYYPTTATSLASVNANLGNYLYQSPWTKQKNNLITTGFRLTNRTAANALAQDSP
                     WKGWDWTFESAWQTGLTGTRVVSNSAVNAAANQFLSANGLPAGTFTNQNQKYAGAMYV
                     AQTGYTFFERTRIGLQYIYATGDNRSGDGTNGTFQTLPGPRMGTAPWNNMAGTSENID
                     MKNLKSTQISISHKTEKWGTFHASYFWNIKTQTNDAWYAVNGAANAGATTDYSSKAVT
                     GNRGRNIYNELDLTWMYGFHDYVSLWIGGGVLTAGNAVKGQKNAVVTYDPTVSGNING
                     YNINTSVARGASGAASQGYFMFFQFNALF"
     gene            complement(2953..3252)
                     /locus_tag="PK683_03170"
     CDS             complement(2953..3252)
                     /locus_tag="PK683_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07476.1"
                     /translation="MIKAFRVELIVANVVLQKTLEIMDSIGLNYTVFKDIAGRGERGS
                     KSAENVLTVLSCTYILSVCSQEKAARLTDSVSELLEIYGGTYFITETMAWDKTGL"
     gene            complement(3263..>3691)
                     /locus_tag="PK683_03175"
     CDS             complement(3263..>3691)
                     /locus_tag="PK683_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNH07477.1"
                     /translation="GALVVGYVTGEAGWKAEKPFTEELFKGFLSFFLLDMGLSAAKRF
                     KELKSVGFFLVFISFFLVFINATLGIILTKLVHMPVGDALMFTVLCASASYIAVPAAM
                     KMSIPQANPSIYLSVALSMVFPFNIIVGIPIYYYIISNLA"
BASE COUNT         1001 a          772 c          754 g         1164 t
ORIGIN      
        1 atataaaaac tttggataat ttccatgtct gacctttgcg ggtaagagtg tactgactta
       61 gtttttttct tcaagtacta atttaaaatt gtttctcaat ttgctcattt tgggacagta
      121 tgcaaaggct gtctcgagta aaatacaggg attatttatg cttccatata ttccgatact
      181 gttaaagtga gaattcttct tattttactt tttgtctttg ctgaatgccg tacaataaaa
      241 ctgaaatcgg tccagtctga tgaaaagtat aaaccagaaa tgagaatcga gaaattcact
      301 gtaaaaaata atacgacagg caatgaccgc tatgcaagtc aatttaccgc agtatggaaa
      361 aagtcacaga aggaaagtat ttctgaggaa tttctggcag gaggggtaag ggaggaatat
      421 tacagcactg gtcagtattt gttttacttt tcttgggcag taatttccgg gtattctatg
      481 acagtgattc cattttacag aaaagagaaa tcgaagctgc tgctaaagtc aaaaagcaga
      541 aatgcagaaa aggtttatga tctggaatat acagaatgct ttgcgctcct gctgctgcat
      601 tgtgctttct ttgaagattt tcagcctgca ctgatgggtt tggaggaaag gatagaaaat
      661 gaaattatca atcaagctgt gtatgattat ttaaagaata atgaacattt tcctaaataa
      721 tttatgcctg ctcttttgaa tcagtatctg ctatacaagg cagtactgga agtaccgtat
      781 gaacactcag catttatcaa tgtttcatta aaagataaaa ctgtttacgg aaatttttac
      841 ggattgatca tgaatctgga aaaagtattc atccaaaacc gtaattgctg tggatgaata
      901 cttcagctta tgtctgcatc tcttcagaat aatgcattga actggaagaa catgaagtac
      961 ccctgcgatg ccgctccgct tgcacccctc gcaactgatg tatttatatt atatccgttt
     1021 atatttccgg agactgtcgg atcataagtg acaactgcat ttttctgccc tttaacagca
     1081 tttcctgcag tcagcacccc tcctcctatc cagagagata cataatcatg aaatccgtac
     1141 atccaggtta aatccaattc attgtagatg tttcttcctc tgtttcctgt gactgctttg
     1201 gatgaataat ccgtggttgc tcctgcattt gcagcaccat ttacagcata ccaggcatcg
     1261 tttgtctggg tttttatatt ccagaagtaa gatgcgtgaa atgttcccca tttctctgtt
     1321 ttatgtgaaa tgctgatttg agtgcttttc aggtttttca tatctatgtt ttctgacgtt
     1381 cctgccatat tgttccaagg agcagttccc attctcggac cgggcagtgt ctgaaaggtt
     1441 ccgtttgttc cgtcgcctga ccggttatca ccggttgcat agatatactg cagaccgatg
     1501 cgtgtccttt caaaaaaggt atatcctgtc tgagcaacat acattgcacc tgcatatttc
     1561 tggttctggt ttgtaaatgt tccagcggga agtccgtttg ctgaaagaaa ctgatttgct
     1621 gcagcgttga ctgctgaatt gctcacaacc ctggttccag tgagtccagt ctgccaggct
     1681 gattcaaaag tccagtccca tcctttccag gggctgtcct gcgcaagcgc attcgcggct
     1741 gttctgtttg tcagtctgaa tccggtggta atcagattat tcttctgttt tgtccaggga
     1801 ctttggtaca gataattccc cagatttgca ttgactgagg ccagtgaagt tgcagttgtc
     1861 ggataatagg gagttctcgc agtcagaaga cccaatgaat acagatccag gtgaaaccag
     1921 tttttcattt tcactgtatt gtatgttccg aaaatatact ggtttggctg accctgattg
     1981 agtgccagct gtttccctgt ggcaggatcc actgttgaac tgaaaatagg cgcgtttgca
     2041 gaaagcacat tgtttgttcc tgactgattt cctgtcagtt tgtatcctaa tatatggaaa
     2101 ttaaaatatt ctttatctat catcagtctt gccccgtcat aggaaagacc gttgattgtc
     2161 cagtttgctc ctcccagcat tctctggtcc ccgtaggcgg gtatctgacg tccaatctga
     2221 atttttacat cgagaggaag gttcttcatc ataaaccagg cctctcggac agttgtctga
     2281 ctggggacag tataagaatt tcctgcagtc aatgtgtttc ctgatcctgc aaaagtattt
     2341 gctcgcacat cgccgacgct tgcaggtgtc gaccctccga agaccctggc atcctgaaga
     2401 ttaatcttca tactgaaata gggacttggg tcaatgatca taaacagctg agttgtctga
     2461 agtgctctgg aaatatatcc ctttgcctgc ttgtcgaaac ctaaattaaa tctgtcttca
     2521 tatcgggtcc tgatataggc tcctgcacgt atccagtcgt tcagccaggg cttctgattt
     2581 ttattgaaga actttgactg atctgcatca atgaacatag agcggttgta atccggtgtc
     2641 agtcctcccg tcttcagcgg agatacataa ctgtcttcgg tgccggattt tactgagctg
     2701 gatgaatctg tctgtctaag tctttttttc agcgcttcca gttcttcctg atttttcttc
     2761 aaagactctt caatttcttt tactgctctg gcattttttt cattttcctc actgctgtct
     2821 ccctgagaat ataaggttcc tgacaaagtg aaaaatattg aaataacagt gataaatatc
     2881 ttcattctaa tctcctattt ttaacgttcc tccgtccgct ctggaaaatg agcagccgga
     2941 gaagtatatt ttttacagac cagttttatc ccaggccatt gtttccgtga taaagtatgt
     3001 tcccccgtaa atctccagaa gttccgatac agaatctgtc agtctggctg ccttttcctg
     3061 agaacacaca gacaaaatat atgtacagga aagaacagtc agcacatttt ccgcactttt
     3121 ggagcctcgt tctccccttc ccgcaatatc cttgaataca gtataattaa gaccgatact
     3181 gtccattatt tcaagcgttt tctgcaggac tacattcgcg acaataagtt ccactctgaa
     3241 tgctttaatc ataatattat ccttatgcca gatttgaaat gatgtagtag taaattggaa
     3301 ttcctacaat aatgttgaaa ggaaaaacca tcgaaagagc cactgaaaga tagatgcttg
     3361 gatttgcctg gggtatggac attttcattg ctgctggaac tgcaatatag gatgcggaag
     3421 cacagagcac tgtaaacata agcgcgtctc ctacaggcat atgaaccagc ttggtcagta
     3481 taattcctaa agtcgcgttg ataaagacca ggaaaaaact gatgaagacc aggaaaaaac
     3541 ctactgattt cagttctttg aaccgttttg cagcagaaag tcccatatcc agaagaaaga
     3601 aggacagaaa tcctttgaaa agttcctcgg taaagggttt ttctgctttc cagccagctt
     3661 caccagtgac gtatccgacg accagtgcgc c
//
LOCUS       DAOQGJ010000135         3687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14131_length_3687_cov_3.376377, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000135 DAOQGJ010000000
VERSION     DAOQGJ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14131_length_3687_cov_3.376377"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PK683_03180"
     CDS             <1..1342
                     /locus_tag="PK683_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07478.1"
                     /translation="WLQRVSDQIAGAVQNAELLKEAEEAQKQVEFLNEFSKVINSAKD
                     LDTVFQQAVKELTEKLGTDIYILQLVDKKKNELFTRCYSASNSGNFNEIYYQWEIPLV
                     PESGSVYKTYSSKKTFYFKNNSRVSDERLSVFDRKWKNDLKLNATFQIPLMVQNEVIG
                     IMHINRYGGLQELQKEEIRFTESLCEQLAIAVNSSFLYELTEKEREKSEKLLLNILPE
                     DVAAELKEKGSTEPVMFDSVSVLFTDFKGFTKIAENMTPQELIKELDGCFSQFDRITE
                     RFHLEKLKTIGDSYMCAGGIPKTKKSNIPGAKNAVDAVLAALEIQSFMNQMKDIKMML
                     EVPYWELRLGIHSGPLVAGVIGEKKFAYDVWGDTVNTASRMESSGTPGMINISGSTYE
                     FVKDFFDCEYRGRINAKNKGEVEMYYVSRIRKGLSVNDSKNVPNGLFWERYEIL"
     gene            1606..2793
                     /locus_tag="PK683_03185"
     CDS             1606..2793
                     /locus_tag="PK683_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HNH07479.1"
                     /translation="MLFILLFFSSCAAKKGMKFKALFSIMSSDCTLSDLKISAGSLKE
                     TFSSSLTSYTASVPNSSGSTTVTATAANSGASITVNGAAVSSGTASGSISLAEGSNTV
                     TVKVTAQDASSRTYTITIVRVTNISTLSFLSVSSVTLYPSFSSAVTSYTASVQSSVSS
                     ITVTPTASDANASITVNGTATASGSASSSLSLNTGANTVTVSVTASDSAAVSVYTVTV
                     YRMVNNVYRFFITSSLYDGNLGGASGADSKCSSDANKPNDGSTYKAFVGTSGRYGCGT
                     TSDCTNPSENSDWVLKPNKNYARTDGTALFTSNAAGIFLSSGSMSNSFESGVQKAYWG
                     GFSSGIIWNYSTTNCSDWTSSSSGNQGRIGLSDSTGYYNAVRDLTTTPNCNTMQYLLC
                     AEQ"
     gene            2842..>3687
                     /gene="purB"
                     /locus_tag="PK683_03190"
     CDS             2842..>3687
                     /gene="purB"
                     /locus_tag="PK683_03190"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNH07480.1"
                     /translation="MIDRYSNPEIAAIWSLENKFEIWKDIEILACEVRMNRGEIPKED
                     FEEIKAKAKFDVPGILELEKTLQHDVIAFLTNMGNYIGPASRHVHYGLTSSDIGDTAL
                     CVQMSQAMDLILKRLDELIASVKEKAEEHKMTPCIGRSHGIHAEPMTLGLKFALFYEE
                     LKRNRERMLLAKKEISVGKFSGAVGTFSNIDPEIEEFVCSRLGLEPDPITTQVITRDR
                     HAFYASVLGICAGTLDRFATEIRLLQKTEGREVEEPFGKGQKGSSAMPHKRNPVICER
                     ISGISR"
BASE COUNT         1087 a          734 c          878 g          988 t
ORIGIN      
        1 ctggcttcag agagtctcgg atcagattgc aggtgccgtt cagaatgcag agcttctgaa
       61 ggaagcggaa gaggctcaga agcaggtgga atttctgaat gagttttcca aagtgatcaa
      121 ttcggccaag gatctggaca ctgtttttca gcaggcagtc aaggagctga ctgagaagct
      181 cggaacggac atatatattc ttcagctggt ggataaaaag aaaaatgaac tctttacgag
      241 atgttattct gcatcaaatt caggaaattt taatgagata tactatcagt gggaaattcc
      301 gcttgttcct gaatcaggaa gtgtatataa aacatattca tcaaaaaaga cattctattt
      361 taaaaacaac agcagagttt ctgatgagag gctttccgta tttgacagga aatggaagaa
      421 tgatctgaag ttgaatgcaa catttcagat tcctcttatg gtgcagaatg aagtgatagg
      481 cataatgcat atcaacaggt acggaggact gcaggaactg cagaaggaag aaatccgttt
      541 tactgaatca ctctgtgaac agcttgcaat tgcagtgaac agctcgtttc tgtatgagct
      601 tacggaaaag gaaagagaaa aatcggaaaa actcctttta aatattcttc ctgaagatgt
      661 tgccgcagag ctgaaggaga agggctccac tgaaccggta atgtttgatt ccgtctctgt
      721 gctgtttact gatttcaagg gtttcacaaa gattgcggaa aatatgacac ctcaggagct
      781 gattaaggag cttgacggat gtttctctca gttcgacaga atcactgaga gatttcatct
      841 ggaaaagctg aagactatcg gagacagcta tatgtgtgcc ggcgggatac ctaagacaaa
      901 aaaatcaaat attcccggtg caaaaaatgc agtggatgca gttcttgcag ctctggagat
      961 ccagtcattt atgaaccaga tgaaggacat caagatgatg cttgaggttc cgtactggga
     1021 acttagactc ggaattcaca gcgggccttt agttgcagga gtgatcgggg aaaagaagtt
     1081 tgcttacgat gtatggggcg acacagtgaa tacagcaagt cgaatggaat ccagcgggac
     1141 ccccgggatg atcaatataa gcggaagtac atatgaattc gtgaaggact tttttgactg
     1201 cgagtacaga ggcagaatca atgcaaagaa caagggtgag gttgaaatgt actatgtcag
     1261 cagaattcgg aaagggttat cagtgaatga ttcgaaaaat gttcctaacg gacttttttg
     1321 ggaaagatac gagattctgt aatattcaag ttttctaata ttgcaggagc gcaatgcctt
     1381 aaattctggg tttaagcagc ttcatttatc tgaagtctta cccggacaga atttcaaatc
     1441 tgtgaaaaat tgaaatttta ggaggtttct aaaaaaatta ttttgagatt cgaaaaaaca
     1501 gaatgttttc tctaaatgaa gcactcatcc attttttacc gataatttct gcttgaaaat
     1561 cctgtcttat ccgtcaaata ttaagctatg ttccggaagc tgccaatgct tttcattctg
     1621 cttttttttt cgtcctgtgc tgccaagaag gggatgaaat tcaaggcact gttcagcatt
     1681 atgtcttcag actgtaccct ttcggattta aaaatcagtg ccggaagcct gaaggaaact
     1741 ttttcttcgt ctctcacgtc ctatactgct tccgtcccga attcttctgg ctcaactact
     1801 gtcaccgcaa ctgctgcaaa ttccggtgca tccattactg tgaatggagc agcagtgagt
     1861 tccggcactg cctcaggaag catcagtctt gcagaaggct caaatacagt cacagtcaaa
     1921 gtgactgctc aggatgcatc ctctagaaca tatacaataa ctatagtaag agttacaaat
     1981 ataagcactc ttagttttct ctcggtcagc agtgtcactc tttatccctc attttcttct
     2041 gcggtgacat cttatactgc ttctgtccag tcttctgttt catcaattac cgtaacgccg
     2101 actgcatcgg atgcaaatgc ctccatcact gtgaacggga cggcgactgc ttccggctca
     2161 gcttcttctt cgctcagtct gaatacggga gcgaatacag tgactgtttc agtgacagca
     2221 tcggactctg cagctgtatc agtgtataca gtcactgtgt accggatggt aaataatgtt
     2281 tacagattct tcataacatc atcactgtat gacggcaatc ttggcggggc ttccggtgcg
     2341 gactcgaaat gcagttctga tgcaaacaag ccgaatgacg ggagcacata caaggctttt
     2401 gtcggaacct caggacgtta cggatgcgga acgacgagcg actgcacgaa tccttctgag
     2461 aattcagact gggtgctcaa gccgaataag aattatgcca gaacagacgg aactgcactg
     2521 tttacatcca atgctgcggg gattttcctt tcttcaggtt ccatgtcaaa ttcatttgag
     2581 tccggggttc agaaggctta ctggggaggt ttttcttccg gaattatctg gaattattcc
     2641 actacgaact gttctgactg gacaagcagc tcttccggaa atcagggaag aataggactg
     2701 agtgattcaa ccggatatta caatgcagtc cgtgatctga ccacaactcc gaactgcaat
     2761 acgatgcagt atcttctctg tgcggagcag taaaaaaacc ttccttgacg ttcatctgcc
     2821 ctgatctgaa ctggaatcag tatgatagac agatattcca accctgagat tgcagcaatc
     2881 tggagtctcg aaaacaaatt tgagatatgg aaggatattg aaatccttgc ctgtgaagtc
     2941 agaatgaaca gaggtgaaat tcccaaagag gatttcgagg aaattaaggc caaggctaaa
     3001 tttgatgttc cagggattct ggaactggaa aaaacacttc agcacgatgt gattgcattt
     3061 ctgacgaata tgggaaatta tatcggaccg gcttcgaggc acgtgcatta cggtctgaca
     3121 tcttcggata taggagacac tgcactctgc gttcagatgt ctcaggcgat ggatctgatt
     3181 ctgaaacggc ttgatgaact tatcgcctca gtaaaggaaa aagcagaaga gcacaagatg
     3241 actccatgca tcggacgttc acacgggatt catgcggaac cgatgactct tgggctgaag
     3301 tttgcactgt tttatgaaga gctgaaaaga aacagggaaa gaatgcttct tgcaaagaag
     3361 gaaatttccg taggtaagtt ttcgggagct gtgggcacgt tttccaatat tgatcctgag
     3421 atagaagaat ttgtgtgcag cagactcggt ctggaacctg atccgatcac aactcaggtc
     3481 atcacaagag acagacatgc attttatgca agtgtcctcg gaatctgtgc agggactctt
     3541 gacagattcg ctacagagat ccgtcttctg cagaagacag agggaagaga agtggaagag
     3601 cctttcggaa aaggacagaa gggctcttcg gctatgcctc acaagagaaa tccggtcatc
     3661 tgtgaaagga tatccggtat ctcaaga
//
LOCUS       DAOQGJ010000136         3668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14242_length_3668_cov_2.898976, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000136 DAOQGJ010000000
VERSION     DAOQGJ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14242_length_3668_cov_2.898976"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..2883
                     /locus_tag="PK683_03195"
     CDS             1..2883
                     /locus_tag="PK683_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07481.1"
                     /translation="MILVANYIKPQVTKIFIFSYFISVQEREILTESYRKQTESARSS
                     IQSAVSEIDTVSENLIQIEIFRNHISAKKKELQKYQTVQYYTKEKEYNFLGMKIKAGG
                     MLGNIVGKETFSVKADTYFSQYYNSEDAAQLEGRIRVQLSYSIGRPVTDDEWKRLKAF
                     AAECSQAENEIQKPADSRKEDSNQLKGKKAEAENKYTQSKAALNKEITKIYIQQQKRK
                     LEEMGLNTELIRIQIFEKTRNNDISLSFDSASIDPFSGFSKLNYKEFPAFETSVARSF
                     QRFGASAYSDAKDADTVSWKEKEGTKRTIHTVYSPHFRNQNSTAHAQEFMSFQKLSGF
                     GSYIEDDRQHVKRLTGHIEKLRKRLGELKKTVKVRRSAESQNGNPERKEGSSHSQKSG
                     LKDHNSKFIEEEIAAVAVVPEKDSEFMRLYNEYKYELLSRYKSYNSLKTGLSEKISKK
                     DPGAGYVLRQLESMDSMRDSALEDRIVLRHKTNDSELSLFYTDSRKNAEMRARWKMIR
                     DWIMEGHSEMPSPELKKLFPDGTIIKSRSEAEEIMWEMDSLPFFAEDGIPALSQRILQ
                     SNFSGVVQTLIDGTEGEELIQKNIFQLLYRALIICTFSVLLAFYISGIAVQKIKRIIG
                     SAEEVGKGNLNVEFEHAGNDEFGHLTVSLNQMVTGLREREKMRTVLGSMIDPVVVGEA
                     MKDLAALKRGSEKNITAFFSDIAGFTGISEKLKPFELAALLNEYLSAMTIILKKHDGV
                     LDKYIGDAIVGIFNAPLDVNDHCLKAVTASLEMQKKLTEMKQAWKKENKYIPEVHEMH
                     FRIGLNTGSAKVGFMGTDALAAYTMMGDTVNLAARLEAAGKDYGVWNLIPSNVYDLVK
                     DKVFARRLDYVRVKGKNEPVVLYEVISMKNETEEKMKKFVSQYEDGLQYYLNRNWKEA
                     IEKFESAEKTKGEKDKACGILTERCQEYLNSPPPVDWDGVFTRTHK"
BASE COUNT         1276 a          616 c          828 g          948 t
ORIGIN      
        1 ataattttag tagcaaatta tataaaacct caagttacaa aaatatttat tttttcttat
       61 tttatttcag tgcaggaaag agaaattctg actgaaagct acagaaagca gacagagtct
      121 gcccgttctt ccattcaaag tgcagtcagc gaaatagaca ctgtctctga aaatctgatt
      181 cagatagaaa tcttcagaaa tcatatttca gcaaaaaaga aggagcttca gaagtatcag
      241 accgtacagt attatacaaa ggaaaaagaa tataattttt tagggatgaa aataaaggct
      301 gggggaatgc tggggaatat agtaggcaag gaaacctttt cagtaaaggc agatacatat
      361 ttttctcagt actacaattc agaagatgct gctcagctgg aaggaagaat acgagttcag
      421 ctgagttact caattggccg tccagtcact gatgatgaat ggaagagact taaagcattt
      481 gcggctgaat gttcccaggc tgagaatgag atccagaagc ctgcagacag caggaaagaa
      541 gactcaaatc agcttaaagg aaaaaaagct gaggcggaga ataaatacac tcaatcgaaa
      601 gcggccctaa ataaagaaat tacaaaaatc tatattcagc agcagaaaag aaagcttgaa
      661 gagatgggac tgaatacaga actgatccga attcagatat ttgagaagac aagaaataat
      721 gatatttcct tatcttttga ttctgcctcc attgatccgt tttccggatt ctccaagctt
      781 aattataagg agtttcctgc ttttgaaacg tctgttgcca gatcattcca aagattcgga
      841 gcgtccgctt attctgatgc aaaagatgcg gatacggtat cctggaagga aaaggaagga
      901 acaaaaagga caattcatac agtctactct cctcatttca gaaatcagaa ctctaccgcc
      961 catgctcagg aatttatgtc ctttcagaag ctttccggtt tcggaagtta tatagaagat
     1021 gacagacagc acgtaaaacg tctgacgggg cacattgaaa agctgagaaa acgtcttgga
     1081 gaactgaaaa aaactgtcaa agtaagaagg tctgctgaat cacagaacgg gaatccggag
     1141 aggaaagaag gaagcagtca ttctcagaaa tccggattga aagatcataa ttcaaagttc
     1201 attgaagaag agattgctgc cgttgcggtt gttccggaaa aggattctga atttatgcgc
     1261 ctgtacaacg aatataaata tgaattatta tccagatata aatcatataa ttcattaaaa
     1321 accggcctgt cggaaaaaat ttcaaagaag gatccaggtg cagggtatgt tctgcgccag
     1381 cttgaatcca tggacagtat gagagattct gccttggaag acagaattgt tctgcggcac
     1441 aagacaaatg attctgagct ctcactcttt tatactgatt ccagaaaaaa tgcagaaatg
     1501 agggcacgct ggaaaatgat ccgtgactgg attatggaag ggcactctga gatgccctct
     1561 cctgaattaa agaaactgtt tcctgacgga acaattatca aatccagatc tgaagcggaa
     1621 gagattatgt gggagatgga cagtcttcct ttttttgcag aggatggaat ccctgcattg
     1681 agtcagagaa tacttcagag caatttttcc ggggtggttc agacacttat agacggaaca
     1741 gaaggtgaag agcttattca gaaaaatata tttcagttat tatatagagc attaattatc
     1801 tgtacttttt cagtacttct tgctttttat atttctggca tagccgttca gaaaatcaag
     1861 agaatcatcg gaagtgcaga ggaagtaggg aaaggaaatc tgaatgtaga atttgaacat
     1921 gcgggcaatg acgagttcgg tcatctgaca gtctccctga accagatggt gacaggactc
     1981 agagaaagag aaaaaatgag gacagtgctt ggaagcatga ttgatcctgt ggttgtaggt
     2041 gaagcaatga aggatctggc ggcactgaag cggggaagtg aaaaaaacat cactgcattt
     2101 ttttccgata ttgcaggatt tacaggcatt tccgaaaaac tcaaaccttt cgaacttgca
     2161 gccctcctga atgaatatct ttctgcaatg acaattatat tgaaaaaaca tgatggtgta
     2221 ttggataagt atatcggtga tgcaattgtg ggaatattca atgcgcctct ggatgtaaac
     2281 gaccattgtc tgaaagcagt tacagcaagt ctggaaatgc agaagaaact gactgaaatg
     2341 aagcaggcct ggaaaaagga gaacaagtac atcccggaag tgcacgaaat gcattttcgg
     2401 atcggactga atacaggatc agctaaagtg gggtttatgg gaacagatgc actcgctgca
     2461 tatacgatga tgggagatac agtcaacctt gcagcaagac tggaagccgc aggaaaagac
     2521 tatggtgtat ggaatcttat tccatcgaat gtatatgatt tggtgaaaga caaggtattt
     2581 gccagacggc tcgattatgt gcgtgtaaaa ggaaaaaatg aaccggtcgt actgtatgaa
     2641 gttatcagta tgaaaaatga aacagaagag aaaatgaaga agtttgtatc acagtatgaa
     2701 gatggacttc agtattattt aaaccgtaat tggaaagagg ccattgaaaa atttgaatcg
     2761 gcagagaaaa caaaaggaga gaaagataaa gcttgcggaa tactgacaga aagatgtcag
     2821 gagtatctga attctccgcc gcctgtggac tgggacggag tatttaccag aacacataaa
     2881 tagacggtat ggccgaaagt ctggcagttg cagaattttt tattgcgggt tttcgttcct
     2941 tagttcagca tcggaaaagg gtgtctattt tctgtctcgt cccagaagat tcggcaaaag
     3001 tcttccgatt tcctctggaa gaaatatttt caggaaataa agaactctta aaggggcttt
     3061 ggatttatca atccgattac agttgggaaa aacatcctgt cattcatctg catctcagca
     3121 aacagagatg cggagacacc tgatgctctg agagctatca ttgcaaatta attagaatat
     3181 atagcaaaag aatattcagt ttctctggaa aaatttcatt agaaatctgg gtatcataaa
     3241 cagtctgagg agctggtatt aaaatcgcac agaataaaca agacagtcat tctcattgat
     3301 gcatacgaca aatcaatcat tgactggctg gataaaacgg cacttgccaa agaaatgcgc
     3361 tctgttctga aatgttttcc ggattatata aaagcatttg ctgaaaaatg agaaaatatc
     3421 ggaagaagcg ctaaggaaga accctttgct cagatcaaag cagaaaagta ttatgaaaag
     3481 tataggaacc aaggcaaacc gatttttttg atcggagcag agtttaagga tcggaacatt
     3541 ggggattata ctgttgaaac tctaatgatg cagggatgaa tctgaaaaat tcaaaaaatt
     3601 aatttgctct gaacttgact tttccgacaa ataaaataaa cctcaaagga cactctcaat
     3661 gaaatctt
//
LOCUS       DAOQGJ010000137         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14349_length_3652_cov_4.072838, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000137 DAOQGJ010000000
VERSION     DAOQGJ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14349_length_3652_cov_4.072838"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2022
                     /locus_tag="PK683_03200"
     CDS             <1..2022
                     /locus_tag="PK683_03200"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07482.1"
                     /translation="PGEKAKAGFLDLSSVNFFTEIVNLDGEWEFYWNELIASEKKNPE
                     TLSKILINVPDAWNGTEIGKEKLSGQGFASYRLKFKVKKTDTPFALKALDMATSYSIW
                     FNGEKIWWNGKVAKTKAESVPEFKPNTVFLSNVKEENEIIIEVSNYHHFKGGFWESLK
                     IGRLENLILFREKRFGFDLFLTGSLLIMGIYHFGLFSLRRKDKSTLLLGIFCFIMLFR
                     LLTTGERIFFVVFPFLGWDFWLRIEYLSFYLATPVFASFLHSLYPYEFKRRATQIAIL
                     GSSVFCLFVIFGTAKFFSSTPIPFQIFTVIFLIYGFIQLTRASIRKREGGILALIGFI
                     MLLLTTVNDILYGNFIINTTYLSPYGFFAFIFTQAFILSVRFSKAFLNVELLTEDLQK
                     TNHAYSRFVPKEFLSYLQKNNIMDVTPGDQVKKEMTILFSDIRSFTNISENMTPEENF
                     DFVNSYLAVMNPIIKKNRGFVDKFIGDAIMALFPDSPEDAINAGIEMQKGLKEFNIRR
                     LQEHKELIQVGIGIHTGSLMLGTVGDSDRMEGTVISDAVNLASRIEGITKVYGANIII
                     SEQTLTALPDTSQFALRFLGKVEVKGKKDPATVFEIYEADDADVREKKIESQIMFEKG
                     IHDFLTGNPHNAKLLFEEILERNPKDLPAYKYLQKCSLIDSVQSANADY"
     gene            complement(2016..>3652)
                     /locus_tag="PK683_03205"
     CDS             complement(2016..>3652)
                     /locus_tag="PK683_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07483.1"
                     /translation="DTPTWPVLRNLIQAAVLGIWGLNTDYDSLITVVQFGLMIFCVLL
                     ILWKDFHFAGLLFSVLLFFSLLSASDFIIYIFSPMLEIQGALFFLLSVHFSYICVRRK
                     SPSHSELLKAGISIFLLLQTKYPYGYMFLFCLFLFLFFSETSYSAFFFIRYLLYSLYR
                     KSGLFSFSAVLLILFLLSKGHISSRLSKNIFYVLLISGTADFVLYIIREKASLFNLGY
                     EKIINIFKYLFLPSVLWVISHPDRLGSSAGTISHVQMEGYGVGEAVEKNLDYYLYFFK
                     TVFFSFWDRPYSIIISGTFLFALTLFLFLAFRKGFRHVSRFQYFFFFLFAGILGLSVL
                     TPNHQKRHILHLLPLWPFIALYGFRDACFIIIKYISALITRTEELKFFSYILQSNARK
                     YSVLLFQTVCLGTALFFMHDQMTKIPLRLQNVNLCFSGKQNIYETPRELKTLAADLVR
                     ENTVFLSTVEDWHYNKPDTELVIRILTFKNRVKLSFRAEDFQKKKNAFSQILIGGKNC
                     DVSAESLQKLAGTELVLTQKKSGKFSCAAVYKRKSQ"
BASE COUNT         1199 a          735 c          751 g          967 t
ORIGIN      
        1 cctggagaaa aagcaaaggc aggtttcctt gacctcagtt cagtcaattt ttttactgaa
       61 atcgtcaacc tggatgggga atgggaattt tactggaatg agctgatcgc ttctgagaaa
      121 aaaaatccgg aaactctttc caaaatactg atcaatgttc ccgatgcctg gaacggaaca
      181 gaaatcggaa aagaaaaact ttccggtcag gggtttgcca gctaccgtct caaattcaaa
      241 gtaaaaaaaa cagacacccc atttgctctg aaagcactgg atatggccac atcttactcc
      301 atctggttca acggtgaaaa aatctggtgg aatgggaaag tggcgaaaac caaggctgag
      361 tctgttccgg aattcaaacc caacacagtt tttctgtcca acgtaaaaga agaaaatgaa
      421 atcatcatag aagtctccaa ttatcatcat ttcaaaggcg ggttttggga aagcctcaaa
      481 atcggcagac tggaaaacct gatcctgttc agagaaaaaa gattcggatt tgatctgttt
      541 ttaactggaa gtcttctgat tatggggatc tatcatttcg gtctgttttc tctcagaaga
      601 aaggacaagt ccactctgct tttaggaatc ttctgcttca ttatgctctt ccggcttctg
      661 accaccggag aacggatctt tttcgtagtg tttccttttc tgggctggga tttctggctc
      721 cgaattgaat atttgagctt ctatctggcc acacctgtat ttgcctcatt tcttcattca
      781 ctgtatccgt acgaatttaa aagaagagcc acgcagatcg caattttagg ttcctctgtt
      841 ttctgtcttt ttgtaatttt cggcacagca aaatttttct cgagcactcc catccctttc
      901 cagatcttca ctgtcatttt cctgatttac ggattcatac agctgacacg ggcaagcatc
      961 agaaaaagag agggagggat tctcgctctc ataggattca ttatgcttct tctgaccaca
     1021 gtgaacgata ttttatatgg aaatttcatt ataaatacaa catatctttc tccttacggt
     1081 ttttttgctt tcatattcac tcaggctttc attctatctg tccgtttttc aaaagcattt
     1141 ctgaatgtgg aactgcttac agaggatctt cagaagacaa accatgcata cagcagattt
     1201 gttcctaaag aattcctgag ttatctgcag aagaacaata ttatggatgt caccccgggt
     1261 gatcaggtga aaaaggaaat gacgatccta ttctccgata tccgttcatt taccaatatc
     1321 tccgagaata tgactcctga agaaaacttt gatttcgtaa attcctatct ggcagtaatg
     1381 aatccgatca tcaaaaaaaa cagggggttc gtggataagt ttatcgggga tgcaatcatg
     1441 gctctttttc ctgactcacc ggaagatgca atcaacgcag gcatagaaat gcagaaagga
     1501 ctgaaggaat tcaacatcag acgtctgcag gaacataaag aactgatcca ggtaggaatc
     1561 ggaatccata caggcagcct gatgctcgga acagtcggtg attcggacag aatggaaggc
     1621 acagttatca gcgatgcagt gaacctggca tcaaggattg aaggaatcac aaaggtgtac
     1681 ggagcaaaca tcattatcag cgagcagaca ctgacagcac tgccggacac ttctcagttc
     1741 gctctgcgct ttctgggaaa agtggaagtc aaaggcaaga aggacccggc aactgtattc
     1801 gagatatatg aagcagacga tgcagacgtc cgcgaaaaga aaattgaatc acagattatg
     1861 tttgaaaaag gaattcatga ctttctgaca ggcaatcccc acaatgcaaa acttctgttt
     1921 gaagaaattc tggaaagaaa tccaaaggat ctgcctgcat acaaatatct gcagaaatgc
     1981 agtctgattg attcggtaca gtctgcaaat gctgattact gagattttct tttatagaca
     2041 gccgcacagc tgaacttgcc cgattttttc tgcgtcaaaa caagctctgt gcctgcaagt
     2101 ttctgcagtg attcagcgga aacatcacag ttttttcccc cgatcagaat ttgactgaat
     2161 gcattctttt tcttttgaaa atcttctgca cgaaaagaga gtttcactct gtttttaaag
     2221 gtcagaattc tgatgacaag ttccgtatcc ggcttgttgt agtgccaatc ctctacagta
     2281 ctcagaaaaa ccgtattttc cctgaccaga tctgcagcca atgtcttcag ctctctggga
     2341 gtctcataga tattctgctt tcctgaaaaa cataaattta cattctgaag acggagagga
     2401 attttcgtca tctgatcgtg cataaagaaa agagcagtgc ccagacagac ggtctgaaaa
     2461 agaagaacag aatatttcct tgcattgctt tgaagaatat aggaaaagaa cttcagttct
     2521 tcagttcttg ttattagagc agatatgtat tttataatta taaaacatgc gtccctgaaa
     2581 ccgtaaagag caatgaacgg ccaaagagga agaaggtgaa ggatatgcct tttctgatga
     2641 tttggagtca gaacactcag tccaagaatt ccggcaaaaa ggaaaaagaa aaagtattga
     2701 aaccgactga catgcctgaa cccttttcga aatgccagaa ataaaaacaa ggtcagtgca
     2761 aaaaggaatg tccctgaaat gattatggaa tacggtctgt cccaaaagga aaaaaataca
     2821 gtcttgaaaa agtacagata ataatccaga ttcttctcta ctgcctctcc tactccgtaa
     2881 ccttccatct gcacatggga tatggtgcct gcagaacttc cgagtctgtc cggatgagaa
     2941 atcacccata agacagaagg aagaaaaaga tatttaaaaa tatttattat tttttcatat
     3001 cccagattga aaagagaagc tttttccctg ataatataca gaacaaagtc agcagtgcct
     3061 gaaatcagaa gtacataaaa aatattcttt gagagtctgg atgaaatatg tcctttggat
     3121 aaaaggaaca gaatcagaag aacagcagag aatgagaata atccgctttt tctgtacaga
     3181 gagtaaagca gatatctgat gaagaagaat gcagaatatg atgtctcact gaaaaagaga
     3241 aacaggaaaa gacagaacag aaacatatac ccgtaaggat attttgtctg cagcagaaga
     3301 aaaatactga ttcccgcctt cagcagttct gaatgcgacg ggcttttcct tctgacacat
     3361 atataggaaa aatgaacact cagaagaaaa aacagtgcac cctgaatttc aagcatcggt
     3421 gaaaaaatat agatgataaa atctgatgca gacagcagtg aaaagaaaag aagaaccgaa
     3481 aagagaagac ctgcaaaatg aaaatccttc cagagaatca ggagcacaca aaagatcatg
     3541 agtccaaact gtacaacagt gatgagactg tcataatcag tattcagtcc ccagatgccc
     3601 aaaacggcag cctgaatcag attgcgcaga accggccaag tgggggtgtc ca
//
LOCUS       DAOQGJ010000138         3646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14384_length_3646_cov_3.462545, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000138 DAOQGJ010000000
VERSION     DAOQGJ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14384_length_3646_cov_3.462545"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            583..978
                     /locus_tag="PK683_03210"
     CDS             583..978
                     /locus_tag="PK683_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07484.1"
                     /translation="MAVNLKFKDETMTGNVLRTWTVPVVYERAAVKEIISTRVQREVE
                     KYNQSAEDYFNGLVQPSSAEVSLNGYRQKKKQYIDPAEQTETALKGFSANAFIVLIND
                     RQAESLEEIVEVNENTEISFIKLTPLVGG"
     gene            980..3562
                     /locus_tag="PK683_03215"
     CDS             980..3562
                     /locus_tag="PK683_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HNH07485.1"
                     /translation="MNIEILKEMIENHLPKESDSGLKEYDSLILDIFTDIYIPKSYRV
                     KKISESPNYKKFEKEKDEFWKGFAVRVQDFLIAMWNIPESHERYNILNEDSFYVRIAL
                     SEIYKGAVRTMRNFSEEEIVRLSEKNVEARICNEEYHLGIKQMQNFLKKNSATDILKN
                     HIKYMLGHDEIYIKRHYQEKNAEKIRLAVQEMYNKITGTASEVPAVLWSDEDSFGKGI
                     NEDVAGFAEDEKKTLYYKIFHAFKKASGGKPGAKYIKETEELIQKIGEKHFYEHSLRW
                     MKLFLDYEVKKETITSQGYNGREYHYDRYYNISGVNADSLKAMIWASVSVLDETMIRM
                     LGQAAEKSYKKIPGKGPANAAVGNACFYTLAASQNMEAISILSRLKLRLKQSSVIKLI
                     DSYISEFADEKGVSPHDIEDMAVQEYGLVKGCADHIFGDYKVHIEIRRVGDVEVQWTD
                     SKGRTLKSVPASAKTEFKSDLKKMNEDLKEIQKTLTVQRDRLDRSYIYERKWSWKDFE
                     KRFTGHGLMHCIAKNLIWNLHSKGKTEVLLFDGEDWINEDGHSEKISDIENVSLWHPA
                     GQGLETVMKWRELILSKELVQPFKQAYREIYLLTDAEVRTAVYSNRMAAHILKQHQMN
                     TLMKGRTWTYSLLGAYDDGRDGETARLQIPAHGLTAQFWIQELFIQDAFNDAGIWDFV
                     GTDQVRFVNAAGQAQDLTRIPVLIFSEVMRDVDLFVGVASVGNDPEWRDRGDLSNAQR
                     TYWEGYSFGDLNEMAKMRKSILEKILPRLKIASVAEIKDKFLVVKGKKRTYKIHIGST
                     NILMEPNDQYLCIVPDRKKDINDKIFLSFEGDNGLSLLISKAFLLAEDDKIKDETIIR
                     QIEK"
BASE COUNT         1210 a          630 c          879 g          927 t
ORIGIN      
        1 tatcccggct gggaggcggc tgcagtttta caggaaatca gagaagaatt ttcccatttt
       61 ttccaaagaa agattgatat cgccaataag aatgcactaa gaggaatttc tggagaaaac
      121 attctatcac aggtcctcta tgtctaaggg cagtgatgct gcaagaatcc ggtagattct
      181 agagtatatt tctgatattg aaaaaatcat tataagacac aaaacaatag aaacagcttt
      241 ggatgatctc gaggggcatc atgctgtttt aatgtgtctt ggacagattg gtgagaaaat
      301 gaataaactc gaatcctctg aatttcgagc tgttcttcct gtaagatacg cctaagcatt
      361 tcgaaatatt attatgcatg attacgaagg agtggatatc actatcacaa ttcagactat
      421 tcagaagagt ataccggagt taaaaaatca gattttagaa atactgcaga gtatttgaat
      481 gggatggact tgcatttgga tagaaacata ttttctgctt gaaaaatcac ccgcttggat
      541 aaactgactg caaatcttca gtcagtagag gtattataat atatggcagt caatttaaaa
      601 ttcaaagatg aaacaatgac aggaaacgtt ctgagaacct ggacagttcc ggttgtgtat
      661 gaaagagctg ctgtaaaaga aatcatcagc acaagggtgc agagagaagt ggaaaaatac
      721 aatcagtccg cagaggatta tttcaacggt cttgttcagc cgagcagtgc tgaggtttcc
      781 ctgaacggat acagacagaa aaaaaagcag tacattgatc ctgcggagca gaccgaaact
      841 gcgctgaaag gtttttctgc aaatgcgttt attgttctca tcaacgacag acaggcggag
      901 tctctcgaag agattgtgga agtcaatgag aataccgaga tcagtttcat caagctgaca
      961 ccgctcgtag gaggctgata tgaacattga aatactgaag gaaatgattg aaaatcatct
     1021 gccgaaagag tctgactccg ggctgaagga gtatgattct ctgatactgg atatttttac
     1081 tgatatctat attcctaaaa gttacagagt taaaaaaatt tcggaatcgc caaattataa
     1141 aaaatttgag aaggaaaagg atgaattctg gaagggtttt gcagtccggg ttcaggattt
     1201 tcttatagcg atgtggaata ttcctgaaag tcatgaaaga tacaatattc tgaatgaaga
     1261 cagtttttat gtccgtattg cgctgtcgga aatttataaa ggtgctgtcc gtacaatgag
     1321 gaacttttct gaagaggaaa ttgtccgtct cagtgaaaaa aacgtggagg cccgcatctg
     1381 taacgaagaa tatcatctgg gcataaagca gatgcagaat ttcctgaaaa agaactcagc
     1441 tacagatata ttaaaaaacc atattaaata tatgctgggt catgatgaga tttacattaa
     1501 aagacattac caggaaaaga atgcggaaaa aatcagactg gctgttcagg agatgtataa
     1561 taaaatcaca ggcactgcat ctgaagtgcc tgcagttctc tggtcggatg aggacagttt
     1621 cggaaaggga atcaatgagg atgtggccgg ttttgcggag gatgaaaaga aaacactgta
     1681 ttataaaata ttccatgcat tcaagaaggc aagcggaggc aagcccggtg caaaatatat
     1741 aaaggaaacc gaagaactga ttcagaaaat cggagaaaaa catttttatg aacattctct
     1801 gcgctggatg aaattgtttc tggactacga agtgaaaaag gaaacaatca catctcaggg
     1861 atataacggg agagaatatc attatgacag atactataat atcagcgggg tcaatgcaga
     1921 cagtctgaaa gcaatgatct gggcatctgt atctgttctg gatgagacaa tgatccgaat
     1981 gctgggacag gccgccgaaa agagctataa aaaaattccg ggaaagggac ctgcaaacgc
     2041 tgcggttgga aatgcctgtt tttatactct ggcagcttct caaaatatgg aagcgatcag
     2101 cattctttcc cgtctgaaac tcaggctgaa gcagagcagt gtcatcaagc tgattgattc
     2161 atatatctcc gaatttgcag atgagaaggg agtgagtcct cacgacatag aggatatggc
     2221 agttcaggaa tacggccttg tcaagggatg cgcagatcat atatttggtg attataaagt
     2281 tcatatagaa attcggagag tcggcgatgt ggaagttcaa tggactgatt caaaaggcag
     2341 aactctcaaa tctgtgccgg cttctgcaaa gactgaattc aaatctgatc tgaaaaagat
     2401 gaatgaagat ctcaaggaaa tacagaagac tctgacagtt cagagagaca ggctcgacag
     2461 atcctatatt tatgaaagaa aatggtcatg gaaggatttt gaaaagagat tcaccggaca
     2521 tgggctgatg cactgcattg caaaaaatct gatctggaat ctgcacagca aaggaaagac
     2581 agaagttctt ctttttgacg gggaggactg gatcaatgag gacggtcatt ccgaaaaaat
     2641 atcagatata gaaaatgtat ctctctggca tcctgcaggt cagggactgg aaactgttat
     2701 gaaatggaga gagctgattc tttcgaagga actcgttcag cccttcaagc aggcatacag
     2761 ggagatatac ttgctgacgg atgcagaagt aaggacagcc gtatattcca acaggatggc
     2821 tgcccatata ctgaaacagc atcagatgaa cactctgatg aaaggaagga catggacata
     2881 ctctcttcta ggggcctatg atgacgggcg tgacggcgag accgcaagac ttcaaatacc
     2941 tgcacatgga ctgactgctc agttctggat acaggagctc tttatccaag atgcctttaa
     3001 tgacgcagga atctgggatt ttgtgggcac agatcaggtt cggttcgtga atgccgccgg
     3061 tcaggctcag gatctgactc ggatacctgt gctgattttc tcagaagtga tgagagatgt
     3121 ggatctgttt gtgggagtgg caagtgtagg aaatgatcct gaatggagag accggggaga
     3181 tctcagcaat gcgcagagaa catactggga ggggtattct ttcggtgatc tgaatgagat
     3241 ggcgaagatg agaaagtcca ttctggaaaa aattcttccc cgtctgaaga ttgcatctgt
     3301 cgcagagata aaagacaagt ttctggtcgt caagggaaag aagagaacat acaagattca
     3361 tatcggcagc acgaatattc tgatggagcc gaatgatcag tatctctgca ttgttcctga
     3421 caggaagaag gatattaatg ataaaatatt cctctccttc gaaggagaca acggtctctc
     3481 tcttcttatc agcaaggcat tccttcttgc agaggacgat aagataaaag atgagacaat
     3541 catcagacag atagaaaaat aattcttgac cctttatccc tgtgaaatat tctttcctgc
     3601 atagattgca ggagagacat atgaaaaaat cgaaaatatt cagtct
//
LOCUS       DAOQGJ010000139         3634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14460_length_3634_cov_4.643196, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000139 DAOQGJ010000000
VERSION     DAOQGJ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14460_length_3634_cov_4.643196"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1772
                     /gene="glmS"
                     /locus_tag="PK683_03220"
     CDS             <1..1772
                     /gene="glmS"
                     /locus_tag="PK683_03220"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNH07486.1"
                     /translation="GREAVVAGHVAGVAVLDDGEIEIRKDQGKIRNLEKLLKEQPVEG
                     NIGIGHTRWATHGEPNAVNAHPHYNFHQSIAVVHNGIIENFDHLRKELQSKRFIFVSD
                     TDTETIPQLIDYNRRTFKEDFDLMKNTIAQLEGRFAFLVLMENRPDRLFFCKNGSPLI
                     YGRSENEGFLVSDSSAFIASVKEQYFIQDGEWGWITNKEIFVFDADGNPKQPEMVPVT
                     LDTSIYEKGSYDHYMQKEIFEQPLVVERILNERITDQGEIIFPEMRLNNDFQAKLFRV
                     VFTSAGTSLHAALIGRLYIEKFAKTMTEVEFSSEFRYRNPVLGGDTLVIGISQSGETA
                     DTLASVLAARAKFLKVLSFVNQINSSIARESTAVINLFAGPEIGVASTKAYVAELVSL
                     LLFSLHLARLKWVLTTEQVKEILDEVKKLPDQINAILAKSEEIRKIAEEMKEKRDFIF
                     LGRSFEHATALEGALKLKEISYAHASGYAGGEFKHGPIALVSREVPVICICPKNEVYT
                     KMVSNLEEVRARNGIIFSIYTEGDEQVPKLSNYSFSIPECSEYIAPILSVIPLQLLAY
                     YVAVAKGCEPDQPRNLAKAVTVE"
     gene            complement(1776..1928)
                     /locus_tag="PK683_03225"
     CDS             complement(1776..1928)
                     /locus_tag="PK683_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07487.1"
                     /translation="MSTLPSSPDFLLPFLAGGILLILGAWVFFIIRTYDDYRKENKGS
                     KKDSDP"
     gene            complement(1993..2667)
                     /locus_tag="PK683_03230"
     CDS             complement(1993..2667)
                     /locus_tag="PK683_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07488.1"
                     /translation="MGINERVSNWEIERRNRFLQETKGSTVIGNAVFSSPVFNERDRT
                     FIWKGTLNWWKWHPYFRGLRIGPIPAMLFIGLVLGLLFFLEASSVIPDKDGMPYSYYS
                     PVFLFWSAVSVGLPSFYYIYRVFFSETEISVINSILTVGRKKFDIRNSELNFKVWGAG
                     KTHEMPYARNYPGSVSLIFQREGKEIFTLYIGIDPYNRTICLEQIESAAKQIASAAGI
                     QFIQAV"
     gene            3057..3479
                     /locus_tag="PK683_03235"
     CDS             3057..3479
                     /locus_tag="PK683_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07489.1"
                     /translation="MKKIPETKQTALLRTDFSDDAAWKRVCKEATKDAGGVAAYLDFI
                     DDKTFLGLDPKKVKTVLPAGYNHLIIFFADQKTFADKKNTLLCVELGEDSKYRTFRVA
                     LKAMWEVENNLSIANMDFDDFADPDREALDANGVFNGF"
BASE COUNT         1101 a          757 c          785 g          991 t
ORIGIN      
        1 acggtcgtga ggcggtcgta gcggggcacg tcgccggagt tgctgtactg gatgacggtg
       61 agatagagat tcgaaaagat caggggaaaa tcagaaatct ggaaaagctt ctgaaagaac
      121 agcccgtgga aggaaatatc ggaatcggtc atacaagatg ggctactcat ggtgagccca
      181 atgcggtaaa tgcgcaccct cattataatt ttcatcaaag cattgcggtc gtgcataatg
      241 gaattattga aaactttgat catttgagaa aagagcttca gtccaaaagg ttcatttttg
      301 tttcagatac ggatactgag accatccccc agcttattga ctataatcgc agaactttta
      361 aggaagattt cgaccttatg aaaaatacga ttgctcagct ggaaggaagg tttgcatttc
      421 tcgtactgat ggaaaacaga cctgacagac ttttcttctg taaaaacgga tctcctctca
      481 tttacggcag aagcgagaat gaaggctttt tagtaagtga cagctctgcg ttcattgcat
      541 ctgtaaaaga acagtacttt attcaggatg gggaatgggg atggattacc aacaaagaaa
      601 tcttcgtttt cgatgctgat ggaaatccaa aacagcccga aatggttcct gtcactctgg
      661 atacaagcat atatgaaaag ggcagttacg atcattatat gcagaaagaa atttttgaac
      721 agcccctggt tgtagaaaga attctgaatg agagaattac tgaccaggga gagatcattt
      781 ttcctgaaat gagactgaac aacgattttc aggcaaaact tttcagagtt gtatttacca
      841 gtgcaggaac atctctccat gctgctttga taggcaggct ttacattgaa aaatttgcca
      901 agacgatgac tgaggtggaa ttctcttccg aattcagata cagaaatcct gttcttggag
      961 gagacactct ggtcatagga atctcgcaaa gcggggaaac agccgataca ctcgccagtg
     1021 tacttgctgc aagggcaaaa tttctcaaag ttctatcttt tgtaaatcag ataaactctt
     1081 ccattgccag ggaatcaaca gctgtcatta atctgttcgc aggtcctgaa atcggagtcg
     1141 catctacaaa agcttatgtt gctgaactag tcagtcttct cttattttct cttcacctgg
     1201 caagactaaa gtgggttctg acaactgaac aggtgaaaga aatactcgat gaggtaaaaa
     1261 aacttccaga ccagatcaat gcaattctcg caaaatcaga agaaatcagg aaaattgccg
     1321 aagagatgaa agagaaaaga gatttcattt ttctcggaag atcttttgaa catgcaactg
     1381 ctctggaagg agcattgaaa ctgaaagaga tttcctatgc tcatgcttca ggttatgcag
     1441 gcggtgaatt caaacatgga ccaatagctc tagtttcccg ggaagtacct gtcatctgca
     1501 tctgtcctaa aaatgaggtt tacaccaaaa tggtatccaa cctggaagaa gttcgggcaa
     1561 gaaacggaat cattttctcg atatacactg aaggagatga gcaggttccg aaactttcca
     1621 attattcctt ctcaataccg gaatgctctg aatatatagc tcctatatta tctgtcattc
     1681 cactgcagct ccttgcctac tatgtagcag ttgcaaaagg ctgtgaaccg gatcagccga
     1741 gaaacctggc taaagcggtc acagtagaat agatctcagg gatcagagtc cttctttgat
     1801 cccttgtttt ccttcctgta gtcatcgtaa gttctgatta taaaaaacac ccaggcaccc
     1861 aggatgagca gaattccgcc ggcaaggaac gggagaagga aatcaggaga tgagggcaat
     1921 gtgctcatta tactttcttt atacaagata caaactcagc ttgcaaacat tttttatttt
     1981 tgaactgctg tctcagaccg cctgaatgaa ctgaatacct gctgcagaag caatctgttt
     2041 cgccgcagat tcaatctgtt ccaggcagat cgttctgtta taaggatcaa ttccgatata
     2101 cagagtaaaa atctcttttc cttctctttg aaaaataaga gaaactgaac cgggataatt
     2161 tcgggcatag ggcatttcat gagtttttcc tgctccccag accttgaaat tcagctcaga
     2221 attccgaatg tcaaatttct ttctccccac agtaaggatg gagtttatta cactgatttc
     2281 tgtctcagaa aaaaatactc tatatatata atagaatgat ggcagaccga ctgatacagc
     2341 agaccaaaaa agaaacacag gagagtaata tgaatacggc attccgtctt tgtcaggaat
     2401 gacacttgaa gcttccagga aaaacagcag accgagcacc aggccgataa acagcattgc
     2461 aggaatagga cctatcctga gtcctctgaa ataaggatgc cacttccacc aattcagcgt
     2521 acccttccag ataaatgtcc gatccctttc attaaaaaca ggagaactga agactgcatt
     2581 gccgatcaca gtactgccct ttgtctcctg cagaaatctg ttccggcgtt caatctccca
     2641 gttggagact ctctcattta tgcccatcgg ataagagtac ggttctgccg ctgtcagtca
     2701 agttctttac gaattgccct cccggatttt acggttactt tactgtcaaa attataactc
     2761 acactgcgct tacgtctgtc gggcggtttg ggcaggaact cctgtcggcg gtttcgcaag
     2821 tgtggaaagt tgctgttttt actgattcat taaatttccc taatgtttta tgaaaattac
     2881 tgcgttcagg acagtatgtc ctatttacgt aagatgagtt ataaataaga aaagctgcga
     2941 tttggacaga attccctgct tgaaaacagt atttctgttc agatatagaa ctcaaacttc
     3001 cgatactgag tatattcact gatttaaaat agtaaattat tttaggagtt tgtccaatga
     3061 aaaaaatccc cgaaacaaaa cagaccgctc ttttgagaac tgatttttca gatgatgcgg
     3121 cctggaaaag ggtctgcaaa gaggcgacta aagatgcagg cggtgtggcg gcctatctgg
     3181 actttattga tgataagact ttcctgggac ttgatcccaa aaaagtaaag actgtactgc
     3241 ctgccggtta caatcatttg attatctttt ttgcagacca gaagacattt gctgataaaa
     3301 aaaatactct gctctgcgtt gaattaggtg aggacagtaa atacagaact ttccgagttg
     3361 cattgaaagc gatgtgggaa gtggaaaaca atctgtccat tgcaaatatg gacttcgacg
     3421 attttgctga tcccgatcgg gaggctcttg atgcaaatgg agtattcaac ggattttagg
     3481 tgcactgcat cgtcatcccc attgtactgc attctgcaca gacgaaaatg taaagatcct
     3541 gatcgccgca tccgaatcga accgccctga cacgagccgc atagtccatc gcttcctcgc
     3601 attcagggca gacagcccag ctgtcgtaac cctg
//
LOCUS       DAOQGJ010000140         3629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14493_length_3629_cov_3.498041, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000140 DAOQGJ010000000
VERSION     DAOQGJ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14493_length_3629_cov_3.498041"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..729)
                     /locus_tag="PK683_03240"
     CDS             complement(334..729)
                     /locus_tag="PK683_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07490.1"
                     /translation="MILFESTTAKTEYLPEKNMIVLTQQGKNVGESLKKCLDIGLDAL
                     IKNKAYKWLSDNRFTQSVRGKEDAEWVNNDWTPRALKAGWKKWALIQPKSALTSMAEQ
                     QFVDFFAENGIEVKIFDTPEEGFAWLDTV"
     gene            1080..1490
                     /locus_tag="PK683_03245"
     CDS             1080..1490
                     /locus_tag="PK683_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07491.1"
                     /translation="MRLIFEESGVATIHRVDADRMIILKWLKFNAAEEFKTVLNNVRD
                     AFAETKYARVLVDTSETKGMISAENQKWMEELHFPKLLEQNICKYVATVVPGTVLGQM
                     TTEQWQDNLTKIADMELINVKSSDEGISWLKGKK"
     gene            complement(1530..2117)
                     /locus_tag="PK683_03250"
     CDS             complement(1530..2117)
                     /locus_tag="PK683_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07492.1"
                     /translation="MKQFDMGNPEIEMIGSAALSVVNGIKTSFSQKIIQGILKDNGLE
                     NVEAEKWYQTQKWLNAFRTISEQIGANTVFLTGMAIPENALLPPEISTIEAALSAINI
                     AYHMNHRLNGEPLFDPSSGRINDIIGNYKCNILGDRKVQMICDNPYPCDFDRGLIEGF
                     ARKFKSPGSSGVSAVHSSRECRKNGDDFCEYTVTW"
     gene            complement(2624..>3629)
                     /locus_tag="PK683_03255"
     CDS             complement(2624..>3629)
                     /locus_tag="PK683_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07493.1"
                     /translation="NTHKYEKRPKTVSSDISFSSQNLDVKIDRKERQKGLLKYPGYIL
                     SFKGEYIIKNTRNKSTNLYFLFQLPQNAGNITDIQVLLNDKPYKGDTNLADGISWSEN
                     MEPDEENKITVSYKAQGTETFSYLLGSTHKEIKQLKAVLNTDFRHYNIPDHAMVPSSE
                     ENESGKTKILWNSENLITGQNISLKFEIEGNYGKIASKLFFYSPLAIFLFLALLLLLT
                     VSKGTVLHPMHYLFIIISFFIFYLLGSYLISYMHIFFGILASLAVSTGIMIYYAFLMK
                     KGKILIQGIALSAFVFQWFFSIAFFFPEHTGFLITAATIIAFVFLLKTTAETDWEDKF
                     "
BASE COUNT         1038 a          702 c          735 g         1154 t
ORIGIN      
        1 ctgccgattg taatgattat aatcctgaaa cctgtaattt tttatgaagc attggagaaa
       61 tccagcagtc tgacgcttgc gataaatcag taaatgcagc acagccaggc ttccggcagc
      121 agccgagttt gcgaatcatt tgcccgcata cagactttcc tgccaaagaa gctccagctg
      181 catacggagt cgtaagataa gtaaaattga taataattat ttttactgta taatttcaga
      241 gagttttgtc cggatacaat tccgccgaca ggattttgcg gcggctttgc atccgatctg
      301 tcttcttcag cagacttatc tgctgaattc tcatcagact gtatccagcc aggcgaatcc
      361 ttcttccggt gtatcaaaga ttttgacttc aatcccgttt tctgcaaaaa aatcaacgaa
      421 ctgctgttca gccatggaag tgagtgctga tttcggctga atcaatgccc attttttcca
      481 gcctgccttg agtgctcttg gagtccagtc attgtttacc cattctgcat cttccttgcc
      541 tctcactgac tgagtgaatc tgttgtcgga gagccatttg tacgccttgt tttttatcag
      601 tgcatccaga cctatatcca ggcatttttt cagactttct ccgacgtttt ttccctgctg
      661 tgtcagcact atcatatttt tctcaggcag atattctgtt ttagctgtgg ttgattcgaa
      721 aagtatcata tattttcctc tgaatcgaat ccgtttcctg ccgagtaaaa aatcaacttt
      781 ttttgcagaa atgcagattt tcttttcata tatcagttta tggatttttt ttaatgatta
      841 aaacatacgg ttctttcata aacgttcagt agagaagatc tgaaacattt gcaaaactgt
      901 gttttttttc gttgaatgtc agtctgtatt ctgcagaaaa tcattatctc ttcgcattta
      961 tgtaagaata atttaatata ttctataata gtatataaga gattttcttc tgtcattcct
     1021 gataaaacta attgaaaaaa tcagaccctg ctgaatgact gcataagagg agaaagataa
     1081 tgagacttat tttcgaagag agcggagtag cgacaatcca tagagttgat gcagaccgta
     1141 tgataattct gaaatggctg aagttcaatg cggctgagga attcaagact gtactgaaca
     1201 atgtcaggga tgcttttgct gagaccaagt atgcacgtgt tctggtcgat acatctgaaa
     1261 ctaagggaat gatttccgca gaaaatcaga aatggatgga ggaattgcat ttccctaaac
     1321 ttctggaaca gaatatatgc aaatatgttg caacagttgt acctggaact gtattaggtc
     1381 aaatgacaac ggaacagtgg caggacaatc tcactaaaat tgcagatatg gagctgatta
     1441 atgtcaaatc ttctgacgaa ggaatcagct ggctgaaagg aaaaaaataa tacgctaggc
     1501 tgtctgtcaa aagagaggca gccgacggac taccaggtga ctgtgtactc gcagaaatca
     1561 tctccgttct ttctgcattc tcggctgctg tggactgcgc ttacgcctga agaccctgga
     1621 gatttgaatt ttcttgcaaa accttcaatc agtcctctgt cgaaatcaca cggataggga
     1681 ttatcacata tcatctgcac ttttctgtct cccaaaatat tgcatttata attaccgatg
     1741 atatcattta tccttcctga agacgggtca aagagaggtt caccgttcag ccggtgattc
     1801 atatggtatg cgatgttgat tgccgagagg gctgcttcta ttgtactgat ttccggagga
     1861 aggagggcat tttccggaat tgccattcct gtcagaaaca ctgtattggc accgatttgt
     1921 tccgagattg tcctgaaagc attcagccat ttctgggttt ggtaccattt ctcagcttct
     1981 acgttttcca ggccgttgtc tttcagtatt ccctgaatga ttttctgaga aaatgatgtc
     2041 ttgattccgt ttactacgga cagtgcagca cttccgatca tttctatttc aggattgccc
     2101 atatcaaact gcttcattac cgcctccagt tgaaaatccg gatctcagat caatttatcc
     2161 gactgaaaag caataatcat acagatttat actgtgtcaa ttgaaataat aaaagtgtta
     2221 gaatttagta aataaaggaa tataataatg aattttagaa tcatctgaaa agatgaattt
     2281 tcgaagatga aatagagttc attgtcggat aaatgttttc aggggaatct ccgaacattt
     2341 aaattgcaga aaatcgagaa tgtatgcttg gccattttac tgcagatttt acattccagt
     2401 gattctgcct gctgttgact tttatgatca actgcagact taaaaacaat agctgtcctt
     2461 gataaatgca gattatacga attcctgtat ttttgctgaa ggcgaattat tcccttccga
     2521 ttgcggaaag atacagcctt cagaaaattt cctttcagat tcagaaagaa gagtctgttt
     2581 actcagccga aaggaaatat gttctgcggt attctctgtc cttctaaaac ttatcctccc
     2641 agtcagtttc tgcagttgtt ttcagtaaaa atacaaatgc aatgatcgtg gctgctgtga
     2701 tcagaaatcc tgtatgttca gggaagaaga aagcaatcga aaagaaccac tgaaaaacaa
     2761 aggcagaaag tgcaatgccc tgaatgagga tctttccttt tttcattaag aatgcataat
     2821 aaatcatgat tcctgtggaa actgccagtg aagccagtat cccgaagaag atatgcatat
     2881 aactgatcag ataggaaccg agcaggtaaa atataaagaa acttattata atgaaaagat
     2941 aatgcatcgg gtgaagcact gttcctttgg aaacagtcag cagaagcagg agggcaagaa
     3001 acaggaaaat ggcaagcgga gagtagaaga aaagcttgga tgcaatcttg ccatagttcc
     3061 cttcaatttc gaatttcaga gagatgttct gtcctgtaat cagattttca ctgttccata
     3121 aaatttttgt cttccctgat tcattttcct ctgaagaagg caccattgca tgatccggaa
     3181 tattgtaatg tctgaagtca gtgttcagaa cagccttcag ctgtttgatt tccttgtgag
     3241 tcgagccgag aaggtaggag aatgtttctg ttccctgggc cttgtagctt acagtgattt
     3301 tattttcttc atccggctcc atattctcgc tccaggagat tccgtctgcc aggttcgtat
     3361 ctcctttgta aggtttgtca ttcagaagaa cctgaatgtc ggtaatgttt cctgcgtttt
     3421 gaggaagctg gaataaaaaa tacaaatttg tacttttatt tcttgtattt ttaatgatat
     3481 attctccttt gaagctgagt atgtatcccg gatacttcag gaggcctttc tgtctttcct
     3541 tcctgtcaat tttgacatcc agattctgag aactgaagga gatgtcagag ctcacagtct
     3601 tcggtctttt ttcatatttg tgagtgttc
//
LOCUS       DAOQGJ010000141         3628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14503_length_3628_cov_3.369158, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000141 DAOQGJ010000000
VERSION     DAOQGJ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14503_length_3628_cov_3.369158"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK683_03260"
     CDS             <1..288
                     /locus_tag="PK683_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07494.1"
                     /translation="LSFFIGLILGPFQPFFVGAVQGWILGRGSFDYILEKNTENLKER
                     DRKASEFRMEIFGLGIAQFIFLFIPFIGVILGPIAAVIGSFLIYYRDRIKD"
     gene            complement(297..1367)
                     /locus_tag="PK683_03265"
     CDS             complement(297..1367)
                     /locus_tag="PK683_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH07495.1"
                     /translation="MNMTAEIIDVHHHFVPDFYADALNKAGYRKVAGAELPDWTPERS
                     LKVMDMNGIRTAVTSISSPGVFFGNAENASLLARKCNEYMAELKIKFPERFGSFAVLP
                     MPLPDRSVLEAVYALDQLKCEGIVLLGSTEGKFLGHPDFDALMEELNRRNAAVFVHPN
                     LHRTSELIDLSIPGFIVEFLCDTVRAAVNLIYSGTMEKFPNIRWVLSHAGGFLPYIAW
                     RVSLGNLFPDVNEKAPAGILNYIKRFYYDTALSPSPYVVSALKELVEPDHILYGSDFP
                     FAPESLVFYQREEFEALKQIDESERRKICRINSLKLFPALASKEEKTGKVLPAEKTSI
                     SQKLKFRMNRTAVSVLSKLMKR"
     gene            complement(1409..2290)
                     /locus_tag="PK683_03270"
     CDS             complement(1409..2290)
                     /locus_tag="PK683_03270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNH07496.1"
                     /translation="MNSKRITVRRPNYIFTSDIPEHWFAESALFSHIGNSLFLSFPQG
                     ERFFIHSVQAFAKQVQDTALREEIKAFVAQEVQHTKEHENHFEILRAQGYDVEGILNF
                     YRIYATEGIEVIAEKLFGKRMHLAMTAASEHFTALFSELAFEWNLPEYMHPQIADLTS
                     WHGAEEMEHRHIAFNVMQETGISYPERMLGLFFTVILLSGLTVSGTAKLLLNDKALDL
                     RKLGEDFLFLLKGFFPVTHTVTDHEGTQKKRTEAFGFLGQLIIGIVDYIRPNFHPDDR
                     NSRHLAAAGLKQFEKSA"
     gene            2423..3028
                     /locus_tag="PK683_03275"
     CDS             2423..3028
                     /locus_tag="PK683_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH07497.1"
                     /translation="MKKKNSTKQRLLAAAMEMYVRREREEINISDLAKTAGVARGTVY
                     NNLGSIDDLLERVTDYLTEEMNLNLEKQFQSVSDPAKRVSYGIRLYVYRAKNEPLWGE
                     FLCRFGMESASLQALITGPMGKDIQRGLEEKRFQFREEQFKTVIALIGSSVLTAFYLV
                     KEGISTWTEAGADCAEFALRSLGIDQAEAAKIGSEKIPSIR"
     gene            complement(3243..>3628)
                     /locus_tag="PK683_03280"
     CDS             complement(3243..>3628)
                     /locus_tag="PK683_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07498.1"
                     /translation="VNDEMESLIKNFEKEKQDAWKKKVYWNQKYISADTFWADWPLTV
                     KEGILTCYPRYAGGRGVNKIGIRAVTFRDREGNEYYLEFDESCWGCTRNIKNVLKPYL
                     EDGSIQLSLKKLILEGRELCGRFED"
BASE COUNT         1063 a          784 c          731 g         1050 t
ORIGIN      
        1 ctttcatttt tcatcggtct gatcctgggc ccttttcagc ctttctttgt aggagctgtt
       61 cagggctgga ttctgggaag aggaagtttc gactatattc tggaaaagaa tactgaaaat
      121 ctgaaggaaa gagacagaaa ggcctcagaa ttcagaatgg aaattttcgg actcgggatt
      181 gcacagttta tctttctctt cattcctttc ataggcgtaa ttctaggacc gatagcagct
      241 gtcatcggct catttctgat ttactacagg gacagaatca aggattgaaa tattcattat
      301 cttttcatta acttggaaag aacagagaca gcagtccgat tcattcggaa tttcagtttc
      361 tgtgatattg aagttttttc cgcaggcaga acctttcctg tcttttcctc ttttgatgcc
      421 aaggcaggaa aaagtttcag agagtttatc ctgcagattt tccttctttc tgattcatca
      481 atctgcttca gggcttcaaa ttcttctctc tgataaaaaa caagagattc cggagcaaac
      541 gggaaatcgc tcccatagag aatatgatcc ggttcgacaa gttccttaag tgcacttacg
      601 acatacggag aaggagaaag agcagtatca taatagaatc tttttatata attcaatatt
      661 cctgcaggag ccttttcatt tacatcagga aacagattgc cgagagatac cctccatgca
      721 atgtaaggca gaaatccgcc ggcatgagaa agcacccacc tgatgttcgg aaacttttcc
      781 atcgtgcctg aatagatcag attcactgca gcccggactg tatcgcagag aaattcgaca
      841 ataaatccag gaatggaaag gtcaatcagt tcacttgttc tgtgcaggtt cggatgaaca
      901 aatacagcgg catttcttcg gttcagctct tccatcaggg catcaaaatc aggatgaccg
      961 agaaacttcc cttcagtgct gccaagcaga acaattcctt cacatttcag ctgatcaagt
     1021 gcatagactg cttcaaggac tgatctgtcc ggcagaggca tcggaagcac tgcaaatgag
     1081 ccgaatcttt cagggaactt aattttcagc tctgccatat attcattgca ttttcttgca
     1141 agcaaagatg cattttccgc atttccaaaa aatactccgg gagaagaaat ggaagtgacc
     1201 gctgtcctga ttccgttcat atccataacc ttaagcgatc tttccggtgt ccagtccgga
     1261 agttcagcac cggcaacctt gcgatagccg gctttattca gagcatcagc gtaaaaatcg
     1321 ggcacaaaat gatggtgaac gtctataatt tcggctgtca tattcatata attctcctaa
     1381 agtatctgcg catatcataa gtcttttttc aggcagattt ttcaaactgc ttcaggcctg
     1441 ccgcagccag atgcctgctg tttctgtcgt caggatgaaa atttggtcta atataatcaa
     1501 caatcccaat tatcagttga cctaagaatc cgaaagcttc tgtccttttt ttctgtgtcc
     1561 cttcatgatc tgttacagta tgagtgacag ggaaaaagcc tttcaacaga aaaaggaagt
     1621 cctctcctaa tttccttaaa tccagtgcct tgtcgttcag aagcaatttt gcagttcctg
     1681 acacggtcag accagaaaga agaatgacag taaaaaacag tcccagcatt cgttcaggat
     1741 aactgatgcc tgtttcctgc attacattga aagcgatatg cctgtgctcc atttcctccg
     1801 ctccatgcca tgaagtcaga tcagcaatct gaggatgcat atattcagga agattccatt
     1861 cgaatgccag ttcagaaaaa agtgcagtga aatgttctga ggctgctgtc atagccagat
     1921 gcattctttt tccaaataac ttctctgcta tgacttcgat cccctctgtt gcataaattc
     1981 tgtaaaaatt caaaatgcct tcgacatcat atccttgagc cctcagaatc tcaaaatgat
     2041 tctcatgttc cttcgtatgc tggacttcct gtgcgacaaa tgcctttatt tcttcacgca
     2101 gggcagtatc ctgtacctgt tttgcaaaag cctgaacgct gtgaatgaaa aacctctctc
     2161 cctgaggaaa agacaaaaaa agcgaattcc caatatgact gaaaagagca ctttcagcaa
     2221 accaatgttc tggtatatca gatgtgaata tgtaatttgg gcgacggact gtaattcttt
     2281 ttgagttcat ataagtctcc ttgcttgttg ctgaagccat cgtaaatctt tgtttaaatt
     2341 tgaacagtat tatttatttt tgaacaatag tgtataaaaa taaattttaa ctgaacagtg
     2401 accgaaaggt gaatgtacga gtatgaaaaa gaaaaattcc acaaaacaaa gacttctggc
     2461 tgcagcgatg gaaatgtatg tcagaagaga gcgggaagaa atcaatatca gtgatcttgc
     2521 aaagactgcc ggcgtagcaa ggggaaccgt atacaataat ctcggttcaa ttgatgacct
     2581 gctggaaaga gtcacagact atctgacaga agaaatgaac ctgaatctgg aaaaacagtt
     2641 tcagtcggta agtgatcccg ctaaaagagt ctcttacggc atcagacttt atgtttatcg
     2701 ggccaagaat gaacctctct ggggagagtt tttatgcaga tttggaatgg aatccgcatc
     2761 ccttcaggct cttatcacag gaccaatggg aaaagatatt cagcgcggac tcgaagaaaa
     2821 aagatttcag tttcgggaag aacagtttaa aacagtgatt gcactgatcg gaagttctgt
     2881 tctcactgcc ttttatctgg taaaggaagg tatcagcacc tggactgaag caggagcgga
     2941 ctgtgccgaa tttgccctca gatcattggg catcgatcag gcagaagcag caaagatagg
     3001 atctgaaaaa attccttcaa ttcggtaatt gcagcaggac agtattgacg aacccctgct
     3061 taagcagaga ttatcactgt attcagtaaa accacagagg cagttatgga aacggaactt
     3121 ggacgatctc gggggtattt atatgtacaa tctgaacatt taatctgaac gcaagaaaaa
     3181 ctgcagacag aattctttga atgtctgaat cgaaaaaact cgagcagtga cttgaaattt
     3241 tttcaatcct caaaccttcc gcatagttct ctgccttcca atatcagttt tttcaggctc
     3301 aattgaattg atccgtcttc cagatatggc tttaatacat ttttaatatt tcgcgtacag
     3361 ccccagcagc tttcgtcaaa ttccagatag tattcattgc cttctctgtc ccgaaaggtg
     3421 actgctctga ttccgatttt atttacacct ctgccgcctg catatcgagg atagcaggtc
     3481 aggattcctt cctttactgt aagcggccaa tctgcccaga atgtatccgc actgatgtat
     3541 ttctgattcc aatatacctt cttcttccag gcatcctgct tttccttctc gaaattctta
     3601 ataagtgatt ccatctcatc attaaccg
//
LOCUS       DAOQGJ010000142         3618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14568_length_3618_cov_2.911591, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000142 DAOQGJ010000000
VERSION     DAOQGJ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14568_length_3618_cov_2.911591"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PK683_03285"
     CDS             <1..624
                     /locus_tag="PK683_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH07499.1"
                     /translation="AYLKTLIHLCEEKGVIFLKDRKFSHNDGISCLTDSRSAKEQVDP
                     GIIINAAGLYSDMIAQEFGLSEYSIRPNKGEYFRFRKPLPYKKLIYPVPSKKSTALGV
                     HYTFNMGDEAYAGPNSEWAENKEDYKITADRKIYYQSVCNILDCYSEEDLSPGYAGLR
                     PRLFRNGGPLQDFVIIKHPKNVIHLLGIESPGLTSSPSIAEEVLNMI"
     gene            complement(816..1286)
                     /locus_tag="PK683_03290"
     CDS             complement(816..1286)
                     /locus_tag="PK683_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07500.1"
                     /translation="MLIRNYDNIVKYLYVYDFSANLKYYSFSASGALNPVTTDTVRSG
                     SVKSAVSERSGKFLFFSADGFILPGTVNSIDGTLNSSPFTSYTNLQGTASDSAGLLLF
                     ASASNAGSYELFSCSISQGNGIMPYAASSSDNSIYKIHYTLTVPCSLLFPLLQV"
     gene            1784..2575
                     /locus_tag="PK683_03295"
     CDS             1784..2575
                     /locus_tag="PK683_03295"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH07501.1"
                     /translation="MIEIISLIAFFAGTSILTAGYFYQKKMIFYPSKTDRNYRYGFGR
                     PSEEIFFKRGSDELHSLHFPHPESEGFIVYFHGNAGDLAGWGNIEKDFRKFPLDLYIL
                     DYRGYGKSTGNIGSEKELISDCEAFYQEISSRHPYARKKIVYGRSIGTGPAVYTASRN
                     PATHLILETPYSSLKSLINKIFPIVPSFLISFTLESDVWIQNVNAEILILHGTDDSVI
                     PFSEGKQLSDSIRKKHDFIIIKKGDHNDLSMYKEYHTALEEFLKK"
     gene            complement(2765..>3618)
                     /locus_tag="PK683_03300"
     CDS             complement(2765..>3618)
                     /locus_tag="PK683_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HNH07502.1"
                     /translation="MYLHFHNLSGISWTRHSENILSKERPDIVIYALGVNERNWLKDS
                     IDLCDREGIELKIVPGFTEFIAARGRVEGMDGIPVISIRDIPVRYGYNLFLKRLFDIV
                     FSLTFIIVFSPVYLLIAFFIKITSKGPVFIFQERIGLDHRKFRMIKFRSMIVQEKSAS
                     DTVWTTKDDPRVTSIGKIIRKTSLDEIPQFFNVLRGNMSVVGPRPERPHFVDQFRTEY
                     SQYMRRHGVKGGITGWAQVQGLRGDTSIEERVAADIYYIENWSFFFDIKIILMTPLTG
                     FINKNAY"
BASE COUNT         1129 a          772 c          756 g          961 t
ORIGIN      
        1 gcctatctga aaacactgat tcatctctgc gaagagaaag gagtgatttt tctgaaagac
       61 cgcaaatttt ctcataatga cggaatatcc tgtctcacgg actcgcgttc agcaaaggaa
      121 caagtggatc ccggcatcat cataaacgcg gcaggtctat attctgatat gatcgcacag
      181 gaattcggtc tttcagaata ttcgatacgg ccgaacaagg gggagtattt ccggttcaga
      241 aaaccccttc cctacaaaaa acttatttac cctgttcctt caaagaaaag tacagcatta
      301 ggagtgcatt atactttcaa tatgggagac gaggcatacg caggaccgaa ttcagaatgg
      361 gcggaaaaca aggaagatta taaaattact gctgatcgga aaatatatta tcagtctgtc
      421 tgcaatatcc tcgactgcta ctctgaggaa gacctgtcac ccggatatgc aggacttcgc
      481 ccgaggcttt tcaggaatgg agggcctttg caggattttg taattataaa acatccaaaa
      541 aatgtcatac atcttctcgg aattgaaagt ccaggactca cttcctcccc gtccattgca
      601 gaagaagtgc tcaatatgat ctgatgcttc ggtgcagaat cactctgtga gagagactgc
      661 agcaataccc ccggaccggc agtacctccg gcaaaggaac tctgaaggct taaggaaccg
      721 tctgaggaac tgatcaggca taaccctggt attggcgctg tcggcagcag acagaaatct
      781 gcccgcagga tctgtcagca cggcattcgg catattcaga cctgaagcag cggaaacagg
      841 agactgcagg gtaccgtcag agtataatga attttatata tggagttgtc ggaagatgat
      901 gcagcataag gcataatgcc atttccctga ctgatgctgc aggaaaaaag ctcataactt
      961 cctgcatttg atgccgatgc aaaaagaagc aggccggcag aatcagaagc tgttccctga
     1021 agatttgtat aagatgtaaa aggcgaggaa ttaagtgttc catcaatact gtttacagtt
     1081 ccaggcagaa taaatccgtc ggcactgaag aatagaaatt ttcctgatct ttcagaaact
     1141 gcggatttta cagatccgga acggacagta tccgttgtga ccggattaag agcacccgag
     1201 gcggaaaacg aataatattt cagatttgca gagaaatcgt agacatacag atatttgact
     1261 atattatcat aatttcttat cagcacggat gcgggcagag aattttctcc tttggaattg
     1321 gaagagcagg caagcaggtg agtgactcct gacgggactg cagtattctc atttaatgtg
     1381 aaggaaatat cagaaccagt tttcgaaact gatttcagct aaaccgagcc gtattttgca
     1441 gttcccgaaa tccgaaatat atattataag catctgtatc agattcgtct gcagccctga
     1501 aaacagtcac tgcgcctccg atttttccat agttcagaag attcgcggtg tgtaccgttc
     1561 agcacagggt gaactgtgat aaagaaaatt tcggccttga attttcgaac aggtcagttt
     1621 gcaccctgac tgaaaagtca tcagtttcag gcattcagat aaggatctgc acagccttct
     1681 ctgcatcact gaaataagaa aaaaactgac tgttttcaga tatataatca gtcattcgag
     1741 aaaaagtttt gactgcagac tgcagtaaaa tcatgcgtaa tgaatgatcg aaataatttc
     1801 tctgattgca ttttttgcag gaacatccat actgactgcc gggtactttt accagaagaa
     1861 aatgatattt tatccctcaa agaccgacag aaattacagg tacggcttcg gaagaccttc
     1921 tgaagaaata tttttcaaaa gaggcagcga tgagcttcac tcactgcatt ttcctcatcc
     1981 ggaatcagaa ggattcatag tatatttcca tggaaatgca ggggatctgg caggctgggg
     2041 aaatattgag aaggatttca gaaaatttcc gctggatctt tacattcttg attacagagg
     2101 ttatggaaaa agcacaggaa acatcggttc agaaaaggaa cttatttcag actgtgaggc
     2161 attttatcag gagatatctt caagacatcc ctatgcacgg aaaaaaattg tatacggacg
     2221 gtccatcggc acaggccctg cagtatacac agcttccaga aatcctgcaa cccatctgat
     2281 tcttgaaacc ccttacagca gcctgaaatc actgattaat aaaatttttc cgatcgttcc
     2341 ttcttttctg ataagtttca ctctggaatc agatgtgtgg atacagaatg taaatgcaga
     2401 gatactgatt ctgcacggaa cagatgactc agtaatccct ttttctgaag gaaaacagct
     2461 ctccgactca atcagaaaaa aacatgattt tataattata aaaaaaggag atcataatga
     2521 tctttcaatg tataaagaat atcacactgc ccttgaagaa tttctgaaaa aatgacaggc
     2581 cctatgaggt gcctgccctt tttcagccag aaaaaggctt gacatttatt cagggctagt
     2641 tctcaacagg aaaaaataat taaaagcaga cggttcatca gtttcagaat cagcgggaag
     2701 taaagcccgg aacttcactg agacatatca gctcatctga aacaggaagt gagcaaagtg
     2761 agattcagta tgcattttta ttgatgaatc ccgtcaacgg ggtcatcagt ataatcttga
     2821 tatcaaagaa aaatgaccag ttttctatat aataaatgtc cgcagcgacc ctttcttcaa
     2881 tcgatgtatc acctcggagt ccctgcacct gcgcccatcc ggtgattccg cccttcactc
     2941 catgtcttcg catatactga gaatactcag ttctgaactg atctacaaaa tgcggccttt
     3001 ccggtctcgg gccgacaacc gacatatttc ctctgagcac attgaaaaac tgaggtattt
     3061 catcgagtga agtctttcgg atgattttcc ctatgctcgt cactctcgga tcatccttgg
     3121 tagtccagac agtatcggat gcagattttt cctgcacaat catcgaacgg aacttaatca
     3181 tcctgaattt tctgtgatcg agccctattc tttcctggaa aatgaaaaca gggccttttg
     3241 atgtgatttt aatgaaaaaa gcaatcagca gatacaccgg agaaaaaaca atgataaatg
     3301 taagtgaaaa tacaatatcg aataatctct tcagaaaaag attatagccg tatcttactg
     3361 gaatatctcg gatggagatg acaggaattc cgtccattcc ctccactcgt ccccgtgcag
     3421 caataaactc agtaaagccg ggaacgattt tcagttcaat tccctccctg tcacagagat
     3481 caatcgaatc cttgagccag ttcctttcat tgactccaag agcataaatg acaatgtccg
     3541 gtctttcctt cgaaagtata ttttccgagt gccgagtcca gcttattccg gataaattat
     3601 ggaaatgcag atacattc
//
LOCUS       DAOQGJ010000143         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14586_length_3616_cov_3.696995, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000143 DAOQGJ010000000
VERSION     DAOQGJ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14586_length_3616_cov_3.696995"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="PK683_03305"
     CDS             complement(<1..1728)
                     /locus_tag="PK683_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNH07503.1"
                     /translation="MLRLLIISLYLVLSVYCGKSEKISAPEASKVEDVPWEGTEADIP
                     PQMRELNPLASKDAKKGGVIRNYSNQYPKSLNYYLEQFSTTAEIFRMMFEPLISYHPA
                     TLQPIPRLAKSWKISADKKKFRFELDKNAKWSDGKPVTAQDVVFTFQTIMDKKNNTAV
                     FRIELSRFEEPVIIDDYTVEFAAKDNHWNNFDAVGGHLFILPKHYFEGKDFNKINEEF
                     PVISGPYRLAEAKQGRYVRMTRRGDYWQRAYPFNKGRYNFNDIFFKVFSEEAVAFQAM
                     LKGDMDLYPAYKAATWVKEAVGEKFDKFWIVKQRIFNKKPIGFQGWAMNLRRELFKDK
                     RVRQAIAHLVDRRTMVDKFAYNEYDVTNSYYPDYYLGNEKAKNPNPPIEYDLEKARAL
                     LKEAGWKPNAAGILEKNGKEFRMKILDREKSTERYFTFFMESAKKVGIVAELESTDLA
                     GWSARMDKFDFDLTWTAWGGGVFKDPEPMWHSKYAKEEGQHNYPGIEIPAVDALVEKQ
                     KTVFDVEERNRIVKEIDRIIYTEYPYVLLWHLDNTRLMFWNRFGMPENPLGLYGGESF
                     ALEYWWFDEN"
     gene            1821..3242
                     /locus_tag="PK683_03310"
     CDS             1821..3242
                     /locus_tag="PK683_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNH07504.1"
                     /translation="MNLETISDRFFHSYEFKAADFIWFMGVWKPSGASKKIAREHAGL
                     QSEYRKALSDLTDDDIAGSPYSISEYQPNPILCKGMSKIRELKLKLEKQGKKLILDFV
                     PNHMSVDSPLIDQYPDLFLFKEGAETDQNSFLHRNGRVYFHGRDPYFDGWTDTVQWDF
                     SRPGVLTLHRQFLLEIADVCHGVRCDMAMLPMKDVFEKTHGKTAIDYWKPLIDSIRQV
                     YPNFIFMGEVYWNREYDLQQEGFDFTYDKTLYDRLKSGTGYEIRSHLQAEESFQKKSL
                     RFMENHDEERAYHAFGERSIHCFSLLNFIPGAILYHEGQSLGLEIKLPVQLSRASREN
                     SKPEVQEFYKRCFTQLIHRKHSELYREDLTFHPFDSAENLFISFSLSYTTEKVLHQYK
                     TKEYNLEILTFNPESREIAGWLHPGKQSLDILHGIHSDKVIMTDVLTGLTYEKDKKEI
                     FEKGIYVNLKPSYAHWFVVHISG"
BASE COUNT         1020 a          797 c          733 g         1066 t
ORIGIN      
        1 gttttcatcg aaccaccagt attccagtgc aaaagactct cctccataaa gcccaagcgg
       61 gttctcaggc ataccgaatc tgttccagaa catcagccgg gtattgtcca gatgccagag
      121 gagaacataa ggatattctg tatatataat cctgtcaatt tccttcacta tcctgtttct
      181 ctcttctacg tcaaaaacag tcttctgctt ttccacaagt gcgtctaccg caggaatttc
      241 aattcctggg taattgtgct gcccttcttc cttcgcatac tttgaatgcc acatcggttc
      301 ggggtctttg aacaccccgc cgccccaggc tgtccatgtc agatcaaaat caaacttgtc
      361 cattctcgca ctccagcctg caagatcagt actctccagt tctgcaacga ttcctacttt
      421 tttggcagat tccataaaaa atgtaaagta cctttcagta cttttttctc gatccagaat
      481 tttcatcctg aattcttttc catttttctc cagaatgccg gcggcgttcg gcttccatcc
      541 tgcttccttc agaagagccc ttgctttttc cagatcatac tctatcggag gattcggatt
      601 tttcgccttt tcatttccaa gataataatc aggataatag gaattggtaa catcatattc
      661 attataagca aatttatcga ccattgttct gcggtccacc aggtgagcga tcgcctgacg
      721 aactcttttg tccttgaaaa gctcccttct caggttcatt gcccagccct gaaatccgat
      781 cggcttttta ttgaaaattc tctgcttcac aatccagaac ttgtcaaact tttctccgac
      841 tgcctccttg acccaggttg cagccttgta ggcaggataa agatccatat ctcctttcag
      901 cattgcctga aatgcaaccg cttcttcaga aaaaacctta aagaaaatat cattaaaatt
      961 ataacggcct ttattgaaag gataagctct ctgccagtag tcgccccttc tggtcatcct
     1021 gacgtaccgc ccctgctttg cttcagcaag tctgtaaggt cctgaaatga caggaaactc
     1081 ttcattgatc ttgttgaaat cctttccctc aaagtagtgt ttggggagga tgaacagatg
     1141 gccgcccact gcgtcgaaat tgttccagtg gttgtccttt gcagcaaact ctacagtata
     1201 atcatctata ataacaggtt cttcaaacct ggacagttca atccggaaga cggcagtatt
     1261 gtttttcttg tccataattg tctgaaaagt aaaaacaacg tcctgagcag tgacaggttt
     1321 cccgtcagac cattttgcat ttttatccag ttcgaaacgg aatttctttt tgtccgctga
     1381 aatcttccag cttttggcca gccttggaat cggctgcagg gtggcaggat gatatgagat
     1441 cagaggctca aacatcattc ggaaaatttc tgctgttgtg ctgaactgtt ccagataata
     1501 gttcagagat ttcggatact ggttgctgta attcctgatg accccgcctt tcttggcgtc
     1561 ttttgatgca agaggattga gttctctcat ttgaggaggg atatcagcct cagtgccttc
     1621 ccaaggaacg tcttccactt ttgaagcctc aggtgcggaa attttttccg acttgccgca
     1681 ataaaccgat agtaccagat agagggatat gataagaagt ctcaacatat tcagggggag
     1741 tttccttttt gcaaagtaga tatttagatc aagcctttat ttatcaatta aacactaggg
     1801 ttttttgtaa agaaaacaat atgaatctcg aaacaatttc agaccgtttt ttccacagtt
     1861 atgaattcaa ggcggctgat tttatttggt ttatgggagt ctggaaacct tcgggtgcat
     1921 caaagaaaat tgcgagagaa cacgcgggac ttcagtccga gtacaggaag gccctctccg
     1981 atctgacaga tgatgacata gcaggatcac cctattccat ttcagagtat cagccgaatc
     2041 cgattttatg caagggaatg tcaaaaatcc gtgaacttaa gctgaaactc gaaaaacagg
     2101 ggaaaaaact catccttgat tttgtcccaa atcatatgtc agtggattct cctctgatag
     2161 accagtatcc tgacctgttc ttattcaagg agggtgcaga aacagatcag aattcatttc
     2221 tgcacagaaa cggaagggtc tactttcacg gaagagatcc ttactttgac ggatggacag
     2281 acacggtgca gtgggatttt tccagacctg gagtactgac tctgcacaga cagtttcttc
     2341 tcgaaattgc cgatgtctgt cacggtgtca gatgtgatat ggcgatgctt ccgatgaagg
     2401 atgtttttga aaaaacacac ggaaagactg ctatcgacta ttggaaaccg ctgattgatt
     2461 ccatcaggca ggtgtacccg aatttcatct ttatgggaga agtttactgg aacagagagt
     2521 acgatcttca gcaggaagga tttgatttta cctatgataa aactctttat gatcggctga
     2581 agtccggaac cggctatgaa atccgttcac atcttcaggc agaagaaagc tttcagaaaa
     2641 aatccctgcg ctttatggaa aaccacgatg aggaaagggc ttatcatgct ttcggagaga
     2701 ggtcaattca ctgtttcagt cttctgaatt ttatcccagg tgcaatactt tatcatgaag
     2761 gacagagtct cggactggaa atcaaactgc ctgtgcagct cagcagggct tctcgcgaaa
     2821 attcaaagcc tgaggttcag gaattttaca aacgatgctt cacccagctg attcacagaa
     2881 aacattctga gctttaccga gaagatctga catttcatcc ttttgattct gctgaaaatc
     2941 tatttatcag cttttcactg agttacacta ctgaaaaagt tctgcatcag tacaaaacaa
     3001 aggaatacaa tctggagata ctgacattca atccggaaag ccgagaaata gcgggctggc
     3061 ttcatccagg aaagcagtcc ttggacattc tgcacggaat tcactccgat aaagtcataa
     3121 tgaccgacgt tctgacagga ctgacgtatg aaaaagacaa gaaggaaatc tttgagaagg
     3181 gaatctatgt aaacctgaag ccgtcctatg cccattggtt tgtagttcat ataagtgggt
     3241 aaaaaaacag ctgtatttcc attgtttctc tgtcgtaatc ttttatagtc aaataaccag
     3301 tctgaacaag aagtggattc agacttagat tttcgatttc ataactggaa aaaccaagct
     3361 catccaagat gagacttata ttcattatat aataattatt ttgaaatata acattcaaga
     3421 gaaagcttgg tgttcctgtt tcaaaccagt gatgctgaaa ctttctggaa tcgaagagca
     3481 ggagcgtgga aaacggattg ttatactgca gttccggttt ttccggccaa tcaataatag
     3541 gtttatcata ttcatcgatc agtattactc agatttctaa tctcgctacg ctcgattaga
     3601 aatcaaaaat ggtagg
//
LOCUS       DAOQGJ010000144         3611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14624_length_3611_cov_3.392857, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000144 DAOQGJ010000000
VERSION     DAOQGJ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14624_length_3611_cov_3.392857"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PK683_03315"
     CDS             <1..566
                     /locus_tag="PK683_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07505.1"
                     /translation="CSAEDKTVYLDGLKGLIKKNTDFQIKDETVLRNSKTFSSLKMTE
                     EENLLNISGNPLVKESKHKNVFSGILSETDSDQVLLAKIHYNLSHKEISQYMMEFKLH
                     ATLTVLMYDRNGRLIFSSEKSAERQTNYADDKFSVGGLVVDALNLLFNNKTEIPIHKS
                     CRPVLQENLNELWNSTLSDLKKKYPSI"
     gene            563..1138
                     /locus_tag="PK683_03320"
     CDS             563..1138
                     /locus_tag="PK683_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07506.1"
                     /translation="MIFRTVSVPKFLQILHGKEPRLSSIVLTYLGGLIGAAVSASGFV
                     QSSLPLWKICILSTLMLDICGGVIANLSISTNLYYQENRKIRLIFLAVHIFQPAVFAV
                     LIPENAYYFITVFLVTFSSGLLIHFLRDIEQQQNLASFCIAAALPIFALFALSSSVLY
                     AFGPLYMLKLILGFSVRRPSFLEKREDKKDC"
     gene            1174..1905
                     /locus_tag="PK683_03325"
     CDS             1174..1905
                     /locus_tag="PK683_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07507.1"
                     /translation="MILIASYFIFTYNYFDGWWYSCIGTVLIVLISFFIFKSEFLKAA
                     GLKFKLSAVPGISVFLLFAVFCSLTMIEYISRRNGITILSRADWSYVYHAVFYTMNEE
                     IILGSIILYILIHRWKIHPLFSSVLLALCFSVLHFAVYKWIFSEKGILMPAALVTLFF
                     AGILRNNLIIYAGHIGYSWALHFSWIIIMFGKSLRYSEQWGEVSEPERFNIYLGSVEM
                     ALISSVLAVLSSMYGILKNSAQREQ"
     gene            2043..2966
                     /locus_tag="PK683_03330"
     CDS             2043..2966
                     /locus_tag="PK683_03330"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH07508.1"
                     /translation="MKTLIAFISIISLWSCAEKETEFHPKAVVIIRAGDVRVGNRPVN
                     LGDEITDKDTIVLGGKSICDLQVLDADSQVVIRLKEFSKFRLSGKQIGSRKETNFLID
                     AGNAMLNVPKLGKNEDFKAMSPSATAGVRGTKYDVNVGLNGNTRINVLEGAVAMKVHI
                     PEMDKFSNADIRSSKALSAVSESLDAKEIVLEKGKSTEMPKSVGTKIIKETGLGEAVK
                     RDAPKDLDKDVDAKTLEEKMEKIEKEELKLIVKEIDKGTMSQRLQEYEELNPIDKEIV
                     NDKEKRKAVIQARYQKFEGNWLQRLLKFLKF"
     gene            2979..3404
                     /locus_tag="PK683_03335"
     CDS             2979..3404
                     /locus_tag="PK683_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HNH07509.1"
                     /translation="MISIITIFLFLIFLFLSSVHFYWGFGGKWGNGAVIPTKDGTEKV
                     IMPGPVPTFAVGSGLFCIGCIILIKGGLLELSLPYWISKSSLWVVSFIFILRAVGEFS
                     YVGFFKKVKDTKFAENDTRYYSPLCLLIGILTLIIELNR"
BASE COUNT         1090 a          694 c          752 g         1075 t
ORIGIN      
        1 gatgttctgc agaggataaa acagtctatc tggatggact gaaaggtctt ataaaaaaga
       61 acacagattt tcagatcaag gacgaaaccg tacttcgaaa ttccaaaaca ttctcttctt
      121 tgaaaatgac tgaggaggaa aatcttctga atatttcagg aaatcctctt gtaaaagaat
      181 caaaacataa gaatgtgttt tcaggtattc tgagcgaaac cgactcagat caggttctcc
      241 ttgcaaaaat tcattataat ctatcccata aagagatcag tcagtatatg atggaattca
      301 agcttcatgc aaccctaaca gttcttatgt atgacaggaa cggaaggctg atattcagtt
      361 cggaaaaatc agcagaaaga cagacaaatt atgcagacga caaattttct gtaggcggtt
      421 tggtcgtaga tgcactgaat ctgctcttca acaataaaac cgagatccca atccacaaat
      481 cctgccgtcc tgtccttcaa gaaaatctga atgaactctg gaacagcact ctctctgatc
      541 ttaagaaaaa gtatccatct atatgatttt tcggacagta tccgttccga agtttctgca
      601 gatcctgcac gggaaagagc cccgtctctc ctcaattgta ctgacttacc ttggaggtct
      661 cataggtgca gctgtatctg catccggatt cgttcagtca tctcttcctc tctggaagat
      721 atgcattctt tcaacattga tgctggacat atgcgggggt gtcatcgcaa atctaagcat
      781 ttccacaaat ctgtactatc aggaaaaccg gaagatccgt ctgatttttc tcgcggtgca
      841 tatttttcag cctgcagtct ttgctgtcct catacctgaa aatgcatatt acttcattac
      901 agtatttctt gtcacttttt catccggcct gctgattcac ttcttacggg atattgaaca
      961 gcagcagaat ctggcatcat tctgcattgc agcagccctc ccgatatttg cactgtttgc
     1021 gctgagcagt tcagtcctgt atgcattcgg tcctctctat atgctgaagc tcattctggg
     1081 attttccgtc agaagaccgt catttctcga gaagcgggaa gataaaaagg attgctgaat
     1141 ctttataaaa tatcaaaaac tgccttgctg attttgatat taattgcatc ttattttata
     1201 tttacataca attactttga cggatggtgg tattcctgca tagggacagt cctgatcgta
     1261 cttatcagtt ttttcatctt caaaagtgaa tttctgaaag ccgcaggctt gaaatttaaa
     1321 ctcagtgcag ttcccggaat ttcagtattt ctgctcttcg ctgttttctg ttcactgaca
     1381 atgattgaat atatttcccg gagaaacgga ataacgattc tctcccgcgc agactggtca
     1441 tatgtttatc acgcagtctt ttatacaatg aatgaggaaa tcatcttagg ttcaattatt
     1501 ctttatatac tgatccacag atggaaaatt catccattat tttcttcagt tttactggcg
     1561 ttgtgctttt ctgttctcca ctttgcagtt tacaaatgga tattttcgga gaaaggaatc
     1621 ctaatgcctg cagcgcttgt tactcttttc tttgctggca ttttaagaaa caatctgatt
     1681 atatatgcag gacatatcgg ttattcctgg gcactgcatt tcagctggat aatcattatg
     1741 ttcggaaaat ctctccgata ttcggaacag tggggtgagg tttctgaacc ggaaaggttc
     1801 aatatttatt tgggctccgt ggaaatggct ctgatctcat cagtcctcgc cgttctgagt
     1861 tcaatgtacg gaatattaaa gaacagtgcc caaagagaac aataaaagcc tgtctgttga
     1921 aaaaaatttg acaggaaaca ctgagtcatt tttaataatc tacaaatcct gcaattttta
     1981 ctgtcatctg ggaaggtttt ttggatacaa tagcattatt tttttcaata taaggaaaca
     2041 ttatgaaaac actcattgca ttcatttcca tcatttcact ttggagctgt gcggaaaaag
     2101 aaactgaatt tcatccgaaa gctgttgtca ttatccgtgc gggagatgtc cgtgtcggaa
     2161 acagaccggt caatctgggt gatgaaatta cagacaagga tacgattgtg cttgggggga
     2221 aatccatatg cgatcttcag gttctggatg cagattccca ggttgtcatc aggctgaaag
     2281 aattcagcaa attcagactg agcgggaaac agatcggttc caggaaggaa acgaattttc
     2341 tgattgatgc aggaaatgcg atgctcaatg ttccaaagct tggaaaaaat gaggacttca
     2401 aagcgatgag tccttccgca actgccggtg tgagaggaac taagtacgat gtaaatgtag
     2461 ggctaaacgg caatacaaga atcaatgttc tggaaggtgc agtggcaatg aaagtccata
     2521 taccggaaat ggataaattt tccaatgcag atataagaag cagcaaggcc ctttctgcag
     2581 taagtgaatc tcttgatgca aaagagattg tactggaaaa gggaaagtcc actgaaatgc
     2641 cgaagtctgt aggcactaaa ataataaaag agacaggttt gggagaagca gtcaaaaggg
     2701 atgctccgaa agatctggac aaggatgtag atgcaaagac tctggaagag aaaatggaaa
     2761 agattgaaaa agaagaactg aagcttattg taaaagaaat agacaagggc acaatgagtc
     2821 agagacttca ggaatacgag gaactgaatc cgattgataa agaaatcgta aatgataaag
     2881 agaaacgaaa agcagtcata caggccagat accagaaatt cgaaggaaac tggcttcaaa
     2941 gacttctgaa gtttctgaaa ttctaggatt cccaaagaat gataagtatt attacaatct
     3001 ttctgttcct tatttttctc ttcctttcat ctgtccattt ctactgggga ttcggcggca
     3061 aatggggaaa cggtgcagtt attccgacaa aagacggcac tgaaaaagtg attatgcctg
     3121 gacctgtgcc tacgtttgct gtaggttcag gtctcttctg cataggatgc attattttaa
     3181 ttaagggagg tctgcttgaa cttagtctgc cctattggat cagcaagtcg agtctttggg
     3241 tcgtctcatt catttttatt ctgagagcag tcggggaatt cagctatgta ggtttcttca
     3301 agaaagtcaa agatacaaaa tttgcggaga acgatacaag atactactct ccgctctgtc
     3361 ttctgattgg aatattgact ttaatcattg aattgaacag ataaagttaa gtgttcaaac
     3421 agaccattat tttttctgac ttcatatctc aaaatcatta tactatcttc tcgagaggga
     3481 gttcgaattc aggaagaaca tcttctccgc tgagtattct gtcaaatccg ctcagttcct
     3541 ctcaggagac tttcctttcc tgtaaacaat ggtttttttc tgaaatggat caatcagcca
     3601 ggcgagagaa c
//
LOCUS       DAOQGJ010000145         3610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14636_length_3610_cov_3.022504, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000145 DAOQGJ010000000
VERSION     DAOQGJ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14636_length_3610_cov_3.022504"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1849)
                     /locus_tag="PK683_03340"
     CDS             complement(53..1849)
                     /locus_tag="PK683_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/class IV aminotransferase"
                     /protein_id="HNH07510.1"
                     /translation="MQIQFTEWDDYILSQCENEEYLFLFESSLSDGSDKSTYIFKNPA
                     ASLEFDSFFELKKYVSSCSSHRMQVFAVPYEFGYASYPERTERYKTDLIRVFEFDRVE
                     EISFEKRRKMHSIFTEQTEFPFDSRIVSECRKTDYTEKIKSILREIQEGFVYQVNFSF
                     PLKFEIAEGTEYPLYSVLRRFQPTDYNAFYKMKNKFILSLSPELLFEEKGRSAVMRPM
                     KGTASLQEARSMIHEKHKAENFMIVDLLRNDLGRVCEFGSVRINNLLQIENYRLISQL
                     TSEIEGKIRKDSDLCDLLQAVFPSGSVTGAPKLEAMKVIDRLESSARGIYTGALGFNN
                     LKKRVFSVAIRTVEVTEGRCRYRTGSGIVYDSVPEMEYEECFLKAGILFKALDLRFFE
                     SVLFRKGRFIFLNDHFERLRKSFRLFFGEDLSFEKDFLPFLNEKAKFLNNKLDYKIRI
                     EISGSRKVRTLSEEVIIRKGKTDLKKICLSETKVSSFEIMLQHKTNHRQFYDQMLSEV
                     RENGFFESVFLNERDEITEGCFTNIFCRIDGIYYTPPSDSGLLAGVFRDRLLKRFPKI
                     FRQRKLYIQDMISSDSIFICNSVRGIIRVRYE"
     gene            complement(2034..3359)
                     /locus_tag="PK683_03345"
     CDS             complement(2034..3359)
                     /locus_tag="PK683_03345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNH07511.1"
                     /translation="MKLNQEIALCSPRRTPFAQIGKSLSLYPSHHLGKIVAEDILEKT
                     KLPKTEIDGVIVGEGFANAPNSARVIMNLMGLPDEIPALTVSNNCVSGMEAVVEAARR
                     IALGEGDLFLCIGEESQTAMPFIVKGARLNKKTATVDKLKAALPDSLPEGVELRDTLE
                     DGLGDGETSYGMQVTAEILSQNYGLSREINDKVAFQSFKRAYEANEKGLYKDYIISVK
                     DDEGNPLQADEAVILRKGLVENPGRMAKAMLLFENPQFKFEEFKTKYSKYLKKSHGPT
                     LSIFNASPRSDGAAGVIVCTVEKAKKLGLKIDAVLTGWRMKGVDPNLMGLGQALSTLG
                     LLEDMKLTMNDIDQIEIHEAFAATAVAALEEIKAKTGFDWEKKFDEGKINAFGGSIAI
                     GHPFGATGIRLISNAVMDFRQFPETDRVLLTACAHGGVAGSLMIERYKG"
BASE COUNT          981 a          846 c          677 g         1106 t
ORIGIN      
        1 tttttcattc tgcagaccgt ctctcggccc aacttcagta attctgatct ttttattcat
       61 atctgactct tattattccc ctgactgaat tgcagataaa tatgctgtcg gatgaaatca
      121 tatcctgaat gtacagtttc ctctgacgga agatttttgg aaatcttttt agaagacggt
      181 ccctgaatac gcctgcgagc agacctgaat ccgaaggggg agtataataa attccgtcta
      241 tcctgcagaa tatatttgta aaacagcctt cagtgatttc atctctttcg ttcagaaaaa
      301 ccgactcaaa gaatccgttt tctctgactt ccgaaagcat ctgatcataa aactgtctgt
      361 gatttgtctt gtgctgaagc attatttcaa atgaagagac ctttgtttca gacaggcaga
      421 ttttttttaa atcagtcttt ccctttcgaa taatgacttc ttcggacaga gttctgactt
      481 tcctgcttcc ggagatttca attctaattt tataatctaa cttattattt aaaaactttg
      541 ctttttcatt caaaaaagga agaaagtctt tttcaaaaga gagatcctcg ccgaaaaaaa
      601 gacggaagct ttttcgaagt ctctcaaaat gatcattcag aaagataaac cggcctttcc
      661 tgaaaagaac agattcaaaa aaacgaagat caagagcctt gaaaagtatg cctgccttca
      721 gaaaacattc ttcatactcc atttcaggca ctgaatcata tacaattccg cttcctgttc
      781 tgtatctgca tcttccctca gtcacttcca cagttctgat tgctacactg aatactcttt
      841 ttttcagatt gttgaatccc agcgcacctg tgtatattcc tctggcagaa gactccagtc
      901 tgtcgatgac tttcattgct tccagcttgg gagcacccgt gactgagcct gaaggaaaaa
      961 ctgcctgaag cagatcgcag agatcactgt cttttcttat tttcccttca atttcagaag
     1021 tcagctgaga gataagtctg taattttcaa tctgcagaag gttgttgatt ctcacagaac
     1081 cgaattcaca tacacgtccc agatcatttc tgagcagatc cacgatcata aaattctctg
     1141 ccttatgctt ttcatggatc atggaacgtg cttcctgcag actggcagtg cctttcatcg
     1201 gcctcatgac tgccgatctc cccttttcct caaaaagcag ttcaggagaa agagacagga
     1261 tgaatttatt cttcatttta taaaaagcat tataatcagt gggctgaaac cttctgagaa
     1321 ccgagtacag cggatactct gttccctccg caatttcaaa cttcagagga aaggaaaaat
     1381 tgacctgata gacaaaacct tcctgaattt ccctgagaat acttttaatc ttttctgtat
     1441 aatccgtctt tctgcattca gatacaattc tgctgtcaaa cggaaactct gtctgctcag
     1501 tgaatatgga atgcattttt cttctttttt caaaagagat ttcttccact ctgtcaaatt
     1561 caaaaacacg gatcagatca gtcttgtatc tttcagttct ttcaggataa gaggcataac
     1621 cgaattcata cggaactgca aatacctgca ttctgtgaga actgcaggaa gagacatact
     1681 ttttcagttc aaaaaaactg tcaaattcca gggatgctgc cgggttctta aaaatataag
     1741 tagacttatc agatccgtct gaaagagagc tttcaaaaag aaacagatat tcttcatttt
     1801 cacattggga aaggatgtag tcatcccatt cagtaaattg aatctgcatt taaaggaagg
     1861 tatgcagtgc tgaaccgtac tgctttctga aaaagtcatc tgagctttga agatcaccgg
     1921 gaacgcagat tggaaatgaa aaaatcatat tggattctgc ctttctactt ctttcgatct
     1981 tcacggtcta tgagtttcgg agcctatgcg gccccgaaat taccgggatc aaatcagccc
     2041 ttgtaccttt ctatcatcag agaaccggcc actccgccgt gagcgcaggc agtgagaaga
     2101 accctgtctg tttcaggaaa ctgtctgaaa tccattacag cattggagat cagtcggatt
     2161 cctgtggcac cgaaaggatg ccctattgca atagaacctc cgaaagcgtt gatcttgcct
     2221 tcgtcaaatt tcttttccca gtcaaatcca gtcttagcct tgatttcttc aagagctgca
     2281 actgcagtcg cagcaaaggc ttcatgaatt tctatctggt ctatatcatt catggtaagc
     2341 ttcatatctt ccagaagtcc cagggtagag agtgcctgtc cgagtcccat cagattcggg
     2401 tcgactcctt tcattctcca gcctgtgaga actgcatcaa ttttcaatcc gagctttttg
     2461 gctttttcca cagtgcatac aatcactcca gctgctccgt cagaacgcgg gcttgcattg
     2521 aagatgctca gtgtaggtcc atgcgatttt ttcagatact tggaatactt agtcttgaat
     2581 tcttcaaact tgaactgagg gttttcaaag agaagcattg cttttgccat tctgcccgga
     2641 ttttccacca gccctttcct gagaatgaca gcctcatcag cctgaagagg atttccttca
     2701 tcatctttaa cactgataat ataatcctta tataatcctt tttcattagc ttcataggct
     2761 ctcttgaagc tctgaaaagc aaccttgtca ttgatttccc tggaaagtcc gtagttctga
     2821 gacagaattt ctgcagtcac ctgcatcccg tagctcgtct ctccgtcgcc cagcccatcc
     2881 tccagagtgt ctctgagttc cactccctcc ggcagactgt ccggcagtgc agctttcagc
     2941 ttgtcgacag ttgcagtctt cttgttcagt cttgctccct ttacaatgaa aggcatggct
     3001 gtctgagact cttcaccgat gcagagaaaa agatctcctt ctccgagtgc gattcttctt
     3061 gcagcctcga ccactgcttc cattccggaa acacagttgt tggatacagt cagtgcagga
     3121 atctcatcgg gaagacccat cagattcata atgacccttg cagaattggg tgcatttgca
     3181 aagccctcac ctacgatcac accgtcaatt tccgtcttgg gaagctttgt cttttcgagg
     3241 atgtcttccg caacaatctt tcccaaatga tgacttggat agagtgaaag tgattttccg
     3301 atttgtgcaa acggagttct tctgggagaa cagagtgcaa tttcctgatt cagtttcata
     3361 atgacctcat ttctctgcat tgcagggatt ttgactactt ctaagactca agttgaaaac
     3421 cgagggacag aactgcaagc aaaattttcc atcggtaaat tatgcgtccg gcagaacaat
     3481 attccggaaa tactccgcac tttcttcagg aattgcagag ttaatgtaca tcccgctccc
     3541 catttcaaat cctgcctgct tgctcatttt cggaatgata gaaagatacc agtgaaagta
     3601 tcttgtctcc
//
LOCUS       DAOQGJ010000146         3601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14693_length_3601_cov_3.655668, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000146 DAOQGJ010000000
VERSION     DAOQGJ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14693_length_3601_cov_3.655668"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..442)
                     /locus_tag="PK683_03350"
     CDS             complement(56..442)
                     /locus_tag="PK683_03350"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNH07512.1"
                     /translation="MKKETAKKYSDDVLKFISRLESPWDQLFLEMRNLIVETLPEFEE
                     SLKYGTPFYTHKGLLCYLSLKGKKEKYLDFGLCQGFKIQGFEDVLLFQGRNTVKSVRI
                     DSKKDIQKELFQKILQEAWMVNEFKE"
     gene            complement(452..1744)
                     /locus_tag="PK683_03355"
     CDS             complement(452..1744)
                     /locus_tag="PK683_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNH07513.1"
                     /translation="MGSKQNRVGFILNQAVILSVFFCGACSSNAPVPLPQKKSVSPKE
                     IFQKNSSSVPVIHSYVEDRHTGNGSSFVANYNSRNYLITNYHVITAGYFAVVHNGKII
                     KNYSVLRANDIEDIAVLTFPGIENISGILISEYEPSEGEGAFAMGYPVITTSEQASLT
                     IAEGLVSNSRLIFNRGEIGTKRYIQLSVPVNPGNSGGPIFNDQGSLIGMTTLKWINLS
                     SVSAAIPNADIIREIKAAVPDTESLKKEEAKTGIMKRISLIAVSLNELDVLEFGYHYS
                     PSVKLGIHGEVKESAERIRAAVTAAGEKYPNDREQYNRFLSEALSPSDYLFFLMTMRY
                     FQEHREANLDSVLGSPFLASQLYLAGRFYFLFTKMADAPAGDIVLKKYTVTKVKFNKG
                     RTKALVEMDVETASKTFPAKLEFIREWGNWFLLPTYSL"
     gene            1875..2645
                     /locus_tag="PK683_03360"
     CDS             1875..2645
                     /locus_tag="PK683_03360"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH07514.1"
                     /translation="MKQKKFLVLLIPALLLAAFLLFRGNGSEKVFIKPRKSDVIEAVY
                     GLGTVSAAKIFHLKIGVMSQIRKIFVEEGQEIRSGQPLVSFDSIPAIPSPISGIVTVI
                     TYKENEIAFPQMNILTVMDVSEKYITLSLEEQSVIRVRKGQKARIALEALKDRTFTGE
                     VKSVYPGDNQFYIRISSKELPAEVLPGMTADIAIETGAKSNVFQVPEKSVRGGKITVR
                     KDGKTSEIKVKTGLKTDGWIEITEPILSGDEEFLSEKN"
     gene            2645..>3601
                     /locus_tag="PK683_03365"
     CDS             2645..>3601
                     /locus_tag="PK683_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH07515.1"
                     /translation="MFFIAFRQMTARKMQTFLTLLGIVFGSMGYIVISGVMLGFREYL
                     LEQLVNNDAHVKVSPRLEIITREGTEKLLFDQKENISWISEPAGRKDNSEIRSASHWY
                     NLLSNDPDVEAFSRQLASKVIITKGSLTDAGSVIGVEPLKHSRVTNIRDNMLYGKFTD
                     LGDSGNKLIMGEGLREQVGIRINESVYLSAGPGKSMPFKVIGSFRTGNKAIDDNVCYA
                     SLNDVQTLNRTPGKISSISIRLKDVSLAQEKAAYYLSTNPDRVESWDQANASFLTIFK
                     LQDTIRYAITVIILVVSGFGIYNILNILINQKKREIAILRSIG"
BASE COUNT         1019 a          813 c          799 g          970 t
ORIGIN      
        1 atcaaggtgg gcggtgaaaa tcactcggta acctgctttt acacagtccc gcagatcatt
       61 ccttgaactc attcaccatc caggcttctt gaaggatttt ctgaaacagt tccttctgaa
      121 tgtccttttt ggaatcaatt ctgaccgatt ttacagtatt tcttccctga aacaggagaa
      181 cgtcttcaaa gccctgaatc ttaaacccct gacagagtcc aaaatccaga tatttctctt
      241 tttttccctt cagagacaga taacagagaa gtcctttatg agtgtagaaa ggtgtcccgt
      301 acttcaggct ttcttcaaat tcgggaagag tctccacaat cagatttctc atttccaaaa
      361 aaagctgatc ccagggagac tcgagcctgg aaatgaactt cagtacatcg tcagaatatt
      421 ttttagcagt ttccttcttc ataggcagat ctcagagact gtatgtaggc agaagaaacc
      481 agtttcccca ttcccgtata aactcgagtt ttgcaggaaa tgtcttgctt gctgtttcca
      541 catccatttc cacaagggcc ttggttcttc ccttgttgaa tttaaccttg gtcacggtat
      601 attttttcaa tacaatgtct cctgcaggag catccgccat ctttgtgaac agaaaataaa
      661 atcttcctgc aagatagagc tgggatgcaa gaaagggaga tcccagaact gagtccagat
      721 ttgcttccct gtgttcctga aaatatctca ttgtcatcag gaaaaaaaga tagtcggaag
      781 gactcaatgc ctctgacaga aatctgttgt actgctccct gtcattcgga tatttttctc
      841 cggcggcagt gactgcagca cggattcttt ctgcggattc ctttacctca ccgtgaattc
      901 cgagtttcac tgaaggagaa taatggtatc cgaattccag tacatccagc tcattcagac
      961 tgactgcgat gagactgatt cttttcatga tccctgtttt tgcctcttcc ttcttcagtg
     1021 attccgtatc gggcactgcg gccttgattt ccctgataat gtccgcattc ggtattgcgg
     1081 cactcactga ggaaagattg atccacttca gtgtggtcat cccgatcagg ctgccctgat
     1141 cattaaatat aggtcccccg ctgtttccag gattgacagg aacagaaagc tgaatatacc
     1201 ttttggtacc gatttctccc ctgttgaaaa tcagtctgct gttgctgact agaccctcgg
     1261 caattgtaag acttgcctgt tcagaggttg taatgacagg gtatcccatt gcaaatgcac
     1321 cctctccctc gctcggctca tattccgaaa taagtattcc ggagatgttt tctattccgg
     1381 gaaaggtcag aactgcaatg tcctcgatgt catttgctct cagaactgaa tagtttttaa
     1441 tgatttttcc attatgaacg accgcaaaat aacctgcagt gatcacatga tagtttgtaa
     1501 tcagatagtt tctgctgttg taatttgcaa caaaagaaga cccgtttcct gtatgtctgt
     1561 cttccacata actgtgaatg accgggacag aactggaatt tttctgaaat atttccttgg
     1621 gggaaactga ttttttctga ggcaggggaa caggcgcatt ggaggaacag gccccgcaga
     1681 aaaaaacgga cagaatgacc gcctgattca gaataaatcc gacccgattt tgtttagatc
     1741 ccatatatag cccctgccct gaaaagaatg aagaggacag gttctgctta caagtcattt
     1801 tttaaggaaa tctgaaaatg cggagtcttc cgcaaaattc tttcgttcta tgtacagtaa
     1861 aataaactgg ctttatgaaa cagaaaaagt tcctcgtgct tctgattccg gctctccttc
     1921 ttgcagcctt tcttcttttc agagggaacg gatcagagaa agttttcatc aaacctagaa
     1981 agtccgatgt cattgaagct gtttacggtc taggcaccgt gagcgctgca aagatttttc
     2041 atctgaaaat cggagtgatg agccagatca gaaaaatatt cgtggaggaa ggccaggaaa
     2101 tcagatcagg acagcctctg gtgagctttg attccattcc tgcaataccc tccccgattt
     2161 ccgggatcgt gactgtgatc acttacaagg aaaatgaaat tgcatttcct cagatgaata
     2221 ttctcacagt catggacgtg tctgaaaaat acatcacgct ttctctggaa gaacagtcag
     2281 tcatcagggt gcgcaagggt cagaaggcaa gaatcgctct ggaggctctg aaagacagga
     2341 ctttcaccgg agaggtcaaa tcagtatatc ccggagacaa tcagttttat atacggattt
     2401 cctccaaaga acttcctgca gaggttctgc cgggaatgac tgcggacatt gcaattgaaa
     2461 cgggagcaaa atccaatgtg ttccaggtgc ctgagaaatc ggtcaggggc ggaaagatca
     2521 ctgtgagaaa agacggaaag acatctgaga tcaaagtaaa gacaggtctc aagacggacg
     2581 gatggattga aatcactgaa cccatcctct ccggcgatga agaatttctt tcagagaaaa
     2641 actgatgttc ttcattgcat tcagacagat gactgcaaga aagatgcaga catttttgac
     2701 tcttctcgga atcgtattcg gttccatggg atatatcgtc atttcaggag tgatgctcgg
     2761 attcagagaa tatcttctgg aacagctggt gaacaatgat gcccacgtaa aggtttcgcc
     2821 cagactggaa atcatcacca gagaaggaac ggagaagctt ctgtttgatc agaaggaaaa
     2881 catttcctgg atctccgaac ctgcaggcag aaaggacaac agcgagatcc gctctgcatc
     2941 tcactggtac aatctgcttt ccaatgatcc ggatgtggaa gcattctcca gacagcttgc
     3001 atccaaggtc atcatcacaa agggaagtct gacggatgca ggaagcgtca tcggtgtgga
     3061 acctctgaag cacagcaggg tgacaaacat acgggacaat atgctgtatg gaaaatttac
     3121 tgatctcgga gacagcggaa acaaactcat catgggcgag ggtctcagag aacaggtagg
     3181 gatacggatc aatgaatcag tttacttaag cgcaggcccc ggaaaaagta tgcctttcaa
     3241 ggtgatcggc tccttccgca caggaaacaa ggcaatcgac gacaatgtat gttatgcttc
     3301 actgaacgat gtgcagaccc tgaacaggac tccgggaaaa atcagctcca tctccatccg
     3361 tctgaaggat gtctctcttg cacaggaaaa ggcggcatac tatctcagca caaatcctga
     3421 cagggtggaa agctgggatc aggcaaatgc cagttttctc acaatattca aactccagga
     3481 cacaatccgt tatgcgatca cagtcatcat tctggttgtc tccggattcg ggatatacaa
     3541 tattctgaat attctcatca atcagaagaa aagggaaatt gcaattctgc gttccatcgg
     3601 g
//
LOCUS       DAOQGJ010000147         3574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14859_length_3574_cov_3.235862, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000147 DAOQGJ010000000
VERSION     DAOQGJ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14859_length_3574_cov_3.235862"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2109
                     /locus_tag="PK683_03370"
     CDS             <1..>2109
                     /locus_tag="PK683_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07516.1"
                     /translation="FQPAEIASPINYAVARQSLEILELLLTHGANPNFTTSGIITTLG
                     QACVNGRLDMVELLLDWGAKVDGVYSAEKKTALMIAAESGREDIVEALLYAKADPNLK
                     DKTGKNALEYAQAKDHSLVVSILAPLTAGANAEAEPLIKESDLIGQEIAKHSLKQALA
                     LAIVNEERKKHSLPTFKVNLHAVFTGSSGTGKTTFARYYAQEIKKIGILRSGHIVEVS
                     RLDLVGEYIGQSAPRTADAVERARGGILFIDEAYSLKIDKNDTPGQEAINTLIKFMED
                     YRNELVVILAGYTGMMRSFLDLNPGLKSRIPNVIEFDDFTDEQIGKLLDDMCTKHEMQ
                     MDEKDRTFAIEQVLIKKRGKGFGNAREVRNIFERTAAQHCVRLSNQDLKSLPKETHQK
                     FIYSDITVDPFDDGTRSGLQPSDPKENPKSGIYRLHALRGMKGIKEEIQAMADFIRIR
                     KLRKGASSARGLQLHMVFTGNPGTGKTTVARLIGDIYRDLGVLPSGHLIEVDRSGLVG
                     GYLGQTALKTKECIEDARGGILFIDEAYSLFSESHSGDMYGREAVNTILKYMEDYRDE
                     VVIIFAGYQEPMNKLMNSSPGLLSRFSKTLLFNNFSDEELSDICKDICANDGYDITES
                     AHKKLVSQLLERRKTDPDFANARTARNLIEQAFKNHAQRIIQLNPRDLMNRAVLNTIE
                     EADIHGLPPLSSLNMRKIGFD"
     assembly_gap    2110..2209
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2239..>3574
                     /locus_tag="PK683_03375"
     CDS             2239..>3574
                     /locus_tag="PK683_03375"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH07517.1"
                     /translation="MILKNIVRKISEIFMYGSEFAESSQAKRKVKLSNIVSFIVLFTN
                     TSYIPVLHSLNLSHIQHVLVAGSFIYICVPILNRFGFTVFSRICLLSNMVVILLYYSY
                     VLGKHSGVYVMFIAFSSLPFVFFEFKNRVEVIFSASAGLICFIVFEYFDRTRTAQGLL
                     QGKEAEYLAYSIFFSTYLVIFAVVLSLEFTNTRAENELILLKESQDGDYFLTTQIISP
                     LFKNLNSSDYIQTDFLMKQKKSFVFKGRKRELGGDICITGNLNFRGTEYTMFINADAM
                     GKSMQGAGGAIVMGTVINSILSRNAGRTINVQPKQWLTSTYNELQNVFIEFDGSMMIS
                     CFLGLISTRSGKMLYLNSEHPSSVILRDEKAFFLEDSPIRKIGFPGSEENIHISEYQL
                     YPGDIIISGSDGRDDINLTPLEKTRKINEDEKLFLSIVEKSKGDLQIIYRELN"
BASE COUNT         1043 a          683 c          821 g          927 t
ORIGIN      
        1 tttcagcctg cggaaattgc atctccgatc aattatgctg ttgccaggca gtccctggaa
       61 attctggaac ttcttctgac tcacggtgca aatcctaatt ttaccacatc cggcataatc
      121 acgactctgg gtcaggcctg tgtaaacggc agactggata tggtggagct tcttctggac
      181 tggggcgcaa aagtggacgg ggtttattca gccgaaaaga aaacggcttt gatgattgcc
      241 gctgagtcag gcagagagga tattgtggaa gctcttcttt atgcaaaggc ggatccgaat
      301 ctgaaagaca agaccggcaa gaatgcactg gagtatgctc aggcaaagga tcattctctg
      361 gttgtatcca ttcttgctcc tctcactgca ggcgcaaatg cggaggctga acctctgatc
      421 aaggaatcgg atctgatcgg gcaggaaatt gcaaaacact ccctgaagca ggccctggca
      481 ctagcaatcg taaatgagga acgcaagaag cattctcttc ctacattcaa ggtcaatctt
      541 catgcagttt ttaccggaag ttccggtaca ggaaaaacta cgtttgccag atactatgca
      601 caggagatta aaaaaatcgg aattctccgt tccggtcata ttgtggaagt ttccagactg
      661 gatctggtag gagagtacat agggcagtct gcacctagga cagcggatgc agtggaaagg
      721 gcgagaggcg gaattctttt catagatgaa gcatacagtc tgaaaattga taaaaatgac
      781 actcccggtc aggaagcaat caatactctt atcaagttta tggaagacta tcgaaatgag
      841 cttgtagtca ttctggcagg gtacaccgga atgatgcgca gttttctgga tctgaatccg
      901 ggactgaaaa gccgtattcc gaatgtaatt gagtttgacg attttacaga cgagcagatt
      961 ggtaaacttc tggatgatat gtgtacaaag catgagatgc agatggacga aaaggacaga
     1021 acctttgcga tagagcaggt tctgatcaaa aaaagaggca agggtttcgg aaatgcgaga
     1081 gaggtgagaa atattttcga gcgcacggca gcacagcact gtgtgcggct ttccaatcag
     1141 gatctgaaaa gtcttcccaa ggaaactcac cagaaattta tttacagtga tatcacagtg
     1201 gatccgtttg atgacggaac aaggtccgga ctccagcctt cagatccgaa ggaaaatcca
     1261 aagtcgggaa tttacagact tcatgctctt cgaggaatga agggaatcaa agaagagatt
     1321 caggcaatgg cggattttat ccgtatccgc aaactgagga agggagcttc ttctgcaaga
     1381 ggacttcagc ttcatatggt ctttactgga aatcccggaa caggcaagac aacggttgca
     1441 agactgatcg gagacatata cagggatctg ggagttcttc ccagcggtca tctgattgaa
     1501 gtggacagat ccggtcttgt agggggttat ctgggacaga cagctctcaa gacaaaggaa
     1561 tgcattgagg atgcaagggg cgggatactt tttattgatg aagcatattc tcttttcagt
     1621 gaatcacatt caggagatat gtacggaagg gaagcagtca atacaattct aaaatatatg
     1681 gaagattaca gagatgaagt tgtcattata tttgcaggat accaggaacc gatgaacaaa
     1741 ctgatgaatt ccagccctgg ccttctcagc cgtttcagca agaccctcct gttcaataat
     1801 ttttcagatg aggaactgag tgatatctgc aaggacatct gtgcaaatga cggttacgac
     1861 attactgagt ctgctcataa aaaactggtt tctcagcttc tggaaagaag aaaaacggat
     1921 cctgattttg caaatgcaag gacagcccga aatctgattg agcaggcatt taaaaatcat
     1981 gcacagagaa tcattcagct gaatcctaga gatctgatga acagggcagt tctgaacaca
     2041 attgaggagg cagacattca cggacttcct cctctcagtt cactgaatat gagaaagatc
     2101 ggctttgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agacagttga
     2221 ccttaaagga tcttgaaaat gatactcaaa aacatagttc ggaaaatcag tgaaattttc
     2281 atgtacggtt cagagtttgc tgaatcgagt caggcaaaga gaaaagtaaa actttctaat
     2341 atagtctctt ttatagtatt gtttacgaat acgtcctata ttcctgtgct gcattcactg
     2401 aatctttcgc acatacagca cgtactcgta gcaggttcct ttatttatat atgcgtgccg
     2461 attctaaaca gattcggatt cactgttttt tccagaatat gcctgctttc caatatggtt
     2521 gtcattcttt tatattacag ttatgttctt ggaaaacatt caggtgtata tgtgatgttc
     2581 attgcattct cctcacttcc ttttgtgttc tttgaattca agaacagagt tgaggtgatt
     2641 ttttccgctt ctgcaggatt gatttgtttt attgtgtttg aatattttga ccgcacaagg
     2701 acagcacagg gacttcttca gggaaaggaa gcagagtatc ttgcctattc catatttttt
     2761 tccacctatc tggttatatt tgcagttgtt ctgagcctcg agttcacaaa tacaagggca
     2821 gaaaatgagc tcattcttct gaaggaaagt caggacggag actatttcct cacaacacag
     2881 attatctctc ctctgttcaa gaatctgaat tccagtgatt atattcagac agactttctg
     2941 atgaagcaga aaaagtcttt tgtattcaag ggaagaaaac gggagctggg aggagatatc
     3001 tgcattacag ggaatctgaa tttcagagga acagaataca ctatgttcat caatgcggat
     3061 gcaatgggca agtcaatgca gggggccgga ggagcgattg tgatgggaac agtcatcaat
     3121 tccatccttt ccagaaatgc aggcaggaca atcaacgtgc agccgaaaca gtggctgaca
     3181 agtacataca atgaactgca gaatgtattc attgagtttg acggatccat gatgatctcc
     3241 tgtttccttg gactcatcag cacacgttcc ggaaaaatgc tttacctgaa ctcagaacat
     3301 ccttcgagcg tcatcctgag agatgaaaaa gccttctttc ttgaagacag tccaattcgg
     3361 aaaatcggtt ttccaggctc ggaggaaaat attcatatca gtgaatacca gctctatcct
     3421 ggcgacatca ttatttccgg aagcgacgga agggatgata tcaaccttac ccctcttgaa
     3481 aagaccagaa aaatcaatga ggatgagaaa ctgtttttaa gcattgttga aaaatccaaa
     3541 ggagatctgc agatcattta cagggagctg aatg
//
LOCUS       DAOQGJ010000148         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14875_length_3571_cov_3.462173, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000148 DAOQGJ010000000
VERSION     DAOQGJ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14875_length_3571_cov_3.462173"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PK683_03380"
     CDS             complement(<1..713)
                     /locus_tag="PK683_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07518.1"
                     /translation="MDNLQQLSMDAVKAVLQSSSIEELTMREIYDFFQKEDSLSNYKP
                     ESVFQIDPRNGELIVYNSSRATRPHRTDSEPKQVLPENACPICSGKTTGIMDIAELSS
                     GFTFINKNMFPIFFPFDDDPHNRMGSVSFGLHFLQWTSSVHTHDWHNMPVKDLKIVMQ
                     RLAALERKLLLESGSLMPTSEFSSEEKPTYGYVSIIKNYGKIAGGSLAHGHQQIVASN
                     IMPKQFYNNWNFYVSHKLN"
     gene            816..1403
                     /locus_tag="PK683_03385"
     CDS             816..1403
                     /locus_tag="PK683_03385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNH07519.1"
                     /translation="MNDDSELKIEPSRIAQDWHHKNCYGCGPDNLKGLNQEFPFDEKT
                     GDVLFITKIENSFEGAPGFAHGGVLASILDEAQGVLCFHIGHFVMTDQLNINYFKASP
                     LNEDLEIRAYITSVRKRRLYTTAEIRLVRTGELLCKSKAKWYDMPERIIRKMFHRSPL
                     SVEKLIRIMEENKKRGKQIRKRLKTEESKNSSKVE"
     gene            1828..3456
                     /locus_tag="PK683_03390"
     CDS             1828..3456
                     /locus_tag="PK683_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH07520.1"
                     /translation="MKIKGDLYSEIIQGKDIIADVLPPPEYIIESQLYAYQLLSEKRK
                     NEAPAIIAKLGKLKKDYYDRNQYWKTVLEESELKKTLTADSFQYADQFFQILDSEFIP
                     NILNNNYILSEKILSEKLVPLYEKHREAVDKTVVIAENLNRKNEQNAEEFIWKGRMFL
                     FLAALFSILISFSLALFTNMKMLVLPLKNLLKAVRKMEDGDLQVELNLNEFENTKDEL
                     ILFSQSLYSVIEKIKLVLRKNQDLVKALLKSSGEISSSSDSFNDSIQSQSVSSEEISA
                     AIEEIGAGIRAVNSFSKKQNTDLKNFETELSLFFQLVENLGRHINSQTVISEKIAADS
                     VKNTGSMKQMNESLQNILKSSRDMSSISEIIKNISKQVNLLALNASIEAARAGESGKG
                     FSVVADQISSLAEKTASSIRKIDSILENNSIEIRNGIAYSEAAIKTALNTQSGISSMA
                     KLIQEEYRIIQEHQSINKNVQSGMQNMENLSDQIEQSMQEHLKAIEDIEESMNNIIEK
                     TQKNSIASEEINLRSSALLHLSQDLDAEIRFFKV"
     gene            complement(3469..>3571)
                     /locus_tag="PK683_03395"
     CDS             complement(3469..>3571)
                     /locus_tag="PK683_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNH07521.1"
                     /translation="PQRIGHIFPFEKAREALELFKSGTTVGKVVLKV"
BASE COUNT         1190 a          684 c          682 g         1015 t
ORIGIN      
        1 aagttcagct tatggcttac atagaaattc cagttattgt aaaactgctt tggcataata
       61 ttgctggcaa caatctgctg atgaccgtgg gcaagagatc ctcctgcaat ttttccgtag
      121 tttttaatga tggatacata tccgtatgtc ggtttttctt cggatgaaaa ttcagatgta
      181 ggcataagac ttccggattc cagcagaagt tttctttcca gtgcagcaag cctctgcatc
      241 acaatcttta agtccttcac aggcatattg tgccagtcat gcgtatgcac tgacgaggtc
      301 cactggagaa aatgcagtcc gaaagaaaca cttcccattc tgttgtgagg atcatcatca
      361 aacggaaaaa agatcggaaa catattttta tttataaaag taaatcctga actcagttca
      421 gcaatatcca ttatacctgt tgtttttccg ctgcaaatcg ggcaggcatt ttcaggaaga
      481 acctgtttcg gctccgaatc agtccgatga ggtcttgtgg ctctggaaga attatagaca
      541 atgagctctc cgtttctcgg atctatctga aatacagact caggcttgta attgctcagt
      601 gagtcttctt tttggaagaa atcataaatt tctctcattg tcagttcctc aatcgaactg
      661 gattgaagaa ctgcctttac tgcatccata ctcagctgct gtaaattgtc catatctttc
      721 aattctcgtt ttgcaaatat tccgtgtcaa gaaccttttt aaagcggaaa tggatttaga
      781 ttgaaggtat tgcaggcatt aaaaccattg aatcaatgaa tgatgattct gaactgaaaa
      841 ttgaacctag ccgaattgcg caggactggc accacaaaaa ctgctacgga tgcggacccg
      901 acaaccttaa aggcctgaac caggaatttc cttttgatga aaaaacaggg gacgttctgt
      961 tcataactaa aattgaaaac tcttttgaag gagctccagg gtttgctcac ggaggagttc
     1021 ttgcttccat tctggatgaa gctcagggag ttctctgttt tcatatcgga cattttgtaa
     1081 tgacagatca attaaatatt aattatttca aggcatcacc tctgaatgaa gatctggaga
     1141 tccgtgctta cataacctca gttagaaaaa gaagacttta cactacagca gaaattcgtc
     1201 tggtcaggac cggcgaactg ctctgcaaat ccaaggcaaa atggtatgat atgccggaaa
     1261 gaatcatccg caaaatgttt caccgctcac ctctttctgt ggaaaaactg atcaggatta
     1321 tggaagagaa taaaaaacga ggaaagcaga tacgcaaaag attaaaaaca gaagaatcca
     1381 aaaactcgtc aaaggttgaa tagacatatt ataaaaaaca gcactaaata taagtgattt
     1441 gggaggcagt gttttctcat tgtctctgca gattatcata acttttcttt gccttaaaaa
     1501 ataagcatta aatttaaatg tttgtacatt aaatctgcct gaatcataca gatttctaat
     1561 tcgcttttct ataaaacttc ctttcgaaaa tactgattct cattttcaaa atatgagcaa
     1621 tatattttat cataatctca acgggagacg aaaaaatatt gacaattttt ttctgcagat
     1681 cattctcata tgaaattttt cggaaggtaa tccgtgttcg attatttatt tagacagaaa
     1741 gttctgatta aagttatttt ctcactctgt atcctgatga ccggattcct tttttacatt
     1801 gcatactcat cttatattct ggaaaggatg aagattaagg gtgatctcta ttctgaaatt
     1861 attcaaggaa aagacatcat cgccgacgtt ctgcctccgc cggagtacat catagaatca
     1921 cagctctatg cttatcagct tctcagcgag aagaggaaaa atgaagctcc tgcaatcatt
     1981 gccaaactcg gaaaattaaa gaaagactac tatgacagaa atcaatactg gaagactgtt
     2041 ctggaagaat ctgaactcaa aaagacactg actgcagatt catttcagta tgccgatcag
     2101 tttttccaga ttctggattc tgaatttatt ccaaatatac tgaataataa ttatatatta
     2161 tcggaaaaaa ttctatcaga aaaacttgtc cctctgtatg aaaaacacag agaagctgtg
     2221 gacaaaacgg tagtcattgc agaaaattta aaccggaaaa atgaacagaa tgcagaagag
     2281 tttatctgga aggggagaat gtttcttttt cttgccgcgc tcttttccat actgatcagc
     2341 ttttcactgg cactgtttac aaatatgaaa atgctggttc tgcccttaaa aaaccttttg
     2401 aaggccgtca gaaaaatgga ggacggcgat ctgcaggttg agctgaatct gaatgaattt
     2461 gaaaacacca aagatgaact gattctgttc agtcagtctc tttattctgt cattgagaaa
     2521 atcaaacttg tactcagaaa aaatcaggat cttgtcaagg ccctgctgaa atcttccgga
     2581 gaaatttcat ccagttcaga ttctttcaac gactcaatac aaagtcagtc agtatccagt
     2641 gaggaaatat cggcggcaat tgaagagatt ggagcaggca ttcgtgcagt aaattcattc
     2701 agcaaaaaac agaatacaga ccttaaaaat tttgaaacgg agctgagtct tttttttcag
     2761 ctggtcgaaa accttggcag acatataaac agccagactg tcatcagcga aaagatagct
     2821 gctgattcag taaaaaacac cggatcaatg aaacagatga acgaaagtct tcagaatata
     2881 ctgaaaagct ccagagacat gtccagtata tcagaaataa ttaaaaatat atctaaacag
     2941 gtaaatcttc ttgcactgaa cgcttccatt gaggccgcac gagctggcga aagcgggaaa
     3001 gggttttctg tggtagctga tcagatttcc agcctggcag aaaagactgc atccagtatt
     3061 cgaaaaattg attctatcct cgaaaataac agcatcgaga ttcgaaacgg gattgcatat
     3121 tcggaagcgg caatcaagac cgcattgaat acacagtcag gaatcagttc aatggcaaaa
     3181 ttgattcagg aagaatacag aatcattcag gaacatcaga gcataaacaa aaatgttcag
     3241 agcggaatgc agaatatgga aaatctgtca gatcagattg aacagtcaat gcaggaacac
     3301 ctgaaagcaa tagaagacat tgaagaatca atgaacaata tcatcgaaaa aactcaaaaa
     3361 aattcaattg catcagaaga aatcaatctg agatcctcag ctcttctcca tctctcgcag
     3421 gatctggatg ctgaaatcag attctttaag gtataattat ctgatatatt atacctttaa
     3481 tacaactttt ccgacggtgg ttcccgactt aaaaagctcc agtgcctctc ttgctttttc
     3541 gaaaggaaaa atatgaccga tcctctgagg c
//
LOCUS       DAOQGJ010000149         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14900_length_3566_cov_3.373968, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000149 DAOQGJ010000000
VERSION     DAOQGJ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14900_length_3566_cov_3.373968"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1230)
                     /locus_tag="PK683_03400"
     CDS             complement(49..1230)
                     /locus_tag="PK683_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH07522.1"
                     /translation="MYRLHSASKSGNLETMKYLIETGADINAKDSNGRIPLHYASFAG
                     DLEKVKLLTQKGAKIRDKDNENSTSLHFASYFGKLEIIQYLIEQGAEVNVKDNSASTP
                     LHYASQLNDNFKTVKYLIEKGAEVNAKDEYGQTALHKACGASIDTVKYLAEKGADIDL
                     RDNGGNTALHYFSISGNLQTVKFLVKKNARVYAKNNLGRTPLHNASGWGKIEIVDYLI
                     EKQAKLNAKDIENRTPLHLASVVFGNVKIVKSLAEKGANLNAKDKNGDTPLHRASAVF
                     NLETVRVLIKTGAAINIKNNDGLTPLSSAISDMETVKYLVTKGADVNTKGNDGETPLF
                     YAIRYGNLDTVKYLVEKGADIHIKDNTGNTPLHYAAESGNSDIAKYLIEKGADINAKK
                     Q"
     gene            complement(1650..1856)
                     /locus_tag="PK683_03405"
     CDS             complement(1650..1856)
                     /locus_tag="PK683_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HNH07523.1"
                     /translation="MNRKSVTSKRISSIGYENSTLEVEFRNRSIYQFSPVPEMIYLSF
                     LNAPSKGSFLSTQIEGNYFYRRVC"
     gene            complement(2024..3124)
                     /locus_tag="PK683_03410"
     CDS             complement(2024..3124)
                     /locus_tag="PK683_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07524.1"
                     /translation="MNKKQILKKASAFSVSLALLFAMSCKSEDKNKGTTQKLGIIYLW
                     QSQMCARNNPFPNGGSVTSAVNVSPSPASGSDYNYGYLNAGGADYVIHKKTIQKISST
                     GALSDFAGSTSSTASTDGTGTSAGFVSITSWAADSAGNIFVIDTISLSTSLALRKITP
                     AGVVTTPVTYTASGLNRISVYVAIDSSDNIYIYTRNANTDAVTIQKATTSALSFSAYY
                     SSSDPTFYYSSSYGNTMFADKKGNLYIMTSSGLQILNSSLQLAKASFASSFGSSTSLQ
                     FDSSGNAYFNGSVGNDRGIVKADSSGNTTMLLRGTSAIGMACNTPGYGAYGINLKSTG
                     EIQGYGNMAGSYYGLNAAVDYTKYYLFTYKQP"
BASE COUNT          994 a          698 c          719 g         1155 t
ORIGIN      
        1 ggtactgcgg gatgttttat ttctccccat tctgatgcag tgaagccttt actgtttttt
       61 tgcatttata tccgcgccct tttcaataag gtattttgct atgtctgaat ttccagattc
      121 agccgcataa tgaagaggag tgttgcctgt attgtccttt atgtgaatgt ctgctccttt
      181 ttcgacaaga tactttactg tatctaaatt tccatatctt atagcataaa atagaggtgt
      241 ctcgccatcg ttgccctttg tatttacatc cgctcccttg gttacaagat atttcactgt
      301 ttccatatca gaaatcgcag atgaaagagg agttaaccca tcattatttt ttatatttat
      361 tgcagctcca gttttaatga gaacccttac tgtttccaga ttaaacacag ctgaggctcg
      421 gtgcaaagga gtatctccat ttttatcttt tgcattcaaa ttagctccct tttctgccaa
      481 ggattttact attttgacgt ttccgaaaac tacggaagca agatgaagag gagtgcggtt
      541 ttcaatgtct tttgcattta gcttcgcttg tttttcaata agataatcga ctatttcaat
      601 ttttccccac ccggaagcat tgtgcaaagg agtgcgtcca aggttatttt tagcatatac
      661 tcgagcattt tttttaacaa gaaactttac agtttgcaag ttcccactga tggaaaaata
      721 atgtaaagct gtatttccgc cattatctct taagtctata tcagcccctt tctctgctaa
      781 atatttcaca gtgtcaatac tagctccgca agccttatgc agagctgttt gaccatattc
      841 atcttttgca ttaacttcag ctcctttttc aatgagatat ttgactgttt tgaaattatc
      901 gttaagctga gaagcgtaat gcagaggcgt actggctgaa ttatctttca cattaacttc
      961 agctccttgt tcaatcaggt attggattat ttctagtttg ccaaaataag aagcaaaatg
     1021 cagagaggtg ctgttttcat tgtccttgtc ccgtattttt gcgcctttct gagtcagaag
     1081 ttttaccttt tccagatcac cggcgaaaga tgcataatga agaggtatgc gtccattgct
     1141 atcttttgca tttatatctg ctcctgtttc gataaggtat ttcatagttt ccaaattgcc
     1201 ggatttagaa gctgaatgca ggcggtacaa ccttgcttgt ccttctcatt gatatcggct
     1261 cctgtcaaaa tcagaagttt tactttttcc agatcaccag cggacgaagc tgtattcagt
     1321 gtttccgcga aatttcgaga attcaggaga aatgaaaaaa gaagaaacag aaagattttc
     1381 ataaatttat tatcggtgat aaagggacag agtgaacgtc tttcgaatat aaaaactgtc
     1441 attccattta taacttttca tggactcaaa aaaaggagaa cagaggacag tatgagcaag
     1501 ttatgcggga ttctcaaaga cagtagtcaa gaatgcttaa taaattattg ctaatctctg
     1561 cagacgcatc cgcaattatc ggactccgtt ttccggaatc ttgttttagg actgtttaaa
     1621 agaatcctgc gattcaggag aggactgaat cagcagacac gtctgtaaaa ataatttcct
     1681 tcaatctgag tgctcaggaa acttcctttg gaaggtgcat tcaaaaaact cagataaatc
     1741 atttcaggaa ctggagaaaa ctgatagatg cttctgtttc taaattccac ttccagtgtg
     1801 ctgttttcgt aaccgatgga tgaaattcgt tttgaagtca ctgattttcg attcattaaa
     1861 ccagtttttc gccgtaccgg attatgtcta gtaaaacggt cctcatttac aaaaggaaat
     1921 ttgaaaattt ttgttcggat aatactgaag gccttgccat cggctcaggt tgaaaaaaag
     1981 gacagttttg accctgtccc attttatatg cgcacttaaa gaattaaggc tgtttatatg
     2041 tgaagagata gtactttgta taatccactg ccgcattcaa gccgtagtaa ctgcctgcca
     2101 tatttccata cccttgaatt tctcctgtgg atttgaggtt tatcccatat gctccatatc
     2161 ctggagtatt gcaggccatt cctatggcgc tggttcccct taataacatt gttgtattcc
     2221 ccgaggaatc tgccttgaca ataccgcggt catttccgac tgaaccgttg aaataagcat
     2281 ttccagagga atcgaactga agagaagtgc ttgatccaaa actgctggca aacgaggctt
     2341 ttgccaattg aagactgctg ttaagtattt gcagtccact ggaagtcatt atatataggt
     2401 tccctttctt gtctgcaaac attgtatttc cataactgct gctgtagtaa aatgtagggt
     2461 cggaactgga atagtaagcg gaaaaactca atgcagacgt tgtggccttc tgaatggtca
     2521 ctgcatccgt gtttgcgttc cttgtataga tataaatgtt gtcggaactg tctattgcaa
     2581 cataaaccga tattctgttc aatccactgg cagtgtaagt gactggcgta gtgacaacgc
     2641 ctgcgggtgt gatttttctc aaggccagcg aggtagatag cgaaattgta tcgattacaa
     2701 aaatatttcc tgcagagtct gctgcccagg atgttataga aacaaaaccg gcagacgttc
     2761 ctgttccgtc agtgctggca gtggaactgg tgcttcccgc aaaatcactc agtgccccgg
     2821 tggaactgat cttctgtatg gtctttttat ggatcacata atccgcaccg ccagcattca
     2881 gataaccata gttatagtcg gaaccggatg caggacttgg gctgacgttg actgcagacg
     2941 ttactgatcc accgtttgga aatggattgt ttctggcaca catttgagac tgccaaaggt
     3001 aaatgattcc taacttctgg gttgtgcctt tgtttttatc ttctgatttg caggacattg
     3061 caaacagaag tgcgagtgaa acagaaaaag cagatgcttt tttcaaaatt tgttttttgt
     3121 tcattatcat aattctcctt aatgaaaaaa aaaacctatg tgtcaaataa aaaaactgga
     3181 ctttttaaat tttgttttgc agtagagaac tgcgctgatt agctttggat gcccaataag
     3241 ttgaaaatga ctttctatat gtggataaga cgaagtatat gcgcactttc attgaacagg
     3301 ggaaaggcac atattttctt tcacgtccta gaaggttcgg caagagtctg cttctgaaat
     3361 tgagtaagac tgaaaaaaca gtaataccca gatttctaat tagattataa taggatcaat
     3421 gaaatattgg tttaaaatca gttctggtcg acactgaatg tcgccttgca ggctagcaag
     3481 ggcggggatt ttagcggctt caatgttagc gaaggggctg cttaagcccc tttaaaccaa
     3541 tgccggtagg catccctacc attttt
//
LOCUS       DAOQGJ010000150         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14912_length_3565_cov_2.913390, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000150 DAOQGJ010000000
VERSION     DAOQGJ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14912_length_3565_cov_2.913390"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..838
                     /locus_tag="PK683_03415"
     CDS             152..838
                     /locus_tag="PK683_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH07525.1"
                     /translation="MQILIADDEPGIRKFIRIFLNSNEYSTIEASSAMEAASKASEQK
                     PDLVLLDLNFPDQSGFETLKRIREWSQMPIIILSVMNGEDEIIQALDLGADDYVVKPF
                     RAGELLARIRSCLKRKVQEPEPILQCGRLKINFQLRTVYVEEKEVHLTAREYSLLQIL
                     ARKKGGVVLQSQLLKAVWGQEHEKDTHYLRIYIAQLRRKIEINPSEPDILITELGVGY
                     RLRGDYIRGR"
     gene            902..1069
                     /locus_tag="PK683_03420"
     CDS             902..1069
                     /locus_tag="PK683_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07526.1"
                     /translation="MSENNEKKNKKIAKMSLTELDAASKKAKDSGNLQTTSKYLQHIE
                     QRKEELKARGK"
     gene            1072..2985
                     /locus_tag="PK683_03425"
     CDS             1072..2985
                     /locus_tag="PK683_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNH07527.1"
                     /translation="MLQFINIRHQYGPNVLFDGFSWHIKNSCKIALIGPNGAGKTTLF
                     QFAAGTLHPDSGEVVRSKNTAISLFHQIPVFEQNVSVIENVLSSIKHYKIYLNEKHRI
                     DKRFETTDPDSKEFEKLLSDQSHLEEKAHTEGLHDLENNAKKILSGLGFTNAAVERPI
                     KDFSPGFQHRAGLAICLLNPHSLLLLDEPTNHLDDSSKEWLASYLQEIKTTFVLVTHD
                     PDFLNRTTDTIAEISSKGVFEFKGTLEEFLEEKNEVHEKMQLQFKKEEDYIKKRMEWI
                     DRFRAKATKAKQAQSALKRLEKRDKVDRPEDIFWNKKPDYKFNYSSSGKIAFRLEDCS
                     YSYSSSDRKIFEHASMEVSAGEKIALVGPNGAGKSTLMKCIAGLFQISSGNLYFGPKT
                     ERGYFSQTHHDELDGNLNLLQTVQKKYPDLSDVACRTALGHFSFSGDSVYKSVSLLSG
                     GEQSRLRMALLVLQPCSLLLMDEPTNHLDMVTRDALKRSLADFPGAVVIISHDPDFLK
                     LLCTRTFELSGGKLKDLNCSFEDYMQYHKEGVYEQEKLFVKEKTAANYQERNQERNRQ
                     KKIQKDLEKLESDLNELEKQKKSLEVKMADPGFYQTKDYKTELELYETLKLQIDEITE
                     KWALLSEEVTAVS"
     gene            complement(3086..3358)
                     /locus_tag="PK683_03430"
     CDS             complement(3086..3358)
                     /locus_tag="PK683_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNH07528.1"
                     /translation="MEEENLSIQSIAEEMGISSRTLQLRLKREGKSFTYIVQEARESL
                     AEKYLLNNYPVDEISYILGFSEPSVFRRAFKKWTGVTPKEFRKKHI"
BASE COUNT         1151 a          676 c          747 g          991 t
ORIGIN      
        1 gtcttggcct tgggctttcc acatccagag gtcttgtaga cattcaggga ggaacactca
       61 tctgtgatcc tgattcagat gaaacaagat ttatcatcac gcttcctttg gacgaggcag
      121 tcattcctcc cgatcagctg gagaaataga aatgcagata ctgatagccg atgatgaacc
      181 tgggataaga aagttcatca ggatttttct gaattccaat gaatactcta cgatagaagc
      241 ttcttctgcg atggaagctg cgtctaaggc ttcggaacag aagcctgatc ttgttctgct
      301 ggatctgaat tttccggatc agtccggttt tgagacatta aaaagaatca gagaatggag
      361 tcagatgccc attattattc tttctgtaat gaatggagaa gatgaaatta ttcaggcatt
      421 ggatcttggt gcggatgatt atgtagtgaa accctttaga gccggggaac ttctggcaag
      481 aataagatcc tgtctgaaga gaaaagttca ggaacctgaa ccgattcttc aatgcggaag
      541 actaaaaata aattttcaac tgagaacagt atatgtagaa gaaaaagaag tccatctgac
      601 tgcaagagaa tactcacttc ttcagattct tgccaggaaa aagggagggg tcgttctgca
      661 gtctcagcta ttgaaggctg tctggggaca ggagcacgaa aaggacactc attatctgag
      721 gatttatata gctcagctca gaagaaaaat tgaaataaat ccatccgagc cggatatact
      781 gatcactgaa ctgggtgtgg gatacaggct gcgaggagac tatattcggg ggagataatt
      841 taagcaacat tgtacttgac agcctgcctc tttaaaaatt tctaaacata gtgaggtaac
      901 tatgtccgaa aacaacgaaa aaaagaataa aaagatcgct aaaatgagtc tgaccgagct
      961 ggatgcagct tctaaaaaag caaaagacag cggaaatctg cagactacaa gcaaatatct
     1021 tcagcatatt gaacagagaa aggaagaact gaaagctcgc ggtaagtaag attgcttcaa
     1081 ttcatcaata tccgtcatca gtatggaccc aatgtgctgt ttgacggctt ttcctggcat
     1141 atcaaaaaca gctgtaaaat cgccctcatc ggcccaaatg gtgcagggaa aacgacactc
     1201 tttcaatttg ctgctggaac actgcaccct gattcagggg aagtagtcag atccaaaaat
     1261 actgcgattt ccctttttca tcagatcccg gtatttgaac agaatgtctc agtcatagaa
     1321 aacgttctat cttcaataaa acattataaa atatatctga atgagaaaca cagaatagat
     1381 aaacgttttg aaactacaga tcctgattct aaagaatttg aaaaacttct ttccgatcag
     1441 tctcatctgg aagaaaaagc gcatacggaa ggtctgcatg atctggaaaa caacgccaag
     1501 aaaattcttt ccggtctggg ttttacaaat gcagcagtgg aaagaccgat taaggatttt
     1561 tcccccggat ttcagcacag agccggtctt gccatctgtc ttctgaatcc tcacagtctt
     1621 ctgcttcttg acgaacctac aaaccatctg gatgacagtt cgaaagaatg gctcgcatcc
     1681 tatcttcagg aaattaagac aacttttgtg ctggtcactc acgatccaga ttttctgaac
     1741 agaacaacag atacaattgc agaaatttcc tccaagggtg tttttgaatt caagggaact
     1801 ctagaagagt ttctggaaga aaaaaatgaa gttcatgaaa agatgcagct tcagttcaaa
     1861 aaagaagagg actacattaa gaagagaatg gagtggatag acagatttcg tgcaaaggca
     1921 acaaaggcaa aacaggccca gagtgcactg aaacgtctgg aaaaaagaga caaggttgac
     1981 aggcctgaag atatattttg gaacaaaaaa cctgattata agtttaatta cagttcatcc
     2041 ggaaaaattg ccttccgtct ggaagactgt tcctattcct attcttcatc agacaggaaa
     2101 atttttgaac acgccagtat ggaagtttct gcaggggaaa aaattgctct ggtaggtccg
     2161 aacggagccg gaaaatctac tttaatgaaa tgtattgccg gtctctttca gatttcttca
     2221 ggaaatcttt atttcggtcc aaagacggaa agaggatatt tttcacagac tcatcatgat
     2281 gaactcgacg gaaacctgaa tcttcttcag acagtgcaga agaaatatcc tgatctttct
     2341 gatgtcgcct gcagaactgc tcttgggcat ttttccttca gcggagattc tgtttacaag
     2401 tcagtatctc ttctttccgg aggagaacag agccgactca gaatggcact tcttgtgctt
     2461 cagccctgca gtcttcttct tatggatgaa cctactaatc atctggatat ggtgacaaga
     2521 gatgcattga aaagatctct tgcggacttt cctggtgcag ttgtaatcat cagtcatgat
     2581 cctgactttc tgaaactgct ctgcacaagg acatttgaac tttccggagg aaaactgaag
     2641 gatctgaact gcagtttcga agattatatg cagtatcaca aagaaggcgt ttacgaacag
     2701 gaaaaactct ttgtaaagga aaagactgct gcgaattacc aggaaagaaa tcaggaaaga
     2761 aacagacaga aaaagattca gaaagatttg gaaaaattag aatctgattt gaatgaactg
     2821 gagaagcaga aaaaatcact ggaagtcaag atggctgatc ctggattcta ccagacaaaa
     2881 gattacaaaa cagaacttga actgtacgaa accctgaaac tgcagataga tgaaattaca
     2941 gagaaatggg cacttctttc ggaagaagta actgcggtca gctgattcct tttacatcaa
     3001 ttccaattgt ttccgctact ccggctagtt ctttatcatg ggtcagaaga aaaaactttc
     3061 ctgtgatgag agaaattgta caagactata tgtgtttttt tctgaattct ttcggtgtaa
     3121 cccctgtcca ttttttaaat gccctcctga aaacactcgg ttctgaaaat cctaaaatgt
     3181 aagatatctc atcaaccgga taattgttga gaagatattt ttcagcaaga gattccctag
     3241 cttcttgtac tatatatgta aagctttttc cttccctttt aagccggagc tgaagtgtac
     3301 ggctgcttat tcccatctct tcagcaatgc tttgaatgga tagattttct tcttccaatt
     3361 ttttaatgat cagttcgctg actttttgta tgaatgcact ggaaggttca aaagatttca
     3421 atacatcttc agcatatttt tcaaagtaat ttaggagtaa ctgattggaa tgattaattt
     3481 tttttttaag ttccgtgcag aaaaaaaaat tcttgtttct tcagcattga aatgaactgg
     3541 gcattgaaat actctttggt attct
//
LOCUS       DAOQGJ010000151         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14937_length_3562_cov_3.420302, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000151 DAOQGJ010000000
VERSION     DAOQGJ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14937_length_3562_cov_3.420302"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PK683_03435"
     CDS             complement(<1..238)
                     /locus_tag="PK683_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07529.1"
                     /translation="MFEEIQTELLSHPNFYQNISEALVLSLYFMPTFLNRFRLDILAE
                     EETVFVEVSLENSHQEILELKLPEFKSEILDLEIL"
     gene            complement(333..806)
                     /locus_tag="PK683_03440"
     CDS             complement(333..806)
                     /locus_tag="PK683_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07530.1"
                     /translation="MFKESVLHSAEIFEFLRGFIILNTVLLPVSVLTVLFIGIMGGGS
                     PEKMSPWKSMGISAGFVYGLPSGVLAFCLGTGKVLDFIFGINSLSSVSVSWFTVCIAS
                     VVLTFLAEVFVDNSFQLKKGNYKIIIYGILAVMIFMISAYFSGRIPLPWISGWFR"
     gene            1232..2341
                     /locus_tag="PK683_03445"
     CDS             1232..2341
                     /locus_tag="PK683_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH07531.1"
                     /translation="MESFHWNNYYVTDIESVDTQHHRLVDLINEFGNSILDKNNVLEE
                     DLKRLFNSLADYSKFHFSDEEAMMIQAKMDPRHLKYHFKEHTGFLTEVTQLFANISPD
                     NLEPARFLLNFLVHWLAYHILGSDQSMAKQLKMIQSGSTPEQAFLIEEQMRSAATEPL
                     LNALNGLFQQVSERNAQLYNLNRTLEDKVAERTKELLEANQKLEDIAMTDVLTGLPNR
                     RFAMKQFELLWKESETYGHALSCMMIDADYFKQINDSYGHEAGDTVLKDLSRQLQDST
                     RNDDIVCRLGGDEFLIICPNTPLEGIIYLAEQLRSEVESLRIKAGNGEWKGSVSIGVA
                     AKKTGIQSIDELIRAADKGVYIAKGKGRNSVGYIQ"
     gene            complement(2374..2844)
                     /locus_tag="PK683_03450"
     CDS             complement(2374..2844)
                     /locus_tag="PK683_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNH07532.1"
                     /translation="METKLKKKVYEKGDTPLVFPKFENEEKMTVSQICCACTGCCRYV
                     STVIDKPRSKVTIDQYKWFLLHKNVQIYIDNEGDWNLLFITPCTKLQSDGTCGIYETR
                     PDICKDYSPDSCSRTGKDYTHLFATPDALIDYLEEEKAKRKAKKEQKGKKQAGR"
     gene            complement(2829..3320)
                     /locus_tag="PK683_03455"
     CDS             complement(2829..3320)
                     /locus_tag="PK683_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07533.1"
                     /translation="MNTGEAKISKFTFQSDDLNVSFDVRTVDEKNVVRTYWSKFTTKK
                     MLINPSSGSRLEGKIAALSYDLFPDEENGQGFEVIEALTKGKDAAPAKETAVLDFLFE
                     NGKKVQIKFFIKTDINGANYYPVIRKTDDFTESPAYMKENSLRELADTVKQIKDEPEW
                     KQN"
BASE COUNT         1101 a          745 c          743 g          973 t
ORIGIN      
        1 gcagtatttc cagatccaga atttccgatt tgaactcagg aagcttcagt tccagaatct
       61 cctgatgcga attctccaga ctcacttcga caaaaacagt ttcttcttct gccagtatat
      121 ccagccggaa acggttgagg aatgtcggca taaaatataa tgatagaacc aaagcctcac
      181 tgatgttctg ataaaaattc ggatgactca gcagttcagt ctgtatttct tcaaacataa
      241 gaatgaattt ttattttgtt tttcaatgta aagaaatttt taaaaaatca agcagattga
      301 gtaaaaaccc tgcttccgaa aaaccttcgg agctacctga accagccgga aatccaaggc
      361 agaggaatcc ttccggaaaa ataggcagag atcataaaaa tcattactgc cagtattcca
      421 tagataatta ttttataatt acctttcttc agctggaagg aattgtccac aaagacttcg
      481 gcaagaaagg tcagaacaac ggaagcgatg cagacagtaa accagctgac cgaaacggat
      541 gacaaagaat tgattccgaa aatgaaatcc agaaccttgc ctgtccccag acagaatgcc
      601 aatactccgg aaggaagccc gtaaacgaag cccgcagaaa ttcccatcga tttccaagga
      661 ctcatttttt caggagatcc tcctcccatg attccaatga acagaacagt cagtacggaa
      721 accggcagca gaacagtatt gaggatgatg aatccgcgca ggaactcaaa tatttcggcg
      781 gaatgcagaa ctgattcttt aaacatgata gaattgactg tatgcaggag gatatcttca
      841 gcaattcttt tttgctgaat gtacttcttc tgaaaaaaag aaagttatga atatacagag
      901 aaagtcagtt ttgactgatg catctgaaag taaaaaactt gattctttct gaaacgagtc
      961 agacgcattt caattctgag catcagtatc agaaagacaa tcctgtctgc ctgaaaaaat
     1021 atactgttat acagaaagat acaaaatctc acaataaagc attgattgaa aacaggtcat
     1081 tgaaaaattg aaagtgatca gagagcagcg cattcgatgg ccgtacaaac tttcataatc
     1141 tgtgcggcag gagtcttaaa ttgaaccgga tttgctgcca gtcagtctaa cagacagttt
     1201 gaaagacgtc ggtcgggtta aaaaggattt tatggaatca tttcattgga ataattatta
     1261 tgtaaccgac atagagtcgg tagacacaca gcaccacaga ctggtagatt taatcaatga
     1321 attcggaaac agcattctgg acaagaacaa tgttctggaa gaagatttga aaagactttt
     1381 caacagcctg gctgactatt ctaaatttca tttctctgat gaagaagcaa tgatgataca
     1441 ggcaaaaatg gatccgagac atttaaagta tcattttaag gaacataccg ggttcctcac
     1501 agaagtcact cagctgtttg caaatatcag tcccgacaat ctggagcctg ccagatttct
     1561 gctgaacttc cttgtgcact ggcttgcata tcatatcctg ggttccgatc agagcatggc
     1621 gaagcagctg aaaatgatcc agtccggaag cactcctgaa caggcttttc tgattgaaga
     1681 acagatgaga agcgcagcga cagaacctct cctgaatgcc ttgaacggtc tttttcagca
     1741 ggtgtctgaa cgcaatgctc agctgtacaa tttaaacaga acactggaag acaaagtggc
     1801 tgaaagaaca aaggaacttc tggaagccaa ccagaagctg gaagacattg ccatgacaga
     1861 tgtactgacc ggtctaccca acagaagatt tgcaatgaaa cagttcgagc ttttatggaa
     1921 agaatcggaa acttacggtc acgccttatc atgcatgatg atcgatgcag attattttaa
     1981 gcagatcaat gacagttacg gacatgaagc aggagacact gtactgaaag atctgtccag
     2041 acagcttcag gattccactc gaaatgatga tattgtatgc aggctcggcg gtgatgaatt
     2101 tttaattatc tgtccaaata ctcctctgga aggaattatt tatctggcag aacagctgag
     2161 aagcgaagtc gaaagtctca ggataaaggc agggaacgga gaatggaaag gaagcgtaag
     2221 cattggagtt gctgcaaaaa aaacgggaat tcagagcatt gatgaactga tcagggctgc
     2281 agacaagggt gtctatattg ccaaaggaaa aggacgaaac tctgtaggct atattcagta
     2341 aataaaggcg gtgccgaaac acctcctgta aagttacctg cctgcctgtt tctttccctt
     2401 ctgttccttt ttggccttgc gttttgcttt ctcctcttcg agataatcga taagagcgtc
     2461 aggcgtggcg aagagatggg tatagtcttt tcctgtgcgg gaacaggaat caggtgaata
     2521 atccttgcag atgtcaggac gcgtttcata gattccgcag gttccgtcgc tctgaagttt
     2581 ggtacacggc gtaatgaaaa gaagattcca gtctccttca ttgtcaatat agatctgcac
     2641 attcttgtgg agcagaaacc atttgtactg atctattgtg actttgctcc taggcttgtc
     2701 aatcacagtg gagacatatc tgcagcaccc tgtgcaggca cagcagatct gagagacagt
     2761 cattttctct tcattttcaa acttgggaaa tactaaagga gtatctccct tttcgtagac
     2821 ctttttcttc agttttgttt ccattcaggt tcatccttga tctgttttac agtatccgca
     2881 agctcacgga gagaattctc tttcatatat gcaggcgact ctgtaaaatc atctgttttt
     2941 ctgatcacag gataataatt tgcaccgtta atatctgttt ttataaaaaa cttgatctgc
     3001 acctttttcc cgttttcaaa aaggaaatcc agcacagccg tttctttggc tggcgcagca
     3061 tctttgccct ttgtcaatgc ttcaatcact tcaaaaccct gtccgttctc ttcatcagga
     3121 aaaagatcat aggaaagggc agctatcttt ccttccagtc tggaaccgga gcttggattg
     3181 atgagcattt tctttgttgt aaacttgctc cagtatgttc tgactacgtt cttttcatcg
     3241 actgttctca catcaaagga cacattcaga tcatctgact gaaaagtaaa cttggatatt
     3301 ttagcctctc cagtattcac cagagtcttt tcacgaagac cgaatacagg atcataaaat
     3361 ttctctgcaa tataattatc cagtgatgcg agagtcagat tatgaagaat cagtctgccg
     3421 ttcttttccc tattttcctt tccgaaaaga aaactctctt ccttctttcc cccataatac
     3481 agttctgcag atgcagaaga agcaggaaga gccgaagagc gcgctgttct cgccgtacga
     3541 cggcctcacg atctgcgacg ga
//
LOCUS       DAOQGJ010000152         3559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14954_length_3559_cov_3.479737, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000152 DAOQGJ010000000
VERSION     DAOQGJ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14954_length_3559_cov_3.479737"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PK683_03460"
     CDS             complement(<1..667)
                     /locus_tag="PK683_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07534.1"
                     /translation="MEKCISSPFSQPVPLKTGFFRSVRSKFIQSICCIKQLSRNSPKF
                     IRILCCKLKSSGFPPGTNKKIPLQSAFFYSININKFINIFNKTALSAIFLIFPETHSR
                     EIPVSLEETLTLSGSSRILSRIQEGKKEVTKLIMREKWREYLPKAGINYFSIRNLNQN
                     QNDSLYQDIRVSLNQLIYDGGENFRAVETAKLNELLNEQDFRMNLFKNRLDVRRVYIR
                     AIVG"
     gene            complement(755..1363)
                     /locus_tag="PK683_03465"
     CDS             complement(755..1363)
                     /locus_tag="PK683_03465"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNH07535.1"
                     /translation="MLIDLHPKNPEIRTLREISEKLKAGAVYIFPTDTVYAMIADPVS
                     KTGVEKLYFLKEIPKNKPLSLLCYDISQVSNYVEYVPNTAYRLMKRITPGPFTFILKA
                     SKTLSKYNYSHTKNKSIGVRICDHIFIRTLLEIHGTALVSTSVFRDDEYIIDPKDLED
                     HFGKRCDGIVDDGIKTLEFSTIVDFTSDEMEIVREGKGFDLI"
     gene            complement(1593..1889)
                     /locus_tag="PK683_03470"
     CDS             complement(1593..1889)
                     /locus_tag="PK683_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07536.1"
                     /translation="MNNDEKKIVRLADKIKNIEENNRFLDEWLFKHKKKYPESAIDIH
                     LEKVLGFILLNDYKTAYKIIRERTLKKDSGGFVSGMKNFYDSAAEYSELFVNAS"
     gene            complement(2274..2594)
                     /locus_tag="PK683_03475"
     CDS             complement(2274..2594)
                     /locus_tag="PK683_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07537.1"
                     /translation="MRVCFLAVCIILAGCKKKYINEDIPECDSFGNYYENGEHYISRE
                     FLTESGSLDYKKLFDKSRQQNSNCYPTETFREPANSGTDPLIQNLPSDKPSPEPNPTL
                     PKQN"
     gene            complement(2591..>3559)
                     /locus_tag="PK683_03480"
     CDS             complement(2591..>3559)
                     /locus_tag="PK683_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07538.1"
                     /translation="LLISSGLSSQAAPAKTDASAAPKKETAPAVSGKTDASPSADKKE
                     DTKPAKKETGTVTVDPQAIANTLKNTNLKHLKKLKSAFYNNAMEEKYNSAMKGYVDST
                     ITLSERDFVLARRKFEQNYTDMNESAKSLIDKYKAVYAQLHTDYSVKVVELKIKGSGN
                     ELNNSTFEKLIAMANEQQNNAIDQASKNNHIEALYLYKKAILNLLKIPYYIERNANKN
                     LKINERLSKNLLIDEDYYPKDHLKTYDDCMDQIYEEKEKLREKEREDERKRINSRLGK
                     AADESPKKDTKASKDSTTKAPAPATPTEEKKPAEQPAKSVPVPEKK"
BASE COUNT          947 a          673 c          659 g         1280 t
ORIGIN      
        1 atccgactat ggccctgata taaacacgcc tgacgtcaag tcggtttttg aaaagattca
       61 ttctaaaatc ctgttcgttc agaagttcat tcagctttgc agtttccact gctctgaaat
      121 tttctcctcc gtcatatatg agctgattca ggctgacccg tatatcctga taaagtgaat
      181 cattctgatt ctggttcaga tttcggatgg aaaaataatt tattcctgcc tttgggagat
      241 attctctcca tttctcccgc attatcagtt ttgtgacttc ttttttccct tcctgaattc
      301 tggaaagaat cctgctggaa cccgaaagag tcagtgtttc ttccagagaa actggaatct
      361 ccctggaatg agtttctgga aatattaaga atatggcaga taatgccgtt ttattaaaaa
      421 tatttataaa tttatttata tttattgaat aaaagaatgc agactgaagc gggattttct
      481 tatttgttcc tggagggaat ccggaggatt ttagtttaca gcacagtatc ctgatgaact
      541 tcggggaatt ccggctgagc tgcttaatgc agcatattga ctgaatgaat ttactgcgga
      601 cagatctgaa aaatccagtt ttcagaggaa cgggttggct gaacggggat gaaatgcatt
      661 tctccatacg tggagattcg tcatatagaa atgtaaaatt aaataaaaag taagcagatt
      721 acatcataga atataacaaa tgatgcaaga tgaactatat cagatcaaaa ccttttcctt
      781 ctctgacaat ctccatttca tcagaagtaa aatcaactat tgtcgaaaat tcgagagtct
      841 tgattccgtc atccacaatt ccgtcgcatc tttttccaaa atgatcttcc agatctttcg
      901 gatcaataat gtattcatca tccctgaata ccgaggtaga gaccagtgca gttccatgaa
      961 tttccagaag agtccgtatg aatatatgat cgcagattct cacaccgatg gatttgttct
     1021 ttgtatggga gtaattgtac ttggaaagtg tcttgcttgc cttgagtatg aatgtaaacg
     1081 gtcctggggt aattcttttc atcagacggt aggctgtatt cggcacatat tcaacataat
     1141 ttgaaacttg cgaaatatca taacaaagaa gggaaagagg cttgtttttg gggatttcct
     1201 tcagaaagta gagtttttcc actcctgtct ttgaaactgg atctgcaatc attgcgtaga
     1261 ctgtatcagt cggaaaaata tagactgcac cagccttcag cttttctgaa atctcccgca
     1321 gtgttctgat ttcagggttt ttgggatgta aatcgatcag cacagactgc tcctgctgtt
     1381 tcaaccaaac agactttctc taataaatcc aatctttgaa atgacttcct gctgtataat
     1441 ataaaattat cagaccgctc ggcaggtgct gcctggcagg aaatttttat tggagataga
     1501 ggtgtttttc actgttttca aatctttttt tatctcgtta tttaaaatac agctgctatt
     1561 tctgagcgcc actcagattt tgatgcataa aatcaggaag catttacaaa taattctgag
     1621 tactctgctg ctgaatcata aaagttcttc attcctgaaa cgaatccgcc agaatccttt
     1681 ttcagtgttc tttctctgat tattttatat gcagttttat aatcattcag aagaataaat
     1741 ccgagtactt tttcaagatg tatatctatt gcagattcag gatatttctt tttatgctta
     1801 aaaagccact catctaaaaa tctgttattt tcttcaatat tttttatttt atctgctaat
     1861 ctgactattt tcttttcatc attattcaat atccttatga tcgcgttttt cagattttca
     1921 ggatttcctt cagaaacaat gactgttttt tcggatatat ccttcatggg tacagtaaat
     1981 gcctgttact cttatactga gagaaagttt ttcattcgat tccattttca ttattttcca
     2041 gaaaactgaa tcaaggtcat aatacttata acctaaactg taaaacattt ttctgtcttt
     2101 attattgaca gtgaataaag cagtaaagac cggatttctt tcctttcgaa aaagaaatcc
     2161 ccctgaagat ttccattttg tctgtttggc aatttcctta atcgatctgt ttaacagttt
     2221 atttacgtct ttctgcagca tagactgtct ttcaatttat cttctaagat aaatcagttc
     2281 tgtttaggga gagtcggatt cggttccggc gaaggtttgt ctgatggaag gttctgaatc
     2341 aaagggtcag ttcctgaatt ggcaggttct ctgaatgttt ctgtcggata acagttgctg
     2401 ttctgctgtc tggatttgtc aaaaagtttt ttataatcca gggagcctga ttcagtcaga
     2461 aattctctgc tgatatagtg ctcaccgttt tcataatagt ttccgaaaga atcgcattcc
     2521 ggaatatctt cattgatgta tttttttttg cagcctgcaa ggattatgca gactgccaga
     2581 aaacagactc tcattttttt tccggaacag gaactgattt tgcaggctgt tcagctggct
     2641 ttttttcttc agtaggagtg gcaggagcag gtgcttttgt cgtactgtcc ttggaggctt
     2701 tagtatcctt ctttggactt tcatcagcag cttttcctaa tctggaattg atcctttttc
     2761 tttcatcctc tctttctttt tctctcagtt tttctttttc ttcataaatc tgatccatac
     2821 agtcgtcgta agttttcaga tgatcctttg gataatagtc ctcatcaatc agaagatttt
     2881 tggaaagtct ttcgttgatc ttcagatttt tgttcgcatt tctttcaata tagtacggga
     2941 ttttcagaag gttgagaatt gcttttttat aaaggtaaag agcttcgata tgattgttct
     3001 tggaggcctg atcaatcgca ttgttctgct gttcattcgc cattgcaatg agtttttcga
     3061 atgtcgaatt gttcagttca tttccggaac ctttgatttt cagctcaact acctttaccg
     3121 aataatctgt gtgaagctga gcataaacag ccttatactt gtctatgaga cttttggcgc
     3181 tttcattcat atcagtgtaa ttctgctcaa atttgcggcg tgccagaaca aaatctcttt
     3241 cactcagtgt tatagtagaa tccacatatc ctttcattgc cgaattgtac ttttcttcca
     3301 tggcattatt gtaaaatgca ctcttcagct ttttcagatg tttcagattg gtatttttca
     3361 acgtgtttgc aatagcctgc ggatcaacag ttacagtgcc tgtttctttt tttgcaggtt
     3421 tagtatcttc ctttttgtct gcagaaggtg aagcatccgt ttttcccgaa actgcaggag
     3481 cagtttcctt tttaggagcc gctgaagcgt ctgtcttagc cggtgcagcc tgtgaagaca
     3541 gtccagaaga aatcagaag
//
LOCUS       DAOQGJ010000153         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14966_length_3558_cov_3.093063, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000153 DAOQGJ010000000
VERSION     DAOQGJ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14966_length_3558_cov_3.093063"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PK683_03485"
     CDS             complement(<1..468)
                     /locus_tag="PK683_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07539.1"
                     /translation="MSFLSKYKIILFSSFLFTVLLMFSFWTASAGFLKTVFEKSNYYF
                     LAVIFIFSLNPIAERYLERYPAYHSFWNENRLKLSLGFLFSVILFFSVPAVFQILADE
                     TNLLSESLSFYKYKTHTNLIEAVEIFGNTSIINQDTAHRPGMYPFLVSLMHSVS"
     gene            complement(673..1425)
                     /locus_tag="PK683_03490"
     CDS             complement(673..1425)
                     /locus_tag="PK683_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNH07540.1"
                     /translation="MNLKLKVWRQKNASDKGKFVDYIEEGKGISTHASFLEMLDIVNE
                     RLESKGEEPIAFDHDCREGICGTCSLFINGFAHGFRKGTTACQLHMHNFKDGDELVIE
                     PWRAKAFPVLRDLIVDRSAFDRIMQAGGFISINTGGAPDANALPIPKTAADLAMDAAA
                     CIGCGACVASCKNASAMLFVSAKVSQLALLPQGQVERKERVRKMLAAMVKEGFGNCTN
                     QYECEAACPKEISVSNIARLNREYLGAVNAEV"
     gene            complement(1436..3346)
                     /locus_tag="PK683_03495"
     CDS             complement(1436..3346)
                     /locus_tag="PK683_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNH07541.1"
                     /translation="MINSKIPSGPLEKKWEKHKFDMKLVNPANKRKYSVIIVGSGLAG
                     GSAAATLAELGYNVSCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVHRLFYDTIKG
                     GDFRAREANVHRLAEISANIIDQCVAQGVPFAREYGGHLSNRSFGGAQVSRTFYAKGQ
                     TGQQLLLGAYSALSRQIGLGTVKMYPRTEMLDVVLVDGHAKGIVVRDMVSGKISSYAA
                     DAVVLATGGYGNVFFLSTNAMGCNVTATFRAYKKGALFGNPCYTQIHPTCIPVAGDYQ
                     SKLTLMSESLRNDGRIWVPKKKGDTRSPFDIPDSERDYYLERKYPSYGNLAPRDISSR
                     SAKEACDDGRGVGPGGLGVYLDFSDAIKRLGEDVIRDRYGNLFDMYSSITNENPYKTP
                     MRIYPAVHYTMGGLWVDYNLMSNIPGLHVIGEANFSDHGANRLGASALMQGLADGYFV
                     LPYTIGDYFANNTVKRPDTDHPEFKKAVAETEEKVKKLFSIDGKRTVDSFHKELGKIM
                     WDECGMARNKAGLEKAIKKIPELREEFWKNLKITGKNEELNQSLEKAGRVADFLEFGE
                     LMCIDALNREESCGGHFRTEYQTEDGEAKRNDDKFCYVSAWEYKGVGKKPVEHKEELV
                     FENIKLAQRSYK"
     gene            complement(3356..>3558)
                     /locus_tag="PK683_03500"
     CDS             complement(3356..>3558)
                     /locus_tag="PK683_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate:quinone oxidoreductase"
                     /protein_id="HNH07542.1"
                     /translation="VLVSYIVSVFLLCFHLSHGLSSLFQTFGANSPKLDPKLKGGALL
                     ISVLIFIGYASVPLAVAAGVIK"
BASE COUNT          946 a          841 c          757 g         1014 t
ORIGIN      
        1 tgaaacagaa tgcatcaagg aaacaaggaa aggatacatc cctggtctgt gtgctgtatc
       61 ctgattaata attgaagtat tcccgaaaat ctcgacagct tcgatcagat ttgtatgagt
      121 cttgtatttg taaaaggaaa gggattctga aaggagattc gtttcatctg ccagaatctg
      181 aaaaactgca ggaactgaaa aaaacagaat gactgagaac agaaatccca aagaaagctt
      241 cagacggttt tcattccaga aggagtggta cgcgggatac ctctccagat acctttcagc
      301 aatcggattc agactgaaaa taaaaatgac tgcaagaaaa tagtaatttg atttttcaaa
      361 gactgtcttc agaaaacctg cagaagccgt ccagaaggag aacatcagca gaactgtaaa
      421 taagaatgag gaaaataaaa tgattttata tttgcttaga aaggacatgg atgtctcctt
      481 gcaggagttt tacagtttat ttaaagctgt cagttccttc cgtaaagaag tttttcagat
      541 tctctccaaa aagttttcca atgtttcaaa agagaactct ctgccaaggg gatgactttc
      601 agattttcaa tgccagatac aaaaaaagcc gcccctggaa aaaaggacgg ctgctttctt
      661 tgagaaaaaa ggttatactt cagcgtttac tgcgccgaga tattctctgt tcagtcttgc
      721 aatattggaa acggagattt ccttcggaca tgctgcttcg cattcatact ggtttgtaca
      781 gtttccgaat ccttccttga ccattgctgc aagcatcttg cggactctct cctttctctc
      841 aacctgtccc tgaggaagaa gtgcgagctg agaaactttt gcagatacaa aaagcattgc
      901 tgaagcattc ttgcaggaag cgacacaggc accgcacccg atgcatgctg ccgcatccat
      961 tgcgagatct gccgcagttt tgggaatagg aagagcattt gcatcaggag caccgccggt
     1021 attgatggaa atgaatcctc ctgcctgcat aatcctgtca aatgcacttc tgtcaacaat
     1081 caggtcgcgg agcacaggaa atgcttttgc tctccatggc tcaattacga gttcatcccc
     1141 atccttgaaa ttgtgcatat ggagctgaca tgcagtcgtg ccttttctga atccgtgagc
     1201 aaatccgttg atgaacagag aacaggttcc gcagattcct tcgcggcagt catggtcaaa
     1261 ggctatcggt tcttcaccct ttgattcaag tctttcattc actatatcga gcatctcgag
     1321 aaaggatgca tgtgtgctga tcccctttcc ttcttctata taatcaacaa atttaccttt
     1381 gtcggaagcg tttttctgac gccatacttt cagtttgaga ttgatttcag ccattttatt
     1441 tgtaactcct ctgagcgagt tttatgtttt caaacacaag ttcttccttg tgttcaacgg
     1501 gttttttccc gactcccttg tactcccaag ccgaaacgta acagaactta tcatcattac
     1561 gcttagcttc tccgtcctca gtctgatatt ctgtacggaa atgtcctccg caggactctt
     1621 ccctgttcag agcatcaatg cacatcagct ctccgaattc caggaaatcg gcaactcttc
     1681 ctgccttttc gagcgactga ttcagttctt cattctttcc tgtaatcttc agattcttcc
     1741 agaattcttc acgcagttcg ggaatcttct tgatggcttt ttcgagaccg gccttgtttc
     1801 ttgccattcc gcattcgtcc cacataatct ttccgagttc cttgtggaag gagtctacag
     1861 tgcgttttcc gtcgatagag aagagttttt tcactttctc ttcagtttct gccacagcct
     1921 tcttgaattc aggatgatca gtatcaggtc ttttgactgt attgtttgca aaatagtcac
     1981 cgattgtgta aggcagcacg aaatatccgt cagcaagtcc ctgcatcagt gcagatgcac
     2041 cgagtctgtt ggcaccatgg tcagaaaagt ttgcctcacc gatcacatgg agacctggaa
     2101 tattgctcat caggttgtaa tccacccaga gtccgcccat tgtgtaatgc actgctggat
     2161 aaatcctcat aggagtcttg tacggatttt catttgtaat gctggaatac atatcaaaga
     2221 ggttcccgta tctgtcgcga atcacatcct caccgagtct tttgattgca tcggagaaat
     2281 ccagatatac accgagtcct ccaggaccga ctcctcttcc gtcatcacag gcttccttcg
     2341 cacttctcga agaaatgtct ctaggtgcca ggtttccgta agaaggatat tttctttcca
     2401 gataataatc tctttccgaa tcaggaatgt cgaaaggaga acgtgtatcg ccttttttct
     2461 tgggaaccca gattcttccg tcgttacgga gagattcgga catcagtgta agtttagact
     2521 gatagtctcc ggctacagga atgcatgtag gatggatctg agtatagcat ggattgccga
     2581 aaagagctcc cttcttgtag gcacggaatg tagcagtgac attgcatccc attgcatttg
     2641 tagaaaggaa gaacacgttt ccgtatccgc ccgtagccag cacaactgca tccgctgcat
     2701 aggaggatat ttttcctgat accatatccc tgacaacgat cccttttgca tgaccgtcca
     2761 cgagcactac atcaagcatc tcagttctcg gatacatttt gactgttcca agtccgatct
     2821 gcctggaaag tgcggaataa gcccccagaa gcagctgctg acctgtctgt ccctttgcgt
     2881 agaaagttct cgaaacctga gctcctccga aggatctgtt ggaaagatgc cctccgtact
     2941 ctcttgcaaa aggaactccc tgtgccacac actgatcgat gatatttgca gaaatttcag
     3001 ccagtctgtg cacattggct tctctggcac ggaagtcgcc gcccttgatc gtgtcataga
     3061 aaaggcggtg cacgctgtct ccgtcattct gatagttctt ggctgcattg atcccgccct
     3121 gggcggcaat actgtgggca cgtctaggtg aatcctggta gcagaaacag gatacattgt
     3181 atcccagctc cgcaagtgtc gcagccgctg aaccgccggc aagacctgag cctacgataa
     3241 ttacactgta ttttctttta ttggcaggat tcacaagctt catgtcaaac ttgtgctttt
     3301 cccatttttt ttccagagga ccggaaggaa ttttagaatt tatcatttct tctccttatt
     3361 taattacacc ggcagcaacg gccagaggaa cagatgcata tccgataaaa atcagtacag
     3421 atatgagcag agcgccgcct ttcagtttcg gatcaagctt tggagagttt gctccaaatg
     3481 tctgaaacag actggacaga ccgtgactca ggtgaaagca gagcagaaat actgagacaa
     3541 tataagaaac aagaactg
//
LOCUS       DAOQGJ010000154         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_14967_length_3558_cov_2.915216, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000154 DAOQGJ010000000
VERSION     DAOQGJ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14967_length_3558_cov_2.915216"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2452
                     /locus_tag="PK683_03505"
     CDS             <1..2452
                     /locus_tag="PK683_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004489248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07543.1"
                     /translation="VDEGSTTKSKIKAVDEGSTTKSKIKAVDEGSTTKSKIKAVDEGS
                     ATKSKIKAVDDSSATKSKIKTVDEGSATKSKIKAVDEGSTTKSKIKTVDEGPITKSKI
                     KAVDEYALIQEASSKQIPVIKMDSSDREPDGKLNTTGRMKAVGSDDWEGEERRKADRT
                     KPKYSLQLKMMAVISLILAVTVSIIISLATYYYKEDNQKRIEEVNLDNVQIVNSKVKT
                     DILSVISQGEQLSVALRKDINKEIGQKLVEQFLKKDEDFIFLGIFGKENGQLVKEKLI
                     FNEEHLKKVSLTEEEILTAVKRKESSFMNSFAGMNVIENTSPGFQEPTLSISIPDAEG
                     RPYILVMMFKLDKILEAFKKKSDGYTVTFMVGADGTILAHSDPAMALSAKNVRDIPIV
                     NEMLTDSKKNNGLTQYDYEGKKYLGAFKKLDFINVGVVTAAELDKAFQEVYRLRNRNI
                     LIMLISLCVSMILVFLFAKSISHPIVDLFSETVKISQGNFQIDIRRTTNDEVGVLTDY
                     FITMAKGLEEREKVKSILGSMIDPIVVQEAMKDLVALRRGSEKTVTAFFSDVAGFSSI
                     SEQLSSADLAALLNEYLSAMTVILKKHGGVLDKYIGDAIVGIFGAPVDLDRHFLKAAK
                     ASVEMIQKLEDLRQYWVRNSLYTKEAQEMDVRIGLNTGLAKVGFMGTNDMGSYTMMGD
                     TVNLAARLEAAGKDYGVNILISEMVNEKIHDEMFTRELDLVRVKGKNEPVRLYELIGR
                     KTEIRQNILEAARIYEEGFKAYLKRDWEKSIKFLNECSKVKGKKDKAALALIERCESY
                     LKNPPAKDWDGVFTRTHK"
     gene            2458..>3558
                     /locus_tag="PK683_03510"
     CDS             2458..>3558
                     /locus_tag="PK683_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH07544.1"
                     /translation="MKGFLQDRSNLTIPVLLALVFVFSYMLCLNIRGAKGQGANPVIG
                     TLTFKKKSIERKFDSDVAWDIVEQGITIHNRDTIRTGDFAEATLTLKDDTKININDNS
                     MIYLDFSDSDINLNFAYGSMSLDKTAGAASENTLKIKTGDKTVEVKNSEVSLEKKDKD
                     EVSLEIKKGDAKVIVGKEEKVIKTNESANIKEKEITVAKISIDLKSPAEGQIFSSRSD
                     TADVGFDWNTQGDLKNARLEIAYDARFNKTVRILNIKSSEASVSLGGGSYYWRITGKN
                     PDTRQTEFSQARKFSIVTIEKPIIFTPSSGQVFSYTSVSPLINIAWKKLENARTYRVE
                     VSRTRDFVQTVRKEVTVQNYVNLDRLEDGVYHI"
BASE COUNT         1201 a          646 c          845 g          866 t
ORIGIN      
        1 agtggatgag ggttccacaa ccaaatcaaa gatcaaggca gtggatgagg gttccacaac
       61 caaatcaaag atcaaggcag tggatgaggg ttccacaacc aaatcaaaga tcaaggcagt
      121 ggatgagggt tccgcgacca aatcaaagat aaaggcagtg gatgacagct ccgcgaccaa
      181 gtcaaagatc aagacagtgg atgaaggttc cgcgaccaag tcgaagatca aggcagtgga
      241 tgagggttcc acaactaagt caaagatcaa gacagtggat gaaggtccca taaccaaatc
      301 aaagatcaag gcagtcgatg aatatgcttt gattcaagag gcatcatcga agcagattcc
      361 cgtaatcaag atggattcgt ctgacagaga gccggacgga aaactgaaca ccacagggag
      421 aatgaaagcc gtaggttctg acgactggga aggagaggaa agaagaaaag ccgacagaac
      481 aaaaccaaag tattctcttc agctgaaaat gatggcagtg atttctctaa tcctggctgt
      541 tacagtttcc attatcattt ccctcgctac atattattat aaagaagaca atcagaaaag
      601 aattgaagaa gtcaacctgg acaatgttca gatcgtaaac tcaaaagtga agacagatat
      661 cctttctgta atcagtcagg gagagcagct ttctgttgca ctgagaaagg acataaacaa
      721 ggaaatcggt cagaagcttg tggagcagtt tctgaaaaag gatgaggatt ttattttcct
      781 gggtatcttt ggaaaagaga acggacagct tgtcaaagag aaattaattt ttaatgaaga
      841 acatctgaaa aaagtttctt tgactgagga ggaaattctt actgcagtca aacggaaaga
      901 atcttctttc atgaattctt ttgcgggaat gaatgtaata gaaaatacta gtccgggatt
      961 tcaggaacct acactctcta tcagtattcc tgatgctgag ggaagaccct atatacttgt
     1021 gatgatgttc aagctggaca agattctgga agcattcaag aagaaaagcg acggatacac
     1081 tgttactttc atggtcgggg ctgacggcac gatactggca cattctgatc cggctatggc
     1141 tctttccgca aagaatgtac gggatattcc cattgtaaat gaaatgctca ctgattccaa
     1201 gaaaaacaac ggactcactc agtatgatta cgaggggaaa aaatatctcg gtgcattcaa
     1261 aaagctcgat ttcatcaatg taggtgttgt gaccgcggct gagctggaca aggcttttca
     1321 ggaagtgtac agactcagaa acaggaatat cctgatcatg ctgatatcat tatgcgtttc
     1381 catgattctg gtattccttt ttgcaaagtc catctctcat cctatcgtag acttgttttc
     1441 ggagacagtt aaaatctccc agggaaattt ccagatcgac atcagaagaa ccacgaatga
     1501 tgaagtaggg gtactgactg attatttcat tacgatggca aaaggtctgg aagaaaggga
     1561 aaaggtcaag agcattctgg gaagtatgat tgatccgatc gtggttcagg aggcaatgaa
     1621 agatcttgtc gcccttcgac gaggcagtga aaagacagtt actgcattct tttcagacgt
     1681 tgcaggattt tcctcgatca gcgagcagtt aagctcagca gatctcgcag ctcttctgaa
     1741 tgagtatctt tccgcaatga ccgtgatttt gaaaaaacac ggaggtgttc tggataaata
     1801 tatcggggat gcaattgtag gaatattcgg tgcacctgtc gacctggaca gacactttct
     1861 taaagctgct aaagcttctg tggaaatgat tcagaaactg gaagatctga gacaatactg
     1921 ggtgagaaac agtctgtata ccaaagaagc tcaggaaatg gatgtaagga tcggtctgaa
     1981 cactggactt gccaaagtag ggtttatggg aacaaatgat atgggttcct atactatgat
     2041 gggggatact gtcaatcttg ctgcgcgtct cgaagcagcc ggaaaggatt acggagtcaa
     2101 tattcttatt tctgaaatgg tgaatgaaaa gatccatgat gagatgttta ccagggagct
     2161 tgacctcgtt cgcgtgaaag gtaaaaatga acccgtcaga ctttatgaac tgataggccg
     2221 aaaaactgaa atcaggcaga atattctgga agcagccaga atttacgagg aaggctttaa
     2281 ggcttacctg aaaagagact gggaaaaatc cataaaattt ctgaacgagt gcagcaaggt
     2341 aaagggaaaa aaagacaaag ccgccttggc actgattgaa agatgcgaaa gctatttgaa
     2401 aaatccaccg gcaaaagact gggatggagt attcacgaga actcataaat aacagtgatg
     2461 aaaggatttt tacaggacag atcgaatctt accatcccgg tactgcttgc gcttgtcttt
     2521 gtgttttcgt atatgctctg cctgaacatc agaggcgcaa aggggcaggg agccaatcca
     2581 gtaataggaa cgctgacatt caaaaagaaa tctatagaaa gaaaatttga ttcggatgtg
     2641 gcctgggaca tcgttgaaca gggaatcaca attcacaatc gggatacaat tcggacagga
     2701 gactttgcgg aggcaactct gaccctgaaa gacgatacaa agataaatat taatgataac
     2761 agcatgattt atctggattt ctccgacagt gacatcaatc tgaattttgc ctatggatct
     2821 atgtcccttg acaagactgc cggagctgca tcagaaaata cactgaagat caagacagga
     2881 gataaaactg tagaagtaaa gaattctgaa gtatcgcttg agaaaaagga caaggatgag
     2941 gtttcactgg aaattaaaaa aggtgatgcg aaggttattg tcggcaaaga agaaaaagtc
     3001 ataaaaacta atgaatctgc aaatataaaa gaaaaagaaa ttactgtagc aaaaatatcc
     3061 atcgatctta aatctcctgc ggaaggccag atattcagtt ccagatcaga tactgcggat
     3121 gttggattcg attggaacac tcagggtgat ctgaagaatg cccgacttga gatagcatac
     3181 gatgcaagat tcaataaaac agtcaggata ttgaatataa aatcatccga agcaagtgtc
     3241 agtcttggcg gaggaagcta ttactggagg ataacaggaa aaaaccccga caccaggcag
     3301 actgaatttt ctcaggccag aaaattttcc atagtcacta ttgagaagcc gattattttt
     3361 actccttcca gcggtcaggt tttcagttat acatctgttt ctcctttaat taatattgca
     3421 tggaaaaaac tcgaaaatgc aagaacatac cgtgtggaag tcagcagaac cagggacttt
     3481 gttcagactg taagaaagga agtcactgtt cagaactatg tcaatctgga cagacttgaa
     3541 gacggagttt atcatatc
//
LOCUS       DAOQGJ010000155         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15048_length_3544_cov_2.521926, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000155 DAOQGJ010000000
VERSION     DAOQGJ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15048_length_3544_cov_2.521926"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PK683_03515"
     CDS             <1..589
                     /locus_tag="PK683_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07545.1"
                     /translation="LSNGQVTTLITDNTNLKNPRGMVVYGNYLYISEKNNGKILELNL
                     STNVLNPVASGLNDPRDMTLVGSTLYISDKSADAVYSVGIGIWTLNTLAGGVQGHLNG
                     IGTAAKFKNPEGIVSDGTNLYICDTGNNSVRKIDLFSGTVSTVAGNTPPSTTSGYVNS
                     SLSSSARFNSPRGITSDGVSLYVSDRNNNTVRKIN"
     gene            591..2039
                     /locus_tag="PK683_03520"
     CDS             591..2039
                     /locus_tag="PK683_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07546.1"
                     /translation="MSDGIKIIIFPVEKNLDIPPNPYPRVDIGGFLSMERKIYIKTVS
                     LLFILIFSSGCLARKVKLKYGNTSILLGTFALGGAVNNLFGTLVLKNSNGDTVTVKSS
                     GQFVFSLKLADAVSYSVSVSSQPSMQNCILENPEGVINSADVTNIKVTCTGNSYGPLV
                     SGTIVAPLSFTNGVTTLAGSYAAPNSAGTSNSGFTNSADASAVQFSGPEGIATDGTNL
                     FIGDKNNNVIRKTVIATGETTTLAGSGVRGILDGTGTSAKLDTPVDLTCDGVYVYFSD
                     PSNRAVRKAEISTGKVTTIISNNSKLVTPKGLIVYNNKLYISEDTNNAIMELDLYTLS
                     VKTVLTSGISNPQSMTLVGSTMYISDRNTHTIRKTVIGTWTGSIFAGISGNSGHIDGT
                     ATSARFNSPEGITNDGNYLYVTESNNCIRQIDISTGTVTTLAGPSAGNSSGYTNSSVH
                     LNALFSSPRAITSDGSALYLADRTNSAIRKIN"
     gene            2104..2571
                     /gene="bfr"
                     /locus_tag="PK683_03525"
     CDS             2104..2571
                     /gene="bfr"
                     /locus_tag="PK683_03525"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017850508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNH07547.1"
                     /translation="MKGNSEIIEILGEVLSAELTAINQYFIHSKMNQNSGYKKLADYM
                     KKESIEEMKHADMVIERILFLDGVPDLQRYMKIKVGKNITEMLQADLEVEYAAVERLN
                     RGIDLCVKFKDNGSRELLEKILVSEEEHIDWIEAQQQIIADIGAANYLAQQLE"
     gene            2927..3307
                     /locus_tag="PK683_03530"
     CDS             2927..3307
                     /locus_tag="PK683_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNH07548.1"
                     /translation="MKIRDRRKEITIYIKNDEIVDGKLFHKTLLDKLVELHVTGCTIV
                     KSNAGFGMDMKLKYTDSYFTEFWSRESTVIITVIETESKTEEIIALLDSCMPQGIVSI
                     KDVEVIRYTKTVVTDEDIKLAENA"
     gene            3308..>3544
                     /locus_tag="PK683_03535"
     CDS             3308..>3544
                     /locus_tag="PK683_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH07549.1"
                     /translation="MKHEISKIRNFSIIAHIDHGKSTLADRLLELTLVTDQRTKKDQI
                     LDTMDIERERGITIKANNASFDFKSKDGNTYLFNL"
BASE COUNT         1140 a          676 c          781 g          947 t
ORIGIN      
        1 tctgtcaaac ggtcaggtga ctacactgat tacagacaat acaaatctga agaatcccag
       61 aggaatggtt gtatacggaa attatcttta catttcagaa aaaaataatg gaaagatttt
      121 agagctgaat ctgtctacca atgttctgaa tccagtcgca tcaggtctga atgatcctag
      181 agatatgact ttggtcggtt ccactcttta catatccgac aaaagtgcag atgcggtgta
      241 ttccgtggga atcggtattt ggacattgaa cactcttgct ggcggcgttc agggacatct
      301 taacggaatc ggtaccgcag caaaatttaa aaatccggaa ggaattgtct ctgacggtac
      361 aaacctttat atctgcgata ccggcaataa ttctgtcaga aaaattgatc ttttttccgg
      421 aactgtaagc acggtggcag gaaatacgcc gccttccaca acatcaggat atgtcaattc
      481 atccctgtct tcgagtgcca ggttcaactc cccgcgagga attacgagcg acggagtcag
      541 tctgtacgtc tctgacagga acaacaatac agtcaggaag ataaactgaa ttgtccgatg
      601 ggataaaaat catcatattc cccgttgaaa aaaatcttga catcccgcca aatccctatc
      661 ccagagtgga tattggggga tttttatcca tggaaaggaa aatatatata aaaactgtct
      721 ccttattatt catacttatt ttttcatcag gatgtctggc aagaaaggtt aagctcaaat
      781 acggcaatac atctattctg ctcggaactt ttgccttggg aggagctgta aataatttat
      841 ttggaaccct ggtgctgaaa aacagcaacg gggatacagt tacagtcaaa tccagcggac
      901 agtttgtgtt ctctctcaaa cttgcagatg cagtatccta ttcagtcagt gtaagttctc
      961 agccctctat gcagaactgt attttggaaa atccggaggg ggtgatcaat tctgcggatg
     1021 tgacaaatat aaaagtgacc tgtacaggaa attcttacgg tcccttggtg agcggaacta
     1081 ttgtcgctcc tctgagtttt acaaatggag tcactacttt ggccggttcc tatgctgcac
     1141 cgaattctgc cggtacttca aattcaggct ttactaattc ggcagatgca agtgctgttc
     1201 agttcagcgg ccctgaagga attgccactg acggaacaaa cctctttata ggagacaaaa
     1261 ataacaatgt cattcggaaa acagtgattg cgacgggaga aaccacgaca ctcgcaggga
     1321 gcggggtcag aggaattctc gacggaacag gaacctctgc taaactggat accccggtgg
     1381 atctgacctg tgacggagta tacgtatatt tttctgatcc cagtaacagg gctgtaagga
     1441 aagcggaaat ttctacaggg aaagtaacta ctatcatctc aaacaacagc aaacttgtga
     1501 ctccgaaagg tttgatcgtt tataataata agctttatat ttctgaggat acaaataatg
     1561 ctattatgga actggatctg tatacattga gcgtaaaaac tgttctgact tcgggaattt
     1621 caaatccgca gagtatgact cttgtcggaa gcacaatgta catcagtgac cgaaatacac
     1681 acacaataag gaagacagta atcggtacat ggacaggatc aatatttgcc ggtatttcag
     1741 gaaattcggg acatattgac ggaacggcca catctgctcg gttcaacagt ccggagggaa
     1801 ttacaaatga cggaaattat ctttatgtca ctgaatcaaa caactgcatc cggcaaattg
     1861 atatttccac gggtactgtc actacacttg caggaccttc ggcaggaaac agcagcggtt
     1921 atacaaattc gtctgtacac ttgaacgcgc ttttcagttc acctcgtgcc ataacaagcg
     1981 atgggagtgc tttgtatctg gcagacagaa ccaattctgc aatacgcaaa atcaattgat
     2041 ttaatttcga cctgaaaata atcagaaaat gaacaatgga aagagaaata cgaggagatg
     2101 gttatgaaag gaaattctga gattattgaa attttagggg aagtcttaag tgctgaactg
     2161 acagcaatca atcagtattt tattcattca aaaatgaatc agaacagcgg ttacaaaaaa
     2221 ctggctgatt atatgaaaaa agagtctatc gaagagatga aacatgcaga tatggtcatc
     2281 gaaaggattc tttttctcga tggagtgcct gatctgcaga gatatatgaa aatcaaggtc
     2341 ggtaaaaata ttactgaaat gcttcaggcc gatctggaag tggagtatgc tgctgtggaa
     2401 agactcaatc ggggcattga tctctgtgta aagtttaagg acaacggatc gagagagctc
     2461 cttgaaaaaa ttctagtctc tgaagaagaa catattgact ggattgaagc acagcagcag
     2521 atcatcgctg atatcggtgc ggcaaattat ctggctcagc agctggaata gatcaaagac
     2581 gcagtttcct tggacgtaaa tgctgtgtaa gtgaactgtc agcagtctca gatttttcct
     2641 tactcggtat ttcgttcagg catttgcaaa gaacaattcc ttcattgtaa aagcagaact
     2701 tttttgcaga taaaagacgg aaataatcgg attactccct cttttagagg tccgaaagaa
     2761 tatctcttcc ataaagcaag cagtttttgt ctcaaatata cgatgaattc atatgattcc
     2821 aatgaatttt tccttgtaaa ttcctaacgc tttttttgta ctcaattcag atgagacgga
     2881 gaagtgaggc agtacaaagc tgttccatta tccgaaacga gggaaaatga agatcagaga
     2941 cagaagaaaa gaaatcacca tttatataaa gaacgatgag attgtggacg gaaaactttt
     3001 ccataaaact cttctggata aactggtgga gcttcatgtg acaggctgca cgatcgtgaa
     3061 gtccaatgcc ggtttcggaa tggatatgaa gctgaaatac actgacagct attttactga
     3121 attttggtcg agggaatcca ctgtcattat tactgtcata gaaactgaat cgaagacgga
     3181 ggagattatt gcccttctgg attcctgtat gccgcaggga attgtgagta taaaagatgt
     3241 ggaagtaata cgttatacga aaactgtggt tactgacgaa gatattaaac tggcggaaaa
     3301 tgcctaaatg aaacacgaaa tcagtaaaat ccgaaatttc tcgattattg cacatattga
     3361 tcatgggaaa tccacacttg cagacaggct gctggaactt accttggtga cagatcaaag
     3421 aaccaaaaaa gaccagatcc tggatacgat ggatattgaa cgagaaagag ggattacaat
     3481 taaggctaac aacgcatcct tcgatttcaa atccaaggat ggaaatacgt atctgttcaa
     3541 cctg
//
LOCUS       DAOQGJ010000156         3543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15052_length_3543_cov_3.628727, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000156 DAOQGJ010000000
VERSION     DAOQGJ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15052_length_3543_cov_3.628727"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PK683_03540"
     CDS             complement(<1..918)
                     /locus_tag="PK683_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH07550.1"
                     /translation="MTKCIFINLLADMNIPEEHIENGEKLLKNGNFEGAVSSFSKAID
                     LVPDNPYYYYKRGRAYEESKDYEKALKDFVKGSSLDSKSALYVSAAAGVYEAQENYDK
                     ALEFFQKSCSLDSRSAWDHYKVGDIHFINKKYSEAVSWFTKAIELKEDEYYCYRRGKA
                     YLELGDRESAKRDFSRAVQISPDYKLGLMERAPLLFEEGDYEGCISDYTALIEKYGGQ
                     AYLYYFRGKAYFELQKYAEAAADFSESIKLDPSDINCFLKRGDAYLYIKEYEKAKADY
                     TVSIEADPENFPSYFARGNSHYALNELDDA"
     gene            complement(949..1338)
                     /locus_tag="PK683_03545"
     CDS             complement(949..1338)
                     /locus_tag="PK683_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004504149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNH07551.1"
                     /translation="MVLADTSVWIEFFKRKEPVFSELRELIEASEVKVHEVIFGELLQ
                     GCKNRSEERMIIDYWENLGEPESDASFIEAGRLSYSEKHLSKGVGLIDSVIISQARKL
                     DCRIWTLDKKINNILHKSEIYRPKNTA"
     gene            complement(1338..1553)
                     /locus_tag="PK683_03550"
     CDS             complement(1338..1553)
                     /locus_tag="PK683_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HNH07552.1"
                     /translation="MKVTAILPDELISDVRELTGGKNITESIEKALTEWIRLAKLKRL
                     NARVSEKPLAFSEEFSAEKARKTNRIR"
     gene            complement(2231..2788)
                     /locus_tag="PK683_03555"
     CDS             complement(2231..2788)
                     /locus_tag="PK683_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07553.1"
                     /translation="MILPENIVSKFNSLNGKNNFEKMIGTVGILTHLLDEKQRPVIVG
                     GLAVEIYTRNEYTTVDIDLILNERARADEILSGLGFQKQGRHWFHPVLLVSLEIPNFV
                     LEDADSEKVLELILPDEMKVYVIGLEDIILDRLRACVHWKSESDCEWAERMYFLHSEK
                     LDMEYMRRTAKKDRTLEHFEKMIFS"
     gene            complement(2775..2942)
                     /locus_tag="PK683_03560"
     CDS             complement(2775..2942)
                     /locus_tag="PK683_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07554.1"
                     /translation="MKILRAGRKRTYRRRPRDPEEIAVLNQICLLRWKKAEAEGKIRY
                     ISKTQWYYDPS"
     gene            complement(3348..>3543)
                     /locus_tag="PK683_03565"
     CDS             complement(3348..>3543)
                     /locus_tag="PK683_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07555.1"
                     /translation="AGSDGRDDLDRTFGEDRTISYSAETFLICVEKAKGQILEIEKEI
                     LKIGNLTDDFSLIRIEIKFR"
BASE COUNT          972 a          792 c          613 g         1166 t
ORIGIN      
        1 tgcatcatcg agttcattca gagcatagtg actgtttcct cttgcaaaat acgatgggaa
       61 attttccgga tcagcctcaa tggatacagt ataatccgct tttgctttct cgtattcttt
      121 aatatacaga tacgcatctc ctcttttcag aaaacagttt atgtcggaag ggtcaagctt
      181 tatggattca ctgaagtccg ctgcagcttc tgcatatttc tgcagttcga agtatgcttt
      241 tcccctgaaa tagtaaagat aagcctgtcc accgtatttt tcaatcagag ctgtatagtc
      301 agaaatacat ccttcgtaat ccccttcttc gaaaagcaga ggtgcccttt ccatcagtcc
      361 aagtttatag tcaggactga tctgaacggc tctgctgaaa tccctttttg cactttctct
      421 gtcaccgagt tcaaggtaag ccttgcctcg tctgtaacag tagtattcat cttctttcag
      481 ctctattgcc ttggtgaacc aggaaactgc ctcagaatat tttttattta taaaatggat
      541 atcaccgact ttgtaatgat cccaggcact tcgggaatca agactgcagg atttctgaaa
      601 gaactcaaga gccttgtcat aattttcctg agcctcatag actccggctg cagcagaaac
      661 atacaaggca gacttggaat caagtgaaga gcctttaacg aaatctttca gagccttttc
      721 atagtccttg ctttcttcat atgccctgcc tcttttatag taataataag gattgtcagg
      781 cacaaggtct atcgctttgg aaaatgagga aacggcacct tcaaaatttc cgtttttaag
      841 aagtttctca ccgttttcaa tatgctcttc aggtatattc atatctgcaa gcagattgat
      901 gaatatgcat ttggtcaatg ataaaatgtt tcgggataat cagaagaatc aggctgtatt
      961 tttcggacga taaatttctg atttatgcaa tatattattt attttcttat caagagtcca
     1021 aattctgcag tcaagtttcc ttgcctgact gatgattaca gaatctatca gaccgactcc
     1081 cttgcttaaa tgtttctcgg aataagaaag gcggcctgct tcaataaatg aagcatctga
     1141 ttccggctcc cccaggtttt cccagtaatc aatgatcatc ctttcttctg acctgttttt
     1201 acatccttgc aggagttctc caaatattac ttcgtgcact ttaacttccg aagcctcaat
     1261 caattcccgc agttcactga atacaggctc tttacgtttg aaaaattcaa tccatacaga
     1321 tgtatctgca agcaccatca gcgtatcctg tttgtttttc tggctttctc tgctgaaaat
     1381 tcctctgaaa aagctagggg cttttcagat actcttgcat tcagcctttt cagtttggcc
     1441 agcctgatcc actcagtaag tgctttttca attgattcag taatgttttt tccgcctgtc
     1501 agttctctga catcggaaat cagctcatca ggcagtattg cagtaacttt cataagatac
     1561 aagatacgat acactatcgt atagtcaata tttttttatg agaagaagga tcaattgaac
     1621 acttttccat aatattttcg ggagagactc ccgcaaattc tcataatatc tgctcgatac
     1681 gatgaaagcc gaaaaatgtg ctcacattca cttatatgtt tttccgccct gttttcctgt
     1741 tcattccttt ccttccagtt tctgcatttt gactggatgt attctgctct tctaaccaga
     1801 agcttactgg cttctagcaa attatcaaac atcggatctg ggtcagtttc ttctccgtta
     1861 cagctgattg tctgataaag agaacttaat tttggtttac tttgattctc agcacagata
     1921 caaaaatctc cgcatacatc ggagcacaga agtgttccaa aaagcattga tctgcctagc
     1981 cttctttaaa gacccagcct cacttttctt cagtactgaa ttttgtaatg catacttatt
     2041 gtatgaaaag aaagtatctt ttgtattggc cagccaggca gattctctct tgtaggattt
     2101 gacgagaggc atcaatgctt tttcttcagt ttttgtattt tcatgaagac aagaaggaag
     2161 cagccaaaat aagcaaatac ataatgatat gtaaaatatt atacgttgaa tcatatcaca
     2221 aataatttta tcatgaaaat atcattttct caaaatgttc cagggttctg tcttttttgg
     2281 cagttcttct catatattcc atatccagtt tttcactgtg aagaaaatac atccgctctg
     2341 cccattcaca gtcagattca gatttccagt gaacacaggc acggagccgg tctaaaatga
     2401 tgtcttccag cccaatgaca tacactttca tttcatccgg aagaatcagc tctaaaactt
     2461 tttctgaatc agcatcctcc agaacaaaat tcggaatctc caggcttaca agaagaactg
     2521 ggtgaaacca gtgtctgccc tgtttctgaa acccaagtcc ggaaagtatt tcatcagctc
     2581 ttgctctctc attcagaatc agatcaatat ctacggtggt atactcattt ctggtataaa
     2641 tctccacagc aagaccgcct acaattacag gtctctgttt ttcatcaaga agatgtgtca
     2701 gaatacccac agtgccgatc atcttctcaa aattgttttt cccgttcaat gaattgaact
     2761 tactgacgat gttttcagga aggatcatag taccactgcg tttttgagat ataacgtatc
     2821 ttgccttctg cttccgcttt tttccagcgc agcagacaaa tctgattcag aacagcaatc
     2881 tcttccggat ccctcggtct gcggcgatag gtacgctttc ttcctgcccg cagaattttc
     2941 atttccgttt ctgacttgcg gctttcaata aaacgattca gatcttccag tctccagact
     3001 tgcacagtta cagaaataca cactctctca gttctgcaat cagaaaatca tactgtctca
     3061 ctttacgtct ctgtctgtag acgggcattt ttgtccggct cgaagaaaag gaaactgcct
     3121 tcctgctcaa tgcaggaaag cgggctttca gtattttaga agctgctgag gtctgctttt
     3181 atcgattcat cacaagccgt caacctcctg gatttttcaa tggttcgtaa tatttgcata
     3241 ttccatacag aagattcttt ttccgtaaga tgagtaatca aagcagcagt gcttctgctg
     3301 aattctgtca aagaatataa taaatttaat aatatataat tataatatca tctgaattta
     3361 atttcaatcc tgatcagtga aaaatcatca gtcaggttcc ctattttgag aatttctttt
     3421 tcaatttcca gaatctggcc ctttgccttt tccacgcaga ttagaaaagt ttctgctgaa
     3481 tagctgattg ttctgtcttc accgaaggtt ctgtccagat catcccgacc gtcggaaccg
     3541 gca
//
LOCUS       DAOQGJ010000157         3543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15053_length_3543_cov_3.305046, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000157 DAOQGJ010000000
VERSION     DAOQGJ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15053_length_3543_cov_3.305046"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..859)
                     /locus_tag="PK683_03570"
     CDS             complement(20..859)
                     /locus_tag="PK683_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNH07556.1"
                     /translation="MKKIYFLSFFVSFLLYSEEIPDFRMRRVEAGQYYKMRIGTVKEP
                     EKRMVFYKVVISKPYKLSATEVTVGQWKAVMGGESPYQFSKGDDYPVTNVSYNDIQEF
                     LKKLNRNSSGKPYRLPTYAEWELAAKGPANSEKEQKIFYWGNNPALGNDYEWNIDNYG
                     NESHKVGTRKPNPNGIYDLWGNAGEWLSDGRINASTRELRLGNKGDGICLLENDPDYC
                     YPADHTVTDPAGDAGADRFCIIEFCEASILKEVGTTDEYYKAFRREYKTGRLGFRLAQ
                     DAD"
     gene            complement(859..2286)
                     /locus_tag="PK683_03575"
     CDS             complement(859..2286)
                     /locus_tag="PK683_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH07557.1"
                     /translation="MYFREFSAYFFFYILTLQISAQQYNLSKVSGKETEKHFYGQFKE
                     LKTRSGQTKIVYESLKNSRSMEPDRDEKIAYAVYSGNFRAVQKLLSEGADINARMYPG
                     CGPKGHSIFLALTSGSAAMTRLVIEKGGDVNYTLPAGMHGIPYGWYLAGLQEIADEMG
                     RRDLKDILKSKKGLKGHIRNVGNLHEYFAFKPYSPDSSLKPVSGRPNRNDFTGQYDYP
                     AVCRYTDGEFSYGLLKKNQETGTMVCDQRSYKSDKIQFRDSPFEVLYHLKPLKKNQEI
                     VWEVPLPWEGEKGIEETYSHSFQPPDDNKVLKFNAVFEYYTVYMQETVFSKPNSESKE
                     SYIKNKYYHFMLTLVEFDSDSSEADNIPSPELNLQLLKAAFSGNSEAVKSALKSGADP
                     NAQNKGWTSLMYAAYYGKTDVIRILLEYSADTNIKYQGYTALNIAEKKGNTEAAELLK
                     PASRSLRPTVLPRSLVPEPSAPGVK"
     gene            complement(2466..2768)
                     /locus_tag="PK683_03580"
     CDS             complement(2466..2768)
                     /locus_tag="PK683_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07558.1"
                     /translation="MKKIIYLLIIFLFIFPAQYVQAQSLEVKIESKYSIDGRSVTEAE
                     FNQFKDTLKEVKGTWFCAETNTGGITGYNAADKDGTVYEIRFISDSQKDENSIRKK"
     gene            complement(3104..>3543)
                     /locus_tag="PK683_03585"
     CDS             complement(3104..>3543)
                     /locus_tag="PK683_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07559.1"
                     /translation="GALYSGGAALLLLTLYETRKQALAAKTENHRDAAANFMLAAVPS
                     TISSDLRIAYSVSANAKTFTADQHKVDQYNGALFALAVFYGVQMVHIIYNGFAWENGL
                     LIVDSQKKEPGRINTYLTAMPESLPNTERKSGVAFHGGLTVNF"
BASE COUNT          967 a          786 c          694 g         1096 t
ORIGIN      
        1 tatttgtcaa tgtaaaagtt cagtcggcat cctgtgccag ccggaagccg agcctgccag
       61 tcttgtattc tcttctgaat gctttgtaat attcatctgt ggttccgact tcttttaata
      121 tggaagcctc acagaattca atgatgcaga atctgtctgc tcctgcatct cctgcgggat
      181 ctgtaacagt atggtcagcc ggataacagt aatcaggatc attttccaga agacatattc
      241 cgtcaccttt gtttccaagt cgcagttctc tggtagaagc attgatcctt ccgtcagaaa
      301 gccattctcc ggcatttccc caaagatcat aaattccatt aggatttggc ttgcgtgttc
      361 cgactttatg cgattcattt ccgtaattgt caatattcca ttcataatca tttcccaaag
      421 cgggattatt tccccaataa aatatcttct gttctttttc agaatttgca ggtcccttgg
      481 cagcaagctc ccattctgca tatgtcggaa gccgataggg ttttccggaa gaatttctgt
      541 tcagcttttt cagaaattcc tgaatgtcat tataagaaac atttgtgaca ggataatcat
      601 ccccttttga aaattgataa ggtgactcac ctcccattac agccttccat tgtcctacag
      661 ttacttcggt tgccgacagt ttgtaaggtt tagatattac aaccttataa aataccatcc
      721 gtttttcagg ctctttgact gtcccgatcc tcattttata atactggcct gcctctacac
      781 ggcgcatacg gaaatcaggg atctcctcag aataaagaag aaaggaaaca aaaaaagata
      841 aaaaatatat ttttttcatt attttactcc gggtgctgac ggttcaggaa caagagaccg
      901 gggaaggaca gtcggtctca gagaacggct ggccggcttc aaaagttcag cagcttccgt
      961 atttcctttt ttttcagcta tattaagtgc agtatatccc tgatatttta tattcgtatc
     1021 ggcactgtat tcgagaagaa tacgaatgac atccgtcttg ccgtaataag ccgcatacat
     1081 cagactggtc catcctttat tctgagcatt aggatcagcc ccggatttca gtgcagattt
     1141 tactgcttca gaatttccgc tgaaggctgc cttcagaagc tgaagattca gttccggtga
     1201 aggaatattg tccgcctcac tgctgtctga atcaaattcc accaaagtca gcataaaatg
     1261 ataatactta tttttaatgt aactttcttt actttcagaa ttcggtttag aaaagactgt
     1321 ctcctgcata taaacagtat agtattcaaa caccgcattg aatttcagca ctttattatc
     1381 atccggcggc tgaaatgaat ggctgtaagt ttcctcaatg cctttctcgc cttcccaggg
     1441 aagcggaact tcccagacaa tttcctgatt tttcttcaaa ggtttcagat ggtagagaac
     1501 ttcaaatgga ctgtctctga attgaatttt gtctgattta taagatctct ggtcgcagac
     1561 cattgttccg gtttcctgat tttttttcaa caatccgtat gaaaattcac cgtccgtata
     1621 tctgcagaca gcagggtaat catactgtcc agtgaaatca tttctgttag gtctgcctga
     1681 aaccggcttc aatgaggagt cgggggagta cggtttgaaa gcaaaatatt catgcagatt
     1741 gcccacgttg cggatatgtc ccttcaaccc tttttttgat ttcagaatat ctttcaggtc
     1801 acgtctgccc atttcatcag caatttcctg aagaccggca agataccatc cgtaaggaat
     1861 gccgtgcatt cctgcgggca gagtataatt gacatcgcct cccttttcaa tgactagtct
     1921 ggtcattgca gcagaaccgg aggtaagtgc aagaaatatg gaatgacctt ttgggccgca
     1981 gccagggtac attcttgcat tgatatccgc tccttcagaa aggagttttt gaactgccct
     2041 gaaattgccg ctgtaaacag cataagcaat cttttcatct ctgtcgggtt ccatacttcg
     2101 ggaatttttc aggctttcat aaacaatctt agtctgacct gaacgggtct tcagttcctt
     2161 gaactgtccg taaaaatgtt tttctgtttc cttcccggac actttagaca gattgtactg
     2221 ctgtgcagaa atctgcaagg tcaatatata aaagaagaaa tatgccgaaa attctctgaa
     2281 atacatataa tctccgtaaa tcagcaaatt ggtttctcca acaagaactc aatttgccga
     2341 aaaatatgtc agactgatat ttcgaataat ttcctacagt tcagaactgt caatttttta
     2401 tttcggcaga ggacgctttt attataagca taatcgtcca gctctgcaac tgtgcatcca
     2461 ctgacctatt tttttcggat ggaattctca tctttctggc tgtcggagat gaatctgatt
     2521 tcatagactg ttccgtcttt atctgcagcg ttgtacccag tgatgccgcc agtatttgtt
     2581 tcggcacaga accatgttcc tttgacttcc ttcagtgtat ccttgaactg attgaactct
     2641 gcctctgtga cagatctccc gtctatggaa tactttgatt caatcttgac ttccaggctt
     2701 tgtgcctgca catattgtgc aggaaagata aataaaaata ttattaataa atatataatt
     2761 tttttcatag aaactcctga tatgcggcat agcccgaaaa aaccgttctg acgcatactg
     2821 ccgacaagtc aaactgtttt gcatttccgg caggacattt taaactatcg gcacatatat
     2881 tcagtgtatg caggaatgaa ccgccatttc agaaagagct gatcaattac tgtttacgga
     2941 ttaatattga gatgctccgt tgagcaaaaa ttgctgtgtc tagacacatc atatttgcgc
     3001 aaggcgagac agtaaaataa acataaataa aaaaattata agtttttata aactttcatt
     3061 ctgccgccgc accgtcagac agcagatatt tcaggataga ccgtcagaaa ttcactgtca
     3121 gtccgccatg aaaagcgact cctgatttcc tttctgtatt tggaagagat tcaggcatag
     3181 cagtcagata ggtattgatt ctgccaggtt cttttttctg actgtccaca atcaaaagac
     3241 cgttttccca ggcaaatcca ttataaatga tatgaaccat ctgaaccccg tagaatacag
     3301 ccagcgcaaa cagtgctccg ttgtactgat ccaccttgtg ctgatctgca gtaaaggttt
     3361 tggcgttggc agaaacactg taggcgatgc ggagatcgga gctgattgtg ctgggaactg
     3421 cggcaagcat aaaattggcc gcagcatccc gatgattttc cgtttttgca gccagtgcct
     3481 gcttgcgagt ttcatacaga gtcaacagaa gcagggctgc tccgccggaa tacaatgcac
     3541 cga
//
LOCUS       DAOQGJ010000158         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15140_length_3528_cov_3.128707, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000158 DAOQGJ010000000
VERSION     DAOQGJ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15140_length_3528_cov_3.128707"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..749)
                     /locus_tag="PK683_03590"
     CDS             complement(303..749)
                     /locus_tag="PK683_03590"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH07560.1"
                     /translation="MKYSVEIEINLPIARVIELFDNPDNLQKWQPGLLSFEHLSGTPG
                     QPGAKSRLTFRMGRGKMIMTETVTVRNLPDEFSGTYEVKNVFNIVKNRFIALSENKTK
                     YISESEFQFKGFMRIIAFFMPGAFKKQSQKYLQLFKEFAEKEGMAK"
     gene            898..1131
                     /locus_tag="PK683_03595"
     CDS             898..1131
                     /locus_tag="PK683_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07561.1"
                     /translation="MNSVIRVVEKLVSTTPFLIFLKNLQLAYARANLRAAAELILPEV
                     SPAWKLLRDYRLLAKLLKMAAGSEAGSAKSETA"
     gene            complement(1431..2456)
                     /locus_tag="PK683_03600"
     CDS             complement(1431..2456)
                     /locus_tag="PK683_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNH07562.1"
                     /translation="MTETEKKLSPVSKFLYGFLILFLSLRCLGTKKSPFDSNSPDGLL
                     RSLIASLFSVPVPETVPLISPAVLTLTYSASSFLLPQNVPFTLTPALSRTVTGCTVSP
                     SLPAGLSIDNRTCTISGTPTVTQTASSYSITAVNSVARTSATIAIAVNEALSFSWGLF
                     TDMVDGTVRMEVNAGTFGGQTYSAQTLYFMKCSQGYTWNSATNGCTGTYGGYTYCNSN
                     DNSCNSDGTGNPPWTLNGTGTSGAYATCNSLNFAGKTGWRVPTLNELKLLIMCNDNIM
                     PSTACGVGNYPSPSTNSLFPNTDGYYYWSSTYQALGGAWHVNFNGGSVAPSTKTNGHH
                     VRCVYGP"
     gene            complement(2723..2947)
                     /locus_tag="PK683_03605"
     CDS             complement(2723..2947)
                     /locus_tag="PK683_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07563.1"
                     /translation="MEYQDEKGENRSALFASPTWDSVKIDEKKIPAQEILPTAYTEDL
                     FDLSASGNNQITYIFFENLKKRLPESRVQD"
     gene            complement(3022..3165)
                     /locus_tag="PK683_03610"
     CDS             complement(3022..3165)
                     /locus_tag="PK683_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07564.1"
                     /translation="MNENISRYLEGRSEQDVFYVPFSNYFQTNIVLLAVNLTAIHTFD
                     SIP"
BASE COUNT          980 a          719 c          736 g         1093 t
ORIGIN      
        1 ttttctttca tttttttgcc gatagaatag ccgattttta atgcgttttg ctgagataac
       61 cgcgcctgcg gaattttcca aaaatgagaa aaaagatcca agtctgcatt tcctttttca
      121 gttaaacctg tcttttttga caggcaggtg catctgaggg ttaaaggaaa cttccacaaa
      181 tttcggcagt tccagcaggg ataccaaagc agtaaaaact gcaggcagct gtctccaatg
      241 ttgtcagtgt caatttagag atttcagaaa gtatgacttc tgtttaaatg aaatgtctgc
      301 cgttattttg ccattccctc tttctctgca aattccttga acagctgcag gtatttttgg
      361 gactgcttct taaatgctcc aggcataaag aatgctataa ttctcataaa gcctttaaac
      421 tggaattcac tctctgaaat atactttgtc ttattttctg aaagtgcaat aaacctgttc
      481 tttactatgt tgaatacgtt ttttacttca tacgttccgc tgaattcgtc cggaagattg
      541 cggacagtta ctgtctcagt cattatcatt ttgccccttc ccatcctgaa tgtaagtctg
      601 gatttggcgc ccggctgacc cggcgttccg ctgagatgct cgaaactcag cagtcccggc
      661 tgccatttct gtaaattatc tggattatca aaaagttcta tgactctggc aatcggtaaa
      721 ttgatttcta tctcaacact gtatttcatt tcacgtcttt acccgaacga ttgtcggttc
      781 ctttattttt aaatttgcag tacagaaaaa tgaggccgaa cggtttgcat ttattcgaga
      841 ctggctctca gttactcaga attttatttg agtcctgaca gtcaactgaa acattgtttg
      901 aactctgtta ttagagttgt tgaaaaatta gtttcaacaa ctccattttt aatttttcta
      961 aaaaacctgc agctcgctta tgcgcgtgca aacttgagag ccgctgctga gttaatccta
     1021 ccggaggttt cgccagcgtg gaagctgcta agggactatc gtctcttagc caaacttctg
     1081 aagatggctg ccggttcgga agctggctcg gcaaaatctg aaactgccta aatccctatt
     1141 tagcttctga tgaaagataa tttcttgatt ttttcaacaa gtctattata aattctatgt
     1201 actatagaat ttaattttct gcttttataa aagcagaaaa atctgttctg ccgagaacct
     1261 gatataatat ggaattcata tcattgagat gatccgatga tcatcagaaa tatgttcctg
     1321 tgttaaagct aatatacaga gtgtcacagt gcttttttta ggcaaatcaa aaatgatgaa
     1381 ttcctttgac agttttcagt ccagaaattt tttcagagat atttttgctg ctacggtcca
     1441 tacacacatc gtacatggtg tccgtttgtt ttggtgctcg gcgccacact gccgccgttg
     1501 aaattgacat gccaggcacc tcccagtgcc tggtaagtgg acgaccaata gtagtaacca
     1561 tcagtattcg ggaataaact gttggtggac ggtgaggggt aatttccgac cccgcaggcg
     1621 gtactcggca taatgttgtc attgcacata atcagcagtt tcagttcatt cagagtagga
     1681 acccgccagc ctgtcttgcc cgcaaagttc aaagaattgc aggttgcata agccccgctc
     1741 gttccggttc cgttcagagt ccagggagga tttcctgtcc cgtcgctgtt gcagctgttg
     1801 tcatttgaat tgcaataggt ataaccgcca taagttccag tgcacccgtt tgttgcagaa
     1861 ttccaagtat acccctgact gcatttcata aaatacaggg tctgagcaga atacgtctga
     1921 ccgccgaatg ttcctgcatt gacttccata cggactgtcc cgtctaccat atccgtaaag
     1981 agcccccagg aaaaagacag tgcctcattt acggctattg ctatcgttgc tgatgtcctg
     2041 gcgacggaat tgactgcagt aattgagtaa ctggaagcgg tttgtgtaac tgtcggggtc
     2101 ccgctgattg tacatgtccg attgtcaatc gacagtccgg cgggaagact tgggctgaca
     2161 gtgcatccag taacggttct ggaaagagcg ggagtcaatg tgaatggaac attctgaggc
     2221 agcaagaatg aacttgctga ataggttaag gtgaggacag caggtgatat caaaggcact
     2281 gtctccggaa ctggaacgga aaacagggat gctatcagtg agcggagaag tccgtcaggg
     2341 ctgttcgaat caaatggaga tttttttgtc cccagacagc gtaatgataa aaacaggatt
     2401 aaaaaaccat aaagaaattt agaaacagga gaaagtttct tttctgtttc tgtcaaaacc
     2461 ttttcctgaa cggaggatat acattcattt tctacagtca tttttcttct ctttccaaat
     2521 agtttgtcgg cgaatgtaaa agtcaatttg agccgcacgc tcagccgtct ctgctcagtc
     2581 agggttttcg gcctgaattg acaattttct tacacacgac aataccactt ttcgtcctga
     2641 aatattttac ttcaactttt tatttccggg tctttgacag cgaagcagcg aagcggctcc
     2701 ttgaattcgg tgctgcagtt tttcagtcct gcactcttga ttcgggaagt ctctttttca
     2761 ggttttcgaa aaaaatgtaa gtgatctgat tattccctga agctgaaaga tcaaacagat
     2821 cttctgtata tgccgtcgga aggatttcct gtgcaggaat tttcttctca tctattttga
     2881 ctgagtccca ggtcggactt gcaaaaagcg cggatctgtt ttctcccttt tcatcctgat
     2941 attccactaa gacgactgga agcagccaga aagaacctct cggattgtaa attacacggt
     3001 atttttttag ggatattttt gttatggaat cgaatcgaat gtatgtatgg ctgtcaggtt
     3061 tacagccaga agcacaatat tcgtctgaaa ataattggaa aaaggaacgt aaaatacatc
     3121 ctgctcagat cggccttcga gatatctgct gatattttca ttcataggtc agtattccgt
     3181 ttggtatctt caggagcaac tgatatgcag attccaaagg cattagaaat tcagtagtcc
     3241 gttccaggca gtaaaggcca gaatcagcta aaattgatat tgacagtata aataatcttg
     3301 gaaaattgat attgacagta taaattatgg aattcaatga actggaataa attgtcctgg
     3361 aacaaggttg aaaaaaaaga ctcaggcttg gaatgtattt atgatctgaa aaaaagaaat
     3421 aaagaaattt actatttata gaataagcat aaatgcgatt ttattcttcc tgatattcac
     3481 gaatgccgtc atactgcaat tgcaatatcc acaaaacgtt ctctggac
//
LOCUS       DAOQGJ010000159         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15141_length_3528_cov_2.988771, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000159 DAOQGJ010000000
VERSION     DAOQGJ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15141_length_3528_cov_2.988771"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="PK683_03615"
     CDS             <1..1255
                     /locus_tag="PK683_03615"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07565.1"
                     /translation="QRIFKSFGMYFSGISFLKQTTAAILLYGVKDAVFSLHWDVHCPL
                     CDMVTHHYSSLHSMKNKSKCRMCNQEFQPDFAERIEVSFSMHPEIENIDIPPFCPPPD
                     SLKVYAETGAEFGQIGETETVLRTGIYRYFCPITNAKGVLTVSGEETQDLQEMKIHQK
                     EDAYEETSFSLRPGRIRIEITNHKVPACGVFVIDENLSEAIPFEKLSSRLTGLQLIHI
                     PEFQELFGDEILSDREGLVISSVTILFTDITGSTKMYEELGDIKAYNIVRDHFEILAH
                     CIENEGGTVIKTIGDAVMASFLSPEKAVNAVFDAVRQFQKYNEPKSVKEQIYLKIGIH
                     SGPAILVNLNERTDYFGTTVNTAARIQSMSEDNLFCVSENVFRNPNVIAVMKKYGIHR
                     VKKSDRELKGLSGKYPVYFIPMILE"
     gene            1354..2535
                     /locus_tag="PK683_03620"
     CDS             1354..2535
                     /locus_tag="PK683_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07566.1"
                     /translation="MNRINRKTFLIGSGVMLTVPAFLQNCSSVPGRLYGKSGSDPLEN
                     ADAEGFDKPIHRVINAGITAPNSHNVQPWKFRILSDTEMILYADEKRLLPVTDPPARQ
                     IHISHGCFLEVLKIGASQIGYDAEIKILPEGDYSFSEIGKKPSAHIRLRRSSKSGHPL
                     YDAVRKRATDRSEYKGDYITEKQTDEMKKLGNSVHSSLHFILGRENISSLMNIFYESM
                     KLESFTYRTNDETRTWFRFNEKEIQTKRDGLSLPGQGVTGFTRWVAETFFMGPEPEKF
                     HNRGGLDMFMERYRNNIGSAKGLVYWKTAGNTKKDWIHTGMDYARFQLALTSLGYKMH
                     PMSQPLQEFPEMDSYRKALDSALSVKPSEKIQMIARLGKSSFAYFSPRRSQKDMLLIP
                     V"
     gene            2584..3198
                     /locus_tag="PK683_03625"
     CDS             2584..3198
                     /locus_tag="PK683_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HNH07567.1"
                     /translation="MMKKGDLFFLIQSFFIQVISEALMTADEINLEMFKEEMEKRFSF
                     KSRGVSTLLIPASFMKHFEIQSKIFRGKPEYLSYLLRRFRIAMRSFARDPEGLKNLYQ
                     RKNQDLKKVNLRPFDEDWAELGSFSVASGRSRCLLFVILMLFDMGGFGSALKQAGIVM
                     NEPFSPEVSWELIGKFGVEREAGYCIRGFEPRQTKGKTEQEKLE"
     gene            3195..>3528
                     /locus_tag="PK683_03630"
     CDS             3195..>3528
                     /locus_tag="PK683_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07568.1"
                     /translation="MKGRIAFILITFSIFFIIIKYVSIRSIIGKFFSKKSTSDVLNFL
                     EPKVRLKYAKIFSAANLSFPPRKIILTAYKKEKVLELWAKDEDNYKFIYQFEIKKASG
                     KPGPKLREG"
BASE COUNT         1089 a          642 c          798 g          999 t
ORIGIN      
        1 tcagagaata ttcaagtcct tcggaatgta tttttcaggc atctctttct taaagcagac
       61 aactgcagca attcttcttt acggagtaaa ggatgctgtc ttcagcctgc attgggatgt
      121 gcactgtcct ctatgtgata tggtcactca tcattacagc agtctgcact caatgaagaa
      181 caaatcgaaa tgcaggatgt gcaatcagga gtttcagcct gattttgcgg aaagaataga
      241 ggtcagtttt tcgatgcatc ctgaaatcga gaatattgat atcccccctt tctgtcctcc
      301 gcctgacagc ctcaaagttt atgcagagac aggtgctgaa ttcggacaga tcggtgaaac
      361 agagacagtt ctccgtacag gaatttacag atatttctgc ccgattacaa atgccaaggg
      421 ggttctgact gtttccggcg aagaaactca ggatcttcag gaaatgaaaa ttcatcagaa
      481 agaagatgca tatgaagaaa catctttttc actcaggcct gggagaatca ggatagagat
      541 tacaaatcat aaggttcctg cctgcggggt atttgtcatt gatgagaatc tttctgaagc
      601 cattcctttc gaaaagctct cctcacgtct caccggtctt caattgattc atatccctga
      661 gtttcaggaa ctcttcggag atgaaattct ttcagacaga gaaggactcg taatttcgtc
      721 cgtgacaatc ctgtttactg atattaccgg ttcgacaaaa atgtatgagg agctgggtga
      781 tataaaagct tataatattg tcagggatca ttttgaaata cttgcccact gcattgaaaa
      841 tgaaggggga actgtgatca agacgatcgg ggatgcagtg atggcatcct ttctgtctcc
      901 tgaaaaggca gtgaatgctg tctttgatgc agtcaggcag tttcaaaaat ataatgaacc
      961 caagtctgtg aaagagcaga tttatcttaa gataggaatt cattccggtc ctgcaatact
     1021 tgtgaatctg aatgaaagga ccgattactt cgggacgacc gtcaatactg cagcgaggat
     1081 tcagtctatg tctgaagaca atctcttctg tgtttctgag aatgtatttc gaaatcctaa
     1141 tgtgattgct gtaatgaaaa aatacggaat ccacagagtg aaaaaatcag atcgggaact
     1201 gaaaggactt tccgggaaat atccggttta ttttattccc atgattctgg aatagatgct
     1261 atcggatcag tcagaggaat gaaaggatat agtgtaattt ttttaaatta aattttaaac
     1321 aatcagcata aacagagaga aaggacggaa gtcatgaatc gcataaacag aaaaaccttt
     1381 ttaatcggct cgggagtgat gctcacagtg cctgcatttc tgcagaactg cagttcagtt
     1441 cctggaaggc tttatggaaa atcaggctct gatcctcttg aaaatgctga tgccgaggga
     1501 tttgataagc cgatacacag agtgatcaat gcaggaatca ctgctcccaa ttctcataat
     1561 gttcagcctt ggaagttccg aattctgtca gatacggaaa tgattcttta tgcagatgaa
     1621 aaaagacttc tgccggtgac agatcctcct gcaagacaga tccatatcag ccacggatgt
     1681 tttcttgagg tactgaagat tggagcatca cagatcggat atgatgctga gataaaaatt
     1741 ctgcctgaag gggattattc cttttcagaa atcggcaaaa aaccatcagc acatatcaga
     1801 cttcgacgca gttctaaatc agggcacccg ctgtatgatg ctgtaagaaa aagagctaca
     1861 gacaggtctg aatataaggg ggattacatc actgaaaaac agacggatga gatgaaaaag
     1921 ctcgggaatt cggttcattc cagtcttcat tttatacttg ggagagagaa tataagttca
     1981 ctgatgaata ttttttatga atcaatgaaa cttgaatcgt tcacatacag aaccaatgat
     2041 gaaaccagaa cctggttccg ttttaatgaa aaagagatac agaccaagag agacggtctt
     2101 tctcttcccg gacagggagt caccggcttt accagatggg tggcagagac attttttatg
     2161 ggacctgaac cggaaaaatt tcataacagg ggcggtctgg atatgtttat ggaaagatac
     2221 agaaataata tcggatcagc caaggggctt gtctactgga aaacggctgg caatacaaaa
     2281 aaagactgga tacatacagg aatggattat gccagatttc agcttgcact gacttctctc
     2341 ggttataaaa tgcatccgat gagtcagcct cttcaggaat ttccggaaat ggattcttat
     2401 cgcaaggccc ttgattctgc cctgtctgtg aagccttccg aaaaaattca gatgattgcc
     2461 cgtcttggaa aaagcagttt cgcctatttt tcccccagac gctctcagaa agatatgctg
     2521 ctcattcctg tgtaggatct cctaaaagaa taaaatttgc attcttgtgc aaaaagagtg
     2581 cttatgatga aaaagggtga tctttttttt ctgatacagt catttttcat acaggtcatc
     2641 agtgaggctc tgatgactgc tgatgaaata aatttggaaa tgttcaaaga ggagatggaa
     2701 aaaagatttt cctttaagag tagaggagtt tccaccttgc tgattcctgc ttcgtttatg
     2761 aagcattttg aaatacagtc aaaaattttt cgcggaaaac ctgaatacct gtcatatctc
     2821 cttaggcgat ttagaattgc aatgaggagc tttgcccgag atcccgaggg attgaaaaac
     2881 ctgtatcaaa gaaaaaatca ggatctaaaa aaagttaatt taagaccatt tgatgaggat
     2941 tgggctgaat taggttcttt ttcggtggct tcaggcagaa gtcgatgcct tctttttgtt
     3001 atccttatgc tttttgatat gggaggtttt ggttcagcac ttaagcaggc tggaatcgta
     3061 atgaacgagc ccttttctcc cgaagtttcg tgggaactca tcggtaaatt cggagtggag
     3121 cgggaggcgg gatattgtat tcggggattt gagccaaggc agacaaaagg aaagactgag
     3181 caggagaaat tggaatgaaa ggcaggattg catttatatt aattacattt tctatattct
     3241 ttattattat aaagtatgta agcataagaa gtattatagg aaagttcttt tctaagaaga
     3301 gtacatcgga tgtcctgaac tttcttgaac ctaaggtgag acttaaatat gcaaagattt
     3361 tttctgcggc gaatctttct tttccgccgc gaaaaatcat tttaactgca tacaagaagg
     3421 agaaggttct cgagctttgg gcgaaagatg aagacaacta taaatttatt tatcaatttg
     3481 aaataaagaa agcaagcgga aagcccgggc ctaagcttag ggagggcg
//
LOCUS       DAOQGJ010000160         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15146_length_3527_cov_3.704781, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000160 DAOQGJ010000000
VERSION     DAOQGJ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15146_length_3527_cov_3.704781"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..318)
                     /locus_tag="PK683_03635"
     CDS             complement(157..318)
                     /locus_tag="PK683_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07569.1"
                     /translation="MTDSEVKDKIEKLKRQQAKPKEQLEYAKADIEKEFSSGKSASTK
                     KISVFCIWK"
     gene            complement(315..593)
                     /locus_tag="PK683_03640"
                     /pseudo
     CDS             complement(315..593)
                     /locus_tag="PK683_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019227.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
     gene            complement(650..847)
                     /locus_tag="PK683_03645"
     CDS             complement(650..847)
                     /locus_tag="PK683_03645"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07570.1"
                     /translation="MPQKWVFCGTYRIWRKRIKKLDIGNSDFKSLIENDNYYVDKSLF
                     IQELIDIQKQVILIPRPRRFG"
     gene            complement(888..2387)
                     /locus_tag="PK683_03650"
     CDS             complement(888..2387)
                     /locus_tag="PK683_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HNH07571.1"
                     /translation="MKKVILLILLFSNCMIFPGFGTITGVEAKKRVMDAANKTDRVIL
                     AAFVGAEYAPLADMLNSMIVPALISIEEGKNYDEDSVKDCEKKIASIAGLVLGSSMTI
                     PSCNIQEAGLVDIGPLEIGGGKKAKTNKLLAFLLNFSGTSPSSTSTSTSAGNTSTDTS
                     TGTFTGTGTGVSPPACTTSGAADAVKYDLAFSVADAEYSRTADRMVIIDSAGRFHYYN
                     PSANSDTSLNLNFSPLNVTVSPDGTKAAVGHDTKVTLINLPAATIAREVSTAVTARDI
                     VMNNTYAYVSNAAYSWNVIKSITFSDGTVSNQTGTTNYPLNMVLNSAGNSIYAVSNGL
                     SPADIVKFNITSNPPAYLYDSFYHGDYSMCSPIWIAKDASRLFTACLNTFTVSAVQAS
                     DINYAGKINGLTKIVHLDTDSSKIYLIPTVFSSWDIGDSSEYIHLYNLSNLSFISKKT
                     LPCYVSGGVSYKTNGKFVFANNAGTEYYVIAKPDPNSGVLNDVVYKFSL"
     gene            2474..2788
                     /locus_tag="PK683_03655"
     CDS             2474..2788
                     /locus_tag="PK683_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07572.1"
                     /translation="MQSEILHNGDTLHYTAVPNDPKQVPQKGTCTLSKPDSNGHTSKG
                     YEGQVIPKETELSVIARTKTKAKVQKWENYWYYVEKRSHNLVADWITSCGPGWVYGDS
                     LL"
     gene            complement(2891..3019)
                     /locus_tag="PK683_03660"
     CDS             complement(2891..3019)
                     /locus_tag="PK683_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07573.1"
                     /translation="MSRLKAVHSGDLLDIPAEGGMTQILQGDKGFEDYGDVTLQEG"
     gene            complement(3020..>3527)
                     /locus_tag="PK683_03665"
     CDS             complement(3020..>3527)
                     /locus_tag="PK683_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07574.1"
                     /translation="SLEINVVFLCDATTIIYETLHAYGAVDLYNVNGTHEEEKVENYT
                     AENFPNEVMLCSASMEEPDISGFNAFMVGWHREHDDWFFDMVTEESRSWLQEMIDNVE
                     NFDEDGNLIIDTMEEVAVYTSEDGKEFHRYQINGTESIYLWREYGQDDDKDAEGKEYQ
                     EFFLYDED"
BASE COUNT          965 a          804 c          681 g         1077 t
ORIGIN      
        1 ccgtgacttt gggaactgct tctttagatt ccccgatcgg ctcaaaggaa atggtatctg
       61 tgacaggata actttccatc aactctcttt ctattttgga aatccggttc tctatgtccc
      121 tgccattaat tttttcacct ttcattgatg cggccgtcac ttccaaatac agaagactga
      181 tatctttttg gtacttgctg attttccgga actaaattct ttttcaatgt cagccttggc
      241 atattccaac tgctctttag gtttggcctg ctgcctcttt agcttctcaa ttttatcctt
      301 cacttcagaa tcagtcaaat accccaaccc tttttcatat tgtcttctgc ttcttttttt
      361 agtttctcgt attcagcttt ctcttcccct gtcattaata cttcttgaaa aatatttttc
      421 ttttcataat tatgcaccca gacaccacct gcgaagtagc tgtgggttac ctcgacttcg
      481 aaattgaaca ccttcgtttc aggaacatga agaacctcga cagatgcgac agtgacattt
      541 tttccatgag aattcatcag actgtatcct ttcctgatgt cctttgcctc agtccatttt
      601 ccggttttcc tttttcaaag aaatatttca gcatcagcag attcatcgtc tatccaaatc
      661 tgcgagggcg gggaataagg atcacctgtt tctgtatatc aatcagttcc tgaatgaaaa
      721 gagatttgtc cacatagtag ttgtcattct ctatgaggct cttgaagtca gagttgccga
      781 tatccagttt ttttatgcgt tttctccaga ttctgtatgt cccgcagaat acccacttct
      841 gcggcaatgt ccctcttgag ttcggaaagc ggacgtttca gaaaacctta caatgaaaat
      901 ttatatacta catcattcag aactcccgaa ttgggatcag gttttgcgat aacgtagtat
      961 tctgtaccgg cattgttggc aaaaacaaat ttcccgttcg tcttatagga aactcctccc
     1021 gaaacataac aaggaagtgt tttcttggaa ataaagctga gatttgacag attataaaga
     1081 tgtatatatt cactggaatc accgatatcc caggaagaga aaacagtcgg gatcagataa
     1141 attttagatg agtcagtatc cagatgaaca atttttgtca ggccgtttat cttcccggca
     1201 taattgatgt ctgacgcctg aaccgcactg actgtaaatg tattcaggca ggcagtgaaa
     1261 agacggcttg catcttttgc aatccagatg ggactgcaca tactgtaatc tccatgatag
     1321 aaagaatcat aaagatatgc gggcggattt gacgtaatgt tgaacttcac aatgtccgca
     1381 ggcgacagtc cattgctgac tgcataaatc gaattcccgg cagaattcaa aaccatgttc
     1441 agaggatagt ttgttgtccc cgtctggttg gatacagttc catctgaaaa tgtaatgctt
     1501 ttgatcacgt tccaggaata tgctgcattg gatacataag catacgtatt gttcattacg
     1561 atgtctcttg ctgtaacagc agtgctcact tctctggcaa tggtagctgc aggaagattg
     1621 atcaatgtga cttttgtatc gtgtcccact gcagcctttg tcccgtccgg actgacggtg
     1681 acattcaaag gtgaaaaatt caaattaaga gaagtatctg aattggccga gggattgtaa
     1741 taatgaaaac ggcctgcaga atcaatgatc accattctgt cagcagttct gctgtattct
     1801 gcatctgcca ctgaaaatgc caagtcatat ttaactgcat cagctgctcc tgaagttgtg
     1861 caggcaggag gggaaactcc agttccagta ccagtgaaag taccggtaga tgtgtctgta
     1921 gatgtatttc ctgccgaggt ggaggtagaa gtggaagatg gactggtacc tgagaaattc
     1981 agcaggaatg ccaataattt attggttttt gcctttttcc ctcctccgat ttcaagaggt
     2041 ccgatatcaa ctagtcctgc ttcctgaata ttacagctgg gaattgtcat tgaactgcct
     2101 aaaaccagcc cagcgatgga agcgattttc ttttcgcagt cttttactga gtcctcgtca
     2161 tagtttttcc cctcttctat actgatcagt gcaggcacga tcatactgtt cagcatatcc
     2221 gcaagtggag catactctgc accgacaaat gcagcaagaa tcacacggtc agttttgttt
     2281 gctgcgtcca tcaccctttt tttggcctca actccggtaa tagtgccaaa accgggaaaa
     2341 atcatacagt tggaaaataa caaaatcaat aaaatcactt ttttcatttc actttgcctc
     2401 ttctgacact tatgaattag aataactgcc tgtaaagctt tttttaaaag gaaatctcgt
     2461 aaataaatgc tttttgcagt cagaaatact gcacaatgga gacacactgc actatactgc
     2521 agtgcccaat gacccaaaac aggttccgca aaagggcacc tgcactttga gcaaacccga
     2581 cagcaatggc catacatcca agggatatga gggtcaagta ataccgaaag aaacagagct
     2641 gtctgtcatt gccagaacga agaccaaggc caaggtacag aaatgggaaa actactggta
     2701 ctatgtagaa aaaagatcgc acaaccttgt ggcagactgg ataacatcct gcgggcctgg
     2761 ctgggtgtat ggggattcat tactttaaaa tgattcactc accttacgca tttcgtgctt
     2821 acagtgagta attcagacat cttttctgaa aaaacgcttc agtgaaatta aaaatataaa
     2881 aaaatatttt ttatccttcc tgaagtgtca cgtcaccgta gtcttcaaat cccttgtctc
     2941 cctgcagaat ctgagtcatt cctccttctg caggaatatc cagaagatcc ccggaatgga
     3001 cagccttcag tctggacatt cagtcttcat catacagaaa gaattcctgg tattccttgc
     3061 cttctgcatc tttgtcatcg tcctgaccgt attctctcca gagataaata gattctgttc
     3121 cattgatctg atagcggtga aattctttgc cgtcctctga tgtatataca gccacctctt
     3181 ccatagtgtc gatgatcaga tttccatctt cgtcaaagtt ttccacattg tcgatcatct
     3241 cctgcagcca ggatctggat tcctcagtga ccatatcaaa aaaccagtca tcgtgttccc
     3301 gatgccatcc caccataaag gcattgaatc cggaaatatc cggctcttcc atacttgcgc
     3361 tgcagagcat aacctcattc ggaaaatttt ccgcagtgta attttctact ttttcttctt
     3421 catgtgttcc gttcacgttg tacaggtcga ctgccccata ggcatggaga gtctcatata
     3481 taatcgtagt cgcatcacat aaaaatacaa cattgatttc cagactg
//
LOCUS       DAOQGJ010000161         3525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15162_length_3525_cov_3.536599, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000161 DAOQGJ010000000
VERSION     DAOQGJ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15162_length_3525_cov_3.536599"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3525)
                     /locus_tag="PK683_03670"
     CDS             complement(<1..>3525)
                     /locus_tag="PK683_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07575.1"
                     /translation="VICLRLGEKQKFEKEIREAYYTASEYQFEREMLSIYLIQAEYYY
                     LEGKSARTLELVLNAETLLKNSPGLFYSANELFLFFLFALEVNGNMNLGNMDKLRISR
                     EKLYSAIFFRQLLVNELKFQDYSLFDTLNDVQFYALEDREYSRKYESAVLKKMSTKDI
                     IAGKQKNLQKFNEKFDEFLSLLPEGMDVSSWNRTPPKLELPNKKDEMIIELYTNGKDY
                     VQNAYYMGKRITEKYTVKDRYNLEDISAGLNKILVSRPEVKSIVIIPAPILFRINFSK
                     LPYEGTELGKKYKIRHLFRMSELVRESQSEFSRLKRITSINTQKKEEDKKWVGKIIDS
                     SIGRLVRPVEISKDKSKEFNLRIVEFDNIRNYLMDSDIVEGAVDFLNRKHYLGEKKFG
                     HINMKEIAENQWSISTVIVNNYKRTIDNYVQIAFLFDILQFAGVQSIVLLEEDREDLV
                     QTRNELISNLSSAGKIIQEKGLTIVGEQINPYQHSEKEFEAEYGRLMKLSAGEERNKR
                     YLNAIRYQLQANSVLTENRPELVIRSELNIARLKTYLFVSRNYLQHYETLLSKYSAGT
                     SEEEKILYEMLIRCYESSLEVKCENYFERHQVHPGSTDDRKFITRYYRALRRGDLTVV
                     DPEYRRFISIPFTEDRYSTHLKLAYLFSRGFIWDKAVFHAREAGKLADTETEKKYSEL
                     ILSDIEYEIFFIKGIDPNIQTKEKIYYFATSRSWAKYREKINNMLQYENNYFKKNYQT
                     RIYEAFEHLETNSDFEPVSLGPLFLKDGRTSLYLLKEPDRNFLFHLLLKSIQTQLGNE
                     LNNQFDFLIETEESLNNRNRSLWMKLQWASALYRRGDMDGAKKYFSLFERNFEEYYPE
                     KSITMSYFFLRFKLSKIFKDISFSEKERKFVQENFPDWFRYYEEADQESESRNYLNLV
                     KKIIREKAKEKLDIYNSNEMNDFFYYLMKEAHDRSDWVSFLELSYWKEKFKAFNDKIG
                     GKFFTFGEIPAVRMNTVQTLLDRLPADQSFKAMADLGIKTILLSSSGKKFEAKEVFND
                     NRRIKFRILDYIFTVKNGGTSIVKQESLENRYRTALGLEKNKLTYLYMPSYHFKALLE
                     PEELDHYYYVLDLESLAEKKMYSAVNDTQKSFNVKTLPTSVQETKAKRILHQLENLEI
                     KSVTDRESAFPFIVSQENLKLYEGRQILF"
BASE COUNT          951 a          768 c          619 g         1187 t
ORIGIN      
        1 aaaaagaatc tgccttccct cataaagttt cagattttcc tgagatacaa taaacggaaa
       61 tgcagattct ctgtccgtta ctgatttaat ttccaggttc tctagctgat gaagaattct
      121 cttagctttg gtttcctgca cagaggtcgg gagtgttttc acgttgaatg atttttgagt
      181 atcattcacg gcactgtaca ttttcttttc agcaagagac tcaagatcca gcacataata
      241 ataatgatcc agttcttccg gctcaagaag tgccttgaaa tgataagagg gcatataaag
      301 ataagtgagc ttattttttt caagaccgag agctgtcctg tatctgttct ccagactctc
      361 ctgctttaca atgcttgtcc cgccgttttt tacagtgaat atataatcca gaattctgaa
      421 tttgatcctt ctgttatcat tgaatacttc ctttgcttca aattttttcc cgctgctgga
      481 aagaagaatt gtcttaatgc ccagatctgc cattgccttg aaactttggt cggcaggaag
      541 cctgtcaagc aatgtctgca ctgtattcat ccgtactgcc gggatctcac cgaatgtgaa
      601 aaactttcct ccaatcttgt cattaaatgc cttgaatttc tccttccaat aagacagctc
      661 aagaaaactg acccagtcag acctgtcatg agcttctttc atcagataat aaaaaaagtc
      721 attcatttca tttgaattgt aaatatccag cttctctttt gctttttctc tgataatttt
      781 cttcacaaga ttcagatagt ttctgctctc tgactcctga tccgcttctt catagtatcg
      841 aaaccagtcc ggaaaatttt cctgtacaaa ttttctttcc ttctctgaga atgaaatatc
      901 cttaaaaatt tttgagagtt tgaatctcag aaaaaaatat gacattgtaa tgcttttttc
      961 aggataatac tcttcaaaat ttctttcaaa aagagaaaaa tatttcttgg cgccgtccat
     1021 atctccgcgt ctgtacagtg cagatgccca ctgaagtttc atccagagtg acctgttcct
     1081 gttgttcagg ctctcttctg tttcaatcag aaaatcgaac tggttgttca gttcgtttcc
     1141 aagctgagtc tgaatggact tcagaagaag gtgaaacaga aagtttctgt caggttcctt
     1201 gaggagatac agtgaagtcc tcccgtcttt cagaaagaga ggtcccagtg atacaggttc
     1261 aaagtcggag tttgtttcca gatgttcaaa tgcctcataa atcctggtct gataattttt
     1321 cttgaaataa ttattttcat actgaagcat attatttatc ttctcgcgat atttagccca
     1381 ggatctggag gtcgcgaagt aataaatttt ttccttggtc tgaatattcg gatcaattcc
     1441 cttgatgaaa aagatttcat attcaatatc agagagaatc agttcggaat actttttttc
     1501 tgtttccgtg tctgcgagtt tgcctgcttc tctggcatga aacacagcct tatcccagat
     1561 aaaacctctt gagaaaagat aggcaagctt caaatgggtg gaatatcgat cctctgtgaa
     1621 cgggatggat atgaatcttc tgtattcagg atcaactaca gtcaaatctc cgcgtctaag
     1681 tgccctgtag tatcgggtaa tgaattttct gtcatcggtc gaacccggat gcacctgatg
     1741 tctttcgaaa taattctcgc atttcacttc cagggaggac tcataacatc tgatcagcat
     1801 ttcgtaaaga atcttttctt cttccgaggt tcccgcagag tactttgaaa gaagggtttc
     1861 atagtgctga agatagtttc tgcttacaaa aagataggtc ttcagtctgg caatattcag
     1921 ttcagaacgg attacaagtt caggtctgtt ttccgtaagc acagagtttg cctgaagctg
     1981 ataacgtatt gcattcaggt atcgcttgtt cctttcttcc ccggcagaca gtttcatcag
     2041 ccttccgtat tctgcctcaa actccttctc tgaatgctga taaggattga tctgctcgcc
     2101 gactatcgtg agaccttttt cctggatgat ctttcctgca ctgctcagat ttgaaatcag
     2161 ttcgtttctt gtctgaacca gatcctccct gtcctcttca agaagaacaa tagactgaac
     2221 tcctgcaaac tgaagtatgt caaaaaggaa ggcaatctgt acataattat caattgttct
     2281 tttataatta ttcactatta cagtagatat gctccactga ttctctgcaa tttccttcat
     2341 attgatatga ccgaatttct tttcacccag atagtgtttt ctgttcagaa aatcaaccgc
     2401 cccctcgaca atgtctgaat ccatcagata attcctgata ttgtcaaatt caactattct
     2461 taaattgaac tcctttgact tgtcctttga aatttccaca ggacgcacaa gtctgcctat
     2521 gctgctgtca atgatctttc caacccactt cttatcttct tctttcttct gagtattgat
     2581 gcttgtaatt ctcttcagtc tggaaaactc gctctgactt tctcttacaa gttcagacat
     2641 cctgaacaga tgcctgattt tatatttttt accaagctcc gttccctcgt aaggaagctt
     2701 gctgaaattt attctgaaaa gaataggtgc aggaatgatg acaatgcttt tcacttcagg
     2761 acgggagact agaatcttat tcagtccggc agatatatcc tccaggttgt atctatcctt
     2821 tactgtatat ttttcagtaa ttcttttacc catataataa gcattctgta cataatcctt
     2881 cccgttggta taaagctcaa tgatcatctc gtctttttta ttcggcagtt ccagctttgg
     2941 aggagttctg ttccagctcg aaacatccat tccttcagga agcagtgaaa ggaattcatc
     3001 aaatttctca ttaaatttct gaaggttttt ctgctttcct gcaataatat cctttgtgct
     3061 catctttttc agaacagcag actcgtactt tctggaatac tccctgtctt ccagagcgta
     3121 aaactgaaca tcattcaatg tgtcaaagag actgtaatcc tggaacttca gttcattgac
     3181 cagaagctgc cgaaagaata tagcgctgta cagtttttcc ctggaaattc tcagcttatc
     3241 catatttcca agattcatat tcccgttcac ttccaaagca aaaagaaaaa acaggaacag
     3301 ctcatttgcg ctgtagaaaa gaccaggact gtttttcagg agtgtttcag cattcagaac
     3361 cagctcaaga gttctggcac tttttccttc aaggtaataa tactctgcct gaatgaggta
     3421 tatgctcagc atttctcttt caaactgata ttctgaggct gtgtaataag cttccctgat
     3481 ttccttttca aacttctgct tttctccaag tcgaaggcag atcac
//
LOCUS       DAOQGJ010000162         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15213_length_3519_cov_3.418880, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000162 DAOQGJ010000000
VERSION     DAOQGJ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15213_length_3519_cov_3.418880"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /gene="murA"
                     /locus_tag="PK683_03675"
     CDS             <1..1200
                     /gene="murA"
                     /locus_tag="PK683_03675"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004492375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNH07576.1"
                     /translation="GAVCMVKSAVLENLPEINDVLMLLDVMKAIGFTVIKKSPNTYHF
                     QRPDELTGSLPVELCSRIRGAVTLAGPILAMTGRVFLPRPGGDKIGRRRMDTHLYALQ
                     ALGAHIEVFPDGYEISAQRLIGADILLDEASVTATENAVMAASLAQGTTILRNAASEP
                     HVQALCRFLNSAGAKISGIGTNVLTIEGVTSLGNQQITHRIGSDYLEVGSFISLAAVT
                     GGEIFIKDVNPDDIRMIRLVYSRLGIEVRFQDNGVLVPRDQKMEVIPDYHGATMKIDD
                     SPWPGFPADMTSVALVTATQCQGTVMIHEKMFESRLFFVDHIIGMGAHIVLCDPHRAL
                     VIGKSKLYGQKVASPDIRAGMAMIIAALCAEGTTQIHNIGQIDRGFQEIDIRLRMLGA
                     QIERLTE"
     gene            complement(1201..1866)
                     /locus_tag="PK683_03680"
     CDS             complement(1201..1866)
                     /locus_tag="PK683_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07577.1"
                     /translation="MYLGLLIQDSVLYFWGFILFLCICGLVFFIYHQFLIPYMELKSN
                     EVETPPDPGSVYEFIVSELDRSAVFTVGKNTGNIITKCSAIQDDHLVFQFKKHPTNEE
                     YDIIIKKNGSVLIKPPRMNLYSVLQGSEKLESHEIIGKSAEFRIGGKIVKERLPSYME
                     LSLTAKFTTNRSGKERLQFIFTIVKFHPGLNLNLPDTQGNFPFGKEDQFSKREAVSEI
                     EEI"
     assembly_gap    1955..1964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2702..3103
                     /locus_tag="PK683_03685"
     CDS             2702..3103
                     /locus_tag="PK683_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH07578.1"
                     /translation="MLRKKLNVKQYDMAKSLGVSPSYLSKIETGAQEPTKKFKTNCAK
                     YLNTSVEKLFSEGTVEEVYPEFSEGLNNILWAKRRELGIKQREMAKKLKVSTPFLSKV
                     EMGLIEPTEEFKKSVSKLLKVDARDLFISTN"
     gene            complement(3232..>3519)
                     /locus_tag="PK683_03690"
     CDS             complement(3232..>3519)
                     /locus_tag="PK683_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07579.1"
                     /translation="PLDKVVEISKDVTSDSMLLLLSNYVSRKERISAIMESYEDKKIR
                     NILITSVNVKQTDIIEDAYENGKPALKERLKNIILSLEDKEQRETLSKIIG"
BASE COUNT         1018 a          699 c          760 g         1032 t
ORIGIN      
        1 ggtgcagtct gtatggtaaa gtcggcagtg cttgaaaatc ttcccgaaat caacgatgtt
       61 ctgatgcttc tggatgtgat gaaggccata ggatttactg taattaaaaa atctcccaat
      121 acataccatt ttcagagacc tgacgaactg acaggcagtc ttcccgttga actctgcagc
      181 cgtatcagag gagcagtcac gttggctgga ccgattcttg caatgactgg acgtgtattt
      241 ctgccaagac cgggcggtga caagatcgga agaagaagga tggatactca tctctatgca
      301 cttcaggcac tcggtgccca catcgaagtt ttccctgacg ggtatgaaat cagtgcacag
      361 agactgattg gtgcagatat tcttctggat gaggcctctg tcactgccac ggaaaatgca
      421 gtgatggctg cttcattggc tcaggggacg acaatactca gaaatgctgc ctcggagccg
      481 catgtgcagg cactgtgcag attcctgaac tcagccggtg caaaaatttc cgggattgga
      541 acgaatgtac tgacgataga aggtgtcaca tctttaggga atcagcagat tactcacaga
      601 atcgggtcag actatctgga ggtgggcagt tttatcagtc ttgctgcagt gactggcggt
      661 gagattttta tcaaggatgt aaatcctgac gatattcgaa tgatccgtct agtgtactcc
      721 agactgggca tagaagtgag gtttcaggac aatggagtgc ttgtgccgag ggatcagaag
      781 atggaagtca ttcccgatta tcatggagct acaatgaaga tcgacgactc cccctggccc
      841 gggtttcctg cggatatgac ctccgttgcc ctggtgacag ccactcagtg tcagggaaca
      901 gtaatgattc acgaaaagat gtttgagtcc agacttttct ttgtggatca tatcatcggt
      961 atgggtgctc acattgttct gtgtgatcct cacagagctt tagtcattgg aaaatcgaag
     1021 ctttatggac agaaggtggc ttctcccgat attcgggcag gtatggcaat gatcattgcc
     1081 gctctctgtg cagaaggcac aactcagatt cataatatcg gtcagataga caggggcttt
     1141 caggaaattg acatcaggct cagaatgcta ggtgctcaga tcgaaagact gacagaataa
     1201 tcagatttct tcgatttcgg atactgcctc tctcttacta aactgatctt ccttgccgaa
     1261 tggaaaattg ccctgcgtgt caggaagatt caggttcagg cctggatgaa attttacgat
     1321 tgtgaaaatg aactgaagtc tttcctttcc tgatctgttc gtagtgaatt tagcagtcag
     1381 tgaaagttcc atataactgg gcagacgttc tttcactatt tttcctccaa ttctgaattc
     1441 ggccgatttc cctataattt catggctttc cagtttttcc gatccctgca gaacggaata
     1501 cagattcatt cgaggcggct taatcagcac agaaccgttc tttttaatga taatatcata
     1561 ttcttcgttg gtcggatgtt ttttgaactg aaaaaccagg tgatcgtcct gaattgcaga
     1621 acattttgta ataatatttc ctgtattttt gcctacggtg aagactgcag atctgtccag
     1681 ctcgcttacg atgaattcat agactgatcc aggatcagga ggtgtttcca cttcatttga
     1741 cttcagttcc atataaggaa tcaggaactg atggtagata aagaacacaa gtccgcagat
     1801 gcacagaaaa agaatgaatc cccagaaata cagaacggaa tcctgaatta atagacctaa
     1861 gtacatctgc ccgcctcaat caattttgta aaaactttat gcagatcatc tgtcttacag
     1921 acgcatctgt ctttatcatc ggaaaaaaac tttcnnnnnn nnnntaaata aactgcagtt
     1981 tttttcaaaa aagcagggac gaaaccggca aagcaggata tcacgaaatt cgctcctgct
     2041 ccggaaagta catattaaac gatcttcagt gatctgattc agcagttttt ctttctgcgg
     2101 cttataaact ctgccggagt gcagggactt ttgcggaagc aggcagtcgg acagattttt
     2161 tcctgccaga acagatttcc tgtacagact tttcatcagc cctctgatga taaaagtcag
     2221 tatatctcaa tttgagaaag gcatttctgc attcagatta taagttcctg actgtttttt
     2281 tgtgcaaaaa agattcggca tattccaaaa aacttttctt ttcttttttt ttccgtaaaa
     2341 aaagttttta aagagaaaat attcttctga tgcaatgctg agatgctttt catgaaatgc
     2401 attaaaccct ttattttcag acctcctgaa gagtgtttta tttatagtaa taaaaattat
     2461 aaaattttgt ataatcattc cgctcattgg caggatattc cctgaaggag gtgaaattcc
     2521 ccactgctga cttcgaaagg aaatctgccg ggtaagtgta tttgaattat ttgataatga
     2581 aaatcagtat tgatatgtgt caagggttcg gaaactgccg attgtacaaa aattgttgaa
     2641 tgtatggaag ttcaaaaaat tatgtccgca ggggtatata tacatgaaaa accacatcta
     2701 catgctcaga aaaaaattga atgtaaaaca atatgatatg gctaaatcac tgggagtctc
     2761 cccaagctat ctgtctaaaa ttgagactgg tgcccaggaa cctactaaga agttcaaaac
     2821 aaactgtgcc aagtacctga atacttcagt ggaaaaactt ttcagcgaag gaacggttga
     2881 agaagtttat cctgaatttt ctgaagggtt gaacaatata ctttgggcaa aaagaagaga
     2941 acttggaatt aagcagagag aaatggcaaa aaaactcaag gtctctactc ctttcctttc
     3001 caaagtggaa atgggactga ttgaacctac tgaggagttt aaaaaaagtg tatcaaaact
     3061 tctgaaagta gacgcaaggg atctttttat cagcacaaac tgatattaga attcactccg
     3121 atccttcacc tgaagaatcc ggaattatga atataaaaat ctgtttcagg ggaatcagaa
     3181 tctgtccccc ggaacagagt taaattttta agggcaccca ttttcgaaaa ttcagccgat
     3241 gattttcgac agcgtttccc tctgctcctt gtcctccagt gaaagaatga tgttcttcag
     3301 tctttccttc agagccggtt ttccattttc gtatgcatct tcgataatat ctgtctgctt
     3361 tacattgact gaggtaatca gtatgtttct gatcttttta tcttcgtaac tttccatgat
     3421 tgcagaaatt ctttcttttc ggcttacata gttggaaaga agaaggagca tcgagtcgga
     3481 ggtgacatct tttgagattt ccaccacctt gtccagagg
//
LOCUS       DAOQGJ010000163         3508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15287_length_3508_cov_3.713003, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000163 DAOQGJ010000000
VERSION     DAOQGJ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15287_length_3508_cov_3.713003"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..977)
                     /locus_tag="PK683_03695"
     CDS             complement(18..977)
                     /locus_tag="PK683_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HNH07580.1"
                     /translation="MIRTIVTAIFFLIAACKAEPSLDKQKKMNRTLVLGTAAVLYTPF
                     TEVIPSSGEITIKSASGNVSRKYEPACTGTDGNKTFRFLVKKGNSKNLLINFMGGGAC
                     WDSKNCLGDYTPTYFNKLDSSPSYLIKFLFNGILDYSNASNPMNDWNMVFIPYCSGDL
                     HWGSNSLTYKHPSTGASVNFKHKGFDNFLSVLKYIQTNSDFTPGAGNKILVIGQSAGG
                     YGTVFNFSHIKEAFPNSETHAFADASNGVVPAGFQTQAMANWNVTANLPSWVTGISDS
                     FIQTGDLGDMIYKISQYYPSSRFAQYTAEYDGNQRYFYNVSTK"
     gene            complement(1022..1855)
                     /locus_tag="PK683_03700"
     CDS             complement(1022..1855)
                     /locus_tag="PK683_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07581.1"
                     /translation="MSRKKKTGKIRDIGSRYKNFIINKVPLRIHMSLILMCTALTGVL
                     SSKLLFMTGLNPIWLRYLSVVIISYLFFIIYIRLWLAYIGPGDRKSEASSGSLDLTIT
                     DLSIGSSSSAEKTAESGAEISRKGGEFGGGGGTVSWEDGGGSGDAVKAAASVSDSGGS
                     GSGSGSGFDLDIDGDAAGPILVLIVAGALILAVFGGAVYLIYEAPMILSEASFEVFLA
                     AGLLKSTKEFGTGNWIGSVFKKTWIPFTVIAAVSVIAGAVLSSFCPNTLKLMDAIRSC
                     T"
     gene            complement(1902..2429)
                     /locus_tag="PK683_03705"
     CDS             complement(1902..2429)
                     /locus_tag="PK683_03705"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH07582.1"
                     /translation="MISTVPAITMSEMEENPEKWERFEMYKGEAVEMTYTKPAHGRIL
                     LKLGSIILDWIRKNGYGEVYGGESGIKFNDQTRYSFDLGWSDTALPEDEIPTKSLPLM
                     VEIVSDSNDAGLLLTKIEDYLEYGAREVWIVYPKRKSIQVYYPDRTARIFHEQEIIIP
                     GNWMREFQIKPGDLF"
     gene            complement(2475..3074)
                     /locus_tag="PK683_03710"
     CDS             complement(2475..3074)
                     /locus_tag="PK683_03710"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH07583.1"
                     /translation="MAFFSIMGYAAKKEKISPELYLELERKAEFKSELINGEMYAMVG
                     TTRKHNRIAGNLYIGIDRILKSRPCKVYISDLRVQINSTGLFTYPDLIITCGKEILTD
                     KHKDTLTNPKCIIEVLSDSTEKYDRGEKFFNYQQIESLEEYILVSQDQNRVETFLRQT
                     DGKWLYQCTEGLDSKVRINTAEESISLSEIYSNTHEIED"
BASE COUNT          966 a          797 c          699 g         1046 t
ORIGIN      
        1 ccaatcgtgg acgtctgcta ttttgttgaa acgttgtaaa aatatctctg gttcccgtcg
       61 tactctgcag tgtactgtgc gaatctggaa gagggataat actgggatat tttatatatc
      121 atatcaccca gatcgccggt ctgaatgaaa ctgtctgaaa ttcctgtcac ccaggagggc
      181 agatttgcag tgacgttcca atttgccatt gcctgagtct gaaatcccgc cgggacgact
      241 ccgttggaag catctgcaaa tgcgtgggtt tctgaattcg ggaaagcttc ttttatatga
      301 gaaaaattga atacagttcc atatcctccc gcgctctgtc cgatgaccag aattttgttt
      361 cctgcaccgg gagtaaaatc agaatttgtc tgtatgtact tcagaacaga aagaaaatta
      421 tcaaatccct tatgtttgaa atttacagaa gcgcctgtag agggatgttt ataagtcaga
      481 gaatttgatc cccagtgcag atccccggag cagtagggaa tgaataccat attccagtca
      541 ttcatcggat tggatgcatt agaataatct aatattccat tgaacaggaa cttgatcaga
      601 taactcggag aactgtccag tttgttaaaa taagtgggag tataatctcc cagacagttt
      661 ttggagtccc agcaggctcc gccgcccata aaattgatca gaagattctt tgaatttcct
      721 ttttttacca gaaaccggaa tgtcttgttt ccatctgttc ctgtacaggc aggttcatat
      781 tttctgctga catttccaga tgcagatttg attgtaattt ctcctgatga gggaatcact
      841 tcagtgaaag gagtatacag aactgctgca gtgcctaata caagtgtcct gttcattttt
      901 ttctgtttat ccagagaagg ttcggccttg caggcagcga taagaaagaa tattgcagtg
      961 acaattgttc tgatcatacg ataaatagta ctgcagattc ttcttcctgt caagcaggta
     1021 ttcaggtgca ggagcggatt gcatccatca gtttgagagt atttggacag aaactggaaa
     1081 gtacggcccc cgcaatgacg gatacagctg caatcactgt aaaagggatc catgtctttt
     1141 tgaatacact gccgatccag ttgccggttc cgaactcctt agtgcttttt aaaagtcccg
     1201 ccgcaaggaa tacttcaaag gatgcttccg agagtatcat aggagcttca taaatcagat
     1261 acacggctcc gccgaataca gccagaatca gtgcacctgc gacaatcaga acaagaatcg
     1321 gtccggccgc atccccgtca atgtccagat caaatcctga acctgagccg cttcccgatc
     1381 cgcctgaatc cgaaacagaa gctgcagcct ttacggcatc gcccgaacct ccgccgtctt
     1441 cccaggaaac tgtcccgcct ccgcctccaa attcaccgcc ctttctggaa atctctgctc
     1501 cgctctctgc ggtcttttcg gccgatgatg aagaaccgat gctcaggtca gtgatggtaa
     1561 gatccagaga acctgaactt gcctcggatt tcctgtctcc aggaccgata taggcgagcc
     1621 ataagcgtat atatataatg aaaaataaat aagatataat tacaacggaa agatatctca
     1681 gccagatcgg attcagacct gtcataaaaa gaagttttga agagagaacc cctgtgagcg
     1741 ctgtgcacat cagtatgaga ctcatatgga ttctgagcgg aactttattt atgatgaagt
     1801 ttttgtatct tgaaccgatg tcccggattt tgcctgtttt ctttttcctg ctcatattat
     1861 tccttttcga gttcggaact gatcctctgc gaatcccaaa attaaaaaag atctcccggt
     1921 tttatttgaa actcccgcat ccagttccct ggaatgatga tttcctgttc atgaaaaatt
     1981 cttgcggttc tgtcaggata ataaacctga atagattttc ttttaggata gacaatccat
     2041 acttccctcg ctccatactc aagatagtcc tctatctttg tgagcagaag tccggcatca
     2101 ttggaatcag agacaatttc caccatcagc ggaagagact tggtcggaat ctcatcctca
     2161 ggaagggcag tgtcgctcca gcccagatca aatgagtatc tggtttgatc attaaacttt
     2221 atcccggatt cacctccgta aacttcaccg tatccgttct ttctgatcca atctaagatg
     2281 atgcttccca gtttaagcaa aattcttcca tgtgcaggtt tggtatatgt catttcaact
     2341 gcctctccct tgtacatctc aaaccgttcc catttctctg gattttcttc catctctgac
     2401 atcgtaattg cgggcacagt gcttatcata gtctcattat ttttgggaaa aagaaaactg
     2461 tcaacagcaa accctcagtc ttcaatttcg tgagtattgg aataaatctc tgaaagtgaa
     2521 atggactcct ctgcagtatt gattctgact tttgagtcca gcccttccgt acattggtac
     2581 agccattttc cgtcagtctg tctgagaaaa gtttccactc tattctgatc ctgagaaacc
     2641 aaaatatatt cctcaagact ttcaatctgc tgatagttga agaatttttc ccctctgtca
     2701 tatttttctg tggaatcgga cagaacctct atgatgcatt ttggatttgt cagtgtatcc
     2761 ttgtgtttat cagtcagaat ttcttttccg caggtgatga tgagatcagg ataggtgaaa
     2821 agcccggtag aattgatctg taccctcaga tcggaaatgt acaccttgca gggtctgctt
     2881 tttaatatcc tgtcaattcc aatgtacaaa ttcccggcaa tacggttgtg ttttctggtg
     2941 gttcccacca tcgcgtacat ctcaccgttg atcagctcac ttttaaattc agcctttctt
     3001 tccagttcca gataaagctc aggactgatc ttttcttttt tggcagcata acccataata
     3061 gaaaaaaatg ccaaatccaa tccgctgtca agtgattccc gtttcctatt tctccgccta
     3121 ggggacagca gaaaatagtg attatcaccc ccgcctcgac ctctctgagc tgagtccata
     3181 taaaaaaacc taaaaagact gagtttttca ctttgtgatc gcggtgaagc tgtcttcttc
     3241 gaaataggct gtacataacc ccggccttga ttttgggaga ttttttcaaa aaccggacag
     3301 gaaaacttga ataaaaaatt tatattcaaa gggtttttta aataaaatga ttgtaaatta
     3361 gcagggtttc catttcttat actacataat catcatgtca agaattttgg aaaaatatgc
     3421 caataccgat gaggcagtcc tttggaaaga ataccgttct tccaaaaatg cggaaattcg
     3481 tacctacctg gcagaaaaat atgcaccc
//
LOCUS       DAOQGJ010000164         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15394_length_3492_cov_3.019494, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000164 DAOQGJ010000000
VERSION     DAOQGJ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15394_length_3492_cov_3.019494"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PK683_03715"
     CDS             <1..915
                     /locus_tag="PK683_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNH07584.1"
                     /translation="KDEKGNPVQAIYQPIWGIWDSTFCLDVTHPAALDYLEKVFSTFS
                     KEYGYNYLKLDFLYAGALDGVTYNSRISPAQRYSQAIQMIRNTVGKNTFLLGCGAPMF
                     PSAGVFDGMRVSCDVTPFWGVQRLRQWLSDRHALCTEKALINGLYRSFMHRNFWLNDP
                     DCLIIRKDKNKMTYDQTILMASVMGLSGGMLLISDNLSTLDTERMPLFHKAVAISRAC
                     QAESSKPLGLMEYKFPRGIYNPGGAAGFWNPTEETAEISVYYPYPLFWENKPEFWTSA
                     TFRDYEYDKVSRTLKLRLGPFQSVVFIK"
     gene            915..2681
                     /locus_tag="PK683_03720"
     CDS             915..2681
                     /locus_tag="PK683_03720"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH07585.1"
                     /translation="MTDQENYSLFHYSLEKDRLWEEFQARLISEFGIRTFGLIEIDEL
                     NEILWINKYFSDERVSPIFRQIGCDIHEEDHPFNVCFRERKPVRVNLTSAKENFRPFA
                     WSYMKILQKKFLYLFPLVNREKCYSILIFDFSFQEKRDHLLKNKGESLHRLLLIFSKA
                     LYISREYSRYLTKYTQFKNFHTTGLTLSKMYLNNTQEIIRMSLLTLSGIIDANVFYLI
                     VYNEKYRAMTVHALIKTLDRIDIHQSSFEVEDLEEISYLFVTLKPYFIDHNRLKFPRS
                     MGFTGSKIMMLPSFSLESSRYAFALGRNSYKNFSADEIDVLDSYSELAKITIDNSYLY
                     HKMTRQARLENEVEIARDIQMNLIPRTAPVSADYEFAGFMIPAREIGGDYYDFLQSPD
                     GIDTISAIGDVSGKGIPAGMVMATAGTILHSVVRKKVSLRDMLFELNSHLYHSYKNSN
                     VQRFMSLTLLKFYHGKNKVEYAGAGHGHILIYREKTNKVEVVPTGGMILGIVPEGTVE
                     SGSFRMEKGDILFLFTDGATESQDTKSEFYGESRLLTSFQNAVKKPPREILVKVYEDI
                     IRFTNNHMQQDDITLLCVKKNA"
     gene            2674..>3492
                     /gene="tilS"
                     /locus_tag="PK683_03725"
     CDS             2674..>3492
                     /gene="tilS"
                     /locus_tag="PK683_03725"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNH07586.1"
                     /translation="MHETSERIFQSLAERSKNFFELFRKKTLISFSGGKDSRILLELY
                     RLLHRNGITPEPVIFHLNHRIRDNSEQEKEILEFIKNTGYPFIYREENIPAVASEKKM
                     SLEEAGRFVRQDLLKKAAEKENLIIALGHHSDDYLESVLIHLIRGAGEAAFRTMSVFE
                     DRIFRPLLFFKMKEMQEILKTEDWKIFEDESNASMKYTRNRIRHGVMPLLSEEGADSF
                     KTYWNFHDSPDEALADTAPPPKTNAVQRISGHRISEAGLSGLKRILDSYSRLLGG"
BASE COUNT         1069 a          758 c          772 g          893 t
ORIGIN      
        1 aaagatgaaa aaggaaatcc tgttcaggca atttatcagc cgatctgggg aatctgggac
       61 agcactttct gcctggatgt cactcatcct gctgcactcg actatctgga aaaagtattc
      121 agtacattct ccaaggaata cggatacaat tatctgaagc tggacttcct ctatgcgggg
      181 gctctcgacg gtgtcaccta caacagcaga atctctcctg cacagagata ctcgcaggca
      241 atccagatga tcagaaatac agtcggcaaa aatactttcc ttctgggatg cggagcacct
      301 atgtttcctt ctgcaggagt atttgacgga atgagagtga gctgtgatgt gactcctttc
      361 tggggagtgc agagactgcg ccagtggctt tcagacagac atgcactctg tactgaaaag
      421 gctctcatca acggtctgta cagaagtttc atgcacagaa atttctggct gaacgaccct
      481 gactgtctga ttatcaggaa agataaaaat aagatgacct acgatcagac gatactgatg
      541 gcttcagtca tgggtctctc gggaggaatg cttctcatca gcgacaatct gtccacactg
      601 gatacggaaa gaatgcctct tttccacaag gctgttgcca tcagcagggc ttgtcaggcg
      661 gaatcttcga agccgcttgg actcatggaa tacaaatttc cgagagggat ctacaatccg
      721 ggaggagcag caggcttctg gaatccgacc gaagagacag ctgaaatctc agtgtactat
      781 ccttatcctc ttttctggga aaacaaaccc gaattctgga catccgcaac attcagagac
      841 tacgagtatg acaaggtatc caggacactt aagctcagac tcgggccgtt tcagtcggtc
      901 gtgtttataa aataatgaca gatcaggaaa attacagtct ttttcattac agcctggaga
      961 aagacagact ctgggaggaa tttcaggcca gactcatatc cgaattcgga atcaggacat
     1021 tcggcctgat cgaaatcgat gagctgaatg aaattctctg gatcaacaag tatttttccg
     1081 acgaaagagt ttctcctata ttccgtcaga tagggtgtga tatccatgag gaagatcatc
     1141 ctttcaatgt atgcttcaga gagagaaagc ctgtccgggt aaacctgacc tctgcaaagg
     1201 aaaattttcg tccatttgcc tggtcgtaca tgaaaatcct gcagaaaaaa tttctgtatc
     1261 tttttccgct ggtgaaccgg gaaaaatgct attccatcct gatctttgat ttttccttcc
     1321 aggaaaaaag agaccatctt ctgaaaaata aaggagagtc tcttcacaga ctgcttctca
     1381 ttttttccaa ggcactctac atttcgagag agtacagcag atacctcact aagtacactc
     1441 agttcaagaa tttccatacc acaggtctga ccctgagcaa gatgtatctg aacaacactc
     1501 aggaaatcat caggatgtct cttctgaccc tttcaggaat cattgatgca aatgtttttt
     1561 atctcattgt atataatgaa aaatacaggg caatgacagt ccacgcactg atcaagacac
     1621 tggacaggat agatattcat cagtccagtt ttgaagtgga ggacctggag gagatcagtt
     1681 atctgtttgt aacactgaag ccttatttca ttgatcataa cagacttaaa tttcccagat
     1741 ccatgggatt caccggatca aagataatga tgctcccttc cttttcactg gaatcgagca
     1801 gatacgcatt tgcactggga agaaactcat acaaaaattt cagtgcagat gaaattgatg
     1861 tcctcgattc ctattctgaa cttgcaaaga tcaccatcga caactcctac ctctatcata
     1921 aaatgacaag gcaggcaaga ctcgaaaatg aagtggagat agcccgcgac attcagatga
     1981 atctgattcc tcggactgct cctgtttctg cagactatga gtttgccgga tttatgattc
     2041 ctgcaaggga aatcggagga gattattatg atttcctgca gtctcccgac ggcatagata
     2101 caatctcagc aatcggggat gtatccggaa aaggaatccc tgcaggaatg gtgatggcaa
     2161 ctgccggaac aatcctgcat tcagtcgtca gaaaaaaagt ttctctcagg gatatgcttt
     2221 ttgaactgaa ctcacatctc tatcacagtt ataaaaattc caatgtccag agattcatgt
     2281 cgctcactct tctgaagttt tatcatggaa aaaacaaggt ggaatatgca ggagcaggtc
     2341 acggtcatat tctgatctac cgcgagaaga caaacaaagt ggaagtcgtg ccgacaggcg
     2401 gaatgatcct gggaattgtt cctgaaggaa ctgtggaaag cggctcattc aggatggaaa
     2461 agggagacat tctttttctt ttcaccgacg gagctactga atctcaggat actaaatctg
     2521 aattctacgg tgaaagcagg cttcttacaa gctttcagaa tgcagtgaaa aaacctccga
     2581 gagaaattct ggtgaaagta tatgaagata ttatccggtt cacaaacaat catatgcagc
     2641 aggacgacat cactcttctc tgcgtgaaaa aaaatgcatg aaacttccga aaggatcttt
     2701 cagtctctcg cagaaagatc aaaaaacttc ttcgaactct tcagaaaaaa aacactgatc
     2761 tcattttcag gcggaaagga ctccagaatc cttctggagc tgtacagact tcttcacaga
     2821 aacggcataa cgcctgaacc tgtgattttt catctgaacc acagaatcag agacaattca
     2881 gaacaggaaa aagaaatact ggaattcatt aaaaatacag gatatccgtt tatataccgg
     2941 gaggaaaata tccctgcagt cgcttcggaa aaaaaaatga gtctggaaga agcaggaaga
     3001 ttcgtcaggc aggatcttct gaagaaggca gccgaaaaag aaaatctgat catcgctctg
     3061 ggacatcact ctgacgatta tctggaatct gttctgattc atctgattcg cggtgcaggc
     3121 gaggctgcat tccgcacaat gtctgtattt gaggacagaa ttttccgccc tctccttttt
     3181 ttcaaaatga aagaaatgca ggaaatactg aaaactgaag actggaaaat ctttgaagat
     3241 gaatccaatg cttctatgaa gtacactagg aacaggatca ggcatggagt aatgcctctt
     3301 ctttcagagg aaggagcaga cagcttcaag acatactgga actttcacga cagtccggat
     3361 gaggcgttgg ccgatacagc accgccgcca aagacgaatg cagtgcagag aatttcagga
     3421 cacaggattt ctgaagcagg tctttccggt ctgaaacgaa ttctggattc ctattcgagg
     3481 cttctgggcg ga
//
LOCUS       DAOQGJ010000165         3488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15425_length_3488_cov_3.409263, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000165 DAOQGJ010000000
VERSION     DAOQGJ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15425_length_3488_cov_3.409263"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            704..1228
                     /locus_tag="PK683_03730"
     CDS             704..1228
                     /locus_tag="PK683_03730"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HNH07587.1"
                     /translation="MEDIDIQLYQEAIQKNISHSGSGVSTVLIPEDLMLRYETQEQKF
                     GGKTQYFSHLISKYRMMLRSFAHDESSMTTEYQKSSHKLKKVNFRPIAEDWAELGAFS
                     IASGKSRCLLFVLLLLMDLNGWGDLLSLSGFPYDYPNTPNIQWELLGIFGVERAVSKF
                     IRGLMSRLIRMENN"
     gene            1462..3429
                     /locus_tag="PK683_03735"
     CDS             1462..3429
                     /locus_tag="PK683_03735"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07588.1"
                     /translation="MNLEKWDFVQFGNLELNGQWEFYYQQFLSSEDIAEGRGHLTGYI
                     NFPGTWRNFQAGDSKISGHGYGTFRLRIRLPNHPHISMKTATVGTSYKMFLNGKEVLG
                     GGQIGQTEEKTVPGFHFTVLPVYPDSRSEAEIILQIANFSDPNGGLWESMTVGDTASV
                     LISRKFQFMMDIFLFSTLMVMGIYHITLFLLRRKDLSPLYFGIFCMMIGLRTLLTSER
                     ILHNDVAIFSWDFLFSAEYLTYYVSVPLFSGFIYSLYKEEFSLRIAQVIFGVSTVFIL
                     LVIFTKVKFFTEINVIFQIFTLLTVVYMFFALLKALFRKREDIIFFFLGMLFIVFSVV
                     HDILVASHILHTVYIFGYGLAAFILFQSTIIASRFSRAFLKSEELTESLIITNYSIAR
                     FVPSEFLRLLNRNNIVDVRLGDHLKQEMTVFFSDIRGFTSLAEKLNSEDVFKFLNTYF
                     SQTISIISRNHGFVDKIIGDGIMAIFPSSPNDAVQASMEIMEAISEWNRLRKKEGLLP
                     VTAGIGIHTGTVSMGTVGTEDRLDTTVIGDTVNIASRLENLNKDYRTKVILSETVYSG
                     LDEKWMKYIRELDNTQIRGREKNLTIFECFAGADTDTVSKIMKAGSLYKEALLCFKQK
                     NFTEAKRLFSKCRDIHPEDSVVQQYIQRCSV"
BASE COUNT          998 a          671 c          756 g         1063 t
ORIGIN      
        1 atatccttat tttttccttc aaaacagaaa tagaaacaat tgaggaatag tctcactgtt
       61 gacggctctg acgctgagag aatgtcggct gtcctctttc caggccgaag tatacttata
      121 ctcattttca aaagtcattg cgcattcatt ccttatcgac tcaatagcat taaggtagcc
      181 agtaagaaaa gacgcaatta tttatgaata tcagtatgta gggaaagcca ttttccagaa
      241 ggcagtccga ctgccgttga ataagtgcca ttatcaaagc ttacttctat agaggtaaca
      301 gttcctgaag ctgtgccaat cagaaatcca gtctgaaaaa ttgaattgtt tatcagattg
      361 gcaataactg gagggctgac cgtaaaagtt gatgtctgac tgcatgtgat tgaagaagaa
      421 agacgaaata tagcgtatgc atttgtccat tcaagaaaac ttttcttaat gctggtattg
      481 cagttcaaga taagcagaat cagcagtgtc cgtaagcaca tgtaccgaaa tttacagtac
      541 cgtttaaatc agtcaacttt ttttactaaa tttcttgaat caggagcagg aattcttctt
      601 gccttctctt ctgtgaaata cctctttgaa gaaaaattca ctctatgatg aaataattaa
      661 aaaaaatctg ctcaattgaa taaaaaatat acaggtaagt aatatggaag atatcgatat
      721 acaactttac caagaagcaa tacaaaagaa tatttcacat tccggaagcg gagtttccac
      781 tgttctcatt cctgaagatc tgatgctccg ctatgaaact caggagcaga agtttggagg
      841 aaagactcaa tatttctctc atctgatttc caagtatcga atgatgctca ggtcgtttgc
      901 tcacgatgaa tccagtatga ctactgagta tcagaaaagc agtcacaaat tgaagaaggt
      961 caatttccgt cctatagcgg aggactgggc tgagcttggt gctttttcta tcgcatccgg
     1021 taaaagcagg tgtctgctgt ttgttctcct tcttctcatg gatctgaacg gctggggtga
     1081 tctcctcagc ctttccggct ttccctatga ttaccccaac accccgaaca tacagtggga
     1141 actcctaggg atcttcgggg tggagagggc agtcagtaaa tttatcagag ggctgatgtc
     1201 gaggctgata cggatggaaa ataattaaaa aaaatgatac attcctaaag cggaaacgcc
     1261 tgccgccgga tcggttccac cgtcattttt gaaacaaatt ctattctaaa aattgcttgc
     1321 tgaaattgca gtggaataat attgacttgg agcatgggtt ttatcgttcg gggatcggtt
     1381 ctgtttttaa ttttttggat gttctttctg ttttcttcct gcagcagaaa gtctgccgtt
     1441 ccttctgctg tacaaggttt gatgaatctg gaaaaatggg actttgttca gttcggaaat
     1501 cttgaactga acggtcagtg ggagttttat tatcagcagt ttttatcttc ggaggatatt
     1561 gctgagggca gggggcattt gaccgggtat atcaattttc cgggaacttg gagaaatttt
     1621 caagcgggcg attcaaaaat ttccggccac ggttatggaa ctttccgtct cagaatccgc
     1681 cttccaaatc accctcatat atcaatgaag acggcgactg taggaacttc ctataaaatg
     1741 tttctgaacg ggaaagaagt tttaggaggg gggcagatcg gtcagacgga agagaaaact
     1801 gttccgggtt ttcactttac agtgctgcct gtctatccgg acagcagatc cgaggcggag
     1861 atcatactgc agatagcaaa tttttccgat ccgaacggtg gcctctggga aagcatgact
     1921 gtcggagaca cagcgtccgt gctcatttcc aggaaatttc agttcatgat ggatatattt
     1981 cttttcagca cgctcatggt aatggggata tatcatatca cactttttct gctgagaaga
     2041 aaggatcttt ctccgcttta cttcggaatc ttctgcatga tgatcgggct cagaactctt
     2101 cttacttcag aaaggatact tcataatgat gttgcaatat tcagctggga tttccttttc
     2161 tctgcggaat acctgactta ttatgtgagt gttcctcttt tttcagggtt tatatacagt
     2221 ttatacaaag aggaattttc cttaagaatt gctcaggtca ttttcggtgt cagtactgta
     2281 ttcattctgc ttgtcatttt cacgaaagta aaatttttta ctgaaataaa tgttattttt
     2341 cagatattta ctctgcttac agtagtgtat atgttttttg cacttctcaa ggcacttttc
     2401 agaaagaggg aagacatcat attctttttc ctggggatgc tgttcattgt gttttccgtt
     2461 gtgcacgata tactggttgc atctcatatt cttcatacgg tttatatttt cggttatgga
     2521 cttgccgcct tcattctgtt tcagtcaaca atcattgcct cccgtttttc aagagcattc
     2581 ctgaagtctg aggaacttac tgaaagtctc atcattacaa attattccat tgccagattt
     2641 gttccttctg agttcctgcg ccttctgaac aggaacaata ttgtcgatgt caggcttgga
     2701 gatcatctga agcaggagat gactgtattc ttttcagaca tacgcgggtt taccagcctt
     2761 gccgaaaagc tgaattcaga ggatgtgttc aagtttttga atacatattt cagtcagaca
     2821 atttccatca tcagcagaaa tcacgggttt gtggataaga tcatcggtga cggaatcatg
     2881 gcgatttttc caagttcacc aaacgatgca gttcaggctt ctatggagat catggaggca
     2941 atttcagaat ggaacaggct tcgtaaaaag gagggacttc tgcctgttac tgcgggaatt
     3001 ggaattcata ccgggacagt cagtatggga actgtaggca ctgaagaccg gctggataca
     3061 actgtcatcg gtgatacagt caacatagcc tcccgactgg aaaatctgaa caaggactac
     3121 agaaccaaag tgattctttc agagacggtc tattccggtc tggatgaaaa atggatgaaa
     3181 tacatcagag aattggacaa tacacagatt cgcggaaggg aaaaaaatct cacaattttc
     3241 gagtgttttg caggagcaga tacagacact gtttccaaaa taatgaaagc aggttcctta
     3301 tataaagaag cactgctatg ctttaagcag aaaaatttta cagaagcaaa aagactgttc
     3361 agtaagtgca gagatattca tccggaagat tctgtcgttc agcagtatat tcaaaggtgt
     3421 tccgtctgac aggcgtgcaa aggcttgagc atcagataaa atgcactttt cagccattat
     3481 cagtctgt
//
LOCUS       DAOQGJ010000166         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15434_length_3487_cov_2.792541, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000166 DAOQGJ010000000
VERSION     DAOQGJ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15434_length_3487_cov_2.792541"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK683_03740"
     CDS             complement(<1..341)
                     /locus_tag="PK683_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNH07589.1"
                     /translation="MKEVVDEYLKEPGRLPSMSQVVLKVMNLVQDPSVSMQLLAAEIS
                     KDPAITAAIIKISNSAYYRASRPIRTVHEALMTLGIQTVKEIVLISASKGILHKDLKG
                     YQLEAEDVWVHS"
     gene            complement(351..857)
                     /locus_tag="PK683_03745"
     CDS             complement(351..857)
                     /locus_tag="PK683_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNH07590.1"
                     /translation="MLGGKDKVINVGIAEIQVARNPGILRTTLGSCIGIVFYQPELKI
                     GAISHIMLAKDVIGKDKAKNPGKYGETALPKLIQQFEEEGCRPGTYSARIFGGASMFK
                     NISSPFLQNIGENNIQIVREILSSRKIPIIAEDVGGHDGRTISLFLDDGRILMKKAGV
                     EKFIYKVK"
     gene            1027..1578
                     /locus_tag="PK683_03750"
     CDS             1027..1578
                     /locus_tag="PK683_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07591.1"
                     /translation="MILPLFIVLLLSPIFHANADDEFIFDRSSFPAKFKSGILSTGID
                     CEHIKLMRNGNFEYKFTGDCKGWGRKLKGKWVKKEDHISLSGFLKENQEEGETGCAGS
                     YYHTNTPEEKKECFQRYKNKILERFGKFPAIFEMKGKIWITEESTVGVSIETYMLKSD
                     KKNTKGLMFFSDEKFGAFGKLED"
     gene            1653..1814
                     /gene="rpmG"
                     /locus_tag="PK683_03755"
     CDS             1653..1814
                     /gene="rpmG"
                     /locus_tag="PK683_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNH07592.1"
                     /translation="MREIIKLACTGCAQPGKSTYFMTKNKKAKSEKLVTKKFCKFCRK
                     HQDHKESKV"
     gene            1814..2185
                     /locus_tag="PK683_03760"
                     /pseudo
     CDS             1814..2185
                     /locus_tag="PK683_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
     gene            complement(2273..>3487)
                     /locus_tag="PK683_03765"
     CDS             complement(2273..>3487)
                     /locus_tag="PK683_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07593.1"
                     /translation="PVSEYQPEQTLSEEQNRAVKTALESRLTLLTGGPGTGKTSILKV
                     VLKKLFQSGISPKQTALTAPSGKAAKRMQESMEDLFREYPDLSAPVTIHRLLGYNPAS
                     SAFRFNSENPINASVLIADEASMIDVRIMNALLKAFCFNGIENSRLILSGDPNQLLSV
                     DSGQVFSDLVQSGINTVRLTKTFRQSEEAASIRIFSENILKGTAKLQDLQRMEEGEDF
                     TGIRMADSDETDPEKILRWYADRQSESLQILSPYNEGTGGVDEINRFMQEKMDSENSC
                     PAMVTRNLSDLNLFNGETGLLSLNGGELFFIQSGKKTKIPYYLKPCIVPAYAVTIHKS
                     QGSEYDHVCLVLPERSRQGKMNSVLNIRLLFTAATRAKKSLLIIGKEEVFKEAMQNIP
                     KRKSALLDKLNS"
BASE COUNT         1029 a          746 c          707 g         1005 t
ORIGIN      
        1 gagtgcaccc atacatcttc tgcttccagc tgatatcctt tcagatcctt atgaagtatt
       61 cccttggatg cactgatcag cacaatctcc ttgactgtct gaatgccgag agtcatcaaa
      121 gcttcatgca ctgtccgaat cggtctgctt gctctgtaat atgcagaatt ggatatttta
      181 ataattgctg ctgtaattgc agggtctttg gagatttcag cggcgaggag ctgcattgaa
      241 acagatggat cctgcactag attcatgact ttcagaacta cctgcgacat agagggaagt
      301 cttccaggtt ctttcaaata ttcatctaca acttctttca tccgttacct ctattttacc
      361 ttataaatga atttttcgac gcctgctttt ttcataagaa tccttccgtc atccaaaaac
      421 agactgattg ttctcccatc atggccgccg acatcttctg caataatcgg gatctttctc
      481 gaagaaagaa tctctctgac gatctgaata ttattctctc ctatgttctg cagaaaggga
      541 gaagatatat ttttaaacat ggaagctccg ccgaatattc tggctgagta tgttcccggc
      601 ctgcatcctt cttcttcaaa ctgctgtata agtttcggaa gcgccgtctc tccgtatttt
      661 cctggatttt tagccttgtc tttgccgatg acatctttgg ccagcataat atgtgagatt
      721 gctccaattt tcaattcagg ctggtaaaaa acaatgccta tacatgaccc taaagtagtc
      781 ctaagaatcc caggattcct tgccacttga atttcagcaa ttcctacatt aatgacttta
      841 tcttttccac ctaacatatg gcttgaatta ggttaatttt atgagaatat cagtaaagca
      901 aaaaaccgaa gatttactct gatttttctc agaaagagcc agggaagatt acagcagccc
      961 tgcctgaaaa ataaaatgat tgcgaatcat cagcagtagt aatccttaga gaaaggtcta
     1021 tgcattatga ttctgccttt attcattgta ctgctgctgt cccctatctt ccatgcaaat
     1081 gcggatgatg aatttatctt tgaccggtca tccttcccgg caaaattcaa atccggcatc
     1141 ctttcaacag gaattgactg tgaacacatt aaactgatgc ggaacgggaa ctttgaatac
     1201 aaattcaccg gtgactgcaa gggctggggc cgaaaactga aaggcaagtg ggtaaaaaag
     1261 gaagatcata tttccctgtc aggttttctg aaggaaaacc aggaagaagg agaaacaggc
     1321 tgtgcaggct cctactacca cacaaataca ccggaagaga agaaagaatg ctttcagcgc
     1381 tataagaata aaattcttga aagattcggg aaatttcctg ccatatttga aatgaaggga
     1441 aaaatatgga ttacagagga atccacagtc ggcgtaagca ttgaaactta tatgcttaaa
     1501 tctgataaaa agaatacaaa gggtctgatg ttcttttcgg atgaaaagtt cggggcattc
     1561 gggaaactgg aagattgaga tcggcaattt gcagaaaaaa aggaaaacaa aaaaattgac
     1621 ggtacaatga agtaattttt tatggctcta ttatgagaga aattataaaa cttgcttgta
     1681 ctggatgtgc ccaaccaggt aaatctactt atttcatgac taagaacaaa aaagcaaaat
     1741 cagaaaaact ggttaccaag aaattttgca aattttgcag aaaacaccaa gatcataaag
     1801 agtctaaagt ctgatggcag cgaaaactaa atcaggtctg gatacaaaat tcataaatga
     1861 gatcaagaaa tctcttttag aaaagagaca gtctctaatg gagaaattga acacttggga
     1921 ggacaaaagt tcgccctcag gcctgaagga aatgggagat atcgctgata tcgcttctga
     1981 aatcaacgaa gaagctctca attccgcttt gactgaaaac gagattgatc ttctcagact
     2041 cattgaaaaa gctttggtaa aaatcgataa tggtacttac ggaatctgcg aaggcagcaa
     2101 gaaaaaaatt ccggttgcca gacttcgtgc aatcccttgg acaccttata cagtggaata
     2161 cgcggaaatt gctgcaaaaa acaaagcaaa gcagattcat gagagaagag tctctgaaag
     2221 ccagccatat gtgcaggctc tcgatatcag ttacggagac gactgaacca gtctaactgt
     2281 tcagcttatc cagcagtgca gatttccttt taggaatatt ctgcattgct tctttaaata
     2341 cttcttcctt ccctattatc aaaagactct ttttagccct ggtcgctgcc gtaaacagaa
     2401 gccttatatt cagaacagaa ttcatttttc cctgcctgct tctttccggc agaacaagac
     2461 acacatgatc atattcagat ccctgacttt tgtgaatagt cactgcatag gcaggcacga
     2521 tgcaaggctt cagataataa ggaatcttag tttttttgcc ggactgaata aaaaacagct
     2581 ctcccccatt caatgaaagg agtccggttt ctccgttgaa cagattcaga tcagacaggt
     2641 tccgggtcac cattgccgga cagctgtttt ctgaatccat tttttcctgc ataaaccggt
     2701 tgatctcatc cacgcctccg gtcccctcat tgtacggact cagaatctgt aaggattcag
     2761 actgtctgtc tgcataccaa cgcagtattt tttccggatc ggtttcatct gaatcagcca
     2821 tccgtatccc tgtgaaatcc tccccttctt ccattctctg cagatcctgc agtttcgctg
     2881 ttcctttgag tatattttca gaaaaaatcc tgatggatgc ggcctcttca gactggcgga
     2941 aagtttttgt gagtctgact gtgtttattc cagactgaac aaggtcagaa aatacctgtc
     3001 cggaatccac tgaaagaagc tgattcggat cacctgacag aatgagcctg gaattttcaa
     3061 tgccgttgaa gcagaatgct ttcagaagtg cattcataat cctcacatca atcatggaag
     3121 cctcatctgc aatcagtacc gaagcattaa ttgggttttc tgaattgaat cgaaaagctg
     3181 aactggcagg gttatatcct agaagcctgt gaattgtgac cggtgctgaa agatcagggt
     3241 actctctgaa aagatcttcc atactttcct gcatccgttt agcagccttt ccggaaggtg
     3301 cagtcagagc ggtctgtttt ggactgattc ccgactgaaa aagttttttc agaaccacct
     3361 tcagaatgga tgtttttcct gtgccgggtc ctcctgtaag aagcgtaagt cttgattcaa
     3421 gggctgtttt tactgccctg ttctgttctt cgctgagggt ctgttccggc tggtattccg
     3481 atactgg
//
LOCUS       DAOQGJ010000167         3476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15510_length_3476_cov_2.710903, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000167 DAOQGJ010000000
VERSION     DAOQGJ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15510_length_3476_cov_2.710903"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PK683_03770"
     CDS             <1..1105
                     /locus_tag="PK683_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA phosphorothioation-associated putative
                     methyltransferase"
                     /protein_id="HNH07594.1"
                     /translation="AEDLLAVSVMIRDRGILPGEKEYSDGIVTSWNTFQKYYTQKSFK
                     EYLQENLKAEIHSAGIGIFYIFKNSDWVEKYLNARMQKRPASVSVQIRQEEHSRILAD
                     WLDLYQELGRIPVQAEFKEYKYVLKYFGSMENAAESMRAEIDQDTFFENSRRRKEKLN
                     VALCRHFIRKNGAPKWKDLRTEEQTDIKIYYSDFNEALNFAMSELKTLSDLSTVSRYI
                     SESSVGKVLPDDIYIHSDSLRYAPEMLQILVELAKIILPPDFEYNIIKIARNSWHVTF
                     LNYPEFYNDPHPALKDSIKVFLHKNQIGYRDYSSSENPPILHRKETFLHSSHPKYEEY
                     SALTGAEESAGLLSRRDIGTRKGWEKVLKEKGL"
     gene            1381..2982
                     /locus_tag="PK683_03775"
     CDS             1381..2982
                     /locus_tag="PK683_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNH07595.1"
                     /translation="MFDYIIIGGGSAGCLLANRLSENPDNQVCLLEAGPPDSSSFIHN
                     CNPVNMLYLMNSRKYNWRYQAEGNEKTGKRRFFWPRGRTLGGSSAVNAMIYTRGHKSD
                     FDHWAELGCKGWEYESVLPYFMKSELQHRGEDAFHGTKGTMDVIDPTFHFPASEAFVE
                     ACVQSGIPRNRDMNGSEYEGVDFFQINQTPKGRRCHSSVSFLDPVRGRKNLTVITDAH
                     VRRILLKDKTAYGIEYYDLSDRRKLKVLETRKEIILSAGIIGSPQILKLSGIGPAPEL
                     KKFGIEIIADLPGVGENLQDHPDIIIRCRDKSGTSLTVFPWPYMVRFLSSFYQSKKPF
                     IFTPTDAGGFIKSDPSVNVPDLQLQFASLRMQPHGRGLFTPALSGFVLHICHMRPKSR
                     GRVLLNSADPFIPPRIEANYFADSFELEALVNGVKIARKILSQPALEPFFGKEEIPGA
                     AVQSDEEIRSFILQKAESAYHTAGSCKMGIDSLSVVDPALKVHGIQNLRVIDSSVMPT
                     ITGSNIHSPTVMIAEKGAELILKGN"
     gene            complement(2992..>3476)
                     /locus_tag="PK683_03780"
     CDS             complement(2992..>3476)
                     /locus_tag="PK683_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNH07596.1"
                     /translation="YLDALEGTFLFSFLPPYFSNLRKEISKMPKVYTEDFGIVKYVMK
                     TPESNSYDILSGQDVETYCFRIFRKIFEMENLFFHRTVSKSEIDFIGRKNSSFIPIEI
                     KFTNRVQGKITLAMKNFSERYLDIPYFIFITKDTLAWNKENRIFYIPVYLLEFMDILS
                     "
BASE COUNT         1040 a          687 c          809 g          940 t
ORIGIN      
        1 tgcagaagac cttctggctg tgtccgtgat gatccgcgac aggggaattc tgcccgggga
       61 aaaagagtat tctgacggga tagtcacatc ctggaacact tttcaaaaat actatactca
      121 gaaaagcttc aaggagtatc ttcaggagaa tctgaaagcg gaaatccatt cggcaggaat
      181 cgggatattt tatatattca aaaactctga ctgggtggaa aagtatctga atgctaggat
      241 gcagaaaaga ccagcttctg tctcagtgca gatcaggcag gaggagcaca gcagaattct
      301 tgcagactgg ctggatctgt atcaagagct tggccgaatt ccggttcagg cagagttcaa
      361 ggagtataag tatgttctga aatacttcgg aagtatggag aatgctgcag aatcaatgcg
      421 tgcagaaatt gatcaggata cattctttga gaattccaga agaagaaagg agaagctgaa
      481 cgtcgctctc tgcagacact tcatcaggaa aaacggtgct ccgaaatgga aggatctcag
      541 gacagaggaa cagacagaca taaagattta ttattcggat ttcaatgagg cactgaactt
      601 tgcaatgagt gagctgaaga cactgtctga tctttctaca gtgagcagat atatttccga
      661 atcttcagta ggaaaggtgc ttcctgacga tatttatatc cattccgact ctctccgcta
      721 tgcaccggaa atgctccaga ttctggtaga gcttgcaaaa atcattcttc cgccggattt
      781 tgaatataat ataattaaaa ttgcaagaaa ttcatggcat gtcaccttcc tgaattatcc
      841 ggaattttat aatgatcctc atcctgcact gaaagattcc attaaagttt ttctgcataa
      901 gaatcagatc ggatacagag actacagcag ttcagaaaat ccgcctattc ttcacaggaa
      961 ggagacattt cttcattcct ctcatccgaa atatgaagag tacagtgcac tgactggcgc
     1021 agaggagtct gccggacttc tttccagacg ggatataggc acaagaaagg gatgggagaa
     1081 ggtgctgaag gaaaaggggt tataaatatt ttagattcag cattgttgaa cactgtcttc
     1141 agcagtcaga cagccgaaga cagtaatctg tgttatgggc gtttaggtaa agttccgctg
     1201 attgaaacac agtagcgcat ttttgcactg catcaaattg tctataaaat gttttctccg
     1261 tgatatcggt tctgaaataa tccggatgaa caggagattt actgtttcag caggtgaaaa
     1321 cagacagaat tgcagaaata aagaaatgga ctttgaaagg ccgtgataat ccatataatt
     1381 atgtttgatt atatcattat aggaggggga tctgcaggat gccttctcgc gaaccgcctc
     1441 tctgaaaatc cggacaatca ggtctgtctt ctggaggccg gtcctccaga cagttccagc
     1501 ttcatccaca actgcaatcc agtgaatatg ctctatctga tgaacagcag aaaatacaac
     1561 tggcgttatc aggctgaggg taatgaaaaa acaggcaaac gccgattttt ctggccgaga
     1621 ggaagaacac tcggcggttc cagtgcagtc aatgcaatga tatatacaag gggtcataaa
     1681 tctgattttg atcactgggc tgaacttgga tgcaagggct gggagtatga atcagtgctt
     1741 ccgtacttta tgaaatcgga actgcagcac aggggagagg atgcatttca cggaacgaaa
     1801 ggcacaatgg atgtcattga tccgaccttc cattttcctg caagcgaggc ttttgtcgag
     1861 gcctgtgttc agtcagggat tcccagaaac cgtgatatga acggttctga atatgaggga
     1921 gtggattttt ttcagatcaa tcagactccg aagggaagac gctgtcattc ttcagtctca
     1981 tttctcgatc ctgtccgggg caggaaaaat ctgactgtaa ttacagacgc ccatgtgcgc
     2041 cggatccttc tgaaagataa aactgcttat ggtatagaat attatgattt aagcgacaga
     2101 aggaaactga aagttctcga aacacgaaaa gaaattatat taagtgcagg gataataggt
     2161 tcacctcaga ttctgaagct ttccgggata ggtcctgcac ctgagctgaa aaagttcggg
     2221 atagaaatca ttgcagatct gccaggagtg ggagagaatc tgcaagatca tcctgacatc
     2281 attatccgat gcagagacaa gtcaggcact tcactcactg tgtttccctg gccgtatatg
     2341 gttcgttttc tgtcgagctt ctatcagtca aaaaaacctt tcatattcac gcctactgat
     2401 gcaggagggt tcattaaatc agatccgtca gtcaatgttc ctgatcttca gctccagttc
     2461 gcatctctca gaatgcagcc tcacggaaga ggtctgttta ctcctgcact gtccgggttt
     2521 gttctgcata tctgtcatat gaggccgaag agcagaggaa gggttctgct gaattctgct
     2581 gacccgttca tccctccgcg aatagaggca aattattttg cagacagttt cgagcttgag
     2641 gctcttgtga acggagtaaa aatcgcgcgt aaaattcttt cacagcctgc acttgagccg
     2701 ttcttcggaa aggaagagat tccgggagca gctgttcagt cggatgagga gatccgaagt
     2761 tttattctgc agaaagcaga atcggcctat catactgcag gttcctgcaa aatgggaatt
     2821 gattcgctgt cggtagttga tccggctctg aaagtgcacg gcatacagaa tctgcgtgtc
     2881 atagattcat ctgtaatgcc tactatcaca ggaagtaata tccacagccc tacggttatg
     2941 attgccgaga agggagcgga gttgatactg aaagggaatt aaagcgggat tctatgagag
     3001 aatatccata aattccagaa gatagacggg gatataaaat atcctgtttt ctttattcca
     3061 tgccaaagta tccttggtta tgaaaataaa gtacgggata tctaaatatc tttctgaaaa
     3121 gtttttcatt gcaagagtaa tttttccctg aaccctgttt gtaaatttga tttctatcgg
     3181 aatgaacgat gaattctttc tgccgataaa gtcaatctcc gacttggaga cagttctgtg
     3241 aaaaaaaaga ttctccatct cgaatatttt tcggaaaatt cgaaaacagt atgtttcaac
     3301 gtcctgtccg gaaagaatat cataagaatt ggattccggc gttttcatta catacttcac
     3361 gattccaaaa tcttctgtat aaaccttcgg catttttgaa atttcttttc gaagatttga
     3421 aaaatacgga ggaagaaagg agaacagaaa tgtaccttcc agagcgtcca gatacc
//
LOCUS       DAOQGJ010000168         3471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15541_length_3471_cov_4.095433, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000168 DAOQGJ010000000
VERSION     DAOQGJ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15541_length_3471_cov_4.095433"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PK683_03785"
     CDS             complement(<1..245)
                     /locus_tag="PK683_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07597.1"
                     /translation="MLKKIIPTLLFLLSCSVYSIDNVLVDELEYLGASEESWMAVGIR
                     NSIINDLSGIGGKYDLNIITVKEQTEAMQSLNERRKTG"
     gene            complement(258..1580)
                     /locus_tag="PK683_03790"
     CDS             complement(258..1580)
                     /locus_tag="PK683_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH07598.1"
                     /translation="MVEDFDYQGPSDQKWISSGIKNTLASNLLRLSADLQIDVKTNQD
                     LIEAMKMAAEKRKSGININLNEEVRALIGADLVCNGKITITSNQISAKINILRPKEKK
                     NIASINISNSRDKLFELQDELLLKLIYSYKKTASIDNKLENINKLNSKNLKAIEYFTK
                     GMEFTEHKPKEAIAFFSKTLEQDENYVDALIEIGHNFIFLKDYKTALGYLNKAEKILI
                     DTNNQRTPEFARLLLNLGNINGNLYKDGSYYEKSLALYNSLGLGKTKAYLKMLINLSQ
                     AFVDGSYLNRASDLIEELNLTNTIIHADLYEAYASGYIVDKKYDLAIEYYQKALSVYE
                     KNGYKNSYKYSWFEFNIGAAYFQEKKDYHNGLNYLQKSITTLKKIDMYHSESVAETLY
                     WIGFCYRKSGNNLKAKESFLEAKRILESLDLRQSSTYSDVKNKLLDLE"
     gene            complement(1748..2623)
                     /locus_tag="PK683_03795"
     CDS             complement(1748..2623)
                     /locus_tag="PK683_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07599.1"
                     /translation="MKIKLKELILSFFCGLLIGCASTQAGTDGSSGYSSTNRNDSMSN
                     DVPVLDMNNIINLPPDLPLHSVDLIQFSGGTEKNFNPVGTWINEFQAMYTAFGEVSQF
                     SEIGFETERLEIQKGNGNTFNVKFVQSKRKRNQRATFFTDEKIVFNGKLTVKGNEAIL
                     TVKAFAADIGYKEAKSLQELEQAKFGENSQIDKSMVMNMIYLFQYDPSANVLRKMKEL
                     STPPKGSKPIMLGGKSFLGSAYTVYEYNGDRVSYKKDEVRTVPSTNMNTAAFFKGAVK
                     KGIFKNISAKSANPQ"
     gene            complement(2648..>3471)
                     /locus_tag="PK683_03800"
     CDS             complement(2648..>3471)
                     /locus_tag="PK683_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07600.1"
                     /translation="GPPSSSSAGGSSPPPAPVVIIGADGKKSEKEEQAAEDVTTRFQT
                     SCLSRLWDELTASSSSSSSSSSSSSTFPNPSQQQLQDLIEIEKISIQEKARYYHQSYI
                     DNVGTVSAHFFHENFHINDLALFSGTSFCILVSILLFSYRTEMRNLKIFFVLSRKLDS
                     LNDGYQVLAMHQVLTITPSLEPDKSEAVERIGKMIPKFLFLIPLLLEILIMFDIFLTY
                     KSVIEYNDTATMINVSFGLLFAGMVAVLTVFCFVISIQIDQLWQDTAEQIRNQQK"
BASE COUNT         1007 a          678 c          587 g         1199 t
ORIGIN      
        1 ccagtctttc ttctttcatt caaagactgc atagcctcag tctgttcttt cacggtaatg
       61 atatttaaat cgtattttcc tcctatacct gataagtcat tgataatgga atttcggata
      121 ccgaccgcca tccaagattc ttcacttgct cccaaatact caagttcgtc caccagaacg
      181 ttatcaatgg aataaacaga gcagcttaat aaaaacagaa gggttggaat gatttttttt
      241 aacattttat ctccttttta ttctaaatcc agtagtttgt ttttgacatc agagtaggta
      301 ctgctctgcc tcaaatccaa agattccaaa attctttttg cttctagaaa actttccttt
      361 gcttttagat tgtttcctga tttccggtag caaaacccta tccaatataa agtctctgca
      421 acggattcgc tatgatacat atctattttt tttaatgttg ttattgactt ttgaagataa
      481 ttcagtccat tatgatagtc tttcttttct tgaaaatatg cagcaccaat attgaattca
      541 aaccaagaat acttataaga atttttatag ccattctttt catatacact cagagctttc
      601 tgataatact ctatagccaa atcatatttt ttatcaacaa tataaccgct tgcatacgct
      661 tcgtataaat ctgcatgaat gattgtattt gttaaattca attcttcaat caaatcagac
      721 gccctgttaa gataagaacc gtcgacaaaa gcctgtgata aattgataag catttttagg
      781 tatgctttgg tcttccccag accaagagaa ttatacaggg ctagggattt ttcgtagtaa
      841 cttccatctt tatataaatt gccattaata tttccaagat tcaataaaag tctggcaaac
      901 tccggtgtac gctgattgtt cgtatcgatc agaatttttt ctgctttgtt taaatagcca
      961 agtgcggttt tataatcctt caaaaaaata aaattatgac caatttcaat caaagcatcc
     1021 acatagtttt catcctgctc aagtgtttta gaaaaaaaag caattgcttc cttgggtttg
     1081 tgttctgtaa attccatacc cttggtaaag tattcgatgg cctttaaatt cttagaattt
     1141 aatttattaa tattctccaa tttattatct atggaagcag tctttttgta actgtaaatt
     1201 agttttaaaa gcagttcatc ctgtaattca aaaagcttat cgcgagaatt actgatattt
     1261 atgcttgcaa tatttttttt ctctttaggc cgcaggatat ttatcttagc cgaaatctga
     1321 ttacttgtta ttgttatctt accattacag actaaatcag cacctatcaa agcacggact
     1381 tcttcattca gatttatatt tatcccggat tttctttttt ccgcagccat tttcatcgct
     1441 tcaatcaaat cctggtttgt ttttacatct atctgcaggt cggcggataa tcgaagaaga
     1501 ttgctggcta aggtgttttt tattcctgag gaaatccatt tctgatctga aggaccttgg
     1561 taatcaaaat cttctaccat cagtatttcg ataatcgagg agacagtttc tgattttaat
     1621 gaattctgat cattagtaac tttttgttct gaaatacagg aaaagaaaga tacaaaacag
     1681 atcactattc taatcgaaaa aaaatggatt ctagtctttg tcaaaatgaa tttcctcttc
     1741 tatcgtccta ttgcggattt gctgatttcg ctgaaatatt tttgaaaatt cctttcttga
     1801 cagccccttt aaaaaaggcg gctgtattca tattagtgct aggaactgtt ctaacctcat
     1861 cctttttata agacactctg tcaccattgt attcataaac ggtataggca ctgcccagaa
     1921 aactcttacc tccgagcata atcggctttg aacctttcgg aggagtgctt aattctttca
     1981 ttttccttaa tacatttgcg gaaggatcat actggaataa gtaaatcatg ttcattacca
     2041 tactcttatc gatttggcta ttttctccaa atttagcctg ctctaactcc tgaagtgact
     2101 ttgcctcctt ataaccaatg tctgcagcaa aggctttcac cgttaaaatt gcttcgtttc
     2161 cctttactgt gagttttccg ttgaaaacta ttttttcgtc cgtgaagaag gttgctcttt
     2221 ggttccgctt tcttttcgac tgcacaaatt tcacgttaaa ggtattgcca tttccttttt
     2281 gaatttccag tctttcagtt tcgaatccga tttcagaaaa ttgactcact tcaccaaaag
     2341 cagtatacat tgcctgaaat tcatttatcc aagtcccgac tggattaaaa tttttttctg
     2401 tacctccaga aaattgaatt aaatccacag aatgcaatgg taaatcaggc ggaagattga
     2461 tgatgttatt catatcgagt acaggcacat cattgctcat tgaatcattt ctgtttgttg
     2521 atgaatatcc agaagatcca tctgtccctg cctgtgtgct ggcacatccg attagcagcc
     2581 cgcaaaaaaa agaaagaatc aattccttca gtttaatttt catagttgtt tccttaagtt
     2641 ttatttgtta tttttgctga ttccgtattt gttctgccgt gtcttgccac agctggtcga
     2701 tctgaattga tatgacaaaa caaaaaacgg tcaggactgc taccattcct gcgaacagta
     2761 atccaaaaga aacgtttatc atggtcgcag tgtcattgta ttcaattaca gacttgtatg
     2821 ttaaaaaaat atcaaacata attaagattt ccagtaataa tggaattaag aaaaggaact
     2881 tagggatcat tttcccgatc cgctctactg cctctgattt gtccggctcc aaagaaggag
     2941 tgattgtcag aacttgatgc attgctaaaa cctgatatcc gtcgttcaac gaatctaact
     3001 tcctgcttag aacaaaaaat atcttcaggt ttctcatttc tgttctatat gaaaatagca
     3061 atatactgac aagtatacaa aaggatgtgc ctgagaacaa agctagatca ttaatatgaa
     3121 aattttcgtg aaaaaaatga gctgagacag tccccacatt gtcaatataa ctttgatgat
     3181 aatatctcgc tttttcctga atactgattt tttcaatctc gatgagatcc tgaagttgtt
     3241 gttgtgaggg gtttgggaaa gtggaggagg aggaggagga ggaggaggag gaggaggagg
     3301 aggctgtgag ctcatcccac agccgcgaga gacacgatgt ttggaagcga gtcgtcacgt
     3361 cctccgccgc ttgctcctcc ttctcgctct tcttaccatc agccccaatg attacgacgg
     3421 gagctggtgg tggtgacgac cctcctgcag atgatgatga tggtggtcct g
//
LOCUS       DAOQGJ010000169         3467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15569_length_3467_cov_3.755569, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000169 DAOQGJ010000000
VERSION     DAOQGJ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15569_length_3467_cov_3.755569"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PK683_03805"
     CDS             complement(<1..497)
                     /locus_tag="PK683_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07601.1"
                     /translation="MKNLQKAYLLIIFIFSGCTITVEEPVGGTESIVLDPRILGIWGT
                     EEQRGYQMILKDKEERNRYLLLEFDFKFGAHSQIFQLYVTKLNERTFLNYKPLHKSST
                     ESDNSYQFVSYSVQDSQLKVVIPEQSILKKAVEEKKLMGTVRESLFGNTVQLTESVSN
                     LKEFFV"
     gene            complement(540..2555)
                     /locus_tag="PK683_03810"
     CDS             complement(540..2555)
                     /locus_tag="PK683_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07602.1"
                     /translation="MSQERKNPDFPILVCVDNSLLRDYLALEFEEERFNTEFISREEL
                     VGRIPHLKKNLLVLQTDGDEFQLLETCRRIKMIHGYEARIIFLSTDYSIEEDANNVTD
                     KFMQFPVVFKDIDDAIQKFLTDTHKLLIIDDSKLVHNHLIPPLSSEGYKIYEAFDGQE
                     GLEMALEHRPDLIICDIEMPRMNGFEVCASIRSRPEISDTYIIMSSTLGSASDIQKGF
                     NAGVDEYITKPVVIGEMIERINRHFKQSLSGRENIIFLERDEQIISSFSKSLRKQGFS
                     VRAASSINETIDLVKKYSFDLIISEMELGEETAMNLCLSLKNVEMENKPSILVLTDRE
                     SNSDYKMIMNLQVSGIISKPFSTDTLLATVERVLADRRTNAERKQMLKYLSKSSAKIA
                     SEKAILSRDVKGKSEKRYTSILFADIVNFTARCEKYEPASVVEQINMIFDMIAKIIHS
                     EKGDVDKFMGDACLAYWLDSDRKAGALHLIDSILEVQKNLDEVNNASDILKSDPIRIR
                     FGANSGESILCDIGSFEARIDLTIIGDHVNLAARLEAAGKQYGIETLLSENTFLLIED
                     KVECREIDRIRVYGKSIPVPVFELFGKKGKMKAEQRELIGLFNEAKRLYIEGNFLKAK
                     NGFILSDRLEKMHPDRKTNPSRVFAQRCMYLLKNPPKNWDGTWSLGK"
     gene            complement(2552..>3467)
                     /locus_tag="PK683_03815"
     CDS             complement(2552..>3467)
                     /locus_tag="PK683_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH07603.1"
                     /translation="QGSIDLETVPGKGTVFYLKIPLTLAVVRVLLFDVGNQMLAFPMA
                     NVEEILTVSKDEIETLGNRYFYHLRSEVIPLIHMADVLSIPHSAVFKEEIPVIILNDG
                     KRKLGMVGDTILGRQEIVIKNLGTVLKKVPFIMGCAILSDRRLVMILNPGEIIEAALN
                     ESTEKKATKTISDRKEENREIHSILVVDDSPLQRKNIRSILSRAGWQVDEAENGFEAM
                     KLVRFKKYSLFCIDLVMPLMDGFELVSRLKSLPLYKSIPVIVITAKHSLEDRERGLKV
                     GANEFLEKPVNPEELTELVRKYVTAEKS"
BASE COUNT          903 a          826 c          671 g         1067 t
ORIGIN      
        1 acgaaaaact ctttcagatt agaaacagat tcagtcagtt gtactgtgtt tccgaataaa
       61 gattctctga ccgttcccat cagttttttt tcttccactg ctttttttaa tattgactgt
      121 tccggtatga caactttcaa ctgactgtct tggacggaat aggagacgaa ctgataagaa
      181 ttgtccgatt cagtgctgct tttatgaagc ggtttataat tcagaaaagt ccgttcattc
      241 aacttcgtga catacagctg aaatatttgg gaatgggctc caaatttaaa atcaaattcc
      301 aggagaaggt atctgtttct ttcctctttg tccttcagga tcatctggta acctctctgt
      361 tcttcggttc cccagatgcc gagtattctg ggatccagaa caatactttc tgttcccccg
      421 acaggttctt caactgtaat tgtgcatccg gaaaaaataa aaataatcag cagataggct
      481 ttttgaagat tcttcatatg caaacagtca gtgaaaacgg cagactgtaa agaagaaatt
      541 tatttaccaa gcgaccaggt cccgtcccag tttttaggag gattcttcag aagatacata
      601 cacctctgtg caaatacacg ggacggattt gtcttcctgt ccggatgcat tttttccagt
      661 ctgtcggaaa gaataaatcc gttctttgcc ttgagaaaat ttccttcaat atacagtctc
      721 tttgcctcat tgaacagtcc gataagttct ctctgttcgg ctttcatctt tccttttttg
      781 ccgaaaagtt caaagactgg aacaggaatg gactttccgt atacacggat tctgtctatc
      841 tccctgcatt ctaccttgtc ctctatcagg agaaatgtat tttcactcag aagagtctct
      901 atcccatact gttttcctgc agcctccagc ctcgcagcaa gatttacatg atccccgatg
      961 atagtcagat caattctcgc ttcaaaactt ccgatatcgc agagaatgct ttctcctgaa
     1021 tttgctccga accggatacg gatgggatca ctcttcagaa tatcggaggc attgttcact
     1081 tcatccagat tcttctggac ttcaagtata gaatcaatca gatgaagagc gcctgccttt
     1141 ctgtccgaat caagccagta ggccagacag gcatctccca taaacttgtc cacatccccc
     1201 ttttccgagt gaataatctt ggcaatcatg tcaaagatca tattgatctg ttccacaacc
     1261 gaagcaggtt catacttttc acatctggca gtgaaattta ctatgtccgc aaacagtatg
     1321 gaagtgtatc ttttttcgga cttccctttg acatctctcg aaaggatggc cttctctgat
     1381 gcaatctttg cagaagattt ggaaagatac ttcagcatct gctttctttc tgcatttgtt
     1441 cttctgtccg caagaactct ctccactgta gccagaagag tgtctgtgga gaacggcttg
     1501 ctgatgattc cggacacctg cagattcatg atcatcttat agtctgaatt gctttccctg
     1561 tctgtcagca caagaatgga cggcttgttt tccatctcca cattcttcag agaaagacag
     1621 agattcattg cagtctcttc accgagttcc atttcggaaa tgatcagatc aaaggaatat
     1681 ttttttacaa gatcaatggt ctcattaatg gaagatgctg ctctgacaga gaatccctgc
     1741 tttctgagcg acttggaaaa actggaaatg atctgttcat ctctttccag gaatatgata
     1801 ttttctcttc cagagagtga ctgcttgaaa tgcctgttga tccgttcaat catttcaccg
     1861 atcactacag gcttggtgat atactcgtcc actcctgcat tgaatccttt ctgaatgtcc
     1921 gatgccgaac cgagagtgct tgacataatg atataagtgt cgctgatttc cggtctgctt
     1981 ctgatcgacg cacagacttc aaatccgttc attctgggca tttcaatatc gcaaatgatg
     2041 agatccggcc tgtgctcgag agccatttcc agtccttcct gtccgtcaaa tgcctcgtag
     2101 atcttgtatc cctcggagga taaaggaggg atcagatgat tgtgcacaag cttggaatcg
     2161 tctataatca gaagcttatg agtgtcagtc agaaatttct gaattgcatc gtcaatgtcc
     2221 ttgaacacaa ccggaaactg cataaacttg tcggtgacat tgtttgcatc ctcttcaatg
     2281 gaatagtccg tggaaagaaa aatgatcctt gcttcatagc cgtgaatcat cttgattctt
     2341 ctgcatgttt ccagaagctg aaactcatcc ccgtcagtct gaagcacaag gaggttcttc
     2401 ttgagatgag gaatccttcc gaccagctct tccctgctga tgaactcagt attgaatctc
     2461 tcttcttcaa actccagtgc cagataatct ctcaaaagag aattatcgac acagactagg
     2521 attggaaaat ccgggttctt tctttcctgg ctcatgattt ttccgccgta acatattttc
     2581 tgaccagctc agtcagttcc tccggattca ccggcttttc cagaaattca ttggcaccca
     2641 ctttcagtcc cctttccctg tcttcaagag aatgcttggc agtgattaca atcacaggaa
     2701 tggatttgta gagaggcaga cttttcagtc tggataccag ttcaaacccg tccatcagag
     2761 gcattacaag atctatacag aataatgaat atttcttgaa ccgcacaagt ttcatcgcct
     2821 cgaatccgtt ttctgcctca tccacctgcc agcctgcgcg gcttaaaatc gaacggatat
     2881 tctttctctg caggggagaa tcatccacca caagtatcga atgaatttcc ctgttttctt
     2941 ccttcctgtc ggagattgtc tttgtggcct tcttttcagt gctttcattg agagcggcct
     3001 ctatgatttc tcccgggttc aggatcatca caagtcttct gtcgctcagg atggcacatc
     3061 ccatgataaa agggactttt ttcaatacag ttcccagatt tttaattaca atctcctgtc
     3121 ttcccagaat tgtatctccc accattccga gcttcctttt gccgtcattg agtatgatga
     3181 caggaatctc ctccttgaag actgcggaat gaggaatgga cagaacatcg gccatatgga
     3241 tcagaggaat gacttccgac cggagatgat agaaatatct gtttcccaga gtttcgattt
     3301 cgtccttgga aacagtcaga atctcttcca catttgccat cggaaatgca agcatctgat
     3361 ttcccacatc aaaaagaagc actctcacga cagccagagt gagaggaatc ttcagataga
     3421 aaacagttcc ttttcctgga actgtctcga gatcaatgct tccctga
//
LOCUS       DAOQGJ010000170         3457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15651_length_3457_cov_2.753380, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000170 DAOQGJ010000000
VERSION     DAOQGJ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15651_length_3457_cov_2.753380"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..985
                     /gene="ispE"
                     /locus_tag="PK683_03820"
     CDS             95..985
                     /gene="ispE"
                     /locus_tag="PK683_03820"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNH07604.1"
                     /translation="MFSPAKINFYLNILGKREDGYHELDSIFLKLSFGDDLEFFENDS
                     GEISLETVSLLNSPQKEDFELVSEKGDFTKNILYKTFQKAKTLKEIEKGVHIRLTKRI
                     PTGGGLGGGSSNAATLLKYLFPERMKNPDKEFLQFAGSIGADVPFFLYDKNCIVRGIG
                     EICEPLDLHIPNQGLLAVPKFSIPTKESFISLKKDLLKTLPSKSWKILNEDDITQLNQ
                     GNLSFFSEQFLNEFENYAFQLHPILQELKQCFYDSGAVFALMTGTGACVYSFWDKQKP
                     DLSSLKGRFPEIQFISFYVL"
     gene            988..1063
                     /locus_tag="PK683_03825"
     tRNA            988..1063
                     /locus_tag="PK683_03825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1020..1022,aa:Gln,seq:ttg)
     gene            1085..1837
                     /locus_tag="PK683_03830"
     CDS             1085..1837
                     /locus_tag="PK683_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNH07605.1"
                     /translation="MQSNSQICAVVLAAGKGTRMKSELPKVAAYLSGKPLIRHVVDNL
                     RAAGVHDIVTVVGYKKETVISLLSDYAGMRFSEQKEQLGTGHALLCTEDYLNNFKGQI
                     LVCCGDMPLISVKTFKNLMQTHISQGNVMTVLSATMENPTGYGRLIRNAKSELQYIVE
                     EKDANAEEKSVKEVNTGTYIFNSSAVYKDLKLVKSNNAQGEYYLPDLVKIYKDANGKV
                     GALKLSNAVEASGVNSPEDLANLEQILRSGKV"
     gene            1839..2774
                     /locus_tag="PK683_03835"
     CDS             1839..2774
                     /locus_tag="PK683_03835"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNH07606.1"
                     /translation="MKDLAIFSGNSNKSLAEDICKNLHIELGKASCKRFSDGEIAVKI
                     EENVRGRDVFVVQSISYPANDNLMELLLMIDALRRASARRITAVIPYYGYGRQDRKVE
                     PRVPISARMVADLIQVTGPNRVLTMDLHADQIQGFFHIPVDHLFFSPVMYDYLGSKNL
                     QDLVVVSPDSGGAERARFLGKKLNASLAIIDKRRPQANESVVMHIIGDIQNKNCLILD
                     DMMDTAGTTCKAASALLANGAKSVMCCATHGVLSGKAMDNLNSINFKEIVLSNSINIP
                     SEKKINNLTVLSVAPLFAKAIQRIHNEESISSLFL"
     gene            2798..>3457
                     /locus_tag="PK683_03840"
     CDS             2798..>3457
                     /locus_tag="PK683_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNH07607.1"
                     /translation="MSNLVLKVAQRNETGKNENNRLRAKGLIPVNIIANGKAVSGSVS
                     ESEMNKILSSGIRPATLIKLEMENGESSEVFVKDIQRFPATNQVRHIDFYKVTPGKKI
                     IAKIGIITTGTAKGSKAGGQFVHVIHEIKVKSAPEDLKDLLVLDVTNLEIGQSIKISD
                     LPTPKSWEILMNGNPIVTSVNKTKAILAAERSEKATEKDAKGGKGKAAAPAKPAAGAD
                     KA"
BASE COUNT         1075 a          648 c          783 g          951 t
ORIGIN      
        1 acagtgtatt ccctgcagct cttttgaatt tttactgact tgtgcgtgat tgttcttgtc
       61 ctttaggaca tctgcagagc ttctgaaata cagtatgttt tcccctgcaa aaatcaattt
      121 ttacctgaat attcttggaa aaagagaaga cgggtatcac gaactggatt ccatttttct
      181 gaaactgagt ttcggagatg atctggaatt ttttgaaaat gattccggtg agatcagtct
      241 ggaaacagtt tctctgttga actctccgca gaaggaggat tttgaactgg tttctgaaaa
      301 aggagacttc acgaaaaaca ttctctataa aactttccag aaggcaaaga cacttaagga
      361 aatagaaaaa ggtgttcata tccgtctgac taaaagaatt cctacaggag gagggctcgg
      421 gggaggcagt tccaatgcgg cgactctgct caagtattta tttcctgaaa gaatgaagaa
      481 tccggacaag gagttcctgc agtttgcagg ttcaatcggt gcggatgttc cattcttttt
      541 atatgataaa aactgcatcg tacgaggaat cggggaaatc tgtgagcctc tggatcttca
      601 tattcctaat cagggacttc tggcagttcc aaaattttca attcctacaa aagaatcgtt
      661 cataagcttg aaaaaagatt tactaaaaac cctgccttca aaatcatgga aaatactgaa
      721 tgaggatgat attactcagt taaatcaggg taatttgtct tttttcagcg aacagttttt
      781 gaatgaattt gaaaactatg cttttcaact gcatccaatt ttgcaggaac tgaagcagtg
      841 tttttatgat tcaggagcag ttttcgcctt gatgacaggg actggagctt gtgtgtattc
      901 cttctgggat aagcagaagc ctgatctgag ttcgttaaag ggaagatttc ctgaaattca
      961 gtttatttca ttctatgtac tgtaaattgg gctgtcgcca agtggtaagg cagcggtttt
     1021 tggtaccgcc attccctagg ttcgaatcct agcagcccag ccattactca aacggatagg
     1081 tttcatgcaa agcaattctc aaatctgtgc tgtagttctt gctgccggca aaggcactag
     1141 aatgaagtca gaacttccca aagtagcagc ctatctttcc ggaaagcctc ttatccgtca
     1201 tgtggtggat aatttgagag cagccggtgt tcatgatatt gtcacagtag tcggatataa
     1261 aaaagagaca gtgatttctc ttttaagtga ctatgccgga atgcgttttt cggagcagaa
     1321 agagcagttg ggaactggac atgctctttt atgcacagaa gattacttga ataattttaa
     1381 aggtcagatt ctggtctgct gcggggatat gcctctcatc tctgtaaaga catttaagaa
     1441 tctgatgcag acacatattt cccagggaaa tgtaatgacc gttctgtctg ccacaatgga
     1501 aaatccgaca ggatacggaa gactgattcg aaatgcaaag tccgagctgc agtatatcgt
     1561 ggaagagaag gacgcaaatg cagaggaaaa gtctgtcaaa gaagtgaata caggaactta
     1621 catttttaat tcttctgcag tgtacaagga tttgaagctt gtaaaaagca ataatgcaca
     1681 gggagaatac tatctccctg atttagtaaa aatatataag gatgcaaacg gcaaagtagg
     1741 ggctttgaaa ctttcaaatg cggtcgaagc atcgggagtc aattctccgg aagacttggc
     1801 gaatttggag cagatcctca ggtcaggtaa ggtataatat gaaagatcta gcaatttttt
     1861 cagggaactc caataaatca ctggctgagg atatatgcaa aaatctgcat atagaactgg
     1921 gcaaggcctc ctgcaaaaga ttttctgacg gggaaattgc agtcaagatt gaagagaatg
     1981 ttcgcggacg ggatgtattt gtagttcagt ccatcagtta tcctgcaaat gacaatctga
     2041 tggaactcct tctgatgata gatgctctca gacgcgcttc cgcaagaaga atcactgctg
     2101 tcattcctta ttacggatac ggaagacagg acagaaaggt ggaaccgaga gttcctatca
     2161 gtgccagaat ggtggcggat ctgattcagg tgacaggacc taacagagtg ctcacaatgg
     2221 atctgcacgc tgatcagatt cagggctttt ttcatattcc tgtagaccat ctgttttttt
     2281 ctcctgtcat gtacgattat ctgggaagca aaaaccttca ggatctggtg gttgtttcac
     2341 ctgattcagg cggtgctgaa agagcaaggt ttctcggaaa aaaactcaat gcaagtcttg
     2401 caattattga caaacgccgt cctcaggcaa acgaatctgt tgtaatgcat atcattggag
     2461 acattcagaa caagaactgt ctgattctgg atgatatgat ggacactgca ggcactacct
     2521 gcaaggctgc atctgcacta cttgcaaatg gagcaaagtc tgtgatgtgc tgtgctactc
     2581 acggggtcct ctccgggaag gcaatggaca acttaaacag cattaatttc aaggaaattg
     2641 tactttccaa ttccatcaat atcccttcag agaagaagat taacaatctt actgttctct
     2701 ccgtagcccc gctttttgcg aaggcaattc aaaggattca taatgaagaa tccatcagtt
     2761 cactgttttt ataattaaat accaaaggaa gccgagtatg agcaatttag tgctgaaagt
     2821 agcccaaaga aatgaaacag gaaaaaacga aaacaatcgt ctgagagcca aaggcctgat
     2881 tcctgtaaat attatcgcaa acggaaaagc agtatccggt tctgtttctg aatcagaaat
     2941 gaataaaatc ctgagttcag gaatccgtcc tgcaactcta atcaaattgg aaatggagaa
     3001 tggagaatct tctgaagtat ttgtaaagga tattcagaga tttcctgcaa ccaatcaggt
     3061 aaggcatatt gatttttaca aagtgactcc aggaaagaaa atcattgcaa agatcgggat
     3121 cattacaacc ggaactgcaa aaggttcaaa agccggcggt cagtttgtgc atgtgattca
     3181 tgagattaaa gtgaaatcag ctcctgaaga tctgaaagat cttttagttc tggatgtaac
     3241 aaatctggaa attgggcaga gcataaaaat cagtgatctt cctactccta agagctggga
     3301 aattctgatg aacggaaatc caattgttac ttctgtcaat aagacaaaag ctattctcgc
     3361 tgcagaaaga agtgagaaag ctactgaaaa ggatgctaaa ggcggaaaag gaaaagcagc
     3421 cgctcctgca aaaccggcgg ccggtgctga caaagct
//
LOCUS       DAOQGJ010000171         3456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15654_length_3456_cov_2.903264, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000171 DAOQGJ010000000
VERSION     DAOQGJ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15654_length_3456_cov_2.903264"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1705
                     /locus_tag="PK683_03845"
     CDS             260..1705
                     /locus_tag="PK683_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH07608.1"
                     /translation="MKKKILISVSTAILLLLFIISFPVSSGLDINEKKYSPASTLQSG
                     LPDDEIVTALSEYLKIQTVRGNEKQSALYLKKFLDKEGIESRIIEYPGKPDRANLVAE
                     LKGSENKDGIILMNHMDVVEANPSEWSFPPHSGKVENGRILGRGAIDMKGLGIMQLYA
                     FLYIKRNKIPLKRNIMYLAVADEESRSLHGATFLIEKHRDIFKGYSYMLNEGGVGTLG
                     VAFPGKKVFNIQMAEKGILWLKFFASGVSGHGSTPPPEYAALKMMRFLTKLQEMNDFP
                     VLTEDVSRFFYMMGTAAGFPDSFFLKRTKNPIVQQILKPIIKGNRHLTAMTSNTISIT
                     SINTDASSGINVIVPTVTASMDMRILPGFSPDDLLKKIETLAAQYDIKIEVIHKEAAS
                     ISDSTGELFQALAAVSKKNVPDITITPFLSPGTTDSGYFREIGIKCYGLIPALMTSED
                     LDGIHGKNESISIENLKLGTKILYETLVSLN"
     gene            1702..>3456
                     /locus_tag="PK683_03850"
     CDS             1702..>3456
                     /locus_tag="PK683_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNH07609.1"
                     /translation="MKKFQNIEEFLDFQVSLISDELAFTESENRKLRELKTVNERKKA
                     GILWNPVSLDEERYLSAGKISAKFSKSPDEISPKFKNGSPCILYSKINDFSLKGSIKK
                     ISGFSVEVYFTEDSLPDWAYEEDLVLEAVTLELSLQEMKKNLESLKLNKSQKSQELIR
                     MFAGLQEPEFVSSKTSIEIPHLNKSQNLALNSILNSEILSVFEGPPGTGKSTVLAEAA
                     KILSEQGLRILALAPSNAAADSLLSRIASEGIDVLRIGNPARFSEEAEKFSLDRKMEE
                     SDENSVIKDCRKDANELSAKANKFVRSFGAAERQKRSDLRKEARSLLKRAEQLEKSLS
                     DYILDKSNVIVTTFGACPGRLLQGRKFDAVFADEANQAVIPELLLCLDKIGGKLIITG
                     DRKQLPPVVFSESAKKAGLEKNIMELLDESDFSGQAEKNDKNRKKKSEAVSEESASGE
                     RKISVLLNEQYRMPESLFYYSNTVFYDSKVITIYPSAPEEDNFLFIDTAGADFFEEKD
                     EVNESYMNSEEASLLLQYFENFVQTEGISAGVLAPYSSQVYLLREKFKLLTDKFPLLS
                     VNTIDSFQGSEKEAILISM"
BASE COUNT         1098 a          692 c          752 g          914 t
ORIGIN      
        1 gttgacagca tccatttact tgctggtctc agcgatgact cgaatcattt ccagaaaaac
       61 cagtgaagaa atggaagaaa gactgaattc gatgcagaag aaaattgata aacttacagc
      121 cgctgctgag gcaaataaat gattggaacc atcgcttcag ttattgcagt catcgatttc
      181 attttcctca aaaagaaata atcccgttcc gaattttaag acttgatttt caaaagactt
      241 ccttcagatt tgaatgaata tgaagaaaaa aattctgatt tctgtttcaa ctgcaatcct
      301 cctccttctg ttcatcattt catttccagt cagctccggt ctggatatca atgaaaagaa
      361 gtattctcct gcttcgactc tgcagtccgg tcttccggat gatgagattg tcactgcatt
      421 gtctgaatat ctgaaaatcc agacagtcag gggaaatgaa aaacagtctg cactgtatct
      481 caagaaattt ctcgacaagg aagggattga aagcaggatc attgaatatc ctggaaaacc
      541 tgacagggct aatctggttg ctgagctgaa aggttcagag aacaaggacg gcatcatact
      601 gatgaatcat atggatgtgg tggaggcaaa tccctctgag tggtcatttc cgcctcattc
      661 aggaaaagtg gaaaacggaa gaattctcgg ccggggtgca attgatatga aagggctcgg
      721 aatcatgcag ctgtatgcct ttctatatat caaaagaaat aaaataccgt taaaaagaaa
      781 tatcatgtat ctcgccgttg ccgatgagga atcaagaagt ctgcacggtg caaccttcct
      841 gatcgaaaag cacagagata ttttcaaagg atactcctat atgctgaatg agggaggagt
      901 cggaaccctg ggagtcgcct ttccgggaaa aaaagtattc aatattcaga tggcagaaaa
      961 gggaattctc tggctgaagt ttttcgcgtc gggcgtatcg gggcacggat caacgcctcc
     1021 tccggagtat gccgcgctca agatgatgcg ttttctgacc aagcttcagg aaatgaatga
     1081 tttcccagtc ctcacggaag atgtcagcag attcttttat atgatgggaa ctgcagcagg
     1141 attccccgat tcctttttcc tgaagagaac caagaatccg attgtgcagc agatactgaa
     1201 accgatcatt aagggcaaca gacatcttac agcaatgact tccaatacga tcagcatcac
     1261 ttccatcaac acggatgcat ccagcggaat caatgtaatt gtgcctacag taactgcatc
     1321 aatggatatg agaattcttc ctggattttc acccgacgat ttactgaaaa aaatagagac
     1381 tcttgccgct cagtacgaca ttaaaattga agtcattcac aaggaggctg caagcatatc
     1441 cgacagcaca ggagaactgt ttcaggctct tgctgctgta tccaaaaaaa atgtgcctga
     1501 tatcacaatc actcctttcc tgtctcccgg aaccactgat tcaggatact tcagagagat
     1561 cggaatcaaa tgctacggtc tgattcctgc actgatgaca agcgaggatc tggacgggat
     1621 tcacggtaaa aatgaaagca tatccattga aaatctgaaa ctggggacaa agattcttta
     1681 tgaaacgctg gtctctttga attgaaaaaa tttcagaata tagaggaatt tcttgacttt
     1741 caggtaagtc tgatttcaga cgagctggca ttcactgagt ctgaaaacag aaaacttaga
     1801 gagcttaaga ccgtaaatga aagaaaaaag gcagggattc tctggaaccc tgtctctctg
     1861 gatgaagaaa gatatctgag tgcaggaaaa atatcagcaa aattttccaa gtctcccgat
     1921 gaaatctctc caaaattcaa aaacggaagt ccatgcattt tatattctaa aattaatgat
     1981 ttttcattaa aaggaagcat taaaaaaata tcaggattca gtgtcgaagt ctattttacg
     2041 gaggattctc ttcctgattg ggcttatgag gaagatcttg ttcttgaggc tgtaaccctc
     2101 gagctttccc ttcaggaaat gaaaaagaat ctggaatcat taaagctgaa taaatcacag
     2161 aaatcgcagg aactgatcag gatgtttgca ggacttcagg aacctgaatt cgtatcatca
     2221 aaaacttcaa ttgaaattcc ccatctgaac aagtcgcaga atcttgcatt gaattccatt
     2281 ttgaattctg agattctttc cgtatttgaa gggcctcccg gaacaggcaa atccactgtt
     2341 cttgcagagg cagcaaaaat tctttcagag cagggactgc gaattcttgc acttgcgccg
     2401 agcaatgcag cagcagattc tcttctttcc aggattgctt cggaaggcat tgatgttctt
     2461 cggatcggaa atcctgcacg tttttcagag gaggcggaaa aattcagcct ggacagaaaa
     2521 atggaagagt ctgatgaaaa ctctgtaatc aaagactgca gaaaagatgc aaatgagctt
     2581 tctgcaaaag caaacaagtt tgtccgcagc ttcggcgcag cagaaagaca gaaacggtcc
     2641 gacctgagaa aagaagcaag gtctttactt aagagggcag aacagctgga aaaatccctc
     2701 tccgattaca tattagataa atctaatgtc attgtaacca cattcggagc ctgcccagga
     2761 aggcttcttc agggaagaaa atttgatgct gtatttgcag atgaggcaaa ccaggcagtg
     2821 atcccggaac ttttactctg cctcgataag attggaggaa aacttataat cacaggtgac
     2881 agaaagcagc tccctcctgt ggttttctct gaatcagcca aaaaggcagg actcgaaaaa
     2941 aacattatgg aacttttgga tgagtctgat ttttcgggac aggcagaaaa aaatgataag
     3001 aatcggaaaa agaaatcaga ggcagtctcg gaagaatctg cctccggtga aagaaagatc
     3061 agcgttcttc tgaatgagca gtacaggatg ccggaaagtc ttttttatta ttccaataca
     3121 gtattttatg attcaaaagt aattacaata tatccttcag cacctgaaga ggacaatttc
     3181 cttttcatcg acactgccgg agcggatttt ttcgaagaga aggacgaggt caatgaaagt
     3241 tatatgaatt ctgaagaggc ttccctgctt ctgcagtatt ttgaaaactt tgtgcagact
     3301 gaaggaatat cggcaggggt tcttgcaccc tactcttctc aggtctacct tctccgggaa
     3361 aagttcaagc ttctgactga taaatttcct ttattatctg tgaatacgat agattcattt
     3421 cagggttctg agaaagaagc aatccttatc agtatg
//
LOCUS       DAOQGJ010000172         3455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15656_length_3455_cov_5.664706, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000172 DAOQGJ010000000
VERSION     DAOQGJ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15656_length_3455_cov_5.664706"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..948
                     /locus_tag="PK683_03855"
     CDS             58..948
                     /locus_tag="PK683_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07610.1"
                     /translation="MSLNLEALDQEVSKEKLKLKATISGISHSGKTGTALMLAEKLAD
                     GDWTKVLAVDVGERNGVSVYEYLGKFRTIKIPPPYTLNLITECIQYAVGKGYKVIIFD
                     SLTQFWSGVGGCLEQADKIGLKNNPWSKISPALRKTMLDALDADIHCIFTLRQKSDKI
                     EEINEVTNKKVMKKVGMKDDFKPDSDYDFDLCIEMDREHKAIAHKDRTRICPDNEFFE
                     FTSDTASQIAEWTQKGKDVIGEMIAEAESLGIKEGNIVSKEAFIFANDNKSRLPKYMK
                     LWYTEKDPTTGERKWVKFTK"
     gene            1004..2224
                     /locus_tag="PK683_03860"
     CDS             1004..2224
                     /locus_tag="PK683_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07611.1"
                     /translation="MKLFKEIADLSMDQKEEISLLNAKYDQLALEHFSISSLNSFREN
                     RMTWAVRYLLKKYSSYPKPSMIRGQALEAALWNYVSGSSTDSRVYMDRYFKTKTLLQI
                     IGESKKAGLYPVIPPEWKNKQGQIAGAISQFGDEKYWQTVLDIFENLHKIQGDSLREY
                     MLPDSLESLYKKMKREYQFIHSSFDDLIEYGEKFRSRIDSVKYQNRVTGYIAKIEIPV
                     IGFTDFELPEYGVDLKTSLTKPNEKKLSQSHICQCAFYSEIQNKPWKLAYVTPVSSDD
                     KKKSVFHKLYSRGLSADDIAKQYKNFSPDGKGTTKPTVEKFLTELLEGSWKEPKGVTE
                     IEVSEKQTAQGKILNQLTARAIQHIINSCKQENLIEDMKIFCVDKPDGMMADPIEKEM
                     IREVWGLDSEEEQD"
     gene            2269..2997
                     /locus_tag="PK683_03865"
     CDS             2269..2997
                     /locus_tag="PK683_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07612.1"
                     /translation="MDLIKYDAGTLPAVTMPEWKPEKVTSFQELRTAEQRQKELTKLR
                     TEFISSFKPVKQEIDKIKQQALDYEKAEVEKVTSVEDMNAKFISAFKTEVGTLQSELK
                     ALVASYYQIFEKCKYEISAHSRKMESVDIELDRVQIQNSKELAVEKAKKLESLEKTEL
                     KIEIQNIQPHQTAPEVKILKKKDEIKGKEVYSIANQEAFIFWAIHNNPSLLKIEVKKA
                     EVNKWLQSPENQTLPFVKKETSFS"
     gene            3078..>3455
                     /locus_tag="PK683_03870"
     CDS             3078..>3455
                     /locus_tag="PK683_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07613.1"
                     /translation="MAGFDNLKGTTSDSFFIGKFSNKIFLRSNSGTIEAKNSTSPYFP
                     LSRGSFTAKYTILTDFAAGEVLDLATGSGSISGTCTVIGQPANLSLPANATLFNESAE
                     IQIYINGVLAERGIDVVWVSQTSI"
BASE COUNT         1214 a          679 c          765 g          797 t
ORIGIN      
        1 ggaatatgtc agctttgcac tgactgtatt ggcaaatcaa atttaaggag gacaaaaatg
       61 agtttgaacc tagaagcact agaccaagaa gtcagtaaag agaagctgaa gctgaaagcg
      121 acaatcagcg ggatcagtca ctcaggaaaa acagggacag cattaatgct tgctgaaaaa
      181 cttgcagatg gtgattggac aaaagttcta gcagttgacg tcggcgaaag aaacggggta
      241 tctgtatatg aatacctcgg aaagttcagg acaatcaaaa ttccgcctcc ctacactctg
      301 aacctcatca ctgagtgcat tcagtatgca gtgggcaagg gatacaaggt cattatcttt
      361 gactccctta ctcagttttg gtcaggggtt ggcgggtgcc tagaacaggc ggacaagatt
      421 ggtctgaaaa acaatccttg gagcaagatt tcacctgctc tcaggaagac catgctcgac
      481 gctcttgacg cagacattca ctgcatattt accctcaggc agaaatctga caagatcgaa
      541 gaaatcaatg aagtcacgaa taaaaaggtg atgaaaaaag tcggaatgaa agacgatttt
      601 aaaccggact ccgattatga ctttgacctc tgcatcgaaa tggatagaga acacaaggca
      661 attgctcata aggacagaac tcgaatctgt ccagacaatg aattctttga atttacctca
      721 gacaccgctt ctcagattgc agaatggacg caaaagggga aagatgtgat aggagaaatg
      781 attgcagaag ctgaaagtct tggaatcaaa gaaggaaaca ttgtatcaaa ggaagctttc
      841 atttttgcca atgacaataa aagcagactt ccgaaataca tgaagctttg gtatacagaa
      901 aaagatccga caaccggcga aaggaagtgg gtcaaattta caaaataaaa aaatcacttc
      961 aggcaggttg actcctgccg atactttcac aaaggacaaa aatatgaaac tattcaaaga
     1021 aatcgccgat ctgtcaatgg atcagaagga agaaatcagt cttctcaatg caaagtatga
     1081 ccagcttgcc ttggaacatt tttcaatttc cagcctgaac tcattcaggg aaaaccgcat
     1141 gacttgggca gtaagatatt tgctcaaaaa atacagttcc tatccaaagc cgtcaatgat
     1201 tcgtgggcaa gctctggagg ctgctctgtg gaattatgtt tccggcagca gcacagattc
     1261 cagagtttat atggacaggt attttaaaac gaaaacactt ctccagatta tcggagaaag
     1321 caagaaagct ggactgtatc ctgtcatccc gccggaatgg aaaaacaagc aaggacagat
     1381 tgcaggggca atttctcagt tcggtgatga aaaatactgg cagactgttt tggacatctt
     1441 cgagaacctg cataaaattc agggagattc tctaagggaa tatatgcttc ctgacagtct
     1501 ggaaagtctg tataaaaaaa tgaaaaggga ataccagttt attcacagct cttttgatga
     1561 cctgatcgag tatggcgaga agttccggtc aagaattgat tcagttaaat atcaaaacag
     1621 ggtcaccgga tacatcgcga aaatcgaaat tccagtcatt gggttcactg actttgaact
     1681 ccctgaatat ggggtagatc tgaaaacatc tctgacaaag ccaaacgaaa agaaactgtc
     1741 tcagtcacat atctgccagt gtgcgtttta ctccgaaatt cagaataagc cgtggaaact
     1801 tgcttatgtg actcccgttt catcggacga taagaaaaaa tccgtgttcc acaaactcta
     1861 cagtcgggga ctgtctgctg atgatatcgc aaaacagtat aagaacttct ctccagacgg
     1921 caagggcaca actaagccaa ccgtagaaaa atttctgact gaactcttgg aaggctcatg
     1981 gaaggagcct aaaggagtga ccgaaattga agtaagcgaa aaacagacag cacaggggaa
     2041 aatccttaat cagcttactg caagagctat ccagcacatc ataaatagtt gcaaacagga
     2101 aaatctgata gaagatatga agattttctg cgttgacaag ccagacggga tgatggctga
     2161 tccaatagag aaggaaatga tcagagaggt atggggactc gattcagaag aagaacagga
     2221 ctaaagtatt cggcaattgc agccgacaaa aaattaaagg agaaaataat ggatcttatc
     2281 aaatatgatg ctggcacact gccagctgtc acaatgccgg aatggaagcc ggaaaaagtg
     2341 acctcgtttc aggaactcag aacagctgaa caacgtcaga aagaactgac taagctcagg
     2401 acagaattca tcagcagttt taagcctgta aagcaggaaa tcgacaaaat caaacagcag
     2461 gctctcgact acgagaaagc cgaagtggaa aaagtcactt cggttgagga tatgaatgca
     2521 aaatttatca gcgcattcaa gactgaagtt ggaactttgc agtcagaatt gaaagccttg
     2581 gtcgcttcat actatcagat tttcgaaaaa tgcaaatatg agatttcagc acactccaga
     2641 aagatggaat ctgtggacat agaacttgac agagtgcaga ttcagaacag caaagaactg
     2701 gcagtggaaa aagctaagaa actggaaagc ctggaaaaga ctgaattaaa aattgagatt
     2761 cagaatatcc agccgcatca gaccgctcca gaagttaaaa ttctgaagaa aaaagatgaa
     2821 atcaaaggga aggaagttta cagcattgct aatcaagagg cattcatatt ttgggctata
     2881 cataacaacc cgtcgctgct caaaattgag gtcaaaaaag ctgaggtaaa caaatggctc
     2941 caaagtccag aaaaccaaac attacctttc gtaaagaaag agacctcatt ttcctgagac
     3001 taaaaactgc cttggaagaa attctaaggc aggtcaaaca aacttgactt aaaaaaatcc
     3061 agtcataaaa ttcaattatg gctggattcg ataacctcaa aggcactacc tcagactcat
     3121 ttttcatagg caaattttca aacaaaattt ttctcagaag caattccggc actatcgagg
     3181 ccaaaaattc aaccagtcct tattttccgc tttcgagggg aagttttaca gcaaaatata
     3241 ctatcctgac tgatttcgca gcaggggaag tgctggactt ggctactggc agcggatcaa
     3301 tatcaggaac gtgcacagta atcggacagc ctgcaaacct gtccttgcct gctaatgcta
     3361 cactgttcaa cgagtctgca gagattcaga tttacatcaa tggggttcta gctgaaagag
     3421 gaatcgacgt tgtatgggta tctcagacgt caata
//
LOCUS       DAOQGJ010000173         3455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15669_length_3455_cov_2.666176, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000173 DAOQGJ010000000
VERSION     DAOQGJ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15669_length_3455_cov_2.666176"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /gene="rpsE"
                     /locus_tag="PK683_03875"
     CDS             complement(<1..200)
                     /gene="rpsE"
                     /locus_tag="PK683_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560639.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNH07614.1"
                     /translation="MAHMEEEAKEFSEKVVKIDRVAKVVKGGRRFSFNALTVVGDTKN
                     RIGIGFGKANEVPDAIRKSIESA"
     gene            complement(200..568)
                     /gene="rplR"
                     /locus_tag="PK683_03880"
     CDS             complement(200..568)
                     /gene="rplR"
                     /locus_tag="PK683_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNH07615.1"
                     /translation="MISRKGIQESSARRTERIRYKLKKNGDRPRLVLNKTNRYLIAQI
                     VDDTNGTTLAYAMSSEKDFPVQGYSRKNKKSAAELGKILAQRAKGKGVTKVMFDRSGK
                     IYHGNLAAFADSAREGGLEF"
     gene            complement(572..1111)
                     /gene="rplF"
                     /locus_tag="PK683_03885"
     CDS             complement(572..1111)
                     /gene="rplF"
                     /locus_tag="PK683_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNH07616.1"
                     /translation="MSRVGKEPVKLPPKVEVKQVGGSIEVKGPLGTLQTPIYDGIQME
                     QDNGVVKFKRNSEEQNIVALHGLVRALFNNSVKGVTQGWSKNLEIFGVGYRAQKKGTN
                     LVMSLGFSHEVVFPEPPGIKIEVADQLKIKITGIDRQLVGQTAANLRGLKQPEPYKGK
                     GIKYSDEVIRRKAGKTGKK"
     gene            complement(1131..1526)
                     /gene="rpsH"
                     /locus_tag="PK683_03890"
     CDS             complement(1131..1526)
                     /gene="rpsH"
                     /locus_tag="PK683_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNH07617.1"
                     /translation="MLTDPISDMLTRIRNAGRAKHNSCKVSGSRIKKSILDILKDEGF
                     IKEYQEVKENNISNLEVFLKYDMKKKPVISQIDRNSRPGRRIYIKAEDVKPVRSNMGI
                     SILSTSKGVMTNKKAKTLKIGGEVICTVF"
     gene            complement(1539..1724)
                     /locus_tag="PK683_03895"
     CDS             complement(1539..1724)
                     /locus_tag="PK683_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNH07618.1"
                     /translation="MAKKSMIARHAKKKKFKVQEYNRCGVCGRPRAYLRRFGMCRLCF
                     REYAGNGQIPGVRKASW"
     gene            complement(1734..2282)
                     /gene="rplE"
                     /locus_tag="PK683_03900"
     CDS             complement(1734..2282)
                     /gene="rplE"
                     /locus_tag="PK683_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNH07619.1"
                     /translation="MSTVPRLKTKYLKEIKPSLVEKYKYSSTMEAPKLEKIVINVGMG
                     DGHTNPKALESALETLGLITGQRAVKTKAKKSIAGFKLRENMNIGCMVTLRGNKMFEF
                     LDRFINVALPRVRDFKGVNPKGFDGRGNYNMGIKEQIIFPEIQFDKVHSIHGMNIAFI
                     TSSKTNDEAFDLLKGFGMPYKK"
     gene            complement(2286..2639)
                     /gene="rplX"
                     /locus_tag="PK683_03905"
     CDS             complement(2286..2639)
                     /gene="rplX"
                     /locus_tag="PK683_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNH07620.1"
                     /translation="MAKKLPYRGSEPTKFKKIKLKKDDEVIIISGKKEDKGKVGKILS
                     IDKKKDRVFVSGINKRKKFVRPTQENPQGGVVEIEQSIHISNVMLYDTKVKKGVRVGF
                     QGEGKKKERVSRGKK"
     gene            complement(2639..3031)
                     /gene="rplN"
                     /locus_tag="PK683_03910"
     CDS             complement(2639..3031)
                     /gene="rplN"
                     /locus_tag="PK683_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNH07621.1"
                     /translation="MIQQETVLQVADNSGAKRVMCIKVLGGSKKRYATIGDEIIVAVK
                     DAQPAYGLRDGIGKKVHAKAVQRAVVVRTKKEIRRADGTYIRFDDNAVALIDDKGSPK
                     GTRIFGPVARELRDKKYMKIVSLAPEVL"
     gene            complement(3033..3293)
                     /gene="rpsQ"
                     /locus_tag="PK683_03915"
     CDS             complement(3033..3293)
                     /gene="rpsQ"
                     /locus_tag="PK683_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNH07622.1"
                     /translation="MENTEKTRKRVQGKVVSAKMKKTVVIEFETRHTHSKFKKITKKT
                     TRLKVHDENSQCKEGDIIIAEETRPLSREKRHRLFKIVMEAK"
BASE COUNT          808 a          836 c          575 g         1236 t
ORIGIN      
        1 gcagactcaa tcgacttgcg aattgcatca gggacctcat ttgcttttcc aaaaccgatt
       61 ccgattctgt ttttagtatc ccctacaaca gtcagtgcat taaaggagaa acgacgtcca
      121 ccttttacca cttttgcaac gcggtcaatc ttgaccacct tctcggaaaa ttcttttgct
      181 tcttcttcca tatgtgccat tagaattcca agcctccttc tctagctgaa tctgcaaagg
      241 ctgccaaatt tccatgatag atctttccag atctgtcaaa cataaccttt gttacacctt
      301 ttccttttgc tctttgtgca aggatctttc caagctcagc tgcagacttt ttattctttc
      361 tggaataacc ctgcacagga aaatctttct ctgatgacat tgcatatgcc aatgtagttc
      421 cgtttgtatc atctacaatc tgagcaatca gatatctgtt agtcttgttc aaaaccagac
      481 gaggacgatc cccgtttttc ttgagtttat aacgtattct ctcagttctt cttgcgctgg
      541 attcctgaat tccttttctt gaaatcataa cttatttctt ccctgtctta ccggctttac
      601 gacgaatcac ttcgtcactg tatttgattc ccttaccttt gtaaggctcc ggctgcttta
      661 atcctctaag gttcgcagct gtctgaccga ctaactgtct gtcaatccct gtaatcttga
      721 tcttcagctg atctgccact tcaattttaa ttccaggagg ttccggaaat acaacttcat
      781 gagaaaatcc taaactcatt actaagttag ttcctttctt ctgagctctg taaccgactc
      841 caaaaatttc aaggtttttg ctccaaccct gagttacacc ttttacagaa ttgttaaaga
      901 gagctcttac caaaccatgc agtgctacga tgttctgttc ttcgctgttt ctcttaaatt
      961 taacaacacc attgtcctgc tccatttgaa tcccatcata gataggagtc tgaagagttc
     1021 ctagcggacc cttcacttca atagagccgc ccacttgttt tacttccact ttaggcggaa
     1081 gtttaactgg ttcttttcct actctggaca tatttcacga tttcatctgc ttagaataca
     1141 gtgcagatta cttctcctcc gattttcagc gtcttcgcct tcttgtttgt cattacacct
     1201 ttggaagtag agagaatgga aattcccata ttgcttctga ccggtttgac atcttcggct
     1261 ttaatataaa ttcttcttcc tggacgggaa tttctgtcaa tttgcgaaat gacaggcttc
     1321 tttttcatat catacttcag gaaaacttcc agattagaaa tattgttctc ttttacttcc
     1381 tgatattctt ttataaaacc ttcatctttc aggatgtcca agatggattt tttaattctg
     1441 cttccggaaa ctttgcagga attatgtttt gctcttcctg cgtttctgat tctagtcagc
     1501 atatctgaaa ttggatctgt taacatacta ttcctctctt accaagatgc ctttctgacg
     1561 ccagggattt gcccgttgcc ggcatattcg cggaaacaga gacggcacat accaaatctt
     1621 ctcagataag ctcttggacg tccacaaact ccgcatctgt tgtattcctg caccttgaat
     1681 ttcttctttt tggcatgacg ggcgatcatc gatttcttag ccattctatt ctcctacttc
     1741 ttgtaaggca ttccaaagcc tttcagcaaa tcaaaagctt catcatttgt tttagatgaa
     1801 gtgataaacg caatgttcat accatgaata ctgtgaacct tgtcgaactg aatctcagga
     1861 aagataatct gttctttaat gcccatatta taattccctc tgccgtcaaa tcccttagga
     1921 tttacacctt taaagtcccg aaccctcggt aaagccacgt ttataaaacg gtccaggaat
     1981 tcaaacattt tgttcccgcg aagagtaacc atacatccga tattcatatt ttctcttaac
     2041 ttgaacccgg cgattgactt cttcgcttta gtcttcactg ccctctgacc ggtaatcagt
     2101 ccgagagttt ccagcgcaga ttcgagagcc ttagggtttg tatgcccgtc tcccatccct
     2161 acgttgatta caattttctc caactttggt gcttccattg tagaagagta cttgtatttt
     2221 tccaccagtg aaggcttaat ttcttttaaa tactttgttt taagtctagg tacggtgctc
     2281 ataaattatt ttttcccccg ggaaacacgc tctttctttt tgccttcgcc ctggaaacct
     2341 actcttacac ctttcttaac ttttgtgtca tagagcatta cattggaaat atgaattgac
     2401 tgctcaattt ccacaactcc gccttgcggg ttttcctggg tcggtcttac aaactttttg
     2461 cgtttattga tgccgctcac aaaaactctg tcttttttct tgtcgatgga cagaatcttt
     2521 cctacttttc ccttatcttc ttttttacct gaaatgatga tgacttcatc atcttttttc
     2581 agtttgatct tcttgaactt agtaggttca gatcctctgt aaggaagttt cttcgccatt
     2641 ataacacctc cggtgcaaga gatacaatct tcatatattt cttgtctctc agctctctgg
     2701 caactggccc aaagatacga gtcccttttg gacttccttt gtcgtcaatc agtgcaaccg
     2761 cattgtcatc gaagcggata tatgttccat ctgctcttct gatttctttt ttagttctga
     2821 ccacaactgc tctctgaact gcttttgcat gaaccttctt accgattcca tcacggagtc
     2881 cgtatgcagg ctgagcatct tttactgcaa cgatgatctc atcaccgata gttgcgtaac
     2941 gttttttgga gccgccgagt accttgatgc acattactct ttttgctccg gaattatctg
     3001 ccacttgaag gactgtttcc tgctgaatca tattacttag cctccattac aattttgaaa
     3061 agacgatgac gtttttctct ggataaagga cgagtctctt ccgctatgat aatatcgcct
     3121 tctttgcact ggctgttttc atcatggact ttcagacgag tagtcttttt agtgatcttc
     3181 ttgaattttg aatgtgtatg acgagtttcg aactcgatga caacagtctt tttcattttc
     3241 gcactgacta ctttgccctg tactcttttg cgtgtttttt cggtattttc catacagtat
     3301 taccctttct ttccttttac agcaggcgcc gctttcgttg ctgtcttgga agctgcaatc
     3361 tcgcgctctc tttgaattgg ctgaagtcta gcaatcttct ttttcagatt acgaattttc
     3421 ttaaatgctt catctacttt aagcaaaagg gggca
//
LOCUS       DAOQGJ010000174         3439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15815_length_3439_cov_3.618203, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000174 DAOQGJ010000000
VERSION     DAOQGJ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15815_length_3439_cov_3.618203"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PK683_03920"
     CDS             complement(<1..1074)
                     /locus_tag="PK683_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH07623.1"
                     /translation="MEKQAVEIIVSGPGYGKRKTDIVPGIYKSDDGKLYLDAKELENL
                     SRQSRNLNLLIDSARSIMAEISLDTLLDLIIQTVKSVMNADRATLFLIDNEKKQLWSR
                     IAIGTKQEIRIPFGIGIAGYVAQSRETVNIQDAYSDSRFNPENDKRTGYRTKSILCMP
                     VYNSQTEIIGVIQVLNKLTAEHFTEEDEKLLGAYASLAGISLANAQAYDELQKERDLL
                     DVRVKERTKDLEESRKKSDELLLNILPAEIANELKGNGLVTPKKYEVVTVLFTDFKGF
                     TLAAEKMAPENLVDELDKCFRHFDGIVEKNNLEKIKTIGDAFMCAGGIPMENRTNPVE
                     SVLAALEICKYMNDLKAEKEKKGL"
     gene            complement(1557..2186)
                     /locus_tag="PK683_03925"
     CDS             complement(1557..2186)
                     /locus_tag="PK683_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07624.1"
                     /translation="MNTTKFLAILSVLGFSLSGFLFLKNQKEEKACREEISKQDLNPK
                     DEGADGGISISEILERNTVPAWRSVKFFNTAVSIPYSEKWKIRSVGISAFDEEGENEH
                     RIYRFGKPMDGGTLIEREYFLERSEKKNLETLLKELSGGCGEGVASEKIQIGSIYGVK
                     HYAGGARGCSVGIAFVTGKHTFSLYRVSDIGQDAPEISEEMKKIILSIH"
     gene            2399..>3439
                     /locus_tag="PK683_03930"
     CDS             2399..>3439
                     /locus_tag="PK683_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07625.1"
                     /translation="MEISDKENISKILNSVFTRNPVRYLSDDGKTALCDFIGYESSLI
                     KIKAPKPDLKTSQRILISTQGKIFICKCEVASKDDSFELLRPLRIVVKDEGDTQEKIQ
                     AVENKEIKNVSVTNILSQNDVTKFLSDDKIKEIIKSNSSRLSHLFSKYNIYLNERADD
                     RLRMMHHYDQPIFIPNRDDKLSVPDGFIPHYEYIRSAKPDKNDGMIAEICIPIKYRQF
                     STIGYVQVMNSSRLDLNAFNLVSLVAAAIKKEISLYSNYEESKEICKVYDITQTDISF
                     FHSNNRHYSRIFYLGDIIIFDLVISKNRRITMRGVIRQIKALDSGFKIYCQYHNLTLQ
                     QLELIEGYLEESA"
BASE COUNT          992 a          705 c          639 g         1103 t
ORIGIN      
        1 gaggcccttt ttttcttttt ctgctttcaa atcgttcata tacttgcata tttcaagcgc
       61 agcaagaaca gattccaccg gatttgttct gttttccatt ggtatccctc cggcacacat
      121 aaaggcatca ccgattgttt tgattttttc cagattgttc ttttcaacaa tcccgtcaaa
      181 atgacgaaaa catttatcca gttcatccac cagattttcc ggtgccattt tttcggcagc
      241 taaagtaaat cctttaaaat cagtgaacag gacagtgacg acttcatatt tttttggtgt
      301 gaccagcccg ttgcctttga gctcattcgc aatttctgca ggaagaatat tgagcagaag
      361 ctcatcggat ttctttctgg attcttcaag gtctttggtc ctttccttga ccctgacatc
      421 cagaagatct ctttccttct gaagttcatc ataagcctgg gcatttgcta aagaaattcc
      481 ggcgagtgat gcgtaagccc ctaggagttt ttcatcttct tccgtaaagt gctctgctgt
      541 aagtttgttc agaacctgaa tgacgccaat gatttctgtc tgtgagttgt atacaggcat
      601 gcagagtatg gactttgtcc tgtaacctgt tctcttgtca ttttccggat tgaatctcga
      661 atcagaatag gcatcctgaa tattcactgt ttccctagac tgagcaacat atccggcaat
      721 ccctataccg aagggtattc tgatctcctg tttagtgcca atggctatcc ttgaccaaag
      781 ctgttttttt tcgttgtcaa tcagaaacag tgtcgctctg tctgcgttca tcacactttt
      841 tactgtctga atgataaggt caagaagagt gtccagactg atttcagcca taattgacct
      901 ggctgaatcg atcagcagat tcagatttct ggactgccga ctcagatttt ccagttcttt
      961 ggcatccaga tacagttttc catcatctga tttgtatatg cctgggacga tgtctgtctt
     1021 tcttttcccg tacccaggtc cggatactat gatttctact gcctgttttt ccactgtata
     1081 tcaaaaaatc gggagtatgg cttttactgt caagaaggaa tgatttgaaa taaaatataa
     1141 gtcttgttta ttttcaaaga gtttttattt ctttatatag aattaaaaat cagtgtaaaa
     1201 gaatcgaaat tcattccttc tcaaaaaaag cctgctgata ccaaaagctc accctcatac
     1261 gttgaaaaat gggcataccg ccggtgaatt gtcagcaagt gacccgctta gtttttagat
     1321 gctttcagca gacttgctct catttttaaa tgaaagccta aatatcaggt tcttttcagg
     1381 agcaggaagt ttcgcagacg gtctttgctg ataaaaaaga ttataaaatt cttatcgaag
     1441 tgcctgtttt gagtcactta tgattattga gaacggcatc aatgtcgtta aacttttgcc
     1501 gcttctgaat attttgatgc cggaagttaa atgcccaaaa tacgactctg tttgaatcag
     1561 tgaatgctta aaatgatttt tttcatttct tcgctgattt ctggagcatc ctgaccgata
     1621 tcagaaactc tatacagaga aaaggtatgt tttcctgtta cgaatgcaat tcctacagag
     1681 cagcccctgg cacccccggc ataatgtttc actccataaa tacttccgat ctgaattttt
     1741 tccgatgcaa ctccttctcc gcatccgcct gagagttcct tcaatagtgt ttccaggttc
     1801 ttcttttctg atctttccag gaaatattct ctctcaatca gagtgccgcc atccatcggt
     1861 tttccaaatc ggtaaattct atgttcattt tcaccttctt catcaaatgc cgaaattccg
     1921 acagaacgga ttttccattt ttcggagtaa ggaattgaga cagcggtatt gaagaatttt
     1981 actgatctcc aggcgggaac agtatttctt tccagtatct cagatatgga aatgcctccg
     2041 tctgcgcctt catcttttgg attcaaatcc tgttttgaaa tttcttccct gcaggctttc
     2101 tcttccttct gattttttaa aaacagaaag ccggaaaggc tgaagccgag tacagacagt
     2161 attgccagaa attttgttgt attcataaac acctgcctac atttctattt gtcgaaatca
     2221 tacaatacct gcaaggcact cacagcagaa agaaaaaaat aatgccttgc tgagtgcgga
     2281 ttgtctgtca aattcggtaa actaattatt tttggacttg cgtaaaaaaa cactcgcaat
     2341 gagacagacc gaatctatcg ttgggatttg gaaaaatcat acttaatgga atttgggcat
     2401 ggaaatatca gataaggaaa acataagtaa aatactgaac tcagttttta caagaaatcc
     2461 tgtacggtat ctttcggatg acgggaaaac tgcactttgc gattttatcg gctatgaaag
     2521 ctcacttatc aaaatcaagg ctccaaaacc tgatttgaaa acatcccaaa gaatcctgat
     2581 cagcactcag gggaaaatat ttatatgcaa atgtgaagtt gcctctaaag acgattcttt
     2641 tgaactgctt cgtccattga gaatagtggt taaagatgaa ggtgatactc aggaaaaaat
     2701 acaggcagtc gaaaacaaag aaatcaagaa tgtcagtgtt acaaatattc tttctcagaa
     2761 tgatgttacc aaatttctga gcgacgacaa gattaaggaa atcattaaat ccaattccag
     2821 cagactttcc catctgtttt caaagtacaa tatttatctg aatgaaagag ctgatgacag
     2881 acttcgaatg atgcaccatt atgatcagcc tatttttatt ccgaacaggg acgataagct
     2941 gtccgttcct gacgggttta ttcctcacta tgagtatata agatctgcaa agcctgataa
     3001 aaatgacgga atgatcgctg aaatctgcat tccgatcaaa tacagacagt tcagcacaat
     3061 cggttatgtc caagtaatga attcttcaag actggatctg aatgcattta atttggtgag
     3121 ccttgttgcc gctgcaatca aaaaggaaat ttctctctat tcaaattatg aagaatccaa
     3181 ggaaatctgc aaggtttacg atattactca gactgacatc agtttttttc attcgaacaa
     3241 cagacattat tccagaattt tttatcttgg tgatatcatt atttttgatt tagtcatatc
     3301 aaagaacaga agaattacaa tgagaggggt catcaggcag atcaaggcac tggattcggg
     3361 ctttaagatt tactgccagt atcataatct cactctccag cagcttgaac tgatcgaagg
     3421 ttatctggaa gagagtgct
//
LOCUS       DAOQGJ010000175         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15826_length_3438_cov_3.287910, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000175 DAOQGJ010000000
VERSION     DAOQGJ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15826_length_3438_cov_3.287910"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PK683_03935"
     CDS             complement(<1..993)
                     /locus_tag="PK683_03935"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HNH07626.1"
                     /translation="MKYCIGTVFFLFVFFPALNLYSQENIILSSFQPFESEKNPETEK
                     EIHSILSEKIRNSGFTVQSPEGISLSERLAAAKQSKAYLLIEGFYSKKNPDSNLNLYI
                     QLYNPETGKIIDAYSVTDEVFQSEGLNLDKEELRETDRTVISKLAAKTSFVLRSNPGR
                     KENISNIEQYVNSSRLSEKLKPFMQTEEKAAEKAAAEVFDLMQGQQVTVSSTKVAKKT
                     SEAPNIVSVVSDRQIRDYGRISINDILYQLPGFAASQINDRRTVSARGMYEGWNNNHL
                     LMLVDGVQHNDLFYGSALTWEITPLNMIKSLEVIRGPGSALYGSNATNGVISLNT"
     gene            1628..3163
                     /locus_tag="PK683_03940"
     CDS             1628..3163
                     /locus_tag="PK683_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH07627.1"
                     /translation="MKIFLLLFLNFLFLSSTYAAEKITKANLLSASASPILIITSLGC
                     DMGQGYVMAHESNLDDLKGVKIGWADINDIYQNRLFESAGSVEIGDILLVKDGKILDT
                     SKDADAEDSIIAGNIAKKWVYNALKKNKIQFTMKEPEPDFLEPIRDSSPPDITKGLVA
                     HFPLTSDMKNAVDGKARFTPGSKNKIVNQAYYMDGIYGYTYDQGTISFASDAPEDGYS
                     FSLNVKPEDRKDERQQLLFSGGYRNLDFYRNRKNGQLEVHASSFCKDCGKNKKERWIQ
                     ATYKFSDVNIELNKWHNFTVSMDFKNSRASVLVNGKRLKDINLGADYTDVYKKRIYYR
                     NQVLALDMAGNGSLLKGYVRDMVAYDRSLSGKELLEVQKKYAVNKINVVETENPVPEN
                     KDLPKLNETLLKAAKSGKTDEASAALKEGAEVDTKYKGWTPLLFAAYFGHEETVKELI
                     KNHADPLVEVSGWNAQRFAKAKNYTGIVALLENHSNSSRFFKERKFIVKNSRGDVLPP
                     DLK"
BASE COUNT         1045 a          647 c          739 g         1007 t
ORIGIN      
        1 tgtattcaga gaaatgacgc cgttcgtggc attggaaccg tacagtgcgg aacccggtcc
       61 ccggatgact tccagcgatt tgatcatatt cagaggagtg atttcccaag taagtgcaga
      121 tccgtaaaaa agatcattat gctgtacacc gtctaccagc ataagcagat gattgttgtt
      181 ccagccttcg tacattcctc ttgcagaaac tgttcttctg tcgttgatct gagatgcggc
      241 aaagcctgga agctggtata aaatatcatt aattgatatt cttccgtagt ctctgatctg
      301 tctgtccgaa accacagata cgatgttcgg ggcctcgctt gtttttttgg caaccttggt
      361 tgaggataca gtgacctgct gtccctgcat cagatcgaat acttccgctg cagccttttc
      421 agcagctttt tcctcagtct gcataaaagg cttgagtttt tccgaaagcc tcgatgaatt
      481 tacgtactgt tcaatattgg atatgttttc cttccttcca gggtttgatc tcagaacaaa
      541 ggaagttttt gctgccagtt ttgagatgac tgttctgtcc gtttctctga gttcctcttt
      601 gtccagattc agtccttccg actgaaacac ttcatctgtg acgctgtatg catcaatgat
      661 ctttcctgtc tccggattgt agagctgaat gtacagattc agatttgaat ccggattttt
      721 tttggaataa aagccttcaa tgaggagata tgccttggac tgttttgcgg cagcaagtct
      781 ttccgaaaga gaaattcctt caggactctg tacagtgaag cctgaattcc tgattttctc
      841 tgaaaggatg gaatgaattt ctttttctgt ttccggattt ttttcggatt caaacggctg
      901 aaaactgctg aggatgatat tctcctgtga ataaagattc agagcaggga aaaagacgaa
      961 aagaaaaaaa acagtcccta tgcaatattt catttaatac tccagttttc ttcagacaga
     1021 acgctcatcg gttttttcca aacgaaacag ccagttcact gaagtttttt cagatctttg
     1081 agtctgacag tcagacacat atttcctgtg ttttttattc tgtccaattc ttattgaccc
     1141 gaaaaaataa aaggaccgta tttgtttcat tttgggccaa aatgtcgtat tcagaaattt
     1201 gttcagagcc ggagagtttt ctgaaatgtg gataccgaat ctaaatggaa gtatccttgt
     1261 atttcatatg gaatgacttg cacagatcct gggaggcagg aaatcaccaa aatggcggta
     1321 tcagcagata aaagcagctt agcagcttct aagtttgcgg cagcttctaa gtttgcggca
     1381 gcttctaagt ttgcaaaacc gccccacctg aagccgtagg tgagttactt atcagaatta
     1441 ctgcagagca taaaacctct gaaacaatgc ctgttcggaa tagatcgcat cccttcggtc
     1501 tgcttttaat tgtgttacag aactgctgta aaataattta tctaaaagtt tttgattttt
     1561 cttttcagtt tcttcagtct aagatgaact ggtttttcag tccgacaatg aattaaacga
     1621 ggtcattatg aagatttttc ttctcctgtt tctgaatttt ctctttttga gcagtactta
     1681 cgctgcagaa aagattacaa aagcgaatct gctttccgca agtgcgtcgc ctattctcat
     1741 tataacatct ttaggatgcg atatggggca ggggtatgtc atggcacatg agtccaatct
     1801 ggacgatctt aagggagtaa aaatcggctg ggctgatatc aatgatattt accaaaatcg
     1861 cctgtttgaa tctgcaggtt cggtggaaat tggagatatt cttctagtaa aagacggaaa
     1921 aattctggat acatcgaagg atgcagatgc cgaggacagc attatcgcag gaaatattgc
     1981 aaaaaaatgg gtatataatg cattgaaaaa aaataaaatt cagttcacaa tgaaagagcc
     2041 tgaaccggat tttctggaac cgatcaggga cagctcgcct ccggacatta caaaaggact
     2101 tgtcgcacat tttccgctca cttcagatat gaaaaacgcc gttgacggca aggcaagatt
     2161 tacgcctgga agcaagaata aaattgtaaa tcaggcatat tatatggatg gtatatatgg
     2221 gtatacttat gatcagggaa caatcagttt tgcttctgat gctcccgaag acggatattc
     2281 attttcactg aatgtcaaac ccgaagacag aaaggatgaa cgtcagcagc ttcttttcag
     2341 cggaggatac agaaacctgg atttttacag aaacagaaaa aacggacagc ttgaggttca
     2401 tgcatcttca ttctgcaaag attgcggaaa gaacaaaaag gagagatgga ttcaggcgac
     2461 gtacaaattt tctgatgtaa acatcgaact gaataaatgg cacaatttta ctgtcagtat
     2521 ggattttaag aacagcagag catcagtgct tgtaaacgga aaaaggctga aggacataaa
     2581 tctaggcgca gattatacgg atgtttataa aaagagaatt tattacagaa atcaggttct
     2641 tgcactggat atggccggga acggatctct tctgaagggt tatgtcagag atatggttgc
     2701 atacgacaga tcgctttcag gaaaggaact tttggaagtg cagaaaaaat atgcagtgaa
     2761 taagataaat gttgttgaaa cagaaaatcc tgttcccgaa aacaaggatc tgccaaaact
     2821 gaacgagacc cttttaaaag cagcaaaatc aggcaagaca gatgaagcat ctgcggcact
     2881 gaaagagggc gcagaggtag acacaaagta caagggatgg actcctcttc ttttcgcggc
     2941 atattttggt catgaagaaa cagtaaaaga gcttatcaaa aatcatgccg atcctctggt
     3001 agaagtttcc ggctggaatg cacagagatt tgcaaaggcc aaaaattaca ccggaattgt
     3061 cgctcttctg gagaatcaca gcaattccag caggtttttc aaagagagaa agtttattgt
     3121 aaaaaattcc agaggagatg tacttcctcc tgatctgaag tagtctttag aaaaaatatt
     3181 ttcaaggggc tgtgtaaaag cataaaaaac tgagcttttg cacgcccctc gtaccggttt
     3241 ttgaagtctg caatcagtca gaacaatctc atcctgcatc gttatactga ggatgagacg
     3301 cagaattata tcaggaaatt ctgaattctg taaataaatt tctcattttg atctgagggt
     3361 tttcttgaca gacttgaaaa cttccttaaa cttagaaatg aatagaaatg tttagagaat
     3421 aagttctaat tcaatatt
//
LOCUS       DAOQGJ010000176         3437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15836_length_3437_cov_2.842401, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000176 DAOQGJ010000000
VERSION     DAOQGJ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15836_length_3437_cov_2.842401"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..736)
                     /locus_tag="PK683_03945"
     CDS             complement(23..736)
                     /locus_tag="PK683_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07628.1"
                     /translation="MIYFAIVLILAGIAFFLYAYYEPAKPKKESSVRPERKMKASYRQ
                     MNEEETVIPVNLKATLLNRRYPEHKFSQEVENKIRQERVLSAKFHFPEIQDAVIPERD
                     STDEEEPKKSVSLQNQVKENSGAYSNEIFRLNGTLYFDYSRKIPFTDRKIREKVWDEA
                     DFTHFSRIGKVKMLEIDGVLQFRSGQIYHEFSLEQIEEIVFYEKAFSLIPANPSLPST
                     LVFSEESESFKNLLSVKNI"
     gene            complement(785..2608)
                     /gene="uvrC"
                     /locus_tag="PK683_03950"
     CDS             complement(785..2608)
                     /gene="uvrC"
                     /locus_tag="PK683_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNH07629.1"
                     /translation="MNPEKLKILKNKIRNLSGQSGCYLWKNMEDEVIYVGKALDLSDR
                     VSSYLSSSITDLKTLSLQKEISDLDWIITGSEEEALILEATLIKKYSPKYNIRLKDDK
                     KYPYLCISVNEKFPMIYLTRNVKSDGKKYFGPFTEVKSARDLLSLVHRIFPIRKTVQT
                     LPLKKPGRPCLNFHIKRCLGPCTGNVPEQEYNEIISQIIRFLEGKKSELIIDLQKRMQ
                     ELSEEMKFERAAIYRDMIEALQAVQRRQTVVRISGGDEDILSLAKKDDHGQMVVFEVR
                     SGRLEGKKSFAMSGMLSEKDEDIFLAFLKLYYLNTDYIPEKIMIPLKPDSSLTEFTEI
                     LGRKKDIRIKIRDSRNGSSRGISRLALKNAEMNLTERLLAVKMREEKEALSDLMKMLK
                     LKSEPSVIECYDISHFQGTEPVGSGVMFVDGNPYKPGYRHYKIKSYTGINDPGMIHEV
                     IARRLQRLLNENESLPDLIVIDGGYTQLLRATEAAVALDLPDLPMIGLAKKREEIYVP
                     GEKIPYRFDINSPGLRLLQRLRNEAHRFGVTYHRQRRNEKTLKSALQDIPDIGPARKK
                     EILKYFSGKKKVENADLEELKRVPGIGEKLAEKIFESLHRN"
     gene            complement(2598..>3437)
                     /locus_tag="PK683_03955"
     CDS             complement(2598..>3437)
                     /locus_tag="PK683_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNH07630.1"
                     /translation="DNMYYLNGKSENRDDFVISLSSVALKNGFSLYETLFILKGKPVF
                     IREHLDRLKHSMEYFSLSAEPGFFENIRKDVKKLISETDLLSGRIRIMIFPSKNITLP
                     ADTVLLPEKLLSEEALISLKLSKVRKPFPPQFPSFVKISSNHCSLLSFKEAYESGFDE
                     GLMLDSEGRVTETSYGNLFWIKNNEIFTPSADLSILNGVTRMKIIEICRISGLKLCEG
                     FFSLSEILNSDSAYVSSSSRGMMRISRIDDTIFNEHSSEAVRLIRKKYDEAVENSLNE
                     YEP"
BASE COUNT          844 a          756 c          690 g         1147 t
ORIGIN      
        1 atgttcgaaa aaatttcgga gatcagatat tttttactga aagaagattc ttgaagcttt
       61 ccgattcttc gctgaataca agggtggaag gaaggctggg atttgcaggg atgagactga
      121 atgctttttc ataaaaaacg atttcttcta tctgttctaa tgaaaattca tgatagatct
      181 gtccggaacg gaactggagc acaccgtcaa tctccagcat cttcactttg cctattctgg
      241 agaaatgagt aaaatccgct tcatcccata ctttttccct gatttttctg tcagtgaaag
      301 gtatctttct ggaataatca aaatacagtg ttccgttcag tctgaatatt tcatttgaat
      361 aggcccctga attttctttc acctggttct gcagcgaaac tgattttttc ggttcttcct
      421 cgtctgtact gtccctttca ggaattacag catcctggat ttccgggaaa tgaaattttg
      481 cagagagaac tctttcctgc cggattttgt tttccacttc ctgcgagaac ttgtgttccg
      541 gatatcttct gttcagaaga gtggctttca gatttaccgg aatcactgtt tcttcttcat
      601 tcatctgtct gtatgaggct ttcatttttc tttcaggccg gacagatgat tcctttttcg
      661 gttttgcagg ttcataatac gcataaagga aaaaggctat ccctgcgaga atcagcacta
      721 tcgcaaaata aatcattctg tttatattat ctgtctgatt ccgttttttt tacagaaaaa
      781 aacctcagtt tctgtgcagt gattcgaata ttttctcagc cagtttttct ccgattcccg
      841 gcactctctt aagttcctcc agatctgcat tctctacttt cttctttccg gagaaatact
      901 tcagaatttc ttttttcctc gcaggaccga tgtccggaat gtcctgaagt gccgatttca
      961 gggttttttc attccttctc tgtctgtgat aggtgacccc gaatctgtgc gcctcattcc
     1021 gaagcctctg cagaaggcgg agaccggggg aattgatgtc gaatctgtag ggaatttttt
     1081 ctcccggaac gtaaatctcc tctctttttt tggcaagtcc gatcatcggg agatccggca
     1141 gatccagtgc cacagcggct tccgttgccc gaagaagctg agtatagccc ccgtcaatga
     1201 ctatcagatc agggagggac tcgttttcat tcagaagtct ctgcagtctt ctggctatca
     1261 cttcgtgaat cattccagga tcattgatcc cggtatagga tttgatttta taatgtctgt
     1321 atcccggttt gtaagggttt ccgtcaacga acatcactcc gcttccgaca ggttcagttc
     1381 cctgaaaatg gctgatatcg taacactcaa tcactgaggg ctccgactta agcttcagca
     1441 tcttcatcag atcggaaagc gcttcttttt cctctctcat ttttactgcg agaagtcttt
     1501 ctgtgaggtt catttccgca tttttgagtg cgagcctgga tatccctctc gatgatccgt
     1561 ttctcgaatc cctgatcttt atccggatgt cctttttcct tcctagtatt tcagtaaatt
     1621 cagtgagact gctgtccggt ttcagaggga tcattatctt ttcaggtata taatctgtat
     1681 ttagataata aagtttcaga aaggcaagaa atatatcttc atccttttct gaaagcattc
     1741 cgctcattgc gaacgacttc ttgccttcaa gccgtcccga cctgacttca aatacaacca
     1801 tctggccatg atcgtctttt tttgccaggg acaggatatc ttcgtctccc ccggaaattc
     1861 tgacgactgt ctgccttctc tgaactgcct gaagcgcttc tatcatatcc cggtatatgg
     1921 ccgctctttc aaatttcatt tcttctgaaa gttcctgcat tcttttctga agatcaatga
     1981 tgagttcaga ctttttccct tccagaaacc ggataatctg agatatgatt tcattatatt
     2041 cctgttcagg cacatttcct gtgcagggtc cgagacatct tttgatgtga aaattcagac
     2101 agggtcttcc gggttttttc agcgggagag tctgaactgt ttttcggatg ggaaatattc
     2161 tgtggacgag actcagcaga tccctggcag acttcacttc cgtaaaaggt ccgaagtatt
     2221 ttttcccgtc ggattttaca ttccttgtaa gatagatcat cggaaatttt tcatttacgg
     2281 aaatgcagag atacgggtat ttcttatcat ccttgagcct gatattgtat ttaggactgt
     2341 atttttttat cagtgtggcc tcaagaatca gtgcttcttc ttccgagcct gtgatgatcc
     2401 agtccagatc agaaatttct ttctgcaggg acagtgtctt cagatcagta atcgacgagc
     2461 ttaaatacga actgactctg tcagaaagat ccagagcctt gcctacataa atgacctcat
     2521 cctccatatt tttccagaga taacatcctg actgccctga aagatttctg atcttgtttt
     2581 tgagaatttt cagtttctca gggttcatat tcattcagtg aattttcaac ggcttcgtcg
     2641 tatttttttc tgatcagacg gactgcttct gaactgtgct cattgaaaat cgtatcgtct
     2701 attcttgaga ttctcatcat tccccggctt gaactcgaaa cgtaagcgga atcagaattc
     2761 agtatttcgg aaagtgaaaa aaatccttca cagagtttca atcctgaaat tctgcatatt
     2821 tcaatgattt tcattcttgt cactccgttc aggatgctaa ggtcggcaga cggggtgaat
     2881 atttcattat tttttatcca gaaaagattc ccgtacgagg tttctgtgac acgtccctct
     2941 gaatcaagca tcagtccttc gtcaaatccg gattcataag cttccttgaa actgaggagc
     3001 gagcagtgat tggaactgat ttttacaaat gaagggaact ggggcggaaa gggctttctt
     3061 actttcgaga gtttcagtga gattaatgcc tcttcagaca gaagtttttc cggaaggaga
     3121 acagtgtctg cgggaagtgt aatatttttt gatggaaata tcataatcct gattctgccg
     3181 gacagcaggt cagtctcgga aataagtttt ttcacatctt tccggatatt ttcaaaaaag
     3241 cccggttctg cagaaagact gaagtattcc atcgagtgtt tcagacggtc cagatgttcc
     3301 cggatgaaaa caggctttcc tttcagtatg aaaagggttt catacagact gaatccgttc
     3361 ttcagcgcaa ctgagctgag tgaaatgaca aaatcatccc tgttttccga ttttccgttc
     3421 aaataataca tattatc
//
LOCUS       DAOQGJ010000177         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_15980_length_3418_cov_2.659530, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000177 DAOQGJ010000000
VERSION     DAOQGJ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15980_length_3418_cov_2.659530"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..686)
                     /gene="gmk"
                     /locus_tag="PK683_03960"
     CDS             complement(132..686)
                     /gene="gmk"
                     /locus_tag="PK683_03960"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNH07631.1"
                     /translation="MKGNLYIISSVAGGGKSTLISRILNKHPEILFSVSCTSRPIRPG
                     EKDGVNYHFKSRSEFEELISKDYFYEWALVHDNYYGTPKDFILSNLKQNRKIILDTDV
                     QGAEKVKEQLPESVSIFILPPSIEIWQERLRNRGTETAEVIEKRIRNGFKELKEQDKF
                     DFRVINDSLENAGETLEKILLGNS"
     gene            complement(690..1523)
                     /locus_tag="PK683_03965"
     CDS             complement(690..1523)
                     /locus_tag="PK683_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07632.1"
                     /translation="MELLALVLINVVFGIVLYYVISIKVASSMRDYQFQKVKKEIQVY
                     SLNFFKEAENYLSLMDAKIQILKNLLERAERFDEVSTENLKENLKNEIQDKKRKIEEQ
                     LAEKKLFVNDNIYKTDSGTSDKSDKRKEDSSFSILASLGRSLQSIMGVHPVKTETDAE
                     TASFSKSIPVQSRNTLNISVGGNPFNEYAKIKDENEEDTFEDFLSQPDKNEKRTDDSI
                     VISVKSALQELPEDAGKVDKVVHLLKRGFSHADISDELGMAIPEIALIETIKIEKGRR
                     I"
     gene            complement(1524..>3418)
                     /locus_tag="PK683_03970"
     CDS             complement(1524..>3418)
                     /locus_tag="PK683_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNH07633.1"
                     /translation="EVLKRGKKSLFFSEPFHILVFFSDPDSSLNDLRELDEVLTGGTG
                     RLLDKDLKNLVEPKDLDGYFRIKNYRHGVYLSVFPPVGAGKFVKFEDVIKKFNFRGIP
                     VPPENRIREVIQEAKGEYERLADSKLKQFSEATIKVEISQDHMKAFATFTPPKSGGRD
                     LEVQDVVYELKRSDIAYGIKEEEIRKALDKEIYNEPITVAYGDPAQKGADAKIIYHVR
                     TEKKIRLKEDSFGRVDYKNLDLIENVVSNQLLAEKIPLEKGKHGRDLYNRLLEAKDGM
                     DVLLQPGKGTILSEDGMKLSAEVNGQVIFLDGRVNVETVYRVNGDVNTKTGNITFLGS
                     IVITGNIEDNFHVKASGNIEIFGTVQKAVVEADGDIVIRQGVTGREEARIITTNGNII
                     ARFLQDVEVYTEKDVIVQEMILNSQVNAGGKVICEGKKAQILGGKIRAGSMVLAKNMG
                     SKANPLTEIIVGYSPKLLKQLEDYENKKKESQQKLDDLVKTFKTLVARKEADPASFTE
                     EQETYKQKLDEGVKKLKRRIENFDKEIQTIQSYMDQTAQQGKVYIEKKMYSGVRITIK
                     NSEPYSSKNEINSKIFYLENDKIRQKPYVHPDNEGLDPKKRRNITKKPSGQEDGAEAK
                     EEGSEE"
BASE COUNT          799 a          771 c          620 g         1228 t
ORIGIN      
        1 tatgacatct gtgaagggac tgttctattt tttttcagct ctgttttgga aattggattg
       61 ttccctcagt cccaggtaag tcagtttcgg cagtttccgc aattcgcagt gcagtcagat
      121 tgccgcgttt cttaagaatt tcccaaaaga attttttcca aggtttcgcc tgcattctca
      181 aggctgtcat tgatgactct gaagtcaaat ttgtcctgtt ctttgagttc cttgaagccg
      241 tttcgaatcc gtttttctat tacctcggct gtttctgttc ctctgttccg aagtctttcc
      301 tgccatattt ctatactggg cggaagaata aaaatggaga ctgattccgg aagctgctcc
      361 tttacctttt cggctccctg aacatcggta tccagaatga tttttctgtt ctgtttcaga
      421 tttgaaagga tgaagtcctt cggtgtacca taataattgt cgtgaacaag agcccattca
      481 taaaaataat ctttggagat cagttcttca aattcggatc tggatttaaa atgatagttg
      541 actccgtctt tttctcccgg ccgaatcggt cttgatgtgc aggaaacaga aaacagaatc
      601 tcgggatgct tattgagaat tctgctgata agagtggatt ttccgcctcc tgcgacggat
      661 gaaatgatat acagatttcc tttcaaattt cagattctcc tgcccttttc aatttttatc
      721 gtttcaatca gcgcaatttc aggaattgcc attccaagtt catcagaaat atctgcatga
      781 ctgaatcccc ttttcagcag atgaacgacc ttgtccactt tgcctgcatc ctcaggcagt
      841 tcctgcagag cagatttgac ggatatgaca atgctgtcat cagttctttt ttcattttta
      901 tcaggctgag acagaaaatc ttcaaaagta tcttcttcgt tttcgtcctt gattttagca
      961 tattcgttga acggattccc tccgaccgaa atattcagtg tatttctgga ctgaacagga
     1021 attgattttg aaaaggatgc agtttcggca tcagtttctg tctttacggg atgaactccc
     1081 ataatgctct gaaggcttct tcccagactg gctagaattg aaaaagaaga atcttccttt
     1141 cttttatccg acttatctga ggttcccgag tctgtcttgt atatattatc attaacaaat
     1201 aatttttttt ctgcgagctg ttcttctatt tttctttttt tgtcctgaat ttcatttttc
     1261 agattttctt tcagattctc tgtgctgacc tcatcaaatc tttctgctct ctccagcaga
     1321 tttttcaata tctgaatttt tgcgtccata agagaaagat agttttcagc ttccttaaaa
     1381 aaattcagtg aataaacctg aatttccttt ttgactttct ggaattggta atccctcata
     1441 ctgcttgcca cttttatgga gattacatag taaagtacaa ttccgaatac tacgttgatg
     1501 agaacaaggg cgaggagttc caattattct tccgatcctt cttcttttgc ttctgcccca
     1561 tcttcctgcc cggatggttt tttggtaatg ttccttctct ttttaggatc cagaccctca
     1621 ttgtcgggat gaacatacgg cttctgtctg attttatcat tttccagata gaagatcttg
     1681 gaattgattt catttttgga agagtagggt tctgaattct tgattgtgat tcttacaccg
     1741 gaatacattt tcttttcgat atataccttt ccctgctgtg cagtctgatc catataagac
     1801 tggatagtct gaatctcctt gtcgaaattt tcgattctgc gcttcagttt tttgacacct
     1861 tcatccagtt tctgcttgta tgtttcctgc tcttcggtga atgatgcagg atctgcttcc
     1921 ttccttgcta caagtgtttt gaaggttttg accagatcat cgagtttctg ctgtgattcc
     1981 ttttttttgt tttcatagtc ttccagctgt ttcagaagct ttggactgta tcctacaatg
     2041 atttccgtca gagggtttgc tttggaaccc atatttttag cgagaaccat acttcctgcc
     2101 ctgatttttc cacctagaat ctgtgccttc tttccttcac agatgacttt tccgccggca
     2161 ttcacctggg aattcagaat catttcctga acgattacat ctttctctgt gtatacttcc
     2221 acatcctgaa gaaatcttgc gatgatattt ccgttcgtag tgatgattct tgcctcttcc
     2281 cggcctgtaa cgccctgacg tatgactata tctccgtctg cttctacaac tgccttctga
     2341 actgtaccga atatttctat attgcccgat gcctttacat ggaaattgtc ttctatattt
     2401 cctgtaatta caatggagcc aaggaaggtg atatttccag tctttgtatt tacatccccg
     2461 tttaccctgt acacggtttc cacattgacc cgtccatcca ggaagatgac ctgaccgttt
     2521 acttcggcag aaagtttcat tccgtcctct gacagaattg tgccttttcc aggctgaagc
     2581 agtacatcca ttccatcctt tgcttccaga aggcggttgt aaagatccct tccgtgtttt
     2641 cctttttcca aaggaatttt ttctgcaagg agctgattgg atacaacgtt ttctatgagg
     2701 tccagattct tgtaatctac acgtccaaaa gagtcctctt tgagtctgat cttcttttcc
     2761 gttcttacgt ggtagataat ctttgcatcg gctccctttt gtgcaggatc tccgtatgcc
     2821 acagtgatag gttcattata aatttcttta tcgagagcct ttctgatttc ttcctctttg
     2881 atcccgtatg cgatatctga acgtttcagt tcatacacta cgtcctgcac ttccaagtcg
     2941 cgccctcctg attttggagg agtgaaagtt gcgaatgcct tcatatgatc ctgagaaatc
     3001 tccactttga tcgtggcttc agagaactgc ttcagttttg aatctgcaag acgttcatat
     3061 tctcctttcg cttcctgaat gacctcacga attctatttt caggaggaac tggaattcct
     3121 ctgaaattga attttttgat cacatcttca aattttacaa acttgcccgc tccgactgga
     3181 ggaaaaacag aaagatatac cccatgcctg tagtttttga ttcgaaaata tccgtccaga
     3241 tctttcggtt caaccagatt tttcaaatcc ttgtccagaa gccttccggt tccgcctgta
     3301 agaacttcat ccaattctct cagatcattc agactggaat ccggatcgga gaaaaaaact
     3361 aatatatgaa agggttcact gaaaaacaga cttttctttc cccttttcag aacctcat
//
LOCUS       DAOQGJ010000178         3406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16073_length_3406_cov_2.554461, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000178 DAOQGJ010000000
VERSION     DAOQGJ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16073_length_3406_cov_2.554461"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PK683_03975"
     CDS             <1..742
                     /locus_tag="PK683_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH07634.1"
                     /translation="VRLRAFGSFGFRVSDVEKLFKEISGTNSDFSIDDLDDALKSLVI
                     TEFTDALGELKIPALDLAANYKELSSKIAVFLNEGFKEMGLEVAKFLIENISMPPEVE
                     AAIDKRSQMNVLGNLDQYAKFQAANAMENLSLNTGEGSGMMSGGMGIGMGIGMANMVT
                     NSLNSGQNQQQTPPPLPNQVAFFVSINGAQQGPWNLDQMKAAVMQNQMNRDTLVWKQG
                     MANWAKAGDVPELAAVFGAVPPPLPPQN"
     gene            complement(771..1511)
                     /locus_tag="PK683_03980"
     CDS             complement(771..1511)
                     /locus_tag="PK683_03980"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNH07635.1"
                     /translation="MIIIAHRGVSGHYPENTMSAFRAAVFNGFWIELDVTFTRDRKIA
                     VIHDDTLDRTTNGIGPVNESDYKFIHRLSAGYIFGRSFPNERVPLLQDVCRLISENPN
                     VSMNIEIKSSAFETDFSREGIEKKVLDTVYEFNIKKNTIISSFHPGILSRIRKNDQDI
                     RISYLSEKTEKVDQISILNDCEELKVFSLNLPLEEIGSELFLKAREKGIPVFIYTVNT
                     AEQCRKVREAGAEGIFTNYPEEMKKYLK"
     gene            complement(1516..>3406)
                     /locus_tag="PK683_03985"
     CDS             complement(1516..>3406)
                     /locus_tag="PK683_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07636.1"
                     /translation="EYLNQYGMAYLKIGEYDKSFEKLFGKVDADFGAETDARKTSWTE
                     RTDVPYIEFAKGSSWDNAKLEMAGRSFEMRDALKLDLLKLTAQDKRERTIQKAGAYIV
                     SRLYYKKHDNETYINLGKFHSFSTGDEKYRLKYKNDALAANYFKLVFTDGGDPVNVEA
                     TAGLAKMYYNQGNFGKAASYYNKIIEAHPKNPIGHGGLLSTYIEMWRKDRNPQFVLNH
                     HRIVRNDLSIEDDLSLFTLTKLAAFYIDLDPTEIRIKYNVTPEDQVSNMDIDDNILHI
                     LNIAFKKSEKQDGVEVKGDEYAEQYYQRGRYYIEKNESMRALKQFELAANYDPAHYLA
                     IMEMAEHYMRIDNNVEADKLLSNALMRYNAYSKKFGNKEEDETLIRGDVGRIYFNQGK
                     IVYLRSALLNSSDRIDEFPARKVYPEKSLGNMTDIEKERRGELNSAMKFFDEALKANL
                     QDQSKKRELYYYTAWINYMNSEYETALSSLANMGEEDTLTNPNVMLARANSFYYTEQL
                     NASLGNYLKIKEDFEEKEAAISLPVPEESNHQEIYQTLIAVYNNIGAIYERKGNTAQA
                     LKHYWKSIETARKINTVTEIANYNKDLSFKGTSDKIPLLDDWLSPTVDTIRDLKKSKR
                     KNAWL"
BASE COUNT          878 a          753 c          689 g         1086 t
ORIGIN      
        1 tgtccgtctg cgtgcattcg gttctttcgg attcagagtg tctgatgtag aaaaactgtt
       61 caaggaaatc tcaggaacaa actcggattt ctccattgat gatctggacg atgcactcaa
      121 aagtctcgtc atcacagaat ttacagatgc actgggtgaa ttgaaaattc ctgcactgga
      181 tcttgcagca aattacaaag aactgagttc caagattgct gtattcctga atgaaggatt
      241 caaggagatg ggtctcgagg ttgcaaagtt tctgatcgaa aacatcagta tgcctcctga
      301 agtggaagcg gcaatcgaca agagaagtca gatgaacgtt cttggaaatc tggatcagta
      361 tgcaaaattc caggcagcaa atgcaatgga aaatctgtca ctgaatactg gagaaggcag
      421 cggtatgatg agcggcggta tgggaatagg tatgggaatc ggtatggcca atatggtgac
      481 caattctctg aactcaggcc agaaccagca gcagactccc ccacctctcc caaatcaggt
      541 ggctttcttt gtatcgatca acggtgcaca gcagggacct tggaatcttg atcagatgaa
      601 agctgctgta atgcagaatc agatgaacag agatacattg gtatggaagc agggaatggc
      661 aaactgggcg aaagcaggcg atgttcctga acttgcagca gttttcggtg cagttcctcc
      721 tcctctgccg cctcaaaact aaacttgtct gaggctgtgt aaaagcagcc tcacttcaga
      781 tattttttca tttcttcagg gtaatttgta aagattcctt ctgctccagc ttctctgact
      841 ttcctgcatt gttctgccgt gtttacagta tagataaata ccgggatgcc tttttctcgt
      901 gctttcagga aaagctctga accgatctct tccagtggaa gattcaggct gaacactttc
      961 agctcttcac agtcattcaa aatgcttatt tggtcaactt tctcagtctt ttctgaaaga
     1021 taggatattc tgatgtcctg atcatttttc ctgattctgc tcagtattcc gggatgaaac
     1081 gaggaaataa ttgtattctt ttttatatta aactcatata cagtatcgag gacttttttc
     1141 tctatgccct ctctgctgaa atctgtttca aaagcggagg atttgatttc aatattcatt
     1201 gaaacattcg ggttttctga aatcagcctg cagacatcct gaagaagagg gactctctca
     1261 ttcggaaaac ttctgccgaa aatatagcct gcactcagtc tgtgaatgaa cttgtagtcc
     1321 gattcattta caggacctat tccgtttgta gtcctgtcca aagtatcatc atgaatgaca
     1381 gcaatctttc tgtcacgggt gaaagtgaca tcaagttcaa tccagaaacc gttgaataca
     1441 gccgcacgga aagcggacat tgtattttcc ggataatgac cgctgactcc tctgtgagca
     1501 atgatgatca cgcgattaca gccaggcgtt cttcctcttg cttttcttca gatcgcggat
     1561 agtgtccaca gtcggagaaa gccagtcatc aagaagaggt attttgtctg aagtcccctt
     1621 gaatgaaaga tccttattgt aatttgctat ctctgtaaca gtattgatct ttctggccgt
     1681 ttcaatcgac ttccaatagt gtttcaatgc ctgtgcagta ttgccttttc tttcgtaaat
     1741 tgctccaatg ttattgtata cagcaatcag ggtctgataa atttcctgat ggttggactc
     1801 ttcaggaacg ggaagagata tcgcagcttc tttctcttca aaatcttcct ttatcttcag
     1861 ataattccct agacttgcat tcagctgttc tgtataataa aatgaatttg cacgggcgag
     1921 catcacattc ggatttgtca gtgtatcctc ttcacccata tttgccagac tggaaagagc
     1981 ggtctcatac tctgaattca tatagttaat ccacgcagta taataataaa gctctctttt
     2041 cttggactga tcctgaaggt ttgccttcaa tgcttcatca aagaatttca ttgcactgtt
     2101 cagctcgcct cttctttcct tttcaatgtc tgtcatattt ccaaggcttt tttcagggta
     2161 aacctttctt gcaggaaact cgtctattcg gtcggaactg ttcagaagcg ctgatcgtaa
     2221 atagacaatc ttgccctgat tgaagtaaat tctgcctaca tctcctctga tcagagtttc
     2281 atcttcttcc ttgtttccga atttcttgct gtaggcattg tagcgcatca gtgcattgct
     2341 cagaagcttg tccgcctcca cattattgtc tatgcgcata taatgttcag ccatctccat
     2401 aatggcgaga taatgtgcag ggtcataatt ggcagcaagc tcaaactgct tcagtgctct
     2461 catagattca tttttttcta tgtagtatct gcctctctga tagtactgct ccgcatattc
     2521 gtcacccttg acttccacac cgtcctgttt ttcagacttt ttaaaggcaa tattcagtat
     2581 atgcagaata ttgtcatcaa tgtccatatt cgaaacctga tcctctggag tgacattgta
     2641 tttgattctg atctcagtcg ggtccagatc aatatagaat gcagccagtt tggtcagcgt
     2701 aaaaagtgac agatcatctt caatgctcag atcatttctg acaatacgat gatgattgag
     2761 tacgaactga gggtttctgt cctttctcca catttctatg taagtggaga ggagtcctcc
     2821 gtgtccaatc ggattcttcg gatgtgcttc aatgatctta ttgtaataag aggccgcttt
     2881 cccgaaattc ccctgattat agtacatctt tgcgagacct gcagtggctt ccacattcac
     2941 tgggtctccg ccgtcagtaa atacaagctt gaaatagttt gcagcgagag cgtcattctt
     3001 atatttcagt ctgtattttt catcaccagt actgaaggaa tgaaatttgc ccagatttat
     3061 ataagtttca ttgtcgtgtt ttttataata caacctggat acaatatatg cacctgcttt
     3121 ctgaattgtc ctctctctct tgtcctgtgc tgtcagtttg agcagatcca gtttcagagc
     3181 gtctctcatt tcaaaacttc ttccggccat ttccagcttt gcattgtccc aggaagaacc
     3241 ctttgcaaat tcaatatacg gaacatcagt tctttccgtc caggaggtct ttctggcatc
     3301 agtctctgca ccgaaatcag catctacctt tccgaaaagt ttttcaaatg atttgtcata
     3361 ttcgccgatc ttcaggtagg ccattccgta ctggttgaga tattcc
//
LOCUS       DAOQGJ010000179         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16080_length_3405_cov_2.524776, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000179 DAOQGJ010000000
VERSION     DAOQGJ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16080_length_3405_cov_2.524776"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PK683_03990"
     CDS             complement(<1..481)
                     /locus_tag="PK683_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HNH07637.1"
                     /translation="MYYALISIPLLIIGSVTYFIVNRYRRCPSNKILVIYGKVGKNKS
                     ALCIHGGGAFVWPLIQDSQFLKLEPMTIEINLTGALSKQNIRINTPSTFTVGISTDEN
                     LMGNAAERLLGLTEAQIKTQAEDIILGQLRLVIATLEIEEINQNREKFLAQINENVTT
                     "
     gene            complement(513..1046)
                     /locus_tag="PK683_03995"
     CDS             complement(513..1046)
                     /locus_tag="PK683_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07638.1"
                     /translation="MNIETVYLYSGIFGLIILGAKLTLSIFGADHHSADFDSSSSDFG
                     IISLQTVSVYIAGAGWMGYILTQKTGWDSVQNGLAAGLAGLLLAAFEMFLIYKMKGLN
                     QINEFNLEEAVGKTGIVYLTVPENGTGEVQVSFFGSMKNRKAVSENGEKIESFEKIIV
                     TGIDNDVLIVKKHFSGE"
     gene            1447..1785
                     /locus_tag="PK683_04000"
     CDS             1447..1785
                     /locus_tag="PK683_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH07639.1"
                     /translation="MAQNKKAEFRKDSQALSDFAKALSHPARLEILMEIAKKESCICG
                     EIVEVLPLAQSTVSQHLKELKSAGLISGEVEGTKSCYCVNFEELAKNLKKWERFFEKL
                     NEYNKKGDCC"
     gene            1785..2633
                     /gene="arsM"
                     /locus_tag="PK683_04005"
     CDS             1785..2633
                     /gene="arsM"
                     /locus_tag="PK683_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNH07640.1"
                     /translation="METLTDGNSKKEMVKEKYAQIALQDRSENASSCCGAGGCSTDAY
                     TIFNEDYTGLKGYNPDADLALGCGIPVKYAGIKEGDTVVDLGSGAGNDVFVARSLVGD
                     DGRVIGIDMTEVMIEKARKNNEKLGYSNVEFRLGDIEKMPVGPNRADVVLSNCVLNLV
                     PDKEKAFSEIQRILKPGGHFCISDIVMSGVLPEKIQNAAEMYAGCISGAMQKNDYLTV
                     IHNAGFQNVSIRTEKEIQVPDDILLDYLSADELKNLKDSGVKIESITVTAEKAKSECC
                     SSSGCC"
     gene            complement(2853..3365)
                     /locus_tag="PK683_04010"
     CDS             complement(2853..3365)
                     /locus_tag="PK683_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07641.1"
                     /translation="MKIEAFMRITFIFFIITVFPIFSKGKFASSYTDFAKDCKGDPSV
                     RDGDIPQICKGPARFTLYITYSACSEFIRILDKKKKEILSLPEQTIGSSDKKKLEWRI
                     LDGRPIAVIYRISIYEESSDDPCTRKKKKEELGVYGIGKYSDLSKTVSGKNENEKARQ
                     TADEFILGKK"
BASE COUNT          958 a          686 c          759 g         1002 t
ORIGIN      
        1 cagtggtcac attctcattg atctgagcca gaaatttctc acggttctga ttgatctcct
       61 caatttccag agttgctatt acaagacgga gctgacctaa aataatgtct tccgcctgtg
      121 tcttgatctg agcttcagtc agtccaagaa gtctttccgc tgcatttccc atcagatttt
      181 catcagttga tattcctact gtgaatgtgc ttggtgtatt gatccgtata ttctgcttgg
      241 agagggctcc tgtcagattg atttcaatcg tcatcggctc aagtttcaga aactgagaat
      301 cctgaatgag aggccataca aatgcaccgc ctccgtgaat gcagagagca cttttgtttt
      361 ttcctacttt tccgtagatg accagaattt tattggaagg acatcttcta tatctgttta
      421 caataaaata cgttacggaa ccgatgatga gcagagggat gctgatcaga gcatagtaca
      481 tataatctcc tgtgggtttt ttaacggaga gtttattccc cggaaaaatg ctttttaaca
      541 atcagaacat cattgtctat gcctgtcact atgatttttt caaatgattc aatcttttct
      601 ccgttttcag agactgcttt tctgtttttc atacttccga aaaatgaaac ctgcacttct
      661 cctgttccat tttcaggaac agtcagataa acaatccctg tttttccgac tgcttcttcc
      721 agattgaatt cattgatctg attcagtcct ttcattttgt atatgagaaa catctcaaat
      781 gcggccagca gaaggccggc gagtccagca gccagaccgt tctgaacaga atcccatcct
      841 gtcttctgag tcaggatgta tcccatccat cctgcacctg caatataaac ggatactgtc
      901 tgaagagaaa taatgccaaa gtccgatgag gaactgtcga agtcagccga atgatggtct
      961 gctccgaaaa tgctcagtgt cagttttgct ccaagaatga tcagaccgaa aatgcctgag
     1021 taaagataca ctgtttctat gttcattgtt caacagtagt gactgtctgt ctgctgtaaa
     1081 acactattcc ctgctgtcca gaaacaggtt atccaaaacg ccgctttcag gtctgtgagc
     1141 actagagccg ctgtcaagtt aagactattg tcaaaatggc ttttccgaac tgcgttcctt
     1201 aaatcagcat ccttcgatgg gctaaagccg tacagctttg gaagacagac gttccgggta
     1261 tgaactggaa ggcggccgaa ggttcataaa atgaattcag gaaatcaggg aataatattt
     1321 cagaagagaa gaaaacagtg tgacttgacg acgcggcggt atttttctcc ggagcggttt
     1381 atctttgttc gattttttta aaaaactgct tgacgaatat cgtatatcgt atatatacga
     1441 ttatatatgg ctcagaacaa aaaggctgaa ttcaggaaag actcgcaggc tctttctgat
     1501 tttgcaaaag ccctttctca tcctgcaagg cttgagatac tgatggagat cgcaaaaaaa
     1561 gaatcctgca tctgcgggga gatcgtggaa gtcctcccgc tggcacagtc cacggtctca
     1621 cagcatctga aggaactgaa gtcggcagga ctgatttcag gagaagtgga agggacaaaa
     1681 tcatgctact gtgtgaactt tgaagaactt gcaaagaacc tgaaaaaatg ggaacgtttt
     1741 tttgaaaaac tgaatgaata caataaaaaa ggagactgct gctgatggaa actctcacag
     1801 acggaaacag taaaaaagag atggtaaagg aaaaatatgc acagatagcc cttcaggaca
     1861 gatcagagaa tgcatcttcc tgctgcggtg ccggcggatg ttcgacagat gcgtatacaa
     1921 tttttaatga agattataca ggactgaaag gatacaatcc tgatgcggat ctggctctcg
     1981 gatgcggaat ccctgtgaag tatgcaggca tcaaggaagg agacactgta gtggatctgg
     2041 gctccggtgc cgggaatgat gtgtttgttg caagatcact tgtcggggac gacggaagag
     2101 tcattggaat cgacatgacc gaggtgatga tcgaaaaggc aagaaagaat aatgaaaaat
     2161 taggatattc taatgtagaa ttccgtctgg gagacattga aaaaatgcct gtaggaccga
     2221 acagggcgga tgttgtccta agcaactgtg ttctgaatct tgttccggat aaggaaaaag
     2281 ctttctccga gattcaaagg atactgaagc ccggaggaca tttctgcatt tcagacatcg
     2341 tgatgtcagg agttcttccg gaaaaaattc agaatgcagc cgagatgtac gcaggctgta
     2401 tttccggagc aatgcagaag aatgactatc tcacggtcat tcacaatgca ggctttcaga
     2461 atgtgagtat cagaacagaa aaggaaattc aggttcccga tgatatactt ctggattatc
     2521 taagtgctga tgaactgaag aatctgaaag acagcggtgt gaaaatagag agcattactg
     2581 tgactgcaga aaaggcaaaa tcagaatgct gttcttcttc tggatgctgc tgaggcaggg
     2641 cggcgatttc cggcgattag actgtcaagg tcgtttcttc tgatggcagg aatattggct
     2701 cggcatcctg tcggctggat gtgcaataga ttgaaaaaaa ctgtctccat ctttctgctt
     2761 ggagacagtt tttgaaagat gtttatttct gaatcggaaa gatcctggtg tcctgcacga
     2821 tctgcctgtt ttcggaacag gatttactga aattattttt ttccgaggat aaattcatcc
     2881 gctgtctgtc tggccttctc attttcattc ttgcccgata ctgttttcga aagatcggaa
     2941 tactttccta ttccgtaaac tcccagttct tcctttttct ttttgcgcgt gcagggatca
     3001 tctgaacttt cttcgtatat gctgatcctg tagattaccg caattggtct gccgtctaaa
     3061 attctccatt ccagtttctt cttgtctgaa cttcctatgg tctgttcagg cagggagagg
     3121 atttctttct tttttttatc caggattctg atgaattcgg aacatgcgga ataggttata
     3181 tacagggtga atctggcagg tcctttgcat atttgaggaa tgtctccgtc ccttacactc
     3241 ggatcacctt tgcagtcttt tgcaaaatcc gtataggatg aagcaaattt tccttttgaa
     3301 aaaatcggaa atactgtgat aataaaaaaa atgaatgtaa tcctcataaa cgcctctatt
     3361 ttcaatgaat aatcctttta tttttttcct tacacattgt ctgtc
//
LOCUS       DAOQGJ010000180         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16089_length_3403_cov_3.535842, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000180 DAOQGJ010000000
VERSION     DAOQGJ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16089_length_3403_cov_3.535842"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PK683_04015"
     CDS             <1..472
                     /locus_tag="PK683_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNH07642.1"
                     /translation="VLILTGFGFWVSRRARIQDIAASFIFSYMFFLLYRTFYYGYSFS
                     VFLHQISNGSLILFTFFMITDPRTAPDSGKARILHGFLVALLAYNLQFALYMKTSLIW
                     SLFFLSFLVPLWDRIFKGSQYIWNNGFKGDNNEKIFQKDCSADSHPVGVTVRSV"
     gene            402..1760
                     /locus_tag="PK683_04020"
     CDS             402..1760
                     /locus_tag="PK683_04020"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNH07643.1"
                     /translation="MKKFFRKTVLLILILSVSLFVPYKEILAFCGFYVAKADATLYNN
                     ASRVVLVRNEDKTVISMMNDYKGALKDFAIVVPVPVVLQKEQIHVGNPKTVDHIDAYS
                     APRLVEYFDSDPCRRMYDEAEDVKIVPSPSAGNNKDPFKGGKTDHGVTVEATYTVGEY
                     DIQILSAKYSSGLETWLVQNGYKIPQGASKALKPYIQQNLKFFVAKVNLKEHASAKLT
                     YLRPLQFAFNSRQFMLPIRLGMINAQGPQDLLIYVLTKNGRVESSNYRTVKLPTDMEV
                     PSFVKKEFSDFYKSMFDNQTKKENLRAVFTEYFWNMGWCDPCAAEPLSFQELKELGVF
                     WLDEKNSYGGAVPVMVTRLHVRYSNETFPEDLVFQETNDQQNFQGRYIIQHPWTGNRN
                     DCRAAETYFQELEKREDQRAKNLASLTGWDLSSIYKKMNRQSYGNTKSGDGEKWWKKI
                     WK"
     gene            1762..2625
                     /locus_tag="PK683_04025"
     CDS             1762..2625
                     /locus_tag="PK683_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07644.1"
                     /translation="MKYLIIIFCLMLPFSAYSEGMPAIEGADDSLTVCQGNPDKLLMD
                     AVDNDDFSLAKACIKQKANMPAAYEYALRKGKMDFATLFIAKGMYIDKEELLYDAVKI
                     CYAPKEWKEKMSKGKDQCTILNWLISRGADVKKADAKKSFFMEATKFSHGRDDGLLGK
                     EDKSYEEMVRFLIKNGASVNPPPKYDEGEERILVRLAELEAHISIFQILIQSGASLTD
                     RNPKKETVLRSVEKLYYKEKENLRSGEKIPKWQKTEPIHVSLAKRRVPYLEGIIQFLK
                     DKEKENSAKKG"
     gene            2627..>3403
                     /locus_tag="PK683_04030"
     CDS             2627..>3403
                     /locus_tag="PK683_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07645.1"
                     /translation="MMKLLIIYLFCILAAVSEISSESADDGKSFLAADRRTDFYIQAS
                     RVNWTPAAGSQFFRMQETRNLEGALFKGSLPLTSGFHFNTGKAESVQASSIGAGMHIG
                     SKSGTHRGHLNLQFMESSPRSFNSLNYYTSGTAFNSFSANYAELAVQSHSMNSQRRTS
                     LGYDHDFYLLPDHPSRFLTGLGLKVGALMQYDTMSGGYTSFGSYAASSTAASLNTGNS
                     IPVKNRIKFDDYMGLTLIGLVYRLVLTETQEIELGMNYYRG"
BASE COUNT         1014 a          678 c          795 g          916 t
ORIGIN      
        1 cgtactgatt ctcacaggtt tcggattctg ggtttccaga agggccagga tacaggatat
       61 tgcggcctcc ttcatctttt catatatgtt ctttctttta tacagaacat tctattacgg
      121 atattccttc tcagtctttc tgcatcagat ttcaaacgga tctctcattc tgttcacctt
      181 ttttatgatc actgatccga gaactgcacc ggattcagga aaggccagaa ttctccacgg
      241 ctttctcgtc gctcttctcg cctacaatct gcagtttgct ctttatatga agacttcact
      301 gatatggagc cttttctttc tttccttcct tgttcccctc tgggaccgga tcttcaaggg
      361 aagtcaatat atatggaata atggatttaa aggagataat aatgaaaaaa tttttcagaa
      421 agactgttct gctgattctc atcctgtcgg tgtcactgtt cgttccgtat aaggaaattc
      481 ttgctttctg cggtttttat gtagccaagg ctgatgccac gctctacaac aatgcatcaa
      541 gagtagtgct tgtaagaaat gaggacaaaa cggtcatcag catgatgaac gactacaagg
      601 gtgcattgaa ggattttgca atcgttgttc ccgttcctgt cgtgcttcag aaggaacaga
      661 ttcatgtggg gaatccaaag acagtcgatc atatagatgc atattctgcg cccagacttg
      721 ttgaatactt tgattctgac ccctgcagaa gaatgtatga tgaggcagaa gatgtgaaga
      781 ttgtcccttc tccttctgca ggaaacaata aggatccgtt taagggcgga aagacggatc
      841 acggagtgac agtggaagca acatacacag tcggggaata tgacatacag attctgtctg
      901 caaaatattc ctcaggtctt gaaacctggc ttgtgcagaa cggatataaa attccacagg
      961 gtgcatcaaa ggctctcaaa ccctacattc agcagaatct gaaattcttt gttgcaaagg
     1021 tcaatctgaa ggaacacgca tctgcaaagc tcacttatct cagacctctg cagtttgcct
     1081 tcaattcaag acagtttatg cttccgatca gactgggaat gatcaatgca cagggccctc
     1141 aggatctgct gatctacgtt ctgacaaaga atggaagggt tgagtcttcc aattacagga
     1201 cagtgaagct tccaactgat atggaagttc cttcctttgt gaaaaaggaa ttttccgatt
     1261 tctacaagtc gatgttcgac aatcagacga agaaggaaaa tctcagggca gtgtttactg
     1321 agtatttctg gaatatgggc tggtgtgatc cctgtgcagc agagccgctt tcctttcagg
     1381 aactgaaaga acttggggta ttctggctgg atgaaaaaaa ttcatacggc ggggcagtgc
     1441 ctgtcatggt taccagactt catgtacgtt attcgaatga gacttttccg gaagaccttg
     1501 tgttccagga aacaaatgat cagcagaact ttcagggcag atacatcatt cagcatccct
     1561 ggactggaaa cagaaatgac tgcagggcag cggagacgta ttttcaggag cttgaaaaga
     1621 gagaggatca gagagcaaaa aatcttgctt ctctcaccgg atgggatctc agttcaattt
     1681 acaaaaagat gaacaggcag agctacggaa acactaaatc cggagacgga gaaaagtggt
     1741 ggaaaaagat ctggaaatga tatgaaatat ttaatcatta ttttctgttt aatgctgccg
     1801 ttttctgcct attccgaggg aatgcctgca attgaaggcg ctgatgattc tctcacagtc
     1861 tgtcagggca atcctgacaa gctgcttatg gatgctgtag acaacgatga tttttctctt
     1921 gcaaaggcct gcattaaaca gaaggcaaat atgcctgcag cgtacgagta tgcactcagg
     1981 aagggaaaaa tggacttcgc cactctcttt attgcaaagg gaatgtacat agacaaggaa
     2041 gaacttctct atgatgcagt gaaaatctgc tacgctccca aggaatggaa ggaaaaaatg
     2101 tccaaaggaa aggatcagtg tacaatactg aactggctga tttccagagg agcagatgta
     2161 aaaaaggcgg atgcaaaaaa atcctttttt atggaagcca ccaaattttc ccatgggagg
     2221 gatgacggtc ttctcggaaa agaggataaa tcctatgagg aaatggtcag gtttctgata
     2281 aagaacggtg caagtgtaaa tccgcctcca aaatatgatg agggtgagga aagaatcctg
     2341 gtaagactgg cagaactgga agcccatatc agcatttttc aaatactgat tcagagcggg
     2401 gcctcactga cggacagaaa tccgaagaaa gaaacagttc tccgttcagt ggaaaaactc
     2461 tattacaagg aaaaggaaaa cttgagatcc ggagaaaaaa ttccaaaatg gcagaagacg
     2521 gaaccgatcc atgtttctct tgcaaaaaga agagtgcctt acctggaagg aattattcag
     2581 tttttgaaag ataaggaaaa ggagaattca gcaaaaaagg gataatatga tgaagctatt
     2641 gattatatat ttattttgta tattggcggc tgtttccgaa atatcatcgg aaagtgcgga
     2701 tgacggcaag agttttcttg ctgcggacag aagaaccgat ttttacattc aggcctcaag
     2761 ggtaaactgg acgccggcgg cagggagtca gtttttcaga atgcaggaaa ccaggaatct
     2821 ggaaggtgct ctgttcaaag gatcactgcc tctgacaagc ggttttcatt ttaatactgg
     2881 gaaagctgaa tcagtgcagg caagttcgat cggtgcagga atgcacatcg gatcaaagtc
     2941 aggaactcac agaggtcatc tgaatctgca gtttatggaa tcatctccca gatcattcaa
     3001 ttctctgaat tattatacat cgggaactgc cttcaacagc ttttctgcaa actacgctga
     3061 acttgcagta cagtcgcact caatgaattc tcagcgaaga acctctctgg gctatgatca
     3121 cgatttttat cttctgccgg atcatccctc ccgttttctg actggacttg gtctcaaggt
     3181 gggggcactg atgcagtacg atactatgag cgggggatat accagttttg gaagttatgc
     3241 tgcatcttcc acagcagctt cattgaatac ggggaattca attcccgtaa agaacagaat
     3301 caaattcgat gactatatgg gtctgactct gattggactt gtctacagac tggttctgac
     3361 ggaaacacag gaaattgaac tgggaatgaa ttattacaga ggt
//
LOCUS       DAOQGJ010000181         3398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16131_length_3398_cov_3.281484, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000181 DAOQGJ010000000
VERSION     DAOQGJ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16131_length_3398_cov_3.281484"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PK683_04035"
     CDS             <1..1092
                     /locus_tag="PK683_04035"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNH07646.1"
                     /translation="FSYACYYLGRLIHADIVHSHDWHTALVNVCNHFLAEGRPTVFTI
                     HNLAYQGDHPYWMTSFLRVDPFNINTEVLDNLGKVNYMKGALMFANEITTVSPGYREE
                     TLHEPEGYFLSWLLNERRYNYTGILNGIDDRVWNPSFDTKIALNYNSSNAEEGKRENK
                     LSLYKEYGLDIDLDRPLIGMVTRLTHQKGIETFLQSFREKWGLPFYYFILGSGDEKLE
                     NTLFHFSHHDHKRLFFFKGFEDALAHKVEAAADFFLMPSLFEPCGLNQMYSQVYGTVP
                     IVSRVGGLKDTVREDADRHYMTGIVFEKGEAHSLNYALDRANTLFWNKADLSIVRRNI
                     MHIDWGWKGSEMRYTHLYEKIRQVRRRAV"
     gene            1272..>3398
                     /locus_tag="PK683_04040"
     CDS             1272..>3398
                     /locus_tag="PK683_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH07647.1"
                     /translation="MKILSLLRHIFRTVGIADFSLSWKLTVFSILSLLFFLDCSNASL
                     AKSPPAVKGHLYLQDWDFSKGYAVLDGEWEFFRNVFFENSDELKKFTDSEKAKSPDFI
                     SVPSVWNSKADPSAQGEKIPVFGHAAYRLKVHTSKYSRPLGLRITDIGMAYSLYINNI
                     FISSNGKPGRSREEMQSLLKYREIQIPSSETELDIVFLVSNYEYMESGIWSRIKLGHY
                     DDLIKSQKTDTTVDFIVLGSLLIMGFYHLGLFMLRKKEKSPLFFGLFCLLVALRIISV
                     ESRSLLDWISFLSFYEVYRMDFFTFYAGVPVFALFLFHLFHAEFNTFILRTFLILSIP
                     PALLPLTTPISVYPKVLIFVQTITLALAFFTVFILIKAIRRKSTGALIFLFGAVPFIV
                     TIMNDILYANQAIHTFYMGSYGLLVFIFSQSIVLSRRFSKAFSLTEKLASELEAQRES
                     LELSNRELQKLKENLEQIVSERVQELDASKREVEMLNEFSRAVNENLDIGEIMNTVMN
                     YVTPNFGIHNYGLYLTDRNTETVKCLRASFPDHMSEGDRKYVLDTPIGLNARRAIHSR
                     VFRTGTVVYFYRIPKSEVEAENKIAYLYQMRSFISVPLKMKNEVIGILDFFSMNTEMK
                     IDESALNRISVLADQVVGAVNNARLLKETDQAKQLAESEKMRSEKLLLNILPKDVAEE
                     LKDKGTAEPVSFDSVSVLFTDFQGFTK"
BASE COUNT          998 a          639 c          730 g         1031 t
ORIGIN      
        1 ttctcctatg cctgctatta tctgggaaga ctgattcatg cagatatagt tcattcccac
       61 gattggcata cagcacttgt aaatgtctgc aaccatttcc tcgctgaagg aaggccgact
      121 gtgttcacaa ttcataatct tgcctatcag ggggatcatc cttactggat gacgagtttt
      181 ctgagagtgg accctttcaa catcaacacg gaggtcctgg acaatctggg caaagtgaat
      241 tatatgaaag gtgctctgat gtttgcaaat gagattacta cggtcagccc cggatacagg
      301 gaggagactc tgcatgagcc ggaaggttat ttcctttcct ggcttctgaa tgaaagacgt
      361 tataattata ctggaattct gaacggaatt gatgacaggg tctggaatcc ttcatttgac
      421 actaaaatag cattaaatta taattcatca aatgcagaag aggggaaaag ggagaataag
      481 ctgagtcttt ataaggaata tggactggat attgacctcg acagaccttt aatcggtatg
      541 gtgactagac ttactcatca gaaagggata gaaacttttc tgcagagttt ccgtgaaaaa
      601 tggggcctcc ccttttatta tttcatactt ggatcaggag atgagaaact ggaaaacact
      661 ctgtttcatt tttctcatca cgatcataaa cggcttttct tcttcaaggg atttgaagac
      721 gcccttgctc ataaagtgga agcagcagcc gacttttttc tgatgccttc cctttttgaa
      781 ccttgcggac tgaatcagat gtacagccaa gtgtatggga cagttccaat tgtgagcagg
      841 gtaggcgggt tgaaggacac ggtcagggaa gatgcggaca gacattatat gacaggaata
      901 gtctttgaaa aaggggaagc tcattcattg aactatgctc tggacagagc gaatactctt
      961 ttctggaaca aagcagatct gagcatagta agaagaaaca taatgcatat tgactggggc
     1021 tggaaaggaa gcgagatgcg ttatactcat ctttatgaaa agatcaggca ggtcagacgc
     1081 agagctgtat gaaaaactga tgaggaattc aggagagtct gcagagagca ggttctcctg
     1141 atttatgctg atagaacacg tttttttccc aaatctgcaa agttcatatt tttataaaaa
     1201 tcctttttac tgcttttctt aaaagcaatt tatttactta ctggcatatt gagtatagca
     1261 ttacatataa tatgaaaatt ttaagcctgc ttcgacacat attcagaacc gtaggaattg
     1321 cagatttttc tctttcctgg aaactaactg tattcagtat tttgtcatta ctgttttttc
     1381 tggattgcag caatgcttca ctggcaaaaa gtcctcctgc tgtaaagggt catttatatc
     1441 ttcaggattg ggacttttca aaaggttacg ctgtcctgga cggtgaatgg gaatttttca
     1501 ggaatgtctt ttttgaaaat tctgatgagc tgaaaaaatt tactgattcg gaaaaggcca
     1561 aatcccctga ttttatttca gttccctctg tttggaactc taaggcagac ccttctgcgc
     1621 aaggtgaaaa gattccagtt tttggacacg ctgcctaccg cctgaaagtg catacatcaa
     1681 aatattccag acctttagga ctcagaatta ctgatattgg aatggcatac agtttatata
     1741 ttaataatat ttttatatcc tcaaatggaa aaccgggcag aagcagagaa gaaatgcagt
     1801 cccttttgaa atacagggag attcagattc cctcaagtga gacagaactg gatattgtat
     1861 tccttgtttc caattatgaa tatatggaat caggcatctg gagcaggata aaacttgggc
     1921 actatgatga tcttataaaa tcgcaaaaga ccgatacaac ggtcgatttt attgtgcttg
     1981 gcagtcttct gattatgggg ttctatcatt tgggactttt tatgctcaga aaaaaagaaa
     2041 agtcgccttt gttcttcggt ttattctgtc ttctggtcgc tctaaggatc atatccgttg
     2101 agtcaaggtc actgctcgac tggatatcct tcctttcctt ttatgaagtt tacagaatgg
     2161 atttctttac cttctatgca ggtgttccag tatttgcgct tttcctgttt catctttttc
     2221 atgcagaatt caatacattc atcctgcgga cttttcttat actcagcatt cctcctgcac
     2281 ttctccccct gacgactccc atttcggtat atcccaaagt acttattttt gtacaaacta
     2341 tcactctggc ccttgcattt tttacagtct ttattctcat caaggcaatc agaagaaaaa
     2401 gcacaggtgc tctgatcttt ctcttcggtg ctgttccttt catcgttaca attatgaatg
     2461 atatactgta tgccaatcag gcaatccata cattttatat gggcagttac ggtcttctcg
     2521 tatttatctt ttcccagtcc attgttcttt ccagaagatt ttcaaaagca ttctctctta
     2581 cagaaaaact agcttcggaa ctggaggctc agagagaatc gcttgagctg agcaacaggg
     2641 agcttcagaa acttaaagag aacctggagc agattgtcag tgaaagagtt caggagctgg
     2701 atgcttccaa aagagaagtg gaaatgctga atgagttttc gagagcagtg aatgaaaacc
     2761 tggacatcgg tgaaattatg aatacagtga tgaattatgt gacaccgaat tttggaattc
     2821 ataattatgg attatatctg actgacagaa atactgaaac ggtaaaatgt ctgagagcat
     2881 ctttcccgga tcatatgagt gaaggagata ggaaatatgt tctggacaca ccgatcggac
     2941 tgaatgccag aagggctatt cacagccggg tcttcaggac aggaacagtt gtatactttt
     3001 acagaatacc gaaatctgaa gtggaagcgg aaaataaaat tgcttatctt taccagatgc
     3061 gttcattcat ctctgttcca ctgaagatga agaatgaagt gatcggcata ttggattttt
     3121 tcagcatgaa tacggaaatg aaaatagatg agtccgcatt gaacaggatc tccgtacttg
     3181 cagatcaggt agtaggcgcg gtgaacaatg cgagactgct caaggaaact gaccaggcaa
     3241 aacagcttgc agaatcggaa aagatgagat ctgaaaaact tcttctgaat attcttccca
     3301 aggatgttgc tgaagagctt aaggataaag gaactgcaga gcctgtatct tttgattctg
     3361 tatccgtact gtttactgac tttcagggtt ttactaaa
//
LOCUS       DAOQGJ010000182         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16144_length_3396_cov_3.055073, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000182 DAOQGJ010000000
VERSION     DAOQGJ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16144_length_3396_cov_3.055073"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1386
                     /locus_tag="PK683_04045"
     CDS             94..1386
                     /locus_tag="PK683_04045"
                     /inference="COORDINATES: protein motif:HMM:NF009587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNH07648.1"
                     /translation="MTRKIYSNLYFQVILGIAAGITLGFFHPDLGAKMRPLGDGFIRL
                     IKMVISPIIFITVVTGIAKVGSLKSLGRVGLKSLIYFEVISTLALIIGMAVLNVYEPG
                     KGMNVNPAALDTSEILSLQNKAHSTEHRSTVGFILGIIPNQFLGAFTEGSILQVLFIS
                     VLFGVAMAESGAAGERLLEIIEEASTVFFRIISMIMKLAPVGAFGAMAFTVSSFGAKS
                     LIPMLKLMGSLYFTCFLFIFTVLGLIARLAGFSIWKFLKFIRQELLIVLGTSSSESVL
                     PRIMKKLEAAGCGRSVVGLTIPTGYSFNLDGTSIYLTMAALFIAQATNVNLSLEQQIS
                     ILGILLLTSKGAAAVTGAGLVTLAATLSTIETLPVNGIALIIGIDRFMSEARSITNLI
                     GNAVATVVISKWENALDSDRLDKVLSGEIKLPEYDSHH"
     gene            complement(1380..3062)
                     /locus_tag="PK683_04050"
     CDS             complement(1380..3062)
                     /locus_tag="PK683_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH07649.1"
                     /translation="MKYFFMLLLILFTGLSSEERKIVIHTEIFSEEREEQGGWVSAGI
                     QRLVTYGIEQIASVRLSEYEDMKFAYGNVKRQIPRPERERMIRDKTEMDLILKGSVRL
                     NGSDIIVYLEMTNVRSPVRKKSFVSAGSVGKISLLRLILLEKIIQAANETAGPGFVPA
                     SFTEKEKERFLQYPEFSENGFFSYSRALSLRKTNPEESILLFSQALSGDGSIPELLID
                     SAVMLSRKGEYLNDSERLLNRVQEILRKNEEVITFNYIRVYYYLGVVFFNRGEFSTAL
                     EYFLKAKTMSESMNLIHTVGYLEILNYLGDIHMKKGNLNSAVEYWLSCKREYELRKMT
                     DSIYYADLLNNTGVYYLERNSVTGFEFLKTAREIYEKYRYRRKIGYANNLSNFGNYFL
                     SQNSPDTAYYYYARSLKYYERSANTQTPGYANALNNISTIHFRKKEYKESLAFLEKSK
                     SVYEELNLTGTKTYAVILQNMGNLHLALKNPLTSYDYFRSAENVFEGLRLTDSLQYAQ
                     VMEKIGDMELERRGVKEAVYSYERAYTAYLKSKNEKEAAVMKKKYDSLRKTQ"
BASE COUNT          959 a          736 c          676 g         1025 t
ORIGIN      
        1 agttcatgga gatactgact ctattgatta ttttctcctt gtgctctgtg cagaaaaagg
       61 tacttttaca cagacccggc aggagattta aaaatgacac gtaaaattta ctcaaacctc
      121 tactttcagg tgatacttgg aattgcagca gggataacgc tcggattctt tcatccggat
      181 ctgggagcaa aaatgagacc tctcggagac ggattcatca ggctcatcaa gatggtcatt
      241 tctccgatca tattcatcac agttgtgaca gggattgcga aagtaggaag tctgaaatca
      301 ctgggaagag tcggtctgaa atctctgatc tactttgaag tgatctcaac tctggctctc
      361 ataataggaa tggcagtgct gaatgtctat gaacccggaa aaggaatgaa tgtaaatcct
      421 gccgcactgg atacttcaga aattctaagt cttcagaaca aggcacattc aacagaacac
      481 agatccactg tgggttttat tctgggcatc attccaaatc agtttctggg agcattcact
      541 gagggaagca ttctgcaggt gcttttcatt tccgttcttt tcggagtcgc aatggcagag
      601 agcggagccg caggagagag actgctggaa atcattgaag aggcctcaac cgtttttttc
      661 aggattatca gtatgattat gaaattggcc cctgtaggcg cattcggagc aatggccttc
      721 acggtcagtt ccttcggagc aaaaagtctg atccctatgc tgaaactgat ggggagcctg
      781 tatttcacct gttttctgtt catatttact gtgctgggac taattgcaag acttgccgga
      841 ttcagcatat ggaagtttct gaaattcatc cgccaggaac ttcttatagt gcttggaacc
      901 tcctcatccg aatcagttct tccgagaatc atgaaaaagc tggaggccgc aggatgcggc
      961 agatccgtag taggacttac gattcctacc ggctactcat tcaatctgga cggaacgtct
     1021 atttatctga ctatggctgc tcttttcatt gcacaggcga cgaacgtcaa tctttctctt
     1081 gaacagcaga tttccattct ggggattctt ctcctgactt caaaaggcgc tgcagcggtg
     1141 acaggagcag gtcttgttac gcttgctgct accctctcca cgattgaaac ccttcctgta
     1201 aacggaatcg ctctaatcat aggaattgac agattcatgt ccgaagcaag atccattaca
     1261 aacctgatcg gaaatgctgt tgccactgtt gtcatttcca agtgggaaaa tgcactcgat
     1321 tcagacagac tcgataaggt tctttcagga gaaataaaac tgcccgaata tgattctcat
     1381 cactgagttt ttctgagaga atcgtatttt tttttcatta cagcagcctc tttttcattc
     1441 ttcgatttga ggtaagctgt ataggccctc tcatatgaat atacagcctc cttgacccct
     1501 cttctttcca gctccatatc tccaattttt tccatcacct gtgcatactg cagagaatca
     1561 gttagacgga gaccttcgaa gacattctct gcagaacgga aataatcata agaggtaaga
     1621 gggtttttca gagcaagatg caggtttccc atattctgaa gtatcactgc ataagtcttc
     1681 gtgcccgtaa gattaagttc ttcataaaca gatttggatt tttcgagaaa agccagactt
     1741 tccttgtact ctttttttct gaaatgtatt gtcgaaatat tgttcagtgc atttgcatac
     1801 cctggggtct gtgtattagc tgaacgttca taatatttta atgaccttgc atagtaataa
     1861 taggctgtat caggactgtt ctgcgaaaga aaataatttc cgaaattgct cagattgttt
     1921 gcatatccga tttttcttct gtatctgtat ttttcataaa tctctctggc agttttcaga
     1981 aactcaaaac cggtaacgga atttctttca aggtaataca cccccgtatt gttcagaaga
     2041 tcggcgtagt aaattgagtc tgtcattttc ctcagttcat attctctctt gcaggaaagc
     2101 cagtattcca ctgcactgtt cagatttcct tttttcatat gaatgtcccc gagatagttc
     2161 agtatttcca gatacccgac agtatggatg agattcatgg actcgctcat tgtcttggct
     2221 ttcaggaaat actcaagtgc agtgctgaat tcccccctgt tgaagaaaac tacgcccaga
     2281 taatagtaaa cacgtatata attaaatgta ataacttctt cattctttct cagtatttcc
     2341 tgaaccctat tcaaaagcct ctcagagtca ttcagatact ctcctttccg gctcagcatc
     2401 acagcagaat ctatcaggag ttcagggatg ctcccatcgc ccgaaagtgc ctgtgaaaaa
     2461 agcagaatgg attcttcagg atttgttttt ctgagagaaa gtgctcttga ataagaaaaa
     2521 aaaccgttct ctgaaaactc aggatactga agaaatcgtt ccttttcctt ttcagtaaat
     2581 gatgcaggaa caaatccagg ccctgcagtc tcatttgctg cctgaatgat cttttcaaga
     2641 agaataagac ggagaagact gatttttccg actgaaccgg cacttacgaa gctttttttt
     2701 ctgaccggac tccttacgtt tgtcatctcc agatagacaa tgatgtcgga tccgttcagc
     2761 cgtactgaac ctttcagaat aagatccatt tcagtcttat cacggatcat cctttctctt
     2821 tcaggtctgg gaatctgtct tttcacgttt ccgtaggcaa atttcatatc ttcatattct
     2881 gaaagtcgga cagatgcaat ctgttcaatt ccatatgtca caagtctctg aatgcctgcc
     2941 gatacccacc caccctgctc ttccctttcc tcactgaata tttctgtgtg tatgacaatc
     3001 tttctttctt cagaagaaag acctgtaaac aggatgagca gaagcataaa aaaatatttc
     3061 ataatgattc cttaatgaat aaatgcataa acagaaccga gtatccgaca agaagcttct
     3121 aaaggaaaga tgcttctgcc gtatcagtcc gtccagaaac acattaaaat ctgtgcagaa
     3181 aggcttgcaa gaaaatatcc gggaaaagtc cctgacagtg ttttctgaat ttatgaaatc
     3241 aaaaattgag tatacaaata aaaaaggact gaattaaggt tcagaagaaa ggagtatgaa
     3301 tacaatgaaa gaattaaaga actttgtgat ggtttcctcg ctgctcttca ccttatctgt
     3361 ctgcagttct gccgataaaa aaaatgatgc accgcc
//
LOCUS       DAOQGJ010000183         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16163_length_3393_cov_2.831935, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000183 DAOQGJ010000000
VERSION     DAOQGJ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16163_length_3393_cov_2.831935"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PK683_04055"
     CDS             <1..762
                     /locus_tag="PK683_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07650.1"
                     /translation="KKHMLNLGSETVIEDPFPLFSGLENIQWVIHRMKEKNTFTVMNH
                     PELNGAFDASSAAKLSGYNAIEVISVFGTSVHILDSVLSAGRISFAMATDDLHYFSDS
                     IAETIKSPAYKNVFQKYTRFAKENGETMKRYIQVDTDELNESSLFENLCRGNYISVEK
                     PHAAMPDTEILQLDYNDGIITVRLRSRGNISFVGFRGKVLKEETDTTASEYEVQKSDP
                     YVRVSVVHNGNLVLSNPFFRYDGSYSRLSCTGEKK"
     gene            759..2414
                     /locus_tag="PK683_04060"
     CDS             759..2414
                     /locus_tag="PK683_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNH07651.1"
                     /translation="MIIYILIVLFTFLFYIPYLGTMPLFDWDELNFAESAREMLITGD
                     YFRVTVNFLPFWEKPPLFFWMQALSMKVFGVNEFGARFPNVLAGAATFSLIYHIGRKY
                     YDSLFGILWVLSFFGSFLPHLYFKTGIIDPFFNLLIFSGIFFIFKALNTDPLLAGNEV
                     KIQKWRTKNFLFSGLLIGTAILTKGPAALLITLLCAGAYYVLNGFRKYVKFKEILIFG
                     TAVFCISFFWFGPETIINGFWFIQRFVMYQLELAGTSQAGHGQPVYYHAVVLFFGCFP
                     VSMIFLFGRVSGVQEDGLKKNLNLWMAILFWTVFILFSLITTKILHYSSLCYLPMTYF
                     AARKIRYTILQRQEMKITRTAVLSLGAVFSAVFALLPYAASRMKDFAHLGKDEYIQES
                     MKIQVNWEMYGSAAGIILAAVIILYFILSRKNQFLKAYGVLFLGTAFVFEFLLFYLVP
                     KINEYTQAELINFYREAGTKNAYLEVVGYKSYGYLFYSKKQPSARKEAYDKNWLLFGE
                     TDKDSYFSVKTGTGDMYRLSPGIKELYRKNGYIFMMRPAQKKE"
     gene            2411..>3393
                     /locus_tag="PK683_04065"
     CDS             2411..>3393
                     /locus_tag="PK683_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07652.1"
                     /translation="MKRVILCADVYRPELHLDLSFQQEWDSPESIEFLKTAVQSAGIE
                     CEIIEPVKERGRLLRLLAEVAPEQKKNLILWNLTEGFLSRNREAYIPALAEFLGFPYT
                     GADAYAQILSLDKLLSKELAGKAGIPVTAHSEILPREFIVSVPEFPVFLKPRYEGSSL
                     GVSENSICRNWEELYINTGRISEDLFPLLAETYLEGREFTAGFIGERIGAPLEVISPS
                     PVYSEEVKGKSSMPEKLIPVEDASIDNTIMMYTRRIIETLGVRCYGRVDWKLNGNGSL
                     FFLEINLTPGLSPYYSSFPRSYENRLGSYSDMVKEILLLADANYELRQARYG"
BASE COUNT          940 a          675 c          775 g         1003 t
ORIGIN      
        1 aaaaaacata tgctgaatct gggttctgaa acggtcatag aagatccatt tcctcttttt
       61 tccggtctgg aaaacatcca atgggtgatt cacagaatga aggagaaaaa cacatttact
      121 gtgatgaatc atcctgaact gaacggggca tttgatgctt cttcagcagc gaaactttcc
      181 ggatacaatg ccattgaggt gatttctgtt ttcggcacct cggttcatat tctggattct
      241 gttctttctg ccggcaggat cagttttgca atggcaactg atgatctgca ttatttctcc
      301 gattcaatag cggaaaccat caaatcccca gcttacaaga atgtatttca gaaatacacc
      361 aggtttgcaa aggaaaacgg agaaacgatg aaaagataca ttcaggtgga tacggatgaa
      421 ctgaatgagt cttctctctt tgaaaatcta tgcagaggaa actatatttc tgttgagaaa
      481 cctcatgcgg ctatgcccga tactgaaata cttcagctgg attataatga cggaatcatt
      541 actgtaagac tcaggagcag aggaaacatc agctttgtag gttttcgcgg taaggttctg
      601 aaagaagaga cggatacgac ggcatcagaa tacgaagtgc agaaatctga tccatatgtc
      661 agagtatctg tcgtgcacaa tggaaatctt gttctcagca atccattttt ccgttacgac
      721 ggttcttaca gccggctgag ctgtacagga gaaaaaaaat gatcatatat atacttattg
      781 tattattcac tttccttttt tacattccct atctcggcac tatgcctctt tttgactggg
      841 atgaactgaa cttcgcagag tctgcaaggg agatgctgat tactggagac tattttcggg
      901 taactgtcaa ttttcttccg ttctgggaaa agccgcctct ttttttctgg atgcaggctc
      961 tttcaatgaa ggttttcggc gtgaatgaat tcggggcaag atttccaaat gttctcgccg
     1021 gagctgcgac attcagcctg atttatcata taggaaggaa atattatgat tctcttttcg
     1081 gaatcctctg ggtgctttca ttcttcggat catttcttcc tcatctgtat ttcaagacag
     1141 ggattattga ccccttcttc aatcttctga ttttttcggg aatctttttt atattcaagg
     1201 ctttgaatac tgatccgctt ctggccggaa atgaggtcaa gattcagaaa tggagaacaa
     1261 agaattttct gttttcaggt cttctgatcg gaacagcgat tctcactaaa ggaccggcgg
     1321 ctcttctgat cactcttctc tgtgcaggag catactatgt tctgaacgga ttcaggaaat
     1381 atgtgaagtt caaggaaata cttatattcg gaacagcagt gttctgcatc tcttttttct
     1441 ggttcggtcc tgaaacgatc atcaacggat tctggttcat ccagagattt gtaatgtacc
     1501 agctggaact ggcaggcaca tcacaggcag gacacggtca gcccgtctat tatcatgcgg
     1561 tagtgctttt tttcggatgt tttcctgttt caatgatctt tctcttcgga agggtaagcg
     1621 gagttcagga ggacggtctg aaaaaaaatc tgaacctctg gatggcgatt ctcttctgga
     1681 cagtgttcat tctgttttca ctcattacca ccaagatact tcattattct tcattatgtt
     1741 atctcccaat gacatatttt gctgcacgga aaataagata taccattctt cagaggcagg
     1801 aaatgaaaat aaccagaaca gcagttcttt ctctgggcgc agttttttca gcagtatttg
     1861 ctcttctgcc ttatgcagct tcccgtatga aggattttgc tcatctggga aaggatgagt
     1921 atattcagga aagcatgaag attcaggtga actgggaaat gtacgggagc gcagccggaa
     1981 tcattcttgc ggcagtcatt attctgtatt ttattctttc cagaaaaaac cagtttctca
     2041 aggcatatgg cgttcttttt ctagggactg cgtttgtttt tgaatttctt ctgttttatc
     2101 ttgttcctaa aatcaacgag tatacacagg cagaactgat caatttctac agagaagcag
     2161 gcactaagaa tgcctatctg gaagtagtcg ggtacaaaag ttacggttat ctgttttatt
     2221 caaaaaaaca gccttcggcc agaaaggaag cgtatgacaa aaactggctt cttttcggtg
     2281 agactgacaa ggacagttat ttttctgtaa agacagggac aggcgatatg tacaggcttt
     2341 ctcccgggat caaggagctg tacagaaaaa acggatatat tttcatgatg agaccggctc
     2401 agaaaaagga atgaaaagag taattctgtg tgctgatgtt tacagacccg aacttcatct
     2461 ggatttgtct tttcagcagg agtgggactc tccggaaagc attgaatttc tgaagacagc
     2521 cgttcagagt gcaggcattg aatgcgagat tattgaaccg gtaaaagaga gaggcaggct
     2581 tctcaggctt cttgcagaag tggctcctga acagaaaaaa aatctgattc tctggaatct
     2641 tacggaaggt ttcctgtcca gaaacaggga agcgtatatt cctgcactcg cagagtttct
     2701 gggatttccg tataccggtg cagacgccta cgcgcagata ctcagtctgg ataagctgct
     2761 ctccaaggag cttgcgggaa aggccgggat tccagtcact gcacattcgg agatcctgcc
     2821 gagagaattt atcgtaagtg ttcctgaatt tcccgttttc ctgaaaccca gatacgaggg
     2881 atcgagcctt ggtgtgtcag aaaattcaat ctgcagaaat tgggaagaac tttacataaa
     2941 caccggaaga atctctgaag atctgtttcc ccttctggca gaaacatatc tggaaggacg
     3001 tgagtttact gcaggattca tcggagaaag aataggagct ccccttgaag tcatttctcc
     3061 ttctcctgtc tacagtgagg aagtcaaagg aaaatcatca atgcccgaaa agctcattcc
     3121 tgttgaagat gcaagtattg ataataccat tatgatgtat accagaagaa taattgagac
     3181 tctgggtgtc aggtgttatg gaagagttga ctggaagctg aatggaaacg ggagcctgtt
     3241 ctttctggaa ataaatctga ctccgggact ctctccgtac tacagcagct ttcccagatc
     3301 ctatgagaat aggctcggaa gttattcgga tatggtgaag gagattctgc ttcttgctga
     3361 tgcaaattat gagctcaggc aggcaaggta cgg
//
LOCUS       DAOQGJ010000184         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16205_length_3387_cov_3.218187, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000184 DAOQGJ010000000
VERSION     DAOQGJ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16205_length_3387_cov_3.218187"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PK683_04070"
     CDS             <1..571
                     /locus_tag="PK683_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07653.1"
                     /translation="KLSLTVIDGAASTAENSDKGSEIAYELGEDAKFAQKSKTEFSNK
                     LVNEDFGFGEFWTGFREHFLKETPADLIKEKLVKFPVSVKGPMDDNPELKIKDKNFKD
                     LIAALKASPTGTNKENPKETEADFVKAKEKLHPKNDVVTEENFRLSNMVFSKSDEKWH
                     LVQVYLDEENYGKMKAFGGAPAPKKPNKK"
     gene            complement(618..1994)
                     /locus_tag="PK683_04075"
     CDS             complement(618..1994)
                     /locus_tag="PK683_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNH07654.1"
                     /translation="MSLIFRPSRDGSPNLFDSQLNLSVHSAFDPFKEASRLILSSFSK
                     DKIPAVYGFGAGHLVRVLLENFQFDSDQIFCIRSGLNEEMIPEIQPVLQDLLTKALQA
                     GKKLRFCDYSEIQKENYNVYILPYYIRKRSEEKASSALKVQSEGSGTGINEATKSFFS
                     RLWFRNYLININRYIEDSSSFFLTEKSFLLGRRPVLFIGAAPSLERQIDALKDKRDSL
                     FIICSDTASRFVRRKGILPDLVISLDSGRGTLYHIADSFPETVPILTWFGANREIFNL
                     KNTKVIYLSSYPLDQILSGLSEGKLECLKNPGLNVSGMAKSVSIYLKSSAYITAGTGF
                     TSESGKSHCRGTGYEMYNQDKIMRKTSAYSLQTRIYSDKISAKNMIAEKFLSSSELIR
                     TYTIEEFKFDSILKTGNSSEAEKFSGKDICRKLISVLQNGMYSQEIEKFSGLPSEIQR
                     RIQNRVLS"
     gene            complement(1998..2453)
                     /locus_tag="PK683_04080"
     CDS             complement(1998..2453)
                     /locus_tag="PK683_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNH07655.1"
                     /translation="MSSNNLNNEIDIQKVIQLARAKKYEVTSAGFAVLDKIDKINFSK
                     KMKGRKPAVLAIHALSDKMVKYDYFTPEQRKKLLQEAGLSDTPYKSFKGLFSNTSPAY
                     ASDESEEEFIAEETSSKPPIFMEDDDFEEATDDFEEDEEDDNFEEDEED"
     gene            complement(2505..>3387)
                     /locus_tag="PK683_04085"
     CDS             complement(2505..>3387)
                     /locus_tag="PK683_04085"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07656.1"
                     /translation="VVLGLVDDDTGLMCYINAEHPYSVLYRDGKAEFMDSQLLLRKIG
                     TTEMDGRLKVQTFRLQPFDVMVIGSDGRDDILLGIDENGQRIINEDEGKFLRHVEKGG
                     GELKGIFDAVKASGELTDDLTLLKVEYRPEEKSNEQEKIVDFQLLDSAEKLFKEDRWR
                     DYLHIAEKILEIDSIHKITLFRSGYASFFCKDFKSAVHFLESYVSFYPEDEEGFVFLG
                     AAYKHSGKYREAADIGEIIRLRNPKHLKNLLNLADSYRLMGNFERSMKMISEVRILHP
                     DNRTALEIETALQEHRK"
BASE COUNT          984 a          719 c          640 g         1044 t
ORIGIN      
        1 gaaactgagt ctgactgtta ttgacggggc tgcttctact gcagaaaatt cagacaaagg
       61 atccgaaatc gcgtatgaac ttggagaaga tgctaaattt gctcaaaagt caaaaacaga
      121 attttccaat aaacttgtaa atgaagattt tggatttgga gaattctgga caggattcag
      181 agaacatttt ctgaaagaaa ctcctgctga tttaattaag gaaaaactgg tcaagttccc
      241 tgtatcagta aagggtccta tggacgacaa tcctgaattg aaaatcaagg acaaaaactt
      301 taaggacctg atcgctgcgc tcaaagcgag tccaacagga acaaacaagg aaaatcctaa
      361 ggaaactgaa gcagatttcg taaaggcaaa ggaaaaactt caccctaaaa atgatgttgt
      421 gactgaagaa aatttccgtc tttccaatat ggttttcagc aagtcagatg agaaatggca
      481 tttagtgcag gtttatctgg atgaggaaaa ctacggtaaa atgaaagctt ttggcggagc
      541 acctgctcca aaaaaaccta acaaaaaata aaacaaaaca tagggactgt cacacagtcc
      601 ctttatttct ctatccttca agaaagaacc ctattctgaa ttcttctctg aatttctgac
      661 ggcagtcctg agaatttttc tatttcctga ctgtacatcc cattctgtaa aacagaaatc
      721 agttttctgc agatatcctt tcctgagaat ttttctgctt cagatgaatt ccctgttttt
      781 agaattgaat caaatttaaa ttcctctata gtataggttc ttattaattc tgaagaactg
      841 agaaattttt ccgcaatcat attctttgca gatattttat ccgaatatat tctggtttga
      901 agagaatatg cagatgtttt tctcattatt ttatcctgat tatacatttc gtagccggtt
      961 cccctgcagt gcgattttcc ggattcagaa gtaaagcctg tccctgcagt aatgtacgca
     1021 gaagatttta aataaatgga aactgatttg gccattcctg atacattcag accgggattc
     1081 ttcaggcatt ccagtttccc ctctgaaaga ccggagagga tctggtcaag gggatagctg
     1141 gaaagataaa taactttagt atttttcaga ttaaaaattt cccggtttgc accgaaccat
     1201 gtaagtattg gaactgtctc gggaaatgaa tcagcaatat gatacagtgt acctcttccg
     1261 gaatccaggc ttatgacaag atccggtaaa atcccttttc tccggacaaa tctggatgca
     1321 gtatcagaac atataataaa aagcgaatct cttttatcct taagtgcatc aatctgtctt
     1381 tccagagacg gagctgcgcc gataaaaagc actggacgcc tgcctaggag aaatgatttt
     1441 tccgtcagga aaaaagatga actgtcttca atatatctgt ttatgttgat cagataatta
     1501 cgaaaccaga gtctcgaaaa aaaagatttg gtagcttcat tgatcccggt gccgctgcct
     1561 tctgactgca ctttcagtgc agaggatgct ttttcttcag atctttttct gatataatat
     1621 ggaagaatat atacgttata attttctttc tgaatttcag aataatcgca gaaacgcagt
     1681 ttctttccgg cttgaagtgc tttcgtaaga agatcctgaa gtaccggctg aatttcagga
     1741 atcatttctt cattcagacc gctcctgatg cagaaaatct gatcggagtc aaattggaaa
     1801 ttttcgagta agactcgaac cagatgccct gcaccgaaac cgtaaacagc aggaattttg
     1861 tcttttgaga aactggacag aattaatctg gaagcttcct tgaaaggatc aaaagctgaa
     1921 tgtacggaga gatttagctg tgaatcaaag agatttggac ttccgtccct tgagggacga
     1981 aagattaaag acatacttca gtcttcttca tcctcttcaa aattatcgtc ctcttcatct
     2041 tcttcaaagt catccgttgc ttcttcaaag tcatcatctt ccataaaaat aggaggtttg
     2101 cttgaggtct cttccgcaat gaattcttct tcactttcat cagatgcgta tgcaggtgaa
     2161 gtattggaaa aaagtccttt gaagcttttg taaggagtat ctgaaagccc ggcttcctgc
     2221 agaagtttct ttctctgttc tggtgtaaaa taatcatact taaccatttt gtcggaaagt
     2281 gcatgaatcg ccagaacagc aggttttctg cctttcattt ttttcgaaaa gttgatcttg
     2341 tcaatcttgt ccagcacagc aaatcctgct gaagtcactt catatttttt agcgcgtgca
     2401 agctggatca ctttctggat gtcgatttca ttgttcagat tatttgaaga cataggctct
     2461 tatgacattt ttttttctgc cagctttttg tcaatacgtt aaccttattt tcgatgctcc
     2521 tgaagtgccg tctctatttc cagtgctgtc ctgttgtcag ggtgcagaat tctaacctcg
     2581 gaaatcattt tcatggaacg ttcaaaattc cccatcagtc tgtaagaatc agccagattc
     2641 agcagatttt tcagatgttt aggattcctg agtctgataa tttccccgat atctgccgct
     2701 tccctgtatt ttccggaatg tttatatgcg gctcccagaa atacaaatcc ttcctcatcc
     2761 tcaggataaa aagatacata ggattccaga aaatgaacag cactcttgaa gtccttgcag
     2821 aaaaaacttg catagccgga tcgaaaaagc gtaattttat gaattgaatc aatctccaga
     2881 attttttcag cgatatgcag atagtcccgc catctgtcct ccttgaacag tttttctgca
     2941 ctgtcgagaa gctgaaagtc cacgatcttt tcctgctcat tcgatttttc ttccggcctg
     3001 tactctactt tcagaagggt aaggtcgtca gtcagttctc cggaggcctt cactgcatcg
     3061 aaaattcctt tcagttctcc cccgcccttt tctacatgac ggagaaactt tccctcatct
     3121 tcattgatga tcctctgtcc attctcatct attccgagca gaatatcatc ccgtccgtct
     3181 gaaccgatga ccattacatc aaacggctgg agccggaatg tctgcacttt caggcgcccg
     3241 tccatttctg tagtccctat tttccgaaga agaagctgtg agtccataaa ttccgccttc
     3301 ccgtctctgt acaatacact gtacggatgc tctgcattga tataacacat caggccggta
     3361 tcatcatcca ctagccccag aacaact
//
LOCUS       DAOQGJ010000185         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16224_length_3384_cov_3.812857, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000185 DAOQGJ010000000
VERSION     DAOQGJ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16224_length_3384_cov_3.812857"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..2485
                     /locus_tag="PK683_04090"
     CDS             248..2485
                     /locus_tag="PK683_04090"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNH07657.1"
                     /translation="MFEKNAVLDRQEKTEGAENRPPVISINGQTIIGSRNEDILSAAL
                     QEGLDFPHSCRVGGCAACKCRLIEGKVKELTETGYLLTEEELNSGYILACQSIPETDL
                     KIELENEPPQKYFGKIIGQKKLNHDICEISIQLDRSIRYKAGQYASLSLEGLPGIERN
                     YSFASPVEDGNTVNFFIKKMPDGKFSSYVHENSLIGKSVQLKGPYGEFWLRPGKNHIL
                     MMAGGSGLAPILAILRQALKDRTYRPVTLLFGAKKQEDLYRLEEIRNIQKQWPTEFNF
                     IPVLSEEDENSLWNGARGFASDIIYNFIESGSEAYLCGPPPMVNSARDLLIRNGVSRK
                     SIYADPFTVGTGRTDESKGEEKKGTAGFFHYFKYSLFHIIGLLAAFTILAGGNYTVFG
                     LIGLLMLYILGDAVSGNDITVPEYKYPKILTFQLWMALPLLMFIIFSSVWSVSSTDSF
                     GFGAWAGKLTGYDFLQAREISGWIHHFCGFITAGLMIGLVGTITAHELTHRTWDPASM
                     FIGRWLLAFSFDTIFSIEHVYGHHRYVSTVEDPATAPRGRNVYYHIISSSIKGNISAW
                     KIEASRLRKKHLPVISVHNAFLRGHLMSVFLVFMAWMTGGMEGMLFFTACAVWGKALL
                     EIVNYMEHYGMVRLPEEPVQPRHSWNTNRRVSSWTMFNLTRHSHHHAQGEVPYHKLRP
                     YPEAPMMINGYLTTIVIALIPPLWTRLMIPKVIEWDTKYASPEEQELIKAANRKSGIP
                     ELMKR"
     gene            2781..3290
                     /locus_tag="PK683_04095"
     CDS             2781..3290
                     /locus_tag="PK683_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH07658.1"
                     /translation="MKILFMIMTISLCPLYAQAQKKKIPNRLIDYPKFQKTVNDSAEE
                     RESRRLTEEEFLKMMKEEGAVLLDARTESRYSMRHIKGAVSLPFTEFTESSLSSVIPS
                     KTSKILIYCNNNFTGDQQSFASKMPAASLNISTYNALKVYGYKNIYELGPLLDISRTK
                     IPFEGSLVK"
BASE COUNT         1019 a          716 c          766 g          883 t
ORIGIN      
        1 cgggcgcact gaaaaagtaa gtcccagcga ttcgagagcc tgctttcggc gaggcgaggc
       61 ggattttaga atgagcataa ttcctttttc tggtggcata ttcctctgtc cagtcccgtt
      121 ttgattttta gaagggggct tggatcgttt tattttttat aacaaatgtt ttaaaaaaag
      181 attgaccgaa aattcacatg gcagtatatg tatatcgaac gtttgttata aaaagaggct
      241 aaagactatg tttgaaaaga atgcagtatt ggataggcag gaaaaaaccg aaggggcaga
      301 aaaccgccct cctgtgatca gtatcaacgg gcagaccatc atcgggtcaa ggaatgagga
      361 tattctttct gcagcactgc aggaggggct ggattttccg cacagttgca gggtcggcgg
      421 atgtgcagcc tgcaaatgca gactcattga aggaaaagta aaagaactta ccgaaaccgg
      481 ctacctgctg actgaagagg agctgaactc cggctatatc cttgcctgcc agagcattcc
      541 cgaaaccgac ctgaaaatag aactggaaaa tgaaccccct caaaagtact tcggaaaaat
      601 aatcggacag aaaaaactga atcatgatat ttgtgaaatt tcgattcagc tggacagatc
      661 catccggtac aaagccggac agtatgcttc tttgagcctg gaaggactgc cgggaattga
      721 aaggaactat tcgttcgcat ctccggttga ggacggcaat accgtaaatt tttttataaa
      781 aaaaatgccg gatgggaaat tttccagcta tgttcatgaa aacagtctga tcggcaaatc
      841 agtacagctg aaaggtcctt acggtgaatt ttggctcaga ccgggaaaaa atcatatact
      901 gatgatggca ggcgggagcg gtcttgcacc tattctggct attttaagac aggcactgaa
      961 agacagaaca tatcgtccgg tcacacttct gttcggagcc aaaaaacagg aggatcttta
     1021 cagactggaa gaaatacgga atatccaaaa acaatggccg actgagttta actttattcc
     1081 tgtgctctcg gaagaagatg aaaacagtct ctggaacgga gcaagagggt ttgcctcaga
     1141 tataatatat aattttatag aatccggttc agaggcttat ctttgcggtc ctcctccgat
     1201 ggtcaattca gcccgagatc tgcttataag gaacggggtc agccggaaaa gcatatatgc
     1261 tgatccattt actgttggaa ccggcagaac agatgagtcg aaaggcgaag aaaagaaagg
     1321 gactgccggc ttctttcatt atttcaaata ctctctgttt catattatag gactgcttgc
     1381 tgcatttaca attcttgcag gaggcaatta cactgttttc gggctgatcg gtctgctgat
     1441 gctgtacatt ctcggggatg ctgtatcagg aaacgacata acagttccgg aatataagta
     1501 tccgaaaatt cttacttttc agctatggat ggccctgcct ctcctgatgt tcatcatctt
     1561 ttcttcagtc tggagcgtaa gcagtactga cagtttcgga ttcggagcct gggccggaaa
     1621 actgactggt tatgattttc ttcaggcaag agaaatatcc ggatggatcc atcatttctg
     1681 cgggttcatc accgcaggtc tgatgatagg acttgtaggc acaatcactg cccacgaact
     1741 tactcacaga acctgggatc cggcttcaat gttcataggc cgatggcttc tggcattcag
     1801 ttttgacaca atattttcca ttgagcatgt ttacggacat cacagatatg tctctacagt
     1861 cgaagatcca gcaacagccc caagaggaag aaatgtatac tatcatatta tatcctcttc
     1921 tataaaagga aatatcagtg catggaagat agaggcatca aggctgagaa aaaagcatct
     1981 cccggtcatt tcagttcaca atgcatttct tcgcggacac ctgatgagtg ttttccttgt
     2041 ctttatggcc tggatgaccg gaggaatgga aggtatgctt ttcttcacgg cctgtgcagt
     2101 atggggcaag gcacttcttg aaatcgtaaa ttatatggaa cattacggaa tggtcagact
     2161 gcctgaagaa cctgttcagc ctagacactc ctggaacaca aaccgaagag tgagctcctg
     2221 gacaatgttc aatctcacca gacattctca tcatcacgcg cagggcgagg ttccctacca
     2281 taagctaagg ccctatcccg aggcaccgat gatgatcaac ggatatctga ctacgattgt
     2341 aattgcactc attcctcctc tctggaccag actgatgatt ccgaaagtaa ttgaatggga
     2401 tacaaaatat gcaagtccag aagaacagga actcattaaa gctgcaaacc ggaaaagcgg
     2461 aataccggaa ttgatgaaaa gataaattgg gctgagaatg ctttttcatg cggaggcctt
     2521 aggtgcactt cacggactta gcaagcttgg aagccggtgc tcagaacaag cctgtcgaac
     2581 tgtgagcctc ataaagcatt ctttgacgag gctcagaatg acaaataatc actaattgta
     2641 cccgctcccg ttatttattt ccaatgtccc aaaggcggag agcagcagga atgtgctttc
     2701 cgcaagtctg ccatgcttcg gtgtcagaaa tccggggatt tattattttc acagttcagg
     2761 acttgatcag tctaattttt atgaaaatac tgtttatgat aatgaccata tccctgtgtc
     2821 ccctctatgc gcaggcacag aagaaaaaaa taccgaacag actgattgac tatccgaaat
     2881 ttcagaagac agtcaatgat tcagcagagg aaagagaatc cagaaggctg actgaagaag
     2941 agtttctgaa aatgatgaaa gaagaaggtg cagttcttct agatgcaaga acagaatcca
     3001 gatacagtat gaggcatata aaaggagccg taagtcttcc ttttacagaa ttcactgaat
     3061 cctcactttc ttcagtaatc ccgtccaaga cttcaaaaat cctcatctac tgcaacaata
     3121 actttacggg ggatcagcag tcctttgcct ctaaaatgcc ggcagcttca cttaatattt
     3181 ccacttataa tgctctcaaa gtctacggtt ataaaaacat ttatgaattg gggcctctgc
     3241 tcgacatcag caggactaaa attcccttcg aaggaagtct tgtgaaatag ctgcctgaaa
     3301 ctgagactgc gaaaaaatct aatttataaa atattctaca ataaactcat ctatttctcg
     3361 gcagactgac aggagagtct gcct
//
LOCUS       DAOQGJ010000186         3372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16311_length_3372_cov_2.593910, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000186 DAOQGJ010000000
VERSION     DAOQGJ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16311_length_3372_cov_2.593910"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PK683_04100"
     CDS             complement(<1..712)
                     /locus_tag="PK683_04100"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNH07659.1"
                     /translation="MAKDKEDSLFKEIVAVCKRRGFVFPGSEIYGGLSNSFDYGPYGV
                     EILNNLRRLWWEYFVHRRTDVVGLDSSILLNPAVWEASGHVGNFSDPLIDCKSCKVRF
                     RVDHFLEEKKGEGFSNGKTLAELQEVLNQEDYACPKCGTKKSFTEARNFNLMFKTQQG
                     ANEGGTVDIYLRPETAQGIFINFKNVYTSCRKRIPFGIAQIGKSFRNEIVARQFVFRT
                     REFEQMEMEYFCAPGTQKE"
     gene            complement(838..1758)
                     /locus_tag="PK683_04105"
     CDS             complement(838..1758)
                     /locus_tag="PK683_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH07660.1"
                     /translation="MKEIHLEGHTITYEEFGTGNEEFVFLHGWCSCRVIWDTVIDDFT
                     VLGKCYTIDLPGHYPGIAPAGFRKFTFRDVVDLQAAAIRKMVKNEKVTLVGHSVGGLV
                     AIALSVYHPDLVDRVVAICPALSGPVTGFFYFLKLAMDMHWGFLLEGAWKVFHLPETM
                     AQFFSLGTYDHDRFMKLPETREFLNDYGENLAKLEPSVMASYLQMFDVTDFRPQLPNV
                     NVPALIIATKHDKIVPEKYHHYAAAHIPGAELIQLNNSGHVPTIEERDLCIPGIIEWL
                     KKTDSETKSEKEISNKKTVVRKKASAQHNR"
     gene            1986..2648
                     /locus_tag="PK683_04110"
     CDS             1986..2648
                     /locus_tag="PK683_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07661.1"
                     /translation="MLVVLLLANCLGVSRSKAVIAAAAQLKAQSVLDANPVSRPPALV
                     NPDGTPLHPAVDEKNIYEISVKDGEEIAEFEETAATQKLLEKENMAKAMSDFQKFFYY
                     PNLTSAQVIEDAEDADLGLPPKDRSLTDSEKQKRVQEETERVQKFHAEIRSFIYDATP
                     DELKNMRTNLTLAIAVHGRLGEYIIGLSSAEQLYFKKIIEYNIQSRMLVIDAMTAGGI
                     KQ"
     gene            2645..3199
                     /locus_tag="PK683_04115"
     CDS             2645..3199
                     /locus_tag="PK683_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07662.1"
                     /translation="MRSMILLIVLFLIECSNQPSKNRQKEEQFGIRQPMFWWMLLANR
                     PKDEDPNCGGDPLETLFGLNKDKLNQFTPLAENQEIVTDFIDNSYKYTNDISYLSSKK
                     SEIQITPIEIQSNTECCSTSFKASSAAAYCSGVKSIVFFPSQKYLLLETIKISAVTGS
                     KNLVSTGHSPGECFVKFKIKSVVL"
     gene            3165..>3372
                     /locus_tag="PK683_04120"
     CDS             3165..>3372
                     /locus_tag="PK683_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07663.1"
                     /translation="MSNLKLKAWYCNENTGKKIICAVLLLLYMSELFAIPPFKTHLDT
                     ENIDDQKRCQTASLYDDNTAITFFG"
BASE COUNT          947 a          754 c          690 g          981 t
ORIGIN      
        1 attctttctg agttcctgga gcgcagaaat attccatttc catctgttca aactctcttg
       61 ttctgaatac gaactgcctt gcaacgatct catttctgaa tgatttcccg atctgcgcga
      121 ttccgaacgg aattcttttt ctgcaggatg tatatacgtt tttgaaattg atgaaaatcc
      181 cctgagcagt ctccggtctg agatagatgt ctacggttcc gccctcattt gctccctgct
      241 gagtcttgaa cataagattg aaatttcttg cctcggtgaa gcttttcttt gttccgcact
      301 taggacaggc atagtcttcc tgattcagaa cttcctgaag ctcagccagc gtcttaccgt
      361 tcgaaaaccc ctctcccttt ttctcttcca ggaaatgatc cacgcgaaat cttaccttgc
      421 aggacttgca gtctatcaga ggatctgaga aatttcccac atgtcctgat gcttcccaaa
      481 ccgccgggtt cagcagaatg gaggaatcga gtcccactac atcagttctt ctgtgaacaa
      541 aatactccca ccagagtctt cggagattgt tcagtatctc tacaccgtag ggaccgtagt
      601 caaaggaatt ggaaagtcct ccgtaaattt ctgaaccggg aaaaacaaat ccccttcttt
      661 tgcataccgc aactatttcc ttgaacaatg aatcttcttt atctttagcc atgtttgacc
      721 ttttctgtct ttttttcgtg actgctcagc cttaacttcg cagctgccgg tttatattgg
      781 aatatcaaca gtcttcaagg gattaaaaat cccgtaaaat ccctgctttt acatcttcta
      841 acgattatgc tgagcagatg cctttttcct gaccacagtt tttttatttg agatctcttt
      901 ttcagacttc gtttctgaat ctgttttctt cagccattca ataatgccgg gaatgcagag
      961 atctctctct tctatcgtcg gaacatggcc ggaattattg agctgtatga gttcggctcc
     1021 cggaatatgt gcagcggcat aatgatggta tttttccggt acgatcttat catgtttggt
     1081 tgcgataatc agtgcaggta catttacatt cgggagctgc ggtctgaaat cagtcacatc
     1141 aaacatctgc aggtaggatg ccattacgga aggctccagt ttggccaggt tttcaccgta
     1201 atcattcaga aattctcgtg tttccggaag cttcataaat ctgtcatggt cgtatgttcc
     1261 cagactgaaa aactgagcca ttgtctcagg aagatggaaa accttccagg ccccttccag
     1321 aagaaaaccc cagtgcatat ccattgccag tttcagaaaa taaaaaaatc cggtcacagg
     1381 accggagagt gcgggacaga tggcaacgac cctgtctaca agatcaggat gatatacgga
     1441 aagagcaatg gctacaaggc ctccgacaga atggccgacc agagtgacct tttcgttttt
     1501 caccattttt cgaattgcag cagcctgcag gtctacaaca tccctgaaag tgaattttcg
     1561 aaaacctgca ggtgctatgc cggggtaatg tccgggcaga tcaattgtat aacatttccc
     1621 gagtacagta aaatcatcga ttacagtatc ccagatcact ctgcaggaac accatccgtg
     1681 aagaaatacg aattcttcgt ttccagtacc gaactcttca taagtgattg tatgaccttc
     1741 caaatgtatc tctttcataa ggttagtttt tttttgcacc gaccggaatc aagtcctgat
     1801 ttttccgatt tgtcaaattg agaaggactg aaaatgcagg attgcccgac ttatccagaa
     1861 cttcttgaca tcctttcggc aatgtactaa acctgacact atgacccgtt ttcctgcccc
     1921 ccccacgtct tcgatccgtt gtaaagacgg attgtaatcc gtctctacga ccgcctctac
     1981 ttgctgtgct tgtggttctg ttacttgcga actgtctggg agtcagccga tccaaagcag
     2041 tcattgccgc agcagctcaa ctgaaagctc agtccgttct ggatgccaat cctgtcagca
     2101 ggcctcctgc tcttgtaaat cctgatggaa ctcctctgca tccggcagtg gacgaaaaga
     2161 acatttatga aatctccgta aaagacggag aagagattgc cgagtttgaa gaaacagcgg
     2221 caactcaaaa acttctggaa aaagagaata tggcaaaggc aatgtctgat tttcagaagt
     2281 tcttctatta tccgaatctg acttctgctc aggtgattga ggatgcagaa gatgcagacc
     2341 tgggacttcc tccaaaagac aggtcactga cggattcaga aaaacagaaa agagttcagg
     2401 aagagacaga gagagttcag aaatttcatg cggaaatacg aagttttatt tatgatgcga
     2461 ctcccgatga actgaagaat atgcggacaa atctcacgct tgcaatagcc gtgcacgggc
     2521 gtctggggga atacatcatt ggactcagtt ctgctgaaca attatatttc aaaaaaatta
     2581 tagaatataa tattcagtcc agaatgcttg tgattgatgc aatgacagcc ggagggataa
     2641 aacaatgaga tcaatgatcc tgcttattgt attatttctt atcgaatgct ccaatcagcc
     2701 gagcaaaaac aggcagaagg aggaacagtt cggaatccgt cagcctatgt tctggtggat
     2761 gcttctcgca aacagaccga aagacgaaga cccgaattgc ggaggcgatc ctctggaaac
     2821 tttgtttgga ctaaataagg ataaactgaa tcagtttacc cctcttgcag aaaatcagga
     2881 aattgttact gattttatag ataattccta taaatatacg aatgatattt cttatctcag
     2941 ttccaaaaag tcggaaattc aaattactcc gattgaaatt cagtcaaata cagaatgctg
     3001 cagtacttct tttaaggcaa gttctgctgc cgcatactgt tcgggagtaa aatccattgt
     3061 tttttttcct tcacagaaat atttgctctt ggagacaata aaaatttctg ctgtgactgg
     3121 aagcaaaaat cttgtttcca ctggtcatag tcctggagaa tgttttgtca aatttaaaat
     3181 taaaagcgtg gtactgtaat gaaaatactg ggaaaaaaat tatctgtgct gttctgcttc
     3241 tgctctatat gtcagaactg tttgcaattc cgccatttaa gactcatttg gatacagaaa
     3301 atattgatga tcaaaaaagg tgtcaaacag cgagcctcta tgacgacaac acggccatca
     3361 ctttttttgg ag
//
LOCUS       DAOQGJ010000187         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16440_length_3355_cov_2.154545, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000187 DAOQGJ010000000
VERSION     DAOQGJ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16440_length_3355_cov_2.154545"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2587
                     /locus_tag="PK683_04125"
     CDS             <1..2587
                     /locus_tag="PK683_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH07664.1"
                     /translation="YLQMYDADTGLMIDAHQSTAYLEAGTDLKLDEKELKNSDLDTIR
                     KDTEKAALKMKINISKKENRQSIEENLLNTSLGRDPSLKFPVAAENVAKQAEDVFDIL
                     KSTEVVTASRTKESLLNVPASTMVVTEQDFKNRGYTSLDDIFRDLPGFDYIGTMGTDN
                     ANLYQRGYRTPFTSRTLLMINGIIENDLWAQVATPSRQYPIANIKRVEVIYGPASAVY
                     GPNAFQGIINIITKDGKENEGRKVSGKTSFMYGSGNTWAVDGGITSQIGDFSLAISAR
                     KQEGDDANKNVSNYGYNSRYWVGHPTVWGPMLAKGDAGRPFGKYADPVNDWGTVISGS
                     YKTVKVGVNITNKYEGYGPTYPGDKSQPATMWGKSGISIYAENQVDLSEKVSSYTLLN
                     YRDSRIYGNWAEAVDDWNPGKEMYSYVSYTRWNEISKSLLGNQNIEYKFSSSLKLIGG
                     LKAEFKKLTKSYDVPGYWWGSSFSSVDYLNPDVNKGIDKVTPNGYSIMHSTDPYLLKG
                     PSPKNRMPDENTIGTYDRGAFLLSILEIGKFRFSPGIRYDHNSIYGQSINPRITGIYK
                     FDDRNAFKLLYGEAFNEPSPLNLFGGFSGRTADLKLKPEKVRTGEAIYMHQGKQALNE
                     FSVYYSRYDNVIKENAQNAGKRRIYGFEYKFRWNFSNFISGSAPISFYTYYTFTQAQS
                     SIYYDFQLGEWKEGDTFLGQYEYLFKDQAKNIPRRTKYSNLGDIAPSKINLGFNIPVK
                     NLFIINLRGNYVSRREFYLRNAQRNDYPIENDEESILRRITKEKERTLNPYFIADAGL
                     TFNFGDYGYLTIKIGNLFNKSYNHPGVGQGNSGNYYYERSLLNHPAAWKNVYAEYDSD
                     FLIKA"
     gene            3176..>3355
                     /locus_tag="PK683_04130"
     CDS             3176..>3355
                     /locus_tag="PK683_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07665.1"
                     /translation="MKKKDKDGLQTGSEFPDLGSVKWIKGKKAVLGKKLTAFFIWESG
                     TEDNIEAAAIFNDISE"
BASE COUNT         1120 a          625 c          770 g          840 t
ORIGIN      
        1 gtacctgcag atgtacgatg cagatacagg attgatgata gatgctcacc agagtacagc
       61 atatctggaa gcgggcacag atttgaaact ggatgaaaag gaactgaaaa actccgattt
      121 ggatacaatc agaaaagaca ctgaaaaagc agcgctgaag atgaaaataa acatctcgaa
      181 gaaggaaaac aggcagagta ttgaagagaa tcttttgaat acttcacttg gcagagatcc
      241 ttccctaaaa tttcctgtag ctgcagaaaa tgttgccaag caggcggaag atgtttttga
      301 catattaaag tccactgagg ttgtgactgc atcgagaaca aaggaaagtc tgctcaatgt
      361 tccggcctcc actatggttg tcacggagca ggactttaag aacaggggat ataccagcct
      421 ggatgatatt ttcagagatc ttccaggatt tgattatatc gggacgatgg gaacagataa
      481 tgcaaattta taccagagag gatacagaac tccttttact tccagaacac ttctgatgat
      541 caatggaatc attgaaaatg atctctgggc acaggttgcg accccgagca ggcagtatcc
      601 tattgcgaat ataaagaggg tggaggtcat ttacggaccg gcttctgctg tttatggacc
      661 aaatgctttt cagggtatca taaatatcat tacaaaagac gggaaagaaa acgaaggcag
      721 gaaggtatca ggaaaaacaa gtttcatgta cggaagcggg aatacatggg ctgttgacgg
      781 aggcattact tcacagatag gagatttcag tcttgcaatt tctgcaagga agcaggaagg
      841 agatgatgca aataagaacg tatccaatta cggatacaat tcgagatact gggtaggcca
      901 tccgaccgtc tggggtccga tgcttgcaaa gggagatgca ggcagaccct tcggtaaata
      961 tgcagacccg gtcaatgact ggggaactgt catcagcggt tcctataaaa cggtgaaagt
     1021 aggggtaaat attacaaata agtatgaagg atatggaccg acgtatccgg gtgacaagtc
     1081 acagcctgca accatgtggg gtaagtcggg gatcagcata tatgcagaaa atcaggtaga
     1141 tctttccgaa aaagtaagct cctatactct attgaattac agagacagca gaatttacgg
     1201 aaactgggca gaagcagtgg atgactggaa tccaggaaaa gaaatgtatt cctatgtcag
     1261 ttataccaga tggaatgaaa tcagcaaaag tcttctcgga aatcagaaca ttgaatacaa
     1321 attcagcagc agtctgaagc tgatcggcgg acttaaggcc gagtttaaaa agctgacaaa
     1381 atcatacgat gttcccggat actggtgggg atcttcgttc agttcagtgg actatctgaa
     1441 tcctgatgta aacaagggta ttgataaagt gactccaaac ggatattcta taatgcacag
     1501 cactgatcca tatctgctca aaggaccatc accaaagaac aggatgcctg atgaaaatac
     1561 tataggaact tatgatcggg gagcttttct tctcagtatt cttgaaatag gcaaattcag
     1621 attcagtcct ggaatacggt acgatcataa ctccatttat ggtcagtcga tcaatcccag
     1681 aatcactgga atttacaaat ttgacgacag gaatgcattc aagcttctgt acggagaagc
     1741 attcaatgaa ccttctcctt tgaacctgtt cggaggattc agcggaagga ctgcggatct
     1801 caagcttaaa ccggaaaaag tgaggaccgg tgaagcaatc tatatgcatc agggaaaaca
     1861 ggcactgaat gagttttccg tctattattc cagatatgat aacgtaatta aggagaatgc
     1921 tcagaacgcc ggaaaaagaa gaatttacgg atttgaatac aaattcagat ggaatttttc
     1981 caattttatc agcggatcgg ctcctatcag cttttacacc tattatacat ttacccaggc
     2041 acagagcagc atctattatg attttcagtt aggcgaatgg aaggaaggtg atactttttt
     2101 aggacaatac gaataccttt tcaaggatca ggcaaaaaat attccacgca gaacaaagta
     2161 ttccaatttg ggggacattg ctccttccaa gatcaatctg ggtttcaaca ttccggtgaa
     2221 gaatctcttc attattaatt tgagaggaaa ctatgtcagc agaagagaat tttatcttcg
     2281 caatgcccag agaaatgatt atcctattga gaatgatgaa gagagcatac tgagaaggat
     2341 tacaaaggaa aaagaaagaa cactgaatcc atatttcatc gcagatgcag gactcacatt
     2401 taatttcggc gattacggtt atctcacgat caagatagga aacctgttca ataaatccta
     2461 taatcatccc ggagtcgggc agggcaattc cgggaattac tattacgaaa gatctctcct
     2521 caatcatccc gcagcctgga agaacgtata tgctgaatat gactctgact ttctaatcaa
     2581 agcctgacac ctgcctgccg taagcttgtc ttttcaaagc actgaacaac gattcaggga
     2641 atggaaggtg cagcaattgt cataaaaaaa tcaagtcact cagcattttc agcaaaaaga
     2701 aaaaggaggg tttccttttt cattctttca atactgaatc cccgcccaaa aaaagagacg
     2761 atgcacgagg acagcccgaa attcatcttt ctgcagatca aaactgatta agattttctc
     2821 aaatgattga ctgaagaagt gaatcggatt tgattgccga atgagaaatc tgttcctgct
     2881 tttttatccg ttgttccacg caggaagaga ccgagatcca gttttaattt taggaaaggt
     2941 gagtttttga aataactgaa agcagattac ccggattttc cgcttattcc gaatttggaa
     3001 ggtttactgt gttcaaatgt aaacaaggca agggtgctga aggaatgagc ataatcagga
     3061 taaatgaaca gtccggtcag tttttacggc ctggctgaac agtcagatga gattcatcgt
     3121 ctgctccaga gaaaaagtca gccgggctga acatgattat gggagatata ttataatgaa
     3181 aaaaaaagat aaagacggac tgcagacagg aagcgaattt cctgatctcg gcagtgtaaa
     3241 gtggatcaaa ggaaaaaaag cagtccttgg aaaaaagctg actgcctttt tcatttggga
     3301 atccggcaca gaggacaata tcgaagcagc tgcgatattc aatgatatca gtgaa
//
LOCUS       DAOQGJ010000188         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16571_length_3338_cov_3.897350, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000188 DAOQGJ010000000
VERSION     DAOQGJ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16571_length_3338_cov_3.897350"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PK683_04135"
     CDS             <1..1165
                     /locus_tag="PK683_04135"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07666.1"
                     /translation="ASDERVGLDVLVRQKKKFEFKKKIHEIGGDICISDIITLQGRKY
                     TVFINGDAMGKSIQGAGGAIVLGVVFKSILSRTIMYQGNQAVYPERWLKLAFIELQKV
                     FESFDGSMLVSIVIGLIDNECGYLYFINAEHPWSVLYRDAKASFLESGFNLRKVGMLG
                     MEGSLFIRTFQMNRNDVIFIGSDGRDDIQLGEDETGRRIINENENLFLQTIEDAEGHL
                     EKILVLTEKKGEITDDFSLLRIRYMGTDSIMLNEKPEFMQESIALLQKDRYEEVLLTL
                     DLHKNDEMGEYATEFTELVGKTVSAVKNYRAGVNILNEFHLKHPEDEEAIYLLATYFY
                     LLKDFHKAADLGEILKLRSPEDPKILRLLVEIYTASGNIIRAQKILSGVQSTQ"
     gene            complement(1203..2063)
                     /locus_tag="PK683_04140"
     CDS             complement(1203..2063)
                     /locus_tag="PK683_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07667.1"
                     /translation="MNYYLVIGGSAVSGEQTISAIRSEDKDAYIISTSSKEGDVQNAD
                     ETFRNIDLRSEESISRISEGLKDRHLKSLIYIPARGMVGKPVQYADKAEYLDSLNFSV
                     IPMLKLTKVLKPGLSVWFSGFMWLKPLMLFYGTMTYTKITMEQITLQFPKQFKCIRYG
                     MFHSDSVRGISVLVQKSLNSNLYPEMNEYKEAWKKSGKKFKEFFPGMNYEFEESQLKT
                     ESSFDKPFRPLEPKDIQIGIKKALFYNTKPILNVVGDWYWENDSLPVWPAEIQKHMDI
                     ISADLDKYLK"
     gene            complement(2187..2978)
                     /locus_tag="PK683_04145"
     CDS             complement(2187..2978)
                     /locus_tag="PK683_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07668.1"
                     /translation="MSTKVYYTHENGGRPFKVEILSKNEVLIYKLDLSVDNNPSFESE
                     AELVFEKKPLFKYHPEKIFIGKSPETPSTLALGTAGAEWDGNTNLLQMENGYYVYVGG
                     CIFSFQPIAEITEFVSPMGNNDVPYPWAKDKKGNYYLPIEDTVLLQVPKGTEDPYTWF
                     YENAQRIYSSSKEVVLPRPEFEGIKHWYLGKEKWNIFWSHNPEWSFDALTEDGKVKMS
                     VETTDGKKKKITKEDYVSLNDKFGKELGLESIKKHKFKMLQKSLW"
BASE COUNT          958 a          664 c          697 g         1019 t
ORIGIN      
        1 cgcaagcgat gaaagagtgg gtctagatgt gcttgtaaga cagaagaaga aatttgaatt
       61 caagaagaag attcacgaga tcggcggcga tatctgtata tctgatataa ttacactgca
      121 gggaagaaaa tatacggtgt tcatcaacgg cgatgcaatg ggaaaatcca tacagggggc
      181 aggcggtgcg attgtcctcg gggttgtatt taaatctatc ctttcccgaa cgataatgta
      241 tcagggaaat caagctgtct atccggagag atggctcaag cttgcattca ttgagcttca
      301 gaaagtgttt gagtcctttg acggttcaat gcttgtttca attgtaattg gacttattga
      361 caatgaatgc ggatatctct atttcattaa cgcagaacat ccttggagcg tactgtatcg
      421 tgatgccaaa gcttcatttc tggaatcagg ttttaatctc aggaaagtgg gaatgcttgg
      481 aatggaaggt tcattgttta tcaggacttt tcagatgaac agaaatgatg tgatctttat
      541 cggatctgac ggaagagatg atattcagct tggagaggac gaaacaggca gaaggatcat
      601 caatgaaaat gaaaatcttt tcctgcagac aattgaagat gcagaagggc acctggaaaa
      661 aattcttgtc ctgacagaaa agaaaggtga aattactgat gatttcagtc tgctgaggat
      721 tcgttatatg ggtacagaca gtataatgct caatgaaaag cctgaattta tgcaggagtc
      781 cattgccctt cttcagaaag acaggtacga agaagttctt ctgactctgg atctgcataa
      841 aaacgatgaa atgggtgaat atgctactga gtttactgag ctggtcggaa agacagtatc
      901 tgcagttaaa aactacaggg ccggagtgaa catactgaat gaatttcatc tgaagcatcc
      961 agaagatgaa gaagcaattt atcttctagc tacatacttc tatcttctga aagattttca
     1021 taaagccgct gatttgggag aaattctgaa attaagaagt cctgaggatc caaagattct
     1081 tcgtcttctt gtggaaatct acactgcatc aggaaatatc attcgcgctc agaaaattct
     1141 gtccggtgtg cagagtacgc agtaaggaca aactgccgtg tgtccctacg gtccttgccg
     1201 attcatttca gatatttatc cagatcagca gaaatgatat ccatatgctt ctgaatttca
     1261 gcaggccaga caggaagact gtcattttcc cagtaccagt ctccgaccac attcagtata
     1321 ggctttgtat tgtaaaataa tgctttttta atgccaattt ggatatcttt gggttccaga
     1381 ggccggaatg gcttgtcgaa tgaactttct gttttgagct gtgattcttc aaattcatag
     1441 ttcattcctg gaaagaattc cttgaacttt ttgccggatt ttttccaagc ttctttgtat
     1501 tcattcattt ccggatacag attggaattt aatgattttt ggaccagaac agatatgcct
     1561 cgaacagagt cgctgtgaaa cattccgtac cggatgcact tgaactgttt tggaaactgc
     1621 agtgtgatct gttccatagt gatttttgta taagtcattg ttccgtaaaa aagcatcaga
     1681 ggcttgagcc acataaaacc ggaaaaccat acgcttaaac cgggcttcag aacctttgtc
     1741 agtttgagca ttgggatcac agaaaaattc agactgtcca gatactctgc cttgtctgca
     1801 tactgaaccg gtttccctac cattcctctg gctgggatat aaattaatga tttcaggtgt
     1861 ctgtctttca gtccttcaga aattctgctg atgctttctt ccgacctgag atcaatattt
     1921 cggaatgttt catctgcgtt ctgtacatcc ccttccttgc tggatgtgga aataatataa
     1981 gcgtctttat cttcagagcg gattgccgaa atagtctgct ctcctgaaac tgcactgccg
     2041 ccgatgacca gatagtagtt catagatata atacaaaatt gccagacggg atttttgtca
     2101 catttctttt ttctttaaac ttactcacct tacgccggcg gctgaagggc cgcctcttgc
     2161 tgttatgagg agacagggac ttgcctttac cagagactct tttggagcat tttgaatttg
     2221 tgcttcttga tactctcaag gcccaattct tttccaaact tatcatttaa actgacgtaa
     2281 tcttcttttg ttattttttt cttctttcca tctgtcgtct caacggacat tttcaccttg
     2341 ccgtcttcag taagggcatc aaagctccac tctggattat gactccagaa tatattccac
     2401 ttttctttgc ctaggtacca atgcttgata ccctcgaatt caggacgagg cagaacaacc
     2461 tccttggatg aggaatagat tctctgagca ttttcataaa accaggtata aggatcctct
     2521 gtgccctttg gtacctgaag aagcactgta tcttcaattg gaagataata gttgcccttt
     2581 ttgtcttttg cccaaggata aggaacgtca ttatttccca taggtgatac aaattcagtg
     2641 atttcggcta taggctggaa tgagaatatg catccgccta catatacata gtatccattc
     2701 tccatttgca gtagattagt atttccgtcc cactcagccc ctgcagttcc cagtgcaagc
     2761 gtagaaggtg tctcgggaga tttaccaata aaaattttct ccggatgata cttgaacaga
     2821 ggcttttttt caaaaaccag ctcagcctct gattcaaaac taggattatt gtccacagac
     2881 aaatccaatt tgtaaatcag cacctcgttt ttagaaagaa tttctacctt aaatggcctg
     2941 ccgccatttt catgtgtata atatactttt gtggacattt agcacctctg caaaattaca
     3001 ttctgtttaa gaattgacca ttaccttgtt caaggaagca ccgataagat caggaaaaat
     3061 atattctttg cagtcaaaaa gcagtcaagg aattttttca gggaaggaat aagatttccg
     3121 gaaactgtgt aaataaaaca gtttatattt agattagact tgttgaaaaa atcaagaaat
     3181 tatctttcat cagaagctaa atagggattt aggcagtttc agattttgcc gagccagctt
     3241 ccgaaccggc agccatcttc agaagtttgg cgaagggacg atagtccctt agcagcttcc
     3301 acgctagcga aacctccggt aggattaact cagcagcg
//
LOCUS       DAOQGJ010000189         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16754_length_3315_cov_2.723926, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000189 DAOQGJ010000000
VERSION     DAOQGJ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16754_length_3315_cov_2.723926"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..688)
                     /locus_tag="PK683_04150"
     CDS             complement(5..688)
                     /locus_tag="PK683_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HNH07669.1"
                     /translation="MILRDRLFERNPPERDAKKIYIFCEGRKREYQYFEYFEEIDSRI
                     NIIIYKLHPEENNSPNGLLKIAETCLIPSEENPEPEFFKDMNQEDEVWLVFDVDPDKS
                     NSRFPQILNVRQKCKEYGWNTALSNPCFEVWLYFHFFAEKPEMPDLNKCNSWKNHIDK
                     KLPGGFNSKKHPLFIEQAVVNAKQNFSLEGSFPVVGSTEMHYLGKTIYTLLKKKLNLA
                     KTSAIPQNP"
     gene            complement(663..2018)
                     /locus_tag="PK683_04155"
     CDS             complement(663..2018)
                     /locus_tag="PK683_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07670.1"
                     /translation="MLIRFVMENIYSFGERKEFNMIPYTRIRSQEHHIYKYDSVSVLK
                     MASIYGANGAGKSNLVKALLLLKSIVAGEELPPSVGASKFKFHPEDQKQVLAVEFIQE
                     KIPFYYALEISEGRILAEELYLSGLGKKEDVLLFERTSDSLRFTEEFESEEKNRILKT
                     VLTEEFLRPERPMIRILSNRDNNYFYYVKKAFSWFQNTLTVLNPDSRPQALAHKLDTD
                     KEFREFAEKIMCSYHIGVTALSSEKRNVSEIAGEGQDTVFLDFINSFRSSPNELIRAR
                     NTQGEELVFVKEDGEIFLKTLKMKHKGKGGLETSFSLSEESDGTVRLLDFVPAFHSLA
                     SSEKVFIIDEMERSIHPLLIRELVNKFSIDTESKGQLVFTTHESHLLDLEIFRQDEIW
                     FAEKDMNGSTDLYSLSDFKVHRTLDAQKGYLTGRYGAVPFLANLRELSWHAYDTQRQA
                     V"
     gene            complement(2582..3040)
                     /locus_tag="PK683_04160"
     CDS             complement(2582..3040)
                     /locus_tag="PK683_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07671.1"
                     /translation="MNVLLDPKIKGFLVFCFKELREVALIELRNGFKKQYDIVLDFYL
                     NRMDNSVHSRNIDLKKFDRIRKIARAYCEYEIHYSFSAPEEQKSFLENQSFQYFLISE
                     VDNVIRSYHQVRQDFYEKSRSGNFCRKKHIQDLIEVYRENSRVLEYGMIG"
     gene            complement(3037..>3315)
                     /locus_tag="PK683_04165"
     CDS             complement(3037..>3315)
                     /locus_tag="PK683_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07672.1"
                     /translation="RADVAFSEAYRGMADEIERVSREEVTIWARLTSWIYSIIFRDAG
                     HVKELQDSEEKHISAKAFKKMKNIFEPVEDHSFDNEEIMDHAQRKFKK"
BASE COUNT          998 a          697 c          587 g         1033 t
ORIGIN      
        1 aaaatcaggg gttttgtggg atggcggagg ttttagctag gttgagtttt ttctttaata
       61 gagtgtaaat tgtttttccc aagtaatgca tttctgttga acctacaacg ggaaaactgc
      121 cctctaagga aaaattctgt ttcgcattta caacggcctg ctcaataaag agagggtgct
      181 tttttgaatt aaacccgccc ggcaacttct tatcaatatg atttttccag gaattgcatt
      241 tattcaagtc aggcatttca ggcttttcgg caaaaaaatg aaaatacagc cagacttcaa
      301 agcagggatt actcaaagca gtgttccagc cgtattcctt gcacttctgc cggacattca
      361 gtatttgcgg aaaacgagag ttggatttat ccggatcaac gtcaaagaca agccatacct
      421 catcctcctg attcatatcc ttgaaaaatt caggttcagg attctcttca gaaggaatca
      481 ggcaggtttc tgcaatcttc aacagcccat tcggagaatt gttttcttca ggatgcagtt
      541 tataaattat tatattaatc ctggaatcaa tttcttcaaa atattcgaag tattgatatt
      601 cccttttcct gccttcgcaa aaaatataaa tcttcttagc atctctctct ggaggatttc
      661 gttcaaacag cctgtctctg agtatcataa gcatgccagc tcagttctcg aagatttgca
      721 agaaaaggca ctgctccata gcgtccggtc aaatatcctt tttgggcgtc caatgtcctg
      781 tgtaccttga aatcactcaa tgaatacaga tcagttgaac cgttcatatc cttctctgca
      841 aaccatattt catcctgacg aaatatttcc aaatccagca gatgagattc gtgcgtagta
      901 aaaacaagct gtcctttgga ttctgtgtct atggaaaatt tgtttaccaa ttctcggatc
      961 aaaagaggat gaatgcttct ttccatttca tcaataataa agactttttc tgacgaagcc
     1021 agactgtgaa aagcgggcac aaaatccaga agacggactg ttccgtcaga ctcttcactc
     1081 agactgaagg aagtctcaag cccccctttc cctttatgct tcattttcaa tgtcttcagg
     1141 aaaatttctc cgtcttcttt cacaaaaacc agctcctcgc cctgcgtatt tctggccctt
     1201 atcagttcat tgggagaact tctgaaactg tttatgaaat cgaggaagac tgtatcctga
     1261 ccttctccgg caatctcact gacatttctc ttttctgagg aaagagctgt tacgccaata
     1321 tgatatgaac acataatttt ttctgcaaac tccctgaact ctttatcagt atccagttta
     1381 tgtgcaagtg cctgaggacg ggaatccgga ttcaggacag tcaaggtgtt ttgaaaccag
     1441 ctgaaagctt tcttaacata ataaaagtaa ttattatctc tgtttgacag aatcctgatc
     1501 atcggtcttt caggccgaag aaattcctcc gtgagaacag ttttcagtat gcggtttttt
     1561 tcttcacttt caaactcttc tgtaaaacgc agactgtcag aagttctttc aaaaagcaga
     1621 acatcttcct ttttcccaag accggacaaa tacagttcct cggcaagtat tctgccttcg
     1681 gaaatttcta aagcatagta aaaaggaatt ttttcctgta tgaactcaac tgcaagcacc
     1741 tgcttctggt cttccggatg aaatttgaat tttgatgctc cgacagaagg aggaagttcc
     1801 tccccggcta caatgctttt gagcagaagc aatgctttga ccaagttcga ttttccagcc
     1861 ccatttgcgc cgtagatcga agccattttc agaacagaaa cagagtcgta tttatatata
     1921 tgatgttcct gtgatcggat tcgggtatat ggaatcatat tgaattcctt tctttcaccg
     1981 aatgaataaa tgttctccat tacaaatcga attagcatat aacctttgcg tgagtatttc
     2041 accctatatc ctcattttcg gtagatttcc caagcaagtc aagttgattt tgaaatttaa
     2101 cgtgaaattt tcacttcata ttcaaatttt aaactgtcct ttctatgctt gcaatataac
     2161 cccatgcaag attgaaccga tccaaaaaat tggatacagc gcaattacac aatgaactga
     2221 ggcaggagct gctggaaaga aaacaaaaag ctcttctgga tttagaacaa ggaaaagtaa
     2281 tttcagaaga agaattgtac ctggaaatgg aggggcttgc tgggaaataa aattgtcttt
     2341 tctgacggtg caagacagga tatcaaagaa tctatagagt attacgaaaa cagagaaact
     2401 gggcttggaa ataaatttct atctgacata aaagcaaaaa atcaaaacat taaaaataaa
     2461 actgagaaaa atcctgccga tactgacggg gttcgaaaat ctaaactgaa aaaattatcg
     2521 cagtctggca tattgcaagg ctcccgtttt ctggaaaaga cagaattgaa tagataaacc
     2581 ctcacccgat cattccgtat tcgagcacac ggctgttttc acgatagact tcaatcagat
     2641 cctgaatatg ctttttcctg cagaaatttc ctgaacggga cttttcataa aaatcctgtc
     2701 tgacctgatg ataacttctg atcacattgt ccacttcgga aatcagaaaa tactggaagg
     2761 actgattttc caaaaatgat ttctgttctt caggagctga gaatgaataa tgaatttcat
     2821 attcacagta agccctagca atttttcgaa tcctgtcaaa ttttttcagg tcaatatttc
     2881 tgctgtgaac agagttatcc attctgttca gataaaaatc cagaacaata tcatactgtt
     2941 ttttgaatcc gtttctaagt tcaatcaagg caacttctct cagttcttta aaacagaaaa
     3001 ccagaaaacc tttaattttt ggatccagaa gaacattcat ttcttaaact tcctttgggc
     3061 atgatccatg atttcttcat tatcaaatga atgatcttcc accggttcga aaatattctt
     3121 catttttttg aatgctttcg cagaaatatg tttttcttcc gaatcctgaa gttcttttac
     3181 gtgaccggca tctctgaaaa tgatactgta tatccagctc gtaagtcttg cccagatcgt
     3241 aacttcttct cttgagacac gttctatctc gtcggccatt cctctataag cttcagaaaa
     3301 tgcaacatca gccct
//
LOCUS       DAOQGJ010000190         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16850_length_3304_cov_4.000000, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000190 DAOQGJ010000000
VERSION     DAOQGJ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16850_length_3304_cov_4.000000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..2507
                     /locus_tag="PK683_04170"
     CDS             249..2507
                     /locus_tag="PK683_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNH07673.1"
                     /translation="MNSDWYANLLEAQQQVIEKIALGHSLSECLELICLLIESTLNEP
                     EARSSILLLEGYRLWTGAAPNLPKSYCKAIDGLMIGPKVGSCGTAAFTKKQVIVENIE
                     TDPLWEEFKEIALFHQLYSCWSNPILSSANEVLGSFAIYYTRPKYPEKHHLDLIARFT
                     HLSGLAIEKARLSKREADLTLELMNNNRKFSAFTSVMPDLAMIIDEDGKYVDVYGGDQ
                     TMLYDSVHNLLGNYIKDILPPQKASDIMNVIQRAIQTGKVQIFEYRLPVPKGILVFEG
                     RVAAIEHYLPNEPEKRHVLWMARDITENKRAQAEIENLAFYDSLTHLPNRRLLIERLQ
                     LLIHRSQHSRIVSSLLYLDLDDFKRINDTLGHSVGDLLLVSIADRLKPLLEGIGIFAR
                     IGGDEFVILLEGMELDLDIISEASVSIASKIIEAFSLSFKIQKGEFRIGTSIGISIIH
                     GENMNADEVLKRADAAMYISKQKGGNCFTFFDPSLQKILDSRLEVEQGIIRAVKEGEF
                     CAYFQPQVSTDNKVFGAEALIRWIHPEKGIISPLEFIPIAEQFGLIHQLQNIVLHDAC
                     RLLNMLTERGLIDDDFTVSVNISAIQFRNPELKKALNRTMENHRLSPRRIKLEITEST
                     LIDNIDHTLLQMKLLKNEGYAFSIDDFGTGYSSLTYLQTFPIDELKIDKSFVDKIQAS
                     EVGTAIVDAIIALSQHMGFEVIAEGVEHKSQVDILSKRGIKAMQGYFFGRPMTADKLA
                     EWLQSMRDEIPA"
     gene            complement(2525..>3304)
                     /locus_tag="PK683_04175"
     CDS             complement(2525..>3304)
                     /locus_tag="PK683_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH07674.1"
                     /translation="VDVQHKRGDKTREIVEAPIVISSTGAYHTYTELLKDYAPASYIN
                     TIKSFSKGNSAVTLFIGLNKDPSLLGFKGENNWIYESYDHDALADRTEKALDGKAEFC
                     YLSFPSLKNTQAKAHTAEIISFMKYDLFKIWESSRWKKRGMEYDHLKERIADGLLDLV
                     EKHYPGFKDMVEYTELSTPLTYLDLGGRPRGEFYGLPAVPEKYRIKWLGPKTPLKNFY
                     MSGSDVMSLGIMGAMMGGVAAAACVLEPAGFPKIMKAVDRK"
BASE COUNT          954 a          668 c          713 g          969 t
ORIGIN      
        1 tgactgctgt tttccagata acctgccccg cttccgaaaa ctctcctgcc actgcacaaa
       61 atctcattga ttttgaaaaa tcgaattttc agattcgaaa aattttcttg aattttataa
      121 tatgggaggg tatttatgat gaaaaactat tgacggattt tgaaaaatat tcagtatcat
      181 tgaaaaacat catgcataca cgaaatgaaa aagactgatt ttgcatattc attgcggctc
      241 ataaaataat gaactcggac tggtacgcca atctgctgga agcgcagcag caggtcatcg
      301 aaaaaattgc acttgggcat tctctttcgg aatgtctgga attgatatgc ctgcttattg
      361 aatctactct gaatgaacct gaagccagaa gttccattct gctcctggaa ggataccgtt
      421 tatggactgg ggctgctccg aatctgccta aatcatactg taaggcaatt gacggattaa
      481 tgatcggtcc aaaggtagga tcctgcggta ctgccgcatt tacaaaaaag caggtcattg
      541 ttgagaatat tgaaacagat cctctctggg aggagtttaa agagattgcc ttgtttcatc
      601 agctctattc ctgctggtca aatccaattc tctcctcagc caatgaggta cttggaagtt
      661 ttgccattta ttatacaagg ccaaagtatc ccgaaaagca tcatcttgat ctgattgcca
      721 ggtttacgca tctttccggc cttgcaattg aaaaagcgcg tctttccaaa agagaagccg
      781 atctgacgct tgaactgatg aacaataaca gaaagttttc agcttttaca agtgtgatgc
      841 cggatctggc tatgattatt gacgaggacg gaaagtatgt agatgtctac ggcggtgatc
      901 agacaatgct ttacgacagt gtacataatc ttctgggaaa ttatataaag gatatacttc
      961 ccccccaaaa agcatctgat attatgaatg tcattcaaag agccattcag actgggaaag
     1021 tgcagatatt tgaataccgg cttcctgttc ctaagggcat acttgtattt gaaggaagag
     1081 ttgctgcgat tgagcattat cttccgaatg aacctgaaaa aagacatgtg ctttggatgg
     1141 cacgcgatat cactgaaaac aagagagcac aggctgagat tgaaaatctg gcattttatg
     1201 attctcttac gcatcttcca aacagaagac ttctgattga acgccttcag cttctcatcc
     1261 acagatcaca gcatagcaga atcgtcagct cacttctata tctggatctc gacgatttca
     1321 aaagaattaa tgataccttg gggcattcgg tgggagatct gcttttggtc agtattgctg
     1381 atcgattgaa acctcttctg gaaggaatcg gaatttttgc acggattggc ggggatgaat
     1441 ttgtcattct tcttgaagga atggagcttg atctggatat tatctcagaa gcatcagtat
     1501 ccattgcatc aaagattatt gaggcctttt ctctgagttt caagattcaa aaaggtgaat
     1561 tcaggattgg tacaagtatc ggtatcagca tcattcacgg agaaaatatg aatgcagacg
     1621 aggtactgaa acgtgctgat gctgcaatgt atatttcaaa gcagaaagga gggaactgct
     1681 ttactttctt cgatccttct cttcagaaaa ttctggacag cagacttgag gtggagcagg
     1741 gaattatcag ggctgtcaag gaaggagagt tctgtgccta ttttcagcct caggtcagta
     1801 ctgacaataa ggtgttcggt gccgaagcac tcatcagatg gattcatccg gaaaagggca
     1861 tcatatcacc tttggaattt attccgattg cagaacagtt tggactgatt catcagctcc
     1921 agaatattgt cctgcatgat gcgtgcaggc ttctgaatat gcttacggaa agaggactga
     1981 ttgacgatga ttttacagta tcagtcaata tcagtgctat ccagtttcga aatccggaac
     2041 ttaaaaaagc actgaacaga acaatggaaa atcaccgtct ttctcctcgc agaatcaaac
     2101 tggaaattac tgaaagtaca ctgattgaca atatagacca taccttgctt cagatgaagc
     2161 ttctgaaaaa tgaaggttat gcattttcca ttgacgattt tggaacaggt tattcctcac
     2221 tgacttacct gcagacattt ccgattgatg agctgaagat tgataaaagc tttgtggata
     2281 aaattcaagc cagtgaagtg ggaacagcga ttgtggatgc gattatagca ctgtctcagc
     2341 atatgggatt tgaagtgatt gcagagggcg tggaacataa gtcacaggtg gacatacttt
     2401 ccaaacgcgg catcaaggcg atgcagggtt attttttcgg caggccgatg actgcagaca
     2461 aacttgcaga atggctgcag tctatgcggg atgaaattcc ggcataatga aaaaatatct
     2521 gtttttattt cctgtcgact gccttcatta ttttaggaaa accggcaggt tccagaacgc
     2581 aggctgccgc agcaacacct cccatcattg cccccattat tccaagagac attacatctg
     2641 agccggacat ataaaaattc ttcaaaggag ttttcggtcc aagccatttg attctgtatt
     2701 tttccggaac agcaggcaga ccgtaaaatt ctcctctggg tctgcctccc agatcaagat
     2761 atgtcagagg cgttgagagt tcagtgtatt ccaccatatc cttgaatccc ggataatgtt
     2821 tttcaaccag atccaaaagt ccgtcagcaa ttctttcttt cagatgatca tattccatcc
     2881 ctcttttttt ccatctgctg ctttcccaaa tcttgaatag atcatacttc ataaaagaga
     2941 taatctctgc agtatgtgct tttgcctgtg tgttcttcaa tgaaggaaac gagaggtagc
     3001 agaattctgc ttttccgtcc agtgcctttt ctgttctgtc ggcgagagca tcatgatcat
     3061 aggattcata gatccagttg ttttctccct tgaatccaag cagagaagga tccttgttca
     3121 atcctatgaa gagagtgact gcactgtttc ctttggaaaa acttttgata gtatttatat
     3181 aggatgccgg ggcataatct ttcaacagtt ctgtataagt atgataagcc ccagtggatg
     3241 agatgactat gggtgcttcc acaatttccc tggttttgtc gcctctctta tgctgaacat
     3301 ccac
//
LOCUS       DAOQGJ010000191         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_16905_length_3299_cov_3.164303, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000191 DAOQGJ010000000
VERSION     DAOQGJ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16905_length_3299_cov_3.164303"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PK683_04180"
     CDS             complement(<1..1090)
                     /locus_tag="PK683_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[cytidine(C)-cytidine(C)-adenosine (A)]-adding
                     enzyme"
                     /protein_id="HNH07675.1"
                     /translation="MKKISTEQFSLIDRKNQENLEKITSVLNEAGYECYLIGGSVRDM
                     LMNRVPHEYDLAVSAEPEEVKKLFRKVIETGLKHGTVTVLIDGFPYELTTFRKDIDYI
                     DGRHPERVEFGATLSEDMKRRDFTMNAIALNIRTGDLTDENEGISDIHKKIIRTIGNA
                     VERFTEDGLRPIRAIRFQSSLDFTIEEETYKAIAKTRHITAKISTERFHDEFIKILKT
                     EKPGKAVIELIRNRIFSLFWGDISEKETESVMNLDSLPNVLNLRLAYFYDTQTDSEKK
                     WEILENSMKRLRFSNEKIRESSVYIRSVNEFQNIEYMDDYGRRKFLSFLSMNIGKKNA
                     PVIIENLKHWWLLKNVRSHETLAENLKKE"
     gene            complement(1087..1389)
                     /locus_tag="PK683_04185"
     CDS             complement(1087..1389)
                     /locus_tag="PK683_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07676.1"
                     /translation="MSVKKEKLIKVGKWTEGIHNSNEKRIKKLLKEILPNYKEILWDE
                     ASFQRFYIEVLNSLPSRYKQKNSIELNGRLKEEILKEAIIEKLEEMEEQILLESSL"
     gene            complement(1380..1790)
                     /locus_tag="PK683_04190"
     CDS             complement(1380..1790)
                     /locus_tag="PK683_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNH07677.1"
                     /translation="MVSEKSLESLKKNKLKVTRNRIAVLDLLKSSDKPMTHSDIMEGL
                     SKEGHWDRVTIYRTLSEFAEKQIVQIIHSDERLTYFELKDDPPEHGHLICDSCGKLYC
                     LKDEMFHLRLEGNLKSFQIKRIEIFIRGLCKSCQ"
     gene            complement(1790..2128)
                     /locus_tag="PK683_04195"
     CDS             complement(1790..2128)
                     /locus_tag="PK683_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07678.1"
                     /translation="MRILFYLILLLSFCSRNPAEKEIKKAISSGDFLTAAKFCADYKE
                     KNLEKECAEQKEKSEEEINRILSEKQNLPFTKLIVDRELSRKVRELLKKDIKLGIQYR
                     QIWEETVEKR"
     gene            complement(2107..3162)
                     /locus_tag="PK683_04200"
     CDS             complement(2107..3162)
                     /locus_tag="PK683_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07679.1"
                     /translation="MDELIDNIEEKITLALQSVPREMAQDVIENKIQDAYAIKVLVEL
                     ENKNGILIAVFSSVNMKMLSMHSAAGFSSHLKKVWTFNPWKEFAGDFVTALKAGGIEN
                     GLSDRVGRSVYRALTVQNVSALVPNLDLGQKDGIISMITAELVKEMKISKISIQLDFE
                     KVNSVGYRTENPINGLWNEVRNPFEEQVQEAKPVQEKKKSSGYEFIDAIRERYDSIAR
                     CSSVISPLAGMEFKDLKPGQPILVQCPFKTTDEKIMARRLGAVNDKGINSPIQAKFID
                     LVHTEKEYHILAEGPNKILIHIIEERPVRIAVPKEFRTSKYRAFEKEDTSTANSLKII
                     LIVLTVVVIGLCVFYFI"
BASE COUNT          805 a          716 c          600 g         1178 t
ORIGIN      
        1 gttctttttt cagattttcc gcaagtgtct catgggaacg tacgtttttt aacagccacc
       61 agtgtttcag gttttcaata atcacaggag catttttttt gccgatgttc attgagagaa
      121 aggacagaaa tttcctcctt ccatagtcat ccatgtattc aatgttctga aattcattta
      181 cagatctgat atagacggaa gactccctta ttttttcatt tgagaacctt agtctcttca
      241 ttgagttttc gagtatctcc catttttttt ctgaatcagt ctgagtgtca taaaaatagg
      301 ccagacggag gttcagaaca ttcggaagac tgtccagatt catgactgat tcagtttcct
      361 tttctgaaat gtctccccag aaaagggaaa agattctgtt cctgatcagc tcaatgactg
      421 cttttcccgg tttttcagtt ttcaaaatct taatgaattc atcatgaaat ctttcggtgg
      481 atatctttgc tgtgatatgc cttgtcttcg caattgcctt ataagtctct tcttcaattg
      541 taaagtcaag agaggactga aaacggatag cccggatagg acggagtccg tcttcagtaa
      601 atctttccac tgcatttccg atagtcctga tgatcttttt atgaatatca gagatccctt
      661 cgttttcatc cgtcagatcg cctgtccgga tattgagagc aatcgcattc attgtaaaat
      721 ctcttctttt catatcttca gaaagagttg cgccgaattc cactctttca ggatgtctgc
      781 cgtctatata atcaatatct ttacggaatg tcgtgagttc ataaggaaag ccgtcaataa
      841 gaactgttac tgttccgtgc ttcagtccgg tttcaatgac ctttctgaaa agtttcttca
      901 cttcttccgg ttccgctgaa actgcaagat catattcgtg cgggaccctg ttcataagca
      961 tatctcttac ggaaccgcca atgagataac attcgtaacc tgcctcattc agaacggatg
     1021 tgattttttc cagattctcc tgattttttc tgtcaatcag agagaactgt tctgtactga
     1081 tttttttcaa agagatgatt ccagaagaat ctgttcttcc atctcttcca gtttttctat
     1141 gatggcctct ttcagaattt cctctttcag tctgccgttc agttcgatgg aatttttctg
     1201 cttgtatctg gaaggaagac tgttcagaac ttcaatataa aatctctgaa aactcgcttc
     1261 atcccagaga atttctttgt aattcggcag aatttctttc agaagctttt taattctttt
     1321 ttcattagag ttatggatgc cttctgtcca tttccccacc ttgatcagtt tttctttttt
     1381 tactgacatg atttgcagag cccgcgtata aaaatttcta ttctttttat ctgaaaactt
     1441 ttcagattgc cttcaagtct taagtgaaac atttcatcct tcagacagta cagcttgccg
     1501 caggaatcgc agatcagatg cccatgttcc ggaggatcgt ccttcagctc aaagtaggtg
     1561 agtctttcgt ctgaatgaat aatctgcaca atctgttttt ctgcaaactc gctcaatgtt
     1621 ctgtaaattg tgactctgtc ccagtgacct tccttggaaa gtccttccat aatgtcggaa
     1681 tgggtcatcg gtttgtccga cgatttcaga agatcgagca cggcaattct gttccttgtc
     1741 actttcaatt tatttttttt cagtgattcc agcgattttt cggagaccat tatctttttt
     1801 ctacagtctc ttcccagatc tgtctgtact gaattcccag tttaatgtct ttcttcagca
     1861 gttctctgac tttcctgctc agttccctgt ccacaatcag tttagtaaaa ggaagattct
     1921 gtttttctga gagaatcctg ttgatttctt cctcagactt ttctttctgt tctgcacatt
     1981 ccttttccag attcttttcc ttataatcag cgcagaattt tgcagctgtc agaaaatcac
     2041 ctgaactgat ggctttcttt atttctttct ctgcaggatt tctgctgcag aaagagagaa
     2101 ggagaatcag ataaaataga atacgcatag gccaattacg accacagtca gtacaatcag
     2161 tatgattttc aatgaatttg ctgtggatgt atcttctttt tcaaatgccc tgtacttgct
     2221 tgttctgaat tcttttggaa ccgcaatccg gacaggtctt tcctctatga tgtgaatgag
     2281 tattttattc ggtccttctg caaggatatg atattccttc tcagtatgaa ccagatcaat
     2341 gaattttgcc tggatcggac tgttgattcc tttgtcattg actgcaccaa gtcttcttgc
     2401 cataatcttt tcgtcagtgg ttttgaaagg gcactgaact aaaatcggct ggccgggttt
     2461 caggtccttg aattccattc cggcaagagg tgaaattact gaactgcatc tggctatgga
     2521 atcatatctt tcccgaatcg catcaatgaa ttcatatccg gatgatttct ttttttcctg
     2581 aacaggtttt gcttcctgaa cctgctcttc aaatggattt ctgacctcat tccatagccc
     2641 gttgatcgga ttctctgttc tgtacccgac tgaattcact ttctcaaaat caagctgaat
     2701 gctgatttta ctgattttca tttccttcac aagttctgca gtgatcattg aaatgattcc
     2761 gtctttctgg cccagatcca gattaggaac aagggcagaa acgttctgaa cagtcaatgc
     2821 cctgtaaacg gatcttccta ctcggtctga gagtccgttt tcgattccgc ctgccttcag
     2881 agcagtgaca aagtcgcctg caaattcttt ccagggattg aatgtccata ctttttttaa
     2941 atgagaagag aatcctgccg cggaatgcat agaaagcatc ttcatattga ctgaagaaaa
     3001 tacggcaatc agaattccat ttttgttttc cagttcaacc agaaccttaa ttgcgtatgc
     3061 atcctgaatt ttattttcta tgacatcctg tgccatctct ctgggaacag actgaagtgc
     3121 cagggtgatt ttttcttcta tattgtcgat gagttcatcc atcagattcg aaattccttc
     3181 ctaagatctt ccttagggat attttttact tttagaattt cttcaaactt tattctggag
     3241 ggtttgtatc cttctccgat cggaatttct acctttgcca ctgttgagct tggattgtg
//
LOCUS       DAOQGJ010000192         3280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17024_length_3280_cov_3.172403, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000192 DAOQGJ010000000
VERSION     DAOQGJ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17024_length_3280_cov_3.172403"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1874
                     /locus_tag="PK683_04205"
     CDS             <1..1874
                     /locus_tag="PK683_04205"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNH07680.1"
                     /translation="FFILSIGPLLFVIILGFALKFSDFVRHSHLYSVSMDSKNYLWIA
                     GERGTLLKIDQSGKKYTKLSDLKIDFENMECLDIKAEKQVSACDAPNLQKEDFIKLRS
                     KSKVLYAASKKGILLISDDAGESWKISQFLNTEFKDVAVSDDKRIFIITESGEVVRYA
                     RDGLFEKIVLKTAEANSYALRVSIQDRKLGFILDNSGRIWISRDGGNNFESRKLSDKR
                     LEDMEVLDEKTFIVVGEKGGIFRTTDGGENWENVSHRNITFTKVWHFSDGKDRLLLVL
                     NNFDQILVSTDLGDNWKKSYSPENGSLQGITAINPRLGFKTVNPEEEGGLITNILQTE
                     GEKSQEKVKEAAGDLLAVGEFNKIAVGDIVDGQVVWKTLSGGDKMFSMYSIIRFIIPL
                     AAIWTFFFTLYSLIPNAKVPIRPAVVGSFLTSAIFLLFIYGYSIYVRSFSDSTLVIYR
                     ALAAIPLLLLSIYCISLIILLGAEITATLQYRDRYADDANFLEAEEQEKNYSFYKSLK
                     LLLILYGNQKNRKQPVKLHSLQLELKMSGLDFSRVLEALEKEKLIVVGDEKDVVPSLS
                     SADLDVYEIMKIIAKTSMDIPPDEVSEKLAGILKNEIVKIENYSKENLKKIRLADLV"
     gene            1875..>3280
                     /locus_tag="PK683_04210"
     CDS             1875..>3280
                     /locus_tag="PK683_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNH07681.1"
                     /translation="MEERKYDYDYIVIGSGFGGSVSALRLAQKGYKVAVLESGKRWKS
                     PEHPKTNFNIRKFLWMPNLFCYGIQRLNLLNDVLILSGAGVGGGSLVYANTLYVPPQK
                     FFDQEIVKSMGGKEELLPFYEIAQKMLGVVENPKIWEVDEYMKQTAAEFGVEHTFKRT
                     PVGINFHDKDKEDPYFSGEGPLRDGCNFCGGCMIGCRNNAKNTLDKNYLYFAEKLGVY
                     VIPETKAVEVKPLSEDGSEGYEIRTRTTTGLFGFPKRKFTAKGVVFSAGVLGTMGLLM
                     KMKEKRTLPKLSSMLGKIVRTNSESILGVTALDSKADYSKGIAITSSIYPDEHTHIEP
                     VRYPAGSDAMAALAVNTLVDGGGKVPRQFRFLISFVSSFIRHPLRNLRFLIPYGFAKR
                     SIILLVMQTVDNSIDIVRKRRLIWPFWKTLSSKQKTGEKIPTYIPIANEFARKLAKRI
                     NGVARSSINEVLLDVPTTA"
BASE COUNT         1002 a          614 c          749 g          915 t
ORIGIN      
        1 atttctttat actttcgata ggcccgcttc tttttgtgat catccttgga tttgcattaa
       61 aattctctga ttttgtgaga cattcgcatt tatattcagt ttccatggat tccaaaaact
      121 atctgtggat tgcgggagaa agaggcacac ttctgaagat agatcagtcc ggtaaaaaat
      181 atactaaact ttctgatctg aaaattgatt ttgaaaatat ggaatgtcta gatatcaagg
      241 cagagaaaca agtcagtgcc tgtgatgctc caaatcttca gaaagaagac tttatcaaac
      301 tgagaagcaa gtcaaaagtt ctttatgctg catcaaaaaa gggaatcctg ctgatatccg
      361 atgatgcagg tgagtcctgg aaaatttccc agtttctgaa tacagagttc aaagatgtgg
      421 cggtgtcgga cgataaaagg atctttatca ttactgaatc aggcgaagtg gtccgatatg
      481 ccagagatgg gctttttgaa aaaatagttc tgaagacagc agaagccaat tcttatgccc
      541 ttcgagtcag tatccaggac aggaaactgg gattcatttt ggataattca ggcagaatct
      601 ggatttccag ggatggagga aacaattttg aatccaggaa actctctgac aaaagacttg
      661 aggatatgga agttctggat gaaaagacgt ttattgtcgt tggagaaaag ggcggaatat
      721 tcagaacaac agacggcgga gaaaactggg aaaatgtatc tcacaggaat attaccttta
      781 caaaggtttg gcatttttct gacggcaagg acagacttct tcttgttctg aataattttg
      841 accagattct ggtttccact gatttgggag acaactggaa aaagtcctac agtcctgaaa
      901 acggaagtct tcagggaatt actgccatca atcccagatt gggttttaaa acagtgaacc
      961 cggaagaaga agggggcctg attaccaata tccttcagac tgaaggtgaa aagtcgcagg
     1021 aaaaagttaa ggaggcagca ggagatcttc ttgctgtcgg agagtttaat aagattgcag
     1081 tgggagatat tgttgacgga caggttgtgt ggaagactct cagcggaggg gataaaatgt
     1141 tctccatgta ttccattatt cgtttcatta tcccgcttgc tgcgatctgg acatttttct
     1201 ttactcttta ttctcttatt cctaatgcaa aggttcccat tcgtcctgca gttgtcggtt
     1261 catttcttac aagtgcaata tttcttctgt ttatttatgg atactccatt tatgtaagat
     1321 ctttttccga ttcgacactt gtaatttaca gggctcttgc tgcaattcct cttctgcttc
     1381 tttccattta ctgtatatca cttattattc tgctgggtgc cgagattact gccactcttc
     1441 agtacagaga cagatatgca gacgatgcaa attttctgga agcagaagaa caggaaaaaa
     1501 attacagctt ttacaaatct ctgaaacttc tcctgattct gtatggaaat cagaagaaca
     1561 ggaagcagcc tgtcaaactg cattcacttc agcttgagct gaaaatgagc ggattggatt
     1621 tttctagagt attggaagcg cttgagaagg aaaaactcat tgtagtcggg gatgaaaaag
     1681 acgttgttcc ttcactttct tcagcagatc tggatgtata tgaaatcatg aaaattatag
     1741 caaaaacttc catggatatt cctccggatg aagtttcaga gaaacttgcc ggcatcctga
     1801 aaaatgaaat tgtaaaaatc gaaaattatt caaaagaaaa cctgaaaaaa atcagactgg
     1861 cggatcttgt ttaaatggaa gaaagaaaat acgactatga ctatattgta atcggttccg
     1921 gtttcggagg aagcgtgtcc gctttgagac tcgcacagaa gggatacaag gtggctgtac
     1981 ttgaatcggg aaaacgatgg aagtcgcctg agcacccaaa gacaaatttt aatattcgga
     2041 agttcttatg gatgccgaac cttttctgct acggcatcca gagactgaat cttctgaacg
     2101 atgtgctgat tctatcaggt gcaggcgtag ggggaggaag tcttgtatat gcgaatacgc
     2161 tctatgtccc tcctcagaag ttttttgatc aggaaattgt aaagtctatg ggcggaaagg
     2221 aagaacttct tcctttttac gaaattgctc agaagatgct cggtgttgtg gaaaatccta
     2281 agatctggga agtggatgaa tatatgaaac agactgctgc cgaattcggt gtggaacata
     2341 cattcaagag aactcctgta ggtataaatt ttcatgataa ggacaaggaa gatccttatt
     2401 tcagcgggga aggtcctttg agagacggat gcaatttctg cggcgggtgc atgatcggat
     2461 gcagaaacaa tgcaaagaac actctggaca aaaactatct gtactttgct gaaaaactgg
     2521 gagtctatgt tattcctgaa acaaaagccg tcgaagtaaa gccgctcagt gaggacggat
     2581 cggaaggtta tgaaatcaga accagaacaa cgactggact ctttggattt cccaaaagga
     2641 agttcactgc aaagggagtc gttttttctg caggagttct cggaactatg ggacttctga
     2701 tgaaaatgaa agaaaaaaga actctcccta aactttcttc catgctcggt aagatagtcc
     2761 gaacaaacag cgaatcgatt ctgggagtga ctgcattaga cagcaaggcg gactattcca
     2821 aaggaattgc aatcacttca agcatttacc cggacgaaca tactcatatt gaacctgtga
     2881 gataccctgc aggatcagat gcaatggcgg cacttgcagt aaataccctc gtggacgggg
     2941 ggggaaaggt ccctagacag ttccggtttc tgatatcatt cgtttcctct ttcatcagac
     3001 atcctttgag aaatctccgt ttcctgattc cttacggttt tgcaaaacgt tccatcatcc
     3061 ttcttgtaat gcagacagtg gacaacagca ttgatattgt caggaagaga agactcatct
     3121 ggcctttctg gaagacactc tcctcaaagc agaaaacggg tgaaaagatt cctacataca
     3181 ttccgatagc aaatgagttt gcgagaaaac ttgccaaaag aattaacggt gtggcaagaa
     3241 gctcgatcaa cgaagtgctt ctggatgtcc ccactacagc
//
LOCUS       DAOQGJ010000193         3278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17037_length_3278_cov_2.547937, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000193 DAOQGJ010000000
VERSION     DAOQGJ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17037_length_3278_cov_2.547937"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..509)
                     /locus_tag="PK683_04215"
     CDS             complement(291..509)
                     /locus_tag="PK683_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07682.1"
                     /translation="MQSMLQTQSANALILDPSINSADISTFTGFSGTNKWNDGILAPN
                     GKIYAIPRDATSILIIDPKSAGTWDMSV"
     gene            complement(1030..1344)
                     /locus_tag="PK683_04220"
     CDS             complement(1030..1344)
                     /locus_tag="PK683_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07683.1"
                     /translation="MNNPKFIIVLRIIILLVVIPSFLLSLLFVMERVQEKISFLSWMD
                     FSFVFLPFLVAGAGLFTSVWSLFRKREFSLLELAIYIFSFLAVILNFIILYIVISLAE
                     NG"
     gene            complement(1350..1664)
                     /locus_tag="PK683_04225"
     CDS             complement(1350..1664)
                     /locus_tag="PK683_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07684.1"
                     /translation="MTDPAFVLYLRISILLISLPVFLISFLLILVRIREKGFSFSLPE
                     AFIMLLPFCAAGAGIFFSAYGLVLKSSWKFTELIIDCLSISAAFIIAFVLYTVIRQSQ
                     EG"
     gene            complement(1657..2637)
                     /gene="rsgA"
                     /locus_tag="PK683_04230"
     CDS             complement(1657..2637)
                     /gene="rsgA"
                     /locus_tag="PK683_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNH07685.1"
                     /translation="MNLEKGQYVITRIYGAYYEIYSPETGLKRAVLRGKLRLSGSTER
                     NPLTPGDIVNADAGANSGDDYKIMSGVERKNALLRKSRDGDAQALCANIDYAAILASL
                     ANPETKPGFIDRCLASCHFAGITPLIIFTKCDLPDADFIEEKTSVYRELGYNVMTVSA
                     ATGEGIEELRLKLKDKVTFLAGISGAGKSSLVNAVSGENMMKVGEVSHSSGKGKHTTT
                     NSHMIFLPDGITLIDSPGIKEWGILHMKKGDIVSSFPEFFKIRKKCPITGCCNLQDGC
                     LLTDKMDEKHISYERLISMEAMLESVDVPHRIRTGNFKSGKVRRREKNYD"
     gene            complement(2634..>3278)
                     /locus_tag="PK683_04235"
     CDS             complement(2634..>3278)
                     /locus_tag="PK683_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH07686.1"
                     /translation="AEEISFSYTGKEPYIINKFSLSVGNGDRICIIGKNGKGKSTLLK
                     ILAGEFKPVSGTVNKHPVLKEGYFGQTNKLDLNDSNTIVEEIMSADKDCNEGKARNIA
                     GGLMFSGDSALKKIKVLSGGEKSRVLLGKILVTPCSLLFLDEPTNHLDMQSCDALIEA
                     IDQFEGSVIMVTHNEMHLRAVATKLVVFDNDMISIYDGGYDDFLADVGWSDEDL"
BASE COUNT          940 a          706 c          656 g          976 t
ORIGIN      
        1 ctctcaaaag ctttaccgca gaaatctcag caaaaaatct gaatcagaga aatctgacag
       61 ccgttctccg ttttctttct tcttttatcc agtttgtcgg cacgaagccg ttctctgaat
      121 aagtgctgtt tttctgattc atagcaagct gttaaactgc ggatacctct aatgattcgg
      181 aaaaattata gattttatgc aatatattct ttttgcttaa agtgagttta taatatttat
      241 cacttagttt aaactgatat tctttagttt ttattaaaat atgaactcaa tcaaacagac
      301 atatcccagg ttcctgccga ttttggatca ataattaata tgctcgtcgc atctctggga
      361 atggcataaa ttttgccgtt aggtgctaaa attccgtcat tccatttatt tgttccagag
      421 aaacccgtaa aagtgcttat gtctgcagaa tttatggatg gatcgagtat cagagcattt
      481 gcggactgag tctgcagcat cgactgcact attgaccaat tagaaggttg aattgcttta
      541 tcggtgctta tagttacacc gattgagatt ggagcattta cacttccagc tgaaccgcct
      601 gctgtaattg tatattttga tgatgactgc aatacagtcg gtgcagttac agtttgagat
      661 gctgtaaatt tagccagaaa tatccactcc agagaagatc tatgcaatgg gggattacag
      721 cttagaaaaa ccagtataac aagatattca tagttcagga ttacctgaca agcactgtct
      781 cgaaagtttt gcatatttaa tccccgctga ttcagcaatt tatctgctgc tggaacgcct
      841 gtcggccggt aatcagcata aaagaatcaa cagcacattg gttgaaagtc agctccggct
      901 gacgctgcag gcggggtgtt ttgggcagga acgtctgccg gcggcttaag ggatgatgta
      961 aatcaggtat ttttgagttg cagattttcg gtcggcatat ttccaaatga cagagtccaa
     1021 ttctctgaat tacccgttct cagcaagact tatgactata tacaatataa tgaaatttag
     1081 aataaccgca agaaaggaaa aaatgtagat tgcaagctcc agaaggctga attctctttt
     1141 tctgaaaaga ctccagacag aggtgaacag accggctcct gccacaagga agggaagaaa
     1201 tacaaaggag aaatccatcc aggaaaggaa ggaaattttt tcctgaactc tttccattac
     1261 aaaaagaaga gagagcagaa aagagggaat cactacaaga aggattatga ttctaagtac
     1321 tattataaat ttggggttat tcattgcatt catccttcct gcgactggcg gatgacggta
     1381 tacagaacaa atgcaatgat aaaagcggca gaaatggata gacagtcaat aatgagttca
     1441 gtgaatttcc agctgctttt cagcaccaga ccgtatgcag aaaaaaagat tcctgcacct
     1501 gctgcacaaa aaggaagcag cataatgaat gcttccggca gagaaaagga aaatcctttt
     1561 tctctgatcc tgaccaaaat cagaagaaat gaaatgagaa acacaggaag actgatcagg
     1621 agaatagata ttctcagata taaaacaaaa gccggatcag tcataatttt tttctcgtct
     1681 gcggactttt ccgcttttga aatttcctgt tctgattctg tgaggaacat ccacactttc
     1741 gagcattgct tccatactga tcagtctttc ataagaaata tgcttttcat ccatcttgtc
     1801 agtcagaaga cagccgtcct gaagattgca gcatcctgta attggacatt tttttctgat
     1861 cttgaaaaat tcaggaaaag aggatacaat atctcccttt ttcatgtgaa gaattcccca
     1921 ttccttgatg ccgggagaat caatgagggt gattccgtca ggcagaaaaa tcatatgaga
     1981 attggtggtt gtatgttttc cttttcctga ggaatggctg acttctccca ctttcatcat
     2041 attctctcct gaaactgcgt tgacaagact ggattttccg gcaccggaaa ttcctgcgag
     2101 aaaagtcacc ttgtccttca gcttcagtct gagttcttca atgccctctc cggtcgctgc
     2161 ggacactgtc attacattat atcccaactc cctgtagaca gatgtttttt cttcaataaa
     2221 atccgcatcg ggaagatcac atttagtaaa aataatcaat ggagtgattc ctgcaaaatg
     2281 acaggatgca aggcatctgt cgatgaatcc cggctttgtt tccggatttg caagagaggc
     2341 cagaatggct gcatagtcaa tatttgcaca gagagcctgc gcatccccgt ccctgctttt
     2401 gcggagcagt gcattttttc tttcaacccc tgacatgatt ttataatcat cacccgaatt
     2461 tgctcctgca tctgcattca caatatctcc cggtgtgaga ggatttcttt cagtggaacc
     2521 cgaaagtctg agcttgcccc gaagcactgc tcttttcaag cctgtttcag gactgtagat
     2581 ttcatagtaa gcaccgtaga ttctagtgat tacatactga cctttctcca gattcataaa
     2641 tcttcgtccg accagcccac atctgccagg aagtcgtcat aacctccgtc ataaatgctg
     2701 atcatatcat tgtcgaacac aacgagtttt gtcgccacag cacgaaggtg catttcgttg
     2761 tgagttacca taattacaga accttcaaac tgatcaatcg cctcaatcag agcatcgcag
     2821 gactgcatat ccaggtggtt cgtaggctca tccagaaaca gaagactgca gggagtgaca
     2881 agaatttttc ccagaaggac ccggcttttt tcccctccgg aaagaacctt gatttttttc
     2941 agagcactgt ccccggagaa catcagacct cctgcaatat ttcttgcttt tccttcatta
     3001 cagtccttgt ctgcactcat gatttcttct acgattgtat tgctgtcgtt gagatccagt
     3061 ttgtttgtct gtccgaaata gccttctttc agaacagggt gcttgttcac tgtccccgaa
     3121 acaggtttga attctccagc cagaatcttc agaagagtgg actttccctt cccgttcttt
     3181 ccgatgatgc agatcctgtc gccgttccct acgcttaatg aaaatttgtt tataatatat
     3241 ggctcttttc cagtataaga aaaactgatt tcttctgc
//
LOCUS       DAOQGJ010000194         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17082_length_3273_cov_3.887197, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000194 DAOQGJ010000000
VERSION     DAOQGJ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17082_length_3273_cov_3.887197"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="PK683_04240"
     CDS             complement(<1..1714)
                     /locus_tag="PK683_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004433066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNH07687.1"
                     /translation="MSAANRIKDKTILIIGGGLLQCPIIETANSLKLKTVAADMNPEA
                     PGLKIAKDRIVMSTKDIEGMVREAKRYAEHSPIHGVITAGTDASMTVSAVANALELPG
                     IRFHDAEAASNKVKMRKRLKEHSVPIPAFAEVWSIQDARDALEYLKFPLVIKPADNMG
                     ARGVIKVENRDDLHNAFKHAKKYSPSGEVILEEYMQGPELSIDALAWNGNFRITGVAD
                     RIIEREPFFIEIGHTMPSHLSKEVLQEAERVMIAGMKALGIHLGAGKGDIKVTPTGVK
                     VGEIAARLSGGFMSAYTFPLSSGINLNRAALMIALGEEPDELEPKWEKVSIERALIAS
                     PGKLISIKGVDEARDIECVNEIFMQNQPGDIISDPTNNIEKSGHVIITADTLKQAEEI
                     FEKVKNTIQLETDNYYSLNEKIINQTARQRFGKEICWVCRVCDGTDCASGVPGMGGVG
                     RMETFQDNIRALKEFTILPRYIRDAVSADTSIEFLGRKFSHPMMSAPMTGAATNMNSA
                     MDEFSYALAVLKGTKESGGLAWLGDGATPDKYKIMLDALKRNEGEGVLICKPRADEGL
                     LRERF"
     gene            2212..3147
                     /locus_tag="PK683_04245"
     CDS             2212..3147
                     /locus_tag="PK683_04245"
                     /inference="COORDINATES: protein motif:HMM:NF036760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNH07688.1"
                     /translation="MKNILFCSDPMNSKKVDPEYENEFNVCRKYNVNVGLIHYEEIMM
                     GNIQKSVKNIPHSDEAAETVYRGWMLSKENCILLYNELLKKNVKLINDPDKYLFCHYL
                     PLNYETIKEWTPKTVWADLAGGTEYDIFEVMKRIEIFNGKPVILKDYVKSQKHYWKEA
                     CYIENSKDINIVKSVIDRFIELQGENLTGGLVFREFIEFEFLNCHPKSNMPLTKEFRL
                     FFFKNRLAAEARYWDEGIYTDDVIENIGIFKDIAEQVKSNFFSMDIAKSKTGKWWIVE
                     LGDGQVTGFPGSIDTDSIYKKIIGQSELLSSEQVV"
BASE COUNT          888 a          670 c          668 g         1047 t
ORIGIN      
        1 cgaacctttc tctgagaagg ccctcatctg ctctgggctt gcagatcaga actccttccc
       61 cttcgtttct cttcagtgcg tcaagcatga ttttatattt atcaggcgta gccccgtctc
      121 cgagccaggc aagtcctcct gattctttag tccctttcag taccgccagc gcatagctga
      181 attcatccat tgcactgttc atattggttg cagctcctgt cataggagca gacatcatag
      241 gatgactgaa ttttcttccc agaaactcga ttgaggtgtc tgcagacact gcgtccctga
      301 tgtacctggg gagtatggta aactctttaa gtgcacgtat attgtcctga aatgtctcca
      361 ttctgccgac cccgcccata ccgggaactc cggaagcaca gtccgttccg tcacagaccc
      421 tgcagaccca gcagatttcc tttccgaatc tctgtcttgc agtctgatta atgatttttt
      481 cattaagaga ataataattg tccgtctcca gctgaatagt gttctttact ttttcaaaga
      541 tctcttctgc ctgtttcagt gtatctgctg taatgatgac atgtcccgat ttttctatat
      601 tgttcgtcgg atcgctgatg atgtccccag gctgattctg catgaaaatt tcattaacgc
      661 attcaatatc cctggcttcg tccacgccct tgatggaaat gagtttgccc ggagaagcga
      721 tcaaagctct ttcaatggat actttttccc atttcggttc gagctcatcc ggttcttctc
      781 caagtgcaat catcagtgct gctctgttca gattgattcc tgaagaaagc gggaatgtat
      841 atgcagacat aaaccctccg gagagacgtg ctgctatctc acctaccttc actccagtcg
      901 gagtcacttt gatatctccc tttcctgcac ccagatgaat tcctagggcc ttcattcctg
      961 cgatcattac cctttctgct tcctgaagaa cttctttgga aagatgagag ggcattgtat
     1021 ggccgatctc aatgaagaaa ggttcccttt ctatgatcct gtctgcgaca cctgtaattc
     1081 tgaaatttcc gttccaggca agtgcatcaa tggagagttc cggtccctgc atatattctt
     1141 cgagtatgac ttcaccggag ggagaatatt tctttgcatg tttgaatgca ttgtgcagat
     1201 catctctgtt ttccactttg atgactcctc ttgctcccat attgtctgca ggtttgatca
     1261 ccaggggaaa cttcagatat tccagtgcat ctcttgcatc ctgaattgac cagacttctg
     1321 caaaggcggg gattggaaca gaatgctcct tcagtctttt tctcatcttc actttgtttg
     1381 atgcagcttc tgcatcatga aaacgtatcc caggaagttc gagtgcattt gcaactgcag
     1441 aaactgtcat gcttgcatct gtgccggcag tgattacacc gtgaatagga gagtgttccg
     1501 catatctttt tgcttccctg accattccct caatgtcttt tgtgctcatc actattctgt
     1561 cttttgcaat tttcagtcca ggtgcttctg gattcatatc agcggctact gtcttcagtt
     1621 ttaatgaatt tgctgtctct atgatcgggc actgcagaag cccgcctccg ataatgagaa
     1681 ttgtcttgtc tttaatccgg ttcgccgcgc tcaaaatttc ctgccttctt cttcagattt
     1741 ttttttggcc ggaatttgaa aatagaaaaa tcctcagttt tttcatattt acagttatca
     1801 gattgtcggt gatttcaatt attcccttga catttgttca agcattgtga tatgatagac
     1861 tgaagatgtt ttatgaggtt tgaatatgaa acagatgctc tttcttgtcg ctctagtact
     1921 tgcaatagga acccaatccc tgacggcaaa atgccaatac aattctgatt gaaaaaatcg
     1981 gatggaaaac ggatcatcgt atgtgtcaaa gggaacagtt tcagtgatcg ggataaggca
     2041 agaactgcct gcacggaaat caaaggctct tcctgcggca gcagcgaatg ctgcaatgaa
     2101 tcaggcaggc accatcactc actttccggg ttccagtctg ctctgcaaga ggctgtgtaa
     2161 aagcactgtc cgtttttaca cagtctttgg cggaatattt ttggagattt aatgaaaaat
     2221 atactgtttt gttctgatcc gatgaactca aaaaaagtgg atcctgaata tgaaaatgaa
     2281 tttaatgtat gcaggaaata taatgtaaat gtcggattga tacattatga agagattatg
     2341 atgggaaata ttcaaaaatc agttaaaaat attcctcatt ctgatgaagc tgcagaaaca
     2401 gtatatcgcg ggtggatgct tagtaaagag aattgtattc tattgtataa tgaacttctg
     2461 aaaaaaaatg taaaattgat aaatgatccg gataaatatt tattttgtca ttatttacct
     2521 cttaattatg aaacaataaa ggaatggacc ccgaaaacgg tatgggcaga tttggcgggg
     2581 ggcactgaat atgatatttt tgaagttatg aaaagaatag aaatattcaa cggcaagccg
     2641 gtaattttga aggattatgt caagtcgcaa aagcattact ggaaagaagc ctgttatatt
     2701 gaaaattcaa aagatatcaa tattgtaaaa tccgtaatag acagatttat tgaacttcag
     2761 ggtgaaaatc tgacaggcgg attggttttt agagaattta ttgaatttga gtttctaaac
     2821 tgtcatccga aaagcaatat gcctttgacg aaagagtttc ggctgttctt ttttaagaac
     2881 aggctggctg cagaagcacg ttactgggat gaaggaattt atactgatga tgtaatagaa
     2941 aatatcggta tttttaaaga tatagccgaa caagtgaaaa gcaatttttt cagtatggat
     3001 attgcaaaat caaagacagg gaaatggtgg attgttgaac ttggcgacgg gcaggtgacc
     3061 ggttttcccg gaagtataga cacagattca atatacaaaa aaataatcgg acagtctgaa
     3121 ttactctcct ctgaacaggt tgtttaaagt gaatatttat cttcccatat gcatactttt
     3181 ttcaggtttt cttttttatc tttactggag acttgcagtc gttcatcttc ctgttcttca
     3241 tttttccaga aataaattta atgataaaat aat
//
LOCUS       DAOQGJ010000195         3268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17134_length_3268_cov_3.309057, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000195 DAOQGJ010000000
VERSION     DAOQGJ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17134_length_3268_cov_3.309057"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PK683_04250"
     CDS             complement(<1..1189)
                     /locus_tag="PK683_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07689.1"
                     /translation="MKKNILIILILIMAASSNLQSEEITPLQISILSPMQLYPERSSV
                     LGMRFNVFYGKNENVYGLDAGLVNHTRKNSAGIAAGLINLSENLVGVQAGVFNGTENL
                     YGIQAGIGNYTKNPYGIQAGAVNIADKAYLPMFGILANWADRPYLGQYSVGFNQASDA
                     RLQISGILNNARKGGVLQISAGVNYGDSILQLSGLWNESKDATFQLAGGINTADYSAS
                     CQTAILYNRTEKAKLQIAVFGNSSGKSGLQLAVFGNYSPKSAVQISGIVNIGENNPVQ
                     TAGLFNFSSCAAECNRIFQISPIANYSSKGFFQFGTVNTAGEMEIQSGAVNLSDKVKG
                     TQIGLVNRSLETEGTMFGLINESHDLTGYQIGLINIAKNGKYPIMLFYNSNYEKREIR
                     KGSG"
     gene            1298..2689
                     /locus_tag="PK683_04255"
     CDS             1298..2689
                     /locus_tag="PK683_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH07690.1"
                     /translation="MNIYGLLLLVSLTVRCTSTMYPRHFEGASQYECKAEYCVLKEGK
                     IPFGKKLHSVSRNKFNDEWYHRIQDDREEIYIPESMLSDTPVRIPVRVRSVEGADFYA
                     DAGLKEKIGHLRFQESAEILEEIWNSEKNIKKYKIICKNITGYINGGDFDLPGKKGFF
                     KVSVLSGLNLREKPDQNSRILTVVPYSYEGEVLDRDRRIMEIQRKKGYWFFTEYEKKR
                     GWMFSGHVFLSERKDFSSDLREPLAAFHQTFLNPGIIRPEVYKKYPVISDEVKYDQLL
                     KYRNSESSAEISGFSFLTQTPVIRNECNEYETRLILQNRAENIQYFLNPENLSESVLK
                     ERGRFILTEYTGCWCCCGNFGENIYIVMKNKLQKYSFLKEAFAVRSECSLHPEKIRYS
                     KNKDSLYLLFRYAECTMDENTGTLKFLKFKDTLFISLKTDGENVQMKRVFHKEIPNEF
                     KSDWDSSEKLKGE"
     gene            2715..>3268
                     /locus_tag="PK683_04260"
     CDS             2715..>3268
                     /locus_tag="PK683_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07691.1"
                     /translation="MIKVFLLILTFVLHCNVYPQVSDSEMKKIEKKWGIEEDGEETVP
                     VRSKLSSAVPAPLLPFQAPVQEFKINPLQETRVKGKAGIEIIIPANSFVLPENFRERN
                     TLRAELIEIINDLDFMASGIDLVWYGRNGVPHIFESGGMFRLNVLFQNRTVPLKKGKK
                     LTVQIPFYSYGRKMKMYRLNNAGE"
BASE COUNT          956 a          637 c          722 g          953 t
ORIGIN      
        1 gaccggagcc ctttctgatt tctctttttt cataattgga attataaaaa agcatgatag
       61 gatattttcc atttttggca atattgatga gaccgatctg gtatccggtc agatcatgac
      121 tttcattgat tagaccgaac atagtacctt ctgtttccag ggaacggttg accaggccga
      181 tctgcgttcc tttcacttta tcggacagat tgactgctcc tgactgtatt tccatttctc
      241 cggctgtatt gactgtacca aactgaaaga atcctttcga ggaataattt gcaattggac
      301 tgatctgaaa aattctattg cattccgctg cgcaggaact gaaattgaaa agacctgcgg
      361 tctgaacagg attgttctct cctatattca caattcctga aatctgcaca gctgatttcg
      421 gggaatagtt tccgaataca gccagctgca gtccgctttt cccgctgctg ttcccgaata
      481 ctgcaatctg aagctttgct ttttctgtgc ggttgtaaag aatagcagtc tggcaagatg
      541 ctgaataatc tgccgtattg attccgcctg ccagctgaaa agttgcatcc ttcgattcgt
      601 tccagaggcc tgaaagctgc agtattgagt ctccgtaatt cacgcctgct gatatctgca
      661 gaactcctcc cttccgggcg ttgttcagta tgcctgaaat ctgcagacgt gcatccgatg
      721 cctggttgaa gccgacgctg tactgtccca gatacggacg gtctgcccag ttcgccagaa
      781 tcccgaacat tggcagatag gccttgtctg caatgttcac agcacctgcc tgtattccat
      841 aaggattttt agtataattt cctatacctg cctgaatccc gtaaagattc tctgttccgt
      901 tgaatactcc tgcctgcaca ccgaccagat tttcggaaag attgatcagc cccgctgcga
      961 ttcctgcaga attttttctt gtatgattga caagtcctgc atcgaggccg tatacatttt
     1021 catttttccc gtagaaaaca ttgaacctca ttccaagcac tgagctcctt tccggataga
     1081 gctgcatcgg ggaaagaatg ctgatctgaa gcggagtgat ttcctccgat tgcaaattgg
     1141 aagaagcagc cataataaga atcaaaatga ttaggatatt ttttttcaac gtttggttct
     1201 ccatcagaaa ttctgatcag taagcattgt tctcgggata actttcggga caatgagttt
     1261 tttgtttacg gaaaatttat ctgacgaaaa tgaagatatg aatatttatg gattacttct
     1321 tttggtttca ctgactgtca ggtgcaccag cacaatgtac ccgcgtcatt ttgagggagc
     1381 atcacagtac gaatgcaagg ccgagtactg tgttctgaaa gaagggaaaa ttccttttgg
     1441 aaaaaaactg catagtgttt ccagaaataa attcaatgat gaatggtatc acagaattca
     1501 ggatgatagg gaagaaatct atattcctga atctatgctt tcggatactc ctgtacggat
     1561 tcctgtaagg gtacgcagtg tagaaggggc agatttttat gcagatgccg gactgaaaga
     1621 aaaaatagga catctgagat ttcaggaatc agctgaaata ctggaagaaa tatggaattc
     1681 agaaaagaat ataaaaaaat ataaaataat ttgcaaaaat ataacagggt atataaacgg
     1741 aggagacttt gatcttcctg gaaaaaaagg attttttaag gtgtccgtcc tttcgggtct
     1801 gaatctccgt gaaaaacctg atcagaattc cagaattctt acagttgttc cttattccta
     1861 tgaaggtgag gttctggaca gagacagaag gataatggaa attcagagaa agaagggtta
     1921 ctggtttttt actgaatatg aaaaaaagcg gggctggatg ttctccggtc atgtttttct
     1981 ttcagaacga aaagatttca gctctgatct tcgagaacct ctggcagctt ttcaccagac
     2041 ctttctgaac cctggaatca tcagaccgga agtatataaa aaatatcctg taatatccga
     2101 tgaagtcaaa tatgatcagc ttctgaaata cagaaattcg gaatcttctg ctgagatttc
     2161 cggtttttca ttcctgactc agacgccggt tatcaggaat gaatgcaatg aatatgagac
     2221 cagactgatt cttcagaaca gggctgagaa tattcagtat tttctaaatc cggaaaacct
     2281 ctctgaatct gttctcaaag agagaggacg ttttattctg actgagtata caggatgctg
     2341 gtgctgctgc ggaaattttg gagagaatat atatattgta atgaaaaata aattacagaa
     2401 atactccttt ctgaaggaag cattcgctgt cagatctgaa tgcagtctgc atcctgaaaa
     2461 gatacgttat tcgaagaata aggattcttt gtatctgctt ttccgttatg cggaatgcac
     2521 gatggatgaa aatacaggaa cgctgaaatt tctgaaattc aaggatactc tttttatctc
     2581 tctaaaaacc gatggagaga atgttcagat gaaaagagtt tttcataaag aaatccccaa
     2641 tgagttcaag tctgactggg attcttccga gaaactgaaa ggagaatagg ggtggttctt
     2701 cgaaggagtt tacaatgata aaggtttttc ttctgatcct gacttttgtt ctgcactgca
     2761 atgtttatcc gcaggtgtcc gattcggaaa tgaaaaagat tgaaaagaaa tggggaattg
     2821 aggaggacgg ggaagaaact gttcctgtca ggtcgaagct gagttccgct gttccggcac
     2881 ctctgctccc gttccaggcc cctgtgcagg aatttaaaat caatcctctg caggagacgc
     2941 gggttaaggg aaaggcaggc atcgagatta tcatacccgc aaattcattt gttctccccg
     3001 aaaacttccg tgaaagaaat actctcagag cggagctgat tgaaatcatc aatgatctgg
     3061 attttatggc atccggaatc gaccttgtct ggtacggcag aaacggtgta cctcatattt
     3121 tcgaatcagg aggaatgttc cggctgaatg ttctgtttca gaacaggacc gtgccgctga
     3181 aaaaagggaa aaagcttaca gtgcagattc ctttctattc atatggcaga aaaatgaaaa
     3241 tgtatagatt aaataatgca ggagaatg
//
LOCUS       DAOQGJ010000196         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17212_length_3255_cov_3.276250, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000196 DAOQGJ010000000
VERSION     DAOQGJ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17212_length_3255_cov_3.276250"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..1558)
                     /locus_tag="PK683_04265"
     CDS             complement(281..1558)
                     /locus_tag="PK683_04265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH07692.1"
                     /translation="MTFEDLKIIEPVCRALAEEGYTNPTPIQEKSIPSALTGTDILGL
                     AETGTGKTAAFTIPVLQTLYNRRKAGEGYQGIQALILAPTRELAIQIGESINTYGRHT
                     SLKYAVIFGGVPQRNQERVLKNKIDIVVATPGRLLDLADQRIVKLHNINMLILDEADR
                     MLSMGFIDDVRRIVKKLPSRRQNFFYSATMPDEIQRLADEILKNPFRAETGQLSAPNR
                     NIEQEVYQVDKINKHDLLVHLLQDETLSKVLVFSKTKHTANKVSDELNRKSIQSAAIH
                     GNKTQAARRKALESFKKGKLRVLVATDIASRGIDIDEVTHVINYELPNEAEAYIHRIG
                     RTGRAGLSGKAISFCAREEKTFLRSIEKLSRQQIQVNLNHPYNYGTASTKPKENGHSA
                     SSNSNGYNSYREQDSRRGGQKFKPRSRNQQRHR"
     gene            complement(1609..>3255)
                     /locus_tag="PK683_04270"
     CDS             complement(1609..>3255)
                     /locus_tag="PK683_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07693.1"
                     /translation="KINQPKSETTCLKYILAGAFLFHISVLWGNFTWDDSILFLEAKT
                     YSCGTDFSRVFTKPFYFNVNYFRPIISALYYFESCIYGTVPFFYKLLNLILFTAASGL
                     VFQFFNALTEPLKDRKRWIPIGAASVWIIHPIQSELISQATNRFDLLMIIFSVLILLN
                     SLKNGRRQYVLSFSLSFISILTKETAVIFPVLILFFALHRQNSFQVSKAVFLHPALSA
                     AGILAALSLRPLLMGYVWKSGGMPIEPGDFISHILLTARTAVTDLRILLLFKLNGPIH
                     YSELPVLMNPFSISELVLFSASAAFSIFIAFRKPEIGTLLACFWVSLLPVLQIYPIEL
                     SGNSITAERYLALPFFLLLSAGVKWAIYNENILINIFFRTGFIIFLSAEAVVGQYMHA
                     VWNSSESLWNHSLWTAPKSKIPYLSLALIYAAENRHEKAAETADMAVSLYPPTSRRDS
                     WDTSILYFNRANSLMRLKRFQAAEASYWKSIELSENLHMQNNRAYINLSICLLVLGRR
                     EESHYILKKLASENPGNREIQNAVRFIYENEEKSVGEFRN"
BASE COUNT          880 a          635 c          718 g         1022 t
ORIGIN      
        1 ggatataaaa aaaactgaac gtattgggtt ttttattctt cgactgagct cagtgcaacg
       61 ctcggtggcc tacggtgcaa actgtattga tgcatcattt cgtacggtaa atttgtaatt
      121 tcaatattca tgaaattttc attaaaattg gatggagctg ttttggggtt ttaattgtat
      181 aaagcgggtt aagggcaatt cacagagtcg gtcattttat gccggtcact gttctgaaat
      241 ttcagacatc tccgtgtcag ccctcgtttt cagagtttaa ttatctgtgt ctttgctgat
      301 ttctgctcct tggcttgaat ttctgtccgc ctcgtctgct gtcctgttca cggtaactat
      361 tgtaaccgtt tgaattggaa gatgcagaat gaccgttttc cttcggcttg gttgaagcag
      421 ttccataatt gtaaggatgg ttcagattta cctgaatctg ctgtctggaa agtttttcta
      481 tggatcttaa gaaagttttt tcttctcttg cacagaatga aattgccttt cctgaaagtc
      541 cagctcttcc tgttctgccg attctgtgaa tgtaggcttc tgcttcgttc gggagttcat
      601 agttgattac gtgagtcact tcatcaatat caattcctct tgatgcaatg tcagtagcta
      661 caagcactct gagttttcct ttcttgaaac tttcaagagc ctttcttctg gccgcctgag
      721 ttttgtttcc atgaattgct gccgactgta ttgattttct gttcagttcg tcagatacct
      781 tgttcgcagt atgttttgtc tttgagaaaa caagaacctt tgaaagagtt tcatcctgaa
      841 gcagatgcac aagcagatca tgtttattga ttttgtccac ctgataaact tcctgttcga
      901 tatttctgtt tggcgcagaa agctggccag tttcggcacg gaaaggattt ttcagaattt
      961 catcagcaag tctctgaatt tcatcaggca ttgttgcgga ataaaagaag ttttgtcttc
     1021 tggaaggaag tttctttacg attcttctca cgtcgtcaat gaatcccatg ctcagcattc
     1081 tgtctgcttc atctaatata agcatattta tattatgaag ttttacaatt ctctggtcag
     1141 cgagatccag aagtcttcct ggagttgcta caacgatgtc tattttgttt ttcaggactc
     1201 tttcctgatt tctctgcgga acaccgccga atattactgc atatttcagt gatgtatgtc
     1261 tgccgtatgt gttgatgctt tctccgatct gtattgcaag ttctcttgtc ggagccagaa
     1321 tgagagcctg aattccctga tatccttcac ctgcttttct gcggttgtaa agggtctgca
     1381 ggacgggaat tgtaaatgct gcagtttttc ctgttccagt ttcagccaga ccaagtatgt
     1441 ctgtgcctgt cagcgcagat ggaattgatt tttcctgaat tggagtaggg ttagtgtaac
     1501 cttcttctgc gagtgctcgg cagacaggtt caatgatttt caaatcttca aatgtcatta
     1561 gtcattattt tccggcctct taggagggaa agaactcttt ttttaatctc agttcctgaa
     1621 ttctccgaca gatttttcct cgttttcgta aataaagcgc acagcattct gtatttccct
     1681 gttacccgga ttttctgagg ccagtttttt cagaatgtaa tgagattctt cccttcttcc
     1741 taagacaagc aggcatatgc tcagattgat atatgcccta ttgttctgca tatgcaggtt
     1801 ttccgacagt tcaattgatt tccagtagga agcttcagcg gcctggaatc ttttcagccg
     1861 catcagtgaa tttgcacggt tgaaatagag gatcgaagta tcccaggaat cccttctgga
     1921 tgtcggcgga tacaatgaga ctgccatatc tgcggtttct gccgccttct catgtcggtt
     1981 ttcggcagca tagataagtg ccagactcag atacggaatt tttgattttg gcgcagtcca
     2041 tagagaatga ttccagagac tttcagagct gttccatact gcgtgcatat actgtccaac
     2101 gactgcttct gcactcagaa agatgatgaa tcccgtccga aaaaaaatat ttattaatat
     2161 attttcatta tatattgccc attttactcc agcagagagc agaagaaaaa aaggaagagc
     2221 aaggtatcgt tcagcagtaa ttgaattgcc ggaaagttca attggataaa tctgcagaac
     2281 aggcagaagt gaaacccaga agcaggcaag cagagtcccg atttccggct ttcggaaggc
     2341 aatgaatatg ctgaatgctg cagatgcact gaataagact agttccgaaa ttgaaaaggg
     2401 gttcatcagt acaggaagtt ctgaatagtg aatgggtccg ttcagtttga aaagaagcag
     2461 aattctgaga tcagtgactg cagttctggc agtcagcagg atatgagaaa taaaatctcc
     2521 cggttcaata ggcataccgc ctgatttcca gacatagccc ataagcagag gtctgagaga
     2581 cagagccgca agaatgcctg cagctgaaag ggcaggatgc agaaagacag cttttgagac
     2641 ctgaaaagaa ttttgtctgt gaagtgcaaa aaaaagaatc agcacaggaa atatgactgc
     2701 tgtttccttg gtcaggatcg aaataaagga gagagaaaaa ctcaaaacgt actgccttcg
     2761 tccgttcttc agtgaattca gaaggataag cacggaaaat atgatcatca gaagatcaaa
     2821 tctgtttgtg gcctgactga tcagttcgga ctgaatggga tgaatgatcc atacagatgc
     2881 ggcaccgata ggaatccatc tttttctgtc tttgagaggt tctgtcagtg cattgaagaa
     2941 ctgaaatacc agtcctgatg cagcagtaaa cagtatcaga ttcagaagtt tgtagaagaa
     3001 agggaccgtt ccgtaaatgc aggattcaaa ataatataag gcacttataa taggtctgaa
     3061 gtaattcaca ttgaaataaa agggttttgt aaagactctg gaaaagtcgg ttccgcacga
     3121 atatgtcttt gcctccagaa aaagaattga atcatcccag gtaaaatttc cccagaggac
     3181 agaaatgtga aaaagaaatg ctcccgcgag aatgtatttc agacaggttg tttccgattt
     3241 tggctgattg atctt
//
LOCUS       DAOQGJ010000197         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17258_length_3251_cov_4.010325, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000197 DAOQGJ010000000
VERSION     DAOQGJ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17258_length_3251_cov_4.010325"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1094)
                     /locus_tag="PK683_04275"
     CDS             complement(168..1094)
                     /locus_tag="PK683_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07694.1"
                     /translation="MELTRKVMEVSSEQKFYADPSKVTGISKIVFWPRSHRPFQGEFY
                     HDLFNRRVFSCELYSERLERNQLNEMYEQNLLTYEELGLEDFSAFQRKYALKVPEGMV
                     FFSEEDKADQNHLRIRAVNLTGYLNLPLEYLVVLSPGWYPVWKQVRKVYISRDSQFLY
                     FTEDANRFTVYEIRKDKAVSRIVLYEISQLQYNSLSPIDQDYRKDIRLVRGALDTAKL
                     TELPIKEQLSGIRILAFKNMHYVFDMSDKLKIKENETVYIEQRYLSEPSSADYCEVEA
                     VPLRYGYYPLLAVTVPVDFVLLPLWLYFGLSR"
     gene            complement(1152..1451)
                     /locus_tag="PK683_04280"
     CDS             complement(1152..1451)
                     /locus_tag="PK683_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07695.1"
                     /translation="MFRIKSLTVSAVLFFHFIGCSSSLIFPLTRVTNRWILIEDGKPD
                     YYLDTYAMRNKTKLNYLLFSLNAVTVPLDLALIPALIIGAPIYMTIAVLLFPVKI"
     gene            complement(1534..2223)
                     /locus_tag="PK683_04285"
     CDS             complement(1534..2223)
                     /locus_tag="PK683_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07696.1"
                     /translation="MKNLLILMYFALSLSLVHWIGFRNYCIDCWGLLNHLYQKNNVLL
                     HSEEKNHCSVDSIKEIRLHNTPDRFGVIDIEIHFKARSSFEWSECSENVFFRIHRMDP
                     EEKSGIIENTGAIQSWSKEYAYTRLKNEGRLNFLLEETDSEKEAFIDVSFVDSDKKTH
                     WKLFKISDQKHKEKYKHIQYKIRLEPSEINKTYFYLYPFLVTAEILLLPLEYAVFYTY
                     WFLSGGLSSVY"
     gene            complement(2518..3093)
                     /locus_tag="PK683_04290"
     CDS             complement(2518..3093)
                     /locus_tag="PK683_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07697.1"
                     /translation="MITFALALFLFIDSLLLSSPGNDKFWGSSKKEIGAGLQNAERLY
                     KIPAPIRKLFLKKNIKVFTIDLDSDGKRDYIISETDAFRTCFADSRFHLKSCEKIGQG
                     DGFSYYYFVKLDSDPMLKLFDFSGDEDYSDFKLYSFSPKTWKLQFLSEVHPYIEVEDK
                     EHRGIYWGYPWDITGIRLMKNQRGIHFFGGL"
BASE COUNT          966 a          722 c          558 g         1005 t
ORIGIN      
        1 ccgcaagtta aaagcttaat ccaaaattgc attgtgtttc tttcacagta cgactttgtc
       61 aagcccaccg atttatcagc tgccttattt aagcttacct ttccagtaat ctgcatccag
      121 tgcctcccat atcttaaaaa aacttttccc acatccaaat ctttccatca acggcttaat
      181 ccaaaataca gccacagggg cagaagaaca aagtcgacag gaacagtaac agcgagaagc
      241 ggataatacc catatcgaag agggactgct tccacttcgc agtaatcagc agatgatggt
      301 tctgacagat agcgctgctc aatataaact gtttcatttt ccttaatttt taatttatcg
      361 ctcatatcaa atacatagtg catatttttg aaggcaagga tacggattcc tgacagctgt
      421 tccttgattg gaagctctgt cagtttcgct gtatccaaag cacctcttac cagtcggata
      481 tctttcctgt aatcttggtc aatgggagac agagagttat actgcaattg cgagatttca
      541 tacagaacaa tcctgctgac tgctttatcc tttcgtattt catatacagt gaatctattg
      601 gcatcttcgg taaaataaag aaactggctg tcccgagaga tatagacctt tctgacttgc
      661 ttccagactg gataccatcc tggggaaagc acaacaagat actccagagg caggttcaga
      721 taaccggtca ggttgaccgc gcgaattcgc aaatgatttt gatccgcttt gtcctcttca
      781 gaaaaaaaca ccataccttc cggaaccttc agtgcatact ttctctgaaa tgcagaaaag
      841 tcctcaagtc caagctcttc atatgtcagc agattctgct cgtacatttc attcagctga
      901 ttcctttcca gtctttcaga ataaagctca caggaaaata cccgacggtt gaacagatca
      961 tgatagaatt ctccctgaaa cggcctgtga ctgcgaggcc aaaatacaat cttactgatg
     1021 cctgtaacct tagatggatc tgcataaaac ttctgttcgc tgctcacttc catcaccttt
     1081 ctggtgagtt ccatattttg gcagctgact ataaaaatga aggatgcaat tttcagaatt
     1141 ttcataaaat actatatttt gacagggaaa agcaatacag caattgtcat ataaattggc
     1201 gccccaatga taagggcagg aataagagcg agatccaaag gaacagttac tgcattcaaa
     1261 gagaaaagca gataattcag ctttgtttta tttctcattg catatgtatc taaataataa
     1321 tctggttttc cgtcttctat cagtatccac ctattcgtca ctctggtaag cgggaatata
     1381 aggctggaag aacatccaat aaaatggaaa aacaaaactg cacttactgt caatgatttt
     1441 attctaaaca tatcctctct tctgcctgtc aagcagatca agattaccat atgatcctgc
     1501 gtttgtattg taaaaaacgg ccatttcact gaatcaatac accgaagaca gaccgccgga
     1561 caaaaaccag taagtataga aaactgcata ttccagaggc aataacagaa tttctgccgt
     1621 caccagaaac ggataaagat aaaaataggt tttatttatt tcagacggct ccagacggat
     1681 tttgtattgg atatgtttat atttttcctt atgtttttgg tcggaaattt taaacagctt
     1741 ccagtgagtt tttttatctg aatcgacaaa agaaacatca atgaaagctt ccttttctga
     1801 atcagtctcc tccagcagaa aattcagccg tccttcattc ttcagccttg tatatgcata
     1861 ttctttgctc caggactgaa ttgcgccggt gttttcgatg atacctgatt tttcctccgg
     1921 atccattcga tgaatcctga aaaatacatt ctcggaacat tcagaccatt caaaactgct
     1981 tcttgccttg aaatgaattt caatatcaat tacaccgaac ctgtcaggag tattgtgcag
     2041 tctgatttcc tttatggaat ccacggaaca gtgattcttt tcctcactgt gcaataaaac
     2101 attatttttc tgatacagat gattcagcag tccccagcag tctatgcagt aatttcgaaa
     2161 tccaatccag tgaaccaatg aaagggacag agcaaaatac atcagaatca gcagattttt
     2221 cactgtaatg ttccgtgcat ttctgtaaat gagcatatac taagcatatt tatgtaatac
     2281 ataaattatt caactgggat attctgagca gcagaacagg aatgcatctg cattctttga
     2341 ctcgtccgag tctgtctgca tttattcctc tgactgagca tctgctttaa tgtgatcggt
     2401 ttgaatcttt ttttaagatc tgtataactt ccattttcct ccccttgagt gggagttcct
     2461 ctgaacagaa tctttgcagc acagcctgtt tctcctgtca tatcgtcttt gaatttttca
     2521 cagaccccca aaaaaatgaa ttcctcgctg attcttcatc agtctgattc ctgtgatatc
     2581 ccagggatac ccccaatata ttcccctgtg ttctttatct tccacctcaa tgtaaggatg
     2641 cacttcagac agaaactgca gtttccaggt tttcggactg aaagaataca gtttaaagtc
     2701 gctgtagtct tcatcgcctg aaaaatcaaa aagcttcagc attggatcgc tgtccagttt
     2761 cacaaaatag taataggaaa aaccgtcacc ctgtcctatt ttctcacagg attttaaatg
     2821 aaatctgctg tccgcaaaac atgttcggaa tgcatctgtt tcagaaataa tatagtctct
     2881 ttttccgtcg gaatcaagat caatagtaaa aacttttata ttctttttta agaacagttt
     2941 cctgatcggt gcaggaatct tataaagtct ttctgcattc tgcagtcctg ccccgatctc
     3001 ttttttagaa cttccccaga acttatcatt tcccggtgaa gacaggagca gactgtcaat
     3061 gaataaaaac aaagccaatg caaaagttat catattcctg cagtacatct tgacccatgt
     3121 ttttctctca tagaattctc cgcactgata aaccagtttt ccaatctgac tttttactcc
     3181 taagctcctg cttttttcag cagttttatg gtttcagatt tgccgctaaa aacagctcca
     3241 ttcagaggag t
//
LOCUS       DAOQGJ010000198         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17397_length_3233_cov_3.290434, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000198 DAOQGJ010000000
VERSION     DAOQGJ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17397_length_3233_cov_3.290434"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..2464
                     /locus_tag="PK683_04295"
     CDS             386..2464
                     /locus_tag="PK683_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07698.1"
                     /translation="MKKKEIVSLTVLLSLLAYCSSDEKGTKAGKQKRDAALAIMLSQT
                     PYDPDQAAIDAVDADIVNPGPLNMNVKSVTASSSAVSLGKNCRLSASIQSDYDAKNVL
                     IRFLFVNKSQIDAGITSDLKQFEVLDRIPYIKKGTVSYDFNLSIPDAGTSHSGSYTIY
                     TILDPENKRDSSFQKSGMLSGVNRSGAAVVTVDSSSSGTRDLYISSLKARDSVIILKP
                     NQTYVKSRIGISLEAFSRNKDAENADVEFFFADTNGTKLTSLGNLLVYNSSSGTGSQY
                     FRFSTMKADQKANADLILIPSSAAALANMQNYVQTNGLNSLKVYAEINPTGAVSEVNS
                     PGGATSNSASVSVTAAVDTGIKYVPLVGTIPGDGKAISASLNTDPLKEYSAGYTTGTL
                     GDKSLFAVDMDIKAYGKILKNTTVIGHGEAKSNIYMFDIISRTLFQAYAHAELIPIRM
                     SSSFVDAEVKAMNLSGDLAVVYKKYETGAKSYYWYTTKYKDKKIEKRIFVYGVPVKLT
                     GVIAGILLSKNKNQLSECTINSCRPEEFNEGFSEINYKDYSVSQIHVRNNFEINVNPR
                     ILLRGFAQAKPDTGVTMGLEGQLTILDIFPSAKGTAAVRMVNNAYQVEIEFKETLDVF
                     MEYLYGKLEMFVEIPYPKISFWDASIEYYRKYWTLVSWNSLGDFKDRFLDESQFIRIH
                     LYTGEINFTY"
     gene            2603..>3233
                     /locus_tag="PK683_04300"
     CDS             2603..>3233
                     /locus_tag="PK683_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07699.1"
                     /translation="MNKKKIIPILFLSFVGILVWSILWSIWTFFTSANFEWTLNDRKM
                     YRFQSEAFQITSSEQFRLKGTGLETKINAVLNFRVLSVEKERVKLAFQFSPFEMEIGG
                     KRMPALEELNSVMFLMEWDRDGNIMRMIFPSEVAKKDEALITQNLLLFQSIIIGSRRN
                     WMTKEDDTLGRFTAKYNYKDGKILKSKLRYLKIFDEDGDRNTPDDEDLKS"
BASE COUNT         1037 a          647 c          673 g          876 t
ORIGIN      
        1 taaatctgtt tattcttttc ggacgctgag tttgaaaaaa agaattctca ataatttcaa
       61 aatctgaaat ttcatgattc agttcaggga aaaccctgaa tccattcttc tgcatccgat
      121 ctttttcttt tattattggc ttcctgagcg aaagcggaag caaatcattt ttgcgtctcc
      181 ttctcatttt gagaatattt ttttacaaag aaccctgtat ttcacaattg tattttaaca
      241 tactatttta ctaaagttca ttatgaattt aaaatcggcg atccggaaga tattcaggga
      301 aaaccctgaa aggtttactt tttataaaaa gactccgatt taaaaagagt attttccgtc
      361 gtatgaattc aatgatgagg tatatatgaa aaaaaaagaa attgtttcct tgacagttct
      421 tctgagtctg cttgcctact gcagcagtga cgaaaaagga accaaagcgg gaaaacagaa
      481 aagggatgct gcactggcaa taatgctctc acagactcct tacgatcccg atcaggctgc
      541 aattgatgca gttgatgcag acattgtaaa tcccggacct ctgaatatga atgtaaaatc
      601 tgtcactgcc tcctcatcgg cagtgagtct gggaaaaaac tgcagactct ctgcttcaat
      661 acagagtgat tatgatgcaa agaatgtgct catccgtttc cttttcgtaa acaaatcgca
      721 gattgatgca ggcattacgt ccgacctgaa acagtttgag gttcttgaca gaattccata
      781 tataaagaag ggaacagtca gctacgactt caacctttcc attcctgatg caggcacatc
      841 ccacagcgga tcgtatacga tatatacaat attggatccg gaaaacaaaa gagacagttc
      901 atttcagaaa agcggtatgc tttccggcgt gaacagatcc ggagcggctg tcgtcacagt
      961 ggattcatcc tcatcaggaa caagagatct gtacatcagc agtctgaaag ctagagactc
     1021 agtcatcata ctgaaaccga atcagacata tgtaaaaagc aggattggaa tatctcttga
     1081 ggcattctcg agaaacaagg atgctgaaaa tgcagatgtg gaattcttct ttgcggatac
     1141 gaacggaaca aaactcacgt cactcggaaa ccttctggta tacaattctt cctccggaac
     1201 aggatctcag tatttcaggt tcagcacaat gaaggcagat cagaaggcaa atgccgatct
     1261 gattctaatc ccctcctctg cagcagcact cgccaatatg cagaattatg ttcagacaaa
     1321 cggcctgaat tctctgaaag tctatgcaga aatcaatccg acaggagctg tatctgaagt
     1381 caattctccc ggcggagcca cttcaaattc tgcttcagtt tcagtcaccg ctgcagtgga
     1441 tacaggaatc aagtatgtac cgcttgtcgg aacgattccg ggagacggaa aagcaatcag
     1501 tgcttcattg aatactgatc ctctgaagga atattctgca ggatatacca ctggaactct
     1561 cggagacaag tctctttttg cagtggatat ggatatcaag gcatacggaa aaatactgaa
     1621 gaatacaaca gtaatagggc atggagaagc aaagagcaat atatatatgt ttgatattat
     1681 cagcagaact ctgtttcagg catacgctca tgcagaactg atcccgatca gaatgtcctc
     1741 ctcttttgtg gacgcggaag tcaaggcgat gaatctttca ggagatcttg cagtcgttta
     1801 caaaaaatat gagacagggg caaaatccta ttactggtat acaactaaat acaaggacaa
     1861 gaagatagag aaaagaatat tcgtatacgg tgtccctgta aaacttacgg gagtcattgc
     1921 agggatactg ctgtccaaaa ataaaaatca gctttctgaa tgcaccatca attcctgcag
     1981 acctgaagaa tttaatgaag gtttttctga aatcaattac aaggactaca gtgtatctca
     2041 aattcatgtt agaaacaatt ttgaaatcaa tgtgaatccc agaatcctcc tccggggatt
     2101 tgcacaggca aagccggata caggggtcac tatgggactg gaaggccagc tgaccattct
     2161 ggatattttc ccttcggcaa aaggaaccgc agcggtaaga atggtgaaca atgcatatca
     2221 ggtggaaatc gaattcaagg aaacactgga tgtatttatg gaatatcttt atggaaaact
     2281 ggaaatgttt gtggaaattc cgtatcctaa aatcagtttc tgggacgctt ccatcgaata
     2341 ctaccgcaaa tattggacac ttgtcagctg gaattctctg ggagatttca aggacaggtt
     2401 tctggatgaa agccagttta tcagaatcca tctctatacc ggagaaatta actttactta
     2461 ctgaatgcat tcggcaaaaa ggatatgatg tcaagaacct catatccttt tatccgctca
     2521 gagacttcaa aacagttgag ggtctctgcc cttctcattc cggtttccgt tcagccgtct
     2581 ctaagccctc gaaaatactg caatgaataa aaagaagatc attccaatac tgtttctgtc
     2641 tttcgtagga atccttgtct ggagcattct ctggagcata tggacatttt ttacgagtgc
     2701 aaattttgag tggacactga atgacaggaa aatgtacaga tttcagtccg aagcatttca
     2761 gattacttcc tccgaacagt tccgcctgaa aggaaccgga ttggaaacaa aaatcaatgc
     2821 tgtgctgaac ttcagagttc tttcagtaga aaaggaaaga gtaaagcttg cctttcaatt
     2881 ctctcccttt gaaatggaga tcggcggaaa aagaatgcct gcactggagg aactcaattc
     2941 agtaatgttt cttatggagt gggacaggga cgggaatatt atgagaatga tatttccatc
     3001 agaggtagcc aaaaaagatg aggctctcat aacccagaat cttcttcttt ttcagtccat
     3061 cattatcgga agcagaagaa actggatgac caaggaagat gacacccttg gacgttttac
     3121 cgcaaaatat aattataaag acgggaagat tctaaagtca aagttgagat atctcaaaat
     3181 ctttgatgaa gacggtgaca gaaatactcc cgatgatgaa gatctcaagt caa
//
LOCUS       DAOQGJ010000199         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17418_length_3231_cov_3.170025, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000199 DAOQGJ010000000
VERSION     DAOQGJ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17418_length_3231_cov_3.170025"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK683_04305"
     CDS             complement(<1..311)
                     /locus_tag="PK683_04305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH07700.1"
                     /translation="MNRVEYSIIIPTFNEKGNPSMISEKIIRELDGKFDFEIIFIDDD
                     SPDLTWQECEKLAESDSRIRVLRRAGRRGLNSAVLEGMSYGKGNFFAVICISFIILVE
                     L"
     gene            complement(314..691)
                     /locus_tag="PK683_04310"
     CDS             complement(314..691)
                     /locus_tag="PK683_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07701.1"
                     /translation="MKYKIIKIKHSTLRWKPLKRLQRNVQFHLLWMLAVLMIAGAASK
                     RLYSEYAKSSSGELYAGECLSVCSKIEACLRESFPSEFSLYGKTVQSGCIVKCPKHIA
                     KVRNCVTENDCGNMIPCYIKENR"
     gene            complement(672..2183)
                     /locus_tag="PK683_04315"
     CDS             complement(672..2183)
                     /locus_tag="PK683_04315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNH07702.1"
                     /translation="MHIQTIRESFRNGNYFLPFLNGVPNPYKPPLIFWIGMAAESIVP
                     ESLFMERLPSVLFAAGTAVLVFRISGIFGGQRLASFTAGFIYILTVGVFKFSRLVMME
                     QAMAFFLSLCTYFILMYIVHNRKKYIVLAGLCAGAGFLLKGPVLTVYAGTVLFVWGVL
                     HFFRFRFAPFQWKGRKYLHRMLSGVLLFFIPAAFLPVLYYIVLTLYADNGIGIVKFFL
                     FIENIGKFSNENQPELAIFLGAVFYLFPWSIAVSVLAFRLLFQRIRKPRALLGIILTV
                     SSAFILLIHILPNRKDAYYSIPALPLLISGVFIKYGNYIYSFKNLWKMNAYFSLLILF
                     LTVSGAFFLQTIDDFGISPFFSVAVLFMCGSVLFLKISYVYRNILVSFLILAFFQFYL
                     LRKTYLPLIPEDLNLGGKSVCILSENSWNLFDAGIFLPGSRLYYSAPSLPENCPPDSP
                     SLLALGTHRTPDSRFEKKRSWKIWKPHSLVRDFLNRDRSRIFEEVSYYEIQNH"
     gene            2527..3144
                     /locus_tag="PK683_04320"
     CDS             2527..3144
                     /locus_tag="PK683_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH07703.1"
                     /translation="MHSKTKRSEAKENTRCILMQTSLELFASNGFNGTSMEMIARKAG
                     VSKGLAYTYFASKNEILIEIIKTFTDKKRTILKSIIGESTTPFEKIKKLIESAVDTDA
                     ENEAGRDMHRLLYMIKLQPGFSEMLQSLSSEMKPQILEDMEMIDSLLKKIGVKNPAKE
                     REYIKTVITGIMILYLSCPKDPPFPYPYEQLKKDLIDRYRPENWK"
BASE COUNT         1126 a          652 c          637 g          816 t
ORIGIN      
        1 tatagctcga ccaaaataat gaaacttata caaatcacag caaaaaaatt tcctttacca
       61 tacgacattc cttcgagaac ggcagaattc agtccccttc tcccagctct tctgagaact
      121 ctgattctgg aatccgattc tgcaagcttt tcgcattcct gccaggtcag atcaggggaa
      181 tcatcatcta taaaaatgat ttcaaaatca aattttccat ccagttccct gatgatcttt
      241 tcagaaatca tagaaggatt tcccttctca ttgaatgtcg gtataattat agaatattca
      301 accctgttca tattcagcga ttctctttta tgtagcaggg aatcatattc ccgcagtcat
      361 tttctgttac acagtttctc acttttgcaa tatgcttagg acacttgacg atgcagcctg
      421 actgaactgt ctttccgtac agagaaaact ctgaaggaaa actctccctg agacaggctt
      481 caattttact gcatacagaa agacattccc cggcatacag ctctccggat gatgattttg
      541 catattcgga ataaagcctt ttcgatgctg ctcctgcgat catcaggact gcaagcatcc
      601 aaagaaggtg aaactgaaca tttctctgaa gtctcttcaa aggcttccag cggagtgtac
      661 tgtgttttat tttaatgatt ttgtatttca taataagaaa cctcctcaaa gattctgctt
      721 ctgtcgcggt tcagaaaatc ccgaacaaga gaatgcggct tccagatttt ccaggatcgt
      781 tttttttcga acctgctgtc cggagttctg tgagttccca atgcaagcaa ggaaggcgaa
      841 tcgggagggc agttttcagg cagactggga gcactgtagt agagcctgga tcctggcaga
      901 aagattcctg catcaaacag attccatgaa ttttcagaaa gtatgcagac tgatttccct
      961 cccagattca agtcttccgg aatcagagga agatatgtct tcctgagaag ataaaactgg
     1021 aaaaaggcaa gaatcagaaa tgaaacgaga atattccgat acacatagga aatcttaaga
     1081 aaaagaacgg agccgcacat aaatagaaca gccacggaga aaaaaggaga aataccgaaa
     1141 tcatcaattg tctgaagaaa aaaagcaccg ctgacagtca aaaaaagaat gagcaagctg
     1201 aaataagcat tcatcttcca gaggttttta aaactgtata tataatttcc atattttata
     1261 aatacacccg aaatgaggag aggcaatgca gggattgaat aataagcatc cttcctgttc
     1321 ggaagaatat gaataagcag tataaatgcc gatgaaacag tgaggataat tcccagaagt
     1381 gctctcggct tccgtattct ctgaaacagt aaacggaaag caagaacaga aacggcaata
     1441 ctccaaggaa acagataaaa aactgcgcct agaaaaatgg caagttcggg ctggttttca
     1501 tttgaaaact ttccaatgtt ctcaataaat aagaagaatt tgactatgcc gatgccgtta
     1561 tccgcataaa gtgtaagcac aatgtaatag agaaccggca ggaatgcggc agggatgaaa
     1621 aacagcagta ccccggagag catcctgtgc agatactttc tgcccttcca ttgaaaaggt
     1681 gcaaatctga atctgaaaaa atgcagaaca ccccatacaa aaaggactgt tcctgcatat
     1741 actgtcagaa cggggccttt cagaagaaaa cctgctccgg cacagagacc agccaataca
     1801 atgtattttt ttctgttatg aacaatatac atcagtataa aataagtgca aagggaaaga
     1861 aaaaacgcca tagcctgttc catcattacc agtcttgaaa acttgaatac tcctactgta
     1921 agtatatata tgaatcctgc agtaaatgat gccagtctct gacctccgaa aatacctgag
     1981 attctgaaca caagcactgc tgtccctgct gcaaaaagaa ctgaaggaag tctttccata
     2041 aacaggctct ccggcacaat gctttctgcc gccattccga tccagaaaat caaaggaggt
     2101 ttgtacggat ttggaacacc gttcagaaag ggaagaaaat aatttccgtt tctgaagctt
     2161 tcacgaattg tctgaatatg catactttca tctccctgag tcagaattgc gtacgaggaa
     2221 attccgggaa tgaggagaat aaggcaggac ataacgagaa gaaaagaaag aaacagattg
     2281 ctttcagtca atgctctttt cgattgcata tgaaccatat atatttgacc gaacggtcag
     2341 tcaaatatat tttcaatcaa ctgttcaccg aattctcttt tttatacagc ttttaaaaaa
     2401 aacagttttc aaaaatcgta taaactctgt acaataggaa aactgaacac tgctcagaaa
     2461 accaataaac atacagaaag ttttttaaaa ggactgaaca gtcccatact cggaaaggtt
     2521 aagaaaatgc acagtaaaac caaaagatcg gaggcaaagg aaaatactag atgcattctg
     2581 atgcaaactt ccctggaact ttttgcctcc aacggattta acggcacctc aatggaaatg
     2641 attgccagaa aggcaggagt atccaaagga ctcgcctaca catacttcgc aagtaaaaat
     2701 gaaatcctta tcgagatcat aaagactttt actgacaaaa aaagaacgat tcttaaatcc
     2761 attattggcg aaagtacaac tccgtttgaa aagataaaaa agcttatcga aagtgcagtg
     2821 gatactgatg cagaaaatga agcaggcagg gatatgcaca gacttctcta catgatcaag
     2881 cttcagccgg ggttttcgga aatgcttcag tccctgtctt ctgaaatgaa gcctcagatc
     2941 ctggaagata tggaaatgat tgacagtctg ctgaaaaaga tcggtgtaaa gaatccagca
     3001 aaagaacggg agtatatcaa gacagtgatt actggaataa tgatattata tctcagctgt
     3061 cccaaagatc ctccttttcc atatccgtat gagcagctga aaaaagatct gattgacagg
     3121 tacaggccgg aaaactggaa atgaaactga ctgaagcatt gaatgaacct tgtaagggag
     3181 aagaaaatgt attatgattc actcaaacga aagagaaaag aggagaagaa a
//
LOCUS       DAOQGJ010000200         3217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17536_length_3217_cov_3.509804, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000200 DAOQGJ010000000
VERSION     DAOQGJ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17536_length_3217_cov_3.509804"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PK683_04325"
     CDS             <1..839
                     /locus_tag="PK683_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07704.1"
                     /translation="IFLFPFSMGAVVVYFADTRFKKSFLFQAFAPWGVSAVSLFISML
                     VGWEGTICLIMALPVYLLMSSLGGLAMGAFLRMTSSKSLQMVSFAFLLITPFISGKIE
                     QKFDLPQEIRTVDTEITVQAQPETLWSRITVIPKITEPQESFFYYMGFPKPVEATLSY
                     EGVGGIREAVFERGLLFLERITVWEENRRLSFSILADPRSTPLTALDPHVTVGGYFFD
                     TLLGEYEIEPKSEKETVLKLRSKFRVSTRFNFYSSLWADFLMADIQKNILRVIKSRAE
                     KG"
     gene            871..1941
                     /gene="lepB"
                     /locus_tag="PK683_04330"
     CDS             871..1941
                     /gene="lepB"
                     /locus_tag="PK683_04330"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNH07705.1"
                     /translation="METAENTKKKSDTKEPEKKETFAGSTVSLFLIIFFVFAFKSSIL
                     DANNIPSGSMIPTLKIGDFLFVNKMRYSLRIPFFSETEIFRYDDPKRGDIVTFNPPDD
                     AIQEDIYSIDSAGNVTRPKKVQIPILSDIFAKRFVKRVVGMPGDEIRISQKRFKAANG
                     EFVDYTWIEYREKGSDEFKTYSPAPVQNNKELSDVDNLRAIGKSLFTEEKRDFKHYVL
                     EGYHSASYSFIRRNCPENICKIPEGHYLVMGDNRDDSSDSRFWGFVRREDILGKALIL
                     YFSINWRDFTCMFKSIPEDNSLESVNLQRYSKDELEKQCTAAELNNEFHSESLPEWLF
                     RTLRYRIWRMNVRWERIGRILQ"
     gene            1942..2871
                     /locus_tag="PK683_04335"
     CDS             1942..2871
                     /locus_tag="PK683_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNH07706.1"
                     /translation="MTTGIVLSEFSKGHDTGSHPEQILRYDWIKKKISEKSYFSEVRK
                     VKPVRASKEDICRVHSDEYYESLVQRNNGIVQRLDPDTVFSPGTFEAAEYSAGGGIAL
                     ADEILSGRLKNGFCVSRPPGHHAEKGFAMGFCIFNNAAVTAKYLKHKGAGKVFILDWD
                     VHHGNGTQHIFYDDDSVFFLSLHQFPYYPGTGSSDEKGSGKGLDCTLNIPLQRGSTDS
                     DYKKAFETSVLKAMDDFHPDFIIISAGFDAHAKDPLGGMNLSTSAFEFFTSAVREKAE
                     ELCQGRIISHLEGGYDLDALADSAEAHFAALKG"
     gene            2879..>3217
                     /locus_tag="PK683_04340"
     CDS             2879..>3217
                     /locus_tag="PK683_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07707.1"
                     /translation="MKIIYFIILMYLIGCSSSQDMESYSEGSTGKAAPEFLPYMEKWR
                     KEFRWRQSMTEAEFKRFQKMNYAPEEIENIKKNVLGKNPFATFKNYFIIQKFAGFEID
                     RKNYAGAVKIW"
BASE COUNT          989 a          587 c          712 g          929 t
ORIGIN      
        1 tcatttttct tttcccgttt tctatgggag cagttgttgt ctattttgcc gatacaagat
       61 ttaaaaaaag ctttcttttt caggcatttg ctccctgggg agtttctgcc gtcagtcttt
      121 ttatttccat gcttgtcgga tgggaaggaa caatctgtct gattatggct cttcctgtat
      181 atctgctgat gtcttctcta ggcggactcg caatgggagc ctttctgcgg atgaccagca
      241 gcaaatcatt acagatggta tcttttgcat ttcttttaat tacgccgttc atttctggga
      301 aaatagagca gaaatttgat cttcctcagg aaatcagaac agtggacacg gaaattaccg
      361 ttcaggcaca gccggaaacc ctatggagca ggattaccgt gattccgaaa atcacggagc
      421 ctcaggaaag ttttttttat tatatgggct ttccaaaacc tgtagaggca accttaagtt
      481 atgagggagt aggcggcata agagaggcag tttttgaaag agggcttctt tttctggaaa
      541 gaatcactgt ctgggaagaa aaccgccgtc tcagcttcag tattctggct gatccaagat
      601 ccactcctct tactgcactg gatcctcacg taactgtagg cggatatttt tttgatacgc
      661 tccttggcga atatgaaatt gaacctaagt ctgaaaaaga aactgtcctg aagctcagga
      721 gtaaattcag ggtttctaca agattcaatt tctattcatc tctatgggca gattttctga
      781 tggcagacat tcagaagaac atacttcgag tcatcaagtc aagagccgaa aaaggctgaa
      841 atcataatta taaattaaac aaggcagcat atggaaacag cagaaaacac aaagaagaaa
      901 tcagatacaa aggagcctga gaagaaagag acatttgcag ggtcaactgt ttcattattt
      961 ctcatcatat ttttcgtatt tgcattcaag tcatccattc ttgatgcaaa caacattcct
     1021 tcaggttcta tgatccctac attaaaaatt ggagattttc tttttgtaaa taagatgagg
     1081 tattctctca gaattccttt tttttcagag actgaaattt tccgatacga tgacccaaag
     1141 cgcggagaca ttgtaacttt taatccgcct gacgacgcaa ttcaggaaga tatctactca
     1201 attgacagtg ccggcaatgt gaccagacca aagaaagtgc agattccgat tctgagcgac
     1261 atatttgcaa agagatttgt gaaaagagta gtcggaatgc ccggggatga aattcggatc
     1321 agccagaaaa gattcaaggc agcaaacgga gagtttgtag attatacttg gattgaatac
     1381 agagaaaaag gatcagatga gttcaagaca tacagtcctg cccctgtaca gaacaataaa
     1441 gagctttctg atgttgacaa tcttcgtgca attggaaagt cgcttttcac tgaagaaaaa
     1501 agagatttta agcattacgt tctggagggt tatcacagtg cttcctattc attcattaga
     1561 aggaactgtc cggaaaacat atgcaaaatt ccggaagggc attatcttgt tatgggagac
     1621 aacagagacg attccagtga ttcacgattc tggggatttg tcagaagaga ggatatatta
     1681 ggaaaagctt taatccttta tttctcgatc aactggaggg attttacatg catgttcaag
     1741 agtatacccg aagacaacag tcttgaatca gtcaatctgc agagatactc caaggatgaa
     1801 ctggaaaaac agtgtacagc cgcagaactg aacaacgagt ttcattcaga aagtctgcct
     1861 gaatggcttt tcagaacgtt aaggtacaga atctggagaa tgaatgtacg atgggaacgg
     1921 ataggaagga tactgcagta aatgacaaca ggcatagtgc tttctgaatt ttccaaaggt
     1981 catgatacag gaagtcatcc tgaacagatc ctcagatatg actggataaa gaaaaaaatt
     2041 tccgagaagt cctatttttc agaagtaagg aaagtcaagc ctgtcagagc atcaaaagag
     2101 gatatctgca gggttcattc ggatgagtac tatgaaagcc tggtgcaaag aaacaatggt
     2161 attgtgcaga ggcttgatcc tgatacagtt ttttcacccg gcacttttga agctgctgag
     2221 tattctgcag gcggcgggat agcacttgcg gatgaaattt tgagcggccg tctgaagaat
     2281 ggattctgtg tttcgagacc tccaggtcat catgctgaaa aaggttttgc gatggggttc
     2341 tgcatcttca acaatgccgc tgtcactgca aaatatctga aacataaagg tgcaggaaaa
     2401 gtatttatcc ttgactggga tgttcatcat gggaacggca ctcagcatat tttttatgat
     2461 gacgattctg ttttttttct ctcacttcat cagtttcctt attatcctgg gacaggaagt
     2521 tcggatgaaa aaggatctgg aaaaggattg gactgtactc tgaatattcc tcttcaaaga
     2581 ggcagtactg attctgacta taaaaaagca tttgaaactt ctgttctgaa ggccatggat
     2641 gatttccatc ctgattttat cattatatct gctggatttg atgctcacgc aaaagaccct
     2701 cttgggggaa tgaatctttc cacgtctgcc ttcgagtttt ttacttcagc tgtaagagaa
     2761 aaagcagaag aactgtgcca aggaagaatt atatctcatt tggaaggcgg ttatgatctg
     2821 gatgctcttg ctgattctgc tgaggctcac tttgctgcat taaaaggatg attctgtaat
     2881 gaagataatt tatttcatta ttctaatgta tcttatcggc tgctcatctt cccaggatat
     2941 ggagtcttat tctgaaggca gcacgggaaa agcggctcct gaatttcttc cctatatgga
     3001 aaaatggaga aaggaattcc gctggagaca gtctatgaca gaagcagaat ttaaaagatt
     3061 ccagaagatg aattacgccc ctgaagaaat agaaaatata aaaaaaaatg ttctcgggaa
     3121 aaatcctttt gcaacattca aaaattattt tatcatacag aagtttgccg gatttgaaat
     3181 tgacagaaag aattatgcag gtgcagtgaa gatctgg
//
LOCUS       DAOQGJ010000201         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17548_length_3216_cov_4.057893, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000201 DAOQGJ010000000
VERSION     DAOQGJ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17548_length_3216_cov_4.057893"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /gene="folP"
                     /locus_tag="PK683_04345"
     CDS             <1..825
                     /gene="folP"
                     /locus_tag="PK683_04345"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNH07708.1"
                     /translation="CGFLEIMEIFGILNITTDSFSDGGLYLEENSAREKAISLFGEGA
                     DIIDIGAQSSNINAKPINYNEEIERIQSVLPYLQDRKIRTSIDTFRPEVIEFAIESNV
                     DYINNIRAFSDSTSLEILKKSAHTLPDLILMYSHNSGEKASEKSTLHPDKVMDDIYRF
                     LDVHRKRLISAGVPETKLIFDPGMGMFLGPDPELSFKVLKNISEFKKEFGRILVSVSR
                     KSFIGAVLNMVPPEKREIGSLALEIYLAESGVDFIRTHNPKILREALTVRSRLISG"
     gene            complement(837..2450)
                     /locus_tag="PK683_04350"
     CDS             complement(837..2450)
                     /locus_tag="PK683_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07709.1"
                     /translation="MKLQFKLTLVIGGVALLSVLPLSYFSLKQSQKIILEKTNDVCRN
                     LAQNISNIAREELFLDNTYDGTQSVISKLKESEITGLINAYIINVYGRFVVDMHNRRT
                     GQQISPEELKFFESVSSPSSDSYEMQNSEILRFSFPVYLDESHPLRIGFAVIEFDQKI
                     LYAPVANIRNFIIYLSIGIFILVGMVSVLTSVAITKPISRLTEGAKIIGDGDYQHRIF
                     LPVRDEIGSLATAFNDMTERIQKSDKLKNDYMDAYSRFVPQEFLKFLKKESILEIKLG
                     DQIQTEMTVLFSDIRSFTNLSETMTPKENFDFLNAYLERVGPVIRKNKGFIDKYIGDA
                     IMALFPYTPEDSIQACVEMHKVIRVYNEKRDKKGYKPIKIGVGIHTGNLMLGTIGENQ
                     RMDGTVIADAVNLASRIEGLTKMYGASTIISERTFFGLSDPDKYHYRQLDNVQVKGKK
                     EVVSIFEILDGMEENLLKLYIDTKRDFEKGILSYQIQQFHEALECFNSVLSVNPGDIA
                     SQKYTDRCSKAIEFGISETWDGVERLSTK"
     gene            complement(2611..>3216)
                     /locus_tag="PK683_04355"
     CDS             complement(2611..>3216)
                     /locus_tag="PK683_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07710.1"
                     /translation="NIFQSILKKDPKNGTARYYLSETVNISAKAEIDILWNKAEKLQK
                     TKDYRGARVNYKKILDLNPEYLPAKDGIENSLKNESGDIRMDAKQKDSAGFPFPAIRR
                     YKEALAILPSNREAQNELSAIRQREMRKLEDYFKTGITLYNKRDYSKSFDIFSDLLLL
                     VPEDKKIQEYQRLSLIKKNAMSRLSSCSSSTGEKCSLLKKK"
BASE COUNT          880 a          640 c          617 g         1079 t
ORIGIN      
        1 tgcggctttc tggaaataat ggaaattttt ggaatattaa atataactac agattccttt
       61 tctgacgggg gactgtacct tgaggagaat tcagcgagag aaaaagcaat ttctctcttt
      121 ggggagggag ctgatattat tgatatcggt gctcagtctt ccaatattaa tgcaaagcca
      181 ataaattata atgaagaaat tgaaagaata cagtccgttc ttccatatct gcaggataga
      241 aaaatcagga cctctattga tacatttcgc cctgaagtga ttgaattcgc cattgagtca
      301 aatgttgact atatcaataa tatcagagca ttcagtgaca gcacttcatt ggagatttta
      361 aaaaaatctg ctcatacact tcctgatctg attctgatgt attcacacaa ctcgggcgag
      421 aaggcatctg agaaatcaac cttgcacccc gataaagtta tggatgatat ataccggttc
      481 cttgatgttc acagaaaaag acttatttct gcaggtgttc ctgaaactaa gctgatcttt
      541 gatcctggaa tgggaatgtt tcttggccct gatcctgaat tgagttttaa ggttctgaag
      601 aacataagtg aatttaaaaa agagtttgga aggatattgg tttctgtttc aaggaagtct
      661 tttataggag cggttttgaa catggttcct ccagagaaaa gagagattgg ctcacttgct
      721 ttggagatct atcttgcgga atccggtgtt gattttattc ggactcacaa ccctaaaatc
      781 ctcagagaag ccttgactgt cagaagccgt ttaatctcag gataaataga atataattat
      841 ttagtactca gtctctctac tccgtcccag gtttcactga ttccaaattc aattgcttta
      901 ctgcatcggt cggtatattt ttgcgaggca atatctccag gatttacact tagaaccgaa
      961 ttgaagcatt ccagcgcttc gtgaaactgc tgtatctgat aggaaaggat tcccttttca
     1021 aaatctctct tcgtgtcaat atacagtttc agaagatttt cttccattcc gtccagaatt
     1081 tcgaaaatgg aaacaacttc cttttttcct tttacctgca cgttatccag ctgtctgtaa
     1141 tgatacttgt ccggatcaga tagaccgaaa aatgttcttt cactgataat tgtgcttgca
     1201 ccgtacattt tggtcagtcc ttcaatccgc gaagccagat ttactgcatc tgctatcaca
     1261 gtgccgtcca ttctctgatt ttccccaatc gttcccagca tcaggtttcc tgtatgaatc
     1321 cctactccga tttttatcgg tttatatcct tttttatctc ttttctcatt atatactcgg
     1381 atgaccttat gcatttcgac acaggcttga atggaatctt ccggtgtgta tgggaacagg
     1441 gccataattg catctccaat gtatttatca atgaaccctt tgttttttct gattactggt
     1501 cccactcttt ccagataggc attcagaaag tcaaagtttt ctttcggcgt cattgtctca
     1561 gaaaggtttg taaatgaccg aatatctgaa aacagaaccg tcatctcagt ctgtatctga
     1621 tctcccagtt ttatttccag gatggattct tttttgagaa acttcagaaa ttcctgtggc
     1681 acaaatcgac tgtaagcatc catataatcg tttttcagtt tgtcagattt ttgaatcctt
     1741 tcagtcatat cgttgaatgc agttgccagt gaaccgattt catctctcac gggcagaaat
     1801 attctgtgct ggtagtcgcc gtcaccgata atttttgctc cttcagtaag tcggcttata
     1861 ggtttggtga ttgccactga agtgagcacc gaaaccattc ctacaagaat gaatattcct
     1921 atggaaagat atatgataaa atttctaata ttggctacag gcgcataaag gattttctgg
     1981 tcaaattcta ttacagcaaa tccaatccgg agcggatgcg attcatccag atacacaggg
     2041 aagctgaatc tcagtatttc agaattctgc atttcataac tgtcggaaga gggactgctg
     2101 acggattcaa aaaacttcag ttcttccgga gaaatctgct gacccgtacg tctgttatgc
     2161 atatctacta cgaatctccc atatacattt ataatatagg catttatcag tcctgtgatt
     2221 tcggactcct tcagtttaga gatgacagac tgtgttccgt catatgtatt gtccaggaaa
     2281 agttcttctc gtgcgatatt tgatatgttc tgagcaaggt ttctgcacac atcattcgtt
     2341 ttttcaagga tgattttctg actctgcttc agagaaaaat aggaaagcgg cagaacactt
     2401 aaaagtgcaa cacctcctat gactagagta agcttaaact ggagtttcat tgggtatcct
     2461 caagatatga aagtttgtcc tgaatgccga aaaggaacgg aagaaacctt ttttaatgac
     2521 agtacctgcc tgaaacggtt ctgaaattct tatccgattc agtggattaa tgaaattttt
     2581 tatattatag ctatgcatac atatccggga tcatttcttt tttaataatg agcatttttc
     2641 tcccgtactg ctgctgcagc tggaaagtct tgacatagca ttctttttta tcagtgacag
     2701 tctctgatat tcctgaattt ttttatcctc tggaacgagc aggagcagat cggaaaaaat
     2761 atcaaatgat ttagagtagt ctcttttatt gtaaagtgta atcccggttt tgaaataatc
     2821 ctccagtttt ctcatttccc tctgccgtat tgcggaaagt tcattctgtg cttctctgtt
     2881 tgagggaaga atcgccagtg cttccttgta acgtcggatg gctgggaagg gaaaaccggc
     2941 agagtccttc tgttttgcat ccattcgaat atctcccgat tcatttttga gtgaattttc
     3001 tattccatcc tttgcaggaa gatactcggg attcagatca agtatttttt tataattaac
     3061 tcttgctcct ctgtaatctt tagtcttctg aagtttttct gccttgttcc ataagatgtc
     3121 aatttccgcc tttgctgaaa tattgacagt ctccgagagg tagtaccttg cagttccatt
     3181 tttaggatcc tttttcagaa tggactgaaa aatatt
//
LOCUS       DAOQGJ010000202         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17615_length_3209_cov_2.854153, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000202 DAOQGJ010000000
VERSION     DAOQGJ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17615_length_3209_cov_2.854153"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1077
                     /locus_tag="PK683_04360"
     CDS             292..1077
                     /locus_tag="PK683_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07711.1"
                     /translation="MLNGKWKILIECIPYMLGMLGMKYVISEPLGYPGMIDFSDVALV
                     LTGGIFLIGFMLAGTMADYKESEKIPGDLASIFETFEESIHQGVNANKTPIEKKIYMT
                     ELRAAIDAVMKWLLKKLSQEEMYSALSRLGTYVCKFEKEGVNGGLCGKMLGDIHNVRK
                     LVTRVGVISRTGFLATGYALLEVLIACIIAVLMIAKFKNLIAEMIIVAFVTLIYVYMY
                     KLIKDVDDPFEYDPDGETGVSEIPLFPMEEYIERLEKRMSDGN"
     gene            1067..2461
                     /gene="sthA"
                     /locus_tag="PK683_04365"
     CDS             1067..2461
                     /gene="sthA"
                     /locus_tag="PK683_04365"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNH07712.1"
                     /translation="METEKFDVIVIGTGPGGEGASIKAAKEGKKVAVIEKWDRVGGGC
                     THWGTIPSKSLRQAVQQLVEVKANPLFERTNVQITFPELLKTAENVISKQVKMRQDFY
                     TRNGIDIIKGKARFRDEHTIEVVQPDKTISAVCAADYFVIAAGSRPFRPTDIDFNSPN
                     IFDSDTILNLNRTPSSVTVYGAGVIGCEYASIFRNLGIKVNLINTRDKLLSFLDDEII
                     DALAYHLREQGILIRHNEIYSKVETVNDGVIMHMESGKQIKTDILLFANGRTGNSEGL
                     GLEKLNIKVNGRSQLDVNENYQTSVGHIYAVGDIVGHPSLASAAYDQGRFASTHIIDG
                     MCEHRLVENIPTGIYTSPEISSIGKTEKELTAEKVPYEVGHSLFKSLARAQITGRTVG
                     MLKILFHRQTLEILGIHCFGSEASEIIHIGQAIMSQKPPANTLMYFINTTFNYPTMAE
                     AYRVAALNGINRVF"
     gene            2468..>3209
                     /locus_tag="PK683_04370"
     CDS             2468..>3209
                     /locus_tag="PK683_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNH07713.1"
                     /translation="MTKRRKWLILVLLFLSSSLVAYILFLINKSKIEAAVLEKIQALS
                     GLNLKISERKLIFFPEPGIVLSQVQAENSGFSSRKIFQADAEEISFHVTWKSLLGKEI
                     GIKDAAVKGMRFHYFLEKPEKDKKEKKEQSENPFQEMKKFSMSAFSIERSSIIVYKDD
                     KIIEDFTVQEFSIDNSIIQGLEIFLDIKYLDGRAKGKVIFRNDSFTEDFDKWKFSADL
                     DLTSFRYKFLKSYYSFFHRADFDRSELTG"
BASE COUNT          996 a          583 c          784 g          846 t
ORIGIN      
        1 ggaatcacaa agaacgattg caaaaggaat atgataggga ggattttgcg aagtatattt
       61 ccagtcattc tagaaccgat cgacctccga taaagttagt tcatttttgg attttgaaaa
      121 gctgagatag agaaaggaga atcaaaaccg acagcaaaac aattgatatt tacagtgaaa
      181 tcgcgaataa aattaaaatt gaagtgtaaa aataaactgg actttgtcag cactgcattg
      241 agcattattc cggtgcctag ggatcggctg gtttagcgga ggattaaaaa tatgttgaat
      301 gggaaatgga agattctgat cgagtgcatt ccttatatgc tggggatgct gggaatgaag
      361 tatgtaattt ctgagcccct tgggtatcct ggaatgattg atttttccga tgtggcgctt
      421 gttcttaccg gcggtatttt cctgatcggt tttatgctgg cgggaacaat ggcagattac
      481 aaggagagcg aaaagattcc cggggatctg gccagcattt ttgaaacatt tgaagaaagc
      541 attcatcagg gagtcaatgc aaacaagact ccgattgaaa agaagattta tatgacggaa
      601 ctgagggctg ccatcgatgc tgtgatgaaa tggctcttga aaaagctctc tcaggaagaa
      661 atgtattctg ccctcagcag actgggaacc tatgtctgca agtttgagaa agaaggcgtg
      721 aacggcggac tctgcggcaa gatgctggga gacattcaca atgtcagaaa actggttaca
      781 agggtgggtg tgatttcgcg gacaggattt ctggccaccg gatacgctct tttggaagtg
      841 ctgattgcct gtatcattgc agtgctgatg attgccaaat tcaagaatct gattgcggaa
      901 atgattatcg tagcatttgt cactctgatt tatgtatata tgtacaaact gatcaaggat
      961 gtggatgatc cgtttgagta tgatcctgac ggtgagactg gggtttccga aattcctctt
     1021 tttcctatgg aagagtatat agaaagactc gaaaagagga tgtctgatgg aaactgagaa
     1081 gtttgatgtc atagtaatcg gaacaggccc tgggggagaa ggggcttcca tcaaggctgc
     1141 aaaggaagga aagaaagttg cagtcattga aaaatgggac agggtaggcg gcggatgcac
     1201 tcactgggga acaattccca gcaagtctct gcgtcaggcg gttcagcagc tggtagaagt
     1261 caaggcaaac cctcttttcg aaaggaccaa cgttcagatt acttttcccg agcttctgaa
     1321 aacggcagaa aatgtcattt ccaagcaggt gaaaatgcgc caggattttt ataccaggaa
     1381 cggtatagac atcatcaagg gaaaggccag gttcagagat gagcatacaa ttgaagtggt
     1441 tcagccggac aagacgatct cggcagtctg tgcggcagat tactttgtaa ttgcagcagg
     1501 atcaaggccc ttccgtccga cagacattga ttttaacagt ccgaatattt ttgacagcga
     1561 tacaatactg aatctcaaca ggactccctc ttctgttaca gtttacggtg cgggagtcat
     1621 cgggtgtgag tatgcctcca ttttccgaaa tctcggaatc aaggtcaacc tcatcaatac
     1681 aagggacaaa cttctttcat tcctggatga tgaaatcatt gatgcacttg cctatcatct
     1741 gagagaacag ggaattctga tacgtcataa tgaaatttac agtaaagtgg aaactgtgaa
     1801 tgacggtgta attatgcata tggagtccgg caagcagatc aaaacggata tacttctctt
     1861 tgccaacggg agaaccggga attcagaagg actcggtctg gaaaaactga acatcaaagt
     1921 gaatggaaga tcccagctgg atgtaaatga aaactaccag accagtgtag gtcatattta
     1981 tgcagtgggc gatatagtgg ggcatccgag cctggcaagt gccgcgtatg atcagggaag
     2041 gtttgcatcc actcatatta tagacgggat gtgtgaacac aggcttgtgg aaaatattcc
     2101 taccggaatt tacacgagtc ctgaaatcag ttccatagga aagacagaaa aagaactgac
     2161 agcagaaaag gttccttatg aggttgggca ttcacttttt aagagtctcg caagagctca
     2221 gatcacagga agaactgtag gcatgctgaa gattctgttt cacagacaga ctctggaaat
     2281 actggggatt cactgttttg gaagcgaggc ttcggagatc attcatatcg gacaggcaat
     2341 catgtcacag aagcccccgg caaacacatt gatgtacttc atcaatacaa cattcaatta
     2401 ccctacgatg gcggaagctt acagggttgc tgcattgaac ggaatcaaca gggtattctg
     2461 acggcttatg acaaaacgaa ggaaatggct gattcttgtt ctgctctttc tcagttcttc
     2521 cctcgtggcg tacattctct ttctgatcaa taagtccaaa atagaagcag ctgttctgga
     2581 aaagattcag gctctgagcg gactcaatct gaaaatctcc gaaagaaaac tgattttttt
     2641 tcctgaaccc gggattgtgc tgagccaggt tcaggcagaa aattcaggat tcagcagcag
     2701 aaaaattttt caggcagatg cagaggaaat ctcatttcat gtgacttgga aatcccttct
     2761 tggaaaagaa atcgggatca aagatgctgc agtaaaagga atgaggtttc attacttttt
     2821 ggaaaagcct gaaaaagaca aaaaggaaaa aaaagaacag agcgaaaatc cgtttcagga
     2881 aatgaagaag ttttctatgt cagcattctc catagaaagg tcttccataa tcgtatataa
     2941 agatgataaa attattgaag attttactgt tcaggaattt tccatagaca attccattat
     3001 tcaggggctt gagatatttt tggatataaa atatctggat ggacgcgcaa aaggaaaagt
     3061 cattttcaga aatgattcct tcacggaaga ttttgacaaa tggaaatttt cagcagatct
     3121 ggatcttact tccttcagat acaagtttct gaaatcatac tattcttttt ttcacagggc
     3181 tgattttgac agatcggaac tgacaggca
//
LOCUS       DAOQGJ010000203         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17656_length_3205_cov_3.128254, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000203 DAOQGJ010000000
VERSION     DAOQGJ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17656_length_3205_cov_3.128254"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PK683_04375"
     CDS             <1..343
                     /locus_tag="PK683_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07714.1"
                     /translation="KGKVLGHDSESVTIQSDGVTKTIPKSSIYKVIFSQGNSELKKFL
                     IEKKGLDSKHEDDLKDFDDNSADKKELAQKLNQLEKKIDKMEKRLGKLKDRMTKLRAK
                     IKQKEQEEKKG"
     gene            complement(350..>3205)
                     /locus_tag="PK683_04380"
     CDS             complement(350..>3205)
                     /locus_tag="PK683_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH07715.1"
                     /translation="SVSRNSVSDIDVRVDLEDKNPDKVISEIRRAVESVQNLPVQVTE
                     KPKMTERKSGSYPIYDFSLFNADNENTLQSYGVFMKEELEKLPGVARVEIFGKRDHEW
                     HISVNAEKLHSRGLDMQDIISTLKGRILNLPAGSTENENAEDIRVNGEFEQLSDMKNL
                     AVKSNEAFNQIRLSEVAEFKDTYSYPRFLAVSNGSPGLILSIVKKEKADAIHTVENVK
                     KRMTELEKTAPSGIRTFTLNDEAKRTKVRLNVVVTNAMVGFAIVFGILFLFMDFRTAV
                     ISSLSLPFALLLTFAVIPFWDVSFNIISMMGIIIALGMLVDNSIVISENIYFEMDSGT
                     SLEKASVSGAADIAMPIFGSYLTTVAAFLPMMFMSGIMGKFIWQIPLVVIISLTASLF
                     ESFFLLPARFSAFGLRSKKEESRFRKKLDSVFDSLDRGFKHLIETVLRRKYISLIIIF
                     TIIIISFISFTRMKFILFPKEDVEILLIKAEFPPSLRASDTRKKILPMEKIIQTIPKD
                     ELMSYSIKIGVQQTDPDDPLSRFGEQLAIITVFLTPESERKRKAEDIVKSIEKEIKAV
                     PELVSIFVEELVPSPPIGAAVTLSIQGKDYEKIRHLTEEMKSFMAGIKGVINVRDDYK
                     SGRNQIIIRPNEKLEALTGVSTFLIADTMRSVFDGNRIATLRKGKEKIYLRTLYHEEF
                     RKNKKNIPNIPIRTAHGPTAKLGSLSQISEKSAPEMLVHKNFERAITVYADVETASIS
                     SLQANEIIIKKFHSDFSKKYPELSLVIGGEEKDTERSMASLAKAGLFAVFGIFAILAL
                     TMNSLLKPIAILSTIPLGLIGIVLGFPLSGKAISFLAMIGIIGLSGVLVNASIVLVDC
                     IDQLQKERTDGVTYDEILAEAGRRRFRPILLTTLTTMGGLLPTAYSIGGSDPILIPMT
                     LALGWGLGFGTLGSLFYIPVLFSVSESLSQAFKKRKG"
BASE COUNT          880 a          718 c          692 g          915 t
ORIGIN      
        1 aaagggaaaa gtgctaggtc atgactccga atcggtgacg attcagtccg acggcgtcac
       61 caagacgata ccgaaatcca gcatttacaa agtcattttt tctcagggaa attcggaact
      121 gaagaaattt ctgatcgaaa aaaagggtct tgattccaag cacgaggatg atctcaagga
      181 ttttgacgac aacagtgctg ataaaaaaga actggctcaa aaactgaacc agctcgaaaa
      241 gaaaattgat aaaatggaaa aacggcttgg aaaactcaag gacaggatga ctaaactgcg
      301 ggctaaaatc aaacagaaag agcaggaaga aaagaaaggg taaaaacgat tacccttttc
      361 tttttttgaa agcctgactc agactttctg acacagagaa cagaacaggg atgtaaaaaa
      421 gactgcctaa ggttccaaat ccaagccccc agcccagagc caatgtcata ggaatcagga
      481 tcggatcgga acccccaatg ctgtaagctg tcggaagcag accgcccatt gtcgtgagcg
      541 tggtcaggag aatgggacgg aaacggcgtc ttccggcttc tgcaagaatt tcgtcatatg
      601 tcactccgtc agtcctttcc ttctgaagct gatcaataca gtcaacgagt acaatcgatg
      661 cattcactaa aacacctgag agtccaatga tcccgatcat agccagaaag ctgatggctt
      721 ttcctgagag tggaaacccc agaacgatcc cgatcagccc caaaggtatt gtggaaagga
      781 tggcaatcgg tttcagcaga ctgttcatcg tgagcgcaag aatcgcaaaa atcccgaaaa
      841 cagcaaaaag acctgctttt gcaagcgatg ccattgatct ttccgtatcc ttctcttctc
      901 ctccgattac aaggctcaat tcaggatatt ttttactgaa atcggaatga aatttcttta
      961 tgattatttc atttgcctga agggaggata tggatgctgt ttctacatct gcatatactg
     1021 tgattgccct ttcgaaattc ttgtggacca gcatttcggg agctgatttt tccgaaatct
     1081 gcgaaagact tcccagcttt gcagtcggac cgtgtgcagt tctgattgga atgttcggta
     1141 tattcttttt gttttttctg aactcttcat ggtaaagtgt tctgagataa attttctcct
     1201 tcccttttcg gagagttgca attctgtttc cgtcaaagac gcttctcatt gtgtctgcaa
     1261 tcagaaaagt gctgacccct gtcagtgcct cgagtttttc gttcggtctg atgatgatct
     1321 gatttctgcc ggatttgtag tcgtctctga cattgatgac tcctttgatt cctgccataa
     1381 agcttttcat ctcttcagtc agatggcgga ttttttcata atcctttccc tgaattgaaa
     1441 gcgtcacagc ggcaccgatc ggaggcgagg gaaccagctc ttccacaaat atgctgacca
     1501 gttccggaac tgccttgatc tccttttcaa tgctttttac gatatcctct gcctttcttt
     1561 tcctttccga ttcaggggtc aggaacaccg tgatgattgc aagctgttca ccgaatctgg
     1621 aaagcggatc atccggatct gtctgctgaa ccccgatttt aatggaatag ctcatcagtt
     1681 catccttcgg gattgtctga atgatttttt ccataggaag aattttcttt ctggtatcag
     1741 aggctctgag actgggagga aattccgcct tgatcagaag gatctccaca tcttccttgg
     1801 gaaacagaat gaatttcatc cgggtgaaac tgatgaatga aatgattata attgtaaata
     1861 ttataattaa actgatgtat tttctgcgga gaacggtctc aatgagatgc ttgaaccctc
     1921 tgtccagcga atcaaacaca gaatccagtt ttttcctgaa cctgctttcc tcttttttcg
     1981 acctgaggcc gaatgcagaa aatctggccg gaagaaggaa aaaggattcg aagaggcttg
     2041 cagtgaggga tatgattact acaagaggaa tctgccagat gaattttccc ataatcccgg
     2101 acatgaacat catcggaaga aaggccgcga ccgtagtcag atatgatccg aagatcggca
     2161 ttgcaatatc ggctgccccg gagacagatg ctttttcgag agatgtgccg ctgtccattt
     2221 caaaatagat gttctctgaa atgacaatgc tgttgtccac aagcattccc agagcaataa
     2281 tgattcccat catggaaatt atattgaaac tgacatccca gaagggaatg actgcgaatg
     2341 taagaaggag tgcaaatggg agcgaaagtg aggagatgac agccgttctg aaatccataa
     2401 acaggaaaag aatcccgaac acaattgcaa atccgaccat tgcgttggtc actacgacat
     2461 tcagcctgac tttagttctt ttggcctcat cgttcagagt aaatgttctg atcccggaag
     2521 gagctgtttt ttccagttcc gtcatcctct tctttacatt ctccactgtg tggattgcat
     2581 cagccttttc ctttttgacg atactgagaa tcagtcctgg actgccgttc gaaacagcca
     2641 gaaatcgggg gtaagaatat gtatccttga actctgccac ttctgaaagc cggatctgat
     2701 tgaatgcttc gttggatttt acagccagat ttttcatatc ggagagctgt tcaaattccc
     2761 cgttgacccg tatatcttct gcgttttcgt tttcagtgct tcctgcagga aggttcagaa
     2821 ttcttccttt cagagtggat atgatatcct gcatatcgag accccgggaa tgcagttttt
     2881 ccgcgtttac actgatatgc cattcgtgat ctctttttcc gaagatttcc actctggcaa
     2941 ctccgggcag tttttccagt tcctccttca taaaaacacc gtaactctgc agagtgtttt
     3001 cattatctgc attaaaaaga gagaaatcat atataggata acttccgctt tttctctctg
     3061 tcatcttcgg tttttcagta acctgaacag gaaggttctg aacgctctca acagcccttc
     3121 taatctctga gatcaccttg tctggatttt tgtcctccag atccaccctc acatcgatgt
     3181 cggaaacgga gtttctggat acaga
//
LOCUS       DAOQGJ010000204         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17677_length_3203_cov_2.886595, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000204 DAOQGJ010000000
VERSION     DAOQGJ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17677_length_3203_cov_2.886595"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PK683_04385"
     CDS             <1..446
                     /locus_tag="PK683_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07716.1"
                     /translation="YYLELYDSSSKWAEYLAKTHSLKTVSGSSQAWFSVDGIHLKSSI
                     RDVLEKWNEPDRKPEMEDGLQVWYYDSREVKLSMRNGFVEQIMLYGDKSPSVEGLKVG
                     SDRTAVEKFLGKNPKTQNRYLIYENKDKVGLTFEENKVERLILFE"
     gene            574..>3203
                     /locus_tag="PK683_04390"
     CDS             574..>3203
                     /locus_tag="PK683_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH07717.1"
                     /translation="MRYFIDFFLRRSLLVNILLVSIFLLALAALSRMNRNKFPEVDLG
                     TMIVTTKYPGASPKDVEQNVTRLIEDELKSVSGIDTFKSVSAENVSVVTVNIDINHPN
                     QQEVKDDIRRAVDRVSGLPSEITERPTVRDLKASEMPVLVVGVFGDAEYDELRNMAKI
                     IERDLKGIDGVSMVDKYAFRDKEFHVDLNPKKLEDLYVSLNDVLMALSNRNIRATGGS
                     LESYSTQRNILTLSQFDSIEDIRQVIIRSELGGGSVRVKDIAQVQDDFEDEKMRTIFN
                     GKQGIMLVVKKSSQADIIRVVDKIKLYLEEKQKIIPENVKLTAVNDETRVVRNRLDVV
                     KSNAYIGFILVVIILIIFLDFWSSFLIALSIPISFAITFVIMKLVNADINSISLAAMI
                     IALGMIVDQSIVISENALVYISYGKKKYEAILEGTMEVIVPVFASVLTTVLSFAPMFV
                     MTGVMGKFIYVIPVVVIASLVGSLLNSWFILPNQLSHVIKEKDPNAPEHRSWQDRFFD
                     YIAAPCRRIMKSVLKHSYITILVTIFLLIFSLWWGKNKILMNLFPPNGADTFFVYVEL
                     PDDATFNATEKTVHEIEDYIKKIPQNEVAFYTAKIGTNQSNDLAKPVGGEEHLAYIQV
                     TLVPSSRRKREAGEIMEELKTGVLASVKGAKELRFEVQKPGPPAGKPIEMHVHSDNDE
                     HRAMFVEKILNELKSTPGVSDVTSNAKLGREEYKLDINYQMLALAGLTVKDVASTLRI
                     AFDGVRATSVVRNNEEIDIRVRFPAEHRQDVKNVLLLHIRNREGKLVPIKTFAKLSKV
                     RAETAIHHTDGDVTTTVSAQTAVTTVPQAVIDGIIKKYSPELVKYPGVSFSYGGEAEK
                     SKESVKSLLIAFVGGILAIYL"
BASE COUNT          932 a          642 c          793 g          836 t
ORIGIN      
        1 aatattatct agagctttat gattcttctt ccaaatgggc cgaatatctg gctaagactc
       61 actcactgaa gacagtttcc ggcagttctc aggcatggtt cagcgtggat ggaattcatc
      121 tgaagtccag cattcgggat gttctggaga agtggaatga gcctgacaga aaaccggaga
      181 tggaagacgg tcttcaggtc tggtactatg acagcaggga agtgaagctt tccatgcgga
      241 acggatttgt agagcagatt atgctgtacg gggataaaag tccgtctgta gaggggctga
      301 aagtcggatc tgacagaact gctgtcgaaa aatttctcgg aaaaaatcct aaaactcaga
      361 acagatattt gatatatgaa aataaggaca aagtagggct gacctttgag gaaaacaaag
      421 tggaacgact gatcctgttt gagtgatttc agtcagacgt cggcattcct aagctgacag
      481 tattgcagag aaaataaagg gggcggttta cggaaggatg atttcgcccc gccgtcagct
      541 tgacagagtt tcgataaaga aaaatatgcg actatgagat actttatcga tttttttctg
      601 cgccgttctc tcctcgtaaa tatcctgctg gtcagcattt ttctgctggc tctggctgcc
      661 ctttccagaa tgaacaggaa taaatttcct gaagtggatc tggggacgat gatagtcacg
      721 accaaatacc ccggggcatc accgaaggat gtggagcaga atgtcacgag actgattgag
      781 gatgagctga aatcagtctc aggaattgat acattcaagt cggtttctgc agaaaacgta
      841 tccgttgtca ctgtcaacat tgacatcaat catccgaatc agcaggaagt gaaggatgac
      901 ataaggaggg cagtggacag agtttccggt ctgccttcgg aaattacgga aagaccgact
      961 gtgagagatc tcaaggcgtc cgagatgcct gtgcttgtcg tcggcgtatt cggtgatgcg
     1021 gaatacgatg aactcagaaa tatggccaag atcatcgaaa gggatctgaa ggggatcgac
     1081 ggagtcagta tggtcgacaa gtatgccttc cgcgacaagg aatttcatgt agatctgaat
     1141 cccaaaaaac ttgaagacct ttatgtttca ctgaatgatg tactgatggc gctaagcaat
     1201 cggaacattc gtgccacggg aggaagtctg gaatcctatt ccactcagag aaatattctg
     1261 actctttccc agtttgacag cattgaggat atccgtcagg tcatcatccg ttcggaactg
     1321 ggaggaggga gcgtcagagt gaaggacatc gctcaggttc aggatgattt tgaagatgaa
     1381 aaaatgagga cgatcttcaa cgggaagcag ggaattatgc ttgtcgtgaa aaaatccagc
     1441 caggcggaca tcatccgggt tgtggacaag atcaagctct atctggaaga aaaacagaaa
     1501 attattccgg aaaatgtaaa actcactgca gtcaatgatg agaccagagt cgtaagaaac
     1561 aggctcgatg tggtcaagtc caatgcgtat ataggattta tattagttgt cattattctc
     1621 atcatatttc tggatttctg gtcatccttt ctcattgccc tcagcattcc tatctctttt
     1681 gcaatcacat ttgtcattat gaagttggtg aatgcggaca tcaattccat ctcgcttgca
     1741 gcaatgatca ttgcgcttgg aatgattgtg gatcagtcca ttgtcatatc cgaaaatgct
     1801 cttgtatata tctcatatgg aaaaaagaaa tacgaggcga ttctcgaagg aacaatggag
     1861 gtgattgttc ctgtatttgc ttcagttctg acaacggtgc tttcctttgc tccaatgttt
     1921 gtgatgaccg gggtgatggg aaaattcatc tatgtcattc ctgtagtggt cattgcatcc
     1981 cttgtcggtt cgcttctcaa ttcctggttc attctgccga atcagctttc ccacgtcatc
     2041 aaggaaaagg atccgaatgc acctgagcac agaagctggc aggacaggtt tttcgactat
     2101 attgcggctc catgcagaag aatcatgaaa tctgttctga agcacagtta tattacaatt
     2161 ttagtgacaa tatttcttct gatattttca ttatggtggg ggaaaaataa gattctgatg
     2221 aatctttttc ctccgaacgg agcggacact ttctttgtgt atgtggaact tccggatgat
     2281 gccaccttca atgccactga aaagacagtt cacgaaatag aagactatat taaaaaaatt
     2341 cctcagaatg aggttgcctt ttatactgca aagataggca caaatcagtc caatgatctc
     2401 gcaaaacctg tcggcgggga agagcatctg gcctatattc aggttacact tgtcccctct
     2461 tccagaagaa aaagagaggc cggtgaaatt atggaagaac ttaagaccgg cgtgctcgct
     2521 tccgtgaaag gtgcgaagga actgcgtttc gaagtgcaga agccggggcc tcctgcaggc
     2581 aagccgattg aaatgcatgt gcattccgac aatgatgaac acagagccat gtttgtagaa
     2641 aaaatactga atgaactgaa gagcactcca ggcgtttccg atgttacatc caatgcgaaa
     2701 ctgggaagag aagaatacaa gctggacatc aattaccaga tgctggctct tgcaggactt
     2761 acagtaaagg atgttgcgag cactcttcga attgcatttg acggagtgcg tgccacttcg
     2821 gtagtgagaa acaatgagga gatagacatc cgtgtccgtt ttcctgcaga acacagacag
     2881 gatgtgaaaa atgtccttct gcttcatatc aggaacaggg aaggaaaact tgtgccgata
     2941 aaaacctttg caaaactttc caaggtgcgt gcagaaactg caatccatca tacggacgga
     3001 gatgtgacga ccactgtttc ggcacagact gcagtgacga ctgtacctca ggcggtgata
     3061 gacggcatca ttaaaaaata cagtcctgaa cttgtaaaat atcccggtgt cagcttttcc
     3121 tacggaggag aggcggaaaa gtccaaggaa tccgtcaagt ctcttctgat tgcatttgta
     3181 ggagggatac ttgcgatata tct
//
LOCUS       DAOQGJ010000205         3199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17709_length_3199_cov_3.999046, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000205 DAOQGJ010000000
VERSION     DAOQGJ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17709_length_3199_cov_3.999046"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PK683_04395"
     CDS             <1..1270
                     /locus_tag="PK683_04395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07718.1"
                     /translation="KVQQDGDYFLTSLLTDPLNFMNLNSETVKAEIFLKSKKQFVFRN
                     KHKELGGDINIIDEIHLSGRKYIIFMNADAMGKSLQGAGGALVLGAAFKSLILRAKKH
                     EDEEDQFPESWLKTMFEELGQIFETFNGSMLVSAVIGLIEEDTGFLYYVNAEHPFLVL
                     LRDGKAKFIETELQNRKLGITGFDDGVCIKLFQLRNNDILIAGSDGRDDIMMPDAGGI
                     KKMNHDETLFLSAVEKGEGNLNRIFQVLDDSGDLSDDLSMIRISFHGMNRTEEDSSEE
                     YKKIIYTAMQEKNNRLYHEAVSLFTHAMNTFSENIFILKQITSLYLRLGDFRNMLEYS
                     QRISKLAPEETRYLFYTSFAYYKLGDIAKAIYYSERVYLREPDNLKNIIHLIKLCKLS
                     GSRKLFDQYLNAGLKLDPDNHTLQAYRNLQ"
     gene            complement(1277..2350)
                     /locus_tag="PK683_04400"
     CDS             complement(1277..2350)
                     /locus_tag="PK683_04400"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNH07719.1"
                     /translation="MRSDLLKIQRRGAGINLPGRFDSVSNESFDDGWEIEEEKKTLKT
                     EIFFERAGSILNQIDSPDIPFEMTLNPYRGCEHGCIYCYARPNHGFMNLSPGLDFETK
                     IFAKKDAARLLEKEFSKKNYIPKIFAIGSATDAYQPIEKKLRITRSILQVCLDWKHPA
                     GIITKSSLIAGDMDILSKLAEENLIRVSITVTTLNPELQGKLEPRTASPKKRLETVRL
                     LSQAGIPVSVMFAPLIPGLNDEEMETVLEKSKEAGAENAGYVVLRLPFEVKDIFWEFI
                     DRNFPEKKKKIQTVLHSLFEGKDYSPVFGERMSGVGKFAKLYADRFRLAVRRLELNRR
                     REPLSLNKFRKIRDSQGQMSLFQ"
     gene            2728..3141
                     /locus_tag="PK683_04405"
     CDS             2728..3141
                     /locus_tag="PK683_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNH07720.1"
                     /translation="MNRGNQNSPKKTLLSCSDRTASEAVSIVLGILCIFFCNAVISYD
                     LEQYRIIKTGAGNWNKWRSANPGKHIDLSKADLKKAKLSGADLSGADLSFARLEKADL
                     SKANLYGASLEKANLLGAKLDNANFSEAKKQVAER"
BASE COUNT          949 a          642 c          664 g          944 t
ORIGIN      
        1 caaggtgcag caggatggcg actattttct cacttcgctt ctgactgatc ctctgaattt
       61 tatgaatctg aacagtgaaa cagtaaaggc tgaaatattc ctgaaaagca aaaagcagtt
      121 tgtgttcaga aacaagcaca aggaactcgg cggggacatc aatatcattg atgaaattca
      181 tctgtcaggc agaaagtata tcatctttat gaatgctgat gcaatgggaa aatctctgca
      241 gggggccgga ggtgctctgg ttctcggggc tgcattcaag agcctgatac tgagagcgaa
      301 aaaacatgag gatgaggaag atcagtttcc ggagagctgg ctcaagacaa tgtttgaaga
      361 gctgggacag atatttgaaa cattcaacgg atcaatgctt gtatctgcag tcataggtct
      421 gatagaggag gatacaggat ttctttatta tgtaaatgca gagcatcctt ttcttgtgct
      481 ccttcgggac gggaaggcaa aattcataga aacggaactt cagaacagaa aactgggcat
      541 taccggattt gatgacggag tatgcattaa gctttttcaa ttgcggaata atgatatttt
      601 aattgcgggt tctgacggaa gagacgatat tatgatgcct gatgcaggcg gaattaaaaa
      661 aatgaatcat gatgaaacac tgtttctttc agctgtggaa aaaggagagg gcaatctgaa
      721 ccgtattttt caggtacttg atgattccgg ggatctgagt gatgatctgt ctatgatcag
      781 aatatccttt cacgggatga acagaacgga agaagattct tcggaggaat acaaaaagat
      841 catatacact gccatgcagg aaaagaacaa caggctttat catgaagcag taagcctgtt
      901 cactcatgca atgaatacat tttcggagaa tatattcatt ctcaaacaga tcacaagtct
      961 gtatctccgg ctgggcgatt tcaggaatat gctggagtat tcgcagagaa tttcaaaact
     1021 ggctcctgag gaaacgagat atctttttta tacttccttt gcatattata aacttggtga
     1081 tattgccaaa gcaatatatt attcggagag agtatatctc agagagcctg acaatctgaa
     1141 aaacatcatt catctgatta aactgtgcaa gctttccgga agcagaaagc ttttcgatca
     1201 gtatctgaat gcaggactga agcttgaccc ggacaatcat actctgcagg cctacagaaa
     1261 tctgcaataa gcagcattac tgaaaaagac tcatctgtcc ctgactgtct ctgatttttc
     1321 tgaatttatt cagggacaga ggctcccgcc tgcggtttaa ttccagcctc ctgactgcaa
     1381 gcctgaatct gtctgcgtaa agttttgcaa atttcccgac accgctcatc ctctctccga
     1441 aaacagggct gtagtccttt ccttcaaaca gactgtgcag aaccgtctga atttttttct
     1501 ttttttcggg aaaattcctg tcaataaatt cccagaaaat atcctttact tcaaacggca
     1561 gacgaagtac aacgtatccc gcattttctg ctccggcttc tttagatttt tctaatacag
     1621 tttccatttc ttcatcattc agaccgggaa tcagcggggc aaacattaca ctgactggaa
     1681 ttcctgcctg cgaaagaagt ctgacagtct ccagtctttt tttaggtgaa gcggttctgg
     1741 gttcaagttt tccctgcagt tcaggattca gtgttgtgac tgtaatgctg accctgatca
     1801 gattttcttc agccagtttg gaaaggatgt ccatatcccc tgcaatcagt gaagactttg
     1861 taatgattcc tgcaggatgt ttccagtcca ggcagacctg aagaatactt cttgtgattc
     1921 tgagcttttt ttcaatcggc tgatatgcat cggttgctga acctatggca aatattttag
     1981 gaatataatt ttttttagaa aattcctttt ccagaagtct tgctgcatcc ttctttgcga
     2041 agatctttgt ttcaaaatcc agtccgggag aaaggttcat gaatccatga ttcggtcttg
     2101 cataacagta aatgcatccg tgttcacatc ctctgtacgg attcagagtc atttcgaacg
     2161 ggatgtccgg agaatcgatc tgattcagaa tactgcccgc cctttcaaaa aagatttccg
     2221 tcttcaatgt tttcttttct tcctcaatct cccatccgtc atcgaaggat tcattggata
     2281 ctgaatcaaa tcgacccggt aaatttattc ccgcacctct tcgctgaatt ttcaaaagat
     2341 cagaacgcat tccaaatcct ccccagagca ataagacgat aattaactat atttatatta
     2401 agtaaatata aatatagttt tattttttga ttcagtcctc ctgaaaaaca gggatttttc
     2461 tgcctgattc ttttcccgtc cttggacgga agcaggagaa atcagtcgtt ccagacatac
     2521 acctagattc ttttttcaga aaacggttcc ctaaaataag gaagaatccc tatgaaaaaa
     2581 agagattttt ttataaagct gatttacttt ttttctaggg aacagcatat taccttttac
     2641 gcataattgt gattcttggc tgatcacaga tgctatggat cattttcagt ctgtacagat
     2701 aaaactgact gaaaggaaag gtttttaatg aacaggggca atcaaaactc accgaaaaaa
     2761 actctgctct cctgcagtga cagaacagct tcagaagcag tttccattgt gcttggtatt
     2821 ctatgcatat ttttctgcaa tgctgtgatt tcttatgatc tggaacagta tagaattatc
     2881 aaaacgggtg cgggcaattg gaataagtgg aggtcggcca atcctggaaa acatatcgat
     2941 ttgtccaagg ctgacctgaa aaaggcaaaa ctctccggtg ctgatctttc cggtgctgat
     3001 ctttcctttg caagactcga aaaggctgat ctgtccaaag caaatcttta tggagcgagt
     3061 cttgagaagg caaatcttct cggagcaaaa ctggacaatg caaatttcag tgaagccaaa
     3121 aaacaagttg cagaacgctg atctagactg tctaatttac caggagtcaa tatgaaaaag
     3181 aaattaactt acagccttc
//
LOCUS       DAOQGJ010000206         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17762_length_3193_cov_2.195029, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000206 DAOQGJ010000000
VERSION     DAOQGJ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17762_length_3193_cov_2.195029"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PK683_04410"
     CDS             complement(<1..623)
                     /locus_tag="PK683_04410"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HNH07721.1"
                     /translation="MKKLLIVTIFIIAFTHNCMTFFTSYNWKKEPSSRIYSGTAANVQ
                     LIFNGPIQGQWSVLFPMALIDFPFSFTADTVLLPYHGFYAILYGLAEISKEDSNTPKR
                     NEYEKKYPAYSKFEKAIKDNNLELWNDKEELKKDEELIFDNSAYEMLHRSNVSWPCFS
                     VDWLIPEYFIPQTFKNFYSPLENRMYKDEFPYTCYFIAGAQTSEKNG"
     gene            complement(655..1062)
                     /locus_tag="PK683_04415"
     CDS             complement(655..1062)
                     /locus_tag="PK683_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07722.1"
                     /translation="MFGTLPIPDWSVAIDITLGWSFLSSDESNVMLIDPKGITTVYRM
                     GLVSSDALCIPAVRACLRLRMENSSWVVNLENFKQLPSSTVIQGVRISHLKGILYLSK
                     DHKSIIWGNNIESDGGPAFVFRRINIDEIKVED"
     gene            complement(1205..1420)
                     /locus_tag="PK683_04420"
     CDS             complement(1205..1420)
                     /locus_tag="PK683_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07723.1"
                     /translation="MDIKEILKDREARGLIISVYGHFAAFAVFFLLAVNLPNNESIIY
                     FWIVMTVSILLGLLRVKKRSGNDMLMR"
     gene            complement(1462..2325)
                     /locus_tag="PK683_04425"
     CDS             complement(1462..2325)
                     /locus_tag="PK683_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNH07724.1"
                     /translation="MNIRNIISALLLGLAVWAGVAYFSARFLVRPNHISIYNIDKIDS
                     KKAESMEFMTEDNFRISAWFVRNSEKRVIVLLSGIRGNRLAQISRAEFYLKKGFSVLM
                     PDLRGTGKSEGDLISFGWHERKDLLACILELKRLGFSKISVDGQSLGAAAAVYSMSDY
                     ADYEFIILESCYDNITNAFRNRINKFPVPHFVFRPVEFFTGKMIGASVQELVPDVLIA
                     KIASPVLFLAGDSEYQIPTEETVKLFERSESKFKRLHFFKGAEHEDFHVRYRGEFEKT
                     VSDFLNSMNVQ"
     gene            complement(2433..2969)
                     /locus_tag="PK683_04430"
     CDS             complement(2433..2969)
                     /locus_tag="PK683_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07725.1"
                     /translation="MRSFFSKRTFEKLFTVFCFILSAEINSKSSAVRSFASQFRKDYT
                     VEKRKIANQKESFSSFSPRQLKLEIYREFKSRKPILSGSEKIFLRFSLSVLKYRDRQS
                     ALEAFAGFKKNPGTRFIKSPTLIVLKKKRIYDLSGKCLYSGKNWNDIESKLLSSLNRK
                     KPVDSKNIYKIPCGGKRS"
BASE COUNT          978 a          668 c          540 g         1007 t
ORIGIN      
        1 tatccatttt tttcagaagt ctgtgctcca gctataaaat aacatgtata aggaaactca
       61 tctttgtaca ttctattttc taatggtgaa taaaagtttt taaatgtctg aggaataaaa
      121 tattctggta ttaaccaatc tacagaaaaa catggccaag aaacgtttga tctatgtagc
      181 atttcatatg ctgaattatc gaatattaac tcttcatctt tttttaattc ttctttatca
      241 ttccataatt ctaaattatt atctttaatc gctttttcga atttgctata ggcaggatat
      301 tttttttcgt attcgttccg cttaggtgta ttcgaatctt ctttcgaaat ttctgccaat
      361 ccatacagga ttgcataaaa accatgataa ggtaaaagaa cagtatccgc tgtaaatgaa
      421 aaaggaaaat caatcaatgc cattggaaaa agaaccgacc attgaccctg gataggtcca
      481 ttaaatatca attgcacatt cgctgcagtt cctgaataaa tacgagagga aggttctttt
      541 ttccaattat atgaagtgaa aaaagtcata cagttatgag tgaatgctat tataaaaatt
      601 gttactatca aaagtttctt cataattttc tccatcgaag tgaaacagaa tgccctaatc
      661 ctccactttt atttcatcga tattaattct acggaacaca aaggcaggtc cgccatccga
      721 ttctatatta ttaccccaaa tgatactttt atgatctttg gataaataga ggattccttt
      781 taaatgagaa attctgacac cctgaattac tgtagaagaa ggaagttgtt tgaaattttc
      841 taaattcaca acccaagaag aattttccat tctaagccta aggcaggctc gtactgcagg
      901 aatacataga gcatcggaag aaaccaaacc cattcgatag acggttgtta tcccctttgg
      961 atctatgagc atcacattgc tttcatctga tgagagaaaa ctccatccaa gcgtaatgtc
     1021 gatcgccacg ctccaatcag gaatcggcaa tgtcccgaac attgttgtaa atgagcatat
     1081 actaagcaac ttcaagtaat acaaagctat tcaactgaga tattctgtca agcagaacag
     1141 gaatcgactt gtattccttg actcgtctga aattctgttc actttcgata agggctgacg
     1201 aaattcagcg cataagcata tcgtttccag accgcttctt cacgcgcaga agtcctaaca
     1261 ggatggaaac agtcataaca atccagaaat aaattataga ttcattattc ggcagattta
     1321 ctgccagaag aaagaaaact gcaaatgcgg caaaatgtcc gtaaacagaa atgatcagcc
     1381 cccttgcctc cctgtccttt aaaatctctt ttatatccat gatagtttcc ttttttctgc
     1441 aatctctgag ctgcattccg attattgaac attcattgaa ttcaaaaaat cggaaactgt
     1501 tttttcaaat tccccccggt atcttacgtg aaaatcttca tgttctgcac ccttgaaaaa
     1561 atgcagtctt ttaaacttgg actcagatct ctcaaaaagt tttacagtct cctctgtcgg
     1621 tatctgatat tcactgtctc ctgccagaaa cagaacggga gatgcaattt tggcgatcag
     1681 tacatccgga accaattcct gcactgatgc accgatcatt ttcccggtaa agaactccac
     1741 cggcctgaag acaaaatgcg gaacaggaaa tttattgatc ctgtttctga atgcatttgt
     1801 aatattatca taacaggatt ccaggatgat aaactcatag tcggcataat cagacatcga
     1861 atacacagca gctgctgctc cgagagactg tccgtccaca gagattttac tgaatcccag
     1921 tctcttcagc tcaagtatgc aggccaggag atccttcctc tcgtgccagc cgaaactgat
     1981 cagatcacct tcacttttcc ctgtccctcg gagatcgggc atcagaactg aaaaaccttt
     2041 cttcagataa aactcagccc gtgaaatctg ggcaagccgg tttcccctga ttccggaaag
     2101 aaggacaatc actctctttt cagaatttcg gacaaaccag gcggatattc tgaaattgtc
     2161 ttctgtcata aattccatgc tttccgcttt cttgctgtca atcttatcta tattatatat
     2221 acttatatga ttcggcctga caagaaaccg tgcagaaaaa tatgcaacgc ctgcccagac
     2281 tgcaagtccc agcagaagag cggaaataat atttcttata ttcataaatc tgcagatcat
     2341 ccttcgtgaa ggatctctaa ggaatactga tgcatttcag gccggatatc ctctgatcag
     2401 cagatgcaaa agtacattga aaaccgctcc tctcaagatc ttttccctcc gcagggtatt
     2461 ttataaatat ttttagaatc cacaggtttc tttctgttca gagaactgag aagcttggat
     2521 tctatatcat tccagttttt tccggaatac agacattttc cgcttaaatc ataaattctt
     2581 ttctttttca aaacaataag agtgggagat ttgatgaatc tggtccctgg atttttttta
     2641 aagcctgcaa atgcttccag tgcagactgt ctgtcccggt acttcagtac ggagagagaa
     2701 aatcggagaa atattttttc ggatccggaa agaatcggtt ttcttgattt gaattctcgg
     2761 tatatctcga gcttcagctg cctgggactg aaactggaaa aagattcttt ctgatttgca
     2821 atttttcttt tttcaacagt ataatccttt ctgaactgag aagcaaatga tctgaccgcc
     2881 gaagatttag aatttatctc tgccgataga ataaaacaga atactgtaaa caatttttcg
     2941 aatgttcttt ttgaaaaaaa actcctcatc ttccttcctg tctgcaatga actgcttaaa
     3001 tatctctatg attttttttt ccggataatt tttctgcaag aggaaatcag ttacagtctc
     3061 tgaaatttta tgtttcggat cagcgtcttt tttccaatca gcagcaggtt ttaaggcata
     3121 ttctcagcac tgcgcattga tttaccaaaa acagtatcag tgtgcctatt ataatcctct
     3181 tatatgatga ttc
//
LOCUS       DAOQGJ010000207         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17774_length_3192_cov_3.058017, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000207 DAOQGJ010000000
VERSION     DAOQGJ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17774_length_3192_cov_3.058017"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PK683_04435"
     CDS             complement(<1..234)
                     /locus_tag="PK683_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07726.1"
                     /translation="MSFYFPPDPEKIISAAESLAEAAVLEKDFLLIGHRLGNFRHYPL
                     NVNRKRYLKDYRNTSELMIKSFTEYRLNALELDL"
     gene            419..721
                     /locus_tag="PK683_04440"
     CDS             419..721
                     /locus_tag="PK683_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07727.1"
                     /translation="MNKIRLGGLLIMLIGILGNVYNWVQVMNAGQFFEKASILFPFFA
                     FLGLSVIIYPMTKEERISRFGSDQIPLRGLPAGQKILAGVGLLIGIIQWLWLEGLP"
     gene            777..1601
                     /locus_tag="PK683_04445"
     CDS             777..1601
                     /locus_tag="PK683_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07728.1"
                     /translation="MIISAASVYSEDADLLNKSFSSSVLSGDLKFYEDLKEKNFPLNE
                     ENKKNFSRISAETCFEKGYKEDIYVWKTSPVYLNCLKSLKGVWSISEPKNRKLMTSVF
                     VSFLTHPGHPEYDEFFKDRNLMQTYKEHREELRKSEFENDSESFISGMFDFFLDKAEI
                     LENLFAGNEVYFKSAEENPLNEKMQEIIDSYCNSPKYFNECLKSLHGGMLNERDELRK
                     SFYSASVNMILSGRFYQERKIPEWFTERINSIEEKNKLNILHLNSLKKVLRKRTHY"
     gene            complement(1608..2924)
                     /locus_tag="PK683_04450"
     CDS             complement(1608..2924)
                     /locus_tag="PK683_04450"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH07729.1"
                     /translation="MAENYEKYRAVNSARIFISGFDLTLIILMKVFDPVLIWGGTLHL
                     FFSILWMFLIETDIFKERKYRNSGYIPAFIDISAATIIILMTGNIHSYLNVGYFMITA
                     VSSVQARKTFGLSIAVLCFLQYFVSGLLVYFSIISNVNVFADSTTRITLTSLLIASVW
                     VFIGLFTVHSIIHSFVMKSRDLAEKAEKEREKSEKLLTTIQKELQFAKRIQKKLLPND
                     MFTYDSLKIRSIYRPMAEVGGDLFDILNLGQGRIRIFLADATGHGVEAALVTMLIKSE
                     LEGIKTESESPAALLGTMNDIIHQKYSSLSLFFSCIAADIDLEKQRLIYASAGHPDQY
                     FMSSDSVTALPKTGKLVGVLSSQVYEEKTLDFRPGNRVLLFTDGLFEEFNLHNEEYGE
                     ARIFSILKENLREDFDTAVRLIEKDLEKFLNSAPIQDDITIIGIQG"
BASE COUNT          961 a          601 c          669 g          961 t
ORIGIN      
        1 gagatcaagc tcaagagcat tcagcctgta ttctgtaaat gactttatca taagttcact
       61 tgtatttctg tagtctttca gatatctttt tctgtttaca ttcaaggggt aatgtctgaa
      121 atttccaagc ctgtggccga tcagaagaaa gtctttttcc agaactgcgg cttctgcaag
      181 tgattctgcg gcagatatta ttttttcagg gtcaggagga aaatagaagc tcatagggtt
      241 ttcactcctg ctccattcgg gtctgtcttg ttcataggtt cagaatagaa gactcctgtt
      301 acgctgtgat tacagtcgga taaagattgt tcatttcgtt tttccggaaa aaaacgcatc
      361 tgtattgaaa aatctgcggc ctgcgtctgt actgtctttc atgaaggttt ttagcgaatt
      421 gaataaaatc aggctgggcg gactgctgat tatgctcata ggcattctgg gaaatgtcta
      481 caactgggtt caggtaatga atgcaggtca gttctttgag aaggcaagca ttctttttcc
      541 tttctttgca tttttggggc tttcggttat tatttatccg atgacaaaag aagaaagaat
      601 ttccagattc ggttcagatc agattccttt gagaggtctt cctgcagggc agaaaatact
      661 tgcaggggtc ggacttctga tcggcataat ccaatggctc tggcttgaag gactgccttg
      721 attttttata tgaacagcaa atgcatcagg ctgcataaat gaagatattc ctgtttatga
      781 taatttcagc agcttctgtt tattccgaag atgcagatct tctcaataaa tctttcagca
      841 gttctgttct gtccggcgac cttaagttct atgaggatct gaaggaaaag aattttcctc
      901 tgaatgagga aaataaaaaa aacttcagca ggatttctgc agaaacctgt tttgaaaaag
      961 gatacaagga agatatttat gtatggaaaa cctcgcctgt ctatctgaac tgcctgaaga
     1021 gcctgaaagg agtctggagc atttcagaac caaagaatcg taaactgatg acttctgtct
     1081 tcgtaagttt tctgacccat ccgggacatc ctgaatatga tgaattcttc aaagacagaa
     1141 atcttatgca gacatataaa gaacacagag aggaacttag aaaatcggaa ttcgaaaatg
     1201 attcagaaag cttcatatcc ggaatgttcg atttttttct ggataaggca gagatactgg
     1261 agaatctttt cgcaggaaat gaagtgtatt ttaagtcagc tgaagaaaat cctctgaatg
     1321 aaaaaatgca ggaaataata gattcgtact gcaattcccc taagtatttc aatgaatgtc
     1381 tgaagtcgct ccacggagga atgctgaatg aaagggatga attgaggaaa tctttttatt
     1441 ccgcctccgt aaatatgatt ctttcaggaa gattttatca ggaaaggaaa attccggaat
     1501 ggtttacgga aaggattaat tccatcgagg aaaagaataa actgaatatt cttcatctga
     1561 acagtctgaa aaaggtgctg agaaaaagga cgcactatta gactttctta gccttggatt
     1621 cctatgatgg taatgtcatc ctgaataggt gcggaattca gaaatttctc aaggtctttc
     1681 tctataaggc ggactgctgt gtcaaagtct tctctcagat tctccttaag tatggaaaaa
     1741 attcttgcct ctccgtattc ttcattgtga agattgaatt cttcgaaaag cccgtcagta
     1801 aaaagaagta ccctgtttcc tgggcgaaag tccagagtct tttcttcata cacctgactg
     1861 cttaagacac ctacaagctt ccctgttttg ggcagggcag tcactgaatc tgaactcata
     1921 aaatactgat ccggatgacc cgctgatgca tagatgagtc tctgtttttc caagtcaatg
     1981 tctgcggcaa tgcatgagaa aaaaagactc aatgaactgt acttctgatg aataatgtca
     2041 ttcattgtgc cgagaagcgc tgcaggagat tctgattctg tcttgattcc ctccagctca
     2101 ctcttgatca gcattgtgac aagagccgcc tccactccgt gtccggttgc atcagccaga
     2161 aatatcctga ttctgccctg accgagattc agaatgtcaa aaagatctcc tcctacttcc
     2221 gccataggtc tgtagattga acgtattttc agtgaatcat aagtaaacat atcatttgga
     2281 agaagcttct tctgtattct tttggcaaac tgaagctcct tctgaatcgt ggtcagaagt
     2341 ttttccgact tttctctttc cttctctgct ttttccgcca gatccctgct cttcattaca
     2401 aaactgtgaa tgatggaatg cactgtgaaa agaccgatga atacccagac agaagcaatc
     2461 agaagagaag tcagtgttat gcgggtagtg ctgtcagcga atacattgac attggatata
     2521 atgctgaaat atacaagaag accgctcacg aaatactgaa gaaagcagag aactgcaatg
     2581 gaaagtccga aagttttcct tgcctgcact gaggagactg ccgtaatcat aaaatatcct
     2641 acgttaagat aggagtgaat gtttccagtc ataagtatga ttattgtagc ggcacttatg
     2701 tctatgaagg ccggaatgta tcctgaattc ctgtattttc tctctttgaa aatatctgtc
     2761 tcgatcagga acatccagag aattgaaaaa aacaggtgaa gtgttcctcc ccaaatgaga
     2821 acaggatcaa aaactttcat caggatgatg agagtcaggt caaatcctga gatgaatatt
     2881 ctggctgaat tcaccgctct gtatttttca taattctctg ccatagggat aatatgtaat
     2941 ttaattatgc atctgcaagc actaaaatcc ttcagtgttc cgaattttta tccgaataaa
     3001 ttaatttccc ggattttgaa aatatactat acaaatattt acattaataa agaaaagtcc
     3061 tatgaggcgt gaacatcaga attctcttga agtaaggaaa agaggagagg tctacagctg
     3121 ggacagaaaa ggacttcttt ttcttaaagg aaaggaagga ttcggaatcg cgcatatcct
     3181 gtgtgcagaa ct
//
LOCUS       DAOQGJ010000208         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17798_length_3189_cov_4.532227, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000208 DAOQGJ010000000
VERSION     DAOQGJ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17798_length_3189_cov_4.532227"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PK683_04455"
     CDS             <1..368
                     /locus_tag="PK683_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07730.1"
                     /translation="QKVKSESNRQGFALEQLADKLDYSLDSLRLLFSNFAKSWYGTKK
                     IRPNPEKMLKFLTDNSDSLSSDYKGEVQTDRYRDENGKYTNQDGASEYYKREWEEKYQ
                     KAIQKNYTQEELEEAARIF"
     gene            368..1141
                     /locus_tag="PK683_04460"
     CDS             368..1141
                     /locus_tag="PK683_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07731.1"
                     /translation="MKNIEELKGLADKMFARASVNWKPEPRFQIKKNRIEVIEKYNND
                     IRPKLNNNDMQMLIREFYQINKKGFRFKGILKLPSEPILIIGETGLGKTYLLKTFMAY
                     CKDRQEQGTRIRKVIAKQSHYSDNGRLKRNRFAQGAIHNYAFTYLSENDFRNYAVKKN
                     DYEIDLILDRFMDKSLKYIFLDELFYKSNWNFDGYYSHAEGYFKQLWDTILDARHSGK
                     IIVGTTNNNPNGKYVVNVAVIQRRIAETFTKQIVLEGKK"
     gene            1138..1329
                     /locus_tag="PK683_04465"
     CDS             1138..1329
                     /locus_tag="PK683_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07732.1"
                     /translation="MNQEQIQFYRDMALSVYEMLAKAGDESDSKNADMLVRCAEFHMK
                     AILSDIQNRDRYVLQSAKG"
     gene            1304..1693
                     /locus_tag="PK683_04470"
     CDS             1304..1693
                     /locus_tag="PK683_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07733.1"
                     /translation="MSCKVQRDDMGNYNIDHTAFEKSARNAKRSIILSEQVQEHSYIT
                     LHERKEGRITGRKLLCQVANCETVYEAGLMVGEYYPSGQVPMKLPEFYLVEYVPMLTD
                     KQTVLELKSTLESEPDLEAAHRYIFGA"
     gene            1858..2151
                     /locus_tag="PK683_04475"
     CDS             1858..2151
                     /locus_tag="PK683_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07734.1"
                     /translation="MKILIFLKRSNPMTDKIPMPKVKGLKVTQKRIERERAYYALSAE
                     GKMFADIDKILNEIRDEYTIMNLEGMCTTQDIIRCLNIDILRLMKFREVLQRI"
     gene            2392..3144
                     /locus_tag="PK683_04480"
     CDS             2392..3144
                     /locus_tag="PK683_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07735.1"
                     /translation="MSDLIYGHNTKVGYKVFLPDWKSKSGDLPPFEVGKEYTHDGELE
                     ICKSGFHYCENLIDCFNYYEWDIRNKVCLIHVDDSVSMSAGEKSVTGKFTIIRELDWN
                     EVFKLCNTGLNCTGYGNSGNGNSGNGNSGDWNSGYWNSGFFNSKTPVCSIFDDPTNLT
                     HDDVFKSEWYLYLRNLYFPLNIFVLEEKMSKKEKEKYPHCVTLGGYLKTLTYKEAWGI
                     ALKDISENEKNILRSIPNWDAEKFYEITGVRI"
BASE COUNT         1135 a          528 c          722 g          804 t
ORIGIN      
        1 gccaaaaggt caaatcagaa tccaaccgac aaggctttgc acttgaacag ttagcagaca
       61 agctggatta cagcctagac agtttgagac tcctattcag caatttcgca aagtcatggt
      121 atgggacaaa aaagatccgg cctaatcctg agaagatgct aaagtttctg actgacaatt
      181 ccgattctct cagcagtgat tataaaggcg aagttcagac cgaccgatat agagatgaga
      241 acgggaaata cactaatcag gacggtgcaa gcgaatacta caagagagaa tgggaagaga
      301 agtatcaaaa agcaatacag aaaaattaca cacaggaaga gcttgaagag gctgcaagga
      361 tattctgatg aaaaatattg aagaacttaa gggcttggct gataaaatgt ttgcgagggc
      421 ttctgttaac tggaaaccgg aacctagatt tcagatcaag aagaaccgga ttgaagtcat
      481 agagaaatac aataatgaca ttaggccgaa actgaacaat aacgatatgc agatgcttat
      541 cagggaattt tatcaaataa ataagaaagg ttttcgattc aagggaatcc tgaaactgcc
      601 atcagagccg attctgatta tcggagaaac tggacttggc aagacttatc tgctgaagac
      661 attcatggca tactgcaaag acagacagga acaggggaca cggatcagaa aggtcattgc
      721 aaagcagtcg cactactcag acaatggacg gcttaaacga aaccgatttg cacaaggtgc
      781 aattcataat tacgccttca cttatctttc tgaaaatgat ttcagaaact atgctgtcaa
      841 gaaaaacgat tatgagattg atctgattct tgacaggttc atggacaagt cattaaaata
      901 tatttttctc gatgaacttt tctacaagtc aaactggaac tttgacggct attattcgca
      961 cgctgaaggg tattttaagc agttgtggga tactatctta gatgcaagac attcaggaaa
     1021 aattattgtc ggcaccacta acaacaatcc gaacggaaaa tatgttgtga atgtggcagt
     1081 cattcagcga aggattgcag agacgttcac aaagcagata gtattggagg gaaagaaatg
     1141 aaccaggaac aaattcaatt ttacagggat atggcattaa gcgtttatga gatgctcgct
     1201 aaggcggggg atgaatcaga ctcaaaaaac gctgatatgc ttgtaagatg cgcagagttt
     1261 cacatgaaag ccattctgag tgatattcag aacagggaca gatatgtctt gcaaagtgca
     1321 aagggatgat atgggcaatt acaatattga ccatactgct tttgaaaaat ctgcaagaaa
     1381 tgccaaacgg agcatcatcc tgtcagagca ggtgcaggag cattcttaca taaccctgca
     1441 tgagcgcaag gaagggcgga tcacaggtag aaagctcctc tgccaagtgg caaactgcga
     1501 gacagtatat gaagccggtc tcatggtcgg tgaatactat ccatccggtc aagtgccaat
     1561 gaagctgcct gaattctatc ttgtcgagta tgttcctatg ctgactgata agcagacagt
     1621 ccttgaactc aagagcacac tggaatcaga gccagacttg gaagctgcac acagatatat
     1681 ttttggagcg taatggaatg agagtttact atcaagcaag caataaaaat tattttaaca
     1741 gcattaaagg gaatacatat catgaatgca ctaaatgtga atcagtcatg aaaccgaatg
     1801 acaaggtgct taaaacaata gatacgtggc aaatatcagt atgcaagttc tgcgaaaatg
     1861 aagattttaa tatttttaaa gaggtcaaat ccaatgactg ataaaatacc gatgccgaaa
     1921 gtgaagggtt tgaaggtcac tcagaaacgg attgaacggg agagagctta ctatgcactg
     1981 tctgcagagg gcaagatgtt tgcagacata gacaagatcc tgaatgagat tcgagacgaa
     2041 tacactataa tgaaccttga gggtatgtgc actactcagg atataataag atgcctgaac
     2101 atcgacatac taaggctgat gaaatttaga gaggtattac aaagaatatg attgaagaac
     2161 atttcaaaga attccaaaag aaaaacagat ttcgcacaac ggataaagaa cctacatgct
     2221 attcatgcaa gggattcgat tcagtagacg gacatgatta catctgcctg gcatctgaga
     2281 aagattatca gtatatgaaa atttccatta ctactgtatg taataatttt tcaccggcat
     2341 agtaaagtct gaagaaaaat tgacgataag aaacatataa aagaggatga tatgagtgat
     2401 ttaatttatg ggcataatac aaaggtcggg tataaggtgt ttctgcctga ctggaaatct
     2461 aaatcaggag atttgccgcc atttgaagta gggaaagaat atacgcatga tggtgagctc
     2521 gagatatgca agtcggggtt ccattattgt gaaaatctga tagattgctt taactattac
     2581 gaatgggaca tcagaaataa ggtttgcctg attcatgtcg atgactcagt atcaatgtct
     2641 gcaggagaaa agtcagttac gggtaagttt acaattattc gggaacttga ctggaatgaa
     2701 gtttttaaac tttgcaacac tggattaaac tgcactggat acgggaactc agggaacggg
     2761 aactcaggga acgggaactc aggggactgg aactcagggt actggaactc agggtttttc
     2821 aattcaaaaa ctccggtatg ttcaattttt gacgatccaa caaatcttac acatgatgat
     2881 gtttttaaat cagagtggta tttgtatttg agaaatttat atttcccatt gaacattttt
     2941 gttcttgaag agaagatgtc aaaaaaagaa aaggaaaaat acccgcattg tgttactcta
     3001 ggcggatatc taaaaacgct gacttataag gaagcatggg gaattgcact aaaagatatt
     3061 tctgaaaatg aaaaaaatat tttgcgatca attccaaact gggatgctga gaagttttat
     3121 gaaattactg gagtgagaat ttgaaagaaa gaccaattga caaagcaatg aaagcaagta
     3181 atgtatacc
//
LOCUS       DAOQGJ010000209         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17808_length_3189_cov_2.447352, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000209 DAOQGJ010000000
VERSION     DAOQGJ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17808_length_3189_cov_2.447352"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..663
                     /locus_tag="PK683_04485"
     CDS             433..663
                     /locus_tag="PK683_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNH07736.1"
                     /translation="MTEPEILTEKVITQNKTFLVDLKRNQAGYYLKVSEWSNSKKSSI
                     FIPEEGVEKMIEILSRFKNSMAENQGMKTLVG"
     gene            704..1486
                     /locus_tag="PK683_04490"
     CDS             704..1486
                     /locus_tag="PK683_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNH07737.1"
                     /translation="MKFKSFLLTAVLAFVSYTSVSAQATTPAPAKQPAPAGGNNAPAG
                     GKLGDALERVTLEDFEMAEDWRAKSTTPLGETKTLKMIQRGLIKDVFDEKSMPDTKDD
                     KGNYNPATDNQDEKIGKNHVLGVKTYFANRGFDRVEVAPPQEFIIKGKARQVSIWVLG
                     RKYRHTLYAKFRDYRGNTHNIRLGRLDFFGWRRLTAPIPGFIPQSARYSLLDKNIRFV
                     SLFVTSDVHEVGGEFYFYVDDLEVRTDKQESVYPGFEIKDNW"
     gene            1513..2553
                     /locus_tag="PK683_04495"
     CDS             1513..2553
                     /locus_tag="PK683_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNH07738.1"
                     /translation="MNKKKAILAGIASVGITLAGVAGFAFAQQKGVKTSNGNDLSQGE
                     LRAITLENWDKTDLWQVFTDKDSMTYPDNLEQAKYDPKLAPTSMAMREVKYLDGAYPR
                     DVRYLDTRKDEEKKNLKVLGVKFQFTYSGNNEVTIRPPRTPDYQVKRYRTFLTGNEAN
                     LDRNEAKKSPEDRKVLDLEYKIYGIDLPSVAKALSVWVCGRGNDYDLEGWIEDYKGDT
                     HIVKFGSVGFVGWRPMTAKIPVSIPQFSSNYPATRTAVFKQFKLRSNYRSSGDPVILF
                     FDEFRVLTDTFEVHFDGANLDFDDEDCANKAKLDAMIDKRTPGDPYRTVKCGDKGGAP
                     AKGSAPAAPTKQ"
     gene            2699..>3189
                     /locus_tag="PK683_04500"
     CDS             2699..>3189
                     /locus_tag="PK683_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreAB"
                     /protein_id="HNH07739.1"
                     /translation="MSQDTAVLPDKASGEFQKLTALINEEIYVRVDAGNVPVTKFKIY
                     DDLIQHYKQLGKLTEANQLMKEHLNDHQDSISSRYMMGIISLMQNKLEDSNHLKTLLE
                     QLKGHGKWSIIEHVADQILLFGEQRMALKYKAEALEKQNKNKELKFVLEKLAKHDRKN
                     PEIA"
BASE COUNT         1063 a          572 c          666 g          888 t
ORIGIN      
        1 aaatgaatag tagatttttc atgcctgcct ggtattctcc tctaatatgt caatttctga
       61 cttccggaac attcttgtat tcggggcaat tccagttctg aaatcagatg tccgatgaat
      121 gcggggactc caatagatca gtctaaaaga tatcggcagg attcaagaat tattccccaa
      181 aaatagttaa tatccggatt ttttctcaaa tttctgcaaa aatgaaaaca ttgattttac
      241 tggcgtataa tcctgtcttt cctcagcctt caacggctca atcagtttca ataaaaaaag
      301 agagtttttc gatttttgta aaaaaacctt tgtactgttt tttttgggtt gctaagtttt
      361 gatttagatt taccgataaa tatagaagca aattttactg accggaaatt tgtacatttt
      421 gaggaagcag ggatgactga accggaaatt ttgacagaga aagttattac gcagaataaa
      481 actttccttg tggatttaaa gagaaaccag gctggttatt atctgaaagt ctcagaatgg
      541 tcaaacagca aaaaatcttc gattttcata cctgaagaag gggtagagaa aatgatagaa
      601 attctctccc gattcaaaaa ttcaatggca gagaatcagg ggatgaaaac actggtcggt
      661 tgaaactgtg tatttttata gaaaaagtga aggagagttt ctaatgaagt tcaaatcatt
      721 cttgttaacg gcagttcttg cgtttgtatc atatacatcc gtaagtgctc aagctacgac
      781 accggcacca gcaaaacagc ctgctcccgc tggaggcaat aatgctcctg caggcggcaa
      841 gctgggcgat gcactcgaaa gagtgacatt ggaagatttc gaaatggctg aagattggcg
      901 tgcaaaatcc acaacaccgc tcggagagac taaaactctg aagatgatcc agagaggact
      961 cattaaagat gtatttgatg aaaaaagcat gccagataca aaggatgaca aaggtaatta
     1021 taatccagct actgacaatc aggatgaaaa aatcggaaaa aatcatgttc tgggagtgaa
     1081 aacttacttt gctaacagag gatttgacag agtggaagtt gctcctcctc aagaattcat
     1141 catcaaaggg aaagctcgcc aagtatctat ttgggtattg ggcagaaaat accgtcacac
     1201 tctttatgca aaatttagag attacagagg aaatactcac aatattcgtt tgggcagact
     1261 cgatttcttt ggatggagac gtcttactgc tccgattcct ggttttattc cacaaagtgc
     1321 aagatactcc cttcttgaca aaaacatcag attcgtttca ttatttgtaa caagcgatgt
     1381 tcatgaagtc ggcggagaat tttactttta tgtagatgat ctggaagtca gaacagacaa
     1441 gcaggaatct gtttatcctg gttttgaaat caaagacaac tggtagaaat tatttggtat
     1501 aggggtacaa aaatgaacaa gaaaaaagca attttagctg gaatcgcttc ggttgggatc
     1561 acacttgccg gagttgcagg ttttgctttc gcacagcaaa agggagtgaa aacctccaat
     1621 ggtaatgatc tctcacaagg tgagctgaga gcaattactt tggaaaactg ggataaaact
     1681 gatctttggc aggtatttac tgataaagac agtatgactt acccagacaa tttggaacaa
     1741 gccaaatatg atccaaaact ggctcctaca tcaatggcga tgagagaagt gaagtatttg
     1801 gatggtgcat acccacgcga cgtaagatat ttggatacca gaaaagatga agagaaaaag
     1861 aacctgaaag ttcttggagt caaatttcag tttacatact ctggaaacaa tgaagtgact
     1921 attcgtccgc ctagaacacc tgattaccaa gtaaagagat acagaacgtt cctgactgga
     1981 aatgaggcaa atttagacag aaatgaagct aaaaagtctc cagaagacag aaaagttctc
     2041 gatctggaat acaaaattta cggtatcgat cttcctagtg tagctaaggc actttccgtt
     2101 tgggtttgcg gccgtggaaa tgattatgat ctggaaggct ggattgaaga ttacaaaggt
     2161 gatactcaca ttgtaaaatt cggttctgtc ggatttgtag gatggagacc aatgactgca
     2221 aaaatcccag tctctatacc acagttttca agcaactacc ctgcaaccag aacagctgta
     2281 tttaagcagt ttaaactccg ttccaattac agatcctcag gtgatccagt gattcttttc
     2341 tttgatgaat tcagagtact tactgacaca tttgaagtgc attttgatgg agcaaacctg
     2401 gattttgatg atgaagattg tgctaacaaa gctaaattgg atgcaatgat cgacaagaga
     2461 actcctggtg atccatatcg tacagtcaag tgcggcgata aaggtggagc tcctgcaaaa
     2521 ggttctgctc ctgcagcgcc tacaaaacaa taattttttt ctccttcaac aattgctcgg
     2581 gtgcacaccc gagctttttc tttccttttt ttctgcttta ctaattcact gatcctaaaa
     2641 atctagttct tataacgcac tatttctttt aaataattat tattcaatgg ggttgttcat
     2701 gtcacaagat actgccgtat taccagataa ggccagcgga gaatttcaaa aattaactgc
     2761 tctaataaat gaagaaatct atgtaagagt ggatgcaggc aatgttccgg taacaaaatt
     2821 taaaatttat gatgatctga tccagcacta taagcagctg ggaaagctga ctgaagcaaa
     2881 tcagctgatg aaagaacatc taaatgatca ccaagacagc atatcttcac gatatatgat
     2941 gggaatcatt tctcttatgc agaataagct ggaggattcc aatcatctaa aaacacttct
     3001 tgagcagctg aaaggtcatg gaaaatggtc gatcattgaa cacgtcgcag accagatttt
     3061 actttttgga gagcagagaa tggctctcaa atacaaagct gaggctttgg aaaaacagaa
     3121 taaaaataaa gaactgaagt tcgtactgga aaaactggct aaacacgaca gaaagaatcc
     3181 tgaaattgc
//
LOCUS       DAOQGJ010000210         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17856_length_3184_cov_3.077021, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000210 DAOQGJ010000000
VERSION     DAOQGJ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17856_length_3184_cov_3.077021"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..195
                     /locus_tag="PK683_04505"
     CDS             37..195
                     /locus_tag="PK683_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07740.1"
                     /translation="MKNKEKVSFGTLEDVYGAGYIADCSVMLDMDDFMFSIAFSSRKT
                     KDEVSDKL"
     gene            complement(223..420)
                     /locus_tag="PK683_04510"
     CDS             complement(223..420)
                     /locus_tag="PK683_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07741.1"
                     /translation="MGYIVWHNLEENRTWGLFLSDHGCRPLSEIRAKSYRFGVTFEEK
                     PVTAEPYRKCWICDAGIQYCR"
     gene            complement(479..2593)
                     /locus_tag="PK683_04515"
     CDS             complement(479..2593)
                     /locus_tag="PK683_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07742.1"
                     /translation="MIVFRFLLTFLILSLFSGLSAQERRSPKAEKGVIDLRDWNFEKD
                     GYVLLDGQWEFFWKKFISSRNEHFNSERNGFLEIPGLWNGFHVEGTEISGWGFGSYRL
                     KILLPENSPPLSIHFEGAATSAVLWINEAKLLENGVIGTEKSLTVPMTKPIVEEIGQH
                     FDELNAVLEVSNFSHYKGGAWESIRLGTKYQLQSYQRTKEETDIFLTGTILIMTLYHF
                     GLFSLRKKDRSSLYFALFCIAILFRIFTAGRLMYVKFPWIYWEFGNKIEYLSLYLCVP
                     AFFLFLKSLYPDEVSSRVGIFTWAVNGSMSAVVIAAPAHIYTRTLIPVEIALILTAIY
                     GTFCLGLAIWRKREGAGIALIGYLALILAGINDILYSQLIINTGYYTPVGLFFFIFSQ
                     SYMLSQKFSKAFYSVEVLSENLQKTNESYSRFVPIEFLHLLGKEKITDIQLGDQMQRE
                     MTVLFSDIRSFTSLSEKMTPKDNFDFLNSYLQRMSPVIRRNGGFIDKFIGDGIMALFP
                     DYVDDALKAAVEMHSELRDLNDKRMKKNYPVIRIGIGIHTGNLMLGTIGEKERMDGSV
                     ISDSVNIASRIEGLTKLYGADLLISETAFQKVKDISSFPCRKIQKVKIRGKENYITIL
                     EIFTDRFLKETEQKMLTADIFEKAMDFYTGRQFSEAVQLFEKVLKENPSDMTADFFLK
                     RSQKLQKFKGTGTLSEEEIDIL"
     gene            2925..>3184
                     /locus_tag="PK683_04520"
     CDS             2925..>3184
                     /locus_tag="PK683_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07743.1"
                     /translation="MSISTFQEPIQKKTSFRGSGVSSILIPADLMKRFQNCQKRYGGK
                     RQYFNHLILRYRVILRTFANDSNGMTTEYQFKNQSLKKSKLPS"
BASE COUNT          994 a          693 c          609 g          888 t
ORIGIN      
        1 tgacagactg atacttggaa ttgaaatctg ctgactgtga agaacaagga aaaagtatcc
       61 ttcggaacac tggaagatgt ttacggagcc ggatatattg cagactgttc agtaatgttg
      121 gatatggacg attttatgtt ttccattgcg ttcagcagca ggaaaaccaa ggatgaagtg
      181 tccgataaac tatgaagcaa aaatggaatc gtaaccaaaa gatcatctgc aatactgaat
      241 acctgcatca cagatccagc atttcctgta aggttctgcg gtaacgggtt tttcttcgaa
      301 tgtcaccccg aatctgtagg atttagccct gatttcagaa agaggtcggc atccgtgatc
      361 tgagaggaac agaccccagg ttctgttctc ttccagattg tgccatacaa tatatcccat
      421 tgtcgcagtc cagaataaac agaaaattat gacaaatttt accatggtct gaacctcctt
      481 acagaatatc tatctcttct tcggagagtg ttccagtacc tttgaatttc tgaagtttct
      541 gagatctctt cagaaaaaag tcagctgtca tatctgaagg attttcctta agaactttct
      601 caaaaagctg cactgcctca gaaaactgcc ttcctgtata aaagtccatc gccttctcaa
      661 aaatatcagc agtgagcatc ttttgctccg tctctttcag gaacctgtcg gtaaatattt
      721 ccaatatagt gatatagttt tcctttccgc gtattttaac cttctgaatc tttctgcagg
      781 gaaaagaaga gatgtctttg accttctgaa atgctgtttc gctgatgaga agatctgctc
      841 cgtaaagttt tgtaagcccc tctatcctgg atgcaatatt gacagagtct gaaataacgc
      901 tcccatccat tctttccttc tcgccaatgg ttcccagcat tagatttcct gtatgaatcc
      961 cgattccgat cctgattaca ggataatttt ttttcattct tttatcattc agatctctaa
     1021 gttcagaatg catttccaca gcggccttca gtgcatcatc cacatagtcc gggaaaagag
     1081 ccatgattcc gtctccgatg aacttatcga tgaaaccccc gttccttctg attacaggag
     1141 acattctctg cagataggaa ttcagaaaat caaaattgtc ctttggagtc attttttcag
     1201 atagagatgt gaatgaacga atatcggaaa agagcacagt catttctctc tgcatctgat
     1261 ctcccagctg gatgtcagta attttttcct ttcccagaag atgcaggaat tcaatcggaa
     1321 caaaacggct gtacgattca tttgtcttct gaagattttc ggaaagaacc tccacgctgt
     1381 aaaatgcctt ggaaaacttc tgagaaagca tataggactg agaaaaaatg aaaaagaaaa
     1441 gacctacagg agtataataa cctgtattaa taataagctg agaatataat atatcgttaa
     1501 tcccggctaa aatcagggcc agatatccta tcagggcaat tcctgctcct tccctttttc
     1561 tccagatggc cagaccaagg cagaaagtgc cgtagatcgc ggtaagaatg agagcaattt
     1621 ccaccggtat cagtgttcgg gtgtagatat gagcaggagc tgctataaca actgcactca
     1681 tcgaaccgtt caccgcccag gtaaaaattc cgaccctgct gctgacctca tcaggataaa
     1741 gtgattttag gaacagaaag aatgcaggaa cacagaggta aagactcaga tattcaatct
     1801 tgttgccgaa ctcccagtaa atccagggaa acttcacgta cataagtctt ccggcagtga
     1861 atatcctgaa aagaattgca atacagaata atgcaaaata cagactggaa cggtctttct
     1921 ttcggagaga aaaaagacca aaatgataca aagtcataat caggatcgtg ccagtcagaa
     1981 atatatccgt ctcttccttc gttctctgat aactctgcag ctgatatttc gtgcccagcc
     2041 tgatgctttc ccaggcacct cccttatagt gcgaaaaatt ggatacttcc agcacagcat
     2101 tgagttcatc aaaatgctga ccgatttcct ccacaatcgg ttttgtcatc ggaacagtga
     2161 ggcttttttc agtcccgatg acgccgtttt cgagaagctt agcttcgttg atccataaaa
     2221 ctgctgaagt agccgccccc tcaaaatgga tggaaagagg aggtgaattt tcaggaagaa
     2281 gaatcttgag tctgtaactg ccgaaacccc agcccgaaat ttcagtccct tccacatgaa
     2341 acccgttcca aagaccggga atctccagaa atccgtttct ctccgaatta aaatgttcgt
     2401 ttctggaaga aataaatttt ttccagaaaa attcccactg cccgtccagg agaacatatc
     2461 cgtctttttc aaaattccag tcccgaagat caatgactcc cttttcagcc ttaggactgc
     2521 gcctctcctg cgcagacaag ccggaaaata aagacagaat gagaaaggtc agcagaaatc
     2581 tgaatacaat cataacagcg tcagtttaca gacctgaaat aaaattcaaa cctttaattt
     2641 gtttttcaat cggatgaatt tcagaggatt catttctgcc gatgaaaaaa gaactcaagg
     2701 ctcatcattt aaatatattc cgcaaccttc tccaatagca gttcctacct agaaaatcct
     2761 ttgttgacaa gtttgcatat tttacgctga gtcacctttt tattacgtag gaactgctcc
     2821 agaacatttt cagagaattg caccctttca gtaaaaatct gttcataagt gacgtatgat
     2881 gaaaaaaaaa tgcggatctg atcaaaaaaa aacaggtata taatatgagc atttcaacat
     2941 ttcaggaacc tatacaaaag aaaacttcat ttagagggag cggagtatcc agcattctga
     3001 ttccagcgga tttgatgaaa cgcttccaga attgtcaaaa aaggtatggc ggtaagcggc
     3061 agtattttaa tcacctaatc ctccgctata gggtaatttt gagaacattt gcaaatgatt
     3121 caaatggaat gacaacagag taccagttca aaaatcagag tctaaaaaaa agtaagcttc
     3181 cgtc
//
LOCUS       DAOQGJ010000211         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17859_length_3184_cov_2.757430, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000211 DAOQGJ010000000
VERSION     DAOQGJ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17859_length_3184_cov_2.757430"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2436
                     /locus_tag="PK683_04525"
     CDS             <1..2436
                     /locus_tag="PK683_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07744.1"
                     /translation="SKSGDLILTSSFVNPAGAFTQIKKIVFNKMGNVDSSGNPNSNAK
                     TVCEDSCTSLLSPINLSTDSFMTGSMALTEGGNTYYFDITTAQNSRIQRYFSFNYGNV
                     NSNPSALINNVASGVLDESQMMNVLKRMIETFIKGKFRVSDAGTPKTFNQFASQPMSS
                     SKDTSKCINYGSFSFITSYGDNPSGGYCGGNGENPGGFQAVTGAGCFGNATLDMNVYV
                     TGVSIPGFTGGVPTVEAALGVNSNGEVGIDLKGRKAYLNLAIIAKNRDSMLCLVGSGN
                     KFHFSTSVELNAGNSILRLARSRNTMSVDSSGKIVINVKTPYTPNDALGANFYVSEWV
                     NNLSVAGVSLVDSTSWVSNLLSFISEAIGNGVVPQVQPGITQSVLKDMIQKITPNVLN
                     AIVGNLMNPGVDIKLPDYLPAPLANYPLNAKIQLSTDASVRSDGTNKGIVASVHAAIT
                     SKNPLAPASQRGHANGTLCGASGCMVYTKNPAVPLINPSTSAPFAMSAANPGFLLALH
                     SDAVTQAAYHLWKNRAIDINVDSAFISTVNSYAGSDPLLKLTDSLLRASAIMSIIAPG
                     QSNLQGIDNSNNLLPAICQDDQVRFKIDPVMPPVAKMIDNSGFDATVNTPNLNVTFSD
                     LQVTIEGKRTDNSAACIAQRGTATNAYYTMASLRVSMNANASMRFTEFDNPNTVGHDR
                     LNALSLRIFTDGMKYSIEVLEGQVYNPYGLDPNGIKSVFNPMVKSLVVPLVNSILNSL
                     PLPAQLDFNALTYPAAGTACKISTKTDNSIMFVQKTVPAVDASANPYLLAQATLMGAG
                     LADPETMLEANCR"
     gene            complement(2569..>3184)
                     /locus_tag="PK683_04530"
     CDS             complement(2569..>3184)
                     /locus_tag="PK683_04530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH07745.1"
                     /translation="DFQKFKVKPIFRKYRISETSKNRYEKTLIFTGRYSPNKKLEDCI
                     YSIFFLRKIDPDFRFIFLGKRIPVFQKYSDFLHRISKELNLLNSVYFIESASDDFILK
                     KMTGSSVYLSCSEHEGFGIPVIEAFGAGLPVIYSSIPALKEITKGAGAAVNKKRFELI
                     AELIFEMHSNSEFRERTVHRQNIVAEYYNSLYESLEKSAESIFG"
BASE COUNT          978 a          695 c          657 g          854 t
ORIGIN      
        1 agcaagtctg gagatttgat actgacatcc tcctttgtca accctgccgg tgcatttacc
       61 cagattaaaa aaattgtatt caataaaatg ggaaatgtag attcctccgg aaatccaaac
      121 tcaaatgcaa agacagtgtg cgaagacagc tgcacgagtc ttctgtcgcc gatcaatttg
      181 agcacagatt cctttatgac aggaagtatg gcactgaccg aaggcggaaa tacttactac
      241 tttgacatta ctactgctca gaacagcaga atccaaagat atttcagttt taattatgga
      301 aatgtcaatt caaacccaag cgcactgatc aacaacgttg catccggagt gctggatgaa
      361 tcgcagatga tgaatgttct gaaaagaatg atagaaacct ttataaaagg aaaattcaga
      421 gtatcggatg caggaactcc taaaacattc aatcagtttg cttcacagcc gatgtcttct
      481 tcaaaggata catccaaatg catcaattac ggaagttttt cattcattac aagttacgga
      541 gacaacccaa gcggcggtta ctgcggcgga aacggtgaaa atccaggcgg gtttcaggct
      601 gtcaccggag caggatgttt tggaaatgca actttggata tgaacgttta tgtaaccgga
      661 gtcagtattc caggttttac aggaggagtt cctacggttg aggctgcact gggagtcaat
      721 tcaaacgggg aagtgggaat tgatctgaaa ggaagaaagg catatcttaa cctcgcaatc
      781 atagcaaaaa acagagacag tatgctgtgt ctggtcggat caggaaacaa atttcacttc
      841 tccacttctg ttgaactgaa tgcaggaaat tcaattctga gacttgcacg ctccaggaat
      901 acaatgtcag tggattcaag cggaaaaatt gtaatcaatg tcaaaacccc ttatactccg
      961 aatgatgctc tgggagcaaa cttttacgta tcagagtggg tgaacaactt gagtgtcgct
     1021 ggggtcagcc tggttgattc tacaagctgg gtcagcaatc tgctttcgtt tatttccgaa
     1081 gccatcggaa acggtgtcgt tcctcaggtt cagcctggca tcacgcagtc tgtgctcaaa
     1141 gatatgattc agaaaatcac gcctaacgta ttgaatgcaa tagtaggaaa ccttatgaat
     1201 cctggagtag atattaaact tccagattat ctccccgctc cacttgcaaa ctatcccctc
     1261 aatgcaaaaa ttcagctttc tactgacgcg tcggtcagga gcgacggaac aaacaaaggt
     1321 atagttgcct ctgttcatgc tgcaatcact tccaaaaatc cccttgctcc tgcaagccag
     1381 agaggacatg ccaatggaac actctgcggc gcctcaggat gtatggtata tacaaaaaat
     1441 cctgctgtac ctcttattaa tccttcaaca agtgcaccat ttgcaatgag tgcagcaaat
     1501 ccggggttcc tgctggcact tcattctgac gcagtcacac aggcagccta tcatctttgg
     1561 aaaaacagag ccatcgacat caatgtagac agtgccttca tcagtacagt gaactcgtat
     1621 gctggaagcg atccacttct gaagctgaca gattccctgc tcagggcatc tgcgattatg
     1681 tcaatcatag cacccggcca gagcaatctg caaggaattg acaattccaa taatcttctt
     1741 cctgcgatct gccaggatga tcaggtcaga tttaaaattg atccggttat gcccccagta
     1801 gccaaaatga tcgacaacag cggttttgat gcaacagtaa acactccaaa tctgaatgtc
     1861 acattcagcg atctgcaggt gaccattgaa ggaaagagaa cagacaacag tgccgcctgc
     1921 attgcacaga gaggcacagc aacaaatgca tattatacaa tggcctccct gagagtcagt
     1981 atgaatgcaa atgcctccat gagatttact gaatttgaca atcctaacac agtcggtcat
     2041 gacagactca atgcactgag tcttcgaata tttactgacg ggatgaagta ttccattgag
     2101 gtcttggaag gacaggttta caatccttac ggactggatc cgaacggaat caaatctgta
     2161 ttcaatccga tggtgaaatc tcttgtagtg cctctggtaa acagcatact gaacagtctc
     2221 ccgcttcctg ctcagctgga cttcaatgca ctgacttatc cggcagcagg cacagcctgc
     2281 aaaatcagca ctaaaacaga caattccatt atgtttgtgc agaagacagt gccggcagtg
     2341 gatgcatctg caaatcctta cctgcttgca caggcaacct tgatgggtgc aggtcttgca
     2401 gatccggaaa caatgctcga agctaactgc cgttagaccg caaaagccct gcaggagttt
     2461 ttaccttcag ggcctttctg tacttattgc ataaagctca agctgaattc gaaaaattaa
     2521 tgaagacaat caaaatcaga aactgtaaaa acgatctgtc cctggaagtt atccaaaaat
     2581 agattcagct gatttttcca gggattcata aagtgaatta taatattcgg caactatatt
     2641 ctgccgatga acagtcctct ctctgaattc agaatttgaa tgcatttcaa aaatgagttc
     2701 cgcgatgagt tcaaatcttt ttttatttac tgcagcacct gccccttttg taatttcttt
     2761 taatgcagga atggaactgt atatgaccgg aaggcctgca ccgaatgcct cgataaccgg
     2821 aattccgaaa ccttcatgtt cgctgcagga gagatacact gaagatcctg tcattttttt
     2881 taatataaaa tcatctgaag cagattcaat aaaataaacg gaattcagaa gattcaattc
     2941 ttttgaaatc ctgtgaagaa aatcagaata cttctgaaat actggaattc ttttgcctaa
     3001 aaaaataaat ctgaaatcag gatcaatttt cctcaaaaag aatatcgagt atatgcagtc
     3061 ttcaagtttt ttatttggag aatatctgcc ggtaaaaatt aaagtttttt catatctgtt
     3121 tttactcgtc tccgatattc tgtatttccg gaatatcggc ttgaccttga atttctgaaa
     3181 atct
//
LOCUS       DAOQGJ010000212         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17864_length_3183_cov_4.341432, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000212 DAOQGJ010000000
VERSION     DAOQGJ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17864_length_3183_cov_4.341432"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1497)
                     /locus_tag="PK683_04535"
     CDS             complement(145..1497)
                     /locus_tag="PK683_04535"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNH07746.1"
                     /translation="MLLKAKKFCKKIFRYAGVTGQRLTGSAGLFLLKDYMVKTGLPER
                     FSAGITDMREQFRIRYSASELHLCGVLRILNGQFRHSHSRNTDVSLFETMYGSGTVPD
                     FRTLIYYHERNPHLWKEQEKILYDMCTENIRKRMPDVKNPRITLDIDQTAKAVYGRQA
                     GARKGYSAKERNSKLFQCAVWTVRETKTVLKIELLPGETHSGYDLLERLRPTLEKLKK
                     DGFRLRLVLDSGYENTAVFDYLESEGIEFICAAKQRKAVKLRGRNAKNRTLLKEKGNV
                     TAFIKERKLNTPEGSEFRQIYVQNRIACDEHGQLYFKEFDSGEFTNVFVTNMKIARKN
                     IYKIYRKHASVETVIEELKNDFGLAASHSMFFPVNCAMAQTCAIAYNVKNMFVAEYSV
                     LRNKKETVKLSTFQRKFINIPGIIANRSGRTVLRLEHSAFQKLKGYFSIFGYNINAFS
                     "
     gene            1995..2558
                     /locus_tag="PK683_04540"
     CDS             1995..2558
                     /locus_tag="PK683_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH07747.1"
                     /translation="MQSLIVNLGKYMELSPSLQKAISERLVFQKVKRRKALAESGKVC
                     ENLFYVQSGLIRGYYYHKRKDITNWFSVENEFATSMYSFVSGKPNMENIETLEDSELY
                     VMKRKDQLYLYENFPEMNLLGRLLMEKYYLELGERAVSLQFQTAKERLGAFQQKRPDL
                     FDRVKLGQIASYLGITIETLSRIRSAS"
     gene            complement(2555..>3183)
                     /locus_tag="PK683_04545"
     CDS             complement(2555..>3183)
                     /locus_tag="PK683_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07748.1"
                     /translation="RFLTIGKYLLLEAEEMYNIKDAERNDFDYRIGRVRHAILDRIAD
                     DLRPVNYDYKADAIHKLRNIFALVEMLSIGYPDPKLPKLNSQQLEHYKKESIKAFDFI
                     VMKRDYILSYPSPERLYEWMDRYESYVFGTKPRALGGVPSHLSRKAYITLANHFPLSE
                     YFFDYKKKKKETVNAMLTRLRGDLQKMLDSSQKLSQPLFKPYDVGGDI"
BASE COUNT          827 a          692 c          652 g         1012 t
ORIGIN      
        1 tgttaacaca attgccgttt ttgatgaagt atcaataacc gatttgaagg aatacgagat
       61 tacggatatc tatggaaaag atatagtgtt ttctcaaatt aagcataata taactcagat
      121 ttctaattag atcctgtaaa aagtctacga aaaagcattt atattgtatc caaaaatgga
      181 aaaataacct ttgagcttct ggaacgcaga atgctccagc ctgaggactg tccttccgga
      241 tctgtttgcg atgatgcccg gtatgtttat gaattttctc tgaaatgtcg aaagcttcac
      301 tgtttctttt ttgtttctca gaacggaata ttcagcaaca aacatatttt ttacattata
      361 agctattgca cacgtctgtg ccattgcgca gtttacagga aagaacatgc tgtgggatgc
      421 cgcaagtccg aaatcattct tcagctcttc aatcacagtt tccacagatg catgttttct
      481 gtatatttta taaatattct ttctggcgat cttcatattt gtcacgaaga cattggtgaa
      541 ttctcctgag tcaaactcct tgaaatacag ctgcccgtgc tcatcgcagg cgattctgtt
      601 ctgaacatag atctgccgga actctgaacc ttcaggcgtg ttcagttttc tctcttttat
      661 gaatgcagtc acgttcccct tctccttcag gagagttctg ttctttgcat ttcttcctcg
      721 aagcttcacc gcctttctct gctttgcggc acagatgaat tcaatgcctt ccgattccag
      781 ataatcaaag accgcagtgt tttcatagcc ggaatcaagc acaagtctca ggcggaatcc
      841 gtccttcttc agtttttcaa gagtgggacg cagtctttcg agcaggtcgt atccgctgtg
      901 agtctctccg ggaagaagct caatcttcag gacagtcttc gtttcccgga cagtccatac
      961 cgcacactga aacagtttcg agttcctttc ctttgctgaa tatcctttcc gggctcctgc
     1021 ctgacggccg tatactgcct ttgctgtctg atcaatatcc agtgtgatgc ggggattttt
     1081 cacatccggc attcttttcc ggatattttc agtgcacata tcatacagta tcttttcctg
     1141 ctctttccat aaatgcggat ttctttcatg ataatatatc agcgtccgaa agtccggaac
     1201 tgttccgctt ccgtacattg tctcaaacag actgacgtcc gtgttccggc tgtgagaatg
     1261 tctgaactgt ccgttcagta ttctcagcac tccgcacagg tgaagttcag atgcagaata
     1321 tctgatcctg aactgttctc tcatatccgt tatcccggcg ctgaatctct ccggcagacc
     1381 tgtctttacc atatagtctt tcagcagaaa cagaccggca ctgcctgtaa gcctctgtcc
     1441 ggtaactccc gcatatcgaa aaatcttctt gcagaatttc tttgctttta ataacattca
     1501 gttagccctt tgtcctggtt gggattggcg ttttaatcag gttttcaaag ggttttgttt
     1561 gtacagctct ttatttcatt ggaaatctgg gtattactgt gtttaccggc aatgcagcaa
     1621 attcaagctt tccgttctta tgaaaaaata cccattctcc gggtttacag ggaattccct
     1681 ggattatttc gttttttcgg cattctctgt cctctcccaa aatgcctgca gcgcatactg
     1741 taaatacaga tattattttg atgcggcagc tcatatatcg tcttccttat tcagtatttt
     1801 cattcttttg caagagtaac tgtccaaggg aaacaagtct gacatcggaa cagttttttt
     1861 gtgataaatt atgattcaat ttttggcaga ctcaacatct gcctggatgc tgtgacgcaa
     1921 gttcttaaaa cagatccctt cagagctcag gaaaatgatt ttccttttgt gcctgctgaa
     1981 taaaaaggac atttatgcaa agtctgattg tcaacctggg aaagtacatg gagctcagtc
     2041 cttctcttca gaaggcgatt tcagaaagac tggtatttca gaaagtgaaa cgaagaaagg
     2101 ctctggcaga atcaggcaag gtctgcgaga atctttttta tgtacagtcc ggtctcatca
     2161 ggggatacta ttaccataag agaaaggata tcacaaactg gttttcagtg gaaaatgaat
     2221 ttgccacttc catgtactcc tttgtttcgg gaaaacccaa tatggaaaat attgagacac
     2281 tggaagattc agagctgtat gtaatgaaaa gaaaagatca gctttatctg tatgaaaatt
     2341 tccctgagat gaatctgctt ggaagacttc ttatggaaaa atactatctc gaactcgggg
     2401 aaagggcagt gtcgcttcag tttcagacag caaaggaaag acttggcgca tttcagcaga
     2461 aaagacctga cctgtttgac agggtcaaac tgggtcagat cgcttcttat ctgggaatca
     2521 caatcgaaac tctgagcaga atcagatctg cttcttagat gtcgccgccg acatcgtaag
     2581 gcttgaagag gggctgagaa agcttctgtg aactgtcaag catcttctgc agatcacctc
     2641 tcagtcttgt gagcattgca ttgactgttt cctttttctt tttcttgtag tcaaagaaat
     2701 actctgaaag aggaaaatga tttgcaagag tgatgtatgc ctttctcgaa agatgtgaag
     2761 gaactccgcc cagtgcgcgc ggttttgtcc cgaatacata agattcatat ctgtccatcc
     2821 actcatacag acgttcaggg gaagggtagg agaggatata gtctcttttc attacgatga
     2881 agtcgaatgc cttgatcgat tctttcttgt aatgttcgag ctgctgactg ttgagtttag
     2941 gcagtttcgg gtcagggtat ccgatcgaaa gcatttccac aagcgcaaat atgtttctga
     3001 gcttgtgaat tgcgtcagcc ttgtagtcat aatttaccgg tctcaggtca tctgctattc
     3061 tgtcgagaat cgcatgtctg actcttccta tccgataatc aaaatcattt ctttctgcat
     3121 ccttaatatt gtacatttcc tctgcttcca gaagcagata tttgcctatt gtaaggaaac
     3181 gtc
//
LOCUS       DAOQGJ010000213         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17865_length_3183_cov_4.204284, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000213 DAOQGJ010000000
VERSION     DAOQGJ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17865_length_3183_cov_4.204284"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PK683_04550"
     CDS             <1..541
                     /locus_tag="PK683_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07749.1"
                     /translation="FAPYASEVKVLEHKRETEKYEGKQGYWIRLAFSEKEGWAFSTDL
                     SDEKQKTVLEFPGDYPRHWIIAHFTDGKYRLTEWCGKFEEIILEEKSGVLKFIHYKID
                     SSDVYTIKDRQNSAAGITLFGEVQDSALGTASSFEVLFNYDRRTGVGEWRFSDGGSWD
                     TVEKSKIENYTIQKITDCR"
     gene            544..1041
                     /locus_tag="PK683_04555"
     CDS             544..1041
                     /locus_tag="PK683_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07750.1"
                     /translation="MYIKLFFLLILIPFIYCKKEDKKLYIKEKREMKINLTDQSFLLP
                     DDKLKTVLKDREWTEVTQDEEAFVTLNCGTPPRKVFFDGKFIVWKKEESSEKFEILGY
                     KQENEILILKTGREKSGELYPVSYAYVSEYEISDWTFHDETSQYASKDKEKTLSRQKK
                     ETCKE"
     gene            1078..2319
                     /locus_tag="PK683_04560"
     CDS             1078..2319
                     /locus_tag="PK683_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07751.1"
                     /translation="MTEKIYGSKEHSYNSLNRLILLLSFFISGCSASSSSESANVNSG
                     SAPFDYKSKFSSQIFQVGPADRNRFFDCVEKEPVTYPIPFWVINTEFNYFGKALPGLQ
                     YGLIEKDKWPILVGMYGPPESVKKPKAVIVMRGDTSGYHKSAAAKPYVYYMQKYGKNE
                     VMFFLDEFYYLNKNSRNLESSPIMSLCHNRYYWFGYVGRAWPPLEKGGNWEILDKFFP
                     EDQEYDIFSYSNATWPRMEFILRENAPGYTPVFKKIKDPKAFLADYIQNTRQKSPRTN
                     KIMKMLDIDGNYHSGKPMWDLLAYLRETVDKDPNKYYHSVVRIDKEDAYPIQVKMVQA
                     LDLQGEEDATGIVRFSNERKNIIIDFMTTPWAPHYINMGIDLRRATVFKQRDPSSIRR
                     KWLNHYNMIRNAVDIYAAGGK"
     gene            2326..>3183
                     /locus_tag="PK683_04565"
     CDS             2326..>3183
                     /locus_tag="PK683_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNH07752.1"
                     /translation="MKAEHLQSTGVQIRGFGHYFPEKILSDKEIEDSLEFKDVHPTEK
                     TVMGDINVHQRHRSSEKETSIYMAAEAGRMALKDSGISPDSIDLLIVGNWTERWLLPD
                     LAVTVAVQLGLKNALSFDLGGACAGFIHGVHTAMMYLGSGKYKRALIIGSERFSIRTR
                     PRSRGEFITGDAAGAVVLEQTDDRKYGIIDTHFHSDGNLAYLITTPFPNGWIKSHPDL
                     PKNAIEKSVEAINGLLERNSLKIEDIDWVAPHPGTDVVLKGVTTGAKFQTEQYLANFH
                     YRGNTSSASV"
BASE COUNT         1038 a          616 c          724 g          805 t
ORIGIN      
        1 ttttgctccc tatgcatctg aggtcaaggt tcttgaacac aaaagggaaa ctgaaaaata
       61 tgagggaaag cagggatact ggatacgtct ggcattcagt gaaaaggaag gctgggcatt
      121 cagcactgat ctttccgatg aaaaacagaa aactgttctc gagtttccag gcgattatcc
      181 cagacactgg atcattgcac attttactga cgggaaatac agactgaccg aatggtgcgg
      241 aaaatttgaa gaaatcattc tggaagaaaa atccggtgtg ctgaaattca ttcattacaa
      301 gattgattcc tcagacgtgt atacaatcaa ggacagacag aattctgctg cgggaattac
      361 cctattcgga gaagtccagg acagtgctct tggaacagcg tcttcctttg aggttctttt
      421 taattacgac agaagaacag gcgtgggtga atggcgtttt tccgacggcg gaagctggga
      481 tactgtggaa aaaagtaaaa tcgaaaatta taccatacag aaaattactg actgcaggta
      541 agtatgtata taaaactttt ttttctgctt attctcattc cttttatcta ctgcaagaaa
      601 gaagataaaa agctttatat taaggaaaaa agggaaatga agatcaatct caccgatcag
      661 tcattccttc tgcctgacga taaacttaag acagtactga aagacaggga atggactgaa
      721 gtcactcagg atgaagaggc ttttgtgaca ttgaactgcg gaactcctcc aagaaaggtt
      781 ttttttgacg gcaaattcat tgtatggaaa aaagaagagt cttcagagaa gtttgagata
      841 ctgggctaca agcaggaaaa cgaaatcctg atactgaaga caggcagaga aaaaagcgga
      901 gaactttatc ctgtgagtta tgcctacgtc tctgaatatg aaatatcaga ctggactttt
      961 cacgatgaaa cttctcagta tgcatcgaag gacaaagaga aaactttaag cagacagaaa
     1021 aaagaaactt gcaaggaatg agtgcagtct aaggattact ccatcagagc ttaaacaatg
     1081 acagaaaaaa tatatgggtc gaaagaacat tcctacaatt cactgaacag actgattctg
     1141 cttttatcct tcttcatttc aggatgcagt gcctcctctt cttcggaatc tgcaaatgtc
     1201 aattctggaa gtgctccctt tgattataag tcaaagttca gcagccaaat ttttcaagtt
     1261 ggtccggcag acagaaacag attctttgac tgtgtggaaa aggaacctgt gacgtatccg
     1321 attccattct gggtcatcaa tactgaattc aattatttcg gaaaggcact gccggggctt
     1381 cagtacggtc tgattgaaaa agacaaatgg cctattctgg tcggtatgta cggtcctcct
     1441 gaatcagtca aaaagccgaa agcagtcatt gtaatgagag gcgatacaag cgggtaccat
     1501 aagtccgcag cagccaaacc ttatgtttat tatatgcaga agtacggaaa gaatgaagtt
     1561 atgttctttc tcgatgagtt ctattatctg aacaaaaact ccaggaacct ggaatcatca
     1621 ccgatcatgt ctctctgcca taacaggtac tattggttcg gatatgtagg aagagcctgg
     1681 ccccctctcg aaaaaggcgg gaactgggag atactggata agtttttccc ggaagaccag
     1741 gaatatgata tcttctccta cagcaatgct acatggccca gaatggaatt tatcctgaga
     1801 gaaaatgctc caggttatac ccctgtattc aaaaagatca aagacccgaa agcatttctt
     1861 gcagattaca ttcagaatac aagacagaaa agtccgcgta caaacaagat catgaaaatg
     1921 ctcgacattg acggcaacta tcattcggga aagccgatgt gggatctgct cgcatatctc
     1981 cgtgaaacag tggacaagga tcctaacaaa tactaccatt cagtagtcag aatcgacaag
     2041 gaagatgcct accctattca ggtaaaaatg gtgcaggcac tggatctgca gggtgaagaa
     2101 gatgcaacag gaattgtgcg tttctcaaat gaaagaaaaa atattatcat agattttatg
     2161 actactccct gggctcccca ttatataaat atggggattg atctgagaag agcaaccgta
     2221 ttcaagcaga gggatccttc gagcatacgg aggaaatggc tgaatcatta taatatgatc
     2281 agaaatgcag tcgatatcta tgcggccgga ggaaagtgag gggatatgaa agcagaacat
     2341 ttgcagagta caggagttca gatcagagga ttcggtcatt attttccgga gaaaattctc
     2401 tctgataagg aaattgaaga cagtctcgaa ttcaaggatg ttcatcctac agaaaaaact
     2461 gtaatgggtg atatcaatgt tcatcagaga cacagatcct ctgaaaagga gacctcaatt
     2521 tatatggcag ctgaagcagg ccggatggct ttgaaagatt cgggaatcag tcctgattca
     2581 atcgatctgc tgattgtagg aaactggaca gaaagatggc ttcttcccga tcttgcagtc
     2641 actgttgcag tccagcttgg actgaaaaat gctctgtctt ttgatctggg gggagcatgt
     2701 gcaggattca ttcacggagt ccacactgca atgatgtacc ttggatccgg aaaatacaag
     2761 cgtgcactta ttataggttc tgagagattt tccatccgca caagacccag aagcagagga
     2821 gaattcatca ccggtgatgc agccggagcg gttgtgctgg aacagacaga cgacagaaaa
     2881 tacggaatca tagacactca ttttcattct gacggaaacc ttgcctatct catcaccaca
     2941 ccgtttccaa acgggtggat caaaagtcat cctgatcttc ccaaaaatgc aattgaaaaa
     3001 tcagtggagg caatcaacgg ccttctggaa agaaacagtc tgaagattga agatattgat
     3061 tgggtggcac cgcatcctgg aacagatgtt gttctgaaag gagtgactac aggagcaaaa
     3121 tttcagacag aacagtatct tgcaaatttt cattacagag gaaacacatc ttccgcatct
     3181 gtt
//
LOCUS       DAOQGJ010000214         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17946_length_3174_cov_3.622635, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000214 DAOQGJ010000000
VERSION     DAOQGJ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17946_length_3174_cov_3.622635"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PK683_04570"
     CDS             complement(<1..1204)
                     /locus_tag="PK683_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07753.1"
                     /translation="MIEDTLWFIDKKKENCGICGAEESVRIEYYNAVRLPLDSNSEFI
                     TRNNVRKKTKKVCEKCSHTEESWEEWVETRYPKGYESDFLRRRALYFMEHVPSMAGEG
                     LDDLLKIRPYDLEMLYEYAKFLIRKKDLVEAYPILQRAAEKKKDFLPAVETLNDLLYS
                     LFIAQNEFWYEKRLYYGRFVKLFSSFIELRNRLNENLRYLLDYHKNNPAILSKYCLQT
                     GNLLEDKRNFSMALQYYEYVLNCSQELWQKSESCIRMAVVYSNLQDYKNAYIYLDKSY
                     SISPNVLVHLHRSRIYSKQGETEKASSELSYYLKQMDKLIESDPENPRLFYYKGLGLS
                     QIETPEKMAEFYSSVTEELTIDLDFRKIFYERINYYQSPPKESVTENPDISCPVCNFH
                     KLRHSENGN"
     gene            complement(1211..>3174)
                     /locus_tag="PK683_04575"
     CDS             complement(1211..>3174)
                     /locus_tag="PK683_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH07754.1"
                     /translation="VFAERTVRLSENTRKAEIGKKMNFLYQEGKDLNFTEIFSPEIQK
                     QFIPSVDTILQLGFKKDPVWIGFKVFEESEKQRDWVLSISFPILDHIEIYTQEGTEYK
                     KRTAGDSYPFYSREINHRNFIIPMNFAPGKETSVWLKIHSSSSITVPLTLYTKDELRT
                     DGMLTEGWHFLFYGVLTVMIIYNAFICIAFRSLTYLLYSLSTLLFLVYYLCFNGHGFQ
                     YLWPDSPKFQNYAVPVFVHFSWIFTAEFTLRFLHLKELSPALHRTVRMMEVITSLSLL
                     ALFHSIMTALILQSIFGAPSVLLLLFCGFKSWKRGNKSARFFVMAWGVFFCGVFMVLL
                     RSLGFLGMNLVTHYTMQAGTMIELVFLSLALADRYKFIQEENVRVQKTLLETQIRHTE
                     TLEEKVLQRTEELKSTLATIRQDLSLAGKIQQGLLNIHYSIMTKMDIVPLYIPISEVG
                     GDYYSINRLDEWRFRFFLADATGHGVQAALITMAVKGIYDNIREFHLGTADILSIFNN
                     EFTDKYSYLNALLTCIIIDIDLQNQRITYASAGHPAAVIVRKNSKDLLEKTGSMVGLT
                     KNVKFASKELEFTASDKIYAFTDGAFEQFNSSEEEFGEERLYAILMEYRTMSIEDTVR
                     AVRKNLYSFLGERERQDDITVIGIGYNKV"
BASE COUNT          863 a          659 c          653 g          999 t
ORIGIN      
        1 ggtttccgtt ttcagaatgt ctgagtttat ggaaattgca gacggggcag gaaatgtctg
       61 gattttctgt gactgattcc ttcggaggac tctgataata attgattcgt tcataaaaaa
      121 ttttacgaaa gtccagatca atcgtcagct cttcagtaac ggaactgtaa aactcagcca
      181 ttttctcagg agtttcaatc tgtgacagtc ctaatccttt ataataaaac agtctaggat
      241 tttcaggatc ggattcgatc agtttatcca tctgcttcag gtaataggaa agttctgaag
      301 atgctttttc tgtttcgccc tgcttgctgt aaattctgga cctgtgcaga tgtaccagta
      361 cattcggaga aatggaataa cttttgtcca aataaatata tgcattttta taatcctgaa
      421 gattggaata tacgacagcc atccggatgc agctttcgga tttctgccag agttcctgag
      481 aacagttgag tacatattca tagtactgaa gagccatgct gaagttcctt ttgtcttcga
      541 gaagatttcc tgtctgaagg cagtatttgg aaagaatcgc aggattgttt ttatgataat
      601 ccagaagata acgcagattc tcgttcagtc tgttcctaag ttcaatgaag ctggagaaca
      661 gttttacaaa ccgtccgtaa tagagtctct tttcatacca gaactcgttc tgggctatga
      721 atagagaata gagaagatcg ttcagtgtct caacagctgg aagaaaatcc ttctttttct
      781 ctgcagctct ctgcagtatc ggataggctt caaccagatc ctttttccga atcagaaatt
      841 ttgcatattc atagagcatt tccagatcat aaggtctgat tttcagcaga tcgtctaaac
      901 cctctccggc cattgacggg acgtgctcca taaagtaaag ggctcgtctt cttaaaaaat
      961 cggactcata tcctttagga tatctggtct caacccattc ttcccagctc tcttctgtat
     1021 gactgcattt ctcacagact ttttttgtct tttttcggac attgtttctg gttatgaatt
     1081 cactattgct gtccagcgga agcctgacag cgttgtaata ttcaattcgg actgactctt
     1141 ctgcgccgca gattccgcag ttctctttct ttttgtcaat gaaccagagt gtgtcttcaa
     1201 tcatataata ttaaacctta ttgtatccta ttcctataac tgtgatgtca tcctgtcttt
     1261 ccctttcccc taaaaaggag tacaggtttt tccttacagc cctgacagtg tcttcaatgc
     1321 tcattgttct gtattccatc agaatagcat acagtctttc ttcgccgaac tcttcctcgg
     1381 aagaattgaa ctgttcaaat gctccgtcag tgaatgcata aattttgtca gatgctgtga
     1441 attccagctc ctttgatgca aattttacat tcttcgtcag accgaccatt gaacctgttt
     1501 tttccagaag atccttggag ttttttctca ctatgactgc agccgggtgt cctgcagaag
     1561 catatgtaat tctctgattc tgcaagtcta tgtcgatgat gatgcaggtc agaagggcgt
     1621 tcaaatagga atatttatcc gtaaattcat tattgaatat ggatagaata tctgcagtcc
     1681 ccagatggaa ttcacgtata ttgtcataaa ttcccttcac tgccattgtg atgagtgcgg
     1741 cctgaacacc gtgacctgtt gcatctgcaa gaaagaatcg gaatctccat tcatcaagtc
     1801 tgttgatgct gtaatagtct ccgcctacct ccgagatcgg gatataaaga ggtacaatat
     1861 ccatttttgt cattatgctg taatgtatat taagaagtcc ctgctgaatc ttgcctgcaa
     1921 gggaaagatc ctgcctgatt gtggcaagtg tacttttcag ttcttctgtc ctctgaagca
     1981 ctttttcttc cagtgtttct gtatgtctga tctgggtttc gaggagtgtt ttctgcactc
     2041 ttacattttc ttcctgtatg aatttgtatc tgtctgcaag cgcgagtgaa agaaagacaa
     2101 gctctatcat ggttcctgcc tgcattgtat agtgagtgac cagattcatt cccaggaatc
     2161 cgagtgaacg cagaagtacc ataaagactc cgcagaaaaa aacaccccag gccatcacaa
     2221 agaatcttgc agacttattg cctcgtttcc atgatttgaa accgcagaaa agcagaagga
     2281 gaacgcttgg agctccgaat atgctctgaa gtatcagtgc cgtcataatc gagtgaaaga
     2341 gtgccagaag tgagagtgat gtgataacct ccatcattct cacagtcctg tgaagggccg
     2401 gtgaaagctc cttaagatgc agaaatcgca gggtgaattc tgcagtgaaa atccatgaga
     2461 aatgcacgaa gaccggaact gcataattct gaaacttagg actgtcgggc cagagatact
     2521 gaaatccgtg accgttgaag cagagatagt aaaccaggaa cagaagcgta cttagagaat
     2581 acaggagata ggtcagactg cggaaagcaa tgcagatgaa tgcattgtat ataatcatta
     2641 cagtaagtac tccataaaat aaaaaatgcc aaccttcggt gagcattccg tctgtacgaa
     2701 gctcatcctt agtgtatagg gtgaggggaa ctgtgatgga actggaagaa tggattttca
     2761 gccatacaga agtttctttt cccggtgcaa agttcatggg tattatgaaa ttcctgtgat
     2821 ttatctcacg ggagtaaaac ggataactgt ctcctgcagt tcttttttta tattcggttc
     2881 cttcctgtgt gtagatttcg atatgatcca gtatgggaaa acttatgctg agaacccagt
     2941 ccctctgctt ttccgattct tcgaatacct tgaacccaat ccagactggg tcctttttga
     3001 atcccagctg aagtattgtg tccacactgg gaataaactg tttctggatt tccggggaaa
     3061 aaatttcagt aaaatttaaa tcttttcctt cctgatagag gaaattcatt ttttttccaa
     3121 tttcagcctt cctggtattt tcagaaagtc tgacagttct ttctgcgaat acag
//
LOCUS       DAOQGJ010000215         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17967_length_3171_cov_3.362323, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000215 DAOQGJ010000000
VERSION     DAOQGJ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17967_length_3171_cov_3.362323"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PK683_04580"
     CDS             complement(<1..243)
                     /locus_tag="PK683_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNH07755.1"
                     /translation="MYELMHWHPVLKSDDLHSAPVKIRILNHDIVMFRDELGKIGALN
                     DICPHRKMKLSEGSVQNGRLVCPYHGWNLGTDGDCIS"
     gene            complement(236..1363)
                     /locus_tag="PK683_04585"
     CDS             complement(236..1363)
                     /locus_tag="PK683_04585"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH07756.1"
                     /translation="MKKETLIIGAGPAGLAVSALLGKRNIPYTILEKQSSVGAAWRNH
                     YERLHLHTVKEHSHLPMKPFPEDYPRYVSRDLLVKYMEDYAEEFNIRPEFNEEVLSVS
                     KTSSGWKAVSSKSVRNVDTLIVCAGYNHSPKTAEWPGMRTFKGRIIHSRDYRNGEEFK
                     GQNVLVVGAGNTGGEIAIDLHEYGAKPTICVRGPLHIVPREIFGVPMQLMSLIMDRLP
                     LGFADMMSRMILQAVTDDLSEYGIFKPNHGSVSQVVSQEKIPLVDIGTVKLIKSGDLK
                     VTGDIDSFTEDGIWYRNGKKESYDAVILCTGYKPALNRIFNENPELFNDRGYPVKKAE
                     DCGNGLYFLGYTNHLTGFLRYIGVEAEKIAEELAAKGSAYV"
     gene            complement(1360..2013)
                     /locus_tag="PK683_04590"
     CDS             complement(1360..2013)
                     /locus_tag="PK683_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HNH07757.1"
                     /translation="MNENGHFPPPWNLDGEGFLLPFWADKKHCLQSGFISESEKDSYR
                     GGLGAVMLVNYRSSDVGPYFELLFIPGDFHHSGKTYKKITKIYVSSQKSVEEGILNWA
                     IPKELAEFSWKKTDKETKIDIQSEKGSVLTTVLKEKFFSFPVTTSLFPFTLLQKAPES
                     LLATKFIGKGSAKFAGLESYSSNPAHFPDIYSVSSFRLPGLAVSPFALTFPVPEVLK"
     gene            2130..2633
                     /locus_tag="PK683_04595"
     CDS             2130..2633
                     /locus_tag="PK683_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07758.1"
                     /translation="MMIMKQSVILLILAISLSASEIKESEVPSKILNSYQKKFGNSKT
                     KWMKHGTSIEAVSSDSDRYELKVTYDIKTERMSELTEKLNRPNAKSDDSYLSRMIADS
                     VRKKILKDMQERSGKKGFGNYISFQHNFTSGKDAYFLRYMQGKNNAAVTYDEKGKIIY
                     LNENLPR"
     gene            2672..>3171
                     /locus_tag="PK683_04600"
     CDS             2672..>3171
                     /locus_tag="PK683_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNH07759.1"
                     /translation="MQSIFIQASLLFVSVFLIQCTASKQSNLNVHLFSGVDTEGKTVR
                     LSDIKDVRRVALNVYSPTCVPCYKEIPTLNYLYKEYSSGNHLKFFMVVDPYLITESAP
                     TENEEAVTAKASAIMKEETVKRKIALPVLMMKRPFQVVSGQNSTALITGTPETLLFKT
                     DPLILY"
BASE COUNT          947 a          722 c          613 g          889 t
ORIGIN      
        1 agaaatacag tccccatccg ttcctaaatt ccatccgtga taaggacaca caagtcttcc
       61 gttctgcacg gaaccctcgc tgagcttcat ttttcggtga ggacatatgt cattcagtgc
      121 gccgattttg cccagttcat cccgaaacat tacaatatca tgatttaata tcctgatttt
      181 aacaggggca gaatgaagat catcagactt cagaacggga tgccagtgca tcagctcata
      241 catatgcaga tccttttgca gcaagttcct cggcaatttt ttctgcctct actccgatat
      301 atctgagaaa tccagtaaga tgatttgtat atcccaggaa ataaagaccg tttccgcagt
      361 cctcagcttt ttttacaggg taccctctat cattaaaaag ttcaggattc tcattaaaaa
      421 ttctgttcaa tgcgggctta taacccgtgc agagtatcac agcgtcataa ctttccttct
      481 tgccgtttct gtaccagatc ccgtcttcag taaaagagtc aatgtcgcct gtaaccttca
      541 gatccccgct tttgatcagt ttcactgtgc ctatgtccac aagaggaatt ttctcctgac
      601 tgactacctg gcttacgctt ccatgattcg gcttgaagat tccatactca gaaagatcat
      661 cagtaactgc ctgaagaatc attctgctca tcatatccgc aaatcccaga gggagtctgt
      721 ccataatcag tgacatcagc tgcataggga caccgaagat ctctctcgga acaatatgaa
      781 gaggtcccct gacacagatt gtcggttttg ctccatactc atgcagatca atcgcaattt
      841 caccgcctgt gtttcctgcc cccaccacaa gcacattctg tcccttgaac tcttcaccgt
      901 tcctgtagtc cctgctgtga ataattcttc ccttgaaagt ccgcattccc ggccattcgg
      961 cagtcttagg actgtgatta tatccagcac atacaatcag agtatctaca tttctgacag
     1021 actttgaaga gaccgctttc cagccggaac ttgtcttcga tacggacagc acttcttcat
     1081 tgaactcagg acggatattg aactcctctg catagtcttc catatacttc acaagaagat
     1141 ctctggaaac atacctcgga taatcctccg ggaaaggctt cattggaaga tgagaatgct
     1201 ccttcactgt atgcagatga agtctttcat aatgatttct ccaggcagcg ccgacagagc
     1261 tctgcttttc cagaattgta taaggaatat tacgttttcc caataacgca ctgactgcaa
     1321 gtcctgcagg tccggctccg ataatcagtg tttccttttt cacttcaaaa cctccggaac
     1381 aggaaatgtg agtgcaaatg gagatacagc cagaccggga agtctgaatg aagacacgct
     1441 gtaaatatcc gggaaatgag cggggtttga actgtaagat tcaaggcctg caaatttcgc
     1501 gctccccttt ccaatgaatt ttgtagcaag cagactttca ggtgctttct gaagaagagt
     1561 gaacggaaat aaggaagttg tcactggaaa cgaaaaaaac ttttccttca gcacggtggt
     1621 cagcactgac cctttctctg actgaatgtc tatcttcgtt tctttgtcag tctttttcca
     1681 gctgaattca gccagttcct tcggaatagc ccagttgaga attccttctt ccactgattt
     1741 ctgactcgaa acataaatct ttgtgatctt cttgtaagtc tttccagaat gatggaaatc
     1801 gcccggtatg aaaagaagct caaaataagg gccgacatca gacgatctgt agtttaccaa
     1861 catgactgca cccagtccgc ctcggtaaga atccttttcg gattccgaaa tgaagccgct
     1921 ttgcagacag tgttttttgt cagcccaaaa tgggagcaga aatccttccc catcgagatt
     1981 ccaaggcgga ggaaaatgtc cgttttcgtt catgaatgaa tgactcccag aaactgttta
     2041 ttttacaact caaaaaaccg ttcagcatac tgacaattgc agtgtaaact caggattaat
     2101 tcttgcaaac aggagcagtc ttcctgaaaa tgatgattat gaaacagtct gtaattttac
     2161 ttattctcgc gatttccctc agtgcatctg aaattaaaga aagtgaagtt ccctcaaaga
     2221 tactgaattc ttatcagaaa aaattcggga attcgaaaac gaaatggatg aagcacggta
     2281 cttccattga agcagtatct tccgattccg acagatacga actgaaagtg acttatgaca
     2341 taaagacaga aaggatgtcc gaacttactg agaaactgaa cagaccgaat gcaaagtcag
     2401 acgattctta tctgagcaga atgatcgcag actctgtcag aaaaaaaatt ttgaaggata
     2461 tgcaggaaag aagcggaaaa aaagggtttg gaaactatat ctcgtttcag cataatttca
     2521 cttcaggaaa ggatgcctat tttctgagat atatgcaggg aaaaaacaat gctgcagtca
     2581 catatgatga aaaaggaaaa attatttatc tgaatgaaaa cctgccgaga taatccttgc
     2641 ttttaaaagg aacgggaaga attcgtacat tatgcaatcc atttttattc aggcgtccct
     2701 gcttttcgtt tccgtatttc ttattcagtg cactgcatca aaacagtcca atctgaatgt
     2761 gcatcttttt tcaggggtgg atacagaagg aaaaacagta agactttctg atataaaaga
     2821 tgttcgaaga gtcgctctga acgtatactc acctacctgc gttccctgct acaaggaaat
     2881 ccctacatta aattatctat acaaagaata ttcttcaggc aatcatctga aatttttcat
     2941 ggttgttgat ccttacctga tcacggaaag tgcaccgact gaaaatgaag aagctgtaac
     3001 tgcaaaagcc tccgcgataa tgaaggaaga gacagtaaaa agaaaaattg cacttcctgt
     3061 tcttatgatg aaaagaccgt ttcaggttgt ttcaggacag aattcaacag cactgatcac
     3121 aggaacacca gaaacacttt tattcaagac agaccctctt atactttact a
//
LOCUS       DAOQGJ010000216         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17971_length_3171_cov_2.613607, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000216 DAOQGJ010000000
VERSION     DAOQGJ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17971_length_3171_cov_2.613607"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..489
                     /locus_tag="PK683_04605"
     CDS             136..489
                     /locus_tag="PK683_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07760.1"
                     /translation="MKRSAMANFLLLSCFSPQFKKAKVIQPDGLALKDRPSMNGKQLA
                     LMKEKEEVLVQRDDGPEETLYGRKSRWFEVEYILRGGYWGSAAQNIRVSSRNFSIPDG
                     RYISVGFRLVFLPED"
     gene            873..1424
                     /locus_tag="PK683_04610"
     CDS             873..1424
                     /locus_tag="PK683_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07761.1"
                     /translation="MKQLIFLCILSLLTAGQISAERIIATGAPSIISEFTKVEELTKN
                     KGITEEFRKKTLEKNLLNAVKGTLLKKFPDYQKKTEDLKDMSSIAFEQQKGTFNYYVK
                     YVTKKTEKDKDGKDSAPYIFYLFYNFAVDPEIYVQLPVDDRIYISTPELEALGNASSS
                     KTPAAPTNSKPADPIMEGKKANP"
     gene            1339..2250
                     /locus_tag="PK683_04615"
     CDS             1339..2250
                     /locus_tag="PK683_04615"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNH07762.1"
                     /translation="MHLQARLLQLRPTPNRLILSWKERKQILNFFQNSLPLFREIGEE
                     IKSIYTSDYKVTWKKKDDPLTEADLFVHTAVKNFLIKKYPDIPLLSEEEDSSIDFSKV
                     PEFFSLDPIDGTREFVNKNPEFAVSLGFVKNGCPILGIVYNPITDEFIYGKTGDGIGR
                     IYLNQTVSKLSFRRNSSERKYRPDHILVSKSEHREGLYSDSFWKDQGNIVPKGSIAYK
                     LALSALSESDICISLKPKNIWDICAGTAIINASGGFSVELKSFCEINYFNPAVTAPGI
                     LAGNPFIVMDYIRMYKKELSERRKEGW"
     gene            2244..3128
                     /locus_tag="PK683_04620"
     CDS             2244..3128
                     /locus_tag="PK683_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HNH07763.1"
                     /translation="MVIRTESSFLELDLQNGGRILCLELISLKGRRRKVIWADPALDF
                     FFSGSFLMFPWVNRLESDRFNINGNRIEIEPVLKDEAGYPIHGLYFKSERRCVSVSEG
                     IESSEIIIEPIQTDSLFPSFRETFSLERSRLTVSLEVFGSSDKEMYFSAGYHPYIGLE
                     GRPIDSLEIISNCTETVLLGDDLLPETPIRKKPFKSAVQTGKIDTLKLDHCFTGNSSH
                     PYFGIFDRTENEKVILEIHRSFNPSVFFQIYTPPHRQSIAVEPMSSLGNSFSEKNGTH
                     SSIKKNDIKVFSFSVRLE"
BASE COUNT          964 a          605 c          690 g          912 t
ORIGIN      
        1 cttatctgtt tgtcaggacg tttcaggtgt cctgaggcgg atgccaattt caagcaggca
       61 ataaagggtt tgtcgctgca tcctggtatg cgatactttg caattacgaa tttgggaaat
      121 ataattggag ctgttttgaa aagatcagca atggcaaatt tcctgctttt aagctgtttt
      181 tccccgcaat tcaagaaagc taaagttatt cagcctgatg gtcttgctct taaagacaga
      241 ccgagtatga atggaaagca gctcgcactg atgaaggaaa aagaagaagt acttgttcag
      301 agagatgacg gaccggagga gactctctat ggcagaaaat ctagatggtt tgaagtggaa
      361 tacattctgc ggggaggtta ttggggcagt gctgcgcaga atattcgtgt ctcaagccga
      421 aatttctcta ttcccgacgg tcgatatatt tctgtcggtt tccgccttgt gtttcttccg
      481 gaggattagc cgacggttca aatttatgaa aaccgccgca aaatcataag gtgcttcacc
      541 accgactgtc tgactttcgg caggtatctg aaccctgttg taaaaaggcc ctccgtgatg
      601 aaatctggct tttacgcaca gtctcgggct gattagcgtt ctctgctgaa atcaggaaag
      661 tttttcttca aatccccctt tccccttggt ggtttatttt ttctaccata tgggggacac
      721 acgggatgat atggatgata agcgtctttt tcagtatgaa cggctttcgc cgcagtcact
      781 gaattttgtc cggggtcaaa aaataagttt gatttcatac gagaattccg ataatcggta
      841 aagtaagaca ttaactgaag gtagcctcat ttatgaaaca actgattttc ttatgtattt
      901 taagcttgct cactgcggga cagatttctg cagaaaggat tattgccaca ggagcaccga
      961 gcattatcag cgaatttact aaggtggagg aactgactaa aaataaagga atcactgagg
     1021 aattccgcaa aaagactctg gaaaaaaatc tgttgaatgc tgtaaaaggc actttattga
     1081 agaaattccc tgactatcag aaaaaaacag aagatctgaa ggatatgagt tcaattgcat
     1141 ttgaacagca gaaaggaaca ttcaactatt acgtaaaata tgtaactaaa aagacagaaa
     1201 aggataagga cggaaaagac tcagctcctt atatttttta tctgttttac aattttgcag
     1261 ttgatcctga aatctatgtt cagcttcctg tagacgacag aatttatatc agcactcctg
     1321 aacttgaagc ccttggaaat gcatcttcaa gcaagactcc tgcagctccg accaactcca
     1381 aaccggctga tcctatcatg gaaggaaaga aagcaaatcc ttaatttttt tcaaaattct
     1441 cttcccctgt tcagagaaat tggggaggag atcaaatcaa tctatacttc cgattataaa
     1501 gttacttgga aaaaaaaaga tgatccgctc acagaggcgg atctttttgt acatactgcc
     1561 gtcaaaaatt ttctcataaa aaaatatcct gatatccctc tgctgtcaga ggaagaggat
     1621 tcgagtattg atttttcgaa agtgcccgag tttttttccc tagatccgat tgacgggaca
     1681 agagaattcg taaataaaaa tcccgaattt gcagtcagtc ttgggtttgt gaaaaacggc
     1741 tgtccaatcc tgggaattgt ttacaatccc attactgatg agtttattta cggaaaaaca
     1801 ggggatggga taggcagaat ttatctgaac cagactgtct caaagctgtc ctttcgcagg
     1861 aacagttcag agagaaaata cagaccggat catatccttg tatccaagtc ggagcacagg
     1921 gaagggctgt attctgattc tttttggaag gatcagggga acattgttcc aaaaggttcc
     1981 attgcatata aactagccct gtcagccttg tctgaatctg atatctgcat atcccttaaa
     2041 ccgaagaata tctgggatat ctgtgcaggc acagccatca tcaatgcatc cggaggattc
     2101 tccgttgaac tgaaaagttt ttgtgaaata aattatttca atcctgcagt cactgccccc
     2161 ggaattctgg caggaaatcc attcatcgtg atggactata tacgaatgta taaaaaagag
     2221 ctttcggaaa gaaggaaaga gggatggtaa tccggacgga aagttcgttt ttggaactgg
     2281 atcttcagaa cggggggaga attctctgtc tcgaactgat atcactgaaa ggcagaagaa
     2341 gaaaagtaat ctgggcagat cctgcattgg atttcttttt ttcagggtct tttctgatgt
     2401 ttccctgggt aaaccgactg gaatccgacc gattcaatat caatggaaac aggatagaaa
     2461 ttgaacctgt tttaaaagat gaagcagggt atcctattca tggactgtat tttaagtcag
     2521 aaagaaggtg cgtttctgtt tcagagggca ttgagagttc tgaaatcatc attgaaccga
     2581 ttcagacaga cagcttattt ccatcctttc gggaaacgtt ttctctggaa aggagcagac
     2641 tgacagtcag tctggaagta ttcggttcca gtgataaaga gatgtatttt tctgccggat
     2701 atcatccata cattggcctt gaaggcagac cgatagacag tctggaaatt atttccaact
     2761 gtacagaaac agtgcttcta ggggacgatc ttcttccgga aactccgatc agaaaaaagc
     2821 cttttaaaag tgcagttcag acaggaaaaa tagacactct gaagctggat cattgcttta
     2881 ccggaaattc gtcccatcct tattttggaa tttttgacag gacagagaat gaaaaagtaa
     2941 tccttgagat tcacagatca ttcaatccaa gtgttttttt tcagatttac actccgcctc
     3001 acagacagtc gattgcagtg gaacctatga gcagtctggg aaattccttt tctgaaaaaa
     3061 atggaactca ttcttcaata aaaaagaacg acatcaaagt tttctctttc tctgtccgat
     3121 tagaatagat atgtatctgg aaccgttcaa gaatattgat tttatgcttt c
//
LOCUS       DAOQGJ010000217         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_17991_length_3167_cov_3.586118, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000217 DAOQGJ010000000
VERSION     DAOQGJ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17991_length_3167_cov_3.586118"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK683_04625"
     CDS             complement(<1..232)
                     /locus_tag="PK683_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNH07764.1"
                     /translation="MKRKKIAVIGLGDFGKVLVKCLYEEKHEVVAIDMEMDLIEEIKD
                     HCTDAICMDATDENAMRSQGLEDFDYVILAVAD"
     gene            complement(232..2124)
                     /locus_tag="PK683_04630"
     CDS             complement(232..2124)
                     /locus_tag="PK683_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH07765.1"
                     /translation="MTKFHSTDIRNGLMVPYDDIYIFPISEPVFAFLEKVWSSFVNKM
                     VGLSFFNQENMFDHAVFEVIQGSEMKIVASATHFRMKELIGKRYNKDIADFISKTKPI
                     PLNDEKNESARILQKAVLAYSHMQRPEVEFMDLRNNEKVHPNLQILLTDTMRYSVGIP
                     LIVKEMPVGILWGIRRKELTEEQKRDIILQLHTLFDVIEYVVALEVDRKGDDYFARKN
                     IEKADTTSSIHHLLYTVQPTQKDPVTSIIAHSHVYNMMYRLDASYMVPTSHGFTISLK
                     HFAPEQMNDSKVIALMIPGFFCRRSVMDKVAREMALRYGYRVYSMDLRGRSRYTLPKD
                     GTKYSWSLDDYIHDDFPAVIRWIKDNHPDHKILVVGHSMGGMIPRFYAGSYDKIRKMP
                     GKGDKINPDENIVGMVSITSPSYMDLGSNIMGLGILKNSSKLINNRTVYDILFNLVSF
                     PIRNTISSIDLNGFFKFVLNIHSSLRAFSYQVSTQVVNINDFVGYKQISPPEWYFMVE
                     DVFCEESIKVILQFVRSQLSAEQAFYSYDGSMNYTADLSKIKIPVLSIAGTVDKIVPS
                     HTIESIMDIQNTPYKKLLRYEQGHLGIIFHQETVNSICEEIHNWVEEITGKSHPVPEK
                     LAAAFI"
     gene            complement(2197..>3167)
                     /locus_tag="PK683_04635"
     CDS             complement(2197..>3167)
                     /locus_tag="PK683_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07766.1"
                     /translation="VYTFPDGDEYSGDFKDDKFSGQGSYKYASGDLYTGEFRNDKFHG
                     KGRLVMSNGDIYEGGFANGAKEGNGSYQFANKSRYTGEFLKDRFNGKGVYVFANGDVF
                     EGEFRDDKYFGQGQYTYANGTKYAGMFNNDKFNGGGSIVNADGTRYVGEFKDDLYHGK
                     GTLTYQNGDSYSGDFRAGLYHGRGTMMYSSGDKYQGEFAYGKRHGTGVYTYANKDKYI
                     GEFKDDVFSGKGLYHFSDGSIYEGQFENDRYNGKGIYTYSSGDRYEGEFLDDMRHGYG
                     TYVTVDGIKYVGEYKNDLKNGQGILSDKSGRVIYSGQWVEGQQANK"
BASE COUNT          886 a          740 c          569 g          972 t
ORIGIN      
        1 tgtctgcaac ggcaagtatg acataatcaa aatcttcaag accctgagaa cgcattgcat
       61 tttcatctgt agcatccatg cagatggcat ctgtacaatg atccttgatt tcttcaataa
      121 ggtccatttc catatcaatg gcaacgacct catgtttttc ctcgtacaga cattttacga
      181 gaaccttgcc gaaatctcca agaccgataa cggcaatttt ttttcttttc atcagatgaa
      241 cgcggctgcc agtttttccg gaactggatg gctcttgcct gtaatctctt ccacccagtt
      301 atgaatctcc tcacagatgc tgtttacagt ttcctgatgg aatatgattc caagatgtcc
      361 ctgctcgtaa cggaggagtt tcttataagg agtgttctga atatccataa tagattctat
      421 tgtatgagat ggtacaatct tgtccacagt gcccgcaata gagagtaccg gaattttgat
      481 tttggaaaga tccgctgtat agttcattga accgtcatag gaataaaatg cctgctcggc
      541 agacagctgc gatcttacaa actgcagaat gaccttaatc gattcttcac agaatacatc
      601 ctccaccata aaataccatt caggaggaga tatctgcttg taacccacga agtcgttaat
      661 attcacaacc tgtgtactga cctgatagga aaatgccctg agtgaagaat gaatattcag
      721 aacgaatttg aaaaatccgt tcaagtcgat ggaagatatt gtatttctga tcggaaaaga
      781 aaccagattg aaaagtatat cgtatacagt tctgttgttg atcagcttag aactgttctt
      841 aagaattccc agtcccatta tgttggaacc gagatccata taactcggtg aggtgatgga
      901 aaccattccg actatatttt catcaggatt gatcttgtct ccctttccgg gcatcttcct
      961 gattttatca taacttcctg cataaaaacg aggaatcata ccccccatac tgtgaccgac
     1021 aacaagaatc ttatgatccg gatgattgtc cttgatccat ctgattactg ctggaaaatc
     1081 atcgtgaata taatcatcca gagaccagct gtattttgtc ccgtctttag gaagagtata
     1141 tctggaacgt cctctcagat ccatactgta gacacggtac ccgtatctga gtgccatttc
     1201 tctggcaacc ttgtccatga ccgaacgtct gcagaagaat ccaggaatca taagtgcaat
     1261 tacttttgag tcattcatct gttcaggggc aaaatgcttg agactgattg taaagccgtg
     1321 agatgtagga accatataac tcgcatcaag cctgtacatc atattgtaga catgagagtg
     1381 agcaataatg gaggtgaccg gatctttctg agtaggctga acagtgtaaa gaagatgatg
     1441 aatggaagaa gtagtgtcag ccttttctat gttctttctt gcaaaatagt catccccctt
     1501 ccggtcaact tcgagggcga ccacatattc gatcacgtca aacagtgtat gcagctgaag
     1561 aataatatct cttttctgct cctcagtaag ttcctttctt ctgatacccc agagaatacc
     1621 tacaggcatt tcctttacta tcagaggaat tcctacagaa tatctcattg tatcagtcag
     1681 aagaatctga aggttggggt ggactttttc attatttcta agatccatga attccacttc
     1741 aggtctctgc atatgactgt atgccaaaac tgccttttga agaattctgg cagattcatt
     1801 tttttcatca tttaatggga tcggttttgt cttgctgata aaatccgcaa tatctttatt
     1861 gtatcttttt cctatcagtt ccttcatcct gaaatgagtc gcagaagcaa cgattttcat
     1921 ttcacttccc tggatgactt cgaagacagc atgatcgaac atgttctcct gattaaaaaa
     1981 ggaaagaccc accatcttgt ttacaaagga cgaccagacc ttttccaaaa aagcaaaaac
     2041 aggttcactg atcgggaaga tgtagatatc atcataagga accatcagac cgtttcttat
     2101 atctgtacta tgaaactttg tcataaaaat ttctcacttt actgcatatt tttcagatac
     2161 atttctgcac ctgcgaatct tacaaatcat gaaatgtcat ttattcgcct gctgaccttc
     2221 tacccattga ccggaataaa ttacccttcc acttttatca gagagaattc cctgaccgtt
     2281 cttgaggtca ttcttatact caccgacata tttgatccca tctactgtta cataagttcc
     2341 atacccgtgt ctcatgtcat ctaaaaactc gccttcataa cggtcaccgc tggaataagt
     2401 atagattccc tttccgttat agcggtcatt ttcgaactgt ccttcataga tgcttccgtc
     2461 agagaaatgg tacagccctt tgccgctgaa tacatcgtct ttgaattctc cgatgtattt
     2521 atccttgttc gcataagtat aaactccggt tccatgcctt tttccgtatg caaattcacc
     2581 ctgatattta tcaccggagg aatacatcat tgtgcctcga ccatgataca gacctgcgcg
     2641 aaaatccccg gaataagaat ccccgttctg ataggtgaga gtccctttgc catgatataa
     2701 atcatcctta aactcaccga catatctggt gccgtctgca ttgacaatcg acccgccgcc
     2761 gttgaattta tcattattga acattccggc gtattttgta ccgtttgcat aagtgtactg
     2821 gccttgaccg aaatatttgt catctcggaa ttcaccctca aagacatctc cgtttgcaaa
     2881 cacatagaca ccctttccat taaatctgtc cttcagaaat tccccagtgt atctgctttt
     2941 attcgcaaac tgatacgacc cattcccttc ctttgcaccg ttcgcaaacc ctccctcgta
     3001 aatatcaccg tttgacatca ccaggcgtcc cttgccgtga aatttgtcat ttctgaattc
     3061 acctgtgtag agatcaccgg atgcatattt ataagagcct tggcccgaaa acttatcgtc
     3121 cttgaaatcc cctgagtatt catctccgtc aggaaaagta tagaccc
//
LOCUS       DAOQGJ010000218         3166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18007_length_3166_cov_2.495661, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000218 DAOQGJ010000000
VERSION     DAOQGJ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18007_length_3166_cov_2.495661"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PK683_04640"
     CDS             complement(<1..1635)
                     /locus_tag="PK683_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07767.1"
                     /translation="MMIGFCIFLSHCSLFEKNIKVKPGDFDYFSISKNYFQPPVNRPF
                     PLTVQKGNNLYSSVTADGRYMYFTSDKKGNQDIYLRDLNSSVVVQITSHPASEFKPAV
                     SPDGKKLAFVSTKFDPDGDLVLMDVNPEKTVKDSLKGIYPNEEFENFLTAKGGEDRFT
                     DTDPVFTPDGKSILFVSDRFTPGLPNLVLLTLGKKEMIPLTKDGAASPRFSDDGRYIL
                     FISRKDDIHGEVYRYDRVSGVTERLTSDSYPDFSPSISPDGNYAFFVSIRKDSNGNGI
                     LDERDESVIVRKDLRSKKEKILSAPAVSAFDAKYSGFNGGSIVYSAPKFNAINIYFIP
                     VTGSIAKQENIVEQFRYAERYRDDPEPENYRLALDSIDFYFSEDPLFPLYKSQYEYET
                     VLKEISRKTRLEKTARLEEELYRKGDRLFGILIQCHLKKLQGKDCTKVLETASSEKNL
                     ADWKDKDTAPFVLSLAGQEAERVSNFQEAEKKYSEIIKKYPDYHQINSVREKIGELSM
                     LKKGSGWLPEMFREILASGDSGFEDRKTVLNALEKSI"
     gene            complement(1700..2806)
                     /locus_tag="PK683_04645"
     CDS             complement(1700..2806)
                     /locus_tag="PK683_04645"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH07768.1"
                     /translation="MNPKDLLSIQPNSETDLSEKDRTIRLLEKQIQETEKKAGILRKM
                     VDSSSFTCSVFDSQYRIVEINHLAAEVTKAITKRDVRTGDSILDYVSPIETPDFLKNS
                     KIALEGKTVEVERSIKGPDSQTLWYQFTYTPFLSESGETLVIFTGNDITHKKRSLEIL
                     QTANEGLEALIKRKNEELQSYHRLFDRKINEEQRKIFQSSSETGLLEKQRILSVLDPG
                     SEAFRFGISSSVLSNIFRTDTDSFTLNSSFLEIICRMASSVSELRISVSSEEIEIRNS
                     PGFFSEAVWQTVSAASLFFPSELNIQCIRNSNTAELRFGFRKSVQEFKDHSAESCWTF
                     QLGKARFILENFFCGKISILQETKETVYLLTIPV"
     gene            2915..>3166
                     /locus_tag="PK683_04650"
     CDS             2915..>3166
                     /locus_tag="PK683_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07769.1"
                     /translation="MFSDSAEKSGSGISIHLIVLAVLVFFDRSYSKEKKNPDHFKPQF
                     IQAEAEEDFESLENAVSDLPVFSESNGRTLKTSHLVPEVD"
BASE COUNT          838 a          674 c          667 g          987 t
ORIGIN      
        1 gatggatttt tccagagcgt tcagaacagt ttttctgtct tcgaatcctg agtccccgga
       61 cgcaagaatt tccctgaaca tttcaggaag ccatcctgat cctttcttca gcatactgag
      121 ctctccgatt ttttcacgga cagaattgat ctgatgataa tcgggatatt ttttaatgat
      181 ttcagaatat tttttttccg cttcctgaaa attggaaacc ctttccgcct cctgaccggc
      241 cagggaaaga acaaacgggg cagtgtcctt atccttccag tcagccagat ttttttccga
      301 agaagcagtt tcaagaacct ttgtgcagtc ttttccctga agttttttca ggtggcactg
      361 aatcagaatt ccgaaaagcc tgtctccttt cctgtacagt tcttcttcaa gtcttgcggt
      421 tttctcaagt cttgtctttc ggctgatttc cttcagtact gtttcatatt catactgaga
      481 cttgtacagt gggaacaggg gatcttccga aaaataaaaa tctatggaat ccagtgcaag
      541 gcggtagttt tccggttcag gatcgtctct gtaacgttca gcataacgga actgttccac
      601 aatgttctcc tgctttgcaa ttgaaccggt aactgggata aaatatatat tgattgcatt
      661 aaattttggt gcagaatata cgatgcttcc cccgttgaat cccgaatact ttgcatcgaa
      721 ggcagaaact gcaggtgcac tcagaatttt ctcctttttg ctccgaagat ccttccttac
      781 aataaccgat tcatcccttt catccagaat tccgttaccg ttgctgtcct tgcgtatgct
      841 tacgaagaat gcatagtttc cgtcaggact gatgctcgga gaaaaatcag gataggaatc
      901 ggacgtcagt ctttccgtga cgccggacac tctgtcatac ctgtagactt caccgtgtat
      961 gtcgtccttt cttgaaataa aaagaatgta tcttccgtcg tctgaaaaac gcggagaagc
     1021 agctccatcc tttgtcaggg gaatcatttc ctttttccca agcgtgagaa gcaccagatt
     1081 cggaaggccc ggagtaaatc tgtcggacac aaaaagaatg gacttcccat cgggtgtgaa
     1141 gacaggatcc gtgtcagtga atctgtcttc tcctcctttt gctgtcagaa aattttcaaa
     1201 ctcttcattc ggatagattc ctttcagaga atctttgaca gttttttccg gattgacatc
     1261 catcagaacg agatcgccgt cgggatcgaa tttcgtggat acaaatgcaa gtttttttcc
     1321 gtcgggactg acagccggct tgaattccga agcaggatga gaagtgatct gcacgactac
     1381 ggaagaattg agatcacgca gatagatgtc ctgattgcct tttttgtccg aggtgaagta
     1441 catatatctt ccgtccgcag tgacggaact gtaaaggttg tttcctttct ggaccgttag
     1501 aggaaagggt ctgtttaccg gaggctggaa atagttcttt gaaatggaga agtagtcaaa
     1561 gtctccgggt ttcaccttga tgttcttttc aaacagagag cagtgggaaa gaaaaatgca
     1621 gaacccgatc atcataagtc tgatgcctgc actttctgtg aattgggtaa ttttttttaa
     1681 gagcaaatca aatccttgtc tagacaggaa ttgtaagaag atatacagtt tccttggttt
     1741 cctgtaaaat ggaaattttt ccgcagaaaa aattttccaa aatgaatctt gccttgccta
     1801 attggaaagt ccagcagctt tctgcggaat gatctttgaa ttcctgtact gattttctga
     1861 acccgaaacg cagttcagca gtattggaat ttcggatgca ttgaatattc aattcagacg
     1921 gaaaaaataa cgacgcggca gaaactgtct gccatactgc ctcggaaaaa aaacctggag
     1981 aattacggat ttctatttct tcagagctga cgctgatcct taattctgag accgaggacg
     2041 ccattctgca gatgatttcc agaaaagacg aattcagcgt gaatgaatcc gtatccgttc
     2101 tgaatatatt ggaaaggaca gaagaactga tgccgaaacg aaaagcctca ctacccggat
     2161 ccaggacaga aagaatccgc tgtttctcca gaagtccggt ttccgaagaa gactggaaaa
     2221 tctttctctg ttcttcattg atttttctgt caaaaagtct gtggtaggac tgaagttctt
     2281 cattttttct tttgatcagt gcttccagtc cttcatttgc agtctgcaga atttccaggc
     2341 ttcttttttt gtgagtgata tcattgcccg taaatatgac cagggtttct cctgattcgg
     2401 aaagaaaagg agtatatgta aattggtacc agagagtctg agaatccggc ccctttatgc
     2461 ttctttccac ctccacagtc tttccttcca gtgcaatttt cgaatttttc aaaaaatcgg
     2521 gggtttcgat aggactgaca tagtccagaa tactgtctcc tgtgcggaca tctcgttttg
     2581 tgattgcttt tgtcacttca gcagcaagat gattgatttc tactattctg tactgactgt
     2641 cgaatacact gcaggtaaaa ctgcttgaat ccaccatttt ccgcagaatt ccggcttttt
     2701 tttcggtttc ctgaatctgt ttttccaaaa gtctgattgt acggtctttt tcagaaagat
     2761 cagtttctga atttggctga attgacagga gatctttagg attcataaaa agaatagagt
     2821 caatttttcc ccttttccgc ttctgtaaag cgaatttttt gattgatcag aaattcttgc
     2881 tgcataagat cggctccttt ccgaaaatag tactgtgttt tcagactcgg cagaaaaatc
     2941 aggttcaggc atcagcattc atctgatcgt actggcagtg cttgtattct ttgacagaag
     3001 ttattccaaa gaaaaaaaga atcctgatca ttttaagcct caatttattc aggcagaagc
     3061 ggaggaggac tttgagtctc tagaaaatgc cgtttcagat ctgcctgttt tctctgaatc
     3121 aaacggcagg acgctgaaga cttctcatct tgtccctgaa gtggac
//
LOCUS       DAOQGJ010000219         3161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18062_length_3161_cov_3.254346, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000219 DAOQGJ010000000
VERSION     DAOQGJ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18062_length_3161_cov_3.254346"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="PK683_04655"
     CDS             complement(<1..1540)
                     /locus_tag="PK683_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNH07770.1"
                     /translation="MKRSIRLYILDYMQSFIFFSAVFLTAFWFQAQALYSEEFLVIFP
                     YKNFIYEQNLTGFQSQFIGRDQTREIFLNETDQETMQKIISSEGRSEKLTVIAFGPES
                     LNFARQNFKSSRTVFSMVNSARSFDLETGNICGYENVFPYKNYFKVIKEIKPDSKKIK
                     TVYSSAQGEYESRSGEYQDLEYALILQHKKVSPEDDFRREVLEFIKGADAFIITNDPI
                     FNDKNFRMISEFCRENGILLLTNHIPLVEAGASIGIVPDYTRLGELTAELANGLISGK
                     TECRFGPFLSYSGDILYINETYLKKSGFELSEGFRTKAEIFRQISSGIELFNKQLYKS
                     AQKTFESILKKGPNETASYYLKRTVEKRTYDETSRLVIEAERLLKDRKYVQSRMICRK
                     ILSLNSSHIQARELFSRVTAEESEEIRKEAENSAKSGKPFIAIKRYKESLNIFPENRK
                     ARDGMEAVRRNELKRFPEYFRTGLEFYQKRKYKEAVDTFENTLLLDPAHQNSIEYLRL
                     SKLKY"
     gene            complement(1688..2275)
                     /locus_tag="PK683_04660"
     CDS             complement(1688..2275)
                     /locus_tag="PK683_04660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH07771.1"
                     /translation="MWDERYSADEYAYGKAPNRFLEENFSSIPKGKVLSLAEGEGRNA
                     VFLAKQGYSVTAVDGSSVGLKKAEKLAAENNVRIECIQADLAEFDLGQNKWEGIVSIY
                     CPLPSVLRKEIYKKVILALKQNGVFFLEAYTPDQIGKGTGGGSDADTMQSKQSLSAEL
                     AGLEFKHLIELEREVVEGIYHTGLGSVVQMIAMKK"
     gene            complement(2420..>3161)
                     /locus_tag="PK683_04665"
     CDS             complement(2420..>3161)
                     /locus_tag="PK683_04665"
                     /inference="COORDINATES: protein motif:HMM:NF027222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNH07772.1"
                     /translation="FVEGFLGGGLTDWLMKDDENEMYYIILLNSDILKKSFSEWENEK
                     YSSAFMKSAGDDGYRLKIQTDDSIKAFCHILIHETAHIVDSENTETPYSHIFHAQYRR
                     GLGKTVPSSTEFTSPVWISFSSPIEEYEYAFRDEINFYAMNQKRPILNNNLMLPVFES
                     LKKTGFVSLYGAKTWTEDFAEISAFYFMKNILKIDYKVNIYNGNSIVYEYEPMKSEKA
                     ISRFRHIEKYYSGKNSGIFRRMKKDSWK"
BASE COUNT          873 a          710 c          529 g         1049 t
ORIGIN      
        1 catatttcag cttggacagt ctgagatatt caatggaatt ctgatgagca ggatcaagca
       61 gaagtgtatt ttcaaaagta tccactgctt ctttgtattt tcttttctgg taaaactcca
      121 gccccgtcct gaaatactcc gggaaccttt tcagctcatt tcttcggaca gcttccattc
      181 catccctggc tttacggttt tccggaaata tatttaatga ttctttatac ctttttattg
      241 caataaacgg tttccctgac ttggcactgt tttccgcttc tttccggatt tcttcgcttt
      301 cttctgcagt gactcttgaa aaaagttccc ttgcctggat atgagaactg ttcagagaca
      361 gtattttacg gcagatcatt ctggactgca catatttcct gtctttcaaa agccgttcag
      421 cctcaattac gagtctggag gtttcatcat aagtcctttt ttcaacagtt cttttcagat
      481 agtacgaagc tgtttcattg ggacctttct tcagaatgct ttcaaatgtt ttctgtgcag
      541 acttgtaaag ctgcttgttg aaaagttcaa ttcctgaact gatctgtctg aaaatttccg
      601 ccttggttcg gaaaccttca ctcagctcga aaccggattt tttcagatag gtttcattaa
      661 tatagagtat gtctcctgaa taggaaagaa acgggccgaa cctgcactct gtttttcctg
      721 atattaatcc atttgccagt tcagctgtca gttcaccaag tcttgtatag tcaggaacaa
      781 ttcctatgga agctcccgct tcgacaagag ggatatgatt tgtcaggagg agaatcccgt
      841 tctcccggca gaattcagaa atcattctga aatttttatc attaaagatt ggatcatttg
      901 taattataaa tgcatctgca ccctttatga attcgagaac ttctctgcga aagtcatctt
      961 ccggagaaac ttttttatgc tgcagaatca gtgcatattc aaggtcctga tattcaccgc
     1021 tccttgactc gtattctccc tgggcagagc tgtatacagt cttgattttt tttgaatcag
     1081 gcttgatttc cttgatgacc ttgaaatagt ttttataagg gaaaacgttt tcataaccgc
     1141 agatgtttcc agtttcaaga tcaaaactcc tggctgaatt caccattgaa aaaacagtcc
     1201 tggaactttt aaagttctgc ctggcaaaat tcagagactc agggccgaaa gcaataactg
     1261 tcagtttttc agacctgcct tcggaggaga taattttctg cattgtttcc tgatccgttt
     1321 cattcagaaa tatttcccta gtctgatctc ttccgatgaa ctgagactga aatcctgtaa
     1381 ggttctgctc atagataaag tttttatatg ggaaaattac gagaaactct tctgaatata
     1441 gagcctgtgc ctgaaaccaa aaggctgtca gaaagaccgc agaaaagaat atgaaagact
     1501 gcatataatc caatatatac agtcttatac ttcttttcat caagtactaa tttttaattt
     1561 tttaccctgt ccagccaata cttctggaac tgcggttttc atcagaacaa aacgcatact
     1621 gtctgcagga aaaactgagc cgcaccggca ggaatatgat ctgcagacaa atctgtctgc
     1681 ctgtagatca tttcttcatt gcgatcatct gcacaacgga gccgagtccg gtatgataaa
     1741 ttccttccac tacttcccgt tccagttcaa tcagatgctt aaattcaaga cctgcaagtt
     1801 cagcactcaa tgactgtttt gactgcattg tgtctgcatc acttcctccg ccagtacctt
     1861 ttcctatttg atcaggagta tacgcttcca gaaaaaacac tccattctgc tttaatgcaa
     1921 gaatcacttt tttataaatt tcttttctaa gtactgaagg cagggggcag tatatcgaaa
     1981 caatcccttc ccatttattc tgtcccagat caaactctgc aagatccgcc tgaatgcatt
     2041 caattctgac attattttca gcagccagtt tttcggcctt cttcaagcct acggaagacc
     2101 cgtcaacagc cgtaacactg tatccctgct tcgccaaaaa gactgcattt cgtccctctc
     2161 cttcagcaag gctgagtacc tttccctttg gaatactgct gaagttctct tccaggaacc
     2221 tgttcggtgc ctttccataa gcatattcat ctgcactgta tctttcatcc cacatatttt
     2281 tctccctttc tgatatcgtt ctgcaatcca ttccctaaaa aggatacaat taaatacaat
     2341 atcagttaaa tttttcactc cagtcaaatt gaaactttgc aggcattcaa tatacggatt
     2401 tctgtcagcc tcaatcaatc tatttccagg aatccttctt catccttctg aatatgcctg
     2461 aattcttccc ggaatagtat ttttcaatat gtctgaacct gcttattgct ttttcggatt
     2521 tcatcggctc atattcataa acaatagaat ttccattata tatatttact ttataatcta
     2581 tttttaatat atttttcata aaataaaaag cagatatctc tgcaaagtct tccgtccacg
     2641 ttttggcacc gtacagagaa acaaatcctg tctttttcag agactcaaat actggaagca
     2701 tcagattatt attaagaata ggacgtttct gattcatcgc atagaaattg atctcatcac
     2761 ggaatgcata ttcatattct tctattgggg atgaaaagga aatccatacc ggtgaagtaa
     2821 attcagtact gctcggcact gtttttccca accccctcct gtactgtgca tgaaaaatat
     2881 gactgtaagg tgtctcagta ttttcactgt caacgatatg agccgtttca tgaataagta
     2941 tatgacagaa agcttttatg gaatcatctg tctgaatttt cagtctgtac ccgtcgtcac
     3001 cggcagattt cataaaggca gaactgtatt tttcgttttc ccactctgaa aaactctttt
     3061 ttaaaatatc agaattgagg aggattatat agtacatctc attctcatca tccttcataa
     3121 gccaatcagt cagtccgcct cctaaaaaac cttccacaaa a
//
LOCUS       DAOQGJ010000220         3161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18063_length_3161_cov_3.151642, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000220 DAOQGJ010000000
VERSION     DAOQGJ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18063_length_3161_cov_3.151642"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK683_04670"
     CDS             complement(<1..214)
                     /locus_tag="PK683_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH07773.1"
                     /translation="MISLKKKVELEEISAASMSDMAFLLLVFFMVTAVFFVKEGFNIQ
                     LPRKNSKPTEVLRENVFEFQVSGENIV"
     gene            complement(217..849)
                     /locus_tag="PK683_04675"
                     /pseudo
     CDS             complement(217..849)
                     /locus_tag="PK683_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
     gene            1769..>3161
                     /locus_tag="PK683_04680"
     CDS             1769..>3161
                     /locus_tag="PK683_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07774.1"
                     /translation="MKLKLYSIMAAAAFLLYCPSKKDDSSKKNALLGAAFVLSQQYEA
                     TVTVVGFLQETGGAPIRDGKVKIADSTDSSYLTSGQGTIDEFTKCETDNVSALTDVTT
                     TKLDGQFTVTFKVKSASGKVVFSAVRSDTGSSTTACSAIANFTSTQYTKNVGTANFAI
                     TVKNSKETNQLTVSDASGLKLTIKSVETVVKGSYNLSSPTTGENICDGKNVTGTPETI
                     SGDISSNKTISGSAILSGTVFVKSGATLTVDAGSVIFGNRGSSLYILQGGKLVTKGTK
                     TAPVCFTSSQAPGSRYPGDWGGIVMIGNAIGTRSSTTEGTTPQNYGSGTDDTDSSGSL
                     EYTIVEFAGNEVAPGDELNSFSLYTIGSGTKMQYLQAHRGLDDSFELWGGKVTGKYWL
                     ATGGLDDDFDFDEGFQGNLQYLIGHKYPTSCGGSPSTDPHGFEMDGINSGSGQSVCDS
                     PKRCSNPTISDYTV"
BASE COUNT          899 a          655 c          693 g          914 t
ORIGIN      
        1 ttacaatgtt ttccccgctt acctgaaact cgaaaacgtt ttctctcagt acctctgtcg
       61 gcttggagtt tttcctaggg agctgtatgt tgaatccttc cttcacgaag aatactgcag
      121 tcaccataaa aaagaccaga agaaggaagg ccatatccga catggatgca gccgatattt
      181 cctccagttc tactttcttt tttaatgata tcataattat gcctttttag cctgcagctt
      241 cagcagttcc ttgtagatcc tgttcgcagc ctcttcaacg tctgcagcga agccgtttac
      301 cttgcctgtg agaacctgat gaaatgacat gactggaatc gccacgatca gaccggctgc
      361 tgttgtgatc agtgcttcct tgatacctgc cgcgactact ttcgcattta cctgatctgc
      421 atttgcaatt gcatcaaagg cattgatcat accggagact gtacccagaa atccgatgag
      481 cggagctatg gtagatactg ctgccagaat tgcaagtcct ctttccagga gagtgatcac
      541 ttccccagcc tctctttcaa tccccttcac gaagagttct gaatccttct gggaaacatc
      601 caggccgttg atcagtacat ctgaaatttt ctgatctttc gtttcttcca gatatttttc
      661 cgcctcggga agccctccct gttctatttt atctatcagt gcctgattga aaccttttac
      721 tatcagtttt gttctgatga aaaaataggt tctttctatt gctacgccca ttccaatcac
      781 ggatgccagg agcagaggcc acatggacca tccccctgca atgaagagat caatgagtcc
      841 tggggacttc ggctgtgcca tcggttttgc cttttcagtc agatttgccg tttctgccgg
      901 cttgttttcc gttgtttttg caggaactgt tgctgtgttc tgtgcgggaa cagttccatt
      961 ctgagccagt gcaggtgaga tataatataa tgctccacaa agtacgagcg tgtaaataat
     1021 gagccttgta cggattttcg aaaaaatttt cataaattct tgcttcagaa ttcccgaaac
     1081 tctgtttcag aatgcctgag gtccttaatt tcatattcat catatctgat gtctggactc
     1141 agggtagttt ttttctgtta cgaaattatt acgcggaaat tactttttga ggaatgagag
     1201 gtgttttttc actgaacggc gagtaattta ttcccgggaa ctgcggttga gaaaaaaata
     1261 ggctcacgcg ggatgcggag cactgttttt gcgtcgctgt tgatccgatt cattctgctt
     1321 atttttgaaa tcagtacaag aattgaaaga aaaagcagaa aatgcctgca gcttttcaaa
     1381 ttgctgcaat cctttgtatc cggatttttc tgtctatccc gatgtatgcg aaagcattac
     1441 agtcatcggg ataaaagaat ctgctttctt aactgccctt gtcgatcggt gcatggatca
     1501 gaaatgactc aataaaatca atgctcttgg gattttatcc tgtttctgat ctgttcaggt
     1561 tgagaaaaga atataattat aaataaacat ataaaaataa taattccgta aatatattat
     1621 tcgactgtta atcgggagca tccttcgatg taagaaaatt cttccgttca tgaatttata
     1681 tttttccgtc atcagtctgt aacagaaatc cgggataaac agaacagagc tcgaagatat
     1741 tcagagcgaa atctcgcagg agaacagaat gaagttaaaa ttatattcta tcatggcagc
     1801 ggccgctttt ttgctgtact gcccttcaaa gaaggatgat tcatccaaaa aaaatgcgct
     1861 tctcggtgca gcatttgtat taagccagca gtatgaagca acggttacgg tagtagggtt
     1921 cctgcaggaa acaggcggag ctccgatcag agacggaaaa gttaaaattg cagacagtac
     1981 tgattcaagc taccttacat ccggacaggg aacaattgac gagtttacaa aatgtgaaac
     2041 ggacaacgtg tctgctctga cagatgtaac aaccaccaag cttgatgggc agtttactgt
     2101 aactttcaag gtcaagtcag catcagggaa agttgtattt tctgctgtaa gatcagatac
     2161 aggatcaagc acaactgcat gttctgcaat tgcaaacttt acaagcactc agtacacaaa
     2221 gaatgtcggt acggctaatt ttgcaattac cgtaaaaaac agtaaagaaa caaaccagct
     2281 gacagtttct gatgcaagcg gtcttaagct tacaattaag agcgtagaga cagtggtgaa
     2341 gggttcatat aatctatcga gtcctacaac aggagaaaac atctgtgacg gtaaaaatgt
     2401 taccggaact cctgaaacaa tcagcggaga catatcttca aacaagacaa tcagcggaag
     2461 tgcaattctt tccgggactg ttttcgtaaa aagcggagcg actctgacag ttgatgctgg
     2521 atcagtcatt tttggaaaca gggggtcttc tctttatatt cttcagggcg ggaaacttgt
     2581 aacgaaaggt acaaagacag cacctgtatg tttcacatct tcacaggctc caggttcgag
     2641 atatccgggc gactggggag gaattgtgat gattggaaat gcgataggta caaggtcatc
     2701 cacaactgaa ggaacaactc ctcaaaacta tggaagcgga acagatgata ccgacagttc
     2761 aggatcttta gaatatacaa ttgtagaatt tgcaggaaac gaggttgctc caggtgatga
     2821 gctgaacagt ttctctcttt atacaatagg ttcaggaaca aagatgcagt atcttcaggc
     2881 tcacagaggt ctggatgatt catttgaact ttggggcgga aaggttacag gaaagtactg
     2941 gctggcaaca ggcggtctgg atgacgattt tgattttgac gaaggttttc agggaaatct
     3001 gcagtatctt atcggtcata agtatcctac aagctgcgga ggttctcctt caactgatcc
     3061 tcatggattt gaaatggacg ggatcaattc aggaagcggt cagtcagtat gcgattctcc
     3121 taaaagatgt tccaacccga ctatcagtga ctatacagtt c
//
LOCUS       DAOQGJ010000221         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18083_length_3159_cov_3.223260, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000221 DAOQGJ010000000
VERSION     DAOQGJ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18083_length_3159_cov_3.223260"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2420)
                     /locus_tag="PK683_04685"
     CDS             complement(<1..2420)
                     /locus_tag="PK683_04685"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH07775.1"
                     /translation="MNRILKKLNIGAVLFLILCQTLFANPFPKKSAYLEKVFTSTDGL
                     SHNIVSSIVQDKKGYLWIGTYGGLNRYDGFEFRNFNMRDGLAFDAIRSLAATDDGALW
                     IGTEKGLNRFKDEKFELFTVEQSGKGLAGNDISAVRADAKGNLFVGTITGLSVYRNGK
                     FERISLLENDSETVQVNFIHTDAKGLTWVGTKKHGLFILQEDRILKQLKIPQPNEGAN
                     SKSESAELISADSDSNETALIVSTKDKGVFIVIYNNFEAVPLTEYDGGKFYSEDMNGM
                     IICKNPKDRKISFMSQKKKSFIFEDSRYKVLEEKNLQACFVDREGTFWIGTYGLGLVK
                     HYRPRAIGITKEDGLKDLSIRFIFKDSKERLWLGTQNNITQFAQNSVPDEKPFAVRKI
                     YENDSNGNPISKVRWILEDKKGRVWFGTDDRLLFFQNGNLMPAELKDIKKISIYSMCE
                     YMGRIFIATKDKKLYSFDPDTMAFYRHPFPGDMKLGVIWKLTKDHESKVLYIQGSDAV
                     WKYSMDSEGNDSFQLFISKDMHKMYKIQNFEIYSEQDYILSDDSVIIRSRDKDIPAVI
                     GTDQGLPSDQVVSIARTEKGDIWIGTSRGVVRYADGRIVSVYNKADGLVGDFSHFNSI
                     FADSRHVLVGTSEGLSVIDLSDPHKNGIKPESVFTKIVTSGRKQIFELNGTVTLEPED
                     SSIHLFASSLSLSNPEKTRYSFSLSEDSRGSFLKNNTASYYNLSPGKYRFYVRSMNDD
                     GVSSETAGSLEVNVLPAVWQTNWFRGLCVIVLLAVFYSIYRVRVYQMRLQNIRLEGLV
                     RDRTKELQA"
     gene            2528..>3159
                     /locus_tag="PK683_04690"
     CDS             2528..>3159
                     /locus_tag="PK683_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07776.1"
                     /translation="MRTMLILFFFSFCSDIKRPPLYSFLLSGGGTSELAGVISSDFGA
                     GGRFTAVNADLLAASPSYASVHSDAVFRYFKRKVYIVNRLNRDSILILNRDLGFLAEK
                     EFSVGAGTNPQDILAVSDSKAYVPLYGSTDLLTVDLGTGFISKRIYLGNYSESFSSGG
                     APDGKPEMHTMIQYGSSAYLILQRLDRNDLSGFPTPASSSYLLEMDINTDA"
BASE COUNT          838 a          734 c          630 g          957 t
ORIGIN      
        1 gcctgaagtt ccttggttct gtcccttaca agaccttcaa gccttatgtt ctgcagtctc
       61 atctgataca ccctgaccct gtatatggaa tagaaaactg ccagaaggac gatcacgcag
      121 agacccctga accagtttgt ctgccatact gcaggaagaa cattcacttc gagagatccg
      181 gcagtttcgc tagaaacccc gtcatcattc attgatctta catagaatct gtattttcca
      241 ggacttaaat tataataaga tgctgtatta ttcttgagaa aacttcctct cgaatcttca
      301 gaaagagaaa aactgtacct ggttttttca ggattggaga ggctcagaga agatgcaaac
      361 agatgaatgc tgctgtcttc aggttcgagt gtgactgtgc cgttcaactc aaaaatctgc
      421 tttcttcctg aggttacaat cttggtaaag acgctttccg gctttatgcc gttcttatgc
      481 ggatcagaaa gatcaattac agagaggccc tcgcttgtgc ccacgaggac atgccgactg
      541 tcggcaaaga tggaattgaa atgagagaaa tctccgacaa gcccgtccgc cttgttatat
      601 acgcttacaa tccttccgtc cgcataccgc acgactcccc tgcttgtgcc gatccagata
      661 tctccctttt cagttctggc aatgcttacc acctgatccg agggaagtcc ctgatcagtg
      721 ccgattacag ccggaatatc cttgtccctg cttctgataa ttacagaatc atcagaaaga
      781 atatagtcct gttcagagta gatttcaaaa ttctgaatct tgtacatctt gtgcatatcc
      841 ttgctgatga aaagctggaa actgtcattc ccttcagaat ccatgctgta tttccagaca
      901 gcatctgatc cttgaatata aagaacttta ctttcatgat ccttagtaag tttccagatg
      961 actccaagtt tcatatcgcc gggaaaagga tgcctgtaaa aggccatagt atccggatcg
     1021 aatgaataga gtttcttgtc ctttgttgca ataaagattc tccccatata ctcgcacatt
     1081 gagtatatgc tgattttttt aatatccttc agttcagcag gcataagatt accgttctga
     1141 aagaaaagaa gcctgtcatc agtaccaaac cagactcggc cctttttatc ctctaaaatc
     1201 catctgactt tgctgatcgg atttccgttc gaatcatttt cgtaaatttt acggacagca
     1261 aaaggttttt catccgggac actgttctgt gcaaactgtg tgatattgtt ctgtgttcca
     1321 agccagagtc tttcctttga gtccttgaat atgaaacgga tgctcaggtc tttcagaccg
     1381 tcttcctttg tgatgccgat tgccctgggc ctgtagtgtt tgaccagtcc aagcccgtat
     1441 gtgcctatcc agaaagttcc ttcacggtcg acaaaacagg cttgaagatt tttttcttcc
     1501 agcaccttgt acctgctgtc ctcaaaaatg aatgattttt ttttctgaga catgaagctg
     1561 atttttctgt ctttcggatt cttgcagata atcattccgt tcatgtcttc cgaataaaat
     1621 tttccgccgt catattcagt cagcgggact gcttcaaagt tattgtagat cacaatgaaa
     1681 acacccttgt ccttagtaga tacaataagt gccgtttcat ttgaatcact gtccgcagaa
     1741 atcagttcgg ccgattcaga ttttgaattt gctccttcat tcggctgagg aattttcagc
     1801 tgcttgagta tcctgtcttc ctgcagaatg aacagtccat gtttctttgt gccgacccag
     1861 gtcagcccct ttgcatctgt atgaatgaaa ttcacctgaa cagtttcaga atcattttcc
     1921 aggagagaga ttcgttcgaa cttgccgttt ctgtatacag aaagtcctgt aatagtccct
     1981 acaaaaagat ttccttttgc atcggccctg acagcactga tatcatttcc tgcaagtccc
     2041 tttcctgact gctctacggt aaaaagctca aacttttcat ccttgaatct gttcagacct
     2101 ttttccgtcc ctatccagag agcaccgtca tcagttgcag ccagtgaacg gattgcatcg
     2161 aacgcaagtc cgtctctcat attgaaattt ctgaattcaa atccgtcgta tctgttcaaa
     2221 cctccgtaag tccctatcca gagatacccc tttttatcct gaacaatact gctgacaata
     2281 ttgtgagaaa gtccgtcagt ggatgtgaaa actttttcga gatatgcaga ctttttagga
     2341 aaggggtttg cgaaaagggt ctgacataaa atcagaaaca gcaccgcacc gatattcagt
     2401 tttttcaaaa ttctattcat tcaaatatcc cgagagcaga ccgaaatctc agatccgcct
     2461 ataaaaatat cggcaaatcc tctcttttta ttgtgtcgta aaaaactgca tcgcctgaac
     2521 tgtatctgtg agaacaatgc tcattctgtt ttttttcagt ttctgttcag acattaaacg
     2581 gcctccgctg tattcattcc ttctgtccgg aggaggcaca tcagaacttg caggagtcat
     2641 cagttctgat ttcggggcgg gcggaagatt cactgcagtg aatgctgatc ttcttgcggc
     2701 aagtccttcc tatgcttcgg ttcattcaga tgccgtgttc agatatttca aaagaaaggt
     2761 ttatatagtc aacagactga acagagacag catactcata ctcaacaggg atctgggatt
     2821 tctggcagaa aaagaattct cagtaggagc aggaacaaat cctcaggata tccttgcggt
     2881 ctccgattca aaagcatatg tgcccttata tggaagcact gacctgctga ctgtggatct
     2941 gggaacaggt tttatttcca aaaggattta tcttggaaac tattctgaaa gcttcagttc
     3001 gggaggagct cctgacggaa aaccggaaat gcatacgatg attcagtatg gaagcagtgc
     3061 ctatctgatt ctgcagcggc tggacaggaa tgatctttcc ggatttccca ctccggcatc
     3121 ctcttcgtat cttctggaaa tggatatcaa tacggatgc
//
LOCUS       DAOQGJ010000222         3158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18093_length_3158_cov_2.768611, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000222 DAOQGJ010000000
VERSION     DAOQGJ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18093_length_3158_cov_2.768611"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..511)
                     /locus_tag="PK683_04695"
     CDS             complement(14..511)
                     /locus_tag="PK683_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNH07777.1"
                     /translation="MKKTLILSVLFLTAMALTTGISSAPKYGTAGCGIGSLIIKEQGF
                     AQVLAATTNGIYGNQTSGITTGSLGCTADGIVKSEKEQDIFVHMNIESLEQEMAAGKG
                     EKLNTLATLFGCPKDSQRNFANMTQKKYGVLFTNDVRNSPSELISAIKTEISKDSALA
                     SVCKI"
     gene            complement(530..1024)
                     /locus_tag="PK683_04700"
     CDS             complement(530..1024)
                     /locus_tag="PK683_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNH07778.1"
                     /translation="MNRNKFLYLSVALAALTLVNGVSSAPKYGMAGCGLGSIIFTTND
                     KSQILAATTNGTYGNQTFGITSGTSNCTADGIVKNEKAQEVFATVNFGSLEQEMAAGK
                     GEKLNTFAHLLGCSSDSLGRFGQMTQSSYSRLMSENSTPASLIIAVKSEIKKDANLSK
                     ACSI"
     gene            1281..2141
                     /locus_tag="PK683_04705"
                     /pseudo
     CDS             1281..2141
                     /locus_tag="PK683_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
     gene            2302..>3158
                     /locus_tag="PK683_04710"
     CDS             2302..>3158
                     /locus_tag="PK683_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH07779.1"
                     /translation="MRKILFGSVRKLLLLLVFCPFLSTSAFPELVLKGENTVNPIGTA
                     DYTYELSREDSIQNVMHSPVSIWRTSLKEDTFLYNGGEKHLWFRFDLKNESMDSHYFI
                     RVREFALERIICYLIFSDGTIQRHSGGTKFPLKSKTVYSSDSIFPVTLKTGENVRVYI
                     DIQAPIITYSYMEVQSSSSLLDGIIKEHFFLGIYYGLLLFMVILSLATYFYLRDNSYL
                     YFMIFVLLQGLFQMARTSIGTYFLYPDWPERNLDIWYFCLIFSFSIGSLFSRSFLNME
                     IHAKKINRT"
BASE COUNT          877 a          648 c          654 g          979 t
ORIGIN      
        1 aggaaaaagc ggcttagatt ttgcagactg aagcaagtgc gctgtctttt gatatttcgg
       61 tcttgattgc tgaaatcaat tctgacggag agtttcttac atcatttgta aaaagtactc
      121 cgtatttttt ttgagtcata ttcgcaaagt ttctttgaga atcctttgga catccgaaaa
      181 gagtagcaag tgtgttcagc ttttcacctt ttccggcagc catttcctgt tccagacttt
      241 caatgttcat atgaacaaag atatcctgtt ctttttcaga ttttacaatc ccgtcagcag
      301 tacatccgag agaacctgtt gtgattcctg aagtctgatt tccataaatc ccgtttgttg
      361 tagcagcaag aacctgtgca aaaccttgct ccttgatgat taaggatccg attccgcatc
      421 cggcggtgcc gtatttagga gcactggaaa tcccagtagt cagagccatt gcagtaagaa
      481 agaggacgga taaaattagt gtttttttca tgaatgatct cctggaaaat taaatgctgc
      541 aagctttgga aaggtttgca tcttttttga tttctgattt gactgcaata atcagagaag
      601 caggggtgga attttctgac atcagtctgc tgtagcttga ctgagtcatc tgtccgaatc
      661 ttcccagaga atccgaactg catcccaaaa gatgagcaaa ggtattcagt ttttctcctt
      721 tacctgcggc catttcctgt tcgagacttc cgaaattcac agtggcaaaa acttcctgtg
      781 ctttttcgtt tttcacaatc ccgtctgctg tacaattaga tgttccggaa gtgattccaa
      841 atgtctgatt accgtatgtt ccatttgtgg ttgctgcaag gatctgcgat ttgtcattag
      901 ttgtgaagat tattgatccc agtccgcaac ctgccattcc atactttggt gcacttgaaa
      961 ctccgttgac aagagttaat gctgccaaag caactgataa atatagaaat ttgtttctat
     1021 tcataaactg ctccatgatt gaaagtattg ctcatctttg acagattgga acttgagtca
     1081 ataaaaaaaa tgttttattt aaaaaagtca tttcagatga atttgatttg ttcgtctaaa
     1141 aaagaggatt tactgtgctg cctttgaaat tcagaatccc gctgaatcag atattcagag
     1201 atatgtcaat aatccgcatt tttcacattc cttcccgttt tactgtttta catgaaaaca
     1261 cctcaattta ttcagtactt atgaagctga agaatgagtt tagtgtcgga aaatatacga
     1321 taggggaaag ttcgagacct ttcctggcgg ctgaaatcgg actcaatcac aataatgatc
     1381 ccgaaatagg aaaaagaaca attgccgccg ctgcaaaggc aggagctgat gcagttaaat
     1441 tccagtccta cgtgactcat gaatttattg acagcaggaa ttcagaagcg aaattccttt
     1501 ttgatatatt caaaaagtat gagctttccg agaaactgca cagggaattt cagaagactg
     1561 cagagtctga aggagtgatt ttcttttcca ctcctctctg tgaaggctct gtggatcttc
     1621 tgctttcaat gaatgtacct gtcatcaaaa tagcctccgg ggacattgta aattatcccc
     1681 ttctcaaaaa aactgcagaa acaggtctgc cagtatttct ctcgagcggt gccgccgatt
     1741 tttatgaagt gacaagagct atggaatttc tttctgcttc tgtggacaga atctgcctta
     1801 tgcactgtct ttcgatgtat ccggctcctc ctgaaaagct caatctcaga acactggaac
     1861 tttataaaga aatttacgaa tgtccgcttg gcttttcaga tcattctgcc gggacactcg
     1921 gagctgccct ttctgtcggc ctcggtgcat ctgtaattga aaaacatttt actcttgata
     1981 aaaatcttcc tgggcccgac catcagattt ctgcatctcc cgacgaactg aaaaaacttc
     2041 gggaagacat tgacacggct ttcagaatgc gcggagaaaa agtcaaacgt ccgactgcgg
     2101 aggaagcggc gggaaggttt tacgggcgca gatctgcata tgtccaaaat tcacgcctga
     2161 ctgcacttcg gcctgctctt catgcgaggg attctgcaat tgccgattcc tgggatataa
     2221 aagatatgca aaataatgaa aaagcgtctg aaggggcggt tcgtaaattt accgtctgac
     2281 tgcagattcc aagtgcacag tatgagaaaa attctcttcg gttcagtcag aaagcttctg
     2341 ctccttctgg ttttctgtcc tttcctcagt acctcagctt ttcctgaact cgtattgaag
     2401 ggggagaaca ctgtaaaccc gataggtaca gctgactata catacgaact cagccgagaa
     2461 gattcaatcc agaacgtaat gcattctccg gtaagtatct ggagaacctc gcttaaggag
     2521 gatacatttc tctacaacgg aggtgaaaag catctctggt tcagattcga tctgaaaaac
     2581 gagagcatgg acagtcatta tttcatccgt gtaagagagt ttgcactgga aaggattata
     2641 tgttatctta tattttctga cggtacgatt cagagacatt cgggggggac aaaatttcct
     2701 ctcaaaagca agactgtata ttcatcggac tctatttttc cagtcactct gaagacgggt
     2761 gaaaatgtaa gagtttacat agacatacag gctcctatca taacttattc ctatatggaa
     2821 gtccagtcaa gcagcagtct tctggacggc atcatcaagg agcatttctt tctgggcatt
     2881 tattacgggc ttctgctttt tatggtcatt ctgagtcttg ccacgtattt ttatctgaga
     2941 gacaattctt atctttattt tatgatcttt gttcttctgc agggactttt tcagatggcg
     3001 aggacttcga taggaaccta ttttctttat cctgactggc cggagagaaa tctggatata
     3061 tggtatttct gtctgatttt cagtttctcc ataggatctc ttttctccag aagttttctg
     3121 aatatggaaa tacacgctaa aaagataaac agaacaat
//
LOCUS       DAOQGJ010000223         3155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18120_length_3155_cov_2.783871, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000223 DAOQGJ010000000
VERSION     DAOQGJ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18120_length_3155_cov_2.783871"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PK683_04715"
     CDS             <1..647
                     /locus_tag="PK683_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNH07780.1"
                     /translation="EGNILEYPFLGLLLSGGNSAVFKVDEPGKMTVLADTRDDALGEA
                     LDKAAILLGLGYPGGPLIEKKAAEFQGERKGALFPKVNTGGNNRFFSYSGLKTSLLYF
                     TRKNPDYISRIPEVCWHYQNSAFELVEKNISKLVSDTGIHRVVAAGGVLANTFLRERL
                     EKLSVKKRFQLIYPKKKLLCTDNGAMTACLGYYLFQAKKISGLDFAVSPYREET"
     gene            644..1402
                     /locus_tag="PK683_04720"
     CDS             644..1402
                     /locus_tag="PK683_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH07781.1"
                     /translation="MKLKLNWVPNTLSLGNLTFGFISILVSSSAVIGSPENSKLFFLS
                     GVFIILAAVFDGFDGMVARALNATSDLGAELDTLADLTTFGIAPGFLMYKMILQKYQL
                     DLFNTGNYFHVGMFIAALFPICAAYRLARFTVAHDPSSFTGLPSPIAGVIVAFFPITF
                     PNSQIADILTVTGFVVIAWLMVSTIRYSKPQVAMRGKFSKGKLIFMVGSLVAVLAGVA
                     VGAPKLLPYLFYGMILFYVFSGIISLFIQIIQEF"
     gene            1403..2695
                     /locus_tag="PK683_04725"
     CDS             1403..2695
                     /locus_tag="PK683_04725"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH07782.1"
                     /translation="MRGFFYKTLNKYLQVFIRPEDSLLVVDPEDEKLFSEHSHKDIVH
                     HRTGESSLSSVKNKAYSYILLNGNVHYERDIETFLNSIHSVCSSETRVAVTYFSTLWK
                     PVLRLATFLRLRRKTEESNWVTHEDIANLLLLSDFEIIRREGKILFPIYIPLISHFLN
                     RYLAPLPFFRNFTLVNILIARPLFQDEKRKKLSVSVVVPARNESGNIEKIIERLPKMG
                     PDDELIFVEGNSTDDTWQKIQEYAAKYKNEKNIRIFQQEGKGKGDAVRKGFSNAEKDI
                     LMILDADMTVPPEDLPRFYSAIVKGKGEFINGSRLVYPMEKEAMRFFNLLGNKFFAMA
                     FSFVLGQRFKDTLCGTKVLTRENYILLSKNRSYFGEFDPFGDFDLIFGAARMGLKIVE
                     IPIIYRERTYGTTNISRWKHGAILLGMLIFAARKIKFI"
     gene            2927..>3155
                     /locus_tag="PK683_04730"
     CDS             2927..>3155
                     /locus_tag="PK683_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH07783.1"
                     /translation="MLKEKIAEREKSESVQTEEEVQFLSFTVSNELFGIDLIHVHEIL
                     KPAFITRIPNVEDFVLGVINLRGEIIPIVDLK"
BASE COUNT          913 a          600 c          704 g          938 t
ORIGIN      
        1 tggaaggaaa tattctggag tatcctttcc ttggcctgct tctgtcagga ggaaattctg
       61 ctgtattcaa agtggatgaa cctgggaaaa tgacagttct ggcagatacc agggatgacg
      121 ctttgggcga ggctttggac aaggcagcaa tcctgcttgg tcttggatac ccgggaggac
      181 ctctcattga aaaaaaggcc gcagaatttc agggtgagag aaaaggtgcc ctttttccca
      241 aagtaaatac aggaggaaat aaccgtttct tttcctattc aggcctgaaa accagtcttc
      301 tctattttac cagaaaaaat cctgattaca tcagcagaat tcctgaagta tgctggcact
      361 atcaaaattc tgcatttgaa cttgtagaaa aaaacatatc aaaactcgtg tcggataccg
      421 gaattcaccg ggtagtcgca gcaggaggtg ttcttgccaa tactttcctg cgggaacggc
      481 tggaaaaact ttccgtgaaa aagagatttc aattgattta tccgaaaaaa aaattgcttt
      541 gtactgataa cggggcaatg acagcctgtc tggggtatta tttatttcag gccaaaaaaa
      601 tttcagggct ggattttgca gtcagtcctt acagagagga aacatgaaac taaagttaaa
      661 ctgggtaccg aatacacttt ctttgggaaa tcttacattc gggttcattt ctatccttgt
      721 atcttcgagc gcggtgatcg ggagtccaga aaattctaaa ctgttttttc tttccggcgt
      781 ttttatcatt ctggcggccg tttttgacgg atttgacggg atggtggcca gagcgctgaa
      841 tgctacaagt gaccttggtg ctgaactgga cactcttgcc gatctgacca cattcggaat
      901 tgctcccgga tttctgatgt ataaaatgat tctgcagaag tatcagctcg atctttttaa
      961 tacaggaaat tattttcacg tagggatgtt tattgccgct ctttttccga tctgtgccgc
     1021 atacagactt gccagattca cagttgcaca tgatccttct tcatttaccg gtctgccttc
     1081 accgatagcc ggagtaattg tggctttttt cccgattacc tttcctaaca gtcagattgc
     1141 cgacattctt actgttacag gatttgtggt cattgcctgg ctgatggttt ccacaatcag
     1201 atattccaaa cctcaggtgg caatgagagg gaagttttcc aaggggaaac ttatttttat
     1261 ggtagggtcc ttggttgcgg ttcttgccgg tgtggcggta ggggctccca aacttctgcc
     1321 ttatctgttt tatggaatga tactgtttta cgttttttcc ggaatcatca gtcttttcat
     1381 tcaaatcatt caggaatttt aaatgagagg ttttttttat aagactctga acaaatatct
     1441 gcaggtattc attcgtcctg aagacagtct tcttgtcgtt gatccggaag atgaaaagct
     1501 tttttctgaa cattcccata aagatattgt gcaccacagg acaggtgaaa gcagtctttc
     1561 ttcagtaaaa aataaagcat attcctatat tttactgaat ggaaatgttc actatgaaag
     1621 agacattgaa acttttctga acagtatcca ttcagtgtgc agttcagaaa caagagtggc
     1681 cgtgacttat ttttccactc tttggaagcc cgtactgcgt ctggccacat ttctgcgact
     1741 gagaagaaaa acagaagaat caaactgggt gacacatgaa gacattgcaa atcttctcct
     1801 gctttctgat ttcgaaataa tccgaaggga gggtaaaatc ctgtttccca tttatattcc
     1861 gctcatcagc cattttctga acaggtattt ggcccctctg ccttttttta gaaattttac
     1921 cctggtgaat atactcattg cgagaccttt atttcaggac gagaaaagga aaaagctgag
     1981 tgtatcagtg gttgtgccag ccagaaatga atcagggaac attgaaaaga ttattgagcg
     2041 actccctaaa atgggacccg atgatgaact gattttcgtg gaaggaaatt ctactgatga
     2101 cacctggcag aaaattcagg aatacgccgc caaatacaaa aatgaaaaga atatccgaat
     2161 ttttcagcag gagggaaaag ggaaaggcga cgctgtccga aaaggctttt caaacgcgga
     2221 aaaggatatt ctgatgattc tggatgcgga tatgacagtt cctccagaag atcttcctcg
     2281 tttttacagt gcaattgtga aaggaaaagg cgaattcatc aacgggagcc gcctagtata
     2341 tccaatggaa aaggaagcaa tgcggttttt caatctcttg ggaaataaat tctttgcaat
     2401 ggcattttcc tttgttttag gtcagaggtt caaggatact ctctgcggaa caaaagtgct
     2461 tacccgtgaa aactatatcc tgctatccaa aaatagaagt tattttgggg aatttgatcc
     2521 attcggagat tttgacctga ttttcggggc tgcaagaatg ggactgaaaa tagtggaaat
     2581 tcccattatt taccgagagc gcacttacgg aactacgaat atttccagat ggaaacacgg
     2641 ggcaattctg cttgggatgc tgatttttgc agcgagaaaa attaaattta tttaatgaaa
     2701 ttccctgtgt aaggcaaaag aaaacggatc actcgaagaa gcggacagga atgctgtttt
     2761 ttgcttcttc gaaatcttct ttcagttcct gggttgggcg gattcgattt tatatcgagt
     2821 tcaccgtaaa atccacgatt ttatttgcca aaagaaaggt gttttccgat agtataaaaa
     2881 gggtatgttt tcagaaaata cagcaaaaca ggcgatgctt cagaatatgc tgaaggaaaa
     2941 aattgccgaa agagaaaaat ccgaatctgt tcagacagag gaagaagtgc agttcctgtc
     3001 ctttacagtt tccaacgaac tgtttggcat tgacctgatt catgtgcatg aaatactgaa
     3061 gccggcattt attactagaa ttccgaatgt agaagatttt gtactcggag tcatcaattt
     3121 gagaggggaa attatcccta tcgttgatct gaaaa
//
LOCUS       DAOQGJ010000224         3147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18188_length_3147_cov_3.546572, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000224 DAOQGJ010000000
VERSION     DAOQGJ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18188_length_3147_cov_3.546572"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PK683_04735"
     CDS             <1..610
                     /locus_tag="PK683_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNH07784.1"
                     /translation="FEYELSLDLEHSQQSEKLKEWLNIIVRCNEPTILEEASSQVLLD
                     IAKNNYRFMDGNVQPLLTADELNYLSIKKGSLDFVERKEIESHVEHTFQFLSKIPWTP
                     GLKMVPSIAHAHHEKLDGTGYPRGLVSFEIPVQSKMMTISDIFDALTDKDRPYKKAVP
                     LERALDILKMEVKDEHVDGELLNIFIEAKVYERLSGKSVQPF"
     gene            684..1727
                     /locus_tag="PK683_04740"
     CDS             684..1727
                     /locus_tag="PK683_04740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNH07785.1"
                     /translation="MLEHYEKKIYDQKQLIEISKALNSTLDYENLKDSLLNTCLAQLQ
                     TMNAALILAPDVDANYIRLDKNYKGFDISQQELDMKILLDSPLIQVFEEKFKAMTIRN
                     LEQHHSYNPVGDEYFRFFKNLGVEMIAPLNAKGKINGLLILGEKMTMSEYLENDKDFL
                     TVLASLSGIAVENIRLYELATVDMMTQLKVHHYFQTRLREEMDRCRKKGSKLSLLFTD
                     VDKFKVFNDTYGHQAGDVVLIEVAKKVMECCRRNDVAARYGGEEFCVVMPGATIEDGF
                     EMGEKIRKSIEGHRVVNPASGEEVKVTISVGVTEFHPADRNNKELIERSDQALYKAKH
                     GGRNQTVINPRLD"
     gene            1908..>3147
                     /locus_tag="PK683_04745"
     CDS             1908..>3147
                     /locus_tag="PK683_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH07786.1"
                     /translation="MRLLISFLFIFGLFTSPVFSAKKYALIIGSNYKGNKSGIGELQL
                     CEADATYMESQIRKVGNFDEIKVLLGRDVTKTNIEKEIRSIGDKASKDDSVFLYFAGH
                     GFYQRDASAKNGMRNFIVCYDRPHLSDDELNEYLKSIKSPKTLFAFDCCYSGGIAKKG
                     KQTRGDKAIPIPEGSQGTVKTDDDFFFQNKAMISSADDNQVAIEVGGTINHGIFTYHF
                     GKAMETADLNGDKVVTALEAFFKTRDDVGRTARENDHVQTPQISGDASGFFLAGADRP
                     EPPKVDPTPVVKPEPKPEPEYNPPKKDPDNPPVTPAEPPAVQTSKKGGLLIRTSIIKD
                     RKFGWVNLTPEQMIKNRERKGTRAVKVLIDDQDVNYNLITVKSDNWGATTTSKGEVYN
                     ISMKNIPAGVHKLTVRADDYP"
BASE COUNT         1008 a          597 c          719 g          823 t
ORIGIN      
        1 ttttgaatat gaactctctt tggatctgga acactcccag cagtctgaaa aactgaaaga
       61 atggctgaat attatcgtcc gatgcaatga gcctacaatt ctggaggaag cgagttcaca
      121 ggttcttctg gacattgcaa aaaataatta cagatttatg gatggaaatg ttcagcctct
      181 tctgactgcc gatgagctta attatctttc catcaaaaaa ggatcactcg attttgtaga
      241 acgcaaggaa atcgaatctc atgttgagca tacttttcag tttttaagca aaatcccttg
      301 gacccccgga ctgaaaatgg ttccaagcat tgcacatgct caccatgaaa aattggatgg
      361 aaccggttat cccagagggc ttgtttcctt tgagattcct gtccagtcaa aaatgatgac
      421 tatctccgat attttcgatg ctctgacaga caaggaccgt ccttataaaa aagcagtacc
      481 tctcgaaaga gctctggata ttctgaagat ggaagtcaag gacgagcatg tagacggaga
      541 acttctgaat atttttattg aagccaaagt gtatgagaga ttaagtggaa aatctgttca
      601 gccgttctaa tcaataatat tttatctgat aaaaggcaag ggggtttatt ttgaaaaaac
      661 aggaagaaag cagtactttt gaaatgctcg agcattatga aaagaagatt tatgatcaga
      721 agcagctcat agaaatcagc aaggcactga attcaactct tgactatgaa aacctcaaag
      781 attcactttt gaatacatgc cttgcacagc ttcagacaat gaacgcagca cttattctcg
      841 cacccgatgt ggatgcaaat tatatccgtc tggataaaaa ttacaagggt ttcgatatca
      901 gccagcagga actggatatg aaaattcttt tggattcacc gctgattcag gtgtttgaag
      961 aaaaattcaa agcaatgaca attcgaaatt tggaacagca tcattcgtac aatccagtag
     1021 gagacgagta ctttcgcttc tttaaaaatc tgggagtcga aatgattgct cctttgaatg
     1081 cgaagggaaa aatcaacggt cttctgattc tgggtgagaa gatgactatg agcgaatatc
     1141 tggaaaatga caaggatttc cttacagttc tagcaagtct ttccggaatt gcagtggaaa
     1201 atatccgtct gtatgaactg gcaactgtgg atatgatgac tcaattgaaa gtccaccatt
     1261 attttcagac taggcttaga gaagaaatgg acagatgcag aaagaaaggt tcaaaacttt
     1321 cactactgtt tactgatgtg gacaagttca aggtattcaa tgatacttac ggtcatcagg
     1381 caggagacgt tgtactgatt gaagtagcca aaaaggtaat ggaatgctgc cgcagaaatg
     1441 atgtggctgc cagatacggc ggggaagaat tctgcgttgt aatgccaggt gctacaattg
     1501 aggacgggtt cgaaatgggt gaaaaaatcc gtaaatcgat tgaagggcac agagtcgtca
     1561 atcctgcatc aggagaagaa gtgaaagtca ctatcagtgt cggagtgaca gaatttcatc
     1621 ctgccgacag aaacaacaag gaactgattg aaagatccga tcaggcactg tacaaggcaa
     1681 aacacggggg cagaaatcag actgtcatca atcccaggct tgattaatcc gtcgaaacaa
     1741 gttttatatt gaacatttcg gcagttccca aaatctaatg atagacgggt gtaggaaatg
     1801 ttcagtggaa gattactgct tactctgatc cacaattcag acatcagttt tttctgcagg
     1861 ggctgttttc tgcgggacgt tctatctaag tattagggag gtaaggtttg aggctgctca
     1921 tatcatttct ttttattttc gggcttttta cgagtccggt tttctctgcc aaaaaatatg
     1981 cattgatcat tggttccaat tacaagggaa acaaatcagg catcggtgaa cttcagctct
     2041 gcgaagcgga tgcgacttat atggagtctc agatcaggaa agtcgggaat tttgatgaaa
     2101 tcaaggttct cctaggcaga gatgtcacca agacaaatat agaaaaagag attcgttcca
     2161 ttggagacaa ggcatccaag gatgattctg tttttcttta ttttgccggt catggttttt
     2221 atcagagaga cgcaagtgcc aaaaacggga tgcgaaattt catagtatgt tatgacaggc
     2281 ctcacctttc ggatgatgaa ctgaatgaat atctgaaatc aatcaagtcc cctaaaactc
     2341 tgtttgcatt tgactgctgc tattccgggg gaattgccaa gaaaggaaaa cagaccaggg
     2401 gagacaaggc aatcccgata ccggaaggat ctcagggaac agtgaaaacc gacgatgatt
     2461 tcttctttca gaacaaggca atgatttctt ctgccgatga caatcaggta gctatagaag
     2521 tgggcggtac aatcaatcac gggattttta catatcattt tggaaaggca atggaaacgg
     2581 cagatctgaa cggagacaaa gttgtcactg cactggaggc tttttttaag acaagagacg
     2641 atgtaggaag aacggccagg gaaaatgacc atgtgcagac acctcagatt tcaggtgatg
     2701 cttcaggatt ctttcttgca ggtgcagaca ggccggaacc tccaaaagtg gatccgactc
     2761 cagttgtaaa acctgaacct aaaccggagc ctgagtacaa tcctccaaaa aaagatcctg
     2821 ataatccccc cgtcactcct gcagaacctc ctgcagttca gacatccaaa aaaggaggac
     2881 ttctcattcg tacatccatt atcaaggaca gaaaattcgg atgggtaaat cttactcctg
     2941 agcagatgat taagaacaga gagagaaagg gaaccagggc agtaaaggta ctgattgatg
     3001 accaggatgt gaattataat ttaattacag taaaatcgga caactggggt gcaactacga
     3061 catccaaggg tgaagtctac aatatttcca tgaaaaatat tccggccgga gtccataagc
     3121 tcacagtgcg tgctgacgat tatcctg
//
LOCUS       DAOQGJ010000225         3134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18286_length_3134_cov_3.434232, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000225 DAOQGJ010000000
VERSION     DAOQGJ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18286_length_3134_cov_3.434232"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK683_04750"
     CDS             <1..817
                     /locus_tag="PK683_04750"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNH07787.1"
                     /translation="VLPVGAKTFAEALRMGAEVFHTLKTVLKGKGYNTAVGDEGGFAP
                     DLKSNQEGFEVILEAITKAGFKPGKDILLGLDAAASELYSKEKKKYVMGKKSKNKADI
                     SEKTSEEMIEYYAKCVKDYPLITIEDGLDENDWTGWKALSEQLRDKVQLVGDDLFVTN
                     VGKLKTGIEKKIGNSILIKVNQIGTLTETLAAIEMAQKAGYTAVVSHRSGETEDTTIS
                     HIAVAMNAGQIKTGSLCRTDRVAKYNELLRIEEELGSSAVYKGKAAFYNVNVA"
     gene            807..1238
                     /locus_tag="PK683_04755"
     CDS             807..1238
                     /locus_tag="PK683_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07788.1"
                     /translation="MWLKDRTKILFAFGIVFALMYSTILGGSGVIERARLESKLQALS
                     DDVIRLEAENTRLEKKKFNPSQDVIMNPEYSYVIKFKEFIEFNGVDGPLATHIPLSRM
                     ENKQARNFISNMNLVKSLYLIAGGIFAAAFIYRRKMQRARA"
     gene            1249..1614
                     /locus_tag="PK683_04760"
     CDS             1249..1614
                     /locus_tag="PK683_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07789.1"
                     /translation="MSSGNLEEQILFLETVFLILFSILPSIVISILIKKIFNSEQTKI
                     QLLKMVPIVSFSSSVLFILVSFATMMAILLNYDQFTCILTAFSVYTIGFLVLSYSAMI
                     LSITDGKIRKSEPKYSIES"
     gene            1794..2324
                     /gene="flgN"
                     /locus_tag="PK683_04765"
     CDS             1794..2324
                     /gene="flgN"
                     /locus_tag="PK683_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HNH07790.1"
                     /translation="MLQMQEETQNNLEYILKEEISVYKSLLLLESNKKDSILQSKGKD
                     LEAATKQISHLLSIAVEIESRRKNAMSKVFTEKKISPKSESITLTDFLENLEEEEKPK
                     YISLADDLRKVVTDLREKVEVNEKLLKAKQEIFQLSMEALKVASAGVQEVGYDGYDSG
                     LNTSKPRTNVVINTKA"
     gene            2333..>3134
                     /gene="flgK"
                     /locus_tag="PK683_04770"
     CDS             2333..>3134
                     /gene="flgK"
                     /locus_tag="PK683_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNH07791.1"
                     /translation="MGSTFSGIELSKRGLAAQQQGIHTTGHNISNADNKSYARQRVNI
                     GTNDPIYEPSLNRAMIAGQIGQGASVTEVERIRDMFLDDRIAETNTEKNYWGARQEYL
                     HQVEVIFGEPGGVTLRNHMDQFWSSLEELSKYPEESAQRSVVKEKAIGLSSRIEDTFR
                     KLSVLKDQADKEVEVKTNLLNGLSEQVRVLNERITKAEAMGDNPNDLYDRREKIVEEL
                     SGLVDLSIGRSDRDEFMIFIGQQILVQGSKKNEIIMKGNAEYEGKQDLY"
BASE COUNT         1039 a          581 c          735 g          779 t
ORIGIN      
        1 ggttcttcct gtaggagcta aaacattcgc tgaagcactc agaatgggag cggaggtttt
       61 tcatactctg aaaactgttc tgaaaggaaa aggctacaat actgcagttg gtgatgaagg
      121 cggttttgca cctgatctga agagcaacca ggaaggattt gaagtcattt tggaagcaat
      181 cacaaaagct ggattcaagc ctggaaagga tattctgctt ggtcttgacg ctgcagcaag
      241 tgaactgtac tccaaggaaa agaaaaaata tgtgatgggg aaaaagtcca aaaacaaggc
      301 tgatatttcc gaaaaaacat ctgaagagat gattgaatat tatgcaaaat gcgtcaagga
      361 ctaccctctg atcacaatag aagacggtct ggacgaaaat gattggactg gctggaaggc
      421 actcagcgaa cagctgagag acaaggttca gctggtcggt gatgatcttt ttgtaacaaa
      481 tgtgggcaag ctgaagactg gaatcgagaa aaaaatcgga aattccatcc tcatcaaggt
      541 caatcagatc ggaactttga ctgaaacact tgcggcaatt gaaatggcac agaaggcagg
      601 ctacactgcc gtggtgagtc acagaagcgg tgagacagaa gatactacca tctctcatat
      661 tgctgttgca atgaatgcag gtcagataaa aactggttca ctgtgcagaa cagacagagt
      721 tgcgaaatac aacgaacttc tcagaattga ggaagaactc ggttcatctg cagtttataa
      781 aggaaaggcg gctttttaca acgtcaatgt ggcttaagga cagaaccaaa attctatttg
      841 ccttcggaat cgtgtttgca ctgatgtaca gcacgattct aggcggcagc ggagtgatag
      901 aaagagcgcg tctggagtct aaactccagg cgctctcgga tgatgtcatc cgtctggaag
      961 cagaaaatac cagattagaa aagaaaaaat tcaatccatc tcaggatgtg ataatgaatc
     1021 ctgaatattc ctatgtcatc aaattcaaag aattcattga attcaacgga gtggatggac
     1081 ctcttgccac tcatatacca ttgtccagaa tggaaaataa acaggctaga aatttcatct
     1141 caaatatgaa tttggtgaaa tcactgtatc tgatcgcagg cggaattttt gcagcagcat
     1201 tcatttacag aaggaaaatg cagagagcga gagcctgaaa aatcaggaat gtcgagcgga
     1261 aatcttgaag aacagatcct attcctggag actgtatttc tgatcttatt ttctatactg
     1321 ccgtcgattg ttatatctat tctcatcaaa aaaatcttca attctgaaca gacaaaaata
     1381 cagttgctga aaatggtgcc gatcgtttca ttttccagtt ccgtgctgtt tattcttgta
     1441 tcttttgcga caatgatggc aattcttctg aattacgatc agtttacctg cattctcaca
     1501 gctttttctg tttataccat tggttttctt gtgttgtctt attctgcaat gattctttct
     1561 attacagatg ggaaaattcg aaaatctgaa ccaaaatact cgatagaatc ctaaaaatgc
     1621 cgatgttaaa taaggataaa gtgtataaca gcgttgtgct gactgctcca aagatcggat
     1681 aaactctgga tagggaaaat gatgatcttc cgggcagata tgactttttt ccgctttttt
     1741 caaaagcaaa cactgaattt gcgaaaaccg aattcacgtt gaggagaaga tttatgcttc
     1801 agatgcagga agaaacacag aacaatctgg aatacattct gaaagaagag atttcagttt
     1861 acaaaagcct tctgcttttg gaatccaaca agaaggacag catcctgcag tcaaagggca
     1921 aagatctgga ggcggcgaca aaacagatca gccatcttct ctccattgca gtggaaatag
     1981 agtccagaag aaaaaatgca atgagcaagg tttttacaga aaagaaaatc agtccaaaat
     2041 cagaatccat tacactgact gattttttag aaaacctcga agaagaggaa aaaccgaaat
     2101 atatcagtct cgcagacgac ctcaggaaag tcgtcactga tctgcgggaa aaagtggaag
     2161 tgaatgaaaa acttctgaaa gctaagcagg aaatttttca gctttccatg gaagcactga
     2221 aagtggcttc cgcaggagtg caggaagtag gttatgacgg atatgattcc ggactcaaca
     2281 cttccaaacc ccgtacaaat gtagtcatca atacaaaggc ttaggagaaa aaatgggatc
     2341 cacattttcc ggtatagaac tgagcaaaag aggccttgcg gcacagcagc agggcattca
     2401 tacaacaggt cataatattt ccaatgcaga caataaatcg tatgcaagac agagagtaaa
     2461 tatcggtaca aatgatccga tttatgaacc ttctctgaac cgtgcgatga tcgcaggtca
     2521 gatcgggcag ggggcaagcg tcactgaggt ggaaagaatc cgtgatatgt ttctcgatga
     2581 cagaattgcg gaaacgaata ctgaaaagaa ctactggggt gcaagacagg aatacctgca
     2641 tcaggtggaa gtgattttcg gcgagcctgg cggagttaca ctgagaaatc atatggatca
     2701 gttctggtct tcactggaag aactctccaa gtatcctgag gaaagcgctc agcgttctgt
     2761 agtgaaagaa aaagcaatcg gcctttcctc acggattgaa gacactttca gaaaactttc
     2821 tgtgctcaag gatcaggcag acaaggaagt ggaagtcaag acaaatcttc tgaacggtct
     2881 ctctgaacag gtgagggttc tgaatgaacg gattaccaag gcggaagcga tgggagacaa
     2941 tccgaacgat ctttacgaca gaagggaaaa gatcgtggaa gagctttccg ggcttgtgga
     3001 tctttccata ggaagaagtg acagggatga gtttatgatt ttcatcggtc agcagattct
     3061 cgttcagggt tccaaaaaaa atgaaattat aatgaaagga aatgctgagt acgaaggaaa
     3121 gcaggatctg tact
//
LOCUS       DAOQGJ010000226         3132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18309_length_3132_cov_3.318167, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000226 DAOQGJ010000000
VERSION     DAOQGJ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18309_length_3132_cov_3.318167"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK683_04775"
     CDS             <1..191
                     /locus_tag="PK683_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07792.1"
                     /translation="IEERGGVREIFESLEQLKKIHRCFLEVLPKKTEFLNAELPADEL
                     LETAIGRIRFLMNQSAGS"
     gene            complement(157..528)
                     /locus_tag="PK683_04780"
     CDS             complement(157..528)
                     /locus_tag="PK683_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH07793.1"
                     /translation="MTEMNSSQQDNIDSLPIMNRKDALDRLDNNTVLLDKIISMFMEK
                     TPNELVKIEKLLEENNIDQIGFVAHTIKGTSAMIGADRFSACALKIERLAKQNSPKAE
                     IEEKFQMLKQIMNLLTDSLKT"
     gene            848..1177
                     /locus_tag="PK683_04785"
     CDS             848..1177
                     /locus_tag="PK683_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07794.1"
                     /translation="MLKKFLIILVLGTIANCKGISSTKSFSSYDVNSKGNRVRKTFTG
                     PMVVFNSIQFGESIDTKDKLHELLKINECKDLKNIEVEFYNDYILVAGYQKMVISADC
                     SREEAAK"
     gene            1325..2470
                     /gene="galK"
                     /locus_tag="PK683_04790"
     CDS             1325..2470
                     /gene="galK"
                     /locus_tag="PK683_04790"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNH07795.1"
                     /translation="MNESLQSVLLSKFGENQEKITEFQSPARINIIGEHVDYLGGLVL
                     PASINFFTKIAIRPNWDHIFRIYSVQFNETVETSQILRRKTNTWANYILGVISEFIKE
                     GYIIPGFDLVVDGNVPQGAGLSSSASLEVCVGYALSSVFHLPVSRTEIAVFAQRAENN
                     FTGTRCGIMDQFAVSLGKKDKCILLNTDTLEYHYKSVHLGESELYLINSNVKHSLETS
                     AYNDRRKDCENALIKIKTKFLGLKTLYQFTETDRMDLFGFTPNEHKRVLHTAGERKRT
                     AEIMKCFEEGDIERAGEILYQTHFSLSENFEVSCPETDFLVEQLRNLKVQGARMVGGG
                     FGGCILVLDKKGRYENVSAEIQKLYKEKFGINPDFYRFEICDGVGEP"
     gene            2476..2880
                     /locus_tag="PK683_04795"
     CDS             2476..2880
                     /locus_tag="PK683_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH07796.1"
                     /translation="MSDKMDLSSADLAYESSEIKIKVNRNHPDLPEEGVIVDMSGDLN
                     LYSTPNLKTIITKLMDGGYTKFFVNMESLKYIDSSGLGAFLSFQSKLLKMQGFIRICS
                     PSKQVSAVLELTKLKSMLRVSRTVDDAIFNRT"
     gene            2877..>3132
                     /locus_tag="PK683_04800"
     CDS             2877..>3132
                     /locus_tag="PK683_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNH07797.1"
                     /translation="MKEYRTLAEFWPFYLSEHSNIWNRRLHFIGSTCALLSIFSVFYF
                     QSALFLLLAPVSGYGFAWFGHFIIEKNRPATFKYPVKSFVS"
BASE COUNT          933 a          579 c          645 g          975 t
ORIGIN      
        1 ggattgaaga aagaggagga gtcagggaga tttttgaaag cctggagcag ctgaaaaaaa
       61 ttcaccgatg ctttctggaa gttctgccga aaaaaacgga atttctgaat gcagaactcc
      121 ctgcagatga acttctggaa acggcaatag gcagaatcag gtttttaatg aatcagtcag
      181 caggttcatg atctgcttaa gcatctgaaa cttttcttca atttctgcct tgggagaatt
      241 ctgttttgcc agtctttcaa tttttaatgc acaggcggaa aatctgtctg caccgatcat
      301 tgcagaagtc cctttgatcg tatgagctac aaagcctatc tgatcaatat tgttttcctc
      361 taaaagtttt tctattttta ccagttcgtt tggagttttt tccataaaca tagaaataat
      421 tttatccaaa agaacagtgt tattgtccag acggtccagt gcatcctttc tgttcataat
      481 tggcaaagag tcaatattat cctgctgtga actgttcatt tctgtcattt taagtccttg
      541 ttatatagag catactgcct cgactcaagt ttttgaaagg attgatgcgt ttctatgtaa
      601 aagtatatag actttttgga ctggggaaaa acaagcaatt tttaggaata aaaaggattc
      661 attgcagaat cgggtctacg cctcagcttc agaagccttc agagtaattt tcttattttt
      721 gattttccag agggagaccg catccttttc cctgcttgtg agtttcgaaa tctgagtaaa
      781 taatattgac attttccctc aatcttaaac tctgatttta tccaaccgtt aaaagaggga
      841 gtactggatg ttaaagaaat ttttgattat tctggttttg ggcacaattg caaattgtaa
      901 gggaatctct tctacaaaat ctttttctag ttatgatgtg aattccaagg gaaatcgcgt
      961 tcgtaagact ttcactggtc cgatggtggt attcaattcc attcagttcg gagaatccat
     1021 tgatacaaaa gacaaactgc atgaactgct gaaaatcaat gagtgtaaag atctgaagaa
     1081 tatcgaagtt gaattttaca acgactatat tctggttgcc ggataccaga aaatggttat
     1141 cagcgcagac tgttctagag aagaagcagc aaagtaaata ttctattgtc tcgccgtttc
     1201 agcaaacact gaatcgagcg agggattctt ttttagtcag tcgtcctttt tctccagtct
     1261 gaaataaata ttttttcttt tttcgcttga aatcattcag ccgaatcata ccttttctaa
     1321 atgtatgaat gagtctctcc agtctgtttt actgtctaaa tttggagaaa accaagaaaa
     1381 aatcaccgaa tttcagtctc cagccagaat caacattatt ggtgaacatg tggattatct
     1441 gggtggtctg gttctgcccg catccatcaa tttttttact aaaatagcca tccgcccaaa
     1501 ctgggatcat attttcagaa tctactctgt ccagttcaat gagacagtcg aaacaagcca
     1561 gatccttcgg cgaaagacga atacttgggc aaactatatt ctaggggtca tcagcgagtt
     1621 catcaaagaa gggtatatta ttccaggctt tgaccttgtc gtggatggaa atgttcctca
     1681 gggtgcagga ctttcttctt ccgcttctct ggaagtctgt gtcggatatg ctctcagttc
     1741 cgtatttcac cttcctgttt ccagaacaga gattgctgtt tttgcccaaa gagctgaaaa
     1801 taattttacc ggaaccagat gcggaattat ggatcagttc gctgtttcac tgggaaagaa
     1861 ggacaaatgc attcttctga acacagatac attagaatat cattataaat ctgtgcattt
     1921 gggagaatcg gagctttacc tgataaattc caatgtaaaa cattctttgg aaacaagtgc
     1981 atacaatgac agaagaaaag actgtgaaaa tgcactgatc aagataaaga ctaaatttct
     2041 gggtctgaag acactctatc agtttactga aacggacaga atggatctgt tcggcttcac
     2101 tccaaatgaa cataaacggg tgcttcatac cgcaggtgaa agaaaacgga ctgcagaaat
     2161 tatgaaatgt ttcgaagagg gtgatattga gagagcaggg gaaatcctct atcaaactca
     2221 tttttctctc tctgagaatt ttgaagtgtc ctgtcctgaa actgattttc tagtggaaca
     2281 gcttcgcaat ctgaaagtgc agggtgcaag gatggtcgga ggcggattcg gcggatgcat
     2341 tctagttctt gacaaaaaag ggagatatga aaatgtctct gctgaaatac aaaagcttta
     2401 taaggaaaaa tttggaataa atcctgattt ttaccggttt gaaatctgcg acggagtggg
     2461 agaaccctga aatatatgag tgataaaatg gatttaagtt cagctgattt ggcatatgaa
     2521 agttcagaaa taaaaataaa agtaaacaga aaccatcctg atcttccgga agagggagtg
     2581 attgtggata tgtccgggga tctcaacctg tattcaaccc ccaacctgaa aacaatcatt
     2641 accaaactga tggatggcgg atatactaag ttttttgtca atatggaatc tttaaaatac
     2701 attgattctt ccggactggg tgctttcctc agttttcagt ctaaactgct gaaaatgcag
     2761 ggttttatac ggatctgctc gccaagcaaa caggtcagcg ccgttctgga actgactaag
     2821 ctgaaaagta tgttacgagt atccagaaca gtggatgatg caattttcaa cagaacatga
     2881 aagaatacag gacactggca gaattctggc ctttttatct ttccgaacat tctaatattt
     2941 ggaacaggag gctccatttt atcggttcta cctgtgccct tctgagcatt ttttctgttt
     3001 tctattttca gtctgcactg tttcttcttt tggctcctgt ttcaggatac ggatttgcct
     3061 ggttcggtca ttttatcata gaaaaaaaca gaccggctac tttcaagtat ccggtaaaat
     3121 cctttgtttc cg
//
LOCUS       DAOQGJ010000227         3131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18321_length_3131_cov_3.271131, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000227 DAOQGJ010000000
VERSION     DAOQGJ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18321_length_3131_cov_3.271131"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1817
                     /locus_tag="PK683_04805"
     CDS             54..1817
                     /locus_tag="PK683_04805"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HNH07798.1"
                     /translation="MSFFSSFFKLSDMVKFGVFSSDRFTFKGITVQRTKEKNFHVYYE
                     GKKLAQVPAQIDYKPIYDIGQKLNEPFKPPLFGVTCLGPSHGFDPNDNTSGFIIWLNH
                     QGIMVDPPVNSTEWLLDSNVNPKLIDSIILTHCHADHDAGTLQKILEEGKITVYTTKT
                     VMESFLCKYSAFCGVSPEYLSKLFDFVPVKVGKPFFIHSGRFEIFYTLHSIPAIGFRL
                     QFQEKSFAYSSDHNNDPELHKKLFESNVISKARYEELSSFPWNSNVIYHESGIPPLHT
                     PIKYLNSLPEDIQKRLVVYHIAKKDFPEETNLRLAKFGIENTLIFDVDQPQYEEASII
                     LSILKHLDFLSYVPIVKAQEFIGIVKKETYKRGDTILKKGTAGDRFYIIYSGNVSVIN
                     DGLEAKKVYGAYDYFGEVALMKNQPRSADVIAETDVIAYSIHRDEFLNFIADTAFEQT
                     LIRLTKIRDSETWNVLSKSSSLSGLTSTQKTWLESFIVPVEYPASTEILDENSQISHF
                     FIIRNGEVEVRKNGRLLHILSRGDYIGEFNKIYDNTSAGYSFESRGPVSLYSMKRSDI
                     LNFLKHNPGLIMKLRYSFSAK"
     gene            complement(1820..2551)
                     /locus_tag="PK683_04810"
     CDS             complement(1820..2551)
                     /locus_tag="PK683_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07799.1"
                     /translation="MRLFSSPFIGRITILISFFVSASLGADTFKRAFQAYMAKDYKVC
                     RLYSEKYIRESAVNDPASQIFKIYFKTESNLIKLDYVLEKFYKENETLPKEFYALVSI
                     FLEKALVSQNLKLGLKWGERYYLEAAKTKKYFKGLFLYSSLLFLAKEKQRAVSVLTSI
                     DSEKINDEEWKTRLELLRISSLENPKDIIKESKRYLKKHYNEKYSDYLISMMIELYRK
                     SGKREKAEKLQRRLLKDFPRTIFKV"
     gene            complement(2548..2742)
                     /locus_tag="PK683_04815"
     CDS             complement(2548..2742)
                     /locus_tag="PK683_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07800.1"
                     /translation="MAYDEDYNEDEDYDFEDGDDFEEDGKVILACEDCDYRWSPEPDD
                     FSSYESTICPMCGSSNVVEI"
BASE COUNT          964 a          662 c          591 g          914 t
ORIGIN      
        1 accaataaac acgcactagc ttcataaagc ctattcctga cctgcagaag gaaatgagtt
       61 ttttttccag tttcttcaaa ctctccgata tggtgaagtt cggcgttttc agcagtgacc
      121 ggtttacctt caagggaatc actgtgcaga gaacgaagga aaagaacttt cacgtatatt
      181 atgaagggaa aaaactcgcc caggttcctg ctcagatcga ttataagcca atttacgata
      241 ttggacagaa gctcaatgaa cctttcaagc cgcctctttt cggcgtaacc tgtctcggac
      301 cgagtcatgg attcgatcca aacgacaata cctcaggatt cattatctgg cttaaccatc
      361 aggggattat ggtggatcct cctgtcaatt ccaccgaatg gcttctggat tctaatgtaa
      421 atccaaagct catcgattcc atcatactca ctcactgcca tgcagatcat gatgcaggga
      481 ctcttcagaa aattctggaa gaaggcaaaa tcacagtcta tacgacaaag acagtgatgg
      541 aaagcttcct ttgcaaatat tctgcattct gcggggtaag cccggaatac ctttccaaac
      601 tcttcgattt tgttcccgta aaggtaggaa agcccttttt tattcattcc ggaaggtttg
      661 aaatcttcta taccctgcat tcaatccccg caatcggatt cagacttcag tttcaggaaa
      721 aatctttcgc gtattcatct gatcataata acgatcctga actccacaaa aaactctttg
      781 aatccaatgt gatctcaaaa gccagatatg aggaactgag ttccttcccc tggaattcaa
      841 atgtaattta ccatgagtca gggatacctc ctctgcatac accgatcaaa tacctgaatt
      901 cacttccgga agacatacag aaacgtctcg tcgtctatca tatagccaaa aaagactttc
      961 ctgaagagac aaacctccgc ctcgccaagt ttggaatcga aaacactctg atattcgatg
     1021 tagatcagcc ccagtatgag gaagcatcaa ttattctgtc aattctgaag catctggatt
     1081 ttctgagcta tgttcctatt gtgaaggctc aggaattcat cggaatcgtt aaaaaggaaa
     1141 cttataaaag aggcgatacc atactgaaaa aaggcactgc aggggacagg ttttatatca
     1201 tttactcagg caatgtttct gtcatcaacg acggacttga ggctaaaaag gtatatggcg
     1261 catacgatta tttcggtgaa gtggcactga tgaaaaatca gccccgttct gcggatgtca
     1321 ttgcagaaac cgatgtgatt gcatattcga ttcacagaga tgaatttctg aatttcattg
     1381 cagacactgc atttgaacag actctgatca gactgactaa aatcagagac agcgaaacct
     1441 ggaacgtgct ctcaaaaagc tcttctcttt ccgggcttac atccactcag aagacctggc
     1501 tcgaatcttt cattgtccct gtagaatatc ctgcctccac tgaaatactg gatgaaaact
     1561 ctcaaatttc tcattttttc atcatcagga acggagaagt ggaggtaagg aagaacggtc
     1621 ggcttctgca tatactaagc agaggtgatt atataggtga atttaataaa atttatgaca
     1681 atacttcggc tgggtattca tttgaaagca ggggccctgt ttcactttat tcaatgaaaa
     1741 gaagcgatat actgaatttt ctgaaacaca accctggact cattatgaaa ctgcggtaca
     1801 gcttttctgc aaagtaaatt cagactttaa agattgttct cggaaaatcc ttcagaagtc
     1861 ttctctgaag tttttctgcc ttttctcttt tcccgctctt cctgtaaagc tcaatcatca
     1921 ttgaaatcag atagtcggaa tacttttcat tataatgctt tttcagatat cttttagatt
     1981 ctttaataat atctttaggg ttttcgaggg aactgatcct gagaagttcc agacgggttt
     2041 tccattcttc atcgttgatc ttttccgaat caatggaagt aagaaccgag actgccctct
     2101 gtttctcctt ggcgagaaaa agcaggctgg aatacagaaa aagacccttg aaatactttt
     2161 ttgtcttggc tgcttcaaga taatatctct caccccattt caatccaagt ttcagattct
     2221 gggaaacaag cgccttttcc aaaaaaatag aaactaaggc ataaaattcc ttgggaagag
     2281 tttcattttc cttataaaat ttttcgagaa catagtcaag tttgatcaga ttactttctg
     2341 tcttgaagta aattttgaaa atctgactgg ctggatcatt gactgcagac tctcgaatat
     2401 acttctcaga ataaagtctg cagaccttat aatcttttgc catataggcc tgaaaagccc
     2461 ttttaaatgt atctgcaccg agtgatgcag atacaaagaa agatatcagg attgtgattc
     2521 tgccaataaa aggagaggaa aacagtctca aatttctact acattggatg aaccgcacat
     2581 tggacagatc gtcgattcat aggaggaaaa atcgtccggc tcaggactcc atctgtaatc
     2641 acagtcttcg caggctaaaa tgactttacc gtcttcctca aaatcatcgc cgtcttcgaa
     2701 atcatagtct tcatcttcat tgtaatcttc gtcgtatgcc ataattgctt atacagttga
     2761 gaactgcctg cctgtcaatg atttttactg cagataaagg aaaaatgtta tttgaattga
     2821 gcttttatgg aatgtttttc ccacttaggc agattaaaaa gaaaaaagcc gataagcgat
     2881 gcagcagttg agattcccga ttttattcat atgtccgctt gaagtccggc aaaaatgcaa
     2941 agagctccca agctcgagaa aaaccgtatt tgactgaaca gttccgatct gtcccatcaa
     3001 aatggaatgg agagacacat ttattggaac gtctgtattt ttccaaatat ataaaatact
     3061 cgactgcttt gtctgcataa ttatccaagg aaataacgga gaagaaaatg aaaatgtccc
     3121 aagaagcata t
//
LOCUS       DAOQGJ010000228         3128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18351_length_3128_cov_2.599740, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000228 DAOQGJ010000000
VERSION     DAOQGJ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18351_length_3128_cov_2.599740"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1271)
                     /locus_tag="PK683_04820"
     CDS             complement(93..1271)
                     /locus_tag="PK683_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07801.1"
                     /translation="MKNAIRNFALVLLLFFCFPVYAEERKAGESYIHIEWEPVEGGTE
                     YVLQIAADRDFTKILHSQKTKDTLIKLPPNPRYKFARLAGVDSQGITGQFSDIFEIKT
                     RIVKPKPEIVKIEPIPEPPVQKPQRKFLTRQDTVQLVAESRDTVSIFYQSDSPKWKLY
                     TKPISIEKDGRTVIYHFGMDASGKKEPIKSLSVDVDNTPPDVGIEFTNVLQYEMVMYS
                     GPSSRIRISASDSLSGIRETRLLFQSGNDFVEKINAIEEKIPEIFAGKQFFVTALAYD
                     QAGNRTEKTYRFRHDLTPPKIEIFPKPNMKKSWSVGDKFFPKVEDELTGIKYAEYSVN
                     NGGFKILEKEIELNTPGLNIIEIRAWDHAENFSSEKFYFDLTAAETKTNSILNLNDSQ
                     "
     gene            complement(1367..2149)
                     /locus_tag="PK683_04825"
     CDS             complement(1367..2149)
                     /locus_tag="PK683_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNH07802.1"
                     /translation="MKIFILSFIISFSAVFSENFTEYKVQNGDTLSKIAKEKLSDPGK
                     WNELLKYNKIGNPNLIKPGLLLKVPDYLAKDKPAEKPMAKLDMAFGNVKFKKENSTDW
                     QNAEKGQTFFLNDTIKTSEKSGAQIQFMDNPVSTLQLREKSIIKIERQKEVKGFLISM
                     GEALIKTLPGKNNDIKFKISSPSAVAGVRGTEFNVAVDEEESTKLGCFEGLVEVSAQG
                     ETVKVPAGFGTSVKKGEKPVKPYKLLEKVKPKPIIKEDDTEN"
     gene            complement(2354..>3128)
                     /locus_tag="PK683_04830"
     CDS             complement(2354..>3128)
                     /locus_tag="PK683_04830"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNH07803.1"
                     /translation="DVRYDLSNILFVTTANQLDTIPEPLLDRMEIIKLSGYILEEKAE
                     TAKRYLIPKQMKEHGIRDSELKISDSVLSVIADQYARGSGVRSMENQIKKIMRKMALL
                     QAEKNLETAEVTPENIQEFLGPPVFASEELYEKEIRENRFFDEHFIHLHIPAGATPKD
                     GPSAEITLATALYSFAGNIPVKRGLAMTGELTLTGKILPIGGVREKTIAARRVKVYEL
                     IFPKANRRDFEELPEFLKEGIRVHFADFFEEVLKIAFFL"
BASE COUNT          753 a          682 c          642 g         1051 t
ORIGIN      
        1 agtcagggat acggaggcag agcttaaaga cagaatggct gctagtatga agaattgagc
       61 aaatatggaa agcctgatct tcacggtaaa ctctattgag aatcgttcag attcagtatt
      121 gagtttgttt ttgtttctgc agcagtcagg tcaaagtaaa acttttcaga tgagaaattt
      181 tctgcatgat cccaggctct gatttcgata atattcagtc cgggagtgtt cagttcaatt
      241 tctttttcca gtattttgaa acctccgtta ttcacggagt attcggcata tttgattcca
      301 gtcagttcat cttccacttt agggaaaaat ttatcaccga ctgaccagct ctttttcata
      361 ttaggttttg gaaaaatttc tatcttgggg ggtgtaagat catgacgaaa acggtatgtc
      421 ttttctgtcc tgtttcctgc ctgatcatag gcaagtgcag ttacaaaaaa ctgtttcccg
      481 gcaaatattt cgggaatctt ctcttcgatt gcgtttatct tttctacaaa gtcattgccc
      541 gactggaaaa gaagtctcgt ttcccggatt ccgctcaggc tgtcacttgc ggagatcctg
      601 attcttgagg atggacctga atacattacc atttcatact ggagtacatt ggtaaactcg
      661 attcctacgt cgggcggcgt gttgtcaaca tccacagaca gagatttgat cggttctttt
      721 tttcccgagg catccattcc aaaatgatag attaccgtcc tgccgtcttt ctcgattgag
      781 atcggttttg tatacagctt ccatttaggt gaatcggact ggtagaatat ggataccgta
      841 tccctgcttt ctgcaacaag ctgaacggta tcctgtctcg taagaaattt tctctgaggt
      901 ttctgaacgg gaggctcggg gatcggttcg atctttacaa tttcgggctt aggttttact
      961 attcttgtct tgatttcaaa tatgtctgaa aactgacctg taattccctg agaatccaca
     1021 ccggcaagtc ttgcaaattt atacctagga ttcggcggaa gcttgatcag tgtatctttt
     1081 gtcttctgag aatgcagaat tttagtaaaa tctctgtccg ctgctatctg aagcacgtat
     1141 tccgttccgc cttccaccgg ttcccattca atatgaatgt atgactcacc ggccttcctt
     1201 tcttctgcat agacaggaaa gcagaaaaaa agaagcaaga ctagagcaaa attacgaatc
     1261 gcatttttca cgttaaatcc tctgtttaaa ttccctgttt aggattattt tctcaccatg
     1321 gtacaggtgt ccagtctatc ttagtcggaa tacatatctg tatttttcag ttttctgtat
     1381 cgtcttcctt gatgatcggc ttaggtttga ccttttcgag gagcttgtaa ggtttgacgg
     1441 gtttttcgcc ttttttgaca gaggttccaa atcctgcagg taccttgact gtttctccct
     1501 gagcactgac ctctaccaga ccttcaaagc atccaagttt tgtggactct tcttcatcga
     1561 ctgccacatt aaattccgtt cctctgactc ccgcaacggc agatggactg gatattttga
     1621 atttgatgtc attgtttttt cccggaaggg ttttgatgag agcctcaccc atactgatca
     1681 gaaaaccctt cacttccttc tgcctttcaa tcttgatgat ggatttttca cgcagctgaa
     1741 gagtggaaac aggattgtcc ataaactgga tctgtgcccc cgatttttcg gaagtcttaa
     1801 ttgtatcatt cagaaagaat gtctgtcctt tttccgcgtt ctgccagtct gtggaatttt
     1861 cttttttgaa ttttacattt ccgaaggcca tatccagctt tgccatcggc ttttctgccg
     1921 gtttgtcttt ggccagataa tcaggaacct tgagaagaag tcccggtttg atcagattcg
     1981 gattgccgat cttattgtat tttagaagtt cattccattt ccctggatca ctcagctttt
     2041 ccttggctat tttgctcagt gtgtctccgt tctgcacctt gtattccgta aaattttcag
     2101 aaaaaactgc actgaaactg ataataaagg atagtataaa aattttcatg aatgcctcct
     2161 ggagggctgc agaagccgaa attcttccgg ctggttttta atatttcatg ataaaatcgg
     2221 aatgtttttg tttcaagcga aatacttcac cgaatctcaa ttttagaagg ccggttcagt
     2281 tccttcggaa ccgggggagg ggttctgttg tttgtgtctc agtccctgat catttcctgt
     2341 ttaatgataa aatctatagg aaaaatgcga ttttcagaac ctcctcaaaa aaatctgcaa
     2401 agtggaccct gatgccttcc ttcaggaatt ccggaagttc ttcaaaatct cttctgtttg
     2461 ccttgggaaa tatcagttca tagactttta ctctccttgc tgcaattgtc ttttccctta
     2521 cgcctccgat cggcagtatt tttcctgtaa gagtcagttc tccggtcatt gccagacctc
     2581 tttttaccgg aatgtttccg gcaaacgaat acagtgcagt cgcaagagtg atttctgcag
     2641 aaggaccgtc cttgggtgtt gcaccggcag ggatgtgaag atgaatgaag tgttcatcga
     2701 agaatctgtt ttctctgatt tccttctcat aaagttcttc ggaagcaaat acaggcggtc
     2761 cgagaaattc ctgaatattc tcaggcgtga cttctgcggt ttccaagttc ttctctgcct
     2821 gcagaagagc catttttctc ataatctttt tgatctgatt ttccatgctt ctgactccgg
     2881 accccctcgc atattgatcg gcaattacag aaagcaccga atcgctgatt ttcagctcgc
     2941 tgtcgcggat gccgtgttct ttcatctgct ttgggatcag ataacgtttt gctgtttccg
     3001 ctttttcttc cagaatatat cctgataatt taatgatctc cattctgtcc agaagcggtt
     3061 caggaattgt atccagctga ttggccgttg tgacaaacag gatattcgaa agatcataac
     3121 gtacatcc
//
LOCUS       DAOQGJ010000229         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18367_length_3127_cov_3.461589, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000229 DAOQGJ010000000
VERSION     DAOQGJ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18367_length_3127_cov_3.461589"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PK683_04835"
     CDS             <1..1007
                     /locus_tag="PK683_04835"
                     /inference="COORDINATES: protein motif:HMM:NF040119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6493 family protein"
                     /protein_id="HNH07804.1"
                     /translation="STPSVEPFYTDSETLISKLEEYEKAKEEPDMEDLIVACSRVSEK
                     KVSAGLKERIKKLKGNYSSALEYLFGIQDRIKTDKELLPLWTQIARTKDPEGVFKEFE
                     KTSAADYPSVVRPFLPQFSISSGDSKKKEQPRISLINHDGWLHDYAAKTKAVPEEYPK
                     LFYYTSAGAVYNKSVNLDPMHLCLIPHYPDAMLSRYIPKFTSGNDADDCDWVEYGIRF
                     IAENKVRIHHAGWILVGACLLFEKKHIRALGAEYIKFAIEENFLKKEYLSEIIGQMIA
                     EKYGPVVRLMEYIDRRGSSKEEKSLHLEILKNCIQSSKGKELPVNFKKIESAYEELSD
                     "
     gene            1114..2709
                     /locus_tag="PK683_04840"
     CDS             1114..2709
                     /locus_tag="PK683_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HNH07805.1"
                     /translation="MKFFNTAGPNQADIHYTLNPLSRWNLDEILALIEARKYFILHAP
                     RQTGKTSCMLSLMKYLNKEGRYRALYVNVEGAQAAREDVFKAVQGILSRTADCEKNYW
                     NTNTLEMEREAILKKGEMDAITGAFSLLSKKSDKPVVLFIDEIDALIGDTLISVLRQI
                     RSGYADRPESFPQSIVLCGVRDVRDYRIHSSRTQEIITGGSAFNIKAESLLLGNFTKE
                     EIASLYAEHTKETGQTFEDGILDLAWDYTGGQPWLVNALAYEACFRTAESKDRARPIT
                     VEMFKDAKESLILRRDTHLDQLTDKLKEDRVRRIIEPMLKGDDSTYQFNDDDAQYVID
                     LGLITRKPNKELNISNEIYREILPRELTSGWQYGLNYQNTWYVDKVTNKIDFYKLLKA
                     FQEFFQENSEIWIDRFAYREAGPQLLLQAFLQRIVNGGGVINREYGLGTRRTDIYVKW
                     YPDEKNHSVSQKAVIECKLLHKTLEKTLQEGLEQVSAYSDKCSAEESHLVIFDRTKDR
                     PWEEKLFIKEMEFEGRKITVWGM"
     gene            2822..>3127
                     /locus_tag="PK683_04845"
     CDS             2822..>3127
                     /locus_tag="PK683_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07806.1"
                     /translation="MTLSSYEYNYTDSSAFIEENSEKKFLLSHCTEIEEDQKIPCFFY
                     GKFIDSFIISRCLSVLARTVKSHFSIRPEQRAAMRDPIVSVGSGEIRFEGFSSCAGVY
                     "
BASE COUNT         1017 a          611 c          697 g          802 t
ORIGIN      
        1 tatccactcc ctccgttgaa cctttttaca ctgattcgga aactctgatt tcaaagctgg
       61 aagaatatga gaaggcaaaa gaagaaccgg atatggagga tctgatcgtt gcctgcagca
      121 gagtctctga aaaaaaagtt tcagccggtc tgaaagagag aatcaaaaaa ctcaaaggaa
      181 attattcttc tgctctggag tatctttttg gaattcagga caggataaaa acggacaagg
      241 aactccttcc tctctggaca cagatcgcaa gaacaaaaga tcctgaaggc gtattcaagg
      301 aatttgaaaa aacttccgca gcagactatc cctctgttgt ccgtccattt cttcctcagt
      361 tcagcatcag ctcaggagat tccaagaaaa aagaacagcc aagaatatca ttaataaacc
      421 atgatggctg gcttcatgat tatgcagcaa agactaaggc cgttccagaa gaatacccta
      481 aactgtttta ttatacatct gcaggtgcag tctacaataa atcagtcaac ctggatccta
      541 tgcacctgtg tctgattcct cattatccgg atgcaatgct ttcccgttat attccaaagt
      601 ttacctcggg gaacgatgca gacgactgtg actgggtgga atatgggata cgttttattg
      661 cagaaaataa agtccgcatt catcatgcag gctggattct tgtcggagcc tgtcttctgt
      721 ttgagaagaa gcatatcagg gcccttggcg cagaatacat caagtttgcg atagaagaaa
      781 attttctgaa gaaggagtat ctttctgaga tcatcgggca gatgatcgca gagaaatatg
      841 gacctgttgt ccgtctgatg gagtacattg acagaagggg aagctcaaaa gaagaaaaaa
      901 gtctgcatct cgaaattctg aaaaactgca ttcagtcttc taaaggaaag gaacttccgg
      961 tgaatttcaa aaagatcgaa tctgcatatg aagagctttc tgattgaatc ttatcattat
     1021 aaaacctatt ttcttccctg tcattctttt ggacagtgca gcatttaatt acagcttgac
     1081 agtttcatct gctgaaagca ggatagggaa aatatgaaat tcttcaatac agccggacca
     1141 aatcaagcag atattcatta cacgctgaat cctttgtcca ggtggaattt ggatgaaatt
     1201 cttgctctca tcgaagcaag aaaatacttc atccttcatg ctcccagaca gacagggaaa
     1261 accagctgta tgctatctct gatgaagtat ctgaacaagg aaggcagata cagagcactg
     1321 tatgtaaatg ttgaaggagc acaggcagcc agagaagatg tttttaaagc cgtgcaggga
     1381 attttgagca gaactgcaga ctgcgaaaaa aactattgga atacaaacac attagaaatg
     1441 gaacgggaag ctattttgaa gaaaggggag atggatgcca ttacaggagc attttcccta
     1501 ttatcaaaaa aatcagacaa accagttgtt ctgtttatag atgagataga tgctctgatc
     1561 ggagatacat taatttctgt tctcagacag attcgttccg ggtatgcaga cagaccggaa
     1621 tcctttccgc agtccattgt tctctgcggt gtgcgggatg tccgcgacta tcgaattcat
     1681 tcttccagga cacaggaaat cattacaggc gggtcagcgt tcaatatcaa agcagaatcc
     1741 cttttactag gaaattttac aaaagaagaa attgcatcac tctacgcaga gcacacaaaa
     1801 gaaacgggac agacatttga ggacggaatt ctggatctgg cctgggacta taccggcgga
     1861 cagccgtggc tcgtcaatgc tctggcttat gaagcctgct tcagaaccgc tgaatccaaa
     1921 gacagggcca gacccattac tgtggaaatg ttcaaagatg ccaaagaaag tctgatattg
     1981 agaagggaca ctcatttaga tcagctcaca gacaaactga aagaagaccg agttcggaga
     2041 attatcgaac caatgttaaa aggcgatgat tctacatatc agtttaacga cgatgatgca
     2101 cagtatgtga ttgatctggg actgattacc agaaagccga acaaggaact gaatatatcc
     2161 aatgaaattt accgggagat actgccgagg gaactgacct caggatggca gtatggattg
     2221 aactatcaga acacatggta tgttgataaa gttacaaata aaatagattt ttataagctg
     2281 ctcaaagcgt ttcaggaatt ttttcaggag aattcagaga tttggattga ccggtttgca
     2341 tatcgggaag caggaccgca gcttttactg caggcatttc tgcagcgcat cgtaaacgga
     2401 ggcggtgtca tcaaccgtga atacggtctc ggcacaagaa gaacagacat ttatgtaaaa
     2461 tggtatcccg acgagaaaaa tcattctgta tcacaaaaag ctgtcattga atgcaagctt
     2521 cttcataaga ccctagagaa aactttgcag gaaggcctgg aacaggtctc ggcatactcc
     2581 gacaagtgca gtgcggaaga atcgcatctt gtcatttttg acagaaccaa agacagaccc
     2641 tgggaagaaa aacttttcat aaaggaaatg gaatttgaag gcaggaaaat tactgtctgg
     2701 ggaatgtagg aaaaacaaag tctgctcagt tctgctcctt caagatgaca gaggaaaaaa
     2761 gctgttccta agtgtgactt gacaaaggtt catcaaaaag aacagaatac aagggagaaa
     2821 tatgacactt tcaagctacg aatacaatta cacagattca tccgctttta ttgaagaaaa
     2881 ttcagagaag aaatttctcc tttctcactg cactgaaatc gaagaggatc agaagattcc
     2941 ctgttttttt tatggaaaat ttatagattc tttcattatc tccagatgtc tgagtgttct
     3001 ggcaagaaca gtaaaatcgc atttttccat ccgacctgag cagagagctg caatgagaga
     3061 tccgattgta tctgtaggca gcggtgaaat acggttcgag ggattttcct cctgtgccgg
     3121 tgtatac
//
LOCUS       DAOQGJ010000230         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18396_length_3124_cov_3.112740, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000230 DAOQGJ010000000
VERSION     DAOQGJ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18396_length_3124_cov_3.112740"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            607..1023
                     /locus_tag="PK683_04850"
     CDS             607..1023
                     /locus_tag="PK683_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HNH07807.1"
                     /translation="MSKYVHYALYVFLSLSVTNCEKFGLLGQGGVPGSTVKSEIVAAA
                     TASSAISNTAVCASLGSSYASFCNSYVGLSVIMDTIIAPITAKVSDSKHYLRSSVDEC
                     KSKIRSIGFIMNTGLSGITCNLKESPKVIQIDETGF"
     gene            1263..1520
                     /locus_tag="PK683_04855"
     CDS             1263..1520
                     /locus_tag="PK683_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07808.1"
                     /translation="MSTPSGKIRHYQTTTNFGLHILGLYPVWGNASLNGTVNQFTQNA
                     LKERANSVHIIQKDMTRLYLLLPPLTLIFMPVVTEVFGEVY"
     gene            1525..2163
                     /locus_tag="PK683_04860"
     CDS             1525..2163
                     /locus_tag="PK683_04860"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07809.1"
                     /translation="MPIFQQIVTKASEDRLEKLIQERVSASPELKKEIDARVWDLFGE
                     TWSIMFTDLSGFSRNVEKFGITHFLQIIYESHRLLVPEIDNYDGILLKKEGDSMMIIF
                     RKPDKAVDCAVQMQKTAAQYNSGRPDEEKILLCIGLGHGLILKIGDDDVFGAEVNAAS
                     KLGEDTAKSGEILVTGSMMEILSERKDLSFEMLENIPPGAQSSYLLKSAEHP"
     gene            2310..2909
                     /locus_tag="PK683_04865"
     CDS             2310..2909
                     /locus_tag="PK683_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH07810.1"
                     /translation="MPKNQILSKISSKPTLREREKFLLQKIRSGDKAAFMELVSPFQE
                     RLFRKAKSMVGDDDDAEDIVQDALISGYKSIAKFRADSGVYTWLYRIVVNKSKDLILK
                     KKKSNEKPLNDNDQTFFDERLSYEKKLELSDESAYLIKKINMLEPMYRQVIDLRYYEE
                     LSYSEIADLLKCNVGTVKSRIFKAKELLKHLILNDEKEK"
     gene            2913..>3124
                     /locus_tag="PK683_04870"
     CDS             2913..>3124
                     /locus_tag="PK683_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07811.1"
                     /translation="MNDRDTKWLGFFSWDTETESEHDQRILENRLVQSISEYRKKELE
                     NIQLSENFNERLKAQLSMTESESYNET"
BASE COUNT         1004 a          626 c          628 g          866 t
ORIGIN      
        1 ccgatgacaa actgagagag aagaccccat ttgattgtct cccagggaat cagtttttta
       61 ttttcagaag tcagccatgc caatatgcag agcacataca cgcctataag agagatcaca
      121 ttcagtttta aatccatatt gcccttgccc gcagttccag acccaatcca gtcgtacagc
      181 ctgaagaaag atactaaaat aagtatcgac aactgtgtaa ggaaaatcaa ccatcacgaa
      241 tatatttgga tcagccgagt ccattgaaaa ggagcaggca ttctgaatgc cgaaaaaaag
      301 acgcttcaaa acaaatcact cagcacagca gaaacacagt tgcctggttt aaccttcctc
      361 ccaatataaa tcatccaact gcatattaaa atgatgcgaa atattgatct ttccctgcaa
      421 aaaggtcttt aactttaggt ttatgatttt tttgactctt tttgcaatat ttaaccattt
      481 ttttcttgac ttttcttgcc atagaaaata aatgtacaga aaatttaaca ataaacttca
      541 ttttagggac tatgggacac tttaaaccac tgtcacccta gatgagattt ttaaaaagga
      601 tcaaaaatgt cgaaatacgt tcattacgct ttatacgtgt ttttatcact ttcagtcaca
      661 aattgtgaaa aatttggctt attgggtcag gggggtgtac caggaagcac tgtcaaatct
      721 gaaatagtcg ctgccgccac agcttcttcc gctatcagca acactgctgt atgtgcatct
      781 ttaggctcaa gttatgcttc tttctgcaat tcctatgtag gcctttctgt cataatggac
      841 actattattg cgccaatcac tgcgaaagtt tccgactcaa agcattatct gagaagttca
      901 gtagatgagt gcaagtctaa gattaggtcc attggattca ttatgaatac aggtctttcc
      961 ggaattacat gtaatttgaa agaatcccca aaagtcattc agattgacga aacaggtttt
     1021 taacgctgac agcagtaaaa cctatctcgg gttttactgc ctccctttcc atttcagttc
     1081 cttcgtcggc tcctgtaaaa attttccgac gcacaaaatt tgaactttct tcttattcct
     1141 attttcactt gacatctgag ggattttttt tcatttggga gtctatgaaa ctgcttcaac
     1201 ttattctgct tataatgctt attggatgct cctctacaag tcacataaaa gactcaagca
     1261 atatgagcac tccctctggc aagatcagac attatcagac aacaacgaat ttcggtctgc
     1321 acattttggg tctctaccct gtatggggca atgccagcct gaacggcaca gtcaatcagt
     1381 tcacccaaaa tgcactgaaa gaaagagcaa acagtgtaca tatcatacag aaagatatga
     1441 caaggcttta ccttctgctt cctcccctga cacttatctt tatgcctgtc gtcactgaag
     1501 ttttcggcga agtatactga atcgatgcct atctttcagc agattgtaac caaagcctct
     1561 gaggacagac tcgaaaaact gattcaggaa agagtcagtg cttcacctga actgaaaaag
     1621 gaaatcgatg ccagagtctg ggatcttttc ggtgagactt ggagcataat gtttacagat
     1681 ctttccggtt tctccaggaa cgtggaaaaa ttcggcatca ctcatttcct gcagatcatc
     1741 tatgaatcgc acaggctcct tgtgccggag atagacaact atgatggaat ccttctgaaa
     1801 aaagaagggg acagtatgat gattatcttc agaaaacccg acaaggctgt ggattgtgct
     1861 gttcagatgc agaaaacagc ggcacagtac aattcgggca gacctgatga ggaaaaaatc
     1921 cttctttgca tcggccttgg acacgggctg attctgaaaa tcggggatga tgatgttttc
     1981 ggagcagagg taaacgctgc aagtaaactt ggtgaggaca ctgcaaaatc aggagaaatt
     2041 cttgtgaccg ggtcaatgat ggaaatcctt tcagaaagga aagacctctc gtttgaaatg
     2101 ctggaaaata ttcctccggg agctcagtct tcctatctgc tgaaatcagc ggagcaccct
     2161 taaaaggcca agagagaaac agtatacatt ttcgtgatat gagaatatcc gtttttctgt
     2221 tatcaggaca ttctgtattc tcaatggggc ttgacaaagt ctgattcatt gacacactgg
     2281 atacagcagg tgtgaatgtg ctcaacctaa taccaaagaa tcaaattctg tccaagattt
     2341 catccaagcc tactttgcgg gaaagggaaa aatttcttct ccagaaaatc cgttcaggcg
     2401 acaaggctgc atttatggaa ctggtttctc cttttcagga aaggcttttt cgcaaggcaa
     2461 aatcaatggt aggagacgat gatgatgcag aagacattgt acaggatgca ctaatttcag
     2521 gatacaagtc tattgcaaaa ttcagggcag attcaggagt atacacatgg ctgtacagaa
     2581 ttgtggtgaa caagtccaag gacctgattc tgaaaaagaa gaaatcaaat gaaaaacctc
     2641 tgaatgataa tgatcagact ttttttgatg aaagactcag ctacgaaaaa aaattagaac
     2701 tatctgatga atcagcctat ctaataaaga agataaatat gctggagccg atgtacaggc
     2761 aggtgatcga tctgcggtac tacgaagagc tttcctactc tgagattgca gatcttctga
     2821 aatgcaatgt aggcacagtg aaaagcagaa tcttcaaggc aaaagaactg ctgaaacatc
     2881 tgattttgaa tgacgaaaag gagaaataaa ctttgaatga cagagataca aaatggttag
     2941 gttttttttc ctgggataca gaaactgaat cagaacatga tcagagaatt ctggaaaaca
     3001 ggctggtgca gagtatttca gaatacagaa aaaaagaact ggagaacatt caactttcag
     3061 agaatttcaa tgaaagactt aaagcacagc tttctatgac agagtctgaa tcctataatg
     3121 aaac
//
LOCUS       DAOQGJ010000231         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18407_length_3123_cov_3.453390, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000231 DAOQGJ010000000
VERSION     DAOQGJ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18407_length_3123_cov_3.453390"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1331)
                     /locus_tag="PK683_04875"
     CDS             complement(222..1331)
                     /locus_tag="PK683_04875"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNH07812.1"
                     /translation="MHEKTEQFIQSAMQEIREKNRFRSVSVPKGTDFSSNDYLKLSES
                     PELKAAFAEYLEKDGLGSGASRLLRGHRDVFTEAEEIFSRYVGTEDSLFLANGFLANL
                     GLADLLGKSSPVFLCDRLNHASILDGIRLSGAEVRFYRHNDSNQLRDLLLKYRDRETT
                     VISETIFSMEGDFYRKDEIAALKKEFNFTLVLDEAHALGVYGENGRGLSCQSDADFRV
                     FTCGKALGLEGAFISCRRDLKEYLINAMRTFIFSTAPIPAVAFCAGRSVRLAEKMEVQ
                     RNLIHSNADLLRKGLRELGFRTADSRSQIVPVLLGDEKTSLQYSSVLKEKGFDIRAIR
                     PPTVKESRLRISLNSGISASDIENLLEVFSDLQRK"
     gene            complement(1331..2761)
                     /locus_tag="PK683_04880"
     CDS             complement(1331..2761)
                     /locus_tag="PK683_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNH07813.1"
                     /translation="MNLFRRIFLSAAVLFSLNHCIPYWEKLHSVSLQDIQKRGVLKAY
                     MAYSQDNYFIYKDEEMGFEYELLNLFAKEIGVRLEVTPVHDLDTLPYMLNSQDGDIVA
                     SNIAVTKERQKELIFTEHLVSTRQVLVQKKKEKAAPGKYVSNAVELIGKKVHLREKSS
                     FYFRMKDLENEIGGDIDLATVPGTVSNEELIEQVAQGTDVDYTIADEPIALINQTYYP
                     DLDVSMPVSFPQKIAWAVRRNSPEFLKRVNQWIYKIKGDGTLQAIRKKYYKDQKTVFF
                     DDSKEATKEEKIFEKEKKQGRKRISPYDETIRAKSSQIGWDWRLVAAVIYQESKFKPR
                     ARSWVGAAGLMQIMPGTAAAMGLAAGDIFHPEKNIQAGVKYLGYLDNYWKKVLPNAEE
                     RKKFVLASYNAGQGHVVDARSLAAYLGKNPSIWDGNAASAMLLLSNPAYFNLSVVRHG
                     YCRGKEPYEYVNIIYRQYSAYKKITE"
     gene            complement(2781..>3123)
                     /locus_tag="PK683_04885"
     CDS             complement(2781..>3123)
                     /locus_tag="PK683_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07814.1"
                     /translation="QNISPLSAVHFSGIDFSAAGKLFLDLKTDLSADSAQIIYWSPGN
                     EFRSASGSVISGKRISAVLNVPASERDKGMLIIRSPEKNYFGCFVSSGISSNALDFIS
                     AKLQDRCDTLN"
BASE COUNT          816 a          757 c          616 g          934 t
ORIGIN      
        1 gaaaaacaag aatcttgtct tccttcagcc tgctttccag tttggattta ggcgaagact
       61 tgctccagat attgtacaga ccagtcagaa gcagcacaat tacagtaata agaatacttc
      121 tttttaaatt aatatatgta aacaaaaaga accttttcct cccgaacagt ttttcgccgg
      181 atgcaatact aaggaaagct ccatttaatt tcagtcaacg attatttcct ctgcagatct
      241 gaaaatactt caagaaggtt ctctatatcc gatgcagaaa ttcccgaatt cagactgatt
      301 cgaaggcggc tttccttgac tgtaggagga cggatcgcac gaatatcgaa tcctttttct
      361 ttcagaacag aggaatactg aagagaggtt ttttcatctc ccagaagaac cggaacaatc
      421 tgcgacctgg agtcggctgt tctgaaccca agttccctca gtccctttct cagaagatct
      481 gcattcgaat gaatcagatt tctctgcact tccatcttct ctgccagacg aactgatctt
      541 cccgcacaga atgcgactgc aggaatcggt gcagtggaaa agataaacgt tctcattgca
      601 ttgatcaggt attccttcag atcccttctg caggaaatga atgctccttc cagtcctaaa
      661 gccttcccgc aggtaaatac tctgaaatcg gcatcactct gacagctcaa acctcttccg
      721 ttctcaccgt agacacctaa agcatgtgcc tcatcgagga ccagtgtaaa attgaattct
      781 ttcttcagtg ctgcgatttc atccttcctg taaaaatctc cttccatact gaatattgtt
      841 tccgaaatca ctgtagtctc tctgtctcgg tatttcagaa gaaggtctct gagctgattg
      901 gaatcattgt gcctgtaaaa acgcacctct gcgcctgaaa gacggattcc gtcgagaatg
      961 gaagcatgat tcagtctgtc acagagaaag accggggagg attttcccag aagatctgct
     1021 aatcctaaat ttgccaaaaa tccgtttgca agaaaaaggg aatcttccgt tccgacatac
     1081 cttgaaaaga tctcttcagc ctcagtgaat acatccctgt gtccacgaag aagtctggat
     1141 gctccggatc ccaggccgtc tttctccaga tactcggcaa atgccgcctt aagttcaggg
     1201 ctctctgaaa gcttcagata atcgtttgat gaaaaatcag tccccttcgg tactgaaaca
     1261 gacctgaatc tgtttttttc gcgaatttcc tgcattgccg actgaatgaa ctgttctgtt
     1321 ttttcgtgca ttattctgta atttttttgt atgctgaata ctgtctgtaa atgatgttca
     1381 cgtattcgta aggttccttc cctctgcagt acccgtgccg cacaacagaa agattgaaat
     1441 aggccgggtt ggaaagaaga agcattgcgc ttgccgcgtt tccgtcccag atactcgggt
     1501 tctttcccag atatgctgca agcgacctgg catccaccac atggccctgt cccgcattgt
     1561 atgatgcaag aacgaatttt tttctttcct ccgcattcgg aaggaccttc ttccagtaat
     1621 tgtcaagata gcccagatat tttactccag cctgtatgtt tttttcagga tgaaatatat
     1681 cacctgctgc aagtcccatc gcagcagccg ttcccggcat aatctgcatc agtccggccg
     1741 cacctaccca ggatctggcc ctgggcttga atttggattc ctgatagatg acggctgcaa
     1801 ccagcctcca gtcccagcct atctgagagg actttgcacg gattgtctca tcataagggg
     1861 aaatacgttt tcttccctgc ttcttttcct tttcaaaaat cttttcttcc tttgtagctt
     1921 ccttggaatc atcaaagaaa actgtcttct gatccttgta gtatttcttg cggattgcct
     1981 gaagtgttcc gtctcccttg attttataaa tccactgatt gacccttttc agaaattcgg
     2041 gagagtttct tctgactgcc catgcaatct tctgaggaaa gctgacaggc atactgacat
     2101 cgagatccgg ataatatgtc tggttgatga gtgcgatggg ttcgtctgca attgtataat
     2161 ccacatcagt tccctgtgca acctgttcaa tcagttcttc attggaaaca gtgcccggca
     2221 cagtcgcaag atcaatgtcc cctccgattt cattttccag atccttcatc ctgaaataaa
     2281 atgaggactt ttctctgaga tgcacttttt ttccgatgag ttcaactgcg ttcgatacat
     2341 attttccagg agcagccttt tcctttttct tctgtacaag cacctgtctt gtagagacca
     2401 gatgttctgt gaatatcagt tccttctgtc tttcctttgt gactgctatg tttgacgcga
     2461 caatgtctcc gtcctgactg ttcagcatat aaggaagagt gtccagatca tgcacaggag
     2521 tcacttccag tctgactccg atttcctttg caaaaagatt cagaagttca tattcaaatc
     2581 ccatctcctc atccttgtag atgaagtaat tgtcctgact gtaagccata taggctttca
     2641 gaacgcctct tttctgaata tcctgaagac tgacactgtg gagtttttcc cagtacggga
     2701 tacagtggtt cagagaaaag agaacggccg ctgaaagaaa aattcttctg aaaagattca
     2761 ctttttaaat atcggcatct tcagttcaga gtatcacacc tgtcctgcag ttttgctgaa
     2821 ataaaatcca gtgcatttga ggaaattcct gatgaaacga agcatccaaa ataatttttt
     2881 tcagggcttc tgatgatgag cattcccttg tccctttccg atgcgggaac attcagaact
     2941 gcgcttatcc gtttccctga aatgactgaa cccgatgcag aacggaattc gtttccagga
     3001 ctccagtaaa tgatctgtgc cgagtctgca ctcagatccg tcttcagatc cagaaaaagt
     3061 ttacctgcag ctgaaaaatc aatcccggaa aaatgaacag cagaaagtgg acttatattc
     3121 tgc
//
LOCUS       DAOQGJ010000232         3118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18453_length_3118_cov_2.863859, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000232 DAOQGJ010000000
VERSION     DAOQGJ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18453_length_3118_cov_2.863859"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1094
                     /locus_tag="PK683_04890"
     CDS             87..1094
                     /locus_tag="PK683_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNH07815.1"
                     /translation="MAKIYSLVNLKGGVGKTTLAVNIAAGLANFPRPEGIPFNVLLID
                     ADPQSNASIYMLGEEYWRKEIYSKLVNSPNLYRLLDTMVNGGGIFSYDDFIIKERTDA
                     PNPIFNRMQYRRSDGKEEFREAKMTWPNLHLLAAIPKLNEISEDLVSIHTLNRRFEKY
                     GQLKKITSAFENEYDYIIIDCPPNFSPLTKNAIAASHNLIIPYIPDYMSTVGLNELLA
                     NISENQERQDLSVQAFIPTLYNDMSKDYRKFIEQVNEDIMEKIRKQNPSAANILNRKN
                     TGLFYTGLKRSVSVSSAIESHRPIIDLSISSDSRKEIDLIVDFIIDPQRMRRGSRNEQ
                     Q"
     gene            1081..1659
                     /locus_tag="PK683_04895"
     CDS             1081..1659
                     /locus_tag="PK683_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07816.1"
                     /translation="MNSNDVIKLLQDFQKLRMDLLKPEKILSAGLQLKKIYSEVESEV
                     RLLELEKRESELKSYHESIYTKYYKKKMSDQKIEKPDRRNYSEYERYIGLLETFYQRQ
                     EKDLQKYLQILKKIRSVPDAESIQILSDLLAEDLENIELLTELKLKQNVLILKQLTGK
                     SDKESARKTKKIEDFLIKSLSSFKQMKETGIS"
     gene            complement(1661..>3118)
                     /locus_tag="PK683_04900"
     CDS             complement(1661..>3118)
                     /locus_tag="PK683_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH07817.1"
                     /translation="TLSHSFSTPDFGNKFWSENMKKLLILFFLISCTGTEMIRFRDTP
                     SSGYVIHSVRIFSGRADRPVQQNMDVHIQNGVIQKISKTPLQIADAKEIDGKGRVLMP
                     GLVDFHTHITAGMFIPWKASLLPTQDFNLEAAVFSGITTVVDMSGKETSEMNSLKKNL
                     EEGKQNGPHLYHSGIGFTAKGGHPVRYLDVVREKIFFLLHPFIPKIAIEMEENTQDFS
                     ALEKHLAGKPDFTKVYVDKIPETANLISDDLLKKIVEKSHSAGVPVVLHIGDNANLAS
                     LINSGADGAVHAVYKEKINQNLARKLASSGIYVVPTIIVWDNFDKFKNRQSYSHYTSL
                     ETATTYPDRLEALKNPVPEKNKKQDWEEADKKVSENIPTLFENVRIMKKAGVKMVAGS
                     DSPNYGISIGGSLHVELQNLVKAGFTPAEAIQAATYTPASILDKNLSFGTVEEGKRAD
                     LLLVEGDPLKNIKDTENIAEVFVSGRRIERFGRKE"
BASE COUNT          936 a          662 c          622 g          898 t
ORIGIN      
        1 tttttgttct gacccagaaa cgaaatttcg aaagacgcga acgctgattt tggaaaaaac
       61 ccttctgaaa atggaacagg gttaaaatgg cgaaaatata ttctctcgta aacttaaagg
      121 gcggggtagg caaaactacc ctggctgtca atattgcagc cggtcttgcc aattttccca
      181 gacccgaagg cattcctttc aacgtactgc tgattgacgc cgacccacag tcaaacgctt
      241 ccatctatat gctcggtgag gagtattgga gaaaggaaat ctattccaaa ctggtaaatt
      301 ctcccaatct gtacagactt ttggatacga tggtcaacgg aggggggata ttttcttatg
      361 atgattttat cattaaggaa agaacagatg cacctaatcc gattttcaac cgaatgcaat
      421 acagacgttc agacggaaag gaagaattca gagaagcaaa gatgacctgg ccaaatctgc
      481 atcttcttgc agccattccg aaactcaatg aaatttcaga agaccttgtg tccattcata
      541 cactgaacag gcgtttcgaa aaatacggtc agctaaaaaa aatcacttcg gctttcgaaa
      601 acgaatacga ctacattatc attgactgtc ctcccaattt ttctcctctc accaaaaatg
      661 ccattgcagc atctcataat ctgatcatac catatatacc ggattatatg tctactgtag
      721 gattaaatga gctgcttgca aatatttctg aaaaccagga aagacaggat ctgtcggtac
      781 aggcattcat tcctactctc tacaatgata tgtcaaaaga ctacagaaaa tttatagaac
      841 aggtgaatga ggatattatg gagaaaatca gaaaacagaa tccttctgcg gcgaatattc
      901 tcaaccgaaa aaacacaggt cttttttata ctggactgaa gcgttctgtc tctgtatcat
      961 ccgcaattga atcacacaga ccgatcattg atctttcgat ttcatccgat tcaaggaagg
     1021 aaatagacct gattgtcgac tttatcattg atccgcagag aatgagaagg ggaagcagaa
     1081 atgaacagca atgatgtaat caaacttctt caggattttc agaaattgag aatggatctt
     1141 cttaagccgg agaagattct gtctgcaggc cttcagctga agaaaatcta ctcagaagtc
     1201 gagtcagaag tcagacttct tgaactggaa aaaagagaat cggaactgaa gagttatcat
     1261 gaatctatat atacaaagta ttataaaaag aaaatgtccg atcagaagat agaaaaaccg
     1321 gacaggagaa attattccga atatgaaaga tatatcggtc ttctggaaac gttttatcag
     1381 cgtcaggaga aagatctgca gaagtatctt cagattctga agaagatcag atctgttccg
     1441 gatgcagagt ccatacagat tctgtctgac ctgcttgcag aagatctcga aaacattgaa
     1501 cttctgacag agctgaagct caagcagaac gtactgatat taaagcagct gacaggaaaa
     1561 tcagacaagg agtctgccag aaaaacaaag aaaattgaag attttttaat taagtccctt
     1621 tcatcgttca agcagatgaa agagaccgga atcagctgag ttattccttt cttccaaacc
     1681 tttctattct tcttccgctg acaaatacct ctgcaatatt ctctgtatct ttgatgtttt
     1741 tcagaggatc cccttccaca agcagaagat ctgccctttt gccttcctcc actgttccaa
     1801 aacttagatt tttgtccaga atggatgcag gagtataagt cgctgcctgt atggcttctg
     1861 cgggagtaaa gcctgcctta accagattct gaagctcaac atgcagggag cctccgatgg
     1921 aaatcccgta attaggagaa tcacttcctg caaccatttt taccccggct tttttcatga
     1981 tccttacatt ctcaaaaaga gtcggaatgt tttctgaaac ttttttatcc gcctcctccc
     2041 aatcctgttt cttattcttt tcaggtactg gattttttaa cgcttccagt ctgtccggat
     2101 atgtcgttgc agtttcaagt gatgtataat gggagtagct ctgcctgttc ttgaatttgt
     2161 caaaattatc ccatacaatt atagtaggca ccacatatat tccggaagaa gcgagtttcc
     2221 ttgcaagatt ctggtttatc ttttccttat agaccgcgtg aacagctccg tccgctcctg
     2281 aattgatcag tgatgccagg tttgcattgt cccctatatg aagcacaact gggacgcctg
     2341 cagaatgaga tttttctaca atctttttca gaagatcatc tgaaatcagg tttgctgttt
     2401 caggaatctt atcaacatat acttttgtaa aatcaggttt gccagcgagg tgtttttcca
     2461 gagcggaaaa gtcctgtgta ttctcttcca tctcaattgc gatttttgga atgaacggat
     2521 gaaggagaaa aaaaatcttt tctcggacaa catccagata ccggactggg tgtcctccct
     2581 tggctgtaaa cccaattccg gaatgataga gatgggggcc attctgcttt ccctcttcca
     2641 gattcttttt caaggaattc atttcagatg tttcctttcc gctcatatcc acgactgtcg
     2701 ttatgccgct gaagactgct gcttccaaat tgaagtcctg agtggggaga agtgatgctt
     2761 tccagggaat gaacatcccg gctgtgatat gtgtgtgaaa atccacaagt cccggcatca
     2821 gaacccttcc ttttccgtca atctccttcg catctgcgat ctgcagcgga gttttggaaa
     2881 ttttctgaat gactccgttc tgaatatgta catccatatt ctgctgaaca ggtctgtctg
     2941 ctctgcctga aaaaattctg acactgtgaa tgacatatcc tgaagacggc gtatctctga
     3001 aacgaatcat ctcagtgccg gtgcagctga tcagaaaaaa aaggattaaa agttttttca
     3061 tattctcact ccaaaattta tttccaaaat ccggtgtact gaaagaatgc gatagggt
//
LOCUS       DAOQGJ010000233         3104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18585_length_3104_cov_3.627091, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000233 DAOQGJ010000000
VERSION     DAOQGJ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18585_length_3104_cov_3.627091"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1530)
                     /locus_tag="PK683_04905"
     CDS             complement(271..1530)
                     /locus_tag="PK683_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH07818.1"
                     /translation="MIRSAANFIVSLTFCFSIFADELNGKLDIWAVMEIARKQNPKLL
                     AERMSVKKAVGDVVTSRLYPNPVFNNQLVVSPHSDPGSSRFSPQNTQGYFQISQPIPV
                     AGQRTYAIERALKSLEAEQRNVEEFERNVLYITASKWMDVWFTLERLSVIRRTADFSK
                     ELLKVNELRMKNQVITQAEYFRTLNLVETYEATLISAEQEYKNERRNLRALLGYEQGD
                     FTIDSKELPALPDQEINSDDLKKKSFDLRPDYQYRISTESVAKANIRLQNARAYPMPE
                     FGFVANPQNGRDFYGTYINIPIPVNNRNQGEILQAKADLEKAEYLTDGLKKTILAEVD
                     NSIESFSTAKINYKRIQKIFETSEKILQTVRYGYVKGGTQIIDYLDAQRNWFDAQNRL
                     LEAKFILQKTYLDVLYSSGRILNWKKQ"
     gene            complement(1499..>3104)
                     /locus_tag="PK683_04910"
     CDS             complement(1499..>3104)
                     /locus_tag="PK683_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH07819.1"
                     /translation="LETEIILSGVSWYNKAMDILSGKQTSVLMSVFICTMIISSFSFL
                     KGRNFFPALNEGSLWIRVFLSPGISLEAAKIYPEKIREHISLIPDVEQIVTQLGRNDN
                     GTDPYGFNRMEILVNLKRSVFTDFSKISGQISEILKNNFPGMAFSMTQPIRDTLMENA
                     SGTSADIAIDIYAKTGIDRKFAEDTLEAVRQYGELEETYIEQEGRQSVVKIRINREES
                     ARLGINVSDINDVLKTAIAGVPISYLYEDEKKFNIVMKYTKEIRSTTEALGKILIPGK
                     SGQRIPLQRIADIRLEEGESVIYRENGERLYTVRATLKKRNISYITEKLTAKIREKHG
                     DKSRYFNAGGQYGNLKRLESKLSETIPLLFMVLFIVLLVIYQMNLRYVILIFAGSIFS
                     VAGSGIILLLFGSNWDTSSYAGLILSSGLGMFLPILVMNTLMEASEHNMDNYLIIKLN
                     IFENIRIYTYFGSVSVSILSVFLFGAGPGADFASSLLAVIFGCFVFSVAYSSVFLPLL
                     YVYTDPAEFHILWREYLDKISSKFHR"
BASE COUNT          809 a          660 c          632 g         1003 t
ORIGIN      
        1 ccctgtatgc atactacagg aaaaagagaa aacaatgtct tcagtttagt ctctaaaagt
       61 ctcttaaaat ataccataac ttgatatttg ggaatagtat tcctgaaaat caagtcagaa
      121 attctttttt cttcacaaat ttggatatac ttgacctttg ttgagaaata cagcagtaag
      181 gcataacaaa acagtttaaa aacttttgag gcggaaatgc cgactattgg ggatgtttaa
      241 catccgactt tccggcatta ttccagaacc ttactgcttc ttccagttca atatccttcc
      301 tgaactgtaa agcacatcca gatatgtttt ctgaagaatg aactttgctt ccagaagtct
      361 gttctgggca tcgaaccagt ttctctgtgc gtccagatag tcaatgatct gagtccctcc
      421 tttcacatat ccgtatcgta ctgtctgcag gattttttcg gaggtctcaa aaattttctg
      481 tattctttta taattaattt ttgctgtaga aaatgactca atggaattgt ccacttctgc
      541 gaggattgtc tttttcagac cgtcggtcag atattctgct ttttccagat ccgcctttgc
      601 ctgaagaatt tccccctgat ttctgttgtt tacagggata ggaatattta tgtacgttcc
      661 gtaaaaatct cttccgttct gaggattggc cacaaatccg aattcgggca tagggtaggc
      721 cctggcattc tgcagacgga tatttgcctt tgcgacagat tctgtactga ttctgtactg
      781 atagtccggg cgcagatcaa atgatttttt tttcagatca tctgaattga tttcctgatc
      841 gggaagtgca ggaagttctt ttgagtcaat ggtgaagtcc ccctgttcat atcctaagag
      901 tgcccggaga ttccgtcttt catttttgta ttcctgctct gcagaaatca gagtggcttc
      961 atatgtttcc acgaggttta aagtacggaa atactctgcc tgagtgatga cctgattttt
     1021 catcctgagt tcgtttactt tcagaagctc cttggagaag tctgcagttc ttcggattac
     1081 actcagtctt tccagagtaa accagacatc catccatttg gacgcagtga tgtacagaac
     1141 gttccgttca aactcttcca cgtttctctg ttctgcttcc agtgatttta atgctctttc
     1201 gattgcatag gttctctgtc ctgcgaccgg tataggctgt gagatctgaa aataaccttg
     1261 agtattctga ggggaaaatc tggaagatcc aggatcactg tggggactga ccacaagctg
     1321 attgttgaaa acaggattcg gatacagcct ggatgtaact acatctccga ctgccttttt
     1381 tacgctcatc cgctctgcca gaagtttagg attctgcttt cttgctatct ccattacagc
     1441 ccagatatcc agcttcccgt tgagttcatc tgcgaaaatc gaaaagcaga atgtgagact
     1501 aacgatgaaa tttgctgctg atcttatcaa gatattctct ccataggata tgaaattctg
     1561 cagggtcggt atacacataa agcagaggca gaaacacaga actgtatgca acagaaaaaa
     1621 caaaacatcc gaaaatgact gcaagaagag aggatgcaaa atctgcacca ggtccggctc
     1681 cgaaaagaaa caccgaaaga attgaaacgg acactgatcc gaaatacgta taaattctta
     1741 tgttttcaaa tatgttcagt ttaatgataa gataattatc catattgtgc tcagaagctt
     1801 ccatcagtgt gttcataaca aggatgggca gaaacattcc cagtccggat gaaagtatga
     1861 ggccggcata cgaggaagtg tcccagttgg atccgaacag aagaagtatg attccgcttc
     1921 ctgcgactga aaaaatggag cctgcaaaaa tcaggattac gtatctcaga ttcatctggt
     1981 aaatgaccag cagcacaatg aaaagcacca taaagagaag aggaatcgtt tcggaaagtt
     2041 ttgattccag tcgtttcagg tttccatact gtccgcctgc attgaaatat ctggatttgt
     2101 caccgtgttt ttctcttatt ttggcagtca gtttttccgt aatataactt atatttcttt
     2161 ttttcagggt ggctctcact gtatacagtc tttctccgtt ttctctgtaa atgactgatt
     2221 ctccttcttc cagtcgaatg tctgcaattc tctgaagagg gattctctgt ccggattttc
     2281 ccgggatcag tatttttccg agagcctccg tcgtgcttct gatttctttt gtatatttca
     2341 ttacaatatt gaactttttc tcgtcctcat aaagatacga gattggcaca cccgcgatgg
     2401 ctgtcttcag aacatcgttg atgtcgctga cattgatccc cagccgggcc gattcttctc
     2461 tgttgattct gatttttact actgactgtc tgccttcctg ttcaatgtat gtttcctcca
     2521 gttctccata ctgtcttact gcctcaaggg tgtcttctgc aaactttctg tcgatccctg
     2581 tctttgcata aatgtctatt gctatgtccg ctgaggttcc ggatgcattt tccatgagag
     2641 tgtctctgat cggctgtgtc atactgaatg ccattccggg gaaattgttc ttcagtattt
     2701 cgctgatctg accggatatt ttcgagaaat ccgtaaagac gcttcttttc agatttacaa
     2761 ggatttccat tctgttgaat ccgtatggat ctgttccatt gtcgtttctt ccgagctgag
     2821 tcacaatctg ctcaacgtct gggatcagtg aaatatgctc tcggattttt tcgggataaa
     2881 tttttgctgc ttcgagagat attccaggtg aaagaaaaac tctgatccag agacttccct
     2941 cgttcagtgc aggaaagaag tttcgtcctt tcagaaatga gaaagatgat attatcattg
     3001 tacatataaa tacggacatc agtacgctgg tctgttttcc ggaaagaatg tccattgcct
     3061 tgttgtacca ggagactcct gaaagaatga tttcagtttc cagg
//
LOCUS       DAOQGJ010000234         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18603_length_3103_cov_3.418635, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000234 DAOQGJ010000000
VERSION     DAOQGJ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18603_length_3103_cov_3.418635"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PK683_04915"
     CDS             complement(<1..904)
                     /locus_tag="PK683_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07820.1"
                     /translation="MNKKSQAAPSELKTGDTVPDLKNIKWTGEKPAVFKKKYTAVIFW
                     NTWDDNSVNSLSEWNGLQEKYSDILTVVGLSDQKKEDMAECLRDAKSDFVSGSLPAGL
                     FSQYMEGYYMIPAVFLVSSEKKLIWKGHPFYLAKIMKILTEENGSQKEVDEFEKLRKR
                     FDNLRYAMSGNNSECLPDKEKYKSYAADYLKVFPEDSFVHNILDEFEVLIPGEKVPSL
                     KKKEWLSGEAEIERKAFLALFIEAEEDLLSLGKLHKKFHSRLPTVGLIKKSRAGDLKL
                     SRTKMPVAQISDDLYNKYMTKNTVK"
     gene            complement(1062..2333)
                     /locus_tag="PK683_04920"
     CDS             complement(1062..2333)
                     /locus_tag="PK683_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07821.1"
                     /translation="MKDVNRYPNAFLVNEEGRLLWQGNPFELSDEPNLKNFVKPEYRK
                     KIMDAADLRDRLFSLDLLGAGGKYADNPHKDEIRAHAKDCYKTFDRDEDLRILICNYD
                     HLLCGEDAPDPGKIKWVQGKSELGGKYTVITAIDCAEEYGTRISAETDRILAAFGEKI
                     SLFGISRNSADEVSQFLESRSEEIKYPVGTVSEKVYKAYVTDNTLLGESGCFLISPEK
                     KFLWYGNVSEAFSILNGLLNSGLKEKDIEELTGERDLFERLRIDLLNYKALYNSENLK
                     KLKGWGSKILKTVPNCMEVINFLLRSSASADYEDFRKICTELSTSSFSIQDYKVLLDE
                     ADKNGEGLFPYEAAPKWMSEMISLDPESADSYVFCSFYLENLHLPDKALSLAEKAVKI
                     NPDLKDELNEKISRLHRIIKAGQKSLEWIRS"
     gene            complement(2460..2654)
                     /locus_tag="PK683_04925"
     CDS             complement(2460..2654)
                     /locus_tag="PK683_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07822.1"
                     /translation="MKNNDKISFKFELKEGRTVPGLKGVKWIKGPKAEIGSELMLIEC
                     FKTDVYYDEPPYMNEIPKKY"
     gene            complement(2671..>3103)
                     /locus_tag="PK683_04930"
     CDS             complement(2671..>3103)
                     /locus_tag="PK683_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07823.1"
                     /translation="KLAKKALAVYPGNNYILDILLKEAGKIGYDEILSVCSEIDTSEF
                     NSEQLRLLFHSFDSLETAKPHEYGIKWLHQAVKLDPKSDHLWESYCDALAKLYLTDEA
                     LKAAEEGLKVTGDFRRDTLESRRDHMLRIQSGKKAAAQYFK"
BASE COUNT          820 a          724 c          549 g         1010 t
ORIGIN      
        1 cctttactgt attttttgtc atatatttat tatacagatc atcagatatt tgcgcaacag
       61 gcatttttgt tctgctcagt tttaaatctc ctgcccggga ctttttaatc agtccgacag
      121 tcggaagcct gctgtgaaat tttttatgaa gctttccaag actcagaaga tcttcttctg
      181 cttcaatgaa gagggctaga aaagcctttc tttcaatttc agcctcaccg gaaagccatt
      241 cttttttttt caggctaggc actttttctc caggaatcag tacctcaaac tcatcaagta
      301 tattgtgaac aaaactgtct tccggaaaaa ccttgagata gtccgccgca tatgacttgt
      361 acttttcctt atcaggaaga cactctgaat tattcccgga catcgcgtat ctcagattgt
      421 caaaacgttt tctcagcttt tcaaactcat ccacttcctt ctgtgaaccg ttttcttcag
      481 taagaatttt cattattttt gcaagataaa aaggatgtcc tttccaaatc agttttttct
      541 cagaactgac aagaaaaact gcaggaatca tataataacc ttccatatac tgactgaaaa
      601 gaccggcagg gagagaaccg cttacaaagt cagattttgc gtctctcaga cactctgcca
      661 tatcctcctt tttctgatca gaaagaccga ccaccgtcag tatatcactg tatttctcct
      721 gcagcccgtt ccattccgaa agggaattta ccgagttgtc atcccaggtg ttccagaaaa
      781 tgacagctgt atattttttt ttgaatacgg caggtttttc gcctgtccat ttgatattct
      841 tcagatccgg aacggtatct cctgtcttga gttctgatgg agcagcctga gattttttat
      901 tcatattcaa tcctgtcaat tttagtattc aaagaagatt gtcctgattg ggcagaaatt
      961 ctcacccttg cacaaactga tcgtcagcag acatcgattg aataacaata tgcagacata
     1021 ctgttattca attctataat ctgcctatcc aataagcgca gtcagcttct gatccattca
     1081 agacttttct gtcctgcttt aatgattcgg tgaagcctgc ttattttttc attcaattca
     1141 tcctttaaat caggattgat cttcactgct ttctcagcca gactcaaagc cttgtccggc
     1201 agatgcagat tttccagata aaaagaacag aatacataac tgtcagctga ttcaggatca
     1261 agagagatca tttcagacat ccatttagga gcagcttcat aagggaaaag cccttctccg
     1321 tttttatcgg cctcatcaag aagaaccttg tagtcctgga tactgaatga agaggtgctt
     1381 agttcagtgc agatttttct gaaatcttca tagtcggcac ttgcggaaga tcggagcaga
     1441 aaattaatga cttccataca gtttggaact gtcttcagaa ttttacttcc ccagcccttc
     1501 agcttcttaa ggttttctga attatataaa gctttataat tcagaagatc aatgcgaagt
     1561 ctttcaaaca gatccctttc gcctgtaagc tcctcaatat ctttctcctt caaacccgaa
     1621 ttcagaaggc cgttcagaat cgagaaggct tcactgacat ttccatacca gagaaacttc
     1681 ttttcaggac tgatgagaaa acatccgctt tctcccagaa gtgtattatc ggtaacataa
     1741 gctttgtaga ctttctcgga aactgtaccc acaggatatt ttatttcttc ggaacggctt
     1801 tccagaaact gagaaacttc atcagcagaa tttctgctga ttccgaaaag actgattttt
     1861 tcgccgaatg ctgccaggat tctgtctgtc tcagcggata tccttgtacc gtattcttcg
     1921 gcacagtcaa ttgcagtaat tactgtgtat tttcctccca attccgactt cccctgaacc
     1981 catttgattt ttcccggatc aggggcatct tcaccgcaca gaagatgatc ataattgcag
     2041 atcagtatgc gaagatcctc atcccgatca aatgttttgt aacagtcttt tgcatgagct
     2101 ctgatttcat ccttgtgcgg attgtctgca tatttcccgc ctgcacccag aagatccagt
     2161 gaaaagagcc ggtctcttag atcagctgca tccataattt tttttctgta ttcgggcttc
     2221 acaaaatttt tcagattcgg ttcatcactc agctcaaaag gatttccctg ccagagaagt
     2281 ctcccttctt catttaccag gaatgcattc ggatatctgt tgacatcctt catatatgaa
     2341 tcgtagaggt cctgagagca gatgcctaca ggataaaaaa ctgtatctcc tctttcggaa
     2401 aggaactctt caacggtctc cttgtcttca gaggtaatag ctgcacagac tattttttct
     2461 cagtattttt tgggaatctc attcatgtac ggaggttcat cataatatac atctgtcttg
     2521 aagcattcaa tcagcatcag ctctgatccg atttccgcct taggcccctt tatccatttc
     2581 actcctttca gacccgggac agtccgtccc tccttgagtt cgaacttgaa agatatttta
     2641 tcattatttt tcattattta atcctttatc tcacttgaaa tactgtgcag cagccttttt
     2701 tcctgactga atacgaagca tatgatctct tctggattcc agcgtatccc gacgaaaatc
     2761 tccggtcact ttcagtcctt cctctgctgc tttcagcgct tcatcagtca gatacagctt
     2821 ggcaagcgca tcgcagtatg attcccataa gtgatcagat ttcggatcca gttttaccgc
     2881 ctgatggagc catttgatac cgtactcatg cggcttggcc gtttccagag aatcgaatga
     2941 atggaaaaga agtctgagct gttccgagtt aaattctgat gtgtctattt cactgcatac
     3001 agagagaatt tcatcataac cgatctttcc tgcttccttc agaagtatat ccagtatata
     3061 attgtttcca ggatagactg ccagtgcctt ttttgcaagt tta
//
LOCUS       DAOQGJ010000235         3097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18665_length_3097_cov_2.574622, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000235 DAOQGJ010000000
VERSION     DAOQGJ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18665_length_3097_cov_2.574622"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PK683_04935"
     CDS             <1..436
                     /locus_tag="PK683_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH07824.1"
                     /translation="RLSLIFCIAVLFSGPIWAKSNWGVYWDWTDARLQSFFALFLGLC
                     GYFLLRNLIPDVQKQAKISSVFTVFISLNALLTWGAIRWMDNPGNHPGSVLGKGGMDS
                     EMKTAFYLNVLSYHILFLILSVILYRLVRTERQNKKLLYSEN"
     gene            438..1280
                     /gene="purU"
                     /locus_tag="PK683_04940"
     CDS             438..1280
                     /gene="purU"
                     /locus_tag="PK683_04940"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNH07825.1"
                     /translation="MNSAWILRIKRKDEPGIIFRITGILSSLGANIIENEEFVDKEKS
                     LFFMRTEFEWNGEKTELEMHLSGFLYPDSEWTVDRRRTRKIWVLATKEHHCLSEILVR
                     TYHREWNAEVIGVISNHEVLRDFTEKLGFRYFYADHSGKDRKDHEKEIENILLSSEDF
                     DFIVLAKYMRILSPEFSERYSGKTVNIHHSFLPAFIGARPYRQAFERGVKIIGATAHF
                     VTPDLDQGPIISQSVVPVDHSFTEEDLIRTGRDIEVQVLVSAMKLIFEERVFIDGNKT
                     VILK"
     gene            1331..2611
                     /gene="hisD"
                     /locus_tag="PK683_04945"
     CDS             1331..2611
                     /gene="hisD"
                     /locus_tag="PK683_04945"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNH07826.1"
                     /translation="MNISIRKTSLGDDVCRGILNRAKEDLSSARDAVWPILEDVRKNG
                     DSAVRKYTEKFDGIVLSSFAVKPSEVKTEISSAEKKAFEKAKENIWNFHELQKPVNRE
                     KTVDGNRLGIRYTPIERLAVYAPGGTALYPSSVLMGVIPAKIAGVREVNLFTPPSKNG
                     LHPSLVFVAEMLGVDNIFTVGGAQAVAAAGYGTESIPKADFIVGPGNKYVAAAKGVLA
                     GLGQIGIESIAGPSEVFIIADDTANPEWIACDMLSQAEHGEDSVAVLATTSAELAERV
                     KSELSRAAVQRPKRLEMKRKAIENNSHILIFPSMEQCIDFSNQYAPEHLEIQTKDYNS
                     VFEKILHAGSVFLGPYSPVAVGDYISGTNHILPTAGLARISSSLGVDSFLKRVTYQEV
                     TKDSLMNLYPHVDLMSRIEGLDEEHGTSVKLRTL"
     gene            2611..>3097
                     /locus_tag="PK683_04950"
     CDS             2611..>3097
                     /locus_tag="PK683_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNH07827.1"
                     /translation="MKVVRKISELREAVPKGKKNGFVPTMGFLHEGHMTLVREARKKS
                     DIVVLSLFVNPAQFNSREDYDNYPRNEARDLELCKKNGVDTVYIPDPDEIYIGGKIPD
                     ISIRIPHLMKNLCATARPGHFEGVLLVIGKLFHAVRPDIVFLGKKDYQQYLIIKEFTE
                     VL"
BASE COUNT          905 a          616 c          750 g          826 t
ORIGIN      
        1 caggctgtct cttatattct gcattgcagt tcttttttcc ggaccgatct gggccaaaag
       61 caactggggt gtgtactggg actggacaga tgcgaggctt cagtctttct ttgcactctt
      121 tctgggactc tgcggatatt ttcttcttcg aaatctgatc ccggacgtac agaaacaggc
      181 aaagatttca tccgtattca ctgtatttat atctctgaat gctcttctca cctggggcgc
      241 aatcagatgg atggacaatc caggaaatca ccccggttct gttctaggca aaggagggat
      301 ggacagtgaa atgaagactg cattctatct gaacgtactt tcctatcaca tattatttct
      361 gattttatca gtcattctgt acagacttgt aaggactgaa agacagaata aaaaacttct
      421 gtactcagag aactgaaatg aattctgcct ggatactccg gatcaaaaga aaggacgagc
      481 ctggaatcat tttccgaatc acggggattc tttccagtct cggtgcaaac atcattgaga
      541 atgaggaatt tgtagataag gaaaaatccc tctttttcat gaggactgag tttgaatgga
      601 atggcgaaaa gactgaactg gagatgcatc tttcgggatt tctgtatcct gattccgaat
      661 ggactgtgga cagaaggaga accagaaaaa tctgggtgct tgcaacgaag gagcatcact
      721 gtctttccga aatactggtg agaacctatc acagggaatg gaatgctgag gtaatcggtg
      781 ttatttccaa tcatgaagtg cttcgggatt ttaccgaaaa gctgggattc cggtattttt
      841 atgcagatca ttccggaaaa gacagaaagg atcatgaaaa agagatagaa aatattcttc
      901 tttcctctga ggactttgac tttattgttc tcgcaaagta catgagaata ctcagtcctg
      961 agttttccga gagatattca ggcaaaacag tgaatatcca tcattcattt cttcctgcat
     1021 tcatcggtgc gagaccctac agacaggcat ttgaaagagg ggtgaagatc atcggagcaa
     1081 ctgcacattt tgtgactcct gacctcgatc aggggccgat catttctcag tccgtagtgc
     1141 ctgtggatca cagttttacc gaagaggatc tgattcggac aggaagggat atagaggtgc
     1201 aggtgcttgt gtccgcaatg aaactgatct ttgaagaacg ggtttttatt gacgggaaca
     1261 agactgtcat tctgaaataa accctctgat tttttctttc catttttctg cttctgcagg
     1321 gaatggaagc atgaatattt ccatccgtaa aacaagcctc ggcgatgatg tctgcagggg
     1381 aattctgaac agagcaaaag aagacttgag ttctgccaga gatgctgtct ggccgattct
     1441 ggaagatgtc agaaagaacg gcgactctgc agtcagaaaa tatactgaaa agttcgacgg
     1501 gattgtgctc tcctcatttg cagtaaaacc ctccgaagtg aaaacggaaa tctcatctgc
     1561 tgaaaaaaaa gcgttcgaga aagcaaaaga aaacatatgg aattttcatg aactccagaa
     1621 acctgtgaac agagagaaaa cggtagacgg gaacaggctc gggatcagat acactccgat
     1681 tgaacgactt gctgtgtatg ccccaggcgg aacagctctt tatccatcgt ctgttcttat
     1741 gggtgtaatc cctgcaaaaa ttgcaggggt cagggaagtg aatcttttta ctcctccctc
     1801 aaagaatgga cttcatcctt ctcttgtatt tgtggctgaa atgctcggag tggacaatat
     1861 ttttactgta gggggagcac aggcagttgc ggctgcaggt tacggaacag aatccattcc
     1921 caaggcagat tttattgtag gtcctggaaa caaatatgtc gctgcagcga agggtgtgct
     1981 tgcaggactc ggacagatag gaattgaaag cattgcaggg cccagtgaag tgttcatcat
     2041 tgcagatgat acagcaaatc cggaatggat tgcatgtgat atgctttctc aggcggagca
     2101 cggagaggat tctgttgcag tccttgccac gacgtcggcc gagctggcag aacgagtgaa
     2161 gtcggaactt tcaagagcag cagtgcagag acccaaaaga ctcgaaatga aaagaaaggc
     2221 aattgaaaac aacagtcata tactgatatt cccttccatg gaacagtgca tcgacttcag
     2281 caatcagtat gcgcctgagc acctggaaat tcagaccaag gattacaatt ctgtatttga
     2341 aaaaatcctt catgccggtt cagtgtttct tgggccctat tcacctgttg cagtcggaga
     2401 ctatatcagc ggaacaaatc atatccttcc cacggcaggg cttgcaagaa tctcttcttc
     2461 tctcggggtg gacagttttc tgaagagagt gacctatcag gaagtcacaa aggattcctt
     2521 gatgaatctg tatcctcatg tggatcttat gtcgagaata gaagggcttg atgaggaaca
     2581 cggaacttca gtaaagctga ggacactgta atgaaagttg taaggaaaat atccgaactc
     2641 agggaagcag ttccgaaagg aaaaaaaaac ggttttgtgc ctacaatggg atttctgcac
     2701 gagggacata tgactcttgt gagagaggcc cgtaaaaaat cggacattgt tgtcctctct
     2761 ctttttgtga atcctgcaca gttcaacagc agggaggatt atgacaatta tcccaggaat
     2821 gaagcccgtg atctggaact ctgcaaaaag aacggtgttg atacagtata tatacctgat
     2881 cctgatgaaa tatatatcgg aggaaaaatt ccggatatca gcatcaggat acctcatctt
     2941 atgaagaatc tctgtgcgac tgcgagaccc gggcattttg aaggagtgct tcttgtaata
     3001 ggaaaacttt ttcatgcagt ccggcctgat atcgtatttc taggcaaaaa ggattatcag
     3061 cagtatctga taataaagga atttactgag gttctga
//
LOCUS       DAOQGJ010000236         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18747_length_3088_cov_3.626113, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000236 DAOQGJ010000000
VERSION     DAOQGJ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18747_length_3088_cov_3.626113"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PK683_04955"
     CDS             complement(<1..121)
                     /locus_tag="PK683_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNH07828.1"
                     /translation="MNIYVGNLSYETTESDIAAMFEAYGQVNSAKIITDKYTGQ"
     gene            complement(520..1545)
                     /locus_tag="PK683_04960"
     CDS             complement(520..1545)
                     /locus_tag="PK683_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HNH07829.1"
                     /translation="MGNKRSKNEDLEYFYDEDFPHTDDSLLENNDNSEVEWIKAEEIF
                     EDGELFGEISTDDVSQGSMGNCWFISAIAALAEFPDLVQNLFITQEIPEDGRYELQIF
                     DLRTNEWESVEIDSYIPSLNKTPIYCTSNKNKLWPLLLEKAVAKFCGTYRNFGAYISG
                     LVFPHLTGMRTVVFSREEVEITDRWKRSTHKFDIENGINYIDGTDEKYSLEELFDLLV
                     EHNENEDIMTCSAYLYKIENDARGITASHVYSLTDAVRADDFMLLKLRNPWGYGEWEG
                     DWCDDSELWEEYPEVKEALGFEPADDGSFWISLEDFSKRFDDITVCIRGDEIEDSSEY
                     LGGDSDE"
     gene            2108..2776
                     /locus_tag="PK683_04965"
     CDS             2108..2776
                     /locus_tag="PK683_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNH07830.1"
                     /translation="MNEFINLGHAVCKKQKDETWTYALSRMNERRNNFRTEYRERFLK
                     ENKKNYSVMPLTEEEVNKIQESIGGKLPEDYRQWLLDIGYYYKLRDIAYDGENPAWIS
                     RPFHFISRYEETWNEDEDQRRLQTAVAPCFQDRIRNLPRSLEYGCFDGLLKLNGDEPF
                     HNLVVNSGDPLQTGRIWASYDYNVFITEPIEECEEFVKEYGYKGGPMDFIDYARLVLY
                     TYFN"
     gene            2895..>3088
                     /locus_tag="PK683_04970"
     CDS             2895..>3088
                     /locus_tag="PK683_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH07831.1"
                     /translation="MNVLITGAGGGFGKLTVKELAENGFSIVGTMRDIKNRNRENAAE
                     LEKLGAKVIEMDVTSSASADA"
BASE COUNT          978 a          621 c          596 g          893 t
ORIGIN      
        1 attgaccagt gtatttgtcg gtaatgattt tggcagaatt gacctgtcca taagcttcaa
       61 acatagcagc gatatcgctc tcagtggttt cgtaagatag atttcctaca taaatattca
      121 tagtgaatgt ccttaatttt tttttgactg gagcttataa agataaattc aatatttatt
      181 tgaaaaatag aaaaaatctt attataaaat ttctttgttt ttaaagataa cttcaggaga
      241 aaggcagaaa cacaaatact cttgagaaca ataatcaaag ggaaacaaat aatccaaatc
      301 aattactaca ttatttttag caggaaatca gtcaagttta tatcaaaaat ccgaactgtt
      361 tcccataaga cagcaggcag aatcttgttc aggtcaatgg gtttcttgag ggcttgcact
      421 ggcggcggga cggtgcaagg cggaatttac actgaagtac gcgagggatg cgcagaataa
      481 aaactgttga agcaccgcga gatgctgcag caccactcac tattcgtccg aatccccgcc
      541 taaatattca cttgaatcct caatttcatc ccctctgatg cagactgtta tatcatcaaa
      601 acgctttgag aaatcttcca gagaaatcca gaaggagcca tcatctgccg gttcgaaacc
      661 gagtgcttct ttaacttccg gatactcttc ccaaagttct gaatcatcgc accagtcgcc
      721 ttcccattca ccatatcccc atggattgcg taatttcagg agcataaaat catcagctct
      781 gacggcatct gtcagagaat aaacatgcga agcagtaatt cctcttgcat cattttcaat
      841 tttatacaga tatgctgaac aagtcattat atcttcattt tcattatgct caacaagaag
      901 gtcgaacaat tcttcgagac tgtatttctc gtctgttcca tcaatatagt ttatgccgtt
      961 ttcaatatcg aatttatgcg tgctccgctt ccatcggtca gtaatttcaa cttcttctct
     1021 tgaaaaaaca acggttcgca ttcctgtcag atgaggaaaa acgagtccgg aaatgtaagc
     1081 cccgaaattt ctgtatgtac cgcaaaactt tgcaacagcc ttttcgagaa gcaaaggcca
     1141 cagtttgttt ttatttgaag tacagtatat tggagtttta ttcaatgatg gaatgtaaga
     1201 atcaatttca acactctccc attcattggt tcttaaatca aagatctgaa gttcgtacct
     1261 gccgtcctcc ggaatctcct gtgtaataaa aaggttttgc actaaatcag gaaattcagc
     1321 taaggccgca atcgcactga taaaccagca atttcccatt gaaccctgac ttacatcatc
     1381 cgttgaaatt tcaccgaaca gctcgccgtc ttcaaatatc tcttctgcct ttatccattc
     1441 cacttcactg ttatcattat tttccaaaag tgaatcatca gtatgcggaa aatcctcatc
     1501 ataaaagtat tccagatctt cattttttga acgtttattt cccaaattgc atcctccaaa
     1561 atcataaaaa taaacagctt tattttatcc catttttatc agttcgatca aagattcaac
     1621 tgttaagtgc ataaggtatg cttgacgagt aaagacttca taaagtgttt gcagaatatt
     1681 tttttcagca cttacggtct cgcttttccg ccaagagtcc tgatctgatt ttaaatcaga
     1741 gctgtcaaca ggctttatcg tcacttattg acttctccat atccgactgc tggcgaattg
     1801 acgtcagctt cagagtctgc agttgcactg agtccgtcaa tcggtgactg cagcaagtct
     1861 tttttgacca gactttatga tgaaattaat ttaatcaact tatttattat caactttatt
     1921 tcaattgata atgaaactct gtttccgatt tgacagatga ccgcccaagc atccgttcat
     1981 tggacggaaa acttgcctgg aaaaatcggc ttgcagtatt tccatctgtc tgccagtatc
     2041 agactgacac aggaaaccta tgcgggaaaa acagaaaaat ccgaatcatt cagaaacaga
     2101 taatcaaatg aatgaattta ttaatcttgg ccatgcagtc tgtaaaaaac agaaagatga
     2161 aacctggacc tatgctctca gtcgaatgaa tgaaagaagg aataattttc ggacagaata
     2221 cagggaaaga tttctgaaag aaaataaaaa aaattattct gtaatgcctc tcactgaaga
     2281 ggaggtgaat aaaattcagg aaagcatagg aggaaaacta ccggaagatt acagacagtg
     2341 gcttctcgat atcggatatt attataaatt aagagatatt gcttatgacg gagaaaatcc
     2401 agcgtggatt tcaagaccct ttcatttcat cagcagatac gaagagacct ggaacgagga
     2461 tgaagatcag aggaggctgc agacagccgt tgctccctgt tttcaggatc ggatcagaaa
     2521 tcttcccaga tcgctcgaat acggatgttt cgacggtctt ctgaaactga acggtgatga
     2581 acctttccat aatctggtgg tgaattcagg tgatcccttg cagacaggac gcatctgggc
     2641 atcttatgat tacaatgtat tcattactga accgatagaa gaatgtgaag agttcgtgaa
     2701 ggaatacgga tacaaaggcg gtcccatgga tttcattgat tatgcgaggc tcgttctgta
     2761 tacatatttt aattaatgcc tgtctgaatg aagaacaggt gcggcacgga gataaaagca
     2821 ctcagccgca aaaaactgat tttattcttg actaaattac aaataacaaa atatgtaact
     2881 tgtaaggaga aattatgaat gtactgatta caggcgcggg cgggggcttt ggaaagctta
     2941 cagtaaagga acttgcagaa aacggctttt cgattgtcgg aacgatgcgc gacataaaga
     3001 accgaaacag ggaaaatgct gcagagctgg aaaagctggg tgcaaaagtc atcgagatgg
     3061 acgtgacttc ttcagccagt gcggatgc
//
LOCUS       DAOQGJ010000237         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18801_length_3083_cov_3.534676, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000237 DAOQGJ010000000
VERSION     DAOQGJ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18801_length_3083_cov_3.534676"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PK683_04975"
     CDS             complement(<1..1395)
                     /locus_tag="PK683_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNH07832.1"
                     /translation="MIFSGKLNFFILFLFSFTLSAEVSVGLSKKSIKKGEPLYLIVEA
                     NGGESVENRRHIHSSKGVTAEFIGTEQSYSMINGKSSVKKILKYRVVTSKDGALETPP
                     IQMSVNGQDKLIDPIAFSVSKEKYAPQISEDDIESPFDDLIPPWMKGKKKTAYTEPSE
                     DDLVVMFETTRDKVYLGETVVGYYNLYYKNVKKPLMERMEAQSPSFPYFGFEYLSEAG
                     VSVTDSRGVYRGKEYFLAPYQRDIFALTPLRNGKFRLGETNFTVEGSPASYFSARSVP
                     AIPKTVTVMSLPSPSPSDFTGEVGEYTASVSLGAQEFHLGESVPILLKIKGKGSGTLI
                     RNPLEKFCTERKCEGNLVFSGDMKKKQFAEQGKGVYGFNSDMEFRYSFLPERTGEYHL
                     GEAKIGFFNPYAEKYEAAVIEMPKFTVQEKRIMEKGPVSSERSNIFLYLFYILSLSSI
                     AFLGYRFRDIILNSF"
     gene            complement(1379..2077)
                     /locus_tag="PK683_04980"
     CDS             complement(1379..2077)
                     /locus_tag="PK683_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH07833.1"
                     /translation="MNYIFFALLCILSVSSAYAVDIDPAGAKIKQGVERYKSGDFPGS
                     LDSFESASRDFKEDRRLDFNKGTAQFKSGDFKSALRNFESAAASDDKSLKTESFYNLG
                     NTYNQMGDKKSAIKSYMRALKENPDFAPARKNLELIRRKEEKPPEDKDRQDDKEDKQN
                     PQNSSGSPKKDKRSGKEKHKDRAEEEQLSAKDQEKRSLSREEAERILESARQDKIRRK
                     KAQSRRPNSNDIFW"
     gene            complement(2079..>3083)
                     /locus_tag="PK683_04985"
     CDS             complement(2079..>3083)
                     /locus_tag="PK683_04985"
                     /inference="COORDINATES: protein motif:HMM:NF033566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC20035 family adhesin"
                     /protein_id="HNH07834.1"
                     /translation="VLLKETEFKNDEKNGLEKKYTKTKEVIEESNYVNNLLDGPHYKK
                     TREGRKEVEGQYSQDKKTGEWREYFTDKAMNGLKTVTNYREDKKHGKETRYYDDGKTL
                     LAEGNYENNLESGPWKFYYDTGSIEYDGAFKPVEPAPAKTAAKDETASGKTAAEPTPA
                     DELKTKAKRSGLWKRYYKSGDVFSEGNMDGGPTGEWKFYHKGGVLGAKGKMSNDLMMT
                     EGEWFDESGRPHVKGKFLISILNLDKKTDSFERKFRPSKPFIVYKEGKKYLEVKEAEE
                     KGKTIAWLFGDNGNKIGEGPIEPNTQKKHGCWTVNGKKVFYLMDSEKGGVMAQMQKCE
                     "
BASE COUNT          713 a          767 c          578 g         1025 t
ORIGIN      
        1 aaatgaattc agaatgatat cccgaaacct gtatcccaga aaggcaatac tgcttaatga
       61 aaggatataa aataaataaa gaaatatatt tgaccgttca gaactgacag gtcccttttc
      121 catgattctt ttttcctgaa cggtgaattt cggcatctcg atgactgccg cctcatattt
      181 ttccgcatag ggattaaaaa agccgatctt tgcctcgccc agatgatact cacccgttct
      241 ttccggcaga aaagaatatc tgaattccat atccgagttg aatccataca cccccttccc
      301 ctgttctgca aactgctttt tcttcatgtc ccccgaaaat accagattcc cctcgcattt
      361 tctctcagtg cagaattttt ccagaggatt gcggatcagg gttccgcttc cctttccttt
      421 tattttcaga aggataggca cagattcccc cagatgaaat tcctgagcgc ccagtgagac
      481 cgacgccgta tactcaccga cttcacctgt aaaatcagag ggtgagggag aaggaagcga
      541 cattacagtc actgtctttg gaatggccgg cactgatctt gctgaaaaat aggaagcagg
      601 acttccttct acagtaaaat tggtttcacc gagtctgaac ttgccgttgc ggagtggggt
      661 aagtgcaaat atgtctctct gatacggggc aagaaaatat tcctttcctc tgtaaacccc
      721 tctgctgtca gtcacagaga ctcccgcctc agaaagatat tcaaatccaa aatatggaaa
      781 ggaaggagac tgggcctcca ttctttccat taaaggtttc tttacatttt tataatataa
      841 attataataa ccgacaactg tttctcccag atacactttg tctctggttg tttcaaacat
      901 gacgacaaga tcgtcttctg acggttcagt gtaggcagtt ttcttttttc ctttcatcca
      961 gggagggatc agatcgtcaa aaggactttc aatgtcatcc tctgaaatct ggggtgcata
     1021 tttttccttg gagacactga aggctatcgg atcaatcagc ttatcctgtc cgtttacgct
     1081 catctgaatg gggggagttt ccagtgcgcc gtctttagat gtcacaaccc tgtatttcag
     1141 aatttttttg acactggatt taccgttgat catggagtaa ctctgctcag tcccgatgaa
     1201 ctctgcagtc accccttttg aagaatgaat atgacgcctg ttttctacgc tttctccgcc
     1261 gtttgcttcc acgatcagat ataaaggttc tcctttcttt atgcttttct tgctgagtcc
     1321 gacggagacc tctgcagaaa gagtgaaact gaacagaaac agtataaaaa agttcagctt
     1381 accagaaaat atcattgctg ttcggcctcc tgctctgagc cttttttctg cggattttat
     1441 cctgtcttgc ggattccaga atccgttctg cttcttccct gctgagagat cttttttcct
     1501 gatcctttgc cgaaagctgc tcttcttcag cacggtcctt atgcttttct tttcctgacc
     1561 ttttgtcctt tttcggagac cctgaagaat tctgcggatt ctgcttatcc tccttgtcat
     1621 cctgcctgtc cttgtcctca ggaggttttt cttccttcct tcgaatcagt tccaagtttt
     1681 tccttgcagg ggcaaaatcg gggttttcct tcagtgcacg catataggat tttatggcag
     1741 atttcttatc tcccatttga ttatatgtat tgcctaaatt ataaaaactt tctgttttta
     1801 gagacttgtc atctgaggca gccgcactct caaaatttct gagagcactt ttaaaatcac
     1861 cggatttgaa ctgggcagtt cctttattga aatccagtct tctgtcttcc ttgaaatccc
     1921 tggatgcaga ttcaaatgaa tccagagacc cgggaaaatc gccggacttg tatctttcca
     1981 ctccctgctt aatcttggct ccagcaggat caatgtccac tgcataggca gatgagacag
     2041 aaaggataca aagcagcgca aaaaaaatat aattcatcct attcacattt ctgcatctgg
     2101 gccatgactc cgcccttttc ggaatccatc agatagaaaa ctttcttgcc gtttacagtc
     2161 cagcatccgt gttttttctg agtgttcggt tcaatcggac cttctccgat tttgtttcca
     2221 ttgtctccga aaagccaggc aattgtcttg cctttctcct ctgcttcctt cacttccaga
     2281 tacttctttc cttccttata gactatgaaa ggcttggaag gtctgaattt tctttcaaaa
     2341 ctgtccgttt ttttatccag attcagaatg gagatcagga actttccttt tacatggggt
     2401 ctgccgcttt catcaaacca ttccccttca gtcatcatca ggtcgtttga catttttcct
     2461 tttgcaccga gaacaccgcc cttatgatag aacttccatt ctccggtcgg tcccccgtcc
     2521 atatttcctt cactgaaaac atcccctgac ttgtagtatc ttttccagag accggatctt
     2581 tttgcctttg tcttcagttc atctgcaggc gtaggttcgg cggctgtctt tccagatgca
     2641 gtctcgtctt tagcagctgt ttttgcagga gccggttcaa cgggcttgaa tgcaccgtca
     2701 tactctatac ttccggtatc ataataaaac ttccacgggc ctgattccag attgttctca
     2761 taattccctt ctgccagaag tgtcttccca tcgtcgtaat aacgggtctc tttcccgtgt
     2821 tttttgtctt ctctgtaatt ggtgacagtc ttcagcccgt tcatcgcttt gtcagtgaag
     2881 tattctctcc attcgcctgt tttcttgtcc tgactgtact gaccttcgac ttccttgcgt
     2941 ccttccctgg tctttttata atgaggtcca tccagaagat tgttcacata attggattct
     3001 tcaatgactt ccttagtctt tgtgtatttt ttctccagtc cgttcttctc atcatttttg
     3061 aattctgttt ccttcagaag aac
//
LOCUS       DAOQGJ010000238         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18849_length_3079_cov_2.952381, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000238 DAOQGJ010000000
VERSION     DAOQGJ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18849_length_3079_cov_2.952381"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PK683_04990"
     CDS             <1..717
                     /locus_tag="PK683_04990"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH07835.1"
                     /translation="RRGMTPLHAAAVNGHSEIVKMLIREKANVNTFDKKKISPLMGCI
                     ERGHYDVARVLLENGADPNVKNKDGQTPLHVAAANGHLDTVKILVDKGASLNAKDEEW
                     NPPVLRAVSEGHLETVAFLVRNGADINQKNRAGHSPIQLATQNGLGDLTEYISKNKDQ
                     FSTKKEVGIGEVSSFEEGTAVVKLSTKEKISENYVIYFVNEKNEEMGRGKISFITSKM
                     IKVKLESGITFKGDKALYYK"
     gene            836..>1035
                     /locus_tag="PK683_04995"
     CDS             836..>1035
                     /locus_tag="PK683_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07836.1"
                     /translation="MQIQGASYCLASKTHYQGTVPSLEEAKVIMNDPELKQHFSPRSI
                     RMAIAYGIHNDIKTFAKKYKTS"
     assembly_gap    1036..1135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1136..1946
                     /locus_tag="PK683_05000"
     CDS             <1136..1946
                     /locus_tag="PK683_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07837.1"
                     /translation="DSKDSKDSKDSKDSKDSKDSKIERKTKSAKKAEPKQNASAEISN
                     PVQSTSSEQTALETKISKRLQLAQLDLNATASEIRCYVDRFTEIVAMMQEAEDDIVQS
                     NTLYAIMSGAIAAILDGSTVYDTAGNQKIILAGGLLVSYFSYRAFRPDVTVEFKPKSS
                     NLKDLWFNVESSPNFSTSLWFLLTKPMLGEEQTIRDLLIQRWKDNGFLGADDTEERKH
                     YENLFFGAGGVSSLTVIKNRKEMNNQLQTLIQLLEQDVKVLQIELLRLQSD"
     gene            complement(1976..>3079)
                     /locus_tag="PK683_05005"
     CDS             complement(1976..>3079)
                     /locus_tag="PK683_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNH07838.1"
                     /translation="AYCSHLSGGIDSSLIAALAARFGKQKLHCFTVSFPGWKYDEASL
                     AEKTAQHIGANFTAVAVTANDLSEVLSDAVYFSEGNAINSHLAAKFILNRTVRKEGYK
                     IALTGEGSDEILAGYLHLLTDLKGRTESSDRGVLGIHVPAEDLTEIPEFQTALGFTPS
                     FIQAKILSGRKIRSLLTDEFKNLFSEEQIFRDFTNSLNIKTYISDRNKADQSAYIWIK
                     FTLANYILKTLGDGTEMAHGIEGRVPFLDHILAESAVSIPISLKIRDGVQKHVLRQTA
                     RKFLPIDVTERPKQAFMAPPFSLLNDRRGIEFAMDCFQSADFRNMGIFDSEKIMKMAY
                     KISQKTPEEQTAEEPCIMLPLTACLAGNRFHLS"
BASE COUNT          875 a          634 c          677 g          793 t
ORIGIN      
        1 aggaggggga tgacccctct tcatgccgca gcagtcaacg gccattcgga aattgtaaag
       61 atgctcatac gtgaaaaggc gaatgtgaat acattcgaca agaagaaaat ttcgcctctg
      121 atgggctgca ttgaaagagg acattatgat gttgccagag tccttctgga aaacggtgct
      181 gatccgaatg tgaagaacaa ggatggacag actcctctgc atgtcgccgc cgcaaacggt
      241 catctggata ccgtgaaaat tctggtggac aaaggcgcat ccctgaatgc aaaggatgag
      301 gaatggaacc ctccggtact ccgtgctgta tcggaaggtc atttggagac agtcgcattt
      361 cttgtcagaa acggcgcaga catcaatcag aaaaacaggg cagggcattc tccgattcag
      421 ctggcaactc agaacggtct tggagatctg acagaatata tttccaagaa caaggatcag
      481 ttttctacca agaaggaagt gggaatcggt gaggtcagtt cctttgaaga aggcacagct
      541 gttgtgaaac tgagcacaaa agaaaaaatt tctgaaaatt atgtaattta ttttgtaaat
      601 gaaaagaacg aagaaatggg aagagggaaa atcagcttca ttacttctaa aatgattaag
      661 gtaaagcttg aatcaggaat cacattcaag ggagataagg cgctttatta taaataactc
      721 agactgaact gatattttct cgacatcatt tgttcagact gaaactttgt cccccatgaa
      781 agttttcgtt ttattactgt tgtttgtttt ttcctcctgt gcaagggagg tcagaatgca
      841 gattcaggga gcctcttact gcctggcttc caagacccat tatcagggta ctgttcccag
      901 tcttgaagag gccaaggtca ttatgaacga tcctgaactg aagcagcatt tttcacccag
      961 atcaattcgg atggcaatcg catacggaat tcataatgat atcaagactt ttgctaaaaa
     1021 atacaagaca tccaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagaca
     1141 gcaaagacag caaagacagc aaagacagca aagacagcaa agacagcaaa gacagcaaaa
     1201 tcgagcgtaa aactaaatca gcaaaaaaag cagaaccgaa gcagaatgcc tctgcagaga
     1261 tttctaatcc ggtacaaagc acctcttctg aacagactgc actggaaact aagatttcca
     1321 aaagactgca gcttgcgcag ctggatctga atgcgactgc atctgaaata agatgttacg
     1381 tagacaggtt tactgagatc gttgccatga tgcaggaagc agaagacgat atagtgcaga
     1441 gcaatacact gtatgccatt atgtccggtg cgattgctgc aattttagac ggatccaccg
     1501 tatacgatac tgcgggcaat cagaagatta ttctcgcagg aggacttctt gtttcttatt
     1561 tcagctacag ggctttcaga cctgacgtca cggtggaatt caaacccaag tcatccaacc
     1621 tgaaggatct ttggttcaat gtagaaagct caccaaattt ctccacatcg ttatggttcc
     1681 ttctgaccaa acctatgctg ggagaagaac agacgatcag ggatcttttg attcagagat
     1741 ggaaggataa tggatttctg ggagcagatg atacagaaga aagaaagcat tacgaaaatc
     1801 tgtttttcgg agcaggggga gtcagttctc tgaccgtaat caaaaacagg aaagagatga
     1861 acaatcagct tcagacctta attcagcttc tggaacagga cgtaaaagtt cttcagattg
     1921 aacttctgag gctgcagtca gactgaacat tcagtctgcg gtttcattca gatcctcagg
     1981 ataaatgaaa cctgtttccc gccaggcagg ctgtgagagg aagcataatg cagggttcct
     2041 ccgcagtctg ctcctccggt gttttctgtg atattttata tgccattttc attattttct
     2101 ctgagtcaaa tattcccata tttctgaaat cggcactctg aaagcagtcc attgcgaatt
     2161 cgattcctct tctgtcattc aaaaggctga acggaggagc cataaaagcc tgtttgggtc
     2221 tttctgttac atctatcggc aggaatttcc ttgctgtctg tcgcagtaca tgcttctgaa
     2281 ctccgtcccg gattttcagt gaaatcggga tactgacggc tgattctgcg aggatatgat
     2341 ccagaaacgg aactcttcct tcaattccgt gcgccatttc tgtgccgtcg cccagtgttt
     2401 tcagaatgta gtttgccagc gtgaacttaa tccagatata tgcggactga tccgccttgt
     2461 ttctgtcact gatatatgtt tttatattca atgaattcgt aaaatctctg aatatctgct
     2521 cttctgaaaa caggttctta aactcatccg tcagcagaga acgaattttc ctgcctgaaa
     2581 gaatctttgc ctgaatgaag gatggagtga atccgagagc tgtctgaaat tcaggaatct
     2641 cagtcaggtc ttctgcgggt acatgaatcc cgagcacgcc tctgtcagaa ctctctgtcc
     2701 tgcctttcag atcagtgagc agatgcagat atcctgcaag gatttcatcc gagccttctc
     2761 cggtgagtgc aatcttatat ccttcttttc ggactgttct gttcagtata aattttgctg
     2821 caagatgcga gttgattgca tttccttcgc tgaaatacac tgcatcggac agaacttccg
     2881 aaagatcatt tgcagttacg gcaactgctg taaaattcgc accgatatgc tgtgctgttt
     2941 tttccgcaag agaagcctca tcatatttcc atccaggaaa agacactgta aaacagtgaa
     3001 gtttctgttt tccgaaccgg gctgcaagcg ctgcgatgag agaagagtct attccgccgc
     3061 tcagatgaga gcagtatgc
//
LOCUS       DAOQGJ010000239         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18896_length_3073_cov_4.044732, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000239 DAOQGJ010000000
VERSION     DAOQGJ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18896_length_3073_cov_4.044732"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..453)
                     /locus_tag="PK683_05010"
     CDS             complement(100..453)
                     /locus_tag="PK683_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07839.1"
                     /translation="MENMDTLEVYTVLKKAKFTELQSKELANLLGKYYESEPASKQDL
                     SETESRMKLELKNTELRLQKEIEQIRLETQGVKLEIQDIRLEIQTVKADLIRWMIGTV
                     FGAAGLLGIFLKYIK"
     gene            complement(752..2050)
                     /locus_tag="PK683_05015"
     CDS             complement(752..2050)
                     /locus_tag="PK683_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNH07840.1"
                     /translation="MYSDLHNHLYGCLSAETLWEIGRKNRHPKWERFIPEYKKVFGKE
                     INPDSFFETYSRPEKLGELYWFRKPGPFAEFQCSFNLIIALSVFDEEEIYEAARRTAI
                     EHYHDGVSYAEYRLMYSPAAGEADYVRKSLSACRGLKKAEEESGGKFQGRLAVSLYRN
                     SYWKEYEWLKKLQDSDNTAKQYITGIDFCFIEEGFPPKEKKEFFLKVKEDNILKPDRA
                     LAILYHVGESFQDKSALSSCRWVIESAESGAHRLGHCIALGIRPEIYRDRKITESAEE
                     RRDQIRFMLKNYEEIRSFGPVSERCELEKELEDITSLKNDEKINYHYSQKNMAELETF
                     QKFGMERIKKTDAVIESCPTSNLLIGMITEKKDLPVFRFIENGMKLVLGSDDPGIFRT
                     DLKSEFKILSECGVRAETLERIRQNGFLYKSENLSGRKNG"
     gene            complement(2051..>3073)
                     /locus_tag="PK683_05020"
     CDS             complement(2051..>3073)
                     /locus_tag="PK683_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07841.1"
                     /translation="PKQFSDLFLEYSESYLNVMYNFAAELRNDYPDWKADITVYSDCR
                     EYAEILKKKGNTKSPLLFHSEGKLWNRPVLSACYSSPKFFLSSFRHSSAEKIAAKLPY
                     SLRQAVSIYAENSIFIADDAYTVHQMNDLYLLRISEAKEIPKLSDFLKSGKKEWKKNR
                     EIYFPYAWALSVYIIDYSREGKNLFKTPEKISSLETDFKAWLSSLKFPAGYDFYLEYR
                     EKNVFTEKKKTLLLALEQNPDSCIYNKALSDIHLGEQNWKESLFYAERTLLFNFTDTG
                     AVLNALKSSIRLQEYGKAEYYLFLMDSWKKSFTERENFSQEIGRWRGRNSDAKYFGPE
                     LIFWEK"
BASE COUNT          793 a          664 c          578 g         1038 t
ORIGIN      
        1 cgtatcgagg tgggcggtga aaatcactcg gtaacctgct tttacacagt cccattttct
       61 aatagtccct cggctgtcag aatggcggcc catggttttt tattttatat attttaaaaa
      121 tattcccaga aggcctgctg caccgaacac agtcccgatc atccatcgga tcagatctgc
      181 tttaactgtc tgaatttcca gtctgatatc ctgaatttcc aatttgactc cctgggtttc
      241 cagtctgatc tgttcaattt ctttctgaag ccgaagttct gtatttttta attccagctt
      301 catcctgctt tcggtttcac tgagatcctg cttgcttgcc ggctctgatt catagtattt
      361 cccaagcaga tttgccagtt cctttgactg cagttctgtg aactttgctt tcttcaggac
      421 agtgtacact tcgagtgtat ccatgttttc catatatata cactgtttca aaaagtgtca
      481 agaattgaaa ttttctgttg agatattcac ctttatactg gttttcaaaa gtctttgcgt
      541 atttatcttt cagaactcag taaaatcaag cctgccggct gggaaaagcg caattattta
      601 tgaaaatcag tataacaaaa ataaattcca aaggtgaggt gaccgtcagt ccaatgttat
      661 tttttacaga aaaatgaagg acattatacc cagactgcag agctgcactg tttgcggtaa
      721 ttgatttggg cagacaaatg aagcaggagt cttaaccgtt cttccttcct gaaagatttt
      781 ctgacttgta aagaaatccg ttctgtctga tcctttccag tgtttcagca cggactccgc
      841 attctgagag aattttaaat tctgacttca agtcagtccg gaatattccc ggatcatctg
      901 aacccagtac cagtttcatt ccattttcaa taaaccggaa tacaggcaga tctttttttt
      961 cagtaatcat tccgatgaga agattggaag tgggacagga ttcaatgact gcatctgttt
     1021 tttttattct ttccattccg aatttttgaa atgtttcaag ttccgccatg tttttttggc
     1081 tataatgata atttattttt tcatcatttt ttagtgatgt aatatcctca agttccttct
     1141 cgagttcaca cctttctgaa acaggtccga aggatctgat ttcctcgtag tttttcagca
     1201 taaaacggat ctgatctctt ctttcttctg cagattcagt gatctttctg tccctgtata
     1261 tctcaggtct gattccaaga gcaatgcagt gaccaagcct gtgtgcgcct gactctgcag
     1321 attcaatcac ccatctgcag gaagaaaggg cagacttgtc ctggaaactc tctcctacat
     1381 gataaagtat tgcgagggct ctatcgggtt tgaggatatt gtcctccttg actttcagaa
     1441 aaaactcttt tttttcctta ggcggaaatc cttcttcaat gaagcagaaa tcaattcctg
     1501 tgatatactg cttcgcagta ttgtccgaat cctgcagttt tttcagccac tcatactctt
     1561 tccagtaaga attcctgtac aatgaaacgg ccagccttcc ctggaatttg ccaccgcttt
     1621 cttcctctgc ctttttcagt cctctgcagg cacttaagga ttttctgaca taatcggctt
     1681 ctccggctgc aggagaatac attaatctgt attctgcata cgatacccca tcatgataat
     1741 gctcaatagc agtccttctg gctgcctcat atatttcttc ttcatcaaag actgaaagtg
     1801 cgataatcag attgaagctg cattgaaatt cagcaaacgg tcccggtttt ctgaaccagt
     1861 aaagctctcc cagtttttca ggtctgctgt atgtttcaaa aaatgaatca ggatttattt
     1921 ccttgccgaa gactttttta tactcaggaa taaatctttc ccatttggga tgtctgttct
     1981 tccttccgat ttcccatagg gtttcagcag aaaggcatcc gtataaatga ttatgaagat
     2041 cgctgtacat ttatttctcc cagaatatga gttcaggccc gaaatacttt gcgtcagaat
     2101 tccttcctct ccatctgccg atttcctggc tgaagttttc tctttcagtg aaactttttt
     2161 tccaggagtc catcaggaaa aggtaatatt cagcctttcc gtactcctga agccttatac
     2221 ttgatttcag tgcattcagt acagctccag tatcggtaaa attgaaaagg agagttcttt
     2281 ctgcataaaa cagggattct ttccagttct gttccccaag atgaatatct gacagagcct
     2341 tattatagat gcaactgtcc ggattctgtt ccagtgcaag aagcagtgtc ttcttttttt
     2401 ctgtgaaaac attcttctcc ctgtattcca gatagaagtc atacccggca ggaaatttga
     2461 gagagctcag ccaggctttg aagtccgttt caagtgagct tattttttca ggagtcttga
     2521 aaagattttt tccttcccgg ctgtagtcta tgatgtatac tgagagagcc catgcgtatg
     2581 ggaaataaat ttccctattt tttttccatt ctttttttcc cgatttcaga aagtcagaaa
     2641 gttttggaat ctctttggcc tctgaaatac gcagaagata gagatcattc atctggtgca
     2701 ctgtataagc atcatctgca atgaatatgg aattttctgc ataaatggaa actgcctgac
     2761 ggagagaata cggaagtttt gccgcaattt tctcagcact gctgtgacgg aatgatgaca
     2821 ggaaaaattt tgggctggag taacatgcag acaataccgg tctgttccag agttttcctt
     2881 ctgagtgaaa cagaagagga ctttttgtat ttcctttttt tttcagtatt tctgcatatt
     2941 ccctgcagtc tgaatataca gtaatatcgg ctttccagtc aggatagtca ttccgcagtt
     3001 cagctgcaaa attgtacatc acattcagat aggattcgga atattccaga aacagatctg
     3061 aaaactgctt cgg
//
LOCUS       DAOQGJ010000240         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_18915_length_3071_cov_3.751326, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000240 DAOQGJ010000000
VERSION     DAOQGJ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18915_length_3071_cov_3.751326"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK683_05025"
     CDS             complement(<1..566)
                     /locus_tag="PK683_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNH07842.1"
                     /translation="MGEGSLSQDEIDALLQGADESSFAPDPVQPSGPADPFSPTDKDF
                     LTEIISSAFGVAANTLESVLAKPVRFTSPIVEAKTAQDLAAELGAESVSLVSNLTGAV
                     NGRTSLVISVENASKIVSVLMGGLSTGGFDSARQQTLKNNFAPVMGMVGVQIGLKLNS
                     VVQASPLEISMAKTPKDMVLPDGKMLVK"
     gene            818..1474
                     /locus_tag="PK683_05030"
     CDS             818..1474
                     /locus_tag="PK683_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07843.1"
                     /translation="MISPEKSVSFECNYPILVEIYESIKNINDLSFKIRLRALNILVS
                     VSRDIGTVTGFKAISFELIRYSNLMESESSKLRELIHKILVAHTFQKKIDRTVRFIRR
                     IEIHTNAETQRFYRSDLDSIFLENERKHLSLNENIYESIQLLMKFTKEFFKICMKGNS
                     IAVLAKIESAYMKEQREVYMKLAEDVERFLAETMASLKNILTSLGIDISNEENKIYLH
                     "
     gene            1446..2192
                     /locus_tag="PK683_05035"
     CDS             1446..2192
                     /locus_tag="PK683_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH07844.1"
                     /translation="MKKIKYIYTDKDHKWISVYRDKEKQSYIIDTNEYIVMNGSDSLI
                     TDPGGVEIFPTVFTSISSEVNPMAIRYIFSSHQDPDVISSLSLWLEVNPMIKCYVSWL
                     WSTFLPHFGGNEKVFISLPDEGMKISLGTLDLEAVPAHYLHSSGNFHLYDPKAKILFS
                     GDIGAALLPESDDSDLYVKDFDRHIEYIEGFHRRWMGSDEAKMDWCERISRYEIDMLC
                     PQHGLIYTGKDVERFINWFSELKVGITRKS"
     gene            2292..>3071
                     /locus_tag="PK683_05040"
     CDS             2292..>3071
                     /locus_tag="PK683_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07845.1"
                     /translation="MRIIFFIIFFINLSLSAEENLTELKGWKFFFSDLKTDAQDAEKA
                     SWKEPENGRPYMKNQAGSVLWLKAYLPEHKQQDPTVYVRIIVQNVEVYQDRKLIYRFG
                     EIGKDLFGSPVHFINIPENSRSPLYFRISSKEENVGLRYEVMYGSGRQIVQWIIKKDA
                     ERFGFGFLYIFIGLFASLLYIRRKLRIYLSFGLLSVCIGIFFISSRATGIQHLLYPAP
                     FFWLYAGGISMYLIPVYLSWFTRHISNYTKNIIMYISYAHLA"
BASE COUNT          930 a          635 c          636 g          870 t
ORIGIN      
        1 atcttgacga gcatcttccc gtctggaaga accatatctt taggtgtttt tgccatggag
       61 atttccagag gcgaagcctg aaccactgaa ttcagcttca gtccgatctg cactccaacc
      121 attcccataa ctggggcaaa attgtttttg agagtctgct gtctggcact gtcaaatcct
      181 cctgtcgaga gtcctcccat aaggacactt acgattttag aggcgttttc aacggaaatg
      241 accaaacttg tccttccatt gaccgctcct gtcaggttgg aaaccaagga taccgattct
      301 gctcccagtt cagcggccaa atcctgtgca gttttggctt ctacaattgg actggtaaat
      361 ctgaccggct tagctagaac actctccagt gtatttgctg ccacaccaaa tgcagaggaa
      421 ataatctcag tcagaaaatc cttgtctgtg ggagaaaatg gatcagcagg gccagatggc
      481 tgaacaggat caggcgcgaa agaactttca tcagctccct gtaaaagtgc gtcgatttcg
      541 tcttgtgaaa gtgaaccttc tcccatgtat tactccagat aaaatttgtc gtactgctat
      601 tctaattcag cagcgcatac tgtaaatatt ttcggaaaga aaaggggaaa aattttcaga
      661 aaaacgagaa attcataaac ttttccatta aatcaaacga actgaagaaa aacccttgtt
      721 tcctgtcggt tttggcaggc ttcctgtcta tttcccaggt gcaaaatgaa aaatgcttga
      781 ctgcatccgg aaaactcaaa tttttcatat agaccaaatg atttcccccg aaaaatcagt
      841 cagttttgaa tgcaattatc ccattctggt ggaaatttat gaatccataa agaatatcaa
      901 cgatctttca ttcaaaatcc gtcttagggc actgaacatc cttgtttctg taagcaggga
      961 catcggaaca gtgaccggtt ttaaggcaat ttcgtttgaa ctgatcagat acagtaatct
     1021 gatggagtct gagtcatcca aactgagaga actgattcat aaaatcctgg tcgctcacac
     1081 ttttcagaaa aaaattgaca gaacagtgcg gtttatacgc agaatagaaa tccatacaaa
     1141 tgcagaaact cagagatttt acaggtctga tctggacagc attttcctgg aaaatgaaag
     1201 aaaacatttg agcctgaatg aaaacatata tgaatcgatt cagcttctga tgaaatttac
     1261 caaggaattt tttaaaatat gcatgaaggg caactcaatt gcagtcctgg caaaaattga
     1321 atcggcatat atgaaggaac agagagaggt ctatatgaaa ctcgccgaag atgtggaacg
     1381 gttccttgct gaaacaatgg catcattaaa aaatatcctc actagcctgg gaatagacat
     1441 aagcaatgaa gaaaataaaa tatatttaca ctgacaagga tcacaagtgg atctctgtct
     1501 acagagacaa ggaaaaacag tcctatatta tagatacaaa tgaatatatt gtaatgaacg
     1561 gctcagattc actgatcact gatccgggcg gtgtggaaat tttcccgact gtattcactt
     1621 ccataagctc tgaagtaaat ccaatggcaa tacgttatat attcagttcg catcaggatc
     1681 cggatgtcat ttcttcctta agtctctggc tggaagttaa cccgatgatc aaatgttatg
     1741 tcagctggct ctggtcgact ttcctgcctc atttcggcgg gaatgaaaaa gtattcatat
     1801 cacttcctga tgagggaatg aaaatcagtc tgggcactct cgacttggaa gctgttcctg
     1861 cacactatct tcattcctcc ggaaattttc atctgtatga tccaaaggca aaaattcttt
     1921 tttccggaga cattggagcc gcccttcttc ctgagtcgga tgactcagat ctgtatgtaa
     1981 aggattttga cagacatatt gaatacattg aaggtttcca cagaagatgg atgggatcag
     2041 acgaagcaaa gatggactgg tgcgaaagaa tcagcagata tgaaattgat atgctctgcc
     2101 ctcagcatgg tctgatctat accggcaagg atgtagaacg cttcatcaac tggttctctg
     2161 aactgaaagt gggaataacc cgaaagtcct gaagcatatc ccgtctgccc tgcaccggag
     2221 cggacagaga ataaagattt ttgaataatg aaaagacaat atcggatttc accggctcag
     2281 ggtttctgct tttgagaata atatttttta taattttttt tataaattta tctctttctg
     2341 ccgaagaaaa tcttactgag ctgaagggat ggaagttttt cttttccgac ctgaagacgg
     2401 atgcacagga tgcagagaaa gcttcctgga aggaaccgga aaacggccgt ccttatatga
     2461 aaaatcaggc tggatcagtc ctttggctga aagcatatct tccagagcat aaacagcagg
     2521 accccacagt ctatgtaaga atcattgttc agaatgtgga agtgtaccag gacagaaagc
     2581 tgatttaccg ctttggggaa atcggcaagg atcttttcgg aagcccggtc catttcataa
     2641 acatccctga aaacagcaga agccctctgt acttcagaat atcctcaaaa gaagaaaatg
     2701 tcggtcttcg gtatgaagtg atgtacggtt cgggaagaca gattgttcag tggatcatta
     2761 aaaaagatgc tgagaggttc gggttcggat tcctgtatat tttcataggt ctttttgcaa
     2821 gtctgctcta tatccgcaga aaactcagaa tttatctttc cttcggtctg ctttccgtct
     2881 gcatcggcat attttttatc tccagcagag ctacaggcat tcagcatctt ctttatcctg
     2941 ctccgttttt ctggctctat gcaggcggaa tcagtatgta tcttattccc gtatacctga
     3001 gctggtttac ccgacatatc tccaattata caaaaaatat tataatgtat atatcctatg
     3061 ctcatctggc a
//
LOCUS       DAOQGJ010000241         3059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19047_length_3059_cov_3.189414, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000241 DAOQGJ010000000
VERSION     DAOQGJ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19047_length_3059_cov_3.189414"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2842
                     /locus_tag="PK683_05045"
     CDS             <1..2842
                     /locus_tag="PK683_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07846.1"
                     /translation="DIYSARYKPGKSKPLSDKNMLAETEGLKPFFLLLKTEEHFTQWA
                     LFLKNYGNRKDSFELFVWTEVFNVPLLKILPAMEILNSSAFERLQSFYFKDPAVLRFL
                     IRSIMTSLHELKMRNLNPSSYKFILKLFLNLKIDSEFLRRRAFFFHLLREYSSSRYME
                     LEMIRYIFFEYIFIATKTVTDYKYGMTRFCDDSHRVFCEWVDGAYMEALRLDPKERVG
                     FFKPVFDYFRAVIWTEHTQKFGKYKYRFEQFQSAVISANLEDAAVDELKKVETEEFPA
                     YHESFFNVFYTKPERMNEAVSSDYWYRLSLDQARPHIQSILLNLIGTINLMPGQSGAY
                     TDGSSVYLPEYQNSFKDPHDPIEDNRNLTLYIGFALHECGHIIAGTFRFNLEYYLRTL
                     EKPFLFKMIMNIFEDHRIEAFLIRMKIHAQSKEILKNLNEFLSVRNWKNEMPVFMRFL
                     LFCFDRANGYEEILKNFSDYEEKLKSILSLTVNTGRFSSLKSLMDYISDRLKGMEIGN
                     PMAAYELSREVYEILKHWPMQEEDEEEQRPAKGMHRMAGGEEGSDAPAPLTVLNEEEL
                     RELYRQYNDNPRQFVQDYNLPYIPEIHSGGKNGKESAEASPSSEWITEEEPITNYDES
                     GTIDFSHRTKADDLSAEDRLIQTEERKGKSVKKRKKKTGAKKVYSIDPNTKSRTRLSE
                     IKEHMVTKTDMKFLKKIRRWDFVRHRIMEEISRLELRSKEDHDISAVEGEINMELLLE
                     IISGKDKVGVFEFLDIYTETKKSLDVIIGIDASGSTASDASEGYTVIEVEKAFAVIFG
                     EALSAFTENVKCLAFDSLTSTTVYRAATLKNISSVQPGNGNRDGDFIRFVSDELKTGT
                     SELKYFFFLSDGQPNSDNYSGKEAMDDTLIALREARKSGIRIIYFNIDSEKREYFDQF
                     AKETTFAQHFKNPTDLIPVIPELIRSMAKAVG"
BASE COUNT         1003 a          619 c          658 g          779 t
ORIGIN      
        1 ggatatatat tcagcaagat acaaaccggg caaatccaaa cctctttcag acaaaaatat
       61 gcttgcagaa acagaaggac tgaagccttt ttttctgctt ctgaaaacag aggaacattt
      121 cactcaatgg gcgctttttc tgaaaaacta tggaaacaga aaggacagtt ttgaactgtt
      181 cgtatggact gaggtattca atgttcctct tctgaaaatt cttcctgcaa tggaaatact
      241 gaattccagc gcatttgaaa gacttcagag tttttacttc aaagaccctg cagttctccg
      301 cttcctgatt cggagcatta tgacatctct gcatgaactg aaaatgagaa atctgaaccc
      361 atcctcctat aaattcattc tgaaactttt cctgaacctg aaaatcgatt ccgaatttct
      421 gagaagaagg gcttttttct ttcatcttct acgagaatac tcttcctcca gatatatgga
      481 actggaaatg atacggtata tattttttga atatattttc attgcgacaa agactgtgac
      541 agactataag tacggaatga ccagattctg tgatgacagt caccgggtct tctgtgaatg
      601 ggtcgatgga gcctatatgg aagcactgag acttgatccg aaagaaagag taggattttt
      661 caagccggta ttcgactatt tccgtgcagt catctggaca gaacatactc agaaattcgg
      721 caaatacaaa tacagatttg aacagtttca gtctgcggtc atatctgcaa acctggaaga
      781 tgctgcagtg gatgaactga aaaaggtgga gacggaagag tttcctgcct accatgaaag
      841 tttttttaat gttttttata ccaaaccgga aagaatgaat gaggcagttt cctcagacta
      901 ctggtacagg ctttcactcg atcaggccag acctcatata cagagtattc ttctgaatct
      961 gatcggcaca ataaatctga tgcccgggca gtcaggagca tacactgacg gttcatctgt
     1021 ataccttccg gaataccaga actccttcaa agatcctcat gatccgatag aagacaacag
     1081 aaatcttaca ctgtacattg gattcgctct gcatgaatgc ggtcatatca ttgcaggaac
     1141 attccggttc aatctggaat actatctcag aactctggaa aagccttttc tgttcaagat
     1201 gattatgaac atatttgagg atcacagaat agaggcattc ctgatcagga tgaaaatcca
     1261 tgctcagtcc aaagagatac tgaagaatct gaatgagttt ctgtctgtca gaaactggaa
     1321 aaatgaaatg ccggtattca tgagattcct tcttttctgt ttcgaccgtg caaacggata
     1381 tgaagagatt ctgaaaaatt tttcagacta tgaggaaaag cttaaaagca ttctttctct
     1441 gacagtcaat acaggccgat tcagcagtct gaagagcctg atggattaca tttccgaccg
     1501 actgaaaggg atggagatcg gaaatcctat ggctgcttat gaactcagca gagaagtata
     1561 tgaaatactc aaacactggc ctatgcagga agaggatgaa gaagaacaga gacctgcaaa
     1621 aggaatgcac aggatggctg gcggagagga aggatcagat gctcctgctc ctctgacagt
     1681 tctgaatgag gaagaactca gagaactgta tagacagtat aatgataatc ccaggcagtt
     1741 tgtacaggat tataatctgc cgtatattcc tgaaatacat tccggcggaa agaacgggaa
     1801 ggaatccgca gaagcttctc cctcttcgga atggatcact gaagaagagc cgatcacaaa
     1861 ttacgatgaa tcaggaacaa ttgatttcag ccacagaacg aaggcggacg atctttctgc
     1921 agaagacaga ctgattcaga cagaggaaag aaaaggaaaa tctgtcaaaa aaagaaaaaa
     1981 gaaaactggc gcaaagaaag tctattccat tgatccgaat acaaaatcca gaacccgact
     2041 gtccgaaatc aaggaacata tggtgactaa gacagatatg aagtttctga aaaaaatcag
     2101 aagatgggat tttgtgcgcc atagaattat ggaagaaatt tccagactgg aactccgctc
     2161 aaaggaagat catgatattt ctgcagtgga aggagaaatc aatatggagc ttcttctcga
     2221 aatcatcagc ggaaaagaca aggtgggagt tttcgaattt ctcgacatct atacggagac
     2281 caaaaagagc ctggacgtca tcataggaat agatgcaagc ggctcaactg catcagatgc
     2341 aagcgaagga tacactgtga tagaagtgga aaaagcattc gcagtcatat tcggagaagc
     2401 actttctgca ttcactgaaa atgtaaaatg tcttgcgttt gattcactca catccactac
     2461 agtgtacagg gcagccacgc tcaagaatat cagttcagtc cagccgggaa acggaaacag
     2521 agacggcgat ttcatccgat ttgtctccga tgaactgaag acagggacat ccgaactgaa
     2581 atacttcttc ttcctctccg acggacagcc caactcggac aattacagcg gaaaggaagc
     2641 aatggacgac acactgattg cactgcgtga agccagaaag tctgggatac gtattatcta
     2701 ttttaatata gacagtgaaa aaagagaata cttcgatcag ttcgcaaagg aaaccacatt
     2761 cgcacagcat ttcaaaaatc cgactgacct gatccctgtc attcctgaac tgatccggtc
     2821 aatggcaaag gcagttggat agaaatactt ggacaatatt cgactgtcca tacatatccc
     2881 ctgaaaggac tcccttccct aggcttttga cagatgaatg aagtctattc cttatcaatt
     2941 ctcttcttcc atccgtcatg attgcggcta ttatgccaga cggcgattac agatatgaac
     3001 gcttctttta tgagataaaa aatatagaag ggaaattcag gaacgggagc ttttcttac
//
LOCUS       DAOQGJ010000242         3053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19104_length_3053_cov_2.988659, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000242 DAOQGJ010000000
VERSION     DAOQGJ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19104_length_3053_cov_2.988659"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PK683_05050"
     CDS             <1..689
                     /locus_tag="PK683_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07847.1"
                     /translation="QEKYPFDGGLGFFSVSFGAGLGLIALSEPSIQKRFSSICVVGGF
                     ADFEAVVPHVLEHFETENYGNFILLHNFIHLTVKNSQKLESVLWEAAMDNGLNRKNGE
                     AVAPKLQSELDLRDKQIFERICKEKSYRTALGEEIRETAGLLLRDLSPIHYIKDISIP
                     VSIIHGRNDYVIPETESIRISEQMNKCGMQNRLEITSLLSHGDKVSYWKYLSEIPGLA
                     EAFGYFLIRI"
     gene            754..2391
                     /locus_tag="PK683_05055"
     CDS             754..2391
                     /locus_tag="PK683_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNH07848.1"
                     /translation="MPAIPSVTSMMNALPKTDSMPAIQESGQDQEKKAEETSAESSVK
                     KEPKAPVHLPKKGDFASRLRIEILPGDMQAFLLLDTTGMDFTDLNPTSVYAYLNTTKL
                     DREITSYNKINDLIDQCKKIQAGQVSNPIIQVLICQGTPPVQGIDGWIKYYHPHNQKV
                     VIHENDKADFRNLDRYITIKQGDKIATMFMGVPGIPGKDVYGNITNPTPIKKPKINIG
                     KNILTETITNPEEPEKVYMEYSAGCNGVLISNDETVTVSEELQINTNVGLSTGNISYE
                     GSITVRGSIEEGAKVTCTGSLLVHENIESTDIEVGGDLIVKSGIKVKGKSIIRVKGNI
                     KTKFIENAVIETDGDVIVDSFILNSKIFSLGSVILTGQNGSILGSEIVVYGGLSTTNL
                     GSAAGVDILVEMGYHFRNEKLFHDLTSALKVSEKELTVLVPQIQQMNSIIKQSRGKID
                     EAKKLKYKQMIEVYQKKTEVHKKLLLKYDELKNTRFNTEKINLVVKGGGFPGVTVKYR
                     RQIEKITVPQSSFMMTFFPGQDQAPMTAISGSASKKK"
     gene            2407..>3053
                     /locus_tag="PK683_05060"
     CDS             2407..>3053
                     /locus_tag="PK683_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH07849.1"
                     /translation="MTSVKRKIIVIGASSGIGAEIAKQLLCNNNSVTFIARRRSEIEK
                     RIAECGELEQFSKPFIIEHDVKNITEVPALFEKAVADMGGLDEIYFAAGIMHKVSIDE
                     FNTAKDIEMMQVNTLGAIAWINLAAAYFSSKGRGKIIGISSIAGDRGRVGNPVYNTSK
                     AAMNTYLEALRNRLARKGILVLTVKPGFIDTDMTKGMQGLFWLISAAKAAEIIIRA"
BASE COUNT          989 a          574 c          707 g          783 t
ORIGIN      
        1 ttcaggaaaa gtaccccttt gacgggggac tgggcttttt ttctgttagt ttcggtgcag
       61 gactgggtct gattgctctc tctgagcctt caattcagaa aagattttct tctatctgtg
      121 tagtcggcgg gttcgcagac tttgaagcag tagttcctca tgtcctggaa cacttcgaaa
      181 ccgaaaatta tggaaatttt atacttcttc acaattttat ccatcttacg gtaaagaatt
      241 cccaaaaatt ggagtcagta ctatgggaag ccgcaatgga taatgggtta aacaggaaaa
      301 acggtgaagc agtggctcca aagctgcaat ctgaactcga tttgagagat aaacaaattt
      361 ttgaaagaat ttgtaaagaa aaatcttaca gaactgcatt gggagaagaa attcgtgaaa
      421 ctgcgggttt acttctaagg gatttatctc cgatacatta tattaaggac atttcgattc
      481 cggtcagcat tattcatgga agaaatgact atgtgattcc tgaaacagaa tccatacgaa
      541 tttcggagca gatgaacaaa tgcgggatgc agaaccggtt ggaaattacc agtcttctgt
      601 ctcatggtga caaggtttcc tattggaaat acttaagtga gattccggga ctggcggaag
      661 cctttggcta ttttttgata cggatatagg aagaataaaa tgactgagca gaataaagat
      721 caacagcctg aaaatccggg cactaacttt aagatgccgg ccattccgtc tgtaacgtca
      781 atgatgaatg cactcccgaa aacagattca atgccggcaa ttcaggaatc aggtcaggat
      841 caggaaaaga aagcggaaga aacatccgca gagtcttctg taaaaaaaga acccaaggca
      901 cctgttcacc tccctaaaaa aggcgatttt gcctcgagac tcagaataga aattcttccg
      961 ggggatatgc aagcatttct tcttctggat acgacaggaa tggattttac tgatctgaat
     1021 cctacgtctg tctatgctta tctgaatacc actaaactgg acagggagat cacttcctac
     1081 aataagatca atgacctcat tgatcagtgc aagaaaatac aggccggtca ggtcagcaat
     1141 ccaattattc aggtactgat ctgtcaggga acacctccag tccaagggat tgacggctgg
     1201 ataaaatact accaccctca caatcagaaa gtggtcattc acgagaatga caaggccgat
     1261 tttaggaact tggacaggta cattaccatt aagcagggtg ataagattgc cactatgttt
     1321 atgggagttc ctgggattcc cggtaaagat gtctacggaa atatcaccaa tccgactccg
     1381 attaaaaagc ccaaaatcaa tatcggaaag aatattttaa cggaaacgat aaccaacccc
     1441 gaagaacctg aaaaagtgta catggagtat tcggcaggat gcaacggggt acttatttca
     1501 aatgatgaaa cggttactgt atccgaagaa cttcagatta atacaaatgt tggactttcg
     1561 acaggaaata tttcctacga aggatcaatt acggtacgcg ggtccattga agagggagcc
     1621 aaggttactt gtacaggaag cctgcttgtt catgagaata tagaaagtac tgatattgaa
     1681 gtaggcgggg atctgatcgt taaaagcggg atcaaagtca aaggaaagtc gattatccgt
     1741 gtcaagggaa acatcaagac taaattcatt gaaaatgcgg tcattgaaac ggacggagat
     1801 gtgattgtag acagcttcat tctgaactca aaaattttca gtctgggaag cgtcatattg
     1861 acagggcaga acggatctat tctcggttcg gaaatagttg tctatggcgg cctttccact
     1921 accaatctag gctctgcggc tggtgtagat atacttgtgg aaatgggata tcattttcgg
     1981 aatgaaaaac tgtttcatga tcttacaagt gcacttaagg tttcggagaa agaactgacg
     2041 gttcttgttc ctcagattca gcagatgaat tccatcatca agcagtccag aggaaaaatt
     2101 gacgaggcca agaaactgaa atacaagcag atgattgaag tctatcagaa gaagacagaa
     2161 gtgcataaaa agcttcttct gaagtatgat gaactgaaaa atacacgctt taatacagag
     2221 aaaatcaatc tggtcgtcaa agggggaggt ttccccggtg tgactgtcaa gtacagaaga
     2281 cagattgaaa agattacagt tcctcagtcc agctttatga tgactttttt tccaggacag
     2341 gatcaggctc cgatgactgc catttccgga agtgcgtcca agaaaaaata agacaggaaa
     2401 aaatatatga catcagtcaa aagaaaaatc atcgttatcg gtgcatcttc aggaatcgga
     2461 gcagaaattg ccaaacagct tctctgcaat aataattccg tcaccttcat cgcaagaaga
     2521 agatccgaaa ttgaaaaaag aatagcggaa tgcggtgaac tggaacagtt ttcaaaacct
     2581 tttattattg aacatgacgt aaagaatata acagaagtgc ccgctctttt tgagaaggca
     2641 gtcgcggata tgggcggact cgatgagatt tattttgctg caggaattat gcataaggtt
     2701 tccatcgatg aattcaatac tgcaaaagac attgagatga tgcaggtgaa taccctcggc
     2761 gcaatcgcct ggatcaatct tgccgctgct tatttttctt caaaaggaag aggaaagatc
     2821 atcggtattt cttccatagc aggggatcgg ggcagggtag gaaatccagt atataatacc
     2881 agcaaggcgg caatgaatac ttatctggaa gcattgagaa acagactggc cagaaaggga
     2941 attctagtac tgacagtgaa gcctgggttt atcgacacgg atatgactaa gggaatgcag
     3001 ggacttttct ggctgatttc tgccgcgaag gctgctgaaa tcattatccg tgc
//
LOCUS       DAOQGJ010000243         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19127_length_3051_cov_3.828772, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000243 DAOQGJ010000000
VERSION     DAOQGJ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19127_length_3051_cov_3.828772"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PK683_05065"
     CDS             complement(<1..981)
                     /locus_tag="PK683_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HNH07850.1"
                     /translation="MDQAKEVIDQIIEICDSEGKRFYDFFFPPSKRSYSHYPPSYLKD
                     DKKEFGTDYTWKRFSDIHIDPGVFIKKIQPGDIDQGTIGNCYMIAEISSFAQRPDLVK
                     EAIYPNEWNSAGIFGVKIWIETMQEWRMVILDDFFPVDMNDYFFFACSKTKNEAWSSL
                     LEKAIAKINGCYLSLEFGNKVEVVPALTGQSIALLLSESPEEVFEAIRYYSSNGGLVQ
                     CSGGTILKDGTYTVAKREEGLVQGHAYSVLDAVEMDGFRLIKLRNAWGHFEWNGDWSD
                     DSDLWNQYPEISEACKHKISENGIFWMSLEDFSGKMGTIKVTAIGFDHLPD"
     gene            complement(1527..1934)
                     /locus_tag="PK683_05070"
     CDS             complement(1527..1934)
                     /locus_tag="PK683_05070"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNH07851.1"
                     /translation="MKNKMNKEVTAFLEELNHPLIQEISLLRKIILETDAELQENIKW
                     NGPNYLHNNDDRITMRIHPPKQIQLIFHRGAKVKQQPKKRLISDETGLLVWKENDRAV
                     VSFKNEDELKKNKKNIQKWIQDWTLAGVESNIA"
     gene            complement(1983..>3051)
                     /locus_tag="PK683_05075"
     CDS             complement(1983..>3051)
                     /locus_tag="PK683_05075"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07852.1"
                     /translation="AREMRDGLRNIYSVMKGADAYIKFAFITGVSKFSKINLFSGLNN
                     LQDITLNKRYAAVCGYTENELSVFQERLEDVNRDKLKLWYNGYNFLGDSVYNPFDILL
                     FLDSKEYRNYWFETGNPSFLIDLIREKNYNSINMNKVRLTDSALSSFDVDSIELENLL
                     FQTGYLTIKSIKEMQANRVYYLQYPNLEVRSSLTDVILTYLAKDTQPKEKNKVDLYEA
                     LQENDLLKLKNIFYSFFASIPHDWYRKNTLANYEGYYASIFYCYFTAIGLDVRAEDVT
                     NQGQVDMTVFFEDRVYVFEFKVIEMTEAGSALQQIKDKRYYEKYLSTIAGDGRDRSLH
                     LIGVEFSRKSRNITNYEWEKL"
BASE COUNT          871 a          662 c          513 g         1005 t
ORIGIN      
        1 gtcgggaagg tggtcaaatc cgatggctgt aactttaatt gtccccattt ttccagagaa
       61 atcctcaaga ctcatccaaa aaataccatt ttcagatatc ttgtgcttac aagcctctga
      121 tatttcggga tactggttcc ataaatcgga atcatcactc cagtccccat tccattcaaa
      181 atgaccccat gcatttctga gcttgatcag tcgaaatcca tccatttcca ccgcatccaa
      241 tacagaataa gcatgaccct gaacaagtcc ttcttctctt ttcgctactg tataagttcc
      301 atctttcaga attgttcctc cgctgcactg gacgagacct ccattcgaag aatagtaccg
      361 gattgcctca aatacttcct ccggggattc agacagcagt aaagcaatag actgacctgt
      421 caaagccgga actacttcta ctttattacc aaattccaaa gataaatagc atccattgat
      481 tttggcaatt gctttttcta agagagatga ccaggcttca ttttttgtct tactgcaggc
      541 aaagaaaaaa tagtcattca tatcaactgg gaaaaaatca tctaatatta ccatacgcca
      601 ttcctgcatt gtctcaatcc aaatcttaac accgaatatg ccggcagagt tccattcatt
      661 cggataaatg gcttccttta ccagatcagg gcgctgagca aaggatgaga tttcagcaat
      721 catataacag tttccaatag taccctgatc aatgtctcct ggctgaattt tttttataaa
      781 tacacccgga tcaatatgaa tatctgaaaa tcgtttccaa gtataatctg taccaaactc
      841 cttcttatcg tctttcaaat aggaaggagg atagtgactg tatgaccgtt tacttggtgg
      901 gaaaaaaaaa tcgtaaaaac gcttaccttc tgaatcgcat atttcaataa tctgatcaat
      961 cacctctttt gcctgatcca aaggaaacag aactctctgt ttgacctgag ccgcttcttc
     1021 ataaagaggt tctaaaagat cccttgcatc ataatcattc tcacccatat taattactgt
     1081 attattcttt gattttttca taccatgcat ataatattga aaaataaaaa gtcaatgctt
     1141 ttttaatcaa agagaaaatt tgaagggtgc agtaaatgag gaatctgaag ctgatctttc
     1201 caaacgagac ttatactctg aatgaatatc aaaactgttt ccaaagatct gttcattttt
     1261 tgaaatataa caactcacct tacgcttccg gctgaactgt cggcggcttt cggactcaca
     1321 aggcagtacc ctgctgtttt tttactgatt catcgcaagc tgttaaaatg ctggattcct
     1381 ataatgcttc atttagaata cattcttcat acattgcatc cttttataga atgtgagata
     1441 atatattaca atgtattgat atactgctat tttaatgcag tcaatgatca attgacttta
     1501 ctcaaatttc attagaaaat cgaatattat gctatgttcg attcaacacc ggccagggtc
     1561 cagtcctgta tccatttttg tatgtttttt ttattttttt ttaattcatc ttcattttta
     1621 aaggatacga ccgccctgtc attttccttc caaaccagaa gacctgtttc atccgagatc
     1681 agtctttttt tcggctgctg ttttactttt gcacctctgt gaaatatcag ctggatctgc
     1741 ttaggcggat ggattctcat cgtaattctg tcatcattat tgtgcagata gttcggaccg
     1801 ttccatttga tgttttcctg cagttctgca tccgtttcaa gaatgatctt tcggagcaga
     1861 ctgatttcct gaatcagagg atgattcaat tcttccagga aagcggtcac ttctttattc
     1921 attttattct tcattgtctg aatgcctgcc tttcttttat ccctgcactg agatgtttcc
     1981 gatcagagtt tttcccattc ataatttgta atattcctgc tctttctgga aaattccacg
     2041 ccgatcagat gtaaggaacg gtcacgaccg tcccctgcga tggtcgataa atatttttca
     2101 taataacgct tatctttgat ctgctgcagt gctgaccccg cctcagtcat ctcaatcacc
     2161 ttgaattcaa atacataaac cctgtcttca aagaatacag tcatatccac ctgtccctga
     2221 ttcgttacgt cctctgcacg cacatccaga ccgatggcag taaagtaaca atagaatata
     2281 gatgcatagt atccctcata gtttgccaga gtattctttc tgtaccagtc gtgagggatg
     2341 gaggcaaaga aggaataaaa aatattcttc agtttcagaa gatcattttc ctgaagagcc
     2401 tcatagagat ccactttgtt cttctctttg ggctgagtgt cctttgccag ataagtcagt
     2461 attacatctg tcagactgct tctgacttcc agattgggat actgaagata atagactcga
     2521 tttgcctgca tttccttgat gctttttatt gtgaggtatc cggtctgaaa cagaagattc
     2581 tccagttcga tagaatccac atcaaaactc gaaagggcgg aatcagtcag tctgaccttg
     2641 ttcatattga tggaattata atttttttcg cggatcagat caatcagaaa ggaaggattt
     2701 ccagtttcaa accaatagtt tctgtattct ttgctgtcga ggaaaagcag aatgtcaaat
     2761 gggttgtata cactgtctcc cagaaaattg tatccattat accataattt taatttatcc
     2821 ctgtttacat cttcgagtct ttcttgaaaa acggaaagct cattttcagt gtacccgcat
     2881 acagcggcat aacgtttgtt cagtgtaatg tcctgaagat tattcaatcc gctaaacaga
     2941 ttgatcttgg aaaatttaga tacgcctgtg ataaatgcaa atttaatgta tgcatccgct
     3001 cctttcatta cggaataaat attgcgcaga ccgtccctca tttctcttgc a
//
LOCUS       DAOQGJ010000244         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19172_length_3047_cov_2.635695, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000244 DAOQGJ010000000
VERSION     DAOQGJ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19172_length_3047_cov_2.635695"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..145
                     /locus_tag="PK683_05080"
     CDS             2..145
                     /locus_tag="PK683_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH07853.1"
                     /translation="MLDEPERTVLSVAYAVGFNSKSAFSKAFRTFTGITAQEYRKKYS
                     VRK"
     gene            261..2141
                     /locus_tag="PK683_05085"
     CDS             261..2141
                     /locus_tag="PK683_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNH07854.1"
                     /translation="MYSNLAEMFVISSEKYSDFPAFASRDASGKFHSIKYGELKENAE
                     CLSAALLDLGAEPGDRIGIFSDNRLEWILADMAVIMAGCADVPRGSDVTDGDIKYIIS
                     HSEAEILFVENEEVLKKIRRNSADLPHLRTLIMMDRNSKATDSILHIYDLMEKGKRIL
                     SKNPSCVRDRIRSISGDDLFTLIYTSGTTGTPKGVMLTHRNMVSQLKNNPIEIHSSDR
                     ILSILPVWHIFERVFEMTALSKGCCTYYTNVRNFREDLKTVRPTFMASAPRLWESVYQ
                     GIMSNLERASIIRKKMFSTAYYLASAVRSAQRFLSGQAPDLEGRTLMLSVLRGAESII
                     TAALLYIPFRIMDSLVLSKIREATGGQLRGSCSGGGALPLHVDEFFCNVGIPVLEGYG
                     MTETSPVISVRTFQNLVPGTVGPLYKGTEIRLLDIHTGEQIYPGKNGFGRKGEIHVRG
                     EQVMKGYFKNQEATEKVLKDGWLNTGDLGMMTYNGCLKITGRSKETIVLLGGENVEPV
                     PLENRLLESPLIDQCMVAGQDRKFLSALIVPRMERFREYGSSLSELSENESVRKILRE
                     ELKRLVSTENGFKSFEKISDFRILPKSFEAGDELSAKLSIKRHIITEKYSHLIDDMYK
                     KA"
     gene            complement(2186..2986)
                     /locus_tag="PK683_05090"
     CDS             complement(2186..2986)
                     /locus_tag="PK683_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNH07855.1"
                     /translation="MKKIIPYMTAAVLLFHCSALPEKYTSPTGRYRIITVDDPEMNQM
                     GAKAFEDLKKKTPAETDPNTNAYVKCIANAITGVISDSTGVQSWEVVVFNDSTANAFA
                     LPGGKIGVHTGILPVAKTADQLAAVMGHEVAHVLARHGAERISEGGLLQKGMEAIGSA
                     TKNDPKIMGAIGAGAQFGVMLPFSRKHETEADILGLEYMAKAGFDPSESINLWKNMNA
                     AGGSKPPEIMSTHPSDQTRMNNLNEKLPDAKKLYDEAKAKGKNPNCRL"
BASE COUNT          831 a          616 c          705 g          895 t
ORIGIN      
        1 gattctcgat gagccggaaa gaacagtgct ttcagtggca tacgctgtag gttttaactc
       61 aaaatcagct ttcagcaagg cgttccgtac attcaccgga atcactgctc aggaataccg
      121 aaaaaaatat tctgtcagaa aatgattttg ggagttcttt tctataacct gattccattt
      181 ttccggttgc tttctataac tcaggtcgat ctgaattcct ttttttccta taatcagtta
      241 aaagctgaag gagaaaacag atgtattcaa atcttgcaga gatgtttgtc atctcatctg
      301 aaaaatacag tgattttccc gcatttgcat caagagatgc ttcggggaaa tttcattcaa
      361 taaaatacgg tgaactgaag gagaacgcag aatgcctttc tgcggccttg ctcgatctgg
      421 gagcagagcc tggagaccgt atcggcattt tctcggacaa caggctggaa tggattcttg
      481 ccgatatggc agtgattatg gctggctgtg cagatgttcc gaggggaagt gatgttacag
      541 acggggatat aaaatatatc atttcccatt ctgaagcaga aatactgttt gtggaaaatg
      601 aagaggtgct taagaaaatc agaagaaatt ctgcggatct tccgcatctg agaacgctga
      661 ttatgatgga cagaaattcg aaagcaaccg acagcattct tcacatttat gatctgatgg
      721 aaaaaggaaa aagaattctc tctaaaaatc catcctgtgt tcgggacaga ataagaagca
      781 tatcgggtga tgatctgttc actctgatat acacttcagg cactacagga actccgaaag
      841 gagtcatgct gactcacaga aatatggttt cacagctgaa aaacaatccg atagagattc
      901 attcttctga ccgaattctc tccattcttc cagtctggca tatctttgaa agagtgtttg
      961 aaatgactgc actctcaaaa ggctgctgca cgtactatac caatgtaagg aatttcaggg
     1021 aggaccttaa gactgtgaga ccgaccttta tggcctctgc tcccagactt tgggaaagcg
     1081 tataccaggg aataatgtca aatctggaga gggcatccat tataagaaaa aagatgttca
     1141 gtacagccta ttatcttgct tctgcagtcc gttcagcaca gcggttcctt tccggacagg
     1201 ctcctgatct tgagggaaga acactgatgc tttctgtttt gagaggagca gaaagtataa
     1261 ttacggctgc actgctttat attccgttca ggatcatgga cagtcttgtt ctttctaaaa
     1321 tcagagaagc gacaggagga cagctgagag gttcctgctc cggaggaggg gctcttcctc
     1381 tgcatgtgga tgaattcttc tgcaatgtcg gcatcccagt ccttgaaggg tacggaatga
     1441 cagaaaccag tccggtgata tccgttcgga catttcagaa tcttgtcccg ggaacggtag
     1501 gtcctctgta taagggaaca gaaatcaggc ttttggacat tcataccggt gagcagatat
     1561 atccgggaaa gaacggattt ggaagaaagg gagaaattca tgttcgcgga gaacaggtaa
     1621 tgaaaggata tttcaagaat caggaagcga cagaaaaggt tttgaaagac ggatggctga
     1681 atacaggcga tctgggaatg atgacatata acggatgtct taagattacc ggaagatcca
     1741 aggagacaat tgtgcttctc gggggagaaa atgttgagcc ggttcctttg gaaaacaggc
     1801 tcctggaatc tcccttaata gatcagtgca tggttgcagg tcaggatcga aaattcttaa
     1861 gtgcacttat tgttcctcgg atggaaaggt tcagagagta tgggagcagt ctttctgaac
     1921 tttctgaaaa tgaatctgta agaaagattc tgagagagga gcttaagaga cttgtgagca
     1981 cggagaacgg ctttaaatct tttgagaaaa tatccgattt ccgaattctt ccgaaatcct
     2041 ttgaggcggg tgatgagctt tctgcaaagc tttccataaa aaggcatatt atcacagaga
     2101 aatacagtca tctcattgat gatatgtata aaaaagcgta agttttcgga ttctgaccgg
     2161 gcagttcccg gtcagaggat ttctgtcaaa gtctgcagtt tgggtttttt ccctttgcct
     2221 ttgcctcatc gtaaagtttt ttggcgtcag gaagtttttc gttcaggttg ttcattcttg
     2281 tctgatcaga cgggtgagtg gacatgattt cgggaggttt cgatcctcct gcggcattca
     2341 tattcttcca tagattgatg ctttcactcg gatcaaatcc tgcctttgcc atgtactcaa
     2401 gtccgagtat gtccgcctct gtttcatgtt ttctggaaaa tggaagcata actccaaact
     2461 gagctcccgc accgattgcc cccataatct ttggatcatt cttggttgca gaaccgatgg
     2521 cttccattcc tttctgaaga agacctcctt ctgaaattct ttctgcaccg tgtcttgcaa
     2581 gtacatgagc cacttcatgt cccatgactg cagcaagctg atctgcggtt tttgccacag
     2641 gcagaattcc tgtatgcacg cctatctttc ctcccggcag agcaaaagcg ttcgcagtac
     2701 tgtcattgaa tactactact tcccagctct gaactccggt gctgtcggag atgactcctg
     2761 tgattgcatt tgctatgcat tttacatatg catttgtgtt cggatcagtt tctgccggag
     2821 tctttttttt cagatcctca aatgcctttg cacccatctg attcatttca ggatcatcta
     2881 cagtgatgat tctgtatctt cctgtcggtg aagtatattt ttccgggaga gctgaacagt
     2941 gaaacagtaa aactgcagca gtcatatatg gaatgatttt tttcattttc cctgccttaa
     3001 tggaaatttt ctaagttgaa tttttcttac tgcagggttt tctgtaa
//
LOCUS       DAOQGJ010000245         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19179_length_3046_cov_4.100635, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000245 DAOQGJ010000000
VERSION     DAOQGJ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19179_length_3046_cov_4.100635"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PK683_05095"
     CDS             complement(<1..655)
                     /locus_tag="PK683_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07856.1"
                     /translation="MKKIIGGAVLALLIGGSLQAQTQGELFRKPGVIGDSLSQGFFGV
                     TVEKKTQDWAYPVLVSKQAGSNVSYNTLKGPYLNFEDVLKGNCGPICIASSIIGGNDT
                     TVALPTHAGITGAEYTSVLRTSGKCEDITATKQEREWYWANWYTYTYRWITVQDCKDP
                     DKFHQYGLRDSGTQMEVMEKNKPSFLFGTAGANHVLCTALSTSLDCLDEARFKRDIRE
                     "
     gene            complement(896..1303)
                     /locus_tag="PK683_05100"
     CDS             complement(896..1303)
                     /locus_tag="PK683_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07857.1"
                     /translation="MKKNIKKIGLIFLLLLMPAVISAGTPDTQGTEINGNWAALANNA
                     KIEGRLFWLYLSIKDGQACMHNLYVKDGKCPSSCYSVNKGKFEAGKIDFSLTWSEKDK
                     ILVESWAVNGKPDGLYWVKYDLPKGCVHGQPTK"
     gene            1855..2511
                     /locus_tag="PK683_05105"
     CDS             1855..2511
                     /locus_tag="PK683_05105"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNH07858.1"
                     /translation="MRRNIRLLSLEDVEAELNYLDGAGSIQTSGLWTYYQILSHMNDA
                     VHFSMTGFPSVLPGFIRKTVGRYMFKKLIKTGIMKPGYLNPSAPKKREEGDARLMLQK
                     LRSTLNDFKNYNGEMSVHPIFDRLSKEDYTKLHSIHFSLHLSFAHPDFSIPKRDTTLP
                     PTKINDVPELKEEIIEDKKKKKKADSPKDSRSKSQKPKEKEKPDSKSKKKKSVQKKRK
                     "
     gene            2513..>3046
                     /locus_tag="PK683_05110"
     CDS             2513..>3046
                     /locus_tag="PK683_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNH07859.1"
                     /translation="MKIGIISGSHRSSSNSGKTAEFCRNVLTGKNIASYILNLERNRI
                     PQWEEEFWDKTNEWMKIWNPISSELQSCEGFIIISPEYGGMVPSALKNFFLFCSSELA
                     HKPGLIISVSSGRGGSYPVAELRMSSYKNSHICYIPEHVIIRDANNMLKTDGTASSDD
                     IYIQERIHYCLNILIGYS"
BASE COUNT          925 a          678 c          628 g          815 t
ORIGIN      
        1 tttcacggat gtctcttttg aatcttgctt catccagaca gtcaagtgaa gtgctcagtg
       61 cagtacagag aacatggttt gcgcctgctg tcccaaaaag aaaagacggc ttgttctttt
      121 ccataacttc catctgagtt ccagaatctc tgagtccgta ctgatgaaat ttatcaggat
      181 ctttacagtc ctgaactgta atccatctgt atgtataagt ataccagttt gcccagtacc
      241 attctctttc ctgcttggtt gcagtgatgt cttcacattt tccgcttgta cggagaacag
      301 aagtatactc agctccagtg atccctgcgt gagtaggaag agctacagtc gtatcatttc
      361 ccccgatgat ggaagaagca atgcagatcg gtccgcagtt tcctttcaga acatcttcaa
      421 agttcagata aggccctttc agagtattgt aggacacatt ggaaccggcc tgtttcgaaa
      481 cgagtaccgg atatgcccag tcctgtgtct ttttttccac agtcactccg aagaaaccct
      541 gactgagtga atcgccgatg actcccggtt ttctgaacag ttcaccttga gtctgtgcct
      601 ggagtgatcc tccgataaga agagccagca cggctccgcc aataattttt ttcatattgt
      661 gtcccccgat agttttcaga tctgccaaga atttcagcag taaatttcag aataacattc
      721 ctgtacaatg cttgcaagaa ataatttttt atccgggaaa aaagcggctc accgatccga
      781 ctgcagagaa ccctttattc gcagggaaca aaagaagcaa aaccaacatc agcaatgcag
      841 tgcagttcga cgagcaggaa aagcttctga acctgcaaag ccgaaccgct tcggactact
      901 tagtcggctg accgtgaaca cagcctttcg gaagatcata ctttacccag tacagtccgt
      961 ccggtttgcc gttcactgcc caggactcca caagaatctt gtctttctca ctccaggtaa
     1021 gggaaaaatc gattttgcct gcttcgaatt ttcctttgtt taccgaatag cagctcgatg
     1081 gacatttacc gtctttgaca tacaggttgt gcatacaggc ctgaccgtct ttgatggaga
     1141 gatagagcca gaataaacgg ccttcgattt ttgcattgtt tgccagcgca gcccagttcc
     1201 cgttgatttc agtgccttga gtatccggtg taccggcaga gattactgca ggcatcagca
     1261 gtaacagaaa aattaaccct atttttttaa tgttcttttt caaacgtcct ccattttttt
     1321 gacagccgat tctgaaacac tgcccaagta caacctgctc tgttcatttt catcctgtca
     1381 aaattcaatc ttagattaga cagctgtcag atctgagtca aacgaattaa aatggaaaaa
     1441 ttgcatatcg agaaattcag gaagaccgta ttaatactga ctccaagtct catcgttaat
     1501 ttttgagagt gttaaacatc atatacagtc atttgtttta cagataataa taacacgtat
     1561 aaacccgatc tgtttcgcag aatatggaaa gagcacaaca gatcagattt ttgaaaagac
     1621 ttcataccag taaacagctt tccttattcc ctcttgtaaa aacaagtgca ttaaataaat
     1681 aaaaaattga cacaggttca acgatcagat gcagcatttt ggataccggg cagaggctga
     1741 actttcggcc cgttcttcgg caccggaatg tcgcaggctg aatcaaaaat tatagacaaa
     1801 aggactttac tcagtgaagc atattctctt tcttgtctat attcgggagg gacaatgaga
     1861 agaaacatca gactgctgag cttggaagat gtagaagcgg aactgaatta tctggacgga
     1921 gcaggctcca ttcagacctc agggctttgg acctattatc agatattgag tcatatgaat
     1981 gatgctgtcc atttttcaat gacaggtttt ccatccgtcc tgccgggatt catccgaaaa
     2041 acggtcggtc gttatatgtt taaaaagctg atcaagaccg ggattatgaa gccgggttac
     2101 ctgaatccgt ctgcaccgaa aaaaagggaa gagggagatg ccagactgat gcttcagaaa
     2161 cttcgctcga ctctgaatga tttcaaaaat tacaatgggg aaatgtcagt tcatccgatc
     2221 tttgacagac tgagcaagga ggactacaca aaactccatt ccattcattt ttctctccat
     2281 ctcagctttg ctcatcctga tttcagcatt cccaaaagag acacgactct tccgcctaca
     2341 aagatcaatg atgtaccgga actgaaagaa gaaatcatcg aagacaaaaa gaaaaagaag
     2401 aaagcagatt ctccgaaaga cagccgaagc aaatcgcaga agcctaagga aaaagaaaag
     2461 cccgattcca aatcgaaaaa gaaaaaatct gttcagaaaa aaagaaaata atatgaagat
     2521 agggattata tcaggcagtc acagatccag ttccaactcc ggcaagactg ctgaattctg
     2581 cagaaatgta ctgacgggaa agaatatcgc ctcttacata ctgaatctgg aaagaaacag
     2641 aatccctcaa tgggaagaag agttttggga caagacgaac gaatggatga agatatggaa
     2701 tccgatctca tctgaactgc agagctgcga agggttcatc atcatttctc ccgagtatgg
     2761 aggaatggtg ccgtccgcac tcaagaattt tttccttttc tgctcttcag aactggcaca
     2821 taaaccagga ctgattattt ctgtctcatc aggaagaggc ggttcctacc ctgttgcaga
     2881 actgagaatg agcagctaca agaattctca tatatgttat atcccggaac acgtcattat
     2941 cagagacgca aacaatatgc tgaagacgga cgggacagct tcttctgacg atatatacat
     3001 tcaggaaaga attcattact gcctgaatat tctgatcgga tattcc
//
LOCUS       DAOQGJ010000246         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19345_length_3030_cov_2.456134, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000246 DAOQGJ010000000
VERSION     DAOQGJ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19345_length_3030_cov_2.456134"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PK683_05115"
     CDS             complement(<1..1032)
                     /locus_tag="PK683_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07860.1"
                     /translation="MIRLIMIFSLFSIGMLAGCTTKEKKIVGEDVIASDAKPQTFQKI
                     APLTSNALGARKNLHEKGWYFIPSSSESYQKVKKSGRMSFQTAKAMLFVSMKKRAQDY
                     PGELGNTMAEIWKVAGNDGRETYEKISKDIYDGTYEIVKWEVRSAKDLLKESGKSFLG
                     YASLGTVMKDDMKKTYSDMAEYNGHLGQNYASLSEMFEDTRKQSGDRIANAWKGAFSR
                     GAKEWKDNLAESGEQPNSALALWNVFKGYTLALKEVALSPVADTAVGAGQFLAGGTAA
                     STGTAATGIGQIIVTTGLTLVHAGKAGYRVLSPSMESGFLGTLGVASAVATVPTAIGA
                     EATYGFNQTL"
     gene            1666..2202
                     /locus_tag="PK683_05120"
     CDS             1666..2202
                     /locus_tag="PK683_05120"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH07861.1"
                     /translation="MRSLLSIYIFFALNISAKPLNWYEYPGRWFKNILSVPAKDSPAP
                     EIDAEDPAGNPVFLQNVEGEYILLSFWASWCPVCRKELPELDKLSVKFSSRGIKFLAI
                     NVNDSKEKAIDYINKNKSSLIYLLDTDDRTSEKYRIISFPSNFLIQKKGLKTVKVWEG
                     DIEMKTIEEYLEKLPYKK"
     gene            complement(2327..2635)
                     /locus_tag="PK683_05125"
     CDS             complement(2327..2635)
                     /locus_tag="PK683_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNH07862.1"
                     /translation="MLNTSPDSVRYDDKNLYILWKDGVESSFDLLTLRKNCPCATCRG
                     GHFGKIGAATGNITSVSLVSFRKVGRYALNLTWNDNHDTGIYSYDNLRAFADGKSSEL
                     "
     gene            complement(2635..>3030)
                     /locus_tag="PK683_05130"
     CDS             complement(2635..>3030)
                     /locus_tag="PK683_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07863.1"
                     /translation="YLKKSVSLKVIFSFGGSSLYTFRNRVFLALSLCTGKPPDSFAEI
                     IVSKRLIQFSSAFSESDSEFINRRILLDLKIRYKNLVSMNQKTVKLLKKYADLKGLNE
                     DDLKKEWMSLGQHEKSSKRQEILSSFLKK"
BASE COUNT          847 a          678 c          577 g          928 t
ORIGIN      
        1 cagtgtctga ttgaagccgt atgtcgcctc agcaccgatt gcagtcggca ccgtcgcaac
       61 cgcacttgcc actcctaagg ttcccaggaa tcctgattcc atactgggac tcaaaactct
      121 gtatcctgcc ttcccagcat gtaccaaagt caatccagtc gtgacaataa tctgaccgat
      181 tcctgtagca gcagttcctg ttgatgcggc ggttcctcct gcaagaaact gacctgcccc
      241 gactgctgta tcagccacag gagaaagagc aacctccttc aaagcaagcg tgtatccttt
      301 gaatacattc cagagtgcaa gtgcggaatt cggctgctcc cctgattcgg cgagattgtc
      361 cttccattcc tttgctcccc tcgaaaatgc tcccttccag gcatttgcaa tccggtcccc
      421 gctctgtttt ctggtatcct caaacatttc actgagagaa gcatagttct gaccgagatg
      481 cccattgtat tcagccatat ccgaataggt ctttttcata tcatctttca tcactgtccc
      541 gagggatgca tatcctagaa aagacttccc tgattctttc agaaggtctt ttgccgatct
      601 gacttcccat ttgacaattt cataagtacc atcatatata tctttactta ttttttcata
      661 agtctctctt ccgtcatttc ctgccacttt ccaaatttcc gccatagtgt tcccgagttc
      721 acccggataa tcctgtgccc tttttttcat ggaaacaaaa agcattgctt tggcggtctg
      781 aaaggacatt ctcccgcttt ttttcacttt ctgatagctt tctgaagaag atggaataaa
      841 gtaccatccc ttttcatgca ggttttttct tgcaccgagc gcattggatg tcaatggggc
      901 aattttctga aatgtctgcg gttttgcatc ggaagcaatc acatcctcgc ctacaatttt
      961 cttttctttc gttgtgcatc cggcaagcat accgatgctg aacaaactga atatcattat
     1021 cagccgaatc ataaaatctc ctgaattttg tattttccag ttttgaacat tcttcagatt
     1081 tttctaatct tttttatcat gaatttctta aaagtcgttt ccgcagcagt tctgctctac
     1141 cggacacaat tcagcttccg cagtttctga tcggtgcatt tctccgcagc ggagccggat
     1201 caggttctgt ttttagagag attacacata gactttttac gctggaaaac cttgattttt
     1261 cctgtccagc aaggctgaat agtcaaagat ttttgaaaat gagtataatt ctaaaataat
     1321 gatctttttc agaaaaacag gatacggact ttgaaacgca gttttgttca gttccggctg
     1381 caattccgga aaccatctga atcagtcgga gtattcaatc attatactgt aattaaataa
     1441 atcaaccggg aaaactcaat gaatcgttca ccttagagac cgtgcaaaag ctctgttttt
     1501 atgcagatct caaaagaaat aaaatgaatt ggatctgagg actcgcatac gtggacgccg
     1561 ctgttcgaaa agtcgtaaaa tgaatcagga actgttaaat tctgcccttg acttttctga
     1621 aaaacaatct gatctggtaa ggactgcagg ggaaaatcat catatatgcg atctttactg
     1681 tcaatctaca tattttttgc gctgaacatt tcagcaaaac cgctcaattg gtatgagtat
     1741 ccgggacgct ggtttaaaaa catactttct gttcctgcca aggacagtcc tgctccggag
     1801 atagatgccg aagatcctgc cggaaacccg gtttttcttc agaacgttga aggcgaatat
     1861 atcctccttt cgttttgggc ttcttggtgc cctgtatgca gaaaggaact gcctgaactg
     1921 gataaactga gtgttaaatt ctcctctcgc ggaataaaat ttcttgcaat aaacgtaaat
     1981 gattctaagg aaaaagcgat cgactatata aataaaaata aatcatcctt gatttactta
     2041 ttggatacgg acgaccggac atcggaaaaa tacagaatca tttcttttcc ttccaatttt
     2101 ctgattcaaa aaaaaggttt gaaaacagtc aaagtctggg agggagatat cgaaatgaaa
     2161 acaatcgaag aatatttgga aaaacttccc tataaaaaat agacagtctt gaaaaacaga
     2221 agcaatattt actgtctgca ttcctaaaaa aacaaaatca ggcgactgat ctctgagtca
     2281 gtctgtattt tcttctgaaa cctattttgt aattgtataa attattttaa agttctgagg
     2341 acttcccgtc tgcaaatgct ctcaggttgt cgtaggaata tatccctgta tcgtgattgt
     2401 cattccaggt cagattcagt gcataacggc ctaccttgcg gaaggaaaca aggctcacgg
     2461 atgtaatatt gccggtcgct gctccaatct ttccaaaatg ccctcctctg caggtcgcgc
     2521 agggacagtt ctttcggaga gtcagaagat caaaggagga ttccaccccg tctttccaaa
     2581 gaatataaag atttttatca tcatatctta cggaatcagg ggaggtattc agcattattt
     2641 tttcaggaaa gaagaaagaa tttcctgtct tttggaagac ttttcgtgct gaccgagaga
     2701 catccattct tttttcagat catcttcgtt cagacctttc aagtctgcat atttctttaa
     2761 cagctttaca gttttctggt tcatggaaac taggttttta taccttattt tcaggtcaag
     2821 gagaatccgc ctgtttatga attccgaatc agattctgag aaagcactcg aaaactggat
     2881 cagtcttttt gagacaatga tctctgcaaa actgtccggg ggcttccctg tacagagtga
     2941 aagtgccaga aaaactctgt ttcggaacgt atacaatgaa ctgcctccga aactgaaaat
     3001 gactttcagc gaaacagact tcttaaggta
//
LOCUS       DAOQGJ010000247         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19348_length_3029_cov_4.188971, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000247 DAOQGJ010000000
VERSION     DAOQGJ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19348_length_3029_cov_4.188971"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PK683_05135"
     CDS             complement(<1..540)
                     /locus_tag="PK683_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNH07864.1"
                     /translation="MKDPVLGESPQAKKIRDKISLLKGRKFDLLLSGENGTGRDTAAD
                     FLSSANSELPLFKIDCRDWRNDFDFRISSGKFKGKEAAFEENFYFDRLDDLSSEGQGF
                     LLRLIEERTARYSEKYNIKLYGQIFFSASEGLSELVKKKKFRDDLFSRISAVRISLVP
                     IRERRQDIPLYVNLFLEELS"
     gene            complement(537..2255)
                     /locus_tag="PK683_05140"
     CDS             complement(537..2255)
                     /locus_tag="PK683_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07865.1"
                     /translation="MRSKIGEKLQVNSILRLTFFGGFAGFGILGYIYPILKLYLYTIS
                     SLSFLLFLYTVRVYRSWEFLQTRVDSLVSFLKREKKRSEHQLEFLHKGETVEENPNSF
                     WKDLDVFGKKSLHTYLDTTISAQGSEKLKSLLLQKELLSSEDIMKRQKLIGSLSGAKY
                     RLLKILRLFYQASESEGYSKISPAKHLVPPSHYYEKHNIIGMIFRPFVILQFIIAAAV
                     FMMDISRIPFSFLILNTLFMAVGFRERNHLLKSWKKFSETFAGLKELAFYLNRIGLLE
                     NSDLRKVFGRTESYERRFQYLDAPLFRMLLNVIFLWEFWEIKSLEKFYLHYKSSVLQI
                     IEAIEDLDSILVFANFHLHNPDAVFPEISENTELEAKKLYHPLIPAEKRVSNAVDYMK
                     GGELVLITGSNMSGKTTYIRSIGLNVLLAMCGSPVLAEKMKLPVMKILSSIRNEDSLS
                     EGISFFYSEVKKLRFILETVSDKNNRYIVLIDEVLKGTNTRERLIASSLILQKLIQSG
                     AVSFVTTHDLALAKKKNAKIRLRHFKEDVDKEGKMIFGYKIQKGLVSSTNALRVLKSE
                     VPDLFK"
     gene            complement(2299..2535)
                     /locus_tag="PK683_05145"
     CDS             complement(2299..2535)
                     /locus_tag="PK683_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002151442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07866.1"
                     /translation="MTIKMVNKTGSRYGRFVAYEYGQDLFGYIYLDKIKGREKGKVVD
                     SWILEDLASLVKTLDLELYKREVENYENTSHWNA"
     gene            2725..2934
                     /locus_tag="PK683_05150"
     CDS             2725..2934
                     /locus_tag="PK683_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07867.1"
                     /translation="MYYEEMDTEVKSVFYKTKESFANLASETQMPAFGLIARNLLDDE
                     QYQKFQKIRNKKFKTESQLSAFYQD"
BASE COUNT          898 a          622 c          619 g          890 t
ORIGIN      
        1 ggacagctcc tcgagaaaaa gatttacata caggggaatg tcctgccttc tttcacggat
       61 gggaaccagg ctgatacgta ccgctgatat gcgcgaaaaa aggtcatctc tgaatttctt
      121 ctttttgaca agttctgaca gtccttcaga tgctgaaaag aatatctgcc catataattt
      181 aatattgtat ttttcagagt atctggcagt gcgttcttct atcagcctta aaaggaatcc
      241 ctgtccttca gaactcagat catccagtct gtcaaaatag aaattttctt caaatgctgc
      301 ttcttttccc ttgaattttc ctgaggagat ccgaaagtca aagtcgtttc tccagtccct
      361 gcagtcgatt ttaaaaagag gtaactcgga attggctgag gagagaaagt ctgcagcagt
      421 gtcgcggcct gttccgtttt ctcctgataa tagaaggtcg aattttctgc cctttaagag
      481 ggagatctta tcacggattt ttttcgcctg aggagattct ccaagaactg gatctttcat
      541 ttgaacagat caggcacctc tgacttcaat acccgtaaag cgttggtact gctgacaaga
      601 cccttttgaa tcttatagcc gaaaatcatc tttccttcct tgtccacgtc ttccttgaaa
      661 tgtctaagcc tgatttttgc atttttcttc tttgccagag ccagatcatg agtagttacg
      721 aaggacacag ctccggactg gatgagcttc tgaagaatca gagaggatgc aatgagtcgc
      781 tcgcgtgtat ttgttccttt cagaacctca tcgatcagaa caatataacg attgtttttg
      841 tcggataccg tttcaagaat aaaacgaagc ttctttactt cagaataaaa gaaagagatc
      901 ccctcactca aagaatcctc atttctgatc gaagagagga ttttcataac aggaagtttc
      961 attttctcgg caagtacagg tgagccgcac atcgccagaa gaacattcag cccaatcgac
     1021 cgaatatatg tggttttccc ggacatatta gagcctgtaa tcagaacaag ttctcctccc
     1081 ttcatatagt ctacagcatt ggaaactctt ttctctgcag gtatcaaagg atgataaagt
     1141 tttttggctt ccagctcggt gttctcagag atttccggaa aaacagcatc agggttgtga
     1201 agatgaaaat ttgcaaatac aagaatcgag tccagatctt caatcgcttc gattatctgc
     1261 agaacggatg atttataatg aagataaaat ttttctaatg attttatctc ccaaaattcc
     1321 caaaggaaga ttacattcag aagcattctg aaaagaggag catccagata ctgaaatctt
     1381 ctttcataag attcggttct gccgaagacc ttgcgtagat cggagttttc caatagaccg
     1441 attctgttca gataaaaagc aagttccttc agccctgcaa atgtctcaga aaactttttc
     1501 cagcttttca gaagatggtt cctttcacgg aaacccactg ccataaagag tgtattcaaa
     1561 atcagaaaac tgaacgggat tcgtgaaata tccatcataa agaccgctgc tgcaatgata
     1621 aactgcagga ttacgaacgg tcgaaatatc attccgatga tattatgctt ttcatagtag
     1681 tgagaagggg gaacgaggtg ctttgccggg gaaattttgg aatacccttc actttcggat
     1741 gcctgataaa atagcctcaa aattttcaac aggcgatact ttgctccgct gagactgccg
     1801 atcagcttct gccttttcat aatatcttcg gagctgagga gttccttctg aagaagcagg
     1861 cttttcagtt tttcacttcc ctgggcagat atagttgtat ccaggtaagt gtgcaggctt
     1921 ttttttccaa aaacatccaa gtctttccag aaactgttgg ggttttcttc cactgtctcc
     1981 cctttgtgaa ggaactccaa ttggtgttct gaacgttttt tctccctttt caggaaggat
     2041 accagagagt ctaccctagt ctgcaggaac tcccaagagc ggtaaactct gacagtatac
     2101 aggaagagca gaaagcttaa tgaagagata gtatacaaat aaagtttaag aattgggtaa
     2161 atatacccta gtattccaaa accggcaaat ccaccgaaga aagtgagacg aagaatactg
     2221 ttaacttgaa gtttttctcc aatttttgaa cgcactttgg agagtttttt cagtcttata
     2281 gataggatga aagggaattc aggcattcca gtgagatgta ttttcgtagt tttcgacctc
     2341 tctcttgtag agttcgagat cgagggtttt cacgaggctc gcgagatctt cgagaatcca
     2401 actgtcaaca acctttcctt tctcgcgacc tttgatttta tcaaggtaaa tataaccgaa
     2461 caggtcttgg ccgtattcat aagccacaaa tctgccgtat ctgcttccgg ttttattcac
     2521 catcttgatg gtcatagcat agacaatata tttatgcgta cttacacgta caagattttt
     2581 ttttacaatc aaggattttt ttttcatacc gaaaaagaaa tggggggtat ctgaccttca
     2641 tggaggaagt tcagccccaa agaatacaat ctaatcagca aaaactttag atttaagctt
     2701 acatacagcg catggaggcg atttatgtat tacgaagaaa tggacacgga agtgaaaagt
     2761 gtcttctaca aaacgaagga gagtttcgcg aacttggcaa gcgaaactca aatgccggca
     2821 ttcggcctta ttgcaagaaa cctgcttgat gatgagcagt atcagaaatt ccagaaaatc
     2881 agaaacaaaa aatttaagac tgaatctcaa ttgtctgctt tttatcagga ttaggcgggg
     2941 gattcggtgt gcaaaaaaaa gcacaccgac cgggctttcg gccaatgtca ttaagttaag
     3001 gagccaaaag tacgacagga atattatga
//
LOCUS       DAOQGJ010000248         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19378_length_3027_cov_3.427322, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000248 DAOQGJ010000000
VERSION     DAOQGJ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19378_length_3027_cov_3.427322"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK683_05155"
     CDS             <1..357
                     /locus_tag="PK683_05155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07868.1"
                     /translation="IGNSLSPKNRMRVILSLDEILSNIIEHGFPEKKESFIEAEMTLY
                     PDRIICVFTDEGIPFNPLSAPEVNINEHLDKGEDGGMGIHIVRKIMKASYEFTSDRKN
                     RLTLIKELKGEDNENV"
     gene            344..>3027
                     /locus_tag="PK683_05160"
     CDS             344..>3027
                     /locus_tag="PK683_05160"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH07869.1"
                     /translation="MKMFDWRKIIRGIREKLSLFIILFIAVIVVITSAVNFIQQKETL
                     IANIEAEIRPAEDYIHIEVENIERIAESLILIERFKENVKVKKQMLQQYMTRQYFAKE
                     KEYKFLGMKIKAGGILGNIVGKDIYSKMGDTFFSEYLSEDDIKDLESNIKLNLKDKNG
                     KVISDQEFIDIKKAAQKAAQAADSLKNAAQVSEYEGKPDSKNSEKQTALLNFNKFNSE
                     LRNKIFVYFQSEQKRKLDALKFPIEKIRIQSYSIVINENTIDAAVGFDTSGFLKKRNS
                     GKKKSGNPSADDDSAPAIIRKDLTDFLEPALAEFLAVLKGPDSGEKTLKEKFLSLFRK
                     QKTEEDALSEKTEFKFNDRIYRTHYSVNYKNLPVIRRAKLLTEKKTDDWKDYFEKEKT
                     VQTKFRDLIPKIRTRLDELKKEKVPVKPSMDPLFIRLYQEYKSLIRERDRLFDEALKK
                     MTYITDYETKNPDVFDIEMLLVKEAVKNLRNVSLYETVVLKHKSSQYDYEDYIEADRT
                     AKNAEARKWDLLRKWIMNADSEKATPELKAAMAGGTLSKSRSEAEEMMWYLDSKPLFS
                     EDGDHLAAKLVNDNKIGFTRTVTDMQEGYDSIRDSLFKLISFMVSIGLLGIVSAYYFS
                     GRVVKKIKKIINSADELGKGNLDTKFEHGGSDEFGILTTALNRMTADLKHRHEMMMEY
                     AAAEDIQKGLLPVEMPSNSSGVLEFGNFYKSMSGVGGDYYDYMNSMNGKTAFCIGDVS
                     NHGIGPALVMVALRSHLHSLIRSGETDLLKILLLLNEQAYQDTPSHIFVTFFLGMFDP
                     KDGTVTYCSCGHSKPALYKAESGKVELLGSGGMPLGAIDNDIFETALEVCTVNLKKGD
                     IFFQYTDGLNEAMNSAGELYGTDRMENIIRNASKDNVE"
BASE COUNT         1028 a          576 c          679 g          744 t
ORIGIN      
        1 ataggaaatt cattaagccc taaaaacaga atgagggtta ttctttctct ggatgaaatc
       61 ctgtcaaata tcatcgaaca cggatttccc gaaaaaaaag aatccttcat agaagcggaa
      121 atgacacttt atccggacag gataatatgt gtatttaccg atgagggcat cccgttcaat
      181 cctctgtcgg ctcctgaagt gaacatcaat gaacacctgg acaagggcga agacggcgga
      241 atgggaatcc atattgtaag aaaaataatg aaagcatcat acgaatttac atcggacaga
      301 aaaaacagac tgactcttat caaggaactg aaaggcgaag acaatgaaaa tgtttgactg
      361 gagaaaaatc atcagaggaa tcagagagaa gctgtccctt ttcataatac tttttattgc
      421 tgtcattgta gtcataactt cagcagtgaa tttcatacag cagaaggaaa ctctgattgc
      481 aaatatcgag gctgagatca gacccgccga agactacatt cacattgaag tggaaaacat
      541 agaaaggatt gctgaaagtc ttatcctgat tgaacgcttc aaggaaaacg taaaagtcaa
      601 aaagcagatg ctgcagcagt atatgacacg gcagtatttc gcgaaggaaa aagagtataa
      661 atttctggga atgaaaatca aggcaggagg cattcttgga aacattgtag gaaaggacat
      721 ttattcaaag atgggagata catttttttc agagtatctt tctgaagatg atatcaagga
      781 tctggaatca aatataaaac tgaacctgaa ggacaagaac gggaaagtca tatcggatca
      841 ggaatttatt gacatcaaaa aagcagcaca gaaggcggcc caggctgcgg acagtctgaa
      901 aaatgccgct caagtatccg aatatgaagg aaaaccggac tcgaagaaca gtgagaagca
      961 gacagcactt ttaaatttca ataaattcaa ttctgaactt agaaataaaa tatttgtcta
     1021 ctttcagagc gaacagaagc ggaagcttga tgccctgaag tttccaatcg agaaaatccg
     1081 gattcagtcc tattcgattg taattaatga aaacacgatt gatgctgcag tcggatttga
     1141 cacttccggc tttctgaaga aacgaaattc aggaaagaaa aaatccggga atccttcagc
     1201 agacgatgat tctgccccgg caatcatcag aaaagatctc actgattttc ttgaaccggc
     1261 gcttgctgag tttctggctg ttctgaaagg gccggattcg ggagaaaaaa cacttaagga
     1321 aaaattcctt tctcttttca gaaagcagaa aacggaagaa gatgcccttt cggaaaaaac
     1381 tgaattcaaa tttaatgata gaatataccg gacacattat tcagtcaatt ataaaaacct
     1441 tcctgtcatc aggcgtgcca agctgcttac ggaaaagaag acagacgact ggaaagacta
     1501 ctttgaaaag gaaaagacag ttcagacaaa attcagagat cttatcccta aaataagaac
     1561 aagacttgat gaacttaaaa aggaaaaagt ccccgtcaag ccttctatgg atccgctttt
     1621 tatcaggctt tatcaggaat ataagtcttt aatcagggaa cgggaccgac tctttgatga
     1681 agcactgaaa aaaatgacct atattactga ttatgagaca aagaatcctg atgtattcga
     1741 tatagaaatg ctgcttgtaa aggaagcagt caaaaatctg cgcaatgtgt cgctttacga
     1801 aacagtagtg ctcaagcaca agtccagtca gtatgattat gaagactata tagaggcgga
     1861 cagaacagca aagaatgcgg aagcccgaaa atgggatctt ctgagaaagt ggataatgaa
     1921 tgccgattct gaaaaggcga cccctgaact gaaggctgca atggcagggg gaactctttc
     1981 gaaaagcaga tcagaggctg aagaaatgat gtggtatctg gactctaaac cgcttttttc
     2041 cgaggacggg gatcatcttg ctgcaaaact tgtcaacgac aacaagatag gatttacccg
     2101 cacagtcact gatatgcagg aaggatacga ttccatcagg gacagtctgt tcaagctgat
     2161 ttcatttatg gtttccatag gtcttctcgg aatcgtatcg gcatattact tctccggcag
     2221 ggttgtcaaa aagataaaga aaattatcaa ttctgcagac gaactcggca agggaaatct
     2281 ggacacaaaa tttgaacatg gaggaagcga tgaattcggc attcttacaa ctgctctgaa
     2341 cagaatgaca gcagacttga agcacagaca tgaaatgatg atggaatatg cggctgctga
     2401 agacattcag aaaggtcttc ttccggttga aatgccttca aattcatccg gggtgcttga
     2461 attcggaaac ttctacaaat caatgtccgg cgtcggcggt gactattacg attatatgaa
     2521 ttcaatgaac ggaaagacag cattctgcat cggggatgtt tccaatcatg gaatcggtcc
     2581 tgccctcgtt atggtggcat tgcgttctca tctgcactca ctgatcagat cgggagaaac
     2641 ggatcttctg aaaatccttc ttcttctgaa tgagcaggca taccaggaca ctccttccca
     2701 tatatttgtc acttttttcc taggaatgtt tgatccaaag gatggaacgg tcacctactg
     2761 cagctgcggt cattcaaagc ctgcacttta caaagcggaa tcaggcaaag tggagctgct
     2821 cggttcaggc ggtatgcctc ttggagcaat cgacaatgat atttttgaga ccgcactgga
     2881 ggtctgtacc gtaaatctga aaaaaggcga tatattcttt cagtataccg acggactgaa
     2941 tgaggcaatg aattctgcag gtgaactcta tggaactgac cgaatggaaa acattattcg
     3001 aaacgcttct aaggacaatg tggaaga
//
LOCUS       DAOQGJ010000249         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19402_length_3025_cov_3.194949, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000249 DAOQGJ010000000
VERSION     DAOQGJ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19402_length_3025_cov_3.194949"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..304)
                     /locus_tag="PK683_05165"
     CDS             complement(104..304)
                     /locus_tag="PK683_05165"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNH07870.1"
                     /translation="MSNENIGSTDRIIRFIAGIVLAGAGFAMQSPVSYVLWGLGAIML
                     GTAAIKVCPLYIPFGISTCAKK"
     gene            601..1578
                     /locus_tag="PK683_05170"
     CDS             601..1578
                     /locus_tag="PK683_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH07871.1"
                     /translation="MIKVTFLGLENSISSFITGPLDLFYHTGRIWNAINGSTEEPVFE
                     AELVSIDGRQITCHNGIVLNPKKSIFDSEKSDLIVISSASFDIEWTLKKYRPVIPFLK
                     EKHGQGIPIASLCIGAFLLADSGLLNGKKASTHWGFCNLFEEMYPDVRLDKRGLISED
                     SGLFTSGGANSGLDLTLYLIEKFSGHDAAVNLAKSFVLEPNRNYQSPFSKFIGRRKHN
                     DKEILYAQEWIEKNYTHFRQFNELAEKLGMSQRNLNRRFKEATGISPVDYLQFTRIES
                     AKIYLERGNETVQEITYLVGYEDISFFRKVFKKIAGVSPNQYRQMYRGH"
     gene            1580..2863
                     /locus_tag="PK683_05175"
     CDS             1580..2863
                     /locus_tag="PK683_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HNH07872.1"
                     /translation="MKNLSLKILEKFPDPFVFAVLMNAFLCILSFFYFGVSFQNIASA
                     FRTGYFSLFSFSMQMALILLFGLTLAGSESFEKLLDRAAVHVSSPLTSVLAATLVSVV
                     LSWLNWGFGLILGTVFAVRLAQKNRNTDFRLLVSCIYSGFIVWHGGLSGSIPLTVSTE
                     GHSLQSKIGIIPLQNTVFSSFNLSLVLAVGAALSMANVFILSRIEFPLIAELPSEKKE
                     NPSSENGLLGSRIFSVLTAGAGTVLFISGFKSLDLNSVILILFLLCLLLHRSAESFLK
                     KAQQSIHSVTGILVIFPLYGIIMSLLNIPLSDGSTMSGKIAEMMSGIADQTTYPFLTF
                     ITAGILNLFIPSGGGQWAVQGPSAAEAASRLGADISRTVLAVSWGDAWTNLIQPFWAV
                     PILSVSGLKVRDILGFCIIQLIVSGFIISFWFLVF"
BASE COUNT          804 a          660 c          642 g          919 t
ORIGIN      
        1 ttcctgtaaa caatggtttt tttctgaaat ggatcaatca gccaggcgag agaactgccc
       61 cgatctgcag tcaatagatc gaagcatctt attttcaaaa cgtttacttt tttgcgcagg
      121 tactgattcc gaaaggaatg tacagaggac ataccttgat agctgctgtt ccaagcataa
      181 tcgctccgag gccccagagc acataggaaa ctggactctg catagcaaac cctgcacctg
      241 cgagcactat tcctgcaata aatctgatga ttctgtctgt tgatccaata ttctcgtttg
      301 acataaaaaa ctccttattc ataaccgttc caatctgaac cggtcatttt aatctgaatc
      361 ggttcgagct tcaggaggtt ttccgcttcc cgactgattc tttaaacaga ataacagatt
      421 tctgaaacag tgcaagagtc tattcggaca aaagagaatg agtatctgga aactgttttt
      481 ttctgaaaaa gtatttcatt tacagtcaga ttcaaatttg ctgtccagta atgaatttaa
      541 ttcactgaaa agcagggccg caatgccctc ttcagtccga ttggatctgt ttgaacaaat
      601 atgataaaag tcacatttct gggtctggaa aattcaatca gttcattcat cacaggaccg
      661 ttggatctgt tttaccatac aggaaggatc tggaatgcaa tcaacggcag caccgaagaa
      721 ccggtgtttg aggctgagct ggtttcgatt gacggtcggc agattacctg ccataatggg
      781 attgttctga atccgaaaaa atccatcttt gattctgaaa aatcagatct cattgtaatc
      841 tccagcgctt ccttcgatat tgagtggact ttaaaaaaat acagaccggt cattccgttt
      901 ctgaaggaaa aacacggaca gggaattccg attgcaagtc tctgcatcgg tgcctttctt
      961 ctggcggact caggccttct gaacgggaaa aaggcatcca ctcactgggg gttctgcaat
     1021 ctgttcgagg aaatgtatcc tgatgtgaga cttgacaaaa gaggactcat atcggaggat
     1081 tccggtcttt tcacttccgg cggtgcaaac tccggactcg atctcactct ttacctgatt
     1141 gaaaaatttt cgggacatga tgctgcagtg aatcttgcaa aatcattcgt gcttgaaccg
     1201 aacagaaact accagtctcc cttttcaaag ttcataggca gaagaaaaca taatgataaa
     1261 gagatacttt atgcacaaga atggatagaa aaaaattata ctcatttccg gcagttcaat
     1321 gagctggcag agaaactcgg aatgagtcag aggaatctga acagacggtt caaggaagca
     1381 actggaattt ctccagtaga ttatcttcag tttacgcgga ttgaatctgc aaaaatttat
     1441 ctggaaagag gaaatgagac tgttcaggaa attacttatc tcgtcggata cgaggacata
     1501 agctttttca gaaaggtgtt caaaaaaata gccggagtca gtccgaatca gtacagacag
     1561 atgtacaggg gacattaaaa tgaaaaatct cagtctgaaa atcctggaaa agtttcctga
     1621 tccgtttgta ttcgcggtcc ttatgaatgc ctttctatgc attctcagtt ttttttattt
     1681 cggtgtatcc tttcagaata tcgcctcagc attcaggaca ggatattttt cccttttttc
     1741 cttttcgatg cagatggctc tcatactgct tttcggtctg acacttgcag gatcagaatc
     1801 cttcgaaaaa cttctggaca gagctgctgt gcacgtcagt tctcctctga cttcggtact
     1861 tgctgcgact ttggtatcag tggttttaag ctggctcaac tggggattcg gactgatact
     1921 cggtacggta tttgccgtca ggcttgcaca gaaaaacaga aacactgatt tcagacttct
     1981 tgtctcctgc atctattccg gtttcattgt atggcacgga ggactttccg gctccattcc
     2041 tctgactgtg tccacggagg ggcattctct tcagtcaaaa atcggaatca taccgcttca
     2101 gaacactgtt ttctccagtt tcaatctgag tcttgttctg gcagtcggtg cagctctttc
     2161 tatggcaaat gttttcattc tttcgagaat tgaattccct cttattgcgg agcttccttc
     2221 tgaaaaaaaa gaaaatcctt cttcagaaaa cggtcttctc ggaagcagga ttttttctgt
     2281 tctcactgca ggtgccggga ctgttctttt tatctcagga ttcaagtctc tggatctcaa
     2341 ttcggtcata ctgatcctgt ttcttctctg tcttcttctc cacagatctg cggaaagttt
     2401 tctgaagaaa gcacagcagt caattcattc agtcacagga attcttgtga tatttcctct
     2461 atatggaatc attatgagtc ttctgaacat ccctctttcg gacggaagca caatgtcggg
     2521 aaaaattgca gaaatgatgt ccggaattgc agatcagacg acatatcctt ttttgacttt
     2581 catcactgca ggaattctga atcttttcat tccttcagga ggcggtcagt gggctgtaca
     2641 gggaccatct gcggctgagg cggcatccag actgggagca gacatttcga gaacagttct
     2701 tgctgtttcc tggggtgatg cctggacaaa tcttattcag cctttctggg cagttcctat
     2761 cctgtctgtc tcagggctca aggtgagaga catcctcggg ttctgcatca ttcagctgat
     2821 cgtaagcggt tttattatct ctttctggtt tcttgtattc tgaaacagat tcaataagcc
     2881 atcattcctt ttgcattgct ggaacaggga gactttgctt cgggtcagag ttctgcatac
     2941 tgacaagaaa ctcagtcagg cctgttcttt cggatctttc aattactgat aaataatgac
     3001 cacttttccc tgccctgcat ttccg
//
LOCUS       DAOQGJ010000250         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19442_length_3023_cov_2.358154, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000250 DAOQGJ010000000
VERSION     DAOQGJ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19442_length_3023_cov_2.358154"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..458)
                     /locus_tag="PK683_05180"
     CDS             complement(72..458)
                     /locus_tag="PK683_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07873.1"
                     /translation="MKILFILIFFILHCGSVYFIKNDAPVLTDEELNLLKSRKIGLIG
                     FSPFRISKFSEIHTAVPETVNDPAYRKMLLWLNKNGKKAEYNENMFLMFTGGKYAKVL
                     RALSGEIPLLHRKHSDLRFSFTAGHR"
     gene            620..745
                     /locus_tag="PK683_05185"
     CDS             620..745
                     /locus_tag="PK683_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07874.1"
                     /translation="MNHYPALLMDLHSREIAGSAVSEKNDTELVLAALSEALKYR"
     gene            complement(784..2118)
                     /locus_tag="PK683_05190"
     CDS             complement(784..2118)
                     /locus_tag="PK683_05190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH07875.1"
                     /translation="MQFDNRYEQIFNYSPLGMVIYNNDCIIQDVNPAFLEILECSRIE
                     LISASFTEFMHPEDVFNFLSLTEAQFSENIQGFKTEIRFQKKNGQTVWVSISAVKLSS
                     EKDTGLAIAENITERITIESALTHQTEKLVNEMQKSRLMNEKETAPSENSLEQKRILM
                     IEDDVLLKKTVARVLEKNGLLFFDAGTIQEALQMMSEDTFDLIILDLNLPDKTGYDLL
                     EELEENTRPPIIICSSDSKKENYPIAFRMGADDYMEKPLDMQELLIRIKALLRIQQKL
                     VLAKFASNTDEMTELHNGRSFKNYFDELIEESRNEGRQFAVISIDINSLKPVNDEFGH
                     AFGDVLIRNTADAIRKSAPENGFCARIGGDEFAVLIPEADLRQAQEFIYRLRNTLPDY
                     RFQAKKKEIEPSVSIGYAFFPENGNTKEELLHYSDVSMYKNKAIIKAQMRIK"
     gene            complement(2159..>3023)
                     /locus_tag="PK683_05195"
     CDS             complement(2159..>3023)
                     /locus_tag="PK683_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH07876.1"
                     /translation="TAANLFIPNKILDSVVFGKIREALGGELKGTISGGGALPAHVDE
                     FFNVLGIPVYEGYGMTECSPIISVRQKDKIIQGSVGVSPPGTEIRILNDQGEEVPVGH
                     MGIIHVKGPQVMKGYYKNQEATDKVLKNGWLNTGDLGFISFNGTLSVRGRVKDTVVLL
                     GGENVEPVPIENLLLQNALIQNVMVVGQDQKSLGALIYPNLERLVEAGFQIKPGEDLN
                     KNAKVVTHFSKIIKDTISSENGFKGFEKVTDFRFLPKPMEVGDEMTNLYKMKRNVITD
                     KYANLIKEIYS"
BASE COUNT          759 a          688 c          617 g          959 t
ORIGIN      
        1 ttccccagtc cgtttaggta atttccctga ttgaagacaa ggaactctct gcatttgtcc
       61 ggctgcggga attacctatg tccggctgtg aatgaaaaac ggagatctga atgtttcctg
      121 tgcagaagcg gaatttcacc gctaagggcc cgaagcactt tcgcatattt tcctcctgta
      181 aacataagaa acatattttc attatactca gctttttttc catttttatt cagccagaga
      241 agcatcttcc ggtatgccgg gtcattcact gtttcaggca cagcagtatg aatttcactg
      301 aattttgaga tccgaaacgg agaaaaaccg atgagtccaa ttttccggct tttcagcagg
      361 ttcagttcct catctgtcag aacaggtgca tcgtttttta taaaatacac tgaaccgcag
      421 tggagaataa aaaagatcag aatgaataat attttcattc tgcaaggttc tcataaaaat
      481 ctttttcctg caataggcag tttccctaac gtgccgggca gttaccgcag tttgccgggg
      541 agcttccctg ccaaggaccg atcaggggga ttccctgtgt cttgatcgga aattctgaag
      601 atgttttatt tctaagggag tgaatcatta tccggcactg cttatggatc ttcacagcag
      661 agagattgcg ggaagtgctg tctcggaaaa aaacgataca gagcttgtgc tggcagcgct
      721 ttcggaagca ctgaaataca gatgaaatcg tattttataa tgattttgca attaaaatga
      781 gagctatttt atcctcatct gcgccttgat gatggccttg ttcttgtaca tggatacgtc
      841 tgaataatgg agaagttctt cttttgtgtt cccgttttcc gggaagaatg catagccgat
      901 ggaaacagac ggttcaattt cttttttttt ggcctgaaat cggtaatcgg gcagggtgtt
      961 tctgagccgg taaataaatt cctgagcctg ccgaagatcc gcttcaggaa tcaggactgc
     1021 aaattcgtct cctcctattc ttgcacagaa tccgttttca ggagcacttt tgcggattgc
     1081 atctgcagta ttccgaatca gtacatctcc gaacgcatgt ccgaattcat cgttcaccgg
     1141 tttcagactg ttgatgtcaa tggatattac ggcaaactgt cttccttcat tcctgctctc
     1201 ttcaatcagt tcatcaaaat aatttttgaa ggatcttccg ttgtgcagct ccgtcatttc
     1261 atctgtattg gatgcaaatt tagccagcac gagtttctgc tgtatgcgca gaagcgcctt
     1321 gattctgatc agaagttcct gcatatcgag aggcttttcc atataatcat ctgcacccat
     1381 acggaacgca atcggataat tttccttctt ggaatctgaa gaacagataa taatcggtgg
     1441 tcttgtgttt tcttccagtt cttccagaag atcatatccg gtcttgtccg gaagattgag
     1501 atcgagtatg atgagatcaa atgtatcctc gctcatcatt tgaagggcct cctgtattgt
     1561 gcctgcatca aaaaaaagca gaccgttttt ctcaaggacc cgtgcaacgg tttttttcag
     1621 taaaacatca tcctcaatca tcagaattct tttctgttcc agcgaatttt ctgaaggagc
     1681 agtttccttt tcattcatca gtcggctctt ctgcatttca tttacaagtt tttctgtctg
     1741 atgcgtgaga gcagattcga ttgtgattct ttcagtgatg ttctccgcaa ttgcaaggcc
     1801 ggtgtccttt tcggaagaaa gctttactgc ggaaatggaa acccagactg tctgaccgtt
     1861 ctttttctga aaccgtattt ctgtcttgaa cccctgaata ttttcactga actgagcttc
     1921 tgtcagactt aaaaaattga atacatcctc cggatgcata aattccgtga aggaggctga
     1981 aatcagctca atccttgagc attccagaat ttcaagaaat gcaggattta catcctgaat
     2041 gatgcaatca ttattatata tcaccattcc cagaggggaa tagttgaaaa tctgttcgta
     2101 tctgttgtca aactgcatta tatggaaggc tgtccgtttt tcacggacag ccgttttttt
     2161 acgagtaaat ctctttgatc agatttgcgt acttgtcagt gattacattt cttttcatct
     2221 tgtaaagatt tgtcatttcg tctccgactt ccatcggttt aggaaggaaa cggaaatcag
     2281 tcaccttttc aaatcccttg aatccgtttt cgcttgaaat cgtatccttg atgatttttg
     2341 aaaaatgagt cactactttt gcattcttgt tcaggtcttc tccaggcttg atctggaacc
     2401 ctgcttccac aagtctttcc aggttaggat aaatcagtgc acccagtgat ttctgatcct
     2461 gaccgacaac cattacattc tgaatcagtg cattctgaag aagaaggttt tctattggga
     2521 ccggttccac gttttcaccg cccagaagaa ccacagtgtc cttgaccctt cctcttacag
     2581 agagagttcc gttgaaactg atgaaaccca gatctcctgt gttcagccat ccgtttttca
     2641 gcactttgtc cgtcgcttcc tgattcttgt aatatccctt catcacctga gggcccttca
     2701 catgaatgat tcccatatga cccacaggga cttcttctcc ctggtcgttc agtattctga
     2761 tctctgtgcc tggaggcgat actcctacgc ttccttgaat gatcttgtcc ttctgacgga
     2821 cagaaataat cggtgagcat tcagtcattc cgtagccctc atatacaggg attccaagta
     2881 cattgaagaa ttcgtccaca tgagcaggaa gagctcctcc acctgagatc gttcctttca
     2941 gttcgccccc gagcgcctct ctgatctttc cgaaaacgac tgaatcaaga atcttgttcg
     3001 gtatgaaaag atttgccgcc gtg
//
LOCUS       DAOQGJ010000251         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19495_length_3018_cov_2.826190, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000251 DAOQGJ010000000
VERSION     DAOQGJ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19495_length_3018_cov_2.826190"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PK683_05200"
     CDS             complement(<1..507)
                     /locus_tag="PK683_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07877.1"
                     /translation="MNIDIRTVTVLSIVSSFFMSISLFAVSKGFLGETGGVRKWAAAM
                     LVQLFSWIFIGALRGYIPEIISVLTGNTLVILSLSMTFSVLAEFEKKSIRKIWMNLLI
                     ILQGAVLAFFTLAVPDISVRIFFSSVCMAVPVSASAYILLKSRSERSPSRTFTGYLYV
                     LFSAISLAR"
     gene            complement(1084..2901)
                     /gene="recJ"
                     /locus_tag="PK683_05205"
     CDS             complement(1084..2901)
                     /gene="recJ"
                     /locus_tag="PK683_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNH07878.1"
                     /translation="MKYEHGQSLSDLKKRPKSFPGFTAFQSYVLESLKLTEEDAEAFV
                     AADVSRLHSPFLLPDIREAVKKIRNFILQKKSILLYGDRDTDGVSSTSLLGIYFREQI
                     RKNGGTLSIRTSTANDDYGLCGTVMTQIQNLKPDLLITLDFGTSNEAEINKLAAQGIE
                     VIVLDHHEIPVRIPRCLLINPKRADSIYPEKRICTSALAAKLIMALLIAEKAEEKTRE
                     STDLLFFSPEKETDYDELNYHDALIKYPDLKKKFEDLLDLAAVGTVTDMMPMLGENRN
                     IVKIGIRQLCELISIEKPSRMGLRSLVRSLGLNKNRITSRDLGWSVGPVLNAAGRMGK
                     TETALKLLLSETEQEAEFLTKEIIGLNQERKERTKRNIFRVDQYFQRKPEREKKRVIF
                     CYEPDMEPGVSGIVATRLVEKYKRPAVFVTPDHGKARGSIRSCNSENVLDVLNMLSEY
                     LEHFGGHPEAGGFSIAINKIPVLEKELEEAAPKWLNGASVDIRVRKSAAEILPTDLDS
                     KLFEEIIALDPFGQSFSIPLLSLKNIRIIHWKIMGDGSHARFSVQNAGSKIKFVIWNS
                     AKELNSLMSEKSEISLWGSLEENYFQGTASLQFQVEHFE"
     gene            complement(2901..>3018)
                     /locus_tag="PK683_05210"
     CDS             complement(2901..>3018)
                     /locus_tag="PK683_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNH07879.1"
                     /translation="VEAKEKVEIGKTGTLIGDIKTPKLHIETGAKFTGHSSM"
BASE COUNT          819 a          655 c          597 g          947 t
ORIGIN      
        1 cctggctagc gaaattgcag aaaatagaac atacagatat cctgtaaatg tacggcttgg
       61 acttctttct gagcggcttt tcagcagaat atatgccgat gccgatacgg gcactgccat
      121 gcagactgag ctgaagaaaa tccggactga gatatccggg actgccagag taaaaaatgc
      181 aagtacagca ccctgcaata taatgagaag attcatccag atttttcgga tgcttttttt
      241 ctcgaattca gcaagaacac tgaaagtcat cgacagtgaa agaattacca gtgtatttcc
      301 tgtcaggaca gatataatct ccggaatata acctcgcaga gctccgataa aaatccagga
      361 aaacaactga acgagcattg ccgctgccca tttcctgacg cctcctgttt ctcccagaaa
      421 tcccttcgat acagcaaaga ggctgatgct cataaaaaat gaactgacga tactgaggac
      481 tgtaaccgtt ctgatatcaa tattcatctt atttcggcca tttctgaaaa gagacgcttt
      541 gacggaactt taggaattat aaagtgcttt attgaattga aattactgct ctgaccagga
      601 attttgcaca ctgaagtctg cctttactgc aattcttgaa actccgatct tttggtcaaa
      661 ctttaaaact ctgtgcagac agattctatt cagctctgac tgatctgcct ttcgtaaaag
      721 aaccgtaatt tatttatatt atatcatttt ttctaaatag agctgttgaa aaaatagttt
      781 caacaactcc atttttaatt tttctaaaaa acctgcagct cgctgaagcg cgcaaacttg
      841 aaaatctgaa actgcctaaa tccctattta gcttctgacg aaagataatt tcttgatttt
      901 ttcaacaagt ctgatgatta tgttttttaa ataaaatcaa tgattttatt gtttctgcaa
      961 tgctgaaatg acgaagataa ttgaaaatga gcacactgtt cactgagagg cagatctgct
     1021 tctttgagaa gcgaagtctt ccaattgaac ctgctttcgg aagaatcaca gcgtcagact
     1081 gatttattca aagtgttcca cttgaaactg aagactagct gttccctgaa aataattttc
     1141 ttcgagactt ccccagaggc tgatttccga tttttcagac atcaggctgt tcagttcctt
     1201 ggcagaattc cagattacaa acttgatttt ggaacctgca ttctgaactg aaaagcgggc
     1261 gtgagatccg tcccccataa ttttccagtg aatgatacgg atatttttta atgataaaag
     1321 aggtatgctg aagctctgtc cgaatggatc gagagcaatt atttcttcaa agagttttga
     1381 atccagatca gtcggaagaa tttcggcggc tgattttctg actcttatgt cgacagatgc
     1441 gccgttcagc cattttggag ctgcttcttc cagttccttt tccagaacag gaatcttatt
     1501 aatggctatg gaaaagccgc ctgcttcagg atgtcctcca aaatgctcca gatattctga
     1561 aagcatattc agtacatcca gcacgttttc ggaattgcag gaacggatgc tccctcttgc
     1621 cttcccgtga tccggagtga caaatactgc aggtcttttg tatttttcca caagtcttgt
     1681 ggccacaatt ccagaaaccc ccggttccat atccggttca tagcagaaga tcactctttt
     1741 cttttctctt tccggctttc tttgaaaata ctgatccact ctgaaaatat ttcttttggt
     1801 tctttccttt ctttcctggt taagtccaat gatttcctta gtgagaaatt ctgcttcctg
     1861 ttctgtctcc gaaagaagaa gcttaagtgc agtttcagtc ttccccattc tgcctgcagc
     1921 attcagtaca ggacctaccg accatcccag atccctcgaa gtaatcctgt ttttattcag
     1981 tccaagactt cttaccagac ttctcagtcc cattctggag ggcttttcaa tgcttataag
     2041 ctcacagagc tggcggattc ctatttttac aatatttctg ttttccccga gcatcggcat
     2101 catatctgtc acagtgccga cggcagcaag atccagaaga tcttcaaact ttttcttaag
     2161 atcagggtat ttaattaatg catcatgata atttaattcg tcataatccg tttccttttc
     2221 aggagaaaag aaaagaaggt cagtggactc cctggttttt tcctctgcct tttctgcaat
     2281 gaggagcgcc ataatcagtt ttgccgcaag tgcggaagtg caaattcgtt tttcaggata
     2341 aatggaatcg gctcttttgg gattgatgag aaggcatctg ggaatacgga caggaatttc
     2401 atggtgatcg aggacaatga cttcaattcc ctgagcggca agcttgttga tctccgcttc
     2461 gttggatgtt ccaaaatcca gggtgattaa aagatccggc ttcagattct gaatttgagt
     2521 cattacagtt ccgcaaagtc cgtagtcgtc atttgcagtg gatgtcctga tggaaagagt
     2581 tccgccgttt ttgcgtatct gttccctgaa atatattcct agaagtgagg tcgaagaaac
     2641 accgtcagta tccctgtccc cgtacaataa aatgcttttt ttctgcagaa tgaaatttct
     2701 gatttttttt actgcctcac ggatatccgg aagcaggaaa ggggagtgca gtctggaaac
     2761 atctgctgcg acaaatgctt ccgcatcttc ttccgtcagc ttcagacttt ccagtacgta
     2821 cgactgaaat gcagtgaacc ccggaaagga ttttggtctt ttttttaagt cggaaagact
     2881 ttggccatgt tcatatttca ttacatggaa gagtgaccgg taaattttgc tccggtttca
     2941 atatgaagtt ttggggtttt tatgtctccg atcagtgtac cagtcttgcc gatttccact
     3001 ttttcctttg cttccacg
//
LOCUS       DAOQGJ010000252         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19509_length_3016_cov_3.712597, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000252 DAOQGJ010000000
VERSION     DAOQGJ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19509_length_3016_cov_3.712597"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..2396
                     /locus_tag="PK683_05215"
     CDS             96..2396
                     /locus_tag="PK683_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07880.1"
                     /translation="MNEIGPKEDRDAFLKLSELFENCLKYNYESDAEALEKFENPLGS
                     DISLYSELSLYSLGAVENKSSYQNKLLSLLKRPEILSDVDQILQCVFLYSVCSSAKEE
                     SLPGLPLPSLEKNWKAHFIHLFRQLQKEENWKYISEIEKLIEQVSNDGFFFHSGLMSA
                     AMMRRLLTAGNSISADGFLRNARICFSNAGSDVLGARLLDEYRERSTLKNSFIYRKYS
                     NPKISQSELEAETAIPEILQILENLPVGIVVFNNRRKLILSNKQCRKLLGLIPVETNL
                     GDFVEKAGFIQSASGFPYRLKNFPVFRSLEGEFISADDMEIYKAGRRVPVQIFSSPIF
                     DSESRVVFAAAAIEDVSERRNTERLLADYNRALETQVSERTNALKAAKEEAEKANHSK
                     AEFLAMMSHEIRTPMNGIIGMARLLQETALSEKQKVYADTLSKSSDNLLTIINDILDF
                     SKIEAGKLELEFVPFNIHLSLKDTVRIFEPKARWQSLKLNYHISPEVPEILLGDETRL
                     RQILFNLLGNALKFTSRGEVSLNVNLYSAEAGEYRLLFEVKDSGIGITDEKMEKLFQP
                     FTQADSSTSRKYGGTGLGLVISKKLAELMGGTIWAYSRFGEGSSFYFTVRMRSTFVLQ
                     NTEETEESIKGLPEDLSILLAEDNEINQIVAENIFETLGYRIDIASNGVEAVQMSLNT
                     FYHVIFMDIHMPEMDGIEAAKLIRERKKSLHCPVIIALTANAMHGDREKYMREGMDDY
                     ISKPFSKSDLIGKISLWKERFPSPSI"
     gene            complement(2380..3015)
                     /locus_tag="PK683_05220"
     CDS             complement(2380..3015)
                     /locus_tag="PK683_05220"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNH07881.1"
                     /translation="MGTSQPAISRALKELEKEASVCQKCKLAATRTMVVFGEGNPDAD
                     LMFIGEGPGVTEDQTGRPFVGKAGNLLTRLIEKGMGLSRSDVYIANIVKCRPTVDQKM
                     QKDRPPDKEETDACSPYLMKQIEIISPKVIVTLGNPSTKFLLKTAQGITALRGKWQEF
                     NGIAVMPTYHPSYVLRNGGETSAVQKDIWSDLQEVLKKLGMTPKVKIKWKD"
BASE COUNT          879 a          656 c          668 g          813 t
ORIGIN      
        1 cggcaaaatc tgtcggcaga cacaatggaa ggaactgaat gaactttgga gaaattctgg
       61 actgtatttg gaaccctctg tctctaaaac agaaattgaa tgagatcggg ccaaaagaag
      121 acagggatgc cttcctgaaa ctgtcggagc tttttgagaa ctgcctcaaa tacaattatg
      181 aatctgacgc agaagctctg gaaaaattcg aaaatcctct gggatcggat atcagtcttt
      241 attcagaact cagtttatac agcctcggtg ccgtcgaaaa taagagttct tatcaaaaca
      301 aacttctaag ccttctgaaa aggccggaaa ttctgtctga tgtggaccag attctgcagt
      361 gtgtctttct ctattcagtc tgcagttcag ccaaggaaga atccttgcct ggtctccccc
      421 tgccctcact ggaaaagaac tggaaagctc attttattca tctgttccgg cagctccaga
      481 aagaggagaa ttggaaatat atttcagaaa ttgaaaagct gattgaacag gtatccaatg
      541 acggattttt ctttcattcc ggtctgatgt ccgctgctat gatgcgcaga cttctcactg
      601 ccggcaacag tatttctgcg gacggttttt taagaaatgc caggatctgc ttttcgaacg
      661 caggctcaga tgttctgggt gcaagacttc tggatgaata cagggaacgg tctactctca
      721 aaaattcgtt catatacaga aaatattcca atcccaaaat cagtcagtcc gaactggaag
      781 cggagactgc catccctgaa atcctgcaga ttctggaaaa tcttccggta ggaatcgttg
      841 tattcaacaa caggagaaaa ctgatcctgt caaacaaaca gtgcaggaaa ctgcttggcc
      901 tcattccggt cgaaacaaac ctgggagact ttgttgaaaa ggcaggattc attcagtctg
      961 cctccggttt tccctaccga ttgaaaaatt ttccagtgtt cagatcgctt gagggcgaat
     1021 ttatcagtgc agatgatatg gaaatttata aagcgggaag aagagtacct gttcagattt
     1081 tttccagtcc catttttgac tcagagtcca gagtagtctt tgcagccgca gccattgaag
     1141 atgtttcaga aagaagaaat acggaaagac tgcttgcaga ttacaacagg gctttggaga
     1201 ctcaggtatc cgaaagaaca aatgcactga aggcggcaaa ggaagaagct gaaaaggcga
     1261 accattcgaa ggctgaattc ctggcaatga tgagccatga gatcagaact ccaatgaacg
     1321 ggatcatcgg catggcaaga cttcttcagg aaacagctct ttcagaaaaa cagaaagttt
     1381 atgcagacac tctttccaaa agcagcgaca atcttcttac aatcatcaat gatatattag
     1441 atttttctaa aatagaagcc ggcaagctgg aactggaatt tgttccattc aatatccatc
     1501 tttctctcaa ggatactgtg cgtatttttg aacctaaggc aagatggcag agcctgaaac
     1561 tgaattatca tatcagtcct gaggtgccag aaattctact gggcgatgag accaggcttc
     1621 ggcagattct cttcaatctt ttaggcaacg cattgaaatt cacttccagg ggagaggtaa
     1681 gtctgaatgt aaatctgtac tccgctgaag caggagaata cagacttctt tttgaagtga
     1741 aagattccgg gattggaatc accgatgaga agatggaaaa actttttcag cccttcactc
     1801 aggcagactc ttccacttcc agaaaatacg gaggaacagg tcttggactg gtcatctcca
     1861 aaaaacttgc ggaactgatg ggcggcacaa tctgggcata cagcagattc ggggaaggaa
     1921 gttcctttta ctttacagtc agaatgagaa gcacttttgt tctgcagaat acagaagaga
     1981 ctgaagagtc catcaaaggc cttcctgaag atctctccat tcttcttgct gaggacaatg
     2041 aaatcaatca gattgtggct gagaatatat tcgaaaccct gggatataga attgacatcg
     2101 cttcaaacgg agtggaggca gtgcagatgt cgctcaacac attttatcat gtaattttca
     2161 tggatattca tatgccggaa atggacggaa tagaagcggc aaaactgata cgtgaaagaa
     2221 aaaaatcact gcactgccct gtcattattg cattgacagc aaatgcaatg catggggaca
     2281 gggagaagta tatgagagag ggaatggatg attatatcag caaacctttc tccaagtcag
     2341 atctgatcgg aaagatcagc ctttggaagg aaagatttcc tagtccttcc atttaatctt
     2401 gactttagga gtcataccaa gtttcttcag tacctcctga agatcactcc agatatcttt
     2461 ctgaaccgca ctggtctctc ctccgtttct caaaacataa ctggggtgat atgtaggcat
     2521 tactgcaatt ccgttaaact cctgccattt gcccctgaga gctgtgattc cctgtgctgt
     2581 cttcaataga aattttgtgg acggatttcc aagtgtgaca atgacttttg gagatatgat
     2641 ttctatctgc ttcatcagat aaggactgca ggcatctgtc tcttccttgt ctggaggcct
     2701 gtccttctgc attttctgat ccacagtggg tctgcatttt acgatattcg caatatacac
     2761 atccgaacgt gaaagtccca ttcccttttc aatgagtctg gtcagaagat ttccagcctt
     2821 gcctacaaac ggtcttccag tctggtcctc ggtcactcca ggaccttctc cgataaacat
     2881 cagatcagca tcaggatttc cttctccgaa cacaaccatt gttcttgttg cggccagctt
     2941 gcatttctga cagacggatg cttccttttc cagttccttc agcgcacggg aaatggcggg
     3001 ctgcgaagta cccagc
//
LOCUS       DAOQGJ010000253         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19539_length_3013_cov_3.809331, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000253 DAOQGJ010000000
VERSION     DAOQGJ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19539_length_3013_cov_3.809331"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..2348)
                     /locus_tag="PK683_05225"
     CDS             complement(126..2348)
                     /locus_tag="PK683_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNH07882.1"
                     /translation="MHYRLVFWRGLNKFYLEDLSAGVSVFLIAVPLCLGIAHASGAPV
                     LSGIISGIIGGIIAGFLSGSRLSVSGPAAGLTSVLIASLHSLGSMEALLSAVVVAGLF
                     QLLLAFLKAGRLADFFPAAVVSGMLAAIGILLIIKQVPHAIGYDVVEFGVEEFSLNKE
                     DIHETYDDPLAKQEKNTLTVLLHSFTHIKTGIALIGFASLAVFILWEKYASRIIRWMP
                     ASLAAVLTGIMISEIFDIYFPLMHPGKDNMVQLPVISSVSDIGKAQLFPAFPAFMNPE
                     VWKVGFTLAMIASLETLLSVEAIDRIDPAYETTPKNRELFAQGVGNIFCGLLGGIPVT
                     SVIVRSSVNLEAGAKSKNSAVIHGFLLFITSLFLAPLINRIPLASLAVILVIIGYKLS
                     SLKLFSKMYRLGLGQFIPFTVTVGAILFTDLLTGVFVGFLASLIVITRNHYELIQITV
                     KDMGRTKKITLLQYTTFLHKAGLKKILHEIPKGSIVEVDGSGTRYIDQDVISLLSEFR
                     EFSGKSGTELIVSGIPSIPKYSKEFENRMNLNLQKMLENNIKWAEEMLKTDPGFFKNL
                     SQGQSPDFFFIGCSDSRITVNEMTGTSAGDIFVHRNIANIVTGSDMNLMSALEYAVDV
                     LKVQHVIVCGHYGCGGVKAAIDEKSNPGNTISNWIQGIVKEYEANRNELDSISDKQVK
                     ENRMIEYHLMAQMKNLSKLNVIRNAVEKFGFPKLHAWIYDIHTGHVKVIAENYNEERV
                     "
     gene            complement(2364..>3013)
                     /locus_tag="PK683_05230"
     CDS             complement(2364..>3013)
                     /locus_tag="PK683_05230"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH07883.1"
                     /translation="LNFINSYLKIMSPIIHDHSGFIDKYIGDAIMALFPNETDDAILS
                     AVSMLTALRGYNRNRNSSGYRPIDIGIGINTGNLMIGTIGGQNRMEGTVISDAVNIAS
                     RVESMTKIYGANLLITENCFQSIKNPSPFLIRKIDQVVAKGKKSAVWIYEVFNADSEE
                     MKDQKLKTMNLFSEAVSEYSEKKYKNAYNLFSQVKEKAHLDRITEYFLKTLNSTV"
BASE COUNT          852 a          686 c          607 g          868 t
ORIGIN      
        1 gggactgccg gcaaacttgc tctgccagag ttccgttttt ccttttttct ttgacttaat
       61 tttgatatag ttcacatata atgaactgat aatatcattg tatttttcca aaagtttttt
      121 cctgctcata ctctctcctc attatagttt tcagcaataa ccttcacatg accggtatga
      181 atatcataaa tccaggcatg aagtttcgga aaaccgaatt tctcgactgc atttctgatg
      241 acattcagct tggaaagatt tttcatctgt gccatcagat ggtattcaat catcctgttt
      301 tccttcacct gtttgtccga aatactgtcc agctcgtttc tgttagcctc gtactccttc
      361 acgatcccct gtatccagtt ggagattgta ttgcccggat tggacttttc atctatggct
      421 gccttgactc ccccgcatcc gtaatgaccg catactatca catgctgtac tttcagcaca
      481 tccactgcat attcaagggc cgacatcaga ttcatatccg aacctgtcac aatgtttgca
      541 atattcctgt gaacaaaaat atctcctgcg gaagttccgg tcatttcatt gactgtgatt
      601 ctgctgtctg aacatcctat gaaaaagaaa tccggagact gtccctgaga aaggttttta
      661 aaaaatcccg gatcagtttt cagcatttct tccgcccatt ttatattgtt ctcaagcatt
      721 ttctgcagat tcagattcat cctgttttca aactcctttg aatattttgg gatactcggt
      781 atccctgaaa cgatcagttc agttccggat ttcccggaga actctctgaa ttctgaaaga
      841 agagaaatca catcctgatc aatgtatctt gttccgctcc cgtccacttc aacgatcgaa
      901 cctttcggaa tctcgtgcag aatttttttg agtcctgcct tgtgaagaaa tgtagtatac
      961 tgaagcagtg taatcttctt ggttctgccc atatctttca cagtgatctg gatcagctcg
     1021 taatggtttc ttgtaattac gatgagtgat gcgagaaatc cgacaaatac tccagtgagc
     1081 agatcagtaa acagaatcgc acctacggtg acagtgaacg gaataaactg tccaaggccg
     1141 agtctgtaca tcttgctgaa gagcttcagg ctcgaaagct tgtaaccgat gattacaaga
     1201 atcacagcaa gagacgcgag aggaattctg ttgataagag gggccagaaa aagagatgtg
     1261 atgaagagaa gaaatccgtg aatgactgcc gaattttttg acttggctcc tgcctccaga
     1321 ttgacggaac ttctgacaat cactgaagtg actggtatcc cgcctaggag cccgcagaaa
     1381 atgttcccta ctccctgcgc aaaaagttcc ctgttcttgg gagtggtttc ataggcaggg
     1441 tcaatcctgt ctattgcctc cacggaaaga agggtttcga gagatgcaat cattgcaaga
     1501 gtaaatccga ccttccatac ttccggattc ataaatgcag ggaatgcagg aaaaagctga
     1561 gctttcccaa tatcagaaac actgctgatg acaggaagct gaaccatatt gtccttaccg
     1621 ggatgcatta atggaaaata tatatcaaat atctctgaaa tcatgattcc agtaagcacg
     1681 gctgcaagag aggcaggcat ccatcggatg atacgggacg catatttttc ccagagaata
     1741 aatactgcaa gtgaagcaaa gccgatgagt gcaatgccgg tcttgatatg ggtaaaagaa
     1801 tggaggagaa cggtcagagt atttttctcc tgttttgcaa gaggatcatc ataagtttca
     1861 tgaatgtcct ccttgttcaa agaaaactct tcaactccga attccactac atcgtacccg
     1921 attgcatgag ggacctgttt aataatcaga agtattccga ttgcagcaag cataccgctg
     1981 accactgccg caggaaaaaa atcggcaagc ctgcccgctt tcagaaatgc gagcagtaat
     2041 tgaaaaagac ctgcaactac aactgcagaa agaagcgctt ccatactccc caatgaatgc
     2101 agagaagcaa taagcacact ggtgagtcct gcggcaggtc cactgacaga aagacgcgaa
     2161 ccgcttaaaa aaccggcaat aattcctccg atgattccgg aaatgattcc ggaaagcaca
     2221 ggtgcaccgg aggcatgtgc aatacctagg cataaaggta ctgcaataag gaatacactg
     2281 acaccggcac ttaagtcttc gagataaaac ttatttaatc cgcgccaaaa cacgagacga
     2341 taatgcaatt cttcaggatt ttgtcaaacc gtagaattga gcgtcttcag aaaatattcg
     2401 gtgattctgt ccaggtgtgc cttctccttt acttgactga atagattata tgcattttta
     2461 tattttttct cagagtattc ggacactgct tctgaaaaaa gattcattgt cttcagtttc
     2521 tgatccttca tttcctccga atccgcattg aatacttcat aaatccatac tgcagatttt
     2581 ttccccttgg caacaacctg atcaattttc cgaatcagaa aaggagatgg attttttatg
     2641 ctttgaaaac aattttccgt gatcagaaga ttcgcaccgt agatcttggt catactttcc
     2701 accctggatg cgatattcac tgcatcactg atgacagttc cttccatcct gttctgtcct
     2761 ccgatcgtac ctatcatcag gtttcccgta ttgattccta tccctatgtc aatcggtctg
     2821 taaccggaag agtttctgtt ccggttgtat cccctcagag ctgtgagcat ggaaacagcc
     2881 gagagaatcg cgtcatcggt ctcattcgga aacagtgcca tgatcgcatc tccgatgtac
     2941 ttgtcaataa aaccgctgtg atcatgaatg atgggagaca ttattttgag atatgaatta
     3001 atgaaattaa gag
//
LOCUS       DAOQGJ010000254         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19544_length_3013_cov_3.120690, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000254 DAOQGJ010000000
VERSION     DAOQGJ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19544_length_3013_cov_3.120690"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1198)
                     /gene="dxr"
                     /locus_tag="PK683_05235"
                     /pseudo
     CDS             complement(1..1198)
                     /gene="dxr"
                     /locus_tag="PK683_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776147.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
     gene            complement(1195..1503)
                     /locus_tag="PK683_05240"
     CDS             complement(1195..1503)
                     /locus_tag="PK683_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07884.1"
                     /translation="MKVLYFLMMILAVNCMSSVHHFNQSEPMTFQEYSSAKKISADSE
                     QFVILWFASDTNYVEEAVDALNRQCPDGKVDQIGTTFKTKLGFLSWTNQLHLVGYCRK
                     "
     gene            complement(1500..1817)
                     /locus_tag="PK683_05245"
     CDS             complement(1500..1817)
                     /locus_tag="PK683_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07885.1"
                     /translation="MSKKIKFTIIIGMILFSAIGCVQLQSVTINSQPSPEERKTKISA
                     ESDKLVFLAFNFNNDFLEETPKKLLDQCKDGKIRGIISKFETVHYFIFIRYVVKASGY
                     CTK"
     gene            complement(1810..2244)
                     /locus_tag="PK683_05250"
     CDS             complement(1810..2244)
                     /locus_tag="PK683_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07886.1"
                     /translation="MIMRFTYLLIVFLIHCAFPHHFTMGDLDGTSGKRKHFQVLVSET
                     GFSFEEGLTIAQAGLLAANRNANMNTARDIQYVKTIIALSTMGPVTGRKTFNNTYADE
                     LADIVYQHCKSGKIVNLKNIRESARYPVVSGEIVRIEGDCLE"
     gene            2385..>3013
                     /locus_tag="PK683_05255"
     CDS             2385..>3013
                     /locus_tag="PK683_05255"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07887.1"
                     /translation="MIIPYGDANFESVVTKKALLIDKTKYIPLLEKHSKFFCIRPRRF
                     GKSFMLSLLQSYYDIKTKDKFDRLFGGLAIHKNPTDLRNTYIILKMDFSGLNADTDEI
                     LKKEFTNRIKYDAGFVLRYYSEVFSEKVYDEYRSTILEKDEEPAAAVKFLLQYVKETP
                     YKVYVLIDEYDNFINNLVSLDREGFARRILSRSGFVRSFYETLKIGSGEG"
BASE COUNT          836 a          665 c          661 g          851 t
ORIGIN      
        1 agtgaatctg cttccagata tccctgcaga ccgtccggat aagagaccgg gattttttcg
       61 agcgcatatt cgatgagtct tgtaatttcc ctgaatcctg cctttttgga aaggaaaagc
      121 tccacagcgg cctcatttgc agcattgaat actgctggag ccgttccgcc gattcttccg
      181 gctgcatagg ccagtttcac ggctggatac ttctgatgat ccacatccct gaactgaagt
      241 atccagccgt gaaactggcc tatgcagccg gaagaatcgg cggaacggca ggatacttct
      301 gatgatccac atccctgaac tgaagattcc ggaaactgtg cggctttctt tcgatgagaa
      361 gggatgggac aggggccgga taaaacattg catgagccgc cggaaagacc atatccggat
      421 gagaggaaag catctgtgtg gaaccgtcat tcagttcgat gattccgtga acgatgcttt
      481 ccggatgaat cacaatttcg atcctgtcgt acggtatgtc aaaaagataa tgtgcttcaa
      541 tgacctccag agacttgttc acaagacctg cagaatccac agtgatcttc ggacccatct
      601 tccaggtagg atggttcagt gcttcctcca ctgtgacagt ttccagtctt tccgcaggcc
      661 agtctctgaa cgaacctccc gaagcagtga gggtcagtgc acggatatcc gatttcgact
      721 tgtgttcgag aagctgaaaa agcgcattgt gctccgagtc cactggaatg atctgaatcg
      781 gattttcccg gagaagtttt ttaatgtaag gaccgaatgt gaccaatgtt tccttgtttg
      841 caattgcgag tttctttcct gccttgatgg ccgcaattgt aggcatcaca cccactgcac
      901 ccactacggc agtcaccaca gtgtccactg ctttttcgga aacaatctca gaagaaaact
      961 catttccata aagaatctta gtatctgcat atttatttcc gagcacagac ctgtctgcat
     1021 cctctgcaga aatgacagct gtttccggat gaaactcgtt gatgatcgac tttgccttgt
     1081 ccagattctt atgcacctga aaggaatgaa gacggaaatg attcggaaac ctccgaagaa
     1141 ccttgattac agaatcaccg acggagcctg aggctcccag aagagagatg gaattcattt
     1201 ccggcagtag ccgaccagat gcagctgatt tgtccaggaa agaaaaccca gcttggtttt
     1261 gaaggttgtt ccgatttgat ccacttttcc gtcaggacac tgcctgttca gtgcatccac
     1321 tgcctcttcc acataatttg tatcactcgc aaaccataag attacgaact gttctgagtc
     1381 tgcactgatt ttttttgctg agctgtattc ctgaaatgtc atcggttcag actgattgaa
     1441 atgatgcacg gaagacatac agtttacagc cagaatcatc attaagaaat ataggacttt
     1501 cattttgtgc agtatccgga tgctttcacg acatatctga tgaatataaa ataatgaaca
     1561 gtctcaaact tggaaatgat tcctcggatc tttccgtcct tgcactgatc cagaagtttt
     1621 ttgggagtct cttccagaaa atcgttgttg aagttgaatg caaggaaaac cagtttgtcg
     1681 gattctgctg aaatctttgt ctttctttct tcaggagaag gctgggagtt gatcgtgaca
     1741 gactgaagct gaacacatcc gattgcgctg aaaagaatca ttcctataat gatagtaaat
     1801 tttatttttt tactcaagac agtctccttc aattctgacg atttctccgc tcactacagg
     1861 atatcttgca gattccctga tatttttcag attcacaatt ttaccggact tgcagtgctg
     1921 atatacaatg tcggccagtt catcagcata tgtattgttg aatgtctttc ttccggtcac
     1981 aggtcccatt gtggacagag cgataatggt ctttacatac tggatgtctc tggctgtgtt
     2041 catatttgca tttctgtttg ctgcaagcag tccagcctgt gcaattgtaa gaccttcttc
     2101 aaaactgaaa ccggtctcac tgaccagaac ctgaaaatgc ttccttttgc cggaagttcc
     2161 gtccagatcc cccatagtga aatgatgagg aaatgcgcag tggatcagaa aaacgatgag
     2221 cagataggta aatcgcataa tcactgatta ttcagaaaat aaagatgagt caatcaaaaa
     2281 ttgagaacag tctgaatttc attaacggat ctgtgttcct tgcgggttcg ggccagcaca
     2341 tcctttctgc ttgacacctg acttacaccg ttcagtttag aattatgatc attccatacg
     2401 gcgatgcaaa ctttgagagt gtggttacaa aaaaagccct cttaattgat aagacaaaat
     2461 acattcctct tttggaaaaa cacagcaaat tcttctgcat ccgtccgcgc cggttcggga
     2521 aaagttttat gctgagcctt ctgcagagtt actatgatat caaaaccaag gacaaatttg
     2581 acaggctctt tggtgggctc gcgattcata aaaatcccac cgatctgaga aacacataca
     2641 tcatcctgaa gatggatttc tccggactca atgcggatac agatgagata ctgaagaagg
     2701 agtttacgaa cagaattaaa tacgatgcag ggtttgttct tcggtattac tctgaagttt
     2761 tcagcgaaaa agtttatgat gaataccgtt caacaatact ggaaaaagat gaggagcctg
     2821 ccgccgccgt aaaattcctt ctgcagtatg tcaaagaaac accgtataaa gtctatgtgc
     2881 tcattgatga atatgataat tttataaaca atctggtttc actggacaga gaaggttttg
     2941 caagacggat tctttcccga agcgggttcg tgagaagttt ttatgaaaca cttaagatag
     3001 gaagcggcga agg
//
LOCUS       DAOQGJ010000255         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19598_length_3008_cov_3.849983, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000255 DAOQGJ010000000
VERSION     DAOQGJ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19598_length_3008_cov_3.849983"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3008
                     /locus_tag="PK683_05260"
     CDS             <1..>3008
                     /locus_tag="PK683_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07888.1"
                     /translation="QYEISKLYRERSSAFAKLAFDSLYLFYKDDPLYPIYKSRADWQM
                     IKEYTSEGYMEEADRLVQDMLKTKHNPNYGFSYALAKSYLDRKKNLNTSQEILNYCEE
                     MRHQKNLHPEMIPSLYYWAAEVHMMFNENDKAIDLFKRVLSEYPGFYRKTEMKRMIGE
                     LEFRKLKDHIPAYYQEVFGTDVSNELHDKKIADDVLSFFENIGGYKKRIRIAEEYLNG
                     GINNKEILFLLKYVQAYSLFKLDRFEESLQKLDEYLSDTRKGSYVGLLSRLLKFENAK
                     NLGNTDLSNQELNEFILNYSRKAGVKVSSADIKDSFFYFETKARSADFSQKTDEAIRF
                     YSFNNKLLMNASISRFPMESFADRFSPFYHKKMTDTVINAARKEQQEKEKSLLTKLNL
                     LGKERLDAYGRSTSLAAYFFRWSPFRVFGDFRDIRPAAFFSTDRLRSAEKFYEENLKT
                     ARENLDAGTVYGFSYFLIMQSVMNESHYLNENSLTYARKKEILKNLKQAEYELRWLIY
                     ADPDYTEAYLLLGWLYQYIEVRKNTVVFPENEEDSRVYESLYARYFPLKKYLEEAVEL
                     YTQIEEFLGERASRKVKSDLHLNMGNCQFQLKNQKNALGHYEEVEKLSRHIQERTRFE
                     NYQQNAVFYFNFARAKIFNAELESARESFQKSMQLYYKYEYYPLVSKIGTDQNAQKEN
                     IESLNEVKKKLALLHTLTGITEIELEKYDEAILSLSTAVSMNGDSDYINDMNLYNALA
                     ICYQQTGDYERSEIALRLSDREFQKKKRITEIIDFSFWDLVIPDQTRIQGEGRFPEGM
                     PLAFTNLITRGIRIQNLADKKEFFMAAEKIQERSEFILDNKLDRVSIADRILKGTVSE
                     LAYNEYQRGNYFQAADFYLKDKELKISSGKDYEAYKAFIRERISIFSFVEENTEKISL
                     LEERLRSGISQLNVFHEKEIRECLPVFSGNDSDRLKCEKDFRKNFPEFDIYLGYTYFY
                     LGEIISRTDNKEAAYSWYGNALLLLKNPAG"
BASE COUNT          995 a          536 c          660 g          817 t
ORIGIN      
        1 cagtacgaaa tatccaaact ttacagggag aggtcttctg cttttgcgaa acttgccttt
       61 gattcccttt atcttttcta caaggatgat cctctttatc ctatatacaa gtcccgtgca
      121 gactggcaga tgatcaagga gtatacttca gaagggtata tggaagaagc agacaggctt
      181 gttcaggata tgctgaagac gaagcataat cctaattacg gattttccta tgcactggca
      241 aagtcttatc tggacagaaa aaaaaatctg aacacatcgc aggagattct gaattactgc
      301 gaggaaatga gacatcagaa gaatctccat cctgaaatga ttccctcttt gtattactgg
      361 gctgcagaag ttcatatgat gttcaatgaa aatgataagg caattgatct tttcaaaaga
      421 gttctttccg agtatcctgg cttttacaga aagactgaaa tgaaaaggat gatcggagag
      481 cttgaattca ggaaactgaa agatcatatt cctgcatatt atcaggaagt tttcggaaca
      541 gatgtttcaa acgaactgca tgacaagaag attgcggacg atgtcctgag tttttttgaa
      601 aacatcggag gctataaaaa gcggataaga attgctgaag aatatttaaa cggaggaata
      661 aacaataagg aaatactgtt tcttctgaaa tatgtgcagg cgtattctct tttcaagctg
      721 gaccgatttg aagagagtct gcagaagctt gatgaatacc tttcagatac aaggaaggga
      781 agctacgtag gacttctttc gagactcctg aagtttgaaa acgcaaagaa cctgggaaat
      841 acagacttgt ccaatcagga actcaacgag ttcattctga actattccag gaaggccggg
      901 gtaaaagtaa gttctgcaga tattaaggat tcattttttt attttgagac taaggccaga
      961 agtgcagatt tctctcagaa gacagatgaa gcgatccgtt tctattcctt caataacaaa
     1021 cttttgatga atgcaagcat ttccaggttt ccaatggaaa gttttgcgga cagattttct
     1081 cccttttacc ataaaaaaat gacggatact gtcatcaatg cagcaagaaa ggaacagcag
     1141 gaaaaggaaa aatctctcct gacgaaactg aatctgctgg ggaaagaaag actggacgca
     1201 tacggaagaa gcacttcatt ggctgcatat tttttcagat ggagtccttt cagagtgttc
     1261 ggagactttc gggatataag gcctgcggca tttttcagca ctgacaggct cagatccgcc
     1321 gaaaagtttt atgaagaaaa tctcaagact gcgagagaga atctggatgc gggcaccgtt
     1381 tacggatttt cctatttttt aattatgcag tcagtaatga atgaaagcca ttatctgaat
     1441 gaaaactcac tgacatatgc cagaaaaaag gaaattctga aaaacctgaa gcaggcagag
     1501 tatgaactga gatggctgat atatgctgat cctgattata cagaagcata tcttcttctg
     1561 ggatggcttt atcagtatat agaagtgagg aagaacactg ttgtattccc ggaaaatgag
     1621 gaagattcca gggtctatga atctctgtat gcaaggtact ttcctctgaa aaaatatctg
     1681 gaagaggcag tcgaacttta tacccagatt gaggaatttc ttggcgaaag agcatccaga
     1741 aaagtcaaat cggatctcca tctgaatatg ggcaactgtc agtttcagct caaaaatcag
     1801 aaaaatgctc tcgggcatta cgaagaagtg gaaaaactct ccagacatat tcaggagagg
     1861 acaagatttg aaaactatca gcagaatgct gtgttctatt ttaattttgc aagggccaaa
     1921 atcttcaatg cggaactgga atcggccaga gaatcctttc aaaaatcaat gcagctttat
     1981 tataagtatg aatactaccc tcttgtttcc aaaataggaa cggatcagaa tgcacagaag
     2041 gaaaatatag agagcctgaa tgaggtgaag aagaaactgg cactgcttca cactcttacg
     2101 ggcattaccg agattgaact ggaaaaatat gacgaggcca ttctttctct ttccacagct
     2161 gtttcgatga acggagattc tgattacatc aatgatatga atctttataa tgctctggca
     2221 atctgttatc agcagacagg tgactatgaa agatcggaaa ttgcccttcg tctttcagac
     2281 agggagtttc agaaaaaaaa gagaattact gagatcattg atttttcttt ctgggatctg
     2341 gtgattcctg atcagacaag gatacagggg gaagggcgtt ttccggaggg aatgcctctg
     2401 gcctttacga atctgattac acggggaatt cgaattcaga atcttgccga caaaaaagag
     2461 ttctttatgg ctgccgaaaa aatacaggaa agatctgaat tcattttgga caacaaactg
     2521 gatcgggttt ccatcgcaga cagaatactg aaaggcacag tatctgaact ggcatataat
     2581 gaatatcaga gaggaaatta ttttcaggct gccgattttt atcttaagga caaagaactg
     2641 aaaatttctt ccggaaagga ttatgaggct tacaaagcct ttatcagaga acggatttcc
     2701 atattttctt tcgttgaaga aaatactgag aaaatttctc tgcttgaaga aagactccga
     2761 agcggaatca gtcagctgaa cgttttccat gaaaaagaaa taagggaatg cctgcctgtt
     2821 ttttcaggaa acgattcaga ccgcctcaaa tgtgaaaaag atttcagaaa gaattttcct
     2881 gaatttgaca tttatctggg atatacctat ttttatcttg gagagataat cagcagaact
     2941 gacaataaag aagcagcata ttcctggtac gggaacgcac tgcttctcct gaaaaatccg
     3001 gcaggcat
//
LOCUS       DAOQGJ010000256         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19604_length_3008_cov_2.898408, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000256 DAOQGJ010000000
VERSION     DAOQGJ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19604_length_3008_cov_2.898408"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PK683_05265"
     CDS             complement(<1..700)
                     /locus_tag="PK683_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH07889.1"
                     /translation="MPFGCEFNLACVKDVALYQLFMNFIRYYPIAGAAFVIFYIWKKD
                     YFARFRIQEKYPKTEKILHELRQSALTLVIFAAIGTTVYVLKRMNILDNKIYRDPSLY
                     GGVPYMILSYILLTVWHETWFYWAHRLMHHKKIYKYVHLTHHQSVNPTPIAAYHFHWR
                     EAILEGIYPVIFFMFVPMYFPVFVIHTFHVMILNIWWHLGYELFPKGFASHPILKWIN
                     TSTHHNMHHQKFNGN"
     gene            complement(792..1907)
                     /locus_tag="PK683_05270"
     CDS             complement(792..1907)
                     /locus_tag="PK683_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH07890.1"
                     /translation="MNPDLQINIFNSFIAFSSALSFLFFLGEIFSVKSNSRNFYLACV
                     FLFLSLFLNQAFLWSSRLIVFYPHLLHMHMPATVLIGVFLERYLVLMWENQAEPVSKF
                     IYKSAACLLPVLFIVPFFLLSETEKLDYIRTAVKEGAPLRTKLAVIFVILIQFIFFFR
                     LQRRFMKLFRSSTIYSSVTLRLILCILVIGFLCIAVSSAYALNGKITGLEANAYIIGV
                     FLIFLYIVRMRNPEIFIEVRKIVEEEKKYKNSQLKSIDLSKVGEKLKNLMEKEKVYRE
                     ETLSLPELASMIGISPHQLSEYLNQEMKTSFFQLVHRYRIMEAKEKLISNPKETILSI
                     AYSAGYQSKSTFNEIFKKETGLTPTEFRKKSKKSPNL"
     gene            complement(1952..2347)
                     /locus_tag="PK683_05275"
     CDS             complement(1952..2347)
                     /locus_tag="PK683_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNH07891.1"
                     /translation="MELTLSTPALLFPAISLLLLAYTNRFLALTKLIRDLHDEYLESR
                     EEVILRQIRSLRQRLTIIRNMQACGVLAFLLCVVCMFFLFNENKRLAEILFQTSLLLM
                     MFSLLFSIWEIQISTKALSLELQDIEDRK"
     gene            2493..>3008
                     /locus_tag="PK683_05280"
     CDS             2493..>3008
                     /locus_tag="PK683_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07892.1"
                     /translation="MKIIFGFVLISFSLYAEKFTIKDEADYHVHLNHNYEYEMGGVRT
                     KFADWVPYKLHKWNPKFLEDFYELYGLRLHYNENDLRKDIYFLKIGLKSRFRHPRNAL
                     CKITDEETYYKYRLLLTMHLNLQIMRSYMRIASQYDKRHLYFYNLDFAHELKNSFTKS
                     KEIYKEALIYWN"
BASE COUNT          920 a          582 c          646 g          860 t
ORIGIN      
        1 agttcccgtt gaatttctga tgatgcatat tatggtgagt ggaagtgttt atccatttga
       61 gtataggatg acttgcaaat cctttgggaa aaagttcata acccagatgc caccagatgt
      121 tcaggatcat cacatggaaa gtatggatca caaaaacagg aaaatacatc ggaacaaaca
      181 taaaaaatat tacagggtag attccttcga gaattgcctc tctccagtga aaatgataag
      241 ctgcgatagg agtagggttt actgactgat gatgagtcag atgcacatat ttgtaaattt
      301 ttttatgatg catcagtctg tgcgcccagt agaaccatgt ttcatgccag acagtcagca
      361 gaatatagga taagatcata tacggaacac ctccgtaaag agaagggtct ctgtagatct
      421 tattgtccag gatgttcatt cttttcagca catacactgt tgtcccgatt gctgcaaaaa
      481 tcacaagagt cagtgcagac tgtctgagtt catgaagaat cttttcagtc ttaggatact
      541 tttcctgaat tctgaatctg gcaaaatagt cttttttcca gatataaaaa atgacaaagg
      601 ctgctcctgc aataggatag tacctgatga agttcataaa aagctgatac agtgccacgt
      661 ccttgacgca ggccagattg aattcacatc cgaaaggcat gatgaaatcc tccttgtttt
      721 tctcattctc gcacagttct gaaaacggtt cgaccgcttc gatcggaaca gacgaatttt
      781 tcggttcgat attataagtt cggacttttt ttggattttt ttctgaattc tgtgggagtg
      841 aggccggttt cttttttgaa aatttcattg aatgtggatt tggactgata ccctgcactg
      901 taagcgatgg aaaggatggt ctccttcggg tttgagatca gtttttcctt tgcctccatg
      961 atcctgtacc tgtggacgag ctggaagaaa gatgttttca tttcctgatt gagatattct
     1021 gaaagctgat gaggactgat tcctatcatc gaagccagtt caggaaggct caaggtttct
     1081 tctctgtaga ctttttcctt ttccatcaga tttttcagtt tctctcctac cttggaaagg
     1141 tcaatggact tgagctgaga atttttgtac ttcttttcct cttcaactat ctttctcacc
     1201 tcaatgaata tttcgggatt cctcattctt acaatataaa gaaaaatcag aaacacccca
     1261 atgatgtatg cgtttgcctc aaggcctgtg atttttccgt tcagtgcata cgcagaactc
     1321 acagcgatgc ataaaaaacc gatgaccaga atgcagagga tgagccggag agtcacggat
     1381 gaataaatcg tggagcttct gaacagtttc ataaaacgtc tctgcagtct gaagaagaat
     1441 atgaactgaa tcagtattac aaatatcaca gcgagttttg tgcggagagg agcaccttcc
     1501 ttgacagcag ttctgatata gtccagcttt tcggtttcgg aaagaaggaa gaaaggaacg
     1561 atgaagagca ccggaagaag acaggctgct gatttataaa taaatttact tacgggttct
     1621 gcctgatttt cccacatcag cacaaggtat ctttcgagaa atactccgat gagcacagtg
     1681 gcaggcatat gcatatgcag cagatgggga taaaacacga tgagtctcga cgaccagaga
     1741 aatgcctggt tcagaaaaag agagagaaaa agaaaaacac aggccagata aaaattcctg
     1801 gaattggatt ttacggaaaa aatttctccc agaaaaaaaa gaaaggaaag ggcggagctg
     1861 aatgcgatga aggaattgaa aatattgatc tgcagatcag gattcattga atcagattcc
     1921 tatgtcttct tcgtgtgcac tgtttttcat ttcactttct gtcctcgata tcctggagtt
     1981 cgaggctcag agcctttgtg gaaatctgaa tttcccagat ggaaaataaa agagaaaaca
     2041 tcatcagtaa aagactcgtc tgaaaaagaa tttctgcaag tcttttattt tcattgaaaa
     2101 gaaagaacat gcagactaca cagaggagaa atgccaatac accgcaggcc tgcatatttc
     2161 gaatgatcgt aagtctctgc cgcagacttc tgatctgacg cagtatgact tcttccctgc
     2221 tttccagata ctcatcatgc agatcccgga tgagtttagt gagtgcaagg aatctgtttg
     2281 tatatgccag aagaagaagt gaaattgcgg ggaagagaag ggcaggtgtt gaaagtgtca
     2341 gttccataaa gccagactgc aggaaccctg agttttctcg atgataaaat ggttccggat
     2401 tgctgagcct gtgacagaag cccgtttttc tttccgaaat tctgtcaaat accgcttgat
     2461 tctgccgata ttttaaacag agtatccata ctatgaaaat aatcttcggt tttgtcctga
     2521 tttccttttc cctgtacgca gaaaagttta cgatcaagga tgaggcagac taccatgtcc
     2581 atctgaacca taattatgaa tatgaaatgg gcggggtgag gacaaaattt gcagactggg
     2641 ttccctacaa attgcataaa tggaacccta agtttctcga ggatttttat gaactctacg
     2701 gactgcgtct tcattacaat gaaaacgatc tgagaaagga tatctacttt ctgaaaatcg
     2761 gtctgaagag cagattcagg catcccagaa atgctctatg caaaatcacc gacgaagaga
     2821 cctattacaa gtacagactc ctgctgacga tgcatctgaa tcttcagatt atgaggtcgt
     2881 acatgaggat tgcctcccag tacgacaaaa gacatctgta tttttacaat ctcgactttg
     2941 cccatgaact gaaaaattca tttacgaaat ccaaagaaat ttacaaggaa gctctgatct
     3001 actggaat
//
LOCUS       DAOQGJ010000257         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19674_length_3002_cov_3.355955, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000257 DAOQGJ010000000
VERSION     DAOQGJ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19674_length_3002_cov_3.355955"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1578
                     /locus_tag="PK683_05285"
     CDS             214..1578
                     /locus_tag="PK683_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07893.1"
                     /translation="MSCLKNSRSEQKEFFFIDQKKITQEEYSICISKRSCRIKPSGKA
                     FSLSAEMAFQYCRFAGKRLPSRTELEASKDILADYKSDSAEWTSDSEKDSASSGRCAS
                     SSPYLTHAPAWMIQKPMPEKTALPSSINAEQTETFHKLAELDELNKPFCKHLYTSPAH
                     CRDPVSYVYTNESRNYLFGPYIKNLGGGYVGIAADANYTFIAHAKSEWVWLMDFDYVI
                     VHLHKMIRAFVLESDTPAKFASFFNPAKSGAAIELLKRSHNEEEIAVMKKVLQRYGKN
                     LYEHYQKIQLPFSESPDFGWLRNPKAYAYIRTLFQKNRISIQGGDLLKDKSLFSIGNA
                     AKKLGVKIRVFYPSNAEEFWEFSPNYKRNISNLPFDEGSVTIRTVHEYPWQEADRKNG
                     VAGFWHQTVHGAANWQKKLMSPKYTIIDHFKKDRIFPDDTKDFSTIDLPSNVPDSVYG
                     LSGN"
     gene            complement(1699..2073)
                     /locus_tag="PK683_05290"
     CDS             complement(1699..2073)
                     /locus_tag="PK683_05290"
                     /inference="COORDINATES: protein motif:HMM:NF018826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HNH07894.1"
                     /translation="MIEQEHKKVKSGAGFPDYIKAIKRIGVTAFETWVIDGHTVYFGK
                     DNFRTVSPPKYDKLMISHSVNKEKFVDYLKKHQQGKTDYFTFCTHCAETGIEKWIVNL
                     EKMICTYFDQKNSEIFEEGIPQ"
     gene            complement(2344..2622)
                     /locus_tag="PK683_05295"
     CDS             complement(2344..2622)
                     /locus_tag="PK683_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07895.1"
                     /translation="MDWEYILRDAVKGNTIKELHLRRVPSLKTCEDWKKVIEIGLIDH
                     KTKYAHYKGGLVKYGESIYFVAEERLEAIAPYRAWNFKSKIKVTEAEA"
     gene            complement(2787..>3002)
                     /locus_tag="PK683_05300"
     CDS             complement(2787..>3002)
                     /locus_tag="PK683_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017854540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNH07896.1"
                     /translation="ARKEVPVEVSLAYGGAKFQFGSEYIIPKPFDRRVYFEVSTAVAE
                     AAVKCGSARIQYPGREEYRKILEKRIG"
BASE COUNT          963 a          634 c          538 g          867 t
ORIGIN      
        1 cctctgccag gagttgctcc ggctgactcc agaaacagaa gagttgaaat ctacgtttca
       61 gcaaaagagt aattatttta ctgcggaggc atcacctccg cattttccaa tatgaaaaat
      121 ttttccatta tcattccagc aatactgttt actttccaat gttcaagtct atcattcaga
      181 actgaaaatc tctgctcact ttctcctgca ggtatgtcct gtctgaaaaa cagcagatca
      241 gagcagaaag agtttttttt tattgatcag aaaaaaatta ctcaggaaga atattccatc
      301 tgcatttcca aaagaagctg cagaataaaa ccttccggca aggcattcag cttaagtgca
      361 gaaatggcat ttcaatactg ccgttttgca ggcaagagac ttccatcccg aacagaactc
      421 gaagcatcca aagatatatt agccgattat aaatcggatt ctgcagaatg gacatctgat
      481 tcagaaaaag actctgcctc ttccggcaga tgtgcaagca gttctcctta tctcacccat
      541 gctcctgcct ggatgattca gaaaccgatg cctgaaaaaa ctgccctgcc ctcttccata
      601 aatgcagagc agactgaaac gttccataaa ctggctgaac tcgacgaact gaacaaaccc
      661 ttctgcaagc acttatatac atcccctgct cattgcaggg atccagtcag ctatgtctat
      721 acaaatgaaa gcaggaacta cttattcggt ccatatatca aaaatctagg cggcggttat
      781 gtaggaattg cagcagatgc aaactacact ttcattgcac atgcaaaaag cgaatgggtc
      841 tggctgatgg attttgacta tgtcattgta catcttcata aaatgatccg tgcctttgtg
      901 ctggaaagtg atacccctgc caagttcgca tcctttttca atcctgctaa atcaggtgcg
      961 gctattgaac tgctgaaaag atctcataat gaagaagaaa ttgctgtaat gaaaaaagtt
     1021 cttcagagat atggaaaaaa tctttatgaa cattaccaga agatccagct tccattttcg
     1081 gaatcacctg attttggatg gcttagaaat ccgaaagcat atgcatacat cagaacctta
     1141 ttccaaaaaa acaggatctc aatccagggc ggtgatcttc tgaaggataa gtctctgttc
     1201 agtatcggaa atgcagcaaa aaaactcggt gtcaaaatca gagtgtttta tccaagcaat
     1261 gcagaagagt tctgggagtt cagtcctaac tataaaagaa atatttcgaa tcttcctttt
     1321 gatgaaggct cggtcactat ccgaacagtg catgaatatc cttggcagga agcggacagg
     1381 aaaaacggtg tagcagggtt ctggcatcag actgttcatg gcgcggcaaa ctggcagaaa
     1441 aaactgatga gtccaaagta tacaatcatt gatcatttca agaaggacag aatttttcct
     1501 gatgatacaa aagacttcag tacaatagat cttccatcga atgttcctga ttcggtgtac
     1561 ggactctctg gaaactgatc agaaataaat ttacagaata aagcctgagc cgcaatattt
     1621 ttcggttcgg gctttcgtat ttctgagcag catcacacag actcgtcata aatataaaat
     1681 gaagaaaaag ccatatcctt actgaggaat accttcttca aaaatctcag aattcttttg
     1741 gtcgaagtaa gtgcatatca ttttttcaag atttacaatc catttttcaa ttccggtctc
     1801 agcacagtga gtgcaaaagg taaaatagtc ggtctttccc tgctgatgct ttttaagata
     1861 atcgacaaac ttttctttat tcactgaatg ggaaatcatc agtttatcgt atttaggcgg
     1921 agagactgtc cggaaattat cctttccaaa ataaactgta tggccgtcaa taacccaagt
     1981 ttcaaaagct gttacaccaa tccgttttat cgctttaata taatcaggaa aaccggcacc
     2041 cgattttact tttttgtgtt cttgttctat catttcaaca gtaaacatgt ttactatgaa
     2101 aaactcaatg gatgcaggga tcaaataaaa atagcagaat cattacaaaa aagatacatt
     2161 gaaatctttg gcctaaatag aaattttaga agaataagtc ctgaacagga tataattgat
     2221 atcagatttc aggattgttt tctgaaaaga ctcatagaac ttcagaggat tgaaagaacg
     2281 cagtacaaac agatcagtgc tgtcaaaaaa cagcggaatg aaaaaatgca aaacctgatg
     2341 aaatcaggct tctgcctctg taacttttat ttttgattta aaattccatg ctctgtaagg
     2401 agcaatcgcc tcgagtcttt cttcagccac aaaataaata ctttcaccgt atttcacaag
     2461 accgcccttg tagtgggcat atttagtctt gtggtcaatc agaccaatct caattacctt
     2521 cttccagtct tcgcaagtct tcagagaagg cactctgcga agatgaagct ccttaattgt
     2581 atttcctttt actgcgtccc taagaatata ttcccaatcc atttaaaaaa cctctgccag
     2641 actaaaatcg gatatatagt ctgctaatat atgactgtaa aatcaatgag ttttaacact
     2701 gcatctcaaa aattttacta aattccactc gcaactactt tttccctatc tgataaaaag
     2761 tcaagcgaaa cctgcagatg atagaatcat ccaatccttt tttcaagaat ttttctgtat
     2821 tcttccctgc caggatactg gattctggca gacccgcatt ttacagcagc ttctgcaacg
     2881 gcagtggata cttcaaaata aactcttctg tcaaacggtt taggaatgat gtattcactt
     2941 ccaaactgaa attttgctcc gccgtaagcc aagctgactt ctacaggcac ttcttttctg
     3001 gc
//
LOCUS       DAOQGJ010000258         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19713_length_2998_cov_2.869861, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000258 DAOQGJ010000000
VERSION     DAOQGJ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19713_length_2998_cov_2.869861"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1097)
                     /locus_tag="PK683_05305"
     CDS             complement(201..1097)
                     /locus_tag="PK683_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07897.1"
                     /translation="MKQIAFLLLLYLINCFTVYKKDETVLKRETLITKDIKEERDVYI
                     LAPTLNQKGLHLFLDAYRVEEKGKVNFIREKYLENKDFKLEDKYSLGNKECRGNECIG
                     WAIIVTTYMALELSTIPVRLISFPQERIREDIKKGNVSKIKSISTDDVYFILSDKNSD
                     KKYNFQNDRIFIPTVELNLNSNNSKNFPYQLADNKTKKIILSGSFDFTESISKYEELE
                     AVLEENNLQNKLSECQLMFPNFSSSAVTYSKAKLEMENLCSKYAAREVSDPNQSQSKV
                     TYCYNNFDGCYYLTKAAKKTKN"
     gene            complement(1094..1459)
                     /locus_tag="PK683_05310"
     CDS             complement(1094..1459)
                     /locus_tag="PK683_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH07898.1"
                     /translation="MSNAKSSSAALDAVFTALADPTRRAILARLAKGESTVTQIARPF
                     KMSQPAISRHLKVLEQAGLISTAVRAQERPRRLETAPLKKAADWIEKYRQMWEDRYQA
                     LDGLLEELQKLQTQGSQEQ"
     gene            1540..1725
                     /locus_tag="PK683_05315"
     CDS             1540..1725
                     /locus_tag="PK683_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07899.1"
                     /translation="MTKKFESYLSNHRMLSNDLSFFRKAAAVKKILLKDIMPDNLDLT
                     GILFNDSGMQVPYFQNE"
     gene            1781..2686
                     /locus_tag="PK683_05320"
     CDS             1781..2686
                     /locus_tag="PK683_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNH07900.1"
                     /translation="MTDKSRLDFFAVPGQFTESGNYLTILKKLPDSIPELTAVVQGLT
                     VHMFTAESFYGCAVSDARTESEAHIRHFDLLLRKIIELDSSDISKPRKPEKRITGVCH
                     HFSKLLTGILRAKKIPARMRYGFGSYFNEGYFEDHSVCEYWNKKEKRWIIVDPQFDKV
                     WIRNLKIKHNVFDVPNNKFFKPAESWILCREGKLDPLKFGIFEPEMRGLWFIAGNLVK
                     DAAAMHKMELLQWDAWSGMPRPNNSMTNKKTLKFFDELAFLMTEPDKNSVNISEMYEN
                     IKVPDRVFNAMHRHLEWVVDKPKKK"
BASE COUNT          886 a          561 c          605 g          946 t
ORIGIN      
        1 gctgatctcg aatcagacga ataggaaatt tttacccagt aactgaaaac gctcttcacc
       61 tatctgccga tatttctaaa tccagttcgt ggattttaac tattgagacg tttttgaagt
      121 aagtaaagat ttcttattgt ataatccttg taaaatattt tggtatctgt cttttcgagg
      181 tgacagatga ttattctgat tcagttcttc gttttcttag cggctttggt cagataataa
      241 cagccgtcaa aattattata gcagtacgta actttgcttt ggctctgatt aggatcgctg
      301 acctcacggg ctgcatattt agaacagaga ttttccatct ccagttttgc tttactgtat
      361 gtaaccgcgg aactgctgaa attaggaaac atcagctgac attcagaaag tttgttctgg
      421 agattgtttt cttcaagcac tgcttcgagt tcttcgtatt tggaaatact ttcagtaaaa
      481 tcgaaactgc ccgaaagtat aatttttttt gttttattat ctgctagctg atatggaaag
      541 tttttcgaat tattggaatt cagatttaat tcaacagtcg gaataaaaat tctatcgttt
      601 tggaaattat actttttatc tgagttctta tccgagagta taaaataaac atcatccgta
      661 gaaatggatt taattttact gacatttcct ttttttatat cttcccgaat tctttcctgc
      721 ggaaaagata tcaggcgaac tgggatagtg gataattcca aagccatata agtcgttacg
      781 ataatagccc atccgataca ttcatttcca cgacactcct tatttcctaa actatattta
      841 tcttcaagtt taaaatcctt attttctaaa tatttctctc gaatgaaatt taccttgcct
      901 ttttcttcta cacgatacgc atccagaaat aaatgaagtc ctttctgatt cagggtagga
      961 gccaggatat agacatctct ctcttctttt atatcttttg ttataagcgt ttctcttttg
     1021 agaacagttt cgtccttttt ataaacagta aagcagttga ttaaatacag cagaagcagg
     1081 aatgcaatct gtttcactgt tcctgacttc cctgtgtctg cagcttctgc agttcttcca
     1141 gaagtccatc gagtgcctga taacggtctt cccacatctg ccggtatttc tctatccagt
     1201 ctgcagcctt tttgagaggg gcggtttcca gtcttctcgg ccgttcctgt gctcggacag
     1261 ctgtagaaat gaggcctgcc tgttccaata ctttcagatg acgcgagatt gcaggctgac
     1321 tcatcttgaa tggtctggca atctgagtga ctgtgctttc ccctttcgcc aggcgtgcaa
     1381 gaattgccct gcgggtagga tctgctaaag ctgtgaaaac tgcatccaaa gcggctgacg
     1441 aagattttgc attactcatt tgttatataa taatattcga atgtatcaat gtctgtcaag
     1501 aagctttgaa aaaatattat cagagttttt cactgctgta tgacaaagaa atttgagagt
     1561 tatttatcga atcatcggat gctctcaaat gatctttctt tcttcagaaa agctgctgca
     1621 gtcaaaaaaa ttcttttgaa ggatataatg cctgataatc tggatctcac aggaatactg
     1681 ttcaacgatt cgggaatgca ggttccatat tttcagaacg aataaagtct tgacagtata
     1741 gttcatagat aatcaataaa cttggtaaga gtggaaaaca atgacagata aatcaagact
     1801 cgattttttt gccgtccccg gtcagtttac ggagagcgga aactatttga caatactgaa
     1861 aaaactgccc gacagtattc cagaattgac tgccgttgta caggggctga ctgtacatat
     1921 gtttacagct gagtcatttt acggatgcgc tgtaagcgat gccagaacag agtctgaagc
     1981 gcatataagg cattttgacc ttctgctgcg taaaataatt gaactggaca gctcagatat
     2041 ttccaaacca agaaaaccgg aaaaaagaat cactggagtc tgtcatcatt tctcaaaact
     2101 gctgaccggc attttaagag caaaaaaaat tccggccaga atgcggtacg gtttcggcag
     2161 ttattttaac gaagggtatt ttgaagatca ttccgtatgt gaatattgga acaaaaaaga
     2221 aaaacgatgg attattgtcg atcctcagtt tgataaagta tggatcagga atctgaagat
     2281 taaacataat gtatttgatg ttccgaataa taaatttttt aaaccggcag aaagctggat
     2341 tctgtgcaga gaaggaaaac ttgatccttt gaaattcggt atttttgaac ctgaaatgag
     2401 aggactttgg tttattgcag gcaatctggt gaaagatgcc gcagcgatgc ataaaatgga
     2461 acttttgcag tgggatgcct ggagcggtat gccaagacct aataattcca tgacaaataa
     2521 aaagaccttg aaattttttg atgagcttgc attcttaatg actgagccgg ataaaaactc
     2581 agttaatatc agtgaaatgt acgagaacat taaagttccg gacagggtgt ttaatgcaat
     2641 gcacaggcat ttggaatggg ttgtcgataa accaaagaaa aagtagtcat tcactcgctt
     2701 tatgcaggta ggatatgcat tccagcaccg ataacattag gaaaatctag cagtttaaaa
     2761 gcttgtgatg aatcagtaaa aacagagctt ccacgcttgc agcagcttcc acgcttgcgg
     2821 gattttaggc agtttcagat attggcgagg gagaagccca attctgtaca gctagcaata
     2881 ggctttcata tatgttatgc ggaatgatgc ctcgacttgt gctgagcgat gccctgagct
     2941 gtatcgaagg gtcacataga agcatacact tttccccgcc ggtcagctct ctgcaaaa
//
LOCUS       DAOQGJ010000259         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19820_length_2988_cov_2.864644, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000259 DAOQGJ010000000
VERSION     DAOQGJ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19820_length_2988_cov_2.864644"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2002
                     /locus_tag="PK683_05325"
     CDS             <1..2002
                     /locus_tag="PK683_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07901.1"
                     /translation="EQGRAPEAVDTALRTLLDIDARGNQGKERYIYLYPFLLKSANKI
                     TAKIAIIAPQSIHELDVKHYREACFEYSQSMVDLILSNRAKKSKEIDENDPGNSLFQK
                     NKNGQDWLFPNRFSIENEIALLIKSAFKPYGYIPLNDFTEDFIDYGNAAKSLDQITPK
                     YHMILDEIQLKEDGTYVRQPEMVIHYRAQADGLEKFVLKYLPKLAEEAGASEFKTKLG
                     RYTSEHLEKSEPGRKQSREKTKVLIALLKEFPMSKLNSETGKNFKTTCEESIQILEKM
                     IFNMDNLIGRKYNTIQKNLGGSLINYISDYTKNNLKLMVLDVEAEIERSGIKDPEKIK
                     EVRATILKEVKEHYPTRETQDDDGNKIIFVVDQGYMAGVLHKYSSSIKTLPNLSKEFD
                     IAKLMNQEMVQTNNPRLNVNLRKDLVESLNKEAFKNEEQERERNKSEFFKTKFNFLAG
                     GGVFLVCLIISAYFYSAESDIVYMLFGVPISLLFGYLAAVMIKKRPMSGTVTNRDKSF
                     KSVIAESEETNDEKTSQIAKAAAKHIYPSTYNKISDKIYNSESLKEKISETFADIKAS
                     VPILAKEQDISKVASSIEHAVLNSSIIIAIPPEIVPLGMPAQVIVSKADFKSPLLRSQ
                     LSEHYRAEAEKVKLDKALLKYYTFLINTVELEYYKFLNKKIR"
     gene            complement(2015..2500)
                     /locus_tag="PK683_05330"
     CDS             complement(2015..2500)
                     /locus_tag="PK683_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07902.1"
                     /translation="MNWRNIIAVLILSSGSVLSEKQTASVFKGGFFEVEIPSGFSPMP
                     SLGRDKKEGPDSVSFLSSDRKVSFYIFAPQYYYEPEDIIPQPDETLIDEKKENKGGTV
                     HRWFTFKSASGVTRSYHEIRDFDRNRILITGFKFSNHKKYEKYKKRYFLFKKSIRHFD
                     E"
     gene            2643..>2988
                     /locus_tag="PK683_05335"
     CDS             2643..>2988
                     /locus_tag="PK683_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07903.1"
                     /translation="MKILVLIFSLAFTASCAKMKMKSLLDKVSESKTAEEIKSARDES
                     RFLKLEEADVNLAKSGLQKCLNVPADSKNEYTFSAVSKLTSGKNTVLLLKSSHSKAQN
                     MEEVLVSLNENLQ"
BASE COUNT         1015 a          601 c          611 g          761 t
ORIGIN      
        1 cgagcaggga cgtgcaccgg aagcagtgga tacagctctc agaacgcttc tggacattga
       61 tgcaagaggc aatcagggaa aggaaagata catctactta tacccctttc ttctgaaatc
      121 agccaataaa atcactgcaa aaattgcaat catagcaccc cagtccattc acgaactgga
      181 tgtaaaacac tacagggaag cctgtttcga atattcacag agtatggtgg atctgatcct
      241 ttccaacagg gccaaaaaat ccaaggaaat agatgagaat gatccaggaa acagtctttt
      301 tcagaaaaat aaaaacggtc aggactggct ttttccaaac agattttcca tagaaaatga
      361 gatagcactt cttatcaagt cagcattcaa gccatacggt tacattcctc tgaacgattt
      421 taccgaagac ttcatagatt atggcaatgc agcaaaatca ctcgatcaga ttacaccgaa
      481 gtaccatatg atcctggacg agattcagct gaaagaggac ggaacctatg tccgtcagcc
      541 ggaaatggtc attcattaca gagctcaggc agacggtctg gaaaagtttg tactgaagta
      601 tcttcctaaa ctggctgaag aagcaggtgc atccgaattc aagacaaaat tgggacgtta
      661 tacaagcgag catctggaaa agtcagaacc gggaagaaag cagagcaggg aaaaaacgaa
      721 agtcctgatt gcactcttga aagaatttcc tatgtccaaa ctgaattcag aaacaggaaa
      781 gaatttcaag actacctgtg aagaatcgat acagattttg gaaaaaatga ttttcaatat
      841 ggacaacctg atcggaagga aatacaatac gattcagaaa aatctgggcg gttcactgat
      901 aaactatatt tcagactata ccaaaaacaa tctgaagctg atggtactgg acgtagaggc
      961 tgagattgaa agatcaggaa tcaaggaccc ggaaaaaatc aaggaagtac gtgcaacgat
     1021 tctgaaagaa gtcaaggagc actaccctac ccgcgaaact caggacgatg acggcaacaa
     1081 gattatattt gtagtggacc aagggtatat ggcaggcgtt ctgcacaaat acagttcctc
     1141 catcaagact ctcccgaatc tttccaaaga atttgacatt gcaaagctga tgaatcagga
     1201 aatggttcag acaaacaacc ccagactgaa tgtaaacctg agaaaagatc tggtggaaag
     1261 tctgaataaa gaagctttta aaaatgaaga gcaggaaaga gaaagaaaca aatctgagtt
     1321 tttcaagact aaattcaatt tccttgcagg aggcggagta tttcttgtct gcctgatcat
     1381 ttctgcctat ttttattccg cagaaagtga tatagtgtac atgctcttcg gagtcccgat
     1441 cagccttctt ttcggatacc ttgcggctgt gatgattaaa aagagaccga tgtccgggac
     1501 agtcacaaac agagacaaat cattcaaatc agtcattgct gaatctgaag agacaaatga
     1561 tgaaaaaaca agccagattg cgaaagcagc ggcaaagcat atctatccat ccacttataa
     1621 taaaatctct gataaaattt acaactcgga atccctgaag gaaaaaatat cagagacatt
     1681 tgccgacata aaggcatctg tccctattct ggcaaaggaa caggatatca gcaaggttgc
     1741 atcgagcatt gagcatgcag ttctgaattc atcgatcatc attgcaatcc ctccggaaat
     1801 tgttcctttg ggtatgcctg ctcaggtaat cgtaagcaag gcagatttta aatctcctct
     1861 tctgcgttcg cagctctcag agcactacag agctgaagca gaaaaggtaa aactggacaa
     1921 ggcacttctg aaatattaca catttctgat caatactgta gaactggaat attataagtt
     1981 cctcaacaaa aaaatcagat aaccgtcagg cagactattc atcaaaatgc ctgattgact
     2041 tcttaaacag aaagtacctt ttcttatact tttcatattt tttatgattc gaaaatttga
     2101 atccggtaat cagtatcctg ttcctgtcaa agtccctgat ttcatggtag gatctggtga
     2161 caccggacgc tgatttgaat gtaaaccacc tgtgaacagt cccgcccttg ttttcttttt
     2221 tttcatcaat cagtgtttca tccggctgag gaataatatc ttcaggttca taataatact
     2281 gaggtgcaaa tatatagaat gaaacttttc tgtcggaaga cagaaatgaa acgctgtccg
     2341 gtccttcttt tttatcacgg ccaagggaag gcatagggga aaatccggaa ggtatttcca
     2401 cttcaaaaaa gccgccttta aaaactgaag ctgtttgctt ctctgaaaga accgatcctg
     2461 atgaaagaat cagcacagca atgatgtttc tccaattcat aagatcaaca gatctttgtg
     2521 cactcagaat gaacagtatg tttttgaatg gcaaggcaga ctgattcgaa acataaaatt
     2581 ttgaatgaca agggcagcag acgattgaaa ctgggtgcaa acacttgtaa ggaattttat
     2641 ttatgaaaat tcttgtatta attttttcac tggctttcac tgcatcctgc gccaaaatga
     2701 agatgaaatc tcttttggac aaggtaagtg aatcaaaaac tgcagaagaa atcaaatctg
     2761 ccagagatga atcgcgtttt ctcaaactgg aagaggccga tgtaaatctg gctaaaagcg
     2821 ggctgcagaa atgtctcaac gtccctgccg attcaaaaaa tgaatatact ttttctgctg
     2881 tatcaaagct cacttccgga aagaacacgg ttctccttct gaagtcttcc cacagcaaag
     2941 cacagaatat ggaagaagtt ctggtttccc tgaatgaaaa ccttcagc
//
LOCUS       DAOQGJ010000260         2987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19826_length_2987_cov_3.216235, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000260 DAOQGJ010000000
VERSION     DAOQGJ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19826_length_2987_cov_3.216235"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1937)
                     /locus_tag="PK683_05340"
     CDS             complement(240..1937)
                     /locus_tag="PK683_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH07904.1"
                     /translation="MPSEEQDLYNYEQRADFEFNHRENAALHELRVHQIELQVQNEEL
                     KETQHKLEKMRSMYSDLYNNAPVGYLTLNKLGLITRTNRTFLEMIRAEQKNLIGKPFK
                     SLVYLDDQELWISRFKPFWNNPVSKKIEIRLVDDSEIPFYVRVLGRSVYLEDLSKSET
                     TLMIAVIDINSEKIAEQNLNKAKKDAETANSAKSDFLAVMSHEIRTPLNAILGFTSLL
                     IRETYDEQSLESLQIISSSGKLLLSVLNDILDISALESGKLKLVREVFSVRQLIESSG
                     ALAKGMLFSKNKNLDLTLNISPEISEYIVGDYNRILQILNNLISNAVKFTDEGQIDVQ
                     ARLTSDGYIYFSIRDTGSGIPAARQKEVFEPFVQLKEQRKTSFAGTGLGLNIVKRLVE
                     LMGGKVFMTSTIGKLHGSTFTVELPYEPADPPAVKEADEKSLTNFTKCKILIAEDNPV
                     NLNLVSKVLSKSGYETVTAINGIDAVSKFQTETDIGVILMDIQMPVMDGLSAAQEIRR
                     IESENRTFSHTPIVALTATVSSDAQILCHNAGCDRYLTKPLNIDDLLSTIQELLAKN"
     gene            complement(2269..2700)
                     /locus_tag="PK683_05345"
     CDS             complement(2269..2700)
                     /locus_tag="PK683_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07905.1"
                     /translation="MNSKQKILSVLDSLIGEDYEQYPIMPASEKQISIFRKRAESHGL
                     EKKVTDELVSFYEIADGFNYEIVLGFHPCDDELIFEWWDEKTLWLGSRDFYVVRWVDG
                     KYCLGDSSHPSFSKEFEFSSFVEFLDACVWEIRVNMEKNKL"
     gene            complement(2700..>2987)
                     /locus_tag="PK683_05350"
     CDS             complement(2700..>2987)
                     /locus_tag="PK683_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNH07906.1"
                     /translation="EPVIVPLAVPLVAGPSSLATVILLSKQDQNRMHIWFSALLAAWA
                     VSFFILYFSSFIQKIIGDRVLTAIERLMGMILTTMSVQMLLSGLKDFINNL"
BASE COUNT          879 a          633 c          532 g          943 t
ORIGIN      
        1 tcagcaatcc ctgtaatatc attatcagca atattattca tactctgttc cttttgattt
       61 ttgcagaaat cattctgtgc acaggatcca ttattagaat tactggagcc atacttgact
      121 ttataccttt tacggatttt ctgtatttat tataactaat catataattg tttgtactcc
      181 aaatttgtga tccagctgat attttcaata tctatttatc agattcctga ctgcagaatt
      241 caatttttgg caagcagttc ctgaattgtg ctgagcagat catcaatatt gagaggtttc
      301 gtaaggtatc tgtcgcatcc agcattatga cagagaatct gagcatctga cgaaactgtt
      361 gcagtaagag caacaatcgg agtatgactg aatgttctgt tttcggattc aattcttctg
      421 atttcctgag ctgcagaaag accgtccatt acaggcatct gtatgtccat caaaatcacg
      481 ccgatgtccg tttcagtctg aaatttggaa actgcatcaa ttccgttgat tgcagtgact
      541 gtttcataac cgcttttgga aagtaccttg ctgacaagat tcagatttac agggttgtct
      601 tcagcgataa gtattttgca cttggtaaag ttcgtcaatg acttctcatc agcttccttg
      661 accgcaggag gatctgccgg ctcataaggg agttctacag taaaagtgga accgtggagc
      721 ttgccgatcg tcgaggtcat aaatactttt ccgcccatca gttccaccag tctcttgact
      781 atattcagtc ccagacctgt cccggcaaaa ctcgtttttc tctgttcttt cagctggaca
      841 aaaggttcaa atacttcttt ctgtctggca gcaggaattc ctgagcctgt gtccctgatg
      901 ctgaagtaaa tataaccgtc tgacgtcagt ctcgcctgga cgtcaatctg gccttcatct
      961 gtaaatttca cagcgttgct tatcagatta ttcagtattt gcagaatcct gttgtaatcc
     1021 cctacaatat actcacttat ttccggtgaa atattgagtg tgagatccag gtttttattt
     1081 ttgctgaaaa gcattccttt tgcgagagca cctgaacttt cgatgagctg tctcactgaa
     1141 aaaacctctc ttacaagttt aagtttccct gactctaatg ctgaaatatc taaaatatca
     1201 ttaagaactg aaagcagaag ctttccgctg ctgctgatga tctgaagcga ttccagactc
     1261 tgctcatcat acgtctctct gatcaaaaga gatgtaaaac ctaaaattgc attaagaggc
     1321 gttcttattt catgactcat tacggcaaga aaatcactct ttgcagaatt tgcagtctct
     1381 gcatctttct ttgccttgtt caaattctgt tcagcaattt tttcggaatt gatgtcaata
     1441 accgcaatca tcagtgttgt ttcagatttg gaaagatcct caagatagac acttcttccc
     1501 aaaactcgaa cataaaacgg aatttcagaa tcatccacaa gcctgatttc aattttttta
     1561 gagacagggt tgttccagaa aggcttgaac ctggaaatcc aaagctcctg gtcatccaaa
     1621 taaacaagac ttttgaacgg ttttccaatc agatttttct gttctgccct gatcatttcc
     1681 agaaaagttc tgttcgttct ggtaatgagt cccagcttat tcagtgtaag gtatccgact
     1741 ggagcattgt tgtaaagatc ggagtacata ctcctcatct tttcaagctt atgctgggtt
     1801 tccttcagtt cctcattctg tacctgaagc tcaatctgat gaactctcag ttcatgcaga
     1861 gctgcatttt ctctatgatt gaattcaaaa tctgctctct gttcataatt gtacaaatcc
     1921 tgttcttcag agggcattac tgttcaaccg ttcctttgta ccgtgatttt caaaagaata
     1981 tggtccattt tttcaaaaga gaagcttttt taaagtcagt ttgatttttg atcagcacta
     2041 agctatctca gctttatcca cttaagcagt gcaaagtaca gtgtaaagca gtttatcaaa
     2101 taacatacaa actgaacagt ttttacagat ataaaaatac aaattctgct tttttgaaaa
     2161 atttctccgt caaaggtttt aataaaaaag cttaaacaaa atgacggagg cattcaattg
     2221 actcagttta gctgataagt caaaccttgc gaattataaa aaaatatttt ataatttatt
     2281 tttttccatg tttactctga tttcccagac gcaggcatcg agaaattcaa caaagctgga
     2341 aaattcaaac tctttggaaa agctgggatg actggaatct ccaagacagt atttcccgtc
     2401 tacccaccgc actacataaa aatctcttga accgagccag agagttttct catcccacca
     2461 ttcaaaaatc agttcatcat cacagggatg aaaaccgaga acaatttcat aattgaaacc
     2521 gtcagcaatt tcataaaaac tgaccagttc atccgttact tttttttcaa ggccgtgaga
     2581 ttccgctctt ttacggaaaa tgctgatctg cttttccgaa gcaggcataa tcggatactg
     2641 ttcatagtct tcacctataa gtgagtccag aacggataga attttctgtt ttgaattcat
     2701 tataaattat tgataaaatc cttcagtccg gacagaagca tctgcacgga catggtagtg
     2761 agaatcattc ccatcagacg ttcgatggca gtcaaaaccc tgtcaccgat aattttttga
     2821 ataaagctcg aaaaataaag aataaaaaaa ctgactgccc aggcagcaag caaagccgaa
     2881 aaccatatat gcatcctgtt ctgatcctgc ttggaaagaa gaatcaccgt cgccaaggag
     2941 gaaggacctg caacaagagg aacggccaag ggaacaatca caggctc
//
LOCUS       DAOQGJ010000261         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19884_length_2980_cov_2.625641, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000261 DAOQGJ010000000
VERSION     DAOQGJ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19884_length_2980_cov_2.625641"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PK683_05355"
     CDS             <1..552
                     /locus_tag="PK683_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07907.1"
                     /translation="TDPSGKKVKASSIIKVKTSLEGTEKSVLDISSPREFSGNFVRNL
                     LLVFLAVTAVLGAVSYFAFRRRLKAKTAKDADFDFIQKEEISFKDKIEELLKQQEVQH
                     KEFIYLLTGFIKESLGKKLDKNLVYLDETELGRIMREMKLLKEFEIMQMETYFQSVKY
                     MPNEDLIPVRKADEIYYYWKHKI"
     gene            518..1492
                     /locus_tag="PK683_05360"
     CDS             518..1492
                     /locus_tag="PK683_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH07908.1"
                     /translation="MKFIITGSIKSDMSFEREYILYFIPAVMIYAWLFFRFRFHRNRE
                     QITASAQNLNSGKTWRDRLTDMIPLFRFIGFVFLLIALAGPGQKAELLPDEQNGVDIM
                     LALDVSGSMTVTPDFAPSNRLEVSKNLMKEFVKKRITDRMGLVVFAGAAYLQSPLTSD
                     QSSLQEIISDVHPGSVEEQGTAVGDAVILCTYRLKQSKARSRIIVLLTDGASNAGKVD
                     IITSSEASKEFGIRIYSIGIGTDRSAETDFESLEKISEKTNGKFYRAHDPAELETVFS
                     DIDSLEKDILNSSPKTILRTQFELFLYIALICFCLDLMLRTFIGRSYV"
     gene            1485..2540
                     /locus_tag="PK683_05365"
     CDS             1485..2540
                     /locus_tag="PK683_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH07909.1"
                     /translation="MFNSFKNLNSFSVSFTVLVFIVFLIWVIQRVRSRMIIKEISEIH
                     PSLKKSFSTAPNYIHYIRFFIIFLILGFCSFALLNPSFSKPAGESERKSEGVDILFLI
                     DVSLSMYAVDAPPSRLQKVKESILHLLPSLEGNRLGIITFAGTAFLYCPMTKDLGAFS
                     DFLRGLEADMIPDTGTDMQKAFAKAEEVIESKKILRNRILVLITDGENKNQSFPKIKN
                     ADMIVIGVGTSQGDAIYYRDESAKLAGYVTKSRHLVQRKEGDLVISKADEEYLAELAS
                     KNSAVYRNLSREPDSMQIIKDRISEMEKNQSEDIRKYARLDGYQYFLYPAVLFLLIDL
                     LFMEILLKKILRKKGTV"
     gene            complement(2553..>2980)
                     /locus_tag="PK683_05370"
     CDS             complement(2553..>2980)
                     /locus_tag="PK683_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07910.1"
                     /translation="SRSALRDSIDSILSQFGNHFAVKKFLAKELIHQNEYGLALELSE
                     SGVLENPNDNTLLFLSAYSAFKTGIYRKTLSYAYRLHYRTDSSVDNTLLICRTYHKMS
                     DYHSLFDFTSKVVYTDKYTNEDVMKYHKYAHARLQEENV"
BASE COUNT          913 a          585 c          639 g          843 t
ORIGIN      
        1 acagatcctt ccggcaaaaa ggtcaaggct tcttcaatca tcaaagttaa aaccagcctt
       61 gaaggaacgg aaaaaagcgt tctggatatt tcttctccca gggaattttc agggaacttt
      121 gtcagaaatc tgcttctcgt atttcttgca gtcactgctg ttctgggagc cgtttcctac
      181 tttgccttcc gcagaagact gaaggcaaag accgcaaaag atgcagattt tgactttatt
      241 cagaaggaag aaatctcctt caaggacaaa atagaggagc ttctcaagca gcaggaagtt
      301 cagcataagg aattcattta tcttcttaca ggattcatca aagaatccct tgggaaaaaa
      361 cttgataaaa atcttgttta tctggatgaa accgaactcg gaagaattat gagagagatg
      421 aagctgctga aggaatttga aattatgcag atggaaacat actttcagtc cgtcaagtat
      481 atgccgaatg aggatctgat cccggtccgg aaagcagatg aaatttatta ttactggaag
      541 cataaaatct gatatgagtt ttgaaagaga gtatatactg tattttattc cggctgtcat
      601 gatttatgca tggctttttt tcagattcag atttcacaga aacagagaac agattacagc
      661 atcagcacag aacctgaatt caggcaaaac ctggagggac agacttacgg atatgattcc
      721 tctgttccgt ttcatcggtt ttgtttttct tttaattgca cttgccggcc ccggacagaa
      781 agcagagctt cttccggatg aacagaacgg agtagacatt atgctggcac tggatgtctc
      841 cggctcaatg actgtgacac ctgattttgc tccgagcaac agactggagg tttccaaaaa
      901 tctgatgaag gaatttgtga agaaaagaat cactgaccga atggggcttg tagtgtttgc
      961 aggagctgca tatcttcagt cccctcttac atcagatcag tcctctcttc aggaaatcat
     1021 atctgatgtt catccaggct cggtcgagga acagggaaca gcagtaggag atgcagtgat
     1081 actctgcact tacagactga aacagtccaa ggcaagatcc agaatcattg tgctgctgac
     1141 tgacggtgca tcgaacgcgg gaaaggtgga tatcattact tcctctgagg cttcaaaaga
     1201 gtttggaatc aggatttatt cgattggaat cggaacagac agatccgctg aaaccgactt
     1261 tgaaagtctc gaaaaaatct ctgaaaagac gaatgggaaa ttctacagag cacatgatcc
     1321 cgctgaactt gagacagttt tttctgatat tgactctctc gaaaaagata tcctgaacag
     1381 cagtccaaag acaatcctga ggacccagtt tgaacttttc ctgtatattg cactcatctg
     1441 tttctgtctg gatctgatgc ttcggacatt tataggcagg tcctatgttt aattcattca
     1501 aaaatctgaa cagtttttct gtaagcttca ctgtattagt ctttatcgta ttcctgatct
     1561 gggtcatcca gagagtcaga agcagaatga ttataaagga aatttcagaa attcatccaa
     1621 gcctgaaaaa gtccttctca actgctccaa attacataca ttatatacgc tttttcatta
     1681 tttttttaat attgggattc tgttcttttg ctctgctgaa tccgtccttt tccaagccgg
     1741 caggagaatc agaaagaaaa tcagaaggag tcgatattct ctttctcatt gacgtcagtc
     1801 tttccatgta tgcagtagac gctccgccga gcagacttca gaaagtaaaa gaatcaattc
     1861 ttcacctgct cccatccctg gaagggaaca ggctcggaat tattacattc gcaggaactg
     1921 catttcttta ctgcccaatg accaaggatc tgggggcttt ttcagatttt ctcagaggac
     1981 ttgaagcaga tatgataccg gacaccggca ctgatatgca gaaagctttt gcaaaagctg
     2041 aagaggtcat tgaatcaaaa aaaatactga gaaacaggat tcttgttctg atcactgacg
     2101 gtgaaaacaa aaatcagtct tttcctaaaa taaaaaatgc agatatgatt gtaatcggcg
     2161 taggcacatc acagggcgat gcaatttact acagagacga gagtgccaaa cttgcaggat
     2221 atgttacaaa atccagacac ttggtgcaaa gaaaggaagg agatctggtc atcagcaagg
     2281 cagatgaaga gtatcttgct gaactggcct ctaagaacag tgctgtttac agaaacctca
     2341 gcagggaacc tgattcaatg cagatcatca aggacagaat ttcggagatg gagaaaaatc
     2401 agagtgagga tatcagaaaa tatgcgagac ttgacgggta tcagtatttc ctgtatcctg
     2461 cagttctgtt tctcctgatc gatctgcttt ttatggaaat tcttctgaaa aagattctga
     2521 ggaaaaaagg aactgtgtaa catattcagc atttacacat tctcttcctg aagtcttgcg
     2581 tgagcatatt tatggtattt catcacatct tcatttgtat atttgtcggt atagactacc
     2641 tttgaagtaa aatcgaaaag agagtgataa tcactcattt tatgataagt tctgcagata
     2701 aggagagtat tgtccacact tgaatcggtc ctgtagtgaa gacggtatgc ataagagaga
     2761 gttttcctgt atatgcctgt cttgaaagca gaataagctg aaagaaaaag aagagtattg
     2821 tcattcggat tttccagcac tccgctttca gaaagctcca aagccagacc gtattcattt
     2881 tgatgaatca gttcctttgc gaggaacttc tttacggcaa aatgatttcc aaactgagag
     2941 agtatgctgt ctatactgtc tctcagtgca ctgcggctcc
//
LOCUS       DAOQGJ010000262         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19896_length_2979_cov_3.266758, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000262 DAOQGJ010000000
VERSION     DAOQGJ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19896_length_2979_cov_3.266758"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1386)
                     /locus_tag="PK683_05375"
     CDS             complement(169..1386)
                     /locus_tag="PK683_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNH07911.1"
                     /translation="MNLQYLTLFSILIFFINFLNILFAFTKSTANVARWSVIVILFTA
                     VQIVSWYAGDFALQWVLIEACTLFGALLISMSENEKSTAVAWKFLLLNSFGMGIAFLG
                     LIILSFGIHSVVTMDIETIMKNIASEQNSFVEIGIWLAVFGYSVKLGLVPNQYWVSDT
                     YSESPSQISSLFASMIPVSVSIALRPIIQMDILMQNPHLNARHGLLVMGIATMIYSIL
                     TLYQAKDIRRITSMIALFHNGAIGVFLWLNTSDEVFYLALTSLVVIKTLIFSSMGILR
                     LDISSRFLSEISPSEGVSSVSRILFLSSVFMAFALPVSPMFLSDMMALKNAVKHSYYW
                     YTLVPGLGICFFAITLYKILPVMNIESRGFRPETRTKITVRAILAGAVFLLAFLLSMF
                     GFYMFAYKGLGNV"
     gene            complement(1383..2000)
                     /locus_tag="PK683_05380"
     CDS             complement(1383..2000)
                     /locus_tag="PK683_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenase"
                     /protein_id="HNH07912.1"
                     /translation="MIFDIIYLLLLITGIIILVENRLGRVIALCSVQGLLLCGPVFQV
                     YSPYEFHSWALTALILVFKTFLTPYILFYYLKQVRLFEHVKPRFGYQVSIFLFLIGLF
                     AALSISRSIHNMPVHIDRIGLIYVILLIYLGILTFIASRHWIVLIIGFVMFENGVFLL
                     TLILHKGLPFGTEIVSFADAFLVIVAAAVLQRKVDSFKNSSGSRE"
     gene            complement(2026..2907)
                     /locus_tag="PK683_05385"
     CDS             complement(2026..2907)
                     /locus_tag="PK683_05385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNH07913.1"
                     /translation="MTEKIINIVLQILGFIIIPVFAEAVIRKVRARAQGRKGPPLLQN
                     LYDFQRLFQKKPVDGPYSGVFCENAPAFTLLASILVWASVLFGWPVMLSIPFFLALQR
                     IAMTGYAMETGTSFGGLGSSREILLFVSAEPVIIMIILVGQSRLNLDFSPESVIVGTL
                     FLSAGLVAVLAELARPPFDDPRTHLELTMVHEAMLLEASGRRLALFDAAYQIKISALL
                     YLLIKLALDHSKLPFAESLPVYAYTLAAFGGTVFLSALTGYWESASIRRRWDWVPEIM
                     GLTFLFLLILGTLVKIK"
BASE COUNT          919 a          661 c          672 g          727 t
ORIGIN      
        1 ctgtgtctga gaacaggaag aggaatttcc ggatctctga gagagtcttc cagactgaat
       61 ttttccgctt ccttcattga aaccgatgct gcattctgct caaaaagaac agtcctgccg
      121 tcggaattct tcctgaatgc cccagtgtat tttctgaatt tcagcgtttc agacatttcc
      181 gagccccttg taagcgaaca tataaaaacc gaacatagaa agaagaaagg ccagaaggaa
      241 gactgctcct gccaggatgg ctctgacagt tattttggtt ctggtctccg gtctgaatcc
      301 tctcgattca atattcatta ccggaagaat cttgtacagt gtgattgcaa agaagcagat
      361 tcctagacct ggaacgagag tgtaccagta ataggaatgt ttcaccgcat ttttcagtgc
      421 catcatatcc gaaagaaaca taggcgatac aggaagggca aatgccatga acaccgaaga
      481 gaggaaaaga attctggata cggaactgac tccctcgctc ggactgatct cgctcaggaa
      541 tctgcttgaa atatccagac ggagaattcc catactgctg aatatcagag tcttgatgac
      601 tacaagactg gtaagggcaa gatagaatac ttcatctgaa gtgttcagcc agagaaagac
      661 tcctatcgca ccgttgtgaa aaagtgcaat catggatgta attcttcgaa tgtcctttgc
      721 ctggtacaga gtaagtatgc tgtatatcat tgtcgctatt cccatgacaa gaagtccgtg
      781 tctcgcattc agatgcgggt tctgcatcag gatgtccatc tgaatgatcg gacggagcgc
      841 aatggatacg gatacaggaa tcatggatgc aaacagggaa gagatctgac tcgggctttc
      901 cgaatatgta tcgcttaccc agtactgatt cggaaccagt cccagtttga ctgagtatcc
      961 gaaaactgcg agccagattc cgatttccac gaatgagttc tgctctgacg ctatattttt
     1021 cattatggtt tctatatcca tggtaacgac agaatggatt ccgaaggaaa ggatgatcag
     1081 acccagaaat gcaatgccca ttccgaatga attcagaaga aggaatttcc aggcaactgc
     1141 agttgatttt tcattttcgc tcattgagat caggagagct ccgaaaagag tgcaggcctc
     1201 tatgagaacc cactgcagtg caaaatctcc tgcataccag gacacaatct gaacggcagt
     1261 aaaaaggatt acgatcaccg accatcttgc tacatttgca gtgctcttgg taaatgcaaa
     1321 aaggatgttc agaaaattga tgaaaaatat cagaatactg aataaagtaa gatactggag
     1381 gttcattctc tgctccctga actgttctta aatgaatcca cttttctctg cagaactgcg
     1441 gctgccacaa tgaccaggaa agcatctgca aatgatacga tttctgtgcc gaaaggcaga
     1501 cccttgtgaa ggatcagagt cagaagaaat actccgttct caaacatcac gaatccgatg
     1561 atcagcacaa tccagtgcct gctcgcaatg aacgtcagga tgcccagata gatgagcaga
     1621 atcacgtaaa tcagccctat cctgtctatg tgcaccggca tattgtgaat ggatctgctg
     1681 atgctcagtg cagcaaacag tccgatcagg aataaaaata tggagacctg atatccgaac
     1741 ctgggtttca catgctcgaa aaggcgtacc tgcttcagat aataaaaaag aatatagggg
     1801 gtgaggaaag tcttgaaaac cagtatcagt gcagtcagag cccaggaatg aaactcatac
     1861 ggactgtata cctgaaagac cggaccgcag agaagaagtc cctgaacgga acagagagca
     1921 atgactcttc cgagtctgtt ttccaccaga ataatgatcc ctgtaatgag caggagaaga
     1981 tatataatat caaaaatcat aattatttcc aatcctctga atgtttcatt ttatcttcac
     2041 cagagttccc agaatcagaa gaaacagaaa cgtaagtccc ataatttcag gcacccagtc
     2101 ccagcgtctc cggatgcttg ctgattccca ataaccggtc agtgcactta aaaagactgt
     2161 tcctccgaac gcagcaaggg tataagcata taccggaagt gattctgcaa aaggcagctt
     2221 ggaatgatcg agggccagtt ttatcagaag gtacagaagc gcggaaattt tgatctgata
     2281 ggctgcatca aacagtgcaa gacgtctgcc ggaagcttcg agtagcatcg cctcatgaac
     2341 cattgtcagc tccagatgag ttctgggatc gtcaaacggg ggtcttgcga gttctgccag
     2401 aactgccaca agtcctgcgg aaagaaaaag tgttcccacg atcactgatt ccggcgagaa
     2461 atccagattc agtctggact gtccgacaag aatgatcatt atgattacag gttctgctga
     2521 tacaaacaga agaatttctc tggatgatcc gagacctccg aaagaagtcc ccgtctccat
     2581 tgcataacct gtcatggcta ttctctgcag tgccagaaaa aatggaatgg aaagcatcac
     2641 aggccagccg aaaagaaccg atgcccatac cagaatggat gccagaagag taaatgctgg
     2701 agcattttcg cagaatactc ctgaataagg accgtccaca ggcttttttt gaaaaagtct
     2761 ctgaaaatca tagagattct ggaggagcgg aggacctttt cttccctgtg cccttgctct
     2821 gactttccgt attacagcct ccgcaaatac gggaataata ataaatccta atatctgtaa
     2881 aactatatta atgatttttt ctgtcatagt ctgaatcctg aaacagtctg atatacgaga
     2941 agaacggcga cggaaacaag aagtgccgca gcggagaag
//
LOCUS       DAOQGJ010000263         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19899_length_2979_cov_2.459302, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000263 DAOQGJ010000000
VERSION     DAOQGJ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19899_length_2979_cov_2.459302"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1768)
                     /locus_tag="PK683_05390"
     CDS             complement(<1..1768)
                     /locus_tag="PK683_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH07914.1"
                     /translation="MNRVCFTDLIHSEDINLFRIGCRAILTGEAPKFKIELRINGRKN
                     SLVWSDWEFSVRHYGGDLPAIVGLGKDITEFKKNEIVILSSEREKQKVIEEELKRNRE
                     RYSAVIDSMQEGVIIYDLEGKIIDCNRSAERILEFRRSELLGLTHSEPVWKIIKEDKT
                     DFMPEEIPVVSSLRTGLSYRNTIMGIYKRSGNLIWISGSSQILCHPESGIPFGAAQIF
                     HDITEMKIADEKLRANREFLNNLIDSLPGYVYRVANDSAYTPLYISESVYEITGYTQS
                     EYLTEKTILCGREIHPEFAERVRESLQKALDEKKPYEFEYKIITKSGSEKWVWEKGRG
                     IFDDSGQPRYLEGFVSDITDTISLKEKLISERGRLYDIIEAANVGTWQWNIRTGEVII
                     NKKWTEIAGYGFTELMPFTMKVWISLIHPDDFKISRTLMKKHLAGKTDFYLCEIRIKH
                     KDGNWVWVLSRGKVLSRKNGKLFLMSGTIQDINSKKVAEEKNKEILDRFTKIALHLPG
                     FIFLFRMRPDGTFDLPYASNGIKEIFEIDPEDVKDDGAPIFRIIHPDDKELVISSILA
                     SAASLTPWQQEWRIIQKDGRVLW"
     gene            complement(2045..>2979)
                     /locus_tag="PK683_05395"
     CDS             complement(2045..>2979)
                     /locus_tag="PK683_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNH07915.1"
                     /translation="SIFSVNPGTWKIKFASAGYVPVEGIITVQSGATTNFDIVTMIPG
                     SQAPASISGRLLNAQNNTAITTGLTVRIRPGVGVTSGPYALDSSNATIGAVTSAADGS
                     YVIPNVPAGNYTVEVSGTGYATTYQTVISAGANSANQNILVSPVLGATEVRIVLSWNA
                     TPRDVDSHLEFDAGPYNQVVWNQKNRLCSNATVESAKGSSQPLRTDICDLTLDYDVVT
                     GYGPETVTMKNTVWTKPRRGYSLYNWSNEASLSTSGSTVKVYKSTGLVRTYNVGPAQV
                     GRWWQMFCLQADRTIIDVGQTGCSASSFFNTPQN"
BASE COUNT          836 a          676 c          535 g          932 t
ORIGIN      
        1 gccataaaac tcttccatct ttctgaataa ttctccactc ctgctgccag ggagtcagag
       61 aagcggccga agcaaggatt gacgatatga ccaattcttt gtcatcggga tgaataatcc
      121 tgaaaattgg ggctccatcg tctttcacgt cttcagggtc aatttcaaag atttccttaa
      181 tcccgttgct cgcgtatgga agatcaaatg tcccgtcagg tctcatacgg aaaagaaaaa
      241 taaatccagg aagatgcaga gctatttttg taaatctgtc caatatttcc ttattttttt
      301 cttctgcgac tttttttgaa ttgatatcct gaatcgtccc tgacataaga aagagttttc
      361 cgttctttct tgaaagaacc ttccctctgc tcagaaccca gacccagttt ccgtctttat
      421 gcttaattct tatttcgcag agataaaaat ctgtcttccc cgcaagatgt ttcttcatca
      481 aagttcttga gattttgaaa tcgtccggat gaatcagtga gatccacact ttcatagtaa
      541 acggcatcag ttcagtaaaa ccgtatcccg caatttcggt ccatttcttg tttataatga
      601 cctcaccggt ccggatattc cactgccatg tccctacgtt tgccgcctcg atgatatcat
      661 aaagtcttcc tctttccgaa ataagcttct ccttgagaga gatcgtatcg gtaatgtcac
      721 ttacaaaacc ttccagatat cttggctgtc cggaatcatc aaatattcct ctgccttttt
      781 cccataccca tttttcagat cctgattttg taatgatttt atattcaaat tcataaggtt
      841 ttttttcatc taaagccttc tgcagagact cacggacacg ctctgcaaat tcaggatgaa
      901 tttctcttcc gcataaaatt gttttttcgg tcagatattc actttgagtg tatcctgtaa
      961 tttcataaac agattcactg atgtacaaag gagtataggc agagtcattc gcaactcggt
     1021 aaacatatcc cggaagactg tcgatcagat tgttcaaaaa ctcccgattc gctctcagct
     1081 tttcatccgc gattttcatt tcagtaatat catgaaaaat ctgcgctgct ccgaagggaa
     1141 ttcctgattc aggatgacat agaatctgag aactgcctga aatccagata agatttccgc
     1201 ttcttttgta aattcccata atggtattcc tgtaagaaag ccctgttctg agcgaagata
     1261 caactggaat ttcctcaggc ataaaatcag tcttgtcttc ctttattatc ttccatacag
     1321 gctcagaatg agtcagtccc aaaagttctg aacgcctgaa ctccagaatt cgttctgcac
     1381 ttctgttgca gtcaatgatc tttccttcca gatcatatat aatcacgcct tcctgcatgg
     1441 aatcaatgac agccgaatac cgttctctgt ttcgcttaag ttcttcttca atgactttct
     1501 gtttttctct ttcagaggaa agtattacaa tttcattttt cttaaattca gtgatgtctt
     1561 ttcctaatcc tacaatggca ggaagatcac cgccataatg tctgactgaa aattcccaat
     1621 cactccagac caggctgttt tttcgtccgt tgattctgag ttctatttta aattttggcg
     1681 cttctccggt caaaattgcc ctgcatccga ttctgaaaag attgatatct tcggaatgaa
     1741 tcaggtcggt aaaacagact ctgttcattt ccttcaaggc tgattcagaa aaataccgat
     1801 tacattgtac tattctgaat tctgaattca attcacagaa aaatataaat tctgaatcga
     1861 taacaaatga aggtacaatt tcagaaaact gctcttcatt cataaatgag ctcataataa
     1921 aatgatttca taataaaatg aatcagatta tccgtgcctt atgtaaaggc ttttttcact
     1981 tcgcagtgta cataaaaaaa agggattcat ttctgaatcc cttttttaag tgatagaact
     2041 gaactcagtt ctgaggtgta ttgaagaaac ttgaagcaga acagccggtc tgaccgacgt
     2101 cgataattgt cctgtccgcc tgaagacaga acatctgcca ccatctgcct acctgtgccg
     2161 gtccgacatt gtaagttctc acaagtcctg tagatttgta aacttttaca gtagaacctg
     2221 atgtggaaag agaagcttca ttcgaccagt tgtaaagact gtatcccctt ctaggcttag
     2281 tccagactgt gttcttcatt gtcactgttt cagggccata tcctgtcact acatcataat
     2341 ccaaagtcag atcgcaaata tcagttctca aaggctgact gcttcctttt gcagactcaa
     2401 cagttgcatt tgaacaaagt ctgttcttct gattccatac aacctgattg taaggacctg
     2461 catcaaattc taaatgggaa tccacgtctc taggagttgc attccaggaa agaacgattc
     2521 tgacttctgt tgcaccgaga acaggtgata cgagtatatt ctgattagct gaatttgctc
     2581 cagccgaaat gacagtctgg taagtagttg catatccagt tccgctcacc tcaacagtgt
     2641 aatttccagc agggacattc ggaatgacat atgacccgtc agcagctgaa gtcacggcac
     2701 caatggttgc atttgaagaa tccagagcgt aaggaccgct tgtcacaccg actccaggac
     2761 gaattcttac agtaagacct gtcgtaatag ctgtattatt ctgagcattc aggagacgtc
     2821 cggaaatcga cgccggagcc tgtgaaccag gaatcattgt cacaatgtca aagtttgtag
     2881 tagcacctga ctgaacagta atgatacctt ccacaggcac atatcccgct gatgcaaatt
     2941 taatcttcca tgttcctgga ttgactgaga atatactgt
//
LOCUS       DAOQGJ010000264         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19911_length_2978_cov_3.244270, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000264 DAOQGJ010000000
VERSION     DAOQGJ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19911_length_2978_cov_3.244270"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PK683_05400"
     CDS             complement(<1..472)
                     /locus_tag="PK683_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07916.1"
                     /translation="MNKKAVLTLILFLSACVTGERTTYVKAEDAEASPEWGPSEIHST
                     VEEMASSMNAFFKKNSNPPYVEIHKLENRTSEHIDLKMLSNEISSSLLKKEIRFVDKS
                     FRKEALNEIAMGQRGIIDEKSKIEAGRLVSPNFKLTGDISENVRFADNKKLQYLS"
     gene            698..1651
                     /locus_tag="PK683_05405"
     CDS             698..1651
                     /locus_tag="PK683_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07917.1"
                     /translation="MKKLKIYLPVLFLLLVSGAVLYALNLFADMLIHPYWHVPGLTAS
                     CSDEQKESSPELCDGRALETVKKKYGDSVEEISLPMEGSGKIQGWHFPLKNADSAVIF
                     VHGAGGDRRNGYRYLPFFSSAGYSYISYDSPNHGISSNDGRGVSYGEREKKAFAQVVR
                     FAESKYKNIFVFASSAGCSSVLLSQNEWKGKVNAVIFENPYYSLRRLVEDNPTAKALP
                     GFFLNLALWNAGRKGGFSVDDIRNADLLEGFPDIPVLVAHGTADAVIPFGHGMDFYGK
                     LKVQNKKFLKGEGAGHSRVWNRIGKEFEKEVLETFKKGEKK"
     gene            1651..2508
                     /locus_tag="PK683_05410"
     CDS             1651..2508
                     /locus_tag="PK683_05410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH07918.1"
                     /translation="MSELGPIVYFVPVFVILTLADFIIGYRKDGFNPKDSAASITMGL
                     GNLGLGFFAKGIAFGVYAWVYQYRFFTIEMNWLGWVLLVLADDFMYYWFHRISHECRL
                     FWASHVIHHSSQAYNLSTALRQTWTGSFYSFIFWMPLALIGFKPEYILLQQAFSLFYQ
                     YFIHTERVNRLGFLEWFMNTPSHHRVHHGSDPKYLDRNHAGIFIIWDRMFGTFQQEEE
                     RPKYGIVKNLESFNPLVIAFHEWISIGRDILKGFSPKSFVGYVFGPPGWQPDGKGTTS
                     DVLRKQAGL"
     gene            2505..>2978
                     /locus_tag="PK683_05415"
     CDS             2505..>2978
                     /locus_tag="PK683_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07919.1"
                     /translation="MIFFLAVPAVGILCIAASVGIMTDVSRDRWFPMLLYLFVPAVSS
                     ILYTLARFGLVFKDTEIPDPSGKMTAAQKSFAVNLTVFFISSVLILIADVPGMADEAL
                     LYTKSKNLILERKEISIISEKGEKFSVQAMIADTPEKQGIGMMAVSELAPNEGMLY"
BASE COUNT          782 a          583 c          705 g          908 t
ORIGIN      
        1 tggaaagata ctgaagtttt ttattgtctg caaagcggac attttcgctg atgtctccag
       61 tgagcttgaa gttgggagac acaagcctgc ctgcttcaat tttcgatttc tcgtcaataa
      121 ttcctctctg tcccattgca atttcattca gtgcctcctt tctgaaggac ttgtccacaa
      181 agcggatttc ttttttcagg aggcttgagg aaatttcgtt ggagagcatt ttcagatcta
      241 tatgctctga cgttctgttt tccagtttgt gaatttcaac gtacggcgga tttgagtttt
      301 ttttgaaaaa tgcattcatt gaagaggcca tttcttccac tgtggaatga atttcggaag
      361 gtccccattc aggacttgct tctgcatcct cagccttgac ataagttgtt ctttcccctg
      421 tcacacatgc ggacagaaac aggatcagtg taagcaccgc ttttttattc attttatcct
      481 cctgtaattt gactgaatca gaaacggttc tttttgaaaa accatctttt tttgtcgact
      541 ggccttccgt cttgactgtc tgtgcagcaa aaaaagtaag ctcataattt ctcagatcag
      601 gaaaagcggc tgtatacagt atggctgttt ttttcgaatg aaatctttct gtcatacaat
      661 aagttgactt ctttgggaag gataggagaa tcggagtatg aaaaaactga agatctatct
      721 tcctgtcctc tttttgcttc ttgtctcagg tgcagtgctt tatgcgctga acctgtttgc
      781 agatatgctg attcatccct actggcatgt accgggactc actgcatcct gttcggatga
      841 gcagaaagag tcttctcctg agctctgtga cgggagggca cttgaaactg tgaaaaaaaa
      901 atacggagac tctgtggagg agatctcact cccaatggaa ggaagtggaa aaattcaagg
      961 atggcatttc cctctgaaaa atgcagattc agcggtcatt tttgtgcatg gtgccggagg
     1021 ggacagaaga aacgggtaca gatacctgcc ttttttctcg agtgccggat attcctatat
     1081 cagttacgat tcaccgaatc acggcatatc ttcgaatgac ggaagagggg tcagttacgg
     1141 agaaagagaa aaaaaagcct ttgctcaggt tgtgcggttt gctgaatcaa agtataagaa
     1201 tatcttcgta tttgcctcct ctgcaggatg ctcttctgtc cttctttctc agaatgaatg
     1261 gaaagggaaa gtaaatgcag tgatattcga gaatccctat tattcactca gaaggcttgt
     1321 ggaagacaat cctactgcaa aggcgcttcc cggttttttt ctgaatctgg cactctggaa
     1381 tgccggaaga aagggcggtt tttctgtgga tgatatccgc aatgcagatc ttctggaagg
     1441 ctttcctgat atccccgtac ttgtggcaca cggcacagct gatgcagtta ttccatttgg
     1501 acacggtatg gatttttacg gaaagctgaa ggtgcagaac aaaaaatttc tgaaagggga
     1561 aggggcaggg cattcccgtg tatggaaccg aattggaaaa gaatttgaaa aggaagtact
     1621 ggagactttt aaaaagggag aaaaaaaata atgagcgagc ttggaccaat tgtatatttt
     1681 gttcctgtat ttgtaatact gaccctggca gattttatca tagggtacag gaaggatggt
     1741 ttcaatccga aagattctgc agcaagcata acgatgggac tgggaaatct gggactcggg
     1801 ttctttgcaa aaggaatagc tttcggcgtc tatgcctggg tgtaccagta cagatttttt
     1861 accattgaga tgaactggct ggggtgggtt ttacttgtgc tggcggatga ttttatgtac
     1921 tattggtttc acagaatcag ccatgaatgc agactgttct gggcaagtca tgtgattcat
     1981 cacagttcac aggcatataa tctttccact gcactcagac agacctggac aggaagtttt
     2041 tactccttca tcttctggat gcctctggca ttgatcggat tcaagccgga atatattctg
     2101 cttcagcagg cattcagtct gttctatcag tattttattc atacggaaag agtgaacaga
     2161 cttggatttc tggaatggtt tatgaatact ccttctcatc acagggttca tcatggaagt
     2221 gaccctaagt acctggatcg caatcatgcg gggattttca tcatttggga cagaatgttc
     2281 ggaacctttc agcaggagga agaaagacct aagtacggta ttgtaaagaa tctggaaagc
     2341 ttcaatcctc tcgtcattgc atttcatgaa tggatttcca tcggacggga tattctgaaa
     2401 ggtttttcac cgaaaagttt tgtcggttat gttttcggtc cgccgggatg gcagcctgac
     2461 ggaaaaggca cgacttcaga tgtgctcaga aaacaggcgg ggctttgatt ttctttcttg
     2521 ctgttcctgc agtaggaatt ctctgcattg ccgcatccgt cggaattatg acggatgtga
     2581 gcagagacag atggtttcca atgcttctgt atctttttgt gcctgcagta tcttctatcc
     2641 tttacactct ggcaagattt ggactggttt tcaaggacac agaaattcct gatccctcag
     2701 gaaaaatgac tgctgcccag aagtcatttg ctgtcaatct gactgttttt tttatcagct
     2761 cagttctcat actgattgca gatgtgcccg gaatggccga tgaagctctt ctgtatacaa
     2821 aatcaaaaaa cctgatactt gaaaggaaag agatttccat catcagtgag aaaggggaaa
     2881 aattttctgt tcaggcaatg attgctgata ctcctgaaaa gcagggaatc ggtatgatgg
     2941 ctgtttctga attggctccg aatgagggaa tgctttac
//
LOCUS       DAOQGJ010000265         2972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_19989_length_2972_cov_2.687007, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000265 DAOQGJ010000000
VERSION     DAOQGJ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19989_length_2972_cov_2.687007"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK683_05420"
     CDS             <1..202
                     /locus_tag="PK683_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004499970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07920.1"
                     /translation="GEYSFVGAGTLITPGKKIPPGVLVMGSPGKIVRDINEKEREIIE
                     RTANNYVKYKENYKNSENFRSL"
     gene            complement(234..986)
                     /locus_tag="PK683_05425"
     CDS             complement(234..986)
                     /locus_tag="PK683_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNH07921.1"
                     /translation="MKAVQTILVGVLFLIASAVVGYFTIITEGGPLKKELHTMKIYFP
                     NSEGIKVGSKVTVQGVPFGYISRVSLAYFDAKGNLLESAQEGAETQVEVLIVLQSPVK
                     LYDNYEIKIRNESLLSGRIIAINPGFAKEDGVNVPAKRIHTVIDPAAVKPGKIKGKTS
                     EDPLVSLSELIAENRPDIRKTVSNIADITTKINSGKGTIGKLINNEDMHSNVNTTLTD
                     AQIVLRELREGLEDVREQAPVTSFIRAALTAF"
     gene            1201..1473
                     /locus_tag="PK683_05430"
     CDS             1201..1473
                     /locus_tag="PK683_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07922.1"
                     /translation="MVLVDKKTLIHWMLLDSLAKESYYREKIQIFKNKYKTDFPAFQN
                     RVESAEKESFEEWDDYIEWDAYEGFLSRLKERIHDIRNGNIQLAGI"
     gene            1538..1960
                     /locus_tag="PK683_05435"
     CDS             1538..1960
                     /locus_tag="PK683_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07923.1"
                     /translation="MDDDQAPAAVKKLADLFTKISEGIEKKQQDEFDAEIMKLYLHRE
                     SKVSISSDYNEKENSVGFSIFVSAPNSAVKPSEIHFNQFIYECLLLSDADQLKNIQEC
                     IELMERKDGSKYNLDFIPAYYNDELCEYYIHGRIRKKG"
     gene            1963..2238
                     /locus_tag="PK683_05440"
     CDS             1963..2238
                     /locus_tag="PK683_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07924.1"
                     /translation="MTEAKKGRCIKCNLFFYVHDHHILPKSRFGNEGETVRLCPNCHT
                     HFHEYSRINTRNPEDREEALKIWTIWLRTISVTVSIIIASIIINYLR"
BASE COUNT          951 a          594 c          602 g          825 t
ORIGIN      
        1 tggggaatat tcatttgtgg gtgcaggaac cctgatcact cccggaaaaa aaattcctcc
       61 cggagtgctt gtaatgggtt cccctggaaa gatcgtgcgt gatatcaatg aaaaagaaag
      121 agagatcatt gaaagaaccg caaacaacta tgtgaaatat aaagaaaatt ataaaaattc
      181 tgagaatttc agaagtctat agtcgtcagc agttaccttt tatatcaaac tgattaaaat
      241 gcagtgaggg cagcccggat gaaacttgtg acaggagcct gctctcttac atcctcaaga
      301 ccttccctga gttccctgag cacaatctga gcatcggtca gtgttgtgtt cacattgctg
      361 tgcatatcct cgttgttgat gagttttccg attgtgccct ttccgctgtt gatcttggtt
      421 gtgatgtccg caatattcga cacagtcttc cttatgtcgg gacggttttc tgcaatcagt
      481 tcggaaagac tgaccagagg atcttcactt gtctttccct tgatctttcc tggttttaca
      541 gcggccggat caatgactgt gtggattctt ttagcaggaa cgttgactcc gtcttctttg
      601 gcaaaacccg gattgatggc aatgattctt ccggaaagaa gactttcatt tcggatcttg
      661 atttcataat tatcatagag cttcacaggg gactgcagaa caatcagaac ttccacctga
      721 gtctctgcac cttcctgtgc tgattccaga agatttccct ttgcatcaaa ataggccaga
      781 ctgacccttg aaatataacc gaaaggcaca ccctgcactg tgaccttgga acctaccttg
      841 attccttccg aattgggaaa ataaatcttc attgtatgca gttccttttt cagcggcccg
      901 ccttcagtga tgatcgtaaa atatcctact acagcagaag cgattaaaaa gagcacccct
      961 accagaattg tctgaacagc tttcatttct cccccaagtc tcttccactc tcaaataaaa
     1021 ccggatacag tcaatgaatt tcagaggcgt aaggaatgcc cagcgattgt atatatgagt
     1081 aaaaaaatga tgctcacacg gagaagccga gtgtcagagc ggctcagatg cctgcaagat
     1141 cagcaatcac agcaataaaa atgcattgac aattgaaaaa aaagaaaaaa ccttgagcct
     1201 atggttcttg ttgacaaaaa aacgctgatt cactggatgc ttctggattc acttgcaaaa
     1261 gaaagctatt atagagaaaa aattcagata ttcaagaaca aatacaaaac cgattttcct
     1321 gcatttcaaa accgagtgga atcagcagaa aaggaatcat tcgaagaatg ggatgattat
     1381 atagaatggg atgcttatga aggtttttta agccgcctga aggaacgaat tcatgacatc
     1441 cgaaacggaa atattcaatt ggctggaatt tgaaagatca aaaaatggac aggaaaacag
     1501 cctgtaaact ggcatggaac ctatgactgc gagaactatg gacgacgatc aagcacctgc
     1561 tgctgtgaaa aagcttgcag atttattcac aaaaatatct gaaggcattg aaaaaaaaca
     1621 gcaggatgaa tttgatgcag aaatcatgaa attatatctg cacagagaaa gtaaagtttc
     1681 catctcttct gactataacg aaaaagaaaa ttcagtggga ttcagcatat ttgtgtctgc
     1741 cccgaactct gctgtcaaac cgtctgaaat tcatttcaat cagtttatct atgaatgcct
     1801 gcttctttct gatgcggatc agctgaaaaa tattcaggaa tgcattgaac tgatggaacg
     1861 gaaggacggc agcaagtata atctggattt cattcctgcg tattataatg atgaattatg
     1921 tgaatactat atacatggaa ggatacgaaa aaagggataa gaatgacgga agcaaaaaaa
     1981 ggacgatgca taaaatgcaa tctgtttttt tatgtacatg accatcatat tctcccaaag
     2041 agccgattcg gcaatgaagg agaaacagtc agactctgcc ccaactgtca tacccatttt
     2101 catgaataca gcagaataaa tacaagaaat cctgaagaca gggaagaagc attaaaaatc
     2161 tggactatat ggttaagaac aatatcagta acggtaagta ttattattgc aagcattata
     2221 attaattatt taagatagat aaacagtcac atcacatcga ggcctgacaa tactgataac
     2281 tctctgctaa atcatcaatc gcctcagatt caagccttaa ttttctttcc aaatctctcc
     2341 tccaggatct gaatggattc ttcccaggaa tacccgcctc ctgcattctt tttaaacgga
     2401 tatcctgttc ctttttataa gcctgatcca tttcctctgt caggtcggaa tcgtcaccct
     2461 cttcataaat gacaattgcc agtttatggt agggttttat ttacggaggg agttccaaag
     2521 cagcaaatcg agtctcagtg gtatctgcaa tcgtttccaa agcagtcata tttcaaatga
     2581 taaatactca gatttccaat gaaattttaa tccggtcaat tattcattga ttgaaagtgc
     2641 ctttgcattt tcactgaacg tcgccttgca ggctagcaaa tgcggggatt ttagcggctt
     2701 caatgttagc gaaggggctg cttaagcaca tttaaaccaa cgcaagttgg gaagatgctg
     2761 ccggtaaagc atccctgcct ttctatattt ctaatagccc gcagggcgga gcgcagtcga
     2821 gattagaaat ctgagtaaat ggaatatatc agaaaacagg caaatatttt attgaaagcg
     2881 aagatgtgac tgaaatacta gaaactgcga tgatttccga atgcagtatt ttaaagagtg
     2941 attctgtaat gatttatgat gggcagctct ta
//
LOCUS       DAOQGJ010000266         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20036_length_2968_cov_2.431171, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000266 DAOQGJ010000000
VERSION     DAOQGJ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20036_length_2968_cov_2.431171"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PK683_05445"
     CDS             <1..690
                     /locus_tag="PK683_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNH07925.1"
                     /translation="HNKELAAMFEKYLKYEFELRSFKQDPNLKFEDRYEQIKKKRRDI
                     FKDEEARLIFGLEEAKVNFLQANSSFLQSSKNLSGEDRIKAYEKMREQVYGPYYKTVT
                     EREPKFTKYETEVSLRENDFRKLSDSEKTAKLESVQEKYFGKDGVERIRKVKAELEEE
                     NKRISIFQEKEKKFLSANSSLNEKEKLEKLDKLRLEVFGSQEAADSYRRQQEFIEYLK
                     GEEARLKKKGK"
     gene            687..2336
                     /locus_tag="PK683_05450"
     CDS             687..2336
                     /locus_tag="PK683_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNH07926.1"
                     /translation="MKEYFFLFLWAAGMFFSLGPFEYSGAGFLSAVFLVSAVQNLEKH
                     SYYRVLALSAAASFFCTFICFPWITSGLANLSGIRIEFSFLIVLIFSFVMQFKMYAFF
                     ISCRILFPYFPENHRVWVVGLAAFTVDKYVFQIFPWHFGDMSAGSSLFRELAFYFGVN
                     GISFFIFFPASFTVFLFRKYIQKTEISSLKELWPISAVLMTITVLIFFKKSVPQGISG
                     RLLALALQTNTELGLKSGSMEASAGKALNESFSLALKALYSGQYVPDLIIFPETAVPF
                     LNTEPFQRNYSPTFHGIILYLSSLSGADIAWNEPYAEKNGTVNSLAHFSHHSKTKQRY
                     EKNLLVPFGESLPLSESFPFLNSLFPESSKYVRSENRIMPKIFYKKSYKEKDYINLNE
                     INAEMLQRPSEILNYVPRREDYAETDYIPLLCYESLFPDYVRSFTEKSQPGFLLSLAN
                     DSWFGNYLENWQHETGARFRAVESGRAFIRVTLTGVTSVILPDGSSVHPLGSAKQDFG
                     FYEIPLYTGKTVFYRFPAVMEIICCIILVFAFRNRLMKAKH"
     gene            2419..>2968
                     /locus_tag="PK683_05455"
     CDS             2419..>2968
                     /locus_tag="PK683_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNH07927.1"
                     /translation="MKVESFYKFFLITPSSYLPVINSENLLYGLLSKKKITEEMADLD
                     LSGIEYETVPEHFLEKEMNESLLYYFHSHKTIPVLNENGQRIDNWDKTRFLSEFSRMT
                     DMPKTEKIPEKEKLPESEPTSEIPANRLMIFKFMELILRNFPDALFAADREGNTTFYN
                     EKFETDVLSKSIFRDSIGFAEKY"
BASE COUNT          890 a          591 c          603 g          884 t
ORIGIN      
        1 cataataagg aacttgctgc catgtttgaa aaatatctta aatatgaatt tgaattaaga
       61 tcattcaagc aggaccctaa tctcaaattt gaagacaggt atgagcagat taagaaaaag
      121 agaagagaca ttttcaagga tgaagaagcc agactgatct tcggtctcga ggaagcaaaa
      181 gtcaactttc ttcaggcaaa cagcagtttt ctgcagagtt caaaaaatct ttccggtgaa
      241 gacagaatta aggcatacga aaaaatgaga gagcaggtgt acggacctta ctataagaca
      301 gtaacggaac gggagcctaa gtttaccaaa tatgaaactg aagtctcctt aagagaaaat
      361 gatttcagaa aactttccga ttctgagaag actgcgaagc tggaatcggt tcaggaaaag
      421 tattttggaa aagacggagt ggaaagaata aggaaagtca aagctgaact tgaagaggaa
      481 aataaaagga tatccatatt tcaggaaaaa gaaaagaagt ttctctctgc aaattcctct
      541 ttaaatgaaa aggaaaagct cgaaaaactg gacaagctca gacttgaagt gttcggatct
      601 caggaagcgg ctgattctta cagaaggcag caggaattta ttgaatatct caaaggtgaa
      661 gaagcaagac tgaaaaagaa ggggaagtga aagaatactt ttttctcttt ctgtgggcag
      721 cgggaatgtt tttctcgctc ggcccgttcg agtattccgg tgcaggcttc ctgtctgcgg
      781 tatttcttgt atcggctgtt cagaatctgg aaaagcactc ctattaccgt gtgcttgctc
      841 tgtctgctgc agcttctttt ttctgtacat ttatctgttt tccctggata acctccggtc
      901 tggccaatct ttccggaatc aggattgagt tttcattcct gatcgttcta attttttcat
      961 ttgtaatgca gtttaaaatg tatgcttttt ttatttcctg cagaattctt ttcccttatt
     1021 ttccggaaaa ccatagagtc tgggtcgttg gtcttgctgc atttactgtc gacaaatatg
     1081 tgttccagat ttttccctgg catttcggag atatgagtgc cggaagcagt ctcttcagag
     1141 aacttgcatt ttatttcgga gtaaacggaa tttccttttt cattttcttt ccagcatcct
     1201 ttactgtgtt tcttttcaga aaatacattc agaaaactga gatctcctca ttgaaggaac
     1261 tttggcctat ctctgcagta ttaatgacta taactgttct gatatttttt aagaaatcgg
     1321 taccccaggg aatatccgga agactcctcg cccttgcact gcagacaaat acagaacttg
     1381 gtctgaagtc aggcagtatg gaggcctcag ccggaaaagc attgaacgaa tcgttctcac
     1441 ttgccttaaa ggcactctac tcaggacagt atgtccccga cctgatcatt tttcctgaaa
     1501 cagccgttcc ttttctgaat acagagccgt ttcagagaaa ctattctccc acttttcacg
     1561 gaattatttt atatctttct tccctttccg gtgcagatat tgcctggaat gaaccttatg
     1621 cagaaaagaa cggaacagtc aattctctcg cgcatttttc tcatcattcc aagacaaagc
     1681 agagatatga aaaaaatctc cttgttccgt tcggggaaag tcttcctctc agtgaatctt
     1741 ttccctttct gaacagtctg tttccggaaa gttccaaata tgtgagatcg gaaaacagaa
     1801 ttatgccgaa gattttttat aaaaaaagtt ataaagaaaa ggactatata aatctaaacg
     1861 aaattaatgc tgaaatgctt cagagaccct cggagattct gaactatgtt ccccgcagag
     1921 aggattatgc tgaaactgac tatatcccgc ttctatgtta cgaaagtctt tttccagatt
     1981 atgtccgaag ttttacagag aagagtcagc ccggtttttt acttagtctg gcaaatgatt
     2041 cctggtttgg aaactacctt gaaaactggc agcatgagac cggtgctcgg ttcagggcag
     2101 ttgaatcagg cagagcattc attcgggtta ctctcaccgg tgtgacttca gtcatccttc
     2161 ctgacggaag ttctgttcat cctctcggat cagcaaaaca ggacttcggg ttttatgaga
     2221 ttcctctcta cacaggaaag actgtgttct accgtttccc tgcagttatg gaaattatat
     2281 gctgcatcat tcttgtattt gccttccgta accggcttat gaaagcaaag cattgatttt
     2341 ttgaccgaaa cttcagcagg agccaaaaat acttctcgac ttggaaatta atggattaaa
     2401 actgacctta agccattcat gaaagtcgaa tctttctaca agttttttct gattacaccc
     2461 tcgtcatatc tccctgtaat caattctgaa aatcttttgt acggacttct gtccaaaaaa
     2521 aagattacag aagaaatggc ggatcttgac ctttccggga ttgaatatga aacggttccc
     2581 gaacatttcc tcgaaaaaga aatgaatgaa agtctcctct attatttcca ttctcataaa
     2641 acaattccgg tcctgaatga aaatggtcag agaatagaca attgggacaa gacaagattc
     2701 ctttctgaat tttcccgaat gacagatatg cctaagactg agaagattcc ggagaaggaa
     2761 aaactgcctg aatctgaacc gacctctgaa attcctgcaa acaggctgat gatctttaaa
     2821 tttatggagc ttattctgag gaacttccct gatgcacttt tcgcggcgga cagggaaggg
     2881 aacaccacct tttataatga aaagtttgaa actgatgtgc tctccaagag cattttcagg
     2941 gactctatcg gttttgcaga gaaatatt
//
LOCUS       DAOQGJ010000267         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20120_length_2962_cov_2.488476, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000267 DAOQGJ010000000
VERSION     DAOQGJ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20120_length_2962_cov_2.488476"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PK683_05460"
     CDS             complement(<1..577)
                     /locus_tag="PK683_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH07928.1"
                     /translation="MKNGLDFWKKTVKNFMMGRNTFTFFLLFIFSCDSKYSSFTDLEY
                     AVSKGDFPRLQLLQKRGADLKLKDRKTGKFLLHYGAENNQSEIIEFLLKEGLETDLPD
                     KEGRTPLFYAMKDSHIRTQEILYGRGSDLHRKTKSGSALLHYAAMNGAAKTIESLIRK
                     KMRVDEKDSAGNTALHTAAMHHQQTSAEMLVR"
     gene            665..1399
                     /locus_tag="PK683_05465"
     CDS             665..1399
                     /locus_tag="PK683_05465"
                     /inference="COORDINATES: protein motif:HMM:NF025021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH07929.1"
                     /translation="MFSAYQSYRLSSVRFFYGAISLLLISSCISLHDPVMNGDMNSIK
                     YMSEHGYSEDEQNEAGETPIFIAAWRGDANIVKYLIKRGASLNWQDREGWTALHWAAG
                     SGNQEIVRILIENGAKVNVRNKYHYTPLHGAASLEGSDTVKYLTEHGADVNVKNLYGW
                     TALQCASAFGRKETAEYLIAHGADPEIRNDLEKSVQVGNRNTADKQEESGLKSAGRTF
                     LVFLEAVGFIIQVGQFIAFIGSVLHH"
     gene            1405..1638
                     /locus_tag="PK683_05470"
     CDS             1405..1638
                     /locus_tag="PK683_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07930.1"
                     /translation="MEKEEEKFHKQLLRKGEFDRPEAAVEYARWLLEQGRYEHAELVI
                     YTALDEGPRFDLYEMRAMSALLNLENFGEKSAE"
     gene            2299..>2962
                     /locus_tag="PK683_05475"
     CDS             2299..>2962
                     /locus_tag="PK683_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNH07931.1"
                     /translation="MGRKFHQDTIALHGGQSADPTTTSRAVPIYQTTSYVFKDTDHAA
                     RLFGLQEFGNIYTRLMNPTTDVLENRVAQLEGGVAALATASGQSAETLAILNIVESGY
                     EVVSSSSLYGGTYNLFHYTFPKLGIKVHFVDPSNPENFRKAVNDKTRAFYCETLGNPK
                     LDTLDLEAIAKVAHESGVPFIVDNTLASPYLVNPIEHGADIVVHSLTKFLGGHGTSIG
                     GII"
BASE COUNT          864 a          595 c          654 g          849 t
ORIGIN      
        1 accggacaag catttcagct gaagtctgct gatggtgcat agctgctgta tgaagcgctg
       61 tatttcctgc tgaatccttt tcatctacac gcatcttttt tctgatcaga gattcaattg
      121 tctttgctgc tccgttcatc gcagcataat gaagcagagc agagcctgat tttgtctttc
      181 ggtgcaagtc agatcctctt ccatacagaa tttcctgtgt cctgatatgc gaatctttca
      241 ttgcataaaa aagaggagtt cttccttcct tgtccgggag atcagtttcc agaccttctt
      301 tcaaaagaaa ttctataatc tcagactgat tgttttctgc accatagtga agcagaaatt
      361 ttcctgtttt tctgtctttc agtttaaggt ctgcgcctct tttttgaagg agctgcagcc
      421 gaggaaagtc accttttgat acagcatatt ccagatctgt gaatgaagaa tattttgaat
      481 cacagctgaa aatgaaaagc agaaagaagg tgaaagtatt acggcccatc ataaaatttt
      541 ttacagtttt tttccaaaag tcaagcccat ttttcactgc tgagggctgc tgtattttct
      601 ttaatgaaga gcgaatcaaa aaaagattga cattctgact ctatcgtgaa aattttcacg
      661 ttatatgttt tctgcatatc agtcataccg tttgtcttca gtcagatttt tttatggagc
      721 gatttctctt ttgctgatct cgtcctgcat aagccttcac gatccggtaa tgaatggaga
      781 tatgaactca atcaaatata tgtcggagca cgggtacagt gaagatgagc aaaatgaagc
      841 cggtgaaacg cccatattca ttgcagcctg gagaggtgat gcgaatatag taaaatatct
      901 tattaaacgg ggtgcttcgc tcaactggca ggacagagaa ggctggactg cgcttcactg
      961 ggcggccggt tcaggaaatc aggagattgt aagaatactg attgaaaacg gcgcaaaagt
     1021 caatgtcagg aacaagtatc attatactcc tctgcacgga gctgcttcac tggaaggatc
     1081 tgatacagta aaatatctta cagaacacgg tgcagatgta aatgtaaaaa atttgtacgg
     1141 atggactgct ttacagtgtg catctgcatt cggaagaaaa gagactgctg aatacctgat
     1201 tgcccacggt gccgatcctg aaatcagaaa tgacttggaa aaatctgtgc aggtcggaaa
     1261 tcggaataca gcagataaac aggaagaatc cggtctgaaa tcggcaggca gaacattcct
     1321 tgtgttcctt gaggcagtgg gatttattat tcaggtcggt cagtttattg catttatcgg
     1381 atcagttctg catcattaaa aaatatggaa aaagaagaag aaaaatttca taagcagctt
     1441 ctccgaaaag gagagtttga cagaccggaa gcggctgtag aatatgcccg ttggctgctg
     1501 gaacagggac gttatgagca tgcagaactg gtcatttaca ctgcactgga tgagggcccc
     1561 cggtttgatc tgtatgagat gcgcgcaatg tctgcccttc tgaatcttga gaactttgga
     1621 gagaagtccg cagaatgaca ggtgcggaaa atacagatct tccactgata tctgcaatga
     1681 ggggctcggc aaaaaagacc ctgactggaa agctgttctt gataagataa agaccaatct
     1741 gaaccgctgc agatagcttc tgaaatattt cataatttta atgaaatttt cattattact
     1801 gaatgactcc ggacaggctg acagaagcac ggacagcggt tttaattcct gcgaggatat
     1861 atttttttat aatgataata aagtttcaat ctccatccgc agaacctcaa atgcagacag
     1921 ttcggtgcat cagtgattct gcgcaggtca atttttgagt cggaagacag gaaagtcatc
     1981 ctctcgaatt cttatacttc tattcaaaag tctttacata tttcaacctt gttttgcctg
     2041 taaacaaaag gtctcccggc acaaaaaaac acaattgctt ttgtctgtca gtataattgg
     2101 aagatggaat ctgcattggg tcctgtcagt cggttctgat aatgcagccg caggcggatg
     2161 ttctaatata gcaaacgttt tttccaattt attggaatct tggttgacct gaaaaggggc
     2221 attgtcagca tagatttaat cagaagattc cggcgccatt ttgcagaccg gttcaaaact
     2281 tttacaggag taaattgaat gggaagaaaa tttcatcagg ataccatagc tctgcacgga
     2341 ggacaaagtg cagatccaac tacaacttcg agagcagttc ctatatatca gacaacttcg
     2401 tacgtattca aggacactga tcatgcagcc agactgttcg gtcttcagga attcggaaat
     2461 atctatacaa gactgatgaa tcctactaca gatgttcttg agaacagagt tgcacagctt
     2521 gaaggcggag tcgctgctct ggccaccgct tcaggtcagt ctgctgaaac tcttgccatt
     2581 ctgaacattg tggaatccgg atatgaagta gtttcttctt cttcgctgta cggcggaaca
     2641 tacaatcttt ttcattatac cttccctaag cttggaataa aggttcattt tgtcgaccct
     2701 tccaatcctg aaaatttcag gaaggcagta aacgacaaaa caagagcatt ctactgtgaa
     2761 actctgggaa atccaaaact ggatacactg gatctggaag ctatagcaaa ggttgcacat
     2821 gaatcaggtg ttcctttcat cgtggacaat actcttgcat ctccttacct tgtgaatccg
     2881 attgagcacg gagctgacat agtggtgcat tcccttacca aatttctggg aggacacgga
     2941 acttccatcg gcgggatcat ta
//
LOCUS       DAOQGJ010000268         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20165_length_2958_cov_2.857044, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000268 DAOQGJ010000000
VERSION     DAOQGJ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20165_length_2958_cov_2.857044"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PK683_05480"
     CDS             complement(<1..1094)
                     /locus_tag="PK683_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HNH07932.1"
                     /translation="MKKSIRKAFSILAVLTVSVLSLNFGVGVLDGGLPQPLPKVSADA
                     SGNAVFSFPIEVFPGTKGVDPELGASYSSAAGDGDFGAGWNLSGIGRITRSPAGMKYT
                     ADDAFISSVSGKLVRTGTSPNGFYYRNDPDAHFIYEYNVNTNSWSAKDSAGNLYLFNE
                     VSYFGSSVKSWFLTEFRDSSGNSWKVSYSDRSRLDEDDVRISSIVYADRTVTFTYADS
                     SLYSTLFTYGNAERDRSLISSIKVEAGSRYREYTFTYEKGEFGKERLKKIGRKENTGN
                     GEAVFRDIEFSYNADRTLDMEQQRDYRINNTYAFSQIPFQDKTQCVSGELACQQAGVS
                     CAPGASYPACASTKAQNAAKCTTYKQRWQAV"
     gene            complement(1120..1395)
                     /locus_tag="PK683_05485"
     CDS             complement(1120..1395)
                     /locus_tag="PK683_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07933.1"
                     /translation="MKKTLFYITVLAGFLSAGCGGTVSQSEVQARADRCSKILKDAGS
                     YSSFAARYSGNCYTCTPYPDLTPFIIAYTACRKSLNEMKSGRTEIPL"
     gene            complement(1506..2207)
                     /locus_tag="PK683_05490"
     CDS             complement(1506..2207)
                     /locus_tag="PK683_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNH07934.1"
                     /translation="MISFIGERRKRIKRPPGILFAGIMCFLYPVINIWSLCTFYHIPV
                     RDFAQVFRFINGFQTALVFAPALPGIGLLSVKKWGWYSFLAYCFSLLLFNIYALYKFH
                     ASRNVYSILETAAVIAVSVYFLRRDISAPYMKLYPRGFRGENRIPVEAEVEILCGSEK
                     WTRKTRDFSVSGFYTDWNDFPLHLNSEILARFRLGNESFELNAGVVRTDENGAGFAFR
                     DTDSETESRLERSLA"
     gene            complement(2204..>2958)
                     /locus_tag="PK683_05495"
     CDS             complement(2204..>2958)
                     /locus_tag="PK683_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH07935.1"
                     /translation="SEGDSVMIKYIRKMKNIFMDLFIPIPCSSLNSEHQALNSCFKTL
                     NPKPRTSSSFFNDPRSVFPFLLCVILLFSAPVFSQSQNYGIKYPEIKTLILENRFDAA
                     QKLLDASAGTETGSASLELYQTEIWIGRANSLYESGKKKSAFEYYELAYQKWPNHPTV
                     YQRYNELRGRSLSDLSVRNTAGHYGAGNAVSISEKAYDRRAADSAVQPERRTEKEDTF
                     LFAWFLVLSALVLSLLATNISLLIILARQEKK"
BASE COUNT          752 a          710 c          650 g          846 t
ORIGIN      
        1 accgcctgcc atctctgttt gtaagtagtg cactttgctg cgttctgagc tttcgtagag
       61 gcacacgcag gatatgatgc tccgggcgca cagctgactc ctgcctgctg gcaggccagt
      121 tctccgctga cgcactgagt cttgtcctga aacggaatct gggaaaaggc gtaggtattg
      181 tttatgcggt agtctctctg ctgctccata tccagtgtcc tgtcggcatt gtagctgaat
      241 tcaatgtctc tgaagactgc ttccccgttc cctgtattct cctttctgcc gatctttttc
      301 agtctttcct tcccgaactc ccctttttca taagtgaacg tatattcacg gtatctgctg
      361 cctgcctcca cttttattga agaaatcagc gatctgtctc tttctgcatt tccgtacgtg
      421 aacagggtcg aatacaggga agagtctgca taagtgaaag tgacagttct gtctgcatag
      481 acaattgaac tgatcctcac atcgtcttca tccagtctgc ttctgtcgga ataggacact
      541 ttccacgaat tcccggaact gtcccgaaac tcagtcagaa accatgactt cacactgctt
      601 ccgaaataag acacttcatt gaacagataa agatttcctg cgctgtcctt cgcagaccat
      661 gaattcgtat ttacattgta ttcgtaaatg aaatgagcat caggatcatt tctgtaataa
      721 aaaccgttcg gacttgttcc ggttctgacg agtttcccgg agacagaact gatgaaagca
      781 tcatctgcag tgtatttcat cccggcaggc gatcttgtaa tcctgccgat tcctgacaga
      841 ttccatcctg caccgaaatc tccgtctcct gctgcagaac tgtatgatgc gcccagctcc
      901 ggatcgacac cctttgtccc tggaaaaact tcaatcggaa aagaaaatac cgcattgcct
      961 gatgcatctg ccgaaacttt cggaagaggc tgaggaagtc ctccgtcaag caccccgacc
     1021 ccgaaattca gtgaaagaac ggagacggtg agaactgcca gtatggaaaa cgcttttctg
     1081 attgattttt tcattttatt ttttctcctt ttgttcctgt cagagaggaa tctctgtcct
     1141 gcccgatttc atctcattca ggctttttct gcaggcagta tacgctatga tgaacggggt
     1201 cagatcagga tacggagtgc atgtatagca gtttcctgaa tatcttgctg caaaactgga
     1261 ataggaacct gcgtccttca gtatcttgct gcatctgtcc gcccgggcct gaacttccga
     1321 ctgactgact gtcccgccgc atccggctga aagaaagcct gcaagaactg ttatgtaaaa
     1381 taatgttttt ttcatgatta tctcctcaga tttagtaatc accttacgca ggtggggcgt
     1441 tatatagggg agcctaccgg cggttttaag ggcttcgccc ttaaaaatcc catttttttc
     1501 tctcctcatg caagactcct ttccagtctg ctttcagttt cactgtcagt gtcacggaac
     1561 gcgaatcctg caccgttttc atctgtccgc acaacccctg cattcagttc aaaagattca
     1621 tttcccagcc tgaaccttgc aagaatctct gaattcagat gaagaggaaa atcattccag
     1681 tccgtataaa agccggatac ggaaaaatcc ctggtcttcc ttgtccattt ttcactgccg
     1741 cagagtatct cgacttctgc ttccaccgga attctatttt ctccccggaa tcccctggga
     1801 tacagtttca tgtaaggagc ggatatgtct cttctgagaa aatagactga cactgcaatc
     1861 actgcagcag tttcaagaat ggaatagaca tttctcgatg catgaaattt atataaggca
     1921 tatatattga acagaagaag cgagaagcag tatgcaagaa aactgtacca gccccatttc
     1981 ttcactgaaa gaagtccgat cccaggcagt gccggagcaa agacaagggc tgtctgaaat
     2041 ccgttgatga acctgaaaac ctgggcgaag tccctgaccg ggatatgata aaaggtgcat
     2101 agactccaga tgtttatgac aggataaagg aagcacatga tgcctgcaaa aagaatgccg
     2161 ggaggtcttt taattctttt tctccgttcg ccgatgaatg aaatcatttt ttttcctgcc
     2221 ttgccagtat gatgagtaag ctgatgtttg tcgcaagtag ggaaagaacg agtgcagaaa
     2281 gcacaagaaa ccaggcaaaa agaaatgtgt cttccttctc tgttcttctt tccggctgca
     2341 ctgctgaatc cgcagccctt ctgtcgtatg ctttttcgga gatgctgact gcatttcctg
     2401 ctccatagtg tccggcagtg tttctgaccg acagatccga aagactcctt cctctcagtt
     2461 cattgtatct ctgatacact gtcggatgat tgggccactt ctggtatgca agttcataat
     2521 actcgaaagc agattttttc tttccggact cataaagact gttcgctctc cctatccaga
     2581 tttctgtctg gtaaagttca agactggcac tgcctgtctc tgttcctgcc gatgcatcga
     2641 gaagcttctg agcggcgtca aacctgtttt caagaatcag tgtctttatt tcaggatatt
     2701 ttatgccgta attctgagat tgggaaaaga ctggggcaga gaaaagaagg atgacacaga
     2761 ggagaaaagg gaatacggat cgagggtcat tgaagaaaga gcttgaagtt cggggcttgg
     2821 ggttcagggt tttgaaacac gagttcagag cctggtgttc ggagttcagg gaactgcagg
     2881 gaatagggat aaataaatcc atgaaaatat ttttcatctt cctaatatac tttatcatta
     2941 cagaatcccc ttccgaac
//
LOCUS       DAOQGJ010000269         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20186_length_2956_cov_2.841089, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000269 DAOQGJ010000000
VERSION     DAOQGJ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20186_length_2956_cov_2.841089"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2956)
                     /locus_tag="PK683_05500"
     CDS             complement(<1..>2956)
                     /locus_tag="PK683_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH07936.1"
                     /translation="AHDRIQQAVYSLIPESERKKIHLKTGKLLFENSNEKERQDRLFD
                     ITNQLNAADDLITDETEKFQLFCLNLSSAIKSKSSTAYTSALSYIGCGYRHYNDDFWN
                     IHYDLCAEFFRVWAEAAYLNSDLEKSVEIISFALGKVKTAADKGELYNLLTVEYTLSA
                     KYGDALETGADALRILGVDLPASDYENALKAEMAKVAAALSGRKIGSLIDLPLMESKE
                     KTVAMKLLNYMVPPAFMTNQPLFLVIAAKMVNISLEFGNTGESSYGYAVYGIVLCQVL
                     LDYKNGYEFGSLAYELSLKLKNEVKKCLACEVLVGHLNHWRKPLKQSQDLTEIGFKAG
                     VNSGEIQFAGYIYLYQAYNRYYEGVNLRQYNEEMEKYLEFTDRTKNTHASDVILGSQL
                     TVSLLSRIKTKSGDYDEASHFLRAKERQSNLFICPYYVMKSQIHFLFQEYEKGLEYAV
                     LAEERSSDVPGVISTAENKFYYALNIIGIMKSGRELNDRDFEKLFSDLERNKLSLKYL
                     SEISPENFLHKVILLDAEKAALEGRNFEAMKLYETAIESAWENGFTQNEAMASELAGM
                     FYLKNGFEDIASLYLMRARSGYLSWGAISKLEKFDRDYSQHLKTQKAVKSVTSSSSNT
                     FDTRFTSSVQLDTGSILKASRSISGEIVLEKLLKKMMEIISENAGAEKAVLLLPKKDK
                     WHIEAESSINAGKIRILESIPLSEYGEIPVSLIQYSVRTRENLVISNASRDPRFSNDE
                     YIIKNEPYSIMCIPLIHQGSLKGIIYLENRLAAGAFSLERVQMLQMFAAQISISIENA
                     QLYKSLEEKVEERTKDLKLAQDELVQSEKMAALGQLVAGVAHEINNPIGAIISSIESI
                     SGFFKNKFFAISEAMKDMDSEELEIFRKIVLEFLEKTPDFLTSKEKRQIRKETAADLE
                     SKGFEDAYTLADYITDIRMENRIEEIYPLIKSGSPDRLRKLYEIFSMIRGISNIQISA
                     SKTAKIVYALKSY"
BASE COUNT          769 a          647 c          576 g          964 t
ORIGIN      
        1 gtaagacttc agcgcataaa ctattttggc cgtcttggaa gccgatatct gtatattgct
       61 gatacctctt atcatggaaa atatttcata aagttttctg agtctgtcag gacttcctga
      121 ttttatcaaa ggataaattt cttcaattcg attttccatc cgtatgtctg ttatataatc
      181 agcaagtgta taagcatctt cgaaaccctt tgattccaga tctgccgctg tttcctttct
      241 gatctgtctc ttttccttgg atgtgagaaa atcaggagtc ttttccagga attcgagcac
      301 aatttttcgg aagatctcca gctcttcaga atccatatcc ttcattgctt cggaaatggc
      361 aaagaacttg ttcttgaaaa accctgaaat gctttcaatc gaggaaataa ttgcaccgat
      421 tggattgttg atttcatggg cgactcccgc caccagctgt ccaagagccg ccatcttttc
      481 tgactgaaca agttcatcct gtgcaagttt aagatccttt gttctctctt cgactttttc
      541 ttccagactc ttatagagct gagcattttc tatggaaatg gaaatctgag ctgcaaacat
      601 ctgaagcatc tgaactcttt ccagtgaaaa agctccggca gcaagccggt tttccagata
      661 tataattcct ttcagactcc cctgatgaat caaaggtatg cacataatgg aatatggttc
      721 atttttaatt atatattcat cattggaaaa acgaggatca cgggaagcat tgctgatgac
      781 aagattttcc ctggttctaa ctgaatactg aatcagagat actggaattt ctccgtattc
      841 ggaaagaggt atggattcga ggattctgat ctttcctgca tttatgctgg actcagcttc
      901 aatatgccat ttgtcctttt ttggaagaag cagtacagcc ttttctgcac cggcattttc
      961 gctgatgatc tccatcattt ttttcaggag tttctcgagt acaatttcgc ctgaaatgct
     1021 tctggaggcc ttcaggatgg atccggtgtc cagctgaact gaagaggtga aacgggtgtc
     1081 gaatgtattg gatgagctgg atgtgactga tttcacggcc ttctgtgtct tcagatgctg
     1141 agagtaatct ctgtcaaact tttccagctt ggatatggct ccccaggaaa gataaccgct
     1201 tcttgcccgc attaaatata atgatgctat atcctcaaat ccatttttca gataaaacat
     1261 tcctgcgagc tcggaagcca tagcttcatt ctgagtaaac ccgttttccc aggcggattc
     1321 tattgcagtt tcataaagtt tcattgcttc aaaatttctc ccttccagtg cagctttttc
     1381 tgcgtccagc aggattacct tgtgcagaaa gttttcgggg gatatttcgg aaagatattt
     1441 taatgataat ttattccgtt caaggtctga aaacagtttt tcaaaatctc tgtcattcag
     1501 ttccctgcct gatttcatta ttcctattat attgagtgca tagtaaaatt tgttctctgc
     1561 cgtagatatg acacccggta cgtccgacga tctttcctct gccaggacgg cgtattccag
     1621 acctttttca tattcctgaa aaaggaaatg tatctgcgat ttcattacat agtatgggca
     1681 tatgaaaaga ttgctctgtc tctcttttgc tctaagaaag tgagatgcct catcataatc
     1741 tccggacttt gtcttgattc tgctgagaag agacactgtc agctgcgaac ccagaatgac
     1801 atctgaggca tgagtgttct tggttctgtc tgtaaattca agatattttt ccatctcttc
     1861 attgtactgc ctcaggttca ctccttcata gtaccggttg taagcctggt agagataaat
     1921 gtatcctgca aactgaatct ctcctgaatt tactcccgct ttgaatccga tttccgtcag
     1981 atcctgactc tgcttcaaag gttttctcca gtgattcaga tgccctacca gaacttcaca
     2041 ggcaaggcat tttttaactt catttttcag cttcagactg agttcatatg ccagagatcc
     2101 aaattcataa ccatttttat aatcaagaag cacctgacac agcacaatcc cgtaaactgc
     2161 ataaccgtag gatgactctc ctgtatttcc gaattccagt gaaatattga ccatctttgc
     2221 agcgattaca agaaacaggg gctgatttgt cataaatgca ggcggtacca tatagttcag
     2281 aagcttcatt gcgactgtct tctcttttga ttccatcaaa ggaagatcaa tcagtgatcc
     2341 tatctttcgt ccggaaagag cagcggcgac ttttgccatt tctgccttaa gtgcattttc
     2401 atagtcggaa gccggcagat ctacaccgag aatccgaagt gcatccgcac cggtctcaag
     2461 tgcgtctccg tactttgcag agagggtgta ctcaactgta agaagattat aaagttcccc
     2521 tttgtctgct gcagtcttta cttttcccag agcaaaactg atgatttcta cagatttttc
     2581 cagatctgag ttcagatagg ccgcttccgc ccataccctg aagaattcag cacagagatc
     2641 ataatgaata ttccaaaaat catcattata atgtctgtat ccgcatccta tgtaactgag
     2701 agcagatgtg taagctgtag atgattttga ttttattgca gaagagaggt tgagacagaa
     2761 aagctgaaat ttttcagttt catccgttat caggtcatcc gcagcattga gctgattcgt
     2821 aatgtcaaac agtctgtcct gcctttcctt ttcattcgag ttttcgaaaa gaagttttcc
     2881 tgtcttcaga tgaatttttt ttctttcgct ttccgggata agcgaataga cggcctgctg
     2941 gattctgtca tgtgca
//
LOCUS       DAOQGJ010000270         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20206_length_2954_cov_3.777165, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000270 DAOQGJ010000000
VERSION     DAOQGJ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20206_length_2954_cov_3.777165"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="PK683_05505"
     CDS             <1..1323
                     /locus_tag="PK683_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH07937.1"
                     /translation="AVVNDSSAVFYNPAGLGRNNFVQNYIELQKSKGPAVIMPFITTQ
                     ESLKKKTPGKKTEDEKKNEPASEKTGKGECIDVPVKKGFLNEIHIMGSLTQPLLKASG
                     GQSEDIKRVKDSYAGLSLSIDLNNFIKLNRNARFGLNILLPATGNLLSINDLNPGSPR
                     YLQNGHSLERPTIMGGAGIELWKDRLYAGVGFTALVKGRGSALLKNVQLSPDQTVPNQ
                     QVIVDIKPFVNPTFGLMGQWKNFYTGISYKREIMTSVDPVNARAQTAILAIQLDVKLA
                     LFDHFTPRTWNYGIGYRFLQKYLVTADLNRELWSAFKMSSVKNEYSQPVFFRDISNPR
                     VGFEYSFRDNILFRAGYGKRMNPAGRMPGEANIIDFERRIGSIGATYAFFPDPDSIIN
                     TPVILDLVYEYHSLKSEVQNKYMHSADSANYTAKGRIHHFGFSLTAYF"
     gene            1438..2697
                     /locus_tag="PK683_05510"
     CDS             1438..2697
                     /locus_tag="PK683_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNH07938.1"
                     /translation="MINRKCITEFFSSASGYEEYSQEEKIYETFAGYTGFLLSVILAF
                     SVCTEKRSGRLQTLGKDEGKVIIAGWPGYVEKGENDSKYDWVSEFEKNNHCKVENIIF
                     KNEHDISRVLQDGTADLVILSSELVLKYARDEKLREKNLDLLPNWANVDSRVRNSSWF
                     KILDKSYGVPIGWSANYLLFNKNYFSVEPDSWELMFEEGKLSDGRSSAKRIQAYGNPV
                     YIADAALYLKYKRPELKIQNIMNLNEVQYKAVIDLLKKQHNLLNSYWTDAVEQIKGFR
                     SGTFIAAPSWPYQYHILRKEGMLNYNKTIPREGVTGWADAILMPEKAKNINCAYKYLN
                     HSLNAKVQGDMAAWTGSVPVVPDACFQKNSLINSQICTENGFFHYDKITFWQSLERCD
                     SFNPCVSFERWQTDFLFLKLGMFEFLP"
     gene            complement(2701..>2954)
                     /locus_tag="PK683_05515"
     CDS             complement(2701..>2954)
                     /locus_tag="PK683_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07939.1"
                     /translation="NFTDIKEVSGFPAEGRYLFAETDRFSYLGSSLYYSNDLKTWKEN
                     KKAVSMNFNPSVYIVNDNKVLGIGSEGKIGILETSAAFP"
BASE COUNT          922 a          523 c          667 g          842 t
ORIGIN      
        1 gctgttgtaa acgattcttc tgccgtattt tacaatccgg cagggcttgg gagaaacaac
       61 tttgttcaga attatattga actgcagaaa tccaagggac cggcagtgat tatgcctttt
      121 attacaactc aggagtcttt gaaaaagaag acgccgggaa aaaagactga ggacgaaaaa
      181 aaaaacgagc ctgcatctga aaaaacagga aagggtgaat gcatagatgt gcctgtcaaa
      241 aaaggctttt tgaatgaaat tcatattatg ggcagcctga ctcagccttt actgaaagca
      301 agcggcggac agagtgagga tataaaaaga gtcaaagaca gttatgcagg gctgagcctg
      361 agcattgatt tgaataactt catcaaactg aacagaaatg cacgtttcgg tctgaatatt
      421 cttcttccag ctacaggaaa tcttctgtcc atcaatgatt taaatcccgg atctcccaga
      481 taccttcaga atgggcattc tctggaaaga cctaccatta tgggcggtgc aggcatagaa
      541 ctgtggaaag acagacttta tgctggagtc ggatttactg cgcttgtaaa agggagagga
      601 tcagctcttc tgaaaaatgt gcagctttca cctgaccaga ctgttccgaa ccagcaggtt
      661 attgttgata taaaaccgtt cgtaaatcct acattcgggc tgatggggca gtggaaaaat
      721 ttttatacgg gcatatctta taaaagagag attatgacat cagtggatcc ggtaaatgca
      781 agggctcaga cagcgatact ggcaattcaa ttagatgtaa aactggcact gtttgatcat
      841 tttaccccca gaacctggaa ctatggaatc ggatacagat ttctgcaaaa ataccttgtg
      901 acagcggatt taaacaggga attatggagt gcatttaaaa tgtcttccgt aaaaaacgaa
      961 tattcgcagc ctgttttttt tcgtgatatt tccaatccgc gagtaggttt tgaatacagt
     1021 ttcagagata atattctttt cagagcaggc tatggaaaaa gaatgaatcc tgcagggcgg
     1081 atgccgggag aagcaaacat cattgatttt gaaaggagaa ttggaagcat aggagcgaca
     1141 tacgcatttt tccctgatcc cgattcaatc atcaatactc ctgtcatact ggatttggtt
     1201 tatgagtatc attcattgaa gtctgaagtg cagaacaaat atatgcattc agcagacagt
     1261 gccaactata cggctaaagg aagaattcac catttcggtt tttcgcttac cgcatatttt
     1321 tgaattgagt attgtcgaat ctcaaaatgc aatacctgct ccaagttttc tgagttttta
     1381 aaaacaactg agcagaatgg atatatttaa aaattactta taattcagga gattttaatg
     1441 ataaatagaa agtgtataac agaattcttt tcatccgctt ccggatacga agaatacagt
     1501 caggaagaaa aaatttatga aacatttgca ggatatactg gatttttatt atcagtgatt
     1561 cttgcttttt cggtatgtac cgaaaaaaga tcaggcagac tgcagacctt ggggaaagac
     1621 gagggaaaag taatcatcgc aggctggccg ggttatgtgg aaaagggaga gaatgacagt
     1681 aaatatgact gggtttcgga atttgaaaag aataaccact gcaaggtgga gaacatcatt
     1741 ttcaaaaatg agcatgatat ttcaagagtt cttcaggatg gaaccgcaga tctggtaatt
     1801 ctttcaagtg aactggtact gaaatatgcc agagatgaaa aactgcggga gaaaaatctg
     1861 gatctgctcc cgaattgggc taatgtggac agcagagtca ggaattcgtc ctggttcaag
     1921 attctggata aaagttatgg ggttcctata ggctggtctg cgaactatct tttattcaat
     1981 aaaaactatt tttccgttga gcctgattcc tgggaactta tgtttgagga agggaaactt
     2041 tcagatgggc gcagttctgc aaaaagaatt caagcgtatg gaaatcctgt gtacattgcg
     2101 gatgctgctc tgtatttaaa atataaaaga cctgaattga agatacagaa tattatgaat
     2161 ctgaatgagg ttcagtataa ggctgtcatt gatcttctaa aaaagcagca taatcttttg
     2221 aacagttact ggactgatgc tgttgaacag attaaagggt ttcgcagcgg aacttttata
     2281 gcagctcctt cctggccgta ccaataccat attctcagaa aggaaggtat gctgaattat
     2341 aataaaacaa taccgaggga aggcgttact ggatgggctg atgcgatact gatgccggag
     2401 aaggcgaaaa atattaactg cgcatataaa tatctgaatc attccctgaa tgccaaggtt
     2461 cagggtgata tggctgcttg gacgggttct gttcctgtcg tgccggatgc ctgttttcag
     2521 aaaaacagtc tgatcaattc tcagatatgc actgaaaatg gattttttca ttatgataaa
     2581 attacattct ggcagtcact ggagcggtgt gattccttca atccctgcgt ctcctttgag
     2641 cggtggcaga cagattttct tttcctgaag ctgggaatgt ttgagttcct gccttgattc
     2701 tcaggggaat gcggcagacg tttccagaat tccgattttt ccttctgatc cgatgcctaa
     2761 taccttatta tcattcacaa tataaactga tggattaaaa ttcatactga ctgccttttt
     2821 attttctttc caggttttca ggtcattgga ataataaagc gatgagccta aataggagaa
     2881 tcggtcagtt tctgcaaaga gataccgtcc ttcagcagga aaaccggaaa cctccttaat
     2941 gtctgtaaag ttcg
//
LOCUS       DAOQGJ010000271         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20273_length_2948_cov_3.774974, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000271 DAOQGJ010000000
VERSION     DAOQGJ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20273_length_2948_cov_3.774974"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PK683_05520"
     CDS             <1..1146
                     /locus_tag="PK683_05520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH07940.1"
                     /translation="VFQEMTRINFRRLYYISIIAVPVHLLHIIFFSIRANSETQQIEK
                     WRIGILTVHSVQMTVMSALFLMFHFLKKRELPLRLLGFIEAFVFLIFLLTAVTLVSID
                     QLVTTNITPYLVLCTVVGVVLLMRPVQSFILYIASYFSFYFGIGFYQTDSVILLTNRV
                     NGISITAVGICISVLLWISNRKNLLQNRKILRQRKELTKKNAELKAVNAELEKLSIID
                     ELTLIYNRRYFNRVLLKEISRHYRSKRPISLLICDIDFFKNYNDSLGHISGDHCLRIF
                     GEVLKESLFRPFDICARYGGEEFAVILPETDEIGAQNIAERILYNLRERKILHPSSLI
                     SSYVTASIGAVTLVPETETNDFELVNKADLALYRSKTEGRNRICRFE"
     gene            1200..1460
                     /locus_tag="PK683_05525"
     CDS             1200..1460
                     /locus_tag="PK683_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07941.1"
                     /translation="MNKKLSSQDKNLKRQNSPIDKKRRTIGQGLVLGIFESKKIKIEG
                     LEISKRAFDKLVEFAQQNHPQLTIEQVVAKLIEEKFLGKSEK"
     gene            1578..2324
                     /locus_tag="PK683_05530"
     CDS             1578..2324
                     /locus_tag="PK683_05530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH07942.1"
                     /translation="MQNSKETNLEEILSKIRDLFHSESESIVSAFNFSQVDSYFIFLK
                     QYNEIGGVFSAKDSENILERHLLESLYYVFKIAERYPVSHETKIADIGTGPGLPGFLF
                     TCLKNPPKVYLVDSQKRRLKLLENYCIEKGIGQVFFLYQRAEEIQEKFDLITMRSAVP
                     YPWSAEIVYSLMDRKSIFCPFLAKLGDREKTEKILLGSLGVKIEETLHMNALSFLGER
                     NIKFLKKSVNPKHGFPRQWSVISKEIKTDG"
     gene            2317..>2948
                     /locus_tag="PK683_05535"
     CDS             2317..>2948
                     /locus_tag="PK683_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07943.1"
                     /translation="MGKIISVSNQKGGVGKTTTSINLASNLAALDKKVLIVDIDPQGN
                     AGSGLGVSINELEKTTYEVLLGEISLSEAVIPTDVKNLYIVPSNINLSGAEIDLMDKE
                     RKEYSLKNSLSKMRSQYDFIIIDCPPSLGVLTVNALTASDSVLITLQTEYFALEGLSQ
                     LMKIISLVQENLNSGLELEGVLLTMYDKRTNLANQVAADVREFFKDKVFKA"
BASE COUNT          897 a          540 c          632 g          879 t
ORIGIN      
        1 gtttttcagg agatgactag gattaatttc agacggcttt attatatttc catcattgcc
       61 gttcccgttc atcttctcca tatcattttc ttttccatcc gggccaattc ggaaacccag
      121 cagattgaaa aatggagaat cggaatactg accgttcact ctgtccagat gacagtgatg
      181 tctgccttat tcctgatgtt tcattttttg aaaaagagag agcttccgct tcgcctgctt
      241 ggattcattg aagcctttgt atttttaata tttcttctga ccgcagttac cttggtttcg
      301 attgatcagc tggtgacaac aaacatcact ccttatttag ttctatgcac tgtcgtcggt
      361 gtcgttcttt tgatgaggcc agttcagtct tttattctgt atattgcatc atacttttct
      421 ttttattttg gcataggctt ttatcagacc gattctgtaa tcctcctgac aaatcgtgtg
      481 aatggaattt cgattacagc agtcggcatt tgcatatctg tattgctttg gatttccaac
      541 aggaaaaatc ttcttcagaa tcgcaaaatt ctccggcaga gaaaagaact cacaaaaaag
      601 aatgctgagc tgaaggctgt aaatgctgaa ctggaaaaac tgagcattat tgatgaactg
      661 actctcatct ataacagaag atattttaat cgggtgcttc tgaaagaaat cagtcggcat
      721 tatcggtcaa agcgtcctat atctcttctg atctgcgaca ttgatttctt taagaactac
      781 aatgattcgc ttggtcatat atcgggagat cattgtctga gaatattcgg cgaagtactg
      841 aaagaatctc tttttcgtcc ctttgatatc tgtgcaagat atggaggaga agaatttgct
      901 gtcatacttc cggaaacgga tgaaataggt gctcagaata ttgcagagag aattctttat
      961 aatttgcggg agaggaagat tcttcatccc tcttccctga tctcgtctta tgttacagca
     1021 agcatcgggg cagtcactct cgttcccgaa actgaaacga atgattttga actcgtcaat
     1081 aaggctgatc ttgctcttta cagaagcaag acggaaggaa gaaacagaat atgcagattc
     1141 gaatgaaatc ggttgagtaa tcagtctctg ttatccgatt ataaagcaga ggaacttgta
     1201 tgaacaaaaa gctttcatct caggataaga atctcaaaag gcagaattca ccaatagata
     1261 aaaaaagacg gactatcggt cagggattgg ttctgggaat tttcgaatct aagaaaatta
     1321 agatagaagg tttggagata tcaaagaggg cttttgataa actcgtcgaa tttgcacagc
     1381 agaaccaccc gcagctgacg attgagcagg tagttgcgaa actgattgag gaaaagtttt
     1441 taggaaagtc cgaaaagtga ggaatgagac ttaaacggaa ttctgtctct ttatgtttca
     1501 cgtgaaacac ttttttatct gctgatcaaa tttaaaattt aggtttaaat taatccccgc
     1561 tgttcaagtc tgtttttatg cagaacagca aagaaacaaa cttggaagag atcctgtcga
     1621 aaatcagaga tctgtttcat tccgaatcag aatcgattgt atccgccttt aacttcagcc
     1681 aagtagacag ttactttatt tttctaaaac aatacaatga gatcggcggg gtcttttctg
     1741 ccaaagacag cgagaatatt ctggaaagac atctcctgga aagtctttac tatgttttta
     1801 aaatagcaga gagatatcct gtttcacatg aaacaaaaat tgcagatatt ggaaccggac
     1861 caggtcttcc cggctttctg tttacgtgct tgaaaaatcc tcctaaagtt tatctggtgg
     1921 actcacagaa aagacggctt aaacttctgg aaaattactg cattgaaaaa ggaattggtc
     1981 aggtgttctt tctgtatcag cgggcagaag agattcagga aaagtttgat ctaattacaa
     2041 tgagatcggc ggttccttat ccatggtcgg cggagatagt ctattcactg atggacagga
     2101 agtcaatttt ctgtcccttc cttgcaaagc tgggagatag ggagaagacg gaaaagattc
     2161 tcctcggctc cttgggagtc aaaatagaag aaactctgca tatgaatgca ctttcttttt
     2221 tgggagagag aaatattaaa ttcttgaaaa agtcagtgaa ccccaagcat ggttttccaa
     2281 gacaatggtc agtaatcagc aaggagataa agacagatgg gtaagattat atcggtaagc
     2341 aaccaaaaag gcggtgtagg aaaaacaact acctccataa atttggcatc caatcttgcc
     2401 gccttggata agaaagttct gattgtagac atagatcctc agggcaatgc aggatcaggt
     2461 ttaggagtca gcatcaatga acttgaaaag acaacatacg aagttctgct tggtgagatc
     2521 tctttgagtg aggccgtgat tccgaccgat gtaaaaaatc tgtacattgt tccttccaat
     2581 ataaatttga gcggtgccga aatcgatttg atggataagg aaagaaaaga atattcgctc
     2641 aaaaattcac taagcaagat gagaagccag tatgatttta tcataataga ctgtcctcct
     2701 tccttgggcg tactgactgt aaatgcgctg acagcatccg attctgttct gatcacactt
     2761 cagacagaat actttgcttt ggaaggttta agtcagctga tgaagattat ttcgcttgtt
     2821 caggaaaatc tgaattccgg cctggaactc gaaggtgtgc ttctcactat gtatgataaa
     2881 aggacaaact tggcaaatca ggttgcggct gatgttagag agttttttaa ggacaaggta
     2941 ttcaaggc
//
LOCUS       DAOQGJ010000272         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20275_length_2948_cov_3.330798, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000272 DAOQGJ010000000
VERSION     DAOQGJ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20275_length_2948_cov_3.330798"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PK683_05540"
     CDS             complement(<1..246)
                     /locus_tag="PK683_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07944.1"
                     /translation="MESIGNLLLFIQNLDPVHALLAVLSVFPWGFFFYMFQPESSRKT
                     SVSLTALLAGILSTQIIMGLHPVFWPEVDFKPRKTSLL"
     gene            543..2531
                     /locus_tag="PK683_05545"
     CDS             543..2531
                     /locus_tag="PK683_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNH07945.1"
                     /translation="MASDANRLNLKTKAYNKTESLLDAGRKNLNEIPREVFEQKKLKH
                     LILCYNQIRNLPDTFDRLPHLKSLDLCGNQLQSLPESIRNLKKLEELNLSGNLIAEFP
                     KLILELPNLKILKLNDLNLASVPKELGKLKHLSRLEIFSLQKFDMKSLITAFAHSEKE
                     LFLTQGEEINTKGRNAFVIVLPKTIDKIPKEIYRLKNLIHLSVCDSGLKTVSKEIGAL
                     RKMEGLFLSGNQIVSLPAEIGRLKNLKCTDLSFNKFSAFPKILNGLRKMEEIIMNGNS
                     IETVPAEIGGFSELKTLSFDGNRIRNVPPELGKLLKLEELSLSFNQIAEVPEELSALK
                     NLKILNLEGNKLKNIPMKFKSMKKIFILDLAENDFSEFPEVISELPQLVFLDLEYNRI
                     GSLPDKIGRLKKLKVLKLGYNRLSSIPAGISGMTELETLILSKNRIRAVPPELSGMRK
                     LLYLNLEYNRIEEIPKEICSMNHLLELYLEGNLLKTVPKEIENMTGLYALAIFKKNPL
                     EHLPKEFYNLNIFHEFNWDSTGQEFEPSSSPWDRKIIDDAYKRIKECGRKKNSSLVLT
                     DRRLFAVPSEICHMTHLKELDLSGNFLRFLCSDLENLENLEVLRLNRLNYLGRISCDF
                     TKFRKLKTLELIGTSVSGKEKERLKNWSAKSGCSVKFK"
     gene            2678..>2948
                     /locus_tag="PK683_05550"
     CDS             2678..>2948
                     /locus_tag="PK683_05550"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNH07946.1"
                     /translation="MKKSGSASLDAGNKDLSEIPDEILNLKQLTGLNLCSNQLKALPE
                     NIDSLSLLRSLDLSNNQLNSLPESFGRLKNLEELNLNDNQFTEIPE"
BASE COUNT          998 a          594 c          636 g          720 t
ORIGIN      
        1 cagaagactc gtttttctgg gtttaaaatc cacctcaggc cagaatacag gatgaagccc
       61 catgataatc tgagtcgaaa gaattcctgc cagaagtgca gtcagtgaaa cggaagtctt
      121 tctgctgctc tccggctgaa acatatagaa aaaaaatccc cagggaaata cagacagaac
      181 tgcaagaagc gcatgaactg gatccagatt ttgaatgaag agaagaagat tgccgatcga
      241 ttccactgga attcagtttt tttaattgcg gactttggtc aagcatacaa ataagcataa
      301 ttgtattgca aaagaagctt tctctgaaat attcagctca atcggacgta ggaagtctga
      361 tattggaaat gagcagactt agaacagaca gtgattcaat caagcccctg tcatctgcct
      421 gaaaaagtct gtaaaaaggg agctgccgca aacttggaag ccgccgcaaa cttggaagcc
      481 gctgcagaca ctgaagcttc ggcagttaat aaaaaaatat ataatgaagg atactgactg
      541 agatggcctc agatgcgaac agactgaatc tgaagacaaa agcttataat aagacggaat
      601 ctctgctgga tgcaggcagg aagaatctga atgaaatccc cagagaagtc tttgagcaga
      661 aaaaactgaa acatctgatc ctgtgctata atcagatcag aaatcttccg gatacttttg
      721 acagactccc tcatttgaaa tcactcgacc tctgcggcaa tcagcttcaa agtcttcctg
      781 aaagtatccg taatctgaaa aaactggaag aactcaacct gagcggaaac ctgatagctg
      841 aatttccaaa actgattctg gaactcccca atctgaaaat tctgaagctg aatgatctga
      901 acctggcctc tgttccgaag gaactgggaa aactgaaaca tctttcccga ctggagattt
      961 tcagtctgca gaaatttgat atgaaaagcc tcattactgc atttgcccat tctgaaaagg
     1021 aactctttct gactcaggga gaagagatca atacaaaagg caggaatgcg ttcgtgattg
     1081 ttcttcccaa gacaatcgat aagatcccga aggaaattta taggctgaag aatctgattc
     1141 atctgagtgt ctgcgattcc ggtctgaaaa ccgtctcgaa agaaatcggg gcactgagaa
     1201 agatggaagg actttttctt tcaggaaatc aaatcgtctc cctccctgct gaaatcggga
     1261 gactgaaaaa tctgaaatgc acagaccttt ccttcaataa attcagtgca tttccaaaga
     1321 ttctgaacgg cctgagaaaa atggaagaga tcataatgaa tggaaactcc attgaaacag
     1381 ttcctgcaga gatcggcggt ttttctgaac tgaaaaccct aagttttgac ggaaacagaa
     1441 tcagaaatgt cccgccggaa ctcgggaaac ttctcaagct ggaggaactg tccttatcct
     1501 tcaatcagat tgcagaggtt cctgaagaac tctctgcgct gaagaatctg aaaatactga
     1561 atctggaagg aaataaatta aaaaatatcc cgatgaaatt caaaagcatg aaaaagattt
     1621 tcatcctgga tcttgctgaa aatgatttct cggaatttcc tgaagtgatt tcagaactgc
     1681 cgcagcttgt gtttctggat ctggaataca acagaatcgg atcacttccg gataaaatcg
     1741 gcagactgaa aaaactgaaa gtactcaaac tgggatacaa ccgattatcc tcaattccgg
     1801 caggcatttc gggaatgact gaactggaaa ctctcattct cagcaaaaac agaatacgtg
     1861 cagttccgcc ggaactttcg gggatgcgga aacttttata cctcaatctt gaatacaaca
     1921 ggatagaaga aattcctaaa gaaatctgca gtatgaatca tcttctggag ctttatctgg
     1981 aaggaaatct tctgaaaaca gttccgaagg aaattgaaaa tatgactgga ctttatgctc
     2041 ttgccatatt caaaaagaac cctctggaac atcttcctaa ggaattttat aatctgaaca
     2101 tatttcatga attcaactgg gacagcacag gtcaggaatt tgaaccgtca agcagtccct
     2161 gggacagaaa gataatagat gatgcttata aacgaatcaa agagtgcgga agaaaaaaaa
     2221 attcatccct tgttctgacg gacagaagac tttttgctgt tccctcggaa atctgtcata
     2281 tgactcatct gaaggaactc gatctctcag gaaactttct cagattcctc tgcagtgatc
     2341 tggaaaatct ggaaaatctg gaagttctgc ggctgaatcg gctgaattat ttgggacgca
     2401 taagctgtga ttttacgaaa ttcagaaaat tgaaaactct tgaactgatc ggaacctcag
     2461 tatccggaaa agaaaaagaa cggctgaaga actggtctgc aaaaagcggc tgctctgtaa
     2521 aatttaaata ggactgtcta tgaaaaatga tctgcctgga gacacagact gtaatacaga
     2581 catcaccggt gacggtgcag ggaagtaaaa aaatatataa tgaggaatac tgacaatgag
     2641 cgtgaatgga aagaaaacag aatataaggt aaagacggtg aaaaagtccg gcagtgcttc
     2701 gctcgatgcg ggtaacaaag acctgagtga aattccggat gaaatactga atctgaagca
     2761 gctgacaggc ctgaatctct gctcaaatca gttgaaggca ctgccagaga acattgacag
     2821 tctttctctt ctgaggtcgc tcgatctcag caataatcag ctgaacagtc ttccggaaag
     2881 tttcggccgg ctgaaaaacc tcgaagaact caatctgaac gacaatcagt tcactgaaat
     2941 tcctgagc
//
LOCUS       DAOQGJ010000273         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20377_length_2939_cov_3.604716, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000273 DAOQGJ010000000
VERSION     DAOQGJ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20377_length_2939_cov_3.604716"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PK683_05555"
     CDS             <1..1098
                     /locus_tag="PK683_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNH07947.1"
                     /translation="ELNYSTQAVRNNEFNINLAFIDAKLSTEKVRGRLAFQYGTSVNA
                     NYTPELNQRYSNQLSVRNIQEAYAGIKIGKKTWVDAGIYFGNIGFESWISGNNWLYTR
                     ALVLDYVPYYSTGVRISHEVTDKLNVQFHLMNGWSNITDNNRNKAVGMQVSYKMRKDL
                     LLTYNNFIGNEIATSSRTDSAGQTYVNSKGNERQTRYYHNFIAHWDMNEKFSLAGSFD
                     VGFVRNADSLIYSPSVPQDINKDYPYLRKAAGGSFKQWYNGTVWLTYKFIEDWRISGR
                     LERYIDAEQANVPIVKQSEVFTKRPGKQPNGFQVSAVSVCLDYIPSEYGLMRVEAKYM
                     KSADPIFEYRDSGDLSRHEKKLIFNVTLKHH"
     gene            complement(1073..2488)
                     /locus_tag="PK683_05560"
     CDS             complement(1073..2488)
                     /locus_tag="PK683_05560"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH07948.1"
                     /translation="MNQDDYSREISAQRPSNIARLLAGFVFLTVSVIYRLNAIFFISV
                     FFITASAVWLYFVERKYSIIKKHENLWFFIASIDITTVSLFVYFTGAAYSPVIMGYIF
                     MVTLSSTDLKRIRGRFSAAFSIFSFLVILILVTAGIIEYVNILSSSGEKPGIFASVIA
                     FLLLLIACFSVNDMIYNIYYQYNEKNTALSSALDSVKKLKDQQDGDYFLTSLLLEPFY
                     SEQKETGLISMEILMRQYKRFIFKGKEYDLGGDACLSDVIFVKGEKYFIFLNGDAMGK
                     SMQGAGGAVVLCSVFRSITEHLRTSVNTAEFRPELWLLQSFGDLHKVFESFMGLMLAS
                     CIFGILEESSGELLFMNAEHPFLILYRDEKARFIESESSMNRKLGTLEMNSDPIVQRF
                     HLVHGDILFLGSDGKDDLGLADPSGMRVVDSEETRILSVIESAKGDIQKTAELLFTFG
                     AQVDDLSIMKIKYFNDASKSH"
     gene            complement(2746..>2939)
                     /locus_tag="PK683_05565"
     CDS             complement(2746..>2939)
                     /locus_tag="PK683_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNH07949.1"
                     /translation="STLIGERPKVSPHRQKIRESMAEILGLPVEKVSLKATTVEKMGA
                     IGRKEGIAVISTVLLGSVH"
BASE COUNT          871 a          649 c          636 g          783 t
ORIGIN      
        1 gagctgaact acagcaccca ggcagtcaga aacaatgaat tcaatatcaa tctggctttc
       61 atcgatgcaa aactttccac tgaaaaagtg agaggccgtc ttgcatttca gtacgggacc
      121 tcggtcaatg caaactatac tcctgaactg aaccagagat attccaatca gctttccgtc
      181 aggaacatcc aggaagcata cgctggaatc aagatcggga aaaagacctg ggtggatgcg
      241 gggatttatt ttggaaacat aggatttgaa tcatggattt ccggaaacaa ctggctctat
      301 actagagccc ttgttttgga ctatgttccc tactattcca ctggagtccg aatatcacat
      361 gaggttactg ataaactgaa tgttcagttt catctgatga acggatggtc aaacatcaca
      421 gacaacaaca ggaacaaggc ggtcggaatg caggtctcgt ataaaatgag aaaggatctt
      481 cttctgacct acaacaattt tataggaaat gaaatcgcca catcctccag gacagacagt
      541 gcagggcaga cctatgtgaa ctcaaaagga aatgaaagac agaccaggta ctatcacaat
      601 tttatcgctc actgggatat gaatgaaaaa ttttcactcg caggttcatt tgatgtcggt
      661 tttgtaagga atgcagattc gcttatatat agcccttctg ttccccagga catcaacaag
      721 gattatcctt atctgagaaa agcagcaggc ggttccttca aacagtggta caacggcaca
      781 gtatggctga cgtataaatt cattgaagac tggaggattt ccggcagact cgaaagatac
      841 atagacgcag agcaggcaaa tgtgcccatt gtgaaacaga gcgaggtatt cacaaaacgt
      901 cctggaaaac agccgaacgg ttttcaggtc agtgcagttt ctgtatgtct ggactatatt
      961 ccttcagagt acggactgat gagagtggag gcaaagtaca tgaaatccgc agatccgatc
     1021 tttgaataca gagacagcgg ggatctgtcc cgtcatgaaa aaaaactgat ctttaatgtg
     1081 actttgaagc atcattaaaa tattttattt tcatgatgct gagatcgtct acctgggctc
     1141 cgaaagtaaa aagaagttct gcagtcttct gaatatctcc ctttgccgat tcgattacag
     1201 aaagaatcct ggtctcctct gaatccacga ccctcatgcc ggaaggatcg gccagtccaa
     1261 gatcatcctt cccgtcggag cccagaaaca gaatgtctcc gtgcacaaga tgaaatctct
     1321 gcacaatcgg atcactgttc atttccaagg ttcccagttt cctgttcatg ctgctttccg
     1381 attcaatgaa tctggccttt tcatccctgt aaagaatcag gaaaggatgt tctgcattca
     1441 taaaaaggag ttcacctgaa ctctcctcaa ggattccgaa aatgcaggat gccagcatca
     1501 gtcccatgaa ggattcgaac accttatgaa gatctccgaa agactgaagg agccagagtt
     1561 ccggccggaa ttccgctgta ttcaccgatg ttctgagatg ctctgtgatg gaacggaata
     1621 ctgagcagag aacaactgca ccgcctgctc cctgcatgga ttttcccatc gcgtctccgt
     1681 tcagaaaaat gaagtatttt tctcccttta cgaaaattac atctgaaaga caggcatctc
     1741 ctcccaggtc atattccttt cccttgaaga tgaatctttt gtactgtcgc atcagaattt
     1801 ccatactgat gagtcccgtc tccttctgtt cgctgtaaaa aggctcaagc agaagagatg
     1861 taagaaaata gtctccgtcc tgctggtcct tcagtttttt cactgaatcg agggcggagc
     1921 tgagagcagt gtttttttca ttgtactgat agtatatatt gtatatcata tcgtttacag
     1981 agaaacaggc aatgagaagg agcagaaaag ctatcacgga agcaaagatt cccggcttct
     2041 cgccggaaga agaaagtatg ttcacatact ctataatgcc agctgtcaca aggataagta
     2101 tgacaagaaa tgagaaaatg ctgaatgccg ccgaaaatct cccgcgaatg cgcttgagat
     2161 cagtggaact cagagtcacc atgaaaatgt accccataat gacaggcgag taagcggccc
     2221 cggtgaaata tacaaacaga gatactgtag tgatgtctat tgatgcaatg aaaaaccaga
     2281 ggttttcgtg ttttttaatt atactgtatt ttctctccac aaaatagagc catactgcgg
     2341 atgcggtgat gaaaaacacc gaaatgaaga atattgcatt gagtctgtaa atgactgaaa
     2401 ctgtaaggaa tacaaatccg gcaagaagtc tggcaatatt ggacgggcgc tgcgcgctga
     2461 tttccctgct gtaatcatcc tgattcattc tcttcggcct gtttctgtaa agatttttat
     2521 caaccttttt tcagtcaact gcaccgcaat cttcagatgt ctcattttga atttactgct
     2581 gattatctgc ttaaacggga aaactgtctc ctgagaaatc atcgctgatt cggcttcttt
     2641 gtcaaattat ttacaaagag acgaagctgg gcgaaattca gaagattccc tttacagaaa
     2701 agagaatact gccgccggga tcactgtttt tcagtcaatg ctgcttcagt gaacagaccc
     2761 gagaaggaca gtagagatca ctgcgattcc ttcctttctt ccaatggctc ccattttttc
     2821 caccgtagtc gccttaaggg aaaccttttc gacaggaaga ccgagaatct ctgccattga
     2881 ttctcggatt ttctgcctgt gaggagacac cttcggtctt tcaccgatca gtgtagaat
//
LOCUS       DAOQGJ010000274         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20385_length_2938_cov_3.955255, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000274 DAOQGJ010000000
VERSION     DAOQGJ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20385_length_2938_cov_3.955255"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..462)
                     /locus_tag="PK683_05570"
     CDS             complement(76..462)
                     /locus_tag="PK683_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07950.1"
                     /translation="MNRIFRTAVLPITAASVWISISEFIRNEIIFKSYWINHYEKLGM
                     KFPSEPLNGAVWGLWSVMFAAAVFIISGKLSFLQTALFSWYIGFVLMWTVIGNLGVLP
                     DGLLYAAVPLSLLESFVAAFIIKKLR"
     gene            1056..1430
                     /locus_tag="PK683_05575"
     CDS             1056..1430
                     /locus_tag="PK683_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07951.1"
                     /translation="MKHLFIAFSGLLFIFFCAKYPAVKEKEIQPSLHIRSLEKVKELA
                     AGACGSCHTSGLETSKPAALKVFDLNDSDWMKNMNEIQLSRTFIQRLGFTVQKKDRET
                     VTDCLGEELYLRKNKNVKKAAF"
     gene            1430..2044
                     /locus_tag="PK683_05580"
     CDS             1430..2044
                     /locus_tag="PK683_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH07952.1"
                     /translation="MIKYNIAIADDHSVAQLGIETALSKSPIFQIIKKFSTGAEISFG
                     LKESDIHILILDMDLPGQNGMDFLKDNRSVFPALKIVLFTAHEGEGFFREAVRLGADG
                     YILKSDNVMNLHDSLMHIVDGKFCCSPKLLKFLNDPKSGISLSERESEVLKLAVNGHT
                     SREIGERIGISRRTAEYYISRLKEKFDAENLLQLAEAAREKYFK"
     gene            2051..>2938
                     /locus_tag="PK683_05585"
     CDS             2051..>2938
                     /locus_tag="PK683_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNH07953.1"
                     /translation="MKSIYIFLIIIFNFSLSGYEEFSVKDSEISAPLGKYSYFLEDGS
                     KSFNLDQVRKLPEEIWKKNNADTLNLSFSDKAFWICIKIHNGGKKRGTYFIELNSSRI
                     DYIEFYSQHSNYEKERSGILVKFSERKVRHKNPVFTVSLLPESHNLLYFRIESGTVMQ
                     FNPVIHTEGRLTEKTSKEELLNGIMFGIIILSCMYGLLLYLIINDLANLYFAIYSAVI
                     FCFFLVLTGTGFHYIWPNFPIVNHTNVFFGSLGNIFGLMFQYYIMDFRKNLPKLEILS
                     KTLITLNILTAVMFSFIPFS"
BASE COUNT          934 a          602 c          569 g          833 t
ORIGIN      
        1 tatgtccgga attgatccag agctcctgcc cgccgtaggc atcgattgct ttttttgcag
       61 tttcagtcag cataatcatc tcaatttttt tataataaat gcagctacaa aagattcaag
      121 aagactcaga ggaacagcgg catacagcag cccgtcagga agaacaccaa gatttccgat
      181 cactgtccac attaaaacga atccgatata ccaggaaaaa agtgcagtct gaagaaaaga
      241 caatttcccg gaaatgataa acaccgcagc cgcaaacatt actgaccaga gtccccatac
      301 tgctccgttt aaaggctcag acgggaattt cattccaagt ttttcataat gatttatcca
      361 atacgattta aatattattt catttctaat aaactcagaa atgctgatcc atactgaagc
      421 tgcagtaatc ggaagcactg cagtcctgaa tattctgttc ataaattcct cacaggtcaa
      481 atgttgtcga tgtctatatt gcaggatcaa gtccctgagt caatgcaaat gtatgcatcc
      541 atgacccctg ttctttaaat aaaaagcatt tctgtcctct gagaaattca taagcataca
      601 gaaaataagt cactcgcctt actcccttgg cttcaggcag gactttttag aagagagtct
      661 gtcggcggtt tcacagccct tgaagatgct aatggttctg cccccaccaa gccttatcaa
      721 gacggctgag gttttggaag ccgactcgac agctctgaaa ctgctgtttt actgattcat
      781 cgcaaactat ttaactgcag atttccctaa tcttttctga aaattacagc gtctagacag
      841 catatcatat ttgtgcaaga taagtaaggc ataaaataca cacagcaccg cttcacattc
      901 cggggccggg gctgaagagt ctcctgtttc aaaataatac ggaagctcat ataaaacttg
      961 acttttcttc ggactctgaa taatgaagtc agaagaaact gccttttttt ggactctctc
     1021 aaaaggatac actgataatt cagtataata gcattatgaa acatcttttc attgcatttt
     1081 caggactcct gttcattttt ttctgtgcaa aataccctgc cgtaaaggaa aaagaaattc
     1141 agccatctct gcatattcgg agtctggaaa aagttaaaga actcgctgcg ggtgcctgcg
     1201 ggagctgtca tacatcagga ctggaaacat ccaagcctgc cgcactgaaa gtctttgatc
     1261 tgaatgattc agactggatg aaaaatatga atgaaattca gctgagcagg acattcattc
     1321 aaagactggg ctttactgtt caaaaaaaag acagagaaac agtaactgac tgtttaggag
     1381 aagaactcta tctcagaaaa aacaaaaacg tcaaaaaggc ggcgttttaa tgataaaata
     1441 taatattgca atagcggatg atcactcggt agcacagttg ggaattgaaa cagctctgtc
     1501 caaatctcca attttccaaa tcattaaaaa gttttcaaca ggagcggaaa tcagtttcgg
     1561 actgaaagaa tcggacattc atattctcat actggatatg gatcttcccg gacagaacgg
     1621 aatggatttt ctgaaagaca accgctcagt tttcccggcc ttgaagatag tgcttttcac
     1681 tgcacacgaa ggcgagggct tcttccgtga ggcagtccga ctgggtgcag acggttatat
     1741 actgaaatca gataatgtaa tgaatctcca tgacagttta atgcatatag tggatggaaa
     1801 attctgctgc tctccaaaac ttttgaagtt cctgaatgac cctaaatccg gaatttcgct
     1861 ttctgaacgg gaatcagaag tactgaaact cgcggtaaac ggccacacat cccgagaaat
     1921 aggcgaacgg ataggaattt ccagaagaac cgctgagtat tatatttcga ggctgaagga
     1981 aaaattcgat gcggaaaatc ttcttcagct cgctgaagcc gccagagaga aatattttaa
     2041 atagtcatat atgaagtcta tatatatttt tttaattatt atttttaatt tcagtctttc
     2101 cgggtatgaa gaattcagtg tcaaggattc tgaaatctcc gctccgctcg gaaaatattc
     2161 atatttcctt gaagacggca gtaaaagctt caatttggat caggtaagaa agcttcccga
     2221 ggaaatctgg aaaaaaaaca atgcagacac tctcaacctg agtttcagcg acaaagcatt
     2281 ctggatctgc ataaaaattc ataacggagg aaaaaagaga ggaacttatt tcattgaact
     2341 gaattcttcc agaatagact atatagagtt ttacagtcag cattccaatt atgaaaagga
     2401 aagatcaggc atactcgtga aattttccga acggaaggtc aggcataaga atcctgtttt
     2461 tacggtcagt cttctccctg aatctcacaa tctgctttat ttcagaatag agagcggaac
     2521 ggttatgcag ttcaaccctg taattcatac cgaagggaga cttacggaaa aaacctcaaa
     2581 ggaagagctt ctgaacggga ttatgttcgg aatcattata ctttcatgca tgtacggtct
     2641 cctgctgtat ctgattatca acgatcttgc aaatctgtac tttgccatat acagtgcagt
     2701 catattctgt ttttttcttg ttctgacagg aaccggattt cactatatct ggccgaactt
     2761 tccgattgta aatcacacaa atgtgttctt cggctcactg ggaaatatct ttggactgat
     2821 gtttcagtat tatataatgg atttccgaaa aaatcttcca aaattggaaa ttctttcaaa
     2881 aacactgatc accttgaata tactgactgc agtaatgttt tccttcattc cgttcagt
//
LOCUS       DAOQGJ010000275         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20409_length_2937_cov_2.517349, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000275 DAOQGJ010000000
VERSION     DAOQGJ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20409_length_2937_cov_2.517349"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..477)
                     /locus_tag="PK683_05590"
     CDS             complement(64..477)
                     /locus_tag="PK683_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNH07954.1"
                     /translation="MVSHILESVSGLANFGLYFFSAVGLLIVFSYVYGMVTPYPEIQL
                     IKEGKMAPAISYGGAVFGFICPLVSAIAHSVSFIDMLIWAFIALVIQILVFLFLKTVF
                     SSLVEDVSQDKISSAIFLSVFSVAVGLLNAASMSY"
     gene            complement(478..1602)
                     /locus_tag="PK683_05595"
     CDS             complement(478..1602)
                     /locus_tag="PK683_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HNH07955.1"
                     /translation="MERIRTEKRPDWKEKAESAGFLYHTFDGETYWDESAYYSFTSEE
                     IDHIEEVTEEVHDMCIRAAEHIIRKNLFSKLCIPEKYVPYIIKSWENNDPSLYGRFDF
                     SYNGSDAPKMLEYNADTPTSVLEASVVQWFWLKDKFPEKDQFNSIHEKLQEFWKESGF
                     RGTVHFACADESAEDLGNLEYLRDTAIQSGFETKQIFIEEVGWNSKLKRFVDGDDIPI
                     YNIFKLYPWEWMFEEEFGEYLPVCGWNIIEPPWKSLISTKAILPVLWDLYEGHQNLLP
                     CYFDNKFTDNFVKKPLYSREGNSISIFKDGKVTENPGSYGKEGFVYQKYCPLPEFEGN
                     FPALGSWVVNGKPAGIGIREDSTEITSNNGRFLPHLFEEE"
     gene            complement(1607..1921)
                     /locus_tag="PK683_05600"
     CDS             complement(1607..1921)
                     /locus_tag="PK683_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07956.1"
                     /translation="MSESVLKKKSTLNISLVLIGTLAVGCGEDGQRQIYKTKADCAAE
                     WSDKDCEEIPSTSSHYHTGRYMGPFHSYSYSSGRSTRSVGSSHVTRGGFGRSSSFHSS
                     GG"
     gene            2155..>2937
                     /locus_tag="PK683_05605"
     CDS             2155..>2937
                     /locus_tag="PK683_05605"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH07957.1"
                     /translation="MNSVFSILISVSFLFLRCSSAERLSENKALYESINPEKPFSDSR
                     FEEINGIKIHYRYGQTKENAYSAKILMIHGLAGSTYSFRHTAEFVNKNRMLAVSVDLP
                     AFGYSSRKLGLNHSSYDRALLISELMKRIDASLPKKTALLKWNLLGHSMGGRTVLSMA
                     LEKDISERIQSLTLIAPAAYFKGSKIGKFLLRISLIRSAAETVMAENYLNRKSFDKLL
                     ESAYARKPEPEETAGYLTPLLSPNSEKAFFDVLINSDESSAAS"
BASE COUNT          861 a          687 c          563 g          826 t
ORIGIN      
        1 cttccacttt tcaaacatcc gaaagtctgc cattgtattt tcagcctgac tgcagatctt
       61 tcttcagtaa ctcatactcg ctgcattcag aagcccgact gcaacagaga atacggaaag
      121 aaaaattgcc gatgaaatct tatcctgtga tacatcctcc acaagacttg aaaatacagt
      181 tttcagaaaa agaaaaacaa gtatctgaat tacaagtgca atgaaagccc agatcagcat
      241 atcaatgaaa cttacgctgt gagcaattgc actgaccaaa ggacagataa agccgaaaac
      301 cgctcctccg taactgatcg ccggggccat tttgccttcc ttgataagct ggatctcggg
      361 atacggtgta accataccgt atacataaga aaatacaatc agcagaccga ctgcgctgaa
      421 gaaatacagg ccgaaatttg ccagacctga taccgattct aaaatatgtg aaaccattta
      481 ctcctcctcg aataaatgcg gcagaaacct tccgttattg gatgtgattt ctgtactgtc
      541 ttctctgatg ccgattccgg cgggtttccc gttgacaacc caggaaccga gtgcaggaaa
      601 attgccctca aattcgggaa gcgggcagta tttctgatat acaaaaccct cttttccgta
      661 acttccggga ttttccgtga cctttccgtc tttgaaaatg cttatgctgt ttccttctct
      721 ggaatacaga ggtttcttta caaaattatc tgtaaactta ttgtcaaaat aacagggcag
      781 aagattctga tgtccttcat aaagatccca tagaacagga agaatcgcct ttgtgctgat
      841 caggcttttc cagggaggct caataatgtt ccatccgcag acaggcaggt attctccgaa
      901 ctcttcctca aacatccatt cccaaggata cagtttaaat atattgtata taggaatatc
      961 atcaccgtca acaaatctct tcagttttga attccagccg acttcttcaa tgaaaatctg
     1021 ctttgtctca aaaccggact gaattgcagt atctctcaga tactccagat tgccaagatc
     1081 ctctgcagat tcatctgcac atgcaaaatg aacagtcccg cgaaaaccgg attctttcca
     1141 gaattcctgc agcttttcat gaattgaatt gaactgatcc ttttcgggaa acttgtcctt
     1201 cagccagaac cactgcacca cgcttgcttc gagcacagat gtaggcgtat ctgcattgta
     1261 ttcgagcatt ttgggtgcat cggaaccgtt ataggaaaaa tcgaatcttc cgtacaggga
     1321 cggatcatta ttttcccatg attttatgat ataaggaaca tatttttcag gtatgcacag
     1381 ttttgagaaa agattcttcc gtatgatatg ttccgcagca cggatgcaca tatcatgcac
     1441 ctcttcagtc acttcctcga tatgatcaat ttcctcggaa gtaaaactgt agtatgcaga
     1501 ctcatcccaa tatgtctcac cgtcaaaagt atgataaagg aatcctgctg attccgcctt
     1561 ttccttccag tcgggccttt tttccgttct gatacgctcc aatctttcag ccgcctgaac
     1621 tgtggaacga agaagatctg ccgaatccgc cccttgtcac atgacttgat ccgactgaac
     1681 gggtagatct gccggaactg taagaatacg aatgaaaagg tcccatatat cttcctgtat
     1741 gataatgaga agatgtgctg gggatttcct cgcagtcctt gtcggaccat tcagcagcac
     1801 agtctgcctt ggtcttgtat atctgcctct gaccgtcctc accgcatccg acagccaagg
     1861 ttccgatcag aacaagagat atattcaatg tgctcttctt cttcaaaact gattctgaca
     1921 ttttaaactc ctgatttgca tgattgtaat ttcagcaaag tctgcgagta tccgttcggg
     1981 acagcccgcg aagctgtctg ctttaagaca gacactcatt cagtaacatg caatttcact
     2041 gaaataacgt caagcaatcc tttgatgttt ttccctaagc gaatctgcat cagtgaaaaa
     2101 ttgagtattt tatttgcata atatcaggag cagaattatc agtaaaataa taaaatgaac
     2161 tctgtatttt ccattctgat atccgtctct tttctttttt tacggtgcag ttcagcagaa
     2221 cgtctctccg agaacaaggc tctgtatgaa agcatcaatc ctgaaaaacc cttttccgac
     2281 agccgttttg aggaaatcaa tgggataaaa attcattaca gatacggaca gacgaaggaa
     2341 aacgcttaca gtgcaaaaat actgatgatc cacgggctgg caggttccac ttattccttc
     2401 agacatactg cagaattcgt gaataaaaac aggatgcttg ctgtcagcgt cgatcttcct
     2461 gcattcggat acagctcaag aaaactcgga ctgaatcatt cctcatacga cagagccctg
     2521 ctgatttctg aattaatgaa aagaatagat gcttcccttc cgaaaaagac tgctcttctg
     2581 aaatggaatc ttctaggaca ttcaatggga ggcaggacag tattgtccat ggctctcgaa
     2641 aaggacatat cggagagaat tcaaagtctt acactgattg ctcctgcagc atatttcaaa
     2701 ggatcaaaaa ttgggaaatt ccttctccgt atttccctta tacggtcggc tgccgaaaca
     2761 gttatggcag aaaattatct gaacagaaaa tcatttgata agcttctgga atccgcatat
     2821 gcacgaaaac ctgaaccgga agagacagcc ggatatctca ctcctctgct cagtccgaat
     2881 tcagaaaaag ccttctttga tgtgctgatc aattctgatg aatcttctgc agcatcg
//
LOCUS       DAOQGJ010000276         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20435_length_2935_cov_2.893750, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000276 DAOQGJ010000000
VERSION     DAOQGJ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20435_length_2935_cov_2.893750"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PK683_05610"
     CDS             complement(<1..644)
                     /locus_tag="PK683_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNH07958.1"
                     /translation="MSIEILDVTLRDGEQTRGVSFSTAEKLNVAKYLLLQLKADRVEV
                     ASARVSRGEQSTVKTIINWAEKENLADKIEILGFVDKTRTVEWMKESGAGVLNLLTKG
                     SKKHLTLQLNKTEKEHLSDIEETVNFAVKNKIRVNVYLEDWSNGFRNSPDYVEEMVTG
                     LIKFPIDKIFLPDTLGVFSPDETFEAISFMVNRFPGIHFEFHGHNDYDLSVANCL"
     gene            complement(646..921)
                     /locus_tag="PK683_05615"
     CDS             complement(646..921)
                     /locus_tag="PK683_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNH07959.1"
                     /translation="MREIYIRKLRYEDAKQKLEREIHNAFMEGETLVEVVHGIGEGKL
                     KQLTLDVAVTLDFIRVCEYGDFIYTNPGSTKLEILSPGKQNLKRYKN"
     gene            complement(1054..1767)
                     /locus_tag="PK683_05620"
     CDS             complement(1054..1767)
                     /locus_tag="PK683_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH07960.1"
                     /translation="MNHSFLKKQILAVMLVSASFCSKPPENKQSVSAIFIKGKAAVIS
                     SAGEHPLKKGDVLNSGDVISTGEKSAVIIETGSISGQLEIQSNSKLKVESTLKGYEFF
                     LEAGNVWISVNKLSKDQEFKLKTPTTVAGVRGTKFYTFNQGEMTGTCHCEGQIEYSNN
                     MSHKSEVNSGDYLVIHKGDISITVKEEDFKKAGIKFYGHNHSEMDDSSLGPKNGMTEE
                     EHSKVRQLIAQKFQDLEKK"
     gene            complement(1911..2786)
                     /locus_tag="PK683_05625"
     CDS             complement(1911..2786)
                     /locus_tag="PK683_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNH07961.1"
                     /translation="MSKTSVLGRGLGNLIQGGGSKNTGSSDDGSNSNLKEIKIADIRL
                     NPNQPRKNFSQETILELAETIKAHGLIQPIVVKKTAEGFELVAGERRLRACREAGFVK
                     IPAIVKNFTERESIEVALLENIQREDLNPMEEASAYQKIIDKYSIKVTELAERLGKNR
                     STVANLIRLLQLPAEVQEMVSKEKLSEGQARPLLSLYDKKKMIEVAEEVAKKGMTVRE
                     VEELVAKLTTPAKSKKAGAKTDPNILELESKIRNKVSGKVKIQHNEKNGKGRIVVSYS
                     DLEELEKILSRIGIR"
BASE COUNT          740 a          681 c          524 g          990 t
ORIGIN      
        1 agacagttcg caacggaaag gtcataatca ttatgaccat gaaattcaaa atgaatccca
       61 ggaaatctgt ttaccataaa actgattgcc tcaaaagttt catccggcga aaaaactccg
      121 agagtgtccg gaagaaaaat tttatcgata ggaaatttta taaggcctgt taccatttct
      181 tcaacatagt cgggagaatt tcggaaaccg ttcgaccaat cctccagata aacattgact
      241 cttatcttgt tttttactgc aaagtttaca gtttcttcaa tgtcgctcaa gtgctctttt
      301 tctgtcttgt tcagctgaag agtcagatgc tttttggatc cctttgtcag gagattaagt
      361 acaccggccc cggactcttt catccattca actgttcttg tcttgtctac gaatcctaaa
      421 atttcaatct tatccgctag attttctttt tcagcccagt ttatgattgt ctttacagtg
      481 gactgctcac ctctcgaaac tctcgcagaa gccacttcca ccctgtccgc cttcagctgc
      541 agaagcagat attttgctac attgagtttt tccgcagtgc tgaaagatac tccgcgtgtc
      601 tgttcgccgt cccttaaggt tacatctaga atttctattg acatatcagt ttttatacct
      661 cttcagattc tgttttcccg gagaaagaat ttcaagcttt gttgaacctg gatttgtata
      721 aataaaatct ccgtactcgc atacccggat aaaatcaagc gtgactgcta catccaaagt
      781 cagctgcttc agtttgccct ctccaattcc gtgaactact tcgacaagcg tttcaccttc
      841 cataaaagca ttatggattt ccctttccag tttctgtttt gcatcttcat aacgaagctt
      901 tcgaatgtaa atttctctca tatacagaaa ggtacattaa aaagaagtgt accaatggcc
      961 tctaatctac taagttttga aaaggaagcg ggcagtccag cagtactttt ctggagaatc
     1021 catatttctg tactgctgag ctgaaaggag gggttatttc ttttcgaggt cttggaactt
     1081 ctgggcaatc agctgacgaa ctttggaatg ttcttcctca gtcataccgt ttttgggacc
     1141 cagactgctg tcatccattt cagagtggtt gtgcccgtaa aacttgatcc ctgctttttt
     1201 aaaatcttct tcttttacag tgattgatat gtctccctta tgaatgacta aataatctcc
     1261 actgttcact tcagacttat gagacatatt gtttgaatac tcaatctgtc cttcacaatg
     1321 acaggttccg gtcatttctc cctgattaaa tgtataaaac tttgttccgc gaacaccggc
     1381 cacagtagta ggagttttca gcttgaactc ctgatccttg gaaagtttgt ttacagatat
     1441 ccatacattt ccagcttcca gaaaaaattc ataacccttc aaagttgatt ccactttcag
     1501 ttttgaattg gattgaattt cgagctgtcc ggagatggac ccagtttcaa taatgactgc
     1561 cgatttttca ccagtcgaaa ttacgtcacc tgaattcaga acatcacctt ttttcaaagg
     1621 atgttcacct gcagaactga tgacagctgc ttttcccttt ataaatattg cggatacgga
     1681 ctgtttattt tccggcggct ttgagcaaaa actggcactg acaagcatta ctgccagaat
     1741 ttgttttttc aaaaatgagt gattcatagt gagatccttg gtttttttga atagggatta
     1801 cagcagaagc tttgctggca tctctccttt accaaaatct gagaaaaatt gtacagacgc
     1861 aatacatttt ttttaatgca ataaaaatat acagatctgt ctagttgtgt tcagcggatt
     1921 ccgatcctgg aaagaatttt ctccagctcc tcaagatctg agtaagagac gacaattctt
     1981 ccttttccat tcttttcgtt atgctggatt ttcacttttc ctgaaacttt attccggatc
     2041 ttcgattcca gttcaagtat gttcggatct gtttttgctc ctgccttttt ggactttgcc
     2101 ggagttgtaa gttttgcaac cagttcttcc acttcacgga cagtcattcc cttctttgcc
     2161 acttcttccg ccacttcaat cattttcttc ttgtcataaa gtgaaagcag aggtcttgcc
     2221 tgtccctcgc tcagtttctc tttggaaacc atttcctgca cctcggcggg aagctgaaga
     2281 aggcgtatca gatttgcgac tgtggacctg ttttttccaa gacgttcggc aagctctgtc
     2341 accttgatgg aatacttatc aataattttc tgataggcag atgcttcctc catcggattc
     2401 agatcttctc tctgaatatt ttcgagaagc gcgacttcaa tggactctct ttctgtaaaa
     2461 ttctttacta tcgcaggaat ctttacaaag cctgcctcgc ggcaggcacg aagccttctc
     2521 tctcccgcaa caagctcaaa tccttctgct gtctttttta caacaatagg ctgtatgagt
     2581 ccgtgtgctt ttattgtttc agccagctcc agaattgttt cctgagagaa attcttcctt
     2641 ggctgattcg gattcagacg tatatccgct attttgattt ctttcaaatt tgaatttgat
     2701 ccatcatccg aggagccggt atttttgctc cctccgccct ggattaaatt gccgagtcct
     2761 ctccccaata cactagtctt gctcatacat ccatccccag aaattccatt gcaaagtttt
     2821 tataacttac tgctccgacc ccatcaggat cataaaggtt gatcggtttt ccaaaggaag
     2881 gagcttctcc cagtttgata tttctcggta tcattgcctt gaataccttg tcctt
//
LOCUS       DAOQGJ010000277         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20584_length_2923_cov_3.592399, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000277 DAOQGJ010000000
VERSION     DAOQGJ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20584_length_2923_cov_3.592399"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="PK683_05630"
     CDS             <1..1887
                     /locus_tag="PK683_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07962.1"
                     /translation="GYCGPDGNTGTFIGTYNGILSFWSDSYFDMDVYVTNVSIPGSAG
                     GSPTVDAVMQVNNTNELGVTLGAKKAYVDLAVIARNRSTLGVVIPSGSRFYFTTSIEL
                     NSTSNYTLRNAIAKAGLSVNGSGNLALNLFTLQTPTNANDPNFQISQWVDNLIVGGMT
                     LQDSTSWAADLLSPITEMIANGLVPQVKAAITQSMLADILQKVAPEVLNAIVGTLSNP
                     GIDISLPSYLPAPLNSFGLNAKVQLSSDAVMRVSGANKGIVASVHAALNAKNPLAAGS
                     QRQHANGTLCSNGCFVYNKDPGTPLIDVNNHAPFNQSATVPGFQLALHSDSITQAAYH
                     MWKNRAIDLNVDSAFIASINSYAGSDPLLKLTDSLLKASAIMSIIAPGQNTLSGIDGS
                     NNLMPAICADDQVRFKIDPLMPPVVKMINNAGYDPNTNAQANVNATFSDLQVTLQGKR
                     TDNSAACMALRGAADNSYFTMATLKVSMNANANFRFVEFDNPTTGPNDYLNSLSLRIF
                     TDTLKYSIDVVEGSTANPYALDPIGIKSVFDPLVKSLVVPLVNSILNRVPLPPQVNFA
                     ALNYPATSTVCKINVQTDNYLKLNSLSVPAVDASANPYVLAQATLMGLSLGDPKYLLE
                     SSCR"
     gene            complement(1928..2557)
                     /locus_tag="PK683_05635"
     CDS             complement(1928..2557)
                     /locus_tag="PK683_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07963.1"
                     /translation="MIFLSSVFYVIAEELPKESPYSFYFYLQGGGGKMETKAETGKTD
                     TGKAGLEYRPFPFLGIGLSYSESSSGLKAKSNPLDYIIPYYLIQSELSRGQDASRSIL
                     FYSLIDSVNSRRVFNFHYNSGAVDLNFHLNGDSTFDPYIGFSFFGGSCIGKDTKCDVK
                     GGEGRLGIQFNIDSFFFYIQVQRQQTAFSGNGKIDAVSDLGSAGLGVRF"
     gene            complement(2761..>2923)
                     /locus_tag="PK683_05640"
     CDS             complement(2761..>2923)
                     /locus_tag="PK683_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNH07964.1"
                     /translation="GPWDADTEKNIISYQSPVGKALLGKKIGQTANVIFEGNATNFEV
                     LSIDPYKES"
BASE COUNT          841 a          661 c          595 g          826 t
ORIGIN      
        1 ggttactgcg gacctgacgg gaatacaggt acattcatag gaacatataa cggtatctta
       61 agtttctggt cagattccta ctttgatatg gatgtttatg tgaccaatgt ctctatacca
      121 ggctcggcag gaggatctcc tacagtagat gcagtaatgc aggtcaacaa tacaaatgaa
      181 cttggtgtaa ctctcggtgc caaaaaagct tatgtagact tggctgtaat cgccagaaac
      241 agaagcactc ttggggttgt aattccttcc ggaagcaggt tttattttac aacaagcatt
      301 gagctgaaca gcacatccaa ttacactttg cgaaatgcaa ttgctaaggc tggactttct
      361 gtcaatggat caggaaactt ggcactgaat ctgtttacat tgcagacccc tacaaacgcg
      421 aatgatccta acttccagat ctctcagtgg gtagacaacc tgattgtagg aggaatgaca
      481 cttcaggatt caacaagctg ggcagcggat ctcctctctc caatcaccga aatgatcgca
      541 aatggattag tccctcaagt caaagctgca atcactcagt caatgcttgc agacatactt
      601 caaaaagttg ctcctgaggt gctgaatgct attgtgggaa ctctttccaa tcctgggatc
      661 gatatctctc ttccaagtta tctgccggct ccattgaatt cattcgggct gaatgcaaaa
      721 gtgcagctct ccagtgacgc agtaatgaga gtgagcggag caaataaagg gattgtagcg
      781 tccgtacatg ccgctctcaa tgcaaaaaac ccattggcgg ccggttcaca gagacagcac
      841 gcaaacggaa cactctgttc caacggatgt tttgtgtata ataaggatcc aggcacgcct
      901 ctcattgacg taaacaacca tgcaccgttc aatcaaagtg cgacagttcc aggatttcag
      961 ctcgcacttc attcggattc catcactcag gctgcatacc atatgtggaa aaacagagca
     1021 atcgacctga atgtggacag tgccttcatt gcatccatca acagttatgc aggttctgat
     1081 cctcttctga aactgactga ctcacttttg aaagcatcgg ccattatgtc gatcatagct
     1141 ccgggacaga atactctctc aggaatagac ggatcaaaca acctgatgcc tgccatctgt
     1201 gcggatgacc aggtcagatt taaaattgat cctctgatgc cgcccgtagt gaaaatgatc
     1261 aacaatgcag gttatgatcc aaacacaaat gcacaggcaa acgtaaatgc tacattcagt
     1321 gatcttcagg ttactcttca ggggaaaaga acagacaaca gtgctgcctg tatggctttg
     1381 agaggagctg cagacaacag ctactttaca atggctacac tgaaagtcag tatgaacgca
     1441 aatgcaaatt tcagattcgt agaatttgac aaccccacta caggtccaaa tgactatctg
     1501 aattcactca gcctcaggat ttttactgat actctgaaat acagcattga tgttgtagaa
     1561 ggatcaaccg caaatccgta tgctcttgat ccaatcggaa tcaaatcggt gtttgaccca
     1621 ttagtcaaat cattggtggt tcctttggtg aacagcattc tgaacagagt tcctctgcct
     1681 cctcaggtaa atttcgctgc actgaattat cctgcgacaa gcacagtctg caagattaat
     1741 gttcaaacgg acaattatct gaaactgaac agtttaagtg ttcctgctgt ggatgcttca
     1801 gcgaatccgt atgttctggc tcaggcgact ttgatgggac tttctcttgg tgacccaaaa
     1861 tatctgcttg agtcaagctg cagataatta ctaaaggcgt gaatcagtaa tggttcacgc
     1921 tgttttttta aaatcttact cctagtccgg cacttcccaa atcagatact gcgtcaatct
     1981 ttccatttcc tgaaaaagcg gtctgctgcc gctgaacctg aatgtaaaaa aagaaactgt
     2041 caatattgaa ttgaatgcct agcctgccct cccctccctt cacatcacat tttgtatcct
     2101 ttccgatgca gcttcctccg aagaaactga atccgatata tgggtcaaat gtactgtctc
     2161 cgttcagatg gaagtttaaa tcaaccgctc ctgaattata atgaaaattg aatactcttc
     2221 tggagttaac tgagtcgatt aatgaataaa aaaggatgct tcgagatgcg tcctgtccgc
     2281 gagaaagttc tgattgaatt aaataatatg gaattatata gtctaacgga ttgctttttg
     2341 ccttcagacc ggaactgctt tcagaataac tcaatccgat ccctaaaaaa gggaatggtc
     2401 tatattccag accggccttt ccggtatctg ttttgccggt ctctgctttc gtttccatct
     2461 tccctccgcc cccctgcaga taaaaataaa atgaataggg ggattctttt gggagttcct
     2521 ctgcaatgac ataaaacaca gaagataaaa aaatcataaa aaatttcata attctgcaga
     2581 tattatctct tttgtacgat gattggtgtt ttgttcagat aatgaggttg tgtaaaagca
     2641 tacctcgata catttttcac cgccggttac cgagtgaaaa acccaatatt ttcagggttt
     2701 ttcgtatcga aaatcactcg gtaacctgct tttacacagt cccatttgat tacttatttt
     2761 tcagctttcc ttgtaaggat ctatagacag aacctcaaag ttagttgcat tcccttcgaa
     2821 gatgacattg gcagtctggc cgattttctt tccaaggagt gcctttccta caggagactg
     2881 ataactgata atgttctttt cagtatctgc atcccatggg ccg
//
LOCUS       DAOQGJ010000278         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20588_length_2923_cov_2.561715, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000278 DAOQGJ010000000
VERSION     DAOQGJ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20588_length_2923_cov_2.561715"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PK683_05645"
     CDS             complement(<1..394)
                     /locus_tag="PK683_05645"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397555.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNH07965.1"
                     /translation="MKVLLIGSGGRESAIAWQIRKSPLLSLLKVYPGNAGFPDSEIVL
                     DGPNLKSKESVQNFVRKEKFDFVIAGPEEPLVDGICDWLSEMGIPCFGPSAYCAQLEG
                     SKDFAKKLMKDFGIPTAEYSTFTDYHSAI"
     gene            complement(391..>2923)
                     /locus_tag="PK683_05650"
     CDS             complement(391..>2923)
                     /locus_tag="PK683_05650"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNH07966.1"
                     /translation="GEPFSIVIPPPNVTGTLHIGHALNHTLQDIIIRIERKKGKDALW
                     LPGMDHAGIATQVVVERELAKKGKKRTDFSREEFVKQVWDWKKFSGGEITKQQKLLGE
                     SVDWKRERFTFDAGLSKAVIRVFKRLYDEGLIYRGERIINWCPKNLTAISDIEVEFKE
                     VHGHLYHIRYPISGTKEHIIVATTRPETMLGDVAVCAHPEDNRYAHLKGKNLLLPIMN
                     REIPLLFDSFVDKEFGSGLVKITPAHDPADFEAGQRLGLKPINIMNPDATLNENAGKY
                     KGYDRFDARKAVVEELKSLGLIEKIEDYTHSVGHNSRGGAVIEPYLSTQGFVKVKPLA
                     EKAIEAVRSGKTEFVPKMWEKTYFEWMENIKDWCISRQLWWGHRIPAYYCSDCGHMEV
                     SETEIKKCPSCKSEKVKQDDDVLDTWFSSGLWPFTTMGWPDDTADVKKFYPTSVLVTG
                     FDIIFFWVARMMMFGLKFMNEVPFRKVVIHGLVRDRHGKKFSKSMGNTIDPLDMMSKY
                     GTDSFRFFLAASLPEAKDILFDESRLEGYRAFCNKIWNSSRFILMNLPEDFRTEAVPE
                     KDLESSDLWIQSRFNVCLEKYEKAYSGFHFYEMAALIYDFIWGDFCDWYLELTKPRIY
                     GKSTEKSKSAALQTLADILIKALNLLHPFMPFLTEEIHSAFSEELMTASPWVKPFNVK
                     ADSEKVQKIEMLTEIITKVRTVRSELNVPPDRKCRIIIKSDKSLVAEMIHEEQNSLLQ
                     LSRAESAVLNKEHKTDRTDSVTAFRFGEIILPLSGLVDMEKEKQRLKKEMDSVAAELE
                     KINKKLNNPEFISKARPEVISKEKEKAEVLKNKAASLEKALERYK"
BASE COUNT          702 a          695 c          626 g          900 t
ORIGIN      
        1 ctattgcaga atgataatca gtaaacgtac tgtattctgc agtaggtatc ccgaaatcct
       61 tcatcagctt tttggcaaaa tccttggagc cttccagctg tgcacagtag gccgagggcc
      121 cgaaacaggg aatgcccatt tctgagagcc agtcgcagat tccgtccacg agcggctctt
      181 ccggaccagc tatgacaaaa tcaaatttct cttttctgac aaagttctgc actgattcct
      241 tggatttcag gttcggtccg tccagtacga tttctgaatc aggaaaacct gcatttccag
      301 gatatacttt cagcagggaa agaagaggcg attttcttat ctgccaggca attgcggatt
      361 ctctgccccc gcttcctatc agaagtacct tcatttgtat ctctccagtg ccttttcaag
      421 cgaagcagcc ttgtttttca gaacttcagc cttttctttt tccttggaaa tgacttcagg
      481 tctggccttt gatataaatt caggattatt cagtttttta ttaatttttt ccagttcggc
      541 tgcaactgag tccatctctt ttttaagtct ctgtttttcc ttttccatat ccacgagacc
      601 tgaaagggga agaatgattt caccgaaacg gaacgcggtc actgaatctg tcctgtccgt
      661 cttatgctcc ttgttgagga ccgcagattc tgctctcgag agctgaagaa gggaattctg
      721 ctcttcatga atcatttctg ccacaagaga cttgtccgac ttgatgatga ttctgcattt
      781 tctgtccggc gggacattca gttcagaacg cacagtcctg accttcgtga tgatttcagt
      841 gagcatttca attttctgaa ccttttcaga gtctgccttg acattgaacg gttttaccca
      901 gggagaagcg gtcataagtt cttcagagaa tgcagaatgg atttcctcag tgagaaacgg
      961 cataaatgga tgcagcagat tcagtgcctt gatgagaatg tctgccagag tctgaagtgc
     1021 tgctgacttt gatttttctg tggactttcc gtaaattctc ggtttggtca gttccagata
     1081 ccagtcgcag aaatctcccc agatgaaatc atagatcaaa gccgccattt cataaaaatg
     1141 aaatcctgaa tatgcctttt catatttttc cagacatacg ttgaatctgc tctgaatcca
     1201 gagatcggaa gattccagat ctttttccgg aacggcctcc gttctgaaat cttcaggaag
     1261 attcatcaga atgaaacggc tgctgttcca aatcttgttg cagaaggctc tgtagccttc
     1321 cagtctgctt tcgtcaaaca ggatatcctt agcttccgga agacttgctg caagaaaaaa
     1381 acggaatgag tcagtcccgt atttggacat catatccaga ggatcaattg tattgcccat
     1441 ggatttgctg aatttttttc catgtctgtc ccgaaccagt ccatgaatga ctacctttct
     1501 gaagggaacc tcattcataa atttcagtcc gaacatcatc attctggcca cccagaagaa
     1561 aataatgtcg aatccggtga caagtacaga agtagggtag aactttttga cgtcggctgt
     1621 gtcatcaggc cagcccattg tcgtaaaagg ccaaagtcct gaagagaacc atgtgtcgag
     1681 cacatcgtca tcctgtttta ccttttcaga tttgcaggaa ggacatttct tgatctcagt
     1741 ctcagagact tccatatggc cgcagtcaga acagtagtat gctggaatcc tgtgtcccca
     1801 ccagagctga cgggaaatac accagtcctt gatgttttcc atccattcga aataagtttt
     1861 ttcccacatt ttgggcacaa actcagtctt tcctgaacgg accgcttcga tcgccttctc
     1921 tgcaagaggc tttactttta caaacccctg tgtggaaaga tacggctcga tgactgcacc
     1981 gcctctgctg ttgtgaccta cggagtgagt atagtcttca attttttcga taaggcccag
     2041 gcttttaagt tcctctacta ctgcctttct ggcatcaaat ctgtcgtaac ccttgtattt
     2101 gcctgcattt tcattcaaag tcgcgtcagg gttcattata ttgatcggtt tcagtcccag
     2161 tctctgtcct gcttcaaagt cggcaggatc gtgggcaggg gtgatcttga caagacctga
     2221 tccgaattcc ttgtccacaa aactgtcgaa aaggagaggg atttcacggt tcataatcgg
     2281 caggagcaga ttttttccct tgagatgagc atatctgtta tcttccggat gagcacagac
     2341 tgccacgtcg ccgagcatag tttcgggtct tgtggtcgcc actatgatat gttcttttgt
     2401 gcctgagata gggtaccgga tatggtataa atgaccgtgg acttccttga attccacttc
     2461 tatgtccgaa attgcagtca ggttcttagg acaccagttg atgatccttt cccctctgta
     2521 gatcagtcct tcgtcataca ggcgcttgaa tacgcggatt acagctttcg acaaccctgc
     2581 atcgaaagtg aagcgttctc ttttccagtc cacgctttct ccgagaagtt tctgctgttt
     2641 tgtaatttcc ccgcctgaaa actttttcca gtcccagacc tgttttacga attcttctct
     2701 tgaaaaatca gttctttttt ttcccttttt ggccagctct ctttctacga cgacctgagt
     2761 tgcgattccg gcatgatcca ttcccggaag ccagagagca tccttgcctt tttttctttc
     2821 aatacggatg atgatgtcct gaagagtgtg attcagtgca tgtcctatat ggagtgtgcc
     2881 tgtcacgttc ggaggaggaa ttacgatgga aaagggttct cct
//
LOCUS       DAOQGJ010000279         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20601_length_2922_cov_3.046390, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000279 DAOQGJ010000000
VERSION     DAOQGJ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20601_length_2922_cov_3.046390"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK683_05655"
     CDS             complement(<1..220)
                     /locus_tag="PK683_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017854419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNH07967.1"
                     /translation="MMKEKIIVAMSGGVDSAVTAGLLLEQGYDVIGVNLRTWEYEPPA
                     CDTTKKSCCSPEDIQDARDTGLSFKIPFY"
     gene            complement(223..1035)
                     /locus_tag="PK683_05660"
     CDS             complement(223..1035)
                     /locus_tag="PK683_05660"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNH07968.1"
                     /translation="MSGIWLNYINWTPVVELKAEPAEKGIAPPVHLRFLLDTGSNTTL
                     LRKDFIPGVEKQVYSSFTTHGKTELELHPYEVRLSSPDEQEIFKGTVNLIKFEENLPF
                     DGILGNDVLSHYILVLDHFYGAFLEKRQILKKNWFRQMKKIQLNANAPRHLMVSALAG
                     KNLERMDMLIDTGANHTYLGSETAENLKLKLIDKKPYTDIRGVEQEGKIFRLENLCIE
                     KFCKKEYPVLSGSSLRGFLGEDGIPVAGLIGYDWMREHVIAVDYLLHEVYIR"
     gene            complement(1316..2014)
                     /locus_tag="PK683_05665"
     CDS             complement(1316..2014)
                     /locus_tag="PK683_05665"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HNH07969.1"
                     /translation="MNFLIFLANCPWDGFAPPTIKYCENNLCSWITQPSNTWSNLIFY
                     IAGILLFRNIQNRNNSGPQTSFLYINAFLIGTASFLYHASFTFLMQFFDLSSMYMFSV
                     TLLCLNFYRMGLVRKKEIFTVFLLVLAVCMGALYFLKPYGIPIFALTAAAAIGTEFII
                     FRNGREKADYSRYKIGLILILSAFLFWALDFRRIWICDPDNHLFQGHAVWHILSGAAY
                     YQFAMFFIDLQESE"
     gene            complement(2011..2535)
                     /gene="msrA"
                     /locus_tag="PK683_05670"
     CDS             complement(2011..2535)
                     /gene="msrA"
                     /locus_tag="PK683_05670"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016749986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNH07970.1"
                     /translation="MKTEKIILGGGCFWCLEAVYLQAEGIISAVSGYSGGHRENPTYR
                     EVCEETTGHAEVVLLEFEPEKISLESILEIFWLIHDPTSLNRQGADEGTQYRSAVYYM
                     EDFQKETVMNSVQNQEKVIGKKIVTEIRKADKFYPAEDYHKNYYNLNPETSYCRYVIR
                     PKLEKYIQFRKKAK"
     gene            complement(2535..>2922)
                     /locus_tag="PK683_05675"
     CDS             complement(2535..>2922)
                     /locus_tag="PK683_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07971.1"
                     /translation="LVFAAALLCFFSSEVLMRLSYCRSCFLSETQNWLLHTGLVSDCF
                     IFGFLLQWIAEKYSGRMPRGFGIVLALGAVFAALLSLITVKLFPGYGNGADWVDAVKM
                     GYPVFYSVLLTGLSSLWASLKLSGET"
BASE COUNT          836 a          639 c          605 g          842 t
ORIGIN      
        1 cataaaaggg tattttaaag gaaagtcctg tatcacgggc atcctgaatg tcttcaggag
       61 aacagcagga ttttttagtc gtgtcgcagg ccggaggctc gtattcccag gtcctgagat
      121 tgaccccaat tacatcatat ccctgttcca gaagaagacc tgcggtcaca gcactgtcca
      181 ctccgccgct cattgcaaca atgatttttt ctttcataat aattaccgga tatatacctc
      241 gtgcagaaga tagtccactg caatcacatg ttcccgcatc cagtcgtagc cgatcaggcc
      301 tgcgactgga attccgtctt ctcctaaaaa tcccctgaga gaagatccag aaagtacagg
      361 gtactccttt ttacagaatt tttctatgca gagattttcc aaccggaaaa tttttccttc
      421 ctgttcaacg cctctgatat ccgtataagg ttttttatca ataagtttta atttaaggtt
      481 ttctgccgtt tctgaaccca gataagtatg atttgctcct gtgtctatca gcatatccat
      541 tctctcgaga ttctttcccg caagagctga taccatcagg tgccttggag catttgcatt
      601 cagctgaatt tttttcatct gcctgaacca gtttttttta aggatctgtc ttttctccag
      661 gaaggctccg taaaaatggt caagaaccag aatataatga gagagtacgt cgtttcccag
      721 gattccgtca aaaggaaggt tctcctcaaa cttgatcaga tttactgtgc ctttgaatat
      781 ttcctgttca tcaggagaac tcagtctgac ttcatacgga tgcagttcca gttctgtctt
      841 cccgtgagta gtaaatgaac tgtaaacctg tttttctact ccggggataa aatctttcct
      901 gagcagagtg gtgttggatc ccgtatcaag aagaaatcgt aagtgtacag ggggagcaat
      961 tcccttctca gccggttccg ctttgagttc cactaccggt gtccagttta tataattaag
     1021 ccatatcccg ctcaaattct gtgctctgat gaaaaaactg ttcctgcatc cggttgaaaa
     1081 aatcagtaca aaaatacaca gtatcctgaa ataatgaaag ctgtgttttt ttgggaagca
     1141 cttctgctgt tctgaaagaa agactgatga aaacacggga aaattatcag aaacgcgcaa
     1201 ggtaagggta tctgcttctg ctgactttaa attcaaaaga accataactc aatgatgcag
     1261 tcttcaatct caatttcaat tagaattctg cccgagagtt ctgaaaaaga gaaatttatt
     1321 cagattcctg aaggtcgatg aagaacattg caaactgata atatgcggct cctgaaagaa
     1381 tatgccatac tgcatgtcct tgaaacagat gattgtccgg atcacagatc cagattctcc
     1441 tgaagtccag tgcccagaaa aggaaggcag aaagaataag aatcaggccg attttgtatc
     1501 ttgaataatc tgctttctcc ctgccatttc tgaagatgat gaattctgtt ccgattgctg
     1561 cggcggcagt aagtgcaaat ataggtatcc cgtatggttt cagaaaataa agtgctccca
     1621 tacagacagc cagaaccaga agaaagactg tgaagatttc cttttttctc acaagaccca
     1681 ttctgtaaaa gttcaggcag aggagggtga cagaaaacat gtacatcgag gaaagatcga
     1741 aaaactgcat cagaaaagta aatgaggcat gataaagaaa agatgccgtt cctatcagaa
     1801 aagcattgat gtaaagaaaa ctcgtctgag gaccggaatt atttctgttt tgtatgtttc
     1861 tgaacagaag tatccctgca atatagaata tcagatttga ccaggtgttg gacggctgag
     1921 taatccagga gcagagattg ttttcgcagt actttattgt aggaggcgca aatccgtccc
     1981 aggggcagtt tgccagaaaa atcagaaaat tcatttggcc tttttcctga actggatgta
     2041 cttttcgagt tttggacgaa tcacgtatct gcagtaggaa gtttccggat tcagattata
     2101 ataattttta tgataatcct ctgcaggata aaacttatcc gcttttctga tttcagtcac
     2161 gatttttttt ccgatgactt tctcctgatt ctgaactgaa ttcatcacag tttctttctg
     2221 aaagtcttcc atataatata ctgcagatct gtactgagtt ccttcgtccg ctccctgcct
     2281 gttcagacta gtcggatcgt gaatcagcca gaaaatttcc agaatggatt ccagactgat
     2341 tttctcaggt tcaaattcca gcagtacgac ttctgcgtgt ccagtggttt cctcgcagac
     2401 ctctctgtac gttggatttt ctctgtgtcc tccggaatat ccggatactg cggaaatgat
     2461 tccttctgcc tgaagataga ccgcttcgag gcaccagaag catcctccgc caaggatgat
     2521 tttttctgtt ttcattatgt ttctcctgac agttttaatg aggcccataa ggatgaaagt
     2581 cccgtgagca gtacggaata aaatactgga tatcccattt tcactgcatc aacccagtct
     2641 gctccgtttc cgtatccggg aaaaagcttc actgtgatca gactgagaag cgcggcaaag
     2701 accgctccca gagcaagtac aattccgaag cctcgaggca tcctgcctga atatttttca
     2761 gcaatccact gcagcaggaa tccgaaaata aaacagtccg ataccaagcc tgtgtgcaga
     2821 agccagtttt gagtttctga cagaaagcag gatctgcagt aggaaagtct catcagcacc
     2881 tcgcttgaga agaagcataa caaagctgcg gcaaaaacaa gg
//
LOCUS       DAOQGJ010000280         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20670_length_2916_cov_2.708843, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000280 DAOQGJ010000000
VERSION     DAOQGJ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20670_length_2916_cov_2.708843"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PK683_05680"
     CDS             complement(<1..141)
                     /locus_tag="PK683_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980422.1"
                     /note="seems to be involved in biofilm formation; in
                     asymptomatic bacteriuria E. coli strains 83972 and VR50,
                     the ygiD gene is upregulated during biofilm formation in
                     urine; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNH07972.1"
                     /translation="MMPVLFMGHGSPMNAVEDNEYTRAWKNIAKEIQKPKAILCISAH
                     WLT"
     gene            complement(150..1886)
                     /locus_tag="PK683_05685"
     CDS             complement(150..1886)
                     /locus_tag="PK683_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNH07973.1"
                     /translation="MDSKAIKHRRDFLKKCPLFAKVKPLTLQKLAELVIERKVHNKEA
                     IFTAGEPSDKLFIVRYGEVMLKYGSGEVFMSPGDALAEISLVTKLPHSSTAEASMDCL
                     MYEINGEKFLRLSESDRQLSQNLITMISSRFRENILTKNEKTYPRRFICHVKFDAIPD
                     FNAEVTRVVKSCEQAAGRKSIVLNPDSFKGLNQEKILKFLSENRQKYPVIHLKLDRIN
                     DFFMFEHIASHADYIIFYENEVNLAWTNKLDFFRKIRTDIRNFEGRGIRYIQERNTMY
                     PAGESSAEKIFYNRELLSRFIVSRTRGLTLGGGGARALAHVGLLRVLEKEKIHFDFVS
                     GSSMGAVVGALYARGDMVSEIEKTVRRFFGGLESPFDPTIPLISFYKGKKMKKMLKEV
                     FRDERIEDYRIPFVTSAIDIQTGEEYVFDKGPLWEALLCAMSLPAVFPPVFLGEHLLV
                     DGGTLNNVPDNLIRNKGADRILSVNVSPLKDRGLYQLLENRKETGKSLFKSLWEYIKY
                     PPILKIMGRANMLEGREITKARLSRMDCFLHFHLEEFNLFDFRRYDEIINKGESEAEK
                     HLDEIKRLFFPS"
     gene            complement(1953..>2916)
                     /gene="gcvT"
                     /locus_tag="PK683_05690"
     CDS             complement(1953..>2916)
                     /gene="gcvT"
                     /locus_tag="PK683_05690"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011672071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNH07974.1"
                     /translation="FDVSHMGEIFLEGSEKELLELLERLTCNTVSRMHDGQVQYNAVV
                     NENGGLVDDITVYRFSAGKFMICSNASNYEKVYEHFLKYNSSSVQIRNESSSWHQIAI
                     QGPKADHMFSDYIGQDISHIKYYHFAPVKFRGEEMIVSRTGYTGEDGFEIYSSVSLGL
                     SIWSEILEMKKKDGLVPVGLGARDTLRLEAKYPLYGHELNETRTPVESGIGWIVKEKD
                     APYLGYDRIINDKKNGAKVSLYAMILKEPGVLRENFKVFDGSGNEIGLTTSGSYSPSL
                     RQSIALAVLRTGIPDGTEVFAEIRGEKKKAAIHQSKFIPGSVKK"
BASE COUNT          780 a          699 c          561 g          876 t
ORIGIN      
        1 ggtcagccag tgagcagata tgcagagaat tgccttcggc ttctgtatct cttttgcaat
       61 atttttccat gctctggtat actcgttgtc ttccacagca ttcatagggg aaccgtgtcc
      121 cataaacagt acaggcatca taggcaatat taggaaggaa agaaaagtct cttgatctca
      181 tcgagatgct tttctgcttc tgattctcct ttatttataa tttcatcata tctgcgaaaa
      241 tcaaaaagat tgaactcctc cagatgaaaa tgcaggaaac agtccattct cgaaagtctg
      301 gccttggtaa tttctcttcc ttccagcata tttgcccttc ccataatctt tagaatcgga
      361 ggatatttta tgtactccca aagactcttg aaaagagact ttcctgtctc ttttctgttt
      421 tcgagaagct gatacagtcc cctgtccttc agaggggaaa cattgactga cagaattctg
      481 tctgcaccct tgtttcgaat caggttgtca ggcacattgt tcagtgtgcc cccgtccaca
      541 agaagatgct cgccgagaaa aacaggaggg aaaaccgcag gaagactcat cgcacacaaa
      601 agtgcctccc aaagaggtcc tttgtcaaag acatactctt ctcccgtctg aatatcgatg
      661 gctgacgtca caaagggaat acggtaatct tcaattcgtt catctctgaa aacctccttg
      721 agcatcttct tcattttttt tcccttataa aacgaaatga gcggaatggt aggatcgaaa
      781 ggactttcca gtcccccgaa aaatcggcgg acagtctttt caatttcgga aaccatatct
      841 cctcttgcat agagtgcgcc tacaactgct cccatggaag atcctgatac gaaatcgaaa
      901 tggatttttt ctttctcaag cacgcgcaga aggcctacat gggcaagagc tctggcacct
      961 ccgccgccta gagtcagacc tcttgtacga gatactatga atctcgaaag cagttccctg
     1021 ttgtaaaaaa ttttctctgc agaagactcg cctgcaggat acattgtatt tctttcctga
     1081 atataacgga tgcctcttcc ttcaaaattg cggatgtctg tccttatctt ccggaaaaaa
     1141 tccagcttgt ttgtccaggc cagattcacc tcattttcat aaaatattat ataatccgcg
     1201 tgacttgcaa tatgctcaaa catgaaaaaa tcattaattc tgtccagctt cagatgaatc
     1261 acggggtatt tctgacggtt ttcactcaag aatttcagga tcttttcctg attcagtccc
     1321 ttaaaactgt caggattcag tacgatggat tttctgccgg ctgcctgttc gcaggatttt
     1381 accactctcg tcacttctgc attaaaatca ggaatggcat caaacttcac atgacagata
     1441 aatcttctgg gataagtttt ttcattcttg gtcagaatat tttcacggaa ccgtgaagag
     1501 atcatagtaa tcagattctg agagagctgt ctgtccgatt ctgaaagtct gaggaatttt
     1561 tcaccgttga tctcatacat cagacagtcc atggaagcct cggcagtgct ggaatgagga
     1621 agctttgtca caaggctgat ctcagcgagc gcatctcctg gactcataaa gacttcaccg
     1681 ctcccgtact tcagcatcac ctcgccgtac cggacaatga aaagtttatc tgaaggctcg
     1741 cctgcagtga aaatcgcttc cttattgtgc actttgcgct cgatgaccag ctcggcaagc
     1801 ttctggagtg taagcggctt gaccttcgca aacaaaggac attttttcag aaagtccctt
     1861 ctgtgtttaa ttgccttgga atccatgcta ggcaaagatt tcagccaagt aatctaaaat
     1921 caagaagatt tcagggttca ggctgattat ccttatttct tcacgcttcc tggaatgaat
     1981 ttgctctggt gaattgccgc ctttttcttt tctcctctga tctctgcaaa aacttcagtt
     2041 ccgtcaggaa taccggtcct gagaacggca agagcaatgc tctgacgaag cgacggtgaa
     2101 taagaaccgg aagtggtcag tccgatttca tttcctgatc cgtcaaacac cttgaaattt
     2161 tctctgagaa ctccaggttc tttcagaatc attgcataaa ggctcacctt cgctccgttt
     2221 tttttatcat taataatcct gtcatatccc agataagggg catctttttc ttttacaatc
     2281 cagccgattc ctgactccac cggtgttctg gtttcattca gttcatggcc gtagagagga
     2341 tactttgctt caagtctcag ggtgtctctt gctcccagtc ctacaggcac aagaccgtct
     2401 ttttttttca tttccagaat ctcgctccag atggacagtc caagagaaac agaactgtaa
     2461 atctcaaatc cgtcctcgcc tgtatatcct gtccgagata caatcatttc ctcccctctg
     2521 aatttcacag gagcaaaatg ataatacttt atatgagaaa tatcctgtcc tatataatca
     2581 gaaaacatat gatctgcctt ggggccctga atcgctatct ggtgccagga actgctttca
     2641 tttctgatct gaacggaact gctgttgtat ttaagaaaat gctcatatac cttttcatag
     2701 ttggatgcat ttgaacagat cataaacttg ccggcgctga atctgtatac agtgatatca
     2761 tccacaagac cgccattttc attgacaact gcattgtact gaacctgtcc gtcatgcatt
     2821 ctggaaacag tattgcaggt cagtctttca agaagctcca gaagttcctt ttcactgcct
     2881 tccaggaata tttctcccat atgagaaaca tcaaaa
//
LOCUS       DAOQGJ010000281         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20794_length_2906_cov_2.781129, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000281 DAOQGJ010000000
VERSION     DAOQGJ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20794_length_2906_cov_2.781129"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PK683_05695"
     CDS             complement(<1..334)
                     /locus_tag="PK683_05695"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07975.1"
                     /translation="MKKLPIGIQTFSKIREGNYYYVDKTQFLADLVENGSYYFLSRPR
                     RFGKSLFLDTLREAFLGSKEYFKGLYLENNWNWDKKYPVIRIDFGTGQLRSASELEQR
                     ILEILMNNR"
     gene            546..1769
                     /locus_tag="PK683_05700"
     CDS             546..1769
                     /locus_tag="PK683_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNH07976.1"
                     /translation="MEFLQNHSQLIGILLMPLTYGFTGWFTNVVALKMTFYPLNFIGI
                     PPYLGWQGIVPRKATGLALKAVQMLTERLVRVEEFFGKLDPEMIRKNFEPVILKEVPP
                     AVDQMVNSLEDKYRNLINEEDRKKIVLLAEEEAVSKVKELTVLLKENANKVFNLKTVV
                     LKNLTGKNVSIIVDIFQGVGKKEFKFIERSGWYFGAALGILQMGFWYLYPQWWTLPVQ
                     GVIVGYITNWLALYMIFRPLYPKKFLFLSYHGLFLKRQEEVSREYSRMFATHVLTPKN
                     IMEEVLYKRTARAVIEAIEDQTGKIIREKSGDTSSDLIEILDKETGKIQPKLMEEMIG
                     MLSASSSTMEKLISRSMNVEESMFQKMKVLPPEEFEPILRTAFQEDEFILILIGSVLG
                     ALVGLMQAVYMIYLG"
     gene            complement(1757..2056)
                     /locus_tag="PK683_05705"
     CDS             complement(1757..2056)
                     /locus_tag="PK683_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH07977.1"
                     /translation="MPILWRLREKTMRYSELKNDLRNISHKMPASPLREPEENGFVDR
                     KVYPVSPPKTEYSLTSRGKKYLPVIEALRKYGNELKKDLKGMRGRLRSILRRHPR"
     gene            2159..2902
                     /locus_tag="PK683_05710"
     CDS             2159..2902
                     /locus_tag="PK683_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNH07978.1"
                     /translation="MSSNLNIMPGRKKKIALVAANPSVSKTTGWPIGFWWSELTHPYW
                     EFTEKGFEVEIFSPEGGTLQADGYSDPEDQSGYSAHDIISLGFKKSAKHLALIENTKK
                     LSLLKVEDFDAVFLTGGQSPMYSFIDNAELHKVFVSFYEKGKVAAAICHATCILLKAK
                     TSDGRLLAEGKTWTGFADSEEQYADNFVGQKIQPFWIESEAKKIPGTNYINGGLFREF
                     AVRDGNLITGQQQFSGAAAARLVIEALGV"
BASE COUNT          835 a          580 c          685 g          806 t
ORIGIN      
        1 ttcgattatt catcaaaatt tccagaatcc tctgctccag ttcagaagca gaacggagct
       61 ggcctgttcc gaaatctatc cgaatgacag gatatttttt atcccagttc cagttgtttt
      121 ccaggtacaa gcctttgaag tattctttac tgccgagaaa agcctctctc aatgtatcca
      181 gaaaaagact ttttccgaac ctgcggggcc gggacagaaa ataataggaa ccgttttcca
      241 ccaaatcagc aaggaactgg gtcttgtcca catagtaata attgccttcc cgaattttgg
      301 aaaaagtctg aatgccgatc ggaagtttct tcacagttga aaagtctcaa agcatcccgg
      361 cctgtcaaac aaatcctttt ttgggaattt acagaatcgg ttctgttttt tgaatgtaac
      421 ggagcagatt taagactgca gtgcagcccc ttcggaaaaa ggtgtcatct gagcctgaca
      481 ggttcggaac gctggggaaa aatttgcttt actatttaac actgttaaat ttagggtacg
      541 catatatgga atttttacag aatcattcac aattgatcgg cattcttctg atgcccctca
      601 cctacggatt cactggctgg tttacaaacg tagtcgcact gaaaatgacg ttttatcccc
      661 tgaatttcat cggaattccg ccgtatctgg gctggcaggg gattgtgccc cggaaggcca
      721 ctggactcgc cctgaaggcc gtacagatgc ttacagagag gcttgtgagg gtggaagagt
      781 tcttcggaaa actggatccg gagatgatcc gtaaaaattt cgaaccggtg attttgaaag
      841 aggttccccc tgcagtcgat cagatggtga attctctgga ggacaagtac agaaacctga
      901 tcaatgaaga ggacaggaaa aagattgtgc tcctggcaga agaagaggct gtctctaaag
      961 taaaagaact tacggttctt ctgaaagaaa atgcgaacaa ggtattcaac ctgaagacag
     1021 tggttctgaa aaacctcacc ggtaaaaatg tcagcatcat cgtggatatt tttcagggag
     1081 taggaaaaaa ggaattcaag ttcattgaaa gaagcggatg gtacttcggg gcagctctgg
     1141 gaattctgca gatgggtttt tggtatctgt accctcagtg gtggaccctt cctgtgcagg
     1201 gagtgattgt aggttatatt actaactggc ttgcactgta tatgattttc aggcctttat
     1261 atcccaaaaa atttcttttt ctctcctatc acggtctctt tctgaaaaga caggaagagg
     1321 tttcgagaga gtattccaga atgtttgcga ctcatgtcct gactccaaag aacataatgg
     1381 aagaggttct gtataaaaga actgcaagag cagtgattga agcaatcgaa gaccagaccg
     1441 gaaaaatcat ccgtgagaaa agcggagaca cttcttcgga tctgattgaa attctggaca
     1501 aagaaacagg aaagatccag ccgaagctga tggaagaaat gatcggaatg ctttctgcaa
     1561 gttcctccac tatggagaaa ctgatttcca gaagcatgaa tgtggaagaa tccatgttcc
     1621 agaaaatgaa agtccttccg cctgaagaat tcgaaccgat tctccgcaca gcttttcagg
     1681 aagatgaatt catactgatc ctgatcggtt ctgttctagg tgcccttgtg ggactgatgc
     1741 aggcagtgta tatgatttat ctgggatgac ggcgcaggat tgaccttaac ctacccctca
     1801 tccccttcag gtcctttttc agttcgttcc cgtatttccg aagggcttca atgacaggca
     1861 ggtatttttt tccgcgtgag gtcagactgt actctgtctt gggaggactc acaggataga
     1921 cctttctgtc tacaaagccg ttttcttcag gctcacgaag cggacttgca ggcattttat
     1981 gactgatgtt ccgcaggtca ttcttgagtt cactgtatct cattgttttt tctctgagtc
     2041 tccagaggat tggcatttaa ctttccttta tgatagtatg gttctttttg tattatatag
     2101 attgacatgg attatttaaa aaaaaagctg ttctgtaaat taatttactg gagtttttat
     2161 gagttccaat ctgaacataa tgcctggaag aaaaaagaaa attgcacttg tcgcagcaaa
     2221 tccatccgta tccaaaacta caggatggcc tatcggattc tggtggtcgg aactgactca
     2281 cccttactgg gagtttactg aaaaagggtt tgaggtggag attttttctc cggaaggagg
     2341 tacacttcag gcagacggct acagtgatcc ggaggatcag agcggttatt cagcgcatga
     2401 tattatatcc ctcgggttca aaaagtctgc aaaacatctt gcactgattg aaaatacgaa
     2461 gaaactttcc cttctgaagg tggaagattt tgatgcagtc tttctgacag ggggacaaag
     2521 tcctatgtac agtttcattg ataatgcgga gcttcataaa gtatttgtat ctttttatga
     2581 aaaaggaaaa gtcgccgctg ccatctgtca tgcgacctgc attcttctga aagcgaaaac
     2641 atctgacggc agacttctgg ctgaaggaaa aacctggaca ggatttgcgg acagtgagga
     2701 acagtatgca gacaattttg tgggacagaa aattcagcct ttctggattg aatcagaagc
     2761 aaaaaaaatt cccggaacaa attatatcaa cgggggactt ttcagagagt ttgcagtcag
     2821 agacggaaat ctgatcacag gacagcagca gttcagcggt gcggccgcag caagacttgt
     2881 aatagaagca ctgggagtat aaggat
//
LOCUS       DAOQGJ010000282         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20795_length_2906_cov_2.605752, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000282 DAOQGJ010000000
VERSION     DAOQGJ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20795_length_2906_cov_2.605752"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PK683_05715"
     CDS             complement(<1..822)
                     /locus_tag="PK683_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH07979.1"
                     /translation="MKKYKGAVYIGRFQPPHKAHIQTVEKALELAEKAVIIAGDYKSA
                     RSVKNPFTFDERKEMLLGCLKKEDSERVIFCPVRNFPNDDSLWTVSIKKEVSAHIKEN
                     LCITGHVKDSSSYYLKLFPEWEFESIDLISDLNATDIRNIFFQKKDFFNDKKITESLH
                     SSVVSFLKEFALTESFSILQEDWFHLAEYKASWKAAPYPPVFVTADCAVLKSGHILLV
                     KRKSSPGKGLFAVPGGFLDQNETLLDCAIRELKEETEIDLSPSELKDRVSVSKVFD"
     gene            complement(847..1230)
                     /locus_tag="PK683_05720"
     CDS             complement(847..1230)
                     /locus_tag="PK683_05720"
                     /inference="COORDINATES: protein motif:HMM:NF025471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HNH07980.1"
                     /translation="MKQPKAWVSLVVSSKELSPKQITEFLKLQPDFTNPVEAKSKKGN
                     FWQLNSSLPDEENLENHLWDLLKKIAPVRDELKDLHIKAEIKFYASVEFADTETNGLQ
                     LGPRLLSLIGSLGINLEIHPWKGKM"
     gene            1452..1658
                     /locus_tag="PK683_05725"
     CDS             1452..1658
                     /locus_tag="PK683_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020762606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07981.1"
                     /translation="MWLKLGDTEAINLDFVSTIKKNLNNPTIEIIYHDLNNIKSLPFK
                     TADERDKAFKAIMENLARMKLFFE"
     gene            1651..2439
                     /locus_tag="PK683_05730"
     CDS             1651..2439
                     /locus_tag="PK683_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07982.1"
                     /translation="MNRMKLEESIDMDWEKIRLHLRKVFSVSPFHIYFYGSRERGDFK
                     EKSDYNFYLIASAEDQIRSNFIQEITKALESVGRKPVNLIAGDRESFIQRMEIFEPTA
                     VHLLELGRMIYGEREFLPVINEWKSVRKGTIDHLQILKYLENRTKFYKTLKPRQSRDD
                     ISRIEKILALTVQTWIIGCTDDLTVTELAFMDIPERLIRMVRSLYASQVPKDILLLAT
                     IYEEIHELKRNMRSLSGTEKSDLDKIKESILQIKDISFEIGNFA"
     gene            complement(2455..>2906)
                     /locus_tag="PK683_05735"
     CDS             complement(2455..>2906)
                     /locus_tag="PK683_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HNH07983.1"
                     /translation="VLAKTEQDSAKYSSYSYDDLFFLVFLNHIEPRLTEGPFFVHSYP
                     RELASLAKTEGRIARRFEIYWKNVEIGNAFYELNEMQEQRKRFEEEQEQRRALGKEVF
                     PLDESFLESLKNLPECSGISIGLDRLFMICMNHDSLKLLSPYANQLG"
BASE COUNT          850 a          595 c          591 g          870 t
ORIGIN      
        1 gtcaaaaacc tttgatactg atactcggtc ctttaattct gaaggactca aatctatttc
       61 ggtttcttcc ttcaattcac ggatggcaca gtccagcaga gtttcattct gatccagaaa
      121 tcctcccggc actgcaaaca ggccttttcc tggactcgat tttcttttta ccagaaggat
      181 atgaccgctt ttcagtacag cacagtcggc agtcacaaat acaggggggt agggtgcagc
      241 cttccaactc gccttatact ctgcaagatg gaaccagtct tcctgcagaa tggagaaact
      301 ttcagtcagc gcgaactctt tcagaaatga gacgacagag gaatgaaggg actctgtgat
      361 ttttttatca ttaaaaaaat cttttttttg aaaaaaaata ttccttatgt ccgttgcatt
      421 cagatcgctg atcaggtcta ttgattcaaa ttcccattcc ggaaacagtt tcagataata
      481 gcttgaggag tcttttacgt ggccggtaat gcagagattt tctttgatat gtgcacttac
      541 ttcttttttt atactgactg tccagaggct gtcgtcattc gggaaatttc ttaccggaca
      601 gaatatcacc ctttcactgt cttctttttt aagacatccg agaagcatct cttttctttc
      661 atcaaatgta aacgggtttt ttactgatcg tgcggacttg tagtcgcctg ctatgatgac
      721 tgctttctct gcaagttcaa gagctttttc tacagtctga atatgagcct tgtgaggggg
      781 ctgaaatctt cctatataaa ctgcaccttt gtattttttc attatattcc tttcccgaat
      841 caggctttac atttttccct tccaggggtg aatttccaag ttgataccca ggctgcctat
      901 cagtgaaaga agcctcggac cgagctgcag tccgtttgtt tctgtatccg caaattccac
      961 agaagcgtaa aacttaattt ctgccttgat gtgcaggtcc ttgagttcgt ctctgaccgg
     1021 tgcaattttt tttaacagat cccatagatg attttccaga ttttcttcgt cgggaagaga
     1081 ggaattcagc tgccagaaat tgcctttttt gctttttgct tctactggat ttgtaaaatc
     1141 gggctggagt ttcaaaaatt cagtaatctg tttagggctc agttccttgg aagaaactac
     1201 aagagacacc caggctttcg gctgtttcat gcgaacagtc tccaagttta cagtcatttg
     1261 taaagcatat tccccaaaaa taggaactgc ggggagatgc attctgtaat tcagggttcc
     1321 gaattttcag acagatatat atttcaacta caagaaaata gtcatctaaa tcagtgtttc
     1381 atacgaaaat agtaaggaaa gctcattctg cagctgtccg cagcagtgac atccaaagga
     1441 gttcagaaaa tatgtggctg aaattaggtg atacagaggc aatcaatctg gattttgtat
     1501 cgactattaa gaagaatctg aacaatccta cgatcgaaat tatctaccat gacttgaaca
     1561 atataaagtc ccttccattt aagactgctg acgagagaga caaggcattc aaggctatta
     1621 tggaaaatct ggccagaatg aaactgtttt ttgaatagaa tgaaactgga agagagcata
     1681 gatatggact gggaaaaaat ccggctccac cttaggaaag ttttttccgt ttctccgttt
     1741 catatatatt tttacggatc gagagaacga ggggatttca aggaaaaatc cgattacaat
     1801 ttctatctga ttgcctcagc agaggatcag attcggtcaa attttattca ggaaattaca
     1861 aaagcactgg aatctgtcgg aagaaagcct gtgaatctga ttgcaggcga cagagaatca
     1921 ttcattcaga gaatggagat ttttgaacct acagcagttc atcttcttga actgggacgg
     1981 atgatttacg gagaaagaga atttcttcct gtaatcaatg aatggaaatc tgtccggaaa
     2041 ggaacaatag accatcttca gatactgaaa tatctcgaaa acagaaccaa gttttacaaa
     2101 acgctgaaac cgagacagtc tcgagacgac atcagcagga tagagaaaat attggcgctg
     2161 actgtccaga catggataat cggctgcaca gatgatttaa cagtgactga acttgcattt
     2221 atggatattc cagaaagact catccggatg gtaaggtcgc tgtatgcaag tcaggtgcct
     2281 aaggacattc ttcttcttgc gacaatctat gaagaaattc atgaactgaa aagaaatatg
     2341 agatcgcttt caggaacaga aaaaagcgat ttggacaaaa tcaaggaatc cattctgcag
     2401 atcaaggata tcagtttcga aataggaaac ttcgcctgac ggccgcctga cggcttatcc
     2461 cagctgattt gcataaggac tcagaagttt cagtgaatcg tggttcatac atatcataaa
     2521 cagcctgtcc agaccgatgg agattcctga acattccgga agatttttta atgattccag
     2581 aaaactttca tcgagcggga atacttcctt ccccagagct cttctctgtt cctgttcttc
     2641 ctcaaatctt ttcctctgtt cctgcatctc attcagttca taaaatgcat tgcctatttc
     2701 aacattcttc cagtatattt caaatcgtct cgcaatccgt ccttcagtct ttgccaggga
     2761 tgccagttct cttggataac tgtgaacaaa gaagggacct tctgtcagtc tgggttcaat
     2821 atgattcaga aatacaagaa agaaaagatc atcataggaa taagaggagt atttcgcact
     2881 gtcctgttca gttttagcca gaactg
//
LOCUS       DAOQGJ010000283         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20796_length_2906_cov_2.560505, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000283 DAOQGJ010000000
VERSION     DAOQGJ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20796_length_2906_cov_2.560505"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..913
                     /locus_tag="PK683_05740"
     CDS             83..913
                     /locus_tag="PK683_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNH07984.1"
                     /translation="MEQLRKFLAEKKKHLSVIIIGLQGLGGLALGSYFIRPAIVGTAA
                     VAVTNPIQACDNNSYSQISCKCESGTTNSTVKAAAVVIAASNASKSTSTGTSTSTNKK
                     LFLTAAKYDGNLGGVSGADSKCNSDSAKPSGTSTYKAFITDVTNRVACTTNNCSGGVS
                     ENKDWVLKASTSYYKSDGTTLVFTTNASGIFANGAIKAAVGNSTDETWTGIYSNGADG
                     GWTNAYVAHCSNWSSNSSSGTGGLRGFPGVIPTSFVGDWFGKNVATCDTTRYILCVEQ
                     "
     gene            1041..2078
                     /locus_tag="PK683_05745"
     CDS             1041..2078
                     /locus_tag="PK683_05745"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH07985.1"
                     /translation="MYPKIIIWKYMFNGEGLFTFLAVVGAFLHIFLFVLPKSGYSRTA
                     FINGFLFSIVLGVIGTIIMNYLIWDFDLFSENPSILLGALEKNQGSTILGGILGLSAG
                     VWIFCRFHKINALHALSSLAAVAPILWMGLGRTACFLNGDAFGKATSSVLGVTFSEKS
                     SSWMPTWVGYSELYKNDEKPLEKIEIIFTKLHGVGLSEMPIPDSLDHLKKEGFKNLAD
                     LKVFYPEGTSIDREKLAAKGLVPFPVVYPKVHPTQIYDMISLTIWFFILNWLSKKKST
                     EQRMIFLFFAAYGLNRFWIEFFRDDISPGVLGLTGAQLVCLGFISFGLGAFYYYTKKW
                     EKTGLPEVIFI"
     gene            2085..>2906
                     /locus_tag="PK683_05750"
     CDS             2085..>2906
                     /locus_tag="PK683_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH07986.1"
                     /translation="MKDTAFITGASGFIGSHLADFLLQKGWKVICLVRPSSNLQWLKD
                     KPVTVLSADISEREKISPLVSESTHIFHLAGVLFGKDREEYIQGNLEYTKRLLNLIKT
                     AKPNLKRFLYVGTLASAGPSENGKLPDESDELKPITWYGESKALAENFLKENIGEIPL
                     TVIRPPVVYGPRDYAMFELFKASRLGLNLIPGNKNKYISIIYIKDLIQGIYDACISET
                     TMGRTYFLANEEYCPQYHLGEIIIRKMNRSPVNVVIPWIIMRAVSNLLDFFGKLFG"
BASE COUNT          872 a          602 c          632 g          800 t
ORIGIN      
        1 agtcatctaa tgcagaacct tactaccctg caataaagac ttgtctgtct atacttcatt
       61 ttgacacaaa caggagagac gaatggaaca attgaggaaa tttttagcag agaagaaaaa
      121 gcatctgagc gtcatcatca tcggactgca gggattggga gggcttgcat taggaagtta
      181 cttcatcaga ccggcaattg taggcactgc agccgtggct gttacgaatc cgatacaagc
      241 ttgtgacaat aatagttatt cacaaatttc ctgtaaatgt gaaagcggga ctaccaactc
      301 caccgtgaag gcagcagcgg ttgttatagc tgcttccaat gcatccaaga gcacctcgac
      361 tggcacatcc acaagcacca acaaaaagct gtttctcact gcagcaaagt atgacggaaa
      421 tttaggcggg gtttccggtg cagattcgaa atgcaactcc gattctgcaa aaccttccgg
      481 aacaagcaca tacaaagcat ttatcacgga cgtgaccaac cgtgttgcat gcactacaaa
      541 caactgctcc ggcggagtga gcgaaaacaa agactgggta ctgaaagcca gtacaagtta
      601 ttacaagtct gacggaacaa ctcttgtatt cactacaaat gcttccggta tatttgcaaa
      661 cggtgcaatc aaagccgcag tgggcaattc cacagatgaa acttggaccg gaatctacag
      721 caacggggca gacgggggct ggacaaacgc ttatgtagct cattgttcaa actggtcttc
      781 caattcctct tccggaaccg gaggactgag aggatttccc ggagtcatcc ctacttcttt
      841 tgtaggcgac tggtttggga aaaacgttgc cacctgcgat acgaccagat acattctctg
      901 tgtagaacag taggagtaaa ataagtcgtc ttcattcagg acaggcttat gcttggatga
      961 agacactgct gattcgcctg aatacggcca atgtaaaaca tcaaataaat ctgaattaaa
     1021 taatatataa ggaaaataca atgtatccta aaatcattat atggaaatat atgttcaatg
     1081 gtgaaggact ctttactttc cttgcagtag tcggtgcctt tcttcatata tttctgtttg
     1141 tactgccgaa atcgggttat tccagaactg cattcatcaa cggatttcta ttttctattg
     1201 tcttaggtgt catcggtacg ataataatga attatcttat ctgggacttc gatcttttca
     1261 gcgaaaaccc atctatccta ttaggtgctc tggagaaaaa tcagggatcc accatccttg
     1321 gcggaatact cggtctgtct gcaggggtat ggatattctg cagatttcat aaaatcaatg
     1381 cacttcatgc tctttcttcc ttggctgcag tggctcccat tctctggatg ggtcttggaa
     1441 gaactgcctg ctttttgaac ggtgatgcat ttggcaaagc gaccagctca gttttaggag
     1501 taacattttc agaaaagagt tcttcctgga tgcccacatg ggttggttat tccgagctgt
     1561 acaaaaatga tgaaaaacca ttggaaaaaa tagaaattat atttacaaaa ctccacggag
     1621 tcggactttc tgagatgccg attccggaca gtctggatca tctgaaaaaa gaaggattca
     1681 agaatctggc cgatctgaaa gtattttatc ctgaaggaac ttccattgac agagaaaagc
     1741 tggctgcaaa aggcctggtt cctttccctg tagtgtatcc taaagtgcat ccgactcaga
     1801 tctatgatat gatttcactt acaatctggt tctttatact aaactggctt tccaaaaaaa
     1861 aatcgacaga acagagaatg atttttctgt tctttgctgc atacggtttg aacagattct
     1921 ggatagaatt ttttcgggac gatataagcc ctggagttct gggattgaca ggtgcgcagc
     1981 tggtttgcct gggcttcatc agtttcgggc tgggagcatt ttattattat acaaaaaaat
     2041 gggaaaaaac aggactccct gaagtcattt ttatataaat tatcatgaaa gatactgctt
     2101 ttatcacagg cgcttccgga ttcataggat cacatttggc agattttctt cttcagaaag
     2161 gctggaaagt gatatgtttg gtcagaccca gttccaatct gcagtggctg aaagacaagc
     2221 cagttaccgt cttaagtgca gatatcagtg agagggaaaa aatttctcct cttgtctccg
     2281 aaagcactca tatttttcac ctggcaggtg ttttatttgg aaaagacagg gaggaataca
     2341 ttcagggaaa tctggaatac acaaaaagac ttttgaacct gataaagacc gcgaagccga
     2401 atctgaaacg atttctttac gtcggcacac ttgcatcggc cggtccatcg gaaaacggaa
     2461 aacttccaga cgagtctgat gaactgaaac cgattacctg gtacggagaa agcaaggccc
     2521 ttgctgaaaa ttttctgaag gaaaatattg gtgagattcc actgactgta atcagaccgc
     2581 cagtcgtcta cggcccccgt gattatgcaa tgtttgaact tttcaaggcc tccagactgg
     2641 gactgaatct gattccagga aataaaaata aatatatcag cattatatat atcaaggact
     2701 taattcaggg aatatatgat gcctgcatct ctgaaaccac aatgggcaga acatattttc
     2761 ttgcaaatga ggaatactgt cctcagtatc atcttggaga aatcattatc cgtaaaatga
     2821 acaggagccc tgtaaatgtc gtcataccct ggattattat gcgagcagtc tcaaatctgc
     2881 tcgatttttt cggaaaactc ttcggg
//
LOCUS       DAOQGJ010000284         2899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20885_length_2899_cov_2.891702, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000284 DAOQGJ010000000
VERSION     DAOQGJ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20885_length_2899_cov_2.891702"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK683_05755"
     CDS             complement(<1..580)
                     /locus_tag="PK683_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNH07987.1"
                     /translation="MIINHNVSAINSHRVLKFNNEVLGKDMEKLSSGMRINRAGDDAS
                     GLAVSEKMRSQVNGLRRAEQNAEDGISFIQTTEGYLQETQDIVQRIRVLAVQAANGIY
                     TEEDRQQIQVEVSQLIDEIDRIASQAEFNKMKMLTGSFARPNPTASMWFHIGANMHQR
                     ERVFIETMTTAGLGLRNPTVLTFISLSTMSKAN"
     gene            900..2042
                     /locus_tag="PK683_05760"
     CDS             900..2042
                     /locus_tag="PK683_05760"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH07988.1"
                     /translation="MGSRIAGKILEKSRKSILLLGPRQVGKSTLLKGLNPDIIINLAS
                     ESEYLRYLSDPGLLVSVLKGGFYKTVLIDEIQRIPSLLNTIQMIIDDWDRAPKFYLSG
                     SSARKLKKGNANLLPGRIHQYSLSGFSGSDLEYRLDLEKAIRFGFLPEPYLEGEENVC
                     RKILRDYAAGYLKEEIQAEALSRNIQGFARFLQTVGGVSGSILDFSKIASKAKVSRSS
                     AVRFLEILEDTLIAERIENFPETDADTVRHPKLYFFDIGVLNGLLGNFEPSADRIGKS
                     FEHLVYSQIRNSAFAKDEQIELFYFRTRNGLEVDFILKLQGKVYAIEANAGDVHASDL
                     RSLIRFREYCPKLHQCIAVSLKEDKIRNTDNIMICGVSEMLQLLGL"
     gene            complement(2096..2662)
                     /locus_tag="PK683_05765"
     CDS             complement(2096..2662)
                     /locus_tag="PK683_05765"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH07989.1"
                     /translation="MSTLLEKLTVSEYLEKERNSNLRAEYIRGEERKLTGASFIHNKM
                     VINLIRGITSKLNSRKWEILSSDMKVWIPAKETFYYPDVLILPNPPLFHDEFKDIVTN
                     PVCIFEVLSESTRNFDKGEKFDTYRSIPQFQEYFLVEQTKKLVTQYIINSKGNWELIE
                     YKDLQSVFALPCLNIELALSEVYDGIEI"
BASE COUNT          818 a          544 c          621 g          916 t
ORIGIN      
        1 cattagcttt tgacatagta gaaagagaga tgaaagtaag aactgttggg tttctcagtc
       61 cgagtccagc agtagtcata gtttcgatga atactctttc tctttggtgc atgtttgctc
      121 caatgtggaa ccacatagaa gcagttgggt tcggtcttgc aaaagaccca gtcagcattt
      181 tcattttatt gaattctgcc tgagaagcaa ttctgtcgat ttcatcaatc agctgagaaa
      241 cttcaacttg gatctgctgt ctgtcttctt cagtgtaaat accgttagca gcttgaacag
      301 ccagtacacg gattctctga acgatgtctt gagtttcttg cagataacct tcagtagtct
      361 ggatgaaaga aattccatct tctgcgtttt gctctgcacg tctgagaccg ttaacttgag
      421 atctcatttt ttcagaaact gcaagtccgg aagcgtcatc acctgcacgg ttgattctca
      481 tgcctgaaga cagtttttcc atgtcttttc cgagaacttc gttgttgaac ttgagtactc
      541 tgtgtgagtt gattgctgat acgttgtggt tgataatcat attcggtttc ctccatgaaa
      601 tccgtctttc ttgcgaaaga agtgtgaaag tccatccttg gaccctcata ctgtatatcg
      661 gttttttcgt ccgaaggttg aactatgtaa agtttttttt gggaaatttc ggaaaaaaac
      721 ttacatttga ttttctgcaa aatgcttttc atcagcaaaa ccctatttcg agcggggaaa
      781 gttctaaaca gagttggaca gatttattca gcttatgcac aacctcaaac ttgtaaggaa
      841 ccatcagata caagtaaaaa tgatttgcat ctgagcagat tacccgtaaa tttgaccgta
      901 tgggtagcag aattgcgggt aagatattag aaaagagcag gaaatcgatt cttctgcttg
      961 gacctcggca ggttggaaag tcgactcttt taaaaggtct caatccagac atcataatca
     1021 atctggcttc cgagtcagag tatctccggt atctctctga tccggggctt cttgtttccg
     1081 ttttaaaagg gggtttttat aagacagtat tgattgatga gattcagaga attcccagtt
     1141 tgctgaatac aattcagatg attattgatg attgggacag agcgcccaag ttttatctgt
     1201 ctggttcgag tgcccgtaaa cttaaaaagg gaaatgcaaa ccttctcccc ggaaggattc
     1261 atcagtattc cctttccggt ttttccggaa gcgatctgga atatcggctg gatctggaaa
     1321 aggcaatccg atttggattt ctgcccgaac cgtatcttga gggagaggaa aatgtgtgca
     1381 gaaaaatctt aagggattat gcagccggtt atctgaaaga ggagatacag gcagaagcac
     1441 tgtcacgaaa cattcaaggg tttgccagat ttctgcagac agtcggtgga gtgtcgggaa
     1501 gtatattaga tttttctaaa atagcatcca aggcaaaagt atcccgttct tctgcagtac
     1561 ggtttctgga gattttggag gatacactga ttgctgaaag aatagaaaat tttccagaaa
     1621 cagatgctga tactgtacgg catccgaaac tctatttttt tgatatcggt gtattaaatg
     1681 ggcttctggg aaattttgaa ccatctgcgg acagaatcgg aaaaagtttt gaacatctgg
     1741 tgtacagtca gataagaaat tctgcatttg cgaaggatga acagattgaa ctgttttact
     1801 tcagaacaag aaacggattg gaagtagatt ttatactgaa attacaaggg aaagtttatg
     1861 caatcgaagc aaatgcaggt gatgttcacg caagtgatct gcgttctctg attaggtttc
     1921 gggaatactg ccctaagctg catcagtgca ttgccgtcag tctgaaagaa gataaaatac
     1981 gaaatacaga taatataatg atctgcggag tcagtgaaat gctgcagctg ctgggattat
     2041 agcatccttc tgcagtttct gatccatact tgccaatact ctgttttgac tgaatctaaa
     2101 tttctattcc gtcatagact tctgacagag caagttctat gttcaggcag ggaagggcga
     2161 agacagactg taagtcttta tattctataa gctcccaatt tccttttgaa tttatgatgt
     2221 actgagtgac gagtttttta gtctgctcca caagaaagta ttcctgaaac tgaggaatgc
     2281 ttctgtaagt gtcgaacttt tctcccttgt caaaatttct ggtagattcc gagagcactt
     2341 caaaaatgca gactggattg gttacaatat ccttgaattc atcgtggaac agcggagggt
     2401 tcgggagaat cagtacatcc gggtaataaa atgtctcctt tgccggaatc catactttca
     2461 tatcactcga gaggatttcc catttacggc tgttcagttt tgaagttatt cctctgatca
     2521 ggtttatgac cattttgtta tgtataaaac ttgcaccagt caattttctt tcctctcctc
     2581 gaatatattc tgctctgagg ttcgaatttc tttctttctc aagatattcg gaaacagtaa
     2641 gtttttccag taaagtgctc atcttaacct ctcaaaatct tggtcgctca actctatttt
     2701 taagcaagtg tttttaattt tcaagtaaaa attgacttga atattgcagg ctctttttgc
     2761 atcagttact gtactggacc taccacagcg gaaaaagaaa aaaagcctgt cgaaggctgt
     2821 tttatttgcc gaatatagaa tccaggattt catcgtatct ttcatctgaa attccgaaca
     2881 gattcagaag atagtcaat
//
LOCUS       DAOQGJ010000285         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_20891_length_2898_cov_3.579669, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000285 DAOQGJ010000000
VERSION     DAOQGJ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20891_length_2898_cov_3.579669"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PK683_05770"
     CDS             complement(<1..1138)
                     /locus_tag="PK683_05770"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNH07990.1"
                     /translation="MDIKKYKEGLPYRFFFPFSLIGAVSAVSLWILAYAAERGWISPL
                     KNPYPVQHHILLISSLFLLPALKGFLFTAIPRFTGTDFLGFYSIGLLLSLQILISVLA
                     LFFENAILFYFLLNLDFLLLLGFVVIRFSKAKGKLSEYLYFIPAGLFLGTVGSVFLSI
                     SRIREDISLFHYGKELIVYGMIPCAVFGAGIRIVNMILNRENPEKRSVWMQKAESIQN
                     GRIFSLIFVFFSLSESAVFYLFSLEYSVFFRTLRFLICIFLFIRYFQIFDLSMYKGRL
                     SFLILISLYSFLLGLAGHAWGGAYSVHMVHLYLISGLSLFIAGIMTRVVFSHEGLNLD
                     LEKNSSVFLWVAVLFLLAALTRASAVFMPSLMISHFAYASFLFIL"
     gene            1247..1876
                     /locus_tag="PK683_05775"
     CDS             1247..1876
                     /locus_tag="PK683_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH07991.1"
                     /translation="MSSNFWSTIPSSIRIELERISQNRKFQKGEIVFSENEAFRGFAV
                     IRSGKFKIYNLNLSGKEAILRVLEEGEMAAAPLIFSEHPVYPATLECLDAGSVYFFEA
                     VQFRQFLKNHSEFQMQFTGLIMQFMHYLKNKTSSHMLLNLKERMLEYLKEQGAELNFI
                     SLPINKNQLALLLDATPESISRMFKSLEEEGILEVKGKKYRLKQSEMTA"
     gene            complement(1881..2564)
                     /locus_tag="PK683_05780"
     CDS             complement(1881..2564)
                     /locus_tag="PK683_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNH07992.1"
                     /translation="MISGAERGSSFDPRSIALRSPERLQDWEILAILFGTGTRGKDVA
                     SLSKELLAEFSSLSSLIFAPGSVLQKKTGLGRAKTALLAAVRELMYRVNTSDLLKEPT
                     VDFRKLQDILYQRTVSEVRESFYLISLGSSGCLLEIDLLAKGGLKEVGIRFRDLLKTV
                     LDTGAHHTILCHNHPGDTPEPSEEDWTLFYSVKELFLRLEVDLLDQWIMGRNGIYSCD
                     KKQMIRKMK"
     gene            complement(2561..2782)
                     /locus_tag="PK683_05785"
     CDS             complement(2561..2782)
                     /locus_tag="PK683_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07993.1"
                     /translation="MVIKSLEYPVNYRRRTAGLAGTGSGKALGKNSAKSFEEYLMEAF
                     QGELVQNGNLFSAHLSAQTKTNLSKIRSA"
BASE COUNT          999 a          591 c          566 g          742 t
ORIGIN      
        1 cgagaataaa cagaaaagag gcatatgcga aatgagaaat cattaatgaa ggcataaata
       61 ctgcagatgc tctggtgaga gctgcaagaa ggaaaagaac cgccacccag agaaaaacgg
      121 acgaattttt ctccagatcc agattcagtc cttcatgaga aaagaccact cttgtcatta
      181 ttcctgcaat aaaaagagaa agacctgaaa tcagataaag atgaaccata tgaacggaat
      241 aagcgcctcc ccaggcatga cccgcaaggc ccaacagaaa ggaatacagt gaaatcagta
      301 tcagaaaaga cagtctgcct ttgtacatgg aaagatcaaa aatctgaaaa tacctgataa
      361 aaagaaaaat acagatcaaa aaacgcagtg ttctgaaaaa tacagaatat tccagagaaa
      421 acaggtaaaa aacagcactt tcagataatg aaaaaaacac aaaaattaag gaaaaaatcc
      481 tgccattctg aatagattca gccttctgca tccagacaga tcttttttcc ggattttctc
      541 tgttcagaat catattgaca attctgattc ctgctccgaa aactgcacag ggtatcatac
      601 cataaacaat caactctttg ccgtaatgaa aaagtgaaat gtcttctctg attctgctga
      661 tgcttaagaa aacggagccg acagttccca aaaatagtcc tgccggaata aaatacagat
      721 attcggagag ttttcccttg gctttgctga atcggatgac cacaaaccct aaaaggagca
      781 aaaaatccaa attgagcaaa aaataaaaga gaatcgcatt ttcaaagaaa agagcaagca
      841 ctgaaatcag aatctgcagg gaaagaagaa gaccaatcga atagaaccct aagaaatctg
      901 ttccagtaaa tcttggaatt gcagtgaaaa gaaacccctt cagggcaggc aggagaaaaa
      961 gcgatgaaat cagaagaata tggtgctgca caggataagg attttttaag ggagatatcc
     1021 atcccctttc agccgcatag gcaagtatcc atagagaaac tgcactgact gccccgatca
     1081 gagaaaaagg aaaaaagaac cggtaaggca gaccttcttt atattttttt atatccataa
     1141 tgagaaaaac ccctttgaaa aataaaacag ttaagctggt acaaattact tgtagctttt
     1201 cgaatcaaaa atagcattga tttaaatcaa cgctttcgga gaatttatga gttcaaattt
     1261 ctggagtact atcccatctt caatccgaat tgaattggaa aggatttctc agaacaggaa
     1321 attccagaaa ggggaaattg ttttttcaga gaacgaagct ttcagaggat ttgcagtcat
     1381 ccgctcagga aaattcaaaa tatacaattt gaatttaagc ggaaaagaag caattctaag
     1441 agtgctcgaa gaaggagaaa tggcagctgc acctcttatc ttctcagaac atcctgtcta
     1501 tccggctaca ctggaatgcc ttgatgcagg ctctgtatat ttttttgaag ccgttcagtt
     1561 cagacagttc ctgaaaaatc attccgaatt tcagatgcag ttcactgggc tcattatgca
     1621 gtttatgcat tatctgaaaa acaaaacgtc ttctcatatg cttctgaatc tgaaagaaag
     1681 gatgctggaa tatttgaaag aacagggagc agaactcaat tttatctcac ttcccatcaa
     1741 taagaaccag cttgcacttc ttctggatgc aacgccggag tcgatcagca gaatgttcaa
     1801 gtctctcgaa gaagagggaa ttttggaagt taaaggaaaa aaatacaggc tgaaacagtc
     1861 ggaaatgact gcataacaga ttatttcatt tttctgatca tttgtttctt atcgcaggaa
     1921 taaattccat ttcgtcccat aatccattgg tccaaaagat cgacttcaag tctgagaaat
     1981 aattccttta ctgaataaaa aagggtccaa tcctcctcgc tgggttcagg agtatcgccc
     2041 ggatgattat gacacaaaat tgtatgatgt gcacctgtat ccagaacagt cttgagcaga
     2101 tccctgaaac ggatgcctac ttccttcaga cctcccttgg caagaagatc tatttccaaa
     2161 agacatcctg aacttccaag agagatgagg tagaaacttt ctctgacttc tgaaacggtt
     2221 ctctggtaaa gtatgtcctg aagttttctg aagtccacag taggttcttt caaaagatcc
     2281 gaagtattga ctctgtacat aagttctctg acagcagcaa gaagagctgt ctttgcccgt
     2341 ccaagtcctg tctttttctg taagacagaa cctggcgcaa aaatcagact cgacagactg
     2401 gaaaactcgg ccagaagttc ttttgacagg gatgcaacat cctttcctct tgttcctgtt
     2461 ccgaacagga tagccagtat ctcccagtcc tgaagacgtt caggagaacg aagggctata
     2521 gatctaggat cgaatgatga tcctctttct gcaccggata tcaagcagaa cgaatcttgg
     2581 aaagattcgt ttttgtctgt gctgacagat gcgcagaaaa gaggttcccg ttttgtacga
     2641 gttctccctg aaaagcttcc ataagatact cttcaaaact cttcgcagag tttttaccga
     2701 gagctttccc agaaccggtt ccggcgagac cagcagttct cctgcgatag tttacaggat
     2761 attccaagga tttgataacc atagtgaacc tccatgctct ttcttagacg acataattcc
     2821 aaaaaagcgt tctttttttt gaaaaaaaat ccgaagcaga tatttttctc tgctgacaga
     2881 accaagtcaa aaactact
//
LOCUS       DAOQGJ010000286         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21045_length_2885_cov_3.454770, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000286 DAOQGJ010000000
VERSION     DAOQGJ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21045_length_2885_cov_3.454770"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PK683_05790"
     CDS             complement(<1..758)
                     /locus_tag="PK683_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07994.1"
                     /translation="MKLGIITNTLSTRNEGLTTHDLAFGALIKGHEVYFIPVASLSSD
                     GRNIFSNAKRLTKEGVLKREDLTHRLKDFPDFRLCLGDLNVLLLRHKFDSGSIPETYH
                     KCAREYAFHLQEKGVFVANNPLYLPFVSSKLSTIGISEEILPKHQLVSSNFEELYSYC
                     KDELKYEGVIKPLGGKGGEDIYFTEKKNLRNNLRALLKKGAVMVQNFIPNDGDKRILL
                     LNGNPVAWYKRVALEGEFINNIHAGGRPVKFDLT"
     gene            978..1781
                     /locus_tag="PK683_05795"
     CDS             978..1781
                     /locus_tag="PK683_05795"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNH07995.1"
                     /translation="MKNRQVFKGKVNSQNEFELVMTTPEGKKLNFSKKTILKVLYKDL
                     NENEVKKVIQEEEIKQGITAKDTPGKKDVPGKKDPEPDTSGPKSPEVRSKWMIAWRSA
                     VVPGWGQLKAKRYWTASAAFLVPLGIAGYLGSTYKNYQDAEKQYQSKAAFTALATGAA
                     TSSSSISTTTALFLNIAMNNSAFSPYEAAKNTFNNTAPLLGVAYALQLAHAYYVGKQW
                     EVEGTSNAVTIDGKSINEGWSFNASMQRNIFSGQLTPNFEMSYAIHFKD"
     gene            complement(1778..>2885)
                     /locus_tag="PK683_05800"
     CDS             complement(1778..>2885)
                     /locus_tag="PK683_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNH07996.1"
                     /translation="SDSAVAFIEFSRTPYLEPSTRAYFPVFILILCLMSGMAAFSGNL
                     LSIAKIRLRIPVRNYSYPMQTGLNLMFIFLLGVLMFSLLLKGDRDFALLSLSAFFAFL
                     YALIIMFPVIGTDMPVVIAGLNSVSGFSAFLFGLYSENQIMILTGTVTCAAGFMLARK
                     VSFSMNRNLFSVFLGISPKQESEQHTGSFEAVIINEINVSDAAIILAFAKRVVIVPGY
                     GFAASQANHVCKELVTLLEEREIQVKFAIHPLAGRMPGHMNVLLSEAEIPYDRIVDTD
                     HSNEEIRIADVCLVIGGNDIVNPAVLKKKKSNIAGLPLFRPELAKNAIILKRGKGKGF
                     SGIRNEIFDLENVHILRGEAKNIIVRLANEIREI"
BASE COUNT          904 a          618 c          571 g          792 t
ORIGIN      
        1 tctgtcagat caaatttcac aggtctgccg ccggcatgaa tattgttgat aaactctcct
       61 tcaagtgcaa ctctcttgta ccatgctacc ggatttccat tcagaaggag aatccgttta
      121 tctccgtcat tcggaataaa gttctgcacc ataacagcac ctttcttcaa aagtgcgcgg
      181 agattgtttc tcagattctt cttttccgtg aaataaatat cctctcctcc ctttcctccc
      241 agcggtttga tcactccctc atacttgagt tcatccttac agtaactgta aagttcttcg
      301 aaattagacg aaacaagctg atgtttcggc aggatttctt ctgaaattcc gattgtagaa
      361 agtttggatg aaacgaatgg aaggtacagt ggattgtttg ctacaaaaac gcctttctcc
      421 tgaagatgaa aagcatactc tctggcacat ttatggtacg tttccggaat tgaacctgaa
      481 tcaaatttat gcctaagaag aagaacattc aaatctccca aacagagtct gaaatcagga
      541 aaatccttca gccgatgagt cagatcctct cttttaagta caccttcttt cgtgagtcgc
      601 tttgcatttg aaaaaatatt ccgtccgtcg gaagagagac tggcaaccgg aataaagtac
      661 acttcatgac ctttgatcag tgcaccgaag gcaagatcat gagttgtcag cccttcattt
      721 cttgtagaca gtgtgttggt gataataccc agcttcatat cggacttgct atattctcga
      781 atttgaacag tgaaaaaaag aggaaaaatt caaatatcga gctggatttt agaaaaatcc
      841 cctattttta attctttgca gaatgattta gtgaaaaata ctgtctaagg aaggatacta
      901 tgcaaagtaa gctattttat caatctttaa ttgcgttttt tctattttcg acagggattc
      961 tggctgaaac tgttatcttg aaaaacagac aggtttttaa gggaaaagtc aattctcaaa
     1021 atgaatttga acttgttatg accaccccag aaggcaagaa gcttaatttt tccaaaaaaa
     1081 ctatactgaa ggttctgtat aaggacctga atgaaaacga agtaaagaaa gtcattcagg
     1141 aagaggaaat caaacaggga atcactgcaa aggatacccc tggcaaaaaa gacgttcctg
     1201 gcaagaaaga tcctgaacct gacacgagcg gccccaaaag tcctgaagtc agaagcaaat
     1261 ggatgatcgc ctggagatcg gctgttgttc ccggatgggg acagctgaaa gccaagagat
     1321 actggaccgc atctgctgca tttttagtcc cactggggat tgccggttat ctgggatcta
     1381 catataaaaa ttaccaagat gcagaaaaac aatatcaaag caaagcagca tttactgcac
     1441 ttgcaaccgg tgcagcaaca tcatccagtt ctatttccac aaccactgca ttgtttttga
     1501 atattgcgat gaataattct gcattttctc catacgaagc cgcaaaaaat acattcaaca
     1561 acactgcgcc tcttttgggt gtggcttatg cacttcaact ggctcatgcc tactatgtag
     1621 gaaaacagtg ggaagtggaa ggaacatcca atgcagtcac gattgatgga aagtccatta
     1681 atgagggctg gagtttcaat gcttcaatgc agagaaacat attcagcggt cagctcactc
     1741 caaattttga aatgtcctac gcaattcact tcaaagatta aatctctcga atctcgttcg
     1801 caagtcttac gataatgttt tttgcctcac cccgaagaat gtgaacattt tcgagatcaa
     1861 agatctcatt tctgattccg gaaaatccct ttccttttcc tctctttaag atgatggcat
     1921 tttttgccag ttccggcctg aagagaggaa gaccggcaat attggatttc tttttcttaa
     1981 gaacagccgg attcacaata tcattccctc caatgacaag acatacatct gcaatccgaa
     2041 tctcttcgtt ggaatggtct gtatccacta ttctgtcgta cggaatctct gcctctgaaa
     2101 gaagtacatt catatgcccg ggcattcgtc cggccagagg atggatggca aatttcacct
     2161 gtatttctct ttcttccaga agagtgacaa gttccttaca gacatgattt gcctgtgagg
     2221 cagcaaaacc atatccagga acgatgacta ctcttttggc aaaggccagg atgattgccg
     2281 catcagagac attgatttca ttgatgatta ctgcttcaaa gcttccggta tgctgctcag
     2341 attcctgttt tggagaaatc ccaagaaaaa ctgaaaaaag atttctgttc attgaaaatg
     2401 aaacctttct tgcgagcata aaccctgcag cacaggtcac agtcccggtc agtatcataa
     2461 tctgattctc agaatacagt ccaaaaagaa atgcagaaaa accgctcaca gaattcagtc
     2521 cagcaatgac aacaggcata tcagttccaa tgacagggaa cataataatc aatgcatata
     2581 aaaatgcaaa aaatgcagaa agggaaagaa gagcaaaatc ccgatctcct ttcagtagga
     2641 gactgaacat cagcactccc agaagaaaaa tgaacatcag attcagacca gtctgcatcg
     2701 gataagaata gttcctgacg ggtatccgga gtctgatttt tgctatgctg agaagatttc
     2761 cgctgaatgc cgccataccg ctcatcaggc ataaaatcag aataaatact gggaagtagg
     2821 ctcttgtact gggctccaga taaggagttc tggaaaattc aataaaggct accgctgaat
     2881 cggaa
//
LOCUS       DAOQGJ010000287         2875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21186_length_2875_cov_2.662411, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000287 DAOQGJ010000000
VERSION     DAOQGJ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21186_length_2875_cov_2.662411"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PK683_05805"
     CDS             complement(<1..180)
                     /locus_tag="PK683_05805"
                     /inference="COORDINATES: protein motif:HMM:NF040119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6493 family protein"
                     /protein_id="HNH07997.1"
                     /translation="MNKISIPDNWDDLLFYIGKVIKSDNTWSIEIFAEGLNQLQDSIP
                     ADFAKQIKPYRKQLEN"
     gene            complement(177..1409)
                     /locus_tag="PK683_05810"
     CDS             complement(177..1409)
                     /locus_tag="PK683_05810"
                     /inference="COORDINATES: protein motif:HMM:NF040119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6493 family protein"
                     /protein_id="HNH07998.1"
                     /translation="MESIKKLIKSENWKEILEYGVSLTDEQRYSVISELTQYTEKDRK
                     LLGNTYVNYLFIVCIRSYKDIEKTKYFNGFSALHWFINDAKIGSEPLEEYFRKFPPNY
                     MDRYLEDESRDRYFWQNFRIIWNLHKNGWIKFNENIFTVLFFTLYGFENSHIRDAEYL
                     IRNRDILEKVFLQFYKYEIPVLESIKCETIEYPNGLSVKAGVYWTEVFKILIEKKAVT
                     DRSIVRHLLGSLLNNWKKPHLVWLCGILELFKPSNSELLEYQNLIFSGFNTGNTQLIN
                     FSQGLAERISKEENFNYKSYISNVQSVFAVEKIDKSILGILSVIDYGIQKFPELKKGL
                     ADGLKILFTQTSEKVQLKTAEIMIQLEDEKGLKKTVSEYDSALKKKAKALLLDGGGSG
                     NTNTEKELKKIQKQGVRK"
     gene            1815..2375
                     /locus_tag="PK683_05815"
     CDS             1815..2375
                     /locus_tag="PK683_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH07999.1"
                     /translation="MDKLEYYRVREYAMYGEPAWWGILCLDRSTKKGRSLLLSGLQEN
                     LKIFLEVEKFSEEKKYSDSFVKNFSGTIMYMSEIEFPGKKKTADLEKKLFEWIDDKTS
                     KSFQKLSEDFQKYNAEVSAESYFKSVKKLEKFSTRDPWAYFRYYWSDKTKFGSNESFF
                     FSDEDSLYEKDVEDDGEPGSFELEKT"
     gene            2549..>2875
                     /locus_tag="PK683_05820"
     CDS             2549..>2875
                     /locus_tag="PK683_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNH08000.1"
                     /translation="MYQEKNWILLRVFLTDRDKYDGRPLYSEIVLKCRELGLKGATVL
                     RGLIGYGNSGVLHSAGILSMSDSLPLVLEVCDESDKIDSALPFFRKMMDESGCGGLIT
                     MEAARVI"
BASE COUNT          843 a          543 c          575 g          914 t
ORIGIN      
        1 attttccagc tgctttcggt aaggcttgat ctgttttgca aaatcggcag ggatactgtc
       61 ttgaagctga ttcagccctt ccgcaaaaat ttcaatactc caggtattgt ccgatttgat
      121 gacttttcca atataaaaaa gaagatcatc ccagttgtca ggaatactga tcttgttcat
      181 tttcttactc cctgcttttg aatctttttt aattcttttt ctgtatttgt attgccggac
      241 ccgcctccat ccagaaggag agcctttgcc ttttttttaa gggctgaatc atattccgaa
      301 acagttttct tcagaccttt ctcatcctca agctgaatca ttatttcagc agtcttgagc
      361 tggaccttct ctgaagtctg ggtgaaaagt attttgagac cgtctgccag accttttttc
      421 agttcgggga atttctgaat tccgtaatca atgacagaga gaatgccaag gatcgattta
      481 tcgatttttt caacagcaaa tacagactgt acatttgata tatatgactt ataattgaaa
      541 ttttcttctt tgctgattct ttcagcaaga ccctgagaaa aattgatcag ttgagtattg
      601 cctgtattga atcccgaaaa aatcagattc tgatattcca gaagttctga atttgaaggc
      661 ttaaaaagct ccagaatgcc gcaaagccag acgagatggg gttttttcca gttgtttaaa
      721 agggacccca gaagatgtct gacaatgctc ctgtcagtaa cggctttctt ttcaatgaga
      781 atcttgaata cttccgtcca gtaaacccct gctttgacac tcaacccatt tggatattca
      841 attgtctcgc attttatgga ttccagaacg ggaatctcat acttgtaaaa ctggaggaag
      901 actttttcaa gaatgtctct gttccgaatc agatactctg catccctgat atgactgttc
      961 tcaaaaccgt aaagagtaaa aaaaagaact gtaaatatat tttcattaaa ttttatccaa
     1021 ccatttttat ggagattcca tatgattcgg aaattctgcc agaagtacct gtccctggat
     1081 tcatcttcaa gatatctgtc catataattc ggagggaatt ttctaaaata ttcttcgaga
     1141 ggctctgatc caatcttggc atcattgata aaccagtgaa gtgcagaaaa cccattgaag
     1201 tattttgttt tctctatatc cttgtaagat ctgatgcaga cgataaaaag gtaatttaca
     1261 taagtgtttc ccagaagttt cctgtctttt tccgtatact gagtcagttc actgatgact
     1321 gaataacgct gctcgtctgt caatgagacg ccatattcca gaatctcttt ccagttttcc
     1381 gatttaatca gtttttttat agactccaaa ctgtctcctt aagtcattgc ggcaagggta
     1441 ctgcgctgca ttacactgct ttaaaacagg aaacagattc gtcggatctg ttttttcagc
     1501 aagccaaatt taccttttcg gaaatgaatt tcgcccggaa gtgttcaggt cagaaatact
     1561 ttcgttgtca tttatattga ttaccaaaag taattgcgct gagaatcttg atcttaccga
     1621 tccggcgaga ctgaaatatg aaaagacttt tgaatatgat tataaaagtc ttcatatttg
     1681 cgaatttaga aactcatctg ttttcgtcct gcggagaagt ctcatatatg aaatattcaa
     1741 tgacagtttc tgaatctgag gattctgcaa attgcttgca ttgcagaggg aagaaaattt
     1801 aataatctct tcatatggac aaacttgaat attacagagt cagagaatat gcaatgtatg
     1861 gagagcctgc ctggtggggc attctctgtc tggacagaag cacaaaaaag ggcaggtccc
     1921 tgcttctcag cggattacag gagaatctga aaattttcct ggaggtggaa aagttttcag
     1981 aagaaaagaa atactcagac agttttgtaa aaaacttttc cggaacaatt atgtatatga
     2041 gcgaaattga atttccaggg aaaaagaaga ctgctgatct ggaaaagaaa ctctttgaat
     2101 ggattgatga caaaacttcc aaatcatttc agaagctttc cgaggatttt cagaagtaca
     2161 atgcagaagt gtctgcagaa tcttatttta aatctgttaa aaaactggaa aaattttcca
     2221 cccgcgaccc ttgggcatat tttcggtact actggtcgga caagacgaaa ttcggatcca
     2281 atgaatcttt ctttttcagc gatgaggaca gtctttatga gaaagatgtg gaagacgacg
     2341 gtgagccggg ttcattcgag ctggaaaaaa cttaaatctt agattttata ttcaggccat
     2401 ttttcggctg aaatgttttc tgaatgcatt ttcatttaac agtttctgat tcaccctgtg
     2461 cttcagttct gcccaagact gcaggcggtc tgcggcgcaa ggtaaattag tagaacaatt
     2521 tatagaattt tttatcaaag gaacaaggat gtatcaggaa aagaactgga ttcttttaag
     2581 agtgtttctg actgacagag acaaatatga cggcaggccg ctgtattcag aaattgtact
     2641 gaaatgcaga gagcttggac tgaaaggagc aacagtgctt cgaggactga tcgggtacgg
     2701 aaattccggg gttcttcatt ctgcaggcat tctttccatg tcggactctc ttcctcttgt
     2761 gctggaagtc tgtgatgaat cagataagat agactctgct cttccttttt tcagaaaaat
     2821 gatggatgaa tccggatgcg gaggtctcat cactatggaa gctgcaagag tcatt
//
LOCUS       DAOQGJ010000288         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21224_length_2871_cov_3.682884, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000288 DAOQGJ010000000
VERSION     DAOQGJ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21224_length_2871_cov_3.682884"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..606
                     /locus_tag="PK683_05825"
     CDS             100..606
                     /locus_tag="PK683_05825"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNH08001.1"
                     /translation="METERRYNIKIEVYFPDAEKTEDMVREKGINLFYASVENRKLCC
                     AVRKLEPLERALNGARLWITGLRREQSVTRTQMNFAEWDEKYQTVKINPLIDWTWEKT
                     LAYIKENKVPYNPLHDQNFPSIGCAPCTRAVLEGENIRAGRWWWENPETKECGLHISD
                     GQIIRNKK"
     gene            603..1505
                     /gene="cysD"
                     /locus_tag="PK683_05830"
     CDS             603..1505
                     /gene="cysD"
                     /locus_tag="PK683_05830"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNH08002.1"
                     /translation="MKYHSTHLKQLEAEAIYIMREVAAQCEKPVLLFSGGKDSITLVR
                     IAEKAFRPAKFPFPLLHIDTGHNFPETIEFRDRLIAKTGEKLIVRYVQDSIDQGKAVE
                     EKGRNASRNKLQTVTLLDALEEFKFDAAFGGARRDEEKARAKERIFSFRDEFGQWDPK
                     KQRPELWNLYNGKIRKGESIRAFPISNWTEMDVWQYILMEGLEIPEIYFTHRRDCFYR
                     DGMLVPLSEYLKNDPEEKVENMQVRFRTVGDMTCTGAVLSSADSLEEIIQEVASARVT
                     ERGGRADDKRSEAAMEERKKEGYF"
     gene            1529..2782
                     /locus_tag="PK683_05835"
     CDS             1529..2782
                     /locus_tag="PK683_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH08003.1"
                     /translation="MDMLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIQASSER
                     RGDGYTNLALLTDGLKAEREQGITIDVAYKYFATPKRKFIIADTPGHIQYTRNMVTGA
                     STADLAVILIDARKGVIEQTLRHSFLTAMIGIPHLVICINKMDLVDFSETIYNDIQKH
                     YFEFASRLNIPDVRFIPISALNGDNVVERSKSTPWYKGETLLELLENIQVFEDMNLKL
                     PRFPVQWVIRPMSNEYHDYRGYAGTVASGIFKKGDKITVFPNGQKGTIKSIETFEGEI
                     EEAYPPLSVSILLNEELDISRGDIIASGDLPAAEQEFETYVCWMDTKPLNVNGKYLLQ
                     HTTASCKCIIKEILYKLDTNHLKRTEGGETLELNEIARVVIKTSKPIVFDDYAVNRPM
                     GSFIIIDEGTNNTSGAGIIRKKEIV"
BASE COUNT          985 a          557 c          658 g          671 t
ORIGIN      
        1 acagatttaa aaattttaaa ggtaaattag aaatttaaat gatgaaatta gaaacattta
       61 gaattaggct gagcattaaa taagactgct ataacaagaa ttgaaacaga aagaagatac
      121 aatataaaaa ttgaagttta ttttcctgat gcggaaaaaa cggaagatat ggtcagagaa
      181 aaaggaatca atctttttta tgccagtgtg gaaaacagga agctctgctg tgcagtgaga
      241 aaactggaac ccctggaaag agcattgaac ggtgcaagac tctggatcac agggcttcgc
      301 agggaacagt cagttacaag aactcagatg aatttcgcag aatgggatga aaagtaccag
      361 acagtcaaaa tcaatcctct catcgactgg acctgggaaa aaacactggc ttatatcaaa
      421 gaaaataaag ttccttacaa tcctctccac gatcagaatt ttccgagcat aggatgcgct
      481 ccctgcacaa gagcagttct ggaaggagaa aatatccgtg ccggcagatg gtggtgggaa
      541 aatcctgaaa caaaagaatg cggacttcat atttcagacg gacaaatcat acggaataaa
      601 aaatgaaata tcactctaca catttaaagc agctggaagc agaagcgatt tacatcatga
      661 gagaagttgc agctcagtgt gaaaaacctg tacttctttt ttcgggaggc aaggactcca
      721 tcactcttgt tcggattgca gaaaaagcat tccgtcctgc aaaatttccc ttccctcttc
      781 tgcacattga taccggtcac aattttccgg aaacaataga attccgtgac agactgattg
      841 ccaaaacagg tgaaaaactg attgtacgat atgttcagga ttcaatagat cagggaaaag
      901 cagtggaaga gaaaggcaga aatgccagca gaaacaaact tcagacagtc acactgctgg
      961 atgcactcga agaattcaag tttgatgcag cttttggcgg tgcaagaaga gatgaagaaa
     1021 aggcgagagc aaaggaaaga attttttcct tccgtgacga gtttggacaa tgggatccga
     1081 aaaaacagag acctgaactc tggaatctgt acaacggaaa aatccgtaaa ggcgaaagca
     1141 ttcgcgcatt tccgatcagc aactggactg aaatggatgt atggcaatat attttaatgg
     1201 aaggtctgga aattcccgaa atctacttta ctcacagaag agactgcttt tacagagacg
     1261 gaatgcttgt gcctctttca gaatatctga aaaatgatcc tgaggaaaaa gtggaaaata
     1321 tgcaggtgag attcagaact gtaggagaca tgacctgcac aggtgcagta ctttcttctg
     1381 cagattcact tgaagaaatc atacaggaag tggcaagtgc ccgtgttact gaacgcggcg
     1441 gaagagcgga tgacaaaaga tcggaagccg caatggaaga aagaaaaaaa gagggatatt
     1501 tctagtggac aacagacagt atctgagcat ggatatgctc cgcttcatca cagcggggag
     1561 cgtggatgac ggaaaaagca cactgatcgg aagactgctc tatgacagca agtccatatt
     1621 tgaagatcag ctcgaagcaa ttcaggccag ttcggaaaga agaggagacg gatatacaaa
     1681 tcttgctctc ctcacagacg gactgaaggc agaaagagaa cagggaatta caatcgatgt
     1741 ggcttataaa tattttgcca caccgaaaag aaaattcatc attgcagata ctccaggaca
     1801 catacagtat acaagaaata tggtgacagg cgcatctaca gccgatcttg ctgtcatact
     1861 catcgatgct agaaagggag tgatagagca gactctgcgc cattcatttc tgactgcaat
     1921 gatcgggatt cctcaccttg tgatctgcat caacaagatg gatctggtgg atttttccga
     1981 aacgatatac aatgacatac agaagcatta ttttgaattt gcaagccgac tgaatattcc
     2041 tgacgtaaga tttattccta tcagtgctct gaacggagac aacgtcgtgg aaagatcgaa
     2101 gtctactccc tggtacaaag gagagactct tctcgaactt ctggaaaaca ttcaggtatt
     2161 tgaagatatg aatctgaaac tgccccgttt tcccgttcag tgggtgatcc gtccgatgag
     2221 caatgaatat catgattaca ggggatatgc cggaactgtc gcaagcggaa tattcaaaaa
     2281 aggcgacaaa attactgtat ttccaaacgg tcagaaggga acaataaagt caattgaaac
     2341 ttttgaggga gagattgagg aagcataccc tcccctttcc gtttccattc ttctgaacga
     2401 agaactcgac atcagccgag gtgacattat tgcatcaggc gacctgcctg ccgcagagca
     2461 ggaattcgaa acttatgtct gctggatgga tacaaaacct ctgaacgtaa acggaaaata
     2521 tcttcttcag cacacgactg cttcctgcaa atgcatcatc aaggaaatcc tttacaaact
     2581 cgatacaaac catctgaaaa gaacagaagg gggcgaaact ctggaactga atgaaatcgc
     2641 cagagtagtg attaaaacct ccaagccgat agtttttgat gattatgcag tgaacagacc
     2701 gatgggatct tttatcatta tagatgaagg aacaaacaat acatccgggg caggaatcat
     2761 ccgtaagaag gaaattgtct gattggctga tctttccgga aaaaaatacc tcccgatgat
     2821 gatggatgtg actgaaaaat caattctcct catcggtgcc gggaatgcct g
//
LOCUS       DAOQGJ010000289         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21388_length_2858_cov_2.622904, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000289 DAOQGJ010000000
VERSION     DAOQGJ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21388_length_2858_cov_2.622904"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="PK683_05840"
     CDS             complement(<1..1313)
                     /locus_tag="PK683_05840"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNH08004.1"
                     /translation="MNGLTGTEAGKREISLSEPLCIKWKILSDPEFMEISSILQTLLN
                     QWDKLYLAETIMIILRELLINANRAIVKREFFLQRNYNLNSHYDKGMYEFREYILKNW
                     PNNARTIMEDSLYFVSLKISLNEDGMIFEIMDNCSLHPVEKSRIQSRIQAADKYRNIM
                     EAYKDISDSQESAGLGIIMILKLLNGVGLDSSHFELHEGKEETKFVISFPSEIIPLEI
                     STKMQERIIKEVEILPSLPQALTKIMDICRSPNMDMKKLVDEIEKNPAVSADVLKLAN
                     SAGFISRAKPNNLSLAVRKIGSNGVLKMLYAISSLKILNGRYGKMEAEWDHAGRTSFF
                     AGKLAKDFNYAKLLDSVVVGSLLHDIGKILLLSVDSELMSEILLLTKNRATENTKVLE
                     ESTIGVSHSKLGAMLAKKWNFPEDLSACIEFHQQPYMAPEEYRTHA"
     gene            1748..>2858
                     /locus_tag="PK683_05845"
     CDS             1748..>2858
                     /locus_tag="PK683_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNH08005.1"
                     /translation="MVTIQNDNLNNPFIGEDNCVPHFQPIVSVESRKIVGYEVLGRLF
                     SPEKSQYFSLGAFFHNRNTSFSERVRIDRIIREKAIRYWKETGIQGVNLFFNLMPNIL
                     SSLHQEDLLDINRFHLAQLINKYEINKSDVVIEITEDEFNGKQERLISMIELYKANGF
                     RIAVDDVGTGFSNLERIGYIHPDIIKVDIKIMRESLNKNSFRQVLLAVSEMASKLGSE
                     LLFEGVETEKELNLAFSMGASLIQGFYFSASCPEFINRETFSAKLQDSIEKYSGLHFM
                     EVVEKNMKSAQLIESILQAVNESSFSHSSVTERPDEILPALPATVYKAFLLDLNGNQI
                     SPTYFRSGEENWSTSYKDIGNNLSWKPYFIRHKAKN"
BASE COUNT          865 a          563 c          560 g          870 t
ORIGIN      
        1 gcatgggtcc tgtattcttc cggagccata tagggctgct gatgaaactc aatgcaggca
       61 gacagatctt ccggaaaatt ccatttcttt gcaagcattg ctcccagttt ggaatgactg
      121 actccgattg tagactcttc aagaactttt gtattttcag tggcgcggtt cttggtcaga
      181 agcagaatct ccgacataag ttcagaatca actgagagca ggagaatttt tccaatatcg
      241 tgaagaagag aaccgacgac taccgaatcc agaagtttgg cataattaaa gtctttggcg
      301 agtttgcctg caaaaaaact tgtcctgcct gcgtggtccc attctgcttc catcttccca
      361 tatctcccat tcaggatttt aagagaactg attgcataga gcatttttag gacaccattg
      421 gagccgattt ttcggacagc caaggaaaga ttgttcggtt ttgccctgct gatgaaaccg
      481 gctgaatttg caagcttcag tacatcagca ctgaccgcag gatttttctc aatctcatcc
      541 acaagctttt tcatatccat attcggagat cgacaaatat ccataatctt tgtcaaagcc
      601 tgcgggagtg atggaagaat ctccacttcc ttaatgatcc tctcctgcat tttggtactg
      661 atttcgagtg gaatgatttc gctggggaaa ctgataacga atttagtttc ctctttacct
      721 tcatgaagct caaaatgtga actgtccaaa cctacaccgt tcaatagctt tagaatcata
      781 ataattccaa gaccggcact ttcctgggag tcagaaatat ctttataagc ctccataata
      841 tttctatatt tatcagcagc ctgaatcctg gactgaatcc tggatttttc caccggatga
      901 agagagcagt tgtccattat ttcaaagatc attccgtcct cattaaggct gatcttgaga
      961 ctgacaaagt acagagaatc ttccatgatt gttctggcat tattcggcca gtttttcaga
     1021 atgtattctc taaattcgta catcccttta tcataatgag agttcagatt ataattgcgc
     1081 tgaaggaaaa attctctttt tacgattgct ctgtttgcat tgatgagcag ttccctaagg
     1141 ataatcataa tggtttctgc aagatacagt ttgtcccatt gattcaggag agtctgcaga
     1201 atagaggaaa tttccataaa ctccgggtct gatagaattt tccattttat gcacaaaggt
     1261 tcactcagtg atatttctct tttccctgcc tctgtgccgg tcagcccgtt catacagaca
     1321 aagtaaaaat cttttaaaac aattgcaagc tgataaatac agaattaaac gccgtacagt
     1381 aagatttttt tcaaaaagat acagacagca ttaaaaactt tttaagatta tagattagtt
     1441 atggaaatgt cgagacaggg aacgcagagt ctctttttat ggagctcagt acatatgcag
     1501 aacagaacat tgtaaatcat atgccctttg cagtaagatt cagccgctgc cgttgtatat
     1561 ttaaacataa atcggtaatt tttttgaaaa taaatgtatt tttaatagtc tctcaattta
     1621 aatttggaca gaatgaccgg tcagtcagtc gttatataca gaagagaaga tcttaaattt
     1681 ggacggtttt tcggtttttc tcttgacaga cattaaaaac atttgaagtt acattgagta
     1741 caaataaatg gtaaccatcc agaatgacaa tctgaacaat ccttttatag gtgaggacaa
     1801 ctgcgttcct cattttcagc caattgtcag tgtagagtcc agaaaaattg tcggatatga
     1861 agttttggga agactttttt ctcccgaaaa atctcagtat ttttccttag gcgcattttt
     1921 tcataatcga aataccagtt tttctgaacg ggtacgcata gacagaatta ttcgggaaaa
     1981 ggcaatacga tactggaagg aaacaggcat tcagggggtg aatctgtttt tcaatctgat
     2041 gccgaacatc ctttcgagtc tgcaccagga agatcttctg gatatcaacc ggtttcatct
     2101 tgctcagctt atcaataaat atgaaataaa taaaagtgat gtagtaatag aaattacaga
     2161 agatgagttc aacggaaagc aggaaaggct tatcagtatg attgaactct acaaagcaaa
     2221 cggattcagg attgcggtgg acgacgtagg cacaggcttt tcaaatctgg aacgcatcgg
     2281 ctacattcat cctgatatca ttaaagttga tatcaaaata atgagagaat cattaaataa
     2341 aaattccttc cgtcaggtgc ttctggcagt atctgaaatg gcatcaaagc ttggttcaga
     2401 gcttcttttc gaaggtgttg aaacagaaaa ggaactgaat cttgcatttt ccatgggtgc
     2461 gagtctcatt cagggatttt atttttctgc ctcgtgccct gagttcatca acagagagac
     2521 attttctgcc aaacttcagg attccattga aaaatacagc ggacttcatt ttatggaagt
     2581 agtagaaaaa aatatgaaat cagctcagct gattgaaagc atacttcagg cagtcaacga
     2641 gtcttcattt tcccattctt ctgttaccga aaggccggac gaaatactcc ctgctcttcc
     2701 tgccactgtc tacaaagcat tccttctgga tttaaacggc aatcagatca gtcctacata
     2761 tttccggagc ggggaggaaa actggagtac ttcctacaaa gatattggca ataacttaag
     2821 ctggaaacct tattttatcc ggcataaggc aaagaatt
//
LOCUS       DAOQGJ010000290         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21402_length_2856_cov_2.988218, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000290 DAOQGJ010000000
VERSION     DAOQGJ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21402_length_2856_cov_2.988218"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..312)
                     /locus_tag="PK683_05850"
     CDS             complement(52..312)
                     /locus_tag="PK683_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HNH08006.1"
                     /translation="MKTKASVITLRVPHELKHKIEKAADEQGISINQLALYAFTKELA
                     ELETSEFFRKYLKGQKKEKILSDFDTVMKKVKKRKVPDWDKI"
     gene            complement(302..742)
                     /locus_tag="PK683_05855"
     CDS             complement(302..742)
                     /locus_tag="PK683_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNH08007.1"
                     /translation="MTVVLDTNVIYQALQSASGASHYILRLVREQKIRLAVSVPVFSE
                     YEAVLKREESLKDFDLTKSDIDAFLRFIAYIAKPYTIFFLFRPNLRDENDNIFVELSL
                     TANADCLITGNIKDFTKNRNLNFEDLKILTPSEFVRNWRKENED"
     gene            910..1041
                     /locus_tag="PK683_05860"
     CDS             910..1041
                     /locus_tag="PK683_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08008.1"
                     /translation="MRNLEKTLKKMEKKASDIWKNWVTVIRNELNSRPIKSEEWKQN"
     gene            1067..1954
                     /locus_tag="PK683_05865"
     CDS             1067..1954
                     /locus_tag="PK683_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH08009.1"
                     /translation="MIRRNLKTLLWILWFVLTVSLTAWWTILAHRQASGIAELQRKLQ
                     SEYSEQTAEKMQRQERMIRMEGAFFLALITTAGVALVVLSLREERRARAMREFFAAMT
                     HEIKTPLASLRLQAESLQDSLKNKRDKVLAERLIRNTERLELQMENAIFLSSLDRKNH
                     LYIENINIYNFFQSLSFGYREVRIQAEQESSVRADRRALEIIFRNSIENSIQHGKASE
                     VILGCQTEKGRTVLFIEDNGLGFQGDWKRLGQLFYRHRSGSGTGIGIYIMKILAKKIQ
                     SDIYFIRKEKGFRTEVVFQ"
     gene            complement(1948..>2856)
                     /locus_tag="PK683_05870"
     CDS             complement(1948..>2856)
                     /locus_tag="PK683_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH08010.1"
                     /translation="VAYYPAAIAKEMNLNEESIDQIKEAAILHDIGKIIIPDSILLKP
                     GKLSFKEYELIKKHLDVGVEILNKIDYYKPLAEIIKFHHEKYDGSGYPYKMKDDEISI
                     LSHIMIVADAFDAMTTNRIYQARKTVKEAADELMRYRGKWYHPDVVDAAVKISQDEKF
                     AETATSQLPFTQMERERFAYFFKDQLTGLYNNTYLGLILNNLIPNLAYHYFVLIELHG
                     MTVYNEEKGWHQGNIILQKFSELISKMARDEQIFRVFGDDFIIGFETENQADAFLKNW
                     VPCQIETICATCEKIEKDKFRDYIDH"
BASE COUNT          806 a          572 c          618 g          860 t
ORIGIN      
        1 gatctgtgca taaagtcatg ctcagatttt cagtctgagc tgtaaaaaaa attatatctt
       61 atcccaatcc ggaacttttc ttttttttac cttcttcatc acagtgtcga aatcactcag
      121 tattttttcc tttttctgtc ccttcagata cttgcggaaa aattcagaag tttccagttc
      181 tgcgagttcc tttgtgaatg catacagtgc cagctgattg atggaaatcc cctgttcgtc
      241 cgcagctttt tcgattttgt gcttgagttc atgaggaact ctgagagtga tcacgcttgc
      301 cttagtcttc attttccttc ctccagtttc tgacaaattc agaaggtgtc agaattttca
      361 ggtcctcaaa attgagattc ctgtttttag taaaatcctt tatatttccg gtgatgagac
      421 agtccgcatt tgcagtcaga ctcagttcca cgaatatatt gtcgttttca tctctcagat
      481 tcgggcggaa aagaaaaaat atcgtatacg gttttgcaat gtacgcgata aacctgagga
      541 atgcatcgat gtctgattta gtcagatcaa aatccttcag actttcttct ctcttcagta
      601 cagcctcata ctcggaaaac accggcactg aaactgcaag cctgattttc tgctctctca
      661 caagtcttaa aatgtaatgc gaggcaccgg atgcactctg cagtgcctga tagatcacat
      721 ttgtatccag tacaactgtc acaaagcaat ggtagcatat atgctgtcat tgtcaatcgg
      781 aatttttcct ttgctggcaa ggactcaaat gcttttcgat tcttccttta tcctcaaaat
      841 atccttgcag gattggaatc tctgttaaaa ttgaagtgtt atggaggctc tttttttggg
      901 ggatgcggta tgagaaacct ggaaaaaacc ttgaagaaaa tggaaaagaa ggcatcagac
      961 atctggaaaa actgggttac agtcattcga aatgagctga attcccgtcc tattaaatca
     1021 gaagaatgga aacaaaattg aaaatcatcc cgtccggtaa aaaatcatga tccgtcgaaa
     1081 tttaaagaca ctgctttgga tactctggtt tgttctgaca gtctcactga ctgcatggtg
     1141 gactatactt gcgcataggc aggcatccgg gattgcggaa ctgcagagga agctgcagtc
     1201 agaatacagt gaacagactg ctgagaagat gcagagacag gaaagaatga tccggatgga
     1261 aggggctttt ttccttgcac tgatcactac cgccggagtg gcactcgttg tattgagtct
     1321 gcgcgaagag agaagggcgc gggcgatgag agagtttttt gctgcgatga cccacgaaat
     1381 caagacccct ctggcatccc tccgtcttca ggcggaaagt cttcaggact ctctgaaaaa
     1441 caaaagagac aaggttcttg cggaaaggct gatcagaaac acggaaagac tggagcttca
     1501 gatggagaat gcaatattcc ttagctcact ggacaggaaa aaccatctgt atatagaaaa
     1561 tataaatata tataattttt tccagagtct gagcttcgga tacagggagg ttcggattca
     1621 ggcggaacag gaatcttctg tgagagcaga cagacgggct ttggaaatca ttttcagaaa
     1681 ctcgatagag aattcaattc agcatggaaa ggcatctgaa gtgattcttg ggtgtcagac
     1741 ggagaaggga aggacagttc tcttcattga agacaacgga ctgggatttc agggagactg
     1801 gaaaagactg ggacagcttt tttacaggca cagatccggg agcggaacag ggataggaat
     1861 atacatcatg aaaatccttg caaaaaaaat tcagtctgat atatatttca tccgaaaaga
     1921 gaaagggttc agaactgagg ttgtttttca gtgatcaatg tagtcccgaa acttgtcttt
     1981 ttcaattttt tcgcaggtgg cgcagattgt ttcaatctga cagggtaccc agtttttcag
     2041 gaatgcatct gcctgatttt cagtttcgaa accgatgata aaatcatctc cgaatacacg
     2101 gaatatctgt tcatctctgg ccatttttga aatcagctca gaaaatttct gaagaatgat
     2161 atttccctga tgccagcctt tttcttcatt gtacacagtc attccgtgca gttcaatcag
     2221 tacaaaataa tgatatgcca gattgggaat cagattgttc agtatcagcc cgagataggt
     2281 gttgttgtag agtcctgtca gctgatcctt gaagaaatag gcaaaccgtt ctctttccat
     2341 ctgagtgaaa ggaagctggg atgttgcggt ttctgcaaat ttttcgtcct gagaaatttt
     2401 aacagcggca tccactacat caggatgata ccatttccct ctgtatctca tcagttcatc
     2461 agcggcttcc tttacggttt ttcttgcctg gtagattcgg tttgtggtca ttgcatcgaa
     2521 tgcatccgcg acaatcataa tatgcgaaag gatggaaatt tcatcatcct tcatcttata
     2581 gggatagccg gaaccgtcat atttctcgtg gtgaaacttt ataatttcag caagaggctt
     2641 ataataatct attttattta atatctccac acctacatcc aggtgttttt taatgagttc
     2701 atattccttg aaggaaagtt ttccgggttt cagcagaatg gaatccggaa taatgatttt
     2761 tcctatatca tgaaggattg cagcttcctt tatctgatca atgctttctt cattcagatt
     2821 catttccttt gcaatcgctg ccggatagta ggctac
//
LOCUS       DAOQGJ010000291         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21416_length_2854_cov_2.756699, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000291 DAOQGJ010000000
VERSION     DAOQGJ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21416_length_2854_cov_2.756699"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..560)
                     /locus_tag="PK683_05875"
                     /pseudo
     CDS             complement(96..560)
                     /locus_tag="PK683_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004506879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
     gene            complement(641..1471)
                     /gene="motB"
                     /locus_tag="PK683_05880"
     CDS             complement(641..1471)
                     /gene="motB"
                     /locus_tag="PK683_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNH08011.1"
                     /translation="MAKQRCPECIQKVPEYMLTYGDMVTLLLCFFVMLYTTGKTNQRE
                     MQIILSVFKSSTGFFDGGQTLSKGSLEQMGMNVESLPSQTRGRASSKAIKAAHQVFKS
                     EMQQGKVRVSEDERGLVISLMGADYFNPASAILLPPIKDVLKKAAPFIKEMDRYVRVE
                     GHSDHDAVLPGTNEGKEERNYINNWDLAAARAVNTTTYLVNVGQLDPEILQAVSYGSA
                     RPFAVEMDSNIPEAKAYNRRIDLVLLSEKSVKRKISESNYKLPKSKLPGTENTLEGEN
                     "
     gene            complement(1475..2266)
                     /locus_tag="PK683_05885"
     CDS             complement(1475..2266)
                     /locus_tag="PK683_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HNH08012.1"
                     /translation="MRSFDIATFIGLAAGLFVLTLGVIVAGLNPLTIIDIPSILITIG
                     GGIASTIISAPWEGTLNIGKVTRKIFFVTEFNTTGLINTLVSFSEKARREGLLALEDD
                     VSELSDQFLKKGIQLVVDGTDPELVRNIMETEMGNIAGRHANGRNWWDTLGSLAPGFG
                     MLGTLIGLIAMLQNLGSGDTTAIGIGMAAALITTLYGSFVANLLAIPFVKKLIRKSED
                     ELAMKQIMIEGTLSIQSGDNPRIVKEKLSSFLSPSERASLAEDKD"
     gene            complement(2266..2478)
                     /locus_tag="PK683_05890"
     CDS             complement(2266..2478)
                     /locus_tag="PK683_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNH08013.1"
                     /translation="MHRLNKTELVINASQIETIESTPDTVITLINEKKFIVSESLDEV
                     VNNVIKYHRTIFQSAFSGFRSEGEKQ"
     gene            complement(2518..>2854)
                     /locus_tag="PK683_05895"
     CDS             complement(2518..>2854)
                     /locus_tag="PK683_05895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004475292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH08014.1"
                     /translation="FLSHLPTEQVVQFMKKAKGFIFPGLEDFGIAPVEVQGYCTPVLA
                     FGQGGALETVVDGKTGILFNEQTADSLNFGLERLERTEFVKKDFQDSISRFTEEKFIN
                     QMKSLVENV"
BASE COUNT          758 a          687 c          566 g          843 t
ORIGIN      
        1 accggaccgg acggcagtcc ggtcaatgag ctttttcaac aagtgaaaat aaaaatccag
       61 gcgacattaa gtctgcctgg caagagaaag agaaatcagt ttacaatgaa ctctctgaag
      121 tagacttcct taattttccc gtttgtcaga atatggttga tatgagcctt gatttcttcc
      181 tgcaggtcga ttttgtctgc tgcagtcttg agctcttcct tattctttct ggaaataatc
      241 aggttgatga tgttctgcat ctgagcctgt cttgtggaaa gctcctgagt cagggtcggc
      301 tgcccggttt ccacaccgaa agaaagcttc agtttgataa agtgaacttc acccacatca
      361 gatgtattga ttctgaattc ttccggaaaa ttgaacacat ccagcggagg aggagctttt
      421 acaagagcga tatttttctg ctgcttataa acagcattcg cagttctctg ggccacaaac
      481 atggacacaa ctgctacaat aatcactcca agtatacctg ccgccgcata catcagccat
      541 tttataattg gcgatttcgt ggccggggct acctcgacct tcggttcatc ctcatcattt
      601 aagttcgctg caccatcacc cattgggttt tcctcctgaa ttaattttca ccttccagtg
      661 tattttctgt accaggaagt tttgatttag gcaatttgta gttgctctca gaaatcttgc
      721 gttttacaga tttctcagac agaagaacca aatcaatcct tctgttatag gcctttgctt
      781 ccggaatatt ggaatccatc tcaacggcaa acggtcttgc tgaaccgtag ctcactgcct
      841 gaagaatttc cggatcaagc tggcctacgt ttactaaata tgtcgtcgta ttgactgctc
      901 tggcagcagc caaatcccag ttgttgatat aatttctttc ttcttttcct tcatttgttc
      961 caggaagaac tgcatcgtgg tcactgtgac cttcgaccct tacatatctg tccatttcct
     1021 tgatgaaagg agctgccttt ttcaatacat ccttgatcgg cggaagaaga atagctgaag
     1081 caggattaaa atagtctgca cccatcagag agataacaag tcctctttca tcttcggaaa
     1141 cccttacctt tccctgctgc atttccgatt taaaaacctg atgagccgcc ttgattgctt
     1201 ttgaactggc acgtcctctg gtctgggaag gaaggctttc cacattcatc cccatctgtt
     1261 ccaaacttcc cttggagaga gtctgccctc cgtcaaaaaa acctgtggag gatttaaata
     1321 cggagagaat aatctgcatt tctctctggt tggtctttcc tgtagtgtac agcatgacga
     1381 aaaagcagag aagaagggtc accatatccc cgtatgtaag catgtactcg ggtactttct
     1441 gaatacattc aggacatctc tgctttgcca tagtttagtc cttatcctct gccaatgaag
     1501 ctctttcgga gggtgagagg aagctggaaa gtttttcttt tacaattcta gggttgtctc
     1561 ctgactgaat ggaaagagtt ccttcaatca taatctgctt catcgcaagt tcatcctcgc
     1621 ttttgcggat gagttttttt acgaaaggaa tcgcaagcag gttggccaca aaagacccgt
     1681 acagggttgt aatcagggcc gccgccatac cgataccgat cgccgttgta tctcccgaac
     1741 ccaggttttg gagcatcgcg atgagtccga tcagtgttcc caacatcccg aaaccgggag
     1801 ccagacttcc gagtgtatcc caccagttcc ttccgttcgc gtgacggccg gcaatgtttc
     1861 ccatttccgt ttccataata tttctgacaa gctcagggtc agttccgtcc acaacgagct
     1921 gaatcccttt tttcaaaaac tggtcggaaa gttcactcac atcgtcttca agagcgagaa
     1981 gaccttccct tctggccttt tcagaaaaag aaaccaaagt attgatgagt cctgtagtat
     2041 tgaattctgt gacaaaaaag atttttctgg tcacttttcc gatattcaga gttccttccc
     2101 agggggcaga aataattgtg gatgcaatcc ctcctccgat cgtaatcaga atggaaggaa
     2161 tgtcaataat ggtcaaggga ttcaaacctg cgacaataac ccctaaagtc aaaacgaaga
     2221 gacctgcagc aaggcctata aatgttgcta tgtcaaatga acgcattatt gcttttcccc
     2281 ctcagaacga aaccctgaaa atgccgactg aaatattgta cggtgatatt taatcacatt
     2341 gttcactact tcatcaagag attcgctgac aatgaacttt ttctcattaa taagtgtaat
     2401 cacagtatca ggtgtagact caattgtctc tatctggctc gcattaatga ccagttccgt
     2461 tttattcaat ctatgcacag ttatcaaaat tattcctgaa aactaaaaaa ggttctctta
     2521 tacattttcg accagcgact tcatttgatt tatgaatttt tcttcagtaa acctggatat
     2581 gctgtcctgg aaatcctttt ttacgaattc tgtcctctcc agcctctcca gaccgaaatt
     2641 cagagagtcc gcagtctgtt cattaaataa aataccggtc ttcccgtcca caactgtttc
     2701 cagtgctccc ccctgtccaa atgccagaac aggagtacaa tagccctgca cttccaccgg
     2761 agcaatgccg aaatcctcca aaccagggaa aatgaaacct tttgcctttt tcataaactg
     2821 aactacctgt tcggtcggga ggtgagaaag gaat
//
LOCUS       DAOQGJ010000292         2853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21427_length_2853_cov_4.111866, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000292 DAOQGJ010000000
VERSION     DAOQGJ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21427_length_2853_cov_4.111866"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1359
                     /locus_tag="PK683_05900"
     CDS             70..1359
                     /locus_tag="PK683_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNH08015.1"
                     /translation="MQIKFISGSEELLNIFQREISSCHSFRMAAAWGSTAKGKGRHWE
                     ILKSNMDKLQEAILGIHFHQTEPEILFELHKWRKLNIVYDAKGVFHPKFYLFDKGDSF
                     SIISGSSNFTAGGFGKNIECNIFIQDDKKSSLYLEAEGFYSLCRQMSRIPDNLDLEQY
                     KEDYNSKISSNVDLTTFIASEERKQIFTRPGDGLPDYSWEGIFQFTWDEYVHGLKYLD
                     RIKYRGGMFFQKNIAESFRRDVLFSIDCFQKILHEQKFLTLKEMPEEERKAFCGTIAI
                     SQKYGNCGWLGSMTGAGYFKNRVIEHPETLDEHLNKISLEGNISDSTLLDYFTGILSL
                     ENVGQAGAVRLLAVKRPDLFVPINKANIESLIKLTKLKNLKYNSARKSDFIEAYLLLL
                     DKIYQSPWYKHSEPTEGIESKIFAYRAALLDSFVYEF"
     gene            1975..>2853
                     /locus_tag="PK683_05905"
     CDS             1975..>2853
                     /locus_tag="PK683_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HNH08016.1"
                     /translation="MSAQEVSKNISIVKFQPYKSSRQDTVADSIQSKLISELQKQGFA
                     AQSSSDENISAALNRAKTEKPYLTVSGYYEKNEHGTLNLYLQVYDSEKGTLIDSIIIA
                     DAYSGLEGVKLDKEEMSESDESRIDKFVKKAALKIKNNPKKKTQPEDLSDLKESPLAK
                     AVSFPFPAEEIEKQSAEVFQFLQETNINRQVEVASLFKEDELKVGSTVAVMDESQWKK
                     YGSRRANEPLSFLPSIMNNNTLGGAQATSIRGYTNVLSFTGVAKLIDGVPINNFLQGS
                     GVSNVANFGLGNTNRIE"
BASE COUNT          878 a          503 c          624 g          848 t
ORIGIN      
        1 ctctgcgatc agtttttgga tactttttat cttcgttttg agaataaaat ggacaaggga
       61 gaattttaaa tgcaaatcaa atttatttca ggttcagaag aacttttgaa tatttttcaa
      121 cgggaaatct caagctgtca ttcattcaga atggcggcgg cctggggttc cactgcgaaa
      181 ggaaaaggca gacactggga gatcctgaaa tcaaatatgg ataaactgca ggaagcaatt
      241 cttgggatac atttccatca gacggaaccg gagattcttt ttgagctgca taaatggaga
      301 aagctgaata ttgtgtacga tgcaaaaggg gtttttcatc ctaagtttta tctatttgac
      361 aagggagatt cattttctat aatttccgga agttctaatt ttacggctgg aggatttgga
      421 aagaatatag aatgcaatat ttttatacag gatgataaaa agtcttctct gtatctggag
      481 gcggaaggat tttacagtct ttgcaggcag atgtctagaa ttcctgataa tcttgatctg
      541 gaacaatata aagaagatta taattctaaa atttcatcaa atgtcgattt aactacgttt
      601 attgcttctg aggagaggaa acagatattt acaaggccgg gagacggtct tcctgattat
      661 tcctgggaag gaatttttca gtttacctgg gatgaatatg tgcatggtct gaagtatctg
      721 gacagaatca aatatagagg cgggatgttc tttcagaaaa acatagcaga aagcttcaga
      781 agagatgttc tattcagcat agattgcttt caaaaaattc ttcacgaaca gaaatttctt
      841 actttaaaag agatgcctga ggaagaaaga aaagcctttt gcggaacgat tgcgatttct
      901 caaaaatatg ggaactgcgg ctggcttggt tcaatgacgg gagcaggcta tttcaaaaac
      961 agagttattg agcacccgga aacactcgat gaacatttga ataagatttc actggaagga
     1021 aatatttccg atagtacact tctggattat tttacgggaa tactttcctt ggaaaacgtc
     1081 ggtcaggccg gggctgtgcg cctacttgca gtgaaacgtc cagatctgtt tgtcccaatc
     1141 aacaaggcaa atattgaatc cttaattaaa ctgactaagc tgaaaaatct gaaatacaat
     1201 tctgcaagaa aatctgattt cattgaagca tatcttcttc ttttggataa gatataccag
     1261 tccccttggt ataaacattc tgaaccgaca gagggtatcg aatcgaaaat ttttgcttat
     1321 cgggctgctc ttctggattc gtttgtgtat gagttttgaa agttcctgct gattgaattc
     1381 cgtccataga tcaggaattt tatgaaagta tatattataa ttgttatgat caaatgaaag
     1441 aattgtatgt tagggaattc ctgatatact catattcaaa agtgtttgct tattcccgga
     1501 tttccaatcg agcgcagcag cggtatttct ctgtcacagc agctgacgcg ggggctgctt
     1561 aatggcagtc gaacagtgtg gataggtcat tctttccttc ggcaaaacca gtatatgccg
     1621 gctgttcaca aaaacaagcc ttccgatttg cagacaggtc tattgtaaaa ctgacaccgc
     1681 tgacgggata tatgattttg aattcgtatg acgaaacgaa acattttttc ctgaatagaa
     1741 atctcattca ggtgatcgaa tttcctgcaa tgctgcaggc agactgaatt gtccttcggt
     1801 ctctcggatc ccttttttca tctagttctg cttcgccgct ccagttcaaa aaattattcc
     1861 tgattttaat aaaatttctt gccagaatga ctgccatact taatggtctt catttacatt
     1921 atgagataca gaatcatttt ttttatatta tcctttatta tttcagcagg ggggatgtct
     1981 gcgcaggaag tcagtaaaaa tatttcaata gtcaagtttc agccttataa atcttccaga
     2041 caggacactg ttgcagactc cattcagtca aaattgattt ctgagcttca gaagcagggt
     2101 tttgctgcac agtcttcgtc tgatgaaaat atttctgcag ctttaaacag agccaaaaca
     2161 gaaaagccat atttgacagt gagcggctac tatgaaaaaa atgaacacgg gacactgaat
     2221 ctttatcttc aggtttatga ttctgaaaaa ggcactttga ttgattcgat tatcattgcg
     2281 gatgcatatt ccggacttga gggagtgaaa ctggacaagg aggagatgag cgaatcggat
     2341 gaatccagaa ttgataaatt tgtaaaaaaa gctgcattaa agattaagaa caatccaaag
     2401 aaaaaaacac agccggagga tctttccgat ttgaaggaat ctcctctggc caaggcagtc
     2461 agtttccctt ttccggcgga agaaatcgaa aagcagagtg ctgaggtgtt tcagtttctg
     2521 caggaaacaa atatcaacag gcaggtagag gtggcttctc ttttcaagga ggatgaactg
     2581 aaagtcggct ccactgttgc ggtgatggat gagtctcaat ggaaaaaata cggctcaaga
     2641 agagcgaatg aaccattgag ctttcttcct tctataatga acaataatac tctcggcggg
     2701 gcacaggcga cttccattcg agggtacaca aatgtgcttt catttaccgg ggtcgcaaaa
     2761 ctgatcgacg gagtgcctat caataatttt cttcaaggct ccggggtttc gaatgtggcg
     2821 aatttcggac tgggaaatac aaacaggata gaa
//
LOCUS       DAOQGJ010000293         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21471_length_2849_cov_3.188976, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000293 DAOQGJ010000000
VERSION     DAOQGJ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21471_length_2849_cov_3.188976"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PK683_05910"
     CDS             <1..683
                     /locus_tag="PK683_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH08017.1"
                     /translation="LGRHAEPGVQIQDLPPLHSIIVSHAHLDHLDASTLKKLDKSAHL
                     ILPKGVSKDFSDYGFAKTSYVHWGDTVEESGMMIHSVRAQHFGGRWLIDNFWDGDPYT
                     SYIIEYKGITVYFAGDTGYSQEMFQEIGNRFKIDLALIPVGPFRFSGTSGLGNSVHVN
                     PNGALKMFDDLKAKWMVPMHFGTFYSPPEQELEVIKNAIQSSPKKDSVFLLGQGESKD
                     FFTQKGKE"
     gene            685..1599
                     /locus_tag="PK683_05915"
     CDS             685..1599
                     /locus_tag="PK683_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08018.1"
                     /translation="MKNGRPDQLEILEKENELLREKEMAQKDLLSFISHTFRNALSNG
                     QETVRQTLSLAEKAIENEYSEKLSYKAINNIAGLYSTFSMIDSLLDTFRMYGLHDEEF
                     MEEWSKNFDGDTDVNYLFAYALRQNCSRLLFSDEFREERYRLFNTQDPEIIRSKRKSF
                     LLEVLSLDLQKENAEIILSWFTDNAPFVELKIHDSITSFNTRKFHYNLIFSIITETMF
                     NAFKYSDGTVPVKISWKRERNRIEYSCKNQFSAESIRYSDTKSGLRFIEYLVRILKNV
                     EFVRTRKNSVSAAGLSIELMPSQSRGEE"
     gene            1596..>2849
                     /locus_tag="PK683_05920"
     CDS             1596..>2849
                     /locus_tag="PK683_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08019.1"
                     /translation="MKLLWVEDQPNEDLAGSLFRELLLKTFDEEFDPDNDIRDELPGF
                     FISPEYGISVCLSFHSFVNLFFRFRFDFDLFIIDINLSAKEPVSSDLPEGTPDRPAFL
                     KKAGFYIWNLLVRNGVPEDSIAFMTGNTDSIQEFNAMCISSSIPKLKDENVFDKSETG
                     YEKFRNWISEKKNSRYIQLRRGILEGIQYHLDSLEKNTSEILFNVTVRNDGTAEPVDT
                     DYASLYLEKLRFYFQKEYSEEPLRMYSFLTALSSEWERSYIHSSYTDSDNPFSSRHTP
                     AEEKFFHYTGFWIMKNLRNAIAHSCLEDSLLERDIAFFFIIAMRTFFKADLAAVYDYE
                     KILFAAMAEEKILNKSIRELQERSMNHILNLCYRKPKEGRMAEFKKIGNRFLESHKKV
                     FSRNRHYEEVPEFTKDLSRDDIRDNS"
BASE COUNT          905 a          559 c          619 g          766 t
ORIGIN      
        1 cactgggaag acacgcagaa ccgggagttc agattcagga tcttcctcca ttacattcaa
       61 taattgtttc gcacgcacat ttggatcatc tggatgcatc cactttgaag aaactcgata
      121 aatccgctca tctgattctg ccgaaaggag tatcgaagga tttttccgac tatggattcg
      181 caaagaccag ttatgttcat tggggagaca ctgttgaaga gtctggaatg atgattcatt
      241 ctgtccgtgc acagcatttt ggaggaagat ggctgattga caatttctgg gacggagatc
      301 cttatacaag ttatataatt gaatataaag gcattacagt gtatttcgca ggggatactg
      361 gttacagcca ggaaatgttt caggaaattg gaaacagatt caagatagat ctcgcactga
      421 ttcctgtcgg acctttccgt ttttcaggaa cctccggact gggcaattca gtgcatgtga
      481 atccgaacgg agcacttaaa atgtttgatg atctgaaggc aaaatggatg gtcccgatgc
      541 atttcggcac attctactct cccccggaac aggaactgga agtcataaaa aatgcaattc
      601 aaagcagccc gaagaaagat tcagtattcc tgcttggaca gggagaaagc aaggacttct
      661 ttacacagaa aggaaaggaa tgaaatgaaa aatggaagac ccgatcagct ggagattctg
      721 gaaaaggaaa acgaacttct cagagaaaag gaaatggctc agaaagatct tctttcgttc
      781 atttcacata ccttccggaa tgccctgtcc aacggtcagg aaactgtgcg tcagactctg
      841 tcgcttgcag aaaaggcaat agaaaacgaa tattccgaaa aactttccta taaggcgatc
      901 aataatattg cgggactgta ttcgactttt tccatgattg acagtctgct tgatacattc
      961 agaatgtacg gtctgcacga tgaggaattt atggaagagt ggtcaaaaaa ctttgacggc
     1021 gatacagatg taaactatct ttttgcctat gctctgcgcc agaactgttc caggcttctg
     1081 ttctcagatg agttcaggga ggaacggtac agacttttca atactcaaga ccctgaaatc
     1141 atccggtcaa aaaggaagag ttttcttctg gaagttctgt cactggacct gcagaaggaa
     1201 aatgcagaaa taatactgag ctggtttact gacaatgcgc cttttgttga attaaaaatt
     1261 catgacagca ttacaagttt caatacaaga aaatttcatt ataatttgat tttttccatt
     1321 atcactgaaa cgatgttcaa tgcgttcaaa tattctgacg gaactgttcc ggtaaagatt
     1381 tcctggaaaa gagaaagaaa cagaatagaa tattcctgca aaaatcagtt ctcagcagaa
     1441 agcatcagat actcagacac aaaaagcggt ctgcgtttta ttgagtatct ggtgagaatt
     1501 ctgaaaaatg tagaatttgt ccggacaaga aaaaacagcg tctcagcagc aggtctttcc
     1561 attgaactta tgccctctca gagcagaggc gaagaatgaa gcttctctgg gtggaagatc
     1621 agccgaatga agatcttgca ggaagccttt tcagagaact tctgctcaag acatttgatg
     1681 aagagtttga tcctgacaat gatatcaggg acgaacttcc cggattcttt atttcccctg
     1741 aatacggaat ctctgtctgc ctgagctttc acagttttgt aaatctcttt ttcagattca
     1801 gatttgattt cgatcttttc atcatagaca tcaatctgag cgcaaaggaa ccggtgagtt
     1861 ctgatctgcc tgagggaacc ccagacagac ctgcctttct gaagaaagca ggattttata
     1921 tctggaatct tcttgtgaga aacggcgttc ctgaagacag cattgcattt atgacgggaa
     1981 atacggacag cattcaggaa ttcaatgcaa tgtgcatttc cagcagcatc cccaaactga
     2041 aagatgaaaa cgtattcgac aaaagcgaaa ccgggtatga aaagttcaga aactggatct
     2101 cggaaaagaa gaacagcaga tatattcagc tgagaagagg gatactggaa ggcatacagt
     2161 atcatttaga ttcactcgaa aaaaatactt ctgaaatact ttttaatgta acggtcagaa
     2221 acgacggaac tgcagaacct gtggacacgg actacgcttc gctgtatctc gaaaaactgc
     2281 gcttttattt tcagaaagaa tactcagagg aacctctcag gatgtattca tttctcactg
     2341 ccctttcatc tgaatgggaa cggtcctaca ttcacagcag ctatactgat tcggacaatc
     2401 cgttttcctc aagacatact cctgcagaag aaaagttttt tcattatacc ggattctgga
     2461 tcatgaaaaa tctcagaaat gcaattgccc actcctgtct tgaagacagt cttttggaaa
     2521 gagacattgc attttttttc attatcgcga tgaggacatt ttttaaggct gatctggccg
     2581 cagtttatga ctatgaaaaa attctttttg cggcaatggc agaggagaaa attctgaata
     2641 aaagcataag agagcttcag gaaagatcaa tgaatcatat actgaatctc tgctatcgca
     2701 aacccaaaga gggcagaatg gcagaattca agaaaatcgg gaaccgtttt ctggaatccc
     2761 acaagaaagt cttttcaaga aacaggcatt atgaggaggt tcctgaattc accaaggatc
     2821 tttccaggga cgatattcgg gataattca
//
LOCUS       DAOQGJ010000294         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21539_length_2843_cov_3.485294, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000294 DAOQGJ010000000
VERSION     DAOQGJ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21539_length_2843_cov_3.485294"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="PK683_05925"
     CDS             complement(<1..1312)
                     /locus_tag="PK683_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNH08020.1"
                     /translation="MQMSFKIHSSYKPAGDQISAIQKIAESFGSGNSKVTLVGVTGSG
                     KTFTMAHVIEKLSKPTLVLCHNKTLAAQLFREFKEFFPENAVEYFVSYYDYYQPEAYV
                     PASDTFIEKDSSINEEIDRLRLRATASLLERDDVLIISSVSCIYGLGSPDEYSKSLLM
                     VKKGEMIDREKVIKRLLHIQYNRNDIEFIRGNFRVRGDVIEIMPAYTDEAYRIEFFGD
                     EIESISKFQPVTGKLIAKLDKLAVYPAKHFITNAPSIEAASRKIREEMETQVKKFKSE
                     EKHLEAQRIESRTNYDLEMLKELGYCSGIENYSRHLSGREEGERPACLFDYFPKDFLC
                     IIDESHVTIPQIGGMFAGDRARKETLVNYGFRLPSALDNRPLNFTEFETIVQNVLYVS
                     ATPSEYELKNSAEAVEQIIRPTGLLDPTVEVRPVLNQIEDILNEI"
     gene            complement(1278..2420)
                     /locus_tag="PK683_05930"
     CDS             complement(1278..2420)
                     /locus_tag="PK683_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08021.1"
                     /translation="MPKRKTAVQKSVQGSLSKVTSLVILDQEPIRKKFIKNCQKTIKS
                     FEKAESEWENYKSKDLNEYSQWYEKNFQSLENEVTSLAVEIERLAEMINAVNEVSYFS
                     RMSKGKAYEWVKERKEHPETFSEEEKNEEFNDFFEDSDSFFDDDAREDLQYDGFSSEF
                     IDHFEDYLKKNPKMRFYCEADYSFFENFFQRFHEEYKRNHVPQEERKKKNTAEESLKF
                     IYRNLVRILHPDYRKDEDARADELWHEVQNAYQKKDIERLNTLYALYNIRKGNFRENF
                     TISQIQNVEEEFKVQLKQIRQNIRLAKKQPAWGFSRLKDRKKIHSDLSKKLKMELARF
                     IFQKKQLEKILESYERQPKKTPKKTVPKKKSRQRDYTDEFQDSFFI"
     gene            complement(2413..>2843)
                     /locus_tag="PK683_05935"
     CDS             complement(2413..>2843)
                     /locus_tag="PK683_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH08022.1"
                     /translation="GKTSSGELVSLNQLWNRRHTTKFNGVSYPIQKAAAACYSEQGKK
                     EVQANIKYYMENASIISAELSSAGYEVFGGKNAPYIWLKTPKNLSSWDFFDRLLNEIQ
                     VVGTPGSGFGPSGEGYFRLSAFGKREDVQEAMKRIKTFHA"
BASE COUNT          661 a          633 c          558 g          991 t
ORIGIN      
        1 gaatttcgtt cagtatgtct tctatctgat tcagaacagg acggacttcc actgtaggat
       61 ccagaagacc tgtaggacgg atgatctgct ctacagcttc cgcagaattc ttaagttcat
      121 attcagaagg agtcgctgat acatacagta cattctgaac gattgtttcg aattcagtaa
      181 agttcaaagg tctgttgtcg agtgcacttg gaagacggaa tccgtaattt acaagagtct
      241 cctttcttgc acggtcgccg gcgaacattc ctccgatctg gggaatcgtc acatgcgatt
      301 catctatgat gcagagaaag tctttcggaa aatagtcaaa cagacaggcc ggcctttcgc
      361 cttcctctct tcccgacaga tgtctcgaat agttttctat gcctgaacag tagcctagtt
      421 ctttcagcat ttccagatca tagtttgtcc tggattcgat tctctgtgct tccagatgtt
      481 tctcttccga tttgaatttt tttacctggg tttccatctc ttccctgatt ttccgtgatg
      541 cagcctcaat ggaaggtgca ttcgtaatga aatgttttgc aggatagact gcaagcttgt
      601 ccagttttgc aatcagtttc cctgtaacgg gctggaattt tgaaatggat tctatttcgt
      661 ctccgaagaa ttctattctg taagcttcgt ccgtataggc aggcataatt tcaatgacat
      721 cgcctctcac tctgaagttt ccccggatga attcaatatc atttctgttg tactgaatat
      781 gaagaagtct ttttattacc ttttcccggt cgatcatttc tcctttcttc accataagaa
      841 gagactttga atattcatca ggtgaaccga gaccgtaaat acaggatact gaacttataa
      901 ttaagacatc atctctttcc agaagggatg cggttgcgcg gagtctgagt ctgtctatct
      961 cttcgttgat cgagctgtcc ttttcaatga atgtgtctga agcgggaaca taggcttccg
     1021 gctgataata atcataatag gatacaaaat attccactgc attttccggg aagaattcct
     1081 tgaattcacg gaaaagctgt gcagcaagag tcttgttgtg gcagaggact aatgtcggct
     1141 tgctgagctt ttcaatgaca tgagccattg tgaacgtttt tcccgatccg gtcactccga
     1201 ccagagtgac tttgctgttt ccgctgccga atgattccgc aattttctga attgcagaaa
     1261 tctgatctcc tgccggttta tatgaagaat gaatcttgaa actcatctgt ataatccctc
     1321 tgcctggatt tcttttttgg aacagtcttc ttgggtgttt ttttaggctg tctttcgtaa
     1381 gactccagta ttttctccag ctgttttttc tggaatatga atcttgccag ttccattttc
     1441 agttttttgg aaagatcgga atgaattttt tttctgtcct tcagtctgga aaaaccccag
     1501 gcgggctgct ttttggccag tcggatgttc tgcctgatct gtttcagctg aaccttgaat
     1561 tcttcttcga cattctgtat ctgtgatatg gtgaaatttt ctctgaagtt tcctttcctg
     1621 atattataga gtgcgtacag agtattgagt ctttcaatat cctttttctg gtatgcattc
     1681 tgaacctcat gccagagttc gtccgccctt gcatcctcat cctttctgta gtcaggatga
     1741 agtattctga ccagatttct gtatataaat tttaatgatt cttccgcagt gttctttttc
     1801 ttccgttctt cctgcggtac atggtttctt ttgtattctt catgaaatct ctggaagaag
     1861 ttttcaaaaa aactgtaatc agcctcgcag tagaatctca tcttaggatt tttcttcaga
     1921 tagtcttcaa aatggtcaat gaattcactg ctgaatccgt catactgaag gtcttctctg
     1981 gcatcgtcat cgaagaatga atccgagtct tcaaagaaat cattgaattc ctcgtttttc
     2041 tcctcttcag agaatgtttc cggatgctcc tttctttcct tcacccattc gtaagctttc
     2101 cccttcgaca tccggctgaa ataggaaacc tcattcactg cgttgatcat ttctgcgagt
     2161 ctttcaattt ccactgcaag ggaggtgact tcattttcaa gcgactggaa atttttttca
     2221 taccactgac tgtattcgtt caggtctttg gatttatagt tctcccattc tgattccgct
     2281 ttttcaaaac ttttaattgt cttctggcag ttctttatga attttttcct gatcggttcc
     2341 tgatcaagta tcaccagaga ggtcactttt gaaagcgacc cctgcactga tttttgaact
     2401 gcagtctttc ttttaggcat gaaaggtctt gatcctcttc attgcttcct gcacatcctc
     2461 tctttttcca aatgcactta atctgaaata tccctcgcct gagggaccga accctgagcc
     2521 tggagttccc actacctgaa tctcgttcag aagtctgtcg aagaagtccc aggagctcag
     2581 attcttaggt gttttgagcc agatataagg agcatttttc cctccgaaaa cttcatatcc
     2641 tgcactggaa agttcagcac tgatgatgga tgcattttcc atatagtatt ttatattcgc
     2701 ctgaacttct ttttttccct gctctgaata acatgcagcg gcagctttct gaatagggta
     2761 ggatacaccg ttgaatttag ttgtgtgtct tctgttccag agctggttca gactcaccag
     2821 ttcacctgaa gatgtttttc ctt
//
LOCUS       DAOQGJ010000295         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21544_length_2843_cov_2.409613, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000295 DAOQGJ010000000
VERSION     DAOQGJ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21544_length_2843_cov_2.409613"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..704
                     /locus_tag="PK683_05940"
     CDS             147..704
                     /locus_tag="PK683_05940"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH08023.1"
                     /translation="MPAALSEHPEILEFAVPISLDAYHTLCELYPKLEKTELIQGVIV
                     EKMTKSPEHNFFSNLLIQELQKLKPNASILLVEKPLSLAGSEPEPDLAVVEGTIYDYK
                     FKNPNTAILVVEIAKTSLILDRIKKEIYASADIPNYWIVNLNQKEIEVYSDPVLGNYR
                     TVKNYTSGEKISIFGSELDVSVLFE"
     gene            complement(740..2083)
                     /locus_tag="PK683_05945"
     CDS             complement(740..2083)
                     /locus_tag="PK683_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH08024.1"
                     /translation="MDEKKIGLRIGIIGAGPSGLTLALALCRRENLHITLFEKGPDHR
                     DAPSYNPLRSYTIDITGHGARAVKYLNLNERFSRDLIRFKGIRLPIFRPMIEETYNGE
                     GWTGSRGDIAKAILEELTEQNVSRKNTVIRFGTEAKIADLKKGIISFDEGRQTETFDL
                     IVACDGAGSPARNLIRESDPDFQVAGKDNDNYAMMLPFDRNTEELDSRYLYIFGLPPF
                     LAVAGAINGKNGPSDPLWVCQIGYFGSRKFSSVDEAEDFLRKQYPGRKNNSVLHYAGR
                     KAIEDFAKQENISTGRAKICSAFHSERIVLLGDAAAPFPPVGQGVNAAMEMAVVLDGC
                     LGEQLKKTGMNTEDIVSESIKNFTKKWKPEADAISKISFHGLDLRRFHPVWFGRVKTV
                     GMVILHKIFHRDPMTNAKREDMKYSEALAYQKKTDLILFGVLILAVCTAVYGFIR"
     gene            2359..>2843
                     /locus_tag="PK683_05950"
     CDS             2359..>2843
                     /locus_tag="PK683_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HNH08025.1"
                     /translation="MNLEAQERKEPRIPIPSLNFNVNEAKTPRETSLSLMILFLVTIL
                     SLAPAIIMSMTSFTKIVIVMDFVRRALAIQNLPPNQVMMGLALFMTFFIMAPTLKKVN
                     EQALTPYTDGKIETNEFFDKAMVPIRGFMIRQIGKEGTKDVALFLKLGKVENVSSYDD
                     VP"
BASE COUNT          787 a          631 c          586 g          839 t
ORIGIN      
        1 aattcttcct gttgaaaaaa tatataataa atcattacac tttttctgcc tgttctcatt
       61 gatcgaataa actggctttt tcccactgca ttgggattgc tcattttttg cttgacttct
      121 gaatttcaga gactagcctt tccaatatgc ctgctgcttt atcggaacac cctgaaattt
      181 tggaatttgc agttcctatt tcgctggatg cgtatcatac cttgtgtgag ctgtatccga
      241 aactggaaaa aacagaactg attcaaggag tcatagttga aaagatgacg aaaagtccgg
      301 aacacaattt tttttcaaat ctgcttattc aggaactcca gaagttgaaa ccgaatgctt
      361 caatactgct tgtggaaaaa ccattgtcat tggcaggttc tgaaccggag ccggatttag
      421 ctgttgtgga aggcacaatt tacgactata aattcaagaa tccaaatact gcaatccttg
      481 ttgtggaaat tgcgaaaact tcactgattc tggacagaat caaaaaagag atttatgcat
      541 cagccgatat tccaaattac tggattgtca acctgaacca gaaagaaata gaagtttatt
      601 ccgatcctgt tttgggcaat tatcgaactg taaaaaatta tacttccgga gaaaagattt
      661 caattttcgg atcagaactg gatgtttctg ttttatttga atgagatccg atcactgctt
      721 cagaagaaat ttatgaagat catcttatga atccatagac cgcagtgcag acggcaagga
      781 tcagcacccc gaaaaggatc agatccgttt ttttctgata agccagggct tcggaatatt
      841 tcatatcctc tctctttgca tttgtcatcg gatctctgtg gaaaatttta tgcagtatta
      901 ccattccgac agtcttgact ctgccgaacc agactggatg aaatcttctc agatccagtc
      961 catgaaagct gatttttgag attgcatctg cttcgggttt ccattttttc gtaaaattct
     1021 ttatggattc agaaacgata tcctctgtgt tcattcccgt tttcttcagc tgctctccca
     1081 ggcatccatc caaaacaact gccatttcca ttgcagcatt tactccctga cccacaggcg
     1141 gaaacggtgc agctgcatct cctaaaagca caattctctc agaatgaaat gcggaacaga
     1201 ttttggccct tcctgtggaa atgttttcct gttttgcaaa gtcctcgatt gcctttctgc
     1261 ctgcataatg aagaactgag ttatttttcc tgccgggata ttgttttctg aggaaatctt
     1321 ctgcctcatc aacagagctg aattttctgg aaccgaaata accgatctga cagacccaga
     1381 gaggatcgga tgggccgttc ttgccgttga tagcacctgc cactgcaaga aaaggaggaa
     1441 gaccgaatat atacagatat ctggaatcaa gctcttccgt attcctgtca aagggaagca
     1501 tcattgcata attgtcattg tccttgcctg caacctggaa atcaggatca gattctctga
     1561 tcagatttct tgcaggagaa cccgctccgt cgcaggcgac aatcagatca aaggtttccg
     1621 tctgccttcc ttcatcgaaa gagatgatcc cttttttcag gtccgcaatt tttgcctcag
     1681 tgccgaaacg gatgacagta tttttgcgcg agacattctg ttccgtcagt tcttcaagaa
     1741 ttgcttttgc aatatcgcct ctggacccag tccatccctc tccattgtat gtctcttcaa
     1801 tcattggacg gaaaatcggc agacggattc ccttgaaccg gatcagatcc ctgctgaaac
     1861 gttcattcag attcagatac tttactgccc ttgcgccgtg accggtaatg tcgatcgtat
     1921 aggaacggag aggattgtaa gaaggagcat ctctgtgatc cgggcctttt tcgaacagag
     1981 tgatatgcag gttttctctt ctgcagagtg ccagtgcaag tgtcagtccg gaagggcctg
     2041 ctcctattat gccgatacga agtccgatct ttttctcatc catagctcat tcctgaatcc
     2101 tcacgtcggt cactgcattt ttatgtccgc cgatcctgag aatttgaaag taaaccctgt
     2161 tctgtcaatg tcaagaaaat atctggtctg agatcttctt tcgaaatccc gaatcaaaac
     2221 taattagttg aaaaaaaaac ctgtcatggc aaaaaagcca ataacaaggg tttgcggggg
     2281 agaatgataa gtttattcag aaaaacattg ataggaaaat tggcagtttt tctgctcctt
     2341 ctgcagtttg cgggaaatat gaatctggaa gctcaggaac gaaaggaacc gagaattccc
     2401 atcccaagcc tgaactttaa cgtaaacgaa gccaagacac ccagagaaac cagtctctct
     2461 ctgatgatcc tttttttggt cacaatcctt tctctggctc ccgcaatcat catgtccatg
     2521 acctcattca caaagattgt cattgtgatg gattttgtga gaagggcgct tgcgatacag
     2581 aaccttcccc cgaatcaggt gatgatgggg cttgcgcttt ttatgacatt tttcattatg
     2641 gctcctacgc tgaagaaagt gaatgagcag gcactgactc cgtatacgga cggaaaaata
     2701 gagacgaatg aattcttcga taaggcgatg gttcccattc gcggatttat gatccgtcag
     2761 atcggaaaag aaggcacaaa agacgtcgct ctctttctca agctgggcaa agtcgaaaat
     2821 gtatcgagtt atgacgatgt tcc
//
LOCUS       DAOQGJ010000296         2840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21585_length_2840_cov_3.041293, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000296 DAOQGJ010000000
VERSION     DAOQGJ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21585_length_2840_cov_3.041293"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PK683_05955"
     CDS             <1..356
                     /locus_tag="PK683_05955"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH08026.1"
                     /translation="TFKGTIERISPVFEEKTHTLEIRTVLENRDLRLRPGMFIRIKSN
                     LGKSDIIFKIPRTAVFAAKGGNARVFLKKDGKAFSREIVTSGTEKGNISVISGLSEGE
                     EIVLEPGRVKEGEKI"
     gene            392..>2840
                     /locus_tag="PK683_05960"
     CDS             392..>2840
                     /locus_tag="PK683_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH08027.1"
                     /translation="MAVLLFYISLVILGISSAFRAKVSLLPPADLPKITVRTEFPDSV
                     PEETEALVTVPIMEKLGTVPGISELRAVSESSVSEIYAEFRSRKDLEFQILNVRDRLD
                     SVRTVLPERADKPVILRFSSSDLPFMEISFHSEDVEHLRENILKKWKTPLEKIQGIGK
                     ARVSGGMEKRLRAGLDIQKIGSIGVPAQFLIERMKSSLRNFPSGAVSSGDRDILLRVK
                     GEPQSVEEILDTVLYSVKAALPFRFRDAADLTEEYFKSEEESSVNGKDTVLLELFLET
                     GSNAAEAAAAAEKQLNEILSSEKNVQMRILYNDAEYINGMKKGLLISVFSGALISFLI
                     LLSAYRSLREPLTLFLTLAVSFPVIFLLFETAGIGWNMLSLGGLLLGTGMLLDSSNIS
                     ISSLIRTEKENPSGDYCAVSMDRIFSSLFSSSATTVFVFFPLLFLQNRAGFMLSEMAA
                     SICISVTVSFLLSVTWTPVLYKKLHPFRISFSWPLIERLEKTVFSKYIYILRLFIKNP
                     IQLLKYIAYAALISALCFIFLKKEFLPVLDTKELSLTVNVPSELVQERKDEIRKRTEE
                     IIRNTVDAEFIVLKKKKGSDSFICRILLNRNRFSHTETSILHLENTLREKLEYPFQLK
                     SSGDRLSETVLKYSNKNVFRLLSSDSSALQAAGTSVLSALRKNNDIIYISSVSAVRSP
                     EISLIPDREKLSETGETPESVSSELKTVFSGKEKFRLRLNEEDRAFYADPSQGAVSDI
                     SSLLSYRIGREEFSVPVSSVFRTETSEKPRILTMYGNLHSFEIQAEWKSENARKEQLQ
                     NLRQSLPNTVILQENENE"
BASE COUNT          823 a          559 c          649 g          809 t
ORIGIN      
        1 agacattcaa aggtaccatc gagagaatca gtcccgtgtt tgaagaaaaa actcatactc
       61 tggaaatcag gaccgttctg gaaaacaggg atctcaggct caggccgggg atgtttatac
      121 gcataaaatc taatttagga aaatctgata taattttcaa aattcccaga acagctgtat
      181 ttgcagcgaa aggagggaat gcgagggtat ttctgaaaaa ggacgggaag gcattctcaa
      241 gggagatagt cacatcggga actgaaaagg gaaatatttc cgtgatttca ggtttaagcg
      301 agggggagga aattgttctt gaaccaggcc gtgtgaaaga aggagaaaaa atctgatctt
      361 tcggtttttt gattctgtat acagaaggcc gttggcggtt cttctatttt atatctcact
      421 tgtgatactg ggtatttctt ccgcattcag agcgaaagtg tctcttcttc cgcctgcaga
      481 tcttcccaaa ataactgtgc gtactgaatt tccggattcc gttcctgaag aaactgaggc
      541 ccttgtaaca gttcctatta tggaaaaact tggaactgtt ccgggcattt ctgaacttag
      601 agctgtttca gaaagttccg tttcagaaat ttatgcagaa ttcaggagca gaaaggatct
      661 ggaatttcag atactgaacg tgagggacag gcttgactct gtcaggactg ttctgccgga
      721 aagggcagac aagcctgtaa ttctccgttt ttcctcatct gatctgcctt ttatggaaat
      781 ctcatttcat tctgaagacg ttgagcatct gagagagaat attctgaaaa aatggaaaac
      841 gcctctggaa aaaatacagg gtattggcaa ggcaagagta tccggaggaa tggaaaaaag
      901 gctcagggca ggacttgata ttcagaaaat cggatcaata ggagtacctg cacagtttct
      961 gattgaacgt atgaaaagtt ctctcaggaa ttttccatca ggggcagtca gttcgggaga
     1021 cagggatatt cttctcagag tgaaggggga gcctcagtct gtggaagaaa ttctggacac
     1081 tgttctctat tcagtcaagg cagcacttcc tttccgattc agagatgcag ctgatctgac
     1141 ggaggagtat ttcaaatcag aggaagaaag ctctgtgaat ggaaaagata cagtgcttct
     1201 tgaacttttt ctggaaaccg gaagcaatgc agctgaagct gcagcggcgg cagaaaagca
     1261 gctgaatgaa attctttctt cagagaagaa cgttcaaatg agaatacttt ataatgatgc
     1321 agaatatatc aatggaatga agaaaggtct tttgatttct gtattttcag gagcattgat
     1381 ttcttttctg atccttctct ctgcatacag gtccttgaga gaacctctga cgctgttcct
     1441 cacacttgct gtttcctttc ccgtcatatt ccttctgttt gagactgcag gaatcggatg
     1501 gaatatgctt tcacttggag gtctgcttct tggaacagga atgcttctgg attccagcaa
     1561 tatcagcatt tcctctctga tcagaacaga aaaagaaaac ccctcaggcg attactgtgc
     1621 tgtttcgatg gacaggatat tcagctctct attttcaagc tctgccacta cagtgtttgt
     1681 cttctttcct ctccttttcc tgcagaaccg tgcaggattt atgctttctg agatggcggc
     1741 ttccatatgc atttcagtga ctgtgagttt tctgctttca gtcacttgga ctcctgtcct
     1801 gtataaaaaa ctgcatccgt tcagaatatc cttcagctgg cccctgatag aaagactgga
     1861 aaagactgtc tttagtaagt acatctatat attaagatta tttataaaaa atccgattca
     1921 gcttctgaaa tacattgcct atgcggctct gatctccgct ctgtgcttta tttttctgaa
     1981 gaaagaattt cttccggttc ttgacacaaa ggaactcagt cttactgtga acgttccttc
     2041 tgaacttgtg caggaaagaa aggatgagat acggaaaaga acggaggaga tcattcgaaa
     2101 tacggtcgat gcggagttta tcgtcctgaa aaaaaagaaa ggcagtgaca gcttcatctg
     2161 cagaattctt ctgaacagaa acagattttc ccatacggag acgtccattc ttcatctgga
     2221 gaatactctc agagaaaagc ttgaatatcc ctttcagctg aaaagttccg gggacaggct
     2281 ttccgagaca gttttgaagt attcgaataa aaatgtcttt cggcttctgt cttccgactc
     2341 ttctgctctg caggcagcag ggacttctgt tctctctgca ctccgaaaaa ataatgatat
     2401 tatatatatc agcagtgtca gtgctgtcag atctccggaa atttccttaa tccccgacag
     2461 ggaaaaactt tctgaaacag gagaaacgcc tgaatcagtg tcttccgaac tcaagactgt
     2521 tttttcagga aaggaaaaat tccggctgag actgaacgag gaggacagag cattttatgc
     2581 agatccgtcc caaggtgcag tttcggatat atcatcatta ttaagttatc ggatcggaag
     2641 agaggagttt tcagttcctg tatcctctgt attcaggaca gagacttctg aaaagccgag
     2701 aattctcacg atgtacggta atctgcacag tttcgaaata caggctgaat ggaagtcgga
     2761 aaatgccaga aaggaacagc ttcagaatct taggcagtcg cttccgaata ctgtaatact
     2821 tcaggagaat gaaaatgaat
//
LOCUS       DAOQGJ010000297         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21634_length_2835_cov_3.687410, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000297 DAOQGJ010000000
VERSION     DAOQGJ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21634_length_2835_cov_3.687410"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1267
                     /locus_tag="PK683_05965"
     CDS             224..1267
                     /locus_tag="PK683_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH08028.1"
                     /translation="MAYKLLLCLILAFSIQAETRYVVIGSGKLNVREKPVDGKILFQL
                     NKGDKVQVRAGGEDSEWVSIVSSDGKKGFASPEFLSRIPLDQIEKAEMIGVLQNSDKK
                     DTAVLRVIGLNLGGWKGSSDVLPEYSYLGKKIIQDKEKISLYSESGSGVMLLKEPVKW
                     GCQNINGFKGTVNPQMHSVLSSEKGSVLAYYGTGKISSSGFRAIKEKKMLKKLEASAV
                     KILLSKKISADKIKNLTPSAVQEFEHPFLKKKFISARFSVQKDFMKDKIYTYYIVSSD
                     SPEKILFEHSEEQTDEMAAYGGQYYPVSAYSLDSIFYIVFSNSGFDSHIFSVHSFDGK
                     TLKELMVGAGDAC"
     gene            1269..2288
                     /locus_tag="PK683_05970"
     CDS             1269..2288
                     /locus_tag="PK683_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH08029.1"
                     /translation="MKLHRYEEQAAIEEVGDRIYRAVIPQPFYAPNNIYIIRSDEPAL
                     IDSGYIQNMGILQRALRKIGLSFSRIRHVFYTHNHIDHISGSLTLRAYSSAKHYGMAG
                     MASRIGNYMEYMETFQRAMSRLIYKAMPDPAERKKETEKAGAPWNRLKKAFESSVKVD
                     PILKMDVELVEGDVIDIGGRLLGFMHTPGHNSWHLSPYILGEGIYFTGDLILNNISAV
                     YAELDGNLEHYLRSLERIGKIPVKRILPAHGDEPKDPKRAAKALHKTLTLLERGVIRR
                     LKSGEFDLQDLVLEAMGEKVRNSPYYVTALGIIHAIVLKHTALGNISTVETDPPYEKY
                     LWSGK"
     gene            2285..>2835
                     /locus_tag="PK683_05975"
     CDS             2285..>2835
                     /locus_tag="PK683_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08030.1"
                     /translation="MRILSIYILIFFIGCAGADVKNRDLAEKTAFLKTEDARFELSEK
                     QEESFSTSSDISQFIQEHYMTVSETEVYVHPSRETPSAGKLKFGKKFSVMRVVPEMKS
                     QDPLSQDNGEYFLESVQEDQKFYVRAEDAVPASQCIQKKEGMFCRKAVFSSDKGTLAR
                     ENILLFIRGTEAENLSGLLRLPDS"
BASE COUNT          860 a          553 c          684 g          738 t
ORIGIN      
        1 aatcttgttc tggtcagaaa catttcatca gaaaaggaca gtttcggttt tgctgaactg
       61 ggctgtcctt cctgcggagc cccgcttcct gagtctgatt cagtgaaatg cgggtactgc
      121 aggtcagacc ttccggttca ggtgaatgac tggcttcttg attcagcaat gtgagatttt
      181 agaaaaactt gcatgaaacg tcggggaaaa tttcctatct gatatggcat ataaactttt
      241 actgtgtctg attcttgctt tttccataca ggctgagacc agatatgttg tgatcggttc
      301 aggaaaactg aatgtacggg aaaaacccgt ggatggaaag atactgtttc agctgaacaa
      361 gggcgataaa gttcaggtca gggcaggcgg tgaggattcg gaatgggtga gcattgtttc
      421 gtctgacgga aaaaaaggat ttgcttcccc tgaatttctg agccgcattc ctttggatca
      481 gatagaaaag gcggaaatga tcggagtgct tcagaattct gataaaaagg acactgcagt
      541 tctccgtgtc attggactga acctcggcgg atggaaaggt tcatcggatg ttcttccgga
      601 atattcctac cttggcaaga aaataataca ggataaggaa aaaatatccc tttactcaga
      661 atcgggttcg ggtgtaatgc ttctgaaaga gcctgtgaaa tggggatgcc agaatataaa
      721 cggtttcaag ggaacagtaa atcctcagat gcactcagtt ctttcctccg aaaagggatc
      781 agtgcttgcc tattacggaa ccggaaaaat ttcctcatcg ggtttcaggg caataaagga
      841 aaaaaaaatg ctgaagaaac tggaagcttc tgctgttaaa attcttcttt cgaaaaaaat
      901 atccgcagat aaaatcaaaa acctgacccc ttccgccgtg caggaatttg aacatccctt
      961 tctgaagaaa aaattcatat ccgccagatt ctccgttcag aaggatttta tgaaggataa
     1021 aatatataca tactatatag tgtcatccga cagtcctgag aaaatcctct tcgagcacag
     1081 tgaagagcag actgatgaaa tggctgctta cggaggtcag tactatcctg tgtccgctta
     1141 ttctctggac agcatatttt atattgtgtt cagcaattca ggattcgatt ctcatatatt
     1201 ctctgttcat tccttcgacg gaaaaactct gaaggaactg atggtcggtg caggagacgc
     1261 atgctgaaat gaaactgcac agatatgaag aacaggctgc gattgaggaa gtgggagaca
     1321 ggatttacag ggcggtgata cctcagcctt tttatgcacc caataatatt tacatcatac
     1381 gttcagacga acctgcactg attgattccg gatatataca gaatatggga atccttcaga
     1441 gggcactgag aaagatcggc ttgagctttt cacggatcag acatgtattt tatactcaca
     1501 atcatataga tcatatcagc ggttcactga ctctccgtgc ctacagcagt gcgaagcatt
     1561 acggaatggc agggatggcc tccagaatag gaaactatat ggagtatatg gaaacatttc
     1621 agagggcaat gagcagactg atctacaagg caatgcctga tcctgcggag agaaagaagg
     1681 aaacagaaaa ggcaggagca ccctggaaca gactgaagaa ggcatttgaa agttcagtca
     1741 aggtggatcc tattctgaaa atggatgtgg aacttgtgga aggagatgtc atagacattg
     1801 gaggaaggct tctgggtttt atgcacactc ccggtcacaa cagctggcat ctgagtcctt
     1861 atattctggg ggaaggaata tacttcaccg gggatctgat tctgaacaat atttctgcag
     1921 tgtatgctga actggacgga aacctggaac attatctcag atccctggaa agaatcggaa
     1981 aaatccctgt gaaaagaatt cttcctgctc acggtgatga accgaaagat ccaaagcgtg
     2041 cagccaaggc acttcataag acactcactc ttctggaaag gggagtgatc agaagactga
     2101 agtccggaga atttgatctt caggatcttg tgctggaagc aatgggggaa aaggtcagaa
     2161 acagtcctta ttatgtgact gcgcttggaa tcattcatgc aattgtactc aagcatactg
     2221 ccctgggaaa tatttccact gtggaaactg atcctcctta cgaaaagtac ttatggagcg
     2281 gaaaatgaga atactcagta tatacatttt aatttttttt ataggctgcg caggtgcaga
     2341 tgtaaaaaac agagatctgg ctgaaaaaac agcatttctg aaaacagaag atgccagatt
     2401 tgagctttca gaaaaacagg aagaatcctt cagcacttca tcggacattt ctcagtttat
     2461 tcaggagcat tatatgactg tttccgagac agaggtctat gttcatcctt cgagagaaac
     2521 gccctctgca ggaaagctga agttcggaaa gaagttttcc gttatgagag ttgttcccga
     2581 aatgaaatct caggatcctc tgagtcagga caacggcgag tattttctgg aatccgttca
     2641 ggaagatcag aaattctatg tgcgtgctga ggatgcggtt cctgcatcac agtgcattca
     2701 gaaaaaagaa ggaatgttct gcagaaaggc cgttttcagt tcagacaaag gaacgctcgc
     2761 aagagaaaac attctgcttt tcatcagagg cacggaagct gaaaaccttt cagggctgct
     2821 cagactgcct gattc
//
LOCUS       DAOQGJ010000298         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21638_length_2835_cov_2.637410, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000298 DAOQGJ010000000
VERSION     DAOQGJ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21638_length_2835_cov_2.637410"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="PK683_05980"
     CDS             <1..1389
                     /locus_tag="PK683_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNH08031.1"
                     /translation="LTYAAAIRQEVNPFTYVIYGITGNFPGSFEIQKYKYKIMEYTEI
                     ENRDIWEFRLNLNQEEIDQLTRHLWEMQKTHFDYYFFNENCAFRVLTLLEAARPGLDL
                     TSGSGMFVFPADVIKSTVNQKDLLSAVIFRSSRLESYRKKAEFLEGSETEILKEILNG
                     RLNAGMKLSAERRAVVYDAAADLYAVQNSEDQKDEKRRENYFRTLERLNLNRGNPGSM
                     SSLKGYSERSMDSNPVYAHSPSQAVLSGGSSSSGRFLDIKYRPVLREFTDPYAGYSLY
                     NQLFFLNTNLRYYEDIRSFRIHEIHFIQMNSLHPAGNLLYRPSWRFDSGVQSLTPEIK
                     SGKNSDRVYGFLNAGLGYSWEPFSGIWRRNLLFYSFLDFRMEESGSFKEHYRAGTKST
                     LGILMRFTDTFSAHLYFDYRSYFSGQKGFMPELTFASNLLLTSSLALEIFYKKNYYSG
                     WEEELIGLKYYF"
     gene            1498..2709
                     /locus_tag="PK683_05985"
     CDS             1498..2709
                     /locus_tag="PK683_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HNH08032.1"
                     /translation="MKTLPVLILMIFLSFCKKEVSIQADSEFQKKTTEALLKAKPNSV
                     ISLPSGKFVLDSSLSLTVSGVTLRGAGMDKTVLSFKGQKNGAEGILVRGVKGFTIEDL
                     TIQDTKGDAVKVTESSDVIFRRVKTEWTDGPKETNGAYGIYPVQCKNVLIEDSTAIGA
                     SDSGIYVGQSSNIIVRRNRAEFNVAGIEIENSVYADVYENTATKNTGGILVFDLPGLP
                     VQGGKNTRVFKNKVYDNDTENFAPAGNIVAKVPTGTGIMVMANDNIEIFDNDISKHPT
                     VNMTIVSYYITDIPIKDPKYDPYPEGIHIHHNRFLEGGYSPKGLIVKALSFKIGTPFP
                     DILYDGIKDSKKMKDGKLPETSRLCIHDNGNVSFADIDAENTFRNINRNLKDFECSLN
                     ALEEVKLSFLK"
BASE COUNT          878 a          533 c          633 g          791 t
ORIGIN      
        1 ctcacgtatg ccgcagcaat aaggcaggaa gtaaatccgt ttacttacgt aatttacggg
       61 ataacaggaa attttcccgg ctcttttgaa atccagaaat ataaatataa aataatggaa
      121 tatactgaaa ttgaaaacag ggatatctgg gaattcaggc tgaatctgaa tcaggaggaa
      181 atcgatcagc tgacaagaca tctttgggag atgcagaaga cacatttcga ttattacttt
      241 ttcaatgaga actgtgcttt cagggttctg actcttctgg aagcagcacg tcccggtctt
      301 gatcttacat ccggttcagg aatgtttgta tttcctgcag atgtcattaa atccacagtg
      361 aatcagaaag atctgctgag tgctgtcatt ttcagaagtt caagactgga aagctacaga
      421 aagaaggcag agtttctgga aggaagtgaa actgaaatcc tgaaggaaat attgaacgga
      481 cgcctcaatg caggaatgaa actttctgca gagagaaggg ctgtcgtata tgatgctgca
      541 gctgatcttt atgcggtgca gaattctgaa gatcagaaag atgaaaaaag aagggaaaac
      601 tatttcagga cacttgagag actgaatttg aatcggggca atccaggttc aatgagcagt
      661 ctgaaaggat acagtgaaag gtcaatggat tcaaacccgg tctatgcaca ttctccttct
      721 caggcagttc tttccggcgg cagttcttct tccggaagat ttctggatat aaaatacaga
      781 cctgttctga gagaatttac tgatccatat gcgggatact ccttatataa tcagctgttc
      841 ttcctgaata caaatttacg atactatgaa gacatacgtt ctttccgaat acatgaaatc
      901 catttcatac agatgaattc actgcatcct gcagggaatc tgctttacag accatcctgg
      961 agatttgatt ccggagttca gtccttgact ccggaaatca aatcagggaa aaactctgac
     1021 agggtttacg gatttctgaa tgcaggactg ggatattcct gggaaccgtt cagcggaata
     1081 tggagaagaa atcttctttt ttacagtttt cttgattttc gtatggaaga gtcaggaagc
     1141 ttcaaagagc attacagagc aggaacaaaa tcaactctgg gcattctgat gcggtttact
     1201 gatacttttt ccgcgcatct gtactttgat taccgctcct atttttcagg ccagaaagga
     1261 tttatgccgg aactgacatt tgcatccaac ctccttctga catcgagtct tgcactggaa
     1321 atcttttata aaaaaaatta ttattcaggc tgggaagagg aattgatcgg tctcaaatac
     1381 tatttctgaa tactgttctt tgccggaaag gaatcagtca aaaattcggc aggcagagac
     1441 tttgtgccca gatttttcca ttgcactaat tcagtgcttc ctgtaaaaag gttttctatg
     1501 aaaactcttc cggtattaat tttaatgata tttctgtctt tctgcaaaaa agaagtttcc
     1561 attcaggcgg attctgaatt ccagaaaaag acaacagagg cacttctgaa agcaaagccc
     1621 aattctgtaa tttctcttcc atcggggaaa tttgtgctcg attcttctct gtcgcttact
     1681 gtaagcgggg tcactctcag gggggcaggg atggacaaga ctgtgctttc gttcaaagga
     1741 cagaagaacg gtgcagaagg aatcttagtc agaggtgtaa agggatttac gattgaggat
     1801 cttacgattc aggacacaaa aggcgacgcg gtaaaagtga ctgagtcgag cgacgtgatt
     1861 ttcagaagag tcaagaccga atggactgat ggaccgaaag agacaaacgg ggcttacgga
     1921 atttatcctg ttcagtgcaa aaatgtcctg attgaagaca gcactgcaat aggagcttct
     1981 gattcaggga tctatgtcgg acagtcttcc aatatcattg tacggagaaa ccgtgcagag
     2041 ttcaacgtgg cagggattga aattgagaat tctgtgtatg cagacgtata tgaaaacact
     2101 gcgacgaaaa acaccggggg gatccttgtg tttgatcttc caggacttcc tgtgcagggc
     2161 ggcaaaaata caagagtgtt caaaaacaag gtttatgaca acgacactga aaattttgca
     2221 cctgcaggaa atattgtagc gaaggttccg accggcacgg ggattatggt catggcaaac
     2281 gacaatattg agatatttga taatgatatt tcaaaacacc ctactgtaaa tatgacgata
     2341 gtcagttatt atattaccga tattcctatc aaggacccta agtacgatcc ttatccggaa
     2401 ggaattcata ttcatcacaa cagattttta gaaggaggtt attctcccaa gggactgatt
     2461 gtaaaagccc tttccttcaa gattggaact ccttttccgg atattcttta tgacggaatc
     2521 aaggattcca aaaaaatgaa agacgggaaa cttccggaaa cgtccagact ctgtatccac
     2581 gacaacggaa atgtatcatt tgcagacatt gatgcagaaa atacattcag gaacatcaac
     2641 agaaacctga aggactttga atgcagtctg aatgcactcg aagaggtgaa gctgagtttt
     2701 ttaaaatgac aactgaatct tttttttcat cgtattccca aatctcaaca tcgggcggta
     2761 tgagatgaac gtatcggctg agataatact cagccaatgt ttgtttcttt ttttcgttga
     2821 agttttttaa aacga
//
LOCUS       DAOQGJ010000299         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21736_length_2826_cov_3.707687, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000299 DAOQGJ010000000
VERSION     DAOQGJ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21736_length_2826_cov_3.707687"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PK683_05990"
     CDS             complement(<1..234)
                     /locus_tag="PK683_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNH08033.1"
                     /translation="MTQHSVFQITQKLFLRKGQKLLVLRDRKSGLGDLPGGRMTEKEF
                     FSDWKESLKRELVEELGSSFSIKIHDDIILVHRH"
     gene            complement(231..2678)
                     /locus_tag="PK683_05995"
     CDS             complement(231..2678)
                     /locus_tag="PK683_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08034.1"
                     /translation="MADSLDTSLGYPVIKNGTVWEIDLSHLRIFTGLSVLARIIGDTV
                     IDAVENDSGDISFLYKINPNINPELLVMQVYYIQIYAKSGVLDNVLLFKEEFHEHLRA
                     VFGTFQRPVWGSQIHPEFYGEDSKTSPPRALVFPFHYVSQSDDIDYHFILERAENRKH
                     RGRFFFRLTIEKQSEATYQYENRPYSIVDDLRTRVFIAGSTKMAESLSEKLQSACKKG
                     SDSYSEENLVLGHVFEQLSKTGIGAVDQINFQWDKEFSKFMLNTGKEDRLVIIKRLFL
                     CLEDNVLCDLLLKGETVMIRLGRFSIYLFLTRLSRVLNFSINRERNYLTLDMYLKRMP
                     KLETMADKTERDLDFSGYRIFLIHHITSEILALLDAFSRLKTEEINVIFVKYSGLVPP
                     AYLDVLLEIPDPTFKAFGLVRNMTSSGKEYFTLSHAYSEINDLRPVAVWLEEKKVNFF
                     EAMKFLSLHCFLKFALKARQENAKVLLIEDGGYLAPLINELSFKNTGISDVFSEYLLN
                     VLPQEDLSFSSFCADVLVGTVEHTRNGYDRMMKVKGIYGKLFLPAYSIAISNSKVVEE
                     SKEVAHSILSAVESILHGQGMVLSQRKIAVLGSAGNIGKFLCEYLLSGRLHDANKAIV
                     RVDLKNSVSTAKKEYNYLSEIPEDDFLSLEMFLGVIGESILKKEFIERLILDGKSRRL
                     LFASGSTKTVEFSDLNEFLYALAEMEHPAIAGIPVKIHFERIQDPQSRIDQGGKAVIH
                     FERAGKTVEKVLYLLGDLSPINFLFYGVPTETMDMIIAGLTSVSLGLVDQHRNGRLPK
                     PDLYAVDHEIDMWGEKV"
BASE COUNT          801 a          651 c          518 g          856 t
ORIGIN      
        1 gtgtctgtga acaagaatga tatcatcatg aatttttata ctgaaggaac ttcccagctc
       61 ttcaacgagt tctcttttga ggctttcttt ccagtcgctg aaaaactctt tttctgtcat
      121 tcttcctccg ggcaggtcac ccaatccgga tttcctgtcc cttagaacaa ggagtttttg
      181 accctttctg agaaagagtt tttgagtaat ctgaaataca gaatgctgtg tcaaactttt
      241 tctccccaca tatcaatctc atgatcaact gcgtacagat caggcttagg cagtctgccg
      301 tttctgtgct gatcgacaag tcccagactc acgcttgtca ggccggcgat aatcatatcc
      361 atcgtttctg taggcactcc gtaaaaaagg aaattgatcg gactcagatc accgagcaga
      421 taaagtacct tttccactgt cttcccggct ctttcaaaat gaatcactgc ctttccgccc
      481 tgatcaatcc ttgactgcgg atcctgaatc ctttcaaaat gaatcttgac aggtattcct
      541 gctatcgcag gatgctccat ttcagccaat gcatacagaa actcattcaa atcactgaat
      601 tcgaccgtct tggtagaacc ggaggcaaag agaagccttc tggatttccc atcgagtatc
      661 agtctttcaa tgaactcttt tttcagtata gattccccga tcactcctaa aaacatttcc
      721 agtgacagaa aatcatcttc aggaatttcc gaaagataat tgtattcttt ctttgcagtg
      781 ctcactgaat tcttcagatc cactcggaca attgccttat tagcatcatg aagtcttccg
      841 ctcagaagat attcgcagag aaacttgcct atatttcctg cactgcctaa tactgctatc
      901 tttctctgag aaagcaccat cccctgtcca tgcagaatgg actccactgc actgagtatg
      961 gaatgagcca cttccttgga ctcttctaca accttgctgt tggatattgc aattgaatag
     1021 gccggaagaa aaagttttcc gtaaattccc tttaccttca tcattctgtc atacccgttc
     1081 ctggtgtgtt caacagttcc gacaagtaca tccgcacaga aagaagagaa gcttaaatcc
     1141 tcctgaggca gaacattcag aagatactct gagaaaacat ctgaaatacc ggtattttta
     1201 aaagaaagtt cattaatgag gggcgccaga tatcctccat cctcaatgag aagaactttt
     1261 gcattttcct gccttgcctt cagtgcaaat ttcaggaagc agtggagtga gaggaatttc
     1321 attgcttcaa aaaaattgac cttcttctct tccagccaga cagcaactgg acgcaggtca
     1381 ttgatttcac tgtatgcatg agacagagta aaatattcct ttcctgaaga agtcatattt
     1441 ctgacaagac cgaatgcctt gaatgtcgga tcaggtattt ccagaagcac gtccagatag
     1501 gcaggcggaa ccagaccgct gtattttaca aagatgacat tgatctcttc cgtcttaagt
     1561 cggctgaatg catccaggag tgcgagaatt tcggatgtaa tatgatgaat taaaaatatc
     1621 ctgtatcccg aaaaatccag atccctttct gtcttgtctg ccatagtttc aagcttaggc
     1681 attcttttca gatacatatc gagagtaaga taatttcttt ctctgttgat cgaaaagttt
     1741 agaactctgc tgagtctggt caggaaaaga taaatgctga atcttcccag acggatcatt
     1801 acagtctcac ccttcagaag caggtcgcag agcacattgt cttcaaggca gagaaacagt
     1861 cttttgatga tgaccagcct gtcttccttt ccggtattca gcataaattt ggaaaattcc
     1921 ttgtcccact gaaaattaat ctgatccacc gcaccgattc ctgtcttgga gagctgttca
     1981 aatacatgac ccaatacaag attttcctct gaataagaat ctgatccctt tttacaggca
     2041 ctctgcagtt tttcactgag agattctgcc atctttgtac tgcctgcaat aaatacacgt
     2101 gttctcagat catctacaat cgaataaggc ctgttttcat actgataagt tgcttcagac
     2161 tgtttttcaa ttgtcagacg gaagaaaaaa cgtcctctgt gttttctgtt ttcagctctt
     2221 tccagaataa aatgataatc tatatcgtca ctctgactta cataatgaaa aggaaataca
     2281 agtgctctag gaggcgaggt tttggaatct tctccgtaaa attccggatg aatctggctt
     2341 ccccagactg gtctctggaa ggtgccgaat accgccctta aatgctcgtg aaattcttcc
     2401 ttaaaaagaa gaacattgtc aagaactccg ctttttgcat aaatctgtat atagtatacc
     2461 tgcattacaa gaagctcagg attgatattg ggattaatct tgtaaagaaa tgaaatgtcc
     2521 cctgaatcat tttccactgc atcaattact gtatccccga taattcttgc aagaaccgaa
     2581 agtcctgtaa aaatcctgag atgagaaaga tctatctccc agacagtacc atttttaatt
     2641 acaggatatc ctaacgaagt gtctaaagaa tcagccattc tgcctcacta ttacttttac
     2701 agatgaactt tcttttgcct gggcaaatgc tttttcaatt tcaccggatg agaactcatg
     2761 ggtgatcatt ctttctctca ttttttcagc cagaacaggg ttctccgaca taagtttcag
     2821 tgccag
//
LOCUS       DAOQGJ010000300         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21750_length_2825_cov_3.377617, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000300 DAOQGJ010000000
VERSION     DAOQGJ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21750_length_2825_cov_3.377617"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PK683_06000"
     CDS             complement(<1..487)
                     /locus_tag="PK683_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08035.1"
                     /translation="MSSRTALNFLFILYAIVFNSCRLNELVYEPKTTPEQWCRIRPCI
                     DMGGFIFNEPLGSFLVFLLAFLWVLSGFYFLKKNSGQKSRNWFGIALVLGGIGAFQAG
                     ISYQAFSYLLKCAGREFCQLTNGFEVGYSVTQAVSVSAMMTAMAFACTKGLFRKSIIV
                     YS"
     gene            1041..2105
                     /locus_tag="PK683_06005"
     CDS             1041..2105
                     /locus_tag="PK683_06005"
                     /inference="COORDINATES: protein motif:HMM:NF016907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNH08036.1"
                     /translation="MTIQDELQRILEKLKEDENMKVRVALFGQPGSGKSSIINRLIGK
                     KVAPTGQKTDTTLAEKPYEWNGLLLVDLPGYGTAQFPKETYFDEFELDSFDLFLCVFS
                     GKIMQADHDFFQDLKKSGKLCIFVRNKRDEIWEEGRDITELEAEIVSDVRKQIQSEDN
                     EIIFTSCRDSYGFDVLQQKIYENLEPAKKIKWAKGVKAYSAQFLEEKKDACSKYVYIA
                     AGSAAANALNPIPGADIAVDAAVLVSLFASIRTSYGLTEEKIKAVEVMMPAVAPLAYN
                     IIHHATKKGIMLLLKKFIGRQTLKQISKYIPFVGQAIAASAGFGITLAAGMAYLDDCH
                     RLSEELLKKEILSPDQKKLE"
     gene            2354..2716
                     /locus_tag="PK683_06010"
     CDS             2354..2716
                     /locus_tag="PK683_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08037.1"
                     /translation="MQNREIKKEIVFSSTVFLFTFLFFWSLSVDRTKKNSSSHSKLVV
                     VFRKDISSDAAAQIMKKTGNFFSSGPDSVPEKLLNETGPKYIIQVSNDQKSAELSRLK
                     RLNEVYEVYESDLNFQND"
BASE COUNT          857 a          546 c          610 g          812 t
ORIGIN      
        1 gactgtagac aatgatgctt tttcggaaaa gtccttttgt acaggcgaat gccattgccg
       61 tcatcattgc gcttacgctt actgcctggg tcacgctgta tccgacttca aaaccgtttg
      121 tcagctggca gaattccctt cccgcgcatt tcagaagata actgaatgcc tgatagctga
      181 ttcctgcctg aaatgctcct attcctccca ggacgagtgc aatcccgaac cagtttctgg
      241 atttctgtcc gctgtttttc ttcagaaagt agaagcccga aagcacccag agaaatgcca
      301 gcagaaagac cagaaatgaa cccagcggtt cattgaagat aaatcctccc atatcaatgc
      361 aagggcgtat tctgcaccac tgttcaggag ttgtcttagg ttcatagaca agttcattca
      421 gtctgcagga attaaataca attgcatata atataaaaag aaaattcagt gctgttctgc
      481 tgctcatttt ttttatcctt ggaggtttat cattgttcat accggataat gaagtcaagc
      541 tgtttttttc cttctgtcta aattgactct gacattgttc aaaaaaaaaa tttccagtca
      601 gaatctgaaa agaagctgat tttgggatgg gagtttgcag ccgtttccat acattaggac
      661 tgtcaaaatg ttcctgcaat cacagtcaaa aaaaacgcag acagccaaaa cggtgcaaga
      721 gaatgggtaa aaaaatttta actactcagc gtattttttc taaaactcaa taaaatcaag
      781 gtttgtaaga aaatgttaat gcttttgaaa ctgaaattaa tgaaaaagca gggattttta
      841 gccgcgtccc agctttgcga gggacgacag tcccttagca gcttccgcac ttgcgaaacc
      901 gccggtaggc atactcccca agcaaactgt ggggttaaag taactacgac gcaattgcgt
      961 gagccgctgc cgcaaggctg agtagttgcc aaaaaattta cattcttgat ttttcctgca
     1021 ggcaaaatta aatgtgccgc atgacaattc aggatgaact tcagagaata cttgagaaac
     1081 tgaaagaaga tgaaaatatg aaggttcgcg tggcgctttt cggtcagcct ggttcaggca
     1141 agtcttcaat catcaacagg ctgatcggaa aaaaggtcgc accgaccggt cagaagactg
     1201 acaccactct tgcggaaaaa ccgtatgaat ggaacggtct gcttctcgta gatcttccag
     1261 ggtatggaac agcacagttt cctaaagaga cttattttga tgaattcgag ctggattcct
     1321 ttgacctttt tttatgcgta ttttcaggca agattatgca ggccgatcat gatttttttc
     1381 aggatctgaa aaagagcgga aagctttgta tttttgtaag aaacaaaaga gatgagatct
     1441 gggaagaggg cagggatatc actgaactgg aagctgagat tgtttcagat gtgagaaagc
     1501 agattcagtc agaggataat gaaattatat ttacgagctg cagggacagt tacgggtttg
     1561 atgtgcttca gcagaaaatt tatgaaaacc ttgaaccggc aaaaaaaatt aaatgggcaa
     1621 agggggtgaa agcatattcc gcccagtttc tggaagagaa aaaggatgcc tgttcaaaat
     1681 acgtttacat tgcagcagga agcgctgctg caaatgcatt gaacccgata cccggagcag
     1741 acattgcagt ggatgctgca gttcttgttt cactgtttgc ttccattcgg acttcgtacg
     1801 gactgactga agaaaagatc aaggctgtgg aggtcatgat gcctgcggtt gctccgctgg
     1861 catataatat cattcatcat gcaacgaaga aagggattat gcttcttctg aaaaaattca
     1921 taggaagaca gactctgaaa cagatatcca aatatattcc tttcgtagga caggcaatcg
     1981 ccgcctcagc cggattcgga atcactctcg ccgcaggaat ggcatatctg gatgactgtc
     2041 ataggctttc agaagaactg ctgaaaaagg aaattctttc tcccgatcag aagaaactcg
     2101 aatgattctc atattataaa tgtattatgt ttaaatttta attaaattag aatatttaga
     2161 ccggtcttta ttcatctcag ctgccttttt tcggattctc aaattgaaac gattccctta
     2221 taatcccctt ttttttagat ttgccttctg cttttcatca gagagagtag atctgtgtcg
     2281 gaggcgcttc tttacggatt acggctgcag gacagtctat tcatgaaccg gagcttcttg
     2341 aaggagacag aagatgcaga acagagaaat taaaaaggag attgtatttt cttcgacagt
     2401 ctttcttttt acatttcttt ttttctggag tctgtctgta gacagaacga agaaaaactc
     2461 ttcatcacat tcgaaacttg tcgtagtgtt cagaaaggac atcagcagcg atgctgctgc
     2521 ccagattatg aaaaaaacgg gaaatttttt cagttcagga ccagattcag ttcctgaaaa
     2581 attattaaat gagacaggtc caaaatacat tatacaggtc agtaacgatc agaagtcggc
     2641 agaattgtcc agattgaaga gacttaatga ggtgtatgaa gtttatgaat ctgatttgaa
     2701 ttttcagaat gactgaatcc aaatctcaat gaaatcattg tttttatata ttatatgtat
     2761 gcttattttt attcgatgtt catccgtgac tgcctactgg tactccgaac cggaagataa
     2821 gtttt
//
LOCUS       DAOQGJ010000301         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21751_length_2825_cov_2.949819, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000301 DAOQGJ010000000
VERSION     DAOQGJ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21751_length_2825_cov_2.949819"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PK683_06015"
     CDS             complement(<1..441)
                     /locus_tag="PK683_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNH08038.1"
                     /translation="MQNLSRTELTRYSRNILLPEIGKEGQLKLKNARVSVIGAGGLGS
                     PVLLYLAAAGIGHLTVIDSDTVDLTNLQRQVLFQTDSVGRKKASEAEGRIHQINPHLN
                     LSVFDRRLTPETVSDMLSPSDLVIEGSDNFMTKFLVNDFCFHTDK"
     gene            complement(444..785)
                     /locus_tag="PK683_06020"
     CDS             complement(444..785)
                     /locus_tag="PK683_06020"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNH08039.1"
                     /translation="MYITELLNENIKALCDVKKNPASIKFGFCKSQLKNILGNAGIDY
                     MHFQDLGIESYRRKNLKGQMDYKKLFTEYEKITLPIVKAHLLKLSDVCRKSRRAALTP
                     FLPNEFSVEHL"
     gene            1348..1527
                     /locus_tag="PK683_06025"
     CDS             1348..1527
                     /locus_tag="PK683_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08040.1"
                     /translation="MRKSILIFTAVILLTSSNCRSREVKEIESDDILEEELRAEPEPF
                     RETRKRDLKNPSKKQ"
     gene            1530..>2825
                     /locus_tag="PK683_06030"
     CDS             1530..>2825
                     /locus_tag="PK683_06030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNH08041.1"
                     /translation="MYTFLLFFLSVLPLPISYFLYRRYDIDRQDIGESIDAFLYGVIS
                     AAALVVLFPYISGFFSQDNEFLRAFLQASLIEKALAYLALLFLLRRSRDKLSVTEAMS
                     LGIFCGLGFAGMENLFYAYEIRKFDVILRLFSSVPLHITTCAIMAYFLGQFYLTVTMS
                     EKIIHLIRAAVIPILFHTFYDLALLRGDNSTYYIAPVLVILISVQEYLMAKSKTLPGA
                     KELKNRKLGLEDFFIIDKQLEYERWILLSMGKKNVEKVPFFSFRVTKTRQFYIILLTL
                     FGFSYFLYDDFLNETLMMSLRPEEQITLFFLYPLVGAFNLILAKSINPEYFRSSRLSL
                     PVVADVTLKSKKETLELNGSDLNSFCVFVKTLDFMDIGEKKEITFKYSDKISPVLPAE
                     VVWDNHENLFEPIGTLFHIRNSSLEFRKFLIKFNFFRFVR"
BASE COUNT          794 a          617 c          529 g          885 t
ORIGIN      
        1 tttgtccgta tgaaagcaga aatcattgac cagaaatttc gtcataaaat tatcagaacc
       61 ttcaatcacg agatcggaag gggaaagcat atccgaaaca gtctccggtg tcagtctcct
      121 gtcaaaaaca ctgagattca gatggggatt gatctgatga atacggccct ctgcctccga
      181 tgcttttttc ctgccgacag aatctgtctg aaaaagcacc tgcctttgca gattggtcag
      241 atccactgta tcggaatcga tgactgtcag atgccctata cctgcggcag caaggtagag
      301 aaggacaggc gaacccagtc ctcctgcccc gatgacgctc actctggcat tcttcagttt
      361 cagctgacct tcctttccga tttccggaag caaaatattt cgggaatacc tggtgagttc
      421 tgttctggaa aggttttgca taatcacaga tgctccacgg aaaattcatt tggcagaaaa
      481 ggagtcaatg cggctcttct gctttttctg cagacatccg acaattttaa taaatgtgcc
      541 tttactatag gaagggtaat cttttcatac tctgtaaaca gttttttgta atccatctgc
      601 ccttttaaat tttttctcct gtatgattct atgccaaggt cctggaaatg catataatct
      661 attcctgcat ttcctaaaat attttttaac tgacttttgc aaaaaccaaa tttgatgctt
      721 gcaggatttt tctttacatc gcacagagct tttatatttt cattcaataa ttcagtgata
      781 tacatttcaa cggtcatcag ttcatatcct tgaactcaga aaaaaaatcc tatcaggcaa
      841 tctcagtgtc tccataagtc tttttgatcc atactgctga atttaccgga atgaccccat
      901 acttgatcat agtctttata tcctgatcag cctgtgcaga aatacatccg gaacggaaaa
      961 tgtgccgccc aaaatctgaa acagagcaaa tatgattttt ctcccgtaat acatagactt
     1021 tggaattttt ttcattctca attctcattc tcctcttcag atttttctct gttcaaaatg
     1081 cggtctatgt ccatccaaat gaaccggcct cagggtaatt atagtaaatg tcggcaggaa
     1141 acggaataag tcctttgaga tttgtcctga aatcatccct tatttgcggc actaaactgc
     1201 atctgctgaa agagccgctg tgaaaaagac gctgttcagg tattcaaaaa catcgacccc
     1261 gcctctgagg cttcgttttt actccaaatt cagaaatcga gcaaattttc cgcttgcgaa
     1321 aaataaaact ttgataatac tttcgtcatg agaaagtcca tcctgatttt tacagctgtc
     1381 attctgctga catcctccaa ctgccgatcc agagaagtca aagaaattga gtctgatgat
     1441 attctcgagg aggaactgcg tgcagaaccg gaaccgtttc gggaaacaag aaaaagagac
     1501 ttaaaaaatc cctcgaagaa gcagtaatct tgtacacttt tctccttttt tttctgtctg
     1561 ttctgcctct tccaatatcc tattttcttt acagaagata tgatattgac agacaggata
     1621 taggagaatc gatagacgca tttctttacg gtgtcatctc agcggcggca ctcgtagtcc
     1681 tttttccata tatttcaggc tttttttctc aggacaatga atttcttcgg gcatttctgc
     1741 aggcttcact gatagaaaaa gctctggcat accttgcact tctcttcctt ctcagaagaa
     1801 gcagagacaa actttcggtg accgaggcta tgtctctcgg catattctgc ggactgggat
     1861 ttgccggaat ggaaaatctg ttctatgcct atgagattcg gaaatttgat gtcattctca
     1921 ggcttttcag ctcagtccct cttcatatca caacctgtgc aattatggct tattttctcg
     1981 gacagtttta tcttacagta actatgtccg aaaaaatcat tcatctgatt cgggccgctg
     2041 tcattccgat tctgttccat accttctatg atcttgcact tctccgaggt gataacagca
     2101 cttattatat tgcgccggtt ctggtaatcc tgatttccgt tcaggaatat ctgatggcca
     2161 aaagcaagac ccttcccggt gcaaaggaac tgaaaaacag aaaactggga ctggaagatt
     2221 ttttcatcat cgacaagcag ctggaatatg aaagatggat acttctctct atgggcaaaa
     2281 agaatgtgga aaaggttcct ttcttcagtt ttcgcgtcac aaagactagg cagttttata
     2341 tcatacttct gacccttttc ggattcagtt attttctcta tgatgatttt ctgaatgaaa
     2401 ccttaatgat gagcttaagg ccggaagagc agattactct attctttctt tatcctctgg
     2461 taggcgcttt caatctgatt ctggcaaaat caatcaatcc ggaatatttc agaagcagca
     2521 gactgagtct tcctgtcgtt gctgatgtaa cactgaagtc caaaaaagaa acccttgagc
     2581 tgaacggttc tgatctgaat tcattctgcg tctttgtaaa gacactggat tttatggaca
     2641 taggagaaaa aaaggaaatc actttcaaat actcggacaa gatatcacct gtgcttcctg
     2701 ctgaagttgt ctgggacaat catgaaaatc tgtttgaacc gatcggaact ctttttcaca
     2761 tcagaaacag ttccctggaa ttcagaaaat ttcttataaa attcaatttt ttccgatttg
     2821 taaga
//
LOCUS       DAOQGJ010000302         2821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21803_length_2821_cov_2.742227, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000302 DAOQGJ010000000
VERSION     DAOQGJ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21803_length_2821_cov_2.742227"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..2122)
                     /locus_tag="PK683_06035"
     CDS             complement(194..2122)
                     /locus_tag="PK683_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH08042.1"
                     /translation="MIQLDSMPPKFTLYQLIKSAADSHPNAPAQMYRESSTSDFRSKT
                     FSEMFTEIKKISLALNSLGMKKGENVGLIADVGHRWMPVSMGITTIGGVDVPRGTDAT
                     QGDLSYIFQHADCRIIFLETVKVYKKIESILSQFKNLKYIVFFEDFSSLKIPSSVQAM
                     TLSELMNKGEALLKNGDGEYHKLAEAVSEPDTATIIYTSGTTGAPKGVVHTQKSLTWE
                     VYYSLKGMDVPPLGVTMGFLPPWHIAERLIEMACIRIVSAVAFTSVPSLAKDLQAVNP
                     TFLLSVPRVWESFYNKMMGSVAKASPVAKALFNFANWTALKFSTLKDILLGLKYSLEP
                     DSIIVSGAKKIGALIGCILLFIPNILAQAILKKVKGALGNRIKFAISGAGALPEYIDR
                     LFYSIGVPIIETYGMTETVGVSVRRKMPGTVVGTVGSPIEGVEIKLVDSQGNTVTQPN
                     VKGVAYHRGPHIMKEYYKEPEKTKQVLTEDGWLNSGDILVWTAGGQLKFAGRAKDTIV
                     LLGGENIEPLPIEDSIKQSAFVAQCVVVGQDQKTLGALILPDKDAVITAMKEKNITLS
                     ADMTEWNKIPEVTALFKEEVKKFNSSEMGFKSFEKVTAFHLLGKEFTVDDELTQTLKI
                     KRNVVADKYSKEIENMFK"
     gene            2247..2783
                     /locus_tag="PK683_06040"
     CDS             2247..2783
                     /locus_tag="PK683_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08043.1"
                     /translation="MPNISETAGRSQIANGKSCNFFLDSLLHLCNLYFLNGSEADMGA
                     WGVGSDENDDTFNFLGDFYLPQADRGSKNPAEIYDVPAIYKFLKETELLYEYISDVGM
                     VVYFAKNKKAFPNLPKKAVKTIIGFIDQELAELTSDPLVTTRTWDPADYRERISKLKK
                     EKKILENYLKPKTSKKKK"
BASE COUNT          758 a          599 c          593 g          871 t
ORIGIN      
        1 gtcattgaaa ttcctgtatt ctttcactgc tggggtattg gaccttggaa tttggaaaga
       61 tcataacccg gtctctattg taaactgcat aaaagcaggc cctgaaaaaa ttctttgcag
      121 aatctgcaca atgaaaaatg gatataatat atccactccg gaaatcctct tccacagtgc
      181 cccagaaaga cactcatttg aacatatttt ctatttcttt tgagtatttg tctgctacta
      241 catttctctt tattttcaat gtctgcgtaa gttcatcatc cacagtgaat tcttttccca
      301 gcagatgaaa tgctgtcact ttttcgaaag acttgaaacc catctcactt gagttgaatt
      361 tcttaacctc ttccttgaac agggcagtga cctcaggaat cttgttccat tcagtcatat
      421 ctgcgcttag agtaatgttt ttttctttca tggctgtaat gacggcatct ttgtccggca
      481 gaatgagtgc tccgagtgtt ttctgatcct ggccgacgac gacacactga gcgacaaatg
      541 cactctgttt gattgaatct tcaatcggca gaggttctat gttttcacct cccagaagga
      601 caattgtatc ctttgcccgt cctgcaaatt tcagctgacc tcctgcagtc cagacgagaa
      661 tgtctcctga attcagccat ccgtcttcag tcagaacctg cttggtcttt tcaggttcct
      721 tgtaatattc cttcatgata tgaggccctc tgtgataggc aacacctttg acgttgggct
      781 gggtaactgt attcccctga ctgtctacaa gttttatctc aactccttca atcggagaac
      841 cgacagtgcc gacaactgtg ccgggcattt ttctgcgcac gctcacaccg actgtttctg
      901 tcattccgta tgtttcgatg ataggaactc ctatggagta gaaaagtctg tcgatgtatt
      961 ccggcagtgc tcctgcacca gagatggcaa acttgattct gtttccgagt gcacccttca
     1021 cttttttcaa tattgcctgt gcgagaatgt tcggaatgaa aagcagtatg catccgatca
     1081 atgctccgat tttttttgca cctgaaacaa taattgaatc cggttcaagt gaatatttca
     1141 gtccaagaag aatgtctttc agcgtgctga acttcagtgc tgtccagttg gcaaaattga
     1201 aaagggcttt tgcgaccgga gatgcctttg ctacgcttcc catcatctta ttgtaaaaac
     1261 tttcccagac tctgggcacg gacagcagaa atgtaggatt gactgcctga agatccttcg
     1321 caagtgaagg tacagaggtg aatgcaactg cacttactat acgtatacat gccatttcaa
     1381 tgagtctttc tgcaatatgc cagggaggaa gaaaccccat tgtcacacca aggggaggta
     1441 catccatccc tttcagcgag taatagactt cccaggtcag acttttctga gtatgcacga
     1501 ctcctttggg agcgcctgtt gtaccggatg tatagatgat tgttgcagta tcaggttcac
     1561 tgactgcttc ggcgagttta tgatattccc catctccgtt tttcagaagc gcttcccctt
     1621 tgttcatgag ctcggaaaga gtcattgcct gaaccgagga aggaattttc agagagctga
     1681 aatcttcgaa gaatacaatg tatttcagat tcttgaactg actgaggata gattcaattt
     1741 ttttatatac cttcactgtt tccaggaaaa taatcctgca gtcggcgtgc tggaaaatat
     1801 aggagagatc gccctgtgta gcgtctgttc ctcgcggaac atctactccg cctatggttg
     1861 tgattcccat acttacaggc atccatctgt gacctacgtc agcaatcaga ccgacgtttt
     1921 cccctttttt cattccgagg gaattcaatg ccagactgat ttttttaatc tcagtgaaca
     1981 tttcagagaa tgtttttgat ctgaaatcac ttgtgctgct ttctctgtac atctgggcgg
     2041 gagcattcgg atggctgtct gcagctgatt tgatcagctg atataaggta aatttcggcg
     2101 gcattgaatc cagttgaatc atctcggttc tctcgttaaa gtgatttggg aattttctgt
     2161 tcagaaaaat ctttctattg ataaggaatt attttattgg tttttgtaaa gaaaaaactg
     2221 aatctgcgtc tctttacagg cgatcggtgc cgaatatttc tgagactgca ggaagatcac
     2281 agattgcgaa tgggaaaagc tgtaatttct ttctggacag tcttcttcat ttatgcaatc
     2341 tgtatttctt gaacggatcg gaggcagata tgggagcatg gggtgtaggt tcagatgaaa
     2401 atgatgatac atttaatttt ttaggtgatt tctatcttcc gcaggcggac agaggttcaa
     2461 agaatccggc tgaaatctat gatgttcccg caatatacaa gtttcttaaa gaaacagaac
     2521 tgctttatga atacatatcc gatgtgggta tggtcgtgta ctttgcaaag aataaaaaag
     2581 catttccgaa tcttccgaag aaagctgtca agacaatcat agggtttata gatcaggaac
     2641 ttgcagagct gacatcagat ccattggtca caaccagaac ttgggatccg gcagattacc
     2701 gtgaaagaat ttccaagctg aaaaaagaga aaaaaattct ggaaaactat ctgaaaccaa
     2761 agacttcaaa aaagaaaaaa tgacctgcat aaagtatttt tttttacagc ggtagatatg
     2821 a
//
LOCUS       DAOQGJ010000303         2821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21804_length_2821_cov_2.523500, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000303 DAOQGJ010000000
VERSION     DAOQGJ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21804_length_2821_cov_2.523500"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            568..1671
                     /locus_tag="PK683_06045"
     CDS             568..1671
                     /locus_tag="PK683_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08044.1"
                     /translation="MTSKDLNSFIDYPYILYKEDPNWVPPLRIERREFFNPKKNPYFS
                     HSEVAYFLAYREGRIVGRVTAHEDKNWNSHYKSRQGFFGFYESEDDPETASALMQAAE
                     NWCKERGLKNIIGPMDFNSNHELGFQTYGFDSPPVFILKYTKEYYIKLFNDLNYHTVE
                     NLLSYDVPAQEPLPEKFAKMADRAKKLCGDFKIRNINMKKLRQELNPILEIYNQAWGE
                     NWGFIAMTPGEIDLLAEQFRLIADPEYIYILEKNGTIAGFLFALPDLNQALVSNRNGR
                     LFPMNFIKVYMNFRKINKGSIILLGVKKEFRNTGMELILMERLYSDGRRPPQKYKSIG
                     TGWILESNKPMNAIMNFLGGKIVKRYTVLSKDI"
     gene            1827..2087
                     /locus_tag="PK683_06050"
     CDS             1827..2087
                     /locus_tag="PK683_06050"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HNH08045.1"
                     /translation="MTAQCSTLISVPNWHKKNSPVLYSGTRCDVSLLIENDSGKSDPV
                     YAFDLPFSFVLDTVLLPVSIPLAYGFARVHKEIGFSEHCSEG"
     gene            complement(2268..2762)
                     /locus_tag="PK683_06055"
     CDS             complement(2268..2762)
                     /locus_tag="PK683_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH08046.1"
                     /translation="MKYAVSDGFPYYIELLGEADYLKLFSSLEDFELLETISEEKANY
                     RFAPDKWNIKQIVGHLTDHERIMIYRILRISRKDTAQLAGYDQELFTANSRFNEISYK
                     QILTDFKNVRASSISFIDTLSQEQLKFKGLVWKFEMSIEEFLKAVIGHTIHHMNIIKE
                     KYLK"
BASE COUNT          841 a          553 c          555 g          872 t
ORIGIN      
        1 acgactgcag gtttgataaa atagctccct aatgcaagcc cccccagtcc ctgcagtccg
       61 attaaaatta cacctagatg cttttttttc tctgatgaaa atttcctcaa ttgttccatt
      121 tgtctctcct ggtttgtgtc aaaatgaagt tagacagaca agtctttatt gcagggtagt
      181 aaggttctgc attagatgac tctttctgcg gctcggtcaa aaaagaatgt tcacggaatt
      241 ctgttcttca ggttcaattc agccccagat tcaaaatgct gatgactgaa tctccgcttc
      301 tctgcactct gtatcagaca gtcaattttt ttaaccgagg tttccatatt gtgcacaggc
      361 aaaaaatttt caaccaattt cctgaatact gcgtctcaaa atgaactgaa ttgatttggg
      421 aatggatagt atagaatttc aataatctgt cattcaggga aaaccctgaa tatataataa
      481 agtattgtag cagaaatcca tgcctgtaaa caggcctcta tgagcacaga aatgaattca
      541 ctaaataagt tattcataaa acaggaagtg acatcaaaag acctcaattc attcattgat
      601 tatccgtata ttctctacaa agaggatcca aactgggtgc ctccgctgcg cattgaaaga
      661 cgggaattct tcaaccccaa aaagaatcct tatttttctc attcagaagt ggcctatttt
      721 cttgcctatc gggaaggaag aattgtgggc agagtgactg cacatgaaga caagaactgg
      781 aattcccact acaaatccag gcagggattt ttcggttttt atgaatcaga agatgatcct
      841 gagacagcat ctgcactgat gcaggcggcc gaaaactggt gcaaagaaag aggactgaaa
      901 aacatcatcg gtccgatgga cttcaattca aaccatgaac tgggctttca gacatacggt
      961 tttgattctc cgccagtatt tattctgaaa tatacaaaag aatattatat aaaattattt
     1021 aatgatctga attatcacac tgtggaaaat ctgctcagtt acgatgtgcc tgctcaggaa
     1081 cctctgcctg aaaagtttgc aaagatggca gacagagcaa aaaaactttg tggagatttt
     1141 aagatcagga atatcaatat gaagaagctc cgtcaggaac tgaatccgat tctggagatt
     1201 tataatcagg cctggggaga aaactgggga ttcattgcaa tgacgcctgg agaaattgat
     1261 ctcctggctg aacagtttcg tctgattgca gatcctgagt atatctatat cctggaaaag
     1321 aacggaacga ttgccggatt tctattcgca cttcccgatc tgaatcaggc actggtttcc
     1381 aacagaaacg gcagactctt tccgatgaat tttataaaag tatatatgaa tttcagaaaa
     1441 ataaacaaag gatccattat tcttcttgga gtgaagaagg aattccggaa cacaggaatg
     1501 gaactgatac tgatggaaag actttattct gacggaagaa gaccgcctca gaaatacaag
     1561 tccatcggaa ccggctggat ccttgagagc aacaagccga tgaatgccat tatgaatttt
     1621 ctaggaggga aaatagtaaa aagatataca gttctctcaa aggatatctg aagaatttta
     1681 atcttgacag gactgatctg cagaataagt tttcccctat gattctgaga gtttctcgtc
     1741 aactaatttc ctttctgaag ctgctgtttc ccttaccgca cgctgatatg agacggagat
     1801 gtttgtgcaa gataatggtt ttgtatgtga ctgctcagtg ctccactctg atctcagttc
     1861 ctaactggca taaaaagaac agtccagtcc tgtattccgg tacaagatgt gatgtatctc
     1921 ttctgattga aaatgacagc gggaagtctg atcctgtata tgcatttgat cttccttttt
     1981 catttgttct ggatacagtg cttctgccgg tttccattcc gctggcctac ggatttgcaa
     2041 gggtgcacaa ggaaatcgga ttttctgaac actgttcaga agggtgagat tttgatcggt
     2101 ttgaatgtat tctgtttgta ttataattgc attatatctt attcagttca ttccggaaag
     2161 gactcagaaa ttatgaaatg aagcagttca gttattttca gttttagaga aaacatagcc
     2221 gccacagtat agcgttcaat gcataattct cagcctggaa tctgatttta cttcagatat
     2281 ttttctttaa ttatattcat atgatgaatc gtgtgaccaa ttactgcttt caggaattcc
     2341 tcaatactca tctcaaactt ccagaccagc cccttgaatt tcagctgctc ctgggataat
     2401 gtgtcgatga aacttatgct tgaagctctt acatttttaa aatcagtcag tatctgttta
     2461 taactgattt cattgaaacg gctgtttgca gtgaacagtt cctgatcgta acctgcgagc
     2521 tgtgctgtgt cttttctgct gattctcaaa attcgataga tcattattct ttcatggtca
     2581 gtaagatgtc cgacgatttg tttaatattc catttatcgg gcgcaaatcg gtaattggct
     2641 ttttcttcgc tgattgtttc cagcagttca aaatcctcca gtgaagaaaa taatttcaga
     2701 taatctgcct cccctaaaag ttcaatgtaa taaggaaaac catctgaaac tgcatatttc
     2761 attgcatatt ctcccttatt ctttttttaa tgggttccag tatggactcc agaccgttca
     2821 g
//
LOCUS       DAOQGJ010000304         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21815_length_2820_cov_3.840145, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000304 DAOQGJ010000000
VERSION     DAOQGJ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21815_length_2820_cov_3.840145"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /gene="leuC"
                     /locus_tag="PK683_06060"
     CDS             complement(<1..1248)
                     /gene="leuC"
                     /locus_tag="PK683_06060"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNH08047.1"
                     /translation="MSRTLYDRIFDSRILFEDGDETVLFIDRHFLHEVTSPQAFEGLR
                     LKNRKVKCPERCLATVDHNVSTQSKELDKVSDISRIQIQTLEKNCTDFNIPLIGFENS
                     ERGIVHVIGPELGLTLPGNTIVCGDSHTSTHGAFGALAFGIGTSEIEHVLCTQTLRTR
                     KMKNFKIRFHGFLNQGVSAKDLALHIIRRIGTSGGQGYVIEFQGEALSSFSMESRMTL
                     CNMSIEAGAKSAVIAPDRITFEYLYGRKYCPFKDFNDFESACRDLHSDPDAVFDQEIE
                     IYLDKIYPVVTWGINPAQSVQINESIPDPKTFADESEKKSAEYALAYMGLKPGMRVQD
                     ISIDKVFIGSCTNSRIEDLRKAASVMKGRKVHPEVKMLIVPGSRAVKKMAEQEGLDRI
                     FIESGAEWRDSGCSLCLGMNDDSL"
     gene            1596..2453
                     /locus_tag="PK683_06065"
     CDS             1596..2453
                     /locus_tag="PK683_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNH08048.1"
                     /translation="MEFRQIRYFMEIQNAGTFQKAAQNLGLTQPALSRQVFLLERELK
                     QSLFHRGPRQVKLTFYGEIFYEYSLKILELWNELDHKFSENAADLKGKYRISAGGTFS
                     ALILPDLLRRLHSDFPSLILSITEADGNEIESSLKQDRVEIGIVSEKIRDRDVISYEY
                     SEDTVVLAASKNYSIDADISSVNELKKHPFIYFQKGSAIRKLLDRTFSGKNKFHPNII
                     MEFRNIESVKNSIEKGLGIGFLSEMYMNSNLKKIRIPDLEIRRKFYICHKKDSEPGIR
                     LLVNKLLSY"
     gene            complement(2461..>2820)
                     /locus_tag="PK683_06070"
     CDS             complement(2461..>2820)
                     /locus_tag="PK683_06070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH08049.1"
                     /translation="RQADAGNLPFEENKFDILYNAYMLDLIPLKGFLPILTEFKRVLK
                     PGGKLVLLNMSKETEEETSFREKIYQFMPKMLSLYLMGGCRPVLMSDTVRKAGFRNIE
                     REFLKGNMPSEIVLAVK"
BASE COUNT          839 a          635 c          528 g          818 t
ORIGIN      
        1 cagagaatca tcattcatac ctagacagag agagcatcct gaatctctcc attctgcccc
       61 gctttcaatg aatattcggt ccagtccttc ctgctctgcc attttcttca ctgccctgga
      121 tccgggcaca atcagcattt tcacttcagg atgaaccttc cttcctttca ttacagacgc
      181 agcctttctt aaatcttcaa tcctggaatt tgtgcatgac ccgatgaata ccttgtcgat
      241 cgaaatgtcc tgcaccctca tacccggttt cagtcccata taagcaagcg catattctgc
      301 tgatttcttt tcactttcat cagcaaatgt cttcggatca ggaatggact cattgatctg
      361 aacggactga gccggattga ttccccaggt gacaacagga tatattttat ctaaatatat
      421 ctcaatttcc tgatcaaata ccgcatccgg atctgaatgc aggtccctgc aggcagactc
      481 aaaatcatta aagtctttga aaggacagta ttttcttccg tacagatact caaatgtaat
      541 acggtcagga gcaatgactg cagacttcgc acctgcttca atgctcatat tgcagagggt
      601 catcctcgac tccatactga atgatgaaag agcttctccc tgaaactcaa tgacataacc
      661 ctgtcctcct gaagtgccta ttctgcgaat tatatgcaga gcaagatcct ttgcagaaac
      721 tccctgattc aagaatccgt gaaacctaat tttgaaattc ttcattttcc ttgttctgag
      781 agtctgcgtg cagagcacat gttctatctc gcttgttcct atcccgaaag caagagcacc
      841 gaaagcaccg tgcgtagacg tatgggaatc accgcataca attgtatttc caggcagagt
      901 cagtcccagt tcaggtccaa tgacatgaac aatacccctc tcggaatttt cgaatccaat
      961 cagcggaata ttgaaatctg tgcagttttt ctccagggtc tgaatctgta tcctggaaat
     1021 atcggaaacc ttatccagtt ccttcgactg tgttgataca ttatgatcca cagttgcaag
     1081 acatctttca ggacatttga ctttgcggtt tttcagacgc agtccttcaa aagcctgagg
     1141 ggatgtcacc tcatgaagaa agtgacggtc gatgaataaa acggtctcat ctccgtcttc
     1201 aaatagaatc cttgaatcaa agattctgtc atacaaagtt ctgctcatat tttctccttt
     1261 gtcggaaagg cctgacagca tttccaaact tctgtctgaa tcattgcata tgttctgata
     1321 taatgcaaat aaatagcagt aatactgaat ataacagaaa gttatatcat atttcagttc
     1381 acttttttcg tatgtttatc atataaatat attaccaact cacctcacgc aggcggggcg
     1441 ttttggacag gaatgcctgc cggaggcttg aatccttccg gaaaccggaa aagtcagtaa
     1501 atttttatgc ttttacacag tatcgaaaac ggcattttag tcctgtcgcc tgagaatgca
     1561 ggttctcagc aaaaatatgc agccaaggaa gactgatgga attcagacag ataaggtact
     1621 ttatggaaat tcagaatgca ggaacatttc agaaagctgc acagaatctt ggactgactc
     1681 agcctgcact ctccagacag gtttttcttc tggaaagaga actgaagcag agcctgtttc
     1741 acagaggacc cagacaggtt aagcttactt tctacggtga gatattttat gaatactctc
     1801 taaaaattct ggaactgtgg aatgaactcg atcataaatt cagtgagaat gctgccgacc
     1861 tgaagggaaa atacagaatc tcagcaggcg gcacattttc tgcactgatc ctgcctgatc
     1921 tgctcagaag acttcattca gatttcccga gcctgattct ttccattacc gaagcagacg
     1981 gaaatgaaat tgaatcttca cttaagcagg acagagtgga aatagggatt gtttccgaaa
     2041 aaatccggga cagggatgtc atcagttatg agtattcaga ggatacagtt gttctggctg
     2101 cttccaaaaa ctattcaata gatgctgata tttcttctgt aaatgaactc aaaaaacatc
     2161 ctttcatcta ttttcaaaaa ggatctgcaa tccgcaaact tctggacaga acattttcag
     2221 gaaaaaacaa atttcatcca aatataatta tggagttcag aaatattgaa tctgtaaaaa
     2281 attcgattga aaaaggactc gggatcggat ttctttctga aatgtacatg aattcaaatc
     2341 tgaaaaaaat caggatacct gatctggaaa tcagaagaaa gttttatatc tgtcacaaga
     2401 aggattcaga accgggaatc aggcttcttg tgaacaaact tttaagttac tgatgtaaat
     2461 tcacttcacc gctagcacaa tttctgaagg catatttccc ttcagaaatt ctctctctat
     2521 gttcctgaaa ccggctttcc tcactgtgtc gctcatcagt acggggcggc accctcccat
     2581 cagataaaga gaaagcatct ttggcataaa ctgataaatc ttttccctga aagaagtctc
     2641 ttcttcagtc tccttgctca tattcagcag cacaagtttt cctcccggct tcagaactcg
     2701 cttgaactct gtcaggatag gaagaaaacc ttttaaagga atcaaatcga gcatatatgc
     2761 attataaagt atatcaaatt tattttcttc aaaaggaagg ttccctgcat ccgcctgcct
//
LOCUS       DAOQGJ010000305         2818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21855_length_2818_cov_2.581252, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000305 DAOQGJ010000000
VERSION     DAOQGJ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21855_length_2818_cov_2.581252"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PK683_06075"
     CDS             complement(<1..1151)
                     /locus_tag="PK683_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNH08050.1"
                     /translation="MKREYWKYAGVASVFLLLGTFLTPIIFHTISVQKEIHPLIAQGK
                     RDMSPGKSQAVHMEDAFQEVFDKVSPSVVSIATERTVKFKSPMGNDPFFDHFFGGNGG
                     GRPQEFKQKQTGLGSGIILNEEGYVLTNEHVVRDMEKLTVKLKNKKTYEAKLIGADKV
                     MDIALLKIKPGNDLVPVQIGDSSKVKVGHWAIAIGAPLGFEQSFTVGVVSAIARGVND
                     ASGVGYIQTDAAINQGNSGGPLLNIHGEVIGINRMIISQSGGFVGIGLAIPINDAKRI
                     AEELKTNGRIKRAWLGVSLDNVTDDDAKELKLPNTKGALVKQVVSGSPAESAGIELQD
                     VIVKIGEKDVEHPEDVVSYIMEAKIGKRVELKVQRKNSTLKMLVTPTERP"
     gene            complement(1295..1774)
                     /locus_tag="PK683_06080"
     CDS             complement(1295..1774)
                     /locus_tag="PK683_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08051.1"
                     /translation="MKVLFYGLIFLLGCTNYSVSRTVSVPPVLISVTPIASGHQILMR
                     AANPEIFFLGYKLYVGTSENDARNPASYTASGYNCDYRTVIPNLPVEYSAEVSSNSGG
                     LSSVAAGENANRVCKFAVSLNSAQYVSIRAMTFSFQGGRAGVQTISFSAPSNALVVP"
     gene            complement(1779..2336)
                     /locus_tag="PK683_06085"
     CDS             complement(1779..2336)
                     /locus_tag="PK683_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNH08052.1"
                     /translation="MDKLSEPVLILNSSYVPVSIRPVREAVCMMLSDKAEALKSSDED
                     FIRSEKLKIPVPRILLLSNYYSVPRKLLKLSRSAILERDGYTCVYCGKKPSPGKLTLD
                     HVIPKSRWMSIPQDKKPVEFNSWENLVTACRECNTRKGSKLLAELKWKLPENQKIKPK
                     QQYLPQISSNFAKKYGWSEYLGSET"
     gene            complement(2339..2803)
                     /locus_tag="PK683_06090"
     CDS             complement(2339..2803)
                     /locus_tag="PK683_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004496209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNH08053.1"
                     /translation="MHFMFGVGMAGVLVGDGVVFSAGYFFGPKILENKFVKKFVTPER
                     YNSVQNQFEKYGKWVVFMARFMPGLRTPIFLSAGISRRVTYARFLLTDGFAAIISVPI
                     WIYMGYFLAHNFNDLLAYMHRAQAVIFGLLGAAILFIVYKYLKKRRKRAEGA"
BASE COUNT          761 a          686 c          530 g          841 t
ORIGIN      
        1 ggacgttccg tcggagtcac aagcattttc agtgtagagt tttttctctg aaccttcagc
       61 tcgactctct tgcctatttt cgcttccatt atgtaggaaa caacatcctc aggatgttca
      121 acatcctttt caccgatctt cactattacg tcctgaagtt ctattcctgc agattcagca
      181 ggcgatccgg atacaacctg ttttacaagt gctccctttg tattcggcag cttgagctcc
      241 tttgcatcgt catctgtcac attgtcgagg gaaactccaa gccaggccct tttgattctt
      301 ccattcgtct taagttcctc agcaattctt ttcgcatcat taatcggtat tgccagtccg
      361 atccctacaa agccgccgct ctgagaaata atcatcctgt tgattccgat cacttccccg
      421 tgaatgttaa gaagaggtcc cccgctgttt ccctgattga ttgcagcatc tgtctgaatg
      481 taaccgactc cggaagcatc gtttacacct ctcgcaattg cactgacaac ccctactgta
      541 aatgactgtt caaacccaag cggagcaccg attgctatcg cccagtgacc tactttgacc
      601 ttcgaagaat ctccaatctg cactggaaca agatcatttc caggtttaat cttcaaaagc
      661 gcaatatcca ttaccttgtc agcaccgatc agttttgctt catacgtttt cttattcttc
      721 agtttgactg taagtttttc catatctctg actacatgtt catttgtaag tacatagcct
      781 tcttcattga gaatgatgcc ggaaccgaga ccagtctgct tctgcttaaa ctcctgaggt
      841 cttccgcccc cgtttcctcc gaaaaaatga tcgaagaaag gatcatttcc catcggactt
      901 ttgaatttca ccgttctttc agtggcaatg gaaaccacac tgggggacac tttatcaaag
      961 acttcctgaa aagcatcttc catatgaact gcctgagact tgccgggaga catatctctt
     1021 tttccttgag cgatcagagg atggatctct ttctgaacag aaattgtatg aaatataatc
     1081 ggtgtaagga aagtcccaag aaggagaaat acacttgcga ctcctgcata tttccaatac
     1141 tctcttttca taatgtttta ttacctcttt ttatttattc ttaaggccaa aagcccatat
     1201 taatctgcgt ccgaattccg attctgatgc agttcaccgg aagtccggtt acttattttg
     1261 actgtcattc ttaaaaacgg tttgcgtttt tttatcaggg aacgactagg gcattggaag
     1321 gtgcagaaaa gctgattgtc tgcacccctg ctctgcctcc ctgaaaactg aatgtcattg
     1381 cccggatgga tacgtactga gctgaattca gtgaaacggc aaacttgcat acacggtttg
     1441 cattttcccc ggccgcaaca ctggaaaggc cgcctgagtt agagctgacc tcggcagaat
     1501 attccactgg aagattcgga atcacagttc tgtaatcaca gttgtacccg ctggcagtgt
     1561 aactggcagg attcctagca tcattttccg atgtgcccac ataaagcttg taacccagaa
     1621 aaaaaatttc cgggttggct gctctcatca gaatctggtg accggaagca atcggcgtca
     1681 ccgaaatcag cactggcggc acagaaactg tcctggaaac agaatagttc gtacagccca
     1741 aaagaaaaat cagaccgtaa aacagtactt tcacaaaatc aagtttctga acctaagtat
     1801 tcactccaac catatttttt tgcaaaatta ctgctgatct gaggtaaata ctgctgttta
     1861 ggtttaatct tctggttttc aggaagtttc catttgagtt cggccagaag cttgcttcct
     1921 tttctcgtat tgcactccct gcaggctgtg accagatttt cccaggaatt gaattccaca
     1981 ggttttttat cctgaggaat ggacatccat ctgctttttg gaatgacatg gtccaatgtg
     2041 agctttcccg gagaaggttt ttttccgcag taaacgcatg tatacccgtc tctttccaat
     2101 atggcacttc tggaaagttt cagaagtttc cttggaactg aataataatt ggataaaaga
     2161 agtatcctag gaactggaat tttcaatttt tctgaacgta tgaaatcttc gtctgaggat
     2221 ttcagggcct cagccttgtc ggaaagcatc atacagacgg cctccctcac agggcgtatg
     2281 gaaacaggca cataagatga atttaaaatc aatactggtt cagaaagttt gtccattctt
     2341 aagctccttc agctcgtttt cttctttttt ttaaatactt atatactata aaaagtattg
     2401 cagcgcccaa gagtccgaaa atcactgcct gcgctctgtg catataagcc agcagatcat
     2461 tgaaattatg agcaagaaaa tatcccatat aaatccagat aggaacactg ataatcgcag
     2521 caaacccgtc tgtaagaagg aatcttgcat atgtcactct tctgctgatt cctgcactta
     2581 aaaatatcgg tgttctcagt ccaggcatga atctggccat aaagaccacc cattttccgt
     2641 atttttcaaa ctgattctga acagaattgt atctctccgg agttacaaat tttttcacaa
     2701 acttgttctc caggatttta ggtccgaaaa aataccctgc agaaaagaca actccgtcac
     2761 ctacaagtac ccctgccata cccaccccga acataaaatg cacattcgtg tccccaag
//
LOCUS       DAOQGJ010000306         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21894_length_2815_cov_3.736594, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000306 DAOQGJ010000000
VERSION     DAOQGJ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21894_length_2815_cov_3.736594"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2388
                     /locus_tag="PK683_06095"
     CDS             <1..2388
                     /locus_tag="PK683_06095"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNH08054.1"
                     /translation="KKTSIRLRTNSKRSERRENIEESLLQTNLGKDKELSFPVAKENI
                     AKSSEEVFKILAEKEEKVSVASNVVDDAQKQPVSVSVITRQQIKHSGARTLNELLTVY
                     VPGFFTVEDQDDTIAGFRGFAPDNNAKTLLLINGHNLNTEWFWGPPDSVINGMSLDYI
                     ERIEVIRGPGSVTLGQGALLGVINIITKKSDTAKGFSGSGMAGANGYQSGTLSGGFEG
                     KEIKDLKAFFQASTAKYNGQELRSEGWARSQPYAGAEGYYDWQRGFWDVDRTNMTASL
                     KKNVATSGSRLKRSNQDVVTSVIQYKGLEFTGFFTNQTRDMYNFYRDRNRLQNDVKHG
                     SVTYTYDWSEKLSAKFKTYYTQDDIFLRSNQGLTLGGTREYRYGGSMIFTLKDIIPNH
                     NIAVGTEFRKYDMGQKNSDGNNFILNYSQNTSDNAILLDSQGKGPNERNRYVYPGSIA
                     VKSIFMEDFYKLNSKWDIFGAFRYDRHPYWGSNLAPRIGTLYAYSNTLRFRLSYQEGF
                     RGVVGASYTGGFEGDGHLRIQNFPYVQPSAIPNSFDANGNPTGYYGDIAKTRPEKMRS
                     HEFAVNWDINSSWKMETIFFYNKAIQIIDVGVLYCDKPSTTMAGPNGCVMPRLGSDIP
                     GNWNGYWFYKNNPGQINQGGLETSITFKSKVFQSTASHSIVRLLSASSGQTDSIYLTS
                     DPTNRQFKGYPSNVFRWNSLYFASEKLTLGLTFLYYPSWYSPRNQRVDGNQLANFSIN
                     FRMLENLEMFAVIKNAFGNGNLYPMISNAGGKDISDGTPAVEKRTYWFGLNYTLF"
     gene            complement(2380..>2815)
                     /locus_tag="PK683_06100"
     CDS             complement(2380..>2815)
                     /locus_tag="PK683_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNH08055.1"
                     /translation="GGLVAVTAGCNNINPFSAILIGFIAGLLVVGGVILLDRLKIDDP
                     VGAVSVHGLCGLWGTVAVGFFANPAYGGNPEVKGLFFGGSFKLLGVQLLGAGLAFAVS
                     FGLGLLVFLALKYTIGLRVSKDEEIIGLDVLEHGNEAYPNSK"
BASE COUNT          861 a          590 c          650 g          714 t
ORIGIN      
        1 aaaaaaacca gcataagact tcgtacaaat tcaaagagat cggaaaggag agaaaatatt
       61 gaagagtcgc tccttcagac gaatttagga aaggataagg aactgagctt tcctgtggcc
      121 aaggaaaata tagcaaagtc ctctgaagag gttttcaaaa ttttggctga gaaggaagag
      181 aaagtttctg tggcttcaaa cgttgtagac gatgcacaga agcagcctgt atccgtttct
      241 gtaattacac gacagcagat caaacacagc ggcgcacgca ctttgaatga acttctgact
      301 gtttacgttc ccggcttttt tacggtggag gatcaggatg acactattgc aggtttcaga
      361 ggttttgctc ccgacaacaa tgccaagacc cttcttctga ttaacggaca taatttgaac
      421 actgaatggt tctgggggcc gcctgactca gtcatcaatg gtatgagtct tgattatata
      481 gaaagaatag aagtgatcag agggcctgga tcagtgactc tcgggcaggg ggctctttta
      541 ggggtaatca atattatcac taaaaagtcg gacacggcca aagggttcag cggttccggt
      601 atggcgggtg cgaacggcta tcagtctgga acgctttcag ggggattcga gggaaaggaa
      661 ataaaggacc tgaaagcttt tttccaggca tccactgcaa aatacaatgg gcaggaactt
      721 cgctctgagg gatgggcaag atctcagcct tatgcaggag ctgaaggcta ttatgactgg
      781 cagagaggct tttgggatgt ggacagaaca aatatgactg catctctcaa aaagaatgtg
      841 gcaaccagcg gaagcaggct caaacgttcc aatcaggatg ttgtaacttc agtgattcag
      901 tataagggac ttgagtttac aggttttttt acaaatcaga ccagggatat gtacaatttt
      961 tacagggaca ggaacagact tcagaatgat gtgaagcacg ggagtgttac gtacacttat
     1021 gactggtccg aaaaactttc tgcaaaattc aagacatatt acacacagga cgatattttt
     1081 cttcgttcaa atcagggact gactttggga ggaacaaggg aatacagata cggcggttcc
     1141 atgattttta cattaaaaga cattattccg aatcacaata ttgcggtggg gactgaattt
     1201 cgaaaatacg atatggggca gaaaaattct gacggaaaca atttcattct gaactattcc
     1261 cagaacacct ctgacaatgc aattcttctg gacagtcagg gaaagggacc aaatgaaaga
     1321 aacagatacg tgtatcccgg aagcattgca gtaaaaagta tttttatgga agatttttac
     1381 aagctgaaca gcaaatggga tatattcggt gcattccgtt atgacaggca tccttactgg
     1441 ggaagcaatc ttgctcccag gatcggtacg ctttatgcat attccaatac cctgcgtttt
     1501 cgtctctcct atcaggaagg attcagagga gtagtcggag catcgtatac aggaggtttt
     1561 gaaggagacg gtcatcttag aattcagaat ttcccgtatg tacagccctc tgccatcccg
     1621 aattcatttg atgcaaacgg gaatcctaca ggatattacg gagacatcgc caagacccgt
     1681 cctgagaaaa tgagaagtca tgaatttgca gtcaactggg atataaattc cagctggaaa
     1741 atggaaacta tattttttta taataaggcg attcagatta ttgacgtagg agttttgtac
     1801 tgtgataagc cttccacgac aatggcgggt cctaacggat gcgtgatgcc aagacttgga
     1861 agcgacattc ctggaaactg gaacggatac tggttttaca agaacaatcc aggacagatc
     1921 aaccagggag gtttggaaac aagcattaca tttaaatcca aagttttcca gtccacagcg
     1981 agtcattcca ttgtcagact tctttctgct tcatcaggac agacagacag tatttatctg
     2041 accagcgatc ctacaaacag acagtttaag ggatacccaa gcaatgtttt ccggtggaat
     2101 tctctttatt tcgcatctga aaaactcaca ctgggtctga cttttctcta ctacccttcc
     2161 tggtactctc cgcgaaatca gagagtggac ggaaatcagc tcgcaaattt cagcataaat
     2221 ttcagaatgc tggagaattt ggagatgttt gccgtcatta aaaatgcatt cgggaacggc
     2281 aatttatatc caatgatcag caatgccggc ggaaaggata tttccgacgg aacacctgcc
     2341 gttgagaaaa gaacctattg gttcggtctc aattatacac tattttgaat ttgggtatgc
     2401 ttcatttcca tgttccagca cgtccagacc gatgatctcc tcatccttgg aaacacgcag
     2461 tccaattgta tatttcagtg ctaggaagac aagcagtccg agtccaaatg aaactgcaaa
     2521 tgcaagccct gctcccagaa gctgaactcc aagcagtttg aacgatcctc cgaagaacag
     2581 tccttttact tcaggatttc cgccatatgc aggattcgca aagaacccga ctgccacagt
     2641 tccccataaa ccgcagagac catgcactga gactgccccg acaggatcgt caattttgag
     2701 tctgtccaga agaatcactc ctcctacgac aagcagaccg gcaatgaatc ctatcagaat
     2761 ggcagaaaaa ggatttatat tattgcaccc tgcagtcact gcaacaagac cgccg
//
LOCUS       DAOQGJ010000307         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21914_length_2814_cov_2.907213, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000307 DAOQGJ010000000
VERSION     DAOQGJ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21914_length_2814_cov_2.907213"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PK683_06105"
     CDS             complement(<1..877)
                     /locus_tag="PK683_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08056.1"
                     /translation="MKAKVILDISEELCDSDLPKEEIHKIADLIREHWEAYLHLDKKK
                     FSGKFSPSFRRMSQRARTIQDGTDAVLSGIQKEWELFERPNNIISDELTVRNLNIYAD
                     DKSSPSSAWLYYWIEIEGGSLWEYDDQGLVIQVLAKENGEWKLIHHTDGWSTDYDLDS
                     DECGSEPTFIFDYVYPVKDLGRAVKFYSAILGQPDWITETQAFFGLRDPGFILDSSGL
                     YGYSEIKKDLPNGYAVIYTEDLQKEIKKLKNHDVKFLENTDSEQKKKGNDLTALICDI
                     DGNVILISERNFSFSK"
     gene            complement(911..1480)
                     /locus_tag="PK683_06110"
     CDS             complement(911..1480)
                     /locus_tag="PK683_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08057.1"
                     /translation="MSQLGILKETVSVLKRENIPFMIVGSFVSSLYGEPRSTQDIDLV
                     IQLEKKHIPVLLSSFQPPKYYLDEELILMAMESKKMFNLLDTEEGDKIDFYIIKNEAY
                     EKEKFSRRINRMFLEEELPMLSAEDLIISKLIWCIQSGRSERQLRDAAGVYRVQKNAL
                     DLEYLERKIKELNLTDLYDEVKKGSVRDK"
     gene            complement(1459..1659)
                     /locus_tag="PK683_06115"
     CDS             complement(1459..1659)
                     /locus_tag="PK683_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08058.1"
                     /translation="MNSEEKYIETLRKMSFDEKAKKVFELTEMTRMLLKRGLEIQFPE
                     KTPEEIHQIYLRRLAECHNSEY"
     gene            complement(1664..2737)
                     /gene="lpxD"
                     /locus_tag="PK683_06120"
     CDS             complement(1664..2737)
                     /gene="lpxD"
                     /locus_tag="PK683_06120"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNH08059.1"
                     /translation="MNIHTEEILKKFQPQGLLESVQGKAEIQNIAPVETGHSGDLVFL
                     DKKEFLEFVIQNRPSAVVTSAEFADKVKDSADAVFISKNVGLAHAKIKTEYADRNWRD
                     IEEWGRIHPSAVIHPTVSVPESCVIGPKVVIGKNVKLGENCVFQANVVIERDAVIGND
                     VILCPNVFIGWGCQIGNRVLIHPGSVVGGEGFGFSTDSEKHYHRIPQTGIVVIEDDVR
                     IGTNCCFDRAAYSETRIKAGVKFDNLCHIAHNVIVGEDTVITAGGIVAGSTKVGKRVI
                     MSGQAGVLDHLTIADDVILLVRPGVMSDVKKPGVYAGTPLMPMSDFMKSQSVFRNLAE
                     LKMKVGRIEKQLNPKSDNEKKEI"
BASE COUNT          709 a          665 c          514 g          926 t
ORIGIN      
        1 cctttgaaaa ggaaaaattt ctttcggaaa tcaaaatcac atttccgtcg atatcgcata
       61 tgagggctgt aagatcattt ccctttttct tctgttctga atctgtattc tccagaaatt
      121 ttacatcatg attttttaat ttttttattt ccttctgcag atcttccgta taaatgactg
      181 catatccgtt cggaaggtct tttttgattt cggaatatcc ataaagaccg gagctgtcca
      241 gaatgaatcc cggatcccgg agtccgaaaa aggcctgtgt ctcagtgatc cagtccggct
      301 gacccagaat tgcagagtag aatttcactg cacggccgag atcctttacc ggatacacat
      361 aatcaaaaat gaaagtaggc tcacttccgc attcatcgga atccagatca tagtcagtcg
      421 accagccgtc tgtatgatga atcagtttcc attccccatt ctcttttgca agcacctgaa
      481 taaccagtcc ctgatcgtcg tactcccaga ggcttcctcc ttcaatttca atccagtagt
      541 acagccaggc agaagaaggt gaggatttat catctgcata gatattcaga tttctgactg
      601 tcagttcatc agatattata ttattcggtc tttcaaagag ctcccattct ttctgaattc
      661 cggaaagaac tgcatcagta ccatcctgaa ttgtccttgc tctctggctc atccgtctga
      721 aggaagggga gaacttcccg gaaaactttt tcttatccag atgcagatag gcttcccagt
      781 gttccctgat cagatctgca attttatgga tctcttcctt cggcagatca gagtcgcaga
      841 gttcttcact aatgtccagg attactttgg ctttcatatt cagctccttg atccgactta
      901 tactgggaaa ttacttatct ctcactgatc ctttcttcac ttcatcataa agatcagtaa
      961 gattcagttc ctttatcttt ctttctaaat attccagatc cagggcgttc ttctgtactc
     1021 tgtaaactcc tgcagcatcc cgaagctgtc tttcgcttct tcctgactga atgcaccaaa
     1081 tcagtttgga aataatcagg tcctccgcag aaagcatagg aagttcttct tcaagaaaca
     1141 ttctgtttat ccttctggaa aatttctctt tttcataagc ctcatttttg atgatataaa
     1201 aatctatttt atctccttct tcagtatcca gcagattgaa cattttcttc gattccattg
     1261 ccataaggat cagttcctca tccagataat acttgggcgg ctgaaaagaa gaaagcagta
     1321 caggaatatg ctttttttcc agttgaatta caagatcgat atcctgagta cttctgggtt
     1381 ctccgtagag acttgatacg aaggaaccga caatcataaa cggaatattc tctcttttta
     1441 agactgaaac tgtttctttc agtattccga gttgtgacat tcggcaagcc tccgcagata
     1501 aatttgatgg atttcttcag gcgtcttttc agggaactga atttcaagtc ctcttttcag
     1561 gagcatcctt gtcatttcgg tcagttcaaa aacttttttc gctttttcat cgaaactcat
     1621 ctttcggaga gtttcgatgt atttttcctc agaattcatg atattatatc tctttttttt
     1681 cgttgtcaga tttcggattg agctgctttt caatcctgcc cactttcatt ttgagttcag
     1741 ccagatttcg gaacacactc tgacttttca tgaagtcgct cataggcata agcggagttc
     1801 ccgcataaac acctggtttc ttgacatcgc tcattactcc agggcggacg agcaggatga
     1861 catcgtcagc gatagtgagg tgatcgagca cgcctgcctg tccgctcata atgacccttt
     1921 tccctacctt ggtggaacct gcaacgattc cccctgcggt tatgactgta tcctcgccta
     1981 caattacgtt atgtgcaata tgacagagat tgtcgaattt gactcctgcc ttgattctgg
     2041 tttcagaata agccgctctg tcaaaacagc agttggtgcc tatgcggaca tcatcttcaa
     2101 tgactacaat gcctgtctga ggaattctgt gatagtgctt ttctgaatct gtagaaaatc
     2161 caaaaccctc accgcctaca actgaacctg gatgaatcag aaccctgttt cctatctgac
     2221 agccccagcc gatgaataca tttggacaga ggattacatc atttccgatg actgcatctc
     2281 tttcaatgac tacgttcgcc tgaaatacac agttttctcc aagctttaca ttttttccaa
     2341 tcacaacctt gggtccgatg acgcaggatt ctggaacact cactgttgga tgaatgacag
     2401 ctgacggatg aattcttccc cattcttcaa tgtctctcca gtttctgtct gcatactctg
     2461 ttttgatttt ggcgtgagcg agacctacat ttttggaaat gaatactgca tctgctgaat
     2521 cctttacctt gtccgcaaat tcggccgatg tgaccactgc tgaaggtctg ttctgaatga
     2581 caaactccag gaattccttt ttgtccagaa agaccagatc tccgctgtgc cctgtttcga
     2641 ctggagcaat attctgtatc tctgctttcc cctgcactga ttcgagcaga ccctgcggct
     2701 gaaatttttt cagtatttct tctgtgtgaa tgttcatagt ccctttccaa cgtgatatta
     2761 ttagagttgt tgaaaaatta gtttcaacaa ctctattttt aatttttcta aaaa
//
LOCUS       DAOQGJ010000308         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_21962_length_2811_cov_3.383890, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000308 DAOQGJ010000000
VERSION     DAOQGJ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21962_length_2811_cov_3.383890"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..605
                     /locus_tag="PK683_06125"
     CDS             39..605
                     /locus_tag="PK683_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNH08060.1"
                     /translation="MFGTLPKIGRKAVNALGNTEGCIIVLDKSSSPKRVIKLIARGSM
                     SFFLTVKMFLAELFRKDNSISGEFESVRSNNELLVLLEKYRPSVVILFRAGLIINSKV
                     IDSGIPLLNIHCAKVPEYGGLGSISRALTDKAFDQCATMHRVTVKIDEGEVIDTLNYR
                     LDPSVSYSQNEEIAYNAGIELLKKRVKN"
     gene            1706..>2811
                     /locus_tag="PK683_06130"
     CDS             1706..>2811
                     /locus_tag="PK683_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08061.1"
                     /translation="MNVVLDYGVNWEHIFTAVTGVLDFETVLTLWKHTTEYHFYLSYA
                     IVAGSVLFLFSIRKTNATAFLILFLLAGINIYMYKQKKGDYQIVRLSDFISVLLPVFS
                     LYGYYLILKKIKYLPYILSGAFASLYLFLSVNDKKLFMRIAENDVVETRFSLKSSEDI
                     QAVSELSEIYESRKKAGKSPVFYYMGWGVVDSANNEIIYRKIPYFESIEYDYTPRGID
                     ILSKKYLEHALFVYPAGFLPDFLSVSSSYKAQTVSKIPGLKVFDATKGKGICLIGTGW
                     FPYHDNLLYMRDGLEKALVIWSDSDEAVLLNFSLLTAHMSFEGYISAVSDFEYEMPAG
                     YIDDHSVADKNEKHMKAKEFRIPLKRQSGELKVN"
BASE COUNT          829 a          457 c          588 g          937 t
ORIGIN      
        1 attacctgaa gttgcttagt atctgctcat ttacaacaat gttcgggaca ttgcctaaaa
       61 tcggaagaaa ggcagtcaat gcccttggaa atactgaggg ttgcataatt gttctggaca
      121 aatcgtccag tcccaagcgg gtaattaagc tgatcgccag aggatcaatg tcattttttc
      181 ttacagtgaa aatgttttta gctgaactct tccgcaaaga caacagtatc agcggagaat
      241 ttgaatctgt ccgatctaat aatgaacttc tggtactgct ggagaaatac agaccttccg
      301 tcgtaattct gtttcgggca ggactgatta tcaacagcaa ggtgatagat tcaggcattc
      361 cattgctgaa tattcactgt gcaaaagttc cagaatatgg cggacttggt tctatcagca
      421 gagctcttac ggacaaagca ttcgatcagt gtgcaacaat gcacagggta acagtaaaaa
      481 ttgatgaagg tgaagtaatt gatactctga actatagact ggatccttct gtatcctatt
      541 ctcagaatga ggaaatcgca tataacgcag gaatcgaact gctgaaaaaa agggtaaaaa
      601 actgatggca gaaccaagtg ttttaaatct ttatttgaag acagtgtttt ttcttctggt
      661 tttcggactg gctgggtggt ctgttttccc gcaaaagctg ttcaataggg acaattgtag
      721 taacttttta tattggatta tatcaggaat actgattttg tttctgtcag gttttataat
      781 gacaagactt tcagttagag gattatggat tcctgcagta gcattttttc tgatatcagg
      841 caggattgca ttcctcgtca tgaaaaaagg aaaaagagaa gatttttcat tcgttcagtc
      901 cagaatattt tggaaagagt tgtcaatatt tatagcagtt aatataataa tagcatccat
      961 ttttttcctt ccttttatga acagaaatac aagcggaata cttgcctatg gaggagggga
     1021 tcagtccagt tattttggtc tttcggaata tgcaaagtac catacttttg atgaagcttt
     1081 aagtgaagtg aaaggggatc cgcttcagat cctgcccggg caggcgaact ttgcagtaaa
     1141 atcgcttgca gcttatataa aaatcggtca gcagaatgtg tacgctcctc agactctcag
     1201 tcttccgttt cttgctatgg gatggggatc tgatgaggaa tcatatacag cagagacagc
     1261 agtatttata tgtttggcgg catggactgc cttgctttta gtgaacatca atctgaaaaa
     1321 aagaatacct gttttattat ttctgtttac tgttttaatt ggcggaataa gtaaaggaat
     1381 atacagtctg gccttaaaat ccgcacttcc tgctcttttt gtatggacaa tctgtctgtt
     1441 tgttctgtgt tgtctgcttt atataaaaga taggccgata gtcatcatac gatttccatc
     1501 attatatata ggcatttttt tagtatcagc tacagtgctt taccttccgt cattggttct
     1561 tttaatgcca gtgtatgctt ttttattttc agtcattcag aatcgggaat caatgatttg
     1621 gaaaaagatc agccttggtg ttcttttttg ttttatcatt gggaatttgg aattgctcag
     1681 accagtcaga ctgattcttt ccaatgtgaa tgttgtgctg gactatggag ttaattggga
     1741 acatattttc acagcagtaa ctggagtttt ggattttgag acagtcctta ctttatggaa
     1801 acatactacg gagtatcatt tttatctttc ttatgcaata gttgccggca gtgtcttatt
     1861 cctgttcagc ataaggaaga caaatgcaac agcttttctg attctgtttc tcttggctgg
     1921 aataaatatt tatatgtata agcagaagaa gggggactat caaatagtca gattgtctga
     1981 ttttatatct gtactccttc cagtcttttc tttgtatggc tattatttaa tattaaaaaa
     2041 gattaaatat ttaccttaca ttttatcagg agcatttgca agcttatact tgtttctgtc
     2101 agttaatgat aaaaaactct ttatgcgaat agcggaaaat gatgttgttg aaaccaggtt
     2161 ttcattgaaa agctccgaag atattcaggc agtatcagag ctgagtgaaa tctatgaatc
     2221 aagaaaaaaa gctggaaagt ctcctgtttt ttattatatg ggatgggggg tagtggacag
     2281 cgctaacaat gagataattt accgaaaaat cccttatttt gaatccattg aatatgatta
     2341 tactccgcga ggtatcgata ttctttccaa aaagtattta gagcatgcct tgtttgttta
     2401 tcctgcaggt tttcttcctg attttctaag tgtttcatct tcttacaaag cgcagacagt
     2461 atcaaaaata ccagggttaa aagtctttga cgcaactaaa ggaaagggga tatgcctgat
     2521 cgggacagga tggttcccat accatgataa tttattatat atgagagacg gtctggaaaa
     2581 agctttggtg atttggtcag attcagatga agctgttctt ttgaattttt ctctgcttac
     2641 agcccatatg tcttttgaag gatatatttc tgctgtgagt gattttgaat atgaaatgcc
     2701 agcaggatat attgatgatc atagtgttgc agataaaaat gaaaagcata tgaaagcaaa
     2761 agaattcaga attcccctga agagacagtc tggggaactg aaagtaaatt t
//
LOCUS       DAOQGJ010000309         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22115_length_2799_cov_2.603499, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000309 DAOQGJ010000000
VERSION     DAOQGJ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22115_length_2799_cov_2.603499"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..798)
                     /locus_tag="PK683_06135"
     CDS             complement(100..798)
                     /locus_tag="PK683_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08062.1"
                     /translation="MNWEETEISEDRTHHLLRGRPLYAARFVKVLEFHSNGTAAVTNN
                     TGSFHIDMYGKEKYKNKYDFTWGYYFGRAAVCDSSGAFHISETGESVYPERYEWCGNY
                     QECISSVRDFSRHYFHIDMDGRRIYKESYLYAGDFYEGIAAVRSADGLFRHIRKDGSE
                     LHTSYCLFLAQYHKGFALARDSSGWFSIDIYGKAVFEERFFYLEPFYNGSALAADNNG
                     NYCIVNTSGQKLLI"
     gene            complement(839..2521)
                     /locus_tag="PK683_06140"
     CDS             complement(839..2521)
                     /locus_tag="PK683_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNH08063.1"
                     /translation="MKPTFAIYGIKDRGDYEKTGYVHDHNLCLMQDGKVLKYLQLERF
                     TRRKYDNRLDLYLEDIINEHIPELRSEFDFISVNSFVGSSFISRNGRIRFEADRLNGL
                     STEPMKGRCWFERENWTGREIPAYLCSHETAHIFSCIPFYGLFNDNSLLVHFDGGASS
                     SNFSAFHWKNQKMNLLEHHWELSHLSKIFNDNSFTFRILGAKGGEHCSVPGKLMGYAG
                     MGKTDPLIEEWIIRNNYFRSFWNEPEIILQSINQEFGLSLDRFDERYDFFQNAAASFQ
                     SIFEKSILDKLNALQKKTGAEYLYYSGGSALNIRANSLILRSGIFKDIFIPPCCNDSG
                     LSLGAAAFLEYHKGSRIQHHSPFLNSIGCAFQSDVREESVNRSAKLLSEGKVLAVSLG
                     YGEVGPRALGSRSMLALADDRGLAEKINIRCKKREWYRPLAPVMLNSEAERILEEPVH
                     RLSEYMLLEYCVRKEYCKELNGVVHKNGTVRAQTVSSEKENPFLFRLLRLLNSKYGVK
                     ALLNTSFNCAGEPIVHNYDDAVKSAAKLGADALLTEKGLALSTEGFQNFIAE"
     gene            complement(2518..>2799)
                     /locus_tag="PK683_06145"
     CDS             complement(2518..>2799)
                     /locus_tag="PK683_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNH08064.1"
                     /translation="VVLENIVPLRQDGKYEVPYEYECPFLGRELWISAEGKISPCCAP
                     DEKRDTLGYFGNIKINSLENAVNSAEYQNLLNNYKSKHLCTICTMRKPV"
BASE COUNT          808 a          668 c          467 g          856 t
ORIGIN      
        1 taaatcccta tttagcttcg gatgaaagat aatttcttga ttttttcaac aagtcgattt
       61 tagagaaaga tgctcaaaaa gaatttttgt ccattccgtt cagattagga gcttctgccc
      121 tgacgtattc acaatgcaat aattcccatt attatctgct gccaatgcag acccgttgta
      181 aaagggttcc agataaaaga atctttcttc gaagacagct tttccataaa tatcaatact
      241 gaaccagccg ctgctgtctc ttgccaaagc aaagcccttg tgatactgag ccagaaaaag
      301 acagtatgaa gtatgcagct cgcttccatc cttccgtata tgacggaaaa gtccgtctgc
      361 agaccgcact gcggcaatcc cttcataaaa atctcctgca taaagatatg attccttgta
      421 tatccttctg ccgtccatat ctatatgaaa ataatgtctg ctgaaatctc tgacagaaga
      481 aatacattcc tgataatttc cgcaccactc gtatctttcc ggatacacac tttctcctgt
      541 ttccgaaatg tggaaggcac cagaactgtc acagacggca gccctgccaa agtaataacc
      601 ccaggtaaaa tcatatttat ttttatattt ctctttacca tacatatcaa tatgaaatga
      661 accagtatta ttcgttaccg cagcagttcc attggaatga aactcaagaa cttttacgaa
      721 tcttgccgcg tacagaggtc tgcctcttaa cagatgatgt gttctgtctt ccgaaatctc
      781 agtctcttcc cagttcaatc gttccgctcc acattatttt gaatattatt tacacttttc
      841 attctgcaat gaaattttga aatccttcag tactcagagc aagtcctttt tccgtcagaa
      901 gtgcatccgc accaagcttt gctgccgact tcactgcatc atcataatta tgcacaatcg
      961 gctcacctgc acagttgaaa gaagtattca gaagagcttt cactccgtat ttcgaattca
     1021 gaagccggag aagtctgaat aaaaaaggat tctctttttc agaagataca gtctgtgctc
     1081 ttactgtccc attcttatga acaacaccgt tcagttcttt acaatattct tttcttacac
     1141 agtattccaa aagcatatac tctgacagtc gatgaacagg ctcttccaat attctttcag
     1201 cctcggaatt aagcataacc ggtgcaagag gtctgtacca ttctcttttc ttgcatcgaa
     1261 tatttatctt ttcagcaaga cctctgtcat ctgccagtgc aagcatactc ctggatccaa
     1321 gagccctagg accgacttct ccgtatccca aggataccgc caaaactttc ccctctgaaa
     1381 gaagttttgc ggaccgattg accgactcct ccctgacatc cgactggaag gcacatccga
     1441 tgctgtttaa aaacggagaa tggtgctgta ttctgctgcc tttatggtat tccaaaaatg
     1501 ccgctgcccc cagactcagt ccgctgtcat tgcagcaggg cgggataaat atatccttga
     1561 aaattcccga tctgagaata agagaattgg ccctgatgtt cagcgcagaa cctcctgaat
     1621 aataaagata ttctgcgccc gtttttttct gaagagcatt cagcttatct aatatagatt
     1681 tttcaaatat gctttgaaat gacgcggctg cattctggaa aaaatcatat ctttcatcaa
     1741 acctgtcaag tgacaaaccg aattcctgat ttatcgactg aagtatgatt tcaggttcat
     1801 tccaaaatga tctgaaataa ttatttctaa taatccactc ttcaatgagc ggatcagtct
     1861 ttcccattcc tgcatacccc ataagttttc cgggaacact gcaatgttct ccccccttcg
     1921 ccccaagtat gcggaaagtg aacgaattgt cattgaatat cttagacaga tgcgaaagtt
     1981 cccagtgatg ctccagcaga ttcatcttct gatttttcca gtgaaacgca gaaaaattcg
     2041 aagaacttgc tcctccgtca aaatgcacaa gaagactatt atcattaaat aatccataaa
     2101 aagggataca agaaaaaata tgagctgttt catgactgca caaatacgct gggatttccc
     2161 gtccggtcca gttttccctc tcaaaccagc atcttccctt cattggttcc gtactgagac
     2221 cgttcagtct gtctgcctca aatcggattc tgccgttcct ggaaataaat gaacttccga
     2281 caaacgaatt cacactgatg aaatcaaact ctgatctcag ttcaggaata tgttcgttaa
     2341 taatatcttc taaatacaaa tcgagtcgat tgtcgtattt tctgcgggta aacctttcca
     2401 gctgcagata tttcagaacc tttccatcct gcatcagaca gagattatga tcatgaacat
     2461 aacccgtctt ttcatagtcc cctctgtcct taattccgta tattgcgaat gtcggtttca
     2521 tacaggtttt ctcattgtgc atattgtgca aagatgtttg ctcttataat tattcagcaa
     2581 attctggtat tctgcagaat tgacagcatt ttcaagtgag tttattttta tatttccaaa
     2641 atatcctaat gtatcccttt tctcatccgg agcacagcat ggcgaaatct tcccttccgc
     2701 agaaatccaa agttctcttc ccagaaacgg acattcatat tcataaggaa cttcatattt
     2761 tccatcctgt cttaaaggga caatgttttc aagaacaac
//
LOCUS       DAOQGJ010000310         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22235_length_2791_cov_2.669591, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000310 DAOQGJ010000000
VERSION     DAOQGJ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22235_length_2791_cov_2.669591"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..594)
                     /locus_tag="PK683_06150"
     CDS             complement(112..594)
                     /locus_tag="PK683_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNH08065.1"
                     /translation="MNRVTRVLGIDPGTHRTGFALLERSGRERPKVLEYGTWELKAKS
                     SHIESLLFLHEKIIQFTDKSSPDYAAVEEIFINRNLKTAAKVMQARGVILMSLAEKSV
                     NILEPTVTQIKKGITGKGNADKKDIRSAVKLFLGIDLEGHDDSWDAVACAFTGFSMIR
                     "
     gene            complement(591..2012)
                     /gene="lpdA"
                     /locus_tag="PK683_06155"
     CDS             complement(591..2012)
                     /gene="lpdA"
                     /locus_tag="PK683_06155"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNH08066.1"
                     /translation="MSAEFDVVVIGGGPGGYVAAIRSSQLGLKTAVVEMDRMGGICLN
                     WGCIPTKALLQSAHLLEEILHASEFGIKVEKASAEFSEIIKRSRSVADQMAKGVDFLM
                     KKNKVEVISGRAEFKDKNTLTVTSGTEKKEVSSKRFIVATGARSKPFNVLPFDQELVL
                     SSKEAMNQKGKPESIGIIGAGAIGVEFADFYASMGTKVHIIEFLDHLLPNEDEEVSVI
                     LERSFQKRGIQQYLGTGVYAAEKKKDSVVLKMKAKNDETKISEIEVSKVLVGIGLVPN
                     TENMNLESLGVVLNRGFIEVDERYRTHCDSVYAIGDCTGSPLLAHVASAEGIKASEAI
                     SIEFGNPHHLNYHVINYDYIPGCTYCHPEVASLGLTEAKAKAEGHDVKVGKFPFTASG
                     RARAAGDTTGMVKIVADAKHGEILGAHIIGANATEIISEIMLGASMELTVRNIMDTIH
                     AHPTISEGVMEAAAAVYGEAINI"
     gene            complement(2069..2350)
                     /locus_tag="PK683_06160"
     CDS             complement(2069..2350)
                     /locus_tag="PK683_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08067.1"
                     /translation="MNVFKSMILLFCVLFSFSCNTHEDNTKRNAVVSFLIACRGGSVS
                     ACYGMCNSDCGIGPTDSVTPTSLPCVSTCQSACQTGCSYGSLFITLTRK"
     gene            2485..>2791
                     /locus_tag="PK683_06165"
     CDS             2485..>2791
                     /locus_tag="PK683_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08068.1"
                     /translation="MFKYFILTSLSLFLLCTDLSAEVSAEIQTVNEAGLPEPDANIFV
                     EETKTAVRSDRNGRAVLRFPSLGFYSLRIVLSDRVISRSVHIRYDLQKIPLFTGIPSS
                     "
BASE COUNT          683 a          703 c          587 g          818 t
ORIGIN      
        1 caccgggaaa actgaccatc cctcagcttc tcctctccag aactccagtc aaaaaaggat
       61 tttccatatt cgagaaactt cctcctgaac ctaagaaaca gtgaaatcag ttcagcggat
      121 cattgagaaa ccggtaaatg cgcaggcaac tgcgtcccag gaatcatcgt ggccttcgag
      181 gtcgataccg aggaaaagtt ttactgcaga ccggatgtct ttcttgtctg catttccctt
      241 tcctgtaatc cctttcttga tctgagtgac tgtgggctcg agtatattca cgctcttttc
      301 tgccaaggac atcagtatga cgcctctggc ctgcatcacc tttgcagcag tcttcagatt
      361 gcggttgatg aatatctctt ccacagccgc ataatcaggg gatgatttat ctgtaaactg
      421 aatgattttt tcatgaagaa aaagcaggga ttcgatatga gaggattttg ctttcagttc
      481 ccaggttccg tattcaagaa ccttcggtct ttctcttccg cttctttcga gaagtgcaaa
      541 tcctgttctg tgagtgcccg gatcgattcc gagcactctt gtgactctgt tcaaatattg
      601 atcgcttcgc cgtagactgc agctgcagcc tccatgactc cttccgagat ggtagggtgg
      661 gcgtggattg tatccattat gtttctgaca gtcagttcca tagatgctcc gagcataatc
      721 tcggaaatga tctcagtcgc atttgcacct ataatatgcg caccgagtat ctctccgtgt
      781 tttgcatctg ccacaatttt caccattccg gttgtgtctc ctgcggccct tgcacgtccg
      841 cttgcggtga aagggaactt tccgactttc acatcatgac cttccgcttt tgccttggcc
      901 tcagtgagtc cgagagatgc cacctccgga tgacagtaag tgcaccctgg gatataatca
      961 tagttgatta catgataatt caggtgatga ggatttccga actcaatgga aattgcttcg
     1021 gatgccttga ttccttctgc agaagccaca tgagcgagaa ggggagatcc tgtgcagtca
     1081 ccgatggcat agacggaatc acagtgtgtc ctgtatcttt catccacttc gatgaagcct
     1141 ctgttcagca cgactcccag agattccaga ttcatatttt ccgtattcgg aaccagtccg
     1201 attcccacta gaaccttgct tacttcaatt tcagagattt tggtttcatc gttcttggcc
     1261 ttcattttca gaacgacaga atcctttttc ttttctgctg cataaactcc ggttcccaga
     1321 tactgctgta ttcctctttt ctgaaatgat ctttccagaa tgacactgac ttcttcatcc
     1381 tcgttcggaa gaagatgatc cagaaattca atgatatgaa ccttggtgcc catgcttgca
     1441 tagaagtcag caaattccac gccgatggca cccgcaccta tgatccctat gctttccggt
     1501 tttcccttct ggttcattgc ttctttgctg gaaagaacca gttcctgatc gaaaggaagc
     1561 acattgaacg gcttggaacg ggcaccggtt gcgacaatga aacgttttga ggagacttcc
     1621 tttttttctg tgcctgaagt tacagtgaga gtgttcttgt ccttgaattc agctcttcct
     1681 gagatgactt ccaccttgtt ttttttcatc aggaagtcca cgccctttgc catctgatcg
     1741 gcgacgcttc gggagcgttt aataatctct gaaaattcgg cagaagcctt ttccaccttg
     1801 attccaaact ccgatgcgtg taaaatttcc tccagaagat gagcactctg cagaagagct
     1861 tttgtgggaa tgcatcccca gttcaggcag atcccgccca tcctgtccat ttccactaca
     1921 gctgtcttca gtccaagctg ggacgatctg attgcagcca catatccccc cgggccgccg
     1981 ccgattacca ccacatcaaa ttctgcgctc attccttcct ccacaaacaa agtttttcaa
     2041 tcagattcag aaaggcttct tctgtcattc attttcttgt cagtgtgata aaaaggcttc
     2101 cgtaagaaca gcctgtctga caggcagact gacaggtgct gacgcaggga agagatgtcg
     2161 gagtgacgga atccgtcggt ccaatcccgc agtctgaatt gcacattcca taacaggcag
     2221 aaaccgagcc gcccctgcag gcaatcagga atgaaacaac agcatttctt tttgtattgt
     2281 cttcatgggt attgcaggaa aatgaaaaca gaacacagaa caggagaatc atacttttaa
     2341 aaacattcat agggaaattc ttttttacaa tcctttcagg tcaagcccga tctgctaaaa
     2401 tccaattgac agaatagagt ggaatttcgc agaatacctc aaatgatgaa atgccttgtc
     2461 cgtttcagct gaagatgatc tgatttgttc aaatatttta tccttacctc actgtccctt
     2521 ttccttctct gtacagatct gtctgccgaa gtcagtgcag agattcagac tgtaaacgag
     2581 gcaggtctgc ctgaacctga tgcaaatatt ttcgtggagg aaacaaaaac tgcagtgcgt
     2641 tcagacagga acggaagggc agttctccgt tttccttcac tgggctttta ttctctccga
     2701 atcgttcttt ccgacagagt gatttcgaga tccgttcata ttcggtacga tcttcagaaa
     2761 attccgctgt tcaccggaat accttcctct a
//
LOCUS       DAOQGJ010000311         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22253_length_2789_cov_3.263716, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000311 DAOQGJ010000000
VERSION     DAOQGJ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22253_length_2789_cov_3.263716"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK683_06170"
     CDS             complement(<1..277)
                     /locus_tag="PK683_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08069.1"
                     /translation="MPEKSLRELHKEAKAFLKGIAEDTKPSAEDWTLTACKQSISDDI
                     AVQLYWFSKTIAFFPADRHNSIESKQNKCAETFENEYKSQHLPIFNTL"
     gene            complement(279..644)
                     /locus_tag="PK683_06175"
     CDS             complement(279..644)
                     /locus_tag="PK683_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08070.1"
                     /translation="MTDPTGQYLQCRNGVACAPTSSLSIPTNQYIAVGSVQILNTTVN
                     EIWNGDLKGTISAVGGNFDYVAGAAPGTSYMPNGYLDADIAEVLFFNNALTQNDTDKI
                     FCYLRAKYNLSSVNKSCGI"
     gene            complement(742..1398)
                     /locus_tag="PK683_06180"
     CDS             complement(742..1398)
                     /locus_tag="PK683_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08071.1"
                     /translation="MNLSLFLISSVLILYCRPNFDRSLLEILFFRQQSQNSSIPNPAT
                     SPTVSSSKDITFYSIPSLGLNGIIAGTQISLTSDTLTAFTPLIAQFITTGKSVTVSGI
                     SQVSNVTANSYSSNLIYTVTAEDGSTKDFTVTLTAPRAYAGASLVIWLKADSLGLSDG
                     TAVSNWNDLSGYGNHYTQATAGLRPTYRVNQINGLPSVQFRFSLTQKYHCRRNKSVYK
                     "
     gene            complement(1587..1907)
                     /gene="trxA"
                     /locus_tag="PK683_06185"
     CDS             complement(1587..1907)
                     /gene="trxA"
                     /locus_tag="PK683_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNH08072.1"
                     /translation="MNNTEEKLPASFDELIRTHELPILVDFWAEWCGPCRMMTPVLSQ
                     LASEMKGKLTVIKINTDEKPALAAKYGISSIPTMILFKEGKEAGRTSGAMNLESLKKK
                     FALS"
     gene            complement(1977..>2789)
                     /locus_tag="PK683_06190"
     CDS             complement(1977..>2789)
                     /locus_tag="PK683_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNH08073.1"
                     /translation="ETSIMSDVESNLEILNGLNHLGMKIAVDDFGTGYSSLLQLLRLP
                     VSVIKIDREFVDGLDKRKDSRLITSAIIQMARALGKEIIAEGVENENQLLELKKNSCT
                     SIQGFYFHRPMDRNSAVQLLKDSIRILYNYDSLYRILYVSKAQNLSRIQLDEILNRSR
                     INNLMEGISGLLICNAGYFMQLLEGRKESVERLFQKISGDPRHINVEIVSEGETEQRM
                     FSRWSMGFWDMENENIKNLNRSPSQKKMTLWELANDPLMSLAFFEAISRTDL"
BASE COUNT          738 a          584 c          570 g          897 t
ORIGIN      
        1 gcagagtatt aaaaattgga agatgctggg atttatactc attttcaaaa gtctctgcgc
       61 atttattctg tttcgactca atagaattat gcctgtcagc tggaaaaaac gcaattgttt
      121 ttgaaaacca gtataactga accgcaatgt catctgatat actctgctta caggctgtca
      181 gagtccagtc ttcagcagat ggcttagtat cctcggctat tcctttcaaa aaagcttttg
      241 cctctttatg cagctctcta agactttttt ctggcatctc agattccgca gcttttattg
      301 acggaggata aattatattt cgccctgaga tagcagaaga tcttatcagt atcattttgc
      361 gtcagggcat tgttgaaaaa aaggacttct gcaatatcgg catccaaata tccgttcggc
      421 atataggaag ttccaggggc ggcccctgca acgtaatcaa aatttccgcc gactgctgaa
      481 attgttcctt tcaaatctcc gttccagatt tcatttacag tcgtattcaa aatctgtaca
      541 ctcccgacgg ctatatattg atttgtagga attgagagac tggatgtggg ggcgcaggcg
      601 accccgtttc tgcactgcag atactgtcct gtcggatcag tcaatgccat ttctctccca
      661 ttgcttccat ggatatttat aattgttgtt ccggcaggat ttgtagaaat cagtttgaaa
      721 acgataaaaa aagatccgct gttatttata tacagatttg ttcctcctgc agtgatattt
      781 ctgcgttaaa gaaaatctga attggacact gggcagtcca ttgatctgat ttactctgta
      841 tgtcggtcgc aagcccgccg ttgcctgtgt ataatgattt ccatacccgc tgagatcatt
      901 ccagttggaa acagcagtac cgtcgctcag accgagtgaa tctgccttca gccatattac
      961 cagagaagcc cctgcatagg ctcttggagc tgtcagtgtc acagtaaaat ccttagtgct
     1021 tccgtcttca gcagtaactg tgtatattaa gtttgagctg taagagtttg cggtcacatt
     1081 gctcacctga ctgattccgg aaacggtaac agattttccc gtcgttataa attgggctat
     1141 aagcggagtg aacgcagtga gggtgtccga agtcagactg atttgtgtgc cggcaatgat
     1201 tccattgagt ccaagagatg ggatggaata aaatgtaatg tctttgcttg aactgactgt
     1261 cggagaagtg gctgggtttg ggatggaaga attttgagac tgctgtctga agaataaaat
     1321 ttcgagaagg cttcggtcaa agttcggccg acagtataga attaaaactg aagaaatcag
     1381 aaataaactg agattcataa ctgaatgata aattgaatct tttattttgt aaagattttt
     1441 tataaatatt cagtatgact tcccagattc ctgatcttgc cagtatggaa gctgctactt
     1501 cgttcaatta gaactccagt tttctgttga ctgagccggt atcagaattc gtcagaactc
     1561 agtcaaataa accagctgaa tgatattcag gaaagagcaa actttttctt caggctttcc
     1621 aggttcattg cccccgatgt tctgcctgct tcctttcctt ccttgaagag aatcatcgta
     1681 ggaatggagc tgattccgta ctttgctgcc agtgccggtt tttcatccgt attgattttt
     1741 atgactgtga gttttccttt catttccgat gcaagctgac tcagaacagg agtcatcata
     1801 cggcaggggc cgcaccattc tgcccagaaa tccactaaaa ttggcagttc atgcgttctg
     1861 atcagttcgt caaatgaagc aggaagtttt tcttctgtat tgttcatttt attctccata
     1921 taccatggtg ggtatatgac cttctatacc atgccgggta tataagtcaa gaaaaatcag
     1981 agatctgttc tggaaattgc ctcaaaaaaa gcaagactca tcagcggatc atttgccagt
     2041 tcccaaagag tcattttttt ctgggaagga gatctgttca gatttttgat attttcgttt
     2101 tccatatccc agaatcccat actccacctg ctgaacattc tctgttctgt ttcgccttcg
     2161 gaaactattt ccacatttat atgtcttggg tctcctgata ttttttgaaa cagtctttct
     2221 actgattcct ttctgccttc cagaagctgc ataaaatagc ctgcattgca gatcaggagt
     2281 cctgaaattc cttccatcag attgttgatt ctggatctgt tgagaatttc atccagctga
     2341 attctggaaa gattctgtgc tttgctcaca tataatattc gatataatga atcataatta
     2401 tataatattc ttattgaatc tttgagaagc tgcactgcgg aattcctgtc catcggacgg
     2461 tgaaaataaa aaccctgaat gcttgtacag ctgttttttt tcagttccag gagctggttt
     2521 tcgttttcga ctccctcagc gatgatttcc tttcctaatg cccttgccat ctgaatgatt
     2581 gccgatgtaa tcagcctgct gtcctttctt ttatcgagac cgtctacaaa ttctctgtca
     2641 attttaatga ctgaaaccgg aagacgcaga agctggagaa gggatgagta cccggttccg
     2701 aaatcatcta cagcaatttt cattcccaga tgattcaaac cgttcagtat ttccaggttg
     2761 gattcaacat ctgacataat ggatgtttc
//
LOCUS       DAOQGJ010000312         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22255_length_2789_cov_3.220556, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000312 DAOQGJ010000000
VERSION     DAOQGJ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22255_length_2789_cov_3.220556"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PK683_06195"
     CDS             <1..1010
                     /locus_tag="PK683_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08074.1"
                     /translation="VMSIPLSSDPSDRLIWFREDIVKTVVWAGNKEEAYTVSNSRISP
                     RKSFERWIEAVKGKSEVWSKAYIRAVYKISGIIETIDKKIAEDSLQKTLLQIRKSEQE
                     LKQLNATKDKFFSIVSHNLRSPFSGLLGLSELLKASFSEAEPDMEEIKSYADLLYQSS
                     AQAFELLKNLFEWGKVQTGRMTIEKNQIDLKNILYELQYQLDGKLKEKDIHLRMEIGS
                     DLTLNTDENAIKSILNNLLLNSVKYSYPGKDIILRIQPMENCILIEIEDFGIGMNEGE
                     KQKLFKIDSKFRMPGTNGEDGTGLGLIITKEYLDRLNSKIEISTEKDYGTKVKLTFPR
                     E"
     gene            complement(1310..>2789)
                     /locus_tag="PK683_06200"
     CDS             complement(1310..>2789)
                     /locus_tag="PK683_06200"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNH08075.1"
                     /translation="SADGTDKNGTALTISGQSGSASLGVNPLTSTLQTALSSYAASDS
                     SLGLTAADIANISFLQTPTFIQPVPGISYTSPVSLNYPDTIYMFTQNQTNSAASPALS
                     GNLTGCTSSPSLPAGLSLNSLTCSITGSPTVGTALTAYTITGSNPAGSVTASVNIRTL
                     FGIPKFAYVPNYTSSNISVFTVDANTGSLTAGTTVNFGSNTRDVAVDPTGKYLYAAAF
                     TSNNIQLFTINSITGALTAYGSAVSAGTNPNSITVHPAGKFVYTANGGSNNISVFTVS
                     AADGTLTAGTPVSAGTNPRWIRIDPTGRMAYVVNSATNDINIYNINQTTGALTQYGGA
                     VSTGGDPRSIAVTPNGKFAYTADFNANHTNVFSVNLSTGALTAGTFVTPGTAPESVFV
                     HPNGLYLYTAHSGGGATGTISQYTINQTTGALTAGTVTGNGATIPGRVVIDPSGKFAY
                     STSFSTGNVTLYAVDQSTGGLTVGQAFTAGSGPNNIFVTGTN"
BASE COUNT          847 a          564 c          630 g          748 t
ORIGIN      
        1 gggtgatgtc cattcctctt tcctctgatc cgtcagacag acttatctgg ttcagggaag
       61 atattgtaaa aactgtcgtc tgggcaggaa ataaagaaga ggcatacaca gtaagtaaca
      121 gcagaatctc tccccgtaaa tctttcgaaa gatggatcga agcggtaaaa ggcaaatctg
      181 aagtatggtc gaaagcatat ataagagctg tatataaaat atccggaatt atagaaacta
      241 tagataaaaa aatagcagaa gacagtctgc agaagacact ccttcaaatt cggaaatcag
      301 aacaggaact caagcagctg aatgcaacaa aggataaatt tttctcaatt gtttcccata
      361 acctgagaag ccctttttcc ggtctgcttg gactgtctga acttctgaaa gcctcctttt
      421 ctgaagcaga accggatatg gaggaaataa aaagttatgc agatctgctt tatcagtctt
      481 cagcacaggc cttcgaactt ctgaagaatt tattcgaatg gggaaaagtt cagacaggaa
      541 gaatgacaat tgaaaaaaat cagattgatt tgaaaaacat actttatgaa ctccaatatc
      601 agctggatgg aaagctaaaa gaaaaagata tccatcttcg tatggaaatc gggagcgacc
      661 tgactctgaa tacagatgaa aatgcaataa agtcaattct gaacaacctg ctcctgaatt
      721 ctgtaaagta ttcctatcct ggaaaagata tcattctccg aattcagccg atggagaact
      781 gtattctgat agaaattgaa gacttcggaa ttggaatgaa tgaaggagaa aagcagaagc
      841 tttttaaaat tgattccaag ttccgtatgc ctggaaccaa tggagaggac ggtaccggtc
      901 ttggcctcat tatcactaaa gaatacctgg acagactgaa ttcaaaaatt gaaatcagca
      961 ctgagaaaga ttatggaaca aaggtaaaac tcacgtttcc aagagaataa ctccgatttt
     1021 aaattatatt ataattaaat tactaattca tcggttaaaa gtcaattctg gctggcactg
     1081 aagtgagcca aagaggagat taggcagttt ccgagtcgct gcataggcgt ctgcagaaac
     1141 ttctgtattc gcgaaatcgc tttggcctgt ccggcagacc gaagggcagg actccaaact
     1201 cgtatcggat tttcaagcga gcacagcaga gatttccaga cttgcgacgc ttcgccgtca
     1261 gaagtccggg gataatcagc ctttatgaaa tactttggga tataatgtat cagtttgtcc
     1321 ctgtaacaaa aatattgttg ggtccggatc ctgccgtaaa tgcctgtcca actgtcaatc
     1381 ctccggttga ctgatccact gcatacagag ttacgtttcc tgtcgaaaag cttgtgctgt
     1441 aggcaaattt gccggaagga tcaataacta ctcttcctgg aatcgtagca ccgtttcctg
     1501 tcacagttcc tgcagtcaga gcacctgtag tctgattgat agtatactgc gatattgtcc
     1561 ctgtagctcc cccgcctgaa tgtgcggtgt aaaggtaaag tccgtttgga tgaacaaaaa
     1621 ctgattccgg tgctgttcca ggcgtgacaa aggttcccgc tgtcagcgct ccagtactga
     1681 gatttacgga gaatacattt gtatgatttg cattaaaatc agctgtatag gcaaattttc
     1741 cgttcggagt gactgcaatg cttctaggat ctccccctgt agaaactgct cctccgtact
     1801 gggtcagtgc tccagttgtc tgattgatat tataaatgtt tatatcatta gtagctgaat
     1861 tgacaacata ggccatcctg ccagtcggat ctattctgat ccatcttgga tttgttcctg
     1921 cgctcaccgg agttccagcg gtaagagttc catctgctgc acttactgta aaaacagata
     1981 tattattgct ccctccattt gctgtatata caaattttcc agcaggatga actgtaatgg
     2041 aatttggatt tgttcccgca gacacggctg aaccgtaagc agtcagtgct cctgtaatac
     2101 tattgattgt aaaaagctgg atattattgc ttgtaaaagc agccgcatac agatattttc
     2161 ccgtcggatc aactgcgaca tcccttgtat tcgacccaaa atttactgtg gtgcctgcag
     2221 tcagagaacc ggtattcgca tctacagtaa aaacagaaat attgctggaa gtataattcg
     2281 gaacgtatgc aaatttagga attccaaaga gagtgcggat atttaccgat gcagtcacgc
     2341 tccctgcagg gtttgatcct gtaattgtat atgcagtgag agcagttccg accgttggag
     2401 atccagtgat gctgcaggta aggctgttca ggctgagacc tgcgggcaga gaaggactcg
     2461 aagtgcagcc agtaagatta ccggacagag ccggggacgc agctgaattc gtctgattct
     2521 gagtaaacat atatatagta tcaggataat ttaatgacac tggagaagta taactgatgc
     2581 cgggaacggg ctgaatgaat gtaggtgtct gcaaaaagga tatatttgca atatcagctg
     2641 cagtcagtcc cagactggaa tctgatgccg cataactgga aagtgctgtc tgaagagtgg
     2701 aagtcagcgg attgactccc agagaagctg aacctgactg tccggatata gtcaaagcag
     2761 ttccattctt atcggttcca tcagcagat
//
LOCUS       DAOQGJ010000313         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22281_length_2787_cov_4.217789, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000313 DAOQGJ010000000
VERSION     DAOQGJ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22281_length_2787_cov_4.217789"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK683_06205"
     CDS             <1..214
                     /locus_tag="PK683_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08076.1"
                     /translation="YINENAEKTKLYAMRSKLKEENLPGTKVYMHRFILNVKDKSVAV
                     KHRNGNTLDNRRDNLYSTKRKLAKKK"
     gene            211..708
                     /locus_tag="PK683_06210"
     CDS             211..708
                     /locus_tag="PK683_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNH08077.1"
                     /translation="MKKLFTHLFYPGFMVKKYFTKEVLDSIELVIKDSETKHSGEICF
                     AVEGSFSADKIFQHFDSRKRALEVFSNLRIWDTEGNSGVLIYVLLAERKIEIIADRGI
                     SSQVNQKVWDEIAESMSSYYSKGLYEKGSVNGIMQVTELLSQKFPNSSPSTKKNEISD
                     KPVLL"
     gene            complement(709..2709)
                     /locus_tag="PK683_06215"
     CDS             complement(709..2709)
                     /locus_tag="PK683_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNH08078.1"
                     /translation="MPKIHKLLIANRGEIALRIIRTCNRLNIKTVAVYSEADKDYPFV
                     KAAGESYLLGTAEASKSYLNAEKLIEICRTSGADAVHPGYGFLSENEGFAKALQTAGI
                     IFIGPSSDAIRMMGDKTESRKKMKETGVPVIPGYDGSNQDEKFLQEEAEKIGYPVMIK
                     ASAGGGGKGMRRVDRKEDFLESLYAAKREAKNFFSNDSVFLEKYVLSPRHIEVQIFGD
                     SHGNYAALHDRDCSVQRRHQKVIEEAPAPQLPENIRKEIRDASILAASSIRYENAGTV
                     EFVYSDGKFYFLEMNTRLQVEHPVTEMITGLDLVELQIRTAEGEKLPFTAETVPPVKG
                     HAVELRLYAEEHGLPRSGKIHTFGTTEGLTEGQRIDSGVESGSYVSVHYDSMIAKLIS
                     FGKNREEAIQNALSLTEKNIVFGIPVNHSFLNAVLKHKKFREGGVNTGFLEEYSEDLR
                     KTEETNESELAECAFLAHTVSSTVFEGVKGNIAGRKFLWEEKKTENPVKVLLNRELIL
                     ETKTYLRNGEEFVAEAVSDDEIRITHQSFQKTFSIRALTVSQKNILHWNGRNVFYERY
                     SAHIYLHHLGKNWNFHLKIQGNSGSKNSGFKSPMPGKIISVRVRPGDNVVEGDILMIV
                     EAMKMENQIRAYSDLSIEEVYFKEGDLVTGDDQLLKAIELDS"
BASE COUNT          693 a          633 c          567 g          894 t
ORIGIN      
        1 gtatattaat gaaaatgcag aaaagactaa actttatgcg atgcgttcca agctgaaaga
       61 ggaaaatctt cctggcacga aggtgtatat gcacaggttt attctgaatg taaaagacaa
      121 atctgtcgct gtcaagcaca gaaacgggaa caccttggat aacagaagag acaacttgta
      181 ttctactaaa cgcaagctgg ccaagaaaaa atgaaaaagc tttttactca tcttttctat
      241 ccaggattca tggtaaaaaa atattttact aaggaagttc tggattcaat tgagcttgtc
      301 attaaggact cagagacaaa acactcgggc gagatctgtt ttgcggtaga aggatctttt
      361 tctgcggata agatctttca gcactttgat tccaggaaaa gggctttgga ggttttttcc
      421 aatcttcgta tctgggatac tgagggaaac tcaggagttc tgatttatgt tcttttggca
      481 gaaagaaaaa ttgaaatcat tgcggacaga ggcatttctt ctcaggtaaa tcagaaagtc
      541 tgggatgaga ttgcggagtc gatgagttcc tactacagca agggtcttta cgaaaaaggt
      601 tcggtgaacg ggattatgca ggtcacggaa cttctcagtc agaaatttcc taattccagt
      661 ccttcaacca agaaaaatga aatttccgac aagccggtgc ttctgtaatc aggagtccag
      721 ctctattgct ttcagaagct gatcgtctcc ggtaacaaga tcaccttcct tgaagtagac
      781 ctcttcaata ctcagatcag agtatgctct gatctggttt tccatcttca ttgcttccac
      841 gatcatcaga atatcccctt ccacaacatt gtctccaggc cttactctga cagagatgat
      901 tttgccgggc attgggcttt tgaatccgct gtttttggat ccgctgttcc cctgaatttt
      961 cagatggaag ttccagtttt tccccaaatg gtgcagatag atatgagctg aatatctttc
     1021 ataaaagaca ttccttccgt tccaatgcag aatatttttc tgtgaaactg taagtgctct
     1081 tattgaaaat gtcttttgaa acgactgatg ggtgattctg atctcgtcat cggaaacggc
     1141 ttctgcgacg aattcctcac cgtttctcaa atacgttttt gtttcaagaa tcagttctct
     1201 gttcagaagg acttttaccg ggttttcggt ctttttctct tcccagagaa atttccttcc
     1261 tgctatgttt cctttaacgc cttcaaagac tgttgaactt actgtatgag caagaaatgc
     1321 acattctgca agttccgact cattcgtttc ctcagtcttc ctgagatcct ccgagtattc
     1381 ttcaagaaat cctgtattga ctccgccctc tctgaatttt ttatgcttca gaactgcgtt
     1441 caggaaagag tgatttaccg gtattccgaa aacgatattt ttttctgtta aggaaagtgc
     1501 attttgtatt gcttcctcgc ggttttttcc gaaggaaatc agttttgcaa tcatggaatc
     1561 atagtgaaca gaaacatagg atccagattc cactcctgaa tcaattctct gtccctcagt
     1621 cagtccttcc gttgtgccga aagtatgtat ctttccgctt ctcggcagac cgtgctcctc
     1681 tgcgtaaagc ctgagttcca ctgcgtgacc ttttaccgga ggaacagtct ctgcggtaaa
     1741 ggggagtttt tctccctctg cagttctgat ctgaagttcc acaagatcca gacctgtgat
     1801 catttcagtc accggatgtt ccacctgcag tcttgtgttc atttccagga agtaaaattt
     1861 tccgtcagag tatacaaatt ccacagtccc tgcattttca tagcgaatgg aggaggccgc
     1921 gaggatggat gcatctctga tttcttttct gatattttca gggagctggg gagcaggagc
     1981 ttcttctatg accttctggt gtcttctctg aactgaacag tctctgtcgt gaagagctgc
     2041 atagttcccg tgagaatccc cgaagatctg cacttctatg tgtcttgggg atagaacata
     2101 tttttccagg aatacagaat cattagagaa gaagtttttt gcctctctct tcgctgcata
     2161 cagagattca agaaagtctt cctttctgtc cactctgcgc attccctttc cgcctccgcc
     2221 cgcactggct ttgatcatta cagggtatcc gattttctct gcctcttcct gcagaaattt
     2281 ttcatcctga ttgcttccgt catagcctgg aataacagga actcctgtct ctttcatttt
     2341 ctttcgggat tctgtcttgt ctcccatcat tcggattgca tcagaactcg ggccgatgaa
     2401 tatgattcct gcagtctgca gtgcttttgc gaatccctca ttttctgata aaaatccgta
     2461 accgggatga actgcatccg ccccggaggt tctgcagatt tcaatcagtt tctccgcatt
     2521 cagataggac ttggacgctt ctgcagtacc gagcaggtaa gactctcctg cggccttcac
     2581 aaaaggataa tccttgtctg cttccgaata gactgcgact gtctttatgt tcagtctatt
     2641 gcatgtacgg atgattctaa gtgcaatttc acctctgttc gcgatcagca gtttatgaat
     2701 tttgggcatt tctttcttct acttctttat ccagaatttt cttttcttca accattctgt
     2761 catgcacgtg atccctgaat tcttccc
//
LOCUS       DAOQGJ010000314         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22363_length_2781_cov_3.820249, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000314 DAOQGJ010000000
VERSION     DAOQGJ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22363_length_2781_cov_3.820249"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK683_06220"
     CDS             complement(<1..505)
                     /locus_tag="PK683_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNH08079.1"
                     /translation="MGKNNVRTILLVAFAALVVLILSYNSAKYGSTVDEISYSDFLNM
                     VEPSEDKKPIGKILISDGKNKKQLLIDREIVEGWYIPENSEDKKPHQFKTLIAPVHQD
                     LVTKLRKSNLAFQAKNPDDSKFLNGLISTLPWILVAVVFWFFMMRQIQSTGSKAFTFG
                     KSRARLNV"
     gene            792..1817
                     /locus_tag="PK683_06225"
     CDS             792..1817
                     /locus_tag="PK683_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08080.1"
                     /translation="MTQFLLLLTKTGSLAGTAPSSSKAITSYSIPSLGITGTISGTSV
                     YLLADQLTSMTPLTASFTSTGKKVTVGNTEQTSGVTSNSYSSSLIYTVTAEDGSSQNY
                     TVTLTAPKIYGGGSLRIWIRSTSLGLSDGAQIASWTDESGYGNHFTQGTPANRPIFKA
                     GQINGLPAARFTQSAGSNMSISAPVSMYPDAQNGSFLIVLSSAGYTVEQVILNLSGAN
                     GRQFSLSPTPSSAFLQGRNGVGYNYSSSFLIPLNTFLSIGSVQSGAASVNEYWNGDLK
                     GSNAPSCCSNYVTGGSVSITNGALNGDIAELMYFATPLSSDEMKRLFCYIRGKYGFSA
                     SDTGCGL"
     gene            complement(1822..>2781)
                     /locus_tag="PK683_06230"
     CDS             complement(1822..>2781)
                     /locus_tag="PK683_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNH08081.1"
                     /translation="RYSQNLQYRRNSDGNPYSRYETDTMYNRYISEISGSNVNVDRII
                     FRVIRLIGGTSFSRNIVRTYDGKPVKTDELYFPDSGSDLKEIYIKSRLYANTMNGRTK
                     LTEDFEKGRITGFHGGYDNTVRIGISLDRRDFSVDPTKGYFLELAHDRSLKIIGSDFE
                     YNKTFAQGRFYARLFPSALERTVLALRAGIVSTKGSAPFFSYPNMNVTESYMIGLGGG
                     STLRGYQLSRFVGPVMGIANLEIRSRFASWSHSDHYFTFHFIPFYDIGRVWDRFSKVS
                     STGYAASYGIGLQISYNLSSVFRLDLAKSREDSTVNLQMMHPF"
BASE COUNT          757 a          651 c          594 g          779 t
ORIGIN      
        1 ctacattcag tctcgctctg cttttcccga aagtaaatgc cttgcttcca gtggactgaa
       61 tctgtctcat cataaaaaac cagaatacga ctgcaacgag aatccaggga agagttgaaa
      121 taagaccgtt cagaaacttg ctgtcatcag gatttttcgc ctgaaatgcc agatttgact
      181 ttctcagttt agtgaccaga tcctgatgca caggtgcaat cagtgtcttg aactgatgag
      241 gctttttgtc ctctgaattt tcagggatgt accagccttc tacaatttct ctgtcaatca
      301 ggagctgttt cttgtttttc ccgtcggaaa tcagaatctt tccaatcggc tttttgtcct
      361 ctgagggctc caccatattc agaaagtctg aataagagat ctcgtccaca gtggaaccgt
      421 attttgctga attatagctt aaaatcaaga ctaccagtgc ggcgaatgct accagaagaa
      481 ttgtccggac attgttttta cccattatct actccgtctg ggataagttt gagaaaattg
      541 cttatactgg caagagtttt actaaaaaaa ctatgataat ttacccttca gttgtcgata
      601 gttagggcgg tctctttcaa tgtttgaaga gaaagaaact gatcagcgga attcagcttt
      661 tccaatgaca gtatactttt acgatatccc aaatgaaaaa aatcatcatc tgccttatcc
      721 tgtattcctt tccgggctgt cttaagacga aacggacggc acttgacgtc aggagttccg
      781 ccggatctgc cttgacccag ttcttacttc ttctgaccaa aacaggatct ctggcaggga
      841 cagctccgtc ttcatccaag gcgatcacat cctattccat tccgtcactg ggaatcaccg
      901 ggacaatatc ggggacctcc gtttaccttc tggcagatca gctgacttca atgactcctc
      961 tcaccgcaag ctttacatcc acaggtaaaa aagtgacagt aggaaatact gagcagacga
     1021 gcggagttac ctcaaattcc tactcctcca gtctgattta tacagtgact gctgaagacg
     1081 ggtcttctca aaattataca gtgactttga ctgcaccgaa aatttacggg ggaggatctc
     1141 tgcgcatctg gatcagatcc acttcacttg gactcagtga cggggcacag attgcctcct
     1201 ggactgacga aagcggatat ggaaatcact ttacacaggg aactcctgcg aaccggccga
     1261 tcttcaaagc gggccagata aacggtctgc ccgcagcaag atttactcag agtgccggat
     1321 ccaatatgtc catttcggct cctgtatcaa tgtatcccga cgctcagaat ggatccttcc
     1381 tcatcgtctt aagttcagca ggatacactg tagagcaggt cattctgaat ctgagcggtg
     1441 caaacggaag acagttctct ctttctccga ccccttcttc agcatttctt cagggaagga
     1501 atggtgtagg atacaattac agttcgagtt ttctcattcc tttaaacaca ttcctgagca
     1561 tcgggagcgt tcagagcggt gctgcaagtg tcaatgaata ctggaacgga gatctgaaag
     1621 gaagcaatgc tccctcctgc tgctcgaact atgtgacagg cggaagtgtt tcgattacga
     1681 acggtgcgct gaacggagat attgctgagc tgatgtattt tgcgacgcct ctttcttcag
     1741 acgaaatgaa aagacttttc tgctatatca gaggcaagta tggattttca gcatctgata
     1801 cagggtgcgg actgtaggtc tttaaaaggg gtgcatcatc tgcagattga ctgttgaatc
     1861 ctctctggac tttgcgagat ccagtctgaa tactgaactg agattgtacg aaatctgcag
     1921 accgattccg taacttgcag cataaccggt cgaactgact ttggaaaatc tgtcccagac
     1981 acgccctatg tcgtaaaacg ggataaaatg aaacgtaaaa taatgatcgg aatgactcca
     2041 ggatgcaaac ctgctccgaa tctccagatt ggcaattccc atcacaggac cgacaaaacg
     2101 ggaaagctga tatcccctga gcgtggaacc tcctcccagt ccgatcatat agctttcagt
     2161 cacattcata ttcggatagg agaaaaaagg agcactcccc ttggtggaaa caattcccgc
     2221 acggagagcc agaactgtcc tttccaatgc ggaaggaaaa agtctggcat aaaatcttcc
     2281 ctgtgcaaat gtcttattgt attcaaaatc agaaccgatg attttcagcg atctgtcatg
     2341 tgccagttcc agaaaatatc cctttgtcgg atccacggaa aaatccctcc tgtcaagcga
     2401 gatgcctatc cgcactgtat tgtcataacc gccatgaaag cctgtaattc ttcctttttc
     2461 aaaatcctct gtcagttttg tccgcccatt catagtattt gcatataatc tagattttat
     2521 ataaatttcc ttcaaatctg atccagaatc aggaaagtac agttcatctg tcttgacagg
     2581 ctttccgtca tatgtgcgga caatgtttct ggaaaaggag gtccctccga tcagtctgat
     2641 tacacggaaa atgattctgt ctacattgac atttgaaccg gaaatttcgg aaatatagcg
     2701 gttgtacatt gtatctgttt cataccggct gtatggattt ccgtctgaat ttcttctgta
     2761 ctgaaggttc tgcgagtaac g
//
LOCUS       DAOQGJ010000315         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22366_length_2781_cov_3.378210, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000315 DAOQGJ010000000
VERSION     DAOQGJ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22366_length_2781_cov_3.378210"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            657..2558
                     /locus_tag="PK683_06235"
     CDS             657..2558
                     /locus_tag="PK683_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH08082.1"
                     /translation="MKNFRFSFGFKIGMALSSLSMLISAAGIAFLYNYTYRALIEQVA
                     KKTVEIGQTSAYLFQEEEREDIKFLRSISEARMQSLTEFDLAIEPGETKPSIPDDIAD
                     EIMKGDEFQKIVQLLRSIKAASMDQVQPLGFLQQITPEKPAKFRYVYMLAEIPQVPSR
                     KVLKFIADADYEAIDENGDGKISEEEKAVPIGTLYSVNSREMINAFDGNSYYSREIYT
                     DKWGKFIGSMVPIKDSDGKVLAILCMEYEVSEVLKQTKQILYISSGIIFASFLLSLLF
                     SSIVAYWIAKPINALKDAAEKVSNQNYNVSITTDRQDEIGVLSRTFGSMVREIQEFTR
                     KLEESNMHLQRLNQSYKRFVPQEFLGFLKKKSITDVDLGDHIQKEMTVFFCDIRSFTE
                     LSEKMSPRDNFQFLNSYLKRVGPVIRNNGGFIDKYIGDAIMALFSDADSALNASVQLL
                     HEIHEYNYFRIKSNYDPIEVGIGLHTGNLIMGTIGESERMEGTVISDSVNLASRIEGL
                     TKYFGASLLVSGETFRLVRHKEKFHYRILDTVKVKGKSSSVQVVEIFDGFPKEKAALY
                     IKTKSAFESAVAEYQNREFSKCIIEFQKILEINPNDKASQIYLNRAKHFLEFGVPDEW
                     TGIESMTEK"
BASE COUNT          854 a          540 c          603 g          784 t
ORIGIN      
        1 gattttttcc tctgattcgg attccctttt tccgtgagaa tccgacggtt tcatggaata
       61 taatgaaaaa acagtcaaat ttcttttcag tttcggcaat tctgcgtgag ctgaatctgc
      121 ttcatctgaa gtcctgaaag atttgaatcc ttgtcagtgg aaatttccag aagcagattt
      181 atggtttctc cttaaacaag gtatgaagca aaaaagtttt tgaagttcag gaacttcctt
      241 tttccgagca gtccagtctc gattctgaaa tcactcagat aattcacagc tgcttcacgg
      301 ttcatctttt ttttcatttc cgatgtgaaa taattataaa tttcattaca attttccgaa
      361 ttgcagagtt caatgaattt ctctgcagat tccttatgtg aagtttttct gtttcagaag
      421 acaggagctg aaaactgaaa atgaatattt tatattcact tgccttacgc aaatacaata
      481 tgccgtctat atgcaataat tttccgaaag cattgggaaa atctggtccc tactgtaaac
      541 tatccggcga attcattcaa tgccatcaaa tatgaggaat tctgaatgaa tcaaaaaaaa
      601 gaggcacgtc aactattcga ttgaaaataa tgtaagactg agtttcattg taattaatga
      661 aaaatttccg cttttccttc ggatttaaga taggtatggc tctcagcagt ctgtccatgc
      721 ttatttcagc agcgggaatt gcgtttttgt acaattacac atatagggca cttatcgaac
      781 aggttgccaa aaagacagta gaaataggtc agacaagtgc ctatctgttt caggaagagg
      841 aacgggaaga catcaagttt ttgagaagca tttccgaggc gaggatgcag tccctcacgg
      901 aatttgattt agcgatagaa ccaggagaaa ccaaaccgtc cataccggat gatattgcgg
      961 atgaaattat gaaaggggat gagtttcaga aaatagtaca gcttctccgt tccatcaagg
     1021 cggcaagtat ggatcaggtt cagcctctcg gttttctgca gcagattaca ccggagaaac
     1081 cggccaagtt cagatatgtc tatatgcttg ctgagattcc ccaggttccg tccagaaagg
     1141 ttcttaaatt tattgcagac gcagattatg aggcaattga tgaaaacggc gacggcaaaa
     1201 tttccgagga ggaaaaggcc gtccctatcg gcactcttta ttcagtcaac agcagagaaa
     1261 tgatcaatgc atttgacggg aacagttact attccagaga aatttatacg gacaaatggg
     1321 gaaagttcat cggttctatg gttcccatca aggacagtga tggaaaagtt cttgcaattt
     1381 tatgtatgga atatgaggtt tctgaagttc tcaagcagac taagcagatt ctgtacatca
     1441 gcagcgggat tatatttgca agcttcctcc tctcactttt attctcttcc atcgtcgcct
     1501 actggattgc aaagcctatc aatgcactga aagatgcggc agaaaaagta tccaaccaga
     1561 actacaatgt gagcatcacg actgacaggc aggatgagat cggagttctt tccagaacat
     1621 tcggcagtat ggtcagagaa attcaggagt ttaccagaaa actggaagag tccaatatgc
     1681 atcttcaaag actgaaccag tcctacaaaa gatttgtgcc tcaggagttc ctgggatttc
     1741 tgaagaaaaa atcaattacc gatgttgatc tgggagatca tatccagaag gagatgactg
     1801 tatttttctg tgatatccga tccttcactg aactttcaga aaaaatgagt ccaagagaca
     1861 actttcagtt tctgaattcc tatctgaaaa gagtgggacc tgtcatccgc aacaacggcg
     1921 gattcataga caagtatatc ggggatgcga tcatggctct cttctcggat gctgattctg
     1981 cgctgaatgc ttcggttcag cttctccatg aaattcatga atataattat ttcaggataa
     2041 aaagcaatta tgatccgata gaagtcggaa tcggtctgca tacaggaaac ctgattatgg
     2101 gaacaatcgg cgagagtgaa aggatggagg gaaccgtcat ttccgattca gtgaatcttg
     2161 cttccagaat tgaaggtctt acaaagtatt tcggcgcatc gcttctggtc agcggagaaa
     2221 cgttccgtct ggtccgacac aaggaaaagt ttcattatag aattctggat acagtgaagg
     2281 tcaagggaaa gtcctcttct gttcaggtag tggaaatctt tgacggtttt ccgaaagaaa
     2341 aggcagcatt gtacatcaag acaaaatccg catttgaaag cgctgttgct gaatatcaga
     2401 acagagaatt ttccaaatgc attattgagt tccagaagat attggaaatc aatccgaatg
     2461 acaaagcatc ccaaatttat ctgaacagag caaaacactt tctcgagttc ggtgttcctg
     2521 atgagtggac agggattgag tccatgactg agaagtagac actccataaa agcacggttg
     2581 ccgagcggtg tggtgttggt tggttcggct ccgctcatca accaagctgg cgcacataca
     2641 aaaccccaga gtaaataatc gcgaacttgt aagccgctgc aaacctggta tgtacgcaga
     2701 ttaaaaaaat atgatggaga gcacaaattt atactcattt tcataaataa ttgcgccttt
     2761 tccggctgac agacttgatt t
//
LOCUS       DAOQGJ010000316         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22408_length_2778_cov_2.935733, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000316 DAOQGJ010000000
VERSION     DAOQGJ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22408_length_2778_cov_2.935733"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1156)
                     /locus_tag="PK683_06240"
     CDS             complement(296..1156)
                     /locus_tag="PK683_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08083.1"
                     /translation="MKTKISIFIILLTLLNCAGLQKGSSVSGSPEIQKKNGYKADIRK
                     VSVASVYNSEKKTLGDRTDVSIISYFGTVDGRAGEIFGDSFAGGDALDKLASRLKISG
                     FQDSLKNLAESELGNESLRPETARNLSLLIEKGKIDAIVLPFLTGGLDSIVVGKDVKF
                     GLIVFSGKTSSVQIFALGSRIPVSPEDRKLAETSPDRAKAAVNAGAMKNANELLDRLK
                     KEIRNVQDTAASSPQDNKVADNKETKKEDAASVETSEEKKEPEVPKTAFEKMYPPVLF
                     GFTALLWLLL"
     gene            complement(1160..>2778)
                     /locus_tag="PK683_06245"
     CDS             complement(1160..>2778)
                     /locus_tag="PK683_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH08084.1"
                     /translation="LLNSVFGGTHALNFTDRPHKLEWNVNYSQAVRDEPNLQQQVWRR
                     SDPPGTVIGSNGVRYLENYTRLGNNPDGTRFFSRSEDNVRAWNLSYEIPFNQWAGLKS
                     TLKVGAMQSEREKNFRFREFGQKVNTGGGNELYPIPGELLYNPLVFLSGGRTFSERQV
                     EPNAYDASQKLKAVFAQVDMPIIQKWRFVGGFRQEESYQKVQTFVLRDSANPFKKPDT
                     GCTPKNETERLLLINSNVCKADNNGIGELATKDRLPSANFVYEMKEDMNLRFGYSETL
                     TRPDLRELSPFGFTPYFGADRIFGNSDLRRTYIHNYDLRWEWFQKGTDYMAVGVFNKQ
                     LSNPIEMIGQPVAGSISARFTYGNATSAYIRGLEFDYRKEFLNMFRAETNFFFIKSRV
                     DVLPWTTYAFAKAGLLDRNSRAFAYDPTNLSRSLQGQSEFVFNLKLDYFITKTKNTTV
                     GLYYNYFGDRIYAVGANGTPDAIEKGVGLTDAVFQHKADDKWDFKFALRNIMNTRFRI
                     YQHNHLTGRDELFLSYREGLNIALSATMRL"
BASE COUNT          711 a          666 c          571 g          830 t
ORIGIN      
        1 cggaatagtc actgcacgca gtcactagaa gaaaaacgac cggtacagag agaatccgga
       61 aaagcctcat attggaagta tccgaagatt tccggaattg tcaagaaagg tttcattgat
      121 gccggtatgc gctgaattgc cattttcact cagctttcat ctgtctgcgg gattatatac
      181 gggcatattt accggaggcc ctatcggtct gaccgacagg gctgtgaacg cggacaattt
      241 gttccttgct ttccgcatct ctgtaaattt ttacgcctgc acagtttgtc tcaagtcaga
      301 gcagaagcca taacagagcg gtgaatccaa aaaggaccgg aggatacatc ttttcaaaag
      361 ctgtctttgg aacttcaggt tccttttttt cctccgaggt ttcaactgag gctgcatctt
      421 ccttctttgt ttctttatta tcagctactt tattgtcttg aggagaagat gcagcagtat
      481 cctgaacatt tctaatttct tttttcagcc gatcaagaag ttcatttgca tttttcattg
      541 cacccgcatt cacagccgcc tttgccctgt caggacttgt ctctgcgagt tttctgtctt
      601 caggagaaac cggtattctg ctcccaagcg caaagatctg aacagaggat gtttttccgc
      661 tgaatacgat cagtccaaat tttacatctt ttccgaccac aattgaatcc agtccgccag
      721 tgaggaaagg caggacaatg gcatctatct ttcccttctc aatcagaagt gaaagattcc
      781 ttgccgtttc aggacgaaga gattcatttc cgagttcaga ctcagccagg tttttcagag
      841 aatcctgaaa gccggaaatt ttcagtctgg atgcaagttt gtcgagtgca tcccctcccg
      901 caaaactgtc cccaaagatt tctcctgccc ttccgtccac tgttccgaaa taagaaatga
      961 tgctcacatc ggttctgtct ccgagagtct tcttttcgga attgtagact gaggcaactg
     1021 ataccttcct gatatcagcc ttataaccgt tcttcttctg tatctctgga gatcctgata
     1081 cggaagagcc tttctgcaga cctgcacagt tcaataatgt aagcagaatt ataaatatgc
     1141 ttattttagt tttcattgat cataacctca ttgttgcaga aagtgcaatg ttcagaccct
     1201 ctctgtagga aagaaacagc tcgtctctcc ctgtcagatg attgtgctgg tatatcctga
     1261 acctggtgtt cattatgttc cgcagggcaa atttgaaatc ccatttgtca tctgccttgt
     1321 gctgaaacac agcatcagtg agccctactc ctttttcaat cgcatccgga gttccgtttg
     1381 caccgaccgc atagatcctg tctccaaaat aattatagta cagaccgaca gtagtatttt
     1441 tggtctttgt tataaagtaa tccagtttca ggttgaatac aaactctgac tgcccttgga
     1501 gagatctgga aagatttgta gggtcatagg caaacgctct tgagtttctg tccagaagtc
     1561 ccgcctttgc aaaagcatat gttgtccagg ggagaacatc cactctggat ttgatgaaga
     1621 agaaattcgt ttcggcccga aacatattca ggaattcctt ccggtagtca aattccagtc
     1681 ctctgatgta tgcacttgtc gcatttccat aagtgaacct cgcagagatg gaacctgcca
     1741 caggctgacc gatcatttct ataggattgg agagctgctt gttgaataca cccacagcca
     1801 tatagtcggt tcctttctga aaccattccc accttaaatc ataattatga atataagttc
     1861 ttctcagatc tgaatttccg aagatcctgt ctgcaccgaa gtacggagtg aatccgaaag
     1921 gagaaagttc cctgaggtca ggtcttgtga gtgtctcaga gtatccgaaa cgcagattca
     1981 tatcttcttt catttcataa acaaaatttg cagaaggaag tctgtccttg gtcgcaagtt
     2041 cccctatacc gttattatca gccttgcata cattcgaatt gatgagaaga agtctttcgg
     2101 tctcattttt cggtgtacag cctgtatcgg gctttttgaa agggtttgca ctgtcccgaa
     2161 gcacgaaagt ctggaccttc tgataacttt cctcctgacg gaaccctccg acaaatctcc
     2221 atttctgaat aatcggcata tccacctgcg caaaaactgc cttcagcttc tgtgaagcgt
     2281 cgtacgcgtt tggctcgacc tgacgttcag agaaagtcct cccgcctgaa agaaatacta
     2341 aaggattgta aagaagttct cctgggatcg gataaagctc attcccgccg ccagtattga
     2401 ctttctgacc aaattcacga aatcggaaat tcttctctct ttcagactgc attgctccta
     2461 ctttcagggt ggatttgaga ccagcccact gattgaacgg aatctcgtaa gaaagattcc
     2521 aagctcttac attgtcctcg gatctggaaa agaatcttgt cccgtccgga ttgtttccaa
     2581 gtcttgtgta attttccaag tatcttaccc cgtttgaacc tatcactgtg ccaggcggat
     2641 cggatcttct ccaaacctgc tgctgcagat taggctcgtc ccttactgcc tgcgaataat
     2701 tgacattcca ttccagttta tgcggtctgt ctgtaaagtt taaagcatga gtccctccga
     2761 atactgaatt cagaagcg
//
LOCUS       DAOQGJ010000317         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22444_length_2775_cov_3.931250, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000317 DAOQGJ010000000
VERSION     DAOQGJ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22444_length_2775_cov_3.931250"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PK683_06250"
     CDS             <1..249
                     /locus_tag="PK683_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010579369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH08085.1"
                     /translation="ASKHAVAGLAKALREEWKKHRIRISTVYPGAVYTSIWENREGFE
                     KKDMISPEDFGNYLASFSEIPGSAVPEEIHILPLKGIL"
     gene            complement(233..1081)
                     /locus_tag="PK683_06255"
     CDS             complement(233..1081)
                     /locus_tag="PK683_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HNH08086.1"
                     /translation="MIKYAAKGKTLLAFAVISAVMLLFFLEFREVKQKFRERNSELSS
                     SVQKEIDRTFSEIELMLFDINYIINGSAESAQRFSPKITEMLLKKKSERPYIMDILVM
                     DGEKEIIQWTGTGRVPYVKDRDFAAFHTKGESSVLFIGEPKLSRVHTGKWFFGISYAF
                     LDSAGNIVRIVAGLVDLEHISSRLNQLKRERAQTVRAVSAKGFLVSTASEFNERTGDH
                     FYESLEEGIEENVFQIKEIRKSVQGTMFSIVTEKIPLNFFIIRLFVFSLILGILLYLS
                     VSRESL"
     gene            complement(1088..2653)
                     /locus_tag="PK683_06260"
     CDS             complement(1088..2653)
                     /locus_tag="PK683_06260"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH08087.1"
                     /translation="MIKKYILILVSLFAAVLCGPPKPGQGDRTSGSEEKETPMRDSSE
                     VLRDLESAEWSVRSQAAVEAGSRGLKEALPKLRKLFTEDKNPAVRGSSAVALADLKDN
                     ESVPVILKHMKDEPEDSPDVYIEVLKRMGNRQAGKALVPYLNSEMDVIRLLAVDALAT
                     LETKEAGADILRMAEKNGSLEKHKTYAMALGKLGIKEAENYLIGLAEKTEPSPTLAAS
                     ILALGRIKSRKAVPVLSGFLGKPFDKGRENAVDALVMIQDRSCLPLVYPYMSNHDKEI
                     RYTAAKAVAEIPDPASADRVLEILSGKDSVLFGAASYAAGRLKAEKARKKIEEILVKK
                     DVPEREVIAQSLGWIGNKESIPVLISVLQENSGEGRYGAAWSLGIMEAKEAFEDLKKA
                     ASSSDGRLASAALESLGMLKMEESLDFLEKNAKSSKGLALFSIGSISNIPGEKARKIL
                     EEFAKSRELNYFQPAIEALGKRKEKESVPFLISILKDKESSGKLKMTIAALTSITGDK
                     FRSSTEWINKYGN"
BASE COUNT          731 a          672 c          530 g          842 t
ORIGIN      
        1 gcatccaaac atgcagttgc cgggctggca aaagcactca gagaggaatg gaaaaaacac
       61 agaatcagaa taagcacagt ctatccggga gcagtttaca cgtctatctg ggaaaacaga
      121 gaaggatttg aaaagaagga tatgatttcc cctgaagact tcggaaatta ccttgcatcc
      181 ttttctgaaa ttcccggatc agccgttcct gaagagattc atatccttcc tcttaaaggg
      241 attctctaga aacactcaaa taaagcagaa tccctaaaat cagagaaaat acaaaaagcc
      301 tgattataaa aaaatttaat ggaattttct cggttacgat tgaaaacatt gtaccttgta
      361 cggactttct gatttctttg atctgaaaga cattttcctc aattccctct tccagagact
      421 cgtaaaaatg atctcctgtt ctttcattaa attcagatgc agtggatacc agaaagccct
      481 tcgctgagac ggctctcact gtctgcgccc tttctctttt cagctgattc agtcttgaac
      541 tgatatgttc cagatccacg agtcctgcca caatccttac aatatttcca gcagaatcca
      601 gaaatgcata actgattccg aaaaaccatt tcccggtatg caccctcgaa agtttaggtt
      661 ctcctataaa aaggacagag gactctcctt ttgtatggaa tgcagcaaaa tccctgtcct
      721 tcacataagg gactcttcct gttccagtcc attgaatgat ttctttttca ccgtccataa
      781 ccaggatatc cataatgtac ggacgttctg actttttttt cagaagcatt tcagttattt
      841 taggagagaa gcgctgcgca gattcagcag aaccgttgat gatataatta atatcaaaca
      901 gcataagttc tatttctgaa aaagttcggt caatttcctt ctgcactgaa ctgctgagtt
      961 cagaattcct ttcgcggaat ttctgcttta cctctctgaa ttccagaaaa aaaagaagca
     1021 ttactgcgga aataacggca aaagccaaaa gagtctttcc cttggcagca tattttatca
     1081 tgagaaatta attaccgtat ttattgatcc actctgtaga acttctgaac ttgtctcctg
     1141 taatcgaagt cagagcagca attgtcattt tcagcttgcc tgaggattcc ttgtctttca
     1201 ggatggagat cagaaaagga actgattctt tttctttcct ttttccaaga gcctctattg
     1261 caggctgaaa ataattcagc tctctgcttt tggcgaactc ttcgagtatc ttcctggcct
     1321 tttccccggg aatatttgaa atggaaccga ttgaaaacag ggcaagacct ttgctgcttt
     1381 ttgcattttt ctccagaaag tccaggcttt cttccatctt aagcataccc aggctttcaa
     1441 gtgcagctga tgcaagcctt ccgtcactcg atgaagcggc ttttttgaga tcttcaaaag
     1501 cttccttcgc ttccataata ccgagcgacc aggcagctcc gtatcttccc tctccgctgt
     1561 tttcctgcag aacagagatc agcacaggaa tgctttcctt atttcctatc catccgagac
     1621 tctgtgcaat gacttccctt tcaggaacat cttttttaac cagaatctct tcaatttttt
     1681 ttctggcttt ctccgctttc agtcggcctg ctgcatagga agccgctccg aaaagaacag
     1741 aatcctttcc tgaaagtatt tccaagaccc tgtctgcaga agcgggatca ggaatttcag
     1801 caactgcctt tgctgctgta tatcgtattt ctttatcatg atttgacata tacggatata
     1861 ccagaggaag acaggatctg tcctgaatca tgacaagggc atcgacagca ttttctcttc
     1921 ccttgtcgaa gggtttaccc agaaaaccgg aaagaacagg aacagctttt cggcttttga
     1981 ttctcccaag tgccagaatg gaggcggcca gagtagggga gggttccgtt ttttcagcaa
     2041 ggccgatcag ataattttct gcttccttta ttcccagttt tccaagggcc attgcatatg
     2101 tcttatgctt ttccaatgaa ccgttttttt cggccatcct gagtatatca gcaccggctt
     2161 cctttgtttc cagtgtcgcc aaagcatcga ctgccagaag cctgatcaca tccatctcag
     2221 aattcagata cggaaccagt gcttttccgg cctgtctgtt tcccatccgt ttcagcactt
     2281 ctatataaac atcaggactg tcttcgggtt catccttcat atgtttcaaa atgacgggaa
     2341 ctgattcatt atctttcaga tctgccagag ctacagcaga acttccccga actgccggat
     2401 ttttatcctc tgtaaacagt tttcggagtt taggcagtgc ctctttcagc ccccggctgc
     2461 ctgcctccac tgctgcctga gaacggacag accattcggc agattccaga tcgcggagaa
     2521 cctcagaact gtctctcatc ggagtttcct tttcttcgga gcctgaggtc ctgtctccct
     2581 gaccgggttt aggcggtccg cagagcactg cggcaaacag agacactaaa attaaaatat
     2641 atttttttat catgataatc cccggttaaa gacaggtttt tcagcacctg actgtttttt
     2701 tctttttgag aagagttccc cgttctactg tcttttttat atagtccgga gcataaggca
     2761 ccttacattc agtac
//
LOCUS       DAOQGJ010000318         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22448_length_2775_cov_3.318750, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000318 DAOQGJ010000000
VERSION     DAOQGJ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22448_length_2775_cov_3.318750"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..613
                     /locus_tag="PK683_06265"
     CDS             59..613
                     /locus_tag="PK683_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH08088.1"
                     /translation="MNLLKKIGIAAAVIIAIPLIAAIFVKKEYTVLREITINRSKTDV
                     FSYIKLLKNQENYSRWAQMDPKMKKTYQGTDGTAGFISGWESTNKDVGKGEQEIKKIT
                     EGERIDYELRFIEPFQGTEQAFMAVETVSDEQTKVKWGFNGHMAYPMNLMMLFMNFEE
                     MIGKDFDIGLNGLKQILEKKSDEK"
     gene            complement(1121..1321)
                     /locus_tag="PK683_06270"
     CDS             complement(1121..1321)
                     /locus_tag="PK683_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08089.1"
                     /translation="MPDSILNVGAVASVGPFPFTPMVTFQLVFVPSGFVWVIVNTVAG
                     KEAVGAPLMIPVFGSRLRPSGS"
     gene            1584..2546
                     /locus_tag="PK683_06275"
     CDS             1584..2546
                     /locus_tag="PK683_06275"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNH08090.1"
                     /translation="MDCTGTGQDGEFQKGMDRSYTDNGDETISDNVTGLTWHKCVSGT
                     FGQDCSSGTATKYSFENAERFCSESTVGGRKWKLPTMAELSGLVIYKTRPFNSNFLIE
                     TVAWDTGYFPNDTEHSFLGTSERKQDSYGNIMWGKVKFLIIHIQWFFEYDPEAFIRCV
                     EQNSYSIQKSFTDNGDGTIKDNATNLTWQKCVYGQSLDQSCTGQVQSVLWSDAIDYCK
                     NLELAGRQWRLPNKNELLSLFDYRQQQPAVPPPFSDFGSMSNYSTNYGINIADVWSST
                     THATISQYAYPVDFYYGGTGRAWQHGYGKGGAAYFPSAKCVSDP"
BASE COUNT          911 a          534 c          593 g          737 t
ORIGIN      
        1 gaaggccttc gatcaataaa ttactgtcaa gacagacaaa tttcagagga gataaaaaat
       61 gaatttatta aagaaaatcg gcatagcggc tgctgtcata atagcgattc cgctcattgc
      121 tgcgatattt gtaaagaagg aatatactgt tctcagagag attacaataa acagatcaaa
      181 gacggatgtt ttctcctaca tcaaacttct gaaaaatcag gagaactaca gcagatgggc
      241 tcagatggat ccgaaaatga aaaaaacata tcagggaacg gacggcactg caggttttat
      301 atccggatgg gaaagtacaa ataaagatgt aggcaaaggc gaacaggaaa taaagaagat
      361 tactgaaggc gaaagaatag attatgaact gcggttcatt gaaccttttc aaggaacgga
      421 acaggccttt atggctgtag agacagtttc tgatgagcag acaaaagtca agtggggatt
      481 caatggtcat atggcatacc cgatgaatct aatgatgctc tttatgaatt ttgaagaaat
      541 gataggaaag gactttgata ttggactgaa cggactcaag cagattctgg aaaaaaaatc
      601 ggatgaaaaa tgatctgagg attgaataag gatactgttt acaggtcagg atgcggtatt
      661 gtaaaaaacg acgactaatt cctgtgcatc atatcagtat ttgacaaatg aacactaaga
      721 cagataagca gagaatttcg aattttgctt atctgtatca gtctgatacc taaagctgac
      781 cgatgttccg agactctcgt aaactttccc aaaagagtcg ctgattttgg ggatactcat
      841 attcagtaca gataaaattc agaaacagga taatcagtac tattatagta aaaataaaac
      901 catttataaa atgcaggttc aaggaaacat tcctttcatc ataaaagtct cgcaagcttc
      961 cccaaatggt tccgcttgca cttttaggaa caggaaaatc cgcatcaccc ccgatcaaca
     1021 aaacctatcc gacagaggaa accattgttc taaatgtcgg tcagcagctg actctctccc
     1081 cttctctgca cgtagagggc tccacttaca caatcagccc tcagcttccg gaaggtctca
     1141 gcctcgatcc aaaaacaggg atcatcagcg gtgcacccac agcttctttt ccggccacag
     1201 tgttcacaat cacccagaca aaaccggacg gcacaaagac cagctggaaa gtcaccatcg
     1261 gcgtaaatgg aaatgggccg accgatgcaa ctgcaccgac attcagtatt gagtccggca
     1321 aagcatatcc tgttccacag attctgacac tgcagtccag ccccggtgca aagatttatt
     1381 atacaactga cggctcagaa ccgaatgaaa actcaacact ttatactgat aaaatattcc
     1441 ctgtcaggga tttcgcagga aaaagaatca aagcaattgc gatcaatgga gcatccagaa
     1501 gtattgtatc ttctgccgtt tactattatc caattctgaa aacaggtcag actgactgct
     1561 ggatagatgg aatattgaca acggtggatt gcaccggtac aggacaagat ggcgaattcc
     1621 agaaaggaat ggacaggagt tatactgata atggagatga aacaatttca gacaatgtga
     1681 ctgggcttac ctggcataaa tgtgtttctg gaactttcgg tcaggattgc agttctggaa
     1741 cagcaacaaa gtattctttt gagaatgcag aaagattctg ctccgaatcc actgtaggtg
     1801 gacgaaagtg gaaattacct acaatggccg aattgtctgg tctggttata tataagacta
     1861 gaccatttaa ttctaatttt ttaatagaaa cagtggcctg ggatactggc tatttcccaa
     1921 atgatactga gcattcattt ttgggcacgt ctgaaagaaa gcaagatagc tatggaaata
     1981 taatgtgggg aaaagttaaa ttcttaatta ttcatattca atggtttttt gagtatgacc
     2041 ctgaagcgtt tataagatgc gttgagcaaa attcttattc cattcagaaa tctttcactg
     2101 acaatggaga tggtacgatt aaagacaatg ccacaaatct gacttggcaa aaatgtgtat
     2161 atgggcagtc tttggatcag agctgtactg gccaagtcca aagcgttctt tggtcggatg
     2221 caattgatta ttgtaaaaat ttagaactgg ctggaagaca gtggagactt cccaataaaa
     2281 atgaactgtt gagtcttttt gattacagac aacaacagcc agctgtccca cccccattct
     2341 ctgattttgg aagtatgagt aattatagta ccaactacgg aataaacatt gctgatgttt
     2401 ggtcatctac aactcatgcg acaatatctc agtatgctta tccagtcgat ttttattatg
     2461 gaggaacagg aagggcttgg cagcatggct atggaaaggg cggggcagcg tatttccctt
     2521 ccgccaaatg cgtatccgac ccttgaccca ctgaacagaa cagcattttg gaattggatt
     2581 ccgctgtttc aaaatgggaa cagttggaga aggggatact ttccttttcc agaatgctga
     2641 agtgcttcaa atgaataaat tcgttgggaa aattcaggat gaaaatcttt aattttgcca
     2701 acaattctat ttttaattat gactgtattg aattgagctc atctgccccc gcttaatacc
     2761 ttgacaattc tgtct
//
LOCUS       DAOQGJ010000319         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22460_length_2774_cov_3.340566, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000319 DAOQGJ010000000
VERSION     DAOQGJ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22460_length_2774_cov_3.340566"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PK683_06280"
     CDS             <1..772
                     /locus_tag="PK683_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH08091.1"
                     /translation="PKIEEKDNERILLQRESQFLSAEQNFQIASNELAIFLRTESEKN
                     FLPSMEQLPENPFDQIRENESIDVEYGLKLALDKRPEIKRIQAQKEQNILDRELAKNQ
                     MKPGIDFVFAASQDMGPGSETRSKPELEASLVLSIPLATKTQKGKMLSADAKNEKLDA
                     QEKFLRERIEADIRNSFFLLKASLKRADLAKKEKSLAKELEAAERNRFQIGEGTLILV
                     NIREQTSAEALNREVDALAERNRAWALYRTSTGEWTFE"
     gene            complement(775..1290)
                     /locus_tag="PK683_06285"
     CDS             complement(775..1290)
                     /locus_tag="PK683_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08092.1"
                     /translation="MAAQEIYKFKYIIMLVFITVTQNPFSAAELKEKPSKETFKFTES
                     ETRKNVLKFHKAGLYISEQCVRRTGKVYVKRECGAWKLIEKLESGDYRKYFTPDKIWP
                     DLSRPKSVSIFLCIRSGGFLTTETNEYGRYSSAETVDFCQYKDDSLLALKSIERNLEK
                     APPLLPLPKYP"
     gene            complement(1268..>2774)
                     /locus_tag="PK683_06290"
     CDS             complement(1268..>2774)
                     /locus_tag="PK683_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH08093.1"
                     /translation="KTKSLEIRLILLKQSPDSMFLYNLFLLFSLRETAYLFYIGFTGS
                     YTMLQAVGHGHAFQYLWPDSPEWTNTAFAFFLGLTIFFANLFADNFLKIKNHLSYLSH
                     LLRFSALSGLLICLFSFYVSTGIINQTALTIGLFSVFLILAAGIVASQKKFRPARFFL
                     LAWGMFLFFAAVTVLSMFGFIPVSFLTLYGIQIGSAAETILLSFALADKINLIKKENQ
                     DAIDRTIQLQEEATIELESKVQDRTKRIQDFYEHIQKDISMASIIQEKIIPRNFTHPS
                     LKVNSIFLPLDVIGGDIFDIYEISPGKVRFFLADATGHGVQAALITMLIKSEYDGLKY
                     FISDPGDLLFSLQIRFTEGYKGMRIYFSAIVLDIDLNESRIYYSSAGHPPQAVQREDG
                     IIKLHRTGAIIGIQSTRSYETKETPVSKGDRVFLFTDGLLEVFDEKNSQLGEEGFLRF
                     ISEHKNENVALQMHNCLLDMRHFMGIKEPTDDITFLGIEIGDYSNGSSGNL"
BASE COUNT          811 a          564 c          630 g          769 t
ORIGIN      
        1 tcctaagatt gaggaaaagg acaatgaaag aattcttctt cagagagaat ctcagtttct
       61 ttccgcggag cagaactttc agattgcatc caatgaactc gctatttttc tccggacaga
      121 atctgaaaag aactttcttc cttcaatgga acagcttccg gagaatcctt tcgatcagat
      181 ccgcgaaaat gaatcgatcg acgtcgaata cggactgaag ctggcgctcg ataagagacc
      241 tgagattaag aggattcagg ctcagaagga acagaatatt ctggacagag agcttgcgaa
      301 gaaccagatg aagcccggga tagactttgt ttttgctgcc tcacaggata tgggaccagg
      361 aagtgaaacc agatcaaagc ccgagcttga ggcatcactg gtactgagca ttcctcttgc
      421 aacaaaaact cagaaaggga aaatgctttc tgccgatgca aaaaatgaga aactggatgc
      481 acaggaaaaa ttccttcggg aaaggattga agcggacatt agaaattcat tttttctgct
      541 gaaggcatca ttaaaaagag cagaccttgc caaaaaggaa aagtctctgg cgaaggagct
      601 tgaggcagcg gaaagaaaca gatttcaaat cggtgaagga actctgattc tggtaaatat
      661 cagggagcag acctcagctg aggcactgaa cagagaagtg gatgcactgg cagaaagaaa
      721 cagggcatgg gcactttaca gaacttcgac gggagaatgg acctttgaat agagtcaggg
      781 atatttaggc agaggcagaa gagggggagc tttctccagg tttctttcta ttgactttaa
      841 tgcaaggagg gaatcatcct tgtactgaca gaaatcaact gtctccgcac tggaatatcg
      901 gccatactca tttgtttccg ttgtcagaaa acctcctgaa cggatacata gaaaaatgct
      961 gacagatttc ggtctggaga ggtcaggcca gattttgtca ggagtaaagt attttcggta
     1021 gtctccgctt tccagttttt caatcagttt ccaggctccg cattctctct ttacatagac
     1081 ttttcctgtg cgtcttacgc actgttcact gatgtaaagt cctgccttgt gaaatttcag
     1141 cacgtttttt ctagtctctg attccgtgaa tttaaaagtt tccttcgaag gtttttcctt
     1201 gagttcggcg gcagaaaaag gattctgggt cacagtgata aaaacaagca taataatgta
     1261 tttaaattta taaatttcct gagctgccat tgctgtagtc tcctatctca attcctaaaa
     1321 aagtaatgtc gtctgtaggc tctttgattc ccataaaatg tctcatatcc agaaggcagt
     1381 tgtgcatctg cagtgctaca ttttcgttct tatgctcgga aatgaagcgc agaaatcctt
     1441 cttcccccag ctgtgaattt ttttcatcaa atacttccag cagtccgtca gtaaaaagaa
     1501 agaccctgtc tcctttgctg acaggagttt cctttgtttc ataggagcgg gtgctctgta
     1561 ttcctataat ggctcctgtt ctgtgaagtt taatgatccc gtcctctctc tgaacagcct
     1621 gaggaggatg ccctgctgac gaataataaa tcctgctttc attcagatct atgtcaagaa
     1681 caattgcgga aaagtaaatt ctcatcccct tgtatccctc tgtaaaacgg atctgaaggg
     1741 agaacagtaa atcgccggga tccgaaataa aatatttcag tccgtcatac tcgcttttga
     1801 tgagcattgt aatcagtgca gcctgcactc cgtgtcctgt tgcatcggcg agaaaaaagc
     1861 ggacttttcc ggggctgatt tcatatatat caaatatatc gcccccgatc acgtcaaggg
     1921 gcaggaaaat ggaatttact ttcagtgaag gatgtgtaaa atttctggga atgatctttt
     1981 cctggatgat gctggccata ctgatgtctt tctgtatgtg ctcatagaaa tcctggattc
     2041 ttttggttct gtcctgcacc ttgctttcaa gttcaattgt cgcttcttcc tgaagctgaa
     2101 ttgttctgtc tattgcgtcc tgattttcct ttttgatcag attgattttg tctgcaagtg
     2161 caaatgaaag aagaatagtt tctgccgcgg atccgatctg tatcccgtac aatgtcagaa
     2221 atgaaacagg gatgaatccg aacatactga gtacagtgac tgcagcaaag aaaagaaaca
     2281 ttccccaggc aagaagaaag aatctcgccg gtctgaattt cttctgtgaa gccacaatgc
     2341 ctgccgcaag gatcaggaat acgctgaaca gaccgatggt cagtgctgtc tggttgatga
     2401 ttccggtcga gacataaaag gaaaagaggc agatcaaaag tcctgagagt gcagaaaacc
     2461 tcagcagatg gcttagatac gataaatgat tttttatttt taaaaaatta tcagcaaaaa
     2521 gattggcaaa aaaaatcgtc agccctaaga aaaatgcgaa tgcagtattt gtccattcgg
     2581 gactgtccgg ccagagatac tggaatgcat gaccgtgacc tactgcctgg agcattgtat
     2641 atgatcctgt gaatccgatg taaaacaggt aagcggtttc tctgagcgag aacagaagaa
     2701 ataggttgta taaaaacatg ctgtctgggc tttgcttcag aaggattaaa cggatttcca
     2761 atgacttcgt cttt
//
LOCUS       DAOQGJ010000320         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22482_length_2773_cov_3.005151, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000320 DAOQGJ010000000
VERSION     DAOQGJ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22482_length_2773_cov_3.005151"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="PK683_06295"
     CDS             <1..1423
                     /locus_tag="PK683_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08094.1"
                     /translation="LVLFPLIQSQISSGSFHLFRLEDILSAIQKNKILFISALILALF
                     SFFQFHHTSLLSEEAARKHWPLDYYFSFSSKFRSFAHLLYGNWFSYFPDFGNSEFSAY
                     SSATVCIFSLFGILYAPKDRFWFFYFFLLCLGILSANADNPVYRFMFSNVSGFGNFRA
                     PGRYILFVQISLIYFFCRSLYIGIYKNEYYRKEIRQFSVLISILLIPVLLFPFYKNSS
                     RLPFSAEGFQKAFPDILIISLSVFIYLLLILRPSGEKILSFLAALIMMDCVLRTENTD
                     KVKNLPKYAIEMKRTIRGSLRETLTDNFDLLSRNYGTSGYLTLEKLDTFKFKEKYYQL
                     TESSKEKIRQVLSASGTPADCGLAYIPEKIYYNDFEFYSSISSEKFKCSIYINSENKQ
                     EKALVSSYERLLFTEISGRKFTLSSDKKLKGYIFTAFTYSDLWEARDREGNLLKVQKA
                     LGNFMTVYAENETDLISFSYRFF"
     gene            1801..2514
                     /locus_tag="PK683_06300"
     CDS             1801..2514
                     /locus_tag="PK683_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH08095.1"
                     /translation="MFRTAGFYLYFFIRGFFSFWGRWKANRVQLNTVEETDWEKCKVP
                     RQWARSLVNIVGGKITVNGTENLPKGPVVFVSNHQGNFDIPVLIGFIPKPFGFISKME
                     VMKIPVVGSWMKIMNCVFMDRSSRAKSTQAITAGVEILKKGHSLVIFPEGTRSKGGPV
                     QAFKAGSVRLAIDAEVPIVPIAINGTANIMEKNKYIMKPAEVTVTILPPIPAEKVKSS
                     DSKTLAEELKSLIEAKVKK"
BASE COUNT          846 a          534 c          548 g          845 t
ORIGIN      
        1 tctggttctt ttcccgctga tacagtccca aatttcctcc ggaagctttc atttattcag
       61 actggaggat atcctttcag ccattcagaa aaataaaatt cttttcataa gtgcactgat
      121 tctcgctcta ttttcatttt tccagttcca tcatacgagt cttctttctg aggaagccgc
      181 aagaaaacac tggcctctcg attactattt cagcttttct tccaagttca gaagttttgc
      241 tcatctgctt tatggaaact ggttcagcta ttttcccgat ttcggaaaca gcgaattctc
      301 cgcatactct tcagccacag tatgcatttt ttctcttttt ggaatccttt atgctccaaa
      361 ggacaggttc tggttctttt atttctttct tctctgtctg ggaatccttt ctgcaaatgc
      421 agacaaccca gtttacagat ttatgttttc gaatgtctcc ggtttcggaa atttccgggc
      481 tcctgggcgc tacattctgt ttgtgcagat ttccttgatt tattttttct gcagatcgct
      541 ttatatcgga atttataaaa acgaatacta cagaaaggag atccggcagt tttcagttct
      601 gatttcgata ctgctcattc cggttctgct ttttcctttt tataaaaatt catccagact
      661 tcctttcagt gcggaaggat ttcagaaagc atttccggac attctgatca tatcactttc
      721 agtgtttatc tatctcctgc ttatcctgag gccgtccgga gagaagatcc tttcctttct
      781 ggctgcattg attatgatgg actgtgttct gcgcacagaa aacacagaca aagtaaaaaa
      841 ccttcccaag tatgcaattg aaatgaaaag aacaataaga ggaagtctga gagaaactct
      901 gactgacaat tttgatctgc tttcccgtaa ctacggcaca tccgggtatc tgactctcga
      961 aaaactggac acttttaaat tcaaggaaaa atactatcag ctgactgaaa gctcaaagga
     1021 aaaaatcaga caggttctgt ccgcctccgg aacccctgca gactgcggtc tggcttatat
     1081 ccctgaaaaa atatattata atgattttga attctactca tccatttcat cggaaaaatt
     1141 caaatgcagc atatatataa attctgaaaa taagcaggaa aaggctctgg tatcttctta
     1201 tgaaagactt cttttcactg aaatatccgg cagaaaattt actctttctt cagataaaaa
     1261 acttaaaggt tacatattta cagcatttac ttattcggat ttatgggagg caagagacag
     1321 ggaaggaaac cttctcaaag tgcaaaaagc attaggaaat ttcatgactg tctatgcaga
     1381 aaatgaaaca gacctgatca gtttttcgta cagatttttc tgaatatatg atattgcaaa
     1441 aatgaatata ggtacagcct ggcagttttt cagattttga tgaatcagaa aaaacagcgg
     1501 caggcatctt cataaagctt tgcgagggca aaggcgtaag gcggtctgag caggagccgc
     1561 tgctatgctc tgcctcacgg ccattggaaa tcggggaaat aagctgacct gtcagatttg
     1621 acataaatta atctttactt tttcttaatt acatctctgt tttgatatcg cagagcagtc
     1681 tgctggaagt gactgcgaac agtatccata aaatgccgtt tcaatctgca aatgagttga
     1741 cagtaaagag acagagtttt taatgcttag cattgagaat aatttctctg aggataatat
     1801 atgttcagga cagccggttt ttatttatac ttttttatca gaggtttttt cagcttctgg
     1861 ggacgctgga aagcaaaccg tgttcagctg aatacagtag aggaaactga ctgggaaaaa
     1921 tgcaaggtac caagacaatg ggcaagatca ttggtaaata ttgtaggcgg aaaaattact
     1981 gtcaatggga ctgaaaatct gcctaagggg cctgttgttt ttgtttccaa tcaccaggga
     2041 aactttgata tacctgttct gatcggtttc atacccaaac ctttcggatt catttccaaa
     2101 atggaagtaa tgaaaattcc ggtagtagga agctggatga aaattatgaa ctgtgttttt
     2161 atggacagat ccagcagagc caagtccact caggcgatca cagccggtgt agagatattg
     2221 aaaaaaggtc attcactggt aatttttccg gaaggaacca gaagcaaggg agggccggtt
     2281 caggccttca aggcaggaag cgtaaggctt gcgattgatg cagaggttcc tatcgttcca
     2341 atcgcaatca atggaaccgc aaatataatg gaaaaaaata aatatataat gaaaccagcc
     2401 gaagtgactg ttacgattct tcctccgatt cctgcagaga aggtcaagtc ttcagacagc
     2461 aaaaccttgg cagaggaatt aaagtccttg atagaagcaa aggtgaaaaa gtaggctgag
     2521 tttcttcaac ggccagctct atcggaaata tatctcttta tattttcatt tgagtactga
     2581 atcattacag ttaatgataa aaatagacaa gtcagcatta aattcttgtc tattatcagt
     2641 gtatgatata tatctgcagt attttttcca gctaaagata atacggaaac actgaatata
     2701 taaaatacgc cgatgtaaaa aaaggaagca gattttatac ggttttcaga caggaacaga
     2761 gcaattacaa tca
//
LOCUS       DAOQGJ010000321         2771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22507_length_2771_cov_3.987113, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000321 DAOQGJ010000000
VERSION     DAOQGJ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22507_length_2771_cov_3.987113"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PK683_06305"
     CDS             complement(<1..598)
                     /locus_tag="PK683_06305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNH08096.1"
                     /translation="MKILPKIRENSVDTVFADPPFNIGKIYRKGTNDSRPDYDYIDWS
                     KQWIRECVRVLKPGGSIFLYNLPKWNIIFGNYLLELGMDFRHWIAIELNMVLPIQGRL
                     YPSHYSLLYYSKGKPNVFRKIRTPIEKCRHCGGEIKDYGGHRKAMNPNGVNLKDVWTD
                     IPPVRHRKFKSKNRNANALSTKITDRIVEMSTLPGDIVL"
     gene            826..1254
                     /gene="yiaA"
                     /locus_tag="PK683_06310"
     CDS             826..1254
                     /gene="yiaA"
                     /locus_tag="PK683_06310"
                     /inference="COORDINATES: protein motif:HMM:NF008482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HNH08097.1"
                     /translation="MQSIPQKPSSAFIAISWLSLFVGMITFIIGIWSTKMMLNEKGFY
                     LTVLLYGLFSSVSLQKTVRDQLEGLFVTGIYVGLCWFSVGASLLLLLIGLWNAELALS
                     EKGFLGISYVLSLYAAVAVQKNVRDSRLADMQAGKFSDKE"
     gene            complement(1269..2198)
                     /locus_tag="PK683_06315"
     CDS             complement(1269..2198)
                     /locus_tag="PK683_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNH08098.1"
                     /translation="MKLLCFLLLSFSVPIISENNDKILSNRENLRRHIVRLTSTESFR
                     NFENIRTLNSAGDYIFDELKKTGLSPERQRYSVRSKTVFNVNAEIKKSDSLKTILIGA
                     HYDAAGNQDGADDNASGTAGLLELARILKSSEKSIHYNIIFSAFTLEEPPFFRTEQMG
                     SYIHARSLFQSGRKIDLMISLEMIGYYSDQENSQEYPSFLMKPFYPSKGNFLAGTGRG
                     KEKVWLKKILKAYEKYTDLPFEYLSTDLNISGTDFSDHLNYWKFNYPAIMITDTSFLR
                     NPNYHEQTDTVDTLDFSRMKKAVDGLYFFLSME"
     gene            complement(2195..>2771)
                     /locus_tag="PK683_06320"
     CDS             complement(2195..>2771)
                     /locus_tag="PK683_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08099.1"
                     /translation="QNEFMEDLPESAAHFTRLAKGGSMSHVLRPYPDQFFAPWFSLTK
                     HLKEENFPPKIYESDMLLSSRFDSAFKGTHGADPDNFMEEFQYAFLSWIVSADTKDKN
                     ERAFLRWRHLLLSIYNAGDRNISDYPELFERIAECMLVQFDRLADDFFGEGSFLTEQI
                     DYLLEDMAECRDPEVASKSSELRLYINMRKK"
BASE COUNT          777 a          556 c          538 g          900 t
ORIGIN      
        1 cgagtacaat atcaccaggc agagtgctca tctcgacaat tctgtctgta atctttgtgg
       61 aaagagcgtt tgcattacga tttttagatt taaattttcg atgacgtacc ggagggatat
      121 cagtccatac atctttaaga tttacgccgt tcggattcat cgcctttctg tgaccgccat
      181 aatcttttat ttcaccgccg caatgcctgc acttttcgat gggtgttctt atttttctga
      241 atacattagg ctttcctttt gaataataaa gcaagctgta atgacttgga tataatcttc
      301 cttgaattgg cagaaccata ttcagttcaa tagctatcca atgtcggaaa tccattccaa
      361 gttccagtaa ataatttcca aatatgatat tccatttcgg aagattgtat aagaaaatag
      421 atccacctgg ttttaagact cttacacact cccgtatcca ttgtttcgac caatcaatgt
      481 agtcatagtc tggcctgctg tcatttgttc ctttcctgta tattttccca atgttaaatg
      541 gaggatcagc aaaaacagta tctaccgaat tttcgcggat ttttggtaga atcttcatac
      601 aatcctgatc aaataatata cctcggtccg attcatagta aggctcaatg ctctcacaga
      661 atattcctga ccagttaatt tttttgacag ggtaaactag tttcagctgt tttagagaag
      721 acatagtgtc agtattcagt tttttagtat atactcaaga gatttttagg tcaagtccgt
      781 tcgaatattc ttgacaactc taatattata tatttcttaa acgttatgca gtcaatacct
      841 caaaaacctt cctcagcatt catagccatt tcctggctgt cacttttcgt cggtatgatt
      901 acgtttatca ttggaatatg gagtaccaaa atgatgctca atgaaaaagg tttctatctt
      961 actgtccttt tatatggatt attttcttca gtgtccctgc agaagactgt aagagatcag
     1021 cttgaaggac tttttgtcac tgggatttat gtcggtctat gctggttttc agtaggtgct
     1081 tctctgcttc ttcttttgat cggactttgg aatgcagaac ttgccttaag tgaaaaaggc
     1141 tttttaggaa tttcttatgt cctgagtctg tatgctgcag tggcagtgca gaaaaatgtc
     1201 cgtgattcca gactagctga tatgcaagct ggtaaattct ctgacaaaga ataatcggga
     1261 aggctgcatt attccatact gaggaaaaaa tacagcccat ccactgcctt cttcattctg
     1321 gaaaaatcca gagtgtcaac agtgtctgtc tgttcgtgat aattcggatt tctaaggaaa
     1381 gaggtatcag taatcataat ggccggataa ttaaatttcc agtagtttaa atgatcagaa
     1441 aaatcggttc cggaaatatt caaatcagtt gaaagatatt caaaaggaag atctgtgtat
     1501 ttttcataag cttttaatat ctttttgagc cagactttct cctttcctct tcctgttcca
     1561 gccagaaaat ttccttttga aggatagaac ggtttcatca gaaaagaagg atattcctga
     1621 gagttctcct gatcggaata ataaccgatc atttccaggg atatcatcag atcaattttt
     1681 cttccggact gaaaaaggga ccgggcgtga atgtaactgc ccatctgctc tgttctgaaa
     1741 aaaggaggtt cctccagcgt aaatgcagaa aaaatgatat tataatgaat tgatttttcg
     1801 gatgatttta gaatccttgc aagctccagc agtcctgcgg taccggaagc attgtcgtct
     1861 gcaccgtcct gatttcccgc agcatcatag tgtgcaccga tcagaattgt cttaagtgaa
     1921 tcggattttt tgatctctgc atttacgttg aatactgttt tgcttcgtac agaatatctc
     1981 tgcctttcgg ggctgagtcc agtttttttc agttcatcaa agatgtaatc gcctgcactg
     2041 ttcagtgtcc ggatattttc aaagtttctg aagctttccg tggatgtaag tcttacgata
     2101 tgccgccgga ggttttccct gtttgataat attttatcgt tattttctga tataatcggg
     2161 actgaaaagg agaggagaag aaagcagagc agtttcactt ctttctcata ttgatgtaaa
     2221 gacgcagttc ggatgacttg gatgccactt ccggatccct gcattctgcc atatcctcca
     2281 gaagataatc aatctgttca gtgagaaatg aaccctcacc gaaaaaatca tctgcgagcc
     2341 tgtcaaactg tacgagcata cattcggcga ttctttcaaa aagttcagga tagtctgaaa
     2401 tattcctgtc acctgcattg taaatggaaa gcagcagatg tctccatctg aggaacgctc
     2461 tttcgttctt gtcttttgta tctgcactta caatccagct cagaaaagca tactgaaact
     2521 cttccataaa attgtccggg tcggcaccgt gagtaccctt aaatgcagaa tcgaatcgcg
     2581 aacttaaaag catatcagac tcatatattt tcgggggaaa attttcctct ttcagatgtt
     2641 ttgtcagaga aaaccaagga gcaaaaaact gatccggata aggccgcaga acgtgagaca
     2701 tcgacccgcc ttttgcaagt ctggtaaaat gggcagccga ttcaggaagg tcttccataa
     2761 attcattctg t
//
LOCUS       DAOQGJ010000322         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22536_length_2769_cov_2.855932, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000322 DAOQGJ010000000
VERSION     DAOQGJ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22536_length_2769_cov_2.855932"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PK683_06325"
     CDS             complement(<1..339)
                     /locus_tag="PK683_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08100.1"
                     /translation="MEFLHNILHIRKSVYAGNEYDIELHSLNDLKTYSSAEAHITLYP
                     YSRKIHSDHIGFFPFEDYVRDIQSNYHSAYSRSERGFHRIFGFLLGFIIVMFFYIFKP
                     SELYSLEGVVS"
     gene            complement(339..2378)
                     /locus_tag="PK683_06330"
     CDS             complement(339..2378)
                     /locus_tag="PK683_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08101.1"
                     /translation="MFLNRDDLSSVLLKFQIRYFFIILLFAVPIFSENRDGRPVKSAA
                     ELIREGDSFYREKNYRRALGSYKAALEVNSGSNSALLGYAKSSLQVGSVEDARTAFKI
                     VVDKDHSNNDALAGYADALSRSDKHADALKVLEKRMETDPYNPGLLMIRAEILLRMGK
                     NDLALKRLEDAKGKIQRNYDFDLLLAKAYIANKRFQPASEIIHNLTRQQPENPENFTE
                     KARLNLELAAREENGEKVSALVREALGLLETSVSLDKTGAAPKRLLIRTHLWLGNRSE
                     ALRITEELLEDYPSDSELLYFHSYLSTELGHQDKAVQSFSTLMKTDDLNSIARFSAEE
                     FAKSHLQERHSLRVKLGRYHYEEYLRISREFLYDTARFHISRAKELIPENKFLKSALL
                     DYYYKFGYRSNLIRMLLKLRQDDPDDIKINNRLEAVLKSMRESLSYLEGFLDKEGELI
                     TGARNPVEIFLFDLKPLKFLPDYPDAGNLLNIAIRHSLQLKSSVKPVSGKDEEVIRNA
                     LASSREKAKYTGGIYYYPEILEKLNIGRKKESYIRYIGYGEYETDHNVMKINYHIYDR
                     QSGKVINTSRIISKGRNMMAEVSSRIAVNTASAIQPEGRIVKVKPDSFIINLGFRDGI
                     KKGSKIRIFENDREIGTAPVENLDEYISEIRPKNSSWRENVGLGFRVRPALPEKK"
     gene            complement(2359..>2769)
                     /locus_tag="PK683_06335"
     CDS             complement(2359..>2769)
                     /locus_tag="PK683_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNH08102.1"
                     /translation="SDLTPELGPRAVILLVSGDSLSNAFNQYSFQRNIQFAVSHSIPV
                     YVLALNAETEMAEMYKDLAERTGGRFIRVPGAKEENQLYNMIRSAKDKRYIISFKSKV
                     NHDLAGRYIDFEVSVLYKDITGKAQGGYFVPEQR"
BASE COUNT          762 a          624 c          543 g          840 t
ORIGIN      
        1 agagaccaca ccttccagag aatagagttc agaaggcttg aatatgtaaa aaaacattac
       61 aatgatgaat ccgagcagga agccgaatat acggtgaaag ccccgctcag atctggaata
      121 ggcagaatga taattggact gaatatcccg aacatagtct tcaaacggaa aaaaacctat
      181 atgatccgaa tgaatttttc tgctgtaagg atacagtgta atatgagctt ctgctgatga
      241 gtaggttttg agatcattca gactgtgaag ttcaatatca tattcattcc ccgcatatac
      301 cgatttcctg atatgcagta tattgtgaag gaactccact acttcttttc cggcagtgcc
      361 ggtctcactc tgaaaccgag acctacattt tctctccagg atgaattttt ggggcggatc
      421 tcagaaatat actcatcaag attttccacc ggtgcagtac cgatctccct gtcattttca
      481 aagatcctga tcttggagcc ttttttgatc ccgtccctga atcccagatt gatgatgaaa
      541 ctgtctggct ttacctttac aattctgcct tccggctgaa ttgcagaagc agtattcaca
      601 gcaatccgtg aagatacctc agccatcata tttcttccct tcgaaataat tctggatgta
      661 ttgattactt tcccgctttg acgatcatat atatgataat taattttcat tacattatga
      721 tctgtttcat attcaccgta tccgatatag cgtatatagc tttctttttt tcttccaatg
      781 ttcagttttt cgagaatctc gggataataa taaatgcccc cggtatattt tgccttttct
      841 ctcgaggaag ctaaagcatt cctaatcact tcctcatcct ttccggaaac aggctttact
      901 gaagacttca gctgaagaga atgtctgatc gcaatgttca ggagattgcc tgcatcagga
      961 tagtcgggaa gaaacttcag aggtttcaga tcgaacagaa aaatctcgac aggattcctt
     1021 gcaccggtaa tcagttcccc ttccttgtcc aaaaatcctt ccagatagga aagactttcc
     1081 ctcattgatt tcagaactgc ttccagcctg ttgttgattt ttatgtcgtc aggatcgtcc
     1141 tgcctcagtt tcagaagcat tctgatcaga ttggatctgt atccgaactt atagtaatag
     1201 tccaaaagtg cagacttaag aaatttattt tcaggaatca gttcctttgc cctggatata
     1261 tgaaatctcg cagtgtcata aagaaactct ctgcttatcc tgagatattc ctcataatga
     1321 taacgcccaa gttttactct gagagaatgt ctttcctgca gatgactctt tgcaaattct
     1381 tctgcagaga accttgcaat ggaattcaga tcatctgtct tcatcagtgt actgaaactc
     1441 tgcacagcct tgtcctgatg cccaagctct gtggaaagat aagaatgaaa atacagcagt
     1501 tctgaatcag aaggataatc ttccaaaagt tcttctgtaa tgcggagggc ctcggatctg
     1561 tttccgagcc agagatgtgt tctgatcaga agacgtttgg gtgccgctcc ggtcttatcc
     1621 agtgaaacag aagtctcaag cagtcccagc gcttccctga caagtgcaga aactttttca
     1681 ccgttctctt ctctggctgc aagttcgaga ttgagtctgg ctttttcggt aaaattttca
     1741 ggattttccg gctgctgtct tgtaagattg tgaatgatct cggatgcagg ctgaaatctt
     1801 ttattggcga tatatgcctt tgcgagaaga agatcaaaat cataatttct ctgaatcttt
     1861 ccttttgcat cttcaagtct cttgagggcc agatcatttt tccccattct cagaagaatt
     1921 tctgcccgta tcataagcag tccaggattg tacggatctg tttccattct tttctcaaga
     1981 accttcagag catctgcatg tttgtctgaa cgggatagtg cgtcagcata tccggcaagc
     2041 gcatcgttgt tggaatgatc cttgtccact acaatcttga atgcggtcct ggcatcctcc
     2101 acagaaccga cctgcagaga agactttgca tagccgagca gagcagaatt ggagccggaa
     2161 ttcacttcaa gagcagcctt gtagcttccc agagcccttc tgtaattttt ttcacggtag
     2221 aaagaatcac cttcccgaat cagctcagcc gccgatttta caggtctgcc gtccctgttt
     2281 tcagaaaaaa tcgggacagc gaacagcaat ataatgaaaa aatatctgat ttgaaatttc
     2341 agaagaactg aagataaatc atctctgttc aggaacaaaa tatccccctt gcgccttccc
     2401 ggtaatgtcc ttgtacagaa cagaaacttc aaagtctatg tatctgcctg caagatcatg
     2461 atttaccttg cttttgaatg atataatata ccgtttgtcc ttcgcagaac gtatcatatt
     2521 gtacagctga ttttcctctt ttgcaccggg gacacggata aaccttccgc cggttctttc
     2581 cgccagatcc ttgtacattt ccgccatctc ggtttctgca ttcagagcaa gtacatagac
     2641 tggaatggaa tgagaaactg caaactgtat gtttctctga aaagaatact gattgaatgc
     2701 atttgaaaga gaatcacctg aaacaagaag aatgactgcc ctcggtccaa gttcaggagt
     2761 cagatcact
//
LOCUS       DAOQGJ010000323         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22608_length_2763_cov_2.396603, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000323 DAOQGJ010000000
VERSION     DAOQGJ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22608_length_2763_cov_2.396603"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK683_06340"
     CDS             complement(<1..370)
                     /locus_tag="PK683_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08103.1"
                     /translation="MNDSGTTGSAADYLIRAVPSHWKLNKTSLFESVFRCEFHIPNSF
                     IIIPFTVFLKVRPSENRKDRVFIKKLNADEFSRLENPVFITAYVTEEKQGFWEWKHSA
                     QISGGKENTIMVSVPRSKSLQ"
     gene            complement(536..2578)
                     /locus_tag="PK683_06345"
     CDS             complement(536..2578)
                     /locus_tag="PK683_06345"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNH08104.1"
                     /translation="MKILPDILNNPRAHGFLRAAAVSPKVRVGDPDTNTSAILAAAED
                     AAGKGCEIILFPELCITGYTCADLFSQGTLLKKAEDCLLHLKDSLPKITAIVGVPLEI
                     EGGVWNCAAVLQDREIRGIVPKAFLPNSREFYEGRWFTRQSPSVPVRNVNIRSLKNIP
                     FGSLLFRQGEVIFGIEICEDLWVSDPPSSRLYLQGADIIFNPSASPELAGKSGYRKDL
                     VSGQSARCIGSYVYASAGPSESTTDLVFSGHCIIAEYGTVLDEKRPRLLETDPEKMNS
                     SIHCIAIADIDWKKTRHERIQTSSFQEQARSVPRTEVIELSESGIALNAESEKLLRSV
                     DPFPFAPKGEKSLSERSIEIFDLLTHGLVTRLVHTQSKHIVLGLSGGLDSTLALLVAR
                     RAMTHLGWDISRVVCISMPGMGTSSRTKNNSENLAKVMGTSFLEISIVEAVQKHFQDI
                     GLDPNDRSVTFENCQARERTQILMDYANKVGGFVLGTGDLSEMALGWSTYNGDHMSMY
                     AVNSSIPKTLVKHLVSWASANLVNDFGFKKDDSAETKAREVLLDIVGTPISPELLPPD
                     EKGEITQKTEESVGKYDYIDFFLYHTLRWGASPSKIVYLAMLAFGENHLNEIIDTLSN
                     FYKRFFSQQFKRNCTPDGPKVGSVTLSPRGDWKMPSDAHASLWLEELSELKKILGK"
BASE COUNT          730 a          611 c          608 g          814 t
ORIGIN      
        1 cctgaagact ttttgatctg ggaactgaga ccatgattgt gttctctttt ccccccgaga
       61 tctgtgcaga atgcttccat tcccagaatc cctgtttttc ctctgtgaca tacgctgtaa
      121 tgaaaacggg attttccagc ctgctgaatt catctgcatt gagcttcttg ataaataccc
      181 tgtcttttct gttttcagaa ggtcttacct tgaggaatac agtaaacgga ataataataa
      241 aactgttagg gatatgaaat tcacaccgaa atacggactc gaataaggac gtcttattca
      301 gtttccagtg agagggaacg gctcttatta aataatcagc cgcagatcct gttgtaccgc
      361 tgtcattcat attcttctcc tgaaaacagt cgagactgca ccggcagctg cattttactt
      421 atatcgggag ataatgcaaa tttcattaaa aatctgaata tcccattttg attttgaaga
      481 ttcgcctgaa tgatagacag gaaaagtttc agtttcgaat aattcaagtg aattctcact
      541 tacccaggat tttcttcagt tccgaaagtt cttcgagcca gagactggca tgagcatcac
      601 tcggcatttt ccagtcgcct ctcggggaaa gtgtcactga tcccactttc ggcccgtcgg
      661 gagtgcagtt tcttttgaac tgctgtgaaa agaacctttt gtaaaaatta gaaagtgtgt
      721 ctatgatttc gttcagatga ttctctccga atgcaagcat tgcaaggtag acgattttcg
      781 acggtgatgc tccccagcgg agagtatggt aaaggaaaaa atctatataa tcgtatttcc
      841 ctacggattc ttccgtcttc tgagtgatct ctcctttttc atcaggagga agaagctcgg
      901 ggctgatcgg tgtgccgact atatccagaa ggacttccct tgcctttgtt tctgcagaat
      961 cgtctttttt aaatccaaaa tcatttacaa ggtttgcact cgcccaggag accagatgtt
     1021 ttacaagtgt cttaggaatg gaactgttca ctgcatacat ggacatatga tccccgttgt
     1081 aagtagacca gccgagagcc atctctgaaa gatcacccgt accaagcaca aagcctccga
     1141 ccttgttcgc atagtccatc agtatctggg ttctctctct tgcctgacag ttttcaaacg
     1201 tgaccgatct gtcattcggg tcgagtccta tgtcctgaaa atgtttctgc actgcttcta
     1261 caatggatat ctcaagaaag gatgttccca tcacctttgc cagattttcc gaattgttct
     1321 ttgtgcggga acttgttccc attcccggca ttgaaatgca gaccactctg gagatatccc
     1381 atcccagatg agtcattgca cgtcttgcca caagaagagc gagagtcgag tcgagtcctc
     1441 cggagagtcc cagcacaata tgttttgact gtgtatgaac gagtcttgtc acaagaccgt
     1501 gagtgagaag atcaaagatt tcaatgcttc tttcggatag acttttttct cccttaggcg
     1561 caaaaggaaa aggatctaca gagcggagaa gtttttctga ttctgcattc agagctatgc
     1621 cggattcaga aagttcaatg acttctgttc tcggaacgga tctggcctgt tcctgaaatg
     1681 aacttgtctg tattctttca tgcctggtct ttttccaatc tatatccgca atcgcaatac
     1741 aatgtatgga agaattcatt ttttcaggat cagtctcgag aagtcgcgga cgtttttcat
     1801 caagcactgt cccgtattct gcaatgatgc agtgtcctga aaaaacgaga tctgtagtgg
     1861 attccgatgg acctgcagat gcataaacat aggaacctat gcatcttgcc gactgtcctg
     1921 atacaaggtc tttcctgtaa ccggatttgc ctgcaagttc gggagatgcg gacgggttga
     1981 atatgatgtc tgctccctgc aagtacagtc tggaggaggg agggtcggaa acccagagat
     2041 cctcgcagat ctcaattccg aagattactt caccttgccg gaaaagaaga gatccaaaag
     2101 ggatgttttt taatgatcta atattcacat ttctgaccgg tacggaagga gactgtcttg
     2161 taaaccatct gccctcgtaa aattctctgg agttcggcag aaaggctttc gggacaatac
     2221 cccgtatttc cctgtcctga aggactgctg cacagttcca gacccctcct tcgatttcca
     2281 gaggaacacc gacaattgca gtgatcttcg gaaggctgtc tttcagatga agcagacagt
     2341 cttctgcttt tttgagaagt gtcccttgag agaaaagatc tgcacaggta tatcctgtaa
     2401 tgcagagttc aggaaaaaga atgatttcac atccttttcc cgcagcatcc tcagcagctg
     2461 caagaatggc ggaagtgttt gtgtccggat ctccgaccct gaccttggga gatactgcag
     2521 cggctcttaa gaatccgtga gcacggggat tgtttaaaat gtcggggaga attttcatcg
     2581 aagagcctca gtagagcgca taatttatgt attcttttat gagaatcctg tgctgcaacc
     2641 ataaaaataa caaaattgaa tatcagatct tacagaagtt cgttcaggct gatttcaatt
     2701 tcagtatgcg gaattttgac tgagagattc ggttccagaa tggaatgaat attgtatttt
     2761 cct
//
LOCUS       DAOQGJ010000324         2752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22753_length_2752_cov_2.681127, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000324 DAOQGJ010000000
VERSION     DAOQGJ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22753_length_2752_cov_2.681127"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1544..2650
                     /locus_tag="PK683_06350"
     CDS             1544..2650
                     /locus_tag="PK683_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNH08105.1"
                     /translation="MSSIFQDISELGGMVMRNPKVAKDLFPSALSGFILNEGRNDTLQ
                     VQSHHKVFYDWKYKNDGNAEFLNLYKKAKKGQWDSDDLPWNTDVDPFNPEKPILPEKL
                     NPVYGTEVYNKTDKKTRGKLNHSLISLMLSQFLHGEQGALFAAGITVEATPWMNAKLY
                     GATQVMDEGRHVEVFQKYIVEKLDKLYKVNDNLYVIIHALTSTSDWDLKFLGMQIMIE
                     GLALGAFGLLYKFTNEPLLRELLKNVISDEARHVHYGVEALRDYYTKEVSESVRRDRE
                     DWAYEVAVLLRNRFLFLEFHQEHFAHTMSEEEWIKHVLASEIMSEFRRSLFSRLIPNL
                     KAIGLLSDRIRPKYEELGVLQYEGGNAADRSASL"
BASE COUNT          830 a          531 c          572 g          819 t
ORIGIN      
        1 aactgggaac gggaaaaaac ttctcaaatc gaacacccca gtctggcttt agaattattt
       61 ttatctgctc gcttaaaagg agtataatgc atagaatcag taacttttcc gaagcattag
      121 agttaaacgt ctgcaatgaa tcagtaaaga cagcggcttt cagatttgcg aaccctcaag
      181 cgaccgccag actttcctgc caaaaaccct catctgcagc cgaaggcggc cgaaggcagc
      241 cgaaggcgta agctgagatt ttaaaaggaa cttaaaaaaa atgaaaatgc tttatttcat
      301 actgatttgt attttgggct tatttcttgg ctgtactggt aaaaaggaaa acaaaaaccg
      361 aaatgtatat aaaaatttac tgacagtcgg tttggcatcc tttgctgcag actcggcttt
      421 cagtcagtat gcacagtcta atatcgatct aaaaaatttc ttaggaaaat ggtctgaata
      481 caaacgtata gataacagct tccagtcagg attatataat gtaactgctg aatatggaat
      541 cattgattca aatagaatct cagttagaaa ttctggaatt accaaatccg gttctgtttt
      601 cgatatagca gggattggaa ttatccctac cttaaaaaaa ccaggcatcg taaaagtttc
      661 atttttccct ttattttttt cagattacta cattttaaaa atcgatacag actgcagata
      721 cgctcttatt ggaagtcctg ttcctgaaat cctctggctg ctgtcacgca atggaagtta
      781 cccgccgtcc attgaatctg attatattca gtttgcaaac tctataggtt ataagacagc
      841 cctgcttcag tcttttactc aataagaatg aatcgattct ccttaaatag aacgtcattc
      901 tctgttaata atttttttca tacatgggtc caattatcaa gggtatgatt atacatttct
      961 gattcatcat ttttattctg cagctttctt ttcagtggtc ttttgaattc gtttttcttt
     1021 aatcaaaacg atcgtttttg tccgacttcc tttgtcgatt cccttcaggt cattcataaa
     1081 agtcttctca aatttactct agtcacataa agtatttgag ccagcattgc ttcgctgact
     1141 taaagaattc tgaaaaagat acccttatta tgtctcactt agagcttaat gatgtacgga
     1201 aatgaatctt ctgatctata ctttacacat tttcgatagt cctctcagtc gagaatgctt
     1261 agtttgtaag cagtttctca aaaaatctgc ttttggataa aaaaaataat tcttgcttga
     1321 ctttttagaa gcccttgtta attttatatc gatcattcgt tataaataaa acaaaggaga
     1381 acctcttctt tgaaccttgc ggcagttgcc ggtccgaatg atcagtgatc ggaatcaatg
     1441 gattgccgat ctttcaggat gttgggattt gcgatgcgta aaagcagtat gcacatcagc
     1501 aaagcagtat gcagaaaatg attaaaaaaa cgagtgaggc aatatgagca gcatatttca
     1561 ggacatttca gagttgggcg gtatggtaat gagaaaccca aaagtggcaa aggatctttt
     1621 tccttcagct ctttcaggtt ttattctcaa tgaaggcaga aacgacacac tgcaggtcca
     1681 gtctcatcat aaggtttttt atgattggaa atacaaaaat gacgggaatg ctgaattcct
     1741 gaatctttac aagaaggcca aaaaagggca gtgggattct gatgatctgc catggaacac
     1801 cgatgtggat ccgttcaatc ctgaaaagcc tatcctgccg gaaaaactga atccagtcta
     1861 tggtacagaa gtttataata agactgataa aaaaacgaga gggaagctga atcacagcct
     1921 gatttcactt atgctttctc agtttcttca cggagaacaa ggtgctcttt ttgcagcagg
     1981 aattactgta gaggctactc cctggatgaa tgctaaactg tacggtgcaa ctcaggtaat
     2041 ggatgaaggc cgtcacgtgg aagttttcca gaagtatatt gtggaaaaac tggacaagct
     2101 ttataaggtc aatgataatc tgtatgttat aatacatgca ctgacttcca caagcgactg
     2161 ggatctgaaa ttcctgggaa tgcagattat gattgagggg ctcgctctag gggcattcgg
     2221 tcttctctat aagtttacaa atgaacctct tcttcgcgaa cttctcaaga atgtaatttc
     2281 tgatgaagcg aggcatgtgc attatggcgt ggaagcactg agggactact atacaaaaga
     2341 agtcagcgaa tctgtccgtc gagacagaga agactgggct tatgaggtag cagttctgct
     2401 gagaaacaga ttcctctttc tggaattcca tcaggaacat tttgcgcata caatgagtga
     2461 agaagaatgg atcaagcatg tccttgcttc tgaaataatg agtgaattcc gcagatctct
     2521 tttcagcagg ctgattccga atctcaaggc gatcgggctt ctgtctgaca gaatcagacc
     2581 gaagtatgaa gagctgggtg ttcttcaata tgaaggtgga aacgctgctg acagatccgc
     2641 tagtttgtga agcatcgatg gtcggagcag cggttgtggc cgatgccatc ttacggcaag
     2701 cccacgggat gccgcgtcga cccgtctgta tcaccgaagc actcggaaac tt
//
LOCUS       DAOQGJ010000325         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22784_length_2749_cov_3.040831, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000325 DAOQGJ010000000
VERSION     DAOQGJ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22784_length_2749_cov_3.040831"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK683_06355"
     CDS             <1..492
                     /locus_tag="PK683_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNH08106.1"
                     /translation="EMGAGFLANSMGLLGDSLDMLADSIVYGLALYAVGGTELRKKKI
                     AGMSGYFQMFLALLGLGEVIRRFIEPEPLPGFLVMIVISVLALIGNALCLYLLQKSRS
                     SEAHMQASMIFTANDVIVNIGVIIAGGLVYLTDSKYPDLLVGIIVFLLVGQGALRILK
                     LSK"
     gene            603..2129
                     /locus_tag="PK683_06360"
     CDS             603..2129
                     /locus_tag="PK683_06360"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH08107.1"
                     /translation="MQPLPIGIQTFRDLREGNFLYVDKTKQIYPLIEHSKGAYFLSRP
                     RRFGKSLTLSVLDEIFSGNRELFKGLWIYDSVWKWKTHPVLRFDFSKQKAKNKEELVK
                     FLFYQLEDVSKRYGISLERSTYYDQFQELIAKLGQKEKVVILIDEYDKPIIDHLENTE
                     LAVEMREVMKGFFTVLKGSDPYIRFLLLTGVSKFSKAGVFSNLNHLEDITMEDSFSDL
                     VGITEDELVNNFSDYISEFAKEGKLNNNELTDRIREWYNGYRFSPKGISVYNPFSTLL
                     LLKHKRFEHHWFETGTPEFLIKLIIKNNYNVMEIPFKAEAVHFSSYEVDRLSITPLLL
                     QTGYLTIKEYDPAAGVYTLDYPNYEVKQAFLNYLFGFMKREETGSIIFKLYTAFSEDS
                     LEDCIDILREIFLSINYDIQIRQEKYWQTVFYLIFTLLGFRIQAEVKTNKGRIDAVVE
                     SKSIYIFEFKLNGTKEEALAQIKDKKYYEKYLSIGKPVILVGAEFKDRNIGDYLTEAV
                     "
     gene            2247..2606
                     /locus_tag="PK683_06365"
     CDS             2247..2606
                     /locus_tag="PK683_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HNH08108.1"
                     /translation="MKKRVLMGILLAAALVSVSLYAAGDMFGSLAFNKDTDAYGWAVD
                     FETQGEAAALKSCGDGCDVVIQFQNTCAAFALGDNHSWGWVHSVNKSVAQKNAVAKCS
                     KYGTGCTMKVWGCTTRQ"
BASE COUNT          820 a          529 c          599 g          801 t
ORIGIN      
        1 gaaatgggtg caggattttt ggcaaattca atgggtcttt tgggggacag tctggatatg
       61 ctcgctgaca gcattgtgta tgggcttgct ctttatgcag tcggagggac tgaactcaga
      121 aaaaagaaaa ttgccggtat gtccggatac tttcagatgt tccttgctct gcttggactg
      181 ggagaagtga tacgccgttt cattgaaccg gaacctcttc cgggatttct ggtgatgatc
      241 gtgatttctg tcctggctct catcggaaat gctctctgcc tgtatcttct gcagaaaagc
      301 agaagcagtg aagcgcatat gcaggcaagt atgatattca ctgccaatga tgtcattgtc
      361 aacataggcg taattatcgc cggaggactt gtctatctga ccgattccaa gtaccctgac
      421 ctgcttgttg gaatcatcgt attccttctc gtcggacagg gagcactcag aatactgaag
      481 ctttcgaaat aaattttccc tgtttccagt caatgaaccg aaaatattct gcagtgaatg
      541 ccaatttgaa tacatttgga ttctcattga ctttcatatg tttccggcaa agatagacct
      601 gtatgcagcc cctacctatt ggaatccaga ctttccgcga tttacgggaa ggaaattttc
      661 tttatgttga taaaacaaaa cagatttatc ctctgattga acactcgaaa ggcgcctatt
      721 tcctgtcccg tccgcgccga ttcgggaaaa gtctcactct ttctgttctc gatgaaattt
      781 tttcgggaaa cagagaactt tttaaaggac tgtggattta cgattctgtc tggaaatgga
      841 aaacacatcc agtgcttcgg tttgatttca gtaagcaaaa ggcaaaaaac aaggaagaac
      901 tagtcaagtt tttattttat caattggaag atgtttcgaa aaggtatggt atttcactgg
      961 aacgatctac ttattatgac cagtttcagg aacttatcgc aaaactggga cagaaggaga
     1021 aggtcgtaat cctgattgat gagtacgaca aacccattat cgatcattta gagaatacgg
     1081 aactggcagt cgaaatgcgg gaagtaatga aaggattttt tacggttctg aaaggctctg
     1141 atccgtacat aaggtttctg ctgctgacag gagtaagcaa gttttccaag gctggagtat
     1201 tcagcaacct gaatcatctg gaagatatta ctatggaaga cagtttttct gatttggtcg
     1261 gaattaccga agatgaactt gtaaataatt tctctgacta tatttcggag tttgcaaaag
     1321 aaggaaaact gaataataat gaacttactg acagaatccg ggaatggtac aacgggtaca
     1381 ggttttctcc caaaggcatt tccgtgtaca accctttttc cactcttctt cttcttaagc
     1441 ataaacggtt cgaacaccac tggtttgaaa caggaacacc tgagttttta attaaactga
     1501 taattaaaaa caattataat gtaatggaaa taccttttaa agcagaagcc gtccattttt
     1561 ccagctatga agttgaccgt ctgagcatta caccgctcct tctgcagacc ggttatctga
     1621 ctatcaagga atatgaccct gccgctggcg tatatactct ggattatccg aactatgaag
     1681 taaaacaggc tttcttaaat tatctgttcg gttttatgaa aagggaagaa accggttcca
     1741 taatattcaa actgtacact gctttttcgg aagacagcct ggaggattgc atcgacatcc
     1801 tgagagagat tttcctgagc atcaattacg acatacagat cagacaggaa aaatactggc
     1861 agacagtatt ctatctgata ttcactctgc ttggcttccg cattcaggct gaagtgaaaa
     1921 ccaataaagg ccgaattgat gccgttgtag aatcaaaatc catttatatt tttgaattca
     1981 aactgaacgg aacaaaggaa gaagcacttg ctcagatcaa agataaaaaa tattatgaaa
     2041 aatatctttc cattgggaaa cctgttattc tagtcggtgc tgaatttaag gacagaaaca
     2101 taggagatta tctgactgag gcagtttagt ttcatactcc tgcctgtact caggaatact
     2161 gccggtcact gacagccggt gaaattattt tctaatcgct tgacgaaaaa tgagtaatgt
     2221 gaatgatcgt gtcccacgag gtgattatga aaaaacgagt attgatgggt attctgcttg
     2281 cagcggcatt ggtgagtgta tctttatatg cagccggaga tatgttcgga tctcttgcat
     2341 tcaataagga tactgatgct tacggctggg cagtcgattt tgaaacacag ggcgaagcgg
     2401 ctgcattaaa atcctgcggt gacggatgtg atgtagtcat acagttccaa aacacttgtg
     2461 ctgcgtttgc acttggagac aatcactcct ggggttgggt gcacagtgta aataaatcag
     2521 tagctcaaaa aaatgctgtg gccaaatgct ccaaatatgg aacaggatgc acaatgaaag
     2581 tttggggctg cactaccaga cagtaaaatc aaaaaataaa gatctccagt tatctttaat
     2641 atctccagca gatgcgaata cttccaaatg atgtattgcg catatgattc attttcctcc
     2701 tctatatcag attcacaatg acaccggcag attttgagta tccgatttt
//
LOCUS       DAOQGJ010000326         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22820_length_2746_cov_3.815682, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000326 DAOQGJ010000000
VERSION     DAOQGJ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22820_length_2746_cov_3.815682"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK683_06370"
     CDS             <1..382
                     /locus_tag="PK683_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNH08109.1"
                     /translation="SSCTGINIVSSSLGIGEVTNPSPDYAHPLKFIKGGGVYHRNYVP
                     LGTGRNSENSIKAQGCSRSVLFLAAWGDSSYSGIMKKSGIRKIAYSEAEQFAVLGSVY
                     HSFCNILYGTDSADPARKEEKKGK"
     gene            384..689
                     /locus_tag="PK683_06375"
     CDS             384..689
                     /locus_tag="PK683_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HNH08110.1"
                     /translation="MIKYFIIIAVIFSRCAIGPVNGALFTDTNFPGQFNSENNVAPDR
                     SASGCQHSILGLFSWGDAGAGSTAHRNGITKIAVIDHSAFSVLFSAYRNYCTKVTGE"
     gene            692..1021
                     /locus_tag="PK683_06380"
     CDS             692..1021
                     /locus_tag="PK683_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNH08111.1"
                     /translation="MKKYFITIVLSILFFSSCSSPAGGIFFTNTVQNVHSDGLQGQIS
                     SASVQKSGKSCSLSGLPVSLFYYGAGNSIDEAKKNGDISKIALIDRESMTVFFFFYRE
                     CIVVWGE"
     gene            1024..2061
                     /locus_tag="PK683_06385"
     CDS             1024..2061
                     /locus_tag="PK683_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08112.1"
                     /translation="MMSAISKICFLSAVIFSFQTSAKDTVHEKPTDFTDPDNHRFIIR
                     AGSSVLGGVRPPEADENNKSAQVYNVMDRIFFPNFYQYGMAYVQKNSDILNSRLEYRY
                     KDKYRIFRDTLAIRDSSTYLNYSITMTSSSGNILQPLGGPLQPWKENRESIGIAYYHP
                     ISKTFSFGASLKSANLSQSYNLSFATIFGIYTSNNVVFGNAEKGNIQTSGYIPGIGME
                     WKPLRWFEIHYTLEQYILSGKKDSVQSIFVVGRSVNVSVYDLRSGNFTYTGTSQNLDF
                     VFRFSSWFAARWGIVSERFKRKYDSFISSDDYSTSLAYGAASSFLYPKIVSASFHNDY
                     LRFQVEFSKGF"
     gene            complement(2062..>2746)
                     /locus_tag="PK683_06390"
     CDS             complement(2062..>2746)
                     /locus_tag="PK683_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH08113.1"
                     /translation="AKALKVNLGDELTFNVQGTEISGKFTNTRIVNWADMKPNVVVLF
                     SPGVLEGAPSYVISSMYIPDNDKRYQFQKELIRLCPNVSVYDAEKTLSRVNILIERVA
                     GIAELMTGLILACSLLLLTSALFLQLKEKIVSFALLKVSGAGDGFLKKVILTEAALFS
                     FFSFFTALVLSSVSCWIFTEYFLKVNFSFPFFRILIFGTVYLFTIIVFYILSMRKAVL
                     SNPKSVFRM"
BASE COUNT          831 a          585 c          555 g          775 t
ORIGIN      
        1 atcatcctgt acgggcatca atattgtttc aagttcactt ggaatcgggg aagtgacaaa
       61 cccctctccg gattatgctc accctttgaa atttatcaaa ggcggaggcg tctatcaccg
      121 aaactatgta cctctcggaa caggaaggaa ttcggagaat tctataaaag cacaggggtg
      181 cagccgttct gtgctgtttc tggctgcctg gggtgattcc tcttattcag gaattatgaa
      241 aaaaagcgga atcagaaaga ttgcctattc cgaggcagag cagtttgcgg tcttaggttc
      301 tgtctatcac agtttctgca atatcttata cggcacagat tcagctgatc cggccagaaa
      361 ggaagaaaag aaaggaaaat aatatgatta aatattttat aattatagca gtcattttca
      421 gcagatgtgc aatcggccct gtaaacggtg ctctttttac agatacaaat ttccccggtc
      481 agttcaattc agaaaacaat gtcgcaccgg acaggagtgc ctccggctgt cagcattcaa
      541 ttctgggact tttcagctgg ggtgatgccg gtgccggatc gaccgcgcac agaaacggaa
      601 tcacaaaaat cgcagtcatt gatcattccg ctttcagtgt tcttttttct gcctacagaa
      661 actactgtac aaaagtaaca ggagaataat tatgaaaaaa tattttataa ctatagtttt
      721 atcgattctt ttcttttcct cctgctcttc tcctgccgga gggattttct ttacaaacac
      781 agtgcagaac gtacactcag acggcctgca gggacagatt tcatcagcct ctgtccagaa
      841 atccggaaaa tcctgctctc tttccggtct gccagtcagc ctgttctatt acggagcagg
      901 aaattccatt gacgaggcaa aaaagaacgg agatatttcc aaaattgcat tgatcgacag
      961 agaatctatg actgtgtttt tcttctttta cagagaatgc attgtagtct ggggagaata
     1021 aaaatgatgt ctgctatatc aaaaatttgt tttctttctg ctgtaatttt ctcctttcag
     1081 acttccgcga aagatacggt ccacgaaaaa ccgaccgatt ttacagaccc ggacaatcac
     1141 cgattcatca tccgtgcggg aagcagcgtt ctcggaggag tgcgtccacc tgaagcagat
     1201 gagaataata aatcggctca ggtatacaat gtcatggaca gaattttttt tcctaacttc
     1261 tatcaatacg gaatggccta tgtacagaaa aactcagata tattgaattc ccgcctggaa
     1321 tacagatata aggacaagta cagaatattc agggatacac tagctataag agacagcagc
     1381 acatatctga attattcaat tacaatgacc agctcttcgg gcaatatact tcagccgcta
     1441 ggaggtcctc tgcagccctg gaaagaaaac agagaaagca taggtattgc atattatcat
     1501 ccaatttcaa aaaccttttc ctttggagct tctctgaaaa gcgcaaactt aagtcaatcc
     1561 tataatcttt cctttgctac gattttcggt atctatacaa gcaacaatgt cgtttttgga
     1621 aatgcagaaa agggaaacat tcagacatca ggatatatcc ccggtatcgg tatggaatgg
     1681 aaaccgctcc gatggtttga aattcattat acactggaac agtacattct ttcaggaaaa
     1741 aaggattcag ttcagagcat tttcgttgta ggacggtcag tcaatgtttc cgtgtacgat
     1801 ctgagaagcg gcaatttcac ttataccgga acatctcaga atctggactt tgtattccgt
     1861 ttttccagct ggtttgctgc aaggtgggga atcgtctcgg aaagattcaa aagaaaatat
     1921 gattcattta tttcctcaga cgactacagt acaagtcttg catacggtgc agcaagttca
     1981 tttctctatc ccaagattgt atctgcatca tttcataatg attatctccg ttttcaggtc
     2041 gaattcagca agggattctg attacatccg aaatacggat ttaggattcg aaagcacagc
     2101 ctttctcatg ctcagtatat aaaatactat aatagtaaat aaatatactg ttccgaaaat
     2161 gagtattctg aaaaacggaa atgaaaaatt gactttcaga aaatactctg taaatatcca
     2221 gcaggaaaca gaggacagaa caagtgcagt aaaaaaactg aaaaaggaaa acagggcagc
     2281 ttctgtcagg atgacttttt tcagaaatcc atcccctgcc ccgctgactt tcagaagtgc
     2341 aaacgaaaca attttttcct tcagctgaag aaaaagtgcg gaggtcagaa gaagcagact
     2401 gcaggcaaga atcagtccgg tcatcagttc tgcgattcct gccactcttt caatcagtat
     2461 gttcactctg ctcagtgttt tttcggcatc atagacagat acattcggac acagcctgat
     2521 aagttccttc tgaaactgat atcttttatc attatccggt atatacatag agctgataac
     2581 ataggatgga gctccctcca gaactccggg ggaaaaaagc acaaccacat tcggtttcat
     2641 gtcagcccag ttcacaattc tggtattcgt aaattttcct gatatctcgg tgccctgcac
     2701 attgaatgtg agctcatctc ccagattcac tttcagggcc tttgca
//
LOCUS       DAOQGJ010000327         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22824_length_2746_cov_3.634337, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000327 DAOQGJ010000000
VERSION     DAOQGJ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22824_length_2746_cov_3.634337"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK683_06395"
     CDS             complement(<1..546)
                     /locus_tag="PK683_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNH08114.1"
                     /translation="MTREKYLETSNSFHRSVGLGKAILHMIGNMIGVGIFIYPAIIAS
                     HLPHPAWFFFVWIIGGLIALTGALSSAELGTYYPEAGGDYAYLRHGYGRRWAFVYGFL
                     TFFITFPGSIALGVGLAVHYQGASLLGPWVRDVFFQSELLDFRLYNFQIISVGIIFIL
                     TLLNHRGLKTSFFMQKIMTLLP"
     gene            742..1518
                     /locus_tag="PK683_06400"
                     /pseudo
     CDS             742..1518
                     /locus_tag="PK683_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            1475..1882
                     /locus_tag="PK683_06405"
     CDS             1475..1882
                     /locus_tag="PK683_06405"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HNH08115.1"
                     /translation="MDSDALKILRKSYSEALYRINTSAGQITLRTEENWDIHEKIFPC
                     NEFIYITAWNPGSEPLPEPENRKNNFLLLSELKKTASFILEGCSESESGEWKEESFFA
                     ADVSLETGKILQKQFGQNAFLYGRKGEKIKLIF"
     gene            complement(2020..2433)
                     /locus_tag="PK683_06410"
     CDS             complement(2020..2433)
                     /locus_tag="PK683_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNH08116.1"
                     /translation="MKEKIFVDTNILIYAHDKKSPQKREIASRLLESLWDMEEKPYIS
                     VQVLQEFFVNISRFLTYDEVSELMEIYKNWNVIDNSLSVFEKAVLIYRKHKLSFWDSL
                     IVSAAVMSGSKILFTEDLSHGQKIESVKIINPFME"
     gene            complement(2411..2668)
                     /locus_tag="PK683_06415"
     CDS             complement(2411..2668)
                     /locus_tag="PK683_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08117.1"
                     /translation="MDKNVTLRIDENILKMCRFKAVEENKSLSKWLSDLMEREINESQ
                     INESREYQRVREKAKKILSKGYNLGGRPLKREDIYERKNIR"
BASE COUNT          866 a          585 c          567 g          728 t
ORIGIN      
        1 cggaagcaga gtcataattt tctgcataaa aaaggaggtt ttcagtcctc tgtgattcag
       61 cagagtgaga ataaatataa tacctacaga gataatctga aaattataca gacgaaagtc
      121 aagcagttcg gactgaaaga agacatccct gacccaggga ccgaggagcg aagctccctg
      181 ataatggaca gccagaccga cgccaagagc aattgacccc ggaaatgtaa tgaagaaggt
      241 gagaaatccg tatacgaatg cccatcgtct tccatatccg tgccgaagat aggcatagtc
      301 cccgcccgct tcaggataat aagtcccaag ttcagcagaa ctcaatgcac ctgtgagcgc
      361 aatcagtccc ccgatgatcc agacaaaaaa gaaccatgca ggatgaggaa gatgagacgc
      421 tatgattgca gggtaaatga aaatccctac cccgatcata tttccgatca tatgtaaaat
      481 tgcctttcca aggccgacgc tgcgatgaaa tgaattcgaa gtttccaagt atttttccct
      541 tgtcactgtt tttcagtttc tcaggatgtc aaatagttac tgccaatatt gagcaagtca
      601 aaaacgaccg gagacctttc attgaaaaaa gttctgttat tctgcattat gattttaatt
      661 cccactctct tctgcataag agaagtcaag aatatttccg ctgaagaaaa aaaagcacct
      721 gcctgtctga acagtttccc tggaatgtcc tgcattccgg caggagagtt tattcgggga
      781 agcaataagc acggtgcaga tgaaagaccc gaagcaaaag tttatgtttc tgaattttat
      841 atggacatca acgaagtcac gaacgaagaa ttcaacaaat gccttgccga aggaaaatgc
      901 aaggagtgcc tgaaaaacaa aacctgcact tatgtcggtg caaaatatgg aaaaagatac
      961 cagggtccca agcagcccgt atccggaatc agctggttca ctgcaaaaga atactgtgaa
     1021 tgggctggta aacgtctgcc tacagaagca gaatgggaaa aagcggcgag gggaactgac
     1081 ggagatattt atccctgggg aaatgaagaa gctgactgta caagagccat cattatggac
     1141 agacagggaa gaaaaggctg tgctcctaaa aagctgagcc cggactggca tatgcctact
     1201 cagaacgtcg gatccagacc cgcaggcaaa tacgggcttt acgatatggc aggaaactcc
     1261 tgggaatggg taagcgactg gtacgcacca tatgccaaat gcggcgattc ctgtcaggga
     1321 agagacccga aaggtccctg cggaggtgca gacaaatgtc caggatacac tcacaaggtt
     1381 ctgaaaagcg gttcctggtg gtggtcggcc tattatgcca gatcatccaa cagaaggcat
     1441 aatgatccgg gattcaagga gcagtaccat cattttggat tcagatgcgc taaagatcct
     1501 cagaaaatcg tacagtgagg ctctgtaccg aataaatact tccgcaggac agatcaccct
     1561 gcggacggaa gagaactggg atattcatga aaaaatattc ccgtgtaatg aatttatcta
     1621 tattaccgca tggaacccgg gttcagagcc tttgcccgaa ccggaaaaca gaaaaaacaa
     1681 tttccttctt ctttctgaac tgaaaaaaac agcatcattc attcttgaag gctgcagtga
     1741 atccgaatca ggagaatgga aagaggaatc cttttttgca gctgatgtca gtctggaaac
     1801 aggaaaaata cttcagaaac agttcggaca gaatgctttt ctgtatggac ggaaaggaga
     1861 gaaaataaaa ctcattttct aaccgctttt tatttcggag tacccctcat tgtctttacg
     1921 ttggcactgg gtttaatagg agcagctctt tattatacag gaaggtttat attcaagttc
     1981 tgatagttag agtgccggaa aatctgttat tttgcagacc tactccataa aaggatttat
     2041 aatctttaca ctttctattt tctgtccgtg actgagatct tcagtaaaca gtatttttga
     2101 tccgctcatt acagctgcac ttacaatcaa actgtcccag aaggacagtt tatgcttacg
     2161 gtaaatgaga accgcctttt caaaaacaga aagagaattg tctatgacat tccagttttt
     2221 atagatttcc atcagttcag aaacttcgtc atacgtcaaa aaccgactga tatttacaaa
     2281 gaactcctgc agaacctgaa cactgatata aggcttttct tccatatccc aaagtgattc
     2341 caaaagcctg gatgctattt cccttttttg cggagacttt ttatcatgtg cataaatcag
     2401 tatatttgta tcaacgaata ttttttcttt cataaatgtc ttctctcttc aaaggtctgc
     2461 ctccaagatt ataacctttt gaaagaattt tctttgcttt ctcccttact ctctgatatt
     2521 ctctggattc atttatctga gattcattta tctctctttc cataagatcc gaaagccatt
     2581 ttgaaagaga tttgttctct tcaacggcct tgaatctgca catctttaaa atattctcat
     2641 cgattctgag cgttacattt ttatccattg ccgcctccat ttacacagaa acacatttac
     2701 acagaaacac aagaataatt tgagtaaaat tccagtaaac gccttt
//
LOCUS       DAOQGJ010000328         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22863_length_2744_cov_3.323912, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000328 DAOQGJ010000000
VERSION     DAOQGJ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22863_length_2744_cov_3.323912"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2397)
                     /locus_tag="PK683_06420"
     CDS             complement(<1..2397)
                     /locus_tag="PK683_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH08118.1"
                     /translation="MNLSLISIRNPVFAWMLMTALMFFGAISFKRLGISQMPDVDFPV
                     INVSLSLPGASPEIMESTVTDPVEDALLSVEGITGLNSTSGFGTANITVDLELNRNVD
                     SALQEIQTKVLRVQKLLPKDLDPPVITKYNPEESPIMWVGASYTGERKVLMKYVQDHL
                     KDKFSTVPGVGQIMLGGHLEPALRVWVTPKALNSRELTVEDIIQTIQREHIEVPAGML
                     ETDKKVMSIRAMGEAETIDIFGKLPIAYRGGTPIFDGGYRLSTTAELEDGLEDVYRLS
                     RFNGKSAVGIGIMKQRGVNAVETAEAVKKRMEELRKTLPEGFELGINFDTTRFIKESM
                     DELEFNLILSAVLTSIVTFLFLGSLKSTLNILLAIPTSILGTFIFLDWIGFTLNTFTL
                     LALSLAVGIVVDDAIMVLENIYRFREQGETFYNAAKKGARQISFAAAAATFSIVAIFL
                     PVAFMKGIIGKYFYQFGVTITAAVMLSLLESLTLTPMRLSRFSGSDIRIERLSGWIER
                     GFLATERLYERVTAFCIKWKYSVIIISTVLAASSFLILKPLKKEFVPSQDQSQFIVRI
                     QTVPGSSLAYTDGFAAETEKILLKNKDVDRVYSIIGAGRGQTGSNSAMFFVSLKQPEE
                     REINPKTKKRYTQSEIAGEIRKSVSGISKELKVFIQDLSMRGLSGSGRDYPVEFVIQG
                     SDWDTLAALSGKIMSEMENTKKYTDIDTNYKPGQPEIHIIPDRAKALAYGVSMQSISN
                     TIGAMIGGIRAGQFSKDGRRYNIYVRVKDEFRKQESDIKNLFVRNSRGELVRLADLVK
                     T"
     gene            complement(2400..>2744)
                     /locus_tag="PK683_06425"
     CDS             complement(2400..>2744)
                     /locus_tag="PK683_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08119.1"
                     /translation="RQAESLKRQAVLRFIQTEKFAVQEVKKAFHHYKNSLLQEKNYSG
                     ILTNASRTYSAVIADYDLKQATLLEVLDSLSTLQTAKDELINSKFQKQLDLISLDIAI
                     GKEPVPDSYKEN"
BASE COUNT          717 a          655 c          561 g          811 t
ORIGIN      
        1 agtctttacc agatctgcca gtcggacaag ttccccccgg ctgtttctga cgaaaagatt
       61 tttgatgtcg gattcctgct tcctgaactc atccttgacc cgtacataaa tattgtacct
      121 ccttccatcc ttggaaaact gtcctgcccg gatgcctccg atcattgcac cgattgtatt
      181 ggatatgctc tgcatgctta caccataggc cagagccttt gcccggtcag gtatgatatg
      241 aatttccggc tgacctggtt tataattagt atctatgtca gtatattttt ttgtattctc
      301 catctctgac atgatctttc ctgaaagcgc agcaagagtg tcccagtctg atccctgtat
      361 cacaaattcc acaggatagt cccttcctga acctgaaagc cccctcatcg agagatcctg
      421 aataaatact ttcagctcct ttgaaatccc tgaaacggat ttcctgattt ctccggcaat
      481 ttcagactgg gtgtatctct tcttagtctt gggattgatt tctctttctt ccggctgctt
      541 cagactcaca aaaaacattg cggaattgga acctgtctga cctcttcccg cacctatgat
      601 ggaatatact ctgtccacat ccttgttttt cagaagaatt ttttccgttt cagcagcaaa
      661 accgtcagtg tatgcaaggg atgagcccgg aacagtctgg atccttacaa tgaactgact
      721 ctgatcctgc gaagggacaa attctttttt cagaggctta agaatcagaa atgatgatgc
      781 agccaggaca gtggaaatta taattacact gtatttccat ttaatgcaga atgcagtcac
      841 ccgctcatac agcctttccg tggcaagaaa gcctctctcg atccacccgc tgagtctttc
      901 tatgcggata tcggatcctg aaaaacggga gagcctcatc ggagtgaggg tgagagattc
      961 aagaagcgat agcattactg ctgcagtgat agtcacccca aactgataaa aatactttcc
     1021 gatgatgcct ttcataaatg ctaccggcag aaaaattgcg acaatggaaa atgttgcagc
     1081 cgcagctgca aaggaaatct gcctggctcc cttttttgca gcattataaa atgtttcacc
     1141 ctgttccctg aacctgtaaa tgttttccag aaccatgatt gcatcatcca ctacaatccc
     1201 cactgcaaga gaaagtgcaa gaagagtgaa tgtattcaga gtgaatccga tccagtccag
     1261 aaatatgaat gttccgagaa tcgaggtagg tatggcaagc aggatgttca gtgtggattt
     1321 taatgatccc aaaaaaagaa atgtgacaat gcttgtgagc accgctgaaa gaatcaggtt
     1381 gaattcaagt tcatccatgg attctttgat gaatcttgtc gtatcaaaat tgattccaag
     1441 ttcaaaccct tccggcaggg tcttcctgag ttcttccatt cttttcttca cagcctctgc
     1501 agtctccact gcattgactc ctctctgctt cattattcct attccgacgg ccgactttcc
     1561 gttgaatctc gaaagtctgt acacatcttc cagtccgtct tccagctctg cagttgtgct
     1621 cagtctgtaa cctccgtcaa atataggtgt accgcctctg taggctatag ggagctttcc
     1681 gaatatgtca atggtttctg cctcccccat tgcacgtatg ctcatcactt ttttatctgt
     1741 ctcaagcata cctgccggaa cttcaatatg ttctctctga atcgtctgaa tgatgtcttc
     1801 tacagtgagc tccctggaat tcagtgcttt aggagtaacc cagacacgca gggcaggttc
     1861 aagatgtcct ccaagcataa tctgaccgac accgggaaca gtggaaaatt tatccttgag
     1921 atgatcctgc acatatttca tcagaacctt tctttcaccg gtgtacgatg cacctaccca
     1981 cataatcgga gattcttcag ggttgtattt tgtaatgacc ggcggatcca gatccttagg
     2041 aagaagtttc tgaacgcgaa gaacttttgt ctgaatttcc tgaagggcag agtccacatt
     2101 ccggttcagt tcaagatcca cagtaatatt cgctgttccg aatccggagg tagaattcag
     2161 accggtaatt ccttccacgc ttaaaagggc atcctccaca gggtcggtca cggtcgattc
     2221 cataatttca ggagatgcgc cgggaagact gagacttaca ttgatgactg gaaaatccac
     2281 atcgggcatc tgagatatcc cgagtctctt gaatgaaatc gctccgaaaa acatcagagc
     2341 tgtcataagc atccaggcaa atacaggatt tctgatggaa attaatgata aattcattat
     2401 taattctcct tataggaatc aggaaccggt tcctttccga tggctatgtc cagagatatc
     2461 agatccagct gcttctgaaa cttggaattg atcagttcat cctttgctgt ctgaagtgta
     2521 ctgagcgagt caagtacttc aagaagagtt gcctgcttca gatcatagtc tgcaatgact
     2581 gcactgtatg ttctggaggc atttgtgagg attcctgaat agtttttttc ctgaagaagt
     2641 gaatttttat aatgatgaaa tgccttcttt acttcctgta cagcaaactt ctctgtctga
     2701 atgaaacgga gaactgcctg tcttttcaat gactctgcct gtct
//
LOCUS       DAOQGJ010000329         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22901_length_2741_cov_3.760983, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000329 DAOQGJ010000000
VERSION     DAOQGJ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22901_length_2741_cov_3.760983"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK683_06430"
     CDS             complement(<1..991)
                     /locus_tag="PK683_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH08120.1"
                     /translation="MTAVNGVFDLSQWNFEGQERDSTVQLDGEWEFYWNSFLFPNGLS
                     DAASYIKVPSQWQDEKILLDKKEFTPPGKGYATYRLKIILPQKTPPLSIIIPTAGSAY
                     RVYINRELYFSFGNPGTKKEESVPFSRKKDFAVSLPNDRVLDLVIHISNFHDTSGGLW
                     DNFILGTSKAVSEKQKYVFAVDLIVFGSLLVMGLYHLGLYLNRRKDSSPLYFGIFCLL
                     IAVRTVTVNERLILDFIPKMSFLLMHKVEFFSFYFSAFIFLLFIQRLFPEESSKKMFM
                     IMTFIFIPPSLSVIIFPMNIYTEFLGIVRISILIGIIYVMKISVQSLMGKKQGA"
     gene            1222..2070
                     /gene="dapF"
                     /locus_tag="PK683_06435"
     CDS             1222..2070
                     /gene="dapF"
                     /locus_tag="PK683_06435"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNH08121.1"
                     /translation="MNLIRFTKMEGIGNDYVYIDATKDNIKLTPEQIIRISDRNFGVG
                     SDGVIFIRSSDKADFMMDMYNSDGTSSEMCGNGVRCVGKFIYDKKLTSKKNPKVETGK
                     GVLELDLETDSSDRVTYVTVDMGEPILVPEKVPVKISGTDPVIEKELTIEGSDLKFTA
                     VSMGNPHAVIFVDDNKKFPVREIGPLIENNPLFPRRTNVEFITVLGKDHLHQRTWERG
                     AGETLACGTGACATAVAAHLTGRAGRKVRIDLLGGTLYIEWRSSDSHVIMKGPAVTVF
                     EGEISV"
     gene            2070..2723
                     /locus_tag="PK683_06440"
     CDS             2070..2723
                     /locus_tag="PK683_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNH08122.1"
                     /translation="MNTSVFPYIGIITLISVIKLFYALGTELIPDEAYYWEWSRRLDL
                     SFYDQGPGVGLYIRLFTSILGNTNFALKLAAVSAGFGTSVFLIYTSRLLQFGKKGLFW
                     TVLLSQLIPGFFGGSIFIMHDSPLLLSWSAALYFTVHYIFKKDVLSLYLMFLSLGFGA
                     LSKHSMVFFALSLIVWLILNPKQSALLKNPHFYAGLILAGAVFSPVILWNIQHNRIS"
BASE COUNT          858 a          539 c          569 g          775 t
ORIGIN      
        1 ctgcaccctg tttcttcccc atcagactct gcacggaaat cttcatcacg taaataattc
       61 ctattaatat tgaaatcctt acaataccca gaaattctgt atatatattc ataggaaaaa
      121 taattacaga taaggaagga ggaataaaaa taaaagtcat tatcataaac attttctttg
      181 aactttcttc aggaaaaagc ctttgtatga aaagcagaaa tatgaaggca gagaaataga
      241 atgagaaaaa ttcaacctta tgcataagca gaaaggacat tttcggaata aaatcaagga
      301 ttaacctttc gtttacagtg accgttcgga ccgcaatgag aagacagaaa attccgaaat
      361 aaaggggaga gctgtctttt cttcggttga gataaagccc caaatgataa agacccataa
      421 ccagaagaga gccgaaaacg atcagatcga cagcaaagac atatttctgt ttttctgaaa
      481 cggctttcga tgttcccaga atgaaattat cccaaagacc tcccgaagta tcatgaaaat
      541 tggatatatg aatcacaaga tccagaactc tgtcatttgg aagactgact gcaaaatctt
      601 tttttctgga gaaaggaaca gattcttctt ttttggtgcc ggggttcccg aaggagaaat
      661 agagttctct gttaatatat accctgtatg cagatccggc agtaggaatg atgatcgaaa
      721 gaggcggtgt tttttgagga agaatgattt tcagcctgta tgttgcgtaa ccctttccgg
      781 gaggagtaaa ctctttttta tccaaaagta ttttttcatc ctgccattga gaaggaacct
      841 taatatacga tgctgcatca gaaagaccat tcgggaagag aaaactgttc cagtagaatt
      901 cccattcacc gtccagctga acagtgctgt ccctttcctg accttcaaaa ttccactgac
      961 tcaggtcaaa tactccattg acagcagtca cccgcgttct ttcatcagtg cagttaaaaa
     1021 gaaaaacagc cataaaagaa aagaaaaata atctgaaaag ctgttgaata aacctcacag
     1081 tgataatatc aaataatctg cccatttctg taacgcactt ttcatttcca ttaactcagt
     1141 cttttatttt ctgaaaactg cttgtcagca gaattgaggt tcagaaagtt taatcaagag
     1201 tctatattaa acaggcggca tatgaatctg attcgtttta caaagatgga aggcattgga
     1261 aatgattatg tatatattga tgcaacaaag gataatataa aacttacccc ggaacagatc
     1321 atcagaatct cagacagaaa tttcggagtc ggaagcgacg gagtcatatt catccgcagt
     1381 tctgacaagg ccgattttat gatggatatg tacaattcag acggaacgtc ttcggaaatg
     1441 tgcggaaacg gagtaagatg cgtaggtaaa tttatttatg acaaaaaact gacttccaaa
     1501 aaaaatccga aagtggaaac cggcaaagga gtgcttgagc tcgatcttga aactgacagt
     1561 tcagaccgag tgacttatgt gactgtcgat atgggagagc cgattcttgt gccggaaaaa
     1621 gttccggtaa agatttcagg aaccgatcct gtcatcgaaa aagaacttac aattgaagga
     1681 tctgatctga aatttactgc ggtaagtatg ggcaatcctc atgcagtcat ttttgtggat
     1741 gacaataaaa aatttccggt ccgcgaaata ggccccctca ttgaaaacaa tcctctgttt
     1801 cccagaagaa caaatgtgga attcatcaca gtattaggaa aggatcatct gcatcagaga
     1861 acctgggaaa ggggtgcagg agaaaccctg gcctgcggaa caggggcatg cgcgactgca
     1921 gtagctgctc atctgactgg aagagcaggc cgaaaagtaa gaattgatct tctgggcgga
     1981 acactttaca tagaatggag atcatctgac agtcatgtga ttatgaaagg acctgcagtc
     2041 acagtatttg aaggagaaat atccgtctga tgaatacttc agtatttcct tatatcggaa
     2101 tcataacact catctcagtg atcaagcttt tttatgcact gggaacagaa ctgatccctg
     2161 atgaagccta ttactgggaa tggtcaagaa ggcttgatct ttccttttac gatcagggac
     2221 ccggagtagg gctttacatc agactgttta cttcgatctt aggaaataca aattttgcgc
     2281 ttaaactggc agcagtatcg gcggggttcg gaacatctgt ctttctgatt tatacctcac
     2341 gccttctgca attcggcaaa aagggacttt tttggactgt cctgctttct cagctcattc
     2401 cgggattttt cggaggatct attttcatta tgcacgattc tcccctgctt ctgtcctggt
     2461 ctgccgcact gtattttact gtgcattata tattcaaaaa agatgtttta tcattatatc
     2521 tgatgttcct gtccctcggg ttcggggctc tatcaaaaca ctctatggtt ttctttgcac
     2581 tgagtctgat tgtatggctc attttaaatc ctaaacagtc cgcccttctg aaaaatcctc
     2641 atttttatgc cggtctgatt ctggcagggg cggtgttttc accggtcatc ctctggaata
     2701 ttcagcataa cagaatcagt tgatatgctg actgtggcag a
//
LOCUS       DAOQGJ010000330         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22902_length_2741_cov_3.749814, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000330 DAOQGJ010000000
VERSION     DAOQGJ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22902_length_2741_cov_3.749814"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PK683_06445"
     CDS             complement(<1..1147)
                     /locus_tag="PK683_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNH08123.1"
                     /translation="MIPLHLSFSGFNSYRTVQSVDFRKLLSSRLFGIFGETGSGKSSI
                     LDAMTLALYNTEKGKGTDSRNQNIMNLSSESMMIDFICDIGGKEYRFQVKAKRKKDGT
                     VDSLSRNVFEKSGGEWLKIRKTGEELLGISLSNFRKTVIIPQSSFQEFIELPPAKRTE
                     VLKELFSLDRFDLTEEVRKLLSSAKENQKILEGKILQLGSPDESVLSDKKNRLAAAET
                     SLEFLRRETQIKRTQLKEYELLMEKHEKKIQLEKEYEEILKQESSFLERKKNTERMIH
                     ISSEFRDISRRLNDEKKMLTDKTSLLKDLVKNIAYEEKQIQIERNGLEKLRSDKESAL
                     QEHSRIPQIRFLTEWKKKSAELEHSKKVLIQNAEKRKDNLRALNVLTE"
     gene            complement(1144..2370)
                     /gene="sbcD"
                     /locus_tag="PK683_06450"
     CDS             complement(1144..2370)
                     /gene="sbcD"
                     /locus_tag="PK683_06450"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HNH08124.1"
                     /translation="MSETLRILHTSDWHLGKHLGSFSRLPEQELVLEEICVLAEKNNA
                     ECIIVAGDIFDNYNPPVEAVQLFFRTVKRLSCEGKKPVLIIAGNHDSAERIESPSPLA
                     FENGIIFAGYNTTSFPLFSLESGLSLRKSEPGFAEFSLPGKKLPLRILFSPHIFSSAF
                     QKEISDERTLQEYLSETWKTSSEKNIIDKSYNILIGHFFMQPDSGNEQEESEEEKTVL
                     SIGGAERISPAAIPDKIHYAALGHLHRFQSVKGRDFPIIYSGSPIAYSLSESEQEKKT
                     VIADLDGTSAKISTINLKSGKRIFRKSFHSSQDAVLWLKENQNCLAEITIFTDRYLQG
                     SELREIRDSHSGILSVRPILSEASEEHIHSHIDMSKSVEELFIQFHTFRTSGLSPSSE
                     LMDIFREAAALKGTEK"
     gene            complement(2363..>2741)
                     /locus_tag="PK683_06455"
     CDS             complement(2363..>2741)
                     /locus_tag="PK683_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08125.1"
                     /translation="NKKLDNWDIFSNYLYFTARQTPGFIVRLNRNVGLKESKEFRESY
                     TAHYDFSGIKGRMEGIEFGDSWIGSFQYLGSMLKDRQRHLRLERNYPNLTTLPPLKLS
                     FKVSSSAGNISEDMEIALKAVYE"
BASE COUNT          659 a          590 c          566 g          926 t
ORIGIN      
        1 cctcagttag aacattgagt gccctcaggt tgtcttttct tttttccgca ttctggatca
       61 gaactttttt ggaatgttcc agttctgcag attttttctt ccattcggtg agaaaacgta
      121 tctggggaat tctgctgtgt tcctgcaatg cggactcttt atctgatctg agtttttcta
      181 gaccgttcct ctcaatctga atctgctttt cctcataggc aatatttttt acaagatcct
      241 tgagaagtga agtcttatcg gtaagcatct ttttttcatc attcagtctt ctgctgatgt
      301 ccctgaattc agaactgata tgaatcattc tttccgtatt tttctttctt tccagaaagg
      361 aagattcctg cttcagaatc tcctcatatt ccttttccag ttgaattttt ttttcatgtt
      421 tttccattaa aagctcatat tctttcagct gagttctttt aatctgagtc tctcttctga
      481 gaaattcgag acttgtttct gcagcggcga gcctgttttt tttgtctgag agaactgatt
      541 catccgggct tccaagctga agtattttcc cttccagaat tttctgattt tcctttgcgg
      601 aagaaagaag ttttcttacc tcttcagtca gatcaaatct gtccaggctg aacagctcct
      661 taagcacctc ggttcttttg gctggcggca gttcaatgaa ttcctgaaag ctgctctgag
      721 gtatgatgac tgtctttctg aaattgctca ggctgattcc cagaagttct tcacctgttt
      781 ttctgatctt cagccattca cctcccgatt tttcaaagac atttctgctc agactgtcta
      841 ctgtaccgtc ttttttccgt ttagccttga cctgaaacct gtattctttt ccgccgatat
      901 cgcagatgaa atcgatcatc atgctttctg atgataaatt cattatattc tgatttctgc
      961 tgtccgtgcc ttttcctttt tccgtattgt aaagagccag agtcattgca tccagaatgg
     1021 atgacttccc tgagcctgtc tcgccgaata tgccgaacag tcttgatgag agcagttttc
     1081 gaaaatccac agactgaact gttctgtagg aattgaatcc tgaaaaagaa agatgaagag
     1141 ggatcatttt tcggttcctt ttaatgctgc cgcttctctg aatatgtcca tcagttcaga
     1201 tgaaggagaa aggccggagg ttctgaatgt atggaactga atgaacagtt cctcgaccga
     1261 tttgctcata tcaatatgag aatgaatatg ttcttctgaa gcttcactga gtatcggtct
     1321 tacggaaagt atgccggaat gagaatccct tatttcacga agctcgcttc cctgaagata
     1381 cctgtccgtg aagattgtga tttcagccag acagttctga ttttccttca gccagagcac
     1441 tgcatcctga gatgaatgga aactctttct gaatatcctt ttaccggact tcaggtttat
     1501 tgtgctgatt tttgcagaag taccgtcaag atctgcgatg actgtttttt tttcctgctc
     1561 cgattcgctg agagaatacg cgattggact tccggagtaa atgatcggga aatctcttcc
     1621 ctttactgac tgaaacctgt gaagatggcc gagggccgca tagtgaattt tgtccggaat
     1681 ggctgcaggc gagatccttt cagcaccccc gatgctcaga actgtctttt cctcttcgga
     1741 ttcctcctgc tcattgcctg agtcaggctg catgaagaaa tgtccaatga gtatattata
     1801 tgatttatct attatatttt tttctgagga tgttttccat gtttcagaca gatattcctg
     1861 aagagttctt tcatctgaaa tttccttctg aaatgcactg gaaaatatat gcggagaaaa
     1921 aagtattctc agaggaagtt tcttgcccgg aagagagaat tctgcaaagc caggttctga
     1981 ttttcgcagt gaaagcccgg attcaagaga gaacagagga aacgaggttg tattgtatcc
     2041 tgcaaatatg attccgtttt caaaggccag gggagaaggg gattcaattc gttcagcact
     2101 gtcatgattg cctgcaatga tgagaaccgg ttttttccct tcgcaggaaa gtctttttac
     2161 cgttctgaag aaaagctgga cagcctctac gggaggatta taattatcaa atatatcgcc
     2221 ggcgactata atgcattcag cattgttttt ctctgcaagc acgcagattt cttcaagaac
     2281 aagctcctgt tcaggaagtc tggaaaaact ccccaggtgt tttccaaggt gccagtcact
     2341 ggtatgcaga attctgaggg tttcactcat acactgcttt taatgcgatt tccatatctt
     2401 cagaaatatt tcctgcagat gaggagactt taaagctcag tttaaggggc ggaagagtcg
     2461 tgaggtttgg ataatttcgt tccagtctga gatgtctctg tctgtctttc agcatacttc
     2521 ccagatactg aaatgagcct atccagctgt ctccgaattc gattccttcc attctgccct
     2581 tgatgcctga aaagtcatag tgtgcagtgt agctttccct gaattccttt gattccttga
     2641 gaccgacgtt cctgttcagg cgcacaatga atccaggtgt ctgtctggct gtgaaataca
     2701 gatagttcga aaaaatgtcc cagttgtcca gttttttgtt t
//
LOCUS       DAOQGJ010000331         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22903_length_2741_cov_3.319434, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000331 DAOQGJ010000000
VERSION     DAOQGJ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22903_length_2741_cov_3.319434"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK683_06460"
     CDS             complement(<1..539)
                     /locus_tag="PK683_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08126.1"
                     /translation="MLKLKAWFGTVIRKKKFWFFFAMLLIYKSVFNVFTGDYIFKKIF
                     SSASTGTIQINVKRFSLFFGVIAEDVVISSGEDFGKEPIFRTKRLAAAYNLPWLLLGR
                     LKLSEISLISPEIFLREKKGKWNIQTLFPPGKETPPPPASEPLGDEINLFVPVSAFAN
                     VFIRDLSLNAAKEKGKSYFS"
     gene            complement(539..1114)
                     /locus_tag="PK683_06465"
     CDS             complement(539..1114)
                     /locus_tag="PK683_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6521/LA_1396 family lipoprotein"
                     /protein_id="HNH08127.1"
                     /translation="MKYSILVLILSVFYCSTVKEVKRDNDLKGKLSGLKRIAVAVPSA
                     SKLTKMEQLFLAEVSKDQIAAKKDFIVFRSPDKFKGGCTKDNPKIEGVFQIDSVQEEK
                     GNSLKIQLNSSLINCRDGKTVWSAVSAGSFSADSRENESLRKTYTQKFGLGIESRVNP
                     YFRMINEMLEELESPVLNESEKDEKIEADSV"
     gene            1237..1764
                     /locus_tag="PK683_06470"
     CDS             1237..1764
                     /locus_tag="PK683_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002067859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNH08128.1"
                     /translation="MNENTDDSELKAPRKNLFSAKKIKYALGDRPKVDCILCGVKENN
                     PIVDNLVIAETEFSIVSVNLYPYNPGHLIIFPKRHLLNLTEFSDEEALDIHRLTVKAV
                     RIFSEKWRVQGFNIGYNMGKNSGGSIPHLHEHIVPRFPNESGFLDVLAETRVVIYDPF
                     DMKKEIQKMWNEFDT"
     gene            1815..>2741
                     /locus_tag="PK683_06475"
     CDS             1815..>2741
                     /locus_tag="PK683_06475"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH08129.1"
                     /translation="MLLQIKKAFLSILYYGTEEFSDPYEIRRTAISNILAIAGLTDMS
                     LVAGANLIFHNYKIIPVMILIYMMIGFYFLIIRFRKTDAARFFLMTTAVAVTGTFEIM
                     TRGRQGFGLHMFPILAGIIFLISLAALLGTGSRMDCLIRLTPDVTFSEELDRIIYVIG
                     LLSSLSITAASLVLLVHETGITDTRYREVIERIQEKNEFINIVTSSLPVFIYVYDIIR
                     KKTVYSNKSLSRYLGYEAEEFSFEEFMFKIHPEDVADVQTFFDGIFTSGNADYETEYR
                     LFGAVKDYIWFSASYRIFQSDEKGKTEKILVLF"
BASE COUNT          830 a          588 c          532 g          791 t
ORIGIN      
        1 gaaaaatagg acttcccttt ttctttggct gcattcagtg aaagatcgcg gatgaaaaca
       61 tttgcaaatg cactcacagg cacgaacaga ttaatctcat ctccaagcgg ctcagaagca
      121 ggaggcggag gcgtttcttt tccaggagga aaaagagtct gtatattcca ttttcctttt
      181 ttttcacgaa gaaatatttc aggacttatg agagatattt cagaaagttt cagccgtcca
      241 aggagaagcc atggcagatt gtaagctgcc gcaagtcttt ttgttctgaa aatcggttcc
      301 ttcccaaaat cttcaccgga agaaatcacg acatcttctg caatgactcc gaagaaaagg
      361 gaaaatcttt ttacattgat ctgaatcgtt ccggtggaag cagaggagaa tattttttta
      421 aaaatataat ctcctgtaaa aacattaaaa actgatttat aaatcagcag catcgcaaaa
      481 aagaaccaga acttcttttt tcgaatgact gtgccgaacc aggccttgag cttaagcatc
      541 agacagaatc agcctctatc ttctcatcct tttccgattc attcagaacc ggactttcca
      601 gttcttcgag catttcgttg atcatccgaa aataaggatt tactctgctt tcaattccaa
      661 gtccgaattt ctgagtatac gtttttcgca gactttcatt ctccctgctg tcggcagaaa
      721 aactgcctgc actcaccgca ctccatacag tcttcccgtc cctgcagtta atgagagagg
      781 aattcagctg tattttcaga ctgtttccct tttcttcctg aacagaatcg atctgaaaga
      841 ctccctctat tttagggttg tccttagtgc atccgccctt gaatttatca ggagaacgga
      901 aaacgataaa atccttttta gctgctatct gatccttgga aacctctgcc aggaaaagct
      961 gttccatttt ggtcagtttt gaagcagaag gcacggcaac ggctattctt ttcaaccctg
     1021 aaagctttcc tttcaaatca ttgtcccgtt tcacctcttt gacagttgaa cagtaaaata
     1081 cggataaaat taagactaaa atagaatatt tcatactccc ttatttcagc agagagcaga
     1141 gtgctgtcaa ctgttttcat cagttttgca ctgcctgtac gcagggtcta attaaaaaac
     1201 ttgcccaaaa acaattcagt ctggagagtc gatattatga atgagaatac ggatgattca
     1261 gaactgaaag cccccagaaa aaatctgttc tcagccaaga agataaagta tgctctcggt
     1321 gacagaccga aagtggactg cattctctgc ggagtaaaag aaaacaatcc gattgtggac
     1381 aatctcgtaa ttgcagaaac tgaattcagc atcgtttcag tcaatctcta cccgtacaat
     1441 cctggacacc tgattatctt tcccaaaagg catcttctga atctgactga attttccgat
     1501 gaggaagcac ttgacattca tcgtcttaca gtcaaggctg tccggatttt ttcagaaaaa
     1561 tggagagttc agggattcaa catcggatac aatatgggca aaaacagcgg cggttcgatt
     1621 ccgcatctgc atgaacacat cgtgccaaga ttcccgaatg aatccggttt tctggatgta
     1681 cttgcagaaa caagagtcgt gatctacgat ccgttcgata tgaaaaaaga aatacagaaa
     1741 atgtggaatg aatttgatac ttaaacaccc gctcaactct gaactgaaaa aattgtcaac
     1801 cccgaaaaca gcctatgctt ctgcagatta aaaaagcttt cctttccata ctgtattacg
     1861 gcactgagga attttctgat ccttatgaaa tcagaagaac cgcaatttca aacatacttg
     1921 caatcgcagg ccttacagat atgtcccttg ttgcaggtgc aaacttaatt tttcataatt
     1981 acaaaataat tccagtcatg attctcattt atatgatgat aggattttat ttcctgataa
     2041 tcagattcag gaagactgac gctgcccgtt tttttctgat gacgactgcc gtagcagtga
     2101 ccgggacttt cgaaattatg acaaggggaa gacagggatt cggtcttcat atgtttccga
     2161 ttctggcggg aatcattttt ctgatctctc ttgcagctct cctcggaacc ggaagcagga
     2221 tggactgtct gatccggctc actcctgatg tcactttctc ggaagaactg gacagaataa
     2281 tatatgtaat aggccttctc agctctcttt ccatcactgc agcatcgctt gtgcttctgg
     2341 ttcacgaaac aggaataact gacacaagat acagagaagt cattgagaga attcaggaga
     2401 agaatgaatt cataaacata gttacatcat ccctccctgt atttatatat gtttatgata
     2461 taataagaaa aaaaacagtc tactccaata aaagcctgag caggtatctg ggatacgagg
     2521 ccgaagaatt ttcattcgaa gaatttatgt tcaaaattca cccggaagat gttgccgatg
     2581 tgcagacttt ttttgacgga atattcactt caggcaatgc agactacgaa acggaataca
     2641 gactgtttgg ggcagtcaag gactatatct ggttttccgc ttcctaccgc atatttcagt
     2701 ctgatgaaaa aggaaagacg gaaaaaatcc ttgtgctttt t
//
LOCUS       DAOQGJ010000332         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22904_length_2741_cov_3.293001, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000332 DAOQGJ010000000
VERSION     DAOQGJ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22904_length_2741_cov_3.293001"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK683_06480"
     CDS             complement(<1..343)
                     /locus_tag="PK683_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH08130.1"
                     /translation="MGIFDKIKGQFVDVIEWVDNSSDTLVYKFDRKDNEIKNGAQLTV
                     RESQVAVFVNEGKLADIYKPGRYELTTNNMPILTTLKSWKHGFDSPFKVDVYFINTKT
                     FTGQKWGTKNPI"
     gene            complement(511..966)
                     /locus_tag="PK683_06485"
                     /pseudo
     CDS             complement(511..966)
                     /locus_tag="PK683_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013853.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
     gene            1113..>2741
                     /locus_tag="PK683_06490"
     CDS             1113..>2741
                     /locus_tag="PK683_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08131.1"
                     /translation="MEKLLEHIDQIQGTGSFCTGGILDSCMPGISIKGYGEISLPLQE
                     KELPNLEKFFSPAPYGKGEETLVDRNVRSTKELNPERFSLNNPEWKTSMDKAVQETAL
                     RLGLGKSKINYSLCKLLLYSEGDFFLPHRDTEKETGMFATMVIALPSVHSGGDLLVTH
                     NGRTEKFSFSSRKYLYQAGYAAFYADCEHEVGRIKKGARLCLVYSLKMADLKTPPDLQ
                     SQSYQIELAEALLRKARMKTDKAFFLLEHKYTQRSFSKKSLKSSDIARAEILFQSAEK
                     TGYSAHLALVTLNRTHDLQSNYDDFFEVDEAGGLIDSVLTADCWFDSEGRPASFGKMG
                     IEESEIYSSRPFGEGEADETEKEEYTGNAGATVDLWYRRAAVVLWKKKKHFSILAKGL
                     DRDAAGALLLKHLKSSSSEYDREDLISFAYDIIKEWKQDLLSGSVSLQNRMLSSLNIL
                     SDADLTKEFFREVLAFSYGKGSAKNISESLYHHGIENFNDELRIFVRADSDKLKTDFP
                     ELLFYLVQSAEKSPKGMKTCRGLGRDYLSSLSKAD"
BASE COUNT          763 a          544 c          610 g          824 t
ORIGIN      
        1 gaatagggtt ttttgtgccc catttctgtc ctgtaaacgt ttttgtattg atgaaataca
       61 catctacttt gaatggagag tcaaatccgt gcttccagct ttttagtgtg gtcaggatcg
      121 gcatattgtt tgtagttaat tcataccttc caggcttgta gatatctgca agttttcctt
      181 catttacaaa aactgctact tgggactctc tgactgtgag ctgtgcaccg ttcttaattt
      241 cgttgtcttt gcggtcaaat ttgtagacta gagtgtcgct ggaattatcc acccactcga
      301 tgacatctac aaactgccct ttgattttgt cgaatatgcc cattccgaac tcctaagtta
      361 aaattcttac aaatactgtc agctcctgat aatggaactt cgtccaaaaa actcgtccgg
      421 catttgcacg acggttttca gtaaatgcag gatgcatgat atccgttaga ctgttttttt
      481 ccagtacttt ttaaaaaaaa taggaatgtt tcagttttcc ggccagtaat tgtcatcgat
      541 ggactcttcc aaagaaaacg ggaggtcttt tggaaattta ttttcaggaa tcttcgtttc
      601 caggcttgct ctccttttgg ctttatcata agcccggtca gtgttttcct cagcatactt
      661 ctttagattt tggctgtccg aaaaaatttt gttcagttca tctctctgct tttgaatgga
      721 aacttaccag ctgcttcccc tgtaatcttt ctgcgtcatc catttcaata gatgaagaaa
      781 cagttcaatc atcctggaaa acacttctct gaactgagat tttctcataa tttcgagttc
      841 ctccgcaatg tgttcccagt ctgcttctga aaatcttcca ttccgcagaa gctgaatatt
      901 ttctataatc cagagacttt cgtcctttgt atacagctct ttcagtgtga ttgtcttggc
      961 agtcatgacc ttatgatagg tgaacataca tttgagtcaa gcagcaatcg gtttttctga
     1021 ccctgaaaaa atggaaaatg agtttaagat atttcattgt attctgatat tatccaaatg
     1081 aactgctcct gcaggctgaa caaaggattt atatggaaaa acttctggaa cacatagatc
     1141 agattcaagg cacaggctcc ttctgcaccg gaggcattct ggacagctgt atgcccggga
     1201 tcagcataaa aggctacggt gagatttcac tgccgcttca ggaaaaagaa ctgccgaatc
     1261 tggaaaagtt cttttcacct gctccttacg gaaaaggtga agaaactttg gtggacagaa
     1321 atgtcagaag cacaaaagag ctgaacccgg aaaggttttc tctcaacaat cctgaatgga
     1381 aaacttcaat ggacaaggct gtgcaggaga ctgcacttcg gctggggctg ggaaaaagta
     1441 aaattaatta cagtctatgc aagctgcttc tttattctga aggcgatttt tttcttcctc
     1501 acagggatac tgaaaaagag acagggatgt ttgccactat ggtgattgcg cttccttcgg
     1561 ttcacagcgg aggcgattta ctggttacac ataacggcag aacagagaag ttctccttca
     1621 gttcacgtaa atatctttat caggcaggtt atgctgcttt ttatgcagac tgtgaacacg
     1681 aggtcggcag aattaaaaag ggagcaaggc tttgtcttgt atacagtctg aaaatggcag
     1741 acttgaaaac accgcctgat ctgcaaagcc agtcatacca gattgaactg gctgaggctc
     1801 tgctcaggaa agccagaatg aaaacagaca aggccttttt tctgctggag cataagtaca
     1861 ctcagagaag tttttcaaaa aaatcgctga aaagttctga tattgccaga gcagagattc
     1921 tgtttcagtc tgcagaaaaa acaggttatt cagcacatct ggcacttgtc actctgaaca
     1981 ggactcatga ccttcagtca aattatgatg attttttcga agtggatgaa gcaggcggtc
     2041 tgatagattc tgttctgact gcagactgct ggttcgattc tgaaggaaga cctgcttctt
     2101 tcggcaaaat ggggattgag gaatctgaaa tctattcttc caggccgttc ggagagggtg
     2161 aggcggatga gacggagaag gaagagtata caggaaacgc gggagccact gtagaccttt
     2221 ggtacagacg ggctgcggtt gtactgtgga agaagaaaaa gcatttttcc attcttgcaa
     2281 aaggtctgga cagggatgct gccggtgcac ttcttttaaa gcatctgaaa agcagttctt
     2341 ctgaatatga cagagaggat ctgatcagtt ttgcatatga tatcattaag gaatggaaac
     2401 aggatcttct gtcaggctca gtcagtctgc agaaccggat gctcagttca ctgaatattc
     2461 tttcagatgc agacttgaca aaggagtttt tcagagaggt tcttgcattt tcatacggga
     2521 aaggttctgc aaaaaatatt tctgaatcat tatatcatca tgggatagaa aattttaatg
     2581 atgaattgag gatttttgta agagctgatt ccgataaact gaagactgat tttccggaac
     2641 tgctttttta tctggttcag tccgcggaaa aaagtccgaa ggggatgaag acctgcaggg
     2701 ggctcggcag ggactatctt tcctcacttt ccaaggcaga c
//
LOCUS       DAOQGJ010000333         2740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22928_length_2740_cov_2.848045, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000333 DAOQGJ010000000
VERSION     DAOQGJ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22928_length_2740_cov_2.848045"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PK683_06495"
     CDS             <1..871
                     /locus_tag="PK683_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HNH08132.1"
                     /translation="EGLLTALLKEDITILNLLETEGNQEEETDKFNRVDLLVEDSQKR
                     KFIIEVQNNRETDYLERLLYGTSKTITESVNLGKGYSDIAKVISVSILYFNLGKGDDY
                     IYYGRTDLLGVHTNHPLVVKEQVQKAEDFEIKIRLQQKNIFPEYYLIHTEKFENVISS
                     PIDEWIYLLKNSEVKENFRSKNIDKAREKLRILNMTSEQRKQYERFLINLASEKDVIE
                     TAREEGKLKGEQIGLEKGEQIGLEKGEQIRIKKEDTAIENALRKGKLSPEEISEMLEV
                     SLERVLEVKKNIE"
     gene            1177..2121
                     /gene="trxB"
                     /locus_tag="PK683_06500"
     CDS             1177..2121
                     /gene="trxB"
                     /locus_tag="PK683_06500"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNH08133.1"
                     /translation="MAEKVVIIGSGPAGHTAAIYAARANLSPLMFEGFMAGGIAAGGQ
                     LTTTTEVENFPGFPEGIDGTKLTQLFREQSEKYGTRIKTETVTKVNFKVHPFKIYTDE
                     GEVEAETVIIATGATAKRMHVPGEDSYWQRGISACAVCDGALPIYRNKPLAVVGGGDS
                     AIEEACHLTKFGSKVYLIHRRDRLRASQIMQKRAVNNPKIEILWNSAVEEAKGDGKSL
                     TSIVLTDTVNGTKRQLETGGLFYAIGHVPNTEVFKDFLELDETGYIKTVPGTTKTNIP
                     GVFAAGDVQDKVYRQAITAAGSGCMAALEAEKFLSEKE"
     gene            2121..>2740
                     /locus_tag="PK683_06505"
     CDS             2121..>2740
                     /locus_tag="PK683_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08134.1"
                     /translation="MIKKLIARFFPLLLLFFANCFDYEEVIYFRKPFSGYVEITYTVP
                     VHPRTGKSLLRFLPLTSEEIEKKINKGIFSKNLKIREHTLTYVEKTENDSVSFYQKKA
                     RVNYKLDFEDLNKLDGILLGYLFVKKKGSSLIVKREFKSVLKGLDQNSTTGEKQIHAE
                     TLKLLGDSSILFKVIFPNNWECRSSKGEIFLGSLVYRFPLSETVDKP"
BASE COUNT          915 a          538 c          620 g          667 t
ORIGIN      
        1 cgaaggactt ctcactgctc tcctgaaaga ggatattaca attctgaatc ttctggaaac
       61 tgaaggaaac caggaagagg aaacagacaa attcaacaga gtggatcttc tggtcgaaga
      121 ctctcagaaa agaaagttta tcattgaggt gcagaacaac cgcgagacag attatctcga
      181 aaggcttctt tacggcactt ctaaaacaat tacagaaagc gttaatctgg gaaagggata
      241 ttctgatatt gcaaaggtta tttctgtcag tatactgtat tttaatctcg gcaaaggtga
      301 tgactatatt tactacggca gaacggatct tctcggagtg cacacaaatc atcctcttgt
      361 ggtcaaggaa caggtccaga aagcagagga ctttgagata aagatcaggc ttcagcagaa
      421 aaatatattt cccgaatact atctgattca cacggaaaag tttgagaatg tgatcagctc
      481 accgatagat gaatggattt atcttctgaa gaacagcgaa gtgaaggaaa acttcagatc
      541 aaagaatatt gacaaggcca gagaaaaact tcgcatcctg aatatgactt ctgagcagag
      601 aaaacagtat gaaagattcc tgatcaacct ggcctcagaa aaggatgtga ttgaaactgc
      661 cagagaagaa ggcaagttga aaggcgaaca gatcggtctt gagaaagggg agcagatcgg
      721 tctggaaaaa ggtgaacaga tcaggataaa aaaagaagac actgccattg aaaatgctct
      781 ccgaaagggg aaactttccc ctgaagaaat ttcggaaatg ctggaagtct ctctcgagag
      841 agttctagaa gtaaagaaga acatagaatg aattgtcttc tgctgcattc ggcaggctct
      901 gtgtgagtat tatcttttat ttacttttac aaaccctcaa tccctcgtat atattatata
      961 atgaaattca atacagaaag gcagtgctgc ccccacacct gtttacaaaa tagaaaagca
     1021 gccttatcgc agcactttcg aactcttccc ctctcccctc ctaaaaaatt tctctgcaga
     1081 ttacagccgc actgcttcct tacttcacct gcgaggagag aaaatttttc ttgatctgtg
     1141 atctttgaca gaaaaacgta gaaggaggag agataaatgg cagagaaagt agtaatcata
     1201 ggatcaggac cggcaggaca cactgcggcg atatatgcgg ctagagcaaa cttgagtccg
     1261 ctgatgtttg aaggatttat ggccggagga attgcagcag gagggcagct gaccacaaca
     1321 accgaggtcg agaattttcc agggtttcct gagggaattg acggaacaaa actgactcag
     1381 cttttcagag aacagtcgga aaaatacggt acaagaatca aaacagaaac agtcacaaaa
     1441 gtcaatttca aagtgcatcc ttttaaaata tatactgatg aaggcgaggt ggaagcagag
     1501 acagtcatta ttgctacagg agcaactgca aaaagaatgc atgttcccgg cgaggacagt
     1561 tactggcaga gaggcatttc tgcatgtgcg gtctgcgacg gagcacttcc aatatacaga
     1621 aataaacctc tcgcagttgt gggcggagga gattctgcaa tagaagaagc ctgtcatctg
     1681 actaagttcg gatcaaaagt ctatctgatt cacagaaggg acagactgcg tgcatctcag
     1741 attatgcaga aacgagctgt caacaatccg aagattgaaa tcctctggaa ttctgccgtg
     1801 gaagaagcaa agggagacgg aaaatccctg acatcaattg tgctgacaga cactgtgaac
     1861 ggaaccaaaa gacagctgga aacaggcgga cttttttatg caataggcca tgttccgaat
     1921 actgaggtct tcaaggattt tctcgaactc gatgaaacag gatatattaa gacagttccc
     1981 ggaaccacaa agacaaatat tccgggagtt tttgcagcag gtgatgttca ggacaaggtt
     2041 taccgtcagg cgatcactgc agcaggttcg ggatgcatgg cagccctcga agcagagaaa
     2101 tttctttctg aaaaagaata atgataaaaa aactcatagc aagattcttc cctctcctgc
     2161 ttcttttttt tgcaaactgt tttgactatg aggaagtgat ttatttcaga aagccttttt
     2221 ccggctatgt agaaattaca tacactgtgc ctgttcatcc cagaacaggc aaatccctgc
     2281 tcaggtttct tcctttgact tccgaagaaa tagaaaaaaa aatcaacaag ggaatttttt
     2341 ccaagaacct gaaaatcagg gagcatacat tgacctatgt ggaaaaaaca gaaaacgatt
     2401 cagtttcctt ttatcagaaa aaggccaggg tcaattacaa gctggacttt gaagatctga
     2461 acaagctcga cggaatactt ctgggatatc tcttcgtgaa aaagaaagga agttcactta
     2521 tagtaaaaag agaattcaaa tcagttctga aaggactgga tcagaattcc actacaggag
     2581 aaaagcagat ccatgcggaa actctgaagc ttctcggcga cagttccatt ctttttaaag
     2641 tcatctttcc gaacaattgg gaatgcagat cctccaaggg tgagattttt ctgggatcac
     2701 tggtgtacag atttccgctc tctgaaacgg tggacaaacc
//
LOCUS       DAOQGJ010000334         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22958_length_2738_cov_3.695863, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000334 DAOQGJ010000000
VERSION     DAOQGJ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22958_length_2738_cov_3.695863"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PK683_06510"
     CDS             <1..1113
                     /locus_tag="PK683_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNH08135.1"
                     /translation="GKIRTAILSDPDWLGEDFPALEKKLKEKGHFTIRQKITDKVIGE
                     FVLKHIELEKILEKGKKKEFHVVLLGKLQLRQKSYSKKISNDFYIAMHKTEDAYLDPS
                     VFYKDEPTPPASSLIHPKDKKEMVLVPAGLFMHGQGTDGSGDNYNPAFQAPAHHNVME
                     LPSFYIDKYEVTNREYDRYLEETGASPPKHWPGGEIPSGKEDHPVTSLTYREAEGYAK
                     WAGKRLPTEFEWEKAARGSGITLIKNRDESFTFDVHTVKYPWGNKFNSSFCNSAESGK
                     KDTVSVYELSEKGASPYGVIGLCGNAPEWTSSWYEPYEGHNFKSSVKGNAVKVIRGGS
                     FIDSAKNCTVYFRNYGGMPNLSEDRRAGFRLVKDLN"
     gene            1116..1361
                     /locus_tag="PK683_06515"
     CDS             1116..1361
                     /locus_tag="PK683_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08136.1"
                     /translation="MNDTSPEIEQMLQERYMKMTGEERVMIATSMYDTAREIVLSSLP
                     PNLTEKEKRVQLFLRFYGNEFSDEGREKIVNHLMNVD"
     gene            1367..2131
                     /locus_tag="PK683_06520"
     CDS             1367..2131
                     /locus_tag="PK683_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08137.1"
                     /translation="MLYFLQMLQMNFFFLVSAFAAAICVSMEIGAVYSVLTGIALGFL
                     FGYFAMTVDDEKPFKLLENQSDNYSVILSVYLTAVSFIGAVYTVGKFYSYFECDDKAL
                     TVSFDQLKVPQKKKFIHLENYYARPVKDLNLYKSNILYQKYQITELLSDSVKQERTNI
                     WLVCEGCSEEEARHLMNGKNAYLRTAAEDLSGFLPQLDPDAERDSAIFYILLRGNLEE
                     ERNAILWDFYRAAGYANLGFFLIVLILVICRKLGMD"
BASE COUNT          833 a          551 c          607 g          747 t
ORIGIN      
        1 ggaaaaatca gaactgcaat tctgtccgat cctgactggc tgggtgaaga ctttcctgcc
       61 cttgaaaaaa agctgaaaga aaaaggccat tttacaattc gtcagaagat tacggacaag
      121 gtgatcggcg aattcgttct gaaacatatt gaactggaaa aaattctgga aaaagggaaa
      181 aaaaaagaat ttcacgtagt tcttttggga aaactgcagc tgaggcagaa atcctattcc
      241 aaaaaaatat caaacgattt ctatattgca atgcacaaga ctgaggatgc ctatctggac
      301 ccttcggtat tttacaagga tgaaccgacg cctcccgcat cttcattgat tcatcccaaa
      361 gataaaaagg aaatggttct ggttcctgca gggcttttta tgcacggtca gggcacagac
      421 ggaagcggtg acaattacaa ccctgcattc caggctcctg ctcatcacaa tgtaatggaa
      481 cttccttcat tttatataga taaatatgaa gttacgaaca gagaatatga cagatacctc
      541 gaggaaaccg gagcatcccc gccaaaacat tggcctggcg gagaaatacc ttccggaaaa
      601 gaagaccatc cggtgacatc cctgacctac agagaagcgg aaggatatgc taaatgggca
      661 ggaaaaagac ttcctacaga atttgaatgg gaaaaagcag cgagaggatc aggaatcact
      721 ctgatcaaga acagggatga atcatttaca tttgatgttc atactgtaaa gtatccttgg
      781 ggaaataaat tcaattcctc cttctgcaat tctgcggaga gcggaaagaa ggatactgta
      841 tccgtatatg agctttccga aaaaggagca agcccatacg gagtgatcgg gctctgcgga
      901 aatgcacctg aatggacaag cagctggtat gagccgtacg aaggccacaa cttcaaatcc
      961 tcagtcaagg gaaatgcagt caaagtcatt cgcggcggtt ccttcattga cagtgcaaaa
     1021 aactgtacag tgtacttcag aaattacgga ggaatgccta atctcagcga agacaggaga
     1081 gcgggcttcc ggctcgtcaa agatctgaac tgagaatgaa tgacacatca cccgaaattg
     1141 agcagatgct tcaagaacgt tatatgaaga tgaccggcga ggaaagagtg atgattgcta
     1201 cgtccatgta cgacactgca agagagattg ttctgtcttc tctgcctccg aatctgaccg
     1261 agaaagaaaa gcgggttcag ctttttcttc gattttacgg gaatgagttt tctgatgaag
     1321 ggagagaaaa aatagtaaat caccttatga acgtggactg aaaaatatgc tctattttct
     1381 gcagatgctt cagatgaatt tcttttttct agtttctgct tttgcagcgg cgatatgcgt
     1441 ttcgatggaa atcggagcag tgtattcagt actaaccggc atcgctctcg ggtttctttt
     1501 cggatatttt gcgatgacag tggacgatga aaaacccttc aaactgctgg aaaatcagtc
     1561 ggacaattat tcagtcattc tttccgttta tctgaccgca gtttccttta taggagcagt
     1621 gtatactgta ggaaaatttt actcctattt cgaatgcgac gacaaggcac tgacagtgag
     1681 ttttgatcag ctgaaagtcc ctcagaaaaa gaaattcatt catctcgaaa actattatgc
     1741 acgtccggta aaagatctga atctttataa gtcgaatata ctgtatcaga aataccagat
     1801 cactgaactg ctttctgatt ctgtaaagca ggaaaggaca aatatctggc ttgtatgtga
     1861 ggggtgttct gaggaagaag caagacactt gatgaacggg aaaaatgcgt atctcagaac
     1921 tgcagctgaa gatctttccg gttttcttcc tcagctggat ccggatgcag aaagagactc
     1981 tgcaatattt tatatccttc ttcgcggaaa ccttgaagaa gagagaaatg caatactctg
     2041 ggatttttat agagcagcag gatatgcgaa tcttggattc ttcctgattg tattgattct
     2101 tgtcatttgc agaaaactgg gaatggactg attattccct gctattgcag tcctgtcggc
     2161 ggaaatccag tcccattcat agactgaaat tacactttct atatcactga atgtcgcctt
     2221 gacggccgcc gagcaatgct gtacaattaa cagtagacgt tcaaagatgt tatgaggaat
     2281 gatgcctcga tacaattttc accgccggtc accgagtgaa aaacccaata ttttcagggt
     2341 ttttcttatc gaggtgggcg gtgaaaatca ctcggcaacc gtctttcctg aataaagaac
     2401 cttaatttga cagcattggg ctgctgagac cttccagatt ttcagccggt attcttcggg
     2461 acaggagtgg ctgaatgcct gatgaatttt ccgcctgcta gaagcagaaa taactgttca
     2521 tcccctcatt cagttcctgc agagtttcat aagatatttt ataaatatta ctcagatttc
     2581 taaacccgca agcattgacg cagctatccc tgctttttct caccttctgt gtcgacaaga
     2641 atttactttt agcaagttct ttattttata attataatgt aattaaaaaa tattagtatt
     2701 gcatgcttca gggtgtccct tggtctttca gagaattg
//
LOCUS       DAOQGJ010000335         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_22961_length_2738_cov_3.447633, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000335 DAOQGJ010000000
VERSION     DAOQGJ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22961_length_2738_cov_3.447633"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..208)
                     /locus_tag="PK683_06525"
     CDS             complement(5..208)
                     /locus_tag="PK683_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08138.1"
                     /translation="MNESLLSERLEKIIQIRPDMENSVLKLKEWIIQSDILEKYRINP
                     YRLVPLLALEITTTRISIFGYAV"
     gene            782..>2738
                     /locus_tag="PK683_06530"
     CDS             782..>2738
                     /locus_tag="PK683_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08139.1"
                     /translation="MRFLQTLTACIIFTAVSFCSGKNGNKDFWKDNLFVDGLNAVSGL
                     SFFNLLDGYPVIKSGFKSLSPVDFNARLNAALQNIHRNDVTGALRAVQGTLLEGRSNV
                     QNTLLNVSEILTRIKTADSSSYDRLNEYIEKLRNYSKPILRGLMPISAEFLLSEYASK
                     NSETLKTRINSFASELQSQNVKDLFTKGEDITYKALVTNTTVRAAIESVMNAFMDASI
                     IKDSTFRSSVAGILYGVGDMMNVKAGFSDTKSPETVFKELIINLEKYYTVGGSSYTTE
                     YSNASFSSEFAQALTELYTEVKKLIITPTSVTKDTTNPTNTSEPLILANELAKNLALF
                     DFPATISGVDQSLKDLIRLDFKGRDRSYSNNGSIEISALETLVFVLALVDSFGYIWEN
                     AVSCNANAGCTNQITGSTGGVMRVGDTLWALQSTIRSSDTLNFKNILNNNATSRAVYK
                     GGVELSTNTSKAVDINAEVLRLLEAGSLGAVKSITAGGTDPVYSKTIPWVMNWIKKVV
                     YEGYGPYYNKNKKNSAGQFLNPDGSVYNANASDTNAKYVSSWKTADYKICVDKSGGNR
                     RWVGLGGYESNGTTIPHPTGLCNAGTNTPPSSAVADWSYTIYEMSKTDAERAVETDEE
                     AFYKNFQWLLYEKRFVVIIPAKAKLDAS"
BASE COUNT          858 a          571 c          570 g          739 t
ORIGIN      
        1 tattttacac agcgtacccg aagatcgata tccttgtggt agtaatttcc aaagcaagca
       61 gaggaacaag tctgtaagga ttgattctgt atttttccag aatatcggac tggatgatcc
      121 attccttcag tttcaggact gaattttcca tatcaggtct gatctgaata attttctcca
      181 gacgttcaga caaaagagat tcgttcattt ttcatttatg actgataaaa tgaacacagt
      241 caatttcttt ttcagtattg tttatgagag aaagaatggt aacgacagta gatttctctt
      301 ataaaaaatc tgagactgcg aaaaaaaatc atgaccgtga attcagtatc cttcccctgg
      361 attgaatgca gaattcattt ttttcataga aatcagcata tataatgact ttccttgaac
      421 cgcacggctt tttttcggct taaaacatat caaatatctt gaagtcggcc gccctccggc
      481 ttgaaaaccg ctgctgttat tgatttatat ggaacttctt taaggttttt taaaaattac
      541 aggctccagc attgtattgt ttttgcgtag gctgaccgat cagcccttac tcagtagttt
      601 tctcgaaaat gaaattctat tccgttcctg caggcagacg aaaataatct gaaaatcttt
      661 agaaaaaaat tcttgctttg gaaaaaattc tattgtcaaa tgtagatatt tttgttacat
      721 tgctatccca gaagccggtg tttaacaaac accgttcaca agggaaaacg gagaattgga
      781 aatgagattt ttacaaacac tcaccgcctg cattatattc acagcagttt ctttctgttc
      841 aggaaaaaac ggaaacaagg atttctggaa agacaacctg ttcgtggatg gactaaatgc
      901 agtcagcgga ctcagttttt tcaacctttt agacggttat cctgtcatta aatcagggtt
      961 caaatctctg tccccagtcg attttaatgc aaggctcaat gccgctttgc agaatattca
     1021 cagaaacgat gtaacaggag ccctccgtgc cgttcaaggc accctgctcg agggcagaag
     1081 caatgttcag aatacccttc tcaatgtctc cgaaattcta acaaggatca agactgccga
     1141 ttcgagttca tatgacagac tcaatgaata tatagaaaag ctgcgaaatt attccaagcc
     1201 tatccttcga ggactgatgc cgatttctgc agaattcctg ctcagtgaat atgcttccaa
     1261 aaattcagaa actttaaaga caaggatcaa cagttttgca tcggaactcc aatcacagaa
     1321 tgtcaaggat ttgtttacaa agggcgaaga tattacctat aaagccttgg tcaccaatac
     1381 cactgtcaga gctgcaatag aatccgtaat gaatgcattt atggatgcca gtatcatcaa
     1441 agactcaacc tttcgctcaa gtgttgccgg aattctttac ggggtcgggg atatgatgaa
     1501 tgtaaaggca ggcttcagcg acacaaaatc ccctgagact gtgtttaagg aactgatcat
     1561 caacctggag aaatactata cagtgggagg gagttcctac acgacagaat acagcaatgc
     1621 aagcttttcc agtgaattcg ctcaggcact cacagagctt tatacagagg tcaaaaaact
     1681 gatcatcaca ccgacatcgg tgacaaagga cactacaaat ccgacaaata catccgagcc
     1741 tctgattctc gcaaatgaac tcgctaaaaa tctggctctt tttgatttcc ccgccacaat
     1801 ttccggagta gaccaaagcc tgaaagacct gatccgtctg gacttcaaag gaagagacag
     1861 atcctactcc aacaatggaa gcattgagat ttctgctttg gaaaccttgg tttttgtgct
     1921 ggctctggta gattccttcg gatatatttg ggaaaatgca gtctcctgca atgcaaatgc
     1981 cggctgtaca aaccagatta ccggatccac tgggggtgta atgagggtag gagatactct
     2041 ctgggcactt cagtccacaa tcagaagttc tgatacactg aattttaaga atattctgaa
     2101 caacaatgcg acaagccgtg cagtttataa gggaggggtg gaactgtcca cgaataccag
     2161 caaggcggta gacatcaatg cagaagtgct ccgtcttctg gaggcaggtt cactgggtgc
     2221 tgtcaaatca attacagctg gcggaacaga tccggtctac tccaaaacaa ttccctgggt
     2281 aatgaactgg atcaaaaaag tcgtctatga aggttatgga ccttattaca ataaaaataa
     2341 gaaaaacagt gcaggacaat ttctgaatcc tgacggatcg gtatataatg caaatgcaag
     2401 tgatacaaat gcaaagtatg tatccagctg gaaaacagct gactataaga tctgcgtgga
     2461 taaatccggc gggaacagaa gatgggtagg cttgggagga tatgaaagca acggaaccac
     2521 aatcccgcac ccgactggac tctgcaatgc aggaacaaac acccctcctt cttctgctgt
     2581 ggcagactgg tcgtatacaa tttatgagat gtctaaaacg gatgcagaac gtgccgtgga
     2641 aacagacgaa gaggcatttt ataaaaattt ccaatggctt ctgtatgaaa agaggtttgt
     2701 agtcatcatt ccagccaagg caaaactgga cgcatctc
//
LOCUS       DAOQGJ010000336         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23132_length_2726_cov_2.842007, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000336 DAOQGJ010000000
VERSION     DAOQGJ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23132_length_2726_cov_2.842007"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1204
                     /locus_tag="PK683_06535"
     CDS             74..1204
                     /locus_tag="PK683_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNH08140.1"
                     /translation="MKLGDFFSGQYVNRSQYKSFEPSKIHLPWVIDDSELMMLLSEAD
                     IKLGELNGISELVPDVNSFLQMHVLKEGTESSRIEGTQTNIEEALQKEGDISPEKKDD
                     WQEVQNYVLALNSAIRQLAEIPISGRLLKNTHRILMQGVRGKNKMPGEYRETQNWIGG
                     ASLSDAVFIPPHQNSVSELMTDLEKFLNDENLRVPKLIKIGIAHYQFETIHPFLDGNG
                     RVGRLLIPLYLVENRILNKPVLYLSDFFEKNKSLYYDNLMRVRTHHNLVQWLKFFLVG
                     VRQTSQNSIDTFRSILAVRKKTEEKILTLGRRIPTAKKLLQFLFLKPVADSQEIEEAI
                     QMNATTAFRIIDEFLRLEILREKTGNKRNRIFIFEDYLGLFR"
     gene            complement(1197..1472)
                     /locus_tag="PK683_06540"
     CDS             complement(1197..1472)
                     /locus_tag="PK683_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08141.1"
                     /translation="MDKIQEILGMASRLKTVLKEKYKILPQDISSLKIDGDNFKVDIT
                     TKKNLMIRIKAKGTDTVLASNPDNGTMVMQQDPALAQCLFRIADEFT"
     gene            complement(1911..>2726)
                     /locus_tag="PK683_06545"
     CDS             complement(1911..>2726)
                     /locus_tag="PK683_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH08142.1"
                     /translation="DTVIKSTENYITADIRMRKGGSFRWKKLDIYRFKGIDGISTDTV
                     ITSIDMNELVMLRKYNETRIANNQLIDILTSVVESRNLESGNHIQRIRTVTRLLLEQI
                     MVDFPEYELTLELIDIISSASALHDIGKIAIPDSILLKPGKLTDEEFEIMKGHTIKGC
                     EIINSAAAIQDQEYYKYCYDICRHHHERYDGSGCPDGLRGDNISLAAQTVSLADVYDA
                     LISRRCYKEAFDEEKVFTMITQGESGIFNPKLLKAMNRIRPELKSSYLSIQPK"
BASE COUNT          730 a          529 c          599 g          868 t
ORIGIN      
        1 gaatatagga aaaagtaatt gtggttttgg ttttaaatgc aagtatggaa ttctgtaatt
       61 gcaggaggaa tatatgaaac tgggagattt tttttcaggc caatatgtga accggtccca
      121 atacaaaagt tttgaacctt cgaaaattca tctcccttgg gtaatcgatg attccgagct
      181 gatgatgctc ttgagcgaag cggatattaa gctgggggaa ctgaacggga tctccgagct
      241 ggttccggat gtgaattcct ttctgcagat gcacgttctg aaagaaggga cggaaagcag
      301 caggattgaa ggaactcaga caaatattga agaggctctt cagaaagagg gggatatttc
      361 ccctgaaaag aaggacgatt ggcaggaagt ccagaattat gttctggctc tgaattctgc
      421 gatacgacag cttgccgaga ttccgatttc cggaagactt ctgaaaaata ctcaccggat
      481 tctgatgcag ggggtccgag gaaaaaacaa gatgcctggg gaatacaggg aaacccagaa
      541 ctggatcgga ggggcctctc tttcggatgc cgtttttatc ccacctcacc agaactctgt
      601 ctccgagtta atgacggacc tggaaaagtt tctgaatgat gagaatcttc gtgttccaaa
      661 attgattaaa atcgggatag ctcattacca gtttgaaaca attcaccctt ttctggacgg
      721 gaacgggaga gtcgggaggc ttctgatacc tctgtacctg gtggaaaaca gaattctgaa
      781 taaacctgta ctgtatcttt ccgatttttt tgaaaagaac aagtctcttt attatgacaa
      841 tctgatgagg gtcagaactc atcataattt ggtgcagtgg ctgaagtttt ttcttgtggg
      901 agtcaggcag acttcccaga attccattga tacattccga tccattctgg ctgtcagaaa
      961 aaaaacggaa gaaaaaattc tcacgctcgg aagaagaatt cctacagcca aaaagctgct
     1021 tcagtttctg ttcctgaagc ctgtggcaga ttctcaggaa attgaagagg caattcagat
     1081 gaatgcgact actgcttttc ggattatcga cgagtttttg agattggaaa ttttgagaga
     1141 gaaaacaggg aataaacgaa acaggatttt tattttcgaa gattatttag gtcttttcag
     1201 gtgaattcat cagcgattcg aaatagacat tgtgccagtg cagggtcctg ctgcatcacc
     1261 atggttccat tgtctggatt ggaagccaga acagtatcag tgccttttgc tttgatccgt
     1321 atcatcaggt tttttttagt ggtgatatct actttaaaat tgtcaccgtc aattttcaga
     1381 gaagaaatat cttgaggaag aatcttatat ttctccttaa gaacagtctt cagtcgggag
     1441 gccattccta atatttcctg gattttatcc atgtcttcaa aagtcttgtc agccatttac
     1501 gctgatattg ctcaaaatgc tgattctgtc aacagttttt ttccaatgct taattgttca
     1561 actgtcatag gttcctgtta cattctgttt cagtagaaat gatacagttc ttcgatatct
     1621 gtgttttgaa attcagtatt tcctctctca gttcttttgc ttggcaaaat gatacagcag
     1681 cctttactgg gtcactgcat tggaattttt actgaaccaa agggtatact gacctgtctg
     1741 acttcttcta tcaattctgc ctggctgatt cggcactcag aagaattctg atcgattttt
     1801 actttcagat gatttgagcg ggaaatgcag ctgctgaaaa caaatatttc atatagcttc
     1861 cttttttctt gagagatgaa caggtatgca ctgtgacggc tttgaactgc ttatttcggc
     1921 tgaatggaca gataagagct ttttaattct ggtctgatcc ggttcattgc tttaagcagc
     1981 tttggattga agattccgct ctctccctga gtgatcatag tgaagacttt ttcttcatca
     2041 aatgcttctt tgtagcagcg tctgctgatc aatgcatcgt aaacatctgc gagcgatact
     2101 gtctgagcgg ccagggagat gttatcacct cgcagtccgt cagggcatcc agatccatca
     2161 tacctttcat gatggtggcg gcatatatca taacagtatt tataatattc ctgatcctga
     2221 attgcagcag cgctgttgat gatctcacat cctttaattg tatgtccttt cattatttca
     2281 aattcttcat cagtcagctt acccggtttc agaaggatac tgtccgggat tgctatcttt
     2341 cctatgtcat gcagtgcaga tgcagaagaa ataatgtcaa ttaattctag tgtgagttca
     2401 tactccggaa aatctaccat aatctgttca agaagaagcc gtgtcactgt cctgattctc
     2461 tgaatatgat ttcctgattc aaggttcctg gattcaacga ctgaggtaag tatgtcaatc
     2521 agctggttgt ttgcgatgcg agtttcattg tacttcctca gcatcacgag ctcattcata
     2581 tctatggatg tgatgacagt atccgtgctt attccgtcga ttcccttgaa tctgtatata
     2641 tcaagttttt tccagcgaaa tgagccgcct tttctcattc tgatgtctgc agttatgtag
     2701 ttttcggtgc tttttatgac ggtgtc
//
LOCUS       DAOQGJ010000337         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23181_length_2723_cov_2.750375, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000337 DAOQGJ010000000
VERSION     DAOQGJ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23181_length_2723_cov_2.750375"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PK683_06550"
     CDS             <1..902
                     /locus_tag="PK683_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08143.1"
                     /translation="IQKSMQERFSIQVEIDRSINRLIYRNGVFPVQGVRPVFSSIVDI
                     LESNLTKLIFEAMMESRNRISIAYSVEKKRIEAFVGEREHIIPYVGRIDKIRESNVRD
                     NVTSISVHESGHAVAYMVLTGLSPLQLKSRVANSYAGGFTFPHLVYETKETMTDKIRI
                     FLAGGIAEEIIFGEKHASVGRIQDRREATVLVLDYVRTYGFNSEYQSTYTLSEEYEMN
                     KYATDSFAERMMVKLVEETRVLLVEHIELLKSLSLVLSEKGSLESSEVAEIGLKFGVK
                     TEIREEGYLKIAGFGKILSGENE"
     gene            899..1666
                     /locus_tag="PK683_06555"
     CDS             899..1666
                     /locus_tag="PK683_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNH08144.1"
                     /translation="MKFSDAVHDLEEKLLKKIEPLHPILRNTVVTLVLFLLGLPGYLV
                     ANASINIDSAPSFGFWLDERIPFIPWTVAVYDWVYVIIFFPIFIVKDLDLIRHAAKAF
                     LFGIFVSIFFFTVLPAKIERPVIPKPANFIDWWVWINYTIDRPTTLFPSMHVSNAILT
                     AMTVLSFSKRAGYPILLLAVLISLSTLTMKQHFAADVIGGAVLAAVSYRIFLWPYVKE
                     TVSRRSEMLLPASYALIVPAGGLCATFVLYCIYRIVK"
     gene            2412..>2723
                     /locus_tag="PK683_06560"
     CDS             2412..>2723
                     /locus_tag="PK683_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH08145.1"
                     /translation="MENLVKIYNKRRVVDGASFNISKGEIVGLLGPNGAGKSTSFYMS
                     VGFTVPDEGSVFIDDIDVTNVPMFQRARLGVGYLAQEASIFRKLTVAENLEAVLETMN
                     LS"
BASE COUNT          756 a          510 c          624 g          833 t
ORIGIN      
        1 gaattcaaaa atcaatgcag gaaaggtttt cgattcaggt tgaaatagac agaagcatta
       61 accgtctcat ctacaggaac ggagttttcc ccgttcaggg agtaaggcct gtattctcaa
      121 gcattgtgga catactcgaa agcaatctga ccaagcttat atttgaggcg atgatggaat
      181 ccaggaatcg aatcagcatt gcatacagtg tcgaaaagaa gcggattgag gcgttcgtag
      241 gagaaagaga gcacattata ccgtatgtag gacgtataga taaaatcagg gaaagtaatg
      301 taagagacaa tgttacaagc atcagtgtgc acgaaagcgg acacgctgtc gcctatatgg
      361 ttctcacagg tctttctcct cttcagctga aaagcagagt ggcaaacagt tatgcaggag
      421 gtttcacttt tcctcatctg gtatacgaaa ccaaggaaac aatgactgac aagatcagaa
      481 ttttccttgc aggcggaatt gcagaagaaa tcatcttcgg tgaaaaacat gccagtgtcg
      541 gaaggattca ggacagaagg gaagcgaccg ttcttgttct ggactatgtg agaacatacg
      601 gttttaattc tgaatatcag agcacatata ctctcagcga ggagtacgaa atgaacaagt
      661 atgccacaga ttcatttgca gaaaggatga tggtgaaact ggtcgaggag acaagggttc
      721 ttcttgtcga acatatcgag cttctgaaat cgctctcgct tgttctttcc gagaaaggaa
      781 gtctggaatc ttcagaggtt gctgagatcg gtctgaagtt cggtgtaaag acagaaatca
      841 gggaggaagg ttatctgaaa attgcaggat ttggaaaaat cctctcagga gaaaatgaat
      901 gaaattttcg gatgccgttc atgatcttga agaaaaactt ctgaaaaaaa ttgaaccttt
      961 gcaccctatc ctgagaaata cagttgtcac tcttgtgctg tttctgcttg ggcttcccgg
     1021 ttatctggtc gcaaatgctt ctataaatat tgattctgca ccttcattcg ggttctggct
     1081 ggatgagaga attcccttca ttccctggac agttgctgtc tatgactggg tatatgtcat
     1141 catttttttt cctatattta tcgtgaagga tctggatctg atccgtcatg cggccaaggc
     1201 atttcttttc ggaatatttg tttccatttt cttttttaca gtgcttccgg caaaaataga
     1261 aagacctgtc ataccaaaac cggcgaattt tatcgactgg tgggtctgga tcaattatac
     1321 gatagaccgg ccgaccacac tttttccgag tatgcatgtc agcaatgcga ttctcactgc
     1381 aatgactgtt ctctcctttt caaagagagc cggttatccg attcttcttc tggcggttct
     1441 catcagtctg tccactctga caatgaaaca gcattttgct gcagatgtga tcggaggcgc
     1501 agtgctcgct gctgtttcct acagaatctt tctctggcct tatgtaaaag aaactgtttc
     1561 cagaagatcg gaaatgcttc ttcccgcttc atatgcattg attgttcctg caggaggact
     1621 ttgtgctact tttgttctgt attgcattta tcggattgtg aaataagact cctcctgccg
     1681 gttatcagtc gctgcataag aaagtcggca tatacagggc ctttgatttt gaaattgcat
     1741 cgaatgacct gagtttgaga gaatagaatt ttgataattt tccagtcgtc tcactgttca
     1801 gattgatttt tttctgactt cgtgcttctc tgtgcagaac agcagtatcg atttcctctg
     1861 aaaatgtttc caaagatcag ctaaggaggt taagaggcag caggccgatg cataatgctg
     1921 aaggtgcgcg ggcatctccg ttctggtttt tctgaaatcc gaattccttt ttcagttttt
     1981 cataaatgca gtcttatcgg ttctgaacat ttccttgaat cgctgtgaaa tcggaatggc
     2041 agaaaaaacc ggcagatgtc tttctttttt ttcatcttct tacttatcgt actttcctta
     2101 ttttaagtgg actttctgtt ctgccgatcc tgaaaggttt tccaattgtt ttccggattt
     2161 ctttccagga agaatggttt acataggatt tttgaatctt ggcgatctga aaagcattct
     2221 gttttgaaaa tatgcatttg gtgcataact ggagcagaga attgtttggc tggaattcta
     2281 taattatact aggcaaaaat catgccagtc tttaaaaaaa atggttgacg taaatatttt
     2341 tcaattaaga atgggtgatc taaggtgagt ttgcaaagaa gataatggaa atcaaacaga
     2401 aaaatttccg gatggagaac ctggttaaga tctacaataa aagaagagtt gtggacggtg
     2461 ccagcttcaa tatcagcaag ggagaaattg tcggtctgct tggaccgaac ggtgctggaa
     2521 aatctacatc attttatatg tctgtcgggt ttaccgttcc ggatgaagga tctgtattca
     2581 tcgatgatat tgatgttaca aacgtgccta tgtttcagag ggcaaggctc ggggtaggat
     2641 atctggctca ggaagcttct attttcagaa aacttacagt tgctgagaat ctggaggctg
     2701 ttctggaaac aatgaatctt tcc
//
LOCUS       DAOQGJ010000338         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23200_length_2722_cov_2.935883, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000338 DAOQGJ010000000
VERSION     DAOQGJ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23200_length_2722_cov_2.935883"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..>2722
                     /locus_tag="PK683_06565"
     CDS             406..>2722
                     /locus_tag="PK683_06565"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08146.1"
                     /translation="MSRDTEIIERLKTEYKVTDKINEYKDFEKEKEIYYQYSSDRRSV
                     LELRIQNTSYGEIIYTEKLENLVLQMEGLEVLELSLFFEKEIQFDARYSVLKNLKELS
                     LGPALTFTSNLLLVYKNMKSIFVSNADNWDLFFEFLKRMHQTSLENLDLSGKDISTLP
                     TTVSELRNLKHLNLSWCKELSSLPEWIGDLPLLEILDVSNTRISTLPASASKLKNLKS
                     LNFSDCEELNSLPDWIGELSALENLTLFKTKISMFPASVSELKNLKNLNLSGCDELNS
                     LPDWIGELSLLEILDVSNPRISTLPASASGLKNLKSLRLFGCNELDSLPEWIGELSLL
                     KTLVLSYTRISTLPVSVSKLKQLKELSLYGCKEINIIPEWLGDLSLLESLDISDTRIS
                     TLPSSVSKIKNLQYLSLSGCTELNSLPDWIGELSLLEILDVSNTRISKLPSSVSKLKN
                     LKDLNLYGCNELNSLPEWIGQLSSLEFLSLSSAKISNLPSSISKLKNLKILSLFWCKE
                     LTALPESIGELSLLETLGLTYTKINTLPSSFSQLKRLKNLTLSDCKELNSLPEDIGEL
                     SSLEDLVLSGTKISILPDSFSQLKNLKSLSLSEELTFLPETIGKLSSLKFLNLRNTKV
                     STLPSFLNHLKNIKRIDLLDSCISVLPEWITDFDLPILYDEEKRVFGGPGINLTGNPI
                     ETPPLEIVKQGNQAIKEYFRQIREQGVDVLYESKLIIVGEGGSGKTTLSKKLQNHSLL
                     LPETESTLGVEVNEGLQFTHSEDENIQIAVNV"
BASE COUNT          824 a          564 c          569 g          765 t
ORIGIN      
        1 cccgcttcgc aggaagcaga gctgatgcga aatgaaaaca gataagcgaa taaaccgcga
       61 agaacagaaa aagggaatag ggaataaccc gtcccttcgt gaacttcttg atgaagactt
      121 ttaattgtac tggtctgcct aaagaaatgc actcttcctc cgaaaacagt cttaactgcg
      181 aattgtagta actcctgcga acggctcatc tgtgtgatgc acacttcgct cgaaaacagc
      241 cttaactgcg aattgtagga actcctgcga acggcacacc tgagtgatac gcgcttcaca
      301 caaaaacagg ctcaactgtg aattgtagga actcctacag cctgacggga aagtccttgt
      361 cgtgtatagt ggacaggtaa caatggactg tcagcaggac aagagatgag cagagatact
      421 gagatcatag aaaggcttaa gacagaatac aaagtcacag ataaaatcaa tgagtataaa
      481 gatttcgaga aagaaaaaga aatttactat cagtattctt ccgaccggcg ttccgtttta
      541 gagttacgga ttcagaatac ttcatatggg gaaatcattt acacggaaaa actggaaaac
      601 cttgttctgc agatggaggg tctggaggtg ctggagttat ccttattttt tgaaaaggaa
      661 attcaattcg atgcaaggta ttctgtcttg aaaaatctga aggaactttc cttgggtcct
      721 gctttgactt tcacctcaaa tctgcttctg gtatacaaaa atatgaaaag cattttcgtt
      781 tcgaatgcag acaattggga tttgtttttt gaatttttga aaagaatgca tcagacatct
      841 ttggaaaact tggatctcag tggcaaagat atcagcacac ttccgaccac cgtttcagag
      901 ctgaggaatc tgaaacatct gaacctttcc tggtgcaaag aactgagctc cctgcctgaa
      961 tggataggag accttccact gctggaaatt ctggatgtta gcaatacaag aatcagtaca
     1021 ctgcctgcct ccgcttcaaa actgaagaat ctgaaaagcc tgaatttttc cgactgtgaa
     1081 gaactgaact ctttgccgga ttggataggg gaactttccg cgctggaaaa cctgactcta
     1141 ttcaaaacaa agatcagtat gttccctgcc tctgtttcag aactgaagaa tttgaaaaac
     1201 ctgaatcttt cgggatgcga tgaactgaat tccttgccgg attggatagg ggaactttcc
     1261 ctgctggaaa ttctggatgt tagcaatcca agaatcagta cactgcctgc ctctgcttca
     1321 gggcttaaga atctgaaaag cctgaggctt tttgggtgca atgaacttga ttcccttcct
     1381 gaatggatag gtgagctttc cttactgaaa actctggttc tcagttacac aagaatcagt
     1441 acgttgcctg tctctgtttc aaagctgaag caactgaaag aactgagcct ttatgggtgc
     1501 aaagaaataa acatcattcc tgaatggtta ggtgaccttt ccctgttgga atctctggat
     1561 atcagcgata caagaattag cacacttcct tcctctgtct ctaagataaa gaatctccaa
     1621 tatctgagcc tttccgggtg cacagaattg aattccttgc cggattggat aggggaactt
     1681 tccctgctgg aaattctgga tgttagcaat acaagaatca gcaaacttcc ttcctctgtt
     1741 tcaaagctga agaatctgaa agatctaaac ctttatgggt gcaatgaact gaattcactg
     1801 cctgaatgga ttggccaact ttccagtctg gaatttctga gtctctctag tgcaaagatt
     1861 tccaatcttc catcttccat atcaaaactg aagaatctga aaatactgag ccttttctgg
     1921 tgcaaagaat tgaccgcact gccggaatcg ataggtgaac tttcccttct tgaaactttg
     1981 ggtctcacct atacaaagat caacactctt ccatcctcct tctcacagtt gaaacgttta
     2041 aaaaatctga ccctctccga ttgtaaagaa ttgaactccc tgcctgaaga tataggtgaa
     2101 ctttcgtctc tggaagatct ggtacttagt gggacaaaga taagtattct tcctgactcc
     2161 ttctctcaat tgaagaacct gaaaagccta tccctttctg aagaactgac ctttttgccg
     2221 gaaaccattg gtaaactttc ttcactcaaa tttctgaatc ttaggaatac taaagtcagc
     2281 acgcttcctt cctttttgaa tcatcttaaa aatataaaac gaatagattt actggatagt
     2341 tgcatttctg tcctgcctga atggatcacc gattttgacc tgccaatctt atatgatgaa
     2401 gaaaaaaggg tttttggtgg tccaggtatc aatctcacag gcaatcccat tgaaacgcct
     2461 cctctggaaa ttgtaaaaca agggaatcag gcaatcaagg aatactttag gcagattcgg
     2521 gaacagggag tcgatgttct atatgaatct aagcttatca ttgtaggaga agggggttca
     2581 gggaagacaa cactgtctaa aaaacttcag aatcattctt tgttgctccc tgagactgaa
     2641 tctactttgg gagtagaagt gaatgaagga ttgcagttta ctcattcgga ggatgaaaat
     2701 attcagattg cagtcaatgt ct
//
LOCUS       DAOQGJ010000339         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23216_length_2721_cov_2.965866, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000339 DAOQGJ010000000
VERSION     DAOQGJ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23216_length_2721_cov_2.965866"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PK683_06570"
     CDS             complement(<1..427)
                     /locus_tag="PK683_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08147.1"
                     /translation="MKFYLFILFFMLGLSLSANETIELTENVEDANYKVVRLLNRLSD
                     NTTVPVEKTRGFNYRYATAWYTPYRYEVYVGKMGKKNETSLIRIEAPRRGEAKVLRSI
                     FEEEILQGESEKNEAAERIRSKSHILNQGFNMLAPSLGIL"
     gene            complement(441..1319)
                     /locus_tag="PK683_06575"
     CDS             complement(441..1319)
                     /locus_tag="PK683_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNH08148.1"
                     /translation="MVEDLPANNKDRFYISFLQIIITIFCLITSTVFMQFSMMSALYY
                     NRLPDDFKDSYSAMSSSEKEKVQKSLSENLAQEVKTEPDRLTQEYYRLVIGKYPFILV
                     INSIFWAISFLLPAYIFFGRYLKAPGAELGAELSFQTVSRGLLAGAFTFAVVSLFSLI
                     FYFFDYKPHVGDFQKILFKEMKGNVSLFAWSVYSVGMLTGIFEELYFRGYLLTHFVKS
                     GHARFGLFFTSILFGLMHYSFGVSAFVPFITAVVGYFFASFYLFTGNIWISASAHAAY
                     NSLGLLTAFLLGDKLL"
     gene            1533..1913
                     /locus_tag="PK683_06580"
     CDS             1533..1913
                     /locus_tag="PK683_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08149.1"
                     /translation="MIENMNTLEAYETLKKVQFTDEQSREIVRLIVRVHETEPASRKD
                     LMESESNVRMEMKNTELRLQKEIEDIRLEIKNTELKLSKEIKELDTKLSVKLEKIHSD
                     FIRWLIGAMIAAGGLLFTALRFTH"
     gene            complement(1934..2479)
                     /locus_tag="PK683_06585"
     CDS             complement(1934..2479)
                     /locus_tag="PK683_06585"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH08150.1"
                     /translation="MITASVQTLENRVPISVEAFHMLYNQGLIPEKAELIEGVIYSKM
                     PKNPIHSNVLRKLNLYLYKALGDQFILSSENPISFDDSEPEPDISVLPLGDYSESHPK
                     TAILVVEIANTSLSFDTRKASVYAKGAVPEYIIFNLADSKMEVYRSLKESVYTEIRIL
                     SKEEFISSVLPNVRFSLQDFL"
     gene            complement(2558..>2721)
                     /locus_tag="PK683_06590"
     CDS             complement(2558..>2721)
                     /locus_tag="PK683_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HNH08151.1"
                     /translation="MINGERVINEDEFQFLRRIEEAGADLQKIYEGISGFGKIIDDFS
                     LLKITYSHE"
BASE COUNT          885 a          560 c          539 g          737 t
ORIGIN      
        1 gcagaattcc gagagatgga gcgagcatat tgaaaccctg attcagaata tggcttttgg
       61 accggattct ttcggcagcc tcatttttct ccgattctcc ctgaaggatt tcttcctcaa
      121 atatgcttct cagaactttt gcttcccctc ttctgggagc ctctatccgg atcagacttg
      181 tttcattctt ctttcccatt ttccctacat acacttcata tctgtaaggt gtataccatg
      241 cagtcgcgta tctgtagttg aatcctcttg tcttttctac agggacagtc gtattgtcgg
      301 aaagcctgtt cagaagtctg actaccttgt agttcgcatc ttccacattc tcagtcagtt
      361 caattgtctc atttgcagac agactgagac ccagcataaa aaataatata aaaagataaa
      421 atttcatgat aagaacaccc ttaaagaagt ttgtctccga gaaggaatgc agtcagaaga
      481 cccagagaat tgtaagcagc atgagctgat gcagaaatcc agatatttcc tgtaaaaaga
      541 taaaacgaag caaaaaaata acctacaact gcagtaataa aaggcacaaa agcagaaaca
      601 ccgaaactgt aatgcatcag accgaaaagg atggatgtaa aaaaaagacc gaatcgcgca
      661 tgaccggatt tgacaaaatg agtcagaagg taccctctga aatacagttc ttcaaaaatc
      721 ccagtcagca ttccgactga atagaccgac caggcaaaaa gggaaacatt gcccttcatt
      781 tccttgaaaa ggattttctg aaaatctccc acatgaggct tgtagtcaaa gaaataaaaa
      841 atcagggaaa ataatgatac cactgcaaat gtaaatgcac ctgcaagaag acctctcgaa
      901 acggtctgaa aactcaactc tgctccgagt tctgcccccg gtgccttcag atatcttccg
      961 aaaaaaatgt atgccggcag aagaaaactg atcgcccaga agatgctgtt gattaccagg
     1021 atgaagggat attttccgat tacgagtctg taatattcct gagtcagtct gtcaggttct
     1081 gtcttgactt cctgtgcaag gttttcagaa agtgacttct gaactttttc tttttcagaa
     1141 ctgctcatcg cactgtacga gtccttgaaa tcatccggaa gacggttgta gtacagtgcc
     1201 gacatcattg aaaactgcat aaatacagta ctcgttatca gacagaaaat tgtaataatt
     1261 atctgaagaa aactgatata aaaacggtct ttattatttg caggcagatc ttctaccata
     1321 tattccacaa tgaaaaaaaa accgaaactt ctgtcaagat aaggaatgag agcgaattcg
     1381 cttcgtcggg atagctgata ggcgacaaag ggcggttcaa tgccggaatg acagtgtcgt
     1441 gcaacgatta cattctcccg tttttattcc actaaaagtg caagaaccag aagaaaaaat
     1501 tcaattcttg acattttttt acaggtgtat ttatgataga aaatatgaat accctcgaag
     1561 catacgagac actcaaaaaa gtacagttca cagatgaaca gtcaagggag atcgtaagac
     1621 tcattgtcag ggttcacgaa acagaaccag caagcagaaa ggatctgatg gaatcagaat
     1681 ccaatgtccg aatggaaatg aagaacacag aacttcggct ccagaaagaa atcgaagata
     1741 tccgtctgga gataaaaaac acagaattga aactgtctaa ggagatcaag gagctggaca
     1801 caaagctcag tgtgaaactc gaaaaaatcc attccgactt tattcgctgg ctcattggtg
     1861 caatgattgc tgcaggcgga cttcttttca ctgcgttgag gtttacgcat taagggtatg
     1921 cagtatccca gcgttaaaga aaatcctgga gagaaaatct cacattcgga aggacggagg
     1981 aaataaattc ttctttggaa agaattctga tctccgtgta aacggattcc ttcagcgatc
     2041 tgtacacttc cattttcgaa tccgcaaggt tgaaaatgat atattcagga acagctccct
     2101 ttgcatagac agaagctttt cttgtgtcaa aagaaagtga agtatttgca atttccacga
     2161 ccagaattgc tgtctttgga tgagattcgg aataatcacc caagggaagt actgatatat
     2221 ccggttccgg ttcagaatca tcaaatgaaa tggggttttc tgaactgagg ataaactgat
     2281 ctcctagagc tttatacaaa tacaggttca gttttctcag aacattggaa tgaattggat
     2341 ttttaggcat cttggaataa attactcctt caatcagttc tgccttttcc ggaatcagtc
     2401 cctgattgta cagcatatga aatgcctcaa cagaaatggg aaccctgttt tctaaagttt
     2461 gaactgatgc tgtaatcata actgaatatt ctgaattgaa ataaattcag tcaagcaaaa
     2521 agaagctgag gcgaaggatc agaatccctc cctgcagtca ttcgtgactg tacgtaattt
     2581 tcagaagaga gaaatcgtcg atgatttttc cgaaaccgga aatgccttca taaattttct
     2641 gaagatccgc acccgcttct tcaatccttc tcaggaactg aaactcgtct tcattgatga
     2701 ctctttcccc gttaatcatt t
//
LOCUS       DAOQGJ010000340         2719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23241_length_2719_cov_3.388889, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000340 DAOQGJ010000000
VERSION     DAOQGJ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23241_length_2719_cov_3.388889"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PK683_06595"
     CDS             complement(<1..140)
                     /locus_tag="PK683_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNH08152.1"
                     /translation="MLFQIALYRPEIPPNTGNIARLCVGLGVPLHIVGTPSFDLSEKA
                     VR"
     gene            complement(141..1268)
                     /locus_tag="PK683_06600"
     CDS             complement(141..1268)
                     /locus_tag="PK683_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNH08153.1"
                     /translation="MKNSFAELLEESFKKRKTITQGSSHEVLVLSVQDDYVFIKSRSG
                     LQGIIAKDEFLPEEVPSKGKQMNAFFLKEVHGDYYFTVCLQPDSLTKELLELARENEI
                     PILGQLGIESNGGYEIKLGEFSGFCPASQFDLEKKGKNLTGQKIKFVINDINEKTWKL
                     SLSQKRISDKEKALKVEILQSELKEGSFVSCTVKSIHNFGLIVDMNGLDALVPISEAS
                     FKKNPKLDQEFSVGQKLRAKVLNLDWSANKFSLSLKDSLEDPWSKKIPFKEGDIVKAE
                     VDSIKPFGLFIRFNENFHGLVPNKETGLAQRVPLANHFKPGDPLDVFILEVNPVKRQI
                     AASIVKAAEAKERLEYESYLNTQAVSNVSSFGLALKKSLKK"
     gene            complement(1278..1502)
                     /locus_tag="PK683_06605"
     CDS             complement(1278..1502)
                     /locus_tag="PK683_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08154.1"
                     /translation="MSQNLTEVLANTKLAKDLAQEFNIPVETAAKRILIALNNPDFTD
                     SIYESMKNYLNKIENLVVTDGRIEIVSMDN"
     gene            complement(1690..1908)
                     /locus_tag="PK683_06610"
     CDS             complement(1690..1908)
                     /locus_tag="PK683_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08155.1"
                     /translation="MAANMNEEEEWEIFQDQKKLAMSTIDDLTHLKIELIEANIPVPN
                     FINNAIRYLKQKYLVGAQTIGEMIRVKN"
     gene            complement(1919..>2719)
                     /locus_tag="PK683_06615"
     CDS             complement(1919..>2719)
                     /locus_tag="PK683_06615"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH08156.1"
                     /translation="FRERHMKNLFFTAVFFLLAGVPTFTFLKEYSDGQTLPAGRSYSG
                     IAIDRVVTTLKDVTKFKDLMLYLTSLFFAMASLGIVISFAFIYGKQEVHIDDKIHKPV
                     MFIVLQFTAAAGAYLFGVIQDRIGALKTFNLTVYFWIVSVIVILQVAPITGFLNSMLG
                     TNFSVQWMFVLVTSLAGLGLGATQSSSRAIVAIFAPESKVGEFFGLWGFSGKLAAAFG
                     LLLLALLQSFMSFRGSFAIIAVFFAVSVLINRFVDEERGAKAASDYKE"
BASE COUNT          770 a          645 c          513 g          791 t
ORIGIN      
        1 cttcgaacag ccttttcgga cagatcaaag gaaggagttc ctacaatatg gagaggaacc
       61 cccaatccta cacagagcct ggcaatgttt cctgtattcg gcgggatttc aggccgatac
      121 agagcaatct gaaaaagcaa ttactttttc agagatttct ttaaagccag gccgaaactg
      181 gatacattgc tgactgcctg agtattcaga tagctttcat attccagtct ttcttttgcc
      241 tctgcagctt ttacgatgga agcggctatc tgtcttttga ccggatttac ttcgagtatg
      301 aagacatcca gcggatcccc cggcttaaaa tgattggcaa gcggaactct ttgagcaaga
      361 cctgtctcct tatttggaac cagaccatga aagttttcgt tgaagcgaat aaagagaccg
      421 aacggtttga tggaatccac ttctgccttg acaatatccc cctctttaaa aggaatcttt
      481 ttcgaccaag gatcttccag tgaatcctta aggctcagtg aaaacttatt tgcagaccag
      541 tccagattca gcacttttgc cctgagtttc tggccgacag aaaactcctg atccagctta
      601 ggatttttct taaaacttgc ctcactgatc ggtacaagcg catccagacc gttcatatcc
      661 acaatcagac cgaaattatg gatagacttg acggtacagg aaacaaaact tccttctttc
      721 agttcagact gaaggatctc caccttgaga gctttttcct tgtcggaaat ccttttttga
      781 gagagggata atttccatgt tttttcatta atatcattga tgacaaattt gattttctgt
      841 cctgtcaggt ttttgccttt tttttccaga tcaaactgag atgccggaca gaatccgctg
      901 aactccccca gcttgatttc ataaccgcca tttgactcaa ttcccaattg gccgagtatt
      961 ggaatctcat tttctcttgc aagttccaaa agttccttag tcaatgaatc aggctgaagg
     1021 catacggtga aataataatc cccatgcact tctttgagaa agaatgcatt catctgtttt
     1081 ccctttgaag gaacttcttc aggcagaaat tcatcctttg cgatgattcc ctgaagacca
     1141 gaccgagact taatgaatac atagtcatcc tgaacgctca ggacgagaac ctcatgagag
     1201 gatccttgtg taatcgtttt ccttttctta aagctttctt ccagaagttc tgcaaaactg
     1261 tttttcattg ctgttcctca gttgtccata ctgacaattt caattctgcc gtctgtcacc
     1321 accagatttt caatcttgtt cagatagttt ttcattgatt catagattga atctgtaaaa
     1381 tccggattat tcaaagcgat gagtattcgc ttggctgctg tttctacagg gatattgaac
     1441 tcctgtgcca aatcctttgc taatttcgta tttgctaaca cttccgtaag attctgactc
     1501 ataaacatgc ctgccaatca tcccctgttc cgattatttt acttttcagg caactgctca
     1561 gcacgcggct ttgctgttac tgattagggc ctgccgacgg ccataaaagc cgtttttatc
     1621 ctgctgctag gcggctgccc cttatgttac tcattttcta aaaaaaagga gaccgatttg
     1681 aatacaactc taattcttta cccgaatcat ttctccaatc gtctgggcac cgacaagata
     1741 cttctgtttc agatatcgaa tcgcattgtt gataaaatta ggcactggaa tatttgcttc
     1801 aatcagttca attttcagat gagtcagatc atctattgta gacatcgcca gttttttctg
     1861 atcctgaaat atttcccact cttcttcctc attcatattc gctgccatag acttctcctc
     1921 attctttata atcggacgcg gcctttgctc ctctttcttc atctacaaac ctgttgatga
     1981 gtaccgaaac tgcaaagaaa actgcgatga tcgcaaagga tcctctgaaa ctcataaaag
     2041 actggagaag ggcaagcaga agcagaccga atgccgcagc cagtttaccg gaaaatcccc
     2101 agagaccgaa aaactctcct accttagact ccggtgcaaa aatagcgaca attgcccggc
     2161 tggaactttg ggttgcccca agcccaagac ctgcaagaga tgtaacaaga acaaacatcc
     2221 actgaacgga aaaatttgta cccagcatac tgttcaggaa tccggtaatc ggtgccacct
     2281 gaagaatcac tatgacggaa acaatccaga aatacactgt aagattaaat gttttcagag
     2341 cccctattct gtcctgaatc actccaaaaa gataggcacc ggcagctgca gtaaactgaa
     2401 gcacgatgaa catgacaggt ttatgaatct tatcatcaat atggacttcc tgctttccgt
     2461 agataaacgc aaaactgatg acaatcccaa gcgatgccat tgcaaaaaaa agtgaagtga
     2521 ggtaaagcat cagatccttg aacttagtca catccttcaa tgtagtaact actcggtcta
     2581 tcgcaattcc agaataggac cgtcctgcag gaagtgtctg cccatccgaa tactctttca
     2641 ggaaagtgaa tgtaggaact cccgcgagaa gaaagaagac tgcagtaaaa aataggttct
     2701 tcatatgccg ctccctgaa
//
LOCUS       DAOQGJ010000341         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23268_length_2717_cov_3.926371, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000341 DAOQGJ010000000
VERSION     DAOQGJ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23268_length_2717_cov_3.926371"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PK683_06620"
     CDS             <1..481
                     /locus_tag="PK683_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNH08157.1"
                     /translation="ESSGPHSNGFSLLRKLFLKEGKHLPDDAKQIEFLREDLLRPTRI
                     YVEPVLKVLQKEEIKGMVHITGGGFHENIPRILQDGTSAALDYSKIPLHPVFQKISKD
                     FQIEMRELFSTFNMGVGYIVITSKENAETVRKEFEMLGEGAHIIGEIVSGKKEVIIS"
     gene            537..983
                     /locus_tag="PK683_06625"
     CDS             537..983
                     /locus_tag="PK683_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNH08158.1"
                     /translation="MHPVIDLKKLYEEDEHLWLFENAKLLREGRFELADMEHIAETLE
                     DMGKRDFREILSRMRVLMVHLLKWIFQPKARSSSWKGTIVEQRKELKKMFKDSTNLKK
                     YAESNWLEEYSDARDIASAETGLSLSTFPEEPPFSFEEVTDEKYLP"
     gene            complement(1022..1342)
                     /locus_tag="PK683_06630"
     CDS             complement(1022..1342)
                     /locus_tag="PK683_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08159.1"
                     /translation="MDSVQGTWIRTSWRWTEKTGDLSEKGFVHSSFLCDIEDEKCSDL
                     QRFVDYFKDICKQYCYSRKSFEEGDAGIMWSKPAEFSLSESDGGYRLYLGTKYVPTLI
                     EYGP"
     gene            complement(1391..1993)
                     /locus_tag="PK683_06635"
     CDS             complement(1391..1993)
                     /locus_tag="PK683_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08160.1"
                     /translation="MMYFRKKDRDSIEAVIVGLDHSLEENNMEDKNDSVLRSLKLRSC
                     SACGQDGRQVTLLNYPVIQELHKGRNGFFGKIYDLYWQKWLTVSVKNASEKRMELRAY
                     AGFPISGKNIYLERADAELHKLIESAEKKKPSLPGNYYAVSLDFPFEKRTEVIKSLDE
                     ESFQIADVYGRLYPSNANLQEVRERSSSKVLHQMVFVKRE"
     gene            complement(2151..>2717)
                     /locus_tag="PK683_06640"
     CDS             complement(2151..>2717)
                     /locus_tag="PK683_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNH08161.1"
                     /translation="SVPDSANVAALGYAEESGIPYATGLIRSHYIGRTFIEPDQKIRD
                     FGAKIKYNVIREVVRDKRIIIVDDSIMRGTTSRKIIKMIRNAGAKEIHFRISAPPTVS
                     PCYYGIDIPTHSELIAATHTIDEIVKYLRVDSLAYLSTELMHTAVAKSMEESDYCDAC
                     FTGKYPVEFRKSDEVKEKYLFSELSEGE"
BASE COUNT          812 a          614 c          533 g          758 t
ORIGIN      
        1 cgaatccagc ggaccgcaca gcaacggatt ttctcttctc agaaaacttt ttttgaaaga
       61 aggaaaacat ctcccggatg atgcaaagca gatcgagttt ctccgtgaag accttcttcg
      121 gcctacaagg atttatgtcg agccagttct caaggtcctc cagaaggaag aaatcaaagg
      181 aatggtccat atcactgggg gcgggtttca cgaaaatatc cccagaattc ttcaggacgg
      241 aacctcagcc gcattagatt attctaaaat accccttcat cctgtatttc agaaaatctc
      301 aaaggacttt cagatagaaa tgagggaact cttttccacg ttcaatatgg gagtgggata
      361 cattgtaatt acatccaaag agaatgcaga aacagtcaga aaagaattcg aaatgctcgg
      421 cgaaggcgct cacatcatcg gagagatcgt ctcaggtaaa aaggaagtga ttatttcctg
      481 accgatgatc agagaaacag cttgacagaa aatcaagaga gttccagaat cggaatatgc
      541 atcccgtcat tgatctgaag aaactttacg aagaagatga gcatctctgg cttttcgaaa
      601 atgcaaaact tcttcgagaa ggcagatttg aactcgcaga tatggagcac attgcggaaa
      661 cactggaaga tatgggaaaa agagatttca gagaaatact gagcagaatg cgagtgctga
      721 tggttcatct gctcaaatgg atatttcagc cgaaagcacg ttcaagcagc tggaaaggca
      781 caatcgttga acagagaaaa gaactgaaaa aaatgttcaa agacagcaca aacttaaaga
      841 aatatgctga atccaattgg ctggaagaat acagtgatgc aagggatatt gcatctgcgg
      901 agacaggtct ctccctctct acttttccag aagagcctcc tttcagtttt gaagaagtga
      961 cagacgagaa atatttacct taagcgagag ttgaatcagt catcaacaaa tctgcatcaa
     1021 attagggacc atattcaatc agagtaggga cgtatttcgt accgagataa agcctgtaac
     1081 ctccatctga ttcggataat gaaaactcag caggttttga ccacataatc cctgcatccc
     1141 cttcttcaaa cgattttcgg gaataacaat attgtttgca tatatcctta aagtagtcta
     1201 cgaaccgctg cagatcactg catttttcat cctctatatc gcataaaaaa ctgctatgca
     1261 caaaaccttt ttcagaaagg tcacctgtct tttctgtcca cctccaggat gttcttatcc
     1321 aggttccttg aacagaatcc acactttcct tgcttttaga tatctccgaa agaattaacg
     1381 gtctcttccc ctactctctc ttcacaaaga ccatctggtg gagcaccttg gaagaagaac
     1441 gttctcgaac ttcctgaaga ttggcattgg aaggatagag tcttccgtac acatctgcta
     1501 tctgaaagct ttcttcatcc agtgatttaa tcacctcagt ccttttttca aatggaaaat
     1561 ccagagaaac tgcatagtaa tttcctggaa gagaaggctt ctttttttct gcactttcga
     1621 tcagtttatg cagttctgca tcagcccttt ccagatatat gttttttcct gagatgggaa
     1681 accctgcata ggccctgagt tccattcttt tttcagatgc attttttaca gaaacagtaa
     1741 gccatttctg ccagtacaga tcataaattt tcccaaagaa gccgtttctt cctttatgaa
     1801 gttcctgaat gaccggataa ttcagaaggg ttacctgtct tccatcctgc ccgcaggcag
     1861 aacagcttct aagtttcaga gagcgcaata cagaatcatt tttatcctcc atattgttct
     1921 cttcaagcga atggtccaga ccgacgatca ctgcttcgat actgtccctg tcttttttcc
     1981 gaaaatacat cataatggag tcccttgcga aattggactc accgctgacc ctggaaactt
     2041 ctttccaaag tccgaggatg tcgttttcac tgactgcagc attcgaaaca ctgcagccga
     2101 gaaaaaaaga aaatacaatg tatcccagaa ttgaacagaa aagtttcagt ttattctccc
     2161 tcggaaagtt cggaaaaaag atacttctct tttacttcat ccgactttct gaactctact
     2221 ggatatttcc cagtaaaaca tgcatcacag tagtcagact cttccatcga ctttgcaact
     2281 gcagtgtgca tcagttccgt ggacagatat gcaagcgaat ccactctgag atactttaca
     2341 atctcgtcta tcgtatgagt cgctgcaatc agttcactgt gcgtcggaat atcaattcca
     2401 tagtaacagg gagacacggt aggcggcgcg gatatcctga aatgaatttc cttcgcaccc
     2461 gcattacgga tcatttttat gatcttcctg cttgttgtcc ctctcatgat ggaatcatct
     2521 acaatgatga ttcttttatc tctcactact tctctgatga cattgtactt tatctttgca
     2581 ccgaagtctc tgatcttctg atccggctca atgaatgtcc ttccgatata gtgtgaacgg
     2641 ataagaccgg ttgcataggg aatgccggat tcttctgcat aaccgagagc agccacattt
     2701 gcagaatcag gaacaga
//
LOCUS       DAOQGJ010000342         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23271_length_2717_cov_3.579264, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000342 DAOQGJ010000000
VERSION     DAOQGJ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23271_length_2717_cov_3.579264"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..479
                     /locus_tag="PK683_06645"
     CDS             84..479
                     /locus_tag="PK683_06645"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH08162.1"
                     /translation="MTKPIIQKIKFSDVSSETLYSQYLNPKVHSEITDSTAVISSKEG
                     KNFSAYDGYITGKNIRLLPDKMIVQSWKASDWKENEESVLILEFENSGEDCFLQMTHI
                     GVPESELSGISRGWKDFYWSPWKKYLKEL"
     gene            484..771
                     /locus_tag="PK683_06650"
     CDS             484..771
                     /locus_tag="PK683_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HNH08163.1"
                     /translation="MNNIAKAKIEIEYCTQCNWLLRAAWMAQELLTTFKDEIGELSLI
                     PGTGGILEIRAEGETVFSRKENGRFPDMKELKQLIRDRIAPGRNLGHSDRN"
     gene            complement(773..1117)
                     /locus_tag="PK683_06655"
     CDS             complement(773..1117)
                     /locus_tag="PK683_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH08164.1"
                     /translation="MIIDWQRINSFADPDSPEDQEWLKGMIQDLITDNDEKMSHLEAL
                     IQDPKQEELRALLHQIKGVCANFGIVKMQSAAAEGEKSIKESKLEAGLNFAKELPALW
                     KEAKEELSKKYS"
     gene            1285..2064
                     /locus_tag="PK683_06660"
     CDS             1285..2064
                     /locus_tag="PK683_06660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH08165.1"
                     /translation="METDDLVSVIMPVYNSESFLSQSLDSVILQTYKKLELLPIDDFS
                     RDSSRDILKKYSEKDPRIKPIFKTENSGSADSRNFGIRQAEGRYIAFLDSDDLWDTEF
                     LEKQLNFMKKHNSAFSFSSYRIIGEDNREILKPFIVKKLKVSYYDNLFYNSVGQLTAV
                     YDSGALGKMYFDVSLKSVRDDYALWLDILKKTDFGFGNPEILASYRIRKGAVTANKKK
                     LILPHYRMLRNREKLSVIKAAFFTLVWGILGIRKYFLNRID"
     gene            2074..>2717
                     /locus_tag="PK683_06665"
     CDS             2074..>2717
                     /locus_tag="PK683_06665"
                     /inference="COORDINATES: protein motif:HMM:NF037162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08166.1"
                     /translation="MYSISASFQPGNPASELFLRTAGNVQFKDGKIQMEKDSSVFLST
                     YFSSFSAFKWAKYTSLRKIYLQLNCEGSCILRLFSENAEGKKLISEKEHSGTGRTDII
                     LEDTYSQPEIIYAELQSKDKVLFEGGFWGTDEIPEQSVNTAFIICTFRREEYVQRNLR
                     NIFDNLKQTETAPFSVYVSDNGKTLPETDSENLKIFHNSNSGGAGGFTRGIIEA"
BASE COUNT          887 a          530 c          555 g          745 t
ORIGIN      
        1 agagattgca gaaaagtttt tttcaaagga aacaatcaac agcagagttc tttccatata
       61 tctggacaat atgaggagca gatatgacaa aaccgatcat tcaaaaaatt aagttttcag
      121 atgtttcttc agagactctg tattcacagt atctgaatcc gaaagtgcat tctgaaatta
      181 cggattcaac tgctgtcatt tcttcaaagg aaggaaaaaa tttttccgca tatgacggat
      241 acataaccgg aaaaaacatt cgccttctcc cggacaaaat gattgttcag tcatggaaag
      301 catccgactg gaaggaaaat gaagaatctg ttctgattct cgaattcgaa aacagcggag
      361 aagactgttt tctgcagatg actcatattg gagtgcctga atcagaattg tccggaatca
      421 gcaggggatg gaaagacttt tactggagtc cctggaaaaa gtatctgaag gaattataat
      481 ataatgaata atattgcaaa agcaaaaatc gagatagaat actgcaccca gtgcaattgg
      541 cttcttcggg cagcctggat ggctcaggag cttctcacca cattcaaaga tgaaattggc
      601 gaactgtctc ttatccctgg aacaggcgga attctggaaa tcagagcaga aggagagact
      661 gtgttttcca gaaaggaaaa cggaagattt ccggatatga aggaattgaa acagctgatt
      721 agagacagaa tagccccggg cagaaacctg ggacacagtg acagaaatta attcaggagt
      781 attttttcga aagttcttct tttgcttctt tccagagagc cggaagttcc tttgcaaaat
      841 tcaaaccggc ttcaagtttg gattctttaa ttgatttttc accctcagca gccgctgact
      901 gcatttttac aattccaaaa tttgcacata cccctttgat ctggtgaagg agagctctta
      961 actcttcctg ctttgggtcc tggataagtg cctcaagatg actcattttc tcgtcgttgt
     1021 ccgtaattaa atcttgaatc atccccttca gccattcctg atcctcaggg ctgtccggat
     1081 ctgcaaaact gttaattcgc tgccaatcta taatcatcag atttcctttt atccactttt
     1141 ttttctgttt tatttttcac tagaattttt acaaaccgat tttcaaattt cggaaaaatg
     1201 actctcaaaa cccaaccatt ttatcattga agcaggtcat tgaatgaaaa aaatggaatt
     1261 actgaattta ggaaaaaccg aaaaatggaa acagacgatc ttgtatcagt catcatgcca
     1321 gtctacaatt ctgaatcgtt tttgtctcag agccttgaca gcgtaatact tcaaacctac
     1381 aaaaaacttg aacttctgcc catagatgat ttctccaggg acagcagcag agacattctg
     1441 aaaaagtact ctgaaaaaga tccgagaata aaaccgattt ttaaaacaga aaactcgggt
     1501 tctgctgatt ccaggaattt tggaatccga caggcagaag gacggtacat tgcattcctt
     1561 gactcggatg atctctggga tactgaattt cttgaaaaac agctgaattt tatgaaaaaa
     1621 cacaattcag cattttcatt ctcctcatac agaatcatag gagaagacaa cagagagata
     1681 ttaaagccat ttattgttaa aaagctgaaa gtaagctact atgacaatct gttttacaat
     1741 tcggtcggtc agctgactgc agtatatgat tcaggagccc tgggaaaaat gtattttgat
     1801 gtttcactga aaagtgtcag agatgattat gccctctggc tggacattct gaaaaagact
     1861 gactttggtt tcgggaatcc ggagattctg gcaagttaca gaatcagaaa gggtgctgtc
     1921 actgcgaaca agaagaaact gattctgccg cattacagaa tgctgagaaa ccgcgaaaaa
     1981 ctgagcgtaa tcaaggccgc atttttcacc cttgtatggg gaattctggg aatccgcaag
     2041 tattttctga accgtataga ctgaataatg atattgtatt ccatttccgc ttctttccag
     2101 ccgggaaatc cagcttcaga acttttcctg agaaccgcag gaaatgtaca gtttaaggat
     2161 ggaaaaattc agatggagaa agattcttct gttttcctga gcacctattt cagttcattt
     2221 tcagcattta aatgggcaaa atacacatca ttgagaaaaa tttaccttca gctgaactgt
     2281 gaaggcagct gcatcctgag acttttttca gaaaatgcgg aagggaaaaa gctcatttca
     2341 gagaaagaac attcgggaac gggcagaacc gacataattc tagaggatac atacagtcag
     2401 cctgaaatta tatatgcaga attacagtca aaggacaaag tgctttttga aggggggttc
     2461 tggggaacag acgaaatccc tgaacagtcc gtcaatactg cattcatcat ctgcacattc
     2521 agaagagaag aatatgttca gagaaatctc aggaatattt ttgacaatct gaaacagaca
     2581 gaaacagctc ccttttcagt ctatgtctct gacaacggaa aaaccctccc agaaacggac
     2641 agcgagaatc tgaaaatatt ccacaattca aacagcggag gagcaggcgg tttcacaaga
     2701 gggatcattg aggcaat
//
LOCUS       DAOQGJ010000343         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23358_length_2711_cov_3.507530, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000343 DAOQGJ010000000
VERSION     DAOQGJ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23358_length_2711_cov_3.507530"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PK683_06670"
     CDS             complement(<1..920)
                     /locus_tag="PK683_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNH08167.1"
                     /translation="MNKNTLKRKVITIILGGGKGTRLLPLTEKRSKPAVGFGGKYRLV
                     DIPISNALNSGFDQIYVLTQFNSYSLNRHISRTYGFQSVHKQGFVEIIAAEQTTSSVK
                     WFEGTADAVRKVMPHLEGFRPEYVMILSGDQLYNMDLNQFMEIHLAEEDTDMTIAANV
                     VPPELAHGLGIIRQDSTGRAVDFYEKPQNLHDVESFRLPDGRFLASMGIYFFKASVLH
                     ELLEDPTLTDFGKEIIPDALKTKKIKVFTYDGYWEDIGTIKSFFNANLMLTDNLPRFN
                     LYIEETPFYTRARSLPPTKVNKSNLSHVLLS"
     gene            complement(936..1604)
                     /gene="purQ"
                     /locus_tag="PK683_06675"
     CDS             complement(936..1604)
                     /gene="purQ"
                     /locus_tag="PK683_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNH08168.1"
                     /translation="MFRTGIIVFPGSNCDRDIAYVLETYYNHKTDYIWHKDSIQGKYD
                     LIVLPGGFSYGDYLRSGAIARFSNAMKSLKEHIDGGGKVLGICNGFQILTEAKLLPGT
                     LMRNANLQHICKDVRLKIGSGSNPLTRGIPEERELTIPISHGDGCFFADADTLKEIED
                     KRQVFYRYSGENPNGSLNGIAGVCSDNFQIAGLMPHPERAIDPWAESLDGKIFFDSLF
                     NFFN"
     gene            complement(1604..1852)
                     /gene="purS"
                     /locus_tag="PK683_06680"
     CDS             complement(1604..1852)
                     /gene="purS"
                     /locus_tag="PK683_06680"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNH08169.1"
                     /translation="MFIGKVSITLKKSVLDPQGNAVMKALHDMGENTVADVRIGKYAE
                     VRLSSDSKEKAEEELHRICRSLLVNEVIESYRVELEKI"
     gene            complement(1834..2685)
                     /locus_tag="PK683_06685"
     CDS             complement(1834..2685)
                     /locus_tag="PK683_06685"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004491601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNH08170.1"
                     /translation="MMKPFYKGKVRDVYDLGENFLLSSSDRVSAFDCVFTEAIPEKGK
                     VLNGISNLWFRYFKDIPNHIQETDWNKFPSEFRTEEFKDRSVIVKKCRRIDFECVVRG
                     YLTGSGYKEYKERGTLADLLLPSGIQESEKLSEPAFTPAVKNDTGHDENISEKKMESM
                     VGKEIFSYLKSVSVRIYSEAAEKMLSAGIILCDTKFEFGFDGEKILLIDELLTPDSSR
                     YWALETYKKGTSPPSYDKQILRNYLESTGWNKEPPPPALPADIIQTLHLKYKELQRKL
                     ELCLSEK"
BASE COUNT          714 a          625 c          511 g          861 t
ORIGIN      
        1 gaaagaagca catgagaaag attagactta ttcacctttg taggaggaag cgatcttgct
       61 cttgtataaa aaggagtttc ctcgatgtaa aggttgaatc tggggagatt gtccgtgagc
      121 attaaatttg cattaaagaa tgatttaatg gttccgatgt cttcccagta tccgtcataa
      181 gtaaatacct tgatcttttt agtcttaagc gcatccggta tgatttcctt tccaaaatca
      241 gtcagagtag gatcttccaa aagttcatgc agaacagatg ccttgaagaa ataaataccc
      301 attgacgcaa gaaatcttcc gtcaggaagt ctgaacgact ccacatcatg cagattctga
      361 ggtttctcgt aaaagtctac tgcccttcct gtgctgtcct gtctgatgat tccaagacca
      421 tgtgcaagtt cgggaggaac cacattcgca gcaatcgtca tatctgtatc ttcttctgcc
      481 agatggattt ccatgaactg attcagatcc atattgtaca gctgatctcc ggacaatatc
      541 atcacatact caggacggaa tccttccagg tgaggcataa ctttccttac cgcatctgca
      601 gttccttcaa accatttcac acttgaggta gtctgctctg ccgcaatgat ttccacgaat
      661 ccctgtttgt ggacagactg aaatccgtat gttcttgaga tatgtctgtt cagagaatac
      721 gaattgaact gtgtcagtac atagatctgg tcaaaccctg aattcagcgc atttgaaatt
      781 ggaatatcca ccagtctgta ttttccgcca aaacccacag ccggttttga tcttttttct
      841 gtaagaggca aaagtcttgt cccttttccg cctccaagaa tgatagtaat cacttttctt
      901 ttcagtgtat ttttattcat ggcccccgct ccttcctaat taaaaaaatt aaacaatgaa
      961 tcaaaaaata tctttccgtc gagggactct gcccaaggat ctatggctct ttcaggatga
     1021 ggcatcagcc ctgctatctg aaagttatcc gagcatactc ccgcaatacc gtttaatgat
     1081 ccgttcgggt tctcccccga ataacggtag aacacctgtc ttttatcttc tatttccttg
     1141 agtgtgtctg catccgcaaa gaaacatccg tctccgtgag aaataggaat cgtcagctct
     1201 ctctcttccg gtatccccct tgtcaaagga ttgctgcctg agccgatttt aagtctgaca
     1261 tccttgcata tatgctgaag gtttgcattt ctcatcagag ttcctggaag aagtttggcc
     1321 tccgtgagaa tctgaaatcc gttgcagatt ccaagaactt ttccccctcc gtcaatatgc
     1381 tctttcagtg acttcattgc attggaaaat ctggcaattg ctcccgatct gaggtagtct
     1441 ccgtaggaaa atcctccggg aagcacgatc agatcatatt ttccctgaat agaatcttta
     1501 tgccatatat aatctgtctt atgattataa tacgtttcta ggacatacgc aatgtccctg
     1561 tcacagttgg agcctggaaa aacgataatt cctgtcctga acatcagatc ttctccagtt
     1621 caaccctgta ggattcaatc acttcattta ccagaaggct tctgcatatc ctgtgaagtt
     1681 cctcttctgc cttttccttt gaatctgagc tcagtcttac ctcagcatac ttgccgatcc
     1741 gcacatccgc aactgtattt tcacccatat catgcagggc tttcataacc gcattgccct
     1801 gagggtccaa gactgatttt tttaaagtaa tgcttacttt tccgataaac atagttcaag
     1861 cttcctttgc aattctttat atttaagatg taatgtctgt ataatatctg caggcagtgc
     1921 agggggaggg ggttctttat tccagcctgt cgattccaga tagtttctga gaatctgttt
     1981 gtcataactg ggaggagaag ttcccttttt atatgtttca agtgcccagt atctcgatga
     2041 atccggggta agaagttcat ctataagaag aattttttcg ccgtcaaatc caaattcaaa
     2101 tttggtatca cataaaatga ttccggcaga aagcattttc tctgcggctt ctgaataaat
     2161 ccggacggaa acagatttca gataagaaaa gatttccttt ccgaccatcg actccatttt
     2221 cttttccgag atgttttcat catgtcctgt atcatttttt actgcaggag tgaaagcggg
     2281 ttcagaaagt ttttctgatt cctgaatgcc tgaaggaaga agaagatctg cgagtgttcc
     2341 cctctcttta tattccttgt accctgaacc agtcagatat ccccttacta cacattcgaa
     2401 atctatccgt ctgcattttt ttacaatgac ggatctgtct ttgaactctt ctgttctgaa
     2461 ttcggaagga aatttattcc agtctgtctc ctgaatatga ttgggaatat ccttgaagta
     2521 tctgaaccag agattggata tcccgttcag cacctttcct ttttccggaa tagcttccgt
     2581 aaatacacag tcaaatgcag agaccctgtc ggaggaactt aaaaggaaat tctctcccag
     2641 gtcataaaca tcccttacct tgcctttgta gaatggcttc atcagaattc cagtttggtc
     2701 cagttctttt c
//
LOCUS       DAOQGJ010000344         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23394_length_2708_cov_2.937429, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000344 DAOQGJ010000000
VERSION     DAOQGJ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23394_length_2708_cov_2.937429"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /gene="ftsZ"
                     /locus_tag="PK683_06690"
     CDS             <1..926
                     /gene="ftsZ"
                     /locus_tag="PK683_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNH08171.1"
                     /translation="QEDKDKITQALKGADMVFITAGMGGGTGTGAAPVVAEIAKELKC
                     LVVGVVTMPFVTEGKRRAEIARSGLNNLRQHVDTLITIKNDSLLKVVDKKAPVQFAFK
                     VVDDVLLNAVKGISDIINYAGIINVDFADVRSVMKDTGDAIMGVGEGVGDNRVADAVD
                     QAVNNVLLEDSSIEGASSLLVNVSGGKDLSISDWSEVSQIITGQVDSNANIIIGLNED
                     EVLQDKIRVTVIATGFKRMPHIVSPIKKDKEKEQAALNQAKEERPAPKVVNILDTELL
                     ESTRSMKPKINSGYNFGEDYDIPTYLRRRAD"
     gene            922..1908
                     /locus_tag="PK683_06695"
     CDS             922..1908
                     /locus_tag="PK683_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNH08172.1"
                     /translation="MKSVLFVSTYPFGRENSLPREILKKTPLKVIYNPLNRKLKPSET
                     AELAKDADIIIAGTENLEPLIRSSERLKFISRVGIGLDSVPLRLCKEKGIRVSYTPDA
                     VTPAVTELTLGLILDLFRDISYCDREIRNGKWSRPVGRRIEHSVIGMLGFGRVGKGVA
                     RLLSSFRPKEVLVNDIKDIQKDILSFREKFGLNIRFAEKEEIYSVSDLVTVHMPLTSE
                     TRGLVNRDVLHLFRKDAFLLNTARGEIVHENDLYDSLKNREIQGAALDVFSEEPYKGR
                     LRELENIILTEHIGSCSVDCRIAMERESAEEALNFFEGKKLLREVENYDGEF"
     gene            complement(1926..>2708)
                     /locus_tag="PK683_06700"
     CDS             complement(1926..>2708)
                     /locus_tag="PK683_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08173.1"
                     /translation="LLGNAVKFTEHGEIEIRIQKMLNYNYNSPEQCFVRFSVRDTGIG
                     INPENSKRIFEAFSQADASTIRKYGGTGLGLTISNRLLKMMRSELELESEPGKGSIFS
                     FILNVKFENSISEETFHLNGIRKILIVDDNRANRLIIRDSLLFKNIGYEEADSGTEAL
                     NLLEKNSYDIVIMDYHMTEMNGLETVRRLRNSSSVSDRNVPIILLHSSSDDDTVSRHC
                     RELQIPLRIVKPVKMKDLFSVLEKMQMPDPENNQTHAGDGQK"
BASE COUNT          731 a          542 c          654 g          781 t
ORIGIN      
        1 cacaggaaga caaggacaag attactcagg cactgaaagg tgctgatatg gttttcatca
       61 ctgcgggtat ggggggcgga accggaacgg gggctgctcc tgtggttgca gagattgcaa
      121 aggaactcaa atgccttgtc gtgggcgttg tgactatgcc ttttgtgact gagggtaaac
      181 gtcgtgcaga aattgcaagg tcaggactca ataatctcag acagcatgtg gacacactga
      241 ttacaatcaa gaatgattcg cttctgaagg ttgtagacaa gaaggctcct gttcagtttg
      301 ccttcaaggt cgtggatgac gttcttctga atgcagtcaa gggaatcagc gatatcatca
      361 attatgcagg gattatcaat gtggactttg cggatgtgcg ttccgtgatg aaggacactg
      421 gcgatgccat tatgggagtc ggcgaaggcg tgggcgacaa cagagttgca gatgctgtgg
      481 atcaggctgt gaacaatgtt ctgctcgaag acagttccat tgaaggtgca agttctcttc
      541 tcgtcaatgt atcaggagga aaggatctct ctatcagcga ctggagcgag gtttctcaga
      601 tcattacagg acaggtggat tctaatgcaa atatcattat cggtctgaat gaggacgagg
      661 ttcttcagga caagatccgt gtgacagtga tagcgacagg attcaagaga atgcctcata
      721 ttgtttctcc gatcaagaag gacaaggaga aggaacaggc cgcactgaat caggcgaaag
      781 aagaaagacc tgctcctaag gtagtgaata ttctggatac agagctactc gaatctacaa
      841 gatctatgaa accgaagatc aattccggat acaatttcgg agaggattat gatattccta
      901 cttacctgag aaggagagca gattaaatcc gttctttttg tctccaccta tcctttcggc
      961 agggaaaact ctctgccgag agagatttta aagaaaacac ctcttaaagt catttataat
     1021 cctttaaacc gaaaactgaa accctctgaa actgccgaac ttgcaaaaga tgcagatatc
     1081 attatagcag gtacggagaa tctggaacct ctgatccgtt cctctgaacg gctgaagttt
     1141 atttcacgtg tagggatcgg tctcgattct gttccgctcc gtctatgcaa agaaaaagga
     1201 atccgcgttt cttatactcc cgatgcagtg acacctgcag tgactgaact gactctgggt
     1261 ctgatccttg acctttttcg ggatatatcc tattgcgacc gtgagattcg gaacgggaaa
     1321 tggtcaaggc ctgtcggcag gaggattgaa cattctgtga tagggatgct cggtttcgga
     1381 agagtcggaa aaggggttgc gaggcttctc tcttcattcc gtcccaagga agtccttgtg
     1441 aacgatataa aagatataca aaaggatatt ttatcatttc gtgagaaatt cggtctgaat
     1501 atccgatttg ccgaaaagga agagatctat tctgtttctg atcttgtcac tgtccatatg
     1561 cctcttacat ccgagacaag ggggcttgtg aacagggatg tacttcatct gttccgcaag
     1621 gatgccttcc ttttgaacac tgccagaggg gagatcgtac atgaaaatga tctgtatgat
     1681 tctctgaaga acagggagat tcagggagct gcactggatg tattttcgga agagccttac
     1741 aaaggcagac tgcgagaact ggagaatatt attcttacgg agcatatcgg ttcctgttcc
     1801 gtggactgca gaattgcaat ggaaagagag tctgccgaag aagcgctgaa ttttttcgag
     1861 ggaaaaaaac ttcttcgtga agtggaaaac tatgacgggg agttttgagt tttctgatcc
     1921 tgatcttatt tctgaccgtc tccagcgtgt gtctgattgt tttccggatc aggcatctgc
     1981 attttttcta gaacagaaaa aagatctttc atttttacag gtttgacaat tctcagagga
     2041 atctgaagtt cccggcaatg cctgctcact gtatcatcat ctgaggagct gtgaagaaga
     2101 atgatcggaa cgtttctgtc cgaaactgaa gaggaatttc tcagcctgcg cactgtttcc
     2161 agtccgttca tttcagtcat atgatagtcc ataattacaa tatcatagct gttcttttcc
     2221 agaagattca atgcttctgt tccggaatct gcctcttcgt atccaatgtt cttgaaaagc
     2281 aggctgtctc tgataatgag tctgtttgct ctattgtcat caacaatcag tatttttctg
     2341 attccgttca gatgaaaggt ttcttcggat attgaatttt caaatttgac attcagaata
     2401 aatgaaaata tgcttccttt tcccggttca ctttcgagct cgagttcaga tctcatcatc
     2461 ttcagaagtc tgttcgaaat tgtcagtcct aagcctgtgc ctccgtattt tcgtatggtc
     2521 gatgcatctg cctgtgaaaa tgcttcaaag attcttttgc tgttttcagg attgattcct
     2581 attccagtgt cccgcacaga aaatcggacg aagcactgtt ccggtgaatt ataattataa
     2641 ttaagcattt tttgtatacg tatctcgatt tccccgtgtt cggtgaattt gaccgcattg
     2701 ccgagaag
//
LOCUS       DAOQGJ010000345         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23504_length_2700_cov_2.424575, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000345 DAOQGJ010000000
VERSION     DAOQGJ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23504_length_2700_cov_2.424575"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK683_06705"
     CDS             <1..216
                     /locus_tag="PK683_06705"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNH08174.1"
                     /translation="GASSYQNLILESVNGITVRNIKHLKEICEGVQSGFLKFKFKDMP
                     SSFIIKKNDAMKADSEISEIYKIGKIK"
     gene            213..1685
                     /locus_tag="PK683_06710"
     CDS             213..1685
                     /locus_tag="PK683_06710"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNH08175.1"
                     /translation="MRAILAFLFLFHHLSAVSHTEDEMKKSIVQIKISVQSPNFMYPW
                     QLKKPSSFEAVGVAVEKGKILTLAVNLDYATSIEIKKHSSYVTEKAVVERIDYESNLA
                     VLSVSNESFFSDLIPVKFENRPGTDISGSIVQLDNSGTIQTAVGRITAIDSEMYPLGQ
                     TEFPFLSINSNEKLEGNGELILHKKKIQGILYKFTPGKSTGKAVPGFLINKFLSSKTK
                     GKSSVFPHKGFKFKGITDNSTKEYFGLDKNREGVIVSEIIPYSSADGVLETEDVITEF
                     GGRKIDSQGFFQHPEYGKVQLSFLAHSGDEFGFKKGRKLPVKILRDRKEMTVNLVLKS
                     FPYKSIRIPYLHNFGKHPSYLIRGGFVFTELSELLLREWGQNWRSRVDKKLLYYMDYK
                     KHHETRESHGKVVILVQVLPDESNNGYHSLSMDIVTLAGDMKVSSIKELDTVISRTET
                     PYIPIQLDNGVTIVLDKAGLKQTDENISKKFHIPVLSSIQ"
     gene            1795..>2700
                     /locus_tag="PK683_06715"
     CDS             1795..>2700
                     /locus_tag="PK683_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNH08176.1"
                     /translation="MTGLKTETAVSENWKNWKWQMQNRIRTKEELREHIELSSEEISA
                     FDLCRERFDFGVSPYYLSIADRTDLNCPIRRQIVPSLSELRKTENETEDPLGEEAHMP
                     VKGVTHRYPDRALWYLSHSCAVYCRFCTRKRKVGQTDSAPGRDEWEEALRYFRNRKEI
                     REVILSGGDPLSRSDSQIEYILEKLRDIPHINHIRIHSRYPVTLPFRITDELCTVLRK
                     FSPVFFVTHFNHAKELTEDARSACIKLQTEGNVVLLNQSVLLSGINDSAEALSDLFYG
                     LISFGVKPYYLHQCDDVFGSSGFKVP"
BASE COUNT          847 a          554 c          592 g          707 t
ORIGIN      
        1 ggagcatcct cttatcagaa tcttatactg gaatcagtaa acggaatcac tgtccggaac
       61 ataaaacatc tgaaggaaat ctgcgaggga gttcagtccg gcttcctgaa gttcaaattc
      121 aaggatatgc ctagttcttt tatcattaaa aaaaatgatg caatgaaggc agattctgaa
      181 atatcggaaa tttataaaat aggaaaaata aaatgagagc aattctagca tttctatttc
      241 tttttcacca tctgtccgct gtgtctcata ctgaagatga gatgaagaaa agcatagttc
      301 agatcaaaat ttctgtccag tccccgaatt ttatgtatcc ctggcagctg aaaaaacctt
      361 cctcgtttga agcagtcggt gttgctgtgg aaaaaggaaa aatactcacc ctcgcagtca
      421 atctggatta tgcgacttcc attgaaatca aaaagcattc ttcctacgtg accgaaaaag
      481 cggttgtaga aaggatagat tatgaatcca accttgccgt tctgagtgta tccaatgaga
      541 gcttcttctc cgacctgatt cctgtaaaat tcgaaaacag gcccggaaca gacatcagcg
      601 gatctatcgt gcagctcgac aattcaggaa caatacagac agcagtggga agaatcactg
      661 caatcgactc ggaaatgtat cctctgggcc agaccgaatt ccccttcctg agcataaatt
      721 caaatgaaaa actggaagga aacggagaat taattcttca taagaaaaaa atacagggta
      781 tcctgtataa gttcactccc ggcaaaagta cagggaaggc tgttccggga tttctgatca
      841 ataaattcct ttcttcgaaa accaagggaa agagttccgt cttccctcac aaaggcttca
      901 agttcaaagg aatcacggac aactccacaa aagagtattt cggtctggat aaaaaccgcg
      961 aaggggtaat cgtttctgaa atcattccgt attccagtgc agatggagtc ctggaaaccg
     1021 aggatgtaat cactgaattc ggtggaagga aaatagattc acagggattc tttcagcacc
     1081 ctgagtatgg aaaggttcag ctttcctttc ttgcccattc cggagatgag ttcgggttta
     1141 aaaagggaag aaaacttcct gtaaaaatac tcagagacag gaaggaaatg acagtgaatc
     1201 ttgtgctgaa gtcttttcca tataaatcca tacggattcc ctatctgcat aatttcggaa
     1261 aacacccttc ttatctcatt cgaggaggat ttgtattcac tgagctttcg gaacttcttc
     1321 tgagagaatg gggacagaac tggagatcca gagtggataa aaaacttctg tactatatgg
     1381 actataaaaa acaccatgaa accagagaga gtcacggcaa ggtcgtcatt ttggttcagg
     1441 tgcttcctga cgagtccaac aacggatatc acagtctgag catggatatt gtcacgttgg
     1501 caggggatat gaaagtaagc agtattaagg aactggatac agtcatttcc agaacggaaa
     1561 ctccttatat acctatccag ctggacaacg gcgtaacgat tgttctcgac aaggctgggc
     1621 tgaaacagac ggatgaaaac atttccaaaa agtttcatat cccggtactt tcaagtattc
     1681 agtgaaaatg aattcaacgg ggattatatt gaaagcttat tctggaacat tttatttttt
     1741 tgacttcaga attctgcatc agactggact gcagaaaact tgtctggaca acttatgact
     1801 ggactgaaga ctgagactgc agtctccgaa aactggaaaa actggaaatg gcagatgcag
     1861 aacagaatca ggaccaagga agaactgaga gaacatatag aattgtcttc cgaagaaatc
     1921 tctgcctttg atctctgccg tgaaagattt gatttcggtg tctcccccta ctatctctca
     1981 atcgcagaca ggacggatct gaactgtccg atcagaaggc agattgtgcc gagtctttct
     2041 gaacttcgga aaacagaaaa tgaaacggag gatcctcttg gagaagaggc gcatatgcct
     2101 gtcaagggag tgactcacag atatcctgac agagcgttat ggtatctttc acattcctgt
     2161 gctgtgtact gcagattctg tacaagaaaa agaaaagtgg gacagacgga ttccgcaccg
     2221 ggccgtgatg aatgggaaga agctctccgg tatttcagaa acagaaagga aatcagagaa
     2281 gtcattcttt ccggcggaga tcccctttcg agatccgatt cacagattga atatatactg
     2341 gaaaaactga gggatattcc ccatataaat catattcgca tccattccag atatcctgtc
     2401 acgcttccgt tcagaattac tgacgagctc tgcaccgttt tgagaaaatt cagtcctgtt
     2461 tttttcgtga ctcatttcaa tcatgcgaaa gaactgactg aggatgcaag gtcagcctgc
     2521 ataaaacttc agacagaagg aaacgttgta cttctcaatc agagtgttct tctctccgga
     2581 atcaatgaca gtgcagaagc tctttcggat cttttttacg gtctgatttc tttcggtgta
     2641 aaaccgtact acctgcatca gtgtgacgat gtattcggca gcagcggatt caaggttccg
//
LOCUS       DAOQGJ010000346         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23543_length_2697_cov_4.498107, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000346 DAOQGJ010000000
VERSION     DAOQGJ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23543_length_2697_cov_4.498107"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PK683_06720"
     CDS             <1..1128
                     /locus_tag="PK683_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08177.1"
                     /translation="EQESRSKSVFLANLSHEIRTPLNAILGFSEVLQNEIEDEKHQEY
                     LDSITRSGRNLLSLINEIFDLSKIEAGRLDIKNEKVNIHGVIQNMNRIFAHQLKDKGL
                     EFRTDVSGSFPETILFDENKLNQILLNLVGNAIKFTNIGFVKCSADVLKRNGEYIDFA
                     IQIMDTGIGMSQEMQNQVFSESSFHSDSLEYGRAGLGLSICIKLTKLLGGNIRLVSTQ
                     GTGSTFILEFRNVRILSSDFAKSGQSDYTGIRIIRSEILVVDDTALNIELIKQFLKKN
                     DVKVHSASSGEEAISLLESIHPHLIIMDLKMSGLDGFETTKIIKGNPATQNIPVLCYT
                     ASMMNTGSLDKYKIFNDFLGKPLIRADLLRALCRFLPFEKV"
     gene            complement(1133..2380)
                     /locus_tag="PK683_06725"
     CDS             complement(1133..2380)
                     /locus_tag="PK683_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08178.1"
                     /translation="MKFFQFFIIFAFLSVSVLAQQKPLRAADDRKYSYTVVEPVGFDS
                     SRKYILVVCLHGLRGNSAEMAGVLSNYTRYMNMVLLCIDGNIPDPERNARKWGYSESE
                     SYILETVHRIRSKYNLMPETLLIGYSQGASQALLTAVRNPDLFPFSANVSGGNITPDE
                     KNSENVSKLKVLFLAGDTGDAEKKIKLYLSEKSKLFSKKTDSTFKSFKNHKHDFSHHM
                     AYASLKWYMEKSRRYGSDFWMNKGDYLSYYEKGESLFEEGQYEKSRKYFRKSISINPF
                     FPPAALSYTHAALMLGNMSSFTKSFFPSLELYSSFPFFEEKRVFTLFNQLNATVSQDE
                     NLQKFLIRFLMDKIEQYAEVLHPAFAGEVYLTVFRLAIRNSMLEEAKTSRKKAMKIYS
                     KMDKNSAIYNDADIGNKMAELDK"
     gene            complement(2419..>2697)
                     /locus_tag="PK683_06730"
     CDS             complement(2419..>2697)
                     /locus_tag="PK683_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphodiesterase/nucleotide
                     pyrophosphatase"
                     /protein_id="HNH08179.1"
                     /translation="ADHGCAPLPEISGGQRLTVNKVFSALNSLLPPEEKESLAAFMTV
                     GQISLNRELVKKYGITESQIRKKILGIKGSKGTGFFEDVVFRSEIPVQ"
BASE COUNT          775 a          568 c          568 g          786 t
ORIGIN      
        1 gagcaggaaa gcagatccaa gtcggtgttc cttgcaaatc tcagtcatga gatcagaact
       61 ccgttaaatg caattttagg attcagcgag gttcttcaaa atgaaattga agatgaaaaa
      121 catcaggagt atcttgattc aatcactcga agcgggagaa atcttctttc tctcatcaat
      181 gaaatatttg atctttctaa aatagaagcc ggccgtctgg acataaaaaa tgaaaaagtg
      241 aacattcacg gagtcattca gaatatgaac agaatctttg cacatcagct gaaagacaag
      301 gggcttgagt tcagaacgga cgtcagcggc agctttcctg agactatact gtttgacgag
      361 aataagctga accagatact cctgaacctt gtcggaaatg cgattaaatt tacaaatatc
      421 ggatttgtaa aatgcagtgc tgatgtcctg aaaagaaacg gggaatacat tgattttgcc
      481 attcagatca tggatacggg tataggtatg tctcaggaaa tgcagaatca ggtgttttcc
      541 gaatcatcat ttcattccga ttcactggaa tatggaagag caggacttgg gctgtccata
      601 tgcatcaagc tgacgaaact tctcggaggc aatatccgcc ttgtcagtac gcagggaacc
      661 ggaagtactt ttatcctgga attcagaaat gtcagaatac tgagttcgga ctttgcaaag
      721 tcaggtcagt ccgattatac tggaatcaga atcatcagat ccgaaattct tgtagtggat
      781 gacactgctc tgaatattga actgattaaa cagttcctga aaaaaaatga tgtgaaggtt
      841 cattccgctt cgtctgggga ggaagcaatc tcccttcttg aatcgataca cccgcatctg
      901 atcattatgg atctgaagat gtcaggtctg gacggatttg aaaccactaa gatcatcaag
      961 ggaaaccctg ccactcagaa tatcccagtc ctctgctata cagcatcaat gatgaacaca
     1021 ggcagtcttg acaagtataa aatattcaat gattttctgg gaaaacctct gataagagcg
     1081 gatcttctcc gtgcactctg cagatttctt ccctttgaaa aagtctgaaa gcctacttat
     1141 ccagttcggc catcttgttt cctatgtctg catcattata aattgcagaa tttttatcca
     1201 tttttgaata tattttcatt gctttttttc ttgaggtttt tgcttcttca agcattgagt
     1261 tgcggatggc aagtctgaaa acggtcagat agacctctcc ggcaaatgcg gggtgcagaa
     1321 cttcagcata ctgttcaatt ttatccatca gaaagcggat caggaatttc tgcagatttt
     1381 catcctggct cactgtcgca ttcagctgat taaaaagagt gaacaccctt ttttcctcaa
     1441 aaaaaggaaa ggacgaatac agttcaagag aaggaaaaaa actctttgtg aatgaactca
     1501 tattgccgag cataagtgca gcatgagtgt aactcagtgc agcaggcgga aaaaatggat
     1561 tgatggagat ggactttctg aagtacttcc tgcttttttc atactgtcct tcctcaaaca
     1621 gtgattctcc tttttcataa taactcagat aatctccctt gttcatccag aaatcggatc
     1681 cgtaccttct gcttttttcc atataccatt tcagagaggc ataggccata tgatgggaga
     1741 aatcatgttt gtgattcttg aagcttttga atgttgaatc cgtctttttg gaaaagagct
     1801 tggacttttc tgaaagatac agcttaattt tcttttcagc atcccctgta tctcctgcaa
     1861 gaaagagcac tttcagttta gatacatttt ccgagttttt ttcatccgga gtgatgtttc
     1921 cgcctgatac atttgcggaa aacggaaaga ggtcggggtt tctgactgct gtcagaagtg
     1981 cctgacttgc tccctgtgaa tacccgatga gaagggtttc cggcatcagg ttgtacttgc
     2041 ttcgtatcct gtgaactgtt tccagaatat aggattcact ttcagagtaa ccccatttcc
     2101 tggcgttcct ttccggatcg ggaatatttc cgtcaatgca gagaagaacc atattcatat
     2161 atctggtata attagaaagt acaccagcca tttcggcact gttcccgcga agaccgtgaa
     2221 gacagacaac cagaatgtat tttctggagg aatcaaagcc gaccggctca actacagtgt
     2281 agctgtattt tctgtcgtcc gctgcccgaa gcggcttctg ctgagccagg acagagacgg
     2341 aaagaaatgc aaatataatg aaaaactgaa aaaatttcat caaatgtccc ataacgatga
     2401 tccgctgctg caaaaatcct actgaaccgg gatttccgat ctgaatacga catcctcaaa
     2461 aaaacctgtt cctttgctgc cctttatccc aagaattttt tttctgatct gagattctgt
     2521 gattccgtat ttttttacca gttccctgtt caggctgatc tgtcctactg tcataaaagc
     2581 agcaagggac tctttttcct caggaggaag aaggctgttc agtgctgaga acaccttgtt
     2641 tacggtcagc ctctgccctc ccgaaatctc agggagcggt gcgcatccgt gatctgc
//
LOCUS       DAOQGJ010000347         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23582_length_2695_cov_2.863258, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000347 DAOQGJ010000000
VERSION     DAOQGJ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23582_length_2695_cov_2.863258"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..2057
                     /locus_tag="PK683_06735"
     CDS             183..2057
                     /locus_tag="PK683_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH08180.1"
                     /translation="MSDKIQIKKLPEYIYQLLNKNFIPLNFKLTSVNSLIMIAAMSLM
                     IFIATQYFKKDSELRIRENNVMITDAIGARVNAELASILQTVRFTVADMEERYSAKTL
                     DQYDSHIISIGIYQSTGYALRPIRTVYSRKILAEAELTEPDIENQIQIYTKHLEKALE
                     SDSSLQNFSPGLKFPAFAVSIPYRKDRNQIVIACIDYKKIRNSFETKGSISETLLVNA
                     SGNVIGHPDQELIIKGENLYDLPVVREMFASNAGSGQIIYAKNGENFIGSYKKVGFAN
                     AGVVSIISKDRALEEVYNIQRRNIILMIISILIVFFVVYNFAKQITRPVLHLVRLARQ
                     IQEGNFNVSVAYRSKDEIGLLEEAFQEMSEGLAEREKVKDALTKFVSKEIAEKALKGE
                     IKLGGEQKVCVIFFSDIRGFTAMSEKLSPEEVVELLNDYLTRMVDCVNLSHGYVDKFI
                     GDAIMATWGVPISRGSDAENAVNGALHMRSALMEMNASRKAEGRPELRIGCGLNYGAV
                     VAGQIGSNEKLQYTVIGDSVNLASRVESLNKPFGTDILITQDLYDQVQNIYRTEKMQQ
                     IKVKGKSEPQTIYAVLGRYDDPNCPETLDELRALLGIKHDPSSEGLSAAENEVKYEII
                     "
     gene            2044..>2695
                     /locus_tag="PK683_06740"
     CDS             2044..>2695
                     /locus_tag="PK683_06740"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH08181.1"
                     /translation="MKLYNFNIFKEISPEEERKDQRKTNTATLSLIFLLVCFLYWDSN
                     KDPEIGNRKLVGKVASAVNQTERKLGATVVWRKIIKDYSVYNYDTIRTEADSKTVIEL
                     TDGTKVTLNEDTIIYINTNPEKLDINLIQGSMTVDTMNRKESEVEKINIVHGNEVIKV
                     DRSFLSISKKKSSSSLNLELKEGEALLRKEDKEYILQKGNTVISRKDFIKELNIQYQ"
BASE COUNT          887 a          464 c          621 g          723 t
ORIGIN      
        1 aactgtgaag gtgataatat ctcaccttgc gcagagagga tatactgtaa tttttcagaa
       61 ttatcagaga gatcaaatga accgactgaa gcgggatttc aggcagcttc atttttacga
      121 aatcagcttc ccgaacagcg agttatatat ttttttacaa taatgaggta tctggaaatc
      181 agatgagtga taagattcaa ataaagaaat taccggaata catatatcag cttttaaaca
      241 aaaattttat ccctctgaat tttaaactga cttcagttaa ttctctcatt atgattgcgg
      301 caatgagtct gatgattttt attgctactc agtattttaa aaaggacagt gaactccgta
      361 ttcgggaaaa caatgtgatg atcaccgatg ccatcggagc aagagtaaat gcagagctgg
      421 catccatact gcagacagtc cgatttaccg ttgcagatat ggaagagcgg tattcggcaa
      481 agacactgga tcagtatgac agtcatatta tctcaatcgg catatatcag tcaaccgggt
      541 atgcacttcg tcctattaga acagtttaca gtcgaaaaat actggctgaa gcagagctga
      601 cagagccgga tatagaaaat cagattcaga tctataccaa acatctggaa aaggctttgg
      661 aatcggattc cagtcttcag aatttcagtc ccggacttaa gtttcccgca tttgctgttt
      721 ccattccgta cagaaaagac agaaatcaga ttgtaatcgc ctgtattgat tacaaaaaga
      781 tcaggaattc ctttgaaaca aagggaagta tttcagaaac actgcttgtc aatgcttcag
      841 ggaatgtaat cggacatccc gatcaggaac tgattatcaa gggcgaaaat ctgtatgatc
      901 tgccggttgt gagagagatg tttgcatcca atgcgggcag cggccagatc atctatgcca
      961 aaaatgggga aaatttcatc ggctcttata aaaaagtcgg attcgcaaat gcaggtgttg
     1021 tatccattat atcaaaagac agagctctcg aagaagtgta taacattcag agaagaaata
     1081 ttatattaat gattatatct atcttgattg tattttttgt ggtatacaat tttgcaaaac
     1141 agattactag accggttctg catttggtcc gtcttgcaag acagatacag gagggaaatt
     1201 ttaatgtttc cgtggcatac agatccaagg atgaaattgg acttctggag gaagcttttc
     1261 aggagatgag tgaggggctt gctgaacggg aaaaagtaaa agatgcactg acaaaatttg
     1321 tttccaagga aattgcagaa aaagctctca aaggcgaaat taagctggga ggagaacaga
     1381 aggtctgtgt aattttcttt tctgatattc ggggctttac ggcaatgtct gagaaactgt
     1441 cccccgagga agtggtggaa cttctgaatg attatctcac acgtatggtt gactgtgtaa
     1501 atctttcaca tgggtatgta gacaagttta tcggagatgc tattatggcg acttggggcg
     1561 tgcctatttc ccgagggagc gatgcagaaa atgcagtgaa tggagctctg catatgcgga
     1621 gcgcactgat ggaaatgaat gcttccagaa aagcagaagg cagaccggaa ctccgtatag
     1681 gatgcgggct gaattatggg gcagtggttg ccggccagat tggttctaat gagaaactgc
     1741 agtatacagt gattggtgac agtgtgaatc tggcatccag agttgagtct cttaacaaac
     1801 ctttcggcac agatatactg attactcagg atctttatga tcaggttcag aatatatatc
     1861 gaacagagaa gatgcagcag attaaggtaa agggaaaaag cgaacctcag accatttatg
     1921 cagttttggg aagatatgat gatccgaact gtcctgaaac tctggatgag cttcgagctt
     1981 tactaggcat aaagcatgat ccgtcttccg agggtctgtc tgcagctgaa aatgaagtaa
     2041 agtatgaaat tatataattt taacatattt aaggaaatca gtccggaaga agaaaggaaa
     2101 gatcagagaa aaacaaatac cgcaacactt tcccttattt ttcttcttgt ctgttttcta
     2161 tattgggaca gcaataaaga tcctgaaatc ggaaacagga aactggtcgg aaaagttgct
     2221 tctgctgtca accagactga gcggaaactg ggtgcgacag tggtctggag gaagatcatt
     2281 aaggattact ctgtttataa ttatgacacg atcagaactg aagcagattc caaaactgtc
     2341 attgaactga cagacggaac aaaagtcact ttaaatgaag atacgattat ctacattaat
     2401 acaaatccgg agaaactgga catcaatctg attcagggat ctatgactgt tgatacgatg
     2461 aacagaaaag aatctgaagt ggagaagatc aatattgttc atgggaatga agtgattaag
     2521 gttgacagaa gttttctgag catcagcaaa aagaaatcca gttcttccct gaacctggaa
     2581 ctgaaagaag gagaagctct tctcagaaag gaggacaaag aatacattct tcagaaaggg
     2641 aataccgtta tttccaggaa ggacttcatc aaggaactga atatccagta ccagg
//
LOCUS       DAOQGJ010000348         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23594_length_2694_cov_3.311482, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000348 DAOQGJ010000000
VERSION     DAOQGJ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23594_length_2694_cov_3.311482"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PK683_06745"
     CDS             complement(<1..1048)
                     /locus_tag="PK683_06745"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNH08182.1"
                     /translation="MLNKIFPVQFSYLPIPIKTIGIFFLLIPFISFIATAELSFADPN
                     AVNIKGFSQRISDISSRFDYVSISLNIVSIIVGLGILGVKRWGYFLFLAFFSVLIGHG
                     VFLLAKFGFTEDFIQNLLVTLLPFFVILYFLNQEISTPYLTLLPRGFRNKWRIEIPLK
                     GSVTLSSGKKIPLVTMDISPSGCLAKTEETVAMDEKVTLTLELDSPWTVEAENVREVE
                     GNFGFKFRLRRGDSNYKQLDKYLSTKLVPRFDVFKASAEIEQDGKKFTADVVNISDGG
                     FYLATKEVLTVDSRVKFDMVLKGIKFRGAGKVSWENQAEKFDKPAGYGIAIKAMNREI
                     FYKTYLFVLTQKRNF"
     gene            1301..>2694
                     /locus_tag="PK683_06750"
     CDS             1301..>2694
                     /locus_tag="PK683_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08183.1"
                     /translation="MESINNLVIGKNSLKIRLIIALLSINLLILLYGGISIYLVNELY
                     AQAKITTSQTLPLVQQTNEFNQELSRFRRNLSKATLAADQNEQNSIKLKLTEGRQKIL
                     QIGRNLEQFANTEGERASLKEMEKAVNSYFEDTEDMIRISNSSFDPTLKMKLTSNSLS
                     KFEELMMRLQTIEDEFGKNSLKASENLTVLFASNQSVTGVTLFLITVFLVLLSIRTLT
                     TILRPINRSIELVTDYSKGNFTAEIPEEKTLEYGKIFTAFGNMAARLSDVIRNIRKVS
                     AGILKSSGSLVGRSDTLSRLSQDMAASSEESSASIEELSTSLENVGKSITSQTKNMYE
                     IESNINGFTESIDEIKKSSERLAMISNDAAKRASGGESTIRETSAAMDKIKMSSSKIT
                     EIVGLISNISSQTNLLSLNAAIEAARAGEAGRGFAVVADNISKLADRTVHGVKEIQTL
                     IADTDAAIKDGFIKV"
BASE COUNT          851 a          553 c          543 g          747 t
ORIGIN      
        1 cgaaatttcg tttctgggtc agaacaaaaa gatatgtctt ataaaaaatc tctctgttca
       61 ttgccttgat tgcaattcca tatccggccg gtttatcaaa tttttcagcc tgattttccc
      121 agctcacttt gcctgctccg cgaaatttaa ttcctttcag gaccatatca aattttactc
      181 tgctgtctac ggtcagaact tcttttgtag caagataaaa tcctccgtca gaaatgttca
      241 ccacatctgc tgtaaatttc tttccatcct gctctatctc ggctgaagct ttaaacacat
      301 caaatctagg aacaagtttt gtggaaaggt acttatccag ctgtttataa ttggaatctc
      361 ctctcctgag cctgaactta aatccaaaat tgccttccac ttcgcggaca ttttcagcct
      421 ccactgtcca gggagaatcc agctctaaag tcaaagtgac cttttcatcc attgcaactg
      481 tttcttcggt ttttgcaagg cagccgcttg gactgatatc catagtgact aacggaatct
      541 ttttaccgga agaaagggtg actgaacctt tcaggggaat ttctattctc catttatttc
      601 tgaaaccgcg aggaagaaga gtcaggtaag gtgtagatat ttcctgattc aggaaataaa
      661 gaattacgaa aaaaggaagc agcgtcacaa gcagattctg gataaaatct tcagtaaatc
      721 cgaatttagc aagcaggaat accccgtgcc cgatcaggac ggaaaaaaat gcgagaaaca
      781 gaaagtagcc ccatcttttt actcccagga ttccaagtcc tacaataatg ctgactatgt
      841 tcagagaaat actgacataa tcaaatcggg aagaaatatc actgattctc tggctgaacc
      901 ccttgatatt cactgcgttt ggatcagcaa aactcagctc ggctgtagca ataaaactga
      961 tgaatggaat cagtagaaaa aagatcccta ttgtttttat tgggataggt agatatgaaa
     1021 attgaaccgg aaatatttta ttcagcacgt tatgccctct ctcgatcaga aaaaatagtt
     1081 ttttttaagt aaaaaaagtg gcaagctttt tcggattttc agatctgatt ttttactgca
     1141 aacctttcac gctcccgata ataaataagg cggatacagt ttctcagtat ccccagaaaa
     1201 gaattgctca actgcatcga aaaacctgct tttcatccgg tgctgaccga atgtttcgcg
     1261 gtaatgggtg cggtttgagt aaatctaggg aagtggacgg atggaatcaa taaataattt
     1321 agtaatcggt aagaacagtt taaaaatcag actgatcatt gctcttctga gtataaatct
     1381 tcttatattg ctatatggag gaatttccat ttatcttgtc aatgagctgt atgcacaggc
     1441 aaagattact acctctcaaa cccttccttt ggtgcagcag acaaatgaat ttaatcagga
     1501 actttcccga ttcaggagaa atctttcaaa ggcgacttta gccgctgatc agaacgaaca
     1561 gaattccatt aaattaaaac tgacggaagg aaggcagaag attctgcaga ttggaagaaa
     1621 tcttgagcag tttgcaaata cggaaggaga gagagcttcc ctaaaggaaa tggaaaaagc
     1681 agtcaattcc tactttgaag atactgagga tatgatccga atttccaatt ccagttttga
     1741 tcctacctta aaaatgaaac tgacttcaaa ttccctctcc aaattcgaag aactgatgat
     1801 gcgtcttcag acaattgaag atgagttcgg aaagaacagt ctgaaggcat ccgagaatct
     1861 gacagtcctt tttgcttcaa atcaaagcgt gacaggagtc actttatttc tgatcactgt
     1921 ctttcttgta ctgctgagca tcagaacact gactacaata ctccgtccaa tcaacagatc
     1981 cattgaactg gtcacagatt actcaaaagg aaattttact gctgagatac ctgaggaaaa
     2041 aaccttggag tacggaaaaa tattcactgc cttcgggaat atggctgcaa ggttaagtga
     2101 cgtaattcgg aacatccgga aggtatctgc cggaattctg aaatcatccg gttcgcttgt
     2161 cggcagatcg gatacgcttt ccagactttc tcaggatatg gctgcttctt cagaagaatc
     2221 gtctgcatcc attgaagaac tctcaacttc cctggaaaat gtaggaaaaa gcattacttc
     2281 tcagacaaaa aatatgtatg agattgaatc gaatatcaac ggtttcaccg aatctattga
     2341 cgagatcaaa aaatcatcgg aaagactggc tatgatcagc aatgatgccg ccaaaagagc
     2401 atcgggcggg gagagtacta ttcgggaaac cagtgccgct atggacaaaa tcaaaatgag
     2461 ctcctcaaaa atcacggaga ttgtcggatt gatatccaat atatcttctc agacaaatct
     2521 gctgtcctta aatgccgcaa ttgaagcagc gagagcagga gaagcaggaa gagggtttgc
     2581 ggtcgttgct gacaatattt ccaaacttgc agacaggaca gttcacgggg taaaggaaat
     2641 tcagacgctc attgctgata ctgacgcagc catcaaagac ggttttatca aggt
//
LOCUS       DAOQGJ010000349         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23805_length_2678_cov_2.763248, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000349 DAOQGJ010000000
VERSION     DAOQGJ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23805_length_2678_cov_2.763248"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1268)
                     /gene="hemW"
                     /locus_tag="PK683_06755"
     CDS             complement(105..1268)
                     /gene="hemW"
                     /locus_tag="PK683_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNH08184.1"
                     /translation="MNTFSPLVCRKGYGGIYIHYPFCVHKCAYCDFYSAGIGLGKSGK
                     DEELFSAFRNETDFRLRQFPEARNLVYDTVFFGGGTPSKADPEEIHKFLYFLKDRLHI
                     APDAEITVECNPEDLTAELLRSYSEAGITRINAGVQSFSPVHLKTLDRHYRGEKYGQI
                     LEILKNSPFSKKGIDLIYGIPGQTMNDFFSDVKTAAENGLSHISAYSLTAEKGTEYYR
                     KIGTGQSLSPNEELQSEILHLLPKFLSDFNYRQYEVSNYSISGYESFHNLKYWLMEEY
                     LGIGPGAHGFIGNRRYSNVRSTDVYLRMNWSSDSEISNLPAELALCLFRIFIPIRLNG
                     FLDRFLPEKKDPALSLIENWKNEGYCTWENGVFEWKQRAVLYLDDRISEFAGL"
     gene            complement(1265..1873)
                     /locus_tag="PK683_06760"
     CDS             complement(1265..1873)
                     /locus_tag="PK683_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08185.1"
                     /translation="MNSSENEFGCTYLSEEDAVSVLDEFFSALTDRTVSFDQESVSEY
                     FRKNAFLTKDAADSLAKALISSISDPEEIQYSVVFKKKFRFKDFLLWDKEVYEAAEEF
                     KKEFSLYPVLLAAAPQTLSRIDMIVNFGSRDRIKNTENSSLEKGEFAQLGGFSSDNFE
                     LDFAADSELEDREFLLIFDSDPDWGGESKDIPEDAEEESKAA"
     gene            complement(1895..>2678)
                     /locus_tag="PK683_06765"
     CDS             complement(1895..>2678)
                     /locus_tag="PK683_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08186.1"
                     /translation="AEGAEAAVTRAVLAVLAGADVILMTSFGKNTAEYYDMVLSAVKK
                     KEFMSGEINLIDEAIKRQLIAKMELGLFSESFSSLNINDEELKTYLSRRNKSAKEFYE
                     YLKKENLNDLNRKISSEGIRAWKNEFFPPSAETVKTFHFHLRQDRLKEEVELLGYDAV
                     SEKRLKKMLSKKIKGVFVLDSRDQADLRRINSFIKTNPKADYILLHTGSPFLEFPSNP
                     NVKVLFSFSPTDASCAGLVLRAFNSEESVKPADIVYRKTMLK"
BASE COUNT          732 a          604 c          532 g          810 t
ORIGIN      
        1 cggtcttctg attgaagcaa attccaaact tgataaggtt tcagcacaga tcaaaaaaga
       61 caagaaaaaa gataagaaaa agtaatatac tcattgccgg ttttttacag accggcaaat
      121 tctgagatcc tgtcatccag ataaagcaca gccctttgtt tccattcaaa taccccgttt
      181 tcccaggtgc agtatccttc gtttttccag ttttcaatga gagatagagc agggtctttt
      241 ttttccggca gaaatctgtc caggaaaccg tttaatctta tcgggatgaa aatccggaaa
      301 agacacagtg cgagttcagc cggaagattc gatatttcag agtctgaact ccagttcatc
      361 ctcagataca cgtcggtcga tctgacattt gaataacgtc tgtttccgat gaagccgtgt
      421 gcacccggcc caatccccag atactcttcc ataagccagt atttcagatt gtgaaacgat
      481 tcataacctg aaatgctgta attggagact tcgtactgtc tgtaattgaa gtcagaaaga
      541 aatttcggca gaagatgcag aatttccgac tgaagctctt catttggaga aagggactgt
      601 cctgttccga ttttcctgta gtattctgtc cctttttctg cagtgagaga ataggcactg
      661 atatgagaga gaccgttttc tgccgcagtt tttacatctg aaaaaaaatc gttcatcgtc
      721 tgtccaggta tcccatagat cagatctata cctttctttg agaagggaga gtttttcagt
      781 atttcgagaa tctgtccgta tttttcaccc ctgtaatgtc tgtcgagagt cttcagatgc
      841 acggggctga aactttgaac acctgcattg atccttgtga ttccagcttc agaatacgac
      901 ctcagaagtt cagcggtcag gtcttcagga ttgcattcca ctgtgatctc tgcatccgga
      961 gcaatatgaa gtcggtcttt cagaaaatat agaaatttat gaatctcttc aggatctgct
     1021 ttggacggag tgcctcctcc gaaaaaaact gtatcgtaga caagatttcg ggcttcagga
     1081 aactgcctta gtctgaaatc agtttcattc cggaaggcag agaaaagctc ctcatccttt
     1141 cctgattttc ccagaccgat tcctgcagag taaaaatcac agtatgcaca tttatggaca
     1201 cagaaaggat aatgaatata tatccctcca tatccttttc tgcatacaag cggagaaaat
     1261 gtattcaagc ggcttttgac tcttcctctg catcctcagg gatgtcttta gactctccgc
     1321 cccagtcggg atctgaatcg aagatcagaa gaaattccct gtcctccagt tctgagtctg
     1381 ccgcaaaatc cagttcaaaa ttgtctgaag aaaatcctcc cagctgtgca aattcccctt
     1441 tttccaggga agagttttct gtatttttga tcctgtctct gcttccaaaa tttacgatca
     1501 tatcaatcct ggaaagagtc tgaggtgcgg cggcgagaag gacagggtag agagaaaact
     1561 cctttttgaa ttcctcggca gcctcataca cttccttgtc ccagagaaga aaatctttga
     1621 atctgaattt ctttttgaat acgacagaat actgaatttc ttcaggatcg gaaatactgc
     1681 tgatgagtgc ctttgcaaga gaatcagcag catcctttgt cagaaatgca ttttttctga
     1741 aatactcaga aacggattcc tgatcgaatg aaactgtcct gtcggtcagt gccgaaaaaa
     1801 attcatcgag tacagagact gcatcctcct cactgagata agtgcatccg aattcattct
     1861 cagaactgtt cacgtattcc cgccgcctat atcattattt cagcattgtt tttctgtata
     1921 caatatctgc cggtttcact gactcttccg aattgaatgc tctcagtacc agacctgcac
     1981 aggatgcatc tgtaggagaa aaggaaaaga gcaccttgac attcggattg gaggggaatt
     2041 ccagaaaagg agatccagtg tggagcagaa tgtaatctgc cttgggattt gtctttataa
     2101 aagaattgat tcttctcaga tccgcctgat ctcttgaatc caggacaaac actcccttga
     2161 ttttttttga aagcattttc ttcagacgtt tttcgctgac tgcatcatat cccaggagtt
     2221 ccacttcttc cttcagtctg tcctgtctca gatgaaaatg gaatgttttt accgtttcag
     2281 cagaaggggg aaagaattca tttttccagg ccctgatgcc ttcagaagaa atttttctgt
     2341 tcagatcgtt cagattctcc ttcttcagat actcgtaaaa ttccttagcc gacttgtttc
     2401 ttctggaaag atatgtcttc agttcctcat cattaatatt taatgatgaa aatgattcac
     2461 tgaaaagtcc cagttccatt tttgcaatca gctgtctttt gatcgcctca tcaatcagat
     2521 tgatttctcc cgacataaat tcttttttct tcactgcaga aagaaccata tcatagtatt
     2581 ctgcagtgtt tttgccgaaa cttgtcataa ggatcacatc cgctccggcg aggactgcca
     2641 gaactgcccg agtgactgcg gcctctgccc cttctgcg
//
LOCUS       DAOQGJ010000350         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23814_length_2677_cov_3.403509, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000350 DAOQGJ010000000
VERSION     DAOQGJ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23814_length_2677_cov_3.403509"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PK683_06770"
     CDS             complement(<1..990)
                     /locus_tag="PK683_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNH08187.1"
                     /translation="MKTYISIFLLTGFFLPASAQSGTGRLHPLFPLLDEKGTHVRVSK
                     AEISTAKTCGGCHDTDYIRKNSHHFNNKVKADCIQCHTENGRIDWKPNYFSSSGELLR
                     EFISIRSPENRNCGSCHGVVHQEQIPLELPSDFLSPGKYESTKRTGEIISYQKLHSSF
                     LNLQNKSKTEFSWDIHAERKVDCVDCHYSSNNPKKTLPKKDNDLKHLVQDPRTPSIAE
                     FLNRPDHRLTTSGCSSCHNPLVIHDMIPYKKKHMERVECQTCHVPVLKGPALSEIDAT
                     VVLENSTPSYKFRNINYTSEKLLNAEYISDFVPYMLPSSDSKIKPFNLVTEWSW"
     gene            complement(1009..2460)
                     /locus_tag="PK683_06775"
     CDS             complement(1009..2460)
                     /locus_tag="PK683_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HNH08188.1"
                     /translation="MQVNEKNWILGVILTAFIFLFSLYFFWPGGVRAEKPISSFEVPQ
                     RIHFSHSMYFEEFVAKGFPSPQDVTKACLKCHPKSAENLMQTSHWTWLRSSKKLAKNS
                     QPAEIGKKNLLNNFCIGITGNWPSCTKCHAGYGWEDHTFDFKKSENVDCLVCHDWSGT
                     YTKGASGVPPKEVNLLTAARSVGYAKRDNCGMCHFSGGGGMAVKHGDLDDSLIYAVEQ
                     VDVHMGKNKMLCTDCHRTKNHSVPGTAFSVSAEQANAVDCTNCHTENVHKNERINSHV
                     KSVACQTCHIPNYAKRTPTKMTWDWSKAGDAKRKEDPHSYLKIKGEFTYAMNQKPEYY
                     WFNLTVERYLMGDKIDPEKMSNINYPSGDINDRKAKIWPFKIHKAKQPYDKKLNTILP
                     VVTAGEGGYWQKFDWKKALQLGAEQNKTEFSGEYGFAETQMFWPLSHMVSPAKDSLGC
                     NDCHGNPSRMDWKALGYSGDPAISGSRDFSKLK"
BASE COUNT          699 a          607 c          552 g          819 t
ORIGIN      
        1 ccagctccac tcagtgacaa gattgaaagg cttaatcttg gaatcggaag agggaagcat
       61 atacggaaca aaatcgctga tgtattctgc attcagaagt ttttcagatg tataatttat
      121 attcctgaat ttatatgacg gagtggaatt ttccagaaca actgtagcat cgatttcaga
      181 cagtgcaggt cccttcagaa caggcacatg acaggtctga cattccactc tttccatatg
      241 cttctttttg taaggaatca tatcatgtat cacgagagga ttgtgacagg aagagcatcc
      301 ggaagtggtc agacgatgat ccggtctgtt cagaaattct gcaatgctcg gagttctggg
      361 atcctgcaca aggtgcttca gatcattgtc ctttttagga agggttttct tcggattatt
      421 tgaggaataa tgacagtcta cacagtccac ttttctttcc gcatgaatgt cccaggagaa
      481 ttctgtcttt gatttgttct gaagattcag aaatgaagaa tggagtttct gataggatat
      541 gatctctccg gttcttttcg tagactcata ttttccggga ctcaaaaaat cagaaggcag
      601 ttccagagga atctgctcct gatgaaccac tccatgacag gaaccgcagt ttctgttttc
      661 aggagaacgg atggaaataa actctctgag gagctctcct gaactgctga aataattcgg
      721 cttccagtca atccgtccgt tttcggtgtg acattgaata cagtctgctt tcaccttatt
      781 attgaaatga tgactgttct ttctgatata atcagtgtcg tgacagcctc cgcaggtctt
      841 tgcagtggat atctcggcct ttgagactct tacgtgagtt cctttctcat cgagaagagg
      901 aaaaagggga tggagccttc cagtaccgga ctgagcagat gccggcagga aaaaccctgt
      961 cagcagaaaa atactgatat aagttttcat aaaatctcct gacatatatt atttcagttt
     1021 agaaaaatcc cggcttccgg aaatggcagg gtctccggaa tatcccagag ccttccagtc
     1081 catccttgaa ggatttccat gacagtcatt gcatcccagg ctgtcctttg caggactgac
     1141 catatgggaa agaggccaga acatctgtgt ctctgcaaaa ccgtattcac cgctgaattc
     1201 agttttgttc tgttctgctc ccagctgcag tgcctttttc cagtcaaact tctgccagta
     1261 tcctccctct cctgcagtca ccacaggaag aatcgtattc agttttttat catagggctg
     1321 ctttgccttg tgtatcttga acggccagat cttagccttc ctgtcattta tatccccgga
     1381 aggataattt atattgctca ttttttcggg atcgatttta tctcccatca gatatctttc
     1441 cacagtcaga ttgaaccagt agtattccgg tttctgattc attgcataag taaactcacc
     1501 cttgattttc agataggaat gaggatcttc ttttcttttt gcatctcctg cctttgacca
     1561 gtcccaggtc attttcgtcg gagtcctttt tgcatagttc ggaatatggc aggtctgaca
     1621 ggctaccgat tttacgtgag aattgattct ttcatttttg tgaacattct ccgtatgaca
     1681 gttggtgcag tccaccgcgt ttgcctgttc tgcagaaacg gaaaaagcag tcccgggaac
     1741 agaatggttc tttgtcctat ggcagtcagt gcagagcatt ttattttttc ccatatgcac
     1801 atccacctgc tccactgcat aaatcagact gtcatccaga tcaccgtgtt tgaccgccat
     1861 ccctcctcct ccggaaaaat gacacatacc gcagttgtct cttttagcat aaccgacaga
     1921 tcttgctgcc gtaagcagat tcacttcttt gggaggaact cctgaagctc ctttagtgta
     1981 agtgcccgac cagtcatggc agacaagaca gtccacgttt tccgattttt tgaaatcaaa
     2041 agtatggtct tcccatccgt accctgcgtg acattttgta caggaaggcc aattccccgt
     2101 aatgcctatg cagaaattat tgagcaggtt cttttttccg atctcagccg gctgagaatt
     2161 cttagcgagc tttttgctcg accgaagcca ggtccaatgc gaagtctgca tcagattttc
     2221 tgcagattta ggatgacatt ttagacaggc cttagtgaca tcctgaggag aaggaaatcc
     2281 ctttgctaca aactcttcaa agtacatgga atgactgaaa tgaattctct gcggaacctc
     2341 aaaactgctg atcggctttt ctgctctcac cccgcccggc caaaaaaaat acaaagaaaa
     2401 caggaaaata aaggcagtca gaatgactcc gagtatccag tttttttcat ttacctgcat
     2461 ataagcaccc ttgtctgctg tctatctcag cacctgttac cacaatcgga ggttctttct
     2521 gaaattacaa ttcattctcc ataaaagaga gatgaaaaat atcatagtgt gaatttatac
     2581 ctttacgggt atgggtattt tgagactggc aatcatttat ccgttatact cgttttcaaa
     2641 agtctttgcg tatttatttg ttcacgactc agtaaaa
//
LOCUS       DAOQGJ010000351         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23842_length_2676_cov_2.506295, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000351 DAOQGJ010000000
VERSION     DAOQGJ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23842_length_2676_cov_2.506295"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..861)
                     /locus_tag="PK683_06780"
     CDS             complement(22..861)
                     /locus_tag="PK683_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HNH08189.1"
                     /translation="MIKKSVSIETRVFDLDWNRHVTSRTYERFSLEGRHSILADLGYP
                     IQKCIQENLILIPKFTEVRFLAQQFSGTVLRVDTVMEEYRNGLIFWDHKIYDPSEKLA
                     CELKLVTQTQDGKGQTFLFTKTDLTEPENLIHSVRQFTGSCRRLENPFRMLFSDMSCF
                     WVYPAESVWKIFEEGRWLFFGDIVDTKKIKEHDTTSFFMGGKIQIFRLPEAGEKAVLY
                     SWIESVDKIRFYFRQDLADLKGNVFASMRDEQLFVSLSASRPRKAPQYFLETVGEYVE
                     FLK"
     gene            complement(1248..2594)
                     /locus_tag="PK683_06785"
     CDS             complement(1248..2594)
                     /locus_tag="PK683_06785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH08190.1"
                     /translation="MAKLSKWGVGACLADDMGLGKTIQALAMLLTYAPKGPSLVIAPT
                     SVCMNWHSEAARFAPTLNTIQFGAGDREKIIKNLKSFDLLICSYGMLQQTEAADLLKD
                     TDWKMVILDEAQAVKNMTTKRSQAVMNLKADFKLITTGTPIENHLGELWNLFRFLNPG
                     LLGSIEKFNEKYAVPIERNKDKYARNRLKRIIQPFILRRMKNDVLEELPEKTEIVIQV
                     EQSGREKAFYEALRQNAVKKLSGEDMHKGQKHIQILAEIMKLRRACCHSSLVDKKIDI
                     PSAKFEAFTEILEELLANRHKALVFSQFTDHLAIIKKHLDTLKIHYQYLDGSTPAGER
                     KKRVEKFQSGEGDVFLISLKAGGAGLNLTAADYVIHMDPWWNPAVEDQASDRAHRMGQ
                     KRPVTVYRIVAKDTIEEKIVELHKHKRDLADSLLEESEMSGKMSADELLKLLRGEE"
BASE COUNT          674 a          686 c          533 g          783 t
ORIGIN      
        1 ttataacctg acaaaatcgc attatttcag aaactccaca tactccccca cagtctccag
       61 aaaatactga ggcgcctttc tcggtctgga tgcagagagg cttacaaaaa gctgctcatc
      121 tctcatcgat gcaaatacgt ttcctttcag atcagccaga tcctgacgga aataaaatcg
      181 gatcttgtcc acagactcta tccaggaata cagaacagcc ttttcacccg cttcaggcag
      241 tctgaaaatc tggatttttc ctcccataaa aaaagatgta gtatcatgct ctttaatttt
      301 ttttgtatct acaatatccc cgaagaaaag ccatcttcct tcttcaaaaa ttttccagac
      361 ggattctgca ggatacaccc agaagcagct catatcgctg aacagcattc ggaaaggatt
      421 ctccagcctt ctgcaggaac cggtaaactg cctgactgaa tgaatcagat tttccggctc
      481 tgtaagatct gtctttgtaa acagaaaggt ctgtcctttt ccatcctgag tctgcgtcac
      541 aagcttgagt tcacaggcaa gcttttcaga agggtcataa attttatgat cccagaagat
      601 aagcccgttt ctgtattcct ccatcactgt gtccactcgc agaactgtcc cggaaaactg
      661 ctgcgcgaga aaacgcacct cggtaaactt aggaatgaga atcagatttt cctggatgca
      721 tttctgaata ggataaccca gatccgcaag aatggaatgt ctcccctcca gactgaaccg
      781 ctcataggtt ctgctcgtca catgcctgtt ccagtccaga tcaaacaccc ttgtttcaat
      841 tgagacagat ttctttatca tgataattca gatttttccc ggacgcttcc gctgtcaagc
      901 gttgagcagg gacgggaggg aggctgaaac caaaacaaga cggagagagg agtttatata
      961 aatgcttatt atttgagagt gcagaatgaa ggtgtcagtg cagggctgga ttcaaagaat
     1021 ggtttcacac ggggatgcag atatttatag taaataaata tatctttcca tactggccat
     1081 tttacgcttt gcagcaaaaa ttatattctc attataaaat cagcattcgg aaaaaagacg
     1141 ggatagggat tgaggcagcg aagcggtgtc aattttatgc ttatgcagaa aattgacaag
     1201 agccgaaagc ccggtcggca catctgtttg cgccgaatcc cccagaatca ttcttcgccg
     1261 cgaagaagtt tcagaagttc atcagcagac atcttgccgc tcatttcgga ttcctcaagc
     1321 aggctgtccg cgaggtcgcg cttgtgcttg tgcagctcca cgatcttttc ttcaatcgtg
     1381 tccttggcca caatcctgta aacggtaacc ggccttttct gccccattct atgcgccctg
     1441 tctgaagcct gatcttctac agcaggattc caccagggat ccatatgaat cacatagtcg
     1501 gccgctgtca gattcagtcc tgccccgcct gccttcagac tgatcaggaa aacatcccct
     1561 tctcctgact gaaatttctc cactctcttt tttctctccc ctgcaggagt gcttccgtcc
     1621 agatactgat aatgtatttt taatgtatcc agatgttttt taatgattgc aagatgatcc
     1681 gtaaactgac tgaataccag tgccttgtgc cggtttgcca gaagctcttc caggatttcc
     1741 gtgaatgcct cgaacttcgc actggggatg tcaatttttt tgtccacaag actcgaatga
     1801 cagcaggctc ttctgagctt cataatctcc gcaagtatct gtatatgctt ctgccccttg
     1861 tgcatatcct caccgctgag ttttttgact gcattctgtc tgagtgcctc ataaaatgcc
     1921 ttctctcttc cgctctgctc cacctgaatg acaatctctg tcttctccgg aagttcttcg
     1981 agaacatcgt ttttcatcct cctcagaatg aaaggctgta tgattctttt gagccggttt
     2041 ctcgcatact tatccttgtt tctttcaatc ggaacagcat atttttcatt gaatttttcg
     2101 atcgaaccca gaagtcccgg attcagaaaa cggaaaagat tccagagttc ccccagatga
     2161 ttttcgatcg gcgtgcccgt agtgatcagc ttgaaatctg ccttcagatt catcactgcc
     2221 tgcgatctct tcgtcgtcat atttttgact gcctgcgcct catccagaat caccatcttc
     2281 caatctgtat ccttcagaag atcagcggcc tctgtctgct ggagcatccc gtagctgcag
     2341 atcagaagat cgaatgattt taaatttttt ataatttttt ctctgtctcc tgcaccaaac
     2401 tgtattgtgt tcagcgtagg cgcaaaacgt gccgcttctg aatgccagtt catacagacc
     2461 gacgtcggag caatcaccag gctgggtcct ttcggcgcat aggtgagaag catagccaat
     2521 gcctgaattg tttttccgag tcccatatca tctgccaggc aggccccgac tccccacttg
     2581 gaaagttttg ccatccattc aaagccttcc tgctggtatt ctctttttct gactgatcag
     2641 aaactctgct tccgcttttt cactctgcac aagatt
//
LOCUS       DAOQGJ010000352         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_23904_length_2672_cov_3.422621, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000352 DAOQGJ010000000
VERSION     DAOQGJ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23904_length_2672_cov_3.422621"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..749)
                     /locus_tag="PK683_06790"
     CDS             complement(33..749)
                     /locus_tag="PK683_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08191.1"
                     /translation="MITHQYFEFTPEDDRNFSKERLMSLLTDLIMRYDVSLEEALRIL
                     IEKGMTANLFLKEAGMNDLLENFLNETENEISEILKKFTLKKTIDSLQKEISETEKKN
                     SRDSYFKEFRNLLSSGSTDSFRRKLWEAPSELQGKLYQYLEELDDLNTLKNGAEKYPF
                     TGTVSPKSVRQAKKIISRLEELTELRAALREAIESGDLFNMDLGKIAETLGPESFQEF
                     LERREQIFKSIRELLEKGGY"
     gene            complement(746..1132)
                     /locus_tag="PK683_06795"
     CDS             complement(746..1132)
                     /locus_tag="PK683_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08192.1"
                     /translation="MKNILIPALFLSSVTVFGTDSPIDFELTKVKGDDYKLEIIVPKG
                     YAVQKDAPNKIELKGDDHLKVKSFKSDFKGTPMVKKPEYFEKVQSFPVKLSGKGELTI
                     NSKIFYCDLNKNVCYPAKISKKEHIK"
     gene            1282..2565
                     /locus_tag="PK683_06800"
     CDS             1282..2565
                     /locus_tag="PK683_06800"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNH08193.1"
                     /translation="MKKYTVLVAESANTDHLLINQYLRAMQFEILESGARDFGIMNSI
                     LEHSSPDILILSFDLQGMEDPFDKIREIRAKHSGIRILVIFTKIDKDVLEIMKGLGVN
                     GLMVKPVNKSSLQEKLAGMLGRNDLLPKTLEMGKESAAAKDKEAKALNDKLKIHELHI
                     PNLPEVTDKIIMFDFDPTGGSEKLEQLILPDKGLCSDILRIANSAFYGRSGKVKTLKD
                     AITLLGIRTIKNIAIVQTKKSINKNISHYPLFKKYLFELPTLTALVSFDLSVPMGLKK
                     IREELFLPASFRDSGKTILALNLTKQYHDVIEVHHKSGISLSDLEMEVFHTTSNEVGL
                     HVFKVWHMPENLYGIIQNQSFGMDQISSVQDIDRLTRLGEIISRKLLSLSVSSGETEM
                     AMSIMQIYKVHEGLIDAFDEDYYDNIRTHPFMEVL"
BASE COUNT          774 a          534 c          553 g          811 t
ORIGIN      
        1 ggaacaacgc aagctaacgg ttgaggcccg cattaatagc ctcctttttc gagaagttcg
       61 cggatggact tgaatatctg ttctcttctt tccagaaatt cctgaaagct ttctggaccg
      121 agcgtttctg caatttttcc aagatccata ttaaaaagat ccccagattc gattgcttct
      181 ctgagcgctg ctctcagttc agtgagttct tccagtctgg aaatgatttt ttttgcctgc
      241 ctgactgatt tgggcgagac ggttcctgta aacggatatt tttctgctcc gtttttcaag
      301 gtgttcagat cgtctagttc ttcaagatac tgatacagtt tcccctgaag ttcggaagga
      361 gcctcccaga gtttccttct gaaactgtca gtgcttcctg aactcagcag atttctgaat
      421 tccttgaaat agctgtctct gctgttcttt ttctctgttt cggaaatctc cttctgcaga
      481 ctgtcaatag ttttctttaa tgtaaatttt tttagtattt ccgaaatctc attctcagtc
      541 tcattaagga agttttcgag aaggtcgttc attcctgctt ctttgaggaa caggttcgct
      601 gtcatgcctt tttcgatgag aattctgaga gcttcttcaa gagatacatc atatctcatg
      661 ataagatcag tcagaagaga catcagtctt tctttcgaaa aatttctgtc atcctcagga
      721 gtaaattcga aatactggtg tgtgatcact taatgtgctc tttcttggag atctttgccg
      781 gatagcagac attcttattc agatcacagt agaaaatttt ggaattgatt gtaagttcac
      841 ctttgccgga aagtttgaca ggaaaagact gaaccttttc gaaatattcc ggttttttta
      901 ccatcggtgt tcccttgaaa tcactcttga aagacttgac tttcagatga tcatcgcctt
      961 tcagttcaat cttgttaggt gcatctttct gaactgcata accttttgga acaatgattt
     1021 ccagtttata atcatcgccc ttgacttttg tgagttcgaa gtcgatagga gaatcggttc
     1081 cgaatacggt aacagaggaa aggaaaagcg caggtattaa tatgttcttc atactagtat
     1141 tgacggcaga aaagggtcaa tagttcattt tctcagtatg aaacttgcaa aaagaaactt
     1201 tcttgaaaaa aaatcagaca tctgaatgat gaagatatgt ttacggagaa tactctctct
     1261 ttaggatttc atccgcagac gatgaaaaag tatacagttc tcgttgcgga aagtgccaat
     1321 actgaccatc ttcttatcaa tcagtatctc agggcaatgc agtttgaaat tctggaatcg
     1381 ggtgcccgcg atttcggaat aatgaattcc atactggaac acagcagtcc cgatattctg
     1441 atcctcagct ttgatcttca gggaatggag gatccattcg acaagatcag ggaaatcaga
     1501 gcaaaacatt ccggaataag gatacttgtc atctttacca aaatagacaa ggacgttctg
     1561 gagataatga aagggctcgg tgtgaacgga ctgatggtta aacctgtcaa taaaagctct
     1621 cttcaggaaa agctcgcagg aatgctgggc agaaatgatc ttcttcctaa aacgcttgaa
     1681 atgggaaaag agagtgcggc ggcaaaggac aaggaagcaa aagcactcaa tgacaaactg
     1741 aaaattcatg aacttcacat tccgaatctg cctgaagtca cggacaagat catcatgttt
     1801 gacttcgatc ccacaggagg aagcgaaaaa ctcgagcagc tgattcttcc tgacaaggga
     1861 ctctgttccg acattctcag gattgcaaat tcagcctttt acggaagatc aggcaaggtc
     1921 aagactctga aggatgcaat tactctcctt ggaatcagaa caataaagaa tattgcaata
     1981 gtacagacaa agaaaagcat aaataaaaat atttctcatt atcctttatt taaaaaatat
     2041 ctgttcgaac ttcccactct gacagccctt gtttcattcg acctttctgt gccgatggga
     2101 ctgaaaaaaa tccgtgagga gctttttctg cctgcaagct tccgtgattc aggcaagacg
     2161 attcttgcac tgaatctgac caagcagtat catgatgtaa tcgaggtgca tcataagtca
     2221 gggatcagtc tgagtgatct ggaaatggaa gtctttcata caacttcaaa tgaagtagga
     2281 cttcatgtct tcaaggtctg gcatatgccc gaaaatcttt acggaattat tcagaatcag
     2341 agtttcggaa tggatcagat ttcaagtgtg caggatatag acagactgac acgccttgga
     2401 gaaattattt ccagaaagct tctgagtctc tctgtttcct ccggtgaaac agaaatggca
     2461 atgtcaatta tgcagatata caaggtgcat gaggggctca tagatgcctt tgatgaggat
     2521 tattatgaca atataaggac tcatcccttt atggaagttc tctgaaatca gaacagtctt
     2581 ccgaaaaaga aactgttttc tctgataaat ccgtaaagtt tttcaagctc tgtcttatcc
     2641 ggcttctttc cttttctgaa aggggatatg cg
//
LOCUS       DAOQGJ010000353         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24063_length_2662_cov_2.592635, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000353 DAOQGJ010000000
VERSION     DAOQGJ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24063_length_2662_cov_2.592635"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..959
                     /locus_tag="PK683_06805"
     CDS             126..959
                     /locus_tag="PK683_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08194.1"
                     /translation="MTTEPKKLKSAEIRERMGELVQRNYLHFELKGLTKGTRDIISDA
                     VNGIMDRVGANPLNTFHLFSGLMEALLNAVKGNIRYTIFKKEIKARLFDVEPSSQDVE
                     QLLEIILNTSPLRDAIQRYIVPDKIKRDVLNILTWEEKIRVRKLELKTEERVLLQSIR
                     DEIKKDERKISLKIRVDKDMISIRLRNDSPIMDIDMERIRESREKHYHLYLEGKSSDF
                     FRPEFLDEKESAGFGIAMIDEGYYNMKLNPMEYFTYIQGQHATTVYMNYPIEALCSMD
                     F"
     gene            complement(961..1254)
                     /locus_tag="PK683_06810"
     CDS             complement(961..1254)
                     /locus_tag="PK683_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08195.1"
                     /translation="MFVRLFLSFVFFCILASFLIYSENGRDLKGKLFRWAEYGNESMD
                     KCLNKGEEYLTKDEVFELYKSGAYKTERSEDNAYDYENALSYIRYKNKQPCRQ"
     gene            complement(1667..>2662)
                     /locus_tag="PK683_06815"
     CDS             complement(1667..>2662)
                     /locus_tag="PK683_06815"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNH08196.1"
                     /translation="GFLIYGGLSNFYKEHGFIDYKGFFVKRIFRIFPAYYFFLFIILF
                     LGMMQIRLLNSKPNLNFTEMLVLQALKEASLKYDFLYLSNYFGGSVSHNWSLATEEQF
                     YIFLPFFLGFFLLKTKQETRIKSLIIIYFLPLVFRMLHFLLASDTVSESNFIYRAAHT
                     RMDSLFIGILVFEFHSVYRDKFEEIMRYRKYLYGSLIILFFPVFLITHADLPFFADTV
                     KYSLANFFFGIVLLLCLDGQSVFSRAFSFSVFRPVARVSYTAYLWHISVGGAVLGPVS
                     SSGNSFFWFRFSFQVISTVFITLCVGWLLFILVEYPFIRIREKYFSGKDLKSNSA"
BASE COUNT          964 a          489 c          485 g          724 t
ORIGIN      
        1 ctgcttgcca agaatctcat ctaagatatt tttacaggaa acaaaatttg ctgcaatgag
       61 cacaactaga aaaactgctg cagtcatttt tttgagtaca gaaggattcc ggtgaccgaa
      121 attttatgac aactgaacca aagaaactta aaagtgctga aattagagaa aggatggggg
      181 agctcgtaca aagaaactac cttcattttg aactgaaagg acttacgaag ggaactaggg
      241 acatcatatc tgacgcagtg aacggaatta tggacagggt aggagcaaac cccttaaaca
      301 catttcatct tttcagcgga ctcatggaag ctcttttaaa tgctgtcaaa ggaaatatac
      361 gttatacaat ctttaaaaag gaaatcaaag ccaggctttt tgatgtggag ccgtccagtc
      421 aggatgtgga gcagcttctg gaaatcatcc tgaatacaag ccctctcaga gatgctattc
      481 agagatacat cgtcccagac aaaataaaaa gagacgtttt gaacattctg acttgggaag
      541 aaaaaatcag agtcagaaaa ctggaattga aaactgaaga aagagtactt ctgcagtcca
      601 tccgtgatga aatcaaaaaa gatgaaagaa aaatatcgct gaaaatcaga gtggacaagg
      661 atatgatttc catccgtctc agaaatgatt ctcctattat ggatattgat atggagagaa
      721 tccgcgaatc cagggaaaaa cactaccatc tgtacctgga gggaaaatca tctgatttct
      781 tccgtccgga gtttctggat gaaaaagaga gtgccggctt cggaattgca atgatcgatg
      841 aaggttatta taatatgaaa ctgaatccta tggaatattt tacatatata caaggacagc
      901 acgccactac ggtctatatg aattatccca tcgaagcact ttgctcaatg gatttttgat
      961 ttactgcctg cacggttgtt tgtttttata tcgtatataa gacagtgcat tttcatagtc
     1021 atatgcattg tcttcactcc tctccgtttt ataagctcct gatttgtaca attcaaatac
     1081 ctcatcctta gtcagatatt cctctccctt attcagacat ttgtccatac tttcatttcc
     1141 atattcagcc cagcggaaaa gcttaccttt cagatctctg ccgttttctg aatagatcaa
     1201 aaaagaagca agtatgcaga aaaaaacaaa agacagaaac agccttacaa acatttgatt
     1261 atccctttac aaagcctgag atttcagtca gttcatgcac tgttttcaga gtacagggct
     1321 gatgaatcta attgaatcag acgggaaaaa aagaaatata aatacctaat ttttcaatga
     1381 agcataaacc tgcctgaaaa acttggaaaa acttcagttt gcaataatta caatagatac
     1441 ttattataat aatcgattat tatagttttt tagagaagct gaattcattc ctgttctgcg
     1501 aaagcaggaa agaccttgcc tattttaaca gattgtctgt tccgatcaaa tccttatttt
     1561 cggaagcaga actttttgtg cttcctgtgc aaaacagttc tcaaaaaaca gaaaaactga
     1621 caatattatt ttactgcata aaacctattt atgctgacag ctgcatttaa gccgaatttg
     1681 attttagatc ttttccggaa aaatattttt ctctaatcct gataaatgga tattctacca
     1741 gaataaacag gagccatccg acgcataatg taatgaagac tgtcgaaata acctgaaaag
     1801 aaaaccggaa ccagaaaaaa ctgtttccag aactgctgac aggaccaaga actgctcctc
     1861 cgacagaaat gtgccaaagg tatgccgtat agctgactcg tgcaacaggt ctgaatacag
     1921 aaaaactgaa tgccctgctg aaaacggatt gaccatccag gcaaagaagc agaacaattc
     1981 cgaaaaaaaa atttgcaaga ctgtatttta ctgtatctgc aaagaagggc aaatctgcat
     2041 gagtaattaa gaaaacagga aaaaacagaa taatcagaga accataaaga tattttctgt
     2101 atctcattat ttcctcaaac ttatcacgat agacagaatg aaattcaaac acaagtattc
     2161 cgataaagag actgtccatt ctggtatgag ctgctctgta aataaaattg ctttcagaga
     2221 ctgtatcaga agcaagcaga aaatgaagca ttctgaacac aagaggaaga aaataaatta
     2281 taattaagga cttaatgcgg gtttcctgct ttgttttcag aagaaagaat cctagaaaaa
     2341 aaggaagaaa aatatagaac tgttcctcgg ttgcgagaga ccagttatga gaaacgcttc
     2401 ctccgaaata attagataag tataaaaagt catatttcag acttgcttct tttaaagcct
     2461 gaagcacaag catctcagta aaattcagat tcggtttgga attcagcagt ctgatctgca
     2521 tcatccctaa aaaaagaata atgaagagga agaaatagta tgcagggaat attcgaaaaa
     2581 tccttttcac aaagaaacct ttataatcaa taaatccgtg ttccttataa aaattagaaa
     2641 gccccccata gatgagaaat cc
//
LOCUS       DAOQGJ010000354         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24092_length_2660_cov_3.094434, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000354 DAOQGJ010000000
VERSION     DAOQGJ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24092_length_2660_cov_3.094434"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK683_06820"
     CDS             <1..456
                     /locus_tag="PK683_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08197.1"
                     /translation="KPAAEAEKQPDSASKEEGVYRPVSKEECQNIQAKLEKALNTKFQ
                     ITLEKASDFPKGSGCYLETQGNGVHFDMGKTPGAVEKAIAWETDNRYAAGGPEGIQGG
                     LKNGKKVIVYGVNLKEALDAGCSGRPDENCITKPEKQIYTVSAWIGQQD"
     gene            504..1289
                     /locus_tag="PK683_06825"
     CDS             504..1289
                     /locus_tag="PK683_06825"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH08198.1"
                     /translation="MRIWKTFLFTSICFALPVYTQSSKLEENSHLPKSGQTWYAASPK
                     GLNLRSEASDKSEIIAKLPNRAEIKIEKQPGSLVPAEIKFLNVSADENKKSGEMILKG
                     YWVYVTFGTKSGYVFSRFMIPFPPIHKKEYRMGYDISPYLERIFSLDRPLLKKVQMKD
                     GDAKYLKNIYKYTERKGIRLTVEQSENDYGWGNAELFLPGWSLEQAVVFFYSVLIPLE
                     KDPVLNSYSEGQSAEYSLDPIPNTIEFKKEKKGVLVKWHWGAD"
     gene            complement(1513..2199)
                     /locus_tag="PK683_06830"
     CDS             complement(1513..2199)
                     /locus_tag="PK683_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08199.1"
                     /translation="MDNNYTYYFSSGEGKKEISELWINAENSSFIFRHFEDEWNDWPY
                     PHTMEHILSGSAKLPREIVRSGDKDLLKILSENTEFILERMIYRTLTEDDLFYEKSME
                     IFDLQKEDDSAFSRLYKNLCQKEGIAEFPVRPEPAKTPKNFKETYPSSFIIMNVPESK
                     WPKFRIHTGWEDLKISWVRFPRKWSLHSDGTDAVCGGISAEAILRSEERISKSIPKIL
                     SILENGHSSL"
     gene            2465..>2660
                     /locus_tag="PK683_06835"
     CDS             2465..>2660
                     /locus_tag="PK683_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08200.1"
                     /translation="MVKFIFQSNLSFLRWNLFLITFILFQSCVPFGGQSDRDQCLDGD
                     IASSGVKSNTDIACSAYFATK"
BASE COUNT          774 a          522 c          583 g          781 t
ORIGIN      
        1 aaacctgccg ccgaggctga aaaacagcct gattcagcat ctaaagaaga aggtgtttac
       61 aggccggttt caaaagagga atgtcagaac attcaggcaa aacttgaaaa agctttaaac
      121 actaagtttc agataacttt ggaaaaagct tctgattttc ccaaaggcag cgggtgctat
      181 ttggaaaccc aagggaacgg ggttcatttt gatatgggaa aaacacctgg agctgtcgaa
      241 aaagccatcg cttgggaaac agacaacaga tatgcggcgg gagggcctga agggattcaa
      301 ggaggtttaa aaaacggaaa aaaagtcatt gtgtacggtg taaatctgaa agaagcactt
      361 gatgcaggat gttcaggcag acctgatgaa aactgcatta caaaaccgga aaagcagatc
      421 tacactgtca gtgcctggat aggccagcag gactgactcg gataattatt ttacctgctt
      481 cagcagatga atttactggc attatgagaa tctggaaaac ttttttattc accagtatct
      541 gttttgccct tcctgtttac actcagtctt cgaaactgga agaaaacagt catctgccga
      601 agtcaggaca gacttggtac gctgcaagcc caaagggact gaatctcaga tcagaggctt
      661 ctgataaatc ggagatcatt gcaaaactgc cgaacagagc cgagataaaa atagaaaagc
      721 agcctggttc attggttccg gcagaaatca aatttctgaa tgtctctgct gacgagaaca
      781 agaaaagcgg tgagatgatt ttaaaaggat actgggtata tgtgacattc ggcaccaagt
      841 caggttatgt tttcagtcgg ttcatgattc cctttccgcc tattcataaa aaagaataca
      901 ggatgggtta cgatatcagt ccctatctgg aaagaatatt ttccctggac agaccgctct
      961 taaaaaaagt gcagatgaag gatggagatg caaagtattt aaaaaatatc tataaatata
     1021 ccgaaaggaa aggaattcgg ctgacagttg aacagtctga aaatgattac ggctggggaa
     1081 atgcagagct gtttcttccc ggctggagtc tggaacaggc agttgtattc ttttattcag
     1141 ttctgattcc actagagaaa gatcctgtgc tgaattccta ttcggaaggg cagagtgcag
     1201 agtattctct tgatccgatt ccgaatacta ttgaatttaa aaaagaaaag aaaggcgtgc
     1261 tggtaaaatg gcactgggga gcagactgag ggatatttat ctgacaatct caccttgcgc
     1321 aggctggggc gttattggca gaagtctgtc caccgacggt ttcgcaaact ttgaagctgc
     1381 taagggctct gccctcgcgc caatatctga aaccggccgc cagacttatg aagatggcta
     1441 aaaatttgaa tctacgcact ttcttttcga actattggaa atctgggaat gattgatcta
     1501 ttcttaacga ttttataatg atgaatgacc gttttccaaa atggaaagaa ttttcggaat
     1561 acttttagaa atcctttcct ctgatctcaa aattgcctcc gccgaaattc ctccgcatac
     1621 tgcatctgtt ccgtcactgt gcaggctcca tttgcggggg aaccgcaccc aggatatttt
     1681 caaatcctcc caccctgtat gaattctgaa tttaggccat ttcgattccg gcacattcat
     1741 aatgataaaa ctggaaggat atgtctcttt gaaatttttc ggtgtttttg ccggctcagg
     1801 tctgacaggg aattctgcaa ttccttcttt ctggcacagg tttttgtata atctgctgaa
     1861 ggcagagtca tcctctttct gaagatcaaa tatctccatg gatttttcat aaaaaagatc
     1921 atcttctgtc agagtcctgt aaatcattct ttccagaatg aattcagtat tttcggaaag
     1981 tattttcaga agatctttgt ctccggaacg gacgatttct ctgggcagtt ttgctgaacc
     2041 ggaaagaata tgttccatcg tgtgagggta gggccagtca ttccattcat cttcaaaatg
     2101 tctgaatata aaactggagt tctcagcatt tatccagagt tctgaaatct ctttttttcc
     2161 ttcccctgat gaaaaataat acgtatagtt attatccatg ctttttcgga ttcatctcct
     2221 gtctgcagtt ccactgtttt ttcattcgtt gcggttctgt caagtcacaa tgatcttaat
     2281 cagcattttt ctcttcatat atcggcgagg gtgacagcgg tttacatatt tgcgagccct
     2341 caagccgcct gcaggtgttc ctgtcataaa cgtcccctga cacagcatac ggcactctta
     2401 gggatgctca taaaaagctg ctggtaaaat tgtctggaat cattataatg taattgaggg
     2461 taaaatggta aaatttatat ttcagagtaa tctttccttt ttaagatgga atttgtttct
     2521 gattactttt attttatttc agtcctgtgt tccttttgga ggacagtcag acagagatca
     2581 gtgtcttgac ggtgatattg cttcatcggg tgtaaaatca aatactgaca ttgcctgttc
     2641 tgcgtatttt gcgacgaaaa
//
LOCUS       DAOQGJ010000355         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24106_length_2659_cov_3.506528, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000355 DAOQGJ010000000
VERSION     DAOQGJ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24106_length_2659_cov_3.506528"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PK683_06840"
     CDS             complement(<1..382)
                     /locus_tag="PK683_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08201.1"
                     /translation="MIENMNTLEAYETLKKVQFTDEQSREIVRLVARVHETEPASRKD
                     LMESESNVRMEMKNTELRLQKEIEGVRLEIKNTELKLSKEIEGVRLEIKNTELKLSKE
                     IKELDAKLSVKLEKIHSDFIRWLIG"
     gene            610..2583
                     /locus_tag="PK683_06845"
     CDS             610..2583
                     /locus_tag="PK683_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNH08202.1"
                     /translation="MNALYGLLFLLFAFFSNCSFEGKRELVTEIVNLSGEGWFIAFDD
                     NPDFSKPELETKGWQEIEIPGNVRHLDSDHTGKFWLRKNFELDASGIKKNLALTLGKV
                     YERDEVYLNGKLIGINGKRPDDIYQTEYAYNRGRIYPIPANALNAGKNVLAVRISSDI
                     KTYKGIISGENGIGTLYNANDWFLFESVLDLIYVSVFLFIGIFFLINYLKLPEMKEYL
                     AFSFFIIEFSLYELTRNEFRFFVWDNFIFFKLMEYILLYNIPFFYLNFFQRFFMAEPL
                     KHQKIYFLVNALIPVIFVIFRSPALWTQMTSIWSYHLILPLGYTGWVAYNKMKENDRD
                     GYIYGVALFYFVYGVLKEVMIEKAWITADSSIDSALLFFVMTITVALRFRFISLKLSL
                     QKRFEQLREIDRLREKIFQYMNRILTVPLETSIQSVRAMKVDPSSFSKDRILDIGNNY
                     SDMDSALDDIMELSRLEVKTDSPLKDTVPFTDFIKFIIPEGQITYTIKVNPAFEIHNT
                     LDLINSLMVRIIDYEGFKNFTSKDLIITSDLNDQLHFRFMFYHKDPRVTQSLYKQLSD
                     PSIRNISDIRWSIIKETLRLVEGELQMALINKKYLRVDFGLQALPLKKEEAPVKPSAL
                     NLSFNIDFKNIDLKNIKLPDFKNIRFPWKKDEK"
BASE COUNT          754 a          525 c          528 g          852 t
ORIGIN      
        1 caccgatcag ccatcggata aagtcggaat ggattttttc cagtttcacg ctgagttttg
       61 catccagttc cttgatctct ttggaaagtt tcagttctgt atttttaatt tccagtctaa
      121 ccccttcgat ctctttggaa agtttcaatt cagtgttttt gatctccaga cgaacacctt
      181 cgatttcttt ctggagccga agttcggtgt ttttcatttc cattcggaca ttggattctg
      241 attccataag atcttttctg ctggccggtt ctgtttcgtg aaccctggcg acgagcctca
      301 caatttcccg tgactgttca tcggtaaact gcactttttt gagcgtctcg tatgcttcga
      361 gagtattcat attttctatc ataaatatac ctgtaaaaaa acgtcaagaa ttgaattttt
      421 tcttcggatt cttacacttt aagtgcaaaa aaaaaacggg aagatgcaga tgtctgtatc
      481 aatggacagg aaatgattcg gtgttgaatt tgccggcttt ctcaacgctt tccgatccct
      541 gtccctttat ttcccccttt tcataaaaca gtcttgattc tatagacaga atagagagga
      601 tagctctaaa tgaatgcttt gtacggtctt ttattcctgc tttttgcttt tttttccaac
      661 tgttcttttg aaggcaaaag agaactggtg acggaaatcg taaatctttc cggagaggga
      721 tggttcattg catttgacga caatcctgat ttttcaaaac ccgaacttga aacaaagggc
      781 tggcaggaga ttgaaattcc cggaaatgta aggcatctgg acagtgacca taccggaaaa
      841 ttctggcttc gcaagaattt cgaactggat gcgtccggga tcaagaaaaa ccttgcactg
      901 actcttggaa aagtgtatga aagagatgaa gtctatctga acggaaaact gatcgggatc
      961 aacggcaaaa gaccggacga tatttatcag acagaatatg cctacaacag aggcaggatt
     1021 tacccgatcc ctgcaaatgc attgaatgca ggaaagaatg ttctcgctgt tcgaatcagt
     1081 tcagacatta aaacatacaa aggaatcatc agcggagaaa acggtatagg cacgctgtac
     1141 aatgccaatg actggttcct ttttgagtcc gtcctcgatc ttatctacgt gtcagtattt
     1201 cttttcattg gtatattttt cctgataaat tatctcaaac ttcctgaaat gaaggagtat
     1261 cttgcattca gtttttttat tattgaattt tcattgtatg aactgaccag aaatgaattc
     1321 cgtttttttg tctgggacaa ttttatcttt ttcaagctga tggaatatat actgctttat
     1381 aatattcctt ttttctactt gaatttcttt caaaggtttt tcatggcaga acctctgaaa
     1441 catcagaaaa tttattttct ggtaaatgca cttattcctg tgattttcgt catattcaga
     1501 agtcctgctc tatggaccca gatgacttcg atctggtcgt atcatctgat tcttcccctc
     1561 ggctacacag gctgggtggc atacaataaa atgaaggaga atgacaggga cggttatatt
     1621 tacggcgttg cacttttcta ctttgtttac ggcgtgctga aggaagtgat gatagagaag
     1681 gcctggatta cggctgattc ttccattgat tctgcgcttc tgttctttgt catgacaatt
     1741 actgtagcac tcagattcag attcatttca ctgaaactca gtcttcagaa acggtttgaa
     1801 cagcttcggg aaatagacag actccgtgaa aaaatctttc agtacatgaa cagaattctg
     1861 accgttcctc ttgagacaag cattcagtca gtcagagcaa tgaaggtgga cccaagctcc
     1921 ttttccaagg acaggattct tgatataggt aataattatt cggatatgga ttctgccctc
     1981 gatgatatca tggaactgtc cagactggaa gtcaagacag actcgccttt gaaagatact
     2041 gtcccattta ccgatttcat taaattcatt attcctgaag ggcagattac ctatacaatc
     2101 aaagtgaatc cggcatttga aattcacaat acactggatc tgatcaattc acttatggtc
     2161 aggatcatag attatgaagg attcaagaat tttacgagca aggatctcat catcacttcg
     2221 gatttgaatg accagcttca tttccgtttt atgttttacc ataaggatcc cagagtgact
     2281 caaagtcttt acaagcagct ttcagatccg tccatcagga acatcagcga tattcgctgg
     2341 tcgatcatca aggagactct tcgtcttgta gaaggagaac ttcagatggc tctgatcaat
     2401 aagaaatatc tcagagtgga ttttggactg caggcccttc ctctgaaaaa agaggaagct
     2461 ccagtcaagc cttctgctct aaatctgagc tttaatattg attttaaaaa tatcgatctg
     2521 aaaaatatca agctgcctga ttttaagaat atcagatttc catggaaaaa agatgaaaaa
     2581 taaactgata aaactttctg tttacttagg aagcgggata ctttcggttc tgtatggtct
     2641 gagctgtcag tatgcagta
//
LOCUS       DAOQGJ010000356         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24127_length_2658_cov_3.188629, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000356 DAOQGJ010000000
VERSION     DAOQGJ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24127_length_2658_cov_3.188629"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PK683_06850"
     CDS             complement(<1..696)
                     /locus_tag="PK683_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNH08203.1"
                     /translation="MKQPVIIDGARTAFGSFGGSLKDLGSTELGVISSKSALEKSNVK
                     PEEIGEIIFGNVVPSEKNAIYLSRHIGLKTGLPVETPALTVNRLCGSGMEAVILGAKK
                     ILLGEADAVLTGGSESMSQAPYVVRSARWGVKYGPTEFEDMLNQGLTDIYVELPMGMT
                     AENLAMQYNISREDQDEWAAISEERAERATVEGLLKAEIVPVTVGGKNPIVFDKDEFI
                     RGKAAKDKMSGLKP"
     gene            complement(796..1128)
                     /locus_tag="PK683_06855"
     CDS             complement(796..1128)
                     /locus_tag="PK683_06855"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HNH08204.1"
                     /translation="MIKDTISNIEKNIQESTTINEDKKKELLGLVDSLKNEVLNLSET
                     HKEKAKSLANLARETTQAAVQSDKNESQFDHLMDSLSKSVMEFEASHPKLVGVVNSIC
                     TSLSNSGF"
     gene            complement(1424..2206)
                     /locus_tag="PK683_06860"
     CDS             complement(1424..2206)
                     /locus_tag="PK683_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08205.1"
                     /translation="MKILILYILSVLFFQISISAQKTEPSVTYIMPIYKNLPLSLKNP
                     SEYCLEDEKLYTGSEFRAGELNIFSADEKKPLKADFRFAVIVGKLKKDMNSKLKFKSE
                     CPAGYGEMESSQQLRSDWVSKETGFTIGRSSRDIMKEISYIEVQKISEYKGFELRRTK
                     KYGYSSVYFKNSLSTEIPELTITAHYESKGKGKPYPDYRNKTYTNVQKGDKIFFEIPH
                     KYAKEKEDPSDKSVSFHLHSVSATAVTPDIKISLEIIKDYDE"
BASE COUNT          713 a          545 c          511 g          889 t
ORIGIN      
        1 aggtttcagg ccggacattt tatctttggc tgcctttcct ctgatgaatt catccttgtc
       61 gaatacaata gggtttttgc cgccgactgt tacaggaaca atctctgcct tgagaagacc
      121 ttccactgtg gctctctctg ctctttcctc agaaattgca gcccattcat cctggtcttc
      181 acgggatata ttatattgca ttgcaaggtt ttctgcagtc attcccattg gaagttccac
      241 ataaatatca gtcagaccct gattcagcat atcttcaaat tcagtaggtc cgtatttgac
      301 tccccatctc gcacttctga ctacataagg agcctgactc attgattcag agcctccggt
      361 aagaacagca tctgcttctc cgagaaggat tttctttgca ccgaggatca ctgcttccat
      421 tcctgaaccg cagagacggt ttacagtgag agcaggtgtt tctaccggca gaccggtttt
      481 gagcccaata tgacgggaaa gataaatcgc atttttttca gaaggtacaa catttccaaa
      541 aatgatttca ccgatttctt ccggctttac attggacttt tccaaagctg attttgagct
      601 gattactccc agctcagttg atccgagatc tttcaaggaa ccgccgaagc ttccaaaggc
      661 tgttcttgca ccgtcaataa tcacaggttg tttcataata caggtttctg aaagcagaat
      721 gaatttgtcg agttctttat gctgtcagga tttccgataa cacaggctca atgcctctaa
      781 tttgaacttt ggggactaga aaccgctgtt tgaaagagaa gtacaaatcg aattgacgac
      841 gcctaccagt ttaggatgag aggcttcaaa ttccatcaca cttttggaaa gggagtccat
      901 cagatggtcg aactgactct catttttgtc tgactgtact gcagcctgag tggtctccct
      961 ggctaaattt gccaaagact tcgctttttc cttatgagtt tccgatagat tcaaaacctc
     1021 attcttcaga gaatctacaa gcccaagcag ttcttttttc ttgtcttcat tgattgtggt
     1081 agattcctga atgttttttt ctatattgct gattgtgtcc ttaatcattg ttcctctgta
     1141 ctgtaagatt ttatattgtt tttcgcaact gcagtataac ggattctaaa ttattaaatc
     1201 cttatagtta aagaatctgc ttttaaaagg caggtcactt cgggaggatt aaaactgctc
     1261 ctcaacttgg cagccgactt ctaaagctgc ctaagactcc acttttccgg cattttccat
     1321 aaattctctg acaataaatt tcctgccgag cggttacgat ttttctccta catcgggttc
     1381 atcctaaatg ggaacaggtc tttgtcaagc cggattcagt ctgctattca tcgtagtctt
     1441 taataatttc cagtgagatt tttatgtccg gggtgaccgc agttgcagag acggaatgca
     1501 gatgaaaact gaccgattta tcggaaggat cttctttttc ctttgcatat ttgtgaggaa
     1561 tttcaaaaaa gatcttatca cctttctgca cattcgtata tgtcttgttt ctatagtcag
     1621 gataaggttt tccctttcct ttggattcat agtgcgctgt aatggtcagt tcaggaatct
     1681 cagtgctcag agaatttttg aagtatactg aagaatatcc gtattttttc gttcttctaa
     1741 gttcaaatcc tttgtattcg gaaattttct gtacctcaat ataagatatt tctttcataa
     1801 tgtcccttga acttcgccct atagtaaagc ctgtttcctt ggacacccag tcgcttctca
     1861 gctgctgaga actttccatt tctccgtaac ctgcagggca ttctgatttg aatttcagtt
     1921 tagaattcat atcctttttc agttttccga caatgacagc aaaacggaaa tctgccttca
     1981 gaggtttttt ctcatccgca ctgaatatat taagttctcc cgctctgaac tcagaaccgg
     2041 tgtaaagttt ttcatcctcc aggcagtatt cggacgggtt cttcagactc agaggcaaat
     2101 ttttgtatat aggcataata taagtgacag aaggttctgt cttttgagca gagatgctga
     2161 tctgaaagaa aagaacagag agaatgtaca atattagaat tttcataact gcattatttt
     2221 ttcattttag aggaaagcaa gaaatattta gactgctgat ggaaactttt atcagaatgt
     2281 atatgaattt tttcgaataa aatttaaaaa gaactgactg aatccgaaaa tcgggagtct
     2341 aagtgtctct gcagaacaag atgttgagtt tgagcgtgca gtctgattca gaagagagaa
     2401 taattgaaat aatttatagt tattatataa ttataaatgt aatttgtata tttctctgca
     2461 gcccaggtcg ggaatcattg aagaccttcc gaagatttgt ttacggcagc agcttcataa
     2521 acagtaaagt tttcagtact gtcctttgga gaatacactg ttttttccta aggagagctg
     2581 gaagtccttt tatgctgatc accgaaagta attgcgcttt ttacattcga aaatcttgat
     2641 tttgccggct cactaaag
//
LOCUS       DAOQGJ010000357         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24138_length_2657_cov_3.323597, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000357 DAOQGJ010000000
VERSION     DAOQGJ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24138_length_2657_cov_3.323597"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PK683_06865"
     CDS             <1..1232
                     /locus_tag="PK683_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08206.1"
                     /translation="HSYWRTDMGRKVAVTGVGQTRTKLKRKDVNFPELMGEGARLALH
                     DSGLSIDDIDCVVCSSSPEFFEGLSEPEMWATDFTFGALKPHYRIQTGGTVGASTTIA
                     GWYLVASGMFDNVLVVSGDKLSESSVQKGLSMVYSPTMGRDFAAGAPSAVASQTRIYM
                     SKFPNAKEEHFAKIGTMMRKNALNNPNASLKLPQISEEMMMNMAWLSTPFKLLDSCPT
                     SDAACAMVLSCEEKADQMDVPKAWIQTASTISDGVNYVDRDWSDPIALKKAAQSCYEK
                     VGIKNPIEDIDVIEIYDAFTSQHLIWLEGLGLCSRGTAAEALIDSGVTRMDGKLPVNP
                     SGGVLSNNSIGGSAMIRQAEIALQLMGRAGDRQVKNAEIGLAHGWGGAIQFHTVMIMS
                     KEKDIQDSFRKRGNKNG"
     gene            1225..1698
                     /locus_tag="PK683_06870"
     CDS             1225..1698
                     /locus_tag="PK683_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08207.1"
                     /translation="MAENTVKILNGSVEMDYKWSPGQTVGKFLTKLRDEKEIVGVRCP
                     KTSKVFLPPQGWSPYANVKMDRLIPLKTVPRLKAGTVVYEAPWNMPENISLPYMMAAV
                     QFTGADTELIHLVVADEKTLLSLKSGDELEPVWKKEPNGTIRDIEYFVPKKGGTA"
     gene            1695..>2657
                     /locus_tag="PK683_06875"
     CDS             1695..>2657
                     /locus_tag="PK683_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH08208.1"
                     /translation="MSTVYKTECPYSTIGELMQSKADAHPDKNYLIFQEKSWTYREFD
                     EVTNRTSHLLRRNGVKKGDNVAILIPNSPEFLFCYFGTMKCGSTAVTINTLLKSEELE
                     YIINDCEAEVICTTPQYRKMLDGIWGNLPKIRTVILTEPAKEGYPSLILHEEISKSDP
                     KYSEQTEGSDSASIIYTSGTTGHPKGVVLSHSNILYNSYVARQLIDLEENDTALCIMP
                     LFHVNAQIASMMAAMQSSATVVLEEMFKPRTFIQTLKKYRCTTFSGVPTIYNYLNEMK
                     EADGEDLSFLKACVCGAAPMPVEVFHKFEEKFKGKIIEGYGLSEG"
BASE COUNT          807 a          576 c          657 g          617 t
ORIGIN      
        1 cgcattcgta ctggagaact gatatgggaa gaaaagtggc agtgaccgga gtcggtcaga
       61 caagaaccaa actgaaaaga aaggatgtca attttcctga gctgatggga gagggtgccc
      121 gtcttgcact tcacgacagc ggtctttcca tagacgatat tgactgcgtt gtatgcagca
      181 gcagtcctga attttttgaa ggtctgagcg agccggaaat gtgggcgacc gattttacat
      241 tcggagcatt aaaacctcat tacaggatac agacaggagg gacagtagga gcatccacta
      301 ctattgcagg atggtatctg gtggcatccg gaatgttcga caatgtgctg gtggtttcag
      361 gagacaagct gagtgaaagt tccgtgcaga aaggactcag tatggtctac tcgcctacga
      421 tgggacgcga cttcgctgca ggtgcgccgt cagccgtggc gtctcagaca agaatttata
      481 tgagcaagtt tcccaatgcg aaagaagaac attttgcaaa aatcggaaca atgatgagaa
      541 agaatgctct gaacaatccg aatgcatctc tcaagcttcc tcagatctcc gaagagatga
      601 tgatgaatat ggcctggctt tccactcctt tcaaacttct ggattcctgt cctacaagcg
      661 atgcagcctg tgcaatggtt ctttcctgcg aagaaaaggc agatcagatg gatgtgccca
      721 aggcatggat tcagacagcc tccacaattt ctgacggagt aaactatgtg gacagagact
      781 ggtctgaccc gatagctctg aaaaaagctg ctcagagctg ctatgaaaaa gtgggaatta
      841 aaaacccgat cgaagacatt gatgtcattg aaatttatga tgcattcaca agtcagcatc
      901 tgatctggct ggaaggtctt ggactctgct caaggggaac tgctgcagag gctttgattg
      961 attcaggcgt gaccaggatg gacggtaaac ttcctgtgaa tccgagcgga ggagttcttt
     1021 ccaacaattc catcggaggt tctgcaatga tccgtcaggc ggaaattgct cttcagctga
     1081 tgggacgtgc aggagacaga caggtgaaga acgcggagat cggtctcgca cacggatggg
     1141 gcggcgctat acagtttcat actgtaatga ttatgtccaa ggaaaaagat attcaggatt
     1201 cattccgcaa aagaggaaac aaaaatggct gagaacactg taaaaatttt aaacggttca
     1261 gtggagatgg actataaatg gagtccagga cagacagtcg gaaaatttct gaccaagctc
     1321 agagatgaaa aagaaatcgt aggagtcagg tgtcccaaaa cttcaaaagt gttccttccc
     1381 cctcagggct ggtcgcccta tgcgaatgta aagatggaca gactcattcc tctgaagaca
     1441 gttccgagac tcaaggcagg cacagttgta tatgaggctc cctggaatat gccggagaac
     1501 atttctcttc cttatatgat ggcggctgtt cagtttacgg gagcagacac tgaactcatt
     1561 catcttgtgg tcgcagatga gaagactctt ctctcgctga aatccggaga tgaacttgag
     1621 ccggtatgga aaaaggaacc gaacggcaca atacgggata tagaatattt tgttccgaaa
     1681 aaaggaggaa ctgcatgagc acagtgtata aaacagagtg cccctactcc acaatcggag
     1741 aactgatgca gtccaaggca gatgcacatc cggacaagaa ttatctgatc ttccaggaaa
     1801 aatcctggac gtacagagaa tttgacgagg tgacaaacag aacctctcat cttctgcgaa
     1861 gaaacggagt gaaaaaggga gacaatgtcg ccatcctcat tccaaactcg cctgaatttc
     1921 ttttctgcta tttcggaaca atgaaatgcg gaagcactgc agtcacaatc aatactcttc
     1981 taaaatcgga agaactcgaa tacatcatca acgactgcga ggcggaagtc atctgcacca
     2041 ctccgcagta cagaaaaatg ctagacggca tctggggaaa tcttcccaag atcaggacag
     2101 tcattctcac tgaacctgca aaggaaggat atccttctct cattcttcat gaggaaatat
     2161 ccaaatctga cccaaaatat tccgaacaga cagaaggatc agattccgca tccatcatct
     2221 atacctcagg aactacagga catcctaagg gtgttgtact gtctcattcc aatatattat
     2281 acaacagtta tgtggcaaga cagctgattg atctggaaga aaacgatact gcactatgca
     2341 ttatgcctct tttccatgtg aatgcgcaga ttgcatccat gatggctgca atgcagagca
     2401 gcgccactgt ggttctggaa gaaatgttca agcccagaac attcatacag acactgaaaa
     2461 aatacagatg cactactttc agcggagtgc ccacaatcta caattatctg aacgagatga
     2521 aggaggctga cggtgaagac ctgagtttcc tgaaagcctg cgtctgcggc gctgccccga
     2581 tgcctgtgga agtcttccac aagtttgagg aaaaattcaa gggaaagatc attgaaggtt
     2641 acggactttc agaagga
//
LOCUS       DAOQGJ010000358         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24155_length_2656_cov_3.373702, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000358 DAOQGJ010000000
VERSION     DAOQGJ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24155_length_2656_cov_3.373702"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK683_06880"
     CDS             complement(<1..608)
                     /locus_tag="PK683_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNH08209.1"
                     /translation="MNKDITITRGNGKDILLQAFHWNLVKTKGTGTVDGLEKSWYRIL
                     TEKAAQIASDGFTIVYLPPPWVDDSHWEGNGKHGGGEGYFWRDIDLNSRYGTKKELIA
                     LTDAFHKFNIKVVVDIVLNHRDRNRMKTDIWAYPGPQWRAGGEESGGAFMDGSCDLRI
                     EHPDVYSRFKNALSELLTDCKVDGWRWDFVWGYHPSEVMSLI"
     gene            complement(1043..2086)
                     /locus_tag="PK683_06885"
     CDS             complement(1043..2086)
                     /locus_tag="PK683_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08210.1"
                     /translation="MRILLLILLAFALNCTKKMPGDKGDIKPKEPVKGKIAIGEIRYE
                     GKPEEKWLGDFIQNELSDHLHRVFGDQALEEKDFETAYKKAVTAGPKKSLEELNLDAA
                     KNLKADYFLFGKVVNQDPLKVEIKIYKTSDNQSVLNILFDGTKEQFYDLFTFIPEKIN
                     PELSPEQKKLIEVFSTKQYAAIEPYAKANFIEKDADKKIQLLTQSLTADPEYQPALRE
                     LASAYNEKGDSKKALETYIKCREKMEKNQLQNTAAYARTLGNLVEYYSKTNDRKAYIA
                     AFKQYLEAGEKTGIGISQGLFDFGVFLHMKEGKSCEGLVYLEKLAKQPDVKDFELNDF
                     SNLLTDVRELCRK"
BASE COUNT          686 a          602 c          477 g          891 t
ORIGIN      
        1 tttataaggg acataacctc acttggatga taaccccata caaagtccca tctccatccg
       61 tccaccttgc agtcagtcag aagctcagaa agtgcattct tgaatctgga atacacatcg
      121 ggatgttcga tccgcaaatc gcagcttcca tccataaagg ctcctccaga ctcttctcct
      181 cccgccctcc attgaggacc tgggtaagcc cagatatccg ttttcatcct gtttctgtcc
      241 cgatggttca gtacaatatc tacgaccact tttatattga atttatgaaa tgcgtcggtg
      301 agagcgatca gctctttctt tgtgccgtat ctggagttta aatcgatgtc tcgccagaaa
      361 tagccttcgc cgccgccgtg ttttccgttt ccttcccaat gagagtcatc tacccaggga
      421 ggcggaagat aaacaatcgt aaaaccgtcg gaagctatct gggctgcttt ttcagtgagt
      481 atcctgtacc agcttttttc taaaccgtcc actgtacccg ttcccttggt tttcaccagg
      541 ttccaatgaa atgcctgcag aagaatatct tttccgtttc ctctggtaat agtaatgtcc
      601 ttattcatag ggaaggattt taagaatata atcccttaat tcaagaaaaa aataatgcct
      661 tcagtgtttt ttatccggcc cattttcaga cctttttaat caattccata acgtattgat
      721 tttatgctga gtgcacaggt ctccacatct attctttctc aactcgataa aatcgacctt
      781 gcgttcctgc caaaaatgcc tcaaggctgc agaaacaaga atgttttatt ggactgaatc
      841 ttttttgaat tgtaaatgca tattatcagg tcaatgatat cagatgggac cggtacccgt
      901 cggttccatc tgatttaaag agatacagca tagagctgaa aagagttttt tctgaacgct
      961 gcattttaac agcctcaaac ttgaactgaa aatgttcagt ctgcaggttc ttcacagtaa
     1021 agaaagccgc agatcagtaa aactatttcc tgcagagttc ccttacatct gtcagcagat
     1081 tggaaaaatc attcagttca aaatctttta catccggctg cttcgcgagt ttctcaagat
     1141 aaacaagtcc ctcacaggat ttgccttctt tcatatgaag aaaaactcca aaatcaaaaa
     1201 gtccctgaga tattccgatg cctgtctttt ctcctgcttc cagatactgc ttgaatgctg
     1261 cgatgtaggc tttccggtca ttcgttttgg aatagtattc caccagattg ccgagtgttc
     1321 tcgcataggc agctgtattc tggagctgat ttttctccat tttttctctg cattttatat
     1381 aggtttccag tgctttcttg ctgtcgcctt tttcattgta tgcagaggcc agttcacgaa
     1441 gtgcaggctg atactcagga tcggctgtca gagactgcgt cagaagctga attttcttat
     1501 ccgcatcctt ttctatgaaa tttgccttag cataaggttc tatcgcagca tactgctttg
     1561 tgctgaacac ttctatcagc ttcttctgct caggactgag ttcaggattt atcttttcag
     1621 gaatgaaagt aaatagatca tagaactgct cctttgtccc gtcaaaaaga atattcaaaa
     1681 cactttgatt atcagaggtt ttataaatct tgatttcaac tttcagcgga tcctgattga
     1741 caaccttgcc gaaaaggaaa tagtccgctt tcagattctt cgctgcatca aggttcagtt
     1801 cttccaagga tttttttgga ccggctgtga cagctttttt ataagctgtt tcaaaatcct
     1861 tttcttcgag agcctgatca ccgaaaactc tgtgcagatg atcagagagt tcattctgaa
     1921 taaagtcccc cagccatttt tcctcaggct tcccttcata tcgaatctcg ccgattgcta
     1981 ttttgccctt gacaggttcc ttaggcttga tgtccccttt gtccccaggc attttctttg
     2041 tgcagtttaa tgcaaatgca agcagaatca ataacagtat tctcatatat tctcccaata
     2101 tttctcttgg aatggaaatg acgaaagctt actgattcat tcagattttc attggagcag
     2161 acttatttca tccaagcaga caatggacat tcaggcataa gaaagttttt tttcaagtaa
     2221 attacttttg caaaaatgct cacttgtcag tcagtctgaa agcataaagt ttcttattaa
     2281 ttcacctaac gccttcggct gccttcggct gcaaggcggg gcgttttgtg cagagcatac
     2341 cgacagtttc gcaaactttt caactgttat atgggatatt gcacaaaagg aaatcagtaa
     2401 tttttcttat atattagtgt accttaaaaa caaaaaaagg gacttgcgtc cctcttcatc
     2461 aattcccagt tttaagaaaa acttattttg cagatttagc cggctttgat ttcgcagcct
     2521 tagttccctt ggaagcagct tccactgtct tcttaggtgc atttttcttc tcaatatttt
     2581 tctttttatt ctggattttc ttttcttttc tgaatttgat ttcccttttc tttttccttt
     2641 ttaacattac atttcg
//
LOCUS       DAOQGJ010000359         2652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24218_length_2652_cov_3.073546, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000359 DAOQGJ010000000
VERSION     DAOQGJ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24218_length_2652_cov_3.073546"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="PK683_06890"
     CDS             complement(<1..1393)
                     /locus_tag="PK683_06890"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08211.1"
                     /translation="MEQAAVLFLWNSTALRTAMNGSFSKKILVSVTGFILLSVMGFIS
                     SLIFINFQKKDALLVNLAGRQRMLSQKMIKELFLLQIEKNPSRRKSDLDNSVKIFDST
                     LKSLLNGGEAPLDLEWKRSALLPSSSDIELVEQLKKITAIWKEIRPFLDEPEKSLNPE
                     RLAELIEKNNTLLKEMDKAVVIFQNQAENKVSLMFTFQIVFLALGIVLSAFYYFYAGR
                     KIILPLKHISDYMKVIASNKGDLTYKVKIETDDELGMLSINFNQFSEILRLKLLDLFA
                     GFRDIIVNISSVGRRMEESGEKFYRIEKELESGHSELDSILHSITEQNASIAEMNTAA
                     QSLASSSERMKAAVQNIFQMTESGKKDILLVSGRMKDAEKSIFELAEKNFELAGKLHS
                     IDSVMQTVNEIAERTNLLALNASIESARAGERGKGFAVVASEIGKLADASQRAVDEIG
                     KSLKEIIKKISSNS"
     gene            1807..2241
                     /locus_tag="PK683_06895"
     CDS             1807..2241
                     /locus_tag="PK683_06895"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HNH08212.1"
                     /translation="MAKTLPKIEKKAGTSFSPAQAMAAIGLISMAADSHIDPSEVETL
                     IQIVQRIDEFKEYSEKQMQDLLNTITNISQTDGNGVLLGLAADALSTPGLKEAALKLA
                     FLVVAADGNVVKEEEQFLTDLQNVLGISNDRYDEILKQLFGQ"
     gene            2333..>2652
                     /locus_tag="PK683_06900"
     CDS             2333..>2652
                     /locus_tag="PK683_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HNH08213.1"
                     /translation="MARNIPKSAKKTEKPFSPVEAIVAVGLVAIAADGVVEDIETESL
                     LAVLQKIEALKEYSEEQFKTLIDRIVKIGREEGLGTLVNSSAEVLTNQALKEGALMTA
                     VLVVA"
BASE COUNT          828 a          551 c          510 g          763 t
ORIGIN      
        1 aagaattaga actgattttc ttgatgattt ccttcagact tttcccgatt tcatctacag
       61 ctctctgact cgcgtcagcc aattttccaa tttcagatgc cacaacagca aagccttttc
      121 ctctttctcc ggcccttgcc gattctatgg atgcattcag tgcaagcagg tttgtccttt
      181 ctgctatttc attgactgtc tgcatgactg agtcaatgga atgaagcttt cccgcaagtt
      241 cgaagttttt ttctgccagc tcaaaaatac ttttttctgc atccttcatt cttccggaca
      301 ccagcagaat atccttcttt ccggattcag tcatttggaa aatattctgc acagctgcct
      361 tcattctttc cgaagaagaa gcaagagact gagcggcagt attcatttca gcaatggagg
      421 cattctgctc agtgatggaa tgcagaatgg aatccagttc agaatgcccc gattccagct
      481 ccttttcaat ccgatagaat ttttcgcccg actcctccat ccttcgtccg actgagctta
      541 tattgacaat aatgtccctg aatcccgcga aaagatccag aagcttaagt ctgagaattt
      601 cagaaaactg attaaagttt atcgacagca ttcccagctc atcatctgtc tcaattttta
      661 ctttgtatgt aaggtccccc ttgttcgatg caatcacttt catataatca gagatatgtt
      721 ttaatggcag aataattttt ctgccggcat aaaaatagta gaatgcggag agaactattc
      781 caagtgcaag aaatacaatc tggaaagtaa acataagtga aactttattt tctgcctgat
      841 tctgaaaaat gacaacagcc ttgtccattt ccttgagcag ggtgttattt ttctcaatca
      901 gctcggcaag tctttcagga tttaatgact tttccggctc atccagaaaa ggccgaatct
      961 ctttccatat cgcagtaatt tttttaagct gttccacaag ttctatgtct gatgaggaag
     1021 gaagcaaagc agaccttttc cattccagat caagaggagc ttctcctccg tttaaaagcg
     1081 acttcagtgt gctgtcaaaa atcttgaccg aattatccaa atcagatttt cgtctgcttg
     1141 gatttttttc gatctgcaga agaaacagct ccttgatcat tttctgactg agcatcctct
     1201 gcctgccagc cagatttaca agaagagcgt cctttttctg aaaattgata aagatcaagg
     1261 acgatatgaa acccatcact gaaagcagta tgaatcctgt gactgagaca agtatttttt
     1321 ttgaaaagct tccgttcatt gctgtcctga gagctgttga attccataaa aaaagcacag
     1381 ctgcttgttc cataaatctt atacagacag agcagtaaat aaatttatat ttgaatagag
     1441 tctcaatttc cgactgatcc tgaatctcag tgtcgtgaaa cgaatataaa aatttataat
     1501 tataataaaa tataatataa taataactgt atcctgatct gattcggcag aaagaatcag
     1561 cacattaaac aggttcccgt gtcagtactg ttattgctgt ctttcatctt ttcagacccg
     1621 aagaaatcag cacggcctgc agaagaaaat cgattatgca gagtcatcgg agatcattct
     1681 tcctgataca gagcactaaa aaaatgatat ctttctcaaa aaaatgttga ccagttttaa
     1741 tttttcaaat catatagacc gcgttgatat cgtatcagca gaaaagaaga aacccaggag
     1801 acgcttgtgg caaaaacact tcccaaaata gaaaaaaaag caggaacttc cttcagtcct
     1861 gcacaggcaa tggcagcaat tggactgatt tcgatggccg ccgacagcca tatagatcct
     1921 tctgaagtag aaacactgat acagattgtt cagaggatag atgaatttaa agaatattcc
     1981 gaaaagcaga tgcaggatct tctcaataca attaccaata tcagtcagac agacggcaac
     2041 ggtgttctct taggattagc tgccgatgca ctttctactc cgggtctaaa agaggcagca
     2101 ctgaaactgg cgtttctggt cgttgctgca gacggaaatg tagtcaaaga ggaagaacag
     2161 tttcttactg acctgcagaa cgtgcttgga atctccaacg acagatatga tgaaatacta
     2221 aaacagctgt ttgggcagta gacgcatctt caatcacatt ttcacagtca taagtgtatt
     2281 atgaaataaa taatttttca ttaaaaaatt cttgaaagtt caggagtaaa aaatggccag
     2341 aaacattccc aaaagcgcaa aaaaaacaga aaagccgttt agtcctgtcg aagcaattgt
     2401 cgcagtagga ttagtggcaa ttgccgcaga cggtgtcgtg gaagacatag aaacagaatc
     2461 actgcttgcg gttctgcaga aaatagaggc attgaaagag tattcagaag aacagttcaa
     2521 aactctcatt gacagaattg taaaaatcgg aagagaggaa ggactgggaa ccctggtaaa
     2581 ttcctctgca gaggttctca caaatcaggc tttgaaagaa ggtgccttga tgactgcagt
     2641 acttgtagta gc
//
LOCUS       DAOQGJ010000360         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24343_length_2643_cov_2.719088, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000360 DAOQGJ010000000
VERSION     DAOQGJ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24343_length_2643_cov_2.719088"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PK683_06905"
     CDS             <1..684
                     /locus_tag="PK683_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNH08214.1"
                     /translation="EIGPGLGAITHILSRSGKKMSVLEIDPVLIENLKIQNYSVEILE
                     GDALQTISSFREKKVFAAGNLPYSISSELMAAICRELPLCTGGIFMLQREFAERIAKE
                     VSSLSIFLHSFGEWELLKIVSPGCFYPVPDATSALLKYTPYPEKILSPEETVSLEKIL
                     KSFFWGKRKTVSRILQDSPFLNESERKNLGELIDRENSDLIKFRPEDIDKKIYYTLAK
                     KSEFLRMEA"
     gene            686..1159
                     /locus_tag="PK683_06910"
     CDS             686..1159
                     /locus_tag="PK683_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08215.1"
                     /translation="MEIRYFSVLAVFFLVFQSSAFISGERKSGTADPVLVPNLIESSL
                     ETESMQNILQGKRRKPMNPKKKTVSVRILQNQKYTYDLGMFGDEEGASIHKQASHFSV
                     SEIEREPEKAKYYYTPEKDFTGTDEVEIVIERGSDGASPSTDFTVIIIKFTISKK"
     gene            complement(1321..1518)
                     /locus_tag="PK683_06915"
     CDS             complement(1321..1518)
                     /locus_tag="PK683_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08216.1"
                     /translation="MAKKFILDFSAIMGRIKIMEFIDWYFWLSFLFSFTVISYILIHI
                     FVRMTARMVVRNMEKTKNGQV"
     gene            complement(1630..>2643)
                     /locus_tag="PK683_06920"
     CDS             complement(1630..>2643)
                     /locus_tag="PK683_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH08217.1"
                     /translation="KIISECPSLAEKYLPTFWCFNRHLMLIVLMFREYRSKDYEYDLV
                     EELIMNDGGLTGLAWTGIHERHSKNNTPIAVIFHTISGDEQDVKGISRYVRETLHWTA
                     VVCIRRGHGKLPLTSPIINTMGSTSDLKEQLDHIQKKYPEAPLFGIGISAGSGLLARY
                     LGEAGQKSRFTAAAAVSPAYDIEKAFHRIHPAYSKIMGQRIINYFLKKHNDSLSKIPG
                     FEEIQNCRTLGEFQDNLHPIAGFESKEEYYENSNPIKVVKNIRTPLLILNSEDDPICI
                     NENVLENLHWLEKLPHTVLVHTKRGSHVAFFEGIRAEFWSDRVIGEYFQAVLRQSRRK
                     RQG"
BASE COUNT          811 a          543 c          532 g          757 t
ORIGIN      
        1 gagatcggcc ctggactcgg tgcgattact catattctga gcaggtcggg aaagaagatg
       61 tcagttctgg aaattgatcc ggtactgatt gagaatctga aaattcagaa ttacagtgtt
      121 gagattctgg aaggagatgc actgcagaca atcagcagtt tcagagaaaa aaaagtcttt
      181 gcggcaggaa atctgcccta ctcgatcagt tctgaactga tggcggccat ctgcagggaa
      241 ctgcctctct gcacaggagg aatctttatg ctgcagagag aatttgcaga aagaattgca
      301 aaagaagttt cctctctttc gatttttctc cactccttcg gcgaatggga acttttgaaa
      361 attgtaagtc caggatgctt ttatcctgtc cccgatgcaa cttctgcatt attaaaatat
      421 acgccatatc cggaaaaaat tctcagcccc gaagaaactg tttctcttga aaaaattctg
      481 aagtcttttt tctggggaaa aagaaaaact gtttccagga ttcttcagga cagccctttt
      541 ctgaatgaat ctgaaagaaa aaatctgggt gaactcattg atcgggaaaa ctcggatctg
      601 ataaaattca gaccggaaga tattgataaa aaaatctact atactcttgc aaaaaaatct
      661 gaattcctga gaatggaggc ataatatgga aataaggtat ttttctgttc tggcggtatt
      721 tttcctggta tttcagtcgt ctgcatttat cagcggagag cggaaatcag ggactgccga
      781 tcctgtgctg gtgccgaact taatagaaag cagcctggaa acagaatcga tgcagaatat
      841 tctgcagggg aaaaggagaa aacccatgaa tccgaaaaaa aagactgtca gcgtcagaat
      901 cctgcagaat cagaagtata cttatgatct cggaatgttc ggagatgaag aaggtgcaag
      961 catacataaa caggcttctc atttttcggt cagcgaaata gaacgtgaac cggaaaaagc
     1021 caagtattat tatactcccg aaaaggattt taccggaacg gatgaagttg aaatcgtcat
     1081 tgaaagagga tcagacgggg caagtcccag cactgatttc acagttataa tcattaaatt
     1141 tactatatca aagaaataat ctactctttg tattttagaa acagatataa agatagcaat
     1201 gcagcagcgt ttcccaaaga tgcagtcaag gcctttccta taaaatccaa tttctgatac
     1261 tcggacaggc agaagattat aaaaccgaaa gtaagcagga tcagaatcag cacaaaccgt
     1321 tcaaacctgt ccattttttg tcttttccat atttcttaca accattcttg cagtcattcg
     1381 tacaaaaata tgaatcagaa tataagaaat gacagtgaaa gagaataaga aacttagcca
     1441 aaaataccag tcaatgaatt ccataatttt tattcttccc attatggcag aaaaatcaag
     1501 aataaatttc tttgccaaac agcaatcctt tttacatcag aaaggaaaat tgataacccc
     1561 tcctgaaagc aggatttttg cgttttaaga gagtctggag aacggcggcc agcgcataca
     1621 gtgtaatttt cagccctgcc tctttcttct gctctgcctc agcactgcct gaaagtattc
     1681 accgatcact ctgtctgacc agaactcagc tctgattcct tcgaaaaaag ctacatgact
     1741 tcctctttta gtatgtacta atacagtatg aggaagtttt tccagccagt gaagattctc
     1801 cagaacattc tcattgatgc agataggatc atcttcggaa ttgagaatca gaagcggtgt
     1861 cctgatgttt ttgactactt tgataggatt ggaattctca tagtattctt ccttggattc
     1921 aaaaccggca atcggatgaa gattgtcctg aaattccccc agtgtcctgc agttctgaat
     1981 ttcttcaaac ccggggatct tggaaagact gtcattatgt ttttttaaaa aataattaat
     2041 aattctctgt cccattatct tactataagc aggatgaatc cgatgaaatg ccttttcaat
     2101 atcgtatgca ggtgagacag cggctgctgc agtgaatctg cttttctgtc cggcctctcc
     2161 caggtacctt gcaagaagac ccgaccctgc agaaatccct attccgaaaa gaggtgcttc
     2221 aggatatttt ttttgaatat gatccagctg ttccttcaga tccgaagtgg atcccatggt
     2281 attgatgatc ggtgaagtca gaggaagttt tccatgacct ctcctgatgc atacaactgc
     2341 agtccagtgg agtgtctctc tcacatatct ggatattcct ttcacatcct gttcatcccc
     2401 gctgattgta tgaaatatca ctgctatcgg ggtattgttc ttagaatgcc tttcatggat
     2461 tcctgtccag gcaagtccag tcagacctcc gtcattcatt atgagttctt ctacaagatc
     2521 atattcgtaa tccttcgatc tgtattcccg aaacatcagc acaatcagca tcagatgtct
     2581 gttgaaacac cagaaagtcg gtaaatactt ctccgcaagt gaggggcatt ccgaaattat
     2641 ttt
//
LOCUS       DAOQGJ010000361         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24388_length_2639_cov_3.800310, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000361 DAOQGJ010000000
VERSION     DAOQGJ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24388_length_2639_cov_3.800310"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            925..1152
                     /locus_tag="PK683_06925"
     CDS             925..1152
                     /locus_tag="PK683_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08218.1"
                     /translation="MKTTCSICNQEILKLREITQSCPDSETAAARFENALHQKEIKIR
                     DWVEGKYTSEREITELCIDCEIELRSVRFSH"
     gene            1494..>2639
                     /locus_tag="PK683_06930"
     CDS             1494..>2639
                     /locus_tag="PK683_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08219.1"
                     /translation="MGKNQSKLWLFVAGGALILIIAAGILLSNGGKSKGKSGKSGSSD
                     ISGTGDPTQNPGNSGNSGKIGADGLPEDGVTPEFLLAQYKEWAQYPPNSRPITNDHRD
                     IVFPFVIEVPALMMADDPNSKELNNYKCHLQPKTWAVIGPNADMYVKLECKDPTMATI
                     PVQVLDHKVWREKEWDGTRASTVRADFNDDGRDGDETAKDNVITFKWRPTKNDWGNIT
                     IEADIVYAKNKKAKVTTHFFSSPNKPAEVTGTFRESTNEGSLIIHSTVVAYKAGTYHL
                     EANLKEAKEGNFLAYATFDGPLKQGTNEVDFLYFGKILKDKGYDGPYIITDVRGHRVN
                     LPIDPEWANQGEAGLKKIQAAKSTEPDKELVIPFREEYKTKPYMISVF"
BASE COUNT          839 a          538 c          534 g          728 t
ORIGIN      
        1 ccaaaggaat gaattgagtg acaatgccta tccagcccaa ggaaaaaccc agaattacga
       61 aacccagaaa agcagcccac tgttcccata aatgtttatt gtctggattt ttttcggttg
      121 aagtcatatc ttccctcttt tcattttttt gattcttcca ttgaactgca taacccggag
      181 caaaccgatt tttgtcaatc tgtaagaagg gagtgtatat ccgaatccgt tgttccgaca
      241 aatcctcttt ttgtcaaatt gaatgcagaa atttcacttt tctaatttct gcttgcttaa
      301 ttgttcttat catcaaagct ttccatttac atataaaact tatgattatc cgtatctttt
      361 tactaattca ccttacggct gcaggcgggg cggttttttg cagagagcca accgacggtt
      421 tcgcaaacct tgagctgcga agggcttcct tcagaaagta tgctactgat gattggaggt
      481 gtaaatgaca tattgcacag caatcgtatc ctggcttcag aaaatatcat tcctcactgc
      541 cttctaggat ttatattttt tcaatcggca atatcgtacg agctgaatcg attcactgca
      601 atcatcaatt ctccgtcctg atttctgaaa gccgatcgaa agtttcttca caggacaaaa
      661 cctctgttcc agaaacactg tgtcaagctt cctaaaaaca gacttttttt tactgcaatc
      721 ctcagagatc ccaaaattta gaatgggttc acagaaacag gaaaagtcag aagtaatttt
      781 tttttgcgtt tttataaaaa ccgggcagaa aaaaattgac ttttcatcgt ttatgtttta
      841 ttataatatt ttgatttccg tttcgaaaca gagcggccaa attgtatgcg gtatatacag
      901 ccttgaacgg caggataaaa tgagatgaag actacctgca gcatatgcaa ccaagaaatt
      961 cttaaactca gagaaattac tcaaagctgt cctgacagtg aaacagctgc tgcacgcttt
     1021 gaaaatgcac ttcaccagaa agaaatcaag atcagggact gggtggaagg gaaatacact
     1081 tcagaacggg aaataacgga actctgcatt gactgtgaaa ttgaactgag aagtgtcaga
     1141 ttcagtcact gacagattct gattatgaat taaaattaca gtaaaaatat ctctatttca
     1201 ttattcccac tgtacaccgg ccccttggac attcactttc aatcgaccct caggaacggc
     1261 tatgcatgaa ggcactgcga tccaatacag tcaagtttac tggatcatca ctgaacacta
     1321 ttctatcggt ctttatccaa tttttggatt tttattctaa atttatttct tgctttctgc
     1381 agagaaaaat agaacttacg aaagttaatc gtagttcagt acattcggtc agcggggact
     1441 gctcaaaccg attacgctct tgaacagatt cagattaaaa aatagaggtt gctatgggta
     1501 aaaatcagtc gaaactttgg ttgtttgtgg cagggggagc attaatcctg attatcgcag
     1561 caggaatact gctttctaac ggagggaagt caaaaggaaa gtccggaaag tccggttctt
     1621 cagatatttc aggaacagga gatcctacac agaatcccgg gaattctgga aattccggga
     1681 aaatcggtgc agacggactt ccggaagacg gggtcactcc tgaatttctt ctggctcagt
     1741 acaaggaatg ggcacagtat cctcctaact ccagaccaat tacaaatgat caccgcgaca
     1801 ttgttttccc tttcgtgatt gaagttcctg cactgatgat ggcagatgat cccaactcca
     1861 aagaactcaa caattacaaa tgccatctcc agcctaagac ttgggctgta atcggtccaa
     1921 atgcagatat gtatgtaaag ctggaatgca aggacccgac tatggctaca attcctgttc
     1981 aggttctgga tcataaagtc tggagagaaa aagaatggga cggaacaaga gcatccactg
     2041 tgagagcgga tttcaacgat gacggaagag acggtgacga aacagcaaag gacaatgtaa
     2101 ttacttttaa gtggagacct acaaaaaacg actggggcaa cataactata gaagctgata
     2161 tagtttatgc taaaaacaaa aaagcaaaag tgacgactca tttcttctct tcaccgaaca
     2221 aaccggcaga agtcactgga acattccgtg agtccacaaa cgaaggttca ctgatcattc
     2281 attctacagt tgtagcatac aaggcgggca cctatcatct ggaagcaaat ctgaaggaag
     2341 caaaggaagg caactttctc gcttatgcca cttttgacgg accgttgaag cagggaacaa
     2401 atgaagtgga tttcctgtat ttcggtaaaa ttctcaaaga taaaggatat gacggacctt
     2461 atatcattac tgatgtaaga ggacatagag tcaatcttcc tatagatcca gagtgggcaa
     2521 atcagggtga agcgggattg aaaaagattc aagcagcaaa atctactgaa ccggacaagg
     2581 aacttgtcat tcctttccga gaagaatata agacaaaacc ctatatgatt tccgtattt
//
LOCUS       DAOQGJ010000362         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24395_length_2639_cov_2.726393, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000362 DAOQGJ010000000
VERSION     DAOQGJ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24395_length_2639_cov_2.726393"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PK683_06935"
     CDS             <1..464
                     /locus_tag="PK683_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNH08220.1"
                     /translation="VRDVKLASLDEALKELQKLEKAKRVFVPGLWNLNQVLAHCAQSI
                     EYSITGYPKNKSVIIRKTVGKLVLMAFLSKGKMSHNLNDPIPGAPSLPTKEDYFEGLD
                     RLKAAIQKFKEAKEPLAPHFVYDALSKADYDRVHAMHIADHLSAVELRDEY"
     gene            969..1805
                     /locus_tag="PK683_06940"
     CDS             969..1805
                     /locus_tag="PK683_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08221.1"
                     /translation="MRYLVFILVLSSLLFSCRKEKKWDIPIFENPSLEKKNSNVFIKT
                     ISMRGQCYLFRAGVGRKLEEREILQVFDIVGTDRGRIHLQIQQTDFFLGEHSELFIDQ
                     SEGNNSESVIAEISSGKLGIRTDESPARIRIGKNILQTEKGIFTVLRFRDLQIHIWKG
                     AVDFFQENERPIHSDEGSYLSFKGSDWSNRDLSTASNQRILKLISVFKIQESREIYKY
                     LYDMLSLSEQPKEKEKAQEKSKMSEAVNEAEDEEKIDLSRYIPLRKDETFRNNHLKIE
                     GL"
     gene            complement(1888..>2639)
                     /locus_tag="PK683_06945"
     CDS             complement(1888..>2639)
                     /locus_tag="PK683_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08222.1"
                     /translation="LFLFLFTGCFEIIHFLQKNPDQTISVVWNFSISSSLAKGGGNPF
                     GGKEAAEGAKTDSLMDRMKNSEKEITGKFAKFTEDMKFQSYEGEYESGIKMSFKIKDL
                     KGFNKASELDEGFPIAPKYDTVKNQLVFNFLPDKKQREANRAKAESENKDTSSKDAPA
                     KDENAPPMGGEEGMEQIVGQIMSSATYNLILGGGFVPKKAMVIGKNKKDKTQTELEIM
                     KLGDQTSLIRIPFFSLLLKEKEGYDVIITLQ"
BASE COUNT          769 a          484 c          540 g          846 t
ORIGIN      
        1 tggtcagaga cgttaagctt gcttctttag atgaggcact caaggaactt caaaaattag
       61 aaaaagccaa aagagtgttc gtgcctggct tatggaatct gaatcaggtt cttgcccatt
      121 gtgctcaaag cattgagtat tcaattacag gataccccaa aaataagtca gtaataataa
      181 gaaagaccgt tggcaaactt gtattgatgg ctttcctgag taaagggaaa atgagccata
      241 atttgaatga tccaattcca ggagctcctt ctcttccgac taaggaagac tactttgaag
      301 gtttggatcg actcaaggca gcaattcaaa agtttaaaga agctaaagag ccattggcac
      361 cgcattttgt ttatgatgca ttatcgaaag ctgattatga cagagttcat gcaatgcata
      421 tagcggatca tctctctgca gtggagttac gcgacgaata ctgataattc attacctgaa
      481 agttcgaaag tcagatcagg gtaaaaatgt ctgtgtacat ttattttttt cttttctttc
      541 tttatctttc actgatcgga gacggagtga atctgtattt tcagggtaga cattggatat
      601 cattgttact tcctggaatt atcggattgc tttttgtttt tttggtttta tggaagaccg
      661 aatttttgta tatatgcatc acaggcggtt tttttctctt tttttcagat ctgaatttcc
      721 ttgggtcact caactttgaa atatggaaat tttggaaata tattcttatc tcattaaact
      781 caattcttct catttatgct gcagtgaatg cttctcgatt tggaaaattt gaagaaaaat
      841 ctcttttttt caaatttaag ggattgagtt cctaccgggc tgttctttat tttgcaagta
      901 tacttctatt atttggatgg cttggcggaa ttgttctttt cgttccgtag gataacctcc
      961 acatataaat gagatattta gtatttattc tagtattgag ttctttgctg ttttcctgca
     1021 gaaaggagaa aaaatgggat attcctattt tcgagaaccc atcattagag aaaaaaaact
     1081 caaatgtatt tattaaaact atttcgatga gagggcaatg ctatctcttc cgtgcaggag
     1141 tcggcaggaa gctggaagag cgggagatcc tacaggtctt tgatatcgtt ggaacggatc
     1201 gcggaagaat ccaccttcag attcagcaga ctgatttttt tttgggagaa cattctgagc
     1261 tttttataga tcagtcagaa ggaaacaatt cggagtctgt tattgctgaa atatcctcag
     1321 gaaagctggg gataagaacc gacgaaagtc ctgcccgaat caggattggg aaaaatattc
     1381 ttcagacaga aaaaggaatc ttcactgtat tgcgtttccg cgatcttcag atacacattt
     1441 ggaagggggc tgttgacttt ttccaagaaa atgaaagacc gattcattct gatgaaggca
     1501 gttatctttc cttcaaaggt tcggactgga gcaatagaga cttaagcact gcatccaatc
     1561 agcggatact caaactgatc tctgttttca aaattcaaga atctagagaa atttataaat
     1621 atttgtatga tatgctcagt ctctctgaac agccgaaaga aaaggagaaa gctcaggaga
     1681 aatcaaaaat gtctgaggca gtgaatgagg cggaggatga agaaaaaatc gatttgagcc
     1741 ggtatatccc attaagaaag gatgagactt tcaggaacaa ccacttgaaa attgaaggtt
     1801 tataatggga tttgcctaga accgtgtaag aaggcagtgc agtgtgcgca gcccgccaac
     1861 ttctgtcggc ggactgaata ttctcaatta ctgaagtgtg ataattacat catatccttc
     1921 tttctctttc agcagaagtg aaaagaaagg tattctgatc aatgaagtct gatctccaag
     1981 tttcataatt tcaagctcag tctgagtttt gtcttttttg tttttcccta tgaccattgc
     2041 ttttttcgga acaaacccac ctcccaaaat caaattataa gtagcagagg acataatctg
     2101 accgacgatc tgttccatac cttcttcacc gcccatagga ggggcatttt cgtccttggc
     2161 aggagcatct ttggaagagg tgtctttatt ttcagactca gctttcgctc tatttgcttc
     2221 tctctgtttt ttatcaggaa gaaaattgaa tacaagctgg tttttgactg tatcgtattt
     2281 tggtgcgatt ggaaatcctt catcgagttc ggatgcttta ttgaaaccct tcagatcctt
     2341 gatcttaaag ctcattttga ttccagattc gtactcaccc tcataggact gaaatttcat
     2401 atcttctgtg aattttgcaa attttccggt aatctctttt tcagaattct tcattctgtc
     2461 catcaaggaa tcggtttttg caccctcagc agcttccttg cctccgaaag gatttccgcc
     2521 cccctttgcc aggctcgatg aaatcgaaaa attccagacg acactgatgg tctgatccgg
     2581 atttttctgc agaaaatgga taatctcaaa acatccggta aataggaaga gaaaaagga
//
LOCUS       DAOQGJ010000363         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24458_length_2635_cov_3.851163, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000363 DAOQGJ010000000
VERSION     DAOQGJ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24458_length_2635_cov_3.851163"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2635)
                     /locus_tag="PK683_06950"
     CDS             complement(<1..>2635)
                     /locus_tag="PK683_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNH08223.1"
                     /translation="EVKKVNLLPSHYVAIGASAGGLEAIEAFFEQMPPKTNLGFIVIQ
                     HLSPAHKSMMVELLSKRTEMPVYRAEDGMEVHRDSIYLIPPKKNMTIFHGKLFLSDHE
                     ENPRGINLPIDIFFRSAAEDQGEKTVGIILSGTGSDGTRGVRAIKENGGMVMVQGEET
                     AKFDGMPRSAIATGLADFILSPNEMAKQLLLYVKHPFTVKSQRSETLVTEDDVYNKIF
                     SKIREKTKVDFTYYKPSTVVRRIERRMMVNQVNDLREYSRLLDASPVEVTSLYRDLLI
                     GVTSFFRDKDMFEALENRILPEFLENYSGKELRFWAAGCSTGEEAYSIAILFKEVLEK
                     MNRNIDLKIFATDVDQEAIFRAGNGVYPESIVADVSPTLLAKYFTKNDENFVISRRIR
                     EMAVFAHHNIIKDPPFTNIDLISCRNLLIYLQPVLQRKVLEYFNFSLNSGGILILGSS
                     ETTGELTDFFDPVNSKWKMYKSKGKRKVTEISLDANTRITRDYPAVVNPAFPRAVQVS
                     RFQEEKALERLLEASYDYLPLTILVNSAMEIIHIVGNADGYFRVPSGKMVNDIVKMIH
                     PDLSIPIATGLQRVFRKKEEVRYSNVKMEIHHEIINIQIHIRPALEKREQEPLAVIFF
                     IEKSEENKDISSKDHSGYDLGKEAELRIQDLEQELQFTKENLQATVEELETSNEELQA
                     TNEELLSSNEELQSTNEELQSVNEELHTVNSEYQNKIVELTELNYDIQNLLQTSQTAI
                     LFLDENLEVRRFTLKIKEIFNIIETDIGRAFKFISHSLQNVDLVSAAVNAAAKTEVFS
                     KDVKSSDGKWYQLKIFPYQIAQNQFSGVLLHFTEITERIIHESKSNTFRDLFLLAESI
                     GEMGYWEWSPDLDTFSSTDGLM"
BASE COUNT          704 a          588 c          489 g          854 t
ORIGIN      
        1 gcatcaggcc gtccgtgctg ctgaatgtat ccagatcagg actccattcc caatatccca
       61 tctctcctat gctttcagcc agaagaaaaa gatctctgaa tgtattggat ttgctctcat
      121 gaataattct ctctgtgatt tccgtgaaat gaagaagaac tcctgaaaac tggttttgag
      181 caatctgata aggaaatatt ttcagctgat accattttcc gtcagaggat tttacatctt
      241 ttgaaaacac ttctgttttt gccgctgcat tgactgcggc tgatacaagg tctacattct
      301 gaagtgaatg agaaataaac ttgaatgcac ggcctatgtc agtctctata atattgaaaa
      361 tttccttgat tttcagtgtg aaccttctga cctccagatt ctcatcaagg aaaagaatag
      421 ctgtctgtga agtctgaaga agattctgta tgtcataatt cagttcagtc agctctacaa
      481 ttttattctg gtactctgaa tttactgtat gaagctcctc atttacagac tggagttctt
      541 cgtttgtgct ctgaagctcc tcattgctgg acagcagttc ttcattcgtt gcctgaagtt
      601 cctcattgga agtttcaagc tcctcgacag ttgcctgcag attttccttt gtaaactgaa
      661 gttcctgttc aagatcctga attctgagtt cagcttcctt tcctaaatcg taaccggaat
      721 gatccttgga tgaaatatcc ttattttctt cagatttttc aataaaaaat attacagcta
      781 gaggctcctg ttctcttttt tccaaagcag gccggatatg aatctgtata tttataattt
      841 catgatgaat ttccattttc acattactgt atctgacttc ttccttcttt ctgaagactc
      901 tctgaagacc tgttgctata ggaatggaaa gatcagggtg aatcatcttc acgatatcat
      961 tcaccatttt accggacgga actctgaaat aaccgtctgc attgcctaca atatgaatga
     1021 tctccattgc actgtttacc agaatcgtca gcggaaggta gtcataggaa gcctccagaa
     1081 gcctttccag tgctttttct tcctgaaacc tggaaacctg cactgctctg ggaaaagccg
     1141 ggttcacgac tgctgggtaa tctctggtga ttcttgtatt tgcatccaga gaaatttcag
     1201 tcacttttct ttttcccttc gatttataca tcttccactt actgttgaca gggtcaaaga
     1261 aatcagtaag ctctcccgta gtttcactgc tccctaaaat taaaattcct cctgaattga
     1321 gtgaaaaatt gaagtactcc agcaccttcc tctgaagcac aggctgaaga taaataagaa
     1381 ggtttctgca tgaaatcagg tctatatttg taaagggagg atccttaatg atattatgat
     1441 gcgcgaaaac agccatctct ctgattcttc ttgagatcac gaagttctca tcgttctttg
     1501 taaaatactt ggccagaagg gtgggtgaaa catctgcaac aatgctttca ggatacacac
     1561 cattgccggc acggaatatt gcttcctgat ccacatcagt tgcaaatatt ttcagatcaa
     1621 tattcctgtt catcttttcc agaacttcct taaacaggat tgcaattgaa tatgcctctt
     1681 cgccggtaga acagccggcc gcccagaatc gcagctcctt tccggaataa ttttccagaa
     1741 attccggcag aattctgttt tcaagagctt caaacatatc cttgtcgcga aaaaagcttg
     1801 tgactccgat aagcaagtct ctgtaaagac ttgtcacttc tacaggagaa gcatcaagaa
     1861 gtcttgagta ctctctgagg tcatttacct gattgaccat cattcgtctt tcaattcttc
     1921 tgactactgt gctgggctta taataagtaa agtctacttt tgtcttctct ctgattttag
     1981 aaaatatttt attatataca tcatcttctg tcacaagagt ttctgatctc tgcgacttga
     2041 cagtaaaggg gtgttttacg taaaggagaa gctgctttgc catttcattt ggagaaagaa
     2101 taaaatctgc aagtcctgtt gcaatggcac tccgaggcat cccgtcaaat ttggcagttt
     2161 cctctccctg caccataacc attccgccat tttccttgat ggctctgaca cctctagttc
     2221 cgtcgcttcc tgtcccagat aaaatgattc ctacagtttt ttccccctga tcttcagcgg
     2281 ctgaccggaa aaaaatatct atcgggagat tgattcctct gggattttct tcatgatcgc
     2341 tcagaaaaag tttcccgtga aatattgtca tatttttttt aggaggaatc agatagatgc
     2401 tgtctctatg aacttccatt ccgtcctcag ccctgtaaac aggcatttca gtgcgcttgg
     2461 aaagaagttc caccatcata ctcttatgtg caggagaaag atgctgaatg acaataaatc
     2521 ccagatttgt ttttggaggc atctgttcaa aaaatgcttc aatcgcttcc agccctcctg
     2581 ccgatgcacc gatggcaaca tagtgtgagg gaagcagatt tactttcttt acttc
//
LOCUS       DAOQGJ010000364         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24466_length_2635_cov_2.660465, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000364 DAOQGJ010000000
VERSION     DAOQGJ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24466_length_2635_cov_2.660465"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PK683_06955"
     CDS             complement(<1..814)
                     /locus_tag="PK683_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08224.1"
                     /translation="MAIALANPIYDTVFKFLLEDLDIARELISEIIHQEILSIELKPQ
                     ENIVTLDRKTDPKPDSHFFTVYRLDFLAEIRTEEGTAKVLIELQKAKLSTDIMRFRKY
                     LGEQYSKDSIPIITIYFLGYPFDDSLPKVIEVARVFRDRLSNRTLERQKNEFIDSLTH
                     DSFVIQLFGLKTEMRNRLEKILSIFSQAAEKIKEHIVYYDYILDDELQQKIVHRLNYA
                     ASDRNLKRQMDMEEEILMELQDLERKIETQTKTIQESRKAIEEKEKAIKEKDK"
     gene            1097..2092
                     /locus_tag="PK683_06960"
     CDS             1097..2092
                     /locus_tag="PK683_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08225.1"
                     /translation="MKKILVSFLIIFLPMILSAQDAGGGSSNGLVLKGGLFGLGGSFK
                     SELEDKLFITDIIGTNTGGRLNTSWDSTNKLYLLNPLGFDYYKSMGSGSLLLGLEMRG
                     LPFNNLLGFQNNYDYYSIGAFSNNNGLGGLGIMQMTHKFSNQDFRVGYQLTFGQFSVT
                     PMFLLRRFQSSYEGNGLFTNPGVEKYKLNSTTYSGFLGADLLYKFNAQSSVFFNIAFQ
                     SPILGDIMADGNRSYYFVGPNYGVFSLGNGSAQAQIGGNRYQIGYQHNFNEKLFLQVG
                     YHTETLSVKYKGYSDLRISNTTNNDAWGFIIRNVIYGNSTNTEIKSLYLTINYKI"
     gene            complement(2230..2433)
                     /locus_tag="PK683_06965"
     CDS             complement(2230..2433)
                     /locus_tag="PK683_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNH08226.1"
                     /translation="MKNKIKISEDAKQDLKDAIRWYESQEKGVGKELAKEVVEKIEQI
                     GIKPESYSKDSYDVRKSSLKNFP"
     gene            complement(2423..2611)
                     /locus_tag="PK683_06970"
     CDS             complement(2423..2611)
                     /locus_tag="PK683_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08227.1"
                     /translation="MEYIDFLWERYGANKPDLSNSERLELNRRIKKYEDYPETACSLE
                     ELILEMEDELGEKLYLEK"
BASE COUNT          757 a          529 c          483 g          866 t
ORIGIN      
        1 ccttatcctt ttccttgata gctttctcct tctcttcgat ggcctttctg ctttcctgga
       61 ttgtctttgt ctgtgtttcg atttttcttt ccagatcctg cagttccatc agaatttcct
      121 cttccatatc catctgtcgc ttcaaatttc ggtcagaagc agcataattc agtcgatgga
      181 cgattttctg ctgaagttca tcatccaaaa tataatcata atatactata tgttctttga
      241 ttttctccgc agcctgagaa aaaatgctca ggattttttc gagacggttt ctcatttcag
      301 tcttcagacc aaagagctga attacaaaag aatcgtgagt caggctgtca ataaattcat
      361 ttttctgtct ctccaaagta cgattgctca gacggtcacg gaatactctg gcgacttcaa
      421 ttaccttcgg caaggaatcg tcaaatggat atcctaaaaa atatattgta atgataggaa
      481 ttgaatcttt gctgtactgt tctcccagat attttcgaaa acgcataatg tccgtagaaa
      541 gcttcgcctt ctgaagttca atcagaacct ttgcagttcc ttcctcagta cgaatctctg
      601 ccagaaaatc cagacgatag acagtaaaaa aatgagagtc aggttttgga tcagtttttc
      661 tgtccagcgt gactatgttc tcctgaggtt tcagttcaat cgacagaatt tcctgatgaa
      721 tgatttctga aatcagttca cgggcaatgt ctaagtcttc cagaaggaat ttgaatacag
      781 tatcataaat aggatttgca agagcaattg ccattgactg agaataatat aagtactgag
      841 atttgtcaag aagatctcaa agtgtgctga tagggaccaa ccttttccgg ctttttaaaa
      901 gcaatttctt gaaaacggat atttctaact gcaacgcaaa tatggaagcc gccgccgtca
      961 ggcagatctg ttgcaagtac ataagagcag tatcgaaaat tgatactgtc aaaatgtttt
     1021 ttttgccaaa aaacctttgt tttcgaaaaa actgccctaa aaaataatgg aatcagaaga
     1081 aattaaaaga gggcacatga aaaaaattct tgtttctttt ctcatcattt ttctgccaat
     1141 gattctttct gcacaggatg caggcggcgg aagctcaaac ggcttggtac tgaaaggcgg
     1201 acttttcgga cttggaggaa gcttcaaatc cgagctggaa gacaaacttt ttattacaga
     1261 tattattggt acaaacactg gaggcaggct gaacacttca tgggacagta cgaataaact
     1321 gtatcttctg aatcctctcg ggtttgacta ctacaagtca atgggttccg gttcacttct
     1381 tcttggattg gaaatgagag gccttccttt caacaatctt ctcggattcc agaacaatta
     1441 tgattactac tccattggtg cattttccaa caacaacggt ctgggtggtt tgggaattat
     1501 gcaaatgact cataaatttt ccaaccagga tttcagagtc ggatatcagt tgactttcgg
     1561 tcagttttcc gtaacaccta tgttcctatt gagaagattc caatccagct atgaaggaaa
     1621 cggtcttttc acaaatcctg gagtagaaaa atacaaattg aattcgacaa cctattccgg
     1681 gtttttagga gcggatcttc tttataagtt caatgctcaa tcttccgtgt ttttcaacat
     1741 tgcatttcag tcaccgatcc taggcgatat tatggctgat ggaaacagga gttattactt
     1801 tgtaggtcca aactacggtg tattttcgct gggaaatgga agtgctcagg cacagatcgg
     1861 cggaaacaga taccagattg gatatcagca caactttaat gaaaaacttt ttctgcaagt
     1921 cgggtaccat acagaaacac tttctgtgaa gtacaaaggc tacagtgatc tgagaatttc
     1981 gaatactact aataacgatg cttggggatt cattatcaga aatgtgattt acggcaattc
     2041 cacaaacaca gaaatcaagt ctctttatct gactatcaac tacaagatat aagattttgg
     2101 tttggggact gtgtagaagc ggtccctatt ttactttttc cgatctttga aatattcttc
     2161 ctgatttctg cttttgtgcc aaactgcaat gattaagatg aaagggagtt taataatata
     2221 aaatatcagt tagggaaaat ttttaagact ggattttctt acatcataag aatctttaga
     2281 ataagattca ggttttattc ctatttgttc tattttttca acgacttctt ttgcaagttc
     2341 ttttcccact cctttttctt gagattcata ccatcgtatt gcatctttta aatcctgctt
     2401 tgcatcttct gaaattttga tcttattttt caaggtatag cttctctcct aattcatcct
     2461 ccatttcaag aatcaattct tccagactgc aggctgtttc cggataatct tcatattttt
     2521 taattcgcct attcagttcc agcctctcag agttcgacag atcaggttta tttgccccat
     2581 acctttccca caaaaaatca atgtattcca acagctgccg cgatgtatcg tccgg
//
LOCUS       DAOQGJ010000365         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24507_length_2632_cov_3.626698, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000365 DAOQGJ010000000
VERSION     DAOQGJ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24507_length_2632_cov_3.626698"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PK683_06975"
     CDS             <1..590
                     /locus_tag="PK683_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH08228.1"
                     /translation="LQRKNAEEEDEPSVKLSAMYSTRGQTLLSPQENYINTGQGILSL
                     KYPEARAEINISYPLWDEGVKETLREAKVNEKQIEEQEEAFRKEVAVELRNRIDAINT
                     SYLTLKNAKKAKEETDRYFRGLYAKYSSGRFTSLMVKNALDALAQSELQIIQAKVNFN
                     INLVRYDLVKNALFEKYEVDPDKVIEEIIKKTKLQ"
     gene            587..1249
                     /locus_tag="PK683_06980"
     CDS             587..1249
                     /locus_tag="PK683_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08229.1"
                     /translation="MIYYYIAVFIILTMQSVSLRSEDLKTSVSASFWQNMFLLSLEKK
                     ISTEYSIGASLGHFGTVNRFDADFRIGSGYLDYNLRNRQEDYHCFLPFVRIYPFGSGL
                     YASLYAGLLQSERKISGSAELMVSADRTRLTVSSSEVKESYSAEGFSFGYRFVFQSGF
                     FISGEAGILLKQLQKSSRRNRTVSDSQEINSSILLNGYTYPESPARSFERILLLSAGL
                     EF"
     gene            complement(1226..2182)
                     /locus_tag="PK683_06985"
     CDS             complement(1226..2182)
                     /locus_tag="PK683_06985"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNH08230.1"
                     /translation="MNIYEIDRMDELKRLFSSSAVTLGNYDGIHRGHSVLIRHLKEAA
                     SLKSLPSVLITYSPNPAAVLGKNPNHRAIYSERKKEEILQEFGIENLIRIRFTREFSE
                     IHAPDFVRMILKETVHASHIVIGFNHFFGKNREGDYQFLKKISEQSGFVTEEIPPVFY
                     NEEQISSSKIRQCISEGNIKKANCFLGRDFSISGRVVSGDQKGREIGFPTANLSLPEG
                     IIVPGNGVYSGYIKIQNSILPAMTNIGTRPTLNGTELRIESHIIGFQGDLYGTDLEVY
                     LAEKIREEKKFPSFNDLKLQLEEDRKKTEYLISNRSEFKTGT"
     gene            2242..>2632
                     /locus_tag="PK683_06990"
     CDS             2242..>2632
                     /locus_tag="PK683_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08231.1"
                     /translation="MKLFLFFLFTISVYSEKLSINFSHIDEKGRNLKEEEFAGYNELN
                     RWALHQNLKLYPDSSAGGIRDGICRMIPSDGLSFYLESRKEKINRYLYLDLTVYENVS
                     YPELPVRTLNIYVAGRLKSTVTFKYGEA"
BASE COUNT          743 a          538 c          574 g          777 t
ORIGIN      
        1 gactgcagag gaagaatgcg gaagaggaag atgagccttc tgtgaagctt tctgcaatgt
       61 acagcaccag aggacagacc cttctgtctc cgcaggaaaa ctacatcaac acagggcagg
      121 gaatactcag tctgaaatat cctgaggcca gagctgaaat caatatttcg tatcctctct
      181 gggatgaagg agtcaaggag actctgagag aggcaaaggt caatgaaaag cagattgaag
      241 agcaggagga agccttcaga aaggaagtgg cagtcgaact ccgaaacagg attgatgcga
      301 tcaatacaag ttatcttact ctgaaaaatg cgaaaaaggc aaaggaagag acagacagat
      361 atttcagagg actctacgca aagtattcga gcggacgttt cacttctctt atggtgaaga
      421 atgctctcga tgcactggct cagtcggagc ttcagatcat tcaggctaag gtgaatttca
      481 acatcaatct ggtcagatac gatctggtaa aaaatgctct tttcgaaaaa tatgaagtag
      541 atccggacaa ggtcatcgaa gaaatcatca agaaaaccaa gctgcagtga tatattatta
      601 tattgctgtt ttcattattc tcactatgca gtctgtttca ctgcgttcag aagacctgaa
      661 aacttctgta tctgcatcct tctggcagaa tatgtttctt ctcagtctgg aaaaaaagat
      721 cagcactgaa tacagcatag gtgcttctct ggggcatttt ggaacagtaa acagatttga
      781 tgcagatttc agaatcggtt ccggatatct cgattataat ctcaggaaca ggcaggaaga
      841 ctatcactgt tttcttcctt ttgtccgtat ttatcctttc ggttcaggcc tctatgcaag
      901 tctctatgca ggcctgcttc agtccgaaag aaaaatatcg ggatcggcag aactgatggt
      961 ttcggctgac agaacaaggc tgacggtcag cagttctgaa gtaaaggaat cctattctgc
     1021 tgaaggcttt tctttcggtt acagattcgt ttttcagtca ggttttttta tcagtggaga
     1081 agcgggaatt ctgctgaagc agctgcagaa aagttccagg agaaacagaa ctgtttctga
     1141 ttcacaggaa atcaattcaa gcattcttct gaacggatat acatatcctg aaagcccagc
     1201 cagaagcttt gagagaattc ttctgttaag tgccggtctt gaattctgaa cggtttgaaa
     1261 tcagatactc tgtttttttt ctgtcttctt ccagctgaag tttcagatca ttgaaggacg
     1321 ggaatttttt ttcttcacga attttttcag caagatagac ttccagatca gtcccataca
     1381 ggtctccttg gaaaccgatg atatgcgact caattctcag ttccgttccg ttcagtgtcg
     1441 gtctggttcc gatattcgtc attgccggga gtatactgtt ctgaattttt atgtaacctg
     1501 aatacacccc attgcctgga acgatgatgc cttcgggcag tgaaagattt gcagtgggaa
     1561 atccgatttc tcttcctttc tgatcaccgg atacgaccct tcctgaaatg ctgaagtctc
     1621 ttcccagaaa acagtttgcc ttctttatgt ttccttccga gatgcactgc ctgatctttg
     1681 agcttgatat ctgctcttca ttgtaaaata caggaggaat ttcttcagtg acaaatcctg
     1741 actgttcaga aatctttttc agaaactgat agtctccttc ccgatttttt ccgaagaaat
     1801 ggttgaaccc gatgacaata tgagatgcat gtactgtttc cttcagaatc attcttacaa
     1861 aatcaggtgc atgtatctca gaaaactccc tcgtaaatct gatccgaatg agattttcaa
     1921 ttccaaattc ctgcagaatc tcttctttct ttctctctga ataaattgcc ctgtgattcg
     1981 gatttttacc gagcactgct gcaggattcg gagagtatgt gatcagaact gaaggaaggg
     2041 attttaagga tgcagcttct ttcagatgcc gaatcagaac tgagtggcct ctgtgaatgc
     2101 cgtcataatt tcccaacgtg actgcagaac tgctgaacag tcgtttcagt tcatccatcc
     2161 tgtcgatttc gtaaatattc acggctggtc tgattatttt tccacttgct aaatattcca
     2221 gcagaatttc tttagaagtg tatgaaactt ttcctgtttt ttctttttac aatttctgtc
     2281 tattctgaaa agctttcgat caacttcagc catattgatg aaaagggcag gaatctgaaa
     2341 gaagaggagt ttgccggcta caatgaattg aacagatggg cgcttcatca gaacctgaaa
     2401 ctgtatccgg acagcagtgc agggggaatc agggacggga tctgcagaat gattccctcg
     2461 gacggtcttt ctttttacct ggaatcaaga aaggaaaaga tcaacaggta tctgtatctc
     2521 gatctgacag tgtatgaaaa tgtatcctat cctgaacttc ctgtcagaac actgaatatt
     2581 tatgtggcag gcagactgaa atccacagtc acttttaagt atggagaggc tg
//
LOCUS       DAOQGJ010000366         2630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24539_length_2630_cov_3.111068, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000366 DAOQGJ010000000
VERSION     DAOQGJ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24539_length_2630_cov_3.111068"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK683_06995"
     CDS             complement(<1..207)
                     /locus_tag="PK683_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08232.1"
                     /translation="MHKIIVKNLNWVVMREVNAYFNLSDEQYKYYREKGRGHLDWFKR
                     TQIPWIIAELTKIQQAKDPLPSEYL"
     gene            complement(715..2439)
                     /locus_tag="PK683_07000"
     CDS             complement(715..2439)
                     /locus_tag="PK683_07000"
                     /inference="COORDINATES: protein motif:HMM:NF039312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH08233.1"
                     /translation="MKKRLNLLIWVFFGTALSVLYVKSKVVDYQKTERVLEVLRQIKH
                     ADSNLDESLLKTRYYIIKDYDPINQYLSEVQIALNSLDKGENSIRNSGQKMINERLDE
                     FTRIFSEKKEELDRFKGQNAILKNAIHLLPISVEKVKNQKIIKDIVLDSLLKNILLYN
                     LAGEESIKKICEGQLMILKSENRKKTNLELEILTRHSENILLKKKGLDDLVESILSKN
                     TGTALDRLSAAFTIYYNSYLNTANIYRLLLYTFSVLLLFYIAYILVRLLLSSISVKMA
                     NEKLLITNKAYERFVPKESLSILSKNSVIDLKLGDGIRREMSVLFSDIRSFTAMSEKM
                     TPEENFGFINSYLKIMGPIVRRHNGFIDKYIGDAIMALFPGKSEDAVQAGISMLKELK
                     KYNSENRNSAERSPIQIGIGVHIGDMIFGTVGENNRMESTVISDAVNLGSRIEGLTKH
                     YGISLLISENIYEIIHKPDDYKVRHIDRVTVKGKNRPIGIYEVYDADSEELIAKKDET
                     KEALEKAIHLFYSKNPLEAKKILEKASKKFKEDLPIKILLNRCNEWIPKMNDSNADWE
                     TSAGFDFK"
     gene            complement(2439..>2630)
                     /locus_tag="PK683_07005"
     CDS             complement(2439..>2630)
                     /locus_tag="PK683_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNH08234.1"
                     /translation="KVPSLRNVALTAPYLHDGNASELEDAVYIMSRYQLGRPFEKEEI
                     DKVVKFLKTLTGEFKGKKL"
BASE COUNT          705 a          550 c          472 g          903 t
ORIGIN      
        1 caaatattca gacggaagcg gatcttttgc ttgctggatc ttggttaatt ctgcaattat
       61 ccagggaatc tgggttcgtt taaaccagtc tagatggcct cggccttttt cgcgatagta
      121 tttgtattgc tcatcgctga gattaaagta tgcgttcacc tcacgcatca caacccagtt
      181 caagtttttg acaatgatct tgtgcataga gcacgaagcc aagacgagta atgcgaagta
      241 aaccatcaca aatcgcaata tatgtggcaa ttttgagaat tgtttcactt ttataaagta
      301 tccaactgat agggttaggt cgcaaggcaa attgcaaagg cactttcccc attataagag
      361 gacaagtgtc aaatagcgac tatatttcgg ctcattttaa atatcaataa acgagcttca
      421 tctcacaatt tccaaattca cccatagcac agcaacaata aatttcgcga acgcattgta
      481 ttcgatccat tacggctggc cgtgttgacc atcataaagc tggttcggcg gctctgaaac
      541 tgcctaaaat cccgcaagcg tggaagctgc tgtttttact gattcatcgc aagctgtcaa
      601 actgtgagat tttcctaata ttttctgaaa attactgcgt ctagacggta tatcctattt
      661 gcgtaatgtg agtaaatcat taaaaacttc tataactttc attaatgatt cgtattattt
      721 aaagtcaaac ccggcagaag tctcccagtc tgcgtttgaa tcgttcattt ttggaatcca
      781 ttcattgcat ctgttcagga gtattttaat tggaagatct tctttaaact tcttggatgc
      841 tttttccaga attttctttg cctcaagagg gttcttgctg taaaataaat gaatcgcttt
      901 ttccagagcc tcctttgttt catctttctt tgcaatcagt tcctctgagt cggcatcata
      961 tacttcatat attccgattg gacggttttt gccttttaca gtaaccctgt ctatatgacg
     1021 aactttataa tcatccggtt tgtgaatgat ctcatagatg ttttctgaaa ttaaaaggga
     1081 tattccgtaa tgttttgtca gtccctcaat tctggaaccc agattgactg cgtctgagat
     1141 cacagtgctt tccattcggt tgttttcccc tacggttcca aagatcatat ctcctatgtg
     1201 caccccgatt ccaatttgta taggagacct ctcagcactg tttctgtttt cactgttgta
     1261 ttttttgagt tccttcagca tactgattcc tgcctgaacc gcatcttctg attttcctgg
     1321 aaacagggcc atgattgcat caccgatata cttgtcaatg aagccgttat gccttcttac
     1381 aatcggtccc attattttga gataggaatt aatgaatccg aaattttctt ccggagtcat
     1441 tttttccgac attgcagtga atgaacgtat gtctgaaaac aatacagaca tttctctcct
     1501 gattccgtct cccagcttca agtcgatgac tgaatttttg gaaagtatgc tgagggactc
     1561 tttaggcacg aatctttcat atgccttatt tgtaatcagt aatttttcat ttgccatttt
     1621 caccgagatt gaggataaaa gcagtctgac aagaatatat gcaatgtaaa acagcaggag
     1681 tactgaaaat gtataaagga gaagcctgta gatattggca gtgttcagat aggaattata
     1741 atatattgta aaagctgcag acagtctgtc caatgcagtc ccggtatttt tactcagaat
     1801 agattctacc agatcatcca gacctttctt tttgagcaga atgttctctg aatgacgggt
     1861 taaaatttcc agttcaagat tcgtcttctt ccggttttca gattttaaaa tcatcagctg
     1921 accttcgcat attttcttga tactttcttc tcctgcaaga ttgtacagca gaatgttttt
     1981 aagcagcgaa tccagaacaa tatctttgat tattttctga ttttttactt tttcaacgga
     2041 tattgggagc aggtgtattg catttttcag aatggcattc tgtcccttga acctgtccag
     2101 ctcctctttt ttttcactga atattctcgt aaattcgtcc agcctttcat tgatcatctt
     2161 ctgaccggag tttcgtatcg aattttctcc cttgtcaaga ctgttcagtg caatttgaac
     2221 ctcagacaga tactgattta tcggatcata atccttaatg atataatatc tcgtttttaa
     2281 aagcgactca tccagatttg aatcggcgtg ttttatctgc cgaagcactt caaggactct
     2341 ctctgttttc tgataatcaa caactttcga ttttacatac agcacagaaa gagctgttcc
     2401 gaaaaatacc catatgagca ggttcagtct ttttttcatc agagtttttt tcctttaaat
     2461 tcaccagtga gagttttcaa aaactttacg accttatcaa tttcttcttt ttcaaaaggt
     2521 ctgcccagct ggtaccgtga cattatatat acagcatcct ccagctcaga agcattcccg
     2581 tcatgaagat agggtgctgt cagtgcgaca ttcctcaggc tgggaacttt
//
LOCUS       DAOQGJ010000367         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24601_length_2626_cov_3.641385, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000367 DAOQGJ010000000
VERSION     DAOQGJ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24601_length_2626_cov_3.641385"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..929
                     /locus_tag="PK683_07010"
     CDS             96..929
                     /locus_tag="PK683_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08235.1"
                     /translation="MKPLSESFRTYIGKIYPFILFIFPFFSPVSEKAVKTSGIEFVEI
                     PSGEFIFDHSYYYKFQNGLKCPVQDEKCRIKIKITGFSISRNPVSRKQWESVLGNVRF
                     SPWEKMDLPACPECAAAGFSHDDTVDFLKALSLKDTGREDAYRLPTESEMIYLLGSCA
                     GKCGEIRNTGTQFQFNTEVAYKNRFGFTVRPELSDSYIWTDDFASDSYLQDILLAGEN
                     PPYSDPRPKPSARFDYGRTLLSFSFTPDLFQIMRTGYSRSMVPSGKETLMYIVRKGKR
                     K"
     gene            1006..1686
                     /locus_tag="PK683_07015"
     CDS             1006..1686
                     /locus_tag="PK683_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08236.1"
                     /translation="MKRYIIIILCLNFQNCFTLSYEKKFDPSGQKDVIHEFVKDRISA
                     KGSVSAAGSEKEMDAQFPSVLAGEGRMLNMLVNSEQKKAEAYDSFSVRKGNESAVFAK
                     DRDFKLVIGNDSKTVNVNDLELKNEELEDTVHYFAVFTGLYGTVFDIYYVRLKRSEED
                     SGIFIKQFYPKKMRFNGDRISWEQRSRIGYIASNTLFYAGYLLTVPLDIITGIFQVIF
                     YLKSGGVK"
     gene            1754..>2626
                     /locus_tag="PK683_07020"
     CDS             1754..>2626
                     /locus_tag="PK683_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH08237.1"
                     /translation="MIIQHELDNSHITEEERKKNKIRTKSDVILIGAGIMSATLGIFL
                     KELEPDLTITVLERLDNAARESSNAWNNAGTGHSAFCELNYTPLNEDGSVEIKKALNI
                     AASFEVSKEFWASLAEKDIIRPQDFIHSVPHYSFVWGEENVSFLKKRYEALKKEPLFS
                     ELEYTEDRTILKKWLPIVMKGRSAKVPSAGTRMKLGTDVDFGALTRAMFRHLASLEGV
                     SVKYNEEVIDLEREPGGLWNLSAVNQETKEEEHHEANFVFIGAGGGSLPLLEKSDIPE
                     AKGYGGFPVSGQWLR"
BASE COUNT          789 a          488 c          621 g          728 t
ORIGIN      
        1 catctcaaat tccattggac attccgtctg cagtccttaa aacagtttat gcggtctgcc
       61 gaaagactgc tgtatgcaaa gggggtattt ttaaaatgaa accgctgtcc gaatcattta
      121 gaacttatat aggaaaaata tatcctttta tcctgttcat attccctttt ttttctccgg
      181 tttcagagaa ggcagtaaaa acatcaggca ttgaatttgt cgagattcct tccggtgagt
      241 tcatatttga tcattcctat tattataaat ttcagaatgg actgaaatgt cctgtgcagg
      301 atgaaaaatg cagaataaaa attaaaatta caggattttc catcagcaga aatcctgtaa
      361 gcaggaagca gtgggagtct gtgcttggca atgtcagatt ttctccctgg gaaaaaatgg
      421 atctgcctgc ctgtccggaa tgtgccgcag caggattcag tcatgatgac actgtggatt
      481 ttctgaaagc actttcctta aaggacacag gaagggagga cgcttacaga cttcctactg
      541 aatcggaaat gatctatctt ctcggttcct gtgccggaaa atgcggtgag atcagaaaca
      601 ccggaacaca gtttcagttt aatacggaag tggcctataa gaacagattc ggttttactg
      661 tccgacctga actttccgat tcctatatct ggacagatga ctttgcctcc gacagttatc
      721 ttcaggatat tctccttgcg ggcgaaaacc ctccttattc tgatccgaga cccaagccct
      781 ctgcaaggtt tgattacggc aggacgcttc taagtttctc atttactccc gatctgtttc
      841 agattatgag aacagggtat tccagaagca tggtgccttc agggaaggag actctgatgt
      901 atattgtcag gaaaggaaaa agaaaatgaa accgggaaac tcctgttcgg tgaaatacgc
      961 tgaccttttt caatatgcat tttcttatgc agaaggatat gagatatgaa aagatatatt
     1021 ataattattt tatgtctgaa ctttcagaac tgtttcactt taagctatga aaagaaattt
     1081 gatccttccg ggcagaagga tgtcattcac gagtttgtaa aggacagaat ttctgcaaag
     1141 ggaagtgttt ccgcagccgg atcagaaaag gaaatggatg cccagtttcc ttcggtgctt
     1201 gcaggggagg gaagaatgct gaatatgctg gtgaattcag agcagaaaaa agcggaagca
     1261 tatgattcct tttcagtcag aaaagggaat gaatccgccg tttttgcaaa ggacagagac
     1321 tttaagctcg tgataggaaa tgacagcaag actgtaaatg tgaatgatct ggaactgaaa
     1381 aacgaagagc ttgaggatac tgttcattat tttgctgttt ttaccggact ttacggaact
     1441 gtatttgaca tatactatgt caggctgaaa agaagcgagg aggattccgg gatatttatt
     1501 aagcagtttt atcccaaaaa aatgcgtttt aacggagaca ggatttcctg ggagcagagg
     1561 agcagaatcg gatatattgc atccaatact cttttttatg cagggtatct gcttacagtc
     1621 cctctcgata ttattaccgg aatatttcag gtgatcttct atctgaaaag cggcggagtg
     1681 aaatagagga aaaaaattat caataggatt gacggatttc gccttttttg agaatgattc
     1741 tctgaggctt tccgtgatta tacagcatga attggacaat tctcatataa ctgaggaaga
     1801 aagaaaaaaa aataaaatca gaaccaaatc ggatgtcatt ctgatcggtg cggggattat
     1861 gagcgccact ctgggcatat ttctgaaaga actggaacct gatcttacca taactgtact
     1921 cgaaagactg gacaatgcag ccagagaaag ttccaatgcc tggaacaatg ccgggacagg
     1981 tcattctgct ttctgtgaac tgaattacac tcctctgaat gaggacggtt ctgtggaaat
     2041 taaaaaagca ctgaatatag ccgcttcctt tgaagtgtcc aaggaattct gggcgtctct
     2101 tgcggaaaag gatatcatca gacctcagga ttttattcat tcagttcctc attacagttt
     2161 tgtctgggga gaagagaatg tttcctttct gaagaaacgg tatgaagcat tgaaaaaaga
     2221 gcctctgttt tccgaactgg agtatactga ggacagaacg attctgaaaa aatggctccc
     2281 aatagtgatg aagggcagat cagcaaaagt tccttcagca ggaactcgga tgaaacttgg
     2341 aacagatgtg gatttcggcg ctctgacaag ggcaatgttc cggcatcttg cttccctgga
     2401 aggtgtatca gtaaaatata atgaagaagt cattgatctg gaaagagagc ccggtggact
     2461 ctggaatctg agcgctgtga atcaggagac gaaagaagag gagcatcatg aggccaactt
     2521 cgtattcatc ggagcgggag gagggtctct gcctcttctc gaaaagtcgg atattcctga
     2581 agccaaagga tatggcggat ttcctgtaag cggtcagtgg ctccgg
//
LOCUS       DAOQGJ010000368         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24747_length_2616_cov_2.452167, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000368 DAOQGJ010000000
VERSION     DAOQGJ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24747_length_2616_cov_2.452167"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="PK683_07025"
     CDS             complement(<1..2260)
                     /locus_tag="PK683_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH08238.1"
                     /translation="MDSELPAGKLEKAAFEGVMEEVKQGGIAAFSVKNPYLMIVICII
                     TVLMGALAIAKMPKDLLPASKQPAVQILSLYPGMATPNIETNLTYKFERYTGQAAGVV
                     RQESRSIPGVSIVKNFFDENSSSQSQAMASTTALILSVMRRLPPGAQPPIVLPFDPMG
                     SVPLTLVAVGGDFEPSKAYDYAQYYVRREIQSNPGAIAPTQMGGAEKQVIVRLDPSKL
                     KTYNFSPLEIINKLSHQNTFIPAGDVKIGNLDYQVYANGIADTVESLNHFSLRASEGV
                     NVYLKDVGKAEESSIIQTNIVTVDGVEQVYVPVFRQLGANSIEVVERVRESMKELGNS
                     IQGLKLTLVADQTVFIRHAIESIGEEAIVGGGLAALMVFLFLGNPRATFATLLSLPLS
                     TFFVFMGLNLLGQSINIMTLGGMALSIGLLVDNSIVAIENIMRHLKEDENPDHIAVII
                     NGAHEVTMPIIAVTLCNVVVLLPLLMTSGVINVLFGAVAKTVMLAITGSLVSETAVIP
                     LFASRFLSGEIPKLPRFFERIQNSVLRLSGLYGRLLEKVFRRIKLILVCMLVLTVLGL
                     FLSRFIGTELFPRADAGFMVVEVRFESGLRIEETAKRIKNLEKRIREIIPADDLKMII
                     SDIGVYNGYPAAFTGNAGTGDATLTVELTDHRKYSSQHYASVLRREIPKVYPEAELGI
                     QLGGMLTSALNGGAKAPIDIQVEGKNAEESYRTAQELLPEIKKMRGAVDVRIQERFDA
                     PVINVDIDRVKSD"
     gene            2396..>2616
                     /locus_tag="PK683_07030"
     CDS             2396..>2616
                     /locus_tag="PK683_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08239.1"
                     /translation="MILRFIIFLFLFLSVSCSDSLKNPERVSSFSYSLEEMTPNPRIT
                     ECSKIEALKDSPIWKHNREQNTVRLNGQG"
BASE COUNT          743 a          608 c          527 g          738 t
ORIGIN      
        1 catcagactt taccctgtca atgtctacat tgataacagg tgcatcaaat ctctcctgaa
       61 tgcggacatc cacagcaccg cgcattttct ttatttcagg aagaagctcc tgtgctgttc
      121 tataagattc ttctgcattt tttccttcca cctgaatatc gataggagcc tttgctcccc
      181 cgttcagtgc agaggtcagc ataccgccca gctgaattcc cagttctgct tcagggtaca
      241 ctttgggaat ttcccttcga aggacagatg cataatgctg agaactgtat ttccgatgat
      301 ccgtcagttc aacagtcagt gtggcatccc ctgttcctgc atttcctgta aatgcagcag
      361 gataaccgtt atacactcct atgtctgaaa tgatcatttt caaatcatct gcaggaatga
      421 tttcccgaat tctcttttcc agatttttta ttcttttggc tgtctcttca attctgagac
      481 cgctttcaaa acgaacttca actaccataa accctgcatc tgctcttgga aaaagttcag
      541 ttcctataaa acgggaaagg aagagtccca gaacagtcag cacaagcata catacaagga
      601 tgagcttaat ccttctgaat accttttcca gaagacgtcc ataaagccca gatagtctca
      661 gaacagaatt ctggatacgt tcaaaaaatc ttggaagctt tggaatttct cctgaaagaa
      721 atctgctggc aaacagagga atgactgcag tctcagaaac aagagatcct gtgattgcaa
      781 gcattacagt ctttgcaact gctccgaaca gcacattgat gacacccgaa gtcatcagca
      841 gaggaagaag caccacaaca ttacagagag tgactgcaat gatcggcata gtcacttcat
      901 gtgctccatt aatgataaca gcaatatgat ccggattttc gtcctctttc agatgacgca
      961 ttatattttc tatagccaca atggaattgt ccacaagaag tccgatggag agtgccatac
     1021 cgcccagggt cataatattg attgactgcc cgagaagatt gagtcccata aaaacaaaaa
     1081 atgtggacaa gggcagtgag agcagtgtag caaaggtggc ccttggattg cctagaaaca
     1141 gaaataccat cagagctgca agcccacccc cgacaattgc ctcctcaccg atggattcaa
     1201 ttgcatgtct gataaataca gtctgatctg cgacaagagt cagcttgagc ccctgaatgg
     1261 aatttccgag ttccttcata ctttctctta ccctttccac cacttcaatg gaattggctc
     1321 ccagctgacg aaagacagga acatatacct gttcgactcc gtccacagtg acaatattgg
     1381 tctgaatgat gctggattct tctgccttgc ctacatcttt cagatagaca ttgacacctt
     1441 cagatgcacg caaggagaaa tgattcaggc tttccacagt gtctgcaatt ccgtttgcat
     1501 aaacctgata atccagattt ccgattttga catcgcctgc aggaatgaat gtattctgat
     1561 gagaaagttt attgataatt tcaagaggag aaaaattgta agtctttaat ttggaaggat
     1621 ccagtcttac aatgacctgc ttttctgccc cacccatctg agtcggtgca atcgcacccg
     1681 gatttgactg gatttctctt ctgacatagt actgtgcata atcataagcc ttgctgggtt
     1741 caaaatcgcc acccactgcg accaacgtca aaggcactga acccatcgga tcaaacggaa
     1801 gcacgatcgg gggctgagca ccgggcggaa gccttctcat tacagagaga atcagtgcag
     1861 tagtggatgc cattgcctga ctctggctgg atgaattttc atcaaaaaaa ttttttacga
     1921 tactgactcc aggaatggat ctggattcct gtctgacgac tccagcagcc tgtccagtgt
     1981 aacgttcaaa tttataagta agattcgttt cgatattggg agttgccatt ccgggataaa
     2041 gagataaaat ctgtactgcc ggctgctttg atgccggaag cagatccttc ggcatcttgg
     2101 caattgcaag agcacccata agtactgtta taatacagat gactatcatc aggtaaggat
     2161 ttttgacaga gaaggcagca atgcctccct gtttcacctc ttccattact ccctcaaatg
     2221 ctgccttttc cagtttacct gccggaagtt cagaatccat aagtatgctc ctttttttta
     2281 cttctggaca gattctgatg atttttgcgt gatttgtcgt cctggcggat aagacaggac
     2341 ttaaaaaatg aaaaagcttt tcggtttacc gaattttttt atactggaga aacggatgat
     2401 tttgcggttt attatttttc tgtttctttt tctttcagta agctgcagcg attccctgaa
     2461 aaatcctgaa agagtcagct ctttctctta ttcacttgaa gaaatgactc ccaatccccg
     2521 gataactgaa tgcagtaaaa ttgaagcctt aaaagactct cctatatgga agcataatag
     2581 agaacaaaac actgtgcgtc tgaacggaca aggaga
//
LOCUS       DAOQGJ010000369         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24823_length_2611_cov_2.761346, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000369 DAOQGJ010000000
VERSION     DAOQGJ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24823_length_2611_cov_2.761346"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1438)
                     /locus_tag="PK683_07035"
     CDS             complement(179..1438)
                     /locus_tag="PK683_07035"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNH08240.1"
                     /translation="MAPFDVMTLFGKAGGYFVFFLIGIGFGAVLEMSGFGDSRKLAAQ
                     FYFKEMRVLKVMFTAIVVAMVLIFLSSSLGLLDFRAVWINPTYIPSQIVGGLIMGVGF
                     IIGGFCPGTSLVSASTLKLDGVLFITGVFLGVGLFGETVEYFQHFYESGEKERFILSE
                     FFGMSIGVTVTLIVLFALTAFYWAEIGEKIFGENISWEKIQFIPKNTKYIAASILLFS
                     IAASAAFIGQPDAEKKWKNVAYKNESFLSKREVYIHPGELRELMHNRQIYLKLLDVRP
                     EKDFNLFHLRDAERVEMDQLIDTKFADEIIRSGSNTVSVLISNSEKDSTHGWKLLKAN
                     GVLNVYILEGGINKWLEVFPLPETVAEKASEQREEETLKFTFCKTVGEELISSIPEIP
                     HEKDSEKLEYAKKVKLQKKKAAMGGCG"
     gene            complement(1463..1981)
                     /locus_tag="PK683_07040"
     CDS             complement(1463..1981)
                     /locus_tag="PK683_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNH08241.1"
                     /translation="METKEEHYINPYLGGTLLGIVLFLAFYITGNGLGASGAINRIQA
                     GLMKLISADHVDRVGYLAHMAGGNLNPLDHSGIFMLAGTFFGGIISGFFFKRIRFEIR
                     KGPQISNGQRLILALIGGTIMGYGARLARGCTSGQALSGGAVLAVGSWAFMFSVFAGG
                     YAVAYFVRKLWN"
     gene            complement(2081..2548)
                     /locus_tag="PK683_07045"
     CDS             complement(2081..2548)
                     /locus_tag="PK683_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNH08242.1"
                     /translation="MAFILIINAFLSLFYHLASAEIRQFFPPYRTFWDSTFAQIRYYT
                     QGIFKGEEHPLEKTRKRKLNPLQQITYLAILNILLPLQVITGLIIWGQDKFPELLRFA
                     GGITVAAPIHTLCSWLFLTFLMMHIYLTTTGHTWSSSIKGMVLGYEEIKEKNR"
BASE COUNT          731 a          619 c          500 g          761 t
ORIGIN      
        1 attttttcct ttgagttttc cgatatttcc ttaaatgaaa atgattctga ttctgaaacg
       61 ttttgagcgg actgacagcc gataaatagg ataacaagaa cagttctcag cattttttct
      121 ccttgtacag atcttctgat ttatctatcg gcaattcagt attcaggaaa gattttcttc
      181 agccgcatcc tcccatcgca gcttttttct tctggagttt gacttttttt gcatactcca
      241 gcttttcaga atccttttca tgaggaattt ccgggattga ggaaatcagc tcttcaccca
      301 cagtcttgca gaatgtaaac ttcagagttt cttcttctct ctgctccgat gctttttccg
      361 caacagtttc aggaagagga aacacttcga gccatttatt gattccgcct tccagaatat
      421 aaacattcag cactccgttt gctttcagaa gtttccagcc gtgagtggaa tctttttctg
      481 aattggaaat cagaactgaa acagtatttg acccggacct gatgatttca tctgcgaact
      541 tagtatctat cagctgatcc atttccaccc gttccgcgtc tctcagatga aacagattaa
      601 aatccttttc agggcggaca tcgagaagct tgagataaat ctgtctgttg tgcatcagtt
      661 ctctcagttc tcctggatga atataaactt ctctcttgct gagaaaagat tcatttttgt
      721 aagcgacatt cttccatttt ttttctgcat cgggctgtcc tataaatgca gcggaagccg
      781 caatggaaaa aagaagaata gatgcggcaa tatactttgt atttttgggg ataaactgta
      841 ttttttccca ggagatattt tcgccgaaaa tcttctctcc gatctctgcc cagtagaatg
      901 cagtcagtgc aaacagaaca atcagagtga ccgtaactcc tatgctcatt ccgaaaaact
      961 cagagagaat aaacctttcc ttttccccag actcataaaa atgctgaaaa tattccacag
     1021 tctcaccgaa cagtccgact cccagaaata ctccggtaat aaaaagaaca ccgtccagtt
     1081 tcaaagtgga agcagatacc agagatgttc cggggcagaa tcccccgatg atgaatccga
     1141 ctcccataat aagtccgcct acaatctggg aagggatata agtcggattg atccatactg
     1201 ctctgaaatc aagaagcccc agtgaggaac ttaagaagat cagcaccatc gcaactacga
     1261 tagcagtaaa catcactttc aggactctca tctccttgaa atagaactgt gctgcaagtt
     1321 ttctggaatc cccgaatccg gacatttcga gaactgctcc gaatccgatt ccgatcagaa
     1381 agaatacaaa atatcctccg gcttttccga agagtgtcat tacatcaaag ggtgccattt
     1441 tctttctcct ccttcctgct cattaattcc agagctttct gacaaaatat gctacagcat
     1501 atccgcctgc aaaaacggaa aacataaatg cccagctgcc cactgcaaga actgcaccgc
     1561 cgctgagagc ctgtccggat gtgcatcccc gtgcaagcct tgctccgtat cccataattg
     1621 tgccgccgat gagggccagg atcagtctct gcccgttcga aatctgagga cctttcctga
     1681 tttcgaaccg gattcttttg aagaagaatc cggaaataat tccgccgaaa aatgttcctg
     1741 ccagcataaa gattccgctg tgatccagag gattcagatt tccgcctgcc atatgtgcaa
     1801 gatatcccac cctgtccaca tgatctgcgg aaatcagttt catcagacct gcctgaatac
     1861 ggtttattgc acccgatgct ccgagtccgt tccctgtgat gtagaatgca aggaaaagca
     1921 ctatgccgag cagggttccc ccaagataag gatttatata atgttcttct tttgtttcca
     1981 tattattctc cttcacttat tttttttgct gagtaactca ccttgtgcct tctgctgcaa
     2041 atggagcgtt tctgcacagt cccaaatccc tgcattcata ttatctgttc ttttccttaa
     2101 tctcttcata accaagtacc attcctttta tgctggaact ccaagtatgt cccgtagttg
     2161 taagatagat gtgcatcatc agaaaggtca gaaacagcca ggaacacaga gtatgaatcg
     2221 gagctgcgac tgtaattccg cctgcaaatc tcagcagttc agggaattta tcctgtcccc
     2281 agataatcag tcctgtgatg acctgaagag gcaggagtat gttcaggata gccagatagg
     2341 tgatctgctg aagaggattg agcttccttt tcctggtctt ttccagagga tgctcctctc
     2401 ctttgaatat tccctgggta tagtacctga tctgtgcaaa tgtgctgtcc cagaatgttc
     2461 tgtagggcgg aaagaactgc ctgatctccg cgcttgccag atgataaaaa agagacagaa
     2521 atgcatttat aattaaaata aatgccaaaa tattattata actattaact aattaaatca
     2581 aaacaattat atggaacaaa tgaagacagg a
//
LOCUS       DAOQGJ010000370         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24831_length_2610_cov_4.747554, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000370 DAOQGJ010000000
VERSION     DAOQGJ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24831_length_2610_cov_4.747554"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PK683_07050"
     CDS             complement(<1..531)
                     /locus_tag="PK683_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08243.1"
                     /translation="MKIKRYTIYDASVSTLDWLNGRILDWACAGTAYTLDGKKEQLQR
                     YHFAYSFDSAVSSDDGLYVLLYKKLGTKALLLKNGEDFRELDRSYYQSEVYEYPAVFF
                     NDKNGRVCIAHCPKFYNRLEFEDAETGKILTKSDNRELQDIFHSRLAVSPDGKFLLSR
                     GWVWHPFDVIEVFDIEQ"
     gene            914..1291
                     /locus_tag="PK683_07055"
     CDS             914..1291
                     /locus_tag="PK683_07055"
                     /inference="COORDINATES: protein motif:HMM:NF026926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm10 family immunity protein"
                     /protein_id="HNH08244.1"
                     /translation="MDFHFTASGVYADDDGTVLMLVLNSDDEEHYFSVQQSLEYDEQS
                     IKAGMNTYYIERDDQGFGGYGGLKEILLSRRELKAVLNETGKKNLKMDTVTVSLNISD
                     NSFFELKNMLERIFSATGLLKQE"
     gene            1459..2397
                     /locus_tag="PK683_07060"
     CDS             1459..2397
                     /locus_tag="PK683_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002082875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNH08245.1"
                     /translation="MMVKLRISALILISFLSCSLFNSGKDNSDKNKQIALAALALSST
                     GSTSSCSSSTTTGTTVSNSTASLDSSSGCVTGVTTCMDSALPTWIKNNFKCAVGYVSG
                     SNYVFKSQNVPNTKSSYYGTSSPLYEARTTTGTNPNSISTQKMVYTIPVTPTKGSGTD
                     STQGGLVAIGITVNGLAIFNNAAAPPDVLATEAATFDNFMGHPQNSGVYHHHAGAPKI
                     CGDNNTTATNSSTCNNSSLIGISLDGYAIYGQKCKTSSGASETTPTLDSLHGHTSTTE
                     HFSTATYHYHYAYDSTATIYTLMGSYFYGNKGSVSN"
     gene            2369..>2610
                     /locus_tag="PK683_07065"
     CDS             2369..>2610
                     /locus_tag="PK683_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08246.1"
                     /translation="METKVPSAINLTQKLLFLTALIFLSCRETITESSDPLFAMSEGK
                     LFYNGKAFTGKMRSKNPVLEEEYVTSYKNGLEDGEYT"
BASE COUNT          806 a          609 c          488 g          707 t
ORIGIN      
        1 ctgctctatg tcaaaaactt caatcacatc aaaaggatgc catacccatc ctctgctgag
       61 aagaaatttt ccgtccggac tcactgccag tctggaatga aagatatcct gaagttctct
      121 gttgtccgat ttagtcagaa tctttccggt ttcagcatct tcaaattcca gtctattgta
      181 aaatttcggg cagtgggcaa tacagactct tccgttttta tcattaaaaa aaactgcagg
      241 atattcatac acctcagact gataataact tctgtccagt tcacggaaat cctctccatt
      301 cttgagaagc agagccttgg ttccgagttt tttatataag agaacatata aaccgtcatc
      361 tgagctgact gccgaatcaa aactgtaggc aaaatgatat ctctgaagct gttctttttt
      421 cccgtccagg gtataagcgg ttccagcaca agcccagtcc agaatacggc cgtttaacca
      481 gtccagagta ctcaccgaag catcatatat cgtatatctt tttattttca ttgcagcctg
      541 cctagtgcca gattcagaac agactccagt ttactgctct cattaataca gtcaaatacc
      601 tttccaaact gtatcattca gtgaatgata actcacttta tttctctgag tccaagggca
      661 cctttaacac accctgcctg cgtcatacaa gcctttgatt cattgagctc tgaattgcag
      721 attccagaca ttggctgctt tttacgcgaa ttaaataaag atgcaggttc cctggatcag
      781 ccttggattc aaatctcaaa ctgaatcagc tgaaggtgcc ggagcttttt tcctcattga
      841 aaaatgtccc attgaaataa ttggatacag caattcctgt ctctgaaaaa aagtaaataa
      901 caggagaaat caaatggatt ttcattttac agcctcagga gtatatgcag atgacgacgg
      961 aacagttctg atgcttgtac tgaacagtga cgatgaagag cactattttt ctgtccagca
     1021 gtcgctcgag tatgatgaac agagcatcaa agccggaatg aatacttatt atattgaaag
     1081 agatgatcaa ggattcgggg gatatggcgg actcaaagag atcctgcttt ccagaaggga
     1141 gctgaaagct gttctgaatg aaacagggaa gaaaaacctg aaaatggata cagtcaccgt
     1201 ctcactgaat atttccgaca actcattttt tgaactgaaa aatatgctcg agcgaatttt
     1261 ttctgcgaca ggacttttga aacaggaatg atttaaaaca gcccacgttt cattcaagca
     1321 aaattcgtac catcctgact taagggtctt agtaagatta ctatgccaat ctcactgaat
     1381 tccgtgcaaa aggattgctt ttctcagaga gactgaatac tagtctcgaa atcaagcctt
     1441 ataaggagaa tcatactcat gatggtcaaa ctcagaattt cagccctcat tctcatttct
     1501 ttcctcagct gcagtctatt taattccggc aaggacaact cagataaaaa caaacagatc
     1561 gcattggcag cacttgcctt gtcaagcaca ggctcaacct cttcctgctc ttcatccaca
     1621 acaacaggaa ctactgtttc caacagtact gcatctctgg attcaagttc aggatgtgtg
     1681 acaggagtga ctacatgcat ggacagtgct cttccaacct ggattaaaaa caatttcaaa
     1741 tgtgctgtag gatatgtatc cggttcaaat tatgttttca agtctcagaa tgtgcctaac
     1801 actaaaagtt cctattacgg aacctcttct cctctctatg aagcaaggac cacgactgga
     1861 acaaatccaa actcaatcag cactcagaag atggtttata caatccctgt cactcctaca
     1921 aaaggatcag gaacagacag cactcaaggg ggtctggtag ccataggaat cacagtgaat
     1981 ggacttgcca tattcaataa tgcagcggca ccgcctgatg tgcttgcaac agaagctgct
     2041 acattcgaca actttatggg acaccctcag aattccggag tataccatca tcatgcaggg
     2101 gcacctaaaa tctgcggtga caacaacaca actgctacga attcttccac ctgcaacaac
     2161 tcttcactga taggcatctc tctcgacgga tatgcaatct acggacagaa atgcaagact
     2221 tcctccggtg cctctgaaac tacaccgacc ctggactcac ttcatggtca cacgtcaaca
     2281 acagaacatt tcagcacggc aacatatcac tatcattatg catacgattc aactgcaacc
     2341 atttacactc tgatgggatc ttacttctat ggaaacaaag gttccgtcag caattaatct
     2401 gactcagaaa cttctcttcc tgacagctct tatcttcctg agctgtcggg aaacaataac
     2461 ggaaagcagc gaccctcttt ttgcaatgtc agaaggaaaa ttattttata atggaaaagc
     2521 attcaccggc aaaatgcgga gcaaaaatcc tgttctggaa gaagagtatg tcacttccta
     2581 taaaaacgga ttagaggacg gcgaatatac
//
LOCUS       DAOQGJ010000371         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_24875_length_2608_cov_2.778300, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000371 DAOQGJ010000000
VERSION     DAOQGJ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24875_length_2608_cov_2.778300"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="PK683_07070"
     CDS             <1..1713
                     /locus_tag="PK683_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08247.1"
                     /translation="MVKKITTVEFDPSNQNKSETVSAILKRNTVFARPPSLDAGSSQR
                     YVNSCNEASVAAIKKGIAANRIPWKGDKFRDYLFGKINENKLSDTYASADIGTLFNCP
                     YDTNKTLKDSTVNLHLKPILKKLVDDKVLLFFRNEAAPKSSNKSIFLYNTKEEIFERI
                     EIYITYLKRYVIPGFQKIGVISEVGDEELRNIGKLTSVLLGYMDDTYGDQKVVVEELS
                     ILSGYYDAYKDEQAKKELRDKVQEVVKLLESQARLTDVATIRLDGQPFPKELIPQLYS
                     HESVLHTEYNDGNRLFDFILHKSSIPIALENAKKLYEVSENDSELRILARMNVANYVD
                     NAKRQEFISAETASLFRYLPFFTRMWRMLLGNIYVTQREAELIRAQKEAEQKKRIAEA
                     KAKDIAKEKARIVDERMRGQGKENPITSTAKALLGETPATETQVTALDFEAEKRVKEV
                     QKAVISILDTAWDQNLRPDREYLVKELGGEIAEEELLSMLKKHFSKEILSFQVKSVSG
                     GGTRHKWPILISKSYVKRKGKALLEKAMKITDEERSTVTPDQDRFDFWNSCEEFLNRV
                     IGKI"
     gene            1715..>2608
                     /gene="pyk"
                     /locus_tag="PK683_07075"
     CDS             1715..>2608
                     /gene="pyk"
                     /locus_tag="PK683_07075"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNH08248.1"
                     /translation="MHITPDKFRKTKIICTIGPATANSEMIQKLAEAGMNIARMNMSH
                     GDHKFHGGIIQKIRNLNKKLKFPIATLLDTQGPEIRTGEVHTELDLKVGEIFSFHVIP
                     GMGEVEAKSVFVNYTDIINDLKIGDKVTVDNGLINLVVLEKKEKELICRVLDGGKLGS
                     RKHINLPGIRVNLPSITQKDTKDILFGLENDIDFIALSFVRSADDVLALKKLIEEHDG
                     HAKVIAKIEDQEAVRNYKHIIEVSDGIMIARGDLGVEVEIEELPILQRKIIKECASQG
                     KPVIVATHLLESMITAPSPTRA"
BASE COUNT          846 a          510 c          624 g          628 t
ORIGIN      
        1 atggtaaaaa aaatcactac agtagagttt gatccgagca atcagaacaa atcagaaaca
       61 gtctccgcca ttctgaaaag aaatacagtc tttgcaagac cgcccagctt ggatgcaggc
      121 tcgtcacaga gatacgtaaa cagctgcaat gaagcaagcg tggctgcgat caagaagggg
      181 atcgctgcaa accgtattcc ctggaagggt gataaattcc gcgattatct gtttggaaag
      241 atcaacgaga acaaactgag tgatacttac gcttctgctg acataggaac tcttttcaac
      301 tgtccgtacg atacgaacaa gactctcaag gattccacag tcaatctgca tctgaagccg
      361 attctgaaaa agctggtgga cgacaaggtc cttctgtttt tcagaaatga ggctgctccg
      421 aaatccagta ataaatccat ttttctttac aatacaaaag aagagatttt tgaaaggatt
      481 gagatttata tcacttatct caagagatat gtcattcctg gttttcagaa gatcggagtc
      541 atttcggaag tcggtgatga ggaactgagg aatattggca agctgacatc tgttcttctg
      601 ggatatatgg atgatacata cggtgatcag aaagttgtag tggaagaact ttccattctg
      661 agcggctatt atgatgccta caaggatgag caggccaaaa aagaactcag agacaaggtt
      721 caggaagttg taaaacttct cgaaagtcag gcgagactca ctgatgtggc tacgatccgg
      781 ctcgacggac agccgtttcc taaggaactt attcctcaat tatattctca tgaaagtgtg
      841 ctccatactg aatacaatga cgggaaccgt ctgtttgact tcatacttca caaatcatca
      901 attccgatag cattggaaaa tgctaaaaaa ctttatgaag tgtcggaaaa tgattctgaa
      961 ctcagaattc tggcaaggat gaatgtggcc aattatgtgg acaatgcaaa aaggcaggaa
     1021 ttcatcagtg cggaaactgc aagtcttttc cgttatcttc cgttcttcac aagaatgtgg
     1081 agaatgcttc tcggcaatat ctatgtcact cagagagagg cagaactgat cagagcccag
     1141 aaagaagcag aacagaagaa aagaattgct gaagcaaaag cgaaggatat cgccaaggaa
     1201 aaagcacgta ttgttgatga aagaatgcgc ggtcagggaa aggagaatcc gattacatcc
     1261 actgcgaagg cacttttggg agaaacccca gctactgaaa ctcaggtgac tgcattggat
     1321 tttgaagcag aaaaaagagt caaggaagtg cagaaggcag tgatctccat cctggacaca
     1381 gcctgggatc agaatctccg ccctgacaga gagtatcttg tgaaggaact cggcggtgag
     1441 atagcagagg aagaacttct ctccatgctc aagaaacatt tttccaagga aattctttcc
     1501 tttcaggtga agtccgtttc aggcggaggc acaaggcaca agtggccgat cctcatttcc
     1561 aaatcatatg taaaaagaaa aggaaaggca cttctagaaa aagcaatgaa aataacagat
     1621 gaagaaagat caacagtcac gccggatcag gacaggttcg acttctggaa ttcctgtgaa
     1681 gagttcctga acagagtcat cggcaaaatt tgatatgcat ataaccccgg acaagttcag
     1741 aaagactaag attatctgta ccatagggcc tgcgactgca aactctgaaa tgattcagaa
     1801 gcttgcggag gcgggaatga atattgccag aatgaatatg tcgcacggcg atcataagtt
     1861 tcacggaggc attattcaga aaatcaggaa tctgaacaag aaactgaaat ttccgattgc
     1921 cacactgctg gatacgcagg gacctgagat tcgaacaggc gaggttcata cagagctgga
     1981 tctgaaagta ggagagattt tttcctttca tgtcatccct ggaatgggcg aagtggaagc
     2041 caagtctgtt tttgtaaatt atactgatat aatcaatgat ctgaagatcg gcgacaaggt
     2101 gactgtggac aacggactca tcaatcttgt agtgcttgaa aaaaaggaaa aagaactgat
     2161 ctgcagggtt ctcgacgggg gaaaactggg aagcagaaag catatcaatc ttcccggcat
     2221 ccgggtcaat cttccttcca tcactcagaa ggatacaaag gacattcttt tcggtctgga
     2281 aaatgacatc gattttattg cactttcctt tgtcagaagt gcagatgatg ttcttgcatt
     2341 gaaaaaactc attgaggagc atgacggtca cgcgaaagtc atcgcaaaga ttgaggatca
     2401 ggaagcagtt cgtaattata aacacattat agaagtgtca gacggcatca tgatcgcaag
     2461 aggtgatctg ggtgtggaag tggaaataga agaacttcct atcctgcaga gaaagatcat
     2521 caaggaatgt gccagtcagg gcaaacctgt gattgtggcg actcatcttc tggagagcat
     2581 gattactgca ccttctccta ccagggct
//
LOCUS       DAOQGJ010000372         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25058_length_2598_cov_2.260716, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000372 DAOQGJ010000000
VERSION     DAOQGJ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25058_length_2598_cov_2.260716"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1679
                     /locus_tag="PK683_07080"
     CDS             159..1679
                     /locus_tag="PK683_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08249.1"
                     /translation="MKVLIADDSPTIRVYLKSILLKEKFEVVEAASGNEAVEKFKKED
                     FDLVTMDVEMPGLNGFEACSFMRNFELKEKKKKSSAVTPVLFITANDTLPGRIKGFES
                     GCADFITKPFSRDEILLSIQNIFNPRNDLAGAGILVVEDSFVTRALIAKILIGQGAEV
                     SEAESGAAALEIIKSSPEKIDIIMTDYFMPGMNGDELCHTLRNRMGIRDIPIIFVTGI
                     AEHEYVLNIYKAGGSDYIIKPFIKEEIIALLNLHVRNLMMSRAQKKQMNEMKKLHLMK
                     DEIMKVLSHDLRTPLNAILGYSEILMNSEEDSVKKKKISYIAQSGSLLLGLVQTLNDI
                     AEAQFSDRMDISEISISKAAENAVSLLLPSAEMKGIRIHFTAEGDNHFLYGNENALLR
                     ILNNLIGNSVKFTESGGEIHVSVSSVGGKSVLTVEDSGTGMPEVFYKEGIQPGRSSLG
                     TAGEKGTGLGLSIVKTLTEKMNGTFEILPAEKKGSRVRVSFPLPDDHSASPSSQAS"
     gene            complement(1646..2494)
                     /locus_tag="PK683_07085"
     CDS             complement(1646..2494)
                     /locus_tag="PK683_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08250.1"
                     /translation="MAFKDEFPEAAEKFGIHILQSDSHPVRSCLGNTDDILNAVEKGI
                     GLDKDGNKTLANPEIPNEKIILLCYSKGMPDALELLSIRPDLRNRITAVFNIAGAPGG
                     SFLANRFYDLIKDLEIRYEFSREFLYLLKLINPLVQIPENFGPVRLNEFDLKGGLLDL
                     TTFRRESFLKEHMDSINDLKIPFFSLPAFTTAMEVPYFQLQGYLEIDKFDTSNDMQVA
                     YRHSVLNCSMNTNLALLHGHHWDVCYGPFPKSMRLGSPNLDHHFPKTASLDACIQLAW
                     ELGLAE"
BASE COUNT          786 a          513 c          594 g          705 t
ORIGIN      
        1 ggacagttcc tgatcttgca gtcaagtccc ttcttttttc gcttcttgcg gggcttacag
       61 ttcctcttcc tttcttcaaa aacctgataa aaaaatggaa agacgaaaat aaactgatcc
      121 gctaaactga tgaaaaccgg tttacggaag gaaagaagat gaaagttctg attgccgatg
      181 acagtcctac tatccgtgtc tacctgaaat cgattctttt gaaggaaaaa tttgaagtag
      241 tggaagctgc ttcggggaat gaggcagtcg agaaatttaa aaaagaagat ttcgacctgg
      301 tcacaatgga tgtggaaatg cccgggctga acggatttga agcatgcagt ttcatgagaa
      361 attttgaact caaggaaaag aagaaaaaat cttctgcggt cactcctgtt cttttcatta
      421 ctgcaaatga tacacttccg ggcagaataa agggttttga gtcaggctgt gcagatttca
      481 taactaaacc tttttcaagg gatgaaatac ttttgtccat tcagaacata ttcaatccaa
      541 gaaacgatct tgccggtgca ggaattctgg ttgtggagga ttcctttgtc acaagggctc
      601 ttattgcaaa gattctgatc ggacagggtg ccgaagtaag tgaagcggaa tcaggggctg
      661 ccgctctgga gatcattaaa agcagtcctg aaaaaattga tattataatg actgattact
      721 ttatgcctgg aatgaacggt gatgaactct gtcatactct tagaaacagg atgggaatcc
      781 gcgacattcc tataatattc gtaactggaa tagccgaaca cgagtatgtg ctgaacattt
      841 acaaggccgg cggatcagac tatattataa aaccttttat aaaagaggag attatagctc
      901 ttttaaatct tcatgtcagg aatctgatga tgagcagggc tcagaagaag cagatgaatg
      961 agatgaaaaa gctccacctg atgaaggatg agattatgaa agtcctttcc cacgatctca
     1021 ggacgccctt gaatgcaata ctgggatact ctgaaatact catgaattca gaagaagact
     1081 ctgtcaagaa gaaaaaaatt tcctatatcg ctcagtcagg cagccttctt ctgggattgg
     1141 ttcagactct caatgatatc gctgaagcgc agttctctga caggatggat atttctgaaa
     1201 tcagcatatc taaagcggcg gaaaatgcag tctcccttct tcttccttca gctgaaatga
     1261 aaggcatcag aatccatttt actgcagaag gagataatca tttcttgtac ggaaatgaaa
     1321 atgcactttt aaggatattg aacaatctga ttggaaattc cgtaaaattt acagagagcg
     1381 ggggagagat ccatgtctcc gtttcctccg tcggcgggaa atctgtactt actgttgaag
     1441 acagcggaac aggaatgccc gaggtttttt ataaggaagg aattcagcca ggcagatctt
     1501 ctcttggtac tgccggtgag aagggcacag gtttaggtct ttctatagta aagacgctta
     1561 cggaaaaaat gaacggtaca tttgagattt tacctgcaga aaaaaaagga agcagagtcc
     1621 gagtcagctt ccctctgcca gatgatcact ctgcaagtcc aagttcccag gcgagctgaa
     1681 tgcaggcatc cagagaagcg gtcttcggga aatgatgatc caaattaggt gaacccagcc
     1741 tcatactttt aggaaaaggt ccataacaca catcccagtg atgtccgtgc agaagtgcaa
     1801 gatttgtatt catactgcag ttcagtacag aatgacggta tgccacctgc atatcattgc
     1861 ttgtatcgaa cttgtcaatt tccagataac cctgaagctg aaaatagggg acctccatcg
     1921 cagtagtaaa tgcaggaagg ctgaaaaagg gaattttcag atcattgatg ctgtccatat
     1981 gttctttcag aaaggactct cttctgaatg tggtcagatc gagcagtcct cctttcagat
     2041 caaattcatt cagtctgact ggtccgaaat tttcaggtat ctgcaccaga gggttgatca
     2101 gcttgagcag gtaaagaaat tcccgactga actcgtatct gatttcgaga tccttgatca
     2161 gatcgtaaaa tctgttagca agaaaggatc ccccgggagc ccccgcaatg ttgaagactg
     2221 cagtgattct gtttcgaaga tcaggacgaa tggaaagaag ttccagagca tccggcatcc
     2281 ccttgctgta acataaaaga atgatttttt cattaggaat ttccggattt gcaagagttt
     2341 tattaccgtc cttgtccaga ccgattcctt tttcgactgc attcagtatg tcgtctgtat
     2401 tgccgagaca gcttctgaca ggatgagagt ccgactgaag aatgtgaatg ccgaactttt
     2461 ctgccgcctc aggaaattca tccttgaatg ccattacagg aagaagcccg ttcagaaatc
     2521 cgggacagaa tatgagcgtt gtattttttt tcgtctaatt aactttaatt gacggatgaa
     2581 taggggaaaa aaccactg
//
LOCUS       DAOQGJ010000373         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25073_length_2597_cov_2.340677, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000373 DAOQGJ010000000
VERSION     DAOQGJ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25073_length_2597_cov_2.340677"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PK683_07090"
     CDS             <1..515
                     /locus_tag="PK683_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNH08251.1"
                     /translation="DGAWNYCVRMLFDKVCRDNEENNPAMMEIHELIEKFKDFEEVDS
                     ENIGLIGQCLSGGFVLQAAMRPDLKAPVVFHHSFGFSGSGMPKTCVPKVQHKIQGHFS
                     NFDFITPKERVAELKTDLGEKLEDHYYDLPHGIPHFFELTSEGKRAYSNMLQFFRTNL
                     VSAQKDSDES"
     gene            505..1710
                     /locus_tag="PK683_07095"
     CDS             505..1710
                     /locus_tag="PK683_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH08252.1"
                     /translation="MNLKFPVLTAALIFFTMLPVTMIVPVFKEIIKDRLGGNNLAVSY
                     FMSIAMLGSFLFSPVAGYVSDRIRSRKWLISVFAVLDGISLISLTFTRDLYVLQALRF
                     AEGAFHIFVIGLLLALMADRENDPNNGRFYRKGIILGTAGTLLTLGVGLGSPMGILGK
                     KNPDLPFIIAGIIMIFIGIVSSLFLKDYEFQFHKKVTLDTWKAAYRNNPLVVIPCIYN
                     FIDRFTVGFFVSIFSLHLREDLHLNPGQTGLLLSFVLIPMSLCSYPFAVISRKTNPAL
                     WMMTGSVIYGAALGSAGFAENNYVLAFLLLVCGIGAGVMFVPSMMLVSRLVPQELNAT
                     AMTVYTGIGSIGFMLGPISAVLLDETVFSSLQKDIRFGSLALFFGMLEILTVITTLPF
                     YHRLQEKKI"
     gene            1865..>2597
                     /locus_tag="PK683_07100"
     CDS             1865..>2597
                     /locus_tag="PK683_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08253.1"
                     /translation="MQSGLKFFCILIFCGTAVQAQYHPFDTMPASAESAGLAGNSLSI
                     KDRNMNIYSNPALLAGTDRTTVSGGLYFSGKGRTTSAVLPSQAGMYVPYSENLGFGFR
                     MKSTFGNNFPYEKKVTLFSSQIFAAYRMNSFYFFAGLGPSLAYRGREQSNYSGSFSAG
                     VSYEWNRVIVSFSAERPGLNFRFRAFRESDRLQERFPDILNFALGYTVSDSIQISWET
                     KRVLYENTVYKLNDSDSRPDYERGIG"
BASE COUNT          696 a          559 c          592 g          750 t
ORIGIN      
        1 aggatggggc ctggaactac tgcgtcagaa tgctgtttga caaggtatgc agagacaatg
       61 aagaaaacaa tccagcgatg atggaaatcc acgaactgat cgaaaaattc aaggattttg
      121 aagaagtgga ttccgaaaat atcggactga tcggtcagtg tctctcaggc ggatttgtac
      181 tgcaggcggc aatgagacct gacctgaagg ctcctgttgt atttcatcat tctttcggct
      241 tcagcggaag cggaatgccc aagacctgtg tccccaaggt acagcataaa attcagggac
      301 atttcagtaa tttcgacttc atcactccga aagaaagagt ggctgagctt aagactgatc
      361 tcggagaaaa acttgaagac cattattatg atcttcctca cgggattccc catttctttg
      421 aactgacttc agaagggaaa agagcctatt ccaatatgct ccagtttttc agaacaaatc
      481 tcgtctcagc ccaaaaggat tcggatgaat cttaaatttc ctgttctgac tgccgcactg
      541 atttttttta caatgctgcc ggtcacaatg attgttcctg tattcaagga aatcatcaag
      601 gacagactgg gcggaaacaa ccttgcagtc tcctatttca tgagcattgc aatgcttgga
      661 tcattcctct tttctcctgt ggcaggttat gtttcggaca gaatccgatc gaggaaatgg
      721 ctgatttccg tctttgcagt cctcgacgga atcagtctta tctccctgac attcacaaga
      781 gatctttatg ttctgcaggc tctgcgtttt gcagaaggag catttcatat ttttgtaatc
      841 gggcttctgc ttgcgctgat ggcggacagg gaaaatgatc cgaacaacgg ccggttctac
      901 aggaagggaa tcattctggg cactgcaggc acacttctca ctctgggcgt agggttagga
      961 tcaccgatgg gaatactggg aaagaaaaac cctgacctgc cgttcatcat cgcaggaatc
     1021 atcatgattt ttattggaat tgtatcctct ctgtttctga aagattatga attccagttt
     1081 cataaaaaag tcaccttgga tacctggaag gccgcctaca gaaataatcc gcttgttgtg
     1141 ataccgtgta tatacaattt catcgacagg ttcactgtag gattctttgt cagcatattc
     1201 agtcttcatc tcagggagga tctgcatctg aatccggggc agacaggcct gctcctttct
     1261 tttgtcttga ttccaatgag tctctgttcc tatccttttg ctgtcatatc cagaaagaca
     1321 aatcctgcac tctggatgat gacaggttca gtcatttacg gagcagcgct gggaagtgca
     1381 ggttttgccg agaacaatta tgtgcttgca tttcttctgc ttgtatgcgg aatcggtgca
     1441 ggagtcatgt ttgttccttc catgatgctt gtttcaagac ttgttcccca ggaactgaat
     1501 gccacagcga tgactgtgta tacaggaatc ggttccatcg ggtttatgct gggaccgatc
     1561 tctgcagtcc ttctggatga aactgtattt tcatcactgc agaaggacat tcgattcggt
     1621 tcactggcac tctttttcgg aatgctggaa attctcacag taatcacgac tcttcctttt
     1681 tatcatagac tgcaggagaa aaaaatatga cagcggaagt ccaaagttat tttgtctttg
     1741 catttgttct gttctcggcc tataaactga ctgagccagt ctggcggtat atttacagga
     1801 aaatttttcc tgcatcaaaa aataatgaaa aatcacattc ctgctattct gaagcctgtt
     1861 ctaaatgcag agtggactga agtttttctg tatcctgatt ttctgcggaa cagcagtgca
     1921 ggcacagtac catccgttcg atacaatgcc tgcatccgca gagtcagccg gtcttgcagg
     1981 aaacagtctt tcgatcaagg acagaaatat gaacatctat tccaatcctg cgcttctggc
     2041 aggaacagac agaacgactg tctccggggg actttatttc agcggaaagg gaagaacgac
     2101 gagtgcagtc cttccctctc aggcaggaat gtatgttcct tattccgaaa atcttgggtt
     2161 cgggttcaga atgaaatcaa cattcggaaa taactttccg tatgaaaaaa aagtgactct
     2221 tttcagttct cagattttcg cagcataccg aatgaattca ttttattttt ttgcgggact
     2281 ggggccctct cttgcttaca gaggaagaga acagtccaac tactccggaa gtttttctgc
     2341 gggagtcagt tacgaatgga acagggtaat tgtttctttt tctgctgaaa ggccgggact
     2401 caatttccgg ttcagagcat tcagagaaag tgacagactt caggaaagat ttcctgacat
     2461 actgaacttt gcattaggat atacagtatc cgattctatc caaatttcat gggaaacgaa
     2521 aagagtgctc tatgaaaaca cagtttacaa gctgaatgat tctgacagca ggccggacta
     2581 tgaaagaggg atcggcg
//
LOCUS       DAOQGJ010000374         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25085_length_2596_cov_3.287682, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000374 DAOQGJ010000000
VERSION     DAOQGJ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25085_length_2596_cov_3.287682"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PK683_07105"
     CDS             complement(<1..1277)
                     /locus_tag="PK683_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNH08254.1"
                     /translation="MIQKIISIFIITACVVSADDKTDTVKKMLSDECSTKGNCGYKPT
                     HPDALKGIPIVERKLTRGMNSLPSSADLSKDMPPVQQQDGFKCVAWSTTYYLRTYLEK
                     KARNWNYYHFEGTASTDKLFSPIFVYNQINNGEDGGAAISDALNVLVKKGAALWRYMP
                     AYSGITSDVKPSPEIFKHASKYKAKEFKRIKASDLNSIKAELASGNPLVFGMAVDSGF
                     RSGLMFKPDAEPYDKSSGDPLGGHAMTLVGYDDDKKGPNGAVGAFKIINSWGPDWANE
                     GYGWITYRMFIRAQPEVYALYLNKIEKLEEDYSQEEQDGLRLKIVNPKSAQASSGTYS
                     DRIIVSWTAVPGMTKYVLFKKDLSDQNSTWERAGDAESDHFTDTNIEPDRVYKYKIFT
                     SLAKDGKNLMDNEETAPEVQGYAKSGSSPSAAVP"
     gene            1350..2237
                     /locus_tag="PK683_07110"
     CDS             1350..2237
                     /locus_tag="PK683_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08255.1"
                     /translation="MNNSFDRRKIEITLFQYNIEWDFMEKYSEKFAMILDFIIFIGSA
                     AVIGGWVIGMPLFYSPNGPVMSIFTAISLFVLVGLRLATRYFPLWPYPGTIALLMIVG
                     GGNLSSLLMLASAPSVYVNTNSSFVMTSVFTSIGFVFFSIYEIQIYLRNRTTDKVFVM
                     DDILIHLALVPGELSLIGHLVSNPTYLSMGIDPRVGISILEMCFMAALAASTVFSNKN
                     LVLWQFLKGGIGNQIIFMALFANQYIAPLLYIFFVGKKPETQAYGTELFIMLGGVFAT
                     LGFIFIQGYGPKKENLEPK"
BASE COUNT          699 a          569 c          501 g          827 t
ORIGIN      
        1 ggaactgctg cggaaggaga acttcctgat tttgcataac cctggacttc gggtgcagtc
       61 tcttcattgt ccatcagatt ttttccgtct ttggccaagg aggtgaatat tttatattta
      121 tatactctgt ctggctctat gtttgtatcg gtaaaatggt ctgattctgc atcccctgct
      181 ctttcccaag tgctgttctg atcagaaaga tcttttttaa ataagacata cttggtcatt
      241 cccggtacag cagtccagct cacaatgatt ctgtcactgt aagtacctga tgacgcctgt
      301 gcagattttg gatttacaat ctttaaccgc aacccatcct gctcctcctg agaatagtct
      361 tcttccagct tctcgatctt attaaggtaa agagcataaa cttcgggctg agctctgata
      421 aacattctgt atgtaatcca gccataaccc tcatttgccc aatcaggacc ccaggagttg
      481 atgattttaa aggcacctac tgcaccattt ggtccttttt tatcatcatc ataaccgaca
      541 agcgtcattg catggcctcc caagggatca ccggaagatt tgtcatacgg ttctgcatca
      601 ggcttgaaca tcagacctga ccgaaaacct gaatccactg ccattccgaa taccagcgga
      661 tttcctgatg cgagctcagc ctttattgag ttgagatcac ttgccttgat tcttttgaat
      721 tcttttgctt tgtatttgga tgcatgtttg aaaatctcag gagagggttt tacatcactg
      781 gtgattccgc tgtatgccgg catatatctc cacaaggctg cccctttttt cacgagcaca
      841 ttcagtgcat cagaaatcgc agctcctcca tcctctccat tatttatctg attataaacg
      901 aatataggag aaaagagttt atctgtagaa gcagttccct caaaatgata atagttccag
      961 tttctcgctt tcttttccag ataggttctg agataatagg ttgttgacca cgcgacgcat
     1021 ttgaatccgt cctgctgctg tacagggggc atatccttgc ttaaatctgc agaagaagga
     1081 agcgagttca ttcctcttgt cagttttcgt tccactatcg gaataccctt taatgcatca
     1141 ggatgagtcg gcttgtatcc gcagttgcct ttagtcgagc attcatcaga aagcattttt
     1201 ttgacagtat ctgttttgtc gtccgcagaa acgacgcagg cagttatgat aaatattgat
     1261 ataattttct gtatcaaaaa tttcccccga atatgaatgt tattttccaa caataaataa
     1321 tttcttcaat ctaaaattgc accgcttaaa tgaataattc atttgacaga aggaaaatag
     1381 aaattaccct gtttcaatac aatatagaat gggattttat ggaaaaatat tcagaaaagt
     1441 ttgcaatgat tttagatttt attattttta tcggttccgc ggccgtaatc ggaggctggg
     1501 taattggaat gcccctgttt tacagtccga acggacccgt gatgtccatc tttacagcca
     1561 tatctctctt cgttctggtc gggctccgac tggcaacaag atattttcct ctttggcctt
     1621 atcctggaac catagccttg ttaatgattg taggcggcgg caatctttct tctcttttaa
     1681 tgctggcatc cgctccaagt gtctatgtga atacaaactc atcatttgtg atgacatctg
     1741 tatttacttc catcggtttc gtattcttca gcatttacga aatccagatt tatcttcgga
     1801 acagaaccac tgacaaagta tttgtaatgg atgatatatt aattcatctt gcactggtcc
     1861 ccggtgaact cagtctgatc ggtcatttgg tcagcaatcc tacctatctc agtatgggaa
     1921 ttgatccaag ggtagggatc tccattttgg aaatgtgctt tatggcagca ttggctgcat
     1981 cgacggtgtt ttcgaacaag aaccttgtac tatggcagtt tttaaaggga gggataggaa
     2041 accagattat cttcatggca ctttttgcaa atcagtacat tgcaccgctg ctctatatct
     2101 ttttcgtcgg aaaaaaaccg gaaacacagg cctacgggac agagctcttc attatgcttg
     2161 gaggagtctt cgcaactctc gggtttattt ttattcaagg atacggtccc aaaaaagaga
     2221 acctggaacc aaaataaaaa atcaactctc atgccttgaa aaatggattg ccgctgccga
     2281 caggcaatcc gaaacttggc atctgaaacc gtttcgttca gaggagtgaa gcggcgtaag
     2341 gaattgacgt tggaaaaaac cgatgtcaca gtgcttcctc ccattaatca taatgacaga
     2401 ttcactgcaa tcatcatctt gtggcagagt tcggcaaaat caacaaccca taaagacgaa
     2461 atctgtcttg cgcggctttt caaagactct gtctttagac ctgtttcttt tccttttata
     2521 aaatacttta cttttttaat ataactccct acttcctttt attttctgta aactaaaaga
     2581 aagaaaagaa aaatcc
//
LOCUS       DAOQGJ010000375         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25135_length_2592_cov_4.290501, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000375 DAOQGJ010000000
VERSION     DAOQGJ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25135_length_2592_cov_4.290501"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..587
                     /locus_tag="PK683_07115"
     CDS             63..587
                     /locus_tag="PK683_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNH08256.1"
                     /translation="MKHRLKNLPSKMKAPFKVNRNKISLKFAIWSFISSTLSIWSILA
                     VTEWSGHPLLIGSFGASSVLLFAAPESILTQPRNLIGGHLVSAVIAVIIVKILGSSFF
                     TIGFAVGFSIFCMYLTHTLHPPGGATALIGVMGSAKPEFILVPVLAGVIILLLNALIV
                     NNIVYHRKYPTVWF"
     gene            761..2434
                     /locus_tag="PK683_07120"
     CDS             761..2434
                     /locus_tag="PK683_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08257.1"
                     /translation="MRKNLPVTSNERKIIDGAIITSKTDTKGVITYANPEFLAVSGYS
                     LEELIGKPHSIMRHPDMPEWTFEDLWSTVKNGNSWNGIVKNRCKNGDYYWVDANVSPL
                     YEAGHIIGYISVRTKPTPEQIQRAEKNYWDSAKKADKKLSSISDIKIKDIPLINGIFL
                     IFMFAVLSFLMYNTVSLLYILPALFASFFLSQTFLTLILNSKEKAIQECINDLKQIQS
                     GHLKHIIQVSRKDEMGEIQKNIRSAQVEIRGMLSQLVGSSQIINSMINELKEVFGAIQ
                     NSFDELSKAMQILAESSSVTAGSSEDTVLKISQLNANIQEIVSENLELKSESEQMNLS
                     SQKGDSVSKDAVLKFSKVLQIISAVSGDVEELGEKSKSINMIVDTITRISDQTNLLAL
                     NASIEAARAGDSGRGFSVVASEVGKLAEASRKSAKEISAFVAGLQSKISGIIHDIRDS
                     MKVLENGSKGMENIQASMKEILTHSENTFKRMENIHIITDQTKLLSDKVNKNMENIGS
                     KTSETSSVIEEISASTEEQEAAIHVISRSVADLKELSDSLNSSLTKFKF"
BASE COUNT          838 a          512 c          530 g          712 t
ORIGIN      
        1 aatctgactg aatgaatatt ttttgtgctt gtttctttat cgggatgtat caaagagaaa
       61 taatgaaaca cagactcaag aaccttccct caaaaatgaa agccccgttt aaggtgaaca
      121 gaaataagat ttctctaaaa tttgccattt ggagtttcat cagcagcact ctttccatct
      181 ggtcaattct ggcagtcact gaatggtcag gtcatcccct gctgataggt tcattcggtg
      241 catcatctgt tcttctgttc gctgctcctg aatccattct gactcagcct agaaatctga
      301 taggcggaca cctggtttct gcagtcattg cagtcatcat cgtaaaaatt ctgggatctt
      361 cattctttac aataggattt gcagtcggtt tctccatctt ctgtatgtat ctgactcata
      421 cacttcatcc tcctggaggc gcgacagcac tgatcggcgt tatgggaagt gcaaaacctg
      481 aatttattct ggtaccggtg cttgccggag ttatcatcct gcttctgaat gctctgatag
      541 tcaataatat cgtataccat cgaaagtatc ccactgtctg gttttaatta ttgaatattc
      601 agatttcata ccctacagga aggataaaat aaaaaaatga ttcacagaac ttgcatcgca
      661 gttttttctt gcataagtta attaatttat aaattgctat ttcaaaattc tgggaaaact
      721 tgagaaaact gaaaggtttc agctgcagga gcagaaaaaa gtgagaaaaa atctgcctgt
      781 tacatcaaat gagagaaaga tcatagatgg agcaatcatt acatccaaga cggatacaaa
      841 gggtgtcatt acatatgcaa atcctgaatt tctggcagta agcgggtaca gtcttgaaga
      901 actgatagga aaacctcaca gtataatgag gcatccggat atgccggaat ggacatttga
      961 agatctttgg agcactgtca aaaatgggaa ttcctggaac gggatcgtta aaaaccgatg
     1021 caagaacggt gattattact gggtagacgc aaatgtctct cctctttatg aggcaggcca
     1081 tattatcggg tacatttcag tacggactaa gcccactcct gagcagattc aaagagcgga
     1141 aaaaaattac tgggattcag caaaaaaagc tgataaaaaa ttatcttcta tctcggacat
     1201 aaaaatcaaa gacattcccc tgataaacgg aatctttctg atctttatgt ttgcagtcct
     1261 ttcatttctt atgtataata cagtttcatt attatatata cttcctgctc ttttcgcatc
     1321 atttttcctc agtcagacat ttcttacttt aatactgaac tcaaaggaaa aagccattca
     1381 ggaatgcatc aatgatctta agcagattca gtccggacat ctcaagcata tcatccaggt
     1441 aagccgaaaa gacgagatgg gagaaattca gaaaaacatc cggtctgccc aagtcgaaat
     1501 tcgaggaatg ctttcccagc tggtaggaag ttcacagatt atcaattcta tgatcaatga
     1561 gctgaaagaa gttttcggtg ccattcagaa ctcgttcgac gaactgagca aggcaatgca
     1621 gattcttgcc gaatcatctt ccgtcacagc aggaagctcg gaggatacag ttctgaaaat
     1681 atctcagttg aacgcaaata ttcaggaaat tgtatcggaa aatctggaac tgaaatctga
     1741 gtctgagcag atgaatttga gttcccaaaa aggagattct gtatccaagg atgcggtttt
     1801 aaaattttcc aaagtactgc agattatctc agcggtatcc ggtgacgtag aagagctcgg
     1861 agaaaaatcc aagtccatta atatgatagt agatacaatt accagaattt ccgatcagac
     1921 aaatctgctg gcattgaatg cttccatcga ggcagcccga gccggcgatt ccggaagagg
     1981 gttcagtgtc gtggcttcag aggtaggtaa acttgcagaa gcatccagaa aatcggcaaa
     2041 ggagatttca gcctttgtcg caggacttca gtcaaaaatt tcaggaatca tccatgatat
     2101 cagggacagt atgaaggttc tggaaaacgg ctccaaggga atggaaaaca ttcaggcttc
     2161 aatgaaagaa atactgacac attcggaaaa tacctttaaa agaatggaaa atattcatat
     2221 tatcacagat cagacaaaac tgctttctga caaagtcaat aagaatatgg agaatattgg
     2281 atcgaaaaca tctgagacaa gttctgtgat tgaggaaatc agcgcatcta cagaggaaca
     2341 ggaagctgca atacatgtca tcagccgaag tgttgctgat ttgaaagagc tttctgattc
     2401 actcaattct tctttgacga agttcaaatt ctaactcacc ttacgcaaat acaatatgct
     2461 gtctagatgc aatatttttc agaaagcatt aggaaaacct aatgaatcag cgaaaacagg
     2521 atttttaagg gcagagccct taaaaccgcc ggtaggctca cctccaaaaa cgccccacct
     2581 gcgtaaggtg ag
//
LOCUS       DAOQGJ010000376         2591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25150_length_2591_cov_4.103707, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000376 DAOQGJ010000000
VERSION     DAOQGJ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25150_length_2591_cov_4.103707"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..256)
                     /locus_tag="PK683_07125"
                     /pseudo
     CDS             complement(1..256)
                     /locus_tag="PK683_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            complement(384..653)
                     /locus_tag="PK683_07130"
     CDS             complement(384..653)
                     /locus_tag="PK683_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08258.1"
                     /translation="MDRLWVQGSFPRSFLAETEEASMLWREYGVEIKYSNAPKTSKSM
                     HTVIEDLNLEKLIVNYSGKRTYHLTEKIIVRPFSEVKKGLWFLEQ"
     gene            complement(895..>2591)
                     /locus_tag="PK683_07135"
     CDS             complement(895..>2591)
                     /locus_tag="PK683_07135"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004428736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNH08259.1"
                     /translation="ELKFPKLIGIKLTGKLSGWASAKDVILKVAGILTVKGGTGAIVE
                     YFGEGADSLSCTGKGTICNMGAEIGATTSIFSYDEKMSAYLKSSGRTDVAGLADGIKD
                     YLRGDKEVYDSPKDYFDQVIEINLSELEPHINGPFTPDLAWPLSKFAAAVKENGYPQK
                     LEVGLIGSCTNSSYEDITRAASVVQQAVEKKLKAKAEFTITPGSEQVRYTVHRDGLLD
                     TFAKIGGVVLSNACGPCIGQWTRKIDDPKRKNSIITSFNRNFAKRNDGYEGTHAFVAS
                     PEIVTLFAITGDLCFNPLTDTIVNEEGKAVKLDPPSGLELPPKGFAVEDAGYVAPVAD
                     GSGVQVAVDPKSDRLQLLDNFAAWEGTDLQGLKLLIKAKGKCTTDHISMAGPWLKYRG
                     HLDNISNNLLIGAVNFFNEKTDNVKNQLTGEYDGVPKVQRAYKAKGIGSIVVGDENYG
                     EGSSREHAAMEPRHLGVRAILVRSFARIHETNLKKQGMLALTFADKEDYNKVQEDDSI
                     DILGLTSFKEGVPLTIVLNHKDGSKDEIKANHTYNSNQIEWFKAGGALNLIKMQQK"
BASE COUNT          621 a          630 c          553 g          787 t
ORIGIN      
        1 cctcgccgcg gcgcgggctc tcgtggttga acaggatgcc gttgcacacg aacatgccgt
       61 acgcctcgcg gtagttcacg ccgatccagt gggcgtagac tttggccgcg gcgtacggag
      121 agcgcgggta gaagggcgtc gtctctttct gcggcgtctc ttgcaccaag ccgtacatct
      181 ctgacgaccc cgcctggtag aagcgcgggt tgagccccga ctcttgaatg ccttcgagga
      241 gtcgtgaatc cgcctgttct ggaaaaattc taaaaaactc atcactcaaa tgttcaacag
      301 cttctctcat ttccaatttt gcagattcca gaattttttt catgatattc ttcactttac
      361 tttatatatt atcattatac tcttcactgc tccagaaacc ataatccctt ttttacttca
      421 gagaaaggac gcacaattat tttttcagtc aggtgataag tcctttttcc agaataattg
      481 acaatgagtt tttccagatt caggtcttcg atcactgtat gcatcgactt gcttgttttc
      541 ggtgcattcg aatatttgat ttctactccg tattcccgcc aaagcatcga agcttcttca
      601 gtttcagcaa gaaatgatct gggaaaactt ccctgaaccc agagtctgtc caagtcttca
      661 ctgggaatct cattcgaagt aaaaccgcca agcggataat tcttttttgg atgagtatct
      721 atcaggaacc gcagaagagg aggtttttac agtcagaatc tctaaatata cccattatca
      781 ttgataatct tggtgatgac ccttggaaat ttcaaggata atttcgtgta gaattaaaaa
      841 aggaggctgt tccagagaat ctttcgagtc cctgaaacag ccgagaaaat ggtcttattt
      901 ctgctgcatc ttgatcagat tcagtgcacc gccggccttg aaccattcaa tctggttgga
      961 attgtaagtg tggtttgcct tgatctcgtc cttgcttccg tctttatgat tcaggacgat
     1021 tgtgagagga acaccttctt tgaaggaagt cagtccaagg atatcgatgg aatcatcttc
     1081 ctgcaccttg ttatagtctt ccttgtccgc aaatgtcaga gcaagcattc cctgcttttt
     1141 gagatttgtt tcatggattc tcgcaaatga acggacaaga atcgcacgga ctcccagatg
     1201 tctcggctcc attgcagcat gttctctgga tgaaccctca ccgtagtttt catcccctac
     1261 tacaattgat ccaattcctt ttgccttgta ggctctctgc accttaggaa ctccgtcgta
     1321 ttcaccggtc agctgatttt ttacattgtc tgtcttctcg ttgaaaaagt tgacagcacc
     1381 tatcagaaga ttgttggaga tattgtccag atgtcctctg tacttgagcc aaggacctgc
     1441 catggaaata tgatctgttg tacattttcc ttttgccttg atgagaagtt tcagaccttg
     1501 caaatcagtt ccttcccagg cggcaaaatt gtcgaggagc tgaagtctgt ctgatttagg
     1561 atctacagct acctgcaccc cgcttccgtc agcaacagga gccacatacc ctgcatcctc
     1621 tactgcaaat cctttcggag gaagttccag tcctgaagga ggatccagtt tcacagcctt
     1681 tccttcttcg ttgacgattg tatcagtcag agggttgaag cagagatctc cagtgattgc
     1741 aaacagagtc acgatttcag gagaagctac aaatgcatga gttccttcat aaccgtcgtt
     1801 tctctttgca aagtttctgt tgaatgaagt gatgatggaa ttttttcttt tcggatcatc
     1861 tatctttctg gtccactggc cgatacaagg gccgcatgca ttggaaagaa caactcctcc
     1921 gatttttgca aatgtatcca gaagcccgtc gcggtgtaca gtgtatctca cctgctctga
     1981 accgggagtg attgtgaatt ctgcctttgc cttgagtttt ttctcgactg cctgctgaac
     2041 cacgcttgct gctctagtaa tgtcttcata ggaagagttt gtgcaggaac cgatcagtcc
     2101 gacttccagt ttctgagggt atccgttttc tttgacagct gctgcaaact tggaaagagg
     2161 ccatgccagg tcaggagtaa acggtccgtt gatatgaggc tcgagttcgg aaagattgat
     2221 ttcaatcacc tgatcaaaat aatctttcgg actgtcatat acttccttgt cgcctctcag
     2281 ataatcctta atgccgtcag ccagtcctgc gacgtcagtc cttcccgaag acttgagata
     2341 tgcagacatt ttttcatcat agctgaaaat agaagtagtt gcaccgatct ctgcacccat
     2401 attgcagata gttccctttc ctgtgcagga aagtgaatct gctccttctc caaagtattc
     2461 tacgattgcg ccagtcccgc cttttacagt cagaatcccg gccactttca gaatcacgtc
     2521 ttttgcagac gcccatccgg aaagttttcc agtcagtttg attccgatga gtttcggaaa
     2581 tttcagctcc c
//
LOCUS       DAOQGJ010000377         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25213_length_2587_cov_3.366509, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000377 DAOQGJ010000000
VERSION     DAOQGJ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25213_length_2587_cov_3.366509"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..888
                     /locus_tag="PK683_07140"
     CDS             448..888
                     /locus_tag="PK683_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08260.1"
                     /translation="MNHFLNRITESRMSWKTPALICAVLLPHSLFATDMGGVIIVVVW
                     LMLDIPTFLLYLIQMIFSLYFLRKENISTFVSIINKVLFGAGIVFGAVLLFVHLVFLL
                     DMIKNAGTRNAMAFSLLIYAVILALSIFSGKKSWELMKKKSAEG"
     gene            complement(930..2066)
                     /locus_tag="PK683_07145"
     CDS             complement(930..2066)
                     /locus_tag="PK683_07145"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08261.1"
                     /translation="MNIERNLQSKIDPWVFQGKVLILFGPRRVGKTTFSKQLLEKYGK
                     ASGYFSCDTMQVQQALTKKDPFVMKDFFGEHSFIVLDEAQYVPEIGLSIKIFHDQFPN
                     IQILATGSSSFELANRTGEPLTGRSIEFFLYPFSITELQNTYTEMEAPARLENFLRFG
                     MYPEAVTHEKDKAVVYLENLISQYLYKDILIFQQLKKSEIIMRLLQLLAFQIGSEVSF
                     NELASQLKINSRTVERYIDLLEKTFILFRLKPFSRNLRKEISKKEKIYFYDLGIRNAV
                     INNFNEMELRNDTGQIWENFCIVERKKYLQAKQERRLQYFWRTHDKKEIDLIEEFSGR
                     LEGFEFKWKKNRYRIPEEFLETYPGSTVETVNSENYRNFLIWNS"
BASE COUNT          730 a          527 c          505 g          825 t
ORIGIN      
        1 cgggtatttt tccagtttct tcagaaggtc gggaacctgg gtgtcgccgc tcccataacc
       61 atgctccagc tttttccatg atttttcatc gtctaaatct atcaaaatga agtccttata
      121 attaaattat attgcatttg cggaacttct gtcccgctgc aaatatcctt atagaatttc
      181 ctgtctctat gtcaactgaa attagccaag ttatacctag atttaacagt gatgtgcccc
      241 aaaacctgat cctgtctgaa agggccatat tcagtgaaaa tgcagatctg cttttcgccg
      301 atggataatt tactgatcga ggttttgata agacagaaga ctctctgaaa aatgaatgaa
      361 tttcagaggc catatatcac tgctgttttt gaccgggact gacagatttt atcgagacat
      421 aaagcaaaac agttttattt tgggaatatg aatcattttt taaacagaat aactgaatcc
      481 aggatgtcct ggaaaactcc cgcacttatc tgtgcagtcc tgcttcctca ttctcttttt
      541 gccacggata tgggaggggt cataattgtc gttgtctggc tgatgctgga cattccgact
      601 tttttactgt atctaattca aatgatattt tcattatatt tcctgcgaaa ggaaaacatc
      661 agcacgtttg tcagcattat aaataaggtg ctgttcggtg cgggcattgt tttcggagca
      721 gttctcctgt tcgtccatct ggtcttcctt ctcgatatga tcaaaaatgc aggcacaagg
      781 aatgcgatgg cattttctct tttaatttat gcagtgatcc ttgcactgag tattttttca
      841 gggaagaaaa gctgggaact gatgaaaaag aaatcagcag agggataaga tagagttctg
      901 agccgaaggc ggtcgggcgg attttgcagt caggaattcc atataaggaa gttcctgtaa
      961 ttttcagaat tgactgtttc caccgtgctg cctgggtaag tttccagaaa ctcttcagga
     1021 atcctgtatc tgtttttttt ccatttgaac tcaaaccctt ccagtcttcc ggaaaattct
     1081 tcgatcagat caatttcttt tttatcgtgc gttctccaaa aatactgcag cctcctttcc
     1141 tgttttgcct gcagatattt ttttctctcc actatgcaga agttttccca gatctgtcct
     1201 gtatcatttc tcaactccat ctcattaaaa ttatttatta cagcattgcg gattcccaag
     1261 tcataaaaat atattttttc ctttttgctg atttcttttc ttaagttcct tgagaatggc
     1321 ttcagccgaa aaaggatgaa tgttttttcc aaaagatcaa tgtaccgttc tactgttctg
     1381 gaattgattt tcaactgtga tgccagctca ttaaacgaca cctcgcttcc aatctgaaaa
     1441 gccaaaagct gaagaagccg cattatgatc tcagattttt ttaactgttg gaatatcaga
     1501 atatctttat aaagatactg cgatatcaga ttttccagat aaactacagc tttatccttt
     1561 tcatgcgtga cggcttcggg gtacattcca aaacgcagaa agttttccag acgtgccggg
     1621 gcttccattt cagtataagt gttttgcagt tctgttatgg aaaaaggata tagaaaaaat
     1681 tcgatgctcc tgccagtcag aggctcacct gtacggttgg cgagttcaaa gctgctggaa
     1741 cctgtggcca aaatctgtat gttcggaaac tgatcgtgaa agatttttat ggaaaggcca
     1801 atttcaggaa catactgtgc ctcatctaga acaatgaacg agtgctcacc gaaaaaatcc
     1861 ttcatcacga acggatcttt tttagtcaaa gcctgctgaa cctgcattgt atcgcagctg
     1921 aaatatccgg acgcctttcc gtatttctct aaaagctgct tggaaaatgt agtctttcct
     1981 acccgtctcg gcccaaaaag gatgagaacc tttccctgaa aaacccaagg atcaattttt
     2041 gactgtaaat tcctttcaat attcatactg taatacaaaa atttatataa atctttgtat
     2101 tacagtcaat caaattttat aaaaaagtgt aacttggaaa cgagaatgat gtttgcccct
     2161 aaaagcgagc tttaatgccc cttcggatgc gaaaataccg gacacgctgt gaattcactt
     2221 tcaccatatt attgatctga cgctcgactg ccgaccgatc aatgctgtac aattagcaat
     2281 agacgctcaa aggtgttatg aggaatgatg cctcgataca attttcaccg ccggtcaccg
     2341 agtgaaaaac ccaatatttt cagggttttt cttatcgagg tgggcggtga aaatcactcg
     2401 gcaaccgtct ttcctgaata aagaacctta atttgacagc attgggcagg ggatataggg
     2461 taagcttacc tgttggaaat gcatttgtgg ggacgtcatg gcacgcatat ggcgagtaaa
     2521 tacccagatt tttaattaga ttataatagg atcaatgaaa tattggttta aaatcagttc
     2581 tggtcga
//
LOCUS       DAOQGJ010000378         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25214_length_2587_cov_3.273302, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000378 DAOQGJ010000000
VERSION     DAOQGJ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25214_length_2587_cov_3.273302"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /locus_tag="PK683_07150"
     CDS             complement(<1..1995)
                     /locus_tag="PK683_07150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08262.1"
                     /translation="MRLLFAFLLISFNLSSDTVCEYSAQDGDKEFAQADFLLSKAMEA
                     QSKAFLSRISREEKNVLLEKSAKLFSIYFECKKSQNLIIHPSSYFLNGLNFFDLSKFE
                     EAAQNAEQSLLIDPKYRDGGLLKARANIMLKDYPKALSALESIISFYPNDSDMLYLLG
                     SLSLEMGNESKALLYFGSLWNAIRKREGDAKYRLQTLKTMAEILSKKGDYSRAIYYLK
                     NYLKYKDNDYESSYFLATLYSQTGNLAESKNILLDLQKKLPHNYSIDYLLAEIYFIEN
                     KFQAYRYFNYLKQNGKLKNNSYLGALFRLMKGDYQEIKPVLEKMAEKNRIRLSLYTAL
                     SYLYKKTGETEQQIKALERAAELASVYKQYALAVELTKEKRKAVSTRPDLEEKYPKSK
                     DFEYIGISFEELGYSNLARHYMQKAEESALNGREKNNYKLRNAILLRSAKMKRYEESD
                     RILKEILSDEPDSAMVNFNLGYNQYLNTRYKESLPFYEKSILSDEKNSTYYYYRAMSN
                     EKLGKIPETVSDLKKTIELDPNFSGAYNFLGYLYAEQNMELAESIRLIQKAIDIEPDN
                     PSYQDSLGWVLYRLSRYEEALHHLILAKEILEEKKEEDAAVFDHLAEVYVKLKEPENA
                     REHWKRALELYTDLKEKNRVRQRLEEIGEKLDLTRERIRQI"
     gene            2110..>2587
                     /locus_tag="PK683_07155"
     CDS             2110..>2587
                     /locus_tag="PK683_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08263.1"
                     /translation="MKKILRDNAVFIFALFIFWGLCFPAVYYYRQSLYDWFVIKVSPS
                     GYSPKVMNGFIKDGDRIISKLILDRREKDEKKFMDETDQFLKKMKLACEYYQEVSSRD
                     EVLADPTWYEKNTVWTSDNSAFTKVDGVSRNGLGEGTVPKEFWKESIGEILSALDYY"
BASE COUNT          678 a          520 c          560 g          829 t
ORIGIN      
        1 aatctgacga atacgttctc tcgttaaatc gagtttttct ccaatttctt caagtctctg
       61 tctgactctg tttttttcct tgagatccgt ataaagttcc aatgctcttt tccagtgctc
      121 tctggcattt tccggttctt tcagttttac ataaacctcg gcaagatgat cgaatacagc
      181 ggcatcttcc tctttttttt cttccaggat ttcctttgcg agaatcagat gatgcagtgc
      241 ttcttcatat ctggagagtc tgtaaagaac ccatcccaga ctgtcctggt aggacggatt
      301 gtccggttca atgtctatcg ccttctggat cagccggatc gattcggcaa gttccatatt
      361 ctgttcagcg taaagatatc ctaagaaatt gtaggccccg ctgaagttgg gatcgagttc
      421 aattgtcttt ttcagatcag acactgtctc cggtattttt ccaagtttct cattggacat
      481 tgccctgtaa taatagtatg tggaattttt ttcgtcggag agaatcgact tttcataaaa
      541 cggcaggctt tccttgtacc gggtgttcag atactgattg taacccagat taaaattgac
      601 catggccgaa tcgggttcat cactcagaat ttctttcaga atcctgtcgg attcttcata
      661 tcttttcatt tttgcagaac ggagaagaat cgcattcctc agcttgtaat tgttcttttc
      721 tcggccgttc agagcagact cttcagcctt ctgcatatag tgtctggcaa gattggaata
      781 gcccagttct tcaaatgaga ttccaatata ctcaaagtct tttgacttag ggtatttttc
      841 ttccagatcg ggtcttgtac tgacagcctt tcttttttcc ttagtgagtt cgactgcaag
      901 ggcatactgc ttataaactg atgcaagttc agctgctctt tccaaggcct tgatctgctg
      961 ttccgtctct ccagtctttt tgtagagata cgagagagct gtgtagagtg aaagtctgat
     1021 ccggtttttc tctgccattt tttccaggac aggcttgatt tcctggtaat cacccttcat
     1081 cagacggaaa agtgcaccga ggtaactgtt gttcttcagt ttgccgttct gcttcaggta
     1141 attaaaatac ctgtatgcct gaaatttatt ttcaataaaa tagatctctg ccagaaggta
     1201 gtcaatagag tagttgtgcg gcagtttttt ctgaagatcc aggagtatgt tcttggattc
     1261 tgccagattt cctgtctgac tgtacagagt agccagaaaa taactggact cgtagtcatt
     1321 gtccttgtac ttcagatagt ttttcagata gtagattgcc ctggaatagt ctcctttttt
     1381 actgagaatt tctgccattg tcttcagtgt ctggagtctg tatttcgcat ccccttcacg
     1441 cttgcgtatt gcattccaga gggagccgaa ataaagaaga gctttggact cgtttcccat
     1501 ttcgagagag agactgccta aaaggtacag catatccgag tcattcgggt aaaaggaaat
     1561 gatgctttcg agtgcggaaa gtgccttggg atagtcttta agcattatat ttgctctggc
     1621 cttcagaagt cccccgtccc tgtactttgg atcaatcaga agagactgct ctgcattctg
     1681 agctgcctcc tcaaatttgg agagatcaaa aaaattgagt ccattcagaa aataactgct
     1741 gggatgaatg atcagattct gcgacttttt gcattcaaag taaatggaga aaagttttgc
     1801 cgatttttcg agcagaacgt ttttttcttc cctggaaatt ctgcttaaga acgccttgga
     1861 ctgcgcttcc atcgctttgg aaagaaggaa gtccgcctga gcaaattcct tgtcaccgtc
     1921 ctgagcggag tattcgcaga cagtgtctga acttaggttg aaggagatta aaagaaaggc
     1981 aaaaagtaga cgcatatagt attttcagac tgttctgttt ccttcagaag taaatcaaat
     2041 tttccggaaa atttaaaaag tttgcagaat ttatgcatga gagacgaata gtactgaagg
     2101 aagatttctt tgaaaaaaat tctcagagac aatgctgtat ttatttttgc cctttttatt
     2161 ttctggggac tctgtttccc tgcagtttat tattacagac agtctctgta cgactggttt
     2221 gtcattaagg tttctccttc cggatattcg cccaaggtga tgaacggctt cattaaggac
     2281 ggagacagga ttatttctaa actgattctg gacagaaggg aaaaagatga aaaaaaattt
     2341 atggatgaaa cagatcagtt tctgaaaaaa atgaaacttg cctgcgagta ttaccaggaa
     2401 gtcagcagca gagatgaggt acttgccgat ccaacctggt atgaaaagaa tacagtctgg
     2461 acatcggaca attctgcatt caccaaggtc gacggggtaa gcaggaacgg attaggtgaa
     2521 ggaactgttc ccaaggaatt ctggaaggaa agcataggag aaatcctgag tgcactggac
     2581 tattata
//
LOCUS       DAOQGJ010000379         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25240_length_2586_cov_2.753852, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000379 DAOQGJ010000000
VERSION     DAOQGJ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25240_length_2586_cov_2.753852"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..519
                     /locus_tag="PK683_07160"
     CDS             64..519
                     /locus_tag="PK683_07160"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNH08264.1"
                     /translation="MADKRILNFSSYSDIKKEIESLHKGGYKQGGKWNLGQICRHLSY
                     YYKGALDGFSSKMPWIIRVTIGRMVKKSLLKQTSYRQGMQTDPKSVYNQEPDEKAAVQ
                     EILGLLDRLEKNASPLHPSGFFGEMTNDEWKTMNLGHAAHHLGFLYPNS"
     gene            complement(577..1905)
                     /locus_tag="PK683_07165"
     CDS             complement(577..1905)
                     /locus_tag="PK683_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNH08265.1"
                     /translation="MELNSLDLWFRRILLWLTIASGIVQYAWINNVWTNGIFIVLMAG
                     IVGYYTNFLAIKMLFQPKQGKVLGWEGLVPKNKPKIAKSLGESIQNRLLAPEIILNYI
                     YEKNLIEIGTRKISTWIDETLANEKVRHTISTKIISVIREKGPEALSSAFDISEDVLK
                     KIAQNPDEIIKYWGIARERIIDYIKSEENREKIGFMLKKVMLEEMPKLSTALNAAIDG
                     YLANKKTFGTSIGKSLKKLVSFDDEAIKDLLEKFVKDPETGEQFMGILDTLVVEIQTK
                     LNAPETQNYIISQVGSWVNDLSSYARQNVLPAGIERLQEILNDEKYWKKLDEYSFRAM
                     EWVKDKSLEFANSAEGQEYLKTYIGKFVHQINVTQLVEEQVMKLDTDDLEKMILDNTG
                     GNLVVIQILGGVLGLIAGFIQVNVLFSIPVGALVAVIVFSYNRNRIKYQD"
     gene            complement(1972..>2586)
                     /locus_tag="PK683_07170"
     CDS             complement(1972..>2586)
                     /locus_tag="PK683_07170"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08266.1"
                     /translation="TGTLLLNFPEMPDMRISSQTFEKISFSRKNIHYKNCIETAKLIL
                     LNDHSDRKKGKNSGFALMTELNDLWKKFIYKSILEYRKRDFSITVRTAGSLWKSEYSD
                     RKKIKPDIILNKGSENCIVLDTKWKNSPSSEDFRRLHSASEIFGSDKTAFLLPGEKSL
                     NGFFLNSRQGRQSEKICRIIRIPADINVEQWRKNISSSVDEWMD"
BASE COUNT          674 a          577 c          517 g          818 t
ORIGIN      
        1 tgtccctaaa tgaaaaatca ttgcatcgga aaaaggaaga ttttttctga atcagaggat
       61 attatggcag acaagagaat tttaaacttc agttcctatt ctgatatcaa aaaagagatt
      121 gagtctcttc acaagggcgg ttataaacag ggcggcaaat ggaatctggg acagatttgc
      181 agacatctgt cgtattatta taaaggcgca ctggacggtt tttcatccaa gatgccctgg
      241 ataatccgtg ttacaatcgg caggatggtg aagaaaagcc ttctgaagca gacttcatac
      301 agacaaggta tgcagactga tcctaaatca gtctataatc aggaacctga cgaaaaagcg
      361 gccgttcagg aaatacttgg acttctggac agactggaaa agaatgcgtc tcctcttcat
      421 ccgtcgggtt ttttcggaga gatgacaaac gatgagtgga aaaccatgaa tcttggacat
      481 gctgctcatc atctgggttt tctgtatccc aattcgtaga cacgggtttt gtgccgtaga
      541 gacgggtctc agacccgact ctacgactct gtgcgttcaa tcctggtatt tgattctgtt
      601 cctgttgtaa gagaacacta tgactgcaac aagtgctccg acagggatcg agaacagcac
      661 attcacctga atgaagccgg cgatgagtcc aagcactcct cctaaaatct gaatgactac
      721 gagattccct cccgtattgt ccagaatcat tttttccaga tcgtctgtat cgagcttcat
      781 cacctgttct tccactagct gagtcacatt gatctgatgc acaaattttc ctatgtacgt
      841 tttcagatat tcctgaccct ctgccgagtt tgcaaattcc agagacttgt cctttaccca
      901 ttccattgca cgaaatgaat attcatccag cttcttccag tatttttcat cattcagtat
      961 ttcctgaagc ctttcaattc ctgcaggaag aacattctgt cttgcataac tcgaaaggtc
     1021 gttcacccag ctccctactt gggatataat ataattctgt gtttcaggcg cattcagctt
     1081 ggtctgaatc tcaaccacaa gcgtatcgag aattcccata aactgttctc ctgtttcggg
     1141 atctttcacg aatttttcca gcagatcctt gattgcctca tcatcaaagg ataccagctt
     1201 tttcaggctc ttgccgatag atgtgccgaa agtcttcttg tttgcgagat acccgtcaat
     1261 tgccgcattc agcgcagtgg aaagtttagg catttcttcc agcatgactt ttttcagcat
     1321 aaagcctatc ttctctctgt tttcctccga tttgatatag tcaatgatcc tctctctggc
     1381 aatcccccag tacttgatga tttcatccgg attctgtgcg atttttttca gcacatcctc
     1441 tgaaatgtca aacgcactgg acagtgcctc gggacctttt tccctgatga cagatattat
     1501 cttagtactt atagtatgac gtactttttc atttgccagt gtttcatcaa tccaggtgga
     1561 gatttttctg gtcccgattt caatgaggtt cttttcatat atgtaattga ggatgatttc
     1621 cggagcaagg agcctgttct gaatgctctc tcccagactt ttggcaatct tgggcttgtt
     1681 cttcggcaca agaccttccc agccgagaac ctttccctgc ttgggctgaa aaagcatttt
     1741 gattgcaaga aaatttgtat aatagcctac aatccctgcc atcaggacaa tgaaaattcc
     1801 gtttgtccag acattgttga tccaggcata ctgcacaatt cctgaagcga ttgtgagcca
     1861 gagaagaatt ctgcgaaacc agaggtcgag tgaatttaat tccatattga cgtcggttag
     1921 tatatttcct catggctggc cgtcaatgaa aaaagcagct tatttgacgg gtcagtccat
     1981 ccattcatct acagaagaag atatgttttt ccgccattgt tcaacattta tgtccgcagg
     2041 aattcggatg attctgcaga ttttttccga ttgtctgcct tgccgggaat tgaggaaaaa
     2101 cccattcaga gacttttccc cgggaagaag gaatgcagtt ttgtccgagc cgaaaatttc
     2161 cgatgcagaa tgcagtctcc tgaagtcttc actggaagga gagtttttcc attttgtatc
     2221 cagtacgatg cagttttcac tgcccttatt caatatgata tccggtttta tttttttcct
     2281 gtctgaatat tccgatttcc acaaagatcc tgctgtccgg actgttatgg aaaaatccct
     2341 cttccggtat tcaagtatgc ttttatatat aaattttttc cataagtcat tcagttcagt
     2401 catcagtgca aatccggaat tttttccctt tttccgatca gaatgatcat tcagaaggat
     2461 cagcttagca gtttcgatgc agtttttata atgaatgttt tttctgctga aactgatttt
     2521 ttcaaatgtc tgactgctga ttctcatatc aggcatttcc ggaaaattca gaagaagagt
     2581 gcctgt
//
LOCUS       DAOQGJ010000380         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25291_length_2583_cov_3.517009, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000380 DAOQGJ010000000
VERSION     DAOQGJ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25291_length_2583_cov_3.517009"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK683_07175"
     CDS             <1..185
                     /locus_tag="PK683_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH08267.1"
                     /translation="AQDLIAKHGYRPRNKKILQKYASKFPNIELLTIDSHFGGWKKAQ
                     TFHFADRGTFDQLYVK"
     gene            194..1150
                     /locus_tag="PK683_07180"
     CDS             194..1150
                     /locus_tag="PK683_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08268.1"
                     /translation="MKFYKSFDYSGRHLAETRGAHGASLLRGFVQTVFFIPLLIGCSN
                     FQDLGVAPKNKNNNVALQALSYAVGKNIPNVGPLSICGASAITPIANAAVTSPYSTQY
                     NLLNCSPSLISGFTSKNITTSTRGLSGTSSSSTIISDGAQVGSGGVSGKSNIEVSFFI
                     GTSSGSLDIISYGDGASFSGPAWRITAAQNQYKDQNGTSSSSQIAYKSQDGVFRVPVS
                     GSTPAVPTNPIKTVSSGTTARTVCLDFHASAGTLQAAWDGTCSSVSETKKTKAAFPIS
                     IDPADIAVSTSAPLTPVGQRIGFILNNVTITNFTVSSQLSLE"
     gene            complement(1204..1488)
                     /locus_tag="PK683_07185"
     CDS             complement(1204..1488)
                     /locus_tag="PK683_07185"
                     /inference="COORDINATES: protein motif:HMM:NF021563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2288 family protein"
                     /protein_id="HNH08269.1"
                     /translation="MDQHREEIRSGIAEIAWDELQLFYAKGQVIQIHSDMDLEEVGYQ
                     LCMDNASLLETWIREGLVGRISDDTARNWIDSKAKVRSVVIQPWVLVQEL"
     gene            complement(1502..2011)
                     /locus_tag="PK683_07190"
     CDS             complement(1502..2011)
                     /locus_tag="PK683_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08270.1"
                     /translation="MWSGAIAMQGIGINRKDIRAFVEYMLKNGTAEEKENCLSYMKSD
                     MEIDSPSGIDNPEISLSSFLLESNYLFATTLGKLGLTLHTYPHDLRDRYEENTVGVIG
                     HDICTLYSGDEPPVTAEPDEETLKENLAVIFGKKRIRTAPLKDETEFLITKGYFSYYS
                     FPADCMCCT"
     gene            complement(2159..2470)
                     /locus_tag="PK683_07195"
     CDS             complement(2159..2470)
                     /locus_tag="PK683_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08271.1"
                     /translation="MMNRDFLEKVIPNYNGNTRVLAETLDGLELTLVYETPDLQDDQF
                     KDFATGFVFRRDELKNFKNCSDYTTHIKTKNHLARNHYSYIHSGKNHKSDSHNLDKLI
                     K"
BASE COUNT          707 a          607 c          520 g          749 t
ORIGIN      
        1 ctgcacagga tctgattgca aagcacggat accgtccaag aaacaagaag attcttcaga
       61 aatatgcttc caaatttccg aacatagaac tgctcacaat tgattctcac ttcggaggct
      121 ggaagaaggc acagactttc cattttgctg atcggggaac attcgatcag ctgtatgtaa
      181 aataggagac cctatgaaat tttataaatc ttttgattat tcagggaggc atctcgcgga
      241 aacgcggggt gctcacggtg cttcactcct gcgaggcttt gttcagacag tattcttcat
      301 accactgctt atcggatgca gcaattttca ggatctggga gtagcaccga agaacaagaa
      361 caataatgtc gctcttcagg cactttccta tgcagtcgga aagaacattc ctaatgtagg
      421 cccgctttcc atctgtggtg catctgcaat cacaccgatt gcaaatgcag cagtgacatc
      481 tccatacagt actcagtaca atctgttaaa ctgcagtccc agtctgattt caggattcac
      541 gtcaaagaac atcacaacat cgacaagagg gctttcgggg acttcctcct caagcacaat
      601 catttctgac ggtgcccagg tgggttcagg aggagtatca ggaaaatcaa atatcgaagt
      661 gagttttttc ataggcacat catccggttc tctggacatt atttcctacg gggacggggc
      721 ttctttttcc ggtccggcct ggaggatcac tgcagcacag aatcagtaca aggatcagaa
      781 cgggacaagc agttcttcac agattgcata taaatcacag gatggagtat ttcgtgtgcc
      841 tgtaagcggt tccactcctg ctgtaccgac aaatccgatc aagacagttt cctcaggtac
      901 aactgcaaga actgtctgtc tggactttca cgcaagtgca ggaactcttc aggcggcctg
      961 ggatggaacc tgctcttcag tttcagaaac aaagaaaaca aaagctgcat ttccgatatc
     1021 catagatccg gctgacattg cagtttcgac ctcggcacca ctcacgcctg tcggtcaaag
     1081 aataggattt attctaaata atgtgactat aaccaacttt acagtatcct cacagctctc
     1141 tttagaatag attaattttt ccccattttc aagacttgat acggacagaa atgtccgttt
     1201 tttttaaagt tcttggacga gaacccaggg ctgaatgact acagatcgga ctttggcttt
     1261 ggaatcaatc cagttccttg ctgtgtcatc agaaattctg ccgactaagc cttcacgtat
     1321 ccaagtttcc aaaagagatg cattgtccat acagagctga taccctacct cttcgagatc
     1381 catatccgaa tggatctgta tcacctgccc ctttgcataa aaaagctgga gctcatccca
     1441 ggcaatctcc gcaatgcctg aacgaatttc ttccctatgc tgatccattc agtctaagca
     1501 atcaggtaca gcacatacag tcagctggaa aagaatagta actgaaataa ccctttgtga
     1561 tcagaaactc ggtctcatcc ttcaaaggag cagtcctgat tctctttttc ccgaagatca
     1621 cagccagatt ttctttcaat gtttcttcat ccggctctgc agtgaccggc ggttcatcac
     1681 ctgaatacag tgtacagatg tcgtgaccta tgactcctac tgtattctct tcgtatctgt
     1741 ccctaaggtc atgcggatac gtatgtaaag tgagacccag cttcccgaga gtagtcgcaa
     1801 aaagataatt ggattcgaga agaaaagaag agagactgat ttctggattg tcaataccgc
     1861 tcggcgaatc gatttccata tctgatttca tataggaaag acagttttct ttttcttctg
     1921 ctgttccgtt tttcagcata tattccacga aagcacggat atcctttctg ttgatcccta
     1981 tgccctgcat cgcaatagca ccactccaca tataataaac ctaccttata atctattgac
     2041 tcatcttacg ccgcgagtcg gggcgatctt gacagggtct gcctcaagac cgtccaatgg
     2101 cttcgccctt gaattctttt ttactgattt acaagctttc tgcaatgcac ttgcagagtt
     2161 acttgatcag tttatccaga ttgtgactgt ctgatttatg attttttcct gaatgaatat
     2221 aggaataatg atttctggca agatggtttt ttgtcttgat atgagttgta taatcagagc
     2281 agtttttgaa atttttcagc tcatctcttc tgaatacgaa cccagtggca aaatctttga
     2341 actgatcatc ctgcagatca ggtgtttcat atacaagtgt aagctccagc ccgtcaagtg
     2401 tctctgccaa aactcttgta ttgccgttat aatttgggat taccttttct aaaaaatcgc
     2461 gattcatcat aatttgttcc tgtttccaga ttcaatcctc acgaaccgaa tccggaaatc
     2521 aacagttttt agagaaaatc tttgagcagt ttgatgccgg tagcaagaag tccgccgctt
     2581 tcc
//
LOCUS       DAOQGJ010000381         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25378_length_2578_cov_3.487119, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000381 DAOQGJ010000000
VERSION     DAOQGJ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25378_length_2578_cov_3.487119"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="PK683_07200"
     CDS             <1..1893
                     /locus_tag="PK683_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08272.1"
                     /translation="PHITISASYPVYSRNGELRGVLGIDQELKYLNFYLAEMSFKKGK
                     VFVMDRAGNLIAASSGEPLTRKNGSNEVRVHSSESREKLISETSLHIQKHHFNFKDIV
                     DNTAGSYFNSAGEVFYIIKNFSNNMGIDWLIVIAIPESEFLSDIHDNTRTAVFLSIAA
                     VLFSFLSSLLIGKKIIQPILRLNTAARGIAEDKLLTVDDTDRNDELGELTRSFSIMAG
                     KLSESFRNLEEKVQSKTNELQLAKEKAEAANRAKDIFLANMSHEIRTPLNGILGMIEL
                     LQSRKLDAEGREFLRIMSLSGKSLLGIINDILDLSKIESGKIELLSIKLNLESIVREI
                     GDIFALEIKKKNIQMIIDTDSSLPQFLEGDPDRIKQVLLNLVGNAVKFTEKGTVRISV
                     RVLKIRDEKAEIEIRIEDSGIGISSEKMEKLFQPFSQGDSSTKRKFGGTGLGLVISRS
                     LVEHMNGTVSVQSEEGRGSTFCIRVILKVLPSGAEETPRISDEILLRGRRPRLLIVED
                     NEINLLVLENLAEKYASRIVTAKSGEEALLKMDKELFDIVFTDIQMPGMDGIELTGNI
                     KRKFRTNCPYIVAVTANAFTEDKKKYLAFGMDAYLSKPVSGESISRLVLNWMLVNEKR
                     KDHEEH"
     gene            1862..>2578
                     /locus_tag="PK683_07205"
     CDS             1862..>2578
                     /locus_tag="PK683_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08273.1"
                     /translation="MKKGKIMKNIETVASLKESGYAERPVKREIAFNLLTKLKKKEKI
                     FQGIHGYEETVIPQLVNAILAGHNIALLGEKGQAKSRIMRSLTDLLDEYIPVLRDTEI
                     PESPFHPITGKAKEILWREGDNAGIKWIHRSERYGERLAPGAKIADIIGDIDPSKIIG
                     GEALSSEGAIFFGILPKMNRGIFAVNELPDLDYLVQVSLFNILEENDIQIRGIPIRFE
                     LDVMVCFTANPEDYARSGKII"
BASE COUNT          807 a          485 c          627 g          659 t
ORIGIN      
        1 ccgcatatca ctatttccgc gagttatcct gtgtattcca ggaacggtga gctgagaggc
       61 gttctgggaa tagatcagga actgaaatat ctgaatttct atctggcgga aatgagcttc
      121 aagaaaggaa aggtgtttgt gatggacagg gccggcaatc tgattgctgc atcttcagga
      181 gaaccgctta ccagaaagaa cggttcaaac gaagtgagag ttcattcttc tgaaagcagg
      241 gagaagctga tttcggaaac cagtcttcat atccagaagc atcatttcaa tttcaaggac
      301 atcgtggaca atactgcagg aagctatttc aacagtgcag gagaagtttt ttatattata
      361 aagaattttt ccaataatat gggaattgac tggctgatag tcattgcaat tccggaatcc
      421 gaatttcttt cagacatcca tgacaataca aggacggcag tttttctgag cattgctgca
      481 gttctttttt catttctttc aagtctcctg atcggaaaaa aaatcatcca gccgattctc
      541 aggctgaata ctgccgcaag ggggattgct gaagacaaac ttcttactgt ggatgatacg
      601 gaccgcaacg atgaacttgg agaactgacc agatccttca gcattatggc aggcaagctt
      661 tctgaatctt tccgcaacct cgaagaaaag gtgcagagca agacgaatga gcttcagctc
      721 gcaaaggaga aggctgaggc tgcaaacagg gcaaaggata tttttcttgc aaatatgagt
      781 catgaaatca ggactcctct gaacggaatt ctcggcatga ttgaacttct gcagtcgaga
      841 aagctggatg cagagggcag ggaatttctt cgtattatga gtctcagcgg taaaagcctt
      901 cttggaatca tcaatgacat acttgatctc tccaagattg agtcgggaaa gattgaactt
      961 ctgagcatta agctgaatct ggaatccatt gtcagggaaa ttggggatat atttgcatta
     1021 gaaatcaaga aaaaaaacat acaaatgatc attgatacgg actcttctct tcctcagttt
     1081 ctggaaggcg atcctgacag aatcaagcag gttcttctga atcttgtagg gaatgcagtg
     1141 aagtttaccg agaagggcac ggtccgaatt tcggtcaggg ttctcaaaat cagggatgaa
     1201 aaggctgaga ttgaaatcag aatagaagac tcaggaatcg gaatttcttc agagaaaatg
     1261 gaaaaactct ttcagccgtt ttcgcaggga gattcatcta caaagagaaa attcggcggg
     1321 accggtctcg gtcttgtgat ttcgagaagt ctcgtggagc atatgaacgg aactgtttca
     1381 gtgcagagtg aggagggcag aggaagcact ttctgcatca gggtaatact gaaggttctc
     1441 ccttccggtg cagaagaaac tcccaggatc agcgacgaaa ttcttctcag aggaagaagg
     1501 cccagactgc ttatcgtgga agacaacgaa atcaatcttc tcgtccttga aaatcttgca
     1561 gaaaaatatg catccagaat tgttactgca aaaagcggtg aagaggctct tctgaagatg
     1621 gacaaagaac tcttcgacat tgtatttaca gacatacaga tgcctggaat ggacggtata
     1681 gaacttacag gcaatatcaa gagaaaattc agaacgaact gtccttatat tgtggcagtc
     1741 actgcaaacg catttactga agacaaaaag aaatatcttg catttggaat ggatgcttat
     1801 ctgagcaagc ctgtcagcgg cgaatccata tccagacttg tgctgaactg gatgcttgtg
     1861 aatgaaaaaa ggaaagatca tgaagaacat tgaaacagtt gcatcattga aggaatcagg
     1921 ttatgcggaa aggcctgtca aaagagaaat tgcatttaac ctgctcacaa aactgaagaa
     1981 gaaggaaaag atttttcagg gcatacacgg atatgaagaa actgtcattc ctcagcttgt
     2041 gaatgcaata ttagccggac ataatattgc tcttctggga gagaaggggc aggcaaagag
     2101 caggattatg cgttcactga ctgatcttct ggatgagtat attcctgtgc tgcgcgatac
     2161 tgaaatccct gaaagtccct tccaccctat taccggaaag gcaaaggaaa tcttatggag
     2221 ggaaggagac aatgccggaa tcaaatggat tcacagatcg gaacggtatg gagaaagact
     2281 ggctcccggt gcaaaaattg cagatatcat aggggatatt gatccttcca aaatcatagg
     2341 cggagaagct ctttcttcgg aaggggcaat ctttttcggg attctgccga agatgaacag
     2401 aggaattttt gcagtcaatg aacttcctga tctggattat cttgtgcagg tctcattatt
     2461 taatatactg gaagaaaatg atattcagat acgcggtatc ccaatccgct ttgagctgga
     2521 tgtaatggta tgcttcactg caaatccgga agactatgcc aggagcggga aaattatt
//
LOCUS       DAOQGJ010000382         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25457_length_2573_cov_2.848689, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000382 DAOQGJ010000000
VERSION     DAOQGJ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25457_length_2573_cov_2.848689"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="PK683_07210"
     CDS             complement(<1..1597)
                     /locus_tag="PK683_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNH08274.1"
                     /translation="MSDILRKETDRRRTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGA
                     VKARKDRRSVTSDWMEMEKERGISITSAALQFEYKGFVLNLLDTPGHEDFSEDTYRTL
                     IAADTAVMVLDAAKGVEPQTRKLFKVCRERGIPIITFVNKLDRPTKDVFALLDEIEDV
                     LGITAVPQVWPIGTGPDFRGVYDRKEKQVHLFEKTPGGAFQAPVQTGGINDSILESKI
                     DMDILSKCREEIDLLETGIEGFDKEKFLSGQISPVFFGSAVNNFGIQLFLDNFLELAP
                     SPYHVPLSDGKFLDPLSSSFSCFVFKVQANMNRVHRDRIAFVRICSGKFERGLSVKHE
                     RLEKPVKLSSSFAFFGQDRNTIDEAYPGDIIGLINPGTFRIGDILYSGEAPKLKPLPV
                     FPPELFATISCTDSLSLKSFKKGIEQLSEEGIIHLFTSKYIGGGNPIIGAMGVLQFEV
                     FQRRLKDEYNAVSRVTNLPYEICRWILEKDVSSLPSSANAYTMQDKEGRTAIMFEDEW
                     SLNYFVKNNPDISLLEHPLGNSDT"
     gene            1929..2237
                     /gene="yajC"
                     /locus_tag="PK683_07215"
     CDS             1929..2237
                     /gene="yajC"
                     /locus_tag="PK683_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004484627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNH08275.1"
                     /translation="MIFLAQAAEGAPEDPVKKILSTLLLPAIIFIMMWLLVIRPYRKE
                     EKTRKDMITSLAKGDTVVTSSGIHGKVVEFRDNNETVVLNISKDTNVVFNTSTITKKK
                     "
     gene            2240..>2573
                     /locus_tag="PK683_07220"
     CDS             2240..>2573
                     /locus_tag="PK683_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08276.1"
                     /translation="MFRFLLALILIFSATFAEDSDRRRKNNANANKKKTASSSKDRKN
                     SKDKKKDQENPKRLSSAEIRELNANWVHPELNCLYEPTGKEDLPIPEKTATAEIKPKP
                     EEVKPKPNI"
BASE COUNT          746 a          562 c          549 g          716 t
ORIGIN      
        1 cggtatcaga attccccaaa ggatgttcca gcagactgat gtcaggattg tttttcacaa
       61 aataattcag actccattca tcttcaaaca taatggcagt tctgccttcc ttgtcctgca
      121 ttgtatatgc atttgcagaa gatggaagag agctcacgtc tttttctaaa atccacctgc
      181 agatttcata gggaagattt gtcactctgg aaactgcatt gtattcatct ttcagtcttc
      241 tttgaaacac ttcgaactga aggactccca tagcaccgat gataggattt ccgccgccga
      301 tgtactttga agtgaagaga tgaatgattc cttcttcaga aagctgttct atcccttttt
      361 tgaaagattt taatgataaa ctgtctgtac agctgattgt ggcaaaaagc tcaggcggaa
      421 acactggaag gggtttcagc ttaggagctt ctccgctgta caggatgtct ccgattctga
      481 aagttcccgg attgatcagt cctataatat cgcccggata ggcttcatcg attgtatttc
      541 tgtcctgccc gaaaaatgcg aaggaagagg aaagcttcac cggtttttcc agtctttcat
      601 gttttacaga aagacctctt tcaaatttgc ctgaacagat tctgacaaat gcaatcctgt
      661 ccctgtgcac tctgttcata tttgcctgta ccttgaagac aaaacaggag aacgaggatg
      721 agagggggtc aagaaatttt ccgtcactaa gtgggacatg gtatggcgaa ggagcaagtt
      781 ccagaaaatt gtccagaaaa agctgtatcc cgaaattatt gactgccgat ccaaaaaata
      841 caggagagat ctgaccggaa agaaatttct ccttatcaaa accttcgatt cctgtttcca
      901 gaaggtcaat ttcttcgcgg catttggaaa ggatatccat atcaattttg gattcgagga
      961 tgctgtcatt gatcccgccg gtctgaaccg gggcctgaaa tgcaccgcca ggagtctttt
     1021 caaaaagatg cacctgtttt tcttttctgt catagactcc tcgaaaatca ggtccagttc
     1081 ctatcggcca gacctgcgga accgctgtaa ttccgagtac gtcctcgatt tcatccaaaa
     1141 gtgcaaagac atccttggtc ggtctgtcca gtttgtttac gaaagtaatg attggaattc
     1201 ctctttcgcg gcagaccttg aaaagctttc tggtctgagg ctccacccct ttggccgcgt
     1261 ccagaaccat tactgcagta tcagcagcaa tgagcgtcct gtaagtatcc tcagaaaagt
     1321 cttcgtgtcc gggagtatcc agaaggttca gaacgaagcc cttgtattca aactgaaggg
     1381 cagcagaagt gatggagatc cctctttcct tttccatttc catccagtcg gatgtgactg
     1441 acctacggtc ttttctggct ttgactgcac ctgccagctg aattgcacct ccgtaaagaa
     1501 gcagcttttc agtcagcgtt gtctttccgg catccggatg ggcaataatg gcaaatgtcc
     1561 ttctgcggtc cgtttctttt ctgagaatat cactcataga taccagaatt tttttgttat
     1621 ctacaggtgc aatagtaagt tcttttcaga tggacggaac aatttgttcc tctgcaattt
     1681 tgccgactga tttaccggca gctgatttcc cctctgcctg atctctgcag ttttagcatt
     1741 tccaatactg cagaaggtga aagagcagtc tgaacgaaca tattatgaaa acgcagggga
     1801 aagatagatc gggaagaaaa aagaggagtt tccctgtccc caaagctcta tttttcgtgg
     1861 aatattattt tacttgaaaa aagatgcctg aatttcaggt ttgttgtatg attttagcat
     1921 tccaaatgat gatttttttg gcacaggccg cagaaggtgc tccggaagat ccagttaaaa
     1981 aaattctatc tactcttctt cttcctgcga ttatttttat tatgatgtgg cttctggtaa
     2041 tcagacctta cagaaaggaa gaaaaaacca gaaaagatat gattacaagc cttgccaaag
     2101 gtgatacagt ggtcacgtcc agcggaattc acggtaaagt cgtcgaattc agagacaaca
     2161 atgaaactgt tgtgttgaat atttccaaag atactaatgt agtgttcaat acgagcacaa
     2221 tcactaagaa gaaataacaa tgttccgctt tcttctggca ctgattctga ttttttctgc
     2281 taccttcgcc gaggattccg accgcaggag aaaaaataat gcgaacgcta ataaaaagaa
     2341 aacagcttcc agttcaaagg acaggaaaaa cagcaaggac aaaaaaaagg atcaggaaaa
     2401 tcccaaaaga ctgtcttcag cagaaattcg ggaactcaat gcgaactggg ttcatcctga
     2461 gctgaattgc ctttatgaac cgaccgggaa agaagacctg ccgattccgg aaaaaacggc
     2521 aactgcagaa atcaaaccga aacccgaaga agtcaaaccg aaaccgaaca tag
//
LOCUS       DAOQGJ010000383         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25573_length_2566_cov_3.414974, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000383 DAOQGJ010000000
VERSION     DAOQGJ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25573_length_2566_cov_3.414974"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PK683_07225"
     CDS             complement(<1..1234)
                     /locus_tag="PK683_07225"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNH08277.1"
                     /translation="MKLAEAAEYLAEKAKKAGVTEFDILGSSSESFGLEVFEKNISNT
                     EISIGTGIGIRLFSSKKMGLSYTERFTKESIDQCLKDAVSNSEITDILDLDLPGRQPI
                     PDVDLKTYSEDLEKIEFNDLADLCFRMEKAADGKDQRIENIPYIGSGKSKSEYIFLNS
                     NDVKYHLQYNSVSAYIGITSKENSQKKMGMYSNAYRKFSEFNPDFFASKAVERSVELL
                     GAKPVASAEYPVIISNRVSASLIGMFLSPYFAESVQKKQSRLSGRLNEKIGSPVVSLV
                     SRPHLPEMPGSRLLDGEGVPTKDITLVEKGVLKNFLYHLESAKKDGVAPTGSGQRSYS
                     GKAYTSVSNLFFEKGNESLESLMKKYPKCILITKLEGSSGCSALSGDISIGVQGFLVE
                     NGERVHPVDGITMNTNYFD"
     gene            complement(1231..>2566)
                     /locus_tag="PK683_07230"
     CDS             complement(1231..>2566)
                     /locus_tag="PK683_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNH08278.1"
                     /translation="DYTEIFVEETRSSSVSLSDGKVETASAGTEFGIGLRLISGTEVL
                     YASTSREDEDHLISLLVSLSKLRPDIQKITKSGNVFHLKDMSSPRQFPIAIDPRTVSQ
                     QRKLSLLSAADRKARSVSEKIAQVNARAFDSVSDIQIFNSEGVAVNDRRVYSRFTVSV
                     TAKKDSEVYSGSESPGAQRGFEFFESLDVEKNAETAAERALLMLGAAYIQGKKMPVVM
                     GSGFGGVIFHEACGHPLETEAIRKKSSPFVGKLGERIAQDCLTAVDDGTIENSWGSIQ
                     IDDEGMLPEKTVLIENGILKNYMSDRVGAAEVGVRRTGSSRRESYNYSPVSRMRNTYI
                     AAGKSTVDEMISSVTEGLYAKKMGGGSVNPATGEFNFAVEEGYIIRNGKIAEPVRGAT
                     LIGKGHEILPEISMTGNDLEISAGMCGASSGSIPVTVGQPTLKVDNILVGGR"
BASE COUNT          633 a          635 c          474 g          824 t
ORIGIN      
        1 gatcaaaata attcgtattc atcgtgattc cgtctaccgg atgcaccctc tcaccgtttt
       61 ccacaagaaa tccctgcact ccgatagaga tgtctcctga aagagcagaa cagcctgagc
      121 tcccttcaag ttttgtaatc agaatgcatt tgggatattt tttcattaaa gattccaggc
      181 tttcatttcc cttttcgaag aaaagattgg atacgcttgt atatgccttt cctgaatatg
      241 atctctgccc gcttcctgta ggagctactc cgtccttttt ggcagattcc agatgataca
      301 gaaagttttt cagcacaccc ttctccacaa gtgtaatgtc ttttgtagga actccttccc
      361 cgtccaaaag ccttgacccg ggcatttcag gaagatgggg tcttgaaaca agacttacaa
      421 cgggagatcc tattttttca ttcagtctgc ctgaaagtct ggactgtttt ttctgcacgc
      481 tttccgcaaa gtaaggagaa agaaacattc ctatcagaga cgcactgaca cggttggaaa
      541 tgatcacagg gtattctgct gaagctaccg gttttgctcc aagaagttcc actgatcttt
      601 ccactgcctt ggatgcaaaa aaatcaggat tgaattcaga gaactttctg tatgcatttg
      661 aatacattcc catttttttc tggctgttct cttttgatgt aattcctatg tatgcactta
      721 ctgaattgta ctgaagatgg tactttacat cattagaatt caggaaaata tattctgact
      781 tgctttttcc agatcctatg taaggtatat tttctattct ttgatctttt ccgtctgccg
      841 ctttttccat cctgaagcag agatccgcca gatcattgaa ctcaatcttt tcgagatcct
      901 ctgaataagt cttcagatcg acgtcaggga tcggctgacg tcccggaagg tccagatcca
      961 gtatgtctgt aatttcggag ttggatactg cgtctttcag acactggtct atagactcct
     1021 tagtgaatct ttccgtatag gacagtccca tctttttaga actgaacagt ctgatgccta
     1081 ttccggttcc tatgctgatc tcagtattgg aaatgttttt ttcaaacact tcgagaccga
     1141 atgactctga ggaactcccc agaatgtcaa attcagtcac tcctgctttt tttgcttttt
     1201 ctgcaagata ctccgctgct tctgcaagtt tcatcttccg cctaccagta tattgtccac
     1261 tttcaaagtc ggctgcccga ccgttacagg aatgcttccg gaagaagcac cgcacattcc
     1321 ggctgagatt tccagatcat ttcctgtcat tgaaatctcc ggaaggatct catgtccttt
     1381 tccaatgaga gtcgctcctc ttacaggttc tgcaatttta ccgttccgaa tgatgtagcc
     1441 ttcttccact gcaaaattga attcacctgt ggcaggattg actgaacctc ctcccatttt
     1501 ttttgcataa agaccttccg ttacagaact gatcatctca tcaacagtgc ttttgcctgc
     1561 ggcaatgtat gtgtttctca ttctggaaac aggggaataa ttgtaggact ctcttctgga
     1621 actgcctgtc cttctgactc cgacttcggc tgcacccacc ctgtcagaca tataattctt
     1681 taatatccca ttctctatta aaactgtttt ttcaggaagc attccttcat catcaatctg
     1741 aatggaaccc caggaatttt ctattgtccc gtcgtccact gcagtcagac agtcctgagc
     1801 aatcctctct ccaagttttc cgacaaacgg agatgatttc tttctgattg cttccgtttc
     1861 caaaggatgt ccgcatgctt catgaaagat gactccgccg aatccgcttc ccatcactac
     1921 aggcattttc tttccctgaa tgtaggctgc acccagcatc agaagagccc gctctgcagc
     1981 tgtctctgca tttttttcca catccagact ttcaaaaaat tcgaatcctc tctgtgctcc
     2041 tggagactct gagccggaat atacttcact gtcttttttg gcagttacac ttactgtaaa
     2101 tctggaatag actcttctgt catttacagc gactccttcc gaattaaaga tctgaatatc
     2161 cgaaacactg tcaaatgctc tggcatttac ctgtgctatc ttttctgaaa ccgatcttgc
     2221 ctttctgtcg gctgcactca ataaactgag ttttcgctgc tgactgactg tccttggatc
     2281 aattgcaatc ggaaactgtc ttggactcga catatccttc agatgaaata catttcctga
     2341 ttttgtaatt ttttgtatgt cgggacgaag cttggaaaga ctgacaagaa gagaaatcag
     2401 atgatcttca tcttccctgc tggtacttgc atacagaacc tctgtgccgg aaatcagcct
     2461 gagcccgatc ccaaattcag ttcctgcact tgcagtttcc acttttccgt cagaaagact
     2521 gacagatgaa gaacgagttt cttccacaaa gatttctgta taatca
//
LOCUS       DAOQGJ010000384         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25663_length_2560_cov_2.778044, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000384 DAOQGJ010000000
VERSION     DAOQGJ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25663_length_2560_cov_2.778044"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PK683_07235"
     CDS             complement(<1..1062)
                     /locus_tag="PK683_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HNH08279.1"
                     /translation="MIQLKIKKEHLPETVKCFQKWKPSWTIYAVHNDNIYDLLRENAG
                     IWTEAESIRIAGNKYSLKEFSGNLHFRVKAGDGKSFIFILKEKEYILLVNDETDFSSA
                     PALPRIYWNRNKKRGSLNVLKKFSETQDLIVDECLGVKNLNALSRMKNLKNLSLTFCR
                     QLENIQGIADLASLQNLYIHFCFLLEDISPLSNLTELETLTIAYAYSMRNTDSLSSLK
                     NLKSLDLYGANLTDIEGLRTLSGLRSLCLGYCMTLENFEPLSRLKGLTDLDLTNCLGL
                     TDASFLSDLTELRSLSLNSCGNLPDISPISKLTELKILDLSWCVEIQDIRALKKLQNL
                     TELRMNGTHLKDLNVFSFLP"
     gene            complement(1291..2472)
                     /locus_tag="PK683_07240"
     CDS             complement(1291..2472)
                     /locus_tag="PK683_07240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH08280.1"
                     /translation="MNGSFVFHIPTLFVIMAGITFITTFLLVIASLSSDKNRELKLWS
                     QGNVSICLGFLIGGAPNVPDFVHAMMSYGLMGLGLGYVYAGLRVFIGKKYSWTSVLSI
                     AVIAGLSSGYYVYLKPSLSGRLLISSLYFGMLNLLCAFTLLRPSFAIEKGALWLAGSG
                     FAVMSIALFCRAAFILYQEQMSSFVSNQVEAVTLLLTAMVQIWIAFSLIFMITSRYAK
                     EILHLSMTDTLTGILNRSGLNSLGMRMLLRAEQKKTPMAVLVFDADHFKKINDRYGHL
                     FGDEILRLLAQFSQNALRPNDLLARFGGEEFIAILDSVSQEDSILIAERLCRTIEKSD
                     FKVEDTQIPCTVSIGVSCTGLSDTYSLQALISSADQALYRAKQEGRNRVCLSESKIPS
                     Q"
BASE COUNT          731 a          530 c          524 g          775 t
ORIGIN      
        1 aggaagaaag gaaaatacat tcaagtcttt cagatgagtg ccgttcattc tgagctctgt
       61 caggttctga agttttttta atgctcttat atcctgaatt tcgacgcacc agctcaaatc
      121 cagtatcttc agctcggtaa gcttcgaaat tggactgata tccgggagat ttccgcagct
      181 gttcagactg agagaacgaa gttcggtcag atcagaaaga aaagaggcat cggtcagccc
      241 aaggcagttt gtgagatcca gatcagtcag tcctttcagt cgggaaagcg gttcgaaatt
      301 ttcgagagtc atacagtatc ccagacagag agacctcagt ccggaaagag ttctgagtcc
      361 ttcaatgtca gtcaggttgg ctccgtacaa atccaatgat tttagatttt tcagacttga
      421 gagagaatca gtgtttctca tactgtaagc gtatgcgatt gtaagtgttt ccagttcggt
      481 caggtttgac aggggactga tatcttcaag aagaaaacag aaatgaatgt aaagattttg
      541 aagagatgcg agatccgcaa ttccttgaat attttccagc tgtctgcaga aggtcaaact
      601 gaggttcttc agattcttca ttctagaaag agcattcagg ttttttacac ccagacattc
      661 atctacaatt agatcctgtg tttcactgaa ttttttcaga acattcagac ttcctctttt
      721 tttgtttctg ttccagtaaa tgcgcgggag ggcgggagca gaactgaaat ctgtttcatc
      781 attgacgagc agaatgtatt ctttctcttt caggataaag ataaaacttt ttccatctcc
      841 tgcttttact ctaaagtgca gattaccgga aaattccttc agactgtatt tatttcctgc
      901 aatgcggatg ctttctgctt cagtccagat ccctgcattt tccctcagaa gatcatatat
      961 attatcatta tgaactgcat atattgtcca gctcggtttc catttctgaa aacatttgac
     1021 tgtttccggg agatgttctt tttttatttt cagttgtatc attgcagaga tttgactgca
     1081 cagttcagca tttcatatat ccgaatagac ttctatgctt tgagcagtgc tgattccttt
     1141 cgatacagta attcaagtta ttttatttcc gcaactttat tttaggaaac tgatatgagg
     1201 ataaggagtt taccttacgc aataggcttt catatattca gtattcagaa gagtctttgg
     1261 tcatctgatt cattgcagac agctcatcca ttactgtgaa ggaatctttg attcgctcag
     1321 gcatactctg tttcttcctt cctgtttagc acggtacagt gcctgatcag cggaactgat
     1381 gagagcctgc aggctgtatg tgtctgacag acctgtacag gataccccga tgctgactgt
     1441 gcagggaatt tgagtgtctt ccactttgaa gtctgatttt tcaatggttc tgcaaagtct
     1501 ttctgcaatt aatatggaat cctcctgact gactgaatcc agtattgcaa taaattcttc
     1561 tccgccgaat cgggccaata gatcattggg tctcagagcg ttttgagaaa actgcgcaag
     1621 cagtctgagt atttcatctc cgaataaatg accgtatctg tcgttgatct ttttgaaatg
     1681 atctgcatca aaaacgagaa cagccatcgg agtttttttc tgctctgccc tcagaagcat
     1741 acgcattccc agactgttca atccgctccg gttcagaatt ccagtaagag tgtcagtcat
     1801 ggaaagatgc agtatctctt tcgcataacg gcttgtgatc ataaatataa gagaaaaggc
     1861 aatccagatc tgcaccattg cagtcagcag cagagttact gcttccacct gatttgatac
     1921 aaaggaactc atctgttcct gatacagaat aaacgcggct cggcagaaca aggcaatgct
     1981 catcactgca aatcctgaac cggcaagcca aagagcgcct ttctcaattg cgaaggaagg
     2041 ccgaagtaat gtaaatgcac agagtaaatt aagcattcca aaataaagag aggaaattaa
     2101 cagtctgccg gaaagactcg gtttcaagta tacgtaatag cctgaactca gccctgcaat
     2161 gactgcaatg ctcagtactg aggtccacga atattttttt ccgatgaata cccgcaggcc
     2221 tgcatacaca tatcctaagc ccagacccat cagcccgtag ctcatcattg catgtacaaa
     2281 atcaggcaca tttggggcac cgccgattaa aaagccgaga cagatggaaa catttccctg
     2341 agaccagagt ttcagttctc tgtttttatc ggaagataaa ctggcaatta caagcaggaa
     2401 tgtggtgata aaagtgatgc ctgccattat gacaaataag gtaggtatat gaaatacaaa
     2461 ggatccattc atctattgtt ccgagtaggt tattttatat aagcacttaa aaattcatat
     2521 actttattta ttttaaggat ttcagcattc aataatgctt
//
LOCUS       DAOQGJ010000385         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25680_length_2559_cov_2.971645, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000385 DAOQGJ010000000
VERSION     DAOQGJ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25680_length_2559_cov_2.971645"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /gene="thiH"
                     /locus_tag="PK683_07245"
     CDS             complement(<1..1078)
                     /gene="thiH"
                     /locus_tag="PK683_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNH08281.1"
                     /translation="MFTEIFENYDFRTESEKIKNRTIEDVEQALSNALQGRMLFDDYL
                     SLLSPSADRLLTEMAELARRITRQRFGNIMQIYMPLYLSNECRSSCLYCGYSFENKLP
                     RITLAKAELTREAEIIHSKGIRQVLALTGEDYSRTPVSYIADSVSLLNSYFPSVSIEI
                     YPMDTDDYSKIINAGAEGLALYQETYHPENYKTYHIRGMKKNMKYRLEGPDRGGTAGF
                     RRMSIGALLGLSDSLGEMFFLGLHAEYLQKKYWKSLIQVSLPRLKHAEGNFSKEYNVT
                     DREFLRFIFALRIYFNDLGITLSTRERPELRDAVIQYGITSVSAESKTEPGGYQGIEA
                     LEQFETEDKRTVSEIKDMIRRSGLD"
     gene            complement(1078..1866)
                     /locus_tag="PK683_07250"
     CDS             complement(1078..1866)
                     /locus_tag="PK683_07250"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNH08282.1"
                     /translation="MTEDLLRLGGRTFRSRLFLGTGKFSSSSVMKKAIQSSGTEMVTA
                     ALRRVDLENPEDDILSHIDRNSVVLLPNTSGARNAEEAVRVARIARSLGAGEWLKLEV
                     TPDPVFLLPDPIETLKAAQILVKEGFKVMPYINADPILCRHLEDAGCISVMPLGSPIG
                     SNQGIRTKANLEIIIHSSNVPVVVDAGLGEPSHAAEAMEMGASAVLVNTAIAAAKDPV
                     QIANAFRLATEAGRLSFLYRGAGRSSISELKADPSSPLKNILGS"
     gene            complement(1863..>2559)
                     /locus_tag="PK683_07255"
     CDS             complement(1863..>2559)
                     /locus_tag="PK683_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08283.1"
                     /translation="MTLKQDAEESKMREIKSEIQILGSINPLAIEEFRNVKEIYDHHK
                     SQRDDIEKSKADIEKVIREINAESEKLFIETFEIIQGNFQDTFSTLFNGGSARLVLSD
                     RTDPLNSGIEIIAEPPGKHVQNLKLLSGGEKSMTVIALLFAIYMVRPSPFCFLDEIDA
                     ALDEVNKIRFCQILDKFKDKSQFVVITHAPPTISRASTIFGVTAEEPGVSKLLSLKLE
                     EAKNFSRKVQKAV"
BASE COUNT          618 a          595 c          557 g          789 t
ORIGIN      
        1 gatcgagtcc cgatcttctg atcatatctt tgatttccga gactgttctt ttgtcttctg
       61 tttcgaactg ttcgagtgct tcgattccct ggtatcctcc gggttctgtt ttggattctg
      121 cagaaacgga agtgatcccg tactgtatga ctgcatctct gagttcaggt ctttctctgg
      181 tggaaagtgt aatgcctaaa tcattaaaat atattcttag tgcaaatatg aatctcagaa
      241 attccctgtc agtgacattg tattcctttg aaaaatttcc ttctgcatgt ttcagacggg
      301 gcagggaaac ctgaatcagg cttttccagt attttttctg aagatattct gcgtgcagtc
      361 cgagaaagaa catttctccc agactgtccg aaagtccaag aagtgctcct atggacattc
      421 ttctgaatcc tgctgtcccg cccctgtccg gtccttccag gcggtatttc atattctttt
      481 tcatcccgcg aatatggtat gttttataat tttccggatg atatgtttcc tgataaagtg
      541 caagtccttc tgcacctgca ttgatgattt tagagtagtc atccgtatcc atgggataga
      601 tctctatgga tacagacggg aaataggaat tcagaagact gactgagtct gcaatatagg
      661 aaacaggggt tctggaatag tcttctcctg taagagcaag aacctgcctg atccctttgg
      721 aatgtatgat ttctgcttcc cttgtaagct ccgcttttgc aagagtgatc ctcggaagtt
      781 tgttttcaaa actgtatccg cagtacagac aggaggatct gcattcattc gaaagataca
      841 gaggcatata aatctgcata atgtttccga atctctgcct cgtgattctt cttgcaagtt
      901 ccgccatttc agtcaggagt ctgtctgcag aaggagagag gagagacagg tagtcgtcaa
      961 agagcatcct tccctgaaga gcattggaca gagcctgttc tacatcttcg attgtacggt
     1021 tttttatctt ttccgactct gtgcggaaat cataattctc aaatatctca gtaaacatca
     1081 gcttcctaga atgttcttca gaggactgga aggatctgcc ttgagctctg agatactgct
     1141 tcttccggca ccgcggtaca gaaaggaaag cctgcctgcc tctgtggcaa gtctgaatgc
     1201 atttgcaatc tggacaggat cctttgctgc agcaatcgct gtatttacta aaactgcaga
     1261 agctcccatc tccattgctt ctgctgcatg actaggctcc ccgagacctg catccactac
     1321 aacgggcaca ttcgaagaat gaatgatgat ttccaggttt gcctttgtcc gaattccttg
     1381 attggaaccg atcggagaac cgagaggcat tacagatatg caccctgcat cttcgagatg
     1441 tctgcatagg atcggatctg cattgatgta gggcattacc ttgaatccct ctttgaccag
     1501 gatctgtgct gctttcagtg tttcaatcgg atcggggaga aggaaaacag gatcaggagt
     1561 gacttcgagc ttcagccatt cgcctgcacc tagagatctc gcaattcttg ccaccctgac
     1621 tgcttcctct gcatttctcg caccgcttgt attcggaaga agcacaactg agttcctgtc
     1681 tatatgcgag agaatatcgt cttcaggatt ttcgagatcc actcttcgga gggccgctgt
     1741 caccatctca gttcctgagg actgtatagc ttttttcatt acagaagagg aggaaaattt
     1801 tcctgttccg agaaacagcc ttgaacggaa tgttcttcct ccgagacgga gcagatcctc
     1861 tgtcatacag ccttctgtac cttccttgag aaattttttg cctcttccag tttcagtgac
     1921 agaagttttg aaaccccagg ctcttctgcc gtcactccga agattgtgct cgcacgggag
     1981 atggtaggcg gtgcgtgagt gatgactacg aactgactct tatccttgaa cttgtccagt
     2041 atctgacaga accggatctt gttcacttca tcaagagcag catctatctc gtccaggaaa
     2101 cagaaagggg agggtctgac catataaatt gcaaacagaa gcgcgatcac tgtcatagac
     2161 ttttcaccgc cggaaagaag cttcagattc tgcacatgtt ttccaggagg ttctgcaatg
     2221 atttcaattc ctgaattcag cggatctgtt ctgtccgaga gcacaagtct tgcacttccc
     2281 ccgttgaaaa gtgtggagaa tgtatcctga aaattgccct gaatgatttc aaatgtttca
     2341 atgaaaagtt tttcagactc cgcattgatt tcacggatga ctttttcaat gtccgccttg
     2401 ctcttttcta tatcgtctct ctggctttta tgatgatcgt agatttcctt tacatttctg
     2461 aattcttcga ttgcaagagg attgatcgaa cctagaatct gtatttccga tttgatctcc
     2521 ctcattttgg actcttcagc atcctgcttc agtgtcatc
//
LOCUS       DAOQGJ010000386         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25749_length_2554_cov_3.430972, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000386 DAOQGJ010000000
VERSION     DAOQGJ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25749_length_2554_cov_3.430972"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PK683_07260"
     CDS             <1..324
                     /locus_tag="PK683_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08284.1"
                     /translation="DEGLSLRNPVVRENQKDIDLTAGKKTLKILKVEGDTVIINAGAV
                     HGIRHGYVGYIAEQENYETYSYLAQFVVENVFDKSSKCTITEGRGKLKRDMNEGLKEF
                     DVVLK"
     gene            321..1559
                     /locus_tag="PK683_07265"
     CDS             321..1559
                     /locus_tag="PK683_07265"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH08285.1"
                     /translation="MKRIIEYILSALLIIFAVSLFSEPRKKGLRIESSDGKRIRRWAV
                     AAGINSWHDVSISKLNKAQNDAKEFSRLLKEKGEFDEIITLTDDNDPRSSLYPSRNNI
                     LTKLELLLDNAEPEDLFIFYFSGHGISDKDDNAYLMPADAVFEKPLETAVSVEYIISL
                     FKKKNMKKTLLIVDACRETTAKVKGTGQNSIKAEKYGDTEIGAVFFSTGSGNYSYEDH
                     ESENGVFTRFLKEGISGAADADQDGIVTFSELENFVQEKVRGWSLKFNKKQRPYVRFS
                     REKSGEIPISAVSAASDSSEKTLKPSEIQYNDKRQEVSAQGLFVLNGKKWKTSGQKSG
                     WARAFSICAESEMKLPSAEELNSLYRSKAEWKKQECSSPCIYWTRDHNDFNAYTVSME
                     DGMKTTAQKILNRSSICIEK"
     gene            complement(1563..>2554)
                     /locus_tag="PK683_07270"
     CDS             complement(1563..>2554)
                     /locus_tag="PK683_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08286.1"
                     /translation="DESVSSLEKAGKLLIENGERNTSAYLRVQYYTALSLYFKGEYNI
                     ALGQFLKAKAGAESLGQTDLMYLQLNDFIADTYMRKGQFKSAAACFLDIKRRYEMMNF
                     TDSLLYANVLNNLALHYQDTFSETSLEYLKRAEEHYRKNGYEDKAGYPNTLSNIGNYF
                     LEKKDTENALNYYRSASEFYERYGHTLTFGYANTLQNTAGIMMMKKDFDGSLIRFEKS
                     REVYERILSASSWQYAMTVNRIADIQLIQGNRLKAEENSRLAREIYQKLGLMESHLYG
                     YALETAGDIRKVNGDLKISADFYDKAYSVYQKNRKTDEARSVFMKYKAAKGDI"
BASE COUNT          812 a          518 c          526 g          698 t
ORIGIN      
        1 gacgaaggac tgtccctcag aaatcctgta gtgagagaga accagaagga tattgatctg
       61 actgccggta aaaagacttt gaagattctg aaagtggaag gagacactgt cattatcaat
      121 gccggtgcag ttcacggcat cagacatgga tacgtcggct acatcgcaga acaggaaaac
      181 tacgaaacat attcttacct ggcacagttc gttgtagaaa acgtatttga caaatcatcg
      241 aaatgcacca ttacggaagg cagaggaaaa ctgaaaagag atatgaatga gggactgaag
      301 gagttcgatg tggttctgaa atgaagcgca tcattgaata tattttatcg gcactgctta
      361 ttatattcgc agtttcactc ttttcagaac cccgaaaaaa gggtctgcga atagaatcct
      421 ctgacggaaa acggattcga cgatgggctg tcgcagcggg aataaacagc tggcatgatg
      481 tatcaatttc gaaactgaac aaggctcaga atgatgccaa agagttttca agacttctga
      541 aagagaaggg agaatttgat gaaatcatta ctctgactga tgacaatgac ccaagatcat
      601 cgctttatcc ttcaagaaac aatatactga caaagcttga gcttcttctg gataatgctg
      661 aaccggaaga tcttttcatt ttttatttct ccggacatgg aatctccgac aaggacgaca
      721 acgcatatct gatgcccgcc gatgccgtct ttgaaaaacc gctcgaaaca gcagtatcag
      781 tagaatatat catatctctt tttaaaaaga aaaacatgaa aaaaaccctt ctcattgtcg
      841 atgcctgcag agaaaccaca gcaaaagtaa aaggtactgg acagaattca attaaagctg
      901 aaaaatacgg tgatacggaa ataggagcag tcttcttttc cactggatca gggaactaca
      961 gttacgagga tcatgaatca gaaaacggag tctttaccag atttcttaaa gagggaatat
     1021 caggagccgc tgatgccgat caggatggga ttgtcacctt ttcagaatta gaaaactttg
     1081 tacaggaaaa agtaagaggc tggtcactga aattcaacaa gaaacagaga ccctatgtac
     1141 gattcagcag ggaaaaatca ggcgaaattc cgatttctgc cgtctcagcg gcttcagatt
     1201 catcagaaaa aacactgaag ccttcggaaa tacaatataa tgataaaagg caggaagtat
     1261 ccgctcaggg gctttttgtc ctgaacggaa aaaaatggaa aacatcaggg caaaaatccg
     1321 gctgggcacg tgccttcagc atatgtgcag agtcggaaat gaagctcccg tcagcagaag
     1381 aactgaattc tctttacaga tccaaggccg aatggaaaaa acaggaatgc tcaagcccgt
     1441 gcatatactg gacaagagac cataatgact ttaatgcata tacagtttcc atggaagacg
     1501 gaatgaagac aactgctcaa aaaattctga acagaagttc aatctgcatt gaaaaataat
     1561 tactaaatat ctcctttcgc tgctttgtac ttcataaaaa cagatctggc ttcatctgtc
     1621 tttcgatttt tctggtagac agagtatgct ttgtcataaa aatcagcaga tattttaaga
     1681 tctccgttca ccttcctaat atcaccggct gtttcaagag catatccgta caaatgagat
     1741 tccatcagac caagtttctg ataaatttcc ctggcaagtc tggaattttc ctctgctttc
     1801 agcctgttcc cctgaataag ctgaatgtct gcaatccggt taactgtcat tgcatactgc
     1861 catgaagaag cagaaagaat tctttcgtac acctctcttg atttttcaaa tcgtatgaga
     1921 cttccgtcaa agtctttttt catcatcatt atcccggctg tattctgaag cgtgttggca
     1981 tacccgaaag tcagagtatg tccatatctt tcgtagaatt cagatgcaga tctgtagtaa
     2041 ttcagcgcat tttccgtgtc ttttttttcc agaaaataat tgcctatatt gctgagggtg
     2101 ttcggatacc ctgctttgtc ttcatagccg ttttttctgt aatgctcctc tgctcgtttg
     2161 agatattcca gagaggtttc cgaaaaggta tcctgatagt gaagtgcaag attgttgagc
     2221 acatttgcat agagcagaga atcagtgaaa ttcatcattt cataacgtct ctttatgtcg
     2281 agaaagcagg ccgctgcaga cttgaactgt ccctttctca tatatgtatc tgcaataaaa
     2341 tcattcagct gaaggtacat taagtctgtc tgacccagcg attctgcacc agcctttgcc
     2401 tttaaaaact gtccgagagc aatattgtat tctcctttaa aataaagaga aagagcagta
     2461 taatactgaa ccctcagata tgcagatgta ttcctttctc cattttcgat cagaagtttt
     2521 cctgcttttt cgagggaaga aacagattca tcca
//
LOCUS       DAOQGJ010000387         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25785_length_2552_cov_3.541850, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000387 DAOQGJ010000000
VERSION     DAOQGJ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25785_length_2552_cov_3.541850"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1088
                     /gene="coxB"
                     /locus_tag="PK683_07275"
     CDS             147..1088
                     /gene="coxB"
                     /locus_tag="PK683_07275"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004470589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNH08287.1"
                     /translation="MPFQGTGIAGEVDNLYMFLLVSSLISFIILIGGMTYYILRFKRK
                     TNEDKTLYITHNTFLEFLWSFIPFVIMMVIFFWGKVIYDKVRHIPEAGEEIQVNALQW
                     AWQVKYKNGIRLNSKTAGQDVMHIPVNKPVKIILTASDVLHSFYIPAFRIKQDAIPGR
                     VVYLGFTADKPGLYQVFCAEYCGVDHSNMPLKIKAESQAEYDAWLANNVPKEAAGGNA
                     LAAKGATVYNEKTCVGCHSLDGSPRAGPTWKGLYEAKREFADGTTGQANDAYLRESIL
                     EPGKKIVKGFGPVMPSFKGQLDDEEIQAVIEYMKSLK"
     gene            1098..>2552
                     /locus_tag="PK683_07280"
     CDS             1098..>2552
                     /locus_tag="PK683_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNH08288.1"
                     /translation="MSANAHTAENDNYLQHGKGLGSWLYTLDHKRIAILYFIAVLTFF
                     LIGGIFALLVRTELFSPGKTIVEAATYNRFMTMHGAIMVFMVIIPGIPAIFGNFLLPI
                     MLGAKDVAFPRLNMYSWYVFMLGALIAASTLITGGVDSGWTFYTPYSITTQTSVVVMV
                     LGAFTMGMSSIMTGLNFVVTVHTLRAPGQTMSRIPLFVWAIYATAIIQILATPVLGIT
                     LLLIAAERVLGVGIFDPKLGGDPVLFQHFFWFYSHPAVYIMVLPAMGIISELVSTFSR
                     KTIFGYTAIAYSSLAIALVSFLVWGHHMFVSGQSEFAGLLFSVLTMLVGVPTAIKLFN
                     WIATMYGGSIRLDTPMLYAIGFMFLFTIGGLTGVFLANVGTDMHFHDTYFVVAHFHYV
                     MVGGTLMAFTGGIFYWFPKMFGKMISDSLGRVSWAFVFTGFNVTFFPQFVLGAMGMPR
                     RYYDYLPEYTSLNQISTVGAFLIAIGFVIALVAII"
BASE COUNT          653 a          520 c          595 g          784 t
ORIGIN      
        1 atgagactcg gggcagtgct gaccgtactc cttttaggcg tgtatatatt tactttctgg
       61 aaaagtacac aaaaaaaatc tgattttcaa tctacggggg gagtgtaatg ttctcttggc
      121 ttcaggtaac tttggctgac acttttatgc cttttcaagg aacgggcata gcaggtgaag
      181 tcgacaacct atatatgttc ctgctggtgt ccagtctgat ttcgttcatt attcttattg
      241 gtggaatgac ttattacatt ctccgcttta aaagaaaaac gaatgaagat aagactttgt
      301 atatcactca caatactttt ctcgagtttc tgtggagttt tattccgttc gtcatcatga
      361 tggtgatctt cttttgggga aaagtgattt acgataaagt aagacatatc cctgaggcag
      421 gtgaggaaat tcaagtgaat gcccttcagt gggcttggca ggtgaaatac aaaaacggaa
      481 ttcgtctgaa ttcaaagact gccggtcagg atgtaatgca tattcctgta aataagccgg
      541 tgaaaatcat cctgactgca agtgatgttc ttcacagttt ttatattcct gcattcagaa
      601 tcaaacagga tgcgattcct ggaagagtgg tttatttagg ctttactgca gacaaaccgg
      661 gactgtatca ggtgttctgt gcagaatact gcggtgtaga ccactccaat atgcctctga
      721 aaattaaggc agaatctcag gcggaatatg atgcctggct tgcaaacaat gttcctaagg
      781 aagcagctgg cggaaatgct cttgctgcca aaggagcgac tgtttacaac gagaaaacct
      841 gcgtaggatg tcattcactc gacggatctc ctcgggccgg ccctacatgg aaaggtcttt
      901 atgaagcaaa gagagaattt gcggacggaa caaccggaca ggcgaacgat gcttacctca
      961 gagagtcaat tcttgagcct ggaaagaaaa tagtaaaagg attcggtcct gtaatgcctt
     1021 cattcaaagg tcaactcgat gatgaagaga ttcaggctgt aatcgaatat atgaaatcat
     1081 tgaaataagg ggactttatg tcagcgaatg cgcatactgc cgaaaacgat aactatctgc
     1141 aacatggcaa aggactaggt tcttggcttt atacattgga tcataaaaga attgcaatcc
     1201 tgtattttat tgcagttctt acgttttttc tcattggggg aatttttgct cttctcgtaa
     1261 gaacagaact gttttctcct ggaaaaacaa ttgtagaagc cgcaacctat aaccgtttta
     1321 tgacaatgca cggagcaatc atggtattta tggtgattat tccgggtatc cctgccatct
     1381 ttggaaactt cctgctgcct attatgctgg gagcaaaaga cgtggcattc cccagactga
     1441 atatgtacag ctggtacgtg tttatgctcg gagccctcat tgccgcttct actctgataa
     1501 ctggcggggt ggattccgga tggacattct atactcctta cagtattact acccagacaa
     1561 gtgtggttgt gatggtgctg ggtgctttca cgatggggat gtcttccatt atgactggac
     1621 tgaactttgt agtgactgtt catacgctca gagcacctgg tcagacaatg tccagaattc
     1681 ctcttttcgt atgggcaatc tacgcaacag caatcatcca aatcctggca actcctgttc
     1741 ttgggatcac acttctgctg attgcagcag aacgagtgct cggagtcggt atatttgacc
     1801 cgaaactcgg aggagatcct gttcttttcc agcacttctt ctggttctat tcacaccctg
     1861 cagtatacat tatggttctt ccagcaatgg ggatcatcag tgaactcgta tctacattct
     1921 ccagaaaaac gattttcggt tacactgcaa ttgcctactc cagtcttgcg attgcacttg
     1981 tgagctttct ggtctgggga catcatatgt tcgtgagcgg tcagtctgaa tttgcaggcc
     2041 ttctgttttc agtgcttaca atgctggtcg gagttcctac tgcgattaag cttttcaact
     2101 ggattgccac gatgtacgga ggatcgattc gactggatac accgatgctc tatgcgatcg
     2161 gatttatgtt tctgtttacg attggcggtc tcaccggagt atttctggcg aacgtaggaa
     2221 cagatatgca tttccatgat acttatttcg ttgttgcaca ctttcactat gtgatggtgg
     2281 gcggaactct gatggctttc acggggggga ttttctactg gtttcctaaa atgttcggaa
     2341 agatgatcag tgattctctg ggaagagttt cctgggcatt tgtatttacc ggttttaatg
     2401 tcactttctt tcctcagttt gttctgggtg caatgggtat gccaagaaga tactatgatt
     2461 atcttcctga atacacaagt ctgaatcaga tttccactgt cggtgcattc ctgattgcaa
     2521 tcggttttgt gattgcactg gtcgcaatta tc
//
LOCUS       DAOQGJ010000388         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25837_length_2549_cov_3.378909, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000388 DAOQGJ010000000
VERSION     DAOQGJ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25837_length_2549_cov_3.378909"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PK683_07285"
     CDS             <1..902
                     /locus_tag="PK683_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNH08289.1"
                     /translation="FIQVKDEAVSSFISDPENWILGQGTIYPDTIESGGTKHSHNIKT
                     HHNRSQAVLDLLEKGLVVEPLKELYKDEVRELGSLLGLSMDIIERHPFPGPGLAVRMI
                     ASAEDNEFEDREQSDRLIAHYKDAQYKILPLKSVGVQGDSRSYRHCALLNDFTRSWHE
                     YRKVSTDITNHIRGINRVVFAPFQRNLPDNLKFSQIFLDKSHSDLLRDADFIVSKILK
                     KRGISQEIWQMPVVLIPVGKKKGRFSIVLRPVESMEAMTANFYPMRRKFLKEIVKSLK
                     KLKKISWVFYDITDKPPGTIEWE"
     gene            903..1625
                     /locus_tag="PK683_07290"
     CDS             903..1625
                     /locus_tag="PK683_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNH08290.1"
                     /translation="MMNEPEKKQKKTIFEDEVFTKKQIMEFSKVVKVIHSYNDDKEDY
                     RWDLLSCFVQALFWTGCRISELVKVTYEDIEEIMKNDIFLKVHISGKRERSVLMPKSL
                     LTEIRLITKKEIGYLFQSRQGLPFKKDSVFRELGKIRRELTDRDIYTDIFKHSRAMHL
                     YRLTKDLKLIQKYLGNPRKNFYEFVFSEKPENRGKYVLQVFRKDKLVSIQRFETSEER
                     AEAKAELKALKNHYKFISGKDE"
     gene            1622..>2549
                     /locus_tag="PK683_07295"
     CDS             1622..>2549
                     /locus_tag="PK683_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08291.1"
                     /translation="MNTQEYISAYLQENIAESETLHRMKNVIAEFAARAPKILVTKCI
                     DGRVHGSKAKGYPATTIRFGRTDGNTVSTEKNNYWFWNRIDTTVNSALCNTPEMPAIF
                     IAYMHRSDLGYGCAAHNNNDEAAFHAVQNQTRAVKKAFPTGNLYVLEGIINTDTMAET
                     LIFDEKVRISSEEIIKDFNLNELKDIFFKGFLKYPIKDPASARLVHFKTPEELMQGDA
                     PLFHSDFQTSLSMKSFLLLEISRIIYEDDYDSQKVIQPDIFNAIISQLDKVKTLPRSL
                     RAPFFYQIFWNISYALYQKKKISSMTPEESIKL"
BASE COUNT          843 a          493 c          533 g          680 t
ORIGIN      
        1 tgtttatcca ggtaaaggat gaagctgttt cttcatttat ctctgatccg gaaaactgga
       61 ttcttgggca gggtacaatt tatccggata caatagaaag cggcgggaca aaacattctc
      121 ataatataaa aactcatcac aacagaagcc aggctgttct ggatcttctg gaaaaaggac
      181 ttgttgttga accgctgaaa gagctttaca aggatgaagt cagagagctg ggaagtctgc
      241 tcggactcag tatggatatc attgaaagac atcctttccc gggtccaggt cttgctgtaa
      301 ggatgattgc ttctgctgaa gacaatgaat ttgaagacag ggagcagtcg gacagactga
      361 ttgcccatta caaggatgcg caatataaaa tattgcctct gaagtcggta ggcgttcagg
      421 gagattccag aagctacagg cactgtgctc ttctcaatga ctttacaagg agctggcacg
      481 aataccgaaa agtgtctaca gacattacca atcatattcg cggaatcaac agagttgtat
      541 ttgctccttt ccaaagaaat cttccggaca atctcaaatt ttcacagatt ttcctggaca
      601 agtcacattc agatcttctc agagatgcag attttatcgt aagcaagatt ctgaaaaaaa
      661 gaggaatttc tcaggaaatc tggcagatgc cggttgttct gattcctgtc ggaaagaaaa
      721 aaggcagatt cagcatagtt ctaagaccgg tagagtcaat ggaagcaatg actgccaact
      781 tttatcctat gagaagaaag tttttgaaag aaatagtaaa atcacttaaa aagctcaaaa
      841 agatttcctg ggttttttat gatattaccg acaagccccc gggcacaatt gagtgggaat
      901 agatgatgaa tgaacctgaa aaaaagcaga aaaagaccat atttgaagat gaggtcttta
      961 caaaaaaaca gattatggag ttttccaaag tcgtcaaagt aattcattcc tacaatgacg
     1021 acaaggaaga ttaccgctgg gatcttcttt cctgttttgt tcaggccctt ttttggacag
     1081 gctgccgtat ttctgaactc gtaaaagtga catatgaaga tatagaagaa ataatgaaaa
     1141 atgatatttt tttgaaggtt catatttccg gaaaaagaga gaggtcagtc ctgatgccca
     1201 agagccttct gactgagatc cgtctgatta caaagaagga aataggttat ctttttcaga
     1261 gcagacaggg actccctttc aagaaagatt ctgtattcag agagcttgga aaaatccgaa
     1321 gagaactgac agacagggat atatatacgg atatcttcaa gcattcaagg gcaatgcatc
     1381 tttacagact gaccaaggat cttaagctga ttcagaaata tctcggcaat cctaggaaaa
     1441 atttttatga atttgtattt tcagaaaagc ctgaaaatcg cggaaaatat gttcttcagg
     1501 tctttcgtaa ggataaactt gtctcaattc aaagatttga aacgtcagaa gaaagagccg
     1561 aggccaaggc tgaacttaag gcattaaaaa atcattataa attcatcagc ggaaaggatg
     1621 aatgaacacg caggagtata tctctgcata tcttcaggag aatattgcag aatctgaaac
     1681 actgcacaga atgaaaaatg taatcgcaga atttgctgcc cgtgctccga agattctggt
     1741 aacaaaatgc atcgacggaa gggtccacgg gagcaaagca aaaggttatc ctgccactac
     1801 aatccgtttc ggaagaacgg atggaaatac cgtttccacc gaaaaaaaca attactggtt
     1861 ctggaaccga atcgatacta ctgtcaacag cgctctctgc aatacccctg aaatgccggc
     1921 aatattcatt gcttatatgc accgttccga tctgggatac ggatgtgctg cacacaataa
     1981 taatgatgaa gcggcttttc atgcagttca gaatcagacc cgcgcagtaa aaaaagcatt
     2041 cccgaccggc aatctgtacg tgcttgaagg catcataaat acagatacaa tggcagagac
     2101 gcttatattt gatgaaaaag tcaggatcag ttcagaagaa ataataaaag attttaatct
     2161 aaatgaactt aaggatattt tcttcaaggg atttctcaaa tacccgatca aagatcctgc
     2221 aagtgccaga cttgtgcact tcaagactcc tgaggaactg atgcagggtg atgcacctct
     2281 ttttcattct gatttccaga cttcactttc aatgaaatca tttcttcttc tggagatttc
     2341 acgtattatc tacgaggatg attatgattc tcagaaggtg attcagccgg atatttttaa
     2401 tgcaatcata tcccagctgg acaaagttaa gactcttccc agatctctcc gcgctccgtt
     2461 cttctatcag atcttctgga atatcagcta tgcactttat caaaaaaaga aaatctcctc
     2521 aatgacaccg gaagaaagca tcaagcttc
//
LOCUS       DAOQGJ010000389         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25838_length_2549_cov_3.271051, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000389 DAOQGJ010000000
VERSION     DAOQGJ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25838_length_2549_cov_3.271051"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1875
                     /locus_tag="PK683_07300"
     CDS             46..1875
                     /locus_tag="PK683_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016755576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNH08292.1"
                     /translation="MKGVLIRNLLGVFIICFCCEILSQETSKEKTKAKEAETEQPLSL
                     DPPKDIQYDAQPNDGKGIPEIQAELAKEEPYKSPYKGKLPGEFMRTMLLAPDHQDAVR
                     KVDRLWFADIIRTGFQLRPRFDYTHNLDFDKRTQDDRNFGTQNSQLWFVIDPHPNVAA
                     KITIQDVRVFGGAQTRKDGQLGYLGLSNSAGVELSTAPTSTTSVSIKNNTDLREGFIL
                     LKNLLPGTEFYIGRQVLGFGDNRYVGGRNDGQTGNSFDAFRIKYNYKNFQTEAFTSII
                     AEDSNAGSGNNTANGTKRGSVNDTYLSGLYNTLKFDDFLIDLYIFNIDKKWEQSASPT
                     TSVDRTRQRDDLNTAGLRLSNRTNNNILPKTKNWDWTLESSWQFGKNGQRVGAGWDQL
                     KLTAPNGARIYNENVEYDAKFFIAQTGYTFFDRFRVGIQYSQASGDPNRSDAKVATYD
                     ASFATRSGGFPYFDSGNGIVNAAFWSNTKTKSVHLMWNTETTGRWILVGYDVQKAAVN
                     DAWYSSGGAPNTGLTTENSTGARWGGYKLGEKPGKGIFKEFDLIWQYYMKDYVSIWAG
                     ASVLLAGDSVKNARVNPWAANANDRYTLDGRAYTFFMFVQFAM"
     gene            1923..>2549
                     /locus_tag="PK683_07305"
     CDS             1923..>2549
                     /locus_tag="PK683_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH08293.1"
                     /translation="MKKHTLQFTLAVLTLGLTSIFAKDITLLNVSYDPTREFYVEYNA
                     LFAKYWKSKTGDDVRINQSHGGGGKQARSVIEGLEADVVTLAVSYDLDIVASKGLMAP
                     AWQKSLPDNSTPYTSTIVFVVRKGNPKKIKDWDDLIKDKVSVITPNPKTSGGARWNYL
                     AAWGFAARKFKNDESKIRDFIKTLYKNVPVLDSGARGSSTTFTVNGIGD"
BASE COUNT          832 a          498 c          608 g          611 t
ORIGIN      
        1 accctatttt tacatggttc ccaacgacaa caaggaggga atcagatgaa aggagttctg
       61 atcagaaatt tattaggagt atttattata tgcttctgct gcgagattct ctcacaggaa
      121 acatctaagg aaaagacaaa ggcaaaagag gcagagacgg aacagccgtt aagcctggac
      181 ccgccaaagg acattcaata tgatgctcag ccgaatgacg ggaagggaat accggaaatt
      241 caggcagaac tcgcaaaaga agaaccttat aaaagtcctt ataaaggaaa acttccggga
      301 gaatttatga ggacaatgct tcttgcgccg gatcatcagg atgcagtcag aaaagtggac
      361 agactgtggt ttgcagatat cattcggaca ggatttcagc ttcgcccccg atttgactat
      421 actcataatc tggacttcga caagagaaca caggatgaca ggaattttgg aacacagaat
      481 tctcagctct ggtttgtgat tgatcctcat ccgaatgtgg ctgcaaagat tacaattcag
      541 gatgtgcgtg tattcggagg agcacagaca agaaaggatg gtcagctcgg ttatctcgga
      601 ctttcgaatt cggctggggt ggaactttcc actgcaccga catcaacaac atctgtcagc
      661 attaagaaca atactgatct gagagaagga ttcattcttc tcaaaaatct tcttcctgga
      721 acagagttct atataggaag acaagtactg ggatttggag acaacaggta tgtgggaggc
      781 cgaaatgacg gtcagactgg aaactcgttt gatgcattca gaataaagta taattataaa
      841 aactttcaga ctgaagcatt cacttccatt atcgcggaag acagcaatgc aggaagcgga
      901 aacaatactg caaacggaac caaaagaggg tcagtaaatg atacttacct gtcagggctg
      961 tacaatacac tgaagtttga cgactttctc attgatctgt atattttcaa tattgacaag
     1021 aaatgggaac aaagtgcttc accgactaca agtgtagaca gaacaagaca gagagatgat
     1081 ctgaacactg caggtctgcg gctctccaac agaacaaaca acaatattct gccaaagaca
     1141 aagaactggg actggacact ggaatcttcc tggcagttcg gaaagaacgg tcagagagtc
     1201 ggtgcgggct gggatcagct gaaactcaca gctccgaacg gtgcaagaat ttataatgaa
     1261 aatgttgagt acgatgcaaa gtttttcatt gcacagacag gttatacatt ctttgacaga
     1321 ttcagagtgg gtattcagta ttctcaggct tcaggagatc cgaacagatc agatgcaaaa
     1381 gttgcaacat atgatgcttc atttgccaca aggtcgggag gttttcctta ctttgattct
     1441 ggaaacggta ttgtgaatgc tgctttctgg tcaaacacaa agaccaaatc agtccacctg
     1501 atgtggaata cagaaacgac cggaagatgg attcttgtag gttacgatgt tcagaaggct
     1561 gcagtcaacg atgcatggta ttcttccgga ggagcaccga acacaggact gacaacagaa
     1621 aattcgacag gagcaagatg gggcggatat aaacttggag aaaaacccgg caaaggaatt
     1681 ttcaaggaat tcgatctgat ctggcagtac tatatgaaag actatgtttc catatgggca
     1741 ggagcatccg ttcttctggc gggagattca gtgaaaaacg caagagtcaa cccttgggca
     1801 gcaaatgcaa atgacagata cactctggac ggaagagcat acacattctt tatgtttgtc
     1861 cagttcgcaa tgtgagtgaa tctcatttta caattcagca atataatcat cggaggaaaa
     1921 agatgaaaaa acatacacta caattcacgt tggccgtttt aacattagga ctgacttcaa
     1981 tatttgcaaa ggacattact cttctgaatg tatcttatga tccgactaga gagttttatg
     2041 tggaatacaa tgccctattt gcaaaatact ggaagtcaaa gacaggagat gatgtcagaa
     2101 tcaatcagtc acacggagga ggcgggaagc aggccagatc agtcattgaa ggactcgaag
     2161 ctgatgttgt tacacttgca gtgtcttatg atctggacat tgtagcctcc aaagggctga
     2221 tggctccggc ctggcagaaa agcctcccgg acaacagcac tccttatact tccacgattg
     2281 tattcgtagt gagaaaggga aatccgaaaa agatcaagga ttgggatgat ctaatcaagg
     2341 acaaagtcag tgtaattact cctaatccga agacaagcgg cggcgcaaga tggaactatc
     2401 tggcagcatg gggttttgcc gcaaggaaat tcaaaaatga tgaatcaaag atcagagact
     2461 tcatcaagac tctttacaag aatgttccag tgctggattc aggtgccaga ggttccagta
     2521 caacattcac agtcaacgga atcggtgat
//
LOCUS       DAOQGJ010000390         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25860_length_2548_cov_2.874850, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000390 DAOQGJ010000000
VERSION     DAOQGJ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25860_length_2548_cov_2.874850"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PK683_07310"
     CDS             complement(<1..970)
                     /locus_tag="PK683_07310"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNH08294.1"
                     /translation="MNGSVFRKELLELEPYTPGEQPPVTGKLIKLNTNENPYPPSPKI
                     EKAVQEIFAKGYLRKYPHPSSAKLRQAAAEVYGITEDMVLVTNGSDEGLALLFRAVLG
                     KNSKLIMPYPTYSLYTVLSEIQMNGVTVVKVPLKEDLHFDFERLGAEKGELLAFAHPN
                     APTGILEKKEEIRNLIRNFPGAVLSDEAYIDFAEEGASFLSEVNSLPNLFVTRTFSKS
                     YSLAGLRVGLIISNPDNIALLMKLKDSYNLGMLEETIAAASLKDRAYFRKTVKTIVSS
                     REKMKKSLENLGFTTADSSANFLFTKPPEANYELVKKFQFSEFEEKV"
     gene            complement(967..2469)
                     /locus_tag="PK683_07315"
     CDS             complement(967..2469)
                     /locus_tag="PK683_07315"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNH08295.1"
                     /translation="MDDFVRIFDTTLRDGEQCPGAAMSEDEKLEIAHHLAKMGVDVIE
                     AGFPVSSPVQFQAVERISREVEGPVIAALARAMKNDIQTAASALRPAKKRRIHTFLAT
                     SPIHMKYKLGKTAAEVLKMAPEAVRMAREFVDDVEFSPEDGTRSDWEFLREVCEAVIE
                     AGVGTINVPDTVGYTTPEKYGEMFNYLIKNVRGADKIIFSAHCHNDLGLAVANSLSAI
                     KNGVRQVECTINGIGERAGNTAMEEVVMALRTRKDFYGITTKVKTELITRASYLVKTI
                     TGMVVQPNKAIVGANAFAHESGIHQDGVIKNRETYEIMTPESVGLESNRMVLGRHSGR
                     AGFKDRIIRLGFSPSAEELETAYSRFLEIADKKKEIFDDDLVALFSSQSRQVIPSKYK
                     LVYYQVTSGSNTVPTATVKIAVDSEIREESCTGDGPVDAVFKAVQKIVQISPILNKLI
                     ISPVTKGTDALAEASVSLTYEDRVVVGKGSSTDIVEASAISFLDAINRLQ"
BASE COUNT          628 a          593 c          531 g          796 t
ORIGIN      
        1 agaccttttc ttcgaattct gaaaactgaa attttttaac tagttcataa ttagcttccg
       61 gaggtttggt gaacagaaaa tttgcactgg aatctgcagt cgtaaatccc agattttcca
      121 gcgatttctt cattttttct ctcgaactta cgattgtctt tacggtcttt ctgaagtagg
      181 ctctgtcctt cagtgaagca gccgcgatcg tttcttcaag cattcccaga ttgtaggagt
      241 ctttcagttt cattaaaaga gctatattat caggattgct gataataaga cccactctaa
      301 gacctgcaag ggaataagac ttggagaatg tccttgtgac aaacagattt ggaagactgt
      361 ttacttcaga aaggaaactt gccccttctt ccgcaaagtc gatgtatgcc tcatcggaca
      421 gaactgctcc aggaaagttt ctgatcagat ttctgatttc ctccttcttt tccagaattc
      481 ctgtcggagc gttcggatgt gcaaatgcca gaagttctcc tttttcagct ccaagtcttt
      541 caaaatcaaa atgcagatcc tccttaagag ggacttttac aacggtcact ccgttcatct
      601 gtatttccga aagaacagta taaagagagt aggtgggata cggcataatg agttttgagt
      661 tttttcccag aactgccctg aaaagaagag caagcccttc atcagatccg ttcgtcacaa
      721 gcaccatatc ttcagttatt ccgtagactt ctgcagcagc ctgccgcagt tttgcagaac
      781 ttgggtgcgg atattttctg agatatcctt ttgcaaatat ctcctgaaca gctttttcta
      841 ttttaggact cggcggataa ggattctcat ttgtgttgag tttgatgagt tttcctgtga
      901 caggaggctg ttctcccgga gtgtagggct caagctcgag aagttctttt ctgaatactg
      961 aaccgttcat tggagcctgt tgattgcatc cagaaatgaa atggcagatg cttccacaat
     1021 atcagtagag cttccttttc ccactacaac cctgtcttca taagtcagac tcacagatgc
     1081 ttccgcaaga gcatccgttc ctttggtaac aggggagata atcagtttat tgagtatcgg
     1141 actgatctga actattttct gaactgcctt gaagactgca tccaccggtc cgtctcctgt
     1201 gcaggactct tctctgattt ctgaatccac cgcaatcttg acagtggcag taggaactgt
     1261 attggaacct gaagtgacct gataatatac gagtttatat ttgctgggaa tcacctgtct
     1321 ggactgactt gagaaaagag ctaccagatc atcatcaaag atttcctttt tcttgtctgc
     1381 aatttcgaga aatctgctgt aggcagtctc cagttcctct gcgctcggag agaaaccgag
     1441 acggatgatt ctgtccttga accctgcacg tcctgaatga cgtcccagaa ccatcctgtt
     1501 cgattccagc cctacggatt caggagtcat gatttcatac gtctctctgt ttttgattac
     1561 accgtcctgg tgaattcctg attcatgagc aaatgcattt gcacctacta ttgccttgtt
     1621 cggctgaact accattcctg taatcgtctt cacgagatag gatgctctgg tgatcagttc
     1681 agtctttact ttggttgtaa ttccgtaaaa atcctttctg gtgcggagtg ccattacgac
     1741 ttcttccatt gctgtgtttc ctgctctttc tcctataccg ttgattgtgc attccacctg
     1801 acgcacaccg tttttgattg cagagagaga attggcaacc gccagtccaa gatcattgtg
     1861 gcagtgagcg gaaaatatga ttttgtctgc tccccttaca tttttgatca ggtagttgaa
     1921 catctcaccg tatttttcag gagtagtgta gccgactgta tcaggtacat tgattgtgcc
     1981 tacgcctgcc tcaatgacag cctcgcagac ctctcttaaa aattcccaat ctgatctggt
     2041 tccgtcttcc ggagaaaatt ccacatcatc cacaaactct cttgccatac gcacggcctc
     2101 tggagccatt tttagaacct ctgcagcagt ttttcccagc ttgtatttca tatggatagg
     2161 ggaggtggca aggaatgtat gaattcttct tttttttgca ggacggagtg cggaagcggc
     2221 agtctgaatg tcatttttca ttgcacgtgc taaggctgca atgacagggc cttccacctc
     2281 tctggatatt ctttccactg cctgaaactg aaccggggaa gacacaggaa aacctgcctc
     2341 aatgacatcc actcccattt ttgccagatg gtgagcgatt tccagttttt catcctcgct
     2401 cattgcagca ccaggacatt gttctccatc cctgagagtt gtatcaaaaa ttcttacgaa
     2461 atcatccatg ttttcctccg gaaatgcatg atgattgaaa gggttttaga tcaagttatg
     2521 cagtcaaccg gaaaaaacgt ctgatttt
//
LOCUS       DAOQGJ010000391         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_25922_length_2545_cov_3.062249, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000391 DAOQGJ010000000
VERSION     DAOQGJ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25922_length_2545_cov_3.062249"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..>2545
                     /locus_tag="PK683_07320"
     CDS             43..>2545
                     /locus_tag="PK683_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08296.1"
                     /translation="MLSDAFHLSPGEAEPLAELLCSKTEGNPFFLRETLIKLHKEKLI
                     IFDADRGKWACNIADIRNIQFAENVIDLLVRKIAGFPEETVSILRLASCIGNRFDVRT
                     LGIISGLDNKQVLKLLLPAVQEDLIFSSQNPQQIYFQFQHDKVQQACFEQMNPSECAY
                     IRLKIGKLLLNSVSSEDMYERIFEITEHLNAGSYLIGDENEKISLIEMNLAAAEKAKQ
                     SAAYSSSLLYLRKAGELFYPGLWERKFSLSYRLYKELAEAEHLNGNFENSVELVEYAE
                     QNCANRTDKGNLLNLLITIYSVQGKFDKAYQIIIKILSMFSIQIEGKNSGEIFQEEVR
                     EIRSVIGDRSFSSLDSLPLIKDPEQELIARTLANATAASYQYATHLFPLLGAKLVNLY
                     LKYGNMQDSYGYALFGIMLNVYFADYKQAYECFELTYRLSEKYNNVSAKAKAANLLAN
                     YGSPFLKPLKDSGRINRECIEAGLESGEFEHGGYGIGNDLVNSFFQGMYLGEIRLKIS
                     ELKSLAEKFKHFIALDIISGVELVLSFLNGNRENDEYKTEEKFLSSFQSDKGKYPLAM
                     YGIMKEYSNFIMDRPERCIREASEKDELLQYLTGTYLAVEHAFIQTVSLTSLYETFSA
                     EEKGKASAKISENADLLRNWSETCPENFNHQYLLVLAETSRIKGDFWNALNYYDSAAS
                     SANKNGFFQNEALANELAGKMFLLQNKTEFAYTYLIKSYHIYETWGAVNKCEALRRAY
                     PNVFQQKENPQSPYININDLSSFNRTLRYISSRLNEKLDLQTVIRTSHILSEEIDFDR
                     LIVKIMQSLIENSGADRGAFITVEKSRKDGIEFLFL"
BASE COUNT          785 a          477 c          542 g          741 t
ORIGIN      
        1 aaaatagatc ttcttcctct tgcaccggag aatataagtc aaatgctgtc tgatgctttt
       61 catctttctc ccggggaagc tgaacctctc gcagaacttc tgtgcagtaa aactgaaggt
      121 aatccttttt tcctccgtga gactttaatt aagctgcata aggaaaaatt aattatcttt
      181 gatgcagacc gggggaaatg ggcatgcaat attgcagata ttcggaatat tcagtttgca
      241 gaaaatgtaa tcgatcttct tgtcagaaag attgccggtt ttcctgaaga aacagtcagc
      301 atcctcagac ttgcttcctg tatcggaaac agatttgatg taagaacatt gggtattatc
      361 agcggtttag acaataaaca ggttttaaag cttcttctgc ccgcagttca ggaggatttg
      421 atattctcct ctcagaatcc tcagcagatt tattttcaat ttcagcatga taaggtccag
      481 caggcctgct tcgaacagat gaatccaagt gagtgtgcat acattcgtct caaaatcgga
      541 aagcttctgc tgaactcggt ttcttcagag gatatgtacg aacgaatatt tgaaattacg
      601 gaacatctta atgcagggag ttatttaatc ggtgatgaga atgagaaaat cagtctgatt
      661 gaaatgaatc ttgcggccgc tgaaaaggca aagcagtctg cagcatattc ctcatctctg
      721 ctttatctta ggaaggcagg agaacttttt tatccaggac tttgggaaag aaaattttct
      781 ctttcataca gactgtacaa ggagctggca gaggcagaac atctgaacgg caatttcgaa
      841 aattctgtag aattggtaga gtatgcagag cagaactgcg caaacagaac ggataaagga
      901 aaccttctga acctgctcat tacaatctat tctgttcagg gaaagtttga caaagcatat
      961 cagattatta ttaaaatact ttctatgttc agtattcaga tagaaggaaa aaattccggt
     1021 gaaatatttc aggaagaagt cagagagatc agatcagtca taggggacag gagtttttct
     1081 tctcttgatt ctctgccttt gatcaaagat cctgaacagg aacttattgc cagaactctt
     1141 gcaaatgcca ctgcggcatc ctatcagtat gcaactcatc tctttcctct cctcggtgcc
     1201 aagcttgtaa atctgtatct gaaatacggg aatatgcagg attcttacgg atatgcgctt
     1261 ttcggaatta tgctgaatgt ctattttgca gattataagc aagcttatga atgttttgaa
     1321 cttacttaca gactgagtga aaagtataat aatgtttccg caaaggcaaa ggcggcaaat
     1381 cttctggcaa attacggttc cccttttctg aagcccctga aggattcagg acggataaac
     1441 agagaatgca ttgaggcggg actggaatcc ggtgagtttg agcatggagg ttacggaatt
     1501 ggaaatgacc ttgtaaattc cttttttcag ggaatgtatc ttggggagat acgtctcaaa
     1561 atcagcgaac tgaagagtct tgcagagaag ttcaagcatt ttatagcact tgacattatt
     1621 tccggagtgg aacttgttct ttcttttctc aatggaaaca gagagaacga tgaatacaag
     1681 actgaggaaa agttcttaag ctcattccag tctgataaag gcaagtatcc ccttgctatg
     1741 tacggaataa tgaaagaata ttcaaatttc attatggata gacctgaaag atgcattaga
     1801 gaagcttccg aaaaagatga gcttcttcag tatctgaccg gaacctatct tgcagttgaa
     1861 catgcattca ttcagacagt ttcactgacc tcgctttacg aaacattctc tgcggaagaa
     1921 aagggaaaag cttctgcaaa aatttcggag aatgctgatc ttctcaggaa ctggtccgaa
     1981 acctgtcctg aaaattttaa ccatcagtat ctgcttgtcc ttgctgaaac ttcgaggatc
     2041 aaaggcgatt tctggaatgc cttgaattat tatgacagcg ctgcatcgtc tgcaaataaa
     2101 aacggttttt ttcagaatga ggctctggca aatgagcttg ccgggaaaat gtttctgctc
     2161 cagaataaga cagagtttgc gtacacttat ctgattaagt cttatcatat atatgaaact
     2221 tggggtgctg tcaataaatg tgaagccctg cgaagagcat atccgaatgt attccaacag
     2281 aaggaaaatc cccagtcccc atatataaat attaatgact tatcttcctt taacagaacg
     2341 ctcagatata tttcaagcag gctgaatgaa aaactggatc ttcagacagt catcagaaca
     2401 tcccatatcc tttctgaaga gattgatttc gacagactca ttgtaaaaat tatgcagagt
     2461 cttattgaaa actcaggagc agacagaggc gccttcatta ctgttgagaa aagcaggaag
     2521 gacgggattg agtttctttt tcttg
//
LOCUS       DAOQGJ010000392         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26004_length_2541_cov_3.311746, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000392 DAOQGJ010000000
VERSION     DAOQGJ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26004_length_2541_cov_3.311746"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK683_07325"
     CDS             complement(<1..225)
                     /locus_tag="PK683_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNH08297.1"
                     /translation="MLFNSIPFLLLFALTYCIYWISAQKWRKYILLLSSIVFYSYNSP
                     YLMLHFGAVIAVNYLFAEQLLKRKLAGQDTG"
     gene            complement(225..1202)
                     /gene="sppA"
                     /locus_tag="PK683_07330"
     CDS             complement(225..1202)
                     /gene="sppA"
                     /locus_tag="PK683_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNH08298.1"
                     /translation="MKKISIFLIPFFFLKCVAFNTIRAFDFAGEKSLELVEQRIESHG
                     TDKILVISVEGEISDSPKSGGGLFSMGSHDSMVAVIKEELKKAKNDSQIKGVILKIDS
                     PGGGVTASDIIYHELMEFKELKKIPVVALFMDVAASGGYYVAMASDGIVSHPTAITGS
                     IGVIISGINVKDGLEKIGIKDQSITSGANKSILSPLREFTPEQRKIIQSVVDQMYERF
                     FKIVKKNRSKVADARLRLIADGRIFTAEQALQEGLVDRVGYLQDAVDHLKKMSNYTGS
                     DSPSIIHYGYDKRQNRNFYHNSYAEPGNDMISSLLKLNSQRKFMYLWKL"
     gene            complement(1250..1927)
                     /locus_tag="PK683_07335"
     CDS             complement(1250..1927)
                     /locus_tag="PK683_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08299.1"
                     /translation="MKIEDISYSDAYLPLQDSRIYYKGHGQTESSSEQNSALHVIAHE
                     MQHVAEFKAEALRDKVEIRNIDIDINFEMRNGKLVAVGGKAKMTAEKEEEHSHQDDSF
                     FHPDLSEVKSEFFSKTGFITYEEKEIAGRLNTIQDNVQDLLTHPFYRDSEKRSIDDYR
                     EEKHLIQREEHISRIDDELKQMKKEKKVQKNDPLMFWINSIQGTISAGTERIGHLPKM
                     INITMRG"
     gene            complement(1975..>2541)
                     /locus_tag="PK683_07340"
     CDS             complement(1975..>2541)
                     /locus_tag="PK683_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH08300.1"
                     /translation="VFDEHYRFLDYHEEIGFSGADAFSNIFATGSVTKCFGVTGLRIG
                     WAAGNADVIPKLRGLKDYITHTVNPVSEFLALRILEKRQSISAALKKRVGRNLEVFRK
                     YFPLLHSLKSANLPEGGLVFFPELKAGLASESYSDWLLENADVFVLPGSSFETEGFIR
                     IGLGETEDRFEKGIKKWAEAEKKLASEK"
BASE COUNT          682 a          589 c          489 g          781 t
ORIGIN      
        1 ccctgtgtcc tgacctgcca gctttctttt cagaagctgt tcagcaaaaa gataattgac
       61 tgcaatcaca gccccaaagt gaagcatcag atagggactg ttgtaggaat aaaaaacaat
      121 tgaagaaaga agaagtatgt attttctcca tttctgcgct gaaatccagt aaatgcagta
      181 agtcagcgca aaaaggagga ggaaaggaat tgaattaaaa agcatcagag cttccagaga
      241 tacataaatt ttcgctggct gttcagtttc agaagactgc tgatcatatc atttccaggc
      301 tctgcatatg aattatgata aaagtttctg ttctgtcttt tgtcataacc gtaatggatg
      361 atgctcggag aatcggaccc agtataattg ctcatttttt tcagatggtc aactgcatcc
      421 tgaagataac ccaccctgtc gacaagtccc tcctggagag cctgctctgc agtaaaaatt
      481 cttccgtccg caatcagtct gagtcttgca tctgccacct tggaacggtt cttttttact
      541 atcttgaaaa atctttcata catctggtcc actacggact ggatgatttt tctctgttca
      601 ggagtaaatt cccgcagagg ggaaaggatg cttttgtttg caccggatgt aatgctctga
      661 tccttgatgc cgattttttc cagaccgtcc ttcacattga ttccgctgat gatgactccg
      721 atagaccctg taatcgccgt aggatgcgaa actatcccgt cgctggccat cgctacatag
      781 tacccgccgc tggcggccac atccataaag agagcaacaa cgggtatttt cttcagttcc
      841 ttgaactcca tcagttcatg gtaaataata tcgctggcag tgaccccgcc gccgggtgaa
      901 tctatcttca gaatgactcc ctttatctga gagtcatttt tggccttttt cagctcttct
      961 ttgatcaccg caaccatgga atcatgagaa cccatactga aaagcccgcc tcccgattta
     1021 ggagaatcag aaatttcacc ttccacggaa atgactaaaa tcttgtcagt tccatgggac
     1081 tcgattctct gttcgacaag ctccagggat ttttcccccg caaaatcgaa ggctctgatt
     1141 gtattgaatg caacacattt gagaaaaaag aaaggaatta gaaaaattga aatttttttc
     1201 atacagtcag aatcaaatga ttctgactgt atgcaagcaa aaagtgaact cagcctctca
     1261 tagtgatatt gatcattttg ggaagatgac cgattctttc ggttcctgcg gatatagttc
     1321 cctgaatgga attaatccag aacatcaaag gatcgttttt ctgaaccttc ttttcctttt
     1381 tcatctgctt cagctcatca tcgattctgg agatatgctc ctctctctgg atcagatgtt
     1441 tttcttccct gtagtcatca atgcttcttt tttcggagtc tctatagaaa ggatgagtca
     1501 gaagatcctg cacattgtcc tgaattgtat tcagacgtcc tgcaatttcc ttttcttcat
     1561 aagtgatgaa ccctgttttg gagaaaaatt ctgatttgac ctcggaaaga tcagggtgaa
     1621 aaaaactgtc atcctgatga ctgtgctctt cttccttttc ggcagtcatc tttgcctttc
     1681 cgcctacagc gaccagtttc ccgtttctca tctcaaaatt gatgtcaatg tcgatattcc
     1741 ggatttccac tttgtcgcgc agggcctcgg ccttgaattc tgccacatgc tgcatttcgt
     1801 gagctatgac atgaagagcc gagttctgct ctgaggaact ttctgtctgt ccatggccct
     1861 tataataaat tctggaatcc tgaagcggca gataagcatc agaataagat atatcttcta
     1921 ttttcatatc attctccgat tttcattttt ttggtatggc ataaaattac agactcattt
     1981 ttctgatgca agtttttttt ctgcttccgc ccattttttt atgccctttt caaatctatc
     2041 ctctgtctcg cccagtccaa ttcggataaa tccttccgtt tcgaaactgc tcccaggaag
     2101 cacaaaaaca tctgcatttt cgagaagcca gtcggaatag gactcgctcg caagccctgc
     2161 cttaagttca ggaaaaaata caagtccccc ttcaggaaga tttgcagact tcagtgaatg
     2221 aagaagagga aaatattttc gaaagacttc cagatttctg ccgacccttt ttttcagtgc
     2281 tgcagaaata ctctgtcttt tttccagaat tcggagagcc agaaattcgg atacagggtt
     2341 cactgtatga gtaatgtaat ctttcaggcc tctcagtttc ggaatgacat ctgcattgcc
     2401 tgcagcccag ccgattctga gtcctgtcac tccgaaacat tttgtgacag atcctgttgc
     2461 aaaaatatta ctgaacgcat ctgcacccga aaaaccgatt tcttcatgat aatccagaaa
     2521 tctgtaatgc tcatcaaaaa c
//
LOCUS       DAOQGJ010000393         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26008_length_2541_cov_2.629525, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000393 DAOQGJ010000000
VERSION     DAOQGJ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26008_length_2541_cov_2.629525"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PK683_07345"
     CDS             complement(<1..181)
                     /locus_tag="PK683_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08301.1"
                     /translation="MKNESVGALLYSVFFWILNNLYYFLLISSPRRNDSGELCKAFGG
                     QSAGLLGASYASRAAL"
     gene            complement(231..1163)
                     /locus_tag="PK683_07350"
     CDS             complement(231..1163)
                     /locus_tag="PK683_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNH08302.1"
                     /translation="MKNLYVRVPGTSANLGSGFDILGLGLDIYNVFKFNFNGKGRFRL
                     YLENGDEFRLSRPKNFIFQAYKRYFHTFLNKEIFPDFEAVLKLDLPMKGGLGSSASAL
                     AAGFAAARYVHSKEFTSVPLPTEERFLYELAMMEGHPDNTTPAVLGNFILSYFTGKSL
                     VYFKEKFPDNISLYLFIPDLQTSTNDSRKKLPESYPVSDVICNMSRVATWMKFIQTKE
                     ISLLPLALDDKIHTSYRLSSQPVLKKVTASAVENSGFWSLSGSGPTLLIYCDKKNSDH
                     FFDAMKNSLSIIKGVNHKFFPTKVSSEGMTVKEI"
     gene            complement(1160..1702)
                     /gene="pth"
                     /locus_tag="PK683_07355"
     CDS             complement(1160..1702)
                     /gene="pth"
                     /locus_tag="PK683_07355"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017850237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNH08303.1"
                     /translation="MSENSKLLITGLGNPGKKYEMNRHNIGFIIADVLAESWNISFDR
                     DSKCQSAKKSGDHLIIIIKPQEYMNLSGKAVQSFMSQYKIHPSNHLVLHDEIDFPFAK
                     IKMKVGGGHAGHNGLRDIMEKTGNGDFHRLRFGVGKPQQKEFVADYVLSNFFDEEKKQ
                     LSELIKDSIKLIEEWVKSRK"
     gene            complement(1699..>2541)
                     /locus_tag="PK683_07360"
     CDS             complement(1699..>2541)
                     /locus_tag="PK683_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNH08304.1"
                     /translation="GERAGNSPMEAVITALHDKLGIRTNIVETEITGISKLVEAFSGK
                     RISDNRPIVGQDVFTQTAGVHADGDKKANLYANPILPERFGRKRTYALGKLAGKASIS
                     ENLKQLGLVLNEDLERKLLEKVIEMGDSGLNVTLEDLPFLIADISGTSITNDFSIETC
                     IVTSGKGVSPSANISVKYKGDLYKEVGLGDGGYDAFMAALTKIADRIGLKLPVLTDYQ
                     VRIPPGGKTSALVETIITWERESDKKDDSFRTVGLDSDQLTAAVMATEKMVNLLLFSE
                     GNQS"
BASE COUNT          723 a          576 c          469 g          773 t
ORIGIN      
        1 atagagccgc ccggctcgcg tagctcgccc ctagcaggcc tgcgctttgc ccgccgaagg
       61 ctttgcaaag ctcgccggaa tcattccttc tgggtgatga aatcaggagg aaataataga
      121 gattgtttaa tatccaaaaa aatacagaat acaaaagtgc accgacggat tcatttttca
      181 atcaggttat ctggattgag ctttacaaaa aggagcttaa tatacagctt ttaaatttcc
      241 ttaactgtca taccctcaga cgaaaccttt gtagggaaaa atttatgatt tactccctta
      301 ataattgata atgaattttt catggcgtca aaaaaatgat cagaattctt cttatcacag
      361 taaatcagca gagtagggcc gcttcccgat aaagaccaga atcctgaatt ttccactgcg
      421 gatgccgtca ctttcttcag aacaggctga cttgaaagcc gatatgaagt atgaatttta
      481 tcatccagag ccaatggaag aagtgatatt tcttttgtct gaataaattt catccaagtc
      541 gctaccctgc tcatattgca aattacatcc gagacgggat atgattccgg aagttttttc
      601 cttgagtcat tcgtagaagt ctgcaggtcc gggataaaaa gataaagact gatattatca
      661 ggaaattttt ccttaaaata taccaggctt tttccagtaa aataagacag tatgaaattg
      721 cccaatacgg ccggtgtagt gttgtccgga tgaccttcca tcattgcaag ttcatagagg
      781 aatctttctt ctgtaggcag aggaacagaa gtaaattctt ttgaatgtac ataccgggcg
      841 gcggcaaatc ctgctgcaag agcactcgca ctcgaaccca atccgccttt cataggaagg
      901 tcaagcttta acaccgcctc aaaatcagga aaaatttcct tgttcaggaa agtatgaaaa
      961 tatcttttat aggcctgaaa gataaaattt ttgggtctgc tcagtctgaa ttcatcaccg
     1021 ttctcaagat ataaccgaaa tctgcccttt ccgttgaaat taaatttaaa tacattatat
     1081 atatccagac caaggccaag aatgtcaaat cctgatccca gatttgcaga agtgcctggt
     1141 actcttacat aaagattttt cattttctgc ttttcaccca ctcttctatc agcttaatag
     1201 agtcttttat cagttcagaa agctgctttt tttcttcatc aaaaaaattt gaaagaacat
     1261 aatcagcaac aaattccttc tgctgcggtt tccctacgcc gaaacgaagt ctgtgaaagt
     1321 ctccgtttcc agttttttcc ataatgtccc tgagaccatt atgaccggca tgaccgcctc
     1381 cgaccttcat tttgatcttt gcaaaaggaa aatcaatttc atcgtgaaga accagatgat
     1441 ttgacggatg aattttatac tggctcataa aactctgcac tgccttgccg gaaagattca
     1501 tatactcctg gggctttata atgataatta aatgatcacc tgatttcttt gcagactggc
     1561 actttgagtc tctgtcaaat gagatattcc agctctcagc cagaacatcc gcaataatga
     1621 aaccgatatt gtgccgattc atttcatatt tttttccagg atttccaagt cctgtaatta
     1681 aaagtttaga attttcactc aagactgatt tccctctgaa aacaaaagaa gatttaccat
     1741 tttttccgta gccattactg cagcagtcag ctgatcagaa tccagcccga ctgttctgaa
     1801 gctgtcatcc tttttgtcag actctctctc ccaggtaatg atcgtttcca ccaaggctga
     1861 tgttttgcct cccggcggaa ttcttacctg atagtcagtc agaaccggta gttttaagcc
     1921 gatacgatct gcaatcttag tcagagcggc cataaaagcg tcgtaccctc cgtcacccag
     1981 accgacttct ttataaagat cgcctttata ctttactgaa atatttgcgg aaggagaaac
     2041 gccttttcca gaagtgacaa tgcaggtttc tatcgaaaaa tcatttgtga tgcttgttcc
     2101 agagatatct gcaatcagaa aaggaagatc ttccaaagtg acattcagac cgctgtcacc
     2161 catttcaatt accttttcca gaagcttgcg ctcaagatcc tcattcagaa ccagaccaag
     2221 ctgtttcaga ttttctgaga tgcttgcctt gcccgcgagc tttccaagtg catatgttct
     2281 ttttcgtccg aaacgttcag gcagaatcgg gtttgcatac agattcgctt ttttatctcc
     2341 gtcggcatgc acacctgccg tctgagtgaa aacatcctga cctacaatcg gtctgttgtc
     2401 tgaaattctt tttccgctga atgcttccac cagtttactg attccggtaa tttcagtttc
     2461 aacgatattt gttctgattc ccagtttatc atgaagagca gtgatgactg cctccatcgg
     2521 agagttgcct gctctttctc c
//
LOCUS       DAOQGJ010000394         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26029_length_2540_cov_2.265594, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000394 DAOQGJ010000000
VERSION     DAOQGJ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26029_length_2540_cov_2.265594"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PK683_07365"
     CDS             <1..1584
                     /locus_tag="PK683_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08305.1"
                     /translation="FNADIRRKLKRSFWNIFRFSDKPVERDVQIPEIIEWDGISAAGR
                     TVSDGKYTYRLKLFFEDNTEWKSDDAVIYSDSKSPYCRLETADRLITPNGDKFLDQLS
                     VRQDFLGDPADQWAGQIRNSKGETVRNFRWDTSRLPKSFVWDGRDDSGRISDNGFYEY
                     ELTGRDVSENAFSCFLKGILISSQQTQADIFSEESVFSPNFDGFADTVKFSMSLGFDS
                     RRLNKSKLVIYNGEPMPGKEIFSHSLSSSERFFIWDGRKNDGGFVSGDMLSYRLEYEM
                     DGNAYVSETRKLEAVGRKVRASLKVSEDEFHPDNDNELDYIYLYPNLRDADVKTWKLS
                     VVERHGHGNVPRKRVVRSWKGIGKLPEKIFWDGTLNDGSTVGSLAVLDIYLSITGPNS
                     DTKLYLVYNYETGISVYQKGERLKMSFPEYLVEKDEKRIISGIKSLFPRYPGYLIEVQ
                     SHSRQEGDNRKNMKKTELRSLDFYKKMFGTDRVQGKYNYRGFGEVEPMFLEEDDYKQE
                     KNERIDIQFSLPKDGSEKK"
     gene            1581..2540
                     /locus_tag="PK683_07370"
     CDS             1581..2540
                     /locus_tag="PK683_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNH08306.1"
                     /translation="MNSTRERLEKKLALIQIISSNPQIGLDDLQEIAKFESKLELKKN
                     LGELFMVGMHPYTPADYIEVEYDGNGLDIRLPVNLEKTIGLNIQEWVILRKLLDNDDI
                     WSRLKADKTVLNSLKKKIAEVVSYSEFLEFESVRERIYKAIAERKKIRFSYKGHHDSV
                     PENRETEPWFIFGEKTQYLVGYSSEKKGMRNFRLDSMNDLEILNEPISFEPDEMDKEN
                     HMNSFFSFINEKQESSEWAEIAVLPSAHYNLSKELSLGPVQGKIALNGKEYFKFNAKI
                     IEKSWFLNIVKGYGESVFVLSPDSLTSVLANSLDTVQIPELGA"
BASE COUNT          808 a          437 c          620 g          675 t
ORIGIN      
        1 ttcaatgcag atatcagaag aaaactgaaa agatccttct ggaatatttt ccgtttttcg
       61 gataagcctg tggaacggga cgtgcagatt ccggaaatta ttgaatggga cgggatcagt
      121 gcggcaggca gaactgtctc agacggtaag tatacttatc gtctgaaact gttttttgaa
      181 gacaatactg aatggaaatc ggacgatgct gtcatctatt ccgattctaa gtctccttac
      241 tgcagactgg aaactgcaga caggctgatt acgccaaacg gagacaagtt tctggatcag
      301 ctcagcgtcc gtcaggattt tttaggagac ccagctgatc agtgggcagg ccagatcaga
      361 aattcaaagg gcgagaccgt gcggaatttc agatgggata catcaagact tcctaaatca
      421 tttgtctggg acggaagaga tgattccggc aggatctcag ataacggatt ctatgaatat
      481 gagcttactg ggagagatgt ttccgaaaat gcattctcct gttttttgaa aggaatactg
      541 atcagcagtc agcagaccca ggcagatatt ttcagtgagg agtctgtttt ttctcccaac
      601 tttgacggat ttgcggatac agtgaaattt tcaatgagtc tagggtttga ttccagaaga
      661 ctgaataaat ccaagctggt gatttacaat ggagagccta tgcctggaaa agaaatcttt
      721 tctcattccc tcagttcgtc tgagagattt tttatctggg acggaagaaa gaatgacgga
      781 ggttttgtgt cgggagatat gctttcctac aggctcgaat atgaaatgga cgggaatgca
      841 tatgtttctg aaacaagaaa acttgaggcc gtaggaagaa aagtcagagc gtcactcaaa
      901 gtctccgaag atgaatttca tcctgataat gataatgagt tggattatat ttatttatat
      961 cccaatctca gagatgcgga tgtgaaaacc tggaagctgt cagttgtaga aagacatggg
     1021 cacggaaatg ttcccaggaa aagagttgtc cgttcatgga aagggatcgg aaaacttcct
     1081 gaaaaaatat tctgggacgg gactctgaat gacggatcga cggtcggctc acttgcagtc
     1141 ctggacatat atctttccat cacaggaccg aactctgaca cgaaactgta tttagtttat
     1201 aattatgaaa cgggaatttc cgtatatcag aagggagagc ggctgaagat gtcttttcct
     1261 gagtatcttg tagaaaagga tgaaaaaaga atcatttccg ggatcaaaag tctcttcccc
     1321 agatatccag gctatcttat agaagttcag tctcactcaa ggcaggaagg cgacaacaga
     1381 aaaaatatga aaaagaccga actgagatcc cttgattttt ataaaaagat gttcggaacg
     1441 gacagagtgc aggggaaata caattatcgc ggatttggag aagtggaacc tatgtttctt
     1501 gaggaagatg attataagca ggaaaagaac gaaagaattg atattcagtt cagtctgccg
     1561 aaggacgggt cagagaaaaa atgaacagta cgagagagcg actggagaaa aagctggctc
     1621 ttattcagat catttcttca aatccgcaga ttggtctgga tgatctgcag gagattgcca
     1681 agtttgaatc gaaactggag ctgaaaaaga atttaggcga actgttcatg gtaggtatgc
     1741 atccttatac acctgctgat tatatagaag ttgagtatga tggaaacggt ctagatatcc
     1801 gtcttcccgt taacctggaa aaaactatcg gtctgaatat tcaggaatgg gtgattctga
     1861 ggaaacttct ggataatgat gatatatgga gcagactgaa agcggacaag actgttctga
     1921 attcactgaa gaaaaaaatt gcagaagttg tttcctattc cgagtttctg gaatttgaat
     1981 cagtacgtga aagaatttat aaggcaatcg ccgaaaggaa aaaaatccgg ttcagttata
     2041 aaggacatca cgactctgtt cctgaaaaca gagaaactga accctggttc atattcggtg
     2101 aaaagactca gtatcttgta ggatacagca gtgaaaagaa agggatgaga aatttccggc
     2161 tggattcaat gaatgatctg gaaatactga atgagccgat ttcatttgaa ccggatgaaa
     2221 tggacaaaga gaatcatatg aactcatttt tcagttttat aaatgaaaaa caggagagtt
     2281 cggagtgggc agaaatagca gtgcttcctt ctgcacatta taatctttcg aaagagctga
     2341 gtctcggtcc cgttcagggc aagattgctc tgaacgggaa agaatatttt aaatttaatg
     2401 caaaaataat tgaaaaatca tggtttctga atatcgtcaa gggatacgga gaatcggtct
     2461 ttgtgctgtc tcccgattca ctgacgtctg ttctggcaaa ctcgcttgat acagtgcaga
     2521 ttcctgaact gggcgcgtaa
//
LOCUS       DAOQGJ010000395         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26054_length_2538_cov_4.174386, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000395 DAOQGJ010000000
VERSION     DAOQGJ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26054_length_2538_cov_4.174386"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..164
                     /locus_tag="PK683_07375"
     tRNA            90..164
                     /locus_tag="PK683_07375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:123..125,aa:Gly,seq:gcc)
     gene            419..1537
                     /locus_tag="PK683_07380"
     CDS             419..1537
                     /locus_tag="PK683_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH08307.1"
                     /translation="MKYIRFNILISICTLLILSFCGEESGRNPVKETQILKHLLGSSH
                     SVQNDSGRPETSESVKSKSSENIPAYDKKEDSPSIDCTDLKCSFPFRKDYREFLQMKY
                     DHQREIHYDSDADGYYYLRFNPSAKSSNRFDLKTRVTFKTLRAAGIRYSLLIDEENQS
                     SSVVARKEKGKTVWTHLLSSVPTLSPMRSGNQIFICTQDGKITSLNMHTGIQNWFFMT
                     PGAVLIRPAAYNHFIIIFDDSGNIFWLDKNTGSLRHKYFLEEMPVISPVLKEGILVLS
                     TPRGYIHGIKAMEAFPAWRDHSLMGRIEALYDSDDGFVLEGSSIVLFNFQTGAKQKFL
                     NEEELRILAKKTPEFLRSPRMLRYAVEREKFNFSVSGL"
     gene            1837..>2538
                     /locus_tag="PK683_07385"
     CDS             1837..>2538
                     /locus_tag="PK683_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08308.1"
                     /translation="MKIILLKNLILFYLEKLKREPYNMPAQKKKTVLKKKTISKKKTA
                     AKKKTVSKKKKAAEKKEEFPPREYYNLISEAYSKLDSPKRKEKLEAVQMMTMAVNMAP
                     EETHGLFQRGQAFERLERYTEALADYQKCISIDPKGVSYYEMSATVMEKLGLLEDAIS
                     FMQKARKLIQYPYQLEYIRRFRDRLKKMQEDCSSVNEKISKDPENAIYYHDRGQILND
                     MGDYWSAIRDFEKALE"
BASE COUNT          808 a          506 c          542 g          682 t
ORIGIN      
        1 tctcaccttc aaaaggatag tttaatggtc gaacatcgtg attatacggg agccacggct
       61 tttctgaatg cagacagatt ggaacccttg ctggaagtag ctcagaggta gagcactggc
      121 ttgccaagcc atacgcgcag gttcgagtcc tgccgtccgc gccaccggtc acacccttgt
      181 tgaaaggggt gtgacccctt tttttgtgaa attctgatcc tgaaagaaga gcatagggat
      241 tatccctaat acccattcct ttgcagtaat ctcctattgc attaaaatac aaagtatgag
      301 ataaataata actgtgtttg cagccgaagc agtgtgcttg tgcaacggcg gtgaatacac
      361 ttttacacag tctcattcaa atgtgcaaaa gtgaaaaatg acaggacttc ttttcattat
      421 gaaatatatc agattcaaca tactcatttc tatctgcacc cttctcattc tctctttctg
      481 cggggaagaa agcgggagaa atccagtcaa ggaaactcag attctgaagc atctgctcgg
      541 ttcttcccat tcagtacaga atgattcggg aagaccggaa acttctgagt cggtaaaaag
      601 taaatcttct gaaaatatac ctgcttatga taaaaaggag gattcacctt ctattgactg
      661 caccgatttg aaatgcagtt ttccgttccg gaaggattac agagaatttc tgcagatgaa
      721 atatgatcat caaagagaga ttcattatga ttcggatgca gacgggtact attatctcag
      781 attcaatccc tctgcaaaat cttctaaccg tttcgatctg aagacacggg tgacattcaa
      841 gacactgaga gcagccggca tcagatattc ccttctgata gacgaagaaa atcagtcatc
      901 ctctgttgtc gcaagaaagg aaaaggggaa aacagtctgg acacatcttc tgagttctgt
      961 tccgacactc tcaccgatga gaagcgggaa tcagattttc atatgcacac aggacggtaa
     1021 aataacaagc ctgaatatgc ataccggaat ccagaattgg ttttttatga ctccaggtgc
     1081 agttctgatc cgccctgcag catacaatca tttcatcatt atttttgatg acagtggaaa
     1141 tattttctgg ctggataaaa atacaggttc tctcaggcat aaatatttcc tggaagagat
     1201 gccggtcatt tctcctgtgc tgaaggaagg aatccttgtt ctctccacac cgagaggata
     1261 tattcacggg ataaaggcta tggaagcttt ccctgcctgg agggatcatt ctctgatggg
     1321 aagaatagaa gcactgtatg attctgatga cggttttgtt ctggaaggaa gcagcattgt
     1381 tctgttcaat tttcagactg gagcgaaaca gaaatttctg aatgaagagg aacttaggat
     1441 tcttgcaaaa aagactcctg aatttctgag aagccccagg atgctccgct atgcagtcga
     1501 acgtgagaaa tttaattttt cggtttccgg attataaata tacgggattg cgtaaaagca
     1561 aaaagcgatt tacacagaga tgcttatgct tcgagaaact cagcacaatt tcttgcagcg
     1621 gcttccacgc ttgcagcagc ttccatactt gcgattattg tctctgcatt ttccgtgcgg
     1681 gactgtgtaa aagcatcgtt ttcttactga gcacacaaag aacaccaaga aaaaaattaa
     1741 taaaatcaag actctcagtg acctctgtgt cctttgcgag aaatattttc tttgttttta
     1801 cacaacccca ggattataaa aactgctcct ccttctgtga aaattatctt gcttaaaaat
     1861 ctgattctgt tctatttgga aaaattaaaa agggagcctt acaatatgcc tgcccaaaaa
     1921 aagaagactg tccttaagaa aaaaaccatc tccaaaaaga agactgctgc aaaaaagaaa
     1981 acagtatcca aaaagaaaaa agctgctgaa aagaaagaag agtttccgcc tagggaatat
     2041 tacaatctga tcagcgaggc ctattcaaaa ctggattctc cgaagagaaa ggaaaaactc
     2101 gaagcagttc agatgatgac tatggcagtg aatatggctc ctgaagaaac tcacggcctg
     2161 tttcagagag gtcaggcttt cgaaagactg gaaagatata cagaagctct tgctgattat
     2221 cagaaatgca tttcaattga tccgaagggg gtatcctatt atgaaatgtc tgcgaccgta
     2281 atggaaaaac tgggacttct tgaagatgcc ataagcttta tgcagaaggc tagaaaactg
     2341 atccaatatc cttaccagct ggaatatatc agaagattca gagacagact gaaaaaaatg
     2401 caggaagact gctcttccgt gaatgaaaag atttcgaaag atccggagaa tgcaatatat
     2461 tatcatgata gaggacagat acttaatgat atgggagatt actggtctgc catccgggat
     2521 ttcgagaaag cacttgag
//
LOCUS       DAOQGJ010000396         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26083_length_2536_cov_4.041112, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000396 DAOQGJ010000000
VERSION     DAOQGJ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26083_length_2536_cov_4.041112"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PK683_07390"
     CDS             complement(<1..758)
                     /locus_tag="PK683_07390"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HNH08309.1"
                     /translation="MQSFFIYLKTRFSAAILFTGLVYFLLSKSGLILAIPGTNASPIF
                     PAAGFALSAVLLLGKRSLFGIFLGSLFGNIFTLSDDLSFQQNILASILTGIGASLQAY
                     SGYFFLKKMAKETEPFFYKNDVLIFSVFSVFISSVISASFGLTGLYLSGLIGKDSVPA
                     VWLTWYLGDAMGVLIAAPLFLVWRIPALSRFSALKYAEISAGSVVAFILGIFLLQSTY
                     PLYFIFIIILLAASLRYMQFGAVLFSGIISAVLI"
     gene            complement(837..>2536)
                     /locus_tag="PK683_07395"
     CDS             complement(837..>2536)
                     /locus_tag="PK683_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08310.1"
                     /translation="FHIHRMNVFMDNIIYDRNPKLFTSYELLKNFQQTARSVRNLVIM
                     TNPEDLLKEKNTVIQLMNTEIPKQLNYLNTHSNTAKGKELTGILIEKNNLMSVQIDLI
                     LKAVENNKKEEAIALINSKAKNSMTQIYFAMTIAEYADFQKSILEENTGIIHSTIENT
                     KAASVIIFFISFIISIVIVAYLIKYITDSLQKLTESIEKISSGDLKVKINTEKNDEIG
                     KIGKSFERMVSTINGLIIDLNSISDSATAGKLDLRADTSVYQGDYKKLLDGFNRTLDA
                     VILPIQEVISISEEVSRGNLSTRVKGTYNGEFNLLQVSFNTTIGIMEKMILELIHIEN
                     DLAGSTDELWSVTDKLSSSASQQAANAEESAAAIETITSAVIHNNKNTKYAESIAISA
                     FNKAATVERAMSDTLTAMKTIAAKTKIIEKITAQTNLLALNASIESVRAGDHGLGFSV
                     VATEVKKLADESIIAAKEIQELARNSVMTAENAERLVKEILPDIKETAELVNEISTAS
                     EEQKNGISQINTAMIQIAQTSQENSEVSEELSATADLLKKNSEKLRKNISFFKILEK"
BASE COUNT          724 a          488 c          487 g          837 t
ORIGIN      
        1 atgatcagaa ctgccgatat gattcctgaa aacagaacag cgccgaactg catatacctc
       61 agagatgctg cgagaagaat aataatgaaa ataaaataca aaggatatgt gctctgaaga
      121 agaaaaattc ccagaatgaa tgcaaccact gagccggcgg aaatctctgc atatttcaaa
      181 gcactgaatc tggaaagtgc aggaatcctc cacactagaa aaagaggagc agcgatcagc
      241 acacccatcg catctccaag ataccatgta agccagactg caggcacaga atctttccca
      301 atcagtcccg acagatacag tcctgtcagt ccgaaggatg cactgatgac ggatgaaatg
      361 aaaacagaga atacggagaa tatcagaaca tcatttttat aaaagaaagg ctcagtttct
      421 tttgccattt ttttcaggaa aaaatatcct gaatatgcct gaagggatgc cccgatgcca
      481 gtcaggatgg aagccagaat attctgctga aagctcagat catctgataa tgtaaaaatg
      541 tttccaaata atgatcctag aaatattccg aaaagggatc gtttgccgag aagcagaaca
      601 gcggagagtg caaaacctgc agcgggaaaa ataggcgagg cgtttgttcc tggaatggcg
      661 agtatcagtc ctgattttga gagaaggaag tacacaagac cagtgaacag aattgcagca
      721 gaaaatctag tcttcagata aatgaaaaat gactgcacag tttattttgg aataaagaag
      781 atcagatgca agctgttttt ttaataagct ttgtaccgat acttatcgat gaggatttat
      841 ttctccagaa ttttaaaaaa ggaaatattt ttccttaatt tttcggaatt tttcttcagg
      901 aggtcggcag ttgcagacag ttcttcggaa acttctgagt tttcctgaga agtttgagcg
      961 atttggatca ttgcagtatt tatctggctg attccatttt tttgttcttc cgatgctgtg
     1021 gaaatttcat ttacaagttc tgctgtttcc tttatgtccg gaagaatttc tttgactagt
     1081 ctttccgcgt tttcagccgt cataacactg tttctggcca actcctgaat ttcttttgca
     1141 gcaatgatgc tctcatcagc aagttttttt acttcagttg cgactacgga gaaaccaagt
     1201 ccatgatccc ctgctcttac tgattcaatg gatgcattca aagcgagcag atttgtctgc
     1261 gcagtaattt tctctatgat ttttgtcttt gccgcaattg ttttcattgc ggtcaaagta
     1321 tcgctcattg ctctttccac tgtggctgct ttattgaatg cagagattgc gatcgattca
     1381 gcgtattttg tattcttatt attatgaatc accgcagatg taattgtttc aatcgctgcg
     1441 gctgattctt ctgcattcgc ggcctgctga cttgcgctgg agctcagttt gtcggtcact
     1501 gaccagagtt catctgtgct gccggcaaga tcattttcaa tgtgaatcag ttccagaatc
     1561 attttttcca taattccaat cgtagtgttg aatgagacct gcagtaaatt aaactctccg
     1621 ttgtaagttc cttttaccct tgttgacaag ttccctcttg atacttcctc agaaattgaa
     1681 ataacttcct gaatcggaag aatgactgca tccagtgttc tgttgaaccc atccagaagt
     1741 ttcttataat ctccctgata tacggatgta tcagctctca aatccagttt tcctgccgtt
     1801 gcagagtccg atattgaatt cagatcaata atcagaccgt tgatagtgct gaccattctt
     1861 tcaaatgatt ttcctatttt tccgatctca tcattttttt ctgtattgat ctttactttc
     1921 aagtctcccg aactgatctt ctctatgctt tctgtgagtt tctgcagaga atcagtaata
     1981 tactttatta aatatgcaac tataacgatt gatataataa aggaaataaa aaatataatg
     2041 acggaagcag cctttgtgtt ttctattgta ctgtgaataa tgccggtatt ttcttcaaga
     2101 atcgatttct gaaaatctgc atattccgca attgtcattg caaagtagat ctgagtcatt
     2161 gaattttttg ccttggagtt gattaaagca atagcttctt cttttttatt attttccact
     2221 gcttttaaga tcaggtcaat ctgaacagac ataagattgt ttttttcaat gagtatgcct
     2281 gtaagttctt taccctttgc tgtatttgaa tgtgtgttca gatagtttag ctgctttgga
     2341 atctcagtat tcatcagctg gattaccgta tttttttcct tcagcagatc ttcaggattg
     2401 gtcatgatga ctaaatttct gactgatctt gcagtctgct gaaagttttt taacagttcg
     2461 tagctcgtaa acagttttgg atttctgtca taaattatat tatccataaa tacattcatt
     2521 ctgtgtatat gaaaaa
//
LOCUS       DAOQGJ010000397         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26110_length_2534_cov_3.891892, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000397 DAOQGJ010000000
VERSION     DAOQGJ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26110_length_2534_cov_3.891892"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1415
                     /locus_tag="PK683_07400"
     CDS             135..1415
                     /locus_tag="PK683_07400"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNH08311.1"
                     /translation="MMSIEFLRFLKPEEVRKAASEFGTPIYVYSEEEIEKRCKDALSF
                     PAEYGLTARYAMKANPNSSILRIMKKNGIHIDASSEYEVERAILAGFAPNEILLTSQE
                     IPEKERLKKIVQRGTEYNACSLQQLEIYGSLFPDSSVSIRINPGLGSGSTKKTDVGGV
                     QSSFGIWHELIQEVQRIKVKYNLKIRRIHTHIGSGSDPEVWKAVSHYTLEYAGIFHEA
                     SIVNLGGGFKVGRMLDEKSTDFQSIGEPVRKEFIKFAERTGRKLHLEIEPGTYMSAGC
                     GAVIAKVMDRVNTGSKGYKFIKLNTGMDINTRPSLYGARHPLVTVTAETEAKRENEKY
                     IVVGHCCETGDVFTQKEGGEAEEREMERAEIGDYVVMEGCGAYCAGMSAKNYNSYPES
                     AEVLLSKDRKFYLIRARQTLEQIMKNEKVPDYLK"
     gene            complement(1479..>2534)
                     /locus_tag="PK683_07405"
     CDS             complement(1479..>2534)
                     /locus_tag="PK683_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08312.1"
                     /translation="SLTPVQKQYVDNANISGHTLLSIINDILDFSKIEAGMMNLEIIR
                     TDIGELLRNSIDLAKFLAGKKNLKILLDADSEMPQFASVDPIRLKQILANLLGNAVKF
                     TEQGEVVLKVVFEHIGPSKGRFKISVRDTGIGISDDQKEKLFKAFSQADESTTRKFGG
                     TGLGLVISDMIAQKMGSKIHIESGVGTGSIFLFDLIADVENVKSQERTAQDFPKSIFN
                     DEKTVLPELKILIAEDVPLNMALVKAVIRKMNPKAILLEAVNGAAAVEQYKKESPDLI
                     LMDVQMPVMDGLDAARAIRLLESESGKYVPITALTAGVFEEEKARCIEAGMDSVLVKP
                     IEPEKLKEVLVRISHAK"
BASE COUNT          758 a          559 c          539 g          678 t
ORIGIN      
        1 agcgaaatcg gggacgtcag tgccgtggcg caaatgtttg accgtctgcg ccagtatttc
       61 cagatgagta atttttcctg ccatcctacg tcgaaaattt gcctttcaaa aaaataggca
      121 aggaaaaact tttgattatg tctatagagt ttctacgttt tttaaagccg gaagaagtaa
      181 ggaaagcagc ttctgaattc ggcactccga tctacgttta ttcagaagaa gaaatagaaa
      241 aaagatgcaa ggatgcactt tcttttcctg ctgaatacgg actgacagcc agatatgcaa
      301 tgaaggcaaa tccgaattcc tccatcctga gaataatgaa aaagaacggc atccatattg
      361 atgccagctc tgaatatgaa gtggaaagag caatacttgc tggatttgct ccgaatgaaa
      421 ttcttctcac ttcccaggaa attcctgaaa aagaaagact gaaaaagatt gtgcagagag
      481 gaacggaata caatgcctgc tcactgcagc agctcgaaat ttatggatcg cttttcccgg
      541 attcctcagt ttccatcaga ataaatccag gtctgggatc aggctctacg aaaaaaacgg
      601 acgtgggagg agttcagtcc tctttcggaa tctggcacga actgatccag gaagtccaga
      661 gaatcaaggt caaatacaat ctgaaaatca gaaggatcca tactcatatc ggttcaggaa
      721 gcgatccgga agtatggaag gcagtctctc attatacact tgagtatgct ggaatattcc
      781 atgaagcatc gattgtcaat ctcggaggcg gattcaaagt gggaaggatg ctagacgaga
      841 aatctactga ttttcagtcc attggcgaac ctgtccgcaa ggaattcatc aagtttgcag
      901 aaagaacagg aagaaaactt catctcgaaa ttgagccggg cacttatatg agcgcaggat
      961 gcggggctgt catcgccaaa gtcatggaca gggtgaacac cggctccaaa ggatataaat
     1021 tcattaaact gaataccggt atggacatca ataccagacc ttctctgtac ggagcaagac
     1081 atccgcttgt cactgtaact gcagaaacag aagcaaaaag agaaaacgaa aagtacatcg
     1141 tggtcggtca ctgctgtgag acaggggatg tattcacgca gaaggaaggc ggagaagcag
     1201 aagaaagaga aatggaaaga gctgaaatcg gagactacgt tgtaatggaa ggatgcggag
     1261 cctactgtgc agggatgtct gcaaaaaact acaattccta tcctgaatcg gcagaagttc
     1321 ttctatccaa ggacagaaaa ttctatctga tccgtgcaag acagacactt gaacagatta
     1381 tgaaaaatga aaaggtaccg gactatctga aatgaaagta ttgcagagac agtctacgta
     1441 ctcgtctctg cgttttcctt aatgaaatat atagcaattt attttgcatg actgattctt
     1501 actaaaacct ctttcagttt ttccggttca ataggcttga caagtacact gtccataccg
     1561 gcctcaatgc atctggcttt ctcttcttca aaaacacctg cagtaagtgc ggtaatcgga
     1621 acatattttc cgctttccga ttccagcagt cgaatcgccc ttgctgcatc caacccatcc
     1681 atgacaggca tctgcacatc catcaggatc agatctggtg actctttttt gtactgttct
     1741 actgcggcag ctccattcac tgcctctaaa agtatagcct ttggattcat ttttctgatg
     1801 actgccttga caagtgccat atttaaggga acatcttcag caatgagaat tttcagttca
     1861 ggcaggacag ttttttcatc attgaagatg ctcttcggga aatcctgtgc agtcctctcc
     1921 tgactcttta cattctcaac atctgcaatc agatcaaaga ggaaaatgct ccctgttccg
     1981 accccgcttt ctatatgaat tttactcccc atcttctgag caatcatatc ggaaataacg
     2041 agtccaagac ctgtccctcc aaatttgcgt gtagtcgatt catcagcctg cgaaaaagcc
     2101 ttgaacagtt tttctttctg atcgtccgaa atgcctatgc ctgtatcgcg cacagagatt
     2161 ttgaacctgc cctttgacgg tccaatatgt tcaaaaacaa ccttcaagac gacctctccc
     2221 tgctcagtaa actttactgc attgccaagc agatttgcca gtatctgttt cagacgaatg
     2281 ggatccaccg aagcaaactg aggcatttca ctgtctgcat ccagcagtat tttcagattc
     2341 tttttccctg ctaaaaattt tgccaaatca atgctgttcc taaggagttc tccgatgtca
     2401 gttctgatga tttcgagatt catcattcca gcctctatct tcgaaaagtc cagaatgtca
     2461 ttgatgatgc tgagaagagt atgtccggat atattggcat tgtcaacata ctgtttctga
     2521 acaggtgtca gtga
//
LOCUS       DAOQGJ010000398         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26160_length_2532_cov_2.448526, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000398 DAOQGJ010000000
VERSION     DAOQGJ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26160_length_2532_cov_2.448526"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="PK683_07410"
     CDS             <1..137
                     /locus_tag="PK683_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08313.1"
                     /translation="YATVLRSATQGKAEFAMEFSRYLQVPKNIADELVKKYKVKQDED
                     "
     gene            408..797
                     /gene="speD"
                     /locus_tag="PK683_07415"
     CDS             408..797
                     /gene="speD"
                     /locus_tag="PK683_07415"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNH08314.1"
                     /translation="MKALGNHVIAEFYDCDLQIIRSQSNIEDIMIEAVQISGAEIVKP
                     VFHTFSPHGVSGMVIVAESHFSIHTWPEYGYCAVDIFTCGNTIDNGKALEYLRKELKA
                     KSLSAVEMKRGIIDLEKDFPHKPESPA"
     gene            1142..>2532
                     /locus_tag="PK683_07420"
     CDS             1142..>2532
                     /locus_tag="PK683_07420"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNH08315.1"
                     /translation="MKRFFFNYIVIIMLLFSSDGCMLSNFFECKSADCRGKSDRSLSF
                     AGILQASSCALNAVGSSGGTQDDENMYKSFTGRIADSEWNSTYIRKILHAFAFGGPAS
                     DAQIDEWVKMGPSKAIIKILNADPVNSGLNVPADGKSGAVSANSSIYCLSSSFANGSS
                     VFPPGNSGKLSLTDTESTGKVYSMMVMLRGLNPVRHVLGFMESNNHLSTNSKLVGPKP
                     QARLYDETMNLIASGSGYEKVLSNAAVSPAMAIQYNHRKNVFKNAAFSGNEDFAREFH
                     QLFFGVLGTGTTTGDITQSTASFQTHETVTVPETAKALTDIRVGGLSNSDDINLDTAV
                     YATEYHLDRAVKIYGSDVTGANAKEKIENASKLAITNSESLNNLPLKIIRFLADDNLD
                     ENDITAASPTAAQLLIQSKAALIRTIWKDISDKNLLLFLRRYALSTAFSNSTRIKYRT
                     SAERIFTISNLVTL"
BASE COUNT          799 a          527 c          537 g          669 t
ORIGIN      
        1 gttacgcaac tgtcctgcgt tctgctactc agggaaaggc agaatttgcg atggaatttt
       61 cgagatatct gcaggtgcct aaaaatatcg ctgatgaact cgtcaaaaaa tacaaggtca
      121 aacaggacga agattaatct ccccttccct tgttttaaga aaccacagca gttctgctgt
      181 ggtttttttt taagcagccg tttcacagaa attttccgct atgaaatctt ttcaatcaca
      241 attaaatctg actgaatgat gaacggaaat acctcattaa tttcatccaa taaagcagtt
      301 ctgtctcgat gtctgaatca gtacagtgaa gtgaaaagga aagacttcat tttctcgact
      361 ggccgaaaaa aagctgattg cagactctct cgaaaaaata aacagccgtg aaagcattag
      421 gaaatcatgt aattgcagaa ttttacgact gcgatcttca gatcattcga agtcagtcaa
      481 atatagaaga tattatgatt gaggcagtcc agatttcagg agcagagata gtaaaacctg
      541 tatttcatac tttcagtccg cacggagtca gtggaatggt cattgtggca gagtcgcact
      601 tcagcattca tacctggccg gagtacggat actgtgcggt ggacatattc acctgcggaa
      661 acacgataga caacggcaaa gcactggaat atctcagaaa ggaactgaag gcaaaaagtc
      721 tgtctgcagt ggaaatgaaa agaggcatca ttgatctcga aaaggatttt cctcataaac
      781 cggaaagtcc cgcttaactg aactgcgcag accgcgctct agcggtttca agtttgcaag
      841 cgaaaggcgg ctgaaggcgc aaggcaagga gtaattgtat gttttggaag aatgtcttca
      901 tttccagttt tcaaacaggc aaataaagag aggctttcag ttaaattata ttggatttga
      961 aactaagaat cataacctaa actcagcttt ttacgattcc gtaaaagctt ggacatcatt
     1021 ttcttcatcc gtatcagaaa aagcggcttt tttactttct ttattcagaa acaaactctt
     1081 tggtgtaaat tatcaggatt ttcatattga tctgaataag gatcaatggg gggaaagaac
     1141 attgaagaga ttttttttta attacattgt cattattatg cttcttttca gttcagacgg
     1201 atgcatgctt tcaaattttt ttgaatgcaa aagtgcagac tgcagaggga aatcagacag
     1261 gtcactgtca tttgcaggga tactgcaggc atcttcctgc gcattgaacg cagtcggaag
     1321 ttcaggagga actcaggatg acgagaatat gtacaagtca tttacaggaa gaattgctga
     1381 ttccgaatgg aactccacct atatcagaaa aatacttcat gcattcgcgt tcggcggtcc
     1441 tgcttcagat gctcagatag atgaatgggt gaaaatgggg ccttccaagg caatcattaa
     1501 aattctgaat gcagacccgg taaactccgg cctgaatgtt cctgcagacg gaaaatccgg
     1561 ggctgtctct gcaaattctt ccatctactg cctgagttct tcatttgcaa atggaagctc
     1621 agtttttcct ccaggcaatt ccggcaaact gagtcttacg gatactgaat ccacaggaaa
     1681 agtatattca atgatggtaa tgctcagggg tctgaatccg gtccgacatg ttcttggatt
     1741 catggaatcg aacaatcatc tttctaccaa ctcaaaactt gtaggaccca aacctcaggc
     1801 cagactttac gatgaaacaa tgaatctaat cgcatcagga agcggatatg aaaaggttct
     1861 ttccaatgca gcagtttcac ctgccatggc gattcagtat aaccacagaa aaaacgtttt
     1921 caaaaatgca gccttctcag gaaatgaaga ctttgccaga gaattccacc agctgttttt
     1981 cggagttctg ggaacaggga caacgacagg agacatcact cagtccacgg caagctttca
     2041 gactcatgag actgtaactg ttcctgaaac agcaaaagca ctcacagata ttcgggtagg
     2101 aggtctttcg aattcagatg acatcaacct ggatactgca gtgtatgcga ctgaatatca
     2161 tttggacaga gcagtaaaaa tatacggatc cgatgtgacc ggtgcaaacg caaaggaaaa
     2221 aatagaaaat gcatcaaagc ttgcaatcac caactctgaa agtctgaata atcttcctct
     2281 caaaatcatt cgattccttg cagacgacaa tctggatgaa aacgacatca ctgcggccag
     2341 cccgactgca gcgcagcttc tgattcagag caaggccgct cttatcagga caatatggaa
     2401 agatatttcc gacaaaaacc ttcttctctt tctcagaaga tacgccttgt caaccgcctt
     2461 ttccaacagc acaaggataa agtacaggac aagtgccgaa agaattttta caatcagcaa
     2521 tctggtgacg ct
//
LOCUS       DAOQGJ010000399         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26227_length_2528_cov_2.663162, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000399 DAOQGJ010000000
VERSION     DAOQGJ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26227_length_2528_cov_2.663162"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /locus_tag="PK683_07425"
     CDS             <1..1579
                     /locus_tag="PK683_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNH08316.1"
                     /translation="HISADNKIHAIAYMEDAENETSDITEEKYVFIPDIPKPEFSVSP
                     GIYTDFISLKIRSLVSGVSIRYTLDGSTPTSSNGLLIPASSAWTAGEGSVIEIQKTVT
                     VKAVAYSDHKTSETLSGIFTISVPSVPEFSIPGGEYANPQTLSLNAPSGQEIWYTLNG
                     SVPSPVSAIKYGSPIIIPGNTKVSAISCFPNTSNCTSAVSANYVILSRLAPPVFSPVP
                     GEFSSAQSVTLSSNEPNAKIYYTLDGSMPVPGVSPMYSSPILIQSTANIRTLAVMAGY
                     TDSAESSGIYSIRMFSDAHISQLVNLANVSYGQLAGEGLTSEVQRAQSLVRANPSLIS
                     NSSLRTKFLNQNGALAASELCTVISRYLYLYAFSKIYPERLQNLPSGFAEFYVYAVNK
                     GYVITDSGGVTFNWTADGYNMVKDFTVPLYFSSWIKYRTDAGFGTDGSLLSFVSGKEG
                     RLFFLSDGPDGNGATHNFLAVMHPVSGFKMLDTYHHAWTGKGFYENANVSSYYAVRFG
                     ASGTRWLHIVNGYLQTK"
     gene            complement(1770..>2528)
                     /locus_tag="PK683_07430"
     CDS             complement(1770..>2528)
                     /locus_tag="PK683_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08317.1"
                     /translation="GGFGGNEMILFRPNHIIAGFFAEEGDYFGARHITRLRIVWQKWG
                     AGRPEGDLILSERYGRGSFTRGTVETEIIAPFGRIVSLLTGTHSCHSDGNCFLSTLSI
                     QTASTIPEKDSSGLIKTAGNVNYDFEIQVISGFNEGKKYRGSFSYDSSLLKGRGEETI
                     EVSDIQFTYENDAYIRSSFDSVPTAVFQDGKFKDLVLVGGPTTKRFGLNAGFDRRQFG
                     RPSEAFILEGKSYFGYLDAATYVEGAGKVIYIKK"
BASE COUNT          643 a          598 c          556 g          731 t
ORIGIN      
        1 tcatatatct gcagataata aaattcatgc cattgcatat atggaagacg cggaaaatga
       61 gacatcagac atcactgaag aaaaatacgt ctttattccg gacattccga agccggaatt
      121 cagtgtcagt cctgggatat atactgattt tatttcattg aaaatacgtt ctcttgtatc
      181 tggagtcagc atccgctata ctctggacgg gagcactccg acatcttcaa acgggctgct
      241 gatccctgca tcctctgcat ggacggcggg cgaggggagc gtaattgaga ttcagaagac
      301 tgtgacagta aaggctgtcg cttattcaga tcataaaact tctgagactc tttcaggcat
      361 ctttacaata tcagtcccgt ctgtccctga gttcagtatt ccaggaggcg aatatgcaaa
      421 ccctcagact ctgagtctga atgctccttc ggggcaggag atctggtata ctctgaacgg
      481 atcggttcct tctccggtat ctgcaatcaa gtacggttca ccgatcatta ttcccggaaa
      541 tacaaaggtt tcagcaattt cctgttttcc gaatacatcc aactgcacct ctgctgtatc
      601 cgcaaattat gtcattctga gcagactggc tcctccggta ttctctcctg ttccgggtga
      661 attctcttct gcacagtcgg tgactctgag ttcaaatgaa ccgaatgcaa agatttatta
      721 tacactcgac ggaagtatgc ctgttcctgg agtaagtcct atgtattctt ctccgattct
      781 cattcagtct acagccaata ttcgaactct ggctgtcatg gccggatata ccgattcggc
      841 agaatcctcc ggaatttaca gcatccggat gttcagtgat gcgcacatca gtcagcttgt
      901 caatctggcc aacgtctctt acggacagct ggcaggagag ggactcacct cggaagttca
      961 gagggcacag agtcttgtga gagcaaaccc ttcccttatc tcaaactctt ctcttcggac
     1021 aaaatttctg aatcagaacg gggctcttgc ggcaagcgaa ctctgcacag tgatttcgag
     1081 atatctttat ctgtatgcgt tttccaaaat ctatccggag agactgcaga atcttccttc
     1141 cggatttgcc gaattctacg tatacgctgt aaataagggg tatgtcatta ccgattcagg
     1201 cggagtgact ttcaactgga ctgcagacgg atacaatatg gtaaaggatt ttacagttcc
     1261 tctgtacttt tccagctgga taaagtacag gacggatgcc ggattcggaa cggacgggtc
     1321 gcttctgagt tttgtttcgg gaaaagaggg gagacttttt ttcttaagcg acggccctga
     1381 cggaaacggt gccactcata attttcttgc ggtaatgcat cctgtctcag ggttcaagat
     1441 gctggacaca tatcatcatg cctggactgg aaaggggttt tatgaaaatg cgaacgtcag
     1501 ttcctattat gctgtccgtt tcggtgcatc aggaacgcga tggctgcata ttgtaaacgg
     1561 gtatctgcag acaaaataga cgtttccaat acatatatgg gactgtgtaa aagcaggtta
     1621 ccgagtgatt ttcaccgccc acctcgatac gaaaaaccct gaaagtattg ggtttttcat
     1681 acctcgacaa gctcggcaca tcgctcggtg accggcggtg aaaattgtat cgaggtatgc
     1741 ttttacaaaa cctctgcggc tgctgagcct tattttttaa tataaattac ctttcctgca
     1801 ccttccacat atgttgcggc gtccagatat ccgaaataac tttttccttc cagaatgaaa
     1861 gcctctgaag gtctgccgaa ctgtcttctg tcaaagccgg cattcagccc gaatcttttt
     1921 gtagtgggtc ctcctacaag taccagatcc ttgaacttgc cgtcctgaaa aaccgctgtc
     1981 gggacagaat caaaggagga tctgatgtag gcgtcatttt catatgtgaa ctgaatgtct
     2041 gaaacttcta ttgtctcttc tccccgtccc ttcagaaggg aagagtcata actgaaactg
     2101 cctctgtatt ttttgccttc attgaatcct gaaatcacct ggatttcgaa atcataattt
     2161 acattgcctg cagtttttat aagaccggaa gaatcttttt ccggaatcgt ggatgcagtc
     2221 tgaatcgaca atgtggaaag aaagcagttc ccgtcactgt ggcaggaatg tgttcctgtg
     2281 agaagcgaga caattcttcc gaacggggcg atgatttcag tttctacagt tcctcttgta
     2341 aaacttcctc tgccgtatct ttctgaaaga ataagatcgc cttcaggtct tcctgctccc
     2401 catttctgcc atacgattct gagtctcgta atatgtcttg ctccgaaata gtctccttct
     2461 tctgcaaaga aacctgcgat tatatgattc ggcctgaaaa ggatcatctc atttccgccg
     2521 aaccctcc
//
LOCUS       DAOQGJ010000400         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26242_length_2527_cov_3.188916, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000400 DAOQGJ010000000
VERSION     DAOQGJ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26242_length_2527_cov_3.188916"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PK683_07435"
     CDS             complement(<1..835)
                     /locus_tag="PK683_07435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH08318.1"
                     /translation="MKFGNPTVLFLIVFLDMLGFSIIFPIFPKILNFYLQNGDPLAAE
                     FLKVFSSFSSKETGNTVLLGGILGSIYAVLQFIFAPVWGSLSDRYGRKPLMVWTGFGN
                     LFGYLIWLFSGSFSLFILSRTVTGAMGGSISVASAAMADATNEKDRTKGMGMIGAGIG
                     LGFVFGPPFGGISSEWNFSFLNNSSNPAAVPAFLSVLIALLCLSSCILFFKETAPFGS
                     QEIRRHPIFSFSAVRGKDIAQVIAVYFIYTFGFSGFEFCLNFYLNDSFQFTPKQIGMS
                     FV"
     gene            complement(913..1035)
                     /locus_tag="PK683_07440"
     CDS             complement(913..1035)
                     /locus_tag="PK683_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08319.1"
                     /translation="MKEFFEKLDITSPSGFLLGTKIPVLSVVFRKNDENGPRVP"
     gene            complement(1032..1253)
                     /locus_tag="PK683_07445"
     CDS             complement(1032..1253)
                     /locus_tag="PK683_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08320.1"
                     /translation="MKLSIESLIDIGIGIIKTNEENLKKTSNEIVSWTEKMKAIGASD
                     DSEKTNLIRKLAGKVSRDFDGLKGNLMRK"
     gene            1355..1993
                     /locus_tag="PK683_07450"
     CDS             1355..1993
                     /locus_tag="PK683_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH08321.1"
                     /translation="MYFKFVLLSLLFLISCSTVGDRLAEEAYSGNLDMVKTLSEKGQK
                     NDARNDKGWTALMAASESGEMLVVKYLLDTEANPNLRNALGDTAMHRATVRGNLEVIR
                     SLHGAGAYPNLRNESGATPLMKAVEEGTPKHKEIISFLVSAGANINDRDFDGNTALAR
                     AVKKQNADMVKFLIGLKADPKAENKFGEPPMLAAKEAKNREIIEILRSAGAK"
     gene            2087..>2527
                     /locus_tag="PK683_07455"
     CDS             2087..>2527
                     /locus_tag="PK683_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08322.1"
                     /translation="MHTKYYLLILSLFVFYSTNLFSQRTLEIPKDDKSKKEKAKKTAK
                     ETLETKAAEKMSEEAKKLETEAGKRLSSLIKADSKKVQVFKGVSVQRFSIREENRLTG
                     DNIELGPDAGLAGSAGVSRFIAGYIQDSFSYSQEDSETLSQYVLF"
BASE COUNT          837 a          530 c          532 g          628 t
ORIGIN      
        1 ttacaaaaga cattccaatc tgcttcggtg taaactgaaa gctgtcatta agataaaaat
       61 tcagacagaa ttcgaagccg gaaaaaccga atgtatatat aaaatatacc gcgatgacct
      121 gagcaatatc cttccccctg actgctgaaa aagaaaatat ggggtgacgg cggatttcct
      181 gtgaaccgaa gggagccgtt tccttaaaga aaagaatgca gctcgaaaga cagagaagcg
      241 cgatcagaac agaaagaaat gcaggaaccg ctgctggatt cgaagaattg ttcaaaaaag
      301 aaaaattcca ttcagatgaa atccctccga aaggaggtcc gaaaacgaaa ccgagaccta
      361 tccctgcacc gatcattccc atccctttgg ttctgtcctt ttcattcgtg gcatcagcca
      421 tggcggccga tgccactgaa atgcttccgc ccatcgctcc ggtaactgtt ctggatagga
      481 taaaaagtga aaaacttccg gagaaaagcc agatcaggta gccgaaaaga tttccaaaac
      541 cggtccatac catcagcggt tttcttccgt atcggtcaga cagagaaccc cagacaggtg
      601 caaatatgaa ctgaagcact gcataaatgc ttcccaaaat ccctccaagc agaactgtat
      661 ttcccgtctc tttcgatgaa aatgaagaga acactttcag aaattcagca gcaagaggat
      721 ctccattctg cagataaaaa ttcaagattt tagggaagat agggaatatg atcgaaaaac
      781 cgagcatatc aaggaatact atcaggaaaa gtacggtcgg atttccaaat ttcataaatc
      841 taaggtaagg aaaaagagat atgcaggaaa agcaattgga atattcagaa agaaagaaat
      901 tctaatgatg aattacggaa ctctcggacc gttttcatca ttcttccgga ataccacgct
      961 gagaacggga atttttgttc caagcagaaa accggaaggc gaagtgatgt ccaatttttc
     1021 aaagaactcc ttcacttcct cattaaattt cctttcagac cgtcgaaatc cctgcttact
     1081 tttccggcaa gtttacggat cagatttgtc ttttcagaat catcggatgc acctattgct
     1141 ttcatttttt ctgtccagct gacaatttca ttggaagttt ttttcaggtt ctcttcattt
     1201 gtcttgataa ttcctattcc gatatcgatc agagattcga ttgaaagttt catagtctgt
     1261 tccgaaatta attgataaat ccactttttt tttcagaccc gttctttcca gaattttttc
     1321 ttgtgagcaa agaggacatt ttcataatct ccctatgtat ttcaaatttg tactcttatc
     1381 ccttcttttt ttgatttcat gttccaccgt cggcgacaga cttgcggaag aagcctacag
     1441 cggcaatctg gatatggtaa aaactctctc agagaaaggc cagaaaaacg atgcaagaaa
     1501 cgacaagggc tggaccgccc tgatggcagc atctgaatcc ggtgaaatgc tcgtagtcaa
     1561 atacctgctg gatacggaag caaacccgaa tctgaggaat gccctgggag acacggcaat
     1621 gcaccgtgcg acagttcggg gaaatctgga agtcatcagg tcgcttcacg gagcaggagc
     1681 atatccgaat ctcagaaatg aatcgggggc tacacctctg atgaaagctg tggaagaagg
     1741 aacgccgaaa cataaggaaa tcatttcctt cctcgtcagc gcaggagcaa atataaacga
     1801 cagggacttt gacggaaaca ctgcactcgc aagggcagta aaaaaacaga atgcagatat
     1861 ggtaaaattt ctcattggtt taaaggcgga tcccaaggct gaaaataaat tcggagaacc
     1921 tccaatgctc gctgcgaagg aagcaaaaaa cagagagatc atagaaatac tgagatcggc
     1981 aggggcaaaa taactcttca gattttacat cctgatatta gtaatttctt gcaatttcta
     2041 atctgaaagc aaatctgaca aatgactcta aggagaatcc tatttcatgc atactaaata
     2101 ctacctcttg atcctgagct tatttgtatt ttacagtaca aatctgtttt ctcagagaac
     2161 tttagaaatc ccgaaggatg acaaaagcaa aaaagaaaaa gcaaagaaga cagcaaagga
     2221 aacactcgaa acaaaagcag ctgaaaaaat gagcgaagag gctaagaaac tcgaaacaga
     2281 agctggaaaa aggctctctt ctctcattaa ggctgattct aaaaaggttc aggttttcaa
     2341 aggagtcagt gttcagagat tcagtatcag ggaagagaac agactgacgg gagacaatat
     2401 tgaacttgga ccggacgcag ggcttgcagg aagtgcaggc gtatccagat tcatcgccgg
     2461 ttacatacag gacagcttct cctattccca ggaagattca gagactttat ctcagtatgt
     2521 tctcttt
//
LOCUS       DAOQGJ010000401         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26243_length_2527_cov_2.880259, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000401 DAOQGJ010000000
VERSION     DAOQGJ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26243_length_2527_cov_2.880259"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK683_07460"
     CDS             <1..195
                     /locus_tag="PK683_07460"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HNH08323.1"
                     /translation="ASFSQEDPRIWNNYKHKKYDTLLLEIESDFRQGINTTGVLNFLI
                     QEKDLINRKFSRVMYTGDCG"
     gene            complement(199..1431)
                     /locus_tag="PK683_07465"
     CDS             complement(199..1431)
                     /locus_tag="PK683_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH08324.1"
                     /translation="MFLFIFVSIHAETKKIHPKTAVGVSEKPKHTRSVTAFRSLPAQT
                     VKISGLKAVMLAGEVDGVNGPSTKSYTDNLKQLAKALKDRNVKVVEIYSPTTEEKIKT
                     ELKDANFVFYAGHGIGGADPPSYKATGSDGGMLILADVWTGGSDVKEWEVKKGAIVFF
                     MGACFTAGNAGSDIGKINAEEAKRRISQYSEPYLNSRFGGYYAAWSPYELEEVIANLF
                     AGKTLGESYGGKDSEYGKVNKISHPKSSGNSLWFYEEKGDRKAFSFSFAGNPNQTLTT
                     LFSSGSEDSKPVSENNDTPKPIDEKQNAVLIKAIYKGDDAAALQAINDGADPNAKHKE
                     WSALLLASHFNRENIAKRLIQRKADVNYELKGWSSLRIAEKHNYTGIADALKSAGAKA
                     SSRFIRGVNPVPEIAPAP"
     gene            1959..>2527
                     /locus_tag="PK683_07470"
     CDS             1959..>2527
                     /locus_tag="PK683_07470"
                     /inference="COORDINATES: protein motif:HMM:NF020035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08325.1"
                     /translation="MNHLSKGFEQCEANLVHTGLIQGFGAVCRVSLKNETITHASENF
                     EELTGVKNPLGQKMNSVFGTEMTARLTGLSARTAFSERWKALTVFGRAESDSLTVEIE
                     PASDLSQRYERFWLTKITDKNDADHKILNFISSVSGFERIMLYEFLSDGTGCVTGEIY
                     HGEGDRYLGLRYPATDIPLTARNLYIQNS"
BASE COUNT          695 a          526 c          540 g          766 t
ORIGIN      
        1 gccagcttct cccaggaaga tcctaggata tggaataatt ataaacataa aaaatatgat
       61 acccttcttc tggaaattga atctgatttc cggcagggaa tcaatacgac aggcgtactg
      121 aatttcctga ttcaggagaa ggatctcata aacagaaagt tttccagggt aatgtacacc
      181 ggagactgcg gctgagattt aaggagccgg agcgatttca gggaccgggt ttactcctct
      241 gataaatctg ctggatgctt ttgctcctgc cgatttcaat gcatctgcaa ttcctgtata
      301 attgtgcttt tctgcaatcc tgagtgaact ccatcctttc agttcgtaat tcacatcagc
      361 ctttctctgg atcagacgtt tcgcaatgtt ctccctgttg aaatgcgagg caagaagaag
      421 agccgaccat tctttgtgtt ttgcgttcgg atctgctccg tcattgattg cctgaagtgc
      481 ggctgcatca tctcctttat agattgcttt aatgagcaca gcattctgtt tttcatcaat
      541 cggtttagga gtatcattat tttcagatac aggtttgctg tcttctgaac ctgatgagaa
      601 gagggttgta agtgtctgat tcgggttacc tgcaaatgaa aaactgaatg cctttctgtc
      661 tcccttttct tcatagaacc aaagactgtt tccggatgat ttaggatgag aaatcttatt
      721 cactttacca tactcagagt ctttacctcc gtaagattct cctaatgtct ttcctgcaaa
      781 aaggtttgca atgacctctt ccagttcata aggggaccat gctgcataat atcctccgaa
      841 cctggaattc aagtaaggtt cagaatactg tgaaatcctt ctctttgctt cttccgcatt
      901 gatttttcca atatcacttc ctgcattgcc tgcagtgaaa caggctccca taaaaaatac
      961 gatggctcct tttttgactt cccattcctt tacatcagaa ccgcctgtcc atacatcggc
     1021 tagaatcagc attcctccgt cagaacctgt cgccttataa gaaggaggat cagcaccgcc
     1081 tatcccgtgt cccgcataaa atacaaaatt tgcatctttc agctcagttt taattttctc
     1141 ttcagttgtt ggggaataaa tttccactac tttgacattc ctgtctttca acgcctttgc
     1201 gagctgcttg agattgtctg tataactttt tgtagatgga ccgttgactc cgtctacttc
     1261 acctgcaagc atcactgcct tcagcccgga aattttgact gtctgtgccg ggagagatct
     1321 gaatgcagtg actgaccgag tatgttttgg tttttctgaa acaccgacgg cagttttcgg
     1381 atggatcttc tttgtctctg catgaataga gacaaaaata aaaaggaaca taaaattcat
     1441 cagtatgatt ttcatgcaga ttatttttca ctttgtgaaa ggatggtcaa gcaaaaaagg
     1501 aagctgcccg tcggatgaac gggaatgctg tttctgcaag aacgtagaac tccagagcac
     1561 cggattatga atgaaaatcc tgaaagtata tctcagattt ctgatcggac taggcaggat
     1621 tgcaaattca ggtataacga tgccgatatt ttaaaaaata tatgttataa ctattttaaa
     1681 ttactgaata tatacgatgt atctttcgga aagcaataga attcggcatt ttcaggcctg
     1741 cgatgaatca gtaaaaacag ccgcttcgcc gcttcgctgc ttccatactt gcagacttta
     1801 gacagacatg aagagcagtt ctgactgttc tctgaccagg cagatctttg taaagaatat
     1861 tcatcggcac ctgaaattgt aaaagactgc agacagttct gctctgcttg acagtcagga
     1921 acggactcgt atttttgcag cagagatatt cagagggaat gaatcatttg agcaagggtt
     1981 ttgagcaatg tgaggcaaat ttagtacata ctggtttaat tcaggggttc ggagctgtat
     2041 gcagagtatc cctaaagaat gaaacaatca cccatgcttc cgaaaatttt gaggaactga
     2101 ccggagtgaa aaaccctctt gggcagaaga tgaattctgt tttcgggact gaaatgactg
     2161 cccgtctgac aggactttct gcgcggacgg cattcagtga aagatggaag gcattgactg
     2221 ttttcggcag ggctgagtca gactcactga ctgttgaaat tgaacctgct tctgatttga
     2281 gccagagata cgaaagattc tggctgacaa aaattactga taaaaatgat gcggatcata
     2341 agattctgaa tttcatttca tctgtcagcg gttttgagag aataatgctc tatgaatttc
     2401 tttctgacgg aaccggctgt gtgacaggcg aaatttatca cggagagggt gacaggtatc
     2461 ttgggcttcg gtatcctgca acagatatac ccttgactgc aaggaatctg tatatccaga
     2521 attcata
//
LOCUS       DAOQGJ010000402         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26261_length_2526_cov_3.279644, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000402 DAOQGJ010000000
VERSION     DAOQGJ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26261_length_2526_cov_3.279644"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..2526
                     /gene="rpoB"
                     /locus_tag="PK683_07475"
                     /pseudo
     CDS             1..2526
                     /gene="rpoB"
                     /locus_tag="PK683_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214121.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT          839 a          491 c          564 g          632 t
ORIGIN      
        1 ttatcttcca aatttcatcc aaatccaaaa aaagtgattt gattggtttc ttcagccaga
       61 agtaaaagat ccaaccaaga gaaagaatca gggtctggag gcagttttcc gggaaacttt
      121 tccaattgaa agcccaaaca gtgatgtgat aatggagtac agccattatc tgttaggtga
      181 aatcaaaaga gagcctcagg aatgcaagga tacagattct tcctatgcag ttcctctgaa
      241 ggctgtcata cgtcttatta ttaaagaaac cattcagatc agggaagagg tcgtctatat
      301 gggcgacgtt cctgtaatga ctgaacaggg tacatttatc attaacgggg ctgaaagagt
      361 ggttgtaagt cagctccaca gatctccagg tatcttcttt tctcaggatc agatgaaagg
      421 aacgttttct gcaagggtca tcccttacag aggttcctgg ctcgagttcg aaatcgataa
      481 caagggcatc ctcgttgcga aaatcgacag gaagaaaaaa ttcccggcaa cacttcttct
      541 gaaagcattg ggaattggaa gcaatgaaga ggttctcaga cttttttatt cttccacaaa
      601 ggcaaaactt cacaatgcaa atcctaaaga actcaagaag atcatcggca gaagaacaat
      661 tgcagatatt gtaaacagtg aaactggcga ggtaatgctc gaagccggtt ccaaaatcaa
      721 tgaagacaat atcgacattc tgaaggaaat gaaagtcaag gaagtggatc tgattgattt
      781 tccaaaacag aaggacaatc ctacaatcat caactgtctt gaaaaagatg ctgtaaatga
      841 ttatgaggaa gcaatcaaaa aatttcatct gattatgaga ccgggtgaac cttcttcgct
      901 cgaaaatgct caggcagaat tgaaaagact cttcttttcc ccaaagacat ttgatctcgg
      961 gaaagtaggg cgttataaaa tcaacagcaa gtttgaatac cacaagcctg agatttttaa
     1021 agagtgcgag gatagaactc ttcgccctga agacatcata gaaactgtaa gatacctcgt
     1081 aatgctcatt tcggaagtgg aaaactattt ccatgatgac attgaccatt tgggaaacag
     1141 aaggatcaga tcggtagggg aacttttgag caaccagctc aaactcggtt tctccagagt
     1201 ggaaagagtg atcaaggaaa gaatgactgt tcaggaagtg gaacagcaga ctccaaggct
     1261 cctgatttcc atcaaaccga tcacagcagt catcaacgaa tttttcggat catcacagct
     1321 ttctcagttt atggatcaga tcaatccgct cgcagaactg acacataaga gaagactgaa
     1381 tgctctcggc ccaggaggtc tctctcggga cagagcaggg tttgaagtgc gtgacgttca
     1441 ttacagtcat tatggccgta tgtgtccgat tgaaactcct gaaggaccga atatcggtct
     1501 gattctttct atgtcttcct atgccagagt caacgactat ggattcctgg aaacacctta
     1561 cagacttgta aaatccggaa gagtgcataa aaaagaaatc gaatatctca cagcagacag
     1621 agaagaatac cattatattg cacaggcatc tgcaacgatc gatgacaagg gtgatttcaa
     1681 ggacaaactg attcctacac gtcacagatc tgatttccct ttcagaagtc cgaatgaaat
     1741 ccagtatatg gatattgcac cgatgcaggt cgtttctgtt tcgactgcac tgattccttt
     1801 cctggagcat gatgatgcga accgtgctct gatgggttcg aatatgcagc gtcaggcagt
     1861 tcctcttctt gtagaagaag ctccttttgt aggaacagga atggaagaaa gagcagcata
     1921 tgattccagg atctgtgtaa ttgcaaaaag aaacggagtt gtcgtaaagg tagatgcaag
     1981 caaaatctgc gtcaaggaag aaggatcgaa agaactgata gaataccatc ttctgaaata
     2041 caagaagaca aaccagggaa cctgcttcaa tcagaagccg gttgcaaaag tcatcatggc
     2101 ttctgatgac ggaaaaatca ccaagattac aaaagagaag atcgaattca cttctgagac
     2161 tggtgtgaaa tataattatg aactctttga aacttacaga gaattcactc ctctcgtcaa
     2221 agaagggatc catgttacaa aaggaactgc aattgcaggc caggttgtat acggtgaaaa
     2281 acttgatccg aacggcaata tcattgtaaa aggaactgtt ctcgctgacg gtcctgcaat
     2341 tgacaatgga tatttagcat tgggaaagaa tgttctagta ggatttatgc cctgggaagg
     2401 atacaacttc gaggatgcga ttgtcatcag tgaaaacatt gtgaaagatg atgtattcac
     2461 ttcagtccac attgaagaat ttgaaatcca ggcaagagaa acaaaactcg gtcaggaaca
     2521 gattac
//
LOCUS       DAOQGJ010000403         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26264_length_2526_cov_2.902469, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000403 DAOQGJ010000000
VERSION     DAOQGJ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26264_length_2526_cov_2.902469"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            533..2368
                     /locus_tag="PK683_07480"
     CDS             533..2368
                     /locus_tag="PK683_07480"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH08326.1"
                     /translation="MKYMEDPEGKMSFQDVLQSELEKKFRSVESDTPTFGYARSAYWF
                     ILPYRSETDLSDFLGAVEFPILDEVNIFYYNKENRLTEKIFGRKLKFREREIYHRDFL
                     FRMSDFRNSGKIYLRVKTESSLQVPLILYKENRFWETDQTRLAFQFLFIGIMTSMILY
                     NLLLGLSLKDRIYFYYILYVISASLFLAGLNGINYQIFWPDLPGWNKISSPVLLSLTN
                     LGIAVFADSFLKVKDHYIILHRIFMILKAASLTGFFCSFVFSYITVSRLLLSNVLSVS
                     ILSIITSALIAFKGYRPAFIYMISWAVLLFGAFLLALSRFGFIPLNFLTENTAQIGVA
                     LEAILISFALADRIKILQKEKESAQFSALEAHGKAEMLKHDMELAAKIQKSILPNKIP
                     RIEGLEISAWYHPMESVGGDFYDFRENDGNLGFIMADVSGHGVPAALVVSSLKTAFWF
                     QDLNHSNPSELLHSLNQILDGKTGGEFITACYVHLDMKNRILKTGNAGHSALVVFQRE
                     TEMIKCFNPKGAALCIRPNPKFDSLEFPLSSGDRIILYTDGLFEVENFLTEQFSQERI
                     FAILKENSHLSGNEFGEHLLSRVKEWSGGKEKFEDDIALIVVDVK"
BASE COUNT          775 a          525 c          515 g          711 t
ORIGIN      
        1 tctttcattc cgagattcaa ggcagatacg accgtatgtc gcaaaaccct gaaaagcaga
       61 ctttcccgca tctttctgat tctgaatatc aaaaaaagaa ctggacaata atcaaaaaga
      121 aggttcagct gcaaagagga gccaaaaagg agaactgcaa aaatgccttt caataaaagg
      181 aacgaaactg agtttgaaac agcagaaact cacagaaaag agaaactgcc tgcaaacact
      241 aaaaaaactt cagtggaatc gaaacgttct ccggcatccg tttttgattt tcctgccgaa
      301 cagcggcctc acgaatgcaa tgtacgatcc gccggctctt atatgaaaag cagtcatcgg
      361 atctgaatat attatcatga ttttttatgc actaatttca gagtcatttt ctggactttg
      421 cttaaaatgc cggatattct gcattgtctg tacaattatc ctgagtcttc ctctgaattc
      481 ggaaacattg aatattgatg tgtcaaaaac agaaatcagt acaggacgat atatgaaata
      541 tatggaagat cctgaaggga aaatgagttt tcaggatgta cttcagtcgg agctggaaaa
      601 aaaattcagg tctgtcgaaa gcgatactcc cacattcggc tatgcacggt ctgcctactg
      661 gttcattctt ccctatagat cggaaacaga tctttctgac tttctgggag cagttgaatt
      721 tccgatcctg gatgaagtaa atatattcta ttacaataaa gaaaacagac tgacagaaaa
      781 gattttcggc agaaagctca aattcaggga aagagagatc tatcatcggg actttctctt
      841 cagaatgtcc gatttcagaa attcaggaaa aatctatctc agggtgaaga cagaaagttc
      901 acttcaggtt cctctgattc tgtacaaaga aaacagattc tgggaaacgg atcagaccag
      961 actggccttc cagtttcttt tcataggaat catgacttcc atgattcttt acaatctgct
     1021 tcttggactt tccctgaagg acagaatata tttttattat atattatatg taatatcggc
     1081 ttctcttttc ctggcaggac tgaacggaat caactaccag attttctggc cggacctgcc
     1141 tggctggaat aagatttcca gtcctgttct gctctctctc accaacctgg gcattgcagt
     1201 gtttgccgat tcttttctga aagtaaagga tcattacata atattgcata gaattttcat
     1261 gatcctgaag gcagcatctc tcactggatt cttctgttca ttcgttttct cctatatcac
     1321 tgtcagcaga cttcttctgt cgaatgttct ttctgtttcg attctttcaa tcattacatc
     1381 ggcactgatt gcatttaaag gatacagacc tgcattcatc tatatgattt cctgggcagt
     1441 tcttctcttt ggcgcatttc ttcttgcgct gagcagattc ggattcatcc cgctgaattt
     1501 tctgacagaa aatacagccc agatcggagt ggctcttgaa gcgatactca tttcttttgc
     1561 ccttgctgac agaattaaaa ttctgcagaa agaaaaggag tctgctcagt tttcagccct
     1621 tgaagcacac gggaaggcag aaatgctgaa gcatgatatg gaacttgctg caaaaataca
     1681 gaaatctatt ctgcccaata agattccaag aatcgaaggg ctggaaatct cagcctggta
     1741 ccatccgatg gaaagtgtcg gaggtgattt ttacgatttc agagaaaatg acgggaatct
     1801 tggattcatc atggccgacg tttcaggaca cggggtaccc gcagccctcg tagtatcatc
     1861 tctaaagact gcattctggt ttcaggatct gaatcacagc aacccgtcag aacttctgca
     1921 cagcctgaac cagattcttg acggaaagac aggcggcgaa ttcatcactg catgttatgt
     1981 ccatctggat atgaagaaca gaatactgaa gacaggaaat gcaggtcaca gtgctctcgt
     2041 agtatttcag agagaaacag aaatgatcaa gtgcttcaac cccaaaggag ctgctctgtg
     2101 catccgccca aatcccaaat ttgattcatt ggaattcccc ctcagctcag gggaccgaat
     2161 catactgtat acagacggtc tgttcgaagt ggaaaatttc ctcacggaac agttcagtca
     2221 ggagaggata tttgccatac tcaaggaaaa ctctcatctt tccggaaatg aattcggaga
     2281 acatcttctt tcaagagtaa aggaatggtc aggcggaaaa gaaaagtttg aagatgacat
     2341 tgcgctgatt gtagtcgatg taaaataata ataataaaat caactgtata atattttagt
     2401 tttatgcttt ttctctgaat tctgactgca gaatctgcat ggggaaatca gattaacctt
     2461 ccataatccg gtgaaattca gagggaaaat ttccgctgcc caatcagaca agaaagactg
     2521 aagaag
//
LOCUS       DAOQGJ010000404         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26265_length_2526_cov_2.443140, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000404 DAOQGJ010000000
VERSION     DAOQGJ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26265_length_2526_cov_2.443140"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PK683_07485"
     CDS             complement(<1..849)
                     /locus_tag="PK683_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNH08327.1"
                     /translation="MKLHHSKIWFASAVFLAMFTVWNFSFMSFLKSFSVLIHEICHAS
                     VILISGGGLEKIHLTPFESGEVLGKISGWSYLPAVSAGYIGSSLFGGYLLQKGFSTES
                     ARIQTAFISFILSFFTLFYTDTLSYAQITGIIWSLLLFVCIFIPEKIHSMFLIFLGVS
                     CAMYSIYDLTDFTGNIELTDAGLLASGSRQMLRSTGLDISLKKLSYIIAGSWSLISIF
                     TVVFFLKKTFIRNFTEGEYGLEEMLAEYSKGRVATDTAEWFLKKGIDLNGKPLPEKIL
                     KEIRSKE"
     gene            complement(846..1301)
                     /locus_tag="PK683_07490"
     CDS             complement(846..1301)
                     /locus_tag="PK683_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08328.1"
                     /translation="MKTLIAFFLIMVNLHSTDEKTEYPFFEFQNQSTVILKNAKIQIC
                     LVESENLLNREKETVEVCLPAAENLDSVWQYLRKAKITKKTTVWRFYEESVKTNPMLV
                     NSQTEYFLQSIILKKKDRKKIHLELLNDRGGYYYIAANPDKIIFEKEEK"
     gene            complement(1298..2278)
                     /locus_tag="PK683_07495"
     CDS             complement(1298..2278)
                     /locus_tag="PK683_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08329.1"
                     /translation="MKILFFFIILYSSISAGDGIFYYEEALKAEKISPYFAIPLYETF
                     LEKSEVGSFRKAAISRLFDLYMQHGKYEDLIILSSKLPLDKKRQKKLQQIFRHIAKDT
                     DLPEEDIEGFFKDMINGSLGSTKETLNRHMKGKNQLMVNYIFALKFRTKDFDSLEYLI
                     REYPEINPILRLAYFVNKEGNEAKEYIRETASLTELSDEQKMELLYLYGIYFMKRKNY
                     ANSARFFMMSASYDSNEDGRLSGGILSTSKALYLTGRKNEACALISGKKLNIRNESDE
                     FMRMFCSIEESKKLPKLIGSFSILAGRESGQVFELYIGHNARRKQKVLKK"
     gene            complement(2275..>2526)
                     /locus_tag="PK683_07500"
     CDS             complement(2275..>2526)
                     /locus_tag="PK683_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08330.1"
                     /translation="TFAAPLWGKIGQELSKRKLVPEEKFQFSRKAQWAAVCRESGLPA
                     GDDCTDKRQDLFTKNSKPASVCKFHSRTDDKELLKKLFQ"
BASE COUNT          689 a          540 c          482 g          815 t
ORIGIN      
        1 ctcctttgac ctgatttcct tcagtatttt ttcagggagc ggttttccat tcagatcaat
       61 tccttttttc agaaaccact ctgccgtgtc tgttgcgact ctgccttttg aatactcagc
      121 cagcatttct tcaagcccat actctccttc tgtaaaattt cttatgaaag tttttttcag
      181 aaaaaagaca actgtaaata tggaaatcag actccagctg cctgcaatga tgtaactgag
      241 ttttttgagt gaaatgtcca gccccgtcga tctaagcatc tgtcggcttc ctgaagccag
      301 aagtccggca tccgtcagct ctatattgcc ggtaaagtcc gtcagatcgt agatgctgta
      361 cattgcacac gagactccga gaaaaataag aaacatagaa tgtatttttt cgggaataaa
      421 aatgcaaaca aaaaggagaa gtgaccagat aattccagta atctgtgcat aggaaagtgt
      481 atcagtataa aataatgtaa aaaatgacag aataaaagaa ataaatgcag tctgtatcct
      541 tgcagattct gtgctgaatc ctttctggag aagatatcct ccgaaaagag atgagccgat
      601 gtatccggca gaaactgcag gtaagtaact ccatccggaa atctttccta ggacttcacc
      661 tgactcaaaa ggagtaagat ggattttttc cagtcctcct cctgaaatca atatgacgga
      721 agcatgacag atttcgtgaa tcagtactga aaaactcttc aggaacgaca taaaactgaa
      781 attccataca gtaaacatcg caagaaatac tgctgatgca aaccagattt tactgtggtg
      841 gagtttcatt tctcttcttt ttcaaatatg attttatccg gatttgccgc tatatagtaa
      901 tagccacccc tgtcgttcag aagttccaga tgaattttct ttctgtcctt tttcttcaga
      961 atgatagact gcagaaaata ttccgtttga gaattgacaa gcataggatt ggtctttacg
     1021 ctctcttcgt aaaatctcca gactgttgtt ttctttgtga tcttggcctt gcgaagatac
     1081 tgccatacag aatccagatt ttccgctgcg ggcagacaga cttcgacagt ttctttttcc
     1141 ctgttcagaa ggttttctga ttcaacaagg cagatttgga tttttgcatt tttcagaatg
     1201 accgtagact ggttctgaaa ttcaaaaaaa gggtattctg tcttctcgtc tgtactgtgg
     1261 agattcacca taatgagaaa aaaggcaatc aatgtcttca ttttttcagt accttctgtt
     1321 ttcgtcttgc attatgacct atataaagct caaatacctg acctgactct cttcctgcca
     1381 gtatggaaaa tgagccgatg agtttcggaa gttttttgga ctcctcaatt gagcagaaca
     1441 tccgcatgaa ttcatcagac tcatttctga tattgagttt ttttcctgag atcagtgcac
     1501 atgcttcgtt ttttcttcct gtcagataaa gagcctttga agtgctcaga attccgcctg
     1561 aaagtctccc gtcttcattt gagtcgtacg atgcgctcat catgaagaac ctagcactgt
     1621 ttgcataatt ttttctcttc ataaaatata tgccgtatag ataaaggagc tccattttct
     1681 gttcgtcgct gagctcagtc agagaagccg tctccctgat atattccttt gcctcgtttc
     1741 cttccttgtt cacaaagtag gcaagacgga gtatcgggtt aatttcaggg tattcgcgga
     1801 tcagatattc cagtgaatca aagtccttag tcctgaattt gagagcaaaa atataattca
     1861 ccatcagctg atttttccct ttcatatgac ggttcagagt ctctttcgtg cttcccagac
     1921 ttccgtttat catatccttg aaaaaccctt ctatatcctc ttcagggaga tctgtatcct
     1981 ttgcaatatg tctgaaaatc tgctgcagtt ttttctgtct ttttttatcc agtgggagtt
     2041 ttgatgacag tatgatcaga tcctcatact tcccatgctg catatacagg tcgaacagtc
     2101 tggatatggc tgcctttctg aatgagccta cttccgattt ttccagaaat gtctcataaa
     2161 gaggaatcgc aaaatagggt gatatttttt ccgccttcag ggcttcctcg tagtaaaata
     2221 tcccgtctcc tgccgaaatg gacgaatata atataatgaa gaaaaataaa attttcattg
     2281 gaaaagtttc tttaaaagtt ctttgtcatc cgttctgctg tgaaacttgc acactgatgc
     2341 cggtttggag ttctttgtaa acagatcctg ccttttgtca gtgcagtcgt ctcctgcggg
     2401 aagaccggat tccctgcaga ccgcagccca ttgtgccttc cgggaaaact gaaatttttc
     2461 ttcagggaca agttttcttt tagaaagctc ctgaccgatt tttccccata gaggcgctgc
     2521 aaatgt
//
LOCUS       DAOQGJ010000405         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26317_length_2523_cov_2.383306, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000405 DAOQGJ010000000
VERSION     DAOQGJ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26317_length_2523_cov_2.383306"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..342)
                     /locus_tag="PK683_07505"
     CDS             complement(82..342)
                     /locus_tag="PK683_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08331.1"
                     /translation="MILSLSFFLKGCYTEFLELPDQYAEISGKCAREKRKRRLCTLAL
                     FGSCVEDMKSKTAVSVSPGAVCAEMIFFIDGLCDRRSLECTP"
     gene            544..1272
                     /locus_tag="PK683_07510"
     CDS             544..1272
                     /locus_tag="PK683_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNH08332.1"
                     /translation="MSNIKTCFIPAAGFGKRMKELTVDLPKPLLKVDGFTLLKTAVSR
                     AKLFAAERFVINAHYHSEKIAQEISLYRNTETSVSEEKGEILGTAGGIRTAVHRGFIK
                     ENEKIMIVNPDMLYFPDHSEWKPEADSESVCTLFLGKKNPEDSFTGFAFRDERNIHTH
                     PDGQFFYIGICILDVSVLNYLEPDKYADLSEDFKRLAVQGKLFGKIFPGKFYDTGEKE
                     KYKYLKNAGLVRQDNELVNMLESF"
     gene            complement(1281..1589)
                     /locus_tag="PK683_07515"
     CDS             complement(1281..1589)
                     /locus_tag="PK683_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08333.1"
                     /translation="MIENSLDQTETRRIEDFINSLNADQLSYLNRMIIERFRVLEKEK
                     IEKDLQKFRLGEKVAFQDNEGIERKGVVIRINKKSISVHTDDRTDWKVHPRLLRKFHG
                     "
     gene            complement(1591..2343)
                     /locus_tag="PK683_07520"
     CDS             complement(1591..2343)
                     /locus_tag="PK683_07520"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788940.1"
                     /note="involved in tryptophan biosynthesis; amino acid
                     biosynthesis; converts
                     1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to
                     C(1)-(3-indolyl)-glycerol 3-phosphat; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HNH08334.1"
                     /translation="MSIPPILQKIVQEKYSEIAQIPEYSEFSRKTYSLKQSILGKKTA
                     IIAECKKGSPSAGILKADYFSKKIALEYERFGAAGISVLTDRKFFYGSISDLEDVSSS
                     VGIPVLRKDFIIDELQIREARYFGASAFLLIARILEKKKLRSLLEFGKKIGLECLVEI
                     HTKEEAADALDSGAEIIGINSRDLDTFQINNSLIPEIAEYLPDYIVRVGESGVHGKED
                     LLKMGKRINAYLIGTYFMESENLSESFHALMN"
     gene            complement(2345..>2523)
                     /locus_tag="PK683_07525"
     CDS             complement(2345..>2523)
                     /locus_tag="PK683_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH08335.1"
                     /translation="MSSSGFRACISTLRKLNAKEGSLKMSNIRPAVKRIFDVIELTTL
                     FDIYATEDEALAAF"
BASE COUNT          775 a          512 c          460 g          776 t
ORIGIN      
        1 ctgaatgact gtattattca tagtgctatt tttttacgaa tgactgacca ttcaagagaa
       61 tttctttaca tggattcgga tttaaggagt gcattccagc gatcttctgt cacaaagccc
      121 atcaatgaaa aaaatcattt cagcacagac tgctcccgga gatacactca cggccgtttt
      181 tgatttcata tcttccacac aggaaccgaa tagagccaaa gtgcaaagtc ttctttttct
      241 tttttccctg gcacattttc cggagatttc cgcatattgg tctggaagtt caagaaactc
      301 agtgtagcac cctttcaaaa aaaaagataa agacagaatc aatatgacgc accgatttcg
      361 gcttttggaa cacatttttt tatggttgca tttgtaaatc ggattgtcaa ttgattttaa
      421 taaagatata ctgattacca aaagtaattg cgctttttac attcgaaaac cttgttttac
      481 aggctcacta aagtagaaat acgcaaagac ttttgaatat aagtatatgt gcaggagccg
      541 ccaatgtcca atataaaaac atgttttatt cctgcagcag gattcgggaa aagaatgaaa
      601 gaactcacag ttgatctgcc gaaacctctt ctgaaagtcg acggattcac tcttctgaaa
      661 acagccgttt cgcgggcaaa acttttcgcc gcagaaagat ttgtcatcaa tgcacattat
      721 cattcagaga aaatcgctca ggaaatctcg ctttaccgaa atacggaaac ttcagtatca
      781 gaggaaaaag gagaaatcct gggaactgca ggaggaatca gaactgcagt ccacagaggc
      841 ttcattaagg aaaatgaaaa aataatgatc gtaaatccgg atatgcttta ttttcccgat
      901 cattctgaat ggaaaccgga agcagattcg gaatctgtat gcacgctgtt tttaggaaaa
      961 aaaaatccgg aagactcatt tacaggtttt gccttcagag atgagagaaa cattcacact
     1021 catcccgatg gacagttttt ttatatagga atctgcattc tggatgtttc cgttctgaat
     1081 tatctggagc cggataaata cgcagattta tcagaggatt tcaaaaggct tgctgtgcag
     1141 ggaaaactct ttggaaaaat atttcccgga aaattttacg atacaggaga aaaggaaaag
     1201 tataaatatc tgaaaaatgc aggtctggtc agacaggaca acgaacttgt gaatatgctt
     1261 gagagttttt aagcctctcg tcagccatga aatttcctca aaagacgggg atggactttc
     1321 cagtctgtgc ggtcatcagt atgtacactt attgattttt tatttattcg aatgacaacg
     1381 ccctttcttt caatgccctc attgtcctgg aacgccacct tctcgccaag tctgaacttc
     1441 tgtaaatctt tttcaatttt ttctttttca agaacacgaa accgttcaat aatcatccgg
     1501 tttaaataac tgagctgatc cgcattcaaa gaatttataa aatcttcaat tcttctcgtc
     1561 tctgtctgat ccagcgaatt ttcgatcatc ttagttcatc agtgcatgaa agctttctga
     1621 aagattttca gattccataa aatacgttcc aatcaggtag gcattgattc tctttcccat
     1681 ttttaaaaga tcctcttttc catgaacacc cgactccccc actctgacta tataatcagg
     1741 aagatactcc gctatttccg gaatcagtga attgttgatc tgaaatgtat ccagatctct
     1801 ggaattgatg cctatgattt cggctcctga atccagtgca tctgctgcct cttcttttgt
     1861 atgaatctcc accagacatt ccagtccgat tttttttcca aattcaagaa gacttctgag
     1921 tttctttttc tccagaatcc tcgcaatcaa aaggaaagcg gatgcaccaa aataacgggc
     1981 ctcccgaatc tgaagttcat caatgataaa atccttcctg agtacaggaa ttcctactga
     2041 ggaggaaaca tcttccaaat cagaaattga accgtaaaaa aattttctgt cggtcagaac
     2101 agatatgcct gctgcaccga atctttcata ttccagagca attttcttag aaaagtagtc
     2161 tgctttaaga atgcctgcgg aaggacttcc ttttttgcat tctgcaataa tggcagtctt
     2221 tttccctaga atagattgtt ttaatgaata ggtttttctg gagaattcag aatactccgg
     2281 aatctgagcg atttcggagt atttttcttg gacgattttc tgaagaatcg gcggaataga
     2341 caaattaaaa tgccgctaaa gcctcatctt ctgttgcata gatatcaaac agagtagtca
     2401 gttctatgac atcaaatatc ctttttacag ccggacggat attggacatt ttaagagaac
     2461 cttcttttgc attcaatttt cttaaagtac tgatacatgc tctgaatcct gaagaagaca
     2521 tat
//
LOCUS       DAOQGJ010000406         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26399_length_2518_cov_2.594397, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000406 DAOQGJ010000000
VERSION     DAOQGJ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26399_length_2518_cov_2.594397"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PK683_07530"
     CDS             complement(<1..1315)
                     /locus_tag="PK683_07530"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH08336.1"
                     /translation="MLSAIQNEYHTGEETVRRRIYHILIREYGIVLKRELRKSPAALN
                     FILKEGITYSQFLNKQLEETLESEGFVPEGSSFFEEMYSEIPSAESFDDVTAKNLLLQ
                     FRARFENILSGTDPSLDEKLTKGMDRLLDLLLNSPDELKRFNLQERNFVNKLLETLRK
                     NYLGDSAFLISASLLIERQSLQISNLANERIITKMTELLGSERKLKDEILLNHRQVEE
                     EIRHAEKIQRHLLPKSFPLNSRLRFHSRYRPMAGVGGDFYDISSLLCNDELSSGKKVQ
                     KTAFLIADVSGHGIPAAFIASMTKISWKNAIEKLVLPGEILKRMNSDLLENTAGNFIT
                     ALAGVFDTSSDADQTVFRMSSAGHIPPYIIRKNGKAESIEMKGRLLGVFRDVPLEEKE
                     IILEKGDRIFFYTDGLPEARNRTSGNLLDSERLYSIFNESKDLDGE"
     gene            1547..1771
                     /locus_tag="PK683_07535"
     CDS             1547..1771
                     /locus_tag="PK683_07535"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNH08337.1"
                     /translation="MSAKEDSMATNLALDDSLIEEARIAGRHRTKKDAVTMALREYIQ
                     KRRQLEILDLLGTLDFSEKLLDYKKRRLRK"
     gene            1768..2193
                     /locus_tag="PK683_07540"
     CDS             1768..2193
                     /locus_tag="PK683_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNH08338.1"
                     /translation="MILVDTSVWSQVLRRNSGRLNETQKKIREKFSELVMEGSIVMMG
                     VIRQELLSGIRNGSDFENLKKKLIFFPDTEILQEDYEKAAEFFNLCRAKGISGSSIDF
                     LICAVAVRCRLTVFTLDQDFKNYAKILPLQILNIDESNS"
     gene            2284..>2518
                     /locus_tag="PK683_07545"
     CDS             2284..>2518
                     /locus_tag="PK683_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08339.1"
                     /translation="MKFKSILVLLFAAVLAVSNCNNKKDTSARDSLLFTFLAGMAGSG
                     SGAGSCELNSKSMCIYTVGPMPAGFNFASNCTNI"
BASE COUNT          726 a          529 c          529 g          734 t
ORIGIN      
        1 cctctccgtc caaatctttc gattcattga atatggaata cagtctttct gagtcaagca
       61 ggtttcctga agttctgttc ctggcttccg gcagaccgtc agtataaaag aaaatcctgt
      121 cccctttttc cagaataatt tccttttctt ccagtggcac atcccggaaa actcccagaa
      181 gccttccttt catttcaatc gattctgcct ttccgttttt tctgataata taaggaggta
      241 tatgaccggc actcgacatc cgaaagactg tctgatccgc gtctgatgaa gtatcaaata
      301 cgccggcaag tgctgtaatg aaattgcctg ccgtattttc aagcagatcg ctgttcattc
      361 ttttcagaat ttcaccggga agaacgagtt tttcaatcgc atttttccat gatatctttg
      421 tcatggatgc aatgaatgca gccggaatgc cgtgaccgga aacgtctgca atcaggaagg
      481 ctgtcttctg cacttttttt ccggaggaca gctcgtcatt acacagtaat gacgatatat
      541 cataaaaatc ccctcctact cctgccatag gtctgtacct ggaatgaaat ctgagccttg
      601 aattgagagg aaatgacttg ggaagaagat gcctctggat tttctcagca tgtctgatct
      661 cctcttccac ctgtctgtga ttcagaagaa tctcgtcctt cagttttctt tccgatccca
      721 gaagctcagt catctttgtt atgattcttt catttgccag attggaaatt tggagtgact
      781 gacgttcaat caagagcgac gcagaaatca gaaatgcact gtctccaaga taattctttc
      841 tgagagtttc gagaagcttg tttacgaaat ttctttcctg aaggttgaat cttttcagct
      901 catcaggaga gttcagaagc agatccagaa gcctgtccat tcctttggtc agtttttcgt
      961 caagagacgg atcagtgccg gaaagaatat tttcaaatct tgcccgaaac tgaagaagga
     1021 gattctttgc agtgacgtca tcaaaacttt cagcagaagg aatctctgaa tacatctctt
     1081 caaagaatga actcccttcc ggaacgaatc cttctgattc aagtgtttct tccagctgtt
     1141 tgttcagaaa ctgtgaatat gtgattccct ctttcagtat aaaattcagt gcagcagggc
     1201 ttttcctcag ctcccttttc agaacaatgc cgtattcacg gatcagtata tgatatattc
     1261 ttcttctgac tgtctcctca ccggtatggt actcattctg tattgccgag agcatatatc
     1321 ttattcttgc tgtccctgtt ccagatttcc attcagttca gaataatcaa ccagaatact
     1381 gaatgctgag acagcctgcc tgcaaagatt ttcaggcaga cagctgcatt tttcgcggca
     1441 aaatttccga tgatcccgcc tattaaaaaa agactttaca ctttttttca cacacgagta
     1501 agtgtagaaa aaagtgtagc agttataggt gtctgcagca gcttcaatgt ctgcgaagga
     1561 ggacagtatg gcgacaaacc ttgcactcga cgacagtctc atagaggagg ccagaattgc
     1621 aggaagacac aggaccaaaa aggatgcagt gacaatggca ctcagggaat acattcagaa
     1681 aagaaggcag ctggaaattc tggatctttt gggaacactg gatttcagtg aaaaacttct
     1741 ggactataaa aaaagaagac tgagaaaatg atactcgtcg atacgtcggt ctggtcgcag
     1801 gttctcagaa gaaattcagg aagactgaat gaaactcaga aaaaaatcag ggaaaaattc
     1861 agcgaacttg taatggaagg aagcatcgta atgatgggag tcattcgtca ggagcttttg
     1921 agcggcattc gaaacggttc tgacttcgaa aatctgaaaa aaaaactgat ctttttcccc
     1981 gacacggaaa tacttcagga agactatgaa aaggctgcag agtttttcaa tctatgcagg
     2041 gcaaagggaa tcagcggaag cagtatagat tttctcatct gtgcagtggc agtccggtgc
     2101 aggctgaccg tattcaccct ggatcaggat ttcaaaaatt acgcaaaaat ccttcctctg
     2161 cagattttaa atattgatga gagtaattct taaaatatga taaaaaaaac tgaagctttt
     2221 ttgcggattt ttcgtcttat caaataagca aagattttcg gaaaataatc acgaggtgtg
     2281 aatatgaaat ttaaatcaat actggtactg ctgtttgcgg cagttttggc tgtatccaat
     2341 tgcaacaaca aaaaagatac ttccgcaagg gattctcttt tatttacatt tctggcaggg
     2401 atggccggtt caggttctgg ggcaggaagc tgcgaactga attcaaagtc tatgtgtatt
     2461 tacactgtcg gaccgatgcc tgctgggttc aattttgcat ccaactgcac aaacattg
//
LOCUS       DAOQGJ010000407         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26455_length_2515_cov_3.023984, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000407 DAOQGJ010000000
VERSION     DAOQGJ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26455_length_2515_cov_3.023984"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PK683_07550"
     CDS             complement(<1..320)
                     /locus_tag="PK683_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08340.1"
                     /translation="MISIASCGISVSLSQLFLGIAFIFHMSDRDRKKIHPSLLLFSGF
                     LLFLSFFISFFANHSFSEYRLITKSECKDFLLFSGFILTSGLERTERETVYKGFKLLL
                     FLLIL"
     gene            376..1647
                     /locus_tag="PK683_07555"
     CDS             376..1647
                     /locus_tag="PK683_07555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH08341.1"
                     /translation="MRVIYFTDTFLPKIDGITISIKNFCEHLSARGYEFLICCPEYGE
                     GDFERMGDRIHVTRFMSAYLPSYKDIKVVLPSPNRISKIIEDFKPEVCHIHTPGLLGQ
                     YAISACEKYAIPSVGTYHTLMAEQNTYVSLYRILKLDKLFLKINKFNKALTLKNLLKF
                     QKFENFNIQKKIILKLCNNLYERCDIVVSPSHLLKAQLEEYEIDRPIRVISNGMDLSR
                     FKGSVKKCSDAPKILHVGRISYEKNCDVVINAFKIIHAKHPGTTLTIIGDGPALPSLK
                     LQAEQLGLKDKVIFKGFVPNSELQNVYPEYDLFMSASTMETQGLVILEAIACGLPAVG
                     VKSFAIPELVQEGRNGFNAEPFQPEEMAALAMKILENAELYKTFSENSLSISREHEIS
                     KCVDKMDALYQEMKSFKNKKKKHSFLNSLLA"
     gene            complement(1621..>2515)
                     /locus_tag="PK683_07560"
     CDS             complement(1621..>2515)
                     /locus_tag="PK683_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNH08342.1"
                     /translation="KGIWGLWKMYKEISKLGPFHSVIDLHGSLRSILFSFIFRMKKVP
                     VFKINKGRKEKNAQIRQKNKILTPLPHTVERYLKVFEHAGFPTELREGPWINVDPYSK
                     ILASEFLRSQNLQRGDSLWIGFAPFAGHELKEWPIAKSKDLVSLILKEFKAKVFLFGS
                     HEELSRLQEIKSENEECILVAGTKLGIKGDLGVLEKMDLLVGMDSSNLHIMALLGKPV
                     IGLYGTTHPFSGFGPFMQEKEGVLQVDLACRPCSIYGNTECYRKDFACMELIHPADVI
                     RRIRERLSDKIRKLSEKRIQK"
BASE COUNT          766 a          546 c          500 g          703 t
ORIGIN      
        1 agtataagaa gaaacagaag aagcttgaag cctttataaa ctgtctctct ttcggttctt
       61 tcaagtcccg aagtcagaat gaacccgctg aaaagcagaa aatccttgca ctcggatttt
      121 gtgatgagtc tgtattcgga aaatgaatgg tttgcaaaaa aggaaatgaa gaagctcaga
      181 aaaagaagaa agcctgaaaa aagaagaagg ctcggatgga ttttctttct gtctctgtct
      241 gacatatgga aaataaaggc gattccgagg aaaagctgac tcagtgatac tgagattccg
      301 caagatgcta ttgaaatcat gagactgagt aaaaaaaact tttgcaattt cctcatccta
      361 ttttatcctt gtgacatgag agtcatatat tttaccgata cctttctacc aaaaatagac
      421 ggcattacta tttcaatcaa aaacttctgt gaacatcttt ctgcaagagg ttatgaattc
      481 ctgatctgct gtcctgaata cggtgaagga gattttgaga gaatgggaga cagaatccat
      541 gtcacccgtt ttatgagtgc ctatctccct tcttacaagg acataaaagt agtccttccc
      601 agtccgaaca ggatttccaa aattatagaa gatttcaagc ctgaggtctg tcatatacat
      661 actccagggc tgctaggcca gtatgcaatc agtgcctgcg aaaaatatgc gattccaagt
      721 gtaggtacat accataccct gatggcagaa cagaacactt acgtatcttt gtacaggatt
      781 ctcaaactcg acaaactttt tctgaagatc aataaattca ataaagcact gactctcaaa
      841 aatcttctga aatttcagaa atttgagaac ttcaatattc agaaaaagat catactgaaa
      901 ctctgcaaca atctgtatga aagatgtgat attgtagtct ccccttccca tctgctcaag
      961 gctcagcttg aggaatacga aattgacaga ccgatcagag tcatttcaaa cggaatggac
     1021 ctttccagat tcaagggctc agtaaaaaaa tgctcagatg ctccaaaaat ccttcatgtg
     1081 ggcaggatct cctatgaaaa aaactgtgat gtcgtcatca atgcattcaa aatcattcat
     1141 gcaaaacatc caggaacaac tctgaccatc atcggcgacg gccctgcact tccttcactg
     1201 aaacttcagg cagaacagct tggactgaag gacaaagtca ttttcaaagg atttgtaccg
     1261 aactctgaac ttcagaatgt atatcctgaa tacgatctgt ttatgtctgc atcgactatg
     1321 gagactcagg gacttgtgat tctagaagca attgcctgcg gacttcctgc agtgggagtc
     1381 aaatccttcg ccataccgga acttgttcag gaagggagaa acggcttcaa tgcagaacct
     1441 ttccagccgg aagagatggc ggctctggca atgaaaatac tggagaatgc tgaactttat
     1501 aaaacgttct cggaaaactc tctcagtatt tcgagagagc atgagatttc gaaatgcgtg
     1561 gataaaatgg atgcccttta ccaggaaatg aaaagcttca aaaacaaaaa aaagaagcat
     1621 tcatttttga attctcttct cgcttaactt tctgatctta tcagaaagcc tttccctgat
     1681 ccggcggatg acatctgccg gatgtatgag ttccatacag gcaaagtcct ttcggtagca
     1741 ttcggtattt ccgtatatac tgcagggtct gcatgccaga tccacctgca gaacaccttc
     1801 cttttcctgc ataaatggac cgaagcctga aaatggatga gttgtcccat acaggccgat
     1861 cacaggtttt cccagaagtg ccataatgtg caggttggaa gaatccatcc ctacgagcag
     1921 atccattttt tccagaactc ccagatctcc cttgatcccc agttttgttc ctgctacaag
     1981 aatgcattct tcattttccg atttgatttc ctgaagacgg gaaagttctt catgtgaacc
     2041 gaacaagaac acctttgcct tgaactcctt cagaatcaaa gacactaaat cctttgattt
     2101 tgcgatcggc cattccttca gttcgtgtcc ggcaaaagga gcaaatccta tccacagaga
     2161 atctcctctc tgaagattct gtgaccttaa gaattcactt gcaagtattt tggaataagg
     2221 atctacattg atccaggggc cttcccgaag ttctgtagga aaacctgcat gttcgaatac
     2281 tttcagatac ctctccactg tgtggggcag aggtgtgagt attttattct tctgtctgat
     2341 ctgagcgttt ttttcctttc tgcccttgtt gattttgaag acaggaactt ttttcattcg
     2401 gaaaatgaag gaaaacagga tacttctcag actcccgtgc agatcaatga cactgtgaaa
     2461 aggtccaagc ttggaaattt ccttgtacat tttccaaagt ccccagattc ccttg
//
LOCUS       DAOQGJ010000408         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26508_length_2511_cov_3.673860, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000408 DAOQGJ010000000
VERSION     DAOQGJ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26508_length_2511_cov_3.673860"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PK683_07565"
     CDS             complement(<1..592)
                     /locus_tag="PK683_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH08343.1"
                     /translation="MKIFIIAAALMHLTECIHIPKTTNHNPDLKNMEIEDYKFHIRTS
                     GNKDKKPIVVIHGGPGGDFQYLLPLEKLADEFHILFYDQRMTGLSQRESEKKPGIEQD
                     IEDLDRIIKTFSAHQKVILIGHSYGGMLAAGYTARHSESVASAVIIEPGILNRESAFE
                     FIERFKSRTSIFSLFSIVPPLLKSIFVKTEDGHERYD"
     gene            913..1242
                     /locus_tag="PK683_07570"
     CDS             913..1242
                     /locus_tag="PK683_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08344.1"
                     /translation="MNKSWLIFIALLLFGGCASSAPKDSLEGFKNDLRSLKIRTVPVT
                     KECSLPDSESVKLIKESAARFKSDNPEHTPAFAEQYSAGFICGYYNVHPSCEKTCVEG
                     LMKQMAK"
     gene            complement(1244..>2511)
                     /locus_tag="PK683_07575"
     CDS             complement(1244..>2511)
                     /locus_tag="PK683_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH08345.1"
                     /translation="GRIQYNYSLAGNGAGLLGGLEGDYFSYKGDKEHWSNAELSDAAN
                     GFPPTLNGEIRKLGPWFEWIKSKPIRKRAVFAQLVSGKILNNFMEITLGIRNDFTSQN
                     FRGIDRPFSDTNRTLDYFGISQRFSAADKPAMTDPVLSSLIPNEKRTFSSTNPRLGFV
                     FFPTKTLTVKAMAGTAFREPAPSEQFGANTFSLASNPRGLKPEKIRTYELGADWFITK
                     SLNLRVNGFHTLFENMITYAASGNALANVYSLTTRGVEAELLYAFSRNVKGFVNYSRN
                     WRIDERILDKTVSIDRHSVTWVPASNANFGISGTYEKIQYAFSVQHQGKVKRRTSDYG
                     PIDPYTGYPSAVPQLEPAGTFYYRPKSVDAWSNLTLRFGYMFDEMKSVHITVINSLNS
                     RQNLIKNNYYAFDYLREPRRILLEANLAF"
BASE COUNT          679 a          538 c          510 g          784 t
ORIGIN      
        1 aatcataccg ttcatgcccg tcctctgttt ttacgaatat tgattttaac aagggaggga
       61 caatgctgaa taaagaaaaa atcgaagttc tgcttttgaa tctctcaatg aattcaaaag
      121 cagattctct gtttagaatg cctggttcaa taatgactgc actggcaaca gactctgagt
      181 gtcttgccgt atatcctgct gcaagcattc ctccgtatga gtgaccgatc aaaataactt
      241 tctgatgagc ggaaaatgtt tttataattc tgtcgagatc ttcaatatcc tgctcaatgc
      301 ctggtttctt ttcactctct ctttgggaaa gcccggtcat tctctgatca taaaacaata
      361 tatgaaattc atcagcaagt ttttcaagag gaaggagata ctgaaaatct ccgcctggtc
      421 cgccatgaat gactacaatc ggttttttgt ccttatttcc tgacgttctg atatgaaatt
      481 tgtaatcttc aatttccata tttttcaagt caggattatg gtttgtagtt tttggaatat
      541 gtatgcactc tgtcagatgc ataagggccg ctgcaataat aaaaattttc ataaatttct
      601 cctaaaagac ctgtcagggt acaatttgac aaacccgaat cttcggatat ctgccaaaac
      661 ccggaaaagt tttacaattt caatgtcttc ttctcattga ttattcaaga gtataaatga
      721 tgctgaaaaa acttcattat catttgataa cagtgctgaa aaaatatctt tatccagcga
      781 gcttaaagaa tcagcaaagg ggctgcttaa gcccctgagc ggcttccata cttgcgacgc
      841 tctgctgtcg gaaatccggg agacaatact tttcttgaca atttatacaa tttatttatt
      901 catagaatca ctatgaataa aagctggctg atatttattg cgttattact ttttggcgga
      961 tgtgcttcct cggctcccaa agacagtctg gagggtttta aaaatgatct gcgcagtctg
     1021 aagatcagga cagttcctgt aacaaaggaa tgcagtcttc cagacagtga gtctgtaaaa
     1081 ctgatcaagg aatcagccgc aaggttcaag tcggacaatc ctgagcatac tccggctttt
     1141 gctgaacagt attctgcagg gtttatatgc gggtactata atgtgcatcc atcctgcgaa
     1201 aagacctgcg tggaagggct gatgaagcag atggccaaat aattcagaat gcaaggtttg
     1261 cctcaagaag tattcttctc ggttccctca gatagtcgaa agcgtaataa ttatttttaa
     1321 ttaagttctg tcttgagttt aatgaattga tcactgtgat atgaacactt ttcatctcat
     1381 caaacatata tccgaacctc agcgtcagat ttgaccaggc atcgacagac ttgggtctat
     1441 agtaaaaagt gcctgcaggt tccagctgag gcactgcaga gggatagccg gtataaggat
     1501 ctatcggacc atagtcgctc gtccgccttt ttacttttcc ctggtgctga acagaaaatg
     1561 cgtactgaat tttttcataa gttccgctga ttccgaagtt tgcatttgaa gcaggcaccc
     1621 aggttacaga atgccggtcg atggatacag ttttatccag aatcctttca tcgattctcc
     1681 agtttctgga ataatttaca aaccctttaa catttctgct gaatgcatac agaagttctg
     1741 cttccactcc tctcgtggtc agagaataca cattggccag agcgttgccg gacgcagcat
     1801 atgtgatcat attttcaaaa agtgtatgaa atccgttcac tctcaggtta agactctttg
     1861 taatgaacca gtctgcaccc agctcatacg ttctgatttt ttcaggttta agtcctcttg
     1921 gattggatgc aagagagaat gtatttgctc caaactgttc ggatggtgcc ggttctctga
     1981 aagcggttcc ggccatcgcc tttacagtaa gggtcttggt cggaaaaaaa acaaatccaa
     2041 gtctggggtt tgtggaactg aaggttcttt tttcattagg aattaatgat gagagcaccg
     2101 gatctgtcat tgccggcttg tccgcagcac tgaatctctg agaaattccg aaatagtcca
     2161 gggttctgtt tgtatcactg aacggtctgt caatgcctct gaaattctgg cttgtaaagt
     2221 cgtttctgat tccgagagtg atttccataa aattgttcag tatttttcct gataccagct
     2281 gagcaaaaac tgctcttttc cggatcggct tgctctttat ccattcaaac caggggccaa
     2341 gttttctgat ttccccgttc agtgtcggcg gaaatccgtt tgcagcatca ctcagctccg
     2401 cattgctcca gtgctctttg tctcccttgt aactgaagta gtcaccttcc agtcctccca
     2461 gcagtcccgc cccgtttcct gcaagactgt aattatactg tattcttcca a
//
LOCUS       DAOQGJ010000409         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26538_length_2509_cov_2.650367, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000409 DAOQGJ010000000
VERSION     DAOQGJ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26538_length_2509_cov_2.650367"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PK683_07580"
     CDS             <1..1100
                     /locus_tag="PK683_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNH08346.1"
                     /translation="CSHCGSRAGRERQNELSKGEALLLADELIQMNPREVALIGGEAY
                     LHEGFFEIIKKLTDAGIPVTMTTGGQGVTESISKEAVKSGLKNVSVSIDGTEEHHDLI
                     RRKKGSFAAASRALEHLKNAGMLISSNININRINKNDLEYVFGYLKNLGVSSWQIQIT
                     VPLGRASDRPDMILQPWDLIELMPEIYSLKKKAFDDEILIMPGNNLGYFGPEEAVLRS
                     LNLTDRDHWQGCQAGKFVMGIESDGGIKGCPSLQSVPYIGGNIREKTLDDIWKNSPEI
                     SFARNRTVEDLWGFCRTCVFASHCLGGCSFTAHSLFGRLGNNPYCHYRAKTLSKKNIR
                     ERLVLKKAAPGLPFDHGIYEIQEEPFDSDSR"
     gene            complement(1735..2253)
                     /locus_tag="PK683_07585"
     CDS             complement(1735..2253)
                     /locus_tag="PK683_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08347.1"
                     /translation="MRISTVFLLSMAVSGLYGCNDKWQDEVTLNDLADFEKGFIQAVE
                     SDNPEKVYDYLGEVVPFSSPTCNFNLEKKYKFEKEEFIQKKSIFYNYIFRYELVKKIY
                     NDAEPSKVQYSLPEMLKKKPRITARNDTVNGETIRRRLMLYVETPTRGTIFVIDSNCE
                     KKKCYISYLGRY"
BASE COUNT          710 a          567 c          510 g          722 t
ORIGIN      
        1 cctgttcaca ctgcggttcc cgtgccggca gagaaagaca gaatgaactc agcaagggtg
       61 aagctctttt actcgccgat gagctcattc agatgaatcc ccgtgaggtc gcactgatcg
      121 gaggagaagc ctatcttcac gaaggttttt tcgaaatcat aaaaaagctt acagatgcag
      181 gaattccggt gacaatgact acgggaggtc agggagtcac tgaatccatt tccaaagaag
      241 cagtaaaaag cggattgaaa aatgtatccg tcagcatcga cggaacagaa gagcaccatg
      301 atctgatcag acggaagaaa ggaagttttg ctgctgcttc cagagcacta gagcatttga
      361 agaatgccgg aatgcttatt tcctcaaata ttaacatcaa ccgtattaat aaaaatgatc
      421 tagagtatgt tttcggatat ttaaaaaatc tgggagtatc gagctggcag atacagatta
      481 cagttcctct cggcagggca tccgaccgtc ctgatatgat tctgcagccc tgggatctga
      541 tcgaactgat gcctgagata tacagtctga agaaaaaagc atttgatgat gaaattttaa
      601 ttatgcctgg aaataatctg ggatacttcg gaccggaaga ggcagtgctc agatcactga
      661 atctcacaga cagagaccat tggcagggat gtcaggctgg aaaatttgta atggggattg
      721 aatcggacgg cggaatcaaa gggtgccctt ctctgcagtc cgttccttac ataggcggaa
      781 acatcaggga aaagacattg gacgatatat ggaagaactc accggaaatt tcttttgcaa
      841 gaaacaggac agtggaagat ctctggggat tctgcagaac ctgcgttttc gcatcacact
      901 gtcttggagg atgcagtttt actgctcatt ctctgttcgg cagacttgga aataatccct
      961 actgtcatta tcgtgcaaaa acactttcga aaaaaaatat cagagaaaga cttgtattga
     1021 aaaaagctgc acccggtctg ccgttcgatc acgggatata tgaaatacag gaagaacctt
     1081 ttgattcaga cagccgttga ccttctaaat tcggattgac ctagttccct ttcagcacac
     1141 acaatttatg ccgatgtttt cccgtattct ttgggacttg cataacccaa agaggaatgc
     1201 atccgtcttc cattgtaaaa cagatcaagt tgcagctcag gcttgcccca tccctggaaa
     1261 gttcagcctg tgaccaccta gcggtgaatc cccgcactgc tgcaggcctc ccttcgatcg
     1321 ccccctttgg gagaatgcca ctgagccgct cattccttcg tttccgtcca gccgttcttg
     1381 ggatctgcgg ttttgcaagg acaattctgt catggtgagt gaattttatt gtgtatgcaa
     1441 taaaattagt atcgaaccat ggctatagag aaatttacaa agactaacgt ttttagatct
     1501 tctcttttta taaaatgttt tactttatca atatatctct tttccagttt ctttcttttt
     1561 ttcttatgga agaaaaaaga aagtggagca aagaaagaaa aaaagaaccc gctactcgcc
     1621 gtacaggcac gtatccactc ctgcacgtct tcgcaggctc cctgtgggag atgctttatt
     1681 gtaccaaaca gtgccatcaa acgtaaaatc cttgccgctg tatgccgttt acattcagta
     1741 tctgcctaag tagctaatgt agcatttctt tttttcacag tttgaatcaa tgacaaagat
     1801 tgttccacga gtcggtgttt caacgtacag catcagtctc cttcttattg tttctccatt
     1861 taccgtatca tttctggctg taatcctagg tttttttttc agcatctccg gtaaagaata
     1921 ttgaaccttg cttggttctg catcattata aatcttttta acaagttcat atctgaatat
     1981 ataattataa aaaatagatt ttttctgaat aaattcctcc ttctcaaact tgtatttttt
     2041 ttcaagatta aaattacagg ttggacttga aaaaggaaca acttctccca ggtaatcata
     2101 cactttctca ggattgtccg attcaacggc ctgaataaaa cctttttcaa aatccgctaa
     2161 atcgttcagc gtcacttcgt cctgccattt atcattgcag ccatacagtc ctgatacagc
     2221 catactcagt aaaaatactg ttgatattct caaagtcatt tgcctccctt tttcttcgtc
     2281 tcattataaa tctttagagc cttttctctg ggttcactga aagtatagtg tgcatgcagt
     2341 cctcctgcac cagagccctt gtatcctccg gcctgcagat acccaatctc gcccgatgca
     2401 ccgatgaaag tatgggcagg caattctctg ccgcagacgg tgcccgccta tgcggccacc
     2461 gaaaccttca acaacctgca tgtcaagtat ccgaacttca cctacaagg
//
LOCUS       DAOQGJ010000410         2508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26546_length_2508_cov_3.232776, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000410 DAOQGJ010000000
VERSION     DAOQGJ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26546_length_2508_cov_3.232776"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK683_07590"
     CDS             complement(<1..225)
                     /locus_tag="PK683_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08348.1"
                     /translation="MKKIVLAFSLFILILGSAAVFILANLSGIITPNFITSKIESSMN
                     VRADISSVEINLLSLLSSFEVKGIRLAQRDK"
     gene            complement(371..643)
                     /locus_tag="PK683_07595"
     CDS             complement(371..643)
                     /locus_tag="PK683_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08349.1"
                     /translation="MNSKITLLFLSLFTIAVYSQRGVHPGLNLTGPGGRGSSNWIQPK
                     NKSLEKPNEHSSVPKSSTDPGAKKFECRINEKGQEECTKPKLESNP"
     gene            878..1681
                     /locus_tag="PK683_07600"
     CDS             878..1681
                     /locus_tag="PK683_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNH08350.1"
                     /translation="MQYLIKDLSRMTGIKGFTIRKWQERYRFFQPTLASNGYWYYSND
                     DYVVLSRIVRLLSEGMRISKIASIGRDKILQSRNDKDYSEDERTFVNWMSSQNFAALK
                     SYLDERRSREKFTVFINLTVKNLVVLCGRAWEDGLLTTADEHSFSRWIFGYLRGVVSW
                     MENSAKPVWLVAVFPGDRHEIGALMYYALLRYKKIAAKFVGILPIEHVIRELNRSNQY
                     KAVSFSMTLAQTPAKIDKLRGRIQSRTNIKKVMIGGRGYSLTKHGALKY"
     gene            1683..>2508
                     /locus_tag="PK683_07605"
     CDS             1683..>2508
                     /locus_tag="PK683_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNH08351.1"
                     /translation="MKKKIAVIGTGIAGMGAAYFLRNEFDMEIFEKNDYVGGHTNTVT
                     VQENGKDVNIDTGFIVYNDVTYPNLIRLFSELNVPVKNSQMSFSVQYLPTGLEFCGSG
                     IDGLFAQRKNFLNPSYYRLLYNINRFNNESVSLLDNPEHQDITLAEYIKIYRFHPDLL
                     EYYLVPMSSAVWSTPVDKMMKFPAVTLIRFFKNHGFLGLNTQHQWKTVVNGSKSYMNI
                     LTFSFRDRIRTGKCAIKVSEKKDSVSVEFADGEKRDFDRVILACHADEALAMLESPT"
BASE COUNT          754 a          455 c          539 g          760 t
ORIGIN      
        1 tttatctctt tgagcaagtc tgatgccttt cacttcaaag ctgcttaaga gactcagcag
       61 attgatctcc accgatgaaa tatcggcacg gacattcatg gaagattcaa ttttggaagt
      121 tatgaaattc ggtgtaatga ttcctgaaag attggccaga atgaacacgg ctgccgatcc
      181 gagtatcaga atgaaaagag aaaatgccag aacgattttt ttcatgataa cctcattatt
      241 tatatctgtt cttcagggaa atctgcagat ttcctttgaa atgcagtaca gcctattctt
      301 tctgatggat tagaattttc taatactttt ttcagtttca ctgacagaga gaaaattgag
      361 aatttctgat ttacggattc gattccagtt tcggctttgt acattcctcc tgaccctttt
      421 cattgattct gcactcaaat ttttttgcgc ccggatcagt ggaagatttt ggaactgaac
      481 tgtgctcatt cggtttttcc aaagatttat ttttaggctg aatccagttt gaactccctc
      541 ttccgcccgg acctgtcagg ttcagaccag ggtgaacacc tctttgagaa tagacagcaa
      601 ttgtgaataa ggaaaggaaa agaagtgtaa ttttagaatt catataaatc ctcccgtttt
      661 cgaagaaatg agtctcttct gtatttcggc aatgatttct gcaaaaaagt aaaaagcgag
      721 cattagactg cttgaataat agaaaaaatg tataaaatct attagtttca ctgagatttt
      781 ttttagaatg attataattt tttttgattt tattgaaaag aggtttgaca gttttaaacc
      841 aactttatac atttgccaat agaactttgg gggtaagatg cagtacttaa taaaagactt
      901 aagcaggatg accggaatta aggggtttac tattcgaaaa tggcaggaga ggtacaggtt
      961 ttttcagccg actttggcct cgaacggata ctggtactat tcgaatgacg attatgtagt
     1021 tctttccagg attgtgcgtc ttctttcgga agggatgaga atttcaaaaa ttgcatctat
     1081 cggaagggac aaaattcttc agtccagaaa tgataaagat tattcagaag atgaaaggac
     1141 atttgtcaac tggatgagtt cccagaattt tgccgcactc aagtcatatc tggatgaaag
     1201 gcgttccagg gaaaaattta cagtcttcat caatctgaca gtaaagaatc tggttgtact
     1261 ttgcggaagg gcctgggaag acggtctttt gacaactgcg gatgagcatt cattttccag
     1321 gtggattttc ggatacctga gaggcgttgt ttcatggatg gaaaactctg caaaacctgt
     1381 ctggcttgtg gctgtatttc cgggggacag gcatgagatc ggtgcactga tgtattatgc
     1441 acttctccgt tacaaaaaaa ttgctgcaaa gttcgtggga attcttccta tcgagcatgt
     1501 catcagagaa ctgaacagat cgaatcagta taaggcggtc agtttttcaa tgacccttgc
     1561 tcagacccct gccaaaatcg ataaactcag aggaagaata cagtccagaa caaatatcaa
     1621 aaaggtgatg atcggcggac ggggttattc tctcacaaaa catggagcac tgaaatactg
     1681 atatgaagaa gaaaattgca gttatcggaa caggaattgc aggtatggga gcagcttact
     1741 ttctgcgtaa tgagtttgat atggaaattt ttgagaaaaa tgattatgta ggcggacata
     1801 caaatacagt gactgttcag gagaacggaa aggatgtcaa tattgatacc ggtttcattg
     1861 tttacaatga tgttacctat ccgaatctaa tccgtctttt cagtgaactc aatgtacctg
     1921 tcaaaaattc acagatgtct ttcagtgttc agtatcttcc tactggactg gaattctgcg
     1981 gatctggcat agacggtctt tttgcccaga gaaaaaattt cttaaatccg tcttattata
     2041 ggctgctcta taatataaac agattcaata atgaatctgt aagtctgctt gacaatccag
     2101 agcatcagga tattactctt gcggaataca taaaaatata ccggttccat cctgatctgc
     2161 ttgaatacta tcttgtgccg atgagttcag ctgtctggtc gactccagtc gacaagatga
     2221 tgaaatttcc tgctgtcacc ctgatccgtt tctttaaaaa ccacgggttt ctggggctca
     2281 atactcagca tcagtggaag actgtcgtaa atgggtcaaa atcctatatg aatattctga
     2341 cgttctcctt ccgggacaga atcaggacag gaaaatgtgc aatcaaagta tctgaaaaaa
     2401 aagactctgt ttctgtcgaa tttgcagacg gtgaaaaaag ggattttgac agagtcatcc
     2461 tggcctgtca tgcagatgag gctctggcaa tgcttgaaag cccgacac
//
LOCUS       DAOQGJ010000411         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26569_length_2507_cov_2.438825, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000411 DAOQGJ010000000
VERSION     DAOQGJ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26569_length_2507_cov_2.438825"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PK683_07610"
     CDS             <1..366
                     /locus_tag="PK683_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08352.1"
                     /translation="HKFEKWGNFPLEKENFYSLLRKHSVKNFIIISGDRHIGEYHKIQ
                     EKGLPLMYELTTSPLNKIMEFKVTEPFHPDRIGDFTQKANFGTVRFHETGRKLEISLE
                     LHEIQGETRRIQFSTEDMK"
     gene            complement(369..1049)
                     /locus_tag="PK683_07615"
     CDS             complement(369..1049)
                     /locus_tag="PK683_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08353.1"
                     /translation="MEEAEYKRSRENILKIGFNTLHEIEEKVKAFRIMNQNAAKKRFI
                     ISRENIRDNSNKIILPKAAEIDISATMLLRRKFKAENVFKIFQPDEGIVIISDMTTME
                     GVTLTMDIVTQIMNLGGGAYEGFIDRVDSFTDFINHLKKALFPKMIIIGYIPKEKIQT
                     EIINFVRVKRIDNYLRAMEVTHSVFKPVGYFPRIKQIAITDDPRSWGRFVVDIIREYT
                     KPYLTEQI"
     gene            complement(1176..2198)
                     /locus_tag="PK683_07620"
     CDS             complement(1176..2198)
                     /locus_tag="PK683_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNH08354.1"
                     /translation="MQKKHIYILAGILVAAVAAFFIFSGNSGKKGGKKNDMSTNDQNL
                     YPLGDPGSVFTEATSAIQSSGGALNYNDLMTGLKSGKINFVWELWAMRRNCPENYTGD
                     QCDSAILEYIEKNYNSPEKENLKQLFSKYFKFEKEMKQNEMPKDLSFEERYDLIKKKR
                     KEILGEEDASLVFGMEESQVGFVEATKNFNSLNKKLSGDDRVKKFEDLKKKTYGQYYD
                     AITSREEKFENYQVELNLRESELGGLKGDDKDKKLRSIQEKYFGKEGADRIAKMNEEN
                     LAMEKKIADYEKQEKEFLSQNSSLGDKEKKEKLDALRVKALGQEEADSYASRKEFERL
                     SKEYKQ"
     gene            complement(2255..>2507)
                     /locus_tag="PK683_07625"
     CDS             complement(2255..>2507)
                     /locus_tag="PK683_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNH08355.1"
                     /translation="PIMALTYPITWAGGLYYGLGGDNDGVVPYSSQAWGTWKGGASAY
                     WFATGIDHLQATNLEWSGQTYFDVQGWYLAIANNAKAGL"
BASE COUNT          669 a          556 c          443 g          839 t
ORIGIN      
        1 cataagttcg aaaaatgggg aaattttcct ctggaaaaag agaattttta cagtctcctg
       61 agaaaacatt cagtaaagaa ttttatcatc atcagcggtg acaggcatat tggggaatac
      121 cataagattc aggaaaaagg ccttccgctt atgtatgaac ttacgacaag ccctctgaat
      181 aaaataatgg aattcaaggt gactgaacct tttcatcctg accggatagg agacttcact
      241 cagaaagcaa atttcggaac tgtcagattt catgaaactg gaaggaaact ggaaatcagc
      301 ctggaactgc acgaaatcca aggggaaact cgaagaattc agttcagcac ggaagatatg
      361 aaatagtctc agatctgttc ggtgagataa ggtttagtat attcccgaat aatatccact
      421 acgaaccttc cccagcttct tggatcgtca gtaatcgcaa tctgcttgat cctggggaaa
      481 taacccacag gcttgaacac gctgtgagtg acttccatgg cacggagata gttgtctatt
      541 ctcttgactc ttacgaaatt aataatctca gtctgaatct tttctttcgg aatatatccg
      601 ataatgatca tttttgggaa aagagccttt tttaaatggt ttatgaaatc agtaaaactg
      661 tccactctgt ctatgaatcc ttcgtatgca ccgccgccca gattcataat ctgagtgaca
      721 atatccatag tgagtgtcac gccttccatc gtagtcatat cggaaataat gacaatgcct
      781 tcatccggct ggaaaatttt gaatacgttc tcagccttga actttcttct gaggagcatg
      841 gtagcggaaa tatctatctc tgctgccttt ggaagaatga ttttgttgct gttgtccctg
      901 atattttcac ggctgatgat gaatcttttc ttagctgcat tctgattcat gattcggaac
      961 gctttcactt tctcttcgat ttcatgaagc gtattaaaac caatttttag aatgttttct
     1021 ctacttcgtt tatattctgc ttcttccatc aggaacgttc cataaagctt tgcgaagtca
     1081 tcatacgctt atgtaaatat ttgcctagct ttttttgatt cttttccgca aaaaaacaat
     1141 ttagtgaaaa aaggcctgtt ttacaggcct ttttcttact gcttatattc tttcgacagt
     1201 ctttcaaatt cttttctgga agcataactg tctgcttctt cctgccctaa agcctttact
     1261 ctgagtgcat cgagtttttc ttttttttct ttatcgccga gactgctgtt ctgtgagaga
     1321 aattcctttt cctgtttctc atagtcggca attttctttt ccattgccag attctcttca
     1381 ttcatctttg caattctgtc agcaccttct ttgccgaaat atttttcctg aatagatctg
     1441 agttttttat ccttgtcatc ccctttcaga cctcccagtt cagattctct gagattcagt
     1501 tctacctgat agttttcaaa cttttcctca cggctggtaa tcgcatcata gtactgtccg
     1561 taagttttct ttttcagatc ttcaaacttc ttaaccctgt cgtctccgga aagctttttg
     1621 ttcagagaat tgaaattctt cgtagcttct acaaaaccga cctgagattc ttccattccg
     1681 aataccaaag atgcatcctc ttcaccgagg atttcctttc tctttttctt aataagatca
     1741 tatctttctt caaaactgag atccttcggc atttcattct gcttcatttc tttttcaaat
     1801 ttaaaatact tagagaagag ctgtttcaaa ttttcttttt caggagaatt gtagtttttt
     1861 tcgatgtatt caaggattgc actgtcgcac tgatcaccgg tgtaattctc cggacagttt
     1921 cttctcatcg cccaaagttc ccagacgaaa tttatctttc ctgatttaag gccggtcatc
     1981 agatcattat aattcaatgc acctccgctg ctctgtattg cagatgtggc ttcagtaaaa
     2041 actgaaccag ggtcgcccaa aggataaaga ttctgatcat ttgtgctcat atcatttttc
     2101 ttgcctcctt tttttccgct gttgccggaa aaaataaaaa aagccgccac tgcagcgaca
     2161 agaattcctg ctaaaatata aatatgcttc ttttgcattg ttacctcgat aattagttta
     2221 aaagggcagg cttttacacc tgcccaagaa gagattaaag acctgctttt gcgttgttgg
     2281 ctattgctaa ataccagcct tgaacatcaa aataagtctg accgctccac tccaggtttg
     2341 ttgcctgaag gtgatcaatc ccagtagcaa accaataagc agaagctccg cccttccatg
     2401 ttccccaagc ctgtgaagaa tatggaacta ctccgtcatt gtctccgcca agtccgtaat
     2461 acagtccccc tgcccaagtg attgggtaag tgagagccat gatcgga
//
LOCUS       DAOQGJ010000412         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26585_length_2506_cov_2.451652, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000412 DAOQGJ010000000
VERSION     DAOQGJ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26585_length_2506_cov_2.451652"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /gene="galE"
                     /locus_tag="PK683_07630"
     CDS             complement(<1..895)
                     /gene="galE"
                     /locus_tag="PK683_07630"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNH08356.1"
                     /translation="MRIAVTGGAGYIGSHIVKELNRRNYEVVVVDTLEKGNRVNLFPQ
                     NEFIQGDIQNEEILKKLFSKKTEAVFHFAAWKAAGESMTSPEKYSLNNLNGTLKLITY
                     MLEKDCRNFIFSSSAAVYGAPQYLPIDENHPLNPENYYGYTKLAIEENLKWYDRLKGL
                     KFAALRYFNAAGYDPEGIVKGLEQTPANLLPIIMETACGMRKEFQIYGEDYDTPDGTC
                     IRDYIHVTDLAEAHVLSLDYLIKNRESLIVNLGSENGYSVKEIVSISEKVVGKKIPHS
                     PTGRRAGDPAKLLASSAKARNL"
     gene            complement(905..1684)
                     /gene="lpxA"
                     /locus_tag="PK683_07635"
     CDS             complement(905..1684)
                     /gene="lpxA"
                     /locus_tag="PK683_07635"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNH08357.1"
                     /translation="MKIHPTAIIDKNAKLGTNVEVGPYSIIEGDVEIGDNCWIESCVK
                     IYSGTRMGKNNKIHHTAIVGGPPQDLSFKLETKTYTIIGDDNVIREGVILHRATKEGK
                     ATTLGNRNYLMGQTHIAHDCILGDDVIMVLNGQIAGHCVIGNKVFISGLTGLHQFCKV
                     GDYAMLAGCSKIVKDVPPFVTIDGNPATVIGLNSVGLKRAGFAGPAREKIKQTYKTIY
                     HSGMNVSQALKVLKEQNDPDPNVQYIVRFFEESDRGVTDHR"
     gene            1784..>2506
                     /locus_tag="PK683_07640"
     CDS             1784..>2506
                     /locus_tag="PK683_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH08358.1"
                     /translation="MEYSKEEIQKGLEFIRRLSEDGGLFASLSEEERISVLTDCGKIS
                     RPNADEIRKRNREVRKAKRREKRTAEKKARAATGIREARKDAVFKAPLKLEGPKNDDP
                     EMELKSPRNCYVCKKEYTKLHFFYDSMCRECGDFNYEKRFQRVPLHGKTALITGARVK
                     IGYHCALMMLRSGAKVIVTTRFPADSAGRFAKEEDFSDWADRLEIHGLDLRHTPSVEI
                     FCNYIIHKEERLDILINNAAQTV"
BASE COUNT          649 a          587 c          556 g          714 t
ORIGIN      
        1 ggagattacg ggcctttgca gaggaggcaa gaagtttcgc aggatcacct gcacgtcttc
       61 cagtcggact gtgaggaatt tttttcccta cgaccttttc agaaatgctt acgatctcct
      121 tcactgagta tccgttttcc gatcccagat ttacaatgag cgactctctg tttttgatca
      181 gatagtccag actcagcaca tgggcttctg cgaggtctgt cacatgaata tagtctctga
      241 tgcaggttcc gtcaggagta tcatagtcct ctccgtaaat ctgaaactct tttctcattc
      301 cgcaggcagt ttccattatg atcggaagca gatttgcagg agtctgctcg agtcctttca
      361 caattccctc aggatcatac ccggcggcat taaaatatct gagtgccgca aatttcagac
      421 ctttcagcct gtcgtaccat ttcagatttt cctcgattgc aagtttggta tacccgtaat
      481 agttttccgg attcaaagga tggttctcat caatcggaag atactgaggc gccccgtaca
      541 ctgcagctga ggaagagaaa ataaaatttc tgcagtcttt ttcgagcatg tacgtaatca
      601 gcttcaatgt gccgttcaga ttgttcagag aatatttttc aggagaagtc atggattctc
      661 ctgcagcctt ccatgcggca aaatgaaaga ctgcttctgt ctttttggaa aacagtttct
      721 tcagaatttc ttcattctgt atgtcgcctt ggatgaattc gttctgcgga aacagattga
      781 ctctgtttcc tttttcgagt gtgtccacaa cgacgacttc ataatttctt ctgttgagtt
      841 cctttactat atgactgcct atgtacccgg caccgcctgt gactgctatt ctcataattt
      901 ttccttatct gtggtctgtg acgcctctgt cgctttcttc aaagaaacgg acgatgtact
      961 gcacgttcgg atcaggatca ttctgctcct tcagcacttt gagggcctga gatacattca
     1021 ttcctgaatg ataaattgtt ttataagtct gcttaatttt ttctctcgca gggcctgcaa
     1081 atccggctct tttcagaccc acgctgttca gaccgatcac agtggcaggg tttccgtcaa
     1141 ttgtgacgaa agggggcaca tcctttacga tcttggagca tcctgcaagc attgcatagt
     1201 ctcctacctt gcagaactgg tgcagtcctg tcagccctga aatgaacacc ttgtttccta
     1261 tcacgcagtg acctgcaatc tgtccgttca gaaccatgat gacatcatcc ccgagtatgc
     1321 agtcgtgtgc aatatgagtc tgtcccatca gataatttct gtttccgaga gtggttgcct
     1381 ttccttcctt tgtcgccctg tgcagaatga ctccttcacg gatcacattg tcatcgccga
     1441 taattgtata tgtttttgtc tccagcttga aacttaaatc ctgaggaggc cccccgacga
     1501 tcgcagtgtg atggatcttg ttgttctttc ccattctggt tcctgaataa atctttacac
     1561 agctttctat ccagcagttg tctccgatct ccacatctcc ctcaatgatg gaatagggac
     1621 cgacttccac attcgtcccg agctttgcat tcttgtctat aatcgcagta ggatgtatct
     1681 tcataaaagc agtctgaatt tgagggaagc aagtgcaagc aggatttttg gatgctgaat
     1741 tgtgaccgca gtaaattttg gtcatagatc aggccggtta aaaatggaat actcgaaaga
     1801 agaaatacag aagggactcg aattcatccg aaggctctcg gaagacgggg gactgtttgc
     1861 atctctttcc gaggaggaaa gaatctctgt actgactgac tgcggtaaaa tttcgagacc
     1921 gaatgcggac gagatacgga aaagaaacag ggaagtccgg aaggcaaaaa gaagagaaaa
     1981 aagaaccgct gaaaaaaagg ccagggcagc cacaggaatc cgtgaagcgc gaaaggatgc
     2041 agttttcaag gctcccctga aactggaagg acccaaaaac gatgatcctg aaatggagct
     2101 caaatccccc cgcaactgtt atgtctgcaa aaaggaatac acaaaacttc atttcttcta
     2161 cgattccatg tgcagggaat gcggagattt caattatgaa aaaagatttc agagagtccc
     2221 tcttcacggg aaaactgctc tcatcacagg agcccgcgta aagatcggct atcactgtgc
     2281 tctgatgatg ctccgctccg gcgcaaaggt cattgtgact acacgttttc ctgcagactc
     2341 cgccggaagg tttgcaaagg aagaggattt ttccgactgg gcggacagac tggaaattca
     2401 cggactcgat ctcagacata ctccgagtgt ggagatattc tgtaattata ttatacataa
     2461 agaagaaaga ctggatattc tcatcaacaa tgctgctcag acagtc
//
LOCUS       DAOQGJ010000413         2504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26605_length_2504_cov_3.466313, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000413 DAOQGJ010000000
VERSION     DAOQGJ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26605_length_2504_cov_3.466313"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>2504
                     /locus_tag="PK683_07645"
     CDS             24..>2504
                     /locus_tag="PK683_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HNH08359.1"
                     /translation="MKEDFRPDPDQLLEAIKKEEEKNRKGKLKIFLGMAAGVGKTYAM
                     LSEGARLRLNDLVNVIIGVVETHGRKETEELSFVIPSIPRKKIPYKGNEFQELDLDAV
                     LELKPDIVLIDELAHTNIPGARHSKRYQDVQEILDAGINVYTTLNIQHVESQTDLVAK
                     MTGITVYETVPDSIIDLADEIELIDLPVTELMKRLSEGKIYPSSRVETASLNFFKKEN
                     LTALREMALRTAAERVDKQLNEILQFQSEKSNWKGANKILVTVGPSPTSRMVLRSARR
                     IAGSMEAELTAVFVETPGSLKPSEREDLQKNINYARELGAEAVITAAETVAEGILNAV
                     RTRNIYTVITGRPETGFIKSFFFKDLHDRLMAEEPDLNIFIIQGQKKTNERPSKKSFI
                     SDMEPVSGIKEYFQSLLSVALVTLVNLALLNFTGYWSISLIYLMSVLVLALFLGRGPV
                     MAAAVISGFLWNFLFIPPRFTFFIHTFEDFLMLLIYIFAALIAGIFTTKIRQREKLIQ
                     NREKINTYLYRHSEELAVCNTAEEIARVTVRNIKNIFSSECIYAVPENGMKIISSSEI
                     TTFSPKEYSVAQWSFDKKKKAGRFTDTLSSSEGHYIPVQTATSNFGVIGIFFSEEIYS
                     ERYNEFLIDSIAGQTALSLERMQIRDEAVKNKLLYESEKFSRTLLNSISHEIRTPLSQ
                     IKGASSILHEKALYDGQDYSRELTEEIQESVEDLDHIINNLLDMTRLESGKVKLKMDW
                     TDPNDLIQQSIKRTKGKLIHHKVIVDSVSSVPQRMDVSLMEHVLVNLLLNAAIHTKKG
                     TVIKIKEERTGKYYKLIISNNDFPIFRKD"
BASE COUNT          821 a          466 c          540 g          677 t
ORIGIN      
        1 gctgttaaat ctggagctgg acaatgaaag aagattttcg ccctgatccc gatcagcttc
       61 tggaagcaat aaaaaaagaa gaagaaaaga acaggaaagg aaagcttaaa atatttctgg
      121 ggatggctgc aggtgtcgga aagacatatg caatgctttc tgaaggtgcc agactaaggt
      181 tgaacgatct tgtgaacgtg attatcggag tagtggaaac tcatggaaga aaagaaacag
      241 aagagctttc ctttgtcatt ccatccattc caagaaaaaa aattccctac aaaggaaacg
      301 aatttcagga gctggatctg gatgcagttc ttgagctgaa accggacatt gtgctgattg
      361 atgaacttgc acatacgaat attcccggtg caaggcattc taaaagatat caggatgttc
      421 aggagattct ggatgcagga ataaatgttt atacaacgct gaatattcag cacgtagaaa
      481 gtcagactga tctcgttgcc aaaatgacag gcattactgt atatgaaaca gttcctgatt
      541 ccatcattga tctggctgat gaaattgagc tgattgatct gcctgtaacg gaactgatga
      601 aaagactttc tgaaggaaaa atatatccgt cttcaagagt cgagacagcc tctctgaatt
      661 ttttcaaaaa ggaaaacctc actgcccttc gggaaatggc ccttagaact gctgctgaaa
      721 gagtagacaa gcagctgaat gagattcttc agtttcagtc tgaaaaaagc aattggaaag
      781 gcgcaaataa aattcttgtc accgtcggtc cgagtccgac gtcacgaatg gtgctccgtt
      841 ctgcaagaag gattgccgga agtatggagg ctgaactgac agcggttttt gtggaaacgc
      901 ccggaagtct gaaaccctct gaaagagagg atctgcagaa aaatatcaac tatgcgagag
      961 agcttggcgc agaggctgtg atcacagctg cagaaacagt tgcagaggga atactgaatg
     1021 cagtcagaac aagaaatata tacactgtaa ttacaggaag acctgagact ggatttatta
     1081 aaagtttctt ttttaaggac ctgcatgaca ggctgatggc agaagaaccg gatctgaata
     1141 ttttcattat tcagggccag aaaaaaacaa atgaaaggcc ttccaaaaaa tcatttattt
     1201 ccgatatgga accggtttca gggataaagg agtactttca gtctcttctc tctgttgcac
     1261 ttgtcacctt ggtaaatctc gctctgctga actttacagg atactggtcg atatctctga
     1321 tttatctcat gagtgttctt gtactggctc ttttccttgg acgaggtcca gtgatggctg
     1381 cagcagtcat cagcggattc ctgtggaatt ttctcttcat tcctcccagg ttcacttttt
     1441 ttatccacac atttgaagat tttttaatgc ttcttattta tattttcgcc gcactgattg
     1501 ccggaatatt cactaccaaa atccgtcaga gagagaagct gattcagaac agagaaaaaa
     1561 tcaataccta tctgtaccgt cattcagagg aactggcagt atgcaataca gcagaagaga
     1621 ttgccagggt gacagtgagg aatataaaaa atatcttttc ctctgaatgc atctatgctg
     1681 ttcctgagaa cggaatgaaa atcatttcca gttcagaaat aactacattc tctcctaaag
     1741 aatattcagt tgctcagtgg tcattcgaca agaaaaagaa ggctgggaga tttactgata
     1801 ctctgtcctc ttctgaagga cactatattc ctgtacagac agccacatcc aatttcgggg
     1861 ttattggaat attcttttcc gaggaaattt attctgaaag atataatgaa tttttaattg
     1921 attctattgc aggacagact gctctttctc tggagagaat gcagataagg gatgaagcgg
     1981 tcaaaaataa acttctatat gaatcggaaa aattttccag aacactttta aattccattt
     2041 cccatgaaat aaggacacct ctttcgcaga tcaaaggagc ctccagtatt cttcatgaaa
     2101 aagctctcta tgacggtcaa gattactcaa gagaactgac cgaagaaatt caggaatctg
     2161 ttgaagatct ggatcatatc ataaacaatc ttctggatat gaccagactg gaatccggaa
     2221 aggtaaaact gaaaatggac tggactgacc caaatgatct gattcagcag tcaataaaaa
     2281 gaacaaaagg gaaactgatt catcataagg taatcgttga ttcagtgagt tcagttccgc
     2341 agaggatgga tgtatccctg atggaacatg ttttagtcaa tctgcttctg aacgcggcaa
     2401 tacatacaaa gaaaggaact gtgattaaaa tcaaggaaga aaggactgga aaatactata
     2461 aacttattat cagcaataat gattttccaa ttttcagaaa agac
//
LOCUS       DAOQGJ010000414         2504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26611_length_2504_cov_2.813802, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000414 DAOQGJ010000000
VERSION     DAOQGJ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26611_length_2504_cov_2.813802"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK683_07650"
     CDS             complement(<1..566)
                     /locus_tag="PK683_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08360.1"
                     /translation="MMKKKESAARKEKQESAPEDSVLTLEEEDAKARQKEVRNLKKVL
                     KKCINADGDFDLDVLNESEIQIFMKSVIGYIFEAYSVSSVFYGGLEYRNHKNQWYFPE
                     NLDLPHDPAVECTGYDKCEYEKGRELVRFQPEKGKKIKYDIGSKDYNEDPELFPTLGV
                     IDGVFSSYLRILHTKRLILLNDVFNEDHR"
     gene            complement(621..1298)
                     /locus_tag="PK683_07655"
     CDS             complement(621..1298)
                     /locus_tag="PK683_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08361.1"
                     /translation="MSRKETSLDEILKKVQTSKNLFNLDILTQKETERFFNSLCMYIF
                     EGYELKSVEYDGKLYHNQPNMWDSETHPGFAHDPVTPCTGYDECEFENGKALDRFLPE
                     KGKKIKYNIEFIGLDGAEEDDEDLGVMAAVFKHENIMIHSKPLVIFNREFNDDHRGSD
                     HTRIILDFPKRVEFKPQKTKMEKFIDGLFRLKSHKFENYYEMYMGTKVRFKEEEIFVS
                     VRFDHGS"
     gene            complement(1491..2498)
                     /locus_tag="PK683_07660"
     CDS             complement(1491..2498)
                     /locus_tag="PK683_07660"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH08362.1"
                     /translation="MEIKLTLYEKLALKWIHFQKELEKIHKIGQTRLSFLFIPHNNDR
                     IFRIEASYYLMAFLSAMGVSILILCLYFSAAFLIFREKSKLISEAGHKQKKTFQHYAK
                     IAKEMENSMEDLEDNLENLNKVTWGKFSRDARPSLTEELEDDTVAAGVFYTEPEMKTN
                     MKIYTSTVEKFKNLHIQLQKLKPLFLNASDYLETRESIVQSMPRGRPLGPGVGFIAST
                     YGGREDPVKGGGEHHNGVDFAAPNGTPIYATAPAVVAEASYSESSFGNFVKLHHPNGY
                     FTLYAHCSELKVKKGEKVSRGDLIALVGSTGKSTGTHLHYEVHIGMDPPYNPQEFINL
                     D"
BASE COUNT          678 a          577 c          440 g          809 t
ORIGIN      
        1 cgatgatcct cattaaaaac gtcattcagc agtataagtc tttttgtatg cagtatacgc
       61 aaataggagg aaaaaacgcc gtctatcacg ccgagagtcg gaaacagttc aggatcttcg
      121 ttataatcct tcgatccgat atcgtatttg attttctttc ctttctccgg ctggaatctc
      181 accagttccc tgcccttttc atattcacat ttatcgtatc ctgtgcactc cactgcagga
      241 tcatgcggca aatccaagtt ttcaggaaaa taccattgat tcttatgatt gcggtattcc
      301 agaccgccat aaaaaacaga agacacagaa tatgcttcaa atatataccc gatgacagat
      361 ttcataaaaa tctgaatttc agattcattt agaacatcca gatcaaaatc tccatctgca
      421 ttgatgcact ttttcagaac ctttttcaag ttacggactt ctttctgtct tgcctttgca
      481 tcctcttctt ccagtgtcag tacagagtct tccggtgccg actcctgctt ctctttcctt
      541 gctgcggatt cctttttttt catcatatcc atccttctga ctgcagaaat taataaaagt
      601 gattattata atattattta ttatgatccg tgatcaaacc gcacagaaac aaaaatctct
      661 tcttccttga accttacttt tgtccccata tacatctcat aatagttttc aaacttgtga
      721 cttttcagtc taaaaagacc atcgataaat ttctccattt tcgttttttg gggtttgaat
      781 tccaccctct tgggaaagtc cagaatgatt cgagtgtgat cgcttcccct gtgatcgtca
      841 ttgaattccc tgttgaagat cacaagcggt tttgaatgga tcattatatt ttcatgttta
      901 aatacagcag ccatgacacc cagatcttca tcatcttcct cggcaccgtc cagaccgatg
      961 aactcaatat tgtacttgat tttctttcct ttttcgggta aaaaacgatc cagtgctttt
     1021 ccgttctcaa attcacattc atcatatcct gtgcagggcg ttacaggatc atgcgcgaaa
     1081 cccggatgag tttcggaatc ccacatattc ggctgattat gatagagctt tccgtcatat
     1141 tccacagact tcagttcata accctcaaaa atatacatac acagactgtt aaaaaatcgt
     1201 tctgtttctt tttgggtcaa gatatccaaa ttaaaaagat ttttggaagt ctgcactttt
     1261 ttaagaattt catccagact tgtttccttt ctgctcattt tcggttccct gaagtaaatg
     1321 cgatacagta tcaaaacctg tcaaaatatg tcaattcatt tctcgagaaa ctggaaagcg
     1381 gtcaaaaatc tgaaagagaa acttatgtct gaatggattt gtactgaaag cgcaatggcc
     1441 tggaatgaat ttgcctgcag tcctgttttg aatgaacaga agtccgcata ttaatccaag
     1501 ttgatgaatt cctgcgggtt atatgggggg tccattccta tatgaacctc atagtgaaga
     1561 tgagtccctg tggacttccc agtacttcct acaagtgcga tcagatcccc tctgctgact
     1621 ttctcaccct tcttcacttt cagctcagag cagtgagcat acaaggtaaa gtatccgttg
     1681 ggatgatgaa gctttacaaa atttccgaaa ctggattctg aataactggc ttctgcaacg
     1741 acggcaggag cagttgcata aataggagtt ccgttcggag cggcaaagtc cactccgtta
     1801 tgatgttcgc ccccgccttt taccggatcc tcccgacccc cgtatgtaga ggcaatgaaa
     1861 ccgacccccg gccctagagg acgtccccta ggcatcgact gaacaatact ctctcttgtt
     1921 tccagataat cggacgcatt tagaaaaagc ggcttcagtt tctgaagctg aatatgcaga
     1981 tttttaaact tctctacggt agacgtatag attttcatat ttgtcttcat ttcaggctca
     2041 gtataaaata caccggctgc aacagtatca tcctcaagtt cttccgtcag actcggtctc
     2101 gcatctcttg aaaattttcc ccaagtcacc ttgttcagat tttccaggtt gtcctcaaga
     2161 tcctccatac tgttttccat ttcttttgca atttttgcat agtgctgaaa tgtcttcttc
     2221 tgcttatgtc ccgcttctga tatgagtttg gacttttctc tgaaaatcag aaaggctgca
     2281 ctgaagtaca gacagagaat cagaatgctg actcccattg cacttaagaa agccatcaga
     2341 tagtaggagg cttctattct gaaaatcctg tcattattat gaggaataaa aagaaaactg
     2401 agtctagtct gaccgatctt atggattttc tccagttctt tctggaaatg aatccacttc
     2461 agtgccagtt tttcatagag tgtaagtttt atttccattt accc
//
LOCUS       DAOQGJ010000415         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26622_length_2503_cov_3.079248, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000415 DAOQGJ010000000
VERSION     DAOQGJ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26622_length_2503_cov_3.079248"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..918)
                     /locus_tag="PK683_07665"
     CDS             complement(19..918)
                     /locus_tag="PK683_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ kinase"
                     /protein_id="HNH08363.1"
                     /translation="MRQALIILKKTKFELDEERYPDREFYAKITSLQNNCYDRIYHSH
                     LRQLESRKILQEIFGPENCVFRDEFDRKNPADYEYLIALGGDNHFTYVAHHANGTKVI
                     GCNSDVKTSRGVLLGFSPEMLRDAVENNFQDTEVVEWAMIYGRLEYPEGTTLETVGCV
                     NEISIRNMSPDLTSRYLLRYRDSQEEQKSSGLLLYTGAGSTGWYSSCLGLSGNEQQKF
                     PKDAPYFGVFARELSHRARLNFKLNDFIANGSVTVVSEMNGGISIDSLPERIYHFPPG
                     TIGTFQLSSHKLKTVVPKQENSI"
     gene            1044..1982
                     /locus_tag="PK683_07670"
     CDS             1044..1982
                     /locus_tag="PK683_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08364.1"
                     /translation="MTREELLKYIKKLEFENTVLNDEVLAVAKSPYLPQTVARLTYKV
                     VLHREEILKNELRSRVDEKKGSLFEIKNQIRKYAPILGLDPDSIRILVTEAIQNIVEH
                     GYGQYAEIELEINNTGPNPYFKTSFKHGMPEGRKYTLAQIEENAKKGDVTSEEFDFEN
                     PRGRGEFLMKEISDERQIINGVDVDENGNRIHYFKRVMINYKYPDGPRIETSFDEIRE
                     ELDRLDGDEVVCYFHVDHKKNKLDTITVVTNKNREHTVRKMMEDAGFTLFHKDSYSKT
                     IFSSFKPGREYTESEINTLFSEVRKVVSAEVLHKMT"
     gene            2240..>2503
                     /locus_tag="PK683_07675"
     CDS             2240..>2503
                     /locus_tag="PK683_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH08365.1"
                     /translation="MKENYWQNKTAVVTGGSSGIGEALVKKLSSVKCRIINVDRNPAT
                     VSSDKAEIHSVICDLSLPDSVEKASAEIAKLTDSSTATVAKVKR"
BASE COUNT          804 a          516 c          541 g          642 t
ORIGIN      
        1 gaaaaagttc ttctgaattc atatactgtt ctcctgtttt ggaaccactg tctttaattt
       61 atgcgaggac agctgaaatg tgcctattgt gccaggcgga aaatgataaa ttctttccgg
      121 aagagaatcg atggagattc ctccgttcat ttctgaaacg actgtcactg atccgtttgc
      181 tataaaatca ttcagcttaa aattcagtct tgccctgtgg ctcagttctc ttgcaaatac
      241 tccgaagtag ggagcatcct ttggaaattt ctgctgttca tttccggaca aaccgagaca
      301 ggaggaatac catcccgtgg aaccggcccc tgtatacaga agcagacctg agcttttctg
      361 ttcttcctgt gaatccctgt accgaagcag atacctgctc gtcagatcag gactcatatt
      421 ccgaatcgag atttcattga cgcatcccac tgtctcaagg gttgttcctt caggatattc
      481 cagtcttccg taaatcattg cccattccac gacctcagtg tcctggaaat tattttctac
      541 tgcatctctc agcatttcag gactgaaacc gagcagaacg cctcttgatg tcttcacatc
      601 tgaattgcac ccgatgactt ttgtaccgtt tgcatgatga gcgacatacg tgaaatgatt
      661 gtctcctccg agtgcaatca gatattcata gtccgcagga ttctttctgt caaattcatc
      721 tcggaataca cagttttccg gtccgaaaat ttcctgcaga atttttctgc tttccagctg
      781 acgcagatgt gaatgatata ttctgtcata acagttgttc tgaagagaag tgatttttgc
      841 atagaattct ctgtcaggat atctttcctc atcgagttcg aacttcgttt tcttcagaat
      901 aattaaggct tgcctcacaa gaggacagta cgataaaagg aaaggaattg ccaagtaaaa
      961 actgtctcgg cataatggac agaaagcagg catcttcagt cggaaaagtt cgggagaaaa
     1021 aaatgattaa gacaacagat gaaatgacaa gagaagaact tctgaagtac atcaagaagc
     1081 tggaatttga gaacacagtc ttgaatgatg aagtcctggc tgtggcgaaa tctccctatc
     1141 ttccccagac tgttgcccga ctgacctaca aagtagtact tcacagagaa gaaattctga
     1201 aaaacgaact gagatccaga gtagatgaaa aaaaaggctc acttttcgaa atcaaaaatc
     1261 agatacgcaa gtatgcaccg attcttggac ttgaccctga ttccatcaga atcttagtga
     1321 cagaggcaat ccagaatatc gttgagcatg ggtatggtca gtatgcagaa attgaactgg
     1381 aaatcaacaa taccggcccg aacccttatt tcaagacttc attcaaacac ggaatgccgg
     1441 aaggaagaaa atacacattg gcgcagattg aagaaaatgc caagaaaggc gatgtgactt
     1501 cagaagagtt tgattttgaa aaccctagag ggcgcggaga atttctgatg aaagaaattt
     1561 ctgatgaaag acagattatc aacggtgtgg atgtggatga aaacgggaac aggattcact
     1621 atttcaaaag agtgatgatc aactacaaat atcccgacgg accaagaatt gaaacaagtt
     1681 ttgatgaaat cagggaagag cttgaccgtc ttgacggcga tgaagtagtc tgttattttc
     1741 acgtagatca taaaaagaac aaactggata cgattacagt cgtcacaaac aagaacagag
     1801 aacacacagt caggaaaatg atggaagacg caggattcac actgtttcac aaggactcct
     1861 attccaagac gatattcagt tcattcaaac caggaagaga atacacagaa tctgaaatca
     1921 acacactttt ttcagaagtc agaaaggtag tgagtgcaga agttctgcac aagatgacct
     1981 gataaaattg gcgagtgctg ggtggaaaac agattataat agaatttatg gatacagttc
     2041 gaaaaacagg gtatcagggc ttcacagcct gcgggtgcta atccctcgcc atccgagaaa
     2101 gaggctaatc ctcccgtcag ggacaaacct tttcttgctt ttttaaaagc agacgctgaa
     2161 aaaggatatt tgtaactgcg acggctggct ttgaagctag ctgccgccag gctgagaagt
     2221 taccaaaaat ggagaaagaa tgaaagaaaa ttactggcag aacaaaacag cagttgtcac
     2281 gggaggatcc agcggaatag gcgaggctct tgtaaaaaaa ctctcttcag tcaaatgcag
     2341 gattatcaat gtagacagaa atccggcaac tgtatcatcg gataaagcag aaattcattc
     2401 agtcatctgc gatctttctt tacctgattc agtggagaag gcatctgcag aaattgcaaa
     2461 actcaccgac tcatctactg ccactgttgc aaaagtaaaa cgg
//
LOCUS       DAOQGJ010000416         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26692_length_2499_cov_3.683306, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000416 DAOQGJ010000000
VERSION     DAOQGJ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26692_length_2499_cov_3.683306"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..912)
                     /locus_tag="PK683_07680"
     CDS             complement(211..912)
                     /locus_tag="PK683_07680"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08366.1"
                     /translation="MIKKYNIICSVFIGTVFLLGCSQGNDLPAGVEKGAEFDRKINLY
                     KLVQNGKRKLWSDSGKLYAVIEVNASDLENGNAETYMPATGAVLSRGNYKDGQKDGLW
                     FWYFPDGKVYYKQEFAFGKKRSYWIETNLIGNEHGLYERYYFDGKLEEKGQFDSGYKT
                     GEWVKYYRNGNIEYKGKFLKDKRVGKWFQYFSDGKTESEELYDEKGKLIERKTFFPDG
                     KPRCIQKQNEPYQCA"
     gene            complement(914..1693)
                     /locus_tag="PK683_07685"
     CDS             complement(914..1693)
                     /locus_tag="PK683_07685"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNH08367.1"
                     /translation="MSEKSRILITGASGVLGAELCRILDKRNIPYTGAGRSESSRQNW
                     VSIDLKTGKNLSEAVKDKDIVFHLASDTKNFSRESDVDGTEKLCTAALNAGVRYFIYI
                     SIVGIDKVPYKYYEIKLETEQKIVNSGLQYSILRAVQFHDFMDMLLEKFTKFPVVFLP
                     GKLKFQPIETSSVAEELLRIAENPPLNSVRNLGGKEILPLSEIMRIWLKAQGRKRLVI
                     PVPALGKMMKSLASGGLTCTEKSENSITWSEWVMRKYSQGK"
     gene            complement(1686..2267)
                     /gene="pyrE"
                     /locus_tag="PK683_07690"
     CDS             complement(1686..2267)
                     /gene="pyrE"
                     /locus_tag="PK683_07690"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNH08368.1"
                     /translation="MLEQKKKLLFDLIKNCAYRYSEQEFTLASGKKSRHYFNCKEILL
                     YPDRLSLLADYIVNVHIPENGITPEAVGGLTMGADPICYSVALKFYANGKTVYPLIVR
                     KEAKDHGTKKEIEGALSTVKSCLAVDDVITTGGSTLKAVEALRRAGLSVTQGICILDR
                     QEGGFEALKENGVQMYSLFKKSDFFTDGTDAFE"
     gene            complement(2288..>2499)
                     /locus_tag="PK683_07695"
     CDS             complement(2288..>2499)
                     /locus_tag="PK683_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08369.1"
                     /translation="SDETVKRQMDLEEEVLMEMQDLERKIEEKEKTIQKITEEKDKTI
                     EEKDKTIEEKDRIIEELKQQLKKTI"
BASE COUNT          603 a          597 c          458 g          841 t
ORIGIN      
        1 ccgtaggctt tgcagaactt cgcctctttg ctactttctt tttttgcagc tttttcacaa
       61 aagcgagaaa aaaagaaagt agcttaagat attataaaga aaagatcatt ataaaaacag
      121 agagcactga gctccgccga agtgtgagaa gtgcttttac acagtccgtg agttaaggct
      181 gttctgcaaa tcacgggtac taagcgaagc ctacgcacac tgataaggct cgttctgctt
      241 ctgaatgcat ctgggttttc cgtccggaaa gaatgttttt ctttcgatga gttttccttt
      301 ctcatcataa agttcttctg attctgtctt tccgtctgaa aaatactgga accattttcc
      361 tactctttta tctttcagga attttccctt gtattcaata tttccgtttc tgtagtattt
      421 gacccattcc cctgttttgt atcctgaatc aaactgaccc ttctcttcaa gttttccgtc
      481 gaaataatag cgttcataca gaccgtgttc atttccgatc agatttgttt caatccagta
      541 gcttcttttc tttccgaatg caaattcctg cttatagtat actttcccgt cagggaagta
      601 ccagaaccag agtccgtctt tctgaccgtc cttataattt cccctgctga gaactgcccc
      661 tgtggcaggc atataggtct ctgcatttcc gttttccagg tctgaggcat tgacttcaat
      721 gactgcatac agtttccctg aatccgacca gagttttctt tttccgttct gcacgagctt
      781 atacagattg atctttctgt caaattctgc ccccttctcc actcccgcag gcagatcatt
      841 tccctgagag cagccgagaa ggaacacagt tccgataaac acagagcaaa ttatattata
      901 tttttttatc atttcatttt ccttgagaat attttctcat gacccattct gaccaggtga
      961 tgctgttttc cgatttttca gtacaggtca gtcctcctga tgcaaggctt ttcatcattt
     1021 ttcccagtgc aggaacagga atgaccagtc tcttacggcc ctgtgctttc agccagattc
     1081 tcatgatttc agaaagaggg aggatttctt ttccgcccag atttctgact gaattcagag
     1141 gagggttttc tgcaattctc aaaagttctt ctgccacaga ggatgtttca ataggctgaa
     1201 acttcagttt tccaggcagg aatacgacag gaaactttgt gaacttttcc agaagcatat
     1261 ccataaaatc atgaaactgc actgcacgca atatggaata ttgcagtcct gaatttacga
     1321 tcttctgttc agtttccagt ttgatttcat agtacttata tggaaccttg tcaattccga
     1381 caatggatat ataaataaaa taccgtactc ctgcattcag tgctgcagtg cagagttttt
     1441 ctgttccgtc cacatccgac tctctgctga aattctttgt gtcggatgca agatgaaata
     1501 cgatgtcctt atcttttaca gcctctgaaa gatttttgcc tgttttcaga tctatcgata
     1561 cccagttctg cctcgaagat tcagacctgc ctgcacctgt gtaaggaatg tttcttttgt
     1621 ccagaattct gcagagctct gctcccagaa caccggacgc accggtgatc aggattctgc
     1681 tcttttcact caaaggcatc cgttccatcc gtaaagaaat ccgatttttt gaagagggaa
     1741 tacatctgca caccgttttc tttcagcgcc tcaaatccgc cttcctgtct gtccagaatg
     1801 cagattccct gagtcacgct gagtcctgca cggcggagtg cttccactgc tttcagagtg
     1861 gaacctcctg tagtgattac atcgtccaca gccagacagc tttttaccgt gctcaaggcg
     1921 ccctctattt ctttttttgt accgtgatcc tttgcttcct ttctgacaat cagaggatac
     1981 actgtttttc cgtttgcata aaacttcagt gccactgaat aacagatagg atcggcaccc
     2041 atagtcagac ctccaacagc ttcaggagta attccgtttt cagggatatg tacattcaca
     2101 atatagtctg ccagaagaga aagcctgtcc ggatacagaa ggatttcctt gcagttgaaa
     2161 taatgtctgg attttttccc tgaagcaagt gtaaattcct gctcagaata tctgtacgca
     2221 cagtttttga taagatcaaa aagaagtttt tttttctgtt ccaatataag tctccctcag
     2281 tctgcagtca tatagttttc tttaactgct gtttcaactc ttctataatt cggtcttttt
     2341 cctcaatggt tttgtctttt tcctcaatgg ttttgtcttt ttcctcagtg atcttctgga
     2401 tagtcttctc cttttcctcg atctttcttt cgagatcctg catttccata agaacctctt
     2461 cttcgagatc catctgccgt ttgacagtct catcggagg
//
LOCUS       DAOQGJ010000417         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26731_length_2497_cov_2.970106, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000417 DAOQGJ010000000
VERSION     DAOQGJ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26731_length_2497_cov_2.970106"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PK683_07700"
     CDS             <1..920
                     /locus_tag="PK683_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08370.1"
                     /translation="KKSRYIPPSGDSQSIRKELKNISFGNSLRMNELADEIQKFYTKL
                     SQISSRDRKFVLFTDAKSGGKDSGLLLTVLQKIKSLGFKTYLISGSFFDYKEAKLYKK
                     LGRAVTAHYELTHFQKIGTVGGFKMVYLKDRRIYFENGERETSYGADFREMQSIPEGK
                     VYSFVEFPHPGNFSEVYSKIYSEKVLEKSRVKSDIKENLDSILHHKSSASGKVSRRVL
                     VKSGGQAVWISVNDLNDSFEGKEVSFHTVFRKEFSNPAGFRNVPESTSLYNDSTPLLL
                     VLTPSEIRKLLTKRDSVSGFVKGKVLEIR"
     gene            complement(1151..1939)
                     /locus_tag="PK683_07705"
     CDS             complement(1151..1939)
                     /locus_tag="PK683_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNH08371.1"
                     /translation="MKLFLNFLLSVSISFCSSGAYKADRYQYDESRPSTSSSIAGLID
                     QEKGSASAAAADTVQQNKMIVYNATVRIKSDQIQDKIAEMTKLAESFQGFVLSMNTNG
                     NMVLKVPAKSFREFLNSVKKSVKEYYEEISASDVSEEYYDTALRLENAKKTRVRLLEL
                     YRQAKNVEDVLKIEAELGKTSETIERMEGRVKFLTDQVSYSRVTVYISPFYHAPPARR
                     EKEYKPGILGYPFYWAYKGLGLLYDGIVWLFITENKEEPEKKGN"
     gene            complement(1960..>2497)
                     /locus_tag="PK683_07710"
     CDS             complement(1960..>2497)
                     /locus_tag="PK683_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08372.1"
                     /translation="HPVRILCCIYMNLRTVSALLILISAGCIGNFRSTGSVKIRLADK
                     SKKERLDTIEIRCPYGYHVVDFQIFYYRNFKPNFGSRGLSRCSFFFDPIHSMNNYMHT
                     SILDLKKLNSSLETMEPARPYLIWQYKNTENKETETIGLMFLIFTKDESLMRNDKELF
                     KIAEKNSPVSLDGYIQSE"
BASE COUNT          708 a          517 c          527 g          745 t
ORIGIN      
        1 caaaaaaaag cagatatatc ccgcccagcg gcgattcaca gagcataagg aaggaactga
       61 aaaacatctc cttcggaaat tctctcagaa tgaacgagct tgctgatgag atccagaagt
      121 tctatacaaa actttcacag atcagctcca gagacaggaa gtttgttctc tttacagatg
      181 caaaatcagg cggaaaggat tctggacttc tgctgactgt gcttcagaaa atcaagtcgc
      241 tcggattcaa aacctatctg atttcagggt cattctttga ttacaaggaa gcaaagcttt
      301 ataaaaaact gggaagggca gtgactgctc attatgagct gactcatttt cagaaaatag
      361 gtacagtcgg cggcttcaaa atggtatact tgaaggatcg aagaatctat tttgagaatg
      421 gagaaagaga aacttcttac ggtgccgact tcagggaaat gcagtccatt ccggaaggga
      481 aggtctactc attcgtggaa tttccccatc cgggaaattt ttcagaagta tattccaaaa
      541 tctattccga gaaggttctc gaaaagagcc gagtcaaatc cgatattaag gaaaacctgg
      601 acagcattct gcatcataag agcagtgcgt caggaaaagt gtcaagaagg gttcttgtca
      661 aaagcggggg acaggcagtc tggatcagtg taaacgatct gaatgattct ttcgaaggaa
      721 aggaagtcag ttttcatact gtgttcagga aggaattctc caatcctgca ggattcagaa
      781 atgtgcccga atcgacctcg ctctataatg acagcactcc gcttcttctt gttctgacac
      841 cgtcagagat caggaaactt ctgaccaaga gagactctgt ttccggtttt gtgaaaggga
      901 aggttctgga gatcagataa tgctgaaata atgtgatttg catgatcagg aaaaaacttc
      961 ctgactgtca cggaaatctc tgctcatctc catccctata aaatggagat aagcagacaa
     1021 tgaggaaaaa aaacagctca ttgttttccc tatgctgctg tttcaggact gtgcaagtct
     1081 tcttcagtta ttacagctgc agagtcttta atgggaaata cgatcattgc atcagaagaa
     1141 ctgcattgat ttaatttcct tttttttccg gttcctcttt gttttcagtg ataaagagcc
     1201 atacgattcc gtcatagagg agaccgagtc ccttgtatgc ccagtaaaac ggatacccca
     1261 gtattcctgg cttgtattct ttttctcttc ttgccggagg tgcatgataa aaaggagata
     1321 tataaacagt caccctcgaa taggaaacct gatctgtcag aaatttaact cttccttcca
     1381 ttctttcaat tgtttccgat gttttgccaa gctctgcttc tatcttcaga acatcttcca
     1441 cattcttagc ctgtctgtac agttcaagga gacgaactct tgtctttttt gcattctcca
     1501 gtctgagagc agtgtcatag tattcttcgc tgacatcact tgcggaaatt tcttcatagt
     1561 attccttgac gctttttttg acggaattca gaaattcgcg aaagcttttg gcagggacct
     1621 tcagtaccat atttccgtta gtattcatgc tcagaacaaa tccctgaaag gattctgcaa
     1681 gcttggtcat ttctgcaatc ttgtcctgga tctgatccga tttgattctg actgttgcat
     1741 tgtacacaat catcttgttc tgctgaacag tatccgcagc ggccgcactg gcactgccct
     1801 tttcctggtc gatcagtcct gcgatggaag aggatgtgct tggacggctc tcatcatact
     1861 gataacggtc tgccttgtag gctccactgc tgcagaatga aatggaaacg gataaaagaa
     1921 aatttaaaaa aagtttcata aatatttgac ctcagttttt tattccgact gaatatatcc
     1981 gtccagtgag acgggagaat ttttttctgc aatcttgaaa agctctttgt cgtttctcat
     2041 cagtgattca tcttttgtaa atatcaggaa catcagtcct atggtttctg tttccttgtt
     2101 ttccgtattt ttatactgcc agatcagata aggtctggca ggctccattg tctccagaga
     2161 actgttgagt tttttcagat caaggatgct cgtatgcata taattattca tactgtggat
     2221 gggatcaaag aaaaagctgc accgcgaaag acctctggat ccaaagttcg gtttgaagtt
     2281 tctgtaatag aaaatctgaa aatccaccac atggtatccg tacgggcatc tgatttcaat
     2341 cgtatccagt ctctcttttt tggatttatc ggcaagtctg atctttacgg agccggtact
     2401 cctgaaattg cctatgcatc cggcagagat taaaataagc aaggcggaaa ctgtcctgag
     2461 attcatataa atacagcata atattctgac agggtgt
//
LOCUS       DAOQGJ010000418         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26746_length_2496_cov_3.744367, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000418 DAOQGJ010000000
VERSION     DAOQGJ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26746_length_2496_cov_3.744367"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2496
                     /locus_tag="PK683_07715"
     CDS             <1..>2496
                     /locus_tag="PK683_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNH08373.1"
                     /translation="MLNDDLKALFAMGAFYNIDVQAELDPEGNVIIIVEVKERPRVEE
                     IEFIGADEVFPSDMKDKMPLKEGEIITPEKVNATKEVIMKKYKDEGYFHAYIKAELGE
                     INPKTNLVKVKFIIDEGDEIPVAKINILGTQNIDPSELMQILDLKEAGLIESGHFKEA
                     AYETDKQKIVAYMKTKGYRDADLVPEGTGWEIHWENPKLKDKRAIIVNFKVFEGDQYF
                     FNGYSLSHDLTEDGKGNPVFLNKENKENLQPDKSKWKPIYEAKFLENLFEFTDSDTGV
                     IFDEGKFQRDKGVINELYSAKGYIFAQVVTRNRTITLDSDTLSSYESCSVYKGNQTEC
                     EEQNKKLNIKKLRELLTKKPEMKGKKFEHVDFIVRENYLAYIENIIIKGNKKTQDRVI
                     RRELLFKPGDLFNSALVNRSRERIFNLGYFKEVNFNSRPGSDETKMNLIIDLLEQPTG
                     TISMGGGYGTISGFSIFTELGENNLNGTGQKISGRVEFGPLRRFFQLSWTEPWIYNRP
                     WALTLSMFYSSRTINVGAASITDSGTGAIKERASYDREGIGFSIGVAHRIFINWTHFH
                     RYSPSFFQSTNATSLADDSIRAEVNRGWQFRSEITNGIGYDSRDNIFTPTSGLNAYLA
                     VSNVGQYLGGQSHFDRYNPVFEFYHSWFDYTFGGMIRSNALRRWKVVQEFRSSSVFTY
                     ERPPKFGRAAGGIGNLQFDEIEKQRNPFIQQQDLQFLGGYESLRGWNFQDVLYPREWR
                     NGANHRMLFSSELRFPIEPSLLWLVAFLDAGALYEEVGRWTGTRRDNARNYQTALWQT
                     LGTMSPADAYLRANFEPYSLRRLTPDQNPFEKD"
BASE COUNT          784 a          452 c          600 g          660 t
ORIGIN      
        1 aaatgctcaa cgatgatctg aaggcgcttt ttgcaatggg tgcattctat aatatagatg
       61 tgcaggctga acttgatccg gagggcaatg tcatcatcat tgtggaagtg aaggaaagac
      121 cgagggtaga agagatcgaa tttatcggag ccgatgaggt ctttccaagt gatatgaagg
      181 acaaaatgcc ccttaaggag ggagaaatta ttactcccga aaaagtgaat gcaacgaaag
      241 aagtcatcat gaaaaaatac aaggatgaag gctattttca tgcatacata aaagcggagc
      301 tgggagaaat caatcctaag acaaatctgg tcaaggtaaa gtttattatt gatgagggag
      361 atgaaatccc tgtcgcaaaa atcaatattc taggcacaca gaacattgat ccttccgaac
      421 tgatgcagat cctggatctg aaagaagcag gtctcattga gagcggtcat ttcaaggaag
      481 ctgcgtatga aacagacaag cagaaaattg tagcctatat gaaaaccaaa ggctacaggg
      541 atgcagatct tgtgcctgaa ggtaccggat gggaaattca ttgggaaaat ccaaagctca
      601 aggataaaag ggccatcatt gtaaacttta aggtttttga gggggatcag tattttttca
      661 acggttacag tctctctcat gacctgactg aggatggaaa aggaaatcct gtctttctga
      721 ataaggagaa taaggaaaat cttcagccgg ataaatctaa atggaagccg atatacgagg
      781 caaaatttct ggaaaatctg tttgagttta ctgacagcga tacaggagtc attttcgatg
      841 aaggaaaatt tcaaagagac aaaggagtca tcaatgagct ttacagtgca aaaggttata
      901 tttttgcaca ggtagtgact aggaacagaa cgatcactct ggattcagat accctgtctt
      961 cttatgaaag ctgctctgta tacaaaggaa atcagactga atgcgaagag cagaataaga
     1021 aactcaacat aaaaaaactc cgtgagctgc tgacaaaaaa accggaaatg aagggcaaaa
     1081 aatttgagca tgtggatttc attgtccgtg aaaactatct ggcttatata gaaaatatca
     1141 ttatcaaggg aaataaaaag acacaggaca gagtcattcg aagagagctt ctgttcaagc
     1201 ctggagatct ttttaattct gctttggtga acaggtccag ggagagaatc ttcaacctag
     1261 gttattttaa ggaagtcaac tttaactcta gacccggttc agatgaaacc aaaatgaatc
     1321 tgatcattga tcttttggaa cagccgacag gaaccatatc aatgggggga ggatatggta
     1381 ccatttccgg tttcagtata ttcactgaac ttggtgaaaa caatctgaac ggaactggtc
     1441 aaaaaatttc gggaagggtg gagtttggtc ctctcagaag atttttccag ctctcatgga
     1501 ctgaaccgtg gatttataac agaccttggg cacttactct gagtatgttc tattcatcca
     1561 gaacaattaa tgtgggggct gcttccatta cagacagtgg gacgggtgca attaaggaac
     1621 gagcctctta tgaccgggaa gggatcggtt tttccatcgg tgttgctcat agaattttta
     1681 tcaactggac ccactttcac aggtactctc ccagtttttt ccagtccacg aatgcgacat
     1741 cacttgccga tgattccatt cgtgctgagg tgaacagggg ctggcagttc agatcagaga
     1801 ttactaatgg tatcggatac gattcaagag ataatatatt tacacctact tccggactga
     1861 acgcttattt ggctgtttcg aatgtaggtc agtatctggg agggcagagt cattttgacc
     1921 gttacaatcc tgtatttgaa ttttatcatt cctggtttga ttatactttc ggaggtatga
     1981 ttcgttccaa tgctctccga agatggaaag tcgtgcagga attcagatct tccagtgtat
     2041 ttacttatga gaggccgcca aaattcggaa gggcagccgg tggaatcggc aaccttcagt
     2101 ttgacgagat tgaaaagcag agaaatccat tcatccagca gcaggatctt cagtttttgg
     2161 ggggatatga atctctcaga ggatggaatt ttcaggatgt actctatcct agagaatggc
     2221 gaaacggtgc aaatcacagg atgcttttca gttctgaact tcgttttccg attgaaccga
     2281 gtcttttatg gctcgtggca tttctcgatg cgggagcact gtatgaggaa gtggggagat
     2341 ggacgggaac aagaagagac aatgcaagaa actaccagac agctctttgg cagacattgg
     2401 gaacaatgag tcctgctgat gcgtatcttc gtgcaaattt tgaaccgtat tcactcagac
     2461 gtcttactcc cgaccaaaat ccgttcgaaa aggata
//
LOCUS       DAOQGJ010000419         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26761_length_2496_cov_2.504302, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000419 DAOQGJ010000000
VERSION     DAOQGJ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26761_length_2496_cov_2.504302"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PK683_07720"
     CDS             <1..622
                     /locus_tag="PK683_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08374.1"
                     /translation="YVSDELKKAQRKLLSDFRHLGAETEIIPPFPEFRDSFLIWSSVL
                     SEKNTSSFREMLGNGKKISPVLELLKWTFGSSDHMLPCILLSLIEDLPGHIPEYSKHF
                     LGQCFSLKNSLENLLGRDGLLVYPSYSVPAPLHNEPLFRLFDWVYTGIFNALEFPSTQ
                     IPLGLGKQGLPLGVQIVSVRENDALTMAAAAESERMGNRWVMPDRF"
     gene            652..2313
                     /locus_tag="PK683_07725"
     CDS             652..2313
                     /locus_tag="PK683_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH08375.1"
                     /translation="MNRKLTDSDIFRIGFLEVLRETVALFSERPAVIYNEKKYSYHYL
                     WNSALCFSEFLKSSTSPFTGLCFQESPEYIQTLLSVWMAGRPFLTLDRNISNERIMKY
                     MKISGSSEILSDCTERFREENVSAVRPLFSDRDSEKNIPLFDPERSAYAVCSSGSSGE
                     PKVIHILHRGILNFLIQQIEVFQMNEKSRILMNTPLGFDASISEIGVSLLSGAAVCIS
                     SKDLKNPRILMKEADRMSVTHLYIPPSLLPFISTDMIPDSLETVFFGGEESSPEKVKQ
                     FASRLRMINIYGPAETTVCASMAVCGTEWKEQNIGRPIKNTEWILMNAEGRTDENRGE
                     ICISGTGLAKGYIGQSRLNESKFVFYNDKIYYRTGDLAEKTADGSYIFKGRKDRQWKR
                     NGRLIEPAELEKTALSLFPGTRSAALPDSQKRPVLFLEMPDSDTALLRKKIKETLPPW
                     FTPSRIICIQRFPVNINGKTDYSKLSEKLNHPEDNKERRQDICSLAGRLELDICLIQK
                     RKIFLIQEEILSASSELHFFFQRMGMIFRYPIFTKNRHFANLRVY"
BASE COUNT          749 a          528 c          550 g          669 t
ORIGIN      
        1 gtatgtttct gatgaactga aaaaagcaca gagaaaactt ctctcagact tcagacatct
       61 gggtgcagaa acagaaatca ttccgccgtt ccccgaattc agagactctt ttcttatatg
      121 gtcgtcagtt ctatctgaaa agaacaccag ttcgttccgt gaaatgctgg gaaacggaaa
      181 gaaaatatcg ccggttttgg aactgctgaa atggacattc ggaagttcag atcatatgct
      241 tccctgcatc cttctcagtc tgattgaaga tcttcctgga catattcctg agtactcaaa
      301 gcattttctt ggacagtgtt tttcactgaa aaacagtctg gaaaaccttc tgggcagaga
      361 tggattactc gtttatcctt cctactctgt tcccgccccc ctgcataatg aacctctgtt
      421 caggctcttt gactgggtat acacgggaat tttcaatgca ctggaatttc cctccactca
      481 gataccgctc ggactgggaa aacagggact tccattggga gttcagatcg tatccgtaag
      541 ggaaaacgat gcacttacaa tggcggccgc tgccgagtcg gaaagaatgg gaaacagatg
      601 ggtaatgcct gaccgtttct gaaaaattaa attcctgaac agattattat aatgaacaga
      661 aaactgacag attccgatat cttcagaatc ggctttctgg aagttctccg agaaactgtc
      721 gccctttttt ctgagagacc tgcggtcata tataatgaaa aaaaatacag ttatcattat
      781 ctttggaatt cagcactctg tttttctgaa ttcctcaaaa gcagcacctc gccttttacc
      841 ggactatgtt ttcaggaatc accggaatat atccagactc ttctttcggt atggatggcc
      901 ggcaggccgt tcctgacact cgacagaaat atttccaatg aaagaataat gaagtatatg
      961 aagatttcag gttcttctga aattctttca gactgcactg agcgtttccg tgaagaaaac
     1021 gtatcagcag tcagaccttt attttcagac agagactcgg aaaaaaacat tccactcttt
     1081 gatccggaaa gatcggctta tgctgtatgc tcttcgggat cttcaggaga acctaaagtc
     1141 atccatattc ttcacagggg aatactgaat tttctcattc agcagattga agtttttcag
     1201 atgaacgaaa agtccaggat tctgatgaat acaccgctcg gattcgatgc aagcatttct
     1261 gaaatcggag tcagtcttct ctccggagca gccgtctgca tttcatcgaa ggatctgaag
     1321 aatccaagga tactgatgaa agaagctgac cgaatgtccg tgactcatct ttacattccc
     1381 ccttcccttc ttcctttcat aagcactgat atgattcccg attctctcga aactgtcttt
     1441 ttcggaggcg aggaaagcag tcctgaaaaa gtgaaacaat ttgcatcccg actgagaatg
     1501 atcaatattt acggccctgc cgaaacgact gtatgtgcca gtatggcagt ctgcggaact
     1561 gaatggaaag agcagaacat cggcagacct attaaaaata cggaatggat tcttatgaat
     1621 gctgagggca gaaccgatga aaacagaggg gaaatctgca tttcaggaac gggtctcgcg
     1681 aaaggataca tcggtcagag caggctgaat gaatccaaat ttgtttttta taatgataaa
     1741 atatattaca ggacaggcga cttggcagaa aagacagcag acggatctta tatattcaag
     1801 ggaagaaaag accgacagtg gaaaaggaac ggaaggctga ttgaaccggc agagctggaa
     1861 aaaacagctc tgtctctgtt tccgggaaca agatctgctg ctctgcctga ttctcagaaa
     1921 agacctgtcc tctttctgga aatgccggat tcagacacgg cactcttaag aaaaaaaata
     1981 aaggaaaccc ttccgccctg gtttactcca tccagaatca tctgcattca gagattcccg
     2041 gtaaacatca atggaaagac agattattca aagctctctg aaaaattgaa tcatcccgaa
     2101 gacaataagg aaagacggca ggacatttgc tcattggctg ggagactgga actggacatc
     2161 tgcctgattc agaagaggaa gatttttttg attcaggagg agattctgtc agcatcctca
     2221 gaactgcact ttttctttca gagaatgggt atgatctttc ggtatccgat ttttacgaag
     2281 aaccgtcatt tcgcaaactt aagagtttat tgaattctga atcagagcgg ctcatctcaa
     2341 gggaatttct caaaaaagcg gctgaagaat ttctttgcat taaaacacaa tataatgata
     2401 aaagattcaa tgaagagatc agaactgttc ttgtcactgg ggcttcagga ggtcttggat
     2461 cagcggtgcg gtgaccacgg ggtgctcggg gagaat
//
LOCUS       DAOQGJ010000420         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26801_length_2493_cov_2.903610, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000420 DAOQGJ010000000
VERSION     DAOQGJ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26801_length_2493_cov_2.903610"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1100)
                     /gene="ruvB"
                     /locus_tag="PK683_07730"
     CDS             complement(90..1100)
                     /gene="ruvB"
                     /locus_tag="PK683_07730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNH08376.1"
                     /translation="MKERLVNPENKAEEDIFLRPSSLDEFIGQKEILENLKVFIHAAR
                     KRGKALDHVLLSGPPGLGKTTLANIISKEIGSNLTVTSAQVISKGADLARFLTVLSDK
                     DILFIDEIHSLGRGLEEILYPAMEDFQIDIVIGEGITAQSVQIPLQPFTLVGATTRSG
                     LISEPLKNRFGIQLRLEYYTDEEMQKIVERSAKLMNLEIEKEASFEIGKRSRKTPRIA
                     NHLLKRVRDFADVQNEGRIDLAICRSALEKLGIDSLGLDSIDRQILECIVDRYRGGPV
                     GLKPIAVVIGEEEKTVEETYEPYLVRIGLINRTPSGRTASEKAYEHLGRKRENQERTL
                     FS"
     gene            complement(1097..1537)
                     /locus_tag="PK683_07735"
     CDS             complement(1097..1537)
                     /locus_tag="PK683_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3805 domain-containing protein"
                     /protein_id="HNH08377.1"
                     /translation="MAFELDYQYFKSPEGWYSFVFPDYWETEVIEGVPVFYDPQGDGA
                     LVISAFKHLTGSYDLDEEMKRFLGQHSVEYDRNCIAVFENREGSRIQACEFYSKERFW
                     LVYMIAHRNKLIIASYNSDERPDADLSQILTRIISSIHFIVEPK"
     gene            complement(1638..>2493)
                     /gene="recA"
                     /locus_tag="PK683_07740"
     CDS             complement(1638..>2493)
                     /gene="recA"
                     /locus_tag="PK683_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004766281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNH08378.1"
                     /translation="KQGGVAAFIDAEHALDPSYAKKLGVNIDDLLVSQPDNGEEALEI
                     CESLVRSNAIDMIVIDSVAALVPKAEIEGDMGDSHMGLQARLMSQALRKLTGTISKSK
                     TTVVFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRKIETLKDKDDPIGNRVRV
                     KIVKNKCAPPFKQAEFDVMFASGINRESSLIDLGVKHELLVKGGSWYSYNNEKIGQGK
                     ESARNFLLENPEIAKKIEAQIRNLNDLPEKYSLDGSVNQISENAPQKPEKSSSRKKSA
                     LEEELDEV"
BASE COUNT          593 a          596 c          511 g          793 t
ORIGIN      
        1 agagatgtga gataatgaat gcaaggataa ggaggacagt gcctgctgaa aagaacattc
       61 ttctttcatc ttcactgagt ttttccagtt cagctgaaaa gggttctttc ctgattttct
      121 ctttttcttc cgagatgttc atatgctttt tctgacgctg ttcttcctga aggtgttctg
      181 ttaatcagac cgattctcac taaataaggt tcgtaggttt cttcaactgt tttttcttcc
      241 tcaccgatga caactgcaat aggcttcagt cccactggcc cgcctctgta cctgtccaca
      301 atgcattcca gaatctgcct gtcaatcgag tctagtccca gactgtctat ccccagtttt
      361 tcgagtgctg atctgcagat ggccaaatct atcctgcctt cattctgcac atcggcaaag
      421 tctctcaccc tcttcagaag atgatttgca atcctcggag tcttcctgct ccttttcccg
      481 atttcaaaag aggcttcctt ttcaatctca aggttcatca gttttgcaga tctttccaca
      541 atcttctgca tctcttcatc tgtatagtat tcgagtctga gctgtattcc gaacctgttt
      601 ttcaaaggct cgctgatgag tccgcttctt gtggtagctc caacaagagt gaaaggctga
      661 agagggatct gcaccgactg tgccgtaatt ccttcaccga tgactatgtc tatctggaag
      721 tcttccatag ccggatagag aatttcttca agacctcttc ccaggctgtg gatctcatca
      781 ataaacagta tgtccttgtc ggaaagcacc gtgagaaacc gtgcaaggtc tgctcccttg
      841 gatatgacct gtgcagatgt cactgtcaga ttggaaccga tttcctttga gataatgttt
      901 gcaagagttg tctttcccag tcccggcgga cctgaaagaa gaacatgatc gagtgccttt
      961 cctctcttcc ttgcagcatg aataaagacc ttcagatttt ccaggatttc tttctgtccg
     1021 atgaattcat cgagagagga gggacgcagg aatatatctt cttcagcctt gttttcaggg
     1081 ttgaccagtc tttctttcat ttaggttcca caatgaaatg gatggaagaa atgattcgag
     1141 tgagaatctg cgaaagatcg gcatccggtc tttcatcact gttgtaagat gcaatgatca
     1201 gtttatttct gtgagcaatc atatacacta accagaatct ttctttggaa tagaattcac
     1261 aggcctgaat cctgcttcct tctctgttct caaagaccgc aatgcagttt ctgtcgtact
     1321 cgacactgtg ctgtcctaaa aaccgtttca tttcctcatc gagatcataa gagccggtga
     1381 ggtgcttgaa ggcggaaatg acgagtgcac cgtcgccttg cgggtcataa aacacaggta
     1441 caccttcgat gacttccgtt tcccagtaat cgggaaacac gaagctgtac catccttcgg
     1501 gagatttaaa gtattgataa tcgagttcaa atgccatgat aaaaaaaaac cggatctttc
     1561 gatccggctc aatagttcct aaaaagaaag ccattaattg aaaaaaactt gtctacaggg
     1621 acaattttac attaccctta tacttcgtcc agttcttctt ccagtgccga tttttttctt
     1681 gatgaacttt tttccggctt ttgcggcgca ttttcagaaa tttgattgac agaaccgtcc
     1741 agactgtatt tttccggcaa atcgttcaga ttccgaatct gagcttcaat tttctttgca
     1801 atttcaggat tttccagaag gaagtttctg gctgattcct tgccctgtcc aatcttttcg
     1861 ttgttgtaag aataccagga cccgcctttg accagaagtt catgttttac cccaagatca
     1921 atgagtgaac tttctctgtt gattcctgaa gcgaacatca catcaaattc agcctgcttg
     1981 aagggaggcg cacatttgtt tttgacaatc tttaccctca ccctgttccc gatcggatca
     2041 tccttgtcct tcagtgtttc gatctttctg atgtcgagtc ttacgctggc atagaatttg
     2101 agtgcatttc cccctgtggt cgtttcagga gaaccgaaca tcacgccaat tttcattctg
     2161 atctggttga tgaaaactac tgtggtcttg gatttggaaa ttgttccggt gagttttctg
     2221 agtgcctgtg acatcagtct tgcctgaagc cccatatgcg aatctcccat atctccttcg
     2281 atctctgctt tcggcacaag tgcagcaacc gaatcgatta cgatcatatc aattgcattg
     2341 cttctgacca gggactcgca gatttcgagt gcttcctctc cgttgtccgg ctgagatacc
     2401 aggaggtcat cgatgtttac tcccagtttt ttggcataag aagggtcgag ggcatgttct
     2461 gcatcgatga atgctgctac gcctccctgc ttt
//
LOCUS       DAOQGJ010000421         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26839_length_2491_cov_3.220033, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000421 DAOQGJ010000000
VERSION     DAOQGJ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26839_length_2491_cov_3.220033"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PK683_07745"
     CDS             <1..239
                     /locus_tag="PK683_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNH08379.1"
                     /translation="EQMISSTEKFGILDESYLDSGVAGYILNRISGKYLAKFTKTFAL
                     PPEVIPHGERDEILKSYRLSPELLAEEILKLFYD"
     gene            complement(230..490)
                     /locus_tag="PK683_07750"
     CDS             complement(230..490)
                     /locus_tag="PK683_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08380.1"
                     /translation="MENITEKINELKKLISEFRKSVPELKATEIGIYRKEISGQIKSL
                     ESVSSELKEIQSDLNKVDSGKSDRPNSEKYVLAPDIEDPFQS"
     gene            964..1383
                     /locus_tag="PK683_07755"
     CDS             964..1383
                     /locus_tag="PK683_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08381.1"
                     /translation="MNRYSMIKNGFYLAGAANILGVLIFSLGFTNRHISSVYPEVMSV
                     FGLVMIIVWGLVFIAAGYSENFRYLSAVFAIEKTAYVISWFMWILRPEANLSAVFHQS
                     VLSGIFYAVYGANDLLFGIFFIWVFITLSKEISGLTA"
     gene            1813..>2491
                     /locus_tag="PK683_07760"
     CDS             1813..>2491
                     /locus_tag="PK683_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH08382.1"
                     /translation="MNRPEKSNSISRELLQNLNSAVLSFKNDPEIRCLMITSEGGKTF
                     CAGADLEERSRMSREEVFQFLNDFRNLLHLLENLEIPTVSAMNGSAFGGGLELALATD
                     IRLVKEDSVIGLTETKLGIIPGAGGTQRLARLIGKASAKDLIFRAARINSAEALRLGI
                     VQRVIIDNFIDLSVEYCREICSSAPIAVRAAKKAVERGFSEDIERGLDIERECYLLTL
                     DTEDRNEA"
BASE COUNT          714 a          447 c          541 g          789 t
ORIGIN      
        1 ttgaacagat gatcagcagt acagagaaat tcggcatact tgatgagagt tatctggatt
       61 caggagtggc aggatatatt ttaaacagaa tatcaggaaa atatcttgca aagttcacaa
      121 agacttttgc tcttcctccg gaagtgattc ctcacggaga aagagatgag attcttaaga
      181 gttacagact ttcacctgaa cttctggctg aggagattct gaagcttttt tatgactgaa
      241 aaggatcttc aatatccgga gccagaacgt atttttccga attgggtctg tccgatttcc
      301 ctgagtcaac tttattcaga tctgactgga tttctttgag ttcggaagaa actgattcaa
      361 gtgatttgat ctgcccggat atttcttttc tgtaaatccc gatctcagtc gctttcagtt
      421 cgggaacaga ttttctgaat tccgatatga gtttcttcag ttcattgatt ttttcagtga
      481 tattttccat agctgcggat gagtatagat atgctgataa aattgtaaag tttttattgt
      541 caattctttt atatttctcc cgtttccaaa aaagtctgct gtattcaaaa tattcaatgg
      601 aaaatcctga ttttcaggta tttccctgta ttcctgacag cttttttatg caggcggata
      661 tggaaaatgc ctcaaaaatt ttctttaact gatttttaaa acagaactgt cttgacttta
      721 ttaatcatac aggtaagctt ttctccatga aacaaattat tcccttacag ctctttcttt
      781 tttttctgaa cgaatgtgca gtaagttctt ccggtaagtc caagtcttct ttaaaatcag
      841 tcagaagagt ccctataggc acatctgcag tcagattatt taagttgatc agttcagttt
      901 ctttcaaaaa ttgtattatg cggcagtggt ttttcttggc agcctttcgg aagcaggttt
      961 aaaatgaaca gatatagtat gataaaaaac ggattttatc tggcaggggc ggcaaatatc
     1021 cttggggtgc ttattttttc tctcggattt acaaacagac atatttccag tgtgtatccg
     1081 gaggtaatgt cagtattcgg tctggtgatg attattgtat ggggcctagt ctttatagcc
     1141 gcaggatatt cggagaattt cagatatctg tctgccgtat ttgccattga gaagactgcc
     1201 tatgtgattt cctggtttat gtggatactt cggcctgagg cgaatctgtc tgcagttttt
     1261 catcagtcag ttctttccgg aattttctat gcagtttatg gagccaacga tcttttattt
     1321 gggatatttt ttatatgggt tttcattact ctttcaaagg agatatccgg actgactgca
     1381 taactgaaca gtcgcgggct ttgtaaaaaa taaatttcac agtgaagggc acgaagcgaa
     1441 aaaaaaatgg gaggtctgag gcacattgta tttgctggct gactcacgtt tttgttttta
     1501 gacaaaatcc aattgacgga tttaaaagaa aaatatggac tttcaaaaaa aatgatatga
     1561 ttgataatca acccttaggg acgccgccca tacgccccta agggttgacg cggaattgta
     1621 tgaggctttt ttaggcaaaa attctttttc aaaaaataat actgcaattg taaaaatgca
     1681 ggcagaggct tcctcctctg tcttgctgag aatcatagtt taattactga tagactgcag
     1741 cacctcatat ttttaaattg tctcatgtcg gcagtaattt ccgaaaaaaa agagaacatc
     1801 gggatcatcc gcttgaaccg tcccgaaaaa agcaattcta tctccagaga actgcttcaa
     1861 aacctgaaca gtgcagttct ttcattcaaa aatgatcctg aaatccggtg tctgatgatt
     1921 acatctgagg gcgggaaaac attctgtgcg ggtgccgatc tcgaagaaag aagcaggatg
     1981 tccagagaag aagtttttca gtttttgaat gatttcagaa atctgcttca tcttctggaa
     2041 aatctggaga ttcccactgt ttctgcaatg aacggctctg cttttggagg cgggctggaa
     2101 cttgcacttg cgacagatat ccgtctggtg aaggaagatt ctgtgatagg tctgactgaa
     2161 acgaaactgg gaatcattcc cggtgccggg gggactcaga gacttgcaag actgattgga
     2221 aaagcatccg caaaagatct gattttccgg gcagctcgaa taaattctgc agaagcactg
     2281 agactcggaa ttgtacagag agttattata gataatttta tagatttatc cgtcgaatac
     2341 tgcagagaga tctgcagttc ggcgccgatt gcagtccgtg cagcaaaaaa agcagtggaa
     2401 agggggtttt ctgaggatat tgaaagagga ctggatatcg aaagagaatg ttaccttctc
     2461 actcttgaca cagaagacag aaatgaggcg c
//
LOCUS       DAOQGJ010000422         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26842_length_2491_cov_2.482348, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000422 DAOQGJ010000000
VERSION     DAOQGJ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26842_length_2491_cov_2.482348"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK683_07765"
     CDS             complement(<1..556)
                     /locus_tag="PK683_07765"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH08383.1"
                     /translation="MNLNAGHSFNEKQKLNIFNMILEAFPFNLFVKNTESRYVITSSV
                     CDKENGVERGALIGKNDYDLQSSRELAEIFINDDRRIIQSKKGSRMLTPVTCGSQITY
                     YDIYKEPLIDKFGKVYGVLGLVMNLEDSRRSMSLERIKGNIENGKSDGICIMFDFDLD
                     TGYAYVFRNTSEIRLKITSDRTFRE"
     gene            complement(1101..1502)
                     /locus_tag="PK683_07770"
     CDS             complement(1101..1502)
                     /locus_tag="PK683_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08384.1"
                     /translation="MKEIKNEYVDQIYDTELKVLYANFHKFGPFSEYLPIFENGYEII
                     QMQKAKGCLINIERVTVHDEQTRAYLGEVWFPKVSQEGVTHIAIVLPSSALGRISSQK
                     AHESAEIIGSLTICNFDRETEAREWLSKMLR"
     gene            1948..>2491
                     /locus_tag="PK683_07775"
     CDS             1948..>2491
                     /locus_tag="PK683_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNH08385.1"
                     /translation="MTMRMKMTALVAVGLIGILVIAVVSQFQMNSVYRKANYANENTV
                     PSLALIAESGSLVEIMRVTTWQYFSQKDPFKRRALGEKISGLRLKVDESLKKYEGFLS
                     NARDKELLSEDRTFLLDYDRLREKVLLLSNADRNEEARDLLFANQETMTKISDAFGRH
                     REFNIQLAAASAAEAVEIRQS"
BASE COUNT          718 a          417 c          486 g          870 t
ORIGIN      
        1 gttccctaaa ggttctgtct gaggtgattt ttagacggat ttctgaagta tttctgaaaa
       61 cataagcata ccctgtatcc agatcaaagt caaacataat gcagatgccg tctgatttac
      121 cgttttcaat gtttcctttt atcctttcca gggacatgct tcttctggaa tcttcaagat
      181 tcataacgag gccaagaact ccataaactt ttccaaactt gtcaatcagg ggctctttat
      241 atatgtcgta ataagtaatc tgtgatccgc aggtcactgg tgtcagcatt ctgcttcctt
      301 ttttagattg aattattcta cggtcgtcat ttatgaatat ttctgccagt tccctggatg
      361 actgcagatc ataatcattt ttaccaatca gagcccctct ctcaactcca ttttccttgt
      421 cacacacact gctggtgatg acatacctgc tttcggtatt ttttacaaac agattgaaag
      481 gaaaagcttc aagaatcata ttaaaaatat tcagtttctg tttttcattg aaactgtgtc
      541 ctgcgttcaa gttcatccaa gctcctgact ttgctgtatt acgaattgct tacaacggat
      601 ttaatcaagt ggatgatgtc aattgaaaat taaagcgtac tgcttgacct ctgtctcatt
      661 ttgattatgc cagtgggcgc agagtcaata aaatcgggag tgttgtatgt ctgaacactg
      721 gatttcaatt cacaaaacaa ttattttgca ggaataatta aatgagttaa ttataaatac
      781 ttaaataata aattatgtat ccattctctt cacgagttca ttttgagcag ttgagtgcaa
      841 tgacgtttaa taaataatat cgccttctcg aaagggtcgg ttttgctgag ttgtaaaatt
      901 tggttttcac cgagaagtgc cgcagttttg gatgcttcct ggaacagcgg cttcccagtt
      961 tacggagcaa atgctgagtt ttaattataa gttatagttt tatgattaaa tgagttaatt
     1021 ataaaactat ttttaatctc attctaaaat gatcctgatt cagtcagaat ttctgcattt
     1081 tacttataca gggtcagttt tcatctcagc atttttgaca gccattcgcg tgcttcagtt
     1141 tcacggtcaa aattgcagat ggtcaaactg cctatgattt ctgcgctttc atgtgccttt
     1201 tgtgaagata ttcttccaag tgcagaactt ggaaggacaa ttgctatatg tgtaacacct
     1261 tcctgactta ctttgggaaa ccatacttct ccaagatatg cacgagtctg ttcatcgtgt
     1321 actgtgacgc gctcaatgtt gattagacag ccctttgctt tttgcatttg tataatctca
     1381 taaccgtttt caaagattgg gaggtattca ctgaatggtc caaatttgtg gaaatttgcg
     1441 tacagtactt tcagttcagt atcatagatc tggtcaacat attcgttctt aatttctttc
     1501 atttttatcg catagtatga agcttaagaa aagtaaacaa atttttattt tattcgtatt
     1561 ctgtgctttt ttataataat tgttattttt tatattctga ataataaaat gatttctttt
     1621 tgcgagagaa gtttttcgat gatggaaatc tccaattaat atgtttaatt ataatccgtg
     1681 ttgattgtga ttctatgttt catcatgaaa taaattttat cgtatgtcag ggttttattt
     1741 gtaaattgat tcctgtttaa tatattcttg aaaaaaaagg gcaatgaaaa cttaacgtac
     1801 attatacatt cttgagaaat gactatgtgt ggccggaaat tattgggttt aattatgctg
     1861 ttccataatc taatgaaaga atatattata tattattgaa agatatacgg tgtttgatga
     1921 tttcttgcag atgtcgggag gatttttatg actatgagga tgaaaatgac tgcattggtt
     1981 gcagttgggt taatcggtat tttggtgatt gcagtcgtat ctcagtttca gatgaacagt
     2041 gtttatagga aagcgaatta tgcaaatgag aatactgtac ccagtctggc tttgattgct
     2101 gaatcaggtt ccttagttga aattatgcga gtaacaacat ggcaatattt ttctcagaag
     2161 gaccctttca agagaagggc attgggagag aaaataagcg gactgagact gaaggttgat
     2221 gaaagcctga agaaatatga aggttttctt tccaatgcaa gggataaaga acttctttct
     2281 gaagacagga cgtttcttct tgactacgac cggctgaggg aaaaagtatt gctgctctcg
     2341 aatgcggacc gaaatgaaga agcccgtgat ctgctgtttg cgaatcagga aacaatgaca
     2401 aaaatttccg atgcttttgg cagacaccgt gagttcaata ttcagctggc agctgcatct
     2461 gctgcggaag cagttgaaat caggcagagt g
//
LOCUS       DAOQGJ010000423         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26926_length_2486_cov_3.004525, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000423 DAOQGJ010000000
VERSION     DAOQGJ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26926_length_2486_cov_3.004525"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PK683_07780"
     CDS             complement(<1..269)
                     /locus_tag="PK683_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08386.1"
                     /translation="MLILFILSFTEVSPKELRKNRLTEKKSADANFENEDLLMELSIS
                     ELQREILQEKLDVLNSEIIELNPLLDPNSDPEKKKIRFRQNIIFPG"
     gene            complement(464..1495)
                     /locus_tag="PK683_07785"
     CDS             complement(464..1495)
                     /locus_tag="PK683_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08387.1"
                     /translation="MFDSVILYFKSKFSKSKNEHSSVIYRSPEEWEGVWGNLQKEKEN
                     YSPISLNEYLSKNGHREAQFKELLEKEFQKDSSVKKQNRTPAYFGLKPALASVIILIF
                     GILLYTFYSSQEKTALKYLPLKGSVRLERNGQIIQIREKEPFEKNDRLILKDEESLEI
                     VYSDSLKFRLEGSADMEIREIPESENKIHFLIHLGTIEIFSNKPKNRPEIAWITDRFT
                     YIPTGTVARLKVGSTAEELTVSQGSFICKDRIGKVRQIKESESFKFSEADLDLDQESN
                     DIEGAKKNAVPNPPRKEDQEIFGIVQTVRLKNGEKHTGIYYKKNGKTYLNVPGRTMEL
                     SDSEIESVE"
     gene            complement(1488..2009)
                     /locus_tag="PK683_07790"
     CDS             complement(1488..2009)
                     /locus_tag="PK683_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH08388.1"
                     /translation="MENSTDFKELETIYEQNKDFLFSKFLYLTGSSDEAYDLIQAVFL
                     KLIPYFRQGKIKSVNKSFLYKVGYNIFSEDFNKRKNRNSRDIQYFMIRNGENREEPET
                     DNILEIVNSEIENLDVSERMKISLRMRLYAENSLQEIAEASGVSVRTVIRDLNAGLER
                     LKEKLKKKGIGHV"
     gene            complement(2049..2393)
                     /locus_tag="PK683_07795"
     CDS             complement(2049..2393)
                     /locus_tag="PK683_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08389.1"
                     /translation="MVKYFLFIVLFPNISYADRIFLTNDDRLSGEIWKQTPQTAIISC
                     EHGNYKLISRNIRSVEYAGTNRYRLMLNTGESTVIFPVKSESGELIYRKENDLLISAQ
                     WSKIVFLRLIKK"
BASE COUNT          631 a          541 c          429 g          885 t
ORIGIN      
        1 cccgggaaaa ttatattctg acggaatctt attttctttt tttccggatc tgagtttgga
       61 tccaaaagag gattcagttc aataatttca gaattcagta catccagctt ttcctgaaga
      121 atttccctct gaagctcgct gatgctcagc tccatcagaa gatcttcatt ttcaaaattt
      181 gcatctgctg attttttttc tgtgagtctg tttttcctta attccttcgg actgacttca
      241 gtaaaagaca gaatgaagag aattaacata actttcaggc attgacagac acaggacagt
      301 cggattgcct gtctgttttt atatgatatt ccagtcattt gaagaagata aaatatattc
      361 tcatgataat atacttaagt tacatacgag cagaaccgcg tgaatcggac tgtctgcagc
      421 aggtctgtca tattgcgaag atcgacattc tagtcagtga aaactattcc actgactcaa
      481 tttcactgtc actcagttcc atcgttctgc ctggtacatt cagataagtt ttcccgtttt
      541 tcttataata gatccctgta tgtttttctc cgttcttgag tctgacagtc tgaacaattc
      601 caaaaatttc ctgatcttcc tttctcggag gattaggcac agcattcttt ttagccccct
      661 cgatatcgtt gctttcctga tccaggtcca gatccgcttc actgaattta aaactctcac
      721 tttctttgat ttgacgcact ttgcctattc tgtccttaca gataaagctt ccctgagata
      781 ctgtcagttc ttctgctgtg cttcctactt tcagtcttgc cacagtgccg gttggtatat
      841 atgtaaatct gtctgtaatc caagcaattt ccggtctgtt cttcggctta ttggagaaaa
      901 tttcaatcgt tcccaaatga atgagaaaat gaattttgtt ttctgactca ggaatttccc
      961 tgatttccat atctgcagat ccttccagtc tgaatttcag agaatcactg tagacaattt
     1021 ccagagattc ctcatccttc agaatcagtc tgtcattttt ctcaaacggt tctttttccc
     1081 gtatttgaat gatctgtccg tttctctcca gacgcactga tccttttaaa ggaagatatt
     1141 tcagtgcagt tttttcctgg ctgctgtaga atgtataaag gagtattcca aaaatcagaa
     1201 tgatgactga agccaatgcc ggcttcaggc caaagtaagc aggagtcctg ttctgttttt
     1261 ttactgaact gtctttttga aactcctttt ccagaagttc cttgaactgt gcttccctgt
     1321 gtccgttttt ggaaagatat tcattcagtg agattggact gtaattttcc ttttccttct
     1381 gaagatttcc ccagacgcct tcccattcct ccggggatct gtatatgact gaagaatgct
     1441 cattcttcga ctttgaaaac tttgatttga aatacagaat gactgaatca aacatgcccg
     1501 attccttttt tctttagttt ttccttcagt ctttccagac ctgcattcag atcccgaatg
     1561 actgtcctga ctgatacacc tgaggcttct gcaatttcct gaagactgtt ttctgcatac
     1621 agcctcattc ttagactgat cttcatccgt tcacttacat ccagattttc tatctctgaa
     1681 ttgactattt ccagaatatt gtcagtttcc ggctcctctc tgttctctcc gttccgtatc
     1741 ataaaatact gtatgtctct gctgtttctg ttctttctct tattgaagtc ttccgagaat
     1801 atattgtatc cgactttgta taaaaagctt ttgttcacac tttttatctt cccctgcctg
     1861 aagtagggaa taagtttcag aaacactgcc tgaataagat cgtacgcctc atcagaactg
     1921 cctgtcaaat aaagaaattt actgaataga aagtctttgt tctgctcata gattgtctcc
     1981 agttccttaa agtctgtgct gttctccata attaaccgtc ctgactgccg gagtatgaca
     2041 taaaagaatt atttttttat cagtctgagg aaaacaattt tcgaccattg tgcagaaatg
     2101 agcagatcat tctctttcct gtaaataagt tctcctgatt ctgatttgac tggaaaaata
     2161 acggtactct cgcccgtatt cagcataagt ctgtatctgt ttgtccctgc atattcgaca
     2221 cttcggatgt ttctggatat gagtttgtag tttccgtgtt cacagctgat gatggcagtc
     2281 tgcggggtct gcttccatat ttctccgctc agtctgtcgt catttgtcaa aaagatacgg
     2341 tccgcatagg aaatattcgg gaatagaaca atgaacagga aatactttac cattctgatt
     2401 ttgagtcctt aaactgacca atacttcatt ttccgggtat ttcatattta aggtcaatta
     2461 aaattaaaaa ttagaatgga tcattt
//
LOCUS       DAOQGJ010000424         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_26946_length_2485_cov_2.956379, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000424 DAOQGJ010000000
VERSION     DAOQGJ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26946_length_2485_cov_2.956379"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PK683_07800"
     CDS             complement(<1..200)
                     /locus_tag="PK683_07800"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNH08390.1"
                     /translation="MKIYTKTGDRGDTSLADGKRVRKSDPRVELYGTVDELNSFIGGA
                     LSFIGHDFSEKKTLTEIQNILF"
     gene            complement(197..>2485)
                     /locus_tag="PK683_07805"
     CDS             complement(197..>2485)
                     /locus_tag="PK683_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNH08391.1"
                     /translation="TVFFVHRKPMAVFLRIFFFFFLFFPSLLLAEEDIQYDIKTMSWR
                     KINRNLSSKTSITQTEAFALVRYHEEVKNPDKEFRFRLLYGIASGRYPERIGPPEVET
                     LLAEKFSFGKVIFRISYWKLYQELSRRKLLTVKQKLRYLEKAPLENDPVFLRSWEEQL
                     SVLASNGYYDAVIEKMKNVEKEKPFLFTGHSKYLLAHSLWKDGKGSKALSVYYRLLSS
                     DADPEWKKMAVKDLRRVIQEDFYMKLEPLELSSVIPYLSGKEKKNLKSRGAVNSSLGD
                     KTAAMNIALFYAGYMPGQILPFLSKNPELAKDDSLIGRLADELISGKYYDAAEELIGK
                     YSSSSDPGIQKQKARLYKKKGSKEKFFNATVSYLTVFPYDLTYQDYLIDFLVSTGANS
                     IHYAKENYWETAFSKIPNVPVKGRLVYWYLRYLKFRQNKTKFNEVLERAYEHCPGSYY
                     NVVINEEFHQEISLLTVPSSPFASRESLLKYFSLKKYDEYSSVLTGHNLSFAYFKDSQ
                     ELAGRLKTAREKVKNSQYLQTAAEYLRYGHMKEALEMADAYSREQNLSLNQKYEIYVG
                     LGDSGRNAYMSLFYTRQLMKNYMIPDDPLLLPEDLSSRLYPRPHRDLVVKNASEYNVE
                     EEVVYAIMRQESFFRENAVSPSNARGLMQVMPATGKFLAGKLGVKDYSLHDPEYSIRF
                     GAKFLGDLLRNYEGKLTWAAIAYNGGPGNLRKWRRNHYKNDFNHFLEELPSKESRDYC
                     RIIISNYRNYKVLSNLQRYYPL"
BASE COUNT          675 a          555 c          510 g          745 t
ORIGIN      
        1 tcgaacagaa tattctgtat ctcagtcaga gtttttttct cactgaaatc atgaccgatg
       61 aaactgagag ctcccccgat aaaactgttc agctcgtcga ctgtgccgta aagttccact
      121 cttggatccg actttctgac tcgttttccg tccgcaaggg aagtatcacc tctgtcgcct
      181 gtcttagtgt aaattttcat aaaggatagt acctctggag attgctgagc accttgtagt
      241 ttctgtaatt ggagataatg atcctgcagt agtcccttga ttccttggaa ggaagctctt
      301 ccagaaaatg attgaagtca ttcttataat gatttcgtct ccatttccga agatttcccg
      361 ggcctccatt gtatgcaatt gcagcccagg tcagcttgcc ttcatagttt cggagaagat
      421 ccccaagaaa ttttgcaccg aaccggatgg aatattccgg atcgtgaaga gaatagtctt
      481 ttacaccgag tttacctgcc aaaaatttcc cggtggcagg cataacctgc atcagtcctc
      541 ttgcattgga gggagagact gcattctctc tgaaaaaaga ttcctgtctc attatggcgt
      601 atacaacttc ctcttccaca ttgtattcgg aagcattctt gacgaccaga tccctgtgag
      661 gtctgggata cagtctggag gaaaggtctt caggaagaag aagaggatca tcaggaatca
      721 tataattttt catcagctgt ctggtataaa agagtgacat atatgcattt ctgccggaat
      781 cacccagacc gacgtaaatt tcatacttct gattcagaga aagattctgt tccctggaat
      841 atgcatcagc catctccagg gcctctttca tatgcccata ccgaaggtac tcggctgccg
      901 tctgaagata ctgactgttt tttacttttt ctctggctgt cttaagccgg ccagccagtt
      961 cctgtgaatc cttgaaatag gcaaaactga gattatgccc ggtcagaaca gaagaatatt
     1021 catcatattt ttttaatgaa aaatatttca aaagactttc tctggatgcg aacggagagg
     1081 aaggaacagt cagaagtgaa atttcctgat gaaactcttc attgatgaca acattgtagt
     1141 aggatccggg gcagtgttca taagcccttt caagcacttc attgaacttt gtcttgttct
     1201 gtctgaactt cagatatctg agataccagt atacaagtct tcctttgaca ggaacattcg
     1261 gtattttgct gaatgcagtt tcccagtaat tttcctttgc ataatgaatt gaattcgcac
     1321 cggtgctgac aaggaaatca atcagataat cctgatatgt aagatcataa ggaaatactg
     1381 taagataact tacagttgca ttgaaaaatt tctccttgct tccctttttc ttgtacagtc
     1441 ttgctttctg cttctgaatt cccggatcag aacttgagga atactttccg atgagctctt
     1501 ctgccgcatc atagtatttc cctgaaatca gctcatctgc cagtctgccg atcagagaat
     1561 catcttttgc cagttcggga ttcttggaaa gaaacggcag aatctgtccg ggcatgtacc
     1621 ctgcgtaaaa aagtgcaata ttcattgcag cagtcttgtc tccaagagat gaattcactg
     1681 ctcccctgct cttcagattt ttcttttcct tgccggaaag ataaggtatt accgatgaaa
     1741 gctcgagagg ttcaagcttc atataaaaat cttcctgaat taccctcctg agatctttga
     1801 cagccatttt tttccactca gggtctgcat ccgaagacag cagcctgtaa tagacggaaa
     1861 gagcctttga tcccttgccg tccttccata aggagtgcgc aagaagatac tttgaatgac
     1921 ctgtgaacag aaacggcttc tccttttcca catttttcat tttttcaatg accgcatcat
     1981 agtatccgtt tgaagccaag acggacagct gctcctccca ggaccgaaga aacacagggt
     2041 cattttccaa aggagctttt tccaggtatc tgagcttctg tttcacagtc aggagtttcc
     2101 ttctgctcag ttcctgataa agtttccagt aggaaatcct gaagatcacc tttccgaagc
     2161 tgaacttttc agccagaagg gtttccacct caggcgggcc tatcctctcg ggatatcttc
     2221 cggaagcaat tccgtataaa agacggaatc tgaattcctt gtcagggttt ttgacttctt
     2281 catggtacct tacgagcgca aaggcttccg tctgggtgat ggatgttttg gaagataaat
     2341 ttctgttgat ttttctccag ctcatcgttt tgatatcata ctggatatct tcctctgcca
     2401 aaagcaggga gggaaaaaac aaaaaaaaga agaaaaagat tcggagaaaa acagccatag
     2461 gttttctatg gacaaaaaaa acggt
//
LOCUS       DAOQGJ010000425         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27034_length_2479_cov_4.134488, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000425 DAOQGJ010000000
VERSION     DAOQGJ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27034_length_2479_cov_4.134488"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..386)
                     /locus_tag="PK683_07810"
     CDS             complement(6..386)
                     /locus_tag="PK683_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08392.1"
                     /translation="MYKNAVIIIMFLILGCTSRMGDFTVVSTRNSNIKNWKRHSERVE
                     GSSCKWYLFFIKVKDWDLKDAIEDAVDKTNRSSSSKNESLLDVKLSTNWFHLILFGRS
                     CIVAEGTPADSWYVTEEREEQIKK"
     gene            539..1846
                     /locus_tag="PK683_07815"
     CDS             539..1846
                     /locus_tag="PK683_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08393.1"
                     /translation="MNRKDKAVSRINSAKTGQKTLQETEDQMKNPRHWFEFYGEKLHK
                     FWKDKKAEGRTLEGLYLLMGKLSDGHTDNAISTIKNKFIREKSLTKELIPAAAVLLLE
                     TDFYSQFFDIGMLHRLDKQNTEKQSRTSYDLLKIKSADKTDIIWNRWLNLFREKYIEP
                     ENTEKFYSVESGKTDSKEIIDLMERLRKEESDLYEIINFRKDVIDRFYKLSVIFHYRS
                     FLHSELFRKDIENNDRKRKGPEIDLLLNEADRLLDDLEKYSSRPEFGEFYEDFRLYRN
                     YQTGNHMFGTAVFKDLSQKEQKTELERKKKYMEIMLPREDDSHTYSIWYNYYYFHYLQ
                     NRMDYFMNSGSLEKGLEECEKCIQLLKALDGKLKGGFLRLSLKIKYNLRILYLIYYSV
                     EAMIQERSVGLSIDGTEYEKEELSEKRDRTVMDILEWLRSIKK"
     gene            2014..2364
                     /locus_tag="PK683_07820"
     CDS             2014..2364
                     /locus_tag="PK683_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08394.1"
                     /translation="MKVLSFLFLPIFFGFCRLDAKDNKSKTNVIPSALHGFYCEEGFP
                     KGADCIPLIIEANSVFDSRMGGCNKIKDVIQKEAVYTVSCLEGESVSIKILSKDKILL
                     KVGNFQEIRERHKQ"
BASE COUNT          849 a          450 c          475 g          705 t
ORIGIN      
        1 tgttttcatt tcttaatctg ctcctctctc tcttcagtca cataccagct gtccgccggc
       61 gttccttctg ccacaatgca ggatctgccg aacaggatca gatgaaacca gttagtagac
      121 agcttcacat ccagaagaga ttcattctta gagctggagg atctgtttgt cttatcaacc
      181 gcgtcttcta ttgcatcttt caaatcccag tctttcactt ttataaagaa caaataccac
      241 ttgcagctgc tcccttccac tctttcagaa tgtcttttcc agtttttgat attggaattt
      301 ctcgtggaaa ctactgtaaa atctcccatc ctggatgtac atcccagaat cagaaacata
      361 attataataa ctgcattttt atacataaat tctcctttaa tatgcagaca tattttttgc
      421 acacaaattc acagaatacc gaataaaaat acagacaaga caaaaaatgc gatttacagt
      481 cagataaaag cttattttgg tgtataaaaa gtacaccaga tttatgggaa aacatcagat
      541 gaacagaaaa gataaagctg tttccagaat caattcagcc aagactggac agaaaactct
      601 gcaggaaaca gaagatcaga tgaaaaatcc aaggcattgg tttgagtttt acggagaaaa
      661 acttcataaa ttctggaaag ataaaaaggc ggaaggaaga actctggaag gcctttatct
      721 cctgatggga aagctgagcg acgggcatac cgacaatgca atatctacaa taaagaataa
      781 gtttataaga gaaaaatcat taaccaaaga actgattcca gcggctgctg tccttctgct
      841 ggaaacagat ttttacagtc agttttttga cataggaatg ctccatcgac tggataagca
      901 gaatacagaa aaacagagca gaacatctta tgatctgtta aaaattaaaa gtgctgataa
      961 aactgatatt atctggaaca ggtggctgaa tctttttcgg gaaaaatata tagaacctga
     1021 aaacacggaa aagttttact cggttgaatc gggtaaaaca gattctaaag aaatcattga
     1081 tctgatggaa agactgcgaa aagaagaatc tgatttatat gagataatca atttcagaaa
     1141 agatgtcata gacagatttt ataaattatc tgtaatattt cattatagat catttctcca
     1201 ctccgagctg ttcagaaaag atatcgagaa taatgataga aagagaaaag gaccagaaat
     1261 tgacctcctg ctgaacgaag cagatcggct tctggatgat cttgaaaaat attcttctcg
     1321 tcctgaattt ggagagttct atgaggactt cagactttac aggaattacc agacaggaaa
     1381 tcacatgttt ggtacagcag tcttcaagga tctcagtcag aaagaacaaa agacagaact
     1441 ggaaagaaag aaaaagtata tggaaattat gctgccaaga gaagatgaca gtcatacata
     1501 cagtatatgg tataattatt attattttca ctatcttcaa aaccgaatgg attattttat
     1561 gaattccggc agtcttgaaa aagggctgga agagtgtgaa aaatgcattc agcttctcaa
     1621 agctctggat ggaaaactga aaggaggatt cctgcgtctc agccttaaaa ttaaatacaa
     1681 tcttcggata ttatatctga tatattattc ggttgaggct atgattcagg aaaggagcgt
     1741 aggtctttct attgatggaa cagaatatga aaaagaggaa ttgtcagaaa aaagggacag
     1801 aaccgttatg gatattctgg aatggctcag atccataaaa aagtaattga gcagaatttt
     1861 catactttac ttttttaaac ctttcaaata tccctgtgca aaaaaactgt tgtcttttca
     1921 tactcggaaa tatgtgctga tggactgcct gcggactttt ctgaatatgt ctgcagtaag
     1981 ctctgactat tataaggata tgcaggttca acgatgaaag tactttcatt tctcttcctc
     2041 ccaatatttt ttggtttctg cagactggac gcaaaggaca ataaatcaaa gaccaatgtc
     2101 attccctccg cccttcatgg attttactgc gaggaaggat ttccgaaagg tgctgactgc
     2161 atccccctta tcattgaggc aaactcagtg tttgactcaa gaatgggcgg gtgcaacaag
     2221 attaaagatg tcatccaaaa ggaagccgtc tacactgtca gctgtctgga gggggaatcc
     2281 gtatcgataa agattctttc caaggacaaa atcctgctca aggttggaaa tttccaggaa
     2341 atcagggaac ggcataaaca gtaggatttg ccattcattc tcctgatact tgaagtcttt
     2401 actgttgaat acactgattc aaagaaaatt ttacctcgtt tagttcaaaa caacttttta
     2461 tgaaagactc gcaatcctt
//
LOCUS       DAOQGJ010000426         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27054_length_2478_cov_3.926537, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000426 DAOQGJ010000000
VERSION     DAOQGJ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27054_length_2478_cov_3.926537"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..372
                     /locus_tag="PK683_07825"
     CDS             124..372
                     /locus_tag="PK683_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HNH08395.1"
                     /translation="MSSKKILELKTPDLGDSEKIELVKWYVRKGESVQSGQEILELVT
                     DKAAFPMESPFDGTLEEILIPEGSTVQRHQVLGILSHL"
     gene            369..1238
                     /locus_tag="PK683_07830"
     CDS             369..1238
                     /locus_tag="PK683_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNH08396.1"
                     /translation="MKIIHISDLHFPVAISISRLGFSKRLIGYLNFVLRRKKLHPLEA
                     AEMMVSKIRNEQYDLLIISGDITNVGHDDEFAAARRILSPILNKKSFVIPGNHDRYVP
                     DSVSPADLFFRHFEEFAGTPVPGSIPDYLRTAKIGGITVVGMDSNSPRGMVDASGEIS
                     PSALKKYSDYIKSLNDPYILTAHHPVWNPPEKQESVYHKLLNREEFLTEIRKNPPMAY
                     FHGHVHTNWIRKKDSEIPFLISNAASSTRDTDRSHLCGFHILDLQKDSFSVQRISYSK
                     TKKSLSLKDPVVY"
     gene            complement(1242..2225)
                     /locus_tag="PK683_07835"
     CDS             complement(1242..2225)
                     /locus_tag="PK683_07835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH08397.1"
                     /translation="MKKRKLGKNGPEVSELGLGCMGMSEFYGERNDEESVAVIHRALE
                     LGVNFLDTADMYGTGDNEELLKKAIKDRRDKVFLATKFGNVRGKDGSFLGVNGKPEYV
                     RQACDASLKRLGVDHIDLYYQHRVDPETPIEDTVGAMVRLIEEGKVRYIGLSEAAPAT
                     VKKAEMVHHITALQTEYSLWTRDPETEILSVCEGLGIGFVAYSPLGRGFLTGAFQKAE
                     DIPNDYRKNSPRFQKENFESNLQIVENLKELAKEKNCTPPQLALAWVLAQGKYIVPIA
                     GTKKRKYLEENIQSAEIQLSLQDLERIDQLIPRGSAKGLRYPEQSMKSLNR"
BASE COUNT          693 a          564 c          469 g          752 t
ORIGIN      
        1 gatcttctct gtccttttat tctcatcctg cggacattcc gaaaacggcg cggagcagca
       61 aattttgttt cctgtaaaaa tatcttagat gagattcttg gcaagcagga aattatatat
      121 tttatgtcct cgaaaaaaat tttagaactt aaaacacctg atttaggtga cagcgaaaaa
      181 attgaactgg taaaatggta tgtcaggaaa ggggaatcag tccaatccgg tcaggagatt
      241 ctggaactgg tcacagacaa ggccgcattc cctatggaat cgccgtttga cggcacactg
      301 gaagaaattc tgatccctga aggctcaaca gtccaaagac accaggtgct tggcattcta
      361 agccatttat gaaaatcatt catatttccg acctgcactt tccagtcgca atttccatca
      421 gcagactggg attcagcaaa agactgatcg gatatctgaa ttttgtgctc cgcagaaaaa
      481 aactccatcc gctcgaagct gccgaaatga tggtgagcaa aatcagaaat gaacagtatg
      541 atcttttgat tatctccgga gacattacaa atgtcggtca cgatgatgaa tttgcggcag
      601 ccagacggat tctgtctcct attttgaata aaaaatcatt cgtcattcct ggaaaccatg
      661 acagatatgt tccggattcc gtctccccgg cagatctttt tttcagacat tttgaagagt
      721 ttgcaggaac tcctgtacca ggttccattc cggattatct cagaactgca aaaatagggg
      781 gaattactgt tgtcggaatg gattcaaatt cccctcgagg aatggtggat gcttcgggag
      841 aaatcagtcc ttctgcgctg aaaaagtatt ctgactatat aaaatcatta aatgatccat
      901 atatacttac agctcatcat cctgtctgga atcctcctga aaaacaggaa tctgtatatc
      961 ataagctttt gaacagggaa gagtttctga ccgagatcag aaaaaaccct cccatggctt
     1021 attttcacgg tcatgttcat acaaactgga tcagaaaaaa ggattcagaa attccattcc
     1081 tgatatcgaa tgcagcctca tcgaccagag acacagacag aagccatctg tgcggctttc
     1141 atattttgga tcttcaaaaa gacagttttt ctgttcagag aatttcctat tccaaaacaa
     1201 agaaaagtct ttcattaaaa gatccagtcg tgtactgacc tttatcgatt cagcgatttc
     1261 atcgactgtt ccggatatcg gagtcctttg gcagaacctc ttggaatcag ctgatcaatt
     1321 ctttccagat cttgcaggct gagctggatt tcagcactct gaatattttc ctccaggtat
     1381 tttctttttt tggttcctgc aatcggtacg atgtactttc cctgagccag aacccaggcg
     1441 agagcaagct gtggaggagt gcagtttttt tcctttgcca gttccttcag attttccact
     1501 atctgcagat tcgattcaaa attctctttt tggaatcggg gggaattctt cctgtaatca
     1561 ttaggaatgt cttccgcttt ctgaaaggct cctgtcaaaa agcctcttcc taaaggactg
     1621 tatgccacaa agccgatgcc cagtccttca cagacagaaa gaatttccgt ttcagggtcc
     1681 cttgtccaga gggaatactc tgtctgaaga gcagtgatat gatgaaccat ttccgccttt
     1741 tttactgttg caggagcggc ttcagaaaga ccgatataac ggacttttcc ttcttctatc
     1801 agacggacca ttgccccgac tgtatcctca atcggtgttt ccggatctac tctatgctga
     1861 taataaagat caatatgatc tactccaagc cttttaagac tcgcatcgca ggcctgtctg
     1921 acatattccg gttttccgtt tacacccagg aatgaaccgt ccttgcctct tacgtttccg
     1981 aatttggtgg caaggaaaac tttatcccgt ctgtctttta ttgctttttt caggagttct
     2041 tcattatcac ctgttccata catatcggct gtatccagaa aatttactcc cagttccagt
     2101 gctctgtgaa tgacagcgac agactcttca tcatttcttt caccgtaaaa ctcagacata
     2161 cccatacacc caagtccaag ctcagaaact tcaggaccgt ttttccccag ttttctcttt
     2221 tttatcattt gaattctcct tttattaaat tgtctttcta tactgaaact gtaaaagcct
     2281 gctccagacg ggataattga tttttatatg tccgtctcga ttgaaagatc cctatttttc
     2341 ctcaaggact acttccttca agaatagcga aataataagc gaaaatatta aaaagtatgc
     2401 agaaaccaga aatacatcgt tcagcactgc tcccatattt tctctcagaa catagatgga
     2461 ttctctgatt ttttccag
//
LOCUS       DAOQGJ010000427         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27088_length_2477_cov_3.317919, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000427 DAOQGJ010000000
VERSION     DAOQGJ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27088_length_2477_cov_3.317919"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2081
                     /locus_tag="PK683_07840"
     CDS             <1..2081
                     /locus_tag="PK683_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08398.1"
                     /translation="EGKSSSVRAEEKSERTETAEPKQEKITFPAEPVKVQVKETSAAE
                     KMIPEKISQEMPAEIKPAVNESVSAEENARKTEKTEIDRIIESKKSSIVGLPQKSEEN
                     REEKKKTDTMRVDPAKLDSLMIQASELVVTKNRITNRIREINEVSYYLEELQRFLSES
                     QKLFSALGKGGQNESQKTFILSQLSELNGRQCEKVGSLVSSVNILRKNMGIDISRLGF
                     STMKTERSIYNIRMLPLSTVFSLFHRTVRDMNRETGKNVNLIIEGGETTVDKQILEEL
                     KDPLMHLVRNAIDHGIEKPEDRISAGKPESAELKITGKGFSDTVIIEIKDDGKGIDLE
                     SVKRKAEEKGLFTHDELNAMDRKKLFSIIFMHGFSTRNTVTDLSGRGVGMDVVKSFVE
                     KFKGDIETESEIGKGCRFRIRLPIKFSTTNILITSLGGQKFGFPTDFVVLTRTVQPKN
                     IFQLEGKSAVLIENEPVYLANLSDYLERPEFHSKQIVSSFPCVVLNVDGTKAAVIVDS
                     VIEKNEVILKPFDGILKRVRNVTGAAILDSGEVCIVVNPRDIIDSIFRNSFTVQPTAE
                     KKVPKIKKVLVADDSLTTRVQLKRILENEGIEVSLAVDGLDAWNLISNEDFNCVLSDM
                     EMPNMDGLDLVRKVRDSKYRSLPVIILTSIGSDEKIKETLDAGADAYLVKSKFDRKDL
                     LGVIEKYFNE"
     gene            2074..>2477
                     /locus_tag="PK683_07845"
     CDS             2074..>2477
                     /locus_tag="PK683_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08399.1"
                     /translation="MNDSGKVKIIIIDDSPVVLAILKKIFSSSPDLQIAGTGRNGLEG
                     LELLKMHNPDVICTDLQMPEMDGLEFIRRVMETSPKPMLVISTAVSDAGDKNVFQLLE
                     AGALDVFPKPAGGKEEDYERIGLQLISKVRLL"
BASE COUNT          817 a          461 c          615 g          584 t
ORIGIN      
        1 ctgaggggaa atcatcgtct gtcagggctg aggaaaagtc tgaaaggaca gagacggctg
       61 aaccgaagca ggagaagatt acctttcctg cagaacctgt aaaagtgcaa gtaaaggaaa
      121 cttctgctgc agaaaagatg attcctgaaa aaatttcgca ggaaatgcct gctgaaataa
      181 aacctgcagt gaatgaatct gtttcagctg aagagaatgc aaggaagact gaaaagactg
      241 aaatagacag gatcatagaa agcaaaaaat cttccattgt aggtctgcct cagaagtcag
      301 aagagaacag agaagaaaaa aagaagactg atacgatgag agtggatcct gccaaactgg
      361 attcactgat gattcaggca agtgaacttg tagtgaccaa aaacaggatt acaaaccgaa
      421 tcagagaaat caatgaagtg agttattatc tcgaagagct gcagagattt ctttccgaat
      481 ctcagaaact cttcagtgca ttagggaagg gcggacagaa tgaatctcag aaaacgttca
      541 ttttatccca gctttctgaa ctcaacggaa ggcagtgcga gaaagtcggt tctctggtca
      601 gttctgtcaa tatcctcaga aagaatatgg ggatcgacat cagcagactg ggcttctcca
      661 caatgaaaac ggaaagaagc atctacaata tcagaatgct gcctctgtcc actgtcttca
      721 gtctttttca cagaacagtc agggatatga acagggagac aggcaagaac gtaaacctga
      781 tcattgaagg cggggaaacg acagtggaca agcagattct ggaagaactg aaggatccgc
      841 tgatgcatct cgtgagaaat gcaatagatc acgggataga aaagcctgag gacagaatat
      901 ctgcaggaaa accggaatca gctgaactga agattaccgg aaaaggattc agcgatactg
      961 tcattatcga aatcaaggat gacggaaagg gaattgatct ggagtcagtc aaaagaaagg
     1021 ctgaggagaa gggtcttttt actcatgatg aactgaatgc aatggacagg aaaaaacttt
     1081 tttccattat tttcatgcac ggattttcca ccagaaacac tgtgactgat ctttccggac
     1141 gcggagtcgg aatggatgtg gtgaaaagtt ttgtggaaaa attcaaaggg gacatagaaa
     1201 ctgagtcgga aattggaaaa ggatgcagat tcagaatacg tcttcctata aaattctcca
     1261 ccacaaacat tctgatcact tcactgggag gacagaagtt cggattcccg actgattttg
     1321 tcgtactgac cagaactgtg cagccgaaaa atatttttca gctcgagggc aagtctgcgg
     1381 ttctcataga aaacgaaccg gtgtatctgg caaatctttc cgattatctg gaaaggcctg
     1441 aatttcattc aaagcagatt gtttcttcat ttccgtgcgt tgtactgaat gtggacggga
     1501 ccaaagctgc agtcattgta gattcagtca ttgaaaaaaa tgaagtgata ctgaagccgt
     1561 tcgacggaat actgaagagg gtgaggaatg tgacgggagc tgcaattctg gattcaggtg
     1621 aagtctgcat tgttgtaaat ccgagagaca tcatagattc cattttcaga aacagtttta
     1681 ctgttcagcc gactgcagag aaaaaagttc caaagattaa aaaggtgctt gttgcagacg
     1741 actcactgac aaccagagtt cagctgaaga gaatcctgga aaacgaagga atcgaagtca
     1801 gtcttgctgt tgacgggctc gacgcatgga acctcatcag caatgaggat ttcaactgtg
     1861 tgctttccga tatggaaatg ccgaatatgg acggactcga tcttgtaaga aaggtcaggg
     1921 attcaaaata cagatcgctt ccagtaatca tactgacatc cataggctct gatgaaaaaa
     1981 ttaaggagac tctcgatgcc ggcgcagatg cctatcttgt aaaaagcaag tttgacagaa
     2041 aagatctgct cggcgtaatc gaaaaatatt tcaatgaatg attccggaaa agtaaaaata
     2101 attattatag atgattctcc tgtcgtactg gcaatactga agaagatttt ttcctcaagt
     2161 cccgatctgc agattgcggg caccggaagg aacggtctgg agggtcttga acttttaaaa
     2221 atgcacaatc ctgatgtcat ctgcacagac ctgcaaatgc ctgaaatgga cggtctggag
     2281 tttatcagaa gggtaatgga aacatctccc aagcctatgc ttgtaatcag tacagctgtc
     2341 tctgatgcag gagacaaaaa tgtatttcag cttctggagg caggcgcgct ggatgtgttt
     2401 ccgaaaccgg caggggggaa ggaggaggac tatgaaagaa tagggcttca gctcatcagc
     2461 aaggtcagac ttctgaa
//
LOCUS       DAOQGJ010000428         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27110_length_2476_cov_2.585708, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000428 DAOQGJ010000000
VERSION     DAOQGJ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27110_length_2476_cov_2.585708"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PK683_07850"
     CDS             complement(<1..458)
                     /locus_tag="PK683_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004451934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNH08400.1"
                     /translation="MKPCAHSFVSEDGRRNSPFGWKNPDLPSCEDIVTHIPLLEKFGI
                     PHIFDIHCHFFPEAVMRAVWKWFDKVNWFITYRMSAEKRIDALKRNRVGKFTTLNYAH
                     RPGIAEGLNEWVFQNCSDISEAVLFGTFYPEESAYDYVKKAVEEYGFRGF"
     gene            500..694
                     /locus_tag="PK683_07855"
     CDS             500..694
                     /locus_tag="PK683_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08401.1"
                     /translation="MLILKAVFISFILFFSSSCFIPIVKEIIECERNRDPDYPSAVSN
                     CQQTQKEYDKKHTPEREIKR"
     gene            694..2382
                     /locus_tag="PK683_07860"
     CDS             694..2382
                     /locus_tag="PK683_07860"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNH08402.1"
                     /translation="MQNEIKSLQDRVSALESELSDIRKELNRISGNSSVQEIPKAQQF
                     AEEKEESASDGFFSGLKKKSLENLGSNLFGKAGVLAIILGFVWFLDYAFTNQLLGPSS
                     RIYIGLISSFVLFAGSLHLMKKGSLPLGSAVAGSAFSVLYLSVFSAWFYYDLLSLEET
                     FLSLCVISALPAVLAYISGLQLIYIFSFAASVLSPILLSTGENSYRFFFTYLMLVNLG
                     FLFISRKYIWRFSAFLVYAVNIILYSVWAAKNMEQSSFLVPALFLYSLLCIFLCRESL
                     FQPLLENDSGRKYTVFLLSAFTLSIMYCIISLADHHYPSAKSHFILGAALLYLFSAGI
                     TLKKEISLLQPMILLVLSLLGAFTAVSLYFEGNYRTLSFAGFAGGISASAGILGSSLL
                     LYVSIPFWIFTVLKLMGSDFFIHSSGYFLLNTRFLLFLAGAALAFLTYRKGRNSLGQW
                     ILFFPVTAVFCLVWGSFADVHEFIQDRSVRNLGYSYVLAAYSVPFLAYGFYRNSEVLR
                     KAGLFLLSLLVLKLYMYDIWKMSITVRILAGFSLGGGLLLLSVFYERLKDRFLK"
BASE COUNT          614 a          576 c          497 g          789 t
ORIGIN      
        1 ttgaaccctc tgaaaccgta ttcctcaacc gccttcttca cataatcata tgcagattct
       61 tccggataaa atgtaccgaa cagaactgct tctgaaatgt ctgaacagtt ctgaaacacc
      121 cattcgttca gaccctctgc aattccgggc ctgtgcgcgt agttcagtgt ggtaaatttt
      181 cctaccctgt tccttttcag tgcatcgatt cttttctctg cactcattct gtaagtgatg
      241 aaccagttga ccttatcaaa ccatttccag acagctctca tcactgcctc aggaaagaaa
      301 tgacagtgga tgtcgaatat atgagggatg ccgaatttct caagaagagg aatatgagtg
      361 acaatgtctt cgcaggaagg cagatccgga ttcttccatc cgaaagggga atttcttctt
      421 ccgtcttcgg aaacaaaact gtgagcgcag ggtttcataa aatgattgac aggttctccg
      481 aaaatcagat tatctgacta tgcttatact gaaagccgtt tttatttcct ttatcctgtt
      541 tttcagttct tcctgtttca ttcccatcgt aaaagaaatt attgaatgtg aaagaaacag
      601 ggatccggac tatccttccg cagtctccaa ctgccagcag acacagaagg aatacgataa
      661 aaaacacact cctgaaagag aaatcaaacg ctgatgcaga acgaaatcaa atcacttcag
      721 gacagggtca gtgctcttga gtcagaactt tcggacatca ggaaagaact gaaccggatc
      781 agcggaaact catctgttca ggaaattccg aaagcacagc agtttgcaga agagaaagaa
      841 gaatctgcat cagacggttt tttctcaggt ctgaagaaaa aatcactgga aaatctgggc
      901 tccaatcttt tcgggaaggc aggagtgctc gcaatcattc tcggcttcgt ctggtttttg
      961 gactatgcat ttacaaatca gcttctaggt ccgtcttcta gaatttacat aggcctgatt
     1021 tcctcattcg ttctttttgc aggctccctt catctgatga aaaaaggttc tcttcctctg
     1081 ggatctgcag ttgcgggaag cgcattttcc gttctgtatc tttccgtatt cagtgcctgg
     1141 ttctattacg atctgctttc gctggaggaa acatttcttt ctctctgcgt catctctgca
     1201 cttccggcag ttcttgccta tatatcgggt cttcagctga tctatatatt cagttttgca
     1261 gcctcagttc tttcccctat tcttctttcc acgggagaga acagctacag gttcttcttc
     1321 acttatctga tgcttgtcaa cctgggattc cttttcatca gcagaaaata tatatggaga
     1381 ttttctgctt ttttagtata tgcagtcaat attatactgt actccgtgtg ggccgccaaa
     1441 aatatggaac agagcagctt tttagttcct gctcttttcc tgtactcgct tctctgcatt
     1501 ttcctctgca gagaatccct gttccagcct cttctcgaaa atgattcagg aagaaaatac
     1561 acagtgtttc ttctgtctgc attcactctg agcatcatgt actgcatcat cagtcttgca
     1621 gatcatcatt atccttctgc aaaatcccat ttcatcctgg gagctgctct tctctacctc
     1681 ttcagtgcag gcataacact gaaaaaggaa atcagtctgc ttcagccgat gatccttctt
     1741 gtgctttcac ttctcggagc attcacggca gtctctctgt atttcgaagg aaattacagg
     1801 acactttcct ttgcaggttt cgcaggcggg atttccgcat ctgcaggaat tttaggttcg
     1861 agccttcttc tgtatgtatc cataccgttc tggattttta cagtgctgaa gctcatgggt
     1921 tctgatttct tcattcactc ttccggatat ttccttctga atacaagatt tcttcttttc
     1981 cttgcaggag cagctcttgc atttctgaca tacaggaaag gaagaaattc tctcggacag
     2041 tggatacttt ttttccctgt cactgcagtc ttctgtcttg tctggggaag ttttgcagat
     2101 gtgcatgaat tcattcagga cagatcagtg agaaacctgg ggtattccta tgttcttgcg
     2161 gcctactctg ttcctttcct ggcatacgga ttctacagaa attcggaggt gctgagaaag
     2221 gcaggtcttt tccttctttc tcttcttgtg ctgaaactct acatgtacga catctggaag
     2281 atgagcatca ctgtcaggat tcttgcaggg ttttcactcg gaggaggact ccttcttctg
     2341 agcgtattct atgaaagact gaaagacaga tttttaaaat aactgctgag gactctatga
     2401 aatattttat aatgatatgt gcaattccgg ccgcccttct gtccgccccg ttcaaggaag
     2461 gaaatttcag actgat
//
LOCUS       DAOQGJ010000429         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27390_length_2461_cov_2.701995, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000429 DAOQGJ010000000
VERSION     DAOQGJ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27390_length_2461_cov_2.701995"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1035
                     /locus_tag="PK683_07865"
     CDS             190..1035
                     /locus_tag="PK683_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH08403.1"
                     /translation="MNLSQLELSPETLLKVFSVNIAFLTIRYIIIAGLAYYIFWKLLK
                     DRFQSFRIQEKFPDKEKIITEIKYSLSTFVIFAAVGVGVFTMRKAGYTKIYSNISDYG
                     WAYAVFSFVLLVLVHDTYFYWAHRLMHHKLLFKRVHHVHHYSTNPSPWASFSFHPYEA
                     VLETAFIPAFVIAVPTHPGVMLVFLIAMTVMNVLGHLGYEFWAKGFTRNPWTGWNNTS
                     THHNMHHQKFNCNYSLYFNWWDKICGTNHEEYHNEYDRVTGKRTGTFENQNLAEKPSV
                     KISHA"
     gene            1373..>2461
                     /locus_tag="PK683_07870"
     CDS             1373..>2461
                     /locus_tag="PK683_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08404.1"
                     /translation="MKRLGIILGTVTICFQLSAQTESGRILFKGIQSKDKNSAQMAEK
                     FSISLKGRLEEKSSKNNKTLTSQENTNQEEFPCETDVCLKKALETANTDEMILGFMNR
                     EKDRVRLKMKSVRIQNGILRTLSEFNEMFTDLELLNAHYANEAAEKLLNPSYRIRRIN
                     LAEPSDEKNIKKLEMKSDPSGLKVPNYSSSDDRIARLVSVLEPDLEKADALYNEGKYD
                     AAIDAYTEILLKIAGRLPNDRLPQMRNYAESVSKKLEASYRIILRQHIEYLDIRMNEC
                     RPLKNCDIAELNEWVDSGFKWKAAAPAHMQGRLKMFIPLLNSRRDALQLWGTDLLETE
                     GNELYNKKEYENAILKFEQAERELSSVSD"
BASE COUNT          775 a          501 c          529 g          656 t
ORIGIN      
        1 caacaaggtg ctgcttatgc aagagtattt aaagtagaag ccttaaggcg gggtcttagg
       61 ggttgcaaac ccctaaatct tcccgatagg gaccaacctt tttcttgctt tttttaaaag
      121 caaattattg aattaaatga tgattgtaac tgctgccgct aggctcgcag ttacaaaaag
      181 ggaggaaata tgaatctgtc tcaactcgaa ctcagcccgg aaacactctt aaaggttttt
      241 tccgtaaata tcgcatttct tacaatcaga tatattatta ttgcgggact tgcatattat
      301 atcttctgga agcttctgaa ggataggttt cagagtttcc gcattcagga aaaatttccg
      361 gataaggaaa aaatcattac tgaaatcaag tattctcttt ccacattcgt gatttttgct
      421 gcggtaggag tcggtgtttt tacaatgcgt aaggccgggt atacgaaaat ctattcgaat
      481 atttcagact atggctgggc ttatgcagtc ttcagcttcg tactgctcgt gcttgtacat
      541 gatacatact tttactgggc acacagactg atgcaccata agcttctatt taaaagagtg
      601 catcatgtgc atcattattc cacaaaccct tctccttggg catccttttc attccatccg
      661 tatgaagctg ttctggaaac tgcattcatt cctgcttttg tgattgcagt tcctacacac
      721 cctggagtga tgcttgtctt cctgatcgcg atgacagtca tgaatgtgct cggccatctg
      781 ggatatgaat tctgggccaa aggatttacc agaaaccctt ggaccggctg gaacaatacg
      841 tccactcacc ataatatgca tcatcagaaa ttcaactgca actattctct ctacttcaac
      901 tggtgggata aaatctgcgg aacaaatcac gaagaatacc ataacgaata tgacagagtg
      961 actggaaaaa gaacgggcac attcgagaat cagaaccttg ctgaaaaacc gtctgtaaaa
     1021 atcagtcacg cgtaagaaga gccgcttaag aagagctgct tggcagcaaa cctgaaggcc
     1081 gcctttagct gccttcagga acgtaatctt atttcttccc gtacaagaat tcgtaagtgg
     1141 cagtgattga tgcagtcaaa ctgattagag taatcaaggt ttctgaaatt gctttaaatt
     1201 cagaataggt ggaaataata taattatccc ggaaagccga attccgccca atcatacaag
     1261 caaagactca tctttccagt tgtttattta ccctcatggc tgccatatca gaaaattcaa
     1321 acttgacgca gacttgagtt tttttactgt ctatgagcgg caggagtttt caatgaaaag
     1381 attaggcatt atactaggaa ccgttactat ttgctttcag ctgtctgcgc agacagaatc
     1441 gggaagaatt ctgttcaaag gaattcagtc gaaggacaaa aattccgctc agatggctga
     1501 aaaattttcc atttcgctga aaggcagact tgaagagaag tcttccaaaa ataataagac
     1561 actgacatct caggaaaata caaatcaaga agagtttccc tgcgagacag atgtatgtct
     1621 gaaaaaagct ctggaaacag caaatacgga tgaaatgatt ctcggtttta tgaacaggga
     1681 aaaggacagg gtccgtctga aaatgaaatc tgtccgtatc cagaatggaa tactcagaac
     1741 cctttcggaa ttcaatgaaa tgttcactga ccttgaactt ttgaatgccc attatgcaaa
     1801 tgaggctgct gaaaaactgc tgaacccctc atacagaatc agaagaatca acctggccga
     1861 accttcagat gagaagaata tcaaaaagct ggaaatgaag tcagatcctt caggacttaa
     1921 ggtgccgaat tacagctcca gtgacgacag gatcgcaagg ctggtttcag tgcttgagcc
     1981 ggatctcgaa aaagcggatg cactttacaa tgaagggaaa tatgacgctg caatcgatgc
     2041 ctatactgaa attcttctga aaattgcagg cagacttcca aatgacagac ttcctcagat
     2101 gagaaattat gcggaaagtg tgagcaaaaa actggaagca tcctacagaa ttattctcag
     2161 acagcatatc gaatatctgg acatcaggat gaatgaatgc agacccctga aaaactgcga
     2221 tatcgctgag ctgaatgaat gggtggactc gggctttaaa tggaaggcag cggcaccggc
     2281 tcatatgcag ggccgtctga aaatgttcat tccgctcctg aattcacgta gagacgcact
     2341 gcagctttgg gggaccgatc ttttggaaac agaaggaaat gagctttata ataaaaaaga
     2401 atatgaaaat gccatactga agtttgaaca ggcggagagg gaactttcat ccgtttcaga
     2461 c
//
LOCUS       DAOQGJ010000430         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27501_length_2454_cov_3.914131, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000430 DAOQGJ010000000
VERSION     DAOQGJ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27501_length_2454_cov_3.914131"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(466..1695)
                     /locus_tag="PK683_07875"
     CDS             complement(466..1695)
                     /locus_tag="PK683_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH08405.1"
                     /translation="MNILLLGASGFIGNAIFNSLSSEHKVTAGGRRKLDGMKFWKYAD
                     FSVRTDWDEILQGIDLVINAVGIVEGDFRRIQTETPLELYESCIRKKIRIIHISAIGA
                     EKEKPVSEFLSTKKETDSFLLNYENARIIYPGIVLGKNGKSSQFFAEVASLPAVPVLS
                     DEKMPFIHISQLVILVEEVIKNFIEYPKQIFALSEPEEIQEIYNAMRGRKALFFKIPS
                     FLMNVFFFLFPSARIGIFQKDLFKIMQTVNADDYIKKDNINLAEEDGKIKPSGTVVFP
                     SVSGQLQSSEITASDSLSYAVSLLSVSFIWIWSAVSSLVSWDVSYQLMKETGAGHDLS
                     AASIWAGSIADLVMGYLVFIKKYRKRILLLQILLVLTYTAILTVFASHHWFHPFGTVA
                     KNIPLLALSLHLYLRVR"
     gene            complement(1692..2147)
                     /locus_tag="PK683_07880"
     CDS             complement(1692..2147)
                     /locus_tag="PK683_07880"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HNH08406.1"
                     /translation="MSYLFFKLIHILSATIMIGMGFGSAFYLYFTYRRGNETAVREVL
                     RLVILADTFFTAPSVVIQLVTGILLSEILGLTYSRWFWQVFGISIVIFVLWIRAAYLQ
                     ILMRRILDSGSQMTPEFHRHLQAWFYLGLPSFAGSVILYYMMVYKVSLI"
BASE COUNT          763 a          547 c          456 g          688 t
ORIGIN      
        1 aatactttgg caatccgttg aatttttttg atctccgttc aagatgttcc aggaagtatg
       61 acgggataag gagtgttgaa tttccgcttc ccttcatcgt cagttttgtt ttcagctcct
      121 cactgaacat taacacatta aactcttcag ctgtcatata aagctctcct gaattacact
      181 gtatcgaaaa tgtcgttccg ggtgaaaaaa aaatcagttt tttcactttc aggcagtttt
      241 tcattataag gtgaactgca tttccgaatg agtcttccga gttcctgatg agagtccccg
      301 gaacaggtca gtcagttttt ggagatccta cgaaaaccgg gggctttgga ggagagagac
      361 acgggatagg gatcgaagcg gtgtcaataa gatgcagatg catcttattg acagaagcga
      421 gagcccgggc ttgcgtgata tttcaacacg caagcatccc caaaattacc ttacacgcaa
      481 ataaagatga agactcaaag caagaagagg gatatttttt gcaaccgttc cgaaaggatg
      541 gaaccaatgg tgagaagcaa aaactgtaag tatcgcagtg taggtcagaa caagcaggat
      601 ctgcaggaga aggatccttt tcctgtattt ttttataaat actaaatatc ccatgacaag
      661 atcagcaatg cttcctgccc agatggatgc tgcagacaga tcatggccgg ctcccgtttc
      721 cttcatcagc tgataactta catcccagga aacaagtgaa cttactgctg accaaatcca
      781 tataaaactt acagaaagaa gggaaactgc ataactcagc gaatcagatg ctgttatttc
      841 ggatgactgc agctgtcctg aaacagaagg gaaaacaact gttcctgaag gtttaatctt
      901 cccgtcttcc tctgctaaat taatattatc tttcttaata taatcatcag catttactgt
      961 ctgcataatt ttaaaaagat ctttctggaa tattccaatc cttgcggaag gaaaaagaaa
     1021 gaaaaaaaca ttcataagaa aggaaggaat cttgaaaaac aatgcttttc tccctctcat
     1081 tgcattgtaa atttcctgta tttcttccgg ttcagagagg gcaaaaatct gtttgggata
     1141 ttctataaag ttttttatta cttcttctac aagaatgaca agctgagata tatgaataaa
     1201 gggcattttc tcatcactta gcacaggaac ggcaggcagt gaggcaacct cagcaaagaa
     1261 ctgagaactt ttaccgttct ttccaaggac aatcccaggg taaatgattc ttgcgttttc
     1321 ataattcaga agaaaactgt ccgtttcctt ttttgtagaa agaaactctg atactggttt
     1381 ttctttttca gccccgattg cagaaatatg gatgattcta atcttctttc tgatgcagga
     1441 ctcataaagc tccagcgggg tttctgtctg tattcttcta aaatctcctt caacaatacc
     1501 gacagcattg atgacaagat caatcccctg caggatttca tcccagtcgg tccggacaga
     1561 aaaatcagca tatttccaga atttcatccc gtcaagcttc cgccttcccc ccgctgtcac
     1621 tttatgctcc gaagaaagag aattgaaaat cgcatttccg atgaatcctg atgctcccag
     1681 aagaagtata ttcatatcag tgaaacctta taaaccatca tataataaag aataacagat
     1741 cccgcaaacg atggcagtcc aagataaaac caggcctgca gatgtctgtg aaattcagga
     1801 gtcatctgac tgccgctgtc cagtatcctt ctcatcagta tctgcagata tgctgccctt
     1861 atccagagca cgaatattac gatgctgatt ccaaagacct gccagaacca tcttgaataa
     1921 gtcagcccca gtatttcaga aagaagaata ccggtaacca gctgaatgac aacggacgga
     1981 gcagtgaaaa acgtatcggc caatatgacc agtctcaaaa cttccctgac cgccgtttca
     2041 tttcctcttc tatatgtaaa atacagatag aaggcgcttc caaaccccat tccgatcata
     2101 atcgttgcac tcaatatatg gatcagtttg aaaaacaaat aactcataat atcctcctgt
     2161 cacccaaagc cagtctgtgc ataacacgaa atcactcaat acaagatttc cgaaatttct
     2221 gtttcactgt tcagacctat catacaatat ttcattatat gatcaatatc ctgcagctca
     2281 ttctgctgct caatttctgc tttgtcaagc agcattcatt cagcaggaac ctcacagtct
     2341 gctatgacag ggcagcctaa tcgctgttat cactgaaata atcaacatga aagtccacac
     2401 agcataatct cttcattttg gatagaagcg aaaaatactg ctcggctgcc ttgt
//
LOCUS       DAOQGJ010000431         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27629_length_2447_cov_3.228261, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000431 DAOQGJ010000000
VERSION     DAOQGJ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27629_length_2447_cov_3.228261"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PK683_07885"
     CDS             complement(<1..340)
                     /locus_tag="PK683_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08407.1"
                     /translation="MKTIIFIITAFVFLRCPVHLGIPDRPEYVPVKEETDFLKNKKIG
                     LIGFHTLSIETGKSSLDLSVKTPKYFRLLEYAEVSGISPYGQYIWIEKGSRGYVRKTV
                     AYPDYRRSTAP"
     gene            509..1402
                     /locus_tag="PK683_07890"
     CDS             509..1402
                     /locus_tag="PK683_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08408.1"
                     /translation="MAIALANPIYDTVFKFLLEDLDIARELISEIIHQEIISIQVKPQ
                     ENTVSLEKKDSHKNLRTESADHTSFFTVYRLDFVAEIQTEDGNKKILIELQKAKLATD
                     IMRFRRYLGEQYTKDSLPIITIYFIGYVLDRNLPKVIEVSRLYKDRLTGETAENSRNE
                     FIDSLTHDSFVIQLFGLETKMRNRLEKVLSVFSQAADKLKEHIVYYNYLLDDDLQERI
                     VTRLNYAASDEKLKRQMDLEEEILMELQDLERKIEKQAQTIEEKEKAIEEKEKAIEEK
                     EKTIEEQNRLIQELMKKLDSK"
     gene            1408..2322
                     /locus_tag="PK683_07895"
     CDS             1408..2322
                     /locus_tag="PK683_07895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH08409.1"
                     /translation="MITKDIISKKSFHTSIKKYSCQFFLLSLLLMTVLSAEESPVSGK
                     VFYPPVRDCILHTGEGFADPMYYHSYGSRKYHSGEDYILEWRNEHIISGIQDTESLEK
                     LHEKTFGYLPPADTFSEKDYPEYLKINLYRTGADITDTRIGLYPRSRDDAAYGYGQEV
                     HSVYDGTVEEETSPSEPKGWGKALLIRHDAPPGYAFEIYWEGKMRKLKSFWTQYSHTL
                     KNLKVRGDRVKKGEAVAEIGDGNGIFNTMTGSAFIKEGAHLHFEIRIRKSSLFPDKSL
                     LSNEKKLKETHISPSYFLKNAVLVRDEK"
BASE COUNT          829 a          476 c          494 g          648 t
ORIGIN      
        1 ggggagctgt tgatcttctg tagtcaggat aggcaaccgt cttcctcaca tatcctctgc
       61 tgcctttttc tatccagata tactggccgt aaggacttat tccgctcacc tctgcatact
      121 caagcagtct gaaatattta ggtgtcttta cggaaagatc cagactggac ttgcccgttt
      181 caattgacag tgtatgaaaa ccaatcagtc ctattttctt gttttttaga aaatcagtct
      241 cctcttttac aggcacatac tcaggtctgt ccggtattcc cagatgaaca ggacatctta
      301 aaaatacgaa tgcagtgatg atgaaaatta ttgtcttcat ggaagtctga atattagact
      361 gaatcaccca gagactgaaa attgaactgt ggattatatt gacagaaaaa cagtaaaaaa
      421 ctgctccctt caagtcagat gcaggaatct gaattcttct gccaaatttc ttcttgactg
      481 cttcctgaat ccgatttaat tttcttatat ggccattgct cttgcaaatc caatctatga
      541 cactgtattc aaatttcttc tggaagattt ggacattgcc cgcgaactga tttctgaaat
      601 catacatcag gaaattattt ctattcaggt aaaaccgcag gaaaatacgg tcagtctgga
      661 aaaaaaggat tcccataaaa acttacgaac agaatcagca gaccatactt ctttctttac
      721 agtctaccgt ttggactttg ttgcagaaat tcagactgag gacggaaata aaaaaattct
      781 gatcgaactg cagaaggcaa agctggccac cgatattatg agattccgca gatatcttgg
      841 ggaacagtat actaaagact cactcccaat cattacaatt tattttatcg gatacgtttt
      901 agacagaaac cttcctaaag taattgaggt gtcaagactg tataaagacc gtctgacggg
      961 agaaacagca gaaaatagca ggaatgaatt catcgacagt cttactcacg attctttcgt
     1021 aatccagctc tttggactcg aaacgaaaat gagaaacaga cttgaaaaag ttttgagtgt
     1081 tttcagtcag gcggcggata aattaaaaga acatatagtt tattacaatt accttctgga
     1141 tgatgacttg caggagagaa tcgtaacccg tctgaattat gctgcttctg atgaaaaact
     1201 gaaacgccag atggatctgg aagaggaaat cctgatggaa ctgcaggatc ttgagcgtaa
     1261 aatagaaaaa caggcacaga ccatcgaaga aaaagaaaag gcgatcgaag aaaaagaaaa
     1321 ggcgatcgaa gaaaaagaaa agacgattga agaacagaac agactcattc aggagctgat
     1381 gaaaaaactg gatagcaaat aagggatatg ataaccaaag acataatttc taaaaaatcc
     1441 ttccatactt caataaaaaa atattcctgt cagttttttc tgctttctct ccttttaatg
     1501 accgtcttaa gtgctgaaga atcgcctgtc tccggaaaag tattctatcc cccggtccgg
     1561 gactgcatac tgcataccgg ggaaggcttt gctgacccaa tgtactatca cagctacgga
     1621 tcaagaaaat atcattcagg ggaagattat attctggaat ggagaaatga gcatattatt
     1681 tcaggaattc aggatacaga atcattagaa aaacttcacg aaaagacatt cggctatctt
     1741 ccccctgcag atacattttc ggaaaaggat tatccggaat atctgaaaat caatctgtac
     1801 agaacaggag cagacatcac ggatacaaga atcggactgt atcccagaag cagagatgat
     1861 gctgcatacg ggtacggaca ggaggtgcat tcagtatatg acggaactgt ggaagaggag
     1921 acaagtcctt cagaacccaa aggctgggga aaggctttgc tcatccgtca cgatgctcct
     1981 ccgggatatg catttgaaat ctactgggaa ggaaaaatga gaaaactgaa atcattctgg
     2041 acacagtatt cccataccct gaaaaatctg aaagtaagag gagacagggt aaagaaagga
     2101 gaggcagttg cagagatcgg cgacggaaac ggaatcttca acacaatgac aggatcagca
     2161 ttcatcaaag aaggagcaca tcttcatttt gaaatcagga tcagaaagtc ctcactgttt
     2221 cctgacaaat cccttctttc caatgaaaaa aaactgaagg agactcatat cagtccttct
     2281 tattttctga aaaatgctgt ccttgtcagg gacgaaaaat gaaaatcttc acagtcagaa
     2341 ttatactgat tatttttgtt cttaggtgtt cttcatttca aaaagatgct gacatttccc
     2401 tggaatcagg tgcagaaaaa aagactgatt tttcaatgat tcaaatc
//
LOCUS       DAOQGJ010000432         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27770_length_2440_cov_2.447799, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000432 DAOQGJ010000000
VERSION     DAOQGJ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27770_length_2440_cov_2.447799"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="PK683_07900"
     CDS             complement(<1..1696)
                     /locus_tag="PK683_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08410.1"
                     /translation="MYSFQSVLLTAAALILPLYVFFLASIKENIYSRILFFTLLNLVL
                     CYEGFYCSQEPTVFIYFAAIMVFSFLIFPRKDYSTSILISAVSVLYYALFSFLSSRQS
                     VFQYLLVSDFVIKINLLISFSFLLFSLYWISRNWPDEIQEQFLNSFENYNHSDLALMN
                     SLYEKTLKMNHYLSAFKSAVCSASIVSESDLNGRITAVNDKFCKVTGYSEDELIGQSH
                     NIVNSGFHSKDFFQEIWNTISNGFIWRGEIRNRKKNGEFYWVDSFIIPLLDENEKPIK
                     YISIRNDITDKKRNEEQLIDQKDKAQRLSRQKIEILSMMSHEIRNPLNSILGITELLL
                     DSDLKAEQLKMVSNLKFTADNLIGLVNNILDSHKIESGKLTLEETVFNLELLLKNIID
                     SLYVKARQKNNELVLEVDPQLPRQMMGDPLRLFQIFMNLLSNSIKFTENGYVKTKVYI
                     SSETDDAVTVFAEVSDNGIGIPSDKLDTIFETYSQAEISTSRKFGGTGLGLSIVKSLL
                     ELFHSKIQVESELNKGSRFYFYLTLKKSVQNLDKVEEIKPMQVTELSGIKLLLAEDN"
BASE COUNT          784 a          457 c          451 g          748 t
ORIGIN      
        1 aattatcttc ggcaagaaga agcttaattc cggaaagttc tgttacctgc attggcttga
       61 tttcttcaac cttatccaga ttctgcaccg attttttcaa tgtcagataa aaataaaacc
      121 ttgatccctt gttcagttcg ctctctacct gaattttaga gtgaaacagt tccagaagac
      181 tttttacaat ggaaagtcca agaccagtgc ccccaaattt tctcgatgtg gaaatttctg
      241 cctgagagta cgtttcaaat attgtatcca gcttgtctga aggaatgcct atgccgttat
      301 cggatacctc tgcaaacact gtaactgcat catctgtttc agaggaaata taaacttttg
      361 tcttaacgta accgttctcg gtaaatttga tggaattgct caggagattc ataaaaatct
      421 gaaacagtcg gagcggatcg cccatcatct gtctgggcag ctgaggatca acttcaagaa
      481 caagttcatt gtttttctgc ctggctttaa cgtagagaga atcaataata tttttcagga
      541 ggagttcgag attgaacaca gtctcttcga gagtaagctt tccagattct attttatgag
      601 aatccagaat attgtttacg agcccaatta aattgtctgc agtaaatttc agattactca
      661 ccatcttcag ctgctcagct ttcaaatctg aatccagcag aagctccgta attcctaata
      721 tcgaattcag cggattcctg atctcgtgac tcatcattga aaggatttct attttctgcc
      781 tggaaagcct ctgcgcctta tccttctgat caatcagctg ttcttcattt cttttcttat
      841 cagtaatatc atttcgaatc gagatatatt ttataggttt ttcattctca tcaagaagag
      901 gaataatgaa tgaatctacc cagtaaaact ctccgttttt ctttcggttg cgtatttcac
      961 ctctccagat aaacccattc gatattgtat tccatatctc ctgaaaaaaa tcctttgaat
     1021 gaaaacctga attgactata ttgtgactct gaccgatcag ttcatcttcc gaatatcctg
     1081 tcaccttgca gaacttgtcg ttcactgcag tgattcttcc gttcagatcg gattcagaaa
     1141 caatgcttgc actgcatact gctgatttaa atgcagaaag ataatgattc attttcagtg
     1201 ttttttcata cagactgttc atcagagcca gatcagaatg attataattc tcaaagctgt
     1261 tcaggaactg ttcctgaatt tcgtcaggcc aatttcttga tatccagtaa agactgaaga
     1321 gcagaaagga aaacgagatc aaaagattga tcttaatgac aaaatcagag acaagtaaat
     1381 attggaaaac tgactgtctt gaactgagaa agctgaacag cgcataataa agaactgaaa
     1441 ctgccgagat cagaatactt gtgctgtaat ctttcctggg aaatatcaaa aatgaaaaaa
     1501 ccatgattgc agcaaaatat ataaagactg tcggttcctg actgcagtaa aatccttcat
     1561 aacataaaac cagattgagc agagtgaaaa acagtattct ggaataaata ttttccttga
     1621 tggaagccag aaaaaagaca taaagaggaa gtatcagggc agctgcagtc agaagaacag
     1681 actggaaact gtacacgagg cgaatccgaa aaaattccgg gatataaaag atcattagag
     1741 atatagaaga gaatttaatt aaagaaagag ccttttctgc aaaagaatcc agtgaaaaat
     1801 tcgggcagtt ttctgcggtt aagttcaatg aagagctctc ttttagattt aattacgcca
     1861 attcaataat aaaataaatt ttatgcaaga aaaaattaat tcactgtaaa aatattccgg
     1921 actgtgcttg gttccagctc ttcattacgg cgatcgatga atgaaatatt caatggagac
     1981 ttaatctcat cagtttttac tgtctgtaaa actgcccttt tagaaatttt acaaataatt
     2041 tttcgtctta ataatatttt atatatagta tatttttacg tatgctcagg cggacaaagc
     2101 ttttcggttt tgactgattt gataattcag atatatgttc aatgccgtga cggaatcgga
     2161 ttgcgcttct agcagacttg actgaaggaa gaatgttcag gatagcctct gttcagaaac
     2221 ctgacatcag gcattctaag tttattatgc agatttcgaa ttgcattctg agacggcaat
     2281 gaaggtgagt caaaatactg gacgggtgaa acctttcggt ctttggaaaa cttcaaattc
     2341 tgcagaaaca gctgtaaaat accgtgttca ttcaatataa atgagtggaa accctgtccg
     2401 acgcaagcac tgggcttata gaaaggctga agaattaagc
//
LOCUS       DAOQGJ010000433         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27831_length_2437_cov_3.266583, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000433 DAOQGJ010000000
VERSION     DAOQGJ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27831_length_2437_cov_3.266583"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1409
                     /locus_tag="PK683_07905"
     CDS             345..1409
                     /locus_tag="PK683_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNH08411.1"
                     /translation="MKISFILLALLLQFCNPFEKNKKDDKKPLIFAALAVVTAASSSN
                     TASRSTDTITSKYNSYAPTFTATGNGIYEIVPSVTAAYAAGSLKQGFIDDGVKMANFA
                     RYLAFLPDDLVSDSALNSNGQYGAVLLSASSFSHTPSKPADMDTTFYNEGYSATSSSN
                     IAAGFSTLHSSIQSGYMRDSDTGNMSLVGHRRWILNPQLKKVGFGFAGSSYSLMKVFD
                     KSRTETFDYSYTAWPAEGFFPTEFFSSTDPWSLTLNPKKYKTPTSSSAKVTLTRSSDG
                     KSWTLSSADSTASSTSEYFTVNTGGYGVSNCIIFRPKTTDITSLENDSYTVNVSGIQD
                     ISGNSTTVSYTVKFFKMGIK"
     gene            1494..2132
                     /locus_tag="PK683_07910"
     CDS             1494..2132
                     /locus_tag="PK683_07910"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNH08412.1"
                     /translation="MIFSGMMRMIHSMFIALALVIGLYPAIYFLIDRKFGLLSFKSEV
                     LLNDVFWNFGFYSHIIFGGISLLTGWVQFSQSFREKHLTLHRNIGKIYIISVFISSVS
                     GIYIALNATGGIIASAGFMVLGIIWFTATFSAFTAIRRQDLNMHRSMMIYSYSACFAA
                     VTLRIWLPLLQLIFGNFITAYQAVSWLCWVPNIFIAHFIIKKYPYDETRNNA"
BASE COUNT          720 a          543 c          495 g          679 t
ORIGIN      
        1 gcagaaccga taacccaaaa cactatcaat gcgtaaagag gcaggaaaat atatgcaata
       61 gatgcagttg aagatcggtc ttccgaaatt gatttaacga aggaatacac cgcgtaagct
      121 gttacggcac cggccaaaga aagaataatt acccattcaa gttttgtcat ttttcctctc
      181 tacgaaagtc tgtatgataa aattctgcaa agcttttttt ggcaagcaca aaaaggatcg
      241 gcttgacggt ctaaactaga tttcattttt gtaatttagg gcattttcag gtgtaaagat
      301 gaaaatactg ataatctgta aatatatgac accgaggaga tttcatgaaa atttcattta
      361 tccttctggc tctgctactg cagttctgca acccattcga aaaaaacaaa aaggatgaca
      421 agaaaccgct tatatttgcc gcgcttgctg tagtaactgc tgcctcctcg tctaatacgg
      481 catccagatc gacagataca attacaagta agtacaattc ctatgctccg accttcacag
      541 caacaggaaa cggaatttac gaaatagttc cttcagtcac agcagcatat gcagcgggaa
      601 gtctaaagca ggggttcatt gatgacggtg taaaaatggc aaattttgcg agatatctcg
      661 catttctgcc tgatgatctg gtttccgatt cagccctgaa ttcaaacggt cagtacggag
      721 cggttctgct ttcagctagc agtttctctc atactccttc aaagccggca gatatggata
      781 caacatttta taatgaaggg tactctgcaa cttcttcgag caatattgct gcaggatttt
      841 ccaccctcca ttcctccata cagtcaggtt atatgagaga ctctgacacc ggaaatatgt
      901 cgctggtcgg gcacagaaga tggatactca atccgcagct gaaaaaggtc ggattcggat
      961 ttgcaggaag cagctattct ctgatgaaag tatttgacaa gagcagaaca gaaacattcg
     1021 actacagtta cactgcctgg cctgcagaag gattttttcc cactgagttc ttttcgagca
     1081 cagatccctg gtcactgact ctcaatccta aaaaatacaa aacgccgacc agcagttccg
     1141 caaaagtcac tctcaccaga agttccgacg gcaaatcctg gacattgagc agtgcagaca
     1201 gcaccgcaag ctccacaagt gaatacttca cagtcaatac gggaggatac ggagtcagca
     1261 actgcatcat cttccgtccg aaaacaacgg acatcacttc actggaaaac gattcctata
     1321 cagtcaatgt cagcggaata caggacatca gcggaaacag cactactgtc agttatactg
     1381 tcaagttctt caagatggga attaaatagt tcgtcggatg caggcagcag ttcagtccgc
     1441 ttgaggaaac agcagaataa cgttatgaaa aattctttgt tcagggaaga cagatgattt
     1501 tcagcgggat gatgagaatg atacattcaa tgttcattgc actcgctttg gtcattggac
     1561 tttatcccgc aatctatttt ctgatagacc gaaagttcgg tcttttaagc ttcaaatcag
     1621 aggtcctcct gaatgatgtt ttctggaatt tcggtttcta ttcacatata attttcggag
     1681 gaatttccct actgacaggc tgggtgcagt tcagtcaaag cttcagagaa aaacacctga
     1741 ctctgcacag aaacattggg aagatctata ttatctctgt ctttatcagt tctgtttcag
     1801 gtatatatat tgcactgaat gcaaccggag ggattatcgc atctgcagga ttcatggtcc
     1861 tgggaatcat ctggtttacc gcaacttttt cagcattcac tgcaataagg aggcaggatc
     1921 tgaatatgca caggagcatg atgatataca gttattctgc ctgttttgcc gcagtcactt
     1981 taagaatctg gcttcccctc ctgcagctga tattcggaaa tttcatcact gcctatcagg
     2041 cagtttcctg gctctgctgg gttcccaata tttttattgc acatttcatt ataaaaaaat
     2101 atccctatga cgaaactcgg aataatgcct gattcttcag gcacactgtc atctttttgc
     2161 agaaatccac tcagcccctc agttcacaga gccttattcc tttccttatt ctttttttta
     2221 tactgctttc acccggcaaa tgaaagcttt cctggaggag agggcaccag tttcgatttt
     2281 tccctgaatg cattcaacag acatatcacc aacctgaaag acaatgataa aataatgcag
     2341 ttcaacagag gaaatcagat attcaaggcc gtctggtcga atgacggagg gcctgtattt
     2401 caaggtctgg gacctttatt caattccaga tcctgca
//
LOCUS       DAOQGJ010000434         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27832_length_2437_cov_3.224601, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000434 DAOQGJ010000000
VERSION     DAOQGJ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27832_length_2437_cov_3.224601"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK683_07915"
     CDS             <1..333
                     /locus_tag="PK683_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH08413.1"
                     /translation="LIERKYSYGDMQNFDIIYCGINDETERKKIRTEAEEKKKLINFV
                     DRPLDSDFISASSLIYDNFTIFISTYGRSPGGAKKIRETLEAKMSLETLNDEIGEMIR
                     KRNAVQSY"
     gene            314..1039
                     /locus_tag="PK683_07920"
     CDS             314..1039
                     /locus_tag="PK683_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNH08414.1"
                     /translation="MQFSHIKRFQENTVYLIGGGPGDIGHLTMDAVSILSQSEVILHD
                     MFLDSLHPNFSQAEWIHVGKQKGHHIKKQSEINEMLFHYVSSGKKTVRLKAGDASFFA
                     RSSEEIEYLSERNVRTSIIPGISSPQLLCEELNDSLTHRTLTRNLSFWSGYWDKEISE
                     NETPLTDGHIIFMGSGQLQKIAEKLHSKGIPLNENIIIASNLGRNEKKISHITIQDAI
                     SGNIPEMQSPSLIAVGFRHIVKP"
     gene            1036..1782
                     /locus_tag="PK683_07925"
     CDS             1036..1782
                     /locus_tag="PK683_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002746374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH08415.1"
                     /translation="MRIGLVKHSNARPLTFGIEKSGEHSLVYENPSVLKDLLLEGKLD
                     TALISSVECIRNRGLSYTTCVGVAAAEKVRSILFFRNRKMKNTEKIYTDHGSRSSVAL
                     LRILIAETEGRLPETEPTLPEKIQEMIHQGDGHHLLFGDNALRADWDPEKYEAHDLAS
                     FWHQFTGKSFCFAFWAYPKENPVNDSIFLNSLEYGLSHIDDIVNEEKNLDRTMLNHYL
                     RYELHYKINNLDLEGFGLFKELLQKYGLDK"
     gene            complement(1807..>2437)
                     /locus_tag="PK683_07930"
     CDS             complement(1807..>2437)
                     /locus_tag="PK683_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNH08416.1"
                     /translation="VAGILKKMKSRTRLPVTVKHRIGVNGKETYEDMKEFVSIVSASG
                     CDRFIIHARIAVLGGLSTKENRTIPPLRYQDVYRLKEEFPHLKIEINGGIKDWANAAE
                     HLKKTDGVMIGRSAYEDTYLFASADQKIFNSRENILSRKQLLYSIVPYMENMMRNGIP
                     ARKILTHTLNLFSGLPGARKYRQYLSENMPRIADGIVLITEALKLVEEG"
BASE COUNT          755 a          515 c          490 g          677 t
ORIGIN      
        1 ctgatcgaga gaaaatattc gtatggagat atgcagaatt ttgacatcat ctactgcgga
       61 atcaatgatg agacggagag aaaaaaaatc agaaccgaag cggaagaaaa aaagaaactg
      121 atcaattttg tagacagacc tctggattcc gatttcatat cggcatcctc tctgatctat
      181 gacaacttta ctattttcat atccacatac ggcagatcac cgggcggtgc aaaaaaaatc
      241 agagaaactc ttgaagcaaa aatgagtctg gaaaccttga atgatgaaat cggtgaaatg
      301 atccggaaaa gaaatgcagt tcagtcatat taaacggttt caggaaaaca cggtatatct
      361 tatcggagga ggaccggggg atatcggtca tctgacaatg gacgcagtgt ctattctttc
      421 tcagtcagaa gtcattctgc acgatatgtt tctggattct cttcatccga atttttctca
      481 ggcagaatgg attcatgtag ggaaacagaa aggccatcat ataaaaaaac agtctgagat
      541 caatgaaatg ctttttcatt atgtttcttc aggaaaaaag acagtcagac tgaaggcggg
      601 agatgcttcc ttttttgcaa gaagctctga agaaattgaa tatctttctg aaaggaatgt
      661 ccgcacttcc attatcccgg gaatttcgag tcctcagctt ctctgtgaag aactgaacga
      721 ctctctgact cacagaactc tcactaggaa cctttctttc tggagcggat actgggacaa
      781 agaaatcagc gagaatgaga ctcctctgac agacggtcat atcatattca tgggatcggg
      841 acagctgcag aaaatcgcag aaaaacttca ttcaaaagga attcctctga atgaaaatat
      901 cattatagct tcaaatctcg gcagaaatga aaaaaaaata tctcatatca caatccagga
      961 tgcgatctca gggaatattc ctgaaatgca gagtccctcg ctgattgccg taggcttccg
     1021 ccacattgta aagccatgag aataggactg gtcaaacaca gcaatgcaag acctctgact
     1081 ttcggaatag aaaagtcggg agagcacagt cttgtctatg aaaatccatc agttctgaaa
     1141 gatctccttc tggaagggaa actcgacacc gcactgattt cttctgtgga atgcatacga
     1201 aacagaggtc tttcctacac aacctgcgtg ggagtggctg cagcagaaaa agtcagatct
     1261 attctgtttt tcagaaaccg caaaatgaaa aatactgaaa aaatatatac agatcacggt
     1321 tccaggagca gtgtcgctct tttaagaata ctcattgcag aaaccgaagg cagacttcct
     1381 gaaacagaac ccacactccc tgaaaaaata caggaaatga tccatcaggg agatggacat
     1441 catcttcttt tcggagataa tgcacttcgt gcagactggg atccggaaaa gtatgaggcg
     1501 catgatctgg cctctttctg gcatcagttc acgggaaaat cattctgctt tgctttctgg
     1561 gcatatccga aggaaaatcc ggtaaacgat tccatttttc tcaattcgct tgaatatgga
     1621 ctttcgcata tagacgatat cgtaaacgag gaaaaaaatc tcgacagaac gatgctgaat
     1681 cattatttac ggtatgaact tcattataaa ataaacaatc ttgatctgga aggattcggt
     1741 cttttcaaag aacttctgca aaaatatgga ctggataaat aaaccggctt ccctgctgaa
     1801 atctccttat ccttcctcca ccaattttaa tgcttctgta attaaaacta tcccatctgc
     1861 aatcctcggc atattctcgg aaagatactg cctgtatttt cttgcaccag gcagaccgga
     1921 aaaaagattc agggtatgtg taagaatttt tcgtgcagga atgccgttcc tcatcatatt
     1981 ttccatgtac gggacaatgc tgtaaagcag ctgctttctg ctgagaatat tttccctgga
     2041 attaaagatt ttctgatctg cactggcaaa aagataagtg tcttcatatg ctgaacggcc
     2101 aatcattaca ccgtctgttt ttttcagatg ttctgcagca ttcgcccagt ccttgatacc
     2161 tccattaatc tcaattttca gatgaggaaa ttcttccttg agtctgtata catcctgata
     2221 ccggagcgga ggaattgtcc tgttttcctt tgtgctgagt ccgccaagaa ctgcaattct
     2281 cgcatgtatg ataaatctgt cgcatccgga tgcagaaacg atgcttacaa attctttcat
     2341 atcttcatac gtctcctttc cattgacacc tatacggtgt ttcactgtca caggtagtct
     2401 tgttctgctt ttcatctttt tcagaattcc agcaacc
//
LOCUS       DAOQGJ010000435         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27973_length_2430_cov_3.162526, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000435 DAOQGJ010000000
VERSION     DAOQGJ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27973_length_2430_cov_3.162526"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..830)
                     /gene="fabG"
                     /locus_tag="PK683_07935"
     CDS             complement(72..830)
                     /gene="fabG"
                     /locus_tag="PK683_07935"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNH08417.1"
                     /translation="MIDFKNKSVIVTGSARGIGKAIALKFAELGANLVITDINEEATK
                     ATAEEFKSKGYKAIAVVCNVAKEEDTIALAEACIKEYGTLDVLINNAGITKDTLLMRM
                     KKEQWDAVIAVNLTGVYNCTQAVTKQMMKQRSGAIVNMTSVVGIGGNAGQANYAASKA
                     GVIGFTYSCAKELASRNIRVNAIAPGFIETDMTAAIPENIKEGMTKAIYLGRTGKPDD
                     IANAAVFLSSDAASYISGIVLPVHGGGFREGGGH"
     gene            complement(840..1844)
                     /gene="plsX"
                     /locus_tag="PK683_07940"
     CDS             complement(840..1844)
                     /gene="plsX"
                     /locus_tag="PK683_07940"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNH08418.1"
                     /translation="MWVAVDTMSGDLGPIPLVEGSIDAINEFGYSVVLVGQEEILGEL
                     ILRYEFDSSKLKIVHASEVIDMNDSPSIAVKAKPDSSIVQSMQLVASRECVGVFSPGN
                     TGASMAAALMHLGRIPGVLRPPIAVPLPQQDGDTMLLLDAGANVDCKPEYLVQFALMG
                     EIYAREILGIPKPKIGILSNGEEDKKGNSQSLKVFDIMKFLPINFVGNVEGRDLYGSG
                     KDVDVVVCDGFVGNIVLKATEGLAKSIFNVLKKNIEDSSIAKTGALLLKPTFKAVKKR
                     LDFEEHGGAVLLGVNGTCIIGHGTSTAIATKNAIRVVVECARNDVNVRIAENLIKFKT
                     "
     gene            complement(1850..2062)
                     /gene="rpmF"
                     /locus_tag="PK683_07945"
     CDS             complement(1850..2062)
                     /gene="rpmF"
                     /locus_tag="PK683_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNH08419.1"
                     /translation="MAAPKRRKSKSKTRMHRAHHAIGIPNLVPCSNCGAFILPHRVCP
                     ACGFYNGKLAVPPKVKKSKDNSTSEE"
BASE COUNT          612 a          591 c          475 g          752 t
ORIGIN      
        1 aaaaaagccc gcgacaagtg ccgcaggctt ttaatgtttt ttaattacga aaaattattt
       61 tgtatgtacg atcagtgacc gccgccttct ctgaaaccgc cgccgtgaac aggaagaacg
      121 attcctgaaa tgtaggaagc cgcatcgctg gaaaggaata ccgctgcatt tgcgatatca
      181 tccggttttc ctgttctgcc gagataaatt gccttagtca ttccttcttt gatgttttct
      241 ggaattgcag cagtcatatc tgtttcgatg aatccgggtg ctatcgcgtt tacacggatg
      301 tttctggatg caagttcttt tgcacaggaa taagtgaatc cgatcacgcc tgctttggat
      361 gcagcatagt ttgcctgacc tgcgtttccg ccgataccga ctacagaagt catattgaca
      421 attgcaccgc ttctctgctt catcatctgc ttagttacag cctgagtaca gttgtaaacg
      481 cctgtcagat tgactgcaat aacagcatcc cactgctctt ttttcattct catcagaaga
      541 gtgtcttttg taatacctgc attgttgatc agaacatcca gtgttccgta ttcttttatg
      601 caagcctcag caagagcaat tgtatcttct tccttagcca cattgcatac tactgcaatt
      661 gctttgtaac ctttggactt gaactcttca gctgttgcct ttgttgcttc ttcgttgata
      721 tctgtaatta caagattcgc tcctagttca gcaaacttca gagcaatcgc cttcccgatc
      781 cctctggccg aaccagtcac tattacagat ttatttttga aatcaatcat ctccatctcc
      841 taggttttga attttatcag attttcagca atgcgcacgt tcacatcgtt ccgggcacat
      901 tccactacta cacgtattgc attttttgtg gcgattgcag tcgaggtccc gtgtccgata
      961 atgcaggttc cgttcacgcc aagtaatact gctccgccat gttcttcaaa gtccagtctt
     1021 tttttcactg ccttgaatgt aggcttgaga agcagtgctc cagtcttcgc aatgctggaa
     1081 tcttctatat tttttttcag tacgttgaaa atggattttg caagtccctc tgtagccttc
     1141 agcacaatat tccccacaaa tccgtcgcag acaactacat ccacatcctt gccgctcccg
     1201 taaagatctc tcccctccac gttgcctaca aaattgatcg gtagaaattt cataatatca
     1261 aaaactttca gtgactgtga attccctttt ttatcctcct ctccgttgga gaggattcct
     1321 atcttcggtt taggaattcc taaaatttct ctggcataga tctcgcccat cagtgcaaac
     1381 tgcaccagat actccggctt gcagtccaca tttgcccctg catccagaag aagcatcgta
     1441 tctccgtcct gctgcggaag cggaaccgca atcggcggcc tcaatacacc gggaatcctg
     1501 cccagatgca tcaacgccgc tgccatagaa gcacctgtgt ttccaggaga aaatactccg
     1561 acacattcgc gggaagcgac cagctgcata gactgaacaa tggaagaatc aggtttggct
     1621 tttactgcaa tggaaggaga gtcgttcatg tctatgactt cagaggcatg aactatcttg
     1681 agttttgaac tgtcgaattc gtatctaagg atcagttctc cgagtatttc ctcctgccct
     1741 accaggacaa cggaataccc gaattcattg attgcgtcaa tggagccttc cactaaggga
     1801 attggcccca aatcgccgct cattgtatcc acggcgaccc acatgactct tattcttcag
     1861 atgtgctgtt atcttttgat ttttttactt ttggaggaac tgccagtttg ccgttgtaaa
     1921 aaccgcaggc cggacagacc ctgtgtggaa gaatgaaagc accgcagttg ctgcaaggca
     1981 ccagatttgg tattcctatg gcatggtgtg ccctatgcat tcttgtcttg gatttcgatt
     2041 tgcgtctctt tggagctgcc attttttcct ctgaacccta cttaatatat cagaattcca
     2101 agatttttta caccttgaat ttcgcaagta aaataatcgt gtactctttt ttgagagggt
     2161 tgggggaggg tcttatggac agaccgacat tttccctgca ggaaaataaa agaactgaat
     2221 tcggagagcc gaattccaga aattactcct tctaaaaaca ctgtattctg tttctttatg
     2281 caaatctaag attaaagata cttttacgga gccgcaaatt ctaattgtac tgcaagaata
     2341 attactgatg acgtttgtta tccggaatca gcaaattgaa cctaccccat tttttagtaa
     2401 cgcatttttt tcttttcaaa agaaaaaaca
//
LOCUS       DAOQGJ010000436         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_27975_length_2430_cov_2.982316, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000436 DAOQGJ010000000
VERSION     DAOQGJ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27975_length_2430_cov_2.982316"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK683_07950"
     CDS             <1..452
                     /locus_tag="PK683_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08420.1"
                     /translation="NIYELQLETEPGKIQIISKNLVSRVSYEEYVARKTEPPPAKKEE
                     PPKTDPEPMVYQVKKDNKTYLIDVDMGHLPLIFVATLVRGELIIPQEVRNATDKYFLL
                     VVNPDLLLTGDYDLVIRSIDGKEISRKTNFVSVVLPEDPKVVEQKQP"
     gene            433..1038
                     /locus_tag="PK683_07955"
     CDS             433..1038
                     /locus_tag="PK683_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH08421.1"
                     /translation="MNRSSPKDLLLIPNILTLLRILLIFPGIFLIHSGNRAGAVITVT
                     VLFLTDFFDGYLARKLNQTSYIGSILDPVADKFVVLSFFTFFYFSGTVPLFYFLLILV
                     RDIAQLLSVPVLLLWKKISFKVAPKLTPKIGTALNFVILGTAGFVFLFPDIQFSTSIQ
                     SPEMIFMILVYAVSSAIELYILFTYIPRFVQIYRGTHDTFE"
     gene            1054..1248
                     /gene="thiS"
                     /locus_tag="PK683_07960"
     CDS             1054..1248
                     /gene="thiS"
                     /locus_tag="PK683_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNH08422.1"
                     /translation="MNGKEIALSELKNGSLIDLFEKFSLNPKASAVEINGQIIDRENF
                     ADRRLSENDTVEIIRFVGGG"
     gene            1250..1915
                     /locus_tag="PK683_07965"
     CDS             1250..1915
                     /locus_tag="PK683_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNH08423.1"
                     /translation="MKKKWNSSGLYPILDYDYCLKKEIDIFQILRIWLSCPDYVECIQ
                     IRAKSLTEKKYTHLFNEIQTSFPDALFIINDHWKLYRKFPAYGIHLGKEDYQSLTESE
                     KEEFADIKMIKGTSSHSVEDLKNLDGNLWTYTGFGPMFATETKKSDYETLGLRALLTA
                     SEFSPVPLVPIGGITLSSFPEIYRAVKCRPASVSLYSHIPSFRKIISFLKGQNTAIKS
                     EIL"
     gene            2003..>2430
                     /locus_tag="PK683_07970"
     CDS             2003..>2430
                     /locus_tag="PK683_07970"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNH08424.1"
                     /translation="MKQPFEVVHNSAKEEKDYVLEYQKIDVTERPKLTLDGVEFDNIT
                     RDHAVAVILDYIKKKERFHHILFLDPVKFMNVRETEPLHRITKKTSMILAESGGLSWA
                     ASKTGQSLVEHISIISLMMDLIRYSEKQGLTIFFLGSKEKT"
BASE COUNT          723 a          514 c          488 g          705 t
ORIGIN      
        1 agaatatcta tgagcttcaa ctggaaaccg aaccaggaaa gattcagatc atctccaaga
       61 atctggtgag tagagtcagt tatgaagagt atgttgccag aaagacagaa cctcctccgg
      121 caaaaaagga agaacctccg aaaactgatc cggaaccgat ggtttaccag gtgaagaaag
      181 acaacaagac ttatctgatt gatgtggata tgggtcatct cccccttatt ttcgttgcca
      241 ctctagtcag aggagaactg atcattcctc aggaagtcag aaatgcaacg gataagtatt
      301 ttcttctggt agtaaatccc gaccttcttc tcacgggaga ctatgatctt gtcatccgtt
      361 ccattgacgg aaaagaaatt tccaggaaga caaattttgt atccgttgtg cttccggaag
      421 atccaaaagt agttgaacag aagcagccct aaagaccttt tactgattcc gaatattctg
      481 acccttctca gaatattatt gatttttccc ggaatctttc tgattcattc agggaacagg
      541 gcaggtgcgg ttattactgt gactgtcctt tttctgacag atttttttga cggatatctg
      601 gcaagaaaat tgaatcagac atcatacatc ggttccatcc ttgacccagt ggccgacaag
      661 ttcgtcgttc tttcattttt cactttcttc tatttttcag gaacagttcc tctgttctat
      721 tttcttctga ttctcgtcag ggatattgct cagctgcttt ccgtaccagt gcttcttctc
      781 tggaaaaaaa tcagcttcaa agtcgccccc aagctgactc ccaagatagg gacagctctg
      841 aactttgtca tcctgggaac agcaggcttc gttttccttt tccctgacat tcagttcagc
      901 acatccattc agagtccgga aatgattttt atgattctgg tctacgcagt ttcttctgcg
      961 atagagctct atattctctt tacttatatt ccccgatttg tacagatata cagaggaacc
     1021 catgatacat ttgaatagga ttcaaaaatg acagtgaacg gaaaagaaat tgcactcagt
     1081 gaactgaaga acggaagtct cattgacctc tttgaaaagt tttcactcaa tccgaaagct
     1141 tctgcagtgg aaatcaacgg tcagattatt gatagagaga attttgctga cagacgtctt
     1201 tctgaaaatg acactgtaga aatcattcgt tttgtaggag gaggctgaaa tgaaaaaaaa
     1261 atggaattca tccggtttat atcccatttt agattatgac tactgcctga aaaaggaaat
     1321 cgacatattt cagattctcc gaatctggct ctcctgtcct gattatgtgg aatgcattca
     1381 gatcagagcg aaatcactca ctgaaaaaaa atacactcat cttttcaatg aaatacagac
     1441 cagttttccc gatgcacttt ttatcatcaa tgaccactgg aaactgtaca ggaagtttcc
     1501 tgcttacgga attcatctgg gaaaggaaga ctatcagagt ctgactgaat cggaaaagga
     1561 agaatttgcc gatatcaaaa tgatcaaagg aacttcctct cacagcgtgg aagacttgaa
     1621 aaatctggac gggaatctct ggacctatac cgggttcggg cctatgtttg ctacagagac
     1681 taaaaaatca gactacgaaa ctcttggact gagagctctt ctgactgcct ccgagttttc
     1741 cccggttcct cttgttccga tcgggggaat cactctttcc tcttttcccg aaatctatag
     1801 agcggtaaaa tgccgcccgg catcggtcag tctgtactct catatccctt ccttcagaaa
     1861 gattatcagc tttttaaagg gacagaatac tgcgataaag tcagaaattc tttagcataa
     1921 aattctgtat tatttggaaa aatggacatt tgtttttttt aattttcctt gtaaattatc
     1981 tctcagaaat gaggctttgc atatgaagca gcctttcgaa gtagttcaca attccgcaaa
     2041 ggaagaaaag gactatgttc tcgaatacca gaagatagat gtgaccgaga gacccaaact
     2101 cactctggac ggagtcgagt ttgacaatat cacaagggat catgcagtcg ctgtcattct
     2161 ggactacatc aagaaaaaag aaagattcca ccacattctt tttctggacc ctgtgaaatt
     2221 tatgaatgtt cgggaaactg aaccgcttca caggatcaca aagaaaactt ccatgattct
     2281 tgcagaatca ggcggacttt cttgggcagc atccaagaca ggacagtctt tagtggagca
     2341 tatttccatc atcagcctga tgatggatct gatacgttat tcagaaaaac agggactcac
     2401 tatatttttt ctgggaagca aggagaagac
//
LOCUS       DAOQGJ010000437         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28009_length_2429_cov_2.259899, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000437 DAOQGJ010000000
VERSION     DAOQGJ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28009_length_2429_cov_2.259899"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK683_07975"
     CDS             complement(<1..586)
                     /locus_tag="PK683_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase YidC"
                     /protein_id="HNH08425.1"
                     /translation="MEDRQSKLLLAIILGFGIWMTINYVFFPQPKETPKKKDPVAKEV
                     SNPAEVKPDNKEVPSKQTKETASKDEKKPVKEKLEAPAKKIEVKDYRIITSSFLAELS
                     SEGGRIKKFYVKNYKDMDGKDVVIAKGENDTVQFDGQTFKAIEVSREKGFDFNIIESQ
                     EEIPISPYNHTVYESEFDQQKLTAVFRTVSTDRKY"
     gene            complement(634..849)
                     /gene="yidD"
                     /locus_tag="PK683_07980"
     CDS             complement(634..849)
                     /gene="yidD"
                     /locus_tag="PK683_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNH08426.1"
                     /translation="MKYIAILFIKFYKKLISPFLPPVCRFVPSCSEYAMLSYERHGFL
                     LGSFYSAKRICRCHPGNPGGLDPVPEK"
     gene            complement(1173..1334)
                     /gene="rpmH"
                     /locus_tag="PK683_07985"
     CDS             complement(1173..1334)
                     /gene="rpmH"
                     /locus_tag="PK683_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNH08427.1"
                     /translation="MKRTYQPSKIKRVRTHGFRSRMATPGGRNVLSRRRLKGRHKLTV
                     SDEKIGKKF"
     gene            complement(1542..2156)
                     /locus_tag="PK683_07990"
     CDS             complement(1542..2156)
                     /locus_tag="PK683_07990"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNH08428.1"
                     /translation="MFTGLVETLGKICEITKEKGGAFFSIEADFENNDLKNGDSVSVN
                     GACQTVISLSDDRRIFRIYSSFKTLELTNFSSLKMGEKVNLERAMLPTSRMGGHIVQG
                     HVDGIGRILSRKSAEDGLSEIFIVEIPKPLSLYAVSRGSITVDGISLTVVEAEENRVH
                     LVLIPETVRKTNASEWKAGKEVNVETDILARYAEKFILKDRNTL"
     gene            complement(2159..>2429)
                     /locus_tag="PK683_07995"
     CDS             complement(2159..>2429)
                     /locus_tag="PK683_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08429.1"
                     /translation="AAVLFTGFTTFGIVSFLSQDKLLLDERTFRDIGDFFFGKTAMAG
                     NSFRIFLEYFDPSFHPSQKDIDSLAQEVFKELNEKYPKPLSLVVN"
BASE COUNT          651 a          534 c          441 g          803 t
ORIGIN      
        1 tgtattttct gtctgtggaa actgtcctga atactgcagt cagcttctgc tgatcaaatt
       61 cagattcgta aacagtatga ttataaggac ttattggaat ttcttcctga gactctatga
      121 tgttgaaatc aaatcccttt tctctcgaaa cctcaatcgc cttgaaagtc tgtccgtcaa
      181 attggacagt atcattttct cctttcgcta tgacgacatc ctttccgtcc atatccttgt
      241 aattcttcac gtagaatttc tttattcttc cgccctcgga actcagttca gccagaaagg
      301 aagacgtaat gattctgtag tcttttacct ctattttttt tgcaggggct tccagctttt
      361 cctttacagg ctttttctca tcctttgaag cagtctcttt tgtctgcttg gaaggaactt
      421 ccttgttgtc aggttttact tcagcagggt ttgagacttc ttttgcgact ggatcttttt
      481 tctttggagt ttctttgggc tggggaaaga atacatagtt gattgtcatc caaatcccaa
      541 atccgagtat tatagctaga agcagcttgg attgtctatc ttccatttaa gtttttatcc
      601 tttgtacagt aatagtattg atctgtcttc catttatttt tcaggcactg gatcgagacc
      661 tccgggattt cccggatgac atctgcagat tctttttgcg gagtaaaatg acccgagcaa
      721 aaagccgtgt ctttcatagg agagcattgc atattcagag caggatggaa caaatctgca
      781 tacaggcggc agaaaaggag agatcagctt tttataaaat ttaatgaata atattgcaat
      841 atatttcatt gaatctttga tagaagtgat tccagtgctt ttactctgaa ttcatatttc
      901 atttttgtaa attccagatt tgctgtcaaa gccaagtcaa aatgatcagg aacaggtaaa
      961 tattcagtat tcagtattgc ccggagtact cgcttgatcc tgtttctatc cacagccgac
     1021 ttagtctttc gttcagaaca gtaaagtact ctgactgagc tgaccgtatt tctccggtat
     1081 ctgacagaca ggccctcagt cttaacgagt cttcctgtcc tgaacagatt cttgatatcc
     1141 ctgctcgtcc gaagaacagc aaatgacaat tcttagaatt tttttccgat tttttcgtct
     1201 gaaactgtta atttatgtct tcctttcagt cttctgcggg aaagcacatt tctgccgccc
     1261 ggagtcgcca ttctggaacg aaatccatgt gttcttactc ttttgatttt acttggttgg
     1321 tatgttcttt tcatacagtt ccctacttgc gaaccataag cctaacggct taaacggttc
     1381 gaaatgattg cagtctgaga tttcattgaa atcttcaatt tccgttcaaa gaccattttt
     1441 tatgatcccg aaaaggtata atatttacca attccctgtc ggacaggtta ctgtcaattt
     1501 ctaaaccggt aaaaaatcca ttttttcaaa ggactgacag ctcaaagagt atttctgtcc
     1561 tttaaaatga acttttcagc atatcttgcc agaatatctg tttcaacgtt tacttccttc
     1621 cctgccttcc attcggatgc attcgttttc ctgactgtct cgggaatcag taccagatgc
     1681 actctgtttt cctctgcttc cacaacagta agagaaattc cgtctacagt gatgctccct
     1741 cgagaaactg catacaatga aagaggctta ggaatttcga cgataaatat ctcggacaga
     1801 ccgtcttcag cactttttct cgaaagaatc cttccgatcc cgtccacatg accctgtaca
     1861 atatgtcccc ccattctgga tgtaggaagc atagctcttt ccagattcac cttctctccc
     1921 attttcaatg aggaaaaatt ggtcagttcg agtgtcttga aactggagta gattctgaat
     1981 attcttctgt cgtctgaaag ggaaataaca gtctgacagg caccgttcac tgaaacagaa
     2041 tcaccgtttt tcagatcatt attttcaaaa tcagcctcaa tggaaaaaaa agctccccct
     2101 ttttcctttg tgatttcgca gatttttccg agtgtttcca caagtccagt aaacataatc
     2161 aattcaccac cagagaaagc ggcttcggat atttttcatt gagctcttta aatacttcct
     2221 gtgcaaggct gtctatatcc ttctgcgaag ggtgaaagga aggatcaaag tactccagaa
     2281 agatccggaa tgaatttccg gccattgccg tttttccaaa gaaaaaatca ccaatatccc
     2341 gaaaggttct ctcgtccaaa agaagtttgt cctgagaaag aaaacttacg attccaaaag
     2401 tcgtaaaacc ggtaaaaaga acagctgca
//
LOCUS       DAOQGJ010000438         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28126_length_2422_cov_2.324039, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000438 DAOQGJ010000000
VERSION     DAOQGJ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28126_length_2422_cov_2.324039"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK683_08000"
     CDS             complement(<1..214)
                     /locus_tag="PK683_08000"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNH08430.1"
                     /translation="MKKSLFYHVFILLFFFASCLKAQESQGFQEVKAWMGVSIEDKPE
                     GVFVNRAIPGTPAAKAGLKPGDIIRKL"
     gene            complement(355..717)
                     /locus_tag="PK683_08005"
     CDS             complement(355..717)
                     /locus_tag="PK683_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH08431.1"
                     /translation="MVLDSNIIIYSANGNSFEELFREKNLYASVVSKIEVLGFHKITA
                     PQKENLEAFFDSIFILELTESVADEAISLRQKRNISLGDSIIAATALVHKLPLVTANV
                     SDFKWIEELEIINPLLSE"
     gene            complement(718..963)
                     /locus_tag="PK683_08010"
     CDS             complement(718..963)
                     /locus_tag="PK683_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08432.1"
                     /translation="MQTITIEIENPKDAEMILHLAERLNLRVLESGNTLGQMPNSRNA
                     LVHLRRLAEMGGLKNIIPDPVEWQKEIRRDRPLSGRE"
     gene            1158..1496
                     /locus_tag="PK683_08015"
     CDS             1158..1496
                     /locus_tag="PK683_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH08433.1"
                     /translation="MPKYSDRLDKVFYLLSDPTRRRVLEKLVKGPAAAGDLAGLFQMA
                     LPSFMQHLKILEKAKLVKSRKTGRVRIFEIIPGQMREAENWLSEQRNLWEKRLDQLDD
                     YLMQMKETEK"
     gene            1493..1987
                     /locus_tag="PK683_08020"
     CDS             1493..1987
                     /locus_tag="PK683_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH08434.1"
                     /translation="MNFTPDPKLDLVLERTVDVSPELVWKAWTTPEHLLKWFTPAPWK
                     TVECQIDLKPGGIFSTVMESPEGERHPNMGCYLEIVPNRKLVWTDALLPGFRPAKGRP
                     MNSFSFITAYIILEPHGTGGTKYTAVAVHSNEEEKEKHKAMGFYEGWGAALDQLVALM
                     KTMK"
BASE COUNT          763 a          504 c          495 g          660 t
ORIGIN      
        1 cgagttttct gataatgtct ccaggcttca gtcctgcctt agcagccggg gttccgggaa
       61 tcgccctgtt cacaaaaaca ccttccggct tatcctcaat cgaaacaccc atccaggctt
      121 ttacttcctg aaacccctga gattcctgtg ctttcagaca ggatgcaaaa aagaagagaa
      181 gaataaatac atgataaaat aatgattttt tcataaaacc tgacccatag agttattgat
      241 ccgtttcaga aaccgggata cagcctgatt ctgaaacgca tttttcataa atacatttca
      301 ttagactgcc ggtcaaacgg aaaagtattc tttttgattc gcatataggg cattttattc
      361 agataaaagc ggattaatga tctccaattc ttcaatccat ttaaaatcag agacattggc
      421 agttaccagc ggaagtttat gaacaagagc agtggccgca ataatggaat cgccaagcga
      481 aatattcctt ttctgtcgaa ggctgattgc ctcatctgca actgattcag ttaattcgag
      541 aatgaaaata gaatcaaaga atgcctccaa attctctttc tgaggtgcag taattttatg
      601 aaatccgagc acttctattt ttgagacgac agatgcatac agatttttct cacgaaataa
      661 ttcctcaaat gaatttccat ttgcagaata gatgataata ttgctgtcca gaaccattta
      721 ctctctgcct gacaacggtc tgtctctacg aatttccttc tgccattcga caggatctgg
      781 aatgatattt ttcaaaccgc ccatttctgc aagccgcctc aaatgcacca atgcatttct
      841 tgaatttggc atttgtccta aagtattccc gctttccaaa acccgtaaat tcaaacgctc
      901 tgctaaatga agaatcattt cggcatcttt gggattctca atttctatgg taatcgtctg
      961 catttaagtc tccataccat aactgtgaca ttcaaacaaa atcagtcaag cagaaagaat
     1021 agatctgttc cgcatctgat gattcttttt tgactgattt ctgcagacga tccggcagga
     1081 cggaacggaa tcagagagga tgcagagttt cgtcaaaaaa tatttgactt tcggatacgg
     1141 caagaatact taggtaaatg cctaagtatt cagacagact ggacaaagtt ttttatctgc
     1201 tttcggatcc gacgagaaga agggttcttg aaaaacttgt gaaggggcct gcagctgcgg
     1261 gagaccttgc gggtttattc cagatggctc tgccttcttt tatgcagcat ctgaagattc
     1321 tggaaaaagc aaagcttgtg aaatccagaa agaccggaag ggtccgcatc tttgaaatca
     1381 ttccgggaca gatgagagaa gcagaaaact ggctttcaga acagagaaat ctctgggaga
     1441 aaagactgga tcagctggat gattatctta tgcagatgaa ggagactgaa aaatgaattt
     1501 tacacccgat cccaaactgg atttagttct ggaaagaact gtggatgttt cgccggaact
     1561 cgtatggaag gcctggacaa ctccggagca tcttctgaaa tggtttacgc ctgccccctg
     1621 gaaaacagtg gaatgccaga ttgatctgaa accgggagga atattcagca cagtaatgga
     1681 atcccccgaa ggtgaaaggc atccgaatat gggatgttat ctggaaattg taccgaacag
     1741 aaaacttgta tggactgacg ctcttcttcc cggattcaga ccggcaaaag gtagaccgat
     1801 gaattcattt tctttcatta cagcgtatat catactggaa ccgcacggaa caggcggaac
     1861 aaagtacact gcagttgcag ttcattccaa cgaagaagaa aaagaaaaac acaaggcaat
     1921 gggattttat gaaggctggg gagctgctct cgatcagctt gttgcactga tgaaaacaat
     1981 gaagtaaaaa cataccctgc acctgatgtt tatccgtcag gggcaggaca gaaaattttt
     2041 gacaaaaacc ggcaacggaa cagattcaag aacatccgca ggaaatatat ctgacgggga
     2101 cagtacccta tcctatcaaa actgacagta taagaattta gaatgtttct catcggcaga
     2161 ctcagaccga attccgactg gaaaatcctt tgacagaaca tcggctgcag aaagttcccg
     2221 tattcacccc gacagaaata ggtgatttca cctatatacg ttatttataa tcaaacttac
     2281 aataaaattt atcattcaat caaaaaatat ttgactacta gaataaatta ttgaactgtt
     2341 gagattctgc ttaaatcatt cttatacgaa acactgtatt ttgccttgct cgatttggcg
     2401 actggagtgc atcttagagg ac
//
LOCUS       DAOQGJ010000439         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28206_length_2418_cov_2.956835, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000439 DAOQGJ010000000
VERSION     DAOQGJ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28206_length_2418_cov_2.956835"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..642
                     /locus_tag="PK683_08025"
     CDS             223..642
                     /locus_tag="PK683_08025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNH08435.1"
                     /translation="MIITPIQIRFNDMDPMRRVNNSVYSSYLELGRMDFCSRYISVDS
                     LEDIPFVLVRVELDILKSLRPGDKAEVHTCVSRIGKSSWDFSAVIINPLTNQVFAKAK
                     TVQVYFDYRLDAKADIPPAFREILESEMKQVQTRNNS"
     gene            775..1161
                     /locus_tag="PK683_08030"
     CDS             775..1161
                     /locus_tag="PK683_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08436.1"
                     /translation="MVKKLSLYFLLVFLNCADSSDSALKHTKPEAAERKVVSEEWSEE
                     QGRISWPEAMLKCTGMGMRLPTIMEMDKAFKSGETKKMNWKFGLYWTSEEKPKDPDRA
                     FAYYFDFGSADATRKSDVIDVRCRKK"
     gene            complement(1169..1423)
                     /locus_tag="PK683_08035"
     CDS             complement(1169..1423)
                     /locus_tag="PK683_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNH08437.1"
                     /translation="MTDREQEMDLARLYALMRPKNICICRAVSEKDLVNAVHNGCDTL
                     EKLIERTSASTKCGSCSFQVSAIFEREMNIIAAEKKQSED"
     gene            complement(1510..>2418)
                     /locus_tag="PK683_08040"
     CDS             complement(1510..>2418)
                     /locus_tag="PK683_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH08438.1"
                     /translation="YEVIKILLKKGADPKAKTAGYFGDVPSTPADYARDEGHVEIALK
                     LEGKNPASYRTTLHYFARKGDLEKVKEMIQKGANVNETEPRSNYTPLYYAGRYNHLDV
                     LKTLLAAGADPNKTFPAGVTVLRETVVRRFDRASRALIDAGAKADHEQTNGCGTGMTE
                     FSWAYNRGQYKLARYMIDKKAVTPSSKNLYRELFREKEDIKLAEYLIENQILPTHEDL
                     EVLKKLKKEWTEEKNQEILNRLIVLYEKTLEGKSAVAIKSSKQGAHLKKNISNKAQNR
                     NYVKGANEDNPNSHSLDSTIGKIRTE"
BASE COUNT          632 a          508 c          527 g          751 t
ORIGIN      
        1 cgggatttta ggcagtttca gagtcgccga gccagcttcc aaaccggcag ccatcttcat
       61 aaagcttggc gagggcggag cccttagcag tttcgaagtt tgcgaaaccg ccggtaggct
      121 cacttccaaa aacgccccac ctgcagccga aggcgtaagg tgaatgatta aaagtaaatt
      181 taagctctca ctgaaagtaa ccgaaaagca gagatataca atatgattat aacaccgata
      241 cagatcagat ttaatgatat ggacccgatg agaagggtga acaattctgt ctacagttcc
      301 tatctggaac tgggcaggat ggatttctgc tccaggtata tttctgtcga ttcactggaa
      361 gatattcctt ttgttcttgt gagagtcgag ctggacatac tgaaatcact caggccggga
      421 gataaggcag aggtgcatac ctgtgtttcc agaattggaa aatcctcctg ggatttttcg
      481 gcagttatta taaatccgct tacgaatcag gtgtttgcaa aagcaaaaac tgttcaggtc
      541 tattttgact acaggctgga tgcaaaagcg gatatccccc ctgcattcag ggaaattctg
      601 gaatcggaaa tgaagcaggt tcagacgagg aataattcct aagtatcttt attatccagt
      661 tctgaatgtc ctcggagttg gaactatgct cccaacgaat acggaatcag caaaaatgaa
      721 ttgaagattt gctgacaaga gcctttttat gatataacga ataaggagtt gattatggtt
      781 aaaaagttaa gtctgtattt tcttctcgta tttctcaact gtgctgacag ttcggattct
      841 gctctgaagc ataccaagcc tgaagcagcc gaaagaaaag ttgtttccga agagtggagc
      901 gaggagcagg gaaggatttc ctggcctgag gcaatgctga aatgtaccgg aatgggaatg
      961 aggcttccca caattatgga aatggacaaa gcattcaagt caggggaaac aaaaaagatg
     1021 aactggaagt ttggtctgta ctggacttct gaagagaagc ccaaagaccc tgatcgtgct
     1081 tttgcttact actttgattt tggaagtgca gatgcgactc gaaaatcaga tgtgattgat
     1141 gtgagatgcc gtaaaaaata aaaagatatc agtcctctga ctgttttttt tctgctgcta
     1201 tgatgttcat ctctctttca aagattgcac tgacctgaaa actgcaggag ccgcatttcg
     1261 tggaagcact tgttctttca atgagcttct ccagtgtatc acatccgtta tgaacagcat
     1321 tcaccagatc cttttcactc accgccctgc agatgcagat atttttggga cgcatcagtg
     1381 cataaagcct ggcaagatcc atttcctgtt ctctgtcagt catactttat aggacggtgt
     1441 tttccgggat attgtcaatg agatttctac gcttcaaaga caatatcttt cttcgttttg
     1501 agattccgtt tattctgtcc tgatttttcc tatggttgaa tccagggaat ggctgttcgg
     1561 attatcttca ttggctccct tcacatagtt cctgttttgg gctttattgg aaatattttt
     1621 cttaagatgg gctccttgtt ttgacgattt aattgcaacg gcagacttgc cttccaaagt
     1681 cttttcatac agaacgatga gccgattcag aatttcctga tttttctcct cggtccattc
     1741 cttcttgagt ttttttaaaa cttccaggtc ctcgtgtgtc ggaagtattt ggttttcaat
     1801 cagatattcc gccaatttta tatcttcctt ttctctgaaa agttcacgat aaagattttt
     1861 ggaggatggg gtgactgcct ttttatcaat catatatctg gcgagtttgt actgaccgcg
     1921 attatatgcc caactgaact cagtcattcc tgttccgcac ccattcgtct gttcatggtc
     1981 tgctttggca cctgcatcaa tcaatgctct ggacgccctg tcaaatcttc gtacaactgt
     2041 ttcccttaga acagtaacac ctgccgggaa agttttattt ggatcagctc cggcggccag
     2101 aagtgtcttc aatacgtcca aatgattgta acggccggca taataaagcg gtgtatagtt
     2161 agaacgcggc tcggtttcat ttacattcgc tcccttctga atcatttcct ttactttttc
     2221 aagatcacct tttctggcaa aatagtgcag ggttgttctg taggatgcag gatttttgcc
     2281 ttccagtttc aatgcaattt ccacatgacc ttcatccctt gcatagtctg caggggtaga
     2341 ggggacatct ccaaaatacc ccgctgtttt tgctttagga tctgctccct tttttagaag
     2401 gatttttatg acttcata
//
LOCUS       DAOQGJ010000440         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28240_length_2416_cov_3.605252, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000440 DAOQGJ010000000
VERSION     DAOQGJ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28240_length_2416_cov_3.605252"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PK683_08045"
     CDS             complement(<1..629)
                     /locus_tag="PK683_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08439.1"
                     /translation="MMKYICYITAMLFFSFSAVHSKEEHPYGFYKGVLLFSGTFGNPF
                     AFGGDLTDREKDIQKNLSLRSSLGLTPQKLYGVSQLPSGIGLPDTNFKNINSVRGVLE
                     YGISDSFGAGMSLSTLSMDSVNNRELPDYLKLSQNPNQLPSTYIDAFPRTAKFYSDQT
                     YSGVLSYHFLSKNRFDPFVYGEAGLVIFESSARQLKQNYLFESENRRGTG"
     gene            complement(719..1609)
                     /locus_tag="PK683_08050"
     CDS             complement(719..1609)
                     /locus_tag="PK683_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNH08440.1"
                     /translation="MHTHSHHDHDSYDHHGHSHHHHPTDSSSLKRLIYALIPVFLTFA
                     AEILGSWMSGSLSLLSDAGHLASDMTSLLISINAVILSRKKPSLDKPFGWKRIEVLAA
                     FVNSLLLIPVSIWIGVEAFHRILEPAEVHGHMIIWVGLAAIVGNFTGAAILHSYADYS
                     LNMKSAYLHLLSDLMGSIGVVTGGVIIHLTGWKIIDPIISGLIMLLILRIAFNLMRDT
                     AKSLLEFTPSNISFADVEKTVLEYSNALKILELKIWSLSSRENAASLHLQIADDFSKD
                     RLKETLKKKYRLSYVFIETN"
     gene            complement(1623..2111)
                     /locus_tag="PK683_08055"
     CDS             complement(1623..2111)
                     /locus_tag="PK683_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08441.1"
                     /translation="MKCGNCGGSVDLTDAVCGYCGQSLPVSVMSEESRIILSDFLQSM
                     EKILQANNNKYDPTIFIAFIMLVGFWGFATFRMTRFSGNPFFIGIASAVLGMFLFVSW
                     GFVISHMRQKSHTFAYENQVKGEIEIFLNRTGMTWQDFRQESMKILTDDSALYKIIVR
                     VN"
     gene            complement(2108..>2416)
                     /locus_tag="PK683_08060"
     CDS             complement(2108..>2416)
                     /locus_tag="PK683_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HNH08442.1"
                     /translation="YSSGSILAQKLILKYSDLGDLTKYVSRYFDTGSGGKREKESYIR
                     IAKDLDTESKYMLFFTDILEEAEAASSAGMTAMVLDRPGNKPQNEHAFPVLHGFYKFL
                     "
BASE COUNT          710 a          499 c          495 g          712 t
ORIGIN      
        1 cctgttcccc ttctgttttc tgattcgaat agataattct gcttcagctg tctggcggaa
       61 gattcaaata tgacaagtcc agcctctcca tatacgaagg gatcaaatct gttttttgag
      121 aggaaatgat aagaaagaac tcctgaataa gtctgatcgg agtaaaactt tgcagttctg
      181 ggaaacgcat caatataagt tgaaggcagc tgattcggat tttgagaaag tttcagatag
      241 tccggcagtt cccgattgtt gactgaatcc atactcaatg tacttaggga cattccagct
      301 ccaaaactgt cgctgattcc atattccaga acccctctga cggaattgat atttttaaaa
      361 tttgtatcag gaagccctat tcctgaaggg agttgagaaa ctccgtacag tttctgagga
      421 gtcaggccca atgaagatct gagcgaaaga tttttctgaa tatccttttc tctgtctgtc
      481 aaatctcctc caaatgcaaa gggatttccg aaggtacctg aaaaaaggag aactccttta
      541 taaaaaccgt atggatgttc ttcctttgaa tgaacggctg agaatgaaaa gaaaagcatt
      601 gctgtaatat aacatatata tttcatcatg aatgtccctg tcgtctggag gatctgaata
      661 ctcagaatgc atagtgtcca atttttgtcg acaaattatt tttaaatttt gtcgaaaatc
      721 agtttgtctc tatgaagaca taggaaaggc gatatttctt ttttaatgtt tctttaagtc
      781 tgtctttgga gaaatcgtct gcaatctgaa gatgcagaga ggctgcattc tctctggaag
      841 aaagtgacca gattttcagt tcaaggattt tgagagcatt cgaatactct aatacagtct
      901 tttccacatc ggcaaaactg atgtttgaag gcgtgaattc cagaagggac tttgccgtgt
      961 ctctcatcag attgaatgca atacgcagaa ttagaagcat aatcagcccg cttataatag
     1021 gatcaatgat tttccatcct gtgagatgaa tgatcactcc tcctgtcact actccgatcg
     1081 atcccatcaa atcggaaagc aggtgaagat aagcgctttt catattcagg ctgtaatctg
     1141 cataactgtg aagaattgct gcccctgtaa aatttcccac gattgcggca agacctaccc
     1201 agataatcat atggccgtga acctcagccg gttccagaat tctgtgaaat gcttcaacac
     1261 cgatccatat actgaccgga ataaggagaa gactgtttac aaatgcggcc agaacttcaa
     1321 ttcttttcca gccgaacggt ttatccaaag atggtttctt tcttgaaagt atgactgcat
     1381 tgatgctgat cagaaggctt gtcatatcag aagcaagatg acctgcatct gaaagcaggg
     1441 aaagacttcc gctcatccag ctccctagaa tttctgccgc aaatgtgaga aatacaggaa
     1501 tcagtgcgta aataagtctc ttcaatgatg aagaatctgt aggatggtga tgatggctgt
     1561 gtccgtgatg atcataactg tcatgatcgt gatgggaatg agtgtgcaat ggttcccctt
     1621 tttcagttca cacggacaat aattttatac agtgcagaat catcagtaag gattttcatc
     1681 gattcctgtc tgaaatcctg ccatgtcata ccggttcggt tcaggaagat ttcaatttct
     1741 cccttgacct ggttttcgta tgcaaatgta tgagacttct gtctcatatg ggaaattacg
     1801 aatccccagc tgacaaaaag gaacatccca agcactgctg atgcaattcc gataaaaaaa
     1861 ggattgccag agaatcttgt cattcggaaa gtcgcaaatc cccagaatcc gacaagcatg
     1921 ataaatgcaa taaaaatggt aggatcgtat ttattattgt ttgcctgaag tattttttcc
     1981 atactctgaa gaaagtctga cagaataatc cgtgactctt ccgacattac tgaaacggga
     2041 agcgactgac cgcagtagcc gcacactgca tctgtcagat caacagagcc tccgcagttt
     2101 ccgcatttca taaaaattta tagaatccat gcagtacggg aaaagcgtgt tcattctgtg
     2161 gtttatttcc cggcctgtcc agtaccatcg ctgtcatacc tgcagaagat gctgcctctg
     2221 cttcttcgag gatatccgtg aagaaaagca tatatttaga ctcagtatcc agatctttcg
     2281 caattctgat ataactttct ttttctcttt tgccgcctga accagtgtca aagtatcggc
     2341 ttacatattt tgtaagatca cccaggtcgg aatatttcaa aatcagtttc tgtgcaagaa
     2401 tgcttccgga ggagta
//
LOCUS       DAOQGJ010000441         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28241_length_2416_cov_3.383312, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000441 DAOQGJ010000000
VERSION     DAOQGJ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28241_length_2416_cov_3.383312"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..2299)
                     /locus_tag="PK683_08065"
     CDS             complement(227..2299)
                     /locus_tag="PK683_08065"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08443.1"
                     /translation="MKKLQEKFRLQSISTKLLISQILLFGIAVLLLMLLSTKITRIFF
                     LTSEEENLIQTNRQVLDMVEMYDVSLKQTAARIANIFRLTGGNRGTENVTEQEIDDFT
                     VKTGAAATIFRKQGDDFLRIKTSILKQDRTRAVGTMLDRKHPAYRHLLEGKPFFGKAK
                     LFGKDYMTFYEPVLEGGTYTGARFIGIDITKDMEDLKQKIRRIKIGETGYSYVMDSSE
                     EEKGILIVHPAKEGANVLNSRGSDGTDFVGAMLNNKSGVIYYSWINEERGEIFSREKV
                     AVYDRYPEWNWIIVSGSYVSEILQDGKIMMTSLIIGLCIFFLLLMAVQRYIAVTLVKE
                     PLQNLTDVMDRISGGDLTLASESAMRNSKDETDILQLKLIGFASKVRDAIGRMMNASI
                     HLNDSAGRMTRTSEGFSSSAAEQSASAMQITSSLSLISSSMDRIASLSSEQNGNLEKM
                     FETGASLSGIIEKMEKTMRESFDLSIKVSGNAESSSASLLEMKESIERIMKSSNSITG
                     IVRIIRDISKQINLLSLNAAIEAARAGDMGKGFAVVADEVTKLADETSRSIRNIDSLI
                     QGNKEDILNGIEGVNSSIKKISLMIEDIKKIKNISEELSGQMAVQVSINKDVGTAAGK
                     VGLGANEIFHATHDQKAAINEILHAMSEMNTAAQFNSSGAEELALGARQTENLADEIK
                     KDIGFFKI"
BASE COUNT          561 a          560 c          487 g          808 t
ORIGIN      
        1 ttccctgaca tcaatcagct gataaagagt gcccgaattt ctcagctgtt tgaactcttt
       61 gactgtaatc tcttttactt cattaggatt tgtcattatt ctccactttt ttatgccgca
      121 gtctgcagga aagaaaaaat ttccattaaa ttggagagat tattcgaaaa aacggaaatt
      181 gatcgggcgg gggaaaattc ctgaaattca acaatctgcg cctccctcag attttgaaaa
      241 agcctatgtc ctttttgatt tcgtctgcca ggttttctgt ctgtcttgcc cccagtgcca
      301 gttcttctgc tcctgaggaa ttgaactgtg cagcggtgtt catctctgac attgcgtgaa
      361 ggatttcgtt tattgcagct ttctggtcgt gcgttgcgtg gaaaatttca ttcgcaccga
      421 gtcccacctt tccggctgca gttcctacat ccttgttgat gctgacctgc actgccatct
      481 gtccagaaag ctcttctgat atatttttaa tctttttaat atcttctatc attaagctga
      541 tcttttttat ggaactgttt actccttcaa tcccgttcag aatgtcttcc ttgtttccct
      601 gtataagact gtctatgttt ctgatgcttc gggaagtttc atctgcaagt ttcgtcactt
      661 catctgccac cactgcaaag ccctttccca tatcccctgc acgggccgct tcaatggcgg
      721 cattcagcga aagaagattg atctgtttgg atatgtcacg gataattctt acaatgccag
      781 tgatggaatt ggagcttttc attattcttt caatgctttc tttcatttcg agaagtgatg
      841 ctgaactgga ttccgcattt cctgaaacct tgatggaaag atcgaatgat tccctcattg
      901 ttttttccat tttttcaatg atgccggaaa gtgaagctcc tgtttcaaac atcttttcca
      961 gatttccatt ctgttctgaa gaaagagatg cgattctgtc catactcgat gagatgagag
     1021 acagagagga tgtgatctgc attgccgatg cagactgttc ggcggcactg gaagaaaaac
     1081 cttcggacgt tcttgtcatt cttcctgcag agtcgttcag gtgaatggaa gcattcatca
     1141 ttctgcctat ggcatctctg acctttgacg caaagccgat gagtttcagc tgaagaatat
     1201 ctgtttcgtc tttggagttg cgcatggcag attcagatgc cagggtcagg tcgcctcctg
     1261 aaatcctgtc cattacatcc gtaaggttct gaagaggttc ttttacaaga gtgactgcaa
     1321 tataacgctg cactgccatc agaagcagga agaagataca aagacctata atcagcgaag
     1381 tcatcattat cttgccgtcc tgcaggattt cactgacata acttccgctc acgataatcc
     1441 agttccattc aggatacctg tcgtagacag ccactttctc tctgctgaaa atttctcctc
     1501 tttcttcatt gatccaggaa taatagatga ctccggattt attgttcagc atagctccga
     1561 caaaatcggt tccgtctgaa cctctgctgt tcagaacatt tgccccttct tttgcagggt
     1621 gaacaatcag aattcccttc tcttcttctg acgagtccat tacataggag tatcctgttt
     1681 caccgatttt aatcctgcgt attttctgct tcaggtcttc catatctttt gtaatgtcga
     1741 tgccgatgaa gcgcgcgcct gtgtatgttc cgccctccag caccggttca taaaaagtca
     1801 tataatcttt tccgaagagt ttcgcctttc cgaaaaaagg ctttccttcc agcaggtgcc
     1861 tgtacgcagg atgttttctg tcgagcattg tgcctaccgc acgggttctg tcctgtttca
     1921 gtatggaagt ctttatcctg agaaaatcgt ctccctgttt tctgaatatt gtggcggcag
     1981 ctcctgtctt gacagtaaaa tcgtcaattt cctgctctgt gacattttcg gttcccctgt
     2041 ttcctccggt aagccggaat atgttggcaa tccttgctgc ggtctgtttc aggcttacat
     2101 cgtacatttc caccatatcc agaacctgtc tgtttgtctg tatcagattc tcctcttccg
     2161 atgtaagaaa aaagattctc gtaatttttg tagagaggag catcagaagc aggacggcaa
     2221 tgccgaacag cagaatctgt gaaatgagaa gttttgtgct tatgctttga agtctgaatt
     2281 tttcctgcag ttttttcaat gtccatccgt tccagttcaa aagaattcag gcagagtgca
     2341 taagattgac tgcatttatt tacgctctca tttgttcaga tcgtcatcga tgcctgtttt
     2401 tttctgtatt tacatt
//
LOCUS       DAOQGJ010000442         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28255_length_2415_cov_3.689831, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000442 DAOQGJ010000000
VERSION     DAOQGJ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28255_length_2415_cov_3.689831"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            584..889
                     /locus_tag="PK683_08070"
     CDS             584..889
                     /locus_tag="PK683_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08444.1"
                     /translation="MKLFKLFTILIVSLSLSFSAGLFAGDKKEKDKKEDKADKKDHKD
                     HKKDGKDGGVSCEKKCGHTHSACEMKNADFPKGSKERKAHNEKCQSDYKSCKSSCKT"
     gene            complement(1028..>2415)
                     /locus_tag="PK683_08075"
     CDS             complement(1028..>2415)
                     /locus_tag="PK683_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08445.1"
                     /translation="YRSALKKKVSPDAKRMLITGSSLAAYSFDAEKISEYLKERSGQK
                     ISTDILSYAGMTPLDMYLQKDKFLALKPDIIVIPVNFIDFRVHRAYILNPSGTNFDAD
                     ENILLKDAVDFYEAQQSKYLFPGQALTETLGKVPFEQSAAYLNSFVFLFYRYREIYLE
                     NLRNLYNHRFGRNTSYHAYQGAEIGGGISHLGWTGRHFSFIMKKYMQKDGFYIQIVPE
                     ILTSGPLKTVFRSKDSVQEEVFSSPGWKKILLKEDFHPETEISAELSSVWVPFLAKED
                     RFDYAREEMGVRLQQTFGLEAPKQNLHSKREERSEDRRYEKMSRENYREYFYYRLLAD
                     PEKRPGIGYLSALKNAKQRIAKEKFKPILQFRYLQKTAEYYNSQNIKVFIINNPENPL
                     SLEWYSESEWYSEHLKFLRSLENRNVHFIDIHGMLKEQDFSDFHHFTYPAMMQMNSVY
                     GDIILDRLNRQ"
BASE COUNT          704 a          475 c          463 g          773 t
ORIGIN      
        1 gccggacagg cccaaagggg tttcgcaaac ttggaaactg ctgcaaaccc ctttggaatc
       61 cccgcttttt actcacaatt agtcatcgga aaatataatt tcaatcaata atcaattgac
      121 tctatgaaaa tttaattaga aatttgagtt ttagtatata taataaatat tatatatggg
      181 tgaagaattt ctgtcttcaa ggtgtttttg cggaacggca gctaattttt ttctgtattt
      241 ttaaatgaac atttactgaa ttttaatctg aattctctca ttgctcagac aggaatacag
      301 tgcctaaagc ctttattttt actgaagcat ctgaactcgg atttccagac tcgcaggaaa
      361 tgaatctgca tctgcagtac gccttgcgga ctgctgaaaa tcaaaaatgg aatggatgtc
      421 tttctgcagt aatttccgaa ccagtactgt tttaaaagaa cggaagatga gtgaattctt
      481 taaaaaaata ctgcttggga taagaatatt ttttaaagtt ccttgacagt ttttctgtca
      541 gttcagtcta ataaatacaa tctagaatat tggagatttt ctgatgaaac tttttaaact
      601 ctttacgatc ctgatcgtat cactttcttt atctttttct gcaggcctgt ttgcaggtga
      661 caaaaaagaa aaagacaaaa aagaagataa ggcagataaa aaagatcaca aagaccacaa
      721 gaaagacggt aaagacggcg gtgtaagctg tgaaaaaaaa tgcggccaca ctcacagtgc
      781 atgtgaaatg aagaacgcgg attttccaaa aggatcaaag gaaagaaaag cgcataatga
      841 aaaatgccag tctgactaca aatcctgcaa gtcttcctgc aagacctgat tctctgtaaa
      901 attgactcag tctgctccct gcagactgag ttttattctt ataaaatcca actcacccca
      961 tagctggagc attttttagc tgctgtagct atgatatttc tcatagaagc ttgaatggtt
     1021 tttctgttca ttgtctgttt agcctgtcca atatgatatc accgtaaaca gaattcatct
     1081 gcatcattgc aggatatgtg aagtgatgga aatctgaaaa atcctgttct ttaagcattc
     1141 catgaatatc tatgaaatgt acattcctgt tttccagtga cctgagaaac ttgagatgct
     1201 cagagtacca ttccgactct gaataccact ctagagagag cggattttca ggattattta
     1261 taataaatac ttttatattt tgggaattgt agtattctgc ggttttctga agatacctga
     1321 actgcagaat aggcttgaac ttctccttcg caattctctg tttggcattc ttcaatgcag
     1381 acagatatcc gatgcctggt cttttttcgg gatctgccag aagcctgtag taaaaatatt
     1441 ctctgtaatt ttctctggac atcttttcat atcggcggtc ctcgcttctt tcctctcttt
     1501 tggaatggag attctgcttc ggtgcttcca gtccgaaagt ctgctggagc ctgactccca
     1561 tctcttccct ggcataatca aatctgtctt cttttgcaag gaagggcacc cataccgaag
     1621 aaagttctgc actgatttcc gtttcaggat gaaaatcttc cttgagcaga atttttttcc
     1681 acccaggaga actgaagacc tcttcctgta cagagtcttt ggatctgaag actgtcttta
     1741 atggccccga tgtcagaatt tcaggcacaa tctgtatata aaaaccgtcc ttctgcatat
     1801 attttttcat tataaaactg aaatgtctgc cagtccatcc cagatgagag atgccccctc
     1861 cgatttctgc accctgatag gcatgataac ttgtgtttct tccaaaccgg tgattgtaaa
     1921 gatttcttaa gttctcaaga taaatttctc tgtatctgta aaaaagaaag acaaaactgt
     1981 tcagataggc agcggactgt tcaaacggaa cttttccgag tgtttctgtc agtgcctgtc
     2041 ccggaaagag atattttgac tgctgtgcct cataaaaatc cactgcatcc ttcagaagaa
     2101 tattttcatc tgcatcgaag ttggttccgg agggattgag tatatatgcc ctgtgtactc
     2161 tgaagtcaat gaaattgacc ggaattacga tgatgtccgg tttaagtgca agaaacttgt
     2221 ctttctgaag atacatatca agaggagtca ttcctgcata agaaagaatg tccgtgctga
     2281 ttttctgtcc gcttctttct ttaaggtatt cggaaatctt ctccgcatcg aaagagtagg
     2341 cggccaggct gcttccggtt atgagcatcc tttttgcatc aggagaaact ttttttttta
     2401 atgcagatct gtatt
//
LOCUS       DAOQGJ010000443         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28321_length_2412_cov_4.056428, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000443 DAOQGJ010000000
VERSION     DAOQGJ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28321_length_2412_cov_4.056428"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="PK683_08080"
     CDS             complement(<1..1540)
                     /locus_tag="PK683_08080"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH08446.1"
                     /translation="MLKRPELMKLPLTPLALLMISYSISAQNMLHFSEYQGSMNWNDA
                     DQKCRGIRMRLPSLQELDEGKNTAWLQNGSAPEFWTSEKVSEQSVRVFRLYLNVRDRL
                     TVMEPAESIPSAGKGVICVSDAPAGSIEAELFFQKERIQRIREELSKNIDNKIFMEKK
                     VIQFAKLKNRTEMVSLSKIRETDKVQAHYIIFRSSEGNVFAEEFLYRKTGQWHIALRY
                     VFDAQGRLFYRNMSGTAGLGGDNFDEGYVDHYYINDRLIHSEKGCYGQRMDCKVLHSL
                     RFKRDTEFLSFLKKRKFPKAVLQKQKDSYKWNRVLKESVKYGDLKKMHRALEKGADVN
                     LRDTGLASPVFSCGYYIRKKEYGTELLWNSEIVKVLLENGADVKTKDLNGDTPLAKCL
                     SIGNYYNDSEHSEKLKNLTSLFIKYKADINARDREGKSPIFYGQIIKFLIDNGADVNA
                     KDREGNTPLHTVFSPDSAKILIENGALPDAKNLLGETPLILYCGNDEKNGAGLGNTEK
                     AELLL"
     gene            complement(1543..2040)
                     /locus_tag="PK683_08085"
     CDS             complement(1543..2040)
                     /locus_tag="PK683_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08447.1"
                     /translation="MKLRIIFTALVLVFALQTDAKKGDPEKFSTKNIEVVLKDENGFS
                     HFIVFSKIRNSEGSKSVYTADFWVNDQVSIVGYEVWSIMFRTDEENYAYRISRYDRGG
                     SYDSAKVLYFEMPEEELKLIADAKNIKVRMIMKGEKLGAFPFQDYSAVKENELRRSMK
                     EILKY"
     gene            2197..>2412
                     /locus_tag="PK683_08090"
     CDS             2197..>2412
                     /locus_tag="PK683_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08448.1"
                     /translation="MFAYELHRPASQIRNYIQNYHFIQGSSLSVHQRTSCLSNVSLII
                     NYGDEFHTVTDDNIFHKYANGIYTAGMF"
BASE COUNT          656 a          554 c          412 g          790 t
ORIGIN      
        1 ttaaaagcag ttcagccttt tcagtgttcc cgagacctgc cccgtttttt tcatcgtttc
       61 cgcagtaaag aatcaaaggg gtttctccca gaaggttttt tgcatcagga agagcaccgt
      121 tttcaattaa aattttcgca gagtccggac tgaaaacagt gtgcagagga gtgtttcctt
      181 ccctgtcttt tgcattgaca tctgctccgt tatctataag aaattttata atctgaccgt
      241 aaaagatcgg agactttcct tccctgtctc ttgcattgat gtcagcttta tatttaataa
      301 ataatgatgt gagatttttc agtttttcag aatgttcact gtcattgtaa taatttccga
      361 ttgaaaggca ttttgccaaa ggagtatctc cgttcagatc tttggttttt acatccgctc
      421 cgttttccag aaggactttg actatttcag aattccagag cagttcagta ccgtattctt
      481 ttttcctgat ataataaccg caggaaaaga caggagaagc aaggcccgtg tccctgagat
      541 tgacgtctgc tcccttctcc aaagcccgat gcattttttt cagatcaccg tatttgaccg
      601 attctttcag aactctgttc catttataag agtctttctg tttctgcaga acagcttttg
      661 gaaattttct cttttttaaa aatgacagaa attctgtatc ccgtttgaac ctcagagaat
      721 gcagaacttt acagtccatt ctctgcccgt aacagccttt ttcagaatga atcagtctgt
      781 cattgatata ataatgatca acatatccct catcaaaatt gtcgcctcca agacctgccg
      841 tgccggacat attcctgtaa aacagtctgc cctgcgcatc gaagacatat ctcagtgcaa
      901 tatgccactg tcctgtcttt cggtataaaa actcttctgc aaacacattt ccttcagaac
      961 tcctgaagat gatataatgg gcctgaacct tatctgtttc tctgattttt gaaagactga
     1021 ccatctcagt tctgtttttg agctttgcaa actgaatcac cttcttttcc ataaatattt
     1081 tattatctat atttttagaa agttcctctc tgattctctg aatcctctct ttctgaaaaa
     1141 acagttccgc ttctatactg cctgcaggag catctgaaac acagatgact cccttgcctg
     1201 ccgaaggaat cgactctgcg ggttccatca ctgtcagtct gtccctgaca ttcaaataca
     1261 gacgaaagac tctgacagac tgctcactga ctttctccga agtccaaaac tccggagcag
     1321 atccgttctg gagccaggct gtattctttc cctcatccag ttcctgcaga gaaggaagtc
     1381 tcatccgaat gcctctgcat ttctgatctg catcattcca gttcatagat ccctgatact
     1441 cactgaaatg gagcatattc tgtgcactta tactataaga aatcatgagc agcgcaagcg
     1501 gggtaagcgg cagtttcatc agctcaggcc ttttcagcat actcagtatt tcagtatttc
     1561 cttcatcgaa cgtctgagtt cattttcctt cactgccgaa taatcttgga acggaaaagc
     1621 accgagtttt tctcctttca taatcattct gactttaatg ttctttgcat ctgcaatcag
     1681 tttcaattcc tcttcaggca tctcaaaata aagaaccttt gcggaatcat aggaaccgcc
     1741 tctgtcatat ctggaaattc tgtatgcata gttttcttca tcagtgcgga acataatgct
     1801 ccagacttca taacctacga tagatacctg atcattgacc cagaaatccg cagtatatac
     1861 tgatttagat ccttctgaat tcctgatttt tgagaataca ataaaatgac tgaatccgtt
     1921 ttcatccttc agtacaactt caatattctt cgtggagaac ttctccggat ctcctttctt
     1981 tgcatctgtc tgcaaagcaa aaaccagcac caatgccgta aaaatgattc tgagtttcat
     2041 ctatgtctcc ttattatctg gaatgattat tcttagcatt caaatatgct ttcgtattgt
     2101 aaaaaacgga caggtgcgag tcttttttca gcaattctga aaaatatatg ctgaagaact
     2161 gtttttattt tacaggagca tcagcccgga gcttatatgt ttgcctatga actgcacaga
     2221 ccggccagtc aaatccggaa ttatattcag aattatcatt tcatccaagg cagcagcctc
     2281 tctgttcacc agagaacatc ctgcctgagc aatgtatctc ttataataaa ttacggagat
     2341 gaatttcata cagtcacaga tgataatatt tttcataaat atgcaaatgg aatttacact
     2401 gcaggaatgt tt
//
LOCUS       DAOQGJ010000444         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28330_length_2412_cov_2.263046, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000444 DAOQGJ010000000
VERSION     DAOQGJ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28330_length_2412_cov_2.263046"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2412
                     /gene="mfd"
                     /locus_tag="PK683_08095"
     CDS             <1..>2412
                     /gene="mfd"
                     /locus_tag="PK683_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNH08449.1"
                     /translation="SFLPPEKLISKGAERDILFNSSAICFRPVFKDLVSAGEMSLDIS
                     SGTQFRGKIREAREKTAEILSDEESRVILLTSFTAQAERLTGLFKEQNPQYLNSGEEP
                     GIIELSSENRFQIIVSDLRSGFILNEKKIHIFTERDIFGRDYKRKNKFKKTSSRAIDS
                     FIDLREGDYIVHIQHGIGRFKRIEKVEAGGKVRDFIRLEYGDENTLFIPLDQISQIQR
                     YAGGLEHPPLDTLGKGLWKKKLERAKKSVSRLAEELLIAYANRMKLQGYAFPPDSIWQ
                     EEFEAEFEYEETPDQISAIEAVKNDLESPRPMDRLICGDVGYGKTEVAIRAAFKVVMS
                     GKKVLFIAPTTILALQHYNTLRSRYENYPIAVDMVSRFRTAGEVRDIISRFNENKIDI
                     LVGTHALLNSKTDMKNLGLLIIDEEQRFGVNQKESFKKYKNMVDVLTLSATPIPRTLH
                     MSLTGIRDLSIIETPPKNRQSVETYVMDEDEEIIAHAVRRELERGGQVFYLHNRVASI
                     ETEADYLRNLIPEVSVGILHGQLTEEEVEETIMDFTARKYDVLVTTTIIESGIDMPNV
                     NTIMVKRADMFGLSQLYQIRGRVGRAGRKAYAYLFYPKDKSLTEQAEKRLSTIHEYQE
                     LGSGFKVAMRDLEIRGAGNLLGKEQSGNIMEIGFELYVKLLNEEVSRLKNEAVEVEVR
                     TQINLNTDFYIPEDYISDTRQKVEFYKKFEAAADLDEIEELSREMRDRFGEFPESVKT
                     FIMLEKIRSVSSGLGFESITEDEGNIRMKAGQHFTGDPERMIQIISDPSSRLSMIPRQ
                     PNVLR"
BASE COUNT          741 a          465 c          605 g          601 t
ORIGIN      
        1 tttcttttct tcctcctgaa aaactcatat caaagggagc tgaaagagat attcttttta
       61 attcttctgc gatctgtttt aggccggtct tcaaggatct tgtttctgca ggagaaatgt
      121 ctctggatat cagttcagga actcagttcc gaggaaagat ccgggaggca agggaaaaaa
      181 ctgcagaaat actttcggat gaggaatcga gagtcattct tcttacttcc ttcactgcgc
      241 aggcagaaag acttaccggg cttttcaagg agcagaatcc gcagtatctg aattcaggag
      301 aggaaccggg aatcattgaa ctttcttcag agaacagatt tcagataatc gtatccgatt
      361 taagaagcgg atttatttta aatgaaaaga aaatccatat atttactgaa agggatatat
      421 tcggaagaga ttataaaaga aagaacaagt tcaagaaaac ttcttcgagg gcgatagaca
      481 gtttcattga tctgcgggaa ggcgattata ttgtgcatat acagcacgga atcggcaggt
      541 tcaagcggat tgaaaaagtg gaggcaggcg gaaaagtaag agattttatc cgtctcgaat
      601 acggagatga aaatacgctt ttcattcctc ttgaccagat ttcccagata cagaggtatg
      661 caggcggtct ggaacatcct cctctggaca ccctcggaaa aggactctgg aagaaaaaac
      721 ttgaaagagc caaaaaaagc gtcagccgtc ttgcagaaga acttctgatt gcatatgcca
      781 acaggatgaa gcttcaggga tatgctttcc cgcctgacag catctggcag gaagagtttg
      841 aggcagagtt tgaatatgag gagacgccgg atcagatttc tgcaattgaa gctgtgaaga
      901 atgatctgga atcgcccagg ccgatggaca gactgatctg cggagatgtg ggctacggca
      961 agactgaagt ggccatccgc gctgccttca aggtagtgat gtccggaaag aaagtgcttt
     1021 tcattgcacc gactacaatt cttgctctgc agcattacaa tactctgaga agcaggtatg
     1081 agaattatcc gattgcagtg gatatggttt cgagattcag aactgcagga gaagtcaggg
     1141 atattatatc ccgttttaat gaaaataaaa tagacatact tgtcggaact catgcccttc
     1201 tgaactcgaa gactgatatg aagaatcttg ggcttctcat catcgacgag gaacagaggt
     1261 tcggagtcaa tcagaaggag tctttcaaga aatacaagaa tatggtggat gttctgactc
     1321 tttctgcaac accgattccc agaacccttc atatgtctct caccggaatc cgggatcttt
     1381 ccattattga gactcctccg aagaacagac agagcgtgga aacatacgtg atggacgagg
     1441 atgaggaaat cattgctcat gcagtcagaa gagaacttga aagaggaggg caggtattct
     1501 atctgcacaa cagggttgcc tcgatcgaaa cggaagcgga ttatctcaga aatcttatcc
     1561 cggaagtctc tgtaggcatc ctgcacggtc agcttacgga agaggaagtc gaggaaacta
     1621 ttatggactt cactgccagg aagtatgatg tgcttgtaac gacgactatc attgagtccg
     1681 gaatcgatat gcccaacgtc aatacgatta tggtaaaacg cgctgatatg ttcggacttt
     1741 ctcagcttta ccagatcagg ggacgcgtcg ggcgtgcagg aagaaaggca tacgcatatc
     1801 ttttttatcc gaaggacaaa tcgcttacag aacaggcgga gaagaggctt tccacaattc
     1861 atgaatatca ggaactgggt tcaggattca aggtggcaat gagagatctt gaaatcaggg
     1921 gagcaggaaa tcttctcgga aaggaacaga gcgggaacat tatggagatc ggttttgaac
     1981 tgtatgtaaa gcttttgaat gaagaagtca gccgtctcaa gaatgaagcc gtggaagtgg
     2041 aagtgagaac ccagatcaat ctgaatacgg acttttacat tcctgaagat tacatttccg
     2101 atacaagaca gaaggtcgaa ttttacaaga agtttgaagc ggcagccgac cttgatgaaa
     2161 tagaggaact ttcccgcgag atgagagaca gattcggaga gtttcctgaa tctgtaaaaa
     2221 cttttattat gctcgaaaag atacgttctg tcagttcagg tctcggattc gaatccatca
     2281 ctgaagatga gggaaatatc cgaatgaagg caggtcagca ttttacggga gatccggaaa
     2341 ggatgattca gatcatatcc gatccttctt cgagactgag catgattcct agacagccca
     2401 atgtgcttcg gt
//
LOCUS       DAOQGJ010000445         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28341_length_2411_cov_2.457555, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000445 DAOQGJ010000000
VERSION     DAOQGJ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28341_length_2411_cov_2.457555"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>2411
                     /locus_tag="PK683_08100"
     CDS             52..>2411
                     /locus_tag="PK683_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08450.1"
                     /translation="MADKIQKLSENTVSVIKFAACIGARFYLDNLSIICGKPESEVID
                     DLKEAIDEGMILVGGSSAKFAHDRVREAAYSLISEEGKIQYHYEIGKILLEKTAPELL
                     DDRIFSILGQLNLGKILAANDEEKIHLVSLNLKAGRKAKASAAYEGSLNFLRDGAELL
                     NAESWNENYSLTLELYTELAECEYLAGHFEEADRLYEIILSNAKELLDKIPVLFIQLR
                     QKTTQIKTAEAFQIGFSVLRDLGLKMPDPDNPEEAQGAFFSQLEEYKSLLGSRQISDL
                     INLPEISDRRMQYALEMVTNLGDVAIVQRQDLLGLMSILGVNLSLQYGNTAVSSISYV
                     MWGVINCLGFKDYKSGYEFGQLSLKLNKERFPDSLILGRILAFYTWNINHWQKNIKED
                     HPLAKEGLEITLNNSDLVYASYFAPNPMMVSFFRGEPLDDVIENGKKNNIFAEKYRQV
                     LSSMLGSSTLMTSLALQGKTAGHCSLNSAEYGFDEEKFIQSFSLYGQPMIYFYLRKFQ
                     LFCLYWKFNEAYSILPELEKYFPNMPAHIAYSEYYFFKTFMLLNISPENMKEEDKKNR
                     QDKLEEGFSLFKMWSEHCEDNFLHKYLLLHAEKARVTGEYLAAMDLYDDAAESAAKYE
                     YTNDLAFISEAAARYYISKGKSKLAQPYLRDAHYFYGKWGASAKQADLEQKFYEILKS
                     SLAKRAVFSSTVSSDAAAKLAATTAGSSFLDLNTVVKASQAISGEIQLGRLLERMMKI
                     LFENAGAEKGSFIVRDKGKWIVQAEGNSKGKIEVLQATSLNGSAKDG"
BASE COUNT          740 a          434 c          560 g          677 t
ORIGIN      
        1 gatatgcaga aaatcctgtc agtgaatatt tccgacaata tagttgacct gatggcggat
       61 aagattcaga agctttctga aaacacggta tccgtaataa aatttgccgc ctgcatcggt
      121 gctagatttt atctcgacaa tctttcgatt atctgcggca agcctgaatc tgaagtgata
      181 gatgatctga aagaggcgat tgacgaggga atgattcttg tcggagggag ttctgccaag
      241 tttgctcatg accgtgtaag ggaagctgca tactcgctga tcagtgaaga aggaaaaata
      301 caatatcatt atgaaattgg gaaaattctt ctggaaaaaa cagcccctga actgctggac
      361 gacaggattt tcagcattct cggtcagctg aatctgggca aaatccttgc agcgaatgat
      421 gaggaaaaga ttcatttagt ttccttgaac ctcaaagccg gacggaaggc aaaggcttct
      481 gcagcgtatg agggatcatt gaatttcctc agggatggag cagaacttct caatgcagaa
      541 agctggaatg aaaactattc ccttactttg gaactttata ctgaacttgc cgaatgtgaa
      601 taccttgcag gacattttga ggaggctgac agactgtatg aaataattct ctcaaatgca
      661 aaggaacttc ttgataagat tccggtattg ttcatacagc ttcgtcagaa aactacacag
      721 ataaaaacag ctgaagcatt tcagataggg ttttcagttt tacgggatct gggtcttaag
      781 atgcccgatc cggataatcc agaagaagca cagggcgcat ttttttcgca gctggaagaa
      841 tacaaaagtc ttctagggtc cagacagatt tctgatctga ttaatctgcc tgaaatatca
      901 gaccgtcgaa tgcagtatgc tttggaaatg gtgacaaacc tgggggatgt tgcgatagtt
      961 caaaggcagg atctcttagg cctgatgtca atactgggag tgaatctgtc cctccagtat
     1021 gggaacaccg ctgtttcttc tatatcatac gtaatgtggg gagtcattaa ctgtctgggg
     1081 ttcaaggact ataaaagcgg atatgaattt ggacagttga gtttaaaact gaacaaagaa
     1141 cgttttcctg attcattgat tcttggccgg attcttgcct tttatacttg gaacatcaac
     1201 cattggcaga agaatataaa ggaagaccat cctctggcta aagagggttt agaaatcact
     1261 ctgaacaaca gtgatctggt ttatgcatcg tactttgctc ctaatcctat gatggtttct
     1321 ttttttcgag gagaacccct cgatgatgtg attgaaaatg ggaaaaagaa caatatattt
     1381 gcggagaaat acaggcaggt tttgtcatca atgctgggca gcagtacttt aatgacgtca
     1441 cttgcactgc aggggaaaac agccggtcac tgctctctga attcagctga atatgggttt
     1501 gatgaagaga aattcattca gagcttttct ctctatggtc agccgatgat atatttttat
     1561 ctgcgaaaat ttcagctttt ctgtctttac tggaagttta atgaagcata ctcaatttta
     1621 cccgaactgg aaaaatattt tccgaatatg cctgcccata ttgcttattc agagtattat
     1681 tttttcaaaa cgtttatgct gttaaacatc agtcctgaga atatgaagga agaagataaa
     1741 aaaaatcggc aggataaact tgaggaagga ttcagtcttt ttaaaatgtg gtcagaacac
     1801 tgtgaggaca attttcttca taagtatctt cttctgcatg cagaaaaggc cagggtgaca
     1861 ggcgaatatc tggcagcaat ggatctctat gatgacgcag cggaaagtgc tgcaaaatat
     1921 gaatatacca atgaccttgc ctttatatcc gaggcagcag caagatacta tatctctaaa
     1981 ggcaaatcta agctggcaca gccctatctg cgtgatgctc attattttta tggaaaatgg
     2041 ggagcatctg caaagcaggc tgatcttgag cagaagtttt atgagatatt aaaaagctct
     2101 ctggcaaaac gagcagtttt cagttcaaca gtcagttccg atgctgcagc taaactcgca
     2161 gcaacaacag ctggatcgag ttttcttgat ttgaatacgg ttgtcaaagc gtctcaggca
     2221 atatccggtg aaattcagct gggaagactt ttggagagaa tgatgaaaat tctttttgaa
     2281 aatgccggag cagaaaaagg ttcatttatt gtccgtgaca aagggaaatg gattgtacag
     2341 gcagaaggaa attcaaaggg aaaaattgaa gttctgcagg caacgtcatt gaatggatcc
     2401 gcaaaggatg g
//
LOCUS       DAOQGJ010000446         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28358_length_2410_cov_3.458599, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000446 DAOQGJ010000000
VERSION     DAOQGJ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28358_length_2410_cov_3.458599"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..725)
                     /locus_tag="PK683_08105"
     CDS             complement(177..725)
                     /locus_tag="PK683_08105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNH08451.1"
                     /translation="MKFDKSIKISLGLVALLWIITIADFLIPLDFTRLGIYPRTFFGL
                     LGIIFSPFLHTGFHHVASNSIPLLILLTVMLSFYQKIAFEALFYMVLFTDILVWILAR
                     PAYHVGASGLVYSLAAFLIAAGFFKRKAMSAFIAAAVALLYGGLVWGAVPGLVDWYIS
                     WEAHFFGATTGIYLAHFYKRKL"
     gene            complement(722..1726)
                     /locus_tag="PK683_08110"
     CDS             complement(722..1726)
                     /locus_tag="PK683_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH08452.1"
                     /translation="MENWFKNFFLYVMLILSLSELNAETFHLLSGEKIEGKIIKETRN
                     YIRVETSGGKTVKISKKNLSKNISSSPEISQNTEKDIETFLNGAKEGKTDLLKKKPQL
                     ADVKNNFGDTALILAADNGKLEAVKLLIQNKANIDEKNNEGETALMLACEGGYSEVVK
                     YLLQQHTDVNLRSNEGENALFLAVEKGYPDIVKLLIETNANVNAKNRNRETPLMLAAD
                     KGNLEIVRLLVNNDANVNSKNTDRETALMIAADRGNYEIVKSLIHNDANVNAKNKFGW
                     TALMMASYNGYLPIVKLLLHNEANVSIQNRNKQTALSMALQNRHLEIAELLKAAGAKE
                     "
     gene            2110..>2410
                     /locus_tag="PK683_08115"
     CDS             2110..>2410
                     /locus_tag="PK683_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HNH08453.1"
                     /translation="MNAFRYREDLLPASEFKGDYIYSPYRTGKKSWKKIALHLHSSEV
                     FFSQFRHTPEEIYRGYKSAGDYSGIVITDYMKITSVRNLQGWTLPGYEWGCNPKKK"
BASE COUNT          683 a          505 c          478 g          744 t
ORIGIN      
        1 actgcacata tccggaagga gtatctacaa taaatccccc tctgtgcaga tggataaggg
       61 ggtctacgga gatttcttca gcagagattt cttcaccatt tattatcatt ggacggttta
      121 aagcagatac aagattatta tcagtcctga gatactcaat agttttctga aattattcac
      181 aattttcttt tgtaaaaatg tgcaaggtag attccggttg tcgctccgaa gaaatgtgct
      241 tcccaggaaa tataccagtc cacaagaccg ggtactgctc cccagaccag tcccccgtaa
      301 agaagggcca ctgctgccgc aatgaaagcc gacatagctt ttctcttaaa gaatcctgct
      361 gcaatcagaa aggctgcaag tgagtaaacc agtccgctgg ctcccacatg gtatgcaggc
      421 cttgccagta tccagacaag tatgtctgta aaaagaacca tataaaacag cgcctcaaaa
      481 gcaatcttct gataaaagga aagcattact gtcaaaagta tcagaagagg gattgagttg
      541 gaggcgacgt ggtggaatcc cgtgtggaga aaaggagaga agataattcc cagaagtcca
      601 aaaaatgtcc taggataaat tccaagcctg gtaaagtcca gcggaatcag aaaatctgca
      661 attgtgatga tccataggag ggctacaagc cccagagaga ttttgatgct tttgtcgaat
      721 ttcattcttt agctcccgct gccttcagaa gttctgcaat ctccaaatgc ctgttctgca
      781 gagccataga aagagctgtc tgtttatttc tgttctgtat tgatacattt gcttcattgt
      841 gaaggagaag ctttacaatc ggaagatagc cgttatagga tgccatcatc agtgcagtcc
      901 agccgaactt attcttcgca ttcacatttg catcattatg aatcagggac tttactattt
      961 cgtaatttcc tctgtcagct gcaatcataa gtgcagtttc ccgatcagta ttctttgagt
     1021 tgacatttgc atcattgttt acaagaagtc tgacgatttc caggttgcct ttgtctgcgg
     1081 caagcattaa aggtgtttcc ctgttcctgt tcttggcatt cacatttgcg ttcgtctcta
     1141 taagaagttt tactatgtcc ggataaccct tttccacggc caggaacagg gcattctcac
     1201 cttcatttga acgaagattt acatcagtgt gctgctgaag cagatatttc accacttcag
     1261 aatacccgcc ttcacaggca agcatcagtg cagtttcccc ttcattgttc ttctcatcaa
     1321 tatttgcctt attctgaatc agaagtttga ccgcttcgag ttttccattg tctgcggcaa
     1381 gaattaatgc tgtgtctccg aaattgtttt tgacatcagc aagctgaggt ttctttttca
     1441 gcagatctgt ttttccttcc tttgcaccgt tcaggaaggt ttctatgtct ttttcagtat
     1501 tctggctgat ttcaggagag gatgagatat ttttcgacag attcttcttg gaaatcttga
     1561 cagtcttgcc tccggatgtt tccacccgaa tataattcct ggtttccttg attattttcc
     1621 cctcgatttt ttcccctgaa agaagatgaa atgtttctgc attcagttcg gaaagactga
     1681 gaatcagcat tacatataaa aagaaatttt tgaaccaatt ctccatagac atcattttct
     1741 atatttgacg taaaaattca tttgtagatc attcgggatc atttttattt gaaaacttct
     1801 ttttccttga aaaagtaaaa aaatcaggat aaatgcagtc cgaagatttt ctccttgaaa
     1861 ttaagtggtg caattttgca gtttaattct aaagtgacct ggtgccggag gaagaatctg
     1921 tctttggcgg acagactttg attttccaat ggaatcggaa gattcctttc cggacctgca
     1981 gtcttcagaa gacaggagct gaatcttttc aaatatgact ctgattaaaa ctgaaaaacc
     2041 atgaaaacaa gaatcgtctt tttactcttt cttacgctgc ccttttttta ttacctcatt
     2101 ctcaatactg tgaatgcatt cagatacagg gaggatcttc ttccggcatc tgaatttaaa
     2161 ggcgattata tttacagccc ttacagaacg ggaaaaaaat cctggaaaaa aattgcactt
     2221 catcttcaca gcagcgaggt tttcttttcc cagttcaggc atacccctga agaaatatac
     2281 agaggataca aatctgccgg cgactactca ggaattgtca ttacagatta tatgaaaatt
     2341 accagtgtca ggaatctgca gggatggact cttcctggtt atgaatgggg atgcaatccg
     2401 aagaaaaaac
//
LOCUS       DAOQGJ010000447         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28427_length_2407_cov_2.418793, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000447 DAOQGJ010000000
VERSION     DAOQGJ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28427_length_2407_cov_2.418793"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PK683_08120"
     CDS             complement(<1..695)
                     /locus_tag="PK683_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH08454.1"
                     /translation="MKVKFWGVRGSIGSPVSGAVIRKKIEKVLSLATPADIQNEDTIN
                     RFLSSLSFSTVGTYGGNTTCIEVRDSGDNLIIIDAGTGIRELGNSLLSTKFLQGKGKA
                     YWLFTHSHWDHIQGIPFFVPLYIPGNEFEIFSSMQDIESRIRLQHVSTHFPVPYEGLG
                     STRRFHFIPEGTQTQITPVLTALSKSVRHPGGSYSYKLKEGDKTFIFTSDAEFNIDEM
                     ENIESYIEYFSGA"
     gene            777..1082
                     /locus_tag="PK683_08125"
     CDS             777..1082
                     /locus_tag="PK683_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH08455.1"
                     /translation="MFKTVYDDSQKSCTILLDGVISLKSAIQLKDQIAREAEKGYIHV
                     VLDFDNSVHIDSSGIGALFNSQKFLNDKSGSLKIKNLSQDTLSILRIANLDKHLNII"
     gene            complement(1075..1512)
                     /locus_tag="PK683_08130"
     CDS             complement(1075..1512)
                     /locus_tag="PK683_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNH08456.1"
                     /translation="MIFPILLFAEEFTGKVVSVHDGDTVRVLLDGKVQVKIRLFGIDA
                     PETSQDFGKVSRDELRSLIHSKDVKVLSKGKDRYGRTLGIIFYENQDINLEMVKRGMV
                     WVYRRYSLDKDYLKAEEKAKSEKLGLWKQKNPVPPWEFRRESR"
     gene            complement(1517..2083)
                     /locus_tag="PK683_08135"
     CDS             complement(1517..2083)
                     /locus_tag="PK683_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08457.1"
                     /translation="MKKLKNCSFKSVYPQFSPKNSSYSAGARKINRMLKKEFLNISAH
                     FSESECEPEEKEGFYTLETDYFLKRNSRIVSILYISTGYKKDRPHPNNISKGFSFDAV
                     TGKRISFSDLIQGQKALNLLQDLIISEISAMDIPFSEEIRKKEYDFYITESGVSFINL
                     FQIHALQSVEAEIPFHKLEKILNVDKIR"
     gene            2182..>2407
                     /locus_tag="PK683_08140"
     CDS             2182..>2407
                     /locus_tag="PK683_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08458.1"
                     /translation="MKKILTSQIQTLDYGTVRQSGDTRKKMRGRIILFSVSLAVFFTG
                     CTSSVEFIPDGDYHFFKQPYLKTDAGEVDVL"
BASE COUNT          689 a          485 c          476 g          757 t
ORIGIN      
        1 tctgctcctg aaaaatactc tatatatgat tcaatgtttt ccatttcgtc aatgttaaat
       61 tcagcatctg aagtgaatat gaatgtttta tcaccttcct tcagtttgta ggaataactt
      121 ccgcctggat gacggactga tttggaaagt gcagtgagca cgggtgtaat ctgcgtctgt
      181 gttccttccg gaataaaatg aaatcttctg gttgatccta agccctcata cggaaccgga
      241 aaatgcgtgg acacatgctg cagtctgatt ctggattcta tgtcctgcat cgaactgaat
      301 atttcaaact catttccagg aatgtagaga ggaacaaaaa atggaattcc ctgaatatgg
      361 tcccaatggc tgtgagtaaa aagccagtat gcctttcctt tcccctgcag aaattttgtg
      421 ctgagaagcg aatttcccaa ttcccggata cccgttcccg catctatgat gattaaattg
      481 tcaccgctgt ccctgacttc aatgcaggtc gtgtttcctc cgtacgttcc tacagtggaa
      541 aaactcagtg aggaaagaaa acggtttatc gtatcttcat tctggatatc cgccggagta
      601 gccaacgaga gaaccttttc tattttttta cggatgaccg cgccggaaac gggtgaaccg
      661 atcgaacctc gtacacccca aaactttact ttcatgcggg aaatgatgaa aaaatctcct
      721 aaaaacgcaa gaaaaaatct tgaaaaaaaa aaggactgtc tgattattat ccagttatgt
      781 ttaaaacagt ttacgatgat tcgcaaaagt cctgcacgat ccttctggat ggagtcatca
      841 gtctgaaaag tgccattcag ctgaaggatc agatagccag agaagctgag aaaggttata
      901 tccatgtagt tcttgatttt gacaatagtg tccatataga ttcttccgga atcggtgctc
      961 ttttcaactc tcaaaaattt ttaaatgata aatcaggatc actgaagata aaaaatctga
     1021 gccaggatac tttgtccatt ttgagaattg ccaatttgga caaacatctg aatattatct
     1081 agattctctt ctaaattccc aaggagggac tgggtttttc tgtttccata gacccagttt
     1141 ttccgatttg gccttttctt ctgctttcag atagtcttta tccagactgt atcttctgta
     1201 gacccatacc atccctctct tgaccatttc gaggttgata tcctggtttt cataaaaaat
     1261 gatcccgagt gttcttccgt acctgtcttt tcctttggac agtactttta catccttgct
     1321 gtgaatcagg ctcctcagtt catctctgga aacttttccg aaatcctggc tggtttcagg
     1381 ggcatctatt ccgaacagtc ggattttcac ctgaactttt ccgtctaaaa gaactcttac
     1441 tgtgtccccg tcatgtacgg atactacctt tcccgtaaat tcttctgcaa aaagaaggat
     1501 tggaaagatc aaaaaactaa cgaattttat caacatttaa aattttctcc agtttatgaa
     1561 atgggatttc ggcttctact gactgtaaag catgaatttg aaaaagatta atgaaagaga
     1621 ctccggattc ggtaatatag aagtcatatt cctttttcct gatttcttcg gaaaaaggaa
     1681 tgtccattgc actgatttca gaaataatca gatcctgcag aaggttcagt gctttttggc
     1741 cctgaatcag atcggaaaaa ctgattcttt ttcctgtgac agcatcaaaa ctgaaccctt
     1801 ttgagatgtt gtttggatga ggtctgtctt ttttataacc ggtagagatg tacagaatag
     1861 atacaatcct gctgtttctt ttcagaaaat agtccgtttc cagtgtatag aaaccttcct
     1921 tctcttcagg ttcacactcg gactcgctga aatgtgcaga tatattcaaa aattccttct
     1981 tgagcattct gttgattttt cttgcaccgg cagaatagga cgaatttttt ggagaaaact
     2041 gaggatagac ggatttgaag gagcagtttt tgagtttttt cacggccgtt tttttggatt
     2101 ccagaagaga ttccgcataa actgttgtgc aaaacgaaaa aagccagaca gccagtatgc
     2161 agagtctgaa atttttacgg aatgaaaaag attttgacat cacagattca gactttagac
     2221 tatggaacag tgagacagtc cggcgatacc aggaaaaaaa tgcgtggaag gatcatttta
     2281 ttttcagttt ccttggcagt tttttttaca ggatgcacct cctcagtgga atttattccg
     2341 gacggggact atcatttttt caaacagcct tatctgaaga cagatgcagg cgaagtagat
     2401 gtactgt
//
LOCUS       DAOQGJ010000448         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28484_length_2404_cov_2.926777, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000448 DAOQGJ010000000
VERSION     DAOQGJ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28484_length_2404_cov_2.926777"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..582
                     /locus_tag="PK683_08145"
     CDS             244..582
                     /locus_tag="PK683_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08459.1"
                     /translation="MRTSSPAYFLCIVKLSNPKDSSMDHLPKSSLSRVAFIGNYLPRR
                     CGIATFTTDLCEAIADVYPKTTCIAIPVNDTETGYAYPPKVRFELEERDIESYHRSAE
                     FRSALSEQRH"
     gene            524..985
                     /locus_tag="PK683_08150"
     CDS             524..985
                     /locus_tag="PK683_08150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH08460.1"
                     /translation="MNHIIEVLSFGLLSPNKGIENVISALPRILKKHPNAVYIILGVA
                     HPHVVRNEGETYRLSLQWLAHEKHVESNVIFYNRFVRTEELIEFIGAADIYITPYLNS
                     AQRHTMRKRAYLYGRSMIWLCCQIIRILNTIQLWLYRFLSAINLDSKTDLS"
     gene            complement(922..2121)
                     /locus_tag="PK683_08155"
     CDS             complement(922..2121)
                     /locus_tag="PK683_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH08461.1"
                     /translation="MSSKTVLNFPKGSIIYMENTPNPGYIYILKSGEIAIQSYIKFSS
                     MNLHKYVAGDTFGFVSALTRNPHDSTLIASVDCVVIRLTIDSFIEYLRSNKDVFLKFI
                     TISSSKLRTFLEKLEPNSLELKASPEKLFKMSKEFEMMNERELSAFALKKYLTCEFST
                     PKNPEILKEAEDLLKKIRPGYEFPSYKEYIDGSGTVYPKGSIIFAEGEPNDYFYLVVK
                     GRIKISKVVDNKEMILGLLDRGEIFGEMAILNKQARAATAVAHEETVLRKYSADTLLE
                     TEDDILVKILQIIARRLWKGSQRVFIMQLNDLNLKLYAQLNILIADEIANDEQKSSQK
                     SFTFQISFEELKQMANIVFVDDSSDILKEIMSDKNIQRTQDAIRIQDKSVFESKFIAL
                     KKRYSQS"
BASE COUNT          704 a          476 c          424 g          800 t
ORIGIN      
        1 tattccctgg atgatattga gttttattat aaacctcttg agatatcagt tctgaagcgg
       61 gtgctgaaaa tcactgctct ccagagatta ttttatcgga ataaaatttt tttcctatgt
      121 ttcattacga ttctttttta ctctgacatt atcatttaaa attaaacttt taagttttca
      181 ttattccagt catcttcata ttctctctgt ttttctttca gacatcagcc tgacagagat
      241 attttgagga catcttcccc tgcatatttt ctctgcatcg taaaactttc aaacccaaag
      301 gattccagta tggatcatct cccaaaatct tctttgagca gggttgcttt catcggcaat
      361 tatctgcccc gcagatgcgg aattgccacc ttcacgactg atttgtgcga ggcaatagcc
      421 gatgtatatc ccaaaacgac atgcattgca attcctgtca atgatacaga gacagggtat
      481 gcttatcctc ccaaggtcag atttgaactc gaagaaagag atattgaatc atatcataga
      541 agtgctgagt ttcggtctgc tctctccgaa caaaggcatt gagaatgtca tctctgctct
      601 tcccaggatt ctgaaaaaac atcctaatgc agtgtatatt attctcggtg tcgctcaccc
      661 gcacgttgtc cgaaatgaag gagagactta caggctctca ctgcagtggc ttgctcatga
      721 aaaacatgta gaaagtaatg taattttcta taacagattt gtcagaactg aagagctgat
      781 agaatttatc ggtgcagcgg atatttacat cacgccgtat ctgaactctg ctcagagaca
      841 tacaatgaga aaacgagcct atctgtatgg acgatctatg atctggcttt gctgtcaaat
      901 catacgtatc ttaaatacga ttcagctttg gctgtatcgt tttttgagtg caataaattt
      961 ggattcaaaa actgatttgt cctgaatcct gatggcatcc tgagttcttt gaatgttttt
     1021 gtctgacata atttctttca aaatatcgga agaatcatca acaaaaacaa tattcgccat
     1081 ttgtttaagc tcttcaaatg aaatttggaa ggtaaaactt ttttgagaac tcttctgttc
     1141 atcatttgcg atctcatctg ctatcaggat atttaactgt gcatacagtt tcagatttaa
     1201 atcgttcaat tgcataataa atactctctg acttcctttc cataatcttc gagctataat
     1261 ttgcaggatt ttgactaaaa tatcatcttc tgtttccagc agagtatctg cagaatattt
     1321 tctaagtact gtttcctcat gagcgacagc tgtagcagct cttgcctgct tatttaaaat
     1381 tgccatctct ccgaatattt ctcccctgtc taacagaccc agaatcattt ctttattatc
     1441 aacgactttt gaaattttaa tcctgccctt gacaacgagg tagaaatagt cattcggctc
     1501 accttctgca aatataatgc ttcctttcgg ataaactgtg cctgaaccgt caatatattc
     1561 tttgtaagaa ggaaactcgt aacctggtcg gatttttttc agtaaatctt ctgcttcctt
     1621 gagtatttcc ggatttttgg gagttgaaaa ctcacaggta agatatttct tcagagcgaa
     1681 ggcagataat tcccgttcat tcatcatttc aaattcttta gacatcttga ataatttctc
     1741 cgggctggcc ttcagctcaa gtgaattcgg ttccagcttt tccagaaaag ttcgcaattt
     1801 gctggagctg atagtgatga attttaagaa tacatcttta ttggaacgca gatattctat
     1861 gaagctgtcg atagtcagac ggataactac acaatcgaca ctggcaatca atgtactgtc
     1921 atgaggattt ctagtcaaag cagaaacaaa tccaaaggta tcccccgcga catatttgtg
     1981 gagattcatt gaagaaaact taatatagga ttgaatcgct atttccccgg attttaagat
     2041 atagatatat ccgggattgg gcgtattctc catataaata atagaccctt tcggaaaatt
     2101 taaaacagtt ttagatgaca tgacaggaaa tacttaacgg ctattgtttt acttcttgat
     2161 tatttaaaca accaattttt agaattttaa gaacagtcat acaaaataat ttcaatgaat
     2221 gatctgatgc agctgaggct gccgatcaaa ttaatcgacc tgccgacttc ctagggtgag
     2281 cacccgggaa ctgactgaca cattccttgc aggactgcag cagatttcgt atgtattcag
     2341 tcttacagta taaggcacat atttacacta tataaaaaat ttatatggtg tgtgttaagt
     2401 tatg
//
LOCUS       DAOQGJ010000449         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28604_length_2398_cov_3.063167, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000449 DAOQGJ010000000
VERSION     DAOQGJ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28604_length_2398_cov_3.063167"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            837..>2398
                     /locus_tag="PK683_08160"
     CDS             837..>2398
                     /locus_tag="PK683_08160"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH08462.1"
                     /translation="MFRLTLILSSFLFFSCSEKNFDVNQGQIVLSDSSRIYDLKGEWR
                     FLPRNEPEASRIDYNDSEWRLFPVPTQWRTKGYADVNTAWYRLRIFLGSDFKGKNLGI
                     AVPVIFNSSRIYFNGKLISEKGIIDSSGNMLAAEDNSDIYKIDIDHLNFDGENILAIQ
                     AASFSALGGTVNSTFAGEYEICKRKYYRNQLWNSAVAAIFLFMGLYHLIIYYRVRREK
                     SYLYFSLTVLSMSLYTVGLFRLDFLGNTQMPVYYTFVSISLSLTGIFFLLAVYHFFDY
                     PITRLHTVLILVYSPIALFHMFSIFDDRLVYYRNIIVIYIASAADLLALCWAMGISVY
                     ALIQKKEQAFTLVLGLLLFVPMTFYDAIVKGYNVSYPTDWNFKAHSLVFNLMIAIAFS
                     AKYSKLYERLIQMQKDYSSELEREVERKTEELSHANSVKDKLFSVISHDLRSPLDMLD
                     SIINLVNTKTLSKKDLDRYIHQISEKLKYNRFLLENLLSWSATQLQREDMMLSLENVT
                     AVVAEVTMNMET"
BASE COUNT          690 a          443 c          508 g          757 t
ORIGIN      
        1 ttttatatct ctatgattca ccgccgaaaa ttatttggat tacttgcggg aatacttcca
       61 atgaatatct aaaaaaaatg ttatcagaaa aactgataaa agctgttgaa cttctgcagg
      121 gcggcgaaaa attagtagag attgtataaa tgtatataat agcattttta aaaaagatta
      181 aaaaatttcc ccctgccagc accaattcag tctccttttt aaaggcagcg ggacgcgaat
      241 tacactaatt ttcaagaatt attattttta attctcgtct tcttgaagcc cgctgctttc
      301 tgctttaaca gaccgtacga tgatcaggag tctgtaaaaa tttatttcta ttatccaaga
      361 gacagatttg attatacgaa atggagacag gatctgtatg aggggttatc agtccgagaa
      421 ctcagccgaa aggccctgtt ttttcgaaga cagatgaccg aggtgcgttc acgtcgatga
      481 gggcagattg cggagaaaaa aacagcctgt gcagaactgg gtgaatacct caaaggaaca
      541 gaataccgtt gaatttgttt ccccggagtg cttagatttg agccgacagt tttgacggca
      601 aaatcgatat cagtgtatgg aatctggcaa attcatccgc agtccggcag atgtgacttt
      661 tgggagacat tcattatcag tatgaaggga ggggatattg attcaattat tctctatata
      721 aaaagaagga tactatcaat attgacagta ttcctctttt ttaatccatg tttagcgaca
      781 ttttttaaaa attgtttact gcagatcgag ctttttttac tgcttgtttc tgtaatatgt
      841 tccgtttgac tctgattctg tcatcctttc tgtttttttc ctgttctgaa aagaactttg
      901 atgtgaatca gggacagata gtgctttccg attcatccag gatttacgat ctcaagggtg
      961 agtggagatt tctgcccaga aatgagccgg aggcttccag gattgattat aatgattctg
     1021 agtggcggct tttcccagta ccgacccaat ggaggaccaa aggatatgct gatgtaaata
     1081 cagcctggta ccgcctgagg atttttttag gaagtgactt taagggaaaa aatctgggga
     1141 ttgcagtgcc tgtaattttt aattcttcca gaatttattt taacggaaag ctgatttcag
     1201 aaaagggaat cattgacagt tcaggaaata tgcttgcagc tgaagataat tccgacatct
     1261 acaagattga catagaccat ctgaattttg acggagaaaa cattcttgcc attcaggcag
     1321 caagtttcag tgcactcggc ggaacggtaa attccacctt tgcgggggaa tatgagatat
     1381 gcaaaagaaa atactacagg aatcagcttt ggaactctgc agtggccgca atatttctgt
     1441 tcatgggact gtatcatctt attatatatt accgtgtgag aagagaaaaa tcatatctct
     1501 atttcagtct tacggtactg tccatgtctc tgtacactgt cggacttttt cgtctggatt
     1561 ttttaggaaa tacccagatg ccggtgtatt atacttttgt gagcatcagt ctgagcctga
     1621 caggcatttt ttttcttctg gcagtatatc atttttttga ttatccgatt accaggctgc
     1681 atactgtatt gatccttgtc tattctccga tcgcactctt tcatatgttt tccattttcg
     1741 acgacagact ggtctattac agaaatatca ttgtcatcta cattgcttcc gcagctgatc
     1801 ttctggcact ctgctgggcg atgggaattt ccgtctatgc tctgattcag aagaaagaac
     1861 aggcgtttac tcttgtactg ggactgcttc tttttgttcc gatgacgttt tatgatgcga
     1921 ttgtaaaagg gtataatgta agttatccta ctgactggaa cttcaaggcg cattcactcg
     1981 tatttaatct gatgattgca attgcgttct ctgccaaata ctccaagctg tacgaaagac
     2041 tgattcagat gcagaaggat tacagctccg agctggaaag ggaagtggaa aggaaaactg
     2101 aagaactcag tcatgcaaat tcggtcaaag ataaactttt ttcagtaatt tctcatgatc
     2161 ttagaagtcc tctcgatatg ctggattcca taatcaacct ggtaaataca aagactcttt
     2221 cgaagaagga tcttgacaga tatatccatc agatttctga aaagctgaag tacaaccggt
     2281 ttcttctgga aaatcttcta tcctggtcgg cgactcagct gcagagagag gatatgatgc
     2341 tttctctgga aaatgtcaca gccgttgttg ctgaagtgac aatgaatatg gaaacata
//
LOCUS       DAOQGJ010000450         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28606_length_2398_cov_2.880922, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000450 DAOQGJ010000000
VERSION     DAOQGJ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28606_length_2398_cov_2.880922"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1436
                     /locus_tag="PK683_08165"
     CDS             198..1436
                     /locus_tag="PK683_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08463.1"
                     /translation="MKKQVSLIFILLTVSTHIFSDTVTLRNGKVLENVRTKLSKDSVT
                     VTSADGKLITLAKTEVKSVRLRSVTMAAQNSTQAKAEAEKEKIRIADTLAETADWEID
                     PSLKPRVAVIQFKAGSGVSSAEAEIVTELVTVSFVKTKLFIIVDKKTMDDAAKNLSCN
                     SQDCSDMLMKKIKASKVITGSITKTGKRYFISGTINDPERDSVDFAENAVAESDAAFP
                     QASELLAKKAAGGIADYADIGVGAKGGDSKYEAAVKSLVLPGWGQYSYGTKSGSAWHR
                     GKGIAFGSLFVLGALNFVYRNQQLAENKKDYKSVHGTFLIMPAVTGAELLYYAKDRQA
                     FSDYKNSAQNLNQAVSIMGAVWILSLADSFFIGKRFLGFGDMEKKTGFNLNAAPSFVG
                     TANTSVKMEMNYELTYKIIF"
     gene            1498..2208
                     /locus_tag="PK683_08170"
     CDS             1498..2208
                     /locus_tag="PK683_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH08464.1"
                     /translation="MKMLFSYIGAVEPKVTSVLPKNGSTGNVRNTQIVMVMNTEMDAA
                     SLAAALSIKTDTDSVAGDIAVDGKIISYVPKAYLGSNTKHTITLGTGAKSQKNQSPSS
                     PITMTFSTSADVDLTPPSVVNTSPTDSSTGFPINAAILATFSETVNPSSFSSSMITLS
                     GGSIAGTAELNDSTISFKPSSNLPPNTVISARIAAGLKDMAGNKMTSQYTWIFTTGAK
                     EAKVCVLGTDKYGDCILQ"
BASE COUNT          758 a          507 c          589 g          544 t
ORIGIN      
        1 aaatgaagta ctgaagaagg aattggcgga gctgcgagag gaaatcagtt cactgaaaag
       61 gagcgctgta agggcacaca gcagtgtgcc ccaaccaatg ccccagccgt ctcagctcga
      121 aagtatagaa aagagactct ctgcaatgga gaggatggcg aaaaaatgaa aggtcagaat
      181 aaagcaaaga gggaaccatg aaaaaacaag tatcactgat atttatttta ctgactgtca
      241 gtacacatat attttcggat actgtcaccc tgagaaacgg caaggtgctc gaaaatgtca
      301 gaacaaagct gtccaaggac tcagtgactg tgacatccgc agacggaaag ctgatcacac
      361 tcgcaaaaac ggaagtgaaa tcagtccggc tgagatcagt cacaatggcg gcgcagaaca
      421 gcacacaggc caaggcggaa gcggaaaagg aaaagatccg tattgcggac actctggctg
      481 aaacggcaga ttgggaaatt gatccttcac tgaaaccgag agtggcagtc atacagttca
      541 aggcaggaag cggggtcagt tccgcagaag ctgaaattgt caccgaactg gtgacagtaa
      601 gtttcgtgaa gacaaagctt ttcatcattg tggataaaaa gacaatggat gatgctgcca
      661 aaaatctcag ctgcaattct caggactgct cggatatgct gatgaaaaaa atcaaagcat
      721 ccaaagtcat tactggctca attactaaaa ccggaaaacg ctactttatc agcggaacaa
      781 tcaatgatcc tgaaagggac tcagtggact ttgcggaaaa tgcagtggct gaatctgatg
      841 ccgccttccc gcaggcaagc gagctcctcg caaagaaagc agccggcgga atcgcagact
      901 atgctgatat cggagtcgga gccaaaggag gagacagcaa gtatgaggct gcagtcaaat
      961 ctcttgtgct cccaggatgg ggtcagtaca gctatggaac aaaatcagga agtgcctggc
     1021 acagaggcaa gggcattgct ttcggctctc tctttgtcct aggtgctctg aatttcgtgt
     1081 acagaaacca gcagcttgca gaaaataaaa aagattataa atccgtgcat ggcacatttc
     1141 ttataatgcc tgcagttaca ggtgcggaac ttctttatta tgcaaaggac agacaggctt
     1201 tcagtgatta taaaaattcg gcacagaatc tgaatcaggc agtgtcaatt atgggagctg
     1261 tctggattct gagtctggcg gattcctttt ttatcggaaa acgttttctc ggattcgggg
     1321 atatggaaaa gaagacaggc ttcaatctga atgcagcccc ttcttttgtc ggaacagcaa
     1381 atacatcagt gaaaatggag atgaattatg aactcactta taaaattatt ttttagtttc
     1441 tctgtgcttc tgctcggagc atgccggttt acaccttctg agggaattcc ttcaaatatg
     1501 aaaatgctct tcagctatat aggtgcagtc gagcctaagg tgacgtcggt actgccaaag
     1561 aacggaagca cgggcaacgt gaggaataca cagattgtga tggtgatgaa tacggagatg
     1621 gatgctgctt cgcttgcagc ggcattgagc atcaaaacgg atacggactc tgttgcaggc
     1681 gacatagcag tagacggaaa gatcatttcc tatgttccaa aagcatatct gggaagcaat
     1741 acaaaacata ccattacgct cggcacaggc gcaaagagcc agaaaaatca gtctccttct
     1801 tcacctatca cgatgacatt ctctaccagt gctgacgttg acctgacacc gccgtcggtc
     1861 gtaaatactt caccgacaga ttcaagcacc ggtttcccga tcaatgcagc cattctggca
     1921 acattttcgg aaaccgtcaa tccttccagt ttcagctctt ctatgattac tctttccgga
     1981 ggaagcatag caggaacagc agagctgaac gacagcacaa tcagtttcaa gccaagttcc
     2041 aatcttcctc cgaacacagt catatcagcg agaatcgcag caggactcaa ggatatggca
     2101 ggaaacaaaa tgacttctca gtatacctgg atatttacaa ccggagcaaa ggaagcaaag
     2161 gtctgtgttt taggaacgga taaatacggg gactgcatac tgcagtaatt acattataat
     2221 tattttataa caggagaaaa ttatgagaac agggaacaga aaaggatcgg gattatgaaa
     2281 aggactttat cattttttta tagggcgcgg gaaaggatga gttcagggct cagggatcag
     2341 cactcggaga ctgaatcgct gaattcagca aagcaatcca cctcagctac ggttggtg
//
LOCUS       DAOQGJ010000451         2396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28639_length_2396_cov_3.105938, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000451 DAOQGJ010000000
VERSION     DAOQGJ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28639_length_2396_cov_3.105938"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PK683_08175"
     CDS             <1..439
                     /locus_tag="PK683_08175"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08465.1"
                     /translation="TVFSLQCKKGINDLMKDSKVLESECESGKFRSCTQVGIRMAFGT
                     YGYRKDYSKAKTFLEKACSGEDGLGCKELAILHEYGRGTEKSLSKAAEYYKISCEKEE
                     AFGCFYLGKFYKKGMGVSKDPKEAEKYLRKACDLKLDMACKEL"
     gene            436..1284
                     /locus_tag="PK683_08180"
     CDS             436..1284
                     /locus_tag="PK683_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08466.1"
                     /translation="MKIRILTLNLHSWQENEQDRKLSLIADAVIKEEADFIAFQESAQ
                     NRNSPYADQYRFIRTDNTSKIISDRIREKSGKNYPYIWDWAHYGFDVYEEGIALLSCH
                     ECTGHESRYISESVSREDIFSRKVFGAQYLLNGIKINIFTVHVSWKASPLDGRAMTEL
                     RNLNEFVHHFENRFRGGFSFVCGDFNVNPVSGPLMSEGYFFMTENAKYSDTYLEIHRE
                     ANTHSHSEDHSSMIHAAPMDGRIDYIFRSEKNPFRTEKAEFIFTRKKYGLVSDHYGYI
                     AEFNLD"
     gene            1413..>2396
                     /locus_tag="PK683_08185"
     CDS             1413..>2396
                     /locus_tag="PK683_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH08467.1"
                     /translation="MKKSSDYIFQKLLFKGRNTDIYRGFEKNSEKKFIFKILSSDFPS
                     EQEIRRLKKEYELTKKIQSNYVIRTEGFERQGNRYFILLEDVEGETLKTVFGKREPDV
                     QHLLSVFSKISQGLYDIHSSGIIHKDINPQNIIYNNEKDIVKIIDFGISTDFIQESPN
                     FRNPNRVEGTISYIAPEQTGRVNRNIDFRIDFYSLGVTFYELLTGSLPFSSDEPMEMF
                     YAHIAKSPVSPEKMNSRIPSAVAEIVLKLLEKNPDDRYNTALGLKKDLDFCLRELKQN
                     GTVLGFTAGKKDYSSTFKIRGRLYGRENELKVLEDAYRNSVSGRPELVLVSG"
BASE COUNT          795 a          449 c          512 g          640 t
ORIGIN      
        1 aacagtattc tctctgcagt gcaaaaaggg aataaatgat ctgatgaaag actcaaaggt
       61 gcttgaatca gaatgcgaat ccggaaaatt cagatcctgc actcaggtcg ggatcagaat
      121 ggcatttggc acttacgggt accggaaaga ctattcaaaa gcaaagacat ttcttgagaa
      181 ggcttgttcc ggtgaagatg gactcggatg caaggaactg gcgattctgc atgaatacgg
      241 cagaggaaca gaaaagagtt tatcaaaggc ggcagaatat tataagatat cctgcgaaaa
      301 agaggaagct ttcggatgct tttacctcgg caagttttat aaaaaaggaa tgggagtttc
      361 caaagatccg aaagaggccg agaaatatct aaggaaagcc tgcgatctga aactcgatat
      421 ggcctgcaag gaattatgaa aataaggata ctcactctga atcttcactc ctggcaggaa
      481 aatgagcagg acagaaaact gtctttaatt gcggatgccg tcattaaaga agaagcagat
      541 tttattgcat ttcaggaatc tgctcaaaac agaaacagcc cctatgccga tcagtacaga
      601 ttcatcagaa cagacaatac atcaaagatc atttcggaca ggatcagaga aaagtcagga
      661 aaaaactatc cttacatctg ggactgggca cattatggtt ttgatgtcta cgaagaagga
      721 atagcacttc tttcctgcca tgaatgcacc ggccatgaaa gcagatatat ttccgaatca
      781 gtgagcagag aggatatttt ttccagaaaa gtctttggag cacagtatct tctgaacgga
      841 ataaaaataa atatatttac tgtccatgtt tcctggaaag catctcctct ggacggacgt
      901 gcaatgacag aactcagaaa tctgaatgag tttgtgcatc attttgaaaa ccgtttcagg
      961 ggaggctttt cttttgtctg cggcgatttc aatgtaaatc ctgtttcagg ccccttgatg
     1021 agtgaaggat attttttcat gacagaaaat gcaaaatatt cagatacata tctggaaatt
     1081 catcgggaag caaacacaca ttctcattct gaggatcatt cttcaatgat tcatgccgct
     1141 ccaatggatg ggaggattga ttacatattc cgatcagaaa aaaatccttt cagaactgaa
     1201 aaagctgaat tcatatttac aaggaaaaaa tacggactcg tttccgatca ttatgggtac
     1261 attgcagagt ttaatctgga ctgaattcgc atatcagagg tgcccggcag gggttaacac
     1321 ctactgtttc cagttttgag aaactttctg ttttttattt tgactctcag ggagcagcaa
     1381 ttttaccttg aattcacggt ttcaaacgac agatgaaaaa atcttctgat tatatttttc
     1441 aaaaactgct tttcaaagga agaaacacgg atatttacag gggctttgaa aaaaactccg
     1501 aaaaaaaatt catttttaaa atattaagtt cagattttcc ctctgagcag gaaatccgaa
     1561 gactgaaaaa ggaatacgaa ctcacaaaaa aaatacaatc taattatgta attcggaccg
     1621 aaggctttga aaggcagggt aacagatatt ttattcttct ggaagatgtc gaaggtgaga
     1681 ccttaaagac tgtttttgga aagagagaac cggatgtaca gcatctcctc tctgtttttt
     1741 ccaaaatttc gcagggactg tacgacatac acagctccgg catcatacat aaggatataa
     1801 atcctcagaa tataatttat aataatgaaa aagacattgt caagataatt gatttcggaa
     1861 tttcgaccga ttttattcag gaaagcccca atttccgaaa tcctaacaga gtggaaggca
     1921 caatatctta tattgcaccg gagcagaccg ggagagtaaa caggaatatt gacttcagaa
     1981 ttgatttcta ttcgcttgga gtcacttttt atgaactgct tacaggcagt cttccgttca
     2041 gttcagatga gccgatggaa atgttctatg ctcatattgc aaaatcacct gtatctcctg
     2101 aaaaaatgaa ttccagaatt ccttctgccg tcgcagagat cgtactcaag cttctggaaa
     2161 aaaatccgga tgacaggtac aacacagctc tcgggctgaa gaaagatctc gacttctgtc
     2221 tgagagaact gaagcagaac ggtacagtcc tggggttcac tgccggcaaa aaggactatt
     2281 ccagtacatt caagattcgc ggaagacttt acggaagaga gaatgaactc aaggttcttg
     2341 aagatgctta cagaaactcc gtttcgggca gacctgaact ggttctggtc tcaggt
//
LOCUS       DAOQGJ010000452         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28649_length_2395_cov_4.261966, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000452 DAOQGJ010000000
VERSION     DAOQGJ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28649_length_2395_cov_4.261966"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..2185
                     /gene="pta"
                     /locus_tag="PK683_08190"
     CDS             110..2185
                     /gene="pta"
                     /locus_tag="PK683_08190"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNH08468.1"
                     /translation="MSKSLYITSAEPKSGKSLISLGIMELLKSTVSKVAFFRPSVSNT
                     ESDPDIELIMKNYNLQMDYKDTYGISLEEALKLMSEDRKADLIDIILSKFRKLEENFD
                     FILIEGSDFKSASRAFELEINSEIAKNLGAPVIAVCPATDKDEKETAAAAKLAVESYS
                     SSGCTVIGLFITRSEKEKIPALKKLVSADTDPSILTYYIPENDILRTLTMNEIRSELD
                     AEILFGHEYLFRHAHHTLVAAMRLENFLSHLKEGTLVIVPGDRADIILGGFVCSENKN
                     FPRIMGIILTGGIYPDPSIRKIMDGIDAHIPVLLTKEDTLRTVDRISRIDSKISPNDK
                     IKIAACHALFESSTERIRLKEKIIIHRSTVVTPKMFEYDLIQKAKSSHRHIVLPEGSE
                     ERILRAAEVLLHREVAKITLLGNEAQIRDKIKKLGLKVGGAEIIDPAASPLFKEYCDT
                     YFELRKHKGITPDNARDVMSDASYFGTMMVYKGHADGMVSGSVNTTQHTIRPALEFVK
                     TKPGCSLVSSVFLMCLPDRVLVYGDCAVNPNPSAKELAEIAISSAETASKFGIEPRVA
                     MLSYSTGESGKGEDVEKVREAVKIAREKYPSLLIEGPIQYDAAVDPDVAKTKMPDSPV
                     AGQATVFIFPDLNTGNNTYKAVQRSANAVAVGPVLQGLNKPVNDLSRGCTIPDIINTV
                     AITAIQAGD"
     gene            2190..2309
                     /locus_tag="PK683_08195"
     CDS             2190..2309
                     /locus_tag="PK683_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08469.1"
                     /translation="MKNILVINTGSSSIKYQLFDMTEKTVLASSWKVRVLSTL"
BASE COUNT          729 a          534 c          542 g          590 t
ORIGIN      
        1 tttgaatcca agcagtttta tttcccgaat ttccgggaga atgccgcaat ttttccggct
       61 gtctgcgata agacttgttc agaaacgaag gttctgtaaa taaggaatta tgtcaaagag
      121 cctatacatc acttcagcag aaccgaaaag cgggaaatca ctgatttctc tcgggattat
      181 ggaactgtta aaatccacag tttccaaggt ggcatttttc agaccctcag tgtccaacac
      241 tgaatcggat cctgacattg aacttatcat gaaaaattat aatcttcaga tggattataa
      301 agatacatat ggaataagtc tggaagaggc actgaagctg atgtccgaag acagaaaggc
      361 cgatctgatc gacatcatac tttccaaatt cagaaaactg gaagagaatt ttgactttat
      421 actgattgag ggatctgact tcaagagtgc ctcgagagca tttgaactgg aaatcaattc
      481 cgaaattgca aaaaacctgg gggctcccgt aattgcagtc tgccctgcga cagacaagga
      541 cgaaaaggaa actgcggcag cggcaaaact tgcagtggaa tcctattcct catccggatg
      601 cactgtgatc ggccttttta ttacaagatc cgaaaaggaa aaaatccctg cacttaaaaa
      661 actggtctcg gcagacacgg atccttccat tctcacttat tatattcctg aaaacgatat
      721 actaagaaca cttacaatga atgaaatccg gagcgaactg gatgcagaaa ttcttttcgg
      781 gcatgagtac cttttcagac atgctcatca tactcttgtt gctgcgatga ggctcgaaaa
      841 cttcctcagc catctgaagg agggaacact tgtaatcgtt ccgggagaca gggcagatat
      901 catcctcggc ggttttgtat gttctgaaaa caaaaatttt ccgcgaatca tgggaatcat
      961 acttacagga ggcatttatc ccgatccgag catccgcaag atcatggacg gtatcgacgc
     1021 tcatattcct gttcttctca cgaaggaaga cactctcaga actgtggaca gaatcagcag
     1081 aatcgattcc aaaatttctc ccaacgacaa gatcaagatt gccgcctgtc atgctctttt
     1141 cgagtccagc acagaaagaa tacgtcttaa ggaaaaaatc attattcaca gatccacagt
     1201 cgtcactcct aaaatgttcg aatacgacct gatccagaag gcaaaatcct ctcacagaca
     1261 tattgttctt ccggaaggtt cagaggaaag gattctccgg gcagccgagg ttctccttca
     1321 cagggaagtg gcaaagatta ccctcctggg caatgaggct cagattaggg ataaaatcaa
     1381 gaagctggga ctgaaagtgg gcggtgctga aatcattgat cctgcagcct cccctctttt
     1441 caaggaatac tgtgatacgt attttgaact gagaaaacac aagggcatca cgccggacaa
     1501 tgcgagagac gtgatgagtg atgcaagtta tttcggaaca atgatggttt ataaaggaca
     1561 cgctgacgga atggtctcag gttcagtcaa cacaacacag catacaatcc ggcctgctct
     1621 cgaattcgta aagacaaaac cgggatgttc tcttgtatcc agtgtatttc tgatgtgcct
     1681 tccggacaga gttctcgtct acggagactg tgcagtgaat ccgaacccga gcgcaaagga
     1741 actggcagag attgcaattt cttctgcaga gactgcatcc aagttcggca tcgaaccgag
     1801 agtggcaatg ctctcctact ccactggaga atccggcaaa ggagaggatg tggaaaaagt
     1861 ccgtgaagca gtgaaaattg caagagagaa atatccaagt cttcttatcg aaggcccaat
     1921 ccagtacgac gccgcagtag atcccgacgt tgcaaagaca aagatgccgg acagccctgt
     1981 tgcaggacag gcaaccgtat tcatctttcc tgatctgaac acaggaaaca atacatacaa
     2041 ggcagtgcag agatctgcaa atgcagttgc agttggacct gtgcttcagg gactgaacaa
     2101 gcctgtaaac gatctgagca ggggatgtac aattcctgat atcatcaata cagtcgcaat
     2161 tacagcaata caggcagggg actgagataa tgaaaaatat acttgtaatc aatacaggaa
     2221 gttcttcaat caagtaccag ctttttgata tgactgaaaa aacagttctg gcctcttcct
     2281 ggaaggtgag ggtgctgtca accctctgaa ttcctgttga gcctaaattg tcttgctggc
     2341 tcactgattg ataggttatg ccccgtctga gacgtgatct acgatttccc tcatg
//
LOCUS       DAOQGJ010000453         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28700_length_2393_cov_3.096664, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000453 DAOQGJ010000000
VERSION     DAOQGJ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28700_length_2393_cov_3.096664"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="PK683_08200"
     CDS             complement(<1..944)
                     /locus_tag="PK683_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNH08470.1"
                     /translation="MSLLKGRTRKILCGCGLAYFSIFAGACTIFKKDTAEFREKIPSA
                     VRIKLAKFTDEIEVFSDRSIHAESAGFDRVFENRFSLKEHYKENLSSELKLQFSDEKF
                     RFKDRTYRGSLKIVPILSGGYYAVNVLPIESYLLSVVPSEMPAGWHEEALKTQSVAAR
                     TYAVYQMLQNRDREFDMEADVSSQMYLGIRQENSRTTNAVNATKNQVLIHSGKPIVAY
                     YHSHSGGKTASAEEVWGQKLEYAVPRESEYCDLGNKMEWSLSENWDSVSKKLGPLNLG
                     EIRDIRKITDTPSGRADTIEVVGSNSKTEIKGQDFRRM"
     gene            complement(1008..2309)
                     /locus_tag="PK683_08205"
     CDS             complement(1008..2309)
                     /locus_tag="PK683_08205"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNH08471.1"
                     /translation="MKIIKARASSVLPDLNSAVPFEGRTYSIPYLNIGDEVEFEPKRG
                     WNRVISVESAEPDISRCRYFRKCGGCKAQHLEYAEQFRIKTAPHTEYYIRNFDVSPIL
                     VPAEVQYRYRNRMDFAVFPEKIGLRQEGNFRNIIDVSSCELQSEEADRILSECRNIVP
                     AGLAYDRKKETGFLKYITLRTDSDRKETMTVFTFTEDFRDTYEEKLFREKAASISSDS
                     VIFCFNRKKSEVSADGEYSVIKGRSLFTENILGKNADIPFNSFFQPNLKGFYPILSFI
                     EKELLNIEDRKMMADFFCGTGLFSIVFAEYFETAVGFDISADSVRLAEQNVIKIYPEK
                     KILFRQMDLFSIREKINSFADISGSAVFLDPPRAGIGTSMADLLLDLKPKHIFYISCN
                     PYSQETDLEILKTSYRLKSILITDPYPNTPHLESVAFLERL"
BASE COUNT          637 a          556 c          455 g          745 t
ORIGIN      
        1 atcattcttc tgaaatcctg acccttgatt tctgtctttg aatttgatcc gacaacttca
       61 attgtatccg ctctgccgga aggagtatct gtaatttttc gaatatcccg tatttctccc
      121 agattcaatg ggccaagttt tttgctcaca gaatcccagt tttccgaaag ggaccattcc
      181 atcttatttc ccagatcaca gtattccgat tctctgggaa cggcatactc aagtttctgc
      241 ccccagactt cttcagcaga tgcagttttt cctccgctgt gactgtgata atatgcgaca
      301 atgggctttc ccgaatgaat gagtacctga tttttcgttg cattgacagc atttgttgtt
      361 ctggaatttt cctgtctgat tcccagatac atctgggagc ttacatctgc ctccatatca
      421 aactctctgt ctctgttctg aagcatctga tacactgcat aagtccttgc agccacagac
      481 tgagtcttca gcgcctcctc gtgccagcct gcaggcattt cagaagggac tacggacaga
      541 agataacttt caatagggag aacatttact gcataatatc cgccgctcag tataggtaca
      601 attttcagag aaccgcggta tgttctgtcc ttgaatctga atttctcatc tgaaaactgc
      661 agcttcagtt ctgaagagag attctccttg taatgctctt ttaatgaaaa tcgattctca
      721 aataccctgt caaatcctgc actttctgca tgaatgcttc ggtcgctgaa tacttcaatt
      781 tcatcagtaa actttgccag tttaattctg actgcagaag gaattttttc ccgaaactca
      841 gcggtatcct ttttgaagat tgtacatgct cctgcaaaaa tggaaaaata tgcaagtccg
      901 catccgcata aaatctttct ggttctgcct ttcaataaac tcatcactca tattctcctt
      961 ataagtctta ttctgccgga cgggctaagc atcacagctg ctttgtatta cagtctttcg
     1021 aggaaagcga cagattccag atgcggtgta ttcggataag gatctgtaat caggatgctc
     1081 ttcagacggt atgaagtctt tagaatttcc aggtcagttt cctgagaata ggggttgcag
     1141 gatatataaa aaatatgctt cggcttcaga tccagcagaa gatcagccat tgaagttccg
     1201 attcctgctc tgggaggatc cagaaaaact gctgaccctg aaatatctgc aaaagaatta
     1261 attttctccc gtatggaaaa cagatccatc tgcctgaaaa gaatcttttt ctccggatat
     1321 atcttaatta cattctgttc tgccagtctg acagagtcgg cagaaatgtc gaatccgact
     1381 gcagtttcaa aatactctgc aaagacgatg ctgaaaagcc ctgtcccgca gaaaaaatca
     1441 gccatcattt ttctgtcctc aatattcaag agttccttct caatgaaaga cagaatcgga
     1501 taaaaaccct tcagattcgg ctgaaaaaag gaattgaaag gaatatccgc atttttgccg
     1561 agaatatttt ccgtaaaaag acttcgcccc ttaatgacag agtattctcc gtcggcagaa
     1621 acttcggact ttttacggtt aaagcagaat attacactgt ctgaactgat actggctgct
     1681 ttttccctga ataatttctc ctcatatgta tctctgaagt cctcagtgaa cgtaaagact
     1741 gtcattgttt cctttctgtc ggaatctgtt ctgagagtaa tgtatttcag aaagcctgtc
     1801 tctttttttc tgtcgtatgc aagtcctgcc ggaacaatat ttctgcattc agaaagtatt
     1861 ctgtctgctt cttccgactg cagctcacag gaacttacgt caatgatatt tctgaaattt
     1921 ccttcctgtc tcaggcctat cttttcaggg aagacagcga aatccattct gtttctgtac
     1981 ctgtactgaa cttcagccgg gacaagaatc ggactgacgt caaaattccg gatataatat
     2041 tcagtatgag gagctgtctt gattctgaac tgttccgcat actccagatg ctgagccttg
     2101 cacccgccgc attttctgaa gtacctgcat ctggaaatat ccggctctgc tgattccaca
     2161 gagataaccc tgttccagcc tcttttcggc tcaaattcca cctcatctcc gatattcaga
     2221 taaggaatgg aatacgttct tccctcaaaa ggaacggcag aattcagatc aggcagaaca
     2281 gatgaggccc tggcttttat gatcttcatt ggttcagaat ttctaatttt acttcactca
     2341 gagattccag acttccggtt ttcagaagtg cctgaagagt gtgtttttca gaa
//
LOCUS       DAOQGJ010000454         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28740_length_2391_cov_2.850171, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000454 DAOQGJ010000000
VERSION     DAOQGJ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28740_length_2391_cov_2.850171"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2322
                     /locus_tag="PK683_08210"
     CDS             <1..2322
                     /locus_tag="PK683_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HNH08472.1"
                     /translation="VARQAGAKDEAKKIARKLNVSVTPGIDNITALTLISKCKDKAGL
                     EAAAKSNKLDFKYNDAVSLEDNAETLLQLSYAAVVDLVTIPELQDEALVRSEEIWKKY
                     PSNRIRFKYIGGGGGKGQRVIKEKSQVKDAVMEILAESKVTSVGSNRNFLIELNIENT
                     RHNEIQLVGNGEWSLSLGGRDCSLQMHEQKLLEISNTVELLEEEIKNAENPQKAAVLK
                     GDLQVLKEMEEQSERFGAAVKLNSVSTFESIVEGTNHFFMEMNTRIQVEHRVTEMAYR
                     LKFTNPADKNDYFILESLIECMALLSLHGKRVPKPERILRNISGGEIRVNATNQSLQP
                     HAGGVIQTWSPPIEHEIRDDQGIGVKNPDTNIFIHYKLAGAYDSNVALIVSYGKSRRE
                     NLERLSEIIRVTELRGNDLQTNMPVHYGLINWILGKDPMFKPNTRYMMSYLAGIGSLF
                     SYSKDVDAEIVWAELSKQILAKNPERAKVLARKMTLILRPIKEVCANPHILAGFIGFN
                     SSHSWKEENGKVKFLINPVRILNDLYRYLNLEKSEKKAPCDQIWDHDAEILEAAISFY
                     DEIDSLSGKKLNLADLDALFAKDKNPFAGKISDESWAKCRESHTSFQLGMSALSIIPG
                     LGIKSGFSKLGVDANLDPIVPDEFANPDSMNKLVKLLNPPPKAKSDEIVAPMGGMFYS
                     REAPNLPVLISEGDQFKAGQPLFIIEVMKMFNKIAAPFSGTVVKNMMNDSDGKIVSKG
                     QTIFKIQPDEILQEETPEEILARRKALAAALLN"
BASE COUNT          768 a          478 c          574 g          571 t
ORIGIN      
        1 gttgcaagac aggccggtgc aaaagacgaa gcaaagaaaa ttgcaagaaa gctgaatgtg
       61 tctgtgactc cggggataga caatatcact gcactgactt taatcagcaa atgcaaggac
      121 aaggcaggac tggaggcagc agcaaagtca aataagcttg attttaaata taatgatgct
      181 gtatccctcg aggacaatgc agagacactg cttcagcttt cttatgcagc agtcgtagat
      241 ttggtgacaa ttcctgaact tcaggatgag gctcttgtca gatccgaaga aatctggaaa
      301 aaatatcctt cgaacagaat ccgtttcaaa tacatcgggg gcgggggcgg aaaaggccag
      361 agggtcatta aggaaaagtc tcaggtaaaa gatgcagtga tggaaattct ggcagaatcc
      421 aaagtgacaa gcgtcggatc aaacagaaac ttcctgatag aactgaatat tgagaataca
      481 agacacaacg agattcagct ggtaggaaac ggagaatggt ctctctcact gggaggaaga
      541 gactgttcac ttcagatgca cgagcagaag ctccttgaga tttccaatac agtggaactt
      601 ctggaagaag aaatcaagaa tgcagagaat cctcaaaagg cagcagttct caaaggagac
      661 cttcaggtac tgaaggaaat ggaagagcag tcagaaagat tcggtgcagc tgtaaaactc
      721 aacagcgttt ctacatttga atccatcgtg gaaggaacta accacttctt catggaaatg
      781 aatacaagga ttcaggtgga gcacagagtg actgagatgg catacaggct caagtttacg
      841 aatcctgctg ataaaaatga ctatttcata ctggaaagcc tgattgagtg tatggctctt
      901 ctttctcttc atggaaaaag agttccgaag cccgaaagaa ttctcagaaa tatttccggc
      961 ggtgagattc gggtcaatgc aaccaaccag agtcttcagc ctcatgcggg cggtgtgatc
     1021 cagacttggt ctcctcctat tgaacatgag atcagagacg atcagggtat cggagtaaaa
     1081 aatcctgata ctaacatatt tattcattat aaactggcgg gggcatacga ttcaaacgta
     1141 gcactgatcg tttcctacgg aaaaagcaga agggaaaatc tggaaagact ctctgaaatt
     1201 atccgcgtga ccgaactcag aggaaatgat cttcagacaa atatgccagt gcattacgga
     1261 ctgatcaact ggatactcgg aaaagatcct atgttcaaac cgaatacaag atatatgatg
     1321 tcgtatcttg cgggtatcgg atcactgttc tcctattcaa aggatgtaga tgctgagatc
     1381 gtctgggcag aactgtccaa gcagattctt gctaaaaatc ctgagagggc aaaggtgctt
     1441 gcacgcaaga tgactctgat cctgagaccg atcaaggaag tatgcgcaaa tcctcatata
     1501 ctggcaggct ttatcggatt caacagcagt cattcctgga aggaagaaaa cggaaaagta
     1561 aaattcctta ttaatccggt cagaatactg aatgatctgt accggtatct gaatctggaa
     1621 aagtctgaaa agaaagctcc atgtgatcag atctgggatc atgatgctga aatcctggaa
     1681 gcggcgattt cattctatga tgaaattgat tcactctccg gaaagaaact gaatctggca
     1741 gatttggatg ctctttttgc aaaagacaag aatccgtttg caggaaaaat ttcagacgag
     1801 tcctgggcaa aatgcagaga atctcacaca tccttccaat tagggatgtc tgctttgagc
     1861 attattcctg gtctgggaat caagtccgga ttctcaaaat tgggagtgga tgcaaatctc
     1921 gatccgattg ttccggatga atttgcaaat cctgattcaa tgaataagct ggtcaaactg
     1981 ctgaaccctc ccccaaaggc aaaatctgac gagatcgttg ctccaatggg cgggatgttc
     2041 tattccagag aggctccgaa tcttccggtt cttatatccg aaggggatca gttcaaggca
     2101 ggccagcctc tcttcatcat tgaagtaatg aagatgttca acaagattgc tgctccgttc
     2161 agcggaacag tggtgaagaa tatgatgaac gattcagacg gaaagatcgt atcgaaaggg
     2221 cagacaattt tcaaaattca gcctgacgaa attctgcaag aagaaactcc agaggaaatt
     2281 ctcgccagaa gaaaagctct tgcagccgct cttctgaatt aaaaaggttg aggttgtgta
     2341 aaagcatacc tcgatacaat tttcaccgcc ggtcaccgag cgatgtgccg a
//
LOCUS       DAOQGJ010000455         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_28995_length_2378_cov_2.957813, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000455 DAOQGJ010000000
VERSION     DAOQGJ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28995_length_2378_cov_2.957813"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1976)
                     /locus_tag="PK683_08215"
     CDS             complement(<1..1976)
                     /locus_tag="PK683_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH08473.1"
                     /translation="MNTDEIPGSLAERWKDMLDFAAELAEVSAVLFIRTGNENLRILV
                     SSNTEKSLINQEVMQRNWDEEIVKLGMTDCLEIPIHSADGTFLGKLCVGSGRGNTVPE
                     VYANLLCSFRDIIEDDLKGTDAGKKTLGSVHIRSRTAELERQLQFFKRIADHTYDWEV
                     FRNENGQIVYINQAFERITGFKTDDILNGKITEKDVVHPDDFFYVSESILRSVQKLQT
                     VDDLQFRVLRKDGRIRYVNLCASPVFENEIFIGTRTSVRDITEQHSFLELQAVKEELE
                     FHRVQLEKKNQELEKREKYFRTLIENAPDGVVLVGPDGKMKYASPAARRMFGFVEEKG
                     ILPDPAENTHPDDLARVLSELQKVIENENYSPKVEYRFLTADQGFRWIESTFTNQYQN
                     EDIKALIINFRIIDDKKKVELSLHRSEEFLKESERIANMASWEYDILQDRLYWSDNCH
                     LFYGYRLGEIDPSYELFKSHVHPDDLHIVSEKFESIFSDGKPYTGEMRILLQSGVQKW
                     VQNNVEAVWENGRIVKLKGIQLDISERKEAIEKLRMSEERYLQFITQAFEAISRTEFD
                     RPVDISLPAEQQIDLIYENAYLAECNQAMADMYNTTVEKFIGTRLINAHGGKDNTTNR
                     DAFRRFIQSGYRSTNDETEELTVDGRKVWFLSNTV"
BASE COUNT          627 a          582 c          416 g          753 t
ORIGIN      
        1 acagtattgc ttaaaaacca tactttgcgc ccgtcaactg taagctcttc cgtctcatca
       61 ttcgttgaac gataaccgga ttgaatgaat cgtcgaaatg catcacggtt ggttgtgttg
      121 tcttttccgc catgggcatt aatcagtcgg gttccgatga atttttcaac ggtagtgtta
      181 tacatatctg ccattgcctg attgcattct gccaaatatg cattctcata aatcaagtcg
      241 atctgctgct ctgcagggag cgaaatatcc acaggtctgt caaattcagt acgagatatg
      301 gcctcaaaag cctgagtgat gaactgcaga tacctttcct cactcattct taatttttcg
      361 atcgcttcct ttcgttcaga aatatcgagc tggattcctt tcagtttgac aatgcgcccg
      421 ttttcccaga ctgcttccac attgttctga acccatttct gcactccgct ctgcagaaga
      481 attctcattt cgccggtata gggttttcca tcagagaaaa tggactcaaa cttctcgctt
      541 acaatatgca gatcatccgg atgaacatga cttttgaaaa gctcatagct gggatctatc
      601 tcacctagtc tgtacccgta aaaaagatga cagttatctg accagtaaag cctgtcctga
      661 agaatatcat actcccagct cgccatattc gcaatccttt ctgattcttt caggaattcc
      721 tcactccgat gcaggctcag ctcaactttc tttttatcat ctattatcct aaaatttata
      781 ataagtgctt ttatatcttc attctggtac tgattcgtaa aagtgctctc tatccagcga
      841 aaaccttggt ctgccgtaag gaacctgtat tcaactttag gactgtaatt ctcattttct
      901 ataaccttct gcagttccga gagaactctt gcaagatcat ctgggtgcgt attttctgca
      961 ggatcaggga gtatcccctt ttcctcaacg aatccgaaca ttcttctggc tgcgggactt
     1021 gcatacttca ttttcccgtc gggaccgact aaaaccactc cgtcaggggc attttcaatc
     1081 aaggttctga aatacttttc ccttttttcc aattcctgat ttttcttctc cagctgaact
     1141 ctgtgaaatt caagttcttc cttgacagcc tgcagttcta aaaaactgtg ctgctcagtg
     1201 atgtctctga ccgaggttct ggttccgatg aaaatctcat tttcgaaaac aggagaagca
     1261 cacagattca catatcttat tcttccgtct tttctgagca cccgaaactg cagatcatca
     1321 actgtctgaa gcttttgaac agaccttaga atactttcgc ttacataaaa gaaatcatca
     1381 ggatgaacga catccttttc agtgattttt ccgttcagaa tatcgtctgt tttgaaccct
     1441 gtgattctct caaatgcctg gttgatataa acgatttgac cgttttcatt ccggaacact
     1501 tcccaatcgt atgtgtgatc agcaattctt ttgaagaact gtaattgtct ttccagttca
     1561 gctgtccttg atctgatatg cacactgccc aatgtttttt ttccagcatc agtgcccttt
     1621 aaatcatctt caataatatc tcgaaaactg cataatagat ttgcatatac ttctggaaca
     1681 gtatttcctc tgccggaacc aacgcatagt ttacccaaaa aagtcccatc cgcagaatga
     1741 atcggaattt caaggcagtc agtcattcct aatttgacaa tttcctcatc ccagttcctt
     1801 tgcataacct cttgattaat cagacttttt tcagtattgc tggataccag gattcgaaga
     1861 ttctcatttc ccgttcttat aaacaatacg gctgatactt ctgcaagttc agctgcaaaa
     1921 tcaagcatat ccttccatct ctctgccaaa ctgcctggaa tttcatctgt attcatatta
     1981 tatttccaaa accaatctca aagcttttca aaacatagga aggaaagaaa gcaggaaaag
     2041 caagagtttt tcagctctca agattctgag gttaagactc tcatcctcac tgcaggaagc
     2101 tttgactgca ttaatataac ttatcacggc tgacccacac tgctgaataa tcagcaacag
     2161 actgtcatat atgcggaata atgcctcgac ttgtgctgag cgatgtatca ggtatgcagg
     2221 tcgaggtcgc cgttgaaaat catggtcgag taaaaactca ccgcaccgct cggcaaccgt
     2281 ctcgcctgta cttggaaccg cgtaattcca tcggtgagga gggacttgac ttcccccttt
     2341 tctccaaaac catatgttcc ctttttcccc gtggtcat
//
LOCUS       DAOQGJ010000456         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29019_length_2377_cov_3.189061, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000456 DAOQGJ010000000
VERSION     DAOQGJ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29019_length_2377_cov_3.189061"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PK683_08220"
     CDS             <1..525
                     /locus_tag="PK683_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HNH08474.1"
                     /translation="INTLKSLYSAPNYTKLRKSMNLGTITGYRNIIEIADSKSFADLQ
                     NVTAARSDLSASMDMAPDDPEVMRVTSQIIRIGKERGKNTSVGGTITKSNFYTIAGQI
                     SPDVINSRHIAVSLSTIEEKKLSDVPDAMLFFEIELYDLLSHFKPERAFYYQNRISIN
                     RERLGEKKVVYSIR"
     gene            736..>2377
                     /locus_tag="PK683_08225"
     CDS             736..>2377
                     /locus_tag="PK683_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH08475.1"
                     /translation="MFATEGFEAETKIHIGSDVEIYRGRSTADGQPVIIKYIPVSDEL
                     SHSIINIKNEYEIINYLNSNAISRIFRFQREQKGFVLVLEDIGGVSLKDYIQIKPFNL
                     EIYLKICIRLADILSEIHKKKVIHKDIKSDNIIFNEETSDCRFIDFGISTRLSKEETS
                     ASPLGILEGSLQYISPEQTGRMNRALDYRTDFYSLGVTFYELFTGKLPFESEDPLELI
                     HYHLAKNPAAPAEINPNIPEQVSKIILKLMEKTAEDRYQTAFGLKYDLEKCLDQLKKN
                     SAVFSFALGEKDISDEFRIPQKLYGRDRDIESLLNEFKTVLHTGHTKALLIGGYSGAG
                     KSSLIREINKPITEYKGYFLTGKYDQYNRNMPFSAIIQIFSSMVKIILADTPEEIAKW
                     KIRILNAVGKNGKVVTDVIPELEFIIGQQPELPELGPAEEANRFNLVFQKFVSVLASK
                     ERPIAIFLDDLQWADSASLGFIKILLTEPTVKSIFLIFAYRDNETDDSHPFIIMLDEL
                     KKENIKPEKITLKSLSQDHIQELLSDTFRTESESVIEFA"
BASE COUNT          795 a          480 c          476 g          626 t
ORIGIN      
        1 ataaatacac tgaaaagctt atactctgcc ccaaattaca caaaacttcg aaagtctatg
       61 aatctgggaa caattaccgg atacagaaat atcattgaaa ttgcagactc aaaatcattt
      121 gcagatcttc agaacgtgac agctgcaaga tccgatcttt ccgcatccat ggatatggca
      181 cctgatgatc ctgaggtaat gagagtcact tcgcagatta tcagaatcgg aaaggaaaga
      241 ggaaaaaaca catcagtggg cggtacaatc accaaaagca atttttatac aattgcaggt
      301 cagatatccc ccgatgtcat caactccaga catatcgcag tcagcctgag cacaattgaa
      361 gaaaagaagc tcagtgatgt tccggatgca atgctttttt ttgaaataga actttatgat
      421 cttctttctc atttcaaacc cgaaagagca ttctactacc agaacagaat cagcatcaac
      481 agagaaagac ttggagaaaa gaaagtcgta tactctatcc gttaaacact gattaataat
      541 attttcattt gaaatgaaaa ttgcttgtag aattcccctc tgtgaaaatt ctatttatgg
      601 ctatgcatga gactggagct gggagtgcat aatctgtaaa tcatttacgg aaggcaggat
      661 tttcagaaga ctcttcctcg atgatatcat tcaggtttcc agtaagttcg tctgcaggtc
      721 ataaggtgtc tagggatgtt cgcaacagag ggattcgaag cagaaacaaa aattcatatc
      781 ggctcagatg tagaaatcta cagagggcgc tccacagccg acggtcagcc tgtaatcatc
      841 aaatatattc cggtaagtga cgaactgagt cacagtatta tcaatataaa gaacgaatat
      901 gaaatcatca actatctgaa ctcaaatgca atatccagaa ttttcagatt ccagagagaa
      961 cagaaaggat tcgtattggt tcttgaggat atcgggggag tatcactgaa ggattatatt
     1021 cagattaagc cgttcaatct ggaaatatat ctgaaaatct gcataagact tgcagacatc
     1081 ctttcagaaa ttcataagaa aaaagtcatc cataaagaca tcaagtcgga caatatcatt
     1141 tttaatgaag agacctctga ctgcagattt atagacttcg gtatttctac caggctctcc
     1201 aaggaagaga cttctgcttc ccctctggga attctggaag gaagtctgca gtatatttct
     1261 cctgagcaga caggaagaat gaaccgtgct ctggactaca gaacagattt ttattctctg
     1321 ggagtgacct tttatgaact tttcacagga aagcttccct ttgaaagtga ggatcctctt
     1381 gaactgattc attatcatct ggcaaaaaat cccgctgctc ctgcagaaat caatccgaat
     1441 attcctgagc aagtcagcaa gatcatcctg aaactgatgg aaaaaacagc agaggacaga
     1501 tatcagactg cttttggact caaatatgat ctggagaaat gcctcgatca gctgaagaaa
     1561 aattctgctg tgttttcatt cgctctcggt gaaaaagata tttcagatga attccgcatc
     1621 cctcagaaac tgtatggaag agacagggat atagaaagtc ttctgaatga atttaagaca
     1681 gtgcttcaca ccggacatac aaaagcactt ctgataggcg gttactcagg tgcaggaaaa
     1741 tccagtctga tccgtgaaat aaacaaaccg atcactgaat acaaaggata ctttctcaca
     1801 ggaaagtatg atcagtacaa caggaatatg cctttcagtg caatcataca gattttttcg
     1861 agtatggtga aaatcattct ggcagacact ccggaagaga tagcaaaatg gaaaatccga
     1921 atactcaatg ctgtcggcaa aaatggaaaa gtcgtcacag atgtcatccc tgagctggaa
     1981 ttcattatcg gtcagcagcc ggaactgcct gagctggggc ctgccgaaga ggccaacagg
     2041 ttcaatctgg tatttcagaa atttgtatca gtacttgcaa gcaaggaaag accaatcgcc
     2101 atttttctgg atgatcttca gtgggcagac agtgcttctc tcggattcat aaaaattctt
     2161 ctgactgaac ctacagtaaa gtccatattt ctcatctttg cctacagaga caatgaaaca
     2221 gatgattcgc atcctttcat tatcatgctc gatgagctga agaaagaaaa tataaaacct
     2281 gaaaaaatca ccctgaaatc gctttctcag gatcatattc aggaactgct ttccgacaca
     2341 ttccgcacag aatctgaatc ggtaattgag tttgcaa
//
LOCUS       DAOQGJ010000457         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29132_length_2371_cov_3.908895, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000457 DAOQGJ010000000
VERSION     DAOQGJ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29132_length_2371_cov_3.908895"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1275
                     /gene="metK"
                     /locus_tag="PK683_08230"
     CDS             115..1275
                     /gene="metK"
                     /locus_tag="PK683_08230"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNH08476.1"
                     /translation="MSLKDYIFTSESVSEGHPDKVCDQISDAILDAFLAQDLKSRVAC
                     ETLVTTNLVVIAGEITSQGRVDTQEVARDVIRKIGYNDINMYFDADFAVVSSHVHRQS
                     PDIAQGVNEGEGLFKEQGAGDQGLMFGFAIDETPELMPAPIYYSHKLLENLSSLRHTG
                     KLNWLRPDSKSQVTIEYKDNKPVRVDTVVISTQHKPDVTHKQIEEAVIEECIKKIIPA
                     NLLNNTRYFINPTGKFEIGGPHGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDR
                     SAAYMGRYIAKNVVAAGLAKRCEVQLAYAIGVAEPVSVLIDTFGTGTVSDEEISKRVK
                     ANFRLTPRGIIESLKLLEKGRTYQDTAAYGHFGRSGNTFTWEKTDLAEALKK"
     gene            1275..2231
                     /locus_tag="PK683_08235"
     CDS             1275..2231
                     /locus_tag="PK683_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HNH08477.1"
                     /translation="MGAPTTSQTGLKATRDGYGDALAELGAEKPDVIVLDADLSGSTK
                     TNVFKKKFPERFINVGVAEQNLVGHAAGLALTGYTPFASSFAMFLSGRAWEVVRNSVA
                     YPKLNVKLVASHGGITVGEDGASHQCIEDFGIMRVIPEMTVICPSDYEETKQVVKRIY
                     EYKGPVYVRVGRPNIPILSRPADYHFEIGKAELRRRGNKAVIIACGVMVTEADTAAEI
                     LAAKGIEVSVINMATIKPIDEKAILEEAKRTGLVITCEEHNIKGGLGSAVSEYLSEVH
                     PTKVIRMGMKDEFGKSGTWQALMDYFGLRAKNIAETVEKHLS"
BASE COUNT          745 a          490 c          556 g          580 t
ORIGIN      
        1 gtccctgcct tcaacagtga actttctctc attttacctg attgacagtc tgattcctta
       61 aaaaatcctt acaattaggc ctgccctgca ggtattttaa tttgggatag ctgaatgtct
      121 cttaaagatt atatttttac ctccgaatct gtatcagaag gtcaccctga caaggtctgt
      181 gatcagattt ccgatgccat actggatgcc tttctagctc aggatctgaa atccagagta
      241 gcctgtgaaa ctcttgtaac tacaaatttg gtagtcattg caggagaaat tacaagtcaa
      301 ggcagagtgg atacacagga agttgcacgt gatgtaatca gaaagatcgg ttacaatgac
      361 atcaatatgt atttcgatgc agatttcgcg gtcgtatcat ctcatgttca cagacagagt
      421 cctgacattg ctcagggagt gaatgaaggc gagggacttt ttaaggaaca gggagcaggc
      481 gatcagggac tgatgttcgg ctttgcaatt gatgaaactc cagaactgat gcctgcacca
      541 atctactatt cccataaact tctggaaaat ctctcttcac tgagacacac cggtaagctg
      601 aactggcttc gccctgattc caaatcccag gtgacaattg aatataagga caacaagcct
      661 gtgagagtag acactgtagt catctccacc cagcataagc ctgatgtaac tcacaagcag
      721 atagaagaag cagtcatcga agaatgtatc aaaaaaatca ttcctgcaaa tcttctgaac
      781 aatacaagat atttcatcaa ccctacagga aagtttgaaa tcggcggacc tcacggagat
      841 actggtctca ctggaagaaa aatcatcgta gacacatacg gcgggatggg acgtcacggt
      901 gggggagctt tctcaggaaa agatccttcc aaagtggaca gatccgctgc ctatatggga
      961 agatacattg ctaaaaacgt agtggctgca ggtcttgcaa aaagatgtga agtgcagctg
     1021 gcatatgcaa tcggcgtggc tgagccagtg tccgtactca ttgacacttt cggcacagga
     1081 acagtttctg atgaagaaat ctccaaaaga gtaaaggcaa atttcagact gactccaaga
     1141 ggaatcattg agtctctgaa gcttctggaa aaaggcagaa catatcagga tactgctgca
     1201 tatgggcatt tcggaagatc aggaaacact ttcacttggg aaaaaaccga tctggcagag
     1261 gcactgaaaa aataatggga gctcctacaa cttcacagac tggattgaaa gcaaccagag
     1321 acggatacgg tgatgcactc gctgaattag gtgcagaaaa gcctgatgtc atcgttctag
     1381 atgcagacct ttccgggtct acaaagacaa atgttttcaa aaagaaattt ccggaacgtt
     1441 tcatcaatgt gggagttgca gagcagaatc ttgtcggtca tgctgcaggg ctggctctga
     1501 ctggatacac tccttttgcc tcttcctttg ccatgtttct gtccggcaga gcctgggaag
     1561 ttgtaagaaa cagtgtcgcc taccctaaac tgaatgtgaa gctggtggcc tcacacggcg
     1621 ggatcacggt aggagaggac ggtgcatccc atcagtgcat tgaggatttc ggaattatga
     1681 gagtgattcc tgaaatgaca gtcatctgtc cttccgatta cgaagaaacc aagcaggtag
     1741 tcaaaagaat ctatgaatac aaaggtccag tctatgtccg tgtaggaaga cctaatattc
     1801 cgatcctttc gagacctgct gactatcatt ttgaaatcgg taaagcagag ctgagacgca
     1861 gaggcaacaa agctgtgatc attgcctgcg gagtaatggt tacagaagca gacactgcag
     1921 cggaaattct tgcggcaaaa ggaattgaag tgagcgtcat caatatggct acaatcaagc
     1981 caattgatga aaaggcaatt ctggaagaag ccaaaagaac ggggcttgtc atcacctgcg
     2041 aagaacacaa tatcaagggc ggactcggtt ctgcagtttc cgaatatctt tctgaagtac
     2101 atcctacaaa ggtcattcgg atgggaatga aggatgaatt tggaaaatca ggaacttggc
     2161 aggcattgat ggactacttc ggactcagag caaagaacat cgctgaaaca gtggaaaaac
     2221 atctcagctg aacgcggact gcaaaaaaag agaagtctta tctttcagga tagggcttca
     2281 tttttttaaa gaaagggaaa agaaataaac attgaatgac taatccctgt attaaaaaca
     2341 aataaaatat cactccgata gacagccttt t
//
LOCUS       DAOQGJ010000458         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29179_length_2369_cov_3.557476, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000458 DAOQGJ010000000
VERSION     DAOQGJ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29179_length_2369_cov_3.557476"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..1617
                     /locus_tag="PK683_08240"
     CDS             352..1617
                     /locus_tag="PK683_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNH08478.1"
                     /translation="MKNSTQDWEHLLKEGTVLPFFQPVISLESKSVAGYECLGRLDLD
                     GKLMSLGNFFQAKSLQRQGSRLRDSEAKKWKMQIDSTVRNSAIEKFALWNDSSLKLFI
                     NISPAFLAEELSNLQDYTSEFMKLTDSFGVDPQNIVIEITEDAVEQSLDDFKPLINLY
                     KRKGYIIAIDDLGKNSSNLDRIALFRPDIVKVDMHMLQRALEDQSYQEILHSLARLTQ
                     NLGISLLYEGVENSAELLMAMGHGSRYLQGYFFSEPVHDFIDRDHYGRHLESGLSRFM
                     ENISQKIADKVGWEKRILDILSQMHIDTYSVERNFPELEKIFRTDASIFRFFVTDLFG
                     NQISPNFVCRTDFGIETDYSSLKTNWCWRPYFINHVYHSRIQNESWIISEAYHDIREE
                     KILRTFSRTIEGKYYIFIDVDYGLSSGFE"
     gene            complement(1590..2255)
                     /locus_tag="PK683_08245"
     CDS             complement(1590..2255)
                     /locus_tag="PK683_08245"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HNH08479.1"
                     /translation="MIPKFFPIISLILFSSNLSAAGLGSTGAFDFLIIALLLILGGFI
                     FYLQSQVQKKRRETEELLEKLALKERAWTPDITKDYISKVFHLVQKSWAEKNADVAKD
                     FMSESLYLNHKVQTDLMVSNHEKNMLERIRLKEIHTAEILDYQDAKRDIMLVLIKGSM
                     IDYVIDEITGKIKWGKKDEPEEISELWKFIRSRDRWVLDSIEQKVEIKFLHELHSKPE
                     ESP"
BASE COUNT          691 a          445 c          498 g          735 t
ORIGIN      
        1 gcctgcgtgg aggcagggaa ggaatttctg agaaaatctc ctcgaatgta tcactgaacg
       61 attctgcctg acggtcttcg cattatgaac cgatttgcag actgattatt gctgataagc
      121 attctgtttc agagttgaaa gcggattgga gactgtggct aatgcagttg ataaaaacag
      181 cgattcagga cagactgatt tactattttg cctctgaatt cgtgaaattc ggtattagta
      241 aggttcattt tgaattggaa aaggcgaaat ttaattttcg cagttttgaa actcagcgaa
      301 atctccatgt tggcataaaa cttgctttat ggtgactggg ggatttgtat tatgaagaat
      361 tcaactcaag actgggaaca tcttctgaag gaaggaactg tgcttccttt tttccagcca
      421 gtcatttccc tcgaaagcaa gtctgtggcg ggttatgaat gtctgggacg tctggatctg
      481 gacggaaagc tgatgagtct ggggaacttc tttcaggcaa agtcactcca gagacagggg
      541 agccgcttaa gagattctga ggccaaaaaa tggaagatgc agattgattc aactgtcagg
      601 aacagtgcaa tagaaaaatt tgcactatgg aatgattctt cattaaaact ttttataaat
      661 atatcccctg catttcttgc tgaagaactg agcaatctgc aggattatac atccgaattt
      721 atgaaactga ctgactcttt cggagtagac cctcagaata tcgtaattga aattacggaa
      781 gatgctgtgg agcagagtct ggacgatttt aaaccactga taaatcttta taaaaggaaa
      841 ggctacatta ttgcaattga tgaccttggt aaaaattcct ccaatctgga cagaattgct
      901 ttatttcgtc ctgacatagt aaaagtggat atgcatatgc ttcagagagc acttgaggat
      961 cagtcttatc aggaaattct gcattctctg gcaagactta ctcagaattt gggaatttct
     1021 cttctgtatg aaggagtgga aaattccgct gaactgctga tggcaatggg gcatgggtca
     1081 agatatcttc agggctattt cttttctgaa cccgtacacg attttataga cagggatcat
     1141 tacggtcggc atctcgaatc cgggttatcc cgttttatgg aaaatatatc ccagaaaata
     1201 gcagataagg tcggatggga gaaacggatc cttgacattc tcagccagat gcatatagat
     1261 acctattcgg tagaaagaaa ttttcctgag cttgaaaaga tatttcgcac ggacgcttcc
     1321 atattcagat tctttgtgac agatcttttt gggaatcaga tttctccgaa ttttgtatgc
     1381 aggactgatt ttggaataga gactgattac tcatctctga aaacgaactg gtgctggaga
     1441 ccttacttta tcaaccatgt gtatcattcc agaattcaaa atgaaagctg gatcatcagt
     1501 gaagcctacc atgatatcag agaagaaaaa atactcagaa cgttttcgag aacaatagag
     1561 gggaaatact atatttttat tgatgtagat tacggacttt cttccggttt cgagtgaagt
     1621 tcatgcagga attttatttc tactttctgt tcgatactgt ctaaaaccca cctgtctctg
     1681 cttctgataa atttccaaag ttcactgatt tcttccggtt catccttttt gccccattta
     1741 attttaccgg tgatttcgtc gatgacatag tcaatcatgc ttcccttgat cagaacgagc
     1801 ataatatctc tcttagcatc ctgataatcc agaatctctg ccgtgtgaat ttccttcagt
     1861 ctaattcttt ccagcatatt tttttcatga ttgctgacca tcagatcagt ctgcacttta
     1921 tgattcagat acagggattc agacataaag tctttcgcaa cgtctgcgtt tttttccgcc
     1981 caggatttct gcactagatg aaatactttt gaaatatagt cttttgtgat atctggggtc
     2041 caggctcttt ctttcaaggc aagtttttca agcagttctt ctgtttccct tcttttcttc
     2101 tgtacttgag actgcagata aaaaataaat ccccccagga tcagaagcag agcgataatc
     2161 agaaaatcga atgcacctgt gctgcctaat ccggctgctg ataaattgga ggaaaacaga
     2221 ataagactga taatcgggaa aaacttagga atcattattt ttcaaattac agactgcaga
     2281 gatgttccgc agtctgattc atcaaattaa gctgtgatgg gagaaaatac tgattttttt
     2341 tccagatagt aagccagacc gaagaagac
//
LOCUS       DAOQGJ010000459         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29184_length_2369_cov_2.967589, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000459 DAOQGJ010000000
VERSION     DAOQGJ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29184_length_2369_cov_2.967589"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /locus_tag="PK683_08250"
     CDS             complement(<1..2038)
                     /locus_tag="PK683_08250"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNH08480.1"
                     /translation="MKKRPHLTPEEIESFGKEIKELYLETKSKLGKEDLDLAAHYDNQ
                     SKFLDLAGRTLMGLAGPIGWTAGVGMVAAHYTIEFSQGHNILHGQYDDIPGNEKFLSS
                     SWTWDNTMDEEDWKFEHHTVHHPFTNVIGKDNDFGFIIYRGNSAQEWNPFHLVQLPSI
                     LLQPVINVLFFPWYVSTSRAISEKRELLSWETYGNSLKKLFEHFSKNYILSPILAGAN
                     APKTVLGNLAAKIISDYHLTFILGISHLTVDSDVFTERSEETEGEYYYRQVMGTVNYE
                     VPESTEILYGGINTHLEHHLFPDLPPNRLKELAPKVKAVCEKYGIPYITAPFLEQIPK
                     LLQNILENSLPSKPKDSFPPLSLLFKPFELGKRILEGTERFLNFWGKPIPQFHTDSFL
                     ETVIMERSEDAGSVTHLKIKVPEGWEGISWEPGSYISVMVNISGKDYVRQYSLVKPYA
                     DTDLYEIAVKRIPGGLVSNYIADSLQENDKIIIAGTPKGDFTMRHVPEKILFMGAGIG
                     ITPLLSMLRSLRKSAIFPNVCFLYFNRSVHEISFHSEINRLASDMGIRLIHILENPAD
                     DSVKDRFRPVRYWDGKVNRELLLSAVNDINERHIYICGPAGFMNSVKSILNETGFDFK
                     KYHSENFRPSVPEKDPSREKTFRRIKFIKSDKETIINENTTLLEAIRGTGIRIPS"
BASE COUNT          646 a          484 c          509 g          730 t
ORIGIN      
        1 ccgaaggaat tctgatgcct gtgcctcgaa ttgcttcgag cagagttgta ttttcgttaa
       61 tgatcgtttc tttgtccgat ttgatgaatt tgattcttct gaaagtcttt tcgcgggatg
      121 ggtccttttc cggaacagag ggtctgaagt tttcagaatg gtatttttta aagtcaaacc
      181 ctgtttcatt cagtatggat ttcactgaat tcataaatcc tgccgggccg cagatgtaga
      241 tatgcctttc atttatgtca tttactgcac ttagcagaag ttctctgttg accttcccgt
      301 cccagtatct tacaggcctg aatctgtctt tgactgaatc gtctgcaggg ttttccagaa
      361 tatggatcag tctgatcccc atatccgagg caagcctgtt gatttcactg tgaaaactga
      421 tctcgtgtac ggatctgttg aagtacagga aacatacatt tgggaagatt gcagatttgc
      481 ggaggcttct gagcatgctc aggagaggag tgattcctat gccggcaccc ataaaaagta
      541 ttttttccgg aacatgtctc attgtaaaat ctcccttggg agttcctgct ataatgattt
      601 tgtcattttc ctgaaggctg tctgcaatat aattggatac aagtccgcca ggaatccgtt
      661 ttactgcgat ttcataaaga tctgtatctg cataaggctt tacaagactg tactgtctta
      721 catagtcttt tccgctgata ttgaccatta cagaaatata agaaccgggt tcccaggaaa
      781 ttccttccca gccttcggga acctttattt tcagatgggt cacgctgcct gcatcttcag
      841 atctttccat aatcactgtt tccagaaaag agtctgtatg gaactgagga atgggtttac
      901 cccagaaatt aaggaatcgt tctgttcctt ccagaatcct ctttcccagt tcaaacggct
      961 taaaaaggag actgagagga ggaaaactgt ctttcggctt tgacgggagg gaattttcca
     1021 gaatattctg aagaagtttc ggtatctgtt caaggaaagg agcagtgata taaggtatcc
     1081 catatttttc gcagacagcc ttcactttcg gggcaagttc cttgagtctg tttgggggga
     1141 gatcagggaa aagatgatgt tccagatgtg tattgattcc gccgtacagt atctctgttg
     1201 attcaggcac ctcatagttg actgtaccca taacctgcct gtaatagtat tccccttcag
     1261 tttcttcaga tctttcagta aatacatctg aatccacagt cagatgagaa atgcctaata
     1321 tgaaagtaag atgatagtca gaaataattt tcgctgcaag atttccgagc acagtcttag
     1381 gcgcattagc tcctgctaat ataggtgaaa gaatataatt ctttgaaaaa tgctcaaaaa
     1441 gcttcttcag tgaatttccg taagtttccc agctcagaag ctctcttttt tccgaaattg
     1501 ctctggatgt ggaaacatac caggggaaga aaagtacatt gatgaccggc tgaagcagga
     1561 tggacgggag ttgaaccaga tgaaacggat tccattcctg tgccgaattt ccacggtata
     1621 taatgaatcc aaaatcgttg tcttttccga tcacatttgt aaacggatga tgaaccgtat
     1681 ggtgctcaaa tttccagtct tcctcatcca tggtattgtc ccaggtccat gatgaggaga
     1741 gaaatttttc gtttccaggg atatcatcat actgaccgtg aagaatatta tggccttgag
     1801 aaaattcaat tgtataatgt gcagccacca taccgacgcc tgcagtccat ccaatcggtc
     1861 cggccagacc catcagagtt cttcccgcaa gatccaaaaa ttttgattgg ttgtcataat
     1921 gcgctgcaag atccagatcc tctttcccca gtttcgattt tgtttccaga tacagctctt
     1981 tgatttcttt tccaaatgat tcaatttctt caggggtaag gtggggtctt ttcttcatac
     2041 tgattcattt tccttttccg ggaaaaataa caagcgtttt ttttaaatct ctattatgat
     2101 agggaagtaa attacgggga agaaaagaga aaatgagtat gtaaaaattg tcaatgcaga
     2161 cggccgggca gtcggaatca tcctgctgca aatcggtcag acaaacagtc cggacaggaa
     2221 taaaatcctg actcatcttt tgcggatatg tatttaaaaa accatattgg aaaaacagca
     2281 gcagctttca gactggcagc taacttcata aggtttggca gctaacttca taaggtttgg
     2341 cagctaactt cataaggttt agcgagggc
//
LOCUS       DAOQGJ010000460         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29187_length_2369_cov_2.746759, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000460 DAOQGJ010000000
VERSION     DAOQGJ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29187_length_2369_cov_2.746759"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PK683_08255"
     CDS             <1..257
                     /locus_tag="PK683_08255"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HNH08481.1"
                     /translation="RFSLKIDDYRNNEKIFTSAYSGRRKPFSDSEILKRFLLHPLMTL
                     RVIALIHWQALILYLKKIKFYRKNEKLELQKGVYLGKDYS"
     gene            238..1539
                     /locus_tag="PK683_08260"
     CDS             238..1539
                     /locus_tag="PK683_08260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNH08482.1"
                     /translation="MEKTILESVSAEKENSAPVSFGWTDRLFRSVFIKALSKMQKGSV
                     RVIDPFCGNYFLGNTESPQNPEFHSGMIRVNSKDFYRKAVLFGDIGFSEGYLDGDWDT
                     DNIIHVLNWFLLNVETNPSVSGTKKFRPHIRFFNFLNRLYHLKRDNTVRGSRKNIIEH
                     YDLGNSFYRLFLDRTMTYSSAYFNRENLSLEEAQAEKYKALADRLFISRDMHILEIGS
                     GWGGFSLYLAKRFGCRITTVTISDEQYAYSKALFEREGVSSLIDIQLKDYRHIEGRFD
                     RIVSIEMLEAVGHRHLETYFRKCSDLLNPDGLIGLQVITSPDSRYEEFRTGVDFIQKH
                     IFPGSLLPSIGRINEAVNKTGTMHMYSLLDMGMSYAKTLRLWLKGFEENLSQVYSLGF
                     DERFARLWRYYLSYCAAAFEMRNISVMQMVYTRPNNRTLIS"
     gene            1536..1865
                     /locus_tag="PK683_08265"
     CDS             1536..1865
                     /locus_tag="PK683_08265"
                     /inference="COORDINATES: protein motif:HMM:NF018991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HNH08483.1"
                     /translation="MKYLKIFMIFVFVFMLVNTVKASLELNLFSHLPVLIRDPWAVMT
                     LYDAYFAFLFFYAWVIYKEESVILRILWLLLILTTGTMAMSAYVLKEIFKLKDGEGVS
                     ELLLRRK"
     gene            1862..>2369
                     /locus_tag="PK683_08270"
     CDS             1862..>2369
                     /locus_tag="PK683_08270"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNH08484.1"
                     /translation="MKLFLKRKLVDPLLDLLKQGITPEKLAVSAVLGCILGVFPVLGS
                     TVILCTAAAYVFRLNFAAIQTVNYLVYPLQLALFIPFIRVGEFILNSEPFPISMQEIF
                     KLLQQDIILAIQTFWTANLHGIFAWTVICPVSAFAAYFPVKYIFVKAGKKLKEKSNGP
                     CKVCCTYPG"
BASE COUNT          672 a          467 c          529 g          701 t
ORIGIN      
        1 acagattctc tctgaaaata gatgactata gaaataatga aaaaatattc acttcagcat
       61 actcaggcag aagaaaaccc ttcagtgaca gtgaaatcct gaaaagattt cttcttcatc
      121 ctctgatgac actccgagtc atcgccctta ttcactggca ggccctgatt ctatatttaa
      181 agaaaattaa attttacaga aaaaacgaga agcttgagct tcagaaagga gtttaccttg
      241 gaaaagacta ttcttgaatc agtcagtgca gaaaaagaaa attctgcacc tgtttctttc
      301 ggatggacag acagactgtt cagatcggtg ttcataaagg ctctgtccaa aatgcagaaa
      361 ggttcggtca gagtcattga tcctttctgc ggaaattact ttttgggaaa tacagagagc
      421 ccgcagaatc ctgaatttca ttcagggatg atcagagtca acagtaagga tttttacaga
      481 aaagcagttc ttttcggaga tatcggtttt tctgaaggtt atctggacgg cgactgggat
      541 accgacaata tcattcatgt actgaactgg tttcttctga atgtagagac caatccgtcc
      601 gtttccggaa cgaaaaaatt ccggccccat ataaggtttt tcaatttcct gaacagactg
      661 tatcatctga aaagagacaa tacagtaaga ggaagcagaa aaaacattat agagcattat
      721 gatctcggca acagtttcta caggcttttc ctggacagaa caatgacata ttcatctgca
      781 tatttcaacc gggagaatct gagtcttgaa gaagcccagg cagaaaaata caaggctctt
      841 gctgacagac tctttatcag cagggatatg catattctgg aaataggaag cgggtggggc
      901 gggttcagtc tttatctggc aaagagattc ggatgcagaa tcactacagt cactatctcc
      961 gatgaacagt atgcttattc gaaagcactt tttgaaaggg agggagtgtc atcgctgatc
     1021 gatattcaac tgaaggacta caggcatatt gaaggcaggt ttgacaggat tgtatcgata
     1081 gaaatgcttg aagcagtagg acaccgtcat ttggaaacat atttcagaaa gtgttccgat
     1141 cttctgaatc cggacggtct cattggactg caggtgatca cttctcccga ttccagatat
     1201 gaagaattca ggacaggtgt tgattttata cagaagcata ttttcccggg ctcacttctt
     1261 ccatcgatag gcaggataaa tgaagctgtg aataagaccg gaacaatgca tatgtacagt
     1321 cttctcgata tgggaatgag ttatgcaaag acactgcgtc tctggcttaa gggttttgaa
     1381 gaaaatctgt ctcaggtgta ttcccttgga tttgatgaac ggtttgcaag actctggaga
     1441 tattatcttt cctactgtgc agcggctttt gagatgagaa acatcagtgt catgcagatg
     1501 gtttacacaa ggcctaacaa caggactctg atatcatgaa atatttaaaa atattcatga
     1561 tatttgtatt tgttttcatg ctggtgaaca cggtgaaggc ttctctggaa ctgaatcttt
     1621 tcagtcatct tcctgtcctt atccgtgacc cctgggcagt aatgacactg tatgatgcgt
     1681 attttgcctt tctgttcttt tatgcctggg tgatttacaa ggaggagagt gtcattttgc
     1741 gcattctctg gcttctgctc atcctgacga caggcacaat ggcaatgtcg gcatatgttc
     1801 tgaaggaaat ttttaaactg aaggacggtg agggagtctc ggagctgctc ttgaggagaa
     1861 aatgaaactt ttccttaaaa gaaaactggt tgatcctctg cttgaccttc tgaaacaggg
     1921 gatcactcct gaaaagcttg cagtgtctgc cgttctcggg tgcatacttg gagtctttcc
     1981 tgtcctcggt tcaactgtga ttttatgtac agcggctgcc tatgtcttcc ggctgaactt
     2041 tgctgcaatc cagacagtga attatctggt gtatcctctt cagcttgctc tgtttattcc
     2101 gtttatcagg gtcggagagt tcattctgaa ttcggaaccg tttcctatca gtatgcagga
     2161 aattttcaag cttcttcagc aggacattat tcttgcaatt cagactttct ggactgcaaa
     2221 tctgcacgga atctttgcct ggacagtcat ctgtcctgta tcggcatttg ctgcctattt
     2281 tccggtaaaa tatatttttg taaaagcggg aaaaaaactc aaggagaaat caaatggccc
     2341 gtgcaaagtc tgttgtactt accccggct
//
LOCUS       DAOQGJ010000461         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29285_length_2364_cov_2.911650, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000461 DAOQGJ010000000
VERSION     DAOQGJ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29285_length_2364_cov_2.911650"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..802
                     /locus_tag="PK683_08275"
     CDS             236..802
                     /locus_tag="PK683_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08485.1"
                     /translation="MKTLLISILLFAAPLFSEEKRIFKKMDSIPSESSCKSDHFTVCL
                     AYKFLKHFYPELKNPEWYRNTVPSHYFEYKVVYNNSAENGEIIFSFESFSDGLEWGYW
                     DDSVISRTTSPIQIIESEKTRQMIQKALEKKFGKDLQIEKITKIIERRCMQSQTNPKD
                     YYDIKENIRFKVQIKGESVLFKQNGEMR"
     gene            complement(826..1509)
                     /locus_tag="PK683_08280"
     CDS             complement(826..1509)
                     /locus_tag="PK683_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08486.1"
                     /translation="MKNWLMTVCSVMICVAVNISAKPKNIKIECGNSDSDAPALKKAP
                     GNVKRLLKGNVLTVTLEIEGRSLNFVYKNSEDNKGELDGLKWQYCGYRKDMKLHLIKK
                     EEDGYFSGKLLDENIGEDFEAGYRIIISEDGDTIFAARQPNGMDGEEWFLLDRYGKVL
                     WKGESVFTDKQKSITAELESPDFDKDGNLTAVQKCTAEKAKKKTAKIILKKKDKKFQW
                     ESRISCPSA"
     gene            complement(1506..1940)
                     /locus_tag="PK683_08285"
     CDS             complement(1506..1940)
                     /locus_tag="PK683_08285"
                     /inference="COORDINATES: protein motif:HMM:NF036886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5618 family protein"
                     /protein_id="HNH08487.1"
                     /translation="MKASKAKKIRHSASSKEDAISEAYRYLKNAKETISKSSIELGIR
                     YTDSKYVREAAGIAYLAPLLAIDGYLLGRGETSANLPNSIEEYFTVLEKIPSNKRLKA
                     SLHVVYENLHILAYYRGGVGVEMVKEGFQRAKDIVEELENKK"
     gene            2095..>2364
                     /locus_tag="PK683_08290"
     CDS             2095..>2364
                     /locus_tag="PK683_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08488.1"
                     /translation="MSVMETYFKTMFFILPFMMSFDVFIGKSFFTGDSFFQDYPMRHY
                     LGQSILNLDFPFYAFEGNFGFPFFAEISSGTLYPLNWFFSVIAFKF"
BASE COUNT          641 a          514 c          427 g          782 t
ORIGIN      
        1 atttgaatct ggggacacca tcgatttgtt ttgccgcctc cgacctgccg gaatttagaa
       61 ataaattgat aaaaaacggc gtgcagacaa cggaaattgc cgaccatttc ggatttgaga
      121 gtttcgcatt cagtgacaac gacggaagct ggtttgctgc aatgaagcag taaacagccg
      181 actattctca gaattggaaa ctgatcggta aaaatccaat aatataggtt actttatgaa
      241 aactcttctc atttcaatac tgctttttgc ggctcctctg ttttctgagg aaaaaagaat
      301 cttcaaaaaa atggattcta taccgagtga atcttcctgc aaatcggatc attttactgt
      361 ctgccttgca tataaattcc tgaaacattt ttatccggaa ttaaagaatc cggaatggta
      421 caggaacact gtaccgtcgc attattttga atataaggtc gtttataata attcagcgga
      481 gaacggagaa atcatttttt catttgaatc tttttcggac ggactggaat ggggctactg
      541 ggatgattcc gtaatcagca gaacaacgtc acctattcag atcattgaat cggaaaaaac
      601 acggcaaatg attcagaagg ctctggaaaa aaaattcggc aaggatttgc aaattgaaaa
      661 aatcacaaag attattgagc gcagatgtat gcagagtcag acaaatccca aggattatta
      721 tgatattaag gaaaacatcc gtttcaaggt tcagatcaag ggtgaatcag tgcttttcaa
      781 acagaacggt gaaatgcgtt aaacttcatt ctctttttag agcttttatg ctgaagggca
      841 gctgattctg gattcccact gaaacttctt atccttcttt ttcagaatga tttttgctgt
      901 tttctttttt gccttctctg ctgtgcattt ctgaactgca gtcaaatttc catccttatc
      961 aaagtcagga gattccagtt ctgcagtaat ggacttttgt ttatccgtaa atacagactc
     1021 tcctttccat agaaccttcc cgtacctgtc taagagaaac cattcttctc cgtccattcc
     1081 gttcggctgt cgggcggcaa atatcgtatc accgtcctcc gatataatga ttctatatcc
     1141 tgcttcaaaa tcttctccga tgttttcatc cagcagtttt ccggagaaat atccgtcttc
     1201 ttcttttttg atcagatgca gtttcatatc cttccggtag ccgcagtact gccatttcaa
     1261 accgtccagc tcgcctttat tgtcctctga gtttttatag acaaaattca agcttcgacc
     1321 ctcaatttca agagtcactg tcagaacatt ccccttgagc agtcttttca cattgccggg
     1381 ggcttttttc aatgcaggag catccgaatc agaattgccg cattctattt ttatattctt
     1441 aggtttcgct gaaatattta ccgcaacgca tatcatcaca gaacagacag tcataagcca
     1501 gttcttcatt ttttattctc cagttcctct acaatatcct ttgctctctg aaatccttcc
     1561 ttgaccattt cgacaccgac acctcctctg taatatgcca gaatatgaag attctcatat
     1621 acaacgtgca ggctggcttt cagcctttta ttggaaggga ttttttcgag aactgtaaaa
     1681 tactcttcaa tggaatttgg aagatttgcg gaagtttctc cgcgaccaag cagataaccg
     1741 tcaattgcca gaagaggcgc cagataagca atccccgctg cttcccttac atacttggaa
     1801 tcagtatatc gaattccgag ttcaattgaa cttttagaaa tagtctcttt tgcattcttg
     1861 agataacggt acgcttcaga aattgcgtct tccttgctgg aggctgaatg tcgaattttc
     1921 ttcgccttgg atgctttcat agagtctcag tttaaaattc tcctttgaaa atacctgtca
     1981 agaaaataat ccaaaccgtt atttttccga agaagtttac tcttttcgct caattttttt
     2041 tctaagacga accgattctt ttgttttcag atcaaatcat tctgcagaat ctgtatgtca
     2101 gtcatggaaa cctacttcaa gacaatgttc ttcattctcc cgtttatgat gtccttcgat
     2161 gtgttcatcg gaaaatcctt ttttacaggg gattcttttt ttcaggacta tccaatgagg
     2221 cattatctgg gtcagtcaat actgaacctg gactttccat tttatgcttt tgagggcaat
     2281 ttcggatttc ccttttttgc ggaaatctcc tccggcacac tttatcctct gaactggttt
     2341 ttttccgtca ttgcattcaa gttc
//
LOCUS       DAOQGJ010000462         2362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29325_length_2362_cov_3.250542, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000462 DAOQGJ010000000
VERSION     DAOQGJ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29325_length_2362_cov_3.250542"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1384
                     /locus_tag="PK683_08295"
                     /pseudo
     CDS             54..1384
                     /locus_tag="PK683_08295"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
     gene            1381..>2362
                     /locus_tag="PK683_08300"
     CDS             1381..>2362
                     /locus_tag="PK683_08300"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNH08489.1"
                     /translation="MMIYRFTIIIIISFLYECRHSENSSKGSGLDGLIYMNMNSFNTE
                     KMLSDTVENIILPEYRNLDRLASVFETSAGAYASGLNTAQLETLRSDWRNLQSALKNA
                     ELFNFGPAGIPDNYYIRLDFSAISGGAKLDVSGMNTVIEGSSPSSVSQNYIAGQGTKR
                     GLIAAEKLLFDDTVYPEDSVFPDSASAVSSAFSSKQRRLDFLRSLSLDIKDTASKLIR
                     AWEPSGGNYIQVFLGKTPDSKYFIKTDAVSELINQSVFLLEAMTDRKIGLPAGLSSSS
                     KGIKNSLLVESKYSDNSLPDLLNNIQALRKLYTGKGRENSGSGISDYIKYY"
BASE COUNT          689 a          489 c          558 g          626 t
ORIGIN      
        1 gaagacagac cacagctgct gccgcaattg ctctgggtgc ttccgcagac gatctgggtg
       61 aggaatattc tgcgggaaat ctcactgtat ttgacgattc atccaatgca ttcaacagtc
      121 caggctctaa tcttaaagga cagagagtga ttgatttcaa taccggcaat gctgtctttg
      181 attctacttg gatacctgca ggaaacagtg tactttcagg aagaggtcct ctctggaaca
      241 gttcctcctg caggggatgt cacgtttttg acggaagagg aagacctccg aagcaggaag
      301 aaactgagct gacatcaatg ctgatccggc tcagtatacc cggtacggat gcaaatggag
      361 gtcctttggg agttcccgga tacggcactc agctgaatac cagatacaat gctggaaaat
      421 ctctgacctc tgctgaggga agcgtgagta tcacttatac tgaagtcccc ggaagttttc
      481 ctgacgggga aaaatattcc cttcgtagac caaattataa tattacttat aattttaatt
      541 ctgtgccgtc caatctgatg atttccccga gaacagcacc tatgattccc ggacttggtc
      601 ttcttgaggc cataccggaa agtgacattc ttgcgaatgc agatgaaaag gactctgacg
      661 aaaacggcat ttccgggaga ccgaattacg tgtatgatgt tctttccgga aaaaaacttc
      721 tgggcagatt cggatggaag gcaaacaatc ccagtctgag gcagcagaat caggggcatt
      781 tctaggagat atgggaatta cttccccgct ctttccttca gagggatgtg aatctgcttc
      841 gactcagtat tcagcctgcg tttcggaagg aagcggaaac aacggacatt ctgaaggaac
      901 agaaataaat aaattatctg ttgatttcat tactttttat acacagacag tcggagttcc
      961 ggccagaagg aactggaaca gtgctgatgt aaagaatgga aaaaaactgt tttctgagat
     1021 cggatgtact tactgccata tccccaagtt tacaacagga aaagtgaagg actttccgga
     1081 gatatccgga cagacaatac gtccatatac ggatcttctt ctccatgata tgggagagga
     1141 tctttccgac ggacgtcctg attttctggc ggaaggaaaa gaatggagaa cagcaccgct
     1201 ctggggaatc gggctcatcc agactgtcaa cggtcacaac aacctgctcc atgacgggag
     1261 ggccagagga tttcaggaag ctgttctatg gcacggagga gaagcagaga aatccagaga
     1321 aaaatacaaa tcgctctcaa aggcagacag aaattctgtc ttgaaattcc tggagtctct
     1381 atgatgatat atagatttac tataataatt ataatatcat ttctgtatga atgcaggcat
     1441 tctgaaaatt cttccaaagg ttcaggactt gacggactca tatatatgaa tatgaattct
     1501 ttcaatactg aaaaaatgct ttctgataca gtggaaaaca tcattctgcc tgagtatcga
     1561 aatctggaca gacttgcttc tgtcttcgaa acatctgcgg gggcctatgc ttcaggtctg
     1621 aatactgctc agctcgaaac cttgagatcc gactggagaa atcttcagtc tgcactgaag
     1681 aacgctgaac tgttcaattt cggtcctgct gggatacctg ataattatta tatcaggctg
     1741 gatttttctg caatttccgg aggagcgaaa ctggatgtct cagggatgaa tactgtgatt
     1801 gagggaagca gtccttcttc tgtttcgcag aattatattg caggtcaggg aacaaaacgc
     1861 ggactgattg ctgccgagaa acttctgttt gatgataccg tgtatcccga ggattccgtt
     1921 tttcctgaca gtgcatctgc tgtcagctct gctttttcct cgaagcagag aagactggat
     1981 ttcctgagat cgctttctct cgacataaag gacaccgctt cgaaactgat aagggcctgg
     2041 gagccttcgg gaggaaacta tattcaggtc tttctgggga aaacacctga ttcaaagtat
     2101 ttcataaaaa cagatgcggt aagtgaactg atcaaccaga gtgtttttct gctggaagca
     2161 atgacggaca gaaagatagg acttcctgcg ggactcagca gttcttcaaa gggcataaaa
     2221 aattctctgc ttgtcgagtc aaaatacagc gacaattcac ttccggatct tctgaacaat
     2281 attcaggcac ttaggaaact gtatacagga aaaggcagag aaaattcagg ttctggaatt
     2341 tctgattata taaaatatta ta
//
LOCUS       DAOQGJ010000463         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29343_length_2361_cov_2.947095, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000463 DAOQGJ010000000
VERSION     DAOQGJ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29343_length_2361_cov_2.947095"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /locus_tag="PK683_08305"
     CDS             complement(<1..1859)
                     /locus_tag="PK683_08305"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNH08490.1"
                     /translation="MLNIINILIIILFLLQCTQKSSSDLTANILYSAYKTNQVTLTGR
                     ASKGPIKNGNVFVTALRNDGTCNKADRILGTGYTDLNGDFSVPYTKTGYPVCVFVVPN
                     SSGTSKMYDEKADTDIAWTDSNSSLIMTMREPPSIKKSANVMPFGKIGLTRLSALLKG
                     NTDASKNDAAVKQVNKELTIRFGLNTGLSSTSKNASPKASINGDSYPEITDIQADLSS
                     ADDPYAVKMNLIMAGMSYMANQVKDGKTVSDKDIEKIMTAFASDYEDGLFNGKDKNGS
                     SITVSGTNPITFSSNPLTDNLGAAISSFIKEGGKLNSSFNLTASGITSSLAFNDNAVI
                     TLSAALPGLTFSNSQFMLIKGTALPPITPSFSGGTLTSCSISPALPSGLTMNSATCAI
                     SGTPAVTQHLTSYTVSATNSTGTGTSVIGIMIASGTTATVVYGQNGNFTTSTPTVTAT
                     GLSGAGAILFDSNSNAYIADQMGDRVLFYPSGSTTASAVYGTGGSWAAGGGACSASGL
                     NDPIALALDSSNNLYIAENINRRVTVYPSGSTTAAVTVYGETNMTSCGGGTTSALIGN
                     LSSILIDSTGGLYVSDQTGNRILYYPAGSTVPSRVIGQVNYTNSTANAGGATGP"
     gene            2129..>2361
                     /locus_tag="PK683_08310"
     CDS             2129..>2361
                     /locus_tag="PK683_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08491.1"
                     /translation="MKKLLLSLLFICAPLIASDFDSVSDISSGDCKGIASGDKSWCTT
                     NDCKGIASKDKSWCKSSDCKGIASKDKSWCKSR"
BASE COUNT          580 a          503 c          573 g          705 t
ORIGIN      
        1 gggccggtag ccccgcctgc atttgcagtg ctgtttgtat aatttacctg acctatgact
       61 ctggagggaa cagttgagcc cgcaggataa tacagaattc tgtttccggt ctgatccgat
      121 acatagaggc cgcctgttga atcaatcaga atagatgaca gattcccgat taatgcgctg
      181 gttgtccctc ctccgcagct ggtcatattt gtttcaccat agacagttac agcagccgtt
      241 gtagaacctg aaggatacac tgttaccctt ctgtttatat tttctgcaat gtacagatta
      301 ttgctgctgt ctaaggcaag cgcaattgga tcattgagtc cagatgcact gcatgcccca
      361 ccgcctgcag cccagcttcc tcctgttccg taaactgcag atgcagtcgt ggagccggat
      421 gggtaaaaca atactctgtc tcccatctgg tctgctatat aggcattgct gtttgagtca
      481 aatagaatcg cacctgcacc ggaaagtcct gtcgcggtga cagtcggtgt actcgttgta
      541 aaatttccat tctgcccata gactacggtt gctgtcgtgc cgcttgcaat cataatgccg
      601 atgactgagg ttcctgttcc ggtcgagttt gtcgcagaga cagtgtacga tgtgagatgc
      661 tgagtgacag caggagttcc tgaaattgca caggttgctg aattcattgt cagtcctgag
      721 gggagggcag gggagatgga acaggaagtc agagtgcctc cgctgaagga tggtgtgatc
      781 ggcggcaggg ctgtgccttt gatgagcata aactgtgagt ttgagaatgt cagtccaggc
      841 agcgctgcag atagagtaat cactgcatta tcattaaatg ctaatgatga tgttatgccg
      901 gatgcagtca gattgaatga actgttcagt tttcctccct ctttgataaa actggaaatg
      961 gcagccccca gattgtcggt cagcggattg gagctgaagg taatcgggtt agtccctgag
     1021 actgtgatgg aacttccgtt cttgtctttt ccgttgaaga gtccgtcttc atagtcgctg
     1081 gcaaatgctg tcattatttt ttctatatcc ttgtcggata cagtctttcc gtcttttacc
     1141 tgattggcca tataggacat cccggccatg atgaggttca tcttcactgc ataagggtcg
     1201 tctgcactgc tgagatccgc ctgaatgtca gtgatttccg gataagaatc accgttgatc
     1261 gaggctttag gagaagcatt tttggaagtg ctgctgaggc ctgtattcag accgaagcgg
     1321 atggtaagtt ccttgttgac ctgctttact gcagcatcat tcttggaggc atcggtgttt
     1381 cctttcagaa gggcagaaag acgggtcagc ccgatttttc cgaagggcat cacatttgca
     1441 ctttttttaa tggaaggagg ttctctcatt gtcattatca gagaggaatt ggaatcagtc
     1501 caggcgatgt ctgtatctgc cttttcatcg tacattttgg aagtgccgga agaattaggc
     1561 acaacaaaaa cacagacagg atatcctgtt ttagtgtagg ggacagaaaa atctccgttc
     1621 agatcagtgt aacctgttcc cagaattctg tctgctttat tgcaggttcc gtcgtttctc
     1681 agtgcagtga caaatacgtt tccattctta atcggtccct tgcttgccct tccagtcagg
     1741 gtgacttggt ttgtcttata tgcgctgtaa agaatatttg ccgtaagatc agaagatgat
     1801 ttctgtgtac actgcagtaa aaataaaatg ataatcaata tatttataat attcagcatt
     1861 tgcctctcca tgaccaaaat ccttttacag cattcccgtg gtcaatgcat ttttaactgc
     1921 agtctttctg agtttaagac tcagtgatct gacaggcagt ctatgattgg ttattctgat
     1981 ctgcagcaaa tttttaaaat ttgagaactg ctgacgcttt tgcttcgtaa aagaattcag
     2041 acctgttatc tttttgtaat cgctcagccg caggcgtaag gtgagtatgt aaataaaatc
     2101 tttactcagc ggaaacagtc taatgtttat gaaaaaactt ttattgagct tactgtttat
     2161 atgtgctccg ctgattgctt cggattttga ttctgtctca gatatttcca gcggtgactg
     2221 taagggaatt gcatccggcg acaagtcctg gtgcacaaca aatgactgca aggggattgc
     2281 gtctaaggac aagtcctggt gcaagtccag cgactgcaag ggaattgcat cgaaagacaa
     2341 gtcctggtgc aaatccagac a
//
LOCUS       DAOQGJ010000464         2360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29359_length_2360_cov_2.547072, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000464 DAOQGJ010000000
VERSION     DAOQGJ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29359_length_2360_cov_2.547072"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1112)
                     /locus_tag="PK683_08315"
     CDS             complement(153..1112)
                     /locus_tag="PK683_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08492.1"
                     /translation="MPIFLLIALMSMNLSCSSLSRETRMVVHGASYCLSSKIHFEGTA
                     ADQKTAQDFMNDPELNRRFSKKSLRLALAYNISDELKIFTKLEKSARAAKVPNPEFIR
                     VESAIYRRIQLMTMDINAVTSEFGCYVDRFSEIIAQMKQLEDDIVSNNTLYAILSGAV
                     FSFFDGLSVYNNRQDQIIIITGAMVVTYFSYKAFRPEVTIEFKPKSSNLKDIWFKPEK
                     SSNFSTALWMIMTENLIEDDTPIRERLITRWIENGFLGKEDKEDRDFHINLFFGTGGI
                     SDITHITNRREMNSEVKTMILLLSQDINSFLIEVVAGHIQNTE"
     gene            complement(1156..>2360)
                     /locus_tag="PK683_08320"
     CDS             complement(1156..>2360)
                     /locus_tag="PK683_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08493.1"
                     /translation="KNNSVYAKLLCAFGASYSIEAESHHNSEIYYLKAEEFFDKAAAL
                     AEKLNEKEVLAETFHFLALNFRNYMQAYLPNRTISERLPKLKKAVESLEKEYYLLQKT
                     ELSSVPQFNRKRSENLLELALVYRDRWIYETGKPEENIEDYKTGNLKTVLNDYKDHKI
                     SQMAAERAYAYAKENNFYDIAMSCLEIIADSWYITDTVEEKNHTLPKGRSIGLTIANF
                     QLNPKHTDREKAADCYLKAADLAEKNGLIKKSIQYRHQAAMIYSEDMNQQERKKIFTE
                     NINKLEDIYRQSPSSEALNQIYMIKTEMGISYVEKFEKEEEILKYYSSVINDKRFTFH
                     FFLNNHYHMQIYNNPRRRLAELYAEKGKYCEALKWQTEYASFLLMFDPEDIRADLELK
                     EYQTKCSP"
BASE COUNT          610 a          490 c          436 g          824 t
ORIGIN      
        1 caccaggcaa acggtgagtc tttgatgact gcttccagaa atttttcagt ttccattttg
       61 tcccgacctt gctgccgtca cagcgcacta ctgcttatta tgttcagtgc ggaaaaaaag
      121 caatcttttt gagcaccgat tcaccgtgga tttcattcag tattctgaat gtggccggcg
      181 acaacttcga ttaaaaaact gttaatatcc tgagaaagaa gaagaatcat agtcttcact
      241 tcactgttca tttctctcct gttggtaata tgagtgatat cactgatgcc tcctgtcccg
      301 aagaataaat tgatatgaaa atctctgtct tccttatcct cttttcccag aaacccattt
      361 tctatccatc gtgtgataag tctttctctg attggagtgt catcttcaat cagattctca
      421 gtcataatca tccatagggc agtggagaag ttggaggatt tttccggctt aaaccagata
      481 tctttcagat tggatgattt tggcttgaat tcaattgtaa cttcaggtct gaaggcttta
      541 taactgaaat aagtcactac cattgcgcct gtaataatga tgatctgatc ctgccgatta
      601 ttgtaaacgc tcaatccgtc aaaaaaagag aagactgcac ctgaaagtat cgcatataaa
      661 gtattgtttg atactatatc atcttcaagc tgtttcatct gtgcgatgat ctcactgaat
      721 ctgtccacat aacatccgaa ttccgatgtg actgcattga tatccattgt catcagctga
      781 attcttctgt aaattgcgct ttccactctg atgaattccg gatttggcac ctttgcagcc
      841 cttgcacttt tttccagctt tgtgaagatt ttcagttcgt cggaaatatt ataggcaagt
      901 gccaggcgaa gggatttttt ggaaaatctc ctgttcagtt ccggatcatt cataaaatcc
      961 tgtgcagttt tttgatcagc tgctgtccct tcaaaatgta tcttggaaga gaggcagtat
     1021 gatgctccgt gcaccaccat tctggtctcc ctggaaaggc ttgagcagga gagattcatc
     1081 gacatcagtg ctatgagaag aaaaataggc ataaaacaga caggctcttg agtctctctt
     1141 actggcaaac tctttttatg gactgcattt tgtctggtat tccttcagct ccaaatctgc
     1201 cctaatgtct tcaggatcga acatcagcag aaatgatgca tattccgtct gccattttaa
     1261 tgcctcacag tattttcctt tttccgcata caattctgcg agtcttcttc tcggattatt
     1321 ataaatttgc atatgataat gattgttcag aaaaaaatga aaagtaaatc ttttgtcgtt
     1381 tattactgaa ctgtaatact tcagtatttc ctcttctttt tcgaactttt ccacatagct
     1441 gatgcccatt tctgtcttaa tcatataaat ctgattcaat gcttctgaag aaggggactg
     1501 tctgtaaata tcttcgagtt tgttgatgtt ttcagtgaaa attttctttc tttcctgctg
     1561 attcatatct tctgaataaa tcattgcagc ctgatgtctg tattgtatgg atttttttat
     1621 cagcccgttt ttttcggcca gatccgctgc cttaagatag cagtcagcag ctttttctct
     1681 gtccgtgtgt tttgggttca gttgaaaatt tgcgatagtc agacctatgc ttctgccttt
     1741 cggcagagta tggttttttt cttccacagt atcagttata taccaggaat cggcaataat
     1801 ttccaggcaa gacattgcaa tatcataaaa attattctct tttgcatatg catatgccct
     1861 ttcagcggcc atctgggata ttttatgatc tttgtaatcg ttcaatacgg ttttgagatt
     1921 tccggttttg taatcttcaa tattttcttc cggtttccca gtttcataaa tccatctgtc
     1981 acggtaaaca agagcaagct ccagaaggtt ttcggatctt ttcctgttga actgcggtac
     2041 agaggacagt tctgttttct gaagaagata atattccttt tccagtgatt caactgcctt
     2101 tttcagcttt ggcagtcttt cactgattgt ccggtttgga agatatgcct gcatatagtt
     2161 tctgaaattc aaagcaagaa aatgaaacgt ttcagcgagg acttctttct cattcagctt
     2221 ttcggccaaa gcggcggcct tgtcgaaaaa ttcctctgcc tttagataat aaatttcgct
     2281 attatgatga ctttccgctt caatggaata gcttgctcca aatgcgcata aaagctttgc
     2341 atagactgaa ttattcttca
//
LOCUS       DAOQGJ010000465         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29432_length_2356_cov_2.910039, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000465 DAOQGJ010000000
VERSION     DAOQGJ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29432_length_2356_cov_2.910039"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PK683_08325"
     CDS             <1..626
                     /locus_tag="PK683_08325"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH08494.1"
                     /translation="FYSETERYVFFFNGDAMGKSMQGAGGAIVAGTVVNNIIARSARD
                     NKVLHMNPDEWLLNTFREIDEIFCTFDGSMLLSAAAGIIQESTGLLWYSNFEHPRTIL
                     YRDKRADFIDSEKDSTFKFGSMIEGLEYPIMKFQLEKGDVIIVGSDGRDDLDISRSEG
                     RERIINENYSLILAMTEMARGTISGLLEILPEYGDQTDDISFIRISY"
     gene            complement(807..1031)
                     /locus_tag="PK683_08330"
     CDS             complement(807..1031)
                     /locus_tag="PK683_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08495.1"
                     /translation="MNILVGTSSSSSNVGIPSVFAPSLIFAKPLYSGFQGTAFPAAAP
                     ALTENPPVACASSPDLPAGLALAKLFGQII"
     gene            complement(1315..2256)
                     /locus_tag="PK683_08335"
     CDS             complement(1315..2256)
                     /locus_tag="PK683_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08496.1"
                     /translation="MKIYINDELLDSKIENEENLGQVYRQIDEWLQSQGKYILKCLAD
                     GNEKTEKELSSIRLGSAERLDFYAGKTADILLSVLLEMDRYIDTVGNTIFGRDRLTLD
                     EKKHLEEGILWCTEALGTADSMLSIDLSASRVSENGKPVKKILEDLKSAVKSLDSVSS
                     IEKYLNDLRDLKIFVMNLVNKVSLLAVDRNTIAKVITAYSESMETLKEEFIRVNVNFQ
                     SGKDAVAGELLVHSMERLEILLNALISLKAKFHGSDWSVLDEPAEKLHSLMSSVAKSL
                     ESSDIVMAGDLLEYELPGVLDVFTPLLKNLAVRLSSG"
BASE COUNT          707 a          520 c          469 g          660 t
ORIGIN      
        1 ggttttattc cgaaacagag agatatgttt ttttcttcaa cggagatgca atgggcaagt
       61 caatgcaggg agcaggagga gcaattgttg caggaacggt tgtcaacaat atcatagcca
      121 gatctgcaag agacaacaag gttctgcata tgaaccctga tgaatggctt ctgaatacat
      181 tcagggaaat tgatgaaatc ttctgcacat tcgacggatc catgcttctt tctgccgcag
      241 caggaatcat acaggaaagt acaggcctcc tctggtattc caacttcgaa catcccagaa
      301 caattttata cagagacaaa agagctgatt tcattgattc agaaaaagac tccacattca
      361 agttcggaag catgattgaa ggactggaat atcccataat gaaatttcag ctggaaaaag
      421 gcgatgtaat cattgtcggt tcagacggaa gagacgatct ggacatttcc agatccgaag
      481 gaagggaaag aatcatcaat gaaaattaca gtctgatcct tgccatgaca gagatggcca
      541 gaggaacgat atccggactt ctggaaatac ttcctgaata cggggatcag accgatgata
      601 tctcttttat cagaatcagt tactgaaaaa atcagcttca gtatactgtc actctattgt
      661 tcccggtatc ggaaatatac aaatttccga aagaatcaaa ctccagccat tgaggattac
      721 tcagtcctga agccgttaca aataaattcc cgtcggagtc ggacctcctc cgaacgttgt
      781 ctgtgtacag gaagttccgc cggtattcat attatctgtc cgaagagctt agcaagtgca
      841 agcccggcag gcaggtccgg agaagatgca caggcgaccg gcggattttc tgtcagagca
      901 ggcgcagctg caggaaaagc agttccttga aatcccgaat acaacggttt tgcaaaaatg
      961 agagacggag caaaaactga cggaatgccg acgtttgaac tgcttgaaga agtgccgaca
     1021 agaatattca ttctgaagag tgcatctaaa agtacccttt taatggacgg gttgcatcat
     1081 aaaaatacca cagacataaa gcaaagagaa caaatatctt tcgtgaagat tttcataatc
     1141 taagtcattg tgatattttt tttagtcaag cggtaatata aaaaattaat tccgtatcat
     1201 actgacttca attccaactc agtgctcatc atgcagaata aaaaactgcc tataaagatg
     1261 tctctgaaat gaaaggcaat acatagtatc tcagcctgca tcattaaaac cgcatcagcc
     1321 tgaagaaagc ctgacagcga gatttttcaa tagcggtgta aatacgtcaa gaactccagg
     1381 gagctcatac tccagaaggt ctcctgccat tacaatgtca gaactttcca gtgatttcgc
     1441 gacagaactc atcagtgaat gaagcttttc agccggttca tccaggacgc tccagtcgga
     1501 accgtgaaat tttgctttga gtgagatcag tgcattcaga agaatttcca gtctttccat
     1561 cgaatgaacc agaagttctc ctgccaccgc atcctttcct gactgaaaat tgacattgac
     1621 ccggataaat tcttccttga gagtttccat agattcagaa tatgctgtaa ttacctttgc
     1681 aattgtattt ctgtccactg caagcagaga tactttgttt acaagattca ttacaaatat
     1741 tttcagatct ctgagatcgt tcagatactt ttcaatggaa gaaacagaat ccaatgattt
     1801 cactgcggat ttcagatctt ccagtatttt tttgacaggt tttccgtttt cagacacacg
     1861 gcttgccgaa agatcaatgc tcagcatgga atctgcagtt ccaagagctt cagtacacca
     1921 tagaattcct tcctccagat gttttttctc atcaagagtc agcctgtccc ttccgaaaat
     1981 ggtatttccc acagtatcaa tgtatctgtc catttcgaga agaactgaga gaagaatatc
     2041 ggcagtcttt ccggcataaa aatccagtct ttcggcagaa cccagacgga tggaactgag
     2101 ttctttttcc gtcttttcat tcccgtctgc cagacattta agaatgtatt ttccctgaga
     2161 ctgaagccac tcgtctatct gtctgtacac ctgacccaga ttctcctcat tctcaatctt
     2221 cgagtccagg agttcatcat taatatatat tttcattatc tgttttcatc cacgctgtaa
     2281 ctgtttgcct tcatctgctt aatgctgttg ataagatgat ttcccactgc cttgtttttt
     2341 cccactccct gctcca
//
LOCUS       DAOQGJ010000466         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29449_length_2355_cov_3.785217, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000466 DAOQGJ010000000
VERSION     DAOQGJ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29449_length_2355_cov_3.785217"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1586
                     /locus_tag="PK683_08340"
     CDS             <1..1586
                     /locus_tag="PK683_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08497.1"
                     /translation="DITEKKEAELNLLSAKQAAESANRAKSEFLANMTHEIRTPLNGI
                     IGFTDLLMQTEMNETQKKYLSAVYQSGSFLLEIVNDILDFSKIEAGKLELSVEKTDIY
                     EMCGQIQDIVRFQADKKKIGLSVIIPDDAPRFIYADEIRFRQVLINLLSNAIKFTVKG
                     RVELKLEILDRISSDETRFRFSVSDTGIGIAPANRQKIFEAFSQEDASTTKKFGGTGL
                     GLTISSRLLSLMNSSLQLESEQGKGSVFFFDIVLPTEAGLPDFPVYGQEFKSVLISGN
                     REYSILMIRDMLYMQNINSEAVSGSGEVFRLLSGENKFDALIIDSELYEADGLSSILK
                     IRKEMNIPFSDLPILVVCANEPDEKLRHRLQRERLAEFLLKPFHFHDLLDAMFRLKNQ
                     SSAIEETNTEQFTALIVEDNRTNMLLLSRLLSSLFPNVRILEAGTGNDALGILNSDLP
                     DIVFMDIQMPDKSGYETTEEIRRTHKWKDIPIIAVTAGSSSEERERCFQAGMNDFLLK
                     PIQKSGMESLIKQWLNRKP"
     gene            1796..>2355
                     /locus_tag="PK683_08345"
     CDS             1796..>2355
                     /locus_tag="PK683_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNH08498.1"
                     /translation="MAFDIDMIKNTYSRMKERVDAAKKVVGKPLTLTEKILYSHLWEQ
                     KDLKNFKRGADYVDFAPDRVAMQDATAQMALLQFMQAGRKKVAVPSTVHCDHLIQAKE
                     GAKADLDTANVTNKEVYDFLGSVSNKYGIGFWKPGAGIIHQVILENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADAMDVMAG"
BASE COUNT          703 a          470 c          561 g          621 t
ORIGIN      
        1 aggacattac tgaaaaaaaa gaagccgaac tgaatctgct ttcagcgaaa caggccgcag
       61 aatccgcgaa ccgcgccaag tcagaattcc tggcgaatat gacccatgaa atcagaactc
      121 ccttaaacgg gattatcggg tttaccgatc ttctgatgca gacggaaatg aacgagacac
      181 agaaaaagta tctttctgca gtatatcagt ccggcagctt tctgctggaa atagtgaatg
      241 acatattaga tttttctaaa attgaagcag gaaagctgga actctccgtc gagaagactg
      301 atatttacga gatgtgcgga cagattcagg atattgtcag atttcaggca gacaaaaaga
      361 agatagggct ttcagtcatt atccctgatg atgctccccg atttatttat gctgatgaga
      421 tcaggttcag acaggttctg atcaatcttc tgagcaatgc gattaagttt acagtgaaag
      481 gaagagtaga actgaaactg gagattctgg acagaatttc ctctgatgag actcgattcc
      541 ggttttcagt ttcggacact ggaataggca ttgcccctgc aaacagacag aaaatctttg
      601 aggcattttc gcaggaggat gcttccacca caaaaaaatt cggaggaact ggtcttggtc
      661 ttacgatctc aagcagactt ctttctctga tgaacagcag tcttcagctg gagtctgagc
      721 aggggaaagg atctgtattt ttctttgata tcgttcttcc tacagaagca ggacttcctg
      781 attttccagt ttacggtcag gaatttaaat cggttctgat ctccggaaac agggaataca
      841 gcatccttat gattcgggat atgctgtata tgcaaaatat caattctgag gcagtatccg
      901 gatcaggaga agttttcaga cttctctcgg gtgaaaacaa attcgatgcg cttattattg
      961 acagcgaact ttacgaagcg gatgggttaa gttcaatact gaagatcaga aaagagatga
     1021 atattccgtt ttctgatctt ccgatccttg tcgtctgcgc caatgaacct gatgaaaaac
     1081 tcagacacag acttcagaga gagagacttg cagagtttct tctgaaaccg ttccatttcc
     1141 atgaccttct tgatgcaatg ttcagactga agaatcagag cagtgcaatt gaagaaacaa
     1201 atactgaaca gtttactgcc ctgatcgtgg aagacaacag gacgaatatg cttcttcttt
     1261 ccagacttct gtccagtctc tttccgaatg tcagaatcct tgaagcaggt accggcaatg
     1321 atgctctcgg catcctgaat tcagatttgc cggatattgt atttatggat attcagatgc
     1381 ctgataaaag cggatatgaa acaacggaag aaatcagaag aacacataag tggaaagaca
     1441 ttccgatcat agcagttact gcggggagca gttccgaaga aagagaaaga tgctttcagg
     1501 ccgggatgaa tgattttctt ttaaagccta ttcagaagtc aggaatggag tctctgataa
     1561 aacaatggct gaacaggaag ccctgatctc attcaatgtc tggaccaaaa tgactctgtt
     1621 cggacaggtt catgatttct cataaggttt tccgtcctca atttgccttt caatcagttt
     1681 ttgaaaatgc aggagtcagt tttcgccgac aatcaaactg gcatctcttt ttttctttac
     1741 cttaaacccg aaatcaagga taccggttat ttgtgaaaac cctgtgggag gaagcatggc
     1801 atttgatata gatatgataa aaaacactta ttccaggatg aaggaacgag tggacgcggc
     1861 caaaaaggtc gtaggcaaac cattgacact gacagaaaag atactgtatt cccatctttg
     1921 ggaacagaaa gacctgaaaa acttcaaacg cggagccgat tatgtggatt ttgctcctga
     1981 cagagtggct atgcaggatg caacggcgca gatggctctt ctgcagttta tgcaggcagg
     2041 aagaaagaag gtagcagttc cttctacagt gcattgtgat cacctgattc aggcaaaaga
     2101 aggtgcgaag gccgatctgg atactgcaaa tgtcacaaac aaggaagtgt atgatttcct
     2161 tggttcagtt tccaataagt acggaatcgg attctggaag ccgggtgcag ggattatcca
     2221 tcaggtcatt ctggaaaact atgcattccc gggcgggatg atgatcggta cagactcgca
     2281 cactgtcaac gctggcggac tcggtatgat tgctatcggc gtcggcggtg cggatgcaat
     2341 ggacgttatg gcggg
//
LOCUS       DAOQGJ010000467         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29485_length_2354_cov_2.684211, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000467 DAOQGJ010000000
VERSION     DAOQGJ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29485_length_2354_cov_2.684211"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1359
                     /locus_tag="PK683_08350"
     CDS             217..1359
                     /locus_tag="PK683_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016755297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08499.1"
                     /translation="MENSKNDYQNPTRSEQIRYFGILAAAAAVIIISYFMGIPHYYLG
                     WMAFAVAAYSVAGNDAIQTVGTFIESKKHIHWIIKTAVFAGLLIIVHLYGWMTEDRQI
                     HFHRLDSFQEAREFNLLQLLSPIILVIITRLKAPVSTTFLILGLFGSSNVEKMLTKSF
                     LGYGIAFFSALLLWGILGYFQRNEYKEDYIADAKQEKFWGFMQWVSSMFLWVAWLLQD
                     TANIAVFISRKMSWGEFAAAMLILTAALAMIIKSDGGTIQEVVSEKTDVSTAKAATLI
                     DFIYAIVLFIFQYMSRLPMSTTWVFIGLLAGREFILHAVTKQDRPYLNTLRTIGKDVL
                     LASLGIAVSLFIIYLSAFLYSPSKTGWKELLPFQKIVKSSSMQKIQ"
     gene            1369..2325
                     /gene="ppk2"
                     /locus_tag="PK683_08355"
     CDS             1369..2325
                     /gene="ppk2"
                     /locus_tag="PK683_08355"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNH08500.1"
                     /translation="MGKHKKKLRKFSEQYQAVYNSILDDLEEELELPEEDENLDKNGR
                     AYEKLLSSAEESNDKISYLKELLRLQIQLIKMQDWITHTGYKLVVLFEGRDAAGKGGI
                     IKRITQRLNPRVCKVVALSAPSERERTQWYFQRYVSHLPGAGEIVLFDRSWYNRAGVE
                     RVMNFCTEEEYWDFLNTVPDFEKMICRSGIHLIKYWFSISDKEQEFRFKCRMNDPLKQ
                     WKLSPMDLESRIRWEDYTKAKEIMLEKTHIPEAAWNIVPADNKKAARLNCIADLLSKI
                     PYEEVQMKQVKLPKRKYNEGYRRNPSPEHLIVPQKYTGENLI"
BASE COUNT          744 a          428 c          510 g          672 t
ORIGIN      
        1 gaagttacaa tttattgaac aggaaattag aaattggtta caggattgtt tcttggcttt
       61 gcttaaaact gtccttttaa actttataac gtattttggc ggtcagaatt tatcaagatt
      121 ttttttgaaa ttcgggaatc tttaaaaagt catccttacg taataattcg gcagcagaat
      181 catataatta tacagtgtga aaatataaag gtatttatgg aaaattcaaa aaatgactat
      241 cagaatccga ctagatcgga acagatcaga tatttcggca tcctcgctgc ggctgctgct
      301 gtcatcatta tatcttattt tatgggcatt ccccattatt atctcggctg gatggcattt
      361 gctgtggctg catattcagt agcgggcaat gatgcgattc aaactgtagg aacattcatt
      421 gaaagcaaga aacacataca ctggattata aaaactgctg tctttgcagg acttctgatc
      481 attgtgcatc tttatggatg gatgacagaa gaccgacaga ttcatttcca caggctggat
      541 tcattccagg aggcaagaga attcaatctt cttcagctgc tttcgccgat tatccttgta
      601 attattacca gactgaaagc tcccgtttct actacttttc tgatcctggg gctgtttgga
      661 agcagcaacg tagagaaaat gctgacgaaa tcttttttgg gatacggaat tgcttttttc
      721 tccgcccttc ttctttgggg aattttaggt tattttcaaa gaaatgaata taaggaagat
      781 tacattgctg atgcaaaaca ggaaaaattc tggggattta tgcagtgggt ttcttcgatg
      841 tttctctggg ttgcatggct tcttcaggac actgcaaaca tagctgtttt tatttccaga
      901 aaaatgagtt ggggagaatt tgctgccgct atgctgattc tgacagcagc tcttgcgatg
      961 atcattaaat cggacggggg cactattcag gaagttgtaa gtgaaaaaac agatgtcagc
     1021 actgcaaaag cagctacatt gatagatttc atctatgcaa ttgtgctttt tatatttcag
     1081 tatatgagca gactgcctat gtcgacgacc tgggtgttta tcggacttct tgcaggacgg
     1141 gaatttattc ttcatgctgt gacaaaacag gacagacctt atctgaatac actcaggaca
     1201 atcggcaaag atgttctcct ggcttctctc ggaatagcag taagtctgtt tattatatat
     1261 ttatccgctt ttttgtattc tccctcaaaa acaggctgga aagaattgct tccttttcag
     1321 aaaatagtaa aaagttcatc tatgcagaaa attcagtgag gattcattat gggaaaacat
     1381 aaaaaaaagc ttagaaaatt cagtgagcag taccaggcag tctacaattc catccttgac
     1441 gacctggaag aagaactgga acttcccgaa gaagacgaaa atctggacaa aaacggaaga
     1501 gcatatgaaa aactgctcag ctctgcagaa gaaagtaatg ataaaatatc ttatctgaag
     1561 gaacttctca gacttcagat tcagctcatt aaaatgcagg actggattac ccatacaggg
     1621 tataagctgg tggttctgtt tgaaggaaga gatgccgcag gaaaaggcgg gatcattaaa
     1681 agaatcactc aaagactgaa cccgagggtc tgcaaggtag tggcactgag tgcaccttcg
     1741 gaaagagaga gaactcaatg gtattttcag agatatgtat cacatcttcc gggagcaggt
     1801 gaaattgttc tgtttgacag aagctggtac aaccgggccg gggtggaaag agtcatgaat
     1861 ttctgcactg aggaagagta ctgggacttc ctgaatactg ttcctgattt tgaaaagatg
     1921 atctgcagat caggaatcca tctgatcaaa tactggtttt ccatctctga caaagaacag
     1981 gaattcagat ttaaatgcag gatgaatgat cctttgaagc agtggaaact cagcccgatg
     2041 gatctggaat ccagaatccg atgggaagac tacacaaagg caaaggagat tatgctggag
     2101 aaaactcata tacctgaagc tgcctggaac attgtaccgg cagacaataa gaaagccgca
     2161 agacttaact gcattgccga tcttctttct aagattcctt atgaagaagt tcaaatgaag
     2221 caggtcaaac ttcctaaaag aaaatataat gaaggttaca gacgaaatcc ttcgcccgaa
     2281 cacctgattg ttcctcagaa atataccggt gaaaacttaa tctaagaaat ccaaagtgta
     2341 aaatgagtta acca
//
LOCUS       DAOQGJ010000468         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29555_length_2351_cov_3.091899, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000468 DAOQGJ010000000
VERSION     DAOQGJ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29555_length_2351_cov_3.091899"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PK683_08360"
     CDS             complement(<1..425)
                     /locus_tag="PK683_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH08501.1"
                     /translation="MRYKLLGRSGLRVSELCLGTMTFGEEWGWGAGKEESFQMYKKFI
                     DAGGNFIDTANRYTEGTSEKFLGEFIGSERPNIVLATKYTLFEDKRYLNRSGNHRKNM
                     VQSLENSLKRLKTDYIDLYYLHVWDSTTPEDEILRALDD"
     gene            656..1714
                     /gene="rlmN"
                     /locus_tag="PK683_08365"
     CDS             656..1714
                     /gene="rlmN"
                     /locus_tag="PK683_08365"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNH08502.1"
                     /translation="MNTRSEPGKKLKTVINGKSVRELEEFVLSLGEKKFRAVQIYEGL
                     YSKRWKSFSEFTNLSGELRRKLEETAEIKSIHLVSKQVSSDGTVKGLFEAEPGKNIEA
                     VWIPNSSDGRKTICISSQSGCTLSCEFCATGRLDFQGNLKAWQILDQIVQMEEIAGDR
                     CTNVVFMGMGEPMHNYANVMTAAHILHDAKAFGLGAKRITISTAGVIPSIRKFIFEKQ
                     PFNFAISLNHPDPEKRKDIMGIDQRFPLKELLLTAREFTKTLGRKITFEYIMIPDVNM
                     GEKNARLLVKLARSVNCKINLIPLNTEFNGWRRPSEEEMEEFRNMILPSNVPILNRRS
                     PGKDIDGACGMLSLKTKN"
     gene            complement(1704..>2351)
                     /locus_tag="PK683_08370"
     CDS             complement(1704..>2351)
                     /locus_tag="PK683_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08503.1"
                     /translation="DLRNYKAEYEGEKFREYQLPPKRDKPALINPKDVRRPFDPNMII
                     PPPAGLRRPAQAQNFNFANSPVNPLTGELNPSAILGNQNGGRQKREEQKKQVEEASKK
                     AYTETPYRRGEIIFFTTLPFASALAIGAALLVDAATGANKRNKITGKRNFSFYRSAGG
                     AAFVGLTAVGLSLGNVYLDRLQVEEFEKQKKINPELDRHLGYDFRMDFTLGQINF"
BASE COUNT          640 a          491 c          525 g          695 t
ORIGIN      
        1 atatcgtcaa gtgctctcag aatttcatcc tcaggagtcg tcgaatccca gacatgcaga
       61 taatataaat ctatataatc agttttcagt cttttcaggc tgttttcgag agactgaacc
      121 atattttttc tgtggttccc ggacctgttc agatatcttt tgtcttcaaa aagagtgtat
      181 tttgtcgcaa gcacaatatt cggtctctcg ctgccgatga actcgcccag gaatttttcg
      241 cttgtgcctt ccgtgtaccg gtttgctgta tcaataaaat tgcctcccgc atcgatgaat
      301 tttttataca tctgaaagct ttcttccttt cctgctcccc atccccattc ttctccgaat
      361 gtcattgttc ccagacagag ttcggaaacg cggagtccgc ttcttcccaa gagtttatat
      421 cgcatacagt tctcctatct gttccatttt aaaaaaactg tcctgcgaaa accatcagtt
      481 ttttcaggca gactgctttc cggaaggacg ctgtcaaatg attttataat tatgccagag
      541 aggctgtcgg cagattctgc ataaagtgtt ttatttcggc tgaacggttc agaacttaac
      601 tgatgcgact tgacaaagtc gcactaaaaa gaaacttgca gtctggaaat gagttttgaa
      661 cactcgttct gagccgggaa aaaaattgaa gacagtcata aacggaaaat cagtgaggga
      721 actcgaggaa tttgttcttt ccttgggaga gaaaaagttc agagcagtgc agatctatga
      781 aggactgtat tctaaacgct ggaaatcctt ttcagaattc acaaatctgt cgggtgaact
      841 cagaagaaaa ctcgaagaga ctgcagagat taaaagcatt catcttgtat caaagcaggt
      901 ttcctctgac ggaacagtca agggactttt tgaagctgag cctggtaaaa atattgaagc
      961 ggtctggata cccaattcgt cagacggaag aaagacaatc tgcatttcgt ctcagtcggg
     1021 atgcactctt tcctgtgaat tctgtgctac aggacgcctc gattttcagg gcaatctcaa
     1081 ggcctggcag atcctggatc agattgttca gatggaagag attgcgggag acaggtgtac
     1141 taatgtcgta tttatgggaa tgggggagcc gatgcacaat tatgcaaatg taatgactgc
     1201 agcccatatt cttcatgatg caaaagcatt cgggctcgga gcgaaaagaa tcacgatttc
     1261 tacagcagga gtcataccga gcattcgtaa atttattttc gagaaacagc ccttcaattt
     1321 tgcgatttca ctgaatcatc ctgatccgga aaaaagaaag gatattatgg ggattgatca
     1381 gaggtttcca ttgaaggagc ttcttttgac tgcaagagag tttacaaaga ctctgggaag
     1441 aaagatcaca tttgaataca tcatgatacc cgatgtaaat atgggtgaaa aaaatgcccg
     1501 actgcttgta aagcttgcca gatcagtgaa ctgcaaaatc aatctgattc cgctcaatac
     1561 tgagtttaac ggatggagaa gaccgagtga ggaggaaatg gaggaattca gaaatatgat
     1621 tctaccgtcg aatgttccta tattgaacag gagatccccc ggcaaggaca ttgacggagc
     1681 ctgcggaatg ctttcactga aaactaaaaa ttgatctgtc cgagagtgaa gtccattctg
     1741 aaatcatatc ccagatgtct gtccagttca ggattgattt ttttctgttt ttcaaattct
     1801 tccacctgaa gtctgtccag atatacattt cccaatgata gaccgactgc agtgagtcct
     1861 acaaatgcgg ctcctcctgc agatctgtaa aaactgaaat ttcttttgcc tgtgatcttg
     1921 tttcttttgt tggccccggt tgcggcatcc acaagaagcg cagccccgat tgcaagtgca
     1981 gaagcaaagg gaagtgtcgt aaaaaagatg atttctcctc ttctgtacgg agtttctgta
     2041 taggcttttt tggaagcttc ttcaacctgt tttttctgct cctctctttt ctgtcttccc
     2101 ccgttctgat ttcctaaaat cgcggaagga ttgagttctc cggtaagcgg gttgacagga
     2161 gagtttgcaa agttgaaatt ctgtgcctgt gcaggtcttc tgagcccggc aggaggggga
     2221 atgatcatat tcggatcaaa tggacgtctt acatcctttg gattgatgag tgcgggcttg
     2281 tctcttttgg gaggaagctg gtattccctg aatttttccc cttcatactc tgccttgtaa
     2341 tttctcagat c
//
LOCUS       DAOQGJ010000469         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29583_length_2350_cov_2.654031, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000469 DAOQGJ010000000
VERSION     DAOQGJ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29583_length_2350_cov_2.654031"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK683_08375"
     CDS             complement(<1..534)
                     /locus_tag="PK683_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08504.1"
                     /translation="MKYSKMISIFGILSIAALMAGCEGKPKKAKILTTTTSILVDEKS
                     GTTASGASVSTSVSSGSYTVTLPTGLSLSGTPGGAFTGITYQGSPLEVSGFVGQIPDY
                     GQTSSSSLGSSLLALLNGQTASTNLNETSIISTNAVAQGTGNTTIYHITVTTDSSTNV
                     TSLSNLILGIIGVNAAGG"
     gene            701..1948
                     /locus_tag="PK683_08380"
     CDS             701..1948
                     /locus_tag="PK683_08380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH08505.1"
                     /translation="MKNGKFHKISIITPVLNAAEFLERTIQNVISQNYPELEYIIVDG
                     GSTDGTLEIIKKYPSVKLIQEKGSQSKAFNIGFLAATGDILGAVNGDDTYCEGIFHFV
                     NEYFLNQPDCEFLMGNVQFIENGKPGFLNVPRHTMPEMMYWWEKNAYCFNPVGYFYTC
                     KVRDSVPPFKENNHYDMDHSFLLEAGSRFRIHKVEKTFGHFYFHEKNKTSQLSRENFF
                     THYLNVKRQSVEDNLYLLSEQDKLQFKLCETFFLCRLFVKEVIYGKFRFQMSEYLQSV
                     PYFLKFFFSGLWIIFLHYFVLKKSQNRILSFSVSCISLFFGSVKKSPLFQEKIFRIYR
                     EDYSKVSGSFRLNASGTIDGSEKFSSWSVSEGKLRLTDEDGNLIYLFFLSFGSTESFV
                     LAGSRPDEIKMYILSEYKAGLTH"
     gene            1945..>2350
                     /locus_tag="PK683_08385"
     CDS             1945..>2350
                     /locus_tag="PK683_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08506.1"
                     /translation="MKTETVKDGRTVLQNILLQDDTERRDLYFKSRGDLILADKGRVI
                     LKPESFLSFHSFFNALSIQHYADIFSIKGLFLNFKYAGEGKAVIKGVNEAGIEETISE
                     FSLAFSNSRKPVSFEIMNAEKHKYIFPVFLSES"
BASE COUNT          687 a          422 c          485 g          756 t
ORIGIN      
        1 cccgcctgct gcattgactc caataattcc aagaatcagg tttgacaaag acgtaacatt
       61 tgtgctgctg tcagtagtaa ctgtaatatg ataaatagtc gtatttccgg ttccttgcgc
      121 tactgcattt gtggaaatga ttgaagtttc attcagatta gttgatgctg tctgaccgtt
      181 aagaagagcc agcagagagc ttcccagtga actggaactt gtctgaccgt aatcaggaat
      241 ctgtcctaca aaacccgaga cttccaaagg acttccctga tatgtaatcc ctgtaaatgc
      301 tccgccggga gttccgctca ggcttaaacc tgtagggagt gtgacagtat aagaaccgga
      361 tgatacggaa gtggatacag atgctccgga agccgttgtt ccgctttttt catccactaa
      421 aatacttgtt gtagttgtaa gtattttggc ttttttgggt ttgccctcac atcctgccat
      481 gagcgcagcg atggagagaa taccgaatat tgaaatcatt tttgagtatt tcatatttaa
      541 tctcctcttt cagttttatt atacgttttt ttcagtgaaa ttatttccct gatctcgttt
      601 ttttcccaaa aaactgaaaa tctgcttgaa aaatcaactt ttgatgaaag tccttgcctg
      661 tcagaagccg tgcagaaatc tggaatggac ggtttttccc atgaaaaatg gaaaatttca
      721 taaaatttcg attattaccc ctgttctcaa tgcagctgaa tttcttgaaa ggaccattca
      781 aaatgtaatt tctcagaact atcccgaact tgagtacatt attgtggacg ggggatcgac
      841 ggacggtacg ctcgagatca ttaaaaaata tccttctgta aaacttattc aggagaaggg
      901 gagtcagtca aaggccttca atattggatt tcttgcagcg acaggcgata ttctcggggc
      961 tgtcaatgga gatgatactt actgtgaggg aatatttcat tttgtaaatg aatattttct
     1021 gaaccaaccg gactgtgagt ttctgatggg aaatgtgcag tttatcgaaa acggaaagcc
     1081 aggatttctg aatgttcccc ggcacacaat gcctgaaatg atgtactggt gggaaaaaaa
     1141 tgcatactgc ttcaatcctg taggatattt ttatacctgc aaagtgagag attctgttcc
     1201 gccctttaag gaaaacaatc attatgatat ggatcattct tttctgcttg aggccggaag
     1261 cagattccga atacacaagg tggaaaagac attcggccat ttttactttc acgagaaaaa
     1321 taaaacctcc caattgagca gggagaattt ttttactcat tatctgaatg tgaaaaggca
     1381 gtctgtcgaa gataatcttt atcttctttc cgaacaggat aaacttcagt tcaaactgtg
     1441 tgaaactttt tttctttgca gactttttgt gaaagaggtt atttatggaa aattccggtt
     1501 tcagatgagt gaatacctgc agtcagttcc ttacttttta aagttttttt tctccggact
     1561 ttggattata tttctgcatt attttgtgct gaaaaaatct caaaacagaa ttctttcatt
     1621 ttctgtttcc tgcatttccc tttttttcgg atcagtaaaa aaaagtcccc tttttcagga
     1681 aaagattttc agaatttaca gagaagatta ttctaaagtt tccggttctt tcagacttaa
     1741 tgcaagtgga acaattgatg gaagtgaaaa gttcagcagc tggtctgttt cggaagggaa
     1801 acttagactg accgatgagg atggaaatct tatttatctt ttctttcttt ctttcggcag
     1861 tactgaaagt tttgttttgg ccggaagcag accggatgaa atcaaaatgt atatattaag
     1921 tgaatacaag gcggggttga cacattgaaa acggagacag taaaagacgg ccgtacagtt
     1981 cttcagaata tccttctgca ggacgatacc gaaaggcggg acctttattt taaaagcagg
     2041 ggagatttaa tccttgcaga taaaggcaga gtgattctga agccggaaag ttttctgtcc
     2101 tttcattcct tttttaatgc cttgagcatt cagcattatg cagatatatt ttcgataaaa
     2161 ggtttattct tgaattttaa atatgcaggt gaaggcaagg cagtaataaa aggcgtaaat
     2221 gaagcaggta ttgaagaaac gatttctgaa ttcagtcttg ctttttcaaa ttctcgcaaa
     2281 ccggtatctt tcgaaattat gaacgctgaa aagcataaat acatttttcc tgtctttctt
     2341 tcggaaagca
//
LOCUS       DAOQGJ010000470         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29606_length_2348_cov_3.480593, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000470 DAOQGJ010000000
VERSION     DAOQGJ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29606_length_2348_cov_3.480593"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PK683_08390"
     CDS             <1..337
                     /locus_tag="PK683_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH08507.1"
                     /translation="IGSFISLFLLGESLNIGSVLGFLLLSGTAVDGAVLYYEEIQRTE
                     GDRLRCAADVFRSVFINLLTTLCGLSPVILNILPSSEWNRSLACAVFGGLISSAGLTL
                     ILLPVYFKK"
     gene            337..>2348
                     /locus_tag="PK683_08395"
     CDS             337..>2348
                     /locus_tag="PK683_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH08508.1"
                     /translation="MIFRYIVFSRRRRIFLFAFFSSLFLLSAIRFPHSRITAYFREPP
                     KVLLIQTEYPGAHPEKTEKKITEPLEESLYGTSGISELESYSETGRSEIRIFFHENAD
                     IRKSELEIRERVENAFRRFPSDVRYPSIENFRRENSPDMILKVEKSGYEEERQFAETV
                     LKPEILKEKSVSGISIYGGAGWEFLISVSPEKLRKYSLSAEEILGAVRKSRVQSRITG
                     VQYNDKVYNISIKSAADTEDLISGIPVLNSKGRTVFLKDVSEVHYSKTRPTSVFRRNG
                     REETAVYIHFADHSGASQVRKILSQEKFQGRTALIYDRSEAEKKEWFSVFSTVSLLCT
                     VIFFLKMRKRRTVFTGISGGLLTFFILISFSEKFTIPDFYSVFIGIFIVSIGIRWNTA
                     AVFMLLLSGAGFVLSGFLENFSLQKLQIVLFLKLLFSGLLYLGLSVYIRILCRSFGLE
                     RKISRMQRFNKVFHFIISYTLFSSALLADSVWKFLLKYRSVIPSPVCIAGAAASAFLS
                     VYAEYHYLQTKISFAFAEKTSAVTANLELPSGFSLSASERAAGVLEGRLLRLPELETY
                     SLSENDHSSVTVRTEKSINSKKNWIHQLKETAGKTEPAYLYFQTEEGASPEEELTFDV
                     YGSDFIKMNDFISESLRFSSSLNGVLESVYRYKPAREEILFRLDRE"
BASE COUNT          656 a          462 c          509 g          721 t
ORIGIN      
        1 cataggaagt tttatttcgc tttttcttct tggagaatct ctcaacatcg gatctgttct
       61 gggatttctg cttctttccg ggacagccgt agacggagct gtcctttatt atgaggaaat
      121 tcagagaacc gaaggggaca gactgcgctg tgcggccgat gtgttccgat ctgtttttat
      181 caatctgctc actacccttt gcgggctttc acctgtcata ctgaatattc ttccctcctc
      241 tgaatggaac cgctcccttg cctgcgctgt attcggagga ctgatctctt ctgcaggact
      301 gacactgatc ctccttcctg tgtactttaa aaaataatga tattcagata tatcgtattc
      361 agcagaagaa ggaggatctt tctttttgcc tttttttctt ctctgtttct gctttctgca
      421 atccgttttc ctcattccag aattactgct tattttcgcg aacctcccaa ggtgcttctt
      481 attcagacag aatatcctgg agcacatccc gaaaaaacag aaaagaaaat cactgaacct
      541 ttggaagaat ctctttatgg aacctcagga atatcagaac tggaatcata ttcagagaca
      601 ggcaggtctg aaatcagaat attttttcat gaaaatgctg atattcgaaa atcagagctc
      661 gaaatccgcg aaagagtgga aaatgcattc agaagatttc cctcggatgt aaggtatcct
      721 tccattgaaa acttcagaag ggaaaactct ccggatatga ttctgaaagt ggaaaaatca
      781 ggatacgaag aggaaagaca gtttgctgaa actgtgctta agccggaaat tctcaaggag
      841 aaatccgttt caggaatcag catatacggc ggagcgggct gggaatttct gatttctgtc
      901 tctccggaaa aactcaggaa atactctctg tctgctgaag agattctcgg tgcagttaga
      961 aaaagcaggg ttcagagcag gataactgga gttcaatata atgataaagt ttataatata
     1021 tcaataaaat ccgctgcaga tactgaggat ctgatttcag gaattccagt tttgaattca
     1081 aaaggcagga cagtttttct aaaggacgta tcagaagtac actattcaaa gacaagaccg
     1141 acgtctgttt tcagaagaaa cggcagagaa gaaacagctg tatacattca ttttgcagat
     1201 cattccgggg cttctcaggt cagaaaaatt ctgtctcagg aaaaatttca ggggagaaca
     1261 gctctgattt atgatcggtc ggaagcggaa aaaaaggaat ggttttcagt attcagcact
     1321 gtgtctttgc tgtgcacagt gatcttcttt cttaaaatga gaaaaaggag gacagttttt
     1381 accggaattt ccgggggcct gctgacattt ttcatcctaa tttcattttc tgaaaaattt
     1441 acaattccgg atttctattc cgtttttata gggattttta tagtttcgat cgggatcaga
     1501 tggaatacag ctgccgtatt catgctgctt ctttccggag caggatttgt tctgtcgggc
     1561 tttttggaaa atttttcact tcagaagctt cagattgttc tctttctgaa gcttcttttc
     1621 agcggccttc tctatctggg actctcagtt tacattcgaa ttctctgcag aagtttcgga
     1681 ctggaaagga aaatttcaag gatgcaaaga ttcaataaag tattccattt tataatttca
     1741 tatactttgt tttcttctgc tctcctggcg gattctgtat ggaaatttct tctgaaatac
     1801 agatctgtga ttccttcacc tgtatgcatt gcaggagctg cggcgagtgc atttttatcc
     1861 gtttatgcag agtatcatta tcttcagacg aagatcagtt ttgcctttgc ggaaaaaaca
     1921 tccgctgtta cggcaaatct ggaactgcct tccggttttt cactttcagc ttcagaacgt
     1981 gcggcgggag tattggaagg aaggcttctc aggcttcctg aactcgaaac gtattctctc
     2041 tcggagaatg atcattcttc agttactgta cggactgaaa aaagcataaa ttccaaaaag
     2101 aactggatac atcagctgaa agagactgca gggaaaacgg aaccagccta tctgtatttt
     2161 cagactgagg agggagcatc tcccgaagag gaactgacat ttgatgttta tggatcagat
     2221 ttcattaaaa tgaatgattt tatatccgag tctctccggt tttcgagttc tctgaacgga
     2281 gtgctggaat cagtttacag atacaagccg gcgagagagg aaattctttt tcggctggac
     2341 agagaaaa
//
LOCUS       DAOQGJ010000471         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29667_length_2345_cov_3.024017, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000471 DAOQGJ010000000
VERSION     DAOQGJ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29667_length_2345_cov_3.024017"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PK683_08400"
     CDS             <1..414
                     /locus_tag="PK683_08400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNH08509.1"
                     /translation="ELNCPVVIHSRDAAEDTYSILKEYSGKARGVIHCFTYDTEYALK
                     FFNLGYFISFSGILTFNSAKDIQKAASAVPLEGILIETDAPFLAPTPHRGRRNEPAYM
                     PQILEKLFSLRDESNIEVEEKIYENSLKFIERKGI"
     gene            418..1671
                     /gene="serS"
                     /locus_tag="PK683_08405"
     CDS             418..1671
                     /gene="serS"
                     /locus_tag="PK683_08405"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNH08510.1"
                     /translation="MLDLNMVTANPEILKEMLQKRGNEDSSHLEELEETLKNRKKLQQ
                     DAETLRSERNRVSKEIGIRKGKGEDISEISHSMKEVGNRIKEIETRLEELEEKIQTIN
                     LHFPNLLDPEVPFGKSDADNVETHQFGTKRTFSFTPKPHFEIGENLKILDFERGVKLA
                     GSRAYTYFGKAAKLERALMNFMLDTHSREHGYEEVWVPMLVNDDSMMTTGQYPKFKDE
                     YYRLEKDHLNLIPTAEVPLTNLFRDEILREEELPKSIMAHTSCFRREAGSYGRDTKGL
                     VRVHQFQKVELVKFCRPEDSEKEHRLMLLNAENILKKLNLHYRVLLLCSKDTSNSSSK
                     TFDIEVWMPGLGRYMEISSVSNFKDFQARRGKIRYKSKEGRNLLVHTINGSGLAIGRT
                     LAAVIENYQTESGDFEIPEVLTKYF"
     gene            1717..2103
                     /locus_tag="PK683_08410"
     CDS             1717..2103
                     /locus_tag="PK683_08410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH08511.1"
                     /translation="MSEILELENECFPSGFWNRSQILSHLNSFSALGIYSDNLLSAYV
                     LFSSAAEETEIFRIGTRVRFRRKGLSGKLIQELEKISASILLEVSSRNIEAQRLYEKS
                     GFRLSGRRKKYYSDDSDALIFKKIRA"
BASE COUNT          793 a          479 c          509 g          564 t
ORIGIN      
        1 gaactgaact gccctgtcgt cattcattcg agagacgctg ctgaggacac ttattccata
       61 ctgaaagagt attccggaaa ggcaagagga gtgattcact gcttcactta tgatactgag
      121 tatgcattaa aattctttaa tttgggatat tttatatcct tctccggaat actcacattc
      181 aacagtgcga aggatattca gaaggctgcc tcagcagttc ctctggaagg aattctcatc
      241 gaaacagatg ctccctttct tgccccgact cctcacagag gcagaagaaa cgagccggct
      301 tatatgccgc agatacttga aaaactcttc tcacttcggg atgaatcaaa tatcgaagta
      361 gaagaaaaaa tatatgaaaa ttcattaaaa tttatagaaa gaaaaggaat ctaagcaatg
      421 ctagatctca atatggtgac agccaatcct gaaatactga aagaaatgct ccagaaaaga
      481 gggaatgagg attcttccca tctcgaagaa ctcgaagaga cactgaaaaa cagaaaaaaa
      541 ctccagcagg atgctgaaac actgcgctca gaaagaaaca gggtcagcaa ggaaatcgga
      601 atcagaaagg gaaagggaga ggacatttcc gaaatctccc attccatgaa ggaagtagga
      661 aacaggatca aggagatcga aacaaggctt gaagaactcg aagaaaaaat tcagacaatc
      721 aatcttcatt tcccgaatct tctcgacccg gaagttcctt tcggaaaatc agatgcggac
      781 aatgtagaaa cgcaccagtt tggaacaaaa agaaccttct cctttactcc taagccccat
      841 tttgaaatcg gcgaaaatct gaaaattctg gattttgaaa gaggcgtgaa gcttgcaggc
      901 tcgagagcat atacttattt cggaaaggct gcaaagcttg agagggcact gatgaatttt
      961 atgctggaca ctcattccag ggaacacgga tacgaggaag tatgggtgcc gatgcttgtc
     1021 aacgacgaca gtatgatgac cacaggacag tatccgaaat tcaaggacga atactacaga
     1081 ctggaaaaag accatctgaa tctgattccc actgcagaag tgcctctgac aaatcttttc
     1141 cgagatgaaa tactcagaga ggaagagctt ccaaaaagca taatggctca tacatcctgt
     1201 ttcagacggg aagcaggatc ctacggaaga gatacgaaag gcctggtgag agttcatcag
     1261 tttcagaaag tggagctggt aaaattctgc aggccggagg attccgaaaa agagcacaga
     1321 cttatgctct tgaatgcaga aaatatcctt aaaaaattaa atcttcatta cagagtgctt
     1381 cttctctgtt caaaggatac atccaattcc tcatcgaaga ctttcgacat tgaagtctgg
     1441 atgcctggtc tcggaagata tatggaaatc tcttcagttt ccaatttcaa ggattttcag
     1501 gcaagaagag gaaagatcag atacaaatca aaagaaggaa gaaatctgct cgttcacacg
     1561 atcaacggtt caggacttgc catcggcaga actcttgctg ctgtgattga aaattaccag
     1621 acggaatcag gcgatttcga aatcccggaa gtcctcacaa aatatttcta aacattggct
     1681 gcgtgttttg aaatccggaa tctggggaca gagaatgtgt ccgaaatcct ggaactggaa
     1741 aatgaatgtt ttccgtccgg gttctggaac cggtcccaga ttctttcaca tttgaactcc
     1801 ttctctgctc tgggaatata ttcagacaac cttctttctg cctatgttct tttcagttca
     1861 gcggctgaag agacagaaat attccgaatt gggacacgtg tccgattcag aagaaaggga
     1921 ttgtccggaa aactgattca ggaactcgaa aaaatttctg cctccatact ccttgaagtg
     1981 agttccagaa acatagaagc ccagagacta tacgaaaaat cgggatttcg actttcagga
     2041 aggcggaaaa aatactactc tgacgattca gatgctctga tcttcaaaaa aatccgcgct
     2101 taactttttt taaatagatt gacaaaaaaa ccctgctaaa aaaaactttt ccctagaatt
     2161 agcactctaa ctgattgagt gctaaaaagg aactgccgga aaaaaaggct ggattcctga
     2221 gattttgaaa ccaaatgctc tccagacagc tggagctgac aaaaacgagg aagatatgga
     2281 actgagtcca agacacagac tgatactgaa agctctgatc gatgattttg ttgcagaaag
     2341 cagac
//
LOCUS       DAOQGJ010000472         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29683_length_2344_cov_3.943644, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000472 DAOQGJ010000000
VERSION     DAOQGJ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29683_length_2344_cov_3.943644"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..558
                     /locus_tag="PK683_08415"
     CDS             340..558
                     /locus_tag="PK683_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08512.1"
                     /translation="MINALRTYHEYFTVWILDKEPVNNSFFTTSTFAAQGVALVSVYD
                     KGQIIALETGLKFLKLTEFWSLAFLRSY"
     gene            complement(642..1523)
                     /locus_tag="PK683_08420"
     CDS             complement(642..1523)
                     /locus_tag="PK683_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08513.1"
                     /translation="MNNEIEVIKEQSGQMTPMQRMAELKERKLIIGSVMKEVMKENEH
                     FGKIPGTNKQTLLKSGAECLTSVFGLAPRFTVKQDDFGNGHREYVITCELYAANGNFL
                     GSGVGSCSTMEKKYRYRSIREYISELPKDFKDRKAYYYSLGQVGWKDENNIWGLYKQV
                     QGENPDIADQYNTVLKMAKKRALVDATLTVTGASDIFAQDVEDFVDHPEQTAKWEMNW
                     TNFFTEKWNSFPAEAKASEKMESIIRSYGNLPEWNDISKVLETIWTNLIKGKELDEKS
                     KKASDAMIDAIKAGKLQ"
     gene            complement(1652..1855)
                     /locus_tag="PK683_08425"
     CDS             complement(1652..1855)
                     /locus_tag="PK683_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08514.1"
                     /translation="MNSKTYWLAMSMGILSMAPNYNSYDFSRATSGHKKSFTDIQRRS
                     AQIVKKRNRRRNKAARQSKKANR"
BASE COUNT          568 a          519 c          405 g          852 t
ORIGIN      
        1 tccagccata attgaatttt acggctggat tttttgaggt caagctgaaa tattctcccg
       61 ttcggtgcgg gtcctgattg cgtctttgat cctgtatatt gtttcttcca ggacagcagg
      121 gttataatgt tcaggattcc aaagatgcag catatcctgt atgagagggt gttttatcag
      181 ttgataaatc tgatcccgta atggctgacc atttttatac cagactgcct gctttgcttt
      241 ttcttgtgca agtttcgcct gctttctttt ttctgcctga actgcagatt ttaactgctt
      301 ttcgtgattc cgaatatcat tctgaacagc aggaatgaag tgattaatgc actcagaacc
      361 taccacgaat atttcaccgt ctggattctt gacaaagaac ctgtaaacaa ttcctttttt
      421 accacaagca cattcgccgc ccagggcgtc gcattggtta gtgtatacga taagggacag
      481 atcattgcac ttgagaccgg tctgaagttt ttgaagttga ctgagttttg gagccttgct
      541 tttcttcgct cctattagat catgattatt tacaagtgtt cccattgatt tctcctatat
      601 aaagaaatcg gctggaatga ctccagcctt tagaagtttt tttactgcag tttacctgct
      661 tttattgcat caatcattgc atcggatgct tttttagatt tttcatcgag ttctttccct
      721 ttgataaggt tagtccaaat ggtttcaaga actttagaaa tatcattcca ttctggcaga
      781 tttccataag accgaataat gctttccatt ttttccgatg cctttgcttc tgcaggaaat
      841 gaattccatt tttcggtaaa gaagttagtc cagttcattt cccactttgc agtctgttca
      901 ggatgatcca cgaagtcttc aacgtcttga gcgaatatgt cactcgctcc tgtgactgtc
      961 aatgtagcat caaccaaagc tcgtttcttt gccatcttta gaacggtatt atactggtct
     1021 gcaatatccg gattttcacc ttgaacttgc ttatacagcc cccatatgtt gttttcatct
     1081 ttccatccaa cctggcctaa agaatagtaa taagccttac ggtctttgaa gtcctttgga
     1141 agttctgaaa tgtattctct gatgctccga taacggtatt ttttttccat tgtagaacat
     1201 gacccgacac ctgaaccaag aaaatttccg tttgctgcat acagttcgca tgtgatcaca
     1261 tattccctat gaccatttcc aaagtcatcc tgtttgactg tgaatcttgg agctaaccca
     1321 aaaacagaag tcagacattc tgcacctgat ttcagaaggg tttgcttatt agttcccgga
     1381 atcttgccaa aatgttcatt ttctttcatg acttctttca tgactgaacc tataatgagt
     1441 ttcctttctt tcagttctgc cattctttgc atgggggtca tttgacctga ctgctctttt
     1501 attacttcaa tttcattgtt catattctct ccttttcttg ttcttcattg aacagcttct
     1561 ccagtgcttc tgcaagatgc tcaaatcttt ttgcagggat accccccctg cgtagtttga
     1621 tactgttcaa taatttccag aattttttct ttcatcggtt cgcctttttt gactgtctgg
     1681 cagccttgtt tcttctgcgg tttcttttct tcacaatctg tgcagaacgt ctctgaatat
     1741 ctgtaaatga tttcttgtgt ccacttgtag cacgtgagaa atcatacgaa ttataatttg
     1801 gtgccattga cagaattccc atgctcattg caagccaata tgttttactg ttcatctcaa
     1861 ttctccttta tttcattatc ggctagactg ccgaattcct tgaatacgtt tcagcactaa
     1921 ttcaggggtg tgttccacaa tatgattccc atgaaatttg cactgccaca ttctgcctgc
     1981 agtcatagta atgtctgcaa taattttccc attctcaatt accgagtaaa aagttttgaa
     2041 ctccatcagt gtcatataaa ttcctttaat tgctttttcc aagcatccaa tatttcacaa
     2101 cgtgcatcaa attcaatatc tgatctgcaa tgccgaaaat ctttcataaa tcggataagg
     2161 cgtttttcct gatattccaa tgcgccttca atgccaaaat cgtttacgtt aatctgtgtc
     2221 agtgctgtca tctatttcct cgctttcatc aaagcctgat atttctctca ggcagtcagg
     2281 gcagatcaga gactgtagaa ttttttagtc aataactgcg attccttcaa tcgtcagttt
     2341 tttt
//
LOCUS       DAOQGJ010000473         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29684_length_2344_cov_3.751857, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000473 DAOQGJ010000000
VERSION     DAOQGJ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29684_length_2344_cov_3.751857"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1222)
                     /locus_tag="PK683_08430"
     CDS             complement(239..1222)
                     /locus_tag="PK683_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNH08515.1"
                     /translation="MEEDIRIHSLSSSGFSQIIRKYRLFEHKKNLNEVFELCSSLFSK
                     DKKDSIFSAAEKCAKKHREKGVRWIHYYSEEYPELLRFTDDPPPVLYCRGNIELLKKS
                     ITAAVGTRKPSPFAVSSCCKIAEYIGTQSDACISSGLAEGIDRHIMNLSLEYNISVIG
                     VMGTGFDKEYPSSNSDLYRKMKMSGNALILTEIQWGENPGKWSFPKRNRIISGISERV
                     IIIECPEKSGAVSTAAHAAAQNRDLIVFDAPELLNNAGGRKLISEGAKKISPEDIGSG
                     RVFHISELYKLPKERSSESLSRLARLEFSGGVKDLGGGYFEITDTDEVFHK"
     gene            complement(1226..2185)
                     /locus_tag="PK683_08435"
     CDS             complement(1226..2185)
                     /locus_tag="PK683_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08516.1"
                     /translation="MKFYLTNSVFISKKLSLILILLFFSCDKLKELGRENIRKKLSEP
                     NISPKEMERWKEQLGIEEAEIVRLDEQIRKMVKKTKEAGALSWKIARAYMKTGSYHYA
                     AMYYDRAIHEKQGKDLSNEPPSAELHSFESAIPFFEKTALYKDIDEELLFETGLAYAN
                     ASKDRGWDKQKRSAAISIFKGLTRKNPEDLRYPYELALIYFDSSMNDGLIDGVDPEGY
                     NDSEKAMKILYAILNRYSERAVNAESKSLQQEAMGAGVSVRFTLANFLYRQGKSSEAE
                     QEYVKIKTILEKFKEEGRITENLEKNSSYMNVIKNLKKIQETR"
BASE COUNT          614 a          494 c          438 g          798 t
ORIGIN      
        1 gcagccggca agaaaagacg caattcttta tgaatatcag tatatctgtt atactctttt
       61 tgcatacggt gagtttatca gaattatcct tgcccttctt ttttatttga aatctgttga
      121 attgataaca gaaacttatt gcttgattaa ttttttataa ttattaatta attatatatg
      181 caaatgaagt tcggagctgt tgctccgaaa tcagattcat ttttcgtgaa tgacccgtct
      241 atttatgaaa tacttcatcc gtatctgtaa tttcaaaata cccgcctccc aaatccttga
      301 cacctcctga aaactccagt cttgcaagtc tggaaagcga ctctgaggat ctttcctttg
      361 gaagcttgta aagctcactg atatggaaca ctctccctga gccaatgtcc tccggagaaa
      421 ttttttttgc accctcggaa atcagttttc ttcccccggc attattcaac agttcggggg
      481 cgtcaaaaac aatcagatct ctgttctgtg ccgcagcgtg tgctgctgta gacactgcac
      541 cggatttttc cggacactct atgatgatga ctcgctcgga tatgccggag atgattctgt
      601 ttcgttttgg aaatgaccat ttccctggat tttctcccca ctgaatctca gtgagaatca
      661 gtgcatttcc cgacattttc atctttctgt acaggtctga atttgaggaa ggatactcct
      721 tgtcaaatcc agttcccatt actccgatga cagaaatatt gtattctaat gataaattca
      781 tgatatgtcg gtctatgccc tctgcgagtc cggaggaaat gcaggcatct gactgtgttc
      841 cgatgtattc tgcaattttg cagcagctgg atactgcgaa cggagacggt tttcttgtgc
      901 cgacagcggc tgttattgat tttttcagaa gttcaatatt gcctctgcag tatagaacag
      961 gaggaggatc atctgtaaaa cgcagaagtt caggatactc ttcggaatag taatgaatcc
     1021 atctgactcc tttttctctg tgtttttttg cgcatttttc ggctgctgaa aaaatagaat
     1081 cttttttgtc tttgctgaac agagacgagc agagttcaaa aacttcattc agattttttt
     1141 tatgttcaaa aagtctgtat ttgcggatga tctgtgaaaa accggaagag gaaagagaat
     1201 gaattctgat atcctcttcc aaatgctacc tcgtttcctg aatttttttc aggtttttga
     1261 ttacattcat ataggaggag tttttttcca aattctctgt gattcttccc tcttccttga
     1321 atttttcgag gatagtctta atctttacgt attcctgttc tgcttcagat gacttgccct
     1381 gtctgtaaag aaaattcgcc agagtaaaac gaacggaaac gccagccccc attgcctcct
     1441 gctggagcga tttgctttct gcatttactg ctctttcact gtatctgttc aaaattgcat
     1501 agagaatttt cattgccttt tctgaatcat tgtatccttc aggatcgacc ccatcgatca
     1561 gtccatcgtt catggaagag tcaaaataaa tcagagccag ctcataagga tatctcagat
     1621 cctccggatt ctttctggtc agacctttaa aaatactgat agcagccgat cttttctgct
     1681 tgtcccatcc tctgtcttta gatgcgtttg cataggcaag acctgtttca aaaagcagtt
     1741 cttcatctat atccttataa agagcagttt tctcaaagaa cggaattgcg ctttcgaaag
     1801 aatgcagttc tgcagaggga ggttcattcg aaagatcttt tccctgtttc tcatgaatgg
     1861 ccctgtcata atacattgca gcataatgat aacttccggt tttcatatag gctctggcaa
     1921 ttttccacga gagagcacct gcttcttttg tttttttcac catcttgcgg atctgttcat
     1981 ccagacgcac aatttctgcc tcttctatcc ccagctgttc tttccagcgt tccatttcct
     2041 tcggggaaat atttggttca gagagttttt tgcgtatgtt ttctctgcca agctctttca
     2101 gtttgtcgca ggagaaaaaa agcagaatga gtatcagtga aagtttttta gagataaata
     2161 cggaatttgt aagataaaat ttcataagat aatgttcgga acctctttcc cgcaggcaac
     2221 caatttactt tccaaaataa cctgtctgaa aatgttcagc acggaagatt tttatttcat
     2281 atttgcatct tacagtaaat tttactttca gaatgcaata tgactgttca gcagaaaata
     2341 taag
//
LOCUS       DAOQGJ010000474         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29693_length_2344_cov_2.619921, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000474 DAOQGJ010000000
VERSION     DAOQGJ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29693_length_2344_cov_2.619921"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..626
                     /locus_tag="PK683_08440"
     CDS             243..626
                     /locus_tag="PK683_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH08517.1"
                     /translation="MLQIVFQCSRILSRFPAILLIFGSSYLYAVAPSDYLLFSSERGY
                     IGHVKKALAQGADVNVQDYFKKTPLIYASSYGDIEIVKLLLKYGAAEGIDIRDREGKT
                     AYTYAKLKGFTQIMNLLKQYGASEE"
     gene            628..1611
                     /locus_tag="PK683_08445"
     CDS             628..1611
                     /locus_tag="PK683_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08518.1"
                     /translation="MIKKIMMIAFCAGAVNCSMLDSMAGGLAKPSFSFQKLDIKNITL
                     SEITFNLLTSVKNPYPVSLPKSNLNMNLMIEGTTFLKDVKTDMGKVEAGSVKDLPFEF
                     KLSYDQLQQLYKKFPSKELLDVKLDGIMGLPIPDKFQLAGKKSVDFPFAESRQVPAIL
                     PTVDIKNFQIMKPDTSKVMESAGSNQNLGQTAVGMLDKLLDGKPGTPSSAMSSALSGL
                     DMNIDTQFDIALKNLAAAPLNFSDLKYDLTLKGEKFVSGVPDQIINQGKESTVKVKSS
                     FPLKTLSAGLIDAIQKKNADFRLVGASGLKVPGLPDGILNFQYDKNGNFKW"
     gene            1583..2170
                     /locus_tag="PK683_08450"
     CDS             1583..2170
                     /locus_tag="PK683_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNH08519.1"
                     /translation="MTRTEILSGEQGSLIYCPGFFSTQDSGKFFKNLQTEIRWQENEI
                     TLFGKKIKEPRLTAFLGNEGIKYKYSGSLRSAEKWTDTLLEIKQKSENEFNSEFNCVL
                     CNLYRNGQDSMGWHSDDEKELGADPIILSLSFGAERKFLLKEKEGRNRISLNLKSGDA
                     LFMGRGIQRNWKHCIPKSIKSTERISLTFRAVCMK"
BASE COUNT          732 a          436 c          538 g          638 t
ORIGIN      
        1 ggaacaggac attcttgtcg atggaaagac agtaaaaagg tatcaggctt ggcagagggg
       61 gcgggtttta tgacagaact cttgagtttt ccgttctgga gcggaccctg ggactcggct
      121 ttaaggaact ttttccgata aattttactg cagagcctta tgatattgca gtgaatgcaa
      181 tagtatttga tgactgagta tttgtgcttg ctttttttta ctgcagagca cattctttga
      241 ctatgcttca aatcgtattt cagtgttccc ggattctttc aagatttccg gctatcctgc
      301 tgatcttcgg ctcaagttat ctgtatgccg ttgcaccgtc agactatctt cttttcagtt
      361 cagaaagggg gtatattgga catgtaaaaa aagcacttgc acagggtgct gacgtgaatg
      421 tgcaggacta ttttaaaaag acacctctta tatatgcaag ctcgtacggc gatattgaaa
      481 ttgtgaagct gcttctgaaa tacggagctg cagaagggat tgatattcgg gacagagaag
      541 ggaagactgc atatacttat gcgaaattaa aaggatttac gcagattatg aatctgctga
      601 aacaatacgg agccagtgag gaataaaatg ataaaaaaaa ttatgatgat tgcattctgc
      661 gcgggagcag tgaactgcag tatgctcgac agtatggctg gaggacttgc aaaaccttct
      721 ttctcatttc agaagcttga tataaaaaat attacactct ctgaaatcac tttcaatctt
      781 ctgacatcgg tcaaaaatcc ctatccggta agtctgccta aatcaaatct gaatatgaat
      841 ctgatgatag aaggaacaac ttttctgaag gatgtgaaga cagacatggg aaaagtggag
      901 gcaggttctg tcaaggatct ccctttcgag ttcaagcttt cctatgatca gcttcagcag
      961 ctgtataaga aatttccgtc aaaagaactt ctggatgtaa agctggacgg aattatggga
     1021 ctgcctatcc cggataaatt tcagctggcc ggaaagaagt cggtcgattt tccttttgcc
     1081 gaatccagac aggtgccggc catacttccg acagtggata taaagaattt tcagataatg
     1141 aaaccggata catccaaggt gatggaatcg gcagggagca atcagaattt aggacagact
     1201 gcagtcggga tgcttgataa actgctggat ggaaaaccag gaacaccttc gtctgcgatg
     1261 agttctgcac tttcagggct ggatatgaat atagatacac agtttgacat tgcactgaaa
     1321 aatcttgccg cagccccgct gaatttcagt gatctaaaat atgatcttac acttaagggc
     1381 gaaaaatttg tttccggggt tcctgatcag attatcaatc agggcaagga gtctacagtg
     1441 aaagtcaaaa gcagcttccc tctcaagact ctttctgcag gtcttatcga tgcgatacag
     1501 aaaaagaatg ccgacttcag actcgtaggg gcttccggac tgaaagtgcc cggacttccc
     1561 gacgggattc tgaatttcca atatgacaag aacggaaatt ttaagtggtg aacaaggttc
     1621 cctgatctac tgcccgggat ttttttcaac tcaggattcc gggaagtttt tcaaaaatct
     1681 gcagacagaa atcagatggc aggaaaatga aattacctta tttggaaaaa aaattaaaga
     1741 acccagactc accgcatttc tcggcaatga aggaataaaa tacaagtatt caggatctct
     1801 cagatccgct gaaaaatgga cagatactct tctcgaaatt aagcaaaaat cagagaatga
     1861 attcaattcc gaattcaact gtgtcctctg caatctttac agaaacggtc aggacagtat
     1921 gggatggcat tccgatgatg aaaaggaact tggtgcagat cccattatat tgtcattaag
     1981 ttttggtgca gagagaaaat ttctcctgaa agaaaaagaa ggcaggaatc gaatcagcct
     2041 gaatctgaaa agcggcgatg cgctgtttat gggaagaggc atacagagaa actggaaaca
     2101 ctgcatccct aaaagcatta aatcgactga gagaatcagt ctcacattca gagccgtctg
     2161 tatgaaataa aatttttgtt tgacaccaag aaatataatt aatactgtta gtttcaggag
     2221 gcttaaatgc tcggattttt tacaggtctg gtttatgcca atgcatggga atggatgatt
     2281 cacaagtatc ttctgcatga agaaggacgg aaaaaagaca gtttctggag attccactat
     2341 atgg
//
LOCUS       DAOQGJ010000475         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_29777_length_2340_cov_3.709847, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000475 DAOQGJ010000000
VERSION     DAOQGJ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29777_length_2340_cov_3.709847"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK683_08455"
     CDS             complement(<1..297)
                     /locus_tag="PK683_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08520.1"
                     /translation="MRTPGKNSTDPFCSRENRRTGGLLDIFRIFLYIITYTVITNCFL
                     WTPTRMIPAKPSPVHEKPSSRLKVYWIGHATMLVRMEDRWILTDPNWSLRILTLG"
     gene            complement(311..937)
                     /locus_tag="PK683_08460"
     CDS             complement(311..937)
                     /locus_tag="PK683_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08521.1"
                     /translation="MKEWKTTLFCGSFEGETDMFSRIDGLKVYNTGIGLHDSLYSLQR
                     YLMKNQDIKTVLFFGSAGAYPHSELKVGNIVYSYKFLSKELAEIKNMCKVPEIMTRHI
                     LTQVDPRTADMIEKLGFKEVISNSMNYVTLVNLSSEELVNSLFDVGAENMEAFSLAYA
                     ASRLSVNFTAFYYITNIVGESGSKDWAIHWREGSNILQRKIINYIKGK"
     gene            complement(934..1944)
                     /gene="argC"
                     /locus_tag="PK683_08465"
     CDS             complement(934..1944)
                     /gene="argC"
                     /locus_tag="PK683_08465"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNH08522.1"
                     /translation="MKIAIIGAGGLTGRELLSLLKNHSGFELAHITSSKTEGKTISEV
                     FPNLNIKSGLKFKKHEEEIPKDCLVVLAVPNEVSLEMTPKLLDKGHRVIDLSGAYRLH
                     NQSEFEKYYNLKHESFSLMEKAVFGIPEIFREKIKNAALVSNPGCYATSVILPLYMLG
                     DLRKKILGSPVVDSKSGVSGAGGRTEDIGFTFTNTYENFRAYKILSHQHVPEMQEYAG
                     TGLSKFPKIIFTPHLLPVYRGILSTFAVQFTENVSEKEITEKFSLFQNEPFIRIYRKP
                     EEIELKNVQNSNFLDISFRSEENILVIVSALDNLLKGAAGQALQNMNLMAGFNETEGL
                     LH"
     gene            2173..2298
                     /locus_tag="PK683_08470"
     CDS             2173..2298
                     /locus_tag="PK683_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08523.1"
                     /translation="MGLAEKKATPIEFDYLESDRNSEQKFEFLYGEMDAMTGLLD"
BASE COUNT          647 a          480 c          480 g          733 t
ORIGIN      
        1 tcccagtgtc aggatacgga gactccagtt cggatctgta agaatccatc tgtcttccat
       61 ccggacaagc attgtagcgt gtcctatcca gtaaactttc agtctggatg agggtttttc
      121 atgaacagga cttggctttg ccgggatcat tcttgtcggc gtccacagaa aacaatttgt
      181 aatgacggtg tatgtaataa tatataaaaa tatcctaaaa atatccagaa gtcctccggt
      241 tcttctgttt tctctgctgc agaaaggatc tgttgaattt tttccgggag ttctcattgt
      301 tcggttcttt ttattttccc tttatgtagt tgatgatttt tctctgaaga atatttgagc
      361 cttccctcca gtgaattgcc cagtccttgg acccgctctc acccactatg tttgtgatat
      421 aataaaatgc agtaaaattg acggaaagtc tgcttgcagc gtatgccaga gaaaaggctt
      481 ccatattctc tgcgccgaca tcaaacagac tgttgacaag ctcttcagag cttaaattta
      541 ccagtgtaac gtaattcata gaattggaga tgacttcttt gaagcccagt ttttcaatca
      601 tatctgcagt tctggggtca acctgagtca ggatatgtct tgtcataatt tctggaacct
      661 tgcacatatt cttgatttca gcaagttctt ttgaaaggaa tttatacgag tatactatat
      721 tgcccacttt cagttcagag tgaggatatg cgcctgcaga tccgaagaag agcactgtct
      781 taatatcctg atttttcatg aggtatcgct ggagtgaata cagtgaatcg tgaagaccga
      841 tgcctgtatt ataaaccttc aggccgtcta ttctgctgaa catatctgtt tcaccttcaa
      901 aggatccgca gaaaagagtg gttttccatt ccttcaatga agaagtcctt ctgtttcatt
      961 gaatccggcc atgagattca tgttctgcag agcctgcccg gctgcccctt tcagaagatt
     1021 gtccaaagcg gaaacaatga cgaggatgtt ttcttccgat ctgaaactga tatccagaaa
     1081 attggaattc tgtacattct tcagttctat ctcttccggt ttgcgatata ttctaatgaa
     1141 aggttcattt tgaaacaggg aaaacttttc ggttatttcc ttttcgctga cattttcagt
     1201 gaactgcacg gcaaatgtgg acagtatccc cctgtataca ggaagcaggt gaggggtaaa
     1261 tatgatcttc ggaaattttg aaagtcctgt tccagcatat tcctgcattt caggtacatg
     1321 ctgatggctt aaaattttat aagctctgaa attttcatat gtatttgtaa atgtaaaacc
     1381 gatgtcctca gttctccctc ccgcaccgct gactcctgat tttgaatcga caacaggaga
     1441 tccaagaatt ttttttctaa ggtcgccaag catataaagg gggaggatga ccgatgtcgc
     1501 gtaacatccc ggattggata caagagcagc gtttttaatt ttttcacgga aaatttcagg
     1561 aattccgaat actgcttttt ccataagaga aaaagactca tgtttcagat tataatattt
     1621 ttcaaactca gactgattat gcagccggta ggcccctgaa agatcgatta ctctgtgtcc
     1681 cttgtccaga agtttcggag tcatttcgag ggacacttca ttcggaacgg ccagtacaac
     1741 caagcagtct ttcgggattt cctcctcgtg ctttttgaac ttcagaccgg attttatatt
     1801 cagattagga aacacttctg aaatcgtttt cccttccgtc ttgctgcttg tgatatgagc
     1861 aagttcaaat cctgaatgat ttttcaagag ggacaaaagt tccctgccag tcagtccgcc
     1921 cgcaccgatg attgcaattt tcatattttc aaaattcccg cttgtacaaa ttgagtcaaa
     1981 cggaaaatcc tcagaattga ctttgtaaaa acaaccttca tcatttttaa tgaaaacttc
     2041 actgacccgg acggaaatcc tgtcataatc cgagagtgtt gcatttggaa tggaagcatc
     2101 aggcgatttt atactgcagg tgaaaagtca aaatcagctt gaggttattc ctctcggata
     2161 aaaactgtaa ctatgggtct ggcggaaaaa aaagcgacac ctattgagtt tgattatctg
     2221 gagtctgaca gaaattcaga gcaaaaattc gaatttttat acggagaaat ggatgcaatg
     2281 acagggctgc ttgattgaca ctggtaataa gtttaatgat tcgactgcag gcggaatgcc
//
LOCUS       DAOQGJ010000476         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30033_length_2326_cov_4.019375, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000476 DAOQGJ010000000
VERSION     DAOQGJ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30033_length_2326_cov_4.019375"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK683_08475"
     CDS             <1..313
                     /locus_tag="PK683_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08524.1"
                     /translation="MELTVDFHHETKVSHFRGLVGNYHSEGAEETPEVITVSGKKHTK
                     LGFKFSVKWDEYRLPFDLGLKKIVYQIFTGTLIGNVVDSTIGFTFNLLKKLVSPSSDS
                     Q"
     gene            complement(314..1615)
                     /locus_tag="PK683_08480"
     CDS             complement(314..1615)
                     /locus_tag="PK683_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HNH08525.1"
                     /translation="MKNLFALFSILFLNFCMPVISRDRNPSSGKKLIVLSIDGFPGYY
                     LKEGSAVLQIMPNLKKLLSRSVHSNQVSTVYPSLTFPAHTSMITGADPAKHGIFYNNP
                     PDPFKKLEGDWYFFREDIKTLTLFDYMKRSGKKTASLYWPVTVGADIDWNIPQYWKNK
                     TKYDEKYLRALSTPGLYSELHRKTGSTVGEFTGDEEKVMSAIAVWQHYSPDFMTVYTT
                     DLDTAHHVSGVSSEAAMQKISNIDRLLGKLISAVRLYERDDVGLLVTSDHGFKEVNAV
                     CYPNKVLMDTGAADTDTGKWSYLFKSLGGTAVLLKNPDRKSRKNFSLDMDDLRKKIEG
                     GCENVILADSGDKFKTARKKLSSEIEAFLYTEKNTAFSESLGAKELFKQTGTYYNHGF
                     LPEDSDLKTIGIFYPANEEYRMNTVKDVFVNSCRWMNVACR"
     gene            1842..>2326
                     /locus_tag="PK683_08485"
     CDS             1842..>2326
                     /locus_tag="PK683_08485"
                     /inference="COORDINATES: protein motif:HMM:NF039306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HNH08526.1"
                     /translation="MNRKNGIRSSVLLCRLIFCFLISSLNISAEAGKPERKRPDSLPF
                     DVSEEQRISEDDLKNKPEGWYPVMLPVIAKDETLGNFYGANLNFVNNGSRANPLFEYT
                     PYRDSLNLAFIYSDLGMRGYYFDYLKKYIKDTPYSVQFSGGYQKITNFLYMGIGAETM
                     NS"
BASE COUNT          650 a          477 c          490 g          709 t
ORIGIN      
        1 tatggaactg actgtggatt ttcatcatga gacgaaggtt tctcatttca gaggtcttgt
       61 gggaaattac cattcagaag gagcggaaga gactcctgaa gtcattacag tatcaggaaa
      121 aaaacataca aaacttggat ttaaattttc agtcaagtgg gatgaataca ggctgccgtt
      181 cgatcttgga ctgaaaaaaa ttgtgtacca gatattcacc ggtacactga tcggaaatgt
      241 tgtggacagt acgatcgggt ttacattcaa tctgctgaaa aaacttgtca gtccttcttc
      301 ggacagtcag tgactatctg caggcaacat tcatccatcg gcatgaattg acaaatacat
      361 cttttactgt gttcattctg tattcttcat ttgcaggata gaatatccct atagtcttca
      421 aatcgctgtc ttccggaaga aacccatgat tgtagtatgt tcctgtctgc ttgaaaagtt
      481 cttttgcacc gagagattct gaaaaagcag tattcttttc agtatataaa aatgcttcaa
      541 tctcagagga aagttttttc ctggcagtct tgaatttatc tccgctgtcg gctagaatga
      601 cattttcaca tcctccctca attttttttc tcagatcatc catatccaga gaaaagtttt
      661 ttctgctctt cctgtcggga tttttcagaa ggacagctgt tcctccgagg ctcttgaaca
      721 gataggacca ttttccagta tctgtgtcgg cggcacctgt atccatcaga accttgttcg
      781 gataacagac ggcattcact tccttgaatc cgtgatccga tgtcacaaga agaccgacgt
      841 cgtctctttc atacagcctt actgcggaaa tcagttttcc aagtagtctg tcaatattgg
      901 aaatcttctg cattgccgcc tctgaactga ctcctgaaac atggtgggca gtgtcgagat
      961 ctgttgtata cacagtcata aaatcaggac tgtaatgctg ccagactgca attgcagaca
     1021 tcactttttc ttcgtctcct gtgaactcgc ctactgtgga gccggtcttc ctgtgaagtt
     1081 cagaataaag tcctggagtc gataatgccc gaagatattt ttcatcatat tttgtcttgt
     1141 ttttccaata ctgaggtatg ttccagtcaa tgtccgcacc gactgtcaca ggccagtaaa
     1201 gtgaagctgt cttttttcct gatcttttca tataatcaaa taaagtaagt gtttttatat
     1261 cttctctgaa aaaataccag tcgccctcca gttttttgaa agggtcaggg ggattgttgt
     1321 agaatatccc gtgttttgcg ggatcggctc ctgtaatcat ggaagtatgt gcaggaaacg
     1381 tgagagaagg gtatactgtg gaaacctgat tggaatgaac ggatcggctc aataatttct
     1441 tcaggttcgg cataatctgc agaacggcag agccttcctt cagatagtac ccgggaaaac
     1501 cgtcaatgga aagaacgatg agtttcttcc ccgaagaagg atttcggtct ctcgatatga
     1561 caggcataca gaaattcaga aacagaattg aaaacagtgc aaatagattc ttcatacatt
     1621 caggaaaatg aaacgccctt cagggtcaat acaatccgat actgcttatc ctcgcttctg
     1681 cagtctgaaa gctttgattt tcttcatcgg aatcacagtc gtccgaatct cccgctgtca
     1741 aatgaagcct ttggagcttt gacagttttc ggcttatcac ttgaattgct ttactttcag
     1801 tccgctgcag aaagaaatgc agttctggac gtcatttgaa aatgaatcgg aagaatggaa
     1861 tacgaagttc agttttactg tgtcggctga ttttctgttt tcttatatcc tctttgaata
     1921 tttctgcaga agccggcaaa ccggaaagaa aaagacccga cagtctgccc ttcgatgtat
     1981 ctgaagaaca gagaatttcg gaagatgacc tgaaaaacaa gccggaggga tggtatcctg
     2041 tcatgcttcc tgtgatcgca aaggatgaaa cactcggaaa tttttacggt gcaaatttga
     2101 atttcgtcaa caacggaagc agggccaatc ctctctttga atatactccg tacagggatt
     2161 ctctgaatct tgcattcatt tattccgatc tgggaatgag aggatactat tttgattatc
     2221 tgaaaaaata cattaaggat acaccgtatt ctgttcagtt ttcaggaggt tatcagaaga
     2281 ttacaaattt tctgtatatg ggaatcgggg ctgaaacaat gaattc
//
LOCUS       DAOQGJ010000477         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30056_length_2325_cov_3.352423, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000477 DAOQGJ010000000
VERSION     DAOQGJ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30056_length_2325_cov_3.352423"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PK683_08490"
     CDS             <1..246
                     /locus_tag="PK683_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HNH08527.1"
                     /translation="DVPSYVLIPAFMLSELKKAFIIGIYLFIPFIIIDIVVASTLLSM
                     GLMMLPPVMISLPFKLILFVLVDGWNLLVYELVRSYK"
     gene            243..506
                     /gene="fliQ"
                     /locus_tag="PK683_08495"
     CDS             243..506
                     /gene="fliQ"
                     /locus_tag="PK683_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNH08528.1"
                     /translation="MTELDAITLLRDAIFVTLKISSPILITALIVGFVIGVLQTTTSI
                     QEPTIAFVPKLIAIFVVIVVFSGWIIKVLTDYTRDIFMMIEKI"
     gene            complement(519..2132)
                     /locus_tag="PK683_08500"
     CDS             complement(519..2132)
                     /locus_tag="PK683_08500"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH08529.1"
                     /translation="MIPKASYRQTICFPLAFTDKSEGYGIMKNLPIGRQSFKDIRERN
                     EVYVDKTRYLYALVKNGGVFFLSRPRRFGKSLLISTLNEIFLGNKEAFSGLWMENSDY
                     DWKKYPIIRIDFSKQKAKNTEQLERFIKEQLDYIAEDYGIRLTHQEYFSRFDQLIQLL
                     SKESKIVILIDEYDKPIIDHLENPELAKEMREILKGFYTVIKAGDENLRFVLLTGVSK
                     FSKAGVFSGLNNLNDISMDNKYASILGLTENEITDNFSGYLEIFAAEKNISVEDLIQK
                     IRAWYNGYLFAGIGERVYNPFSTLLLFDKNEFRNYWFETGTPSFLVNILKQTDFPLSS
                     LESAEAEAEKFSVYDIENLDPIPLLYQTGYLTIKEYSEEFGSYILGYPNFEVKNSFTK
                     ALASAFSKNHSDMPINSLIRSLRDRDWETFFHGLREIFLKIDYDLQINQEKYYQTIFY
                     LIFTLIGLKTSAEVKTNIGRIDTVIDDRDILIFEFKFSSTGAGAQDALNQIKSKKYYE
                     KYQSSSKEIFLFGAEFRDRNIGEWIFEKV"
BASE COUNT          627 a          485 c          438 g          775 t
ORIGIN      
        1 gatgttccgt cctatgttct gattcccgca ttcatgcttt ccgaactgaa aaaagcattc
       61 atcatcggaa tatatctttt tatacctttc atcatcatag atattgtagt ggcatccact
      121 ctgctttcga tgggacttat gatgcttcct cccgtgatga tcagtcttcc gttcaagctg
      181 atcctctttg tgctggtgga cggatggaat cttctggtat atgaacttgt gaggagctac
      241 aaatgacgga actcgatgca atcactctcc ttcgggatgc gatttttgtg acattgaaaa
      301 tctcctcccc catcctcatc actgccctca tcgtgggatt tgtgatcgga gtgctccaga
      361 ccaccacttc cattcaggaa cccacaattg catttgttcc taaactcatc gctatttttg
      421 tagtgattgt agtcttttcc ggctggataa taaaggttct tacggactat acccgcgata
      481 tttttatgat gatagagaag atttagattt tcgagttttc agactttttc aaagatccat
      541 tctccgatat ttctgtctct gaactctgct ccaaatagaa aaatctcttt ggaagaggac
      601 tgatattttt cgtaatactt tttagattta atctgattga gagcatcctg cgctccggct
      661 ccagtactgc tgaatttgaa ctcaaagatc agtatatctc tgtcatcaat cactgtgtct
      721 attctgccga tatttgtttt tacctctgca gaagttttca gcccgattaa tgtgaatatc
      781 agataaaata ttgtctgata atatttttcc tgattgatct gcagatcata gtcaattttc
      841 agaaatatct ctctgagtcc gtgaaaaaac gtctcccagt ctctgtctct caggcttctg
      901 atcagtgaat tgatcggcat atccgaatga tttttggaaa atgcagatgc gagagctttt
      961 gtgaaagaat tcttcacttc aaaattagga tatcctaata tataacttcc gaattcttct
     1021 gaatattcct taatggtcag ataaccagtc tgatacagaa ggggaatcgg atccaggttt
     1081 tcaatgtcat acacggaaaa tttttcagcc tcggcttccg ctgattccag actggaaaga
     1141 gggaaatctg tctgtttgag aatattgact aaaaaactcg gtgtgcctgt ctcaaaccag
     1201 taattgcgaa attcattttt atcgaaaagg agcagtgtgg aaaaaggatt gtaaaccctt
     1261 tcaccgatgc ctgcaaaaag ataaccgttg taccaggccc ggattttctg aatcagatcc
     1321 tccacggaaa tatttttttc tgcagcaaaa atttccagat agccgctgaa attgtctgta
     1381 atctcatttt ctgtaagccc gaggattgat gcatatttat tgtccataga aatatcattc
     1441 agattgttca acccgctgaa aacacctgcc ttggagaatt tggacactcc agtgagcagc
     1501 acaaaccgca ggttttcgtc accggctttg atgacagtat aaaatccttt gagaatttcc
     1561 cgcatttctt ttgccagttc cggattttcc agatggtcaa tgatcggttt gtcatattca
     1621 tcaatcagaa tgacaatttt gctttccttg gaaagaagct gaatcagctg atcaaaacgt
     1681 gaaaagtatt cctgatgagt cagtctgatt ccatagtctt ccgcaatata gtccagctgt
     1741 tctttgataa accgttccag ctgttccgta tttttcgctt tctgtttgga aaaatcaatt
     1801 ctgatgatgg gatatttttt ccaatcatag tcagaatttt ccatccagag tcctgagaag
     1861 gcttctttgt ttcccagaaa gatttcattg agagtggata tgagaagact tttgccgaat
     1921 cgtctcggac gggaaagaaa gaatacgcct ccgtttttta ccaaagcata caagtaacgg
     1981 gtcttatcca catagacttc atttctttcg cgtatgtctt tgaatgactg tcgtccgatc
     2041 ggcagattct tcataattcc ataaccttcc gatttatctg taaaagcaag aggaaaacag
     2101 atggtctgtc tgtaagaagc tttggggatc attcatctgt gaaaaaataa gttgaccgaa
     2161 ataatttctg aataatacag tgtccagcaa gaccggccgt gttagaaatc tttgtttaca
     2221 atttccagac ttttatgatg attttttcca ggatcatcgc gctgttcttt attgctccta
     2281 tcttttcttc cgatacaatc agttatcctt tccgaatatt tctgg
//
LOCUS       DAOQGJ010000478         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30141_length_2321_cov_3.229479, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000478 DAOQGJ010000000
VERSION     DAOQGJ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30141_length_2321_cov_3.229479"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /gene="pheA"
                     /locus_tag="PK683_08505"
     CDS             complement(<1..991)
                     /gene="pheA"
                     /locus_tag="PK683_08505"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNH08530.1"
                     /translation="MNEDLKDLRDSIDSLDQEIVNKIQARAKLASQIGEIKRAKGEPI
                     YRPDREKDVYEKIKRINPGPLPDSAMTSIYREIMSASIAIEKGLTIGYLGPEGSFSHQ
                     AVRARFGASVQSMQYSSIPEVFRSVETGKCDYGVVPIENSSEGLVNSTLDMFLMSDLQ
                     IYSEIYIKISICLIGFENDLKKINTLYGIKIANSQCKNWIAANLPNVTIIETSSTAKA
                     AEIVAEKKEGGAIASKIAAEIYGLNVIRENIEDLTNNSTRFLIIGKSQCLPTGKDKTS
                     IVFSIPDKAGSLFNALKPFYENKINLTKIESRPTRRNLWQYNFFIDFYGHEKEP"
     gene            complement(984..1565)
                     /gene="scpB"
                     /locus_tag="PK683_08510"
     CDS             complement(984..1565)
                     /gene="scpB"
                     /locus_tag="PK683_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNH08531.1"
                     /translation="MEEEKSKAYWKGLVEALLFLSAEPVKLAAVADSAGIEKTLARDL
                     IEELILDYEEKSGGFVLKEVAGGYQFFTNELYHHELGKIFKEKRRETLSKSTLDTLAI
                     IAYKQPITLPEIDEIRGVSSRAMVTTLISKKLVKAIGQKEVPGRPTLYGTTQDFLAHF
                     GLSKLSDLPTPMEVKELNFDDIDDLSRENQENE"
     gene            1777..>2321
                     /locus_tag="PK683_08515"
     CDS             1777..>2321
                     /locus_tag="PK683_08515"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH08532.1"
                     /translation="MTNLAVKLMTESEYLEMERNSDSKHEFYQGEIFAMAGAKRVHNQ
                     IVFNISGLLFNHFREKNCNAYGSDMRVQVSTNGLFTYPDIVVVCGEEKYLDKTEDTFL
                     NPSLIIEVLSDSTENYDRGKKFALYRGLESLKEYMLVSSEYRKVEIFRKNGAGQWVLS
                     DVNEDEPAVLESVGCSLLLND"
BASE COUNT          596 a          461 c          500 g          764 t
ORIGIN      
        1 gcggttcttt ttcgtgtccg taaaaatcta tgaagaaatt gtactgccag agatttcttc
       61 tggtcggtct ggattcgatt ttggtcaggt tgattttatt ttcataaaac ggtttcagtg
      121 cgttaaacag tgatcctgcc ttgtccggaa tggagaatac aatggatgtc ttgtcctttc
      181 ctgtcggcag gcactgtgat tttccaataa ttaaaaacct tgtggaattg tttgtcagat
      241 cctcaatatt ttcgcggatc acattcagtc cgtaaatttc agcagcgatc ttggaggcaa
      301 tcgcaccgcc ttcttttttc tctgctacaa tctctgctgc ttttgcagtg gaagatgttt
      361 ctatgatggt tacattcgga aggttagcgg ctatccagtt tttgcactga gagttggcta
      421 ttttgattcc gtacagagta tttatttttt tcagatcgtt ttcaaaaccg atcagacaga
      481 ttgatatctt gatgtaaatt tcagaataga tctgcagatc agacatcagg aacatatcca
      541 gagtggaatt gaccagcccc tcactggagt tctcaatcgg tacaactcca taatcgcatt
      601 tccctgtttc tacagaccga aacacttcag gtattgaaga atactgcatt gactgcacgg
      661 atgcaccgaa tcttgctctg actgcctgat gagaaaaaga accttccgga cccagataac
      721 cgattgtcag ccccttttca attgcaatcg aggcagacat aatttctctg taaatggaag
      781 tcattgcaga gtctgggaga ggacccggat tgattctttt gattttttcg tagacgtcct
      841 tttctctgtc aggacggtag ataggttccc ctttagctct tttgatttcg cctatctggg
      901 atgcaagttt ggctcttgcc tggattttat ttacaatttc ttggtctaga ctgtcgatgg
      961 aatctctcag gtctttcagg tcttcattca ttttcttggt tttctctgct caaatcgtca
     1021 atatcgtcaa aatttaattc ttttacttcc attggagttg gcaagtcaga aagtttcgat
     1081 aatccaaaat gtgcgagaaa atcctgagta gttccgtaca gagtcggtct gccgggtact
     1141 tctttctggc cgatagcttt gaccagtttt ttggagatca gagttgtcac cattgctctg
     1201 gaggaaaccc ctctgatttc atcaatttcg ggaagagtga tcggctgttt gtatgcaatg
     1261 atggcaagtg tgtcgagagt gctctttgaa agagtttccc ttcttttttc tttgaatatt
     1321 tttccgagtt cgtgatggta caattcattt gtgaaaaact gataacctcc ggcaacttct
     1381 ttcagtacaa agccgcctga tttttcctcg taatcgagga tcagttcttc aatcagatct
     1441 cttgccaagg ttttttctat cccggcagag tcggccacag ccgccagttt caccggttct
     1501 gcactcagga aaagcagtgc ttccactaaa cccttccaat atgccttgct tttttcttct
     1561 tccaacgtag ctgctcagct taaatataaa gttaagaaaa ggcagggatt ttcagccagc
     1621 tttccctgca attccttgta ccgttctagg ttttagaaag gcagaagggg atcaatcgaa
     1681 atttttgagg attattgtct ctttacgaat tcggataata actctgttcg gtgtcttttc
     1741 tcttgcaatt ttggatttct gtacaatcct tgcgttatga cgaatcttgc agtgaaactg
     1801 atgactgaat ctgaatattt ggaaatggaa agaaattcag acagcaagca tgaattttac
     1861 cagggtgaaa ttttcgcgat ggcaggcgca aaaagagtac ataatcagat tgtattcaat
     1921 atttctggat tgctttttaa tcatttcagg gaaaaaaact gcaatgcata tggttccgat
     1981 atgagagtgc aggtcagtac aaacggattg ttcacttatc ctgacattgt agttgtctgc
     2041 ggcgaagaga agtatctgga taaaacagaa gacacttttc tcaatcccag tcttatcatt
     2101 gaagtgcttt ccgattctac ggaaaactat gacagaggaa aaaagtttgc attgtaccgc
     2161 ggactggagt ctctgaagga gtatatgctt gtatcttcgg aatacagaaa agtggagatc
     2221 ttcagaaaga atggagctgg acaatgggtt ttgtccgatg taaatgaaga tgagcctgca
     2281 gttctggaat cagtcggatg cagtcttctt ctgaatgata t
//
LOCUS       DAOQGJ010000479         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30166_length_2320_cov_2.977042, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000479 DAOQGJ010000000
VERSION     DAOQGJ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30166_length_2320_cov_2.977042"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PK683_08520"
     CDS             <1..639
                     /locus_tag="PK683_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08533.1"
                     /translation="SKRLSKEAKASRHIFGLTPTILMFAKRLGFTIVSYDRIHLDENG
                     SVAEGSNASDRIPGIRIRCFQKGDSVLRDILYLKIFLKKDSHLPVTPEGRFFNSLKNI
                     NLTLKSAIYVLQTAEYEPFSRLIAERAETVFEDDSGVAVKFFNTPEWERKVFGYYKGR
                     IRVKDTPDVPSQKELIEDFSKKPGELPFPFGYGILQGVGRSNFVFMQKKKNN"
     gene            complement(642..>2320)
                     /locus_tag="PK683_08525"
     CDS             complement(642..>2320)
                     /locus_tag="PK683_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08534.1"
                     /translation="LNYYRTGKKDDAAVKLTNSPTHQWFFRTLSQGKKASLNIDTNTA
                     DGRTSLFVNAVMGDLSNPLGAAGVVMDFSHVRKEFTAVDSEISDRIHLADRQGVIKIS
                     SVNAMLNRNISEFYPPETAGIIMNNDQKRDILEYTDERGLMDVVKVPLKSAEWNVVYE
                     VPRESMIRPIFIIASGTLIICILSVITAAAVFMKAALSVTRPVRQLSSLTRDMSEGRL
                     YRKMESTSSDEIGLLGKTFNRLNDKLITVIDSVSQSSRHLKNSADTMSKATRNFSDNM
                     HSQVAALEEISSSVQSLSKNVSVVTRNAENQSDDLNSLAGSFRQLAGIITETERVVRY
                     SLQSTGRIYQDTKSGEASLVVLNSGMDSIVQGSNDMTKILGLISEISEKINLLALNAS
                     IEAARAGKAGLGFAVVAYEISKLADETAASLKEIDVIIRRSRKEVEKGKLSVSEMMKN
                     NSGTLEGVSAIVDLMTNMNDLIGKQVLMKENVEREAGNVMSKAEEIKLTVKDDERAFE
                     EISKGIKMIHEIAHSNLENSSTIADNSLSLFKIADELNSKIRFFRTKEKE"
BASE COUNT          590 a          526 c          466 g          738 t
ORIGIN      
        1 agcaaaagac tttccaagga agcaaaagcc agcaggcaca ttttcggact gactcctact
       61 attctgatgt ttgcaaaaag actgggcttt acaatagtga gttatgacag gattcatctc
      121 gatgaaaacg gttctgttgc agaaggcagc aatgcctctg acaggattcc aggcatacgg
      181 atcagatgct ttcagaaagg agattctgtt ctgcgggata ttttatattt aaaaatattt
      241 ctaaaaaaag actctcatct tcctgtgaca ccggagggaa ggttcttcaa ttcactgaaa
      301 aacatcaatc ttactctgaa atcagcgatt tatgtgcttc agactgccga gtatgagcct
      361 ttttccaggc ttattgcgga aagggcagag acagtatttg aggacgattc aggagttgcc
      421 gtaaaatttt tcaatacccc ggaatgggaa aggaaagttt tcggttatta caaaggaaga
      481 atcagagtga aggatactcc tgatgttcct tcacagaaag agctgattga agatttttcc
      541 aaaaaaccgg gtgaacttcc gtttcctttc ggatacggaa tccttcaggg agtcggaaga
      601 tcaaattttg tatttatgca gaaaaagaaa aataattaat cttactcttt ttcctttgtc
      661 ctgaaaaagc ggatttttga attgagttca tctgcaatct taaaaaggct caatgaattg
      721 tccgctatcg tactcgaatt ctccaggttg gaatgagcaa tttcgtggat cattttgatt
      781 cctttggaaa tttcttcgaa ggctctttca tcatccttta cggtcagttt gatctcttcc
      841 gccttggaca tcacattgcc tgcttctctt tccacattct ctttcatcag cacctgtttt
      901 ccgatcagat cattcatatt cgtcatcaga tccacaattg cgctgacacc ttcaagagtc
      961 cctgaattgt ttttcatcat ttcggacaca ctcagttttc ctttctccac ttctttcctg
     1021 cttctgcgga tgatgacatc aatttctttc agactggctg ctgtttcatc tgccagcttg
     1081 gatatttcat atgccacaac tgcaaatcca aggcccgcct ttccggctct cgccgcttct
     1141 atggatgcat ttaatgcaag caggtttatc ttctccgaga tctcagatat cagtccaaga
     1201 atcttagtca tatcatttga accctgaacg attgaatcca ttcctgaatt gagaacaact
     1261 agagatgctt ctcctgattt agtatcctga taaattcttc cggtgctctg cagagaatat
     1321 ctcacgaccc gttcggtctc tgttatgatt cctgccagct gtcggaagct gcctgcaagt
     1381 gaattcagat catccgactg attttctgca ttccgcgtga ctactgaaac atttttcgaa
     1441 aggctctgaa cggaactgct gatttcctca agtgccgcca cctggctgtg catattgtct
     1501 gagaaatttc tggtggcctt ggacattgta tccgcagaat ttttcagatg tctgctgctc
     1561 tgcgaaacag aatcgatcac tgtgataagc ttgtcattca gtcggttgaa tgtttttccg
     1621 agaagtccga tttcatctga actggtagat tccatttttc tgtaaagtct tccttccgac
     1681 atgtctctcg tgaggctgga aagctgtctt accggccggg tcacgcttaa tgctgctttc
     1741 atgaatactg ctgcggctgt aattacagac aggatgcaga taatcagtgt tccggaggca
     1801 atgatgaaaa taggccggat catgctttcc ctgggaactt cataaacgac attccattct
     1861 gcacttttca gaggaacctt gacaacgtcc atcagtcctc tttcatcggt gtattccaat
     1921 atatctcttt tttgatcatt attcattata attccggcag tttcaggagg ataaaattcc
     1981 gagatattcc tgttcagcat tgcattcacg gaactgatct tgatgactcc ctgtctgtca
     2041 gccagatgaa tcctgtctga aatttctgaa tcgactgcag tgaattcctt tctgacatga
     2101 ctgaagtcca tgacgactcc tgccgctccg agcggattgg aaagatctcc catcactgca
     2161 tttacgaaaa gactcgtcct tccgtccgct gtgtttgtat ctatattcag acttgctttt
     2221 ttcccctgtg aaagagttct gaaaaaccac tgatgcgtgg gagaatttgt cagcttgact
     2281 gccgcatcgt ctttttttcc cgttctgtaa taattcagag
//
LOCUS       DAOQGJ010000480         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30187_length_2319_cov_3.739841, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000480 DAOQGJ010000000
VERSION     DAOQGJ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30187_length_2319_cov_3.739841"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PK683_08530"
     CDS             complement(<1..703)
                     /locus_tag="PK683_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08535.1"
                     /translation="MRREIWYVFVIPAFIANFNCISGMLKKEMKLTPSEYRLLIHDNP
                     KAGDFSVYKSIAKSGLELTYYQQIEKAENGEVIVKKGHSEASGLLSAFKEILFTYKTD
                     VNGYTKEAFITDLNDNVQTRLKTASPEGTDFEMSGKTYNYKYSDYEYLDGSFRLKNDP
                     ENKDYRVRFNTLYKYKKSGIGKIETAQVKLYFTHPNTFFQADRIYTFTVTEAGLPGFS
                     ASPARYILNIVDTDII"
     gene            complement(839..1162)
                     /locus_tag="PK683_08535"
     CDS             complement(839..1162)
                     /locus_tag="PK683_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08536.1"
                     /translation="MKKLILLIILQILFWGCTSTPLSIKTELNKFPVQGDSKRISAEA
                     CGFQFLLLIPIGINDRQVRAYTLLQMQAVGGTLDEIKMEESWIYGLIGTSYCTKFSAD
                     LYGRQ"
     gene            complement(1159..1497)
                     /locus_tag="PK683_08540"
     CDS             complement(1159..1497)
                     /locus_tag="PK683_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08537.1"
                     /translation="MKLNRLIVMLWTALLFIYSCSSGEVLIQKKLTGSEQILGKAKGE
                     GCGSMWIISTAYNFIPIGLNGRIQTAYDNALNSVSGAKGLINISIEETWTWYVLGTAR
                     CTTITGDAVK"
     gene            complement(1768..>2319)
                     /locus_tag="PK683_08545"
     CDS             complement(1768..>2319)
                     /locus_tag="PK683_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08538.1"
                     /translation="VINCVTTSRVSSDKEKEIKSKEHGFITIKFRTTGIENDYGYGPV
                     IDGWRVKFLVDQQEFFSDLFKDGEDYTFPVSAGKHSIKYFFERGRLRPFVGTKIYSSR
                     DENQGMSDSDPRTLNIDIQPDTKHTLIFKQSGSPILFKTMTILYLMPWMWWIYPLIWP
                     AGMGPVFHNQEVILVPEIETSVN"
BASE COUNT          689 a          479 c          388 g          763 t
ORIGIN      
        1 ctatgatatc ggtgtcgaca atattcagaa tatatcttgc cggcgatgca ctaaacccag
       61 gcaatcctgc ttcagtgacg gtaaatgtat aaattcgatc tgcctgaaaa aatgtgtttg
      121 ggtgtgtaaa atacagtttt acctgagcag tttcaatttt tccaattcca gattttttat
      181 atttataaag ggtattgaaa cgtacccgat aatctttgtt ttctggatca ttcttcagcc
      241 gaaacgatcc atccagatat tcataatcgg aatatttata attgtatgtt tttccggaca
      301 tttcaaaatc cgtgccttca ggagaggccg ttttcagcct agtctgcaca ttatcattca
      361 gatctgttat gaaggcttct ttagtgtatc catttacatc tgttttatat gtaaatagaa
      421 tctctttaaa tgcactcagc aggccagaag cctcagaatg acctttcttt acaattactt
      481 cgccattttc cgctttctca atctgctgat aataagttag ctccaaacca cttttagcta
      541 tgctcttata aacggaaaaa tcacctgcct ttggattatc gtgaatcagc agtctgtatt
      601 cactgggcgt cagtttcatt tcttttttca gcattcctga aatacagtta aaatttgcaa
      661 taaatgccgg aattacgaat acataccata tttctcttct catagaaaac tccttttgct
      721 tttcttctct gaagagcagt tttcttttga ttttttttgg ggaaaaatgg aaagcagtgc
      781 cgaattgaag gagtcgggca ctgctttccg aataggctac attttcataa ctgacgagtt
      841 attgtctgcc gtaaaggtca gcagaaaact tagtgcagta agatgtccca atcaatccat
      901 atatccagga ttcctccatt tttatttcat ccaaagttcc accgacagcc tgcatctgaa
      961 gaagagtata tgcacgaacc tggcggtcat tgatccctat cggaataaga agaagaaact
     1021 gaaaaccgca ggcttctgcc gaaattcttt tagagtctcc ctgaaccgga aatttattca
     1081 gttctgtttt gatggaaaga ggcgttgatg tacatcccca gaataaaatt tgcaagataa
     1141 tcaatagaat cagctttttc atttgacagc atctcctgtg attgttgtac accttgcagt
     1201 tcccagcaca taccaagtcc atgtttcttc aatggagata ttaattaacc cttttgctcc
     1261 tgaaacactg ttcaaagcat tgtcgtatgc agtttgaatt ctgccgttca atcctatagg
     1321 gataaaattg tatgcagttg aaataatcca catcgaaccg cagccttccc ctttggcttt
     1381 tcccaatatc tgctccgatc cagtcagttt tttctgaatc agaacttctc cggaactgca
     1441 ggagtatatg aacagcaatg ctgtccataa cataacaata agtctgttta gtttcatttt
     1501 ctactcctga actgaagagg tattttcctc ggattttttc tgaatttttt ttgggagttg
     1561 ttaaaaagta aagattcaaa tagatattgg aaaggcttgc ttacaaaaat cctatctaag
     1621 tcatcagcat agtttattaa ctgatttaga atccaacaac aatgctcgat ctcgaaaccc
     1681 aagcactata cgaatcaaag gaaaaaacac tgggacaagc ctataaaacc attcaacaag
     1741 aaagccctag tgtttatgaa ttaatattca gtttacagaa gtttctattt ccggaacaag
     1801 aattacttcc tgattatgaa aaaccggacc cattccagca ggccagatta aagggtaaat
     1861 ccaccacatc caaggcatca aatataggat tgtcattgtt ttaaataata taggactgcc
     1921 ggactgcttg aatatcaatg tatgttttgt atccggctgt atatctatgt ttaaagtacg
     1981 gggatctgag tctgacatac cttgattttc atctctgctc gaataaattt ttgttcctac
     2041 aaaaggtctg agtctgcctc tctcaaaaaa atatttaata ctatgctttc ctgcagaaac
     2101 gggaaaagta taatcttctc catccttaaa aagatcagaa aaaaattcct gttggtctac
     2161 cagaaatttt actctccatc catctatcac cggaccatag ccgtaatcat tttcaatacc
     2221 tgttgttcta aatttgattg taatgaatcc atgctctttc gatttgatct ccttttcctt
     2281 atctgaactg acccttgatg ttgtcacaca atttatgac
//
LOCUS       DAOQGJ010000481         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30248_length_2316_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000481 DAOQGJ010000000
VERSION     DAOQGJ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30248_length_2316_cov_3.000000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1102)
                     /gene="mltG"
                     /locus_tag="PK683_08550"
     CDS             complement(89..1102)
                     /gene="mltG"
                     /locus_tag="PK683_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNH08539.1"
                     /translation="MKNRGIKIILAGAGAAALLAALVFFTAEHTLGGARGDGSLKTEL
                     VIDSGETSKSVIKELHDSGILKSPGYFRFLLRMTGNSGALKQGVYNLDDGMTARKIMS
                     ILISGKVKTVTFTVPEGYSNRQIGDLLVSKKLASSRQEFLDAASRKEVLEKYKIPADT
                     SEGYLYPSTYTVPFDYKADKIVSMMIRQFQRNIQSVEGASSLDPKDLHQKIILASIVE
                     REAKRNEERPLMAGVFLKRMKIKMPLESCATVQYLFDKPKSRLLEKDLLIESPYNTYL
                     HKGFPPGPISNPGLPAIQASFKPVESDNLFFLLKGDGYHYFSKSHKEHMEAKKKYIDV
                     LYD"
     gene            complement(1102..1662)
                     /locus_tag="PK683_08555"
     CDS             complement(1102..1662)
                     /locus_tag="PK683_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNH08540.1"
                     /translation="MAHIIYQNINDSSIADMVYSENFSLDELKTDENRVFLDCDEDSV
                     LVQTGHPLHSRPLSEIRKMPEFSESDFLIPAVSVDRSGIVLMQAFVNRESLELCLKTG
                     FAHYYSRSRKKLWKKGEDSGHLQKILSIKGPEDCSFFVFLSEQISGACHTGYYSCFYR
                     EYSGKDSFRTVYSEKSFDPEKVYGGA"
     gene            complement(1664..>1844)
                     /locus_tag="PK683_08560"
     CDS             complement(1664..>1844)
                     /locus_tag="PK683_08560"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH08541.1"
                     /translation="GVERLIEIIQKNISLPSEELIDILLDSVREFRKEGELDDDASLI
                     CLTRKDRVTEVGMRG"
     assembly_gap    1845..1854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1855..2288)
                     /locus_tag="PK683_08565"
     CDS             complement(<1855..2288)
                     /locus_tag="PK683_08565"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH08542.1"
                     /translation="MDFIQSEDGRLYIFLGDITGHGVGSGLLVSAVKAIIRSNVEMNR
                     PLQKIFETINRFLNERYPGFEFMSLFGLVYDSQSSSSEYINAGHVPPVIIGKNGDRRR
                     FEATETLLGVAPGRYPSVRLSLEPGDKMFVFSDGIVEALNPA"
BASE COUNT          589 a          561 c          472 g          684 t
ORIGIN      
        1 tgcccaagtt cagtcagcac atgttcaagc tgctgaccga gaaattcgat ctgcccgtct
       61 atttttttga gttcttcgtt ctcttccatc agtcgtacag tacgtcaatg tatttctttt
      121 tagcttccat atgctctttg tgggatttgg aaaaataatg atagccgtcc cctttcagaa
      181 gaaagaaaag attgtccgat tccaccggtt tgaatgaggc ctggattgca ggaagtccag
      241 gattggaaat cggcccaggt ggaaaccctt tgtgcagata tgtattgtaa ggagactcaa
      301 tcagaagatc cttttccaga agtctgctct tcggtttatc aaaaagatat tgaacagtag
      361 cacagctctc cagaggcatt tttatcttca ttcttttcag aaagactcct gccatgagag
      421 gacgttcttc atttcgtttg gcttctcttt ccacgatgga tgcaagtatg attttctgat
      481 gaagatcctt cggatcaaga gaggatgccc cttccacaga ctggatgttt ctctgaaact
      541 gtctgatcat catggaaacg attttgtccg ccttgtagtc gaagggaaca gtataagtgc
      601 tcgggtacag ataaccttcc gatgtatctg caggaatttt atatttttcc agcacctcct
      661 ttctggaagc cgcatcgaga aattcctgtc tggacgaagc aagttttttt gagacaagca
      721 ggtctcctat ctgacggttg ctgtatcctt ccggtacggt aaatgtaact gtctttacct
      781 tgccggaaat cagaatgctc ataatcttgc gcgcagtcat tccgtcgtcc agattgtaga
      841 ccccctgctt cagagcaccg gagtttcccg tcattctgag aaggaatcga aaataacctg
      901 gagacttgag gattcctgaa tcatgaagtt cctttatgac cgatttggat gtttcaccgg
      961 aatcaatgac tagctcagtc ttgagactgc cgtcaccgcg ggctcccccc agagtatgct
     1021 ctgcagtaaa gaagaccagt gcagcaagaa gtgcagcagc tcctgctcct gctaatataa
     1081 tttttattcc tctatttttc attatgctcc cccgtaaact ttttccggat caaaactctt
     1141 ttctgaatac acagtcctga aagagtcttt tccggagtat tccctgtaga aacaggaata
     1201 atatccggta tgacatgcac ctgaaatctg ttccgacagg aatacaaaaa agctgcagtc
     1261 ctcaggaccc tttatagaaa gaattttttg aaggtgtccg ctgtcttcac cttttttcca
     1321 gagtttcttc ctgcttctgc tgtaataatg agcaaagccg gttttcagac agagctcaag
     1381 gctctccctg ttcacgaatg cctgcatcag aacaatgccg gatctgtcta ctgagactgc
     1441 agggattaga aaatcggatt cagaaaactc cggcattttt cgaatttcag acagaggccg
     1501 ggaatgcagg ggatgtcctg tctgcacaag cacagagtcc tcatcacagt ctagaaaaac
     1561 cctgttttca tccgtcttca gttcatccag actgaagttt tccgaatata ccatatctgc
     1621 aattgaactg tcattaatgt tctgataaat gatatgtgcc aaattaccct ctcatcccaa
     1681 cttcagtcac tctgtccttt cttgtcaaac agataagaga ggcatcatca tcaagttctc
     1741 cttcctttct gaactctctg acactgtcca gaagtatatc gatcagctct tcactcggaa
     1801 gggatatatt tttctgtatt atctcgatca gtctttccac tccgnnnnnn nnnnaaggca
     1861 ggattgaggg cttccactat tccgtccgaa aaaacgaaca ttttatcacc cggctcaaga
     1921 gaaagtctca ccgaggggta gcgccccgga gccactccga gaagggtttc ggtcgcttca
     1981 aatcttctgc ggtccccgtt ttttcctatg atcaccgggg gcacatgtcc tgcattgatg
     2041 tattctgaac tgctgctctg tgaatcatat acaagtccga aaagactcat aaattcaaat
     2101 ccgggatatc tctcattcag aaatctgttg atcgtttcaa atattttctg aagaggtctg
     2161 ttcatttcaa cattcgaccg gatgatggcc ttcactgcac tcacgagcag tccgcttcct
     2221 accccgtgtc ctgtaatgtc tcccaggaaa atatacagcc tgccgtcttc agactgaatg
     2281 aaatccacgt agtcccctga tattccggat gctgca
//
LOCUS       DAOQGJ010000482         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30272_length_2315_cov_3.519912, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000482 DAOQGJ010000000
VERSION     DAOQGJ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30272_length_2315_cov_3.519912"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PK683_08570"
     CDS             complement(<1..479)
                     /locus_tag="PK683_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HNH08543.1"
                     /translation="MMEDKELSKVEYIKAESKGLRGTLAEELKDGNDFFSEDNTQLLK
                     FHGLYQQKSRERIKDEKGEDIEQPHSFMLRGRIPGGRMNAAQYLIWDDLGDRFGGGAL
                     RLTTRQSIQFHALPKTSLQTVITEINRINQSTMGACGDVVRNVTQAVNPWKRKDLTQL
                     "
     gene            667..2076
                     /locus_tag="PK683_08575"
     CDS             667..2076
                     /locus_tag="PK683_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNH08544.1"
                     /translation="MSDIRYICISDLHFGAYNSLLTGTDEKGFSDHSSDSPVLEHLLR
                     IIENVVGSVNRKKRARFILNGDIFELALANTNEAVMSFDSYLRLCFTKTRKEIFSDKL
                     IFVPGNHDHHLWETARERQYLDYMTKFEPRDFISPPWHHTRMLKPKPLKSRFISTVMK
                     RHKLLKSGRALTVYPNLCLVNQDLKKYTVITHGHFIESMYFLMSHLSSVLLNSESFPE
                     TTDDIEKENFAWIDFFWSMLGRSGNAGLRVGVMYDMLQDEKGIQRLASNLINYVMKTE
                     KTSVSIQDFLKPAINFLLTKIITGIAVNERGLTDDLLGKGALEGMYSYINGPLRLQFL
                     EENNSVLPNHMDFIFGHTHKPFCRFYSGFSDSGYPDQIAVYNTGGWVADTVIPQKQVG
                     GAVSFIDEDLNIVLMRLYNENNFEIRFESCNGEDNPLLEKLKAGIDLKSSLYYNFSRA
                     VKEEIEKKRRIILSRIKQD"
BASE COUNT          651 a          467 c          499 g          698 t
ORIGIN      
        1 agctgtgtca ggtcttttct tttccagggg ttgactgcct gtgttacatt tcggacaaca
       61 tctccgcagg cccccattgt actctggttg attctgttga tttcagtgat gactgtctgg
      121 agtgatgttt ttggaagtgc atgaaactgg atggactgtc ttgtggtaag ccggagtgcg
      181 ccgcccccga atcggtcacc cagatcatcc cagatcagat actgagctgc attcatcctg
      241 ccgcctggga tacgacctct gagcataaaa gaatggggct gttctatgtc ttcccctttt
      301 tcatccttga tccgttccct ggatttctgc tggtacaagc cgtgaaattt cagaagctgc
      361 gtattgtctt ccgaaaagaa gtcatttccg tctttcagtt cctctgccaa ggttcctctt
      421 agacctttgg attctgcctt gatatactct actttgctca gttctttgtc ttccataatg
      481 ccattcgccc gcattgagaa agactgtcaa ttagattaga aaaaattcaa tatattggag
      541 gatttgtttc gcactcctga tcggaatgtc ctgattttta attcggagat ttagattcat
      601 tgaactttta gaactcaata tgaatatgga gaaattgaaa atcgaaggaa gtcggtcaat
      661 aaatgaatgt ctgatatcag gtacatctgt atttccgatc tgcatttcgg tgcatacaac
      721 agtcttctga caggcacaga tgaaaaaggg ttttccgatc acagttcaga cagccctgtg
      781 cttgaacatc ttctgagaat tatcgagaat gttgtaggct cagtgaacag aaagaaaaga
      841 gcccgtttca ttctgaacgg agatattttt gaactggctc tggcaaatac aaatgaggca
      901 gtgatgtcct ttgattccta tctgcgcctc tgctttacta aaacgaggaa agaaattttt
      961 tcagacaagc ttatctttgt accgggcaat catgatcatc atctttggga gacagcccga
     1021 gaacgtcagt atctggacta tatgacaaaa tttgaaccaa gggattttat aagtcctccc
     1081 tggcatcata ccagaatgct caaacccaaa cctctcaagt cacgttttat ttccactgtg
     1141 atgaaaagac ataagcttct gaagtccgga agagcattga cagtatatcc caatctttgt
     1201 ctggtcaatc aggatctgaa aaagtatact gtgatcactc atggtcattt tatagagtct
     1261 atgtattttc ttatgagtca tcttagctca gtccttctga attcagaatc ttttcctgaa
     1321 acaacggatg atatcgaaaa agagaatttt gcatggatag attttttctg gtccatgctt
     1381 ggaagatcgg gaaatgcagg acttagagta ggagtgatgt atgatatgct tcaggatgaa
     1441 aaaggaattc agagacttgc ttccaatctg atcaactatg tgatgaaaac agaaaaaacc
     1501 tctgtttcca ttcaggattt tttaaaacct gcaataaact ttttactcac aaaaatcatt
     1561 acaggaattg ctgtcaatga aagagggctg acggatgacc tgcttggcaa gggcgctctg
     1621 gaggggatgt attcctatat aaacggtcct ctgcggcttc agtttctgga agaaaataat
     1681 tccgttctgc cgaatcatat ggacttcatt ttcggtcata cccacaagcc cttctgccgt
     1741 ttttactcag gattttccga ttcaggatat ccggatcaga tcgcagtata caatacagga
     1801 ggatgggtcg ccgacacagt cattcctcag aagcaggtgg gcggtgcagt gtccttcatt
     1861 gatgaggatc tgaatattgt gctgatgaga ctgtacaatg agaacaattt tgaaatcaga
     1921 tttgaatcct gcaatggaga ggacaaccct cttcttgaaa aacttaaagc ggggattgat
     1981 cttaaatcat cattatatta taatttcagc agggcagtca aggaagagat tgaaaagaaa
     2041 agaagaatca ttctgtccag gataaagcag gactgacaca gttttcggat tgtcgaggtc
     2101 ttaatccact gcagattcac aaacattcaa ctcacctgca cgaaatgcgt gagatgagtt
     2161 tgaatgatct gactttcaag gaagattgaa ttttatattc aaagaatcta atgttgtttt
     2221 ctgcatatca tcccaggtac ctgctccggc tgttccaatt ttgctgaaaa gcgtgtccac
     2281 tctcgatgta gagggataat cggaagatac tgccg
//
LOCUS       DAOQGJ010000483         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30328_length_2313_cov_3.161205, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000483 DAOQGJ010000000
VERSION     DAOQGJ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30328_length_2313_cov_3.161205"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /gene="mrdA"
                     /locus_tag="PK683_08580"
     CDS             <1..1894
                     /gene="mrdA"
                     /locus_tag="PK683_08580"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004445747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNH08545.1"
                     /translation="FQLFNLQVVNGSENTLKAERFVHKSEVLPASRGQVYDRNFRSPE
                     RSFALIQNSASLDVIVNTNLFKNNPEKIKGFVTNFYTALSIPKIYFENELTEPKFTKK
                     IKSKTPIVLLQGITNAQIERITQFDNIGRYVVFVPTPKRIYTMGPALAHVTGYVGLPN
                     TNDLKNPEIKSYQLIGKTGLELQYDKYLRGVDGNRYQKRNSEGNIEEENIISHAQMGN
                     NVILTIDKDVQKAAFNALNGFRGTVIAMKVATGEILAMASNPSYDPNIVSGKNSQTRQ
                     AHLKKVNKNGGFLNKAIMSKFPPASTYKTLVGLAALESEHKIRYSASQTFHCNGRYYL
                     PSSLASVPDQEFMCWDKKGHGTNDLPHALEKSCSVYFYNLGHKLGSEAIIYYSRLFGL
                     DKKSNIDLPSEIEGFVPSNEWKKRSYGQKWYDGDTINLSIGQGFISSTPIGMALFYMA
                     IANNGKVHQPHLVSEIRNPWDNSLIYKTVPKVIRDIPLKASTIEAIREGLRLVGKTGT
                     ASRVLNQPDVPEIAGKTGTAQTRRRGQSSSNHAWFIGYAPYNAPVSEQVLVTVFVEYG
                     VGGSIGAGPIARDVFKAAFPPGTFVKKDGKTDGGRMSEEIPLPNETNLPEEETDDTGS
                     DEDTIQ"
     gene            1891..>2313
                     /locus_tag="PK683_08585"
     CDS             1891..>2313
                     /locus_tag="PK683_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016757024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNH08546.1"
                     /translation="MKDRRFIERTLDWFLIFCVMIVILCGVVTLYAQEVNIAEGAPGR
                     WYKQLLYAGIGFVGLLFMTRINYQLLGSYALLIYLVAIVLLGLTLIPGIGYLPSGRGA
                     RSWLKLGPFSIQTSEFAKLSTVILLGQYLVIKEREMRNI"
BASE COUNT          696 a          478 c          546 g          593 t
ORIGIN      
        1 atttcagctt tttaatcttc aggttgtcaa cggaagcgag aatactctga aggcagagag
       61 atttgttcat aagagtgagg ttctgcctgc ttcgagagga caggtgtatg acagaaattt
      121 cagatctcct gaaagatcgt ttgcgctgat acagaactct gcctcgctgg atgtgattgt
      181 aaatacaaat ctgttcaaga acaatcctga aaaaatcaaa ggattcgtga ctaattttta
      241 tactgcactt tccattccca aaatctattt tgaaaatgaa ctgactgagc ctaaattcac
      301 aaaaaagatc aaatccaaaa ctcccatagt ccttcttcag ggaattacga atgctcagat
      361 tgaaagaatt actcagtttg acaatatcgg cagatacgtt gtgttcgtgc cgactccgaa
      421 aagaatctat acaatggggc ctgcacttgc gcatgtcacc gggtatgtgg gacttccgaa
      481 tacaaatgat ctgaaaaatc cggaaatcaa atcctatcag ctgatcggga aaaccggtct
      541 tgagcttcag tatgacaagt atctgagagg agtggacgga aacagatatc agaaaagaaa
      601 ctctgaagga aacatagaag aagaaaatat catcagtcat gcccagatgg gcaacaatgt
      661 tattcttaca attgacaagg atgttcagaa ggcagcattc aatgcactga acggatttcg
      721 cggaacagtc attgcaatga aagtggcaac gggtgaaatt ctggcaatgg catccaatcc
      781 aagttatgat ccgaatattg tatcaggaaa gaattctcag acaagacagg ctcatcttaa
      841 aaaggtaaac aagaacggcg gatttctgaa caaggcaatc atgtccaagt ttcctccggc
      901 ttccacatac aaaacactgg tgggacttgc cgcactggaa agcgaacata agattcgtta
      961 ttctgcatcc cagacatttc actgcaacgg aagatattat cttccttctt ctcttgccag
     1021 tgtgcctgat caggaattta tgtgctggga taaaaaaggt catggaacaa acgatcttcc
     1081 tcacgccctg gagaaatcct gttcagtgta tttttacaat ctgggtcata aacttggatc
     1141 tgaagcgatc atctattatt cccgtctgtt cggtctggac aaaaagtcca atatagatct
     1201 ccctagtgag atcgaaggtt ttgttccaag caatgaatgg aagaaaagat catacgggca
     1261 gaaatggtat gacggagata caatcaatct ttccatcggt cagggattca tttcctccac
     1321 tccgatagga atggcattat tttatatggc gattgccaat aacgggaaag tgcatcagcc
     1381 ccatctggtc agtgagatca gaaatccctg ggacaacagt ctgatctaca agacagttcc
     1441 taaggtcatc cgggacatcc ctctgaaggc ttctacaatc gaagcaatca gagaaggtct
     1501 gaggcttgta ggaaagacag gtactgcctc cagagttttg aatcagcctg acgttccgga
     1561 aattgcaggc aagacaggta ctgcacagac aagaagaaga ggacagtcca gttccaacca
     1621 cgcgtggttt atcggttatg ctccctacaa tgcccccgtt tcagaacagg tcttagtgac
     1681 agtgtttgtg gaatacggag tgggtggatc gatcggggca ggtcctatcg caagagatgt
     1741 attcaaggca gcgttcccgc ctggaacctt tgtgaaaaag gacggaaaga ccgacggcgg
     1801 cagaatgagt gaagagattc ctctcccgaa tgaaaccaat cttcctgaag aggaaacaga
     1861 cgatacaggc tcagacgagg atacaattca gtgaaggaca gaagatttat agaaagaacg
     1921 ctggactggt tcctgatttt ctgcgtgatg attgtcattc tctgcggagt agtcactctg
     1981 tatgcacagg aagtcaatat tgccgagggt gctccgggca gatggtacaa acagttatta
     2041 tatgccggta tagggttcgt cggacttctg tttatgacta gaatcaatta ccagcttttg
     2101 ggatcgtatg cacttcttat ttatcttgtt gccattgttc ttcttggact gacgctgatt
     2161 cctggaatcg gatatctccc gtcaggaagg ggagccaggt cctggctgaa gcttggaccg
     2221 ttcagcatac agacgagcga gtttgcgaaa ctttccacag tgattctgct tgggcagtat
     2281 cttgtcatca aggaaaggga gatgagaaac att
//
LOCUS       DAOQGJ010000484         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30334_length_2313_cov_2.779894, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000484 DAOQGJ010000000
VERSION     DAOQGJ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30334_length_2313_cov_2.779894"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="PK683_08590"
     CDS             complement(<1..1658)
                     /locus_tag="PK683_08590"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH08547.1"
                     /translation="MTIRQKLFSILGLSQLIIISALGISFYILIDAVKDEPQNNRAIE
                     LSNAFKKELHDREEFLKIILKETVSNPVRLEFLNAGLLNREIFKQDSAYYNKIKKEYK
                     IDIMEIGDQAGKVVYRYHRPNDFGDDKSHQKIIQEALAGKITSTLELGHSGLGLRATG
                     PIYRSGTVLLGQKVDRDFMAGITGRWQVKLAVMENNKILASSDDAVMQFLQERKNLNS
                     MNSGERVKFEGRHFYLIKIPYESAGLSNRNLSFAVMIDETEVHTASAKIWRIFGIAAL
                     LFFSSVFYVSYLFSKNIINAIRQLNSAMSNLDDEKFQPNVDTFRKDELGEIGKVFLSM
                     KDEILNYQKHLEKLVDEKTIELKNSLEEIQKLKEHQDGDYFLTSLLLKPLNQSDTVYE
                     NINIDTIIRQKKRFTFRNREAEIGGDLCAADLISLEGRDFAAFINADAMGKSIQGAGG
                     ALVVGTVFKSFITRTKEEKNYSEKPPEIWLRDCHSELQKVFSSFDGSMILSAVIGLVD
                     NLTGTLYCINAEHPWPVLYRDGQAQFLFQDTSSRKIGLSFYMDF"
     gene            complement(1815..2021)
                     /locus_tag="PK683_08595"
     CDS             complement(1815..2021)
                     /locus_tag="PK683_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08548.1"
                     /translation="MKLTAAILLSLSFAAVSVSAKQPYYQCVGKLKGKEVKFAGYYRD
                     AATAEKQFSSQRKGTTEITCTEKK"
BASE COUNT          608 a          496 c          448 g          761 t
ORIGIN      
        1 tcaaagtcca tataaaagga aagccctatc tttctcgaag acgtgtcctg aaaaagaaac
       61 tgtgcctgtc cgtctctgta aagcacaggc caaggatgtt ctgcgttgat gcagtaaagg
      121 gttcctgtca gattgtccac aagtccgatc actgcagaaa gaatcatgct tccgtcaaaa
      181 ctggaaaaca ctttctgaag ttctgaatga cagtctcgaa gccagatttc aggaggtttt
      241 tctgaatagt ttttttcttc cttagttctt gtgatgaagg atttgaatac agttcctaca
      301 actagtgccc cgcctgcacc ctgtatggat tttcccatcg catctgcatt gatgaatgcg
      361 gcaaaatccc tgccttccag agaaataaga tctgccgcac agagatctcc tccaatttct
      421 gcttccctgt ttctgaatgt gaatctcttt ttctgtctga tgattgtatc aatatttatg
      481 ttttcatata ctgtatcaga ctgattcagc ggtttcagca ggagggatgt gagaaaatag
      541 tctccgtcct gatgctcttt gagtttctga atttcttcca gactgttctt cagttcgatt
      601 gttttttcat cgacgagctt ttcgagatgc ttctggtagt tcagaatctc atctttcatc
      661 gacagaaata cttttccaat ttctcccagt tcatcctttc tgaaggtatc cacattaggc
      721 tgaaattttt catcatccag attgctcatt gctgaattga gctgtctgat tgcatttata
      781 atgttttttg aaaagagata ggatacataa aaaacggaac tgaaaaacag aagggctgca
      841 atgccgaata tcctccatat cttcgcggaa gccgtatgaa cctcagtctc gtcaatcatc
      901 actgcaaatg acagatttct gtttgagaga cctgcagact cgtacggaat tttaatgaga
      961 taaaaatgtc ttccttcaaa tttgactctt tcgccggaat tcatactgtt caggtttttc
     1021 ctttcctgaa gaaactgcat cactgcatca tcgcttgaag caaggatctt gttattttcc
     1081 ataactgcaa gtttcacctg ccatctgcct gtgatccctg ccataaaatc tctgtctact
     1141 ttctgtccta aaagtacagt gccgcttctg taaatcggtc ctgttgccct gagtccaaga
     1201 ccgctgtgac ccagttcaag agtggatgtg attttcccgg ccagagcctc ctgaatgatc
     1261 ttctgatgac ttttgtcatc tccgaaatca ttcggcctgt gatagcggta aacaaccttc
     1321 cctgcctgat cgccgatttc cataatgtct attttatatt cttttttaat tttattataa
     1381 tatgctgaat cctgcttgaa tatttccctg ttgagaagtc ctgcattcag aaattccaga
     1441 cggaccgggt tcgatactgt ttccttcaga ataattttca ggaattcctc cctgtcatgc
     1501 agttcctttt tgaaagcatt tgacagttca attgctctgt tgttctgagg ttcgtctttt
     1561 actgcatcaa tcagtatata aaatgagatc ccaagcgctg atataatgat cagctggctc
     1621 aggccgagaa ttgaaaacag tttctgtctg atagtcatag attttatcct gaatccatta
     1681 tcagcactat tgcagcagta tgattacagt cctgtgaaat aagaaacagt ataaatttac
     1741 gattcagtta caaaagggat ttatcggatg agagaggagt atggccctct gcagagagaa
     1801 ggccttttac agaattattt tttttcagtg caggtaattt cagtagtgcc tttgcgctga
     1861 gatgaaaact gcttttcagc cgttgctgca tctctgtaat atcctgcaaa ttttacttct
     1921 tttcctttca gttttcctac acattggtag taaggctgtt ttgctgatac ggacactgcc
     1981 gcaaaagaaa gggataaaag aatcgctgct gtaagtttca taaactctcc tgaatgttta
     2041 ttttttagac tgaagacttt ataaaaagtt cgattttcgt tctcactgaa ctgaatttgc
     2101 cggcagacaa aactcttgac tgacctctct ccacaattga aactgcctat tatgaagaga
     2161 ctgcctttgg gaattcagac ctttgcggat atgattgagg aaaactgtta ctatgtggat
     2221 aagacagagt ttatccgcag actggctgat ataggcagat acttttttct gagccgtccc
     2281 cgccgattcg ggaaaagttc ttttctggac aca
//
LOCUS       DAOQGJ010000485         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30368_length_2312_cov_2.715552, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000485 DAOQGJ010000000
VERSION     DAOQGJ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30368_length_2312_cov_2.715552"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..623)
                     /locus_tag="PK683_08600"
     CDS             complement(66..623)
                     /locus_tag="PK683_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH08549.1"
                     /translation="MLQKIFSALAFAADKHRGQKRKDTLGSPYINHPIEVAERLVHFG
                     VEDPNVLQAAVLHDTVEDTEATEEDILVMFGNKVMSIVMELTDDKSLSKKERKQRLIE
                     KSEKFSLEAKLVKLADHTCNLHDIAVSTPRDWSDERCMEYAFWSSEVIKNCRSVNEGL
                     EKEYDRVHGLVLKKFSGINLHLTED"
     gene            complement(638..1891)
                     /locus_tag="PK683_08605"
     CDS             complement(638..1891)
                     /locus_tag="PK683_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNH08550.1"
                     /translation="MANESFIQPNHIYPFMWFMDLSLKGLLKAVHNIEDVIVSEKDRE
                     MLKSLQKERVIFISNHPSTKEPPISYLIGNLMYSRFYYMASREVFDWANGAVGKVIQS
                     IGAFSVIAGSADRESLKMTRSILAKPEGKLVLFPEGEPTGGENDNLLPFQPGVTQLGF
                     WGYEDAVKTDPKADILILPAFIKYRFSESTENIRKDIDSSLSNMERRLGIGKAGKTLV
                     HRLFSIGKRLMEKNEIEYGIRPDDNQSYDYRIGRLRHTILDDVAEKLSLKKYNKDANA
                     IDKLRYVLSTLELVSIGAPDPKNELPPADKAKWGRKVCQKVYDFITIQTAYLDFASAE
                     RVYEWIYRFENEMFGSSNPRPSKAYISFAPAKHLAEYYTQYKSAKSKKEVVESLTHEM
                     KESIQKLLDEEVSKSFPIFPAEFKF"
     gene            1952..>2312
                     /locus_tag="PK683_08610"
     CDS             1952..>2312
                     /locus_tag="PK683_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNH08551.1"
                     /translation="MALMEEFDRQGSFLFRWRSYVPSVILIISAFFVPGYKYAGGNYS
                     SHLYWSIPALIVSLLGLFVRCYTIGYAPEKTSGRNTKKQVAETVNQSGIYSMVRHPLY
                     LGNFLMFFGIVLFIKSIA"
BASE COUNT          563 a          509 c          471 g          769 t
ORIGIN      
        1 cagtgtctta tttccgcagg cggggcagaa gcagggggta cccggttttc cgtgcattga
       61 aaaattcagt cttccgtcag atgaagattg attcctgaaa actttttcag aacaagaccg
      121 tgaaccctgt catattcctt ttccagtcct tcatttacag atctgcagtt tttgatgact
      181 tccgacgacc agaaagcata ttccatgcat ctttcatcac tccagtcccg aggagtgctc
      241 actgcaatat catgaagatt gcatgtatgg tcggccagtt tgacaagctt agcttccagc
      301 gagaactttt ccgacttttc aatcagtctc tgctttcttt cttttttgga aagagacttg
      361 tcatccgtaa gttccattac gatggacata accttgtttc cgaacatgac aagaatgtct
      421 tcctcagtcg cttccgtgtc ttcgactgta tcatgaagta ctgcagcctg aagaacattc
      481 gggtcttcga cgccgaaatg aaccagtctc tccgccactt caatcggatg attgatatag
      541 ggagatccca gagtatcctt tctcttctgc cctctgtgct tgtccgcagc aaaggcaagt
      601 gctgaaaata ttttttggag cacagtcgca ttcatcttca gaatttaaac tccgcaggaa
      661 atatagggaa tgatttgctc acttcttcat ccagaagctt ctgtatgctt tctttcattt
      721 catgagtcag actttctacg acttcttttt tgctctttgc agatttgtac tgagtgtagt
      781 attctgcaag atgttttgcc ggtgcaaaac tgatataggc tttggaaggt ctaggattcg
      841 atgacccgaa catttcattt tcaaatcggt atatccattc atacactctt tcggcagatg
      901 caaaatccag atacgctgtc tgaattgtaa tgaaatcata tactttctgg catacttttc
      961 ttccccattt tgccttgtct gcgggaggaa gttcattttt tggatcgggt gcacctatgg
     1021 aaaccagttc aagagtggac aggacatacc gaagcttgtc gattgcattt gcatccttat
     1081 tgtatttttt cagactgagt ttttctgcta catcatccag gatcgtgtgc ctcagtctgc
     1141 cgatgcggta gtcataactc tgattgtcat cgggtctgat tccgtattct atttcatttt
     1201 tctccataag ccttttgcct attgagaaaa gtctgtgcac cagagtcttt cctgcctttc
     1261 ctatcccgag tctcctttcc atattggaaa gggaactgtc aatgtcctta cggatgtttt
     1321 ccgtgctttc actgaacctg tatttaatga atgcgggaag aatcagtatg tctgccttag
     1381 gatctgtctt gactgcatct tcgtatcccc agaacccaag ctgtgtcacg ccgggctgaa
     1441 atggaagaag attgtcattt tctcctccag tcggttcacc ttcgggaaaa agaaccagtt
     1501 tgccttccgg ttttgcaagt attgaccttg tcattttcag cgactctctg tcagcacttc
     1561 ctgcaattac ggaaaatgcc ccaatggact gaatgacctt tcctactgct ccgtttgccc
     1621 agtcgaaaac ttctctggat gccatataat agaatctgct gtacataagg tttccaatca
     1681 ggtaactgat gggaggttct tttgtggacg gatgattgga tatgaaaatc accctttctt
     1741 tctgaagtga tttcagcatt tccctgtctt tttctgaaac aattacgtct tcaatgttgt
     1801 gaactgcctt gaggagccct ttcagactca gatccataaa ccacataaac ggatatatat
     1861 ggttcggttg tataaaagat tcattcgcca tattttaata attccctaat tgaggcagtt
     1921 tatacaacta gtttttttag ggagttttac aatggcttta atggaagaat ttgacaggca
     1981 gggaagtttc ttattcaggt ggagaagtta tgtaccttct gtaatactca tcatttccgc
     2041 attttttgtt ccaggttata aatatgcagg cgggaactat tcctctcatc tttactggtc
     2101 aattcctgca ctgattgtca gtctgctggg tctttttgta agatgttata caatcgggta
     2161 tgctcctgaa aaaacttccg gcagaaatac gaaaaagcag gtagcggaga ctgtcaatca
     2221 gtctggtatt tattccatgg tgagacatcc tctgtatctg gggaactttc tgatgttttt
     2281 cgggatagtc ctgttcataa aaagcatagc tt
//
LOCUS       DAOQGJ010000486         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30445_length_2308_cov_3.367510, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000486 DAOQGJ010000000
VERSION     DAOQGJ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30445_length_2308_cov_3.367510"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            831..>2308
                     /locus_tag="PK683_08615"
     CDS             831..>2308
                     /locus_tag="PK683_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH08552.1"
                     /translation="MTKKFALFSLSNILFAVFLFFMLTVTSAAFWRVCAFRDMHKKIT
                     EHFTVSGSDQGLNENISSALSAEYFFLFLNLGVFILLPFGWTKVRRKLKEEQNARTEL
                     SRYKSVLDSLSCSVIAVDEDQKIIYTNGALRKLLSEAEIDISRLKAGSPLSDAGLEIL
                     GRNFIPQKQFFRNSTDEKSVSVEIGPRNFRSDSSPVILEDGSRLGFAAVLSDLKRERE
                     FTAEKKQLYAEIENYKAQINAVYRAQALIEFEMDGTIITANDIFLNIMGYTLDEIKGR
                     HHSIFAEPAYVQSQEYRQFWTGLNQGKFQSAEFRRIGKGGREVWLQAVYSPVLDRDGK
                     PLKVIKFASDVTVQKLKYADYEGQISAVGKSQAVVEFKMDGTVITANEIFLSVMNYTL
                     EEIKGHHHGMFAEPAYVQTEEYRQFWDALRRGEFRSSEIKRLGRDGKEVWLQAVYSPI
                     FDLNGRPFKVVKYASDITPQKLRNADYEGQIAAIRKSQAVIE"
BASE COUNT          692 a          449 c          530 g          637 t
ORIGIN      
        1 atactgcggc tgtaagtgcc gcagtgctta tttctgaaca gcacctgatg aaaaatgaaa
       61 aaacaatgta aaatattaca tctgacttgt tttcccggga tattaaaacg atagcgaagc
      121 gtctgaagct gccgccaaaa tctgaagctg ctgccaaaat ctgtaaaagc ctcggttacc
      181 ctgcctgccc gcattcagta ataaggtaaa tccgctttta ggcaatgaat aactcacctt
      241 acacaaatac cgtatactgt ccaaacgcag taatgttcag aaaacagtag gaaaaataga
      301 tcagtaaaaa cagcggtttt ctagtttgca gcagtttcca agtttgcagc aacttccaag
      361 tttgcgagcc ctcaagctgt cggcaggtgt tccagtcatg cagccgtcgt aggcgtaagg
      421 tgaatgaatt gtatacaatt tcattagaaa ttcagataag ctcaagtatc tcctttttct
      481 gaacattgat ataatcactc agcttacaga aatatgatat actgtacatc aggaaaatcc
      541 ggcagtttaa catcttgcaa tgaatcagta aaaatagcgg tttccattct tgcacggttt
      601 tttttagtca tcgaaaattc agccaaaggc ataaggaaag cagtcagcag tacggaatat
      661 tcattatttt cgagggattt tgtctcagca gaaatgattt tttcaataca cttcattttc
      721 ttgacattag gtttcctctc tgtacagttg taatgccgat acagtttaaa tgtgctcagg
      781 gtgtccgctg aagttcagtt cgctctgcag ttgcagcttg gcagaggtct atgacaaaaa
      841 aattcgcttt attcagtcta tcaaacattc tgttcgcagt ttttcttttc tttatgctta
      901 ctgtcacttc tgcagcattt tggcgtgtct gtgcattcag ggatatgcat aaaaaaatca
      961 ctgaacattt tacagtttcc ggttcagatc aggggttgaa tgagaatatt tcatcagcac
     1021 tgagcgcaga atactttttt cttttcctga acctgggagt ctttattctg cttcctttcg
     1081 gctggacgaa agtcagaaga aaactgaaag aagagcagaa tgcaagaact gaattgtcca
     1141 gatacaaatc tgttctcgac agtctttcct gcagtgttat tgcagtggat gaagatcaga
     1201 aaatcattta tacaaacggg gctttgagaa aacttttatc ggaagccgaa atcgacattt
     1261 ccagactgaa ggcaggcagt cccctgtccg acgcagggtt ggaaattctg ggcagaaatt
     1321 ttatccctca gaagcagttc ttcagaaatt caactgatga aaaatccgtt tcagtggaaa
     1381 tcggtcctcg gaattttcgt tctgattcaa gtcccgtcat cctggaagac gggagcagat
     1441 taggctttgc ggcagtgctg tctgatctga aaagggaacg tgagttcact gcggaaaaaa
     1501 agcagttata tgccgagatc gaaaattaca aggctcagat caatgccgta taccgggctc
     1561 aggcactgat tgaatttgaa atggatggga caatcatcac cgcaaatgac attttcttaa
     1621 atattatggg atacaccttg gatgaaatca agggaagaca ccacagcata tttgcagagc
     1681 ctgcatatgt gcagagtcag gaatacagac agttctggac gggactgaat cagggaaagt
     1741 ttcagtctgc tgaattcaga agaattggaa aaggcggcag agaggtttgg cttcaggcag
     1801 tctacagtcc tgtcctggac agagatggaa aacctcttaa ggttatcaag tttgcatcgg
     1861 atgtcactgt gcagaagctg aaatatgcag attatgaagg gcagatctca gctgtcggca
     1921 agtcacaggc ggttgttgag tttaaaatgg acgggacagt catcactgca aatgaaatat
     1981 ttctttcagt aatgaactat actttggagg aaatcaaggg gcatcaccac ggtatgtttg
     2041 cagaacctgc atatgtgcag acggaggaat acagacagtt ctgggatgcc ttgagaagag
     2101 gagagttcag gtcttcagaa attaagaggc ttggcaggga tggaaaggaa gtctggcttc
     2161 aggcagtcta cagtccgatt tttgatctga acggcagacc tttcaaagtt gtaaagtatg
     2221 cctcagacat cactcctcag aagctgagaa atgcagatta tgaagggcag attgcagcaa
     2281 tccgtaagtc ccaggcagtc attgagtt
//
LOCUS       DAOQGJ010000487         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30447_length_2308_cov_3.173990, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000487 DAOQGJ010000000
VERSION     DAOQGJ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30447_length_2308_cov_3.173990"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PK683_08620"
     CDS             <1..690
                     /locus_tag="PK683_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08553.1"
                     /translation="MIRSYSEGTGPTDGVWKLGGMWINDKAGNGKYYSSTDLNKNFTL
                     YSASTTQTADFYPPELKSITIDKTDVNFDQTFTVSADVSDISTETTSGDQNAASTSAS
                     TGTSTSTATTTLSSTVASGVKSVKVVLYSPLKLLDDSLGVSKSISLTLNSTTGKYEGS
                     TSFLTANNEEGGTWKAAWVEVVDLAGNYRVYRITEGYNFYTYVKLTRTSGDISGSFTV
                     TNIVPAAVTRN"
     gene            complement(1189..1488)
                     /locus_tag="PK683_08625"
     CDS             complement(1189..1488)
                     /locus_tag="PK683_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH08554.1"
                     /translation="MSKPKSDLSKKNLETAIGIMDGFSHPVRLTIISALYKEEHTVTE
                     LSELSNVSQSVASQHLAKLRQLGILDSRKELNRVYYYIKDAKAKELYKAVLKLFG"
     gene            complement(1488..2258)
                     /locus_tag="PK683_08630"
     CDS             complement(1488..2258)
                     /locus_tag="PK683_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH08555.1"
                     /translation="MEIKGKTVVITGSGSGLGKAMALGFAKKGANIVVTDINEAAIDS
                     VRKEAESFGVKTIGVQANVSKEEDSIRLMEEAVKQLGSLDVAILNAGILRDGLLVRVD
                     KETGKVKGKMGLDQWQSVIDVNLTGVFLTAREAAVQMINSGKGGVIIPISSVAMHGNA
                     GQSNYSAAKAGVASMTKLWAQELKNYKIRVAGIAPGFIATEMVLKDMKQEALEKWKSL
                     IPIGRLGEPSEISASAEFIVENDLVNGVILEISGGIKI"
BASE COUNT          647 a          531 c          447 g          683 t
ORIGIN      
        1 atgatacgtt cctattcgga aggtactggt ccgactgatg gcgtttggaa actgggaggt
       61 atgtggatca atgataaagc aggaaacggg aaatactaca gcagcaccga tttgaacaaa
      121 aatttcaccc tttacagtgc ctcaactaca caaactgctg atttctatcc tcctgaactg
      181 aagtcgatta caatagacaa gacagatgtc aattttgacc agactttcac tgtctcagca
      241 gatgtttctg atatttccac cgaaaccaca agtggcgatc agaatgccgc atccacttct
      301 gcatcgactg gaacatccac ctctacggcg acgaccacat tgagcagtac ggttgcatct
      361 ggggttaaat cagtaaaggt tgtactctac agtcctctga agcttctgga tgattcactg
      421 ggagtgtcaa aatcaatttc actgactctg aacagtacta caggaaaata cgaaggttcc
      481 acaagtttcc tcaccgcaaa caatgaagaa ggcggaacat ggaaagctgc atgggtagaa
      541 gtagtcgatt tggctggaaa ctacagagta tatagaatta ctgaaggtta caatttttac
      601 acttatgtga aactgaccag aacatcggga gatatctcgg gttctttcac tgtcacaaat
      661 attgttccag cagcagtcac taggaattaa aaaacagctt gtcctgcttc gtgcagttac
      721 tgagtacgaa gcaggacagt gaagacgctt ttatttgcag tcaatcactt gagcagtgaa
      781 attccctccg ttataatttc ctttgccgaa cccccatctc ccggaaatgc cgtcatcgtt
      841 cgcttccctg cataataaaa agtatttcct gcactgatcc agttgtatac gattttgcaa
      901 actggcaccc ttattattgt agcattccct gagacttttg catcaccata ctcatctgat
      961 gcagttccgt catacggaca tcccaggaaa aattactcga cttggcttct cgagtgaatg
     1021 atcccaaaaa agagcataac tctgcggaca aaccggaaca aaaaaaagaa atacagcttc
     1081 tggataaata acttttttca taaaatctcc taagagagca atactcagta ttgatttcat
     1141 tgggtcaaaa aaagttttac ggagttatta gaatgtcttg cagaaaggtc agccgaacag
     1201 tttcagtact gctttataaa gttcttttgc ttttgcatcc ttgatataat agtaaactct
     1261 gttgagttct ttcctggaat ccagaatgcc caattgtctc aattttgcca aatgctgaga
     1321 tgcaaccgac tgactgacgt tggaaagctc agagagctca gtaacagtat gctcttcctt
     1381 gtacaatgca gaaatgattg tgagacggac aggatgggag aacccatcca taattccaat
     1441 tgctgtctcc agattttttt tggataaatc ggatttcggt ttggacatca gattttgatt
     1501 cccccggaaa tctcaagaat cactccgttg accagatcat tttccacaat gaattctgca
     1561 gaggctgata tttcagaggg ttcaccaagt ctgcctatcg ggatcagtga tttccatttt
     1621 tcgagagctt cctgtttcat atccttcaga accatttctg tggcaatgaa acctggagca
     1681 attccggcaa ctctgatttt ataattcttc agttcctgtg cccaaagttt tgtcatggag
     1741 gcaactcctg cctttgcagc agaataattg gactgaccgg cattcccgtg cattgcaacc
     1801 gatgaaatag gaataatgac accgcccttt ccagagttga tcatctgaac agctgcctct
     1861 ctggcagtaa ggaacactcc tgtcaggttt acatcaatga ccgactgcca ttgatcaagt
     1921 cccattttgc ctttgacttt tcccgtttct ttgtcaactc tgacaagaag tccgtctctg
     1981 agtattcctg cattcagaat tgctacgtcc aaagatccca attgttttac tgcttcttcc
     2041 atcaggcgga tggaatcttc ctcttttgac acatttgcct gtacaccgat agtttttaca
     2101 ccgaaagact ctgcttcttt tctcacagaa tcaattgctg cttcatttat atcagtcaca
     2161 actatattgg cacctttttt tgcgaaaccc aatgccattg cttttccaag tccgcttccg
     2221 gaaccagtga tgactacagt tttgcccttg atttccatct aagccttttt tcctttatct
     2281 ctgaatgtta catagtcaga agtgaagg
//
LOCUS       DAOQGJ010000488         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30478_length_2306_cov_3.303865, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000488 DAOQGJ010000000
VERSION     DAOQGJ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30478_length_2306_cov_3.303865"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1205
                     /gene="ychF"
                     /locus_tag="PK683_08635"
     CDS             111..1205
                     /gene="ychF"
                     /locus_tag="PK683_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNH08556.1"
                     /translation="MSLNCGIVGLPNVGKSTIFNALTKAGAQIANYPFCTIEPNKGIV
                     EVPDKRLQRLADIYGPKKIIPALMEFVDIAGLVKGASQGEGLGNKFLSHIREVDAICH
                     VVRAFEDDNITHVHGKIDPIEDIQVVNYELILADLESLEKQHQKVIKTAKTNKESAEI
                     AGVMEKIMNVLREGKPGRLAVLNADEKKISDRFNLITTKPVLYCANILDTEIKNKDNP
                     LLKKIAEFAKSEHAEMIHLSGKVEEELSGLTPEEQMEFLNELGEEESGLNRMIHAAYH
                     LLDLITFLTAGEIEVRAWTTLKGSTAPKAAAVIHSDFEKAFIRAEVIKFEDVDRLGSP
                     AKVKEEGKLRLEGKDYIVQDGDVIYFRVNA"
     gene            complement(1209..1751)
                     /locus_tag="PK683_08640"
     CDS             complement(1209..1751)
                     /locus_tag="PK683_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08557.1"
                     /translation="MKTLNLCGIIFILFSNLFCKGFKATDEDTSFASTDELAALMAAS
                     TANGPCSGSDVKMEYNINYPDQTYDGTREFSVCASAAVDLQRLKFTKAGTYKICLSPG
                     TVTVSCSSSAVRIIGSLNISFTGFDAGTADSFDSSCTSYNVPANKTVSISSKETVDSS
                     SCSGTQTKNVSPNAKAVIKF"
     gene            complement(1752..>2306)
                     /locus_tag="PK683_08645"
     CDS             complement(1752..>2306)
                     /locus_tag="PK683_08645"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNH08558.1"
                     /translation="YWIVVNYREAFGLHASNGILFNHESPRRGENFVTRKITLGVAKY
                     KAGKSKTIHLGNLSARRDWGYAPDYVRMMWMMLQQERPDDYVVATNEMHTVREFTELS
                     FKRAGVEVIWEGKDENEKGINKKNGDVIVAVDPKFYRPTEVEQLLGNPAKAKEKLGWV
                     PNVKFAELVDIMTVADCELFGVKI"
BASE COUNT          662 a          496 c          523 g          625 t
ORIGIN      
        1 gatccggcag actcaggaat tcattcctct caatacaaag gagcataaca ggaagtggat
       61 tcatcctgat aaagagatgc agtcagagct gaaaaaaaaa tagtggtttc atgagtctga
      121 actgtggaat cgtcgggctt cctaatgtag gcaagtccac tatattcaat gctctcacaa
      181 aggcgggagc tcaaattgca aattatcctt tctgtacgat agaaccgaac aagggaattg
      241 tggaagttcc cgacaaaaga ctgcagaggc ttgcagacat ttacggtcct aaaaaaatca
      301 tacctgcact tatggaattt gtggatattg caggtcttgt gaagggtgcg agtcagggag
      361 agggtcttgg aaataaattt ctttcacata tcagggaagt ggacgcgatc tgtcacgtag
      421 tccgtgcctt cgaggacgac aatatcactc atgttcacgg aaagatagat cctattgaag
      481 acatccaggt ggtcaactat gaactgatcc ttgcagatct ggaaagtctt gaaaaacagc
      541 accagaaagt cataaagaca gcaaagacga acaaggaaag tgcagagatt gccggagtca
      601 tggaaaaaat catgaatgtg ctcagagaag gaaagccggg aaggcttgct gtcctgaatg
      661 cagatgaaaa aaagatttcc gaccggttca atctgattac tacaaagccc gtactgtact
      721 gtgcgaatat tctggatact gaaatcaaaa acaaggacaa tcctttgctc aaaaaaatag
      781 cggaatttgc caagtcggaa catgcagaaa tgattcatct ctccggaaaa gtggaagagg
      841 aactttccgg tcttaccccg gaagagcaga tggaatttct gaatgaactt ggtgaagagg
      901 agagcggtct gaacaggatg attcatgcag cctatcatct tctggatctg atcacctttc
      961 ttactgcagg ggaaatagag gtcagggcct ggacaacatt gaaaggaagc actgcaccca
     1021 aggcagcggc agtcattcac tccgattttg aaaaggcatt catacgggcc gaagtcatca
     1081 aattcgaaga cgttgaccgt ctcggcagtc cggccaaagt caaggaagaa ggaaaactcc
     1141 gccttgaggg aaaagactat atcgttcagg acggggatgt aatctatttc agggtgaatg
     1201 cctgatcatt aaaacttaat gactgctttg gcattcggtg aaacattttt tgtctgagtg
     1261 cctgagcagg aagaggaatc tacggtttcc tttgagctga ttgaaacagt tttattggcg
     1321 ggtacattgt aagaagtgca ggaagagtca aaactgtctg cagtccctgc gtcaaatcct
     1381 gtaaaactga tattaagtga acctattatt ctgaccgcac tgctggaaca ggaaacagtg
     1441 actgtacctg gggaaagaca gatcttataa gtgcctgcct ttgtaaattt cagacgctga
     1501 aggtccactg cggcgcttgc acagactgaa aattccctgg ttccgtcata tgtctgatcc
     1561 ggatagttta tattatattc catttttaca tctgacccgg agcagggacc gtttgctgtt
     1621 gaggccgcca tcagtgcggc aagctcatca gtggatgcga agctggtatc ttcgtcagtc
     1681 gctttgaacc ctttgcagaa caggtttgaa aataagatga aaattattcc gcagaggttc
     1741 agtgttttca ttcagatttt tactccgaac agttcacagt ctgcgacagt cataatatcc
     1801 acaagttcag caaattttac attaggcacc catccgagtt tttcttttgc cttggcagga
     1861 tttcccagaa gctgttccac ctcagtcggt ctgtaaaact ttggatcaac tgcaacaatg
     1921 acatcaccgt tctttttatt gattcctttt tcattttcgt cttttccttc ccagattact
     1981 tccaccccgg cccttttgaa ggacagttct gtaaactctc ttactgtatg catttcattc
     2041 gtagccacaa cataatcatc cggtctttcc tgctgaagca tcatccacat cattcgaacg
     2101 tagtccggcg catatcccca gtctctcctt gcacttaagt ttccgagatg gattgtcttt
     2161 gatttgcctg ccttatactt cgccactccc aatgtgattt ttctagtcac aaaattttca
     2221 cctcttctgg gagattcgtg attgaaaagt attccgtttg atgcatggag cccgaaagct
     2281 tctctgtagt tgactacaat ccagta
//
LOCUS       DAOQGJ010000489         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30485_length_2306_cov_2.849400, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000489 DAOQGJ010000000
VERSION     DAOQGJ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30485_length_2306_cov_2.849400"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PK683_08650"
     CDS             complement(<1..359)
                     /locus_tag="PK683_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08559.1"
                     /translation="MKKTASAKKKKPVRKGLWEKSLRSFTMVGPVDNSNIREIKSEEG
                     LSPKLIISLQKAKVKSTEKVYHKTNTIIHKIFSVYSTSRSKVPSLYIVMGSVFGINYT
                     YVNLIGVSPDGKRLKTKL"
     gene            572..1327
                     /locus_tag="PK683_08655"
     CDS             572..1327
                     /locus_tag="PK683_08655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH08560.1"
                     /translation="MKEQILQLTEKNIAEEHICCGFSDRKCSAGYENKKEWLQNEFEN
                     GYTFKKLNVRGKVFIEYCPIEISWLPLEGRKYMVINCFWVSGQFKGQGYGKLLLNECI
                     EDAKKQKMNGIIAVSSDKKRPFMSDPKFLKMQGFNIIDEAPPFFKLWALKFNEKAEMP
                     RIKDTARKGVSPDRKGISVYYSETCPFNDYYINESLKKYAEKKSVPLKIHKIRSREDG
                     HKLPVPWMICSIFYNGELVTLEMKPDKYLDKLI"
     gene            complement(1345..1504)
                     /locus_tag="PK683_08660"
                     /pseudo
     CDS             complement(1345..1504)
                     /locus_tag="PK683_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551333.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(1517..2104)
                     /locus_tag="PK683_08665"
     CDS             complement(1517..2104)
                     /locus_tag="PK683_08665"
                     /inference="COORDINATES: protein motif:HMM:NF020431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1826 domain-containing protein"
                     /protein_id="HNH08561.1"
                     /translation="MKKNTSFFKSMDSGSLSSIHNDGISYVILERELSDSIHRSISFA
                     KELNLQFQSDYLSLKHNIETKMRESYDLLKHLFLDTMYCAELYRTVSGKRNFRIKLNT
                     VSSVTCPRFHCDFTVLRMLCTYTGLSTQFIERKGRNFGFPINETLISKEEIISSGTGN
                     IMIMKGEKYQNNAGNGLIHRSPSANEQRILLVIDE"
BASE COUNT          671 a          424 c          462 g          749 t
ORIGIN      
        1 agttttgtct tgagtctttt cccgtccggg ctgactccga tcagatttac ataggtataa
       61 ttgattccga acactgatcc cattacaata tacaaagagg gtacttttga ccgggaagtt
      121 gaatagacag agaatatttt atgaatgatg gtatttgttt tatgatatac tttttctgtg
      181 cttttgacct ttgctttctg aagagaaatg atcagttttg gagaaagccc ttcctccgac
      241 ttaatttccc gaatattaga attgtcgact ggaccgacca ttgtgaatga tctgaggctt
      301 ttttcccata gacctttcct tactggcttt tttttcttcg ctgaggctgt ctttttcatt
      361 cttcttcgtc ctgagacacg aattgaaatt tttactcaaa gtctctattt ctccagtaca
      421 caggacttta aaatctatgc gacttttttt tactgctcag ttttccaatt atgatccgaa
      481 cgcagatcat tgaatctcat cttcgaattt tacagatcag agattcggaa aactgcttga
      541 cattcgagtt ttttatataa atatatataa tatgaaagag caaatacttc agctgactga
      601 aaagaatatt gcagaagagc atatctgctg cggcttcagt gacaggaaat gcagtgcagg
      661 gtatgaaaat aaaaaagaat ggctgcaaaa tgaatttgag aacggataca ctttcaaaaa
      721 actgaatgtt cgaggcaagg tttttattga atactgccca attgagattt cctggctccc
      781 tttggaaggc agaaaatata tggtcatcaa ctgtttctgg gtttctggac agttcaaagg
      841 gcaggggtac gggaagctgc ttctgaatga atgcattgag gatgcaaaaa aacagaaaat
      901 gaatgggatc atcgcggttt cgagcgataa aaaaagacct ttcatgagcg acccgaaatt
      961 cttaaaaatg caggggttca atatcattga cgaggctcct ccatttttca aactctgggc
     1021 actgaaattt aatgagaaag cagagatgcc aaggatcaag gatactgcca gaaagggagt
     1081 gagtcctgac agaaaaggaa tttctgtgta ttattcagag acctgtcctt ttaatgatta
     1141 ttacattaat gagtcattaa aaaagtatgc ggagaaaaag agtgttcctc tgaaaattca
     1201 taaaatcaga tccagggaag acgggcataa acttccagtg ccttggatga tctgcagtat
     1261 attttacaat ggtgagcttg tgacgctgga gatgaaaccg gacaaatatc ttgataagct
     1321 gatttaatat tctgaggatc aagttccgga gctgatcagt tctgcatcta tattgatttc
     1381 gctcatatcg tttacgatga cagcagcttt cagtcctccg tgattgtcca atatatgatt
     1441 caggagtgta gtttttcctg atcctaaaaa aacctgataa aacagtgact ggaagttttt
     1501 tcatattctc accttctcat tcatcaataa ccaggagaat tcgctgttcg ttcgcagaag
     1561 gtgatctgtg aatcagccca ttccctgcat tattctgata tttttctccc ttcataatca
     1621 ttatatttcc cgttcctgaa cttatgatct cttctttact tatcagagtt tcattgatcg
     1681 gaaagccgaa attcctgcct tttctttcta taaactgtgt agacagacct gtataggtgc
     1741 agagcatcct gagtacggta aaatcacagt gaaatcttgg gcaggtgact gaactgaccg
     1801 tattcagttt tattcggaaa tttctcttcc ctgatactgt tctgtacaat tcagcgcaat
     1861 acattgtatc taggaataaa tgcttcagca aatcatagga ttctctcatc tttgtttcta
     1921 tgttatgctt cagtgacagg taatctgact gaaactgcag attcagttct tttgcaaagc
     1981 tgatgcttct gtgaatcgaa tcgctgagtt ctctttccag aataacatag gaaattccgt
     2041 cgttatggat gctggaaaga cttccgctgt ccattgattt gaaaaatgag gtgtttttct
     2101 tcataatccg taaatgcaac tcagtgtcat taattcttgg atctatttaa atgcaacaat
     2161 gtttcattta cggatgaaaa attttcggat tttttgaagc atagcaggtg tgaagaggca
     2221 cattttggtg cggaggatag aacaagccta tgaactattt ctttatagct caccttacgc
     2281 agatacgata tgctgtctga acgctg
//
LOCUS       DAOQGJ010000490         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30504_length_2305_cov_3.483556, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000490 DAOQGJ010000000
VERSION     DAOQGJ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30504_length_2305_cov_3.483556"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PK683_08670"
     CDS             <1..803
                     /locus_tag="PK683_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08562.1"
                     /translation="YRLEPMNWCIHCHAPLESQKKEILGKENGILHNEGINCAVCHIR
                     NGKIVSSEKRNNSPHDTVIDGTLNSSEFCAGCHEFNFPLVREGRFSFSSEPMQSVYSE
                     WKKSGTKKSCQNCHFENHILKGPSSAGWLKKALGDPELTDSGKGLLHLHLEITAKRAH
                     SFPAGDLFRSLSLEFSNFSDFKTLFFAKKFARFYGAGLTDTGTFWNRRLLSDSTLKPD
                     DNEIRITFDDPGIRDIFFRIRFFYHDPELGGKTGAPENSVILYEKKMR"
     gene            complement(1217..1648)
                     /locus_tag="PK683_08675"
     CDS             complement(1217..1648)
                     /locus_tag="PK683_08675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNH08563.1"
                     /translation="MKKPQKFKFSTSIKVNWGDMDAFGHVNNVIFLRYFETARAEFFS
                     HHKIWENSGRNPSDGMVMSHIELDYRKQIVYPEIITVYVGLESVQSRKFTVACSFYNS
                     EKECAASGFGEFIWFDFVNGKMTSVPEKIFQIFNEENSPHP"
     gene            complement(1709..>2305)
                     /locus_tag="PK683_08680"
     CDS             complement(1709..>2305)
                     /locus_tag="PK683_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08564.1"
                     /translation="VFTQNGYKVYSVIGLDSSTGKSGPCNANATYNGCAAGSTVCAYN
                     RNNGYVQYYNLSTATGGSSASICSSDFSPIMTSIVSQAAGSASPYKLSKTPISSTIVV
                     KVKGVQINNSTSNGWVYDATANAISFAGAATPAAGDAVTVTYEYNTSATAMSSGSNQT
                     LTAFIGNASSEEVGKFAFGALLLAGIALAGRSFFSRKK"
BASE COUNT          649 a          451 c          527 g          678 t
ORIGIN      
        1 ggtacagact tgagccgatg aactggtgca ttcactgtca tgcacctctt gaatctcaga
       61 aaaaggaaat tctgggaaag gagaatggta tactgcataa tgaaggaatc aactgtgcag
      121 tgtgccatat cagaaacggg aaaattgttt cttcagaaaa aagaaataat tcacctcatg
      181 atactgtaat tgacgggacc ctgaattctt ctgaattctg tgcaggatgt catgaattca
      241 attttcctct tgtgcgtgag ggcaggttca gtttctcttc tgaaccgatg cagtcagtgt
      301 attccgaatg gaaaaaatcg ggcacgaaaa agagctgtca gaactgtcat tttgagaatc
      361 atatattgaa agggccgagc agtgcaggat ggctgaaaaa agcgttgggt gatcctgagc
      421 tgaccgattc aggaaaaggg cttctccacc ttcatctgga aattactgcc aaaagagcac
      481 attcatttcc ggcgggagat cttttcagat cactttcact tgagttttcc aatttcagtg
      541 atttcaaaac tctgtttttt gcgaaaaaat ttgcccgatt ttacggagca ggtctgacag
      601 acacgggaac tttctggaac aggaggctgc tctctgattc cacgttgaaa ccggatgata
      661 atgaaataag gattactttt gatgatccgg gcattcggga tatttttttt agaatcagat
      721 ttttttatca tgatccggaa ctgggtggaa aaacgggtgc ccctgaaaac agtgtcattc
      781 tttatgaaaa gaagatgagg tgaggctgta cattttttaa accgttttgt cttcgccgtt
      841 cctataggca cttacttttg actgtccttt taaataagcg tatttcctgc ctgtatttgt
      901 gcagcagttc tgccttggcg aaagtcatgt cgctaagcac aggttttgga cttggagcat
      961 attcgtcatc ccttgcgcgt ctaattccga gacttgtctg agtttttggt gaatttcgga
     1021 agaggaaaag cctgtttcag aaagtgctgt ttttccagga gatcgcagga aaaatcaata
     1081 aaatctgttc tctgcgaact ttgtgccttc cggataagct gtgaccggtg aaactagggg
     1141 gattaaagtg aattaggcag ttccagattt gcgagccttc atgccgacgg atatgcaggt
     1201 gaaatgataa aatatctcag gggtgaggag agttttcttc attgaatatc tgaaagattt
     1261 tttccggtac tgaagtcatc tttccgttta caaaatcaaa ccagataaat tctccgaagc
     1321 ctgaggcagc gcattccttt tcggaattgt aaaaagagca ggccactgta aactttctcg
     1381 actgtacaga ttcaagtcct acatacactg taatgatttc gggatataca atctgttttc
     1441 tgtagtcaag ctcaatatgg gacatcacca ttccgtcaga aggatttctc ccggagtttt
     1501 cccagatttt gtgatgagag aagaattccg ctcttgctgt ttcaaaatat ctcaggaaga
     1561 tgacattgtt cacatgaccg aaagcgtcca tatctcccca gttcacttta atggaagtgc
     1621 tgaatttgaa tttttgaggt tttttcaatt tcttaaaggg aaataaaaaa gggaatgcct
     1681 ttcgacattc cctcaggaga gccccagatt atttttttct ggagaagaaa gatcttcctg
     1741 caagtgcgat acctgcgaga agaagtgcac cgaatgcaaa cttgccgact tcttcactgg
     1801 aagcatttcc gatgaatgca gtcagagtct ggtttgaacc tgagctcatt gcagttgcag
     1861 aagtgttgta ttcataagtc acagtgactg catcgcctgc tgcaggagta gctgcccctg
     1921 caaaggagat tgcatttgca gttgcatcat atacccatcc atttgaagtg ctgttgttga
     1981 tctgcacacc tttcactttc actacgatag tggaagaaat tggagtcttg gagagtttgt
     2041 aaggacttgc actgcctgca gcctgagata cgatgcttgt cataatcggg ctaaagtcag
     2101 aggaacagat agaagcagaa gaacctccag tagctgtaga caggttgtaa tactgaacat
     2161 aaccgttgtt tctgttgtaa gcacagactg tagaacctgc agcacatccg ttatatgtcg
     2221 cattagcatt acatggaccg gatttaccag tagaagaatc aagtcctatg actgaataaa
     2281 ccttgtatcc gttctgagta aaaac
//
LOCUS       DAOQGJ010000491         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30508_length_2305_cov_2.746667, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000491 DAOQGJ010000000
VERSION     DAOQGJ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30508_length_2305_cov_2.746667"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PK683_08685"
     CDS             <1..251
                     /locus_tag="PK683_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08565.1"
                     /translation="CISALYRLILKWCIINTIPIKNLEEVYIKHYNIAYRGVFPLFSK
                     RTKRGTRAVKMNVKAEIKKELSEHGIDHSTIEFEPVSE"
     gene            576..1208
                     /locus_tag="PK683_08690"
     CDS             576..1208
                     /locus_tag="PK683_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH08566.1"
                     /translation="MNLKVKIALIEDQPSVCETLETILSKNPNIEKVISFHSAEECEA
                     SDISEIDIFVIDVRLPGMDGITFLSRINDRRPESKKLIMTMIESDDIIFQALKLGCIG
                     YVLKTDIDKISEIIDITAAGGAYMSPSAAIRVMNFFYRIKNNDEYEKLTEKETQILKE
                     LSAGYTPAEIAEALKVTVSTVRFHIRGIYQKLQVNNRIQMLKRAEGLGII"
     gene            complement(1464..1922)
                     /locus_tag="PK683_08695"
     CDS             complement(1464..1922)
                     /locus_tag="PK683_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08567.1"
                     /translation="MKKFLVISMILACTAAFAKDTKKDDKKKDDKKEMGESKMAGKTV
                     SLELKVKFPPKNNEGKSWDTFGGANQKITIKDGNVLGGSCDKFGKSPDRGLTEMWKCK
                     NVKLDNEGQFPLEIQEKDAVSNDYVGNGRLTATKKGEQKIGSASVWVEIK"
BASE COUNT          712 a          405 c          429 g          759 t
ORIGIN      
        1 gctgtatctc tgcgttatat agactcatcc taaaatggtg tattataaac accattccaa
       61 tcaaaaacct tgaagaagtg tatataaaac actataatat agcctatcga ggggtgtttc
      121 ccttattttc caaacgtacc aaacgaggta caagggctgt aaaaatgaac gtcaaagcag
      181 aaataaaaaa agagctttct gagcacggta ttgatcacag taccatagaa tttgagcctg
      241 tgtcagagtg atatttcgtg taccgtccga atattccagg aaacccctaa aaacggttat
      301 tttagcaaag acaattgaaa atttcctggg aaattttatc ctttcacttt agaggttaat
      361 agaggttccc tctaattatc gtaaaactga attttgacag aaatctgttt cactgttttt
      421 aagatcctaa aaaatgcaca cctaagtaat gacaatcgac cttaaaccct atcaattttg
      481 acaggttttc atgattgccg caaaaatttt tcaacatgat ttcttttttt aattgattat
      541 ataaaatcat atagatgatt attcgatagg tgtgaatgaa tttgaaagta aaaatagcct
      601 taattgaaga tcagccgtct gtctgcgaaa ccttggagac tatactctca aaaaacccca
      661 atatagaaaa ggttatcagc tttcattccg ctgaagaatg cgaagcttct gatatttcgg
      721 aaattgatat ttttgtcatt gatgtaagac ttcctggaat ggatggaatt acttttctgt
      781 caagaatcaa tgatcggcga ccggaaagta aaaaactgat tatgacaatg atagagtcgg
      841 atgatatcat ctttcaggca ctcaagcttg gatgcatcgg ctatgtcctg aaaactgata
      901 ttgataaaat ttcagagatc attgatataa ctgcggcggg cggcgcctat atgagcccaa
      961 gtgcagcgat tcgggtaatg aatttttttt acagaatcaa aaacaatgat gaatatgaaa
     1021 aactgactga aaaggaaaca cagattttga aggagctttc ggcaggttat actcctgcag
     1081 agattgcaga agcactgaaa gtgacagtat ccactgtaag gtttcatatt cgcgggattt
     1141 atcagaagct tcaggtcaac aacagaattc aaatgctgaa aagagctgaa ggtctcggca
     1201 ttatataagt attcagatct gccagaactc tttccataag atcaatgcaa gagttctctg
     1261 atatgcacaa tatatcgata tgtgtgaatt tttatatgca tttatatggg aaaagtaaaa
     1321 aaacttatca aaagacggca aaaatgtgag gggattcggg cgagaaacac tcaggcgtga
     1381 atggaatttt agtttcagga atttaaagag aggaataaat aaaagcccgc tttgcagcgg
     1441 gcacacagat tctgtgaatt aaattacttg atttctaccc atacggatgc acttccgatt
     1501 ttctgctcac cttttttagt tgcagtcagt cttccgtttc ctacatagtc attggaaaca
     1561 gcatcttttt cctggatttc aagagggaat tgaccttcat tgtccagttt tacgtttttg
     1621 catttccaca tttcagtgag acctctgtct ggagattttc caaatttatc acatgaaccg
     1681 ccgagaacgt ttccgtcttt gattgtgatt ttttggttag caccgccgaa agtatcccag
     1741 cttttgcctt cattgttttt tggaggaaat ttaactttca gttcaaggct gacagttttt
     1801 ccagccattt tgctttctcc catttctttt ttgtcgtctt tctttttgtc gtcttttttt
     1861 gtgtcttttg caaaagcagc tgtgcaagct aaaatcatgg atattacaag aaactttttc
     1921 attcaatact ctcctagttt gtatcgaagg ccctattgcc gaaccttcat ttcttagact
     1981 gttctttaaa cctctctgaa acggatagtg gaaaatttta caaaaaaaga aaattttttt
     2041 aactcttttt tttagatgaa ttctttaagg ctggtttttt tggtatgatt tttgccggac
     2101 attttttgct gtcagcaata aaagacagat tcagtcttca gagcagaata ttttattatg
     2161 tatataatat aattgtctta tttgagacca gattcagtct cctccttgaa caggtgccgg
     2221 tatttatctt aggctgaaca gaaatataat ctatgtattt atgattaagc tgatcatata
     2281 atgccttcgg ctgcaggttc gagga
//
LOCUS       DAOQGJ010000492         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30697_length_2297_cov_3.499554, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000492 DAOQGJ010000000
VERSION     DAOQGJ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30697_length_2297_cov_3.499554"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..521
                     /locus_tag="PK683_08700"
     CDS             72..521
                     /locus_tag="PK683_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08568.1"
                     /translation="MEETGSYLQFFLGIAALALITAAVRYILKTGFRRADEFEVFNRV
                     FRTEREPAGRVLKNQYGQLGYMKYKGYLKIIINENGIYFNTESGFAKFGNPLFIPWKS
                     LRFKSSSSFFFEKYIQLEADCGDKSPKVEIELSEKVFTPLEIQRLKK"
     gene            complement(681..>2297)
                     /locus_tag="PK683_08705"
     CDS             complement(681..>2297)
                     /locus_tag="PK683_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08569.1"
                     /translation="RIQPMASYVSRLKKEPCLYLYLPDHIGKGEELELEAGYFTDRLS
                     KLICRNGTVQEKEIQGKGIYTFHYKYGQETEIQLFSGASEIRKKVEIPEQKFKIVSFS
                     YLPPETKVSDNGTVFSLYKSPSQLSDVILQEFLLKYPWGCGEQTAAKLVGFSNLLLKV
                     IPVSADHFLVQKIKSGIKALEAYRNESGFYSIFPGAPADEKTTTYIYNNLKLLFPHRA
                     VLEPLLPELWKSIDQMKSKISKAEEGEGRSEIPGPLNTHNEKSILKALKTSVIFEKGK
                     LKNKSFSAYQTLASKVFAASNLLLEKSKNEIEVLAGTEKKVKEIHSDGIRGIAESIGL
                     LDKKKQEITVSKTVRLSDPMDLLLSAGEIVFLEGSFPSTIEQMSFLVFLDTLKTKMNE
                     YHFMKNGKSYSPENDEELKDITVKSEYTFAKKTVTLGPTPAPHSSVPEISFSSLSFEK
                     GDILRLNIKRKHPTEIYHLICPGMFEPVYCSEFHSPSYSRILISPMFGGTEIPLRTVR
                     KGKGKFFLLAEDMYETGRVYMKELQVIEVI"
BASE COUNT          657 a          469 c          448 g          723 t
ORIGIN      
        1 gccggtcaag cggattttga gtcagtaaat ttttctttac aaataacagc tttccttttg
       61 cttcttctgt tatggaagaa acaggttcgt atctgcagtt tttcttaggc attgctgccc
      121 ttgcattaat tacagcagca gtccgttata ttctgaagac agggttcaga agggctgatg
      181 aatttgaggt attcaacaga gtatttcgga cagagagaga acctgcagga agagttctga
      241 aaaatcagta tgggcagctc ggttatatga aatacaaagg atatctgaaa atcattatta
      301 atgaaaatgg gatatatttt aatacagaat ccggatttgc aaaatttgga aaccctctct
      361 ttattccctg gaaaagtctc agattcaagt cttcctcaag tttttttttc gaaaaataca
      421 ttcagctgga agccgactgc ggagacaagt ctcccaaggt ggaaattgaa ctttctgaaa
      481 aagtattcac tcctttggaa atccagagac tgaaaaaatg aacggctgaa acttattttt
      541 cctccggcat ttttctcccg cgctcccctg cgcttcgaat cggtatatga caggatttcg
      601 attccataca gtatcactgt cataattaat aagaatgaaa tcataaggct ccttataagc
      661 tgaaagaatt ctcatcgctc tcatatcact tcaatgacct gaagctcttt catatagact
      721 ctgccagttt catacatatc ctctgccaga agaaaaaatt tccccttccc ttttcggaca
      781 gtgcgaagag gaatttctgt tccgccgaac attggactga tcagaattct cgaatatgac
      841 gggctatgga attctgaaca gtagaccggc tcaaacattc cgggacagat cagatgataa
      901 atttcagtag gatgttttct ttttatattc agtctgagga tgtctccttt ttcaaatgag
      961 agagaggaaa aactgatttc agggactgaa gagtgagggg caggtgtcgg tcccaaagtg
     1021 actgttttct tcgcaaaagt atattcagat tttacagtaa tatccttaag ctcctcatcg
     1081 ttttccggag aatagctctt tccgtttttc ataaaatggt attcattcat ttttgtcttc
     1141 agtgtatcca ggaaaacaag aaaactcatc tgctcaattg tagaaggaaa gcttccctcc
     1201 aaaaaaacaa tctctccggc agaaagcagc agatccattg gatcagaaag acggacagtt
     1261 ttacttacag taatttcctg cttctttttg tccagaagac ctattgattc tgcaatacct
     1321 cgtatcccgt ctgaatggat ctctttgact ttcttttctg tgccggccag aacctcgatt
     1381 tcattcttag atttttccag aagcagattg gatgcagcaa agaccttgga agcaagggtc
     1441 tgataagcag aaaatgattt attctttagt tttccttttt caaaaattac ggatgtcttc
     1501 aatgctttca gaatgctctt ctcattatga gtattcaatg gtccgggtat ttcagacctc
     1561 ccttctcctt cctcggcctt cgaaatcttt gacttcatct gatcaatact cttccagagt
     1621 tccgggagca gtggttccag tactgctctg tgagggaaga gaagctttaa attattatat
     1681 atatacgttg tagttttttc atctgctggt gctccaggaa aaatactgta aaatcctgat
     1741 tcatttctgt aagcctccag agcttttatg cctgatttta ttttctgaac aagaaaatgg
     1801 tctgcggaaa cgggaattac tttcaaaagc agattcgaga aacccaccag ttttgcagca
     1861 gtctgttctc cgcatcccca gggatatttg agcagaaatt cctgaaggat cacatcagaa
     1921 agctgacttg gagatttata aagactgaat acagttccat tatcagagac ttttgtttcc
     1981 ggagggagat aagagaagga aacgattttg aatttctgtt caggaatctc aactttcttt
     2041 ctgatttcag atgcacctga aaaaagctga atctctgtct cctgaccata tttataatga
     2101 aaagtatata tccccttccc ctgaatttcc ttttcctgaa ctgtgccgtt tctgcagatc
     2161 agcttagaaa gccgatcggt gaaatagcct gcttcgagtt caagttcttc tccttttccg
     2221 atatgatccg gaagatacag ataaaggcag ggttcttttt tcaaccggga cacataagaa
     2281 gccataggct gaattcg
//
LOCUS       DAOQGJ010000493         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30787_length_2293_cov_3.361483, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000493 DAOQGJ010000000
VERSION     DAOQGJ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30787_length_2293_cov_3.361483"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PK683_08710"
     CDS             <1..1024
                     /locus_tag="PK683_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNH08570.1"
                     /translation="IIAPVEDTGQLSCYDNSASQVCGTVSATFPKQDAMMLNVPVAKG
                     LQTSTVNTIYPNDYITKNTLTGIVWKTCAEGKSGSTCATGTASSLNYAGAVAACSALN
                     TVNGGAGYAGLKNWRLPTGQEFVQVQNYNTASVYWDTTYFPGAANSDLWASNILLPSG
                     TFGTVIDSSFNGVPTVQAASSSYAVRCVSGSPFPAPSLADNGDGTILDKRTGLIWQKC
                     AYGQANDATCSTVPTPITWANALLTCSSLSLAGKSWRLPNIAELSSIIDTVTKTAPPY
                     VNSVFAGFPEGGQIRNFHSSTTAVYNTMYNFKVEYHTPKGVTADTKNNTVVTSNDYLS
                     RCVSGP"
     gene            complement(1147..1710)
                     /locus_tag="PK683_08715"
     CDS             complement(1147..1710)
                     /locus_tag="PK683_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08571.1"
                     /translation="MKITGLKIFFALGIILASADCTQSKTDNSKSSTSALAGLVGGVS
                     SEQAKDFVLNGSWNSFIGSSTTAIGTININAKSNSGVEVDDGTGFSACYLIAEFSNTS
                     VPGYYVSQNPSSNGACFSPDTNKGKYNYNVFVKDSSKANTYWVCTIGFGKSSVTEAKA
                     SSLTNLSTVNLTTTGCNGSSWTRMEKK"
     regulatory      complement(1847..1988)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          680 a          524 c          458 g          631 t
ORIGIN      
        1 aatcattgcg cctgtggaag acacaggaca gctttcctgt tatgacaatt cagcatctca
       61 ggtatgcggt acagtttctg caacattccc aaagcaagat gcaatgatgc tgaatgtgcc
      121 tgtcgcaaaa ggacttcaga catccactgt aaatacaata tatcccaacg actatattac
      181 taaaaacact ctgacaggga ttgtctggaa aacctgtgca gaaggcaaaa gcggttctac
      241 ttgtgcaaca ggaacagctt cgtccctaaa ttatgcaggc gcagtggctg catgttctgc
      301 tctgaatacc gtgaacggag gagccggtta cgcaggactg aaaaactggc gtctgcccac
      361 tggacaggaa ttcgtacagg tgcagaacta caatacagca agtgtttatt gggatacaac
      421 atatttcccc ggagctgcaa attcagattt gtgggcttcc aatattctgc tcccttcagg
      481 aacattcgga acagtcattg attcatcatt caacggagtc cctactgtcc aggcagcaag
      541 ttcatcatat gctgtaagat gcgtatccgg cagccccttc cctgcaccgt ctctggctga
      601 caacggggat ggaaccattc tggacaaaag aacaggactc atctggcaga aatgtgctta
      661 cggtcaggca aatgatgcca cctgctccac tgtaccgaca ccaatcacct gggcaaatgc
      721 tcttctgacc tgcagcagtc tttcactcgc aggaaagtcc tggagacttc cgaatatagc
      781 agagctttct tccatcatag acactgttac taaaaccgct ccaccctatg taaattcagt
      841 atttgcagga tttcctgaag gcggacagat acgcaacttt cattcgtcca ccacagctgt
      901 ttataatacg atgtataatt ttaaagtgga ataccacacc ccaaagggtg taacggcaga
      961 cacaaagaac aacacggtag taaccagcaa tgattattta tcccgctgtg taagcggacc
     1021 ctgatcagac ctttgtctgc acgaaataca tgaagcacag catactgaat aatttaatac
     1081 aattatccag tatgctgatt attgtctggt gcaaattcga atctgcaccc ctgattcatt
     1141 caaatgttac tttttttcca tccttgtcca ggatgatccg ttgcatcctg tagttgtcag
     1201 gttgacagta ctcaggtttg tgagcgagga agccttagct tcagtcacac tgctttttcc
     1261 aaacccaatc gtacagaccc agtatgtatt ggccttggaa ctgtctttca caaatacatt
     1321 ataattatac ttccccttat tcgtatccgg tgaaaaacat gctccgtttg aggacgggtt
     1381 ctgagaaaca tagtaaccag gaacagatgt gttggaaaac tctgcaatca gataacatgc
     1441 actgaatcct gttccgtcat ccacttcgac tcctgaattg gatttggcat tgatattgat
     1501 cgttccaatt gcagtcgttg aacttccaat aaaagagttc cagctcccgt tcagtacaaa
     1561 atcctttgcc tgttctgaag aaactccgcc cacaagtcct gccagtgcag atgttgaact
     1621 tttcgaattg tctgtcttag actgagtaca gtctgccgat gccaggatga ttcccagcgc
     1681 gaaaaaaatt tttaaaccag tgattttcaa tggtttctcc tgtatttttt ttcctttgga
     1741 gccctcagag aagccggaca tctcagcaca gaaatcatca tgatattgca aataaaatct
     1801 ctgtaaaaaa aatgaaacgc caaacccagt ataaagtttt cctttccacg agaaaacgtt
     1861 atgagaggag tatctgacta cagaagcata acttcttata cagttgcggg acagtgcagg
     1921 attcacacct gcttcctccg ttcggataat tgcatttttt ttgaaagccc tgatccgtca
     1981 ataggaaaaa acgctgagcc tgctgagatg ctttggggtt tcgaatttat tgagagtacc
     2041 ttgattttgt tattgttcca aattgccgac cagtccggaa tctctaagct gtgcgcaggc
     2101 aaataaattg acaaattcat attttttcat taaaaagaaa aaatacgtta cagaaatatg
     2161 gtacagtttc agagtattga aaaacaatat caggaagatt aaatgaaaac taaactctca
     2221 aatagtatac aatgtattgc agtatttgcc ttattcagtc tgacattctg cagtaaaaaa
     2281 gaagacagca gcc
//
LOCUS       DAOQGJ010000494         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30882_length_2289_cov_2.787825, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000494 DAOQGJ010000000
VERSION     DAOQGJ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30882_length_2289_cov_2.787825"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PK683_08720"
     CDS             <1..500
                     /locus_tag="PK683_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNH08572.1"
                     /translation="KGPYAMEHKNFIAKGRFWEREKSKYESYRREDGMEVYGGDNHYA
                     GMELLRFIQYGLRLQTDQKTDRFTEPAKIVHPYISTYSLPTLINSISAYLELGEIRSD
                     RDIYFLKHKKKKMSVCIAVGIYSLLNGLEIRSKQDFPYIPKGKKLDLGKYITEKGTYY
                     FMEAR"
     gene            505..1263
                     /locus_tag="PK683_08725"
     CDS             505..1263
                     /locus_tag="PK683_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08573.1"
                     /translation="MDTSFIKDPRFAKIITAYRKSLVERYSPKNISRFPEFNGMKRET
                     IDLLIQYFLELLYPEYEVRKELDNAFQALAAFTHSPAKLFGVMGNIAGAVFKFGKLLI
                     PAMQAGINSLRSYVAANNFEKDLFMHAIPLIDKGTDISEEKVFMSLVANIPQVEADKF
                     RYQIVKLFEILSQKHLPEKALEVMQSVKHKMETKRNLYTDQEIEGIQMGCNIIEKGKF
                     IFTELNDSEIALVLKGIDRIEEDFYHRCVASVKI"
     gene            1323..2063
                     /locus_tag="PK683_08730"
     CDS             1323..2063
                     /locus_tag="PK683_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH08574.1"
                     /translation="MDNFDSALSKAKKDKKPVLIDLYTDWCGYCQVLKKKIFPAREVE
                     SELKNFITVRINGDEVPEIPYMFQVKGYPTILFLDKNGAYLDRLTGLPDVKIMTAKLK
                     KVYEKKDLEQDLLKEAKKNPEGVAVNFRLGIYYYKISSREKAISYFMKSVNSPRKENP
                     EKKMESLFNIGIIQMESKKFSEAVSIWTKYIEDYPRGDISSAYYYRGVAHMKGGQSKE
                     AKSDFESSLKAGADSETREKIEGYMKQL"
     gene            2066..>2289
                     /locus_tag="PK683_08735"
     CDS             2066..>2289
                     /locus_tag="PK683_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08575.1"
                     /translation="MELNHPELTLAALKILRSGENFQWYIIPILALVVYVYSNEAAAK
                     NWRGIASGLCLYSIHWFFEILNGLIQHFSG"
BASE COUNT          786 a          449 c          497 g          557 t
ORIGIN      
        1 aaaaaggccc ctacgcaatg gaacataaaa acttcatcgc caaagggcgg ttctgggaga
       61 gagaaaaaag caagtatgaa tcttacagaa gagaagacgg aatggaagtg tacggaggcg
      121 acaatcatta tgcggggatg gaacttctcc gtttcatcca gtacggacta cgtcttcaga
      181 cagaccaaaa aacagacaga ttcacggaac ctgcaaaaat tgtgcatcct tatatttcca
      241 catacagcct gcctacactc atcaattcca tttcggcata tctggagctg ggagaaatca
      301 gatcagacag agacatttat tttttaaaac ataaaaaaaa gaagatgtca gtctgtatcg
      361 cagtcgggat atattctctt ttgaacggac tggaaatcag gtccaagcag gattttccgt
      421 acatcccgaa aggaaagaaa ctggacttgg gaaagtatat tacggaaaag ggaacatatt
      481 attttatgga agcaaggtga aagaatggat acctcattta tcaaagatcc cagatttgca
      541 aaaataatca cggcatacag gaaatctctt gtggaaaggt attcaccgaa aaatatttcg
      601 agattccctg aattcaacgg aatgaaaaga gaaaccattg atctgctcat ccagtatttt
      661 ctggaactgc tttaccctga gtatgaagtg agaaaagaac tggataatgc gtttcaggct
      721 cttgccgcat ttactcacag tcctgcaaaa cttttcggag taatgggaaa cattgccggt
      781 gcagtattca aattcggcaa gcttctgatt ccagcaatgc aggcaggcat caactctctc
      841 agaagttatg tagccgcgaa caattttgaa aaagatctgt tcatgcatgc aatccctctg
      901 attgacaagg gaacagacat cagtgaagaa aaagtattta tgtcccttgt tgcaaatatt
      961 ccccaggtgg aagcagacaa attcagatat cagatagtca aactctttga gatactttcc
     1021 cagaaacatc tgcctgaaaa agctctggaa gtaatgcagt cagtcaaaca taagatggaa
     1081 acaaaaagaa acttatatac cgatcaggaa atcgaaggga tacagatggg atgcaatatc
     1141 attgaaaagg ggaagtttat atttactgaa ctcaatgaca gtgaaatagc acttgtactg
     1201 aaagggatag acagaattga ggaagacttc tatcatagat gcgtagcctc ggtgaaaata
     1261 tgaaatattt gctcctgttt atgttccttc cattcttctc tgtttcagcg gaaaggaaat
     1321 ggatggataa ttttgattca gctctatcca aagcaaaaaa ggataagaaa ccggttctga
     1381 tcgatctgta tacagactgg tgcggatact gccaggttct caagaaaaaa atatttcctg
     1441 caagagaagt cgaatctgaa ctgaaaaatt tcatcactgt cagaatcaac ggagatgagg
     1501 tacctgagat cccttacatg ttccaggtaa agggataccc tacaattctg tttctcgaca
     1561 agaacggcgc ttatctcgac cgacttaccg gactgcccga cgtcaaaatc atgaccgcaa
     1621 aactgaaaaa agtgtatgaa aagaaggacc tggaacagga tcttctgaaa gaggccaaaa
     1681 aaaatccgga gggagttgca gtcaatttca gactcggaat ctattattat aaaatcagca
     1741 gcagggaaaa ggcgatcagt tattttatga aaagcgtgaa ctctcccagg aaagaaaatc
     1801 cggaaaagaa aatggagtct cttttcaaca tagggatcat ccagatggaa tccaaaaagt
     1861 tcagcgaagc tgtgagtatc tggacgaaat acattgaaga ctatcccaga ggagacatct
     1921 cttccgcata ctactacaga ggagttgcgc atatgaaagg aggacagtcc aaggaagcga
     1981 aatcggattt tgagtcctct ctgaaagcag gagcagactc tgaaaccaga gaaaaaatcg
     2041 aaggatatat gaaacagctg taactatgga actgaatcac cccgaactga ctcttgccgc
     2101 actcaaaatc ttaaggagcg gcgaaaattt tcaatggtac atcatcccca ttcttgcgct
     2161 cgttgtatat gtgtattcca atgaagctgc cgcaaagaac tggaggggga tcgcatcagg
     2221 actctgcctt tattccatac actggttctt tgaaatactg aatggactca tccagcattt
     2281 ttcgggcca
//
LOCUS       DAOQGJ010000495         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30901_length_2288_cov_2.453650, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000495 DAOQGJ010000000
VERSION     DAOQGJ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30901_length_2288_cov_2.453650"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PK683_08740"
     CDS             <1..1160
                     /locus_tag="PK683_08740"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNH08576.1"
                     /translation="TGTAAALLKTVGLQPNLPVLNIILPLGISFYTFQTMSYTVDVYR
                     GQLEPADDFWDFALFVTFFPQLVAGPIERATNLLPQVLKERTIVFQELQNGAFLILFG
                     FFKKLFIADNLAMIVDPIFDPIEKGTLNFGEAGPPNSPTVLFGTVAFTIQIYCDFAGY
                     SDIARGISKFMGFELMRNFNTPLLAQNINDFWRRWHISFMSWLRDYVYFSIGSKNDPE
                     WRKNANNLFTYFLSGLWHGASWTFAIWGLVNGICSVLYKLTLPLWPKSRGDHYKIISI
                     SKKIIKALFTLVLISAPTIYFRSQSFDLALLHSKSLFTNFGLLDAKLFEKVFRNIALL
                     LLIEIYQNSTEDEYAVFRLPVAVRSVLYVVMFYAVTVLGNFNKNAFIYFVF"
     gene            1135..2199
                     /locus_tag="PK683_08745"
     CDS             1135..2199
                     /locus_tag="PK683_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNH08577.1"
                     /translation="MHSFISFFKKSPAFVLAVLFIVLFETALHFVPNLHYLDGRGAFF
                     TYYKRFLAEDSTKSYDILIYGDSRSLSLQGQKKDDKNKYSIYNFSLPAAGPRYFPYFL
                     KKYLKNHSEKPKLVVWAADPALMMSKKTVSFSDNPALWNEYKHRLLNLFSISEAWEQY
                     SGYEFFFIMKEYLPHSILTVRHRQGFESLVGIRLESLLSGKFHLVEENKKLIKFVEEE
                     NGKINLGDFFPAPEGASEREIVNHLPVYNTSEINTEPLRNFLDQCRKENLKAIVLNIP
                     RAEGFHQTLFYRTAEAEIKRLLESYPEFRYIQFEDMDYPLNLFSESIHYNFKGGKRVN
                     AEYTEKIVPQFYSYIENNEK"
BASE COUNT          633 a          468 c          458 g          729 t
ORIGIN      
        1 ttacaggaac cgctgcggca cttttaaaga cagtaggact tcagcccaat ctgcctgtcc
       61 tgaatatcat cctgcctttg gggatttcct tttatacatt tcagacaatg tcttataccg
      121 tggacgttta caggggacag ctggaaccag ctgacgattt ctgggatttt gcactctttg
      181 tcactttttt tccgcagctt gtcgcaggcc cgattgaaag agcaaccaat ctgcttcctc
      241 aggttctgaa agaaaggaca attgtttttc aggaactgca gaacggtgca ttcctgattc
      301 tcttcggttt cttcaagaaa ctcttcattg ccgacaacct ggcaatgatt gttgatccta
      361 tcttcgatcc gatagagaag gggacattga atttcggaga agcaggtcct ccgaattctc
      421 ctacagttct gttcggcact gttgcattta caattcagat ttactgtgac tttgcgggat
      481 attccgatat tgcacgagga atttccaaat ttatgggctt tgaactgatg agaaatttca
      541 atacacctct tctggcacag aatataaacg atttctggag aagatggcat atctctttta
      601 tgtcctggct tcgggactac gtttattttt cgatcggaag caagaacgat ccggagtgga
      661 gaaaaaatgc aaataatctg ttcacgtatt ttctgtcagg gctgtggcac ggagcaagct
      721 ggacatttgc aatctggggt ttggtgaacg gaatttgcag cgttttatac aaactcactc
      781 tccctctttg gcccaaatcc cggggagatc attataaaat catttctatc tcaaaaaaaa
      841 tcattaaagc tctctttaca ttggtgctta tttctgcacc tacaatttat ttccgttcgc
      901 agagttttga tctggcactg cttcattcca agagtctgtt tacgaatttc ggcctgcttg
      961 atgcaaagct gtttgaaaag gtattcagaa acattgctct tcttctgctg attgaaattt
     1021 atcagaacag tacagaagat gagtatgctg ttttccggct gcctgtcgcc gtccgttctg
     1081 ttctgtatgt cgtgatgttt tatgccgtaa ctgtcctggg gaatttcaat aaaaatgcat
     1141 tcatttattt cgttttttaa aaaaagccct gcctttgtat tggcagttct ctttattgtg
     1201 ctgtttgaaa cagcacttca ttttgtaccg aatctgcatt acctggacgg gagaggtgcc
     1261 ttcttcactt attacaagcg ttttcttgca gaagacagca caaaatccta tgatatcctg
     1321 atatacggag acagccggtc actttccctc cagggtcaga aaaaagatga taaaaataaa
     1381 tattctatat acaacttcag tcttcctgca gccggaccca gatattttcc gtatttcctg
     1441 aagaaatatc tgaaaaatca ttctgaaaag ccgaaacttg tggtctgggc agccgacccg
     1501 gctctgatga tgtccaaaaa aactgtttcc ttttcggaca atcctgcact ctggaatgaa
     1561 tacaagcaca gacttttgaa tcttttttcc atatctgaag cctgggagca gtacagcgga
     1621 tacgaatttt tctttattat gaaagaatat cttcctcatt ccattctgac agtgagacac
     1681 agacagggtt ttgaatctct ggtcggaatc aggctggaaa gtctgctgtc cggaaaattt
     1741 catcttgtgg aagaaaacaa aaaactgatc aagtttgtgg aagaggaaaa tggcaaaata
     1801 aatctggggg atttctttcc tgcgccggag ggtgcgtctg aaagagaaat agtcaatcat
     1861 cttcctgttt acaatacttc ggaaatcaat acggaacctc ttcgaaattt tcttgaccag
     1921 tgcagaaagg aaaatctgaa agcgattgtt ctgaatatcc cccgtgcaga aggatttcat
     1981 cagactctgt tttatagaac ggcagaagct gaaataaaga ggctccttga gtcttatcct
     2041 gaattcagat acattcagtt tgaagatatg gattatcctt tgaatctgtt ttctgaatcc
     2101 attcactata attttaaagg cggaaaaaga gtaaatgcgg aatacacaga aaagattgtc
     2161 ccgcagtttt actcttatat agaaaataat gaaaaataaa tttcttttat acccagtttt
     2221 tcttctgctt tttctctttc ttttcgacaa gatctttctt atcgaatttt tcaagcctta
     2281 tatcaagc
//
LOCUS       DAOQGJ010000496         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30921_length_2287_cov_3.347222, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000496 DAOQGJ010000000
VERSION     DAOQGJ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30921_length_2287_cov_3.347222"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PK683_08750"
     CDS             complement(<1..700)
                     /locus_tag="PK683_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08578.1"
                     /translation="MRKLIDFSLILKAETVRDFIFMKRYPAETISFLFFMYLILLAIL
                     FGFHQLAGSASGVLNKDKMILGYSLMQFVMATQMGWSGQIQNESQTGTLEQLSISGHT
                     LGEVLLARGLSQMPRQMLSFYVLLGAYTFSIENISLSLSPHIPQTILLLLIMGIGIFG
                     LSYIFAGITLIFKRVGFFFQIINFAFLGLFWQDRTKLADGGAIAFIYDHFPLTLGMAN
                     LQKLLTGTSGPGFAD"
     gene            complement(697..1332)
                     /locus_tag="PK683_08755"
     CDS             complement(697..1332)
                     /locus_tag="PK683_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNH08579.1"
                     /translation="MNFLDRSVRDFVTEDILSIKSEKEKAKALYYKVRDEIRYDPYHI
                     LLKPEFLRASEIVKRRYGFCVEKALLLAAVSRLAGIPSKIGFANVRNHISSPRLSSLM
                     KTDIFVYHGYTEMFIEGKWVKCTPAFNKSLCDRAGIFTLEFDGENNSLFHPLDLQGKK
                     HMEYLQDFGTRDDLPYQEMMDKYAEAYPHLDVKETGTFGPVKITAKFEDEV"
     gene            complement(1361..>2287)
                     /locus_tag="PK683_08760"
     CDS             complement(1361..>2287)
                     /locus_tag="PK683_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNH08580.1"
                     /translation="EGENPEIWKILPDFAKQGIEKQIGEVIPKQIVEVYKSFGDDLDS
                     VLEFDEVVKDSLCGKNVGVLIELFKRCGGPEFRFLVVSGIYFGFLIGLIQLAFLSILG
                     QWWTMPIMGVIVGYVTNWLALQMIFRPLEVTNFGFFKYQGLFLRRQRDVSIELANIIA
                     TKVLNSENILRLIFKGKGGDLLVKLVMDNAFKGVEKLMKDKAPMVPLILGSDKTRKIK
                     EKVADNIISILPEAANKIQTYITDSLKIESTVAERLTQLSTVQFEDILHSVFKEDEMT
                     LILLGALLGGLVGMAQAAIVVEPSQLPAFLRF"
BASE COUNT          630 a          516 c          485 g          656 t
ORIGIN      
        1 agtctgcgaa tccaggtccg gaagttccgg tcagaagttt ctgaagatta gccattccga
       61 gggtcagagg aaaatgatca tagataaatg caatggctcc tccgtccgca agtttggttc
      121 tgtcctgcca gaagagtccg aggaatgcaa agttgatgat ctgaaaaaag aatcctaccc
      181 gtttgaaaat cagtgtaatt cctgcgaata tataagaaag tccaaatatt cctattccca
      241 taatcaaaag aagcagtatc gtctgaggga tgtgaggaga gaggctcagg ctgatatttt
      301 caatgctgaa agtgtatgct ccgaggagca catagaaact caacatctgc ctcggcatct
      361 gagaaagacc tctggcaagc agaacctcac cgagagtatg accggatatg gacagctgtt
      421 cgagggttcc tgtctgagat tcattctgta tctgtcccga ccagcccatc tgagtcgcca
      481 ttacgaactg catcagtgaa tatccgagga tcatcttgtc cttgttcagt actccgctcg
      541 cacttcctgc aagctgatga aacccgaaca gaattgcgag aaggatcagg tacataaaga
      601 acagaaacga aattgtttct gcagggtatc ttttcataaa gataaaatct cttacagtct
      661 cggcttttaa tatcagtgaa aaatcaatca gttttctcat acttcatcct caaattttgc
      721 ggtaattttt acaggtccga atgtgcctgt ttcttttaca tccagatgcg gatatgcttc
      781 cgcatacttg tccatcattt cctgataggg cagatcatcc cttgttccga aatcctgcag
      841 gtattccatg tgctttttgc cttgaagatc aagcggatgg aaaagagaat tgttttctcc
      901 gtcaaactcc agagtgaaaa tcccggctct gtcgcagaga gacttattga atgcaggagt
      961 gcatttaacc catttgcctt cgatgaacat ctccgtatat ccatgatata caaaaatatc
     1021 tgttttcatt aaggaggaga ggcggggcga gctgatatga ttccttacat tcgcaaatcc
     1081 tatcttggag ggaatacctg cgagtctgga cactgcagca agaagaagcg ctttttcaac
     1141 gcagaatccg tatcttcttt ttacgatttc cgatgcgcgg agaaattcag gtttcagcag
     1201 aatatgataa ggatcataac gaatttcatc acggacctta taatacagtg ccttggcttt
     1261 ttccttttca gattttatgg aaaggatgtc ctctgtgacg aaatcccgga cagatctgtc
     1321 caggaaattc atactgtatg tttcctgaag agtgttcaaa tcagaaacgc aggaaagcag
     1381 gaagctggct tggttctaca acaatggctg cctgtgccat accgactagg cctccgagga
     1441 gcgcaccgag caggatgaga gtcatttcat cttccttgaa tacggaatgc agaatgtctt
     1501 caaactgaac agtggaaagc tgtgtcagtc tttctgctac tgtggattct attttcagac
     1561 tgtcggtaat gtacgtctgg attttgtttg ctgcttccgg aagaatgctt atgatattgt
     1621 cagccacttt ttccttgatc tttctggtct tgtcggaacc gagaatcaga ggaaccatag
     1681 gtgccttgtc cttcatcagt ttttccacac ccttgaatgc attgtccatc accagtttca
     1741 ccagaaggtc tccgcctttc cctttaaaaa tcagcctaag gatattttct gaattcagaa
     1801 cttttgttgc aataatattt gcaagttcta tggaaacgtc tctctgtctt ctgagaaaca
     1861 gtccctggta tttgaagaat ccgaaatttg tcacttccaa tggccggaag atcatctgca
     1921 gagcgagcca gtttgtaaca tatcctacaa tcactcccat aatcggcatc gtccaccact
     1981 gaccaaggat ggaaaggaat gccagctgaa tgagtccgat caggaatccg aagtaaatcc
     2041 ccgaaactac aaggaatcga aactcaggtc cgccgcatct tttgaaaagt tcaatcagaa
     2101 ctcctacatt ttttccgcag agggaatcct tgaccacttc atcgaattcc aataccgaat
     2161 ctaaatcgtc tccgaaggat ttgtagactt ccacaatctg ctttgggatg acttcaccga
     2221 tctgcttttc tattccctgt tttgcaaaat cgggaagaat tttccagatt tcagggtttt
     2281 cgccttc
//
LOCUS       DAOQGJ010000497         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_30941_length_2286_cov_3.432990, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000497 DAOQGJ010000000
VERSION     DAOQGJ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30941_length_2286_cov_3.432990"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1873)
                     /locus_tag="PK683_08765"
     CDS             complement(<1..1873)
                     /locus_tag="PK683_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH08581.1"
                     /translation="MEFSYILLSANFFDRFAPAVLTFLISLFLFIISRKERTALYLGF
                     TFAALTVFNVGYLLGYGLDHPAGAAGWHIACVIVLVVLFKIQFVYYLPEKLNKYEPRI
                     VLWTGLLIYLFIQVQYTLSANAAGYRFNFKNQIYASEYISPYVPILTFVLFIWLFAVQ
                     IRQMLKFTKNDHTERSILLRFFFRVFLPVSREGRLIRSFLILTFFELTVNITVAAGFL
                     TSFVSHIVMNLMMSSGLMLIYLAYTVVYVNSSRKNVTFMIKIAGVALVFLLVVSSFIG
                     SRSISRTEESYDRENLSLIKGIISNPDWQKNSMYADNLEYAVLRKNTMGMYSQVYRVI
                     WHTKGKINNELLLSSDESDRQFNLRNLVRKIQNQNRGISAAEAEKRSIDRLNETVLSE
                     NQRYYRMAGKERYIYYIIKSDRDSFMEFGFSYREYRKRIHDTVIIETLTLGFLVFLLL
                     TVFPLFFRINLVLPLRRLLEGVKEINEGNLKVHVKVDVYDEIGFLTNSFNNMVSSIES
                     AQNQLEDYAKNLEIKVDERTRQLQDSLNKINDLKTKQDADYFLSSLLVGPLSHPGFST
                     PSVRVDFKIKQKKEFEFKKKKHEIGGDYCTTVRLRLQNRDCTVILNADAMGKSLQGNG
                     "
     gene            complement(2038..>2286)
                     /locus_tag="PK683_08770"
     CDS             complement(2038..>2286)
                     /locus_tag="PK683_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08582.1"
                     /translation="AAENLIPYQDKKNEKCIEKIADEPFETAKRELNAFEILITLGTL
                     GFSEILRNVEENNSRERFAVRQKAVKEHLSFLSRMKGN"
BASE COUNT          699 a          486 c          411 g          690 t
ORIGIN      
        1 ccccgtttcc ctgcagagac ttgcccattg catccgcatt cagaatgacg gtgcagtctc
       61 tgttctgaag gcgcagacgc actgttgtac aatagtcccc gccaatctca tgtttcttct
      121 ttttaaactc aaattccttt ttctgcttta tcttaaaatc tacacggacg gacggtgtgg
      181 aaaatccggg atgagaaaga ggacccacga ggagactgga aagaaaataa tctgcatcct
      241 gttttgtctt cagatcgttg atcttattca gactgtcctg aagctgtctg gttctctcat
      301 ccactttgat ttccagattc tttgcataat cttccagctg attctgagcg gattcaatgg
      361 agcttaccat attattgaat gaatttgtaa gaaaacctat ctcgtcataa acatccacct
      421 tcacgtggac tttcagattt ccttcattaa tttccttgac cccttcaagg agtctccgaa
      481 gaggcagcac aagattgatt ctgaagaaga gaggaaacac agtcagcaga agaaatacca
      541 gaaagcccag agtcagtgtc tctataataa ctgtatcatg aattctcttt ctgtattctc
      601 tgtaactgaa tccgaattcc atgaaagaat ctctatctga ttttataata taataaatat
      661 atctttcttt tcctgccatt ctgtaatatc tctgattttc tgacagaaca gtttcattaa
      721 gtctgtcgat agaccttttc tccgcctctg ctgcggaaat ccccctgttc tgattctgaa
      781 tttttctgac aagatttctc agattgaact gcctgtctga ttcatcactt gacagaagaa
      841 gctcattgtt aatcttccct ttcgtatgcc agataactct gtaaacctga ctgtacatcc
      901 ccatcgtgtt tttgcggagc actgcatatt ccagattatc agcatacata gaatttttct
      961 gccagtccgg attggaaata attcctttaa tcagtgaaag attttcccgg tcataacttt
     1021 cttccgttct ggaaatggac ctggatccga taaaactgga aactaccaga agaaaaacaa
     1081 gagcgactcc tgcaatcttg atcataaagg tgacattttt ccggctgctg ttcacatata
     1141 ctactgtgta ggcaagatag atcagcataa gtcctgaact catcataagg ttcattacaa
     1201 tatgtgaaac aaagctggtc agaaagcctg cagcgacagt aatattcact gtaagttcaa
     1261 agaaagtcag tatcagaaaa gatctgatca gtctcccttc tctggaaacg ggcagaaaga
     1321 ctctgaaaaa aaaccgaagc agaatacttc tttcagtatg atcattcttt gtgaatttca
     1381 gcatctgccg gatctgaacg gcaaagagcc agataaaaag cacaaaggtc aggataggaa
     1441 catacgggga gatatattcc gaagcgtaaa tctgattttt aaaattgaat ctgtaacctg
     1501 ctgcatttgc actcaaagta tactgcacct gaatgaataa ataaatcaga agtccggtcc
     1561 agagaacaat tctaggttca tacttattca gtttttccgg aaggtaatag acaaactgaa
     1621 ttttaaacag tacaaccagg acgatgacac aggcaatatg ccagccagca gccccagccg
     1681 gatgatcaag tccataaccg agaagatatc ctacattgaa tacagtcaag gcagcaaagg
     1741 tgaatccaag ataaagtgct gttcgctcct ttctgctgat aatgaacagg aaaagactga
     1801 ttaaaaatgt aagcacagca ggggcaaatc tgtcaaaaaa atttgcactc agcaaaatat
     1861 aggaaaattc cataattttt attagagtat cctttgtata atatcagatc atctgatgtg
     1921 aagattagat tgtatgattc cgcgattatt gaattttgaa cagtaaaaat ttctattcaa
     1981 gattaagatc ctgtctcaaa ataaaaagat ttttaccgat attgaggaac agacaattca
     2041 gttccccttc attcgactca gaaaagacag atgctccttg accgccttct gtctcactgc
     2101 aaacctttcc ctggaattgt tttcttccac gtttctgagt atttcagaaa aacctaatgt
     2161 tcccagagta atcagtattt caaaggcgtt cagttctctc tttgcagttt caaacggttc
     2221 atctgcaatt ttttcaatgc atttttcatt ttttttatcc tgatagggaa tcagattttc
     2281 cgctgc
//
LOCUS       DAOQGJ010000498         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31002_length_2283_cov_3.400808, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000498 DAOQGJ010000000
VERSION     DAOQGJ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31002_length_2283_cov_3.400808"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2283
                     /locus_tag="PK683_08775"
     CDS             <1..>2283
                     /locus_tag="PK683_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04388 family protein"
                     /protein_id="HNH08583.1"
                     /translation="IGTPVQSERNYILHYNTYQTYRDRLNGPGELMYEFISKMYSELK
                     KPPEERSLTAAAEAVNLYLAEQKKETDKMAAFWESKIYTDINLGTDSTYRFVYNTFKE
                     ADYFRHSFVKDIEILYRLQENLRKLDVFSSGSVTLAYGFSEQNMDAVRGNMPGIYDSS
                     IQPLYADSVKMPYLRANNLKTYSFAEKDTAVLENSMELSSVMERIKSSLKMIHPADNI
                     EIDYIDPQMNLHASSVNLFSDWYVAGHDKVGGNLRTDGLNYRRYRTNFLIDGFAEAGG
                     FDYRQDSEENIWVEIKYRIRDKNAEANWIQWANFQTDVNSQTVLWGSQLNAVQNWTVQ
                     SANFRNRASLWNQEKQSLQNEIQNNYNKSMEDLMNEKSAWLADMDSVRSKAVSGDFSA
                     DVPSVPLLSYENRISTIRDKTAGFENMQTPDFSSFENFSSVLDDTMNGFLNLLTAKSI
                     TDSALSTSSSALNSMAAMLSSQRTMTDAYEVPPENRKEYYDAVKNFSAGGICAGDGFE
                     KNRQICEGIYNGPFFKRQYAGVEVRDGQIEVTVMEKTGRVFLKEGGDAKDQDDYIQET
                     VKKTYYINTPGEAKIADMSSLGSVFKGSWSSKTEGWQNTERKEFGSDLAKYWQYWTDK
                     PQQNLEETAETGSVQEQKKTLLSSVYDEVSKNSSIYLNHFYTNTGTAEKPVYSYNKEG
                     LFSKMAALMSSRESENSQHFSNLARDNAKQEASKAEMLESLATAVVTGGLSQRNLSSW
                     LKDRMTQAVKGKWAQTAADA"
BASE COUNT          759 a          436 c          533 g          555 t
ORIGIN      
        1 gataggcaca cctgttcagt cggaaaggaa ttatatcctt cattataata cataccagac
       61 ttacagggac aggctgaacg gaccggggga actgatgtat gaatttatca gtaaaatgta
      121 ttctgaactg aaaaaacctc ctgaagagcg ttcactgact gcagcggcgg aggcagtcaa
      181 tctttatctt gcagaacaga agaaagagac cgataaaatg gctgctttct gggaatccaa
      241 aatctacaca gacatcaatc tgggaactga cagcacatac agatttgtct acaatacatt
      301 caaggaagcc gactacttca gacactcttt cgtaaaggac attgaaattc tttacagact
      361 tcaggaaaac ttgagaaaac ttgatgtttt cagttcaggc agcgtcactc ttgcatacgg
      421 attttctgaa cagaatatgg atgcagtaag aggaaatatg cctggaattt atgacagttc
      481 cattcagcct ctctacgcag attcagtaaa gatgccgtat ctgcgtgcaa ataatctgaa
      541 gacttattcc ttcgcagaaa aagacactgc tgttctggaa aattccatgg aactcagttc
      601 cgttatggaa agaatcaagt cttcactcaa gatgatccat cccgcagaca atattgaaat
      661 agattatata gatccgcaaa tgaatctcca tgcctcttca gtgaatctct tttcggactg
      721 gtatgtggcg ggtcatgaca aggttggcgg aaatctgaga acagacggcc tgaattacag
      781 aagatacaga acaaattttc tgatagacgg gtttgcggaa gcaggaggct ttgactaccg
      841 tcaggactcg gaggaaaaca tctgggtgga aataaaatac aggatcaggg ataaaaatgc
      901 ggaagccaac tggattcagt gggcaaattt tcagactgat gtaaacagtc agactgtcct
      961 ttggggttca cagctgaatg cagtacagaa ctggactgtt cagtctgcaa atttcagaaa
     1021 cagggcatcc ctctggaatc aggaaaagca gagtctgcag aatgaaatac agaataatta
     1081 taataaaagc atggaagatc tgatgaacga aaaatctgcc tggcttgccg atatggactc
     1141 cgtccggtcc aaggcagttt caggagattt cagtgcagac gttccctctg tccctcttct
     1201 gtcttatgaa aacagaatca gcacaatccg ggataagacg gcaggttttg aaaatatgca
     1261 gactcccgac ttctcttcat ttgaaaattt ttcatctgtt ctcgatgata caatgaacgg
     1321 tttcctgaat ctcctgactg caaaaagcat taccgattcc gccctaagca cttcttcgtc
     1381 agcattgaac agcatggctg caatgctttc ttcacagaga acaatgactg atgcctatga
     1441 agttccgccg gaaaacagaa aggaatatta tgacgctgtg aaaaattttt ctgcgggagg
     1501 gatctgtgct ggagacggat ttgaaaaaaa tcggcagatc tgcgaaggga tttacaacgg
     1561 tcctttcttt aaaagacagt atgcaggagt ggaggtcagg gacggacaga tagaagtgac
     1621 tgtaatggaa aaaactggaa gagtgtttct gaaggaaggc ggagatgcaa aggatcagga
     1681 tgattatata caggaaacag taaaaaaaac atattatata aatactccag gagaggccaa
     1741 aattgcagac atgagcagtc ttggaagcgt atttaaaggg agctggtcct ccaaaacgga
     1801 aggatggcag aatacggaaa gaaaggaatt cggttcagat cttgcaaaat actggcagta
     1861 ttggacagac aaacctcagc agaatctgga agagacagca gaaactggat cggttcagga
     1921 gcaaaagaaa acacttcttt catctgttta tgatgaggtg agtaaaaatt catcaatata
     1981 tctgaatcat ttttatacaa acaccggaac ggcagaaaaa cctgtctact cttataataa
     2041 agaaggtttg ttttcaaaaa tggcagcttt gatgagttca agggaatcag aaaattctca
     2101 gcatttttca aatctggcga gggataatgc aaaacaggaa gcatcaaagg cagagatgct
     2161 ggagagtctc gcaactgctg tcgtgacagg aggtctctct caaagaaatc tttcctcatg
     2221 gctcaaggac agaatgactc aggcagtaaa aggcaaatgg gcacagacgg cagcggatgc
     2281 aat
//
LOCUS       DAOQGJ010000499         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31007_length_2283_cov_2.779174, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000499 DAOQGJ010000000
VERSION     DAOQGJ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31007_length_2283_cov_2.779174"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            598..828
                     /locus_tag="PK683_08780"
     CDS             598..828
                     /locus_tag="PK683_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08584.1"
                     /translation="MYKISLFLILILGCKNFDVQLAPKFNESKVVKLKVPIRQERASG
                     YHEDEDISSLCEPKKMVYIKFSFNQPQEVWCE"
     gene            1331..2059
                     /locus_tag="PK683_08785"
     CDS             1331..2059
                     /locus_tag="PK683_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002556595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08585.1"
                     /translation="MNFKTKLFCLAAGSVFFFSSSVSALGTYAEGWATAKILQFESRG
                     IIFTSYEGSLELTTFNKEEKCDEAKDECFAPVKQKLDFSVRPENSEVVNFLNKHVNQE
                     VLIQYNIHRITAIALSSQFEVLKAQKQETSAPAGADKLVIKPTGSKRNFSVSGKIVQL
                     DYQGTLIGTYEGLYLDETRGKIHPFSITSEDMAKYAWNCMKANEKYNMGISVAFVTGL
                     RKSNYDLFEINYKAPAGGVVPPVN"
     gene            2067..2270
                     /locus_tag="PK683_08790"
     CDS             2067..2270
                     /locus_tag="PK683_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08586.1"
                     /translation="MNKDLLDQKINLLYSYSEVDPAVTERIKESVLSGDDWLLRRINP
                     YRFAESCSLDSAKTVMPFFMHQE"
BASE COUNT          704 a          407 c          450 g          722 t
ORIGIN      
        1 cagagaactg cgcagtgctt tggtaatttc tatatttgta agaagattat tcttatcagc
       61 tccttcgacg aaatctacat aaattgattc tccaagcggt tgagcggctt tgatttcttt
      121 tgtggccata tattcgtaca ttgcgcttcg aattccggta gcacaattca gaagagggga
      181 aaccaaaaca gagattatac aaagggcaat atttcttttc ataagaagtt tatcttcata
      241 tatgtttttt ctgtaaagaa aaaatgttct tgcagaaaaa taaccaatga attattcctt
      301 ttctatgctg ttaattttta agaaagtttt cttagtcctg atattgtcct ttttgttcag
      361 ttcatgtttt agtcataaat acattattac taaaagcaat acggtgagcg aggcagagga
      421 aattcatatt cgaatgttca cacttttctg gataattccg atttataatg atatcaatcc
      481 tgatactgta tgcctaggaa aaaggattcg ctctatcaat atgtatgaca gtgcttttac
      541 tggctttgtc tgcggaatta ctgcatcttt gatatgtcct gcagcagtag gtatagaatg
      601 tataaaataa gcctttttct gattttgatt ttgggatgca aaaattttga cgttcagctt
      661 gctccaaaat ttaatgagtc aaaagtagtt aagctgaagg tgcctattcg acaggagaga
      721 gcatccggct atcatgaaga tgaagatata tcttccttgt gcgaacctaa aaaaatggta
      781 tatataaaat tcagtttcaa ccaaccgcag gaagtatggt gcgaataata acagcacata
      841 ctgatatttt ttttagtgct gcttgttggt tttcctgatt attagatttt tacggtaact
      901 ttgaaataat ggactcaatt cctgtttcag ggagtaatat gcgcttcaag tcaaaaaaac
      961 ttttcataag tctgctcagg aaatggctat cagccttatt gcttgtttaa taactcatct
     1021 tacgcagttt aggcgttttg ggcaggaacg gctgccgacg gcttgaggac aaatgaaggc
     1081 atactgaaat caaagttctt tgagtcggtt gaatgcagca aataaccttg tgaacaagtg
     1141 attttgagat ctttaaatga aaaaatagat ccttgattag atctctgcca ttttaataat
     1201 catatgttcc agctggcaaa tttgtttcag cacagttaaa aactgcttgc agtgaaaaaa
     1261 gattctgatg aactttcctt tacattcggt gcagatgtat cggatgaata cttaaccctg
     1321 gagttattta atgaatttca aaacaaaact tttctgtctc gcggcaggtt ctgttttctt
     1381 tttttcctct tctgtatctg cactcggcac ttatgcagaa ggctgggcaa ctgcaaagat
     1441 tcttcagttt gaatccagag gaatcatatt cacttcctat gaaggttccc ttgaactgac
     1501 cacattcaat aaagaagaaa aatgtgatga agccaaagat gaatgttttg ctccagtaaa
     1561 gcagaaactt gatttcagcg tgagaccaga aaattcagaa gtcgtcaatt tcctgaacaa
     1621 acacgtgaat caggaagtcc tcattcagta taatattcac agaattactg caattgcact
     1681 gtcctctcag tttgaagttc tgaaggcaca gaaacaggaa acgtctgctc ccgcaggtgc
     1741 agacaaactc gtcattaaac cgaccggctc gaaaagaaat ttttccgttt caggaaaaat
     1801 tgtacagctg gattatcagg gaacactgat tggaacatac gaaggactgt atctggacga
     1861 gaccagaggg aagatacatc cgttctcaat cacaagtgaa gatatggcaa agtatgcatg
     1921 gaactgtatg aaggccaatg aaaagtacaa tatgggaatt tcagttgcat tcgtgacagg
     1981 gctgcgcaaa tctaattacg atctgtttga aattaattat aaagcacctg caggcggagt
     2041 cgttccaccg gtaaactgat ttttttatga ataaagatct tctcgaccag aaaatcaatc
     2101 ttctgtattc ttattccgaa gtcgatcctg ccgttacgga aaggataaag gaatcagttc
     2161 tttcaggtga tgactggctt ttgagaagaa tcaacccata caggtttgca gaatcctgtt
     2221 ctctggattc tgcaaagact gtaatgcctt tctttatgca tcaagaatag gcctctttga
     2281 cct
//
LOCUS       DAOQGJ010000500         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31011_length_2283_cov_2.237433, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000500 DAOQGJ010000000
VERSION     DAOQGJ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31011_length_2283_cov_2.237433"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK683_08795"
     CDS             <1..180
                     /locus_tag="PK683_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08587.1"
                     /translation="RLKDALTVLEELKIKVASIDERENYRAPVYFELLQDFKVLLRET
                     LIGLGRKGEADEIHL"
     gene            177..1301
                     /locus_tag="PK683_08800"
     CDS             177..1301
                     /locus_tag="PK683_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH08588.1"
                     /translation="MISQNICYFDYNATHPPFADILSTACIDYSIHYYNPSGAGRFSL
                     QRQGLIEETRKYFAGLFGKKEEGIVFCSTGTEANLLILGCLKFSQYNAQTVYVSGLEH
                     SSIYGAFEILGMDYEMIPLSRRGEIDLDFLDRKMKISPRPVVLIGAANESGIIPDLKS
                     VSVICRREGMPLISDQMQAFGKINIEYPNLDAFSCSGHKIGAGIGASIAYIPPEWKTG
                     YRIFKGGNQENGFRAGTENSAAIYSFRMASERQHSMLPGKNDRLLAFRERIENTLSEF
                     GSEIVGSDISRLPGTCFAILKSEDIDFLLIGLEEKGIVISTGSSCKSRSREPALSLLN
                     MGYSKEEALRAVRISFGIFNSEDDLEYLLENLRSLLTILV"
     gene            complement(1260..1427)
                     /locus_tag="PK683_08805"
     CDS             complement(1260..1427)
                     /locus_tag="PK683_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08589.1"
                     /translation="MESRKTNLISMQTVRLMHSLQKDGISAAVTEESEMLESRRNRSD
                     KDRQEGPEILK"
     gene            complement(1467..>2283)
                     /locus_tag="PK683_08810"
     CDS             complement(1467..>2283)
                     /locus_tag="PK683_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004474960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH08590.1"
                     /translation="TKNLLWRFLGTDKPLQELNSTLNKEKVYNVLVEQDFKDKSVKDQ
                     IKALSDSDAAGTGGLTEKEGFHSLSPFYEFLMGSKGSAASVPNETASKNKKDDDVYEV
                     GIYKSEPVSKPVVKAPPSSTVSQPNPSQQTKIPANYRFQQDFLFRWDGSQALSIPRKQ
                     LAGYHYFKSMLRKIENSFYPPGGGNFAYRDSRGYVVREGIVPGEVKVMFLLNDMGDVI
                     DIKLVSSMGQKIVDTACLDSIRGQNFGAVPTEVKENGMTFGINFIFPEFLNYR"
BASE COUNT          609 a          482 c          508 g          684 t
ORIGIN      
        1 agactgaagg atgcgcttac cgttcttgaa gaactgaaga tcaaggtggc aagcatagat
       61 gaaagggaga attacagagc acctgtttat ttcgaacttc ttcaggattt caaggttcta
      121 ttgagagaaa cactgatcgg tcttgggagg aagggagaag cagacgagat tcatttatga
      181 tatctcaaaa catctgctat tttgactaca atgcaactca tcctcctttt gcagatatcc
      241 tgagcactgc ctgcattgat tattccattc attattacaa tccgtccgga gcaggacggt
      301 tttcactgca aagacagggg ctgatagagg aaaccagaaa gtattttgcc ggactcttcg
      361 gtaaaaagga agagggaatt gtattctgtt ctacaggaac cgaggccaat cttctgattc
      421 tcggatgtct gaaattctct cagtacaacg cacagactgt atatgtctcc ggactggaac
      481 attccagcat atacggtgct tttgaaatac ttggaatgga ttatgaaatg attcctctca
      541 gcagacgcgg tgagattgat ctggattttc ttgacagaaa gatgaaaatt tcacccagac
      601 ctgtggtgct gatcggtgct gccaatgaat caggcatcat tcctgacttg aagtctgtct
      661 cagtgatctg cagaagggaa ggaatgcctc tgatttcaga tcagatgcag gcattcggaa
      721 aaatcaatat agaatatccc aatttagatg ctttttcatg cagcggtcac aagatcggtg
      781 caggcatcgg tgccagcatt gcttatattc cgcctgaatg gaagacagga tacagaatat
      841 tcaaaggtgg aaatcaggaa aacggtttta gggcaggcac cgagaattct gccgccattt
      901 attcattccg tatggcttca gaaagacagc actcaatgct tcctggaaaa aatgacagac
      961 ttcttgcttt ccgtgaaagg attgaaaaca cactttctga gttcggatct gaaattgtag
     1021 gttcagacat cagtcgtctt ccgggaacat gttttgcaat tcttaagtca gaagacattg
     1081 attttcttct gatcggtctt gaagaaaagg gaatcgtcat atctacaggt tcttcctgca
     1141 aatccaggtc acgggaaccg gctttgtcac ttttaaatat gggttattca aaagaagagg
     1201 cactgagagc agtgcgaatt tctttcggga tcttcaattc agaagatgat ttggagtatt
     1261 tacttgagaa tctcaggtcc ctcctgacga tccttgtctg atctgtttct tcttgattcg
     1321 agcatttcgc tttcttccgt gacagcagcg ctgattccgt ctttctgaag agaatgcata
     1381 agccttactg tctgcatcga tattaaattt gtttttctgc tttccattct agatgtactt
     1441 tcggtttcca ggatggtgta tcttgattat ctgtaattta aaaattcagg aaaaataaaa
     1501 ttgattccga atgtcattcc attttctttg acttcggtcg gaacggcacc gaaattctga
     1561 cctcggatgg agtcaaggca ggcggtatct acaattttct gtcccatgga agatactagc
     1621 ttaatgtcta tgacatctcc catatcattg agaagaaaca tcactttgac ctcacctggc
     1681 acaatgcctt ccctgacgac ataacctctg ctgtccctgt aggcaaagtt tcctccgcct
     1741 ggcggataaa aactgttttc aatttttcga agcatacttt tgaaataatg atagcctgca
     1801 agctgctttc tgggaatgga gagggcctga gagccgtccc atctgaacag aaaatcctgc
     1861 tgaaatctgt aattggctgg aatctttgtc tgctggctgg gattgggctg agaaacggta
     1921 ctgctgggcg gtgccttgac tacaggtttg ctgactggct ctgacttata aatgcctacc
     1981 tcatacacat cgtcatcttt cttgtttttt gatgccgttt catttgggac tgaagcagcc
     2041 gatccctttg atcccatcaa aaattcataa aatggactta aggaatgaaa accctctttt
     2101 tcagtcagtc cgccggttcc tgccgcatct gaatcggaaa gtgccttgat ctgatccttg
     2161 actgacttgt ccttaaaatc ctgctctaca agcacattgt agaccttttc cttgttcagg
     2221 gtgctgttca gttcctggag aggcttgtct gtgcctaaaa aacgccataa caggttcttg
     2281 gtg
//
LOCUS       DAOQGJ010000501         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31068_length_2280_cov_3.211685, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000501 DAOQGJ010000000
VERSION     DAOQGJ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31068_length_2280_cov_3.211685"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PK683_08815"
     CDS             <1..757
                     /locus_tag="PK683_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH08591.1"
                     /translation="NCESELVKLPDQSSFYLNDEYAKESGITIPDTIVERAKLTRLFG
                     LGVNLINEGKYNSAKTVFESILKKDKENKSASSFYQLVLEKLSGSKVRDLLASAESHM
                     KNKRFSQARSDYQRILSINPNILEAKMGVQNSLQLQSENERMTGQSHARSGKPFEAVR
                     MFQTALKTLPTNAAATNDLNALRSAESHNMPEYVKKGVKLYNDREYEKAIEVFDNALL
                     IVPNNKEAIEYLRLSKKKKDALITLKGKLEKDR"
     gene            1031..>2280
                     /locus_tag="PK683_08820"
     CDS             1031..>2280
                     /locus_tag="PK683_08820"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH08592.1"
                     /translation="MEQIRLALEHPDFFPHFVITGMDGLGKMSGILSLLQKEYSAKRL
                     KEKLYLDRMSKKVVPESLVSEEVLNAETLFEPLFDIHCKGTPIVYDPNPSIMSLVGIP
                     SKDSYHPGSLVKAFGGFLILPLTRMTSEPAVFDVLRSCLITEGIDFMNLPELLFFQKY
                     DRTLPRLPISTRVILVGEEHHFDQLIKKDSTVIELFKMRADLEYEAVLNEKNAGKFSF
                     LIDSWGRESFPKFDSSAKKRLAEESLIQNESKTKISLNLADLKFVYDEAVVLGVRKKA
                     KLIKAADVDAAIKNLDFRFTQIKRKYYEDLKDGIYNLTVKGKRLGRINGLSVFSPYSS
                     SQEYGQVNVISARAVMGTGNIINVEREVNLSGDSHDKGVFILQSFLKGMFANFASLGA
                     DISIVFEQNHSIIDGDSASTAELMA"
BASE COUNT          711 a          435 c          505 g          629 t
ORIGIN      
        1 caactgtgaa tctgaattgg tgaaacttcc ggaccaatcc tcattttatc tgaacgatga
       61 gtatgcaaaa gagtccggaa ttactattcc ggatactatc gtcgaaagag cgaagcttac
      121 cagacttttc ggactgggag tcaacctgat caacgaaggg aaatacaatt cagccaagac
      181 agtatttgaa tctattctga aaaaagataa agaaaacaag tcggcctcct cattttatca
      241 gcttgttttg gaaaaactgt ccggttcaaa agtaagagac cttttggcaa gtgctgaatc
      301 gcatatgaaa aataagagat tttctcaggc cagatccgac taccagagaa tcctttccat
      361 caacccgaat attctggaag cgaaaatggg agtgcaaaac agtctgcagc tgcaaagtga
      421 aaacgaaagg atgactgggc aaagccatgc cagaagcgga aaacccttcg aagctgtcag
      481 aatgttccag actgcactta agactcttcc tacgaatgcg gctgcaacga acgatctgaa
      541 tgcactcaga agtgcggaat cgcataatat gccggaatat gtcaaaaaag gcgtcaaatt
      601 gtacaatgac agagagtatg agaaagcaat agaagttttc gacaatgctt tattgattgt
      661 tccgaacaat aaggaagcca ttgagtatct gcgtctttcc aaaaagaaga aggatgcatt
      721 gattacactc aagggaaaat tggagaaaga caggtagttt cggtattttg agcatcagtc
      781 catcaaggct tttcagtatt tgatgacatt ttgcctaaat caaagacttt cattcatcta
      841 gacgacagaa ttgctgccct tagacaggta tatatatgct tgtctttgca gtcctctctg
      901 atactttggt ttcggaatgc tgaaaaagct ctcacatcaa gatattaccg tagaaattcc
      961 ggagcatttt tttaagacaa aacctgctca tcaaaaaaac tccgaatttt ttattttcca
     1021 gccgaaggca ttggagcaga ttcgacttgc attggaacat ccggattttt tccctcattt
     1081 tgtaattacc gggatggatg gactcggaaa aatgtccggt attttatcac ttctgcagaa
     1141 agagtattct gcaaaaaggt taaaagagaa attatatttg gacaggatgt ccaagaaagt
     1201 agttcctgaa tctttggttt cagaagaggt attgaacgcg gaaactctgt ttgaaccgtt
     1261 gtttgatatt cactgcaaag gaactccaat cgtttacgat ccgaatccca gcattatgag
     1321 tcttgtgggg attccttcaa aagacagtta tcatccagga agccttgtga aggcttttgg
     1381 cggttttctg attctgcctc tgaccagaat gacatccgaa ccggcagttt ttgatgttct
     1441 cagatcgtgc ctgattactg aaggcatcga ttttatgaat cttccggaac ttctgttttt
     1501 tcagaaatac gacaggaccc ttccgcgact gcctatttcg actagagtaa ttctggtagg
     1561 cgaggagcat cattttgatc agctgattaa aaaagacagt acagttatcg aactttttaa
     1621 gatgagagct gacttggagt atgaggctgt tctgaatgaa aagaatgcag ggaaattttc
     1681 atttctgata gacagctggg gcagggagag ttttccaaaa tttgacagtt cagcaaagaa
     1741 aaggctggca gaggaatccc tcatccagaa cgaaagcaag actaagatct ccctgaatct
     1801 tgctgactta aaatttgtct atgatgaagc agttgtactg ggagtcagaa aaaaagcaaa
     1861 attaatcaag gcagcagatg tagatgccgc catcaaaaat ctcgatttcc gattcactca
     1921 gatcaaaagg aaatactatg aggatctgaa ggatggaatt tataatctga cagtcaaggg
     1981 aaaacggtta ggaagaatca acgggctctc cgttttctcg ccgtattcat ccagtcagga
     2041 atatggacag gtcaatgtca tttctgcacg cgcggtgatg gggacaggaa atattatcaa
     2101 tgtggaaaga gaagtcaatc tttccggcga ctctcatgat aagggggtat ttattctaca
     2161 gtctttttta aagggtatgt ttgccaattt tgcctcactt ggtgcagata tttccattgt
     2221 atttgaacag aaccattcca ttatcgatgg agattctgcg tccacggccg aactgatggc
//
LOCUS       DAOQGJ010000502         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31080_length_2279_cov_3.766187, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000502 DAOQGJ010000000
VERSION     DAOQGJ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31080_length_2279_cov_3.766187"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2279
                     /locus_tag="PK683_08825"
     CDS             <1..>2279
                     /locus_tag="PK683_08825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH08593.1"
                     /translation="MTAINAPFFHRVLQFLWAATFFSLPVFYIWGYQTANNYANILVF
                     AAGFTAFFCAVYLSFKRIRQAYFFLIAFSLVITSGLVLLATFLGFFELNFFILNSYQA
                     AQALEAALMGFALADRINTVTIEKEIAYKNSYENQKIALENLEKFNEMKNNLFAGISH
                     EILTPLNGIIGIAESLRSGSEGTLSANVMQNLHLIMSNGKRLSNMVGNLFDLSNLFNN
                     NIVLETSVFNLNDFVYKISEAYSAIAKRKNVQLVLNIPDLLFTEADKNRIFQVFNNLI
                     SNALKSIAQGEVRVTAKRYQEDNGKILVTVSDTGPGLPKEIRELVFNDYSDFTPLEKG
                     KSNGFGFSMILTKKIIELHGCDIWMDAKSDKGSVFYFTMPAAKDVPASAEKEKKIVSE
                     ESSQKKQEQDKQATTVLIVDDEPINLYVLESHLEKEGYKVLQAVNGYEAQDICNSEKR
                     PDILLLDVMMPGIDGFEVVRNLRKKYSLHELPIILLTAKNTMLDVQEGFDAGANDFLN
                     KPFDRIELLSRVNTLITLKKSVSENQKHITIQHELAIARKLQKSLLPASKPDMAGIEM
                     EARYIPMTYLGGDYYDYHISRENQLGVIIADVTGHGVAAAMIASMLKTAFQVQRQNFS
                     RPAYVLEQINKIMYGNELLTATYIVIDLNENILRVANAGHPHLYVAKKDGQIISLNPK
                     GKLIGFFPEIKCEEIWHPIETGDRIILYTDGLVEASDIFGEPLGDERFTEFIQQNVQL
                     DSKEFISSALGFVFAWSGK"
BASE COUNT          694 a          445 c          503 g          637 t
ORIGIN      
        1 gatgacggca atcaatgcac cgttctttca tcgagtactt cagttccttt gggcggcgac
       61 atttttttcc cttccggttt tttacatttg gggatatcag actgcaaaca attatgcaaa
      121 tattctggtg tttgctgccg gatttacagc cttcttttgt gcagtttacc tctcattcaa
      181 aaggatacgt caggcctatt ttttcctgat agcattttca ctggtcatca catccggact
      241 tgttctcctg gcaacctttc tcggtttttt tgagcttaat tttttcattc tgaactccta
      301 tcaggcagca caggctctgg aggctgcatt gatgggtttt gcgcttgctg acagaatcaa
      361 tacggtcacg attgaaaagg aaattgctta taaaaattca tatgaaaatc agaaaattgc
      421 tctggagaat ctggagaaat tcaatgagat gaagaataat ctttttgcgg gcatcagtca
      481 tgagatactc acacctctga acggtatcat tggaatagcc gaatctctca gatccggttc
      541 ggaaggaact ctttcggcaa atgtgatgca gaacctgcat ctgattatga gcaacgggaa
      601 aagactttcc aatatggtgg gcaacctgtt tgacctttcc aatcttttca acaacaatat
      661 cgtgcttgag acttctgtat ttaatcttaa tgattttgta tataagatat cggaagccta
      721 ttctgcaata gccaaaagaa agaatgtgca gcttgtcctg aatattcctg acctgctctt
      781 cacggaggca gacaagaaca ggattttcca ggttttcaac aatcttattt caaatgctct
      841 gaaaagcata gcacagggcg aggtaagagt gactgcaaag cgttatcagg aagacaatgg
      901 aaaaattctt gttacagtga gcgatacagg tcctggactt cctaaggaaa tccgcgaact
      961 tgtttttaat gattattctg attttactcc tctggaaaaa ggaaaatcaa acggtttcgg
     1021 attcagtatg attttgacaa agaaaatcat cgaactgcac ggatgcgata tctggatgga
     1081 tgccaaatcg gacaaaggat cagttttcta ttttactatg cccgctgcca aagatgttcc
     1141 tgcatctgca gaaaaagaga aaaaaattgt atcagaagag tcgagccaga aaaagcagga
     1201 acaggataaa caggctacaa ctgtcctcat tgtcgatgat gaaccgatca acctttatgt
     1261 tctggagtct catcttgaaa aagaaggata taaagtactt caggcggtaa acgggtacga
     1321 agcacaggat atatgcaatt cagagaaaag accggatatc cttctgctgg atgtaatgat
     1381 gcccggaatt gacgggtttg aagttgtcag gaatctgaga aaaaaatact cacttcatga
     1441 acttcccata attcttctca cggctaaaaa tacaatgctg gatgttcagg aaggatttga
     1501 tgccggggca aatgattttc tgaataaacc gtttgacagg atagaacttt tgagccgagt
     1561 caatactctg atcacactga aaaaatccgt ctcggaaaat cagaagcata ttacaattca
     1621 gcatgagctt gccatcgcaa gaaaacttca gaagtctctt cttccggcaa gcaagccgga
     1681 tatggccgga attgagatgg aagccagata tattcctatg acatatctgg gaggcgacta
     1741 ctacgattat catatatctc gggaaaacca gctgggtgtg attattgccg atgtgacagg
     1801 tcatggggtt gccgctgcta tgattgcatc gatgctgaag acagcgtttc aggttcaaag
     1861 acagaatttt tccagaccgg catacgttct ggaacagatc aacaagataa tgtacgggaa
     1921 tgaacttctg actgctacat atatagtaat tgatttaaat gaaaatatat tgcgtgttgc
     1981 gaacgcggga catcctcatc tgtatgtcgc aaaaaaagac gggcagatca tttcactgaa
     2041 tccgaagggg aaactgatag gatttttccc agaaatcaaa tgcgaagaaa tctggcatcc
     2101 gatagaaacc ggagaccgaa tcattcttta cactgacgga cttgtggaag ccagcgacat
     2161 attcggagaa cccctcggag atgaaaggtt tacggaattc attcagcaga atgttcagct
     2221 ggattcaaaa gaatttatca gttccgcact tggatttgta tttgcctggt caggaaagg
//
LOCUS       DAOQGJ010000503         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31151_length_2276_cov_2.947321, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000503 DAOQGJ010000000
VERSION     DAOQGJ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31151_length_2276_cov_2.947321"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PK683_08830"
     CDS             <1..541
                     /locus_tag="PK683_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08594.1"
                     /translation="INLENNISDEAVNSLAASLGHLEKLTDLKISLRLNENRNPEKIT
                     KALTGLNQLKDLDLNLQNTALNSVPALLTHLAECSSLRNLILNLPSPDHKMIDPLILI
                     VKNLKLERAYLDCSYHSWSLDEYAKLVDEGLMKTTSLKYLYLALTEGALMFDPKVYES
                     AERLKKVIPEVFITNRDIN"
     gene            697..1110
                     /locus_tag="PK683_08835"
     CDS             697..1110
                     /locus_tag="PK683_08835"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HNH08595.1"
                     /translation="MEKQNDIRWKQRFQNYTLAFKRLEESVLQPSLNELEKNGLIQRF
                     EFTLELSWKVLKDFLEEKGFSFKPSPKDTIRLAQESSYIDYAEELIEGLEIRNILAHD
                     YSGEKFLQSEKRLREKVFPALRKLFYFLEEQAEND"
     gene            1103..1441
                     /locus_tag="PK683_08840"
     CDS             1103..1441
                     /locus_tag="PK683_08840"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNH08596.1"
                     /translation="MTESKAPSDSFGLSEKDRNIILYILKKFTEIRTVRIFGSRAKGN
                     YKKGSDIDLAVMDIIDDRILGKIQSEFEYSNLPYRVDIICYPSLTHLELKEHIDRVGI
                     VFYEKNAPPK"
     gene            1720..>2276
                     /locus_tag="PK683_08845"
     CDS             1720..>2276
                     /locus_tag="PK683_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08597.1"
                     /translation="MEISQEDIPVLSPKRWVRMLPGIWGYEIKFFNDGTFDAFFLAEP
                     TVKCTKGRFIQNLNKVSLFSEFKGIIEFRKDRSWCRKIRKDEENWYAEYDRHPDKDFR
                     HLECIYRNDPEGIIYKKHLYCSPTIRVSDPDTKFKPGDILNINGVPSISELSEGRTIT
                     GVRIREKPDLTAKILNYWGKSYLPSG"
BASE COUNT          770 a          449 c          477 g          580 t
ORIGIN      
        1 aattaacctt gaaaacaata tctctgatga agcagtgaac agtcttgcag cttctcttgg
       61 acacttggaa aaattgacag acttgaaaat cagccttaga ctgaatgaga acagaaatcc
      121 tgagaaaatt acaaaagctc tcacagggct gaatcagctc aaagacctgg atctgaatct
      181 gcagaacacc gccttgaaca gcgttcctgc cttgctgact catttggcgg aatgcagcag
      241 cctcagaaat cttatactga atctgcccag ccccgatcat aaaatgattg atcctctgat
      301 actgattgta aaaaatctga aactggagag agcgtattta gactgttctt atcacagctg
      361 gagtctggac gaatatgcaa aactggtcga tgaaggattg atgaaaacga catcattaaa
      421 gtatctgtat ttagccctga cagaaggagc actgatgttt gatccgaaag tatacgaatc
      481 tgcggaaaga ctgaagaaag tcatacctga ggttttcatc accaaccgtg atataaattg
      541 attttaagct gcagttgaag atctgcgtat gcctttgtct gaatcagact gacttttcag
      601 aagttgaagc aatgatgaat gtttccgata ctataaatat aacttgagag atttatttct
      661 tcatcagaaa aaacagaaag cagggttcat attgcaatgg aaaaacagaa tgacatccga
      721 tggaaacaga gattccaaaa ctatacactc gcatttaaac gactggagga atcagtcctg
      781 cagccaagtt tgaatgaatt ggaaaaaaac ggactgattc agcggtttga gtttacactg
      841 gaactgtcct ggaaagtact gaaggatttt ctggaggaga aaggattttc ctttaagccc
      901 agtcccaaag ataccatacg gctggcacag gaaagcagtt acatcgatta tgcggaagaa
      961 ctgatcgaag gtctggaaat cagaaatata cttgctcatg attacagcgg tgaaaaattt
     1021 cttcaatcgg aaaaaaggct aagagaaaaa gtattccctg cacttagaaa acttttctac
     1081 ttccttgaag aacaggcaga aaatgactga atcgaaggcg ccgtctgatt cattcggcct
     1141 cagcgaaaaa gacagaaata taattttata tatattaaaa aaatttactg aaatcagaac
     1201 tgtcagaatt ttcgggagca gagccaaggg aaattataaa aagggaagtg atattgacct
     1261 ggccgtaatg gatatcatag atgaccggat tttaggaaaa attcagtctg aatttgaata
     1321 ttcgaacctt ccctacagag tggatataat ctgttatccc agccttaccc acttggaact
     1381 caaagagcat attgacagag tcggaattgt attctatgaa aaaaatgcac cgccgaaata
     1441 aaatacaaag tcttataatg cgactttgtc aagtcgcact attcccatct gcagccaatt
     1501 aacacatccc ccgacggcta aagccgtcgg ggaaggggaa aacgcctgcc ggcgggctct
     1561 aagggacttc gtcccttaga aatccctaaa tactgcgtat tttatcataa atataaaagt
     1621 aattgatttt tacaacctga caaagtcgca ataattttcc tgctgctgat cctcggcaca
     1681 ttctgcactc cccgaaagga aatacggaaa aatcaggcgt tggaaatttc gcaggaagac
     1741 attccggttt taagtcccaa aagatgggtt cggatgcttc ccggaatctg gggatatgaa
     1801 attaaatttt ttaatgatgg aacgttcgat gcttttttcc ttgcagaacc cacggttaaa
     1861 tgcacaaaag gaagattcat tcagaatctg aacaaagtat ccttattttc tgaattcaaa
     1921 ggaatcattg agtttagaaa ggacagaagc tggtgcagaa agatcagaaa agatgaggaa
     1981 aactggtacg cagaatatga tcgtcatccg gacaaagatt tccgacatct ggaatgcata
     2041 taccggaatg atcctgaagg aatcatttac aaaaagcacc tctactgctc tccgacaatc
     2101 cgtgtttccg atccggatac aaaatttaaa cctggcgaca tcctgaacat aaatggcgtg
     2161 ccttccatat cagaactgtc agagggccgc acaataaccg gtgtccgaat cagggaaaag
     2221 ccggatctga cagcaaaaat tctgaactat tggggcaagt cctatcttcc ttcagg
//
LOCUS       DAOQGJ010000504         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31163_length_2275_cov_4.268018, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000504 DAOQGJ010000000
VERSION     DAOQGJ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31163_length_2275_cov_4.268018"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..633)
                     /locus_tag="PK683_08850"
     CDS             complement(46..633)
                     /locus_tag="PK683_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNH08598.1"
                     /translation="MELHKGKKTFYARTRKEWRNWLEKNHQSENSVWLIIYHKSSETE
                     SIYYNDAVEEAICFGWIDSIAHRRDDESKYQFFSRRKPKSNWSKANRERAEKMTAANL
                     MTESGKKLIELAKKTGTWDALTDVQNSVIPEDLQSELNNDKTALKNFLSFPPSSKRII
                     LEWILNAKKAETRNKRIKETVRLAAENIKANHYRQ"
     gene            complement(857..1303)
                     /locus_tag="PK683_08855"
     CDS             complement(857..1303)
                     /locus_tag="PK683_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08599.1"
                     /translation="MIYSAEKANLISEQLKKFTSGYTHHVVGHFANIDFWINEAKEAL
                     KVINDHRHRFDSTYNAQKKWIEEHQPVVHEYCPMCGGKCEFSDGKPTLPEFKHKRVLA
                     EAKKDLTDASYFFLVRCFRIGLLTKEALKEKCDLIETSIDPNDLKK"
     gene            complement(1478..>2275)
                     /locus_tag="PK683_08860"
     CDS             complement(1478..>2275)
                     /locus_tag="PK683_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08600.1"
                     /translation="WIWLFCFVLIIHPSVLSFAVSLFFCNILTGSCNYGIFNYMNINF
                     LIGAGSLIHTLLIFGTAAQVVRLGKPADFSKPDMIVRSFLILAILIFILNRTYIESFR
                     YASEYAPLILSALKTVWIFNSIVSAGFFFYLSLLLKNEFFKIPSILIIGTQMIQFLNT
                     DRGSIYLSSSGAILTSLSIAYAVWNSKVVPERLYHLRIYSIILIIKYFISYSFSAALS
                     YFGSGSLNSFYGTYSFINSIADFIFQAALFNAFWYFSEKEILSESKV"
BASE COUNT          645 a          450 c          442 g          738 t
ORIGIN      
        1 acttacattg agtatatccg aaagctggta atgcagagtt tcatcttatt gtcggtaatg
       61 atttgctttt atattttcag cggcaagtct aacagtctct tttattcttt tatttctggt
      121 ttccgctttt tttgcattca gtatccattc taaaattatc cgcttggatg acggtggaaa
      181 agaaagaaag tttttaagag cagttttgtc gttattaagt tcactctgca gatcttcagg
      241 aatgacggag ttttgaacgt cagtcagtgc atcccaggtg cctgtttttt tagccagttc
      301 aataagtttt tttccgcttt cggtcattag gtttgcggct gtcatttttt ccgctctttc
      361 ccgatttgct ttactccagt tgctctttgg ttttcgccgg gaaaaaaact ggtacttgct
      421 ttcatcatct ctcctgtgag caatactgtc tatccagccg aaacagattg cttcctctac
      481 ggcatcatta taatatatac tctcagtttc actgctttta tgataaatga tgagccagac
      541 agaattctcg gattgatgat ttttttccag ccaattccgc cattcttttc gggttctggc
      601 atagaaagtt tttttgcctt tgtgcagttc cattctataa aagggttttc ctgttaagat
      661 tcttcataaa ttgtttttac tgaacagtct gtgggttctt aaactcccgt aaaggttttt
      721 tataatagtt gtttttccgt gttacaaatt tttactgctt attgacagtg catcggcacg
      781 attcacaatc attggctcag aaacttttct tcagtctcaa cccggtgctg aatcaacggc
      841 agaggaagac aagttttcat ttcttcagat cattcgggtc tatgctggtt tcgatcagat
      901 cgcatttttc tttcagtgct tcctttgtca gaagaccgat ccggaagcac cgaacaagaa
      961 aaaaataaga ggcatccgtc agatcctttt tggcttcagc caggactctt ttatgcttga
     1021 actcgggcaa ggtcggtttt ccgtcactga attcacattt tcctccgcac atcggacagt
     1081 actcgtgtac aacgggctgg tgttcttcaa tccatttttt ctgagcattg tacgtactgt
     1141 caaaccgatg tctgtgatca ttgattacct tcagtgcctc ttttgcctca ttgatccaga
     1201 aatcaatatt cgcaaaatgt cccacaacgt gatgagtata accgctggtg aactttttca
     1261 gctgttcgga tatcagattt gctttttctg cagaataaat cattgttttg ttttccctaa
     1321 ctcatcttat gcattcatct gcagacttgc cgacagataa aaattctgtt tatgttcgcc
     1381 gcctcaaaga ttaaaaatcc tgacttgctc aatgtattca ttaaaatata tctttctgat
     1441 ttcatatctt tttttgcgaa aatgagtcag gtatttctca aaccttgctt tcagaaagaa
     1501 tttctttttc agaaaaatac cagaatgcat tgaaaagcgc tgcctggaat ataaaatcag
     1561 ctatactgtt gatgaagctg tatgtcccat agaaggaatt taagctgcct gatccaaaat
     1621 aactcaatgc tgcagaaaaa gaatagctga taaaatactt tataatcaat ataatagaat
     1681 atattcgtaa gtgataaaga cgttccggaa cgactttgga gttccatact gcataggcta
     1741 tggaaagaga cgtcagtatt gctcctgaac tgctgaggta aatactgcct cggtcagtat
     1801 tcaggaactg aatcatctgc gtacctataa tcagaatcga gggaattttg aagaattcat
     1861 tcttcagcag aagggaaagg taaaagaaaa aacctgcaga aactatggag ttgaatatcc
     1921 atactgtctt cagtgcagaa agaatgaggg gagcatattc tgaggcgtat cggaaggatt
     1981 ctatgtacgt tctgtttaaa atgaagatca gaattgccag gatcaggaaa gagcgcacaa
     2041 tcatatccgg cttggaaaaa tctgcaggct ttcccagacg gacaacctga gccgccgttc
     2101 caaaaatcag cagagtatga atcagacttc cggcgccgat aagaaagtta atattcatat
     2161 aattgaatat tccgtaatta caggagccgg taaggatatt gcagaaaaaa agagagactg
     2221 caaaactcag aacagaagga tggatgatca gaacaaaaca gaacagccag atcca
//
LOCUS       DAOQGJ010000505         2274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31189_length_2274_cov_3.525011, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000505 DAOQGJ010000000
VERSION     DAOQGJ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31189_length_2274_cov_3.525011"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK683_08865"
     CDS             complement(<1..294)
                     /locus_tag="PK683_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08601.1"
                     /translation="MKMLEYEISERIYEGVKSSIYRGTKGEQKAVIKILNGEYPSKED
                     ISRLKREADILKLFDSPYISRLYSVENHKYSPVLIEEDIGGLSLNSYNGALSLG"
     gene            complement(503..838)
                     /locus_tag="PK683_08870"
     CDS             complement(503..838)
                     /locus_tag="PK683_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH08602.1"
                     /translation="MSFNHTSENGIEKIEVEESLDLYSVPALKKYIKSLFVKGAKKVL
                     VTLQQKVFVDSSGIGMIYNLCYEADSSGIRIAFHCEEEQSAYIVQKSKMDEKLPLFTD
                     LKSALNHLE"
     gene            complement(851..1186)
                     /locus_tag="PK683_08875"
     CDS             complement(851..1186)
                     /locus_tag="PK683_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH08603.1"
                     /translation="MSYSHKVVVGVDVVELDEQIDLYTVPEVKKFCKSLLAKDNKKVI
                     ISLEKVGFIDSSGLGMLLNLKHEASTGNVGFKLAALSPEVSKTFSMTKFHDNFEIFGT
                     VNEAIRSFG"
     gene            complement(1187..>2274)
                     /locus_tag="PK683_08880"
     CDS             complement(1187..>2274)
                     /locus_tag="PK683_08880"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH08604.1"
                     /translation="VQIDFFLKQKKKFTFRKGIYELGGDINISDNIKLNNIPYIVFLN
                     GDAMGKSIQGAGGVLVLGTVFKSILQRTNDMESLRNMYPERWIKNAFIEMHKVFEGFD
                     GAMLMSLVFGVIDETTGTVYYMNAEHPDPVVYRDGKAEFLPNSGDLFRKLGTQGQSGK
                     QLQVSVFRMLPQDTLILGSDGRDDIIIGKDAVTGKDVINQDEHLFLRSVESGEGSLER
                     IYEDVMSRGDQFDDFSMLSIHFDGVSTVQGSYEKGISSIRELISAKDFKTASSELESL
                     IQIYPEYTEVFLLSAQAYMGLKNYGSAVDLAERVRLRESRNMEAYEILLDSYTALGRN
                     DRVKELVTSVNKLFPNSEKFQKFSEGK"
BASE COUNT          617 a          553 c          399 g          705 t
ORIGIN      
        1 tccaagagac aaggctccat tgtaactgtt cagagaaaga ccgccgatat cctcttctat
       61 cagaacagga gaatacttat ggttttcaac agaatagagc ctgctgatat acggcgagtc
      121 gaagagtttc aggatatcag cttcccgttt cagacggctg atgtcttctt ttgaaggata
      181 ttctccattc agaattttga tgacagcctt ctgctctccc tttgttcctc tgtaaatgct
      241 gcttttgact ccctcgtaaa tcctttcact gatttcatat tccagcatct tcatctgcat
      301 cctgcccatc tattttattt cagtacaagc cgtcaatgca tcctgtcgct gcagagtcat
      361 tcagcagcat tggaagactc ttcaggatct agaattcttc agacgcttta agtttcaaag
      421 gtcctctctg aagaattcat gaaaacagaa tggaaacaat tctatatatt tcggatgctg
      481 atccctgaaa agaattaatt tattattcga gatgattcag cgcagatttc agatccgtaa
      541 acagaggaag cttttcatcc atcttagatt tctgtactat gtaggcagac tgttcttcct
      601 cgcagtgaaa tgctattctg atccctgacg aatcagcctc atagcacaga ttgtatatca
      661 tccctattcc ggatgaatct acaaatactt tctgctgaag agtgacaagc accttctttg
      721 cacctttgac gaatagactc tttatgtatt ttttcagtgc aggcacagaa tacagatcca
      781 gtgactcttc cacttctatt ttctctattc cattttcact tgtatgattg aacgacatat
      841 ttcctccctt tcagccaaag cttctgattg cttcatttac tgttccgaaa atctcaaaat
      901 tatcatgaaa tttagtcatc gaaaaagtct tggacacttc cgggctgagt gccgccagtt
      961 tgaatccaac attgcctgtt gacgcctcat gcttcagatt cagaagcatt cccagaccgg
     1021 atgagtcgat gaacccgact ttttccaggc tgatgatcac tttcttattg tcttttgcaa
     1081 gaagcgactt acagaacttt ttgacttcag gaactgtgta cagatctatc tgttcatcca
     1141 gctcaacaac atccactccg actactactt tatgactgta agacattcat ttaccctccg
     1201 aaaatttctg aaatttttct gaatttggaa acaatttatt cacagaagtt acgagttcct
     1261 ttacacggtc atttctgccc agtgcagtgt acgaatccag aagtatttca tacgcctcca
     1321 tatttctgct ttccctgagt ctgactctct cggcaagatc tacagcggaa ccgtaattct
     1381 tcaatcccat ataagcctgt gcagacagaa gaaacacttc tgtatattca ggatatatct
     1441 ggattagaga ctctaattca gaagatgcag tcttaaaatc ttttgcactg atcagttccc
     1501 ggatactgga aatgcctttc tcgtaagaac cctgaactgt tgagactccg tcaaaatgta
     1561 ttgataacat tgaaaaatcg tcaaactgat caccgcgaga catcacatcc tcataaattc
     1621 tttcgagtga tccttcacct gattccactg acctgaggaa cagatgctca tcctggttga
     1681 ttacatcctt gcctgtgact gcatcctttc cgatgatgat gtcatccctt ccgtccgaac
     1741 ccagaatcag agtgtcctga gggagcatac ggaatacgga aacctggagc tgcttgcctg
     1801 actgtccctg tgttcccagt ttcctgaaaa gatcgcctga attggggaga aactcagcct
     1861 tcccgtcccg gtatacgaca ggatcaggat gctctgcgtt catataatac acagtacctg
     1921 tagtttcatc tattacaccg aacacaaggg acatcagcat cgcaccgtca aaaccttcaa
     1981 acaccttgtg catttcaatg aatgcattct tgatccagcg ttcaggatac atatttctca
     2041 ggctttccat atcatttgtt ctctgaagaa tcgacttgaa tactgttccg agaaccagca
     2101 ctccgcctgc tccctgaatc gacttgccca tcgcatcccc gttcaggaaa actatataag
     2161 gtatattatt cagctttata ttatcggaaa tattaatgtc tccgcccagt tcataaattc
     2221 ctttacggaa tgtgaatttc tttttctgct tcagaaagaa atcaatctga acag
//
LOCUS       DAOQGJ010000506         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31243_length_2272_cov_3.364456, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000506 DAOQGJ010000000
VERSION     DAOQGJ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31243_length_2272_cov_3.364456"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PK683_08885"
     CDS             complement(<1..687)
                     /locus_tag="PK683_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNH08605.1"
                     /translation="MNDTIGAVSTALLNSAIGIIRISGPGVLHTSSSLLHKNGKKLET
                     DYLKANARRALKCDFGNPHLDQIVFVYYADPNSYTGEDMAEFFFHGNVILMKKALSMI
                     FDSGVRPAERGEFTKRAYLSGKLDITCAEAVHRVITARSGYELELAQKNLFGETHRLV
                     SRLRSDIIGLKAECEAEIDFSTEDLTFESLEERKKRMQDVISLCDKTLRNSERAESVF
                     EKRKIVIFGEP"
     gene            complement(680..1393)
                     /gene="jag"
                     /locus_tag="PK683_08890"
     CDS             complement(680..1393)
                     /gene="jag"
                     /locus_tag="PK683_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNH08606.1"
                     /translation="MLGYIFESEGKSVAEAEASVLQLLRLKEDDLRFDVLESGKGGFL
                     GITQKKPALVRAFVASKDIPAEKIIHGVAITLLKKMGIAAEVVGMGDVDGKIYIELAS
                     QESGIIIGRRGNTLDALQFLLNLMIDSKIRHGRKIVLDIESYRDKREMSLVRLAKSMA
                     SSVAKSGKSRLLEPMNPYERRIIHMALQKDGRVFTLSDGNGTYKRVRIIPMKDKHKYK
                     DADKIKDMGSEEEDNESFE"
     gene            complement(1395..>2272)
                     /locus_tag="PK683_08895"
     CDS             complement(1395..>2272)
                     /locus_tag="PK683_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNH08607.1"
                     /translation="EEDGMRFRDKNLDPAQNKESLFAGISEKLNKSFNQGLTTPFRNG
                     IVWVLKKMYEYAIPNFGWCIIVFAILFKVAFYPLNQKQAESMKKMQELSPLLKEINEK
                     YADDPKEKQAKTLELYQKNKVNPASGCLPMIVQIPIFIALYTAFSDTIDLWKSPFLWI
                     KDLSEPDTVWQTANFYGFGAVSLNVLPLIMVGTQFFQMQMTSTAGSDPNQKTMMYMMP
                     FIMLFFFWSMPSGVTMYWTIQNILSIVQQVYTNKFVETKKNAPKKSAAATVTTTVTNT
                     SSPQYTKGSNQKKRK"
BASE COUNT          604 a          524 c          423 g          721 t
ORIGIN      
        1 cggctctccg aaaattacaa tctttctttt ttcaaatacg gactcagctc tctctgaatt
       61 cctgagggtc ttatcgcaga gagatatcac atcctgcatt cttttttttc tctcttcaag
      121 ggattcgaaa gtgagatcct ctgtggaaaa atcaatttcc gcctcacact ctgccttcag
      181 tccgataatg tcgctcctga gtctggacac cagtctgtga gtttccccga aaagattttt
      241 ctgagccagt tccagctcgt atcctgatct ggcagtaatg actcggtgaa cagcctccgc
      301 gcatgtaata tccagttttc ccgacagata ggctctttta gtaaattctc ctctttctgc
      361 cggacgcact cccgaatcaa atatcatgct taatgctttt ttcattaaaa ttacattccc
      421 atgaaagaaa aattctgcca tatcctcgcc agtgtaggaa tttggatcgg cataatatac
      481 aaatacaatc tgatccaggt ggggatttcc gaaatcgcat ttaagtgccc ttcttgcatt
      541 tgcctttaaa taatctgttt caagtttttt tccattttta tgcagaaggg atgatgaagt
      601 gtgcagaact ccgggaccgg agattctgat aataccgatg gcgctgttca gcagggctgt
      661 ggatacagcc ccgatagtat cattcaaaag actcgttgtc ctcttcttcg gagcccatat
      721 ccttgatctt gtcagcatcc ttatatttat gcttgtcttt cattggaata attctgactc
      781 ttttataggt tccgtttccg tcactcagag taaaaactct tccgtccttc tgaagagcca
      841 tatgaatgat tcttctttca tagggattca tcggttcaag cagtctggac tttccggact
      901 tagctaccga agaagccatt gattttgcaa gtctgaccag agacatttct cttttgtccc
      961 tgtacgattc aatatccagt acaatttttc ttccatgccg gatttttgaa tcaatcatca
     1021 gattcagaag aaactgaaga gcatcgagtg tattgcctct tcttcctata ataattccgg
     1081 attcctgact agcaagttct atgtaaatct ttccgtcaac atctcccatt cctaccactt
     1141 cagcggcgat tcccatcttc ttaagaagag taattgccac accgtgaatg atcttttctg
     1201 caggaatgtc tttggaagcg acaaaggccc tcacaagtgc aggcttcttc tgagtaattc
     1261 ccagaaatcc gcccttgcct gattccagaa catcaaaacg cagatcatct tccttcagcc
     1321 tgagaagctg aagaacggat gcttctgctt ccgcaactga tttaccttct gattcaaaaa
     1381 tataacctaa catattattt cctttttttc tgatttgatc cttttgtgta ctgaggagaa
     1441 gaagtattcg tcactgtagt agtcacagtc gcggcggcac tcttcttagg agcattcttt
     1501 tttgtttcta caaatttatt ggtataaacc tgctgaacaa tggagagaat attctgtatt
     1561 gtccaataca ttgtcacacc ggaaggcatc gaccagaaga aaaacagcat aatgaaaggc
     1621 atcatgtaca tcattgtctt ctgattcgga tcagatcctg cagtgcttgt catctgcatc
     1681 tgaaaaaact gagttcccac cataatcaaa ggaagaacat tcagactgac tgcaccgaaa
     1741 ccgtaaaagt ttgcggtctg ccagactgta tcaggctcgc tcagatcctt tatccagaga
     1801 aaaggacttt tccagaggtc aattgtatca ctgaatgcag tatacagtgc aatgaatata
     1861 ggaatctgaa caatcatcgg aagacatccg ctggccgggt ttactttatt cttctgataa
     1921 agctcaagag ttttggcctg tttttcctta ggatcgtcag catatttttc attgatttct
     1981 ttcagaagcg ggctgagttc ctgcattttc ttcatgcttt cagcctgttt ctggttcaaa
     2041 ggataaaatg caaccttgaa aagaatagca aaaacaatga tgcaccatcc gaagttcgga
     2101 atggcgtact catacatttt tttcagaacc catacaatcc cgttacggaa aggcgttgta
     2161 agaccttggt tgaacgattt gttcagtttt tcactgatac ctgcaaaaag agattcttta
     2221 ttctgagcag gatccagatt tttatctctg aaacgcatcc cgtcttcttc ac
//
LOCUS       DAOQGJ010000507         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31294_length_2270_cov_3.462302, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000507 DAOQGJ010000000
VERSION     DAOQGJ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31294_length_2270_cov_3.462302"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..186)
                     /locus_tag="PK683_08900"
     CDS             complement(16..186)
                     /locus_tag="PK683_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08608.1"
                     /translation="MNFLLKKQSKRNSRREGMMTLDNIRGSLLGLAAADAAGVPYEFK
                     DRNVGEWIVEAG"
     gene            360..1601
                     /locus_tag="PK683_08905"
     CDS             360..1601
                     /locus_tag="PK683_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08609.1"
                     /translation="MQSEIKILITEDDEDYRDTLIDLLSEKKFKVTAVDNGLAAEEAC
                     IGSSFDLYIVDYNMPILNGEQFVRKLKIREPDAVIIVITGEHESSRIVNIMKLGVFDY
                     LVKPVKLNYVEETVEKALEFRKFLNLQQSRQIQSAKEIQARLQWNEYKRSSINSSTDV
                     TKEILENMNRNLLQGSGIGVQITLINMLDLTKGEKDKEGNYTVAGDIFDTLIANNNFS
                     NKQLSALTNTLGIISRQNLNLKKTSSSDIIERIEIKGAQLAPFLQEKKNCRINIEHPK
                     GKFSLLTDEKFLTSALNELMINAAKYCSPNSLISIFTYSKDGYFFFSFKNSIKTEDNG
                     VPEQYEQLVLEPFFRLSKESEEAFIEFDEEFSIGLGLCVTSFIARKHNGLFWIKNYKD
                     HVSEASDLCTIAEFGLPFEKV"
     gene            complement(1617..>2270)
                     /locus_tag="PK683_08910"
     CDS             complement(1617..>2270)
                     /locus_tag="PK683_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNH08610.1"
                     /translation="RWQHPQHGLVFPDQFLARVEKMNLLPFLTEYIIDEALGFCGQLK
                     QEGYYKRISVNVTVFDLIDSSMPEYILSLIKKYSLDTEQVVIELIETGQIEENLKSLE
                     ILTRLSMKGINLSIDDFGTGYSSLNQLQKAPFNELKIDKSFVFEFVKNPDSRTIIEST
                     VDMAHKLNLEVVAEGIENRQIADMLTDIGVDTGQGYHFSMPLPAFSVTGFIRSYIYR"
BASE COUNT          726 a          457 c          430 g          657 t
ORIGIN      
        1 tacagaagac atcattcaac ccgcctccac tatccattcg ccgacattcc tgtctttgaa
       61 ttcataaggc acacctgctg catcggccgc cgcaagcccc aaaagagatc cgcgaatatt
      121 gtccaaagtc atcataccct cccgtctgct gtttcttttc gattgttttt tcagcaggaa
      181 attcattctg ctgactgaat cattatcagt caaactttgc attttatatt aaaattgcaa
      241 gcattaggtc tgaaagagaa ctccgtttcc tatgatttga acataatcga tgattcttat
      301 ctaatctgga aatttaaaat tgttgaagtg tttgtctgtg tgcaaaaact agatattgta
      361 tgcagtcaga aattaaaatt ctaatcactg aagacgatga agactatagg gatactttga
      421 tagatcttct ttcagaaaaa aaatttaagg ttactgcagt agacaatggc cttgctgccg
      481 aagaagcctg cattggatcc agttttgatc tctatatcgt agattataat atgccgatac
      541 tgaacggaga acagtttgta agaaaactga aaatcagaga gccggacgca gtaattattg
      601 ttattacagg cgaacatgag tccagcagga ttgtaaatat tatgaagctg ggcgttttcg
      661 attatctggt aaaaccggtc aagctgaatt acgttgaaga aacagtggag aaagcacttg
      721 agtttagaaa gtttctgaat cttcagcaga gcagacagat tcagtccgca aaggaaattc
      781 aggcgagact tcaatggaat gaatataaaa gatcttctat caacagctct acagacgtaa
      841 caaaggaaat tctggagaat atgaacagaa atcttcttca gggttccgga atcggcgtac
      901 agattactct gatcaatatg cttgacctga caaaaggaga aaaagacaaa gaaggaaatt
      961 atacagttgc tggagatatc tttgatacac tgatagcgaa caacaacttc agcaataagc
     1021 agctttcagc acttacaaat actctcggaa ttatttccag acagaatttg aatctgaaaa
     1081 aaacttcttc ctcagacatt attgagagaa ttgaaatcaa gggggcacag cttgctccat
     1141 ttcttcagga gaaaaagaac tgcagaataa acatagaaca tccgaaagga aaattcagtc
     1201 ttctcacaga tgaaaagttt ctgacatccg ctctgaatga actgatgatc aatgcagcca
     1261 aatactgctc tccaaattct ttgatttcaa ttttcacata ttccaaggac ggatactttt
     1321 tcttctcatt taaaaactcg ataaaaacag aagataacgg tgtgcctgag cagtatgaac
     1381 agctggtatt ggaaccgttc ttccggcttt ccaaggaatc ggaggaagca ttcattgaat
     1441 ttgatgagga gttttccatc ggactgggac tctgtgtaac aagtttcata gcaagaaaac
     1501 ataatggtct gttctggata aaaaattaca aggaccacgt cagtgaagct tcggatcttt
     1561 gcactatagc agaattcgga ctgcccttcg aaaaagtatg actgtcctgc ctgcaatcat
     1621 ctgtaaatat agcttcttat aaatcctgtt acggaaaatg caggcagagg catactgaaa
     1681 tgatagcctt gtccagtgtc cactcctata tctgtgagca tatctgcaat ctgtctgttt
     1741 tcaattccct ctgcaacgac ttcaagattc aatttgtgag ccatatcaac tgttgattca
     1801 atgattgttc tggaatccgg atttttcaca aactcaaata caaaactctt gtctatttta
     1861 agttcgttaa agggagcctt ttgaagctga ttcaacgaag agtatccagt tccaaaatca
     1921 tcaatcgaaa gattgattcc tttcattgaa agccttgtca gaatttccag actcttcaaa
     1981 ttttcctcaa tctgccctgt ttctatcagt tcaataacta cctgctcagt atccagactg
     2041 tattttttta tgagcgagag aatgtattcc ggcattgacg aatcgataag atcaaacact
     2101 gtcacattga cagatattct cttgtaatag ccttcctgct tgagctgtcc gcagaagccc
     2161 aatgcttcat ctatgatata ttcagtcaga aaaggaagaa gattcatctt ttccaccctt
     2221 gccagaaact gatccgggaa aacaagtccg tgctgaggat gctgccacct
//
LOCUS       DAOQGJ010000508         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31365_length_2267_cov_3.148282, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000508 DAOQGJ010000000
VERSION     DAOQGJ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31365_length_2267_cov_3.148282"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PK683_08915"
     CDS             <1..1398
                     /locus_tag="PK683_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08611.1"
                     /translation="RSEPEEALAHPKQVYSFPVLDYPFNTQRGSIRTENNSPVPSMEQ
                     SLHITKNAIQISHRGLLHLFEKFDRTREDPRLQRIGLIVFDYLFYALPVPTNISWMHE
                     EWHRAVLGRRNIGSQDDVYIAKPFASSISVSHVKDEDLVRLKKDHPAEMVRLSAAGME
                     SQTVLNLQLKKDSFVFGTDPSFDISSLWINSVSNTAYLQTCSGRSADHFTVKANLTEK
                     NISKRDFVGLDCNAWVYDLFRPNEPYSMRGTHWSGNGIDRYIKYSNETNLSTRIQNFL
                     MYGNDKRHSDLTKQEQDYLNLQYRLSYVNFLNPSLLGYGRFSSVNPFNGRDFYWSAGA
                     AHYLTPFGYSLNLHLFFKQDNINIVFVYHTYINAYRKLPGAEIELIRYPGTLLGKKVF
                     WNLGIHLWIQPNQQKFWTAETEAGALAKVSVSVPVSDNLEIFLGASLKSRGWVAGNVY
                     LDKAAEIRTGFSLYF"
     gene            1978..>2267
                     /locus_tag="PK683_08920"
     CDS             1978..>2267
                     /locus_tag="PK683_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH08612.1"
                     /translation="MQKDTVRIGLMAPLSGIVQLYGREICTAAQIACDEINQNGGVLG
                     KRLELIILDDGSLPETAVPAAVRLIDEFKCSALIGNLLSNSRIAVSALVSEP"
BASE COUNT          692 a          468 c          499 g          608 t
ORIGIN      
        1 cgctccgaac cggaagaggc acttgcccat ccgaagcagg tttattcctt ccctgttctg
       61 gactatccgt tcaatactca gagaggttca atccgcacag aaaacaattc tcctgttcca
      121 agcatggaac agtctcttca tattaccaaa aatgcaatcc agatttctca cagaggactt
      181 cttcatctgt ttgaaaaatt tgacaggaca agagaagatc cgagacttca gagaatcgga
      241 ctgattgtat ttgactacct gttttatgct cttccggttc cgactaacat ttcctggatg
      301 catgaagaat ggcacagggc tgttctcgga cgaaggaata tcggatcaca ggacgatgta
      361 tatatcgcaa aaccgttcgc atcctccatc agcgtaagtc atgtaaagga cgaagatttg
      421 gtccggctga aaaaggatca tcctgctgaa atggtcagac tttctgccgc aggaatggaa
      481 tcccagactg tactgaatct gcagctgaaa aaggacagct ttgtcttcgg aaccgatccg
      541 tcttttgaca tatcatcatt atggattaat tctgtcagca atactgcata tcttcagacc
      601 tgctcaggca gatcagcaga ccattttacg gtcaaggcga atctcacaga aaaaaatatt
      661 tccaaaagag actttgtcgg gctggactgc aatgcctggg tctacgatct tttccggccg
      721 aatgaacctt attcaatgag agggacacac tggagtggaa acggaatcga cagatatatc
      781 aagtacagca atgagactaa tctgagcaca agaattcaga atttccttat gtacggaaac
      841 gacaaaagac attctgacct gacaaaacag gaacaggact atctgaatct tcaatacaga
      901 ctctcctatg taaactttct gaatccgtct cttcttggtt acggcaggtt cagttcagtg
      961 aatcctttca atggaaggga tttttactgg agtgctggag cagctcatta tctgactcca
     1021 ttcggatact cattgaatct tcatctgttc ttcaagcagg acaatataaa catagtgttt
     1081 gtataccaca cttatattaa tgcctatcgg aaacttcctg gtgcagaaat tgaactgatc
     1141 agataccctg gaacccttct cggaaaaaag gtcttctgga atctcggaat ccatctctgg
     1201 atacagccga atcagcagaa attctggact gcagagacag aggccggagc actcgcaaaa
     1261 gtttctgttt ctgttccggt ttcggacaat ctggaaatct ttctgggagc atccttaaaa
     1321 agcagaggat gggtggctgg aaatgtctat ttggacaaag cagcagaaat acggacagga
     1381 ttcagcctct atttctgatc cgtatatcta ttttcacaga tcattccaga tgccttctga
     1441 agtcaccggc agaaagcttt gaagaggcgc tgatctcaga tttcaaatta cattctcacc
     1501 ttaggcaaat agaatataat tcatagcatc tataatttta gcgcagcagt agaggaatcc
     1561 agccgtttaa aactttgcga tgaatcagaa aaaacagcag tttcagattt gcggcagtat
     1621 cagatttgcc agccgtcgac aggtgttcct gctaaaaagc ggggatagca gtttcccggt
     1681 ttgcgaaatt tttttgggac tgtccggcag gactccaaac tgtcagagat ttctaatagt
     1741 cggaagggca gattgtagcc attgaaaatt cgggaaggaa gtatgaaagg atgtgtcgga
     1801 ttccctgaaa aaaactgatc gaataatgca ggaaataaaa ataaaatgat agttgagaaa
     1861 gatttatata aagaaatact ggtaagaagg tttttggtat gaattgtttc agaaatcaaa
     1921 tgagaaaatc atgaattttt aagtcaatta tcctcaatca taaacaggac agaaataatg
     1981 cagaaggata cagtcagaat cggcctaatg gcacctctct caggaatagt tcagctgtac
     2041 ggacgggaaa tttgtactgc ggcgcaaatt gcctgtgatg aaatcaatca gaacggaggt
     2101 gttttaggaa aaagactgga actgattatt ctcgacgacg gcagtctgcc ggaaactgct
     2161 gttcccgcag cagttcgact gattgacgaa tttaaatgtt ccgcgctgat aggaaatctg
     2221 ctctccaatt ccaggattgc agtttctgca ttggtttctg aacctag
//
LOCUS       DAOQGJ010000509         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31376_length_2267_cov_2.489150, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000509 DAOQGJ010000000
VERSION     DAOQGJ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31376_length_2267_cov_2.489150"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="PK683_08925"
     CDS             complement(<1..1541)
                     /locus_tag="PK683_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08613.1"
                     /translation="MLNFKVIKEKGLSFRLFWDLFMILVVLANLALIVFDLTYMYLRP
                     YYFKYKREITKSYDWVHGVEPHRLTQNYVDLVDEIVKIETDFKNNSIKSSVSSKIEKL
                     EKNMTSVRKEKNIEKFDPFLKEVEKLKTQQGRDEMLIYADISQSFFKALEETKSILEA
                     NDFDILEKDLDELNRLIRRSDEAGRTAEIESIYKKMDQLMVIIIEDDPFQKSGQTFLL
                     QEIKDRIKDNFKKNATHVEDMKYRALLSNEGYTRKSVPTSAVAFAWFWRNESQPTQQK
                     IDYFNREFRLKFELNYFPVMGKNGKQVYDFFKLDAPFFIFFLLEFIIKWILAIRRKTY
                     AGWFMYPLYHWYDVLGLIPLAEFRFFRLFRIYTMYLLLQESEYTTVGNDFITRSIRYY
                     SAIIKEELSDMVTVQILSDTQKEIKSGSSMDAFTKAVDMHRDEIKHYVISRLSDRESA
                     ERIGVLISEIVSTILGKGDFKEKFAKEAVTGILTVIAKASGNLASTPSGKAAVEKIVD
                     YVIDE"
     gene            1664..>2267
                     /locus_tag="PK683_08930"
     CDS             1664..>2267
                     /locus_tag="PK683_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH08614.1"
                     /translation="MSESETLTNRIKGIIQNKDKISVKSSRINSDLEKYVHFILSETL
                     NKFGQIRYLDMLYTILKELAINGIKANQKRIFFEENGLNIMDETQYAEGLVKFKASFS
                     DEMAEEYGEKCRKRGVFCQLDFFFTEHGMYIECVNNTPILPAEEKRLREKMKKSMAYD
                     SIAEFYMDNMDNTEGAGLGIAMTMILLKNEGIDPNLFRIVT"
BASE COUNT          654 a          464 c          437 g          712 t
ORIGIN      
        1 atttcatcaa tgacatagtc cacaattttc tcgacagcag ccttgccgga cggcgtcgat
       61 gccagatttc cggaagcttt ggcgattacc gtaagaattc cagtcaccgc ttccttggca
      121 aatttttcct tgaagtcgcc cttgccgaga attgtgctga cgatctctga aataagaact
      181 cctatccttt ctgcagattc cctgtcggaa agcctgctga ttacataatg tttgatctca
      241 tcccgatgca tatccactgc cttggtgaat gcatccatgg aagaacctga cttgatttcc
      301 ttctgtgtat cggaaagaat ctgaacggtg accatatcgg aaagctcttc cttgatgatt
      361 gcagaataat atctgatcga tcttgtaatg aaatcattcc cgactgtagt atattcgctt
      421 tcctgaagaa ggagatacat tgtatagatc ctgaagagtc ggaaaaatct gaactcggcc
      481 agaggaatca gtccgagcac atcataccag tggtaaagag gatacataaa ccatccggca
      541 tatgtttttc tccgaatggc aagaatccat tttataatga attccagaag gaaaaaaatg
      601 aaaaaaggtg catccagctt gaagaaatca taaacctgtt ttccgttttt tcccatcaca
      661 ggaaaataat tcagttcaaa tttgagtctg aattctctgt tgaaataatc tatcttctgc
      721 tgagtcggct gagattcatt tctccagaac catgcaaaag caactgctga agtggggact
      781 gactttcttg tgtacccttc attggagagc agtgccctgt atttcatatc ttccacatga
      841 gttgcattct tcttgaaatt gtcctttatc ctgtccttga tttcctgaag aagaaaggtc
      901 tggccggatt tctgaaaggg atcatcttct atgatgatca ccataagctg atccattttt
      961 ttataaatgc tttctatctc tgcagttctt cctgcttcgt cagaccttct gatcagtctg
     1021 ttcagttcat ccagatcttt ttccagaatg tcaaaatcat ttgcttccag tatgcttttt
     1081 gtctcttcca gagccttgaa aaaactctga gatatgtcgg cataaatcag catctcgtct
     1141 ctgccctgct gagtcttcag tttctccact tccttgagga acggatcgaa tttttcaata
     1201 ttcttttcct ttctgacgga agtcatattt ttctctagtt tttctatttt ggaagatact
     1261 gagcttttga tggagttgtt tttaaagtca gtttctattt tcacaatttc gtccacaagg
     1321 tctacatagt tctgagtcag tctgtgcggt tcgactccgt gaacccagtc gtaggatttt
     1381 gttatctctc ttttatactt gaaataataa ggtctcagat acatataagt cagatcaaaa
     1441 acgatcagtg ccaggttggc cagcacaaca aggatcataa acagatccca gaaaagtctg
     1501 aaacttaagc ctttttcctt gataactttg aaattaagca caatgaactc ctacgtctac
     1561 agtcccaaag cattctattt tgtcattctt agtttttctg caaaaagtaa aaattgtatt
     1621 gacctgtttt gatctgcatt tatcctgttg ctggaatttt ccaatgtcag aatcagagac
     1681 tcttacaaac agaatcaaag gcatcataca gaataaggat aagatttctg tcaagtcttc
     1741 cagaattaat tcggatctgg aaaaatatgt ccattttata ttatcggaaa cccttaataa
     1801 attcggtcag atccgttatc tggatatgct ctacactatt ctgaaagagc ttgcgatcaa
     1861 cggtatcaag gcaaatcaga aaagaatttt ttttgaagag aatggactca atattatgga
     1921 tgagactcag tacgcggagg gtcttgtcaa attcaaagcc agcttttcgg atgagatggc
     1981 tgaagagtac ggagaaaaat gcaggaagag gggtgtgttc tgtcagctcg acttcttttt
     2041 taccgagcat gggatgtata ttgaatgtgt aaacaatact cccatccttc ccgcagaaga
     2101 aaaaagactc cgcgaaaaaa tgaaaaagtc aatggcttat gacagtattg cagaatttta
     2161 tatggacaat atggacaata ccgagggcgc gggtctcgga attgctatga caatgatcct
     2221 tctgaaaaac gagggaatcg atccaaatct gttcagaatc gtaacta
//
LOCUS       DAOQGJ010000510         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31415_length_2265_cov_3.274208, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000510 DAOQGJ010000000
VERSION     DAOQGJ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31415_length_2265_cov_3.274208"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1486
                     /locus_tag="PK683_08935"
     CDS             86..1486
                     /locus_tag="PK683_08935"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNH08615.1"
                     /translation="MNFILSRNETLKSGEAAGYGFRIKNLLMLAFILADVLYCTTSIP
                     SVRPEKVPEKVTGRPWFNISYWENSDFKFRTRKLSVSSVILHNTGSRSNQEYFRETGK
                     EGYGFHFFVDAKGRTFGVPSPETAVFKLVSKMDEGALHLSYEGTKESVLSNKAQMKAL
                     SDLLRYIIMEYKVQKDNFDVAGKKGIFTHFQAKKRYGNFTDMDECGIEDVLSALLEKV
                     DGDYFAETEWKDRFNKDWIFRKEKLGKQKPMTPAQPVVRKGRGLTSQEYLKLPSLEKN
                     EKGKTPEKFRLVYNFKQKIQPVCTVLHFTAISDYQISQRVLEQRGLSATIMVDKDGKA
                     YQLLDHLDDLAQAATGTNDKCIQIEIVGKNTEELLANEFQTEKVVSLVKEISSLYDIP
                     LNNRRIEEFKGVYSHTQAKKKFGGSVALIGKDFDPGEEYMKKVLEKAGGKFYAEEDWY
                     DRKSEDWIIYYGDFQP"
     gene            1467..>2265
                     /locus_tag="PK683_08940"
     CDS             1467..>2265
                     /locus_tag="PK683_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH08616.1"
                     /translation="MAISSPEQITAIQIESVVKRFKETTAADSLNLKIHQGEFAALLG
                     PNGAGKTTLVEMIEGIQFPDSGRIQIYGMNWFSHRKELRELLGFSLQETKFLEKLTVK
                     ETICLFGSFYSKSKEESIAALHPAGLYEKRDTYVESLSGGQRQKLALCTAILHNPKIL
                     ILDEPTTGLDPGARREIWNVLLRLKTAGTTMILTTHYMEEAETLCDRILFIDRGKIIA
                     DGTLPELLKKNDHSEHILIRLNRPGKNIEWEKITGFRSIRWKEEFLEA"
BASE COUNT          715 a          430 c          557 g          563 t
ORIGIN      
        1 atttgtaaag tctgttttca tcagtatttt ctctaggaaa taatctttct tgaactttgg
       61 agtactgtta aaaatctgta aatgtatgaa ttttatactg tctcgaaacg aaactctgaa
      121 atccggcgaa gctgccgggt acggattcag aattaagaat ctgcttatgc ttgcattcat
      181 tttggctgac gttctgtact gtacaacgtc gattccttct gtcagaccgg aaaaagttcc
      241 tgaaaaagtc acaggacgac cttggttcaa tataagctac tgggagaatt cggattttaa
      301 attcaggaca agaaaactct cagtcagttc agtgatcctg cacaatacag gaagcagatc
      361 gaatcaggag tatttcaggg agactggaaa ggaagggtac ggattccatt ttttcgtgga
      421 tgcaaagggc agaaccttcg gagttccatc tcctgaaact gctgtattca agctcgtttc
      481 caagatggat gagggagctc tccatctgag ttatgaggga acgaaggagt ctgttctttc
      541 aaacaaggcg cagatgaagg ccctctcgga tcttctgaga tatattataa tggaatataa
      601 ggtgcagaag gacaattttg acgttgcagg gaagaaggga atattcactc atttccaggc
      661 aaaaaaaaga tacggcaatt ttactgatat ggatgaatgc gggattgagg atgttctttc
      721 agcacttctc gaaaaggtgg acggtgatta ttttgcggaa acggaatgga aggacagatt
      781 caacaaggac tggatattcc gaaaggaaaa actggggaag cagaaaccga tgactcctgc
      841 acagccggta gtccgaaaag gaagaggtct gacctcacag gaatatctga agcttccttc
      901 tctggaaaaa aatgaaaagg gaaagactcc tgaaaaattc cggctggtgt acaatttcaa
      961 gcagaagatt cagcctgtct gcacagttct tcattttact gcgatcagtg actatcagat
     1021 ttctcagaga gttctggagc agagggggct ttctgcaacg atcatggtgg acaaggacgg
     1081 aaaggcatat cagcttcttg atcatctgga tgatctcgca caggcggcca ctggcacaaa
     1141 cgacaaatgc attcagattg aaatagtagg aaagaataca gaagaacttc tggcaaatga
     1201 atttcagact gaaaaagtgg tttcccttgt gaaagaaatt tccagcctgt atgatatccc
     1261 tctgaacaat cgcaggatag aagaattcaa gggagtgtat tctcatactc aggcaaagaa
     1321 aaaattcggc ggctccgttg cactgatcgg aaaagatttt gacccgggtg aggaatatat
     1381 gaaaaaggtt ctggaaaagg ccggcggaaa attttatgca gaggaagact ggtatgacag
     1441 gaaaagcgaa gactggatca tctactatgg cgatttccag ccctgaacag atcacagcga
     1501 ttcagattga atctgtagtg aaaagattca aggagacgac tgcagccgac agtctgaatc
     1561 tgaaaattca tcagggagag tttgctgcgc tgctcgggcc caacggcgca ggcaagacaa
     1621 cgcttgtgga gatgatagaa ggaatacagt ttcctgattc aggcaggata cagatttacg
     1681 gaatgaactg gttttctcac agaaaggaac ttagggaact tctaggtttc tctcttcagg
     1741 aaaccaagtt tctggaaaaa ctgacagtaa aggaaacaat ctgtcttttt ggaagttttt
     1801 attccaagtc gaaggaggag agcattgctg cccttcatcc tgcaggactt tatgaaaaac
     1861 gagacaccta tgtggaatca ctttcgggag gacagaggca gaagcttgca ctctgcactg
     1921 caatacttca taatcctaaa atactgatcc tcgatgaacc gactacagga cttgatcccg
     1981 gtgccagaag agagatttgg aatgtgcttc ttcgtctgaa gactgccggc acaacaatga
     2041 tacttacaac acattatatg gaagaggcag aaactctctg cgacaggata ctgttcattg
     2101 acagggggaa aatcattgca gacgggaccc ttccggaact tctgaaaaaa aatgatcatt
     2161 ctgaacatat actgatccgg ctgaacaggc caggaaaaaa tattgaatgg gaaaaaatca
     2221 cagggttccg ttccattcgc tggaaggaag aattcctgga agcgg
//
LOCUS       DAOQGJ010000511         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31420_length_2265_cov_2.932579, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000511 DAOQGJ010000000
VERSION     DAOQGJ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31420_length_2265_cov_2.932579"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..784
                     /locus_tag="PK683_08945"
     CDS             146..784
                     /locus_tag="PK683_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08617.1"
                     /translation="MLFRLISSLAVLFATASINAEDQSVHTYKKYNLNFRLPSYWRWK
                     KENPDRKDGLIMSAKSPDKKFTLKIWHESAKISTPHKILQKFLRKKSFIMETEMKEAD
                     SEFPHLRSMDGFLVRIREKEELTGYAFSMSEGNYVFVGVLLGREKVRSPLMNEILNGF
                     EPILNREDFCCTLCLEKSEHRNLQQFCSDFIENSVCDTFFKNSRYKIRDCRL"
     gene            823..1398
                     /gene="hisB"
                     /locus_tag="PK683_08950"
     CDS             823..1398
                     /gene="hisB"
                     /locus_tag="PK683_08950"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004477621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNH08618.1"
                     /translation="MQESRKTSETDIKLDLNLYGKGIYKFDTQIPFFEHMLSHISKHG
                     NIDIDLWLRGDIEIDCHHSVEDTGILMGNMIHRLLGDKKGISRYGHFTMTMDEVLTTV
                     AVDLGGRFYFKYTGPELKGKFGIYDAELTLEFLQKFALNAKMNLHVLVHYGENRHHIH
                     ESIFKGLGRALRMAVTKDGTDSLPSTKGVLE"
     gene            1395..2030
                     /gene="hisH"
                     /locus_tag="PK683_08955"
     CDS             1395..2030
                     /gene="hisH"
                     /locus_tag="PK683_08955"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNH08619.1"
                     /translation="MIAVIDFGMGNIHSCLKAVSLYTEHFELVTDPAKVKNAAGIILP
                     GDGAFSSAMKNLEERGFKDILIERVNAGVPLFGICIGFQILFTDSDETFGRGLLVSGL
                     NLIRGHVRRFQGKNGYKVPHMGWNQLILNNRRENKILQNIPQNSYMYFIHSYRPVDVE
                     SGVVLAQCSYYNEKFPVVVEKKNIFGTQFHPEKSDKVGLKILENFIRFTDI"
     gene            2027..2233
                     /locus_tag="PK683_08960"
                     /pseudo
     CDS             2027..2233
                     /locus_tag="PK683_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
BASE COUNT          733 a          444 c          500 g          588 t
ORIGIN      
        1 ccgctggaca gaggaaaaca gaaacttcgt ctcaatatac agaatggttc gatcacggaa
       61 attcttctct gctgaaaata taagaagctg cacaaaaaca gttgacctca gtttcggctg
      121 aatcaacctt taatgtgcgg cctaaatgtt gttcagattg atatcaagtc ttgctgttct
      181 ttttgcgact gcctccataa atgcagagga tcagtctgtg catacttaca aaaagtacaa
      241 tctgaatttc aggctgcctt cctactggag atggaaaaag gaaaaccctg acagaaagga
      301 cgggctgatc atgagcgcca aaagtcctga taaaaaattc actttgaaga tctggcatga
      361 aagtgcaaaa atctccacgc ctcacaagat ccttcagaaa tttctgagaa aaaagagctt
      421 tattatggaa actgaaatga aggaagctga ttccgaattt cctcatctcc ggtcaatgga
      481 cggttttctg gtcagaatca gggaaaagga agaactgact ggatatgcat tcagtatgtc
      541 tgaagggaac tatgtatttg taggagttct tctgggaagg gaaaaggtcc gatcccctct
      601 gatgaatgaa atactgaacg gatttgaacc gattctcaac cgggaagact tctgctgcac
      661 actctgtctg gaaaaaagcg aacacagaaa tctgcagcag ttctgttccg acttcattga
      721 aaattccgta tgtgatacat tctttaaaaa ttcgagatac aaaatcaggg actgcagact
      781 ttagtcattt gacataatac agtccgtaaa aatcctgaat atgtgcagga atccaggaaa
      841 acatccgaaa ccgatatcaa actcgatctg aatctctatg ggaaaggcat ttacaaattc
      901 gatactcaga tcccgttttt cgagcatatg ctctcccata tatccaaaca tgggaatata
      961 gacatagatc tttggctcag aggggacatc gaaatagact gtcatcacag tgtggaagac
     1021 acgggaatac ttatggggaa tatgatccac cgacttctgg gcgacaaaaa aggcatcagc
     1081 agatacggcc actttacaat gacaatggat gaggttctga ctactgtggc agtcgatctg
     1141 ggcggcagat tctatttcaa atacacaggt cctgaactga aaggcaaatt cggaatctat
     1201 gacgcagagc tgactctgga gtttcttcag aaatttgcac tgaatgcaaa gatgaatctt
     1261 cacgttctgg tacattacgg cgaaaacaga catcatattc atgaatccat tttcaaagga
     1321 ctgggaaggg cactcaggat ggctgtaaca aaggacggga cagattccct cccctctacc
     1381 aaaggagttt tggaatgata gctgtaatcg attttggaat ggggaatatc cactcctgcc
     1441 tgaaagcagt ttccctttac actgaacatt ttgaactggt cactgatccg gccaaggtga
     1501 aaaatgcggc aggaatcatt cttcccggag acggagcctt ttcgagtgca atgaaaaacc
     1561 tggaagaaag aggattcaag gacatactga ttgagagagt caatgcaggt gtccctcttt
     1621 tcggaatctg catcggcttt cagatactgt ttacagattc ggacgaaaca ttcggcagag
     1681 gacttctcgt ttcaggactg aatctgatca gaggtcacgt cagacgtttt cagggaaaaa
     1741 acggatacaa ggttcctcat atgggatgga atcagctcat tctgaacaac agaagagaaa
     1801 ataagatttt acagaatatt ccccaaaatt cgtatatgta ttttattcat tcttacagac
     1861 cggtcgatgt ggaatcagga gttgtgctgg cacagtgcag ctactacaat gaaaaatttc
     1921 ctgtggttgt cgaaaaaaag aacattttcg gaacacagtt tcatccagag aagtcagaca
     1981 aggtaggatt gaaaatcttg gaaaatttta ttagatttac tgatatatga ttattatacc
     2041 tgcaattgat attttagaca atgaggctgt acgtctttac aaaggcgact actctcaaaa
     2101 aaccgtttac agcaaggctc cctgggagct gattatggca tttgaaaaga caggcgcgaa
     2161 gcttgttcat attgtagatc tgaacggtgc aagaaatgca tcggacacaa acagagatac
     2221 aatcatcaaa atcatttaga gaatctgcgt gaagcattga acaat
//
LOCUS       DAOQGJ010000512         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31451_length_2264_cov_2.336351, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000512 DAOQGJ010000000
VERSION     DAOQGJ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31451_length_2264_cov_2.336351"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PK683_08965"
     CDS             complement(<1..758)
                     /locus_tag="PK683_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH08620.1"
                     /translation="MIKKDYLIVIPVYNEAATLERVAAKVILSSAAYANVLLINDGST
                     DETPKILENLKSYFGCIGLIQKKKNEGYGATLACGFEYALMNEYKYLITMDCDDQHQP
                     EDIIRFVNESDRIDLVSGSRYMPESGSKGIRPPAERVEINFRITKLLNRRYGWNLTDS
                     FCGFKRYRLSSFAEASFEEKGYSAPMEMWSYVHSKNLTVKEIPVDKIYITSDRTFGDD
                     LDKKRKRFRYYLETWKKYHRKFSGETIWSKKAPA"
     gene            complement(918..1529)
                     /locus_tag="PK683_08970"
     CDS             complement(918..1529)
                     /locus_tag="PK683_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08621.1"
                     /translation="MNKFTRLVPFISLYLLSCLSMNAEDKTPPKIDVSSGTVIWYDGA
                     DKKTSYIMKDYVAEFEGSSGSKIKAVDKNASTLKQSVGKLKIHKVTDESIKNSLSRAE
                     VPKSLKDAGDFVPVFSDSNSEHGIGVPHNVIVGFESSVTESDAEKFAKEYKTRIKQKL
                     GFGNLYVLESDPGLAAIELANYLRTQKSVVTASPDWYRQAVRR"
     gene            complement(1542..>2264)
                     /locus_tag="PK683_08975"
     CDS             complement(1542..>2264)
                     /locus_tag="PK683_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HNH08622.1"
                     /translation="TGTAGFNTGSDKTNYSNAAYNNSFNGTSSATPLAAGVIALLLKA
                     DSALSWRDVREILAKSARKNDSTDTDWAVNGGGYNVNHKYGFGAIDANAAVTAAKTWT
                     KITGDYKSESTSSSSTVTITDNDLVTGSSATVSYSGTITKTEYVQINFTSTNSNFGHL
                     KVELTSPSGTKSVLTEVHKCISGGSIGYSLCGAASSATRFGSARHLGENPSGTWTMKV
                     YDGVSGGTPGNFSFSLTIKGRA"
BASE COUNT          578 a          513 c          433 g          740 t
ORIGIN      
        1 catgcaggtg cctttttact ccagatcgtt tctcctgaaa actttctgtg atactttttc
       61 caggtttcga gatagtacct gaatcttttt ctttttttgt ccagatcatc tccgaaagtt
      121 ctgtcggaag taatgtatat tttatctaca ggaatttcct ttacggtgag gtttttcgaa
      181 tgcacgtaac tccacatttc catcggtgca gaatatcctt tctcttcaaa tgaggcttcc
      241 gcaaatgatg acagtctgta tcttttgaat ccgcagaatg aatcagtcag attccacccg
      301 tatctcctgt tcagaagttt tgtaatcctg aaatttattt ccactctttc tgcaggaggt
      361 ctgattccct tcgaacctga ttcaggcata tatctgcttc cgcttaccag atcaatacgg
      421 tctgattcat ttacaaaccg tattatatcc tccggctgat gctgatcatc acagtccatt
      481 gtaatgagat atttatattc attcataaga gcatattcaa aaccgcaggc aagtgttgca
      541 ccgtaacctt cattcttttt tttctgaata aggcctatgc atccgaaata ggacttcaga
      601 ttctccagga ttttcggtgt ttcatccgtg gaaccgtcat tgatgagaag tacatttgca
      661 tatgcggcac tcgaaagaat tacttttgca gctacgcgtt ccagcgtggc agcttcatta
      721 taaaccggaa tgacaatcag ataatctttt ttaatcaaaa tatattcttt tcagcagaag
      781 gacttcgaac aggatctaag ttccctaatc ttttactgga accgcttact gtcttttttg
      841 aaacggaaaa aggctcctgc tccgcagttc ttcttaattt tgcggaaggg acatgttgaa
      901 agtcccttcc agttttttta tcttcttact gcctgtctgt accagtcagg ggaagcagtg
      961 actacactct tctgtgtcct cagataattt gcgagttcaa tagcagcgag gcctggatca
     1021 gattccagta cataaagatt tccgaaaccc agcttctgtt tgattctagt cttgtattcc
     1081 ttcgcaaatt tttctgcatc tgattcagtt actgaactct caaatcctac aatcacattg
     1141 tgaggaactc ctattccatg ctcagaattg ctgtccgaaa agacaggaac aaaatctccc
     1201 gcatctttga gactcttagg aacctctgcg cgggaaagag aattcttgat cgattcatca
     1261 gtgactttat gaatcttcag tttacctaca ctctgtttca gagtagaagc atttttatcc
     1321 acagccttga ttttggagcc tgaagatcct tcgaattcag ccacatagtc tttcatgatg
     1381 tatgatgtct ttttgtcggc accgtcatac cagatcacag ttccggaaga tacatctatc
     1441 ttagggggtg tcttgtcttc cgcattcatg gaaagacagg atagaaggta cagcgaaatg
     1501 aatggtacta atcttgtaaa tttgttcata attcctcctt tttatgctct tcctttaata
     1561 gtcagagaaa atgaaaagtt cccaggtgta cccccggaga ctccgtcata cactttcatt
     1621 gtccaagtac ctgaagggtt ttctccaaga tgtctggccg aaccgaatct agtggcagaa
     1681 gaagctgctc cgcagagtga ataaccgata gaccctccgg aaatgcattt atgcacttct
     1741 gtaagaacac tttttgttcc gcttggactt gtcagttcca cttttaaatg accaaagttg
     1801 gaattcgtag acgtaaagtt aatctgaaca tattctgtct tcgtaatcgt tcctgaataa
     1861 gagactgttg ctgaactgcc cgtaactaag tcgttgtcag taattgtaac tgtactggag
     1921 ctggacgttg attcactctt atagtctccg gtaatctttg tccaagtttt cgcagcagtc
     1981 actgccgcat tcgcatcaat tgccccgaaa ccgtatttat ggtttacatt gtaccctccg
     2041 ccgtttactg cccagtcggt gtcagtcgaa tcattctttc ttgcagactt tgccagaatt
     2101 tcccgaacgt ctctccagct cagtgcagaa tcagctttca gtaaaagtgc aatgactcct
     2161 gcagctaagg gagttgccga agaggttccg ttaaaactgt tattgtaagc tgcattactg
     2221 taattggtct tatcagaacc ggtgttgaat cctgctgtac ctgt
//
LOCUS       DAOQGJ010000513         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31488_length_2262_cov_3.296330, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000513 DAOQGJ010000000
VERSION     DAOQGJ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31488_length_2262_cov_3.296330"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK683_08980"
     CDS             <1..263
                     /locus_tag="PK683_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08623.1"
                     /translation="LKKLTPALGLLGLWNSMDGLGDALGRGILLKPVTELSRFFRKTL
                     IQSEIANAFVSTLIPVILEYIYKIMIVFSLVYLIRGKLIFLF"
     gene            complement(283..1242)
                     /locus_tag="PK683_08985"
     CDS             complement(283..1242)
                     /locus_tag="PK683_08985"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH08624.1"
                     /translation="MRIFVTGGSGFLGSELIARLIKEGHHVYALSRNSQSAQKFKMNG
                     VLPVEGDFNDISRWENSLNEIDAVIHCAAPVEFWGKWENFENDIVKVTKNLLLASDRQ
                     KAKRFIYISSESVLQDKEPLLDITEEHPYPEEPSSYYGKAKMLAEKEILNYGGSVHCI
                     IIRPTFIWGKGVKALDTMIDKIKSGQFMWIDSGDCPIETVHVKNVVESIILSLSSGNN
                     KSIYNVTDDSPASAREFLSALIETKGVKVPERNMPGIIASAAAVILESFWKLFGIQSV
                     PPLSRFELSFVHMPRRYNIVKIKNELGYRPVVSVKEGLREMKG"
     gene            complement(1435..>2262)
                     /locus_tag="PK683_08990"
     CDS             complement(1435..>2262)
                     /locus_tag="PK683_08990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNH08625.1"
                     /translation="IYSRAEFIVNSLAAGFAMAVLPTCSTVRTTDSKNIQAGEVKVPV
                     KGGTVPAYRAYPETGSDFPVVLVVQEIFGVHEHIRDICRRFAKLGYYAIAPELFARQG
                     DVSQMQDFKEILSKVVSKVPDEQVLTDLDASWKFAASSGLVNSEKLAITGFCWGGRIT
                     WLYAAHNPKVKAACPWYGRLSGEKNSFQTHHPLDLVPKFKNVPILGFYAGQDQGIPLD
                     QVEKMNKSLKDTGSTSEIILYPNSKHGFYADYRPMYDAKSAEDAWGKLLKHFKKNGV"
BASE COUNT          633 a          546 c          426 g          657 t
ORIGIN      
        1 aactgaagaa actcactcct gcactgggac tgctcggact ctggaattcg atggacggac
       61 tgggcgacgc actcggaaga ggaattctgc tcaagcctgt cacggaactg agcagatttt
      121 tcaggaagac cctgattcag tcggaaattg caaatgcatt tgtttccaca ctgatcccgg
      181 tcatcctgga atatatttat aaaatcatga ttgtattttc gcttgtttat ctgattcgag
      241 gaaaactgat ttttctattc tgatcagatt tcctcgggaa attcagcctt tcatctctct
      301 caggccttct ttgacgctga caacaggcct gtatcccagc tcattcttaa tcttcacgat
      361 attgtatctt ctgggcatat gaacaaaaga cagttcaaat ctggaaagag gaggaactga
      421 ctgaattccg aacagtttcc agaaggattc caggatcaca gcagcggcag atgcaatgat
      481 tccaggcata tttctctcag gaacctttac ccccttagtc tcgatcagtg cactcagaaa
      541 ttccctcgca gatgcaggac tgtcgtcagt cacattataa attgatttat tatttccgct
      601 ggataatgac agtataatgg attctacgac attttttaca tgaaccgttt caatgggaca
      661 atcaccactg tcaatccaca taaactgacc agacttgatc ttatcaatca ttgtatccaa
      721 tgccttcacg cctttccccc agataaacgt cggccggata atgatgcagt gaacagaacc
      781 gccgtaattc agaatttcct tttcagcaag cattttcgcc tttccataat aggaactggg
      841 ctcttccgga taaggatgtt cttccgtaat atccagcagc ggctctttat cctgaagaac
      901 agattccgaa ctgatataga taaaacgttt tgccttctgc ctgtctgaag caagcagaag
      961 gttctttgtg acttttacaa tatcattttc aaaattttcc cattttcccc agaactcgac
     1021 cggcgccgca cagtgaatca cagcatcaat ttcattcagt gagttttccc atctggaaat
     1081 atcattaaaa tctccttcta ccggaagtac tccgttcatt ttaaatttct gtgcagattg
     1141 agaatttctg gaaagtgcat acacatgatg accttcctta ataagccgcg ctatgagttc
     1201 cgaacctaag aacccggatc cgcctgtaac aaatattctc atataaattt ccccctgtca
     1261 aaatattgca ctgatgatgc agaagcaaag atgcttacct gcatttcata aattcgtctg
     1321 tactgacata aagccttcca aagaatcagt ttctgtcttt tctgtcattt gctcatatca
     1381 tcactcggat gccaattgat tttttctcag actcagaatc agccgacaga cggattatac
     1441 accgttcttt ttgaaatgct tcagaagttt tccccaggca tcttcagcag actttgcatc
     1501 atacataggc ctgtaatctg cataaaaacc atgttttgaa ttgggatata aaataatttc
     1561 agaagttgat cctgtatcct ttagactctt attcatcttc tccacctgat ccagaggaat
     1621 cccctgatcc tgaccggcat aaaaaccaag tataggcaca ttcttaaatt tgggaaccag
     1681 atccagagga tgatgtgtct gaaatgaatt tttttctccg ctcagtcttc cgtaccaggg
     1741 acaggctgct ttcaccttgg gattatgagc tgcataaagc caggtgatcc ttcctcccca
     1801 gcagaatcct gtaatcgcaa gcttttcaga attgacgaga ccggaggatg ccgcaaattt
     1861 ccagcttgca tccagatcag taagaacctg ttcatcaggg actttagaca ctaccttgga
     1921 aagaatctcc ttgaaatcct gcatctgaga cacgtcaccc tgccttgcaa aaagctccgg
     1981 tgcaattgca taatatccaa gcttcgcaaa ccttctgcag atatcacgga tatgttcatg
     2041 aactccgaaa atttcctgaa ctacaagaac gacggggaaa tctgaacctg tttcaggata
     2101 tgcacggtat gcaggaactg ttccgccctt cacaggaacc tttacttcgc cggcctgaat
     2161 gtttttcgag tctgttgttc tgactgtgga acaggtcggc agaactgcca tggcaaaacc
     2221 tgctgcaaga gaatttacaa taaactccgc cctggaataa at
//
LOCUS       DAOQGJ010000514         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31523_length_2260_cov_3.196825, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000514 DAOQGJ010000000
VERSION     DAOQGJ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31523_length_2260_cov_3.196825"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2179
                     /locus_tag="PK683_08995"
     CDS             <1..2179
                     /locus_tag="PK683_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNH08626.1"
                     /translation="SAYDLFINGKKIHSVGKTGGDISLETAAYLPDTVDLPHLTDKNL
                     EILIHITNYHHNKGGIWRKILFGRKKDIQRKRYLELFQDVFLAGSLFIIGIYHLGIYY
                     QIRKEKFSLYFALFCLMVFLRICFTGEMIFSYLFPNFPWNWQITFEYLNFQAAVVFGL
                     LYFATLFRKNVLQWTEKGFAGVFLALALFTLLTPPDIFTQSLFLQEMLIFAIIIYAVI
                     ILILSLKRNQDGSYVFVFGLLLLGSTVLNDILFVAGKIETGLYSPLGFFFFIFTQAFL
                     ISTFFNRALVKVANLSEKLLETNRSYGRFVPVEFLSILGKKDVTNVGLGDVIEQEVNL
                     LFCDIRSFTALSEKSKPTEVFNFLNSYFEKMNELIIRYEGIIDKYIGDAILAIFPGRP
                     ENAVNCAIEMQKLLRDYDFFLGNEKKKISAGIAIHHGQVLLGVIGGQKLIQTTVISDA
                     VNTCARLQSLTKTYGVSVIISETILNNLDDPADYRIRFLDHAVVPGKEETIYVCEIYD
                     HESEDQIRLKIQSKRAFDQGIMIYHTGDHEKAWNSFMEVLKINPQDSAAIVYMNRAAT
                     YLVQGETLIYESYDKLVKWDNSWETGIKIIDEQHKMLFDIINDLHKAMKLGREKEILS
                     RIFKSLRMYIFTHFRVEEELMHKSDYPDFTGHKNQHDQFIQYIEVMYSAFRTQQKEVT
                     SEILEFLKTWLTAHIINEDKQGYVPYIKIEKESNALNIMDVRL"
BASE COUNT          697 a          436 c          476 g          651 t
ORIGIN      
        1 atcagcctat gatctgttta ttaatggaaa gaaaattcat tctgtaggaa agacaggcgg
       61 cgatatcagc ctggaaacag cagcatatct tcctgatacc gttgatcttc ctcatctgac
      121 tgataagaat ctggagatac tgattcatat cacaaattat caccataata agggaggcat
      181 ttggagaaag atcctctttg gaagaaaaaa agacatccag cgaaaaagat atttggaact
      241 ttttcaggat gtatttcttg caggaagtct tttcataatt ggaatttatc atctaggaat
      301 ttattatcaa atcagaaaag aaaaattttc tctgtacttt gcactgttct gcctgatggt
      361 ttttctgaga atctgcttta ccggtgagat gattttctca tatctgtttc cgaactttcc
      421 ctggaactgg cagatcacat ttgaatatct gaattttcag gctgccgttg tctttggact
      481 tctttatttt gcaactctgt tcagaaaaaa tgtactgcag tggacagaga aagggtttgc
      541 cggagttttc ctggcattgg ctctgtttac cctgctcact ccccctgata tctttactca
      601 gtcccttttt ctgcaggaga tgctgatctt tgccataata atctatgccg tcatcatcct
      661 tattctgagt ctgaaaagaa atcaggacgg gtcctatgtt tttgtcttcg gacttctgct
      721 gctgggttca acagtattga acgacattct ttttgttgca ggaaaaattg aaactggcct
      781 gtactctcct ttgggatttt tcttcttcat attcactcag gcattcctga tatctacatt
      841 tttcaacagg gctctcgtga aagttgcaaa cctttctgaa aagcttctgg aaacaaaccg
      901 atcatacgga agatttgttc ctgtggaatt tctttccatc cttggaaaaa aagatgtaac
      961 gaacgtcggt cttggcgatg taatcgaaca ggaagtcaat cttctcttct gcgacatacg
     1021 gtcattcact gcactcagtg aaaaatcaaa gccgactgaa gtattcaact ttctgaattc
     1081 ctattttgag aaaatgaacg aactgatcat taggtatgaa ggaatcatag acaagtacat
     1141 cggagatgca atccttgcta tttttccagg cagaccggaa aacgcagtca actgtgccat
     1201 tgaaatgcag aagctgctca gagattatga tttttttctg ggaaatgaaa agaaaaaaat
     1261 ttctgcagga attgcaatcc accatggaca ggtgctcctc ggagtgatcg gcgggcagaa
     1321 gctcatacag actactgtaa tttccgatgc agtaaataca tgcgcaagac ttcagtcttt
     1381 gaccaagaca tacggggtat ctgtcatcat cagcgagaca attctgaaca acctggatga
     1441 tcctgctgat tacagaatcc gtttcctgga tcatgctgtt gtgccgggaa aggaagagac
     1501 gatttatgtc tgcgaaatat atgatcatga atcagaggat cagatcaggc ttaaaattca
     1561 gtccaaaaga gcatttgacc aagggataat gatttatcat acaggagatc atgagaaggc
     1621 atggaacagc ttcatggagg tactgaaaat caacccccag gattctgctg caatcgtata
     1681 tatgaacaga gcggccactt atcttgttca gggagaaact ctgatttatg aatcctatga
     1741 taagcttgtc aaatgggaca acagctggga aacagggatc aagatcattg atgagcagca
     1801 taaaatgctt tttgacatca taaatgatct gcacaaggca atgaaactgg gaagggaaaa
     1861 ggaaatactt tcccgaattt ttaaaagtct caggatgtac attttcaccc atttcagagt
     1921 ggaagaagaa ctgatgcaca aatcagacta tcctgatttt accggacaca aaaatcagca
     1981 tgatcagttc attcagtaca tagaagtcat gtattcggcg ttcagaactc agcagaaaga
     2041 agtgacatcc gaaattctgg aattcctgaa aacctggctc acagcgcata tcatcaatga
     2101 ggataagcag ggttatgtgc cttatataaa aattgaaaaa gaaagcaatg cgctgaacat
     2161 tatggatgtc cgactctgaa gatgcagtca ttcgaagaag ataaaaactg gctggaagaa
     2221 cttgtcggga ctgatcttcc gaaggaaatt gcggaaatgt
//
LOCUS       DAOQGJ010000515         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31582_length_2257_cov_2.985468, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000515 DAOQGJ010000000
VERSION     DAOQGJ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31582_length_2257_cov_2.985468"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK683_09000"
     CDS             complement(<1..355)
                     /locus_tag="PK683_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNH08627.1"
                     /translation="MKTRLKNHLQNSTELKWIPDGFHFLGPEESAKRRNLLNLLHGYF
                     NEKGFLEIQMPTFDFTSSFQNHVNPAEHGKFFKIKDGYGNEISPSHDLTLQAVKGMAG
                     FGEKKGNYRLYYISKI"
     gene            complement(373..1026)
                     /locus_tag="PK683_09005"
     CDS             complement(373..1026)
                     /locus_tag="PK683_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH08628.1"
                     /translation="MENEVTEKLWKIASRVPFPSPYFLQPSRVKKLNIPELFPEFKKY
                     FLELGSGWGEVALELAEKNPSVLYLLLEKKIDRIKKTIRNSEKKDLKNIKIITVNFNW
                     FLNEFLSENQFDEILLNFPDPWPKSRHHKHRTFDSVFAEKLSFLLKEGGRFRFASDHG
                     GYARQVIRNLRKSIHFSPPQIKFQRDDIPVSFFESEKRKEGKRIYFLETIKDCNLKT"
     gene            complement(1016..2152)
                     /locus_tag="PK683_09010"
     CDS             complement(1016..2152)
                     /locus_tag="PK683_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNH08629.1"
                     /translation="MSTEKHSFRVGLDARPLSTRVSGVGRLISEILKYFPDKINYEFI
                     LFSHLPIHESHQELLNLPNVSVQKGEGFFSKKGGLYFLFELPRQIRKMNLDVFWGSQQ
                     VAPLFIPKKLPIVLTYCDLVLYLYPDTMRKIAALQQKFFQGSSVRRADFILSISENTR
                     QDLIKKFNFPEAKTGISYPGADPARIRSFLKTPSSDKLALLPQKFMLSVSTIEPRKNY
                     PYLLEVFRKYRKLAEKPIPWVIVGKKGWETEQFYADLEHDRKTYNDIIIIDNADDADL
                     HHIYSRAELFLFASVYEGFGIPLLEALIHGRKCIASDIPTFREIGGSSIPYCSLENSD
                     EWVREILNQLKSDRVPEIDLEKFSSENSALATWEVFQKVLGLGK"
BASE COUNT          643 a          478 c          445 g          691 t
ORIGIN      
        1 agatttttga aatatagtaa agtctgtaat ttcccttctt ttcaccgaat cctgccattc
       61 cctttacagc ctgaagagtc agatcatgac tgggggaaat ctcatttccg tacccgtcct
      121 tgatcttgaa aaacttgccg tgctctgcag gattcacatg attctggaag gaagatgtaa
      181 aatcgaaggt aggcatctgt atttccagga aacctttttc gttaaaatag ccgtgcagaa
      241 gattcagcag attccttcgc ttggcactct cttccggtcc gagaaaatga aatccgtccg
      301 gtatccattt cagctcagtg ctgttctgca gatgattttt cagtcttgtc ttcaccagtt
      361 tccagagatt atttatgttt tcagattaca atccttaatt gtctccaaaa aatagattct
      421 ttttccttcc tttctttttt ctgattcaaa aaaggataca gggatatcat ctctctgaaa
      481 ttttatctga ggggggctga aatgaatgct tttccggaga ttccgaatga cctgtctggc
      541 atatcctccg tgatcggatg caaaacggaa tcttccccct tcctttaaaa gaaaggaaag
      601 tttttctgca aagacagaat caaaggtcct gtgtttatgg tgccttgatt tcggccatgg
      661 atcagggaaa ttcaggagga tttcatcaaa ctgattttca gacagaaatt cattcaaaaa
      721 ccagttgaaa tttacagtaa tgatctttat atttttcagg tctttttttt ccgaattccg
      781 gatcgtcttt ttaattcggt ctattttttt ttcgagaaga aggtacagca cagaaggatt
      841 tttttcggca agctccagtg ccacctctcc ccaccctgaa ccgagttcca ggaaatactt
      901 tttaaattca gggaacagtt caggtatgtt cagttttttc acccggctgg gctgcagaaa
      961 atacggagaa ggaaaaggaa ccctggaagc aattttccat agtttttccg tgacttcatt
     1021 ttccaagtcc aagcaccttt tgaaaaactt cccaagttgc aagagccgaa ttttccgatg
     1081 aaaatttctc cagatcaatt tcaggaaccc tgtctgattt aagctgattc agtatttctc
     1141 tgacccattc atctgaattt tcgagagaac agtacggaat ggaactgcct cctatttctc
     1201 tgaaagtagg aatatccgat gcaatgcatt ttcttccatg aatcagtgcc tctaaaagag
     1261 gaattccaaa accttcatag acagacgcga aaagaaaaag ctcagccctc gaatagatat
     1321 gatgcaggtc tgcatcatcc gcattgtcta taatgatgat gtcattatag gtcttccggt
     1381 catgctccaa gtcggcataa aactgttcag tttcccagcc ctttttgccc acaatcaccc
     1441 agggaatcgg tttttctgcc agttttctgt attttctgaa tacttccaga agataaggat
     1501 aattctttct cggctcaatc gtagaaacag aaagcataaa tttttgagga agcagagcca
     1561 gtttatccga ggaaggagtt ttcagaaaac tcctgattct tgcaggatca gctccaggat
     1621 agctgattcc ggtctttgct tcagggaaat tgaacttttt aatgaggtcc tgtctggtat
     1681 tctccgaaat ggacagaata aaatcagcac gcctgactga actgccctga aaaaatttct
     1741 gctgaagggc tgcaatcttt ctcattgtat caggataaag ataaagtaca agatcacagt
     1801 aggtcagaac aataggcagt tttttaggaa tgaaaagagg tgccacctgc tgagagcccc
     1861 agaaaacatc caagttcatt ttccgtatct gccgaggaag ctcaaagaga aaataaagcc
     1921 ctcctttttt ggaaaaaaag ccttcccctt tctgtacgga tacattcgga agattcagca
     1981 gttcctgatg agattcatga atcgggaggt gagaaaaaag aatgaattcg taattaattt
     2041 tatcaggaaa gtatttcagt atttccgaga tcagcctgcc gaccccggaa actctggtag
     2101 aaagcggtct tgcatcaaga ccgactctaa atgaatgctt ttctgtgctc aattcaaatc
     2161 tgttctgatg attgactttt cctctgcagg aacgagagca ggattcaaaa cccatgattc
     2221 tttttcggcc aaaggcttca gactgctttt atcattt
//
LOCUS       DAOQGJ010000516         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31587_length_2257_cov_2.488647, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000516 DAOQGJ010000000
VERSION     DAOQGJ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31587_length_2257_cov_2.488647"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /gene="ccsA"
                     /locus_tag="PK683_09015"
     CDS             complement(<1..359)
                     /gene="ccsA"
                     /locus_tag="PK683_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNH08630.1"
                     /translation="MKKSDFILSSHYIDIILSAVFAVLFPVSVWYSLNVPGVVLAQGV
                     SHRIFYFHVSVAWVALYAPVISSMAGILYLWKKDEKWDMISESMCRLSLIFCIAVLFS
                     GPIWAKSNWGVYWDWTDA"
     gene            complement(362..1036)
                     /locus_tag="PK683_09020"
     CDS             complement(362..1036)
                     /locus_tag="PK683_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNH08631.1"
                     /translation="MKFKMFHSLLRKELLLLFRAQNGLISMVSLILSFVYIFHFVFEK
                     DFFLERNTIAGIRWSLIYLLSFIYISQSSWEEREGGADRISSGMISGTYVYLAKTVSS
                     FSALTLSSLVLNLACIFLFEKSSMSFNEFIMQYALLFPGMLSVAAIGVCISRISEATR
                     LKEIIIPVLQIPLSLPPLLFGMNAERKMLASGKIPMEGFFAVLSFSVFYLTLGTLAEE
                     ILGGKD"
     gene            complement(1026..1631)
                     /locus_tag="PK683_09025"
     CDS             complement(1026..1631)
                     /locus_tag="PK683_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH08632.1"
                     /translation="MKKILLSCTDFTYQIGYRTLFRKAGFALFSEEILLLTGENGAGK
                     SSLLRYIYSHFREKMFQTEKNLRISYLGHETGLYSSLSLFENLQYFSGLGGHDPDMQK
                     AEFLLEGFSLKKRLHDPVFSFSEGMKKKSGLCRLFLQNAELYLLDEPFNALDKKSYDF
                     LEKIILEKKKTAGVVLVSHDERVRHIADRELRIEKENLVEV"
     gene            1630..>2257
                     /locus_tag="PK683_09030"
     CDS             1630..>2257
                     /locus_tag="PK683_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08633.1"
                     /translation="MRTLIQIFMPILCLQFLFVGNLSAEKSKVTIDKTEFETELNVKP
                     EHSGENGSFVLNSNDIAECRGREFQGEPKDSKPVTFKGKKYQSLAFLNNEGAQMLEKE
                     GGIPKAKEIFSFIVKKDPLFFPGVYNLARILQIRGEYDESLYEFGRAEKLLPDYYRTY
                     YHQGLIWKRKKKDQKALPFFRAAAKLHPFREEADYMLCETSVYSGTQDF"
BASE COUNT          734 a          497 c          468 g          558 t
ORIGIN      
        1 gcatctgtcc agtcccagta gactccccag ttgctttttg cccagatcgg tccggaaaaa
       61 agaactgcaa tgcagaatat aagagacagc ctgcacatac tttcagaaat catatcccat
      121 ttctcatctt ttttccagag atagagaatt cctgccattg aggaaattac aggagcatac
      181 agagccaccc aggcgacgct cacatgaaaa taaaatatcc tgtgggagac tccctgtgca
      241 agaactacac cgggaacatt cagactgtac catacactga caggaaaaag cactgcaaat
      301 acagcagaaa gaataatgtc aatataatgc gaagacagaa taaaatcgga ttttttcaaa
      361 atcagtcctt tcctccgagt atttcctcag caagtgtccc cagtgtcaaa taaaataccg
      421 aaaaagaaag cactgcaaaa aaaccttcca tcgggatctt tccggaagca agcattttcc
      481 tctctgcatt cattccgaaa agaagaggcg gaagagaaag agggatctgc agaacaggaa
      541 tgatgatttc cttcagcctg gtcgcttcgc tgatcctgga tatgcatact cctattgcag
      601 caacagaaag catccccgga aaaagaagtg catactgcat gataaattca ttaaaactca
      661 tggaagattt ttcaaaaaga aaaatacagg caaggttcag aacaagtgag gaaagagtca
      721 gtgcagaaaa agaagagact gtctttgcaa gataaacgta agtacctgag atcatcccgg
      781 aactgatcct gtccgctccg ccctccctct cttcccagga ggactgtgaa atatagatga
      841 atgacaggag ataaatgaga ctccatctga ttcctgcaat ggtatttctt tcgaggaaaa
      901 aatccttctc gaatacaaaa tgaaatatat atacaaaact cagtataaga gataccatgg
      961 agatcagtcc gttctgcgca cggaacagaa gcagaagctc ctttctgaga agtgaatgaa
     1021 acatcttaaa cttcaactag attctccttt tcaatcctga gctcacggtc tgcaatatgt
     1081 ctgacccttt cgtcgtgcga gacaagaact actcccgctg tcttcttttt ttcgaggatg
     1141 attttttcca ggaaatcata agatttttta tcaagtgcgt tgaacggttc gtccagaaga
     1201 tacagttccg cattctgcag aaaaagcctg cagagtcctg actttttctt catcccttcg
     1261 gagaaactga agacaggatc atggagcctt ttcttcagag aaaatccttc gagcagaaat
     1321 tcagccttct gcatatccgg atcatgtccc cccaagcctg aaaaatactg aaggttttca
     1381 aaaagtgaca gagaactgta gagtcctgtc tcatgaccaa gataggaaat cctgagattt
     1441 ttttctgtct gaaacatttt ctctctgaaa tgagaataaa tgtaccgaag caggctcgat
     1501 ttcccggcgc cgttttctcc ggtgagaagc agtatttcct ccgaaaaaag tgcaaatccg
     1561 gctttcctaa ataaggttct gtacccaatc tggtatgtaa aatcagtgca gctgaggaga
     1621 atttttttca tgagaaccct aatccagatt tttatgccca tactctgcct gcaattttta
     1681 ttcgtcggga atctgtctgc cgaaaaaagc aaagtcacaa tcgacaagac agaatttgaa
     1741 accgaactga acgtgaaacc ggaacattcc ggggagaatg gaagttttgt attgaacagc
     1801 aatgatatcg cagaatgcag gggccgcgag tttcagggag aaccgaagga cagcaagcct
     1861 gtcacattca aaggaaaaaa ataccagagc ctcgcatttc tgaacaatga aggcgctcag
     1921 atgcttgaaa aggaaggcgg gattccgaag gcaaaggaaa tcttttcctt tatcgtaaag
     1981 aaagaccctc tgttttttcc cggagtgtac aatctggcaa ggatacttca gatcagggga
     2041 gaatacgatg aatctcttta tgaattcgga agagctgaga aactgcttcc ggactattac
     2101 agaacctact accatcaggg actgatctgg aaaagaaaaa agaaagacca gaaagctctt
     2161 ccttttttca gagccgcagc aaaactgcat ccgttcagag aagaagcaga ctatatgctc
     2221 tgtgaaacgt cagtctattc aggaactcag gactttc
//
LOCUS       DAOQGJ010000517         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31658_length_2254_cov_2.255571, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000517 DAOQGJ010000000
VERSION     DAOQGJ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31658_length_2254_cov_2.255571"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..708)
                     /locus_tag="PK683_09035"
     CDS             complement(34..708)
                     /locus_tag="PK683_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH08634.1"
                     /translation="MEELKEAVNIRYAKEASCCSSLSCGGALDLADPKFGEVFIDLGC
                     GRGNDVLKAAGRVGKIGMAYGVDFTKEMIVMAEINKKKLRVENAEFLESEIEKLPFQD
                     STADVDISNCTINHSKDKAAVYKEIYRVLKTGGRFVVSDIIAENELPAEVRNDPKAWA
                     GCYGGAVPKQEYFAAMASGGFKEAEILEESEPYEKGGVIVRSITVRGHKISAPKKTCG
                     CGGGNC"
     gene            complement(920..1837)
                     /locus_tag="PK683_09040"
     CDS             complement(920..1837)
                     /locus_tag="PK683_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH08635.1"
                     /translation="MLILGGGPAGFSAAIYTSQAKLKTVIADPELTGGQVKITHLISN
                     YPGTGKAVNGYELMHNMLEQAKLSGTEIIAASDISEIQISENGQEHILSLDGGDTTVR
                     AKTLIISTGAKPRLLGVPGETEYRGQGISYCATCDGKYYDNKEIVVIGGGNSAVEESL
                     FLTRFADRVTVVQNLDRLTANQTSVEKITSHKKVKIVYNQTAYKFEKDDQGRIIVGLL
                     NLLSGEKSELKTDGVFIFIGMVPNTEGLPLSLERDKGGYLVTDEDMKTNLKGVFAAGD
                     VRSKRIRQAATAVGDGCIAGMMAAGLMAE"
     gene            1895..2206
                     /locus_tag="PK683_09045"
     CDS             1895..2206
                     /locus_tag="PK683_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08636.1"
                     /translation="MNRDQLRVVVCFAGIFGLFFQLVAETVFGASIGAGLTGTFGTLA
                     TLPVVDGAVERYTAKRDPGGPPDGPTPPGGGGSTPTPTRLRQPAQPPDDSEIRLRAVA
                     A"
BASE COUNT          496 a          622 c          476 g          660 t
ORIGIN      
        1 agcttttcat atttcttttt caaataaatc atttcagcag ttccctccgc cgcacccaca
       61 ggtttttttg ggagcagaaa tcttatgtcc acggactgta atgcttctta caatgactcc
      121 tcccttttca taaggctccg attcttccag aatttccgct tccttgaatc cgccggaagc
      181 cattgctgca aagtattcct gctttggaac cgctcccccg tagcagcccg cccaggcttt
      241 cggatcattt ctcacttcag caggaagttc attttccgca atgatgtcgg ataccacaaa
      301 tcttccgcct gtcttcaata ctctgtaaat ttctttataa acagcagctt tatctttgct
      361 gtgattgatt gtgcagttcg atatgtcgac atccgcagta ctgtcctgaa acgggagttt
      421 ttcaatttct gattccagaa attctgcatt ttccacacga agtttcttct tgttgatttc
      481 agccatcaca atcatttcct tggtgaaatc cactccgtat gccattccga ttttcccaac
      541 tcttcctgca gccttcagca catcatttcc gcgcccgcat ccaagatcaa tgaaaacttc
      601 cccgaatttc ggatcagcca gatccagtgc tcctccgcag gaaagacttg aacagcagga
      661 agcctccttt gcatatctga tatttacagc ttctttaagt tcttccatat ttttcctcca
      721 ttactctgcg ttgcagcaac ttccaagttt gcgtcgcagt tacaaatatc cctttttcta
      781 gaaattgctt ttaaaaaagc aagaaaaggt tggttcccta tcgggaggat ttaggggttt
      841 gcaaccccta agaccccgct tttaagattc ttccctacat tttctttaca acattacacc
      901 accgagcagt tcgttccatc tactcagcca tcagtccggc ggccatcatt ccggcgatgc
      961 acccgtctcc tactgcggtt gccgcctgtc tgattctctt tgatctgacg tcgcctgcag
     1021 caaaaactcc cttcaagttt gttttcatat cctcatctgt gacaagatat cctcctttgt
     1081 ctctttcgag agacaaagga agaccttccg tattcgggac cattccaatg aagataaaca
     1141 caccgtctgt tttcaattct gatttttcgc ctgacaggag attcaaaagt ccgacgatga
     1201 tcctgccctg atcatccttc tcaaatttat atgcagtttg attatatact attttaactt
     1261 ttttatgaga agtaattttt tccacagatg tctgatttgc agtcagtctg tcgaggttct
     1321 gaacgacagt gactctgtct gcaaatctgg taagaaagag agactcttcc acagcggaat
     1381 ttccgccgcc gatgaccaca atttctttat tatcataata cttaccatca caggtggcac
     1441 agtaggaaat tccctgacct cggtactcgg tctcgcctgg cacgcccagc agacggggtt
     1501 ttgctcccgt agaaatgata agcgtctttg ctctgactgt cgtatcccct ccgtcgagac
     1561 tcagaatatg ctcctgaccg ttttccgata tctgaatttc tgaaatatcg gaagctgcta
     1621 tgatttcagt cccgctcagt tttgcctgtt ccagcatatt gtgcatcagt tcatatccgt
     1681 tcacggcctt tcctgttcct ggataattcg aaatgagatg agtgattttt acctgccccc
     1741 ctgtaagttc aggatctgca atgactgtct tcagcttagc ctgagaagta taaatcgcag
     1801 cagaaaaacc tgcaggccct cctcccagaa taagcacatc tgcatatttt actgacggct
     1861 tctttttgct gatgccggcc tggaaccagg caaaatgaat cgcgaccagt tgcgggtggt
     1921 ggtgtgcttc gcaggtattt ttggtctgtt ctttcagctg gtggccgaaa ccgttttcgg
     1981 ggcatctatc ggggcgggtt tgacgggcac cttcggtacg ctggctaccc tgccggtggt
     2041 ggacggtgcg gtggagcggt acaccgcgaa gcgcgacccc ggcgggccac cggatggacc
     2101 tactccaccg ggcggcggcg gctccacccc cacccccacc cgcctgcggc agcccgcgca
     2161 gcctcccgac gattccgaaa tcaggttacg ggcggtcgca gcgtgagaag ccgggtgcgg
     2221 gacgcggagc acgcgctggt tgaggcgctg ctcg
//
LOCUS       DAOQGJ010000518         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31722_length_2251_cov_3.034153, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000518 DAOQGJ010000000
VERSION     DAOQGJ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31722_length_2251_cov_3.034153"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1133)
                     /locus_tag="PK683_09050"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="PK683_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
     gene            complement(1248..1934)
                     /gene="cmk"
                     /locus_tag="PK683_09055"
     CDS             complement(1248..1934)
                     /gene="cmk"
                     /locus_tag="PK683_09055"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNH08637.1"
                     /translation="MNENIIAVDGPAGSGKSSVAREIADRTDFHYLDSGALYRGVTLY
                     FFEKYKNLNPEQKFSEWFSSVNFLPELSSLKFEFILEKPKENILLLNGRNISAEIRTP
                     EVTEEIKYLAPLLEIRNFVNEILHGLARQYKLIMDGRDIGTEVFPDAKRKFFLTADPE
                     VRAERRWLELKEKGIVSDRKELLSDIIRRDESDKNRKIAPLRQANDAILIDTSGLEKK
                     AVIELILSKI"
BASE COUNT          587 a          526 c          422 g          716 t
ORIGIN      
        1 tcccaagtga tgtcactgat atgaatcaga ccttcgattc cattttccac ttctacgaat
       61 gcaccgtatt tagtaattcc agtgattttt ccttccagaa ctgcaccctt tctgacattg
      121 gagcttaaat tgtcccaagg attcggcagg agctgtttca ggccgagaga gagtcttttt
      181 ctctccatat cgatatccag aactacggaa tcgacttcct ggcctttttt cagaatttct
      241 ttcggatgcg ggggtttttt cgcccaggaa agttcagaat tgtgaatcag tccttccaga
      301 ccttctttca attccacgaa agcaccgaag ttagtgagag aagtgactgt tccgcgaaca
      361 accatttctt tttccagttc tcttcctgcc cagacccaag gatcttcgta catctgcttg
      421 atgcccaaag ccagacggtt gtttgcagga ttcatttcca gaatctgtac ttccacttcc
      481 tgattcggct ggaagaaatg cttgaacgga gcatactttt tgtaggaaat atcgttctgg
      541 cggagaaggc cttcgattcc catcagtttg cagaatactc cgaaacttgc gactttagtg
      601 actgtaacaa ctaccttatc gcccacagcg tgagctttga ccagttcatc ccatttctct
      661 ttgttcgttt cttctatgag ttttcttctg gagattaaag cagatttgcc tttctcgttc
      721 agtttgatga ttttaaaatc catcgtctgg gctttcagat cgtccagacc ggtatatttc
      781 atacctaact gtgaaaatgg aaggaatgtt ctcacaccct ctacatttac gataaatcct
      841 tttccctttt cttctgtctc aacacggcct gtgacctgaa gatttttggc gaaggcttct
      901 ttgagtgttt cccagccttt tctggcatcg gcttctcttt ttgaacaacg atacaatacc
      961 gaatcattgt cattttcaga accttttacg acaactgaga tgaaacttcc tctttcgggg
     1021 gcttccaaaa actcttccag cggcacaaag ccgtctgatt tagatctcag gtctacatac
     1081 aatctctggt cgattacatc cagaacctta gcttccagaa tttctccttt ccggaatctt
     1141 ttatcggtct cagcattcaa tgctgtttcc atcgggttgt ttgatttggg acggttaact
     1201 tgcgaattca atttttactc gggacacttc aacgtatttg cccaagctta tatcttggac
     1261 aggatcagct caatgacagc ttttttttcc agaccagaag tgtcaatcag gattgcatca
     1321 tttgcctgac gcaggggtgc aatttttcga tttttatccg attcgtctct ccgaattatg
     1381 tcacttaaga gttctttcct gtccgaaaca atgcccttct ccttaagttc aagccatctt
     1441 ctctctgctc tcacctcggg atctgcagtc agaaaaaact ttctttttgc atccgggaat
     1501 acttccgttc ctatatccct tccgtccata atgagcttgt attgtcttgc aagtccgtgc
     1561 aggatctcat ttacaaagtt ccgtatttcc agaagaggtg ccagatattt aatctcctcc
     1621 gtaacttcgg gagttctgat ctccgcagag atattgcgtc cattcagaag aaggatgttt
     1681 tctttcggtt tttccaggat aaattcaaat tttagtgagg aaagttcagg aagaaaatta
     1741 accgaggaga accactcaga aaatttctgt tcggggttca gatttttata tttttcaaaa
     1801 aaatataatg tcactcccct gtaaagagcg ccggaatcca gatagtgaaa atccgtcctg
     1861 tcagcaatct ctcttgccac agaacttttt ccggaccctg ccggcccgtc cactgcaatg
     1921 atgttttcat tcattcttcc tttgtcagaa gccgatttcc ggaagacaag cagaaaacgc
     1981 atacgaaaaa tacatatttg acagatcatc ggatgcaata gattttaatg aaagaacagg
     2041 tctgaggccg attccgacag aaaaaatatt tctgattgga attgaatttt cccaaaagga
     2101 aaaaaatatc attcactttg aatgggagac ggcttcccgg atgttttaaa atcaaatcat
     2161 tcagctgaaa gcaaaaattt tccgcaagat gcaaactcag acagaaaaat tcaattcttg
     2221 acagcgagta caagatactc atactatgga a
//
LOCUS       DAOQGJ010000519         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31780_length_2248_cov_3.269038, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000519 DAOQGJ010000000
VERSION     DAOQGJ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31780_length_2248_cov_3.269038"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PK683_09060"
     CDS             complement(<1..567)
                     /locus_tag="PK683_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08638.1"
                     /translation="MLLLLKGTYATDDPLKFEETNGGTGQLYQDVTTDDPVYDLTDMP
                     SAKNLLIHIDFGEVRISSRAGSPDLIQSPAESVKFWDFIAPYRQVYCTAPGYVLFANS
                     CQTDSGYFRIQEFFDGNGAKYPSVDPSAETRGGGFYGSQYYNAGIYIRNFVTSFAREN
                     NGMKTDTRFDNYPVVGSNIVPRNSYIPNT"
     gene            complement(690..1286)
                     /locus_tag="PK683_09065"
     CDS             complement(690..1286)
                     /locus_tag="PK683_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08639.1"
                     /translation="MLIFLIGTVYADESQGRKPELGFWLGASNPFPGSETSRVLNTSL
                     GFGLFGRIQWPYNLYTEAGISAANYPSLTERSLTTVPVYGALAYKLPFELPVSIFLKA
                     GGGYSYVIARPANTAKWDPMAMLGTEVSFVAGKKVRIGLRVDYLRIFETIGTDIPAEA
                     KRFYLSPLDRDYRLFNPNYYKLHDGEFFHFALMVSFLL"
     gene            1482..1826
                     /locus_tag="PK683_09070"
     CDS             1482..1826
                     /locus_tag="PK683_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08640.1"
                     /translation="MIKSSVWAILAFFVSSFLSVSSVSAKDCKGLSESQCSSNDDCTW
                     VKGYQTKDGVKVDDYCRAKPGKGDKSDKKDKKDKSDKKDKKNKKDKSDKKEKKDKKDK
                     SDKKDKKKKDKD"
     gene            1832..>2248
                     /locus_tag="PK683_09075"
     CDS             1832..>2248
                     /locus_tag="PK683_09075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNH08641.1"
                     /translation="MSKSPIFFRRNSDSSRSIELTREECSHLKSLRLFSVQKVVEIRD
                     GRGNSSFYDIPGNRETGQLQRTEKNICEPGFSAASALPKSQKLDLILQKLTELGADHI
                     HLIVFAQSDRKEFNEKRAEKIILSAAEQSERHRLPEI"
BASE COUNT          728 a          454 c          483 g          583 t
ORIGIN      
        1 ggtattcgga atataactgt tcctcggaac tatattggaa ccgactacag gataattgtc
       61 gaatctggtg tccgtcttca tcccattgtt ctctcttgca aaacttgtca caaaatttct
      121 tatatagatt cctgcattat agtactgaga tccgtaaaat cctcctcccc tggtttccgc
      181 tgatggatcc acagatggat actttgcacc gtttccgtca aaaaactcct gaatcctgaa
      241 atatcctgaa tccgtctggc aggaattggc aaataaaaca tagccgggag cagtgcagta
      301 gacctgtctg tatggagcta tgaaatccca gaattttact gattctgctg gactctgaat
      361 caggtcgggg cttcccgctc tggatgaaat tctgacttca ccgaaatcaa tgtgaatcag
      421 gagatttttt gcggaaggca tatccgtaag atcatataca ggatcatctg tcgtcacatc
      481 ctgatagagc tgtcctgttc ctccgttagt ttcttcaaac ttcaaaggat catcagtcgc
      541 atatgtacct ttcagaagca gcagcattct gttgttgaaa agactgctca ggacaggtcc
      601 tgatgtcttg gatacattct tgaaagaatt ttttttgcat ccggtcccgg aaaaaaaaga
      661 aagcagaagt aagatttgaa agaataattt catagaagaa aactcaccat caaagcaaaa
      721 tggaaaaact caccatcatg cagtttataa tagttcggat tgaaaagcct gtagtccctg
      781 tcaagaggtg acagataaaa ccttttggct tccgcaggaa tgtcagttcc aatcgtttca
      841 aaaatccgaa gataatcgac cctgagaccg attctgactt ttttccctgc aacaaagctc
      901 acctctgttc caagcattgc cattggatcc catttggcag tatttgccgg tcttgcaatc
      961 acgtaagagt atccccctcc cgccttcaga aatatggata caggaagttc aaacggaagt
     1021 ttgtatgcca gggcaccgta gacaggaacc gtcgttaaac ttctctctgt aagcgagggg
     1081 tagttcgcag cactgatgcc tgcttctgtg taaaggttgt acggccactg aattctcccg
     1141 aaaagaccga aaccgagact tgtgttcaat actcttgaag tttcagaacc gggaaacgga
     1201 ttggaagctc ccagccagaa gcccagctcc ggttttctac cctgagactc atcagcatag
     1261 acagtgccga tgagaaaaat tagcaataag aaaaagcgca agttatgttc ctaaaaacac
     1321 gaattcggca gagagcctct gtgtccatca tatatattga cttggaaaag gaggaacaaa
     1381 aaaagttttt ttcaggtata aggaataact gagaccttgg ggatttggac ttgactcata
     1441 ttccctgaaa attaggattg aacaacatta caggagtttg tatgataaaa tcatcagttt
     1501 gggctattct ggcctttttc gtttcttcat ttctctcagt cagttcagtt tcagcaaaag
     1561 actgcaaagg attaagtgaa tctcagtgca gttcaaatga tgactgtaca tgggtaaaag
     1621 gataccagac aaaagacgga gtgaaagtag acgactactg cagagcaaag cccggaaaag
     1681 gtgacaagtc tgataagaaa gataaaaagg acaagtctga caaaaaagat aaaaagaata
     1741 aaaaggacaa gtctgataaa aaagaaaaga aagataaaaa ggacaagtct gataagaaag
     1801 ataaaaagaa aaaagataaa gactgacagt cttgagtaaa tcaccgattt tcttcaggag
     1861 aaattcggac tcttcccgaa gcattgaact gacaagggaa gagtgttcac atttgaagag
     1921 tctccgtctg ttttccgttc agaaggtagt ggagatcaga gacggaagag gcaattcttc
     1981 gttttacgat attccgggaa accgagaaac aggtcagctt cagagaacag aaaaaaatat
     2041 atgtgagcct ggattttctg ctgcatcagc ccttccaaag tctcagaaac tcgatctgat
     2101 ccttcagaaa cttacagaat tgggagcaga tcatattcat ctgattgtat ttgcacagtc
     2161 ggacagaaaa gaattcaatg agaagagagc tgaaaagata attctttcgg ctgctgaaca
     2221 gtcggaaagg cacaggcttc ccgaaata
//
LOCUS       DAOQGJ010000520         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31811_length_2247_cov_3.262318, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000520 DAOQGJ010000000
VERSION     DAOQGJ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31811_length_2247_cov_3.262318"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PK683_09080"
     CDS             complement(<1..879)
                     /locus_tag="PK683_09080"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH08642.1"
                     /translation="MRLIIFGLQIILFLFITPIFSESSNSVVDLTRQKWILKKGFSEK
                     NLHPQNLSVDNDSVSAEKFPIVLNSAFKTPVSQDLQEYSMFCRFKIDLNRIEKNRELA
                     FYFSGIGESWEVFLNGVKIRDEFDIQGNEILRYRTVRNAVIPFTSSLLKEDNVLLIHT
                     AGYSPSSFLSPNNLLGLRFSKGYTLDYEKKIMDETGQISVLLLNSIYIFFGLYHLFFF
                     IRWTQKKYNLYFGVFSLLISVYFLSFSNLGYEFFSDTRPLFFFSYASQPLAVMFFMLF
                     IYDYFHPDGKFPGIIKY"
     gene            1051..1920
                     /gene="nadC"
                     /locus_tag="PK683_09085"
     CDS             1051..1920
                     /gene="nadC"
                     /locus_tag="PK683_09085"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNH08643.1"
                     /translation="MERYYTSPVSSIAPEDFDLLVELAVLEDCPDHDITSESIFPPEK
                     TGSAILITREEGIFCGSAVVESLLRKFPGSFTYEIHKSEGSIMMQGEKLLSLSGSLNV
                     ILRVERPLLNFLQYLSGIATTAWKLAREYPRLMILDTRKTLPGYRKLAKYAVFTGGGA
                     NHRISLSDMGLIKDNHVALCGSIQTAVRKIRDRFPDRKVELEIDGLHQLEDAILSEPD
                     IILLDNFSIDDTEKAVQEIRRKNNKIRIECSGGITPEKLEALSRFGDIGVSMGYLTHT
                     TRFLDLSLEIQGG"
     gene            1923..>2247
                     /locus_tag="PK683_09090"
     CDS             1923..>2247
                     /locus_tag="PK683_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH08644.1"
                     /translation="MSAFEVTPEEFLTEAEGKLSKEGFALVKRSYELSAKAHEGQKRL
                     SGQPYIIHPVNVANIIMQDLAPDDKMISAGLLHDVVEDTPYTREDMIRDFGPEIAELV
                     SGVTKV"
BASE COUNT          704 a          434 c          514 g          595 t
ORIGIN      
        1 atattttatg atgccgggga attttccgtc aggatgaaaa tagtcataaa taaaaagcat
       61 gaagaacatg actgccagcg gctgagatgc ataggaaaag aaaaacagag gccttgtatc
      121 tgaaaagaat tcatagccca gattggaaaa tgaaagaaaa tatacagaga tcagaagaga
      181 gaaaactccg aaataaagat tgtatttttt ctgagtccat ctgatgaaaa agaaaagatg
      241 ataaagcccg aaaaagatat aaattgaatt cagaagaaga acggaaattt ggcctgtctc
      301 atccataatt tttttttcgt agtccagagt gtatcctttg ctgaaccgaa gtcccagaag
      361 attgtttgga gaaagaaagg aagaaggaga gtatcctgca gtatgtatca gaaggacatt
      421 gtcctccttg agaagggaag aggtaaaagg aatgactgca tttctcacag ttctgtaccg
      481 gaggatttca tttccctgaa tgtcaaattc atccctgatc ttcactccgt tcagaaagac
      541 ttcccaggat tcgccgattc cggaaaaata aaatgcaagt tctctgtttt tttcaatcct
      601 gttcagatca attttgaatc tgcagaacat ggaatactcc tgcagatcct gtgaaacagg
      661 tgtcttgaat gccgaattca gaacaatcgg aaatttttct gcagaaactg aatcattgtc
      721 gacggaaaga ttttggggat gcagattctt ttctgaaaac ccttttttca gaatccattt
      781 ctgcctggtc agatcaacga cagaattgga agactctgaa aaaatcggtg tgatgaataa
      841 aaatagaatg atctgaagcc cgaatattat tagcctcatt tgttctccgt tttttgtgta
      901 tggaacattc tgcagaatga gcggcagaag tctaccagta aaggaaaaag ctgtgaattt
      961 cgtttttttg agacattctg aagtaactgc ttttgtcgaa taccgttccc tttacttgac
     1021 aaagtcggac tgaaataaca gactgaattt atggaaaggt actatacgag ccccgtcagt
     1081 tccattgccc cggaggactt tgatcttctt gttgaattag ccgttctgga agactgtcct
     1141 gatcatgata ttacatctga atccattttc cctccggaaa aaacgggttc tgccattctg
     1201 atcacaagag aggaaggaat attctgcggc tctgccgttg ttgaatccct tttaaggaaa
     1261 tttccgggaa gttttaccta cgaaatacat aaatcagaag gcagcattat gatgcagggg
     1321 gaaaaactcc taagtctttc cggctcactg aatgtgattc tccgtgtgga aagacccctt
     1381 ctgaattttc tgcagtatct ttccgggatt gccacgactg catggaaact tgcgagagaa
     1441 tatccccggc tgatgatcct ggatacaaga aaaactctgc cgggatacag aaaactggcc
     1501 aaatatgcag tcttcaccgg agggggtgca aatcacagga tcagtctttc ggatatgggg
     1561 ctcatcaagg acaatcatgt ggctctctgc ggttccattc agacagctgt cagaaaaatc
     1621 agggacaggt ttcctgacag gaaagtggaa ctggaaattg acggacttca tcagctcgag
     1681 gatgccattc tctccgaacc cgatatcata cttttggaca atttcagcat tgatgataca
     1741 gaaaaagcag ttcaggaaat cagaaggaaa aacaataaaa tcagaattga atgctcagga
     1801 ggaatcactc cggaaaaact ggaagcactg tccagatttg gagacatcgg agtcagtatg
     1861 ggttatctga cacatacaac aagatttctt gatctgagcc tcgaaattca agggggatag
     1921 tcatgtcagc cttcgaagta acaccggagg aatttttaac agaggcagag gggaaacttt
     1981 ccaaggaagg gtttgcgctg gtaaaaagat catatgagct ctcagcaaag gctcacgaag
     2041 gacagaaacg tctgtccggc cagccgtata tcatccatcc ggtcaatgtt gcaaatatca
     2101 ttatgcagga tcttgcaccg gacgataaaa tgatttctgc cggtctgctt cacgatgtgg
     2161 tggaggatac accgtatacc agagaggata tgattcgcga ttttgggcct gagattgcag
     2221 agcttgtctc cggagtcact aaggtat
//
LOCUS       DAOQGJ010000521         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31839_length_2246_cov_3.366956, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000521 DAOQGJ010000000
VERSION     DAOQGJ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31839_length_2246_cov_3.366956"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PK683_09095"
     CDS             <1..1101
                     /locus_tag="PK683_09095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNH08645.1"
                     /translation="RSRLNIESWKRSAVHLHKIVRNLQETALSLRMVPLSGIFRRMER
                     FILDLNRKTGKKIELKIFGERTEIDKNMVELIIDPLTHLLRNSADHGIESPEERTAAG
                     KGETGRIILSAEHAGKEVWISIEDDGRGIDREKILQKASEKGILSDSGNSLKDSEVWD
                     FIFLPGFSTAEKLTDISGRGVGMDVVKKNISKMNGSITVDSKKGEGTKILLKIPLTLA
                     IIDGMSAKYAGRYFVIPSISVQETVNLKEVQIRRIDSNFNVMKFREKMIPVIRLENFL
                     KMKSRADQAENKYVVIIESSNLRLGLILDDILGNKSIVIKPISELFSAVPGISGCTIL
                     GNGVIGLILDVNYLLDRIYYGEHSAGVRSLTV"
     gene            1103..2161
                     /locus_tag="PK683_09100"
     CDS             1103..2161
                     /locus_tag="PK683_09100"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNH08646.1"
                     /translation="MRKIRILIVDDQPIARKMIRKGLSSDPAIEVVAEAGDAYEARDR
                     IIEYSPDVMTLDVEMPKMSGLEFIRKLLPQYPIPVIMVSSSTAEGTESAVLALELGAF
                     DFIQKPDGDPEGFENMASELTEKIKSAADVNINKYIKSAGSGIPLLNRKKKPEPVLWN
                     LKYRLIAIGASTGGTAAIRSIVSRLSPDLPGIVTVIHMPAGFTKAFADRLNDLSPLQV
                     SEAYDGEEISPGKVLIAPGDLHLTVSSRNGKLYSQLSRKEKVSGHRPSVDSLFSSIAE
                     AGISEKTIGIILTGMGSDGAEGLLRLRNAGALTAGQDKETSTVYGMPKAAWDLGAVQK
                     QLRLEDIPIFLRRNAGRE"
BASE COUNT          690 a          440 c          571 g          545 t
ORIGIN      
        1 agaagcaggc tgaacattga gtcatggaaa agatctgctg ttcatcttca taaaattgtc
       61 agaaatcttc aggagactgc tctttcgctc aggatggttc ctctttcagg catattccgc
      121 agaatggaaa gattcatact ggatctgaac agaaagacag gaaagaaaat tgaactgaaa
      181 attttcggtg aacggaccga gatagacaag aatatggtgg aactgattat cgatcctctg
      241 actcatcttc tgcggaattc agcggaccac gggattgaat cccccgaaga aagaactgct
      301 gcggggaaag gtgaaacagg cagaatcatt ctttcagcag aacatgccgg caaagaagtc
      361 tggatatcca ttgaagatga cggcaggggc attgacaggg agaagattct gcagaaggct
      421 tcagaaaagg gaattctttc cgactcggga aattctctga aggattcgga ggtatgggat
      481 tttattttcc tgccgggatt ttccactgca gaaaagctga ctgatatttc aggacgcggg
      541 gtagggatgg acgtggtgaa aaagaatatt tccaaaatga acggaagcat tactgtcgat
      601 tcaaagaaag gagagggtac aaagatcctt ctcaagatcc cgttgaccct tgccatcata
      661 gacggtatgt cggccaagta tgcaggaaga tattttgtga tccccagcat cagcgtgcag
      721 gaaacagtca acctgaaaga ggttcagata cggaggatag acagtaattt taatgtaatg
      781 aaattcaggg aaaaaatgat tcctgtcatt cggctggaaa attttctgaa aatgaaaagc
      841 agggcagatc aagcagaaaa taaatatgtt gtcattatag aatccagcaa tctcagactg
      901 ggtctgattc tggatgatat tcttggaaac aaatccatcg taatcaaacc gatatctgaa
      961 cttttttccg ctgttcccgg gatctccgga tgcactattc tgggaaacgg agttatcggt
     1021 ctgatccttg atgtaaacta tctcctggac agaatttatt acggtgaaca ttccgcagga
     1081 gtccggtcct tgacggtatg atatgagaaa gatcagaata ctgatagtag acgatcagcc
     1141 gatcgcccga aagatgatca ggaagggact gtcctcggat ccagccattg aagttgttgc
     1201 agaagcagga gacgcatatg aggccagaga caggatcata gagtattcac ctgatgtaat
     1261 gaccctggat gtggaaatgc cgaaaatgag cggtcttgaa ttcataagga aactccttcc
     1321 tcagtatcct attcctgtca ttatggtaag ttcaagcacg gctgaaggaa ctgaatcggc
     1381 ggtgcttgca cttgaactgg gtgcctttga ttttattcag aagccggacg gagaccctga
     1441 aggatttgag aatatggctt cagagctcac ggagaaaatt aagtctgcgg cagatgtaaa
     1501 tataaataaa tatattaaat ctgccgggag cggtattcct cttctgaaca gaaaaaaaaa
     1561 gcctgaacct gttttatgga atttaaaata cagacttatt gcaataggag cgtctacagg
     1621 aggcactgct gcaataagat ccattgtttc caggctttct cctgatcttc ccggaatagt
     1681 cacagtgatt catatgcctg ccggttttac taaggcattt gcagacagac tgaacgatct
     1741 gtctcctctt caggtttcag aagcatatga cggagaagaa atttctccag gaaaggtgct
     1801 gattgccccc ggcgatctgc atctgactgt atcttcaagg aacggaaagc tttactcgca
     1861 gctcagcaga aaagagaaag tcagcggtca cagaccttcc gtggacagtc tcttcagctc
     1921 aatagcggaa gcaggcattt cagaaaagac cataggaatc attctgacag ggatggggtc
     1981 agacggggca gaggggcttc tcaggcttcg aaatgcaggg gcgctcactg caggacagga
     2041 caaggaaacc agtacagtat acggaatgcc taaggcggca tgggatcttg gggcagttca
     2101 aaaacagctg aggctggagg atatcccgat atttctaaga aggaatgcag gcagagaatg
     2161 aaaaattcag aaatcaaagt tctggctgcg gcagatgata atgatgcata cggtctggaa
     2221 tgcctgaaaa atctgtccca gtctca
//
LOCUS       DAOQGJ010000522         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31859_length_2245_cov_3.233790, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000522 DAOQGJ010000000
VERSION     DAOQGJ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31859_length_2245_cov_3.233790"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PK683_09105"
     CDS             <1..406
                     /locus_tag="PK683_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNH08647.1"
                     /translation="SRFFDYGPFKGEIPGRKNGVLISMDSGDTTGYALWKIQERGTLF
                     IDPVTPVYPGMIIGENARENDLEVNPVKEKKLSNVRSSGADEAIRLVPPRRLSLEQAI
                     EFIDDDELVEVTPASIRLRKKILDPSLRKRAK"
     gene            complement(709..951)
                     /locus_tag="PK683_09110"
     CDS             complement(709..951)
                     /locus_tag="PK683_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNH08648.1"
                     /translation="MYRTNECGFCNAMRKFLNKDRIHFTFYNVNSNNAKISEMREKVR
                     TVNPKAASTKFPVMNINGKIMIRPTYKQVKALVSKT"
     gene            1000..2004
                     /locus_tag="PK683_09115"
     CDS             1000..2004
                     /locus_tag="PK683_09115"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH08649.1"
                     /translation="MMKSGHFKGNSGFEEFIPPRMLRHNMVQSWLASYKRKDWQNFPF
                     VKKSEWVLLEVPDNVRLLASVSVHPNSKGLFILLHGWEGGIDSGYILRTGNYFWNSGY
                     SIARLNLRDHGSTHHLNEEAFNGSMLDETFEAVRKISDMFPDQDCRVIGFSLGGNFAC
                     RIAVRNSSERNSIRTLKQCFAVSPPLDPYKATYTMDRHKILRKYFLRAWKDSLEKKKR
                     LFPHLYADEKIDSSRTVMDLTEQLIPKYTEYKNIYSYFSVYTLKNSYFAGLSTPLFIL
                     SSKDDPVIPPEEFIALIPGKFLKVHLSEYGGHCGFIKNLKLDSWVWDWVEQSQRDAEL
                     "
BASE COUNT          652 a          446 c          510 g          637 t
ORIGIN      
        1 aagcagattc tttgattatg gtcctttcaa gggagagatt cccggaagaa agaacggtgt
       61 gctgatttct atggactctg gagatacaac aggttatgct ctctggaaaa tccaggaaag
      121 aggaactctt ttcattgacc cggtcactcc tgtctatccg ggaatgatca tcggtgaaaa
      181 tgcaagagaa aacgatctcg aagtcaatcc tgtcaaggaa aagaaactga gcaatgtgag
      241 atcttccgga gcagatgagg caatccgtct cgttcctcct aggagactct ctcttgagca
      301 ggcaattgaa tttattgatg atgatgagct tgtagaggtt acacctgcat ccatacgtct
      361 tcgcaagaaa attctggatc cttccctcag aaaaagggct aagtaaggca gtgaaaagtg
      421 aatgcggctt cgtctagtcg cattcacagc ggctttgatt ttttaacttt ttgggactgt
      481 gtaaaaagat cgttttcacg cagggaccca aagacgcaga gaaaaaagaa ccgcaagttt
      541 ggaagcttct gataaaactt gcactgagta cagtcaagcc tctgcgaaca ctgcgacaag
      601 cctaggtgca ctgccccgtg actccgcgtg agtcttgttt ttcttcgctc ttgcacggat
      661 ccgaggaaag aaaatcataa agtcagtgaa attagagaaa aaggaatatc aggttttaga
      721 aactaaagcc ttgacttgtt tgtaagtggg gcggatcata atctttccgt ttatgttcat
      781 tactggaaat tttgttgaag cagctttagg attgacggtg cggacttttt cccgcatttc
      841 cgaaattttt gcattgtttg aattcacatt ataaaaagtg aaatgaattc tgtctttgtt
      901 cagaaacttt ctcattgcat tgcagaaccc gcattcgttt gtcctgtaaa tgaatatttt
      961 tgcttttcga aaaaatcctt tacttcagac tctgtacaaa tgatgaaaag cggtcatttc
     1021 aagggaaaca gtggttttga ggagtttatt cctccgcgta tgctccggca caatatggtg
     1081 cagtcctggc ttgcctctta taaaaggaaa gactggcaga atttcccttt tgtaaaaaaa
     1141 tcagaatggg ttcttcttga ggttcctgac aatgtccgtc ttcttgcttc agtatccgtt
     1201 catccgaatt caaaaggact tttcattctg ctccatggtt gggaaggagg aatcgattcc
     1261 gggtatattc tcaggacggg aaattatttt tggaacagtg ggtattcgat tgcaaggctg
     1321 aatcttcgcg atcatggaag cactcatcat ctgaatgaag aagcattcaa cggaagtatg
     1381 cttgatgaaa catttgaggc agtcagaaaa atatcggata tgtttccgga tcaggactgc
     1441 agggtgatcg gtttttcact cggcggaaat ttcgcctgca ggatcgccgt cagaaattct
     1501 tcagaaagga acagcatccg caccctgaag cagtgttttg ccgtgagtcc tcctctcgat
     1561 ccatataagg ctacctatac gatggacagg cataaaattc tcaggaaata tttcctgcgt
     1621 gcctggaagg attcccttga aaaaaagaag cgcctcttcc cgcacctgta tgccgatgaa
     1681 aaaattgact caagcagaac tgtaatggat ctgacagaac agctgattcc taaatatacc
     1741 gaatacaaga atatttacag ttatttttcc gtttatacgc tgaaaaactc ctattttgca
     1801 ggactgtcca ctcctctgtt cattttgagc tccaaggacg acccggtgat tcctccggaa
     1861 gagttcattg ctctgattcc tggaaaattt ctgaaagttc atttgagcga atacggaggg
     1921 cactgcggat ttataaaaaa tctgaagctt gattcctggg tctgggactg ggtggaacaa
     1981 tctcagcggg atgctgaatt ataattttta ctgagcatag agtcataaag actgatgcag
     2041 ttctcagccg actgaaatag cctgagccgg atctgaaaaa aacaggattg gatcgaattc
     2101 acttgaaaag gaacggttcg gacaaggata tggttttcga aaatcagtaa ctcaccctac
     2161 gtaaatatga tattctgcct ggaggcagca attttcagaa aatatcagga aaatcaggca
     2221 ttttacaagt atgcaatgaa tcagt
//
LOCUS       DAOQGJ010000523         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31908_length_2243_cov_3.292962, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000523 DAOQGJ010000000
VERSION     DAOQGJ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31908_length_2243_cov_3.292962"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PK683_09120"
     CDS             <1..689
                     /locus_tag="PK683_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08650.1"
                     /translation="SETEKNRTYFFSVEDTGIGIPAEKIPILFDKFTQVNVSTTREYG
                     GTGLGLAICKKIAELMNTSISVESESGKGSKFFFTAEFPKAENIPELIEAIQPTCELK
                     GVRALVVDDNEINTVVAESFLEKWGCRVDIADSGEKALQLLSKEDYNVILMDIQMPEM
                     DGWQTSEAIRRMEDSKKSSVPIIALTAGSHTECEKEKSRLNGFVRKPFSPEELLNALK
                     SVLNWPNSNH"
     gene            818..>2243
                     /locus_tag="PK683_09125"
     CDS             818..>2243
                     /locus_tag="PK683_09125"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNH08651.1"
                     /translation="MTEKIITKSRFLQGLRCRNLLWNIYSDPSAIEEDPQAKMKLNPQ
                     YRQMMTLFRSAFPGGTEIREGLSYEEGRRITQDRLNTEKILFNAVFLENDCFSRIDVL
                     EKSGDAWNIYEVRSTVRNRTDSYIDTAFQKKTVEASGIKTGNCFIVLINPAYILGETF
                     EIENFFKIKNVTVETDKAVPHMESLLKKVKITAALSEFPPRNEESCRSPKDCRSREKC
                     WKGVELGDIFSLREGREISIQLFNAGIKYMKDIPPETDLNTAQKIQISSYLENRPFIN
                     KEKIQDFINKVKYPASFLDFETVNPSIPIFAKTAPYQHVPFLFSLHILEEDKSITHKT
                     WISRNHGDPREEILKSLKEWIPEGSGSIICFNDMIERKCLKDSAAVYTEYQNWVQTVL
                     LRFQDISNVFRNFQYYHPMQNGSASLKSIYPALTGEPGYGELTIKDGGSANLEYLQFL
                     LQEKEADPKLLSDLEKYCSMDTYAM"
BASE COUNT          755 a          430 c          487 g          571 t
ORIGIN      
        1 tttcagagac tgaaaaaaac cgtacttact ttttttcagt ggaagatact ggcattggaa
       61 ttcctgccga gaagatccct attctcttcg ataagttcac tcaggtaaac gtatcaacga
      121 ccagagaata cggaggaacc ggtctgggac ttgccatctg caaaaaaatc gctgaactga
      181 tgaatacgtc catatcagtg gaatcagaat cagggaaagg ttcaaagttt tttttcaccg
      241 cagagtttcc taaagcagag aacattccgg aactgattga agccattcag cctacctgtg
      301 aactgaaagg agtcagggca cttgtggtgg atgacaatga aatcaataca gtcgtagcag
      361 aaagttttct ggaaaaatgg gggtgcagag tagacattgc agacagcggt gaaaaagcac
      421 tccagcttct ttctaaagag gattataatg taattttaat ggatattcag atgccggaaa
      481 tggacggctg gcagacatct gaagccatcc gcagaatgga ggattccaaa aaatcatctg
      541 ttccaatcat tgcgctgaca gctggatctc atacagaatg cgaaaaagaa aaaagcaggc
      601 tgaacggatt tgtcagaaaa cccttcagtc cggaagaact tttaaatgct cttaagtctg
      661 ttctgaattg gccgaattcc aatcactgac atctgatcaa aaatcagaaa gaaagtaaaa
      721 agccatattt ttaatacttg gctttttgga ctgtccggaa ataattaact gaccttagag
      781 aaagtttttg aattctttag aataagacag gtttgaattg acagagaaaa tcattacaaa
      841 atccagattc ctgcagggac tcagatgcag gaatcttcta tggaacatat acagcgatcc
      901 ttccgcaata gaggaagatc ctcaggcaaa aatgaaactc aatcctcagt acagacagat
      961 gatgaccctg ttcagaagtg cctttcccgg aggaacggaa atcagggagg gactgagcta
     1021 cgaggaaggc agacggatca ctcaggacag acttaataca gaaaaaattc tgttcaacgc
     1081 ggtctttctg gaaaatgact gtttcagcag aattgatgtg ctggagaaat ccggtgatgc
     1141 atggaatatc tatgaagtaa gatctacagt cagaaacaga acggacagtt acattgatac
     1201 ggcattccag aaaaaaaccg tagaagcttc gggcataaaa acaggaaact gctttattgt
     1261 tctcatcaat ccagcctata ttctgggtga aacatttgaa attgaaaact tttttaaaat
     1321 aaaaaatgtt acagttgaga cggacaaagc agtcccgcat atggagtctc tgctgaaaaa
     1381 ggtgaagatt actgcagcac tctccgaatt tccgcctaga aatgaagaat cctgcagatc
     1441 tcctaaggac tgcagatcca gagagaaatg ctggaaaggg gttgaactgg gagatatttt
     1501 cagcctgaga gagggacggg aaatttcaat tcagcttttc aatgcgggaa tcaagtacat
     1561 gaaagatatt cctcctgaaa cagatctgaa cactgcacag aagattcaaa tctcatccta
     1621 tctcgaaaac aggcctttca taaataagga aaaaatacag gattttatca ataaagtaaa
     1681 gtatccggca agttttctgg acttcgaaac ggtaaatccg tccattccta ttttcgccaa
     1741 gactgctcca tatcagcatg taccttttct tttttccctt catattcttg aagaagacaa
     1801 aagtatcact cataaaactt ggatcagcag gaatcacggt gatcctagag aggaaatttt
     1861 gaaaagtctg aaagaatgga taccggaagg atcagggagc atcatctgtt tcaatgatat
     1921 gatagaaaga aaatgtctga aagactctgc tgcagtttat accgaatacc agaactgggt
     1981 ccagactgtt cttctgcgtt ttcaggatat ctccaatgtg ttcagaaact ttcaatacta
     2041 ccatcctatg cagaacggaa gcgcatctct gaaaagtatt tatcctgcac ttacgggaga
     2101 acctggatac ggtgaactga caattaaaga tggaggatcg gcaaacttgg aatatcttca
     2161 gtttctgctt caggaaaaag aagctgatcc caaactgctg tctgatctgg aaaaatactg
     2221 ctcgatggat acttatgcga tgg
//
LOCUS       DAOQGJ010000524         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31924_length_2242_cov_3.421125, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000524 DAOQGJ010000000
VERSION     DAOQGJ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31924_length_2242_cov_3.421125"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK683_09130"
     CDS             complement(<1..218)
                     /locus_tag="PK683_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08652.1"
                     /translation="MSEISNEDIMKIIDNASEKSMDEEPEINLTEDARITKSAYKDDM
                     VKKIIEGSTEEFVAKQQKEQADILSDEI"
     gene            complement(229..>2242)
                     /locus_tag="PK683_09135"
     CDS             complement(229..>2242)
                     /locus_tag="PK683_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH08653.1"
                     /translation="NAILDLSEDVIDQINQRVFQNAKNYLMTAVDMTELSSKITVEGV
                     ETLDNNPRLDGLMIAILKLHPQLKNFEIGNDNGDFLMQRREQDGTISTKVVTRKKGKV
                     TVKWRRRDLEGKIIKEDLDTEDKYDPRERPWYQGAKREGKLFWSDVYIFATDKVPGVT
                     ASSPVRDKDGKFKGVFGLDIPLVAISEFLMNLKDELKNQNIGKKSIIYITNSKGEIVA
                     FPDSSVPMILEEGKLRPRKVEESDFPEIKETYKYFYETKDSKFNLEVSGVKYLASYKE
                     FPKKDFDKDWTIGLVVPEDDMIGPIKRTNSLLITVSGVILAGAMILGLIMFRLKKALD
                     VRNRFIKETFGMYLSDEVVNSILETPEGTMLGGDKRIVTIMMTDLRGFTSIGERLPAE
                     SVVGMINIYLDIMTDIIFKYNGTIDEFIGDAILVIFGAPIQRDDDAQRAVACALEMQL
                     AMDEVNRRNREAGFPEVQMGIGINTGDIVVGNIGSKKRMKYGVVGKNVNLTSRIESYT
                     VGGQTLISPFTVDACKGLLRIDDQFEVMPKGVNTPITISAVGGIGGPFNVYMPEKKDA
                     VLKKVKSKVHFEFNILAGKHASDDLHLGDLLELGEKEGILGTHIPVEKLNNIKILRVF
                     INGKEYKTDLYAKVTQNISNAQLRINFTSVPPDSGSVLKDILGNHT"
BASE COUNT          556 a          535 c          398 g          753 t
ORIGIN      
        1 ttgatttcat cagaaagaat gtcagcctgt tctttctgct gctttgcaac aaattcttca
       61 gtgcttcctt ctatgatttt ttttaccata tcgtccttgt aggcggattt ggtaattctt
      121 gcatcctctg taagattaat ttcaggctct tcatccatag atttctctga ggcattatcg
      181 atgattttca tgatatcttc attagatatt tcggacattt tattctcctt aagtatgatt
      241 gccaagtatg tctttgagaa ctgaacctga atcgggagga actgaagtaa aattgatcct
      301 caactgtgca tttgaaatgt tctgagtcac ttttgcatac aggtctgtct tgtattcttt
      361 cccgttgata aaaactctta gaatcttgat attgttcagt ttctccacag ggatatgagt
      421 cccgagtatc ccttcttttt cacccagctc aagcagatct cccagatgaa gatcatcact
      481 cgcatgtttt cctgccagaa tgttaaactc aaaatgcact ttagatttta cttttttcag
      541 aactgcatct tttttctcag gcatatatac attgaaaggt cctccgattc cgcccacagc
      601 actgattgta atcggagtgt tcactccctt cggcatcact tcaaactgat cgtcaattct
      661 cagaagtccc ttgcaggcat ctacagtaaa aggggaaatc agagtctgtc ccccgacagt
      721 ataggattca attctggaag taaggtttac attctttcct acaaccccgt atttcatcct
      781 tttctttgat ccaatgtttc cgactacgat atcgcctgta ttaattccaa tccccatctg
      841 aacttcagga aatcctgctt ccctgtttct tctgtttact tcatccattg ccagctgcat
      901 ttcgagagca caggcaacgg ccctctgtgc atcatcgtct ctctggatcg gtgcaccgaa
      961 aattacaagg atagcatccc cgataaattc atcaatagtt ccattatatt tgaatataat
     1021 atctgtcata atatccaaat atatattgat cattccgact acactttcgg cgggaagtct
     1081 ttcccctatg gacgtaaatc ctctgagatc tgtcatcatg atggtcacaa ttctcttgtc
     1141 tccgccgagc atggttcctt caggagtttc cagaatgctg ttgacaacct cgtcagaaag
     1201 atacattccg aatgtttcct tgatgaatct gtttcttaca tcaagcgctt tcttcagacg
     1261 gaacataatc agtccaagaa tcattgcacc ggccagaatc accccggaaa cagtgatcag
     1321 aagactgttc gttctcttga taggaccgat catatcgtct tccggaacga ctagtccgat
     1381 tgtccagtcc ttgtcaaaat ccttcttggg aaattccttg taggacgcca gatacttgac
     1441 accgctgact tccagattaa acttgctgtc cttagtttcg taaaagtatt tataagtttc
     1501 tttaatttca gggaaatcag actcttccac ttttctgggt ctgagttttc cctcttccag
     1561 aatcatcgga acggaagagt caggaaaggc aacaatctct cccttgctgt ttgtaatgta
     1621 aataatgctt ttcttgccga tattctggtt tttcagttca tctttcagat tcatcagaaa
     1681 ctcggaaatt gccacgagtg gaatatccag tccgaaaaca cccttgaatt ttccgtcctt
     1741 gtctctgacc ggggaagaag ctgttactcc aggaacttta tccgtcgcaa aaatataaac
     1801 atcactccag aaaagtttgc cctctctttt ggctccctga taccacggtc tttctctggg
     1861 atcatacttg tcttcagtat ccagatcttc ctttatgatt tttccttcca ggtcccggcg
     1921 tctccatttg acagtcactt tgcctttttt acgggtaact actttagtgg aaatggtgcc
     1981 gtcctgctct cttctctgca tcaggaagtc gccattgtca tttccgattt caaaattttt
     2041 cagctgaggg tgaagtttga gaatggcaat catcagaccg tccagtctag gattgttgtc
     2101 aagagtttcc accccttcca ctgtgatttt tgagctgagt tcagtcatat ccactgcagt
     2161 catcagataa tttttcgcat tctgaaaaac tctctgattg atctgatcga ttacgtcttc
     2221 cgagagatcc agaatggcat tt
//
LOCUS       DAOQGJ010000525         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31952_length_2241_cov_3.162397, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000525 DAOQGJ010000000
VERSION     DAOQGJ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31952_length_2241_cov_3.162397"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="PK683_09140"
     CDS             complement(<1..1550)
                     /locus_tag="PK683_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08654.1"
                     /translation="MSYTIQKLKQNYKMQLALGKTIPQSELGFPIPLSTGNPLYDASL
                     TEKISATAMMDESEHLARAWELMRNSSSEELEKLSRFSKGILNDFRLLGAVRAASVGR
                     EDLENQFLSSLPWGWKLSYPSAVISPASQFFTGWKKDLKFRLTEEISFPKLREVYGNL
                     IQKAGGTAIMKYRGKIQEAMALLKYRPEGEKEKAVHNFCFYNGKGIQDIPELEPIGTF
                     LKCRNALLSGSIPEFLNILENSPGEIPLTSYMGLLGSFRIRLKENTQPYIERLRNYAV
                     RSATAAEAVLRLREWGTWLSDEQIELLSGKLRTSAETGIHIPFFKITKAFINSPDRIR
                     KKLLKPLYIPMMKQFSSSTADLLGKDAVCSFFMPGNMVHSMSYLLYAVAASGMKTRLV
                     LLYRDGTEEVQFSPDEIALHLTDEPRQFQEWLLAQIGGLASYQTYTYHYPSLAREIGK
                     LNPADPVVLDLPFSGSREVLSALLPFEQVFNLNSLCGAPGEICMAYEYYLDFAFLSRN
                     WHYGSWTR"
     gene            complement(1870..2076)
                     /locus_tag="PK683_09145"
     CDS             complement(1870..2076)
                     /locus_tag="PK683_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08655.1"
                     /translation="MPKKIQESKKVGRKKKKEEFPEEENWRKPESEMDRPEDSLAYQV
                     AMEIQERILNGEKISDYPDILKDL"
BASE COUNT          583 a          513 c          476 g          669 t
ORIGIN      
        1 aatcttgtcc aggaaccgta gtgccagttt ctggagagaa atgcaaaatc cagataatat
       61 tcgtaagcca tacagatttc tccaggagca ccgcacagag aattcagatt gaatacctgc
      121 tcaaatggaa gaagagcaga aagcacttct ctgcttcctg aaaacggcag atccagaaca
      181 actgggtcag ccggattcag ctttccgatt tcccttgcaa gggaaggata atgataagta
      241 taagtctgat aggatgccag tccgcctatc tgtgcaagaa gccattcctg aaactgtctg
      301 ggctcatcag tcagatggag cgcaatttca tccggagaaa actgtacttc ctcggttccg
      361 tctctgtaaa gaagcacaag tctggttttc attccggatg ctgcaacagc atataataag
      421 taactcatag aatgtaccat attgcccggc ataaaaaaac tgcagacagc atcctttcct
      481 aggagatctg ccgtggagga agagaactgt ttcatcatcg ggatgtaaag aggtttcagc
      541 agctttttcc tgatcctgtc aggagaattg atgaatgctt tggtgatttt gaaaaatgga
      601 atatgtattc ctgtttctgc acttgtcctg agttttccgg aaagaagttc gatctgttca
      661 tcactgagcc aggtgcccca ttccctcagc cgcagtactg cctcagccgc agttgctgat
      721 ctgactgcat aatttcgaag cctttcaata taaggctgag tgttctcttt cagacggatt
      781 ctgaaactgc cgagaagtcc catataggaa gtgaggggaa tttctcctgg actgttttcg
      841 agtatattca gaaattccgg tatgctgccc gacagaagcg catttctgca tttcaggaat
      901 gttcctatcg gttccagttc tggtatatcc tgtattcctt ttccattata aaaacagaaa
      961 ttgtggactg ccttttcctt ctccccttca ggtctgtatt tcagaagagc catcgcttcc
     1021 tgaatttttc cgcgatattt cataatggca gttccgcctg ctttctgaat gaggttcccg
     1081 taaacttccc tgagtttagg aaagctgatc tcctcagtca gacggaattt cagatccttt
     1141 ttccatcctg taaagaactg gcttgcagga gatatgactg cagacgggta ggaaagtttc
     1201 cagccccagg gcagagaaga aagaaactga ttttccagat cttcccttcc gacagaagca
     1261 gcgcgaactg cccctagaag acggaaatca ttcaggatgc ccttggaaaa acgcgaaagc
     1321 ttttccagtt cctcactcga agaattgcgc atcagctccc atgcgcgggc aagatgttct
     1381 gattcgtcca tcattgcagt tgctgagatt ttttctgtaa gacttgcatc atacaaaggg
     1441 tttcctgtag acagcggaat cggaaatcct agttcactct gcggaattgt ttttcccagt
     1501 gccagctgca tcttataatt ctgtttcagt ttctggatag tataactcat atattttttt
     1561 ccgtgtgagg ccgccgggta caagggtccg gcggcgtttg gagccgttcg agggcgtctg
     1621 aacgctctgc gttccaaagg ctgcagagca gtgccaatat actgaagacc tgctttactg
     1681 caactgaaaa aatctttgat ctcattgttc cacaaagact gagtcttcct gcaataagta
     1741 gagaaatttt ataataatga ctgttaagaa aatactgcag gcgcccagcc tgcaaaataa
     1801 gcaagttcac agccggaatg tctgtgatac tttcccaaag gaatttttcc tgctaaatcc
     1861 aaggacacct catagatcct tcagaatgtc cggatagtcg gaaatttttt ccccgttcag
     1921 aattctttcc tgtatctcca ttgccacctg gtatgcgaga ctgtcttcag gacgatccat
     1981 ttccgattca ggttttctcc agttttcttc ctctggaaat tcttctttct ttttctttct
     2041 gcccactttc tttgattcct gtattttctt aggcatatct tttctccttt tgttctgctt
     2101 ctgcagactc attcataatg gttttgaaat tggaaaagtg tcagcaggag gatgaatata
     2161 ctaattttca aaagtctttg cgcatttact cttcctcgac tcaatagaat tagggctgtc
     2221 agcagaaaaa acgcaattat t
//
LOCUS       DAOQGJ010000526         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_31965_length_2240_cov_4.301602, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000526 DAOQGJ010000000
VERSION     DAOQGJ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31965_length_2240_cov_4.301602"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PK683_09150"
     CDS             complement(<1..629)
                     /locus_tag="PK683_09150"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH08656.1"
                     /translation="MKKLPIGIQTFRDIIENNYIYVDKTKHIHQLIESGKGTYFLSRP
                     RRFGKSLLISTLAEIFLGNRELFKGLYIADSDYSWKKHPVIRLDFSKQKAETREALIS
                     YLIGELELNAKKYSITLTKAEYYSQFEELILKLSESGKVVILIDEYDKPIIDWLDRPE
                     QAKEMRSVLKGFFSVLKGNDANIRFLFLTGVSKFSPLSSPFKGDIGGSD"
     gene            complement(717..1472)
                     /locus_tag="PK683_09155"
     CDS             complement(717..1472)
                     /locus_tag="PK683_09155"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNH08657.1"
                     /translation="MKGESAYQAYLESDHCTTKKRKDESWTEALERMDRRREEFIPQF
                     KNAWDEFYWRSLKQGRKKSDRNISSLRPLSEEQVQKIEQNLGGIRLPKDYRDFLIKIG
                     YSESLSLYSLDADERPKYSAARVFPLVKSYHEKTDSEYEKLKLNPVDHLEQSMSPSLK
                     HIAGSYYQRQKMSVWDGQIVLYSGAVSLIVTGEMRGRVWAYNDFDRYILEPAEEADHS
                     CSFLNVLKNENREPVDFIDYVNLFLINEFRDIS"
     gene            complement(1469..2005)
                     /locus_tag="PK683_09160"
     CDS             complement(1469..2005)
                     /locus_tag="PK683_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNH08658.1"
                     /translation="MERYILLLRGINVGGHHKLPMKDLKLEMEKLGMHCIATILNSGN
                     AVFEYKKEKIPKIEELISSHLEKVFGFPVPSIVRTQKEIQSASEEDCFDKVDLKRERH
                     QYISFSKNKLKSEIKLPWKSKDESFEILKVNDKEIFSILYLDKGKSVKGMEELEKIFG
                     KDLTTRNLNTVQKLSVIK"
     gene            complement(2008..>2240)
                     /locus_tag="PK683_09165"
     CDS             complement(2008..>2240)
                     /locus_tag="PK683_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNH08659.1"
                     /translation="CRFKNCTHENEPGCAVQEALSEGELDEERYAHYLKLLREEKYME
                     SKISEKNALVKKNNDKKLAKMIRERLKDKGRK"
BASE COUNT          563 a          525 c          379 g          773 t
ORIGIN      
        1 aaatcactgc cccctatgtc ccccttaaag ggggatgaaa ggggggaaaa tttgctcact
       61 cctgtcagaa acagaaaacg gatgtttgca tcgttaccct tcagaacaga aaaaaaacct
      121 ttcagaactg agcgcatttc ttttgcctgc tcaggtctgt ccagccagtc aatgatcggc
      181 ttgtcatact catctatcag aattacaact tttccagact cgctcaattt taatataagt
      241 tcctcaaact gggaatagta ttcagcctta gtaagtgtta tactgtattt ttttgcattt
      301 aattccagtt ctccgatcag ataactgatc agtgcctctc tagtctccgc cttctgtttg
      361 ctgaagtcaa ggcgtatcac tggatgtttt ttccagctgt agtctgaatc tgctatatac
      421 agccccttga aaagctctct gtttcccaga aagatttctg cgagtgttga aatcagaaga
      481 cttttgccga accgacgggg acgggagaga aagtatgtgc ctttcccgga ttcaatcaac
      541 tggtgaatat gctttgtctt atcaacataa atataattat tctcgataat atcccgaaat
      601 gtctgaatcc cgatcggcag tttcttcata agactaatct cagggtctga ttcactttgg
      661 caagaagaat cctgtcttaa gttctgtgct tccgcatttt gcagtttcga aatttatcag
      721 gatatatccc tgaattcatt tattaaaaaa agatttacat aatcaataaa atcaacaggc
      781 tctctgtttt catttttcag tacattcagg aaactgcagg aatggtctgc ttcttctgca
      841 ggctccagga tgtatctgtc aaaatcattg tatgcccaga cccgtcctct catctcgcct
      901 gtcacaatca gactgactgc acctgaatac agaacaatct ggccgtccca aacactcatt
      961 ttctgtctct gataataact tcctgcaata tgttttaatg atggagacat ggactgttcc
     1021 agatgatcca ctggattcag tttaagcttc tcatactcac tgtccgtttt ttcatgatag
     1081 gatttcacca agggaaaaac tcttgctgca gaatatttcg gacgctcatc tgcatccagt
     1141 gaatacagcg aaagactctc tgaatatcca attttaatca gaaaatctct gtaatctttc
     1201 ggaagacgga ttcctcccag attctgttca attttctgca cttgttcttc actgagaggt
     1261 cttaatgatg atatattccg atccgacttt tttcttccct gtttcagact tctccagtaa
     1321 aactcatccc aggcgttctt aaactgggga atgaattctt cccttctccg gtccattctt
     1381 tccagtgcct cagtccagga ttcatctttt ctttttttgg tggtgcaatg gtcagactcc
     1441 agataggcct gatatgctga ttctcctttc attttatcac actgagcttc tgaactgtat
     1501 tcagatttct tgtcgtgaga tccttgccga aaattttctc cagctcctcc atccctttca
     1561 ctgattttcc tttgtcgaga tacaatatac tgaaaatttc cttatcattc accttcagaa
     1621 tttcgaacga ttcatccttg gatttccatg gaagttttat ttcagacttg agcttattct
     1681 tcgaaaaaga aatatactga tgcctctctc tttttaagtc caccttatca aagcagtcct
     1741 cctccgaggc tgactgtatt tccttctggg tccttacaat ggaaggtaca gggaaaccga
     1801 atactttttc cagatgagaa gaaatcagtt cttctatttt cggaattttc tcctttttat
     1861 attcaaagac tgcatttcct gaattcagga ttgtcgcaat acagtgcatt cccagttttt
     1921 ccatttccag cttcaggtct ttcatcggaa gtttatgatg tccgcccaca ttgatccctc
     1981 gcagaagcag tatatatctt tccattccta tttccttccc ttgtctttca gtctttcacg
     2041 gatcattttg gctaattttt tatcattatt tttcttcact aatgcatttt tttcactgat
     2101 cttggattcc atatatttct cttctctgag aagtttcaga taatgcgcat atctctcctc
     2161 gtccagttct ccttccgaaa gagcctcctg gacggcacag ccaggttcat tttcatgagt
     2221 gcagtttttg aatctgcatt
//
LOCUS       DAOQGJ010000527         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32036_length_2237_cov_3.225481, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000527 DAOQGJ010000000
VERSION     DAOQGJ010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32036_length_2237_cov_3.225481"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PK683_09170"
     CDS             complement(<1..1247)
                     /locus_tag="PK683_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH08660.1"
                     /translation="MAKSKVYEPKAEFVKNTNLNEKAYKKMYAESVKNPESFWKKQAM
                     ERITWFKPFTKVLSHKFSEGKVKWFEGGKLNVSYNCLDRHLNTSVKNKAAIIWEGDNP
                     NESKTYTYQQLHREVCRFANIMKKMGVKNGDRVLIYLPMIPELAISALACTRIGATHS
                     IVFGGFSPEALTGRIDDCKPSLIITSDGGFRGGKIIDLKKNVDEATEHAETKVKNIIV
                     VKRTHQSGNFHWKEGRDHWWNDLQMDETISDECEPYKANSEDPLFILYTSGSTGKPKG
                     VLHTTAGYLLGANLSFHYIFDHKDSDTYWCTADLGWITGHSYILYGPLSNGATSLMFE
                     GVPTYPDAGRFWDVIDKYKVNVFYTAPTAIRALAREGLEPLKGKSLKSLRLLGTVGEP
                     INPEAWEWYNVNIGKKNCPIVDT"
     gene            1669..2226
                     /locus_tag="PK683_09175"
     CDS             1669..2226
                     /locus_tag="PK683_09175"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HNH08661.1"
                     /translation="MTAEHMNFEPLNEGYRTEIEFKRGGVSTVLVPASLLKSFKIKCR
                     KHKGKAEYFSFLMKRFGIVLKCHTMNPEGLKTLFQKKNQKLKKVNFRPLDEDWAELGT
                     FSVASGRSRCLLFVILLSFDSGNLTSVLKNAGIVIHSPLPPGKSWELLSTFGANRAAG
                     HCIRAFGRKISEREVHEVLEKNINT"
BASE COUNT          584 a          471 c          489 g          693 t
ORIGIN      
        1 aatgtatcca cgataggaca gttcttcttc ccgatattga cattgtacca ttcccaggct
       61 tccgggttga taggttctcc tacggttccc agaaggcgga gtgatttcag tgattttcct
      121 ttcagcggtt ccaatccttc ccgtgcaagt gcccttatcg cagtcggtgc tgtataaaaa
      181 acattcactt tgtatttgtc gatcacatcc cagaacctgc ctgcatcagg atatgtagga
      241 actccttcaa acatcagaga ggtcgcccca ttggaaagag ggccatagag gatatagctg
      301 tgaccagtga tccagcccag gtcggcagtg caccagtagg tgtcggaatc cttatgatca
      361 aaaatataat ggaaggagag gtttgcacct aagagatagc ctgcggttgt atgaagcact
      421 cctttgggtt tgcctgtaga acctgatgta taaagaatga agaggggatc ttctgagttt
      481 gctttatagg gctcacattc atctgagatt gtctcgtcca tctgcagatc gttccaccag
      541 tgatcccttc cttctttcca atgaaaattg ccggactgat gagttctttt gactacgatg
      601 atatttttca cttttgtctc tgcgtgttct gtcgcctcgt ctacattctt tttgagatca
      661 atgattttgc ctccgcgaaa tccgccgtcg cttgtgatga tgagtgaggg cttgcagtcg
      721 tcaatcctcc cggtcagtgc ttcaggggaa aatcctccga aaactatgga atgagtggct
      781 ccgatcctag tgcatgccag agcagaaatt gccagttcag gaatcatcgg aagatagatg
      841 agcaccctgt ctccgttttt gactcccatc tttttcataa tattggcaaa tctgcacact
      901 tctctgtgga gctgctggta tgtgtatgtc tttgattcgt tgggattgtc accttcccag
      961 ataattgcgg ctttgttttt gaccgaagtg ttcagatgcc tgtcgagaca gttgtatgag
     1021 acattgagtt tgcctccttc aaaccatttg acctttcctt cggaaaattt atgactgagc
     1081 acttttgtga acggcttaaa ccaggtgatt ctttccatag cctgcttttt ccagaagctt
     1141 tccggatttt taactgattc cgcatacatc ttcttgtatg ctttttcatt cagatttgta
     1201 ttttttacaa attcagcctt tggttcgtag accttggatt ttgccattcg gttctcctgc
     1261 gtccaattga gttcaggaac cattattctg ctctttaatt ttcgtccaga ttatttcttt
     1321 actctctgct attcttctcc ccatacaatt gtacattctg cataaaggaa aggacgaacg
     1381 atctgtccgg agattctgtt ccaggcagcg gatcgaaaaa tctgtaatat atttgggaaa
     1441 ttgggaaaac tgattttgca gatcataatg aaaaaataaa tctcactgag tctgattttc
     1501 ctgattggaa tgcctgcagc agcaatcaag ctgttccttg taggaactcc ctcagatcta
     1561 tttgagaaga attgcatcta agatataaaa ttttccgaaa ggtgcaaaaa tagatttttt
     1621 ttgtcccgac ctctccattt ttaaacaggt acagtcagag gaaatctgat gactgcagaa
     1681 catatgaatt ttgaaccttt gaatgaaggc tacagaactg aaattgaatt caaacgagga
     1741 ggggtctcca ccgtacttgt tccggcttcc ctgctgaaaa gttttaaaat aaagtgcaga
     1801 aagcataaag gtaaagcgga atacttttca ttcttgatga agcggtttgg aattgtactg
     1861 aagtgtcaca cgatgaatcc ggaaggactg aagactctgt ttcaaaagaa aaatcaaaaa
     1921 ttaaaaaagg tgaattttcg gcctttggat gaggattggg ccgaactcgg taccttttcc
     1981 gttgcttcag gaaggagcag atgccttctt tttgtgattc tgctttcttt tgattctgga
     2041 aatctaactt cggtcctaaa aaacgcgggt atcgtgatcc attcacccct gcctccagga
     2101 aaatcgtggg aactcctcag cactttcgga gcaaataggg ctgcgggaca ctgtattaga
     2161 gcatttgggc gaaaaatctc agagagggaa gtgcatgaag ttctggaaaa gaacataaac
     2221 acgtagaaag ttctaaa
//
LOCUS       DAOQGJ010000528         2236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32055_length_2236_cov_3.246217, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000528 DAOQGJ010000000
VERSION     DAOQGJ010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32055_length_2236_cov_3.246217"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PK683_09180"
     CDS             <1..819
                     /locus_tag="PK683_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08662.1"
                     /translation="KSSQKIKWKSYTVTLKNEGPDSEIWKKFSLKIKTKNKKTVFQKK
                     LESASLQLAGIYRSGGQEFLHLSGYTGGESGCCSTEEFFFETPEGIKSISFEQLAYNV
                     LKVTDMDKDKDEEIMTHNERYYGLSLDDSLLGGDGKCSVLSYQLYTSGFANEVFPIYS
                     KLLKVKDGNYSVSDVTFLENYRNALKPYLRKAENSLKASKGKTLNPISGDMMQGFLQY
                     WYYMKMIGRAEEAEKKIADSGIQVVFSCESGSKSYPLRDVIVKNAKKLLEEEKK"
     gene            complement(820..>2236)
                     /locus_tag="PK683_09185"
     CDS             complement(820..>2236)
                     /locus_tag="PK683_09185"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNH08663.1"
                     /translation="NDPVTKYIPELKMKKRNPEDRIPTVRNLLTHHSGLPSDIMNGFL
                     TDQKTEEHTEKFRSLGKILENEHMANQPEKTHSYSNLAFGLLGLVIERAAGKSLDSYM
                     REKIFLPIGMKSTSYVFLPNESYSKAYFGKEERDMLKIRDLGAGSVSTNLDDFSNFLL
                     FINSEGRAGNGEVLKKSSFLEMTEPQNKNIVFDFGQSTGLPFIFSVPFNDKIIIGHDG
                     YLPPFVSSFLFSPKEKIAVFTSYNTALSSPSEGTPEIMEEFLEAVSGEKKKPNDNDKI
                     ITIEKQTLQKFDGKYAAIPGSFEFTADEGGYRTDKMLYLYPKSDGTFGIKVRILGINL
                     SVEALKKLNVQFAEHSGEKVFSMNYGNFSVMKGVQYTPKKLTDVWKKRKGKYEIINHS
                     SYKVFQNPEISLNEDDGMIVFSTQLDIGAKGAFQISFLPETDTTARTPGLGRYAGEVM
                     YITEENGKEILNYDGYKFRKK"
BASE COUNT          646 a          435 c          459 g          696 t
ORIGIN      
        1 aagtccagtc agaaaataaa atggaaatct tatactgtca cacttaagaa tgaaggtcct
       61 gattcggaaa tctggaaaaa gttcagtctg aaaatcaaaa caaagaataa aaagacagtg
      121 tttcagaaaa agctggaatc tgcttcactg cagctggcag gaatctacag atcgggcggg
      181 caggagtttc ttcatctttc agggtataca gggggagaaa gcggatgctg ctccactgaa
      241 gaatttttct ttgaaacacc ggaagggatc aagtccattt catttgaaca gcttgcatat
      301 aacgtgctga aagtcacgga tatggacaag gacaaagatg aagaaatcat gactcacaat
      361 gaaagatact acgggttaag tctggatgat tcgcttttgg gaggagacgg aaaatgttct
      421 gttctaagtt accagctgta cacttccggt tttgcaaacg aagtgtttcc gatttattca
      481 aagcttctta aggtaaagga tggaaattat tccgtaagtg atgttacttt tctggaaaac
      541 tacaggaatg ctttgaagcc ctatctgaga aaggcggaaa actcactgaa ggcatccaag
      601 ggaaaaacac tgaacccgat ctccggagat atgatgcagg gttttcttca gtactggtat
      661 tatatgaaga tgattggaag agctgaggaa gcggaaaaga aaattgccga ttcaggaata
      721 caggtcgtat tttcctgcga gagcggatca aaaagctatc cgctcaggga tgtcatcgta
      781 aaaaatgcaa aaaaacttct ggaagaggaa aagaaataat tattttttcc tgaatttgta
      841 tccgtcatag ttcagaattt cttttccgtt ttcctctgtt atgtacatta cctcgccggc
      901 atatcttccc agccccggtg ttcttgccgt tgtgtctgtt tccggaagaa aggaaatctg
      961 aaaagcccct tttgcaccga tgtcaagctg agtactgaat acaatcattc cgtcatcttc
     1021 attcagactg atttcaggat tttgaaacac tttataactg gaatgattga taatctcata
     1081 ttttcctttt ctttttttcc agacatcggt cagttttttc ggagtgtact gaactccttt
     1141 cattacactg aagtttccgt aattcatact gaataccttt tcaccggaat gttcggcaaa
     1201 ctggacattc agtttcttga gtgcttccac gctcaaattt attccgagta ttcgtacttt
     1261 gattccgaaa gtcccgtccg atttcggata cagatagagc atcttatctg tcctgtaccc
     1321 tccttcatcc gcagtaaatt caaatgatcc gggaatggca gcatattttc cgtcaaattt
     1381 ctggagagtc tgtttttcta ttgtgattat tttatcatta tcattcggtt ttttcttttc
     1441 tcccgaaact gcctcaagaa attcttccat tatttccgga gttccttctg aaggtgaact
     1501 cagggcagta ttgtagcttg taaatactgc aattttttcc ttcggggaaa acaggaaact
     1561 cgagacgaaa ggaggaagat acccgtcatg accaataatg attttatcat taaaaggaac
     1621 tgagaatatg aaaggcaggc cggtgctctg accgaaatca aaaacaatat ttttattctg
     1681 aggttcagtc atttccagaa agctgctttt tttaaggact tctccgtttc ctgctctgcc
     1741 ctcactgttt atgaacagaa gaaaattgga aaaatcatcc aggttggtgc tgactgaacc
     1801 tgctcccaaa tctctgattt tcagcatatc tctttcttct tttccaaagt aagcctttga
     1861 ataagattca ttcgggagaa aaacataact tgtggatttc attcctatcg gaaggaaaat
     1921 cttttctctc atataagaat cgagagattt tccggctgct ctttcgatta caagtccaag
     1981 aagtccgaaa gccagattgg aataggaatg agtcttctca ggctgattgg ccatatgttc
     2041 attttcaaga attttgccca aagatctgaa tttttccgta tgttcctctg tcttctgatc
     2101 agtgagaaat ccgttcataa tgtcggaggg aagtcctgaa tgatgagtca gcagatttct
     2161 tacagtggga atcctgtctt cgggatttct tttcttcatt ttcagttccg ggatatattt
     2221 agtgaccgga tcattc
//
LOCUS       DAOQGJ010000529         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32084_length_2235_cov_2.856881, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000529 DAOQGJ010000000
VERSION     DAOQGJ010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32084_length_2235_cov_2.856881"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="PK683_09190"
     CDS             complement(<1..1796)
                     /locus_tag="PK683_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH08664.1"
                     /translation="MKDIFILLILTVILFFFQGISAEESKVFYYFTPDRILPVSEMQC
                     RPGFQISSEKDEENAFEWFKIKLPGYWKFSSMPSAKSHICRFHFKAENHQNVRYSINL
                     LNVINSHEIYLNSEKIGGKGSFDTQGNLIQPNPSPALYDIPSHLLKDENVIMLRIVDS
                     ASGGGILEMPSFGESGELERKFYLKMIRDGGFAIFFITIAIYHIFIYAVIRKNKELLV
                     FGIIALCGGIGALGYYKLTYVIVPNFYFHFYALHISHQGGFLLFPFFISRLFSLRLNW
                     VSVFMISVHSVNLGILFLTLFSYAVREFFRSYVVMFTVTVMYVALSFLILQTVFSVRK
                     RMTLENKIISVGLVLCAISAFQFVFSFNGIVPVTHFLGESFLVLTLCTSVSIAVKYNA
                     AQEELIQMKSDYNAELEKEVALKSEVLKQTNTDLQRSNMEKERLLTVLAHDLRSPLNL
                     LQDSINVLQYDSLSGRDFKRIIREISLNLKRNKYVLENLLVWSSRKLASDPAVKQRTD
                     ISIIVMETVEFFKSDLKKKKIRIDHSFNVPVYCRTDRNTVRLVLRNLISNSIKFSGKK
                     SVIHAGFSMKLKENKVRISVQDQGSGLSKEAL"
     gene            complement(1814..>2235)
                     /locus_tag="PK683_09195"
     CDS             complement(1814..>2235)
                     /locus_tag="PK683_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08665.1"
                     /translation="EKNSDLKGQSRINRIRLAECYLYRDDLVLPEKKDSIFRREELLQ
                     RIKDNYALAAFIFSSEITEIEKITPNEKLLGLWYYEWGLSEMMIQKREKAVGLFLKSA
                     QIDPSISESTYYAAFLSLQLGREKDADRIWNRERKKK"
BASE COUNT          659 a          481 c          454 g          641 t
ORIGIN      
        1 ttcaatgctt ccttggaaag tcctgatccc tgatcctgca cagaaattct gaccttgttt
       61 tccttcagct tcattgaaaa acctgcatgg atcacagatt ttttcccgga aaacttgatg
      121 gaattcgaaa taagatttct gagcacaaga cggactgtgt tcctgtctgt tctgcagtat
      181 acaggcacat tgaaactgtg gtcaattcgt atttttttct ttttcagatc ggatttaaaa
      241 aactcgactg tttccattac aatgatgctt atgtccgttc tctgcttcac cgcaggatcg
      301 gatgcaagtt ttcttgacga ccatacaaga aggttttcaa ggacatactt gttccttttc
      361 agattcagtg aaatctccct gatgattctc ttgaaatctc tgccgctgag cgaatcatac
      421 tgaagcacgt tgatcgaatc ctgaagaaga ttgagcggtg atcttaaatc atgtgcaaga
      481 acggtgagaa gtctttcctt ttccatattg gaccgctgca gatcagtatt tgtctgcttc
      541 agaacttcag acttcagagc cacctccttt tccagttctg cattgtagtc ggacttcatc
      601 tgtataagct cttcctgtgc agcattgtat tttactgcaa tggaaacaga agtgcagaga
      661 gtaagaacaa gaaaactctc acctagaaaa tgagtcaccg gaacgatccc gttgaagctg
      721 aaaacaaact gaaacgcaga tatcgcgcag agaacaagcc cgacggaaat aatcttgttt
      781 tcgagagtca ttctttttct gacagagaat acggtctgca ggatcagaaa agaaagcgca
      841 acgtacatta cagtcacggt gaacatgacg acataggatc tgaaaaattc tctgactgca
      901 taggagaaca gtgtaaggaa aagtatcccc agattgaccg aatgaaccga aatcataaaa
      961 acggaaaccc agttcagtct taaactgaag agcctggata tgaagaaagg gaaaagaagg
     1021 aatcccccct gatgtgaaat atgcagagca tagaagtgaa aataaaaatt gggaacaatt
     1081 acataagtaa gcttataata tccgagtgca cctattcctc cgcagagtgc tattatcccg
     1141 aagacaagca gctccttgtt ttttctgatg actgcataaa taaaaatatg atatatcgca
     1201 attgtaataa aaaatatcgc aaaaccgccg tccctgatca tcttcagata gaactttctt
     1261 tccaattccc ccgattcgcc gaatgaaggc atctcaagta tccctcctcc cgaagcagag
     1321 tccactatcc ggagcataat tacgttttcg tctttcagga gatgagaagg gatatcgtat
     1381 aatgcaggtg aggggttcgg ctgaatcaga ttcccctgag tgtcaaaact gcccttccct
     1441 ccgatctttt ccgaattcag atatatttcg tgagaattga tgacgttgag aagattgatg
     1501 gaataccgaa cgttctgatg attctctgcc ttgaagtgaa atcggcagat atgcgatttt
     1561 gctgaaggca ttgatgaaaa tttccagtag ccgggaagtt tgatcttgaa ccactcgaaa
     1621 gcattttctt catctttctc ggaggagatc tgaaacccag gccggcactg catttcggag
     1681 acaggcagaa tcctgtccgg agtaaaataa taaaagactt tactttcttc ggcagaaatt
     1741 ccctgaaaaa aaaataaaat gacggtcagg atcagcagga taaaaatgtc tttcactgcc
     1801 cggcccataa tgcctacttc ttctttcgtt ctctgttcca gattctgtcc gcatcctttt
     1861 ctctcccaag ctgaagggac aggaaggcgg catagtaggt gctttctgaa atggaagggt
     1921 caatctgtgc agatttcagg aacagtccga ctgccttttc ccttttctgg atcatcattt
     1981 cagaaagtcc ccattcatag taccaaagtc caagaagttt ttcattcgga gtaatctttt
     2041 caatttcagt gatttcagag gaaaaaataa atgccgccag tgcataattg tccttgatcc
     2101 tctgaagaag ttcttcccgt ctgaatatgc tgtctttttt ttcaggaagg acaagatcat
     2161 ctctgtagag ataacattca gcaagcctga ttctgttgat tctgctctga cctttcagat
     2221 cagaattttt ttcaa
//
LOCUS       DAOQGJ010000530         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32088_length_2235_cov_2.494495, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000530 DAOQGJ010000000
VERSION     DAOQGJ010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32088_length_2235_cov_2.494495"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1287)
                     /locus_tag="PK683_09200"
     CDS             complement(49..1287)
                     /locus_tag="PK683_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08666.1"
                     /translation="MLLEEEPSVDNNSFFAKTKRLILGLSLSVLVVMLILMLIPNDAE
                     QNFYNIITGKSSLHAGKVGSEDISEEYFQASRRICSNMFKSDRQTEENIINCAFETAK
                     RFKISRIISEAVGFSISEQKIKADIWEQAKMTHKETAVGAGYSEEEKLSAEEIYKNNL
                     LSIPMSFRKDMILADSVMERFLLASPEPSEDEKKIKSEMKGLKLSLRYAFFSETDYLN
                     SINDLPVSDEEVKKDYEESVKSGNLKNAKGEVPSLDERKPLIINKLRSEKKQQKLAEM
                     KANLQKLKGSDKTTLTEIAALAGSKIQEVKDLPISELNRMSEKPNSQPWKFVTKAAFQ
                     KDLTEIPFGQGKIGGPYQEGDKQVFVEFSSMKIEEIKDKVADDNSKNIEKYKAYTFIG
                     EAVQSISSVYPVYRNIKFQK"
     gene            1346..1975
                     /locus_tag="PK683_09205"
     CDS             1346..1975
                     /locus_tag="PK683_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08667.1"
                     /translation="MKKDYLLAVTGIVPLFFLFYLFNYVSFDKRKDVFNSSVLIEDSA
                     VSELKVSSSVKFTGKLTAENRNMYKEYPLAAFEKKVKEGDRSRWVLESSQVQKLELKS
                     DKFSKPFFITFADDYIPCGNTVDVRENETDRKKRLLGLRRDSVLTGYGKIQSLEPLVI
                     ESGHTACAETLKEYSDKLGNKSLIMLIVGLVIAVPSLFLVYLGLFRKLQ"
     gene            2027..>2235
                     /locus_tag="PK683_09210"
     CDS             2027..>2235
                     /locus_tag="PK683_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08668.1"
                     /translation="MKKKLTLFCFLFSCALHSVPVSLMGEWELKEGKNLNETGLEGWI
                     KSTSLPLNFVQLKSSSGCKLCTVTF"
BASE COUNT          598 a          449 c          439 g          749 t
ORIGIN      
        1 ctgcttcaat aaaagggtta acaaactctg ccctgacttc catattacct acttttgaaa
       61 tttaatattt cgataaaccg gatatacact gctgatggat tgtaccgctt cacctatgaa
      121 tgtatatgcc ttatattttt cgatgttctt actgttatca tcggcaactt tgtccttgat
      181 ttcttcaatt ttcattgaag aaaactctac aaaaacctgc ttgtcgcctt cctgatatgg
      241 accgccgatc ttgccttgtc caaatggtat ctcagtcaaa tctttctgaa atgcagcctt
      301 tgtaacgaat ttccaaggct gagaattcgg cttctccgac attctgttca gttcagagat
      361 agggagatct ttcacttcct gtattttcga gcctgcaagt gctgcaattt ctgtcagagt
      421 tgttttgtca gaacctttca gtttctggag atttgccttc atttctgcaa gcttctgctg
      481 tttcttttct gatctaagtt tattgattat gagaggtttt ctttcatcca gcgaaggaac
      541 ttcacccttt gcattcttca ggtttcctga cttgacggat tcttcgtagt cttttttgac
      601 ttcttcatca gagacaggaa gatcattgat ggaattcagg taatcagttt cagaaaaaaa
      661 tgcatatctc agactcagtt tcagaccttt catctcagac tttatttttt tctcgtcttc
      721 gctcggttca ggacttgcga ggaggaatct ttccataaca gagtcggcca gaatcatatc
      781 ctttctgaaa ctcatcggga tggaaagaag attgtttttg tagatttcct ctgcagaaag
      841 tttttcctcc tctgaataac cggcgccgac tgcagtttcc ttatgggtca tttttgcctg
      901 ttcccaaatg tctgccttga ttttctgttc cgatatgctg aagcctaccg cttcagagat
      961 gattctggat atcttgaatc tttttgcggt ttcaaatgca cagttgataa tattttcttc
     1021 tgtctgtctg tctgatttga acatattgga gcaaattctt ctggatgcct ggaaatactc
     1081 ttcagaaata tcttcagaac ctacctttcc ggcatgaagg ctcgattttc cggtaataat
     1141 attataaaaa ttctgttctg catcatttgg aatgagcatc agaatcagca taactaccag
     1201 tacggataaa gaaagaccca agatcagtct tttggtcttt gcgaaaaaac tattattgtc
     1261 tacagacggt tcttcttcta aaagcataga ttaagcataa ttttttttgc tttgagcaaa
     1321 tgtaaacagt tttaaaactt gctttatgaa aaaagattat ctgctcgcag tgacaggaat
     1381 cgtccctctg ttttttctct tctacctgtt caattacgtt tcctttgaca aacgaaagga
     1441 cgttttcaat tcttccgtcc tgattgaaga cagtgcagtt tccgaattga aggtcagttc
     1501 ctctgtcaag tttactggaa aactgactgc cgaaaacagg aatatgtata aggaatatcc
     1561 tctggctgcc tttgaaaaaa aagtcaaaga aggggacaga tccagatggg tactggagtc
     1621 gagccaggtt cagaaacttg aattaaaatc agacaagttt tccaagccgt tttttatcac
     1681 atttgcagac gattatattc cctgcggaaa tactgtggat gtaagggaaa atgaaacaga
     1741 cagaaaaaaa agacttctgg gtctccgaag ggattctgtt cttaccggat atggaaaaat
     1801 acagtctctt gagcctctgg tcattgaaag cggccatact gcctgtgcag agactcttaa
     1861 ggaatattcg gacaagctgg gaaacaagag tctgataatg ctgatcgtag gacttgtcat
     1921 cgcagtgccc tccctttttc tggtgtatct ggggctcttc agaaaattgc agtaggatgc
     1981 aatttattca ttgaaaaaaa atgagtgctt catccaatat cggtttgtga aaaaaaagct
     2041 tactctgttc tgtttcctgt tttcctgtgc tctgcattct gtgcccgttt ctcttatggg
     2101 agaatgggaa ctgaaggagg ggaaaaatct gaatgagacg ggtctggaag gatggatcaa
     2161 aagtacatcc ttacctctga attttgttca gcttaaatct tcctccggat gtaaattatg
     2221 taccgttaca ttcag
//
LOCUS       DAOQGJ010000531         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32107_length_2234_cov_3.293713, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000531 DAOQGJ010000000
VERSION     DAOQGJ010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32107_length_2234_cov_3.293713"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PK683_09215"
     CDS             <1..1512
                     /locus_tag="PK683_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNH08669.1"
                     /translation="GVAYITTVSSVWWTNPVVLNSGSISTASQIHLFNTDPGTTLPFN
                     AHKAAIVFKTSGPVVLGSSGCATNPAIGTNTKNFLWIEGNYISPSGLNTSGLAVTGTF
                     NVIKNFRIQNFNSGSCSSPGILLTGQNNFFEDVVIGNGASTGANGITVSAASYNFFKN
                     VTIFNMTNAGILLSGSSQNNTFQNITVMNTAGYGFLTSGTALNTSLMNVSVINGNNDG
                     IRIGGANNSLLMNALTVNNAGATNGGLVLNSTGLTVQNAGSSGNGTSNIVLTGASAYI
                     TGVLKGGGCSNIGSTGITASCAVQGTSDFNFTTGLTGIAFTVNSGLIFLDKVTSDDSK
                     NQSDATGSSSYGTITDWTHFDSFYRGWGLDGSFPSTGVRGAGSGVSVMRIYDWSLKSS
                     DSILRNVTPCPTDISDLNGADTNALTHSFSTGTVTMLRNAVEIVGDGIGDDDGFCEND
                     EACIYTPNIGVYQGHGNLILSSQASSTTKTCPDPGSADTVKNVTLYKYENNGY"
     gene            1882..>2234
                     /locus_tag="PK683_09220"
     CDS             1882..>2234
                     /locus_tag="PK683_09220"
                     /inference="COORDINATES: protein motif:HMM:NF027223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08670.1"
                     /translation="MLSGWRRKVIQSLCSDNRLDPSMTVVAPEPESGNWSDINILEPN
                     TKLTQVLNKQIPWELQYLNLCDITAFWFPSYWKHEQADVFPANIGPTSRFEFGFFLQE
                     YLKNPLKRKFIIGSP"
BASE COUNT          652 a          466 c          503 g          613 t
ORIGIN      
        1 ggggtcgctt atattacaac tgtttcttct gtctggtgga caaatcctgt tgtgctgaat
       61 agcggctcga tcagcacagc ctcgcagatt catctgttca atacagatcc gggcacaact
      121 cttcctttca atgcacataa agccgctatc gtatttaaaa cctccggtcc agtcgttctc
      181 ggctcttcag gatgtgcaac caatcctgca ataggaacaa atacaaaaaa ttttctttgg
      241 atagaaggta attatatcag cccaagcggt ttaaatacca gcggccttgc cgttacaggt
      301 acgttcaatg taattaaaaa cttcagaatt cagaatttta attcaggatc ctgcagcagc
      361 ccgggaatct tgctgacggg tcagaataat ttttttgaag atgttgtcat cggaaacgga
      421 gcaagcacag gagcaaatgg aatcacagtc agcgcagcat cttataattt ctttaagaat
      481 gtcacaattt tcaatatgac caatgcggga atactgcttt caggcagttc ccagaacaat
      541 acatttcaga atatcactgt aatgaatacc gcaggatacg gctttcttac atccggaaca
      601 gctttgaata cttcactgat gaacgtgtct gtcatcaacg gaaataatga cgggattcgt
      661 atcggcggag caaataactc actgctgatg aatgcactga ctgtaaacaa tgcaggtgca
      721 acaaacgggg gacttgtttt aaattctacc ggactcacag tacagaacgc aggttcaagc
      781 gggaacggta cgagcaatat cgttctcacc ggtgcaagtg cttatatcac cggtgtgtta
      841 aaaggagggg gctgttccaa cattggatct accgggataa cggcttcctg cgcagttcag
      901 ggaacatctg attttaattt cactacaggt ctgactggga ttgcttttac tgtaaactca
      961 ggattgattt ttctggacaa ggtgacatct gatgacagca aaaatcagtc tgatgcaaca
     1021 ggaagcagca gttacgggac aattacggat tggactcatt ttgacagttt ttatcgaggc
     1081 tggggactcg atggaagttt tccgagtaca ggagtacggg gagccggaag cggggtaagt
     1141 gtgatgagga tttatgactg gagcctgaaa tcctccgatt caattcttcg aaacgttaca
     1201 ccttgcccga ctgatatcag tgatctcaac ggggcggata ccaatgcact gacccacagt
     1261 ttttccacag gtacggtcac gatgctcaga aatgcggtgg agatcgtcgg tgacggaatc
     1321 ggggatgatg acggattctg cgaaaacgat gaggcctgca tttatactcc gaatatcggt
     1381 gtttatcagg gacacggaaa tctgattctc tcctctcagg catcttccac aacgaaaacc
     1441 tgtcctgacc ccggatcagc cgatacagta aagaatgtta cattatataa atatgaaaat
     1501 aacggatact gagatatata ttaataccgt tcttgtaact actcagccta gcggcttcgt
     1561 agttactgtt ttacgggggc ctgctggcgg tttcaagggt ttcgcccttg aaaatcccat
     1621 caaaattcca aatgaaaaca tacgctgagt agttacctgt tcttccatac aaaacttggc
     1681 ctttcgaact ttgattggat cggcagttta gaaaagcagt tgtgatgatt tcaattggtt
     1741 gctttttatc atattggaaa aggaacaatc aaaatgacag ataatataga acagttaaga
     1801 gttttatact caagaaatca tacaacagta tttcccagac tgggagtctt tttggcagga
     1861 cctacgccgc ccaccgggga aatgctttca ggctggaggc gaaaagtaat acagagttta
     1921 tgcagcgata atcggttgga tccaagtatg acagtagttg ctcctgaacc ggaaagcgga
     1981 aactggtctg acattaatat tttggagccg aatacaaaac tgactcaagt tttaaacaaa
     2041 cagattcctt gggaactgca gtatcttaat ctatgcgata ttacagcttt ctggtttcca
     2101 tcctattgga aacacgaaca ggcggatgtt tttcctgcaa atatcggacc tacgagccga
     2161 tttgaattcg gcttttttct ccaggaatat cttaaaaatc ctcttaagcg aaaatttatt
     2221 attggatctc caga
//
LOCUS       DAOQGJ010000532         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32156_length_2232_cov_2.923748, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000532 DAOQGJ010000000
VERSION     DAOQGJ010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32156_length_2232_cov_2.923748"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..1290)
                     /locus_tag="PK683_09225"
     CDS             complement(337..1290)
                     /locus_tag="PK683_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNH08671.1"
                     /translation="MILRDKPTMQGRQLALMKEKEEVLVQRDDGPEETLFGRKSRWFE
                     VKYKGIQGYAFGGFLEFTGKQNSVTKKTDVQKELPKTITNSIGMEFVLIPAGEFLMGC
                     SDGDSECYENEKPQHKVKITKPFYMGKYEVTQGQWKKVMGKNPSRFKNCGADCPVETV
                     SWNDTQTFIEKLCSQERTFLEIITFQKCKYRLPTEAEWEYAARAGTNTIWYGNLDEIA
                     WYDKNSGNTTHPVGKKRPNAFGLYDMLGNVEEWVEDWFSEDYYKSSPEFDPKGKYYWT
                     RRGFRGGCWLYTARFVRSSSRNYSPPELRHFNVGFRLVLSP"
     gene            complement(1631..2158)
                     /locus_tag="PK683_09230"
     CDS             complement(1631..2158)
                     /locus_tag="PK683_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH08672.1"
                     /translation="MKLWLQQKKRFYFSAIYVILTISLIAEEIVTFRLNENLARPSDF
                     FFSVLEVLLGIFSIVGVILIFREIRENNQNIASAREAIDSLKTQNKRLSDFNKSFWNS
                     VLSQFRDWGLSEAEKEIAAMLLRGLSNQQIAAIRGKSLKTIENQTFSIYQKSGTTGKL
                     EFIAYFLNPLLPEED"
BASE COUNT          574 a          555 c          446 g          657 t
ORIGIN      
        1 ttatgcggta gctggtcatc cgctctcctc gtcgttagga gtggccgccc tttcgcctga
       61 agggcgaaac ctagcaggcc agctttttac ccgcccgtcg gctttgtaaa aagctgccgg
      121 ggtgaggtta gcctcaaaat ttttttctcc ggaattctca gtgctccgtt cgcaaaaaag
      181 ccggtcgttt ggtttgcagc agcttcctag tttgcggcag cttcctagtt tgcggcagct
      241 tcctagtttg cggcagcttc ctagtttgcg tatcgaacca ccgactaatc ctgagggagg
      301 agcgcgatac agaaccattc acagacactc tgaaccttaa ggagaaagaa ccaagcggaa
      361 gccaacattg aagtgcctga gctcaggggg cgaatagttg cgagacgaag aacggacaaa
      421 cctcgcggta tagagccaac acccgccacg gaacccgcga cgcgtccagt aatatttacc
      481 ttttggatcg aactcaggtg aacttttgta atagtcttca ctaaaccagt cttccaccca
      541 ctcttctaca ttgcccagca tatcgtaaag cccaaatgca ttcggtcttt tctttcccac
      601 tggatgcgtt gtgtttccgc tgtttttatc ataccaggca atctcatcta aattcccata
      661 ccatatcgtg tttgtacctg cacgagcagc gtattcccac tccgcttccg taggaagtcg
      721 gtatttgcat ttctggaatg taatgatctc gagaaaagtt ctttcctggg aacagagttt
      781 ttcgataaat gtctgtgtat cattccagct cacagtttcc actggacaat ctgctccaca
      841 gtttttaaaa cgacttggat tttttcccat cactttcttc cactgtccct gagtcacttc
      901 atacttgccc atataaaacg gttttgtgat ctttaccttg tgttgcggtt tttcattttc
      961 ataacactca gagtctccgt cgctgcaacc catcagaaac tcccctgcgg gaatcagcac
     1021 gaattccatt ccgatggaat ttgtgatcgt tttgggaagc tctttttgaa catccgtttt
     1081 ttttgttact gaattctgct tcccggtgaa ttccagaaac ccaccgaatg cataaccctg
     1141 aattcccttg tatttcactt caaaccatct ggactttctt ccgaagagag tttcttccgg
     1201 cccgtcatct ctctgtacaa ggacttcttc cttttctttc attagtgcaa gctgcctgcc
     1261 ctgcattgtc ggtttatcac ggagaatcaa tccctcaggt tcaatgactt ccttgcggag
     1321 aagaacagaa cagcagtaaa gacaggagga gcaggattgt atttttcata taattcctta
     1381 gtattttgat atattgttta atgatggctt ttcagatcag attctttttg gaaaagaggc
     1441 ccctctaagg gcacacagcg ccccgacaga actccgaagc tttcaaactg caaatcctgc
     1501 tcataacgga attttccgta tcctgaaact gtcattttgg acagatatca gacatccatt
     1561 tcgcagatag actttcagat ccggaaaaat tcgtttcaga agtgcgagat aatcaggtgt
     1621 cttaaatccc tcaatcttct tctggaagaa gcggattcag aaagtaagca ataaactcca
     1681 gtttgcccgt tgttccggat ttttgataaa tggaaaatgt ctgattctct attgtcttca
     1741 ggcttttgcc tcgaatcgca gcaatctgct gattggaaag cccccgcaga agcatcgctg
     1801 caatttcctt ctcagcctca ctcaaacccc aatccctgaa ctgagacagc acggaattcc
     1861 agaaactttt attaaaatct gaaaggcgct tattctgagt cttcagcgag tcaattgctt
     1921 ctctggccga agcaatattc tgattattct ctctgatttc ccggaagatc agaattacac
     1981 ctacaatgga aaatattccc agaagaactt ccagaacaga aaagaaaaaa tcggagggcc
     2041 ttgccagatt ttcattcaga cgaaaggtta caatctcctc cgcaatcagt gagattgtaa
     2101 gaattacgta aattgctgaa aaataaaatc tttttttctg ctgcagccac agtttcattc
     2161 aagttcctta gggagtttcc accagcctag taaagggatt ccgtaaatgc cggactcacc
     2221 tatagactgt at
//
LOCUS       DAOQGJ010000533         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32175_length_2231_cov_3.715074, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000533 DAOQGJ010000000
VERSION     DAOQGJ010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32175_length_2231_cov_3.715074"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PK683_09235"
     CDS             <1..571
                     /locus_tag="PK683_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08673.1"
                     /translation="EILHLKDHIPPGLDGIIVSCLAKRGNQMNFFSYWSPENKSVYYS
                     LSLKVFNLIFQHSKSEKILSYLEHLNDYFGFIPPYFCREYDDFQWVKIYGTLSWQFPF
                     ETDYPLQKKFEDSLNRLNPLKPILFDLSNFISIGGAYGKIFEQFFKKYIKTVLLLNET
                     AVPYLRIIGYAGEYFSDRRDAMKKIQSLF"
     gene            complement(679..>2231)
                     /locus_tag="PK683_09240"
     CDS             complement(679..>2231)
                     /locus_tag="PK683_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH08674.1"
                     /translation="NSPLKAGNKVPDLEGIQWIKGKGVSLGTGLTIVEIWATWCGPCL
                     QTIPHLTELQKLYKGRLTIAGLTTESMDEVVPFVKELGKKMDYNVGIAPQDLYGAYME
                     GIDGIPNAFIINEKKQLLWQGHPYFLDALLPLILGKGGSEKAVLDLSRKKQEVLALHS
                     EIQGKVSEHLDKKKKLKQLASEVLAVLPEDSEITGILSEYQMFVPGETLPSLDEIEWF
                     PECSGKFSHKYNLIHTWSAIAEAEWSISLPMLCELQKKYGDRIFISALSSQDFDYISQ
                     FVESLPPGIQYAYMTVPAFEEFNVQNEEYGITYLFDSERTLLWKGRAAETPGILAALL
                     EKKGTGSTLLRHHSSVEAFEAMNSELYSKNTVTERDFKKLISAADKVLKINPSDFSVL
                     ASLCIQAARFGKEKVSEICETFDTSSFNANQLRQLVSEAVISLDSQYIPYHYAVKCLD
                     QALQLSPDSAECILLYADVLSYMHLTDEALKMVERGQEAWPDNLDLFFKKKILENIKE
                     CKSAYKVK"
BASE COUNT          603 a          483 c          446 g          699 t
ORIGIN      
        1 ggaaatactg catttgaagg atcatattcc tcctggactg gatgggatca ttgtttcctg
       61 cctcgcaaaa agaggcaatc aaatgaattt cttcagttac tggtcgcctg aaaataaatc
      121 tgtttattat tctctttctc tgaaagtgtt taatctgatt ttccagcatt ccaaatcgga
      181 aaaaattctt tcctatctgg agcatctgaa tgattatttc ggttttattc ctccgtactt
      241 ctgcagggag tatgatgatt ttcaatgggt aaaaatctac ggcacgctca gctggcagtt
      301 tccatttgaa accgattatc ctctgcagaa aaaatttgaa gattcactga acagactgaa
      361 tccattgaaa ccgattctgt ttgatttgag caattttatc agcatcggag gggcgtatgg
      421 aaaaatattt gagcagtttt ttaaaaaata cataaagaca gtactgctgc tcaatgaaac
      481 agctgtgcct tatctgagaa tcatcggtta tgccggggaa tatttttcgg acagaagaga
      541 tgctatgaag aaaattcagt ctcttttctg aatgatgacc gaacagatgc ttccgcctga
      601 ttttttataa ttacattgta tatcaagcgg aactgccgtt ccggcagtca gaattcccag
      661 tttctgagga aacgaacctc actttacttt ataagcagac ttacattctt tgatgttttc
      721 cagaattttt ttcttaaaaa agagatccag attgtccggc caggcttcct gtcctctttc
      781 aaccattttc agggcttcat cagtcagatg catataggaa agaacatctg catagagcaa
      841 tatgcattct gctgaatcag gggaaagctg cagtgcctgg tccagacatt ttactgcata
      901 atgatacggt atatactgag aatccagtga aatgactgct tcactcacta gctgtctgag
      961 ctgatttgca ttgaatgagg atgtatcgaa tgtctcgcag atttcagaga ctttttcttt
     1021 tccaaatctt gccgcctgaa tacagagtga ggccagtaca ctgaaatccg acgggttgat
     1081 cttcagcact ttatctgcag ctgaaatcag ctttttaaaa tctctttcgg tcacagtatt
     1141 cttagaatac agttctgaat tcatcgcttc aaatgcttcc acactggaat ggtggcgcag
     1201 aagagtgctg ccagtgcctt ttttttccag aagagcggca agtattcccg gggtttctgc
     1261 tgccctccct ttccagagaa gtgttctctc tgaatcaaag aggtatgtga tcccgtattc
     1321 ctcattctga acgttgaact cttcaaatgc gggaacagtc atatatgcat actgaatccc
     1381 aggaggcagg ctctccacaa actggctgat ataatcgaaa tcctgagagg agagagctga
     1441 aatgaaaatc ctgtccccgt attttttctg cagttcacac agcataggaa gagatataga
     1501 ccattccgct tcagctatgg ctgaccatgt atgaatcaga ttatatttgt gactaaattt
     1561 tcctgagcat tcgggaaacc attcaatctc atccagagaa ggcagagttt ctcccggaac
     1621 aaacatctga tattcagaaa gtattcctgt aatttcgctg tcttcaggaa gaactgccag
     1681 cacttcagat gccagctgtt tcagtttttt ctttttgtcg agatgttcag aaactttccc
     1741 ttggatctct gaatgcagtg ccagcacttc ctgttttttt ctggaaagat ccagaactgc
     1801 tttttctgat ccgcctttcc ctagaatcaa cggaaggaga gcgtccagaa aataaggatg
     1861 accctgccag aggagctgct ttttttcatt aatgataaat gcattcggaa ttccatcaat
     1921 accttccata taggctccgt acagatcctg aggagcaatt cctacattgt agtccatttt
     1981 tttcccgagt tcttttacaa aggggactac ctcatccata gattcggttg taagtcctgc
     2041 aattgtcagt cttcctttat ataatttctg cagttcagtc agatgaggga tcgtctgaag
     2101 acagggaccg caccaagttg cccatatttc cacaatggtc agtcctgttc ctaggctgac
     2161 tccttttccc ttgatccact gtattccttc gagatcagga accttgttcc ctgcttttaa
     2221 cggagaattt t
//
LOCUS       DAOQGJ010000534         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32207_length_2230_cov_2.747586, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000534 DAOQGJ010000000
VERSION     DAOQGJ010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32207_length_2230_cov_2.747586"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK683_09245"
     CDS             complement(<1..369)
                     /locus_tag="PK683_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNH08675.1"
                     /translation="MRPEDFPSYRLSVAPMMSYTDRHFRYILRKLTKYTLLYTEMIPM
                     DTVLRGDRKRFLEFSLEEKPLSIQFGGNDPDKLADCAAIAEDFGYDEVNLNVGCPSDR
                     VQNGNFGACLMLKPELVAGIL"
     gene            529..>2230
                     /gene="ptsP"
                     /locus_tag="PK683_09250"
     CDS             529..>2230
                     /gene="ptsP"
                     /locus_tag="PK683_09250"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNH08676.1"
                     /translation="MGKQNCKFQGIPASPGKFLGPCLKIISSDHVILESNIQRHETSD
                     EIKKFHTAAKKTKQELESIISKNSLDINENLQAILEAQIFMVEDPVLLESVKYRIEKH
                     LENAATALFHSIEKISKDFESISDQYFQERAEDIRDIGKRIEKNLLGTRSDEKILSSL
                     NKPVILVSKELTPSQILNINKFYIKGIATEKGGRTGHMAILARHFGIPAVLGLKNVHE
                     TVEKDGLGYIDGDNGIFVYNPAEIQKKLFSFEPIDRTLESKAKLSKTTLDSERITILA
                     NAESQDDIIEIKDRGFDGIGLYRSEMILLGRKGFPSEEDQYIQYAKMTKSLNGKTFVL
                     RTFDIGGDKSGEESEEIEENPFLGNRGIRYFLRNKDLFRQQIRAILRASQFGPISILL
                     PMISYLNELRQAKTLIRECERDLHKKGIETGNIKIGPMIETPACVFMLDALAKESDFF
                     CVGSNDLLQYIVAVDRNSSVLSDLYNPFHFGFLKSLEKIARFGRENGFPVSICGEIAS
                     DTNFTMLLIGMGYRSLSISVPTARKVAKIVSGSSVDHCLKLYNKIIELSAKEKYQELE
                     A"
BASE COUNT          724 a          431 c          476 g          599 t
ORIGIN      
        1 cagaattcca gcaaccagtt ccggtttaag catcaggcag gctccaaaat tgccgttctg
       61 aaccctgtca ctgggacagc cgacattcag attgacctca tcataaccga aatcttctgc
      121 tatggcagca cagtctgcca gtttatcggg gtcatttcct ccgaactgaa ttgaaagagg
      181 cttttcttcc agggaaaatt caagaaatct ttttctgtca ccgcgcagaa cagtatccat
      241 tggaatcatt tctgtatata atagagtata cttagtaagt ttccgaagaa tatatctgaa
      301 atgcctgtca gtatagctca tcataggagc cacactcagt ctgtatgagg gaaagtcctc
      361 aggtctcatt tcaatctgct ccatataacg attgaaactc tcagaatatc aggagtcaag
      421 agattcacag tctgacattt cttatttggg tatgatcgaa gaaaaagatt cggtatgatt
      481 aaagtcacta tttaattctt ggggttattt cgtcgaaatt gaattaggat gggaaaacag
      541 aattgtaaat ttcagggaat accggcttca cccggaaaat tcttaggccc atgccttaaa
      601 atcatatcca gcgatcatgt cattctggaa agcaatattc agagacatga gacctcagat
      661 gaaattaaaa aatttcatac tgccgcaaag aaaacaaagc aggaacttga aagcattatc
      721 agcaaaaatt cgctcgatat caatgagaac ctgcaggcga ttctggaagc tcagatattt
      781 atggtggaag atcctgttct tctggaatcg gtaaagtaca gaattgaaaa acaccttgag
      841 aatgcagcca cagctctttt ccattccatc gaaaaaattt cgaaggattt tgagtccatt
      901 tctgatcagt attttcagga aagagctgaa gacatcagag atattgggaa aagaatagaa
      961 aagaatcttc ttggaacaag aagcgatgag aagattctct cctctctgaa taaacctgta
     1021 atccttgtat ccaaggagct gactccttcg cagattctga atattaacaa gttttatatt
     1081 aaaggcattg ccacagaaaa agggggcaga actggccata tggcgatcct ggcacgtcat
     1141 ttcggcatcc ctgccgttct tggactgaaa aatgttcatg aaacggtgga gaaggacgga
     1201 ttaggatata ttgacggaga caatggaatc ttcgtctaca acccggctga aatacagaaa
     1261 aagctgttct cgtttgaacc gatagacagg actcttgaat ctaaggctaa actcagtaag
     1321 acgacccttg actcagagag gataaccatt ctggcaaatg cagaaagtca ggatgatatt
     1381 attgaaatta aggacagagg atttgacgga ataggacttt acagatcgga aatgatcctg
     1441 ctcggaagaa aaggatttcc ttcagaggaa gatcagtata ttcaatacgc aaagatgacc
     1501 aaatccctga acggaaaaac atttgttctg agaacatttg atattggagg agataaatca
     1561 ggagaggaat cagaagaaat tgaagaaaat ccttttttag ggaatagagg gatacgttat
     1621 ttcctgagaa acaaagatct ttttcgacag cagatcaggg caatcctcag agcaagccag
     1681 tttggaccaa tttccatcct gctcccaatg atttcctatc tgaatgaact gagacaggca
     1741 aaaacattga ttcgagaatg cgaaagagat ctgcacaaaa agggaataga aacaggaaac
     1801 atcaaaattg gacctatgat tgaaactccc gcctgtgttt ttatgctcga tgcgcttgca
     1861 aaggaatctg attttttctg tgtgggaagc aatgacctgc tgcagtacat tgttgcggta
     1921 gacagaaaca gttctgtcct ctccgattta tacaaccctt tccattttgg gttcctgaaa
     1981 agtcttgaaa aaattgcacg attcggcaga gagaatggtt ttcctgtcag tatctgcggt
     2041 gaaattgcct ccgatacaaa ttttacaatg cttttgattg gaatgggata caggtctctt
     2101 tccatttcgg tccctacggc aagaaaggtg gcaaaaatcg tttccggttc ttccgttgat
     2161 cattgcctga aactttataa taagatcatt gaactgtcag caaaggagaa atatcaggaa
     2221 ctcgaagccc
//
LOCUS       DAOQGJ010000535         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32275_length_2227_cov_3.186924, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000535 DAOQGJ010000000
VERSION     DAOQGJ010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32275_length_2227_cov_3.186924"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1643
                     /locus_tag="PK683_09255"
     CDS             <1..1643
                     /locus_tag="PK683_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08677.1"
                     /translation="ISSLNNAVDSSKGLLLLAAKADSRADYQKNLDLAKEEDKKIAGA
                     LTKIKKFLTERSAKYEEFVKESHGVLKLDDNPAKRFEEIEKLVLNLGETTSYVEKLQE
                     EYYRYDTQEYRKFAIPKELELHFKDKNRTYITLISKFKEFNDISTKGFEISKRAMQSS
                     TTGVKTVLWGGYAISLIIVVFVTYMMIRRIALPVEMAEQVANKFAEGDLTINLENTSQ
                     DEIGRLIRALGKASENLKRIIKEIGNTADHLASSAEELSATSRNLADGATNQASSLEQ
                     TASAVTEISESISYVSDSAKDQEKEVIETNKSMNELSKSIIQITETARSVNEGSQSAL
                     AEAKEGQLKVNETVKRMAAIEESSEQISEIINVINDISEQTNLLALNAAIEAARAGES
                     GRGFAVVAEEISKLAARSQKATREIADLITDSINKVNDGKAIVGQVVESLNKILNKSI
                     EAAQLSDAISNATKQQSSGNQKVLKSVSTLAQMAQSISRATNEMKISSKEISNAIEQV
                     NSVAQNTASSSEEMAASTSELATQSEKLTSLISIFKTS"
     gene            1644..>2227
                     /gene="cysE"
                     /locus_tag="PK683_09260"
     CDS             1644..>2227
                     /gene="cysE"
                     /locus_tag="PK683_09260"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNH08678.1"
                     /translation="MFENIKFIREHDPAAKSYLEVILCYPGLHAVWFHKIARRLYTWK
                     IPLIPRLINYWSRWITGIDIHPGAKIANGILIDHGTGVVIGETAEVGKGCLIYQGVTL
                     GGTGKESGKRHPTLLENVVIGAGAKVLGNITIQNNVRIGAGSVVMRDVPANCTVVGIP
                     GRVVKNNTNKAMTDDTLLDHSELPDPVAKVFSILV"
BASE COUNT          726 a          501 c          519 g          481 t
ORIGIN      
        1 atatcagttc cctcaacaat gccgttgatt cctccaaggg gctcctgctt cttgctgcaa
       61 aggcggactc cagagctgac taccagaaga atctggatct ggccaaggaa gaagacaaga
      121 aaattgcagg ggcactgaca aaaattaaaa aatttctgac tgaacgttct gcaaaatatg
      181 aagaattcgt aaaagaatcc catggtgttc taaaactgga tgacaacccg gcaaaaagat
      241 ttgaagaaat cgaaaaactg gtactgaatc tgggagaaac aacttcctat gtagaaaaac
      301 ttcaggaaga gtattacaga tatgacactc aggaatacag aaaattcgcc attcctaaag
      361 aactggaact ccatttcaaa gacaaaaaca gaacttacat cacactgatc tcaaaattca
      421 aagaattcaa tgatatctcc accaagggat ttgaaatctc caaaagagca atgcagtctt
      481 ccacaacggg agtcaagaca gtcctctggg gaggatatgc aatttcactg atcatcgttg
      541 tttttgtgac atatatgatg atccgcagaa tcgctctccc tgtagaaatg gctgaacagg
      601 tcgccaacaa atttgcagag ggcgatctga caatcaatct ggaaaacaca tctcaggatg
      661 aaatcggaag actcattcga gccttgggca aggccagcga gaatctgaaa cggatcatca
      721 aggaaatcgg aaatactgca gatcatctgg catcttctgc agaagaactc tctgcaactt
      781 ccagaaactt ggctgacggt gcgacaaatc aggcctcctc actggaacag actgcttctg
      841 cagtgactga aatctccgag tccatctcct atgtgtctga cagcgcgaag gatcaggaga
      901 aagaagtcat tgagacaaac aagtccatga acgaactctc caagtccatt atacagatta
      961 cagagactgc acgttcggtg aacgaaggat ctcagtccgc actcgcagag gcaaaagaag
     1021 gacagctcaa agtaaatgaa acagtcaaga gaatggctgc aattgaagag agctccgagc
     1081 agatttccga gatcatcaat gtcatcaacg atatatcaga acagacaaac cttctggctc
     1141 tgaatgcggc gattgaagcg gcaagggcag gtgagtcagg aagaggattc gcagtcgttg
     1201 cagaagaaat ttccaaactg gctgcaagat cgcagaaagc gacaagagag atcgcagatc
     1261 ttattacaga cagtatcaac aaggtaaatg acggaaaagc gattgtagga caggttgtgg
     1321 aatccctcaa caagattctg aacaagtcca tagaggcagc acagctctcc gatgcaattt
     1381 ccaatgcaac aaaacagcag agttccggaa atcaaaaagt gctcaagtcg gtctccactc
     1441 tggcgcagat ggcacagtcc atatccaggg ctacgaatga aatgaagatt tcctccaagg
     1501 agatctcaaa tgcaattgag caggtgaatt cggtggcaca gaataccgct tcttcgtctg
     1561 aagagatggc agcctccact tctgaactgg ctacacagtc ggaaaagctg acaagcctga
     1621 tctccatatt caagacatcg taagtgtttg agaatataaa attcatcaga gaacacgatc
     1681 cggcggcaaa gtcctacctg gaggtgattc tctgttatcc tggactccat gcagtctggt
     1741 ttcataagat tgcgcgcagg ctctatactt ggaaaattcc tctcataccc agactcatca
     1801 attactggag cagatggatc accggaatag acattcaccc aggtgcaaaa attgccaacg
     1861 gcatactgat cgatcatgga acaggtgtgg tcatcggtga gactgcagag gtaggaaaag
     1921 gctgcctgat ttatcaggga gtcactcttg gcgggaccgg gaaggaatcc ggcaaacggc
     1981 accctactct tctggaaaat gtagtcatcg gagcaggagc aaaggttctg gggaatatta
     2041 caatccagaa caatgtcagg atcggcgcag gttccgttgt gatgagagat gttccggcca
     2101 actgcactgt agtgggaatt ccaggcagag tcgtcaaaaa caacacaaac aaagcaatga
     2161 cagacgatac acttctcgat cactccgaac ttccggatcc ggtcgccaaa gtgttttcca
     2221 ttctggt
//
LOCUS       DAOQGJ010000536         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32276_length_2227_cov_3.181860, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000536 DAOQGJ010000000
VERSION     DAOQGJ010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32276_length_2227_cov_3.181860"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..791
                     /locus_tag="PK683_09265"
     CDS             183..791
                     /locus_tag="PK683_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNH08679.1"
                     /translation="MNYFSETETEPGMEMLEQIKNAPIQKKFLEERKIFLWGAVMDES
                     AKDIVAKLMYLEMTEPGKLITFYINSPGGVITSGMTIYDTMQMISSPVATVCMGLAAS
                     MGSLLLCAGEKGKRYIYPSGKVMIHQPSIGGQIVAPATDIRIHAQEIRKTKDKLNLIL
                     AEACNQPLEKIVQDTDRDYYMTAEEAIQYGIVDHLSSSIHVG"
     gene            793..1119
                     /locus_tag="PK683_09270"
     CDS             793..1119
                     /locus_tag="PK683_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08680.1"
                     /translation="MSEKISFEEAARMYLDFSKKEKEGEIFAILLDYLDAVYDKEEEP
                     ILLEDLTLFEIDDFLNFFLEDNFEDEAPELIKRSKSVLRSFEKFVKSKKLLSAEELEE
                     WTEVLK"
     gene            1098..2123
                     /locus_tag="PK683_09275"
     CDS             1098..2123
                     /locus_tag="PK683_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH08681.1"
                     /translation="MDRGLKVIQFPKAAYLQNSHSQTVFSSFFPPENRLTTDFFSEDI
                     FLETEDGTGDVLCLHHNPPAAFYKRLPVRFNGYYLVLIHGMEGTADSHYIVTMGELAL
                     RHGYGVIRVNLRGCGQGEGLSSRLYNSGTKDIDRVIRYIYYNINRNIILCGYSLSANM
                     VLKYMGEARRDEVRFFSAVSPPLDLKIGCDYIDSEKARFYRDFFLRSYREKIKKGLIK
                     APKAMLDAASKAETLFDFDDFLTAPLYNYKGALDYYKDCSSLPHIPKIRHQGILIHAD
                     DDPLIPDELFRGVKWRRYPNLTVILTKGGGHVGFLCKKTKEIPDGRWLNHTLLHYFAE
                     KTGVYEY"
BASE COUNT          674 a          436 c          514 g          603 t
ORIGIN      
        1 tgtgcattga atacagaata cagaaaaaac agtcaaaaca gaagctgatt tcatctattc
       61 acctcgcggt aagtataaca gttctgttct gcgtttttgt caactttaat tgagtctgaa
      121 gcacattttc ctgaaaagaa aaagattgat tttatagggc agaaaaagcg tactctaaaa
      181 ctatgaatta tttctctgaa acagaaactg aacccggtat ggaaatgctc gagcagatca
      241 aaaatgctcc aatccagaaa aaattcctgg aagaaagaaa aatctttctc tggggggctg
      301 tcatggatga atccgcaaag gacatcgtgg caaaactgat gtatctggag atgaccgaac
      361 cgggaaaact gattactttt tatatcaaca gtcccggcgg ggtgatcact tcaggaatga
      421 caatctacga tactatgcag atgatcagct cacctgtagc cactgtctgt atggggcttg
      481 ccgcatcgat gggatctctt ctcctctgtg caggggaaaa gggaaaacgt tatatctatc
      541 cgagcgggaa agtcatgatc caccagccga gcataggcgg tcagattgtt gcaccggcga
      601 ctgatatcag aattcatgcc caggaaataa gaaagacaaa ggataaactg aatttgattc
      661 ttgcggaagc ctgcaatcag cccctcgaga aaattgttca ggatacagac cgcgactatt
      721 atatgactgc agaggaagca attcagtacg gaattgtaga tcatctgtcc agttccattc
      781 acgtcggctg atttgtcaga gaagatttct tttgaagaag ctgcaaggat gtacctcgac
      841 ttttccaaga aggaaaagga gggggaaatc tttgcgattc ttctggacta tcttgatgca
      901 gtctacgaca aggaagaaga accgatcctt ctggaagatc tgactctttt tgaaattgat
      961 gattttctga atttttttct ggaagataat tttgaggatg aggctcccga actgatcaaa
     1021 agatccaagt cagtccttcg aagtttcgaa aagtttgtca aatcaaaaaa attgctcagt
     1081 gccgaggaat tagaggaatg gacagaggtc ttaaagtaat ccagttccca aaggctgcct
     1141 atctgcagaa cagccacagc cagactgtat tcagttcttt ttttcctcct gaaaacaggc
     1201 tgactactga ttttttcagt gaggatattt ttctcgaaac ggaagacgga acaggggatg
     1261 ttctctgtct tcaccacaat cctccagccg cattttacaa aagacttcca gtccgtttca
     1321 acggatacta ccttgtcctg attcacggga tggaaggaac tgcggacagt cattacatag
     1381 tgacaatggg ggagctggca ctcagacacg ggtacggtgt cattcgggtc aatcttcgag
     1441 ggtgcggaca gggagaagga ctgtcttcga ggctttacaa ttccgggaca aaggacatcg
     1501 acagagtcat aagatatata tattataata taaacagaaa tattatattg tgcggttatt
     1561 ctctgtccgc aaatatggtg ctgaagtata tgggagaggc aagacgggat gaggtgcgtt
     1621 ttttctctgc agtgtctccg cccctggatc tgaagatcgg atgtgattat attgattcgg
     1681 aaaaagctcg gttttacaga gatttttttc tccggtccta cagggagaaa ataaagaagg
     1741 gactcatcaa ggctcctaaa gcaatgctgg acgccgcatc gaaggctgag actctttttg
     1801 actttgacga ttttcttact gcacctcttt ataattacaa aggggctctg gactattaca
     1861 aggactgctc ctctcttcct catatcccaa agatccgtca tcagggaata ctgattcatg
     1921 cagacgacga tcctctgatt cctgatgaac ttttcagagg agtcaaatgg agaagatatc
     1981 ccaatctcac agtgattctt acaaagggag gcgggcatgt aggctttctc tgcaaaaaga
     2041 cgaaagagat tccagacgga agatggctga atcacacact tctgcattat tttgcggaaa
     2101 aaaccggcgt atacgaatat tgatctgtct caaaaaaaga gaaatcctgc ttgactgttt
     2161 tttggaaatg gtgtaggtag agtatatgct ggagaagttg tataattcat tgaatataat
     2221 aatttta
//
LOCUS       DAOQGJ010000537         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32376_length_2223_cov_3.570572, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000537 DAOQGJ010000000
VERSION     DAOQGJ010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32376_length_2223_cov_3.570572"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK683_09280"
     CDS             complement(<1..353)
                     /locus_tag="PK683_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH08682.1"
                     /translation="MRPFKVLGVQQIAIGGEDKSKLKKFWIDLMGLNITNTFKSEKEN
                     VDEDIAAIGKGPYAVEVDLMQPIDPSKSPKVHEPKLNHIGLWIDDLPACVQWLTEQKV
                     RFTPGGIRKGASGFDV"
     gene            complement(350..>2223)
                     /locus_tag="PK683_09285"
     CDS             complement(350..>2223)
                     /locus_tag="PK683_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNH08683.1"
                     /translation="AVSAAVAVVKNPEITVKELLKILPGPDFPTGGIIIGGDGLHSAY
                     TSGKGSIRIRSKVEIEQTSKGRDMIVITEIPYQVNKRTLLEKIGELVNEKTVEGISEI
                     LDLSDRKGIRIEIHIKKDYNSQVILNQLLKLTQLQVSYGITMLAILDGKPKIFNLKEM
                     LVAYVAHRNEVIVRRTKYELRKAEEKAHILEGLKIALDNIDEVIRIIRASKTPDEAKT
                     NLMSSFSLSEVQTKAILDMRLQRLTSLEVRKIMEELEEIRAVIADLKDILSKPERVSE
                     IVVQELEAVAQKFGTKRKTEISLESVDTTGFDAVDLIADEDVVVQITEDQYVKRLPID
                     TFKRQKRGGKGVQGGSTKRDDIIKMIRIASTHDTLMLFSDRGKAYALKVYELPQATKE
                     ARGKSLRAILNLQEGERITSICNVKDFSEGELVMVTKLGIIKKTKIAEFSNVKKNGII
                     AIGLRENDSLLFADAIDPKDDIILASRDGLAVRTNLSKLRSQGRTAGGVIGMRLSDDD
                     VLIGVAIPEKESDLLVITEKGYGKRTEYSEFAVKGRGGLGLTYLKITDKNGPAIGVAT
                     VRKNDEIIIMAQSGMIIRVNVEEISQVGRSTVGVRVVNIKDEDKVIDFALISEREEE"
BASE COUNT          473 a          576 c          444 g          730 t
ORIGIN      
        1 acatcaaaac cgcttgctcc ctttctgatt ccgcccggag tgaatctgac tttctgttct
       61 gtgagccact gcacgcaggc aggaagatcg tctatccaga gtccgatatg attcagctta
      121 ggttcatgaa ccttcgggct tttggaagga tcaatcggct gcatcagatc cacttccacc
      181 gcatacgggc ccttgcctat ggcggctata tcctcatcca cattctcttt ttcactcttg
      241 aatgtgttcg taatgttcag tcccatcaga tcaatccaga attttttcaa tttggattta
      301 tcctcgcctc cgattgctat ctgctgcact ccaagtactt taaacggtct cattcttcct
      361 ccctttctga aatcaatgca aaatcaatga ccttgtcttc atccttgatg ttgaccactc
      421 ttaccccgac tgtggatctt cctacctgag aaatttcttc cacattcaca cgtattatca
      481 ttcctgactg cgccataatg ataatttcat catttttcct tacagttgcc actccgattg
      541 caggtccgtt cttgtcagtg atcttcagat aagtgagtcc gagaccgccc cttcctttca
      601 ctgcaaattc agagtactcc gttcttttcc cgtagccttt ttcagtgatg accagaaggt
      661 cgctttcctt ttcgggaatc gccacgccga tgagaacatc atcatctgaa agtctcatcc
      721 cgatcactcc gcctgcagtt ctgccctgac ttctgagttt ggaaagattg gttctgactg
      781 caagtccgtc acggcttgca aggataatgt cgtctttcgg atcaattgca tctgcaaaca
      841 ggagagagtc gttctcacga agtccgatcg ctatgattcc gtttttcttc acattggaga
      901 attctgcaat cttggttttt ttgatgattc caagcttggt caccatgaca agctcacctt
      961 cagagaaatc cttgacattg cagatggaag tgattctttc tccttcctga agattcagta
     1021 ttgcacggag acttttccct ctcgcttcct ttgtggcctg aggaagctca tatactttca
     1081 gcgcatatgc cttgcctctg tcggaaaaga gcatcagggt atcgtgagtg gatgcgatac
     1141 ggatcatctt gatgatgtcg tctcttttag tcgatcctcc ctgaactcct tttccgcctc
     1201 ttttctgtct tttgaatgtg tctataggaa gacgcttcac atactggtct tcagtaatct
     1261 gaacgactac atcttcatct gcaatcagat ccactgcatc aaaccctgta gtgtccacgc
     1321 tttcaagact gatttccgtc tttctctttg tcccgaattt ctgtgcaact gcttccagtt
     1381 cctgaactac aatttcactg actctttccg gtttggaaag aatatccttc aggtctgcaa
     1441 tgactgccct gatttcttcg agttcttcca tgattttccg gacttcgaga gaggtcagtc
     1501 tctgcagtct catatcaagg attgcctttg tctgcacttc ggaaagactg aaactgctca
     1561 tcagatttgt cttggcctcg tcaggggtct tggaagcacg gatgatccgg atcacttcat
     1621 caatattgtc gagtgcgatt ttcagccctt ccaggatatg agctttttct tccgccttgc
     1681 ggagttcgta ttttgttctt ctgacaatga cttcatttct gtgcgccaca taggcgacga
     1741 gcatttcctt gagattgaat atcttcggct ttccgtcgag aatcgcaagc atcgtgattc
     1801 cgtagctcac ctgaagctga gtcagtttca ggagctgatt cagtataacc tgcgaattat
     1861 aatctttttt aatatgtatt tcaatacgga ttccctttct gtccgaaaga tccaggatct
     1921 cactgattcc ttctacagtc ttttcgttta caagttcacc gatcttttcg agcagtgtcc
     1981 tcttgttcac ctggtagggg atttctgtga tgacgatcat atcccttccc ttggaagtct
     2041 gttctatttc cactttggaa cggattctga tgcttccttt tccggaagta tacgcagaat
     2101 ggagtccgtc tcctccgatg atgatccctc cggtagggaa atccggaccg gggagaattt
     2161 tcaggagttc cttgactgtg atttcgggat ttttcaccac tgcgacggcc gcactgactg
     2221 cct
//
LOCUS       DAOQGJ010000538         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32389_length_2223_cov_2.549815, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000538 DAOQGJ010000000
VERSION     DAOQGJ010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32389_length_2223_cov_2.549815"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2223)
                     /locus_tag="PK683_09290"
     CDS             complement(<1..>2223)
                     /locus_tag="PK683_09290"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNH08684.1"
                     /translation="KILHDKRNLPAFRLTDIKIIIPDVPAYHLGIKNVLESLNLSFSF
                     TNNLFGKKLSPYYTAVSALLDLTDSEFLPEKALALFRNPCFAPSVSGSRIVPDIYAWQ
                     NILNSSRITGFLDEIERQEKGHRNDRRFTWEHLWDRLASQDIADSAETSDFLSDSEDF
                     IMITSSLFHDLISLKNSEGPEFFADLFERILEIYLDPEMPESETDSMQLARMNVTVHD
                     RIISSLSEVRKLQQFSDPEDRIPYEMYRQIISDTLDSMQEGSSQVYKSGITVGTVKDT
                     LDAEVSCLFCTGLDEDSFRSRADLDTYISVNPDNTEEEKDIHLTKLRYLSHCFSYGAE
                     ELYFSYTNYDTEKDRRRYPAPEYEIIRTKLSSIYGPNQFHEHKISLYPHEEYPASFPE
                     TLSEKEVFEMTVMKAWESETEFNTVSPSWEGRDLNLPEIFKDSEDTVRRMFSRNSGKK
                     ISRLGKRSAASPAEIANYIECPRKYVYTHIMNTASDYEPEEENYSLNPLIRMNILNSV
                     VQKFVKNPGLEIRNLQDDSVLFSELPMGLAGQAQKETLLEYISDEMLPFLVKNFSESR
                     VIFDLHSGAGDFQNEERSLPKARFLDLEISGRIPVAAAFQNTVRIGQAVYGKKIKTSQ
                     ILQSLIWHWTVQNSENIQKRMFEIFSLQKGIIEPFIIHFPIKNPPEIRLIETETVTPE
                     LLMPAVQNFVNNIFPIYPVHGDGSRDNICNYCPIVKTCFGYNYEFESIHPDIEESLNK
                     L"
BASE COUNT          605 a          447 c          468 g          703 t
ORIGIN      
        1 caatttattc agactttctt ctatatcagg atgaattgac tcaaattcat aattatatcc
       61 aaaacatgtt tttacaattg ggcagtaatt gcatatattg tctctggacc cgtcgccgtg
      121 taccggatag attgggaaaa tattattcac aaaattctga actgcgggca taagcagttc
      181 aggggtgact gtctctgttt caatcagacg tatctcagga gggtttttta taggaaaatg
      241 gattataaac ggctctatta ttcctttctg aagactgaag atttcaaaca tccgtttctg
      301 aatattttct gagttctgca ctgtccaatg ccagatcagc gactgaagta tctgagaggt
      361 ttttattttc tttccgtaca ctgcctgtcc tattctgacc gtattctgaa aggctgccgc
      421 aacgggaatc cttcctgaaa tttcaagatc cagaaaccgt gccttaggaa ggcttctttc
      481 ttcattctga aaatctcctg ctcctgaatg cagatcaaaa ataaccctgc tctcggagaa
      541 gttttttaca aggaaaggaa gcatttcatc tgaaatgtat tccaaaagcg tttccttctg
      601 tgcctgacct gcaagcccca ttggaagttc tgaaaaaagt actgagtcat cctgaagatt
      661 tctgatctcc agtcctggat tcttcacaaa tttctgaacg acgctgttca gtatgttcat
      721 tcggatgaga ggattcaagg aatagttttc ttcttcaggc tcataatcag aggcagtgtt
      781 cattatatgt gtatatacat attttcgcgg gcattcaata tagtttgcaa tttccgcggg
      841 agaggcggca ctgcgctttc ccagtctgga gatttttttc ccggaatttc tggagaacat
      901 tcttcttact gtgtcttccg agtccttgaa tatttccgga agattcagat ctcttccttc
      961 ccaggacggg gataccgtat tgaattcggt ttccgattcc caggctttca tcactgtcat
     1021 ttcaaaaacc tctttttcag agagggtttc aggaaaggat gcaggatatt cttcatgagg
     1081 atataatgat attttatgct catggaactg atttggtccg tatattgagc tcagttttgt
     1141 tcttatgatt tcgtattcag gagcaggata ccgtcttctg tccttttcag tgtcgtaatt
     1201 tgtatatgaa aaatacagtt cttctgcacc atagctgaag cagtgactga ggtatctcag
     1261 ttttgtaaga tgaatgtctt tttcttcttc tgtattgtcc gggttcactg aaatataagt
     1321 gtccaagtcc gcccttgacc gaaagctgtc ctcatccaga cccgtacaaa aaagacagga
     1381 aacttctgca tcaagagtat cctttacagt gcccacggtg atccctgatt tgtaaacctg
     1441 actgcttcct tcctgcatgg aatcgagagt gtcagatatg atctgtctgt acatttcgta
     1501 agggattctg tcttcaggat cggaaaactg ctggagtttc ctgacttcgg acagagaaga
     1561 aataattctg tcatgtacag tcacattcat tctggcaagc tgcattgaat ctgtttcgga
     1621 ttcaggcatt tccggatcaa gatatatctc aagaattctt tcaaaaagat cggcaaagaa
     1681 ttccggacct tctgaattct tcagagaaat cagatcatga aaaagagaag atgttatcat
     1741 tataaaatct tcagagtcag aaaggaagtc tgaagtttcc gctgaatcgg caatgtcctg
     1801 cgaagcaagg cggtcccaga gatgttccca agtgaagcgt cggtcgttcc gatgtccttt
     1861 ttcctgtctt tctatctcgt ccagaaagcc tgtgattcgg gaggaattca gaatattctg
     1921 ccaggcataa atatcgggaa caattctgct tcctgaaaca gagggagcaa agcagggatt
     1981 tctgaaaagt gcaagagcct tttcaggaag gaattccgaa tcagtcagat ccagaagagc
     2041 tgatactgca gtataataag gagacagttt ttttccgaaa aggttatttg tgaatgaaaa
     2101 actcaagttt aatgattcca gaacattttt tatcccaaga tgatatgcag gaacatcggg
     2161 gatgatgatc tttatgtccg tcagtctgaa ggcagggaga ttcctcttat cgtgaagtat
     2221 ttt
//
LOCUS       DAOQGJ010000539         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32392_length_2223_cov_2.274908, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000539 DAOQGJ010000000
VERSION     DAOQGJ010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32392_length_2223_cov_2.274908"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PK683_09295"
     CDS             complement(<1..675)
                     /locus_tag="PK683_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08685.1"
                     /translation="MSETENKETVQLQAGKKVPSLTGVKWVKGEEALLGEKLTVLIFF
                     NESAEYYSVISAMAEMAKKYKKKVSIAGLSSMNEENMNYFLSGTEETIQYGVGTVSEK
                     LYIAYMSGIEESAYAFVIDSEKHLLWSGDFQSMDTALAVVFKKKDEKNLLELGKKRQK
                     ILELNSEIHGNISKHPEEKKEIKTLISEYLEFFADDDDRIENLKALYSPWTPGETAEI
                     PEQIDWI"
     gene            1119..>2223
                     /locus_tag="PK683_09300"
     CDS             1119..>2223
                     /locus_tag="PK683_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08686.1"
                     /translation="MLNKIRQLKIITRLIITYILAGLILLGATAGMVYQTTIVKNIIE
                     QEIQQSHIEKTQFSEKTQVLGKQLISFLNKFMTVWIVFLFMIILCSFVMFYLIFQELV
                     IPLQQAFVVTDHMSKGSFDYELKVKTMDEMGELLQSIKIIRIIFRSVFSQILEVSNKF
                     FNSAKFLREFSDEFAEASEKIAANARISSSRLTELSDATSVLADSVSKETERIKEIRN
                     SIVLLNDSTVNIAHLFKELSAVSSRSAAQANSGKVKIEEVKVAMEEIGKNSKAIKDIM
                     GLINDISEQTNLLALNASIEAARAGAAGKGFSVVAAEVSRLADNTADRVKEIEKLIKT
                     TNQAVNNGTSKVDEAGRTMDAILESSSLVDKYID"
BASE COUNT          656 a          443 c          453 g          671 t
ORIGIN      
        1 aatccagtct atctgctcgg gaatttcagc agtctctccc ggtgtccagg gtgaatacag
       61 agctttcaga ttttctattc tgtcatcatc atctgcaaaa aactccagat attcagaaat
      121 cagtgttttg atttcctttt tttcctccgg atgcttcgag atatttccgt ggatttcaga
      181 attcagttcg agtattttct gtctcttttt gcccagctcc agaagatttt tttcatcctt
      241 ctttttgaaa accactgcaa gcgcagtatc cattgattga aaatccccgc tccatagcag
      301 atgtttttct gagtcaatga caaatgcata agcagattct tcaatgccgg acatataggc
      361 aatgtacaat ttttcagata cagtgccgac accgtactga attgtctctt ctgttcccga
      421 cagaaaatag ttcatattct cttcattcat tgaagaaagg cctgcaattg atactttctt
      481 tttgtacttt ttggccatct cggccattgc agaaattacg gaatagtatt ctgcactctc
      541 attgaaaaaa ataagaacag tgagtttttc tcccagaagg gcttcttcac ctttgaccca
      601 tttgactcct gtcagggaag gcactttttt accggcctga agctgtacgg tttccttgtt
      661 ttcggtttca ctcatgtcaa agcattaaat ttccggacag cagagagtca atcatttgga
      721 gaaatttttt atttctcagg aggggatttt ctccctgccc cgaagacggc aggaactgaa
      781 tatgttccag tcgtttctgc caggagcata ttccctgcat acagtcaata tttttactga
      841 attgtttcag gcattcccga ggagtcttca gattttgaaa tagactaaaa atcattatag
      901 aattgacgga aaatttattc tcttttctgc atcacttata ctgatcagta tacttttttc
      961 aatttaattg ccggaaggga aaaaaaggta tatctttttc cttttagctg atttactgtt
     1021 aaaagagacg gaaaggatgg cagcagtatg ccggacgaat caaatctcaa aatgaatgcc
     1081 tggatgatta aaaatctctt tttgaacaag gatttcttat gctgaacaaa attcgacagc
     1141 ttaaaattat cacaagatta attataacat atatattggc aggcctgatt cttcttggtg
     1201 caactgccgg gatggtctat cagacaacca tagtaaaaaa tatcattgag caggaaattc
     1261 agcagtctca catcgaaaaa actcagtttt cggaaaagac gcaggttctt ggaaaacagc
     1321 tgatcagttt tctgaataaa tttatgacag tctggatagt atttctcttt atgattatac
     1381 tgtgttcatt tgtaatgttc tatctgattt tccaggaact tgtcatcccg ctgcagcagg
     1441 catttgttgt gaccgatcat atgtccaagg gaagctttga ctacgaactg aaagtcaaga
     1501 caatggacga gatgggagaa cttcttcagt ccattaaaat tatcagaatt atattcagaa
     1561 gtgttttctc tcagatactt gaggtatcca ataaattttt caactctgcc aagtttttga
     1621 gggaattttc tgatgagttt gcagaggctt ccgagaaaat tgcagcgaat gcccgcatat
     1681 cctcaagcag actcactgaa ctttcggatg caacttccgt gcttgcagat tctgtttcca
     1741 aggagacaga gcgaataaag gaaatcagaa acagcattgt ccttctgaat gacagcactg
     1801 tcaacattgc tcatctgttc aaggagctga gtgcagtgtc aagccggtca gcggcccagg
     1861 caaattcagg aaaagtcaag atagaagaag tcaaggttgc aatggaagaa atcggaaaga
     1921 attccaaggc aatcaaggat attatgggac tcatcaatga tatctctgaa cagacgaatc
     1981 tgcttgctct gaacgcttcc atcgaggctg ccagagcagg tgctgcagga aaaggctttt
     2041 ccgtagtggc tgcagaagtc tcaagacttg cggacaatac tgcagacaga gtgaaggaaa
     2101 tagagaagct catcaaaact acaaatcagg cagtcaacaa cggaacttca aaagtggatg
     2161 aggccggaag aactatggat gcgattctgg aaagttccag tctggtggac aagtatattg
     2221 aca
//
LOCUS       DAOQGJ010000540         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32397_length_2222_cov_5.621597, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000540 DAOQGJ010000000
VERSION     DAOQGJ010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32397_length_2222_cov_5.621597"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2222)
                     /locus_tag="PK683_09305"
     CDS             complement(<1..>2222)
                     /locus_tag="PK683_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08687.1"
                     /translation="KELQPIPFKNAIDLSSNKKYLKYSVPPVLALLLMLFTAPSLIKK
                     PVNRLINNDKEFEREAPFTFQILNKNLKVAEFDNYTLEVKLEGNSIPDQIDVVYHDNP
                     YRLVKDSRGIFTYTFANVQDAFDFRLKAAGFNSKVYRLDVMKKAGMTDLNLKLVYPGY
                     IGRPEEYVKGSGDIIVPFGTKVYWDIRTRLADRVELEFEGNHAVSAQRAGDNRFTFLK
                     TAIQDQNYKIFTGNQALPVSDSMTYSIHVVMDENPVISLDQYVDSANQKLVYLSGNVS
                     DDYGFTKLQMVQQIKSEDGKVRTRTLPLQKPATKQGFYSYLFDIESLKLKPGDELTYF
                     FEVFDNDGAKGPKSARTSVMNFKKPSVKEMEKNIDKSTREIQSDLEKMVKESKKVNDA
                     INKLREKLLQQKSLNWQDKEQLNKLLEKQKELQDQLKETQKKLEEKRKAEEEIKQPDP
                     EMKEKQEKLDEMIKEMQNPEEQKLMDKIRELMDKLDKKESLKQLEQMSQQNEQQHKSL
                     DRLMELMKQLELEAKVQDQIDKLNELAEKEEKLAEQTENNQKDQEQLKKEQDQINKDF
                     QEVKKKMDEIQKENKELKAPKKLNDQKEEQQSTEQDLQDAKEQLEKKENKSASKSQKK
                     AGQKMKQMAQNMQNDMAASEKEQIELDMKAVRQLLENIVGLSFNQEDLIKQYNATMIN
                     TPRYIGLNREQKKIKSDFKMVDDSLQELSKRVMQIQSFIIKKVAEVNDNLDKTMDNLE
                     "
BASE COUNT          476 a          535 c          402 g          809 t
ORIGIN      
        1 cttcgagatt atccatagtc ttgtccaggt tgtcattgac ttcagcgact ttcttgatga
       61 taaacgactg tatctgcata acccgcttac taagttcctg cagactgtca tccaccatct
      121 tgaagtcaga cttgattttt ttctgttccc tgttcagacc gatgtatctc ggtgtgttga
      181 tcatggttgc attatactgt ttgataaggt cttcctgatt aaatgacaga ccaactatat
      241 tttccagcaa ctgacggacg gctttcatat ccagctctat ctgttccttt tcagatgccg
      301 ccatatcatt ctgcatattc tgagccatct gcttcatctt ctggccggct ttcttttggc
      361 tcttagaagc ggatttattt tcttttttct ccaattgttc cttggcatcc tgaaggtcct
      421 gctccgtact ttgctgctct tctttttgat cattcaactt cttaggcgcc ttcaattcct
      481 tgttttcttt ctgtatctca tccattttct tcttcacctc ttgaaagtcc ttattgatct
      541 ggtcttgttc cttcttaagt tgctcttgat ccttttgatt attctcagtc tgctcagcaa
      601 gcttttcttc cttttcggcc agttcattca gtttatctat ctgatcctgg accttagctt
      661 ccagttcaag ctgcttcatc aattccataa gccgatccag actcttatgc tgctgttcat
      721 tttgttgaga catttgctcc agttgtttga gggactcctt tttatccagt ttatccatca
      781 actcccggat cttatccatc aacttttgtt cttccgggtt ctgcatttcc ttgatcattt
      841 catcaagttt ctcctgtttt tccttcatct cagggtcagg ctgctttatc tcttcttctg
      901 ccttgcgttt ctcctccagt tttttctggg tctcctttag ttggtcttga agctcctttt
      961 gtttctccag caacttattc aactgttctt tatcctgcca attcaatgac ttttgctgca
     1021 gtaatttttc cctgagctta ttgattgcat cattgacttt tttcgattcc ttcaccattt
     1081 tttccagatc actttggatt tccctggtgg acttatcgat atttttctcc atctccttaa
     1141 cagaaggctt cttaaagttc atcactgaag tcctggcaga tttgggccct ttggcaccat
     1201 cattgtcgaa tacctcgaaa aaataagtta attcatcacc cggtttcaac tttaacgatt
     1261 ctatatcaaa aagatagcta tagaatcctt gtttagttgc gggtttctgt agtggcagtg
     1321 tcctggttct tacttttcca tcttcggatt tgatctgttg caccatttgc agctttgtaa
     1381 agccataatc atcagagaca ttgccactga gatagaccag tttctggttg gctgaatcca
     1441 catattgatc aagactgatt acagggtttt catccatcac gacatggatc gaatatgtca
     1501 tgctgtcact taccgggagt gcctgattgc ctgtgaagat cttataattt tgatcctgta
     1561 tggctgtctt caaaaatgta aatctattgt cgcccgcccg ttgtgcgctc acggcatgat
     1621 tgccttcaaa ttccaactca acccgatcgg caagtctggt cctgatatcc cagtatactt
     1681 ttgtgccgaa tgggacgatg atgtctccgg agcctttgac gtattcctca ggtcttccaa
     1741 tatagcccgg atagacgagt ttcaggttga ggtctgtcat tcccgctttt ttcattacat
     1801 ccaatcgata caccttgcta ttaaatccgg cagccttcag tctgaaatca aaagcatcct
     1861 ggacattagc aaaggtgtag gtgaaaatgc cccgactatc cttgaccaac ctgtatggat
     1921 tatcatgata gaccacatca atctgatccg gaatcgaatt cccctccaat ttgacttcca
     1981 gtgtatagtt atcaaactcg gcgaccttca ggtttttgtt cagtatctgg aaggtaaatg
     2041 gtgcctcccg ttcaaactcc ttatcgttgt tgatcaatcg attgacaggc ttcttaatca
     2101 gggagggggc ggtaaaaagc ataagaagca gcgccaatac cggcggcaca ctatacttga
     2161 gatatttttt attggagctc aggtcaatgg catttttaaa gggaattggc tgcaactctt
     2221 tt
//
LOCUS       DAOQGJ010000541         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32406_length_2222_cov_3.335948, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000541 DAOQGJ010000000
VERSION     DAOQGJ010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32406_length_2222_cov_3.335948"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK683_09310"
     CDS             complement(<1..445)
                     /locus_tag="PK683_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNH08688.1"
                     /translation="MIDAKNNKIRFNESASKWISSFDLSSIKCLIVCRGPVRKEAMDI
                     FDEIGIREYGILLSEKDSVVYPMALAPELRGFRFINNIHRVPDYMGAGKEEKDQRIID
                     ILNIAKSNGYTHIFAGYGFMAEDAEFIEAIEKSGITFMGPASSVAR"
     gene            complement(445..2088)
                     /locus_tag="PK683_09315"
     CDS             complement(445..2088)
                     /locus_tag="PK683_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNH08689.1"
                     /translation="MEDILSTLKNPLKSEEKTETKSSIYDNALKLGRDLQSKPQQGGG
                     TDRILVQHSKDRMTVWERIKVLTQSEPNIFYQNWGTNLDGASLVTGILNIGGRDVALY
                     GHDFTLRAGSMDATNGSKLARLIYMAGEHGIPLIGMNDSAGAFVPAGVGGLDGYSEAF
                     TALRKISGMVPSIMLMFGFNAGGGSYLPRQGSFMIQCENTFFGLTGPGVVKSVLGEDI
                     TAEDLGGPKVHAQSGVVDLVTPDELGSLKTALRLLSYLPDSNSSGAPFFQTSDPLDRY
                     TYEEDILFRKTFNAPTGMNTPFDITLYLQNICDHGAYMELQPLRARNIITAFGRLGGH
                     VVGFIANNSAVSSGQIDISAARKGSRFVRFCNLYNIPMIFLEDTTGFLPGKEQESGGI
                     VEEGRKLLDSIIDLRTPRITLIIRNAFGGAYATWNSYFTGADIVFTLPTARIAVMGPA
                     GKDYVYKDEMAKIAKDFKENSKNLGESEAKRIRDAALAALSHRYEEALMNPKEALALG
                     SVSSVILPGYTRKVLSENLNYLMRHYKPAPMSGVQREFE"
BASE COUNT          571 a          533 c          459 g          659 t
ORIGIN      
        1 gtcttgcaac actagaagca ggccccataa atgtgattcc cgacttttca attgcttcaa
       61 tgaattctgc atcctctgcc atgaatccat atcccgcaaa aatatgagtg tacccgtttg
      121 actttgcaat gttcaggatg tcaatgattc tctgatcctt ctcttccttg cctgctccca
      181 tataatcagg aactctgtgg atgttgttta tgaagcgaaa tccgcgaagt tcaggagcca
      241 aagccatcgg atagacgaca ctgtcttttt cggaaagaag gattccgtat tcacgtatgc
      301 ctatttcatc aaagatatcc attgcttcct ttctgacagg accgcggcag acgatcagac
      361 atttgatgct ggacagatca aaactggaaa tccacttgga tgcggattca ttaaaacgta
      421 ttttattatt ttttgcgtca atcattattc aaactccctc tgaactcctg acataggagc
      481 cggcttataa tgtctcatca gataattcag attttctgaa agaacctttc ttgtatatcc
      541 aggcaggatc acgctcgata cagaacccag tgccaaagct tccttaggat tcatcagtgc
      601 ttcttcgtat ctgtgtgaaa gggctgcaag agcagcatct ctgattcttt tggcttccga
      661 ttcccccaga ttcttggaat tttccttgaa gtcctttgca atttttgcca tctcatcctt
      721 gtaaacataa tcttttcctg caggccccat tacggcaatt ctggcagtag gaagagtgaa
      781 tacaatgtct gcgcctgtga aataagagtt ccatgtagcg tatgctccac cgaacgcatt
      841 ccgaatgatc agagtgattc taggggtacg aaggtcaatg atggaatcaa gaagctttct
      901 tccctcttcc acgataccgc ctgattcctg ctccttgcct ggaaggaatc ctgttgtgtc
      961 ttccaggaaa atcataggga tattatataa attgcagaat cttacaaaac gtgatccttt
     1021 tcttgcggca ctgatgtcta tctgtcctga ggacaccgca gagttgtttg caatgaatcc
     1081 gacgacatga ccgcccagtc ttccgaatgc agtgatgatg tttcttgcac ggagaggctg
     1141 aagctccata taggcaccgt gatcgcagat attctgaaga tacagtgtaa tgtcaaaagg
     1201 tgtattcatt cctgtggggg cattgaatgt ctttctgaaa agaatatctt cttcatatgt
     1261 atatctgtcg agcggatcgc ttgtctgaaa gaaaggagca ccgctggaat tgctgtcggg
     1321 gagataggac agaagacgaa gtgctgtttt gagagagccc agttcatctg gagtcaccaa
     1381 gtctacaacg ccgctctgcg catgaacctt agggcctcct aagtcttctg cagtaatgtc
     1441 ctctccaagc acggatttga ctacaccagg accggtaaga ccgaagaatg tgttttcaca
     1501 ctggatcata aatgatccct gtctgggcag ataagaaccg cctcctgcat tgaatccgaa
     1561 catcagcata atgcttggaa ccataccgct gattttacga agagcagtaa acgcctcgct
     1621 gtatccgtcc agaccgccca cacctgcagg cacaaaggct ccggcagaat cgttcatccc
     1681 gatcagagga attccgtgtt cccctgccat ataaatcagt ctggcaagtt tgcttccgtt
     1741 tgtggcatcc attgaaccgg cacgaagagt aaagtcatgt ccgtacagtg caacatctct
     1801 tccccctatg ttcagtattc ctgtcacaag tgaggcaccg tccagatttg ttccccagtt
     1861 ctgataaaaa atattcggtt cagactgagt caaaaccttg attctttccc atacagtcat
     1921 cctgtctttg gaatgctgca caagtattct gtcagttcct ccgccctgct gcggcttgga
     1981 ttgcagatcc cttccgagtt tcagtgcatt atcataaatg ctggacttgg tttctgtttt
     2041 ttcttctgat ttcaatggat tttttaacgt ggacaatata tcttccatac atccgctctt
     2101 cttttggatt tacaatagac agttttttct tatcccatat caaagaaaac atatttttag
     2161 agaatcgagt agacatttat catagcttga cctgaattct gcctcaagga ctcttctgcc
     2221 gg
//
LOCUS       DAOQGJ010000542         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32459_length_2220_cov_3.758430, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000542 DAOQGJ010000000
VERSION     DAOQGJ010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32459_length_2220_cov_3.758430"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="PK683_09320"
     CDS             <1..1737
                     /locus_tag="PK683_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08690.1"
                     /translation="LYRDRYVKKANSACEEFLSSPASDTDKSVALTVLGVILDEAGRY
                     TQAVERLRKAVDYDSKNIYAYYDLALAHKHAGQYTEARNVIMKAKSMAPNDSRISLLA
                     GNLLNETNDPKAAIEIYKEGISNSPDDPYLMYNLAISQYKQGQVADAVANFQRVIQVT
                     GRSQVSEFAHAYLGTIFYHRDDLNNAEHHFKEAAAIKPNEGKYLYNLGLILLKQKRTE
                     EAVSYLQKAIDSGSSDPAVFRFIAEAFEDLRMFDNATVALEKAMKIRPDDTDTMFQLA
                     DLYYSRGNLSSAEDLFRKIIKSTPGDTNTENALINLGIILDDMERYSEAVLAFEQAIQ
                     LNPKNDNAYYNLGIAYKNSGQPTKAIENWKKASALNPGESKNMEAIGDYYMEAGFHQE
                     AADEFNSIVKSNPGNYKARLKLADAYFRLKNYSNAEKSLFHILNNSKDGDEIKLAHRK
                     LALVYAEGDEKNKSKAKDEAYRGSHMDPDDMEGRLTLARILMDTNSMMDREKAIEELT
                     AVVRSDVKPKIAAQAYNYLGLCYYKNGEFKKAMREFQNSIDLDPSLTEAYSNKRAARA
                     AYESSIQGRAEL"
BASE COUNT          686 a          449 c          492 g          593 t
ORIGIN      
        1 ctttacagag acagatatgt aaaaaaggca aattccgcct gtgaagagtt tctcagctca
       61 ccggcttctg atacggacaa atctgtagcc ctcacagttc tcggtgtcat tcttgatgag
      121 gcaggcaggt atactcaggc ggtcgagaga ctgcgcaagg cagtggatta cgattctaag
      181 aacatttatg cctattacga tctggctttg gctcataagc atgcaggaca gtacactgag
      241 gcaagaaatg taattatgaa agccaagagt atggctccga atgattcaag aatatccctc
      301 ttggcaggaa atcttctgaa tgagacaaat gacccaaagg ctgcaatcga aatttacaaa
      361 gaagggattt ccaactctcc ggatgacccc tatctgatgt acaatcttgc catcagtcag
      421 tacaagcagg gacaggttgc tgatgcagtg gcaaattttc agagagtcat tcaggtgacc
      481 ggaagaagtc aggtctcaga atttgcacac gcttacttag gcacgatttt ttaccacagg
      541 gatgacctga acaatgctga gcatcatttt aaggaagccg ctgccatcaa gccgaatgag
      601 ggaaagtatc tctacaatct cggactgatt ctgctgaaac agaaaaggac tgaagaggct
      661 gtctcctacc tccaaaaagc aattgattca ggttcttccg atcctgcggt cttccgattc
      721 attgcagaag ctttcgaaga tctcagaatg tttgacaatg cgacagtggc actcgaaaag
      781 gcaatgaaga tcagaccgga tgatacagat acaatgttcc agctggcaga tctttattat
      841 tccagaggaa atctgtcttc tgcagaggat cttttcagaa aaattatcaa aagtactccc
      901 ggtgatacga acacagagaa tgcacttatc aatctgggta tcattctgga tgatatggaa
      961 agatattctg aagcagttct ggcatttgaa caggcaattc agctgaatcc taaaaatgat
     1021 aacgcatact acaatctggg tatagcatat aagaattcag gtcagccgac aaaggccatt
     1081 gaaaactgga aaaaagcttc tgcacttaat cccggcgaat ctaaaaatat ggaagcgatc
     1141 ggggactatt atatggaagc cggttttcat caggaagctg ctgacgagtt caattcaata
     1201 gtcaaatcaa atccaggaaa ttacaaggca agactcaagc ttgcagatgc ctatttccga
     1261 ctgaaaaatt acagcaatgc agaaaaatct ctttttcata ttctgaacaa ttccaaggac
     1321 ggcgacgaga ttaagctggc ccatcgaaaa ctggccctgg tctatgcgga aggggatgaa
     1381 aagaacaaat ccaaagcaaa ggatgaagcc tatcgcggat ctcatatgga tcctgatgat
     1441 atggaaggcc gtctgactct tgccagaatt ctaatggaca cgaattcaat gatggatagg
     1501 gaaaaagcaa ttgaagaact gaccgcagtt gtcagatccg atgtaaaacc gaaaattgcg
     1561 gctcaggcat acaattatct gggtctctgc tattacaaaa acggtgagtt taaaaaggca
     1621 atgagggaat ttcagaattc gatagatttg gatccttctc tgactgaagc ctacagcaat
     1681 aaaagagcag ctagagcagc ttatgaatct tctattcaag gaagagcaga gttgtaaatc
     1741 ctcaacaggg gttgtactgt tggatgtgca gcccctgttc catttttatc ctttctgctt
     1801 gcaggtgctt tcttataaac ccctgacaat cagcattacg atttccttgt actgctcatc
     1861 tgcgaactgc acttttttga gtgtttcaaa agagtattca agcgttctat cataaacctg
     1921 cctgtaaaaa acgtcaagaa ttgaatcttt tcttcggttc tttacacttt aggtgcaata
     1981 aaagcgggag gatgcaattt ttgtaaggct tcctcttatt cccgtttttc ccgtggttgt
     2041 ttattctttt tttaccgcga gggacacagg ggattgtctg tatggataag cctcttgtta
     2101 ttttcaggat aaaccgtttg taatctcgac aattgaagcc tgacggaacg atcttgtatt
     2161 gcagatcaac ttggggaatt tttccctaag gaacagtgaa aaattggaat catacagaaa
//
LOCUS       DAOQGJ010000543         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32463_length_2220_cov_3.575058, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000543 DAOQGJ010000000
VERSION     DAOQGJ010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32463_length_2220_cov_3.575058"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PK683_09325"
     CDS             <1..919
                     /locus_tag="PK683_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08691.1"
                     /translation="ESMRSWQKKYPPPVYSAVFYPFGGPDITNALVLFPDAEEYLLFG
                     LEPPGVIPDTESMQEESLTSGLNDLKLSVNTFFQVNFFITKKMEKSLGKKSFNTVTGL
                     IIFFLAMNGCEVTDVKKIAVGPLSTAVPSRDSDDSIGWKNPPHSRVPGVEISFRRNSG
                     RIQTVRYFMVNVSDEYLNRSSPNFLPYLKNGGRYATLIKSAAYLMHTDSSREPPPHFD
                     RIRRFILEQSDFIIQDDSGIPLRYFPRTEWKLGFHGKYESPISIFADRLQKNLRMEMQ
                     KSSTGKLPFSYSYTYQAGKSNLMTAERIK"
     gene            929..2059
                     /locus_tag="PK683_09330"
     CDS             929..2059
                     /locus_tag="PK683_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08692.1"
                     /translation="MKSLKYITVLYLFSMSVSACMSANPKVPDGPSPYSPIAKNIIVY
                     SIGGSVVSGIADSSALKTARLRLPFTAGGINLYQNGESLKWFTVQAVENSQKSSLSEQ
                     DKYLLVGLPELKSGELKFNYVMPEITWLPKLNASIIDSKTLTLQLQALITVGADEVYK
                     NCDVNLVLNNSLGVDRISGHTFSLNSFDLYPKRNIVYNLDNKTSQYTFLREWHTYLGK
                     DEVRVLVQVKNPFSIDLNQTGFSVESNQISIESGSIESQVRPGEILSLGAGTDDSIYT
                     FRSVKITESADKKTLPFNHKISYKITNKSNQEKKLRLVSSRVMGTDHRSVYHFKRQPD
                     ATPENTLIWILSLKPGVTETLEYDYDADIKDVPGENGFEAGG"
BASE COUNT          631 a          470 c          510 g          609 t
ORIGIN      
        1 ggagagtatg agatcctggc agaagaagta tccgcctcca gtctattcgg ctgtgtttta
       61 tcctttcggc ggcccggata ttacaaacgc gctcgttctt ttccctgatg cggaggaata
      121 tctgcttttc ggactggagc ctccgggagt cattccggat acggaatcga tgcaggaaga
      181 gagcctgact tccggactga atgatctgaa actgtcagtg aatacattct ttcaggtgaa
      241 ctttttcatc accaagaaaa tggaaaaaag tttgggaaaa aagtccttca atactgtaac
      301 aggactgatt atatttttcc ttgcaatgaa cgggtgcgag gtgacggatg tgaagaaaat
      361 tgctgtcggc cctctttcga ctgcggttcc gagcagggat tcggatgatt ccatcggctg
      421 gaaaaatcct ccgcattcca gagttcctgg cgtggaaatc agctttaggc ggaattccgg
      481 caggattcag acagtccgtt attttatggt gaatgtatcg gacgaatatc tgaacagaag
      541 ttctcccaat ttccttccgt atctgaagaa cggaggacgg tatgcaacgc ttattaaatc
      601 agctgcatat cttatgcata cagacagttc cagggaacct cctcctcatt ttgacaggat
      661 caggagattc attctcgaac agagtgactt tatcattcag gatgattccg gcattcctct
      721 ccggtatttt ccgcgcacag aatggaagct tggctttcac ggaaaatatg aatctccgat
      781 ctctattttt gcagaccggc tccagaaaaa tctgcgaatg gaaatgcaga aaagctcgac
      841 aggaaaactt ccgttcagtt acagttatac ttatcaggca gggaaatcga atctgatgac
      901 tgctgagcgc attaaataag gagaatccat gaagagctta aaatatatta cagtattgta
      961 tctgttttcg atgagtgttt ctgcctgtat gagcgcgaat ccaaaagtcc cggacggtcc
     1021 ctctccttat tctcctatcg cgaaaaacat cattgtctac agcatcgggg gaagtgttgt
     1081 ctccggtatt gcagattcct ctgctttgaa gacagcaagg ctccgtcttc cgttcactgc
     1141 cggcggaata aatctgtatc agaacggtga aagcctgaag tggtttacag ttcaggcggt
     1201 ggaaaattcc cagaaaagtt ccctttccga gcaggacaaa tatcttcttg tcggacttcc
     1261 tgaactgaaa tccggtgaat tgaaatttaa ttatgtgatg cctgaaatca cctggcttcc
     1321 gaaactgaat gcttcaatca ttgattcaaa aactctgact ctgcagctgc aggctctcat
     1381 tacagtcggt gctgacgagg tgtataaaaa ctgcgatgta aatcttgttc tgaacaattc
     1441 tctcggagta gacagaattt ccggtcatac attcagtctg aattcatttg atctttatcc
     1501 gaaaagaaat attgtatata atctggataa taagacttca caatatactt ttctcagaga
     1561 atggcataca tatctgggaa aggatgaggt gcgtgttctt gtgcaggtaa agaatccttt
     1621 ctccattgat ttgaatcaga ccggattttc ggtggaatcc aatcagatca gcattgaatc
     1681 gggatcgatt gaaagtcagg tcagaccggg agaaattctg tctctgggag caggaacgga
     1741 cgacagcatt tatacttttc gttccgtcaa aatcactgaa tcggcggata aaaaaactct
     1801 gccctttaat cataaaatat cttataaaat aacaaataaa tcgaatcagg aaaaaaagct
     1861 gcgtcttgta tcttcgaggg tcatgggcac cgaccacaga tcggtctatc atttcaaaag
     1921 acagccggat gcaactccgg aaaacactct gatctggatt ctctctctga aacccggggt
     1981 gactgaaaca ctggaatatg attatgatgc cgatataaag gatgttcccg gtgaaaacgg
     2041 atttgaagca gggggctgaa aactgcattg ggaatcctgt caaattaagg cagctttcat
     2101 agtagccgta agcgagcctg tcgaactatg accctcgtaa tagcatactt cgatgtgcaa
     2161 acgtggactc tgccgctcag gatgacagat cattgttaat acggcttgac agagtcgcat
//
LOCUS       DAOQGJ010000544         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32466_length_2220_cov_3.463279, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000544 DAOQGJ010000000
VERSION     DAOQGJ010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32466_length_2220_cov_3.463279"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PK683_09335"
     CDS             complement(<1..473)
                     /locus_tag="PK683_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HNH08693.1"
                     /translation="MIRKFILYLLITVGALASASHCGNSGSKKIPKAEKGVIDLRDWD
                     FEKDGVLKLDGEWEFYWNRFLKSGQFETADSRIFMDIPSVWNGKNFNNERLPYQGYAT
                     YRLRLLTREDQNDLSLKIMLINSAFKLYSSDVPSGSQGRPGKIAEETVPRMSPEIA"
     gene            complement(470..2185)
                     /gene="mnmG"
                     /locus_tag="PK683_09340"
                     /pseudo
     CDS             complement(470..2185)
                     /gene="mnmG"
                     /locus_tag="PK683_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
BASE COUNT          549 a          514 c          453 g          704 t
ORIGIN      
        1 gcaatttccg gactcattct gggaacagtc tcttccgcaa tctttccggg tctgccctga
       61 ctgccggaag gcacatcgga cgaatataac ttgaatgcag aattgatcag cataatcttc
      121 agtgaaagat cgttctgatc ctctctggtc agaagtctga gtctgtaagt ggcatatccc
      181 tggtaaggca gacgttcatt attgaagttt tttccgttcc agacagaagg aatgtccata
      241 aaaattctgc tgtccgccgt ctcaaactgc cctgatttca gaaatctgtt ccagtagaac
      301 tcccattctc cgtccagttt cagaacaccg tctttttcaa agtcccaatc cctgagatca
      361 atgacgcctt tttccgcttt cggaattttc tttgatcctg aatttccgca gtgagaagct
      421 gatgcaagtg ctccgacagt gataagtaaa tataaaataa attttcttat catttatttc
      481 ccatcagatg gtacatcaga atttcaatgt ctgaggggtc cactccggat attttggatg
      541 cactttccag attcgacggt ctgtgttttt ccagtttctg cagtgcttcc ttcttcattc
      601 cagggatatt ttcaaattta aagtctgcag gaatttcgag aagcaggtgc ttttctctcg
      661 attcaatgga ttccagttct cttttaatat aaccctcata cttgatttcc atctccagaa
      721 cctgaacaga ttcactgttt aaatttttca gctcaggcac aatggaactg cagtctgcaa
      781 gatttatctc cggtcttttg atgaatccag ccagacttcc tccgaatttg acagattcta
      841 ttttcctttt ctccagaatt aaattcagtt cttcagaagg tttcaaattc agtgaatata
      901 tcctgtttct gactgattct atttttgcgt acttttcttt cattctttca aattcagaat
      961 cggatacaag acccatttcg tgaccgtact tcatcagcct tctgtcagca ttgtcctgtc
     1021 tgagaagaag tctgtgctcg gcacggctgg tgaacattcg gtaaggatct tcaactccct
     1081 tatgaaccag atcatccaca agcacaccta tgtaactttc gcttctctga aagatcatca
     1141 taggcagatt cttaaatgac cggacagccc cgtatgcagc aacgagtccc tgtgcagccg
     1201 cttcctcata acccgttgtt ccgttgatct gcccggcatg aaaaagaccg cgaatttttt
     1261 ttgtctccag attctgcttc agttcagtag gatctacaaa atcgtactca actgcatatc
     1321 ctggacggat aatttctgcc ttttccagac ctttgataga gcggatgagc tgccactgta
     1381 cctgttcagg aagactggta gatgtaccgt tcacataaat ttctttagta tcgtaacctt
     1441 caggttccag aaaaatctga tgtctgtctc tctccgcaaa acgtacaatc ttatcttcaa
     1501 tggaagggca gtagcgggga ccgattgact ttatctgacc ggaatacata ggagaaaact
     1561 ccaggttgtc ctgaataatc tgatgagttc tctgattcgt atatgtaata tgacagggga
     1621 tctgtcttct tgtaattttt tccgtggagt aggaaaaggg ggagggatgt tcatcgccgt
     1681 cctgaatctg cagttcagaa aaatcaatcg tggatgcatg aagcctggga ggagttccag
     1741 tcttaagtcg acccagacga aatgaatttt ttcttaatgt atgagaaagt cccttagttg
     1801 ttccgtctcc aagacgtcct gcttctcttt gaagagttcc aatatgaatc atactggtga
     1861 gaaatgtccc tgtagtcaga atgacatgat ttgtgagtat ttcaaaacct cttcctgtga
     1921 cgactccttg aacccttccg tcttcgatat aaatgtcttc cactgtatcc tgacggatgg
     1981 aaagatgagg ggttttttcc agtgtgtgtt tgactgcaag ctggtattct tttttgtctg
     2041 cctgtgcacg gggagcccat acagagggac ccttggatgt gttcagcatt ttgaactgta
     2101 tgcctgtgga gtcaatgatt cttcccatca gacctccaag agcatcaact tcccttacca
     2161 tatggccttt ggcgatcccg ccgattggaa cactgcgatg tgtgggccgt tcccgtcaga
//
LOCUS       DAOQGJ010000545         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32520_length_2218_cov_2.956080, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000545 DAOQGJ010000000
VERSION     DAOQGJ010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32520_length_2218_cov_2.956080"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2218)
                     /locus_tag="PK683_09345"
     CDS             complement(<1..>2218)
                     /locus_tag="PK683_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH08694.1"
                     /translation="EGWQFPELAETGIEYKERRYSSPGFIETEWMQKTEAEIHGGEKL
                     RIQVAYRELPPLTEGNPLFLKEEQALLDAAGRRIADFISRKISVKILRENETLVTTMF
                     AQTTDSIVLVDAETGKFVDFNDSAHNDLGYTRDEFALLSVQDIQAEHSEEQIRENTER
                     TAKGNSTDFETRHRHKDGSVRDVSIRLRPIMIGGRQIISAVWRDITEQKIKEQEIEAY
                     KLHLEELVTSRTKELESINAEQNAVFESATIGIALIRERIVVRCNRKLEEILGYETGE
                     LAGKSTRHWYKSEEEFQKIGREVFRNLEEHGLYYRKEIQFCRKNGDTVWVKTKIKRLN
                     GNEPMSGVVAVFEDITDERESEEILKSAKEAAEKATQAKSFFLANMSHEIRTPMNAVI
                     GMTHLLNKTELTEKQRDYLNKIQKSGKHLLNLINDILDFSKIEAGKLSVEREQFNLEE
                     LFQNVSSFINEKADGKGLELIFDIDPKIPTHLIGDQFRIGQILLNYSSNAVKFTEKGE
                     ISIIVKIREKTADGLVLYFAVRDTGIGLTKDQQQLMFQSFQQADMSTTRKYGGTGLGL
                     IISKRLAELMGGDVGMESEHGKGSTFWFTVKVGIARDENASLTPDDDLKGLRVLVADD
                     SRLARDLLSRMLSGMTFSVKTVPSGESALEELKTAELEGTPYKIFFLDWLMPAMNGTE
                     TLQKMENLKLKNRPYIIMITAFGHEEISDRLKNIKVDEIITKPVTASLLFQASVRVL"
BASE COUNT          496 a          557 c          403 g          762 t
ORIGIN      
        1 cagcacacgg actgatgcct gaaaaagaag cgatgcagtc accggctttg taatgatttc
       61 atccactttt atatttttca gtctgtctga aatttcctca tgtccaaatg cagtaatcat
      121 tataatatat ggcctgttct tcaatttcag attttccatt ttctgaagtg tttctgtacc
      181 gttcattgcg ggcataagcc agtcgaggaa aaaaatcttg tacggtgttc cttccagttc
      241 tgcagtcttc agctcttcaa gtgcagattc ccctgaaggg acggtcttga ctgagaatgt
      301 cattcctgaa agcatcctgg aaagcagatc ccttgccaag cgactgtcgt ctgccacaag
      361 tactctgagt cccttcagat catcatcagg agtcaggctt gcattctcat ctctggctat
      421 cccgaccttt actgtaaacc agaatgtgct tccttttcca tgctcacttt ccattccgac
      481 atccccgccc ataagttcgg cgagtctctt ggatattatc agtccaagac ctgttccgcc
      541 gtattttctg gttgtggaca tatctgcctg ctgaaaactc tgaaacatca gctgctgctg
      601 atctttcgtg agtccaatcc ccgtatctct gactgcaaaa tagagcacaa gcccatctgc
      661 agttttttcc ctgattttta cgatgatgga aatttcacct ttctcagtga acttgactgc
      721 gttggaactg tagttgagaa ggatctggcc tattctgaac tgatctccta tcagatgcgt
      781 cggaattttc ggatctatgt cgaagataag ctcaagacct ttcccgtctg ctttttcatt
      841 tataaaactg gatacattct gaaacagttc ctccagattg aactgttctc tttccactga
      901 aagttttcct gcttcaatct ttgaaaaatc aagaatatca ttgatcagat tcagaagatg
      961 ttttcccgac ttttgtattt tgttcagata gtctctctgt ttttcagtca gctctgtctt
     1021 gttcagcagg tgagtcatcc ctatgactgc attcatagga gttctgatct catgactcat
     1081 attcgccaga aagaatgatt ttgcctgagt ggctttctct gcagcttctt ttgctgactt
     1141 cagtatttct tccgattccc tttcgtccgt aatgtcctca aatactgcca ccacaccgct
     1201 catcggttca tttccattca gtcttttgat ctttgttttt acccagaccg tatctccgtt
     1261 tttcctgcag aactgtattt ccttccgata gtaaagacca tgttcttcaa gattccgaaa
     1321 aacttccctt ccgatttttt gaaactcttc ttcactttta taccagtgac gggtcgactt
     1381 tcctgcaagt tcacctgtct cataacccaa tatttcttcg agctttctgt tgcatctgac
     1441 cacaattctt tcccttatca gtgcgattcc tattgtggcg gattcaaaaa cggcattctg
     1501 ttccgcattg atggactcaa gttcctttgt tctggaagtt acgagttcct caagatgaag
     1561 cttatatgct tctatctcct gttcttttat tttctgctct gtaatgtctc tccataccgc
     1621 cgaaataatc tgacgtcctc cgatcataat cggtctgagt cttatggata catctcttac
     1681 acttccgtcc ttatgtctgt gtctggtttc aaagtcggta gaatttcctt ttgccgtcct
     1741 ctctgtgttt tccctgatct gttcttcgga atgttctgcc tgtatgtcct gtacagaaag
     1801 taatgcaaat tcatctcttg tatatccaag atcattatgt gctgaatcat tgaaatctac
     1861 gaactttcct gtttctgcat ctacaagcac tatggaatct gtggtctgag caaacattgt
     1921 ggtgacaaga gtctcattct cccgcagtat cttgacagaa attttccggc tgatgaaatc
     1981 tgcaattctc cttccagcag catccagaag tgcctgctcc tccttcaaaa aaagaggatt
     2041 tccctcagta aggggaggca gctctctgta agccacctgt atcctgagtt tttcgccgcc
     2101 gtgaatctca gcttctgttt tctgcatcca ttccgtttca atgaagcctg gactgctgta
     2161 ccgcctttcc ttgtattcta tgccggtttc agcaagttcg gggaactgcc atccttcc
//
LOCUS       DAOQGJ010000546         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32575_length_2216_cov_2.892642, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000546 DAOQGJ010000000
VERSION     DAOQGJ010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32575_length_2216_cov_2.892642"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK683_09350"
     CDS             complement(<1..201)
                     /locus_tag="PK683_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08695.1"
                     /translation="MEFSAWGSEFRENGKQRLVEHYLPLLAVHYPLFFRLFPELQAPN
                     PELYFIHTDSFKQKGENYEKNSD"
     gene            complement(651..1724)
                     /locus_tag="PK683_09355"
     CDS             complement(651..1724)
                     /locus_tag="PK683_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNH08696.1"
                     /translation="MREPGGYLHTDHLGSVTMVTDGHGNVVSGGSMGGKANTVYKPYG
                     EIDRMNSSGPDITKHKYTGQIEDKESGLYYYKARYYDPAIGRFLTPDSIADGNQNFGM
                     NRYMYVNGSPVNFRDPSGNLCAGNMYGILAFRYFGAVGAAALGTYKNYTGGGLNCSGG
                     KLPYEHNALMFLTFAHIAGNNSSAEEQRLLMLGTELYYWHQRRNGDPTFLNMMSFRQR
                     DAFYLYQFSQAKNDIERALIYESYMLQSAKGRLVEPSDDFDRGSFLHDQYGSDPADRK
                     SRTANKKWMVNAWKTANGPLDYMAAATGTFLFSSLNLTNDALDFRQKQLNWLGERTGI
                     KMNGTFIKFTPPKCSGCSLKFYN"
BASE COUNT          590 a          563 c          434 g          629 t
ORIGIN      
        1 gtcagaattt ttttcatagt tttctccttt ttgtttaaat gaatcagtgt gaatgaagta
       61 gagttcaggg tttggagctt ggagttcggg gaacagccga aaaaaaagcg gatagtgaac
      121 agcgagcagt ggcaaatagt gttcaaccaa tcgctgcttg ccattttctc tgaactctga
      181 accccaagca ctgaactcca aactctaagt atatatgtta tcattacata gcccccttcc
      241 gaacccaaag ccctgatcca gaactccaat tatttttcat tacaaaatat cctccctatc
      301 cgccaatcgc tgctcgctct tttctccaaa cgccagactc tgaaccctga acccctatta
      361 attatcatta caaaacaccc tccctgttcc ctaccatgca ggcagataat ccggtttttt
      421 actgcattga cgcagcttct ggttgtgcac gaggcaatcg agaagaagaa cactgaaaaa
      481 ttgtgaatac tctggtgttc ccttttccat catatgatag tctaaaacac atgaactgct
      541 gtcacccaaa gtgtcatgtt cattctcaca attatcctgc tgagagcctt tcgtacaaga
      601 aaaggcaata accataaaca taaaaataat caatctattc attctttccc tcagttataa
      661 aatttcaaag agcaccctga acatttagga ggtgtaaact taataaaagt cccgttcatt
      721 tttatccctg ttctttctcc gagccagttc aactgtttct gtcgaaaatc cagagcatca
      781 tttgtcaggt tcagagagct gaacagaaat gttcctgtgg cggctgccat ataatccaaa
      841 ggtccgtttg cagtcttcca ggcattgacc atccatttct tattggctgt cctgcttttc
      901 ctgtctgcag gatcactccc atactggtcg tgcagaaaag atcccctgtc aaaatcatct
      961 gaaggctcaa caagtcgccc cttggcactt tgaagcatat atgattcata tattaaagct
     1021 ctttcaatat catttttagc ctgactgaac tgatagagat aaaatgcatc tctctgtcga
     1081 aaactcatca tattcagaaa tgtaggatct ccgtttcttc tctgatgcca gtaatacagc
     1141 tctgtcccaa gcatcaaaag tctctgttct tcagcagatg aattatttcc tgctatatgg
     1201 gcaaaggtca gaaacatcag tgcattgtgt tcatagggaa gttttccgcc gctgcagttc
     1261 agtccgccgc cggtataatt tttgtatgtt cctaatgcag ccgcaccgac agctccaaag
     1321 tatctgaatg ctaaaatacc atacatattt ccggcacaga gatttccgct tggatctctg
     1381 aaatttacag gacttccgtt gacatacata tacctgttca ttccgaaatt ctgattaccg
     1441 tctgcgatag aatccggtgt cagaaatctc ccgattgcag gatcatagta acgggcttta
     1501 taataataga gaccggattc cttgtcctct atctggcctg tgtatttatg ttttgtgatg
     1561 tccgggccgg aggagttcat cctgtcaatt tctccgtagg gtttgtaaac ggtattggct
     1621 tttccgccca tactgccacc gctgactaca ttgccatgac cgtctgtcac cattgtcacc
     1681 gatcccagat gatccgtgtg cagatatcca cccggctcgc gcaactcgcc cctagcaggc
     1741 ctgcgttttg cccgcctgaa gggctctgca aaactcgcct cattcacagg acgctcaata
     1801 cctgacccag aactgccgcc aggcacgtac ccagggtcac tgatggatgc agtattttca
     1861 ttcacaccat tctgcatcag tatccaaggc ggtgtgcctt gagctttctt cgaactgttt
     1921 cctctgcagg agagaagtat gaggaagatt aggaggagga aaaaagggat cagggcttgg
     1981 ggttcggagt tcagggaact gcgggtaatg ttcgaataag ccgctccctg agcacctcga
     2041 cttcgctcgg tataaactcc gccgaaggga gcaggtctga gacctgtccg cttcaaccaa
     2101 ggggttaacc ccttggttga caactcaact gacagattta tcattacaga atatgcctcc
     2161 gaacgccaga ctctgaaccc tgaaccccta ttaattatca ttacaaaaca ccctcc
//
LOCUS       DAOQGJ010000547         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32580_length_2216_cov_2.449329, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000547 DAOQGJ010000000
VERSION     DAOQGJ010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32580_length_2216_cov_2.449329"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PK683_09360"
     CDS             <1..312
                     /locus_tag="PK683_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08697.1"
                     /translation="AYGNGMYVAVTGTSSNAAASSPDGINWTSRTLPASQYWSSVTYG
                     NGIFVAVGNGSTTAAASTDGINWIAKTLPAAQTWKSVAYGNGVFVTVANGPTSSSASS
                     P"
     gene            586..1605
                     /locus_tag="PK683_09365"
     CDS             586..1605
                     /locus_tag="PK683_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH08698.1"
                     /translation="MTKFFTGIFFILLLVQCTTPDMKVDADEPEKDLFEDVIKNNIKS
                     VRKKLDSGRNVNARLLKNGNTLLLESIRREKPEIVRLLLERNADIHLEGTETALMFSI
                     HHPKSKAYRLLMQRGADISRRNYGALCVAVAQSTDEFASDLMKRLTDAGTILDRHCGG
                     LLGIRTKNRLEMIRTLASKGADISFKYAGGYQLIHSAAAKGDVELLKFLISKGADPNA
                     KEKTDDTDKTPLYYSLNFKYKAAADFLRQNKAEEYRDSEGRTFLHMEASRAAMQTKDV
                     IENIKFYAGAGIDRTLKDRNNKTAYDILSDYLNAEKDAGKKEISKEQTKYIAELTAVL
                     PKLKP"
     gene            complement(1602..>2216)
                     /locus_tag="PK683_09370"
     CDS             complement(1602..>2216)
                     /locus_tag="PK683_09370"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HNH08699.1"
                     /translation="LVGTPPMSVPHLDTRIINGKKELLFGPYAGFTTKFLKRGSYFDL
                     AKSLELGNIFPMISAGMHNFPLTRYLISQALQSHRDRVKALKEYFPGAKAKDWELVQA
                     GKRVQVIKSDEKEGGVLEFGTEVVAAKDGSLAAMLGASPGASTSVAIMLEVLTDCFKR
                     KTESPEWKERLKKLIPSYGESFLHNPELCERSRLRTSEILKLKL"
BASE COUNT          683 a          494 c          492 g          547 t
ORIGIN      
        1 gcctatggaa acggaatgta tgtggctgtc actggtacct catccaatgc agcagcaagt
       61 tctcctgacg gaattaactg gacgagcaga actctgcccg cttctcagta ctggtcatca
      121 gtcacttacg ggaatggtat ttttgtcgca gtgggcaacg gttcaactac agcagcagct
      181 tccacagacg gaatcaactg gatagccaaa accttgcccg ctgctcagac atggaagtca
      241 gtcgcatacg gaaacggagt ttttgttact gtagctaatg gtccaacctc atcatcggca
      301 agttctccgt agtggggatt ttgatgggaa tatattcgag tctcacctta cgcaaatacg
      361 atatgctgtc tagaagcggt aatttttaaa aacattagaa aaatctagca gtttaacagt
      421 ttgcgatgga tcagtaaaaa cgggagtttg agggggttag ccctcaagcc gccgacaggc
      481 gttcctgcca aaaaacgccc cacctgcgta aggtgaatta tgtaagttta tttgcagtga
      541 aggaaacacc ttgactcaat agtcatctgc tcagtatctg atcttatgac gaaatttttc
      601 acaggaattt tctttatcct tcttcttgtg cagtgcacta cacccgatat gaaagtggat
      661 gcagatgagc cggaaaagga tctttttgaa gatgttataa aaaacaatat aaaatctgtc
      721 agaaaaaaac tggactctgg caggaatgta aatgcaagac ttctgaaaaa cgggaacact
      781 cttctgctgg aatcgatcag aagggaaaaa cccgaaattg taagactgct tctggaaaga
      841 aatgctgata ttcatctgga aggaactgaa acagcactca tgttttccat tcatcatccg
      901 aaatcaaagg cctatcggct tctgatgcaa agaggcgcag acatcagcag aagaaactac
      961 ggtgctctct gtgttgcagt cgctcagagt acggacgaat ttgcgtcgga cctgatgaaa
     1021 cgtctgactg atgcaggaac cattctcgac agacactgcg ggggactgct cggaatcaga
     1081 acaaaaaaca gactggagat gataagaaca ctcgcatcaa aaggagcaga catcagtttc
     1141 aagtatgcgg gcggttatca gctcatccat tctgccgcag caaaaggtga tgttgaactt
     1201 ctgaagtttc ttatctcaaa aggtgcagac ccgaatgcga aggaaaaaac agacgatacc
     1261 gacaaaactc ctctttacta ttcattaaat tttaaataca aagcagctgc cgactttctg
     1321 agacagaaca aagcagaaga atacagagat tcagaaggaa gaaccttcct gcatatggaa
     1381 gcatccaggg cagcaatgca gaccaaagat gtcattgaga acataaaatt ctatgcaggg
     1441 gctgggatag acaggacact caaggacaga aataataaaa cagcatatga tattctttca
     1501 gattatctga acgcagaaaa agatgccggt aaaaaggaga tatcaaaaga acagacaaaa
     1561 tacattgcag aactgacagc agttctgcca aagctgaaac cttagagttt cagcttcagt
     1621 atttctgaag tcctcagtct gcttctctcg cagagttccg gattgtgcag aaaggattca
     1681 ccgtaactcg gaatcagttt cttcagtctc tccttccact ccggactttc cgtctttctc
     1741 ttgaaacagt ccgtcagaac ctcaagcata attgcaactg aagtggatgc ccccggagaa
     1801 gcaccgagca tagctgcaag agaaccgtcc tttgcagcaa ctacctctgt tccgaattca
     1861 agcacacctc cttccttttc atcggacttg atcacctgaa cccgtttgcc tgcctgaacc
     1921 agttcccagt cttttgcctt tgctcccggg aaatattctt tcagtgcctt gactctgtcg
     1981 cgatgagact gaagagcctg actgatcaga taacgggtca gaggaaaatt gtgcattccg
     2041 gcggatatca taggaaatat atttccaagc tccaaagatt ttgcgagatc aaaataagaa
     2101 cctcttttga gaaactttgt agtgaaccct gcgtacggac cgaaaagaag ttctttcttc
     2161 ccgttgataa tccttgtatc cagatgagga accgacatag gaggggtacc tacaag
//
LOCUS       DAOQGJ010000548         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32773_length_2209_cov_3.500929, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000548 DAOQGJ010000000
VERSION     DAOQGJ010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32773_length_2209_cov_3.500929"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..429)
                     /gene="rplP"
                     /locus_tag="PK683_09375"
     CDS             complement(16..429)
                     /gene="rplP"
                     /locus_tag="PK683_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNH08700.1"
                     /translation="MLSPKRVKFRKRQIGHLQGKDESGNKVSFGEYGLMAVTSGRLTA
                     RQIEAARISINRRIKRGGKIWIRIFPHIPVTKKPAETRMGKGKGNPEFWVAEIRPGRV
                     LFEMAGIEEEAARKALEMASFKLPIKTLFVKRTLV"
     gene            complement(442..1119)
                     /gene="rpsC"
                     /locus_tag="PK683_09380"
     CDS             complement(442..1119)
                     /gene="rpsC"
                     /locus_tag="PK683_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNH08701.1"
                     /translation="MGQKVNPIGLRLGITRTWDSIWFAKEDYKKNLHEDLKIREFLEK
                     RFKNSALVRVIIERFPEKINVNLHTAKPGVVIGQKGQNIESVKTQIKSFSDKPINLNI
                     VEVKKPEVVAQAIAETIATQMEQRMPFRRVMKAELRRAMRGGVEGIKISISGRLNGAD
                     MARIESYKDGRVPLHTLRAKLDFGFKEAKTTYGQIGIKVWTYSGDHIQNKSEETEDKY
                     AVKRRTD"
     gene            complement(1132..1464)
                     /gene="rplV"
                     /locus_tag="PK683_09385"
     CDS             complement(1132..1464)
                     /gene="rplV"
                     /locus_tag="PK683_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNH08702.1"
                     /translation="MEAKAVARYVRVSARKARLVANEIRGYDYLEAKDILKFTNKAVA
                     KIILKVLDSAAANAVVADSSVDETQLYVSKIYVDEGPMLKRFRARARGRAARIRKRQS
                     HITIVLSD"
     gene            complement(1464..1742)
                     /gene="rpsS"
                     /locus_tag="PK683_09390"
     CDS             complement(1464..1742)
                     /gene="rpsS"
                     /locus_tag="PK683_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNH08703.1"
                     /translation="MARSLKKGPFIDDHLMKKVTKMNSEGNLKPFKTWSRRSTIFPDM
                     VGHTVMIHTGNKFNPVYIRDTMVGHKLGEFAPTRTYRGHANQDKKVKK"
     gene            complement(1746..>2209)
                     /gene="rplB"
                     /locus_tag="PK683_09395"
     CDS             complement(1746..>2209)
                     /gene="rplB"
                     /locus_tag="PK683_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNH08704.1"
                     /translation="TLSLDKIPPGTNIHNVELHIGKGAQMARTAGGFAVVAAKDGDFV
                     TLKLPSSEVRKVRKECIATVGIISNKDHNLVSLGKAGRSRWLGRRPKVRGVVMNPVDH
                     PHGGGEGKTSGGRHPVSPWGQPTKGYKTRRKARPSDRFIVSGRKRNRNRGN"
BASE COUNT          511 a          544 c          395 g          759 t
ORIGIN      
        1 catgcagttt tactttcata caagagtcct cttcacaaat aatgttttga ttgggagttt
       61 gaaggacgcc atttcaagtg cctttcttgc tgcttcttct tcaatccctg ccatttcaaa
      121 caaaactctt cctggacgga tttcagcaac ccagaattca ggattccctt tcccttttcc
      181 catacgagtt tccgcaggtt ttttggttac aggaatatgc ggaaatatac gaatccaaat
      241 cttcccgcct cttttaatcc ttctgttgat cgaaatcctt gctgcttcga tctgtcttgc
      301 agtcaatctt ccggaagtga cagccatcag gccatattca ccgaaggata ccttgttgcc
      361 gctttcatct ttaccttgaa gatgaccgat ctgacgtttt ctgaatttta ctctttttgg
      421 agatagcata cttgtaccta atcaatccgt tctacgctta actgcgtatt tatcttctgt
      481 ttcttcagat ttattctgga tatgatctcc tgaataagtc caaactttaa tcccaatctg
      541 tccgtaagtg gttttggctt ctttaaaacc aaagtccagt ttagcacgaa gagtatgaag
      601 aggaacacga ccgtctttgt aactttcaat tctggccata tcagcaccat tcaaacgacc
      661 ggaaatcgaa attttgattc cctctactcc gcctctcatt gctcttctaa gttcagcctt
      721 cattactctt ctgaacggca tacgctgttc catctgagtg gcaattgttt cagcaatcgc
      781 ttgcgcaaca acttcaggct ttttaacttc tacaatattc aaattgatcg gcttgtcaga
      841 aaaacttttg atctgcgttt tcacgctttc aatattctga cctttctgcc cgatcacgac
      901 tccaggcttt gcagtatgaa gatttacatt gatcttttca ggaaatcttt caatgatcac
      961 tctgacaagt gcactgttct tgaatctctt ttcaagaaat tcacgaatct tcaggtcttc
     1021 atgaagattc tttttgtaat cttcttttgc aaaccagatg gaatcccaag ttcttgtgat
     1081 tcccagtctt aaaccgattg gattaacttt ttgtcccatt ctctttccct attaatctga
     1141 cagcactatg gtgatgtggc tttgcctttt tctgattctt gcggcacggc ctcttgctct
     1201 tgcgcgaaaa cgcttgagca tcggtccttc atccacataa atcttgctta cataaagctg
     1261 agtttcatct actgaactgt ctgctacaac tgcattcgct gcagcagaat ctaaaacctt
     1321 cagaatgatc tttgcaacag ctttgtttgt gaatttcaga atatctttgg cttcgaggta
     1381 atcgtagcct ctgatttcat tggcaacaag ccttgctttt cttgcagaaa ctctgacgta
     1441 tcttgcaact gctttagctt ccattatttc ttcaccttct tgtcctgatt cgcatggcct
     1501 ctgtatgtcc ttgtaggggc aaactcacca agcttatgac caaccattgt atctctgata
     1561 tacacaggat tgaatttgtt tcctgtgtgg atcattactg tgtgtccgac catatctgga
     1621 aatatagtgc ttcttctaga ccaagttttg aacggcttga gatttccctc agagttcatc
     1681 ttggtcactt ttttcatcag atggtcgtct atgaatgggc ctttttttaa acttcttgcc
     1741 atagattaat tgcctctatt cctgttacgt tttctgcccg aaacgataaa gcggtcactt
     1801 ggtctcgctt ttcttctggt cttataaccc ttagtaggct gaccccatgg gctgactggg
     1861 tgacggcctc cagaagtttt tccctctccc ccaccgtgcg ggtgatcgac cgggttcatc
     1921 acgacccctc tgaccttagg acgtcttcca agccatctgg aacggcctgc ttttcctaaa
     1981 gaaacaaggt tatgatcctt attgctgatg attccgacag tagcaatgca ctcttttctt
     2041 acttttctga cttctgaaga aggaagtttc agtgtcacaa aatctccgtc tttcgctgca
     2101 accactgcaa aaccgcctgc agttcttgcc atctgagcac ccttgccgat atgaagctcg
     2161 acattgtgga tgttcgttcc aggcgggatc ttatctaatg aaagtgtat
//
LOCUS       DAOQGJ010000549         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32906_length_2204_cov_3.063751, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000549 DAOQGJ010000000
VERSION     DAOQGJ010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32906_length_2204_cov_3.063751"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(498..1076)
                     /locus_tag="PK683_09400"
     CDS             complement(498..1076)
                     /locus_tag="PK683_09400"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNH08705.1"
                     /translation="MKKKILVIMGNPNRESFCAAVAEEYAGAAAKNFEVKKLFLSDLN
                     FDPILRAGFKSGQELEPDLKTAQELILWADHLVFVTPVWWGSVPALLKGFIDRTFLPG
                     FAFKYHSNDPWWDRLLSGRTARIIYTADSPKFWLWFSHRDAAIRTLKNATLEFCGVKP
                     VKTLCFPMLRKSTPEQRKKWLQKTKSVGEQGG"
     gene            1419..>2204
                     /locus_tag="PK683_09405"
     CDS             1419..>2204
                     /locus_tag="PK683_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNH08706.1"
                     /translation="MTTVESEPMKNSNGKERHILSVSGGKDSTALAIFMKQEYPDLDL
                     EYVFCDTGEELPETYAYIEKIETFLGKKITKLQSEKTWDEWVNQFSGYLPSARSRWCT
                     ANLKIIPFEKFVGEDTVYSYIGIRADENREGYISSRKNIIAKYPFKEHGINKEDVIRI
                     LNDSGLGLPDYYRWRTRSGCYFCFFQRKYEWLGLKENHPDLFEKAKKYETVNTDGSAQ
                     NFTWVQEGSLDVVLKNPDSIRKKHSQYETRVKEKKRRNSRKLID"
BASE COUNT          657 a          485 c          454 g          608 t
ORIGIN      
        1 attatgcaga caaggtcaga atattcaatc ttaattattt tctctgaact ttaggtttaa
       61 gtgagtttaa gacgcaagga actcctgctg caggctgact ttccgcctgt ctgaaaatac
      121 agtccagaat acaataccct tccagacaag ttaatacgac ttagtcactc accttacgcc
      181 tacggccgcc tacggctgcc tacggctgga ggtggggcgt ttttgacaga ggcctcggcg
      241 gtttaacctg cgaagggctc tgctctcgcc aacgtcagta accggctgcc aagctttatg
      301 aagatgactc ggcggctctg aaattgccaa aaacccgcaa gcctgaaagc tgctgttttt
      361 actgatccat tgcaagctgt taactgctgg attttcctaa tgttttctga aaatgaaagc
      421 atatggatag catctcgtat ttgcataagg tgtgtcagtc aggttgaatt aaatgattac
      481 tttaagagca gaaaaattta tccaccttgt tccccgacag atttcgtttt ctgcagccat
      541 tttttcctct gttcaggtgt gctttttcgg agcataggaa aacaaagagt tttcacaggc
      601 tttactccgc agaattccag tgtcgcattt ttcagggtac ggattgcagc atctctgtga
      661 gaaaaccaga gccagaattt aggagaatct gcagtataga taatcctcgc ggtcctgcct
      721 gagagaagtc tgtcccacca aggatcattt gaatgatatt taaatgcaaa tccaggaaga
      781 aatgtcctgt caataaatcc cttcagcagg gcaggaacac ttccccacca tacaggagtg
      841 acaaatacca gatggtctgc ccatagaatc agttcctgtg cagtcttcag atctggctcc
      901 agttcctggc ccgatttgaa accggctctc aaaatcggat caaaattgag atcagaaaga
      961 aagagttttt tcacttcaaa atttttagct gctgcgcctg catattcttc tgcgactgct
     1021 gcacagaaac tttccctgtt cggatttccc atgatcacaa gaatcttttt cttcattcat
     1081 tcacctgcaa tgttgtcaat gctcacatta agtaagaagc tgtcaagacg aaatgctttt
     1141 acttgcaccc gccacttgtc cattccaact gtcaaattcg atctgaaatt ttttcagatt
     1201 tgcagatatc tgcttttgct ttatctgtcc tgcaggacgt tcttgactcc cgatgatgtc
     1261 cagccgtccg gacagaccgt agtcccttcc caaattcaca gggagttccc atttccattt
     1321 tttgaatctg cctgcagcat ctttatcatt tgatttaatt gcaaaacccc agtcccaacc
     1381 tgcttgacag attagaatca ttttaaaaaa gtcatgttat gacgacagtg gaatcagaac
     1441 cgatgaaaaa cagcaatggg aaggaaagac atattctttc cgtttcgggc ggaaaggatt
     1501 caactgctct cgctattttt atgaagcagg aatatcctga tctggatctg gaatatgtat
     1561 tctgtgatac aggagaggaa cttcctgaaa cttacgccta tatagaaaaa atcgaaacat
     1621 tcctgggcaa aaaaatcaca aaacttcagt ctgaaaaaac ctgggatgaa tgggtgaatc
     1681 agttctcagg gtatctgcct tctgcgagaa gccgctggtg tacagcaaat ttaaaaatta
     1741 taccattcga aaaatttgta ggtgaggata cagtatacag ttacataggc atccgtgctg
     1801 acgaaaacag agagggctat atttccagca gaaagaatat catagccaaa tatcctttca
     1861 aagagcatgg aatcaataaa gaagatgtta tccgcatttt aaacgacagc ggtcttggac
     1921 ttcctgacta ttacagatgg agaactcggt ccgggtgcta cttctgtttc ttccagagga
     1981 aatatgaatg gctcggactg aaagaaaacc atcccgatct tttcgaaaag gcaaaaaaat
     2041 atgaaacagt caatacagac ggaagcgcac agaattttac ctgggttcag gaaggttcgc
     2101 ttgatgtcgt actgaaaaac cctgattcga tccgcaagaa acattcccag tatgaaacga
     2161 gagtcaaaga aaagaaacgc agaaacagca gaaaattaat tgac
//
LOCUS       DAOQGJ010000550         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32963_length_2202_cov_2.564509, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000550 DAOQGJ010000000
VERSION     DAOQGJ010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32963_length_2202_cov_2.564509"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PK683_09410"
     CDS             complement(<1..362)
                     /locus_tag="PK683_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08707.1"
                     /translation="MKAELSENTLNYTISSKTRGKLLAMILVGLISLALSFFLIHDPA
                     RGGHSNAGWSALLIGTFITLGVSVAGIFFTAITHITGSHWSVTIRRLTETFWIYLPIA
                     VVLLAIVTIFGTHDLYEWS"
     gene            complement(372..974)
                     /locus_tag="PK683_09415"
     CDS             complement(372..974)
                     /locus_tag="PK683_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH08708.1"
                     /translation="MKLNKTLLTFGFLPFIISCGLERGFPAWQFFPDMYESPAREAQE
                     FDPTAPNKIGGRIPPIGTIPVDFEPYEFDAVTSITDLPGKDKGLVRPADIKETLTSYK
                     RGEDRFQVNCFPCHGPRGQGNGPVNGPAPKMNVFPIPNLAGEDIAALTDGQLYHVITK
                     GWGRMPAYASQIEPDDRWKIIMYIRKIQEAYKSGKISDTK"
     gene            complement(977..1525)
                     /locus_tag="PK683_09420"
     CDS             complement(977..1525)
                     /locus_tag="PK683_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNH08709.1"
                     /translation="MYWFKKEQFHSYQETDSGVFGLFDTPAEILEAAKKTKEKGYDGF
                     DCLTPFPVHGLDDAMGLPRSGIPWISFFMGLVGCAFGFGFQYLTHAVDWQNNFSGKAM
                     NAWPAYVPITFEVTVFMAGTSAAIAMFYLAGLGKGRKSLHPDITSHRFALWIPSSSKN
                     YKQEEAVSFLGSLGAKEVTVVK"
     gene            complement(1525..>2202)
                     /gene="nrfD"
                     /locus_tag="PK683_09425"
     CDS             complement(1525..>2202)
                     /gene="nrfD"
                     /locus_tag="PK683_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNH08710.1"
                     /translation="LSTPLVLSVHTIVSFDFAVSILPGWHTTILPPYFVAGAIFSGFA
                     MVVTLMVIAREVFNLKEYITLKHLENMNKVIMVTGLMVGYAYGSEFFIAWYSGNEYEG
                     FTFVNRAFGPYAWAYWIMISCNVLSPQVFWVEKFRKNIKIMFVVSIFVNIGMWFERFV
                     IVMSTHKDFMPSSWAMYVPTFYDFAMLIGTFGIFFTLFLLFCRILPVIAIAEVKTVMP
                     SKDGGHH"
BASE COUNT          654 a          511 c          454 g          583 t
ORIGIN      
        1 gaccattcgt aaagatcatg ggttccgaag attgtcacaa tcgcgagaag cacgactgca
       61 atcggaagat aaatccagaa ggtttcagtc agtcttctga tcgtaacaga ccaatgagat
      121 ccggtgatat gagtaattgc agtgaagaaa atccccgcaa cggacactcc caaagtaata
      181 aatgttccaa tcaggagagc agaccatcca gcattgctgt gtccgcctct ggcagggtca
      241 tgaatcagga aaaaagacag tgccagactg atgagtccca ccagtatcat agcaagcagc
      301 ttccctcttg tctttgagga aattgtataa tttaaagtat tttcagaaag ttcagctttc
      361 ataattctcc gtcactttgt atctgaaatt tttccagatt tataagcttc ctgaatctta
      421 cggatataca taataatttt ccatctgtca tcaggttcta tctgagaagc atacgcaggc
      481 atacgacccc agccctttgt gattacgtga tacagctgac cgtcagtcag tgcagcaata
      541 tcctcaccgg ctaaattcgg aataggaaac acattcattt ttggagcagg tccattcaca
      601 ggtccgtttc cctgacctct cggtccgtgg catggaaaac agttgacttg aaatctgtcc
      661 tcgccccttt tataggatgt caaggtttcc ttgatgtccg caggacgaac cagacccttg
      721 tcttttcccg gaagatcagt gatgcttgtg acagcatcaa actcataagg ttcgaaatcc
      781 acaggaattg ttccgattgg aggaattctt cctcctattt tatttggagc tgtcggatca
      841 aattcctgcg cttcccttgc aggagattca tacatatctg gaaaaaactg ccatgcagga
      901 aatcctcttt cgagtccgca ggaaataatg aatggcagaa atccgaaagt aagtaatgtt
      961 ttattcagtt tcatttttat tttaccaccg taacttcttt tgcaccaagt gaaccgagga
     1021 agctgactgc ttcttcctgc ttgtaattct tggagctgga aggaatccaa agagcaaatc
     1081 tgtgacttgt aatatcggga tgaaggcttt ttcttccttt tccgagacct gccaggtaaa
     1141 acattgcaat cgctgcactt gttcctgcca taaacacggt cacctcaaat gtaattggca
     1201 catacgcagg ccaggcattc attgccttgc ctgaaaagtt gttctgccag tcgacagcgt
     1261 gagtcagata ctggaaccca aatccgaatg cgcatcctac aagacccata aagaagctga
     1321 tccaagggat gcccgatctg ggcagtccca ttgcatcgtc aagaccgtgc acaggaaaag
     1381 gagtgaggca gtcaaacccg tcatatcctt tttccttagt ctttttagct gcttccagaa
     1441 tctctgccgg agtgtcaaac agaccgaaga caccggaatc tgtttcctga taagaatgaa
     1501 actgttcttt tttaaaccaa tacatcagtg gtgtcctccg tctttacttg gcataactgt
     1561 ttttacttca gcaattgcga tcacaggaag aattctgcag aacagcagga acagagtgaa
     1621 gaaaataccg aatgttccaa taagcattgc aaaatcgtag aaagtaggca catacatcgc
     1681 ccagctcgaa ggcataaagt ctttatgtgt ggacatcacg attacaaatc tctcaaacca
     1741 catccctatg tttacaaaga tggatacgac aaacataatc ttgatgttct ttctgaattt
     1801 ttccacccag aacacctgtg gagaaaggac gttgcaggaa atcataatcc agtatgccca
     1861 ggcataagga ccaaatgctc tgttcacgaa agtaaatcct tcatattcgt ttccggaata
     1921 ccacgcgatg aaaaactctg aaccgtatgc ataaccgacc atcagaccgg tcaccataat
     1981 aaccttgttc atattttcca agtgcttgag tgtaatgtat tccttcaggt tgaacacttc
     2041 tcttgcaatg accatcagag tcacgaccat tgcaaaacct gagaaaatcg caccggcaac
     2101 gaagtaagga ggcagaattg tagtatgcca tccaggaagg atagaaaccg cgaagtcaaa
     2161 actgacgata gtatgcaccg aaagaacgag cggtgtagat ag
//
LOCUS       DAOQGJ010000551         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_32989_length_2201_cov_2.851351, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000551 DAOQGJ010000000
VERSION     DAOQGJ010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32989_length_2201_cov_2.851351"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..913)
                     /gene="prmC"
                     /locus_tag="PK683_09430"
     CDS             complement(50..913)
                     /gene="prmC"
                     /locus_tag="PK683_09430"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNH08711.1"
                     /translation="MEKDTILYVLTKSKEYLQSKGIETARLDAEVLLAHELKVQRINL
                     YSSFDRKLTEAEKDAYRVLIKERGSFRPVAYMIGRKGFYKHEFAVNENVLIPRPETEE
                     LVEFVLNCEEKDDALRILDMGTGSGCIGITLVDECPRWEMHFSDISPEALKIAEQNYS
                     AIIKKRIIEIPPHFYQSDLFESIPAENKYHFITFNSPYIPLEEKDRLMPDVLNHEPHL
                     ALFIINPQEFYKKLISQSRNWLENSGSLYLETDPQWIPLLQNLAESEGFQMTVKKDLS
                     KKDRFVRLRKK"
     gene            complement(913..>2201)
                     /gene="polA"
                     /locus_tag="PK683_09435"
     CDS             complement(913..>2201)
                     /gene="polA"
                     /locus_tag="PK683_09435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNH08712.1"
                     /translation="GEDADITFRLYKILEKELQSTGTMSVLDTIEQPLIPVLAAMETA
                     GVSIDTAYFKKLSKEYKDILNGLEKSIHKAAGKNFNIASTRELQQVLYEDLKLPVGKK
                     IQTGYSTDHSVLEDLQGKHEIIDFLLEHRKYSKLVSTYIDTLPLLVNPETGRIHTNYN
                     QTIAVTGRLSSTDPNLQNIPIKDKEGREIRKGFIADKDFEILSLDYSQIELRIMAHFS
                     KDEKMTDAYNKNLDIHARTASALFAVDEKKVTPEMRTKAKAVNFSVICGSTAYGLAQN
                     MRISRSEAGLFIDKYFLQYPGVKKFMEDICNFCFQNGYVETLTGRRRYVPDIKATNRQ
                     AIEAAKRIAINSPIQGTSADMIKLAMLRIHDELKKKKAKSRMIMQVHDELVFEVHKSE
                     KDLVFESAKKIMENVVRLDVPIIVQGKFGKNWDEAH"
BASE COUNT          538 a          477 c          449 g          737 t
ORIGIN      
        1 tcagttcaaa aatcaggata atgcagtacc gaaaggccgc tctttaaagt catttttttc
       61 tcagtctcac gaatcggtcc tttttggaaa gatctttctt gactgtcatc tgaaatcctt
      121 ctgattcggc aagattctgc agaaggggta tccattgagg atctgtttcc agataaagac
      181 ttcccgaatt ttccagccag ttcctggact gagatatcag ttttttatag aattcctgag
      241 ggtttataat gaaaagagca agatgaggtt catggttcag aacatccggc atcagtctgt
      301 ccttctcttc cagaggaata tagggtgaat tgaatgtaat gaaatgatat ttattttctg
      361 caggaatgct ttcaaacaga tcactttggt aaaaatgcgg aggaatctct atgattcttt
      421 tttttatgat tgcagagtaa ttctgttctg ctattttcaa ggcttcagga gagatgtctg
      481 aaaaatgcat ttcccatctg ggacattcat ccacaagtgt aattcctatg catccggaac
      541 ccgttcccat atccagaatg cggagtgcat catctttttc ttcacagttc agaacaaatt
      601 ccacaagctc ttccgtttcc ggtctgggga tcagaacatt ttcattcact gcaaattcat
      661 gcttgtagaa gccctttctt ccaatcatat aggcgactgg acgaaagctt cctctttcct
      721 tgatgagaac cctgtacgcg tctttctctg cttcggtgag cttccggtca aagctggagt
      781 agaggttgat tctctgcact ttgagttcat gggcaagcag aacttctgca tcgagtctgg
      841 ctgtttcaat tccttttgac tgaaggtatt ccttcgactt ggtcagaaca taaagaattg
      901 tatctttttc catcaatgag cctcatccca attctttccg aatttcccct gaactatgat
      961 cggcacgtcg agacgtacaa cgttctccat gatttttttg gcggattcaa atacaaggtc
     1021 tttctccgat ttatggacct cgaacacaag ttcatcatgc acctgcataa tcatcctgga
     1081 ctttgccttc ttctttttca gctcatcgtg aatccgaagc attgcaagtt tgatcatatc
     1141 tgcggaagtt ccctggatcg gactgttgat tgcaatcctt tttgctgctt cgattgcctg
     1201 tctgtttgtg gctttaatgt ccggtacata tcttcttctg cctgtgagcg tttccacata
     1261 accgttctga aagcagaaat tgcagatgtc ttccataaat tttttcactc caggatactg
     1321 cagaaaatac ttgtcgatga acagtccggc ctccgatctg gaaatcctca tattctgggc
     1381 aagaccgtaa gcagtggaac cgcagatgac cgaaaagttc actgcctttg ccttggttct
     1441 catttccgga gtcacttttt tttcatctac cgcaaaaagc gcactggctg ttctggcatg
     1501 aatgtccaga tttttattat atgcgtccgt cattttttcg tctttcgaaa aatgagccat
     1561 gatcctgagt tctatctgag agtagtccag actgagtatt tcaaaatctt tatctgcaat
     1621 gaatcccttc cggatctctc ttccttcctt gtccttgatc ggaatgttct gaagattcgg
     1681 atcagtggaa gagagtcttc ctgtcactgc aattgtctga ttgtaatttg tatgtattct
     1741 tcctgtctca ggatttacga ggagagggag agtgtcgata taggtagaga caagtttgga
     1801 atattttctg tgttccagaa gaaaatcaat gatctcatgt ttcccctgaa gatcttccag
     1861 cacgctgtga tcagtggaat atcctgtctg tatttttttg cctacaggaa gtttcagatc
     1921 ctcataaaga acctgctgca gttctcttgt agatgcaata ttgaaatttt ttccagcggc
     1981 tttgtgaatt gatttttcga gaccgttcag tatatctttg tattctttgg aaagtttttt
     2041 gaaatatgct gtatctatgg aaacgccggc agtctccatt gcagcaagga caggtatcag
     2101 aggctgctca attgtgtcca gtacagacat cgttcctgtg gactgcagtt ccttttccag
     2161 tattttatag agacggaaag tgatgtctgc atcctctccc g
//
LOCUS       DAOQGJ010000552         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33023_length_2200_cov_2.236830, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000552 DAOQGJ010000000
VERSION     DAOQGJ010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33023_length_2200_cov_2.236830"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1387
                     /locus_tag="PK683_09440"
     CDS             92..1387
                     /locus_tag="PK683_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH08713.1"
                     /translation="MKKSFISSGTHTDRLNRTLADFTVQRRQTDDIFSLCTDLYESIE
                     YKKAYGHHHYGRVIHSKNSDSSVVEDPVTSDHKEMIMMGSNSYLGLHIHPRVISAAKN
                     ALDRYGAGAGSPPHFSGTYDIHRELEKNLALLKGTEDAIVFPSGYSTNVGILSAFLSS
                     NDTLIIDKFAHASIIDGGLLSRARIASFRHNDMESLEKTLIRTRNSSGDRLVAVEGVY
                     SVDGDIAPLNEIYSLVKKHDAKLMVDDAHGTGVLGKTGRGTAEYFDLEGEIDLVMGTF
                     SKALGGIGGFVCAKKEIIRYLRFYSRSYFFAAAVSPVQAAALNEAVLVLTEEPEWHRR
                     LNDNINFFHSELRNRGFNLENCRTAITPVIIGDSFKTEEVTNFLHERGVFVNCVPYPA
                     SPRKKERIRMSVSALHKKEDLMTVIRLLTEARDLFHFQN"
     gene            complement(1378..>2200)
                     /locus_tag="PK683_09445"
     CDS             complement(1378..>2200)
                     /locus_tag="PK683_09445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNH08714.1"
                     /translation="LAEGVFLILVGLSKKLAADWIGSNIVNPVYSNPELFSSAETWTA
                     VYAYGVQIYGDFSGYTDIAIGSAMLLGFHLTVNFNSPYISCSVSEFWRRWHMSLGSWL
                     RDYLYIPLGGSRERVYRNLILTMFLCGLWHGAGWNFILWGLYNGLLMAFERLVYKDGQ
                     KKYKFSFFRWLLTFHLIIFGWIVFRSENMTQFMKIWNGLFAGRSDFVNVPMYSVGIII
                     IIYLLQFIPEKTQRRISRFWKELPALISAFAVSVFIVLMHNLRNFEMKQFIYFQF"
BASE COUNT          698 a          464 c          460 g          578 t
ORIGIN      
        1 gtgcaaaagc tgatttcggc tttcaggctg aaacaaaact ggaagacgga attgcagaaa
       61 cactcaaatg gtataaagaa aacagatggc tttgaaaaag tctttcatca gcagcggaac
      121 acatacagac agactgaaca gaaccctggc cgatttcact gtacagcgcc gtcagacaga
      181 cgatatattt tccctctgta ccgatcttta tgaaagcatt gaatataaaa aagcttacgg
      241 acaccatcac tatgggagag tcattcattc taaaaactcg gacagctctg ttgtagaaga
      301 tccagttact tcagatcata aagaaatgat catgatgggt tccaattcct atctgggtct
      361 tcatattcat ccaagggtca tctctgctgc aaaaaatgca ctggacagat atggggcagg
      421 cgcaggttct cctcctcatt tttccggaac atatgatatt cacagagaat tggagaaaaa
      481 tctggcgctt ctcaaaggta ccgaggatgc aattgtattt ccatcaggat attctactaa
      541 tgtagggatc ctttctgcct tcctaagcag taatgatact ttaattatag ataaatttgc
      601 ccatgcttcc atcattgacg ggggacttct ttccagagcc agaattgcct ctttcagaca
      661 caatgatatg gagtccctgg aaaaaacact gatcagaaca aggaacagtt ccggggacag
      721 gctggttgca gtggaagggg tctattctgt agacggtgat attgctcctt tgaatgagat
      781 ttactccctt gtgaaaaaac acgatgcaaa gctgatggtg gatgatgctc acggaacagg
      841 agttcttgga aaaaccggca gaggcactgc agaatatttt gatctggaag gagaaattga
      901 tctggtgatg ggaactttca gcaaggctct tgggggaata ggaggatttg tatgtgcaaa
      961 aaaagaaatt atccgttatc tgcgttttta ctcaagatct tatttttttg ctgctgcagt
     1021 ctctcccgtt caggctgcag cactgaacga agcagtgctt gttctgacgg aagagccgga
     1081 atggcataga cgtcttaatg ataatataaa tttttttcat tctgaactca gaaaccgagg
     1141 tttcaacttg gaaaactgcc ggaccgccat tacccctgta atcatcggag acagtttcaa
     1201 aacagaagaa gtgacaaatt tcctgcatga gagaggagta tttgtgaact gcgttccata
     1261 tcctgcatca cccagaaaaa aagaaagaat cagaatgtca gtttctgccc ttcacaaaaa
     1321 agaagatctg atgacggtca tcaggctgct cactgaagca agagatcttt ttcattttca
     1381 aaactgaaaa taaataaact gcttcatttc aaaatttctc agattgtgca tcagaacaat
     1441 gaatacagaa actgcaaaag cagaaatcag agccgggagt tctttccaga atctggaaat
     1501 ccttctctga gttttttctg gaatgaactg cagaagatat attataatta taataccgac
     1561 agagtacatc ggaacattta cgaaatctga ccttcctgca aaaagaccgt tccatatctt
     1621 cataaactga gtcatatttt ccgaccgaaa aacaatccat ccgaaaatga tcagatgaaa
     1681 tgtgagcagc catctgaaaa agctgaattt gtattttttc tgaccgtctt tgtataccag
     1741 acgctcaaaa gccatcagca gaccgttata cagtccccat agaatgaaat tccagcctgc
     1801 tccgtgccag agaccgcaga gaaacattgt cagtatcaga ttcctgtaca ccctttccct
     1861 gcttcctccc agaggaatat acagatagtc tctgagccag gacccgagag acatatgcca
     1921 tcttctccag aattcactga ctgagcagga gatgtaagga gaattaaaat tcacggtgag
     1981 atgaaatcca agaagcattg cagatccaat tgcaatatca gtgtatccgg aaaaatcacc
     2041 gtagatctgc actccgtatg catacactgc agtccaggtc tctgctgaac tgaaaagttc
     2101 cggattggaa tatactggat ttactatatt cgaaccgatc cagtccgcag caagtttctt
     2161 cgaaagaccg acaagtatga ggaagacacc ttctgcaagt
//
LOCUS       DAOQGJ010000553         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33081_length_2197_cov_3.567694, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000553 DAOQGJ010000000
VERSION     DAOQGJ010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33081_length_2197_cov_3.567694"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK683_09450"
     CDS             complement(<1..264)
                     /locus_tag="PK683_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08715.1"
                     /translation="MNLNRQLYYSLIIYILIFFAVSCRTEKTDLLSGSGSIEVNEIHI
                     ASIVPGRIEKISVDEGDSFVKEETLVVIKGDEIQADLDAAGAGL"
     gene            complement(261..1616)
                     /locus_tag="PK683_09455"
     CDS             complement(261..1616)
                     /locus_tag="PK683_09455"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH08716.1"
                     /translation="MKKEFSVLLLTVCLPVYSEKAKLLTLSDCVEIGLKNNIGIRIQK
                     SDLAISQYQKEIAESSQLPDFSAAAGYTYIDPALKGRIEMVPGTDLALKPRLGFHNNY
                     SLALQMRYVLYSGGKISRAEEAAQLGEEAAKLLLKDRLQSSKYEIRKAFIQAVFLKEM
                     VHLAEESLKRSSDRLKDTEAKFKAGSVTKIEMLRIRAENSESETFFSETRDRYKAGTD
                     QLKLLLNLPIKEEIEISGSLKIHPDELKRVFSLNLSAFTPVFWKAQAAGIQAKQAGKL
                     SEMEKSDMLPAVAVGMKADEANPYLLQDKFGTIYSGFIQASMPVWDWGKNKKKYLISQ
                     EQKIKAESIAEDVRNQLEAYYSILTDRIQSIKKGINSREKNLDRSKAARDAAETARKN
                     GAATYNDWADTELLHFRLEVDYLKNLSDLYISIADLERFIEEPTDIFSGFETDSQRKN
                     R"
     gene            complement(1675..>2197)
                     /locus_tag="PK683_09460"
     CDS             complement(1675..>2197)
                     /locus_tag="PK683_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH08717.1"
                     /translation="TSMIAEKAGVAEGTIFKHFKSKKDLLVQITVPVITKLFLPVAFQ
                     AVNKVFKGNYSSFREFLTYFITERIMFAGKYSKYLKILVQEIPVNEDLREFIIKEFRK
                     KVWPNLKELIESFQKKGELTKMPPEAVFSCMISVGGAYLYRRIILLPREVYDDKKDIE
                     MLVNHILFGISAK"
BASE COUNT          541 a          509 c          416 g          731 t
ORIGIN      
        1 aaggcctgca cctgctgcat ccagatcggc ctgtatttca tctcccttga tcacgacaag
       61 agtttcttcc tttacaaaac tgtctccctc atcaactgaa attttctcaa tccttcctgg
      121 aacaatcgaa gcaatatgaa tttcattcac ttcaattgaa cctgaccctg aaagaagatc
      181 tgtcttttct gttctgcagg agactgcaaa aaatataaga atgtatatta ttaaagaata
      241 atacaattgt ctgttcaagt tcatctgttc ttcctttgag aatctgtttc aaatcctgaa
      301 aaaatatccg tcggctcctc tataaatctt tccagatcgg ctatggaaat atacagatcg
      361 gaaagatttt tcagataatc cacttccagc cggaaatgca ggagttcagt gtctgcccag
      421 tcattgtatg tagcagctcc gttctttctc gctgtttcgg ccgcatccct tgcagctttt
      481 gatctgtcca gattcttttc tctggaattg attccctttt tgatgctctg gattctgtca
      541 gtcagaatgg aataatatgc ttcaagctga tttctgacat cttctgcgat ggactcagcc
      601 tttattttct gctcctggga tataagatat tttttcttat tcttccccca gtcccagacc
      661 ggcattgacg cctgaatgaa tcctgaataa attgttccga atttgtcctg caggagatac
      721 ggatttgctt catccgcttt cattcccact gccacagcag gaagcatatc cgacttttcc
      781 atctccgaaa gcttgcctgc ctgttttgcc tgaattcctg cagcctgtgc tttccagaac
      841 accggcgtga atgcgctgag attcagagag aacacccgct tcagttcatc cggatgaatc
      901 ttcagagacc cgctgatttc tatttcttcc ttgatcggca gattcagcag aagtttgagc
      961 tgatctgttc ctgccttgta tctgtccctg gtctcggaga aaaatgtttc agactcggaa
     1021 ttttctgcac gtatcctaag catttctatt tttgtcacac tgcctgcctt gaattttgct
     1081 tcagtgtcct tcagtctgtc tgaacttctt ttcagtgatt cttcagcaag atgaaccatt
     1141 tctttcagaa agactgcctg aatgaatgcc tttctgattt cgtactttga tgactgcagt
     1201 ctgtctttca gcagcagttt ggctgcttct tctcccagct gggcagcttc ttcggctctg
     1261 ctgatttttc cgcctgagta taacacatac ctcatctgaa gtgcaagaga gtagttgttg
     1321 tgaaagccca gcctgggttt cagtgcaagg tcagttccag gcaccatctc tatcctgcct
     1381 ttgagagcag ggtcaatata tgtataacct gcagctgcac tgaaatcagg aagctgactg
     1441 gattcagcaa tttctttctg gtactgactg attgcaagat cggatttctg aatgcgtatt
     1501 cctatattgt tcttcaggcc tatttctacg cagtctgaga gggtcagcag tttcgctttt
     1561 tccgaataga ccggcaggca gacagtcaga agcagaacag agaattcttt tttcattgtt
     1621 tctcctttga tgagtgagta atcactcagt tttataacca aaaataaatt tcgattattt
     1681 tgccgatatt ccaaacagga tatgattcac cagcatttct atgtcttttt tatcatcata
     1741 aacttcccta ggaagaagaa tgattcttct gtacagatat gctccgccta cagagatcat
     1801 acaggagaat actgcttcag gaggcatttt tgtcagctca ccttttttct gaaaactctc
     1861 tatgagttcc ttcagattcg gccatacctt ctttctgaat tcctttataa taaattccct
     1921 tagatcttcg ttgaccggaa tttcctgcac tagaattttc agatacttgc tgtattttcc
     1981 ggcaaacata atccgttcgg taatgaaata agtcagaaat tctctgaatg aagagtaatt
     2041 tcccttgaaa accttgttga ctgcctgaaa tgcgaccgga agaaacagtt ttgtgatgac
     2101 tggaacagtg atctgaacaa gaagatcttt ttttgatttg aaatgcttga agattgtgcc
     2161 ctcagctacg cctgctttct ccgcaatcat gcttgtg
//
LOCUS       DAOQGJ010000554         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33117_length_2196_cov_2.444185, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000554 DAOQGJ010000000
VERSION     DAOQGJ010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33117_length_2196_cov_2.444185"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK683_09465"
     CDS             complement(<1..656)
                     /locus_tag="PK683_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH08718.1"
                     /translation="MKDFQKPFYILAAVFIAYAAFTVKPLIRFFSAENYVTIDGKKYT
                     EADLADNPIYKKVRKGYIEGLSQAFQAFAAEEIVKNEAKSKGMDEKEFLRSSVSKPSE
                     AEILQVFEQAAPRLGGKKLEEVRSQIVEYLANMKEDQFRRGLSEKYKIAIHVEKPGRQ
                     KVDEKNNPTLGPKDAKVTIVEFSDFECPYCNKSQEVNRALRDKYKDKIVLIGLTAAGV
                     G"
     gene            1160..1861
                     /locus_tag="PK683_09470"
     CDS             1160..1861
                     /locus_tag="PK683_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08719.1"
                     /translation="MKLFLIVIFISVSAFSQENSEEYSEFLEFKEYQKDKNKNPETEE
                     EKEKEYREFLKARKNRKEKSDPSLSIPENKTQKWALIFGFGSRTLKSLSYTGTSGDLS
                     IFAEYLAPKTNLGFLFGVGYQNYKITGSNQTSFAEPLTLDLGMSWHFFPESDLDLYLA
                     GKIGFGTLGDSGAFWKASISPGLRFFLNKTEKDRGEFLFLEGILEKPYFSSQNSPFQE
                     DFYAYGLRIGIGTWL"
     gene            1864..>2196
                     /locus_tag="PK683_09475"
     CDS             1864..>2196
                     /locus_tag="PK683_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08720.1"
                     /translation="MKRIFAVLLFLTVLNFVFPQSGESWEEQNLKEFEEFKRMREKEK
                     KTEAEKKAKDREDDHNSGLFLSFGGGTGNILSSGFERKKMEQAGFSIHYLQKNSHLGF
                     RISVGGWNY"
BASE COUNT          613 a          437 c          473 g          673 t
ORIGIN      
        1 ccgacaccgg ctgcggtcag accgatcagg acaattttgt ccttgtattt gtcgcgcagt
       61 gccctgttga cttcctgact cttgttgcag tacgggcatt caaagtcgga aaattcgacg
      121 attgtcactt ttgcatcttt aggaccgagg gtaggattgt ttttttcatc caccttctgt
      181 cttccaggtt tttcaacatg aattgcaatt ttatatttct cggaaagacc tcttctgaac
      241 tgatcctcct tcatattggc cagatactcc acaatctgag atctgacttc ctccagtttt
      301 tttcctccga gtctcggtgc agcctgctca aatacctgca gaatttccgc ttcggaaggt
      361 ttggatacgg aggaacggag aaattctttt tcgtccattc ccttggattt tgcttcattc
      421 tttacaattt cttcggcagc aaatgcctgg aatgcctgag aaagcccttc aatgtaacct
      481 tttcttactt tcttgtaaat aggattgtct gcgagatctg cttcggtata ctttttcccg
      541 tctattgtaa cataattctc cgcactgaaa aatctgataa gcggtttaac tgtgaatgca
      601 gcgtaggcta tgaatactgc tgctaaaata taaaagggct tttgaaaatc tttcattaaa
      661 gaaactccat ctatactaat tttttttcag agtgataggt tacaaacaaa aaaacattcc
      721 ctgactctga gctgctgtcc ggctttgcct gatgactgct gtctctggtc aaaggacggg
      781 tgttgagctg ccagagtaag atttttaacc ctcatcgaac atatggcagc caaaacgttt
      841 tcaaaaagct aaggaagtcc tcttgatgct ccttgggctg taagatactg gttccaggaa
      901 gcaagctgca tagacttttt aagcgattgg atcaattctt attattttcc gatctgaaaa
      961 cccgttaaga tcttggtaag atgccgactt tgcaagttcc caggctcctg aatttcccaa
     1021 ttcctaaaaa tgcaggtgcc gagcattcta tttttttaag ttgcagtgag gtagatcgca
     1081 tttctttgtt tcaatacgtt ttcagaaagg gatattgcaa ataatttgac gatgctcggc
     1141 ttagtttttt tatataagta tgaagttgtt tcttattgtc atttttattt ccgtttctgc
     1201 tttttctcag gaaaactctg aggaatacag tgaattttta gaatttaaag agtaccagaa
     1261 ggataaaaac aagaatccgg aaaccgaaga ggaaaaagag aaagaataca gggagttttt
     1321 gaaagccaga aaaaacagga aggagaaatc agatccttct ctgagtattc ctgaaaacaa
     1381 gacccagaaa tgggcactga ttttcggttt cggaagcaga acgctgaaaa gcctttccta
     1441 tacaggcact tccggtgatt taagcatttt tgcagaatac ttggctccaa agacaaatct
     1501 gggatttctc ttcggggtcg gatatcagaa ttataaaatc acaggatcga atcaaacctc
     1561 ctttgcagaa cccctcactc tggatctggg gatgtcctgg catttttttc cggaatcgga
     1621 tctggatctt tacctggcag gaaagatcgg tttcggcact ttaggggata gcggagcatt
     1681 ctggaaggct tccatttctc ccggattacg attttttctg aacaaaacgg aaaaggacag
     1741 gggagagttt ctttttctgg aaggcattct ggaaaaacct tatttttcca gtcaaaattc
     1801 tccgtttcag gaggatttct atgcatacgg tcttcgaatt ggaatcggaa cctggctttg
     1861 agtatgaaac gaatctttgc ggtactgctg tttttgactg tcttgaactt tgtttttcca
     1921 cagtcggggg aatcttggga agaacagaac ctgaaagaat ttgaagaatt taaaagaatg
     1981 agggaaaaag agaagaaaac ggaagcggaa aaaaaggcaa aggacagaga agacgatcat
     2041 aacagcggtc ttttcctttc cttcggagga ggaaccggca atatcctcag ttccggcttt
     2101 gaaagaaaaa aaatggaaca ggcaggattt tccatccatt atcttcagaa aaattcccat
     2161 cttggatttc gaatctcggt cgggggctgg aattac
//
LOCUS       DAOQGJ010000555         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33136_length_2195_cov_2.673364, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000555 DAOQGJ010000000
VERSION     DAOQGJ010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33136_length_2195_cov_2.673364"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /gene="mraY"
                     /locus_tag="PK683_09480"
     CDS             <1..708
                     /gene="mraY"
                     /locus_tag="PK683_09480"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNH08721.1"
                     /translation="FISVILAGAFCILYFYFTSGIVKDGKGIHFDISDIFFPFIKGPV
                     FNLGILIVPFSALIIISSSHGVNLTDGLDGLAAGTVAIAASCLAVIAYVSGTPVAANY
                     LNIPYLPGAHEYSVFLAAMVGSLLGFLWFNAHPAEVFMGDTGSLFLGASIGMVSVLLK
                     KELLLPILGGIFVMESLSVILQVGSFKLRQKRIFLMAPIHHHFELKGWKETKVVYRFY
                     IIAIILALISLSTLKIQ"
     gene            772..1863
                     /locus_tag="PK683_09485"
     CDS             772..1863
                     /locus_tag="PK683_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNH08722.1"
                     /translation="MHIVYTVLSLILLGLCILFTSSMVTSQRDYNDPYFFLKKQSAWA
                     VISLVFFAVFSLIPYKIYQKVSVIILLFSFLLLSLVFIPGVGKTVDTHYGRIFHRWIR
                     IGSYQFQPSEFVKIVMIIYVSDILCRWKTVWNMSYRHLAPPLFAVGVCLGMIVAEPAF
                     GTAMEILLIILVLFFVDGFPLLRIFAAILSLSPLAAILVYKVGYRKKRVDVWLDPYKY
                     RFEEGHQLVSSFRSFLDGSWFGQPLTTGYSHRHLTYGHTDFILAPLVEDYGFVGFLCI
                     SILTFYLIFRSVILLRKMKEGFGLLLGTGITGMIALQFLLNTFVVTGIVPITGISLPF
                     ISYGGSSLLSVMIGCGILLNITRKENLSA"
     gene            1860..>2195
                     /locus_tag="PK683_09490"
     CDS             1860..>2195
                     /locus_tag="PK683_09490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH08723.1"
                     /translation="MKNILIAAGGTGGHISPGIALAEEICRKKQDFGIDNVFIHSLKR
                     NIDNPDLKDSPCPVLWHSTPQPSVLNAFSILFSALREISVLRKRKITHVIAMGGYSCI
                     PAVLYSVFFR"
BASE COUNT          524 a          468 c          477 g          726 t
ORIGIN      
        1 ttcatttcag tgattttggc aggtgctttc tgcattctgt atttttattt tacttcgggc
       61 atcgtgaagg atggaaaggg aattcatttt gatatttcag acatattctt ccctttcatc
      121 aaaggtcctg ttttcaatct ggggatactt attgttcctt tctctgcact gatcataatc
      181 agctcttcac atggagtcaa tctgactgac ggactggacg gtctggctgc aggcacagtt
      241 gcaattgctg cgtcctgtct ggctgtcatc gcctatgtat ctggaacacc agttgcggcg
      301 aattacctga atatccctta tcttcccggt gcacacgaat attcagtttt tctcgctgcg
      361 atggtaggat ctctgctggg ttttttatgg ttcaatgccc atccggcgga agtttttatg
      421 ggggataccg gatcgctgtt tctgggagct tccattggaa tggtctcggt gcttctgaaa
      481 aaagaacttc ttcttccgat actgggagga atctttgtaa tggagtctct gagtgtcatt
      541 cttcaggtcg gatcgttcaa actcagacag aaaagaattt tcctgatggc accgatacac
      601 catcattttg agctgaaggg ctggaaggaa accaaggtgg tatacaggtt ttacattatt
      661 gcaattatcc ttgcactgat cagcctctcc actctcaaga ttcagtagaa attgaaacga
      721 atcagggacg cactttcgag actttttgaa ccgggaaaaa atgcagtgga tatgcatatt
      781 gtctacactg tactttctct gattcttctc ggactctgca ttcttttcac atcttccatg
      841 gttaccagcc agagagatta taatgatcct tactttttcc tgaaaaaaca gtcagcctgg
      901 gcagtgattt ctctcgtatt tttcgcagta ttctctctga ttccttataa aatctatcag
      961 aaagtttctg taattatcct tctgttcagt tttcttctgc tttctcttgt attcattccg
     1021 ggagtaggaa agactgtaga tactcattac ggccggatat ttcacaggtg gatccgtata
     1081 ggcagttatc agtttcagcc ttccgagttt gtaaaaatcg taatgatcat atatgtatcg
     1141 gatattctct gcaggtggaa aactgtctgg aatatgagtt acaggcatct ggcgcctccg
     1201 ctttttgcag tcggtgtctg tcttggaatg attgtggcag aacctgcatt cggaactgca
     1261 atggagattc ttcttatcat ccttgttctt tttttcgtgg acgggtttcc tcttctcaga
     1321 atttttgctg caatcctttc tctttcccct ctggcagcca tactggtata taaagtggga
     1381 tacagaaaaa aaagagtcga tgtatggctt gatccttaca agtacaggtt tgaagaggga
     1441 catcagcttg tttcctcctt ccgttccttt ctggacggtt cctggttcgg tcagcctctg
     1501 accaccggct actctcacag acatcttacg tacggacata cagactttat tctggctcct
     1561 ctggtggagg actacggctt tgtcggtttt ctgtgcatat cgattctgac tttttacctg
     1621 attttcagat cagtcatcct cctcaggaaa atgaaagaag gattcggtct tcttctgggt
     1681 acaggcatta cgggaatgat cgcacttcag tttcttctga atacatttgt tgtgacagga
     1741 attgtcccga ttacgggaat cagtcttcct ttcatcagtt acggaggctc gtctctgctt
     1801 tctgtcatga tcggatgcgg aattttatta aatattacaa gaaaggagaa cctttcggcg
     1861 tgaagaacat actgatagca gcaggaggaa ccggggggca tatttcaccg ggtattgcac
     1921 tggctgaaga aatctgcaga aagaagcagg attttggaat tgataatgta tttatacatt
     1981 cattaaaaag aaatatagat aatcccgatc tgaaggacag tccgtgtcct gtgctctggc
     2041 acagcactcc ccagccttca gtattaaatg cattttccat tctcttttca gcacttagag
     2101 aaatttcagt tctcaggaaa aggaaaatta cccatgtgat cgcaatgggc ggatattcct
     2161 gcattcctgc agttctctat tccgtatttt tcaga
//
LOCUS       DAOQGJ010000556         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33158_length_2194_cov_3.076671, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000556 DAOQGJ010000000
VERSION     DAOQGJ010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33158_length_2194_cov_3.076671"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="PK683_09495"
     CDS             <1..1163
                     /locus_tag="PK683_09495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH08724.1"
                     /translation="SNLNYFRTRQSRFGLSGFYIRLPFSGKWKISQGYDGKFTHKEQW
                     RESLDFMAADDAGKVRKGQEDSVKDFYTFGLPVLAPAAGRVIKVVSHLEDSRLGEADT
                     KNNWGNMVLIEHSLFLYSQVSHLQQNSVTVKEGDYVSAGFKLACAGNSGRSFEPHIHL
                     HFQKTPEIGSPTCEAGFSQYLNYNDKTSEVSFNSIPKENEIISHLNSDFNLKNFFTLA
                     PGQKLKIQTEAKSSVQKEKWTVNLDLFGNRFIEDESQNRLYYISTQDYFACIDYIGRR
                     GSNLFMMYLSLYRIPFLSMETSWRDTISYTHFSNPLGVFFKDLISPFHSGISYDWFGS
                     IKKEGQSEFITAEVIKSGIKIFESQCIPGNSAFPGKIKVSFGGKECSLSITE"
     gene            1139..>2194
                     /locus_tag="PK683_09500"
     CDS             1139..>2194
                     /locus_tag="PK683_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08725.1"
                     /translation="MQSVHHRIKKLTVYILIFLITLSASAKTVEEAYALIEKGNPDKA
                     VDILQKIIQKNPKDYAAHLTLGYAHMLKERYAKALESYRKADEANSTLDSLSGIQWAY
                     LALGQHDDSIKIGEKILEKDPKNFYARYRTAESLLAQKKYDSAAKYYRSLDSDFPKNQ
                     DVLWKLGLCELYQGRMDASADYFQKMLQENPNSYSAHLNLGYINLMKERPAKALEHYR
                     KADEIYSGLDSQSGIQWSLLALGKHDESIRMGEKILEKDPKNYFARYRIAESRLAQKD
                     YENAGKQYKSLQADDPKNTDILWKLGLCEYYQGNTSRANSYFEEGLKNDPKHKGLRYS
                     LGLPTTPSVEVSPEYTTY"
BASE COUNT          710 a          465 c          474 g          545 t
ORIGIN      
        1 aaagcaatct gaactacttc agaacaagac agtccagatt cggcctctcc ggcttttaca
       61 tccgtcttcc tttcagcggc aaatggaaaa tatcgcaggg ttatgacgga aagttcactc
      121 acaaggaaca gtggagagaa agtctggact ttatggctgc ggatgatgca ggcaaggtaa
      181 gaaagggtca ggaagattcc gtaaaggact tttacacatt cggtctgcct gtacttgcac
      241 ctgcagcagg aagggtaatc aaggtcgtca gccatctcga ggacagcaga ctcggagaag
      301 cggatacaaa aaacaactgg ggaaatatgg ttctgattga acattctctg tttctgtatt
      361 ctcaggtctc tcatctgcag cagaattcag tgactgtaaa ggaaggcgac tatgtaagtg
      421 cgggattcaa gcttgcctgc gcaggaaatt caggaagaag ttttgaacct catattcatc
      481 ttcatttcca gaaaacaccg gaaataggaa gcccgacctg cgaagccggt ttttctcagt
      541 atttaaatta taatgataaa acatccgaag taagcttcaa ttcgattccg aaagagaatg
      601 aaatcatcag tcatctcaat tctgatttca acctgaagaa ctttttcact ctcgctcctg
      661 ggcagaaact gaaaattcag accgaggcaa aatcctccgt gcagaaggaa aaatggacag
      721 tcaatctgga ccttttcggc aacagattca ttgaagatga aagccagaac cgtctttact
      781 atataagtac acaggattat tttgcctgca tcgactacat aggaagaaga ggatcgaacc
      841 tgtttatgat gtatctttct ctttacagaa ttcctttcct gtcaatggaa acttcatgga
      901 gagatacaat ctcgtatact catttttcca atccgttagg agtattcttc aaggatctga
      961 tttctccttt ccattcggga atcagctacg actggttcgg cagcataaaa aaagaaggtc
     1021 agtccgaatt tattactgca gaagtaatca agtcgggaat caaaatattt gaatcgcagt
     1081 gcattccggg aaattctgcc tttccgggaa agatcaaggt ttctttcgga gggaaagaat
     1141 gcagtctgtc catcacagaa taaaaaaact tacagtatat attcttatat ttctgatcac
     1201 tctcagtgca tctgccaaga cagtagagga agcatatgca cttatcgaaa agggaaatcc
     1261 agacaaggca gtagatattc tgcagaagat cattcagaaa aatcccaagg actatgctgc
     1321 tcatctgacg cttggttatg ctcatatgct caaagagcgg tatgccaaag ctctggaaag
     1381 ctacagaaaa gcagatgaag ccaattccac tctcgactct ctttcgggaa ttcagtgggc
     1441 ttatctcgct ttgggtcagc atgacgatag cattaaaata ggagaaaaaa tactcgaaaa
     1501 agatcccaaa aatttctatg ccaggtacag gactgcagag tcccttcttg cacagaaaaa
     1561 atacgattcg gcggctaaat attacagatc tctggattca gatttcccca aaaatcagga
     1621 tgtcctgtgg aaactgggcc tctgcgagct ttatcaggga agaatggatg catctgccga
     1681 ttattttcag aagatgcttc aggaaaaccc gaacagttac tctgcacatc tgaacctcgg
     1741 atacatcaat ctgatgaagg aaagaccggc aaaggcgctt gaacattaca gaaaggcgga
     1801 tgaaatttac tccggtctgg attcacagtc aggaatacag tggtctcttc ttgctctggg
     1861 aaaacatgat gaaagcatta gaatgggaga aaaaatactc gaaaaagatc ccaaaaacta
     1921 ttttgcaaga tacaggatcg cagaatccag gctcgcccag aaagactatg aaaatgcagg
     1981 aaagcagtat aaaagtctgc aggcagacga cccaaagaat acagacattc tctggaagct
     2041 cggactctgc gaatactatc agggaaatac atcccgcgca aactcctact ttgaagaagg
     2101 tctgaaaaat gatcctaagc acaaaggtct gagatactct ctcggacttc cgacaacacc
     2161 ctctgttgaa gtcagtcctg aatacacgac atat
//
LOCUS       DAOQGJ010000557         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33183_length_2193_cov_2.950421, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000557 DAOQGJ010000000
VERSION     DAOQGJ010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33183_length_2193_cov_2.950421"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PK683_09505"
     CDS             <1..1087
                     /locus_tag="PK683_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNH08726.1"
                     /translation="LSDKAFRDLDETGVIRIGAEVKAGDILVGMVTPKGETDLTPEYK
                     LLHSIFGEKAKEVRDSSLRMPNGSEGTVIDIKRFSREKGDDLPAGVEEMVKVYVARKR
                     KLVVGDKMAGRHGNKGVVARVVAVEDMPYMEDGTPLDIVLNPLGVPSRMNLGQIFETQ
                     LGFIGEKLGLNFETPVFDGATEADISKYSQEASLPQDSKFALIDGRTGERFINRVFCG
                     YIYMLKLAHLVDDKMHARSTGPYSLVTQQPLGGKARFGGQRLGEMEVWALEAYGASHT
                     LQELLTIKSDDMLGRARIYEAIVKGIHSIKPGIPESFNVLVQELRGLALDIIITDVEG
                     SQVDISDYEDEFSRSKKKIKIESIENI"
     gene            1087..>2193
                     /locus_tag="PK683_09510"
     CDS             1087..>2193
                     /locus_tag="PK683_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004489886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNH08727.1"
                     /translation="MRNYTSFDSITIKIASPERIKDWSYGEVKKAETINYRTLKPERD
                     GLFCEKIFGTTKDWECYCGKFKSIRYKGVVCDKCGVEVTHSKVRRERMGHIELAAPVS
                     HIWYYRSVPSRMGLLLDMTMNALKAILYFEKYVIIDPADSGRNRGELLDEDEYHSYLD
                     EYGDKFLAGIGADAIKELLSRIDVEGEARIIRQRIQEKKVSDKRLIKRLEVLEAFRDS
                     GNRPEWMVLDVVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLALKAP
                     EIIVRNEKRMLQEAVDALFDNSRRKRTVKGKGNRPLKSISDMLKGKQGRFRQNLLGKR
                     VDYSGRSVIVVGPELKFHQMGLPKKMALELFKPFI"
BASE COUNT          652 a          434 c          557 g          550 t
ORIGIN      
        1 tctttcggac aaggcattca gagatctcga tgaaacaggc gtgatcagaa ttggtgcaga
       61 ggtcaaagca ggtgacattc ttgtcggtat ggtgactcct aaaggagaaa ctgatctgac
      121 tcctgaatac aaactgctcc attccatttt cggagaaaag gcaaaagaag taagggactc
      181 ctcactgaga atgccgaacg gatccgaagg aacagtcatt gacatcaaaa gattttccag
      241 agagaaaggt gatgatcttc cagcaggcgt tgaggaaatg gtcaaggtct atgttgcccg
      301 caaaagaaaa ctggttgtgg gggataaaat ggcaggacgt cacggaaaca aaggggttgt
      361 ggcaagagtg gttgctgtgg aggatatgcc ttatatggaa gacggaactc ctctcgacat
      421 cgttctgaat cctctcgggg ttccttcccg tatgaacttg ggtcagatct ttgaaactca
      481 gctcggtttt atcggtgaga agctcggact caattttgaa acaccggtat ttgacggtgc
      541 tacagaagcg gatatttcga aatacagtca ggaagcttct cttcctcagg attccaagtt
      601 tgcattgatt gacggaagaa ctggagaaag atttatcaac agagtgttct gcggatatat
      661 ttatatgctc aaactggctc accttgtgga tgacaaaatg catgcccgtt ccactggtcc
      721 ttactctctt gtcactcagc agcctctcgg cggtaaagct cggttcgggg gtcagagact
      781 cggggaaatg gaggtctggg cactcgaagc ttacggtgca tctcatactc ttcaggaact
      841 ccttaccatt aagtcggatg atatgctcgg aagagcaaga atctacgaag cgattgtgaa
      901 gggaattcat tccatcaaac ctggaatacc ggaatctttc aacgtattgg tgcaggaact
      961 caggggattg gctctggata ttatcatcac ggatgtggaa ggatctcagg tagatatttc
     1021 cgattatgag gatgaatttt ccagaagtaa gaaaaagatc aaaattgaat ccatagagaa
     1081 catctgatga gaaactatac aagctttgac tcaataacca ttaaaattgc ttcacctgaa
     1141 aggatcaaag actggtccta cggtgaggta aagaaagcag agacgatcaa ttaccgtaca
     1201 ttaaagccgg aaagagacgg tctgttctgc gaaaagattt tcggtactac aaaagactgg
     1261 gaatgctact gcggcaagtt taagtccatc agatacaagg gcgttgtgtg tgacaagtgc
     1321 ggagtggagg taactcactc caaagtgcgc agagaaagaa tggggcatat tgaactcgct
     1381 gctcctgttt ctcatatctg gtactacaga tcagttccat ccagaatggg tcttcttctg
     1441 gatatgacaa tgaatgccct caaagcaatt ctgtactttg aaaaatacgt aatcattgat
     1501 cctgcggatt caggcagaaa caggggtgag cttctggacg aggatgaata ccattcttat
     1561 ctggatgaat acggcgacaa gtttctcgct ggaatcggtg cggatgcaat caaggaactt
     1621 ctttccagaa ttgacgtgga aggtgaggca agaatcatcc gtcagagaat tcaggaaaag
     1681 aaagtttccg acaagaggct gatcaaaagg ctggaagttc tcgaagcatt ccgtgattca
     1741 ggaaaccgtc ctgagtggat ggttctcgat gtagttcctg tcatccctcc tgaacttcgt
     1801 ccaatggtgc agctcgacgg cggtcgtttt gcgacgtcag atctgaacga tctgtacaga
     1861 agagtcatca acagaaacaa ccgtctgaaa agactcttgg cattgaaagc gcctgaaatc
     1921 attgttcgta atgaaaaaag aatgcttcag gaagcagttg atgctctttt tgacaattcc
     1981 agaagaaaaa gaactgtaaa aggaaaggga aacagaccgc tcaagtccat ttcagatatg
     2041 ctgaaaggaa agcagggacg ttttcgtcag aaccttctcg gtaagagggt ggactattcc
     2101 ggaagatctg tcattgttgt cggtcctgaa ctgaagtttc accaaatggg tcttccaaaa
     2161 aagatggctc tggaactgtt caagccgttc att
//
LOCUS       DAOQGJ010000558         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33253_length_2190_cov_2.703513, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000558 DAOQGJ010000000
VERSION     DAOQGJ010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33253_length_2190_cov_2.703513"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..939)
                     /locus_tag="PK683_09515"
     CDS             complement(181..939)
                     /locus_tag="PK683_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNH08728.1"
                     /translation="MMTDWILMNLTNLTAALSKIKDAGRVAVLTGAGISAESGVPTFR
                     GTGGYWKNYKAEELATPEAFQANPELVWEWYSMRREVCAKANPNSGHKVIAEMESRYK
                     EFLLITQNVDGLHRRAGSNKMQEIHGCINDARCTFCNQVFPLPPIELTPKLPACPDCG
                     SLARPHIVWFGESYFPGMLEDCNEFTKFADLIFIIGTSGMISAPMNIALAAIRRGAYS
                     IEINPERSTITPNVSLYIQESAGKALTEIWKHLN"
     gene            1109..2005
                     /locus_tag="PK683_09520"
     CDS             1109..2005
                     /locus_tag="PK683_09520"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNH08729.1"
                     /translation="MNVCIFLILFFSVSFLHCGEKEEKKETAPQTEIISPPVLIKDVK
                     SAYSRMKVIDESGIRNLYFVRDSGEEVLESAIHLYTPEKLQVLYTKKMFSSFLIFKNP
                     KSVLMAGLGGGGIVHFMQFFFPDVSLDTVEIDAEIVKTAKELFYVKEKENSRIYIADF
                     LQYAEKTEKKYDLIFLDAFLKPSDRTDPSGTDKRFKEEAFYGLMKKRLNPKGALVINV
                     NTFAGYEQDILRMKKSFSNVYLFNAKGSGNVIAVCSTDSEKVSIQTMSERGDEIDLLR
                     KPNFSFKKLSLLERTEEILKNK"
BASE COUNT          606 a          459 c          489 g          636 t
ORIGIN      
        1 cccacatcct cacggagaat tctccggagg aatttatttc tgagagggag ataggcttta
       61 ttcgagtcgg gatagggatt gacgcggtgt caattttctg cagtgcatcg cagaaaattg
      121 acagaagcgg aaagcccggt cgtcagcagc ctttcgctga cgaatcccca ttgcgcagat
      181 tcagttaaga tgtttccata tctcagtcag tgcctttcct gcactctcct gaatataaag
      241 tgaaacattc ggtgtaattg tagatcgctc agggttgatt tcgatggaat aggctcctcg
      301 tctgattgcg gcaagggcaa tattcatagg tgcactgatc attcctgatg ttcctatgat
      361 aaaaataagg tcggcgaact tcgtaaattc attacagtct tcaagcattc ctgggaaata
      421 ggattcaccg aaccagacta tatgaggtct cgccaatgag ccgcagtccg gacaggcggg
      481 gagtttcggg gtaagctcaa tgggagggag aggaaatacc tgattgcaga atgtgcatct
      541 cgcatcatta atgcatccgt gaatttcctg catcttattt gatcccgccc tgcggtgcag
      601 tccgtcaaca ttctgagtga taagtaggaa ctccttatat cttgactcca tttctgcgat
      661 cactttatgt cctgaattcg gattcgcctt ggcacagacc tcgcgacgca ttgaatacca
      721 ttcccagaca agttcaggat ttgcctgaaa tgcttccggt gttgccaatt cctctgcctt
      781 atagtttttc cagtagcccc ccgttcctct gaaggtagga actccactct cggcagaaat
      841 gcccgctccg gtcagaacgg cgacccttcc ggcatccttg atttttgaaa gtgctgcagt
      901 cagattcgtc agattcataa gtatccagtc tgtcatcatt atattctgtc attgttaaaa
      961 gacctatcgg cagtcaagga aggctcagac agtgatccaa gtcttctgtt tttgcagcca
     1021 atgtcaaggg gaattgctat gcagaattaa atatattttg cagtgtgcca atatgttcag
     1081 tttattgtcc gatcaaaaaa tgaagccttt gaatgtatgc atttttctca ttctgttttt
     1141 ttccgtaagt tttctgcact gcggcgagaa ggaggaaaaa aaagaaacgg caccccagac
     1201 ggaaatcatt tcacctccag ttcttataaa agatgtaaaa tctgcgtatt caaggatgaa
     1261 agtcattgat gagtcaggga tcaggaacct ttatttcgtc cgggacagcg gagaagaggt
     1321 tctggaatca gcaattcatc tttacacacc ggaaaaactg caggtgctct atacaaaaaa
     1381 aatgttcagt tcttttctga tatttaagaa tcccaaatct gtactcatgg ccggactggg
     1441 gggcggggga atcgtgcact ttatgcagtt tttctttcct gacgtcagtc ttgataccgt
     1501 tgagatcgac gctgaaattg taaaaaccgc gaaagaactt ttttacgtaa aggaaaagga
     1561 aaattcaagg atctacattg ccgattttct tcagtatgca gagaaaactg aaaagaaata
     1621 tgatctgata tttctggatg catttttaaa accgtcggac aggactgatc cttcaggaac
     1681 ggacaaacgt tttaaggagg aagcatttta cggtctgatg aaaaaaagac tgaatccgaa
     1741 aggtgcgctt gtaatcaatg tgaatacatt tgcaggatat gagcaggata ttctaaggat
     1801 gaaaaaaagc ttttcgaatg tatatctttt caatgcaaaa gggtcaggaa atgtaattgc
     1861 tgtctgttct actgattccg aaaaagtcag cattcagact atgtctgaga gaggcgatga
     1921 aattgatctt ttgagaaagc cgaatttttc atttaagaaa ctttcccttc ttgaaaggac
     1981 tgaggaaatt ctgaagaata aataactgcg agcagtcctt cttttcacag caaagtctgc
     2041 ttgtgcgcag actctgctgt actgcagact tcaggcaacc gcggccttca tctgtctgac
     2101 tttcattttt tccagccttt tatctctgtc ggccagttct tcttccgttc ccacatatgg
     2161 aattcttgtt ccgaggatat gatcaaaaag
//
LOCUS       DAOQGJ010000559         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33282_length_2189_cov_2.955014, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000559 DAOQGJ010000000
VERSION     DAOQGJ010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33282_length_2189_cov_2.955014"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PK683_09525"
     CDS             complement(<1..1041)
                     /locus_tag="PK683_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08730.1"
                     /translation="MKIRFFSIILPAILNFVSVPVFSEEKTKPELSKLSPDSVRDIKF
                     RIISEKRTVELEWVQPEEDGEIIIGRSNEPIDSAEKFYFADSLGRFPFSKSKPMNIYL
                     DINLKPGRYYYAVGAVKRIRKRDIQMFPDVNYTAVPAVVVGDSMQKVEIPAEENSDKF
                     VKNLTAFPYDQYVRLTWDAPKNAEAGRIMYHIYRSDEPMSSVEELRRARKIIELYHPE
                     TTYVDKTAGLYQEHYYGVAVSSGDEEFVPLIYKESFVKIGNEKLHENPPEAKKETKSE
                     TKSDRKNSEEIPAKTETLQKPEEKPAVPSVIPPAAVHTEEPYFLVKDLSFELKADSYV
                     FTWSPAPGAVPNL"
     gene            1164..1811
                     /locus_tag="PK683_09530"
     CDS             1164..1811
                     /locus_tag="PK683_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH08731.1"
                     /translation="MSIYTPDMEYFEDIKYQEFLLTSKRKAICPPEKIFQEIRYRWKD
                     ARNIVDFGMGLGFFTPFIRQRMHKDAWLWGVECQQDLIDLILKKKVDQNITNFSAVFL
                     EKTEHPLLPQWIPHPDLIFASLSLSTFANPGLAMDGLVRSMNDGGTLVIIDWAKTDHP
                     SGPQLKDKVSAEKMKYLAELYDLEVVNYFSITEFFYGLEVKPSPDFKFKFYDYRD"
     gene            1804..2088
                     /locus_tag="PK683_09535"
     CDS             1804..2088
                     /locus_tag="PK683_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08732.1"
                     /translation="MTDAEGSFSFIALYCSDIYISKKFYEETFSLIFNEEKHGEGPMH
                     FCASLGQSVLELYPAGKWNSSGISFGLKLSGSGRIQRFTDPDGNTVFAEI"
BASE COUNT          616 a          441 c          455 g          677 t
ORIGIN      
        1 aagattgggc actgcacccg gagcaggcga ccaggtaaaa acgtatgagt ctgccttgag
       61 ttcaaaactc agatccttta caagaaagta gggttcctca gtatgaacgg cggcaggagg
      121 aatgactgaa ggaacagcag gcttttcttc aggtttctgt aaagtctctg tctttgcagg
      181 gatttcctca gagttttttc tgtcagactt ggtttcagac ttcgtttcct tttttgcttc
      241 cggaggattc tcatgaagtt tttcatttcc gattttcaca aaggattctt tgtaaatcag
      301 ggggacgaac tcttcatctc cagaggaaac ggccaccccg taataatgct cctgatacag
      361 tccggccgtt ttatccacat aggtggtttc aggatgatac agttcaatga ttttccttgc
      421 tctcctgagt tcttccactg aagacatagg ttcatctgat ctgtatatat gatacatgat
      481 tcttcctgct tctgcatttt taggagcatc ccaggtcaga cggacatact gatcgtaggg
      541 aaatgcagta agattcttta caaatttgtc actgttttct tcagcaggaa tttccacttt
      601 ctgcattgag tcacccacta caactgccgg aacagcagta taattgacat caggaaacat
      661 ctgtatgtcc cttttcctga tccgttttac cgctccgact gcatagtaat aacgtcccgg
      721 tttcagattt atgtccagat aaatattcat tggcttcgat ttggaaaaag gaaatcttcc
      781 gagagaatca gcaaaataaa atttttctgc agaatctatc ggttcattgg atcttcctat
      841 aatgatttca ccgtcttctt cgggctgaac ccattccagt tccactgttc ttttttctga
      901 aataattctg aactttatgt cccgaactga gtcgggagac agtttggaga gttctggttt
      961 tgttttttcc tctgaaaata caggaactga gacgaaattt aaaatggcag gaaggataat
     1021 ggaaaaaaat ctgattttca tactgaatta gaccatttta actaatgtaa gtatcgacaa
     1081 aatcagcctg taaatccaga ttgtacgata aaaactaaag ttgtcggtca tttccgaacg
     1141 actcagttag gaagggaaat tcaatgagta tttatactcc tgatatggaa tactttgaag
     1201 acatcaagta tcaggaattc ctgctgacaa gcaaaagaaa ggcaatttgc ccgcctgaaa
     1261 aaatttttca ggaaatccga taccgatgga aggatgcgag aaatatcgta gattttggaa
     1321 tgggtctggg atttttcacg ccttttatcc gtcagaggat gcataaggat gcctggcttt
     1381 ggggagtgga atgtcagcag gacctgattg atctgattct taagaaaaaa gtagatcaga
     1441 atattacgaa tttttctgcc gtttttctcg aaaagacaga acatcctctt ctgcctcagt
     1501 ggattccgca tcctgatctc attttcgcat ctctcagtct ttccacattt gccaatcctg
     1561 gtcttgcgat ggacgggctt gtccgatcca tgaatgacgg aggcacactg gtcatcatag
     1621 actgggcaaa gacagatcat ccgagcggtc ctcagctgaa agataaagtt tctgcagaaa
     1681 aaatgaaata tcttgcagaa ctctatgatc ttgaggttgt caattatttt tccattacgg
     1741 aattctttta cggtcttgaa gtgaaaccca gtcctgattt caaatttaag ttttacgatt
     1801 accgtgactg atgctgaagg ctcattctcc tttattgctc tttactgcag tgatatttat
     1861 atctcaaaaa aattctatga agaaacattt tcattaattt ttaatgaaga aaagcacggt
     1921 gagggtccga tgcatttctg cgcttctctt gggcagtcgg ttcttgagct ttatcctgcg
     1981 ggaaaatgga acagctcagg aatttcattc ggactgaaac tttccggttc cggcagaatt
     2041 cagcgtttta cagatcctga cggaaatact gtttttgcag aaatctgatt ttttttctgc
     2101 agataaacag aaaacattgg aaccttatct gaaaaacagg gtcacactga caaattcgga
     2161 agaacccgta gaaccgggaa cttccaagg
//
LOCUS       DAOQGJ010000560         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33395_length_2185_cov_3.331455, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000560 DAOQGJ010000000
VERSION     DAOQGJ010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33395_length_2185_cov_3.331455"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PK683_09540"
     CDS             <1..909
                     /locus_tag="PK683_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNH08733.1"
                     /translation="LMLFFEKKPEYKVCFAAAGGGCRAFYAMGFGYALRTFGVRFEGM
                     SGVSAGSAMILGILSETEEKSVEYIASLTKRNEKNFSLRRLLKGDRPFPHENIYRRTI
                     RYGMDFNKITGSNVKVYIQAVLALLKQEGITDLINKAKIIAKTGQAYLLDEADRMKGL
                     PGNRVEKLAKEWNFREVIYTNEHLKDPEIMEQIILNSSSIPPIVTFQNVENEYYFDGG
                     LFNNLLLEVFPLESRKIGIYYDDTSVFGKSEEVLKNTLLFKPSRPLRITSFDYTNPEG
                     LIDTYELGKYDAMKNKEKVMEFCRID"
     gene            902..1660
                     /locus_tag="PK683_09545"
     CDS             902..1660
                     /locus_tag="PK683_09545"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNH08734.1"
                     /translation="MTKLSVNVNKIATPRNSRGGNIPDLMEYSKIIMDAGAHGITVHP
                     REDERHIRRTDVFHLKKLIKEYNGINNSKIEYNIEGEPSQRFRDLVLEAAPDQVTLVP
                     VTPGEITSDHGFQLKTEGEFLSEYISIFKKQGIRVSIFMETDAENIRLAKNTGTDRIE
                     LYTGPFAENYSEGKEKESFEAYRTAAEAALSSGLGINAGHDLDTENLKLFRKLPGLLE
                     VSIGHRLMSRALLTGLKTAVREYLEILSEDVSHS"
     gene            complement(1614..>2185)
                     /locus_tag="PK683_09550"
     CDS             complement(1614..>2185)
                     /locus_tag="PK683_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08735.1"
                     /translation="ISAKFPETGGAELFAEVQNLCKSNDRIKLLAVLDEISSKGLLSS
                     NSFTTGLLGDCLSNEEKALYIKKNSPNKADFASLYYKEKAKVHPFSQFWDTEYFVQKK
                     KLEDYKADIKSPLTREWAAFRKSVRENNVRGAEANLRSFLSVLKTEKDKKKSDKSYQA
                     VYRAAYLLVEQDEFFRNVRHLLKEFPDTL"
BASE COUNT          674 a          418 c          501 g          592 t
ORIGIN      
        1 cttatgctgt ttttcgaaaa aaaacctgaa tacaaagtgt gtttcgctgc agccggggga
       61 ggttgcaggg cattttatgc aatgggtttc ggatatgcac tgagaacctt cggagtacgg
      121 tttgagggaa tgtcaggggt gagtgccggt tccgcaatga ttctgggaat tctatctgaa
      181 acggaagaga agtcagtgga gtacattgca tctcttacaa aaagaaatga aaagaatttc
      241 agtctgagga gactgctcaa gggagacaga ccctttcctc atgaaaatat ataccgcagg
      301 acaattcggt acgggatgga tttcaataaa attacaggaa gcaatgtaaa ggtttatatt
      361 caggctgtgc ttgcactgct caagcaggag ggaatcactg atctgatcaa caaggccaag
      421 atcattgcaa aaactggcca ggcctatctt ctggatgagg ccgacagaat gaaaggactt
      481 cctggaaacc gggttgagaa actggcaaag gaatggaatt tcagagaagt catttataca
      541 aatgagcatc tgaaagatcc ggaaattatg gaacagatta tactcaactc atcgagcatt
      601 ccgccgatag tcacgtttca gaatgtggaa aatgaatact attttgacgg aggccttttc
      661 aacaatctcc ttctggaagt atttcctctt gaatcgagga aaataggaat ttattacgat
      721 gatacatctg tcttcggcaa gtctgaagaa gttctgaaaa atactctgct tttcaagccg
      781 tcgagacctc ttcggatcac ttcatttgac tacaccaatc cggaaggact gatcgacacg
      841 tatgaactgg gcaaatacga tgcaatgaaa aataaggaaa aagtaatgga gttctgcaga
      901 attgactaaa ctcagcgtaa acgtaaacaa gatagctact ccccgcaatt ccaggggcgg
      961 aaacattcct gaccttatgg aatattcaaa aatcatcatg gatgcaggtg ctcacgggat
     1021 tactgtccat ccgagggaag atgaaaggca tataagacgg acagatgtat ttcatctcaa
     1081 aaaattgatt aaggaataca acggaatcaa taacagtaaa attgaatata atatagaagg
     1141 tgaacccagt cagagattca gggatctggt tctcgaagca gcaccggatc aggtgactct
     1201 tgttccggtg actccgggcg agattacatc cgatcatggg tttcagctga agacggaagg
     1261 agaatttctt tcagaatata tcagcatttt taaaaaacag ggaatcaggg tatccatatt
     1321 tatggaaact gatgctgaga atatccgtct cgcgaaaaat acaggcactg acagaataga
     1381 actgtacact ggaccgtttg cggaaaatta ttctgaagga aaggaaaagg aaagctttga
     1441 agcatacagg actgctgcgg aagcagctct ttcttcagga ttagggatca atgcaggaca
     1501 tgatctggac actgaaaatc tgaaactgtt cagaaaactt ccaggacttc tggaagtttc
     1561 catcggtcac agactgatgt ccagggcact tttaacagga ttgaaaactg cagtcagaga
     1621 gtatctggaa attctttcag aagatgtctc acattcctga agaattcgtc ctgttccacc
     1681 agtaaatacg ctgctctgta gactgcctga taggatttat ctgatttctt tttgtctttt
     1741 tccgttttca gaacggaaag gaaagatctc agattggcct ctgctcctct gacattattt
     1801 tctctgacag attttctgaa ggcggcccat tcccgtgtaa gaggggactt gatatcggcc
     1861 ttgtaatcct cgagcttttt tttctgaaca aagtattctg tatcccagaa ttgagaaaaa
     1921 ggatgaacct tagccttctc cttatagtag agagacgcaa aatctgcctt gttcggtgaa
     1981 ttctttttga tatacagtgc tttttcttcg ttcgaaagac agtctcctaa aagtccagtt
     2041 gtaaatgaat ttgaactgag cagacctttt gaactgattt cgtccagaac cgcaagaagt
     2101 ttgattctgt cattggattt gcagagattc tgcacttctg caaaaagttc tgcgccgcca
     2161 gtttcaggaa attttgcact gatgt
//
LOCUS       DAOQGJ010000561         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33416_length_2184_cov_3.623767, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000561 DAOQGJ010000000
VERSION     DAOQGJ010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33416_length_2184_cov_3.623767"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PK683_09555"
     CDS             complement(<1..755)
                     /locus_tag="PK683_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08736.1"
                     /translation="MKFAWMVMIILFLGCETEKAEMNKANTRAGLIFTALNSGASAVN
                     QNSGGTVSVVLSKSALSFDEDRRQDLNMNTVQPLELQVSSATSALIATDGSEFILTVY
                     SPAGVKLTSYQMAIQRSDYLSGKGTFFSFSETGTYRIEVENNIKSGSFYLSVRTKPVS
                     SAAKNYTGVYNTKKVCIETLGQPTSGFTESAGPCPSAGRIAQTSAYLRCSSFIGTKSC
                     EGANDTRGSFSPWAYHSRSYYSADHTLDSLTSL"
     gene            complement(763..1194)
                     /locus_tag="PK683_09560"
     CDS             complement(763..1194)
                     /locus_tag="PK683_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08737.1"
                     /translation="MGIVKAKAVLESGITASNTFDSSGRLHLAEYSRTSSAGDFRYKN
                     EKREFGSYDEFGRPASSTYTFYFEGDIFYGFSPYYGSYPERYSYDDANRKMRSEIQNY
                     SSAKTLTRSFNQDNILSYEYAGTDSSKGTYYDIKSTLRVCY"
     gene            complement(1493..>2184)
                     /locus_tag="PK683_09565"
     CDS             complement(1493..>2184)
                     /locus_tag="PK683_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08738.1"
                     /translation="PQWNQGTQTAFFKSRPFPWIFYPDSLDLSSIKFVSDYPYYSDKA
                     KLVELIIDSLMNVELEVAIRIQPDGTIQYSWRWTSLGNFDWLSYATDSIWFALDRTMS
                     VNGRLEYYYLSTSSFQAVYNSAGLGLTADSLLNAKKYAACFFDSVRPDFGLGFHYGDI
                     QTQGGASISLWPGASNDSGSVQIVATNMFPAFSYPGNLFRQNFFNFGYLAEIDSKYEL
                     LKNYLAFLSNT"
BASE COUNT          695 a          453 c          403 g          633 t
ORIGIN      
        1 cagagagaag taagtgagtc caaagtatga tccgcagaat aataggatct gctgtggtat
       61 gcccagggag aaaatgaacc tcttgtatca ttggcacctt cacagctttt tgttcctata
      121 aatgaggaac agcggagata ggcagaggtc tgcgcaattc ttcctgcact tggacagggt
      181 cccgcacttt cagtgaatcc gctggtcggc tgtcctaatg tctcaataca tactttttta
      241 gtattgtaaa cacctgtata atttttcgcc gcagaactta caggttttgt tctgacagac
      301 agataaaaag agccggattt tatattattc tctacttcaa ttctgtatgt gcctgtttca
      361 gaaaaagaaa agaaagttcc tttgccggaa agatagtcgg atcgctgaat cgccatctga
      421 taagaagtca gttttacacc tgcaggagaa tacacagtca gaataaactc agaaccgtca
      481 gtggcaatca aagcagatgt cgcagaactc acctgaagct ccaaaggctg cactgtattc
      541 atatttaagt cctgtcttct gtcttcatca aaagataaag cagatttgga aagcacaaca
      601 gaaacagtac ctcccgaatt ttggtttact gcagatgcac cggaattcaa agctgtgaaa
      661 atcaggccag ctctggtatt tgccttgttc atttcagctt tttccgtttc acatcccaga
      721 aaaagaataa tcattaccat ccatgcaaat ttcatgattt tttcagtaac acactctgag
      781 agtcgattta atatcatagt aagttccctt agaactgtct gtgcccgcat attcataaga
      841 aagaatatta tcctgattaa aagatctggt cagagttttt gcagaagaat aattctgaat
      901 ttccgatctc atctttctgt ttgcatcatc ataagaatac ctctcaggat aactgccata
      961 ataaggagag aaaccgtaaa aaatatcgcc ttcaaaataa aaagtatatg ttgaactcgc
     1021 tggtctgccg aattcatcat aactcccgaa ttcccttttt tcattcttat atctgaaatc
     1081 acctgcactg gatgtcctgc tgtattcagc aagatgcagt cttcctgagg aatcaaatgt
     1141 gttcgaggca gttatcccgc tttccagaac tgctttagcc tttactattc ccaaagtctt
     1201 ctgttccaag acaaaatcct tcgcagaatt ataagttcgt tgagaaacca aagcacttga
     1261 agtgtttctg cagttcaaag tattattaga gaactcacaa ttgaatgcct gagcattcgg
     1321 agcatcgcag ttggaaatac agtaaacgct tgcatacgct gcacagttct gtcgagaaaa
     1381 agtaagcagc gcggcagaca gcagaaactt tctgtcgttg ctttctttgt tttggggatt
     1441 gcagaaagaa aaagcaagca ataaaattac taattttttc atttctgtac cctcaggtat
     1501 ttgaaagaaa tgcaagataa ttctttaaca gctcatactt actgtctatt tcagctaaat
     1561 atccaaaatt gaagaaattc tgcctaaata aatttcctgg ataagagaag gcaggaaaca
     1621 tatttgtggc tactatctga acggacccag aatcatttga agcacccggc caaagtgaaa
     1681 tggaagcacc tccctgagtt tgaatatctc cataatgaaa acctaatcca aaatcaggcc
     1741 ttactgaatc aaagaaacag gctgcatatt tctttgcatt caaaagacta tctgcagtca
     1801 atcctagacc ggcactgtta tatactgctt gaaatgaact agtcgataga taatagtatt
     1861 caagtcttcc attgacagac atagttcgat ccaatgcaaa ccagatagaa tctgttgcat
     1921 aactcaacca atcaaagttt cccaaagaag tccagcgcca agaatactga atagttccat
     1981 ctggttgaat tcgaattgcc acttccagct caacattcat taatgagtca atgattaact
     2041 caacaagttt tgccttatct gaataatatg ggtaatctga cacaaatttt atactgctta
     2101 aatccaacga atcgggataa aaaatccaag ggaatggcct tgatttaaag aaggctgtct
     2161 gtgtaccttg gttccactgg ggat
//
LOCUS       DAOQGJ010000562         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33501_length_2181_cov_2.342427, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000562 DAOQGJ010000000
VERSION     DAOQGJ010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33501_length_2181_cov_2.342427"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2181)
                     /locus_tag="PK683_09570"
     CDS             complement(<1..>2181)
                     /locus_tag="PK683_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HNH08739.1"
                     /translation="QDKNKQAPFIIENASEGEYLSMDKTKSGVLVLRGNVKIKLAEGS
                     LSAETISVDSKKNEIYAEGGIVYKNGPMEVKGDKFIYDIKLDRGIIYETKAALHPTYF
                     VGKKIKKVDENKYLLDMGYFTTCNAEVPHYSFRAKKIFVYEDKSIVATNLSLKVGAAP
                     IFWTPLFFGSNLGSGWMVQFGQNRTQGKFLQTSYQWSDPTAIPSLLMPVGRKIKFDAY
                     QITGYHAGFEFWKVSPHLNYNLDLGVADYHHYQFATRYAGYVPGKFGELDRFLVPGQN
                     EIVTTNQVDRGDSCTRVGAVCVRNQDIFASQGITTSRLPSIGPQNETWWKGNLIANIK
                     SNNVANDGTRNLQIRYENYTNPRFDYEYGFRYDPGNTLQALYTNRTSRNPLFRSNLTW
                     NADYTETRGDLSINVSARRMQTYYFLNNASNGVDRSGFFPVRDELPRTQIRNSSNIAN
                     LPYFNSPVYWDMNLTNLVVRSYGGPVRTPLAGSTSALVIDDPYGKYKTNLLRTEYTTN
                     METGFRTNMNFGSYVTLTPGIYGGMQKKSMERADSTATASTGELSLERFYKRESYKFL
                     RQNHRLSFGVPALLFSTTYRRILASGRELAEPVLKDGKDAQHEVEFALESNAFEDFEM
                     SVRTIRDLRQFSDQYRPAPTSQERWYFTVFRLGTFYDFYDGFSKKRSTLLERQRSFFS
                     GIFLNNDHVYHTAQKRPLYNNASLGYQMGGFSFWFIRNIKSLELG"
BASE COUNT          595 a          486 c          438 g          662 t
ORIGIN      
        1 cccagttcca ggcttttaat atttctgatg aaccagaatg aaaagccgcc catctgataa
       61 ccgagagatg cattgttgta aagaggacgt ttctgagcag tatggtatac atgatcatta
      121 tttaaaaata tgcctgagaa aaaacttctc tgtctttcca gaagtgtgga tctttttttg
      181 ctgaacccgt cataaaaatc ataaaaggtt cccagtctga acacagtgaa ataccatctt
      241 tcctgggatg taggagcagg tctgtactga tcggaaaact gtctgagatc cctgattgtc
      301 cttactgaca tttcgaagtc ctcaaatgca ttgctctcta aggcaaactc aacttcgtgc
      361 tgagcatcct tcccgtcttt cagtaccggt tcagccagct ctctcccgct cgcaaggatt
      421 cttctgtagg tagtggagaa aagaagtgca gggacaccga aactcagtct gtggttctgt
      481 ctgagaaatt tataggattc tcttttataa aacctctcca gggaaagctc acctgtgctc
      541 gccgttgcgg tagaatcagc tctttccatg gactttttct gcatacctcc gtaaattcca
      601 ggagtaagag tcacataact tccgaaattc atatttgtcc taaagccagt ttccatattc
      661 gtggtatatt cagttctcag aaggtttgtc ttgtattttc cataaggatc atcaatgaca
      721 agagctgaag tggaaccggc cagtggtgtt ctgacaggcc ccccgtagga gcggacgaca
      781 agatttgtca gattcatatc ccaatagacc ggggaattaa aataaggaag gttggcaatg
      841 tttgaagaat ttcttatctg agtacgagga agttcatccc gaaccggaaa aaaacctgat
      901 ctgtctacac cgtttgaagc attgttcaga aaataataag tctgcattct tcgcgcactt
      961 acattgatgg aaaggtctcc tcttgtctca gtataatctg cattccaagt aagattggat
     1021 ctgaatagag gatttctgga ggttctgtta gtatacaatg cctgaagagt attgcctgga
     1081 tcgtaacgga acccgtattc ataatcaaat ctgggattcg tatagttctc atatctgatc
     1141 tgaaggttcc ttgtaccgtc attggcaaca ttgttagatt ttatgtttgc aatcagattc
     1201 cccttccacc aagtctcatt ttgcggtccg atactgggaa gtctcgaagt tgtaattccc
     1261 tgagatgcaa aaatgtcctg atttctgaca cacaccgcac cgactctggt acaggaatca
     1321 cctctgtcta cctggttcgt tgtaacaatt tcattctgtc ccggaacaag aaatcggtca
     1381 agttcaccga atttgcccgg aacatatcct gcatatcggg tggcaaactg ataatgatga
     1441 taatcagcca cgcccagatc cagattgtaa ttcagatgag gactgacctt ccagaattca
     1501 aacccggcat gatatcctgt tatctggtaa gcatcaaatt tgatttttct tcctactggc
     1561 ataagaagag aaggaattgc agtcggatca gaccattgat atgatgtctg gaggaatttc
     1621 ccctgggttc tgttctgacc gaactgcacc atccatccgc ttccgagatt ggaaccgaaa
     1681 aacagaggcg tccagaaaat aggcgctgca cctactttca gtgaaaggtt tgtagcaaca
     1741 atgcttttgt cttcatatac aaaaatcttc tttgcgcgaa agctgtaatg aggcacctct
     1801 gcattgcagg tagtgaaata ccccatatcc agaagatatt tattctcatc taccttcttg
     1861 atttttttac ctacaaaata tgtcggatga agggcagctt tggtttcgta tattatccct
     1921 ctgtccagtt ttatatcata aataaactta tcacctttga cttccattgg accattctta
     1981 taaacaatcc cgccttccgc ataaatttca tttttctttg aatccactga aattgtttcc
     2041 gcagaaaggc ttccctcggc cagtttgatt tttacgttcc cgcgaaggac aagaacgcct
     2101 gattttgtct tgtccatcga aagatattct ccttccgaag cattctcaat gatgaacggt
     2161 gcctgtttat ttttatcctg a
//
LOCUS       DAOQGJ010000563         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33515_length_2180_cov_3.572706, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000563 DAOQGJ010000000
VERSION     DAOQGJ010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33515_length_2180_cov_3.572706"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PK683_09575"
     CDS             complement(<1..732)
                     /locus_tag="PK683_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNH08740.1"
                     /translation="MKIFCLSLIFGRILAVTAVFSASLFCSKLPSIPACTDSNPDICR
                     NEARMFIGRLLFYDTALSINDKVSCASCHSQKLSFTDGIGLSTAGVTGVPLKRHSPAL
                     LNLQSVNKGLFWDGGAKNLRSLVFAPITNKDEMGLSLGLLVKKVEDRGYKKLFEKAFS
                     DGEVSIANIALSMESFLLTMVSFNSKYDRVIRGEEKFSEQEKKGYAIFQKNCSSCHPE
                     PDFSDHSFYNVGLDEKIIKDIEKEPD"
     gene            complement(729..>2180)
                     /locus_tag="PK683_09580"
     CDS             complement(729..>2180)
                     /locus_tag="PK683_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08741.1"
                     /translation="SIFGFSVSTHLFPVFKADSGNTVTITDMKYWLSNLKFIKADGTE
                     VSIQDSYYFMTDNNLIPADGDHFNAYPARKREDVDIWIPYGEYKGVKYSIGVDSVYNG
                     DLGKHAGELSETSRGLNVNWMWFSSYIFTRVQGTCTSCTGTKPFKLDTGADSSYRTVT
                     HNFQSNITVSKDNSSPKFVLKMDVKKLFTGIDIQTLATSGGKRIIGATTPSDMTKLAD
                     NTSGSTSLVSASSDSLAEANRVLVTLEMSKFFDSSEFKLGTQYTNSAGNQLTFSSFRY
                     YLSNVNFIKADGTAVPVYKSYYAMEMNNAETADGANIPSWVTWEAKNREKVFVYIPAD
                     SYTSIQFSIGVDPTYNDDMTKQAGDLRLYSNLTQDNGWMWFTSYIFSRMKGTCSNCAS
                     PQSFTLDTGSNSSYRTVTVPFGKTVTTAYDKDVKVKLKLDISKLFTSLNVGSLPGGSG
                     DRIISATTPTEMTTLSNNYSSAFSLIEVTSAGR"
BASE COUNT          571 a          472 c          467 g          670 t
ORIGIN      
        1 gtcaggttct ttttcgatat ccttaatgat tttttcatcc agccctacgt tgtaaaaact
       61 atgatcgctg aaatccggtt ccggatgaca ggaagagcag tttttttgaa atatagcata
      121 tcctttcttt tcctgttcgg agaatttttc ttcccctctg attaccctgt catatttgga
      181 attgaaggag accatagtca gaagaaaact ttccattgag agagcgatat ttgcgatgga
      241 gacttccccg tctgaaaaag ctttttcaaa cagtttttta tatcccctgt cttccacttt
      301 cttgactaaa agtcccagac tgagtcccat ctcatccttg tttgtgatcg gagcaaagac
      361 aagggaacgc aggttttttg caccgccgtc ccagaagagc cccttgttca ctgactgaag
      421 gttgagcagg gcaggggagt gacgtttcag cggaactccg gtaacgcctg cggtgctgag
      481 tccaattccg tctgtgaatg aaagcttctg actgtgacag gatgcacagg atactttatc
      541 attaatcgag agtgcagtat catagaaaag cagtctgcct ataaacattc ttgcctcatt
      601 tctgcagata tccgggtttg aatcagtaca cgcaggaatg gacgggagct ttgaacagaa
      661 cagtgaggct gaaaaaacag ccgtcactgc aagtattctt ccgaagatca gagatagaca
      721 gaaaatcttc atctgcctgc actggtcact tcaatcagtg aaaaagcgga agaataatta
      781 ttggaaagag ttgtcatctc tgtcggtgtc gttgcactga tgatcctgtc cccgcttcct
      841 cccggaagtg aacccacatt cagagaagtg aaaagttttg aaatatccag tttcagtttc
      901 accttcacat ctttgtcata tgcagtcgtc actgtttttc cgaaaggtac agtgactgtt
      961 ctgtaacttg agttggagcc tgtgtcaaga gtgaatgact gaggacttgc acagtttgaa
     1021 caggttccct tcatcctcga aaagatatat gaagtaaacc acatccagcc gttgtcctgt
     1081 gtcaaattgc tgtagagcct caagtcaccg gcctgtttag tcatatcgtc attgtaagtc
     1141 ggatcgacac cgatggaaaa ctgaatgctt gtataactgt ctgcaggaat atagacgaaa
     1201 accttttccc tgttttttgc ttcccaggtt acccatgagg gtatatttgc cccgtcagca
     1261 gtttctgcat tgttcatttc cattgcataa taggatttgt atacaggcac tgctgttcca
     1321 tcagccttga tgaagttcac attgctcaga tagtatcgga atgaactgaa ggtcagctga
     1381 tttcctgcag aatttgtgta ctgagttccc agtttaaact cagaagaatc aaagaatttg
     1441 gacatttcca gggtgaccag tactctgttt gcttcagcga gactgtcgct ggatgccgat
     1501 acgagtgaag tgcttccaga tgtgttgtca gccagttttg tcatatctga aggagttgtt
     1561 gcacctatga tcctttttcc tcctgatgtt gcaagcgtct gaatgtctat tcctgtaaaa
     1621 agctttttaa catccatttt taagacgaat tttggggaac tgttatcctt gctgactgta
     1681 atatttgact gaaaattatg cgttacagtt ctgtagcttg aatcagctcc tgtatccagc
     1741 ttaaaaggtt ttgttccagt gcatgaggtg caggttcctt gtactctcgt aaaaatataa
     1801 cttgaaaacc acatccagtt tacattgagt cccctgctgg tttcgctcag ttctccagcg
     1861 tgtttcccca gatctccgtt ataaacagaa tctactccaa tcgaatactt gactcctttg
     1921 tattcgccgt atggtatcca tatatccaca tcttctcttt tccttgcagg ataagcatta
     1981 aaatggtctc cgtctgcggg gatcagattg ttgtcagtca taaaataata ggaatcctga
     2041 atgctgactt cagttccgtc tgccttgata aatttcagat tggaaagcca gtacttcata
     2101 tctgtgattg taaccgtgtt cccggaatca gccttgaaga caggaaacaa atgggtggat
     2161 acggaaaacc cgaatatgga
//
LOCUS       DAOQGJ010000564         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33528_length_2180_cov_2.302588, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000564 DAOQGJ010000000
VERSION     DAOQGJ010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33528_length_2180_cov_2.302588"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PK683_09585"
     CDS             <1..568
                     /locus_tag="PK683_09585"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNH08742.1"
                     /translation="VLEDGRVLVSNDHGSGGKFRNFMDDLFRFKRSEVVLYDGQNWKT
                     LEDTGASFGNGIIVLKKNDKAVMYRASTIKEKIDRFEITDWKTFELKKMDPIKMESGP
                     DNLETDEEGNLWTAAHISMFRFLFHASDPKNHSPSQVFKISPDGVITEIYADKGEEIS
                     ASSTGLKIGKRLYIAQVFEDYLLSCPME"
     gene            613..1266
                     /locus_tag="PK683_09590"
     CDS             613..1266
                     /locus_tag="PK683_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNH08743.1"
                     /translation="MELLKLAIDFVLHIDRYLDQITREYGLLTYVILFLIIFAETGLV
                     VTPFLPGDSLLFAIGAICALGSLNIYLIIGLLIIAAILGDTVNYAIGHLMKDRFLAGE
                     KIKFIKQEHLNKTHEFYEKYGGKTIIIGRFIPIIRTFAPFVAGIGAMEYKKFILYNVI
                     GGIVWVLIFTVAGYIFGGAEIIRKNFTLVIFAIILISVMPAVIEYIRHKNSPKGDAK"
     gene            1263..>2180
                     /locus_tag="PK683_09595"
     CDS             1263..>2180
                     /locus_tag="PK683_09595"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNH08744.1"
                     /translation="MRRNIVHPGADELIYEIRQIVGVGKHLEKLGVKITWENIGDPVQ
                     KGEKIAPFIKKIVSDLVQNDLSWAYTATQGYEKTRNFLAEKVNERGGTQITPDDIVFF
                     NGLGDAVAKIFGFMRREARILGPSPAYSTYSSAEAAHSGYEHLTYELNPDNGWMPDIN
                     DIENKVRYNDSIAGILLINPDNPTGAVYDREIIKKIVQICEKYNCILICDETYAHVNY
                     SGSGSIHLSECIGDKACGMALRSISKELPWPGSRCGWIEVFNRKNDPLFDRYIKSLVD
                     AKMLERYLHTKYVGQKRFTFTSYLFRNRLR"
BASE COUNT          669 a          463 c          487 g          561 t
ORIGIN      
        1 tgttcttgag gatggaagag ttcttgtatc aaacgatcac gggagcggcg gcaagttcag
       61 aaattttatg gatgaccttt tccgtttcaa aagatcggag gttgtactgt acgacggtca
      121 gaactggaag acactggaag atacaggagc ctctttcgga aacggaatca ttgttctgaa
      181 aaaaaatgac aaggcggtca tgtacagggc atccacgatc aaggaaaaaa ttgacagatt
      241 tgaaatcact gactggaaga cttttgaact gaaaaaaatg gatccgatca aaatggagtc
      301 agggcccgac aatctggaaa cagatgaaga aggaaatctc tggactgcag cccatatttc
      361 catgttccgt ttcctgttcc atgcatccga tccgaaaaat cattcccctt cacaggtatt
      421 caaaatttca ccggacggag taatcacaga aatctacgca gacaagggcg aagaaatctc
      481 tgcatcgagc accggtctga aaatagggaa aagactgtac attgctcagg tattcgaaga
      541 ctatcttctc agctgtccaa tggaataata tcgcttttca aagtcaaagc ttttcaaaaa
      601 ctggaaatta tcatggaact tctgaaatta gcgatagatt ttgtgctcca tatcgacaga
      661 tatctggacc aaattacaag agagtacggt ctgctgacct acgtcattct gtttctcatc
      721 atttttgcgg aaactggtct tgtcgtcaca ccctttctgc cgggtgacag cctccttttt
      781 gcgatcggag ccatctgcgc cctgggttca ctcaacatat atttgattat aggtctgctt
      841 attatcgctg caattctcgg agatactgta aactacgcaa tcgggcattt aatgaaggac
      901 agatttcttg caggtgaaaa aatcaaattc atcaagcagg aacatctgaa caaaactcac
      961 gagttttacg agaaatacgg cggcaagaca atcatcatcg gaagattcat tccgatcatc
     1021 agaacatttg cgcctttcgt tgcaggcatc ggcgcgatgg aatacaaaaa attcattctg
     1081 tacaatgtga tcggcgggat cgtatgggtt cttatattca ctgtggcagg atatatcttc
     1141 ggcggagcag aaattattcg gaagaatttc actcttgtga tttttgcaat catactgatt
     1201 tctgttatgc ctgcagtcat tgaatacatc agacataaaa attcacccaa gggagatgca
     1261 aaatgagaag aaatattgta catcccggcg ctgatgaact gatttatgaa atccgtcaaa
     1321 ttgtaggggt aggaaagcac ctggaaaaac tgggtgtaaa aatcacctgg gaaaacatcg
     1381 gtgatcctgt ccagaaagga gagaagattg cacctttcat caagaagatt gtatctgatc
     1441 ttgttcagaa tgatctttcc tgggcttaca ctgcgacaca aggttatgaa aaaaccagaa
     1501 attttcttgc tgaaaaagtg aatgaaagag gcgggaccca gatcactcct gacgatattg
     1561 tattcttcaa cggactggga gatgctgttg cgaaaatttt cggctttatg agaagagaag
     1621 ccagaattct gggaccaagc cctgcctact ccacctactc ttctgctgag gccgctcaca
     1681 gcggttacga acatctgact tatgagctga atccggacaa cggctggatg ccggacataa
     1741 atgatataga aaataaagta cgttataatg attccatcgc aggaatactc ctcattaatc
     1801 cggacaatcc tacaggagca gtttacgaca gggaaataat caaaaaaata gtgcagatct
     1861 gcgaaaagta caactgcata ctgatctgcg acgaaacata tgcccatgta aactattccg
     1921 gaagcggctc cattcatctt tctgaatgca taggcgacaa ggcctgcgga atggcgcttc
     1981 gttcgatctc gaaggaactc ccctggccgg gttcccgatg cggctggata gaagtgttca
     2041 acagaaaaaa tgatcctctg ttcgacagat acatcaagtc tcttgtagat gcaaaaatgc
     2101 tggaacgcta cctgcatact aagtacgtcg gtcagaagcg cttcaccttc acgagttatc
     2161 ttttccgaaa ccggctgcgc
//
LOCUS       DAOQGJ010000565         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33531_length_2180_cov_2.068706, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000565 DAOQGJ010000000
VERSION     DAOQGJ010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33531_length_2180_cov_2.068706"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PK683_09600"
     CDS             <1..736
                     /locus_tag="PK683_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HNH08745.1"
                     /translation="VIGGKVYIGTEGRVWIFHSQGTTGIASNTIGSATVNISSSGNIL
                     LGASLSAIDVNGDGYSDLVVGGEGNGSNPGNVFIFHSTGPTGISATASVGNFASKITG
                     FANNSHLGSSVSTGDFNCDGFGDFIAGGQDYNFSGAQGHIIVGISNGSSGITFTTYSG
                     ANNNIASASSPSLFGISVGALDFNGDGCTDIFAGGHTTGASVQGALYYFQSAGTGFAS
                     INSASQANAVLTGINLNDKFGQSLAK"
     gene            complement(1215..1742)
                     /locus_tag="PK683_09605"
     CDS             complement(1215..1742)
                     /locus_tag="PK683_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08746.1"
                     /translation="MMPDTKISLQEFKELVQPEFYREQKGELKAAEISEAPIWEKMQT
                     QLFRVEDRLIQFYAVHNRKITLLGGEFSCSLRTAVCDLDNDGKYECIYTYEYGSGVSV
                     GVLGIYSPTEKMEIGKSCLIPPGNSMANFVFEKKSDRRVDVYVDAMNEKIMQGTLILK
                     SSDGRKILEIGQDTL"
BASE COUNT          587 a          436 c          514 g          643 t
ORIGIN      
        1 ggtgattgga ggaaaggtat atataggtac tgagggaaga gtatggattt ttcattctca
       61 aggaacgact gggattgcaa gcaatactat tggttctgca acggtgaata tatcgtcatc
      121 cggaaatatt ttactaggcg catctttatc cgcaattgat gtgaacggag acggttactc
      181 tgatttagtg gtcggtggtg aaggcaatgg ttcgaatcca ggcaatgtct ttatttttca
      241 ttcaacaggt ccgaccggaa tttcggctac tgcatccgtt ggaaattttg ccagcaaaat
      301 aaccggtttt gcaaataaca gccatttggg cagttctgtt tctacaggtg attttaactg
      361 tgacggattc ggagatttta ttgccggcgg ccaagattat aatttttccg gtgcccaagg
      421 tcatatcatc gtcggaattt ccaacggcag ttcagggatc acttttacaa cctattccgg
      481 cgcaaataat aatatcgcat cagccagcag tccctcttta ttcgggattt cagtcggagc
      541 cttggacttc aatggagacg gctgcactga catcttcgca ggcggtcata caacaggagc
      601 ctcagttcag ggcgcattgt actatttcca gtctgcgggc actggatttg catcaatcaa
      661 cagtgcaagc caggccaatg ctgttttaac tggaataaat ctgaatgata aatttggaca
      721 gtccttagca aagtagttca tcttttgcct gcggcgattt gaggactcca gctttgaaaa
      781 ctgcagagtc ccacgcgtta tcagcaaggt tatggagcgg acgaatcccc agcagtacaa
      841 acggagaaaa accacagaga aagtggagtt cccacaggcc ggagaatgga gattggcgtc
      901 ttagcggcga attcccgttg gatgatgttc gtattgcaaa caggagtcgg aaacccgcca
      961 gagggcagga atttgcgttt ttagaggggt tggggggagt tcctacaaac tgagggagga
     1021 tgggaggaaa gactttcatt tcccgcaata tcaataagat taatcgaaat tcgaggacta
     1081 ttgaatattg cacgttatgt atgagcataa atctcctgat ttttccccgt ttggaaacat
     1141 cagtctctgc tgagtgattt gcataatcct caatattcta aaaggaatac aatgttatat
     1201 agaatatagt ataattacaa agtatcctgt ccgatctcca gtatcttccg tccatcggaa
     1261 cttttcagta ttagagtccc ctgcattatt ttttcattca ttgcatccac atacacatcg
     1321 acccggcggt ctgatttttt ttcgaacaca aaatttgcca ttgagtttcc cggagggatg
     1381 aggcaggact tgccgatttc cattttttct gtcggggagt agattcctaa gacgccgacc
     1441 gaaacgcctg aaccgtattc gtaagtgtat atacattcat atttaccgtc attgtccaga
     1501 tcgcagactg cggtcctcag gctgcagctg aattctcctc cgagcagtgt gatttttctg
     1561 ttatggactg cataaaactg aatcagtctg tcctcaactc tgaacagctg tgtctgcatt
     1621 ttctcccaga taggggcctc tgaaatttcc gctgctttca gttcaccttt ctgttctctg
     1681 taaaattcag gctgtacgag ttctttaaat tcctgaaggc tgatttttgt gtccggcatc
     1741 attgtctcct gaattaggaa tgattctgag gtcagtgctt ttttcatctc ttttttggga
     1801 tttgaaggtg agctttgttt tagggagagt tggaacacgc ttataaatga gacataatct
     1861 gcagatgaac ctgtggaaaa aaaggagttc ccacatttgg aactgacggg ttgcggaaat
     1921 tcgtcggcga gataagagac ataatcgagc agatcaattg acccaagtgt tggtggcata
     1981 atttagaata atgagacata attcgccagg aagagactat atagagaatg gaattcccac
     2041 atcctagtaa tgagacataa ttcggtagat tcagtgactc aagtatcggt gtcctaattt
     2101 gtattaatga gacataactt ataagtatga gtatctctta aaaaatggag ttcccatatt
     2161 tggaactggc gggtcggcgg
//
LOCUS       DAOQGJ010000566         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33545_length_2179_cov_2.444444, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000566 DAOQGJ010000000
VERSION     DAOQGJ010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33545_length_2179_cov_2.444444"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1676)
                     /locus_tag="PK683_09610"
     CDS             complement(147..1676)
                     /locus_tag="PK683_09610"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH08747.1"
                     /translation="MYIAFKCNFFSGNRPPPPVKTVSEERKPETSAVPSETKEPAAAS
                     QNPSVPYSPRKYELKKKGLKIKDLESNGGKRYAVIIGINKYEDIQITSLEKARNDAKV
                     IADTLKKDGEFEEVFLMTDDIDKSDSERRYPTKINIEEKIESVLRNAQPEDLIVFFFS
                     GHGISDADNNSYLIAADTISSNELHSSGKEKRYATAVNVSEQITKFKAKGIKKLLLMI
                     DACREKMNTTKSVSREELLKEYTDSEILATFYATGSGYYSYEDDESEYGVFSRYVVQG
                     MEGKADSNADGVVTFTELKAYVEKGVSKWSLRKNKQQKPFTKLDNEMNGDLAITFSGK
                     NSESRINVIGVRDEKRKNFYKSAVLPGWGQFARGDKIRGVLYGTAFFAGLGALASSYS
                     QFNHSRENYISKRNQDLLFSTNQVAYGQFGLLSYMNTQHAKSEIQNHANTGNLIQFGI
                     LAFYAWNLFDAYFLSKPKDPDAASTLNSFNPFGFQLYSRQTILPTGIDRQMEMKYAFE
                     F"
BASE COUNT          574 a          459 c          441 g          705 t
ORIGIN      
        1 ttgcttcgca agctgagtgt gatcctcccg gagggccaaa gggacttcgt cccttaggaa
       61 tccctgtctt tgcagggagt tctaatcctc gaggttttca tagcaacaag ccagcccatt
      121 cccaattccc ttttggccat tccctattaa aactcaaatg catatttcat ctccatctgt
      181 ctgtcaattc cggttggaag aatggtctgt cggctgtaaa gctgaaatcc gaatgggttg
      241 aaactgttca gagtggatgc agcatctggg tctttcggtt tggaaaggaa atatgcatca
      301 aaaagattcc aggcgtagaa ggccaatatc ccaaactgta tcagatttcc tgtatttgca
      361 tgattttgta tttcagattt ggcgtgctga gtgttcatat agctgagaag tccgaactga
      421 ccataagcga cctgatttgt actgaaaagc agatcctggt ttctttttga gatgtaattt
      481 tccctggagt gattgaactg gctgtaagag gaggcaagtg cccccagacc ggcaaaaaag
      541 gcagttccgt atagaactcc gcgaattttg tctcctctgg caaactgtcc ccatccgggg
      601 agaactgctg atttataaaa attcttacgt ttttcatctc tcactcctat gacattgatt
      661 ctgctttcgg aattttttcc ggagaaggtg attgcgagat ctccattcat ttcattgtcc
      721 agtttggtga atggtttctg ctgtttgttt tttcgaagtg accatttgct tacacctttt
      781 tccacgtatg ctttcaattc cgtgaatgta actacaccgt ctgcattgga atcggcttta
      841 ccttccattc cctgcacgac gtagcgggag aatacgccgt attctgattc atcatcctca
      901 tagctgtaat atccggaacc agttgcatag aatgttgcca gaatttcaga atctgtatac
      961 tctttcaaaa gctcttcccg gctgacactt ttggttgtgt tcattttttc acgacaggca
     1021 tcaatcatta aaaggagctt tttaattcct tttgctttga attttgtaat ctgctcggat
     1081 acatttactg cggtggcata tcttttttct tttcctgagg aatgaagttc atttgaagag
     1141 attgtgtcgg ctgcaatcag ataggagtta ttgtctgcgt cagaaattcc atgaccggaa
     1201 aaaaagaata caatcaaatc ttcaggctgt gcattgcgaa gaacagattc aattttctct
     1261 tcaatattga tttttgtcgg atatcttcgc tcagaatccg atttgtcaat atcatccgtc
     1321 attaaaaaaa cttcttcaaa ttctccgtct tttttcagag tatctgcaat taccttcgca
     1381 tcatttcttg ctttctccaa agatgtgatc tgtatgtcct catatttatt gattccaatg
     1441 ataacagcat atctttttcc tccattgctt tcgaggtctt tgattttgag accctttttc
     1501 ttcagttcat attttctagg ggagtaaggc actgagggat tttgcgaagc cgccgctggc
     1561 tcctttgttt cagagggaac tgctgatgtt tccggttttc tctcttcaga aactgttttt
     1621 acgggggggg gcggacgatt tcctgagaaa aaattacatt tgaatgcaat atacatataa
     1681 tatatccaat aattcgaata cagaaatttg gtcgcgggtt gctattaact gaaatctctg
     1741 aggaatgaaa tccctctgaa attcctgtcc tatacagttt cgtctcgcga agcgagccgt
     1801 aaacctgtac agacattttc actgcacaca gcggaaggct tcttcgaaaa tgctcccgca
     1861 gtcccctgaa ccctgaactc caggccctga tcttctcaag gcgcataatt gcagtcatcc
     1921 catttggata catcaaaatt acattcctga ctttgcacat agagagatcg cagataattc
     1981 agtacagaaa tactatctgt tgaagtacta aaattgcagg aagagagcag gcaaagaaac
     2041 aaatagacac ttatcttcat agtttctttg tacagcttat tataaatttg tcaatgattt
     2101 tgcagaccag aagctgaaaa agataaggat tggggcggtt atgagacaaa aactcattga
     2161 acgaaatatg ttgggcaga
//
LOCUS       DAOQGJ010000567         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33560_length_2178_cov_3.634008, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000567 DAOQGJ010000000
VERSION     DAOQGJ010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33560_length_2178_cov_3.634008"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..>2178
                     /locus_tag="PK683_09615"
     CDS             169..>2178
                     /locus_tag="PK683_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08748.1"
                     /translation="MHNWKISKKLYSLTGLFFIILLFSSIFSIFSAGKIMDRLETILA
                     DRLIPIYQLSQINTLTEDSFQNILSSMLHDPRLKENERHMRDHGIRQHIDKIEKNMQN
                     SDKIWKEYMSTFLTEKEKEYAKEYSSDRDELINKSFLKALMFMREGRFEELNLFIADT
                     LLPAYRKSKEHFDRLADLQIEVGNQEGRDAHSIYRTVIAGLIIVLIAGAAVLGVTALR
                     IIFSIVTPLQSLSDVTQKISSGSLSTSLEEYAAKNEIGILYNSFGKMLNAVKTLIQEM
                     NRMSSEHEKGEIDAKIDEEKFPGEFRSMAKGINDMVLAHITMNRKAVNIFDQFGKGNF
                     DAVLEKLPGKMVFVNEAVEQVRANLKSIIADTDLLVQAALEGKISTRADEKKQNGDFR
                     KILAGINRTLESITAPINETSEVLLQMANGDLRVKVKGQYKGDYLNIADALNKTIVSF
                     NSVLFQIQNSSAQVLQSSKQLADASQALASDSGEQASNLEEITATVMQIESQAKENSH
                     RAEEASLKSKEAKESAETGNSEMQKMLRAMDEINTASASIARIIKEIDAIAFQTNILA
                     LNAAVEAARAGEYGRGFNVVAEEVRNLASRSARAAQETAALIEETQKKISGGAKIAEE
                     TAGSLVKIVSGVSQVSKLVEDIAAASKEQSEGINQLNTGLNQINQV"
BASE COUNT          721 a          416 c          506 g          535 t
ORIGIN      
        1 tatgcagaat atattcagcg ttctcaacag tctggaaaag gacagtccct tatgataatt
       61 atcaactgct ggataaagaa taaaattatt gtataattgt tgaccttatg ctacacaaga
      121 cctcagagga ttcataaaaa aaaatatcct cgtttcggag actagactat gcacaattgg
      181 aagatttcta aaaagctgta ttcccttacg ggactttttt tcatcattct tctcttttcc
      241 agcatcttca gtattttcag tgcgggaaaa ataatggaca ggcttgagac aattctcgca
      301 gacagactga ttccaatata ccagctttct cagataaaca ctctgaccga agacagtttt
      361 cagaacatcc tttcttccat gcttcatgat ccaagactga aggaaaatga aagacatatg
      421 agagatcacg ggatcagaca gcatattgat aaaatagaaa aaaatatgca gaattccgat
      481 aaaatctgga aagagtatat gtctactttc ctgactgaaa aggaaaaaga atatgcaaag
      541 gagtattctt ctgacaggga tgagcttatc aataaatcct ttctgaaagc actgatgttc
      601 atgagggaag gacgttttga agagctgaat ctttttatcg cagacacact tctgcctgca
      661 tacaggaaat caaaagagca ttttgacagg ctggcagacc ttcagattga agtggggaat
      721 caggagggca gagatgcaca ttccatttac aggactgtca ttgcagggct gattattgta
      781 ctcattgccg gagctgcagt tctgggcgtg acagctctca gaatcatatt ttccattgtg
      841 actcctcttc agtcactgtc ggatgtgact cagaaaatat cttccggcag tctcagtacc
      901 tctcttgaag aatatgcagc aaagaatgaa attgggatac tttataattc atttggaaaa
      961 atgctgaatg cagtcaagac tctgattcag gaaatgaaca ggatgtcctc tgagcatgaa
     1021 aagggagaga tagacgcaaa gatagatgaa gaaaaatttc ctggtgagtt cagatctatg
     1081 gccaagggga tcaatgatat ggttcttgct catattacaa tgaacagaaa ggcagtcaat
     1141 atctttgatc agtttggaaa gggaaatttt gatgcagtgc tcgagaaact tcccggcaag
     1201 atggtttttg taaatgaagc tgtcgagcag gtgagagcaa acctgaaatc aatcattgca
     1261 gatactgatc ttcttgtgca ggctgccctg gaaggtaaaa tttcgacaag ggcggatgaa
     1321 aaaaaacaga atggggactt caggaaaatt ttagccggaa tcaacagaac actggaatcc
     1381 attactgccc caatcaatga aacatctgag gttcttcttc agatggcgaa cggagatctc
     1441 agagtcaagg tgaagggaca gtacaaaggc gattatctga atattgcaga tgcgctgaac
     1501 aagacaattg tatcgttcaa cagtgtcctt ttccagattc agaattcctc tgctcaggta
     1561 cttcagagtt ccaagcagct tgctgatgca agtcaggctc ttgcaagcga ttcaggagag
     1621 caggcaagca accttgagga aataacggcc actgtaatgc agattgaaag tcaggccaag
     1681 gagaactctc acagggcaga agaagcctct cttaaatcaa aagaggcaaa ggaatctgcc
     1741 gagacaggaa attctgaaat gcagaaaatg cttcgtgcaa tggatgagat aaacactgca
     1801 tcggcaagca ttgcaagaat cataaaggaa atagacgcca ttgcctttca gacgaatata
     1861 cttgcattga atgccgcagt ggaagctgcc cgggctggag aatacggcag agggttcaat
     1921 gtagtagctg aagaggtaag aaaccttgcc tccagaagcg caagggcggc acaggaaaca
     1981 gccgcgctca tagaagagac ccagaaaaaa atttccggag gagcgaagat tgccgaagag
     2041 acagcaggtt cactcgtaaa gattgtttca ggagtcagtc aggtttccaa gcttgtggaa
     2101 gacattgcag cagcttcaaa ggagcagtct gaaggaatca atcagctcaa cacaggactc
     2161 aatcagataa atcaggtg
//
LOCUS       DAOQGJ010000568         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33565_length_2178_cov_3.211022, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000568 DAOQGJ010000000
VERSION     DAOQGJ010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33565_length_2178_cov_3.211022"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1272
                     /locus_tag="PK683_09620"
     CDS             133..1272
                     /locus_tag="PK683_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNH08749.1"
                     /translation="MTGLSENIAIVSFGISVFLSLALFHRKEQQIPSRILGFILTAYS
                     IAFLCRYLVLTESFLCPYLPLFFLPFIFLFGPAVYFYFRASLFGRSVEYSEIRKLLIL
                     PLLAEMIFAGLLFYFPEFRDVKAVVNQKGAVEIFSISFICTGIVHCSYFLRKAHKETE
                     RYRSEFEENYSSDELSSLFWLKIFMVFNVGMIIMYLLLAGLLHLGLSRIPVSPLEGVL
                     NMLLIYLILYYMIFNPKVFAIIEKEEPEAVPAPKTEKKYSKQAVSEEDRKNYISRIRI
                     FMEKEKPYLDDEISLSDLSESIGIPKHHFSMTVNSELNMNFFQFISFYRVKEAERLLT
                     NPEYSEYTILRIALDSGFQSKSAFNKAFKQFTGLTPAEYRQQKQD"
     gene            1364..>2178
                     /locus_tag="PK683_09625"
     CDS             1364..>2178
                     /locus_tag="PK683_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08750.1"
                     /translation="MRLITAAVFFSIIHCTNIGDVSSDKKQILNLSFLRTGTIVISGK
                     AVKGTLSSASVRVHPLGNDGKCAKTIVLSEGRTDQSGNYELKYFQSGRPVCVIVSPDT
                     NGSSKMYDEKSGKQISWTDSENSLVTVMNEPTAFANGKKIKNRLNISPISSLSLRRIE
                     ELVKSNTDPALLGSVTKRANKEIAFRLGLKGSFSGKELKNNDSAAKSATDRDIEDIND
                     LNIDIEDSSDPAALRMRLLLAGFSQLAYENRKDKTKETTDLDISNVIKAFTEDA"
BASE COUNT          660 a          455 c          452 g          611 t
ORIGIN      
        1 tttcatttta cagactctgc cgcagaatat ttctgcggga ttcttttgtt gcagtttata
       61 atggagacag gttatgatct ttccaaattc caaggtccaa tgccccggca gtgaaagaat
      121 acaggaattt caatgacagg gctttctgaa aatattgcaa tcgtcagttt cggtatatcc
      181 gtttttcttt cgcttgcact gtttcaccga aaagaacagc agattccgtc cagaatttta
      241 ggattcatcc tgactgccta ttcgattgca tttctatgca gataccttgt tctgacagag
      301 tctttcctgt gtccgtacct ccctcttttt tttctgccgt tcatcttcct cttcggaccg
      361 gcagtctatt tctatttccg tgcaagcctt ttcgggagat cggttgaata ttctgaaatc
      421 cgaaaacttc tgattttacc tcttctggca gaaatgattt ttgcaggact gcttttttat
      481 tttccagaat ttcgggatgt aaaggctgta gtaaaccaga aaggagcagt ggaaattttt
      541 tccatcagct ttatctgcac tggaatcgtt cactgctcct atttcctgag aaaggcacat
      601 aaagaaacag agagatacag atctgagttc gaagaaaact actcaagcga tgagctctcc
      661 agcctgttct ggctcaagat ttttatggtc tttaatgtcg gaatgattat tatgtatctc
      721 cttctggcag gacttctgca tctgggactg agcagaattc ctgtgtctcc tctggaagga
      781 gtgctcaata tgcttctgat ttatctgatt ctttactata tgatttttaa tccgaaagta
      841 tttgcaatca tagaaaaaga agagccggaa gcagtccctg caccaaaaac agaaaaaaaa
      901 tattccaagc aggcagtatc cgaggaagac agaaaaaatt atatcagcag aatccggatt
      961 tttatggaaa aggaaaaacc ttatctggat gatgagattt ctctttctga tctttcagaa
     1021 tcaattggaa ttccgaaaca ccatttttca atgacagtga attcagaact gaatatgaat
     1081 tttttccagt tcatcagttt ctacagagta aaggaagcag aaagactgct gacaaatccg
     1141 gaatattcag agtatacaat tctcagaatt gcactcgatt ccggatttca gtccaagtcc
     1201 gctttcaata aggcattcaa acagttcaca ggcctcactc cggcagaata caggcagcag
     1261 aagcaggact gacttttttt tagaaaaaac agtccttcct tgcaagcagg gacgatgccg
     1321 acatcgaaac ctgttatcct gaatctgttc acaaggggga gtaatgagac tgatcacagc
     1381 tgcagttttc ttttcaatta ttcattgcac aaatattggg gatgtttctt cggacaagaa
     1441 gcagattctg aacctttctt ttttaaggac aggaaccata gtcatttcag ggaaggcagt
     1501 caagggaaca ctgagttctg cttccgtcag ggtgcatcct ctgggaaacg acgggaagtg
     1561 tgcaaaaaca attgtactct cagagggtag gacagaccag agcggaaact atgaactgaa
     1621 atacttccaa tctgggaggc cggtctgcgt aatcgtatcc cctgatacaa acggttcctc
     1681 aaaaatgtac gatgaaaaat ccgggaaaca gatctcatgg actgattctg aaaattccct
     1741 tgtgactgtg atgaatgaac cgaccgcatt tgcaaacgga aagaagataa aaaacagact
     1801 gaatatctct cctatctcaa gcctcagtct gagaagaata gaagaactcg taaagtccaa
     1861 tactgaccct gctctgcttg gatctgtaac caaaagagcc aacaaggaaa ttgcattccg
     1921 gctgggactg aaaggttcat tctcagggaa agaattaaaa aataatgatt ctgcagccaa
     1981 atcagcgacg gacagggaca ttgaagacat caacgatctg aacatagata ttgaagacag
     2041 ctctgatcct gcagccttga gaatgagact tcttcttgca ggcttttcgc agctcgctta
     2101 cgaaaacagg aaagacaaaa caaaagaaac gactgatctg gacatcagca atgtcatcaa
     2161 ggcatttacc gaggacgc
//
LOCUS       DAOQGJ010000569         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33590_length_2177_cov_3.102733, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000569 DAOQGJ010000000
VERSION     DAOQGJ010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33590_length_2177_cov_3.102733"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK683_09630"
     CDS             <1..188
                     /locus_tag="PK683_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNH08751.1"
                     /translation="IYQIAVSEGARVKAGDTLVIIEAMKMETSIQAPEDGVIAEILVQ
                     KGDVVQTGSLIATIKKV"
     gene            190..1509
                     /locus_tag="PK683_09635"
     CDS             190..1509
                     /locus_tag="PK683_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNH08752.1"
                     /translation="MKKTFGFFLLLIVLVILSAIGLSAEDALPIAKNSRLEKLPSLGD
                     TLSTFWTSTGIYGFLFPQNGNWVQGWGRLLMINVGILLLYLAIKKGFEPLLLLPIGSG
                     AVLSNIPLAGISEAAVNPGEAAGILYYIYKMGIESGVFPLLIFMGVGAMTDFGPMIAN
                     PKTALLGGAAQLGIFVTLIGALLLSKIVPGIDFDIYDAASIGIIGGADGPTAIYLASR
                     LSPDLLGSIAVAAYSYMALVPLIQPPIMRALTTEAERKIQMKQMRYVGKTEKIVFPLI
                     VLGLCILLLPSASPLIGFLMLGNLMRESGVVERLSATAQNALINIVTIFLGLGVGSKL
                     SADKFLNVKTLGILVLGAIAFSIGTAGGVLMAKIMNLFSEDKINPLIGSAGVSAVPMA
                     ARVSHNEGLKADHNNYLLMHAMGPNVSGVIGSAVAAGVLLSLKAFSN"
BASE COUNT          628 a          432 c          463 g          654 t
ORIGIN      
        1 atatttatca gattgcagta tctgaaggag cacgagtaaa ggctggagat actcttgtca
       61 tcattgaagc tatgaaaatg gagacatcca ttcaggctcc tgaagacgga gttattgcag
      121 agattcttgt tcagaaaggt gatgtagttc agactggaag cctgatcgcc acaatcaaga
      181 aggtataaaa tgaagaagac attcggtttt tttcttttat tgatagtact ggtaattctt
      241 tcagcaattg gactttctgc agaagatgcc cttccgattg caaaaaactc ccgactggaa
      301 aagctgcctt cactgggaga tacactttct acattctgga cttccaccgg gatttatgga
      361 tttctatttc ctcaaaacgg aaactgggtc caaggatggg gcaggcttct gatgatcaat
      421 gtaggaattc tgcttcttta tcttgcaatt aaaaaaggat ttgagcctct tctgctgctc
      481 ccgataggaa gcggagcagt tctttcgaat attccactgg cgggcatcag tgaagccgct
      541 gtcaatccag gtgaggctgc aggaattcta tattatatat ataaaatggg aattgaatcg
      601 ggagtttttc cgctgctgat ctttatggga gtcggtgcaa tgactgattt cggtccaatg
      661 attgccaacc ctaaaaccgc acttcttggc ggtgcggcgc agcttggaat ttttgtgaca
      721 ctgatcggag cattactttt atctaaaatt gtccctggaa tagatttcga tatttatgat
      781 gcggcatcaa taggtattat cggcggagcc gacggaccga ctgctatcta tctggcctca
      841 aggctttcac ctgatttatt gggttcgatt gctgtcgcgg cctattccta tatggcactg
      901 gttccactga ttcagcctcc aattatgaga gcactgacaa cagaagcaga aagaaagatt
      961 cagatgaaac agatgcggta cgtcgggaaa acagagaaga tcgtttttcc attgatcgtt
     1021 ctgggactat gcattcttct tctgccctct gcgtccccac tgatcggatt tctgatgctt
     1081 ggcaatctga tgcgtgagtc cggagttgtg gaaaggcttt ctgcaacagc tcagaatgct
     1141 ctgatcaata ttgttactat ctttctcggt ctgggagtag gctctaagct ttctgcagat
     1201 aaattcctta atgtgaagac gctcggcatt cttgtacttg gagcaattgc tttttcgatc
     1261 ggtaccgcag gcggtgtact gatggccaag ataatgaatt tattttctga agacaagata
     1321 aatcctctaa taggatctgc cggggtttct gccgttccta tggcagccag agtttctcat
     1381 aatgaaggat tgaaggcaga ccacaacaat tatcttctga tgcatgcaat gggaccgaac
     1441 gtttccggcg tgataggatc tgccgttgca gcaggagtgc ttctgtctct gaaagctttc
     1501 agcaattaat atctgctgaa agcaaagaaa tctgaatact gccgatacac tgaatcaatt
     1561 atttaaaaag catttatatt ataagtatta caaaatataa atctttaaat ttatactcat
     1621 attcaaaagt ctttgcatat ttcaaactta ttgaggcagt aaaatcaagg attcctgata
     1681 caataagaga aattgctttc ggcgatccga atagctgacc ttaagcaaat acaatatgct
     1741 gtccagatgc agtaattttc aaaaaacatt agggaaatct gacagtttga cagactgcga
     1801 taaatcagta ataacagacg cttcgctgct tccatactca ttggcagccc actttctgac
     1861 ttggttaggg cgacggcagt tttcaaggct gcgaaccctc aagcttccga caggcattcc
     1921 tccaaaaacg tccctcctgc ctaaggtgag tgagcaaata attatgattt atataatgaa
     1981 ttttctactg aatatttttg aaattcctaa tgattcaagc ttgaagagat ttttaagcaa
     2041 agacttagac tgctgaactt ttcagaaaaa tcaaaaaaat gaatatttaa aaagttaaag
     2101 ttctcattga catgaaataa atgagaaata atctaaaccc actatgataa gactgcttac
     2161 ctctttcctt tttctct
//
LOCUS       DAOQGJ010000570         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33641_length_2176_cov_2.158416, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000570 DAOQGJ010000000
VERSION     DAOQGJ010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33641_length_2176_cov_2.158416"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..996
                     /locus_tag="PK683_09640"
     CDS             208..996
                     /locus_tag="PK683_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNH08753.1"
                     /translation="MKQLSVFFGLAFLISWIIWLPLWGVYFGMNLPVLPYHHGLGGLG
                     PIIASFLTTWIFPGKESVKSLAAKCIQITPLPYFTAALFSPFILVLGASVIGYYADGT
                     ELNLSGLLKSKEFPEFSLPLFFIYNLIFFGLGEEAGWRGFALPRFQEKMSPLRAGILL
                     TLFWALWHLPLFLYRPGYLSMGVAGAVGWVLSLLTGSILLTWFYNSSKGSILICAVFH
                     TAMDVVFTAEAAGKNIINYTGFLITVWGILTVFILLLENRNGVK"
     gene            998..>2176
                     /locus_tag="PK683_09645"
     CDS             998..>2176
                     /locus_tag="PK683_09645"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH08754.1"
                     /translation="MAFTVVDSILRDRAFRNEKKVAVARFALFSMAMMFDILAYLGFI
                     EYTAVRPSMTTLLMDVLVVVPSAVLLILLLRNVYHSLIKFFAITFDYSLIAMMLIFDP
                     TIPKGGQQIYWVILVASLFPFFMNLLRYSRTGTLYSSILTLALFTGVSFHYNQGEAEN
                     LVPMFFNLIMMLLIGYSITSSSRKMMEEANTKKMMERYLPPQLVSELYKKNASIEPGG
                     EMRKVTVLFSDIRSFTSISESMKPDEVVSFLNEYLSAMTDTVFHYGGTLDKFVGDAIM
                     TVFGAPVERGDDAVRAVQTAVSMIKALAEFNRLHNHLKAPLEIGIGIHTGEVIAGNIG
                     SEKRLDYTVIGDNVNLSSRIESLTKFYKCSILISQSTYDELKKEISEELKEAQADMEP
                     K"
BASE COUNT          570 a          450 c          471 g          685 t
ORIGIN      
        1 tataaatacg tgtattttaa ctatatttat gatatagaat attataatta gaatcattat
       61 attcctggac atcctgataa tatcctttgt ctttccggtt ttattccgga atgttccgga
      121 gcggactgtt ttgtgacctt ggacaaaaaa aacaagtgac agaataaaat tgcttttcta
      181 gaaagaatga gggagcactg agagaatatg aaacaattat ctgttttttt cggtctggca
      241 ttcttgattt cctggataat ctggcttcct ctgtggggag tctatttcgg tatgaatctt
      301 cccgtcctgc cctatcatca cgggctggga ggtcttggtc cgatcattgc atcttttctg
      361 acaacctgga tttttccggg aaaggaaagt gtaaaaagtc ttgctgcaaa atgcattcag
      421 atcactcctc tgccgtattt cactgcagct cttttcagtc cgttcattct tgttctgggg
      481 gcatcagtca tcggctatta tgcagacggt acagaattga atctgtccgg tttacttaag
      541 tcaaaggaat ttcctgaatt cagccttcct ctgtttttta tatacaatct tattttcttc
      601 ggacttggtg aagaagccgg atggcggggt tttgcacttc cccgatttca ggaaaagatg
      661 tcgcctctga gagcaggaat tttactgact ctgttctggg cactttggca tctccccctc
      721 ttcctttatc ggcctggtta tctttccatg ggagtcgcgg gtgcagtcgg ctgggtgctc
      781 agtctgctta caggaagcat tcttctgaca tggttttaca attcgtcaaa aggaagcata
      841 ctgatctgtg cagtatttca tactgcgatg gatgtcgttt tcactgcaga agctgcagga
      901 aaaaatatca ttaattatac tggattttta attacagtgt ggggaattct tacagtcttc
      961 atcctgctgc tggaaaatag aaacggggta aaataagatg gcattcactg tagtagacag
     1021 cattctgcgc gaccgcgcat tcaggaacga aaagaaagtt gcagtcgcgc gctttgctct
     1081 gttctccatg gcaatgatgt ttgacattct tgcctatctg gggttcatcg aatatactgc
     1141 agtcagacct tccatgacca ccttgctgat ggatgttctg gtcgttgttc cctctgcggt
     1201 tctgctaatt cttcttttgc gaaatgtata ccacagtctc atcaaatttt ttgcaatcac
     1261 attcgattat tctctgattg cgatgatgct gatctttgac cctactattc ccaaaggagg
     1321 gcagcagatc tactgggtta ttctggttgc atccctgttt ccatttttta tgaatctgct
     1381 ccgatattcc agaaccggca ctctgtactc ttccattctg actcttgcac tttttacagg
     1441 cgtatccttc cattataatc agggagaagc agaaaatctg gtcccgatgt ttttcaatct
     1501 gattatgatg ctcctgatag gatattccat cacatcctca agcagaaaga tgatggaaga
     1561 agcgaacaca aaaaagatga tggaacggta ccttcctccc cagcttgtaa gtgaactcta
     1621 taaaaaaaat gcaagtattg aacccggagg tgaaatgcgg aaagtgactg ttcttttttc
     1681 agacatcagg tcctttactt ccatttctga atccatgaag cctgatgaag tcgtcagttt
     1741 tctgaatgaa tatctttctg caatgactga tactgtattt cattatggag ggactttgga
     1801 taaatttgtc ggcgacgcaa ttatgacagt cttcggagca cctgtggaaa gaggcgacga
     1861 tgccgtccgt gcagtgcaga cagcagtatc catgatcaag gccctggcag aattcaatcg
     1921 gcttcacaat catctcaaag ctcctcttga aatagggatc gggattcata caggtgaagt
     1981 gattgcaggg aacatcggtt ctgaaaagag gctggactat actgtgatcg gggacaatgt
     2041 caatctttct tcaaggattg aaagcctgac taaattttat aagtgttcca tactgatttc
     2101 tcagtcgaca tacgatgaac ttaagaaaga aatttcagaa gaactcaaag aagctcaggc
     2161 agatatggaa cctaaa
//
LOCUS       DAOQGJ010000571         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33657_length_2175_cov_3.865566, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000571 DAOQGJ010000000
VERSION     DAOQGJ010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33657_length_2175_cov_3.865566"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2081)
                     /locus_tag="PK683_09650"
     CDS             complement(<1..2081)
                     /locus_tag="PK683_09650"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNH08755.1"
                     /translation="MTQVKVHFSHPMTAFGNPRTKTDQFRVSCTAKGKARWLDDRNYV
                     YEFEKPLEAGVECEFKLKDEVKTLSGAEITGRKIFSFNTGGPAVLDVRPYSGSVVDEN
                     QIFILRTDSDVDSATLSGNVYFMAEGNPDKIGAVFLKKEEAKEIIDSLFKGKADRKTF
                     FIKSKLRFPHGKQIKLVWGRSVKTKSGFANAQDQVQDFTVRENFKISLSCERENKNSA
                     CLPMLPMRLSFSSSVARELAEKVELKGSSGEVYPIEFDKYENGNLVNSVKFKPPFPEK
                     SKFTIHLPENFQDDSGRKPESGIFPMKTSTSDFPPLAKFPGRFGILELDADTSLPVTI
                     RNLEPGVKSKITGMGNGAAGSYFGKLTGSQAKISPESVLHWIKKVEKANREVSVFGDE
                     KTNVQSFALPKTEGDKAFEVLGIPLKSPGFYVVEIESTALAQSLLEKKKVMYVPTSVL
                     VTGMAVHFKTGRENSLVWVTSLKTGEPVNDAKITVYDCKGKNLFSGETDEKGLVFIKN
                     RLNDPKCSYETDYSYGFLVTAEKEKDFSFTHTSWEKGIENWRYKLSFNSDGNDVHAAH
                     TVMDRTLFRAGETLHMKHIFRRESMNGLEFPREKDLPESAEITHEGSGQKYTFKLKWN
                     ERGYSETVWKLPEEVKLGAYSVALKSGKDRFISTASFQVEEFRVPLMKGEILLPSENL
                     VRPSSLDVGLSA"
BASE COUNT          531 a          557 c          440 g          647 t
ORIGIN      
        1 gcagaaagac cgacgtccag gctggacgga cgcacaagat tctccgacgg cagcaggatt
       61 tctcccttca tcagaggcac tctgaattcc tccacctgaa atgatgcagt ggaaatgaac
      121 cggtccttac cgcttttcag agccactgag taggctccca gcttcacctc ttccgggagt
      181 ttccatacgg tctcggaata ccctctctca ttccatttca gcttgaacgt atatttctga
      241 ccgcttcctt catgagtgat ctccgcactc tcaggcagat ccttctcgcg gggaaattcc
      301 agtccgttca ttgattctct tctgaatata tgcttcatat gaagagtttc gcctgctctg
      361 aaaagagttc tgtccatgac agtatgagcg gcatgtacat cgtttccgtc tgaattgaag
      421 gaaagcttgt atctccagtt ttcaatccct ttttcccagc tggtatgagt gaatgagaaa
      481 tccttttcct tttcagcagt cacaagaaat ccgtaagaat agtctgtttc ataactgcac
      541 ttaggatcat tcagccggtt ttttatgaat acaagtccct tctcatccgt ttctcctgaa
      601 aaaagatttt ttcccttgca gtcatacacc gtgatttttg catcgttgac cggctctcct
      661 gtcttcagtg aagtgaccca gacaagggaa ttctctcttc ctgtcttgaa atgcacggcc
      721 atcccagtca caaggaccga cgtcggaaca tacatgactt tctttttttc gagaagcgac
      781 tgtgcaagcg cagtgctctc aatttccact acataaaatc ccgggctttt cagagggatt
      841 cccagaacct caaatgcctt gtcgccttcc gtcttaggaa gagcaaatga ctgcacattc
      901 gttttttcat caccgaatac ggaaacttcc ctgtttgcct tctccacttt tttgatccag
      961 tgcagaacgg attcaggact gatcttcgcc tgagatccag tcagttttcc aaagtaagat
     1021 cctgccgcac cgttccccat tcctgtaatt ttggacttca ctcccggttc gagattgcgg
     1081 attgtcacag gcagagatgt gtcggcatcg agttcgagaa ttccgaacct tcccggaaat
     1141 tttgcgagag gaggaaaatc ggaagtggat gtcttcattg gaaaaattcc ggactcaggt
     1201 tttcttcctg aatcatcctg aaaattttca ggaagatgaa tcgtaaattt tgacttctca
     1261 gggaaaggcg gtttaaactt cacgctgttg acaagatttc cattttcata cttgtcaaac
     1321 tcaatcggat acacttcacc tgaactgcct ttcagttcca ctttctccgc cagttctctt
     1381 gctactgaag aggaaaaact gagacgcatc ggaagcatcg gaagacaggc ggaattctta
     1441 ttctctctct cacagctgag gctgatcttg aaattttctc tgacagtgaa atcctgcacc
     1501 tgatcctgag catttgcaaa accggatttt gtctttaccg atctgcccca gacaagcttg
     1561 atctgctttc cgtgaggaaa cctgagcttc gacttgatga agaaagtttt cctgtccgct
     1621 tttcccttga acagcgaatc gatgatttcc tttgcttctt cttttttcag aaatactgcc
     1681 ccgatcttat ccggatttcc ttctgccata aaatatacgt ttccgctgag agtggcactg
     1741 tctacatccg agtcggttct gagaatgaat atctggtttt catccacgac actgccggaa
     1801 taaggtctta catccaggac tgcaggcccc cctgtattga aactgaaaat ctttctgcct
     1861 gtaatctccg ctcctgaaag agtcttcact tcatccttga gcttgaattc gcattccaca
     1921 cccgcttcga gaggtttttc gaattcatac acatagtttc tgtcatcaag ccatctggcc
     1981 tttccttttg cagtgcagct caccctgaac tgatctgtct ttgtcctggg gttcccgaag
     2041 gcagtcatcg gatgcgagaa atgcactttt acctgagtca cattctttac tgccccctga
     2101 ggcgaaaaac tgctgatatg gggtttatta tctgcgaaaa cagaaacgga aaaaatgagg
     2161 aatagcaaaa aacga
//
LOCUS       DAOQGJ010000572         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33676_length_2175_cov_2.291509, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000572 DAOQGJ010000000
VERSION     DAOQGJ010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33676_length_2175_cov_2.291509"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..717
                     /locus_tag="PK683_09655"
     CDS             100..717
                     /locus_tag="PK683_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08756.1"
                     /translation="MAQMIKMMLFFMIVFPIAGNCAQNVSSAEIRQTELGEDIELQLG
                     QSIHMKQEKIRIDSRINSEAELLFNYLDRCSSDCRLRIGIPLGKIPFETVVRYWRGCR
                     IYVQELTENVQGFRTVKFRVEKQENAKPIQENFINGKKVSEEECQSFLKTLKEIPNTW
                     FCAKTSRGGRTGFDGKDETGNIYEYRSVSENEKQINSITSKKNGK"
     gene            724..1488
                     /locus_tag="PK683_09660"
     CDS             724..1488
                     /locus_tag="PK683_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08757.1"
                     /translation="MHIIFIFILFTENFLYALPSAPEGLGVIGEKSVRFEGVFFPTAD
                     LELYKISGRGKMIRTGYQIGFAEEEQKDFAHIKKGELCIKNTSKGKRAVHFQDFAELE
                     YEGKYLIFWEFRNEYIKILMKTDSANIGNSPNHPEIGFYIKISDLQKAEFDAVPWIRV
                     LNQKDKFFNSAKEGLNLREEANVRSRRIIKITDEKYVIIPLKKTNGKWMEVRVQKRKR
                     KYCEDGYDIIEHEYRGWIKAMDDSGRPNVWFYPRGC"
     gene            1600..>2175
                     /locus_tag="PK683_09665"
     CDS             1600..>2175
                     /locus_tag="PK683_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08758.1"
                     /translation="MDSADFFEHFRISINNILLNRYIILFTVISVFQCDYAEETPVSD
                     DSCRKSFFEDFKYGQHYELFSVKGRTPKVYFHQNRCIDSSFSIKSRYQTKQYLLRGDT
                     VTVTQEKDGFLCSLYISSEGKSTAGWLEKSSLRAYAKSEKNTEPVRWTGEWKSVRSDK
                     ILRISLSRNAEALSFSGIDNSIQAVKETAAWH"
BASE COUNT          746 a          330 c          504 g          595 t
ORIGIN      
        1 attttggata tgtggaatcc ttgctggaga aagattcaag cagactgatt cagagcatat
       61 ttcatcggaa tagagtgagg aactgaatct gtagagagaa tggctcaaat gataaaaatg
      121 atgctttttt ttatgatcgt ttttcctatt gcaggaaatt gtgcgcagaa cgtatcttca
      181 gcggaaattc gtcagacgga actgggagaa gatattgaac ttcagctggg acagagcatt
      241 catatgaaac aggaaaaaat tcggattgat tccagaataa acagtgaagc ggaattgctc
      301 tttaattatt tggacagatg ttcctctgac tgcagactga gaatcggcat tcctttaggg
      361 aaaattccat tcgagacagt tgtccgttat tggagaggct gcagaatcta tgttcaggaa
      421 ttgacggaaa atgttcaggg gttcaggaca gtaaaattca gagtggaaaa acaggagaat
      481 gcaaaaccta ttcaggaaaa ctttattaat ggaaaaaaag tttccgagga agagtgtcag
      541 tcctttctga agacactgaa ggaaattccg aatacctggt tctgcgcaaa gacaagcaga
      601 ggaggcagga ccggttttga tggaaaggat gagacaggaa atatatatga atacagatca
      661 gtctcggaaa atgaaaaaca gatcaattcg atcacctcga aaaagaacgg aaagtaatat
      721 aatatgcata taatatttat attcattctg tttacagaaa atttcctgta tgcacttcct
      781 tcagctcctg aaggtcttgg agtcatcgga gaaaaatcag tcaggtttga aggtgtgttt
      841 tttccaacgg cggatttgga actgtataag atttcaggca gaggtaaaat gataaggaca
      901 gggtatcaga taggatttgc tgaagaagag cagaaggatt ttgcccatat caaaaaaggg
      961 gaactctgca taaaaaatac aagcaaagga aaaagagctg ttcattttca ggattttgcg
     1021 gaattggaat acgaaggaaa gtatctgatc ttctgggaat tcaggaatga atatataaaa
     1081 atactgatga aaacagattc cgcaaatatc gggaacagcc cgaatcatcc cgaaattgga
     1141 ttttatataa aaatttcaga tctgcagaag gccgaatttg atgctgttcc ttggatcaga
     1201 gtattgaatc agaaggacaa gtttttcaac agtgcaaagg agggattgaa tttaagagag
     1261 gaagcgaatg tcagaagcag gcggattatc aaaattacag atgaaaaata tgtgattatt
     1321 cctctcaaaa aaaccaatgg gaagtggatg gaagtaagag ttcagaaaag aaaaagaaaa
     1381 tactgtgagg atggttatga tataattgaa catgaataca gaggatggat aaaggcaatg
     1441 gatgattccg gaagacccaa tgtatggttt tatccgagag ggtgctgagt ttgtccgttt
     1501 tttacaatac agccggaaaa acaggtgtta ttctgtgcat atgttttctc tggaacagtt
     1561 ttatgtgaca gcattctgtt tttgaacggg atttgaaata tggattcagc tgattttttt
     1621 gaacatttca gaatctcaat aaataatata ttattgaaca gatatattat attatttaca
     1681 gtgatctcag tctttcagtg tgactatgcg gaagaaactc ctgtatcaga tgattcctgc
     1741 aggaagagtt ttttcgagga ttttaaatat ggtcagcatt atgaactttt ttcggtaaaa
     1801 ggcaggactc ctaaagttta ttttcatcag aacagatgca ttgacagttc attcagcata
     1861 aaatcccgtt atcagacaaa acagtacctg cttcgcgggg atactgtaac agttacacag
     1921 gaaaaagacg gattcttatg ttccctttat atttccagtg aaggaaaatc cacagctgga
     1981 tggctggaga aatcatctct ccgggcatat gcgaaatcgg aaaaaaacac agaaccggtc
     2041 agatggacag gagaatggaa atctgtcagg tcagacaaaa tactcaggat ttcgctttcg
     2101 cggaatgctg aagctctttc cttcagcgga atagataatt ccattcaggc tgtaaaagaa
     2161 acagcggcct ggcat
//
LOCUS       DAOQGJ010000573         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33692_length_2174_cov_2.820670, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000573 DAOQGJ010000000
VERSION     DAOQGJ010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33692_length_2174_cov_2.820670"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..533)
                     /locus_tag="PK683_09670"
     CDS             complement(249..533)
                     /locus_tag="PK683_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08759.1"
                     /translation="MKVKGKNKAVQILEILNGNSKRIIDLKLKTKALFEEGISLYFRR
                     DFESAKKKFESVLKEDPKDKAAEIYFGRCSYYSKNGIAPDWDGAEKLDFK"
     gene            1607..2173
                     /locus_tag="PK683_09675"
     CDS             1607..2173
                     /locus_tag="PK683_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH08760.1"
                     /translation="MDNPVLSTISAEEYFRIEETSEVKHEYFQGDMYAMAGAGSGHNL
                     IVSNLIRALGNQLRTSPCLVYPSDMRVQLNENSHYTYPDVTIVCDEPVFLNDRKNTLL
                     NPMMIFEVLSESTELYDRGKKFQAYRSISFLRNYILVSSESRQIEVFSRNADGLWLIL
                     DLKTEGKIQIPYLDCELAFDEVYEKLAL"
BASE COUNT          617 a          449 c          452 g          656 t
ORIGIN      
        1 acggtgagtt taggattcat tatttttctt tctcatttct gaaaaagtca gcttcacaac
       61 tccgatgact agaaaataaa ttcccagacc gaatacacat ccaaaaagaa gaattgttgc
      121 aaggctcatt tcaatctcca attgactcaa ttatactcat ttcccaaata agacaagcga
      181 aaaatggaat ccttttctga actaaggcaa tctcaccgca aagcggttcg cgggcagggt
      241 tgtctgaatc atttaaaatc cagcttctct gcaccgtccc agtcgggagc aattccgttc
      301 ttggaataat aggagcatct cccgaaataa atttcagcag ccttgtcttt aggatcttct
      361 ttaagaacag attcaaattt tttctttgca gactcgaagt ctcttctgaa atacagagaa
      421 attccttctt caaacaaggc ctttgtcttc agtttcagat caatgatcct ctttgagttt
      481 ccgttcagaa tttccagaat ctgcactgcc ttgttctttc ccttgacttt cacagtatcc
      541 accattctgt aatgatactg taactgctca gcctgacggc tttgcagtta ctcttaaccc
      601 acgtttcacg tgggatggta aagcctaccg gcggtttaaa gggactgcgt ccctttaaaa
      661 tccctgcttt ttatttatat tataatcata ctgagcagtt gtaagccagc agtgaagagg
      721 aataggattt agtcagaatc ttgagtctcg aagctgtgtt cactgcctgc acatgagatc
      781 aggatgggga ggaggggaag gatttggatt agacgatgtt tcattaaaat gaactccttg
      841 tattttacct cgcttcgctg ccccggctcg tatcccggcg agcatagcga ggcctgcggg
      901 ggttttgccc ttcgggggaa agggcggcca taacgatata tgatgctatg gagagaggaa
      961 gctttctgct gaagccacaa gtatcaactt tttaattaga tatttaggga tacactatat
     1021 ccttattttt ccttcacgca gatgaggtta ttcgatttgt cttttataaa aagcctgatg
     1081 ttatatgatt catctttgga cgcaggatgc atagcataaa ctttacatat ttcaatactc
     1141 tggaaatagg ttggctcagt tatcccttca ataattttcc aattcaatat tttttcaata
     1201 ctttgaggtt ttgtgaattc atatgcaaat cttggtgaat taccggtgtc tgtaatattc
     1261 caaaaagtct gccttgcgtc ggtcagaaga aacaattcag ggatgaaatt taatgttctt
     1321 gagcccttaa aaccgccggt aggctctctg ccaaaaccgc cccacctgcg taaggtgaga
     1381 tagtaatatt ttataggtat atttttgtat agtattttta agaagagatt taaacgaact
     1441 atacaaaccg ctcccttagg ttacggtcac tgaactgcac ctaagcagta ccggcggctt
     1501 cgagaacctc agccaccgtc gagagccgtc agagtggaca ccagctgatt ccgcccaagt
     1561 catacaatac ttgacagaat ataaatctgc ccgaactctg atgattatgg ataatcctgt
     1621 cctcagtaca atcagtgcag aggagtattt tcgtatagaa gaaacttcag aagtaaaaca
     1681 tgaatatttc cagggcgata tgtatgcaat ggcaggagca gggagcggtc ataatctgat
     1741 tgtgagcaat ctgatccgag ctttgggaaa tcagcttcgg accagtccct gtctggtgta
     1801 cccatctgat atgagagtgc agttgaatga aaacagtcat tatacttatc cggatgtaac
     1861 gatcgtatgt gatgaacctg tatttttgaa tgacaggaag aatactcttc tgaatcctat
     1921 gatgatattt gaagttctgt ctgaatctac agaactctat gaccgaggaa aaaaatttca
     1981 ggcgtaccgg tcgatttcct ttctccgaaa ttatattctg gtctcgtctg agtccagaca
     2041 aatcgaagtt ttcagcagga atgcagatgg actctggctg atcctcgatt tgaagacaga
     2101 aggaaaaata cagattccct atctggactg tgagcttgcg ttcgacgaag tctatgaaaa
     2161 attggctttg taaa
//
LOCUS       DAOQGJ010000574         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33740_length_2172_cov_4.070855, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000574 DAOQGJ010000000
VERSION     DAOQGJ010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33740_length_2172_cov_4.070855"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PK683_09680"
     CDS             <1..289
                     /locus_tag="PK683_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNH08761.1"
                     /translation="SEIREKEFARNETQSERKFCPESELVVKEESESLMTQLKKLPQN
                     YKIPIMLYYFENMSYQEISEKLNLKMNTLKSHILRGKELLKNLLQNEKKSI"
     gene            270..599
                     /locus_tag="PK683_09685"
     CDS             270..599
                     /locus_tag="PK683_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08762.1"
                     /translation="MKKNPFDHPDIFDVYKPAPSGFRHTVLLNIIPDIFLTFSFWTLI
                     FLLAAPFAIFFISPEISAEKDLVAYVLLTYSFFFLLYAVLAGYIISNFRNSRMQDIKD
                     RFGYHEK"
     gene            629..973
                     /locus_tag="PK683_09690"
     CDS             629..973
                     /locus_tag="PK683_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08763.1"
                     /translation="MSLYSEEKKVTASEIMQMQTDSAFEVIGRLNKTETAALITQIRA
                     EAKKEYKDIDKFYLLISHLEKIQSVDEEQKRLKDLNLVYLLALGLFSGFLLYVYFRQR
                     KMIQSLERHIQN"
     gene            975..1898
                     /locus_tag="PK683_09695"
     CDS             975..1898
                     /locus_tag="PK683_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004768580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08764.1"
                     /translation="MKLDEIQGQETALQYLRTYIQNPGKIPPLLIFHGPSGTGKWSGA
                     ERFSFTVLCEKGTGCGHCISCKSFYQHQHPDYIQFPAGERVPIGDEKNPEEFTIRWLL
                     SKRIAYQPHLSSYRIVLFPNAGMINNEAETALLKTLEEPPSHTRFIFITEKLEDLKQT
                     ILSRGVSIGFGYLSRNTVRQITDSLSIPYEEYFGGSVNPLNCPPEVLNLIRTKIEELS
                     GSGISLIELENWIRNYKSDHEEWEENFSYPEFLEAFTAVLIHHLSKSSHRNRDQILEK
                     IFLFKENIHKDIPGLDHFIISRLFFDLSVLV"
BASE COUNT          666 a          462 c          433 g          611 t
ORIGIN      
        1 gtcggaaatc cgcgaaaaag aattcgcaag aaatgaaaca cagtctgaac ggaaattctg
       61 tccggaatcg gaacttgtcg taaaggaaga aagtgaaagc ctgatgactc agctgaaaaa
      121 acttccgcag aactacaaga ttcctattat gctgtactat ttcgaaaata tgtcctatca
      181 ggaaatttcg gaaaagctga atctgaaaat gaacacactg aagagtcata tattgagggg
      241 caaggagctc ctgaagaatc tgctgcaaaa tgaaaaaaaa tccatttgat caccccgata
      301 ttttcgacgt ctacaaaccc gctccttccg gattcagaca tacagttctt ctgaatatta
      361 ttccggatat attccttacc ttttctttct ggacactgat atttcttctg gctgctccat
      421 tcgccatatt cttcatcagt cccgaaattt ccgcagagaa ggacctcgtt gcatatgttc
      481 ttctgacata cagtttcttc tttcttctgt atgctgtttt agcaggctat attatcagta
      541 atttcaggaa ttccagaatg caggacatca aagacagatt cggttaccac gaaaaatgag
      601 acttcttctt attttactga tgactggttt gtcattatat tccgaggaaa aaaaagtcac
      661 tgcatcggag attatgcaga tgcagaccga ttcagcattc gaagtcatcg gcagactgaa
      721 taagactgag acagctgctc tgattacgca gatccgtgca gaagccaaaa aagaatacaa
      781 ggatatcgac aagttttatc tcctgatttc ccatctggaa aaaattcagt cagtggatga
      841 ggaacaaaaa agactcaagg atctgaatct tgtatatctt cttgcacttg gactgttttc
      901 cggtttcctt ctctatgtat atttcagaca gaggaaaatg attcagtccc tggaaagaca
      961 tattcagaac tgaaatgaaa ctcgatgaaa tacagggaca ggaaactgct cttcagtatt
     1021 taagaaccta tattcagaat ccgggaaaaa tcccgccgct tctgatcttt cacggtccct
     1081 caggtacagg caaatggtcg ggagcagaaa gattttcatt taccgttctg tgcgaaaagg
     1141 gaaccggatg cggccactgc atttcctgca agtcattcta tcagcaccag catcctgact
     1201 atattcagtt tcctgcagga gaaagggttc cgatcggtga tgaaaaaaat ccggaagaat
     1261 tcaccatcag atggcttctt tccaaacgaa ttgcatatca gcctcatctg agcagttaca
     1321 gaattgtact ttttcccaat gcaggaatga tcaataacga ggcagaaacc gcccttctga
     1381 aaactctgga agaacctccc tctcatacca gatttatatt tatcactgaa aaactggaag
     1441 atctgaaaca gacaatactg agccggggag tgagcatcgg gttcggctat ctttccagaa
     1501 atactgttag acagatcact gattcactgt ccatccctta tgaagagtac ttcggaggaa
     1561 gtgtaaaccc gctgaactgc ccccctgagg ttctgaattt aatcaggacc aaaattgaag
     1621 aactttccgg atcagggatt tctctgattg aactggaaaa ctggatcaga aattacaaat
     1681 cggatcatga ggaatgggaa gaaaattttt cgtacccgga attcctggaa gcattcactg
     1741 ctgtcctgat tcatcatctc tccaagtctt ctcacagaaa cagagatcag attctggaaa
     1801 aaatatttct gttcaaagaa aatattcaca aagatattcc cggtctggat catttcatta
     1861 tcagcaggct tttctttgat ctttcggtcc tggtataaaa ttcccttcac gggaagttca
     1921 gattaatctg aaatattttc aatgtctctc tttgaagaaa gtctaatcct gaatatttta
     1981 taattatata atgtgtaaac ttgactcaac ttatcccttc ggcttcagat ggggcgtttt
     2041 tagcagagag gctccggcgg tttcgcaaac tttgaaggtg ccgcaaactt tgaaggtgct
     2101 aagggctctg ccctcgctaa accttatgaa gttagctgcc aaaccttatg aagttagctg
     2161 ccagtctgaa ag
//
LOCUS       DAOQGJ010000575         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33800_length_2170_cov_4.669504, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000575 DAOQGJ010000000
VERSION     DAOQGJ010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33800_length_2170_cov_4.669504"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PK683_09700"
     CDS             complement(<1..653)
                     /locus_tag="PK683_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNH08765.1"
                     /translation="MNPVKYLSDKVGEHSKGYKMLIVRTYSVVLKNVLTDGFPYLMKG
                     LYYAARGKKNEQYRAFTHGSMTWGRTVIKNTKTTVYRSNEITIPETGHMIYLNHVNEL
                     DFPFDCCVVNKPFLANQTIKSTYLAYWWMKAMGSQVFDTSHQRTIAVSVRNLMAGLKD
                     YSYIVYPEGQNTYSEDIKPLKKGMVKLAFENKIPVAVVLKSGVRKLQEKQSGNVIGY"
     gene            complement(653..1681)
                     /locus_tag="PK683_09705"
     CDS             complement(653..1681)
                     /locus_tag="PK683_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNH08766.1"
                     /translation="MSHHGGNNTKPVIVAILANAVIAVSKAVGYFFSGSSALLSETIH
                     SIADVMNQALLLIGIKRGSLGENEMFHYGHSQERFFWNLVSAMGIFVLGCGVTVYHGV
                     HDLLHPTPFKQAGAEQYVIEIILLISLVMESYSFYVAVGEIRIQAQTRSKKFFAYLAE
                     STDPSISAIFWEDLAAISGILLALFGIVLTNLTGDKTFDSAASILIGLLMGWIAYHLA
                     IENKRFLTDRSIPDFEMRAIAKMLDDDKMIKSCSEIRTVVLGPDRLKFSAKVELDMKE
                     LLPAVIEAELTKHSKGKKTGVKPEELKTILSEYSVDLYEVQKKEIARVTKELESKVPN
                     LKHVELRI"
     gene            complement(1685..>2170)
                     /locus_tag="PK683_09710"
     CDS             complement(1685..>2170)
                     /locus_tag="PK683_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08767.1"
                     /translation="PDFSKLKKAQAKLGFFKSRFRISPFFEGKGLTYKQTDINFETDF
                     YNEFLIFPATNLTEVINKWIQDSELAEYTPFNLADEENTLLAGNINALYGDFRSEKSA
                     KAVLELEMYIQKSKSDKLAFRKIYRKEISLTKPTPENLVIGWNQALTEILNQFTNDIR
                     K"
BASE COUNT          535 a          461 c          458 g          716 t
ORIGIN      
        1 ctgtagccga tgacatttcc cgactgtttt tcctgaagtt ttctgacccc tgatttcagc
       61 acaactgcaa caggaatttt attttcaaat gcgagtttga ccattccttt tttcagaggt
      121 ttgatgtctt cgctgtatgt attctgtcct tcaggatata cgatatagct gtagtccttc
      181 agtccggcca tcaggtttct tacggaaact gcaattgttc tctgatggga tgtatcgaat
      241 acctgggaac ccatcgcctt catccaccag taggcaaggt atgtggactt gattgtctgg
      301 tttgcgagaa aaggtttgtt taccacacag cagtcaaacg ggaagtccag ttcattcaca
      361 tgattcaggt aaatcatatg tcctgtctct ggaattgtaa tttcatttga cctgtagact
      421 gtggtttttg tgttcttgat gactgttctt ccccaggtca ttgacccgtg agtgaaagcc
      481 ctgtactgtt cattcttttt tccccttgca gcataataga gtcctttcat caggtagggg
      541 aatccgtctg taaggacgtt tttcagaaca acagaatatg tcctgacgat gagcatttta
      601 tagcccttgg aatgttcgcc gactttatcg gaaagatatt ttactggatt catcagattc
      661 taagctccac gtgtttcaga ttcggtactt tggattcaag ttcttttgtt actctggcaa
      721 tttctttttt ctgcacttca tacaggtcga ctgaatactc ggagagtatc gtcttcagtt
      781 cctccggctt gactcctgtc ttttttcctt tggaatgttt tgtcagctct gcttcaatga
      841 ctgcaggaag aagttctttc atatccagtt ccacttttgc agagaatttc agtctgtccg
      901 gaccgagcac cactgttctg atttcagagc aggatttgat cattttatcg tcgtcgagca
      961 tctttgcaat tgctctcatt tcaaaatctg gaatggatct gtcagtgagg aatcttttgt
     1021 tttcgattgc cagatggtat gcaatccagc ccataagcag accgatcagt atacttgcgg
     1081 cagaatcaaa tgttttgtcg cctgtgagat tcgtaagcac aataccgaat aatgctaaaa
     1141 ggatacctga gattgcggcg agatcttccc agaaaatggc gctgatggaa gggtctgtgg
     1201 attcagccag gtaagcaaag aattttttgc ttcttgtctg tgcctgtatt ctgatttcac
     1261 ccactgccac atagaaggaa taactttcca taaccagaga aatcaaaagt atgatttcga
     1321 tgacgtactg ttccgcgcct gcctgtttaa acggtgtcgg atgaagcaga tcatgcactc
     1381 cgtgatagac tgtcactccg catcccagta caaaaatccc cattgcagat acaagattcc
     1441 agaagaaacg ttcctgagag tgtccgtaat gaaacatttc gttttcgccc aggcttcctc
     1501 ttttgattcc gatgagaaga agggcctggt tcataacgtc tgcgatcgag tgaatcgttt
     1561 cagaaagaag tgcagatgag ccgctgaaaa aataaccgac tgcttttgaa actgcaataa
     1621 ctgcgtttgc cagaattgca actatgaccg gttttgtatt gttaccgccg tgatgggaca
     1681 tgttttattt ccttatgtcg ttagtgaact gatttaagat ttctgtcaat gcctgattcc
     1741 agcctatgac cagattttcc ggagtcggct ttgtcaggct gatttccttc ctgtaaattt
     1801 tcctaaaggc caatttgtca gattttgatt tctggatgta catttccaat tccaatactg
     1861 cctttgcaga tttttcactt ctgaaatctc cgtacaaggc attgatgttt cctgctaaaa
     1921 gagtattttc ttcatcggcg agattaaatg gagtatattc tgcgagttcc gagtcctgga
     1981 tccatttgtt aatgacttca gtcagatttg tcgctggaaa gatcagaaac tcattgtaaa
     2041 agtctgtttc aaaattgatg tctgtctgct tataagtgag gcctttccct tcgaaaaatg
     2101 gagaaatccg gaatctgctt ttaaaaaagc ccagtttcgc ctgtgctttc ttgagtttcg
     2161 aaaaatcagg
//
LOCUS       DAOQGJ010000576         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33895_length_2167_cov_3.114110, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000576 DAOQGJ010000000
VERSION     DAOQGJ010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33895_length_2167_cov_3.114110"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..1248
                     /locus_tag="PK683_09715"
     CDS             187..1248
                     /locus_tag="PK683_09715"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNH08768.1"
                     /translation="MDYHRKIKVAVIDDSPLVRNIISDLLQSDKNIEVIATGKTGLDC
                     IDVVEKHHPDIVTLDIEMPVMDGLTALEEISKRKLNVGVIMLSVLTQHGADATFRALE
                     LGAIDFVPKPSSAVRMNLEEIGQLLKSKILGYFDYEDKKKAPRVKIKVGNFPEIKKTT
                     PVRAIAIGTSTGGPNALQAVFKMFPEDFYYPVFIVQHMPAGFTKAFAERLNEISKIRV
                     KEAENGEIVQAGVAYIAPGNYHMKIHKRENNISIELDQGVPVNGHRPSVDVTFDSLNQ
                     TYGAGALSVIMTGMGKDGAESIKRLKEIGAPTLAQDEKTSVIFGMNRQAIENGGVDHV
                     VPLQEIVPQMIRIIKERGM"
     gene            1252..1614
                     /locus_tag="PK683_09720"
     CDS             1252..1614
                     /locus_tag="PK683_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08769.1"
                     /translation="MARILVVDDAKFMRTLVKEALTAVGHTIVGEAENGNIAIEQYKL
                     LRPDLVTMDITMREKDGIAATSEIIKMDPKAKVIMVTALGQEDLLAKAIKMGVKDFVV
                     KPFPPERLQQAAAKALGL"
     gene            1623..>2167
                     /locus_tag="PK683_09725"
     CDS             1623..>2167
                     /locus_tag="PK683_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNH08770.1"
                     /translation="MLESAPDSEEHLFKVQWSSPEGGFSEGPLTLLWSLIESYKVDIF
                     EVSLSKITEDFIRFIQLAKNLPIELSSEYAKMAASLVYLKSKALLPNPGFEEAEDEPS
                     LPKELVEKLLEFKKYQIAGTRLGHMDSIQAAVFRRESSQILIDFPEDENWLDLDLIQL
                     ISAFNGLLSRQIGEEAKPSLLL"
BASE COUNT          659 a          398 c          524 g          586 t
ORIGIN      
        1 aactctttac cggcaatttt aaggatgtga tcagtgccgt taagcctggc gacactaaat
       61 ttttaagcat gaacactgaa ttcatttatg aaggggacaa tctgattgga aatctgatct
      121 tccttcctga ctacgaagaa ctggtcaaat taattgccaa gctgaattga ggactctgaa
      181 ttgctcttgg attaccacag aaaaataaaa gtcgcagtta ttgacgattc cccattggtc
      241 agaaatatta tttctgatct gcttcagtct gacaagaata ttgaagtgat tgcgactgga
      301 aaaacaggac tcgactgtat tgatgtggtg gaaaagcatc atcctgacat agtgactctc
      361 gacattgaaa tgcctgtaat ggacgggttg actgccttgg aagagatttc caaacgcaag
      421 ctgaatgtcg gagtcattat gctttctgtt ctgacccagc atggagcgga cgctactttt
      481 agagcactgg aacttggtgc aattgacttt gttcctaagc cttcaagtgc agttcggatg
      541 aatctcgaag agatcggtca gcttctcaag tcaaaaatat taggctactt tgattatgag
      601 gataagaaga aggcccctag agtaaaaatc aaagtaggaa attttcctga aataaaaaaa
      661 actacgccgg tgagggcgat tgcaattgga acttccaccg gaggtccgaa cgcgcttcag
      721 gctgtattca aaatgttccc ggaagatttt tattacccgg tatttattgt tcagcatatg
      781 cccgcgggat ttacgaaggc atttgctgaa agattgaacg aaatttccaa gatcagagtc
      841 aaagaggctg aaaatggaga gattgttcag gcaggggttg catacattgc acctggaaat
      901 tatcatatga aaattcataa gcgagaaaac aatatttcta ttgaattaga tcagggagtc
      961 cctgttaatg gccataggcc atcagtggac gtgacttttg atagtctgaa ccagacgtat
     1021 ggggccggtg ctctgtcagt gattatgaca ggcatgggaa aagatggtgc tgaatccatt
     1081 aaaagactga aggaaatcgg agctccgact ttggctcagg atgaaaaaac ctcagttata
     1141 tttggaatga atcgtcaggc tattgaaaat ggaggagtcg atcacgtcgt gccgttacag
     1201 gagattgtgc cacaaatgat acgtatcatc aaagaaagag ggatgtaaca aatggccagg
     1261 attttggttg ttgatgacgc gaagtttatg cgtacattag ttaaggaggc tttgactgca
     1321 gttgggcata caattgtagg agaagctgaa aatgggaaca ttgccataga gcagtacaag
     1381 ctgctcagac cggatcttgt cacgatggat ataaccatgc gtgaaaaaga tggtattgca
     1441 gctactagtg aaatcattaa gatggatcct aaagcaaaag tcattatggt gactgcactt
     1501 gggcaggagg atcttcttgc aaaggcgatc aagatgggtg taaaggattt tgtagtaaaa
     1561 ccttttcctc ctgaaaggct tcagcaggcg gctgccaaag cattgggatt gtaatttaga
     1621 gaatgttgga atctgctcca gattcagagg agcatctgtt taaagtccaa tggagcagtc
     1681 cagaaggagg tttttcagag ggtccgctca cattgctttg gagtctcatt gaaagttata
     1741 aagtagatat attcgaagtc tctctttcca agataacgga agattttatt cgatttattc
     1801 agctggccaa aaatcttcca atcgaactaa gttcagaata tgcaaaaatg gctgcaagcc
     1861 tggtatatct gaaatcaaaa gcacttctac ctaatccagg ctttgaagaa gccgaggacg
     1921 agccgtctct gcctaaggag ttagttgaaa aacttctgga atttaagaaa taccagattg
     1981 caggcaccag gcttggacat atggattcca ttcaggccgc cgtattcagg agggaaagtt
     2041 cacagattct gatcgacttt cctgaagatg aaaactggct ggatctggat cttattcagc
     2101 tcatttcagc attcaacggt cttctttcga ggcagattgg cgaagaagcc aaacccagcc
     2161 tgcttct
//
LOCUS       DAOQGJ010000577         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_33976_length_2164_cov_3.730204, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000577 DAOQGJ010000000
VERSION     DAOQGJ010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33976_length_2164_cov_3.730204"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..>2164)
                     /locus_tag="PK683_09730"
     CDS             complement(20..>2164)
                     /locus_tag="PK683_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH08771.1"
                     /translation="SEALQDVPGFDFQHTYGIYPQLVHQRGLVGENNRTLVYVDGIAD
                     NNINEFGAIAGTIQFPLQNVERIEIVSGPSSSLYGANAFNGVINIITKDGKTSPGNQA
                     TAMYGGWEKSGQANKGYSMTLTSRGSVATLGGDHIQYSASGYFYKTEGPNFGGIGQQD
                     KKGYNPNDVSYGLERKFCGGQCSPDSKSVGYYWSPTYNNSQVETYNATAKFSYKGFRF
                     ETINWQYLQGEGTFANGTQQIDTKQRGFETKNALEARNLARLYGVALGNASLKGFSGS
                     HWDFRNNTVNAGYNHKIKEGLTLDTEATIRNTQVLSSSGEEYPNKNGWDAFYRPGDTS
                     QGTNYSRPDTSYQSETKLFYEPTSKVSTILGASAIHYVAAKDYGSAERYTFNNFSGFA
                     QAQYRPIDKIAFTAGYRYDEFSTFGKASSPRISMIISPTKNFTIKLLAGTGFRAPTVK
                     EMFSETKQRKPNGSLRPELLKTYEVGLGYNFMGKLYTSLHGYYNKVTNLILEVTTLDS
                     GLRNGAAPSGGFWQQNQNLGEARIYGAEWDNAWTMTDKLKLNLSYNYVDGKYMKLPSS
                     LPTSPSTAGRIGDNWKDDFRTAVYKQFTGTAAIPEEGKIPNIAPHKAFVGFTWKIFKD
                     LSLFTAVNMIDIRRTKATNPEKTTPGYKMFKLNLHWDNAFTQGMFLNLQVNNLANEQF
                     FDPGIRAAAGTYYPTRHPLEHRNVWLTVGYNF"
BASE COUNT          503 a          502 c          467 g          692 t
ORIGIN      
        1 taaatgcctg atccggagct taaaaattat acccaactgt cagccagacg tttctgtgtt
       61 cgaggggatg tcttgtcgga taataagttc ctgctgcggc acggatacca ggatcgaaaa
      121 actgttcatt cgcaaggttg tttacctgaa gattcaggaa cattccctgt gtaaacgcgt
      181 tgtcccaatg gagattcagt ttgaacattt tatatcccgg agttgtcttt tcagggttcg
      241 ttgcttttgt tcttcttatg tcaatcatat tcactgcagt gaacaggctc agatccttga
      301 agattttcca ggtgaatccg acaaatgcct tatgaggggc tatgttagga atcttccctt
      361 cttcggggat tgctgcggtt ccggtgaact gtttgtaaac tgcagttctg aaatcatctt
      421 tccaattgtc accgattctt cctgcagtgg aaggagaggt cggcagagag gaagggagct
      481 tcatatactt gccatccaca taattatagc ttaaattcag tttcagcttg tccgtcattg
      541 tccatgcatt atcccactct gctccgtaaa ttcttgcctc acctagattc tgattctgct
      601 gccagaatcc tccgctggga gcagccccgt ttctcagacc gctgtcaagt gtagtgactt
      661 ccagaataag atttgtcacc ttgttataat acccatgcag agatgtgtac agttttccca
      721 tgaaattata tcccaagccg acttcatacg ttttcagaag ctcaggcctc agactgccat
      781 taggctttct ctgttttgtt tctgaaaaca tttccttgac tgtcggtgcc cggaatcctg
      841 taccagccag aagtttgatc gtgaaatttt tggtcggaga aataatcata ctgatcctgg
      901 gagaagaagc ctttccgaag gtggaaaact cgtcgtatct gtatccggct gtaaatgcga
      961 tcttatctat cggacggtac tgagcctgtg caaacccgga gaagttgttg aatgtgtatc
     1021 tttctgcgct tccataatcc ttggccgcaa cataatgtat tgcacttgct ccgagaatgg
     1081 tggaaacctt tgatgtgggt tcataaaaaa gcttagtttc cgactgataa ctggtatccg
     1141 gtctggaata atttgtacct tgagatgtat ctcccggtct gtaaaatgca tcccatccgt
     1201 ttttattagg atattcctct ccgcttgagc ttaagacctg ggtgtttctg attgtcgcct
     1261 ccgtatccaa ggtaagccct tcttttattt tatgattata tcctgcattg actgtattgt
     1321 ttctgaaatc ccagtgtgaa ccggaaaatc ctttcagtga tgcgttgcct agcgcaactc
     1381 cgtaaagtct ggcaagattt cgtgcttcca gtgcattttt ggtttcaaaa cctctttgtt
     1441 tcgtgtcaat ctgctgtgtt ccgttcgcaa atgtcccttc cccttgcaga tactgccagt
     1501 tgattgtttc aaaccggaat cccttatagg aaaatttagc ggtagcattg tatgtttcca
     1561 cctgagagtt gttgtaggta ggcgaccagt agtaaccgac tgattttgaa tctggactgc
     1621 actgtcctcc gcagaacttt ctttccagtc catatgaaac gtcatttgga ttgtatcctt
     1681 ttttatcctg ctgtcctatc cctccgaaat ttggtccttc cgtcttgtaa aaatacccgc
     1741 ttgcactgta ctgaatatga tcacctccga gtgtcgcaac agaacctcgg gaagtcagtg
     1801 tcatactgta tcccttattt gcctgtcctg atttttccca gcctccgtac atggcagttg
     1861 cctgatttcc gggactggtt ttcccgtctt tagtaatgat atttatgacc ccgttgaagg
     1921 catttgctcc gtacagagaa gaagacggac ctgagacaat ttctattctt tccacattct
     1981 ggagagggaa ctggattgtg cctgcaatag caccgaattc gttgatgtta ttgtctgcaa
     2041 tcccatccac atataccagc gtacggttgt tttctccgac aagccctctc tggtgaacaa
     2101 gctgaggata aattccataa gtgtgctgaa agtcaaatcc gggaacatcc tgcagggctt
     2161 cgga
//
LOCUS       DAOQGJ010000578         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34049_length_2161_cov_3.525641, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000578 DAOQGJ010000000
VERSION     DAOQGJ010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34049_length_2161_cov_3.525641"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PK683_09735"
     CDS             <1..408
                     /locus_tag="PK683_09735"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNH08772.1"
                     /translation="VLIHNGDVSLTDYAYLKINSAARVWKSRTESFEKNKNKYPVDKL
                     YIMDCDIDIIQNVPWSRNWIPVLEDGGGNCICIDMEPGQKGKMGQILFWERETGPEAS
                     RFGSFAEWLSAYRDDLYNGKYKVDRDGFLNRID"
     gene            629..1045
                     /locus_tag="PK683_09740"
     CDS             629..1045
                     /locus_tag="PK683_09740"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNH08773.1"
                     /translation="MDDLKTVFGNDVFSQRMMELHVLKTLEKNWEKLVGKTFLYNSWP
                     VDFNQGRLKIVIAHDSYRMELEFLKAIFFRNANSLFPEPVIEHWSFRLGNPMSGRKIP
                     EKKKPNFTGKDELLKIAENEKDDEFRKKLIELIGLF"
     gene            1057..>2161
                     /locus_tag="PK683_09745"
     CDS             1057..>2161
                     /locus_tag="PK683_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004756774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08774.1"
                     /translation="MSQETYGAEKIKILEGLEAVRKRPGMYIGTQDESGLHKMVYEVV
                     DNSVDEAMAGHCTHILVKILPGNIIEVSDNGRGIPVDIHPEKKVSTIEVVLTILHAGG
                     KFENDAYKVSGGLHGVGVSVVNALSEWLEVEVHQRGNKYYQKYFTGVPDKPVEVVGPA
                     SMSGTTVRFRPDSSIFTTTEFQYDLLSSRFREMAFLNKGLNLRIEDHRKEEVHSDEFC
                     YEGGIVSFVDLLNQNKHPIHDKVIHFERQKEGMVAEIALQYADTYSENIFCFTNAINN
                     SLGGTHLEGFRTALTRTLNDYLKKDVNLSKKQPLGLSGDDVKEGLTSVISIKIPQPQF
                     NSQTKEKLVNAEVKGQMQTMVSEGLVNYFEENPS"
BASE COUNT          671 a          430 c          529 g          531 t
ORIGIN      
        1 gttctgatcc acaacggaga cgtttctctg acggactatg cctatctgaa aataaattcg
       61 gcagcaagag tatggaagtc aagaactgag tcttttgaaa agaataaaaa taaatatcct
      121 gtagataaat tatatatcat ggactgtgat atcgacatta ttcagaatgt tccctggagc
      181 agaaactgga ttcctgtgct cgaagacgga ggaggaaact gcatctgcat agatatggaa
      241 ccggggcaaa aggggaagat gggtcagatt cttttctggg aaagagaaac aggaccagag
      301 gcgtcccgct tcggctcatt tgcagaatgg ctttctgctt accgggatga cttgtacaat
      361 ggaaaatata aagtggacag agacggattt ctgaacagaa ttgactgact gcagtctgcc
      421 ctttcggaat ttgaaatttt acggaacgcg gatgattaaa acagaaatga acggacatcg
      481 atggtttttc cgttcaacga actgcaggga agcaaatatt cagctgaccc ggtttggaat
      541 cgaatgactt tgagtttctg aatccggaca atgcagagga ggacaaatca ttatttttga
      601 aagaaagaaa aacttcaagc gtcctactat ggacgatctt aagaccgtat tcggaaatga
      661 tgttttttct cagaggatga tggagctgca tgtgctgaag actctggaaa aaaactggga
      721 gaaacttgtc ggaaagactt ttctttacaa ttcctggcct gtcgatttca accaaggacg
      781 tctgaaaatt gtcatcgccc acgattccta ccgaatggag ctggagttcc tgaaggcgat
      841 attttttcgg aatgcaaatt ccctttttcc ggaaccggtg attgaacact ggagttttcg
      901 tctgggaaat cctatgtccg gcagaaaaat tcctgaaaaa aagaagccga attttacagg
      961 aaaggacgag cttctgaaga ttgcggaaaa cgagaaagac gatgagtttc ggaaaaagtt
     1021 aattgagtta ataggcctgt tttaaagact catcctatga gtcaggaaac atacggtgca
     1081 gagaaaatta aaattctgga aggtcttgag gcggtcagaa aacgcccggg aatgtacatt
     1141 ggaacccagg atgagtccgg tctgcataag atggtctatg aagttgtgga caactcagtg
     1201 gatgaggcaa tggccggaca ttgtactcat attctcgtca aaatccttcc cggaaatatt
     1261 atagaggttt cagacaacgg ccgaggaatt ccagtcgata ttcatccgga gaaaaaagtc
     1321 tccacaatag aagtcgttct cacaattctc cacgcaggcg gtaaatttga aaacgatgcc
     1381 tataaggtgt ccggcggtct gcacggggtg ggtgtaagcg tggtcaacgc actttctgag
     1441 tggctggaag tggaagtcca ccagagagga aacaagtact accagaaata ctttacaggt
     1501 gtcccggata aaccggtcga agttgtaggg cctgcatcca tgtcaggcac cacagtccgt
     1561 ttccgtccgg attccagcat attcaccacg acagaatttc agtatgatct tctgtcttcc
     1621 cgattcagag aaatggcttt cctgaacaag ggtctgaact tacggataga ggaccataga
     1681 aaagaggaag tccattcaga cgaattctgc tacgagggag gaatcgtttc cttcgtggat
     1741 cttctgaatc agaacaaaca tcctatccat gacaaggtca ttcattttga aagacagaag
     1801 gaaggaatgg tcgcagaaat tgccctgcag tatgcagaca catattctga aaacatattc
     1861 tgttttacaa atgcgatcaa caacagtctg ggaggtacgc atcttgaggg tttccggaca
     1921 gcactgacca gaacactgaa cgactatctc aaaaaagacg tcaatctttc caagaagcag
     1981 ccgctcggac tttccggaga tgacgtcaag gaaggactga cgtcagtgat ttccatcaag
     2041 attcctcagc ctcagttcaa ttcgcagaca aaggaaaaac tggtcaatgc ggaagtcaag
     2101 ggacagatgc agacaatggt atccgaggga cttgtcaatt atttcgaaga aaatccttcc
     2161 g
//
LOCUS       DAOQGJ010000579         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34122_length_2159_cov_2.046103, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000579 DAOQGJ010000000
VERSION     DAOQGJ010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34122_length_2159_cov_2.046103"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PK683_09750"
     CDS             complement(<1..675)
                     /locus_tag="PK683_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNH08775.1"
                     /translation="MKVANRVSDLIGHTPLVRLGRMASASKADVLVKLESFNPLSCVK
                     CRIARAMIDDAERQGKIKPETVIVEPTSGNTGIGLAYVCAERGYKLILTMPETMSLER
                     RQLLKIFGAELILTPGPEGMKGAIKKAEELLVTIPNSFAPQQFNNPANPEIHRNTTAE
                     EIWADTEGKVDIFIGGVGTGGTITGVAEVIKQRKPSFQAIAVEPFDSPVLSEGRAGAH
                     KIQGIGP"
     gene            1225..1662
                     /locus_tag="PK683_09755"
     CDS             1225..1662
                     /locus_tag="PK683_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH08776.1"
                     /translation="MIKKTAVAIFLITLNSLSADAVADIEKMFSEHRPKDLPSLASNL
                     SAQGSIHHIKGDYEKAQTFFQKSLKIRKQIGLEMSAGYANILYLSSIADFRMGKSCEA
                     KTKVTKVIEIYRYLGDSAEANQIESEGLGSFKKACEATMVAGN"
     gene            complement(1663..>2159)
                     /locus_tag="PK683_09760"
     CDS             complement(1663..>2159)
                     /locus_tag="PK683_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HNH08777.1"
                     /translation="HSMGSHLHFILAKDGGKKIINPLPFLPVIPDTSAPKIGMMILTN
                     VDKFTYVNDGDNINVSRAFPVSIPILDSGIRPGQRRGIQRVKFLLNGKVLKDAGFNEI
                     SLRENTWVNETGQSFEELFFLGNYFVGNLSFVSGENVLEVRTSDFSGNEAAKVIGFNV
                     TRIK"
BASE COUNT          642 a          475 c          386 g          656 t
ORIGIN      
        1 tggtccaata ccctggatct tatgggcacc tgcccttcct tctgaaagta ccggagaatc
       61 aaacggttct acggcaattg cctgaaagga gggctttctc tgtttaatca cttcagcaac
      121 acctgtaatt gttccgcctg ttcctacccc tccgataaaa atatccactt ttccttctgt
      181 atctgcccag atttcttctg cagtcgtatt tctgtgaatt tccggattgg caggattgtt
      241 gaactgctga ggagcaaacg aatttggaat cgtcacaagc agttcttccg cttttttgat
      301 cgcacctttc attccctcag gtcctggagt caaaatcagt tccgctccaa aaattttcaa
      361 aagctgtctt ctttccagac tcatcgtttc cggcatagtc agaatcagtt tatatcctct
      421 ttccgcacat acataggcga gaccgattcc agtattcccg cttgtaggct ctacaattac
      481 agtctccggc ttgatctttc cctgtctttc ggcatcatca atcattgccc tggctatgcg
      541 gcatttcacg caggacagcg ggttgaagga ttccagtttt accaatacat ccgccttgct
      601 tgcggatgcc attcttccca gacggacaag gggagtgtgt ccaatcagat cactcacacg
      661 atttgcaact ttcataaaaa acctccatat gcgaaaatcc aatatataat aatttactcc
      721 aatatattag atatatttcc caaatattaa acagggattc ttttgtaaac tttttttatc
      781 tgttatagtg aaaaaatgtt taaaactgag agaatgaatc tcaacacgca ttactgattc
      841 ggcagtttcg actattgcct atgagaaatg aggtgatact gattaccaaa agtaattgag
      901 gtttttacgt tgagaaacct tgattttact cgcagaacag ggttgaaata tgtaaaacct
      961 tttgaatatg attacaaaca aaagcaatat gcttatctaa tgggcataat ttttaagtta
     1021 atcagtagac gattgattcc tttataagca ttcgtcagat tattttataa atttcttcag
     1081 tattcttcag taaaatccta aataaggccc atattactgc atattttcct agtttgtaca
     1141 tttttctccc agtcaaactg cactgtataa aaatggcaca ctatttgctt attaaacact
     1201 atgagagtct ggaggacaaa aaacatgatc aagaaaacag cagttgctat atttttaatc
     1261 actttgaatt ctttaagcgc agatgcagta gctgatatcg agaaaatgtt ttctgaacac
     1321 agaccgaaag atctcccaag tctggcatct aatttgagtg cacagggaag cattcaccat
     1381 atcaaaggag attatgaaaa agctcagact ttttttcaga aatctctcaa aatccgcaag
     1441 cagatcggtc tggaaatgag tgcaggttat gcgaacatcc tttatctttc ctcaattgct
     1501 gatttcagaa tgggaaaatc ctgcgaagca aagacaaaag tcactaaggt cattgaaatt
     1561 tacagatatc ttggcgactc tgctgaagca aatcagatcg aatctgaagg actcggaagt
     1621 ttcaaaaaag catgtgaagc aacgatggtt gccggtaact gattatttga tcctagtcac
     1681 attaaatccg atcaccttgg ccgcctcgtt tccggaaaaa tcagaagttc ttacctccag
     1741 aacattctct ccggaaacaa aactcagatt tcctacaaag taatttccca gaaaaaacag
     1801 ctcttcaaaa ctctgaccag tctcattcac ccaggtattt tctctcagac tgatttcatt
     1861 aaatcctgca tccttcaaaa cttttccgtt cagaagaaat ttgactctct gaatccctct
     1921 cctctgtcct ggacggattc ctgagtccag aataggtatg gaaacaggga aggctctgga
     1981 aacattaata ttgtctccgt catttacgta cgtaaattta tccacattcg tcagaatcat
     2041 catcccgatc ttcggagcac ttgtatccgg tatgacagga agaaacggaa gaggattgat
     2101 gatttttttc cctccgtcct ttgccagaat aaaatgaaga tgagagccca tggagtgac
//
LOCUS       DAOQGJ010000580         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34127_length_2158_cov_4.653828, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000580 DAOQGJ010000000
VERSION     DAOQGJ010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34127_length_2158_cov_4.653828"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="PK683_09765"
     CDS             <1..1456
                     /locus_tag="PK683_09765"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH08778.1"
                     /translation="YFFLSRPRRFGKSSFLDTIQEAYQGNEVLFKGLFLEKNWDWSKK
                     YPVIKISFGSGETTSLEELRLIIHDIFLAFQRQFQITLESSSFSGKFKELIEILHKRE
                     NRKVVILIDEYDKPILDAIDKEFAYQVRDELKNLYSVIKDSDRYIQLVFITGVSKFSR
                     VSLFSGLNNLTDITLDPEFSAVCGYTQKELEDTFSELLENVPLREVKEWYNGYNFLGE
                     KVYNPYDILHYLRNKDFRNYWFETGTPSFLIKLIEKRKFPIPSLEKVELSKEMMDSFD
                     IGSIHLETILLQAGYMTIQSVEKLGDMTVYHLCYPNKEVRNSLNDVILTFLSSLVSEK
                     EKNKSRLYRILFAGEVEKLKDLFHSFFASIPYNWYTNNDIASYEGYYCSVFYTYFTAI
                     GVEVVTEDATNLGRIDMAVLLNGRCFIMEFKVNEMNPEGRAMKQLRDKKYHEKYTDYE
                     SQYLSSGKVKEIYLIGVEFNKTDRHITTFECVRV"
     gene            1463..>2158
                     /locus_tag="PK683_09770"
     CDS             1463..>2158
                     /locus_tag="PK683_09770"
                     /inference="COORDINATES: protein motif:HMM:NF020968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HNH08779.1"
                     /translation="MEHFSYPKISDNFQEVASRFQWTASEKIHGANTVIGTDGRTVRI
                     GKRKQWLEETEPFFGWQILRSFLEQSALSIFSSLKDAEILYLYGEIFGGEYPHPDVQN
                     IPSITAVQTGIWYSPDIRFALFDLLKVKNGKKIFLSFTEMEKLASEAGMLTVPKIASG
                     SLNELQSLPVRYISKMPSILGLPAIQNNYAEGFVLKPDAELNVSERPILKVKIPEFQE
                     NRFDESTAFNKDAM"
BASE COUNT          686 a          390 c          476 g          606 t
ORIGIN      
        1 atactttttt ctgagccgtc cccgccgatt cgggaaaagt tcttttctgg acacaattca
       61 ggaagcctat caaggcaatg aagttctgtt caagggtctt ttcctggaaa aaaactggga
      121 ctggtcaaag aaatatcctg ttattaaaat cagtttcggg agcggagaaa cgacgagcct
      181 ggaagagtta agactgatca tccatgatat ctttctggca ttccagagac agtttcaaat
      241 aactctggaa agttcttctt tttcgggtaa attcaaagag ctgatcgaga tactccacaa
      301 aagagaaaat cggaaagtgg tcattctgat agatgaatac gataagccga ttctggatgc
      361 aatcgacaaa gagtttgctt atcaggtgag agatgaactt aaaaatctgt attcagtgat
      421 caaggattcg gacagatata tacagctagt attcatcacg ggagtgagca aattttcacg
      481 agtgagcctg ttcagcggat tgaacaattt gactgatatt actttagatc ctgagttttc
      541 tgcagtatgc ggatatactc aaaaagaatt ggaagataca ttttcggaac ttctggaaaa
      601 cgtgccattg agggaagtaa aagaatggta taatggctat aactttttgg gagaaaaagt
      661 atataatcct tatgatatat tgcactactt aagaaacaag gatttcagaa actattggtt
      721 tgaaacaggc actccttcct ttctgatcaa actgattgaa aaaagaaaat ttcccatacc
      781 cagtctggaa aaagtggagc tgtctaaaga aatgatggac agttttgata tcggctccat
      841 tcatctggaa accattcttc ttcaggcggg ttatatgaca attcaaagtg ttgagaagct
      901 gggagatatg acagtctatc atctctgtta tccgaacaag gaagtcagga acagcctgaa
      961 cgatgtaatt ctgacttttt tatccagtct cgtttccgaa aaagaaaaga ataaaagcag
     1021 actgtatcgt atcttatttg caggagaagt ggaaaaactg aaagatctgt tccattcttt
     1081 ttttgcctcg atcccctaca attggtacac gaataatgat attgccagtt acgaaggata
     1141 ttactgttcc gtattctata cctatttcac tgccatagga gtcgaagtgg tcaccgagga
     1201 tgcaacgaat ctgggtcgca ttgatatggc tgttcttctg aatgggcggt gtttcataat
     1261 ggaatttaag gtcaatgaaa tgaatccaga gggaagagcg atgaaacagc ttcgggataa
     1321 aaaatatcac gaaaaatata cagattatga aagtcagtac ctcagttcgg gaaaagtaaa
     1381 agagatttat ctcatcggag tcgaattcaa taagaccgac agacatatta cgacttttga
     1441 atgtgtaaga gtgtaactca gagtggaaca tttcagttat cctaaaattt cggacaattt
     1501 tcaggaagtg gcttcccgtt ttcagtggac tgcttcagaa aaaattcacg gtgcaaatac
     1561 tgttatcgga acggacggca ggactgtgcg gatcggaaaa agaaaacagt ggctcgaaga
     1621 gacagaacct tttttcggat ggcagattct tcggagtttt ctggaacagt cagctctctc
     1681 cattttttcc agcctgaaag atgcagagat tctgtatctg tacggagaaa tttttggagg
     1741 agagtatcct catccggatg tacagaatat tccgtctatc accgctgtgc agaccgggat
     1801 ctggtattcg cctgatatcc gcttcgctct gtttgatctt ctgaaagtca aaaatgggaa
     1861 aaagattttt ctcagtttta ctgaaatgga aaaattggct tcagaagcag gaatgctcac
     1921 agttccgaaa attgcttcag gaagcctgaa cgaacttcag tcactcccgg tcagatacat
     1981 ttccaaaatg ccctccattc tgggacttcc tgcaatacag aataattatg cggaaggttt
     2041 tgtactgaag ccggatgcag aactgaacgt ttcagagaga cccatactca aagtgaaaat
     2101 tccagaattc caggaaaacc gttttgatga aagtactgca ttcaataagg atgcaatg
//
LOCUS       DAOQGJ010000581         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34134_length_2158_cov_3.674750, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000581 DAOQGJ010000000
VERSION     DAOQGJ010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34134_length_2158_cov_3.674750"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PK683_09775"
     CDS             <1..512
                     /locus_tag="PK683_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08780.1"
                     /translation="LHNAYHRVLKTVSLEDFNRLFASACAYLNDAVNSGHDFNPDKDR
                     TKENEVVLEQHDDSQQLIDKQTIYKGVYYMEISLKKDETRKAHEAVRIEVNDSYPQIT
                     KLGIQKNYINHYTADYNERQTWGHFIEILARTGRSTTFMNDRMRLTDRKKFMEAYSTV
                     MENVIKEVE"
     gene            516..1331
                     /locus_tag="PK683_09780"
     CDS             516..1331
                     /locus_tag="PK683_09780"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNH08781.1"
                     /translation="MFGAICGDVIGSYFERNNVKHKNFSLFSAKSRFTDDTVLTVAVA
                     DSIVNHRYFSSTIHSYSNLYPNAGYGSGFKKWFRSKHPHPYGSYGNGSAMRVSPVGFA
                     FNSLDEVLEISKKTSEISHNHPEAVKGAQAIASSIFLARQDRDKSEIRKFIEDSFQYD
                     LTKEIDEIRQDYKFDVSAQGSVPQAITAFLESTDYEDAVRNAISIGGDSDTIACMCGG
                     IAEAYYNKIPEEIIDSVLKIIPPDFTEIIKKFYSVYMKNSSIDFSSQKYTEPS"
     gene            complement(1300..1545)
                     /locus_tag="PK683_09785"
     CDS             complement(1300..1545)
                     /locus_tag="PK683_09785"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HNH08782.1"
                     /translation="MAGQQGPDPSIPLFGLKINKTAQDDPGKWEDKYAKCDTLLLSLE
                     NEDDHGLDFGGGYGNFLISSKDLKELKFKKVLYIFDC"
     gene            1721..>2158
                     /locus_tag="PK683_09790"
     CDS             1721..>2158
                     /locus_tag="PK683_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08783.1"
                     /translation="MKNSKTALNRSLGILCTLLSVMYSSMFCSLSDAVKKTQTDRNIS
                     RIVPGVPEETKAARIYEDGRMEVLKINPDSSWTHSGKSKNAVSPDGRYIAYSKDNNSF
                     WLFDADSGEDIPIHPKLNDYGYFTSEGWSVNNDKLMYKLEKNSK"
BASE COUNT          715 a          404 c          454 g          585 t
ORIGIN      
        1 gtctgcacaa tgcctatcac agagttttaa agacggtcag tctggaagat tttaacagac
       61 tgtttgcttc tgcatgcgca tatctgaatg atgctgtgaa ttcaggtcat gattttaatc
      121 cggacaagga caggacaaaa gaaaatgaag ttgtccttga acagcatgac gacagtcagc
      181 agctgattga taaacagact atttataaag gagtatatta tatggaaatc agcttgaaaa
      241 aagatgaaac ccggaaagcg cacgaggctg tcagaattga agtcaatgat tcttacccgc
      301 agatcacaaa gctcggaatt cagaagaatt atatcaatca ttacacagca gattataacg
      361 agagacagac ttggggacat tttattgaga ttttggccag aacaggacga agcacaacgt
      421 tcatgaatga cagaatgaga cttacagaca ggaaaaaatt tatggaagca tacagcactg
      481 tcatggaaaa tgtcataaag gaagtcgaat aaattatgtt tggtgcaatt tgcggagatg
      541 tcatagggtc ctatttcgag agaaacaatg taaaacataa aaacttcagt ctgttcagtg
      601 caaaaagcag attcactgac gatacagtgc tgactgttgc agttgctgac agcattgtaa
      661 atcacagata tttttcctca accattcaca gttacagcaa tttatatccg aatgcagggt
      721 atggatcggg attcaaaaaa tggttcagat caaagcatcc gcatccttac ggaagttacg
      781 gaaacggatc agcgatgaga gtgagtccag tcggttttgc ttttaacagc ctggatgaag
      841 ttctggaaat ttccaagaag acttctgaaa tctctcacaa tcatccggaa gcagtaaagg
      901 gcgctcaggc cattgcctct tctattttct tagcgaggca ggacagggac aagtctgaaa
      961 ttagaaaatt tattgaagac agttttcagt atgatttgac aaaggaaatt gatgagatca
     1021 gacaggatta caaatttgat gtcagtgctc agggttcagt tcctcaggcg attactgcat
     1081 tcctggaatc gacagactat gaagatgcag tcagaaatgc aatttccatc ggaggcgaca
     1141 gtgatacaat cgcctgtatg tgcgggggaa tagcagaagc gtattataat aaaattcccg
     1201 aggaaatcat tgactccgtt cttaagatta ttccgcctga ctttactgaa attataaaga
     1261 aattctattc agtttatatg aagaacagca gtattgattt cagcagtcaa aaatatacag
     1321 aaccttcttg aatttcagct ccttcaagtc cttgctgctg attagaaagt tcccgtatcc
     1381 tccgccgaag tccagtccat gatcatcttc attttcaaga gacagcagaa gtgtatcaca
     1441 ttttgcatat ttatcttccc attttccagg atcatcctgt gcagttttat ttatcttcag
     1501 gccgaaaagc ggaattgacg ggtcaggtcc ctgttgaccg gccaattaaa tccgagatcc
     1561 attgactttc aagaaaaata taatccatca gtatccttct atggaaaagt atattgaagc
     1621 ttccggcaaa gtcttatggg gataatactt gcattaaaat tcgagaatat aaaactgctt
     1681 atatttgatt cagttaaaaa tttcattcat ggatttaaag atgaaaaact caaaaacggc
     1741 attaaaccgg tcccttggca tactctgcac actgctctca gtcatgtatt catccatgtt
     1801 ctgttcactc agtgacgcag taaaaaaaac acagacagac agaaacattt caaggattgt
     1861 tccgggagtg ccggaagaga caaaggcagc ccgaatttat gaagacggcc gaatggaagt
     1921 tctgaaaatt aatcctgaca gcagctggac acactccggt aaaagtaaaa atgcagtctc
     1981 ccccgacggg cgttatattg catactcaaa ggacaataat tctttctggc tatttgatgc
     2041 agattcagga gaggatatcc cgattcatcc caagctgaat gattacggtt attttacttc
     2101 ggagggatgg tcggtgaata atgataaatt aatgtataaa ctggaaaaaa acagtaaa
//
LOCUS       DAOQGJ010000582         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34171_length_2157_cov_2.814938, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000582 DAOQGJ010000000
VERSION     DAOQGJ010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34171_length_2157_cov_2.814938"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PK683_09795"
     CDS             <1..703
                     /locus_tag="PK683_09795"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNH08784.1"
                     /translation="SLKGLVLLGLFFLGMTASVLTALIMKRTFFRNDPSYFIQELPFF
                     RFPSVKSTFLDLYGKIKEFIINTGMIIIYASAILWFLLNFPNEKKNFQPDSAAREEIT
                     LNSYAAAVGKTVEPVIRPLGFDWKIGISILGAFSAREVMVSTLAVIYGTDEKNEKQLS
                     SIMKEQKREDGSALWSMATGCSLLVFFAFACQCMSTLAVVRKETNSYFWTGMLFLYMT
                     VLAYISSFLTYQILS"
     gene            complement(669..1919)
                     /locus_tag="PK683_09800"
     CDS             complement(669..1919)
                     /locus_tag="PK683_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08785.1"
                     /translation="MMKFSKIIILPLFFFSCTKKQLPAPEPQNQSNAVIFELEVLRVM
                     DDRLPGLNDEDMKEILAEAERILKAKLGNESKFKFKDNGTAAAKDFFSGRNYKNSNSY
                     KENRNWKLNIQSNKKEIRKYLSQQKILKNTSHFLKDWSLDSLNQFFPGSKTYDQAAKN
                     LNAVYSEKIEWLKSLKVEEKDSLVILPPEDFQSYVEWLTLMFEQNQYDIIFTNSLIIN
                     DYMASPYPHTISKHAKIGGSSFISPKRSAMAGASLLVSTLEDFGRIKGLSSPLEIERQ
                     ERNRIIGGFLLAHEIGHAFYKIPDVYDHTDSCLMNSKWEKLNSVDGYRLLASDMSPCP
                     YCKKWEQSKNLYIDGLELQMRNDQLEAGKAFLKSAEILPERIDGDRKAYYNFLMTNAE
                     TALKKSGRDIQDFIRKESGTSEKN"
BASE COUNT          558 a          448 c          444 g          707 t
ORIGIN      
        1 cagcctgaaa ggacttgttc ttctcggact tttctttctt ggaatgactg catctgttct
       61 tactgccctg atcatgaaaa gaactttctt cagaaatgat ccttcttatt tcatccagga
      121 gctgcctttt ttcagatttc cttcggtgaa aagcacattc cttgacctgt acggaaagat
      181 aaaagagttt attataaata ctggtatgat aatcatctat gcttccgcaa tactgtggtt
      241 tcttctgaat tttccgaatg aaaaaaagaa ttttcagcct gacagtgcgg caagagagga
      301 aatcactctg aactcatatg ctgctgcagt gggaaagaca gtggaacctg tgattcgccc
      361 tcttggattc gactggaaaa tcggaatttc catcctgggt gctttttctg ccagagaagt
      421 catggtatcc acgcttgcag tcatatatgg aacggatgaa aagaatgaaa agcagctttc
      481 atcaataatg aaggaacaga aaagagaaga cggttctgct ctctggagta tggctaccgg
      541 atgcagtctt ttggttttct ttgcatttgc atgtcagtgt atgtccacat tggccgttgt
      601 caggaaggaa acgaattcct acttctggac aggaatgctc tttctataca tgactgtgct
      661 ggcttatatc agttcttttc tgacgtacca gattctttcc tgatgaaatc ctgaatgtct
      721 ctgccggatt tcttcagtgc agtctctgca ttggtcatca ggaaattata atatgccttt
      781 cgatctccgt caattctttc cggaagaatt tcagcagatt tcagaaaggc ctttcctgcc
      841 tccagctgat cattcctcat ctgaagttcc agaccgtcga tgtacagatt cttggactgc
      901 tcccattttt tgcagtaagg gcagggagac atatcggaag ccagaagcct gtatccgtcg
      961 acagagttca gtttttccca ttttgaattc atgagacagg aatcagtatg atcgtacaca
     1021 tcaggaattt tataaaatgc atgaccaatt tcatgtgcca gcaggaagcc tccgatgatt
     1081 ctgttccttt cctgtctttc tatctccaga ggactgctca gtccctttat ccggccaaag
     1141 tcttccagtg tgctgaccag cagacttgca cctgccattg cgcttctttt aggactgatg
     1201 aaagatgatc cgccgatttt tgcatgcttg gaaatcgtat ggggataggg ggaggccata
     1261 tagtcattta taataagact gttggtaaaa ataatatcgt actgattctg ctcaaacata
     1321 agtgtcagcc attccacata tgactgaaag tcttccggag gaagaatgac aaggctgtcc
     1381 ttttcttcca ctttcagtga tttcagccat tcaatttttt ctgaatatac agcgttgaga
     1441 ttttttgccg cctgatcata ggttttagat ccggggaaaa actgattcag tgagtcaagc
     1501 gaccagtctt tcaggaaatg ggaggtgttt ttcagaattt tctgctgaga taaatatttt
     1561 cggatttcct ttttattgga ctgaatgttc agtttccagt tcctgttttc tttataggaa
     1621 ttagagtttt tgtagttcct gcccgaaaaa aaatcctttg ccgctgctgt accgttgtct
     1681 ttgaatttga atttagactc atttccgagc ttcgctttca aaatccgctc tgcctctgca
     1741 aggatttctt tcatatcctc atcatttaat cctggaagtc tgtcatccat gactctcagc
     1801 acttccagtt caaaaatgac agcatttgac tgattctgcg gttcgggtgc cggcagctgt
     1861 ttttttgtgc aggagaagaa gaacagcggc agtattataa tttttgaaaa tttcatcatt
     1921 gcatccagaa tttgtatttg ctgaaattct gaaatgtaat tagaggaaag ttttcacata
     1981 agtcaagttt ttttgctctt aaccgtcttt ttcttctttg caagcattcc gttcagtatg
     2041 tctgtgcata caatctctga aagaaatgca gtgtctgtat taaaattcat ttcctggaaa
     2101 atttccagtc tgttgtccac aatcagagaa gaaagaccat gtacggcaga ccaggcc
//
LOCUS       DAOQGJ010000583         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34201_length_2156_cov_2.647311, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000583 DAOQGJ010000000
VERSION     DAOQGJ010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34201_length_2156_cov_2.647311"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PK683_09805"
     CDS             <1..559
                     /locus_tag="PK683_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNH08786.1"
                     /translation="NAFACPGGYILVTKGALDLVDNEAELAGILSHEITHVTLQHSGK
                     FEGKRDSILEMIAYFMAPGGDIINELTEVSVNAMVGQFFEQGRKKEEEIESDQAGAMY
                     LSLAGYSVSGASSYLSKMSKAQGNEIVLKTHPPTGERISSLERFISQQGLNKSGKVNR
                     EEFQSKYRNFLENWKKLPPPVKAKE"
     gene            complement(591..1187)
                     /locus_tag="PK683_09810"
     CDS             complement(591..1187)
                     /locus_tag="PK683_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08787.1"
                     /translation="MIFIKNRIISFTIIKNLVILSIVYVFIAGSFPADSPKKTVPQPE
                     AKPKVKTAPVKKTEKKNNSDSRKTAAKESKKAECCPIGRKFLSMVELEEICFNEGSTE
                     FRFRLLMDGAMCSISDSIDLTDNLGNSYKVSRMDGIAECPSITKNPLNKVFTWTFEKV
                     QSSAEILNLTEDINAIPFTSGWVWWYWNDIDIRQCRRK"
     gene            complement(1245..1550)
                     /locus_tag="PK683_09815"
     CDS             complement(1245..1550)
                     /locus_tag="PK683_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08788.1"
                     /translation="MNKSEKNFEMKVGEKAFYEFDQHASIGRHGAYVISDKNVLSLLD
                     QESKFENPDRPKGTTGGDKAKGTFIFQAMSAGKTEVTVTQKFRSEIEKEEKFTVTVK"
     gene            1961..>2156
                     /locus_tag="PK683_09820"
     CDS             1961..>2156
                     /locus_tag="PK683_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08789.1"
                     /translation="MYGYFMISIPGIILILFLIHRYLKKNGKEKSILFYSGIFYIMLG
                     LPIIYSWFLYIAIGFILIGIL"
BASE COUNT          623 a          423 c          396 g          714 t
ORIGIN      
        1 caatgcattt gcctgtcccg gaggttacat tcttgtaaca aaaggagcat tggatttggt
       61 agacaatgag gcagaactgg caggcattct ttctcacgaa atcacccacg taactcttca
      121 gcattccgga aaatttgaag gcaaaagaga ctccatactg gaaatgatcg cttattttat
      181 ggctccgggc ggtgacatta tcaatgaact gactgaggtc tcggtcaatg caatggtcgg
      241 ccaattcttt gagcagggaa gaaaaaaaga agaagagatt gaatctgatc aggcaggtgc
      301 aatgtatctt tcgttggcag gatacagtgt cagcggagcc tcaagctatt taagtaaaat
      361 gagcaaggct caaggaaatg aaattgtact gaaaacccat cctcctactg gggaaagaat
      421 ttcttctttg gaaagattca tatcccagca gggcttgaat aaaagcggga aagtaaatcg
      481 ggaagaattt caatccaagt acagaaactt tctggaaaac tggaagaaac ttcctccacc
      541 cgtcaaagca aaagaataaa tttcattcct gggctggttt tccatcagcc ctacttcctt
      601 ctgcactgcc ttatatcaat atcattccag taccaccaga cccatcctga agtgaaagga
      661 attgcattta tatcctcagt cagattcagt atctcagcag aagactgaac tttttcaaaa
      721 gtccaggtaa atactttgtt taacggattt tttgtgatgg aggggcattc cgctataccg
      781 tccattcgag acactttgta ggaatttccc agattgtcag tgagatctat ggaatctgag
      841 atagagcaca ttgctccatc cataagaagt ctgaatctga actcagtaga accttcattg
      901 aagcagatct cttcaagttc caccattgac agaaacttcc tgcctatcgg acagcattcc
      961 gcctttttac tctctttggc tgcagtcttt ctagaatccg aattattctt tttttctgtt
     1021 tttttgacag gagcagtctt tactttaggc tttgcttccg gctgcggtac agttttctta
     1081 ggagaatctg caggaaaaga gccggcaata aacacataga ctatagataa tattactaaa
     1141 ttcttgataa ttgtaaaact gataattctg ttttttatga aaatcatatc tttagcatct
     1201 gtccgccgca gatcggaagc gggtcagatg caatttttca gtgattattt gacagtgaca
     1261 gtaaactttt cttctttttc tatctctgat ctgaattttt gagttacagt tacttccgtt
     1321 tttccggcac tcatcgcctg gaaaataaat gtccctttgg ctttgtctcc gccggtcgtc
     1381 ccctttgggc ggtctgggtt ttcaaatttt gattcctgat ctaataaaga caggacattc
     1441 ttatctgaaa ttacataagc tccatgtctt cctattgaag catgctggtc gaattcgtag
     1501 aatgcttttt ctccgacctt catttcgaaa tttttttctg atttattcat gtcaatcata
     1561 tttcttcggc tgcttttatc ctgagaaaaa agacaggaaa tcattccgaa cataagtaaa
     1621 actgttagaa gatttctttt tctcatctcg gatctccttt tttgaagttt gcctaatagt
     1681 aaggacagtt ttatttcagt caatctgttt ttttaaataa tataagaaaa atccattttt
     1741 taatcagtgc acatctttat ttgaaaaact ttgtacttcc ttatatgatt tattcggaca
     1801 tcctaaccgg aaaatctgca tccaggttct gaaaaccttg caggaaaaat tacggctgtg
     1861 cggatcattt catcatcaga tcaagatcca tgttaaataa aatatttcct ttttttatcc
     1921 tgaatggaaa atgactctga cattcattcg aggataattt atgtatggtt attttatgat
     1981 ttccattcca ggaatcattc tgattttatt tctaatccac aggtatctga aaaaaaatgg
     2041 aaaggaaaaa agtattcttt tttattccgg aatattctat attatgttgg ggcttcctat
     2101 tatatacagc tggtttcttt acatagcaat aggttttatt ctcatcggaa tcctcc
//
LOCUS       DAOQGJ010000584         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34213_length_2155_cov_3.335238, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000584 DAOQGJ010000000
VERSION     DAOQGJ010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34213_length_2155_cov_3.335238"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..591
                     /locus_tag="PK683_09825"
     CDS             76..591
                     /locus_tag="PK683_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08790.1"
                     /translation="MIPFAKSLPHGIEDTLPLLPQLPRFNWKIFYLIFLIALIVWLVM
                     YFRKTQKSKPVLHYLKKFGKDFQVGSEMDDAFRRHTKKETFRAGLDELSDILKAYLEQ
                     RFNSSFEELTSEEIGRILNAPEFFRYLKDLDAKRFGSDSPDENEFRDMFEAARKQLKQ
                     ERNIKMLQGKI"
     gene            588..1595
                     /locus_tag="PK683_09830"
     CDS             588..1595
                     /locus_tag="PK683_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH08791.1"
                     /translation="MKTFHFGQPYFLYFLLLVPLYIFFRYRMLSLSAVQTVSAPEERK
                     THISKYGRLTVFLMIETFLLLSAGFGAADPYYAVLKTKVSEDGIDLALVLDVSASMQA
                     ADFSPNRLEKMKETAKDFIRNTPGARTGIFIFAQECFTQTPLTNDRNSLLNFTDSISY
                     YSIDHNESGGTAIGVALLSAAETLLRMKIPKRDQAVILITDGENSYGIDPVTVAKYLK
                     ENSIRLYIIGMAGPNPIKVYVNGSPFITPAGTVLETSLDDESLRKISDAAGGKYFRAH
                     DGEKFSEILKELSRMERTPLEVGEIVEKYSFADKAGAAVMLFLLLRLLFSHYFFRRPV
                     K"
     gene            1592..>2155
                     /locus_tag="PK683_09835"
     CDS             1592..>2155
                     /locus_tag="PK683_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH08792.1"
                     /translation="MNQDILSPLWLTVMIPASIVLFYIYIQNRVSLVWIEKNIHSRFR
                     PKFTVLNGKSLILYFGFLFLLLLLGAAAASNPGMKDTEIIEKEGQGTLIIVLDASFSM
                     MATDTVKNPTTKTVPVDRYDQAKDFANELVDRLPGWKFGLITFSGTAELELLPTNDLI
                     AVKTAILHSKVHNFEHTGTSFLSAFRIL"
BASE COUNT          596 a          455 c          482 g          622 t
ORIGIN      
        1 cgaaagtttt tctactgcag attctgtttc cggaaagctg ttccggtact tccatgccaa
       61 gagaaagaga agaatatgat tccttttgcg aaaagccttc ctcacgggat tgaagacact
      121 cttcctcttc tgcctcagct tcccaggttc aactggaaga ttttttatct tatttttcta
      181 attgcactga ttgtatggct tgttatgtat ttcagaaaaa cccagaagtc caagcctgtt
      241 ctgcattatc tgaaaaaatt cggaaaggac tttcaggtcg gttcagaaat ggatgatgct
      301 ttcagaaggc atacgaaaaa agagacattc cgggccggtc tggatgaact ttccgacata
      361 ctgaaagctt atctggaaca gagattcaat tccagctttg aagaactgac ttccgaggaa
      421 atcggcagaa ttctgaatgc tcctgaattt ttcagatatc tgaaagatct ggatgcaaaa
      481 agattcggtt cggattcccc tgatgaaaac gaattcagag atatgttcga ggcagcaaga
      541 aaacagctga aacaggaaag gaatattaag atgcttcagg ggaaaatatg aagacgtttc
      601 attttgggca gccttatttt ctttattttc ttcttcttgt tcctctgtac atttttttca
      661 gatacagaat gctttccctt tctgcagttc agacagtatc agctcctgag gaaagaaaga
      721 ctcatatctc aaaatatgga agactgactg tatttttaat gatagaaaca tttctgcttc
      781 tgtctgccgg cttcggtgcg gcagatccgt actatgcggt tctcaaaacg aaagtgtctg
      841 aggatgggat tgatctggct cttgttctgg atgtttctgc aagtatgcag gcagccgact
      901 tcagtccgaa cagactggaa aagatgaagg aaactgcaaa ggatttcatc aggaacactc
      961 ctggtgcaag gaccgggatt ttcatatttg cacaggaatg ttttactcag actcctctga
     1021 caaatgacag aaattctctt ctgaatttta cggacagcat atcctattac agcatagacc
     1081 ataatgaaag cgggggtact gcaatcggtg tggctctctt aagcgctgct gaaacgcttc
     1141 tcaggatgaa gattccaaag agggatcagg ccgtgattct gattactgac ggagagaaca
     1201 gttacggaat tgatcctgtc actgtggcaa agtatcttaa ggaaaattca atacggcttt
     1261 acattatcgg gatggcagga ccgaacccga tcaaagtata tgtaaacggc tctcctttca
     1321 ttaccccggc aggcacagtg cttgagacct ctctcgatga cgaatcactc cggaagattt
     1381 ccgatgctgc aggcggaaag tattttcggg cgcatgacgg agaaaagttt tccgagatac
     1441 tgaaggagct cagtcgtatg gaaagaacgc ccctcgaagt gggagagatt gtggaaaaat
     1501 acagttttgc agacaaggca ggggctgcag tcatgctgtt ccttcttctc aggcttcttt
     1561 tctctcatta tttcttcagg aggcctgtaa aatgaatcag gatattcttt ctcctctctg
     1621 gcttactgta atgattcctg catcgattgt acttttttat atatacattc agaacagggt
     1681 gtctctcgtc tggattgaaa agaacattca ttccaggttc cgtcccaagt ttactgttct
     1741 gaacggaaaa tccctgatcc tgtatttcgg atttcttttt cttctcctcc ttctcggcgc
     1801 agctgcggca tctaatccag gaatgaagga tacggaaatc attgaaaagg aaggacaggg
     1861 cacactgatc attgtgctgg atgcctcctt cagtatgatg gccactgata ctgtgaaaaa
     1921 tccgaccacc aagaccgttc ctgtcgaccg ttatgatcag gcaaaggact ttgcaaatga
     1981 actcgtggac agacttcccg gctggaaatt cggactgatt acattcagcg gaacagcaga
     2041 actggaactt cttcccacaa atgatctgat tgcagtgaag actgcaattc tgcattccaa
     2101 ggtgcataat tttgaacaca ctggaaccag ttttctttct gctttcagga ttctt
//
LOCUS       DAOQGJ010000585         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34274_length_2153_cov_2.754051, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000585 DAOQGJ010000000
VERSION     DAOQGJ010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34274_length_2153_cov_2.754051"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..769
                     /locus_tag="PK683_09840"
     CDS             254..769
                     /locus_tag="PK683_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HNH08793.1"
                     /translation="MNSLKTNVPENNDNRKFRIPLSGTGVSTFLIPKEMMKGYAERLQ
                     LYKSKGDYLNYLISRFRVLLGYYIIHPSGLKTQYQTPYQDLHKENLRVKDEDWAELTV
                     LSASTGMSRCLLFIRLLQMDLQDWDSLLKKIGILYNHPFSSQRMWELLAVIGLDRGGA
                     VFIRGFYPRRL"
     gene            874..>2153
                     /locus_tag="PK683_09845"
     CDS             874..>2153
                     /locus_tag="PK683_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH08794.1"
                     /translation="MANNLAEVYKEVAEKYGDKGAFYSKDEKKAFQPTSYKQIYEMGV
                     ALAEALIDLGVKARENLGFMADHRMEWVVVDCGVLTAGAANVPRGTDVTETELEYILN
                     HSEAKVVFVEHDKMLEKLNKVKSKTSIKTIIVMDKNSKAEGVLKLYDLIEKGRQLIAK
                     GSKKYEERVKDIKSDDLFTMIYTSGTTGQPKGVMLMHSNMMHQVNDVVPVINIGPNER
                     LLSILPVWHIFERVFMYGAIVAGAGTYFTNVRDMREDFQKVKPTFMASAPRLWESIYQ
                     GIYNRINDPKQTPPFRRGMFNTAYFFSKHFHAAVRFIKGNEVDFEGRNPLTSLFKGIY
                     SVIVMILTFIPYAILDAVVLSKIRTATGGSMKATVSGGGALQRHVDAFFNDIGLNVIE
                     GYGMTETSPVISMRTWETLIMGSVGRVAPKTELQ"
BASE COUNT          695 a          395 c          462 g          601 t
ORIGIN      
        1 gcagctgatt acgagtaaaa tgagtgcgac tgccgttctg taaatcataa ctgtatttcc
       61 gtcctgttct gcctgaatag taagacagga atagaatgaa tccttatacg gcaagaataa
      121 aatcctctga aaaatgtagg aactcctatt atctgtatta ttcaaaaatg cgtatttgat
      181 taaaaagaac ttaaagggtg cataaagctt aatttttttc gagcatattg attttttcgg
      241 gacaggtata tttatgaaca gcctgaaaac caatgtaccg gaaaataatg ataatcgtaa
      301 atttcgtatt cctctttcag gaaccggtgt atcaactttt ctcattccca aagaaatgat
      361 gaaaggatat gccgaaagac ttcagctgta caaaagcaaa ggggattacc taaattatct
      421 catttccaga ttcagagtac tgcttggata ctatatcatt catccatccg gtctaaaaac
      481 gcagtatcag actccttatc aggatttgca taaagaaaat ttgagagtga aagatgagga
      541 ttgggctgaa ctgactgtct tatctgcatc aaccggtatg agccgctgtc ttctcttcat
      601 ccgtctcctc caaatggatc ttcaggattg ggattctctc cttaaaaaaa tcggcattct
      661 ttataatcac cccttctcct cccaaagaat gtgggaactc cttgccgtga ttgggctgga
      721 tagaggaggg gcagtgttca tcagaggatt ttatccaaga aggctgtagc aggagtaaaa
      781 aaataaaaaa atttgttgca tttttgccgg ataaaaagaa cttcagaacg gcacacatct
      841 atataataca attattcaaa ggagagcgtg taaatggcta acaaccttgc ggaagtgtac
      901 aaagaagtag cagaaaaata tggggacaaa ggcgcttttt actcaaaaga tgagaaaaaa
      961 gcatttcagc ctacttctta caaacaaatt tatgagatgg gggtagctct cgcagaagcg
     1021 ctgatagacc ttggtgtaaa agcccgtgaa aatctcggtt ttatggcaga ccacagaatg
     1081 gaatgggtag ttgtggactg cggtgttttg acagcaggag ctgcaaacgt tccgcgcggt
     1141 acagatgtga ccgaaactga acttgaatat attctgaatc attcggaagc aaaagtagta
     1201 tttgtagagc atgataaaat gcttgaaaaa ctgaacaagg taaagtcaaa aacatccatc
     1261 aaaacaatca ttgttatgga taaaaactcc aaagcagagg gagttctaaa gctgtatgac
     1321 ttgatagaaa aaggccgtca gcttatcgca aaaggttcga aaaaatacga agaaagagtc
     1381 aaggatatca aatccgacga tctgtttaca atgatttata cttccgggac aaccggccag
     1441 ccaaagggag ttatgctgat gcattctaat atgatgcatc aagtgaatga tgttgtgccg
     1501 gtgatcaata taggtccaaa tgaaagactg ctttccattc tgcctgtctg gcatattttt
     1561 gagagagtat ttatgtatgg ggcgattgtt gcgggcgcag gcacatattt tacgaatgtc
     1621 cgtgatatga gggaggattt ccagaaagta aaacctacct ttatggcatc tgctcctcgt
     1681 ctctgggaaa gcatttacca gggaatttat aacagaatca atgatcctaa gcagactcct
     1741 ccgttccgaa ggggaatgtt caacactgct tatttcttct caaaacattt tcatgctgca
     1801 gtccgtttca tcaaaggaaa cgaggttgat tttgaaggaa gaaatcctct cacttcactt
     1861 ttcaaaggaa tctattcagt tatcgtaatg attctcacat tcatccctta tgcaattttg
     1921 gatgctgtag tcctttctaa aatcagaact gcaactggcg gatcaatgaa agcaacagtt
     1981 tccggaggcg gtgcacttca gagacatgtg gatgcattct tcaatgatat tggactgaac
     2041 gtaattgaag gttacggaat gacagaaact tcacctgtga tttcaatgcg aacttgggaa
     2101 acactgatta tgggttcagt tggaagagtt gctccaaaaa cagaacttca gtt
//
LOCUS       DAOQGJ010000586         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34276_length_2153_cov_2.662536, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000586 DAOQGJ010000000
VERSION     DAOQGJ010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34276_length_2153_cov_2.662536"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..372)
                     /locus_tag="PK683_09850"
                     /pseudo
     CDS             complement(1..372)
                     /locus_tag="PK683_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
     gene            complement(904..1212)
                     /locus_tag="PK683_09855"
     CDS             complement(904..1212)
                     /locus_tag="PK683_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08795.1"
                     /translation="MQDNLEKLKKENEEMKLIIKGYEKVLRLNEKELSNADEIIKMYE
                     SILSYASSELKNAQETMKASDIVSNISREELMEALVRIKELELQNRKLREESLKLNLK
                     "
     gene            1278..1350
                     /locus_tag="PK683_09860"
     tRNA            1278..1350
                     /locus_tag="PK683_09860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1311..1313,aa:Ala,seq:ggc)
     gene            1540..2118
                     /locus_tag="PK683_09865"
     CDS             1540..2118
                     /locus_tag="PK683_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08796.1"
                     /translation="MIHYFHSWAGKEFLVQDSEALQKTLEKMESVITSVPRKTCIIAR
                     GYSAAAALFLANSISFKLNNIILLNPVLLFQRQKTKEPHMLHFTKWFFEDKDPFWDMF
                     EAPPYLEFFNNYLDWVKNISQMEQTMQMACANLESGYIRDPWLFANVHPEIKVYTWDR
                     ALKNDPLGNPESRKFLYFIYKKELERNTAKKD"
BASE COUNT          575 a          479 c          423 g          676 t
ORIGIN      
        1 gagcggagta gcaataatgt ttcttgtgac aaatgcggat gccttgtcca ccaccttgta
       61 caccctgtca tcattctgag catcattgat gatcatcggt tctctggtga ctgccactgt
      121 tcctgcaatt ccttttccca tcggaacccg gattttggag atttcttcct ctttctctcc
      181 agccactata tgaaaataga gttcattttt ggccttgtcc acaaggagta tggaacagga
      241 ttctgtgcgg aatacagttt tgaccgattc catcacctca aggagcagtg ctttcagatt
      301 ctttggagag ttgatgagac cggatacccg gataatttcc tgaagcagat attccagtct
      361 gttgtttttc tggtaattct ctgtagaatg caggaggagc aggagtgatg aggagaatcg
      421 aagataaatc tcttcctgtt ccgcagcata agacgattca cagaaaagca gtacgagcgt
      481 cttggaatac acttcagcca gttcgaaatc agagatggaa aaattttcaa agtggttcag
      541 tccagaaagt acaatgactc ccacatccag aaaaccgtcc ggatctccta aaaaacagac
      601 aatatgagat tctgcaattt cccgtccctc aaggacttct ttctttttta ccagtcttcc
      661 ttttctttct cggaggccaa gttctgccag aaaccttgtt tcttccgtat tacttcctac
      721 aatggcatcc ttcaaagtcc cttctgaatc cagaagatac agcacctcgt tctgtgctcc
      781 cacaagattt gcaatctctg agagaatcag tgaaagattg tcttccagct tttccagtct
      841 gggaaaataa gcggttgctc ctttcccctt tttcagaagt gattgaaggc tgtctgctga
      901 cacttatttc agattgagtt tcagggactc ttccctcagt tttctgttct gaagctccag
      961 ctccttgatc cgcactaaag cttccatcag ttcttcacgg gaaatattgg acacgatatc
     1021 ggaggctttc attgtttcct gagcgttttt cagttcactg gaagcataag acagaataga
     1081 ttcatacatc ttaatgattt catctgcatt agacagttcc ttttcattga gacgaagcac
     1141 cttttcgtat ccttttataa tgagtttcat ttcctcattt tctttcttca gcttttccag
     1201 attatcctgc atatcctctt tcctccaagg aataattgtg tttgactcga caaacctgag
     1261 ttaaatattc gtctcagggg gacgtagctc agttgggaga gtgtttgaat ggcattcaaa
     1321 aggtcggggg ttcgattccc ctcgtctcca tatcttccct aaaaaagcat aattctatat
     1381 tccctcatga tttcaagcaa aatcaatcat aaagacatcc tggaatttga aacttttggg
     1441 gaaacctctg gttcttcagt tgtatttctg gacgatttct ttcaacctga aaaaagatct
     1501 gcagtccgtt ttttaaggga ctttctcgca gaaaattcaa tgattcatta ctttcattcc
     1561 tgggcgggca aggagtttct tgtacaggat tcggaggccc ttcagaaaac tctcgagaaa
     1621 atggaatctg tgatcacttc tgttccgcga aaaacctgca tcatagccag gggatattct
     1681 gcggcggcgg cactttttct ggcaaattcc atttctttta aactgaacaa tatcattctg
     1741 ctgaaccctg ttcttctttt tcagaggcag aagacaaaag agccgcatat gcttcatttt
     1801 acgaaatggt tttttgagga caaagatcct ttctgggata tgtttgaagc cccgccttac
     1861 ctggagtttt tcaataatta tctcgactgg gtaaaaaaca tttcccagat ggagcagaca
     1921 atgcagatgg cctgcgcaaa tctggaatca ggctatatcc gcgacccttg gctttttgcg
     1981 aatgttcatc cggaaattaa agtttatacc tgggacaggg ctctcaaaaa tgaccctctt
     2041 ggaaatcctg aaagcagaaa gtttttatac tttatatata aaaaagaatt agaaagaaat
     2101 acagcaaaaa aagactgatt ttgcaagttt catctgtttc atcagatcgg ctc
//
LOCUS       DAOQGJ010000587         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34298_length_2152_cov_2.999523, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000587 DAOQGJ010000000
VERSION     DAOQGJ010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34298_length_2152_cov_2.999523"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1429
                     /gene="thrC"
                     /locus_tag="PK683_09870"
     CDS             104..1429
                     /gene="thrC"
                     /locus_tag="PK683_09870"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNH08797.1"
                     /translation="MKYFAQFECINPECKSLYPLTEVIYKCRHCGEILQVSHNMEALK
                     QKSADEWKSLFDSRRGSFEFPNDSGIWSKREWVLPEIEPENIVSSGEGWTHLFNAERW
                     AKKLGLESLYVKQCGVSHTGSFKDLGMTVLVSQVNQLRKQGKNIRAVACASTGDTSAA
                     LASYAAKAGVPTIVFLPADKVSTAQLIQPVSNGAKVISMDTDFDGCMKIVQEVSEDSS
                     IYLANSMNSLRIEGQKSISVEILQQLRWKCPDWIVIPGGNLGNVSALGMGFEMMLELG
                     LIDRLPRIALAQSENANPLYQSFLKGFSDFQAVSAKPTLASAIQIGNPVSVKKAVRIL
                     KKFGGVVEQASEAELSDAAAEGDLYGLYNDPHTGVALGAMLKLLKKGTIEKGSKVTVI
                     STANGLKFTEFKVKYHEAGISGTNPDLKNTVHKLEAKTGAVMDLLKKII"
     gene            1507..>2152
                     /locus_tag="PK683_09875"
     CDS             1507..>2152
                     /locus_tag="PK683_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08798.1"
                     /translation="MESDLEEQYLTPDFRHAVKTAVIAGKRIHFQTFVLSESGEAKLK
                     HIIFSIIQKLDREDLMELIYTSAKELILNSIKAAIKRITLMEMGINPDDPNEYDHAMA
                     KFKENLTDKKFPYFKEKMKGQGYYVSVIFSYNSNRIIMKIINHFPLLTREELRIREKF
                     VNAKKYENLFEFYMDHGDNTEGAGMGITLVEIMMAQSGFDRRLFTIYTKHNPSST"
BASE COUNT          699 a          431 c          471 g          551 t
ORIGIN      
        1 aaatcagttc tccacgactt cctgtgaaaa aatctgatta tttttctcga tttcgatcag
       61 agtaaaaact tgtattgaaa agcagttcta aaactcttac actatgaaat actttgcaca
      121 gttcgaatgc atcaatcctg aatgtaagtc tctttatcct ctgactgagg tcatttacaa
      181 atgcagacat tgcggcgaaa tccttcaggt ttcccataat atggaagcac tgaagcagaa
      241 atctgcagac gaatggaaat ctctgtttga ttccagacgg ggttctttcg aatttccgaa
      301 tgattcaggg atctggagca aaagagaatg ggttctgcct gagattgaac cggaaaacat
      361 tgtcagttcc ggagaaggat ggacgcatct tttcaatgca gaacgctggg caaaaaaact
      421 cggactcgaa tccctgtatg taaaacaatg cggagtatct cataccggtt ccttcaagga
      481 tctgggaatg actgtgctcg tgtcacaggt caatcagctc aggaaacagg gaaaaaacat
      541 ccgtgcagtc gcctgtgcca gtacagggga tacctcagcg gccctcgcct cctacgcagc
      601 caaggcagga gttcctacaa tcgtctttct tccggcggac aaggtttcca cagctcagct
      661 cattcagccc gtatccaacg gtgccaaggt catctctatg gatacagatt ttgacggatg
      721 catgaaaatc gttcaggaag tttcagaaga cagttccatt tatctggcaa actcaatgaa
      781 ttctctcagg atcgaaggac agaaatccat atcggtagag atactgcagc agctccgttg
      841 gaaatgcccg gactggattg tgattcctgg aggaaatctg ggaaatgtat ccgcactggg
      901 aatgggcttt gaaatgatgc tggagctggg tctgatcgac agacttccta gaattgcact
      961 ggcacagtca gaaaatgcaa atcctctcta tcagtctttc ctgaaaggat tctctgattt
     1021 tcaggcagta tccgctaaac caactttggc atctgcaatt cagattggaa atccggtttc
     1081 agtgaaaaaa gcagtcagaa tactgaaaaa atttggaggt gtcgtagaac aggcaagtga
     1141 agcggagctt tcagatgcag cagccgaagg agatctgtac ggactttaca atgatccgca
     1201 tactggagtt gcactcgggg caatgcttaa gcttttgaaa aagggaacca tcgaaaaggg
     1261 atcaaaagtc actgtcattt ccactgcaaa cggtctaaag tttactgaat ttaaggtaaa
     1321 ataccatgaa gctggaattt caggtacaaa tcccgacctt aagaacactg ttcataaact
     1381 ggaagcaaag acaggagccg tcatggatct gttaaaaaaa attatatgag aattaaattt
     1441 atttacaaaa ataaatatgg cagcactatt gttttcgctt aaatgaaaca ggggaatgaa
     1501 agttaaatgg agtcggattt agaggaacaa tatcttacac ctgacttcag acatgctgtg
     1561 aagacagccg tcatcgcagg aaaaagaata cactttcaga cttttgtact gagcgaaagc
     1621 ggtgaagcga agctgaagca tattatattc agcattatcc agaaactcga cagagaagat
     1681 ctgatggagc tgatctatac ctccgcaaaa gaactcatac tcaactcaat caaggccgca
     1741 atcaaaagaa tcacattgat ggaaatgggc attaatcctg atgaccccaa tgaatatgac
     1801 catgcaatgg ctaaatttaa ggaaaacctt actgacaaaa agtttccata cttcaaagaa
     1861 aaaatgaaag gccaaggata ctatgtcagt gtcatatttt cctataattc caatcggatt
     1921 attatgaaaa tcatcaatca ttttcctctt ctcaccagag aggagctcag gattcgggaa
     1981 aaatttgtaa atgcaaaaaa atatgaaaat ctgtttgagt tttatatgga tcacggggac
     2041 aatactgaag gtgcaggtat gggtattact cttgtggaaa tcatgatggc gcaaagcggg
     2101 tttgacagaa ggctgtttac aatttatacc aagcacaatc caagctcaac ag
//
LOCUS       DAOQGJ010000588         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34410_length_2148_cov_2.476350, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000588 DAOQGJ010000000
VERSION     DAOQGJ010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34410_length_2148_cov_2.476350"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK683_09880"
     CDS             complement(<1..523)
                     /locus_tag="PK683_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08799.1"
                     /translation="MKFLLLAGVLYLAQAFGLNAQVNSPPYSPPDANLRPNPDTFSYD
                     VGVNKYDGDTYLYFSPSFNMNFSNKWGFSLQVPLNILMSDKDPKYPKSRVGMLRPGDY
                     NTREDYQRILNYVWLGKYGVYTPNKITYSVYAGKMFDGYIGHGTIINRYINNQRIDYY
                     KIGVQADINTDYGG"
     gene            complement(872..1270)
                     /locus_tag="PK683_09885"
     CDS             complement(872..1270)
                     /locus_tag="PK683_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNH08800.1"
                     /translation="MSRKNIDQLIKEFDKLRLHFKTPNYTKKQLEELEKNLKCKFPPD
                     FKKTLQAGYIDKGTFYFLPPERMKNNERFLIFGKWNDNVFMFDTEAGIKDMPVYVIVK
                     ETDLPEKCFESFYEWLNTVLKSVASAHFPG"
     gene            complement(1823..1978)
                     /locus_tag="PK683_09890"
     CDS             complement(1823..1978)
                     /locus_tag="PK683_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08801.1"
                     /translation="MGIARLDKVALGLGIALLQTYPEYKESDVILTVMTAAGFMIAVW
                     DFFKKDT"
BASE COUNT          646 a          429 c          428 g          645 t
ORIGIN      
        1 ccccgccgta atccgtattg atatccgcct gaactccgat tttataatag tcaattctct
       61 gattattgat gtaccggttg atgattgtac catgccctat gtaaccgtcg aacattttcc
      121 ctgcatagac ggaataagta attttattcg gcgtatagac tccgtacttt cccagccaga
      181 catagttcag aattctctga tagtcttctc gggtattgta gtcccctgga cggagcattc
      241 ccaccctgga tttaggatac ttgggatctt tgtcgctcat cagaatattg agaggaacct
      301 gcagggaaaa tccccattta ttgctgaaat tcatattaaa tgaaggagag aaatacagat
      361 aagtatcccc gtcatattta tttaccccga catcatagga aaatgtatca ggattcggtc
      421 tcagatttgc atcgggcggg gaatagggag gagaatttac ctgtgcattc agaccgaatg
      481 cctgtgccaa atacagaacc cctgccaaaa gtaaaaattt catctataat accttcttgg
      541 atcagataaa caggtacgaa cagacgatca aagtctttgc ctgattatat gaattttaac
      601 agagtttcat tctgtccaaa gcctgaaaaa aaacaatcag aaatcgaaga ggcggaagat
      661 taacctggga gcagagattt atccgaaaac gtgcttaagg aaaacgcgga tcagagaaaa
      721 caagtaaata caaaagctga gaatcgaatg gaaaaccgga aactcagcca gtttttatgg
      781 actgcataaa agcaaggtta aagagcggta ctgtgagtct gctgaacttt gcttttgcac
      841 aatcctttgt gtgcagatca ataaagccgg attatcccgg aaaatgagct gaagccacag
      901 atttcaaaac tgtattcagc cattcataaa agctttcaaa gcatttttcc ggaagatccg
      961 tttctttgac aattacatag acaggcatat ccttgattcc agcttctgta tcaaacataa
     1021 aaacattatc attccatttt ccgaaaatca ggaatctttc attgtttttc attctttccg
     1081 gcggaagaaa ataaaatgtt cccttgtcta tataaccggc ctgcagggtt tttttgaaat
     1141 caggagggaa tttgcatttt aaattttttt ccaattcctc cagctgtttt tttgtgtaat
     1201 tcggagtctt aaagtgaagt cttaatttat cgaattcttt aatcagctga tcaatattct
     1261 ttctgctcat atccaacctc ttaaagtcta gtctaaaata atatccattc cttgccacaa
     1321 cttttatgct caaattcctt attaaatttt ctgtgattat cactgtattc gttttatgca
     1381 atctgatatc attccgagaa atgtgctgtc aatgcggtca aaggttttga aaactcagta
     1441 cattgcactg tatgtatagt gagacttaaa atccatgatc tatgtgaatg catattcgaa
     1501 tctttgcaga aatggaaacg gtttagcgtt ctgtctgaaa ctgtccggga tatatgccgg
     1561 aaataaagtg caatatataa aatttaatta tcattaatca gatcaaatcg caagccggag
     1621 aaaaagttct gatctggact gagtttccgt tcagggcagg ggaaaaggaa tattgccggt
     1681 attctttttt ctttatatta tactgtatac ctcgcttcgc tgccaggcga ggttttacca
     1741 gctttctcga tcaagaatgg tgaattgatg tcgctgatca aaaggaatca gtcctgatgt
     1801 gcaatgacat atcgaaagcc ggttatgtat ctttcttgaa aaaatcccag actgcgatca
     1861 taaatcctgc agccgtcata acagtgagaa taacatcgct ttctttgtat tcaggatatg
     1921 tctgaagaag tgcgattccc aatcctaatg cgactttgtc aagtcgcgct attcccatct
     1981 gcagccaatt atcacatccc cgacgactaa aagtcggcgg ggaaggggaa aacgcccgca
     2041 agcttggaag tcgctgccgg cggggctggg gttgtgtaaa agctcggata ctgtagaaaa
     2101 atgattgact ttgatgcatc tgcagtatcc ggcagccggt ttcaaatt
//
LOCUS       DAOQGJ010000589         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34451_length_2146_cov_3.585844, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000589 DAOQGJ010000000
VERSION     DAOQGJ010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34451_length_2146_cov_3.585844"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK683_09895"
     CDS             <1..185
                     /locus_tag="PK683_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08802.1"
                     /translation="DITYYILKMMSWVGLVWDLKPVPAHVLDEGKRRDALAKGKETFK
                     DHSGQLISVNANIHPI"
     gene            complement(287..961)
                     /locus_tag="PK683_09900"
     CDS             complement(287..961)
                     /locus_tag="PK683_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08803.1"
                     /translation="MKTGIITENCRQEAVFANMKGIIVSEEDAKRDGFYSGSGLFSHP
                     LIQKIWPDFSSRFRSKKNPLKKIIAWFSYPYNFEDWGMNHYFYHLGVFALKNGKYTYI
                     HAGGASPDLCPEMPWIYFFETETLEELAEKIPQEAAIVLRLNMTAQQIENSSKKEKEY
                     IRDLVLYSVRWPKLYMNCARYDSWNGVFSPVIPVTEKEIEDQEEAFLKRKTPVQKERK
                     IKSKNK"
     gene            complement(1106..1939)
                     /locus_tag="PK683_09905"
     CDS             complement(1106..1939)
                     /locus_tag="PK683_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08804.1"
                     /translation="MKKIILSILIFSFISCAGFNRKNLLLATAVEENLVPEKPPMRYI
                     AAPFYLPLGIAGGFLDVFLIHPLRSVPRAADDTIDALWRNREGGYITKMGAVPFLTVA
                     SPVFFAAVWMVRSFFDVGSSDYKPAEQSGSEEAFATAEKNFQENKKAELTESLYAVCY
                     SGASYPDSFVRLLQNSYDRYKADANFSSTVISCMAQEKLYSKSEEFLFSKFEEEDNNQ
                     VFIMNTLASGKSKKRLSEAVLQKIFSKNSKKEYLQDYIMTLYRLENREHIQKFQNKLK
                     D"
     gene            complement(1936..>2146)
                     /locus_tag="PK683_09910"
     CDS             complement(1936..>2146)
                     /locus_tag="PK683_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08805.1"
                     /translation="PLGTVTLLADTFILHPVRVLPKSAEKTWELFWQNSEGGIVLQTF
                     LFFPKLILTPVGFVFIWLSYSIFDI"
BASE COUNT          646 a          462 c          397 g          641 t
ORIGIN      
        1 tcgacattac atattacata ctgaagatga tgagctgggt cggactcgtc tgggatctga
       61 aacctgttcc tgcacacgtt ctcgatgaag gaaaaagaag agatgctctg gcaaaaggaa
      121 aagaaacttt caaggatcat tccggacagc tgatcagtgt aaacgcaaac atacatccga
      181 tataatgaaa aaagcaagtc ttgcagaata cagcaagact tgctttttga gcatctaaat
      241 tcaatctata aacaatactc tcaaagaaat acagtcagaa cagctttcat ttattttttg
      301 attttatctt tcgttccttc tgaacaggag tttttctttt cagaaaagcc tcttcctgat
      361 cttcaatttc tttttcagtt acgggtatta ctggagaaaa aactccattc caggaatcgt
      421 accgtgcaca gttcatataa agcttaggcc agcgtacact gtacaaaaca agatcccgaa
      481 tatactcttt ttcttttttt gaactgtttt ctatctgctg tgcagtcata ttcaaacgga
      541 gaacaattgc tgcttcctgc ggaatttttt ccgccaattc ctccaaagtc tctgtttcaa
      601 aaaaataaat ccaaggcatt tccggacata ggtcaggcga ggctcctcct gcatgaatat
      661 aagtatattt accgtttttt aatgcaaata ctcccagatg ataaaaataa tgattcatcc
      721 cccagtcttc aaaattgtaa gggtaagaaa accaggcaat tattttcttc aaaggatttt
      781 ttttggagcg gaatctgctg ctgaaatccg gccatatttt ctgaattaat gggtggctga
      841 acagacctga tcctgaatag aaaccgtctc gttttgcatc ttcctcgctt acaataatgc
      901 ccttcatatt ggcaaatact gcttcctgcc gacagttctc tgttataatt cctgtcttca
      961 actgtgtctc tttttacctg ctgactgcct gagggaaggg aatcccgcag tcaaactgct
     1021 ggacttatcc aatgcttcgc agaaaatcca gattccaggc attatattct ttttgcacaa
     1081 ggtgagatcg aatataaggc cctgcctaat ccttcagctt attctgaaat ttctgaatat
     1141 gttccctgtt ttcaagacgg taaagagtca ttatataatc ctgaagatat tccttctttg
     1201 aattctttga aaaaatcttc tgaagaactg cttcactcag tcttttcttt gatttcccgg
     1261 atgctagagt attcataatg aaaacctgat tattgtcctc ttcttcaaat ttactgaaca
     1321 gaaactcttc cgattttgaa tacagcttct cctgtgccat acaggaaatg acagtggaag
     1381 aaaaatttgc gtcagcctta tacctgtcat aactattctg aaggagtcgg acaaaactgt
     1441 cagggtagga agctccggaa taacatacag cgtatagact ttcggtaagc tcagctttct
     1501 tattttcctg aaagtttttt tcagccgtgg caaatgcttc ctcgctccct gactgctctg
     1561 caggtttata atcactgctt cctacatcaa aaaaacttct gaccatccag actgccgcaa
     1621 aaaaaacagg agatgcgact gtcagaaaag gcacagcacc catttttgta atgtacccgc
     1681 cttcccggtt tctccaaaga gcatcaattg tatcatcggc ggctcttgga actgacctca
     1741 gtggatgaat cagaaataca tccagaaatc ctcctgcaat tccgagcgga agatagaaag
     1801 gagcagcaat gtatcgcatc ggaggttttt caggaaccag attttcttcc acagctgtcg
     1861 caagaagaag gttttttctg ttgaatcctg cacaggaaat gaacgaaaaa atcaatattg
     1921 ataaaataat ttttttcata tatcaaatat gctgtaactc agccatataa aaacaaaacc
     1981 gactggagta aggataagct ttggaaaaaa gagaaatgtc tgaaggacaa tccctccttc
     2041 agaattctgc cagaaaagct cccaggtttt ctctgccgat ttaggaagca ctctgaccgg
     2101 gtgaaggatg aaagtgtcag caagaagtgt gactgttcct aacggg
//
LOCUS       DAOQGJ010000590         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34500_length_2144_cov_3.583533, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000590 DAOQGJ010000000
VERSION     DAOQGJ010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34500_length_2144_cov_3.583533"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>2144
                     /locus_tag="PK683_09915"
     CDS             18..>2144
                     /locus_tag="PK683_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08806.1"
                     /translation="MRNPLIKYECDIFRTDEKYISHYELEKRVTVNIAYFGSGKTQSV
                     INHIKKTGQKIVFVHPTRALISDCISRLRQAGIEIKSHLELNQESALFDYDGNLEITL
                     ESLSKIDFSKYKDALFVFDEFVSIMNQMYSGYNSDRVQDMAKVLKHIFQNCKVCVFDS
                     LIQSFEIDFIYALSGENPGADIEILSNEFLTIKKRPLFRTEDKGFFEKNLIEAVKSGT
                     KVLFLTDDKSYSEFITSQIEDSAEVDTDSSTVSILSRDTRSELLDGRTIADFIREKKP
                     KLLSVTPTGFVGLDIPEGSFDMVYMYGGNFGIRDYRIYIQALHRLRDYSAPAVFYSNK
                     GTYDPMLLTSADSILVKRAESNNKLNDKIFYDLGKDGFWKPKEEYSAFENLSAELEAY
                     QNRTAKIGLAQYISDFFNERFGFETSILREKSESLERPSLKERTEEKKNRLKAVKLID
                     KQEYDSLRRNQAGRDLNKEETALVQKYEIAEKFSIGHTDIQRNEKVNLIIDYTANPDK
                     MRVIAENFEWLKNSDSVNYEKEKADYEKTSGIGQTAKSKTLETMLLKKILSSLDGKEF
                     GKTDIPMSLIYELETVTGRQFTASYNDAILDRKCFADLKGRLQYDALALDYAKSISEG
                     DENIIREVFKSDIMKIFDSEMGKLDKRKKRIQSRSQNPGLIQKHEEEASKKAAEAIKK
                     RVSKFIQSKRNDFAVSPLSILSDWLSN"
BASE COUNT          728 a          376 c          460 g          580 t
ORIGIN      
        1 gccgtcgggg gggcaaaatg agaaaccctc tgataaaata tgaatgtgat attttcagaa
       61 ccgatgaaaa atatatctct cattatgaac tcgaaaagcg ggtcactgta aatattgctt
      121 atttcggatc tggaaaaact cagtcagtca ttaatcacat aaaaaagaca ggtcaaaaaa
      181 ttgtatttgt tcatcctaca agggcattga tttcagactg catatccaga ctgagacagg
      241 caggaattga gataaaatca caccttgaac tgaatcagga atctgcactt tttgactatg
      301 acgggaatct ggaaattacc ctggagtcac tgagcaaaat tgatttttcg aaatacaaag
      361 atgctctttt cgtttttgat gaattcgttt caataatgaa tcagatgtat tcaggttaca
      421 attcggacag ggttcaggat atggcaaaag tcctgaaaca tattttccag aactgtaagg
      481 tctgcgtatt tgactccctc attcagtctt ttgaaattga tttcatttat gctctttccg
      541 gagaaaatcc tggggcggat attgagatac tttctaatga gtttctgact attaaaaaac
      601 gtccattatt cagaacagag gacaaaggtt tttttgaaaa gaaccttatt gaagcagtga
      661 aatccggaac aaaagtttta tttcttacag acgataaatc atactctgaa tttattacat
      721 cacagattga agactctgct gaagttgata ccgattcaag cacagtttca attctgtcac
      781 gggatacaag gtctgaactt ctggacggca gaacgattgc agattttatc agagaaaaaa
      841 aaccgaaact gctttcagta actccgacag gttttgtcgg tttggatatt ccggaaggat
      901 cttttgatat ggtgtatatg tacggaggta atttcggaat cagggattac aggatttaca
      961 ttcaggcact gcacagactc agagattatt ctgcacccgc tgtattctat tcaaacaagg
     1021 gcacttatga cccgatgctt cttacaagtg cagactcaat cctggtcaaa agagctgaat
     1081 cgaataacaa gctgaatgat aagatttttt atgatttagg aaaagatgga ttctggaaac
     1141 ctaaagagga atattctgcg tttgaaaatc tctctgctga actggaggca taccagaata
     1201 gaaccgctaa aattggtcta gctcagtata tctctgattt cttcaatgag agattcggat
     1261 tcgaaacgtc cattctgaga gagaaatctg aatccctgga aagaccatct ctcaaagaac
     1321 ggactgagga aaagaagaac agactgaaag cagtcaagct gatagacaag caggaatatg
     1381 attctctgag aaggaatcag gcaggcaggg acttgaacaa ggaggaaact gcacttgtcc
     1441 agaaatatga aattgctgaa aagttttcaa ttggacatac ggacattcag agaaatgaaa
     1501 aggtaaattt aattatagat tataccgcaa accctgataa aatgagggtc attgcagaaa
     1561 atttcgaatg gctgaagaac tccgattcag tgaactatga gaaagaaaaa gcagattatg
     1621 aaaagacttc cggaataggg cagactgcaa aatcaaagac cctcgaaact atgcttctga
     1681 agaaaattct gtcttccctg gacggaaagg aattcgggaa aactgacatt ccgatgagtc
     1741 tgatttatga actggagaca gtgaccggca gacagttcac tgcctcttat aatgatgcaa
     1801 ttctggacag aaaatgcttc gcagatctga agggcagact tcagtatgat gctcttgccc
     1861 ttgactatgc aaagagcatt tctgaaggag atgagaatat tatcagagaa gtcttcaaaa
     1921 gtgatattat gaaaatcttc gattctgaaa tgggaaaact ggacaagcgg aaaaagcgga
     1981 ttcagtcacg ttctcagaat cctgggctga ttcagaagca tgaagaagaa gcatcaaaaa
     2041 aggctgcgga ggcaatcaaa aagagagttt caaagttcat tcaatctaaa cggaatgact
     2101 ttgcagtttc tcctttgagc attctgtctg actggctttc aaac
//
LOCUS       DAOQGJ010000591         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34503_length_2144_cov_3.169459, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000591 DAOQGJ010000000
VERSION     DAOQGJ010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34503_length_2144_cov_3.169459"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PK683_09920"
     CDS             complement(<1..358)
                     /locus_tag="PK683_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08807.1"
                     /translation="MNPELEPYLQATIESSNRSRYITFVITFISMIIFGGYWNSLEFS
                     WFKTANDVNGCLRLYISFAENINKDLTEPKKTAQNGKIEIQKADTHPAIFRELVKSKF
                     KEIYPKRFGKCEKYIHT"
     gene            complement(355..1341)
                     /locus_tag="PK683_09925"
     CDS             complement(355..1341)
                     /locus_tag="PK683_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08808.1"
                     /translation="MTNLLISIHGINPVSEPKDLTKQFRDFFGRLSLSNRNLAALFPE
                     ENRLYIQWGQPLPDSPVTDLNTLGYAQQKIYELCNYDSVRTNPSPNSILLHQILSTDP
                     VMLSKLAILMRENLVLFGFGDAVYYSSDEGEKIVRGFIYEQILNLLEKHKNDSEIRLH
                     FIGHSLGVTVCHDLMYGLASQENDYSPDYLNYPDISSEMKKSFSFWRNELIQNGRLKI
                     GSFTSMASQLPLFIMRKHKVMYNKILTNKKLSIQDLGILADEEKIKWQLFYDIDDVLG
                     FCTRPLYDDSKNNIKEIQVDCGDDPLTAHIGYFTNPTVIRETAKLLERNGKA"
     gene            1934..>2144
                     /locus_tag="PK683_09930"
     CDS             1934..>2144
                     /locus_tag="PK683_09930"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNH08809.1"
                     /translation="MRTLSLDIPEELLISFKVPRQYWKSELKKELAIQLYREGLISFG
                     NARRLAEMSKWDFHDLLGNRKIPRQY"
BASE COUNT          664 a          404 c          387 g          689 t
ORIGIN      
        1 cagtgtgtat atatttttca cattttccga atcgttttgg gtatatttct ttgaattttg
       61 atttaactaa ttctcgaaat atagcagggt gagtatctgc cttctgaatc tcaatttttc
      121 cattttgagc agttttcttt ggttccgtca gatctttatt aatattttca gcgaaactta
      181 tataaaggcg aagacagccg tttacgtcat ttgcagtttt aaaccagctg aactccaatg
      241 aattccaata tcctccaaaa ataatcatac tgataaaagt gattacaaaa gtaatatatc
      301 gtgaacggtt ggaggattct atagttgcct gcagatatgg ctccagttcg ggattcatgc
      361 cttaccgttc ctttccagca attttgctgt ttccctgatt accgtaggat tggtaaaata
      421 accgatatgt gctgtcaaag ggtcgtcacc gcaatccact tgaatttctt tgatattatt
      481 ctttgaatca tcatagagag gtcgtgtaca gaagccgaga acatcatcta tatcatagaa
      541 aagctgccat ttgatttttt cttcatctgc caaaatacca agatcctgaa tggaaagctt
      601 tttatttgtt aatattttat tatacattac tttatgttta cgcataataa agagcggcag
      661 ttgagatgcc attgatgtaa aagagcctat tttcagtctt ccgttttgaa ttaattcatt
      721 tctccaaaag gaaaaagatt tcttcatttc agaagaaata tcgggataat ttagataatc
      781 tggagaataa tcattttcct ggcttgccaa gccatacatc aaatcatgac acactgttac
      841 tccaaggctg tgcccaataa aatgaagtct tatttcagaa tcgtttttat gtttttccag
      901 caagtttaga atttgctcat aaataaaacc ccttacgatt ttctcacctt catcagatga
      961 ataataaaca gcatcaccaa atccaaacag aacgagattc tctcgcatta aaattgccaa
     1021 tttcgataac attacaggat cggtgctaag aatttgatgg agaagaatgc tgttcggaga
     1081 tggattggtt ctgacggaat cataattgca cagttcataa attttttgct gagcatatcc
     1141 cagagtattt aaatctgtta ctggagaatc tggcaaaggc tgtccccact gaatgtacaa
     1201 cctattttct tctggaaata aagcagcaag attcctattt gacagtgaca gtctgccgaa
     1261 gaagtctcga aattgctttg ttaaatcctt cggttccgac actggattaa ttccgtgaat
     1321 tgagattaac agatttgtca ctgacatctc tttctcctag caagaataaa agggaaaatc
     1381 atcccaagct ccggaaaatt cggaagttga aaataataaa acagccggct tcggaatcga
     1441 aaccagaaaa aagataaagg attggaaaat ttaggattat ttgagggata tggaagattg
     1501 aataatctga ttcgctgatt cgctgtccgc tgtccgctgt caaaatagag acttccacaa
     1561 gaaatccatt tgcgaaaaca aatagattat cgcaagaaaa aaaagtattt cttagaataa
     1621 attttttata aaatgaccaa tgatctaaac agcagtccat cggtcaatac gcagaacagc
     1681 gtacttttta tggctttaac tcaccttacg cacttcgtgc ttccggtgag tcctgccgca
     1741 agcttggacg cctcgaattt aatttttggt cctattccct attccccgtg gttaacattt
     1801 tttttcacca cggggacaag gggattgtct ttcctcattc gctattccct ctgattttgc
     1861 tctgcctgtc gttctttaaa taacttttac atttctgatt gacaattctg caaaatttga
     1921 aaagacttaa cttatgagaa ctttatcctt ggatattccg gaagaactgc tgatcagttt
     1981 taaagttcct aggcaatact ggaaaagcga attaaagaaa gaacttgcga tacagcttta
     2041 cagggaagga ttgatttcct ttggaaacgc acggcgactg gctgaaatga gcaaatggga
     2101 ttttcatgac ttgctaggaa atcgaaagat tccaagacaa tacg
//
LOCUS       DAOQGJ010000592         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34588_length_2141_cov_2.726750, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000592 DAOQGJ010000000
VERSION     DAOQGJ010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34588_length_2141_cov_2.726750"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PK683_09935"
     CDS             complement(<1..998)
                     /locus_tag="PK683_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNH08810.1"
                     /translation="MSENTGEKKLNLKEYLLFFGAGLFLFFLAAFAVVLVRTRGGSKV
                     MMPDLVGQNYTDIHNELMRLRLKVRLETRNYGDKNDGEILYQSITPGKTIASGNKLYL
                     TINVGVNRIIVPDLKGQQLTSAKSLLEKVLSGETYVTLPLGGVTYIPAENGQSPGTVV
                     DQIPEAGKITTNREKVYLLVTEEKTLNGQSGLDKFEGQPLQFAAMSLSKRKMQFKVNE
                     IISTKDKKENGLVAKSEKNGENYQFSVNYFAPDISYHSGFEKIEYKVGKSGKFNGRIV
                     SEDIEKDEGKEVFRELNLSSGEVWKFVPFRVGNSRLELIDSATGSKEKSFAFNAELR"
     gene            complement(995..1924)
                     /gene="fmt"
                     /locus_tag="PK683_09940"
     CDS             complement(995..1924)
                     /gene="fmt"
                     /locus_tag="PK683_09940"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNH08811.1"
                     /translation="MKIGFFGTPEHSAELLRTLVREKYEISFAAANPDKPQGRDRKLE
                     FPPVKKTALELGIPVIQHISVKTEEAIQNILSYDADIYIVFAFGSIIPRRIFDHPRGG
                     TVNLHGSLLPEFRGASPVQAAILAGYRETGVTLQYITEELDAGDIISSAKVPVADDDN
                     FQTLLDKISRSGTELILTLLKNFKGGKASAIPQDHSRATFCRKIRPEDRKLDFSKPAE
                     ELHNQIRAFNPGNICFTSFRDKRVNVYRTRVSEKKEGKTGSFTAVDRKTPGVICGDGR
                     VLILEELQLENKKVMKGLDFMNGSRPTEGEDFK"
     gene            complement(1924..>2141)
                     /locus_tag="PK683_09945"
     CDS             complement(1924..>2141)
                     /locus_tag="PK683_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000641110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNH08812.1"
                     /translation="KAIGREASENTFMLGPAECPFYRVDSNFRRHIILKTHDIQKIRE
                     ILKKTVLPINLPSGLYLEIDIDPVDLV"
BASE COUNT          522 a          499 c          426 g          694 t
ORIGIN      
        1 cggagctccg cattaaaagc aaatgatttt tcttttgatc ctgttgcaga atcaatcagt
       61 tcaagtctcg aattgcctac ccgaaaaggt acaaatttcc agacctcgcc tgagctgaga
      121 ttcagttcac ggaagacttc ttttccctcg tccttttcaa tgtcttcgga tacaattctt
      181 ccgttgaatt tccctgattt gccgactttg tattcaatct tttcaaatcc agagtgatag
      241 gagatgtccg gtgcgaaata atttacagaa aactgatagt tttctccgtt tttttccgac
      301 ttggcgacta gaccgttttc ctttttgtct tttgtggaaa tgatttcatt caccttgaac
      361 tgcatttttc tcttggaaag actcattgct gcaaactgaa gaggctgacc ttcaaatttg
      421 tccagtccag actgaccgtt cagagttttt tcttcagtga caagaagata cactttttcc
      481 ctgttggttg tgatctttcc tgcttcggga atctgatcca ctacagtgcc gggtgactgt
      541 ccgttttccg caggaatata ggttacacct cccaaaggca gggttacata tgtctcccct
      601 gagagaactt tttcgaggag tgattttgca cttgtcagct gctgaccttt cagatcaggc
      661 acaataattc tgttcacgcc tacattgatg gtcagatata atttatttcc agatgcaatt
      721 gtcttgccgg gagtgatgga ctgataaaga atctctccgt cgttcttatc tccgtaattt
      781 ctcgtttcca gacggacttt cagtctgagt ctcatcagtt cattatggat atccgtataa
      841 ttctgaccga ccaaatccgg catcattacc ttggaacctc ctcgggttct caccaaaacc
      901 actgcaaaag cagcaagaaa aaacagaaac agtcctgctc cgaaaaacag aagatactct
      961 ttcaggttca gtttcttttc tccagtattc tcgctcattt aaaatcctca ccttcagtag
     1021 gtctcgaccc attcataaaa tcaagacctt tcataacctt tttattttcc aattgaagtt
     1081 cttcaagaat taaaactctt ccgtccccgc agatgactcc gggggtcttt ctgtcgactg
     1141 cagtaaagct gccagtcttt ccttctttct tttcagaaac tctggttctg taaacattga
     1201 ctcttttgtc tctgaatgaa gtaaagcaga tatttcccgg attgaatgcc ctgatctgat
     1261 tgtggagttc ctccgcaggc ttggaaaagt ccagttttct gtcttccggt ctgatttttc
     1321 tgcagaatgt tgccctgctg tgatcctgcg gaatggcact ggctttccct cctttaaagt
     1381 ttttcagaag cgtcagaata agttctgtac ctgaacggct gatcttatca agaagggtct
     1441 gaaaattgtc atcgtctgca acaggcactt tcgcagagga gataatatct cctgcatcca
     1501 gttcttccgt aatgtactgg agtgtgactc ctgtttctct gtatcctgca agtattgctg
     1561 cctgcacagg agatgctcct ctgaattcag ggagaaggct tccgtgcaga ttgactgttc
     1621 cgcctctcgg atgatcaaat attcttctgg ggattatcga tccgaatgca aatacaatgt
     1681 atatatccgc atcgtaggaa agaatattct gtattgcctc ttccgtcttg acagaaatat
     1741 gctgaatcac agggattcca agttcgagag ctgttttttt tacaggagga aattcaagtt
     1801 tcctgtctct tccctggggt ttgtcagggt ttgccgctgc aaaactgatt tcatatttct
     1861 cccgcacaag cgtgcgcaga agttctgcag aatgttctgg ggttccgaaa aatcctattt
     1921 tcatcagact aaatccaccg ggtcaatgtc tatctcaaga taaagtccgg aaggcaggtt
     1981 gattggcaag acagtttttt tcagaatttc acggattttc tgaatatcat gagtcttcag
     2041 tatgatatgc cttctgaaat tggagtccac cctgtaaaaa ggacattctg ccggcccgag
     2101 cataaatgta ttttcagacg cctccctgcc tatggccttt c
//
LOCUS       DAOQGJ010000593         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34613_length_2140_cov_3.186571, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000593 DAOQGJ010000000
VERSION     DAOQGJ010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34613_length_2140_cov_3.186571"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PK683_09950"
     CDS             complement(<1..456)
                     /locus_tag="PK683_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNH08813.1"
                     /translation="MKTSFLVLILSVFTAKTLSAQNFQDPKLKTAPIPGKVNTDYQEF
                     GPSLTKDGKTLYFYSKRGGAKYSNLYKSKLIDGKWSEPAELRGLNSEYDDQSPYIDLD
                     EKFIIFSSNRDGSVEFTLPSGQKGVSRDLYYAEFHNGRWSRPFSISEIIN"
     gene            670..>2140
                     /locus_tag="PK683_09955"
     CDS             670..>2140
                     /locus_tag="PK683_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH08814.1"
                     /translation="MGPFSNEQFTLHDLIVLNRKDEFLKRISSDNLDSVNSDGLTLLH
                     TALKERNLEISTILIEKGADVNAKGFNGFTPLHYAILINVDLWEKDDDELIDIFPLLE
                     LLLKRGANVNELTEGGSTPLHFLIECYDYELDILKACKLFIENGAAVNVMDRHFKTPI
                     FYAVDRESTELVKLLIENGADVNLGKYDSFPIIEANYKKESPDIVKLLLDNGADASVC
                     EDEDSYETALSEQMFAGGNEQIVKMLLEKGADVNSLGLGEESPLHYAACYNLISTMKL
                     IFEYKPTVNLKNYNGNTALHYAGTKGNFEAAVLLIQNGADINIKNNENQTAFHFAAVN
                     NHISLVQYFLETGYDLGLEERNFILCDMIMQEEMNFEIADLLLGEDMDIKDLYREGKP
                     LLYFALEEIDRRKVFYLLEKGADPESVPDWNSLSIYAVSGHTQKVKDLLKTGRAVFSL
                     GVDDRDPINLAIQNQNRHIAKTVYKEAVKIGLPMKWDYFD"
BASE COUNT          673 a          352 c          458 g          657 t
ORIGIN      
        1 gttgatgatt tcagaaatag agaaaggtct gctccatctc ccattgtgga attccgcata
       61 atataaatct ctggatacac ctttttgacc tgaaggcagg gtgaactcga ctgagccgtc
      121 gcgattggat gaaaatataa tgaatttttc atctaaatct atatacggac tctgatcatc
      181 atattccgag ttcagccctc tgagctccgc aggctccgac catttcccgt ctatgagttt
      241 cgacttgtaa aggttggaat acttggctcc tcctcttttg gagtaaaaat acagggtctt
      301 cccgtctttt gtgagagagg gtccgaattc ctggtaatcc gtattcacct ttcccggaat
      361 gggtgcagtc ttcagtttag gatcttgaaa attctgtgca gacagagttt ttgcagtaaa
      421 tacagacaat atgagtacaa gaaatgaagt cttcatggta aacctatcgg aaaaaggttt
      481 ttgaagttaa cagccttcag aagagaaatt ggaacagttt tcctccaatt tccgttattt
      541 ttcccttttc tagtttttga aaaaaggaaa aacctgacag agattttttt ccggtaaagc
      601 ggcaatcaga tcagcctgag cttctctttt ttatttgcat tccatcggct ctgcgctgag
      661 atatatgata tgggaccatt ctcaaatgag cagtttactt tgcatgattt aattgtactg
      721 aatagaaagg acgaatttct gaagaggatt tcctcagata atctggattc cgtaaattca
      781 gatggactga cacttcttca tactgcactg aaggaaagga atctggaaat ttcaactatc
      841 ctgatcgaaa aaggcgcgga cgtaaatgca aaaggattta atggttttac ccctcttcac
      901 tatgcaattt taataaatgt agatctttgg gaaaaagatg atgatgaact aattgatata
      961 tttccgcttt tggaattatt gttaaagaga ggagcaaatg taaatgaatt gactgaaggc
     1021 ggatcaaccc ctcttcattt tcttattgag tgttatgatt atgaattgga tattttaaaa
     1081 gcctgtaaac tgtttattga aaacggtgct gcagttaatg ttatggaccg tcattttaaa
     1141 acgccgattt tttatgctgt cgacagggaa agcacagaac tggtgaaact tcttatagag
     1201 aatggagctg atgttaattt agggaaatat gattcatttc ctattataga ggcaaattat
     1261 aaaaaggaat ctcctgacat tgtgaaacta ctgctggata atggtgctga tgcaagtgtg
     1321 tgcgaagatg aggattctta tgagacagcc ttgagtgagc agatgtttgc gggcggaaat
     1381 gagcagatag tgaagatgct tttggaaaaa ggagcagatg tcaacagcct cggtcttggt
     1441 gaagagtctc cgcttcatta tgccgcatgt tataatctga tatccacaat gaagctgatt
     1501 tttgagtata aaccgacggt caatctaaaa aattataatg gaaatactgc acttcattat
     1561 gccggcacca aaggcaattt cgaagcagcc gtattactga tccaaaatgg cgcggatata
     1621 aatattaaaa ataatgaaaa tcagacagca tttcattttg cggcagtaaa taatcatatc
     1681 agcctagttc aatactttct ggaaacagga tatgatttgg gtttagaaga aagaaatttt
     1741 attttatgtg atatgataat gcaggaggaa atgaactttg aaattgcaga ccttctttta
     1801 ggagaagata tggatataaa ggatctgtat agggaaggta aaccgcttct ttattttgca
     1861 ttggaagaaa tagacaggag aaaagttttt tatctacttg aaaagggtgc agatcccgaa
     1921 tctgttcctg attggaattc tctgtcaatc tatgcagttt caggacatac tcaaaaagta
     1981 aaagatcttt taaaaacagg aagggcagtt ttttctcttg gagttgatga tcgtgatccg
     2041 atcaaccttg caattcagaa tcaaaacagg catattgcaa agacagttta caaggaagca
     2101 gtaaaaatag gcctgccgat gaaatgggat tattttgaca
//
LOCUS       DAOQGJ010000594         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34614_length_2140_cov_3.178897, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000594 DAOQGJ010000000
VERSION     DAOQGJ010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34614_length_2140_cov_3.178897"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PK683_09960"
     CDS             <1..376
                     /locus_tag="PK683_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidyl aminopeptidase"
                     /protein_id="HNH08815.1"
                     /translation="DSPYMDLDSIIRERAEKDYGKILLPLVPITMWTAGLRAGFNPEA
                     VNIKKAAKKIRIPVFISHSRQDDFTPPNHSEQIFENVETEKKVLHITDWGASHARSIN
                     EKPEEYEKQMDDFIERYVPSFL"
     gene            441..1373
                     /locus_tag="PK683_09965"
     CDS             441..1373
                     /locus_tag="PK683_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH08816.1"
                     /translation="MKTRILKWFLFLLTVSLFLFFGIVWYFSGVIVAFKTKSHNEIKE
                     RNKVSGPSQFGLPENPEDIEIPAGELKLSGWFFKNPRKNKKCGVVIAHGHANNRYGVL
                     KYSPAFWKRGCDLLFFDHRHHGISPGEFGTYGYHERKDVLSAVEVLRQKTGLEESRIG
                     IFGESYGASTSLLAACLNDRLAFVGADSPYMDLETIVTEKAEAQYGKMLLPILPPTFW
                     VAGKRADFDPYEVNVKEAVKNLHIPVFISHSAQDKYTVSRHSELIYQNISYDKKMLHI
                     TNWGAEHARSMDVNPKEYEKHLDEFIEKYVPAFK"
     gene            1460..>2140
                     /locus_tag="PK683_09970"
     CDS             1460..>2140
                     /locus_tag="PK683_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNH08817.1"
                     /translation="MEKDELEILKSGFHLYEASAGTGKTYKVVDIIVNFIIEGISLKE
                     LAVLTFTEKAGGELQERVRRKLGEVLRGTADDVKIELTEERRKHLTEQLEVLSTAKIG
                     TIHQFCRTLLRKYTIEAGLSPSWQEIRETEIYEKVFMDYFGTVEKFEEEDLKSFLKDC
                     GWDVLKNFCIQSFSRTGFVEFDEIKKSDYNEEDYIRVYKNFLNAVQDIPAKHLTKLKA
                     EEASQASAD"
BASE COUNT          673 a          396 c          500 g          571 t
ORIGIN      
        1 agattctcct tatatggact tggacagcat tattcgggag cgggcagaaa aggattacgg
       61 aaaaatactt cttccccttg ttcctattac gatgtggact gccgggcttc gtgcaggatt
      121 caacccggaa gcagtgaata taaaaaaagc tgctaaaaag atcaggatac ctgtattcat
      181 ttcccattcc aggcaggatg attttactcc tccaaatcat tcagagcaga tttttgaaaa
      241 tgtggaaaca gaaaagaagg tacttcatat tactgactgg ggcgcttctc atgccaggtc
      301 aatcaatgaa aaaccggaag aatatgaaaa gcagatggat gattttattg aaagatatgt
      361 gccgtccttt ttatgatctc aaaattttct tttcagaatg cagaacagaa tgaattctgc
      421 tatttaagca ggctgtcatt atgaaaacac gcattctaaa atggttccta tttttactta
      481 cagtatcctt attcctgttc ttcgggattg tatggtattt ttccggggtt atcgttgcat
      541 tcaaaacgaa atctcacaat gaaatcaagg aaagaaacaa ggtgtcaggg ccttctcagt
      601 tcggtctgcc ggaaaatccg gaggatatcg aaattcctgc aggggagctg aaactttccg
      661 gctggttttt caagaatccc agaaagaaca aaaaatgcgg cgttgtgatt gcacatggac
      721 atgcaaacaa caggtacggc gttctgaaat attctcccgc attctggaaa agaggatgtg
      781 atctgctttt ctttgaccat cggcatcatg ggatcagtcc tggagaattc ggaacatacg
      841 gatatcatga aagaaaggat gtgctgagtg cagtggaagt gctgagacag aagacaggcc
      901 ttgaggaaag cagaatcggt attttcggag agtcctacgg tgcgagcaca agtcttttgg
      961 cggcatgtct gaatgacagg cttgcttttg tcggcgctga ttcaccttat atggatctgg
     1021 aaacgattgt aactgaaaaa gcagaggcgc agtacgggaa aatgctcctt cccattcttc
     1081 cgccgacctt ctgggttgca ggcaaacgtg cagacttcga tccttatgag gtcaatgtaa
     1141 aggaagctgt aaaaaatctg catattccag tgttcatttc tcattccgca caggacaaat
     1201 acactgtttc gaggcattca gaactgattt atcagaatat ttcctatgat aaaaaaatgc
     1261 ttcatatcac aaattggggt gcagaacatg cccgctcgat ggacgtgaat ccgaaggaat
     1321 acgaaaagca tcttgatgag ttcattgaga aatatgttcc tgcattcaaa tagagaacgg
     1381 aatattcaac ggcttactga aatatatgtt cattcacatc tgtcctattc cgtcgccgta
     1441 aatcccggct gaagacagta tggaaaagga cgaattagaa attctgaaat ccggctttca
     1501 tctttatgag gcatctgcag gaacaggaaa aacatataaa gtagtcgata ttattgtaaa
     1561 tttcattatt gaaggaattt cactgaagga actggcggtt ctgactttca ctgaaaaggc
     1621 cggaggcgaa ctgcaggaaa gggtccgcag aaaacttggt gaggttctga gaggaactgc
     1681 agatgatgta aaaattgaac ttacggaaga aaggcgaaaa caccttactg aacagctcga
     1741 ggttctgagc acagccaaga tcggtacaat tcatcagttc tgcagaacac tgctcaggaa
     1801 atatacaata gaggccggtc tgtctccgtc ctggcaggaa atccgagaaa cagagatcta
     1861 tgaaaaagta tttatggatt atttcggaac agttgagaaa tttgaagaag aggatctgaa
     1921 aagtttcctg aaagactgcg gatgggatgt gctgaaaaat ttctgcattc agtcattcag
     1981 caggacaggt tttgttgaat ttgatgaaat taaaaagagt gactataatg aagaggacta
     2041 tatacgagta tataaaaatt ttctgaatgc tgttcaggac attcctgcaa agcatctgac
     2101 aaagctgaag gcagaagagg cttctcaggc atcggctgat
//
LOCUS       DAOQGJ010000595         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34638_length_2139_cov_3.439060, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000595 DAOQGJ010000000
VERSION     DAOQGJ010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34638_length_2139_cov_3.439060"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PK683_09975"
     CDS             complement(<1..202)
                     /locus_tag="PK683_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08818.1"
                     /translation="MFSPETSVIFHLIFETAGFLIGFHYYLHLRKKERDLLTEKERYW
                     ILISVITGALIFSRFIAVMGHLD"
     gene            1133..>2139
                     /locus_tag="PK683_09980"
     CDS             1133..>2139
                     /locus_tag="PK683_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH08819.1"
                     /translation="MAVLLQDTAFYNKSSDKASPSSAAPVNEFVQPPATFGGSIPQAF
                     LSVSEFGVSRYDDLIVIFSHSMNTSTIVSAGSGTDSFILKDSLGNQVLGRFFWFGTKK
                     LYFDPYKELKSYEYYTLTLTSDAKTTANVPLTFYSKTFRTRPGYNPVLTLTGLNGGVP
                     MANQSVCVDDSNPTPGAPVPPLPSTCYNHERGFLFDSAQTPQMELNVSVNDSSTVSSM
                     KLCKLSVGGVNTASAPKRCREDKIKPDEDLSDPGIELCSVCSASTSVRLDTLTDSFLK
                     LSKGTNNYYLEIIDIYGNVTQKNINFQWAPHLSDSLTPPNLTASTSVIIDKSNSVSSA
                     N"
BASE COUNT          651 a          444 c          423 g          621 t
ORIGIN      
        1 gatccaaatg ccccattacg gcaatgaacc tggaaaaaat cagtgcaccg gtgatgaccg
       61 aaatcagtat ccagtatctt tccttctccg tcagaaggtc cctttccttt tttcgaagat
      121 ggagatagta atgaaatcca atcaggaaac ctgctgtttc aaaaatcaga tggaagatga
      181 ctgaagtttc tggagagaac atattcacaa tgatattctg gctcggtaag aaaaaaaacg
      241 gcaagcataa aatcagactt ggggtcagct gaattctttc tggctgcatc cgaaacctaa
      301 gcacaggata gcgttgaggc tgctggaatc tccaattact gcctgaaatg cacctttgtc
      361 attatactca ttttcataaa taattgcgcc ttttccggct gacagacttg attttactga
      421 gtcgtgaaca aataaatacg caaagacttt tgaaaacgag tataaccatt cctcctgaaa
      481 tccgaatttt agagtttact gaacaatgct gacaggttaa ccccttggtt ataacgggag
      541 actgagtata tgaggaaaga tgcccctcgc agctgaaacc ggaaagagca ctcgaggata
      601 agtgcccaat tttggatatt tctgagtaaa gcttgctttc tttttaaatg acagattttc
      661 gcagatttag ttctacaatc tacttatatc gatcatccgt tataaaaaaa gagttgcaaa
      721 tttaacccca taattatagt ggtaaagaac tttttgaaaa atgtgaatag aaaaaaagta
      781 acctgaactt tagctgcttc ggttacaaat ctgagctgga aagaatagtc ggaacttagg
      841 tccctgtttc aggcaggaat aaataaattc ttgctttctc aaaatataat gatttgatga
      901 gaatttatat tctctgcatt gtcattaaac agactgattt gactttacag tctggattac
      961 caaagacctg agctgcactg ccgcatttaa ctgagtactt ggggatagct gcaccaagta
     1021 tgttttaatc agtttcaatt attagaccga ggggtatatg aaaactgcac tttgtttgct
     1081 ctgtatgctg attttaaact gcagcataaa aaagggaaag ctggaatttc cgttggcagt
     1141 tcttctgcag gatactgctt tctataataa aagttcggat aaagcctctc cttcttcagc
     1201 tgcgcctgtc aatgaatttg tacagcctcc tgccacattc gggggaagca ttcctcaggc
     1261 ctttctgagt gtttcagaat ttggggtcag ccgatacgat gatttgatcg ttatattttc
     1321 acattcaatg aatacttcca ccatcgtttc agccggatca gggactgatt cttttatttt
     1381 gaaagattct cttggaaacc aggtcttggg aaggttcttc tggtttggta caaagaaact
     1441 gtactttgat ccttacaagg agctgaaatc atacgaatac tacacattaa cactgacatc
     1501 tgatgcaaaa accactgcca atgttccttt gaccttttat tcaaagacat tccggactag
     1561 accaggctac aatccagtcc tgactttgac cggtttgaat ggaggtgtac cgatggcaaa
     1621 tcagtcagtt tgtgttgacg attccaatcc tacaccggga gcacctgtgc caccgcttcc
     1681 cagcacctgt tacaaccatg aaagaggctt cctttttgac tcagcacaga cccctcagat
     1741 ggaattgaat gtttcagtaa atgacagctc aacagtatct tcaatgaaac tctgtaaact
     1801 ttctgtagga ggagtaaaca cagcatctgc tccaaaaagg tgcagggaag acaagataaa
     1861 gccggatgaa gatctgagtg atcctggaat tgaactctgt tcagtctgca gtgcaagcac
     1921 atctgtcaga ctggatactt tgactgacag ctttttgaaa ctaagcaaag gtacaaacaa
     1981 ctactatctc gaaattattg atatttacgg caatgtcact cagaaaaata tcaacttcca
     2041 gtgggctcct catctttctg acagcctgac tcctccaaat ctgactgcat ccacaagcgt
     2101 aattatcgat aagtcaaatt ctgtttcatc ggcaaacag
//
LOCUS       DAOQGJ010000596         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34671_length_2138_cov_3.375900, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000596 DAOQGJ010000000
VERSION     DAOQGJ010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34671_length_2138_cov_3.375900"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PK683_09985"
     CDS             complement(<1..688)
                     /locus_tag="PK683_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNH08820.1"
                     /translation="MNIHFLSKKTKIVCTIGPASNNEETISKLIDAGMDIARMNFSHS
                     THSEHKKVFDMLRMCELRAGRPLGILADLQGPKIRTGKIEGNEFETKEGDLIWLNTKT
                     DFLGNREEISTTYTHFVDDVNVGDRVLIDDGKIVLIVRKKEKERVQLETIVPGKIKNN
                     KGINLPGTNITAPALSEKDILDLKFALELGVDFIALSFVRRAKDLELAKSYMGDTFTG
                     LIAKIERPEAI"
     gene            complement(756..>2138)
                     /locus_tag="PK683_09990"
     CDS             complement(756..>2138)
                     /locus_tag="PK683_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH08821.1"
                     /translation="LMFSILRAAVARVFKAESQLDKHLQKFSGFRTDLIAKLNQNKVR
                     INNLKVIDYLQSLHSETYKKAEKVYFETQASNGFMYFIVRGEVKSVKRVGDKNLVLHT
                     LEAGDFFGDVSFFTSRARLSTMLVTSDHAEIVPIDREVFMKIVEIYPEVVLLELKSFI
                     WKLINTEKANNALKTELENEKKEADIRSVKKGEVIIAENDPSNETMYFILNGEFSVFK
                     NRGENKKELVNILRKGDFFGELSLISNQPRTATIAVRSDSADLILFSKRKFIEQTKQN
                     PSLMMSILKATIARLLRAETTLDKLIKKIPDIDSNLEISLKESRIENINIFKYVHSVY
                     SSNLKKGDRIFGEGDASNGMMFFLLQGSVSVVKIYNKRIYQITNLEAGDFFGEVSLVA
                     TMPRYQTMIVSSERAKIASIDIKILQKIIQINPGFLLSLLRTVIWKLIIMEKAVTKLN
                     IEYDMYEKKI"
BASE COUNT          553 a          464 c          415 g          706 t
ORIGIN      
        1 atattgcttc cggtctttca atctttgcaa tgagtcctgt aaatgtgtct cccatataac
       61 tttttgcaag ttccaggtcc ttggctcttc tgacaaaact cagtgcaatg aaatccactc
      121 ccagctccag tgcaaatttc agatccagta tatctttctc agacagtgca ggagcagtaa
      181 tatttgtccc cggaaggttg atgcccttgt tgttcttgat tttaccggga actatggttt
      241 ccagctgaac tctttctttt tctttttttc tgacaatcag aacaatcttt ccgtcatcta
      301 tcagaaccct gtcgccaaca ttcacatcgt ccacaaaatg agtatatgtg gtgctgattt
      361 cttctctgtt tccgaggaaa tctgtctttg tgttcagcca gatcagatcg ccttcctttg
      421 tctcaaattc gttcccttct atttttccgg ttctgatctt aggcccctgc aggtcagcaa
      481 ggattccaag cggtctgcct gctctcagtt cacacatcct cagcatatca aaaacctttt
      541 tatgttcaga atgagtggaa tgagaaaagt tcattctggc aatatccatc cctgcatcga
      601 tcagtttgga aatggtctct tcattgttgg aagcgggtcc gatagtacag actattttgg
      661 tttttttgga aagaaagtga atgttcataa tgactccttg cgctgcaggc aattgatgaa
      721 tttatttagt ctggaaaatc cgtcagtaaa tttattcaga tttttttctc gtacatatcg
      781 tactctatat taagcttggt tacggctttt tccataataa tcagtttcca gattactgtt
      841 ctgaggaggc ttaaaagaaa acctggattg atctgaatga ttttctgaag gatttttatg
      901 tcaatggaag caatctttgc tctttcggaa ctgacgatca ttgtctgata gcgcggcatc
      961 gttgccacaa gggaaacctc tccgaaaaaa tcacctgctt ccagattggt gatctgataa
     1021 attcttttat tgtatatctt cactacagaa acgcttccct gaagcagaaa gaacatcatc
     1081 ccgttggaag catctccctc gccgaaaatc cggtcgcctt tcttcaggtt tgaactgtag
     1141 acgctgtgca catacttgaa aatatttata ttttctattc tgctttcctt gagactgatt
     1201 tccaaattgg aatcgatatc aggaatcttt ttgatcagtt tgtccagagt agtttcagca
     1261 cggagaagac gggcaatcgt tgccttcaga atggacatca tcagactggg attctgcttg
     1321 gtctgctcaa taaatttccg cttactgaaa aggatcaggt ctgcagaatc tgagcggacg
     1381 gcaattgtag cagttctggg ctgattggaa atcagcgaaa gttcaccgaa aaaatcgccc
     1441 tttctgagta tgttcaccag ttctttttta ttttcccctc tgtttttgaa aacggaaaac
     1501 tccccgttga ggatgaagta cattgtttca ttggagggat cattttctgc aataatcact
     1561 tcgccttttt tcacagaacg gatatctgct tctttctttt cgttctccaa ttctgtctta
     1621 agtgcattgt tggccttctc ggtattgatc agcttccaga tgaaagactt gagttcaaga
     1681 agaacaacct cgggataaat ctccacaatc ttcataaaaa cttctctgtc gataggaaca
     1741 atctcagcgt ggtcggacgt gactagcatt gtagaaagtc tggctcggga cgtgaaaaag
     1801 gacacatctc cgaaaaaatc ccctgcttcc aaagtatgga gaactagatt tttatcccct
     1861 accctcttta cagatttgac ctctccacga acaataaaat acataaatcc gtttgaggcc
     1921 tgagtttcaa aatacacttt ttctgctttt ttataagttt cagagtggag tgactgaaga
     1981 taatcaatca ctttaagatt gttgattctc accttgttct gattgagttt tgcaatcaga
     2041 tctgttctga acccggaaaa tttctggaga tgcttgtcca gctgggattc tgccttgaat
     2101 actctggcta ctgctgcccg gagtatggaa aacataag
//
LOCUS       DAOQGJ010000597         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34763_length_2135_cov_2.501923, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000597 DAOQGJ010000000
VERSION     DAOQGJ010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34763_length_2135_cov_2.501923"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK683_09995"
     CDS             complement(<1..390)
                     /locus_tag="PK683_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017853627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNH08822.1"
                     /translation="MSDQTLTEQRYYTAESFPKGLSRKVVESISHIKNEPGWLTEFRL
                     EAFEVYSQKEMPKWGFLNSFNVNIDDYVHYVGSNQKKKKSWEDVDPEVLRSFEKLGIP
                     EHERKYLAGIEAMNDSETVYANVKKELT"
     gene            complement(528..1112)
                     /locus_tag="PK683_10000"
     CDS             complement(528..1112)
                     /locus_tag="PK683_10000"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNH08823.1"
                     /translation="MPQVTSIAPGFEAEAVIGKEFKKIKLSDYKGKWVVLFFYPLDFT
                     FVCPTEIVEYDAKLDEFKKIGAEVLGVSIDSTFSHLAWKNTARKDGGIGDIKYPLISD
                     ITKSIARDYGVLLDDGIALRGTFIIDPKGIIRQATVNDLPVGRNIDEAIRLVKAFQFA
                     EKHGEVCPANWNEGSKTMKADVEKSKEYFSAVNK"
     gene            1640..>2135
                     /locus_tag="PK683_10005"
     CDS             1640..>2135
                     /locus_tag="PK683_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08824.1"
                     /translation="MELSEARSIISAWEKLFDVLRQEKQEARMIIQAHENLSDLVKLE
                     KKESRNAIEALIEHQRISQDEIQQKTVTLKNILNINRDIISVPGGDVLLGKIVESILH
                     VLNAQRGLLWMKKENGMKFSSLVNISESELQDTSFQASLRVMTDAVEEKKTQIRRFSE
                     YRYGK"
BASE COUNT          629 a          406 c          431 g          669 t
ORIGIN      
        1 ggtgagttct ttttttacat tggcatagac tgtttctgaa tcattcatgg cctctatgcc
       61 ggcaagatac tttctctcat gttcgggaat tcccagtttt tcaaaactcc gaaggacctc
      121 aggatccaca tcttcccatg attttttctt tttctgattg gagcctacat aatgcacata
      181 gtcgtcaata tttacattga aggaattcag aaagccccat ttaggcattt ctttctggga
      241 atacacttca aatgcttcca gtctgaattc agtgagccag cctggttcat ttttaatatg
      301 agaaatggat tcgacaacct ttctgctgag tcccttggga aagctttctg cagtatagta
      361 tctttgttct gtgagagtct gatctgacat aggttcctcg aaaatgcata tcgcagagaa
      421 gctcaaagga actgaatttg agatctccgc gctttaattt acgttttttt acgtgaactg
      481 gaatcggcat atttcacgtt ttttacagac tgaaaaaata caaggattta cttgtttact
      541 gcgctgaagt attccttgga cttttctaca tctgctttca ttgtttttga tccttcgttc
      601 cagttcgcag gacacacttc accgtgtttt tctgcaaatt ggaaagcttt cacaaggcga
      661 atcgcttcat caatgtttct tcccacagga agatcattta cagttgcctg acggatgatt
      721 cctttcggat caatgataaa tgtacctctg agagcgattc cgtcatcgag aagaactcca
      781 tagtctcttg caatcgactt tgtgatgtcg gagatcagag gatacttgat atcgccgatc
      841 cctccgtctt ttcttgcagt atttttccat gccaggtgag agaatgtgct gtcgatagac
      901 actcccaata cttctgcacc gatctttttg aactcatcaa gttttgcatc gtactctaca
      961 atttctgtag gacatacgaa agtgaagtct agaggataaa agaaaagaac aacccatttt
     1021 cccttataat ctgaaagttt gatttttttg aattctttgc cgatcacagc ttctgcttcg
     1081 aaaccgggtg caattgatgt tacttgtggc atatacaatt ctccattgtg aaatatttta
     1141 agacaagttt tttaggatcg ctgaattttt gcgagctaat ttttataatc attctaaaaa
     1201 tttaagcggc ctcccggcct tgacttgatt cctgcagatc aagcatagaa aacctgttca
     1261 aatggacaga tgagtgagaa aactgcagca gggataagga gccttttcgg tacggtctaa
     1321 gtgaatatac ctatgttcta tgatattatg gcagacagat cattctctga cggctttgct
     1381 gtcgggatca tagggaattc ttctgtaaaa ggattctgat ctttctcatt ccggtatttt
     1441 tcagattgca ggatttatca ggaaaaaaat tgagtctaat agacagaaag acagacgaag
     1501 actgatttcg aatttatttt tcataaaaat atactctgca tttcttctga atgacttccg
     1561 caaaaaggaa gaatttcgaa agaaatttca taagatgagg taaacactga acgatccagt
     1621 tgaaactgaa aaattaaaaa tggagctttc tgaagcgcgt tccattattt ctgcatggga
     1681 aaaacttttt gatgtgctga gacaggaaaa gcaggaggcc agaatgatca ttcaggctca
     1741 tgagaatctc agcgatcttg tgaagctcga aaaaaaagaa agcaggaatg caatcgaagc
     1801 actgattgaa catcagagaa tttcacagga tgagattcag cagaagactg tcactctcaa
     1861 aaacattctg aatataaaca gagatattat atctgttccc gggggagatg ttcttctagg
     1921 taaaattgta gaatccatac tgcatgttct gaatgcgcag aggggactcc tctggatgaa
     1981 aaaagagaat ggaatgaaat tttccagcct tgtaaatatt tccgaatcag agttgcagga
     2041 tacttccttt caggcaagtc tgcgtgtaat gactgatgca gtagaggaga aaaaaactca
     2101 gatcagaaga ttttccgaat atcgttacgg taaag
//
LOCUS       DAOQGJ010000598         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34781_length_2134_cov_3.576239, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000598 DAOQGJ010000000
VERSION     DAOQGJ010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34781_length_2134_cov_3.576239"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PK683_10010"
     CDS             complement(<1..1399)
                     /locus_tag="PK683_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08825.1"
                     /translation="MSSQEISLEIGCIRIYLQNENELLIPLTPFGDDLFLNRDSDFYF
                     REVQKSIEKNVSENGMDSYQDLVSLTSFHEVQKDKITVSMSKSDSAMIYDDYIMKFDI
                     YYWTVFDRKIRGKTVFPSVQSVASSKAELLSKLQNSLKLKLSMNGISDSLSLLIPYIS
                     AERTEFVSGKIKLKLLQNEDKEKEKENLAVKILEPVSFSKGSLFGMEEEWDEISEVFS
                     LEKPESFLAVGPRGCGKTKLITEFIKSFTDKNDEAVFFRQTSAPALIQNLTEGSGWES
                     RLGELAKDMHSMNAVLYLGRWSDYFEIGRYIGNSVSVGEALKDFVNKGRMTVISECTE
                     EEFAYIEQRYPGAAAGFHILRMKKRSTEELIGITENCVKEFIKDQNISINTSDITEIF
                     RLHRRFFPYSGLPGKVIRFFDSVIQNRTDLLTRHHFIQAFCTESDIPNFIIDAEIPAK
                     EDEILNFFGERVFGQN"
     gene            complement(1506..>2134)
                     /locus_tag="PK683_10015"
     CDS             complement(1506..>2134)
                     /locus_tag="PK683_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08826.1"
                     /translation="VLNTSACKEEKTTKPVQIQTPEEIRNFIKDSEWTSIAVELRPQE
                     DRTGSGKIQPFYVSRTFKFTDKDKFTGTIRSFGDPYGKLPLVQFEFKGRVEWQGEHPV
                     AKGAQKIDYVLDEGFIVTPLHQNFADALNSVPAKGLKKFELNVPQDILKKEFPLFSIK
                     EGQIVKDYDLIYIYEGMLFMGAKHVDGTPFDKPENRPHLLQIPLVRKK"
BASE COUNT          557 a          472 c          409 g          696 t
ORIGIN      
        1 cgttctgacc gaatacccgt tccccgaaaa aattcagtat ctcatcttcc ttcgcaggaa
       61 tttctgcatc tatgatgaaa ttaggaatat cagattcagt gcagaaggcc tgaatgaaat
      121 gatgtcttgt cagaagatcc gttctgttct gaatcactga atcaaaaaag cgtattacct
      181 ttccaggaag acctgaatac gggaaaaatc ttctgtgcag acggaaaatc tcagtgatgt
      241 cggatgtatt aatgcttatg ttctgatctt ttataaattc ttttacacag ttttcagtta
      301 tgccgatcag ttcttctgtg cttctttttt tcattctgag aatatggaag cctgcagctg
      361 ctccaggata cctctgttca atataggcaa attcttcttc tgtacattct gaaatgacag
      421 tcattcttcc cttgtttacg aaatccttca gtgcctctcc gacgctgaca gaattgccta
      481 tgtaacgtcc aatttcaaaa tagtctgacc atctccccag atacagcact gcgttcatgc
      541 tgtgcatatc ttttgcaagt tcccccagtc tggattccca gcctgagcct tctgtcagat
      601 tctggatgag ggcaggggca gatgtctgtc tgaagaatac agcctcgtca ttcttgtctg
      661 taaaactttt tataaattca gtgattaatt ttgtctttcc gcagcctctc ggaccgactg
      721 ccagaaaact ttccggtttt tcaagcgaga atacttcgga aatctcgtcc cattcctcct
      781 ccattccgaa aagagaaccc tttgaaaaac ttacaggttc cagaattttt actgccaggt
      841 tctctttttc cttctctttg tcctcattct gaagaagttt cagttttatt tttcctgaaa
      901 caaattccgt cctttctgca gagatatacg gaatcagaag actcagactg tcggaaattc
      961 cattcatgga aagtttcagt ttcagactgt tctgcagttt cgaaaggagc tcggctttgg
     1021 aagaagccac tgactgtact gagggaaaaa ctgtcttgcc tctgattttt ctgtcgaata
     1081 cagtccagta ataaatgtca aatttcatta tatagtcatc ataaatcatt gcagaatctg
     1141 atttgctcat actgacagta attttatcct tctgcacttc gtgaaatgaa gtaagagaga
     1201 ccagatcctg ataggagtcc atgccgtttt cagatacatt cttctcaatg gatttctgaa
     1261 cttccctgaa ataaaaatct gagtccctgt tcaggaagag gtcgtctccg aacggagtca
     1321 aagggatcag aagttcattt tcattctgta gatagattcg gatacatccg atttccaggc
     1381 tgatttcctg actgctcata aaaacagaga taagacatac ggaatggatg aaaagaagat
     1441 tttttagtcg gcactgctgt gcaaaaacag ttttttctgc tcttacacag ccccgaagaa
     1501 cccagttatt tttttcttac gagcggaatc tgaagaagat gaggtctgtt ctccggtttg
     1561 tcgaatgggg ttccgtccac atgttttgct cccataaaaa gcatcccctc ataaatatag
     1621 atcagatcat agtcttttac gatttgccct tcctttatgc tgaacagcgg aaattctttc
     1681 tttaaaatat cctgtggaac attcagttca aattttttca gaccttttgc tggaacactg
     1741 ttcagagcat ctgcaaaatt ctgatgcaga ggagtgacga tgaatccttc atcgagaaca
     1801 taatctatct tctgagcacc ttttgcgaca ggatgttctc cctgccattc cacgcggcct
     1861 ttgaattcaa actggacaag aggaagtttt ccgtatgggt ctccgaagct tctgatagtt
     1921 cctgtaaatt tgtccttgtc agtgaatttg aaggtcctgc ttacataaaa gggctgaatt
     1981 tttccggacc ctgttctgtc ttcctgcggt cttaattcta ctgcaatgct tgtccattca
     2041 gaatccttga tgaagtttct gatttcttcc ggagtctgaa tctgtaccgg ctttgtcgtt
     2101 ttctcttcct tgcaggcaga ggtattcaaa actg
//
LOCUS       DAOQGJ010000599         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34800_length_2133_cov_4.510106, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000599 DAOQGJ010000000
VERSION     DAOQGJ010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34800_length_2133_cov_4.510106"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..996
                     /locus_tag="PK683_10020"
     CDS             394..996
                     /locus_tag="PK683_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08827.1"
                     /translation="MIDMNTLETYEKLKEMHFSEEQSKGLARLFGKYFESEPASKRDL
                     SETELRLQKEIRELDLKLTEIPASSKRDLSETELRLQKEIRELDLKLTEIPASSKRDL
                     SETELRLQKEIEGIRLETQDIRLEIKNVEMKLSKEIEGIRLEIQDVRLEIKELDIRGR
                     EADGRIIQTISDSKVEILKWVAGMLIANTGLIVALSKLLK"
     gene            complement(1319..1828)
                     /locus_tag="PK683_10025"
     CDS             complement(1319..1828)
                     /locus_tag="PK683_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08828.1"
                     /translation="MIKRILTLLIFIGCTVPSFSEKKSAKDIDAVFYQLMLEHEKSMQ
                     SFFQETAWKDPFEKVFFSEVNFSRNMHTAAFFENIKTQMESGEMPAHSGVMQISYTSI
                     SSINGVRSAVSYDYRSDGKNVVLTKGTLKDGQFLQAVYNYAPETKLLETKGFKGKKLI
                     KQNTYKYDI"
BASE COUNT          637 a          412 c          443 g          641 t
ORIGIN      
        1 ctttgcttgc caatttctgg aaaacagact ttataaacga attgtccatg atgatttgga
       61 gatctctctc atcctttatt acaatttgac gaagcaatcg aaaaaatcaa tcctttctgc
      121 agtttttctg attataagtt cgacagattt acggtttttc cttttaaaat aagtaactgc
      181 tgagcggaag ataatttata gaaaagtgtt tgaaaaacgg gggtcttagg gctgcaaaac
      241 ccctcgcaag ggttggaagc ggttatgctg agcagttacg aaaatagtat ccccaaaaca
      301 tcgctgcact gcactgtttg agttttattc gcccaaagat gcaattcagc tgggaaaaaa
      361 tcagaagttg acaattcttt tacaggtatt agtatgattg atatgaatac gctcgaaact
      421 tatgagaagt tgaaagagat gcatttttca gaagaacagt ccaagggact cgccagactg
      481 ttcggaaaat attttgagtc agagcctgcc agcaaaagag acctgagcga gactgaactt
      541 cggcttcaga aggagattcg agaactggac ctaaaactca ctgaaatccc cgcctcttct
      601 aaacgggatc taagcgagac cgagctccgg cttcaaaagg agattcgaga actggaccta
      661 aaactcactg aaatccccgc ctcttctaaa cgggatctga gtgagactga gctccggctt
      721 cagaaggaaa ttgaaggaat ccgtctggaa acacaggata tccgcttgga aatcaaaaac
      781 gtagaaatga aactttctaa agaaattgaa ggaattcgtc tggagattca ggatgtccgt
      841 ctcgaaatta aagagctgga tattcgtgga agagaagcgg acggccggat tattcagaca
      901 atttctgatt ccaaagtgga gatcctcaaa tgggtagcag gtatgctcat tgcgaataca
      961 gggctgattg ttgcattgag taaactgctg aaataagttg agctgagcca gagcggacga
     1021 attgccagta cccagaaagc gagagtcata tagagtttct caactatact gatctgtcta
     1081 tttttggaag aattctaaat ttttattgac tgtacaagag atataattta gtctaataga
     1141 caggagattc ttatgaaaaa aatcattctt gctgttttgt ttgtttccct gtttgcatca
     1201 attgctcttt ctgcatgccc aggcaaaggt aagcttgtag aagatcagac tgagacaaca
     1261 ggattctgat gtttctctcc cagtctgttt ctgacagact gggattctgc tttatatatt
     1321 aaatatcata cttataagta ttctgcttaa tcagtttttt tcccttaaat cctttggttt
     1381 ctaagagttt tgtttcaggt gcataattgt acaccgcctg cagaaactgt ccatccttca
     1441 gtgtgccttt tgtcagaaca acattctttc cgtcggatct gtagtcataa ctgactgctg
     1501 aacgcacccc attgatggaa gaaatgctgg tgtaggaaat ctgcataaca ccggaatgag
     1561 caggcatttc accggattcc atttgagttt ttatattttc aaaaaaagcc gccgtatgca
     1621 tatttctgga aaagtttacc tcggaaaaaa ataccttttc aaaaggatcc ttccaggcgg
     1681 tttcctgaaa aaaactctgc atagactttt cgtgttcaag catcagctga tagaaaacag
     1741 cgtcaatatc cttagcgctt ttcttttcgg aaaaggaagg aactgtacat cctatgaaaa
     1801 taagcagtgt tagtattctt ttaatcatat attctctcct tttgtccctt agacagcaga
     1861 ctgagaaaaa ttgaaagaat tttttctatc ccaaacagat ttttttttaa cgctaatcag
     1921 atatggcttt cagtccgatg gatgcctctt catatgcagg tgagacagta ttctgtaggg
     1981 aagcattgag cagtctttcc cctcgaacag gacggagccc tgatcgaaag gattgatttt
     2041 ttatttttcc atatcaaact cggcaactgg aatgtcccaa tgaccgagca ggatttcgaa
     2101 tcttcgtttt tcttcttttt tgaatgtctg ctg
//
LOCUS       DAOQGJ010000600         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34807_length_2133_cov_3.039461, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000600 DAOQGJ010000000
VERSION     DAOQGJ010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34807_length_2133_cov_3.039461"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="PK683_10030"
     CDS             <1..1294
                     /locus_tag="PK683_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNH08829.1"
                     /translation="LFGIVLNPIIDIYLCMYIVFAHHAYYLGFKKNKNYYYLMYLWIG
                     IGFITKGPISAVIPAISIGGDILYRRDWKRLLEMKLLPGILIAAVPPLLWSLVLHEEF
                     GSFGPYFFLWLQSFGRFYKKMYNQKLDPAYFYTSFLWGLATVSVFLGICAWREGWDFF
                     SRNWAGLRLKTLQNFSKGKSDGDYVLYFWVFVYLFLIGFSRYQLPQYVYWVLPGGIIY
                     ASKFIEEISSAEFSFRSEWFYFIPQILFLSAVIAVPIFVIDAGPVYYGIIILYLVGFF
                     FLKKENAFLSALFLPSALLFALAGAEIYPALLKYQPSREIGKKLQELEPGKDGFYSFG
                     LPFSKRSYEFYSDRIIKNIADTDKFAEVLKKEGSRLVITPQEYAMFLEGYLGERFKME
                     MVGVYPAHKVATPTKEYLLKSKRESSAKKVLLIRVSLK"
     gene            complement(1298..1792)
                     /locus_tag="PK683_10035"
     CDS             complement(1298..1792)
                     /locus_tag="PK683_10035"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HNH08830.1"
                     /translation="MLPALIFFPVRADSDWRWHKYPVIRLDFSKQMAGTAEKLEVFLK
                     EQMDYIASAHGFSLTKSETYSRFEELITVQTVFYLIFSLIGMKIKTEVKTNIGRIDAV
                     IDDNHTYIFEFKLNGTKAQALQQIKDRKYHEKYTNKGKSIYLIGVEFQNRNIGEYVYE
                     IIPR"
     gene            complement(1900..>2133)
                     /locus_tag="PK683_10040"
     CDS             complement(1900..>2133)
                     /locus_tag="PK683_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08831.1"
                     /translation="IRDLFSESSYAFTAGGHPQDDENELLASLWNAVESYEKRLFEKI
                     EKLESKIDADDMGNLKDAVLNIRKLVLENESSE"
BASE COUNT          539 a          459 c          450 g          685 t
ORIGIN      
        1 acttttcggg attgttctga atccgatcat cgacatctat ctctgtatgt acattgtatt
       61 tgcccatcat gcatattatc ttggattcaa aaaaaataaa aactattatt atctaatgta
      121 tctatggatt gggatcggct ttatcaccaa ggggcctatt tctgcagtga tcccggcaat
      181 ttcgatcggc ggggacatcc tgtacagaag ggactggaaa agacttctgg aaatgaaact
      241 tctccccgga attctgattg ccgcagttcc gcctcttctc tggtcgcttg tgcttcacga
      301 ggagttcgga agtttcggtc cttatttttt cctctggctc cagtccttcg gaagatttta
      361 taaaaagatg tacaatcaga agctggaccc tgcctatttc tacacaagtt ttctctgggg
      421 acttgcgact gtatccgtat ttctcgggat ctgtgcatgg agagaaggct gggatttttt
      481 cagcagaaac tgggctggac tccgtctcaa gactctccag aatttttcca agggaaaatc
      541 ggacggggac tatgttctgt acttctgggt gttcgtgtat ctttttctca tcggattttc
      601 cagataccag cttccgcagt atgtgtactg ggtgctgccg ggaggaatca tttacgcatc
      661 caaattcatc gaggagattt cctctgcaga gttttctttc cgttctgaat ggttttattt
      721 tatcccgcag attctttttc tgtccgcagt catcgcagtt ccgatttttg tgattgatgc
      781 agggcctgtg tattatggaa tcattatact gtatctcgtc ggattttttt tcctgaaaaa
      841 agaaaatgca tttctttcag cccttttcct tccctccgcg ctcctctttg cactcgcagg
      901 tgcggagatt tatcctgcac ttctgaaata ccagccttcc agggaaatcg gaaaaaaact
      961 gcaggagctc gaaccgggga aggacggttt ctacagtttc ggtctgcctt tttctaagcg
     1021 ttcttatgaa ttttactcag acaggatcat taagaatatt gcggacacgg acaagtttgc
     1081 agaagtcctg aaaaaggaag gaagcaggct tgtcatcact cctcaggagt atgcaatgtt
     1141 tctcgaaggg tatctcggag agagattcaa gatggaaatg gtcggagtat atcctgctca
     1201 caaagtggcc actcccacga aggaatatct tctgaaatca aagagagaaa gttctgcaaa
     1261 gaaggtgcta ttgattcggg tttcactgaa gtagaaatca tcggggaata atctcataaa
     1321 catattctcc gatattcctg ttctgaaatt cgacaccgat cagatagatg gactttcctt
     1381 tgttggtata tttttcatga tattttctgt cttttatctg ctgcagggcc tgtgccttgg
     1441 tgccgttcag cttgaattca aatatatatg tatggttatc atctatgact gcatctattc
     1501 gaccgatatt tgtcttgact tcagttttga ttttcattcc tatcagagaa aagatcagat
     1561 agaaaactgt ctgaacagtg atcagctctt caaatctgga gtaggtctct gattttgtca
     1621 gactgaagcc gtgagcactc gcaatataat ccatctgctc cttcagaaac acttccagtt
     1681 tttcggctgt accggccatc tgtttactga aatcaagacg gatgacagga tatttatgcc
     1741 acctccagtc cgaatcggcg cggacgggaa agaaaataag cgccggcagc atcagatatt
     1801 agattatata taatctcagt tttgtcaata tagaggaagt ccccttctat gatctttagg
     1861 tgtcaactga atattatagg aagtcagtct ttgtcgtttt tattcgcttg attcattttc
     1921 caataccagc ttcctgatat tcagaacggc atctttcaaa ttgcccatat catctgcatc
     1981 gattttcgat tccagttttt caattttttc gaagagacgt ttctcataag actcaactgc
     2041 attccaaaga cttgccagca gttcattttc atcgtcctgc ggatgacctc ctgcggtaaa
     2101 agcataagaa ctctctgaaa acaggtcccg tat
//
LOCUS       DAOQGJ010000601         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_34951_length_2128_cov_2.874096, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000601 DAOQGJ010000000
VERSION     DAOQGJ010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34951_length_2128_cov_2.874096"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PK683_10045"
     CDS             complement(<1..1255)
                     /locus_tag="PK683_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08832.1"
                     /translation="MKKNVFLFIIACEVFLGSGLGASDHLKEFGLKKSYAEKLFQEAG
                     IQNPPVLALTAYDIYIHAEDLYRDGDYRESRELLDKLWKKYPVGSKDWWGAKIDDSVL
                     NVGFPVCYYARLMLDEAVGWKLKEKKNPVRNAKKIQFTVILAGKSEGVQPQNLKDLET
                     KKGILKTNTLHPKLKESNFKTVHTMMWLFQEYIAAITEGKLNVKTEIVHLKNLIIPEA
                     CEKGNCSPTGDYQTRMKNELPEEILSNTDFFWLIHPSLVPDQIPEFNNMGFVTGGMGR
                     FNNSPLWISDDLSHVRKRLSKLGAPYREEEHRTYFSQWMMHEYYHYIFKLYPEFGLEK
                     ESHQWFKRNTWPADFKGQFEPDYYTEALHRRIIPKGAPLSAKLRTADRSGEYAEYISP
                     DSIEGNYEIKKVENGWHKGKIVRLSG"
     gene            complement(1711..>2128)
                     /locus_tag="PK683_10050"
     CDS             complement(1711..>2128)
                     /locus_tag="PK683_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08833.1"
                     /translation="MVHSVPPSEYVSFWTLYSGRDIPGMLSLSNSDRYPFRKEISGFS
                     TYGADLGMEFHLAPSALFDPYLLGSVGGGLCGSSCSSYRAQFGAGAKITLGERFLFFE
                     SVYEKPFFFTAGDQYYPSVESILFRFGFGLSGNAFK"
BASE COUNT          592 a          494 c          421 g          621 t
ORIGIN      
        1 tgccggaaag tcttacaatt ttccctttat gccagccgtt ctccactttc tttatttcat
       61 aatttccttc aatggaatca ggactgatgt actctgcata ttcaccgctc ctgtctgccg
      121 ttctcagttt tgcagacaga ggagctcctt ttgggataat tctcctgtga agtgcctcgg
      181 tataatagtc cggttcaaac tgtcctttga agtcagccgg ccaagtgttt ctcttgaacc
      241 attgatggct ttccttttcc agaccgaact cgggatacag tttaaatata tagtgatagt
      301 attcatgcat catccactga gaaaaataag tcctgtgttc ctcttctctg tacggtgctc
      361 ccagttttga aagtcttttc cggacatgag agagatcatc actgatccag agaggagaat
      421 tgttgaatct gcccattccc cctgtcacaa atcccatatt gttgaactca gggatctgat
      481 ccggcacaag ggaaggatga atcagccaga aaaaatcggt attggagaga atttcttctg
      541 gaagctcatt tttcattcga gtctgataat ctcccgtggg agaacagttg cctttttcgc
      601 aggcttccgg aatgatgagg tttttcaaat ggacaatctc tgtctttaca ttcagttttc
      661 cctcagtaat tgcggctata tactcctgaa aaagccacat cattgtatgc actgtcttga
      721 aattggattc cttcagctta ggatgaagag tgtttgtctt cagaattcct tttttggttt
      781 ccagatcttt taagttctga ggctggactc cttccgattt tcctgccaga atcactgtga
      841 actgaatttt tttggcattt ctgacagggt ttttcttttc cttcagtttc cagcccacag
      901 cctcatcgag catcagacga gcgtaataac acacaggaaa tcccacattc agaacactgt
      961 catcgatctt tgctccccac caatccttgg atccgacagg atattttttc cagagtttat
     1021 cgagaagctc cctgctctcc ctgtaatctc cgtccctgta aaggtcctct gcatgaatat
     1081 agatatcata tgcagtgagt gcaagtacag gaggattctg aatgcctgct tcctggaaca
     1141 gtttctctgc ataagacttc ttcagaccga attccttaag atgatcggag gctcctagac
     1201 ctgaaccgag aaatacttca catgcaataa taaataaaaa aacatttttt ttcattaata
     1261 aatctcccat gaacatctgt tctgaatgcc ggacagcgga tgaaaagatg cattccaggc
     1321 attcagcata tgaatactcg cagaatgaat taagtctgtt tttatcaatc ccaaaaaaag
     1381 aaaatcgggt gaattcgatc tatgcttcat ctgcattctg aacggcctga acaggatgcg
     1441 ttcagtccgc accggcgcag taaatctgaa atacttaaat aatttcagta tataaaatta
     1501 tataataatt caccttacgc cttcggctgc aggcggagcg ttttttggcg ggaacgcctg
     1561 caagcctggc agctgctgct tttactgatc catcagattc ctctaatgct tcgtaaaaat
     1621 tacaggttct atgcagtata ttctttttgc gcaagctgag ataataattg tctttcggta
     1681 tgcaaaggga tgatattccc ttcaaaaatc tcatttgaaa gcattgccgg aaagcccgaa
     1741 tccgaatctg aacagtatcg attccaccga aggataatac tgatctcctg ctgtaaagaa
     1801 aaacggtttt tcataaacgg actcaaaaaa aagaaaccgc tctcccagag taattttcgc
     1861 acctgcacca aactgagctc tgtaggaaga acatgagctg ccgcacagac cacccccgac
     1921 tgacccgagc aggtagggat caaacagggc agacggagct aaatgaaatt ccattcccaa
     1981 atcggccccg taagtggaaa aacctgagat ttcctttctg aacggatatc tgtcagaatt
     2041 agacagagac agcatccccg gaatatctct tccggaatag agagtccaaa atgagacgta
     2101 ttcagaaggc ggtacagaat gcaccatc
//
LOCUS       DAOQGJ010000602         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35069_length_2124_cov_3.002900, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000602 DAOQGJ010000000
VERSION     DAOQGJ010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35069_length_2124_cov_3.002900"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PK683_10055"
     CDS             complement(<1..766)
                     /locus_tag="PK683_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH08834.1"
                     /translation="MKLSQYKINNAQVVFLGGNFDMYSVKEFEEFLNGLDIQKYTRLG
                     LNFKELNSIDSSGIGALLRFGREMEKKNAEIAFYDVPGNILTIFINSGLNQIFQIMST
                     LQFENSFIKETKINSLVYKMKKKYRSFFQTIGSYRNLLAFIILALFCMTVFLTEKITY
                     DLEKSKGAELSSFIQSYLWDMDDKNAKDFAEILIETKGYRKIQINHSDGSVFIDAVSK
                     EKPSGFRFLLEQLRIIRSVSIFSDIYHKETVIGRLEI"
     gene            1254..>2124
                     /locus_tag="PK683_10060"
     CDS             1254..>2124
                     /locus_tag="PK683_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNH08835.1"
                     /translation="MIYSRLLSRTDRKQKYAFSQTMLKIIKYIFIFFMVFKAESAFSE
                     EAVQPVMVPEGSASVGLASYMEYIKDETGKLSFEEVSGTASGWKSLKGNEKVNFGYSP
                     FYYWLRFKVKNEQKERVLPIFEISFPRQDYIEFYYHDSRGNFRKKITGDTLPFSSREM
                     DHIGFAFELRTEPLSETVYYIRTRSTYNRSLLNPFLHSQNSFHKASSERNLMNGMLYG
                     LLGIMMFYNFFLFITIRERVHLYSSLTILFFSLNLSGIHGSSYQYLYPDWPWLQNVDY
                     VIFSCLTMICLILY"
BASE COUNT          650 a          432 c          408 g          634 t
ORIGIN      
        1 caatttccag tctgccgatc acagtttcct tgtgataaat gtctgaaaaa atggaaacag
       61 aacggatgat tctgagctgc tcgagaagaa agcggaatcc ggaaggtttt tccttggaaa
      121 cggcatcaat gaaaaccgat ccgtcagaat gattaatctg tatttttctg tatccctttg
      181 tttcaatgag tatttccgca aagtccttcg catttttgtc atccatatcc cagagataac
      241 tctggataaa actcgaaagt tccgctcctt tggatttttc cagatcatac gtgatttttt
      301 ccgtaaggaa aactgtcata cagaataatg ccaatataat gaatgccaga agatttctgt
      361 agctcccgat cgtctgaaaa aaggatctgt attttttctt catcttgtaa acaagactgt
      421 tgatttttgt ctccttgatg aagctgtttt caaattgaag tgtggacata atctggaaaa
      481 tctgattgag gcctgaattg atgaagattg taagtatgtt gccgggcacg tcgtaaaatg
      541 cgatttccgc atttttcttt tccatctccc ttccgaacct gagcagtgcc cctattccgg
      601 aagaatcaat ggaattcagc tccttgaagt tgagtcccaa tcgagtatac ttttgaatgt
      661 ccagaccgtt caggaattct tcaaattcct tcactgaata catatcaaaa tttcctccca
      721 gaaacactac ctgggcatta tttattttat attgagataa tttcatctga tcctctaaag
      781 actcaagggt taagaaaatc ccaagtaaaa aaaaatacaa ctgaaattaa gagcctttgg
      841 acaattttta tacaatgcaa atacgggact gcaattacaa aagtcaccca ggtccagctt
      901 gttcatcctg atataaaatt cggcgttgtc aaaattcgcg cagcggcgtc ctccaggatt
      961 cccgcagccg aggtaacgag gacagtttta cagtttattc ccggagaccc agctgcaaaa
     1021 aaggttcttg actttatgct cggcagactg gcaaaacagg aatatactac cggcgcagat
     1081 cttgcagaaa tgaactgaaa cctgcagtcc tcagcatatc aggaacagat gctgagggcg
     1141 gcggcagaaa aactgggcca acctgattac tgtcaattga gcgttgacca aaaacgtaat
     1201 tgtaaaataa ttgaatatca gtaaaaattc ggatgcataa aaataaggaa aatatgattt
     1261 acagcagact tttgagcagg acagacagaa aacagaaata tgcattttca caaaccatgc
     1321 tgaaaatcat aaaatatatt ttcatttttt ttatggtgtt caaagcagag tctgcttttt
     1381 ctgaagaagc tgttcagccg gtcatggttc cggaaggctc cgcttcggtc ggactcgcct
     1441 catatatgga atatatcaaa gatgaaacag gcaaactcag ttttgaagag gtatcaggaa
     1501 ctgcatccgg ctggaaaagc ctgaaaggaa atgagaaagt caatttcgga tattcaccct
     1561 tctattactg gcttcggttc aaagtaaaaa atgaacagaa agaaagagtt ctgccgattt
     1621 ttgaaatcag ctttcccaga caggattata tagaatttta ttatcatgat tctagaggaa
     1681 atttcagaaa aaaaatcaca ggggacactc ttcctttcag ttcaagagaa atggatcata
     1741 tcggctttgc atttgaactg agaactgagc ctctctcgga aactgtttat tatatccgca
     1801 ccagaagcac ttacaacagg tcactcctga acccttttct tcattctcag aacagttttc
     1861 ataaagcatc ttctgaaaga aatctgatga acggaatgct gtacggtctt ctgggcataa
     1921 tgatgtttta taattttttc ctgttcatca ctatccggga aagagtccat ctctactctt
     1981 ctctgaccat cctgtttttt tctctgaatc tttccggaat tcacggatca agctatcagt
     2041 atctgtatcc tgactggccc tggcttcaga atgtggatta tgtgatattt tcctgcctta
     2101 caatgatctg tctcatactg tatt
//
LOCUS       DAOQGJ010000603         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35076_length_2124_cov_2.409860, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000603 DAOQGJ010000000
VERSION     DAOQGJ010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35076_length_2124_cov_2.409860"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PK683_10065"
     CDS             complement(<1..663)
                     /locus_tag="PK683_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="HNH08836.1"
                     /translation="MEESINFVEQKYVHKTLPENNHIYNMRRALPREISIDDYEIKIL
                     PAITPAEADFLSNYYVVRETLTGKAYILKSVPQKIPKERAESLLHSAEVSSDDLEFLN
                     SMYKLNEKEGKYILVNPNITEADEVRILEAIGMKTLEERAKELGVDRFYISDYEKYEI
                     AKILEPIEGLQKKDIVFSNLFVDQKHYYFFEHPQEHLPGMFITEAGRQFVIAVCHIYG
                     NVP"
     gene            complement(1161..>2124)
                     /locus_tag="PK683_10070"
     CDS             complement(1161..>2124)
                     /locus_tag="PK683_10070"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNH08837.1"
                     /translation="EAGVPFTMKHIDEISRKIPTISKIAPASEYHIEDAHRAGGIMRI
                     LEELDLKNLLHSDAYTVSGLTLKEQIKKYSIISGENPEAEEFFKAAPGGIRTTVAFSQ
                     DRKFKELDKDREKGCVRSVESAYSKDGGLCVLYGNLALDGCIVKTAGVDEKIWVFEGP
                     AKIFQSQDDACDAILKNKIQAGDVVIIRYEGPRGGPGMQEMLYPTSFLKSKKLGKVCA
                     LITDGRFSGGTSGLSIGHVSPEAAEGGNIGLVEEGDRIFIDIPNRKIEIKVSDEELEK
                     RRKAMEAKGKDAWKPGHRPRVVSKALRAYSSMVTRAARGAIREI"
BASE COUNT          556 a          502 c          390 g          676 t
ORIGIN      
        1 agggacattc ccgtaaatat gacagactgc aataacaaac tgtctgcccg cttcggtaat
       61 gaacataccc ggcagatgtt cctgcggatg ttcaaaaaaa tagtaatgct tctgatccac
      121 aaacaggttg ctgaaaacaa tgtctttttt ctgaagacct tcgataggtt ccagtatctt
      181 ggctatttca tatttctcat agtccgaaat ataaaacctg tccactccaa gttctttcgc
      241 tctttcttcg agagtcttca ttccaattgc ttccagaatg cgaacttcgt cagcctcagt
      301 tatattcgga tttactaata tatacttgcc ttctttttca ttcagcttgt acatggaatt
      361 cagaaattcc agatcgtcgg aactgacctc cgcgctatgc aggagggact ccgcccgttc
      421 cttgggaatt ttctgaggta cactttttaa gatataggcc ttgcctgtaa gtgtttcgcg
      481 gacaacgtaa tagtttgaaa ggaagtctgc ttcagcagga gtaattgcgg gcagaatttt
      541 aatttcataa tcatcaatag aaatttcgcg aggcagtgca cgcctcatat tgtatatatg
      601 attattctcc ggcagagtct tatgaacata cttctgctcg acaaaattta tactctcctc
      661 cataccctaa aacccccata tattttatat atccttgcac ttttcaaaaa gctgctatga
      721 acaaaacggc acaggtaaat cagcgactga tcatttgttt tccattgatt cagccattag
      781 tcaatttttg aaaagagatt atttgacgat tatttctata gaattctttc tgacaattcc
      841 ttttctaggt aaaattacct aaagtgagat ttttttaaac agagcacttg aagattttga
      901 catattttca taaaaaccag atatgaaaag cggctttttg agcaatttca aaaaaaatat
      961 gcttttaata tgaagatgca gtgcttcttt ctctattttg gaaacatctg atataatgaa
     1021 aaaatattca attaaaaaaa ttacccaggg gcacccaatt gtgtattttt ttaagacata
     1081 taatgcggca ggaaaaggaa gcagagaacg aatcttctcc gcatccttca atcttctttt
     1141 acaaggtgcc gaaaggagag tcagatttct cggatagctc ctcttgcggc cctggtcacc
     1201 atggaactgt atgctcggag agctttcgat acgacacggg gtctgtggcc gggcttccag
     1261 gcatccttcc ctttggcttc cattgctttt cttctttttt cgagttcttc atcggaaacc
     1321 ttgatttcta tcttacggtt agggatatca atgaagattc tgtccccctc ttccacgagt
     1381 ccgatatttc cgccctctgc agcttcggga gaaacgtgac ctatgctgag tcctgacgtg
     1441 cctccgctga atcttccgtc agtaataagt gcacatactt ttccgagctt tttggatttc
     1501 agaaacgagg taggatacag catttcctgc attcccggcc ctcctctggg accctcatac
     1561 cggataatga caacatctcc cgcctgaatc ttattcttca gaattgcatc acaggcatca
     1621 tcctgggact ggaatatttt tgcaggtcct tcaaataccc agattttttc gtccactccc
     1681 gcagtcttca caatgcatcc gtccaatgca agatttccgt acagaacaca gagtccccca
     1741 tccttgctgt atgcactttc cacgcttctg acacagccct tttccctgtc tttgtccagt
     1801 tccttgaact tcctgtcctg tgaaaaagcg acagttgtcc ggattcctcc cggagccgcc
     1861 ttgaaaaact cctcagcttc aggattttca ccggaaataa tgctgtattt ttttatctgc
     1921 tcctttaatg taagtccgct tacagtatat gcatccgaat gaagcagatt tttcagatcc
     1981 agctcctcca gaattctcat tatcccgccg gctctgtgag catcttcaat atgatactcg
     2041 cttgcgggtg caattttgga tatagtgggt attttccggg atatttcatc aatatgtttc
     2101 atcgtgaatg gaacacccgc ctcg
//
LOCUS       DAOQGJ010000604         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35175_length_2120_cov_4.175303, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000604 DAOQGJ010000000
VERSION     DAOQGJ010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35175_length_2120_cov_4.175303"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..576
                     /locus_tag="PK683_10075"
     CDS             28..576
                     /locus_tag="PK683_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3859 domain-containing protein"
                     /protein_id="HNH08838.1"
                     /translation="MKRSAVCVFQFITGSKIKFCLLPLIFFLSVRLIQRQDPLKCEIY
                     DYGLYEYFEEHVPAENYKAVPNGEFNHFGIETKLLRRTKTFPAKVGTRIGFKYRITGL
                     PKKSKSVNISHTIRFPGFIDKNGNYFDKYSDNWESYSSDGKIEDIYGYSFDHKSELST
                     GVWTFSVWHKSKELCSISFEMQ"
     gene            760..1188
                     /locus_tag="PK683_10080"
     CDS             760..1188
                     /locus_tag="PK683_10080"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HNH08839.1"
                     /translation="MRASFFFLTPLLMTCGMLLSESAKSSRLKIRIQGVKNGKGSINI
                     ALYKTDSDFSTEKYAYVTRIPASSDTAVFEKIDPGTYGVAVFHDENENLRLDRNFAGM
                     PAEGYGFSNNAKGTFGPPSFKATAVQINQPEITLNIKLNY"
     gene            1334..>2120
                     /locus_tag="PK683_10085"
     CDS             1334..>2120
                     /locus_tag="PK683_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH08840.1"
                     /translation="MENKYDVIVIGSGIGGMTTAAILSRLAKKKVLVLEQHFELGGQT
                     HEFRRKGKFSWDVGLHYVSMMEEDSVGKKVFDYITKGQVKWNHLPYEFEKFIYPDFTF
                     TVPSNEEEYTKKLCAEFPEDSVSIKKYFQDMRKAMKWAEGEMISKLVPSFIGFFVRLW
                     NKKNESIALCTTKQYLDANFKNERLKALLVSHWGDYGLPPGKSSFLIHSIVTVHYLKG
                     ASYPEGGAGTIARAAEKIIEEAGGKCLLNAEVKGIIVRNGKAAG"
BASE COUNT          695 a          396 c          461 g          568 t
ORIGIN      
        1 agacaaaact gatgatcggg gactgatatg aaaagaagtg ctgtctgcgt cttccagttt
       61 ataaccgggt ctaaaataaa gttctgcctt ctgcctttga ttttcttcct ttcagtcaga
      121 ctgatacaga gacaggatcc tctgaaatgt gaaatttatg attacggtct ctatgaatat
      181 tttgaagagc atgtacctgc agaaaactat aaagcggttc ccaatggcga attcaatcat
      241 tttggcatag aaacaaaact tctcagaagg acaaaaacat tccctgccaa ggtcggcacc
      301 agaatcggtt ttaaatacag gatcacagga cttccaaaga aaagtaaatc agtgaacatc
      361 tctcatacga tccgcttccc agggttcatt gacaaaaatg gaaactactt cgataagtat
      421 tcagacaatt gggaatctta cagctctgac ggaaaaatcg aggatatata cggttattca
      481 ttcgatcata agtcggagct ctccaccgga gtatggacat tttccgtctg gcataaatct
      541 aaagaacttt gctccatcag ttttgaaatg cagtaaacac tgttcgcctg atgcagaggg
      601 aaagaagctt taaacgattc cgattctcaa caatgtagac agtgacaaca atttttaaac
      661 ttgcctaaaa aaattctaga ctgattagtc gtacgggaaa gactaattct ggataaggaa
      721 attacggcag ataaacctgt ctgtcttcgg gagaatccaa tgagagcttc attttttttt
      781 ctgacacccc tgcttatgac ctgcgggatg cttttgagtg aaagtgctaa aagttccaga
      841 ctgaaaatca ggattcaggg agttaaaaac ggaaaaggtt ccatcaatat tgcattgtac
      901 aaaactgact ccgatttcag cactgaaaaa tatgcatacg ttacaagaat ccccgcaagt
      961 tcagacactg cagtatttga aaaaatcgat cccggaactt acggagttgc agtatttcat
     1021 gatgaaaatg aaaacctcag actggacaga aattttgccg gaatgcctgc agaaggttat
     1081 ggattttcca acaatgcaaa gggaacattc ggaccgcctt ctttcaaagc gactgccgtc
     1141 cagatcaacc agccggaaat cactctgaac attaagctga attactgatt cgaggaagag
     1201 gttttgtatg aaaaatcatc atattttaat gattccttca aagcggggtc cgaagtactt
     1261 atctgaaagt ttgaaaagct ttttttcctt tcaacgcccc gcctgcgtaa ggtgagttat
     1321 taaggagaat taaatggaaa ataaatacga tgtaattgta atcggttccg gaatcggggg
     1381 tatgactact gcagcaattc tgagcagact ggcaaaaaag aaagtcctcg tactggagca
     1441 gcattttgag ctgggcggtc agacccatga attcagacga aagggaaaat tcagctggga
     1501 tgtaggcctt cattatgtca gtatgatgga agaggacagt gtgggaaaaa aagtcttcga
     1561 ctatatcaca aaagggcagg taaaatggaa tcaccttcct tatgaatttg aaaaattcat
     1621 ctatcccgat tttacattta cagtcccttc gaatgaagag gagtatacaa aaaaactctg
     1681 tgctgaattt ccggaagatt cagtatccat aaagaagtat tttcaggata tgagaaaggc
     1741 aatgaaatgg gcagaaggag aaatgatttc caagctggtc ccttctttta ttggtttttt
     1801 tgtaagactc tggaataaga aaaatgaaag cattgctctt tgcacaacaa aacagtattt
     1861 ggatgccaat tttaagaatg aaaggctgaa ggcacttctt gtcagccatt ggggagacta
     1921 tggacttcct cccggaaaaa gttcttttct gattcattcg attgttacag tccattatct
     1981 caaaggggct tcgtatcctg agggaggggc aggcacaatt gcaagagccg ctgaaaaaat
     2041 catagaagaa gcaggaggca aatgccttct gaatgctgaa gtaaaaggga ttatcgtcag
     2101 aaacggtaaa gctgctggag
//
LOCUS       DAOQGJ010000605         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35208_length_2119_cov_3.877907, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000605 DAOQGJ010000000
VERSION     DAOQGJ010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35208_length_2119_cov_3.877907"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PK683_10090"
     CDS             complement(<1..740)
                     /locus_tag="PK683_10090"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNH08841.1"
                     /translation="MNSSNLTNINRDTRIFGILGFPLSHSLSPTIHNTWFSEYRVNAV
                     YLVFEKEFPEKTAFFREKAFLKISGLSVTIPHKEWAFEISEECDETSKTIQASNTLVP
                     NEEGVIHSYNTDSIGALSALQKGRKNVLESKKDVLILGSGGSARGISYALLKNGYAGN
                     IIISSRNEEKAREIIGHLNQIRPASADYIQFGQLENEKKRFSLIINTTPVGMKGRTDA
                     QLLDSGFYLKTHTVFDIVYNPIRTQMVL"
     gene            complement(740..1954)
                     /locus_tag="PK683_10095"
     CDS             complement(740..1954)
                     /locus_tag="PK683_10095"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNH08842.1"
                     /translation="MYFSAKRLDVVEPSPTLAITARANALKAEGKDIVGFGAGEPDFD
                     TPQHIKNAAIRAMEKGLTKYTPTSGTVGLKDAIIDKFKKDNGLVYERKNIIAGVGGKQ
                     IIYNFFMAVLNPGDEVIIPAPYWVSYADIVRLAEGVPVIVHTTPESNFQITPDQLKKA
                     LNEKTKVFIFNSPSNPTGAAYSKKDTEALCNVLLQKKVLILSDDIYEKVIYDGLEFCN
                     PAMLSNELKERTFIANGVSKAYSMTGWRIGYGAGNPEIVKNMDTMQGQSTSNPTSIAQ
                     AAAEEALRGDQSCVAEMVKAFDDRRKKIVASVRNIPHFKCNMPQGAFYVFPYINEIYD
                     LPKFKALCAGKENEPKSKIFCDILLEKYDVAAVPGIAFGDDNAIRLSYALSQAAIDKG
                     VERIAGMVKELL"
BASE COUNT          520 a          459 c          433 g          707 t
ORIGIN      
        1 tttagaacca tctgggtccg aataggattg tatacgatgt caaaaacagt atgcgttttc
       61 aaatagaagc cggaatccag aagctgtgca tccgttctgc ctttcattcc gacaggcgtt
      121 gtatttatga tcagagaaaa ccttttcttt tcattttcca gctgtccgaa ttggatatag
      181 tcggcggaag caggacggat ctgattcaga tggccgatga tttctctggc tttttcttca
      241 tttctggatg aaattataat atttcctgca taaccatttt tcagaagtgc ataagaaatg
      301 cctcttgcac ttcctccgct tcccagaatg agtacatctt ttttcgattc cagcacattc
      361 tttcttcctt tctgcagagc agacagtgca cctatggaat cggtgttgta ggaatgaatg
      421 acgccttctt catttggcac aagtgtattg gatgcctgga ttgtcttgct tgtctcatcg
      481 cattcttctg atatttcaaa tgcccattcc ttgtgaggaa ttgttacaga aagaccggaa
      541 attttaagga aggccttttc cctgaaaaat gcagtttttt cagggaattc tttttcgaat
      601 acaagatata ctgcatttac cctgtattca gaaaaccatg tgttgtgaat cgtcggtgaa
      661 agtgaatgtg aaagagggaa tcccaaaatt ccgaaaatcc tggtatccct gttaatattg
      721 gtcaggtttg agctgttcat cagagcagtt cttttaccat tcctgcaatt ctttccacac
      781 ctttgtctat tgccgcctga gaaagtgcat aggaaagacg gattgcattg tcatctccga
      841 acgcaattcc aggaaccgct gcaacatcat atttttcaag cagaatatcg cagaagattt
      901 tgcttttcgg ttcgttttcc tttcctgcac agagtgcctt gaacttcgga agatcataga
      961 tttcattaat ataaggaaac acataaaatg cgccctgagg catattgcat ttgaaatgag
     1021 gaatgtttct tacacttgca acgatctttt tccttctgtc gtcaaaagct ttgaccattt
     1081 ctgccacgca ggactggtcc cctcgaagtg cttcctcggc tgcagcctgg gcaattgaag
     1141 taggatttga tgtggactgt ccctgcattg tgtccatatt ttttactatt tcaggatttc
     1201 ctgctccgta accgattctc cagcctgtca tcgaatatgc ttttgaaacc ccgtttgcaa
     1261 tgaatgttct ttctttcagt tcattcgaaa gcattgcagg attgcagaac tccagtccgt
     1321 cataaatgac tttttcatat atgtcgtcag aaaggatcag aacttttttc tggagaagga
     1381 cattgcataa tgcttctgta tcctttttgg agtatgcggc tcctgtagga ttggaggggg
     1441 agttgaaaat gaaaaccttt gttttctcat tcagggcttt tttcagctga tccggagtga
     1501 tctgaaaatt ggattcagga gtcgtgtgca ctatcacggg aactccttct gcaagtctta
     1561 ctatgtctgc atagcttacc cagtaaggag ccgggatgat gacctcatct ccgggattga
     1621 gaacagccat aaaaaaattg taaataatct gttttcctcc gactcccgct atgatgtttt
     1681 ttctttcata gacaagtccg ttgtcttttt tgaacttatc tatgattgca tctttcaggc
     1741 cgacagttcc ggaagtaggc gtgtatttgg tcaagccttt ttccattgcc ctgattgctg
     1801 catttttaat atgctgcgga gtgtcgaaat caggttctcc tgctccgaat cctacaatat
     1861 ccttgccctc tgctttcaga gcattcgctc tagctgtgat tgcgagagta ggggaaggtt
     1921 ctactacatc aagtcttttg gccgagaaat acatcagttt taccgatcct ttttcctaag
     1981 tctttttatt aaattatttt gctgcactca gtgcacggag ttcatccgca gcttctttga
     2041 actggtcata agtataaatt tcatattcat acccctgctc agtcaggaag agctgtctgt
     2101 tctgaccgaa tctctcctc
//
LOCUS       DAOQGJ010000606         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35224_length_2118_cov_7.274358, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000606 DAOQGJ010000000
VERSION     DAOQGJ010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35224_length_2118_cov_7.274358"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..2116
                     /locus_tag="PK683_10100"
     CDS             254..2116
                     /locus_tag="PK683_10100"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNH08843.1"
                     /translation="MNAENADRIKKQNKKEISVIIGNPPYNANQQNENDNNKNREYPL
                     IDERIKQAYIKLSTAQKTKVYDMYSRFFRWAMDRLSENGIISFVANRSFIDSRTFDGF
                     RKYIQDNFDFAYIIDTKSDVRANPKISGTAHNVFGIQTGIAILFLIRKKQDGKRKHQC
                     RIHYVSLDEYWLKEKKLLWLATNHFRDIHFEQIKPDKNNNWINIADNDFERLIPVCDK
                     EVKQGKEQKAIFRLFSLGVVTNRDDWVYDNSMTALSDKVKFLINIYNKDLEKLSGKNK
                     IEIKDGIDYSIKWTRAVKNDLAKGKKYIFEKKKIIKSLYRPFVKKFIYFSKELNEMQY
                     LNPVFFGERGTLSNELININVNGKDVSFFSSKFLSDLHFLGDSQCLPLYTYDSEGNRQ
                     ENITDLALEKFQDHYKDKKIKKENIFHYVYGVLHFPAYRKKYELNLKREFPRIPFYKD
                     FSKWRDWGKKLMGLHLEYETVKPYKLTVTHTTGKQKAEEPKAKLKADREKGIIVLDEN
                     TSLSGIPKEAWEYKLGSRSALEWVLDQYKEKKPKDPTIAEKFNTYKFADYKEQVIELL
                     KKVCTVSIETVRIVKEMAKNHDSHLKKLHRKYNQSYGQRGFKAYAQRIEYWVNE"
BASE COUNT          822 a          328 c          406 g          562 t
ORIGIN      
        1 tgccgcaggg ataggaactt tcatctgtga cctgatcgac tatataaaaa aagacagact
       61 ggaatataaa tacaaaaatg aaattcatgc aaatgagctt gcaatacttc cttactacat
      121 agccaatctg aatattgaat ttacctactc tcagaaaatg gggggataca gtgaatttga
      181 aaatctatgt ctggttgaca ctctggacaa tgcaggctgg ttttccagaa aaggagaaca
      241 gaacctgttt gtaatgaatg cagaaaatgc agacagaatc aaaaagcaga ataagaaaga
      301 aatatctgtt atcatcggaa atcctccata taatgcaaac cagcagaatg aaaatgacaa
      361 taataaaaac agagaatatc ctctgattga tgaaagaata aaacaggcct atataaagtt
      421 aagcactgct cagaagacaa aggtttatga tatgtattcc cgctttttcc gatgggcaat
      481 ggacagactg agtgaaaacg gaattatatc ttttgttgcg aacaggtctt ttattgacag
      541 cagaactttt gacgggttca ggaaatacat acaggataat tttgattttg cgtatattat
      601 agatacgaaa agtgatgtaa gagccaaccc taaaatttca ggaactgctc acaatgtttt
      661 cggaattcag acaggaatag caatactttt tttaatcagg aaaaagcagg atggaaaaag
      721 aaaacatcag tgcagaattc attatgtttc cttggatgaa tattggctga aggaaaaaaa
      781 gcttctctgg cttgcaacga atcattttag ggatattcac tttgagcaga ttaaacctga
      841 taagaataac aactggataa acattgctga taatgatttt gaaagactga ttccagtttg
      901 cgataaagaa gtaaagcagg ggaaagagca aaaggcaata ttcagattat tttctttagg
      961 agttgtgaca aatcgtgatg attgggttta cgacaacagt atgactgcct tgtcggacaa
     1021 agttaaattt ttaattaata tctacaataa agatttagaa aaactaagtg gaaaaaataa
     1081 aatagaaatc aaagacggca tagattattc cataaaatgg acacgggcag ttaaaaatga
     1141 tttagcaaaa gggaagaagt atattttcga gaagaagaaa atcatcaaaa gcctttacag
     1201 accttttgtt aaaaaattca tatatttcag taaagaatta aatgagatgc aatatctaaa
     1261 tcctgttttc tttggagaaa gaggcacatt gtctaatgaa ttaattaata ttaatgtcaa
     1321 tggtaaagat gtaagttttt tttcttcaaa atttctttct gaccttcatt ttttaggtga
     1381 ttctcagtgc ctccctcttt acacttatga ctcagagggg aatcgccagg aaaacataac
     1441 tgacttggca ttagagaaat tccaggatca ttataaagac aaaaaaatta aaaaagaaaa
     1501 tatctttcat tatgtgtacg gagtgctgca ttttccagcc tatcgcaaaa aatatgaatt
     1561 gaatctaaaa cgagaatttc cccgcattcc tttttacaaa gacttttcca aatggagaga
     1621 ttggggcaaa aaacttatgg gattgcattt agaatatgaa accgtgaaac catataagct
     1681 tacagtgact cataccacag gcaaacaaaa agcagaagaa cctaaagcaa aactcaaagc
     1741 agacagagaa aagggaatca ttgtattgga tgaaaacaca agcctttccg gaatacctaa
     1801 agaagcctgg gagtataaac tcggaagccg ctctgccctt gaatgggtat tggatcaata
     1861 caaagaaaag aagccgaaag atccgacaat tgcagaaaag tttaatacat acaaatttgc
     1921 agattacaaa gaacaagtca ttgaactgtt aaagaaagtc tgcacggtga gcatagaaac
     1981 ggtacggata gtcaaagaaa tggccaaaaa tcatgattcc catctaaaaa aactacatag
     2041 aaaatacaat caatcatatg gccagcgggg ctttaaggct tatgctcaaa ggatagaata
     2101 ctgggtaaac gaatgaca
//
LOCUS       DAOQGJ010000607         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35240_length_2118_cov_3.261270, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000607 DAOQGJ010000000
VERSION     DAOQGJ010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35240_length_2118_cov_3.261270"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PK683_10105"
     CDS             complement(<1..745)
                     /locus_tag="PK683_10105"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH08844.1"
                     /translation="MKKKIFYFFIIILSAGCLISRKILFYPVGSAAVPQIRGKIITAE
                     NGRRKVYSYFTRGNNKLIVLFHGQHGTISSSAQWAGTFASLGYSTLLVEYPGYGMAKE
                     YFASEDRIYSDSETIIKLVQTSEKFTPENTVLAGYSLGTGPAAEIALRKIGHRLILFA
                     PYTSIPDVASWRYVKILPHLIIFDRFSTASKAENIKSRTLIFHCTGDKAIPFRMSEEL
                     QKKFHNAKLIKISGGSHSILGFLNADHWNQ"
     gene            complement(772..1800)
                     /gene="fbp"
                     /locus_tag="PK683_10110"
     CDS             complement(772..1800)
                     /gene="fbp"
                     /locus_tag="PK683_10110"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNH08845.1"
                     /translation="MSRTKAKEMINLPQYLIEEQIKIPHATGDFTALMSQLVYVAKII
                     SRDVRKAGLLDNVLGSTEDTNVQGETRMKLDEYANQLLIDAMKISGHLCVMASEENDG
                     IISIPSSYHIGKYTLAFDPLDGSSNIDTNVSIGTIFSIHLRKSPNGSPGTEEDLLQPG
                     RLQRCAGYILYGSSTMMVFTMGAGVVGFTLDPSCGEFLLSHPDIKIPENGDIYSVNEG
                     NFEFWSNEFKTYIKTVKSIADNHKPKTARYIGSLVADFHRNLLKGGIFLYPEDTKSEK
                     YPKGKLRLLYEAAPMAFIAEQAGGLAVTSSGERILDMNPRELHERTTLITGSRKEVEY
                     FLKITGKK"
     gene            1904..>2118
                     /locus_tag="PK683_10115"
     CDS             1904..>2118
                     /locus_tag="PK683_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08846.1"
                     /translation="MIYDSELKNKFFFQPSFFSKHLLLILIVIPFWQCSKQSSNTAVI
                     LSDPKGAQVFSEKGELLGNTPLLLKNIS"
BASE COUNT          554 a          448 c          433 g          683 t
ORIGIN      
        1 tttggttcca gtgatctgca ttcaggaatc cgaggatgga atgacttccg cctgatattt
       61 taatgagttt tgcattatgg aattttttct gcagttcctc gctcattctg aaggggattg
      121 ctttgtctcc tgtgcagtga aagatcagag ttcttgattt tatgttttcg gccttcgatg
      181 ctgttgaaaa tctgtcaaat atgatcagat gaggaagtat ttttacatac ctccagcttg
      241 cgacatccgg gatggaagta tagggtgcaa acagaatcag cctgtgaccg atttttctga
      301 gggcaatttc tgccgcaggc cctgttccca gagaataacc cgcaagcaca gtgttttcgg
      361 gagtgaattt ttcacttgtc tggacaagtt taataattgt ctccgaatca ctgtatattc
      421 tgtcctcaga agcaaagtat tcttttgcca tcccgtagcc gggatattct acaagcagag
      481 tggaatatcc gagtgaggca aaagttccag cccactgtgc ggagctgctg atggttccgt
      541 gctgtccgtg aaagagaaca atcagcttat tgtttcccct tgtaaaatag gaatagactt
      601 tgcgtctgcc gttctctgcg gtgatgattt tccctcgtat ctgaggaact gcagccgaac
      661 cgacagggta gaaaagaatt tttcttgaga tcagacatcc tgccgatagg ataatgataa
      721 aaaaataaaa aatctttttt ttcatcggca ggacagatat atttttccgg actatttttt
      781 ccctgtaatt ttcagaaagt attccacctc ttttctgctt cctgtaatca gtgttgttct
      841 ttcgtgaagc tccctagggt tcatatcgag gattctttca ccggaggaag tgactgcaag
      901 tcctcctgcc tgttctgcaa tgaatgccat aggagcagct tcatacagaa gtctcagttt
      961 gcccttggga tatttttccg actttgtatc ttcaggatac agaaatattc ctcctttcag
     1021 aaggtttctg tgaaaatcag cgacaagaga tcctatgtac cgggctgttt tcggtttatg
     1081 attgtccgca attgatttta ctgtctttat gtaagttttg aattcattgg accagaattc
     1141 aaagttgcct tcgttcactg aatagatgtc cccgttttcc ggaattttaa tgtccggatg
     1201 agaaagaaga aattctccgc aggaaggatc aagagtaaat ccgaccactc cggctcccat
     1261 tgtaaatacc atcattgtag aagatccata taatatgtat cctgcacatc tttgaagtcg
     1321 gccaggctga agcagatctt cctcagtccc gggcgagccg tttggcgact ttctcagatg
     1381 tatcgaaaaa atggttccga tggatacatt cgtgtctata ttcgaggaac cgtccagagg
     1441 atcaaaagca agcgtgtatt ttcctatatg ataactgctc ggaatgctga ttatcccgtc
     1501 gttttcctca ctcgccatca cgcagaggtg tcctgaaatt ttcattgcat caatcaggag
     1561 ctggttcgca tactcatcga gtttcattct agtctcgccc tgaacgtttg tatcttcagt
     1621 agatccaagc acattgtcca gcagtcctgc cttccttaca tctctcgata taatctttgc
     1681 aacatagacc agctgactca tcagtgcagt aaagtcccct gtggcatgag ggatttttat
     1741 ctgttcttct atcagatact gaggaagatt aatcatttct tttgcttttg ttctgctcat
     1801 gaaactaata tttatttcat tactgaaaat tcaagaattc taaatcgaaa aaagagaatc
     1861 tgctttactg aaaacaggaa attctgtaaa atgatacacc gatatgatct atgattctga
     1921 attaaaaaat aaatttttct ttcaaccttc attcttttcg aagcatcttc tgctgattct
     1981 gattgtcatt cccttttggc agtgttcaaa gcagtcttcc aatacagcag tcattttaag
     2041 tgatcctaaa ggggcacagg ttttctcgga gaaaggcgaa cttttgggaa acacacctct
     2101 cctgctgaaa aatatttc
//
LOCUS       DAOQGJ010000608         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35244_length_2118_cov_2.840039, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000608 DAOQGJ010000000
VERSION     DAOQGJ010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35244_length_2118_cov_2.840039"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1566)
                     /locus_tag="PK683_10120"
     CDS             complement(271..1566)
                     /locus_tag="PK683_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNH08847.1"
                     /translation="MKKSKLFSTMWVLTSMLLGLTFCPGVDENEKNQNSKKAVAAAVV
                     VTSGRSTSGASSSSAAPSGLSYSGSPFSFAQGIAITAVTPAMTGTATSCTSSPTLPTG
                     LSINGTTCAISGTPTTLQSATNYTITAANSAGSTTATISISVTSNGSTWTARTMSSSQ
                     FWTSVTYGNGLFVAVAQGPSTVASTSPDGITWTARTLPSSSDWISVTYGNGVFVAIAA
                     NSTAAASSTDGITWTARTLPSSSQWTSVTYGNGLFVAVAGVSNAAASSPDGINWTART
                     LPSAAGANTVTYGNGVFAGIATGSTAASSSTDGITWTARTMPSSSNWSSITYGNGVFV
                     AVSMTFGTVAATSADGITWTARTMPSSQSWKAVTYGNGVFVAIATGPSTVAATSTDGI
                     TWTVRTLPSNSGWQCVTYGNGIFVATAGMTTSSAAATSP"
BASE COUNT          609 a          484 c          458 g          567 t
ORIGIN      
        1 ataaaatcat tagattaaaa ataatccaaa caaattcttc ttgagcccaa aaatatagca
       61 ttcaggagaa acttgtaaat ctaacagagt tgttgaggga aattcagtcc aaggtttcag
      121 caaaaagcac gattttttac atcatttgaa attttttttt gaaagaatct attttttgca
      181 aggatgtaca gcagtacatc cttcgggtga aatttaactt ctgcgcaatc gtaaacgatt
      241 cttctgcaag aagattatcc ttaagcttta tcagggagaa gtcgctgcag cagaagatgt
      301 tgtcattcca gctgtggcca caaaaatacc gttcccgtaa gtcacacact gccagcccga
      361 gttgcttggc aaagttcgga ccgtccaggt aattccgtca gttgatgttg cagcaacggt
      421 tgagggtccg gttgctatgg ccacaaaaac accgttccca taggttactg ctttccacga
      481 ctgtgaactg ggcatagttc tggccgtcca agtaattcca tccgcagacg ttgcggcaac
      541 tgtgccaaat gtcatcgaca ctgccacaaa aacaccgttc ccataagtta ttgaactcca
      601 gttggaagaa ctgggcatgg ttctggccgt ccaggtaatc ccatccgttg aacttgatgc
      661 tgctgtggaa ccagtagcaa tcccagcaaa tactccattt ccgtaggtca ccgtattggc
      721 tccggcagca cttggcagag ttctagctgt ccagttaatt ccatccgggg aacttgccgc
      781 tgcattcgaa acgccggcta cagctacaaa aagaccgttc ccgtaagtca ctgatgtcca
      841 ctgcgaagaa gagggcaaag ttctggctgt ccaagtaatt ccgtcagtgg aacttgccgc
      901 tgcagttgag ttggcggcga tagccacaaa aacaccgttt ccgtaagtca ctgaaatcca
      961 atctgaagaa cttggcaagg ttctggctgt ccaggtaatt ccatcaggtg aagtggacgc
     1021 cactgtcgaa ggcccctgcg caacagcgac aaaaagaccg ttcccgtaag tcactgatgt
     1081 ccagaattga gagctggaca tagttctggc tgtccaggtg gagccatttg atgtgactga
     1141 aatactgatt gtcgcagtcg tacttcccgc tgaatttgca gctgtgatag tataatttgt
     1201 cgcagactgc aaggttgtag gagttccgct gattgcacag gtcgtgccgt tgatactgag
     1261 cccggttgga agtgtcggac tggaagtaca gcttgtcgca gttcctgtca tagcaggtgt
     1321 aacagctgta atggctatgc cctgagcaaa actgaatgga ctgccgctgt aactcagacc
     1381 cgaaggtgca gctgatgatg atgacgctcc tgatgtactt ctgccagaag ttacaacgac
     1441 agcggcggcc actgcctttt tactgttttg atttttctca ttttcatcta ctcctggaca
     1501 gaatgtcagt cccaacagca ttgaagtcaa tacccacatt gtggaaaaga gtttactctt
     1561 tttcatacaa aaatactcct atattaaagt tttcaatgga atagattcat agagccgcca
     1621 cctcctaatt ttatgcataa ttcaatattc agtgattttt tatttttttg cttaaaatcc
     1681 gaaaaacctt aggggaaccc ctaaacgttt ggggaattca acggattcgc cgcagactca
     1741 ctccgttttt tataaaacac tgtgcaacaa cttcaccctc cttctccatc cacacagaat
     1801 gatcactcga acatatggac agtatcttca agaataaatg gcaaagctca gtattgacca
     1861 aaaacaattt cttgaagatg aatccaaacg tgcagaacag cgatgaaaat ttttcagatg
     1921 gatcagcaca aaaatttaga caaaaaattc ggggaaaaaa tctaagctaa aaaagtcagc
     1981 acggaggtag tattagcatg aactggaact ttataaagga ttcgatcctg aaagacgatt
     2041 ttatcatcga ctgccgtctt caggcatcct acgaagaaga gactgcctct ggtgctcatt
     2101 tctttacgtt catcaaga
//
LOCUS       DAOQGJ010000609         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35411_length_2112_cov_2.749635, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000609 DAOQGJ010000000
VERSION     DAOQGJ010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35411_length_2112_cov_2.749635"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PK683_10125"
     CDS             complement(<1..1519)
                     /locus_tag="PK683_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH08848.1"
                     /translation="MKKPDMIQLPGIEIIEKIYEGGKNVIYKALKNQKKIIIKTSKYI
                     YPKLGEVKRLVREYEILSRFRSEGIVRPLELIDFQNRPFLVMEDIDGVSLDQLILQQN
                     LQIGFFLDLALKTVNAVSEIHKNHIIHKDIKPHNIIYNMQTGELQIIDFENASLFEEE
                     LSESNEEFFEGSMAYMSPEQSGRMNRSADYRSDYYSLGVTFYQLLTSELPFRTDDAMK
                     IFHAHLAQKPESPKEKNQTPVIISDIIMKLMEKTPESRYQSSYGILKDLLKCRQAMDA
                     MIPEDFKNFGFPLAQYDGFSEFKIPKKLYGRKKEIENLLNAFSQVRLQGGKHLTLISG
                     YSGVGKSALTMELQKPVTSSGGYFLTGKFDQLNSNKPFYAVIQVFSKLITLLLGENSE
                     NSSLWRERIVSALGRNGKLLTDLIPELEIIIGKQPDVSASSSLENANRFNLLFINFLK
                     VFTQKENPITVFLDDMQWADRSSLNLLLNILQEPSLNWFCLILAYRKNEVSDSHPF"
BASE COUNT          629 a          436 c          389 g          658 t
ORIGIN      
        1 aaaacggatg actgtcagaa acttcatttt tgcggtatgc cagaatcaga cagaaccagt
       61 tcagtgaagg ctcctgaaga atgttcaaaa gcagattcag agatgatctg tctgcccact
      121 gcatatcatc cagaaaaaca gtaatcggat tttctttctg cgtaaatact ttaagaaaat
      181 ttataaaaag aagattaaat ctgttcgcat tttccagtga actggatgca gacacatcag
      241 gctgcttgcc tattataatt tcaagttcag gaatcagatc tgtcagaagt tttccattcc
      301 ttcctaaagc ggagacaatt cgttccctcc agagagaaga gttttcactg ttttctccga
      361 gaagcagagt aatcagcttt gaaaatacct ggatcacggc ataaaaaggc ttattcgaat
      421 ttaactgatc aaattttcca gtaagaaaat atccgccgga tgaagtcact ggtttttgaa
      481 gttccattgt cagtgcggat ttaccgacac cggaatagcc tgaaatcaga gtcaagtgtt
      541 tgcctccctg cagccgaacc tgagagaaag cgttcagaag attttcaatt tccttctttc
      601 tgccgtacag ttttttcggg atcttaaact cggaaaaacc gtcatactga gccaatggaa
      661 agccgaaatt cttaaaatct tcaggtatca ttgcatccat tgcctgcctg catttcaaaa
      721 gatccttgag aattccgtac gaactctgat atctgctttc aggagtcttt tccataagtt
      781 tcattataat atctgatata ataacaggag tttgattttt ttcctttgga gattccggtt
      841 tttgggcaag atgagcatga aaaatcttca ttgcatcatc tgtccgaaac ggcagttctg
      901 aagtcagaag ctgatagaat gtcacaccga gcgaataata gtctgatctg taatctgcac
      961 tgcggttcat tcgtcctgac tgttcagggg acatatatgc catgcttcct tcaaagaatt
     1021 cttcattgga ttcagataat tcctcctcga ataatgaagc attctcaaaa tcaatgatct
     1081 gaagttcacc tgtctgcata ttgtatatta tattatgagg ttttatatct ttatgtataa
     1141 tatgattttt gtgtatctcg gaaaccgcat ttacagtttt cagtgccaga tcaagaaaaa
     1201 aaccaatttg aaggttctgc tgcagtatga gctgatcaag agagactcca tctatatcct
     1261 ccattacaag gaaaggcctg ttctgaaaat caatcagttc taaagggcgg acaattcctt
     1321 cagaacggaa cctgctgagg atttcatatt cacggacaag tcttttgact tcgccaagtt
     1381 tcggataaat gtattttgat gtttttatga ttattttttt ttgatttttt aatgctttat
     1441 atattacatt ttttccgcct tcatatattt tttcgattat ttcaattcca ggaagctgaa
     1501 tcatatcggg tttcttcaca ttaatcagac ttcattgcag cagctgcaga aaaggccaga
     1561 ggaaacactg ttccggcaaa caagactatc tgttgaacgg tcccgactct gcgagactct
     1621 tctggaattt ccgaggaaaa taaattctta aactgtttcc agtgaactcg ccagtcaggc
     1681 ataaaacccg ttcctgcaac ccattccaga atcggaagtt gagaagatat ttttaaacca
     1741 ggatcgacca ttcggtcaat ctcacaggca gtcctatata ccgagtcgac tgacttaaca
     1801 atatgcttcc tcgtataaac aatgtacatt catatttcct ttgcaggatc gtgtacgaca
     1861 ctaagaaact gctttggcat catagatacg cttaaatttt atcctccccg ctgatgcagg
     1921 caagcgaaat attccatcag tttctcagat ttagaaaacc ggcaagcagc ggaaatttcc
     1981 cgtaaaaaat aaatgcacct attatcattc ttttgatatc tgcatcgaac aaataggggt
     2041 tttccggatt cagtccaacc atatcgaaaa gatttccttt tgcaggactg ccatacttac
     2101 ttaccggata gt
//
LOCUS       DAOQGJ010000610         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35437_length_2111_cov_3.223249, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000610 DAOQGJ010000000
VERSION     DAOQGJ010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35437_length_2111_cov_3.223249"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..893)
                     /locus_tag="PK683_10130"
     CDS             complement(36..893)
                     /locus_tag="PK683_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08849.1"
                     /translation="MDAEYRKSVFENENTYIFKRSSVFKDLPVYGFAEFLNSVFLFAG
                     LKERKAVLEKSNTFAYICTYYECRTEVKGTVLSIHTYPGMKLKSFDPAHYSSVKKMTE
                     RIQLNTEMIHNGISFAVFAIDEKGFHLNIQDAVLKSYGKGTLQFNSRMQVSLFGMNIY
                     MNGLKYRIEYSQNDRTESHIQGRYEKTDPEIKTAGSILSNAVDILIPGNIEGYFRMYF
                     RQAMFGNSKKGNNFEGKSEVKKGRMNIYMSSESEIPRNPFRFLAGRNSEKNAPVFMDG
                     LESAVLKDL"
     gene            complement(1181..>2111)
                     /locus_tag="PK683_10135"
     CDS             complement(1181..>2111)
                     /locus_tag="PK683_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH08850.1"
                     /translation="NRDNLNRDKKRKTVGRPYAYVKVSDGCNRGCHFCIIPNLRGNFR
                     DQSEDGILLDAQSAVSAGAKEICIVSQDTVFYGKDTDRLKKVLDQISGINGLELLRLL
                     YLYPDKKTYNLLDYFKENDKLAPYLESPIQHVSEKMLKRMNRSGSYSFFADLFGKARE
                     ARKGLEIRTSLILGYPGETEDDLEETVRFIKEVKPEKLALFAFSPQEGTKAAELENDI
                     SEEEAARRVNYVREVHLEVLKDIHQDRIGKIYPAIVDEVSKDQILVRRFQDAPEIDEI
                     VFTPFRKDLSVGQIGKVKIGSFLEYDMEGEFIH"
BASE COUNT          581 a          459 c          372 g          699 t
ORIGIN      
        1 caagaataat cgagggcttc agaacaggat ccaaattaca gatcctttaa gactgccgat
       61 tccagcccgt ccataaagac aggagcattt ttttctgaat ttctgccggc aaggaaacga
      121 aacggatttc ttggaatttc agattctgaa ctcatataaa tattcattct tccctttttt
      181 acttcagatt taccttcaaa attattgcct ttttttgaat ttccaaacat agcctgtctg
      241 aaatacattc tgaaatatcc ttcaatattg cctggaatca gtatatctac cgcattgctc
      301 agaatgcttc ctgcagtctt tatctccgga tcagttttct catatcttcc ctgaatatgc
      361 gattctgttc tgtcattctg actgtattcg attctgtatt ttaagccgtt catataaata
      421 ttcattccga aaagagaaac ctgcattctg gaattgaact gcagagttcc tttaccgtaa
      481 gacttgagta ctgcatcctg gatattgaga tgaaagccct tttcatcaat tgcaaaaaca
      541 gcaaaactga tcccattgtg aatcatttca gtattcaact ggatccgttc agtcattttt
      601 tttacggagc tgtaatgtgc aggatcaaag gatttgagtt tcattcctgg atatgtatga
      661 attgatagaa cagtgccttt tacttcagtc ctgcattcat agtatgtaca gatataggca
      721 aatgtattag atttttctaa tacagctttt ctttctttta agcctgcaaa caggaatact
      781 gaattcagaa attctgcaaa cccgtagaca ggcagatcct tgaagactga tgacctttta
      841 aagatataag tattttcatt ttcaaaaacg gattttctgt attctgcatc cactctgaat
      901 ttaaaatttg aatttcgatt ttccggtttt cctaatgtga tgtaattctc atttgtgttt
      961 tcggcggata ggaaataatt ttctgatcct ctgaaatcct tttcaaattc ctcccgaaaa
     1021 ccaccgttca gaaaagtcca ttcctttctg ttgaaaaagc tttttctgaa acttttctgg
     1081 atttgaaagt attccgtcca aaaattcttg ccagatgaac tgactctgca gtccgaaaat
     1141 aaaaagaaga taatgaatgg aaaaagaacc tttaaagctg tcagtgaatg aactcccctt
     1201 ccatatcgta ttccaaaaaa gaaccgattt ttacttttcc aatctgaccg acagacagat
     1261 ctttcctgaa cggtgtgaat acaatttcat caatttcggg agcatcctga aatctcctta
     1321 caaggatctg atccttagaa acctcatcca cgatggccgg gtaaattttt ccgatcctgt
     1381 cctgatgaat gtctttcagt acttcgagat gcacttcacg gacgtaattc acccgtctag
     1441 ccgcctcttc ttcagagata tcgttttcga gttctgctgc tttggtgcct tcctgcggcg
     1501 aaaaagcaaa tagagccagt ttttcgggtt tgacttcctt tatgaaccgg acagtctctt
     1561 ccagatcgtc ctcagtttca ccgggatatc ccagtatcag tgaagttctg atctcaagtc
     1621 cttttctcgc ctctctggct tttccaaaaa ggtccgcgaa aaaagaatag ctgcctgatc
     1681 tgttcattct tttaagcatt ttttcagaaa cgtgctgaat cggtgattcc agatagggcg
     1741 ctaatttatc attttcttta aaataatcga gcagattata tgttttctta tcaggataaa
     1801 gatagaggag acggagcagc tccagaccgt ttattccgga gatctgatcc aggactttct
     1861 tcagacggtc tgtatccttt ccatagaaga cagtatcctg agatacaatg catatttcct
     1921 ttgcaccggc agaaacagct gactgcgcat ccagtagaat tccatcctcc gactgatctc
     1981 tgaaatttcc tctcagattg ggaatgatgc agaaatgaca gcctcgattg catccgtcag
     2041 acactttgac ataggcatac ggtctgccga ctgtctttct tttcttgtcc ctgttcagat
     2101 tgtctctgtt g
//
LOCUS       DAOQGJ010000611         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35438_length_2111_cov_3.042802, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000611 DAOQGJ010000000
VERSION     DAOQGJ010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35438_length_2111_cov_3.042802"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PK683_10140"
     CDS             <1..682
                     /locus_tag="PK683_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNH08851.1"
                     /translation="GYIKLSGFIFDSAFLKTASTLGFPSAVDGVLTHSSFLIFTKIAG
                     LISVTSVAGVNIVLTIMMISFMPGIAFGIAGTTLFGQSAGAKKWNRAEISIYRSANYA
                     LLIMGTMGLIFIIMSRQIISLFTDSAAVILDTVPCLFVMGLAQGADAYHMTFGAALRS
                     AGMVNWVLIVYLIASYAVMLPIAWILGIYFSLGSIGLWISVAVWLYFLGIVFFLKFRK
                     KEWKGVSF"
     gene            844..>2111
                     /locus_tag="PK683_10145"
     CDS             844..>2111
                     /locus_tag="PK683_10145"
                     /inference="COORDINATES: protein motif:HMM:NF025563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HNH08852.1"
                     /translation="MKKNRIWVLIILAGCSAGVKIPKSAVSAEIKPESANKFLNLANG
                     KSLEVKGISTENGALVQQSAYVGSESQRWTLERKGIDEYRLKNVFSKKYLTEENSGTL
                     TQQDSNPSLSQNWNFVPFENNYITIKNVKSQLYLSGNSSAKDNLPFQLLKSEEKEIQF
                     WKMETVPVQKPVVFKVLLIIKKKTDLNPPYVSKPYRAEMDQEYINAAKISFMEHTPYW
                     VDKLTEGRMKWEPETVVSEEPLTRLARSVKKDDGWKVPSAEDMEDEIRKYAVKGKYDI
                     IFLNYYIDLDKGGGGTAYGTPDIANKTGFAVVNRLYTGELLKKTAGVHDMWIHEGTHC
                     IEMHYLYDRGYKYGIQGGADGGGSHGYPNDTVHGWREFYYNYFKGTVPENGELRGLGE
                     RAWAYGNIRDWVINRNTKTMEDILRNDSSFS"
BASE COUNT          650 a          380 c          494 g          587 t
ORIGIN      
        1 gggatacata aaactttccg gattcatctt tgattctgca tttctgaaga cagcatccac
       61 tctgggtttt ccttcggctg tggacggagt tctgactcac agttcttttc tgatcttcac
      121 taagattgca ggactcatca gtgtgacttc agtggcaggt gtgaatattg tgctcacaat
      181 catgatgata tcgtttatgc ctggaatcgc attcggaatt gcaggcacga ccctgttcgg
      241 acagtccgcc ggagcgaaga aatggaaccg tgcggagatc agtatctaca gatccgcaaa
      301 ttatgcgctt cttattatgg gcacaatggg tctgattttc attattatgt ccagacagat
      361 aatatcatta tttacagaca gtgctgcagt catactggat actgttccct gcctttttgt
      421 aatgggactg gcacagggag cagatgccta tcatatgaca ttcggagcag ctctcagaag
      481 cgccggcatg gtcaactggg ttctgattgt atatctgatt gcatcttacg cagttatgct
      541 tccgatagcc tggattctcg gcatatactt ttctctcggt tccatcggac tgtggatctc
      601 agttgctgtc tggctctatt ttctagggat tgtttttttc ctgaaattca gaaagaagga
      661 atggaaggga gtgagtttct gataatcaaa aactgcaatt ttaaaaaatg agaatcggaa
      721 aaatttgcat atttttcatt tcctgataat ataaaaatct attactggga cttccaatgc
      781 tgaaaatgac agcgatgcgg catagcgtgg ttgcaagtct gagcaatcac tcaggagtat
      841 tgaatgaaga aaaatcgtat ttgggtactg attattttgg ccggatgttc tgccggcgtg
      901 aaaattccga agagtgcggt ttcggctgaa ataaagccgg aatctgcaaa caagtttctg
      961 aaccttgcaa atggaaagtc tttggaagtg aagggaattt ctacagaaaa tggtgcactg
     1021 gtgcagcagt cggcttatgt aggcagtgaa agtcagagat ggactcttga aaggaagggc
     1081 attgatgaat atcgcctcaa aaatgttttc tcaaaaaaat atcttacaga agagaattcc
     1141 ggaacactga ctcagcagga ctcaaatcct tctctttctc aaaactggaa ttttgtacca
     1201 ttcgaaaata attacataac gataaaaaat gttaaaagtc aattatatct ttcagggaat
     1261 tcttccgcaa aggataatct gccttttcag cttttgaaaa gcgaagaaaa ggaaattcaa
     1321 ttttggaaaa tggaaactgt tccagttcaa aaacctgttg tctttaaagt ccttctcatc
     1381 atcaagaaaa aaactgatct gaatcctcct tatgtttcca aaccataccg tgcagaaatg
     1441 gatcaggaat atataaatgc agcgaagatt tcctttatgg aacacactcc ttactgggtg
     1501 gataaactga ctgaaggcag gatgaaatgg gaaccggaga ctgtcgtttc cgaggaacct
     1561 ctgaccagac ttgcacgatc agttaaaaag gatgacggat ggaaggttcc ttctgctgaa
     1621 gatatggaag atgaaatccg gaaatatgca gtaaaaggga agtatgatat aatattttta
     1681 aattattata ttgatttgga taaaggtggg ggaggaactg cctatgggac tcctgacatc
     1741 gcaaacaaga caggattcgc tgtggtaaac agactgtata ccggtgagct tctgaaaaaa
     1801 actgcaggtg ttcatgatat gtggatacac gagggaactc actgcattga aatgcattat
     1861 ttatatgaca ggggatataa atatggaata cagggcggag cagacggagg aggcagtcac
     1921 ggatatccga atgacactgt tcacggatgg agagaatttt attataatta tttcaaagga
     1981 acagttccag agaacggcga actgagggga ctcggcgaaa gagcctgggc ctatggaaat
     2041 atcagagact gggtgatcaa cagaaataca aaaacgatgg aagacattct cagaaatgac
     2101 agcagctttt c
//
LOCUS       DAOQGJ010000612         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35499_length_2109_cov_3.384615, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000612 DAOQGJ010000000
VERSION     DAOQGJ010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35499_length_2109_cov_3.384615"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="PK683_10150"
     CDS             complement(<1..1673)
                     /locus_tag="PK683_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08853.1"
                     /translation="MKIFHQIAFGLFLFLLTHVLLYSFADRTNPRQHLYYYPNGYIVN
                     SNVQNQNFLGFNIFHDVKFPKFDLGIEPEDYDFTKSLLNKSEFDFIPHIFGSAVFFMI
                     AVWFFRTSGDILISYMMLLLALLITSDMYLLCYGSVLWLFYFILFQIGFLILHLSVRL
                     KGRDIPSKWMVPEIAFSALIAYFGNTSSSDPGVFTRMVTMAGGMILFFSLSAFLVSIY
                     DLVKYRQDDTNKTNKLLVAAGILSVISMQYVIYSTNLLKGSPVLRYSIYFFYLIFPLT
                     FIYSSYRYQVLPQQILFNSSLTSIFLFLISVVSYFVLYSAVIFISPSAQGKNEGLSYE
                     SFSIFNYIFLFITVFYIYTLKKYLSDLIDRWTFSRNPRLKESLQEFTNLISSRISLKD
                     TVRTISERIAEALQVEKVIMLVPAYQFPKYNFKDLKSIMKIQPTSEVWNYFSRQKGFT
                     VSSYLQYGAGSREHTMRFLNDLKIQLSYTIMHPETDRLNAVFLLGEKKNRKGFTIGEI
                     FFIKECSRIANLLLQNYLLLNSEIEKKKMQREFTTAEIINNTLLPPVFDS"
     gene            complement(1670..>2109)
                     /locus_tag="PK683_10155"
     CDS             complement(1670..>2109)
                     /locus_tag="PK683_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08854.1"
                     /translation="NTAGCLLESIEFLDKHRIFILLSLEGNKRFGASLVLSHILGKII
                     GSGFKNDRAFPHKIIKNFIEHELGEAGWNDGYDLLIIGFSEASHEMVFSRIGGRFRVT
                     EGSPPKLIPVRWRKVLTAGTEPITVFRGIQPLVRITAETGKES"
BASE COUNT          682 a          423 c          435 g          569 t
ORIGIN      
        1 gaatcaaaaa caggaggaag gagagtattg ttgatgatct cagcagtcgt aaactctctc
       61 tgcattttct tcttttcaat ctctgaattc agaagaagat aattctgaag aagcaggttt
      121 gctatacgtg aacattcctt aatgaaaaat atctctccga tagtaaaacc tttcctgttc
      181 tttttttctc cgagcagaaa cactgcattc agtctgtccg tttcagggtg catgattgta
      241 taggaaagct gaatcttcag atcattcaga aaccgcatcg tgtgctctct tgaaccggct
      301 ccatactgaa gataggatga aacagtgaac cctttctgtc tggaaaagta attccacacc
      361 tctgaagtag gctgaatctt catgatgctt ttcagatcct tgaaattgta ctttggaaac
      421 tgataggctg gcacaagcat gatgaccttt tccacctgaa gtgcttctgc aatccgttcc
      481 gagattgttc ttacagtatc cttcagagaa attcttgaag agatcagatt cgtaaattcc
      541 tgcagacttt ccttcagtct gggattcctg ctgaaggtcc atctgtcaat cagatcactg
      601 agatattttt tcagagtata tatataaaaa acagtaataa aaaggaaaat ataattaaaa
      661 atggaaaaag actcgtatga gagtccttca tttttcccct gtgctgaagg actgatgaat
      721 atcactgcag aataaagaac aaaataggaa acaacgctga tcagaaaaag aaaaatgctt
      781 gtcagagacg aattgaaaag gatctgctga ggcagaacct gatatctgta agaagaataa
      841 atgaatgtca gagggaagat aagatagaaa aaatagatgg aataacggag gaccggactt
      901 cctttcagaa ggttcgtaga ataaatgaca tactgcattg aaatgacaga cagaatccct
      961 gccgcaacca gaagcttgtt tgtcttgttt gtatcatcct gtctgtattt gacgagatca
     1021 tagatgctga caaggaaggc agacagggag aaaaacagta tcatccctcc agccatcgtc
     1081 accattcggg tgaacactcc aggatcggag ctcgatgtat ttccaaaata ggcaatcagt
     1141 gcagaaaatg cgatctcagg aaccatccat ttcgaaggga tgtccctgcc tttcagccgt
     1201 acacttaagt gaagaatcag aaaaccgatc tgaaaaagaa tgaaatagaa aagccagagc
     1261 accgaaccgt agcataaaag gtacatatcg gatgtgatca gcagggcaag aagcagcatc
     1321 atatacgaaa tcagaatgtc cccggaagtt ctgaaaaacc agactgcaat cataaaaaag
     1381 accgccgaac cgaagatatg aggaataaaa tcaaactcag acttgttcag cagggacttt
     1441 gtaaaatcgt agtcttccgg ttctatgccc agatcaaatt taggaaattt cacgtcatga
     1501 aaaatattga acccgagaaa attctgattc tgcacatttg aattcacaat gtagccgttc
     1561 ggataataat aaagatgctg tcttggattt gtcctgtcgg caaaactgta gagaagcaca
     1621 tgagtcagaa gaaaaagaaa cagtccgaag gcaatctgat gaaatatctt caagattctt
     1681 ttcctgtttc agcagtgatt cttacaagag gctgaatccc tctgaaaaca gtgatcggtt
     1741 cagtgcctgc agtcagcact tttctccatc tgactgggat aagtttcggc ggacttcctt
     1801 cggttacgcg gaagcgtcct cctattcttg aaaacaccat ctcatgactt gcctcgctga
     1861 atccgattat aagcagatcg tatccgtcgt tccaacccgc ttctccaagc tcatgttcta
     1921 taaagttttt tataatcttg tgcggaaatg ctctgtcatt tttgaatccg cttccgatga
     1981 tttttcccag aatatgggaa agcacaagag atgcaccgaa tcttttgttg ccttcgagag
     2041 aaagaagtat gaaaatcctg tgcttgtcaa gaaattctat ggattcgaga agacatcctg
     2101 cagtatttg
//
LOCUS       DAOQGJ010000613         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35533_length_2108_cov_2.856308, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000613 DAOQGJ010000000
VERSION     DAOQGJ010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35533_length_2108_cov_2.856308"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..585
                     /locus_tag="PK683_10160"
     CDS             193..585
                     /locus_tag="PK683_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08855.1"
                     /translation="MFAMRKRRFFISGKKIKFLAFLCLFSFLSFTDNGLKSIGGSSSA
                     LKPMIRLSSVPVHRQSSELLENTSVTSESFLSEETEDDFEIIAFFLSENISYSILPSS
                     MRYARGGMEYQKISLHYTYTDLPPPAKV"
     gene            611..>2108
                     /locus_tag="PK683_10165"
     CDS             611..>2108
                     /locus_tag="PK683_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNH08856.1"
                     /translation="MLKFLKFIIFAELFCSAGTALYLMTLPNPDAVSIIGLGLLSILG
                     LPIYFSSGIFEDKGRPSRHIGFILFFAGQFVCLISGKSIWLPVFWELSSIGCLFFYPD
                     ENLLKKSSKSASAFLLSSGLSMFFISLWIFLPDSNFGRTSLLIGLLIKAAFSGFHVWY
                     PIAHEGAPYYASASISGIMMNLPMLMIVKFLYADLHTMNVTTLIMILCGLGIFISGLS
                     SYFAKDIRKSLAYSTIENSNFMILALVGASIWGMELKNKEIGDSFLFLFYFGLFHHSI
                     SKTFQFLYAGAMTEKAGSTKIDDLKGIGRTIGISPILSGIGAFSFALVPGTTGFFLES
                     SFLYLNSKIFDTSSQNASVYLLVLFLSLTGLAMGSAFHLKFYLSTALSTRKADSFLPT
                     SESESASLRKVFWYLGILIFSVPVFAGPVAVTLYRLNLMKNWLYTLSAVSLIVMIVSF
                     LIYKFHLYHTVSKRQIWDCGSNHSGTEVSIPGSVFSDPVYFSVGRYFTS"
BASE COUNT          509 a          454 c          416 g          729 t
ORIGIN      
        1 gccagaccga taaactttcc ggacacagtc tcaagtgcat ttctgatttt ttttactgtt
       61 tttccgaaga tcattctttt tccctgctga aatacaaaat tcctatgcct gaaaagtcaa
      121 acgtaaaaac tgtcattctc tacctatcaa tttattcttt tcttttttta ttgattctga
      181 aatgctgtct gtatgttcgc aatgagaaaa cggagattct ttatttctgg aaagaaaatc
      241 aagtttcttg catttctctg tctcttctca tttttaagtt ttacagacaa tggactcaag
      301 agcatcgggg ggtcttcttc ggcgcttaaa cctatgatcc gtctttcgtc tgttccagtc
      361 cacagacagt cctcggaatt actggagaac acatctgtaa cttccgagtc gtttctttcc
      421 gaagaaaccg aagacgattt cgaaattatt gctttttttc tttcagagaa tatttcttat
      481 tccattcttc cgtcctccat gcggtatgca aggggcggga tggaatatca gaaaatatct
      541 cttcattata cttataccga tcttccgcct cctgcaaaag tctgattttc aagactaaag
      601 gaggataaaa ttgctaaaat ttctgaaatt tattatattc gcagaactgt tctgttctgc
      661 aggaacggct ctgtatctta tgacccttcc gaatccggat gctgtttcca tcattgggct
      721 tggccttctg tccatcctgg gtctgcctat ttatttttca tccgggatat ttgaggacaa
      781 gggcagacct tcgaggcata tcggcttcat actttttttt gcaggccagt tcgtgtgtct
      841 tatctcaggg aaatccatct ggcttccagt attttgggag ctttcttcaa tcggatgtct
      901 tttcttttac ccagatgaga atcttctgaa aaaatcatcg aagagtgctt ctgcctttct
      961 gctctcaagc ggtctgtcga tgttttttat ctctctctgg atttttctgc ctgattccaa
     1021 ttttggcaga accagtctgc tgataggact tctcataaag gctgcattca gcggttttca
     1081 tgtctggtat ccgattgctc atgaaggtgc tccatattat gcatccgcaa gcatatcagg
     1141 gataatgatg aatctgccta tgctgatgat tgtcaagttt ctatatgcag acctgcatac
     1201 aatgaatgtc actactctga tcatgattct gtgcggtctg ggaattttta tttccggcct
     1261 aagttcttat tttgcaaagg atataaggaa atctcttgca tacagtacaa ttgaaaattc
     1321 caactttatg atacttgcac tggtcggtgc ttccatctgg ggaatggaac tgaaaaacaa
     1381 ggagataggg gacagttttc tttttctctt ttatttcggt ctttttcatc attccatttc
     1441 caagacattt cagttcctgt atgcaggagc aatgactgag aaggcaggat cgacgaaaat
     1501 cgatgatctg aaaggaatcg gcagaacgat aggaatcagt cccattcttt ccggaattgg
     1561 agcattcagc tttgcccttg tgcctggaac gacaggtttt tttcttgagt caagtttcct
     1621 ttatctcaat tcaaagatat tcgataccag cagccagaat gcgagtgtct atctcctggt
     1681 tctttttctg agtcttaccg gccttgcaat gggaagcgca tttcatctca agttttacct
     1741 gtccactgct ctttccacaa ggaaggctga cagctttctc cctacttctg aatcagagtc
     1801 tgcttccctg agaaaggttt tctggtacct gggaatcctt atcttttcag ttcctgtctt
     1861 tgccggacct gtagcagtga ctctttacag actgaatctg atgaagaact ggctctacac
     1921 tctgtcagct gtcagtctga ttgtaatgat agtatcattt ttgatatata aatttcatct
     1981 ttatcatact gtcagcaaaa gacagatctg ggactgcgga agcaatcatt caggaacgga
     2041 agtgtccatt cccggttcag ttttttcgga ccctgtgtat ttttcggtag gcaggtattt
     2101 cacttcgg
//
LOCUS       DAOQGJ010000614         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35551_length_2107_cov_3.055068, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000614 DAOQGJ010000000
VERSION     DAOQGJ010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35551_length_2107_cov_3.055068"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PK683_10170"
     CDS             complement(<1..457)
                     /locus_tag="PK683_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH08857.1"
                     /translation="MIHFPGFETKEILYSGRHSLVYRVLKENRSYVLKYLNYDYPSAE
                     NFRSFVREYETLCQVRMPGVAEAVSLEKYRNSLYILFNDIGGSSVSSYLDREKRIPVR
                     DFFELSEKILRVLNRLHSANLVHRDIKPHNLNYSVKTGELEFLDFGSASF"
     gene            977..1993
                     /locus_tag="PK683_10175"
     CDS             977..1993
                     /locus_tag="PK683_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNH08858.1"
                     /translation="MRISSVISVQKLGFQWGTKNPFLFCVHHEDHFPEGNSDLGPDKS
                     EFKGRNIGQDFEPRDGWRMYHGEKVPGFPHHPHRGFETVTVVQKGYVDHSDSLGASGR
                     YGMGDTQWMTAGKGVQHSEMFPLLNREKDNAMELFQIWLNLPAKKKFSEPFFKMLWAE
                     KIPKYSHSDSNGRKTRIELIAGELADREPISPPPDSWAADPENFVAIWIIEMEPEAIF
                     EIPAIEAGISRTVYFFNGKNIYINDTEIPFYHCADLVSDMDAEIRNGKEKSRLLLLQG
                     RPINEPVVQYGPFVMNSRQEIQEAYDDYVRTEFGGWPWNSGEPVHGNEGRFAKYPDGK
                     KEVP"
BASE COUNT          637 a          451 c          453 g          566 t
ORIGIN      
        1 gaaaacttgc gctgccgaaa tccagaaatt ccagttctcc tgtcttcacg ctgtaattca
       61 gattatgagg cttgatgtcc cgatgaacaa ggttcgcaga atgcagtctg ttcagcactc
      121 tcagtatctt ctcactcagc tcaaaaaaat ctctcacagg aattcttttt tcccggtcca
      181 gatacgagga taccgaactc ccccctatat cattaaacag tatatacagg ctgttccgat
      241 atttttccag tgaaaccgct tctgctaccc cgggcatcct gacctggcac agcgtttcat
      301 actcccggac aaaacttctg aagttttctg ccgacgggta gtcatagttc aggtatttca
      361 gaacataact tcggttctct ttcaatactc tgtatacaag tgaatgtctt cccgaataca
      421 gaatctcttt tgtctcaaat cctggaaaat gaatcaactg aaattccctc cttcaggaat
      481 attctctgct cacgctaaaa tacagactct gatactaaca cttattacag tcttcagtag
      541 ggcagtattt gcgaagcgga aagaatttga cagtatataa aatttaaaat taagtatttt
      601 tgcgtaagaa ctctaattac agtaatgaat tatccggatt gctcagagca aaagaactgc
      661 aggcgtaaaa gctgctgtcc ttgaagtgct aaaactcctt ataaaagcag gcagtggaac
      721 ctgcaaagca aatcagtacc aggtttgcag cagatgccaa atctgcgttc atttaaaatg
      781 gccgaagcag caaagttggc tcagggatta aagtactgcc gccaatgacc ggcttcctat
      841 atgtatattt tcattataaa tccgtttatc ctctctgagt tccggtactt ttcagtcagt
      901 ctgcaggaga gacgggtatt ttattacttg tatctgtaca gcagaggaat attactgcag
      961 tgaggtcggc attattatga gaatcagcag tgttatttcc gtccagaaac tcggatttca
     1021 gtggggaaca aagaaccctt ttcttttctg tgttcatcat gaagaccatt ttccagaggg
     1081 aaactcagat ctgggtccag acaaatcaga gtttaaaggc agaaacatag gacaggattt
     1141 tgaaccgagg gacgggtgga gaatgtatca tggagaaaaa gttcccggtt ttccgcacca
     1201 tccgcacaga gggtttgaaa ctgtcacagt tgttcagaaa ggttatgtgg atcattctga
     1261 ttcactggga gcttccggaa gatacggaat gggggacaca cagtggatga ctgcaggaaa
     1321 aggcgtacag cactcggaaa tgtttcctct cctgaacaga gaaaaagaca atgcaatgga
     1381 gctttttcag atctggctga accttcccgc aaaaaagaaa ttttcagagc ctttctttaa
     1441 aatgctttgg gcagaaaaaa ttccaaagta cagtcactca gacagcaacg gaagaaaaac
     1501 acgaattgag ctgattgcag gcgaacttgc cgaccgagaa ccgatttctc ctcctcctga
     1561 ctcctgggca gcggatcctg aaaactttgt agcaatctgg atcattgaaa tggaaccgga
     1621 agctatattt gaaatccctg cgattgaagc aggaatcagc agaaccgttt acttttttaa
     1681 tggaaaaaat atatacatta atgatacaga aattccattt tatcactgtg ccgatctggt
     1741 atcggatatg gacgcagaaa tcagaaacgg aaaggaaaag tctagactgc ttcttcttca
     1801 gggcagaccg atcaatgaac cggtcgtaca gtacggtccg tttgtcatga attccagaca
     1861 ggaaatccag gaagcatacg atgactatgt cagaaccgaa ttcgggggct ggccatggaa
     1921 ctccggtgaa ccggttcacg ggaatgaagg acgattcgca aaataccctg acgggaaaaa
     1981 agaagttcct tagaatcagg taaaagcttg ctaatttcat tcttttgaaa tattcaggaa
     2041 gaatgaaatt ttccgtatca gtccttctcc ttgtcggtct gcatctgcct gtattcgcag
     2101 aaagaac
//
LOCUS       DAOQGJ010000615         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35644_length_2103_cov_3.867676, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000615 DAOQGJ010000000
VERSION     DAOQGJ010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35644_length_2103_cov_3.867676"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PK683_10180"
     CDS             complement(<1..1083)
                     /locus_tag="PK683_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08859.1"
                     /translation="MKINGKNIFLHSLLICSLLFSDCRKTENKSKDNLLKAGAVLVLA
                     NSVSSGTTGLTIRIPEGVPLKTNVASNTKNRSLWKFLKAQMNFPEAKASENQWAFVRQ
                     SALWANNNTDAIEAVLNPIKKYNLLNSPGTLTTTVQLASGKLYTAKLTVSANTVVSSS
                     AYTGTKTFNHRFEMWRQSDNAKAMEMFFDSADALSEKGILLLYNLNILDPSSYPDSLV
                     CETYVSSQTVSYLGSTVSSPKQTISWSGTQSFYTGSLGGRVVLEKMDNNTVLCFKSVV
                     RFQHSATFSGCGTGTKYYALAYSQYLNTSDNKATAKVSLADSSIDNGQTSKICSAYSL
                     NYGLFINKSGFVSDTNAASAVPSDYPS"
     gene            complement(1099..1557)
                     /locus_tag="PK683_10185"
     CDS             complement(1099..1557)
                     /locus_tag="PK683_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08860.1"
                     /translation="MKKELFQTAVFLLISFLSAHCSSEKVSVKMSMDGKVLFETKGAD
                     VGKLSPENGGYRFGSIYVIYDTTEAKDGTVKDISYRNGSEYITMRPAVYSSARGSINC
                     KSAKDLNRTTGEPPFPTDIFGRVKHGTYYFEMAEPCGRLEFTVSASSKTE"
     gene            complement(1644..>2103)
                     /locus_tag="PK683_10190"
     CDS             complement(1644..>2103)
                     /locus_tag="PK683_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08861.1"
                     /translation="IGSIVMFFSFSKREILFVRKKKSSLVGIKLKRVLKSVPSCWALL
                     LLSSSVLNFSFLYSVLNSEENLSDYTINSFIISAASVFQESSSEYSIFFPRLSLPNEN
                     LSSGNRINFSGRNFRFADFRGSVLNLNSFHLAHTEHADFAESRWVSKVGE"
BASE COUNT          555 a          437 c          455 g          656 t
ORIGIN      
        1 agagggataa tcggaaggta ctgccgatgc tgcattcgtg tctgatacaa atccgctttt
       61 gttgatgaaa agtccgtaat tcaggctgta ggcactgcag attttggatg tctgtccatt
      121 gtcaatggaa ctgtctgcaa gacttacttt tgcagtggcc ttattgtcgg acgtattcag
      181 atactggctg tatgcaagtg catagtattt tgttccggtt ccgcatcctg aaaatgtcgc
      241 actgtgctgg aaacgcacaa cagacttgaa gcagagcaca gtattattgt ccattttctc
      301 cagtacaact cttccgccga gcgagcctgt gtaaaaggac tgtgttcccg accaggatat
      361 agtctgcttc ggagaactga cagtggaacc taggtaggaa actgtctgtg aggatacata
      421 ggtttcacat acaagactgt ccggataact tgacggatcc agaatattca ggttgtaaag
      481 aagaagaatt cccttttctg aaagcgcatc tgcactgtcg aagaacattt ccattgcttt
      541 tgcattgtcg gactgtctcc acatctcaaa cctgtgattg aatgtcttag tgcccgtata
      601 tgcagaagag gaaacaactg tatttgcact tactgtaagt tttgcagtat aaagcttgcc
      661 gctcgcgagc tgaactgtcg ttgtaagcgt tcccggactg ttcagaagat tgtatttttt
      721 gatcgggttc agaactgctt caattgcatc tgtattgttg ttcgcccaga gtgcggactg
      781 ccttacaaaa gcccactgat tctccgaagc ctttgcttca ggaaaattca tctgcgcctt
      841 taagaatttc caaagagatc tgttctttgt attggatgcc acgtttgtct ttaaaggcac
      901 accttccgga attcgaatcg tcagtcctgt agtgccggat gacacactgt ttgcgagaac
      961 aaggacagca cctgctttca gaagattgtc cttgctctta ttttctgttt tcctgcagtc
     1021 tgaaaaaagt aatgaacaga tcagcagaga gtgcaggaat atattttttc catttatttt
     1081 cataattgag tttccttttt attcagtttt tgatgaggct gataccgtaa attccagccg
     1141 gccgcagggt tctgccattt caaagtagta cgttccgtgt ttgactcttc caaaaatatc
     1201 tgtcggaaaa ggaggctctc cggttgtcct gttcaggtcc tttgctgatt tgcagtttat
     1261 gctgcctctg gctgaactgt agacagcagg tctcatcgtg atatattcag agccgtttct
     1321 gtagctgata tccttgacag ttccatcctt tgcttcagtt gtatcataga taacatatat
     1381 ggatccgaaa cggtatcctc cgttttctgg actgagtttt cctacatctg ctcctttcgt
     1441 ctcaaacaga actttcccgt ccattgacat ttttacagac actttttcac tgctgcaatg
     1501 agcggacaga aatgagatca gaaggaatac agctgtttga aagagttctt ttttcattat
     1561 ttttattctc ctggaaaatg ggggatctga atctcatttg tatcttcagt aaggtgcaga
     1621 gtcaacattt ttttgtacag aaatcattct cccacttttg acacccatct gctctctgca
     1681 aaatctgcgt gttcagtatg agccagatga aatgaattca gattgaggac ggaacctctg
     1741 aagtctgcaa atcggaaatt tcttcctgag aaattgatcc tgtttcctga tgacaggttt
     1801 tcatttggaa gactcagcct tggaaaaaaa atagaatatt ccgaactgct ttcttgaaag
     1861 accgaagcgg cggatatgat aaaagaattt attgtataat ccgagagatt ttcctctgag
     1921 ttcagaaccg aatacaggaa ggagaaattc agaacagacg atgagagaag aaggagtgcc
     1981 cagcaggaag gcacggattt cagaactctt ttcagtttta taccgacgag actgcttttc
     2041 ttttttctta caaacaggat ctctcttttg ctgaatgaaa aaaacattac aatggaaccg
     2101 atc
//
LOCUS       DAOQGJ010000616         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35670_length_2102_cov_4.250122, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000616 DAOQGJ010000000
VERSION     DAOQGJ010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35670_length_2102_cov_4.250122"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /locus_tag="PK683_10195"
     CDS             complement(<1..1964)
                     /locus_tag="PK683_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08862.1"
                     /translation="MEKSENALFKFSHDRIQQAAYSTIPEESRPTVHLQIGRNLLKKY
                     SDREDRLFLITDHLNLGSSLIENETEIMRLIELNLLSAKKSKEAAAYDSALQYLKNND
                     PLLEKKDLRQNIWKKYYTTAYKYYILKGETEYLSGQLEESEKSISLAEKNAADILDRA
                     NAYQILIVQKTLLAEYENAISTGRTALSLFGIRLPDSDYEKFRDALLAEIDELTEGRT
                     VGSLFDLPDMTDPEKRMAVNLLITMGPPCYRSHPRLWAVIVSTAVKLSIQYGNFPEAG
                     YSHTAFGGMIGFVRGEYRRGKEFGELAERLMTEKFHSPAHRSVFFLMIGSSLRHWSFP
                     LSQSSRDYQSAYDAGVESGNLQYAAYAFGHNMYCLFFQGMKLPDLRTEIEKYLKFSRI
                     RKNWWAIDLLEGGLIIINYLMHGGDFSLDSMSENEYLQRCESHRNIQVVCIYNVMKTF
                     ALFILEKYSDAEKSFLEAEKRILSAAVQGLLPYSEHRFIQCLLILHRYRSASEEERKE
                     YDRILDQNAELLKIWSENCPENYVFKYFLVLAEISRQKNDILTALNYYDKAVQFSEES
                     GASIFQAVALELSAKFWNRQGRVRLSKPYMTEALYVYRMWGAEFKTSELRRKYEKSDR
                     VTEREYSTYRFSDVFTEFRSAEIDTGSLLK"
BASE COUNT          533 a          468 c          447 g          654 t
ORIGIN      
        1 attttaagga gactgcctgt gtctatttca gcagatctga attcagtgaa gacatcggaa
       61 aacctgtagg tggagtattc tctttcagtg accctgtcgg atttttcata ttttctccgc
      121 agttcagagg tcttaaattc cgctccccac attctgtaga cataaagcgc ttcggtcata
      181 tacggcttgg acagtctgac tcttccctgc cggttccaga actttgcaga cagctccagc
      241 gcgaccgcct gaaatatgga agctccgctt tcttcactga actgtacagc cttgtcataa
      301 taattcagtg ctgtaagtat atcgtttttc tgtctgctga tttcggccag tacgagaaaa
      361 tatttgaaaa catagttttc aggacagttc tccgaccaga ttttcaggag ttctgcattc
      421 tgatccagaa tcctgtcata ctcctttctt tcttcttccg aagcagatct gtatctgtga
      481 aggatgagaa ggcactgaat gaatctatgt tctgaataag gcagaagtcc ctgcactgca
      541 gcggacagta ttcttttttc cgcttccagg aatgattttt ccgcatcact gtatttttca
      601 aggatgaaaa gtgcaaatgt tttcattaca ttgtaaatac acacaacctg gatgtttctg
      661 tgagattcgc atctctgcag atattcattt tcggacattg agtccagact gaaatctcct
      721 ccgtgcatta gataatttat aatgatcaga cctccttcca gaagatcgat tgcccaccag
      781 ttttttcgga ttctgctgaa tttcagatac ttttcaattt cggttctgag atcaggaagt
      841 ttcattcctt gaaagaaaag gcaatacata ttgtgtccga aagcataggc ggcgtactgc
      901 agattgcctg actcgactcc tgcatcatag gcactctggt aatcccttga ggactgggaa
      961 agaggaaatg accagtgccg aagtgaactt cctatcatca gaaagaatac gcttctgtgt
     1021 gccggagaat gaaatttttc cgtcatcagt ctttctgcca gctctccaaa ttcttttcct
     1081 cttctgtatt cacctcttac aaatccgatc attcctccga atgcagtatg gctgtatcct
     1141 gcttcaggga aattgccgta ctgtatgctc agtttgactg ctgtagaaac aatgactgcc
     1201 cagagtctgg gatgagatct gtagcaggga ggtcccattg tgatgagaag attcactgcc
     1261 attctttttt ccggatcagt catgtccggc agatcaaaaa gagaacctac tgttcgccct
     1321 tcggtcagtt catcgatttc agccagcagg gcgtccctga atttttcata gtctgaatca
     1381 ggaaggcgaa tgccgaacag actcagtgca gtccttcccg tactgattgc attttcgtat
     1441 tctgcaagga gagttttctg aacaatcagt atctgatatg catttgcacg gtccagaata
     1501 tctgcagcat ttttctctgc gagcgatatg cttttttcag actcttccag ctgtcctgaa
     1561 agatattctg tctctccctt cagaatataa tatttatatg cggtagtata atattttttc
     1621 catatattct gcctgagatc ttttttttcc aggagaggat cattgttctt cagatactga
     1681 agagctgaat cgtaggctgc cgcctccttg gattttttgg cagaaagaag gttaagttca
     1741 atcagacgca ttatctctgt ctcattctca atcagcgagg aaccgagatt cagatgatct
     1801 gtaatcagga aaagtctgtc ttctctgtca gagtatttct tcagaaggtt tctgccgatt
     1861 tgaagatgta cagtgggacg agactcttcg gggattgttg aatatgctgc ctgctgaatt
     1921 ctgtcatgag aaaacttgaa gagtgcattt tctgactttt ccacactgag gtccagggga
     1981 aaaatgagcc cctccttggc tgccggcaca agtcttccgg aacattcgga gatatcgagg
     2041 tttacagata cggagaatgt ttccagatca aacatattcc cgagacagga cgctgaagca
     2101 ag
//
LOCUS       DAOQGJ010000617         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35677_length_2102_cov_3.274060, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000617 DAOQGJ010000000
VERSION     DAOQGJ010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35677_length_2102_cov_3.274060"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PK683_10200"
     CDS             complement(<1..985)
                     /locus_tag="PK683_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNH08863.1"
                     /translation="MKFIIFSLLWTVSVFSESLKVGIAGSAPFRISEPKVSGISLDIW
                     DKMAEMEKWDYTVKEYPNISALIEAVNRGEMDVGIGPITVTSKRLESVSFSQPYYSSH
                     KAILVKRGSISPMSFVRTLFEETFFYAIGILFCVLFLVGNLILLAEKGVNPAFQLPYF
                     SSIGNAMWLAVVTFSTVGYGDITPKTKTGKLIIASWMVISMITASSLTAGIAASFTLS
                     RIQNHAIDSPEDMKERRIAAILGSTGEEIVKAYKGYLVSIENAKEGIQSVRDNKADGF
                     IGDYPIIKYNLENNAFPTAAIVEFKTDKDSYGFCMSKGNRKLERVNHTLLIL"
     gene            complement(982..>2102)
                     /locus_tag="PK683_10205"
     CDS             complement(982..>2102)
                     /locus_tag="PK683_10205"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH08864.1"
                     /translation="KKKYVAFLNADAMGKSIQGAGGALVLGAVFHSIIQRTHTYKVQS
                     EVAPEYWIKNTFKEMHKIFESFNGSMLISLVFGLVEENTGLVYFINAEHPWVIEYKNR
                     QASFIEHNLSFRKLGTSGVESEIFISTYQMTPGSLLIMGSDGKDDVVLKGRPAEDRSV
                     NEDETLFLKKVEEADADLNRIFSLISDQYELIDDFSLLSITYNGDESYYTQDELQEQE
                     IIMKRGAAYFRQARYEDAIAILKYGHDAYRLNSHIAGFLVRTYMKAGKHKEAAEAGLD
                     FLLIKDSDTNLMLRVSYALKKNRRIEEAIDLSERIKLREPSNIPNLVHLADMYTYRRN
                     FRRADKLLNKIFRLEPENEDAIKIRLKLDSFASEAAVS"
BASE COUNT          554 a          512 c          411 g          625 t
ORIGIN      
        1 ccagaatcag aagggtgtga ttgactcttt ccagttttct gtttcctttt gacatgcaga
       61 atccgtatga atccttgtct gtcttgaatt ccacaattgc tgcagttgga aatgcattgt
      121 tttccagatt gtatttaatg atcggataat ccccgataaa accgtctgcc ttattgtccc
      181 gcacagactg aattccttcc tttgcatttt caatggatac aagatagcct ttgtatgcct
      241 tgacaatctc ctctccggtg ctccctagaa tcgctgcaat ccttctttct ttcatatctt
      301 cgggagaatc aattgcatga ttctgtatcc tcgaaagagt aaaggatgcc gcaattcccg
      361 ctgtaagaga tgaagcagta atcatgctga tcaccatcca ggaggcaatg atgagttttc
      421 cagtctttgt cttcggagtg atgtccccgt agcccactgt gctgaatgtg acaaccgcaa
      481 gccacatcgc atttccgata ctgctgaaat agggaagctg aaaagccgga ttcactccct
      541 tctctgcaag aagaatcaga tttcccacca gaaaaagaac acagaaaaga atcccgatcg
      601 cataaaagaa agtttcctca aaaagagttc tgacaaaact cataggagaa atggagcctc
      661 tttttacaag gattgcctta tgactggaat agtacggctg tgaaaaagaa actgactcca
      721 gtcttttgga tgtcacagta ataggaccga ttccaacatc catttccccc ctgttcacgg
      781 cctcgatcaa tgcacttata ttagggtatt ccttgactgt ataatcccat ttttccatct
      841 ccgccatctt gtcccagata tccaggctga ttccggaaac cttaggctca gagattcgga
      901 aaggtgctga acctgctatt ccgaccttga gggattcaga aaaaactgag acagtccaaa
      961 gtaatgaaaa aataatgaat ttcatgatac agctgcctcg gacgcaaaag agtcgagctt
     1021 cagtcttatc tttattgcat cttcgttctc aggctccagt ctgaatattt tattcaggag
     1081 cttgtctgcc cgtctgaaat ttcttctgta tgtgtacatg tccgcgagat gcaccaggtt
     1141 cggaatattg gaaggctccc tcagcttgat ccgttctgaa agatctattg cttcttctat
     1201 gcgtctgttt tttttcagtg cgtaagatac cctgagcatc agattcgtgt cggaatcctt
     1261 gatcagaaga aaatccagac ctgcctccgc agcttctttg tgctttcctg ccttcatata
     1321 tgtccttaca agaaaacctg cgatatgaga attcagcctg tatgcgtcat gtccatactt
     1381 cagaattgca atcgcatcct catatctggc ctgcctgaaa taggcagctc cgcgcttcat
     1441 tataatttcc tgctcctgaa gttcatcctg agtgtaataa ctttcatccc cgttgtacgt
     1501 aatggaaagt agagaaaaat catcgatcag ttcatactga tccgaaatca ggctgaatat
     1561 cctgttcaga tcagcgtccg cctcctccac ttttttcaga aaaagagttt catcctcgtt
     1621 gactgatcgg tcttccgcag gcctgccctt cagcacaacg tcgtcctttc cgtccgatcc
     1681 cataatcagc agagaacccg gagtcatctg ataggtggaa ataaatattt cactctccac
     1741 tcccgacgtg ccgagttttc tgaagctgag attatgttca atgaaactgg cctgtctgtt
     1801 cttatattct atgacccagg gatgttctgc attgatgaaa tataccagtc cagtattttc
     1861 ctccaccaga ccgaatacga gagagatgag catggaaccg ttgaaacttt caaatatctt
     1921 gtgcatttcc ttgaatgtat tcttgatcca gtattcaggt gcaacctcag actgcacttt
     1981 gtacgtatga gtcctctgta tgatggaatg aaagactgcc ccaagaacaa gtgcaccacc
     2041 tgctccctga atggattttc ccattgcatc tgcattcaga aaggcaacgt acttcttttt
     2101 ct
//
LOCUS       DAOQGJ010000618         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35734_length_2100_cov_2.854279, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000618 DAOQGJ010000000
VERSION     DAOQGJ010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35734_length_2100_cov_2.854279"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PK683_10210"
     CDS             complement(<1..465)
                     /locus_tag="PK683_10210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; subunit A is part of the
                     membrane proton channel F0; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0F1 subunit A"
                     /protein_id="HNH08865.1"
                     /translation="MNKLAALILSITLSTAAMPLLASDSSHSEKFDLADVIDHHLADH
                     AEFPLNFGGEKVFEGDPKFDPSHHDVFVDHHANKKYHFVGGLDMHITKRVTMMWIVAF
                     FLIVILVPLARKIASNPYKVQSTGANMLETILIYLRDEVIEPNMHGHGHGYYG"
     gene            complement(458..907)
                     /locus_tag="PK683_10215"
     CDS             complement(458..907)
                     /locus_tag="PK683_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08866.1"
                     /translation="MQKHSDFQRLSSVSSGKNDRALDLSQVPFWQFVFFYILLTAVWM
                     TGAFILVRKMNWHQSYLTGIYAGSLIQGGFSVFKVFLMNREWPNPPMAQMFFMLLSFI
                     ISLIVLSYFSIFTGDNAFLVGFFIAYYLNVKIIVLIAYFYSGKEKDE"
     gene            complement(912..1895)
                     /locus_tag="PK683_10220"
     CDS             complement(912..1895)
                     /locus_tag="PK683_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08867.1"
                     /translation="MKILIISVLLFSGCSHYTRAAGGQDTSYASAFPVLKKKKIVLLG
                     MVPFKTERGSAVNPYSGDPDFLKMLLFIDKAYEQGSSESRFSFSNYRSSGRYGGGGYQ
                     SWKREPDKANSLIKEGDYGRTLKDVKFGAPDANVREKDFAAFTGLYYQYYGFHAFADL
                     KKIAVFPKDEKQKNQLNFTLKAKSSDADYWVIVLPSVFPGFHPADPLNPLSALLSKAL
                     MAVLSIYSLGLIPFSQEYNKTFYYTVFDSRYRKIRSGYLSSRKETYLSVRKWNEDDIR
                     LDGPNSPVLFETENRRLAEELAELLENGGARTEKSLKGTLPAGRGRRISEK"
BASE COUNT          610 a          478 c          434 g          578 t
ORIGIN      
        1 cccgtaatat ccatgaccgt gaccgtgcat attcggctca atcacttcat ctctgagata
       61 tatcagtatc gtctcgagca tattcgcacc cgttgactgc actttgtaag ggttgcttgc
      121 gatttttctg gcaagcggaa caaggatgac gatcaggaaa aatgctacga tccacatcat
      181 tgtgaccctt tttgtaatgt gcatatcgag cccgcctaca aaatggtact ttttgtttgc
      241 gtggtggtct acgaatacat cgtggtgtga aggatcaaac ttaggatcac cttcaaacac
      301 tttttctccg ccgaaattca gaggaaactc tgcatggtct gcgaggtgat gatcgatcac
      361 atcggcaaga tcaaattttt cggaatggga tgagtcactt gccagtaaag gcattgctgc
      421 agtgctcagt gtgatggaca ggattaaggc ggctagttta ttcatctttc tctttgccag
      481 aataaaaata agcgattaat acaatgattt ttacattgag ataatacgca ataaaaaacc
      541 cgactaaaaa cgcattgtct ccggtaaaaa tcgaaaaata ggagagaaca atgagactga
      601 ttataaaaga caataacatg aaaaacatct gtgccatcgg cggattcggc cattcgcggt
      661 tcattagaaa aaccttaaat accgagaatc ctccctgaat cagcgatcct gcatagatgc
      721 cggtgagata actctgatgc cagttcatct ttcgaaccag gataaatgcg ccggtcatcc
      781 agactgcagt cagaagaata taaaaaaata caaactgcca gaatggaacc tggctcagat
      841 caagcgccct gtcgttttta ccggaactca ctgaactcag tctttgaaaa tcagagtgtt
      901 tctgcaagtc attatttttc cgaaatcctc cttcccctgc cggcaggaag agttcctttg
      961 agagattttt cggttctggc tccgccattt tccagaagtt cagcaagttc ttccgccaga
     1021 cgcctgtttt cggtttcgaa gagaacaggc gaattcggtc catccagcct gatatcatct
     1081 tcattccatt ttcggacaga caaatatgtt tccttcctgg aagaaaggta tcctgatctg
     1141 attttcctgt atcggctgtc aaaaactgta taataaaatg ttttattata ttcctgactg
     1201 aagggaatca gtcccaggga ataaatgctc aggacggcca tcagagcctt gctgagcaag
     1261 gcactcaacg gattcaaagg atctgccggg tgaaagcccg ggaaaacgga aggcaggaca
     1321 atcacccagt agtctgcatc ggaggacttg gccttcagag tgaaattcag ctgatttttc
     1381 tgcttttcgt ccttaggaaa aacagcaatt ttcttcagat cagcaaatgc atggaaccca
     1441 tagtactgat aatacagtcc cgtaaaggca gcaaaatcct tctctctgac atttgcatcc
     1501 ggtgctccga atttcacatc tttcaaagtg cggccatagt ctccctcctt tatcagggaa
     1561 ttggctttgt ccggctccct tttccagctt tgataacctc cgcctccgta tcttccggaa
     1621 gaacggtaat tactgaagct gaatctgctt tctgaacttc cctgttcata tgccttgtcg
     1681 ataaacagga gcattttcag aaaatcagga tctccggaat atggattcac tgcactgccc
     1741 cgttctgtct taaaaggcac cattcccaaa aggacgattt tctttttttt cagaacaggg
     1801 aaagcggaag cataggatgt gtcctgacct cctgccgccc ttgtataatg cgagcatccc
     1861 gaaaaaagca gaactgaaat aatcagtatt ttcataagaa cagtttcgat atattcggaa
     1921 tatatgcaat gggtatgttt ccctctcagg gaaactgccc aaggcaatag aggaaattat
     1981 ctatccgaaa tgaattggaa catacacaaa cacaaaatgg aataaaacgg accagaccaa
     2041 atttctaatg aaattttcat gcagtcgatt attcatttaa atttcgtcct gcaggcctgc
//
LOCUS       DAOQGJ010000619         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35783_length_2098_cov_3.637788, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000619 DAOQGJ010000000
VERSION     DAOQGJ010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35783_length_2098_cov_3.637788"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2071
                     /locus_tag="PK683_10225"
     CDS             <1..2071
                     /locus_tag="PK683_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH08868.1"
                     /translation="ETKKLLSAGTDQIFAAILDLELPDALQGEIVDFVSAYRLPSIVF
                     TGKNDETSSLFFNSKPVLDFISKEGASNFHFVARILENLKKNSSENILLISADTGKDS
                     DISDLLRLHNFQITKICSVEDVSSALEKNPGIRLIVSDFIKPTSGDFDFIEKFREKKD
                     KSDFGIIAVISDRDHLSISKLLKRGVNDCIYTPILKEEFFSRIYNLIDNLDNYKLLKK
                     SQYELRKEEKHLREIMDGVDESSIVSRSDIYGKITYVNRQFCEISGFSREELLGKPHN
                     IIRHPDMPASAFADLWKTIKAKKIWTGIIKNRKKNGDAYYVKTLIKPVLDLNGDIEEY
                     ISIRQDITDQEEARQGLLQKLSVRSSSINEIQHQSKLFEDILNESSLLMNVGLDGKIK
                     SVNEQYSRVTGFLLSELKSLTVEDIQIKSQEDILTVEKLLSMGSEDRWKGTFCIKDKT
                     ERTLYLNAYVLPLKNTVGKMEEYLITLSDITRVMQYHYSMEKAQKELIYIITEAIESN
                     SQETGFHVQRVGEYAGLLGSIYGLSKADCETLRITAALHDLGKIGIPDSILNKPGRLD
                     ENEWKIIKTHSEIGYKILQHSNLDILKKASMIAKEHHERWDGTGYPQGLKGGEINIFA
                     RITSVADVFDSLNSERCYKNAWDRERILNYFLEQRGHQFDPILVDLLLDNAEKFDAVK
                     KKIRELS"
BASE COUNT          716 a          405 c          450 g          527 t
ORIGIN      
        1 agaaacaaaa aagctgcttt ctgccggaac ggatcagatt ttcgccgcga ttctggatct
       61 tgaacttccg gatgcccttc aaggcgaaat cgtagatttt gtctctgcat acaggcttcc
      121 ttccattgta tttaccggca agaacgacga aacatcctct ctgtttttca acagcaagcc
      181 tgtactggat tttatttcaa aggaaggtgc atccaatttc cattttgtag caagaattct
      241 ggaaaatctt aaaaagaaca gttcagaaaa tattcttctg atcagtgccg acactggaaa
      301 agattccgat atttcggatc ttctgcgtct gcacaatttt cagattacaa aaatctgctc
      361 ggttgaagat gtttcctctg ccctcgaaaa aaatcccggc atccgtctga ttgtttccga
      421 tttcataaag ccgacatccg gcgattttga ctttattgaa aaattcagag aaaaaaagga
      481 caagtcagac ttcggaatca tagcagtcat atctgacaga gatcatctca gcatatccaa
      541 gcttctgaaa agaggagtaa acgactgcat atacactccg attcttaaag aggaattttt
      601 ttccagaatt tataacttaa ttgataattt agataattat aaacttctca agaaaagtca
      661 gtatgaactt cgaaaagagg aaaaacatct tcgggaaatt atggacggtg tcgacgaaag
      721 ctccattgtg tccaggtccg acatctacgg aaaaattaca tatgtaaaca ggcagttctg
      781 tgaaatcagc ggcttttcca gagaggagct tctgggaaaa cctcataata taatccgtca
      841 tcctgatatg ccggcatctg catttgccga tttatggaag acaataaaag ccaaaaaaat
      901 atggacaggc attataaaaa acagaaagaa aaacggtgat gcctattatg taaagacact
      961 tattaaacct gtactggatc tgaacggaga catagaggaa tatatcagca tacggcagga
     1021 tatcaccgat caggaggagg ccagacaggg acttcttcaa aagctcagtg tccgcagttc
     1081 cagcataaat gaaattcagc atcagtcaaa gctttttgaa gatatcctga atgaaagcag
     1141 tctgctgatg aacgtcggac ttgacggaaa aataaaatca gtcaatgaac agtacagccg
     1201 cgtgactgga tttctgctca gtgaactgaa atccctgaca gtggaagaca ttcagatcaa
     1261 aagtcaggaa gacattctga ctgtagaaaa gcttctttca atgggcagtg aagacagatg
     1321 gaaaggcaca ttttgcatca aagacaagac ggagagaact ctttatctca atgcatatgt
     1381 acttcccctg aaaaatacag tgggcaagat ggaagagtat ctgatcactc tctctgatat
     1441 cacaagagta atgcagtatc attacagtat ggaaaaagct cagaaagaac tgatatacat
     1501 cattacagaa gcgatagaaa gcaactctca ggaaactggc tttcatgtgc agcgtgtagg
     1561 ggaatatgca ggactgctgg gttccatcta tggtctcagc aaggctgact gtgaaactct
     1621 gcgaattaca gcagcccttc atgatcttgg caagatagga attccggaca gcattctgaa
     1681 caagccggga aggctggatg aaaatgaatg gaaaattatt aaaactcact cagaaatagg
     1741 atataaaata ctgcagcatt ccaatttgga catactgaag aaagcatcta tgattgccaa
     1801 agagcatcat gagcgatggg acggcaccgg atatcctcag ggactgaaag gaggagaaat
     1861 caatattttc gcaagaatca catctgttgc ggatgtattt gattcactga acagtgaaag
     1921 gtgctacaaa aatgcctggg acagagaaag gatactgaat tattttcttg aacagagagg
     1981 acatcagttt gatcctatcc tggttgatct ccttctggac aatgcagaaa aatttgacgc
     2041 agtcaaaaaa aaaatacgag aactgagctg agcggggcct gaatgaatcc ggaaaaaa
//
LOCUS       DAOQGJ010000620         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35824_length_2097_cov_3.557786, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000620 DAOQGJ010000000
VERSION     DAOQGJ010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35824_length_2097_cov_3.557786"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /locus_tag="PK683_10230"
     CDS             complement(<1..1903)
                     /locus_tag="PK683_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08869.1"
                     /translation="MNIGSKIKQYIFFTVNGLILSVLIAFSLEFYSVSIKSTHELAFR
                     VSEVWASRFKNILSDPLGGIRDLRKVFLKKGLLNRTSAIDLLRTHTEEKEKLVGTYIA
                     FEPNAFDGQDAVYVNSAGHDSTGRFVPYLVRNREGKIILEPTTEYDTVDWYNLPKKSL
                     KNHLTEPYYDTIEGKYLFLTSISLPLIREGKFLGTIGVDLTLDFLQMEFGKTKPYRDS
                     GYINLISNGGVYIINGLDEKLKSKSVKEILHGAEILKNLSENGSYIYEDSEYYHVILP
                     VELENTVEKWYVETAFSKNELFFDTFREIIMIIAGAVILSVFGAFFFNRLLNAMIYGP
                     LNKAVRISKQIAEGDLNVKEEITETGEMEILLSSVRSIAVNNSVLVREMKNSNLHLLQ
                     TARFLLDSAKQIQKGSERQTESAVNTNSASVQLSRSSDSIESSMILAKKNLDSIELNI
                     TELNDSIHQISSGMLEFEKLAKSSSLQAKKGTEAVDRAASAMNAIMQTTKEISKFSRI
                     ISEISGRTNLLALNAAIEAARAGEAGKGFSVVADEVAKLAGSTVESVRSISSLMSQAE
                     ESVRNGILQINDTINIFKSIISNVEVLNSSSGEITKELLEQSDNSAKISSNLNSLSHL
                     TEEILRSSTE"
BASE COUNT          609 a          437 c          389 g          662 t
ORIGIN      
        1 gctcagtgct ggaacgaagt atttcttcag tcagatgact gagagaattc agattcgatg
       61 atattttagc agaattgtcc gactgctcaa gaagctcttt ggttatttca ccggaagatg
      121 aattcagcac ctctacattg gatataattg acttaaatat atttatagta tcattaatct
      181 gcagtattcc gtttctcact gattcctccg cctggctcat aagagaagaa attgatctga
      241 ctgattcaac tgtactgcct gcaagtttcg ccacctcatc tgctaccacc gaaaagcctt
      301 ttcctgcctc acccgctctg gcagcttcaa ttgcagcatt cagtgcaagc agatttgttc
      361 tgcccgatat ttcagatatg atcctggaaa actttgagat ctccttggtt gtctgcataa
      421 tggcattcat tgcggatgct gctctgtcca ctgcctctgt gccttttttg gcctgaagag
      481 aagaggactt tgccagcttc tcaaattcga gcatacctga actgatctga tgtattgagt
      541 cattcagctc agtaatgttc agctcaatac tgtccagatt tttctttgca agaatcatgg
      601 aagattctat tgaatcagaa gaacggctga gctggaccga tgctgaattg gtattgactg
      661 cagattcagt ctgtctttct gaacctttct gaatctgctt tgcagagtcc agaaggaacc
      721 ttgcagtctg aagaagatga aggttgctgt ttttcatttc cctgaccaga acggaattgt
      781 tcacagcaat acttctgaca gatgaaagaa gaatctccat ctctccagtt tcagtaatct
      841 cttcctttac attcagatca ccttctgcaa tctgtttgga aatcctgact gccttgttca
      901 aaggaccgta aatcattgca ttcagaagtc tgttgaaaaa aaatgcccca aagactgaaa
      961 gaatcactgc tcctgcaata atcatgataa tttccctgaa tgtatcaaaa aataattcat
     1021 ttttagaaaa tgcagtttca acgtaccatt tctcaactgt gttttccagt tcaaccggaa
     1081 gaattacgtg atagtattcg gagtcttcat agatatatga gccgttttca gaaagatttt
     1141 tcagaatttc ggctccgtgc agaatttctt tcactgattt gcttttaagt ttttcgtcca
     1201 gaccattgat gatatacacc cctccgtttg atatcagatt gatgtaaccc gaatctctgt
     1261 aaggttttgt ttttccaaat tccatctgca gaaaatccag agtcagatcc actccgatag
     1321 tgcctagaaa tttcccttcc ctgatcaagg gaagagaaat cgatgtaagg aaaagatact
     1381 tgccctcaat tgtatcataa tacggttctg ttaaatgatt ttttaatgat ttcttgggga
     1441 gattgtacca gtctacagta tcatattcag ttgtcggttc cagaatgatt tttccttccc
     1501 gatttctgac aagatatgga acaaatcttc cggtagaatc atgacctgca gaattcacat
     1561 aaacggcatc ctgaccgtca aatgcattcg gctcaaatgc aatatacgtc ccgaccagtt
     1621 tctctttttc ttccgtatgc gttcttaaaa gatcaattgc agatgttctg ttcagaagac
     1681 cctttttcag aaaaactttt cgaaggtctc tgattccgcc gagagggtct gacagaatat
     1741 ttttgaagcg ggatgcccag acctctgaaa ccctaaaggc caattcatgg gtggatttga
     1801 tgcttacaga ataaaattca agggaaaacg cgataagtac agaaagtata agtccattta
     1861 cagtaaagaa tatgtactgc ttgatttttg atcctatatt caatcttttc cctatgaaaa
     1921 ataagcttca ggataacaaa acaaaaagtc tatcagcaga ttaagaaatt cgttcaaata
     1981 tccttacaaa aaaacagatt actctgttcc cgtatcgaaa ttaaggcagt tttaattgta
     2041 aaatattcgt aactactcag cgaattttgg agagaatggg ttcccaagga aagcatt
//
LOCUS       DAOQGJ010000621         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35910_length_2094_cov_3.231486, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000621 DAOQGJ010000000
VERSION     DAOQGJ010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35910_length_2094_cov_3.231486"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK683_10235"
     CDS             complement(<1..278)
                     /locus_tag="PK683_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH08870.1"
                     /translation="MIVDSSALLAILFSEPEKEEFLQIIQREQCSMSGANYLESAIRV
                     DSLRDPVLSRIFDELIRELKIKIEPVTVEQILEARNAFKDFGKGSGHKA"
     gene            complement(275..529)
                     /locus_tag="PK683_10240"
     CDS             complement(275..529)
                     /locus_tag="PK683_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNH08871.1"
                     /translation="MALSLKDPETDQLARKVSQLTGETITQAITIALRERLDRLEHNY
                     DMMRKVRIQEILDQFKAKVPLTAKSSDHSEMLYGKDGLPK"
     gene            complement(579..1235)
                     /locus_tag="PK683_10245"
     CDS             complement(579..1235)
                     /locus_tag="PK683_10245"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HNH08872.1"
                     /translation="MKIEDYKAKFTEEDAVGWLSIDSAIEKIYGKTQPRHYGPPCGIH
                     YIAGGTDPIDGVSIYDSKKQTPHVHLVSYGMSELYYNEEACEDQLSQWGFEFTMRLVP
                     FKDDPGDPFWIIQMMNNLARYVFSSGKWFEENHFIPANGPIRLNTETEITGLVFALDP
                     ELGKIDTPHGEVQFLQMVGITSGELERLKKNPSLDEVEKLISELKKENPLLITDLNRK
                     "
     gene            complement(1600..>2094)
                     /locus_tag="PK683_10250"
     CDS             complement(1600..>2094)
                     /locus_tag="PK683_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH08873.1"
                     /translation="LPIVEGASVNTKAGPVRTDYILFIAAGAFHISKPSDLIPELQGR
                     FPIRVELEKLSRNDFIKILTDTKSSLTKQYQALLSVEGVELDFSKDGIEELASTAFEV
                     NESQENIGARRLNTILEKVLEDLSFEASDLKPEEKKITVTKEYVQKKIGKIVEDKDLS
                     RYIL"
BASE COUNT          551 a          496 c          407 g          640 t
ORIGIN      
        1 gctttgtgac cgcttccttt tccgaaatcc ttgaaggcat ttctagcttc gagaatctgt
       61 tctacggtca ccggttcgat tttgatcttc aactcgcgga ttagttcatc aaagatacga
      121 gaaagcacag gatcacgcaa ggagtccact cgaatagcag actccagata attggcaccg
      181 gacattgaac attgctctct ctgaatgatc tgcaggaact cttctttctc cggctcagaa
      241 aacagaatag ccagtagagc tgatgaatct acgatcattt tggaagtcca tcctttccat
      301 acagcatctc agaatggtcg gaagactttg cggtcaacgg gacctttgct ttgaattgat
      361 cgaggatttc ctgaattcga actttcctca tcatatcgta gttgtgttcc agacgatcca
      421 gcctttcccg aagagcaatc gtaattgcct gggtaatggt ttcccctgtg agctgagaaa
      481 cctttctcgc caattggtca gtttccggat cttttaaact cagtgccata tatacatata
      541 tatccagtgt atatatttat gcaagaaaat ttcctttttt acttcctgtt taagtcagta
      601 atcagaagag gattttcctt cttgagttcc gaaatcagtt tttccacctc gtccagactg
      661 ggattttttt tcagacgctc cagttctccg gatgtgatgc ctaccatctg cagaaactgc
      721 acctcaccat gcggtgtgtc tattttaccg agttccggat ccaatgcaaa aacaagacca
      781 gtgatttccg tttcagtatt gagccggatc ggaccgtttg caggaatgaa atgattttct
      841 tcaaaccatt tcccgctcga aaaaacatat ctggccagat tgttcatcat ctgaataatc
      901 cagaaaggat ctcccggatc atccttgaag ggaaccagcc tcatcgtaaa ttcaaatccc
      961 cactgactga gctgatcttc gcatgcttct tcattataat ataattcact catcccgtaa
     1021 ctgaccagat gcacgtgagg ggtctgcttt ttgctgtcat agatgctgac accgtcaatc
     1081 ggatctgttc caccggcaat ataatgtata ccgcagggag gaccataatg tctcggctga
     1141 gttttcccgt aaattttctc aatggccgaa tcaatagaaa gccagccgac tgcgtcctcc
     1201 tccgtaaact ttgctttata atcttcgatt ttcataaata ctcaatctgc ataaatccag
     1261 tcaaaatgca aatgttttat tcactcacct tacgcctaac ggctgcaggt ggggcgtttt
     1321 tggcaggaac gcctgccggc ggcttgaggg ctcgcaaggt atggaagcta ctaccgccct
     1381 cgccaaaaag atggctaaac tcccgcaaac ttggaagatg ctgtttttac tgatccatcg
     1441 caaactgtta aactgctaga tttttctaat gctttttgaa aattagagca tctagacagc
     1501 atatcgtatt tgcgtaacgt gagttacaaa gaaaaaaagg gactctttga agagtccctt
     1561 ttgagtaatc aaacagcaat atcagtccat ctgttttcat tacagaatat acctgctcag
     1621 atccttgtcc tctactatct ttcctatttt cttctgaaca tattccttag tcacagtaat
     1681 cttcttttct tcaggcttca gatcagatgc ttcaaagctc aggtcttcga gaaccttttc
     1741 cagaatcgta ttcagtcttc ttgcaccaat gttttcctgt gattcattga cctcaaatgc
     1801 agtggatgca agttcttcaa tcccgtcctt tgaaaaatcc agttccacgc cttccaccga
     1861 aagaagggcc tggtactgtt ttgtcagtga ggattttgta tccgtaagta tttttataaa
     1921 atcgttcctg ctcagttttt cgagttctac acggatcgga aatcttccct gaagttccgg
     1981 aataagatcg gaaggcttgg atatatgaaa tgcaccggct gcaataaaaa gaatatagtc
     2041 cgttctgaca gggcctgctt ttgtatttac ggaagcacct tccacaatcg gaag
//
LOCUS       DAOQGJ010000622         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35913_length_2094_cov_2.995096, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000622 DAOQGJ010000000
VERSION     DAOQGJ010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35913_length_2094_cov_2.995096"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PK683_10255"
     CDS             <1..1166
                     /locus_tag="PK683_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004436615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNH08874.1"
                     /translation="QCEVIGFDGHKYALMPLGEIGGIFPKAFVFSSGKRLTIPTGPSL
                     LGRIINGAGKPIDNKGILMAKEERGPDNEPINPLQRPIISDVLVTGVRAIDGLLTVGR
                     GQRVGIFSGSGVGKSSLLGMIARYTNADINVISLVGERGREVNEFLERDLGEEALEKT
                     VVFVATSDAPKMQQVNCAMLATSAAEYFRDQGLHVNLLMDSLTRFAQASREISISYGE
                     PPITRGYSATVFSKLSRLVERSGTSPGGGTITGFYTILTEPDEMDDPIADAVRGYLDG
                     HILLTRKLAERNFYPAIDVPASLSRVMQTVVGEDHFMYAGFVRELISTYNTSEELILL
                     NAYVKGANPKVDMAIDKKEAIDQFLIQKIETRASYLESLRKLKELFQTPEEDY"
     gene            1166..1747
                     /locus_tag="PK683_10260"
     CDS             1166..1747
                     /locus_tag="PK683_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08875.1"
                     /translation="MKKFKFSLETVMKLRKLKVDEEIRNLSVVVGNINRLNNEIRENT
                     KEISDSVSTFPDLVKSDMKYLKMFDGYIKGLTLKNEQLQRSVSEQDHFLEEARQKVIL
                     ARKDAEIIEIIKRKRHEEFRQKMFRAIRHEEDERNRLDQMEDRRNLKYADKDKDDSKP
                     SVQKQKKQKVSEKPKSDYEKLLEYYEASTKPQR"
     gene            1719..1904
                     /locus_tag="PK683_10265"
     CDS             1719..1904
                     /locus_tag="PK683_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08876.1"
                     /translation="MKPAQNPKDESEFKSFVESMDKETDVRLFRVSKSMVNLSNISIS
                     LEEKVSRLDSLVKSSAE"
     gene            1967..>2094
                     /locus_tag="PK683_10270"
     CDS             1967..>2094
                     /locus_tag="PK683_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HNH08877.1"
                     /translation="MGTMNDRVRAFYLILLIIFLLAIGFFVFDYYKLIDADEIFPF"
BASE COUNT          665 a          351 c          511 g          567 t
ORIGIN      
        1 ttcagtgtga agtgatcgga tttgacggtc acaagtatgc attaatgccg cttggagaaa
       61 ttggaggcat tttccctaaa gccttcgttt tttcttcagg caaaagactt acaattccta
      121 ccgggccgag tcttttgggc agaattatca atggagcagg aaaaccgatt gataataagg
      181 gaattctgat ggcaaaagaa gagagaggtc ctgacaatga accgatcaat cctcttcaaa
      241 gaccgattat cagtgatgta ctagtcacag gggtcagggc aattgacgga cttctgactg
      301 taggtagagg tcagagggtc ggtatttttt ccggttcagg agtgggaaaa tcctcacttc
      361 tagggatgat tgccagatac acaaatgcgg acattaacgt gatttctctc gttggggaaa
      421 ggggcagaga ggtaaatgaa tttctggaaa gggatctcgg agaggaagct cttgaaaaga
      481 cagtcgtgtt tgttgccact tccgatgctc caaaaatgca gcaggtcaac tgcgcaatgc
      541 ttgccacaag tgctgctgaa tacttcaggg atcagggtct gcatgtcaat cttctgatgg
      601 actctctgac cagatttgca caggccagca gggagatttc catatcttac ggagagccgc
      661 cgatcacaag gggttattcc gctaccgtgt tcagtaaact ttccagactg gtggaaaggt
      721 caggaacaag tccgggcggg ggaacaatta ctggttttta caccatcctg accgaaccgg
      781 atgagatgga tgatccgatt gccgatgctg tccgaggtta tctggacggt catattcttc
      841 tgaccagaaa actggcggaa agaaattttt atcctgcgat agatgtgcct gcttcacttt
      901 ccagagtaat gcagactgtt gtgggagaag atcattttat gtatgcagga tttgtccgtg
      961 aactgatcag tacatacaat acctcagaag agcttattct gttaaatgcc tatgtgaagg
     1021 gtgcaaatcc gaaagtcgat atggccattg acaagaagga agcaatagat cagtttctta
     1081 tccagaaaat tgagaccaga gcgagttatc tggaatcact tcgaaaattg aaggagctgt
     1141 ttcagactcc tgaggaagac tattgatgaa aaagtttaaa ttttctctgg aaacagtaat
     1201 gaagctcaga aagctgaaag tagatgaaga gatcaggaat ctttcagtag ttgtaggaaa
     1261 tatcaacaga ctgaacaatg aaatcaggga aaacactaaa gagatttccg attcagtcag
     1321 cacatttccg gatttagtaa aatcagatat gaaatatctg aaaatgtttg acggttatat
     1381 taaagggctt actttgaaaa atgagcagct tcagagatct gtctccgaac aggatcattt
     1441 tctggaagaa gccagacaga aagttatttt agcacggaaa gatgcagaaa ttatcgaaat
     1501 tataaagaga aagagacatg aagagttcag acagaagatg tttcgtgcaa ttcggcatga
     1561 agaagatgaa cgtaacagac ttgatcagat ggaagacagg aggaatttga aatatgctga
     1621 caaagataag gatgattcta aaccgtctgt tcagaaacag aaaaagcaga aagtttcaga
     1681 aaaaccaaaa tcagactatg aaaaactttt ggaatattat gaagccagca caaaacccca
     1741 aagatgagtc tgagtttaag agttttgtgg aatcaatgga taaggaaaca gatgtaaggc
     1801 tgtttcgggt ttcaaagtca atggtgaatc tttccaatat ttctatttct ctggaagaga
     1861 aagtgtccag gctggattct ctggtcaaga gttccgccga ataatttgaa tttttataaa
     1921 aggtacaggc agacctgaat acaggtgcag tcgaagagga gattgaatgg gtaccatgaa
     1981 tgatagggtg agagcctttt atctgatatt gctgattatt tttcttttgg cgataggatt
     2041 ttttgttttt gattattata aactcataga tgcagatgag atttttcctt tttt
//
LOCUS       DAOQGJ010000623         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35917_length_2094_cov_2.853850, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000623 DAOQGJ010000000
VERSION     DAOQGJ010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35917_length_2094_cov_2.853850"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK683_10275"
     CDS             complement(<1..342)
                     /locus_tag="PK683_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08878.1"
                     /translation="MDNLFQTFRPDGFHTVTPYLFADRPELLIDFLKKAFSAKEINRT
                     VNPKNGDTANCILQIGDTCFMISQSGEHFKGMRTALYLFVDDVETVHRRAVENGAMVE
                     FEPDNMPYGDRQ"
     gene            complement(353..1051)
                     /locus_tag="PK683_10280"
     CDS             complement(353..1051)
                     /locus_tag="PK683_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH08879.1"
                     /translation="MKRFQTIKGHIIVYILIGISLFRCSQSHRHHHGSANEHMNQSSF
                     ERLSASFESTGRTKWQKPDEVVRLLGRESRSGSLSGLNVADLGAGTGYFTFRILDANA
                     KVTALDADERFLQFIQKKAESHPKKSSLSVRKIPYTGAELKPDETDILISVNVYHHIE
                     DRVKYFSEIKKALKKNGFICIIDFKEGNLPINSPPESMRISREKTVNELEQAGFKVSV
                     DDKTLDYQYIFIGR"
     gene            complement(1048..1656)
                     /locus_tag="PK683_10285"
     CDS             complement(1048..1656)
                     /locus_tag="PK683_10285"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNH08880.1"
                     /translation="MKKNTYTAALFLIITLYCNRQENNDAEKKHPEKGLNPQEIASQA
                     VGKAKAELGKNLLNAISSKGAAYAVSFCSEKALALTEEMSKQYNVRLKRVSDRPRNQS
                     NLANDEELTVIKNFKTQLEKKEKLTPAFRETETEFIGYFPIETNQMCMQCHGKKDTDI
                     HSSVYENILKIYPEDKAVGYSENQIRGIWTVMIQKKNNEGYR"
BASE COUNT          536 a          421 c          387 g          750 t
ORIGIN      
        1 ctgtctgtct ccataaggca tattgtccgg ttcgaactcc accattgctc cgttttcgac
       61 agctcttctg tgaacagttt ctacatcatc aacaaaaaga tacaatgctg ttctcatccc
      121 tttaaaatgt tctcccgact ggctgatcat aaagcaggta tctccaatct gaagaatgca
      181 gtttgcggta tcaccgttct taggattgac tgtacggttt atttcctttg cagaaaatgc
      241 cttttttaaa aagtcaatta aaagctcggg tcggtcggca aagagatagg gcgtgactgt
      301 atgaaatccg tcaggtctga atgtctgaaa aagattgtcc atattcaata agctatcggc
      361 cgatgaagat atattgataa tctaaagttt tatcatcaac ggagaccttg aatccggctt
      421 gttccagttc gtttacagtt ttttccctgg aaattctcat agattcaggc ggactgttaa
      481 tcggaagatt tccttccttg aagtcaataa tgcatataaa tccatttttt ttcagtgctt
      541 ttttaatttc agaaaaatac ttcactctgt cttcaatatg gtgatacaca ttcacggata
      601 tcagaatgtc tgtttcatca ggtttcagct ctgcaccggt atagggaatt tttctgactg
      661 acagactgct ctttttcgga tgagattctg ctttcttctg aataaactgc agaaatcttt
      721 catctgcatc aagtgctgtc acctttgcat tcgcatccag aatccgaaat gtaaaataac
      781 cagtgcctgc gccaagatcc gcaacattca gaccggaaag agatccggat ctgctttccc
      841 tgccaagcag tctgacgact tcatccggtt tctgccattt tgttcttcca gtactttcga
      901 aggatgcaga aagtctttca aatgaagact gattcatatg ctcatttgct gaaccgtgat
      961 gatgtctgtg tgattgagaa cagcggaata atgatatacc gataagaata taaacgatta
     1021 tatgtccttt tattgtctga aatcttttca tctgtaacct tcattatttt tcttctggat
     1081 cataacagtc catatgcccc gaatctgatt ttcggaatag cctacagcct tgtcttccgg
     1141 ataaattttc aatatgtttt cgtatacact ggaatggata tcagtgtctt tttttccatg
     1201 acactgcata cacatctggt ttgtttcaat tggaaaataa ccgatgaatt cagtttcagt
     1261 ttctctgaag gcaggagtga gtttttcctt tttttcaagc tgtgttttaa agtttttaat
     1321 gactgttaat tcttcgtcat ttgcaagatt tgactggttt ctcggtctgt ccgaaaccct
     1381 cttcaagcgg acattgtact gttttgacat ttcctccgta agagccagtg ctttttcact
     1441 gcagaaactg actgcatatg cggctccctt ggaagaaatg gcattcagaa gatttttccc
     1501 cagttccgcc tttgctttgc ccactgcctg agaggcaatt tcctggggat tcaggccctt
     1561 ttcgggatgt tttttttctg catcattatt ttcctgtctg ttgcagtata atgtaattat
     1621 aagaaagagt gctgcagtgt aggtgttttt tttcataatt tcctctgaca gtataattaa
     1681 tgcagtcaca ctgtatgcaa atgttcttca aaagtaatga tgcttttttt ctttattctg
     1741 aatcgaggat tttttttcaa tttcaaattc ttctgtaaat ttcaaattga gattaattgg
     1801 atttgtacaa atttctcatt tgtttttaac ctgtttaaac ggtctccttg catcagtttt
     1861 tcagattgag ttatgcactt ttttgaatcc ttctccaggc tgtctaagct tcagtcttct
     1921 ttgcttggcg gaaaacgctg attttcaact gtatataagg cttttactgc acagagagga
     1981 gattttaaag ggagttttaa tcatctgtct gttgattttc gctgactgaa atcaaaaatc
     2041 cgcccaaatg tgaggaaatt cagacagctc ttctgcagta cattgtcttt ttcc
//
LOCUS       DAOQGJ010000624         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_35998_length_2091_cov_2.408644, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000624 DAOQGJ010000000
VERSION     DAOQGJ010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35998_length_2091_cov_2.408644"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PK683_10290"
     CDS             complement(<1..603)
                     /locus_tag="PK683_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNH08881.1"
                     /translation="MNLRKSFITISGLIIYLTYGMLSAVPVKEDRLKVESGKMLFTLK
                     KKNSSGYDPDMVKSETMTGMANRASGTVNMKDRTFDITMDVGLETFFLGGKFKFANTR
                     MHENYLESSKYSSVNYKGTIVSYDEESGKAKVKGKMTLHGVSKDNFSTEGTVTVSKSG
                     KGYLLTVSFNVNLRDFGIQVPNTKITKIREMVALKVKLELV"
     gene            complement(1114..>2091)
                     /locus_tag="PK683_10295"
     CDS             complement(1114..>2091)
                     /locus_tag="PK683_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08882.1"
                     /translation="LSEELYGADEKLITRTVYKYDEKGNLLELESFGADGKYQGDNKG
                     IARYVIQYDENCINLKKDSLTCCSLSEYYDKDGRLIGDGIYQTPRTVRKFDKNANEIE
                     TEFYGTDGKFQEGKIARQVFKYSDTGNKLLEEFYGSDGRLFEVLGIARKVYKYDENCL
                     NFTAHAYVCRTLEENQGADGSLRGDFYNIGRTVFAFDENCIRIKKIGNECYSKIEYFG
                     VDGKLKEKIGAAIERNKYDSDGNRILQEYYGKDGKLKGEGAAARITAKYDKNGYKISS
                     TSFDSSGSVIEQIFSPVFTHPSFPGKVFQLVLDEYFLLHSVKLEDAKKN"
BASE COUNT          556 a          479 c          367 g          689 t
ORIGIN      
        1 aaccagttcc agtttgactt tcagcgccac catttcgcgg atctttgtaa tctttgtatt
       61 cggcacctgg attccaaaat ctctcagatt tacattgaag gaaacagtca gaagatatcc
      121 tttccctgat ttggatacgg ttactgttcc ttctgtgctg aaattgtcct tggaaactcc
      181 gtgaagagtc atttttcctt ttacttttgc ctttcctgat tcttcatcat aagatactat
      241 ggtgccttta taatttactg aactgtattt ggaggattcc aggtaattct cgtgcatcct
      301 tgtatttgca aacttgaatt ttcctcccag gaaaaaagtt tcaagcccga catccattgt
      361 aatgtcgaaa gtcctgtctt tcatattgac agtgccggag gcacggtttg ccatccccgt
      421 catcgtctcc gacttcacca tatccggatc atatcccgag gaatttttct tcttcagagt
      481 aaacagcatt ttgccggatt ccaccttgag cctgtcttcc ttgacgggaa ctgccgaaag
      541 cattccatat gtcaaatata taatcaatcc tgatatagta ataaaacttt ttcttaaatt
      601 catccgtttc tccttaaaaa aactgttttc atatgcacag aaaatctttc taatgcagag
      661 cagccctctg cccgacagtt tcactgctga aaacccattt cataagagca tatacatttg
      721 actgtatgct ccactgcttt cataatgaat atttgatgca cattgtcaag gcttttaaat
      781 ttttgcatcg gaatgcccgt actgataaaa gtcatatatc ctgcagacag cagggaagtt
      841 tttatgctga aaaagaaatc aggccttgcg gaaaaatatt gaccttaccg aattctgctt
      901 gcctgaataa agttcagttt tgacagtcgt cttatgctgc agatcgccta cggtgagtcc
      961 aatttcgaga atttaggaaa gagaactccg ctcaattgca atgattgtct ctcatacaaa
     1021 agaagtggag tttctgaaga tcgcaggctt ttcagaaaat gaaaccctgc aaccaaggct
     1081 gtagttccga acgactctta aagcagaccc aaatcagttc ttcttcgcat cttccagctt
     1141 cactgagtgg agaaggaaat attcatccag taccagctga aagactttgc cgggaaagga
     1201 gggatgagta aaaacaggag agaagatttg ttcgataact gaaccggagg aatcgaaaga
     1261 tgtcgaggat attttatatc cattcttatc atattttgca gtgattcttg cggctgctcc
     1321 ttctcctttc agttttccgt ccttcccgta atattcctga agaatgcggt ttccgtctga
     1381 atcatattta ttccgttcta ttgcagcccc gatcttttct ttaagctttc catccacacc
     1441 gaaatattcg atttttgaat aacattcatt tccgattttc tttatacgaa tgcagttctc
     1501 gtcaaacgca aagacagtcc ggcctatgtt ataaaagtct ccccgcagac ttccgtctgc
     1561 tccctgattt tcctctaaag tcctgcaaac gtatgcgtgt gccgtgaaat tcaggcagtt
     1621 ctcatcatat ttatatactt ttcgcgcgat gcccaatact tcgaaaagcc tgccgtccga
     1681 tccataaaat tcttccagca gcttatttcc cgtatccgaa tatttaaaca cttggcgggc
     1741 aattttgcct tcttgaaatt ttccatcagt tccgtaaaac tcagtttcaa tttcgtttgc
     1801 atttttatcg aatttgcgta cagtacgggg ggtttgatag attccatccc caatcaatct
     1861 gccgtctttg tcataatatt ctgataagga acagcaggtc agcgaatctt ttttgagatt
     1921 aatgcagttt tcatcatatt gtattacata ccgggcaatt cctttattat ccccctggta
     1981 ttttccatcg gctccaaagc tctccagttc taaaagattt cctttttcat catatttata
     2041 aacagtcctg gtaataagtt tttcatcggc tccatataat tcttccgaca g
//
LOCUS       DAOQGJ010000625         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36022_length_2090_cov_3.702211, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000625 DAOQGJ010000000
VERSION     DAOQGJ010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36022_length_2090_cov_3.702211"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..567
                     /locus_tag="PK683_10300"
     CDS             343..567
                     /locus_tag="PK683_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08883.1"
                     /translation="MLQRTSPLNTPEYRDMIENLELGSETAEGASLRLNMNRNQLKVL
                     RFRARQQLKKFMEESCRVCAKHGCLDCTCS"
     gene            complement(579..1154)
                     /locus_tag="PK683_10305"
     CDS             complement(579..1154)
                     /locus_tag="PK683_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08884.1"
                     /translation="MKELQDRFTETEWMCITFSPICACYAIVFSDMRNETDKEFEVLF
                     NSVEKYRQEYSKNSLVQSALSTFDGTEAFKKNLDRMQKFPEEIETNKEIIALAQLNSK
                     IIQFVKDAKTRSVGGPVEETMNACVIIIGCANYILKGKNIPERIEFNRMLMGIATDVA
                     SAVKEGGMLGFGKSISEKEEYILECLNKILE"
     gene            complement(1210..1569)
                     /locus_tag="PK683_10310"
     CDS             complement(1210..1569)
                     /locus_tag="PK683_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH08885.1"
                     /translation="MNISSGKTVFFLWISLLSFCGDGGKSHSWEVASKVHSGAKIVDV
                     RTAHEFSKGHYPGAVNIPVEEIEDRIHSLESYKHRTVVLYCESGQRAGKAKKILEANG
                     FRTVYNAGGLEDMPAVK"
BASE COUNT          584 a          426 c          389 g          691 t
ORIGIN      
        1 aatcatgctt gtggttgccc cgttgaatcc ttttttggaa ataatgtcta cagcggcaag
       61 aaatacaagc ttctgttttt cagtcatatc tccgaagtcg atttccggcg gttcggggaa
      121 aggagctttc ttttttcttt cagttttcat aggtgagtga ttactcactt tattttttcc
      181 cagaaaaatg tcaataagaa ttctgtcatg gttttttacg gttctcagaa atgctgtaat
      241 tgacagcgtc aggaagacga attctgaaga gaaaaagacg gaatcaatga aacacttctt
      301 atcattaaat tataattctg aggatgagtc tgaaatctgt gagtgcttca aagaacttct
      361 cccctcaata cacctgaata cagagatatg attgaaaacc tggaacttgg cagcgaaaca
      421 gcagaaggtg cctctctcag actgaatatg aacagaaacc agttgaaagt gcttcggttc
      481 agagcaagac agcagctgaa aaaatttatg gaagaatcct gcagggtatg cgcaaaacac
      541 ggatgcctcg actgcacctg cagttaaata gagaattctt attccagaat cttattcagg
      601 cattcaagga tatattcttc cttttcactg atgctttttc caaatccaag cattccgcct
      661 tcttttactg cggaagcaac gtctgtcgca atacccatca gcattctgtt gaattcaatt
      721 ctttcaggga tgttttttcc cttcaaaata taatttgcac atcctataat aataacacat
      781 gcattcattg tttcttccac aggtccgccg actgaacgag tcttggcgtc cttgacaaat
      841 tgaatgattt ttgaattcag ctgtgcaagg gcgatgatct ctttgtttgt ttcaatttcc
      901 tcaggaaatt tctgcattct gtcaagattt tttttgaatg cttctgtccc gtcaaaagtg
      961 ctgagagcgc tttgaaccag ggaatttttc gagtattcct gtctgtattt ttcgactgag
     1021 ttgaaaagca cttcaaattc tttgtcagtc tcatttctca tatccgaaaa cacaatggca
     1081 taacatgcac agatcggaga aaaggtgatg cacatccatt ctgtttctgt aaatctgtcc
     1141 tgcagttctt tcatacagtc ctccaaaagt cagtccattg aattacggca gacttgctct
     1201 gtaaatcctt tatttcactg caggcatatc ttccagaccg cctgcattgt atactgtcct
     1261 aaaaccgttt gcttccagta ttttctttgc cttacctgct ctctgtcctg attcacagta
     1321 cagcactaca gtcctgtgct tatatgattc gagggaatgg attctgtctt caatttcctc
     1381 tacaggaata ttgactgctc ccggatagtg acctttactg aattcatgag ctgttcttac
     1441 atcgactatt ttagctcctg aatgaacctt gcttgctact tcccaactgt gactttttcc
     1501 gccgtcgccg cagaatgaca gcaaggaaat ccatagaaaa aagactgttt ttcctgatga
     1561 tatgttcatt gtattatatc cttaatatga atattcggga tgtcgaggtg cctgtatact
     1621 gaaaaagagc ttcctgcttt aaaaaaactc aaaatttctc tcaacttgtc tatctccgct
     1681 cctgcacagt taagcttatg cagctgaact gaatgcagat cggctctgtg ctcagccgat
     1741 atgacaatta tcaatttatt cagtgataat ttttgctgtc tcaatgagtg atcatattac
     1801 tggaatgcaa gtttttaaat tttactcaaa gtgaagaggc tttctatgaa aaaatatcta
     1861 cggtgtttgt tttcagtatg ttttattttt tcgctcagtg tactgaaagt aaaaaagcag
     1921 tgaaaaaaga aatctgggac tggggattct cctgttgagt gcatctgatt ccattctgaa
     1981 attcagtgga gcttcgttca caaaggacag cggtcagtgc ctgagaaatc aatgatgaac
     2041 tctctgaaat taaatataat taaattctat tttaatttca ggattcatta
//
LOCUS       DAOQGJ010000626         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36030_length_2090_cov_2.992629, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000626 DAOQGJ010000000
VERSION     DAOQGJ010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36030_length_2090_cov_2.992629"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PK683_10315"
     CDS             <1..264
                     /locus_tag="PK683_10315"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNH08886.1"
                     /translation="AELPQMRSFVLPGGTKAASMLHIARTVCRRLERQMTELQENAGT
                     VSPEALIYINRLSDYLFTAARYANFKSGNEDVPWKSRAKANAG"
     gene            254..1516
                     /locus_tag="PK683_10320"
     CDS             254..1516
                     /locus_tag="PK683_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNH08887.1"
                     /translation="MQDKHHRALPILFFTEMWERFSYYGMRGLLIFYLTKHLLMSDAA
                     AGKIYGAYTALVYLAPLLGGYAADNILGRIKSVYIGGILMMIGHFSLALEGIFFFYAG
                     LAFLIFGNGFFKPNMSSLLGELYTEKQTLRESAYTIFYMGINIGGAFGPLLCGYIGEK
                     YGWHYGFSLAGFGMFIGLVQFYFGRGLIGNVGEFKPSAVSEHRDVPLSRSEKNSLVYL
                     AVLGFFALFFWIPYEQMGSAVSLFTDRKTDRFVFGTEIPASMFQSVNGFMILMFAPIT
                     AGIWTALSKEKKDLAISSKFILAVILLGLSYLVLYFGEIFTGNAKTPLIYLMLYYALL
                     TFGELCISPVGLSMAVRLAPVKFSVTVVGFWFTANAVSHYVSGWLSGAYSKWFSVHDF
                     FLFLALVCFGSAVILSVFSRKLEELLNE"
     gene            1509..>2090
                     /locus_tag="PK683_10325"
     CDS             1509..>2090
                     /locus_tag="PK683_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08888.1"
                     /translation="MSKYRSNLFAFLKKLKRPFFFSLFLQISSVLLNTSFSLMGFDIS
                     ETVRTYFNFQFAFVFLSASVKIFFIYFIFFTFLQLLFERFFEKYRLPGFSSYLLFSFF
                     VFVFFIESVGRYPQMYSDFFYGKHSYLKFLLHFTTDYISPDIVRIFVIMILALPAVIQ
                     MILSLKSPDFSNVSLSVFSVFLVYLHYEGYSVII"
BASE COUNT          494 a          423 c          455 g          718 t
ORIGIN      
        1 gctgaactgc ctcagatgag atcctttgtt ctgccgggcg gaactaaggc agcatcgatg
       61 cttcatatcg caaggacagt gtgcagaaga ctggaaagac agatgaccga acttcaggaa
      121 aatgcaggaa cagtgagtcc tgaggcattg atttacataa acaggctttc tgattatctt
      181 tttactgcgg ccagatatgc gaatttcaag tcaggaaacg aggatgttcc ctggaagtca
      241 agggcaaagg caaatgcagg ataagcatca cagagctctt cccatacttt tttttacaga
      301 gatgtgggaa cgattcagct actacggaat gagaggactc ctcatttttt atcttacaaa
      361 acatctgctg atgagcgatg cagccgcagg aaaaatttac ggtgcatata ctgcactggt
      421 gtatctagcc cctctgctcg gagggtatgc tgcagacaat attctgggaa ggatcaagtc
      481 agtttacatc ggcggaatac tgatgatgat cgggcatttt tcccttgctc tggagggcat
      541 ttttttcttt tacgcaggac tcgcctttct tatattcggg aacggttttt tcaaacctaa
      601 tatgagttct cttctcggtg aactgtatac ggaaaagcag actctcagag aatcagcata
      661 tacaattttt tatatgggaa ttaatatagg aggagcattt ggtcctcttc tctgcggata
      721 cattggagaa aaatacggat ggcattacgg cttcagtctg gctggattcg gaatgttcat
      781 cggtctggtt cagttttatt tcggaagagg actgatcgga aacgtcggtg agttcaagcc
      841 gtcggctgtt tcagagcata gggatgttcc tctgagcaga tctgagaaga acagtcttgt
      901 atatcttgca gttctgggtt tttttgcact ttttttctgg ataccctatg aacagatggg
      961 ttcagcagtc agtctgttta cagacaggaa gacagacaga ttcgtgttcg gaactgagat
     1021 tcctgcatct atgtttcagt ctgtaaacgg ttttatgata ctgatgtttg ctccgatcac
     1081 tgcaggcatc tggacagcgc tttcaaagga aaagaaagat ctggcaatat cctccaaatt
     1141 catacttgcc gtcatattgc tggggctttc ctatctggtc ctttatttcg gcgaaatatt
     1201 taccggaaat gcaaaaactc cattaatata tcttatgcta tattatgctc tgcttacttt
     1261 cggtgaactc tgtatctctc ctgtcgggct ttccatggca gtgcggcttg ctccggtcaa
     1321 gttttccgta actgtggtcg gcttctggtt cactgcaaat gcggtatcac attatgtttc
     1381 gggatggctt tcgggcgctt attccaaatg gttttctgtc cacgattttt ttctgtttct
     1441 cgcacttgta tgtttcggat ctgccgtaat tctgtccgtt ttcagcagaa agctggagga
     1501 gcttctgaat gagtaaatac aggtcaaatc ttttcgcatt cctgaaaaaa ctgaaaaggc
     1561 cgtttttctt cagtcttttt ctgcagatct cttccgttct gctcaatacc agtttcagtc
     1621 ttatgggctt tgatatttct gaaacggtcc gaacgtattt caattttcag tttgcatttg
     1681 tgtttctttc tgcatctgtg aaaatatttt ttatttattt tatatttttc acctttcttc
     1741 agcttctttt cgagcggttc ttcgaaaaat acagacttcc gggattttcc agttatctgc
     1801 tcttcagttt ttttgtattc gtatttttca ttgaatcagt cggaagatac cctcagatgt
     1861 actctgattt cttttacgga aagcattctt atctgaagtt tctgctccat tttactaccg
     1921 attatatttc ccccgatatt gtcaggatat ttgtcataat gattcttgct ctgccggcag
     1981 tcattcagat gattctgtcg cttaagagtc ctgatttttc aaatgtttca ttgtctgttt
     2041 tctctgtgtt tcttgtgtac ctgcactatg agggatattc tgtcattatc
//
LOCUS       DAOQGJ010000627         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36056_length_2089_cov_3.105211, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000627 DAOQGJ010000000
VERSION     DAOQGJ010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36056_length_2089_cov_3.105211"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PK683_10330"
     CDS             complement(<1..1248)
                     /locus_tag="PK683_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08889.1"
                     /translation="MISLSEFLKTKPIETPEAFSVKSEKKTAFSENSDVVESKKPFQE
                     FLKEASQEKTPAQSVAVAVPAEITVEETPEPEDDSISSEDESEEEGLEIHLEKGESEE
                     PAVESVKDLRVRLAEDSAEIIEKNPKQSKETVFERKESDSLEKDSIETFAVSESEETD
                     FSSEKQKVSFRDSFFQNEEIANKEEVVSEMFSIRNEKEGVEILTENNRKEEDLLLSLG
                     KEKNLKQIPSEKKDKEIISAKEPEKQPVPPSERIAQQPESSASVFSNAVQKEPTYQDL
                     LKKLSSEAKRIQTPLKEKLSEKEKTESSENPKSVQLSVLHSKEKKTDSEMNMKETVQT
                     RKETARLPEEALQIKNSKETSLPVQNTESSPRVEDRQIPLFQSLRQNRTEKSNAELSF
                     VSNPKEVLKNNFSEILKSVRTNIL"
     gene            1344..1775
                     /gene="flgB"
                     /locus_tag="PK683_10335"
     CDS             1344..1775
                     /gene="flgB"
                     /locus_tag="PK683_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNH08890.1"
                     /translation="MFESTYFMKTQTVLEKGLGASMLKRQVLSDNIANVDVPHFKRSE
                     VLFEEQLKRAIESESTEKQKAVPTLTTDSRHIEFFKSQDFREVHPKANIDYLTSMRAD
                     GNNVDMEKEVTEASHNQMIYSLMIERLNQNFRQLHTVMRTV"
     gene            1775..>2089
                     /locus_tag="PK683_10340"
     CDS             1775..>2089
                     /locus_tag="PK683_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HNH08891.1"
                     /translation="MGLFTSINISSSGLSAQRLRMDVISNNIANSTTTRNTNGDGPFR
                     RDRVVMSPINMRTRWRSPVYPFGLATGEGKGVKVLKVEKDMSPLRLVYDPSHPDSIQV
                     GEK"
BASE COUNT          560 a          442 c          426 g          661 t
ORIGIN      
        1 cagaatattt gttcttacgg atttgagtat ttcggaaaaa ttatttttca gcacttcctt
       61 agggttggat acaaaggaaa gctcagcatt ggatttttct gtcctgttct gtctcaggga
      121 ttgaaaaaga gggatctgcc tgtcctctac tcggggcgat gattctgtgt tctgaacggg
      181 aagagaagtt tccttggaat ttttgatctg aagagcttct tcaggaagac gggcagtctc
      241 ttttcttgtc tgcacggtct ccttcatatt catctcagag tctgtctttt tttctttgga
      301 atgtaagacg gaaagctgaa ctgattttgg attttcggaa ctctcagtct tttccttctc
      361 cgaaagtttt tctttcagag gagtttggat tcgtttggct tcactcgaaa gtttcttcag
      421 cagatcctga taagtcggtt ccttctgcac tgcatttgaa aacactgagg cactgctttc
      481 cggctgctgt gcaattcttt cggaaggagg aacaggctgt ttttcgggtt ctttggcaga
      541 gatgatttct ttatcttttt tttcagaagg gatctgtttc agattttttt cttttcccag
      601 acttaaaagg agatcctctt cttttctatt gttctcggtg aggatttcga caccctcttt
      661 ttcatttcga atggagaaca tttcagagac aacttcttcc ttatttgcaa tttcttcatt
      721 ttgaaagaag gaatcgcgga aagagacttt ttgtttttcc gaagaaaaat ctgtttcctc
      781 tgattcagaa acggcgaatg tctctatgga atctttttcc aacgaatcag attcttttct
      841 ctcaaaaaca gtctctttgg actgtttcgg gttcttctca atgatttccg ccgaatcttc
      901 agcaagtctg actctgagat cctttactga ttcaaccgca ggctcctccg attcgccttt
      961 ttccagatga atttcgagcc cctcttcctc ggattcatcc tcagaagaaa tagagtcatc
     1021 ctcaggctca ggggtttcct cgacagtaat ctctgcagga acagccactg caacggactg
     1081 cgcaggagtt ttttcctgtg atgcttcctt cagaaattcc tgaaatggct ttttggactc
     1141 caccacatca gaattctcgg aaaatgcagt ttttttctcg gattttactg aaaatgcttc
     1201 cggagtttcg atcggttttg tttttagaaa ttctgaaagg gaaatcatag tgtctctatt
     1261 ctattttcgc tatttttcgc acttgcttaa cttttttttc taaaagaaaa acttttctga
     1321 ccgaaattac atttgagggt attatgttcg aatctaccta ttttatgaaa actcagacgg
     1381 ttctggaaaa agggctcggc gcatccatgc tcaaaagaca ggttctctct gacaacattg
     1441 caaatgtgga tgtcccccac ttcaaaagaa gtgaagttct ctttgaagag cagctgaaaa
     1501 gagcaattga atcagaaagc actgaaaaac agaaagctgt tccaaccctc acgacagact
     1561 ccagacatat tgaatttttc aaatctcagg acttcagaga agtacatcca aaagcaaaca
     1621 tcgactatct gacttccatg agagcggacg gaaacaatgt ggatatggaa aaagaagtca
     1681 cagaagcatc tcataatcag atgatctact cactgatgat tgaaagactg aaccagaact
     1741 tccgtcagct ccatacagta atgaggactg tataatgggg ctttttacat ccataaatat
     1801 ttcatccagc ggcctttctg cacagagact cagaatggac gtaatttcca acaatatagc
     1861 caattcgact acaacgagaa ataccaacgg tgacggccct ttcagaagag atcgggtggt
     1921 aatgagtccg atcaatatgc gcaccagatg gagaagtcct gtatatccat tcggacttgc
     1981 cacaggtgaa ggaaagggag taaaagtact taaagtagaa aaggatatgt cccctcttcg
     2041 gctggtctat gacccatctc accctgactc cattcaggtc ggagaaaaa
//
LOCUS       DAOQGJ010000628         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36066_length_2089_cov_2.307768, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000628 DAOQGJ010000000
VERSION     DAOQGJ010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36066_length_2089_cov_2.307768"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PK683_10345"
     CDS             complement(<1..559)
                     /locus_tag="PK683_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08892.1"
                     /translation="MKYTETELRDIIDKGTQKNTIISMKTYLLSDYGELILKIISESI
                     LRKFTRLDLMEIMYSAAKELVINATKANVKRVLFRQLNLDIQNPEDYEKGIEVFKESL
                     TEDKITTMKQDFKKYDLPVTATFYYNEDVINIKVKNNFPLIEEEEKRIRDKFRKATSF
                     SSLIDFFMEYGDNTEGAGMGITMVGI"
     gene            675..917
                     /locus_tag="PK683_10350"
     CDS             675..917
                     /locus_tag="PK683_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08893.1"
                     /translation="MQADPGHIFLELNRPENNSGIKSLGETMKKIFTTVLALAMALSL
                     TYCSSEEKKAETAAPAKEEVKAPAVPAKTPAPAKKK"
     gene            complement(1004..>2089)
                     /locus_tag="PK683_10355"
     CDS             complement(1004..>2089)
                     /locus_tag="PK683_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH08894.1"
                     /translation="PMNGVIGMIGLLLDTNLNEEQRQYAEIVKNSAESLLSLLNDILD
                     FSKIESGKVILEDRVFLIQKTIENLYYLLEKAALLRKNQLVIQFTETFPKFISGDEIR
                     IRQILLNILSNAVKFTAEGKITLTGKLKEENGETLTLEFEVTDTGIGISAEKIEMIFE
                     AFSQADESTSRVFGGTGLGLSITKKLIELMQGKISVTSEIGKGSVFTFTVKCRRAENM
                     EEGTQTGSVSFAGYAGFADQYPARILIAEDNLANQKLICRIFEKFGYSAELAYNGREA
                     LEAAENRDFDFIFMDIQMPFMDGYRTTEKILSSPKIKKKPKIFAMTANAFEEDKKKCL
                     ETGMQDYISKPFKINEISELIIKWYGK"
BASE COUNT          558 a          436 c          357 g          738 t
ORIGIN      
        1 gaatgccgac cattgtaatc cccatacctg caccttctgt attgtctcca tattccataa
       61 agaaatcaat caggcttgaa aagctggtag cttttctgaa tttgtcgcgg attcgttttt
      121 cttcctcttc gatcagcgga aaattgtttt ttaccttaat attgattaca tcttcattgt
      181 aataaaatgt tgcagtcaca ggcagatcgt attttttgaa gtcctgtttc atcgtggtga
      241 ttttatcctc agtcagactt tctttgaaca cttcaattcc cttttcatag tcttccggat
      301 tctggatgtc cagattcagc tgacggaaaa ggactctttt tacattggcc tttgttgcat
      361 tgatgacaag ttcttttgcc gcggaataca taatttccat gagatcaagt cttgtaaatt
      421 tccttaaaat gctctcagaa atgattttca gaatcagctc gccataatca ctcagaaggt
      481 acgtcttcat tgatatgatc gtgttttttt gtgtaccttt gtctataatg tccctgagtt
      541 ctgtttccgt gtatttcaaa atttcctctt ttgctcgcaa acaaggtaat acgaatagat
      601 tttctgtcaa ttcctttgcg taaaaaatag ctttttttga gggaatctgt acgacagttg
      661 aacaggtgta acgtttgcag gcagacccag gccatatttt tcttgaatta aaccggccgg
      721 aaaataattc tggcattaaa tcattaggag aaactatgaa aaaaattttt acaactgttt
      781 tagctctggc aatggcactg agcctcactt actgctcttc agaagaaaaa aaagctgaga
      841 ctgcagcacc tgcaaaagaa gaagttaagg ctcctgcagt tcctgctaaa actccagcac
      901 cagctaagaa aaaataattt tcttggattt tactggctgt gtaaactgtg attctgtgaa
      961 tatacgcgga tgctgtttac acagcctacc tattgacagg tctctatttc ccataccatt
     1021 ttattatcag ttctgatatt tcattgatct taaacggttt gctgatataa tcctgcattc
     1081 ctgtttccag acacttcttt ttgtcttctt caaatgcatt cgcagtcatc gcaaaaattt
     1141 taggtttctt tttaattttt ggagaagaga ggattttttc cgtcgttctg tatccgtcca
     1201 taaacggcat ttgtatatcc ataaaaataa agtcaaaatc cctgttttcg gctgcttcca
     1261 gtgcctctct tccattgtat gccagttcag ctgaataacc gaacttttca aatatcctgc
     1321 agatcagttt ctgatttgcc aggttatcct ctgcaatcag aattcttgca ggatactgat
     1381 cagcaaagcc cgcataacct gcaaaagaaa cagagccagt ctgtgttcct tcttccatat
     1441 tctccgctct tctgcacttt actgtaaaag tgaatacgga accttttccg atctcgcttg
     1501 tcacggagat ttttccctgc atcagttcga tcagtttctt tgtaatggat agtccgaggc
     1561 ctgttccgcc gaaaacccgt gatgtagatt catcagcctg agaaaatgcc tcaaagatca
     1621 tttctatttt ttctgctgaa ataccgatgc ctgtatccgt gacctcaaat tccagtgtaa
     1681 gagtttcacc gttttcttcc ttcagttttc cggtaagagt gatctttccc tcagcagtaa
     1741 attttactgc attgctcagt atgttcagaa gaatctgtct gattctgatt tcatctcctg
     1801 aaatgaattt tgggaatgtt tccgtaaact gaatgacaag ctgatttttt ctgagcagtg
     1861 ctgctttttc aagaaggtag taaaggtttt caattgtttt ctgaatcaga aaaactctgt
     1921 cttccagtat gacctttcct gattctattt tagagaaatc taatatatca tttaacagag
     1981 ataaaagaga ttctgcacta ttttttacta tttctgcgta ctgtctttgt tcttcattta
     2041 aattagtatc caataataaa ccaatcattc ctattacacc attcatagg
//
LOCUS       DAOQGJ010000629         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36119_length_2087_cov_3.963091, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000629 DAOQGJ010000000
VERSION     DAOQGJ010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36119_length_2087_cov_3.963091"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="PK683_10360"
     CDS             <1..2047
                     /locus_tag="PK683_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH08895.1"
                     /translation="KDENGTIVNFIGIKDNITEEREIREKLVESEKKLRTLFNSLEVG
                     ITITDKEGNIIDCNPASEKILGISREEHITRNYAGKEWKILRTDFSLMPSEEYASVRA
                     MKENRLVANVEMGIVKSNETVSWINVTAIPLNMPNYGVVITYTDITEVKQKEDALQRA
                     LNLFKSAEIQNRLILKNAGEGIFGLDENGKTVFCNDACCSMLGYTAEELIGKEIHSLI
                     HHSYPDGSPYPSQKCPMFDCVTQGKQSRHSDDFLWKKEGVCFPVEYITTPIYNNDKII
                     GAVVIFSDITERKKYEEDLKKAKDEAEKANRLKSEFLANMSHEIRTPMNTVIGFAEIV
                     REKTSNNPEIQDYMEGIQKSGKTLISLINNILDLSKIESGLMEISKGPVNLKSAVSDT
                     VQMFSLQAKEKGIHLYQKLDRDLPDGFYLDQTRMRQILLNLTGNAIKFTDKGEVCISV
                     SAEQSLEDKNEYTLKMEVTDTGSGIAETEIDRIFHPFIQQKGQDMSRYGGSGLGLSIT
                     KKLTELMGGSLFVESVMGQGSNFTVILKKVRTAELVNIRSNNQFFDINHIPSLNGRIL
                     LVEDVASNRKVVNGLLRKTGIQILEAENGRKAVEILEKEKVDLILMDMHMPEMGGQEA
                     SIVIKSRPEWSAIPIIILTADAMSDNIESFKEFSEGYLTKPVTKEDLIFTLAKFLGE"
BASE COUNT          726 a          346 c          497 g          518 t
ORIGIN      
        1 caaggatgag aatggaacaa ttgtaaattt tatcggcatc aaggacaata ttacagaaga
       61 aagagagatc agggaaaagc ttgtagagag cgaaaaaaaa ctgagaactc tttttaattc
      121 ccttgaagta ggcataacga tcacagacaa agaaggcaat attattgact gcaatcctgc
      181 atccgaaaaa atattgggca tttccaggga agagcatatt accagaaatt atgcaggaaa
      241 ggaatggaag attctgagaa cggacttttc cttaatgcct tcggaggagt atgcaagcgt
      301 aagagcaatg aaagagaaca ggcttgttgc caatgtggaa atgggaatag tgaagtccaa
      361 tgagactgtg agctggataa acgtgaccgc aattcctctg aatatgccaa actatggtgt
      421 tgtgattaca tatacagata ttactgaggt taaacaaaag gaagatgctc tgcagagagc
      481 actcaactta tttaagtctg cagaaataca gaatcgtctg atcctgaaaa atgccgggga
      541 gggaatattc ggtctggatg aaaatggaaa aactgttttc tgcaatgatg cctgctgcag
      601 tatgctcggt tatacagcgg aagagctgat tggaaaagaa attcattcac tgattcatca
      661 cagttatcca gacggaagtc cgtatccctc gcaaaaatgc cctatgtttg actgcgttac
      721 gcagggaaaa caaagcaggc attcagacga ttttctctgg aaaaaggaag gtgtctgctt
      781 tccggtggag tatattacaa cacctatata caataatgat aaaataattg gggctgtagt
      841 catcttcagt gatattacgg aaagaaagaa gtatgaggaa gatctgaaga aagcgaaaga
      901 tgaagcagaa aaagccaaca gactgaaaag tgagtttctg gcaaatatga gccatgaaat
      961 aagaacgcct atgaatactg tgattggatt cgcggaaatt gtcagggaaa aaacatccaa
     1021 taatccggaa attcaggact atatggaagg cattcaaaag agcggaaaga ctctgatcag
     1081 tctaatcaat aatatattag atttatctaa aatagaatcg ggactgatgg aaatcagcaa
     1141 aggtccagtc aatctgaaat ctgctgtaag tgatacagtt cagatgttca gccttcaggc
     1201 aaaggaaaaa ggcattcatc tttatcagaa actggacaga gaccttccgg acggttttta
     1261 tctggatcag acaagaatga gacagattct gctgaatctg actggcaatg caatcaagtt
     1321 tactgataaa ggggaagttt gcatctccgt atcggctgag cagtctctgg aagataagaa
     1381 tgaatatact ctgaaaatgg aagtgacgga tacaggttct ggaattgcgg aaacggaaat
     1441 agaccgaata tttcatccgt tcattcagca gaaaggtcag gatatgagca gatacggcgg
     1501 ttctggattg ggcctttcca ttacaaaaaa gcttacagaa cttatgggag gctctctttt
     1561 tgtggagagt gtcatggggc agggttccaa tttcactgtc atcctgaaaa aagtcagaac
     1621 ggctgagctg gttaatatca gatcaaataa tcaattcttt gatattaatc atatcccatc
     1681 attgaacgga agaatacttc tcgtcgagga tgtggcaagc aacagaaagg tagtcaacgg
     1741 acttcttaga aagacaggca ttcagatact ggaagcggaa aacggacgga aggcagtcga
     1801 gattctggaa aaagaaaagg ttgatctgat tcttatggat atgcatatgc cggaaatggg
     1861 agggcaggaa gcatcaatcg taatcaaaag cagaccggaa tggtctgcta ttcctatcat
     1921 catactgacc gcagacgcga tgagtgataa tatcgaaagc tttaaggagt tttcagaagg
     1981 ttatctgact aagcctgtta caaaagaaga tctcattttc acattggcca agtttttggg
     2041 tgaataaaaa aactatgttc tgcaagttaa gactagtcag ctttaat
//
LOCUS       DAOQGJ010000630         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36163_length_2086_cov_2.825209, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000630 DAOQGJ010000000
VERSION     DAOQGJ010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36163_length_2086_cov_2.825209"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /gene="thiD"
                     /locus_tag="PK683_10365"
     CDS             <1..668
                     /gene="thiD"
                     /locus_tag="PK683_10365"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNH08896.1"
                     /translation="ITCITAQNPESVTGIQEISPEMIEKQMESVLSYFPVKAGKTGML
                     FSKEIMFAFISVYRKYSFPLVVDPVMVASSGARLLKETDIEFLKNEIIPAAALVTPNA
                     DEAEILLGEKIYLENLEESCRRIYDLYRVPVLLKGGHRPSEQAVTDILFDGKSVLRLE
                     SPLQRNISTHGTGCTYSASVCAELAKGETLQNSVRNAKKYLHETLEKGIYLNNTKSLN
                     HNV"
     gene            678..1970
                     /gene="aroA"
                     /locus_tag="PK683_10370"
     CDS             678..1970
                     /gene="aroA"
                     /locus_tag="PK683_10370"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNH08897.1"
                     /translation="MPESALKIKKDFKIKVPGDKSVSHRSVLFSALSSGKSRITNFLE
                     AEDPLNTLKSFAKMGLTYRKTGRFEYELDSPGKKGLKESSEVLDFGNAGTGIRLSAGL
                     LAGLRGIRSVLTGDESLRKRPMKRIIEPLTEMGADIRSENNDDKAPLIVSGKHLKDFF
                     HYKSILSSAQVKSCLMLGAVASETELEYEEEELSRDHTENMIRFLGGKIVHSDRLHFK
                     ITPPYDFQGTHFKVPGDISSASFFIVLGLLAESGSLVIEEVGLNPARTGIITVLKNMG
                     ADIKISNERSECGEIIGDIVVSPSKLKKTEIAKELIPSIIDEIPVLSIAGLFAEGGFS
                     ISNAEDLRAKESDRIHSMVLNLRKIGADVDEKKDGYSFGETKEIKPAVIETFHDHRIA
                     MSFMILSNITGTELKIDDESWIDTSFPGFQNLLRDIKS"
BASE COUNT          672 a          419 c          460 g          535 t
ORIGIN      
        1 tcatcacatg cattaccgca cagaacccag aaagcgtaac tggaatacag gaaatatctc
       61 ctgaaatgat tgaaaaacag atggaatcgg tgctctccta tttcccggta aaagcaggaa
      121 agacgggaat gcttttctca aaagaaatta tgtttgcatt catttcagta tacagaaagt
      181 acagtttccc tcttgtggta gatcctgtaa tggtggcaag cagcggtgca aggcttctta
      241 aagaaacgga tatagaattt ctgaaaaacg aaattatccc tgctgctgca ctcgtcactc
      301 cgaatgcaga tgaagcagaa atccttctgg gagaaaagat ttatctcgag aatctggaag
      361 aatcctgcag aagaatctat gatctttaca gggttccggt acttctgaaa ggcgggcaca
      421 gaccgtctga acaggcagtt actgacattc tgtttgacgg gaaatcagtg ctcagactgg
      481 aaagccctct tcagagaaat atcagcacac atggcactgg atgcacatat tctgcttcgg
      541 tctgtgccga actcgcaaaa ggcgagactc ttcagaactc agtacgaaat gcaaaaaagt
      601 atctccatga aaccctggaa aagggtatat atctgaacaa tactaaatca ttaaatcata
      661 atgtttaagg aacaactatg ccggaatctg cactgaaaat aaaaaaggac ttcaaaatca
      721 aagttccggg agacaaatcc gtctctcaca gatcagttct attttcggca ctctcatcag
      781 ggaaatccag aattacgaat tttctcgaag cagaggaccc tttgaacact ttaaaatcct
      841 ttgcaaaaat gggactgacc tatagaaaaa caggtcgttt tgaatatgag ctggacagcc
      901 cgggaaaaaa aggactgaag gaatcctctg aagttctgga ttttggaaat gcaggcacag
      961 gaataagact ctccgcagga ctgcttgccg gactacgggg aatcaggtcg gtactgacag
     1021 gtgatgagtc tctcaggaaa cggcccatga aacggatcat tgagcctctg actgaaatgg
     1081 gtgcagacat cagatcagaa aataatgatg acaaagcgcc tcttattgtc agtggaaagc
     1141 atttgaaaga tttttttcat tataaaagca tactttcgag tgctcaggtc aaatcctgtc
     1201 tgatgctcgg tgcagtagcc tcagaaactg aactggagta cgaagaagaa gagctttcga
     1261 gagatcatac tgaaaatatg attcggtttc tcggcggaaa gattgtgcat tcggacagac
     1321 ttcatttcaa aatcactcct ccttacgatt tccaaggaac tcatttcaaa gtgcctggag
     1381 acatatcctc tgcatccttt ttcattgtgc ttggacttct cgcagaaagc ggttcacttg
     1441 taattgaaga ggtaggtctg aatccggcca gaacagggat tattactgtt ctcaaaaata
     1501 tgggtgctga tattaaaata tccaatgaac gctcggaatg cggagaaatc atcggtgaca
     1561 tagtggtaag tcccagcaaa ctgaaaaaaa cggaaatagc aaaggaactg atcccttcca
     1621 tcatagatga aatccctgtt ctgtcaattg caggactatt tgccgaagga ggattttcca
     1681 tttcaaatgc ggaagatctg cgcgcaaagg aatctgacag aattcattcg atggttctga
     1741 atctcaggaa aatcggtgca gatgtggatg aaaaaaagga cggatacagt tttggagaga
     1801 cgaaagaaat aaaaccggca gtaattgaaa catttcatga ccacaggatt gcaatgagtt
     1861 ttatgattct ttcgaacatc acaggaactg aactgaagat agacgatgaa tcctggatag
     1921 atacctcctt tccaggattc cagaatctgc tcagagacat taaatcataa ttatattatg
     1981 atttctgcat ttccttgagc cggaaaattt ttccgagacc tgaagaataa gtatccctgt
     2041 tgtagagttc agcagtcttg agtcttcccg ttttctgaac ctgccc
//
LOCUS       DAOQGJ010000631         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36165_length_2086_cov_2.663712, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000631 DAOQGJ010000000
VERSION     DAOQGJ010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36165_length_2086_cov_2.663712"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="PK683_10375"
     CDS             <1..1036
                     /locus_tag="PK683_10375"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH08898.1"
                     /translation="DHGDYISDSCRKVVQSAALRFTSLMADNSTDHKNEKAMPLQDNI
                     LLTREVVDMAHPLGISVEGELGVLAGEEDEDTKSDHSTYTNPDELDQFLKGTGVLMLA
                     PTIGTMHGPNKGKPGQKVKLNVTLAHELLKIAEPMTQVGGDFYDFYKINDFTLGVIMA
                     DVAGHGVPAALVASMLKVAADLEARKSSNPAEILQGINRTMLKSGSNTFITAMFMFLN
                     LKTKKLTISKAGHPPIIIFSKKDSSIREYSFKGRLIGVYEDLKSSRHSIPVSSGERII
                     LYTDGVFEVPNEEDENYGEDRFREFVRAYGTLEPQEFHSLLVKELKEWMNRSDGTFAD
                     DMSIVTVDIL"
     gene            1118..1684
                     /locus_tag="PK683_10380"
     CDS             1118..1684
                     /locus_tag="PK683_10380"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HNH08899.1"
                     /translation="MENQENKSTQDIILTHVAFGMTAGAIPVPLLDIAAVSAVQLNMI
                     RQIAAHYNADFNSNLGKSLISAVLGSSIARAGASIVKAFPGIGTALGIGTQVVLSGAS
                     TYAVGKLFDSHFAGNGTLDSFNVEEMKQKFKELYEQGKKAAQDMYNESKDEDVYVKIE
                     KLNKLRESGAITESEFEEAKKNLLSKIS"
     gene            complement(1688..>2086)
                     /locus_tag="PK683_10385"
     CDS             complement(1688..>2086)
                     /locus_tag="PK683_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNH08900.1"
                     /translation="AFITFHAEATNFGVRLSEDIHSHGIGVGVAVNPGTPLSFVEHLL
                     PYVDMILIMTVDPGFYGQSFVKGGFEKIAKAREMIKGTNHIIQVDGGVNAHNIRRIYE
                     AGAHICVAGSAVFKTGDPNENAMLLKKHCE"
BASE COUNT          630 a          431 c          485 g          540 t
ORIGIN      
        1 ggatcacggt gattatatat ccgattcctg ccgcaaggtt gttcagagcg cagctctccg
       61 ttttacttcc ctgatggcag acaattcaac tgatcataaa aacgaaaaag cgatgcctct
      121 gcaggacaac attcttctga caagagaagt tgtggatatg gctcatcccc tgggaatttc
      181 tgtagaagga gaattgggag ttttggcagg agaagaagat gaggatacaa aatccgacca
      241 ctccacttac acaaacccgg atgaactgga tcagtttctt aaaggaacag gagttctgat
      301 gctggcaccg accattggaa caatgcacgg cccgaataaa ggaaaaccgg gacagaaagt
      361 aaaactgaat gtcacacttg cccatgaact tttgaaaatt gcagagccta tgactcaggt
      421 cggaggggat ttttacgatt tttacaagat aaatgacttc acactcggcg tcattatggc
      481 cgacgttgca ggacacggag ttcctgcggc tcttgtggca tccatgctca aggttgctgc
      541 cgatctggaa gcgagaaaat cttccaaccc ggctgaaatt cttcaaggga tcaacagaac
      601 aatgctgaaa agcggaagca atacattcat tactgcgatg tttatgttcc tcaatttgaa
      661 gacgaagaaa ctgactattt cgaaagcggg tcatcccccg atcattatct tttccaaaaa
      721 ggacagctct atccgtgaat acagtttcaa gggaagactg attggtgtat atgaagatct
      781 gaaaagcagc aggcattcca ttccagtcag ttccggagaa agaataattc tgtacacaga
      841 cggggttttt gaggttccga acgaggaaga cgagaattac ggtgaggaca gattcagaga
      901 atttgtcagg gcttacggaa ctcttgaacc tcaggaattt cattctctgc ttgtcaagga
      961 actcaaagag tggatgaaca gaagtgacgg aacttttgcg gacgatatga gcatcgtaac
     1021 tgtagacatc ctgtaaaatc tgcccgaaat caaagaaacg attgatttaa ttttgacctt
     1081 cctgtaagct agggaatact ttttctcagg agagaatatg gaaaatcagg aaaataaatc
     1141 aactcaggat atcattctga cacacgttgc gtttgggatg actgcaggcg caatcccggt
     1201 acctcttcta gacattgcag cagtgagtgc agtccagctg aatatgatcc gtcagattgc
     1261 cgcacattac aatgcagatt ttaacagcaa cttaggaaag tccctgattt ctgctgttct
     1321 gggttcatcc atcgcaaggg ccggcgcaag catagtgaag gctttccccg gcatcggaac
     1381 tgcgttagga ataggaaccc aggttgtgct ttcaggagca tctacatatg cagtgggaaa
     1441 actttttgac tctcattttg cagggaacgg tacactggac agttttaatg tagaagaaat
     1501 gaagcagaaa ttcaaggaac tgtatgaaca gggaaagaaa gcggctcagg atatgtacaa
     1561 tgaaagcaag gatgaagatg tttatgtaaa aattgaaaaa ttgaacaaac tccgagaaag
     1621 cggtgcaatt actgaatcgg agtttgaaga ggcaaagaaa aatcttctgt ctaagatttc
     1681 ctagagatca ttcacagtgc tttttcagga gcattgcgtt ttcattggga tcaccggttt
     1741 tgaacactgc tgatcccgca acgcagatat gtgctccggc ttcataaatt cttctgatat
     1801 tgtgcgcatt gactccgccg tccacctgaa tgatatgatt tgttcccttg atcatttctc
     1861 ttgcttttgc gattttttca aatccgccct ttacaaaact ctgtccgtaa aatccaggat
     1921 ctacagtcat aatgagaatc atatccacat acggcagaag gtgttcaaca aatgacagag
     1981 gagtgcccgg gttgaccgcc acaccgactc cgattccatg agaatgaata tcttctgaaa
     2041 gacggactcc gaaatttgtt gcttctgcat gaaatgttat gaatgc
//
LOCUS       DAOQGJ010000632         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36187_length_2085_cov_3.150739, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000632 DAOQGJ010000000
VERSION     DAOQGJ010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36187_length_2085_cov_3.150739"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1085..>2085
                     /locus_tag="PK683_10390"
     CDS             1085..>2085
                     /locus_tag="PK683_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HNH08901.1"
                     /translation="MRKIVKSIIWVMLFSFSACVNPSMQDKLRNSSAILLLLKKYPVP
                     EISYSSKSFAFFKGSAVSAVPTVKGKVKNCNIFPSLPAGMNFDKGTCTVSGTPQSLQS
                     EIQYTVTASEVSGKTFGTQIGISVNPIPIKLNYPDSPYSFFKNGPVTAVSPVLTGTAV
                     SYSISPSLPAGMVFDTQTGQISGTPTVYSPAADYTVTVTGEKETQTSVISLKVDFMRP
                     WPVLQPILMAQAGLGVSAAETASTIGTLAGPGTNWYGGVLAPNGIIYGIPRLHTSVLR
                     IDPSSNTVSTFGTFAGGVQKWWGGVLAPNGKIYGIPNSATNVLQIDPDTDTAGTFGSA
                     "
BASE COUNT          595 a          419 c          403 g          668 t
ORIGIN      
        1 aaattgtttc atttcaatga cggccggaaa atgaaaattg cggtagtttt caagtttttt
       61 acttaaatct ggatgaattt tcatgaactg tccttgtact ttgcaatcgg tctaattttc
      121 atcagataaa gcttctgtca atcggaataa atttttcaga agtatatgct catattcaaa
      181 agtatttgcg aatttcaatc ttgctgagcc tgtaaaatca aggtttctga cgaaaaagca
      241 caattacttt cggcaatcag cataacttgc agatatgtca gtattctata aaataattta
      301 ttatttatcc atatttccgc tgattagatt atgtctctta tggaaaatta ttttcggaaa
      361 aattaccgta aagcaggcat tagtactgat ttcagatgct caatttatag ggtcagacgg
      421 gaaatgctgt tttcgaatct gtaaaaagtg atctgaactg tgctgtattt cctccgtcag
      481 ctttcctctg agactgccct gcagtccaaa acaggagagc atagtcggta ctgcctcttg
      541 agcggatcag atctcaggtt ctgcttaccc ggacttcagc agaaccaagc cgatacgttt
      601 ttgctgctgt cgcagagaat atacaatgtc gactgattat ttttcatcaa tgaagagata
      661 tttcattata aagaattatt ttcataaaaa aattataaag tatttaaatt ataagaaagt
      721 ttaaagaaat cccggatctg gtccgatgtt tttttaaatg atcgattgtc tcaatttcag
      781 tatggaatat tttcaacccc ctttgatttt ttcatcattt ctgcacgaca cctgtccgaa
      841 ttttttgtac atccttgtaa agctgcatta attgcatttg cagtcattta ctcaattcag
      901 aagtccaaaa caggcagata aaggaaaatt cctgtttttt aaacaaatat cattatttta
      961 aaaaagtaca gtccttttaa aattcccctt cagcaaaaaa aaagtgcagg atattttttt
     1021 ttgttgaatt attccacggc tcttactgtc attttactaa acgcatatcc gactggagta
     1081 tggaatgaga aaaattgtaa aaagtataat ttgggttatg ctcttttctt tctcagcctg
     1141 tgtgaatcct tctatgcagg ataaacttcg gaattcgtct gcaattctgc ttttactgaa
     1201 aaaataccct gttccggaaa tttcctattc gagtaagtct tttgcattct tcaaaggctc
     1261 tgcagtgtct gctgttccga cagtcaaagg aaaagttaaa aactgtaata tttttcccag
     1321 tcttcctgcc ggtatgaatt ttgacaaagg gacatgtact gtttccggca caccgcagag
     1381 tctgcagtct gaaatccagt atacggtaac cgcttccgaa gtttccggga aaacatttgg
     1441 aactcagatt gggatatctg tcaaccctat tcccattaaa ctgaattatc cagattctcc
     1501 ctattctttt ttcaaaaacg gtccagtgac agctgtttct ccagttctga ccggcaccgc
     1561 agtgtcttat tcaatatcac cctctctgcc tgccggaatg gtcttcgaca cgcagaccgg
     1621 tcagatttcg ggaactccga ctgtctattc ccccgccgca gattatacag tcacagtgac
     1681 aggagaaaag gaaacgcaga catctgtgat ttccttaaaa gtggatttta tgcggccctg
     1741 gccggtgctt cagccgattc ttatggcgca ggcaggttta ggcgtaagtg cagcagaaac
     1801 agcctctacg attggaactc tggctgggcc tggaaccaat tggtatggag gagtgcttgc
     1861 tccgaatgga ataatttacg gtattcccag actgcataca agtgttttgc gaattgatcc
     1921 tagttcaaat acagtctcta cttttggaac ctttgccggc ggtgttcaaa aatggtgggg
     1981 aggagtgctg gcaccgaatg gaaaaattta tggtattccg aacagtgcaa cgaacgtttt
     2041 acagatcgat cctgatacag atacagccgg cacctttggc tctgc
//
LOCUS       DAOQGJ010000633         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36205_length_2084_cov_7.642681, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000633 DAOQGJ010000000
VERSION     DAOQGJ010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36205_length_2084_cov_7.642681"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PK683_10395"
     CDS             complement(<1..385)
                     /locus_tag="PK683_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08902.1"
                     /translation="MTIDNTGAVSIPGTLSKGGGTFDITHPDPSMPKGSRLRHSFVES
                     PTAGDNIYRWQIKVTKENRKFFIKLPKYWTHLNENPMVWVSSAEHPAASFGYVDTKQE
                     KVIIQADRTGTYNVLLLGTRKDSLAA"
     gene            556..2025
                     /locus_tag="PK683_10400"
     CDS             556..2025
                     /locus_tag="PK683_10400"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HNH08903.1"
                     /translation="MNVGEIKQAFRNGWNGIYSIIKKLLSQIKNLQSQVSSLETKNQE
                     LEERLKKLIGEKSKSSRNSSRPPSADGLKKKTKSLRKKSERKSGGQKGHEGSTLEMTD
                     APDEIIKIKLHHCKGCGRKISARNKWTVSRQIIDLDIKKKVVEYRAECAVCSACGRES
                     SAAFPDFCSKKVQYSPKVRALSVYLNKYQLIPLKRVAEIFSDLHGIKISEGSIVNFSA
                     QLFDELEIFESECRDALLQSELIHADESGGRCEKKLQWFQVVSNKVITFIAFHHKRGT
                     EAMNDIGILPNFKGKTVHDFYRSYFTFDVQHILCNAHLLRELIFEYEQNLQKWAGRMI
                     QLLVEIKETVDREKEKGTRNSLSPYMIKKYEAEFESILKSGFRENPYQCREQGKRGRA
                     KQTSSRNLLDRLKEHRMSYLGFMNDFNVPFDNNLAERDIRMLKLYMKISGCFRSLSGA
                     AYFCRIRSYISTARKNNLNTFDSIFNAFLGNPFSPKFAE"
BASE COUNT          657 a          413 c          471 g          543 t
ORIGIN      
        1 cagccgcaag agaatccttt cttgtaccaa gaagcaatac attgtatgtt cctgtcctgt
       61 ctgcctgaat aatgactttt tcctgttttg tatccacata accgaaggaa gcagcaggat
      121 gttctgctga ggaaacccag accatcggat tttcattcaa gtgtgtccaa tacttcggaa
      181 gtttgatgaa gaattttctg ttttcctttg tcaccttaat ctgccatctg tatatattgt
      241 ctccggcagt aggggattcc acaaaggaat gcctgagcct cgaaccctta ggcatagacg
      301 gatctggatg agttatatca aatgtaccgc ctcccttgga gagagttcct ggaatgctga
      361 ctgccccagt gttatcaatg gtcattctgg tagcatagga agtggatcca tttggtgtgg
      421 tataaaagac catccgactt ccatgactgc ttgcattcca gttttctgtc gcatcgctat
      481 ttatgtatgt agaatgtaac tactcagcga aaaaaagaat tgtacaaaag aagagttctc
      541 tgacaatgac cggaagtgaa cgtaggggaa ataaagcagg ctttcaggaa cgggtggaat
      601 gggatatatt ccataattaa aaaactcctg tcgcagataa aaaatcttca gagccaagtt
      661 tcttcattgg aaacaaagaa tcaggagctg gaagagagac tgaagaaact gattggagaa
      721 aagtccaaaa gcagtcgcaa cagcagcaga cctccgtcag cagacgggct taagaagaag
      781 actaaaagtc tgcggaagaa atcagagagg aaatccggcg gtcagaaagg ccatgaggga
      841 agcactcttg aaatgacaga tgctcccgac gagattataa aaattaaact gcatcactgc
      901 aaaggctgcg gcaggaaaat ttcagccagg aacaaatgga ctgtcagccg gcagataata
      961 gatctcgata taaaaaagaa agttgttgaa taccgtgcag aatgtgccgt atgctctgcc
     1021 tgcggcaggg aatcatctgc cgctttccct gacttctgtt caaagaaagt gcagtacagt
     1081 ccgaaggtaa gagcattgag cgtctatctg aataagtatc agctcattcc tctgaaaagg
     1141 gttgcggaga ttttctccga cctgcacggc attaaaattt cagaaggcag catcgtaaat
     1201 ttcagcgcgc agctttttga tgaactggaa atttttgaga gtgagtgcag agatgcactg
     1261 ctgcagtcgg agctgatcca tgccgacgag tcaggcggca gatgcgaaaa gaaactgcag
     1321 tggtttcaag tcgtgtcgaa caaagtgatt acattcattg cgtttcatca taagcgcgga
     1381 acggaagcaa tgaatgacat cggaattctt ccgaacttca aaggaaagac agtccacgat
     1441 ttctacagat catattttac attcgacgtt cagcatattc tgtgcaatgc ccatcttctg
     1501 cgggaactga ttttcgaata tgaacagaac ctgcagaaat gggctggaag aatgattcag
     1561 ctgcttgttg aaatcaagga gacagtcgac agagaaaaag aaaaagggac aagaaattct
     1621 ctttcgcctt atatgatcaa aaaatatgaa gctgaatttg aaagcatatt gaaatccggc
     1681 ttcagagaga atccttatca gtgcagagaa caaggaaaaa gaggaagagc aaagcagaca
     1741 tcgtctcgaa atctcctgga cagactcaag gaacacagaa tgagttatct gggttttatg
     1801 aatgatttca atgttccttt tgacaataac cttgcagaaa gggatattcg gatgctgaaa
     1861 ttatatatga aaatctccgg atgcttccgt tccctttccg gcgctgctta cttctgcaga
     1921 atcagaagct atatctcaac tgccagaaaa aataatttaa atacttttga ctccattttt
     1981 aatgctttcc ttgggaaccc attctctcca aaattcgctg agtagttaca aaaattttag
     2041 ataatcattt ttcttattga atctcgctgg actgatactg aaat
//
LOCUS       DAOQGJ010000634         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36232_length_2083_cov_3.420611, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000634 DAOQGJ010000000
VERSION     DAOQGJ010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36232_length_2083_cov_3.420611"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1377
                     /locus_tag="PK683_10405"
     CDS             235..1377
                     /locus_tag="PK683_10405"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNH08904.1"
                     /translation="MKTILITLSLILTSCIRLEPSVLDTSKNPAALIASFLLGSGTGA
                     AGGTTGISYTGQTQCFYATGTTWNLDANCTQTYTVGNFNFPYGQDAHYLRKPYSRSFT
                     GPTLVGTSDYITTDKITGLVWKTCTEGLSGSNCGTGTASAYTWTAADTACTALNSGAG
                     YAGRTDWRLPTIKELRTLPDYGKNPASDASYFPKIAANNYWASTVRAALTTNAYYMVM
                     STGGELFGLKTTSTYNALCVSGSMSGTQTLTDNGDGTVSESTTGLLWQKCLAGQNALD
                     CSGTANSVYWSDAITYCENLNFAGRTNWRLPDVNELKNLIVDTQTGPYINLTYFPNLG
                     NSDCWSSTSAFSGPGSAWTVDFSNGFARTRGKTFVVTFGLRCVSGP"
     gene            1387..1683
                     /locus_tag="PK683_10410"
     CDS             1387..1683
                     /locus_tag="PK683_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08905.1"
                     /translation="MKSFYLLLSILLVSCIRLEPSVLDTSKNPGALLASLLLASVTGT
                     GTASGTGTLAASSFITLGSPFWVGVNSSANLLYVTNYNSNTVTFFNAATGAYAF"
     gene            1737..>2083
                     /locus_tag="PK683_10415"
     CDS             1737..>2083
                     /locus_tag="PK683_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08906.1"
                     /translation="MNQRLNFHSDIKPRLFFFFCVVFLAAAFAPMQAEEILLKTGAVI
                     LGRVSKINADAVFVTTAKGNQSIPKANIIRIQNTPFTAEQKAKALEAQQKKKAAIALE
                     QDQIRNIQEAKER"
BASE COUNT          577 a          472 c          484 g          550 t
ORIGIN      
        1 ctgtactgat tgtgagtctt cttctctgtt caaggacagg aaagaggaat ggaacaaata
       61 ccagtacacc gactgaaaca gtgctcgcgg aagccttcgg aaagactaat actgaagatg
      121 tacactgtgt ttccgggggt tgacagtgaa tcccatttta aatctgctgt atacagcaaa
      181 ctatggttca aaaaagataa cagttttcaa cgccactaca ggagcctaag cattttgaag
      241 acaattctaa tcacattatc actgatactc acttcctgca tacgtctcga gcccagcgtt
      301 ctcgatacat caaagaatcc tgcggcactg attgcgagtt ttcttctggg ttctggcaca
      361 ggggctgccg gaggaaccac tggcatctct tacacaggtc agacgcagtg tttttatgcc
      421 accgggacaa cttggaattt agatgcaaac tgcactcaga cgtacactgt gggaaatttt
      481 aattttcctt atggtcagga tgcacattat ctgagaaaac cgtattcacg cagttttaca
      541 ggaccgacgc tcgtaggaac atctgattat attaccacag ataaaataac aggtctcgtg
      601 tggaaaacct gtacggaggg cttgagcggt tcaaactgcg gaacgggaac tgcctctgct
      661 tatacttgga ctgctgcgga tactgcctgt actgcactga acagcggagc cggttatgca
      721 ggcagaacgg actggcgtct tccgacaatt aaagaactcc gaaccctgcc tgattatggg
      781 aaaaatcctg cttctgatgc atcctatttt cccaaaattg ctgccaacaa ctattgggct
      841 tctactgtaa gagctgcttt gaccacgaat gcatattaca tggtgatgtc taccggaggt
      901 gagcttttcg gtctgaaaac aacatccact tacaacgcac tctgtgtttc cggaagtatg
      961 tctggcacac agaccctgac agacaacgga gacggaactg tttcagaaag tacgacagga
     1021 cttttgtggc agaaatgcct ggctgggcag aatgcattgg actgttccgg aactgcgaat
     1081 tctgtatact ggtcggatgc gattacctac tgtgagaatc tgaattttgc cggcagaaca
     1141 aactggcgcc tgccggatgt caatgaactg aagaatctga ttgtagacac tcagactggt
     1201 ccttatatca acctgacata ctttcccaac ctgggaaatt ccgactgctg gtcttctacc
     1261 tcagcttttt ccggtcccgg aagtgcatgg actgtggatt tctccaacgg ttttgcacgg
     1321 acacgcggga aaacatttgt ggtcactttt ggcttacgct gtgtttcggg gccgtaagga
     1381 ggcgctatga aatcatttta tttgttatta tcgattttat tagtatcctg cattcgtctc
     1441 gaaccgagcg ttcttgatac atcgaagaat ccgggagctt tgctggcgag tctgcttctg
     1501 gcctcagtga ctggaacagg gactgcgagc ggaacgggaa cgctggcggc ttccagtttc
     1561 attaccctag gcagtccatt ttgggttgga gtgaattctt ctgcgaatct gctctacgta
     1621 acaaactaca attcaaatac agttactttc ttcaacgctg caacaggagc gtatgcattt
     1681 tgaaaatccc gtatatagaa cgaattaaat acaaattaat taaggaaaaa tataatatga
     1741 accaaagatt aaactttcac tcagacatca agccgcgtct attcttcttt ttctgtgttg
     1801 tctttctggc tgcggctttc gcaccgatgc aggcagagga gattctcctc aaaaccggtg
     1861 ctgttattct gggacgtgtt tcaaagatca atgcagacgc cgtgtttgtg acgactgcca
     1921 aagggaatca gagcataccc aaggcaaaca tcatcagaat tcagaatact ccatttactg
     1981 ctgaacagaa ggcaaaggca ctcgaggcac agcaaaaaaa gaaagctgca atcgcattgg
     2041 aacaggatca gattcgaaat attcaggaag cgaaagaacg gaa
//
LOCUS       DAOQGJ010000635         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36245_length_2083_cov_2.596154, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000635 DAOQGJ010000000
VERSION     DAOQGJ010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36245_length_2083_cov_2.596154"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1685)
                     /locus_tag="PK683_10420"
     CDS             complement(105..1685)
                     /locus_tag="PK683_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH08907.1"
                     /translation="MSTKNNLSKKLQIEIKGETTSNYLRFFLVSVFTLGTIMAFFLKG
                     DVYTIIEYYCVGIFLYAFSIPISLYFLRTDQYSPKVKYITSAFEYVGFAIVQMGYFSA
                     EMIYKANGVLSPIMFSVFFLLVGGASLRFSKRFTGIVSASYVVLYLCLAVGLKTAFPE
                     LAVLGSKGPPKLTVITVSVSALFLSAMGITLTIATGYVQNVLYESQEAEDRARENYEN
                     AELLIYKISYVTAELNSIIPAIDGSAKVTEEKGRDLKNSMEISLESIRKLSSSISDIA
                     SKTSEQFQLGEQNILSMNELKTTMSELNKESKRVSKKGVLAIENAQKGEQKLEEINEE
                     IENLLGNSRKVGEIVRLINDISKRTNLLALNAAIEAARAGEEGKGFTVVSDEVRKLAE
                     TSGRNAAEISALIQQMKTDTENSAESLRDIVGIIRIIIMGIREIVEGVQMISESLEQQ
                     SGVSTEISMSLERIQEKIKNVRDNTSLQNAESKTIRSAVEAITQNLKDLTDHAESLHT
                     ASKSLGAKSRELQEAAKR"
     gene            complement(1669..>2083)
                     /locus_tag="PK683_10425"
     CDS             complement(1669..>2083)
                     /locus_tag="PK683_10425"
                     /inference="COORDINATES: protein motif:HMM:NF018504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08908.1"
                     /translation="WAVLTRLEVPKKANLTLLQKLKLYNGKSLPGFTEDNVRELRKEA
                     GREGMEGISPRYVQDKISNALVNTRDGNASCLNPFMVLNELESGLKNHSLISSEEKNQ
                     PIVDGMLFFRKLLKSKSTEEDRAARKILKKYVNEK"
BASE COUNT          554 a          466 c          408 g          655 t
ORIGIN      
        1 tgatggactt taccggaact tatgatgaat atctttccaa ggaaggaaac gatttcttca
       61 aaagacagaa cagctcagcg gttcttgcag gttcaaagta aaaattatct cttcgcagct
      121 tcctgaagtt cccttgattt tgcaccgaga gacttggaag ctgtatgaag agactctgca
      181 tgatctgtaa gatcctttaa attctgcgta atagcttcca ctgcacttct gatcgtcttg
      241 gattctgcat tctgaagaga ggtattgtct cttacattct tgatcttttc ctgtatgcgt
      301 tccaggctca tactgatttc ggtggaaaca ccagactgct gctccaaaga ttctgaaatc
      361 atctgaacac cttctacaat ctcgcgtatt cccataataa tgatacggat aattcccaca
      421 atgtctctca ggctttctgc actgttctca gtatctgtct tcatctgctg aataagtgcc
      481 gatatctcgg ccgcatttct tccggaagtt tctgcgagtt ttctcacctc atccgacacc
      541 acagtgaatc ctttgccctc ctcacctgct cttgccgctt ctatagctgc attgagtgcc
      601 agaagatttg ttctcttgga aatatcattg atgagcctta caatctctcc tacctttctg
      661 gaatttccaa gaaggttttc aatctcttca ttgatctctt cgagtttctg ctctcctttc
      721 tgagcattct caattgcaag gactcctttt ttggaaactc ttttggactc cttgttcagc
      781 tcgctcatag tagttttcag ttcattcatg gaaagaatgt tctgttctcc cagctgaaac
      841 tgctcggatg tcttggaagc aatatccgat atggaggaag aaagttttcg gatggattcc
      901 agtgatatct ccattgagtt tttaagatct ctgccctttt cttcagtgac cttggctgat
      961 ccgtcaattg caggaataat tgaattcagt tcagcggtca cataactgat tttataaatc
     1021 agcagttctg cattttcata gttttccctg gccctgtcct cagcctcctg ggattcatag
     1081 agtacattct gaacataacc tgtcgcaatg gtaagtgtaa tccccatcgc agaaagaaaa
     1141 agtgcggaaa cggaaacagt gatgactgtg agtttaggag gaccttttga acccagaact
     1201 gcgagttcag ggaatgctgt tttaaggccg actgccagac agagatacaa tacaacatag
     1261 gatgcggata cgattccggt aaaacgtttt gaaaaacgga gagaggctcc ccccaccagc
     1321 agaaaaaata cagaaaacat gatcggactc aggactccgt tagctttgta tatcatttct
     1381 gctgaaaaat atcccatctg aacaatcgca aatccgacat actcaaatgc agatgtgata
     1441 tactttacct tgggtgaata ctgatctgtt ctcaggaaat ataatgatat cggaatggaa
     1501 aatgcataga gaaatattcc gacgcagtaa tattctataa tggtataaac atcacctttc
     1561 agaaagaagg ccataatcgt tcccagtgta aaaacggaaa ctagaaaaaa acgcagataa
     1621 ttggaagttg tttctccctt gatctcgatc tgcagtttct tggacagatt atttttcgtt
     1681 gacatacttc tttaatattt tcctggctgc tctgtcttct tctgtcgact tgcttttcag
     1741 aagcttcctg aaaaaaagca taccgtctac aatgggctga ttcttttctt ctgaagaaat
     1801 cagagagtgg tttttaagac ctgattccaa ttcgttcaga accatgaagg ggttcaagca
     1861 acttgcgttg ccgtctcggg tattgaccaa cgcgttcgag attttatctt gaacgtagcg
     1921 tggagaaata ccttccatgc cttctcgacc cgcttctttt cgaagttcac ggacgttgtc
     1981 ttccgtaaac ccgggcaaac tttttccgtt gtaaagtttc aatttttgta aaagagttag
     2041 gtttgctttt ttcggaactt ccaaacgcgt taacacggcc cac
//
LOCUS       DAOQGJ010000636         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36277_length_2082_cov_2.820424, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000636 DAOQGJ010000000
VERSION     DAOQGJ010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36277_length_2082_cov_2.820424"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..810)
                     /locus_tag="PK683_10430"
     CDS             complement(100..810)
                     /locus_tag="PK683_10430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH08909.1"
                     /translation="MTELSAIVTGAGRGIGLAVSQMLLQEGYTVHGIARSLPDNLQNE
                     KFLFHKMDLSDIDSLEKNLSPLKKLCSNLKVLVLNAGIGLFGLHEELPIKKLNEMMLL
                     NFVSPVITARIFLRLLKNNQGTMVTVSSASSLRGSALAAAYSGTKAGLSQFSRSVFDE
                     SRKSGLRVVNIVPDIVSTDFYRNNWFEPESENDSFLYPEEVAEAVRTCIGVRPEMNIS
                     EIHLEPRLKRIRKKKFLF"
     gene            complement(803..1627)
                     /locus_tag="PK683_10435"
     CDS             complement(803..1627)
                     /locus_tag="PK683_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA photolyase"
                     /protein_id="HNH08910.1"
                     /translation="MRLILAEKKDQFYYPGSQIADNLKHPRFYYNSLILNCMYNCEYC
                     YLQGMYNSGHIVIFVNLEDFFRETDVLLSSGEETYLCISYDTDLLAFESLVPYASEWI
                     RFAEKRPNLLLEIRTKSAGFSQIEHIEPIPNVILAWTLSPAAAARRYEKKTPTLKRRM
                     ESLQAAVKKGWRTRICFDPILRFPDWKKEYSELIDTVMEVPELRNVNDITVGVFRINA
                     KYLKNIKKMRTDSELIYYPFKRNGDIASYKKEYEQEMLDFFISHLEKYYRSEKIYD"
     gene            complement(1786..>2082)
                     /locus_tag="PK683_10440"
     CDS             complement(1786..>2082)
                     /locus_tag="PK683_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08911.1"
                     /translation="NAYLSSLNSKKDRDSLIYAYYGSLDSSGSSYSGSDSTLLTGCSS
                     LGTKYAPSVCIVPQISAISTGNSFAKTQDVGKLAHTDLNLRSGTVRNYLLLDLP"
BASE COUNT          602 a          441 c          400 g          639 t
ORIGIN      
        1 aaatgctgtc tatttgccgc ttaggtaaaa tgtttacgcc agtgtgactt tctcagtcca
       61 ctggcctcca aattgtttct tacatatgct caattttttt taaaataaaa acttcttctt
      121 tctgatcctc ttcagtctag gctcaagatg aatttccgaa atattcattt ccggtctgac
      181 gccaatgcat gttcttacag cctctgccac ttcctccgga tagagaaaag aatcgttttc
      241 ggattcgggc tcaaaccagt tgttcctgta aaaatctgtg gatacaatat caggcacgat
      301 gtttacaact cggagaccgg attttctcga ctcatcaaag acggatcggg aaaactgaga
      361 gagtcctgcc tttgttccgg aataagcagc tgcgagagca gaaccgcgaa gggaagatgc
      421 agaagaaacg gtcaccattg ttccctgatt attcttcaga aggcgaagaa atattctggc
      481 agtaatgacc ggggaaacaa aattcaggag catcatttca ttcagttttt ttattggaag
      541 ctcttcgtgc agaccgaaaa gcccaattcc cgcattcaat acaagcacct tcagattgga
      601 acagagtttc ttcaaaggag aaagattttt ctccagtgaa tcaatgtcag acagatccat
      661 tttatgaaac agaaattttt cattctgcag attgtcagga agagatctgg caatcccgtg
      721 aactgtatat ccttcctgaa gaagcatctg agatacagca agtccgattc ctctgccggc
      781 ccctgtcact atcgcagaca gttcagtcat agattttttc cgatctgtaa tatttttcca
      841 gatgggatat aaaaaaatcc agcatctcct gttcgtattc tttcttatag gaagcaatat
      901 ctccgtttct tttaaacgga taataaatca gttccgagtc ggttctcatt ttctttatgt
      961 tcttcagata ttttgcattg attctaaaaa cacctacagt aatatcattt acatttcgaa
     1021 gttcaggaac ttccattact gtatcaatca gctctgaata ctcttttttc cagtcgggga
     1081 atctgaggat aggatcaaaa cagattcttg ttctccatcc tttttttaca gctgcctgaa
     1141 gactttccat tcttcttttc agagtaggag tcttcttctc atatcttctc gcagcggcag
     1201 caggagaaag agtccatgca agaattacat tcggaatggg ttcaatgtgc tctatctgtg
     1261 aaaaacccgc gcttttcgtg cggatctcca gaaggagatt cggccttttt tccgcaaacc
     1321 gtatccattc cgaagcatat ggaacaagtg attcaaatgc aagcagatca gtatcatacg
     1381 aaatgcagag ataagtttcc tcgcccgaac ttaaaagaac atccgtttct ctgaaaaaat
     1441 cttcgaggtt tacaaagatg acaatgtgac cggaattgta cattccctgc agatagcagt
     1501 attcacagtt gtacatacag tttaatataa gtgaattata ataaaatctg ggatgtttca
     1561 gattgtctgc aatctgggaa cctggataat aaaactgatc ctttttctcc gcaagaatca
     1621 gtctcataca ggatttctgt gcttgaaagt tttgattact tctgttgaaa atattcttgt
     1681 aatcagcaat cggaattact gcagcctgac tctgtttcct gatgatctgc tttgttcttt
     1741 ccaaatgcag aactttttct tctgcatata tatgagtaaa ccgaatcaag gaagatcaag
     1801 aagcagataa tttctgactg tgccggagcg aagattcaaa tccgtatgag ccagtttccc
     1861 tacatcctgt gtttttgcaa aactgtttcc ggtgctgatt gcacttatct gaggaacgat
     1921 gcagacagaa ggagcatatt tagtgccgag agaactgcat ccagtcaaaa gagtgctgtc
     1981 agaacccgaa taactgcttc cggaactgtc caatgaaccg taataggcat atatcagtga
     2041 atctctgtcc tttttggaat tcagagaact caaataagca tt
//
LOCUS       DAOQGJ010000637         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36316_length_2081_cov_2.355874, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000637 DAOQGJ010000000
VERSION     DAOQGJ010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36316_length_2081_cov_2.355874"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PK683_10445"
     CDS             complement(<1..261)
                     /locus_tag="PK683_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08912.1"
                     /translation="MNRSTLSIFFSAACILVSLHCGNERSRYFKNSEANIQLSLIFSI
                     KNQTCGGSRSVDTPFFGNIRKEDIHSCLRILMMVPCTTWVNTA"
     gene            467..>2081
                     /locus_tag="PK683_10450"
     CDS             467..>2081
                     /locus_tag="PK683_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH08913.1"
                     /translation="MEDELKIFLSESYETLNSLDKELILLSKNPGGATQSLHTIHANY
                     RTFLESSKFLRFQKLESLTHIGERLVNQVIESELDISSEMITTILKLNTTIREIIFTI
                     DETGKEPTHINSNIISDVEDVIKKSEQESLTEVDDGGSSELLLAGAPATGSAENDIMD
                     RLTNILVELIHTRVTVEKFQSELRNLKFYQAVSRMTSLINDMYNQVRQAKNQPIGSLM
                     QNLDKIVKDIAKSSGKSIALRVIGKDREVDSRQIEGIRICLIQLLKNSIEHGIEDPDE
                     RIARDKNPDGEITIECTVRGELFHIIVSDDGDGLDPDKIRKRLVDRNLLLTDEAEQIP
                     DYDIVHYIFKDNFSGLGEHGNFIGGRPTGFDIVKVKLESMGGRVYLQDSIRGKGMEVH
                     MTFPVINSIVPIISVAFGRERYAIAKAHLNEVLQIDSETLIENITVIHGYSVFHWKAK
                     KYPLLFMKQILKAEDNPHEENPIVNLIILESRGMKVALVGDKVQDMEEAVVRPLSTQI
                     NGIYLFSGMTILQDEKPALILDVGEIVRKN"
BASE COUNT          631 a          412 c          491 g          547 t
ORIGIN      
        1 ggcggtgttg acccaggtcg tgcaggggac catcatcagt attctgaggc agctgtggat
       61 gtcctctttt ctgatatttc cgaaaaaggg agtgtccact gatcggctcc ctccgcaggt
      121 ctggtttttg attgaaaaaa tcagcgagag ctgtatattc gcctccgagt ttttaaaata
      181 cctgcttctt tcattcccgc agtgcaggct gacaaggatg caggcagcgg aaaaaaatat
      241 gctgagtgtc gatctgttca aactttcccg tatcctgcag tgcagtctta gatgccaaac
      301 tgccgtcttt caagaaaata gaatttgttc tatttgcaac aaaatttcaa aaaaatgatg
      361 cgctcccgtt ccaaaaatca gtgtttatga gtgacgtaaa aatccttttt ttttcctgtg
      421 aaactgccga agtttaaaaa agcagctaaa ttcctggaag aagattttgg aagacgaatt
      481 aaagatattt ttatccgaaa gttatgagac gttgaactct ctagataaag agctcattct
      541 gctcagcaag aatccagggg gagcaactca gtctctgcat acgatacatg caaattacag
      601 aacatttctg gaatcttcca agttcctgcg gtttcagaaa ctggagtccc tgactcatat
      661 tggagagagg ctggtcaatc aggtgattga gtctgaactg gacatttcca gtgaaatgat
      721 tactactatc ctgaaactca atactacgat acgtgaaatc atttttacga ttgatgagac
      781 tggaaaagag cctactcata tcaattccaa tatcatcagt gatgtggaag atgtcataaa
      841 aaaaagcgag caggaatccc tgacggaagt ggatgacgga ggttcctcgg aacttcttct
      901 tgctggagct ccggcgacgg gaagtgcaga aaacgacatc atggacaggc tcacaaatat
      961 tctcgtggaa ctgattcata ccagagtcac tgtagaaaaa ttccagtctg agctccgtaa
     1021 tctgaaattt tatcaggccg tttccaggat gacctcactc atcaatgata tgtacaatca
     1081 ggtgcgtcag gcaaaaaatc agcccatcgg aagcctgatg cagaatctgg acaagattgt
     1141 aaaagacatt gcgaaaagtt caggaaagtc cattgcgctg cgtgtcatcg gaaaggacag
     1201 agaggtggac agcaggcaga ttgaaggaat cagaatctgt ctgattcagc tcttgaaaaa
     1261 ttccattgaa catggaattg aagatccgga tgaaaggatt gcccgcgaca aaaatcctga
     1321 cggggaaatt acgattgagt gtactgttcg aggagaactt tttcatatta tagtcagtga
     1381 tgacggggac gggctcgatc cggacaagat caggaagaga ctggtggaca gaaacctgct
     1441 tctgactgac gaggcagaac agattcctga ttacgatatc gttcattata ttttcaagga
     1501 caatttttcc ggacttggcg agcacggaaa tttcatcggt ggaagaccaa ccggttttga
     1561 tattgtcaaa gtgaaactgg aaagtatggg cggaagagtc tatcttcagg attctattcg
     1621 aggaaagggg atggaagtcc atatgacctt tccagtgatc aattccattg ttccgatcat
     1681 atccgtagca ttcggtcggg aaaggtatgc aattgcaaaa gcccatctga atgaggtgct
     1741 tcagattgat tcagagacac tcattgaaaa tattactgtc attcacggtt attccgtatt
     1801 tcactggaaa gccaaaaaat atcctcttct gtttatgaag cagatactca aggcggaaga
     1861 caatcctcac gaagaaaatc cgattgtaaa tctcattatc ctcgaaagca ggggaatgaa
     1921 ggtggcgctt gtgggagaca aggttcagga tatggaagaa gcagttgtac gtcccctaag
     1981 cacccagatc aacggaattt atctgttttc cggaatgacc attcttcagg atgaaaagcc
     2041 tgcactgatt ctggacgttg gtgaaattgt cagaaagaac c
//
LOCUS       DAOQGJ010000638         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36335_length_2080_cov_3.316543, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000638 DAOQGJ010000000
VERSION     DAOQGJ010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36335_length_2080_cov_3.316543"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PK683_10455"
     CDS             complement(<1..303)
                     /locus_tag="PK683_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNH08914.1"
                     /translation="MLLLLFSIGCSAFLYAEIGNCADCHMMEKQFSVWMKDGHRTAAN
                     CIDCYHADEKEMKGSVEQIQDRNYELRNPAMKSLTDLIFDIKTIKDKGADISRVQSF"
     gene            690..1550
                     /locus_tag="PK683_10460"
     CDS             690..1550
                     /locus_tag="PK683_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08915.1"
                     /translation="MYNRFDERLSEEAEKFYRSGTGNKLMQKTGKVGKRMKSDPGADD
                     DLHPLLNAAGIWMEEYARRKKWNGDELSETYIAITEKILQILFRFKKNGYSNFPSYLG
                     KYIKNCRMNIMRRDFERDNPALFELWDEEGNFRRALTAEEPLKKNQNLRNALLALNPL
                     NRMVISMRYNLSFMKSDADTFLKLMNESDRTEMMCRKEKLYQWEQKFLERLTGLTKRI
                     LERKDNERKLLSERKKRLLKKLMRPRELFSIAELGKIFGMPESRIARVCKVSISQIRE
                     EYEKSLKSSD"
     gene            complement(1779..>2080)
                     /locus_tag="PK683_10465"
     CDS             complement(1779..>2080)
                     /locus_tag="PK683_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNH08916.1"
                     /translation="LFCSFSCLVLFSLSPFLYSSFSSPGLEFIWNHPSNPFLALSFRE
                     IEILKLFLSGRSQKTISVNLNISLKTIETHRTRINKKLGMDNNRLIEEAKNWGLL"
BASE COUNT          693 a          396 c          422 g          569 t
ORIGIN      
        1 aaagctctgc acccggctga tgtctgcacc tttatcctta atcgtcttaa tgtcgaaaat
       61 cagatccgta agagatttca ttgctggatt tcgtagttcg taatttctgt cctgaatctg
      121 ttctacgctt cctttcattt ctttttcatc tgcatgataa cagtcaatgc agtttgccgc
      181 cgttctatga ccgtctttca tccagacgga aaactgcttc tccatcatat gacagtcggc
      241 acagttccct atctctgcat acaggaaggc ggaacatcct atactgaaca gaagcagaag
      301 catattccta aaagcattta ttttcatcgt aaccgaacaa atcataatca gcccaaagtg
      361 caaatcaaaa atgcatctaa agaataaaaa agtttaagat gtgcactctt ttcttgattc
      421 atttcaatta ttttcatttt catttcttac tctgcttttt tcagctgaaa acttaatcca
      481 gatgaatttc gaaatccacg aaaatcaagt cggcagaaat gaaatcatac cataattata
      541 aaaaaatacg cctaacgtgc acggttttca gttcttaaaa atagacagga gaatctgcat
      601 ctgctttgat aaccggagaa ccgatacagg ctgaaagaag tttctttatt cagatttcgg
      661 atgacaatat gactatgcag gaatgaacaa tgtacaacag atttgatgaa agactttcag
      721 aggaagcaga aaaattctac agatccggga caggaaataa gcttatgcag aagaccggaa
      781 aggtcggaaa aagaatgaaa tctgatcctg gagctgatga cgaccttcat ccccttctga
      841 atgcggcagg aatctggatg gaagaatatg cgagaagaaa aaaatggaac ggtgatgaac
      901 tttcggaaac atatattgcg atcactgaaa aaattcttca gattcttttc cgatttaaaa
      961 agaacggcta ttccaacttc ccctcctatc tgggaaaata cataaagaac tgcagaatga
     1021 acattatgcg gagagacttt gaaagagaca atcctgctct gtttgaactc tgggacgagg
     1081 aaggaaattt tcggagggca ttgacagccg aggagcctct gaagaaaaac cagaatctca
     1141 gaaatgctct tctggcactg aatccgctca acaggatggt tatttcaatg agatataatc
     1201 tctcttttat gaaatcggac gcggacacat tcttaaaact gatgaatgaa tccgacagga
     1261 cagagatgat gtgcagaaag gaaaagttat atcagtggga acagaaattt ctggaaagac
     1321 tgacaggtct tacaaaacga attcttgaaa gaaaggacaa tgagagaaag ctgctttccg
     1381 aaaggaaaaa aaggcttctg aaaaagctga tgagaccaag agaactgttt tcaattgctg
     1441 agctgggaaa aatatttgga atgccggaaa gcagaattgc aagagtctgc aaagtatcaa
     1501 tcagtcagat cagggaggaa tatgagaaaa gccttaagtc atctgactga agaatctcag
     1561 gggactcaaa agctgaaact gcttccttca agtcccctgt tctccccttt cctactcgca
     1621 cttatgcttt tgtctccgaa cataaacctt ggctgtgatt ttttttattc accgtgaacc
     1681 attcagattc tgttctattg tagttgacag aaaagtttct gcacttatgc ctgatccgat
     1741 taaaaaaagt gagaatattc ggggttttcc ctgaacgctt acagaagacc ccagttttta
     1801 gcctcttcaa tcagtctgtt attgtccatt ccgagcttct tgttgattct tgttctgtga
     1861 gtttctattg ttttgagaga tatgtttaaa ttgacagata tcgtcttctg cgatctgcct
     1921 gacagaaaga gtttcaatat ttcaatttcc ctgaagctca gtgccaaaaa aggatttgaa
     1981 ggatgattcc agatgaattc aagaccggga gaagaaaaag aagaatagag aaacggagag
     2041 agagaaaaca aaacgagaca ggagaaagaa caaaagagag
//
LOCUS       DAOQGJ010000639         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36389_length_2078_cov_4.045477, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000639 DAOQGJ010000000
VERSION     DAOQGJ010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36389_length_2078_cov_4.045477"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            711..>2078
                     /locus_tag="PK683_10470"
     CDS             711..>2078
                     /locus_tag="PK683_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3336 domain-containing protein"
                     /protein_id="HNH08917.1"
                     /translation="MEESDLEKAGSYREWQAIAYELDLQDDKIAWRKDDYSELFHSQL
                     LQEHIFLMKEYRRTHQGVKLIEAVQESLSRHSWELNSPKLYSEARSGTKFIIKEYFEE
                     IEKSVNFVCENSFNGISDHDKLRILSEGNRVHGNTALLLSGGASFGIYHLGVVKALLD
                     EDLLPNIITGSSMGAIVAAAACSKTDEELKEFFSDLTSIHRTAIRTNDFRTILSAGSV
                     MDPEQLIEHIESNIGNFTFKEAFEKTGRVLNISVSATRVRQRPRVLNHLTAPNLLIGQ
                     SALASCAIPGIFPAVTLMMKDRNGKTVPYMESEKWIDGSIHLDIPMQRIIRLHNVSRS
                     IVSQANPHVIPFVKEKENDNIITFIKDLITEAVQAQALKIMDLGVNLSEKMPWLSIVD
                     KIRSMMDQQYRGDINIYFPMTLESVTKIVSNPTQEEFEEYIRKGELAAWPKLEFIRDQ
                     MKLNRV"
BASE COUNT          653 a          444 c          439 g          542 t
ORIGIN      
        1 acgctccgcc cttcgggcta ttagaaatca aaaatggtag ggatgcctac cggcagcagc
       61 ttcccaactt gcgttggttt aacggggctt aagcagcccc ttcgctaaca ttgaagccgc
      121 taaaatcccc gcccttgcta gcctgcaagg cgacattcag tgtcgaccag aactgatttt
      181 aaaccaatat ttcattgatc ctatataatc taattagaaa tctgggaaat atcaggattt
      241 ccagcagcga agcagcgaag cggcattcgg cccgacacga ttctaaatcg gtgaaaccga
      301 aaatcaggac tcctgaatcc atcactgtcc attgcagacc ggaaaactct ttttccaaat
      361 tttactgtat atctgtccta ttagactcag tttccgtctc tctcaataaa aaacgggttt
      421 ttaatatcag tcaactctat tttccaaaac tgctcagact ttcagaagaa gctcaccgga
      481 ttaagagtct gtgtcacact tgcaaatata catttttcag aaatttgctt ttcaaaaaac
      541 agtaaaaagt cagaaacttc cagaaggttc agctgtttta tatagactct cttacaatat
      601 attcactgct gtgcaaaaaa aacagtgaga attcccaaag ttttccttat tggcacagaa
      661 atgcaccctt ccagaatgcc atatgtgaac acttaggcag gaaataccgt atggaagaat
      721 cagatctcga aaaagcaggc agttacagag aatggcaggc aattgcatat gaactggatc
      781 ttcaggacga caaaatagcc tggagaaagg atgattattc ggagctgttt cattctcagc
      841 ttcttcagga gcatattttc ctgatgaagg aatacagaag gactcatcag ggagtaaaac
      901 tgattgaagc ggttcaggaa agtctttcca gacattcctg ggaactgaat tcacccaaac
      961 tctattcaga agcgagatct ggaacaaaat tcatcattaa ggaatatttt gaagagattg
     1021 aaaaatcagt aaactttgtg tgtgagaatt cttttaatgg gatatcggat catgataaac
     1081 tgagaatact ttctgaagga aacagggtcc atggaaatac tgcacttctt ctcagcggcg
     1141 gtgcctcatt cggaatctat catctgggag tcgtcaaagc acttcttgat gaagatcttc
     1201 ttccaaacat catcacaggt tcaagcatgg gagcaattgt tgctgcagct gcctgcagca
     1261 agactgatga ggaactgaaa gagttttttt cggatctgac ttcgattcac aggacagcaa
     1321 tccgcacgaa tgatttcagg acgattctga gtgcaggttc tgtcatggat ccggagcagc
     1381 tgattgaaca cattgaaagc aatatcggaa attttacatt caaggaagca tttgaaaaaa
     1441 caggacgtgt tctgaacatc tctgtttccg caaccagagt gcgccagaga cccagggtac
     1501 tgaatcatct gactgctcca aatctgctga taggacagag tgctctcgct tcctgcgcaa
     1561 ttccgggaat tttccccgct gtcactctga tgatgaagga caggaacggg aaaacagttc
     1621 cgtacatgga atcggagaaa tggatagacg gatcgattca cctcgatatc cctatgcaga
     1681 gaatcataag acttcataat gtaagccgtt caatagtctc gcaggcaaat cctcatgtga
     1741 ttccgttcgt aaaggaaaaa gaaaatgata atatcattac attcataaag gatttaatta
     1801 ctgaggccgt tcaggctcag gcactgaaaa tcatggatct gggagtgaat ctttcagaaa
     1861 aaatgccctg gctttccatt gtagacaaga tcagatccat gatggatcag cagtacagag
     1921 gagacatcaa tatctatttc ccgatgacac ttgaatcagt gaccaaaatt gtttccaatc
     1981 cgactcagga ggaatttgag gaatacatcc gaaaaggaga acttgctgcc tggccgaaac
     2041 tcgaattcat ccgcgatcag atgaaactca acagggtt
//
LOCUS       DAOQGJ010000640         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36395_length_2078_cov_3.347504, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000640 DAOQGJ010000000
VERSION     DAOQGJ010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36395_length_2078_cov_3.347504"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..338
                     /locus_tag="PK683_10475"
     CDS             132..338
                     /locus_tag="PK683_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08918.1"
                     /translation="MKYRPGKLLFERYSGGASNFVLIVKTLLSFPGKYTSGVRGIFSA
                     SFQPLSSFSRWVKLSENLSITYQG"
     gene            830..1588
                     /locus_tag="PK683_10480"
     CDS             830..1588
                     /locus_tag="PK683_10480"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH08919.1"
                     /translation="MTVLFSDIRSFASLIEGMTALEISEFTDSYNAAMKPVIQRYKGF
                     IEKNIGNAIMAFFPEKPSNAIDAAIAMLNELHQINLKRKEKELQLISIGIGIHTDIQP
                     KEVLNGRSKALIYGAASMAARLEELTKAYSSSLLVSMDVMDRVENKDIYYFRHVDTVK
                     AKKEDKSVHILEILNGNSQRIIDIKLKTREAFERGIYLYHSKEFIYALKCFKYVLKDD
                     SRDTAAEIYVNRCNEYLEKGVGSDWDGVEQLENK"
     gene            complement(1670..>2078)
                     /locus_tag="PK683_10485"
     CDS             complement(1670..>2078)
                     /locus_tag="PK683_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08920.1"
                     /translation="RVAMGLVLFTLIGHTVGTFMKVPAEQTEVAKTVEIMKTTLVPMP
                     VGSPKSYSEIFLGTNLTVSLFLFVSGICFWISSSEGGLDGKGKVFLAVNSFGAAMLSL
                     ISFLYFFPLPAICTGIAAVLGFLVYSKKQETAR"
BASE COUNT          685 a          391 c          413 g          589 t
ORIGIN      
        1 aaagtctgct gctcagcggg attaaaacat ttcttccata tctgctgttg atgattttcc
       61 cgttttcaga tttcccgtca ctgtacacga atgttccgtt caaatagact gaataaactt
      121 ctcctaatga aatgaaatac agacccggga agcttttatt tgaaaggtat tctggaggtg
      181 catcaaattt tgtcctcata gtaaaaacac tgctctcttt tccaggaaaa tatacttccg
      241 gggtcagggg aattttttcg gcaagtttcc agcctttatc ctcattctct agatgggtaa
      301 aactatcaga aaatttatca attacatacc aaggatgaaa actgcattca atgtgcttat
      361 ttctatccct gcagtcctca gaaaggttta ttttttcaat tgagaaaatt ggaaaggaga
      421 aaagaacggt cagctgcagt aatctggaaa gaagtttatg catattccga aaaaatacgg
      481 ttcatccaga tgctgtcaag taagttattt acagacgaat cagctgattt atgatggatg
      541 cgatattata ggggaatgtc agcattcagg tgaaattttt accaaattcc gtctaaagtt
      601 atctcttgac gaatattccg caaaatctta tcttataaat cagtcagcag cttttaaaaa
      661 aacaggtttc gatgcagtaa aaactgatga aacggaacag tctctctgaa ttgttttgca
      721 gaaaattttc gtctgtaaac ttgatgcact gtttcatcct gccctcagag ttttttcagc
      781 agaggaatca ttacacaaat aaaactttgg ggttcattgc aaaaatagta tgacagtctt
      841 attttcagat attaggtctt ttgcatctct cattgaagga atgactgctc tggaaatttc
      901 agaatttact gattcctata acgcagcgat gaaaccggta atacaaagat acaaagggtt
      961 catcgaaaag aatatcggaa atgcaattat ggcatttttt ccggaaaaac cctccaatgc
     1021 aatagatgca gcaattgcaa tgctcaatga actgcatcag ataaatctga aaagaaagga
     1081 aaaggaactg cagctgattt ctatcggaat cgggatacat acagatattc agccgaagga
     1141 agttctcaat ggaagaagta aagcattgat ctacggtgcg gccagtatgg cagcaagact
     1201 ggaagagctg acaaaagcct attcatcttc tcttttggta agtatggacg taatggacag
     1261 agtagaaaat aaagatatat actatttcag acatgtagat acagttaaag ctaaaaagga
     1321 agacaaatca gttcatatcc ttgaaatcct gaatgggaac tcccaaagaa ttattgatat
     1381 caaattgaag actagagagg catttgagcg ggggatatat ctttatcatt cgaaagaatt
     1441 tatctatgca ctgaaatgct tcaaatatgt tttgaaggat gactctcggg acactgccgc
     1501 tgaaatttat gtgaacagat gcaatgagta tctggaaaaa ggagtcggct cagactggga
     1561 cggagtggag cagctggaaa ataaataatt cacctggatg cggctccgct gactgtgaaa
     1621 ctcacccagt tttcagccct gctttataaa ctcacttata atatgttttc tatcgggcag
     1681 tttcctgctt ttttgaataa actagaaaac ctaagactgc ggcaatacct gtgcagattg
     1741 caggcagagg aaaaaaatac agaaagctga ttagagaaag cattgctgca ccgaaactgt
     1801 tgactgccag aaaaactttt cccttgccgt ccaatcctcc ctccgaagag gaaatccaaa
     1861 aacagattcc ggaaacgaaa aggaaaagac tcactgtcag atttgttccc agaaaaatct
     1921 ccgaataact ttttggagat ccgacaggca ttggaaccag tgttgtcttc ataatctcga
     1981 cagtttttgc aacctctgtc tgttccgcag gaactttcat aaaagtcccg actgtatgcc
     2041 cgattaaagt aaataaaacg agccccattg cgactcta
//
LOCUS       DAOQGJ010000641         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36485_length_2075_cov_3.665347, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000641 DAOQGJ010000000
VERSION     DAOQGJ010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36485_length_2075_cov_3.665347"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..620
                     /locus_tag="PK683_10490"
     CDS             246..620
                     /locus_tag="PK683_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HNH08921.1"
                     /translation="MNLSTRIVQVSLFLVGAIALFGGTLQMILGQPNTTPRLDNIHRF
                     MAGVYLSMGMISIWTAWTVRTQSTLIYLIALGVFLGAVGRLVSIAAVGLPEPASVWIG
                     YLVPELVFPFIILAAQTVRNRK"
     gene            complement(651..1817)
                     /locus_tag="PK683_10495"
     CDS             complement(651..1817)
                     /locus_tag="PK683_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HNH08922.1"
                     /translation="MKHSKPFFQDVTLRDGNQALPKPWNQQEKEKVFRRIIELGIQGA
                     EVGFASASDTDFASCRRLAEIAPESITIASLSRANEKEITLSWDAIKYSARPRIHIVF
                     PVSRNIIENVIRTSKEDAIQKVSNAIRFARNLAGSRGTVQFSGEHFGECRNNLEYALD
                     FFEEAVQQGADVINFPNTVERFRPFEFIRMLSAGVERFRDRAVLSVHTHNDLGMATAT
                     TVESFFAGVVQMETTLGGLGERAGNTNFFEVACALHNCGIETGINYHYISDTAEMISE
                     MSGIPVHPKAPLVGSEVFSHRSGIHQDGVAKTIKSQQNAYGTVAPEFIGRKDGHKIRF
                     TSQSGKSAIQYMLESRGIEITEEQARKFQPILKKISEENGELDEEKIAMVLAEG"
BASE COUNT          498 a          498 c          430 g          649 t
ORIGIN      
        1 actgtttttt cgattgaatc cattgactgt attctagaac cgagtgaagt gtactccgga
       61 gttgattggg aaacggatcc ttcctgacgc atctaggcaa gactctgttc tgccgggact
      121 gaccaccttg ctcactttgc ttctgtttcc gatatttcca gcggtgacag aaactggtgt
      181 aactgagaaa ctcttgacta aaactgttcg gtcaatattt tgctttaatg aatgaaaatg
      241 caaatatgaa tctcagtaca agaattgtac aggtttccct ctttttagtc ggtgccattg
      301 cacttttcgg gggaactctg cagatgattc tcggtcagcc gaacacaaca cccagattag
      361 acaacataca ccgatttatg gccggtgtct atctgtcaat gggaatgatc agcatttgga
      421 cggcctggac ggtacggact cagagtactt tgatttacct gatcgcactc ggtgtttttt
      481 tgggagctgt cggaagactt gtttccattg cggctgtagg tcttcctgag cctgcgtctg
      541 tctggatagg atatcttgtt cctgaactgg tatttccttt cataatattg gctgcacaga
      601 cagtcagaaa ccgaaaatga aagcaggaat ttgaattcct gcttctgctt tcatccttct
      661 gccagaacca ttgcaatctt ttcctcatcg agttcaccgt tctcctctga aatcttcttc
      721 agaataggct ggaactttct ggcctgttct tcagtgattt ctattcctct gctttcgagc
      781 atatactgta ttgcggattt ccctgactga ctggtgaaac ggatcttatg accgtccttt
      841 cgtccaatga attccggtgc aacagtacca tatgcattct gctgactttt gattgtcttt
      901 gcaacgccat cctgatgaat cccgcttctg tgtgagaaaa cctcgcttcc taccagaggt
      961 gctttcggat gcacagggat gccgctcatt tcggaaatca tctctgctgt gtctgaaatg
     1021 taatgataat ttataccagt ttcaattccg cagttgtgca gtgcacaggc cacttcaaag
     1081 aaatttgtat ttcctgctct ttcccccagg cctcccaggg tggtttccat ctgcaccacg
     1141 cctgcaaaaa aactttccac agttgtggca gttgccattc ccagatcgtt gtgagtatgt
     1201 accgacagaa ctgccctgtc tctgaatctc tcgactcctg cggacagcat tcgtatgaat
     1261 tcaaagggtc tgaatctttc cactgtgttc ggaaaattga tgacatctgc tccctgctgt
     1321 actgcttctt caaaaaagtc caatgcatat tccagattgt ttctgcattc tccgaaatgt
     1381 tctcctgaga actgaactgt tcctctgctc cctgccaggt ttcttgcaaa acgtattgca
     1441 tttgatacct tctgtattgc atcttctttg gaagtccgta tcacattttc tatgatgttt
     1501 ctgctgacag gaaatacgat gtgaattctc ggtcgggctg agtattttat cgcgtcccag
     1561 gaaagagtaa tttccttttc attggcacgg gacagacttg ctattgtgat actctccgga
     1621 gcaatttcag ccagtctcct gcaggatgca aaatcagtat cgctggcact tgcaaaaccg
     1681 acttcagctc cctgaatccc caactcaata attcttctga atactttttc tttttcctgc
     1741 tgattccaag gcttcggcag tgcctgattc ccgtcacgca atgtgacatc ctgaaaaaaa
     1801 ggttttgaat gtttcataaa ttcctccaaa tttctgctct gcagaagggg agggcacaaa
     1861 aagaaaaagc ccgcccccga tcagggagca ggctttttct tatgatgaaa tcacacttct
     1921 ccctgtctat ccaagaagga gaagcagaag tgaagagtgt gacttgatca taaattcacg
     1981 ctgaaataac ggatgaattc tgtcaaggaa ttttttcaat gaattcactc cccaagcacg
     2041 gcaagtgact gttctgcaat ttccagttct tcgtt
//
LOCUS       DAOQGJ010000642         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36490_length_2075_cov_2.970792, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000642 DAOQGJ010000000
VERSION     DAOQGJ010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36490_length_2075_cov_2.970792"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK683_10500"
     CDS             complement(<1..566)
                     /locus_tag="PK683_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH08923.1"
                     /translation="MKRYMIALFILLSYCRQHSHEKKMDLKLYAFSYGTSLYSGKLMN
                     TENRDKNFKIQWLFYLLVDDDRRILVDTGFSDADYVTAFGLRDFRIPKDVIEAGNFRS
                     DDITDIVITHSHFDHIGGADSFKNAKIHITEREFQEFKRSSLYGRRRAFYDSKEKNNE
                     ILFTDNGQRLGSSLELVHSNGHTTGSQA"
     gene            753..>2075
                     /locus_tag="PK683_10505"
     CDS             753..>2075
                     /locus_tag="PK683_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH08924.1"
                     /translation="MNSNTELELAYSYVQYTDKNIFLTGKAGTGKTTFLQNIKKNPLK
                     RMITVAPTGVAAVNAGGVTIHSFFQISFGPQITGLNSVNNSENQFRKFSREKINIIRS
                     LDLLVIDEISMVRADLLDAVDGVLRRYKDPYEPFGGVQLLMIGDLQQLSPVIKDEEWR
                     ILQEHYESMYFFGSHALRQTDYVCIELRTVYRQTDNKFIDILNRVRENSADSEVLKEL
                     NRRYIPDFERQSIPGYITLTTHNSKANEINSFRLENLPEKEFSFEASVEGEFPEYSYP
                     ADFRLVLKEGSQVMFVKNDSSREKLFYNGKIGIIQKINPGSIEVLSPGEEQTVTVEKA
                     VWENIKYRINEETKEIEETKVGTFSQYPLKLAWAITIHKSQGLTFEKAVIEADRAFTH
                     GQIYVALSRCKSLEGLVLSSPIAARSIMCDPGILEYSREKLRKTPDERD"
BASE COUNT          655 a          420 c          457 g          543 t
ORIGIN      
        1 aatgcctgag accctgttgt atgaccgttg gaatgaacca gttccagaga ggagccaagt
       61 ctctgtccgt tgtcagtaaa caaaatttca ttattttttt ccttggagtc atagaaggcc
      121 ctgcgcctcc cgtaaagact gcttctttta aattcctgaa actctctttc tgtaatgtga
      181 atctttgcgt ttttaaagga gtcagctccg ccgatatggt caaaatgact gtgcgtaatg
      241 actatatctg taatatcatc tgatcggaaa tttcctgctt caataacatc cttgggaata
      301 cggaaatccc tgagtccgaa tgccgtgaca tagtctgcat cactgaatcc tgtatcgacg
      361 aggatccttc tgtcatcatc tacaagaagg taaaaaagcc actggatctt gaaatttttg
      421 tctctgtttt ccgtattcat cagtttgcct gaatacagag atgttccgta agaaaatgca
      481 tacagtttca gatccatttt cttttcatgg ctgtgctgtc tgcagtaaga gagcagaata
      541 aacagtgcaa tcatatatct tttcataccc tggcttttcc cccgaaagag tcgtgaatcc
      601 tttattatat gacggaaaaa aatctcaaaa aaagacaaaa atttcgttct tatagaattc
      661 tgtcaatgcc tatgaatgcg gaatgtgaat tgtcagtttt tttcagacaa atccgattga
      721 cctctatttc cgcacactgc agatttgacc aggtgaacag caatacagaa cttgaactgg
      781 cttacagcta tgtccagtat acagataaga acatatttct gacaggaaaa gcagggacag
      841 ggaagactac attccttcag aacatcaaaa agaaccctct gaaaaggatg attactgtcg
      901 ctccgacagg cgttgccgcc gtcaatgcag gcggggtcac aattcattcc ttttttcaga
      961 tatccttcgg ccctcagatc acggggctga actcagtaaa caattcagaa aatcagttca
     1021 gaaaattcag tcgggaaaaa ataaacatta tcagaagtct cgatcttctg gtcatagacg
     1081 aaatcagtat ggtaagagca gatcttctgg atgcagtgga cggagttctc aggaggtata
     1141 aagatccgta tgaacccttc gggggagttc agcttctgat gatcggcgac ctgcagcagc
     1201 tctcgccggt catcaaggat gaggaatgga gaatccttca ggaacactat gaatcaatgt
     1261 actttttcgg aagtcatgca ctcaggcaga cagattatgt atgcattgaa ctgaggacag
     1321 tttatagaca gactgataat aagtttattg atattttaaa cagggtcaga gaaaacagcg
     1381 cagattcaga agtactgaag gaactgaaca gaagatatat tcctgatttc gaaagacaga
     1441 gcattccggg atatattacc cttacaactc acaattccaa ggcaaatgaa atcaacagtt
     1501 tcagactgga aaacctgccg gaaaaagaat tcagctttga agcttctgta gaaggggaat
     1561 ttccggaata ctcctaccct gccgatttca ggcttgtact gaaggaaggc tctcaggtaa
     1621 tgtttgtgaa aaatgacagt tcccgggaaa agctttttta taacggcaag ataggaatca
     1681 tacagaaaat aaacccaggc agcatcgagg tattaagtcc gggagaagaa caaactgtga
     1741 cagtggagaa agctgtctgg gaaaatataa agtaccggat aaatgaagaa acaaaggaaa
     1801 ttgaagaaac aaaggtcgga actttctctc agtatcctct gaagcttgca tgggcaatca
     1861 cgattcataa aagtcagggg cttacattcg aaaaggcagt cattgaagca gaccgggcat
     1921 tcactcacgg acagatatat gtggctctga gcagatgcaa gtcactggaa ggtctcgtac
     1981 tgagttcacc catcgctgcc agaagcatta tgtgcgatcc gggaatactg gaatacagca
     2041 gggaaaagct ccggaagact cctgatgaaa gggat
//
LOCUS       DAOQGJ010000643         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36518_length_2074_cov_2.892026, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000643 DAOQGJ010000000
VERSION     DAOQGJ010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36518_length_2074_cov_2.892026"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK683_10510"
     CDS             <1..547
                     /locus_tag="PK683_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08925.1"
                     /translation="GREICSLRGENVSSIAFSPEDRHILSGHSDGSFSVWDMESCRKN
                     INYYPAKVWKLHEAGVSSFQFLSSGEVLLVSYLNPESPEKNILKYFKWSLIESFRKGD
                     ISNEKFLLAEQRFFSDGSILVTSPSGKFDRFPYSAEELYETAADPESGKKFNPDRITK
                     NREENMLEKLLLQGFELEDFE"
     gene            complement(836..1090)
                     /gene="rpmA"
                     /locus_tag="PK683_10515"
     CDS             complement(836..1090)
                     /gene="rpmA"
                     /locus_tag="PK683_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNH08926.1"
                     /translation="MAHKKGGGSSKNGRDSASQRLGLKRSGGQLVKAGNILVRQRGTE
                     FRPGKNVGIGKDHTLFALIQGIVKFEYVTKKRQQISVYPA"
     gene            complement(1408..1716)
                     /gene="rplU"
                     /locus_tag="PK683_10520"
     CDS             complement(1408..1716)
                     /gene="rplU"
                     /locus_tag="PK683_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004508248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNH08927.1"
                     /translation="MYAVITIGNQQFKVKQGDVFLSQKTGKEQGAEFESPVHLLAQDN
                     KVHIGSPVVNGAKVVLKVLEDLRGQKIKGFKYKRRKNYRRRWGHRQSLQKLQVVSISA
                     "
     gene            1809..>2074
                     /locus_tag="PK683_10525"
     CDS             1809..>2074
                     /locus_tag="PK683_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH08928.1"
                     /translation="MEIRNICIIAHVDHGKTTLLDGILRETGAVTSKELRERIMDSND
                     LEQERGITIVAKNTAVLYKNTRINIVDTPGHADFAGEVERVLNMA"
BASE COUNT          587 a          416 c          440 g          631 t
ORIGIN      
        1 aggcagggag atctgttctc ttcggggaga aaatgtatcc tctattgcat tcagtcctga
       61 agacaggcat attctttcag gacattctga cggaagtttt tctgtatggg atatggaatc
      121 atgcagaaaa aatataaact attatcctgc aaaagtctgg aaactccatg aagcgggagt
      181 cagttccttt cagtttctgt cttcaggaga agtgcttctc gtgtcttatc ttaatcccga
      241 aagtcctgaa aagaacatac tgaagtactt caagtggagt ctcattgaat cattcagaaa
      301 gggagatatt tccaatgaaa agtttctgct tgcggagcag agattttttt cagacggatc
      361 gattttagtc acttcaccgt ccggaaaatt tgacagattt ccttattctg cagaggaact
      421 gtatgagact gcagcagatc cagagagcgg caagaaattc aacccggaca gaatcacaaa
      481 aaacagggag gaaaatatgc ttgaaaagct gcttcttcag gggtttgagc ttgaggattt
      541 tgagtgagct ttgatacttt cttgcggaaa agaatataat tttcagagtc tgcaattttg
      601 tctgaactgt cagagaagtc atgcagagga gtttgggaca gatcagtaaa aacaggaact
      661 ttcaggctgt agctttgtat ggcggcagat ctcaatgctt ccagaactat tcactaaata
      721 aattgataat atagatatat gcagaatgaa attcgaatga ctgagttttt taatattgaa
      781 agctggagga agggaaaaaa ataaatgccc atcttatgaa tgggcagatc gaatattaag
      841 caggataaac tgagatctgc tgtctttttt ttgttacgta ttcaaacttg acgattccct
      901 gaatcagtgc gaacagggta tggtcttttc cgattcctac attctttcca ggacggaatt
      961 cagtccctct ctgtcttacg aggatattac cagcttttac aagctgaccg cctgaacgtt
     1021 tgagtccaag tctctgcgac gctgagtcac gaccgttttt agatgaaccg ccacctttct
     1081 tgtgtgccat agttttctcc tatatcattt gtattacaat caatcccggt tcctgagctt
     1141 ttaacttttc aagaccggtt aaaaccattt ccatacagct ctctgtctga atatcaggtt
     1201 ctttcagtag gattttcaga aacccatttt ggacgtctga aaactccagt ttatttttct
     1261 tcagcaggta tagaactaaa gtctgtccaa gcatagaaac tgcggcacag actaagtttt
     1321 ctcctttttt tccgtaggaa tcaggggagt gtccttgtac tgtcaattgg acaaaaaaat
     1381 cgtgttctct cctgaattcg gcagaaatca agcgctgata gaaaccacct gcagtttctg
     1441 aagagactgt ctgtgtcccc agcgtcttct gtagtttttt cttcttttgt atttgaagcc
     1501 tttgattttc tgacctctca ggtcttccaa cactttcaaa actactttgg caccgtttac
     1561 aacgggtgat ccgatatgaa ctttattgtc ttgagccaat aaatgtacag ggctttcaaa
     1621 ttcggctccc tgttcttttc cagtcttctg ggaaaggaat acatccccct gcttgacctt
     1681 aaactgctga ttgccgatag taattactgc gtacatagtt ttctctcttt aggccatgat
     1741 ttttttgagt caggccgtgt caacagcaaa aggctggact tctcatccct gtctaaattc
     1801 ttggcagtat ggaaattcga aatatctgta tcatagctca cgtagaccac ggcaagacca
     1861 ctcttctgga tggaattctc agggaaacag gagctgtcac ctctaaggaa ctgcgcgaaa
     1921 gaatcatgga ttcaaatgat ctcgaacagg aaagagggat tactattgta gcaaagaaca
     1981 cagctgtatt gtataaaaat accagaatca atatagtgga cactcccggt cacgcggatt
     2041 ttgccggaga ggtagaaagg gttctgaata tggc
//
LOCUS       DAOQGJ010000644         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36580_length_2072_cov_2.572137, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000644 DAOQGJ010000000
VERSION     DAOQGJ010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36580_length_2072_cov_2.572137"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1368
                     /locus_tag="PK683_10530"
     CDS             298..1368
                     /locus_tag="PK683_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNH08929.1"
                     /translation="MKTKRFRLGELFSGPGGLGYAAVNSLSDDGKYSVSHEWASDFDR
                     DSCDTYARNICPENPESVICKDVRKLDINSLNPIDAFAFGFPCNDFSMVGEQKGLDGS
                     YGPLYTYGVKVLKKFQPQWFLAENVGGLRNSNEGKAFQMILTALFDSGYDVFPHYYKF
                     ERYGVPQNRHRVIIIGIRKDLHLRFGIPSSKKYSDWDISAKKALTVPTIPSDAPNNEL
                     TKQSQAVIERLKYIRPGENAFNADLPEHLRLNVKGAKISQIYRRLDPGKPSYTVTGSG
                     GGGTHVYHWSENRALTNRERARLQTFPDHFVFCGSKEKVRKQIGMAVPVQGAKVIFKA
                     VLNTFAGISYETEEPSIEPLVH"
     gene            1488..>2072
                     /locus_tag="PK683_10535"
     CDS             1488..>2072
                     /locus_tag="PK683_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08930.1"
                     /translation="MKSEKELEREEREKFRYADFARIVFSILVLYISRNVSAVNILMS
                     LHLLISVLWLVLSEMHPLFQRKYSAIWLVPASLDVLNCLMIIYVTGSSYSSWILSLTF
                     ITAISASDPLWWRGAFTGIAGAAGLALMLVLVQTGVLPFTDIWKINYRPNSWSLVAQT
                     VALNAGVSFFVWAAVHKSSVIADTGRTAAENESSW"
BASE COUNT          590 a          400 c          482 g          600 t
ORIGIN      
        1 tcataaaaga aaaatctgtc ctgggtaaag gagacaacgg tcatacagtc ggaaaggaaa
       61 aacagaacaa gtattccaat tctgtttaca gtctgtctga tatatttcat aaagaactta
      121 ttattcagta aaaagaaaat gtcaaggatt aatgggtgtg catccctggc gttaattttt
      181 aaaaaccact tgtatttctg gctcagtgat tttcattgca actatgtctt ggaaagaatt
      241 ctttaattca gctgatctgc ctgcaggaag ttttcggagt attgccggtt cttgctcgtg
      301 aagacaaaaa gattcagact gggtgagctt ttttccgggc cgggggggct gggttatgct
      361 gccgtaaatt cgctttcaga tgacggtaaa tattcagttt cccatgaatg ggcaagcgat
      421 tttgaccggg attcctgtga tacctatgca aggaatatct gtccggaaaa tccggaatct
      481 gtcatctgca aggatgtcag aaaactggat ataaattcat taaatcctat tgatgcattt
      541 gctttcggct tcccatgcaa tgatttcagt atggtggggg agcagaaggg actcgacgga
      601 tcgtatggtc ccttatacac ttatggagtc aaggttctga aaaagtttca gcctcagtgg
      661 ttccttgcag aaaatgtagg cggactcaga aattcaaatg aaggcaaggc ctttcagatg
      721 atcctgactg ctctttttga ttcagggtat gatgtgtttc ctcattacta taagtttgag
      781 agatacggtg taccccaaaa tagacacaga gtaattatca ttggcatacg aaaggatctg
      841 catctccggt tcggaatccc ttcttctaaa aaatattctg attgggatat ctctgcaaaa
      901 aaggcactga ctgttcccac gattccttcc gatgcaccga acaatgaact gacaaagcag
      961 tctcaggcag tcattgaaag actcaaatat atcagacctg gagagaatgc attcaatgcg
     1021 gatcttcctg agcatctgcg cttaaatgta aaaggggcaa agatcagtca gatttacaga
     1081 aggcttgatc cgggcaaacc ttcctatacg gtgacaggca gcgggggagg cgggactcat
     1141 gtctatcact ggtcggaaaa cagagcgctt accaacagag aaagagcaag acttcagaca
     1201 ttccctgatc attttgtatt ctgcggctcc aaggaaaaag tgcgcaagca gatcggaatg
     1261 gcagttcccg ttcagggagc aaaagtgatt tttaaggcag ttttaaatac attcgcagga
     1321 atttcctatg aaacggaaga accgagcatt gagcccctgg tgcattgact ctgatctgaa
     1381 gatgatattc atacggataa tgtctgaaaa attattatct ttcccggatt ttatttgaaa
     1441 atcagaatat cggttcattc tttggttttc aaggatgatc ttctgttatg aaaagtgaaa
     1501 aagagttgga acgtgaagaa agggaaaaat tcagatatgc tgattttgcc cgaatagttt
     1561 tcagcatttt agtactgtac ataagcagaa atgtttcagc agtaaatatt cttatgagcc
     1621 tgcatctttt gatttcagtc ttatggctgg tgctgagcga aatgcacccg ctctttcaga
     1681 ggaaatattc cgcaatctgg cttgttccgg ccagtctgga tgtgttgaac tgcttaatga
     1741 tcatctacgt tacaggctct tcttattcaa gctggattct gtcattgaca ttcataactg
     1801 ccatttccgc gtccgatccg ctctggtgga gaggtgcatt cactgggatt gccggggctg
     1861 cagggcttgc attgatgctg gtattagttc aaacaggagt cctgcctttt acagatatat
     1921 ggaaaataaa ttatagaccg aattcatgga gccttgtcgc tcagactgtc gcattgaatg
     1981 caggtgtttc tttttttgtc tgggccgcag tccataaaag ctcagtcatt gccgatacag
     2041 gcagaactgc cgcagaaaat gaaagttcct gg
//
LOCUS       DAOQGJ010000645         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36626_length_2070_cov_3.600000, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000645 DAOQGJ010000000
VERSION     DAOQGJ010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36626_length_2070_cov_3.600000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PK683_10540"
     CDS             <1..225
                     /locus_tag="PK683_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08931.1"
                     /translation="EGTSCKSQDFRRAETNAPFFDFVSTDITCGIHFKNSSHTAGYFL
                     SISGADEVNTPLLPDGKLHIGNKTGLTVKD"
     gene            406..>2070
                     /locus_tag="PK683_10545"
     CDS             406..>2070
                     /locus_tag="PK683_10545"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH08932.1"
                     /translation="MEMAEYREVLNKSVPVRIFNWICAKGGYAFACLVFASLCTWIGI
                     CYVYFSYMGAVPDWNFEEYKKEFYFIYTVVVCSNIFLIYILGGFYKLGINTYIGPINL
                     ANRKLGGINFFKEPVSNAEVAELLQNFYSLQSYIMKICTILYAVNIVLYGAYTFYLKR
                     DIMEIVRAAGIVIPCVLIFSGFIYLTMDTLFALTRVFLKKEILSRKISMPPDMPGMSM
                     KFKFMFLLATIFVSIFNMAAFVSSAKRPLFMIALYILLSIVSCGLLLFFFFLQIDTTL
                     KQINESASDLAKGGDGHLPMISRDEEFVIFALNYEIAVAEVKDIRTNLEKKVKEKTSE
                     LSKALDLTQRLKEQQDGDYFLTTLLLEPLNVFESDSRYVSIESFIHQKKVFDFKGRQK
                     EIGGDLNFSCQIELQGEKYTFAVNADAMGKSMQGAGGVLVFGASLRSIIERTRVSPEM
                     QHYSPEFWLKNMIEEVHRLFLSFDGSMLISLFTALIHESSGFTLYTCAEHPEGVLYRD
                     GKASFLKVNSNFMKIGVTMDQEDLKLDHILLRNGDILVMGSDGRDDI"
BASE COUNT          573 a          414 c          466 g          617 t
ORIGIN      
        1 gaaggaactt cctgcaagtc tcaggatttc agacgggcag aaacaaatgc tccctttttt
       61 gatttcgtca gtaccgatat tacctgcggg attcatttca aaaactcctc tcacactgcg
      121 ggatattttc tctcgatcag cggagcggat gaagtaaata cgcctcttct tcctgacgga
      181 aaacttcata ttggaaacaa aacaggcctg accgtaaaag actgattctg catttgcagg
      241 aactgccgac cgatgcaatt ttccttttcc gaaatacttc tttagtttac agataaacag
      301 catctgtcag aatttacaat tggatttaac ggataacggg aaggcggtac tgagaccgag
      361 agcggatatt ctttgcacag tctcgtctcc ccggcgggaa cctttatgga aatggcagaa
      421 tacagagaag tcttaaacaa atcagttcca gtcagaatat tcaactggat ctgtgcaaag
      481 ggaggatatg cctttgcatg tctggttttt gcgagcctgt gtacctggat cgggatatgt
      541 tatgtatact tttcctatat gggtgcagtt cctgactgga attttgaaga gtataaaaaa
      601 gagttttatt tcatttatac agttgtagtc tgcagcaata ttttcctgat ctatatcctc
      661 ggcggatttt acaagcttgg gatcaataca tatatagggc cgatcaatct ggcaaaccgg
      721 aagctgggcg gaatcaattt cttcaaagaa ccggtgtcca atgcagaggt ggcagagctt
      781 ctgcagaact tctacagtct tcagtcctac ataatgaaaa tctgcaccat actttatgca
      841 gtgaatatcg ttctttacgg tgcatataca ttttatctca aaagggatat tatggagatt
      901 gtgcgtgctg cggggattgt aatcccatgt gtgctgattt tttcaggatt catctatctg
      961 accatggaca ctctctttgc tctgactagg gtcttcctga agaaggaaat actgagcaga
     1021 aaaatcagta tgcctcctga tatgcctgga atgagtatga agttcaagtt tatgtttctt
     1081 cttgccacca tttttgtatc catattcaat atggctgctt ttgtttcgag tgcaaaaaga
     1141 cctttgttca tgattgcact ctatattctt ctttcaatcg tctcctgcgg ccttctgctt
     1201 tttttcttct ttcttcagat agacactaca ctcaaacaga tcaatgagtc tgcaagcgat
     1261 ctggcaaagg ggggagacgg gcatctcccg atgatttcga gagatgagga gtttgtgatt
     1321 tttgcactga attatgaaat tgcagttgct gaggtaaaag atatccgcac aaatctggaa
     1381 aagaaagtga aagaaaaaac gtcagaactt tccaaggcac tcgatctcac tcagcgtctg
     1441 aaagagcagc aggacggtga ttatttcctg acgactctgc ttttggaacc tctgaatgta
     1501 tttgaatcgg attccagata tgtatccata gaatctttca tacatcagaa aaaagttttt
     1561 gacttcaagg gcagacagaa ggaaatcggg ggtgatctga atttctcgtg tcagattgaa
     1621 cttcagggcg agaaatatac atttgcagtg aatgcagatg caatggggaa atctatgcag
     1681 ggagcagggg gcgtgcttgt attcggcgca tctctgcgct ccatcattga aagaacaagg
     1741 gtctctcctg aaatgcagca ttattctcct gaattctggc tgaagaatat gattgaagag
     1801 gtccaccgtc tgtttttaag ttttgacgga tccatgctga tctctctttt cactgctctc
     1861 atccatgaaa gctccggatt cactctgtac acctgtgcag aacatccaga aggagttctt
     1921 tacagagacg gcaaggcctc attcctgaaa gtaaattcga attttatgaa gatcggtgtg
     1981 acaatggatc aggaggatct gaagctcgat catatcctgc tcagaaacgg agatatcctt
     2041 gtaatgggtt ctgacggaag ggatgatatt
//
LOCUS       DAOQGJ010000646         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36627_length_2070_cov_3.485360, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000646 DAOQGJ010000000
VERSION     DAOQGJ010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36627_length_2070_cov_3.485360"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PK683_10550"
     CDS             <1..409
                     /locus_tag="PK683_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNH08933.1"
                     /translation="VDLIVTSPPFLDKVNYAADNWMKLWFLGIPDEEAASLSILPTID
                     AWSKFIHGTLREASRVLKKKANIVMEVGDVDYHGKLVNLDEIVINMSRDTGLHWTRTY
                     INSQKFTKLSNCWNISNNEKGTNSNRCVVLQKS"
     gene            484..2031
                     /gene="thiC"
                     /locus_tag="PK683_10555"
     CDS             484..2031
                     /gene="thiC"
                     /locus_tag="PK683_10555"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNH08934.1"
                     /translation="MNIQEPAELSVPSKKITLTNGETVSLYCTEGPENTDLKKGLPKR
                     RAEWIQKRKDRGDRNFSQMHYARKGEITEEMRFIGIRENVSPDFVRDEVARGRAIIPA
                     NINHTELEPMIIGRNFLVKINANIGNSALASSIEEEVEKLKWATKWGADTVMDLSTGK
                     NIHETREWILRNSPVPIGTVPIYQALEKVKGKAENLNIDIFLETLIEQAEQGVDYFTI
                     HAGVLLRYVPMTAKRTTGIVSRGGSILAKWCLAHHKENFLYTHFDDICEIMKKYDISF
                     SLGDGLRPGCIADANDEAQFSELKTLGELTQRAWKHDIQVMIEGPGHVPMHMIKENME
                     KQLEICHEAPFYTLGPLVTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPN
                     RDDVKTGVITYKIAAHAADLAKGHPGAQERDNLLSKARFEFRWKDQFALSLDPETAES
                     FHDEILPQDRMKEAHFCSMCGPHFCSMKITQDVRDYAESEEAKIGMIEKAKEFREKGS
                     EIYVKTE"
BASE COUNT          644 a          404 c          496 g          526 t
ORIGIN      
        1 tgtggatctg attgtgacat ctccgccttt cctggacaag gtcaattatg ctgcagataa
       61 ctggatgaaa ctctggttcc tcggaattcc tgatgaagag gctgcttccc tcagcattct
      121 gcctacaatt gatgcctggt caaaattcat tcacggcaca ctgcgggaag cgtcgagggt
      181 attgaagaaa aaagcaaaca ttgtgatgga agtcggagat gtggattatc acggaaaact
      241 tgtgaatctg gatgagattg tcattaatat gtcgagagac acagggcttc attggacaag
      301 aacatatatc aattctcaga agtttacaaa actgtccaac tgctggaata tatccaataa
      361 tgaaaaaggg acaaattcga accgctgcgt ggttctgcag aaatcctgaa ttcagtaaaa
      421 aagttttcaa tttctgcccg cttcatatag ttgtaacagc acattgtatt ttacgaggtc
      481 tgtatgaata ttcaggagcc ggcagaatta tctgtacctt ccaaaaaaat cactctcaca
      541 aacggagaga cagtttcatt atattgcaca gagggtcctg aaaatacgga tctgaaaaag
      601 ggtcttccca aaagaagagc agagtggatt cagaaaagaa aggaccgagg tgacagaaat
      661 ttctctcaga tgcactatgc tagaaagggc gagatcaccg aagagatgcg ctttattggc
      721 attcgtgaga acgtcagtcc tgatttcgtc cgggacgaag tcgcaagggg acgtgcgatc
      781 atccctgcaa acatcaatca tacagaactc gaaccgatga tcatcggaag aaatttcctt
      841 gtaaaaatta atgcaaacat cggaaactct gcgctcgctt cttctatcga ggaggaagtg
      901 gaaaaactga aatgggccac taaatggggt gcagacacag tgatggatct ttctacagga
      961 aagaatattc atgaaacaag ggaatggatt cttcgaaatt caccggttcc gataggcact
     1021 gtccctatct atcaggctct cgagaaagtc aagggaaaag cggagaatct gaatatagac
     1081 atctttctgg aaactctgat agagcaggcg gagcagggag tggactattt taccatccat
     1141 gccggcgttc ttctcagata tgttccgatg actgcaaaaa gaactacagg catcgtttcc
     1201 agaggaggat ccattctagc caaatggtgt cttgctcatc ataaggaaaa ttttctgtat
     1261 actcattttg atgatatatg tgaaatcatg aaaaagtatg atatttcatt ctctctcggc
     1321 gatggtctgc gtccaggatg cattgccgat gcaaatgatg aggcacagtt ttcggaactt
     1381 aagaccttag gcgagctgac tcagcgggca tggaaacatg atatacaggt gatgattgaa
     1441 ggaccaggcc atgttcctat gcatatgatt aaagagaata tggaaaagca gctcgagatc
     1501 tgtcatgaag ctcctttcta cactctaggc cctctggtga cagatattgc acccggttat
     1561 gatcatatta cttccggaat cggtgctgca atgatcggat ggttcggaac tgctatgctc
     1621 tgttatgtta ccccgaagga acatctgggg cttccaaaca gggacgatgt aaagactgga
     1681 gtcatcacat acaaaatagc ggctcatgct gctgatctcg caaagggcca tccgggagca
     1741 caggaaagag acaatcttct gagcaaggca agatttgaat tcagatggaa ggatcagttt
     1801 gcactttcac tcgatcctga aactgcagaa agtttccatg atgaaatatt gcctcaggac
     1861 aggatgaaag aagcgcattt ctgttctatg tgcggtcctc atttctgttc aatgaaaatt
     1921 actcaggatg tgagagacta tgcagaatct gaagaagcaa aaatcggaat gattgaaaag
     1981 gcaaaggagt tcagggaaaa gggttctgag atttatgtaa agacagaata gggatcgaag
     2041 tcctttaaaa tctcagggat ctgacactgt
//
LOCUS       DAOQGJ010000647         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36645_length_2069_cov_4.227408, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000647 DAOQGJ010000000
VERSION     DAOQGJ010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36645_length_2069_cov_4.227408"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PK683_10560"
     CDS             complement(<1..1021)
                     /locus_tag="PK683_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08935.1"
                     /translation="MHSLKLKILAVVVLFLAAVRLLDYVLFEKLYFLFPNEMEWDTSP
                     WYNFEHSRKNLKTFSADENGILISGSSVALYSAFPGEIEKKLKSSGMKNPSLHFYSHV
                     AMNPSDFYYYAEDIISKKPKTVIYLLNTGDFQLDYFYFNLKGLQSFLKDPHRPAGEGD
                     EDRIDYDEKRKIAYKKDTYPVKIFYPFEFLKDHLNDLGRQEIFSLLTKTILSVNRVRI
                     YFLDPLTAYYDRHFRKARSYHNYTGAVLKEGIWRKGWTPKEFHIECEADTGILDDSIF
                     IPVPETVLTVKDSDGRELFSRKYEKSGWTDLNIQLKNGKETLHFISGKTVSSRVIDPK
                     SYAREE"
     gene            1020..1313
                     /locus_tag="PK683_10565"
     CDS             1020..1313
                     /locus_tag="PK683_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08936.1"
                     /translation="MTQSINKISRQTVKLFLLLSLSLHAVPKSEEISKPLLRVFPVYR
                     MEECEWAVRMDVCWSCIRKKEKYAQKIYFLKEGGTYREHGCYTEKKGFSVLSE"
     gene            1402..>2069
                     /locus_tag="PK683_10570"
     CDS             1402..>2069
                     /locus_tag="PK683_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH08937.1"
                     /translation="MKIIAEIKSEILQRIRENENLFLFLEDEFTDGYWVFDPENKDTY
                     FNARFCRILGYSDSDTSLNRILSSSELKKLEEFIHGSVLNADSQKNIHQIRYIHASGY
                     SIPFQISIIPLSEVKGRKLLLLANQEIISGKSSADKIKTSAMVEKIPETVNSVFWVRN
                     GERFLYVSPFYESMWGRPRDRLYENFQDFIESIHSDDRQSALDKIRSSILHPERYTIE
                     FRIK"
BASE COUNT          621 a          402 c          404 g          642 t
ORIGIN      
        1 attcttctct tgcataactt ttaggatcaa tgactcttga ggaaactgtt ttgccggaga
       61 tgaaatgaag agtttccttt ccgtttttca gctgtatgtt cagatctgtc cagcctgact
      121 tttcatattt tctgctgaac agttctctcc cgtcactgtc tttgactgta aggacagttt
      181 caggaacagg aatgaatata ctgtcatcca ggatgcctgt atctgcttca cattcaatat
      241 gaaattcctt cggagtccat ccttttctcc agataccttc cttcagcaca gcaccggtat
      301 agttatgata gcttcttgcc tttctgaaat gtctgtcata atatgcagtc agaggatcca
      361 gaaaatagat tctcactctg ttgaccgaaa gaattgtctt tgtaagaagg gaaaatattt
      421 cctgtctgcc aaggtcattg agatgatctt tgagaaactc gaacggataa aatatcttta
      481 ctggataagt atcctttttg tatgcaatct ttcttttttc atcataatcg attctgtcct
      541 cgtctccctc gcctgccggt ctgtgaggat ctttcagaaa actctgaagg cctttcaggt
      601 tgaaataaaa ataatccagc tgaaagtctc ctgtattcag aaggtagatt actgttttcg
      661 gttttttgga tatgatgtct tccgcataat aataaaaatc ggaaggattc attgccacat
      721 gagaataaaa atgcagactg ggatttttca tcccggaaga tttcagtttt ttttctattt
      781 cacctggaaa tgcagagtac agagccactg aacttcctga tataagtatt ccgttttcat
      841 ctgcggaaaa tgtctttaag ttttttctgc tgtgttcgaa attgtaccaa ggagaggtat
      901 cccattccat ttcattcgga aaaaggaaat acagcttttc aaataataca taatcgagca
      961 gtctgacagc tgcaagaaac agaacaacca ctgcaagtat ttttaatttc agagaatgca
     1021 tgactcaatc tataaataaa atctcaaggc aaactgtaaa gctatttctt ctgctttcgc
     1081 tttctcttca cgccgtacct aaatctgaag aaatctccaa gccactcctc cgtgtttttc
     1141 ctgtctacag aatggaggaa tgtgaatggg cagtgcggat ggatgtctgc tggtcctgca
     1201 tcaggaaaaa ggaaaaatat gctcagaaaa tttatttcct gaaggaaggc ggaacataca
     1261 gagaacacgg ctgctacacc gaaaaaaaag gcttttcagt tctttccgaa tagactgatc
     1321 tcagacgttt cagtattgac tctcatttca aaggaataaa actggatgtg agatgtcttt
     1381 tgcaggcgtg ggctttagag aatgaaaatc atagccgaaa taaaatctga aattctgcag
     1441 agaattcggg aaaatgagaa tctttttctt tttcttgaag atgaatttac ggatggatac
     1501 tgggtttttg atccggaaaa taaagataca tattttaatg caagattctg cagaattctg
     1561 ggttattccg attcggatac ttcactgaac agaattctgt cttcatctga acttaagaag
     1621 ctggaagagt tcattcacgg atcagtcttg aatgcagaca gtcagaaaaa cattcatcag
     1681 attcgttata ttcatgcttc aggatattcc atcccttttc aaatcagcat cattcctctg
     1741 tcagaagtca aaggcaggaa actgcttctc ctcgcaaatc aggagatcat ttcagggaaa
     1801 tcatccgccg ataagatcaa aacatcggca atggtggaaa aaattcctga gacagtcaat
     1861 tccgttttct gggtcagaaa cggagaaagg tttttatatg tgagtccttt ttatgaaagt
     1921 atgtgggggc gtccgagaga cagactgtac gagaattttc aggacttcat tgaatccatt
     1981 cactcagatg acagacagtc cgcacttgac aaaatcagat catcgattct gcatcctgaa
     2041 agatatacaa tagagttcag aattaaaaa
//
LOCUS       DAOQGJ010000648         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36686_length_2068_cov_2.868852, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000648 DAOQGJ010000000
VERSION     DAOQGJ010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36686_length_2068_cov_2.868852"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..851
                     /locus_tag="PK683_10575"
     CDS             249..851
                     /locus_tag="PK683_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNH08938.1"
                     /translation="MITISREYGCPGIPISNEVARALTAEKHAEWSVVDKQIVNQAAE
                     ELDVPAKLLEQIAKDKPKGIFEELFMSFSDIHLPTDLKIKKTIARILRTVALHGNVVI
                     LGRGGVVLSRDIQKSLHVHLHASVSWRIGRVKTLENLTSDNEAIGRIKTVDSERVFLK
                     NYFAGETLDSNIYDVSFNCQYLTQDQIVQSILHLAKAKGI"
     gene            complement(894..>2068)
                     /locus_tag="PK683_10580"
     CDS             complement(894..>2068)
                     /locus_tag="PK683_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08939.1"
                     /translation="IETVSETENEVCLKFSLKDTGIGIPEDKLHGLFDPFIQADTSTT
                     RKYGGTGLGLSISKRLAELMGGTIGAESILGEGSVFWFTCTFKKSSSSRNAIPESFPD
                     LKNLKILVTDDHPVNRLVVSSILKKYNCILEEAEGAESAWKKLRESSELGSPFDIVLM
                     DMHMPNMDGLELGKKIQESGIFRSLSMIMLTSLGRRGESAKLQNYGFAGYLTKPVRES
                     NLIDCIALAAGIRKRTDSEPEKEHKIITKYTIKEIRKERTRILLAEDNRVNQLLVLKL
                     LSKMGYRADAVANGAEAVNALKNIPYDAVLMDCQMPEMDGFEATRIIRKKENKVLNPM
                     IPILAITAFAVQGDREKCIECGMDDYLSKPIQPKELKEKLDYWLKRHSVLTEIETV"
BASE COUNT          544 a          447 c          414 g          663 t
ORIGIN      
        1 tggatctctg gctttttgag acatttgact tcgggataaa acatgtgtat tatctcgact
       61 ggcagcttta tctttccaaa atctgctatt gatcctgcat acattaaaaa gtagttgagc
      121 caaatgctgc cgtaatttct cttgaattat caactgatgc aaggtgtttt atgccaagaa
      181 gtccaatact agattatata aaagaaaagg taaatgccgc aaatgaactt agttcagcat
      241 tgccgagtgt gattacaatt tcaagggagt atggctgtcc tggaattcca atttcaaatg
      301 aggttgctcg ggctcttact gcagaaaaac acgctgaatg gtctgtagtg gataagcaga
      361 tcgtcaatca ggcagcagag gaactggatg ttccggcaaa actgctcgag cagattgcaa
      421 aggacaaacc aaagggaatt tttgaagaac tttttatgtc cttttctgat attcatcttc
      481 ctacagactt aaagataaaa aagacaatag ccagaattct tcgtactgtt gctcttcacg
      541 ggaatgtagt cattttggga aggggagggg ttgttctttc cagagatatt cagaaatccc
      601 ttcacgttca tctccatgct tctgtttcct ggagaatcgg cagagtgaaa actctggaaa
      661 atcttacctc agacaatgaa gcaatcggaa gaatcaaaac tgtagacagt gaaagggtct
      721 ttctcaaaaa ttattttgca ggagaaactc tggattcaaa tatttatgat gtcagtttca
      781 actgtcagta tcttactcag gatcagattg ttcagtctat ccttcacctc gcgaaagcaa
      841 aaggaatctg atttccgccc caatccccga agtcttaacg ccttcttcgg ggatcagact
      901 gtttcaattt cagttaggac agagtgtctt ttcagccagt aatccagttt ttccttgagt
      961 tctttaggct gtataggttt tgacagatag tcgtccattc cgcattcaat gcatttttcc
     1021 ctgtcaccct ggactgcgaa tgccgtaata gccagaatcg gaatcatagg attcagcact
     1081 ttattttctt ttttcctgat gattcttgtg gcctcaaaac cgtccatttc aggcatctgg
     1141 cagtccatca gaacagcatc atatgggata ttttttaatg cattgactgc ttctgctccg
     1201 tttgccaccg catctgcacg ataacccatt ttgctcagca gtttaagaac aagaagctga
     1261 ttcaccctgt tgtcttctgc aagaagaatc cttgtccttt cctttctgat ttccttgatg
     1321 gtatattttg taatgatttt gtgttctttt tccggttcgc tgtccgttct ctttctgatt
     1381 ccggcggcaa gtgcaatgca gtctataaga tttgattctc tgacaggttt tgtcagataa
     1441 cctgcaaatc cataattctg aagttttgca ctttcgcctc ttcgccccaa agatgtcagc
     1501 ataatcattg aaagtgatct gaatatgccg ctctcctgaa tcttctttcc cagttcaaga
     1561 ccgtccatat tcggcatatg catatccatc agtacgatgt caaacgggct tcccagttct
     1621 gaactctctc tcagtttttt ccaggcagat tctgcacctt cggcctcttc cagtatgcaa
     1681 ttgtattttt tcagaatgga agaaaccacc agtctgttga ccggatgatc atcggtgaca
     1741 agtattttca aatttttcag atcaggaaat gattctggaa tggcatttct ggagcttgat
     1801 gattttttaa aagtgcatgt gaaccagaaa acagaacctt caccgagaat gctttctgct
     1861 ccgatagttc ctcccatcag ttccgcaagt cttttggaaa tggaaagacc aaggcctgtt
     1921 cctccgtatt ttctggttgt ggatgtatca gcctgtatga agggatcaaa caggccgtga
     1981 agcttgtcct ccggtatgcc tatccctgta tcctttaagg agaatttcag gcagacttca
     2041 ttttctgttt ccgaaacagt ttcaattc
//
LOCUS       DAOQGJ010000649         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36768_length_2065_cov_3.076617, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000649 DAOQGJ010000000
VERSION     DAOQGJ010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36768_length_2065_cov_3.076617"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..1271
                     /locus_tag="PK683_10585"
     CDS             354..1271
                     /locus_tag="PK683_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016749419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08940.1"
                     /translation="MSKAKVLFILFVLFSLNGLFAQAGAPATAAPAKTDAAAEEEAVI
                     KGELIADSDEDDPKASDEEKKRKKDIIKQEAMWRNPDFVGYNKAFEELHKLSKSYSNN
                     KYRLALSSYQSGMNTILKMRENVEQYRKEQAEKKRLNEKWYWQKIDRKAAEERHIQRE
                     KLSAKLAAVTYFTKAIGHMDEVTNPDLREEKAYKDLMSAIYRAWIMAEYDTQNYPQCI
                     PIIELYLEIDNNEKDYPAHKYLASCYSFEENTLKKYKAGSDDQIQKLKNKKNVHLLRA
                     TELKYGKGSVEYKHIVDLVNKDEVISVTP"
     gene            complement(1343..2029)
                     /locus_tag="PK683_10590"
     CDS             complement(1343..2029)
                     /locus_tag="PK683_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH08941.1"
                     /translation="MLDIVRRTHIQVFMSLEIKTFPVYPLGCNCSILFSKEEKKAVVI
                     DPGGDEDKIQKILSDEGLSLERIIHTHAHFDHCMGTSKLASMHSHSKVCMHRDDLGLY
                     KNLPMQCSFFGIHFPAQNITEISHFLEHDEEFLLGKEKIRVIHTPGHTPGSVCFHLEF
                     SGKSLLFSGDTLFAGSIGRTDLWGGDYGKIIDSIKERLFTLEDETLVIPGHGEETRIF
                     EERKFNPFFN"
BASE COUNT          714 a          395 c          388 g          568 t
ORIGIN      
        1 gcatacgaag ggcactgact gcttttcatt tgacggcatt gtcaaaaaag cggtcagaaa
       61 aaggcagact gtgcgaccgc agaatttacg gttcatccga aagctctttt ggggacagtt
      121 ttgaaaattt ttcaaagcag gttaacagga aaagaccgaa attttgagac tcaatttaat
      181 tcaagaaatt ttcatggggc aatcctatag aattatgtct cataaaaaaa aacctctaaa
      241 gtcgaaaaaa agatatttaa tggattgacc tcgtttaggc cttttccgat actatttagc
      301 gagacggtat aacccgtcac caaactattg aaagagagtt aaaggtattg acgatgagta
      361 aagccaaagt attatttatc cttttcgttt tgttcagcct aaatggactt tttgcacaag
      421 ctggagctcc agctacagca gcaccggcaa aaacagatgc ggctgctgaa gaagaggctg
      481 tgattaaagg ggaattaatt gctgacagtg acgaagatga tcctaaagct tctgacgaag
      541 aaaagaaaag aaaaaaggac atcattaagc aggaagcaat gtggagaaat cctgactttg
      601 ttggatacaa taaagctttc gaagagttgc ataaactttc taagtcttat tcaaacaaca
      661 aatacagact ggctctttct tcctaccagt ccggaatgaa cactattctg aagatgagag
      721 aaaatgtaga gcaataccgt aaagaacagg ctgagaaaaa aagactgaac gaaaaatggt
      781 attggcaaaa aatcgataga aaagccgctg aagaaagaca tatccaaaga gaaaaacttt
      841 ctgcaaagct tgctgctgtg acttacttca caaaagctat cggtcatatg gatgaagtaa
      901 ctaaccctga tctgagagaa gaaaaagcat acaaagatct tatgtctgca atttacagag
      961 cttggattat ggcagaatat gacactcaaa actatccaca gtgtatccct ataattgaac
     1021 tctatttgga aattgataac aatgaaaaag attacccagc tcacaaatat cttgctagct
     1081 gctactcttt tgaagaaaat acactgaaaa aatacaaagc tggatcagat gatcaaattc
     1141 agaaactgaa gaacaagaaa aatgttcatc ttctgagagc aactgaactg aagtatggaa
     1201 aaggatctgt tgaatacaag catatagttg accttgtaaa caaagacgaa gttatttctg
     1261 tgactccgta atccacaata cctctttcaa tttaaataaa acagcccttg cgggggttgt
     1321 tttatttaat tgaaatgatt tcttaattga agaaggggtt gaatttcctt tcttcgaaaa
     1381 ttctagtctc ttctccgtgt cccggaatca ctaaagtctc atcctccaaa gtgaacagtc
     1441 tctcctttat tgagtcgata atcttcccat aatcaccgcc ccaaagatcc gttctgccga
     1501 ttgatcctgc aaataaagtg tctccactga atagaagtga ttttcctgaa aattccagat
     1561 ggaaacagac cgagccggga gtatgacctg gtgtatgaat aactctaatc ttctcctttc
     1621 ccagcaggaa ctcctcatca tgttccagaa aatgagaaat ttcagtgata ttctgagcag
     1681 gaaaatgaat tccgaaaaaa ctgcactgca taggaagatt cttataaaga cctaaatcat
     1741 cccgatgcat acagactttg gaatgactgt gcattgaggc cagttttgac gtccccatac
     1801 agtgatcaaa atgcgcgtga gtatgaataa ttctttccag actcaatccc tcatcagaaa
     1861 gaattttctg aattttatcc tcatcgcctc ccggatctat gactacagct tttttttctt
     1921 cttttgagaa cagaatactg cagttgcatc ccaatggata cacaggaaaa gttttgattt
     1981 ctaacgacat aaatacttgg atatgcgtcc ttcgaacaat gtcaagcatc ctatttctac
     2041 taaaaatatc tgctgattta cattt
//
LOCUS       DAOQGJ010000650         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36866_length_2062_cov_2.892377, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000650 DAOQGJ010000000
VERSION     DAOQGJ010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36866_length_2062_cov_2.892377"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /locus_tag="PK683_10595"
     CDS             complement(<1..1844)
                     /locus_tag="PK683_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH08942.1"
                     /translation="MHYHLEIFSPWPDDGVQENASVDKNSRRLYENFITTAMLDVNYM
                     IGSLQGLEISRTKSETLLNMYKINHNIFGFAKILKLNKIFHLASISDFAFDFARKENT
                     LTKHSVDYLIRLLLNMQLKLLKDFMAEDSSSEDISLLIEECRLYLSPPLEAMMEKYRI
                     QTSEFERKGFQTLPPEPENGVRETPSAQEKQAEALPREVPKSVLNEKETDVGLNDFSD
                     EPEELNIPADKIGLISDFYEECSENLGKIGNRLIDLESSPDSSEIVNELFRSVHTVKG
                     GARLLKIRKIELLSHSMENLLDLIRNRKIRFAPELIDYLMDGKSLLSELLEETASKGP
                     LRTRILPVLKKIEVSCRTGSPSKTVSEPIKQELIEVQTKERTEELSSVKSELKTSEAP
                     VSRMSKDKQESIRVSIDKLDEVINSASELSLSRIQFREQIGNVNRVIRDIKRTLSNAQ
                     ENDPGSIIERITKANYVMLSDIRAGLEKYGIHAETEFLNDIVFRFRNELRQELSEREL
                     SVQEELTLLLISFQDVKNAMVKNADNLETLTSKLQNEVMNFRMVPIASLFERFPALIR
                     DMARQTGKKVKMNLNGQDTELDRVMINTLADPLIHVLRNSVDHGIESP"
     gene            complement(1844..>2062)
                     /locus_tag="PK683_10600"
     CDS             complement(1844..>2062)
                     /locus_tag="PK683_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH08943.1"
                     /translation="NQGQEIALCVDSVSKMKEIGKSMIQTNEGLLTGQISEYASGILQ
                     MQKQTLLMISAENLIRSKDIRNYIGDEI"
BASE COUNT          506 a          480 c          440 g          636 t
ORIGIN      
        1 tcgggagatt caattccgtg atcgaccgaa tttctgagta catggatcag cggatcggca
       61 agagtattga tcatcaccct gtccagttca gtgtcctgac cgttcagatt catcttcact
      121 ttcttcccgg tctgtctggc catatcccgg atcagtgcgg ggaatctttc aaacagagat
      181 gcaataggaa ccatcctgaa attcatcact tcattctgaa gctttgaagt cagagtttcc
      241 agattgtctg cattcttgac cattgcattc ttcacatcct gaaaggagat gaggagaaga
      301 gtcagctcct cctgaacgga gagttccctt tcggagagtt cctgtctcag ttcatttctg
      361 aaccggaata caatatcatt cagaaattca gtttctgcat gaattccgta tttttcaagg
      421 cctgcccgaa tgtccgagag catgacatag tttgcttttg taattctttc gatgatgctt
      481 cccggatcgt tttcctgagc gttggagaga gttcttttga tgtcgcggat gaccctgttt
      541 acatttccta tctgttcccg aaactgaatt ctcgaaagtg aaagttcgga tgcggaattg
      601 atcacctcat ccagcttgtc aatgctcact cggatggatt cctgcttgtc cttgctcatc
      661 ctgcttacag gcgcttccga agtcttcagt tcggatttga ctgagcttaa ttcttctgtt
      721 ctttcctttg tctgaacctc aatgagttcc tgttttatag gttcacttac tgtcttggaa
      781 ggagatcctg tcctgcagga aacctctatc ttcttcagaa cgggaagtat tcttgtacgg
      841 agaggcccct tggatgcagt ctcttcgaga agttcggaaa gaagagattt tccgtccatc
      901 agatagtcaa tgagctcagg agcgaagcgg atctttctgt tccgtatcag atccagaagg
      961 ttttccattg aatggctcag aagttcaatc ttcctgatct tcaaaagtct ggctccccct
     1021 ttcacagtat gcactgaacg gaaaagttca tttacaattt cggaagaatc gggagaggat
     1081 tccagatcaa tcagtctgtt tcctatcttt cccaggtttt cactgcactc ttcataaaaa
     1141 tcactgatga ggccaatctt gtcagcaggg atattcagtt cttccggctc atctgaaaaa
     1201 tcattcagtc cgacgtcggt ctctttctcg ttcaggactg actttggaac ttcccgagga
     1261 agcgcctcag cctgcttttc ctgagcagag ggagtttccc tcacaccgtt ctccggctca
     1321 ggaggcaaag tctgaaaacc cttccgttca aattcagatg tctgaattct gtatttctcc
     1381 atcattgctt caagaggagg ggaaagatac agtctgcact cctcaatcag aagggaaata
     1441 tcctcggagc tgctgtcctc tgccataaaa tccttcagaa gcttcagctg catattcaga
     1501 agaaggcgga tgagatagtc gacagagtgt tttgtaagag tattctcctt tctcgcaaaa
     1561 tcaaatgcaa aatcagagat ggatgcgaga tgaaatatct tgttcagctt gagtatcttt
     1621 gcaaatccga atatattatg attgatcttg tacatattca gaagggtttc tgattttgtc
     1681 ctggatattt ccagtccctg aagggagccg atcatatagt ttacgtcaag cattgccgtc
     1741 gtaatgaaat tttcatacag cctcctggaa tttttgtcca ctgaagcatt ttcctgaact
     1801 ccatcgtccg gccatggaga aaatatttct aaatgatagt gcattatatt tcatctccta
     1861 tgtagtttct gatgtccttg gaacggatca gattctctgc agagatcatg aggagagtct
     1921 gcttctgcat ctgaagaatc ccggaagcat actcgctgat ctgaccggtc agaagtccct
     1981 cattcgtctg aatcatggac tttccgatct ccttcatctt ggaaacggaa tccacgcaga
     2041 gagcaatttc ctgaccctga tt
//
LOCUS       DAOQGJ010000651         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36889_length_2061_cov_3.346461, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000651 DAOQGJ010000000
VERSION     DAOQGJ010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36889_length_2061_cov_3.346461"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PK683_10605"
     CDS             <1..974
                     /locus_tag="PK683_10605"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH08944.1"
                     /translation="KLLFTNYKLDSPAEIFSNINREMLELVKQQGYITCFYAVINSEY
                     EVVYSLAGHPRPMLYRSATDEVIQLEGEGTFLGMFEDASQHIKDYRFKLYPGDKLFVY
                     TDGFLEGISDEGDPFQLEDLADLIKKYGQLDTKQCLQKVIQEYHNFCMGTDQSDDITL
                     IAMGLSPKLKEFEDYKSLGEKLYAEGKVGKACESLIKAKEIFPTDMSVLYLLGKYSAK
                     LKDYKSAIEHLEEYNSFKTFNADSHMILGYSYFNMEDYNKAEREFLKSISLRAGNVTS
                     YLYLAKLFFKKKEYDKSRETLNKLLEIEPDHQKALVMMKHLRKLQKS"
     gene            971..1627
                     /locus_tag="PK683_10610"
     CDS             971..1627
                     /locus_tag="PK683_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08945.1"
                     /translation="MIFEKLFRKKKSEPVPLEITDKAVKKIRSQLASRPVGIRSVFKI
                     EVDYRKESYSCSVGFMEKKDEIRTLYSYPIEISISAEDERFLEKFTLEYSDEDSLFYV
                     YPNVEMEIERLPAKNIVRYSLNREIFHPESPLKEIATDKESVPLKIPAFIKKLFQAGA
                     VSVYAKERFFQIEFPDSDITEEQEDIFAEIIKEYYDSCSYPLSAEANQIIPKRQYFSG
                     "
     gene            1710..>2061
                     /locus_tag="PK683_10615"
     CDS             1710..>2061
                     /locus_tag="PK683_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08946.1"
                     /translation="MKPVILCILTAVFLLECSSSVQKISKESVHSAILRETGKKFECK
                     ITDNIVIDNLISLRNRFSSGWKSTWHTLPAAEKEFLFYDRENNLSGQFGFMPGTAVIY
                     EGWLLRSLDKKEEKL"
BASE COUNT          693 a          398 c          412 g          558 t
ORIGIN      
        1 ccaaactgct ttttacaaat tacaaactgg acagtcctgc agagattttt tcaaacatca
       61 accgtgagat gctggaactt gtgaaacagc agggatatat tacctgtttt tatgcagtca
      121 tcaattctga atatgaagtc gtttacagtc tggcaggcca tcctaggccg atgctgtaca
      181 gaagtgccac tgacgaagtc attcagctgg aaggtgaggg tacttttctg ggaatgtttg
      241 aggatgccag tcagcatata aaagactacc gattcaaact gtatcctgga gataaacttt
      301 tcgtttacac ggacggtttt ttggaaggga tttccgatga aggagatcct tttcagctgg
      361 aagacttggc agacctgatc aaaaaatacg gtcagctcga cacgaaacag tgccttcaga
      421 aagtcattca ggaataccac aatttctgta tgggaacaga tcagtcagat gatattacac
      481 tgatcgcgat gggactctct ccaaagctga aggaatttga ggattataaa tccctcggag
      541 aaaaacttta tgcagaaggg aaagtaggaa aagcctgtga atctctcatc aaggcaaagg
      601 aaattttccc taccgatatg agtgttctgt atctgctcgg aaaatattct gcaaaactga
      661 aggactacaa atcagcaatt gaacatctgg aagaatacaa cagtttcaag acattcaacg
      721 cagattcaca tatgatactc ggatacagtt atttcaatat ggaagattac aataaggccg
      781 aaagagaatt tctgaaatcc atatcactca gagccggaaa tgtaacgtcc tacctttact
      841 tggcaaaact ctttttcaag aaaaaggaat acgacaagag cagggaaaca ctgaacaaac
      901 ttctggaaat agaaccggat catcagaagg ctcttgtaat gatgaaacat ctaagaaaac
      961 tgcaaaaatc atgatttttg aaaaactgtt cagaaagaaa aaatctgaac ccgttcctct
     1021 ggaaataacg gacaaagccg tcaaaaaaat cagatctcag cttgcttccc gtcctgtcgg
     1081 aatccgctcc gtattcaaaa ttgaagtgga ttacagaaaa gaaagttatt cctgcagtgt
     1141 aggttttatg gaaaaaaaag atgaaatccg caccctgtat tcctatccta ttgagatttc
     1201 catatcagct gaagatgaaa gattcctgga aaaattcaca ctggaatatt cagacgaaga
     1261 ttcactgttt tatgtttatc cgaatgtgga aatggaaata gaaagacttc ccgcaaagaa
     1321 catcgtccga tacagtctga acagggaaat atttcatcca gaatctccgc tgaaagaaat
     1381 cgcgacagac aaggaatccg ttcctctgaa aatccctgct tttataaaaa agttatttca
     1441 ggcaggtgcc gtttctgtat atgcaaagga acgttttttc caaatcgaat ttccggattc
     1501 ggacatcacg gaagaacagg aagatatttt tgctgaaatc attaaggaat attatgattc
     1561 ctgctcttac cccctgtctg ctgaagcaaa tcagatcatc ccgaaaagac agtatttttc
     1621 gggttgacat tctgttttaa aactgcagtc tgaaactatg tctgtcgcaa ccgtaaaact
     1681 gactgtcact acatggaatc tgatatttaa tgaaaccagt aattctctgt attctgactg
     1741 cagtattttt gctggaatgc agttcttctg ttcagaaaat ttccaaggaa tctgtccatt
     1801 ctgcaatcct cagagaaaca ggcaaaaaat tcgaatgcaa aataacagat aatattgtaa
     1861 ttgataatct aatctcactg agaaatcgtt tcagttccgg atggaaaagc acctggcata
     1921 ctcttcccgc agcggaaaag gaatttcttt tttacgacag agaaaataat ctttcaggtc
     1981 agttcggttt tatgcccgga actgctgtaa tatatgaagg atggcttctt cgaagtctgg
     2041 ataaaaaaga agaaaaactg c
//
LOCUS       DAOQGJ010000652         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_36957_length_2059_cov_3.562874, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000652 DAOQGJ010000000
VERSION     DAOQGJ010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36957_length_2059_cov_3.562874"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PK683_10620"
     CDS             <1..339
                     /locus_tag="PK683_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNH08947.1"
                     /translation="YMKILPEFIVNEYVHRIINIHPSLLPAFPGINSIHKAYSYGSQI
                     SGCTTHFVDNTVDGGPIILQSAVKIKNGMSETDLTEVIHKEEHKILPLSVQYFIEDRI
                     QIEGRRITIL"
     gene            342..1883
                     /gene="purH"
                     /locus_tag="PK683_10625"
     CDS             342..1883
                     /gene="purH"
                     /locus_tag="PK683_10625"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNH08948.1"
                     /translation="MIQIKRALISVSDKTGLLEFAKFLNSRKVEILSTGGTLKALKDG
                     GIPAIAVDQYTGFPEMLEGRVKTLHPKIHGGLLADLDNPLHKSEAEKHGIPSIDLVVV
                     NLYPFAETVSKAGVTLEQAIENIDIGGPSMLRSGAKNNKHTVVVTEISDYQLIQSEME
                     KNDGKVSREISFELARKVYSVTASYDATIANYLNDLKSDKFPEKLTLTYKKKQGLRYG
                     ENPHQKAAFYEPVLIKNEFTPLQGKELSFNNMLDFDAAFHIASLLPENAVSIIKHLNP
                     CGIASAENPLDSFLLARRTDPISAFGGIIGINGEVGKDLANAITENFVEGVLAKKFTS
                     EAREFFAKKQNIRLIEIPNYKEALNEMDVRPIHHGILLQDMDFESIKKENLKVVTKKK
                     PSEEDMEGLLFAWNVVKFIKSNAIVYTDMNSTLGIGAGQMSRVDSVELGALKAQKSML
                     SVVGSYVGSDAFFPFRDGIDAIAKVGAKAIIQPGGSIRDEEVIQAADEHGLIMVFTGI
                     RHFRH"
BASE COUNT          634 a          386 c          458 g          581 t
ORIGIN      
        1 tatatgaaga tacttccaga atttatagta aatgaatacg ttcacagaat tatcaatatt
       61 cacccgtcac tcctgcctgc atttcccgga atcaattcaa tccataaggc atattcttat
      121 ggttcgcaaa tttcagggtg tacgactcat tttgtcgata atacagtaga cggcgggccg
      181 atcatccttc agtctgcggt aaaaataaag aatggaatgt cagaaactga tttgacagaa
      241 gttatacata aagaagaaca taaaatcctg cccttgtcag tgcagtattt tattgaagat
      301 agaattcaga tagaaggaag aaggattaca attttatgag tatgatacag ataaaaaggg
      361 cattgatttc agtaagtgac aaaacaggtc tcttggagtt tgccaaattt ctgaattcca
      421 gaaaagtgga gattctctct accggaggaa ccctgaaagc actgaaggac ggaggaattc
      481 cagcgatagc tgtggatcag tataccggtt ttcctgaaat gctggaagga agagtaaaga
      541 cccttcatcc taagattcac ggaggccttt tggccgattt ggacaatcct cttcataaat
      601 ctgaggcaga aaagcacgga attccttcta ttgatctggt ggtcgtgaac ctgtaccctt
      661 ttgctgaaac tgtcagcaag gcgggagtga ctttggaaca agcgattgag aatatcgata
      721 tcggcggacc ttccatgctc agaagcggcg caaagaataa taagcatact gttgttgtca
      781 ctgaaatttc tgactatcag ctgattcagt ctgaaatgga aaaaaatgac ggaaaagttt
      841 ccagagaaat ctcatttgag cttgccagaa aggtttactc ggtgaccgca tcctatgatg
      901 caacaattgc aaattatctg aatgatctga aatcagacaa attcccggaa aaactgacat
      961 tgacttataa gaaaaaacaa gggctgagat atggtgagaa tcctcatcag aaagcagctt
     1021 tctatgaacc ggtcctgatc aaaaatgagt ttacaccgct ccagggcaaa gaactttcct
     1081 tcaataatat gctggacttt gatgcagctt tccatattgc atcacttctt cctgaaaatg
     1141 cagtctccat cattaagcat ctcaatccct gcggtattgc ttctgcagaa aatcctcttg
     1201 attcatttct tctggcaaga agaacagatc cgatttctgc tttcggcgga atcattggta
     1261 tcaatggaga agtcggaaaa gatctggcaa atgcgattac tgaaaacttt gtagagggtg
     1321 ttcttgctaa gaaattcact tcggaagcca gagagttttt tgcgaaaaag cagaatatca
     1381 gactgataga aattccaaat tacaaggaag ctttgaatga aatggatgtt cgtcctattc
     1441 accatggaat tctgcttcag gatatggatt ttgagagtat aaagaaagaa aatctgaaag
     1501 tagtcacaaa gaaaaaacct tccgaggaag atatggaagg tctccttttt gcatggaatg
     1561 tcgtcaaatt cattaagtca aatgcaattg tctacacgga tatgaattct actttaggaa
     1621 tcggtgctgg tcagatgtcc cgggtcgatt cagttgaact gggtgcactg aaagcccaga
     1681 aatccatgct ttccgttgta ggcagctatg taggaagtga tgcatttttt ccttttcggg
     1741 acggtatcga tgcgattgcg aaagtaggag caaaggcaat cattcagccg ggcggatcaa
     1801 tcagggatga ggaagtcatt caggctgccg atgaacacgg tctaattatg gtgtttaccg
     1861 gaattcgtca tttcaggcat taaaaaggag tcagtatgga gttcttgctt ttcctgtttt
     1921 tttatctgct gtctttggct gtactgcccc tctgtgccta ctattatctt ctgttcagcg
     1981 attccgaaag ggaaagcttc agccgttctg aactgattga tatgaagaaa atcattccta
     2041 gagagcttat aggagtgat
//
LOCUS       DAOQGJ010000653         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37025_length_2057_cov_2.873626, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000653 DAOQGJ010000000
VERSION     DAOQGJ010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37025_length_2057_cov_2.873626"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="PK683_10630"
     CDS             complement(<1..1511)
                     /locus_tag="PK683_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH08949.1"
                     /translation="MKLTLQILGVVYPLLWVLKPQEVTGPGEFAERFLFGIIAFIISF
                     SIESKSESVRKLSVPFGLIFLFSVTYHSIYRCYTSSYSLDYTASAYLVFFITISSITD
                     AKILFFYAFFSVAGNLIASSLILPKTGFYQFFFPTLLAFAGGTVLMNRKISDKNFLLL
                     SEEFIQKNLDREDAGIFLTDSEEKIQFINQNAKNIIKSGFRDDFILGSRIILPVPPEA
                     RKLGIPFRFMIEGDIHFEARYVKAEWKGHPCFLVILKDVTEEERRNREKEKQRIINEN
                     VLKHTGEGIIFLNTEGFLEYINPEGCGLLGMEREELVGEFFLAKVHHTDLAGNTYEEE
                     NFPPNVTYTEGKPFHISFDIFWRKDGSHFYTDYTSTPVFINEKLEGAILVFRDVTERK
                     EKEDAEKKYTDDIMHLSLTSNRFLEIYTEPELYRFIADEAGEIAKSAVIVNVFDEETG
                     RFTTKATRGFEKLQNELHLLLGRDSKDLEYFLDSSDPALELNQRDAFLPLSEGL"
     gene            complement(1508..>2057)
                     /locus_tag="PK683_10635"
     CDS             complement(1508..>2057)
                     /locus_tag="PK683_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH08950.1"
                     /translation="LPEAVGGIVLLFGRILQFDKPIITEVTGHAMGGGAVITTAGDYK
                     YMLNSGCRIGFTEVMVGLPLPGMFVTRVMELVPPGKVREVCLEASTYKGKEAKEVGLI
                     DETADDREGLRKLTMKKLETLLRLPMSGVRYTKQNINRKSLFEFEAHMKNSLDWMHIP
                     VVSENLLEAMRALKEKRRPNLK"
BASE COUNT          552 a          443 c          421 g          641 t
ORIGIN      
        1 agtccttccg aaagaggaag aaatgcatct ctctgattca gctccagggc aggatctgag
       61 ctgtcgagaa aatactcaag atctttcgaa tcccttccaa gaagaaggtg aagttcattc
      121 tgcagttttt caaatccgcg tgttgcctta gttgtaaatc ttccagtttc ctcatcgaag
      181 acatttacaa tgactgctga ttttgcaatc tctcctgctt cgtctgcaat gaaccgatac
      241 agttcaggtt cagtataaat ctcaaggaat ctgtttgagg tgagcgaaag atgcataata
      301 tcatcagtat atttcttttc tgcatcctcc ttttcctttc tttcggtgac atctctgaac
      361 acaagtattg caccttccag cttttcgttg ataaagacag gagttgaggt gtaatcggta
      421 taaaaatgac tcccgtcctt tctccagaaa atgtcaaagc tgatatggaa aggcttgcct
      481 tcagtgtatg tgacatttgg aggaaaattt tcctcttcgt aagtattgcc tgcaagatcc
      541 gtatgatgaa cttttgcgag gaaaaactct ccgaccagtt cttctctttc cattccgaga
      601 agtccgcagc cttcagggtt gatgtattcc aggaaacctt cagtgttcag aaaaatgatt
      661 ccttcgcctg tatgtttcag aacattttca ttgatgattc tctgtttttc tttttcgcga
      721 tttcttcttt cttcttcagt tacgtccttg agtataacca gaaaacaggg atgtcctttc
      781 cattctgcct ttacatatct ggcttcaaaa tgaatatctc cttcaatcat gaaacggaat
      841 gggattccga gttttctggc ttccggagga acaggaagaa tgattctact gccgagaata
      901 aagtcatccc tgaatccaga tttaataatg ttctttgcat tctgattaat gaactgaatt
      961 ttttcttctg agtcagtgag aaatattccc gcatcttcgc gatccaggtt cttttgtata
     1021 aattcttcgc tgagaaggag aaaattcttg tccgaaattt tcctgttcat caggactgtg
     1081 ccgccggcaa aggcaagaag agtaggaaaa aaaaactgat agaatccggt tttgggaaga
     1141 atcagtgagg atgcgatcag gtttccagca acggaaaaaa atgcatagaa gaacagaatc
     1201 tttgcatcag tgatgctcga aattgtaatg aaaaagacca gataggctga ggcggtgtag
     1261 tcaagactgt atgaacttgt ataacatctg tatatgctgt ggtacgtgac ggaaaaaaga
     1321 aaaatcagac cgaaaggcac actgagtttt cggactgatt ctgactttga ttcgattgaa
     1381 aaagaaataa tgaaagcgat aatgccgaaa agaaatcttt ctgcaaattc tcccgggcct
     1441 gtgacttcct gaggctttaa aacccagaga aggggataca caactcccag tatctgcaga
     1501 gtcagtttca tttcagattt ggacgccttt tttctttcag tgctctcatt gcttccagaa
     1561 gattttcgct gactaccgga atatgcatcc agtcgagtga gtttttcata tgtgcttcaa
     1621 attcaaaaag ggatttcctg ttgatattct gcttcgtata tctgactccg ctcataggca
     1681 gtctcagcag tgtttccagt tttttcattg tcagttttct gagtccttcc ctgtcgtcgg
     1741 ctgtttcgtc aatcagaccg acttcttttg cttcttttcc cttgtatgtg gaagcttcca
     1801 gacacacttc tctgacttta ccgggaggaa caagttccat tacacgtgtc acgaacattc
     1861 cgggaagagg gagtccgacc attacctcag taaatccgat tctgcatccg ctgttcagca
     1921 tatatttata gtctccagct gtagtaatga ctgctccgcc tcccattgca tggcctgtca
     1981 cttcggtgat aataggtttg tcaaactgca gaattcttcc gaagagaaga actatcccgc
     2041 ccactgcctc aggcagt
//
LOCUS       DAOQGJ010000654         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37123_length_2054_cov_2.984992, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000654 DAOQGJ010000000
VERSION     DAOQGJ010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37123_length_2054_cov_2.984992"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..305
                     /locus_tag="PK683_10640"
     CDS             90..305
                     /locus_tag="PK683_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08951.1"
                     /translation="MTSASPAFSSAGSPPVNTIGLMKLTANRGTAIRKYNIIGPAYGY
                     RGKSVPKKANIYFKKKQGNRDITMAGR"
     gene            complement(373..>2054)
                     /locus_tag="PK683_10645"
     CDS             complement(373..>2054)
                     /locus_tag="PK683_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08952.1"
                     /translation="VEKEAFKIGEVIQREIKKSDFKIDQYVKEKSYELDQVNQRYTKE
                     VDDLNRNLNGIQVKGSELLESFKEEFEKGRTVLDNLFHSYEDQFKERSETLFADMTLK
                     LRKTTDEIESEIGRLQKSGAGILEKHETSLAKYTEKLEMDIQSKLEKIKFESEEVLDQ
                     IQKTGHNLLEKQEEKIDKFNTTLDDKISRQLTILIDKGQLQLGALEARIANYINDVRG
                     NIEESLKKAKEDSDGQISDFNEHVKMSLKEIENTSTEFLNSSAREFTRSKDEFNKLKD
                     SIRQESSRLEEMKENLYNFLAEENSKIQVQRSRSDQMKEELNKVSDSLFTIQRKTAEL
                     QDKITMLTSIDEKIILLQKVYKEVEEKSENLESVNERIGELLRSMEDTEKSESFLRTG
                     IRQLMTEVNLLQDRENEILDNLSQIESKTAFLQSRSSDIKSIESKFEKVEGMMMDLSS
                     KHKQISALQNRLQSLHEESEEMKHSMENLLKEVDDKFDKLSSFASAVDSVSSGKSKGN
                     GRETGGEKMKKKRAIVMELYEAFDWSPETISEKLNMEKSLVETIINSRKNS"
BASE COUNT          518 a          459 c          361 g          716 t
ORIGIN      
        1 aggagaaaga atttcattat gcaaatttcc agtacgtcca atagattccg ctgaacagaa
       61 aaatcccaat acaggcccct tgaaaataga tgaccagtgc atctcctgca ttttcatctg
      121 caggaagtcc ccctgtaaac actatcggac ttatgaaact gactgcaaac agagggacag
      181 caatcagaaa atataatata atcggccctg catacggata tcgcggaaag tctgtgccca
      241 aaaaagcgaa tatatacttc aaaaaaaagc agggaaacag ggatatcaca atggcaggaa
      301 gataaaatat tacgaacata agcatttctc tgaataacag gaaatccttt caccgttttt
      361 ttcaatcatt aattatgaat ttttacggct gtttataatt gtttccacta gggacttttc
      421 catattcagc ttctctgaaa ttgtttcggg agaccagtca aatgcttcat aaagttccat
      481 gacaatagct ctttttttct tcattttttc tcctccagtt tctcttccgt ttcctttcga
      541 tttgccggat gatacactgt ccactgcaga tgcaaaactg gaaagcttgt caaacttgtc
      601 atccacttcc ttcagaagat tttccatgct gtgcttcatt tcttccgatt cttcatggag
      661 ggactgcaga cggttctgaa gagccgaaat ctgcttgtgc ttggaggaaa gatccatcat
      721 catcccctcc actttttcaa acttggattc gatggacttg atgtccgaac ttctggactg
      781 aaggaaggct gtcttggatt ctatctgaga cagattatca aggatctcat tttctctgtc
      841 ttgaagaaga ttgacctccg tcatcagctg acgtatccct gtacgcagga aggactcgct
      901 tttttcagtg tcttccatgc ttcttaaaag ctcaccgatc ctttcattga cagattcaag
      961 attttcggat ttttcttcca cttccttgta tactttctga agcaggatga ttttctcatc
     1021 aatggaagtg agcattgtaa tcttgtcctg aagctctgca gtttttctct gtattgtaaa
     1081 cagtgaatcg ctgaccttgt tcagctcttc cttcatctga tccgatcttg atctctgaac
     1141 ctgaattttt gaattttctt ctgcaaggaa attatacaga ttctccttca tctcttcaag
     1201 tcttgaactt tcctgacgga tggaatcctt cagtttattg aattcatcct ttgatctggt
     1261 aaattctctt gcagaagaat tcagaaattc agtgcttgtg ttttcaattt ccttcagact
     1321 catttttaca tgttcattga aatcggagat ctggccgtca ctgtcctctt tggccttctt
     1381 caggctttct tcaatatttc ctctgacatc attgatatag tttgcaattc tcgcttccag
     1441 ggcacccagc tgaagctgac ccttgtcgat aagaatagtg agctgtctgg atatcttgtc
     1501 atccagagtg gtattgaatt tgtcaatttt ctcttcctgt ttttcaagaa gattgtggcc
     1561 tgtcttctgt atctgatcca gaacttcttc agactcaaat ttgatctttt cgagtttgga
     1621 ctgaatgtcc atttccagtt tctctgtgta ttttgcgaga gatgtttcat gcttttccag
     1681 aatcccagca cctgatttct gaagtcttcc gatttcagat tcaatttcat ccgttgtctt
     1741 tctgagcttg agagtcatat cggcaaaaag agtctcggat ctttccttga actgatcttc
     1801 gtaactgtga aaaagattgt cgagaactgt ccgtcctttt tcaaattctt ccttgaagct
     1861 ttcaagaagt tcagaaccct tcacctgaat tccgttcaga ttcctgttca gatcatccac
     1921 ttctttagta tatctctgat tcacctgatc gagctcatag gatttttcct tcacgtactg
     1981 atcaatcttg aagtccgatt ttttgatttc tctctgaatg acttcaccga ttttgaaagc
     2041 ttctttttcc acct
//
LOCUS       DAOQGJ010000655         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37126_length_2054_cov_2.855928, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000655 DAOQGJ010000000
VERSION     DAOQGJ010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37126_length_2054_cov_2.855928"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            842..964
                     /locus_tag="PK683_10650"
     CDS             842..964
                     /locus_tag="PK683_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08953.1"
                     /translation="MEPIETLCTEKEELIGIVLNGTGEGYQYTCLISEGDEAKN"
     gene            1163..1960
                     /locus_tag="PK683_10655"
     CDS             1163..1960
                     /locus_tag="PK683_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08954.1"
                     /translation="MRSIEKYFGRVLFYVTAVIATHMSCTSRISESSAVRESKVDASI
                     SVPDPVLLPSSELIPYARLGATEAYKIQGFEVEWVKFGSDPHKQDCLKALQTALFQIK
                     TAVPPEAYKILKSKLILFSEANTDDPAANDAFYYGNRVQITRRKEYLKRPLYITFHEL
                     AHAYDDGFVRIHKLRKEEAYESAMYRKIYGTSKTGVNEYDFKNYLTSNPEEYFAELSC
                     AYFIGLKYFPYNRETLKEKDPNGYRLMEDAWVYQKSPNAFPDVEVEP"
BASE COUNT          635 a          375 c          394 g          650 t
ORIGIN      
        1 aaggactagg ttgttactct ttctgcaggt cgtgctgctt tctttactct cttagctctc
       61 tcaattctct cagttccttt caaacataaa tcttgataat cgcaaatctt tccactttca
      121 atcttcaatt cgtctgattc atctgattga aatttctaaa atctaaaatc cttgatcagc
      181 aaaatccata taataatggt ttttagagcc ttaaaaatga aatactatgt atttgcaggc
      241 ggatcttgaa tgtattcaga tttcatttac aagatcattt gatttgtgca aaaatgaata
      301 ttcattgtca aaaactcaca ttttttttta ctgaaatgag tttcgagaaa atgatcgctc
      361 taaataaaag atttttcgtt cttattactc ttttgcaatg tatctctttc tgttcagttt
      421 cggttgaaaa gaaagacgac tggagtactt tcactgtata caatgtgcgg aatgactacg
      481 tacagagaat tcgttatgtc aagacatttt atctgaataa ttccattttc ggactcattt
      541 cgaaaaagga gctgagagag gaggatctct gtgacggaga tgagatagaa catataaagt
      601 tttctcagtt ttcagcgatt tcatgtgctt tatcgtttct gatttattgc ccagtttatc
      661 ttggagtcgt gtgccgaaaa tgagtattct tatttatatc tctctcttat tcctgcctta
      721 tttattacaa caagggacac ataaaattca gacagttacg gttcatctga aaggatcaga
      781 aaaaaaagga aaaagagaag ttcgttttaa tccagtaaat tggcgtgttc atcaaaaaca
      841 gatggaacca atagagacgc tgtgtacaga gaaagaagaa ctcatcggga tcgtactgaa
      901 cggaacaggt gagggatatc agtatacctg ccttatctcc gaaggggatg aagcgaaaaa
      961 ttagatattc gatgagttta attctgtctt cgcttatctg tcgctttttc gaaaaaatct
     1021 tgcaaattcc ttttctctga tcagtcttgt gctgataaat attatactga tcaccgacaa
     1081 taacagtgct tttttgggtt cactcaacag gatttgacag gcaaaaattt ttgagtatca
     1141 gtataactga aggagaagcg gtttgagaag catagaaaaa tatttcggca gagtcctatt
     1201 ttatgtcaca gctgttattg cgacccatat gtcttgcacc tcacgaattt ctgaatcctc
     1261 tgctgtcaga gaatccaaag tggatgcttc catttctgtt cctgatccag ttttacttcc
     1321 gagctctgaa ctcattccct atgctcgact tggagcgaca gaggcttata agattcaggg
     1381 atttgaagtg gaatgggtca agttcgggag tgatcctcat aagcaggatt gtctcaaggc
     1441 tcttcagact gcactttttc agataaagac tgccgttccg ccagaggcat ataaaatatt
     1501 gaaaagcaag ctgattctct tcagtgaggc caatactgac gatcctgctg caaatgacgc
     1561 attttactac ggcaatagag ttcagattac cagacggaag gaatacctga aaagaccttt
     1621 gtacattaca tttcatgaac ttgcccatgc ctatgatgac gggtttgtaa gaatacataa
     1681 gctgagaaag gaagaggctt atgaatctgc aatgtacaga aaaatctacg gtacttctaa
     1741 aacaggagtc aatgaatatg atttcaaaaa ctatcttaca tccaaccctg aagaatattt
     1801 tgcagagctt tcctgtgctt attttattgg actgaaatat ttcccttaca accgtgaaac
     1861 actgaaggaa aaggatccta acggatacag gctgatggaa gatgcctggg tatatcagaa
     1921 atctccgaat gcatttccag atgtggaagt ggaaccgtaa accgaaagca aattctgcaa
     1981 ataataattc attttataat ttcagatttt gaaagcaaga ctcagttttc tcagttattc
     2041 cgcagagagt accg
//
LOCUS       DAOQGJ010000656         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37287_length_2048_cov_3.158053, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000656 DAOQGJ010000000
VERSION     DAOQGJ010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37287_length_2048_cov_3.158053"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2048
                     /locus_tag="PK683_10660"
     CDS             <1..>2048
                     /locus_tag="PK683_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH08955.1"
                     /translation="VTEPIERAVWGLDAVEYVYSASSAHGAVITVRFKVGEPTEPSLV
                     KVHHKLMEIKQDLPQNVMNPKVSSFTIDDVPFLTLTFSSENRDDYELRKLIAPLAREL
                     SSTPDLARVELLGGRKETVRVIIDPAKAAGFGVSIADAAMSLKMSDAYFPVGKTFSQD
                     REFQVEVGGRMSSAADIGNIAIGQRGGAVVRMKDIAKIELGPEERSRLSVLYDKELGG
                     TQRKAVSVVFSKRKGTNVVVLAKDLLERAGTFSSEIPSDIKMSVVRNYGTTAEEKSFE
                     LIEHLLIATFSVAVLIAVFMGLRASIVVSIAIPVTLALTLAIYYFMGYTLNRVTLFAL
                     IFSIGILVDDAIVVVENVERHLHLHPERGILRATIDAVSEVGNPTILATFTVIGAILP
                     MAFVRGLMGPYMKPIPVGASMAMILSLFVAFIVTPWASVRLLKHSPKHSEENHESDAD
                     PLDKSKVSFLDRLYISFAEWLLSGTKSALIFGAVSIVLLLGSFGFIYFKAVKVKMLPF
                     DNKNEFQIIIDYPSDTPLMKSVEMTKELAVQLSKHPEVKKIQIYGGEAAPFSFSGMVK
                     HTFLRNREYMNDLQILLSDKDHRKESSHKIIESLRPVIAEFGKSKKAVTKVLEIPPGP
                     PVMATMVAEVYGPSEEVRRKVAGEVFTIFQSERSVVDLDYSWREGKTRKIYEYDQSKG
                     "
BASE COUNT          549 a          396 c          518 g          585 t
ORIGIN      
        1 ggtgacagag ccgatagaaa gagctgtctg gggactggat gctgtggagt atgtgtactc
       61 tgcaagttct gctcacggtg cagtaattac tgtccgtttc aaggtgggtg aaccgactga
      121 accttctcta gtcaaggtgc atcataaact gatggagatc aagcaggatc ttcctcagaa
      181 tgtaatgaat cctaaagtaa gttcttttac tatagatgat gttccttttc tgacacttac
      241 tttcagttct gaaaacagag atgattatga attgagaaag ctgattgcac ctcttgccag
      301 agaactttcc agcactcctg atcttgccag agttgaactt ctgggaggaa gaaaggagac
      361 tgtcagggtg attattgatc ctgcaaaggc ggcaggtttc ggagtctcga ttgccgatgc
      421 tgcgatgagt ctgaaaatga gcgatgccta ttttcctgtc ggaaagactt tttcacagga
      481 cagggaattt caggtggaag taggcgggcg aatgtcctct gctgcagata tcggaaacat
      541 tgcaatcggt caaagaggcg gagcggttgt ccggatgaag gacatcgcga agattgaact
      601 tggtcctgaa gaaaggagca gactgtctgt tctttatgac aaggagctgg gaggaactca
      661 gagaaaggct gtatcggtgg ttttttccaa aagaaagggg accaacgtcg ttgtgcttgc
      721 caaggatctt ctcgaacggg caggcacatt ttcttccgag attccttccg acattaaaat
      781 gtctgtggtt cggaactacg gtactactgc tgaagagaaa tcatttgaac tcattgaaca
      841 tcttctgatc gccactttct ctgtggctgt tctgatcgct gtgtttatgg gactgagagc
      901 ttccatcgtg gtctcaattg cgattccggt cacactggct ttgactctgg ccatatatta
      961 ctttatgggt tatacattaa atcgtgtgac tttatttgcg cttatatttt ctataggtat
     1021 tctggtggat gatgcgattg tggtcgtgga aaacgtggag cggcatctgc atcttcatcc
     1081 agaaagagga attcttagag caactataga tgcagtgtct gaagtgggaa atccgacgat
     1141 actggctaca tttacagtca tcggcgcaat tctcccgatg gcatttgtaa gaggactgat
     1201 gggaccttat atgaagccga ttcctgttgg tgcaagtatg gcgatgattc tttccctgtt
     1261 tgttgctttc attgttacgc cttgggcatc tgtccgtctg cttaaacaca gtccaaaaca
     1321 ctcagaggaa aaccatgaaa gtgatgcaga tccactggac aagtcaaagg tttcttttct
     1381 ggacaggctc tatatttctt ttgcagagtg gcttctcagc ggaacgaaat ctgcactgat
     1441 attcggggct gtcagtattg tgcttctgct cggttcgttc ggctttattt actttaaggc
     1501 tgtgaaagtg aagatgcttc cgtttgacaa taaaaatgaa tttcagatca ttatagatta
     1561 tccttccgac acccctctta tgaagagtgt ggaaatgaca aaggaacttg cagttcagct
     1621 ttcaaaacat cctgaagtaa aaaagattca gatatacgga ggagaggctg ctcctttttc
     1681 attctcagga atggtcaagc atactttcct tcggaacagg gagtatatga atgatctgca
     1741 gatccttctt tcggacaagg atcacagaaa agaatccagt cataaaatta ttgaaagtct
     1801 gcgtcctgtg attgcagaat tcggaaagtc caaaaaagca gtgacaaagg ttctcgaaat
     1861 acctccaggg cctccagtaa tggctacaat ggtagctgag gtctatggtc cttccgaaga
     1921 agtgagacgc aaggtggcag gtgaggtatt cacgattttt caaagtgaac gcagtgttgt
     1981 cgatctggac tattcctgga gagagggcaa gacaagaaaa atttatgaat atgatcagtc
     2041 caagggag
//
LOCUS       DAOQGJ010000657         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37319_length_2047_cov_3.835843, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000657 DAOQGJ010000000
VERSION     DAOQGJ010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37319_length_2047_cov_3.835843"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PK683_10665"
     CDS             complement(<1..679)
                     /locus_tag="PK683_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08956.1"
                     /translation="MILKKKNEVPVLSFFVFLVSAAVSLLVVYLISEHENQSLHDIVR
                     SNQKNIIIIFGNQLDSRKKTLKRIARRWENRKRTPVSDWNADAAEYLKDFPEILSFKW
                     IDSSYRIGLRYPELQTTETESIISNSELLKALQASRSSGNSAFSYAFYRYSESKEICF
                     TAFFPVFIENRFDGFIAADFDLKFLLYSIMEKARITDFYIRIYENSKQIFSSVPEKYT
                     SEYSSEKK"
     gene            complement(1061..>2047)
                     /locus_tag="PK683_10670"
     CDS             complement(1061..>2047)
                     /locus_tag="PK683_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08957.1"
                     /translation="GIFTAMACVSQKSKNSLPAKGEKYDLVQEMNSITAPSGLVFKAA
                     TMERDKTDTESRPVFFTKTQIAKKITKLDNTTSSIVFNLILKHTPASVDDYSNYKGVF
                     LIEGQEPDYRSGGGLSTVRRGLSVVYQKSGSTLKYKMMNALYNSAMLPEQMFTPSLEL
                     NPKPASGPTAADGWANDFNVMISSIDDSGKGSVAYGWQANASDGYLRTFNVETKSDDT
                     GTAYFGFADNNTSGTGDLKLDRMICNWAGPAGNRTGQQKVQRQTIKYDIRFNLFVPVT
                     DNITFAPTNSCNWNFGTNPTTQFQGVTTQGITNNLIDMSAYSFSLPESPAAP"
BASE COUNT          541 a          424 c          421 g          661 t
ORIGIN      
        1 gttttttttc cgaagagtat tccgaagtat atttttcagg aaccgaactg aatatctgtt
       61 tcgagttttc atatattctg atataaaaat cagttatcct tgccttttcc ataattgaat
      121 aaagcagaaa tttcagatca aaatcggcgg caatgaaacc gtcgaatctg ttttcaatga
      181 agacagggaa aaatgcagtg aagcagattt ccttggattc ggagtatctg taaaatgcgt
      241 aggagaaggc agagttccct gagcttctgg atgcctgcaa tgctttcaga agttcagaat
      301 tggaaatgat gctttcggtt tccgttgtct gcagttccgg atatcggagg ccgattctgt
      361 aactgctgtc tatccatttg aaagaaagta tttcaggaaa atccttcaga tactctgccg
      421 catctgcatt ccagtctgaa acaggagttc tttttctgtt ttcccatctt ctggcaattc
      481 gcttcagtgt cttttttctg gaatcgagct gattgccgaa tatgataatg atgttcttct
      541 ggtttgatct tacaatatca tgaagactct gattttcatg ttccgatatc agatacacta
      601 ctagcagact tactgcggct gaaacaagga agacaaaaaa agacagaaca ggtacttcgt
      661 ttttcttttt caggatcata ttatacagtg aagcggggac tgaaatgaaa ctctctaaaa
      721 atcaaatgag acaatgttta cactccttcc agttctttat ataaagaaat ttctgccaac
      781 ctaaagcctg ccgacgctgc atccagctta aaaaacctgc tttccttcac tttagggctt
      841 gctgaccagc tgcagttccc ggcaaggaat gcatttatag tctctcaatg caatatatta
      901 tacattctta atataacaat acaatacatt tttcagcgta tatcaaaaaa agttattctt
      961 tctttagctg cttcagattt ttgcgtaggg gttttctttc agagatacat cagtcctgtc
     1021 aattttgaca ggactgatgt atcggaaact gtatgatttt ttacggagcg gcgggagatt
     1081 caggaagaga aaaagaatat gcggacatat caatcagatt atttgtaatt ccctgagtcg
     1141 tcactccttg aaactgagtc gtaggatttg ttccaaagtt ccagttgcag gaatttgtag
     1201 gtgcaaatgt aatattgtcc gttacaggaa caaacaggtt gaatctgata tcatacttga
     1261 ttgtctgtct ctgcactttc tgctgccctg ttctgtttcc tgcgggtcct gcccagttgc
     1321 agatcattct gtccagtttg agatccccgg ttccggatgt gttgttgtcc gcaaatccga
     1381 aataggcagt gcctgtatca tcggattttg tttctacatt gaaagttctt agatatccgt
     1441 cagaagcatt tgcctgccag ccataggcaa cggagccctt ccctgaatca tcaatgcttg
     1501 agatcattac attgaaatcg tttgcccatc cgtccgctgc agtcggaccg gaggcaggct
     1561 tagggttcag ttccagtgaa ggagtgaaca tctgctcggg aagcattgca ctgttgtaaa
     1621 gcgcattcat cattttgtat ttgagtgtcg agccgctttt ctgatagaca acagaaagac
     1681 ctcttctcac tgtgctcaat cctcctccgg atctgtagtc aggctcctga ccttcaatca
     1741 gaaagactcc tttgtaattg ctgtaatcat ccacactcgc aggtgtatgt ttcagaataa
     1801 ggttgaatac aatgcttgaa gttgtattgt ccagttttgt aatttttttt gcaatctggg
     1861 tttttgtaaa gaataccggt cttgattctg tatccgtctt gtccctttcc atcgtagccg
     1921 ccttgaacac cagtccggaa ggagcagtaa tcgaattcat ttcctgtacg agatcatatt
     1981 tttctccctt ggcaggaaga ctgtttttgg acttctggga tacacatgcc atcgcagtga
     2041 aaattcc
//
LOCUS       DAOQGJ010000658         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37431_length_2044_cov_2.896430, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000658 DAOQGJ010000000
VERSION     DAOQGJ010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37431_length_2044_cov_2.896430"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(836..1420)
                     /locus_tag="PK683_10675"
     CDS             complement(836..1420)
                     /locus_tag="PK683_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08958.1"
                     /translation="MSSSKENYTEFESECLKLSALFGSTSIILADNMFTNFKSFTKEF
                     GAMESIFNGIVKKYPSNSLVQEYFTLDPEKIDMTNVMQIPDQIYSVFEGKGISNSELK
                     AYLEIPSIRKLKEEGAAAGLFKRLSYACMIVAVIAKKILEDKSHEERGEFFQAIYDCA
                     EEIAKTAKEGWFGLGDAVSKNEAAILAKLKENLL"
     gene            1572..>2044
                     /locus_tag="PK683_10680"
     CDS             1572..>2044
                     /locus_tag="PK683_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08959.1"
                     /translation="MIETRNHWISRAPIQIHQCRSGVIRLQGKKYNMIAANYFTDNED
                     IIRHFDKIINWKELIDNYENNFQDAEEYKKTGNDRLSFAPTNYEEALDYYRSSLDTAG
                     DLAGNELSQQVREIEKTGLKYDNGKVIFPEQMIKAVEKIKDAGILGLLKNPFALTP"
BASE COUNT          618 a          417 c          408 g          601 t
ORIGIN      
        1 gtctgagacc cgtctctaca ttcccacaga aaagcatccc cctcagcccc tctccgttgc
       61 ggcatcttca cccgccaatg gagaggggaa agcggagctg gggtgaggtc ggcccgaagg
      121 ctgtcaggta aatttatcct gaacacaaaa aattttctca gtgcttacca gaaattttct
      181 caggctcacg aaaacttttc tcaggctcac acagaccaaa actcaggctc acaaaaactt
      241 ttctcagtga gtttataaat gatgacaggc tctttttttt atttgacagg gctcaaaatt
      301 ttcaaacgaa aggagtgaaa tgaaaatttc aatgattttg ccggtgaaac ggcggaagga
      361 gcagttctct gcaggtttgt ttcgaacata atctaattca gtgaagagtt taaccacgaa
      421 gaacgcgaag ttcacgaagg catagaaccc aattcctttt attttttttc gtgttttttg
      481 tgcctttcgt ggtgaatcct ttttctttcc agaagacgga tctgagatcc gtctctacgg
      541 gctttaaatc agaaaaattc taaccttaga tcagaaaaat tctaacctta aatcagaaaa
      601 attctaacct tagattaaaa aaattctaac cttagatcag aaaaattcta accttagatc
      661 agaaaaattc taaccttaga ttttttttgt ctgacaggct gtcagaattt ttgacgaaag
      721 ggttcttttc ccaaaggaaa gtattctcat cagcccctct ccacaatgtc aagaacagcg
      781 cgagcggaga ggggtgccgg aggcggatga ggttacacac accgatgttt ttatatcaca
      841 gcaaattctc tttcagtttg gcgaggattg cagcctcatt tttgctgact gcgtctccca
      901 gtccgaacca gccttctttg gcagtttttg cgatttcttc cgcgcagtca tagatggcct
      961 gaaagaattc acctctttcc tcgtgacttt tgtcttccag gatttttttt gcaattacag
     1021 ccacgatcat acaggcataa ctcagtcttt tgaacagtcc tgccgcagca ccctcttcct
     1081 tgagttttct tatagaagga atctccagat atgctttcag ttccgaattg gaaattccct
     1141 ttccttcaaa aacggaataa atctgatccg ggatctgcat cacatttgtc atatcgattt
     1201 tttcaggatc taaggtaaaa tattcctgca caagtgaatt ggaaggatat ttctttacaa
     1261 tgccgttgaa gatagactcc attgcaccaa attcctttgt gaaggatttg aaattggtga
     1321 acatattatc tgcaagtatg atggatgtgg aaccgaacag agccgaaagc ttcaggcact
     1381 ctgattcaaa ctctgtataa ttttctttgg aactgctcat ttttgtttcc tttatctgta
     1441 tgtaaatgtt ttacgcaaga gtaatcaggg atgttcttat gtaaactttt tttaggcaaa
     1501 ttcgattaca tcgaatttga atctgctttt aaaaaaggaa aaaaatcatt tccgaaattg
     1561 gtaaaaatat tttgattgaa actcggaatc attggatcag cagagcaccc atacagattc
     1621 atcagtgcag atccggtgtt atcagactac agggaaaaaa atacaatatg atcgcagcaa
     1681 actattttac ggacaacgaa gacattattc ggcattttga taaaatcatc aactggaaag
     1741 agctgattga taattatgaa aataattttc aggatgcgga agaatacaaa aagacaggga
     1801 atgacagact ttcttttgca cctacaaatt atgaggaagc tctggactac tacagatcga
     1861 gcctggacac tgcaggggat ctcgcaggaa atgaactctc tcagcaggtc agagaaattg
     1921 aaaagaccgg tctgaagtac gacaacggga aagtgatttt tcctgagcag atgatcaagg
     1981 ctgtggagaa gatcaaagac gcaggcattc tgggactgtt gaaaaatcca tttgctctga
     2041 ctcc
//
LOCUS       DAOQGJ010000659         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37438_length_2044_cov_2.592760, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000659 DAOQGJ010000000
VERSION     DAOQGJ010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37438_length_2044_cov_2.592760"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PK683_10685"
     CDS             complement(<1..604)
                     /locus_tag="PK683_10685"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNH08960.1"
                     /translation="MRSNTLNLGIFNGDVWLKFKIRNISENSDWLIEFAHPHIRTFEF
                     YEFQEGELSKKGTTGILFPLNSKEIPNKGFLIPVNLNYGEEKEYYFHLQSGTSIIVNL
                     HLWEKNSYIVQASVENFLYGIFLGIMAVMAFYNLFIFLSTGESAYLYYILYIICFTIM
                     QEAKNGFMYVTFLQAYPVISNNIVPLFIPLAVVFSAFFAKK"
     gene            497..736
                     /locus_tag="PK683_10690"
     CDS             497..736
                     /locus_tag="PK683_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08961.1"
                     /translation="MSEFDEPVGIFRNVSDLEFQPYITVENSQVQCIRTDTLEKELIV
                     FFQDFIECETRFFQNLNLRGDVLFFFFAGCDGVRE"
     gene            1165..1878
                     /locus_tag="PK683_10695"
     CDS             1165..1878
                     /locus_tag="PK683_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08962.1"
                     /translation="MGNGIFLCVDDDITVLNALRVILADTFDGGHTVEIAESAEEALG
                     ICQELKDEGKELSVIISDFIMPNMNGDELLVRLHSVSPRTVKIMLTGQSDLQGVKRAI
                     NEANLYRFLEKPFNNSDMMLTAKSALQAYNRERELEKRNMELEMANTMLEQKVKERTF
                     ELNKSLNIIKEDLALAKKIQKNILRIKPSLLNDLHIEVNYSPMTEVGGDFYDITRLND
                     SIYRVFLADATGHGVQAAR"
     gene            complement(1854..>2044)
                     /locus_tag="PK683_10700"
     CDS             complement(1854..>2044)
                     /locus_tag="PK683_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08963.1"
                     /translation="PTVDEYLREMEHLRTISDLINPKLVATHAGLAATKQIADSGLRP
                     LITTVATLNQAYLAACTP"
BASE COUNT          652 a          376 c          448 g          568 t
ORIGIN      
        1 atttttttgc aaaaaaagcc gagaatacta ctgcaagcgg aataaacaga ggaacaatat
       61 tattcgaaat cacaggatag gcctgaagaa aagttacata cataaatccg tttttggcct
      121 cctgcataat tgtaaaacat attatatata aaatataata taaatatgca gattctccag
      181 tggaaagaaa aatgaacaga ttgtaaaatg ccatgactgc cataatgccc agaaatattc
      241 catagagaaa gttttcgact gaagcctgca ctatatagga attcttctcc catagatgaa
      301 gatttacgat tatggaagtt ccggactgaa gatgaaaata gtattctttt tcttctccat
      361 agttcagatt gaccggaatc aaaaatcctt tgtttggaat ctctttggaa ttcagaggaa
      421 ataaaatccc agtcgttccc tttttggaga gttctccttc ctgaaattcg tagaactcaa
      481 aagttctgat atgaggatga gcgaattcga tgagccagtc ggaattttcc gaaatgtttc
      541 tgatcttgaa tttcagccat acatcaccgt tgaaaattcc caggttcagt gtattcgaac
      601 ggatactttg gaaaaggagc tgattgtttt ttttcaggac ttcattgagt gcgagacccg
      661 gttttttcag aatctgaacc tgagaggtga tgttcttttc tttttcttcg ccggctgtga
      721 tggagtcagg gaataaatta actgagaaaa atgttaaaaa aaaaccgtac agtatctaca
      781 gagactgatt aaattacaca tttcttatac actccatcct gcctgcagtt taaatgagca
      841 cccggatttc atcggaataa agacctgcaa aaaaatgcag attttaaacc tgaattgaag
      901 tcaagggcct gattaatgcc gcaggttaaa ctgttatggg aagtcaaaaa attctgcaag
      961 aagatttttg cacagttgaa ttccgcgaaa cagtcatccg cagagacttt ttattttcag
     1021 ctgcagaata ttcagtcctg ctgaatgtat cgaaatctga gtaaatttat tgacgccttg
     1081 acggagaatt ggaagatttt aagaaatact gaatggcaga ctgaaaaaaa tcttatttca
     1141 gaataaatcg acggggcagt aagtatgggc aatggaattt ttttatgcgt ggacgatgat
     1201 attactgttc tgaatgcatt gagggtaatc ctcgcagaca cttttgacgg aggtcataca
     1261 gtagaaattg ctgaaagtgc tgaagaggcc cttggaatct gtcaggaact caaagatgag
     1321 ggaaaagagc tgagtgtgat tatatctgat tttataatgc cgaatatgaa cggggatgaa
     1381 cttcttgtcc gtctgcattc cgtcagtccc agaacagtca agattatgct gaccggtcag
     1441 agtgatttac agggagtcaa acgtgccatc aacgaggcga atctgtaccg gtttctggaa
     1501 aaacccttca acaattcaga tatgatgctt actgcaaaat cagcattgca ggcatacaac
     1561 cgggaaagag aactggaaaa gcgcaatatg gaactggaaa tggccaatac tatgctggaa
     1621 cagaaagtga aagaacggac attcgaactg aataaatctc tgaatattat aaaggaagat
     1681 cttgctcttg ccaaaaaaat tcaaaaaaat attctacgta taaagccttc tcttctgaat
     1741 gaccttcata tagaagtgaa ttacagtccc atgacagagg tcggcggtga tttttatgat
     1801 attaccaggc tgaatgattc tatataccgc gtttttctgg ccgatgccac tggtcacgga
     1861 gtgcaggccg cgagataagc ctgattaagc gtcgccactg tggtgatgag cggccgcaaa
     1921 ccggaatccg cgatctgttt agtggcggcg agtccggcat gggtcgcgac cagcttcggg
     1981 ttaatcaggt cggaaatagt gcgcaaatgt tccatctcgc gcagatattc atcgaccgtc
     2041 ggac
//
LOCUS       DAOQGJ010000660         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37444_length_2044_cov_2.148819, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000660 DAOQGJ010000000
VERSION     DAOQGJ010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37444_length_2044_cov_2.148819"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PK683_10705"
     CDS             complement(<1..551)
                     /locus_tag="PK683_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH08964.1"
                     /translation="MARDNKILILEDSRTFSGYLSILIRKKLGLESIVFSRFQDFVKR
                     ADEFSRGINICICDIHLPDVKADEHIDFLIERGISVIVVTAEFNFASREEILNKKKIV
                     DYVIKDRKEEMDYLLYLISRLHRNPGIKVVIADDSLLQRRIMKDFLSAHLFQVSEVKN
                     GIECLELIQKEKNVKLVITDYNMP"
     gene            complement(555..1532)
                     /gene="nadA"
                     /locus_tag="PK683_10710"
     CDS             complement(555..1532)
                     /gene="nadA"
                     /locus_tag="PK683_10710"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002152413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNH08965.1"
                     /translation="MDKDSLIKKLSATYLPQEIDSRLPLIEEISSLKKEKNAVLLGHN
                     YMTPDVFYGVSDIVGDSLYLSKAAGETNADIILFNGVHFMAETAKILSPQKKVLIADL
                     KAGCSLAESITRDDVKKLKLQYPGVPVVTYVNCSAEVKAETDICCTSANAVQIVNSID
                     SDTVIFLPDEYLASNVQKHTKKKLITHPGRCMVHEMYTKDDIIMARRQFEELTVVSHP
                     ECSSDVVDASDFAGSTSEMAGFIEKSGAKNIMLITECSMGDNLRSTFPEKHFVSTCQT
                     CPHMKKITLAKIRDALLYEQYEIILDEEIIRSGKKSVERMLEISYSRKN"
     gene            complement(1534..>2044)
                     /locus_tag="PK683_10715"
     CDS             complement(1534..>2044)
                     /locus_tag="PK683_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08966.1"
                     /translation="GILAMTKRTISLLFFSFFIIGCPVPQDNDLEKRIITALLNQKVN
                     PVDEYCSNFAITESLCAANPDSSSVTCSDSQLETVKKGIMPEEKRTDAVRNAYFTCWN
                     KCNLIFNTNEPICNSSSKFKTTKNYRDAQKSDSTAQSKNWILCMNNCNKGGSQEQGLT
                     ETYYPKPAY"
BASE COUNT          539 a          450 c          386 g          669 t
ORIGIN      
        1 ggcatattgt aatcagtgat caccagtttg acgttctttt ccttctgtat cagttcgagg
       61 cattcaattc cgtttttgac ctcagacacc tgaaacagat gcgcggaaag aaagtctttc
      121 attatccttc tctgaagaag tgaatcgtct gcaatgacaa ctttaattcc cggatttctg
      181 tggagcctgc tgatcagata cagaagataa tccatttcct cctttctgtc cttgatcaca
      241 tagtctacaa ttttcttctt attgagaatc tcttctcttg aagcaaaatt gaattctgca
      301 gtgactacaa tgaccgatat ccctctctca atcagaaaat caatatgttc atcagcctta
      361 acatccggaa gatgtatgtc gcagatgcat atattgattc ctctgctgaa ttcatcagcc
      421 ctttttacga agtcctgaaa ccttgaaaac acaatggatt ccagcccaag tttctttctg
      481 atcagtatag agagataacc ggaaaacgtt ctgctgtctt ccagaatcag aatcttattg
      541 tctcttgcca agtttcagtt ttttctgctg taggaaattt cgagcatccg ctctacggat
      601 ttttttccgc ttcttatgat ttcttcatcc agaatgattt catactgctc gtaaagaaga
      661 gcatctctga tttttgccaa agtgattttc ttcatatgcg gacaggtctg acaggtggat
      721 acaaaatgtt tttcaggaaa ggttgaacga agattgtctc ccatggaaca ctctgtaatc
      781 agcatgatgt tctttgctcc tgatttttca atgaatcctg ccatctccga tgtggatcct
      841 gcaaaatcgg atgcatcaac cacatcgctg ctgcactcag gatgagatac gactgtcagt
      901 tcctcgaact gtctgcgtgc catgataata tcatcctttg tatacatttc atgtaccatg
      961 catcttcctg gatgagtaat cagctttttc ttcgtatgtt tctgcacatt tgaagccaga
     1021 tattcatccg gaagaaatat cactgtgtct gaatcgattg aatttacaat ctggactgca
     1081 tttgcagaag tgcagcagat gtctgtctct gcttttacct ctgcagagca gttgacatag
     1141 gtcacgaccg gaacccccgg atactgaagt ttcagttttt tcacatcatc ccgcgtaatg
     1201 ctttctgcaa gagaacagcc tgccttgaga tcggcaatca gcaccttctt ttgaggagaa
     1261 agaatcttgg ctgtttcagc cataaaatgg acaccgttaa aaagtataat gtccgcattt
     1321 gtttctccag cagccttgct cagataaagt gaatccccca ctatatccga aacaccgtaa
     1381 aatacatccg gtgtcatata attatgaccg agaagaactg catttttttc cttcttcaga
     1441 ctgctgattt cttcaatcag aggaagacgg ctatcaattt cctggggtaa gtacgttgca
     1501 ctcagttttt ttatcagact gtccttgtcc atatcagtat gcaggtttcg ggtagtaagt
     1561 ttcagtaaga ccctgttcct gacttcctcc tttattgcag ttgttcatac atagaatcca
     1621 gtttttagac tgcgccgtgc tgtcactttt ctgcgcatca cggtaatttt tagttgtttt
     1681 gaacttcgaa ctggagttgc agatcggttc attcgtatta aagatcagat tgcatttatt
     1741 ccagcaggta aaataggcat ttctcactgc atctgtcctt ttttcttccg gcattattcc
     1801 ttttttgact gtttccagct gagaatcaga acaagtgacc gaggaagagt caggatttgc
     1861 agcgcataac gattctgtaa tcgcaaaatt actgcagtat tcatctactg gatttacttt
     1921 ctgattgagc agagcagtaa taattctttt ttccagatca ttatcttgcg gaacaggaca
     1981 gccgataata aaaaaggaaa agaaaagcag gctgattgtt cttttagtca tggccaagat
     2041 ccct
//
LOCUS       DAOQGJ010000661         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37446_length_2044_cov_2.110106, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000661 DAOQGJ010000000
VERSION     DAOQGJ010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37446_length_2044_cov_2.110106"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2043)
                     /locus_tag="PK683_10720"
     CDS             complement(<1..2043)
                     /locus_tag="PK683_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH08967.1"
                     /translation="MNGLVVFQFVFEEGTDDYFARTRLLEKVREAEIPEGVDPQLGPM
                     SSPVGEIYRYVLESSENHTAMELRTLQDWVVYPKMLSIPGIADVVTFGGLLKQFHVIT
                     DPEKLIQYKLKLSDVIQSIRDNNLNTKRNILRQGEMGFPIRSLGAIRTIKDIENIVLK
                     EVNGVPVFVRDVGAVETYPSLPTGVLGYTFQEYGDGLPDTDVDSSIQGLVAMRRWGDT
                     EEMGQRIRDKVAEINEKYLPKGVRMRTSYDRGDLVNYTLRTIGLTLIEGIAIVILVLI
                     FFMGSIPAALVVAATIPLSMLFAFILMKLTGVTANLLSLGAIDFGIIVDGSVVIVENI
                     MRKYRDSSEQDRVMGIIPFTLHSSAEVGKEIFFSISIIILAYLPIFTFQRIEGRLFSP
                     MAFTLSYAIGGSMLLALTFVPVMMSYLFSKKFEAGDLAHIEWHNPVVHWMTHKYEGYI
                     GTFIANSKRTVQGAFAVVIVITAIGVKLIGTEFLPELDEGGFTMRAFWPVGIHLQQVR
                     SYMPKFREVIANNKQVNVILSQLGRNDDGTDPLGPNRLEVYIGLRDYSKWEEKITKAD
                     LLLRMKNDLEAEFPGVRLSFSQPIMDNLSEAIMGTIADLAIFIAGDDLREMRKIAEQI
                     LEIVKDIPGASEFGIEQEGEQTQLSINIDREKSARYGINVSEIQQVVEAAIGASRIS"
BASE COUNT          534 a          521 c          425 g          564 t
ORIGIN      
        1 ggaaattctg gatgcaccga ttgcagcttc cacaacctgc tgaatctcgg atacattgat
       61 tccgtacctt gcagatttct ccctgtcgat attgatcgag agctgtgtct gttccccttc
      121 ctgttcaatt ccgaattcac ttgcacctgg aatgtccttt actatctcca gaatctgttc
      181 cgcaattttc cgcatctcac gaagatcatc tcctgcaatg aagattgcca agtcggcaat
      241 tgttcccatg atcgcttccg acaggttgtc cataatcggc tgagagaaac tgagacggac
      301 cccgggaaat tctgcttcca gatcattttt cattctcaga agaagatctg cctttgtaat
      361 cttttcttcc catttggaat agtccctcag accgatgtac acttccagtc tgttcggacc
      421 cagcggatct gttccgtcat catttcttcc cagctgagaa agaatcacat tgacctgttt
      481 attgttggca atgacctctc tgaacttagg catatagctt ctgacctgct ggaggtgaat
      541 tcccactggc cagaatgccc tcatcgtaaa tcccccctca tccagctctg gaaggaattc
      601 tgttccgatc agtttcacac cgattgcagt aatgacaatt accactgcaa aagcaccctg
      661 aacagtccgt ttggaatttg cgatgaaggt tcctatatac ccctcatact tatgagtcat
      721 ccaatgcacg acagggttgt gccattcaat atgagccaga tcacctgcct caaatttctt
      781 ggaaaaaaga taagacatca ttacaggaac aaatgtcagc gccagaagca ttgatccgcc
      841 gattgcatag gaaagggtga atgccatcgg tgaaaaaagt cttccttcaa ttctctgaaa
      901 agtgaaaatc ggaagatagg caagaatgat gatgctgatc gaaaagaaaa tctccttgcc
      961 gacctctgca gaggaatgaa gtgtaaacgg gatgattccc attaccctgt cctgctcgga
     1021 ggagtcgcga tattttctca ttatattttc tacaatgacc acggatccgt ccacaataat
     1081 tccaaagtcg atcgcaccca aggacagaag attcgcagtc actcctgtca gcttcatcag
     1141 gataaatgca aaaagcatcg agagaggaat ggtcgctgca actacgaggg ctgcaggtat
     1201 gcttcccatg aagaaaatca ggaccagaat aactatggcg atcccttcaa tcagagtcag
     1261 tccgatagta cgaagtgtat agttgaccag atcccctctg tcataggaag ttctcattcg
     1321 aactcccttc ggcaggtatt tctcgttgat ctcggccact ttgtcacgga tccgctgacc
     1381 catttcttct gtatcacccc atcgtctcat agcaacgaga ccctggatgc ttgagtctac
     1441 atcggtgtca ggaagaccgt ctccgtattc ctgaaatgtg taaccgagca cgcctgtggg
     1501 aagggaggga taagtttcca cagcgcctac atcccttaca aatacaggaa ccccgttcac
     1561 ttccttcaat acaatgtttt cgatgtcctt gattgtccga attgcaccga gacttcgaat
     1621 tggaaatccc atttctccct gacggagaat gttccttttc gtgttcaggt tattgtccct
     1681 gatagactga atcacatcgg agagtttgag tttatactga atcagttttt caggatctgt
     1741 aattacatgg aactgtttta agaggcctcc gaatgtcact acgtctgcaa tacccgggat
     1801 ggaaagcatc ttaggatata ccacccagtc ctgcagtgtt ctgagttcca ttgcagtgtg
     1861 gttctcagag gattccagta catacctgta aatttcaccg acaggagaac tcatcggccc
     1921 gagctgagga tcgactcctt ccggaatctc tgcctccctt actttctcga gaagcctggt
     1981 cctggcaaaa taatcgtctg tgccttcttc gaatacaaac tggaatacaa ccagtccgtt
     2041 gatt
//
LOCUS       DAOQGJ010000662         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37464_length_2043_cov_3.284205, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000662 DAOQGJ010000000
VERSION     DAOQGJ010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37464_length_2043_cov_3.284205"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PK683_10725"
     CDS             <1..728
                     /locus_tag="PK683_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH08968.1"
                     /translation="DPVSIKKGLTDVKEAIQINFYVLDHPAYGRFLIDTGIGDVFKKD
                     KSEWPFTWIVKKGMGTDSLKIKTATGEWMSRNPGKINGVFFTHLHLDHIMGTNDISPD
                     TPLYVGPKESTFKSVMNMMVQGNTDEFLKGKTIQELDFSEPNSEQGLSILDFFGDKSF
                     LIFHSPGHTPGSLAFLIHSKDGLQLLLGDTCHSRFGWENNVPPGDFSDDKEQNKKSLT
                     ALQNIAKKFSVIKIHPGHQNLKE"
     gene            complement(790..921)
                     /locus_tag="PK683_10730"
     CDS             complement(790..921)
                     /locus_tag="PK683_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08969.1"
                     /translation="MKTTVEIPDDLFVQVKTAAAVRGMTFRDVLIRSLEAELAISSV"
     gene            complement(955..1617)
                     /locus_tag="PK683_10735"
     CDS             complement(955..1617)
                     /locus_tag="PK683_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNH08970.1"
                     /translation="MKYLVLLFSLLFILPAEAKLTKEQKKELRKTTKWLSSFDLRYAK
                     PWTPPGDSKSINMDCSNTARYLYKKALNIDLPSGGSYDMYKYYKSHRQSAKSPKKGNE
                     INVEKLSSKLKSGDLLFWINTHSDIDPSHTPPISHVMIYLGRSRKTGEMKMVGANTFG
                     KGDFTEGGGPDVYTFDPNEFIGCVRTDRKNRKSPCIKGKESKFFAFARPVIQSESEAA
                     DP"
     gene            complement(1667..1927)
                     /locus_tag="PK683_10740"
     CDS             complement(1667..1927)
                     /locus_tag="PK683_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08971.1"
                     /translation="MTPTILSKTGFLSLLISITLIQNCTSQSELNDKVKKRKKSCAAL
                     AVFLKSEEARTGVTNFNNNLTILKCYNNATSKGDAEITAAPW"
     gene            complement(1955..2031)
                     /locus_tag="PK683_10745"
     tRNA            complement(1955..2031)
                     /locus_tag="PK683_10745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1995..1997),aa:Arg,seq:acg)
BASE COUNT          592 a          387 c          423 g          641 t
ORIGIN      
        1 aagacccagt ttccataaaa aagggtctga cggacgtgaa agaagcaatt caaattaatt
       61 tttatgtatt ggatcatcca gcttatggaa ggtttttgat tgatactggt attggtgacg
      121 tctttaaaaa ggataaatct gaatggcctt ttacatggat tgtaaaaaag gggatgggta
      181 cggattcact caagataaaa actgctacag gtgaatggat gtctagaaat ccaggaaaga
      241 taaacggagt attttttact catttgcatt tggatcatat tatggggaca aatgatataa
      301 gccccgacac cccgctctat gtgggaccaa aagaatccac gttcaaatct gtaatgaata
      361 tgatggtaca aggaaacact gatgaatttt taaaaggaaa aacaattcaa gaattagatt
      421 tttcagaacc caattctgaa cagggtctgt ccattctgga tttttttgga gacaaatcct
      481 ttctaatttt tcacagtcca ggtcatactc ccggaagcct ggcatttttg attcatagta
      541 aagatgggtt gcagctttta ctcggtgata cctgtcactc caggttcggc tgggaaaata
      601 atgtaccacc tggtgatttt tcggatgata aggaacaaaa taagaaaagt cttactgcac
      661 ttcagaacat tgcaaaaaag ttttcggtga taaaaatcca tcccggccat cagaacctaa
      721 aagaatagaa aaaataatta tattccatac aagccaagcg actaaagccg cttggttttc
      781 tctaattcgc tatactgatg aaatggcaag ttctgcttcc aagctacgaa tcagtacgtc
      841 gcggaatgtc atcccgcgaa ctgcagccgc agtttttacc tgaacaaaaa ggtcatcggg
      901 aatttccact gtagtcttca ttgacccata ttcccatatt catacaatga ttttttaagg
      961 atcagcagcc tcggattcac tttgtattac aggtctcgca aaagcaaaaa atttactctc
     1021 ttttcctttt atgcagggac ttttcctgtt ttttctgtct gttctgacac agccgataaa
     1081 ttcatttgga tcaaatgtgt atacgtcagg cccaccgccc tcagtaaaat cacctttccc
     1141 gaaagtattc gcacctacca ttttcatttc tccggtcttt ctgcttcttc ccagataaat
     1201 cattacatgg ctgatggggg gggtatggct gggatcaatg tcgctgtgag tatttatcca
     1261 gaaaagaagg tcacctgatt tcagtttgga agagagtttt tcaacattga tttcgttccc
     1321 tttctttgga gattttgcag attgacgatg actcttataa tatttataca tatcataaga
     1381 tcctccggaa ggcaagtcta tgttgagggc ctttttgtac agatatctgg cggtattgga
     1441 acagtccata ttgatggatt ttgaatcacc gggcggagtc catggctttg cataccgcag
     1501 atcaaaactg gacagccatt ttgttgtttt gcgaagctct ttcttctgtt cttttgtaag
     1561 ttttgcttca gcagggagga taaaaaggag tgaaaaaagg agaaccagat atttcattaa
     1621 aaatgagaat tctgacagtc ctcattcagt caagaagatt tgctttttac caaggagcag
     1681 cggtgatttc agcatcacct ttggaagtcg cattgttgta gcatttcaga atggtaaggt
     1741 tgttgttaaa atttgtaact ccggttcttg cttcttctga tttaagaaaa accgccaaag
     1801 ctgcacagct cttctttctt tttttgactt tatcattcag ttcggattga gaagtgcagt
     1861 tttgaatcag tgttatggag atcagcaaag acagaaagcc ggtttttgag aggattgttg
     1921 gagtcatttt attgtacctg tgttgcaaat aaactggcgc gcccagaagg atttgaacct
     1981 ccaacctttt gatccgtagt caaatcctct atccagttga gctatgggcg caggatttta
     2041 ctg
//
LOCUS       DAOQGJ010000663         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37473_length_2043_cov_2.418008, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000663 DAOQGJ010000000
VERSION     DAOQGJ010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37473_length_2043_cov_2.418008"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PK683_10750"
     CDS             <1..1207
                     /locus_tag="PK683_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH08972.1"
                     /translation="KASSILTSDMKEYIKFITDKRFLIPLAILTVIEIFLQTGVFRPY
                     LKKNSYASNINRITDHILSHKEDHNPDILILGTSVAYQGLSPRILQNKMDSVGLKVQS
                     AAVPGSELIVQELVSEKILRKFDKVKLLVYVAETTMPWGSQWELGLPTLAMVGEFDKK
                     DFIPKVGNYGYNVHYDDWVYLMFKSVAYRRDISDLLTDPGKRMKQISKLLKNPNKNFY
                     DFENDHFEKVSSYPAETLEECIKKTENFGAPPYPDNSNPDHKKAVLETCALAKYTRDL
                     INDPHPAKKEQAKKNIELYFQRFRKVYEHYRKRNIKFLIVFAPYSHLMKDLGGDDKVN
                     VWKQNLEGIIGKENLWIADMQDAFDGKDSNDFCYDTIHLNHQGMELFSEKLGNFLLEK
                     ENLKRILDK"
     gene            1204..1608
                     /locus_tag="PK683_10755"
     CDS             1204..1608
                     /locus_tag="PK683_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNH08973.1"
                     /translation="MKKTAEKTVQKKKSANKDFHAEGTLTFAEAQNTVHGWIQSYGVR
                     YYSELTNTAILMEEVGELARLMARTYGEQSFKKSDRESSISGEIGDILFVLAALCNQM
                     NISMEDAFKSTIEKNTKRDSTRHKKNRKLNDS"
     gene            1598..>2043
                     /locus_tag="PK683_10760"
     CDS             1598..>2043
                     /locus_tag="PK683_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08974.1"
                     /translation="MTVKLLLLFAVTSTCITASISTLVDLNYKLDLLLALQAFFPWIT
                     FLYYYRKSRRERFLELQQSTELKMELEYRAVSDYFKNNLFTLNQPEKIFSEFQDFILS
                     EFHPEFCDIFYWSDEQGQFILIGGKSRRDYEEKFMLFDPMMIWLTEL"
BASE COUNT          671 a          417 c          440 g          515 t
ORIGIN      
        1 caaggcttcc tctattttga cttctgacat gaaagaatat ataaaattta tcacagataa
       61 aagatttctc atcccgctcg ccattctcac tgtcattgaa attttccttc agacaggagt
      121 attccgtccc tacctgaaaa agaattcata cgcatccaat ataaacagga tcactgatca
      181 tattctttct cacaaggaag atcataatcc tgacatactg atcctcggaa caagtgttgc
      241 ctatcaggga ctttcccccc gaattctgca gaataaaatg gattcagtcg gtttgaaagt
      301 tcagtccgct gccgttcccg gttcagagct gatcgttcag gaacttgtga gtgaaaagat
      361 tcttcgaaaa ttcgacaagg tgaaactgct ggtctatgtg gctgaaacca caatgccctg
      421 gggaagtcag tgggaactgg ggcttccgac tcttgccatg gtaggcgaat tcgataaaaa
      481 agacttcatt cccaaggtgg gaaattacgg ctataatgtt cattacgatg actgggttta
      541 tctgatgttc aagtcagtcg cctacagaag ggatatcagc gatcttctca cagatcccgg
      601 aaaaagaatg aaacagattt ccaaacttct gaagaaccca aataagaatt tttacgattt
      661 cgaaaacgat cattttgaaa aggtcagttc ctaccctgcg gaaacactgg aggaatgcat
      721 taaaaagaca gaaaatttcg gagctccccc ctaccctgac aattccaatc ccgatcacaa
      781 aaaagcagtt ctggagacct gcgctctggc gaaatataca agggatctga ttaatgaccc
      841 gcacccggcg aaaaaagagc aggcaaaaaa gaacatagaa ctgtattttc agagattcag
      901 aaaagtctat gaacattaca ggaaaagaaa cataaaattt ttgattgtat ttgctcctta
      961 cagtcatctg atgaaggatc tgggtggaga tgacaaggtc aatgtctgga aacagaatct
     1021 ggaaggaatt atcggaaagg agaacctgtg gatagccgat atgcaggatg catttgacgg
     1081 gaaagacagc aatgacttct gttacgatac aatccatctg aatcatcagg gaatggagct
     1141 gttttcggaa aaactcggga actttctgct tgagaaagaa aatttgaaga gaatacttga
     1201 caaatgaaaa aaacagcaga aaaaacagtg cagaaaaaga aatctgcaaa taaggatttt
     1261 cacgcagaag gaacactcac gtttgcagag gctcagaaca ctgttcatgg atggattcag
     1321 tcttacggag tcagatacta ttccgaactt acaaataccg caatcctgat ggaggaagtg
     1381 ggtgaacttg ccagactgat ggcaaggaca tacggggagc agtccttcaa gaaatcggac
     1441 agagaaagca gcatttccgg agaaatcggg gacatccttt ttgtgctggc ggcactctgt
     1501 aatcagatga acatcagcat ggaagatgct ttcaaaagca cgattgaaaa gaacacgaaa
     1561 agagacagca caagacacaa aaagaacagg aaactaaatg acagttaaac ttctgcttct
     1621 gtttgcagtg acttccacct gcatcactgc gtccatcagc actcttgtgg atctgaatta
     1681 taagctggat ctgcttcttg cactccaggc attcttcccc tggattacat ttctctatta
     1741 ttacaggaaa tccagaagag aacgttttct ggaacttcag cagtccactg aactgaaaat
     1801 ggaactggaa tacagggcag tttctgatta tttcaagaac aatcttttca ctctgaatca
     1861 gccggaaaag atattcagtg agttccagga ttttattctt tctgagtttc atcccgaatt
     1921 ctgcgatata ttctactgga gcgacgaaca gggacagttt attctgatcg gaggaaagag
     1981 cagaagagac tatgaagaaa aattcatgct cttcgatcct atgatgatct ggctcactga
     2041 act
//
LOCUS       DAOQGJ010000664         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37516_length_2041_cov_3.508056, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000664 DAOQGJ010000000
VERSION     DAOQGJ010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37516_length_2041_cov_3.508056"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..2029
                     /locus_tag="PK683_10765"
     CDS             356..2029
                     /locus_tag="PK683_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08975.1"
                     /translation="MTRFIIIFSAVLLTVFIGCSGTKVTKFEKPKTIFFIKKGAKLPA
                     YKDIKNPKKDLDISESDNPEVTAEVLVKKTNAVYYELKCPEKSAAVCGKDSVYVSGTS
                     GFYRLEQLEEMISKGSGIREGFMLVNPEEEEYFISTRDWFANPGRVTEVKLSKNSMLT
                     AIAMIESTDYKTQILGELLIVEKLAKENPESEDKVRTDTIIKKYAVLSSKKSGSSARI
                     TGLEGEVLEKIRSTYEKLEKSAVSGFPMRAQSYKSLAEQFNKLSKTPYLSEKIFAEIL
                     RDAVLAPQGGNGYITSASDVSAVLKKAKDLGENTEGSEFLVNWKVSIEPLKGVKISAD
                     KLDSSGASLGTEETALESVSAFADGDKFALKLKIAGGEFSVITKENGPYLLEGGPGLK
                     EYIKQINGRYGELLWNNEFGPGVVKSVLKDIPKGGYNPDTGMMEYSLDTGENVHYWIM
                     LEIIRRSPKFGAQKKDYAGKLDDPNAEFISWRQPKGEFLLKKYMYPRSESKETEEITC
                     MKDKGSEKLKISFLPSELKSENPRLIFAFESGNSVCDSFISSALNNESR"
BASE COUNT          662 a          360 c          467 g          552 t
ORIGIN      
        1 agaagttttt tcgctctgac tgaaaaggct gtactatttc agtacagcct tttcagtgat
       61 ttgaatcatc agatcatttt gcgattctaa aaaagagaat catcggtgct gcatttttta
      121 cctcgaactc gatactcctg ccaactgaaa aattcatttt ttaattctca gattgccaat
      181 cttgattgaa tttgagttct cgctgaaaca aacagaaaag atccggattc tgaaatatat
      241 ttattcatat agtaaaaacc ttcttttcat tcttcgactt ttcctgtgaa ctggtcaata
      301 gactgctgtg aaatttagag ttcacagaaa ccataagcaa gtggagcatt tgactatgac
      361 cagatttatt ataatatttt cggccgtatt gctgaccgtt ttcatcggct gttccggaac
      421 aaaagtgacc aagtttgaaa aaccaaagac aatatttttc ataaagaaag gagccaagct
      481 tccggcatat aaggatataa aaaatcctaa gaaggatctg gacatttcag agtcggacaa
      541 tccggaagtg acagcagagg ttctggtcaa aaaaacaaat gcagtctatt atgaactgaa
      601 atgtccggag aaatctgcag ctgtatgcgg caaagattca gtctatgttt caggaacatc
      661 cggattctac agactggaac agctggaaga aatgatttca aaaggttccg gaatcaggga
      721 aggctttatg ctcgtcaatc ctgaggaaga ggaatacttt atatctaccc gcgactggtt
      781 tgcaaatcct ggccgggtca cagaagtcaa gctttccaaa aattccatgc tcactgcaat
      841 tgcgatgata gagagcacgg attataagac tcaaatactt ggtgaactcc ttattgtaga
      901 aaaacttgca aaggaaaatc ctgagtcaga ggataaagtc cgtacagata ccattataaa
      961 aaaatatgca gtattgtctt caaagaaatc tggatcttct gcaaggatca caggtctgga
     1021 aggggaggtg ctggaaaaaa tcagatcaac ttatgagaag ctggaaaaat ccgcagtttc
     1081 agggtttcct atgagggcac agagctataa aagtctggct gaacagttca ataaactgtc
     1141 gaagacgcct tacctttctg agaaaatttt tgcagaaatt cttagagatg cagttctggc
     1201 accgcagggg ggaaacggat acattacttc cgcttccgat gtttctgctg ttctgaaaaa
     1261 agcaaaggat ctgggagaga atacggaagg atctgagttt cttgtgaact ggaaggtcag
     1321 catagagcct ctgaagggag tgaagatttc tgccgacaag ctggattctt ctggtgcttc
     1381 tttggggaca gaagaaacag ccctcgaatc agtctctgca tttgcagacg gagataagtt
     1441 tgctttgaag ctgaagattg caggcgggga attttctgta atcacgaaag agaacggtcc
     1501 ttaccttctg gagggaggtc cgggtctgaa agagtatatt aagcagataa acggaagata
     1561 cggtgaactg ctttggaaca atgaatttgg acctggtgta gtgaaatcag tattgaagga
     1621 tattccaaag gggggctaca atccggatac aggaatgatg gagtattctc tggatacagg
     1681 agaaaacgtt cattactgga taatgcttga aattatcaga agaagtccta agttcggtgc
     1741 acagaaaaaa gattatgcag gaaaactgga tgatccgaat gcagaattca tcagctggag
     1801 acagcccaag ggagaattcc tgctgaaaaa atatatgtat ccaagatcag agtcgaaaga
     1861 aacggaagaa attacctgca tgaaagacaa gggatcagag aaactgaaga tcagttttct
     1921 tccatctgag ctgaaatcgg aaaatccgcg gttaatattt gcttttgaat ccgggaattc
     1981 tgtctgtgat tcattcatca gttctgcatt aaataatgaa tcaaggtagt cattgtattt
     2041 t
//
LOCUS       DAOQGJ010000665         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37545_length_2040_cov_2.905793, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000665 DAOQGJ010000000
VERSION     DAOQGJ010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37545_length_2040_cov_2.905793"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PK683_10770"
     CDS             <1..1168
                     /locus_tag="PK683_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNH08976.1"
                     /translation="NLNIFLIIFLSACISEQKPPSAVSNPKPPPVVKKDPVKEKPVEK
                     KEPTVDEDLAFLMLKTEVPDSGKSSFSEVRQFLLPEKKKVYQLIFSEDSSHLVLVTNF
                     YIKKISLGSLEEVGTLYPRKLVDGGFTGVAFSPDLNRILAGSSWENTASVYDWAGKKE
                     KSFSCRKESFFAEDLVFLGSSKYALVCHNFASGKKDLYLYDSENSKTESTSLMYLKKQ
                     SDSSLLNYDSKTVRSVSADMKTNFRLKTDSEIWSLHYNPEKNLVFAGNNFGAEVWKIS
                     SKPKRIIKLDFKESDYPKSDIKLDHEFKNIVSFANDGSSVLIGNEKSAFIKDWKGNLR
                     QVLGGHKDYVTAVSLSGNGKYAATASRDRLIIWALNSSDGKKKVAQLSERKTRL"
     gene            complement(1329..1634)
                     /locus_tag="PK683_10775"
     CDS             complement(1329..1634)
                     /locus_tag="PK683_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08977.1"
                     /translation="MTDEGKILNLQSDVDYYVIGKFLPVERELSWLGYLTYSSTIIPY
                     VLTLGMIPSVEHNKITPSYYIFDKNFNQVASYHYRSEYWLVSTIWELDPKYPAEPDA"
     gene            complement(1782..>2040)
                     /locus_tag="PK683_10780"
     CDS             complement(1782..>2040)
                     /locus_tag="PK683_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08978.1"
                     /translation="KDNRKKMVFLGFLTFVLPGAIVLPPSFAKDVCYFKKTKYINLKC
                     LPDDSVCKSTVGIWSQEKKYANFFQISKNHNVCAISGFATI"
BASE COUNT          647 a          382 c          411 g          600 t
ORIGIN      
        1 aaatttgaat atatttttga ttatttttct ttctgcctgt atttcagagc agaagccgcc
       61 ttcagctgtt tcgaatccta agccccctcc tgtcgtcaaa aaagatccgg taaaggaaaa
      121 gcctgtcgaa aaaaaagaac cgactgtgga tgaagatctg gcatttctga tgctgaagac
      181 ggaagttcct gattccggaa aatcatcctt cagcgaggtc agacagtttc tgcttcctga
      241 aaaaaagaaa gtatatcagc ttattttcag cgaagacagc tcgcatctgg tgcttgtgac
      301 aaatttttac attaagaaaa tatctctcgg atcacttgaa gaagtgggta cactgtatcc
      361 cagaaagctt gtggatggag gatttacagg cgtagcattt tctcctgatc tgaacaggat
      421 acttgcaggg tcttcctggg aaaacactgc aagtgtatat gactgggccg gaaaaaagga
      481 aaaaagcttc agctgcagaa aagagagttt ttttgcagag gatctggtat ttctgggaag
      541 ttctaaatat gcgctggtct gccacaattt tgcatccggg aaaaaagatc tgtatcttta
      601 tgattcagag aactctaaaa cggaatctac cagtctgatg tatctgaaaa aacagtcaga
      661 cagctcgctt ctgaactatg actcaaaaac ggtcagatca gtcagtgccg atatgaagac
      721 aaatttccga ctgaaaacag attcggaaat ctggagtctt cattacaatc ctgaaaaaaa
      781 tcttgttttt gcgggaaaca atttcggtgc agaggtctgg aagatatcct caaagcccaa
      841 acgaattatc aagctggatt ttaaggagtc tgattatcct aaatctgata ttaagctgga
      901 tcatgaattt aagaatattg tatcctttgc aaatgacgga agttctgttc tgatcggaaa
      961 tgaaaagtct gcattcatca aggactggaa aggaaatctg agacaggtgc tcggaggtca
     1021 caaggattat gtgactgcag tatcgctgtc tggaaacgga aaatatgcag ccaccgcaag
     1081 ccgcgacaga ctcattatct gggcactgaa ttcctctgac ggaaaaaaga aagttgctca
     1141 gttgagcgaa agaaaaacaa gactttaaga tcagttctct gttcatctga atgcatatcc
     1201 gcagaagggc ttttatgccg ctctgcagat tcatttcagc atttgttttt ctcgcagaac
     1261 ttggccaatt cttttttgaa ttctatcaca gatggagtga aattaaaaag atcatttgcc
     1321 ggtacctgct atgcatccgg ttcggcagga tattttggat caagttccca aatcgtactg
     1381 acaagccagt attcactgcg atagtgataa gaagcaacct gattgaaatt tttatcaaat
     1441 atataatagg aaggagtgat tttattatgc tctacagaag gaatcattcc taaagtaaga
     1501 acataaggaa taatggtgga agaataggtc agatatccca gccagctcaa ttctctttcc
     1561 acaggcagaa attttccaat tacataataa tctacatccg attgcagatt caggattttt
     1621 ccttcatcag tcaattgaaa tgtttcggca aaaccgtata cagactgatt tccatattca
     1681 ttaaaatata aatcagcaaa ttttctgaac tttttatctg ctggatcaat agacgttgga
     1741 tctgctttcc cttttcgaaa ttccctaaaa aaactgtcag ctcaaatggt ggcaaaccca
     1801 gatattgcac agacattgtg atttttagaa atctgaaaaa aatttgcata tttcttttct
     1861 tgcgaccata ttcctactgt tgatttgcat acactgtcat ctggcagaca ttttaagttt
     1921 atatattttg ttttcttaaa gtaacagaca tctttagcaa aggaaggcgg aagaacgatt
     1981 gcacctggaa ggacaaaggt tagaaatcct aaaaatacca tttttttccg attgtccttt
//
LOCUS       DAOQGJ010000666         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37549_length_2040_cov_2.684635, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000666 DAOQGJ010000000
VERSION     DAOQGJ010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37549_length_2040_cov_2.684635"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PK683_10785"
     CDS             <1..1200
                     /locus_tag="PK683_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH08979.1"
                     /translation="IFYLFLFSILPVFSESIYDCIDSKNLNCLIGVLEKNPRSKDQIS
                     PDGYSPLQKTVLSEWVPGMKQILSVKPNLNYQSKTEKGTAVLFAALKGKNEIISPILE
                     AGADPNIISNHDFLPFASAVEKNCSNDTLELFEKYKANLNPKKGETPLGAAIKKKDWK
                     CTEKLLDKGADIKLVPAAFHHAIADRNKDMVKLFLQKGANPNLPGRINNAFFMALMSK
                     NQGIIDELMDSAKPDLKVQDEGETLLHSALKTGNEKFADYFVENSPLPTGKTKVTKET
                     LLHFAAKGGNLKYCKFFLEKGLNVNEGDFEGSTPFLILASSGPKDLNTEVIEEFLKYK
                     PNLDIAYKDTKQTAMHYFMNKKNKKAVKLLLQNGANPDLRDNNGQTVRAFYTKERDSD
                     ILELMKR"
     gene            1209..1595
                     /locus_tag="PK683_10790"
     CDS             1209..1595
                     /locus_tag="PK683_10790"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HNH08980.1"
                     /translation="MKRELLESIVLKSAGILLFVLGLDKLQPFLPHPEAAEPAAVFKK
                     AMFDTWYFLKLVGLTEISVGTMFISGYFIRLGLVILAPLSINFLFYHLFLDLMNIPAA
                     VFILLFNIYFAWKYKDSYKEVLRMQK"
     gene            1592..>2040
                     /locus_tag="PK683_10795"
     CDS             1592..>2040
                     /locus_tag="PK683_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH08981.1"
                     /translation="MNHSEYVLSVNMAMTIDGKTARPDGKWYGLSSRNDKIRMDKYRS
                     EADALLLGKNSLLNDDPAIKIRYQENADDPVPVILIRNGTIPASLRVFQSSQKPIIIC
                     ADNNYSNLKNLASLAEVVSLGTEIRPAEVIELLIRRKLNKILLEGGPT"
BASE COUNT          658 a          399 c          420 g          563 t
ORIGIN      
        1 attttctacc ttttcttatt ttccatcctt cctgtttttt cggaaagcat ttatgactgc
       61 attgacagca aaaacctgaa ctgcctgatc ggagttctgg aaaaaaatcc cagatcaaaa
      121 gatcagatca gtcctgacgg atattctcct ctgcaaaaga ctgtactgtc tgaatgggtt
      181 cctggaatga aacagatttt aagtgtcaaa cctaacctga actatcagtc caaaactgaa
      241 aaaggaaccg ccgtactttt tgctgcctta aaggggaaaa atgaaatcat atccccaata
      301 cttgaagcag gagccgatcc aaatatcatt tccaatcatg attttcttcc ctttgcatct
      361 gcagtggaga aaaactgttc caatgacact ttggagcttt tcgaaaaata caaggctaat
      421 ctgaatccca aaaagggaga aactccacta ggagctgcaa taaagaaaaa ggactggaaa
      481 tgtactgaaa aactgcttga taaaggcgct gacattaagc ttgttccggc ggcatttcat
      541 catgcaattg cagacagaaa caaggatatg gtgaaacttt ttctgcagaa aggtgcaaat
      601 ccgaatcttc cgggaagaat taataatgct tttttcatgg cactgatgag caagaatcag
      661 ggaatcatag atgagcttat ggattccgct aaacctgatt taaaagttca ggatgaaggg
      721 gaaactcttc tgcattccgc gttgaagaca ggaaatgaaa aatttgctga ttattttgta
      781 gaaaattccc ctctgccgac aggcaagact aaagttacca aagaaacact tctccatttt
      841 gcagccaaag gcggaaatct gaaatactgc aaattttttc ttgaaaaagg gttaaatgta
      901 aatgaaggcg attttgaagg gagcactccg ttcctcattc tggcttcatc cggacctaag
      961 gatctgaata cagaggtaat agaagagttt ctgaaatata aaccgaactt ggatatcgct
     1021 tacaaagata caaaacagac tgcaatgcat tattttatga acaaaaaaaa taaaaaagca
     1081 gtaaaactcc ttctgcagaa tggagcaaat ccggatctca gggataataa cggacagaca
     1141 gtcagagctt tttatactaa agagcgtgac agtgatattc tggaactgat gaaaaggtaa
     1201 aggaacatat gaaaagagaa ttacttgaat cgattgtttt aaaatctgca ggaattctcc
     1261 tttttgtact tgggctggat aaacttcagc cctttctgcc tcatcctgaa gcggctgaac
     1321 cggctgctgt ttttaaaaag gcaatgtttg atacctggta ttttctgaaa ctggtgggat
     1381 tgaccgagat ctcagtgggc acaatgttta tcagcggata tttcatcaga ctggggctgg
     1441 taattctggc tcccctcagc atcaatttcc ttttctatca cctttttctg gatctgatga
     1501 atattcctgc tgcagttttc atacttctct ttaatatata ttttgcatgg aaatacaagg
     1561 attcttacaa agaagtttta agaatgcaga aatgaaccac agcgaatatg tcctatcagt
     1621 caatatggca atgacaattg acgggaaaac agccagaccg gacggaaaat ggtatggcct
     1681 gtcttccagg aatgataaaa tcaggatgga caagtacagg tctgaagctg atgcactcct
     1741 gcttgggaaa aattctctgc tgaatgatga tcctgcaatt aaaatcaggt accaggaaaa
     1801 tgcagatgac ccggttccgg tgatcctgat ccgaaatgga acgattcctg ccagtcttcg
     1861 agttttccaa tcctctcaaa agcctattat catatgtgca gataataact attccaattt
     1921 aaagaatctg gcctctctgg ctgaagttgt ctcacttggg acagaaatca ggcctgcgga
     1981 ggtcattgag ctccttatcc ggagaaaact gaataaaatt ctgctggaag gaggtcctac
//
LOCUS       DAOQGJ010000667         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37550_length_2040_cov_2.664987, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000667 DAOQGJ010000000
VERSION     DAOQGJ010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37550_length_2040_cov_2.664987"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..496
                     /locus_tag="PK683_10800"
     CDS             68..496
                     /locus_tag="PK683_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08982.1"
                     /translation="MNIDWELAERIQCTDAEREECVPLMKTLVSFAQKSRAGGLLVLD
                     DEVDNIRTSVEGTASFFKTGIELVIDGTDPEIIAEILSFKIMAGNYKGLPLLMRCIIL
                     AGVMGIQAGDNPRILADKLLSFFPDIYLEKKKPTIYFPED"
     gene            complement(738..1883)
                     /locus_tag="PK683_10805"
     CDS             complement(738..1883)
                     /locus_tag="PK683_10805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH08983.1"
                     /translation="MRKKIVLFFILFLAVLTGLSFFSIYTAQNNEKTWKTANRSFDVM
                     RKLKETDSHLNDLENYKKLYQETGNSEYLYLFEQKLHFFDSFLINQELERDFEDIPEQ
                     RTLYDSFLKHFHSIMKCQNPDCSNEKGEDVRHARTLIQKNSDELNRMEVIIIDLEQNE
                     KKNLETNREQHERDLSTITYITFFGSIFSFITSLILFYYVKRDLLLKSLREEELERIS
                     YTDELSQLYNRRGFIQKGKVLFEDAKSRKINLYLFYIDMDGLKSINDNFGHSSGDEAI
                     KCVAEILRHSFRLSDVIARLGGDEFAVLVAENNVLPEGISKRLSKKMHEFNERKEKAF
                     KIDFSVGFKSLSNDYMSLEAMLMEADSEMYRHKKDKKVKQEENTEKK"
BASE COUNT          586 a          396 c          401 g          657 t
ORIGIN      
        1 attttactga gtcgtgaaca aataaatacg caaagacttt tgaaaacgag tataaagggg
       61 tattgcaatg aatatagatt gggaattagc cgaaagaatt caatgtactg atgcggaaag
      121 agaggagtgc gtgcctttga tgaaaacact ggtcagtttt gctcaaaagt ccagagcggg
      181 aggactgctt gtgctcgatg acgaagtaga caatatcagg acaagcgtgg aaggaactgc
      241 ttcttttttt aaaacaggta ttgagcttgt cattgacggt acagatccgg agataatagc
      301 tgaaattctg tcgtttaaga ttatggctgg gaattacaag ggccttcctc ttctgatgag
      361 gtgcattatc cttgcaggag taatgggcat acaggcaggc gataatccta gaattctcgc
      421 cgacaaactg ctttcctttt ttcctgacat ttacttggaa aaaaagaagc cgacaattta
      481 ttttccagag gactgattca attttcctgc ttttcagtga taagtatttt tattttcagt
      541 cattctgccg gcgcaggatg tccgtcagga cttaagactt ttctgagatt tcgatcagct
      601 catagagctg aacgaaagaa gcttcgcagc tttcatattt gggagatcag aaatcaggat
      661 aataaaagtc tgaaatgcag gaatttattg tttaaaaaaa acgaagagtc tgaatcatcc
      721 gggttcagac aaacagttca tttcttttcg gtgttttctt cctgttttac tttcttatct
      781 ttcttatgcc tgtacatttc agagtcagct tccatcagca tggcctccag actcatataa
      841 tcgttggaaa gggatttgaa tcctacactg aaatcaattt taaatgcttt ttcttttctt
      901 tcattgaact catgcatttt tttggaaagc cttttgctga ttccctcagg aagcacattg
      961 ttttcagcga ctaggactgc gaattcgtct ccacccagtc ttgcaattac atctgaaaga
     1021 cggaatgaat gtctcagtat ttccgcaacg catttgatcg cctcgtcacc cgacgaatga
     1081 ccaaaattgt cattgatgga ctttaacccg tccatatcta tataaaataa atataaatta
     1141 atttttctgg acttggcatc ttcaaacaga acttttcctt tctgtatgaa tcctcttctg
     1201 ttgtaaagct gggaaagttc atctgtatag gaaatccttt ccagttcttc ttctcgaaga
     1261 ctcttcagaa gcagatccct tttcacataa tagaaaagta tgagtgaggt aataaatgaa
     1321 aaaattgaac cgaaaaaagt gatgtaagtg attgtgctga gatcacgttc atgctgttcc
     1381 ctgttcgttt ccaggttctt tttttcattc tgttcaagat caatgatgat gacttccatc
     1441 ctgttcagct catctgaatt tttctgaatc agagttcttg catgtcgtac atcctctccc
     1501 ttttcattgg aacagtcagg attctgacat ttcataatgg aatggaaatg cttcaggaaa
     1561 gaatcgtaca gggttctctg ctcaggaata tcttcaaaat ctctttccag ttcctgattg
     1621 atcagaaagg aatcaaagaa atgaagtttc tgctcaaaca gatacagata ctcgctgttg
     1681 cccgtctcct ggtaaagctt tttataattt tccagatcat taaggtgaga atctgtctct
     1741 ttcagcttcc tcattacatc aaaggatctg tttgcagttt tccaggtttt ttcgttgttc
     1801 tgtgctgtat aaatagagaa aaaggaaagc cctgtcagaa ctgcaagaaa gagaatgaag
     1861 aagaggacaa ttttttttct cataaagtct ccgtgcaggc attcacctgc acgggatatg
     1921 aatcaggaga tccgctctgc tactaattca gcgatatctt ttacttttac agaatccgtt
     1981 ttttccgcat tcttgacacc gtcagtaatc atagtcatac agaaaggaca tccgactgca
//
LOCUS       DAOQGJ010000668         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37648_length_2037_cov_2.571140, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000668 DAOQGJ010000000
VERSION     DAOQGJ010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37648_length_2037_cov_2.571140"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2037
                     /locus_tag="PK683_10810"
     CDS             <1..>2037
                     /locus_tag="PK683_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNH08984.1"
                     /translation="VDRLIGGEGKIDNLNAQEEKIQVRSNFSETAFFYPHLRTNEEGE
                     LVIAFKIPESLTKWKMLGFAHTEDLKFGMTENTLITQKDLMVVPNAPRFFREGDTINF
                     GVKINNLSEKDLTGNAELRLFDAVTMKPIDSLFRNTGPNKPGSGNSTIPAGAFSSKKG
                     QSDSIEWTLKIPEGIGAIAYRITAKAGKFSDGEEMTVPVLTNRMLVTESMPLPIRGKT
                     TKTFTMDKLLNSPAPGNYGKNDTLRHHRVTLEFTSNPAWYAVQALPYLMEYPYECIEQ
                     TFSRYYANSLSSHIVNSTPKIREVFEAWKSVPDSEKGALASNLEKNQELKSLLLEETP
                     WVRDAVGESERKKRIGLLFDLNKMASELGRAYKKIKEQQLSDGPWSWFKGMGPDRYIT
                     QHIVTGLGHLDHLGVKKLSETSDGQDMIKRAVGYLDREIKKDYDELKRLAGLKKINLD
                     DNHLGYLQIHYLYSRSYFLNLEIPKDTKPALDYYMGQAKKYWHNNRRYMQGMIALGLH
                     RFKDKTAPEAILASLRENSIESEEMGIYWKEGWGYFWYEMPIESQSLLIEAFSEIAND
                     EKFVDGMRTWLLKQKQTNDWKTTKATSEAIYALLLKGEDWLTTENQAEITMGSMKIDD
                     AALKSVKTEAGTGYFKLSYGPDKILPEMGRVTVTRKKSGVSWGALYWQYFENLD"
BASE COUNT          693 a          405 c          474 g          465 t
ORIGIN      
        1 acgttgacag actgataggc ggagaaggca aaattgataa tctgaatgcc caagaagaaa
       61 aaatacaggt cagaagcaat ttcagtgaga ctgcattttt ctatccgcat ctccgtacaa
      121 atgaagaagg agaacttgtc attgcattca agattcctga aagtctcaca aaatggaaaa
      181 tgctgggatt tgcacacact gaagatctga agttcggtat gacggaaaat actctgatta
      241 cacagaagga tctgatggtt gtcccgaatg ccccaagatt cttcagagaa ggagacacga
      301 tcaacttcgg tgtgaaaata aacaaccttt ctgaaaaaga tctcacagga aatgcagagc
      361 tccgtctgtt cgatgcagtc acaatgaaac cgatagacag tcttttcaga aatacaggac
      421 caaacaagcc tggatcagga aacagcacaa ttcctgcagg agcattcagt tccaaaaaag
      481 gacagagtga ttcaatagaa tggactctga aaatccctga agggataggc gcaattgctt
      541 accgaattac tgcaaaagca ggaaagttct ccgatggaga agagatgact gttcctgtcc
      601 ttacaaacag aatgcttgtc actgaaagta tgcctctccc tattcgcgga aagacaacta
      661 agacatttac aatggacaaa cttctgaact cccctgctcc gggaaattac ggaaaaaacg
      721 atactctcag acatcacaga gtcactcttg aatttacttc caatccggcc tggtatgcag
      781 tacaggctct tccttatctt atggaatatc cttacgaatg cattgagcag acattcagca
      841 gatactatgc gaacagtctg tcatcgcaca tagtaaattc gactccaaag atcagggaag
      901 tatttgaagc gtggaagtcc gtcccggaca gtgagaaagg agcacttgca tccaacctgg
      961 aaaaaaatca ggaactcaaa tcacttcttc tcgaagaaac accctgggtc agagatgcag
     1021 tcggcgaaag cgagagaaag aaacggattg gacttctgtt cgatctgaac aagatggcaa
     1081 gtgaacttgg cagagcatat aaaaagatca aggaacagca gctgtctgac ggaccatgga
     1141 gctggttcaa gggaatggga cctgacagat acataacaca gcatatagtc actggacttg
     1201 gacatctcga tcatctgggt gtaaaaaaac tcagcgaaac aagtgatgga caggatatga
     1261 tcaaaagagc tgtcggatat ctggacaggg aaatcaaaaa ggattatgat gaactgaaaa
     1321 gacttgccgg tctgaaaaaa attaatctgg acgacaatca tctcggttat ctgcagatcc
     1381 attatctgta ttccagaagt tatttcctga atctggaaat acccaaggat accaaaccgg
     1441 cacttgacta ttatatgggt caggcaaaaa aatactggca taacaacagg agatatatgc
     1501 aggggatgat agctctcgga cttcacagat tcaaggacaa gactgctcct gaagcgattc
     1561 ttgcttctct cagagaaaat tccatcgagt cggaagaaat gggcatctac tggaaggaag
     1621 gatggggata tttctggtat gaaatgccga tcgaatctca gagtcttctc attgaagcat
     1681 tcagtgaaat tgcaaatgat gagaaatttg tagatggaat gagaacctgg cttctgaaac
     1741 agaaacagac aaacgactgg aagacaacca aggctacaag tgaggcgatt tatgctcttc
     1801 tcctgaaagg tgaagactgg ctgacaacgg agaatcaggc tgagattaca atgggttcta
     1861 tgaagattga tgatgcagca ctcaagtcag taaagacaga ggccggaaca ggttacttta
     1921 agctttctta tggaccggac aaaattcttc ctgagatggg cagagttact gtgacaagaa
     1981 agaaatccgg agtcagctgg ggagctctat actggcagta ttttgaaaat ctggaca
//
LOCUS       DAOQGJ010000669         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37651_length_2037_cov_2.228557, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000669 DAOQGJ010000000
VERSION     DAOQGJ010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37651_length_2037_cov_2.228557"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..433)
                     /locus_tag="PK683_10815"
     CDS             complement(95..433)
                     /locus_tag="PK683_10815"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNH08985.1"
                     /translation="MARFYTNENFRLSIVLELRKLGNDVLTSLEAGNANQSIPDEKVL
                     EFSISENRILITFNRRDFIQLHNSKSEHSGIIVCTDDPDAARIAKKIHDAVSDKGLEN
                     QLVRIHRDSI"
     gene            complement(433..759)
                     /locus_tag="PK683_10820"
     CDS             complement(433..759)
                     /locus_tag="PK683_10820"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNH08986.1"
                     /translation="MTFIEMEKDIAEMSSREKALLFKLLADDLSKSFPGIEKKDGVSG
                     GSACIVRTRIPVWTLELARRNGLSEAQLLFDWPSLRAADLVNAWSYSVLHKEEIEKDI
                     EENLRA"
     gene            916..1365
                     /gene="rplM"
                     /locus_tag="PK683_10825"
     CDS             916..1365
                     /gene="rplM"
                     /locus_tag="PK683_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNH08987.1"
                     /translation="MPTISPIHRTPSIRKEDVQKKWYVVDAEGKTLGRLCSAIAARLR
                     GKHKPTFTPNQDCGDNIIVINAEKIKVTGRKTEQKIYRHHSRFPGGLSEIKFRDLVKE
                     NPVRIITEGVKGMLPKTKLGNKMITHCRVFAGPEHDLAAQKPVKLEL"
     gene            1375..1776
                     /gene="rpsI"
                     /locus_tag="PK683_10830"
     CDS             1375..1776
                     /gene="rpsI"
                     /locus_tag="PK683_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNH08988.1"
                     /translation="MAPKGKETWTVGRRKTAVARLKMTEGSGKVSVNGMAIEKYISLG
                     ASKVAEALSPLKVLNLQNKYDLLFNVKGGGPNGQVGAIRHALARALAKTVADGRTPLK
                     KEGFLTRDSRMVERKKYGRHKARRGTQFSKR"
     gene            complement(1773..>2037)
                     /locus_tag="PK683_10835"
     CDS             complement(1773..>2037)
                     /locus_tag="PK683_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08989.1"
                     /translation="LNPKISSKDFWKLLGIEGFNPVNNGADKYTIVKTMEELKITDPN
                     KLLTIFDVQDKNWVVKGRSGKDRNLSYIFSDLAVKFSEKRDKD"
BASE COUNT          662 a          417 c          386 g          572 t
ORIGIN      
        1 gcgtaaggtg agttatccag taaaaatggt ctgctgagca gataagcagc cttcactttc
       61 acttaaaaag agtgcaaata aaaaagctga aaatttatat gctgtcccga tgaattctga
      121 caagctgatt ttctaggcct ttatctgaga ctgcatcatg aattttcttt gctatccgtg
      181 cagcatcggg atcatccgtg cagacaataa ttcctgaatg ctctgatttc gaattatgca
      241 gctgaataaa atctcttctg ttaaatgtga taagaattct attttcactg atcgaaaatt
      301 ccagaacttt ttcatcagga atagattgat tggcatttcc tgcctcaaga cttgtcaaga
      361 cgtcattacc cagttttctc agttccagaa ctattgataa tcgaaaattc tcattggtgt
      421 aaaatcttgc catcaggccc tcaggttttc ttcaatatcc ttttcgatct cttctttatg
      481 cagaacagaa tacgaccagg cattgactaa atcggccgcc cgaagagaag gccagtcgaa
      541 tagaagctgt gcttcagaaa gaccgtttct tctggcaagc tcaagtgtcc agacaggaat
      601 tctagttctg actatgcagg ctgacccgcc ggacactccg tccttttttt caattcctgg
      661 aaaactttta gacagatcgt cggccagcag tttaaagagc agtgcttttt ccctggaact
      721 catttcagct atgtcctttt ccatttcaat gaatgtcata aggatagtgt aaagaatctt
      781 tgaaaaaatg caagtaaaga ctgcaaatgg tgaaaaacct catctcccca cctcccttcc
      841 cctttttaca ttttcgaata attcactgga cataaaaaca gactgtaaaa acatggtcaa
      901 caaaagggat tcactatgcc tacaatttca cctatacata gaacaccttc tatcagaaaa
      961 gaagacgtcc agaaaaaatg gtatgtcgtt gatgcggaag gaaagacatt gggcagactc
     1021 tgttcagcca tagcagcgag actcagaggc aagcataagc ctacatttac accgaaccag
     1081 gattgcggtg acaatatcat tgtaattaat gccgagaaga ttaaagttac aggaagaaaa
     1141 actgaacaga aaatttatcg tcatcactcc cgtttccccg ggggactcag tgaaattaaa
     1201 tttcgtgatt tagtaaagga aaatcctgtc cgaatcatta cagaaggggt caagggaatg
     1261 cttccaaaga caaaacttgg aaacaaaatg atcactcatt gcagagtttt tgcaggacca
     1321 gaacatgatc tcgcagctca gaaaccagtg aaattagaat tataaggaaa tatcatggca
     1381 ccaaaaggca aagaaacttg gactgtaggc agaagaaaaa ctgcagttgc cagactgaaa
     1441 atgactgagg gatccggaaa ggtctctgta aacggtatgg caatagaaaa atatatctca
     1501 ttaggtgcat ctaaagtagc tgaggcactt tcgcccctga aagtattgaa cctccagaac
     1561 aaatatgatc tgctcttcaa tgtaaaaggc ggcggtccaa acggacaggt cggtgcaatc
     1621 agacatgcac ttgcaagagc acttgcaaaa actgttgctg acggaagaac tcctctgaag
     1681 aaagaaggtt tcctcacaag agattccaga atggtagaac gtaaaaagta tggccgtcac
     1741 aaagccagaa gaggaactca gttctccaaa cgttaatctt tgtctctttt ttctgaaaat
     1801 tttactgcta aatccgaaaa aatataactt aaatttctat ccttaccaga acgacctttt
     1861 actacccaat ttttatcttg gacatcaaat attgtcaata atttattggg gtcagttatt
     1921 tttagttctt ccatagtctt aactatggta tatttatctg ctccattatt cacaggattg
     1981 aatccctcta ttcctaataa tttccaaaaa tctttagagg aaatttttgg gtttaat
//
LOCUS       DAOQGJ010000670         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37677_length_2036_cov_3.073700, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000670 DAOQGJ010000000
VERSION     DAOQGJ010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37677_length_2036_cov_3.073700"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK683_10840"
     CDS             complement(<1..396)
                     /locus_tag="PK683_10840"
                     /inference="COORDINATES: protein motif:HMM:NF020035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08990.1"
                     /translation="MLSQIDLSQCEKEKIHIPNLIQPHGFIITFHPDSLTVMRYSSNL
                     IQTQVGSDRNLTGRNIKELFPETLWKTVEKNLDFSNYLRHIIYEVDFPQISDQKVDIM
                     VCSARREVILELIPVYPEKDPLYSLDFQLN"
     gene            complement(380..976)
                     /locus_tag="PK683_10845"
     CDS             complement(380..976)
                     /locus_tag="PK683_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HNH08991.1"
                     /translation="MSNPIHKRLKKLTKPFHEELENCVFPQKLVNGKMTQGEYKDYLR
                     VMHQFHKQTEPFFLEYSEWKDYGIDIHSRLRTQFLEEDLKNLGVSNLPDPKPGLTVKK
                     PANFAESAGMLYVLEGSTMGGMILSKKANELFFDRLEGSCSRYFSAYRKETALKWQEY
                     CSFLNHYHISHPENEDDMVSGACTCFMTVKRFLDALSD"
     gene            1301..1630
                     /locus_tag="PK683_10850"
     CDS             1301..1630
                     /locus_tag="PK683_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08992.1"
                     /translation="MKQLILFLLGILFIQAKTPDFTKMKHLYLFKSKNYTCHPAENVE
                     GVNMRPEEILKKHSACFYVERKNETKSFSLNCSKDKTIHSSLVYSKSRDVCESVAKSL
                     KKAFAKK"
     gene            1617..>2036
                     /locus_tag="PK683_10855"
     CDS             1617..>2036
                     /locus_tag="PK683_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH08993.1"
                     /translation="MPKSKILIVEDDDSLGATLKERLSRESYLARLVNTVQKAYSEIQ
                     EFRPDLIILDVGLPDGNGFEIAREIIRYKEHPPFLFLTANAGAKERLEGFEIGAEEFI
                     PKPFHLKELLIRVRHVLETHQKKRTELKTEDVFFGNCR"
BASE COUNT          591 a          403 c          412 g          630 t
ORIGIN      
        1 gttcagctga aaatcgagag agtacagagg gtctttttca ggatagactg gaatgagttc
       61 cagaatgact tctcttcttg ctgaacagac cattatatcc actttctgat ctgaaatctg
      121 aggaaaatca acttcatata ttatatgtcg cagataattg gaaaaatcaa gattcttttc
      181 aactgtcttc cataatgttt ccgggaataa ttcctttata tttctgccgg tcagattcct
      241 gtctgatccg acctgagtct gaatcagatt gctgctgtac cgcatgacgg tcagactgtc
      301 cggatgaaag gttatgataa atccgtgagg ctggataaga ttaggaatgt gaattttttc
      361 tttttcacac tgagacagat caatctgaga gagcatctaa aaacctcttc actgtcataa
      421 agcaggtaca ggctccgctg accatatcgt cttcgttctc aggatgtgat atatgataat
      481 gattcaggaa actgcagtat tcctgccatt taagtgcagt ttctttcctg taggcggaaa
      541 aatatctgct gcaggaccct tccagccggt caaagaaaag ctcatttgct tttttggaaa
      601 gaatcattcc tcccatagtg gaaccttcca gtacgtacag catccctgcg gattctgcaa
      661 aattggcggg ttttttgact gtaagtccgg gtttagggtc gggaagattc gatactccca
      721 gatttttcag atcttcctct aaaaactgag ttctcagcct ggagtgaata tctattccgt
      781 aatctttcca ttcagaatac tcaagaaaaa agggttccgt ctgtttatga aactgatgca
      841 tcactctcag gtagtctttg tattctccct gagtcatttt tccatttacc aatttttgag
      901 gaaaaacaca attttccagc tcttcatgaa acggtttcgt aagctttttt aatcttttgt
      961 ggataggatt actcataact ggctgattct gcttttacga ttctttacct gattctgtac
     1021 acaatccttt tttaaaaaaa gtgttcactc atgtcccaaa ataggctctt cagcggctgc
     1081 tcattgttgt tttcttttca attcctgaga caattcgatc tgctcgctga aaaactgatg
     1141 cttactgtca actttttccg aactgtgaga gcagtcaggg ctgatttcaa ttttcctgct
     1201 gtatttttat cagcagtatc tgcagacacc gaaggtaaac aaacttgcca aactgtaaat
     1261 aattttcaaa gctggaataa acggttatcg gagacagatt atgaagcagc tgattttatt
     1321 tttactggga attttattca ttcaggccaa aactccggat ttcaccaaga tgaagcatct
     1381 gtatcttttt aaatcaaaaa actatacatg ccatcctgca gaaaatgtgg agggagtgaa
     1441 tatgagaccg gaagagattt tgaaaaaaca cagtgcctgt ttttatgtgg aaagaaaaaa
     1501 tgagacaaaa tctttcagcc tgaattgttc aaaggacaag actattcatt cttctctggt
     1561 ttattcaaag agcagggatg tatgcgaatc tgttgcaaaa tcactgaaaa aagcctttgc
     1621 caaaaagtaa gattctcatt gtggaagatg atgattctct gggagccact cttaaggaaa
     1681 ggctttccag ggaatcctat cttgcaaggc ttgtaaatac agtacagaaa gcctattctg
     1741 aaattcagga gtttcgtccc gacctgatta ttctcgatgt cggtctccct gacggaaacg
     1801 gctttgaaat tgcaagggaa atcatccggt acaaggaaca tcctccattc ctgtttctta
     1861 ctgcaaatgc gggtgcaaag gaaagactcg aagggtttga gatcggggca gaggagttta
     1921 tacctaaacc ctttcatctg aaagaactcc tgattcgtgt acgccacgtt ctggaaactc
     1981 atcagaaaaa aagaacagag ttgaagactg aagatgtctt ttttgggaac tgcaga
//
LOCUS       DAOQGJ010000671         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37719_length_2035_cov_2.511111, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000671 DAOQGJ010000000
VERSION     DAOQGJ010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37719_length_2035_cov_2.511111"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK683_10860"
     CDS             complement(<1..539)
                     /locus_tag="PK683_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08994.1"
                     /translation="MKQKEGKFFSGGKTFQKEKINYMHPALSPDGNTLYFACNDPEGW
                     GGYDLWSVQRSNRSESGWDEPKLLSRNINTPGNEVFPAFSADTLYFASDGLQGMGGLD
                     IFRTYKSDRNAWAPPHNLKPPVNSGADDFGFIALPVPKREGPAKPGDLLRAGFFTSNR
                     PAGSRGGDDIYRFEQRVPPP"
     gene            486..2035
                     /locus_tag="PK683_10865"
                     /pseudo
     CDS             486..2035
                     /locus_tag="PK683_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004476484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
BASE COUNT          475 a          445 c          576 g          539 t
ORIGIN      
        1 ggcggaggta cgcgttgctc gaagcggtag atgtcgtcgc cgccacggct gcctgcaggc
       61 cgattggagg taaagaaacc ggcgcgcagc agatcgcccg gtttggccgg cccttcgcgt
      121 ttggggaccg gcagggcgat aaacccgaaa tcgtcggcgc cgctgttcac cggcggcttg
      181 aggttgtgcg gcggcgccca ggcattgcgg tcggatttgt aggttcggaa aatatccaac
      241 ccgcccatgc cttgcaggcc gtcggaggca aaatacaggg tatcggcgct gaaggccgga
      301 aaaacctcgt tgccgggggt attgatgttc cggctcagca gtttgggctc gtcccagccg
      361 ctctcgctgc ggttgctgcg ctgtaccgac cacaggtcgt agccgcccca gccttccggg
      421 tcgttgcagg cgaagtacag cgtattgccg tcgggcgaca gcgcggggtg catatagttg
      481 atcttttctt tctggaaggt ctttcctccg gaaaagaatt tgccttcttt ctgtttcaag
      541 tcgcttttat gggaacagct gccacgattg tttcaggagc tgttgccgag aggatcaaat
      601 tttctgcata tctcattgtc tcctttctga tttcagttct gatctatccg gtattcggac
      661 actgggcctg gggcggggga tggctgggga aaatgggctt tgccgacttt gccggttcga
      721 ctgtcgtgca ttcacttgga ggatggatct ctctttcagg agtcattctg ctcggagcaa
      781 ggaaagacaa attcaatgaa aacggaacac ctagaaaaat acagggtcat aatcttacct
      841 tctcagtact tggagttttc atactctggt tcggctggtt tggattcaac ggagggagca
      901 cacttgcatt cacagatgct gtgcctctga tcatagtcaa tacaatgctg gctgcaagcg
      961 caggtggcct tctggccatc ttcattgcct ggacgattca cggaatttct tccgtagaag
     1021 actgtatgaa cggagttctg ggagggcttg ttgcagtgac agcaggctgc aatacaatca
     1081 ctccgataca gtcggctgtg ctgggagcag cggccgggat ctctgttgtg tatgcggcct
     1141 gggttctgga gcactggttt catttggatg atgtagtgag tgcatttcca gttcacggcg
     1201 tatgcggaat gctgggaact cttgcagttc cggtattttc aaaagatcct gcaatacagt
     1261 ttatcccgca gttgacagga acagctgtct gtgctgtctg ggcgttcgga ttgggattca
     1321 ttattttttg gttattaaaa aatacaatag ggatacgtgt cggacaggag atggaagaca
     1381 ctggtttgaa tatttcagag catggttccg gttcgagctg gatagatctg attcattctc
     1441 tcaaggatct tgcaaagggt gaaggtgatc tgacaaaaca gattcatgtc gatcagggga
     1501 cagaggcagg agctgctgct tttttaatga ataaatatct cctgagtctg ggcgaaatga
     1561 tttataccat caaggaaaaa tcacttactt tggacagctc tgctctggaa atttctgctt
     1621 catgggaggt aatgaatcaa ggaattcaga aagaaacggc gagtctggag gagatatcgg
     1681 ccatctttga tacattcaag gaatcatttc atcagatatc cgattctgct tcagaacagc
     1741 aggaaattga aagtcaggca catacccttc tttctgatct ggtttccgga ttcaagaaca
     1801 tagacactta tctgggaaac aatctgaaaa aatcgcagac atcactccgt aaaattgaag
     1861 caggcagaca ggagctggaa catcttgttt caggaatgca gaatataggt gtttctgcaa
     1921 aaaatgtgga acatctggtc aggattatga atgatatttc ccggagactg ggaatgcttt
     1981 ccatcaatgc ttcgattgag gctgcgagat caggtgatgc cgggagaggg ttcgg
//
LOCUS       DAOQGJ010000672         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37767_length_2033_cov_3.759858, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000672 DAOQGJ010000000
VERSION     DAOQGJ010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37767_length_2033_cov_3.759858"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..879
                     /locus_tag="PK683_10870"
     CDS             295..879
                     /locus_tag="PK683_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08995.1"
                     /translation="MSDIKSNFTDNEWNYIAIAPISAACAVVFADNVLTNPLSAIKEM
                     KGLKDVLKKVSAEYPSNKLIQDTFKILDDAKTLEDLNRTADRMNEDVEKNAPELKKYF
                     ENDSIEKIFEETFEKEGFLKTFSAVFAIIPMLAGKIAESKKMEGTEEFKQAIYKVAYE
                     TANAAKEGWFGFGDPISKDEARFLDKLKKFLEIA"
     gene            complement(885..>2033)
                     /locus_tag="PK683_10875"
     CDS             complement(885..>2033)
                     /locus_tag="PK683_10875"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNH08996.1"
                     /translation="PPSAPVQIPAPGITYTGSPYSFVRFDNIQVTPTVTGTVTSCTVS
                     PSLPAGLSLNSSTCVISGSPAAAQSAVSYSIKAANSSNSTTVSISIAVTTAIIYAGLA
                     GGLSISSNGGTSYTNYTTANGLGNNTVFGVYVSGSNVYAATAGGLSISSNGGTSYTNY
                     TTAAGLGNNMVNGVYASGSNVYAATNGGLSISTNGGTSYVNRTTGNGLGSNTVYAVYA
                     SGSSIYAATTGGLSISTDGGSSFVNKTTANGLGSNAAVGIHAVGSNVYVSTPVGVSIS
                     TNGGSSFTNYTSGLGSTNILGAYASGSKVYAATNGGGIGVSTDGGISYTAYNNANSSL
                     MNDSVYGIYAIGNNVYAATSSGMSVSVNGGINFTANFSAASVNAIIAQ"
BASE COUNT          599 a          402 c          469 g          563 t
ORIGIN      
        1 ggtacatagt ttcggtgata gactcctccg cctttgataa atttcaaagg atgagcataa
       61 tccggagagg ggtttgtcac ttctccgatt ccaagtgaac ttgaaacaat attgatgccc
      121 gtacaggatg ataatgacag gacgcacgcg aaaagcttta attgagtcat ggatttcatg
      181 gattgtcata attcttatat attttgttct ggcaatcaaa aatgaaaaaa tattctttac
      241 tgcaataaca gtccatactt gacaaagtct cagtaccgtt taaaactgcc ggatatgagt
      301 gatatcaaat caaactttac cgataatgaa tggaactata ttgcaatagc tccgatttca
      361 gctgcatgtg ctgtagtatt tgcggacaat gttctcacca accctctttc tgcgattaaa
      421 gaaatgaaag gactgaagga tgttctgaaa aaagtatctg ccgagtatcc ttccaataaa
      481 ctgattcagg atacattcaa aatcctggac gatgcaaaaa cactggaaga tctgaacaga
      541 actgcagaca ggatgaatga agatgtggag aaaaatgctc ctgagctgaa gaaatatttt
      601 gagaatgatt ctattgaaaa gatattcgaa gagacattcg aaaaagaagg ttttctgaaa
      661 acattctctg cagtgtttgc aataattcct atgcttgcag gtaaaatagc agaaagcaaa
      721 aaaatggaag gaactgaaga atttaagcaa gcaatttata aagttgctta tgaaactgca
      781 aatgctgcaa aggaaggctg gttcggtttc ggcgatccga tcagcaagga tgaggcaagg
      841 tttctggata aactgaaaaa atttctggag attgcatagt ttgattactg ggcaatgatt
      901 gcgtttacgc tggcagcact gaaatttgca gtgaaattga ttcctccgtt taccgagaca
      961 gacattccgg atgaagttgc tgcgtataca ttatttccta ttgcataaat accgtataca
     1021 gaatcattca tcaatgagct gtttgcatta ttatatgccg tatacgaaat tcctccatct
     1081 gtggaaacgc ctatcccgcc tccgtttgtg gcagcgtaaa ccttggaccc cgatgcgtat
     1141 gcccccagta tattcgtgct tcccagtccg gatgtataat ttgtaaaact gcttcctcca
     1201 ttcgttgaaa tggatacacc gacaggggtt gagacatata catttgaacc gactgcatga
     1261 attccgacag cagcattgct tcccagtccg ttggcggtag ttttgtttac aaaactgctt
     1321 cccccgtctg tggaaatgga aagaccgcct gtggtggcgg cataaatgct ggaaccggaa
     1381 gcatagactg catacactgt attgcttcca agaccatttc ccgttgtgcg gtttacataa
     1441 cttgttcctc cgttggtgga gattgataga ccgccatttg tagctgcata tacattagaa
     1501 ccggaggcat agacaccgtt gaccatattg tttcccaagc ctgctgcagt agtataattt
     1561 gtgtagctcg ttcctccgtt tgaggatatg gataaaccgc ccgctgtggc tgcatacaca
     1621 ttcgaaccgg atacatatac tccaaaaaca gtattgtttc ccagaccatt ggcagtagta
     1681 taatttgtat aacttgttcc tccgttagaa gaaatggaaa gtcctccggc caggcctgcg
     1741 taaatgatcg cagtggttac tgctatactg atgctgactg ttgtgctgtt tgaagaattg
     1801 gctgctttta ttgaatagga gacggcgctt tgggctgcag caggagatcc ggagatcaca
     1861 caggtactgc tgttcagact gagacctgca gggagagaag gcgaaacagt gcaggaagtg
     1921 acggttcctg taacagtcgg agtgacttga atattgtcaa accgtacaaa cgaataaggg
     1981 cttccggtgt aagttatgcc tggggcagga atctgtacag gcgctgaagg agg
//
LOCUS       DAOQGJ010000673         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37897_length_2029_cov_3.233536, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000673 DAOQGJ010000000
VERSION     DAOQGJ010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37897_length_2029_cov_3.233536"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK683_10880"
     CDS             <1..396
                     /locus_tag="PK683_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08997.1"
                     /translation="SAAVIKKYLEIIQNKGIGFYLWDDRAYKVYDRRLLNNFLFIEKC
                     PDCGAKIGRRYPLIMDRIPCCDHCGNPFDLKYWNELKLNTVKNIAWQNLETYRLQMNS
                     KKELNTVLLIVLFTFFWPAGICYLISNSD"
     gene            complement(393..>2029)
                     /locus_tag="PK683_10885"
     CDS             complement(393..>2029)
                     /locus_tag="PK683_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNH08998.1"
                     /translation="AVRAQRYFRKTEHSGRISSWEWAEIQRFGYPENREISYDYENII
                     RTTLCVEPRGGNLYVFIPPLARLEEYLELISCIELASAMLEYPVMIEGYLPPYDSRIN
                     KFMITPDPGVIEVNIHPASDFNELVRNTEILYEEARNAKLSTEKFMMDGRHTGTGGGN
                     HVTLGAAYPSDSPFLRRPDLLKSLLAYWQNHPSLSYLFSGLFIGPTSQAPRIDEARND
                     SLNELEIAFQQVKRGNYTPLWMVDRIFRNILTDVTGNTHRAEFSIDKLYSPDGAQGRL
                     GLLEMRALEMPPHYRMSIAQMLLVRSLVSRFWKNPYERKLVRFGTELHDKYMLPHYVW
                     ADFKDVIYDLNRNGYEFSEEWFEVFRNFRFPLLGVLNSNDIRLELRTAIEPWHVMGEE
                     GTSGGTARYVDSSVERIEVKVFGLTDTRHIVLCNGRPIPLRPTGVNGEFAAGVRFKAW
                     QPYSALHPHIGVHGPLVFDIYDTWNEQSVGGCTYHISHPGGRSYDTFPVNGNEAESRR
                     ISRFFTYGHTPGRFTVPPVYTDPEFPYTLDLRKNPV"
BASE COUNT          552 a          478 c          444 g          555 t
ORIGIN      
        1 tcggcggccg tcattaaaaa atatttagaa atcattcaaa ataaaggaat cggattctat
       61 ctctgggatg acagagcata caaggtgtac gacagaagac ttctgaacaa ttttctgttc
      121 attgaaaaat gtccggactg cggagccaaa atcggcagaa gatatccttt aattatggac
      181 agaataccct gctgcgatca ctgcggcaat ccgttcgacc tgaaatactg gaacgaactt
      241 aagctgaata cagtaaaaaa catagcctgg cagaatcttg aaacctacag gcttcagatg
      301 aactccaaaa aagaactgaa tacagtcctg ctcattgttc tttttacgtt cttctggcct
      361 gcaggcatct gctatctgat ttccaactcc gattaaacag gattcttcct gagatcgaga
      421 gtatacggaa actccggatc agtgtaaacc ggcggcactg taaatcttcc cggcgtatgt
      481 ccgtatgtaa agaatctgga tatccttctg gattcagcct cattcccgtt taccgggaat
      541 gtatcataac ttcttcctcc gggatgagag atatgataag tacatcctcc gacggactgc
      601 tcattccagg tatcataaat atcgaaaaca agggggccat gcaccccaat atgaggatga
      661 agtgcagaat acggctgcca tgccttgaat ctgactccgg cagcaaactc tccgttgact
      721 cctgtaggcc tgaggggaat cggtcttcca ttgcagagca caatatgtct tgtgtcggtc
      781 agtccgaaca ccttgacttc aatgcgctcg acggatgaat ccacataccg tgctgtcccg
      841 cccgaagttc cttcctcacc cattacatgc caaggttcaa ttgcagtccg gagctccaga
      901 cggatgtcat tggaattcag cacacccaga agagggaaac ggaaattgcg gaatacctca
      961 aaccactctt cggaaaattc atatccgttt ctgttcagat cataaatcac atccttgaag
     1021 tcggcccaga cataatgggg aagcatgtat ttatcatgaa gttctgtgcc gaaccggaca
     1081 agttttcttt catacggatt cttccagaaa cgggatacta gcgatctgac aagaagcatc
     1141 tgtgcaatgc tcattctgta atgaggaggc atctcaagcg ctctcatttc aagaagcccc
     1201 agtctgccct gtgcaccgtc cggggagtac agcttgtcta tgctgaattc tgccctgtga
     1261 gtgtttcctg tcacatccgt gagaatgttt ctgaaaattc tgtcaaccat ccagagaggg
     1321 gtataatttc ctcttttgac ctgctggaat gcaatttcaa gttcgttgag actgtcattt
     1381 cttgcttcat caattcgcgg tgcctggctt gtaggtccga tgaacagtcc ggagaaaaga
     1441 taggaaagac tcggatgatt ctgccagtat gcaagcagac ttttcagaag atcaggtctc
     1501 ctgagaaaag gagaatcaga aggatatgca gcacccagag tgacatggtt tcctccccct
     1561 gttccggtat gccgtccgtc catcataaat ttttcggtgg acagtttggc atttcttgct
     1621 tcctcataaa gtatttcggt attgcggaca agttcattga aatcggatgc aggatggatg
     1681 ttcacttcaa tcactccagg atcaggagta atcataaact tgtttatccg actgtcatat
     1741 ggcggaagat atccttctat cattacaggg tattccagca ttgcactcgc aagttcaatg
     1801 caggaaatca gttctaggta ttcttcaagt ctggcaagag gaggaatgaa cacatagaga
     1861 tttcctcctc tcggttcaac gcagagagtt gtacggataa tattttcata atcataggat
     1921 atttccctgt tctcaggata tccgaatctc tgaatttccg cccactccca tgaactgatt
     1981 cggcctgagt gttctgtctt tctgaaatat ctctgtgctc tgaccgcag
//
LOCUS       DAOQGJ010000674         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37902_length_2029_cov_2.660081, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000674 DAOQGJ010000000
VERSION     DAOQGJ010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37902_length_2029_cov_2.660081"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PK683_10890"
     CDS             complement(<1..1444)
                     /locus_tag="PK683_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH08999.1"
                     /translation="MRKIIHNHLLLVLLAILTIFHITLRTDNSFTSDALLKAIQTDSF
                     VSNGFKSFSLHPGSEKIDSTFETFFFSGSFAVQTKTGLFSVFPDMLAAIASVLVLLRI
                     PYMYFPFIFSVPFAFAVFFLFKKKLISAENGIIMLVLTVLFPLISEFSENALFFLINT
                     FGFYFWIKFRENDSLKFLFFSVFTVSLTVWLRLEAILFLISLAAGEFFSGRKKIEIPW
                     LRKFLPFFLAGTIPLLLLLIWNSHYYGHPFGLRYIYNYIENKVTAGDRTVNLLSTVFF
                     FYDNGIPKIGILLHSVFLLIPILFFLKKKEGTQEIKFLLWTSFLFLLSVGLSAPNNGV
                     TVNGRYHILALLPLFRLYQLWDDETAGKKRKAVLIGCLAVSSLISFTAVLVLKYAFNV
                     QKDIRVFHKSVKSDLHVFTNSFLCNAAGLDHLEMTVICLKKGLDLKKIADNIVRNPEI
                     KTVSFLNYDENKNKPFIKSEMALSAEKDEEF"
     gene            complement(1482..>2029)
                     /locus_tag="PK683_10895"
     CDS             complement(1482..>2029)
                     /locus_tag="PK683_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNH09000.1"
                     /translation="CDCVNIGLHGNVFPKIDAVVSGINRGVNMGHDVHYSGTVGAARH
                     ASIHGILSFSVSSGRFGKDVSYQREAEAFFEIFENLHSVFQPGCVYNINFPPNFDSVE
                     KSLRAVSLGKRIYHDTYEVTEFVDGIYEFYLGGSELGNDLTPETDFEAYSKGCVTLTP
                     IELNTTDFDRLEKLKESLNNH"
BASE COUNT          676 a          392 c          389 g          572 t
ORIGIN      
        1 aaaactcttc atctttttca gccgacagtg ccatttcaga ttttatgaag ggcttgttct
       61 tgttttcgtc ataatttaaa aagcttacag tttttatttc aggatttcta actatattgt
      121 ctgcaatctt tttcagatcc aaaccttttt tcagacagat tacagtcatt tccagatgat
      181 caaggccggc cgcattgcag agaaaagaat tagtgaatac gtgcaagtct gatttcactg
      241 atttatgaaa aacacgaatg tctttctgaa cgttaaatgc atatttcaga accaggacag
      301 cagtgaaaga gattaaggaa gacacagcta gacatccaat taggacagcc tttctcttct
      361 ttcctgcagt ttcatcgtcc cagagctgat atagtctgaa aaggggaagg agagccagaa
      421 tatgatacct tccattcaca gtaactccgt tattgggtgc agacaagcct acagataaga
      481 gaaaaaggaa tgatgtccac agaagaaatt ttatttcctg agtgccttcc ttttttttaa
      541 ggaaaaataa gattggaatg agaagaaaga cagagtgaag cagtattcct atctttggaa
      601 tgccgttgtc ataaaaaaag aatactgtgg acagcaggtt cactgtgcgg tctcctgccg
      661 taaccttgtt ttcaatgtaa ttatagatat accgaagtcc aaaaggatgt ccgtaataat
      721 ggctgttcca aatcagaaga agaaggagcg gaattgtccc tgccagaaaa aacggcaaaa
      781 attttcgaag ccagggaatt tctatttttt ttctccctga aaaaaactcg cctgcagcca
      841 gtgaaattaa aaagagaatt gcttcaagtc tcagccagac agtaagtgaa acagtaaaga
      901 cagaaaaaaa taaaaacttt aagctgtcat tttctctgaa ttttatccaa aagtaaaacc
      961 caaatgtatt gatcaggaag aacagcgcat tttcactgaa ctcgcttatc agaggaaata
     1021 gaacagtaag aaccagcatt attatgccgt tttctgccga gatgagcttt ttcttgaata
     1081 ggaaaaaaac tgcaaatgca aaagggacag aaaaaataaa tggaaaatac atatagggta
     1141 ttctcagcag gaccaggacg gaggcaattg ctgccagcat atcaggaaat acactgaaca
     1201 gaccggtctt tgtctgtact gcaaatgagc cgctgaaaaa aaatgtttca aatgtgctgt
     1261 caattttttc cgatccaggg tgaagactga aagacttaaa tccgtttgaa acaaaagaat
     1321 ctgtctgaat tgctttcaga agagcatcag aagtaaagga attgtctgtt ctgagagtaa
     1381 tgtgaaatat tgtcagtatt gccaataata ccagaagcag atgattgtga ataatttttc
     1441 tcaaaaagtc ctcctctatt tcagaaatgc attctgaatg atcagtgatt atttaatgat
     1501 tctttcagtt tttcaagccg atcaaaatca gtagtattca gttcaatggg tgtaagtgtt
     1561 acacagccct tagaataagc ttcaaaatct gtctccggag tcagatcatt gcctagttct
     1621 gaacccccca gataaaattc atatattcca tccacaaatt ctgtcacttc ataagtgtca
     1681 tggtagattc gtttccctaa tgacaccgca cgaagtgact tctcgacaga atcaaaattc
     1741 gggggaaaat ttatgttgta gacacatcct ggctgaaaaa cagaatgcag attttcaaaa
     1801 atctcgaaaa acgcttctgc ttctctctga tagctcacat cttttccaaa tcgtcctgaa
     1861 cttactgaaa aagaaaggat gccgtgaatc gaagcatgtc ttgctgcacc tactgtcccg
     1921 gaataatgaa cgtcatgacc catattcaca cccctgttga tgccggatac gactgcatca
     1981 attttcggaa aaacgtttcc gtgcagtcca atattcacac agtcgcagg
//
LOCUS       DAOQGJ010000675         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37926_length_2028_cov_3.034465, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000675 DAOQGJ010000000
VERSION     DAOQGJ010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37926_length_2028_cov_3.034465"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..546)
                     /locus_tag="PK683_10900"
     CDS             complement(82..546)
                     /locus_tag="PK683_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09001.1"
                     /translation="MKQIYSFLQLLILAAAFSFCGKPPEPGIPVTLDKVCSAEYNPFF
                     DEKKRQVHRRLSFEGYPAFPRSVMVSSTVILDMYPSPDRKGTPVSVSFSVKASRNSVE
                     KPGKGYTDKDLKINANDGTEVGFNTKIRVHGRRLGEANSGCVVTVDLIETVK"
     gene            complement(596..>2028)
                     /locus_tag="PK683_10905"
     CDS             complement(596..>2028)
                     /locus_tag="PK683_10905"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH09002.1"
                     /translation="MTVGPAGIDVINRKNSKLYEFLDNVNRKEPVKNSAPERDRLSKK
                     KEKKEEIRIADALEGDADEDDAEESISKADLGKSSKDQPADPAKIQEADKSPGRLSDT
                     AGPAKSPDKGGADSKVSGETSVPPPSERPKTAVSSDEKPEQVRSAERERRRPVSETTA
                     VSRPRPDPEPEEKREKVEPYTGNRKKFQNLIDKRDYKQALQFALDWRTGNLQDTLALT
                     ALGDAFLKNGNRKDAVRAYSSLIDYFPKRADIRRWAGEKLLSMGEYEEASDTLRHALI
                     QRPDHPSTYHLLASAYIKGGKYAEAASVILKGISFKFDSRFSSVHDILYDDLDLAYSL
                     ALKNGSRDMEYFEKIKTEYKLKVLKKEIRFILVWETDANDVDFHIYDRKGNHAWYSSK
                     QLESGGELYADLTGGYGPECFRIINPSAFPYRLEAHYYSRGPMGFGMGAVQIIRYDGE
                     KELELETRDFVIMNDGAYLDLGKVKE"
BASE COUNT          477 a          496 c          393 g          662 t
ORIGIN      
        1 aaagagaatc cctgtttatt ccgagttttt ttttatcgtt aaagtattaa aaaaaccgga
       61 aaaacaggga acctggcttg tttattttac tgtctctatc agatcaaccg taactacaca
      121 gcctgaattt gcctctccaa gtcttctgcc gtgtacacgt atttttgtat tgaatcccac
      181 ttctgtgccg tcattggcat taattttcag atccttgtca gtgtaccctt tacccggttt
      241 ttccacactg ttcctgctgg cctttacgct gaaagaaact gatacaggag ttcctttcct
      301 gtctggtgaa gggtacatat cgagtatgac tgtagaactc accattactg atctcggaaa
      361 tgccggatag ccttcaaagg aaagccttct gtgaacctgt cttttctttt catcaaaaaa
      421 aggattgtat tcggcagaac agactttgtc caatgtcacg ggtatgcccg gctcaggcgg
      481 ctttccgcag aatgaaaaag ctgctgccaa aatcagcagc tgaagaaatg aataaatttg
      541 tttcatagct gcattctaat tgagattgag gattgtaaac tttttttcag aaagttcact
      601 cctttacctt tcccaaatcg agataggcac cgtcattcat tatcacaaaa tcccttgtct
      661 ccaattccag ctccttttca ccgtcatacc ggatgatctg aaccgctccc attccgaatc
      721 ccataggtcc tctgctgtaa taatgcgctt ccagcctgta agggaaagca gagggattaa
      781 tgatccggaa gcattcagga ccgtatccgc ctgtaagatc tgcatacagt tcaccgccgg
      841 actccagctg tttcgaactg taccaggcat gatttccctt cctgtcataa atatgaaaat
      901 ccacgtcatt ggcatctgtt tcccagacca ggataaaacg tatttctttt ttaagaactt
      961 tgagtttgta ttctgtctta attttttcga aatactccat atccctgctt ccgtttttca
     1021 gggcaagact gtatgccaga tccagatcat cataaagtat gtcatgtacc gatgaaaacc
     1081 tggaatcgaa tttgaaactg attcctttca gtatcactga ggcagcctca gcatactttc
     1141 cgccttttat gtaagcagat gcaagaagat gatatgtgct tggatgatcc ggtctctgaa
     1201 taagagcatg tctcagtgtg tcggatgcct cttcatattc tcccattgag agaagttttt
     1261 ccccggccca tcttctgata tctgctcttt tgggaaaata gtcgatcagt gatgaatagg
     1321 cacgcactgc atcttttctg tttccgtttt tcagaaatgc gtcaccgagc gcagtgagag
     1381 ccagcgtgtc ctgaagattt ccggtcctcc agtccagtgc aaactgaaga gcctgtttat
     1441 aatctctttt atcaattaaa ttctggaatt ttttcctgtt tcctgtatag ggctccactt
     1501 tttctctttt ttcctctggt tccggatcag gtcttggtct cgaaactgcc gtcgtttcag
     1561 aaacaggtct tcttctttct ctttcagcac ttcgcacctg ttccggtttt tcatctgagg
     1621 aaactgcagt tttcggtctt tctgaaggcg gaggaacaga ggtttcaccg gaaacctttg
     1681 aatctgcacc gcctttatcc ggagactttg caggaccagc tgtatcactt agtcttccgg
     1741 gagacttgtc tgcttcctga atttttgcag ggtctgccgg ctgatcctta gagctcttgc
     1801 cgagatctgc cttgcttatg gattcttccg catcatcctc atccgcatct ccttccaatg
     1861 catccgcaat tcggatctct tccttctttt ctttcttctt ggaaagtctg tctctctccg
     1921 gagcggaatt ttttacaggc tcctttcgat ttacattgtc cagaaattca tacagcttgg
     1981 aatttttccg gttgatcaca tcaattcctg ccggccctac agtcatta
//
LOCUS       DAOQGJ010000676         2027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_37961_length_2027_cov_3.145030, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000676 DAOQGJ010000000
VERSION     DAOQGJ010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37961_length_2027_cov_3.145030"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PK683_10910"
     CDS             complement(<1..499)
                     /locus_tag="PK683_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH09003.1"
                     /translation="MIQNRRTRGKIKVLVVDDQAIIRNILEKGLNSYENLEVVGKAAD
                     VFEARDMIVELDPDVISLDIEMPKMNGLEFLKKLMPQYPLPVVIVSSHTLEGRQITLD
                     ALDAGAVDFVTKPDGDSGSLNRMISELSEKLTAAAGTDLSRWKKGNKPDRVRNENRSS
                     LSGRDY"
     gene            complement(550..>2027)
                     /locus_tag="PK683_10915"
     CDS             complement(550..>2027)
                     /locus_tag="PK683_10915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNH09004.1"
                     /translation="SVILELKELEKFSEEKKQKKELKRFGLFSDPVSQSDEEIIIKKE
                     EKFGFFTESDSDVQEKNSEDSEGNSKTAAEKKQKTAVSAQSRQNENTGFHDETRDIRI
                     GTDKLDSLIDMLGELVIADAMVHRHSYSEILNVELLRKSVSQLNKITRYLQDLGLSMR
                     MIPVAGLFKKMTRIVRDLAKKSGKNVRLEIKGEETELDKIIIEQLSDPLLHIMRNAID
                     HGLETKEERESLGKDAVCIISLEAMHKGNEIWITVSDDGRGLNRTRILAKAREMNLTD
                     EDENSMSDSEVWDLIFHPGFSTAEKLTDLSGRGVGMDVVRKNIQRLKGRIDVQSIQGS
                     GTTFFLRIPLTLAIIDAMIVMYSGRYYVIPTENIDRFIDLNTIVPSDLHGKQKVLDIN
                     GNLIPLVDLNLVFDYRESGEETGSRIAAVVGIEGRFAALQLDSIIGSQQVVVKHLPES
                     MEHLQGVFGTAILGDGEVGLILDAEFIIKKYSTNKIQSAQI"
BASE COUNT          516 a          506 c          353 g          652 t
ORIGIN      
        1 aataatctct gccggacaga gagcttctgt tttcattcct gactctgtcc ggtttattcc
       61 ccttcttcca tctgctcaaa tcagtgcctg cagccgcggt cagcttttcc gaaagttcag
      121 aaatcattct gttcagactt ccggaatcac cgtccggctt tgtcacaaag tccacagctc
      181 ctgcatccag cgcatccaga gtaatctgac gcccctctaa agtatgtgaa cttactatta
      241 cgacgggaag agggtactga ggcataagtt ttttcagaaa ttccaggccg ttcattttcg
      301 gcatctcaat atccagagaa attacgtcgg ggtccaattc tacaatcata tcccttgctt
      361 caaagacatc ggcggctttt cctacgacct ccagattttc atatgaattc agtccctttt
      421 ccagaatatt tctgataata gcctgatcat caacgacaag gactttgatt tttccccttg
      481 tccgcctgtt ttgaatcatt ttgaaatcct caatccgtct ggcataattt catcctgcat
      541 caaaggtttt catatctgcg ccgactgaat tttattagta gaatattttt taatgataaa
      601 ttcagcatcc agaataagtc caacctctcc gtctcccaga attgcagttc caaaaacccc
      661 ctgcaggtgt tccatagatt cgggaaggtg ttttacgaca acctgctgtg agcctataat
      721 tgaatccagc tgcagtgctg caaaacgtcc ctcaattccc acaacggcag cgatcctgct
      781 gcccgtttct tcgccgctct ccctgtaatc aaaaacaaga ttcaagtcca caagaggaat
      841 cagatttccg tttatatcca gaaccttctg ttttccgtgc aggtcagagg gaacaattgt
      901 attcaagtca atgaacctgt ctatattttc cgtaggaatt acatagtatc tgcctgaata
      961 cattacaatc attgcatcaa tgatagcgag agtcagaggg attctcagaa aaaaagtcgt
     1021 gccggagcct tgaattgact gaacatcaat gcgtcctttg agacgctgaa tgttttttct
     1081 gaccacatcc attccgactc ctctgcctga aagatcagtc agtttttctg cagtggaaaa
     1141 gcccggatga aaaatcagat cccagacctc gctgtcagac attgaatttt cgtcttcatc
     1201 tgtcagattc atttcacgtg ctttcgcaag tattcttgtt ctgttcaggc cccttccatc
     1261 atcactgaca gtaatccaga tctcatttcc cttatgcatt gcttccaggg agattatgca
     1321 tactgcatct tttccaagac tctccctttc ttcctttgtc tcaagtccat gatcaatggc
     1381 atttctcata atatgaagaa gaggatccga aagctgctct ataatgattt tatccagctc
     1441 agtttcttcc cccttgattt ccagacggac attcttgccc gatttttttg cgaggtcacg
     1501 tacaattctt gtcatcttct taaagagtcc cgcgacaggg atcatcctca ttgaaagacc
     1561 gagatcctgc agatatcttg taattttatt cagctgtgac accgattttc tgagaagctc
     1621 cacattcaga atttctgaat acgaatgtct gtgcaccatg gcatctgcaa tgacaagctc
     1681 acccagcata tcaatgagag aatccagctt atccgttcct atgcggatgt ctctagtctc
     1741 atcatgaaaa cctgtatttt cgttctgtct gctctgagca gaaaccgcag ttttctgttt
     1801 tttctctgca gccgttttcg aattcccttc agagtcttct gagtttttct cctgaacatc
     1861 tgaatcagat tcagtaaaaa aaccgaattt ttcttctttt ttaatgataa tttcttcatc
     1921 cgactgactg actggatctg aaaaaagacc gaatctcttt aattcttttt tttgtttctt
     1981 ttcctctgaa aatttttcca gttctttcag ttccagaatg acagaat
//
LOCUS       DAOQGJ010000677         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38027_length_2025_cov_2.888832, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000677 DAOQGJ010000000
VERSION     DAOQGJ010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38027_length_2025_cov_2.888832"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PK683_10920"
     CDS             <1..1270
                     /locus_tag="PK683_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09005.1"
                     /translation="KTVFIKIFILCTVFTADCTGRNSVSGASTDSGERGEVGRQLEYA
                     AVIDAGSSGSRIRIYRIAGIGKDLIPEVTELGTEDKTLQMKIEPGISELNGRNENLSP
                     EKLGEYLNPLFASGKSILSKEGLREDQFRNVPIYFGATAGVRRLSQERQTKLMNLVSS
                     VLKDSGFLSKGALVVSGEFEGVYAWTCINSLKGTLGAKTKDTYGILEMGGASTQITFV
                     PEKGPYQSPRRFRLGEREYPLYSYSYNALGINEARALLDHSECRNPKGNYEKCKAKIA
                     LDLNADRFCGTTTACGLQGVSQPAPGGKFTALGNFANLGTDLGLTYLSSVKLDEAGKQ
                     ICRSSEEKIREMFPGLSKTPQKFLNGTCFHSALTSVMLSGNGKGYDGLGFTADSESIE
                     PVRSVNNEAPSWTKGLVIMEKTGNHTWESE"
     gene            complement(1271..1972)
                     /locus_tag="PK683_10925"
     CDS             complement(1271..1972)
                     /locus_tag="PK683_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09006.1"
                     /translation="MNLNVRTFFAVSFGAVVLLLIYMLVATPGSSSKRKRIKAERLGS
                     ALFGGGAGAGDSDSACRGCRVKAGSSVFDDDFTKVGVNFDVQEESKKDPSKGEAPIDP
                     RTGKPYDDATMEEFDRLRELFPSNELIPKRFNPDEKAAKAQAEQEYNKAIAAVSSGNP
                     SKDDVKLYYNKQIKTIKDRLEIVEYLLDALKEEGGEGDKDGQFQKIMEGTKQQLTQME
                     TQKSDAYTKFGVSGD"
BASE COUNT          552 a          469 c          478 g          526 t
ORIGIN      
        1 aaaaacagtt ttcataaaaa tttttatact ctgcactgta tttactgcgg actgcaccgg
       61 cagaaacagc gtaagcggcg caagcaccga cagcggtgaa aggggtgaag tcggcagaca
      121 gctcgaatat gcagcagtca tagatgcggg aagttccgga tcaaggatca gaatctacag
      181 gattgcaggt atcggaaaag atctcatccc tgaagtcacg gaacttggaa cagaagacaa
      241 aactcttcag atgaaaatcg aacctggaat ttcggaattg aacggcagga atgaaaatct
      301 cagtccggaa aaactgggag agtatctgaa tcctcttttt gcttcaggaa aatccattct
      361 ttccaaagaa ggcctcaggg aagatcagtt tcgaaatgtg ccgatttatt tcggtgccac
      421 tgcaggcgtc agaagactct cccaggagcg gcagacaaaa ctgatgaatc tggtttcttc
      481 cgttctgaag gattcaggtt ttctttccaa gggggcgctg gttgtttcag gagaatttga
      541 gggggtctat gcctggacct gcatcaattc actgaagggc actctgggag caaaaactaa
      601 ggacacttac ggaatcctgg agatgggcgg tgcatccaca cagatcacat ttgtgccgga
      661 gaaagggccc taccagtcgc ctagaagatt ccgtctcggc gaaagagaat accctctcta
      721 ttcctacagt tacaatgcac tcggaatcaa cgaagcaaga gcgcttctgg atcattcgga
      781 gtgcagaaat ccaaaaggca attatgagaa atgcaaggcc aagatcgcac tggatctgaa
      841 tgcagaccgt ttctgcggaa cgactacagc ctgcggactt cagggagtaa gccagccggc
      901 acctggcgga aagtttactg cactcggaaa ttttgcaaat ctgggaactg acttaggact
      961 gacgtatctg agctctgtca aactggatga ggcaggaaaa cagatctgca gaagttcaga
     1021 agaaaagatc agagaaatgt ttccaggact gtccaagact cctcaaaaat ttctgaacgg
     1081 gacctgtttt cattctgcac tcacaagcgt tatgctttcc ggaaacggaa aagggtatga
     1141 cggactgggg tttacagcag actcagaaag tattgagcct gtgagatctg taaacaatga
     1201 ggcgccgtcc tggacaaaag gacttgtcat tatggagaag accggaaatc acacttggga
     1261 atctgagtaa tcagtctccg ctcactccga acttggtata agcatctgat ttctgagttt
     1321 ccatctgtgt cagctgctgc tttgttcctt ccatgatttt ctggaactga ccgtctttgt
     1381 ctccttctcc gccctcttct ttcagtgcgt ccagaaggta ttctacaatt tcgagtctgt
     1441 ccttgattgt cttgatctgc ttattataat acagtttaac atcatccttg gatggattcc
     1501 cgctcgaaac tgctgcaatc gccttgttgt attcctgttc cgcctgagcc tttgcagctt
     1561 tttcatcagg attgaatctt ttaggaatca gctcattcga cggaaagagt tcgcggagtc
     1621 tgtcaaactc ctccatcgta gcatcatcat agggttttcc tgttcttgga tcaatcggtg
     1681 cctctccctt ggaaggatct tttttggatt cttcctgcac gtcaaaattg actcccacct
     1741 ttgtgaaatc atcatcgaac acagaacttc ctgccttcac cctgcatcct ctgcaggcag
     1801 aatcggaatc acccgcaccg gcaccgccgc cgaaaagagc agatcctagt ctctctgcct
     1861 tgattcgttt tcttttggaa gatgagccgg gtgttgccac aagcatatag atgagaagga
     1921 gcacaacagc tccgaaggat accgcaaaaa aagttctgac attcagattc atacctaatt
     1981 tagacagtgc ttcggatttc aggtaaagga tttttcagat tattc
//
LOCUS       DAOQGJ010000678         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38046_length_2024_cov_3.309802, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000678 DAOQGJ010000000
VERSION     DAOQGJ010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38046_length_2024_cov_3.309802"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK683_10930"
     CDS             complement(<1..480)
                     /locus_tag="PK683_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNH09007.1"
                     /translation="MNTYTKKDYLIMVGLILLCLVPAVAGIYRLITLGFGGEIRPENE
                     RFFHSPAPAVFHIVSSLVFGLLGAFQFSPGFRRKHLEWHRVSGQFLVFFGLVSALSGL
                     WLTLFYPHIPTDGDYLYSIRIAVGIAMTICVILGFTSIRKRQISNHSNWMIRAYAIGM
                     "
     gene            complement(1397..1645)
                     /locus_tag="PK683_10935"
     CDS             complement(1397..1645)
                     /locus_tag="PK683_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09008.1"
                     /translation="MGEYYDSLASRLDAEGYGDDGEGNITGLRDDGSIEIVFPTGDNK
                     VVRPDDSFFYLSPEHAGEEDPGESADEEEAQSEEEYTE"
     gene            1837..>2024
                     /locus_tag="PK683_10940"
     CDS             1837..>2024
                     /locus_tag="PK683_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09009.1"
                     /translation="MKISNRRDAVNKGADSNTPEFYKKEYDFMNYTESNKKMQTKEKS
                     AELIGIAYNCNSYEGKSLL"
BASE COUNT          673 a          413 c          390 g          548 t
ORIGIN      
        1 cataccgatc gcataggccc ggatcatcca gttgctgtgg tttgaaatct gccttttcct
       61 gattgaagta aagcccagaa tgacacaaat ggtcattgca attcccaccg caatacggat
      121 ggaatacaga taatcaccgt cagtcggtat atgcggataa aaaagtgtca gccataatcc
      181 ggacaatgcg gagaccagac cgaagaatac gaggaattga ccggataccc tgtgccattc
      241 gagatgtttt ctgcgaaagc ccggggaaaa ctggaacgca ccaagcagtc cgaaaacaag
      301 cgaggaaacg atatgaaaaa ctgcaggagc aggagaatga aaaaatcgtt cattttcagg
      361 tcttatctcc ccgccgaaac cgagagtgat cagccgataa atgcctgcaa ctgccggaac
      421 aagacagaga agaatcagcc cgaccatgat aagataatcc tttttggtat atgtattcat
      481 tatagctccg actgccaagt gttttcactg atgccgaaaa atccagtctg attttagatt
      541 tctgagggga aaactgactg ttttggttct ttatgacaca gtggaaattt cgtcccggac
      601 tcagtaaaaa atacagatcc cagacattat actcattttc aaaagtcttt gcgcatttat
      661 tctgtttcga ctcaatagaa ttaagcctgt cagcaagaaa aaacgcaatt gtttttgaga
      721 accagtatat attcttttta tgcaaagcag aatgagagag caagaatgat gtaattttta
      781 taaagccgga tcagatgccg gagcaatgca cggtattatc ctgatacccc ttatattttt
      841 ttattcactg ctcctcctta ggtcactccg gaaaaataag cacatcagta tttataaaaa
      901 aacggattct agatgcagcg gatatttttt catttactct tgtttttaat gcatatggag
      961 tcaaagcagc aggtctctca ggtgcagacg gacggccttc cagcagaggt aatgatttcg
     1021 acaagcgaag ccggtctcag atttgaatca ttgacagttt caaatgcagg gacttacacc
     1081 cattatcact tactggagag actgtaaaat aaacaatgaa acgctggctc ctgcgagaaa
     1141 caaggaaatc agtacagaaa acccgcagaa taaaaataag gcttgctttg ccagattagt
     1201 ttttgctgcc ggataaagtg aaaatgctga atatgcgaga gcactgtcaa aaaaggtgtt
     1261 tataaaaaag tgatgcatta tgataaaaaa tattataatg aatcaaatga aaaattcagg
     1321 gaaatgaaac gtcttctgaa ataagacaga gctcatagga attgcatata actgcaattc
     1381 ctatgaaggg aaatccttac tcagtgtact cttcttccga ctgagcttct tcttcatctg
     1441 cagattctcc cgggtcttct tccccggcat gttccggaga caaataaaag aaactgtcat
     1501 caggccgcac tactttgttg tctccggtag gaaaaacaat ttcaatgctt ccgtcatctc
     1561 taagacctgt tatatttcct tcaccgtcat ccccataacc ctctgcatcc agacggctcg
     1621 ccaatgaatc gtaatattct cccatatttt tctcctttat tatacctatc ctttgtcatt
     1681 ctgctgaaac ctttaccaaa aaacgaaaga agataaattt cactgacagt ctttttattt
     1741 attagaatct gtcaaggaat atatgcactg ctcaatatcc cgtgaaaaaa ttcattacga
     1801 gtataaagga aatcctgacg atgaattctt tacaatgtga aaatatcaaa taggcgggat
     1861 gccgtcaaca aaggagcgga cagcaatact ccagaatttt ataaaaaaga atatgatttt
     1921 atgaattata ctgaatcgaa taaaaaaatg cagacaaaag aaaagtctgc agagctcata
     1981 ggaattgcat ataactgcaa ttcctatgaa gggaaatcct tact
//
LOCUS       DAOQGJ010000679         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38055_length_2024_cov_2.773997, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000679 DAOQGJ010000000
VERSION     DAOQGJ010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38055_length_2024_cov_2.773997"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PK683_10945"
     CDS             complement(<1..451)
                     /locus_tag="PK683_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09010.1"
                     /translation="MKILTILIFFIFNCSDSSSGSSSSNLALLLRGMNSKSSSSADKL
                     STNPFSRTVPTSFAVASSTASKTLSSKAFGEGSLTKSGTDDFPADFENKKKKFQAVLE
                     KPENISDCLAAIPTQFKLSKSTPSCYGPTLGYANHPDGAGVAVPGCPP"
     gene            1141..>2024
                     /locus_tag="PK683_10950"
     CDS             1141..>2024
                     /locus_tag="PK683_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09011.1"
                     /translation="MMETMILFSGSVFSFVWGLGILAEREQNAGNRLLSCILLISGIW
                     LLSGAYAAGEIHLRFPHFYLIHLPFVYFYGPVLYSYYSRILLEEEIPLSRMHFHYAVP
                     FAVLFIILPFHFQSTEDKILFIRSAGVGQISQYHILIYAVNAGAKISILIYFTMILKK
                     NMSAFSDLKNPSSLWKKYVLLIIFLLYGALVLGLLGFVLGRFFLVKISAMLLPFILFL
                     FFILSRRHPETVSGLRQEFRKIRYGQSKIDSLNVEEIIQRLETLMKEEKAFSDEDLSL
                     PSLSEELGISVHQLSQIL"
BASE COUNT          528 a          412 c          448 g          636 t
ORIGIN      
        1 caggaggaca gccaggcaca gcgacacccg ctccatcagg atgatttgca tatccgagcg
       61 tgggtccgta gcagctgggt gtacttttgg aaagcttgaa ttgggtggga atggccgcaa
      121 gacagtcaga gatattttcc ggtttttcca atacagcctg aaattttttt ttcttatttt
      181 cgaagtcggc cggaaagtcg tcagttcctg attttgtcaa agaaccttct ccaaaagcct
      241 tggaactcag tgttttggat gcagtgcttg atgcaactgc gaaacttgtc gggactgttc
      301 tgctgaaagg atttgtactg agcttgtcgg cggaagaaga tgatttggaa ttcattcctc
      361 tcagaaggag agccaagttt gatgaagagg aaccggaact gctgtctgaa cagttaaaaa
      421 tgaaaaagat gagaatcgtt aagattttca ttaagatgta attcatgtaa tcctccgaat
      481 gcatatcagt ccggtatagt attttggaaa aaaatgtcaa ttgttttaat atgtcacccc
      541 tagtactttt actgagatca gggaacgaag cactctcctt ggcttttgat gcctgatctt
      601 tcattctgaa tcagaacagt tttccgatgc agccgaattc catatccggc ccgacaatat
      661 gaatgctttc atctcagaag gaaaggctcg tttcattatt tattgaattg cacatactgc
      721 ttactcatct tacacctgcg gccgcctgtg gctgcctgcg gcttgagggc tctgctgcaa
      781 gtctgcacgc tgcgaagcgg ctgtttttag tgattcatcg caggctgtta ctgccggatt
      841 ttcataatgt tttctgaaga atacagagtc taggcagtat atcgtatttg cgtaagttga
      901 gctgcttaaa acaaatagga tatttcataa ccgctggatg tttttggaga catctgtccg
      961 aaaaaatacg ttagcatatc atctgtccgg tcctccgatc tgcaataagg gaagtccttc
     1021 tgaagcagat aataaggaag atcactgaac attaaaaaat catcacgtag aattccgatc
     1081 caggacggaa ataagggaaa atgactggaa aaagaaagga tacgggacat tgtaaacctg
     1141 atgatggaaa caatgattct tttttccggt tctgtattca gttttgtatg ggggctcggg
     1201 attcttgcag aaagagagca gaatgcagga aacagacttc tctcctgcat tctgctcata
     1261 tcaggcatat ggcttctttc gggagcttat gctgcaggag aaattcatct gcggtttcct
     1321 catttttatc tgattcatct tcctttcgtg tatttttacg gaccagttct ttacagttat
     1381 tacagcagga ttctcctgga agaggagatt cctctcagcc ggatgcattt tcattatgct
     1441 gtaccttttg cagttctttt catcattctt ccgtttcatt ttcagagtac cgaggataaa
     1501 attctcttta tccgcagtgc aggagtcgga cagatttcgc agtaccatat tctgatctat
     1561 gcagtcaatg caggggcgaa aatttccatt ctgatatatt ttacaatgat tctgaagaag
     1621 aatatgtctg ctttttccga tcttaagaat ccgtcctctc tctggaaaaa gtatgtttta
     1681 ttgattatat ttcttctcta tggagctctc gttctcggac tcctcggttt tgttctcggc
     1741 cggttttttt tagtcaaaat cagtgcaatg cttcttccgt ttattctttt cctgtttttc
     1801 atactctccc ggcgtcatcc tgagacagta tccggtttac ggcaggaatt cagaaagatc
     1861 aggtacggtc agtccaagat cgattcactg aatgtggaag aaatcattca aaggctggaa
     1921 actctgatga aggaagaaaa ggctttttca gatgaagatc tgtcccttcc gtcactgtcc
     1981 gaagagctcg gcatttccgt tcatcagctt tcacagatac tgaa
//
LOCUS       DAOQGJ010000680         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38159_length_2020_cov_3.489567, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000680 DAOQGJ010000000
VERSION     DAOQGJ010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38159_length_2020_cov_3.489567"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PK683_10955"
     CDS             <1..384
                     /locus_tag="PK683_10955"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNH09012.1"
                     /translation="AGLSVSSGGTVRPDYRESRFSDNSATDILNNILGFETFYTSGAE
                     SSFSMLTAYYNPELNGKIIKKISEVKSSLSLLNTFKSDLNSGNRTNIQTVMTKVGELR
                     ALIKTDLSNALGSSGSSSGSGGDGD"
     gene            377..>2020
                     /locus_tag="PK683_10960"
     CDS             377..>2020
                     /locus_tag="PK683_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH09013.1"
                     /translation="MIRFFHFIIILYASAVYGQETVPKKAPPTAEEIEAEKKRMEKMY
                     EKAAINVIGDNKDSLKKIPGSATLVEKKFLEETQPLDAMEVLRRVPGASTRFMDSAGL
                     TPNIGFRGVSNEESRKTLILEDGVLTSLSPYGQPESYYSPQIDRMSRVEIVKGSGSIL
                     FGPSTIGGIVNFVTRKPPAKNTFSTRTMGGENGYFSSLNQFGGTTGKTGYDISYLHKQ
                     GDGYRDYNHFNVNEANLKLVHALNDRHTFTFKLSVHEQKARATYLGLTEGLYWKNQRI
                     NPAQFDLKELNRTSAVLGHEYTLNENNRIITKVYATGAQRNWQREDYAYNNYSQYGTA
                     SIPPTDTYQIYAPAPVGNRPGDVLYMRTGAPMRNQGFGTLGIETKLESSFSLGFTKHE
                     VDLGARAHGEQNKINFKQQPSFLQYPFIREGIPFSQQDRVIRAYALYFQDRISLTDKF
                     KVIPGLRYENISQGVYTKRRKATARDVREKRAGAEGDIIFVDQGGETYVKILLPGFGV
                     TYDISKDFIWFAGVHKSFSPPTFGTSISPYGQDYRLGAET"
BASE COUNT          634 a          408 c          498 g          480 t
ORIGIN      
        1 gccggacttt ctgtctcttc cgggggaact gtcagaccgg attacagaga gtcccgtttc
       61 tctgacaatt ctgcgacgga catactgaac aatatcctgg gttttgaaac attttacaca
      121 tcaggggctg agagcagttt ttcaatgctg acagcgtatt acaatcctga gcttaatgga
      181 aaaataatta aaaaaatttc agaagtgaaa agttccctga gtcttttgaa tacgttcaag
      241 tctgatctga attcaggaaa cagaacaaac atacagactg taatgactaa agtcggggaa
      301 ctcagagccc tgataaagac tgatctgtcg aatgcgctcg gttcttccgg aagttcgtca
      361 ggatccggag gcgacggtga ttagattttt tcactttatc attatattat atgcatcggc
      421 agtctatgga caggaaacgg ttcccaaaaa agcccctcct actgctgaag aaattgaagc
      481 agaaaaaaaa agaatggaga aaatgtatga gaaggcggct atcaatgtca taggcgacaa
      541 taaggacagt ctgaaaaaaa ttcctggatc agccactctt gtggagaaga agtttctcga
      601 agaaactcag cctcttgacg cgatggaggt tctgagaagg gtgccgggag catctacccg
      661 ctttatggat tctgcaggac tgactccgaa tatcggattt cggggagtga gcaatgagga
      721 gtcgagaaag acactgattc ttgaagacgg agttctgact tctctgagtc cttacggtca
      781 gccggaaagc tattactcgc ctcagataga caggatgtca agggtggaga tagtgaaagg
      841 ttcaggatcc atactgttcg gtccttctac aataggggga attgtaaatt ttgtcacaag
      901 aaaaccgcct gcaaagaata cattcagcac aagaacaatg ggcggcgaaa acggatactt
      961 ttcttcactg aatcagttcg gaggaaccac aggaaaaaca ggatatgata tatcctatct
     1021 tcacaagcag ggtgacgggt acagggacta taatcatttt aatgtgaatg aagccaacct
     1081 gaagcttgtc catgctctga atgacagaca tacatttact ttcaaactca gcgtccatga
     1141 acagaaagcc agagctacct atctcggact gactgaggga ctgtactgga agaatcagag
     1201 gatcaatccg gcacagtttg atctgaagga actgaacagg acctctgcgg ttctaggcca
     1261 tgaatatacg ctgaatgaaa acaacagaat catcacaaag gtatatgcaa ccggcgctca
     1321 gagaaactgg cagagagagg attacgctta caataattac agtcagtacg gaacggcttc
     1381 aattccgccg acagacacat atcagattta cgcaccggct cctgtcggaa acagacctgg
     1441 agatgttctt tatatgagaa ccggagctcc gatgagaaat caagggtttg ggaccttggg
     1501 aatagagaca aaactggaaa gcagtttcag tctggggttt actaaacatg aagtcgatct
     1561 gggggcaaga gcacacggag agcagaacaa gatcaacttc aaacagcagc cttcattcct
     1621 gcagtaccct ttcatcaggg aaggaattcc tttcagtcag caggacagag tcattcgtgc
     1681 atatgcactc tattttcagg acagaatcag tctgaccgat aaattcaaag tgattcctgg
     1741 gcttcggtat gaaaatatat ctcagggagt atataccaag agaagaaagg caacggcaag
     1801 agacgtgaga gaaaaaaggg caggtgcgga aggggatatt atttttgtag atcagggagg
     1861 ggagacctac gtgaaaattc ttcttccggg attcggggtg acatatgata tatcgaaaga
     1921 ttttatctgg tttgccggag ttcataaaag cttctctccg cctacattcg gaacttccat
     1981 cagtccctat ggacaggatt acagactggg agccgagaca
//
LOCUS       DAOQGJ010000681         2019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38214_length_2019_cov_1.865580, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000681 DAOQGJ010000000
VERSION     DAOQGJ010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38214_length_2019_cov_1.865580"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK683_10965"
     CDS             <1..350
                     /locus_tag="PK683_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09014.1"
                     /translation="ESGAAYHTNFADETGLRNGEFGMHGTDDIVLLFGSDSSFPESYG
                     GSRERAELRGKNYESAFSHLPAYTRLGFAAALLGGVRFEFNIGELADFLLGFFLIDIY
                     GDDVYLNTRFVQN"
     gene            676..1089
                     /locus_tag="PK683_10970"
     CDS             676..1089
                     /locus_tag="PK683_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09015.1"
                     /translation="MEFIQKNNLFSSVQMGYSPGYRLQYTENFKMPYYDRLGGFPNDG
                     NYYEYQYNRHPSPLIGIGAGKKIFSPEGFYFSFEIGGRYFLNSSTSVYAKPADFNIFL
                     NAAPPSPIAVYLAERKIRRRSSHYEIYFGFQIGVF"
     gene            complement(1101..>2019)
                     /locus_tag="PK683_10975"
     CDS             complement(1101..>2019)
                     /locus_tag="PK683_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09016.1"
                     /translation="NFFLILSANQNIFRKGQYYMQAYCMTMKESWKIFTVLLLFLHIM
                     CFGGGVKKTAFEERKEKAFETLQSGKEQLLGKNWEKSIDKFDRACREFALIDEKEGLL
                     KCRTEKARYFIRKENYREAESWLAKAREDSISLSKIYLRSVMNLFIFLEMKRKNFEGT
                     EKYIAEAEKLSGEKEEMMDTWYISGMTFFEKKDWSRAAEYLEKSSGSERERTVSSSYA
                     FLSEIRLREKKYESAMNYSEKAASIDRKNAWPSFLAYDYVLSSESCAGLGKKEEAVFF
                     RKKALEIHDALERKESSEKDRKFISEIMK"
BASE COUNT          552 a          407 c          396 g          664 t
ORIGIN      
        1 tggagtccgg tgcagcgtat cacacgaatt ttgcagatga gaccggactt cgaaacgggg
       61 aattcggaat gcacggcaca gatgacattg tgcttctgtt cggatcggat tccagttttc
      121 cggaaagtta cggcggaagc agggagaggg ctgaactgcg cggaaagaac tatgaatcag
      181 cattttccca tctgcctgca tataccaggc tcggttttgc agctgcactt ctcggaggtg
      241 tccggttcga gttcaatata ggagaacttg ctgatttcct gcttggtttt ttcctgattg
      301 atatttacgg ggatgatgtg tatctgaaca cccggttcgt acagaattga actgaggcat
      361 acgagaaaag cagatgataa aacaaattat atttattttc tcagtattca tatctttctg
      421 ctctgctgtt ttcgctcagt cttctgaaaa acctcagagt gaagaaccta aaaaagagtt
      481 tgcagttttt acttcagtgt acaataataa tcagggactt gggactcttt acaggttcag
      541 aaagaacact gcggtatctt ttcagttctt ttttaatacg gatcagcaga ataaaacagt
      601 tctgaatcag gggccttgcg atatggtttt atgcagttat gaaaaaagaa catatggctt
      661 aaaagtattc ttgcattgga attcattcag aaaaacaatc ttttttcatc tgttcagatg
      721 ggatacagtc ccggatacag gcttcagtat actgaaaact ttaagatgcc atactatgat
      781 cgtctgggtg gattccctaa tgacggaaat tactatgaat atcagtataa cagacatcca
      841 agtcctttga ttggaattgg agcaggaaag aaaattttca gtcctgaggg attttatttc
      901 agtttcgaaa taggaggcag atatttttta aacagcagca cttccgttta tgcaaaacca
      961 gccgatttta atattttcct gaatgcggca cctccctctc cgatcgcagt ataccttgca
     1021 gaaagaaaaa tccggcgtag aagcagtcat tatgaaattt atttcggatt tcaaataggt
     1081 gttttctgag ctgtaaaaaa ttacttcata atctcactta tgaattttct gtctttttct
     1141 gaagattcct ttctttcgag agcatcatga atttctaatg ctttttttcg aaaaaatact
     1201 gcttcttcct ttttgccgag acctgcacag ctttcggagg agagcacata gtcgtatgca
     1261 agaaaggaag gccaggcatt tttcctgtca attgaggcag ccttttctga atagttcatc
     1321 gcagactcat attttttttc gcggagtctg atctcagaaa gaaaagcata ggaactcgaa
     1381 actgttcttt ctctttcact tccggaagat ttttctaaat actctgcagc gcgtgaccag
     1441 tctttttttt cgaaaaatgt catccctgaa atataccagg tatccatcat ctcttctttc
     1501 tcaccggaaa gtttttccgc ttcagcaatg tatttttctg tgccttcaaa attttttctc
     1561 ttcatttcca ggaaaataaa aagattcatt actgatctca ggtagatttt actgagggag
     1621 atactgtcct cgcgggcttt tgcaagccag ctttctgctt cacggtagtt ttcctttctt
     1681 atgaagtacc ttgccttttc cgtcctgcat ttcagaagtc cttctttttc atcaatcagt
     1741 gcaaattccc tgcaggccct gtcgaattta tcgatggatt tttcccagtt ttttccgaga
     1801 agctgctctt ttccggactg aagagtttca aaggcttttt ccttccgttc ctcaaatgcc
     1861 gtttttttga ctcctcctcc aaagcacatg atatgcagaa acagcagcag tacagtgaat
     1921 attttccaag attctttcat tgtcatacag tatgcctgca tataatattg accttttctg
     1981 aaaatattct gattggcaga taaaatcaga aaaaaatta
//
LOCUS       DAOQGJ010000682         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38236_length_2018_cov_2.790117, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000682 DAOQGJ010000000
VERSION     DAOQGJ010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38236_length_2018_cov_2.790117"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1381
                     /locus_tag="PK683_10980"
     CDS             305..1381
                     /locus_tag="PK683_10980"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HNH09017.1"
                     /translation="MNKKTVFLFCAAAASSVIMAISVKKDETIIFFPTVSKLNSETGK
                     WDVLIHGQVFEKEEDSKTRNYFIEFLRSRMKVEQNTPEADIFKERMRWFLIDNQRWKT
                     VKVRLQNLSYKLNRSKANGHFEDQIKLEVKKESDGKFPETVSYTAELPDDDSRHFSGK
                     TYLVPDTGISLVTDIDDTIKISDVRNKDNLLKNTFQKPFKEVPGMGDLYSSWAKKNVQ
                     INYVSASPWQMYTMLSEFFQSKGFPDGVYHMKYVRLTDTDFFNLMLAPDKYKISTIEP
                     LMKLFPKRKFILVGDSGEKDPEAYAFLAEKYTEQVLQILIRDAYPEDGPKRLNEVFSK
                     IPKEKWQSFQNASDIKKDFLKNIL"
     gene            1416..1814
                     /locus_tag="PK683_10985"
     CDS             1416..1814
                     /locus_tag="PK683_10985"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HNH09018.1"
                     /translation="MKTSEGNRLIYISGAALCLLLMICILLFFPKTGWIRGTAGDFIV
                     VVFLYFSLRGSFLLSPIRSALISLAVSFGIEGLQYLRILDLLHLSDFKAARIIFGSVY
                     DPMDLIAYAAGAGAAFFSDTGFLSFCSPGQ"
BASE COUNT          626 a          419 c          427 g          546 t
ORIGIN      
        1 aatgatgcaa tctacagaat gcttattcct attccggaca gaaagaccgg tcatgaactg
       61 aacactaaac cgagcactga actcactaaa tatacaggct tgcggtttga ctggccttcc
      121 gataaaaaag atcccaacgt ttaccttttt caaggaactg atctgtaaat atctttcata
      181 aaaattcaga ttccaggaaa aaaattactg tacagacttg gaatctgctg aatactgtta
      241 taaaaacatg agttcagtgt cctgacgaat tcacgttcag aaaatataca aggcatatta
      301 ttttatgaat aaaaaaacgg ttttcctttt ctgtgccgcc gccgcaagtt cagtcattat
      361 ggcaatttca gtgaaaaaag atgaaacgat aatattcttc cctactgttt cgaagctgaa
      421 ttctgagact ggaaaatggg atgtgcttat tcacggtcag gtttttgaaa aagaagaaga
      481 ttcaaagaca agaaactact tcatcgaatt tctgcgctcc agaatgaagg tagaacagaa
      541 cactccggaa gccgatattt tcaaggaaag aatgcgctgg tttctgattg acaatcagag
      601 atggaagact gtcaaagtca ggctccagaa tctttcctat aagctgaaca gatccaaggc
      661 gaacggacat tttgaggatc agatcaaact ggaagtaaaa aaggaatctg acggaaagtt
      721 tccagaaaca gtctcataca ctgcagagct tcctgacgac gattccagac atttcagcgg
      781 aaagacctat ctggttccgg atacagggat ctctcttgtg acagacatag atgatacgat
      841 taaaatcagc gatgtcagaa acaaggacaa ccttctgaaa aatacatttc agaaaccttt
      901 caaggaagtc ccgggaatgg gagatctcta ttcatcctgg gcaaaaaaaa atgtacagat
      961 taattatgta agtgcaagcc cctggcagat gtacacaatg ctctccgaat tctttcagtc
     1021 caaaggattc cctgacgggg tttatcatat gaagtacgtc aggcttacgg acaccgattt
     1081 tttcaatctg atgttggcac cggataaata caagatcagt acaatagaac ctctgatgaa
     1141 actgttcccg aaaaggaaat tcattctcgt cggagattca ggagaaaaag atccggaggc
     1201 ttacgccttt cttgcagaaa aatatactga gcaggtgctt cagattctga tcagagatgc
     1261 atatccggag gacggcccga agagactcaa tgaagttttt tcaaaaattc cgaaggaaaa
     1321 atggcagtca ttccagaacg cttcggacat aaaaaaggac ttcctgaaaa atattctata
     1381 atgaaaaaag attatttttg ccgcaccatt catatatgaa aacttcagaa gggaacaggc
     1441 tgatttatat ttcaggcgca gcgctctgtc ttctgctgat gatatgcatt ctgcttttct
     1501 ttccaaaaac aggctggatc agaggcacag cgggagattt catcgttgtg gttttccttt
     1561 atttttcctt gagagggtct tttctgctga gtccaatccg ttccgcactg atttctctgg
     1621 cagtgtcgtt cggcatagaa ggtctgcagt atctgaggat actggatctt ctgcatctct
     1681 ccgacttcaa ggctgccaga atcatcttcg gctcagtcta tgatcctatg gatctgatcg
     1741 cttatgctgc aggtgcaggc gccgcatttt tttcggacac cggatttctg tccttctgca
     1801 gtcccggaca ataaatctga tcccgaaact gctcctgctg aaaaaacttt tcattcagaa
     1861 ccagggaagt gaccggttct gggccgggtt tatgtaatga gtctataatt aaacagtact
     1921 tcagaaacag ttttttcggc agaagatgca gatgcatcat tgcaccgaat gacgcacttg
     1981 gactgctcaa taaagtttcg acaccgcata cgaagggc
//
LOCUS       DAOQGJ010000683         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38249_length_2018_cov_2.188487, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000683 DAOQGJ010000000
VERSION     DAOQGJ010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38249_length_2018_cov_2.188487"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK683_10990"
     CDS             complement(<1..206)
                     /locus_tag="PK683_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09019.1"
                     /translation="MSAELGLMEKMICDNCIGSLIPVKGNKNVFICEYCKSKYYMQNP
                     GDPMQKMERFTVNKVKVFIIAAAVS"
     gene            276..>2018
                     /locus_tag="PK683_10995"
     CDS             276..>2018
                     /locus_tag="PK683_10995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH09020.1"
                     /translation="MSQINMNLNSFFLHLTLRLELFTHTVPLPFAIYLGAVASGLDAE
                     NQIIMLVTCFSWAGMTVVAGVIWRYFRIRSFFKTFSQLEKAQTKNTAEIQRLKLRLMR
                     YPFWEGAVIVIRWIFGATPAVIHTGFIIGFDKAALVLQLLPFVILMITPLSFMAYFFI
                     TEKSLSILMERPCLAQAEIPPEKLTKFNYYIRIILTVFSLANMPFTLFGYMLYALHKG
                     YLTVDNPVLHVLILAVMFSLPIIFVAYTLGNSVRRGISAVSSSLQNVGMGDFSVKLPV
                     AAMDDFSVQAYHLNQVIARLSSMYSEIRDLNSNLEEKVRQRTDELNRILYEQRGDYFL
                     TSLLLEPLGRLEVESRRFTVEFFTRQKKNFEFKEKNLEIGGDLNISKTLTLQGRTYHM
                     FVNSDAMGKSLQGAGGALVLGAVLKSILDKTEHSALMSSLGPSDWLRDTFYDTHKVFE
                     SFDGSMLVSFIIGLVEEETGTTHFINADHPWMILFRDGKASFVECFIHRKLGFPEWLD
                     NVQVGMVELAPGDILISGSDGRDDIELPEMINGERVINEDEFQFLRRIEEAGADLQKI
                     YEGISGFGKIIDDFS"
BASE COUNT          502 a          407 c          481 g          628 t
ORIGIN      
        1 gagactgcgg ctgcgatgat gaaaactttc actttattga cggtgaacct ttccatcttc
       61 tgcataggat cgccgggatt ctgcatatag tatttcgatt tgcagtattc acagatgaat
      121 acattcttgt ttccttttac aggtatcagg ctgccgatgc agttgtcgca gatcatcttt
      181 tccatgagtc ctaattcggc ggacaatgga tttttcgtca agtcccttgt tttctgattt
      241 gtacttgttt ttttggcaca gttctctttc attgtttgag tcaaatcaat atgaatctga
      301 attcattttt tctgcacctt acactcaggc tggagctttt tacacatact gtacctctgc
      361 cctttgcgat ttatctggga gcagttgcat ccggtctgga tgcagaaaat cagattatta
      421 tgcttgtcac ctgtttttcc tgggcaggga tgactgtcgt tgccggagtc atctggcggt
      481 acttccgcat cagaagtttt tttaagacat tcagccagct ggaaaaggct cagacaaaga
      541 atactgcgga gattcagaga cttaaactca gactgatgcg ttatcctttc tgggaaggtg
      601 cagtcattgt cattcgatgg attttcggtg ctacgcctgc ggtcattcat acaggtttca
      661 ttatcgggtt tgacaaagcc gctcttgttc ttcagcttct tcctttcgtg attctgatga
      721 taacgcctct gtcctttatg gcatattttt tcattacaga aaagtcgctg tccattctta
      781 tggagaggcc ctgccttgcg caggcagaaa ttccgcctga aaaacttact aagtttaatt
      841 attatataag aattatactg actgtcttca gtctcgcaaa tatgccgttt acactgttcg
      901 gatatatgct gtatgctctg cacaaaggct accttacagt ggacaatccg gtactgcatg
      961 tgctgattct tgcagtgatg ttttctcttc cgattatctt tgttgcctat actctcggaa
     1021 attctgtcag acggggaatc tctgcagtaa gcagttctct tcagaatgtg ggaatgggag
     1081 acttcagtgt caagcttccg gtagcagcaa tggatgattt ttctgttcag gcatatcatc
     1141 tgaaccaggt gattgccagg ctgagttcca tgtattcgga gattcgcgat cttaattcaa
     1201 atctcgagga gaaggtgagg cagagaacgg acgaactgaa taggattctg tatgaacaga
     1261 gaggggatta ttttctgaca tctcttcttc ttgaacctct gggcagactt gaagtggaaa
     1321 gcagaagatt cactgtggag ttttttacaa ggcagaagaa gaattttgag ttcaaggaaa
     1381 aaaatctgga aattggcggg gatctgaata tatcgaagac tcttactctt cagggaagga
     1441 catatcatat gtttgtcaat tcagatgcaa tgggaaaatc tctgcagggg gccggaggtg
     1501 cccttgtgct tggggccgtc ctgaaatcca tcctcgacaa gactgagcat tctgcactga
     1561 tgagcagtct cgggccttca gattggctca gagacacttt ctatgacaca cacaaggtat
     1621 tcgagagttt cgacgggtct atgctggttt catttatcat cggccttgtg gaggaagaaa
     1681 ctggaacgac tcatttcatc aatgcagatc atccctggat gattcttttt cgggacggca
     1741 aggcatcctt tgtggaatgt ttcattcaca ggaaactcgg tttccctgaa tggctggaca
     1801 atgtacaggt gggaatggtg gaacttgcgc cgggagatat tctcatcagc ggttctgacg
     1861 gaagagatga tattgaactg cctgaaatga ttaacggtga aagagtcatc aatgaagacg
     1921 agtttcagtt cctgagaaga attgaagagg ccggagctga tctgcagaaa atttatgaag
     1981 gcatttccgg tttcggaaaa atcatcgacg atttctct
//
LOCUS       DAOQGJ010000684         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38264_length_2017_cov_3.268094, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000684 DAOQGJ010000000
VERSION     DAOQGJ010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38264_length_2017_cov_3.268094"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PK683_11000"
     CDS             <1..1054
                     /locus_tag="PK683_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH09021.1"
                     /translation="IVNYSAASANTANINTLAQDINNLPNRVRINGFGAFYQHTYKPS
                     DYWIFSAGYRVDYNSIFGRSQTPRISGIYKPTQNLTLKLLLGTGFREPSSFEFLNRTS
                     TRKENLNLRPEYLRSVEFGIAYRWNKLYLSAQSYYNRVTNLISTVRTLEPIPGNTSTW
                     QQNQNVGKAEIFGTEIEANLSITEKFSLNANYTYSKGRYLDLSRSLTSSPSSFGRPGD
                     DIFSDILNAYKIQNIPSSGPIPHIAPHKFYLGAVYSFWKKISIYAGINYVDIRRTIVT
                     NPVKTAPGYIMGKLNFRWEDFIKEGAYLNILAVNLSNDLFFDPGVIGGTGGLGSAPTL
                     HPLEKRNIWISLGYNF"
     gene            1054..>2017
                     /locus_tag="PK683_11005"
     CDS             1054..>2017
                     /locus_tag="PK683_11005"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNH09022.1"
                     /translation="MLTFRSFQKIIYFFTLSFIYCSPLIADDNRDISVPIIVSSSNPI
                     YLQALAGFQYSFRHKNSIYYMNSVREEYPDLKDFYSQFRSEEIPLIITIGNDAGRTAR
                     EYIKDKPTLFLMIDAPKLMKPEQGKFCGYEKDVSFSEYFSTLRELKPSARRVLTVYST
                     DEGEILTGAGEYEDIWKGVLFRRFKAASKDELKRILNDNLGKFDAFYMSSDRLYDQES
                     FEYVSDFCRKNGIILMTLYPALVELGATFALVPDFSNQGVQAGQMANQITEGKTECEM
                     GPVLQPEQPFLYLNENYSQESGVTIPDSLKIKLKTDRILSFGIDL"
BASE COUNT          636 a          419 c          406 g          556 t
ORIGIN      
        1 aatagtcaac tattctgccg cttctgcaaa tacagccaat ataaacactc ttgctcagga
       61 tataaacaat ctcccaaaca gagtcagaat caacggattc ggcgcatttt atcagcacac
      121 atataaacct tccgactact ggattttttc ggcaggatac agagtggatt ataattcaat
      181 attcggcaga tcacagactc ctcgcatcag cgggatttac aaacccaccc aaaacctgac
      241 tttgaagctc cttttaggga caggattccg tgagcctagc agttttgaat ttctgaacag
      301 aacgtccaca cgcaaggaaa atctgaatct cagaccggaa tatctgcgct ctgtcgaatt
      361 cggaatagcc tacagatgga ataaactgta tttgtctgca cagagttact acaacagggt
      421 cacaaatctg atttccaccg tcagaacact ggaaccgatt ccggggaata caagcacctg
      481 gcagcagaac cagaacgtgg gaaaggcaga aatattcgga acagaaattg aagccaatct
      541 cagcattacc gaaaaatttt ccctgaatgc aaattacact tacagcaaag gccgatatct
      601 tgatctttca agatcactga catcctcacc ttcttcattc ggaagacctg gagacgacat
      661 attttcagat attttgaatg catataaaat acagaatata ccttcatcag gaccgattcc
      721 tcatattgct ccacataagt tttatcttgg tgctgtatac agtttttgga aaaaaatcag
      781 catttatgcg ggaatcaatt acgtcgacat caggagaaca atagtcacga atcctgtcaa
      841 aacagctcct ggttacataa tgggaaaact taattttcga tgggaggatt ttattaaaga
      901 gggagcttat ctgaatattc tcgcggtaaa cctgtccaat gatctttttt ttgatccagg
      961 agtcattggg ggcacaggtg gccttggatc cgcaccgacc ctgcatcctc ttgaaaagag
     1021 aaacatctgg atatctttgg ggtacaattt ttaatgctga ctttcagatc ttttcaaaaa
     1081 attatatatt tttttacatt atcctttata tattgcagtc ctttaattgc ggatgataac
     1141 cgggacattt ccgttccgat cattgtatcc tccagcaatc cgatctatct tcaggcactt
     1201 gcgggctttc agtattcctt caggcataaa aactcaattt actatatgaa ttctgtcaga
     1261 gaggagtatc ctgacttgaa ggatttttat tcacagttca gatccgaaga aattccgctc
     1321 atcatcacta tcggaaatga tgcaggaaga actgcaagag aatacatcaa ggataagcct
     1381 actctgtttc tgatgattga tgcacctaaa ctgatgaagc ctgaacaggg aaaattctgc
     1441 ggctatgaaa aagatgtttc attctctgaa tacttcagca ctttaagaga attgaagcct
     1501 tctgcaagaa gagttctgac agtatacagc actgatgaag gtgaaattct gacaggcgcg
     1561 ggagaatatg aggatatctg gaaaggggtt ctgttcagaa gattcaaggc tgcatcaaag
     1621 gatgaactga aaagaatact caacgacaat ctgggaaagt ttgatgcatt ttatatgtca
     1681 tccgacagac tttacgatca ggaaagtttt gaatatgtct ccgatttctg cagaaagaac
     1741 ggaataattt taatgacttt atatcctgca ctggttgaac ttggagcgac atttgcactc
     1801 gttccggact tttcaaatca gggagtccag gcgggtcaga tggcaaatca gattacagaa
     1861 ggaaagactg aatgcgaaat gggtccggtt cttcaaccgg aacagccgtt cctgtatctg
     1921 aatgaaaact attcacagga atcaggagtg acaatacccg actctctgaa aatcaagctg
     1981 aagacagaca gaatcctttc ctttggaata gatctat
//
LOCUS       DAOQGJ010000685         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38273_length_2017_cov_2.747706, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000685 DAOQGJ010000000
VERSION     DAOQGJ010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38273_length_2017_cov_2.747706"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK683_11010"
     CDS             <1..198
                     /locus_tag="PK683_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09023.1"
                     /translation="KRLVDDWTELGRNYVTAFKALSEEQLDGLNMPHPILGNITVREM
                     ILFSLMHVFHHVKKYIEKTAR"
     gene            200..499
                     /locus_tag="PK683_11015"
     CDS             200..499
                     /locus_tag="PK683_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNH09024.1"
                     /translation="MSSVDEFLKKHPSWQIREGKLISEFVFSEYRKAVSFFIRLSELA
                     ERKNHHPEVTVGFRKVTVILHTFDVNEITEKDIESAEETEKIYHTLRFPNSGKEM"
     gene            1242..>2017
                     /locus_tag="PK683_11020"
     CDS             1242..>2017
                     /locus_tag="PK683_11020"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HNH09025.1"
                     /translation="MEFPAGLDWINADKPVKMKDLKGRISILYFWTYSCTKCSDIPEE
                     LTEFSEKWKDVVTVIGIHSGKFPNEKDSANVRTAVRREGISFPVVNDPNLVLWRAYGL
                     NSWPAFILIGADGKLIGRNTGRNFREQFDSIFEDIVRESEKKGELKRNAVSLPVSASA
                     KTEPALLFPSSLHLAKDHKTLYISDTNHHRVIIFDLEKNEIQSVIGTGRRGNRNGKFS
                     EAEFSHPSGLVLKDDMLYIADRKNRSIRRADLKKKEVSVIL"
BASE COUNT          639 a          378 c          430 g          570 t
ORIGIN      
        1 aaaagacttg tcgatgactg gacggagctc ggcagaaact atgtgacagc tttcaaggct
       61 ctctctgagg aacagctgga cggtctgaat atgcctcatc cgattctggg taatattact
      121 gtcagagaaa tgattctctt ttcactgatg cacgtatttc atcatgtgaa gaaatacatc
      181 gagaaaacgg ctcgttgatt tgagcagtgt tgacgaattt ctgaaaaaac atccttcatg
      241 gcagatcaga gaaggaaaac tgatttcgga atttgtattt tcggaatacc gcaaggcagt
      301 ctcttttttc ataagactgt ccgaacttgc ggagagaaaa aatcatcatc ccgaagtgac
      361 tgtcggtttt cgcaaggtaa ctgtaattct gcatactttt gacgtgaatg aaattacaga
      421 aaaggatatt gaatctgccg aggaaactga gaagatatat catacattac gattcccgaa
      481 ttccgggaaa gagatgtaag gcaaaagata atgatatttt catgcaatta ttcactgatt
      541 gtctgaaatt ctgaatgcaa tgacggttga ctttccgttc aggatatttt ttcctactga
      601 agctcttctt caatttcgtt taccggcata tggattaaac aggtcttcat aaatgattgt
      661 actttttccg gttggcagac ttgattttac tgagccgtga ataagtagac aggcaaagac
      721 ttttgaaaac tattactcaa atttctaatg aaatttcata aagtcaattc atcattgact
      781 gcattaaatt tgcagatttc cttcagttca taactcacct tacgcaaaaa gaatacaatg
      841 aatagaacct ataattttta cgaaacacta atccggcagt ttaaaagcct gcgatgaaac
      901 aaaaagaact tgagtttagc cagcttctga atattggcgg cctcctgcag gcgttcctcc
      961 cttatacggt gttccttatc cggcagggag ttctgtctgt actttgaaaa atgggcctat
     1021 gtcagccatt ccggactgaa atgttttcac attttctttt tcagaattga atgaactaat
     1081 tcttgacaga aataaaaaag tctgatccaa ttataaaaat gcgattcaga tttttttttc
     1141 agatttttct ttttttcagt ccagttctta taacgctcag ctgcagagac agggaccgga
     1201 tagtcgagct ggaaaaagaa agagaaaatg aaatcggata tatggagttt cctgcagggc
     1261 tcgactggat caatgcagac aaacctgtaa agatgaagga tttgaaagga agaatttcca
     1321 tactgtattt ctggacatac agctgcacga aatgcagtga tattccggaa gaactgacag
     1381 aattctcgga aaaatggaag gatgtggtca ctgtcatcgg aattcattca ggaaaatttc
     1441 cgaatgaaaa agattcggca aatgtcagga cagcagtcag aagagaaggg atcagtttcc
     1501 cagtagtcaa tgatcctaat ctggtgcttt ggagagctta tggactgaac tcctggcctg
     1561 cattcatact gatcggtgcc gacggaaaac tcatcggcag aaacaccggc cgaaacttca
     1621 gagaacagtt tgacagtatt tttgaggata ttgtcagaga atccgaaaaa aaaggggaac
     1681 ttaagagaaa tgctgtctcg cttcccgtat cggcttctgc aaagaccgag cccgcacttc
     1741 tttttccgtc cagcctccat ctggcgaagg atcataagac tctttatatt tcagatacaa
     1801 atcatcatcg agtaattata ttcgatctgg aaaaaaatga aatacagtct gtcattggaa
     1861 caggccgaag agggaacagg aacggcaagt tttcagaggc ggagttcagt catccttccg
     1921 gtcttgtact gaaggatgat atgctttata tagcggacag aaaaaacaga tccataagaa
     1981 gagcagacct gaagaaaaaa gaggtctctg tcattct
//
LOCUS       DAOQGJ010000686         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38290_length_2016_cov_3.639470, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000686 DAOQGJ010000000
VERSION     DAOQGJ010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38290_length_2016_cov_3.639470"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK683_11025"
     CDS             <1..543
                     /locus_tag="PK683_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09026.1"
                     /translation="FYRDEKSERISARAVTEKTVFEVWSASSEHPVLSGPMLIPFYSV
                     EPPVLNSEQLKLVFEFSSLKEKTNFNGKIMKESLKLRLNDSEDVYPDLAVLYEKNKDG
                     IYEIRKSYSGTPEYIPFNGDAYVEMTFFSAVYSELKQYSFQPALRLKSSDAECPLLKF
                     RPERKRTYIPFRFPFSLPMK"
     gene            complement(546..>2016)
                     /locus_tag="PK683_11030"
     CDS             complement(546..>2016)
                     /locus_tag="PK683_11030"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09027.1"
                     /translation="NNIIIKVPRKIYFIPKEIGLLKNLTELNLNSCGIKELPAEIWNL
                     TNLEILSLKANELVFLPEGKVNLKKLRTLDLSENRLQTLPDEIGNLNSAEQLRLSGNQ
                     LETVPASLGNMKNLKILVLDDNRIRKIPKELGNLNLLETMNISRNQLDEIPSEISGMS
                     SLKILRMNQNKIKSIPKGFKLLKKLVSIELSDNSISAFSAELSDLPELSHLVFSGNRI
                     SSLPDETGNMKSLRSISLSGNQFKAFPEELTILSDLDSLSLGQNQIPSVPAKIERMKN
                     IRFLDISDNQIKFIPVEVCSLKNLTNLRAEGNLLKTVPKEIGKLSKLTELAIFRANPL
                     ESIPKEFYKLNIFSEFDWVRNGWDYDMSDTPWDSRIIDGAYKLIEKCRTQKSISLDLN
                     EKRLFAVPVEVFELKHLKELDLSGNILKFIGTAMEKLQNLEHLRMNGLKKSLTRLDCD
                     FTTFKKLRTLELKETELPKGVEAKLKIWASKNGCLITFS"
BASE COUNT          543 a          393 c          413 g          667 t
ORIGIN      
        1 ttttacagag atgaaaaatc tgaaaggata tcagcgagag cggtcaccga aaaaacggtt
       61 tttgaagtat ggtcggcttc ctctgaacat ccggttctgt ccgggcccat gcttattcca
      121 ttctattcag ttgaacctcc tgtactgaac agcgaacagc tgaagcttgt ttttgagttt
      181 tcttctctga aagaaaaaac aaattttaat ggtaaaataa tgaaagaaag cctgaaactc
      241 cgtctgaacg acagtgagga tgtgtatcct gatctggcag tgctgtatga gaaaaacaaa
      301 gacggtattt atgaaatacg caaatcatat tcaggtactc ccgaatacat accttttaac
      361 ggtgatgcat atgtcgaaat gacttttttt tcggcggtgt actccgagct gaagcagtat
      421 tcttttcagc ctgcactcag gctgaaatca tcggatgctg aatgtcctct gctgaaattt
      481 cggcctgaaa ggaaaagaac ctatattcct ttccgttttc ctttttctct tccaatgaaa
      541 tagattcagg aaaaagtaat cagacatccg ttttttgatg cccatatctt aagttttgcc
      601 tccacccctt tgggaagttc tgtttctttc agttccagcg tcctcagttt cttgaaagtt
      661 gtaaaatcac agtcgagacg ggtcagggat tttttcagtc cgttcattct gagatgttcc
      721 agattctgca gtttttccat tgctgtcccg ataaatttca gaatgtttcc tgaaaggtca
      781 agttccttca gatgcttaag ctcaaatact tcaaccggaa cagcaaaaag tcttttttca
      841 ttaagatcta atgatataga cttctgtgtt ctgcattttt cgataagctt gtaggcaccg
      901 tcaataatgc ggctgtccca gggagtatca gacatatcat aatcccatcc gtttctgacc
      961 cagtcaaatt cagaaaatat gttcagttta taaaattctt taggaattga ttccagcgga
     1021 tttgctctga atattgcaag ctctgtcagt tttgaaagct tgcctatttc cttcggaaca
     1081 gttttcagaa ggtttccttc tgcacgcaga tttgtgagat ttttcagaga gcagacttct
     1141 acaggaatga atttaatctg attgtcgctg atatccagga atctgatatt tttcatcctt
     1201 tcaattttgg caggaacaga ggggatctga ttctgtccca gactgaggga atccagatcg
     1261 gaaaggatgg tcagctcctc aggaaatgcc ttgaattgat tacctgacag cgaaatagaa
     1321 cgtaatgatt tcatattacc agtttcgtca ggaagagagc tgattctgtt tccgctgaaa
     1381 acaaggtggg acagctccgg caagtccgag agttcagcag aaaaggcgga aatactgttg
     1441 tctgaaagtt caatactgac aagttttttg agaagtttga atccttttgg gatacttttt
     1501 atcttatttt gattcattcg cagaattttc aggctgctca tcccggaaat ttcagaaggt
     1561 atttcatcaa gctggtttct ggaaatattc atcgtttcca gcagattcag attccctagt
     1621 tctttcggga tttttctgat cctgttgtca tctagaacaa gtattttcag atttttcata
     1681 tttcccagag aagccgggac agtttccagc tgattgcctg aaagtctgag ctgctcggca
     1741 gaattcagat ttccaatttc atcaggaaga gtctgaagtc tgttttccga gagatcgaga
     1801 gttcttaatt ttttcagatt gactttccct tcaggcagaa agacgagttc atttgccttc
     1861 aggctgagta tttccagatt tgtcagattc catatctctg cagggagttc tttgattccg
     1921 caggaattga gattgagttc tgtcaggttt ttcagaagcc cgatttcttt tggaataaaa
     1981 tatatctttc tgggtacttt tataattata ttattt
//
LOCUS       DAOQGJ010000687         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38319_length_2015_cov_3.577551, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000687 DAOQGJ010000000
VERSION     DAOQGJ010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38319_length_2015_cov_3.577551"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PK683_11035"
     CDS             complement(<1..302)
                     /locus_tag="PK683_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09028.1"
                     /translation="MSSAVVGGFVFLGICLILSSFLVMSVRLYQEKPAFIAKILKYLL
                     IAGIWVIFSVASIAIGLRINDRHEQTYTRGMQSVNDIWGGAISQTPPLLTSNSVQM"
     gene            complement(405..833)
                     /locus_tag="PK683_11040"
     CDS             complement(405..833)
                     /locus_tag="PK683_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09029.1"
                     /translation="MFKGIILSSGILILIFLYFCFFITPKAGGVQISLAETLIPTAVI
                     SPFVLIAGGTVGYFAEHHIFSEGRSPFMQIFIFSMCIYVLGAILIGMVMIFADGSHPG
                     GLAEKLADGLALGGTGALLFAVFFIPLIAVLSYLLSKWTD"
     gene            1339..>2015
                     /locus_tag="PK683_11045"
     CDS             1339..>2015
                     /locus_tag="PK683_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH09030.1"
                     /translation="MTVKSALSGIQKDSIGAGNMSAVKKNTVKFETHLKKGNDYFSDA
                     DYPKALREYAKAVELDSGSAEAYYRIGSAYFNLAEYPNAVQNYTECLKIDPKHIQAYI
                     ERAGAYANLGDLDSSIEDLKTVLKLDKNHSDARFALKQGEELRKMAAKLCSAVDKKID
                     KNSNDPFLYNEKGLILQKAGEHILAVREFRKALNLKKDYAEAAVNCGISLYWNQDWKL
                     CIDMMTSA"
BASE COUNT          649 a          422 c          426 g          518 t
ORIGIN      
        1 tccatctgaa cggagttgga ggtcagaaga ggcggtgtct gcgaaattgc tcctccccag
       61 atatcgttca ctgactgcat tccccttgta taagtctgtt cgtgcctgtc attgattctg
      121 agacctattg caatcgatgc gacactgaat atgacccaga tcccggcaat cagaagatac
      181 ttgagtattt ttgcaataaa agccggtttc tcctgataaa gccttacaga cattacaagg
      241 aatgaggaaa gaatcagaca gattcctaag aatacaaaac cgccgacaac agctgatgac
      301 atattgaccc ccgtaaaatt cggaatgcag atcatagttc acggtatttg ttttactgca
      361 atcagaaatt ttatcagcag gggaaaccgg atttaccagt cagatcagtc tgtccatttg
      421 gagagaagat aggaaagaac ggcgatcagg ggaataaaga agacagcgaa gagaagagct
      481 ccagttcccc caagtgcgag cccgtcggcg agtttttcag caagtccgcc gggatgcgat
      541 ccgtcggcaa aaatcataac cattcctatt aatatagcac caagaacata tatgcacata
      601 gaaaaaatga agatctgcat aaacgggctt cttccctcgg aaaagatatg atgctccgcg
      661 aaataaccga cagtgccgcc tgcaatcaga acgaaaggag aaatgacggc cgtcggaatc
      721 agtgtttcag caagactgat ctgaaccccg cctgcttttg gagtaataaa aaaacagaaa
      781 tataagaata tcaggatcag aattcctgaa gacaagatga ttcctttgaa catcgtatac
      841 cctctcttaa aaataaatca gaccattacc tcaaaaatct ttttgccttc cacttctttc
      901 cagctgtcgg tgaatttttt gtttttatta aaattgaata ccgctaaata tcctttctga
      961 tgttctttac atcatgcacc cctgccagga ttacgctgtg aaaagcctgg tccttgcccg
     1021 cctctctttg caagaattta tttcttaaca ttcctaaaaa attaaaaaac agtatgatta
     1081 cttgccctgt cgacttcatc tataagcagt acaacctttc tgccggattc cttcacaaaa
     1141 cgggtaattg caccggagat ctcctcgaaa ctttttagac ctttgccgaa ttcttgaaat
     1201 ttctgtctaa agtcctcatt ctcataaatg agtcccagtg aaaaactgta aaaaatatgg
     1261 tcttgctgga ggcctgcagt aatttatcag cagtcattat tataaacttt ttctgcaggc
     1321 agaactgaaa ttaaactctt gactgtcaaa tctgcactca gcggaattca aaaagacagc
     1381 ataggagcag gcaatatgtc ggcagtgaaa aaaaatacag tgaaatttga aacgcatctg
     1441 aaaaagggaa atgattattt cagcgatgca gactatccga aggcgctcag ggaatatgca
     1501 aaggcagtcg aactggactc aggcagtgca gaggcatatt acagaatagg atcggcatat
     1561 ttcaatcttg cggagtaccc gaatgcagtt cagaattata cagagtgtct gaaaatagac
     1621 ccgaaacaca ttcaggcata tattgagaga gctggtgcat atgccaatct gggagatctg
     1681 gattcttcaa ttgaagatct taagaccgtt ctcaaactgg ataaaaatca ttcagatgcc
     1741 cgatttgctc tgaagcaggg agaggaactg aggaagatgg cagcaaaact ctgttcagca
     1801 gttgataaga aaattgataa aaactcaaat gatccgtttc tttacaatga aaagggtctg
     1861 attctgcaga aagcagggga gcatattctt gccgtcagag aattccggaa agcactgaat
     1921 ctgaaaaaag attatgcgga ggcggctgta aactgcggga tctcactgta ctggaatcag
     1981 gactggaagc tctgcattga tatgatgaca tctgc
//
LOCUS       DAOQGJ010000688         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38364_length_2014_cov_2.103114, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000688 DAOQGJ010000000
VERSION     DAOQGJ010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38364_length_2014_cov_2.103114"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="PK683_11050"
     CDS             complement(<1..1961)
                     /locus_tag="PK683_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09031.1"
                     /translation="MALLNAKVDKDTRKKAEKQAFRNAFSAGSIISPAFGEMLNSVRD
                     IPVLKEIVKPTVSLVSNLNAEAAYGVGRVVENIIPEGRLVFGNEYKNWQNHIQRSANA
                     AADSFKSRDLQRDLNAGKTDYRNEVKGMIKEELFKYFANLISPSFPGIRNNDIIKFMK
                     YDQNRRDSAEKERSAERRRQEIAAQTAAAAVLTFVPGAQGAAGAVFSGIVSALESVPV
                     LGNLVTGMKWIIQGAAEYMAEAGSYLQSSIGIAASESAGAGAAGTAANSLTAQALQNY
                     SSASILTSGLSAAADGNSEGVIAAAVNGLLLSFTGPAGGFAGHVSYTPPEKTDLLTGL
                     LNKIDGASKPTSGWGGGFNFGGSIFNAGLTFTPGSGTSVDLGFSNGGQWNQSMFGNIS
                     YNINSGHTNGSIGLGQEYGTNLSANFSTDEAPSYTLSFGCDMQGDKGKTGTNCGGGGA
                     NKYGLGGSLTLTENGIDAGIDLLGNSFANSSYDRESGTWSDPEFDESWAYNVQQMSSQ
                     DLADEEINASARKTVFDEHYKKLLENDPSIAERLQAFLDGRKPGEDEYTALQSFYQDN
                     RELAAQNGFDLPSDNKNSDSSIADAVYNASQIFLGALGISTLGLGLLNRTGAGTGGSP
                     SPDPRSPIPETEVRPNRREEGDGGVEATP"
BASE COUNT          441 a          511 c          451 g          611 t
ORIGIN      
        1 tcgggcgttg cctcaactcc gccgtcaccc tcttcacgtc tgttcggtct gacttctgtt
       61 tcagggatcg gggatctggg gtcagggctt ggtgaacctc ctgttccagc accagttctg
      121 ttcaacagtc ccagtccaag agtggagatt ccgagtgcgc cgagaaatat ctgggaagca
      181 ttgtaaacag catctgcaat ggaactgtct gagtttttat tatcagatgg cagatcaaat
      241 ccgttctgtg cagcgagttc cctgttatcc tggtaaaaac tctggagggc agtgtattca
      301 tcttcacccg gttttcttcc gtcaaggaaa gcctgaagtc tttctgcaat agaaggatca
      361 ttttccagca gttttttata atgctcatcg aatactgttt ttcttgcgga ggcgttgatt
      421 tcttcatctg ccagatcctg actggacatc tgctgcacat tgtatgccca gctctcatca
      481 aattcaggat cagaccaggt tccgctttct ctgtcatagc tggaatttgc gaaggaattt
      541 ccgagaagat caatgccggc atcgattccg ttttctgtaa gagtaaggga acctccgagt
      601 ccgtatttat ttgctccgcc gcctccgcag tttgtccctg tttttccttt gtccccctgc
      661 atatcgcatc cgaaggaaag agtgtagctc ggggcctcgt ctgtgctgaa atttgcggac
      721 agattcgttc catattcctg tccaagtccg atgcttccgt ttgtatgtcc tgagtttata
      781 ttataactta tatttccgaa catactctga ttccactgtc ctccgttgga aaatcccaga
      841 tcaacagatg ttcctgatcc tggagtgaat gtcagtcctg cgttgaatat ggaaccaccg
      901 aaattgaatc ctcctcccca tcctgacgtc ggtttcgatg caccgtcgat cttattcagc
      961 agacctgtca gtaaatcagt cttttcagga ggagtatagg aaacatggcc tgcaaaacct
     1021 cctgcgggcc ccgtgaagga aagcagtaac ccatttacgg ccgcagcaat gactccttcg
     1081 ctgttcccgt ctgccgctgc ggaaagaccg gaagtgagga tggaagcaga gctgtaattc
     1141 tgaagtgcct gcgcagtgag tgaatttgcc gcagtgcctg cagctccggc acctgcactt
     1201 tcagatgctg caattccgat ggagctttgg agataggatc ctgcctctgc catgtattca
     1261 gcggctccct gtatgatcca tttcattccg gtgacaagat tcccaaggac aggcactgac
     1321 tcgagtgctg atacaattcc ggaaaataca gcacctgccg caccctgtgc accgggaacg
     1381 aaggtcagca ctgctgcagc tgctgtctgt gccgcaattt cctgtcttct cctttccgca
     1441 gatctttcct tttcggcaga gtctctccgg ttctgatcat atttcataaa ctttataata
     1501 tcattatttc gtattcctgg aaaggaagga ctgatcagat ttgcaaagta tttgaaaagt
     1561 tcttccttga tcattccctt cacttcattc ctgtaatctg ttttccctgc attcaggtct
     1621 ctctgaagat ctctgctctt gaatgagtct gcagcagcat tggctgatct ttgaatatga
     1681 ttctgccagt ttttgtattc atttccaaaa acgagcctgc cttcagggat aatattttcg
     1741 acgactctgc cgactccgta tgcagcttcc gcattcagat tggaaacgag agagactgtc
     1801 ggcttgacga tctctttaag tacaggtata tctcttactg aattcagcat ttctccgaat
     1861 gcaggactga tgatgcttcc tgcagaaaag gcatttctga aagcctgttt ttccgctttt
     1921 tttcttgtat ctttgtccac tttcgcattc agaagagcca tggcaatttc ggatgcctga
     1981 catggttctt tggcctttgc actgtcgcct ggac
//
LOCUS       DAOQGJ010000689         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38388_length_2013_cov_2.894791, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000689 DAOQGJ010000000
VERSION     DAOQGJ010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38388_length_2013_cov_2.894791"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PK683_11055"
     CDS             <1..773
                     /locus_tag="PK683_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09032.1"
                     /translation="KMKEKAEKIMGALNKSLVFLVFDINFVLIFGKSRNEKMKLKALI
                     LLLTLCTSVYATDTATVINADGTVKIEPLSKEKERVMEALKNDIKFFHEKLGGIMAFI
                     AKNKKEMIQKEKFSRRELAYIKHETGEQFTFVMNQTTEIQVAGEKISGIKFVFRKGSM
                     RPQEDTNTVIKELFNPFSDDPKSIKMTVFHFPPAHKLANPIGTYGIDDVQNHLDKLEL
                     VRQYKENVRRTVRDLEQLIEHDFTQKGMVIWKSLNEFN"
     gene            815..1156
                     /locus_tag="PK683_11060"
     CDS             815..1156
                     /locus_tag="PK683_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH09033.1"
                     /translation="MMNFTHEIINDFHIFKVTGSLDIYTAVDLKSSIEEAIKDPHSKL
                     AVHMQNIKYIDSSGIGALIKLYNYMKNLNGVFYLVEVPAPQEKVFKVSGLLNYFKFLS
                     EKDFAALIGSA"
     gene            1131..1985
                     /locus_tag="PK683_11065"
     CDS             1131..1985
                     /locus_tag="PK683_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNH09034.1"
                     /translation="MQPSLVQRDPFEIIADIISEIKSQAREDWYLEHTRIIEILGIKK
                     EDYYRIFYSLKDAGFNLTAMKGFSNEEVIHLIHLIDKVLDIGFTDKEFERRGICFSDE
                     FEEELLTYFIDSIEKIFQLHKLDKELLMLLASSTKEFNDAFDSYFEDKFAMEFIISRT
                     VDSFLEKKEIEADIYAENILKNFLNVKSKIRNDRLRSIKTEYRDRFYFELYGRERKVR
                     RAVSVLTPEQRVLYNYFGLQADHGKKELKKKYKELLKQYHPDINKSGLAKTKEIIENY
                     NKLNAMMG"
BASE COUNT          730 a          343 c          382 g          558 t
ORIGIN      
        1 aaaaaatgaa agaaaaagca gagaaaatta tgggtgcatt gaataaatcg cttgtatttc
       61 tggtgtttga tattaatttt gtactgattt ttggtaaatc aaggaatgaa aaaatgaaac
      121 tcaaagcact tatactgctt cttactctct gcacatcagt ttatgctaca gatacagcta
      181 cagtaatcaa cgctgacgga actgtaaaaa ttgaacctct cagcaaagaa aaagaaagag
      241 taatggaagc tctgaaaaat gacatcaaat tcttccatga aaaactcggc gggatcatgg
      301 catttatcgc taaaaataaa aaagaaatga ttcagaaaga aaagttttcc aggagagaat
      361 tggcttatat taaacatgag acaggcgagc agtttacttt cgtgatgaac cagactacgg
      421 aaattcaggt tgcaggcgag aaaatttccg gaataaaatt tgtttttcgc aaaggatcaa
      481 tgagacctca ggaagataca aatactgtca tcaaggaact ttttaatcct ttctctgacg
      541 atccaaaatc catcaaaatg actgtttttc atttccctcc agcacataaa ttggcaaacc
      601 caattgggac ttatggaatt gatgacgtcc agaaccacct cgataaactg gaactggtca
      661 gacaatacaa agaaaatgtc agaagaactg taagagactt ggaacagctg atcgaacatg
      721 actttactca gaaaggtatg gttatctgga aatctctgaa cgagtttaac tgaaataaat
      781 cttcgggcag agttctctgc ccgacaaaat atctatgatg aattttactc atgaaataat
      841 caatgatttc catatattta aagtaacagg cagtctggat atctatactg ccgttgatct
      901 aaaatcatcc attgaagaag caataaaaga tccccattcc aaacttgcag ttcatatgca
      961 gaatatcaaa tatatagatt catctggaat cggggcactc ataaaactgt acaattatat
     1021 gaaaaacttg aacggagttt tttatctggt agaagttcca gctcctcagg aaaaggtatt
     1081 taaggtatcc ggtctcctga attattttaa gtttctatcg gagaaagatt ttgcagccct
     1141 cattggttca gcgtgatcct tttgaaatca tagctgatat catttcagaa atcaaatcac
     1201 aggccaggga agactggtat ctggaacata ctagaattat agaaatactc ggaataaaaa
     1261 aagaagacta ttacagaata ttttacagcc taaaggatgc aggattcaat ctcactgcaa
     1321 tgaaaggttt ttcaaatgaa gaagtcattc atctgattca tctgatcgat aaggttctgg
     1381 atatcggttt cactgacaaa gaatttgaaa gaagaggaat ttgcttttct gatgaatttg
     1441 aagaagagct tctgacttat ttcattgatt ccatagagaa aatatttcag cttcataaac
     1501 tggacaagga acttctgatg ctgctggcgt cctccacaaa agaattcaat gatgcatttg
     1561 attcttattt tgaagacaaa tttgcgatgg agtttatcat ttccagaact gttgattctt
     1621 tccttgaaaa aaaggaaatt gaagcggata tctatgcgga aaatattctc aaaaattttc
     1681 tgaacgtaaa gagcaagatc agaaatgaca ggcttcgttc aatcaaaacg gaatacagag
     1741 acaggtttta ttttgaactg tacggacgtg aaagaaaggt cagaagggct gtttcagtcc
     1801 tgacaccgga acagagggta ctttataatt atttcggtct tcaggcagat cacggaaaaa
     1861 aagaactcaa gaaaaaatac aaagagcttc tgaagcagta ccatcccgac atcaacaaaa
     1921 gcggactcgc aaaaaccaaa gagatcatag aaaattacaa taagctcaat gcgatgatgg
     1981 gatagttttt ccgatatttc agaaatctga ccc
//
LOCUS       DAOQGJ010000690         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38391_length_2013_cov_2.771706, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000690 DAOQGJ010000000
VERSION     DAOQGJ010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38391_length_2013_cov_2.771706"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PK683_11070"
     CDS             complement(<1..903)
                     /locus_tag="PK683_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09035.1"
                     /translation="MENFLHKFRTKLICSAEEKKTLLPLAVLFDTLSEKARREGLNAL
                     EEDLYKINDWLIREGLIMVCSGTSADILQTIFENHLVSGDYSAVSFLGKIIALEGILL
                     IQSADTPRTVRLILASLYGEQAEFVYSSAEKEFNSNFPDNDHIPGLEGEKEVIEEVQE
                     FMENLSSLEESLPEFEPEEMPVPENQNLINTAEIYTVLKDDLRCSLDDKISLIPFVEK
                     LSSIAEKSNKSGFIVLEKDLKGIESWYLRTGLEIIAEGLEFSILESFLLNNLYTSEYR
                     GARLMEKFLVFNSLVLLKAGYLPSM"
     gene            1290..1682
                     /locus_tag="PK683_11075"
     CDS             1290..1682
                     /locus_tag="PK683_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNH09036.1"
                     /translation="MTDQEMEIRMQKLGIREEDLQETFILSQGKGGQNVNKTSTAVRL
                     VHIPTGTEVKCQKERSQVMNRLRARELLCDKIESKIMKEKSLREQAFEKSRRQKRKRS
                     KSAKEKILKDKAAQSRRKKERAKVRDAE"
     gene            1771..2004
                     /locus_tag="PK683_11080"
     CDS             1771..2004
                     /locus_tag="PK683_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09037.1"
                     /translation="MYDKTLVLEILLQIKEAAERIINRADKIHSAQEFYDSDANRDKL
                     DSICMMLIAIGESLKKIDKITDGKLLSGYPQVD"
BASE COUNT          622 a          382 c          458 g          551 t
ORIGIN      
        1 cattgatgga agatatcctg ccttgagaag aacaagggaa ttgaagacca ggaatttttc
       61 cataagcctt gctcctctgt attctgaagt atacagattg ttcagaagaa aactttccag
      121 tatgctgaat tccagtccct ctgcaatgat ttccagccct gtccgaagat accaggattc
      181 tattcccttc agatcctttt ccagtacaat gaatccggac ttgtttgact tttccgcaat
      241 agaacttaat ttttctacga aaggaattaa tgatatttta tcatccaggg agcatctgag
      301 atcatctttc agaacagtgt agatttctgc tgtgttgatc agattctgat tttccggtac
      361 aggcatttcc tcaggttcaa attccggaag gctttcctca agagaagaca gattttccat
      421 gaattcctga acttcctcaa tcacttcctt ttccccttcc agtccgggga tatggtcatt
      481 gtccggaaaa ttggaattga attctttttc tgcggaggaa tagacaaatt cggcctgttc
      541 tccgtaaaga gaggcaagaa tcagacggac tgttctgggc gtgtctgcgg actgaatgag
      601 aagaattcct tcgagtgcaa tgatcttgcc cagaaaggaa actgcagaat agtctccgga
      661 caccagatga ttttcgaaaa tagtctgcag aatgtctgct gaggtgccgc tgcagaccat
      721 gatcagtcct tcacggatca gccagtcgtt gattttgtac aaatcttctt cgagtgcatt
      781 cagtccttcc cttcttgcct tttccgagag ggtatcgaac agaactgcca ggggaagaag
      841 agtctttttc tcctcggcag agcagatcag ttttgtcctg aatttatgca gaaaattttc
      901 caaaagtcca cctctattgc tcagatttcc gacagcttgg tcggcaaaac aggaagctgc
      961 ctttcccgct ttcttctgac tatcagtgaa aatgcaattt tactttcagt caatgattaa
     1021 ttaactgtaa gaaaatttca ctgtaaatcc gagtatttat cctttttcgt ttatgtcctg
     1081 aaaatagcag tattttttct gtctgatgag tgaaaatgag cgccgcataa aaatatgtct
     1141 gaactgaaaa gatttccgtt tttgcactcc gaaaagacac tgtgtttatc tgcagtaaaa
     1201 ctgtttttac agagcgcgga tattttttat acgcttatgc agaaaaatca gtctgagtaa
     1261 aatagagctg cgaaacaggg agacagagga tgacagatca ggaaatggaa atcaggatgc
     1321 agaagcttgg catacgtgag gaagatctgc aggagacatt cattctttct cagggaaagg
     1381 gcggacagaa tgtgaacaag acttcgactg cggtgaggct tgtgcatatt cctacaggaa
     1441 cagaggtgaa atgtcagaag gagcgttcac aagtgatgaa ccgacttcga gcaagagaat
     1501 tattatgtga taaaattgaa tcgaaaataa tgaaagaaaa gtctctgcgt gaacaggcat
     1561 ttgagaaatc ccgcaggcag aaaaggaaaa gaagcaaaag tgcaaaggaa aagattctga
     1621 aggacaaggc cgcccagagc cgcaggaaaa aggaaagagc gaaagtgaga gatgcggagt
     1681 agggtgcgaa tcactcccga gtagatctga ttcagtacag agaaagaatg aatcaatttt
     1741 taaagtcaag aattgacaga gagggtatct atgtatgata agacccttgt tctggaaatt
     1801 ctgcttcaaa taaaggaggc agcggaaaga atcataaaca gagcagataa aattcacagt
     1861 gctcaggaat tttatgattc agatgcgaac agggataaac tggattccat ctgtatgatg
     1921 ctgattgcca tcggtgaaag tctgaaaaaa attgataaaa tcacggatgg taaacttctt
     1981 tcgggatatc cgcaggtaga ctgacaggtt tta
//
LOCUS       DAOQGJ010000691         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38392_length_2013_cov_2.728805, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000691 DAOQGJ010000000
VERSION     DAOQGJ010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38392_length_2013_cov_2.728805"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PK683_11085"
     CDS             <1..673
                     /locus_tag="PK683_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09038.1"
                     /translation="YIKNHYKYIMKEIQAGLKSFNELVREFAGETKADQYIHLKRHEK
                     VRELLRCSDRAVRKGALWAFAENPREEYKASTPGIRLISGLDKKPFVKGEVFLRSFIL
                     QLQDFDSEFRRFAGSFLSHLKWNKKFLPFISQIPKHLHTPELTECLMERIYDLKVLTG
                     YLSDTEPEKRRIALSRVYDLCDNEDTIQKMISEIQRVKGEKTDEMNSFLSNPEAGIPE
                     YDPYD"
     gene            complement(703..1047)
                     /locus_tag="PK683_11090"
     CDS             complement(703..1047)
                     /locus_tag="PK683_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09039.1"
                     /translation="MELVKYSRCFFTGLFCLLTACQKSSPRYWNEYYIDREREKQADR
                     TVYREASCPALKNCYSECAESRWVRNTDSRGRAVVRNTFRDGTKGSFWNPETLTECER
                     KCRTEIFCTDKD"
     gene            complement(1035..>2013)
                     /locus_tag="PK683_11095"
     CDS             complement(1035..>2013)
                     /locus_tag="PK683_11095"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNH09040.1"
                     /translation="VSFEDGRTETVSKRDLKKFRFETAIWKRNESAEGQHSEEERLSE
                     QAKDGNEWVERPEDEKISPLGNAALGLIPGYSGLYRTENYSGGLGFTVIEIGLVGLLA
                     SYSVPAGNENSDAKAVRNSQLSTAAGGLGAVLLMDSMFSYYSAKNWNEGRYQGKKADP
                     YARPTTVFSRFWRSALVPGWGQVYAGNTVKGGFFLGIAGLLGAGILSLNPVRNNAEEK
                     VKNNENMVMYALTAPSSSSSAGLLRISFNQYNSSKVELDSRKEEESMILSGIALLWML
                     NIADAVFFSGKKAEETAEKRLIFQPEFRMNRVMNAGLETNIGGRISWNW"
BASE COUNT          565 a          485 c          391 g          572 t
ORIGIN      
        1 atacataaaa aatcattata aatatatcat gaaagaaatt caggctggtc tgaaaagttt
       61 caatgaacta gtgcgtgaat ttgcaggcga aacaaaagct gaccagtata tccatctgaa
      121 acggcatgaa aaagtcagag aacttctgag atgcagtgac cgggctgtcc gcaaaggagc
      181 tctctgggcc tttgcggaaa accctaggga agaatacaag gcctctactc ccggaataag
      241 gcttatttcc ggactggata aaaaaccttt cgtaaaaggt gaagtattcc tccgttcatt
      301 cattctgcag cttcaggact tcgattccga gttcagacgc tttgcgggaa gttttctttc
      361 ccatctgaaa tggaataaaa aattccttcc cttcatttcc caaataccga aacatcttca
      421 tacgccggag ctcacagaat gtctgatgga aagaatctac gatctgaagg ttctgaccgg
      481 gtatctttca gatactgaac cggaaaagag aagaattgca cttagcagag tctatgattt
      541 atgtgataat gaagatacaa ttcaaaaaat gatttcagag atacagaggg tcaagggaga
      601 aaaaacagat gaaatgaatt catttctgtc caatccggag gcaggcattc ctgaatatga
      661 cccttatgac tgaagagcag ttccaatgct ctcagccccg ggtcaatcct tatcagtgca
      721 gaaaatctct gtcctgcatt ttctttcgca ttcagtgaga gtttcaggat tccagaaact
      781 tcctttcgtc ccgtcccgaa atgtatttct gaccactgcc cttcccctgc tgtcagtatt
      841 cctgacccat ctgctttcgg cacattcaga ataacagttt ttcagcgcag gacaggaagc
      901 ttctctatac acagttctgt ccgcctgctt ctctctttct ctgtcaatat aatattcatt
      961 ccagtatctt ggactgcttt tctgacaggc agtaagaaga cagaagagtc ctgtgaagaa
     1021 acacctgctg tactttacca gttccatgat attctccctc cgatatttgt ctccaagcct
     1081 gcattcatta ctctgttcat cctgaattca ggctgaaaaa tcagacgttt ttctgccgtc
     1141 tcctctgctt ttttgccgct gaaaaaaacc gcatccgcaa tgttcagcat ccagagaagg
     1201 gcaatcccag agagaatcat ggattcttct tcctttctgg aatccagttc aactttggat
     1261 gaattgtact gattgaagct tattctcaga agacctgcag aagaagagga agaaggagca
     1321 gtgagagcat acatcaccat attttcatta ttttttactt tttcttcagc attgttcctg
     1381 acaggattca aggacagaat tccagcacct aaaagtccgg caatgccgag aaagaatcct
     1441 ccctttactg tatttcctgc atagacttgt ccccagccag gaaccagggc agatctccag
     1501 aatctggaaa agacggtcgt cggtcttgca taaggatcag cttttttgcc ctggtacctg
     1561 ccttcattcc agttttttgc agaataataa gagaacatag aatccatcag cagtaccgca
     1621 ccgagtcctc cagcagccgt cgaaagctga gagtttctga ctgcctttgc atctgaattc
     1681 tcatttccgg ccggaacaga ataggaagcc aaaagaccga caagcccaat ttcaatcaca
     1741 gtaaaaccga gtcctcctga ataattctca gttctgtaaa gcccggaata tcctggaata
     1801 agtccgagag cagcatttcc gagaggactg attttttcgt cttccggtct ttccacccat
     1861 tcattcccgt cctttgcctg ctcagacaga cgttcttctt ctgaatgctg tccttcagct
     1921 gattcatttc ttttccatat cgcagtctca aatctgaatt ttttcagatc ccttttcgaa
     1981 acagtttcag ttcgtccatc ctcaaaggaa act
//
LOCUS       DAOQGJ010000692         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38461_length_2011_cov_2.799591, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000692 DAOQGJ010000000
VERSION     DAOQGJ010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38461_length_2011_cov_2.799591"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PK683_11100"
     CDS             complement(<1..459)
                     /locus_tag="PK683_11100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH09041.1"
                     /translation="MLKDRLEENYERLKKIGLIRKTEAFRVYDNETEDYPCTIDLYSD
                     YAYISLFEKKNIDMEDLALAVKYSLKNTFNTADEKCVIRIRRRQADGSQYEKLGSSGT
                     TFTVKELGVKYYINLWDYLDTGLFIDHRKTREIFAGLVKGKRRVLNLFSYT"
     gene            complement(440..1060)
                     /locus_tag="PK683_11105"
     CDS             complement(440..1060)
                     /locus_tag="PK683_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09042.1"
                     /translation="MIDFHYTTEENGVRLQLKTDMTEVGTFHRMVTVLYALDWNIVSG
                     EVRTSEKDGKTFADNTLLLSDEDGKKKTKVIEIGILMDTVFSAKASLEEILYEYTIPK
                     VDPKKFFGKDFELIFFDEPKAGMTCFHIEAYSAKGLLYHVTKALADEKINIVSGLIET
                     DEKTGKANDTFYLLDSQNRMFASDSVTERIRQRIWNTSREYVKRQT"
     gene            complement(1057..>2011)
                     /locus_tag="PK683_11110"
     CDS             complement(1057..>2011)
                     /locus_tag="PK683_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09043.1"
                     /translation="YSTSERFHRKLESYFSDAQSSGFFGAKTLAVFPDHIGTFLFLAG
                     EKRSVYSAENMGSALSLIASSRPLSYMRHRITSFSESKQLESVIRLKSESVRDIYTAV
                     FSKLASVYQVSILAGSVVLPGAYVQNGKIYVSPAAELKTYSFVFLPDGKVSDTFFVKK
                     TPSVWEKNLGIKEETDKTVTSKLAGTVDSWVAMTEDSLYNSAYSSLSTGFEVLISPSF
                     LYKDTQIQFSKPEISIDPRTGKSLMNDSDHSAPLEILWDKFSLPGKINASIGRIYMQV
                     FMAGKIYEYTLSAPSKASIKYNKTESVDGNRFRSAVLNIWL"
BASE COUNT          560 a          431 c          382 g          638 t
ORIGIN      
        1 tgtataagaa aacagattca gaactcttct tttgcctttt accagaccgg caaatatttc
       61 ccttgttttt ctgtgatcaa tgaaaagccc cgtatccaga taatcccata aattaatgta
      121 atattttacc ccaagctcct ttactgtaaa tgttgtcccg gaagaaccca gcttttcata
      181 ctgacttccg tccgcctgac gccttctgat ccggattaca catttttcgt ctgcagtatt
      241 gaatgtattc ttcagggagt atttcactgc aagagcgaga tcctccatat caatattttt
      301 cttttcaaaa agacttatat atgcatagtc tgaatacaga tcaattgtgc atggatagtc
      361 ttctgtttca ttgtcataaa ctctgaatgc ttctgtcttt ctgatcagtc cgattttttt
      421 cagacgttca taattctctt caagtctgtc ttttaacata ttcccgtgag gtattccaga
      481 tcctctgcct gatcctttca gtgacggaat cggatgcaaa cattctgttc tgactgtcga
      541 gaagataaaa agtatcgttc gcttttcctg tcttctcgtc agtttctata agtccactta
      601 ctatattgat tttttcatca gcgagagcct ttgtgacatg ataaagaagt ccctttgccg
      661 agtaggcttc aatatggaaa caggtcattc ctgcctttgg ttcatcaaaa aatatcagtt
      721 caaaatcttt tccgaagaat tttttcggat caaccttggg aattgtgtac tcataaagta
      781 tttcttcgag cgaagctttt gcagaaaaga cagtatccat aagtattccg atttcaatga
      841 ctttagtttt ctttttaccg tcctcgtcag agagcagaag agtgttgtct gcaaatgttt
      901 tcccgtcttt ttcagaagtt ctgacctcgc cagaaacaat gttccagtca agtgcataca
      961 gaacagtcac catccggtga aatgttccga cctccgtcat atcagtctta agctgaagac
     1021 ggacaccgtt ttcctcggtt gtataatgaa aatctatcat aaccagatat tcagaacagc
     1081 ggatctgaat ctgttcccgt ccacagattc agttttattg tactttatcg atgcttttga
     1141 gggtgcagaa agcgtatatt cataaatttt tccagccatg aacacctgca tatatattct
     1201 gcctatggaa gcatttatct tgcctggtaa tgaaaattta tcccaaagta tctccagagg
     1261 ggctgagtgg tcagaatcat tcatcagact cttcccggtt cgaggatcaa tagaaatttc
     1321 cggcttggaa aactgaatct gtgtatcttt gtaaagaaag gatggagaaa tcagtacttc
     1381 gaatcctgta gaaagggatg agtaggcaga attatagaga ctgtcttctg tcattgccac
     1441 ccaggaatcg acagtaccag ccagctttga agtcactgtc ttgtcggttt cttcctttat
     1501 tcccagattt ttctcccaga ctgaaggagt ttttttgaca aaaaaagtgt cagaaacttt
     1561 accatccggc agaaacacga aagaataagt cttgagttca gctgctgggc ttacgtaaat
     1621 ttttccattc tgcacatagg ctccaggaag aacaactgaa cctgccagaa tgctgacctg
     1681 atacacagag gcgagcttgc tgaagactgc agtatagata tctctgacag attccgattt
     1741 cagtctgata actgattcga gctgtttgga ttccgagaac gaggtaatcc tgtgtctcat
     1801 atatgaaaga ggcctggagg aagcaataag actgagtgca gaacccatat tttccgcact
     1861 gtagaccgac cttttttcgc ctgcaagaaa cagaaacgtt ccgatatgat ctggaaatac
     1921 agcaagtgtc tttgctccga aaaatcctga actctgtgca tctgaaaaat aagattccag
     1981 tttcctgtga aatctttccg atgttgaata c
//
LOCUS       DAOQGJ010000693         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38469_length_2011_cov_2.390082, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000693 DAOQGJ010000000
VERSION     DAOQGJ010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38469_length_2011_cov_2.390082"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PK683_11115"
     CDS             <1..985
                     /locus_tag="PK683_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09044.1"
                     /translation="RLIQLYSGLSVLNADRKKNLSLLLKSRSPEGERWKELSAASVHG
                     EPSGAVYNDDRLYFHYDFSVLSWNSDSDTLGWRKETKEKIKEIITGRHLLLRMEKSFV
                     LLNSGDGERLWILTPNASAIERAEYGRFIYFSASVANGERYLYRVDPESGIIMNSRRL
                     NSSEDWRVWNDGRIFIMENSDKPGYYFLKHYLNPASRKKYLPFKADSVFISSSGALLE
                     SVKSKAVFIYDENSFTEISLRPSSQTAAVSGRNIFFLEGRKLQSYDAASGRTEVLYTM
                     DQNEKLVSFTSDSFIFMKNREIFIMNFRTDAKEKFYLPASRNKTPLIYLHF"
     gene            complement(1070..1828)
                     /locus_tag="PK683_11120"
     CDS             complement(1070..1828)
                     /locus_tag="PK683_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09045.1"
                     /translation="MIKNIVSVYDRLYENASAETGNRTYFFLAVSIIGGGLFFLSFFL
                     ADANPFRLLLPGSLHDFSFSARDKRKKAEVFLADAQKKVFTSERKVFLSGDFKRDLVS
                     LITEVGEPPYYETEENLNKKDLNTNFKKLPNLHYALITFWKRNGLLILDFREETLNSE
                     MEAIKVDTSEVRYDIAEEGDQTKLAVIQAKEEKKKKRAKELKRELLSSGFLALEKTIF
                     ANFKDIDRIEYRLNGKIKNFPELEYSLTETKKRQ"
     gene            complement(1821..>2011)
                     /locus_tag="PK683_11125"
     CDS             complement(1821..>2011)
                     /locus_tag="PK683_11125"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNH09046.1"
                     /translation="GVKKMNLQVLRGSLMPGVLVELGFLTHPEEARLLADETVQLKIA
                     KGIAEGIKLYVHKRKTND"
BASE COUNT          544 a          420 c          414 g          633 t
ORIGIN      
        1 aaggctgatt cagctgtatt cagggctttc tgttctcaac gctgacagga aaaagaatct
       61 ttcgcttctg ctgaaaagca ggagtcctga aggtgaaagg tggaaggagc tttcagccgc
      121 ctccgtacat ggagaacctt ccggtgcggt ttataatgat gatcggttgt attttcatta
      181 tgatttctcc gttctttcct ggaattctga ttctgatacc ctgggttgga gaaaggagac
      241 gaaggaaaaa ataaaggaaa ttatcacagg aagacatctt cttctgagaa tggagaagtc
      301 gtttgtcctg ctgaattcgg gggacggtga aaggctctgg atactgactc cgaatgcatc
      361 cgcaattgaa agagcggagt acggcagatt catttatttt tctgcatcgg tcgcgaacgg
      421 agaacgttat ctgtacagag ttgatcctga atcaggaatc attatgaaca gcagaagact
      481 caactcatct gaggactgga gagtatggaa tgacggaaga atcttcatta tggaaaattc
      541 ggacaagcct ggatattatt tcctgaagca ttacctgaat cctgcttccc ggaaaaaata
      601 tcttcctttt aaggctgatt ctgtctttat ttcttcgtca ggagccctgc tcgaaagcgt
      661 aaagtcaaag gcagtcttca tttatgatga aaattcattt acagaaatca gcctgagacc
      721 ttcctctcag acagccgccg tttccggaag aaacatattc tttcttgagg gaaggaaact
      781 ccagagttat gatgcagctt caggcaggac agaagtttta tatacgatgg atcagaatga
      841 aaaacttgta tcttttacaa gtgacagttt tatattcatg aaaaatagag aaatattcat
      901 tatgaatttc agaacagatg cgaaggagaa attttacctt cctgcctcca ggaataaaac
      961 acctctgatt tatcttcatt tctgaatggg agtaattgcg tcagtcagcc gccttgcagt
     1021 ctgctgcggc tgtcagacga ctaagttatg gaatgctttt ttgttccgac tactgtcttt
     1081 tctttgtttc agtcaggctg tattcgagtt caggaaaatt ctttatcttt ccgttcagtc
     1141 tgtattctat cctgtctata tccttgaaat tggcaaatat tgtcttttcg agagcaagga
     1201 accctgatga aaggagttct cttttcagtt cttttgctct tttctttttc ttctcttctt
     1261 ttgcctgaat gactgcaagc ttggtctgat cgccttcttc tgcgatgtca tatctgactt
     1321 cacttgtatc caccttgatt gcctccattt ccgaattcag tgtctcttcc ctgaaatcca
     1381 gaatcagcag accgtttctt ttccagaagg taatcagagc ataatgaaga tttggaagct
     1441 ttttaaaatt tgtattcaag tcttttttgt tcaggttttc ttccgtttca tagtaaggcg
     1501 gttcaccgac ttcagtaatc agtgacacca agtccctctt aaaatcaccg gagagaaata
     1561 cctttctttc agaagtgaaa acttttttct gagcgtccgc cagaaatacc tctgcctttt
     1621 ttcttttgtc tctggcagaa aaactgaaat catgcaggct ccccggaagc agaagtctga
     1681 acggatttgc gtcagcaaga aagaaagaca gaaaaaaaag cccacctcct atgatggata
     1741 ctgcaagaaa aaaatatgtt ctgtttcctg tttctgcaga tgcattttcg tacagccggt
     1801 catatacaga gacaatgttc ttaatcattg gtttttcttt tatggacata aagtttaata
     1861 ccttcagcga ttccttttgc aattttcagc tgtactgtct cgtctgcgag aagccttgct
     1921 tcttcaggat gagtcagaaa gcccagctcc acaagtactc ccggcataag actgcctcga
     1981 agcacctgca aattcatctt ctttacacct c
//
LOCUS       DAOQGJ010000694         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38498_length_2010_cov_3.020972, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000694 DAOQGJ010000000
VERSION     DAOQGJ010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38498_length_2010_cov_3.020972"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PK683_11130"
     CDS             <1..1271
                     /locus_tag="PK683_11130"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09047.1"
                     /translation="MKTHSWDYHRKAHTKSVPKKILAVSLIFTLSALFFHWLSFQFGS
                     NFWISVTARLFYIPVIYSAIMGGLIAGSSVGIISGLMHAGLMFSLHSFQHIENTVLFS
                     HLLETVFLLLIGFFTGALRDHEQHEKKLREEISGLLDRYVSPAVAEKLREKKLLQDGE
                     EIKASVLFCDLAGFTSLSETVSPKELFTLLNKFFPEIVDILLSRNAFLDKFIGDAVMA
                     VFGIPFTSEDDADRAVNSAVEIQNRLRKLNEERFFGNHSLRMSIGINSGLVAAGNVGS
                     LSRASYTVIGDNVNLASRIQSLNGIYGTNTLISDETLKSVRNMENFSFREIDSVRVKG
                     RKRPCVIFDLYSGDPENLQKAKRQTESLLMSALNYYKSGDFEKSEKMFLEMQKETAED
                     RIVGIYLERLAVLKKDNPKNWDGVFEFKTK"
     gene            complement(1273..1344)
                     /locus_tag="PK683_11135"
     tRNA            complement(1273..1344)
                     /locus_tag="PK683_11135"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1310..1312),aa:Pro,seq:ggg)
     gene            complement(1457..1939)
                     /locus_tag="PK683_11140"
     CDS             complement(1457..1939)
                     /locus_tag="PK683_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09048.1"
                     /translation="MIDMNTLETFDALKKVNFTEEQSRELVRFFGKALEADPASRRDL
                     SETELRLQKEIAEIRKETEGIRLEIQDVRFEIKNVEVKFLKEIEHTRLEIKNVEMKLS
                     KEIEDTRLEIKELDVRGREAESRIIQTVASSKAEILKWVAGMLIANTGLIVALSKLIK
                     "
BASE COUNT          584 a          421 c          398 g          607 t
ORIGIN      
        1 ttatgaaaac tcactcctgg gattaccaca gaaaggcaca tacaaagtca gtgcctaaaa
       61 agattctggc agtctctctg atttttacac tttctgcact ttttttccac tggctttctt
      121 ttcagtttgg ttccaatttc tggatttctg tcactgccag actgttctat atccctgtga
      181 tttattccgc cattatggga ggactcattg caggctcctc tgtcgggatc atttcaggac
      241 tgatgcacgc aggtctgatg ttttccctcc acagttttca gcatatagaa aatacagttc
      301 tattcagtca tcttctggaa acggtatttc ttctgctgat cggattcttt acaggagcat
      361 taagggatca tgaacagcat gaaaaaaagc tgagagaaga aatctcgggt cttctggaca
      421 gatatgtttc gcctgcagta gcagaaaaac ttcgagaaaa gaaactttta caggacggtg
      481 aagagataaa agcatcagtt ctcttctgtg atcttgctgg attcacttcc ctttcagaaa
      541 cagtttcacc gaaggagctg ttcactcttc tgaataaatt ctttccggaa atagtggata
      601 ttcttctctc ccgaaatgca tttctggata aattcatagg tgatgctgta atggctgtat
      661 tcggcattcc gttcacatca gaagatgatg cggacagagc tgtaaattca gctgttgaaa
      721 ttcagaacag gctcagaaaa ctgaatgagg aaagattttt cggaaatcac agtctcagga
      781 tgagcattgg gatcaattca ggtcttgtcg cagcgggaaa tgttggttct ctctcaagag
      841 catcttacac tgtaatcgga gacaatgtca atcttgcttc cagaatacag tcattaaacg
      901 gtatatacgg aaccaataca ctgatttctg atgaaaccct gaaatcagtc agaaatatgg
      961 aaaatttcag tttcagagaa atagattcag tcagagtcaa aggaagaaaa agaccctgcg
     1021 tcatttttga tctttacagc ggtgatccgg aaaatctgca gaaagctaaa aggcagacag
     1081 aatccttact gatgtccgca ctgaactatt ataaaagcgg tgattttgaa aaatcggaaa
     1141 agatgtttct tgagatgcag aaagaaacag cagaagacag aattgtcggg atttacctgg
     1201 agagacttgc tgttctgaaa aaggataacc cgaaaaactg ggacggagta tttgagttca
     1261 agacaaaata agtcggagtg acaggatttg aacctgcgac cacttgcccc ccagacaagt
     1321 gcgctaccac tgcgctacac cccgtacatt ccagaaaaaa gaaacttgca ttgagatcaa
     1381 gcattttaca gacagacaga attctaccga gagcagattt tggatgactc aatgatctgc
     1441 gaatcatgga aatcagtcac ttaatgagct tgctcaatgc cacaatgagt ccagtatttg
     1501 caatgagcat ccctgccacc catttgagga tttctgcttt cgaggatgcc actgtctgaa
     1561 taatccggct ttccgcttct cttccccgaa catccagctc tttgatttca agacgtgtat
     1621 cttcaatttc ttttgaaagc ttcatctcga cgtttttgat ttcaagacgc gtatgctcta
     1681 tttcctttaa aaacttcact tcgacgtttt tgatttcaaa tcggacatcc tgaatttcca
     1741 aacggatacc ttcagtttcc ttgcgtattt ctgcaatctc tttttgaagt cgaagttcag
     1801 tttcgctcag atctcttctg cttgcaggat ctgcctctaa agcttttccg aaaaacctta
     1861 caagctccct ggactgttcc tcagtgaagt ttactttttt cagagcatca aatgtttcta
     1921 gagtattcat atctatcata taatgatacc tgtccaaaaa atgtcaacat ctgaattttt
     1981 tgttacgttt ttgcatttta tgggaaataa
//
LOCUS       DAOQGJ010000695         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38578_length_2007_cov_3.750512, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000695 DAOQGJ010000000
VERSION     DAOQGJ010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38578_length_2007_cov_3.750512"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PK683_11145"
     CDS             complement(<1..1158)
                     /locus_tag="PK683_11145"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNH09049.1"
                     /translation="MNKDNILPWTKEPFPESVRKEAESALEKIEKGESSPETEGFTVP
                     LEFGTGGMRGVIGNGIGRMNVYTVARAALGLCRYLNLKFKNPTIVIAYDSRRMSKEFA
                     EITAATAAREKIKVKLFGKPTPTPMLSYAVRHYKAQGGVVITASHNPPEYNGFKAYLN
                     DGGQLVPPDDAKIISSIEKIKDWAQVTGISKKSKEYAKYVSAVDAKVFDTYKKKVLSS
                     GIYNSKMDQKLRSKLKVVYSPLHGTGGDYMKSLLNQAGYKNVFLVPEQAKPDGEFPTV
                     QFPNPEEKEALVLSEAFSRKKNADIFIATDPDADRLGIGVRNSEGNYVLLNGNQTGSI
                     LCAYLCEKISAAPKTDKVWHIFKTIVTTDLQENIAKKNKVKIKNVLTGFKYI"
     gene            complement(1268..>2007)
                     /locus_tag="PK683_11150"
     CDS             complement(1268..>2007)
                     /locus_tag="PK683_11150"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNH09050.1"
                     /translation="VGRVFLYAEKDIYLSGGVMGRNGGFIEAGGNIYARFIEQGTVKA
                     KGSIYIESSSFHSDLTAGDSIFLQGERGELIGGNAIAGKSVEASKIGAVVETKTDITV
                     GLPPEIVDELKKMKQEIINREDTLKKIKQSLHKMNETIAAKKEISTDDKNLLKKLIEL
                     EKRYTEGLISVKKDYETVSQEYDSPEHAFVSAEKFIYPKVNISFGRGKTFSTDIRTID
                     GKVFVYLNSEGLPHTNSIPPAVKKDKK"
BASE COUNT          441 a          459 c          409 g          698 t
ORIGIN      
        1 gatatatttg aatcctgtca gaacattttt gatcttgacc ttgtttttct tcgcaatgtt
       61 ttcctgaaga tctgtagtca cgattgtttt gaatatatgc cagaccttgt ctgtcttagg
      121 agcggccgat attttttcgc agagataagc gcagaggatg cttcctgtct gatttccgtt
      181 cagaagaaca tagttccctt ccgaatttct cactcctatt cccagtctgt ctgcatccgg
      241 atctgtagcg atgaaaatat ctgcattttt ctttctggag aatgcctcgg acagcaccag
      301 tgcttctttt tcttccggat ttggaaactg cactgtaggg aattctccgt ccggtttggc
      361 ctgctccgga acaaggaata catttttgta tcctgcctga ttcagaaggg atttcatata
      421 gtccccgcct gttccgtgca gaggtgaata taccaccttg agcttggatc tcagcttctg
      481 atccatttta gagttataga ttcctgaact cagtactttc tttttataag tatcaaatac
      541 ctttgcatcc acagcggaaa catactttgc atattccttg gactttttgc tgattccagt
      601 cacctgggcc cagtccttta ttttttcgat ggaagagatg atttttgcat cgtccggagg
      661 tacaagctgt cctccgtcat tcagataagc cttgaatccg ttgtattcag gagggttgtg
      721 ggaagctgta atcaccacgc ctccctgtgc cttgtaatga cggactgcat aggaaagcat
      781 tggggtaggg gtcggcttgc cgaaaagttt tacctttatt ttttccctgg cagcagtggc
      841 agcagtgatt tctgcaaact cttttgacat tcttctggaa tcatatgcaa tgactattgt
      901 cgggttcttg aatttcagat tcagatatct gcagagtcca agggccgctc tggcaacagt
      961 atacacattc attctgccga ttccgtttcc tatgactcct ctcattccgc ctgttccgaa
     1021 ttccagcgga acagtgaatc cttctgtttc aggcgaggat tctccttttt ctattttctc
     1081 aagagcggat tccgcttctt ttctgactga ttcaggaaac ggttcctttg tccatggaag
     1141 tatattgtct ttattcatac tgatgtcctt tttgtttact cgctttgcgc aggcggagcg
     1201 tctggcagtc tgccgaaaaa tgataattgc tttttatttc agagggagat aatttcattg
     1261 ctaatcatta ttttttatct ttttttaccg caggggggat gctgtttgta tgaggcagac
     1321 cttccgaatt cagatatacg aagactttgc cgtcgattgt ccggatatct gtggaaaatg
     1381 tctttcctcg gccgaagctg atgttcacct tgggatatat gaatttttca gctgaaacaa
     1441 atgcatgctc aggagaatca tattcctgag acactgtctc ataatctttt ttgactgaga
     1501 tcaggccttc cgtatatctc ttttccagtt ctatcagttt cttcagaagg tttttatcat
     1561 ccgtggagat ttccttctta gccgcaattg tttcattcat tttatgaaga ctctgcttga
     1621 tttttttcag agtgtcttca cggttgataa tttcctgttt catctttttc agttcatcca
     1681 caatttcagg aggaagacct actgtgatat ccgttttggt ttccaccact gcaccgatct
     1741 tgcttgcctc aacggatttt cctgcaattg cgtttccacc gatcagctct cctctttctc
     1801 cctgaagaaa aatggagtct cctgctgtca gatccgaatg aaaggaagag ctttcaatat
     1861 agatggaacc ttttgccttc actgttccct gttcaataaa tctggcataa atgtttccgc
     1921 ctgcttcaat gaatccgccg tttcttccca tgactccgcc ggagagatag atgtcttttt
     1981 ccgcatacag gaagactctg cctacgc
//
LOCUS       DAOQGJ010000696         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38588_length_2007_cov_3.049180, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000696 DAOQGJ010000000
VERSION     DAOQGJ010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38588_length_2007_cov_3.049180"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PK683_11155"
     CDS             complement(<1..801)
                     /locus_tag="PK683_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09051.1"
                     /translation="MDLNIPKITTRLWIWFLGIMCTVPVFSHLLKFTQEGERQIQPAI
                     FSLAALPPLLVAAVVYFYIQTVSLKKSLRNVSGSGNGKEKNLINSYPFRISMLIGITN
                     VAIPTIAQIIGLFRGFVFSWEQSFFFWISDLATAVISSTVFYYFAKKEIYPIIKYTDY
                     EPIRLYHKILIPTMSSIMLLLLMSTAGIYKIGVTRNEDFMRQIMSLNLEEAALYLNTT
                     LEKLLLQVDSYAKSETVRDMNLQRIRDYLKDLQEDKEPFVKSFFVSNMD"
     gene            complement(844..1953)
                     /locus_tag="PK683_11160"
     CDS             complement(844..1953)
                     /locus_tag="PK683_11160"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNH09052.1"
                     /translation="MTGRSRFFRKLRKTSSAEEKSEHRFHWSFVISVFLLIFCLDKIF
                     ISETVLARLVHWKRFERISYSTRHHLFSQFLSEDLEKVKKGTRLILILGSSRSAAMNA
                     RQMQEKLDNTVIYNFSIPFPGPSYFYSVLEMFLEKGIRVDSILLEADEFSFLQDSLNY
                     PFRFFHTFDFILKHSPAVSNSGFTFFDCLHFFEYSLFAVLKNPVDFGVLKEKRNPELT
                     EQSVLLFQKANRERNGGIFETFAEKPMTEMELERDSDLKLQMLLQNERNPYPNISQLN
                     FYRKILTVSAERNIPILVYSPPVSPVLKQKLHGKKKETVFVQRYLSAGVSSNVQWLNL
                     SKEDFDCLEFEDSLHLNRKCSLELTARVLGGIPRK"
BASE COUNT          592 a          421 c          411 g          583 t
ORIGIN      
        1 gtccatattg ctgacaaaga aactcttcac aaagggctcc ttgtcttcct gcagatcctt
       61 gaggtaatct ctgattctct gcagattcat atcccggacc gtttcactct ttgcatagga
      121 atccacctgc agaagaagtt tttccagtgt agtattcaga tagagtgcgg cttcctcgag
      181 attcagactc atgatctgtc tcataaagtc ttcattcctg gtcacgccga ttttataaat
      241 cccggctgta ctcatcagaa gaagcagcat aatggaagac attgtgggaa tcagtatctt
      301 gtgatacagt ctgatcggtt cataatcagt gtatttaata ataggataga tttctttctt
      361 cgcgaaatag tagaatacag tggaagagat gactgccgta gccagatcgc ttatccagaa
      421 aaagaaggac tgctcccagg aaaacacaaa acctctgaac aggccgatga tctgagcaat
      481 tgtaggaatg gccacattgg taattccaat cagcatggaa atgcggaaag ggtaactgtt
      541 gatgagattt ttttcttttc cgttccctga tccggaaaca tttctaaggc ttttcttcag
      601 actgactgtc tgtatgtaaa aatagactac agccgcaacc agcaggggcg gaagggcagc
      661 caggctgaaa atcgcaggct ggatctgtct ttctccttcc tgtgtaaact tcagaagatg
      721 agaaaataca ggcactgtac acataattcc cagaaaccaa atccagagtc gggttgtaat
      781 tttgggaata ttcagatcca tttatacgac gatttctctc agttcaggca gtgcgccttg
      841 ttattatttt cggggaattc cccctagaac tctagcggtt aattccagac tgcattttct
      901 gtttaagtgc agagaatctt cgaattccag acagtcaaag tcttccttcg aaagattcag
      961 ccattgtaca ttagacgaaa ctcctgcact cagatatctc tgaacgaaaa ctgtttcctt
     1021 tttttttccg tgaagtttct gcttcaacac aggtgaaacc gggggagaat ataccagaat
     1081 tggaatattt ctttccgccg aaacagtcag aatcttccga tagaaattca gctgggaaat
     1141 attcgggtaa ggatttcttt cattctgcag aagcatctga agtttaagat cagaatctct
     1201 ttcaagttcc atttcagtca ttggtttctc tgcaaatgtt tcaaaaattc cgccattccg
     1261 ttccctgttt gctttctgga agagcagaac ggactgttct gtcagttccg gatttctttt
     1321 ttccttcagc acaccgaagt caacggggtt tttcagtacc gcaaaaagac tgtactcgaa
     1381 aaaatgcaga cagtcaaaaa aagtaaatcc tgaatttgaa actgcaggac tgtgtttaag
     1441 gataaaatcg aaagtatgaa aaaaacggaa aggataattc agtgagtcct gaaggaatga
     1501 aaattcgtct gcttcaagaa gaatcgagtc cactcgaatc cccttttcca gaaacatttc
     1561 aagcacggaa taaaaatagg aaggaccggg aaaaggtatg ctgaaattat aaattactgt
     1621 attatccagt ttttcctgca tctgtcttgc attcattgca gccgatctgg aggagcccag
     1681 gatgagaatg agcctggttc ccttttttac tttttccagg tcttctgaaa ggaactgact
     1741 gaacagatga tgtcttgtac tgtatgaaat tctttcaaaa cgtttccagt gcacaagtct
     1801 tgccagaaca gtttcagaga taaatatttt atccaggcag aaaatcagca gaaatactga
     1861 aatgacaaaa ctccagtgaa accgatgttc tgatttttcc tctgcagaag acgtctttct
     1921 aagttttcta aagaatcggg accggccagt caattagctc ttttctctcc ggaatatcgc
     1981 agatcagcga agctgcagca ctttcaa
//
LOCUS       DAOQGJ010000697         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38650_length_2005_cov_3.367692, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000697 DAOQGJ010000000
VERSION     DAOQGJ010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38650_length_2005_cov_3.367692"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PK683_11165"
     CDS             <1..424
                     /locus_tag="PK683_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNH09053.1"
                     /translation="KTLSLRMDRHCSNALAIAEWLEAQPQIEKVIYPGLKSHPAYETA
                     KKQMSAFGGMITFILKGTIENSRKFLENCSVFSLAESLGGVESLIEHPAIMTHASVPP
                     EIRKELGISDGLIRVSVGLEDKDDLINDFKNALKECGL"
     gene            complement(474..923)
                     /locus_tag="PK683_11170"
     CDS             complement(474..923)
                     /locus_tag="PK683_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09054.1"
                     /translation="MSLQWPNGNVDEMKFISYKEGVFQFEQGATLANTVITKKDGENP
                     EGKCDFFKSGPIDWDRTAMKFGPGILEFNGNVLTAKENGQVFGTWNIEWNPTECSVKG
                     KIVQHLNPKAVGQLTEFKITDIKGKTATFVWRFYSTGEVKLLGQGKQ"
     gene            complement(1596..>2005)
                     /locus_tag="PK683_11175"
     CDS             complement(1596..>2005)
                     /locus_tag="PK683_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNH09055.1"
                     /translation="FLAGFFIRHYMHEFIKEPVLEPQSDGAVTAEGGETAVPPPEIMI
                     RKIFQASRLGDPVTPDEVIFDAKGVTFNVKRIFESTETYIPYSKISGVEVFESFILAS
                     VKIKQLQGTEIKIENFRKDDAKQIKKFILEKLV"
BASE COUNT          568 a          434 c          355 g          648 t
ORIGIN      
        1 aaagactctt tctctcagaa tggacagaca ctgttccaat gcattggcca ttgcagaatg
       61 gcttgaggcg cagcctcaga ttgaaaaagt gatttatccg ggtcttaaat cacaccctgc
      121 atatgaaact gccaaaaaac aaatgtccgc attcggagga atgataacat tcattctcaa
      181 aggcaccatt gagaattcaa ggaagttcct ggaaaactgc agtgtatttt ctctcgcaga
      241 aagtcttggc ggtgtggaaa gtctcattga acatcctgct attatgactc atgcatctgt
      301 tcctcctgaa atcagaaaag aacttgggat atcggatgga ctgatcagag tttcggtggg
      361 attggaagat aaggatgatc tgattaatga ctttaagaat gcattgaaag aatgcggtct
      421 ataaactgaa taaaaaacag ctgttttaca gctgtttttt tcatttcttt tttttactgt
      481 ttgccctgcc ctaaaagttt tacctctcca gttgaataga atctccagac aaacgtggcc
      541 gtttttcctt taatatcagt gatcttgaat tcagtcaatt gaccgactgc cttaggattc
      601 agatgctgca caattttacc tttgacagaa cattcagtgg gattccactc aatgttccat
      661 gttccgaata cctgtccatt ttcttttgct gtaagtacat ttccgttaaa ttccagaatc
      721 ccgggtccaa atttcatcgc tgttctgtcc caatcgattg gaccggattt aaagaaatca
      781 catttgcctt caggattttc accgtccttt ttcgtaatga cggtatttgc gagagttgcg
      841 ccctgttcga attgaaaaac accctcttta tacgaaataa atttcatttc atccacattt
      901 ccgttcggcc attgaagtga cacagtgcat ggactgatcc agttgatctt tgcttcccct
      961 ttcctttttg tcttgttttc aatgtcaaaa acataggttc ccttttcata tttggaaatt
     1021 acagaaaccc cgtcatttac ttcctcaata acttctccgt cactcagttt tgctcaatgc
     1081 tccttcaggt ctgattctgc ttttttgcag ttcgcaaaag aaaatactgc agccaaagtt
     1141 aacactgcag tcagtctgcc gtttaaaaat ccaatactca tccggcacct cttagaaata
     1201 attttcttcc aattcgggga gtacagtaat tatcggttct ctataagaga taatataatt
     1261 tcttttataa ataatttttc aataagaatg aataatttat tgctcgaaga accgggaata
     1321 ttatattgaa ttttacagag gatcgaaatc ttttgacaga cattagggaa ccatagtgga
     1381 aatataaaat ttcccaggaa atgttcaatg gttttttact caaaaggctg ttttcagagg
     1441 ttcccattat agaccgtccc gggccaggtt ttacgaaact ttatgcaact gtatcaagcc
     1501 gcctcaattc attataaccc ctgcagtata ctctaagttc attctgccct tataccgaat
     1561 ttcagtttaa acctctggct taagcaaaaa aatactcaga ccagtttctc caggatgaat
     1621 tttttgatct gctttgcatc atcctttctg aaattctcga ttttgatctc tgttccctgc
     1681 agctgcttta ttttgactga agctaaaatg aaggactcaa aaacctcaac tcctgaaatt
     1741 tttgaatatg gaatgtatgt ttccgtgctt tcaaaaatcc ttttcacgtt aaaagtgact
     1801 cccttggcat caaaaatcac ttcgtccggc gtaacaggat cccctagtct gcttgcctga
     1861 aatattttcc tgatcatgat ttccggaggc ggaactgcag tttctcctcc ctcagccgta
     1921 acggctccgt ccgactgggg ttcaagaaca ggctctttaa tgaattcatg catataatgg
     1981 cggataaaaa aaccggcaag aaaac
//
LOCUS       DAOQGJ010000698         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38689_length_2004_cov_3.508466, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000698 DAOQGJ010000000
VERSION     DAOQGJ010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38689_length_2004_cov_3.508466"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1015
                     /locus_tag="PK683_11180"
     CDS             359..1015
                     /locus_tag="PK683_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH09056.1"
                     /translation="MKHLFFILLVILFSDCRKTESSAAPSANSSGSVPDSHVSSDRRQ
                     YVTVDSLKLRSKPDLKAETVALLEKGDSFYVTEESSNETEYEGNTSRWGKIKFRGKEG
                     WVFLAFVSFDPPSMLSDAEKEKIQKAYEDEVSRQEKELERQMKADPNFQSCSPPGCGI
                     LTAELANAEKGCKKYKREEINALSKENRLEYENLESYKDDYCKYFGMRIQSQCPVGNW
                     "
     gene            1332..>2004
                     /locus_tag="PK683_11185"
     CDS             1332..>2004
                     /locus_tag="PK683_11185"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNH09057.1"
                     /translation="MIQKILLILLLCNILYCRRPSGDLLEIQGGTFRRGKEIARIPDQ
                     SPPNEVSVSSFYYQSKLVTIAEYSEFQKKTGHITGAEKKGYCMTSHEGMKDWEWEKVY
                     GADFRNPFLGNEDPNAKPKPDWPAVCLSYADAAAYCADLGMRLPTEAEWEYAHRAGST
                     SRFPWGEQPEPRGSFLLNYWQGKTHRNAEVKDGFKYLSSVTAFPPNRWGVYDSVGNVW
                     QITLDY"
BASE COUNT          604 a          387 c          481 g          532 t
ORIGIN      
        1 tcagatttcc cgaggcagac tgtatctgaa aactgccgac tcgaagtaaa acggataaat
       61 tgaagcaatt cttctgcttt tgtgcatacg ggatattttg taactgaaat ttaatttcat
      121 ggctgcattt gcagaattag gagaaaagtg aaaaccggac ttttcagtca gtggtgacaa
      181 tggcgacaga atctttgaaa atgcttactg aacgcactat gctgtaaaaa tagaaatttc
      241 tgccgtaccc aaaattgatg aatttctgca aatatccgat cagtcttgat tcctttgaga
      301 tcccaaaaaa cgctcgattt tctgatctgt ctttttgaaa tcatcatagc aggtatatat
      361 gaaacatcta ttttttatat tattggtaat tttattttct gactgcagaa agacagaaag
      421 cagcgccgct ccttctgcaa attcatccgg atctgtgcct gattctcacg tttcttcaga
      481 cagaagacag tatgtgactg tggattcatt gaaacttcgt tccaagccgg atctgaaggc
      541 ggagacagtc gctcttctcg aaaaaggaga ctctttttat gtgacggaag aatcttcaaa
      601 tgaaactgag tatgagggaa atacaagccg atggggaaag atcaaattca gaggaaaaga
      661 gggatgggtg tttctggcat ttgtttcctt tgatccgcct tcgatgcttt cggatgcaga
      721 aaaagaaaaa attcagaagg catacgagga tgaagtcagc agacaggaaa aagaactgga
      781 gaggcagatg aaggcagatc cgaattttca gagctgttct cctcccggat gcggaatatt
      841 gactgcggaa ttagcgaatg cagaaaaggg gtgcaagaaa tacaaaagag aagaaattaa
      901 tgcactttcc aaggaaaacc ggctggaata tgaaaatctg gagtcttaca aggatgatta
      961 ctgcaaatat ttcggaatgc ggattcagtc tcagtgccct gtcggaaact ggtaactcac
     1021 cttacgcaaa tatgatatgc tgtctagaag cggtattttt ttgaaagcat tagtgaagtc
     1081 taatgaatca gtaaaaacag catcttccaa gcttgcggga gtttagccag tttctaaatg
     1141 ttggcagcca gtcactgaca tcggcgaggg cggtagccgc ttccatacct tgcgagccct
     1201 caagccgccg gcaggcgttc ctgcccaaaa cgctccacct gcagctgaag gcgtaaggtg
     1261 agctggtaaa tatgaatgat gtgaatatca tcatgacttt ttattaaaaa atattctgca
     1321 aggaatctgc tatgattcaa aaaattcttc tgattttact gttgtgcaat attttatatt
     1381 gcagaaggcc ttccggagat ctgcttgaga tccagggagg aactttcaga agggggaagg
     1441 agatcgcacg gattccggat cagtctcctc cgaatgaagt ttccgtttct tctttttatt
     1501 atcagtcaaa actggttaca attgcagaat actctgaatt tcagaaaaag acagggcaca
     1561 ttaccggggc ggaaaaaaaa ggatactgta tgacttctca tgaggggatg aaggactggg
     1621 aatgggagaa agtttacggt gccgatttca gaaatccctt tcttggaaat gaagacccga
     1681 atgcaaaacc gaaaccggac tggcctgcag tatgcctgag ttatgcagat gcggcagcat
     1741 actgtgctga tcttggaatg agactcccta ctgaagcaga atgggaatat gcccacagag
     1801 cggggagcac ttcccggttt ccctggggag aacagcctga gcctcgggga agttttcttc
     1861 tgaactactg gcagggaaag actcacagaa atgcagaagt caaggacgga ttcaagtatc
     1921 tttcttctgt aactgcattc ccgccgaaca gatggggagt ttacgattca gtcggcaatg
     1981 tatggcagat cactctggac tatt
//
LOCUS       DAOQGJ010000699         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38717_length_2003_cov_3.582649, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000699 DAOQGJ010000000
VERSION     DAOQGJ010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38717_length_2003_cov_3.582649"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            658..1614
                     /locus_tag="PK683_11190"
     CDS             658..1614
                     /locus_tag="PK683_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNH09058.1"
                     /translation="MSGNSEKIQSALNEIKKGLVGQEEFAEGLLLSVISDGHLLIEGL
                     PGLAKTRAVNLLAKVCSLGFKRIQFTPDLLPADIIGTRIFNQSRADFEISKGPVFSNF
                     ILADEINRAPAKVQSALLEAMQEKQVTISDTTFPLPKPFFVFATQNPVEQEGTYPLPE
                     AQLDRFIMKINVEYPSQKEESQIVRMIMNEVSLPEVKKVLEPQDILNLQSEARKVHIE
                     EKIISYITDITQATRRPSEFRLDLSDIIQYGVSPRASIAMAVLSRARAFIKGRNAVLP
                     EDVKAVAFPALRHRLILTYHAEAENIRPDAVISKILDNLKVP"
     gene            1611..>2003
                     /locus_tag="PK683_11195"
     CDS             1611..>2003
                     /locus_tag="PK683_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNH09059.1"
                     /translation="MISEKDLLQRVRNLELVGRKNAHSLLNGEYSTKIPGNGLQFHEA
                     RKYVSGESIRLIDWNITARQGEPYVKVLLEEREREIVIALDLSQSMHSGFQDRTKLEY
                     AVELAASVAVSAADSRDRVGCILFTDRTE"
BASE COUNT          554 a          432 c          476 g          541 t
ORIGIN      
        1 tccggaaata tttttttcat ccgaatggat tttattgttt catcctgaaa aactgcggtt
       61 ttgcaggatg tgcagttctc gggcgaggaa tgaacccatt ctttttcaaa tccctctctc
      121 acataaattt tcgacttgga caggtcgact ggaaaagccg gaaggcctgc aggcagcagc
      181 aggagcagtt gaaggatcac aatcagcata tgtcgaatgt tgaaattaat cttctgtgca
      241 gccgaagaac acataaggga tatcactcat actgacataa accggaaggc tgaaataaat
      301 caacagcaaa atattctgca tcagtatatt tgtacgttcg ggattttgcg gaacttttaa
      361 tcagaccttc gtaaatgaat cttggtttaa tcatataagt ttgcatatta tctcatctta
      421 tgcagactgg gcgtttcagg cagtcctcca agggcagctg ctgtccctgg aatctgcaga
      481 tgagacttgc tctgattgag ttctgtatca cgcatttgtc tctctactgc cgcgaagatc
      541 cgcagtttcg ctatgaatcc aacttgtgct ttttctgact tccttcagaa gaggtgattt
      601 ttccattgac tgatccggct cagtacggag aatatacact gctttcggag ggaaaaattg
      661 tctggaaaca gtgaaaaaat tcagtcagca ctgaatgaaa taaaaaaagg tcttgtgggt
      721 caggaggagt ttgcagaagg gctgcttctt tccgtgattt cggacggaca tcttctcatt
      781 gagggtctgc cggggcttgc aaagaccagg gcagtgaatc ttctggcaaa ggtctgcagc
      841 ttaggtttta aaaggattca gtttactcct gaccttcttc ctgcggacat tatcgggaca
      901 agaatattca atcagtcacg ggccgatttt gaaatatcga aggggcctgt gttttccaat
      961 tttattcttg cagatgagat caacagggct cctgcaaaag tgcagtccgc acttcttgaa
     1021 gcaatgcagg aaaagcaggt gactatctct gacactacgt tccctctccc aaagcccttt
     1081 tttgtatttg caactcagaa tccggtggag caggagggca cctatcctct tcctgaggcg
     1141 cagctcgaca gattcatcat gaaaattaat gtggaatatc ccagccagaa agaggaatct
     1201 cagattgtaa gaatgattat gaatgaggtc tctcttcctg aagtcaagaa ggttctcgag
     1261 ccgcaggaca ttctgaatct tcagtccgaa gcgagaaagg ttcatataga agaaaaaatt
     1321 atatcgtata taactgatat tacgcaggcg acaagacgcc cttccgagtt caggctggat
     1381 ctttccgaca tcattcaata cggagtttct ccccgtgcct ccatcgcaat ggcagtgctt
     1441 tccagggcga gggcattcat caagggaaga aatgctgtcc ttcctgagga cgtgaaagca
     1501 gttgccttcc ctgcactgcg tcacagactg attcttacat accatgcaga ggcggaaaac
     1561 atcaggcccg atgccgtaat cagtaaaatt ctggataatc tgaaagtccc atgatttcgg
     1621 aaaaggacct tctgcagaga gtgaggaatc tggagcttgt cggaagaaaa aatgcccatt
     1681 ctcttctgaa cggagagtat tccacaaaaa ttcccggaaa cggactgcag tttcacgaag
     1741 caaggaaata tgtatccggt gaatctatcc gtctcatcga ctggaatatt accgcaagac
     1801 agggtgagcc gtatgtgaag gtgcttctgg aagaaagaga aagagaaatt gtgatcgcac
     1861 ttgacttaag tcagtccatg cattccggtt ttcaggacag gacgaaactg gagtatgcag
     1921 tggaactcgc tgcctctgtt gctgtttctg ctgccgattc cagagacagg gtagggtgca
     1981 ttctttttac cgacaggact gaa
//
LOCUS       DAOQGJ010000700         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38771_length_2001_cov_6.612025, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000700 DAOQGJ010000000
VERSION     DAOQGJ010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38771_length_2001_cov_6.612025"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1076
                     /locus_tag="PK683_11200"
     CDS             258..1076
                     /locus_tag="PK683_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09060.1"
                     /translation="MFKKTQLINLAVASLFAAGTAVSAANVTVFVHGKSSSNHNGTGT
                     TDVNNYWGTSPNTVSGTKYFVGYDGTSDPRTYGAARAQTNLTTVMINVCKTNTCKIVC
                     HSAGCYATEYWLSNLGGTAASKGYSISGVTALAAASGGSELASALNAITFGYAGNAMD
                     KALIVSNARSSFNHNNTAGVTISHVPGSKGMIGASLILPGEDDYAVAFHSSCGYSTVG
                     GLSKCQSSITQSEGIWPFNSNKTYVQYIGHTRAPSVPVAGLYENHSELTDDGWR"
     gene            1240..>2001
                     /locus_tag="PK683_11205"
     CDS             1240..>2001
                     /locus_tag="PK683_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09061.1"
                     /translation="MFKKSQLINLAVASLFAAGTAVSAANVTVFVHGKSSSNHNGTGT
                     TDVNNYWGTSPNTVSGTKYFVGYDGTSDPRTYGAARAQTNLTTVMINVCKTNTCKIVC
                     HSAGCYATEYWLSNLGGTAASKGYSISGVTALAAASGGSELASALNGITFGYAGNAMD
                     KALIVSTARSSFNHNNTAGVTISHVPGYKGMAGASLILPGEDDYAVAYHSSCGYSTVG
                     GLSKCQSSITQSEGVWPFNSNVTYVQYTGHTRAPSV"
BASE COUNT          559 a          455 c          434 g          553 t
ORIGIN      
        1 tgcgagccgt taggcagccg ttaggcgtaa ggtaagcgaa tcatttgcat agtccagttt
       61 tgacttcagt gatctcattc tccctatcac tgcagtcgga gaataatctt tcattgactg
      121 tgcagtaaaa atctttccct tgaatgttta gaaaaaaatt atttaaaggg ataatttttg
      181 atttgcaatt attgttttcc gcgtcagatt caaatactgc cggtcaaacg gcaatcacat
      241 caaaaggaaa attcacaatg tttaaaaaaa cacaattgat aaatcttgct gtcgcttctc
      301 tctttgctgc cggaactgct gtttccgctg cgaatgtaac tgtgttcgta cacggaaaat
      361 caagctccaa tcacaacggg actggaacga cagatgtaaa caattactgg ggaacttcac
      421 ctaacacagt atccggaact aagtattttg tcggatatga cggaacaagt gatcctcgta
      481 catacggagc tgcacgtgct cagactaacc tcaccactgt tatgataaac gtatgcaaga
      541 caaatacttg caagatcgtt tgtcacagcg cgggatgtta tgcaactgaa tactggcttt
      601 ccaaccttgg gggaactgct gcaagcaagg gatattcgat ttccggagtc acagctctcg
      661 ctgctgcttc cggaggttct gaactcgctt ctgctctgaa tgcaattaca ttcggatatg
      721 caggaaacgc aatggacaag gctctgatcg taagcaatgc gagaagttct ttcaaccata
      781 acaacactgc tggtgttaca atcagccatg ttcctggttc aaagggaatg atcggtgctt
      841 ctctgattct gcctggggaa gatgactatg ctgtggcatt tcactcttca tgcggataca
      901 gtactgtagg cggtctgtcc aaatgccaaa gctccatcac tcagtcggaa ggcatctggc
      961 cgttcaattc aaacaaaacg tatgttcagt atatcgggca taccagagct ccttccgttc
     1021 cagtggcagg tctgtacgaa aatcacagtg aactcactga tgacggctgg agataagatc
     1081 cccggtaaac aacagtaatt cagttccatt acgagacggt tttttccgtc tcttttttta
     1141 tccgaattct caaaaaaata tttccacatt ttatctaatg gtttacatct ttcagcagta
     1201 tgatgtaatg gatctactaa atgaaaaagg aaattcacaa tgtttaaaaa atcacaattg
     1261 ataaatcttg ctgtcgcttc tctctttgct gccggaactg ctgtttccgc tgcgaatgta
     1321 actgtgttcg tacacggaaa atcaagctcc aatcacaacg ggactggaac gacagatgta
     1381 aacaattact ggggaacttc acctaacaca gtatccggaa ctaagtattt tgtcggatat
     1441 gacggaacaa gtgatcctcg tacatacgga gctgcacgtg ctcagactaa cctcaccact
     1501 gttatgataa acgtatgcaa gacaaatact tgcaagatcg tttgtcacag cgcgggatgt
     1561 tatgcaactg aatactggct ttccaacctt gggggaactg ctgcaagcaa gggatattcg
     1621 atttccggag tcacagctct cgctgctgct tccggaggtt ctgaactcgc ttctgctctg
     1681 aatggaatca cattcggata tgcaggaaac gcaatggaca aggctctgat cgtaagtact
     1741 gcaagaagct ctttcaacca taacaacact gctggtgtta caatcagcca tgtgccaggt
     1801 tataagggta tggcaggtgc ttctctgatt cttcccggtg aggacgacta tgctgtcgca
     1861 taccactctt cctgcggata cagtactgta ggcggtctgt ccaaatgcca aagctctatc
     1921 actcagtcgg aaggtgtatg gccgtttaac agcaatgtta cttacgttca gtacactgga
     1981 cacactagag ctccttccgt t
//
LOCUS       DAOQGJ010000701         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38841_length_1999_cov_2.958848, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000701 DAOQGJ010000000
VERSION     DAOQGJ010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38841_length_1999_cov_2.958848"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PK683_11210"
     CDS             <1..842
                     /locus_tag="PK683_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09062.1"
                     /translation="ETLMKRMSIFLLYVLAAQTLFCSHSDRKSIHTRQGLLALAAFQS
                     AAVNTVCTGTRYDDSPKMFNLSIDHEAWNGIANSTAGAFLFNSSAPKNLFEVYGASIS
                     GANGTSTNASYTWTTKKSAKVYSPIKGYVSRLELQSNEWWSQDYEIELKTNANSVYVF
                     ILDHFNSPLVSKGSLVTVGQELGTTGNLISGSASNPVMNTVYSAYELQVSKYSVGSNC
                     TDQSTTGLKSVCPTSLLDSSVSDSMKSKLLKLMSDWETLKGNSAIYNENTMIIPGCYS
                     ESF"
     gene            1005..1991
                     /locus_tag="PK683_11215"
     CDS             1005..1991
                     /locus_tag="PK683_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09063.1"
                     /translation="MKYDLAAGFAAMTVLIFSSGCSSLNTEERKAERILWKDVTASAY
                     HGKTMSQNKFAELKKEELKKFKSELEAVYRVPFKYRYTKYFNIAYRCSDSDADRIERN
                     LKIFFRDVYPKYYIYEPKFTMRIVYFGTKKEFIKHTNYDSYGYYMGVRDSENDTNRTL
                     YTYWNSGPGTLWHEMIHAFTDANTDMDVDSWFNEGMASFYEHGSTADGRFTEGYANWR
                     LPALQETIRAGRLPGLRKFFEKGLVDREYGYPYMRFLFCYLWIHDQMIPFVKAYIYEL
                     LPKYKGSELTGKSIETMERLLGKNISEIEKDMSGLALKLKKNEKLVKLKERK"
BASE COUNT          631 a          375 c          427 g          566 t
ORIGIN      
        1 aggagactct gatgaaacga atgtcaatct ttttattata tgtgcttgct gctcagactt
       61 tattttgcag tcattctgac agaaaaagca tccataccag acaggggctt ctggcacttg
      121 cagcttttca gtctgctgca gtaaatactg tctgcacagg aactcgatat gatgacagtc
      181 ctaaaatgtt caatttgagc atcgatcatg aggcctggaa cggcattgcc aactcaaccg
      241 caggtgcatt cctgttcaat tcttctgcac cgaagaatct cttcgaagtt tacggagcat
      301 ccatttcagg tgcaaatggc acaagcacaa atgcttccta tacctggaca acaaaaaagt
      361 ctgcaaaagt atattctcca atcaaggggt atgtcagccg tctggaactg cagtcaaatg
      421 aatggtggtc acaggattat gagattgaac tgaaaacaaa tgcaaattct gtttacgtat
      481 ttattctgga tcattttaat tcgcccttgg tttccaaagg aagcctagtc actgtcggtc
      541 aggagctcgg taccactgga aatctgattt ccgggagtgc atcaaatcct gttatgaata
      601 cagtatactc tgcatatgag cttcaagtca gtaagtattc tgtcggttca aactgcactg
      661 atcagtctac gactggtctg aaaagtgtat gtccgacttc tcttctcgat tccagtgtat
      721 ctgattctat gaaaagcaag cttctgaagc tgatgagcga ctgggaaact ctgaaaggaa
      781 attcagcaat ttataatgaa aatacaatga taataccagg atgttattct gaaagttttt
      841 gaatcaggtg cttgcggaca gcagaatgaa tatgcaggaa atatgagcaa aagctttttc
      901 acagacttcc ggcttatctg acttgctgta tttccgcaga acagatttcc ttgactcaat
      961 atttgcctat tgacagaaat tttcctttat aaatatatta tcttatgaaa tatgatttag
     1021 ctgcaggctt cgctgcaatg actgtcctga tcttctcttc agggtgctcc tcattgaata
     1081 ctgaagaaag gaaagcagaa aggatactgt ggaaagatgt gacagcatct gcttatcatg
     1141 gaaaaacaat gtctcagaac aaatttgccg agcttaagaa agaagagctg aagaaattca
     1201 aatccgaact ggaagcagtt tatcgggttc ctttcaaata cagatataca aaatatttca
     1261 atattgccta caggtgctct gattccgatg cagacagaat tgaaaggaat ctcaagatat
     1321 ttttccggga tgtctatcct aaatactata tctatgaacc gaaatttaca atgcggattg
     1381 tatacttcgg aactaaaaag gaatttataa agcatacaaa ttacgattca tacggttatt
     1441 atatgggtgt cagagattcg gagaatgata ctaacagaac actctataca tactggaatt
     1501 caggtccggg aactctctgg catgagatga tccatgcatt tacggatgcg aataccgata
     1561 tggatgtgga ttcctggttc aatgaaggaa tggcatcttt ttatgaacat ggaagcactg
     1621 cagacggaag attcacggaa ggttatgcaa actggagact tcctgcgctt caggaaacaa
     1681 tccgtgcggg cagacttccg gggcttcgga aattttttga aaaaggtctt gtggacagag
     1741 aatacggata cccgtatatg agattcctgt tctgctatct gtggatacat gatcagatga
     1801 ttccatttgt aaaggcatac atctatgaac ttcttcccaa atacaaggga agtgagctga
     1861 cagggaaatc catcgaaact atggaaaggc tcctgggaaa aaatatttca gaaatcgaga
     1921 aggatatgtc cggacttgct ttgaagctga agaaaaacga gaaacttgta aaattgaaag
     1981 agaggaaata acttacatt
//
LOCUS       DAOQGJ010000702         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38867_length_1998_cov_3.068451, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000702 DAOQGJ010000000
VERSION     DAOQGJ010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38867_length_1998_cov_3.068451"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK683_11220"
     CDS             <1..308
                     /locus_tag="PK683_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09064.1"
                     /translation="LLFIAFAGIFNCGDDSVASCKRSCTNQRNICFLALSDQNLTGGE
                     AVVCSLITETCSSSCYSRSSSSGTRTTSSSSSRSSGSSSSGSGSSGSGSGGHGGGGH"
     gene            complement(365..691)
                     /locus_tag="PK683_11225"
     CDS             complement(365..691)
                     /locus_tag="PK683_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH09065.1"
                     /translation="MEFTKKDHGAHKIFELKGSLDIYSASKMKKEINAIIDEDEIESL
                     ILDMSQVTHMDSSGIALIANLQKKMKTAGSKFALLKVTNDIMAVLRLSSLDNFFTIYG
                     SEADIK"
     gene            complement(706..1260)
                     /locus_tag="PK683_11230"
     CDS             complement(706..1260)
                     /locus_tag="PK683_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09066.1"
                     /translation="MSFDFNSLQDIFEIGENILKQISKPEYSEFLKTAMKELMQNSIK
                     ATLKRLHFEQKGLDMRTEYSAGMQSFQEFLNSARLKDLPSELQFSCQISFQKIGNTLF
                     IRVLNFGTLLPQEIRSIEAMLDRGRRNNSVADLLEDESRLKEGGGLGLSMIIVIGKSL
                     HFSGDFLSYKSDSGLTEFCLKIPV"
     gene            complement(1418..1801)
                     /locus_tag="PK683_11235"
     CDS             complement(1418..1801)
                     /locus_tag="PK683_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09067.1"
                     /translation="MAESKKERLKIEEEDFLILENMSGELAQILKERYDLKVKDESAW
                     PKRYSYSHSGLDLEFIFSSMGSFTVRPEISERYKKNPPPIFYISVGKYLDAFIWEDIN
                     FTEVPFDRIPESLEESLKNYAEYSA"
     gene            complement(1774..>1998)
                     /locus_tag="PK683_11240"
     CDS             complement(1774..>1998)
                     /locus_tag="PK683_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNH09068.1"
                     /translation="EKKIEEELGDLLFAVVNLSRFIKVNPEKALLSACDKFRKRFEFI
                     ELKAGESNQRLEEMSLSEMEELWQKAKKNV"
BASE COUNT          603 a          436 c          355 g          604 t
ORIGIN      
        1 tactgctttt tattgctttt gcaggaattt tcaactgtgg tgacgactct gttgcctcct
       61 gcaaaaggtc ctgcacaaac cagagaaata tctgcttcct tgctctgtca gaccaaaatc
      121 tgacaggagg agaggcagta gtctgctcac tgattactga aacctgcagc agttcctgct
      181 attccagaag cagttcaagc ggaacaagaa ccaccagctc atcaagcagt cgatccagtg
      241 gaagctccag cagtggaagt ggatcaagcg gaagcggatc aggagggcat ggcggcggcg
      301 gccattaaaa aatgaacggc ccccgaaatt ttcacgaacc agtcaacgac cagccgatgt
      361 atttttattt tatatctgcc tcagaaccgt atattgtaaa gaaattgtca agagaagaca
      421 gtctcaatac agccataata tcattagtaa cttttaaaag tgcaaacttg cttcccgctg
      481 ttttcatttt tttctgcagg ttggcaatca gagcaattcc ggacgaatcc atatgagtta
      541 cctgagacat atccagtatt aaagattcaa tttcatcttc atcgataatt gcattgattt
      601 cttttttcat tttagatgct gaataaatat ctagacttcc cttcagttcg aaaattttat
      661 gagcaccgtg atcttttttt gtaaactcca tatccactcc ttaatctata caggtatttt
      721 taagcaaaac tcggtcaatc ccgaatcaga tttataagat aaaaaatcac cggaaaaatg
      781 aagcgatttt cctataacta taatcatgct gagtcctaag cctccgcctt ccttgagtct
      841 gctttcatct tcgagcagat ctgcaacgga attgtttctt ctgcctctgt caagcatcgc
      901 ttctatgctt ctgatttcct gaggaagaag agtgccgaaa ttgaggaccc tgataaacag
      961 agtatttcca atcttctgaa aactgatctg gcaggaaaat tgaagctcag aaggaagatc
     1021 cttcagcctg gctgaattca gaaactcctg aaaactctgc atcccggccg aatattctgt
     1081 gcgcatatcc agtcctttct gctcaaaatg aagtctcttc aaagttgcct tgatggaatt
     1141 ctgcatcagc tctttcattg ctgttttcag aaattcagaa tattccggct tggaaatctg
     1201 cttcagaatg ttttctccta tttcaaagat atcctgaagg ctgttaaaat caaaactcaa
     1261 atattgtaca gaaaactgcc tttcagataa aaaatctgaa tatatatctt ccggaacaat
     1321 aagctgagaa ttcatgattg cggagtacaa atagcagaat gagcaattta gtcaacggtt
     1381 ttttttagat ataatgaaaa gaataatcct tcagcgacta tgcagaatat tctgcatagt
     1441 tttttaaaga ttcttccaag ctttcaggaa ttcggtcaaa cggaacctcc gtaaaattaa
     1501 tatcttccca tataaaagca tctaaatatt ttcccacact tatataaaaa atcgggggag
     1561 gatttttttt gtatctctcg cttatttcag gacggacagt aaacgaaccc atggaagaga
     1621 aaatgaattc aagatccaga ccggaatgac tgtaggaata acgcttgggc caggccgact
     1681 catctttcac cttaagatca tatctttcct ttaaaatctg agcaagctca ccggacatat
     1741 tctcaagaat aagaaaatct tcctcttcaa tcttcagacg ttcttttttg ctttctgcca
     1801 cagttcctcc atttcggaaa gactcatttc ttccagtctc tgattcgatt cgcctgcttt
     1861 cagttcaata aattcgaaac gttttctgaa tttatcacag gcagaaagaa gcgccttttc
     1921 cggattaacc ttaataaatc gggaaagatt tacaacagca aaaagcagat cccccagttc
     1981 ctcttcgatt tttttctc
//
LOCUS       DAOQGJ010000703         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38872_length_1998_cov_2.805455, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000703 DAOQGJ010000000
VERSION     DAOQGJ010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38872_length_1998_cov_2.805455"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1745)
                     /locus_tag="PK683_11245"
     CDS             complement(30..1745)
                     /locus_tag="PK683_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNH09069.1"
                     /translation="MSFGIPDYIVTAVFMAMTFGAAYFFSSKEKTLDSHFRSSGSLSW
                     FVSGTAMVATTFAADTPLAVTELVAKYGIAGNWIWWYMGISSVATVFFFAPLWKASGV
                     GTDIELVKLRYDSGSDILRIIKSLYLGLLMNIIILTWVNLAMLKICTVLFPSYPAWAI
                     VSFFCFAGFLYTGLSGLRGISYTDAFQFFFAIGACIVLAWFALADERIGGTAGLQAKL
                     PSEFFSIFPSDGKVSAFSSQEFIIFVTVVWWASWYPGAEPGGGGYIAQRILASKDEKS
                     SMFAALWFTIAHYFIRPWPWILTALASTVLFPNLSSADKGNGFVMVMKEVLPSGLLGL
                     LFSAFLAAYLSTVATHLNWGSSYLTSDLYKPYLNPGKEDSHYLKFSLLVEFISMAASL
                     TAAFFYVDSISGIWKFLLECGAGVGFALIARWFIPRINAWAELAGFVSPVIFYTLNKL
                     FFHIPAPLSIVFVSLSVSLTVVLTAVLTVPVNAGVLKHFYEKVKPPGYFWKKWAQKHG
                     VIEEEPFLKPLPAFFCSISALVLVYSLLCAAGYFIFGKYPELAVSSVTALGSGMFLYF
                     RLKSA"
     gene            complement(1742..>1998)
                     /locus_tag="PK683_11250"
     CDS             complement(1742..>1998)
                     /locus_tag="PK683_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNH09070.1"
                     /translation="ILLTSMDRDGTKDGFDINLLKLISQNVRIPVIASGGAGNAEHFT
                     EAIIRGEADAVLAASIFHYGEFSIRDIKASMKEMGIPVRL"
BASE COUNT          663 a          472 c          429 g          434 t
ORIGIN      
        1 cgactggatc cggctggacg aataaggact caggcagatt tcaatctgaa atacagaaac
       61 attcctgaac cgagagcagt cacagaggaa acggcaagtt caggatactt tccaaatata
      121 aaatacccgg ctgcacatag aagggagtaa acaagaacaa gcgcagaaat actgcagaaa
      181 aaagcaggca gaggtttcag aaatggttcc tcttcaatga ctccgtgctt ctgtgcccat
      241 tttttccaga aatagccggg aggcttgact ttctcataaa aatgcttcag caccccagca
      301 ttcacaggaa cagtaagaac tgctgtcaga acaactgtca gactcacaga aagacttaca
      361 aagacaatgg ataagggtgc agggatatga aaaaataatt tatttaaagt ataaaatatc
      421 acgggggaga cgaaaccggc aagctcagcc caggcattta ttcgaggaat aaaccatctt
      481 gctataagcg caaatcctac cccagccccg cattccagaa gaaatttcca aatcccgctg
      541 atcgaatcca cataaaaaaa agcagcagta agcgatgctg ccattgaaat aaattcgaca
      601 agaagcgaaa acttcagata atgagaatct tcctttcctg gattcagata gggcttgtaa
      661 agatcacttg tcagatagga tgatccccag ttgagatgcg tagcaacggt ggaaagataa
      721 gcagccagaa aggcagaaaa aagaagtccc agcaggccgg aaggaagcac ctccttcatt
      781 accatgacaa atccattgcc tttgtccgca ctgctcagat ttggaaaaag tactgtactg
      841 gcaagtgccg tcagtatcca tggccaaggc cggataaaat aatgcgcaat ggtaaaccaa
      901 agtgcggcaa acatcgaaga cttttcatcc tttgatgcca gtattctctg tgcaatatat
      961 ccgccgccgc caggctctgc acccggatac cagcttgccc accagacgac ggtcacaaaa
     1021 atgatgaatt cctgactgga aaaggcagac acttttccat ctgaaggaaa gatggaaaaa
     1081 aattctgaag gcagtttcgc ctgaagacct gctgtccctc cgattctttc atccgccaat
     1141 gcaaaccagg caagtacaat gcaggccccg atcgcaaaga aaaactgaaa tgcatcagta
     1201 taagaaattc cgcgcagtcc agacagaccg gtatacagaa aaccggcaaa gcagaaaaag
     1261 cttacaatcg cccaggcagg ataggaagga aagaggactg tacagatttt cagcatcgcc
     1321 aaattcaccc aggtcaggat gatgatattc atcaagagac ccaagtataa tgattttata
     1381 attcttaaaa tatcagatcc ggaatcatag cggagtttta caagttcaat atctgtaccg
     1441 acaccgctcg ccttccatag aggcgcaaaa aaaaacacag tggcaacaga actgattccc
     1501 atgtaccacc agatccagtt accggcaatt ccgtatttcg caacaagctc agtgactgca
     1561 agaggagtgt cggctgcaaa agtggtagcc accattgcag taccggaaac aaaccaggaa
     1621 agacttccgg aagaccggaa atgcgaatcc agagtctttt cctttgatga aaagaaataa
     1681 gcggctccga aagtcatcgc cataaaaacg gccgtgacaa tataatccgg aataccgaaa
     1741 ctcatagtct gacaggaatt cccatttctt tcatgcttgc ctttatgtcc cggatggaaa
     1801 attctccata atgaaaaatt gatgcggcaa gcactgcatc tgcttcacct ctgataattg
     1861 cttcagtaaa atgttctgca ttccccgcgc cgccgctggc gatgaccgga attctgacat
     1921 tttgagagat cagcttcaga agattgatgt caaatccgtc tttcgttcca tctctgtcca
     1981 tggaggtcag caggattt
//
LOCUS       DAOQGJ010000704         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38896_length_1997_cov_3.665294, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000704 DAOQGJ010000000
VERSION     DAOQGJ010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38896_length_1997_cov_3.665294"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PK683_11255"
     CDS             complement(<1..1060)
                     /locus_tag="PK683_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09071.1"
                     /translation="MLPSKVCCFFVPEQFSFYPILLKKYLASKKLKYKIKKISPKEKV
                     NTLMKTVKESIILLLLIVHCTKDSSISRETAYLFYRGAMVSVSGKTMKGIISGGLVNV
                     YAVNAKGICNKDRSLGSALTDSEGNYTVKFQRTGSPVCIVTSPSSNSSVYDEVSKKTL
                     SWTGSSSMTVIVKEPAVRSKSGINMTPLSRFAAARFQSIAKDNSDVSYLNAQLTYSNK
                     QIITQFALNTAFRRNSDGSRNLSRASAVDMNTVPDYFDDGIDLDDNTNSTAKYMKALF
                     GAFSQIANSTKDSTSVSSDDIEQVLTAYEQDLADGSADGTDKNGTALTISGQSGTSSL
                     GINPLTSTLQTALSSCAVS"
     gene            complement(1255..>1997)
                     /locus_tag="PK683_11260"
     CDS             complement(1255..>1997)
                     /locus_tag="PK683_11260"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH09072.1"
                     /translation="DLLYEVVMSVKLFINKMPLAALLFFALLSVELGSQNTAESGQGT
                     VLYVNTKSGLILRKEADKKSQKLDLMPYGQKVISEQSFIPAGSADSAGGWVNVQYSGK
                     EGYAAGEFLSFIDPKKAVSSHWKCRTLYNYNPGKETYTGELVLFKDSTYLTVTNTEGR
                     SISDKGTYSQTGNRISFKSKMDQTPEELIIIKKYLIESKDKKLLDKDPNLISRLEKIW
                     KSMLDGDEKDAESEESKKMMMNLCSPMK"
BASE COUNT          549 a          418 c          392 g          638 t
ORIGIN      
        1 ctgataccgc acaactggaa agtgcagttt gaagagtaga agtcagcgga ttgatcccga
       61 gtgaagatgt gccagactgt ccggatatag tcaaagcagt tccattctta tcggttccat
      121 cagcagatcc gtcggcgaga tcctgttcgt atgcagtcag cacctgctca atatcatccg
      181 aagaaacgga agtgctgtct tttgtactgt ttgcaatctg agaaaatgct ccaaataatg
      241 ctttcatata tttcgcagta gaatttgtat tgtcatccag atctatcccg tcatcgaaat
      301 aatctggaac agtattcata tcaacagctg atgctctgct cagattcctt gatccgtctg
      361 aatttcttct gaatgccgtg ttcagtgcaa actgagtgat gatctgttta ttggaatacg
      421 tgagctgtgc attgagatat gagacatctg aattgtcttt tgcgatagat tgaaatcttg
      481 cggcagcaaa acggctgaga ggagtcatat tgattcctga ctttgatctg actgcaggtt
      541 ccttcacgat tacagtcatt gaagatgaac cagtccagct cagtgttttt ttcgaaacct
      601 cgtcatacac tgaagaattt gaggaaggcg aggtgacaat gcagacaggg gaaccggttc
      661 tttgaaactt gactgtataa tttccttcag agtcagtgag tgctgaacct aaggaacggt
      721 ccttattgca gatccccttc gcatttactg catatacatt gaccagtcct ccggatatta
      781 tacccttcat tgtctttcct gaaacggaaa ccatagcccc tctgtaaaac agataagcag
      841 tttctctgga tattgaactg tcttttgtac agtgaacaat aagaagaagc aggataatag
      901 actcttttac agttttcatc aatgtattta ccttttcttt tggactgatc tttttgattt
      961 tatacttcag ttttttggaa gcaagatatt tttttaagag aatgggataa aaagaaaatt
     1021 gttcaggtac aaaaaaacag cataccttgg acggaagcat agttgaactg gtattccgtc
     1081 agatacgaca gatcaatata ggggaaatat ctgatattat tctgaatttt gcttttttta
     1141 tgaaaacatt ggagcggtcc cctccgcaga tccaggaagg cattctcaac tttctgctgc
     1201 agaaaggaat tgaaactggt tttcgctttt acacagcaag tataaaaagc agcttcattt
     1261 catcggagaa cacagattca tcatcatttt tttactctcc tcggattcag cgtctttctc
     1321 atctccgtcc agcatggatt tccaaatttt ttccagtctg gaaatcagat tcggatcctt
     1381 gtccagaagt tttttatctt ttgactcgat cagatatttt ttaatgataa tcagttcttc
     1441 tggagtctga tccattttgc ttttaaagga gattctattt cctgtctgac tgtaagttcc
     1501 cttgtctgaa atacttctcc cctctgtatt cgtcactgtc agataagtac tgtccttgaa
     1561 aagtacgagt tctcccgtat atgtttcctt gccgggattg taattataaa gtgtcctgca
     1621 tttccaatga gaagagacgg cctttttcgg atcgatgaaa gaaagaaatt ctcccgcagc
     1681 ataaccctct ttgccgctgt actgtacatt cacccatccg cccgcactgt ctgcactgcc
     1741 agccgggata aaactctgct cagaaatcac tttctgtcca taaggcatca gatcgagttt
     1801 ctgagatttt ttatctgctt cctttcgaag tatcagtcct gattttgtgt tcacatacag
     1861 aactgtaccc tgtccactct cagcagtatt ctgagaaccc agttcgacgc ttagtagagc
     1921 aaagaataaa agagctgcca atggcatttt atttatgaat aattttacag acataacaac
     1981 ctcataaagg agatctg
//
LOCUS       DAOQGJ010000705         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_38994_length_1994_cov_3.897370, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000705 DAOQGJ010000000
VERSION     DAOQGJ010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38994_length_1994_cov_3.897370"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..422
                     /locus_tag="PK683_11265"
     CDS             105..422
                     /locus_tag="PK683_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNH09073.1"
                     /translation="MDQFQNVQVIKKANVYFEGKVTSRTVVFSDGSRKTLGIMLPGEY
                     EFGTSEKEIMEILSGKLQVLLPGSSEWKEFVGDTVFEVPADAKFKLKVQEITDYCCSY
                     LGK"
     gene            435..971
                     /locus_tag="PK683_11270"
     CDS             435..971
                     /locus_tag="PK683_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH09074.1"
                     /translation="MPPLATELQSSSDILNLWTGFRKEVLECLKKTPADKFSQRPESG
                     WSISEIGEHLYLTQWSFSRSVPITVGGKLGSDTDAQDQLNYREMRAGLTKPRGFKNPE
                     SVSPLSNYTLEQLLPLLDKSHKKLEEVLAKYSKADLQKRGYEHPAFGLLNMFNYSWVL
                     ALHEGAHLFALKQKTSGY"
     gene            1075..1632
                     /locus_tag="PK683_11275"
     CDS             1075..1632
                     /locus_tag="PK683_11275"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001974205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNH09075.1"
                     /translation="MKYFVSYPGSYNAVNWFLASINHASFGHLLSNLMFLFFLGRSVE
                     YKVGKGKWLLFYSMAALVSGLADSMIRGAMGDRVPAVGASGAIAGIAAVSALLCPYTM
                     PAAGKKIPFPVFIVSWLAVYYDFTNLFSRDNVAHWAHLAGFFSVFLTAYMLSEEDRHN
                     LRMGFMFNLVFFSLTIILLYFVENR"
     gene            complement(1634..>1994)
                     /locus_tag="PK683_11280"
     CDS             complement(1634..>1994)
                     /locus_tag="PK683_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09076.1"
                     /translation="GRVVIVHGCKDGKDREFEGTYRIYRIGPDGLLKSKFSFAGSAFD
                     SLHSSYYYVDTDGKREKIEDRESDKVHPQGLWTLPHDKKGETIIDFILDTKQTDPNSY
                     RKEENVRWQKEIDSCAK"
BASE COUNT          543 a          412 c          431 g          608 t
ORIGIN      
        1 tcgttccaaa gaaactattt gaacaaacaa cacaaatgca aagatgaaga aaactgcagg
       61 atcatatatt ttaatatggg ttccagcaga atctgaggga aacagtggat caatttcaaa
      121 acgttcaagt tatcaagaaa gcaaatgtgt attttgaagg aaaagtgacc agcagaactg
      181 tggtcttttc ggatggaagc agaaagactc tagggattat gcttccgggt gaatacgaat
      241 ttggaacatc tgaaaaagaa attatggaaa tcctttccgg aaaacttcag gtactgctcc
      301 ccggttcttc tgaatggaaa gaatttgtcg gggatactgt atttgaggtt cctgcagatg
      361 caaaattcaa actgaaagta caggaaatta cagactattg ctgttcttat cttggaaaat
      421 aattggagga ctttatgccg cctttggcca ctgaactgca atcttcttcg gatattctga
      481 atttatggac aggtttcaga aaagaagttc tggaatgtct gaaaaagaca cccgcagata
      541 aattcagtca aagaccggaa tcaggctggt ctatttctga aatcggggaa catctgtatc
      601 tgactcagtg gagtttttcc agatcagtgc cgatcactgt gggcggaaaa ctcggctcgg
      661 acactgatgc tcaggatcag ctgaactaca gagagatgag agctgggctc acaaaaccaa
      721 gaggtttcaa aaatcctgaa tctgtgtcgc ctttgagcaa ttataccttg gagcagctcc
      781 ttcctcttct ggataagtcc cataaaaaac tggaagaggt gcttgccaaa tattccaagg
      841 cagaccttca gaaaagaggt tatgagcatc ctgcattcgg tctgctgaat atgttcaatt
      901 acagctgggt gcttgctttg catgaagggg cacatctgtt cgcactgaag cagaaaacat
      961 cagggtactg atcctgtgaa aattctactg gaatttccgc tgacactgag tgtctgtgta
     1021 tttctatttt tttcattttt catcatacaa ctcgcagttc ctcagcagat acatatgaaa
     1081 tattttgtat cctatcccgg aagctataat gcagtcaatt ggtttcttgc gtccatcaat
     1141 catgcatcat tcgggcatct tctctccaat ctgatgttcc tgtttttttt gggaagatca
     1201 gtggagtaca aagtcggaaa agggaaatgg ctgctttttt attcaatggc agcccttgtt
     1261 tccggtctag cagattcaat gatcaggggt gcaatggggg acagagttcc tgcagtcggt
     1321 gccagcggcg caattgcggg aatagctgcc gtttctgcct tgctctgtcc ttatacaatg
     1381 cctgcagcag gaaaaaaaat cccttttccc gtttttattg tttcttggct tgctgtttac
     1441 tatgatttta caaatctttt ttccagagac aatgttgctc actgggcaca tctcgccgga
     1501 tttttttctg tttttcttac cgcatatatg ctgagcgaag aagacagaca caatctcaga
     1561 atgggcttta tgttcaacct tgtattcttc tcactgacta tcatccttct ttattttgta
     1621 gaaaacagat agtctacttg gcacaggaat caatttcttt ctgccagcgg acgttttcct
     1681 cttttctgta actgttcgga tcagtctgtt ttgtatcgag tatgaaatcg atgatggtct
     1741 ctcccttctt atcatgaggc agagtccaaa gaccctgagg atgcactttg tctgattctc
     1801 tgtcttctat cttttctctt ttcccgtccg tatcaacgta atagtaactg gaatgaagtg
     1861 agtcaaacgc agagccggca aaagagaatt tagatttcag cagtccgtca ggtccaatcc
     1921 tgtatattcg gtaagtgcct tcaaattcgc gatctttgcc atccttgcat ccgtgtacaa
     1981 tgacgactct gcct
//
LOCUS       DAOQGJ010000706         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39019_length_1994_cov_2.369263, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000706 DAOQGJ010000000
VERSION     DAOQGJ010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39019_length_1994_cov_2.369263"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PK683_11285"
     CDS             <1..1089
                     /locus_tag="PK683_11285"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09077.1"
                     /translation="LSSAFKDNSCLYKGVITGILRVSRESIFSGLNNLSVYSILDRPF
                     SDKFGFTDEELKQIITDLEQPVSYHQIKEWYNGYTFGNRTEIYNPWSVLNYIIGWEDG
                     FKPYWANTSSNDLILDRISRKKDSEMRESVERLLNGETVERELYENFVFSELDSSPDL
                     IWTLLTFSGYLTVQDSRGNNLYKLRIPNYEIKLIFRQMILGWLNAEISIMRNFLQETA
                     DCLVNNQIDKFAKGFRKIIGDTFSYFDTDGEPEKVYHAYTLGMLAVLCDDYVIRSNRE
                     SGNGRYDILMIPHDKSKNGIVMEIKQIEKKKRESAKKFNERIQKELDSDLLQIEENEY
                     YKELIDHRVSNIIKLPIVFAGKEAFFKT"
     gene            complement(1093..1491)
                     /locus_tag="PK683_11290"
     CDS             complement(1093..1491)
                     /locus_tag="PK683_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09078.1"
                     /translation="MPLQFEGSLGLYYGLRLGFNAYEALDFIAGIFGIDVLKDDTLEP
                     MTEKDRREMLSEIHLQIRGVPLPAQDIGEICSAEKLCLNSVCLDGKCHEKGSEDRIPW
                     KCNEKGCGVSGASCKLDTECKGICVNGKCQ"
BASE COUNT          611 a          422 c          394 g          567 t
ORIGIN      
        1 ttaagcagtg cattcaagga taattcctgt ctatacaagg gagtaattac ggggatactc
       61 cgagtatccc gggagagcat tttttccggt ctgaacaatc tgagtgtgta cagcatcctt
      121 gatcgtcctt tctccgataa attcggtttc acggatgagg agctgaaaca gatcataact
      181 gacctggaac agccggtgtc ttatcatcag atcaaggaat ggtacaacgg ctatactttt
      241 gggaaccgga cggaaatcta caatccctgg tctgtgctga attatatcat cggctgggag
      301 gacggtttca agccctattg ggcaaatacg agttccaatg atctcattct ggacagaatc
      361 agccgaaaaa aagattccga gatgagggaa tctgtcgaaa gactgctcaa cggggaaaca
      421 gtggaaagag aactgtatga aaactttgta ttttctgaac tggattcaag tcccgatctt
      481 atttggacac ttctgacttt cagcggttat ctgactgtgc aggacagcag agggaataat
      541 ctgtataaac tgagaattcc taattatgaa ataaagctta ttttccggca gatgattctg
      601 ggctggctga atgcagagat cagcattatg cgaaattttc tgcaggaaac tgccgactgt
      661 cttgtgaaca atcagatcga taaattcgca aaaggattcc gaaaaatcat cggagataca
      721 ttcagttatt ttgatacaga cggagaaccg gaaaaggtct accatgccta tactctaggg
      781 atgctcgctg tcctctgtga cgactatgtc atccgttcca atcgggaaag cgggaatgga
      841 agatatgata ttttaatgat tcctcatgat aagtcaaaaa acggaatcgt gatggaaatc
      901 aaacagatcg aaaagaaaaa aagagaatct gccaagaaat ttaatgaaag aattcagaag
      961 gaactcgact cggatcttct gcagatagaa gaaaacgaat attataaaga actcatcgat
     1021 cacagagttt ccaatataat caaactccct atagtatttg caggaaagga agcatttttt
     1081 aaaacttaat cattactgac attttccgtt gacgcagata cccttgcatt ctgtatccaa
     1141 tttgcaggat gctcctgata ctccgcatcc cttttcattg catttccaag ggattctgtc
     1201 ttcagaacct ttttcgtggc attttccatc caggcataca ctgttcagac agagtttttc
     1261 cgcagaacaa atttcaccga tatcctgagc aggaagagga actcccctga tctgcagatg
     1321 gatttcagac agcatttctc ttctgtcttt ttcagtcatt ggttctagtg tgtcatcctt
     1381 cagaacatct attccgaata tcccagcaat aaaatccagt gcttcgtaag cattaaatcc
     1441 cagacgcagg ccatagtaca atcctaatga tccttcaaac tgtaatggca tctcgcattt
     1501 tctgcccagt ttagaaagtc ggcagaattc ctggtcatac tcgcagtaca tggacgaaaa
     1561 agatacatcc ggaaatataa ataaagttac tgctgagtat ggcttagttt tactccttat
     1621 catactagca tatattggga cattttctat actattatta tgtataatac tagttccata
     1681 tgaaaccaaa atctttcttg taacacgaat aggatcacca gacctattta ctctgccctt
     1741 tgttaaatgt cctattgatg gaatctctcc gctatatccc gttttgtatt cacctaagct
     1801 gcctcccctg atcccataac cctcaccttg acttccatat tgcagtcctg ttccaaaaca
     1861 ccagataaag aatgacccgc ctgctacatt cttttccact ccgactgtaa acatatctcg
     1921 gaaatctaag gctcggtact ggagataatt ggagacagac tggcagtggg ataagaataa
     1981 tagaaatata atga
//
LOCUS       DAOQGJ010000707         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39061_length_1992_cov_4.215281, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000707 DAOQGJ010000000
VERSION     DAOQGJ010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39061_length_1992_cov_4.215281"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..754)
                     /locus_tag="PK683_11295"
     CDS             complement(275..754)
                     /locus_tag="PK683_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09079.1"
                     /translation="MKFDTGSTYTGGFVKGQPEGEGEYVEIDPKTKAKTVYTGEMKNG
                     QPNGIGKTVFPDGAVFEGMYTDNIPNGKGRFFLANGDKYSGEFKDGKYYGKGIYIFAD
                     GRRYEGNFKDDFFSGKGIMYYPDGQVYIGQWKKNKRSGKGMMLKKMYSGTWKDDVKQ"
     gene            complement(893..1954)
                     /locus_tag="PK683_11300"
     CDS             complement(893..1954)
                     /locus_tag="PK683_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09080.1"
                     /translation="MSKKRTAFFALLFIAVSAYAVSPDEENLNILIQENKAHFQFINV
                     AISNMRSPKLESPADKNTPPAKEDDDSPDKKMDNPYYNDFLTANLKDFEGTVLHFKGD
                     YKNAHAPLKEAQIKLKEIYESALERHTEFTRVLLIYATHKIIKTNDISAKYLLKQAYK
                     ELKLAENCYTMGWNNAPYQYRNKIALYESGIIASRKARRFGILALIEQKIPKDQKKIY
                     KKEKFNEFKDSEYDGSVNDYEYIKKTLRNNIENKWVEGKISANYKFERPDTATNFTYI
                     PNKHQPIDLMEMLDDVYGIITYNRVSVKEETDKFVRKEITKEELEPSVPVKKEDPKPA
                     VPNNSAAPSKTPAPAPVKN"
BASE COUNT          492 a          406 c          390 g          704 t
ORIGIN      
        1 agggtgccca tatcagactt atatgtgagc ctgcataagg aagtgacagg cctattctcc
       61 cgaatacata gtaggacaat gcaataagaa ctgttttcag cataaaagga acagtgctgt
      121 atccggtcag atgttttttt aaattctgat aactcattca gtgcaatgga gaattgaacc
      181 gaaggaaggt caagctaatt cgatatataa gctttataca aagttcactg gagaaaatgt
      241 ctaaacacaa tactgctgca gatattttca gtttctactg tttgacatcg tccttccatg
      301 tacctgaata catttttttc agcatcattc cttttccgct tctcttgttt ttcttccatt
      361 gaccgatata aacctgcccg tccggatagt acataatccc tttcccgctg aaaaaatcat
      421 ctttgaagtt tccctcatat cttcttccgt cagcgaaaat gtaaattcct ttcccgtaat
      481 attttccatc cttgaattca cccgagtatt tgtcgccgtt tgccagaaaa aatcttcctt
      541 ttccattagg tatattatct gtatacatac cttcaaatac cgcgccgtcc ggaaacactg
      601 tcttgccaat tccgttgggc tgaccgtttt tcatttctcc agtgtagaca gttttagcct
      661 ttgtcttggg atcgatttcc acatactctc cctcaccttc aggctgtcct ttcacgaatc
      721 ctcctgtata agtgctccca gtgtcaaatt tcaaggtacc agtaccgttt tgacagtcgc
      781 cctggatgca ggatgaattg gaatatgagg aagaagcagg tataagaaaa aacactgcag
      841 tgaagactgc agtgttcaat gaattaattt tcaagtgtta ctctcggttt tatcagtttt
      901 ttacaggagc cggtgcaggt gttttggatg gggctgcaga attgttcgga actgcaggtt
      961 taggatcttc ctttttaacc ggaacagaag gttcaagttc ttcctttgtg atttcttttc
     1021 tgacgaattt gtcagtctct tctttgacgg acactctgtt gtaggtaatg attccataca
     1081 catcgtcaag catttccatc agatcgatcg gctggtgttt gttcgggatg tatgtgaaat
     1141 ttgtagctgt gtctggtctt tcgaatttgt agtttgcaga aattttgcct tccacccatt
     1201 tgttttcgat attgttacga agagttttct taatgtattc gtagtcattt acagatccgt
     1261 catattcaga atccttgaat tcattgaact tttccttctt gtaaatcttt ttctgatcct
     1321 ttgggatctt ctgttcaatg agtgccagta ttccgaatcg tcttgctttt cgggatgcaa
     1381 tgattccgct ttcatacagt gctattttgt ttctgtactg ataaggagca ttgttccagc
     1441 ccatagtgta acagttttcg gcaagtttca gttctttgta tgcctgtttc agaagatact
     1501 tggcactgat atcattggtt tttatgattt tgtgagtagc ataaatcaga aggactcttg
     1561 taaattcggt atgtctctcc agggcactct cataaatttc tttcagtttg atctgagctt
     1621 ccttgagagg ggcgtgtgca ttcttgtagt cgcccttgaa atgaagaact gttccctcaa
     1681 agtctttcag atttgctgta aggaaatcat tatagtacgg attatccatc tttttatcgg
     1741 gagaatcatc atcttctttt gcagggggag tgtttttatc tgccggactt tccagtttag
     1801 gacttctcat attgctgatt gcaacattga taaactgaaa atgagccttg ttttcctgga
     1861 taaggatatt cagattctct tcatccgggg agactgcata cgcagaaacc gcaatgaata
     1921 gtaatgcaaa aaatgctgtt ctttttttgg acatgtgtac cgcctgttct acttataaat
     1981 ttcggcgaat tc
//
LOCUS       DAOQGJ010000708         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39082_length_1992_cov_2.618998, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000708 DAOQGJ010000000
VERSION     DAOQGJ010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39082_length_1992_cov_2.618998"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PK683_11305"
     CDS             <1..259
                     /locus_tag="PK683_11305"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNH09081.1"
                     /translation="AESEGRTSLGDDIFIHGKEASAGCLAMGDDAIEDLFVLVGITGI
                     ENVTVFISPNRTFEKDNSVQYPSWTDELYENTYNAIKASLN"
     gene            452..1717
                     /locus_tag="PK683_11310"
     CDS             452..1717
                     /locus_tag="PK683_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH09082.1"
                     /translation="MKIIKMGMAVFFSLGILWAGDKENPKAVLSLEQNISISAGESKI
                     VDVKVNVPKGYGLVVGHSGKSLNMIANFAQTPGSGIQFDIENRPSGTKKGNNLLAIGN
                     VNFGVRLSELKGAKSGTVFKSDIVFNYQLCAMSDGKCLSPDGISQSVSVKVSKDKEHR
                     FAGIRSRSADAIKWAGTYENALKAAKSSGQNIYVIVTAPTWCGACKMMERETFEKSDV
                     QQVLNSDFVSLQVLDTSNDLDKFNIRGFPTSFVLDSSGKELTQMVGGVRAPTFLNFVA
                     SFRKAKKSDNTDIPVPPDAPDSPNTDIPVPPTLDDSTTSAKTISWEVKKNSAQYGGAD
                     WSGFLGTMFGTEEQCRKACSEVEECHAFFIVLRGYLDLAPTGPNKFWRHFRPGECVFL
                     KGKPWLGEAGQSDMHYKKINGKDAFEIGK"
BASE COUNT          630 a          330 c          453 g          579 t
ORIGIN      
        1 agctgagtcc gagggcagga catcccttgg tgatgatata tttattcacg gaaaagaagc
       61 ttctgcggga tgtcttgcaa tgggagatga tgcaattgag gatctgtttg tgcttgtagg
      121 cataacaggc attgagaacg ttacagtttt catttcaccg aaccgaactt ttgaaaagga
      181 taattcagtg caatacccgt cctggacaga tgaactttat gagaacactt ataatgcgat
      241 caaagcaagc ttgaattaat ctgactgttt aataaatcat gaatttgaat aaaaaaccca
      301 atattcagca gaaaaatatt tccttgagaa atttcgtaaa tgtctaatat tagaccttca
      361 gattagaatg aatgctgata aattttcttt tattgatcgg ttaataagtt gaatttgagg
      421 caggatgttg atgctgatac aggagaattt catgaagatt atcaaaatgg gcatggctgt
      481 atttttcagc ctaggaatac tctgggcagg ggataaagaa aaccccaaag ctgttttgag
      541 cttagagcag aatatttcaa tctctgcagg cgaatctaaa attgttgatg taaaagtcaa
      601 tgtaccgaag ggatatgggc tggtagtcgg ccattccggc aaaagtctga atatgattgc
      661 taattttgca cagactccgg ggtctggaat tcagtttgat attgaaaaca gaccgtctgg
      721 aaccaaaaag ggaaacaatc tgcttgccat tggaaatgta aattttggag tacgtctctc
      781 cgaattaaag ggagcaaaat ctggaactgt ttttaagtca gatatagttt ttaattatca
      841 gctttgtgca atgtccgatg gaaaatgcct ttcgcctgat ggaatttcgc aatcagtaag
      901 tgttaaagta tcaaaggata aggaacatag atttgccggt attagaagcc gttctgctga
      961 tgcaatcaaa tgggctggaa cctatgaaaa tgctcttaag gcagcaaagt cctctggtca
     1021 aaatatctat gtgattgtaa cagctcctac atggtgcggg gcctgcaaaa tgatggaaag
     1081 agagaccttt gaaaaatccg atgtccagca agttttgaac agtgatttcg tttctctgca
     1141 ggtgcttgat actagcaatg atcttgacaa gtttaatatt cggggatttc ccacatcttt
     1201 tgtcttggat tccagcggga aagagcttac tcagatggtt ggcggagtaa gagctcctac
     1261 attcttgaat tttgtagcaa gtttcaggaa agcaaagaag tctgacaata ccgacattcc
     1321 agttcctcca gatgcgccgg attcaccgaa tactgatatt ccagtgcctc ccacattgga
     1381 tgacagtaca acttctgcaa aaacgatttc ctgggaggtc aaaaaaaaca gtgctcagta
     1441 cggaggagct gactggtcag gttttctagg gacaatgttt ggaacggaag aacagtgcag
     1501 aaaagcatgt tcagaggtag aagaatgcca tgcttttttt attgttttaa gaggttattt
     1561 agatctggcc ccgactggac cgaacaaatt ctggaggcat tttcgaccgg gagagtgtgt
     1621 atttctaaaa ggaaaacctt ggctgggaga ggctgggcag tcagatatgc attataagaa
     1681 aataaacggc aaagatgcct ttgaaatcgg caaataaaat ctgatttcct ttaatgaacc
     1741 ggagattact gaagtcactt ttatgccgcc tccggttttt tcttttcttg agcaggatgg
     1801 aagatatttt tcagtattga aaatttgata tatgcagaat agatgggaat aaatgcgaaa
     1861 cgcaaaagca ataatatact tttataatta tatcattttg ctgtaaagat tccaattatc
     1921 acagtcagca ggctgacaag agaataagag tcgtccatag aattcttaat ctgaataaat
     1981 gagaaaggtt tt
//
LOCUS       DAOQGJ010000709         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39142_length_1990_cov_2.653747, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000709 DAOQGJ010000000
VERSION     DAOQGJ010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39142_length_1990_cov_2.653747"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK683_11315"
     CDS             <1..611
                     /locus_tag="PK683_11315"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNH09083.1"
                     /translation="FISSSKKFGEKAMAKGNFLTVQSGRYKGKKIPLPSAVKGNVNCT
                     PALLKKALFSILDSMHLSGEIDRTESVFADLFGGSGQMGLEAVSQGFGASVIFEIDSE
                     RQKEMKKYFEIYTGEISCFKRDAFRQYDMEEIMKYQYRIYFLDFPYSFWKKDSEKLHR
                     LCTDILSLPGKNFIFVQSPENPDWPEFTFRQAGNSFLLSSNL"
     gene            775..1566
                     /locus_tag="PK683_11320"
     CDS             775..1566
                     /locus_tag="PK683_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09084.1"
                     /translation="MRQFGSSLIFAITAAGAVFFGNCTGEKKAKTSIPAPLIQSLLNS
                     GNENEMLSTADSSFDRSFSNTADNSPVKSMLLKDMNSLSKETASGTKTVSETVACSAG
                     GKAVHTGSYDLTVNSITDQFNRDLSIQNFTMTVTFDSCSFHEGMKLVSGSVTQKQTGT
                     MKVVMQKTDSTVNISVSGRTMEVTGTEKAERNGGMRPAVSITMTRDIRESFELNRMKF
                     TVNSSSELTDPVPLYIKGSVIGKVSVDTPRGKRERDISRTFEKTF"
BASE COUNT          588 a          395 c          425 g          582 t
ORIGIN      
        1 agtttatcag ttccagtaaa aaattcggag aaaaggctat ggcaaaagga aattttctta
       61 cagttcagtc aggcagatac aagggcaaaa agattcctct cccttctgct gtgaagggaa
      121 atgtaaactg tactcctgct cttctgaaaa aagcactgtt ttccatcctg gattcaatgc
      181 atctttcagg agagatagac aggactgaat ctgtatttgc ggatcttttt ggcggctcag
      241 ggcagatggg gcttgaggca gtgagtcagg gtttcggggc ctcagtcatt tttgaaatag
      301 attctgaaag acagaaagaa atgaagaaat attttgaaat ctatacggga gaaatcagct
      361 gttttaaaag agatgcattc cgtcagtatg atatggaaga aatcatgaaa tatcagtata
      421 gaatatactt tctggatttt ccatattcat tctggaaaaa ggattctgaa aagcttcatc
      481 gtctgtgcac tgatattctt tctcttcccg gaaaaaactt catttttgtg cagtctcctg
      541 aaaatcctga ctggcctgag ttcacattcc gtcaggctgg gaacagtttt ctgctgagca
      601 gcaatctctg acaggtgatc tttcttctgt ttttcatctg atttttttct cagtatggaa
      661 ccttttcttt aaaaactgtg tctcactttt tggaattggg gatattaaac agagtgtggt
      721 ttgtgaaacg gtcatcagaa ccgaaaaact gcagtcagaa tttaggagac acttatgaga
      781 caattcggtt catctttgat atttgcaata actgcagcag gagcagtttt tttcggaaac
      841 tgcaccggtg aaaaaaaggc gaagacttcc attcctgcac ccttgattca atccctgctc
      901 aattcaggaa atgaaaatga aatgctgagc actgcagaca gttcattcga cagatccttt
      961 tccaatacag ccgacaattc tccagtaaaa tcaatgcttc tgaaggatat gaactctctg
     1021 tccaaagaga ctgcgtctgg aacaaagact gtctccgaaa cagtggcctg ttctgcagga
     1081 ggcaaggcag tccatacggg atcttatgat ctgactgtaa attcgatcac cgatcagttc
     1141 aacagggatc tgagcattca gaattttacg atgacagtca catttgacag ctgcagtttt
     1201 catgaaggaa tgaaactggt ttcaggaagt gtgactcaga agcagacagg cacaatgaaa
     1261 gtcgttatgc agaagacaga cagcacagtg aatatatctg tttcaggccg aactatggaa
     1321 gtgaccggca ctgaaaaagc tgagaggaac gggggaatgc gtcctgcggt cagcatcaca
     1381 atgaccagag atattaggga atcatttgaa ctgaaccgga tgaaatttac agtaaactct
     1441 tcctctgaac tcactgaccc tgttcctcta tacatcaaag gatctgtcat tggaaaggtc
     1501 agtgtagata ccccgagggg caaaagagaa agggacatca gccgcacttt tgagaaaact
     1561 ttttaactga cactcctgtt tccccttttc tcacttgctg aaccggcaca gtcggttcag
     1621 cttttttttt gtcatatttc cctaatcaga acagttatct atttaagcat ctgatccaga
     1681 tttcattcag ttcaggactt cgcttaattt atttcctcag gggaagttgg aaaatgtatt
     1741 ttactgagaa taaaaagatt ctgctgatca gaccgtttat tctgtttttg atacgaaaat
     1801 tctttctgtt ctgctgtgta tttttcacag gatgtatgtg ggaaagaccg gtaaaaatta
     1861 aaggcatatt tactcttcca aaacttgctg aacagctgat ttcaaattct gcaccggtca
     1921 atgtaaatca gtgcggttcc aaaatccctt ctgaaccgac agcggtgagc atcagttcca
     1981 ctgtatcacc
//
LOCUS       DAOQGJ010000710         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39164_length_1989_cov_2.996381, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000710 DAOQGJ010000000
VERSION     DAOQGJ010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39164_length_1989_cov_2.996381"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PK683_11325"
     CDS             <1..1416
                     /locus_tag="PK683_11325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH09085.1"
                     /translation="AAFAAETAAHIRSEISNRPEEFGTTAKKVQEVLYTDEISQFISE
                     NTKIGVYSKITDLIQQNGNIGPYGLSEDHELFRDTFKNFAESVVMPLAEHTHRNDETV
                     PEEILQGLKDMGCFGLCIPETYGGIQPSDKPDNISMLVVTEELSRGGLGIAGSLITRP
                     EIVSKAILKGGTDAQKEKWLNKLATGEVMAAVAVTEPNYGSDVAGISVTAVKVDGGWK
                     INGVKTWCTFAGYANVLLVLCRTESDPSLKHKGLSILLAEKPSTREHSFDYTQPGGGR
                     IEGKAIDTIGYRGMHSFEVSFQDYFVPDENLIGGEAGRGKGFYLQMEGFAGGRIQTAA
                     RANGVMQAAFEAGWRYAGERQVFKKPIYDYNLTKYKLARMAMIIQASRQYTNTVGKML
                     DEHKGQMEATLIKFYASKIAEWVAREAMQIHGGMGYAEEYVVSRLFLDARVFSIFEGA
                     EEVMALRVIAKGLLDAAFAAK"
     gene            1413..1874
                     /locus_tag="PK683_11330"
     CDS             1413..1874
                     /locus_tag="PK683_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09086.1"
                     /translation="MSSLLKKLNFKAQDRILVLGPPSEFEPFLSEFQEYLKIDLNPSP
                     KIKYDFIITFSVMKKDLKTTLKKTSGAASEDCLYWFAYPKKTSKKYKSDLTRDEGWQQ
                     MGDLGYEGVRMIAIDDDWSAMRFKKADKIKTMKRNEKLAMSSAGKKRTRQG"
BASE COUNT          638 a          414 c          478 g          459 t
ORIGIN      
        1 gctgcattcg cggcagaaac agcagctcat atcagatcgg aaatatccaa ccgacctgaa
       61 gaattcggca caactgcaaa aaaagttcag gaagttctct acactgacga aatcagtcag
      121 ttcatctcag aaaatacaaa gattggcgtt tattccaaaa tcactgatct gattcagcag
      181 aacggcaata tcggtcctta tggactttcg gaagaccatg agcttttccg tgatacattt
      241 aaaaactttg cagaaagtgt agtgatgcct ctcgcagaac acactcaccg aaatgacgaa
      301 actgtccctg aggaaatcct gcagggactc aaggatatgg gatgtttcgg actctgtatc
      361 ccggaaacat acggcggaat tcagccttcc gacaaaccgg acaatatcag tatgctggtt
      421 gtgacagagg aactctctcg cggaggactc ggtattgcag gaagtctgat caccagacct
      481 gagatcgttt ccaaggcaat cctgaaaggc ggaacagacg ctcagaaaga aaaatggctg
      541 aacaaattgg caacaggcga agtaatggca gccgttgcag tgactgagcc gaattatgga
      601 tctgatgtgg cagggatttc cgtgacagca gtgaaagtgg acggaggatg gaaaatcaac
      661 ggagtcaaga catggtgtac atttgcggga tatgcgaacg ttctgctggt actctgcaga
      721 acagaatccg atccttcact gaagcacaaa ggactctcca ttcttctggc agaaaaaccc
      781 agcacaagag aacacagctt tgactatacg cagcctggcg gaggaaggat tgaagggaag
      841 gcaattgata caatcggtta tcgcggtatg cattcctttg aggtatcttt ccaggactac
      901 ttcgttcctg atgaaaatct gattggagga gaagcaggaa gaggaaaagg attctatctg
      961 cagatggaag gttttgccgg aggaaggatc cagacagcag caagagccaa cggagtcatg
     1021 caggcggcct ttgaagcagg atggcgctac gcaggtgagc gtcaggtatt caaaaaacct
     1081 atctacgatt ataatctgac caagtataaa cttgcgcgta tggcaatgat cattcaggca
     1141 tccagacagt atacaaatac agtagggaaa atgctggatg agcacaaggg tcagatggaa
     1201 gctactttaa ttaaatttta tgcttccaaa attgcagaat gggttgcccg tgaagcaatg
     1261 cagatccacg gcgggatggg atatgcagaa gagtatgttg tatcaagact tttcctcgac
     1321 gcaagagtat tctctatttt tgaaggagcc gaagaagtaa tggctcttag agtaattgcc
     1381 aaaggacttc tggacgctgc tttcgctgcc aaatgagcag tctcctgaag aaactgaact
     1441 tcaaggccca ggacagaatt cttgtcctgg gtcctccctc tgaatttgag cctttccttt
     1501 ccgagtttca ggaatatctt aaaatcgacc tcaacccctc tcctaaaata aaatatgatt
     1561 tcattataac attttcagta atgaaaaagg atctgaaaac cacactcaaa aaaacatccg
     1621 gtgccgcatc tgaggactgt ctttactggt ttgcatatcc taaaaaaact tcaaaaaaat
     1681 acaagtctga tctgactcgt gatgaaggct ggcagcagat gggagatctg ggttatgaag
     1741 gagtcagaat gattgcaata gatgatgact ggtctgcaat gcgttttaaa aaagccgata
     1801 aaataaaaac aatgaaaaga aatgaaaagc tggctatgtc ctctgccgga aaaaaaagga
     1861 cgcgacaggg atgaaatccc cctcctccaa aaacagactc ccgttcgaca ataactactg
     1921 gaaaactatg tatgcagatg atgatgaatc agtcatcgac ggtctctaca atgcaaagga
     1981 acatgcgga
//
LOCUS       DAOQGJ010000711         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39236_length_1987_cov_2.866977, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000711 DAOQGJ010000000
VERSION     DAOQGJ010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39236_length_1987_cov_2.866977"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PK683_11335"
     CDS             <1..491
                     /locus_tag="PK683_11335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH09087.1"
                     /translation="ATLLKAVSLIPSKLKFKLFIVGEGELENDLKQLAEALKIEDKVV
                     FTGFRNDIPELYRFFHIFALTSKEEGLGTSVLDAMANSLPLAVTKGGGISEMVEEGQG
                     GFLAEIRDSVQIARILEEFIKDPALRKKYGAFNKKYVEKFHVKNTVAKTVMIYETLLG
                     PF"
     gene            complement(464..1261)
                     /locus_tag="PK683_11340"
     CDS             complement(464..1261)
                     /locus_tag="PK683_11340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH09088.1"
                     /translation="MNSAPIPISACVICLDEEDNIYDCLKSLWFAEEIIVLDSGSTDR
                     TVTIAKSFNNVKVFQREFDNYISQKNHAISLALNPTVLVLDADERISDSLSEEIRNRW
                     PLDAEISGMYIPRLTFYLGKWIRHGGWYPNHQLRLFKKEKGKFSGIILHEKVDLEGRT
                     EFFKNPILHYSYRNITDHLNFINRYSSLGAEEKFQKGKKSGVFQAVYRAFYKFLHMYF
                     GKLGLLDGRAGFIIAVLGAYYNFLKYIKLYELRNIRNENQNGPSRVS"
     gene            complement(1258..1929)
                     /locus_tag="PK683_11345"
     CDS             complement(1258..1929)
                     /locus_tag="PK683_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH09089.1"
                     /translation="MSETHPIDNLIEVVKDSENLVEIRTYRISQGLEEYIHHRLETML
                     TNSPRPTLLPMVYTVIKELSINACKANQKRVFFEEKNLDISKTSDYSKGIVLYRSSFS
                     ESMGAEYGQKCKEKGYYCLIKMEKTVNGVVLSVTNNTPIAKEEEASIREKLAKAMAYD
                     DLAQFYMDNADNTEGAGLGLALIIIMMKGEDLDPNYFRISIANGLTTARLEVPLTSEF
                     KSKRD"
BASE COUNT          563 a          393 c          408 g          623 t
ORIGIN      
        1 aggccacact tctgaaagct gtgtcactga ttccttccaa actgaagttc aaactgttta
       61 ttgttggaga aggagaactt gagaatgatc tgaagcagct ggctgaagct ttgaagattg
      121 aagacaaagt cgtatttacg ggtttcagaa atgacattcc tgaactgtac aggtttttcc
      181 atatatttgc tttgacttcc aaggaggaag ggcttggcac gtcggtactg gatgcaatgg
      241 caaattcact tccccttgca gtgacaaaag gcggaggaat ttctgaaatg gtggaagagg
      301 ggcagggcgg gtttctggca gagatcaggg actctgttca gattgcaaga atcctggaag
      361 aattcattaa agatcctgcc ctcagaaaga aatacggagc attcaataaa aaatacgtgg
      421 aaaaatttca tgtaaaaaat acggtcgcaa agactgtaat gatctacgaa actctgcttg
      481 gtccgttctg attttcattt cgaatattcc gcaattcata aagcttaata tactttagga
      541 agttataata agcacctaat actgcaatga tgaatcctgc gcgtccgtca agaagtccca
      601 gctttccaaa atacatatgc agaaatttat aaaatgctct gtaaactgcc tggaaaactc
      661 cgctcttttt tcccttttga aacttttctt cggcacccag actggagtat ctgttgatga
      721 aattcagatg atcggtgatg ttcctgtagg aataatgaag aattggattt ttaaaaaatt
      781 cagtcctgcc ttcgagatct actttttcat gcagaatgat tccggaaaat ttacccttct
      841 cttttttgaa cagacggagc tgatgattcg gataccatcc tccgtgacgt atccatttgc
      901 ccagatagaa tgtcagacga ggaatataca ttccggaaat ttccgcatcc aaaggccagc
      961 ggttccgtat ttcctcagaa agggagtctg atattctttc atctgcatcc agaaccagta
     1021 cggtaggatt taatgcgaga ctgatcgcgt gatttttctg acttatatag ttgtcaaact
     1081 ctctttggaa tacttttaca ttgttgaagg acttcgcgat agtaactgtt ctgtctgtag
     1141 agcctgagtc cagtactatg atttcctctg caaaccagag acttttcaga cagtcataga
     1201 tattgtcttc ttcgtccagg caaataacgc aggctgatat tggaatgggt gcggaattca
     1261 atcccttttg cttttaaatt cagaagtcag aggaacctct aatcttgctg tagtgagtcc
     1321 gtttgcgata gaaattctga aatagtttgg atccagatct tcgcctttca tcataatgat
     1381 aatcagtgca agacctaacc ctgcaccttc cgtattgtct gcgttgtcca tataaaactg
     1441 agcaagatca tcgtaagcca ttgccttagc cagtttttca cggatggaag cctcttcttc
     1501 tttcgcaata ggagtattgt ttgttacgct taatacaacc ccattgactg ttttttccat
     1561 tttaataaga cagtaatacc ctttttcttt gcacttctgt ccgtattcag cccccatact
     1621 ctcgctgaaa gaggatctgt agagaacaat ccctttggaa taatcagaag ttttggaaat
     1681 atccaagttt ttttcttcaa agaatactct tttttgattg gccttacagg cattgatgct
     1741 cagttctttg attacggtgt agaccatagg gagaagagtc ggtctgggac tgtttgtaag
     1801 cattgtctca agtctgtgat gtatgtattc ttccagtccc tgtgaaattc tgtatgtgcg
     1861 aatttctaca aggttttcag agtcttttac aacttcaatc agattgtcta ttggatgcgt
     1921 ctcagacatc tccgtaaaga tctgaggaaa gggcaaaaaa gtcaagcttt ttaaaaaaaa
     1981 attaaca
//
LOCUS       DAOQGJ010000712         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39319_length_1984_cov_3.556247, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000712 DAOQGJ010000000
VERSION     DAOQGJ010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39319_length_1984_cov_3.556247"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PK683_11350"
     CDS             complement(<1..724)
                     /locus_tag="PK683_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH09090.1"
                     /translation="MSLRNSSLERLKNETFDVIILGGGINGAVSAASLSAKGAKTALI
                     DERDFAGFTSQNSSNLAWGGIKYMESYEFGLVRKLCMSRNKLMKNYPSTVQEIRFYTT
                     ISKNFRYNRYFLYMGTLFYWLMGNFFTKPPRPLSRETVMKEEPLIDLTDSTGGFEYSD
                     CYLHDNDARFVFNFIRSAMNYGCAAANYVRSEGAKRENGIWTVQAEDRMTGKKFPIRG
                     KVLINSCGPFADEHNQKTGIRTS"
     gene            1003..1644
                     /locus_tag="PK683_11355"
     CDS             1003..1644
                     /locus_tag="PK683_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09091.1"
                     /translation="MRTFLKFFYISAVLFSAESCSTKKKASDKYLVLPFICCNYTPPI
                     FESSPTLIIQSGFENNTSIYNVSDNILGFQGTDRNFSSSNDWTKANADSSIGDFYLQL
                     GSPGTTSDRKAYITADPVNPANRALSFQIKNATEPDPLKGRTSMNIGENLKLAEIYYK
                     VKMFLPADVGYLKDYTFTSGNWLTLMEFWYSPDWTGNSAYPFRITLYVIKNSV"
BASE COUNT          576 a          447 c          369 g          592 t
ORIGIN      
        1 gacttgtcct gattccagtc ttctgattgt gttcatccgc aaaaggaccg caggaattga
       61 taagcacctt tcctcttatc ggaaatttct ttccagtcat cctgtcctct gcctgaacag
      121 tccatatccc gttctctctt ttggctcctt ccgatcgcac atagttggca gcagcgcatc
      181 cgtaattcat tgctgaacgg ataaaattaa atacgaagcg ggcatcattg tcatgaagat
      241 aacagtcgga atactcaaac cctcccgtgc tgtccgtgag gtcaatcaga ggctcttcct
      301 tcatgaccgt ctccctgctg agaggtctcg gaggttttgt aaagaaattt cccatcagcc
      361 agtagaaaag agttcccatg tacagaaaat atctgttgta acggaaattt tttgagattg
      421 tagtataaaa gcggatttcc tgaacagtgg atggataatt tttcatcagc ttgtttcggc
      481 tcatgcagag ttttctcacc agaccgaatt cataactttc catatatttg atcccgcccc
      541 aggccaggtt ggaagagttc tgactggtga acccggcaaa atctctttca tcaatgagtg
      601 cagtcttggc accttttgca gagagagagg cggcagaaac ggcaccgttg attcctcccc
      661 cgagaatgat cacatcgaat gtttcatttt tgagtctttc cagactgctg ttacgcaagg
      721 acatacatat catccaaaat taaattttcc gagttctgtc tgactgaatt tcattgagtc
      781 tctttaaaag agctctctgc atctcgcgga cactgagtat cagattcagc cggacatatt
      841 ttctcagata ctgaaaagat tcagaaaatc attgatttcg tataccattt tttatgaatc
      901 gacttaaact caccctcaat taatctgcag actgaggctg cttgagctga taattctgta
      961 aaatcaaatg cctctgacat aaaactgtat cggagcaaaa ccttgagaac atttctcaaa
     1021 tttttttata tctcagctgt cttattttca gccgaatcct gttccacaaa aaagaaagcc
     1081 tcagacaagt atcttgtcct gccgttcatc tgctgcaatt atactccgcc gatttttgaa
     1141 tcctctccta cattgatcat tcagtcggga ttcgaaaata atacatctat ttataatgtc
     1201 agtgacaata ttctcggctt tcaaggcaca gaccggaatt tttcgtcatc caatgactgg
     1261 accaaggcca atgccgattc aagcataggg gatttttatc ttcagcttgg atctcccggt
     1321 acaacttcgg acaggaaggc atacattact gcagatccgg tcaatcctgc caacagagct
     1381 ctttcttttc agatcaaaaa tgccacagaa cccgatcctc ttaaaggcag aacatccatg
     1441 aatatcggtg aaaacctgaa actcgcagaa atatattata aagtaaaaat gtttcttccg
     1501 gctgatgtcg gatatctgaa agactataca tttacttcag gaaactggct cactttaatg
     1561 gaattctggt acagccctga ctggacagga aattcagcat atccctttcg aattacatta
     1621 tatgtaatta aaaactccgt ttaaacagtc aggctttttc ttcgtatctg cactgtcctg
     1681 ccgaagcgcg gaattcatga atacagcccc tctcccagtc tgaaaacagc tgaagccgag
     1741 ataaagaata aattatttct gcagaatttt ttctcggaat ggtcctgctg aatgtgagcc
     1801 atattatata taattatttt tattaaacag gaaacagtca gacggaacgg ctgttaaata
     1861 tctcactcaa aaagcccggc agactggcaa taagttttca gtcttactgc ctttcaaaga
     1921 tgtgaacagt atagtttccc ccattttttg gacagtaaaa ttgaaaaaat aaggtggtaa
     1981 ataa
//
LOCUS       DAOQGJ010000713         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39339_length_1984_cov_2.452566, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000713 DAOQGJ010000000
VERSION     DAOQGJ010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39339_length_1984_cov_2.452566"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1627)
                     /locus_tag="PK683_11360"
     CDS             complement(89..1627)
                     /locus_tag="PK683_11360"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09092.1"
                     /translation="MRKLPIGIQTFSEIIEENYVYVDKTKWILDLLNAGKYLFFARPR
                     RFGKSLLISTLDSIFRGRKHLFSGLYIEDKIEWKEYPVIRLDLSTCQTDSPQNLEISL
                     KESLQVIAENYSVSLESPLVSGQLRELIRKLSARGRVVILIDEYDKPIVDRLTDLELA
                     GKMRDVLKNFYSVLKGSDEYIKSVFITGVSKFSQVSIFSGLNNITDISFFPEFSSLLG
                     YTQEELESCFSDRMSYLKNRTGMNEQEILNKMKFWYNGYSWDGTTRVYNPFSVLNFFN
                     TGEFHNFWFQTGTPTFLIRHIKAEKISVPSMENIEASPETFESSGIEETSFHSLLFQT
                     GYLTVREIRIRNNEKRYILDYPNEEVKRSFFVHILNSFLDGKKSVGQVSPIVQDMNSD
                     LRNNDLNSFILRLRSIFASIPYTLHIDSEAYYHSLFYMILKLIGCRIDLEVLSSEGRA
                     DGILEFSDRIYVIELKLDSPEKAIEQIHKKGYHNPYLSSGKKIYLLGIGYLNRTTEFI
                     LEEI"
     gene            complement(1804..>1984)
                     /locus_tag="PK683_11365"
     CDS             complement(1804..>1984)
                     /locus_tag="PK683_11365"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNH09093.1"
                     /translation="NIPKEKLVDVFAEHGNQVGASLPTALHFGISSGKIQRGNKVLFI
                     GTGAGLTIGGMVMIY"
BASE COUNT          576 a          411 c          390 g          607 t
ORIGIN      
        1 gatgaatatt ttaaagttca taaaggtctc ctgtgttttt caggacttca ttgtatctgc
       61 agacggaaaa tgtcaatcca aagcagaatt aaatttcttc cagaataaac tcagtcgttc
      121 tgttcaagta tccgatgccc agaagataaa tcttttttcc tgaactcagg taagggttat
      181 gataaccctt tttatgaatc tgttcgattg ccttttccgg gctgtccaat ttcagttcta
      241 tgacatagat tctgtccgaa aattcgagaa tcccatcggc acgtccctcg cttgaaagca
      301 cttccaggtc tattctgcat ccgatcagtt tcaggatcat atagaaaagg gaatgataat
      361 atgcttctga atcaatatga agagtatagg gaatggaagc aaaaatagaa cgcagtctga
      421 gaataaaact gttcagatca ttatttctaa gatccgagtt catatcctgc acaataggac
      481 tgacttgacc gaccgatttt tttccgtcca gaaaggaatt cagaatgtgc acgaaaaagg
      541 atctctttac ttcttcatta gggtagtcta agatatatct tttttcatta tttcttatcc
      601 gaatctcccg gactgtcaga taacctgtct ggaaaagaag agaatggaag cttgtctctt
      661 cgattccgga agattcaaat gtttccggag atgcttcaat gttttccatg gatgggacag
      721 agattttttc tgctttgata tgcctgatca gaaatgtcgg tgtcccagtc tgaaaccaga
      781 aattgtggaa ttcacctgta ttgaaaaaat ttaatacaga aaagggatta tagaccctgg
      841 tcgtcccgtc ccagctgtag ccgttgtacc aaaatttcat cttattcagg atttcctgtt
      901 cattcattcc tgtcctgttc ttcagataag acattctgtc cgaaaagcag gattcgagtt
      961 cctcctgtgt atatcccaaa agggaggaaa attcaggaaa aaaagatata tctgtaatat
     1021 tattcaatcc ggaaaaaatg gaaacctgtg aaaatttcga aactcctgtg atgaaaacag
     1081 atttgatgta ctcatctgaa cctttcagaa ctgaataaaa gtttttcaga acgtctcgca
     1141 tttttcctgc cagctccaga tcggtcagtc tgtcaacaat cggcttgtcg tattcatcaa
     1201 tcagtatgac gacccttcct ctcgcagaaa gttttcggat gagttctctg agctggccgg
     1261 aaaccagcgg ggattcaaga gaaactgaat aattttctgc aatgacctga agagactcct
     1321 tcagtgagat ttccaggttt tgcggagaat ccgtctggca ggtggaaaga tccaggcgga
     1381 tgacaggata ttctttccac tcgattttgt cttcaatata cagaccggaa aacagatgtt
     1441 tccttcctct gaaaatactg tcaagagtgc tgattaaaag agacttgccg aatcttctgg
     1501 gtcgggcaaa aaaaagatac ttccctgcat tcagaagatc cagtatccat ttcgtcttgt
     1561 ctacatagac atagttttct tcaatgattt ctgaaaatgt ctgaattcct attggaagct
     1621 ttctcacagt atgtccattc ccgatctgta aagattcatt gtcaattcta aaaggccgac
     1681 tgcagatctt ctgattgcag agacggtgga aactgcaggc ggtattcttt ggactttgga
     1741 ttctgatttt tccgcaatgg aaaaactgag ctttgtcctt ctctataaag aaatctaaaa
     1801 aactcagtaa atcatgacca tacccccgat tgtcagtcca gctcctgttc caatgaaaag
     1861 aaccttgttt cccctctgaa tttttccgga actgatcccg aaatgaagtg cagtgggaag
     1921 agatgcacct acctgatttc cgtgctctgc aaatacatcc accagttttt ccttagggat
     1981 gttc
//
LOCUS       DAOQGJ010000714         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39433_length_1981_cov_3.020768, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000714 DAOQGJ010000000
VERSION     DAOQGJ010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39433_length_1981_cov_3.020768"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1981
                     /locus_tag="PK683_11370"
     CDS             <1..>1981
                     /locus_tag="PK683_11370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH09094.1"
                     /translation="TEYGGEILGEEYFPIGIDKNDIQNLITDISKSGTDVFVPYFAGS
                     DQISLLNEFHSRGLKKKMAVVMGHYDEAMVSLLAPEVREGFYSSNTYFMTVNTAKNRE
                     VLKKLSAFPGVTGIWPDGNGILTNFGEGTYICMHAFAMGAERAGSVETEALTAALEKT
                     VIEAPQGTVRMDAATHHAEVHSYLSVCTKEGIFSIIKDFGPSAPVIPQRHRSLIPENK
                     PKETNFSSEILNKNLSFVSQSILRAIDVSVFTADENGVIIQANRGASETFGYSEEEFI
                     GMSVNMLLPPHLRSAHMRHMKEFIHDGKAEIRMGKRGEIAGYRKDGTFFPAEASVSKF
                     QAEGKWILVTTLIDVTDKKKSEDELKWKATHDPLTGLPNRTLIRDRLSAALQRTEKTG
                     MNIGLMFIDLDNFKLVNDSLGHSTGDDLLVRIADILTENVRPGDTVARLGGDEFVILC
                     EHMENKEELSGFAEILNNALRISFNYEGKEVSTSASIGLAIGKRATHTADDLLRESDT
                     AMYISKQRGRNTWHIFSGIMKENSQEVLSIISGLRNAVDKNEFCLFYQPIVEETGKIK
                     GLEALLRWKNEGKNIPPNVFIPIAEKHGMISSIGKWVFREACRMETYLRTELGKNSPY
                     ISVNLSAMQLNDKDTVSEFFRILKEEKTDPAQILLE"
BASE COUNT          663 a          375 c          455 g          488 t
ORIGIN      
        1 cacggaatat ggcggagaaa ttttaggaga agaatatttt ccaataggaa ttgataaaaa
       61 tgatatacaa aaccttatca cagacatttc caagtccggt accgacgtat tcgttcctta
      121 ttttgccgga tcggatcaga tcagcctttt gaatgaattt cattcccggg gactgaaaaa
      181 gaaaatggct gtagtaatgg ggcattatga tgaggcaatg gtaagccttc tcgctccgga
      241 agtaagggag gggttttact cttcaaatac atattttatg acagtgaata ctgcaaaaaa
      301 cagagaggtc ctcaaaaagc tttctgcatt tccaggagtg accggaatct ggccggatgg
      361 aaacggaata ctcacaaatt ttggtgaagg cacatatatc tgtatgcatg catttgcaat
      421 gggtgcagaa agagcaggca gtgtcgaaac ggaagcgttg accgctgcac ttgaaaaaac
      481 agtcattgag gcccctcagg gaacagtcag aatggatgct gcaacacatc atgcagaggt
      541 gcattcctat ctttctgtat gcacaaagga gggaatattt tccatcataa aggacttcgg
      601 cccctctgcc cctgtcattc cccaaagaca cagatctctt attccagaaa ataaaccgaa
      661 agaaacaaat ttttcttctg agattctgaa taaaaatcta agctttgtat cccaaagtat
      721 actcagagcg atagatgtat ccgtttttac agcagacgaa aacggggtga tcatccaggc
      781 aaacagggga gcctctgaaa cattcggcta ctcggaagag gagtttatcg gaatgtcagt
      841 caatatgctc ctcccgcctc atctgagatc agcacatatg cgccatatga aagaattcat
      901 ccatgacggg aaagccgaaa tcagaatggg aaaaagagga gagattgcag gatacagaaa
      961 agacggaact ttttttcccg cagaagccag tgtatccaag tttcaagctg agggtaaatg
     1021 gatactggtc acgactctga ttgatgtcac tgacaaaaaa aaatcggagg atgagctgaa
     1081 atggaaggcg actcatgatc ctttaaccgg actgccgaac agaaccctga taagggacag
     1141 gctctcagct gcacttcaaa gaacagaaaa gacaggaatg aacatcggtt taatgttcat
     1201 tgatctggac aattttaaac ttgtaaatga cagccttggt cacagcacag gagatgatct
     1261 gcttgtgcga attgcagata ttctgacgga aaatgtcaga ccgggggata cagttgcaag
     1321 acttggcgga gatgaatttg ttattttatg cgagcatatg gaaaacaagg aagaactgtc
     1381 tgggtttgct gaaatattga ataatgcatt aaggatatcc tttaattatg aaggaaagga
     1441 agtatcaact tcagcaagca tcggtcttgc aataggaaaa cgagcaacac atacagcaga
     1501 tgatcttctc agagaatcgg atacagccat gtacatctcc aagcagagag gaagaaatac
     1561 gtggcatatt ttctctggaa ttatgaaaga gaattctcag gaagttctca gtattatttc
     1621 aggcctccgt aatgctgttg ataaaaatga gttttgtctt ttttatcagc cgatagtgga
     1681 agaaaccgga aaaatcaagg gtctggaagc acttctcaga tggaagaacg aaggaaagaa
     1741 tattcctccg aatgtattca ttccaatcgc agaaaagcat gggatgatct cctcaattgg
     1801 aaaatgggtt ttcagagaag cctgcagaat ggaaacatat ctcagaacag agctgggaaa
     1861 aaacagccca tacatttcag tgaatctttc cgcaatgcag ctgaatgaca aagatactgt
     1921 cagtgaattt ttcagaatac tcaaagaaga aaaaactgat cctgcacaga tacttctgga
     1981 a
//
LOCUS       DAOQGJ010000715         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39439_length_1981_cov_2.736241, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000715 DAOQGJ010000000
VERSION     DAOQGJ010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39439_length_1981_cov_2.736241"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK683_11375"
     CDS             complement(<1..304)
                     /locus_tag="PK683_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNH09095.1"
                     /translation="MKIIFTAAIFFSLQFCAGTEIKTKTAEYKQDSKDYEGYFAEPAN
                     LKGKVPGVLLIHEWTGLGDHVKGRANQTAALGYVSFAMDMYGKGIRVEDHDHEKAGK"
     gene            574..1179
                     /locus_tag="PK683_11380"
     CDS             574..1179
                     /locus_tag="PK683_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09096.1"
                     /translation="MQKKIYQMFILLIVSFFILGQGISAQESGTRESYGSDWKYLGYS
                     ILAPGLGQFKKDRPVYGSIALGGFVLLTAHMALSADAYYAAEKNYQMKSVTAAAFTGA
                     LTQSGSISSSQAMLMNIRMSSMAFAPYNAAKENANNAVIVLGIFYIAQAFNAFFLNPG
                     GSTAENPLEKSFLYGLDLKVSPERTAFSTGTNYQMSYGWRF"
     gene            1179..>1981
                     /locus_tag="PK683_11385"
     CDS             1179..>1981
                     /locus_tag="PK683_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNH09097.1"
                     /translation="MKLSKLFIVLLLTVGCFKTKRSSFDPSTPVGTLNALFRVLNSSS
                     DYELEPVLTYSSSSYTYYTRIEFETPATVSNVKSCSVSPSLPAGIILDTTNCKISGTP
                     TSASSAVNYTVTAKNITKTKTAVLSISVEKIYTVFVTSSFTNGSFTDSVCSSDFNKPT
                     FGTYKAMIVRSTRRACSTANCSGGASENLDWVFRPNARYYKDTSSLFIKFVLETDSSG
                     IFTSISTPFSNSSNKVWTGLNSNFTTSANTCSNWTNNTGGFQGSYGFSDS"
BASE COUNT          535 a          416 c          414 g          616 t
ORIGIN      
        1 gtttccctgc tttttcatgg tcatggtctt cgactctgat tccttttccg tacatgtcca
       61 ttgcaaagga aacatatccg agtgctgcag tctgatttgc tcttcctttg acatggtctc
      121 caaggccagt ccattcatgt atgagaagga ctcccggaac ctttcctttc agatttgcag
      181 gttcagcaaa gtagccttca tagtccttgc tgtcctgttt atactctgcg gtttttgtct
      241 tgatttcagt tcctgcacag aactgaagag aaaagaatat tgctgctgta aagatgattt
      301 tcattcattg cttcctatag tgtgaatttt aggaacagga actcataaga gccttttcag
      361 tcaagaaaat acagcaggct ccgtttcatt caacgaatta tccggatcga gtcagtcaga
      421 aaaaacttga tttatgcata taattccgga tagaatcaga ggaattgtgt caaacggttc
      481 tgagaaaatt ttttgtttga catgatatat gcttatagaa tgtttttcga gacagttttt
      541 taactgtttc attcattttt tcaggagaca gaaatgcaga aaaaaatcta tcagatgttc
      601 atccttctga ttgtcagctt ttttatactc gggcagggaa tcagtgcaca ggagtccggc
      661 acaagggaga gctacggctc ggactggaaa tatctgggtt attcaatcct tgctccaggt
      721 ctcggacagt tcaaaaaaga cagaccagtc tatggatcga ttgcactggg aggttttgtt
      781 ctgctgactg cccacatggc attgtctgca gatgcatatt atgcagcgga aaaaaactat
      841 cagatgaaaa gtgtgactgc agctgcgttt acaggtgccc ttacccagag cggttccata
      901 tcatcctctc aggcaatgtt aatgaatatt cggatgagca gtatggcatt tgctccatac
      961 aatgctgcga aggaaaatgc aaacaatgca gtgattgtcc ttgggatatt ttatattgca
     1021 caggctttca atgctttttt tctgaatccg ggaggaagca cggctgaaaa tcctttggaa
     1081 aaatccttcc tgtacggtct ggatctgaag gtgtctccgg aaaggactgc attcagcacc
     1141 ggaacaaatt accagatgag ttatggatgg aggttctgat gaaactgtcg aaattattta
     1201 ttgtcctgct acttactgtc ggatgtttta agacgaaaag atcgtccttc gatccctcga
     1261 cccctgtcgg aactctgaat gctctttttc gagttttaaa ttcctcttca gattatgagc
     1321 tcgagccggt tctgacatac agcagttctt cctatacata ttatactagg attgaatttg
     1381 aaacgcctgc aactgtaagt aatgtaaagt cctgttccgt ttctccttct cttcctgcag
     1441 gaatcattct ggatacgacg aactgcaaaa tttcaggcac accgacttct gcttccagtg
     1501 cagtaaatta tactgtgact gcaaaaaaca ttacaaagac aaaaacagca gttctgtcca
     1561 tttcagtgga aaaaatatat actgtcttcg tgacgagttc ctttacaaac ggaagtttta
     1621 ctgattctgt ctgctcttct gatttcaaca aacctacttt tgggacgtac aaagcaatga
     1681 ttgtaaggag cacaagacgg gcctgttcca ctgcgaactg ttccggaggc gcctctgaga
     1741 acctcgactg ggtgtttcgg ccgaatgcaa ggtattacaa agatacttcg tccctcttta
     1801 ttaaatttgt tctggaaacg gacagttccg ggatatttac ttcaatttca actccttttt
     1861 cgaattcgtc caataaggtc tggactggtc tgaattctaa ttttacaact tctgcaaaca
     1921 catgttccaa ctggacaaat aatacaggag gatttcaggg ttcctacggg ttttccgact
     1981 c
//
LOCUS       DAOQGJ010000716         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39451_length_1980_cov_4.289870, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000716 DAOQGJ010000000
VERSION     DAOQGJ010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39451_length_1980_cov_4.289870"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK683_11390"
     CDS             complement(<1..413)
                     /locus_tag="PK683_11390"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH09098.1"
                     /translation="MYNPELKIERTSKGELLIIAPAGGETGNRNSMITYFVKSYEIRK
                     GGGVSFDSSTGFLLPNKAIRSPDAAWIRLEKWNSLTSEEKRRFVPICPDFLIELLSES
                     DELGQTQLKMNEWMEKGCSLAWLIDPFQKKTIVYR"
     gene            complement(502..831)
                     /locus_tag="PK683_11395"
     CDS             complement(502..831)
                     /locus_tag="PK683_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020763567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNH09099.1"
                     /translation="MFPKHIQKKLKEIRKEIRKTVPDAEEKISYQMPCFALNGILVWY
                     AGYEKHIGFYPSSSGIAKFQKEISKYKNSKGAVQFPLDEPLPMELIKKIVLFRVKENS
                     AKKEKKT"
     gene            complement(1042..1287)
                     /locus_tag="PK683_11400"
                     /pseudo
     CDS             complement(1042..1287)
                     /locus_tag="PK683_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
     gene            complement(1493..1723)
                     /locus_tag="PK683_11405"
     CDS             complement(1493..1723)
                     /locus_tag="PK683_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09100.1"
                     /translation="MERKYFPVDLFLVGSGEEFKKYAVKKYQITVQGKIIEVKDGWLK
                     YEYEAVGKIFSGSVSKNFFRFLNRQGEHQVFQ"
     gene            1729..>1980
                     /locus_tag="PK683_11410"
     CDS             1729..>1980
                     /locus_tag="PK683_11410"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09101.1"
                     /translation="MLEDLDYGSIHFLQDTKLLTDQLAEFRLPIMKRLPLGIQTFSKL
                     ISDSHYYVDKTEFVYKLSKIGTYFFLSRPRRFGKSSFLDT"
BASE COUNT          537 a          425 c          352 g          666 t
ORIGIN      
        1 ttcctgtaaa caatggtttt tttctgaaat ggatcaatca gccaggcgag agaacagcct
       61 ttttccatcc attcattcat tttcagctga gtctgaccaa gctcatccga ttcggaaaga
      121 agttcgatga gaaaatcagg acaaatcgga acaaatcttc ttttttcttc cgatgtcagt
      181 gaattccatt tttccagtct gatccaggct gcatccggag aacggattgc cttattcggc
      241 agaagaaaac cggttgagga atcaaaagac accccgcctc cttttcttat ttcataagat
      301 tttacaaagt aagtaatcat tgaatttcga tttccagttt ctccgccggc cggggcaata
      361 atcagaagtt ctcctttgga agttctttca attttcaatt caggattgta cacacagaaa
      421 ttatagagac gattatcatc cctatggaat tctgagaggc agagctggca tatttttgtt
      481 cgtatgcaga aaacgaatct gtcaagtctt tttttccttt ttggctgaat tctccttgac
      541 tcggaaaagc acaatttttt tgatcagttc catcggcaga ggttcatcca gaggaaactg
      601 aactgctccc ttagaattct tgtatttgga aatctccttc tggaattttg caattccgct
      661 ggaagaagga taaaaaccga tatgcttctc atatcctgcg taccagacta aaataccgtt
      721 taatgcaaaa cagggcatct gataactgat tttttcttca gcatcaggaa cagttttccg
      781 tatctctttt ctgatctctt ttaatttttt ttggatatgt ttcggaaaca tctcaatgta
      841 ttcatccggc gtggagaatt ttttcttttc tgaatccttg gtcttttccg caggagaagt
      901 tttcagttcc agtttaatgt aaggatgagt ggagatgata tcgaggattg tatcaaagag
      961 agtctccccc tgaccctttt caacgagctt cagtcttttt ctcagatccg gcagattgga
     1021 aggatgacat tttttcatta tatcattcaa atatttaatg gtttccttat gtcccaattt
     1081 ttctctgctg aaatcagaat cattccagac aatcagaagt tctttcccgt atccgtgtat
     1141 gccaaaatct cctctcagta tatcattgaa tgcgtccaga tttcttccaa tcttccagcc
     1201 aagaccggat gtcagcttct tttccacttc atcgtaaaac gatttcagat tcttaatgtt
     1261 cttgccttcc aagactgctg ttttcatagt tattttctcc ttttttattc accttacgcc
     1321 tatggctgca ggtgggcgtt tggggcagga acgcctgccg gcggcaagga cagcggctgc
     1381 caaaggaata taaggcttat actgatctgt ctgcatcgag tcctgtatat ctgaatgatt
     1441 catctattga actgctctca gccttaaaaa taaagctgaa tatcccggat atttactgga
     1501 atacctgatg ctctccctgt ctattaagaa atcggaaaaa attttttgag acactgcctg
     1561 aaaatatttt tccaactgct tcatattcat atttcagcca tccgtctttg acttcaataa
     1621 tttttccttg gacagtaatt tgatattttt ttactgcata ttttttaaac tcttcaccgg
     1681 atccaacaag gaatagatca accggaaaat attttctttc caagtctgat gcttgaggat
     1741 cttgattacg gaagcatcca ttttttacaa gatacaaaac tcttgactga ccaattggca
     1801 gaattcagac tgcctattat gaagagactg cctcttggaa ttcagacctt ttccaaactc
     1861 atctcagaca gccactacta tgtagacaaa actgaatttg tatataaatt atcaaagatc
     1921 ggtacatact tttttctgag ccgtccccgc cgattcggga aaagttcttt tctggacaca
//
LOCUS       DAOQGJ010000717         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39534_length_1977_cov_4.172737, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000717 DAOQGJ010000000
VERSION     DAOQGJ010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39534_length_1977_cov_4.172737"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1931)
                     /locus_tag="PK683_11415"
     CDS             complement(<1..1931)
                     /locus_tag="PK683_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH09102.1"
                     /translation="MDLQKEWKAKEGIHKDSVPDETWQSFEKTTIRFSKIKPKEGNVQ
                     SYTFYKEVIFPEMDYSSLQDSVSILIPNIMNMYEFYFDGNLLSSRGKIENGKIVRYGY
                     RRNIVVVIPQKLLSAGRHKIHIIASSAANEDIELTGNASMTADLHSRHIEAVSERIPL
                     MIIFMYIFVGFYHLLLFLKRTQEKYNLFFGLFCLDIFCYLFLRTEVSYSLDLDPFLVT
                     RIEYMFLFLLSPLALVFFDNFFENKVTRFSKAFLGLCGFMGLTVLFMPRYIASKILLG
                     WQFLALASFIYFIITIIRAIRNRHQDSRRLLLAFSIFIFAAIYDVVGATNLIPGIVNK
                     ELMKYGFFTFIIGIAFVLANRFLRLHKQVEDLNSNLEQKVEQRTKELQNSLDEIRFLK
                     FQQDGDYFLTTLLINPLMTNNTRSDNITVEYYVKQKKNFMFKNRMYEIGGDICISDMI
                     TLRDKKYVAFINGDAMGKSIQGAGGALVLGVIFKSILERTKSYGKQLNVYPERWLKNC
                     FSELHNVFVSFDGSMLVSVVMGLVDESTGMLYFINAEHPFTILYRDGKAEFLETKLMH
                     HKIGMQGLEGSVRIQTFQMEPQDIIIIGSDGRDDIVISKDGQGNRQINEDENLFLRKV
                     EQGEADLKHITELLLKEGEL"
BASE COUNT          596 a          428 c          377 g          576 t
ORIGIN      
        1 agttctcctt ccttcagaag aagttcagtg atatgcttga ggtcagcttc tccctgttcc
       61 accttccgga gaaaaagatt ttcatcctca ttgatctgcc tgttcccctg cccgtccttg
      121 gagatgacaa tatcatccct tccgtcagat ccgatgatga tgatgtcctg aggttccatc
      181 tgaaatgtct gtatcctcac gcttccttcc agtccctgca tcccgatctt atggtgcatc
      241 agttttgttt caagaaactc agcctttccg tcccggtaca gaattgtaaa agggtgttcc
      301 gcattgatga agtacagcat tcctgtggat tcatccacaa gacccataac aacggagaca
      361 agcatacttc cgtcaaagga gacgaagaca ttgtgaagtt ccgaaaagca gtttttcagc
      421 catcgttcag gatacacatt cagctgcttt ccgtaacttt tggttctttc aaggatggat
      481 ttgaaaatca ccccaagtac aagagcacct cctgctccct gaatggactt gcccatcgca
      541 tccccgttga tgaacgcgac atacttttta tcccggagag tgatcatatc cgatatgcag
      601 atgtctcctc caatttcata catcctgttc ttgaacatga aatttttctt ctgtttcaca
      661 tagtattcaa cggttatatt gtcacttcga gtattatttg tcatcagagg atttatgaga
      721 agagtggtca gaaaatagtc tccgtcctgc tggaatttca ggaatctgat ttcatccagc
      781 gagttctgca gttcctttgt tctctgttca accttctgct cgaggtttga attcagatct
      841 tcgacctgct tatgaagtct taaaaacctg tttgccagaa caaatgcaat tcctatgatg
      901 aaagtaaaga atccgtattt catcaattcc ttattcacta ttccagggat taaatttgtt
      961 gcaccgacta catcataaat tgccgcaaaa atgaaaatgc tgaatgccag aagaagtctt
     1021 ctggaatcct gatgcctgtt tctgatggcg cggataattg taatgataaa atatataaaa
     1081 ctagcaaggg caagaaactg ccatcctaga agaattttag aagcaatgta tctcggcata
     1141 aaaagaacag tcagccccat aaaaccgcag agtccaagaa atgctttgct gaaccgggtc
     1201 actttatttt caaaaaaatt gtcaaagaac acaagggcaa gaggactcaa aaggaagagg
     1261 aacatatact caattctcgt gacaagaaaa ggatccaaat ccagtgaata actgacctca
     1321 gtcctcaaaa acagatagca gaaaatgtcc agacagaaga gtccgaagaa gagattgtat
     1381 ttttcctggg tccgtttcag aaaaaggaga agatgataga aaccgacgaa aatatacata
     1441 aatataatca tcagaggaat tctttcagaa acagcctcga tatgcctgga atgcaagtct
     1501 gcagtcatac tggcatttcc tgtcagctca atgtcttcat ttgctgcgga actcgctatg
     1561 atatgaatct tatgtctgcc tgcactgaga agcttttgag gaattacaac aactatgttt
     1621 cttctgtacc cgtacctgac aatttttccg ttttcaatct tacctcttga agaaagaaga
     1681 tttccgtcga aatagaactc atacatattc attatatttg gtataagaat ggatacactg
     1741 tcctgaagag aggaataatc catttccggg aaaatgacct ctttataaaa cgtataggac
     1801 tgaacattgc cttctttggg ttttattttt gaaaaacgaa ttgtcgtttt ttcaaaagac
     1861 tgccatgttt cgtcagggac agaatcctta tgtatgcctt ctttcgcttt ccattccttc
     1921 tgcagatcca caggaaaaga aaagacagag aaggaaaaga ataaggacag gactgtc
//
LOCUS       DAOQGJ010000718         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39545_length_1977_cov_3.043704, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000718 DAOQGJ010000000
VERSION     DAOQGJ010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39545_length_1977_cov_3.043704"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PK683_11420"
     CDS             <1..832
                     /locus_tag="PK683_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH09103.1"
                     /translation="AGFERKLQKGENSPLQAVLDGSDGSSASTSLGYMLRMFIEHQWK
                     TWQKTPDIRFYSVTPPPLPKLELQTRVYFNPEMESRFFLLPGIMTMILTIIVTLLTAM
                     GITREKESGTFDQLIVSPIRGWELILGKTLPYLLIGLADLFLVTVVSRYVFQLQMSGS
                     FFSFIVLNTIYIICMLGLGLFISTVSRSQGQAMMTVFGFLFPFIILSDFFFPVDNMPL
                     IIRRLTMLNPMKYAMVAQREIFLKGNGLAFLWDSILILALFSLIFLGYGAYRFRAQTD
                     "
     gene            1013..>1977
                     /locus_tag="PK683_11425"
     CDS             1013..>1977
                     /locus_tag="PK683_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNH09104.1"
                     /translation="MAMSFINEDQQIKNFIQKISDKAAAGGYNISDIVSEENGRTLQY
                     VDIVMEGGGVLGVALIGYIYALETAGIRFLSAGGTSAGSIAALLLTACADRQEAKTEK
                     LSEILFKLDLSEFIDGDADAKDFSQFIGSGELDDHRIKLMWKGAQVLDNLFNDIGLNP
                     GRSFFEWVDGHMKNFGTDTLEKLEDRIQRIPNLHNKITGSSLDKINAELCIVAADIIS
                     ETKVIFPKMAPMYWDDCRKTSPAHFVRASMSIPFFFHPMKIDRLSEITDVRSRWKDLA
                     NFDNPPESAVFADGGVFSNFPIDLFHQEGVPDAPTFGVKLGAKKR"
BASE COUNT          570 a          405 c          451 g          551 t
ORIGIN      
        1 agcaggtttt gaacgaaagc ttcagaaggg cgagaattct cctcttcagg cagttcttga
       61 cggaagcgac ggaagttcgg caagtacatc ccttggatat atgctcagaa tgttcattga
      121 gcatcagtgg aaaacctggc agaaaactcc tgacattaga ttctattctg tcactccccc
      181 tccccttccc aaactggaac ttcagacaag ggtctatttc aatccggaaa tggagtccag
      241 attttttctt cttccgggaa ttatgacaat gatactgaca attatagtaa ctttgcttac
      301 tgcaatggga atcacaagag aaaaggaaag cggcacattc gatcagctga ttgtctctcc
      361 gatcagaggc tgggaactta ttttagggaa gacactcccc tatctgctga taggactggc
      421 agatcttttt cttgtcacag tggtttccag atatgttttt cagcttcaga tgagcggatc
      481 attcttcagc ttcattgtat tgaatacaat atatataata tgtatgctcg gattgggact
      541 ttttatttcg acagtaagca ggtctcaggg acaggctatg atgacagtat tcggatttct
      601 ttttccgttc atcattcttt cggatttttt ctttccagtg gacaatatgc ctcttataat
      661 acgtagactt acaatgctga atccgatgaa atatgctatg gtcgcccaga gagaaatttt
      721 tcttaaagga aacggactgg cttttctctg ggattccatt cttattctag ccctgttctc
      781 tttaatcttc ctcggatacg gagcctacag attcagagct cagacggact gagatgagag
      841 ttcataaaaa cattggactg aatctcttcg gacagaatca tgcatcagaa atctgcagtt
      901 tcagtcttct ccagaaatga actggaccaa agatatgtcc aaaacttgac ttgcattaaa
      961 atggatttct gattctttag taggatgatt ggattatttt cgggggaaag ttatggcaat
     1021 gtctttcata aatgaagatc agcagataaa gaattttatt caaaaaattt ctgacaaagc
     1081 tgctgcagga ggatataata tttccgacat agtgtccgag gaaaacggca gaactctgca
     1141 gtatgtggac atagtgatgg aaggaggcgg agttctcgga gttgcactga tcggatatat
     1201 ctatgcattg gaaactgcag gcatacgatt cctgagtgcc ggcggaactt cggcaggatc
     1261 gattgcagcg cttcttctga ctgcctgtgc cgacagacag gaagccaaga cggagaaact
     1321 ttcggaaatt ctcttcaaac tggatctttc tgaattcatc gacggcgacg cagatgcaaa
     1381 ggacttcagt cagttcatcg gaagcgggga gctggatgac cacagaatca agctgatgtg
     1441 gaaaggagcc caggttctgg acaatctttt caatgatata ggcctgaacc caggaaggag
     1501 cttctttgaa tgggtcgacg ggcatatgaa aaatttcgga acagatacac tggaaaaact
     1561 tgaagataga atacagcgta tcccaaatct tcacaataaa atcacaggaa gcagtctgga
     1621 taaaatcaat gcagagctct gcattgttgc agcggatatt atcagcgaga caaaagtgat
     1681 atttcccaag atggcgccta tgtactggga tgactgcaga aagacttctc ccgcacattt
     1741 tgtaagagcc tctatgtcca tcccgttctt ttttcatccg atgaagattg acagactgag
     1801 cgaaatcaca gatgtacgca gcaggtggaa ggaccttgca aattttgaca atcctcccga
     1861 atctgctgta tttgccgacg gcggagtatt ttcaaacttt ccgattgatc tatttcatca
     1921 ggaaggagtt cccgacgccc ctacgttcgg agtaaaactc ggggccaaaa aaagaag
//
LOCUS       DAOQGJ010000719         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39547_length_1977_cov_3.007804, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000719 DAOQGJ010000000
VERSION     DAOQGJ010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39547_length_1977_cov_3.007804"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK683_11430"
     CDS             <1..285
                     /locus_tag="PK683_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNH09105.1"
                     /translation="KKIISRVREKNPEVKILLVPMKVFPNYGPKYGKQFEKLYKEISE
                     EENVPLSPFLLEKVAGIRKLNLPDGIHPTAEGHKLLFSTMYEPVKNLLKD"
     gene            complement(263..1429)
                     /locus_tag="PK683_11435"
     CDS             complement(263..1429)
                     /locus_tag="PK683_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNH09106.1"
                     /translation="MYSDRFQFSPEELQIHSKWKKLVNSNAERFDLTVSNPTRTGLSF
                     PETEILSCFQNSKILDYQPDPRGSIQCREIISEYYQEKGRSVDPEDIFLTSGSSESFS
                     FLLRLFCNPGDEIICFSPSYPLMEYLAFMENVMIRYCDKDFVGTDQEGFAEWKINFDR
                     LEGNITERTKMIIVVQPNNPSGEILEKEELRILEETAERRGIILVFDEVFSDYIFKGN
                     KICLETTSADSVFLGGISKSLGLPQMKLSWMYLKGSREFRKKTGDYLEIITDSFLSVG
                     TPVQAAFPELWKLRKKLNSVIQKRLKESLGILKNIKYPKPLFPSGGWYIPLLYSSSIL
                     TDDELAEKLVEERFTLTHPGTMFGGFRNRTVLAVCLLSYDFQKEGCLLSLSADF"
     gene            complement(1435..>1977)
                     /locus_tag="PK683_11440"
     CDS             complement(1435..>1977)
                     /locus_tag="PK683_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH09107.1"
                     /translation="DLFYRLNVVNITMPPLRERPEDISILVSHFIDKYNKDNNKSLKG
                     ITKEAHKLLLKYQWPGNVRELENMIERSVVLTQKDVLDETDFTEILEKLNTEERAEIL
                     KPQQNVSVQSTEEMAAIQTSNIDSLDGKAMETVISEVETRLIQYAMKKFRYTKTRVAK
                     FLGINRNTLDKKIKELNIEY"
BASE COUNT          537 a          404 c          399 g          637 t
ORIGIN      
        1 aaaaaaataa tcagcagggt tcgggagaaa aatcccgagg tgaaaatcct gcttgtgccg
       61 atgaaagtgt tccctaatta cggacccaag tacggaaagc agtttgagaa gctgtataag
      121 gaaatcagtg aggaggaaaa tgttcctctg tctccgtttc ttctggaaaa agttgcaggc
      181 ataagaaaac tcaatcttcc cgatggaatt catccgactg cagaaggaca taagcttctg
      241 ttttctacaa tgtacgaacc tgtcaaaaat ctgctgaaag actgaggagg catccttctt
      301 tctgaaaatc gtaagagagt aggcagacag caagtaccgt gcggtttctg aatcctccga
      361 acatagtgcc gggatgagtg agtgtaaacc tttcttctac aagcttttct gcaagttcgt
      421 catcagtcaa tattgaagaa gaatataata aaggaatata ccatccgccg gaagggaaaa
      481 gaggtttggg atattttata tttttcagta tgccaagaga ttctttcagc cttttctgaa
      541 tgactgaatt cagctttttt cgaagtttcc agagctcagg gaatgctgcc tgaacaggag
      601 ttcctaccga aagaaaggag tccgtgatta tttccagata atctcctgtt tttttcctga
      661 attctctgct tcctttcaaa tacatccagc tgagtttcat ctgtggaagc cccaggcttt
      721 tggatattcc tccgagaaag actgaatcag ctgaagttgt ttcaaggcag attttgtttc
      781 ctttgaatat atagtctgaa aatacttcat caaaaacaag aatgattccg cgcctttctg
      841 ccgtttcttc caggattctc agttcttcct tttccagaat ctctcctgaa ggattgttcg
      901 gctgcacaac gatgatcatt ttcgttcttt ccgtaatgtt tccttccagc cggtcaaaat
      961 tgattttcca ctcggcaaat ccttcctgat ctgttccgac aaagtccttg tcgcaatatc
     1021 ttatcattac attttccata aatgcaagat attccatcag aggataagat ggactgaagc
     1081 agatgatttc gtcgcccgga ttgcagaaca gccgaagaag aaaggaaaag ctttcagaac
     1141 tgccggaagt caggaatata tcttcagggt cgacagatct tcctttttcc tggtaatatt
     1201 cagaaatgat ttctctgcat tgaatgctac ctcttggatc aggctgataa tccagaattt
     1261 tactgttttg gaagcaggaa agaatttctg tttccgggaa agaaagacct gtgcgtgtcg
     1321 gatttgagac agtcagatcg aatctttctg cattagaatt gaccagtttc ttccatttgg
     1381 aatgaatctg aagttcttca gggctgaatt gaaatctgtc agaatacaca gctctcaata
     1441 ttctatattc agttccttga ttttcttatc gagagtattt ctgttgattc ccaggaattt
     1501 ggcaactctt gtcttggtat atctgaattt cttcatagca tactgaatca gtcttgtttc
     1561 cacttcgctg atgacagtct ccattgcctt tccgtcaagc gagtctatat ttgaagtctg
     1621 aatggctgcc atttcttctg tggactgaac ggacacattc tgctgaggtt tcagtatttc
     1681 tgcacgttct tctgtattca gtttttccag aatctcagtg aagtcagtct catccagaac
     1741 atctttctga gtaagaacaa cagatctttc gatcatattt tccagttctc tcacatttcc
     1801 gggccactga tatttcagaa gaagcttatg agcttctttt gtgattcctt tcagagactt
     1861 attgttgtct ttgttatatt tatctataaa atgggataca agaatggaaa tgtcctccgg
     1921 tctttccctt agaggaggca ttgttatgtt taccacattc agtctgtaga acaggtc
//
LOCUS       DAOQGJ010000720         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39572_length_1976_cov_3.950547, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000720 DAOQGJ010000000
VERSION     DAOQGJ010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39572_length_1976_cov_3.950547"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PK683_11445"
     CDS             <1..901
                     /locus_tag="PK683_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09108.1"
                     /translation="LNLILVPLTSLILPVLYAALILQYFSAPVITEILWVNLEILLRT
                     LALLAVEMGESLGFYREFGEGRQTVLNYYYIVILFIIFMFIINAYLSKDKASELKQRI
                     IRISVSFLCFTALILFCYFGYNTEDPRKYPSSELIFSSQNSFLYMKGREAFLSGSCKY
                     NSYPIGISIREKEVCKRAEVFHIDDETCISSVLKCRDRSKLFYISEKGKSRFSESTGF
                     ISEKGSISRLITEDSGKKIIFYAPHRDSIESLVYSTRSGNGKIAMQFSYRSRDSVDDW
                     NSNRGLLGIPYSWKFEDANKIIK"
     gene            complement(891..>1976)
                     /locus_tag="PK683_11450"
     CDS             complement(891..>1976)
                     /locus_tag="PK683_11450"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH09109.1"
                     /translation="PMKIYAVYTLRPAQALTIAGIIYSVAVLVRAGIKRKEGAWLFLS
                     GIIIFFLFIINDIMYSFGVLRTGTFANYGLLVFILVQAVLLSQRFSRSFLMSEKLSEQ
                     LTDLNQYLEEKVELRTRHLEEARQAAERERLKSEKLAESRKSLSIVGQMAAGVVHDIK
                     NSIMTIKSYSEAANSSEISEDERKKDLEIIIREIDKLGDMAYEILDFARGDIRLFKTE
                     VRVSEFLNEIKDVLYPDFEYCGIRLDVMTEADSVILIDRGRMRRVLINLANNAREAMS
                     ENRNDYRLQIRSEISDGKVRIVLSDNGTGISPQIQDRLFDIFITEGKTKGTGLGLFMC
                     RWVVELHEGTISFSTEFGKGTEFIITL"
BASE COUNT          555 a          434 c          365 g          622 t
ORIGIN      
        1 tctgaatctt atactcgttc cgctgacatc cctgatcctg cctgtcctgt atgctgctct
       61 gattcttcag tatttttccg cacctgtaat tactgaaata ttatgggtca atctcgaaat
      121 actcctcaga actctggctc ttctggctgt tgaaatggga gagtctctcg ggttttacag
      181 agaattcggg gaaggtagac agacagtact aaattattat tatattgtaa tcttatttat
      241 aatctttatg ttcatcataa atgcttatct ttcaaaggac aaagcttctg aattgaagca
      301 gagaattatc agaatttcgg tatcctttct gtgctttact gccctgattc ttttctgcta
      361 tttcggatat aataccgagg atcccagaaa atatccttcc tctgagctca tattctcttc
      421 acagaacagt tttctttata tgaaaggcag agaggctttt ctatccggtt cctgcaaata
      481 caattcctat ccgatcggca tttccatccg cgaaaaagaa gtctgcaaaa gagcggaggt
      541 ctttcatata gacgatgaaa cctgtatttc gtctgtactt aaatgcagag acaggtctaa
      601 gctgttttat atttcagaga aaggaaaaag cagattttct gaaagcactg gattcatttc
      661 agagaaaggt tcaatctcca gactgattac tgaggacagc ggaaagaaaa tcatttttta
      721 tgcaccgcac agagattcca tagagagtct tgtttacagc acaagatccg gaaatggaaa
      781 aattgcaatg cagttcagtt accgttcgag agacagcgtg gatgactgga attccaacag
      841 aggtcttctg ggaatacctt attcctggaa atttgaagat gctaataaaa ttataaagta
      901 atgataaatt cagttccttt tccaaattca gtgctgaagc tgatcgttcc ttcatgcagt
      961 tctactaccc atctgcacat aaacagacca aggcctgttc cttttgtctt tccttctgta
     1021 atgaatatat cgaaaagcct gtcctgaatc tggggtgaaa tgccagtacc gttgtctgaa
     1081 agaacgatcc tgactttgcc atctgaaatt tcagaacgaa tctgaagccg ataatcgttt
     1141 ctgttttccg acatcgcctc cctggcatta tttgcaagat tgatcagaac ccttctcatt
     1201 ctgcctctgt caatcaaaat gacagaatct gcttcagtca tcacatccag tctgattccg
     1261 cagtattcga aatcaggata tagtacatcc ttaatttcat tcagaaattc actgactctg
     1321 acttctgtct tgaaaagacg tatgtctcct cttgcaaaat cgagaatctc ataagccata
     1381 tctcccagct tgtcaatctc acgaatgatg atttccagat ccttctttct ttcatcttca
     1441 gatatttcgg aagagttcgc tgcttcggaa tacgatttga ttgtcattat cgaattctta
     1501 atgtcatgca caactcctgc cgccatctgt cctacaatgg aaagactttt tctggattct
     1561 gcaagctttt cagatttcag tctttctctc tcagcagcct gtcttgcttc ttccagatgc
     1621 cttgttctca gctcgacttt ttcttcaaga tactgattca gatccgtcag ctgttcgctc
     1681 agcttttcag acatgagaaa tgatctcgaa aatctctggg aaagaaggac tgcctggaca
     1741 agtatgaata caagaagtcc gtaattggcg aatgttcctg ttctcagaac tccaaaactg
     1801 tacataatgt cattaatgat aaacagaaag aaaataatga ttccggagag aaagagccat
     1861 gctccctcct ttcttttgat tccggctctg accagaaccg caactgaata gataattccg
     1921 gcaatcgtga gtgcctgtgc cggtctcagc gtataaacgg cataaatctt catagg
//
LOCUS       DAOQGJ010000721         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39583_length_1976_cov_3.116085, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000721 DAOQGJ010000000
VERSION     DAOQGJ010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39583_length_1976_cov_3.116085"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PK683_11455"
     CDS             <1..541
                     /locus_tag="PK683_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09110.1"
                     /translation="KIFDSAYEKNTDQIRFINIRPGKFIIESVCDTSAYNRSHIYFYY
                     DESIQKPKKRDQVKKYNSPPIIFFPTDPVFKNKENLHWGLASFIFKVGTRNFDSKTLT
                     LDAFVKGLGDGSTGHYTRYKFYPKTFRPELVFTIIRNREDHKEVYNYEKGILPSGKGW
                     RYISRKVKLKGFFNSDRNL"
     gene            642..1013
                     /locus_tag="PK683_11460"
     CDS             642..1013
                     /locus_tag="PK683_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09111.1"
                     /translation="MGAFQRSRQNELWASTMTIRDTFFVFLRFDFSGIATMVMSLGTV
                     CGFAVAAILAMVVGYFEKKPFCESKGLRYAKSCGIAILIGLAPMLAFSNDRLLKISDE
                     LALAFPWAALAVGLISLRISK"
     gene            1046..>1976
                     /locus_tag="PK683_11465"
     CDS             1046..>1976
                     /locus_tag="PK683_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09112.1"
                     /translation="MNKKNDNKERKYLSLLAILFTLSSPVFAKDDSLIIGVPDFVIRL
                     NEDSKDHTFQPEMPDILNEKRADGTLLIFIDPGMKTEYSNQSSPLSTLISYYNAVNRR
                     DYSSANNCLSSPYESEEQYQKWTDGYRNTKKVELFINYNESKIEGAAGSIFITIPTVL
                     ISYDGKRNRVEYEGSYTLNTSNQSKRKIWKIHSGKFRRFASLLIEESIKENAEREITY
                     FEKGYAFMRKILFPFHSEKKDPEFHEIYKKLQKEIRNKNKDYLFSLYDENIKCNFDSG
                     ENKETCIKDFNQEESFHNKKSKNDSNWIILNELL"
BASE COUNT          711 a          329 c          379 g          557 t
ORIGIN      
        1 taaaatattt gattctgctt atgaaaagaa tacagaccaa atcagattca taaacattcg
       61 acctggaaag tttatcattg aatcagtatg tgatacatcc gcttacaaca gaagtcatat
      121 ttatttttat tatgatgaat caattcaaaa accgaaaaaa cgcgaccaag ttaaaaaata
      181 caactctccc cctataattt tttttccaac tgatcctgtt ttcaaaaata aagaaaacct
      241 gcattgggga cttgcttcct ttattttcaa agtcggcaca cgaaattttg attcaaagac
      301 actgactctg gatgcatttg tcaaagggct tggagacgga agcacaggac attatactcg
      361 gtataaattt tatccaaaaa cttttcgccc ggaattagtc tttacaatta ttagaaacag
      421 ggaagatcat aaagaagtat acaattatga aaaaggcatt ctgccgtcag gcaagggctg
      481 gagatatatt tccagaaagg tgaaactgaa aggatttttt aattcagaca ggaatcttta
      541 aaaagaggaa aaaacatgaa aataataagc acagtggcat taactttaat tcttggatgt
      601 gcaggcgtcg ggggaatgcg aggatatgca gttataaatt tatgggagca ttccaaagga
      661 gccgccagaa tgagctgtgg gcaagcacaa tgaccataag ggatactttc tttgtcttcc
      721 ttcgtttcga cttctcaggg atcgctacca tggtaatgtc gttgggcact gtctgcggtt
      781 ttgctgtcgc agcaatactg gctatggttg ttgggtattt tgaaaaaaag cctttctgcg
      841 aaagcaaagg ccttcgatat gccaaaagct gcggcattgc cattttaatc ggtttagctc
      901 caatgcttgc gttttctaac gatcgacttc tcaaaataag cgatgaactt gcgctagcct
      961 tcccctgggc agcccttgca gtagggctga tttcactgcg catttcgaag taaaatttta
     1021 cctgctggat aataggagaa atgaaatgaa taaaaaaaat gataataaag aaagaaaata
     1081 tttatcgctt ttagcaattc tatttacgct ttccagtcct gtatttgcaa aagacgactc
     1141 ccttattatc ggtgtgcctg attttgtaat cagattgaat gaagacagca aggatcatac
     1201 gtttcagcct gagatgccgg acatactgaa tgaaaaaaga gcagacggaa cccttcttat
     1261 tttcattgat ccgggaatga aaacagagta ttcaaatcag agttctcctt tgagcacatt
     1321 gatttcctat tataatgctg ttaacaggag ggattattcc tctgcaaata actgtttaag
     1381 cagcccgtac gaatcggaag aacaatatca aaaatggaca gatgggtacc ggaatacaaa
     1441 aaaagtcgaa ctgttcataa attataatga atcaaaaata gaaggcgctg cgggttctat
     1501 ttttattacc ataccgactg tattaatttc atacgatgga aaaagaaaca gagtggaata
     1561 tgagggaagt tatacattaa atacatcgaa tcagagtaaa agaaaaattt ggaaaattca
     1621 ttccggaaaa tttcgaaggt ttgcatcctt attaatagaa gaatcaatca aggaaaacgc
     1681 agaacgggag ataacttatt ttgaaaaagg atatgcgttt atgagaaaaa ttctattccc
     1741 tttccatagc gaaaaaaaag atccggaatt ccatgaaatt tataaaaaac ttcagaaaga
     1801 aatcaggaat aaaaataaag attatttatt cagcctgtat gacgaaaaca taaaatgcaa
     1861 ttttgactca ggcgagaaca aagaaacatg cataaaagat ttcaatcagg aagaaagctt
     1921 tcataataaa aaatcaaaaa atgattcaaa ttggattatt ctgaatgaat tattaa
//
LOCUS       DAOQGJ010000722         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39596_length_1976_cov_2.144196, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000722 DAOQGJ010000000
VERSION     DAOQGJ010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39596_length_1976_cov_2.144196"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK683_11470"
     CDS             complement(<1..366)
                     /locus_tag="PK683_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09113.1"
                     /translation="MNLNEDEIKNIYGREELAAGAERIFGASAEFFLSLKTEDFFKNF
                     NGSWSAEKNLRHLIKSTLPIYLGIKSPKFLLQLLFGKGRAKSRTYSEIREDYLSRLAS
                     GSGAGIFEPLFNSAGKPEEQ"
     gene            complement(356..1033)
                     /gene="queC"
                     /locus_tag="PK683_11475"
     CDS             complement(356..1033)
                     /gene="queC"
                     /locus_tag="PK683_11475"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNH09114.1"
                     /translation="MTSKADKSNAVVLFSGGLDSTVCLYYALKKRKRPIALSFDYSQR
                     HRVELKRAAAITYRLGVEHRVIRLDTEVFLGSSLTEKQLKVPKGSVSDGKIPNTYVPG
                     RNMLFLSFAVSVAEGRGCREIYIGVNALDYSGYPDCRPEFISAFQNAADIGTKAGNSG
                     RKIKIITPLQEMTKKDIILLGKKLNVPFDMTHSCYDPDRNGRPCGKCESCLLREKGFK
                     EALGDES"
     gene            complement(1017..>1976)
                     /locus_tag="PK683_11480"
     CDS             complement(1017..>1976)
                     /locus_tag="PK683_11480"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH09115.1"
                     /translation="QPMGKVSGDYYDLFRFPNYLFVLLADVSGHGVPAALITMSAKQS
                     FSAFIEEHLEPDEIFRKVNLDLVEKVKTSDYLTAFMLKIDSKNRITYSNAAHPRAVYY
                     VSSRNEYELLDTGGIFIGSLEEAGDLYENSTMRLHSGDRIYLYTDGLVEHKNRQGQEF
                     GLDRFIECLKRTSGDSLGEQITECVRSLEKFMDGAPVRDDVSMLVLELETSWTRFIEV
                     YNAGIKLLRSRNLTEAQDMLWKAYNIIPSYINLYFHLAFVFYHLNDLVRAEEMIRNFL
                     KEKPKDRAGLQLAINILSKLGRDSEAENYNNVLKEISPDDVKG"
BASE COUNT          512 a          481 c          402 g          581 t
ORIGIN      
        1 ctgctcttcc ggtttaccgg cagaattgaa cagaggttca aatattcctg caccgcttcc
       61 tgaggcaagt ctggaaagat agtcttctct gatttcactg tacgttctgg attttgccct
      121 gccttttcca aacaggagct gcagaagaaa cttgggagac ttgattccca gataaatggg
      181 aagagtggat ttgatcagat gtctcagatt tttttcagcc gaccatgaac cgttaaaatt
      241 cttgaaaaaa tcttctgttt ttaatgataa aaagaactca gcggaggcac cgaaaatcct
      301 ttctgcacct gccgcaagtt cttctctgcc gtatatattt ttaatttcat cttcattaag
      361 attcatcacc gagggcctcc ttaaaaccct tttcgcgaag caggcagctc tcgcattttc
      421 cgcagggcct gccatttctg tccggatcat agcaggaatg agtcatatca aaggggacat
      481 tcagtttttt tccaagcagt atgatatcct tcttggtcat ttcctgaagg ggtgtgatga
      541 tctttatttt tctgccggaa tttcctgcct tggttcctat gtctgccgca ttctgaaatg
      601 cagagataaa ctcgggcctg cagtcaggat aaccggaata gtccagagca ttcactccta
      661 tgtaaatttc cctgcacccc ctgccttctg cgacagacac tgcaaaagaa agaaaaagca
      721 tatttctccc gggaacatat gtattcggaa tctttccatc cgacacagag cccttcggaa
      781 ccttgagctg tttttcggta agtgagcttc ccaggaaaac ttccgtgtca agccgaataa
      841 ccctgtgttc cactccgagt ctatacgtaa tcgcagctgc tcttttcagc tctactctgt
      901 gtctctgtga atagtcaaaa gaaagtgcaa tcggcctttt ccttttcttc aaagcatagt
      961 acaggcagac tgttgaatcc agtccccctg aaaacagaac gactgcattt gatttatcag
     1021 cctttgacgt catcaggtga aatctccttc agaacattat tgtagttctc tgcctcagag
     1081 tccctgccga gcttggacag gatattgata gccagctgaa gccctgcacg gtctttcggc
     1141 ttttccttca gaaagttcct gatcatttcc tctgctctca caaggtcatt gagatgataa
     1201 aaaacgaagg caagatgaaa ataaagattt atataggaag ggatgatatt atatgctttc
     1261 cataacatat cctgcgcctc cgtcaggttt ctcgaccgga gaagctttat tcccgcgtta
     1321 tagacctcta tgaacctggt ccaggaggtt tcaagttcaa gaaccagcat ggaaacatca
     1381 tctcttaccg gagcgccgtc cataaatttt tccagagatc tgacacactc cgttatctgt
     1441 tccccaagac tgtcccctga agttcttttc agacattcaa tgaatctgtc cagcccgaat
     1501 tcctgcccct gcctgttctt gtgctcaaca agaccgtctg tgtaaagata aattctgtcc
     1561 ccgctgtgga gcctcatggt gctgttttca tagagatcgc ctgcttcttc aagggaaccg
     1621 atgaatatcc cccctgtatc cagaagttca tattcatttc tggaactgac ataatagact
     1681 gctcttggat gcgctgcatt gctgtaggta attctgtttt ttgaatcaat cttcagcata
     1741 aatgcagtca gatagtcgct ggtcttgact ttttcgacca gatccagatt aacttttctg
     1801 aatatttcat ccggttccaa atgctcttca atgaatgcgg aaaatgactg ttttgcggac
     1861 atagtgatca gcgccgcagg aacaccgtgg ccggaaacat cggcaaggag aacaaaaaga
     1921 taattcggga aacgaaaaag gtcataatag tctcccgata cttttcccat cggctg
//
LOCUS       DAOQGJ010000723         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39686_length_1973_cov_4.201251, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000723 DAOQGJ010000000
VERSION     DAOQGJ010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39686_length_1973_cov_4.201251"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PK683_11485"
     CDS             <1..586
                     /locus_tag="PK683_11485"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09116.1"
                     /translation="ASPTEFESASVLFTDFVGFTAIAEKMNPAELIAELDICFSFFDQ
                     VCKKYNLEKLKTIGDSYMCVGGVPIENSTHSEDAVYAALEIQRFMKEMKTERERSGRK
                     FWEIRIGIHSGPLVAGIIGSTKFSYDIWGSTVNIASRMESSGTAGRINVSEAVKKLTE
                     NKFLFEHRGRIKTKESEFEMYYVLGIRNGHLITN"
     gene            618..1103
                     /locus_tag="PK683_11490"
     CDS             618..1103
                     /locus_tag="PK683_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin family protein"
                     /protein_id="HNH09117.1"
                     /translation="MRELDALVEQKTGLSIERAMRIDEVNLYNRLGEETFWKLSRTFY
                     AKVYNDEEEKWFKNIFKNRSMEEAIQNQVEFFMQRMGGPAYFSKRKGHPALIGRHMPF
                     NMGEKAAVRWLHHMKASLEEVKEIDEDSRSRMMDYFTHTAYFLSLGVTMQQSMRGENQ
                     D"
     gene            1126..>1973
                     /locus_tag="PK683_11495"
     CDS             1126..>1973
                     /locus_tag="PK683_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNH09118.1"
                     /translation="MNSEQIKKELYKIKHPDVKKDIISIGMLLGVDVLPEESIIRLKV
                     PNSDRKLQIGLEAQIRQLISKQITGKLKIKFEVDPGFSLDDSNKVPGVKKIIAVGSGK
                     GGVGKSTVTINLAAYLASQGLKVGVMDADIYGPSIGKMTGFSGRVALQTEEDKIYPVE
                     KFGIKIISFGFLVDENQPVVWRGPMLGKAVEQFLYDVVWGELDYLLVDLPPGTGDVQL
                     SLAQLIDLSGAVLVTTPQKVATLDASKAATMFAQVKVPILGIIENMSYFIPPDAPEKK
                     YKIFGEG"
BASE COUNT          640 a          364 c          477 g          492 t
ORIGIN      
        1 agcatctcct acggaatttg aatctgcctc tgtgcttttc actgattttg tagggtttac
       61 tgccattgct gaaaaaatga atcctgcaga actgattgca gaactcgata tatgcttctc
      121 cttcttcgat caggtctgca aaaaatacaa tttggaaaaa cttaagacaa tcggcgactc
      181 ctatatgtgt gtgggcggtg ttccaattga aaacagcacc cattcagaag atgcagtgta
      241 tgcggctttg gaaatccaaa gatttatgaa ggaaatgaaa acagaaaggg aaagatccgg
      301 aagaaaattc tgggaaatcc gaatcggaat tcattccgga ccgcttgtgg caggaattat
      361 cggaagtaca aagttttcct atgacatctg gggaagcact gtcaacattg ccagcagaat
      421 ggagtcctca ggaaccgcag gcaggataaa tgtttcggaa gctgtcaaaa aactgaccga
      481 aaacaagttt ctgtttgaac acagaggccg aataaaaacg aaagaatccg agtttgagat
      541 gtattatgtt ctcggaataa gaaacggtca cctgataaca aattgaccta aaggcatact
      601 gaaaaaaacg tgaattcatg cgcgaactcg atgcacttgt agaacagaaa accggactct
      661 ctattgagag agcaatgaga attgatgagg tcaatctgta taacagactg ggtgaagaga
      721 ctttctggaa actgagcaga accttttacg caaaagttta caatgatgaa gaagagaaat
      781 ggttcaagaa tattttcaag aacagaagta tggaagaagc aatacagaat caagtggagt
      841 tttttatgca gagaatgggc ggaccggctt atttttccaa aagaaaagga catcctgcat
      901 tgatcggaag acatatgccg ttcaatatgg gagagaaagc tgcagtcaga tggcttcatc
      961 atatgaaagc ttcacttgag gaagtcaagg aaatcgacga ggattccaga tcccgaatga
     1021 tggactattt cactcatacg gcatactttt tatccctggg cgtaacaatg cagcagagta
     1081 tgagagggga aaatcaggac tgattgtgtc cttaaggaaa aaataatgaa cagtgaacag
     1141 ataaaaaaag agctttataa aatcaagcat ccggatgtga aaaaagatat catttccatt
     1201 ggaatgctcc tcggtgtaga tgttctgccc gaagaatcca ttatcagact caaggttcca
     1261 aattcagaca ggaagcttca gattggactg gaagcacaga tcagacagct gatttcaaaa
     1321 cagatcacag gaaagctcaa aatcaaattt gaagtggatc cggggttttc actggatgac
     1381 tcgaacaagg ttccaggggt aaagaaaatc attgcagtcg gatccggtaa gggcggtgta
     1441 ggaaaatcca ctgtaacgat caatcttgcc gcatatcttg cctcacaggg actgaaagtc
     1501 ggagtaatgg atgcagacat ctacggaccg tccataggca aaatgacagg attcagcgga
     1561 agggtggctc ttcagacaga agaggataaa atatatcccg ttgaaaaatt cggaatcaaa
     1621 attatctctt tcggttttct ggtagatgag aatcagccgg tagtatggag aggtcctatg
     1681 cttggaaaag ccgtggagca gtttctgtat gacgttgtct ggggcgaatt ggactatctt
     1741 cttgtggatc ttccgcctgg cacaggcgac gttcagcttt cgcttgcaca gctgattgat
     1801 ctttccggag ctgttcttgt gacaactcct cagaaggtgg ctacgctgga tgcatctaag
     1861 gctgctacaa tgttcgctca ggtgaaagtg cctatccttg gaatcattga gaatatgagc
     1921 tattttattc ctcctgatgc accggaaaaa aaatataaaa tattcggtga ggg
//
LOCUS       DAOQGJ010000724         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39709_length_1973_cov_2.626694, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000724 DAOQGJ010000000
VERSION     DAOQGJ010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39709_length_1973_cov_2.626694"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PK683_11500"
     CDS             <1..1088
                     /locus_tag="PK683_11500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH09119.1"
                     /translation="VMRQCPAEKQVMLFSATLSYYVVRLASDYLRDPVEIRIEPDKVV
                     TENIDQKIVHLGRDEKMAFLINSILEDKEEGLGIIFTNLKVMIPELVSTLRRHGIPVT
                     GISSTLDQKKRIRLLKDFKLGKYKYLIATDVASRGIDVDNIKVVYNYDLPMDTENYVH
                     RIGRTARAGKTGRSVSFCSEKDYTELEKIEKMLGSKLESIHVEETYLQMPKGEFEPFV
                     VPGERIQEFTPKDKRQKPDRKRNDRNFDKNKDRNRNNKDRKFKEKSFPVKKDLKESVD
                     EAIDLIQKADTVLGKENTKKKPDKNSKKFQNAKVHKVTEKKAEISGSKDKSYDKSKRN
                     LFDINDYRQEEPKKISLWQRLKSFFGL"
     gene            1214..1879
                     /locus_tag="PK683_11505"
     CDS             1214..1879
                     /locus_tag="PK683_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09120.1"
                     /translation="MNGEIRGNNGESILFQVNSSYYNYRGMVEKASKPVKYIHEKIFI
                     PLEIAESALKNLLENEVSYRQSRGKLLVNLYGNFKIEPEIIDLDMIIIDPGHGGKAPG
                     AMSILGDPEKNYTQIKVSNNNPDDFSYQPMMFLDFIDEEEKQKIKKEEASVKIPEKVV
                     EKVSPVSKSINVNALNNIKKYSNMTPNKYDNNSIKKVQLKHGRIPNKDEQQIHSLGFI
                     DDI"
BASE COUNT          738 a          340 c          389 g          506 t
ORIGIN      
        1 atgtaatgag gcagtgccct gcagaaaagc aggttatgct tttctccgcc accctttcct
       61 actatgtagt ccgacttgca tccgactatc tcagggatcc tgtcgaaatc agaattgaac
      121 cggacaaggt agtcacagaa aatatcgacc agaaaattgt gcatttggga agggatgaaa
      181 aaatggcctt tctgatcaat tcgattctgg aagacaagga agaaggactc ggaatcatct
      241 tcactaatct caaagtaatg attcctgaat tggtcagcac tctgagaagg cacggaatcc
      301 ctgtgacagg gatctcctcc accttggatc agaaaaaaag gattcgtctg ctgaaagact
      361 tcaagcttgg aaaatacaag tacctgattg cgactgacgt ggcatccaga gggattgacg
      421 tggacaatat caaagtagtc tacaattacg atcttccgat ggatacagaa aattatgtac
      481 atagaatagg aagaactgca agggcaggaa aaacaggaag atcagtaagt ttctgctccg
      541 aaaaggacta tactgaactg gaaaaaatag agaagatgct cggctccaag ctggaatcga
      601 tccatgtgga agaaacctat cttcaaatgc ccaaaggaga atttgagccc tttgtcgttc
      661 caggggagag aattcaggaa tttactccta aagacaaaag acagaaaccg gacagaaaga
      721 gaaatgacag gaactttgac aaaaacaaag acaggaacag gaataataag gacaggaagt
      781 tcaaggaaaa atcctttccc gtcaagaaag accttaagga atctgttgat gaagcaatag
      841 atctgataca gaaggcagac actgtccttg gaaaggaaaa tacaaagaaa aaaccggata
      901 aaaactcaaa aaaatttcag aatgcaaaag tgcataaagt tactgagaaa aaagctgaga
      961 tctctggctc aaaagacaaa tcatatgata aatctaaaag aaatcttttc gatatcaatg
     1021 attaccgaca ggaagaacct aagaagattt ctctgtggca aagattaaag tctttttttg
     1081 gactctgata atttccttat ctttttcggt atcctccgaa gaagagatac cgcttctgaa
     1141 aggttcttat gtcgactttg atgacctgaa ggcaaaaact tcaggtctca gtgtttccat
     1201 gaaaggaccg acgatgaacg gtgaaatcag agggaacaat ggggagtcca ttctttttca
     1261 ggtcaattct tcctactaca attacagggg aatggtggaa aaagcatcca aacctgtaaa
     1321 atatatacat gaaaaaatat tcattccgct ggaaatagcc gagtctgcac tgaagaatct
     1381 tctggaaaat gaggtctcct acagacagtc tcgcggaaag cttctggtaa atctgtatgg
     1441 aaatttcaaa atagagcctg agatcattga tctggatatg atcatcattg atcccggtca
     1501 cggaggcaag gccccgggag ccatgtccat tctgggagat cctgaaaaaa attatactca
     1561 aataaaagta tctaataata atcctgatga tttttcttat caaccaatga tgtttttaga
     1621 ttttattgat gaagaagaaa aacaaaaaat aaaaaaagaa gaagcttcgg ttaaaatacc
     1681 agaaaaggtt gttgaaaaag tatcaccagt atctaaatct attaatgtaa atgctttaaa
     1741 taacataaaa aaatactcta atatgactcc aaataaatat gataataact ctataaaaaa
     1801 agtacaacta aaacatggaa gaatacctaa taaagatgaa caacaaattc attcattagg
     1861 ttttattgat gatatctaaa ataataatag catggagctt ttaatgaaaa aatttttacc
     1921 tattttttta gctagtttat tgattatttc taattttcaa tcttatgcta aag
//
LOCUS       DAOQGJ010000725         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39745_length_1972_cov_2.721440, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000725 DAOQGJ010000000
VERSION     DAOQGJ010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39745_length_1972_cov_2.721440"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PK683_11510"
     CDS             <1..442
                     /locus_tag="PK683_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09121.1"
                     /translation="KVFHKENFQDTATFLRKQMENGSISEIIPYLFSCIQGQRICYHC
                     DEFGMTSSESKLRLIAVQRDKIDFYLTKINTPEKTIEFFPKKNSEKGLYEKYMGRTDD
                     YMQEVPFLKFTFLKNAKQDEDWKIQDIDGRILILNPVKFCYFGA"
     gene            complement(444..767)
                     /locus_tag="PK683_11515"
     CDS             complement(444..767)
                     /locus_tag="PK683_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09122.1"
                     /translation="MKKIFLLLLLYSSAEAEQICTSANFQKFCTGPNVPEFRIDTSYI
                     HYSLDCTKVFLWYPRKKFGEENCYVCADKTNEKIIGTLMKSIRRENRNCGRIIEISNK
                     ELPYR"
     gene            complement(764..1546)
                     /locus_tag="PK683_11520"
     CDS             complement(764..1546)
                     /locus_tag="PK683_11520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH09123.1"
                     /translation="MKEAVLITGASAGIGYDLAELFAQEKRDLVIAARNEKKLTEIAA
                     EFQKKYGTEVHVLGIDLSVPGSSKKVIEFTKSKGLFIETLVNNAGFGTNGEFAKNSMK
                     DEMEMLQLNITSLAELTKLFLEDMISNKKGHILNVASTAAFQPGPYMSGYYASKAFVL
                     HFSEGLSEEVRDYGVSVSILCPGPVKTQFQERAGMHDAALVKGPLLVDSRDVAKTAYK
                     GLKKGKLIIIPGFMNWLLVQSVRFSPRFAVRKIAKYLNKVPA"
     gene            complement(1610..>1972)
                     /locus_tag="PK683_11525"
     CDS             complement(1610..>1972)
                     /locus_tag="PK683_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09124.1"
                     /translation="KNKSSANQGKDDKNKSSANQGKDDKNKSSSDQGKDDKNKSSADQ
                     GKDDRNKENSGTEQNKNTVSGITDEENRLIKKELERQDKNKDLKNTKYHKSRHQQNPK
                     KNWGSTDELRESLRKTLW"
BASE COUNT          525 a          434 c          375 g          638 t
ORIGIN      
        1 gaaagtattt cataaagaaa attttcaaga tactgctaca ttccttagaa aacagatgga
       61 aaacggaagc atttctgaaa tcattcctta tctgttcagc tgtattcagg gacagagaat
      121 ctgctatcac tgtgacgaat tcggaatgac aagttcagag agcaaactca ggctgattgc
      181 agtgcaaaga gataaaatcg acttttacct aacgaaaatc aatactcctg aaaaaaccat
      241 tgaattcttt ccgaagaaaa attctgaaaa ggggctttac gaaaaatata tggggcgtac
      301 ggacgattat atgcaggaag ttcccttttt aaaattcaca tttttgaaaa atgcaaagca
      361 ggatgaggat tggaaaattc aagacatcga cggaagaata ctcatcctga atccagtcaa
      421 gttctgctat ttcggagctt agtttatctg tagggaagct ccttgttcga aatctcaatg
      481 attcttccgc agtttctgtt ttccctgcgg atgcttttca taagtgttcc tatgattttc
      541 tcattggttt tgtccgcaca gacgtaacag ttttcttcac cgaatttttt tctgggatac
      601 cagagaaaca ctttcgtgca gtcaagtgaa taatggatat aggatgtatc tatcctgaat
      661 tcaggcacat tcggcccggt gcagaatttc tgaaaatttg cagatgtgca gatctgctct
      721 gcctctgcag aagaatacag aagcagaagc agaaatattt ttttcacgca ggaaccttgt
      781 tcagatactt tgcaatcttt ctcacggcaa atcggggtga aaaacggaca gactgtacta
      841 gaagccaatt cataaatcca gggataataa tgagttttcc ttttttcagt cccttgtatg
      901 cagtctttgc gacatccctg ctgtctacaa gaaggggtcc ctttaccagc gctgcatcgt
      961 gcatccctgc tctttcctga aactgagtct tgacaggccc cgggcacaga atgctgacac
     1021 tgactccata atctctgact tcctcggaaa gcccctccga aaaatgcaga acaaatgcct
     1081 tggaggcgta atatccgctc atatagggac cgggctgaaa tgctgcagtg gaagccacat
     1141 tcagaatatg tcctttttta tttgagatca tatcctccag aaacagtttt gtcagctccg
     1201 cgagagatgt aatgttcagc tgaagcatct ccatctcgtc tttcatggag ttttttgcaa
     1261 attcgccgtt tgttccgaat cctgcattat tgaccagagt ctcaatgaaa agacctttgg
     1321 attttgtaaa ctcgattact tttttagaac ttcccggaac agaaagatct atgccgagta
     1381 catgaacttc cgttccgtat ttcttctgaa attcggctgc aatctcagtc agtttttttt
     1441 catttcttgc cgcaatgaca agatctcttt tttcctgtgc aaacagttcc gcaagatcgt
     1501 accctatccc ggcagatgca cccgtaatca gaacagcttc tttcattttt ccctcactca
     1561 tcgtaggttt tcaactgcag acagaggagc aagaaaaaaa ttatggatac taccagagtg
     1621 tctttctgag agattcccga agttcgtctg tagaacccca gttcttttta ggattctgct
     1681 gatgtctgct tttgtgatat tttgtattct tcagatcctt attcttatcc tgtctctcca
     1741 gttctttttt aatcagtctg ttttcttcat ccgtaattcc tgaaacagta tttttgttct
     1801 gttctgtccc tgaattctct ttgttcctgt cgtcctttcc ctgatctgca gaagacttat
     1861 tcttatcatc ctttccttgg tctgaagagg atttgttctt atcatccttt ccctgattcg
     1921 cagaagactt gttcttatca tcctttccct gattcgcaga agacttgttc tt
//
LOCUS       DAOQGJ010000726         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39796_length_1971_cov_2.005741, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000726 DAOQGJ010000000
VERSION     DAOQGJ010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39796_length_1971_cov_2.005741"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1274
                     /locus_tag="PK683_11530"
     CDS             177..1274
                     /locus_tag="PK683_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09125.1"
                     /translation="MKPFILLFFLTFVFSVYSQETLSEEGPGSGLDRKRNFYVQLGKA
                     EWKSGSLGNMYSVQRSRAVNTLLGLPFDSSSFPVHGGTPSYLDAKALTFGVRLGSSAR
                     HRANLMLRYVTTDDLKYSTAAREQANIDLLFLRVQSFENRIEMTKANPLKRGLFQYDH
                     DIYLLPDHSSKFLQGLGIKVGFALQGDNVKGNTTPYGNVSSVTAPSLFSFSSGSTSTN
                     SFIYPIPGKFDQTDYSGYVILGLSYRLALAKNQELDFSYSILNGKANGSSVQTSYALF
                     NGNLPYQVGTKTTYEGAVTGNAVNLGYSYFFNEKFFVRAGYETRDLSYKLEHLKTKAV
                     SGTSSPYRDYGPYPTYKDSIKYYTVEFAYRF"
     gene            complement(1365..1859)
                     /locus_tag="PK683_11535"
     CDS             complement(1365..1859)
                     /locus_tag="PK683_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09126.1"
                     /translation="MHSVERKIRIDSPLPVVLDIMLDWNSYNRFLPSIDSFRVLSHDR
                     KECRVETETVARLPVFGTVVKTAQTIIYDLETLTFQTVDRSGFFKEFKGFRKLEPDGD
                     SVIFYSRVDYVYSSSFLDFLNQNIRNLIEKNVTSLQDSMKLEAEKRRSEKLTLADLKI
                     KYGE"
BASE COUNT          542 a          407 c          424 g          598 t
ORIGIN      
        1 tggttcttat acatttccag caatcaatac ggcatataat attttaactt atgttggtgg
       61 tccaactaaa tcaggttcta ttagagatat tcaaataaaa agagatggaa aaatacttga
      121 cagtcttttc tgaaaaattc atataaaatc tgcaaattat actgtaaggg tgaaaaatga
      181 agccgtttat tcttttattt tttttgactt ttgttttttc tgtttattct caggaaactc
      241 tttctgaaga aggtcccggg tcaggacttg acaggaaaag gaacttttat gttcagctgg
      301 gcaaggcaga atggaaatca ggttccttag gcaatatgta cagtgtacag agatcgagag
      361 cagtcaatac tctgcttggc ctgccttttg attcttccag ttttcctgtt catggaggaa
      421 ctccttccta tctggatgca aaggctttga cgttcggagt aagactgggc tcttctgcca
      481 gacacagagc aaatctgatg ctgagatatg tgactacgga tgatctgaaa tacagcacgg
      541 ctgcccggga gcaggcaaac atagatctgc tttttttaag ggttcagagt tttgaaaaca
      601 ggattgagat gaccaaggca aatccgctga agagaggact tttccagtat gatcatgata
      661 tttaccttct tccagatcat tcttccaagt ttctgcaggg gctcgggatc aaggttgggt
      721 ttgcacttca gggagacaat gtaaaaggga atacaactcc ttacggcaat gtctcatctg
      781 ttactgcacc ttctcttttc agtttcagtt caggttccac ttccacaaac tctttcattt
      841 accctatccc cggtaaattt gatcagactg actattccgg atatgtaata ctcgggctgt
      901 cttaccgtct tgcgcttgcc aagaatcagg agctggattt ttcctactcc atactgaacg
      961 ggaaggcgaa cggatcttct gtgcagacgt cctacgcgct ctttaacggt aatcttccat
     1021 atcaggtcgg aactaagacc acatatgagg gagccgtaac agggaatgcg gtcaatctgg
     1081 gatacagtta tttcttcaat gaaaagtttt ttgtccgtgc cggctatgaa accagggatc
     1141 tgagctataa gcttgagcat ctgaaaacca aggctgtttc aggaacatct tctccataca
     1201 gggactatgg cccttatcca acttacaaag attcgataaa atattatact gtagaatttg
     1261 cttatcgatt ctgaaaaatg actgccggtt tcatcagatc ggcagtatgt cttttcggcg
     1321 ggcggcggtt tcactgaagc tcgggactgt cggcaccgga aaaattattc tccgtacttt
     1381 attttcagat cggccagagt gagtttttca gatcttcttt tttcggcttc cagtttcata
     1441 ctgtcctgaa gggatgtcac atttttttca atcaggtttc ttatgttctg attcagaaaa
     1501 tcgagaaatg aagatgagta aacataatcc accctggaat aaaaaatcac agagtctccg
     1561 tcaggttcca gttttctgaa ccctttgaat tccttgaaaa atccggacct gtcgacagtc
     1621 tgaaatgtga gagtttccag atcataaatg atcgtctgag cagtttttac cactgttccg
     1681 aatacaggaa ggcgggcgac tgtttctgtc tccactctgc attcctttct gtcatgggaa
     1741 agaaccctga aggaatcaat cgaaggaaga aaccgattat aggaattcca gtcgagcata
     1801 atgtcgagca cgacaggaag aggggagtca atccggatct ttctttccac agaatgcatc
     1861 agatgttttc ctgaagccgg tatctgtttg ctcttctgaa agttccgaaa tcattaaaac
     1921 gtacaccgta ctttttcatt gcaccgtgag cataggaaga aatcatctgt c
//
LOCUS       DAOQGJ010000727         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39845_length_1969_cov_2.845350, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000727 DAOQGJ010000000
VERSION     DAOQGJ010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39845_length_1969_cov_2.845350"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /locus_tag="PK683_11540"
     CDS             complement(<1..1937)
                     /locus_tag="PK683_11540"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH09127.1"
                     /translation="MKKGTAFVRNIRDFMILYLLVCTVGGFVSCARTVAAPFSSPSAV
                     NGVLDLRDWDFEKKGIVNLAGQWNFYWNRFLSFEETAKNERNGFLNVPGSWKGMTVGQ
                     RVLRGDGYCTIHLLVRLPAELRSRLSIRIPVIGTAHRVWINEQLAFEAGKVGKTREEM
                     VPYLFPHIYSPELGSRNEIHITMQISNFMDTKGGVWEHFFLGTDQDINEQKFITLSSE
                     MMVFGGLFIMAVYHFGLFFIRKKDRSTLFFGLFCLVYAVRTLITEQRVLMSVFPSLPF
                     DITYRAEYLTAFLNLPFFVSFSYYMFPDKINCAYLKITWIFFSVLCAAVLILPHFEYT
                     HLLIVFEIYALLSIPYLMHSYISSVISRSEGAVTSLAGIIILAIGAVLDIMHNEHLNF
                     LHYITPFTLFGFLFFQSYSLAAKFSKAFLVSENLAAELTVQSSTLKTVNEELLSLQKG
                     LEEKVLQRGEELEISYRKNLLEIQKVNELERKISAQTVRQDLFVDIHDNLGAKLLNLS
                     IQLNQLEPDIDAKSTIQDSVSDIMKSLRNHLLAFEDIALIEENFRTGFKNFLVRRYSV
                     SGRKIELNSDPDFRLEKIPEKCYHHLFSILQELISNDLKYGKGITRWTVSQDSRCSVF
                     QMTADSVYETSAYAKGRGHKTV"
BASE COUNT          607 a          452 c          390 g          520 t
ORIGIN      
        1 acagttttgt gtcccctgcc ctttgcgtaa gcactggttt catagacaga atctgcagtc
       61 atctgaaaca cggaacaccg gctgtcctga ctgaccgtcc atcgggtaat acctttgccg
      121 tatttcaagt cattgctgat cagttcctga agtatgctga acagatgatg gtagcatttc
      181 tcaggaattt tttccagccg aaaatccgga tcagaattca gttcaatttt ccggcctgaa
      241 acactgtatc tgcggacaag aaagttctta aagcctgtac ggaaattttc ttcaatcaat
      301 gcaatatctt caaacgcaag aagatggttc cgaagacttt tcataatatc acttacagaa
      361 tcctgaattg tactctttgc atcaatatcc ggttccagct gattgagctg aatggaaaga
      421 ttcagaagtt tggctcccag attgtcatga atatctacaa acagatcctg tctcaccgtc
      481 tgagccgata tctttctttc cagttcattc actttctgga tttccagaag gtttttccga
      541 taactgattt ccagctcttc cccgcgctgc aggaccttct cttccaggcc cttctgcaga
      601 ctcagcagtt cttcattgac tgtctttaat gtgctgctct ggacagtaag ctcagcagcc
      661 agattttcag aaacaagaaa tgccttggaa aattttgcag ccagagaata cgactgaaag
      721 aaaagaaatc caaaaagagt aaaaggagtt atgtaatgca gaaaattgag atgttcgtta
      781 tgcataatgt ccagaacagc accaatggca agaataataa tcccggcaag cgaagtgact
      841 gccccttctg accggcttat gactgaagaa atatatgagt gcataagata ggggatggac
      901 agcagtgcat aaatctcaaa tactatcaga agatgagtat attcgaaatg aggcagaatc
      961 agaacagccg cacagagaac agaaaaaaat atccatgtga tctttaaata tgcacagtta
     1021 attttatccg gaaacatata ataggaaaaa gagacaaaaa aaggaagatt aagaaaagct
     1081 gtcagatatt cagccctgta agtgatgtca aacggaagtg aagggaatac ggacatcaga
     1141 accctctgct cagtgatcag tgttcggacg gcgtaaacca gacagaacag tccgaaaaaa
     1201 agagtgctcc tgtccttttt tctgatgaaa aacaggccga aatgatagac tgccataata
     1261 aaaaggcctc cgaaaaccat catctccgaa gacaatgtaa tgaatttctg ttcatttata
     1321 tcctgatctg tccccaggaa aaaatgctcc cagacccctc ccttggtgtc cataaaattg
     1381 gaaatctgca tagtaatatg aatttcattt ctgctcccga gttcaggact gtatatatga
     1441 ggaaaaagat aaggaaccat ttcttctctg gtcttcccga cttttcccgc ctcaaaagca
     1501 agctgctcat tgatccagac tctgtgtgca gttccgatca cgggaatgcg aattgagagt
     1561 ctgcttctta attctgccgg aagcctgacc agaagatgaa tcgtgcagta gccgtctccc
     1621 cgaagcaccc tctgtccaac cgtcattccc ttccagcttc cgggaacgtt cagaaatccg
     1681 ttcctttcat tttttgcagt ttcttcaaaa ctcaggaacc ggttccaata aaaattccat
     1741 tgaccggcca aatttacaat tcctttcttt tcaaaatccc agtcccgcag atccagaaca
     1801 ccgttcacag cagaaggact ggaaaaagga gcagcgacag ttctggcgca ggagacaaat
     1861 ccgccgactg tacagacaag aagataaaga atcataaagt ctctaatatt tctcacgaag
     1921 gccgttccct ttttcaatgc tgaattcctt ctgcatatct caggaagct
//
LOCUS       DAOQGJ010000728         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39925_length_1967_cov_2.678347, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000728 DAOQGJ010000000
VERSION     DAOQGJ010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39925_length_1967_cov_2.678347"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1967)
                     /gene="greA"
                     /locus_tag="PK683_11545"
     CDS             complement(<1..>1967)
                     /gene="greA"
                     /locus_tag="PK683_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNH09128.1"
                     /translation="NPEIAKKYAMSIIDEDKPKAISFLKQAAESFARSKDYQNLEEIW
                     PILISNNFEDLLFFERIERILLANRERTRLVVLLFPLMETYKNLEDYDKTIHFLKKIL
                     DNEPLSPKARNELIRAYKSKYAGHSLLDEFLKMSELGNTKKPIKACITNFERNIVFDT
                     NNYVMHRNWGVGKIKSISSESDSIVVDFVGKPDHKLSIQMAITSLKPLKKDHIWVKLY
                     ETPNEIHRMFQDDVSNFIAELLTSHDNTMTLNDIKSEIIGRFVKKTEDWTKWWNKAKL
                     ALKKDPRIGFNPKKKDEIVFRQKPISLTEELTEKFNAQTDINKKLDIALEALEVYHEA
                     EGAVESFNHFYYEEEEAKDTFRRIIAYIYMEIASGIVEKDDLPRHMSEAEAGRLFSAI
                     SKEEAIQFSKQMSNLEVKKVYVNLIRKFHPAWTDIFVGILFEVPVKINKYVFSLLVQD
                     AKFSELNLFLETVMNRMKDFPEIFLWTAKSQLSGLWSYTWMNLNENEMVLRVFRLLKP
                     LSKIEEKGTKLKNMASEILFGNKNEVISKTIHSAGDEFLRRLYALYKEVPFISDSEKE
                     KFLELISHLKPGFEWNQALASEEKEEEEDVLANIPANVVLVTEKGYSKKKEAFDHLVN
                     VEMPENSRDIGEAQEKGDLRENAEYKAAMEK"
BASE COUNT          542 a          414 c          332 g          679 t
ORIGIN      
        1 gtttttccat tgcagccttg tattcagcat tctccctcaa gtcgcccttt tcctgagcct
       61 caccgatatc tctagagttt tcaggcattt cgacattgac gagatgatca aatgcttctt
      121 ttttcttgct gtaccctttt tctgttacaa gaactacatt tgccggaata tttgcgagaa
      181 catcctcttc ttcttctttt tcctcagaag cgagagcctg attccattcg aaacctggct
      241 taagatgaga gatcagttca agaaattttt ccttttctga atccgaaata aatggaactt
      301 ccttatacag cgcatagaga cgtcttaaaa actcatcacc tgcagaatga attgttttgg
      361 aaatcacttc atttttattt ccaaacagta tctctgaagc catattcttc agtttagttc
      421 ccttctcttc tatctttgaa agaggcttca aaagtcggaa tactctcaga accatttcat
      481 tttcattgag gttcatccaa gtgtaactcc agaggccgga aagctgtgat tttgcagtcc
      541 agaggaaaat ctcgggaaag tctttcattc tgttcatcac agtttcaagg aaaagattca
      601 gttcagagaa ctttgcatcc tgaaccaaaa gcgagaatac atacttattg atttttactg
      661 ggacttcaaa aagaattcct acaaaaatat ctgtccaggc aggatggaat ttacgtatca
      721 aatttacata gacttttttg acttcgaggt tgctcatctg cttggaaaac tggattgcct
      781 cttctttgga aatcgcgctg aaaagtcttc ctgcctctgc ttcagacata tgtctaggaa
      841 gatcatcttt ttcaacgatc cctgaagcaa tctccatata aatataagca atgattctgc
      901 ggaaagtgtc tttcgcttcc tcttcttcat aataaaaatg attgaaggat tcgactgccc
      961 cttctgcttc atggtagact tccaaagctt ccagtgctat atccagtttc ttattgatgt
     1021 cagtctgagc attgaacttt tcggtcaatt cttcagtcag tgaaataggc ttttgacgga
     1081 atacaatttc atctttcttc tttgggttaa aaccgattct aggatctttt ttaagtgcaa
     1141 gttttgcctt attccaccat tttgtccagt cttctgtttt ctttacaaac cttccaatga
     1201 tttctgattt gatatcattc aaggtcattg tattatcatg agaagtcaga agttccgcaa
     1261 taaaattcga aacatcatcc tgaaacattc tatgaatttc atttggagtc tcataaagct
     1321 tcacccagat atggtctttt ttgagaggtt tcagacttgt aattgccatc tgaatagaaa
     1381 gcttgtgatc cggctttcct acaaagtcta caacaatgga atcgctctca ctgctgatcg
     1441 atttgatctt gcctacaccc caatttctgt gcatcacata attgttcgta tcgaaaacga
     1501 tattcctttc aaagttggta atacatgctt tgataggctt tttagtattt cccagttcag
     1561 acattttcag gaattcatcc agcagagaat gccccgcgta ctttgattta taagcacgaa
     1621 tcagttcgtt tctggccttt ggagacaaag gctcattgtc caaaattttc ttaagaaaat
     1681 gaattgtttt gtcatagtct tctaaattct tatatgtctc cattaatgga aacagaagca
     1741 cgaccagtct tgttctttca cggttagcca gaagaattct ttcaattctt tcaaaaaaca
     1801 aaagatcttc aaagttattg gaaatcagta ttggccagat ttcttccaaa ttctgataat
     1861 ctttggagcg cgcaaaagat tcagctgcct gcttgaggaa actgatcgct ttaggcttat
     1921 cctcatcaat gatggacatc gcatattttt ttgcaatttc aggattc
//
LOCUS       DAOQGJ010000729         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_39956_length_1966_cov_2.952904, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000729 DAOQGJ010000000
VERSION     DAOQGJ010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39956_length_1966_cov_2.952904"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PK683_11550"
     CDS             complement(<1..548)
                     /locus_tag="PK683_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09129.1"
                     /translation="MQTKIELFLLGTTLAVNALVILTALFRKPNSSARSTFIIFSFLF
                     FFWNCFEVMHRFFPIEPLWLKIFQLGFSQATLYALVVFSLNFPYFGRRSTKLIYFTAF
                     LGGVGYIILMMTFAWPFVEIRFPLDFQFFAKFSQLLSRAYSVLCVCAFLVISFSKLTQ
                     SVAKLKRIFYRVLGFTSLITVLV"
     gene            712..1932
                     /locus_tag="PK683_11555"
                     /pseudo
     CDS             712..1932
                     /locus_tag="PK683_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
BASE COUNT          644 a          380 c          458 g          484 t
ORIGIN      
        1 accaggacgg tgatcaggga agtgaatccc agaactctgt aaaagattct tttgagtttc
       61 gcaactgact gagtcagttt ggagaaagag atgaccagaa aagcacagac acagagaaca
      121 gaatatgccc tcgaaagaag ctgtgaaaat ttcgcaaaaa actgaaaatc gagtggaaaa
      181 cgaatctcca cgaacggcca ggcaaaggtc atcatcagaa taatgtaacc tactccgccc
      241 agaaatgcag taaaataaat cagcttagtg gatctgcgtc cgaaataggg aaaattcaga
      301 gaaaaaacta ctagagcata aagtgttgcc tgagaaaacc caagctgaaa aattttcagc
      361 caaagaggct caattggaaa aaaacggtgc attacttcaa aacagttcca aaaaaagaag
      421 agaaaactga atataataaa tgtggaacgg gcagaactgt tcggtttgcg aaagagtgcc
      481 gtcaggatca cgagggcatt gactgcaagt gtagtgccta atagaaaaag ttcaattttc
      541 gtctgcataa aaaatatctt ttgcctgatt tactggaatg attgaccctt atatcggcac
      601 aacataggat gtttttattg aataggtttt tattgtcaac tcacactttc ttacgggaga
      661 aaaaatatct tgaaacccct tttctttttt agttttcttc ttttttcggg cattctgcac
      721 gcctcggaaa ttcagcctgc acaggatttc gatacaatcc gaaaaggagt cgttcagatt
      781 aaagtttatt ctcagggaat agacccttac tctccctggc agatgactcc tcttcgtgca
      841 agcacaggaa caggatttct gatagggaaa actcagattc tgacaaatgc acatgtcatt
      901 tcaaatgcaa agtacattca ggttcagaga cacaatcaga ctgtctggta tgaagtggaa
      961 attcagcatg tagcgcatga ctgcgacttg gctgtactga aagcaaaaaa ccctgaattc
     1021 tacaatgatt ctcatgtttt tgatctggga gggatacctg atctgaactc cccgattatg
     1081 gtcgtaggat atccaatagg cggggacaag atttccgtaa gcagaggaat tgtatcccga
     1141 aaagaacagt ccacatatgc tcattcacag gtggacagtc atctggtcat acaggtggac
     1201 gctgcaatca atccgggaaa ttcaggcgga ccggccatac agggaaacaa ggtggtagga
     1261 gttgcgtttc aagtggcggc aagaggagaa aacatcggtt atctgattcc cacgactgtc
     1321 atcaaatact ttctgacgga tattcaggac ggcagatacg acggatatgt ggaactcggc
     1381 acaagaacaa tgaattcatt caatacaagt ctgagaaaat acagaggaat tcctgacagt
     1441 ctggacggtg tttttgtgac aaaggtactg cggggcggaa gcgcggaagg atatctgcag
     1501 acaaacgatc ttcttctgga aattgacgga cttccgatag gaagaaacgg aactgtcctt
     1561 ctcgacaagg atgcacgagt ggactttgtg gaagttgtag acaataagca ctcaggagag
     1621 cagatccgat ttaaagtcta tcgggacgat aaacagatcg acgtcacatt ccctgcaaaa
     1681 aagatgaagc agattgaatt tatgcgtcat aaatacgatg aatccttccc ctactacatt
     1741 ttcggaggaa tggtctttca gccggtttca agagacatcc tgcaggaatg gagcagacac
     1801 actgagaccc agggcggaag ccagtttctt tacaggttct tttattttat ggaagatgat
     1861 ctgggtgaag gaaaatcaga tgaggatatt gtattctaca gaagactgaa tcattcagta
     1921 aatgcaggag catcatctcg ctgtgggatt aaatgataaa agccat
//
LOCUS       DAOQGJ010000730         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40039_length_1963_cov_3.695493, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000730 DAOQGJ010000000
VERSION     DAOQGJ010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40039_length_1963_cov_3.695493"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PK683_11560"
     CDS             <1..956
                     /locus_tag="PK683_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH09130.1"
                     /translation="EDGGLGADYLYNVIIMEESAKVGNTGFFISLHNDINVPYLAEYA
                     SPEQKKRWLPGCIDGTKITAIAMTEPNAGSDLKSIRTTAVEKGDHFVVNGQKTFISNG
                     QLADYVITAVKTSDEAISLIMIEDGMPGFERGRMLEKIGLKAQDTSELHFTDVKVPKE
                     NLIGKKGQGFRYLMTKLVSERLSLGVSAVEASATVLELTLKYIKEREAFGKKIGSFQH
                     IKFSMAEMKTEIEVCRAFADQAVMAFMKGELTTAEASMIKLHATEMQKRITDRCLQFF
                     GGYGYMMEYPIARAYLDARIQTIYAGTSEIMKEIIGRSLGL"
     gene            972..>1963
                     /locus_tag="PK683_11565"
     CDS             972..>1963
                     /locus_tag="PK683_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNH09131.1"
                     /translation="MKQKSLNYHSSFPQGKSSVQPTKSTLNSEDLSMAYSPGVAFPCL
                     EIAENPDTVYDYTNKGNLVAVISNGTAVLGLGDLGAAAGKPVMEGKGVLFKKFAGIDV
                     FDIELNSKDPAEIIQAVKIMEPTFGGINLEDIKAPECFEIEDELIRQMNIPVFHDDQH
                     GTAIITAAAVINALHISGKKPENVRVVICGAGAAAVSIGRLITHIGIKNENIYMTDSK
                     GVLNFKRKELTGVKKEFSRNTDAEQLGDILKGADFFIGVSVKDQMSKDMVASMAPNPC
                     ILAMANPDPEIPYETAKSVRNDIIMGTGRSDYPNQVNNVLGFPYIFRGALDTRAR"
BASE COUNT          617 a          358 c          475 g          513 t
ORIGIN      
        1 aagaagacgg tgggcttgga gccgattatc tgtataatgt cattattatg gaggaatccg
       61 caaaggtagg aaataccggc tttttcattt ctcttcacaa cgatatcaat gtgccttatc
      121 tggcagaata tgcttctcct gaacagaaaa aaagatggct tccaggatgt attgacggca
      181 ctaaaattac tgcaattgca atgactgaac cgaatgcagg ctctgatctc aaatctatcc
      241 gaacaacggc tgtcgaaaaa ggtgatcatt ttgtcgtaaa tggacagaag acgttcatca
      301 gcaatggaca gctggctgat tatgtcatca cggcagtcaa gacctctgat gaggcaatca
      361 gtctgattat gattgaggat ggaatgccag gcttcgaaag aggcagaatg cttgaaaaaa
      421 tcggtctcaa ggcacaggat acatctgagc ttcatttcac ggatgtgaaa gtacctaagg
      481 aaaatctgat cggaaaaaaa ggacagggct tccggtatct gatgacaaaa cttgtcagtg
      541 aaaggctttc tctcggtgta tctgcagtgg aagcctcggc caccgttctt gaactgactc
      601 tgaaatatat caaagaaaga gaagcattcg gaaagaagat aggttctttt cagcatatta
      661 agttcagtat ggcagaaatg aagaccgaaa ttgaggtgtg cagggctttt gctgatcagg
      721 ctgttatggc atttatgaag ggtgaactga caactgctga agcatccatg atcaagcttc
      781 atgcgactga aatgcagaaa cgcataaccg acagatgcct tcagtttttt ggaggatacg
      841 ggtatatgat ggagtatccg atcgccagag cgtatctgga tgcacgaatt cagacgattt
      901 acgcaggcac atctgaaatt atgaaagaaa tcatcggaag aagtcttgga ttatagaata
      961 gggaattaaa attgaaacaa aaatcattaa attatcattc atcctttccg caggggaaat
     1021 cttcagttca accgacaaag tcaacactta attctgaaga tctttctatg gcatattctc
     1081 caggagttgc ttttccgtgt ctggaaattg cagagaaccc tgatactgta tatgattata
     1141 caaataaggg aaacttggtg gcagtcattt cgaacggaac tgcagttctc ggtctcggtg
     1201 atcttggtgc agcggcagga aaacctgtca tggaaggaaa gggagttctg tttaagaaat
     1261 tcgcgggaat tgatgttttt gacattgaac tgaattccaa agatcctgca gaaattatcc
     1321 aggcagtgaa aattatggaa cctacattcg gcggaatcaa tctcgaagac atcaaggcac
     1381 ctgagtgttt tgagatagag gatgagctga ttcggcagat gaacattcct gtttttcatg
     1441 atgatcagca tggaactgca attatcacag cagcagcagt catcaatgct cttcatattt
     1501 ccggaaaaaa gcctgagaat gtgagagtag tcatctgcgg agcaggggca gcggcagtat
     1561 ccatcggaag actcattact catataggca ttaagaatga aaatatctat atgaccgact
     1621 ccaaaggtgt tctgaatttt aaaagaaagg aattgaccgg agtcaaaaaa gaattttcca
     1681 gaaatacgga tgcagaacag ctgggcgata tcctgaaggg agcagatttt tttattggag
     1741 tatctgtaaa ggatcagatg agcaaggata tggtggcatc tatggcaccg aatccctgca
     1801 tacttgcgat ggcaaatcca gatccggaaa ttccctatga aacggccaag tcggtgagaa
     1861 atgatattat tatgggtacg ggcagaagcg attatccgaa tcaggtaaac aatgttctag
     1921 gttttcctta tattttcaga ggtgctttgg ataccagggc ccg
//
LOCUS       DAOQGJ010000731         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40047_length_1963_cov_3.034591, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000731 DAOQGJ010000000
VERSION     DAOQGJ010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40047_length_1963_cov_3.034591"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PK683_11570"
     CDS             <1..1048
                     /locus_tag="PK683_11570"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNH09132.1"
                     /translation="PPGIPDEMYIIEQTGNIRLLKNKTLQKEPVFSVSGKLSKSFFSY
                     SEKGLLGIAFHPEYVKNRKLYLYYSTSSEKKGSEHKSRISEFIMKNSGSEILNTEKII
                     MEIEQPESNHNGGSILFGKDGFLYIGTGDGGGAGDRHGKTGNSQDLSNLLGKILRIDV
                     NSGNPYSVPESNPVIQGKKSEIYAYGLRNPWKVSMDRVSGEIFIGDVGQDKYEEIDIL
                     RKGANYGWRIKEGNHCFEKNCSDSGLTPPIHEYGREKGISVIGGYAYRADKKSRQYGW
                     YIFGDWIGKIYSLRKNGSVWENETLEQKNPPAEFKHINSFAQDSSGDVYILGQKDVGV
                     GNKGYIYKIRFAEK"
     gene            1195..>1963
                     /locus_tag="PK683_11575"
     CDS             1195..>1963
                     /locus_tag="PK683_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09133.1"
                     /translation="MIRIEFENDKTVETEAGKSILQISLENEIPHFHACGGHARCSTC
                     RIAVLKGLENLPGRNEKEKKLAKAKGFEDEIRLACQTVPSSDIKIRRLVNDTEDMQSA
                     VAEHGNTSGREAKLAVLFSDIRGYTSFSEKALPYDAVHILNRYFARMGDSVRANRGFI
                     DKYIGDGMMVLFGLNGETPYMAASAAVRTAVSMVNSLNELNIYLKKNFDTKFQIGIGI
                     HYGNLIVGEIGHSSKRQFTAIGDSVNFASRIESYTKQA"
BASE COUNT          681 a          360 c          455 g          467 t
ORIGIN      
        1 gcctcccgga atccccgatg aaatgtatat tattgaacag acaggaaata taagactgct
       61 gaaaaacaag actctccaaa aagaaccggt cttttcagtt tcaggaaaac tctccaagtc
      121 ctttttttcc tattcggaaa agggacttct gggaattgca ttccatcctg aatatgtaaa
      181 aaacagaaag ttataccttt attacagcac atcttcagag aaaaaaggtt ccgagcataa
      241 atcaagaata tccgagttca taatgaaaaa ttccggttct gagatattaa atactgaaaa
      301 aatcattatg gagatagaac agcctgaatc gaatcataac gggggaagca ttcttttcgg
      361 gaaggatggg tttctttaca taggaacagg cgacggaggc ggtgcgggag acagacacgg
      421 taaaacggga aactctcagg atctgtcaaa tctgctcggg aaaattttaa gaattgatgt
      481 gaacagcgga aatccgtatt ccgttccgga aagcaatcca gtcattcagg gcaaaaagtc
      541 ggagatctat gcttatggac tcagaaatcc ctggaaggtc agtatggaca gggtgagcgg
      601 agaaatattc atcggtgacg tcggtcagga taaatatgaa gaaattgaca tccttcggaa
      661 aggagcaaac tacggatgga gaatcaaaga gggaaatcac tgcttcgaaa aaaactgttc
      721 cgattcagga ctcactcccc ccattcatga atatggaagg gaaaaaggaa tctcagtcat
      781 aggaggatat gcataccgag ccgataaaaa atccagacag tacggatggt atatttttgg
      841 agactggata ggtaaaatat attctcttag aaaaaatggg agtgtctggg aaaatgaaac
      901 cctggaacag aaaaatcctc ctgcggaatt caagcatatc aacagttttg ctcaggattc
      961 atccggtgat gtctatatac ttggacagaa ggatgtcggc gtgggaaaca aaggttatat
     1021 atataaaatc aggtttgccg aaaaatgaat cctgtatgaa atacggatca ttgtcaaacc
     1081 tgttctgttc agaaaactca tttgatgcag gcaaaaaaat cggacaacag agtcagtaat
     1141 gaattaaatc ttgacctgca gactttctta aaaaaataga gcttaggata cagtatgatc
     1201 agaatcgaat ttgaaaacga caagacagta gagacagaag caggaaaatc gattcttcag
     1261 atttcgctgg aaaatgaaat tccgcatttc catgcctgcg gaggtcatgc cagatgttcc
     1321 acatgcagga ttgcagtgct gaaaggactc gaaaatcttc ctggaagaaa tgaaaaagaa
     1381 aagaaactcg caaaggcaaa aggtttcgag gatgagatca gacttgcctg ccagactgtt
     1441 cccagttcgg atataaaaat cagaagactt gtaaatgata ctgaggatat gcagtctgca
     1501 gtcgcggaac acggaaacac aagcggaaga gaagcaaaac ttgctgtgct tttttcagat
     1561 atcagaggat atacttcttt ctcggaaaaa gctcttcctt acgatgcagt gcacatactg
     1621 aacagatact ttgcgagaat gggagactct gtcagagcaa acagaggctt tatagacaag
     1681 tacatcggcg acgggatgat ggtgctgttc ggtttgaacg gagagactcc atatatggca
     1741 gcatcagctg cagtcagaac tgccgtttct atggtcaaca gcctgaatga gctgaacata
     1801 tatctgaaga aaaactttga cacaaagttt cagattggaa taggaattca ttatggaaat
     1861 ctcattgtag gagagatcgg gcattcatcc aaaagacagt ttactgccat cggggactcc
     1921 gtaaacttcg caagcagaat cgagtcatac acaaaacagg ctg
//
LOCUS       DAOQGJ010000732         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40124_length_1961_cov_2.981112, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000732 DAOQGJ010000000
VERSION     DAOQGJ010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40124_length_1961_cov_2.981112"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK683_11580"
     CDS             complement(<1..195)
                     /locus_tag="PK683_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09134.1"
                     /translation="MRILLSFLLMLSCRTNSPIKNAVQSKYAGIYAMRDAVYMTKFFK
                     MNYKYRDEGLHWYCMDLDTER"
     gene            308..562
                     /locus_tag="PK683_11585"
     CDS             308..562
                     /locus_tag="PK683_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNH09135.1"
                     /translation="MITEGFAVSKSRKENQMKVKYSLSGMTCGHCAMTVQKIFAKEGI
                     QASADFKNGTVELESEANETVLSAVKMNLDEEGYSLGSKV"
     gene            565..>1961
                     /locus_tag="PK683_11590"
     CDS             565..>1961
                     /locus_tag="PK683_11590"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNH09136.1"
                     /translation="MEEAKPKEELTLDLFGMTCANCALRIERGLSRLSGMEEARVNLA
                     RETAFIRFSPGLSQEEILAKVEDLGYKASVHNEKSIKSAEDEHRKDFRKLKFRFFLSA
                     LFSVPLLYSMVSHFPALSFLPDPGILMHPWIQFLLAAPVQFWVGFSYYKGAYHALKNR
                     SSNMDVLVALGTSAAFGYSLISSFSAGSANGDIFYIQNGLHPHHSAFPPLYYETSAVL
                     ITFLIAGKWMETAAKGKSSDAVKTLLSLKAENARIRKGEEWTLVPSEYLKPGDIFLVK
                     PGEKIPADGTVLEGFSAVDESMLTGESIPSEKTVQSRVMGGTVNGSGVLTVRADRTGN
                     ETLLASIIKTVEDALSSKAPIQKIADRISAYFVPVVVLISVLDFILWFVFIQPGNFAG
                     ALEKAIAVLVIACPCALGLATPISLLVGTGKAASYGILFKNAETLESAATLQTIAFDK
                     TGTLTRNEMTVRRVVA"
BASE COUNT          526 a          421 c          457 g          557 t
ORIGIN      
        1 cctctctgta tccaaatcca tacagtacca atgaagccct tcatctctgt atttataatt
       61 cattttaaaa aactttgtca tatacacagc gtctctcatt gcatagattc ctgcatattt
      121 ggattgaact gcgtttttga ttggtgagtt cgtcctgcag gacagcatca gcagaaatga
      181 cagcagaatt ctcatcttca ctccttggga tcagttcttt tattgatcct gtcagcagtt
      241 tcggtcaatg caaaatccaa attttttata atttcctctt gaccgcactg tcgacgggaa
      301 aaggaaaatg ataacagagg gatttgccgt cagtaaatcc cgcaaggaga atcagatgaa
      361 ggtaaaatac agtctttcag gaatgacctg cggacactgt gcaatgacag tgcagaagat
      421 ttttgcaaaa gaagggattc aggcatctgc agattttaaa aatggaacag tggaactgga
      481 atcggaagca aatgaaaccg ttctttcagc agtaaagatg aatctggatg aagaaggata
      541 ttctttaggc tctaaggtct gattatggaa gaagcgaaac cgaaagaaga actgactctt
      601 gatctgttcg gaatgacctg tgccaactgt gctctcagaa tagagagagg tctctccagg
      661 ctttccggta tggaagaagc cagggtcaat cttgccaggg agactgcatt catccgtttc
      721 agtcccggac tttcgcagga agagattctt gccaaagtgg aggatctggg atataaggca
      781 tctgttcata atgaaaaaag catcaagtct gcagaagatg aacacagaaa ggattttcgg
      841 aagctgaaat tccgcttctt tctttctgct cttttttcag ttccgcttct gtattcaatg
      901 gtttcgcatt ttcctgcact ttcctttctg cctgatccgg gaattctgat gcatccctgg
      961 attcagtttc ttctggcggc tcctgttcag ttctgggtag gtttttccta ttataaagga
     1021 gcttatcatg ctctgaagaa cagatcttcc aatatggatg tactcgtggc actcggaaca
     1081 tcggcggcat tcggttacag cctcatttcg agtttttctg ccggatctgc aaatggagat
     1141 attttttata tacaaaatgg tctccatccg catcattctg catttccgcc tctctattat
     1201 gaaacatcgg ccgttctgat taccttcctg attgcaggaa aatggatgga aacagcggcc
     1261 aaaggaaaga gttcagatgc agtgaaaacc cttctgagtc tgaaggcaga aaatgccaga
     1321 atcagaaaag gcgaggaatg gactcttgtt ccctcagagt atctgaaacc tggtgatata
     1381 tttctcgtca agcctggaga aaagattccc gcagacggaa ctgttctgga aggattcagc
     1441 gcagtggatg aatcaatgct gaccggcgag agcattcctt ctgaaaagac cgttcagagc
     1501 agagtgatgg gaggaaccgt aaacggaagc ggcgtgctga ctgtaagagc agacagaaca
     1561 ggcaatgaaa cccttctggc atccatcatt aagactgtgg aagatgcact ttcctcgaag
     1621 gctcccattc agaaaattgc tgatcgtata tctgcttact ttgtgcctgt cgttgtgctg
     1681 atctccgttc tggattttat tctctggttt gtatttattc agccgggaaa ttttgcgggt
     1741 gctctggaaa aggcgattgc agttcttgtc attgcctgtc catgtgctct cggacttgcc
     1801 acccctattt ctcttctggt cggaacaggg aaagccgcat cctatggaat tcttttcaaa
     1861 aatgcagaga cgctggaatc tgctgcgact cttcagacga ttgcatttga caagaccggt
     1921 acactcacgc gcaacgaaat gacggtgcgg cgagtcgttg c
//
LOCUS       DAOQGJ010000733         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40178_length_1959_cov_3.929622, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000733 DAOQGJ010000000
VERSION     DAOQGJ010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40178_length_1959_cov_3.929622"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK683_11595"
     CDS             complement(<1..353)
                     /locus_tag="PK683_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09137.1"
                     /translation="MKNFISKLMNLGNEHFTKETDIKNNIIANQIILIFISGIFFFIL
                     YLICAMFYMIHLKQDIIVPAYHFVPPTFSMVAVLVLSFFLKYKTGKNTLIISIAYILG
                     IAYLTYMDYIVGPTVL"
     gene            complement(424..705)
                     /locus_tag="PK683_11600"
     CDS             complement(424..705)
                     /locus_tag="PK683_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09138.1"
                     /translation="MFSKYRENNLNFSSSDLSPAEFGDLHDTVQMEVMSKGDLQFRIF
                     KVWLGENPVRNGFRKKRPVYIRLNKSVLLRIQKDRDSPISSNEIEFPED"
     gene            1001..1789
                     /locus_tag="PK683_11605"
     CDS             1001..1789
                     /locus_tag="PK683_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09139.1"
                     /translation="MTDFLFSSTQSFFLIATAVFFLHCSSAKEKEHPIEEPDILYSNY
                     QNSKGMIIHKNIKVQMKTADALADIENFKENDYKKYENVLTERLQTVDDKLGHLKIDG
                     WKYISAKSILLIALTSALIIPIPGTFETITAAGFIVMGVQRFRETEEDKVNRIKLSDL
                     LDECAEAHDNLKELKKNSLPDFYKKWHKNFNDKNHHVKNISDPTDIRIYDRFWNTYTA
                     EKEHIIKSIEIELEHADNQCSYIKLKPEINANQPKIENWSDSPK"
BASE COUNT          692 a          361 c          360 g          546 t
ORIGIN      
        1 agaactgtag gaccgactat ataatccata tacgtcagat aggctattcc caatatataa
       61 gcaatactga tgattaatgt attttttcct gtcttgtatt tcagaaagaa actgagaacg
      121 agtaccgcaa ccattgaaaa agtagggggg acaaaatgat aggcgggaac aatgatatcc
      181 tgtttcagat ggatcatata aaacatcgca cagatcagat acagaatgaa aaagaaaatt
      241 ccggaaatga atatcagaat tatctgattt gcaataatat tattctttat atccgtttct
      301 ttggtaaaat gctcatttcc caaattcatc agtttggaaa taaaattctt cataaaacag
      361 cctctgacat atgcagtatt tctatcaaat tcataagatg cagaaagtca acttatataa
      421 agctcaatct tcaggaaatt caatttcatt ggaggatatc ggactgtctc tgtccttctg
      481 gattctcagc agtacggatt tgttcaaacg tatgtagaca ggcctttttt tacgaaatcc
      541 gtttctgacg gggttctctc ccagccagac tttgaaaatt ctgaactgaa ggtcaccttt
      601 gctcataact tccatctgca cggtatcatg caaatctcca aattccgcag gactcagatc
      661 ggatgaactg aaattcagat tgttttctct gtattttgaa aacaatacaa gtaggctcag
      721 cttctgaaag ttctcttcag caggaaaaac cggaagaatc ggaaaaacaa agcagattat
      781 caataaagat tttaaaacag tcatcacggt cattaaggga tttggaaaaa accaaagctg
      841 gtcaatatgc agcttctaat cggcctctac tttggcaaaa tgatttttaa caatctgaat
      901 ttttatttaa gaaagctttc tcatcagcac tgattatgtt tttcatctca gagcaaatca
      961 ttttattgac aggtaagtgc agctgcagga aattataacc ttgaccgatt ttcttttcag
     1021 ttcaacacag tccttctttt tgatcgcgac ggctgttttc tttcttcatt gcagttccgc
     1081 caaggagaaa gaacatccga ttgaagaacc cgacatactt tactcaaatt atcagaattc
     1141 gaagggtatg attattcata agaacatcaa agttcagatg aagacagcag atgcattggc
     1201 tgacattgaa aattttaagg aaaatgacta taaaaaatat gaaaatgttc tgactgaaag
     1261 acttcagact gttgatgaca aacttggaca tctgaaaatt gacggatgga aatacatcag
     1321 cgcaaaaagc atacttctga ttgcactcac atctgcactg atcataccga ttccgggaac
     1381 tttcgaaaca attactgcag caggattcat cgtgatggga gttcaacgat tcagagaaac
     1441 tgaagaagac aaggtgaatc gcataaaact gtccgatctg ctggatgaat gtgctgaggc
     1501 tcatgacaat ctgaaagagc tgaaaaagaa ttctctgcct gatttctata aaaaatggca
     1561 caaaaacttt aatgataaga atcatcatgt aaaaaatata tctgatccga cagacataag
     1621 aatctatgac cgattctgga acacttatac tgccgaaaaa gaacatataa taaaaagcat
     1681 tgaaatagaa ctagagcatg cagataatca gtgttcatat ataaaactga aaccggaaat
     1741 caatgcgaat cagccaaaga ttgaaaactg gtcggacagc cctaaataga ctgcagaaag
     1801 cagcggagaa gcttggcaaa gtgccgggaa acagaaatga caacgagtgc acttcactta
     1861 agtaaaatca tttgaaagaa ataacagagt atccgggctg acccgatcat aggcaaaatg
     1921 aaatattttt tcaaaactac tcttctgggt cttctgctt
//
LOCUS       DAOQGJ010000734         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40233_length_1958_cov_2.277982, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000734 DAOQGJ010000000
VERSION     DAOQGJ010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40233_length_1958_cov_2.277982"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK683_11610"
     CDS             complement(<1..366)
                     /locus_tag="PK683_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09140.1"
                     /translation="MINKISFKNYKLFRTLQEMELRPMTVLIGKNSSGKSAIAKLFTL
                     IEGSLSGSFNEAFRLINGGVELGAEFKDLIHERKYGQLDFVISSDSESLETKIITFER
                     QSPEIAEWKYHKGTAITELD"
     gene            602..1594
                     /locus_tag="PK683_11615"
     CDS             602..1594
                     /locus_tag="PK683_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNH09141.1"
                     /translation="MANLKHPKDALFGGEKPFPIIPACEHFAGSEKLITKAMELQNKM
                     GGLFDITMDCEDGAQTGKEKEHAEMIVRIQNSDLNVHKMSGVRIHDYTNAHWKDDVDV
                     IIPGAGNKIAYITIPKPTKASQVEEMITYIQSACAKAGIKREIPIHVLVETHGALAEV
                     EKIAKLPWLQVLDFGLMDFISGHHGAIPASCMRSPGQFEHEILRRAKSTMVAVALANG
                     VIPAHNVTLDLKNQYQTYADASRARNGFGFLRMWSIYPTQIQAILDAMAPDYSEVLDA
                     SEILIKAQDADWGPIQHAGELHDRATYRYFWELVQKAKITGQKLSDEVMKRFFS"
     gene            1604..>1958
                     /locus_tag="PK683_11620"
     CDS             1604..>1958
                     /locus_tag="PK683_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNH09142.1"
                     /translation="MTQVPEIVPVGTLPPVGVVPKKMHAQVIRQNRFGEPTNAFQVEQ
                     IDIPELAPDEVLVGVMAAGVNYNNVWAALGYPVDVIGARMKKGEKEDFHIGGSDASGI
                     VYKVGSNVKNVKVGDE"
BASE COUNT          578 a          438 c          440 g          502 t
ORIGIN      
        1 atccaattcc gtaatagccg ttcctttgtg atatttccat tctgcaattt ctggagactg
       61 tctttcgaaa gtgattattt ttgtttccaa actctcactg tctgacgaaa ttacaaaatc
      121 taattgaccg tattttctct catgtataag gtctttgaac tcagccccta attccactcc
      181 tccattaatg agacgaaatg cttcattgaa tgatccggaa agagagcctt ctatcaaagt
      241 aaataatttc gcgattgcac tttttccaga actgttcttg cctatcaaaa cagtcattgg
      301 ccgcaattcc atttcctgaa gtgttcggaa aagtttatag tttttaaaag atattttgtt
      361 tatcataaga gatattcgaa ttccatcggg atccaaaccc caagtcttca ttcgtattct
      421 gaaaggctgt tccaaggatg tcaaggaaag aactcgaata cttgacaaaa tcttccttcc
      481 cgccatcccc gtcgatcacc gcttctgaaa atccgaagtt tttgggttga gtacagggat
      541 ttacaaaaaa atctgtgatt aaaaccgggg taatccggtg aaaactgtat aaggaacgat
      601 tatggcaaac ttgaagcacc ctaaagacgc actgttcgga ggggagaaac catttcctat
      661 tattccagcc tgtgagcatt tcgccggctc cgaaaaactg attacaaaag caatggaact
      721 tcagaataaa atgggcggtc tctttgacat tactatggac tgtgaagacg gagcgcagac
      781 tggtaaagaa aaagaacatg ctgaaatgat cgtccgtatc cagaactctg atttgaacgt
      841 tcataaaatg agcggcgtga gaatccacga ctatacaaat gcacattgga aagatgacgt
      901 agatgtaatt atcccgggcg caggcaataa aatcgcttat attactattc ctaagcctac
      961 aaaagcatct caggtggaag agatgatcac ttatatccag agtgcttgtg caaaagcggg
     1021 aatcaaaaga gaaatcccta ttcacgttct ggtagaaact cacggagctc tcgctgaggt
     1081 ggaaaaaatc gcaaaactcc cctggctcca ggttctcgac ttcggtctga tggacttcat
     1141 ctcaggacat cacggtgcaa tccctgcttc ctgtatgaga agccctggac agtttgagca
     1201 tgaaatcctg agaagagcaa aatccactat ggtggctgta gctctcgcaa acggggtaat
     1261 tcctgctcat aacgtgactc ttgacctgaa aaaccagtac cagacttacg ctgatgcttc
     1321 ccgtgcaaga aacggattcg ggttcctgag aatgtggtct atttatccta ctcagatcca
     1381 agcaatcctc gatgcaatgg ctcctgacta ttcagaagtt ctggatgcat ctgagatcct
     1441 catcaaagct caggacgcag actggggtcc aatccagcac gctggagaac tgcatgacag
     1501 agcaacgtac cgttatttct gggaactcgt tcagaaggcg aaaatcaccg gtcagaaact
     1561 gtccgatgaa gtaatgaaga gattcttcag ctaaggggaa aatatgactc aagttccaga
     1621 aatcgtcccc gtaggaactc ttccgccggt cggagtggtt cctaagaaga tgcacgctca
     1681 ggtgatccgc cagaacagat tcggcgagcc tacaaatgca ttccaagtcg agcagatcga
     1741 cattcctgaa ctcgctccgg atgaggtgct tgtaggagtg atggcagcag gcgtgaacta
     1801 caacaacgtc tgggctgctc tcgggtatcc ggtcgatgtg atcggtgcga gaatgaaaaa
     1861 gggtgaaaaa gaagatttcc acatcggcgg ttcagatgcc tccgggatcg tctacaaagt
     1921 cggctccaat gtaaaaaatg taaaggtcgg tgacgagg
//
LOCUS       DAOQGJ010000735         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40248_length_1957_cov_4.143533, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000735 DAOQGJ010000000
VERSION     DAOQGJ010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40248_length_1957_cov_4.143533"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1957)
                     /locus_tag="PK683_11625"
     CDS             complement(<1..>1957)
                     /locus_tag="PK683_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNH09143.1"
                     /translation="NDRLWVKENIFDERNDYGWSSKERTTPSEEFLLLDLGSINRVDE
                     FRILSKDSDETFFPEQFLFYYSEDDLTWQLLHEEPNFIAEPGTWYKWKFFPTNMRYFK
                     LVMANNKINSAKKYACQIIELEIFAAPDYLSLSKKKIINETPPYSSAIRSGLVRLAQD
                     GETNPSVAVQGNDRRLRDATTEFKGIVELATDGEERPLVAVQGNDSRLKKATENTFGL
                     VRLAKNGENRSETVVQGNDDRLKPATVSNMGIVELAEDGETRPNTAVQGNDSRLKKAT
                     VKDYGLVMLAELGEDTPGKVVTGDDPRFKNATTEKEGILRFASNGESAGFAAVQGNDK
                     RLKKATTESLGIVELAHDGETKDNTVVQGSDSRLKPASVTNAGIMQFADYSVKVPLKA
                     VQADDPRLYDDRDPKPHSHDYADKIHTYDSHTGLIHITGETGSGIKNIVPPVQNQSVI
                     FGKNSGKNGSGISGIGTDEGVSGYGEDFGVLGMSNSIHDDSAGIAGFGKKGYGGIFIS
                     QKNYAVYANGEGMKRRDMHSSGKAVLAKGDSDFFGKVRVINEKGDDCIARFFKCISGD
                     IIRKGDIVSIGEREQTVVISKSSYSTRVLGVCTESSSLEFGEQKTGNDFYLIAVYGIV
                     RINVDASEGGAIQPGDLLVSSLTGGYAC"
BASE COUNT          511 a          480 c          354 g          612 t
ORIGIN      
        1 tgcaagcata acctcctgtc aaagaagaaa caagaaggtc tccgggttga attgctccgc
       61 cctctgatgc atcgacattt attctcacta tcccataaac agcaataaga taaaaatcgt
      121 tccctgtctt ctgttctcca aattctagag aggacgattc cgtgcagaca cctagaactc
      181 ttgtagagta tgaagatttt gagattacta cagtctgttc cctttcaccg atggagacaa
      241 tatcaccttt tctgataatg tctcctgata tgcacttgaa aaatcttgca atacagtcgt
      301 cacctttttc attaatgact ctgaccttgc cgaaaaaatc ggaatctcct ttggcaagca
      361 ctgcttttcc ggaagaatgc atatcccttc ttttcattcc ttccccgtta gcatataccg
      421 cataattttt ctgggatata aaaattccac catatccctt tttgccaaaa cctgctattc
      481 cagctgaatc atcatgaata ctgtttgaca tcccaagaac accaaagtct tcaccatatc
      541 cgctgactcc ctcatcagtt cctataccgg aaatccctga accgtttttg ccgctgtttt
      601 tgccgaagat gacagactga ttttgaacag gaggcactat attctttatt cccgaccctg
      661 tctctccggt aatatgaatt aagccggtat gcgagtcata agtatgaatt ttatcagcat
      721 aatcatggct gtgcggttta ggatccctgt cgtcataaag cctcggatcg tctgcctgaa
      781 cggccttaag agggactttc acactgtaat cagcaaactg cataatgcct gcatttgtga
      841 cagaggctgg tttcagtctg gaatcacttc cctgaaccac tgtattatct tttgtttctc
      901 catcgtgtgc aagttcaaca attccaaggg attcagtggt agcctttttc agtctcttgt
      961 catttccctg aacagccgca aatccggcac tctcaccgtt cgatgcaaat ctcagaattc
     1021 cttctttttc agttgttgca ttcttgaatc ttggatcatc tccggttaca actttccctg
     1081 gagtgtcttc accgagttca gcaagcatta ccagaccata atctttgaca gtcgcctttt
     1141 tcagtcttga atcatttccc tgaacagccg tattcggtct agtttccccg tcttcagcaa
     1201 gttcaacaat tcccatattg gaaacagtcg ccggtttcaa acggtcatca tttccctgaa
     1261 caactgtctc tgatctgttt tctccgtttt tggccagtcg gacaagccca aaagtgttct
     1321 cagttgcttt tttcagacga gaatcatttc cttgaactgc aacaagaggt ctttcttcac
     1381 cgtcagtggc aagttcaaca attcctttaa attcggtagt cgcatctctc agacgacggt
     1441 cattcccctg tactgccacg cttggatttg tttcgccgtc ctgcgccagt cttacaagac
     1501 cggaacggat tgcggaagag tagggcgggg tctcattgat aattttcttt ttggaaagag
     1561 aaagatagtc cggagcggca aaaatctcaa gctcaatgat ctgacatgca tatttcttcg
     1621 cactgttgat tttattattt gccattacca gtttgaaata tctcatattg gtagggaaaa
     1681 atttccattt gtaccaggta cccggctcgg caataaaatt tggttcttca tgaagaagct
     1741 gccatgtcag atcgtcttca ctgtaataaa ataaaaactg ttcaggaaaa aaagtctcat
     1801 cagagtcttt cgaaagaatt ctgaattcat ccactctgtt aattgaacca agatccagaa
     1861 gaagaaattc ttccgaagga gttgttctct ccttggaaga ccagccgtaa tcgttcctct
     1921 catcaaaaat attttctttt acccagagcc tgtcatt
//
LOCUS       DAOQGJ010000736         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40341_length_1955_cov_2.457368, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000736 DAOQGJ010000000
VERSION     DAOQGJ010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40341_length_1955_cov_2.457368"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PK683_11630"
     CDS             <1..439
                     /locus_tag="PK683_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09144.1"
                     /translation="PGWGQWEDGRTGKGIGYGSAFFLLSLNYYIKYANFNAKEKTYRS
                     TLFLPATQEQDTFLLNYVLLSRPKSDYKSAEGEVNTAFYLLSGFYLWNLFDAYYFSAG
                     NIQGAAPSAFRFNFLPGKRLISSAASGSAFRENYWEASFEYKF"
     gene            466..>1955
                     /locus_tag="PK683_11635"
     CDS             466..>1955
                     /locus_tag="PK683_11635"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH09145.1"
                     /translation="MKRIIFYALLLFFPFLTDAQSRDRKNVRLPNSGRKGLMIASEED
                     IKSFEGTKRYALIVGVNGYKNLNKLSFAVGDSLKMKLTLEQIGKFNKVVLLNDESGEK
                     DSSLLPTRVNIEREFKSMIKDHPKLLLFYFSGHGFMNSAGENVIAPMDVIYESKKNVS
                     NYLSIHQLVKSASVLDQAVFLIDACREKLTDNTKSVEADKYGDFPENVRNAKGVAIMM
                     GTKPGGFSYENPKLGSGTFTHFLIEGMNGGVQDDGLEYVTFGKLKEYVESHMGEYTKK
                     ETNKKQTPYTAGDYTGNFLLAVGRPKGEVKSQTVKYQDKSKNVRLARVLKDTSGKKIS
                     QNFFVISGSDTYVPADLEGVAKMKFEYDSSGYNARQYSLKGELLFEYGFNYKDEYSSV
                     LKMLDENSLPVIKTFHRESDKITQTLSDKTGNPAEDSSGIHKTVYMHDGKNIISETYY
                     DLSGKPADNKFKIHKKIYQYDSAGRKISEEFRNLNDQLTEDRDRYGT"
BASE COUNT          605 a          365 c          448 g          537 t
ORIGIN      
        1 tcccggctgg gggcaatggg aggacggaag gacagggaaa ggaatcggct atggttctgc
       61 tttttttctg ctgagtctga attattatat aaaatatgca aattttaatg caaaggagaa
      121 gacttaccgt tcgactctct ttcttcctgc gactcaggag caggatacat tccttctgaa
      181 ctatgttctt ctctccagac cgaagagtga ttacaagtca gctgagggag aagtgaatac
      241 cgcgttttat cttctttccg ggttttatct ttggaatctt tttgatgcct attatttttc
      301 tgcaggcaat attcaaggag ctgcaccatc tgccttcaga tttaatttcc tgcccggaaa
      361 aagacttatt tcctctgccg catcaggttc tgcattccgg gaaaattact gggaagcatc
      421 ttttgagtat aaattttaat gaaaaaacat gacaggagtc catatatgaa aagaattatt
      481 ttttatgctt tgctgttatt ttttccattt ctgacagatg cccagtccag agacaggaag
      541 aatgtacgtc tccctaattc tggaagaaag gggctgatga ttgcttccga agaggatata
      601 aagtcatttg aaggcacgaa aagatatgct ttgattgtcg gtgtaaacgg ttataagaat
      661 ctgaacaagc tgagttttgc ggtcggggat tctttgaaga tgaaactgac acttgaacag
      721 attggaaaat tcaacaaggt tgttctgctg aacgatgaat ccggagaaaa agattcctct
      781 cttcttccga cacgtgtcaa tattgagagg gagttcaagt cgatgataaa ggatcatcct
      841 aagcttcttc ttttttactt ttcaggccac ggatttatga actctgccgg agagaatgta
      901 attgcaccta tggatgtcat atatgaatcc aagaagaacg tatccaatta tctgagtatt
      961 catcagctgg tcaagtctgc ttctgttctg gatcaggcgg tttttctgat tgatgcctgt
     1021 cgtgagaaac tgacggacaa tacgaaaagt gtagaggccg acaaatacgg tgacttcccg
     1081 gagaatgtga gaaatgcaaa gggtgttgcc attatgatgg gaaccaagcc cggcggattc
     1141 tcttatgaaa atcccaagct tgggagcggc accttcactc attttctgat tgagggaatg
     1201 aacgggggcg ttcaggatga tggcctcgaa tatgtgacat tcggaaaact gaaagagtat
     1261 gtcgagtctc atatgggaga atatacgaag aaggaaacga acaaaaaaca gactccatat
     1321 acagcaggcg actatacagg aaattttctt ctggcagtcg gccgtcccaa aggagaagta
     1381 aaatcacaga cagtaaagta tcaggataaa tcaaaaaacg tccgcctcgc cagagtgctg
     1441 aaggatactt caggaaaaaa aatcagtcag aatttttttg tcatttccgg ttcggatacc
     1501 tatgtccctg cagacctgga aggtgtggca aagatgaagt ttgaatacga ttcatccggt
     1561 tataatgcaa gacagtattc actgaaagga gaacttcttt ttgaatacgg attcaattat
     1621 aaggatgaat acagttctgt gctgaaaatg ctcgacgaaa actctcttcc cgtcattaag
     1681 acatttcaca gagaatcgga taaaatcact cagaccttgt ccgacaagac gggcaatccg
     1741 gctgaagaca gttccggaat tcataagacc gtgtatatgc atgacggaaa aaacataatt
     1801 tcggaaacat actatgattt gtccggaaaa ccagcagaca ataaattcaa gattcataag
     1861 aagatatacc aatatgattc tgccggccgg aaaatttcag aagaattcag aaatcttaat
     1921 gatcagctga cagaagacag ggacaggtat gggac
//
LOCUS       DAOQGJ010000737         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40465_length_1951_cov_3.422468, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000737 DAOQGJ010000000
VERSION     DAOQGJ010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40465_length_1951_cov_3.422468"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..370
                     /locus_tag="PK683_11640"
     CDS             155..370
                     /locus_tag="PK683_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09146.1"
                     /translation="MKKILTGLVLLSLAYCNDFDITGNKQREKEQKEKENNSNLIFIA
                     CYSEYTAGRMSYTAYTNCISASRISGQ"
     gene            389..>1951
                     /locus_tag="PK683_11645"
     CDS             389..>1951
                     /locus_tag="PK683_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HNH09147.1"
                     /translation="MNGKFLHVFLFLVFVFSARFGIGIVSNTALPQALPGIQVSGGNL
                     NATVDISVPEGTHGLTPNLDLVYNSAAGTGLLGLGWELSGLHSVSRDPSYPISYSDSD
                     RFSSSVSGGLIQNGGTQEYGTNPETLMKYKFNGESWTAKDRSGTVYQFGSDDSRNSRV
                     KSPERGTDRNAVRKWGLDSVKDLNGNSYTVDYDGSQELLPVKIEYGSGNRTVEFGYDS
                     ALKNKGAREGYVYSSKVLEESILKSITMKAGGTKVREYVFSYDVSSADGIPRLNKIDR
                     TDYINGQAEFYSIALGYTADQAVSISQTPKSLDLDETFVLSRTPMSDKMTCVQDERDC
                     YEAANTNCVSGATYISCRTRHNEVIRACNEYTRLWKNPCFLGVTLPNNTMTVGDVNAD
                     GKPEIFRLIGSEDAKNIRIAKTSVNADGSLSDDKNSSPVLQNVNYRTFSTMGWGDADG
                     DGKTDFAYSDGANLLVMYSNGSGFDPPVRMANVSAVIPAFNYFTDMPDRKWKGGLIDI
                     NGDGKLDVVVVGE"
BASE COUNT          578 a          390 c          504 g          479 t
ORIGIN      
        1 gagtccagcg ggaattgtgt aatgataaag tatattagga agatgaaaac tattttcatg
       61 gatcttactg ctattcccca ctggctgaac tcttttgctt cgccgcttcg ctatctcatt
      121 ccaaactgga ttcattgtac aaaaggagaa aactatgaaa aaaattctga caggactggt
      181 actgctgtct ttggcatact gtaatgattt tgacattaca gggaataagc agagggaaaa
      241 agaacagaag gagaaagaga acaattcgaa tctgatcttc attgcctgtt attctgaata
      301 cactgcaggc agaatgtcgt atactgcata tacgaactgc atcagtgcaa gcaggatcag
      361 cggtcagtag gaaattcagg gaggaaagat gaacggaaaa tttctgcatg tatttttatt
      421 tttggtattt gtattctctg ccaggttcgg tatagggatt gtatcaaata ctgcccttcc
      481 gcaggctctg cccgggatac aggtcagcgg cggaaatctg aatgcgacag tggatatttc
      541 cgttcctgaa ggaactcacg gtctgactcc caacctggat cttgtctaca attctgcggc
      601 aggtactgga ctgcttggac ttgggtggga actttccggt ctgcattctg tttcgagaga
      661 tccctcctat ccaatcagtt attctgattc agaccgtttc agcagttctg tttccggagg
      721 actgatacag aacggaggaa cgcaggaata cggaacaaat ccggaaaccc tgatgaaata
      781 taagtttaac ggggagagct ggactgcgaa ggaccgatcc ggcactgtgt atcagttcgg
      841 atcagacgat tcgaggaatt ccagagtaaa gtctcctgaa agaggtacgg acagaaatgc
      901 agtccgtaaa tggggactgg attctgtgaa ggatctgaac ggaaattcct ataccgtaga
      961 ttatgatgga agtcaggaat tgcttcctgt aaaaatagag tacggttcag gaaacagaac
     1021 ggttgaattc ggatatgact ccgctctgaa aaacaaagga gccagagagg gatacgttta
     1081 ttcatccaag gttctcgagg aaagtattct gaagtccatt acaatgaaag caggcgggac
     1141 aaaagtcagg gagtatgtat tttcctatga tgtcagttca gcagacggaa ttcccaggct
     1201 gaataaaata gacaggactg actatataaa cggacaggcg gaattttatt ccatcgcatt
     1261 ggggtacact gctgaccagg cagtcagtat atctcagaca ccgaaatcat tggatctgga
     1321 tgaaacattt gtgctgagca gaactccgat gagcgacaaa atgacctgcg tgcaggatga
     1381 acgggactgc tatgaggcgg caaatacaaa ctgtgtctcc ggtgcaacat acatatcctg
     1441 cagaacaagg cacaatgaag tgatccgggc ctgtaatgaa tatacaaggc tctggaaaaa
     1501 cccctgtttt ctgggagtga ctcttccaaa caatacaatg acagtcgggg atgtaaatgc
     1561 ggacgggaaa cctgaaattt tccggctgat cggctcagaa gatgcgaaaa acatccggat
     1621 tgcaaagacc tcagtgaatg cagacgggag tctttcagac gacaagaaca gctctccggt
     1681 tctgcagaat gtcaattaca gaaccttcag tacaatggga tggggggatg cagacgggga
     1741 cgggaaaact gattttgcct attcggacgg agcgaatctg cttgtcatgt actcgaacgg
     1801 aagcggtttt gacccacctg tcagaatggc caacgtgagt gcggtcatcc ctgcattcaa
     1861 ttactttaca gatatgcctg acaggaaatg gaagggaggt ctcattgaca tcaatggtga
     1921 cggcaaactg gatgtcgtgg tcgtcggcga a
//
LOCUS       DAOQGJ010000738         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40641_length_1947_cov_3.151691, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000738 DAOQGJ010000000
VERSION     DAOQGJ010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40641_length_1947_cov_3.151691"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PK683_11650"
     CDS             <1..556
                     /locus_tag="PK683_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNH09148.1"
                     /translation="EIETIERVEIIRDLRKGIYEAVVGINLLREGLDIPEVSLVAILD
                     ADKEGFLRNYKSLIQTIGRAARNSNGKAILYAEKITPSMKQAIDETARRRKIQEEHNR
                     KFGITPLTIKKEIKDIISRQNSQEPETAEEIMLNEIEEQFTLKKYKTNEKLKEKLKEE
                     MLKAAKDLDFERAALLRDKMMSLK"
     gene            complement(766..>1947)
                     /locus_tag="PK683_11655"
     CDS             complement(766..>1947)
                     /locus_tag="PK683_11655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNH09149.1"
                     /translation="VHIAFIQAGMMEETFKILLILAVSFIFCFNRLDRTWTKDAVLAG
                     GFTALGFSLIENYVYIHKDPRNTVEMFIGRTVFSSNIHFLINLVFALFLLKSNKASSR
                     SEKAVIIGYAYGLAVIQHGVVDFFLIPGSRFGNWLASALFIGIWVWAVRDLRVLVYDK
                     VPESIAVDISDPEPTGAGLSAAELEKKYRGTENRFGWFEELYSKAGNDVSLIPWEDGF
                     PNPNLVQWAEKTGFNFQEKTCLVVGCGLGRDAEFLSGLGARTDAFDISESAISAAKNK
                     FPDSKVSYFARDLFDLENLEYDFVFESYTVQTFFEEEDRQRAAEKIAGFVSEGGHLLL
                     IARGREESEKTDNPPFPLSKSEILYFENFGLRVQEFEDYNEYEPSGKITRRFRVLFVK
                     D"
BASE COUNT          624 a          427 c          408 g          488 t
ORIGIN      
        1 cgagatagag acgattgaaa gagtggaaat cataagggat ctgagaaaag gaatatatga
       61 ggcggtggtc ggaatcaatc tgctcagaga aggactggat attcctgaag tgtctcttgt
      121 ggcaattctg gatgccgaca aggaagggtt cttaagaaat tacaaatctc tgattcagac
      181 aatcgggaga gcggcaagga attccaacgg aaaagccata ctgtatgcag aaaaaataac
      241 tccttccatg aaacaggcaa ttgacgagac tgccagaaga agaaagatcc aggaagagca
      301 caacaggaaa tttggaatca ctccacttac aatcaagaag gaaatcaagg atatcatctc
      361 cagacagaac agccaggaac cggagactgc tgaagagatt atgctgaatg aaattgaaga
      421 gcagtttact ctcaaaaagt acaagacaaa tgaaaaactg aaagagaagc tgaaggaaga
      481 gatgctgaag gcagcaaaag atctggattt cgaaagagct gctctgctca gggacaaaat
      541 gatgtcttta aaataaggca agtaatgcaa atggtgctta aacagatatt taaatttcta
      601 tcatttttaa tgatttaaaa ttttcatatc gtgaccagaa tcagacggtt tctgagactt
      661 atggatgcaa tttgaccgtc tgagaatgga ataattttac cgagtttagc cagtcctgct
      721 ctttcaactg aatgaagtcc tgctgactct gcagccttct gcagatcagt cttttacgaa
      781 cagaacacgg aaccttcttg taattttccc ggaaggttca tattcattgt agtcttcaaa
      841 ttcctgaacc ctgagaccga aattctcgaa ataaagtatc tctgattttg acagagggaa
      901 aggagggttg tccgtttttt ccgactcctc tcttcctctg gcaatcagaa gcagatgtcc
      961 gccttcggat acaaaacctg caattttctc cgcagccctt tgtctgtctt cttcctcgaa
     1021 aaaagtctga accgtatagg attcgaaaac aaaatcgtat tcgagattct ccagatcaaa
     1081 aagatccctt gcaaaataag ataccttact gtcaggaaac ttattttttg cggcagagat
     1141 tgcagattct gaaatatcaa atgcatccgt ccttgcaccg agacctgaaa gaaactcagc
     1201 atccctgccg agtccgcatc cgactacaag gcatgttttt tcctgaaaat tgaatcctgt
     1261 cttttctgcc cactgaacaa gattcggatt cgggaaaccg tcttcccagg gaatcagtga
     1321 aacatcgttc cctgcctttg aatacagttc ctcaaaccag ccgaatcggt tttcagttcc
     1381 cctgtatttt ttttccagtt ccgcagcaga gagacctgct cctgtcggct ccggatcgga
     1441 tatatccaca gcaatacttt caggaacctt atcatataca agtacccgga gatccctgac
     1501 tgcccagacc cagatgccga tgaagagtgc agaagcaagc cagtttccga acctcgaacc
     1561 cggaatcaga aaaaaatcca caactccgtg ctgaatgaca gcaagtccgt acgcatatcc
     1621 tataatgacg gctttttcgg atctggaaga ggccttgttg gatttgagca gaaacagcgc
     1681 aaaaaccaaa ttgatgagaa aatgaatatt ggaggagaat accgttcttc caataaacat
     1741 ctctacagta ttcctggggt ccttatgtat atacacatag ttttcaatca gtgaaaaacc
     1801 gagagccgta aatccgcctg caagcacagc atccttcgtc cacgttctgt ccaggcggtt
     1861 gaaacagaaa ataaatgaaa cagcaagaat cagcagaatc ttgaatgtct cctccatcat
     1921 ccctgcctga atgaatgcaa tatggac
//
LOCUS       DAOQGJ010000739         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40644_length_1947_cov_2.925476, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000739 DAOQGJ010000000
VERSION     DAOQGJ010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40644_length_1947_cov_2.925476"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PK683_11660"
     CDS             complement(<1..196)
                     /locus_tag="PK683_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09150.1"
                     /translation="MEGFSSFQTAMVRMQEISGMVEQMEKESSDKFLGQKPEFLKELE
                     RNVKEIHKNAEIREKKEIPIP"
     gene            279..>1947
                     /locus_tag="PK683_11665"
     CDS             279..>1947
                     /locus_tag="PK683_11665"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNH09151.1"
                     /translation="MRFYLSRCVSALPVLWAYEAGYFRDNGIDAEIKILDSYNSIEAA
                     LQDGRADAGEFFFSAYLLKANTVGSHNLLYFPAMFLNLSELVFYTDKNLFKQSKDYTP
                     KMILPVPHEGSPCCLYAKTMIRKFFGDVNEAPVSVMEMPYHMYEQIYSSEHCFGLVAD
                     NLIFPFITSPPHIHKLKEVDKVPGSVLCFSDRFILQNREKVVQIIEAVKKSITLLSNS
                     NPINESFFLGIIQKYQIPYLQKADTKLLREKFPEFFCFEVPDFCIEDLIFLLNQEHPE
                     NKNIKNLTRSIIIMMDNILDQSVPVQRLIKKVPVLKVTENNEEKDQFSFFEKKYGKHV
                     RNFGYSFYKALFTDSSELMLFFDRKTGRIIDANTRFKEELGYSEEIERLTIYDVIKDC
                     DENHPLHSFMQKAGETNYISNLELLHKNRHDLSADLYISPLIFAEKVFYLCVFINNSE
                     NKEAMRLRHEFISNISHELRSPMTSIRGFFELISTDANLNFNDEHRNVLNIIEKNILK
                     MIALIDNLLQLDKKDRLLETKIIEKFDPASVIEEIIMINEPLAKGKGL"
BASE COUNT          620 a          396 c          371 g          560 t
ORIGIN      
        1 ttgggatggg aatttctttt ttttcacgga tttccgcatt tttgtggatt tcttttacat
       61 tccgctccag ttctttcaga aactcaggct tctgtcccag aaatttatcg gaactttcct
      121 tttccatctg ttccaccata ccggaaatct cctgcatacg aaccatcgct gtctgaaatg
      181 atgaaaatcc ttccaattcc acagtactat tttcggattt ctggacaata aattcagtta
      241 aaacttgcac agtcattgaa aaaaatttag atggaagtat gagattttat ctgagcagat
      301 gtgtttctgc gcttccggtg ctctgggcat atgaagccgg ttatttccgt gataatggaa
      361 ttgatgcaga gataaaaata ttggacagtt acaacagtat tgaagccgcc ttgcaggacg
      421 gaagagcaga tgcaggagag tttttcttca gtgcctatct tctgaaagca aatactgtag
      481 gttctcataa ccttctgtat ttccctgcaa tgtttctgaa tctgagtgag ctggtgtttt
      541 acacagacaa gaatctcttc aaacagtcaa aagactatac tccaaaaatg atccttccgg
      601 tccctcatga aggctctcca tgctgtctct atgcaaaaac aatgatacgg aaatttttcg
      661 gggacgtgaa tgaagcacca gtatccgtaa tggaaatgcc gtatcatatg tatgaacaga
      721 tttattcatc agagcactgt ttcggtcttg tagcagacaa tctgatcttc ccgttcatca
      781 cctcccctcc tcatattcac aaactgaaag aggtggacaa ggtgcccggc tccgtcctct
      841 gtttcagtga caggtttata ctgcagaaca gagaaaaagt tgtacagatc attgaagcag
      901 tcaagaagtc aatcacactg ctctccaaca gcaatccgat caatgaaagt ttttttctgg
      961 ggatcataca gaaataccag atcccctatc ttcagaaggc cgacacaaaa cttctcagag
     1021 aaaagttccc tgaattcttc tgtttcgaag tgcccgattt ctgcattgag gatctgatat
     1081 tccttctgaa tcaggagcat ccagaaaaca aaaacatcaa gaacctgact cgatccatca
     1141 taattatgat ggataatatc ctggatcagt ctgtacctgt tcagagactc attaaaaaag
     1201 ttcctgtact gaaagtgacg gagaacaatg aggaaaaaga tcagttcagc ttttttgaga
     1261 aaaaatacgg aaagcatgta agaaatttcg gatacagttt ttataaggcc ctttttacag
     1321 attcatctga acttatgctt ttctttgaca ggaaaaccgg aaggatcatt gatgcaaata
     1381 cacgcttcaa ggaagaactc ggctattcag aagaaataga aagacttaca atatatgatg
     1441 ttattaaaga ctgcgatgaa aatcatccgc tccactcgtt tatgcagaag gcaggagaaa
     1501 caaactatat ctcaaatctg gaactgcttc ataaaaacag gcatgacctg tctgcagacc
     1561 tttatatcag ccctctgata tttgcagaaa aggtctttta cctctgtgta ttcatcaata
     1621 actcagaaaa caaggaagca atgagacttc ggcatgaatt catttcgaac atcagtcatg
     1681 agttaagatc tccgatgaca agcatcaggg ggtttttcga actgatttca actgatgcaa
     1741 atcttaattt taatgatgaa cacagaaacg tactgaatat aattgagaag aacatcctga
     1801 agatgattgc actgatcgac aatctgcttc agctcgacaa aaaagacaga cttttggaaa
     1861 ctaagattat tgagaaattt gatcctgcat cagtcatcga agaaatcatt atgatcaatg
     1921 aacctcttgc aaaaggaaaa ggactgt
//
LOCUS       DAOQGJ010000740         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40647_length_1947_cov_2.849366, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000740 DAOQGJ010000000
VERSION     DAOQGJ010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40647_length_1947_cov_2.849366"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PK683_11670"
     CDS             <1..327
                     /locus_tag="PK683_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09152.1"
                     /translation="IEPLEFRSLVSERIVITDKKDIEEISSSIRKAEPYFFNHPTPVW
                     TAAVRITTVEKEEFGGTVISSAEESQGVNFSVKSGIYEGFMYQRLKNNDLGPVIEKMA
                     AKYRKK"
     gene            complement(663..1562)
                     /locus_tag="PK683_11675"
     CDS             complement(663..1562)
                     /locus_tag="PK683_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNH09153.1"
                     /translation="MLTFLGCSLLNPSKDNSDKNKQIALAALALSASKTVCTSSSSTG
                     STVTNSTATLNSATGCVTGVNTCMDSALPSWIRDNFKCSVAYLDGTNIVFKSQNLPNH
                     KSYYWGSSSALFDSATAGNPSTIASQKMVYKIPGNAAKGSGTDSTQAGQVSIGITVNG
                     LAIFNNAAAPPDVLKNEAVSFDSYSGHPESTGTYHHHAAVPMVCDGTTLVSNSASCNN
                     SKLIGIALDGYAIYGQKCDKGTSSTSDDTAPTDLDSLHGHTAVTTHFSTATYHYHYAY
                     DATATIYTLMGSYFYGKQGSVSN"
BASE COUNT          561 a          350 c          464 g          572 t
ORIGIN      
        1 attgagccac tggaattccg ttctcttgtt tcggaacgaa tcgtgattac tgataaaaaa
       61 gatattgaag agatttcatc atcaatcagg aaagccgagc cttatttctt caatcacccg
      121 actcctgtct ggactgctgc agtcaggatt acgacagttg aaaaagaaga attcggcggt
      181 actgtcattt catctgcaga ggaaagtcag ggagtaaatt tttctgtgaa aagcggaatt
      241 tatgaaggtt ttatgtatca gagattaaaa aataatgatc tcggtccggt cattgaaaaa
      301 atggcggcga aatacagaaa gaaatagaag cttccctcat tttcaaaatg ataaaaaatt
      361 atttacaata cagatctgaa ttttgataaa aaataaaaga gttaaaaaga acaagtatcc
      421 tgtaaactga aggcatcgtt ggatagacag gacagtcagg tgatatatga tggattggaa
      481 aaagctataa gtcctttctc tgtattttca gtgattttac tgctttctga ttggactggt
      541 ttcagactgt caagagatgc ttggcagccg cttcaaattt tggcgtcttt ttgcgaaggg
      601 gctgcttaga ctccttcgct gtaaatttct gcgatgactc agtcaggttt tgtgcaatct
      661 tctcaattgc tgactgatcc ctgtttaccg taaaaatacg atcccattaa agtataaatt
      721 gttgcagtag catcatatgc gtagtgataa tgatatgtcg cagtgctgaa gtgcgtcgta
      781 actgctgtat gcccatgcag tgaatccagg tcagtcggag cagtgtcatc agatgtgctt
      841 gaagttccct tgtcacattt ctgcccgtag attgcgtacc cgtcaagtgc aattcctatg
      901 agttttgaat tattgcagga ggcactgttg ctgacaagcg ttgttccgtc gcagaccatc
      961 ggtactgcag catgatgatg ataagttcct gtggattcag ggtgccctga gtaagagtca
     1021 aatgatacgg cttcattttt cagaacatca ggaggggcag ctgcattatt gaatattgca
     1081 agtccgttga ctgtgatccc gatggatacc tggcctgcct gagtgctgtc tgtgcctgag
     1141 ccctttgctg catttcctgg aattttatat accatcttct gacttgcgat cgtgctggga
     1201 tttcccgcag tggccgaatc aaaaagtgca gaggacgagc cccagtagta acttttatga
     1261 tttggaaggt tctgtgattt aaatacaata tttgttccgt ccaaataagc tacagaacac
     1321 ttgaaattgt ccctgatcca ggagggaagg gcactgtcca tacaggtgtt tactccagtg
     1381 acacatccgg ttgctgaatt caaggttgcc gtgctgtttg tcacagtgga acctgtgctg
     1441 cttgatgaag tacagactgt cttggatgca gaaagggcaa gcgctgcaag ggcgatctgc
     1501 ttgtttttgt cggaattgtc tttggaagga tttagaagac tgcatcctag gaatgtaagc
     1561 atacagactg ctgtttttat tttaaatgac attttcattc tcctgtaatt taatgtaaat
     1621 ataatgtatt taatatttga tttaaagcaa gagtattaac tgatattgtc ggtgttctgc
     1681 tgcgtaatgg tactgaatga aatatgttct aacggcacgt ttattgcatg aaatacgaag
     1741 ggataggaaa tattgaaaag aacttggttc tgcagattca accccgctgc gtaccactgg
     1801 acactcgaag caaaaggaaa tgagccgagg atttggaaga cagaaaagga aaatattcgg
     1861 aaggattcag gaaacagact gcactgacac ctgcgggaag atggaaaaaa gcttaagtgc
     1921 tgagatggga gaatcagagg ctgaagg
//
LOCUS       DAOQGJ010000741         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40690_length_1946_cov_2.531465, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000741 DAOQGJ010000000
VERSION     DAOQGJ010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40690_length_1946_cov_2.531465"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PK683_11680"
     CDS             <1..374
                     /locus_tag="PK683_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09154.1"
                     /translation="ELSVQLSQFPRTKVGGGFSYVIPSWLIDLAIPGNMEELIWHFSQ
                     VLVHANNKKGTRMYMSLGAEGNSYKLNASADTEFLNNFFVRFALKVWNFKVAPDDEAK
                     EDMKKLIAKIARTVKEDLSKF"
     gene            complement(626..814)
                     /locus_tag="PK683_11685"
                     /pseudo
     CDS             complement(626..814)
                     /locus_tag="PK683_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002749959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            complement(926..1783)
                     /locus_tag="PK683_11690"
                     /pseudo
     CDS             complement(926..1783)
                     /locus_tag="PK683_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
BASE COUNT          515 a          430 c          423 g          578 t
ORIGIN      
        1 gggaactcag cgtccagctg tcacagtttc cccggacaaa ggtaggaggg ggattttcct
       61 acgtcattcc ttcctggctg attgatcttg caattccagg aaatatggaa gaactgatct
      121 ggcatttttc tcaggtactg gtacatgcca ataataaaaa gggaaccaga atgtatatga
      181 gcctcggcgc agaaggaaat tcctataagc tgaatgcatc tgcagatacg gagtttctga
      241 acaatttctt tgtgcgtttc gcactgaagg tctggaactt caaagttgct cccgacgatg
      301 aagctaaaga agatatgaaa aaactcatcg caaaaattgc aaggacagta aaggaagatc
      361 tgtccaaatt ttgattataa actaacgtca cgcaaatatg agatggtgcc ttgactgtgt
      421 aatgaatgtt cattgagtgg aatcataccg ggcaggcaaa ttcccggcaa tatttacctg
      481 attcaatgcc agcattattg caatctgttc tattcccgca aatgccaatt tgccggaatt
      541 cgaaaagatg aattcggcaa attggcttca gtgtcatctt cgttttttac gagtgcgccc
      601 agaaaaggaa ttttggcgtt cgacttcagt tgaccgtgaa ccttcctgag taacctttca
      661 tatcgtcttc gttttttacg agtgctccca gaaaaggaat tctgcttccg ctttgaagac
      721 ttacgccctg atgaatcaga atctgcatcc agtccagaag ttcgcttgtg gaaggctttt
      781 ttctgaggtc atcaagtttt cttacacggt aaaaagtgtt catcactgat tctgtaaact
      841 ctttgttcag atcaggataa tgagcagggg gaatgtcttt gagtacaaaa gagagaaccc
      901 ctaccttttc cctcgcagtt ccgatcagac ggagaccatt tatggatttc agtttttcct
      961 gagcataatc agtaagctcc ttttcatact tgtgaatatt ggaaagcccc agtttgccga
     1021 tatagtcaat cgcagctccg agtcctactg catctgcaat attcggagta cctgcctcaa
     1081 actttgcagg aggttcactg tagacagttt cttcaaatgt aacatccttt atcatatttc
     1141 ctcctccctg ccagggggga atgatctctc caagctcctt acggatgtaa acagccccta
     1201 ttcctgtcgg accgaacatc ttgtgaccgg agagaacaaa aaaatcgcat cctgattcat
     1261 tcacatttgt aggaaaatga gatacagact gtgctccgtc cacaagaaca cgggccccga
     1321 atctttttgc catctggatc atttcattta caggagggac tgttcccaga ctgttggaag
     1381 cgtttgtaat ggatacaaat ttagtcttgg gattcaggag tgatgcatat tcttccagga
     1441 tgatctctcc tctgtcgttt acaggagcga cccggatgac tgccccggtt tcctttgcta
     1501 tcatctgcca gggaacaatg tttgcatgat gttcgagcac ggtgagaaca atctcgtctc
     1561 ctgggcgaaa atattttttt ccgtatgtat ttgcaataag atttatccct tcggtggttc
     1621 ctcttacaaa tacaatttcg gaatcggatc ccgcaccgat gaatcttctg atcttgtctc
     1681 tggacttttc atatgcatca gtggatctcg cggcaagagt atgggcggcc ctgtgaatat
     1741 tcgagttgtc atgcagataa taatcggaaa tcgactggat cactgaaatc ggcttctgag
     1801 ttgttgcagc attgtcaaac cagatcagat cctttccgtt tactttctga tgaaggatgg
     1861 ggaaatcttt tctgatcaga tttacattga agatcccgct ttccagactg aacggctttt
     1921 tttcagagga atgcagaacc ctttcc
//
LOCUS       DAOQGJ010000742         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40699_length_1946_cov_1.942359, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000742 DAOQGJ010000000
VERSION     DAOQGJ010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40699_length_1946_cov_1.942359"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK683_11695"
     CDS             <1..629
                     /locus_tag="PK683_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH09155.1"
                     /translation="DLHKYSKEIVKTPGFGEKSLKILLDGIEKSKKKDFAQILPSVSL
                     SEVGHKVTEILFENGYDSLESLLKLAESKKAMEELASIHGIGEKIAESIVNSFRDKAV
                     IKLFKELEKKGLVMKTQVKKVVKGDVFAGQSWCVTGSFANFAPRENAMKIVTEYGGKS
                     VSGVSSKTTHLLHGEGAGSKLDKAKSLGVKLVDEDEFIRILEENEISY"
     gene            613..1737
                     /locus_tag="PK683_11700"
     CDS             613..1737
                     /locus_tag="PK683_11700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNH09156.1"
                     /translation="MKFLISLILFASAAACSIQKPFGKRILDVVHFSTTGDVMVHNTQ
                     IAAACRNSCTSYDFSPSFKSLPPYFAGKDFVIGNLETTLPGKKEDYTGYPLFGAPDAL
                     VKALKETGFNLLTTSNNHSLDKGKKALRRTLDVLDSFGIAHMGTYRDQNDYAKNRVLL
                     IEKKGIRFGFLSYTYGTNGIPIPKDTVVNIIDQKKIEDDISQARTLGANIIIVYFHFG
                     SEYERLPSNEQRKTVASAFKSGADIVLGGHPHVIQPYETWILTDRFGEKKKRFVMFSL
                     GNFVSSQYWRYSNGGILFEFDILKTGEGKDFSFEVSGIKYHPLYVQKQYSPVFQYNVI
                     PAKDYLDRKTDYRLNAEEEKKLTELYNDTLEHLGRYSYIQ"
     gene            1734..>1946
                     /locus_tag="PK683_11705"
     CDS             1734..>1946
                     /locus_tag="PK683_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNH09157.1"
                     /translation="MKLDPDFLKSCEKFFGDPEKAEGDLIAVGGRIYPEILFYAYSHG
                     IFPWSERPARWYSVNPRAVFDLDGLHI"
BASE COUNT          598 a          378 c          455 g          515 t
ORIGIN      
        1 atgatctgca caaatattct aaagaaatag tcaaaacgcc cggattcgga gagaagagtc
       61 tgaaaattct tctggatgga attgaaaagt ccaagaagaa agattttgca cagattcttc
      121 cttccgtgag tttgagtgag gtaggacaca aggtgacaga gattctgttt gagaacggat
      181 acgattcact ggaatctctt ctgaaactgg ctgaatccaa aaaagcaatg gaggagctcg
      241 cttcaattca cgggatcggt gaaaaaatag ccgagtcgat tgtaaactct ttcagagaca
      301 aggcagtcat aaaacttttc aaggaactgg aaaagaaggg cctcgtaatg aaaacacagg
      361 tgaaaaaggt cgtcaaggga gatgtttttg ccggacagtc ctggtgtgtg accggaagtt
      421 ttgcaaattt tgcaccgagg gaaaatgcaa tgaagattgt gactgaatat ggcggaaagt
      481 ctgtatccgg tgtctcctca aagacaactc atcttcttca cggggaagga gcaggttcta
      541 agctggataa ggcaaaatct ctgggagtga agcttgtgga tgaggatgag tttatccgga
      601 ttctggagga aaatgaaatt tcttattagt ctgattcttt ttgcttcagc ggctgcctgc
      661 tccatacaga aaccgttcgg gaaaagaata ctggacgtcg tacatttcag cacaacgggc
      721 gacgttatgg tgcacaatac ccagattgca gctgcatgca ggaactcctg cacttcctac
      781 gacttcagtc caagctttaa aagcctgcct ccctattttg caggaaagga ttttgtcatt
      841 ggaaacctgg aaactacact ccccggaaag aaagaagact atactggata tcctcttttc
      901 ggtgcaccgg atgctcttgt aaaggctctg aaagaaacag gattcaatct tctgacaact
      961 tcgaacaacc attctctcga caagggaaaa aaggctctcc gaaggacctt ggatgttctg
     1021 gattctttcg gaattgccca tatgggaaca tacagagatc agaatgatta tgcaaagaac
     1081 agagttcttc tgattgaaaa gaaaggaatc agattcggct ttctttccta cacgtatggc
     1141 acaaacggga ttccgatacc taaggataca gtggtcaata tcattgatca gaaaaaaatt
     1201 gaagacgata tatcacaggc aaggacactg ggtgctaata tcattatagt atattttcat
     1261 ttcggctctg aatatgaaag acttccttcc aatgagcaga ggaagactgt cgcatcagca
     1321 ttcaagtcag gggcagatat tgtgctggga ggacatcctc atgtaatcca gccctatgaa
     1381 acatggatac tgaccgaccg gttcggtgaa aaaaagaaaa gatttgttat gttttctctc
     1441 ggaaatttcg tttccagtca gtactggagg tattccaacg gaggaattct ttttgaattt
     1501 gacattctga agaccggaga agggaaagat ttttcatttg aagtttccgg gataaaatat
     1561 catccccttt atgtgcagaa acagtattct cctgtgtttc aatacaatgt gattccggca
     1621 aaagactatc tggacagaaa gacagactat cgcctgaatg ctgaagagga aaaaaaactc
     1681 acagagcttt ataacgatac tctggaacat ctcggaaggt acagttacat ccaatgaaac
     1741 ttgatcccga ttttctgaaa tcctgtgaaa agtttttcgg agatcctgaa aaagcggaag
     1801 gagatctgat cgcggtcgga ggcagaattt atcctgagat tcttttttac gcatattctc
     1861 acggaatctt tccctggtcg gaacgtcctg ccagatggta ctcagtcaat cccagggctg
     1921 tatttgatct ggacgggctt catatt
//
LOCUS       DAOQGJ010000743         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40753_length_1944_cov_4.032292, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000743 DAOQGJ010000000
VERSION     DAOQGJ010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40753_length_1944_cov_4.032292"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PK683_11710"
     CDS             complement(<1..1034)
                     /locus_tag="PK683_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09158.1"
                     /translation="MITRFITVGFILAGLFISSCSSRKKKILFVMPLSSLISSTHSVG
                     GTLSGLSSGSTVVLSNNGTDTKSLSADGVFSFDIRQNSETAYSVSVKTQPANAVCTVQ
                     DGTGTVAGSDISSVKVTCANSASFKIGGTVSNLSGTLILSNNGSDSLTLNTSGSYVFT
                     SPVLSGNSYSVSVQSQPTSQNCVIQNAQGTVGSSDISNIDITCTGNANGALSSGTIIN
                     TLVLTGGVTTFTGSFCAPNTVCATGSSGYADSATPSSVLFDSPSGLVTDGTYLYSADK
                     NNNVIRRISLSDGSVSTFAGSGVQGHTDGIGTSAQFDSPEFLTTDGVFLYVADAPNNC
                     IRRVVLANGQV"
     gene            complement(1110..>1944)
                     /gene="mgtE"
                     /locus_tag="PK683_11715"
     CDS             complement(1110..>1944)
                     /gene="mgtE"
                     /locus_tag="PK683_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNH09159.1"
                     /translation="IIYVVDELGMLKGCVRLKDILLASPKMKVYKLMKPVRPIHVNTH
                     QEEVAKHFRKYDLVTAPIVDDDGRMIGRITVDDVLDIVQEEATKDIYGLAGVGEEETI
                     KTSVWGSVKHRLLWLSTNLVIAMFTASIVSLFEDTIQKIVILASLMPIVAGMGGNAGT
                     QAITIMVRSLATGELTNYNWFSAVRKEFMIGIINGLTIGLITFIATYFIRFDLRISVV
                     IGLAMLINLSIAGLIGSLVPLILKFFKVDPAVASSIFVTACTDMFGFFCFLGLAAKIL
                     T"
BASE COUNT          566 a          445 c          425 g          508 t
ORIGIN      
        1 acctgaccgt ttgccagaac aactctccgg atacagttgt tcggagcatc tgctacatat
       61 agaaaaacac cgtctgtggt cagaaattca gggctgtcaa actgtgcgct ggttcctatt
      121 ccgtcagtat gtccctgcac accgcttcca gcaaaagtgg aaacagatcc gtcgctcagt
      181 gaaatcctgc ggattacatt gttattcttg tccgctgaat aaagataagt cccgtcagtc
      241 acaagccctg aaggactgtc aaacaggaca gaagagggag ttgccgaatc ggcataaccg
      301 gaggatccgg tggcacagac tgtatttgga gcacagaatg aacctgtgaa tgtggtcaca
      361 ccgcctgtca aaacaagggt attaatgata gtaccgcttg aaagtgctcc gtttgcattc
      421 cctgtgcagg tgatgtcaat gtttgaaatg tcggaactgc ccacagttcc ctgtgcattc
      481 tgaattacac agttctgact ggtcggctga gactgcacag agactgaata ggaatttccg
      541 gaaagaaccg gggaagtaaa aacataagat ccgctcgtat tcagcgtcaa tgagtcggat
      601 ccgttattag atagaatgag agttccggaa agattggaaa cagttccgcc gattttaaat
      661 gaggctgaat ttgcacaggt gactttgaca gaactgatgt cagatcctgc tacggttcct
      721 gtcccgtcct gaacagtgca tactgcgttg gcaggctgag ttttcacgga cacagaatag
      781 gcagtttcac tgttctgcct gatgtcaaaa ctgaacactc cgtcagcact gagtgatttt
      841 gtatccgttc cgttattgga aagaacaaca gttgaaccgg aagaaagacc cgaaagagtt
      901 cctccgacgg aatgagtgct gctgattaat gaagaaagag gcattacaaa aagaattttt
      961 ttcttccggg aagaacatga ggagatgaaa agcccagcaa gaataaaccc gactgtaatg
     1021 aaccttgtga tcaattttag ccgcctccgc tttatatgac agttttcctt tcagtttttc
     1081 ttaccaaaat tcattatttt acatgaaatt caggtgagaa ttttggcagc aagccccaga
     1141 aagcagaaaa aaccgaacat atccgtgcat gcagtgacaa aaattgacga tgcaacggca
     1201 ggatccactt tgaaaaactt cagaatcagg ggaacaaggg aacctatcag tcctgcaata
     1261 ctcaaattga tcagcattgc gaggccgatc actacagaga ttctcagatc gaaccgtatg
     1321 aaatatgtgg caataaatgt aatgagtcct attgtaagtc catttataat cccgatcata
     1381 aattctttcc tgacggcgga aaaccaattg tagtttgtaa gctcacctgt tgcaagactt
     1441 ctcaccataa ttgtaattgc ctgagttcct gcatttcctc ccattccggc tacaataggc
     1501 atcaaagatg caagaatgac tattttctgt atcgtgtcct caaaaagaga tacaatcgat
     1561 gcagtgaaca ttgctattac aagattcgtg gaaagccaga gaagtctgtg tttgactgaa
     1621 ccccagacag aagtcttgat cgtctcttcc tctcccactc cggcaagccc gtaaatatcc
     1681 tttgtcgctt cttcctgaac aatgtccagg acatcgtcaa cagtaattct tccgatcatc
     1741 cgtccatcgt catccacaat gggagcggtc accagatcat acttcctgaa atgtttggca
     1801 acttcctcct gatgagtgtt cacatgaata ggtctgaccg gcttcatcag cttgtagact
     1861 ttcattttgg gagaagcaag caggatatcc ttcagacgga cacagccttt cagcattccc
     1921 agttcgtcca cgacatatat tata
//
LOCUS       DAOQGJ010000744         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40771_length_1944_cov_2.392800, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000744 DAOQGJ010000000
VERSION     DAOQGJ010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40771_length_1944_cov_2.392800"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PK683_11720"
     CDS             <1..378
                     /locus_tag="PK683_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH09160.1"
                     /translation="VLSGKYLEGETGRVKEGSPRLNERAVKIAKKTAEIAKSKNCTPT
                     QVAVRWVMQKNRINVPIIGARKLSQLEDSLTSADIELNENEMKELNEVSGIELGFPHD
                     FIRSAGPQNVMYGDQKKFFDFQS"
     gene            complement(369..689)
                     /locus_tag="PK683_11725"
     CDS             complement(369..689)
                     /locus_tag="PK683_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNH09161.1"
                     /translation="MEEMNQLEADIYNSLRTVEDPELHIPIVDLGLIYDVKADAEGKA
                     LIKMTLTSMGCPAGPYLKAETISAALKVNGVTDADVEIVWTPKWDPRLMASEDAKEML
                     GIYD"
     gene            complement(690..1112)
                     /locus_tag="PK683_11730"
     CDS             complement(690..1112)
                     /locus_tag="PK683_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNH09162.1"
                     /translation="MSLKEDLYKEVILDHYENPRNYGVLNPCQRHEKGANPLCGDELE
                     LFLNFSGDVIESITFHGKGCSISQSSGSMMTELVKGKTKEEALAVMKKFKLAILEDKE
                     QEFTEEEGDLESLLGVKKYPVRVKCAVLAWNTLERALN"
     gene            complement(1087..>1944)
                     /locus_tag="PK683_11735"
     CDS             complement(1087..>1944)
                     /locus_tag="PK683_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017853437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH09163.1"
                     /translation="MLAEEKNAKIKYIPIREDATLDLSRLDEIITDRTNLVAVSQMSN
                     AVGTIHDIEPIIKKAHSVGAVFLLDGAQGACHLKTDVEKLGVDFYAFSAHKMLGPTGV
                     GVLYGKEEILEKMPPWMGGGDMISDVSLQRTTYADLPARLEAGTPNIAGVISFRAAVQ
                     YLEKIGLEEIHKHEMSLVEYALGRLEELGGIKLYGPKEIEKRGGVVSFNVDGVHAHDV
                     GSILDEEGIAIRVGHHCCQPYMKKNGIAGTCRASFYLYNTKEDVDSLIQGVKKVKEIF
                     SRVTQRRSV"
BASE COUNT          503 a          479 c          395 g          567 t
ORIGIN      
        1 gttctttcag gaaaatatct ggaaggagag actggaagag tgaaggaagg aagtccgcgt
       61 ctgaatgaaa gagcagtgaa aattgcaaaa aaaacagcgg agattgcaaa aagtaaaaac
      121 tgtaccccga cccaggtcgc agtcagatgg gtgatgcaga aaaaccggat caatgtaccg
      181 atcattgggg caagaaaact ctcccagctc gaagactcat taacgtctgc agatattgag
      241 ctgaatgaaa atgaaatgaa ggaactgaat gaggtaagcg gaatagaact gggattcccg
      301 cacgatttta tcagatcggc aggacctcag aatgtaatgt acggagatca gaagaaattt
      361 ttcgactttc agtcgtaaat gccgagcatt tccttggcat cttcactggc catcagtctt
      421 ggatcccatt tcggggtcca gacaatctcc acgtccgcat ctgtgacacc gttgactttc
      481 agagctgcag aaattgtttc tgccttgaga tatggacctg caggacagcc catacttgtg
      541 agagtcattt taatgagtgc ctttccttct gcatctgctt tcacatcgta gatgagtccc
      601 aaatccacta tagggatatg cagttcagga tcttccactg tcctgagtga attataaata
      661 tctgcttcca gctgattcat ttcttccatt cagttcaatg ccctctcaag agtattccat
      721 gcgagaaccg cacattttac gcggacagga tattttttga ctccgagaag tgattccaga
      781 tcaccctctt cttccgtaaa ctcctgttcc ttgtcttcaa ggattgccaa tttgaatttt
      841 ttcattacag ccagtgcttc ttcctttgtc tttcctttga ccagttctgt catcattgag
      901 ccggaagact gagatatgga acagcctttt ccatgaaatg ttatgctttc aatcacatct
      961 ccgctgaaat tcagaaacag ctcaagctcg tcaccgcaga gagggttggc tcccttttca
     1021 tgtctctgac acggattcaa tacaccgtaa tttctcgggt tttcataatg atctaagatg
     1081 acttccttat acagatcttc tttgagtgac acggctgaat atctccttca ctttctttac
     1141 gccctgaatc aaggaatcca catcctcttt cgtattgtaa agataaaaac ttgccctgca
     1201 ggttcctgca attccgtttt ttttcatata gggctgacag cagtggtgac ccaccctgat
     1261 tgcaatccct tcctcatcca gaatggaacc tacatcatga gcatgaacgc cgtccacatt
     1321 gaaggaaact acaccgcctc ttttttcaat ttccttaggt ccgtaaagtt taattccccc
     1381 aagctcttcg agtcttccaa gtgcgtattc cactagactc atctcgtgct tatgaatttc
     1441 ctccagtccg attttctcga gatactgaac tgccgctctg aaggagatga ctcccgcaat
     1501 attcggagtg cctgcttcca gtctggcagg aagatcggca tacgttgttc tctgaagact
     1561 gacatcggag atcatatcac ctcctcccat ccagggaggc attttttcca ggatttcttc
     1621 ttttccatac agaactccca ctcctgtagg tccaagcatc ttgtgggctg aaaacgcata
     1681 gaagtccaca cccagttttt ccacatccgt cttcagatga catgccccct gagctccgtc
     1741 cagaagaaag actgcaccga cagagtgtgc ttttttaatg ataggttcta tatcgtgtat
     1801 tgtccctact gcattcgaca tctgggacac tgccacaagg ttagtcctgt ccgtgatgat
     1861 ctcatcgagc ctggaaagat ccagagtggc atcttctctg atcggaatgt attttatctt
     1921 cgcatttttt tcttctgcaa gcat
//
LOCUS       DAOQGJ010000745         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40785_length_1943_cov_3.398305, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000745 DAOQGJ010000000
VERSION     DAOQGJ010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40785_length_1943_cov_3.398305"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1233)
                     /locus_tag="PK683_11740"
     CDS             complement(151..1233)
                     /locus_tag="PK683_11740"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH09164.1"
                     /translation="MESAGNPAKNTPDMGVKTGKKGGRKAKNSPSLSLNQIQKKKEIL
                     EKILKFGSNRERKEAMRELLHFPKEHSQELYQLVSEILANDTDLGIRIVCLRTLGEIG
                     YTKEPPVIINLLSEKSDDIKEAAVFAIQKLKLDEASPEIVKLLKEQDFSKNMTLVNNS
                     VTALGELQSGKVGGDFLESKFRDKTTHANVRSNIALYFSKTKDTRAEAALIDAASDEG
                     EDQTTRAYAVNALGKMNSHKSIPAIREILSKINDAKGKFEVKKLAHLKIYCISALVAL
                     GDSEVMKELVSYAKDDDPNIRIRAIKQLAEFDTKEMREMLEYKAQRDPSKKVQETAKK
                     LLEELKKKEADSEGSKAVNPETPAKK"
     gene            complement(1382..1849)
                     /locus_tag="PK683_11745"
     CDS             complement(1382..1849)
                     /locus_tag="PK683_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH09165.1"
                     /translation="MSAGKGYIICVDDEVAVLETLQEQLRERFSASHEIEIAASAENA
                     LSLIEEILDAGEVIEVVISDQVMPGMKGDQFLEQIHRRLPEAIKILLTGQASLEATIR
                     AINYGGLSKYVEKPWNIDLLARDIHELISKFKQNIENQHLIKELNKRIKELEK"
BASE COUNT          475 a          441 c          382 g          645 t
ORIGIN      
        1 gacatttgta aaacagcttc ctttcgggag gaaagctttt ttcctttctg tagtcattca
       61 ctcttttttc atgaatgctg atatcactct gatatttctc ctccgagagg agggagaaca
      121 tgcagaaaaa tgaaattata atgaaaactt tcattttttt gcaggagttt cgggattgac
      181 tgctttcgaa ccttcagaat ctgcttcctt cttcttgagt tcttccagaa gttttttggc
      241 agtttcctga accttcttgc tcggatctct ctgagccttg tattccagca tttcccgcat
      301 ttcctttgta tcgaattcag cgagctgctt gattgcacgg attctgatgt taggatcatc
      361 atcctttgca taactgacaa gctctttcat cacttcagaa tcaccaagtg ccacaagcgc
      421 actgatgcag tatattttca ggtgagcaag tttttttact tcaaacttgc cctttgcatc
      481 attgatcttg ctcagtattt cacggattgc cgggatagat ttatgggaat tcattttccc
      541 aagggcattc actgcatagg cacgggttgt ctgatcctca ccctcatcgg acgcggcatc
      601 aatcagagcc gcctcggccc ttgtatcctt agtcttgctg aaatacagtg caatattcga
      661 acggacattt gcatgagtcg tcttgtccct gaatttggat tccagaaagt ccccgcccac
      721 tttccctgac tgaagttctc cgagggctgt caccgaattg ttgactaaag tcatattctt
      781 tgaaaaatcc tgttctttca gaagtttcac aatttcagga ctggcctcat ccagcttcag
      841 tttctgtatt gcaaaaaccg ctgcttcctt gatgtcatcc gatttttcag aaagaagatt
      901 gatgattaca gggggctctt tggtataacc gatctctccc aatgtcctga ggcagacaat
      961 tcgtattccc agatccgtat cgtttgcgag gatttcggaa acaagctggt acagctcctg
     1021 agaatgttcc tttggaaaat gaagaagctc acgcattgct tcctttcttt ccctgttgct
     1081 cccgaatttc aggatttttt cgagtatttc ttttttcttc tggatctggt tcaggcttaa
     1141 ggaaggtgag tttttggctt tccgaccgcc ttttttacct gtctttactc ccatatccgg
     1201 ggtattcttg gcaggatttc ctgcactttc catattaccg acctgttcag gggattcctc
     1261 cgaaggagtc tgagaagaat ctgtcaaaac aagatcaggt acaggagcaa tttttttggt
     1321 ttctttggaa aaaagggaag caggaagaaa gagtaaggat ataagaatca gaaaagtatg
     1381 tctacttttc caattctttg attcttttgt tgagctcttt gataagatgc tggttttcaa
     1441 tgttctgttt gaatttggaa ataagttcat gtatgtctct cgccagtagg tcaatgttcc
     1501 aaggtttctc tacgtacttg cttagacccc cgtaattgat tgcccgaatg gttgcttcca
     1561 gacttgcctg tcctgtgagg agaattttta tcgcttcagg gagccttctg tgaatctgct
     1621 ccaaaaattg atctcctttc attccaggca taacctgatc tgaaataaca acttctatga
     1681 cttcgcctgc atcaagaatt tcttcaataa gcgacagagc attctcagcc gacgccgcga
     1741 tttcaatctc gtggctcgca ctgaagcgtt ctctgagctg ctcctgaaga gtctcaagca
     1801 ctgctacttc atcatcgaca caaattatat aacctttacc agctgacata gcaaattgaa
     1861 ttatacctac agcaccattt ttggcaatag ggaaatcttt ccaataacta atttaatcag
     1921 aaccggcatc agaaatattt ttt
//
LOCUS       DAOQGJ010000746         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40820_length_1942_cov_2.963434, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000746 DAOQGJ010000000
VERSION     DAOQGJ010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40820_length_1942_cov_2.963434"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PK683_11750"
     CDS             <1..749
                     /locus_tag="PK683_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNH09166.1"
                     /translation="TYMAKNRIMSHSENELRDPSDRVRAACGSDVEKCMKKFEQSISM
                     PGSYHVCCGENVITSLTSNSAGISYFIRTDSQGTYKEWKNADIRWYDESGLAKDMVSR
                     WMKSPGHRANILNPEYNSMGAAADFNAETEYYYGTQVFSPYADGAFDYDPFSVSLEQK
                     ENHNPGIRIDPGKKLSNTSVRIYLGEKLQETEKQGQTYLLSLPKDIQKEKIISIEVKD
                     RARDYWYPYWQIRIFSKDEKMQWEWKRWEY"
     gene            798..1331
                     /locus_tag="PK683_11755"
     CDS             798..1331
                     /locus_tag="PK683_11755"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNH09167.1"
                     /translation="MSIIKSKIRTIPDWPKPGIMFRDITSLLIDPEGLALTIGTFVAR
                     YQNAGITKVAGIEARGFITGAAVAFQLNVGFVPVRKKGKLPGETVSEEYALEYGTDMV
                     EIHKDAITPNDNVLIMDDLIATGGTLGAAIKLIRKLGGKVHEAAMIIDLPELGGSKKI
                     TSEFGVGVYSICEFEGH"
     gene            complement(1328..>1942)
                     /locus_tag="PK683_11760"
     CDS             complement(1328..>1942)
                     /locus_tag="PK683_11760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH09168.1"
                     /translation="SLITVLVFLYNFYLLDIFTPLSPGIDILYIDTLFLLLIVAALVQ
                     FKFILFYPGFLSLVVYGELPLLVIHNQAPANQAGTAYLKDELWRIYEVESWSTFISEF
                     WYGIIIDETLDNALEHGGKRFEDKITIQVYETSKYLDFYVIDMGKGFDPETLPNPADP
                     NRKNIPTGRGIYIMRKLFQVDWNFLGNEIRVRVSKNPADNPSDI"
BASE COUNT          632 a          379 c          448 g          483 t
ORIGIN      
        1 gcacctatat ggcaaagaac agaatcatgt cgcacagcga aaatgagctc agagacccgt
       61 cggacagggt aagagcagcc tgcggatccg atgttgaaaa atgcatgaaa aaattcgaac
      121 agtcgatttc aatgcccgga agttatcacg tctgctgcgg agaaaatgtg atcacaagtc
      181 tgacaagcaa ttcagcagga atttcttatt ttatcagaac tgacagtcaa ggcacttaca
      241 aagaatggaa aaatgcagac atcagatggt atgatgaatc cgggctggca aaggatatgg
      301 tttcgagatg gatgaaaagt cctggtcaca gagcaaatat cctcaaccca gagtacaatt
      361 cgatgggggc cgctgcagat tttaatgcgg agacagaata ctattacgga actcaggtgt
      421 tctcccctta cgcagacggg gcctttgact acgatccgtt ttcagtaagt cttgaacaga
      481 aagagaatca taatcccggg atcaggattg atcctggaaa gaaactgtcc aacacatcgg
      541 tacgtattta tttgggagag aagctgcagg aaactgaaaa acagggacag acttatctgc
      601 tcagtctgcc taaggatatt caaaaggaaa aaatcatctc gattgaagtc aaggacagag
      661 ccagagacta ctggtatcct tactggcaga ttcgtatatt ttcaaaagat gaaaaaatgc
      721 aatgggaatg gaaacgctgg gagtactgat ttttaaaagg actttacagg agaagtgcaa
      781 aaaattacat ctgccttatg tctattatta aatccaaaat cagaaccatc ccagactggc
      841 cgaaaccagg tatcatgttc agagatatta cttcgctgtt gatcgatccg gaaggattgg
      901 ctctgacaat tggaactttc gttgccagat atcagaatgc agggattaca aaggtggcag
      961 gcattgaggc cagaggattc attaccggag ctgctgtggc tttccagctg aatgtaggtt
     1021 ttgtacctgt cagaaaaaaa ggaaaacttc ccggagaaac tgtatcagaa gaatatgcac
     1081 tcgaatatgg tactgatatg gtggaaattc ataaggatgc gattactcca aatgacaatg
     1141 tactgattat ggatgatctg attgccactg gcgggacttt aggcgctgcg attaaactca
     1201 tcagaaaact tggcggaaaa gtacatgagg cagcgatgat cattgatctt cctgaactgg
     1261 gcgggagcaa aaagatcact tcagaattcg gtgtaggagt ttattccatc tgcgaatttg
     1321 aaggtcatta aatatcgctg ggattgtcag cagggttctt ggaaacacgg accctgatct
     1381 catttcctaa aaagttccag tctacctgga atagttttct cataatgtaa atcccgcgtc
     1441 ctgtaggaat atttttcctg ttgggatcgg ccggattggg aagagtttca ggatcaaaac
     1501 ctttccccat atcaatcaca taaaaatcca ggtatttgga tgtttcatac acctgtattg
     1561 taatcttgtc ttcaaaacgt tttccaccat gctccagtgc attgtctaac gtttcatcaa
     1621 taataattcc gtaccagaat tcgctgataa aagtagacca gctttccact tcgtagattc
     1681 tccaaagctc atccttcaga taagctgttc ccgcctgatt ggcaggggcc tggttatgaa
     1741 tgaccagaag aggaagttca ccgtatacaa ccagagaaag aaaaccagga taaaacagga
     1801 taaacttaaa ctgaaccagt gcggctacaa tcagcagtaa aaagagagtg tctatataaa
     1861 gaatgtcaat ccctggagaa agcggagtaa aaatatccag cagataaaaa ttgtacaaaa
     1921 agaccaggac ggtgatcagg ga
//
LOCUS       DAOQGJ010000747         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40858_length_1941_cov_2.910392, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000747 DAOQGJ010000000
VERSION     DAOQGJ010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40858_length_1941_cov_2.910392"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PK683_11765"
     CDS             <1..710
                     /locus_tag="PK683_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH09169.1"
                     /translation="KTAKEAAEKANRAKTEFLSGMSHELRTPLNAIIGFTDLIRAGGL
                     SEDQRVLSEYVLNSANHLLKLIDNLMNFPGIEAGSADSDSSFKKQEVQSVPEKNVQSP
                     QKSSYEKMRGCVLYVEDNVLNSALMNRIFQRNLPNVELVISKDAEEGLETADRIHPDL
                     ILMDIHLPGMNGFQAASSLKAAETTSDIPVIAVSADATPTALKNAEESGIIAYITKPY
                     NAQDLLRKVTVFLKTAV"
     gene            1044..1742
                     /locus_tag="PK683_11770"
     CDS             1044..1742
                     /locus_tag="PK683_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH09170.1"
                     /translation="MQVPKNGYQVLVVDDEEDILDLIKFNLEEEGFLVSVSKNGLEVL
                     PKMQKQTPDLLILDVMLPGIGGIELCKKIKEKYPTPIIMVTAKTGETDAVLGLELGAD
                     DYVRKPFSPRELIARVKSVLRRYSKEDTDSSKSANISIGKIYLNSKAHKVYVDGKEVD
                     LTLLEYKILHFFMSNPGTALSREKLLDSVWGRDIYVTDRAVDVNVKRLRTKLGVEKNR
                     LETVRGIGYRFHDA"
     gene            1735..>1941
                     /locus_tag="PK683_11775"
     CDS             1735..>1941
                     /locus_tag="PK683_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HNH09171.1"
                     /translation="MRSFFSRLLVMNWGFLTILTLAVLTVFYFSTNLLPELQFLMFMI
                     VWIFAFFGTFHISYKISENVTETLR"
BASE COUNT          579 a          373 c          447 g          542 t
ORIGIN      
        1 tgaagacagc aaaggaagct gcagaaaaag cgaacagggc gaagacggaa tttctttccg
       61 gaatgagtca cgaacttaga actcctctga atgcaatcat cggatttact gatcttatcc
      121 gtgcaggcgg cctttctgaa gatcagaggg ttctctccga atatgtcctg aacagtgcaa
      181 atcatctgct taagctgatt gataatctta tgaattttcc aggaatagag gcaggcagtg
      241 ctgattcaga ttcttcattc aaaaagcagg aagtccagtc tgtaccggaa aaaaatgtcc
      301 agagtcccca gaaatcttct tatgaaaaaa tgagaggctg tgttctgtat gtggaagaca
      361 atgtactgaa ttcagcactg atgaacagaa tatttcagag aaatcttcca aatgtagaac
      421 ttgtcatttc caaggatgca gaagaaggtc tggaaaccgc tgacagaatc catcctgatc
      481 tcatcctgat ggatattcac cttcctggaa tgaacggttt tcaggctgct tccagcctca
      541 aggcggcaga gacaacctcg gacattcctg tcattgcagt cagtgctgat gcaactccga
      601 ctgcactgaa aaatgccgaa gaatcaggaa taatcgccta cattacaaaa ccgtacaatg
      661 cacaggacct tttaaggaaa gttacagtat tcctgaaaac agctgtctga aattttttta
      721 taaaatccgc aataagtcat ttctctgtaa tgagtttgta acaattcggc tgtataagaa
      781 tacagtaagt attgagtaag ataaagttat tctcaatttg agtttagccc tgcggtttat
      841 gcatacatag aagcagaatt tttccgcagg gatgatctta tctcagtaca gccttatttc
      901 agtgcagtaa gactgctttt tgaagcgggg ttgggggcaa gcccctgaat ccgcagagac
      961 tgccttttcc tgctttttta aaagcaggca gtgaactgag gttttattcg agttcacatt
     1021 attatttgtg ctggaggagt actttgcagg ttcccaaaaa cggttatcag gtactggtag
     1081 tggacgatga ggaagatatt ttagatctga tcaaatttaa tttagaggaa gaaggttttc
     1141 ttgtttccgt cagtaaaaac ggactggagg tccttcctaa aatgcagaag cagactcctg
     1201 accttctgat tctggatgta atgcttccag ggatcggcgg aattgagctc tgtaaaaaaa
     1261 taaaggaaaa atatccgact ccaatcataa tggtgactgc aaaaacaggt gagacagatg
     1321 cggttctggg ccttgagctc ggggcagacg actatgtcag aaaacctttc agtccaagag
     1381 agctgattgc ccgcgtcaag tccgtcctga gaagatactc taaggaagat actgattcct
     1441 ccaagtctgc aaatatttcc atcgggaaaa tatacctgaa cagcaaggct cataaggttt
     1501 atgtggacgg caaggaagtg gatctgactc ttctggaata caagattctt cattttttta
     1561 tgagcaatcc tggaactgct ctgagcagag agaaacttct ggacagtgtc tggggcaggg
     1621 atatctatgt gactgaccgg gcagtggacg tgaatgtaaa aagactcaga acaaaacttg
     1681 gagtcgagaa gaacagactg gagactgtca gaggaatagg ataccgtttt cacgatgcgt
     1741 agtttttttt cgagacttct ggtaatgaac tgggggtttc tgacaatcct gacacttgca
     1801 gttctgactg tcttttattt cagcacaaat ctgcttccgg aacttcagtt tctgatgttc
     1861 atgattgtat ggatctttgc attttttggt acatttcata tctcatataa aatttcagag
     1921 aatgtcacgg aaacacttag g
//
LOCUS       DAOQGJ010000748         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_40870_length_1941_cov_1.983033, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000748 DAOQGJ010000000
VERSION     DAOQGJ010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40870_length_1941_cov_1.983033"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PK683_11780"
     CDS             <1..821
                     /locus_tag="PK683_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH09172.1"
                     /translation="GPWRFGIVVRYSADYRASIKSIEDIPIIAPGGERIPLSELAKIG
                     LENGATLIFRQEGRRNITVRTNIRGRDQGGFVAEAQKRVAEKVQLPPGYFIKWGGQFE
                     NLSRVGKQLAIVIPLTIAIIFGVLYLLYNNIRHVLVAMSCLPFSLIGGMVALLIRGYY
                     FNVSAGIGFISLFGVATISGVLFVSRTNLILAEEEDLTIEQAARKAATIQFRPLMMTM
                     MLALLGLIPAMFGTGVGSDVQRPLATVIVGGLTSGLILMLHVLPSLYIVTVKKD"
     gene            909..1190
                     /locus_tag="PK683_11785"
     CDS             909..1190
                     /locus_tag="PK683_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09173.1"
                     /translation="MKTIETIGAVQDDNCLILKLSGDILPGRYRIVLVLEEEPIEEEL
                     TEEELTEEEKSYFDRELKAIKENPELGQSWADIVNELEIEIGRKIPVHR"
     gene            1249..1449
                     /locus_tag="PK683_11790"
                     /pseudo
     CDS             1249..1449
                     /locus_tag="PK683_11790"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1562..>1941)
                     /locus_tag="PK683_11795"
     CDS             complement(1562..>1941)
                     /locus_tag="PK683_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09174.1"
                     /translation="MLILNVIEFWTDSNPLGLNEYDKDGRFVRELAEGDSKVRLEYTG
                     FGQRLNISLSEKDKLSSFVVLRNQPGEIFEEREGRLIPINVESQKIGNKAVLRMVKNG
                     KLESAKVVDYEKISEIESKLSVY"
BASE COUNT          573 a          374 c          431 g          563 t
ORIGIN      
        1 aaggtccctg gaggttcgga attgtagttc ggtattctgc agactacagg gcatcgatta
       61 aatccattga agacatccct atcattgcac cgggaggaga aaggatacct ctctctgaac
      121 ttgcaaagat aggtcttgaa aacggtgcaa ctctgatctt ccgtcaggaa ggaagaagga
      181 acattacagt caggacaaat atcagaggaa gggaccaggg aggttttgta gcagaagcac
      241 agaaaagagt tgctgaaaaa gttcagctcc cgcccggcta cttcatcaaa tggggaggtc
      301 agttcgaaaa tctgagccgt gtaggaaagc agcttgcgat agtaattccg cttacgattg
      361 ccataatatt cggtgtactc tatcttcttt acaacaatat tcggcatgtt cttgtggcca
      421 tgtcctgtct tccgttctct cttatcggcg gaatggtggc acttcttatc aggggttact
      481 actttaacgt atccgcaggg attggattca tttcgctatt cggagtggca acaatttccg
      541 gagtgctctt tgtatcgaga acaaatctga ttctggctga ggaagaagat ctgacaattg
      601 aacaggctgc aagaaaggct gcaacgattc agttcagacc cctgatgatg acgatgatgc
      661 tcgccctgct cggtctgatt ccggcgatgt tcggaaccgg tgtgggttca gatgttcaga
      721 gacctctggc cacagtcatt gtagggggac tgacttcagg actgatactg atgcttcacg
      781 ttctgccttc tctgtacatt gtgacagtaa agaaagactg attacgtatc aacgcaccct
      841 gagctttcaa agggtgcgtt tggaaaaatg tttgcataca tttacaaatg ttttatcctt
      901 tgtcatatat gaaaacaata gaaaccattg gtgctgttca ggatgacaac tgtctgattt
      961 tgaaactgtc aggcgatatt cttcccggcc gttatcgaat cgttctagtt ctggaagaag
     1021 aaccgattga agaagagttg actgaagaag agttgactga agaagagaag tcctattttg
     1081 acagagaatt gaaggcaata aaagaaaacc cggaattagg tcagtcttgg gcagacatag
     1141 tgaatgaatt ggagatagag attggcagaa aaataccagt tcacagataa tgcaaagaaa
     1201 cagctaaaag aaaattttaa gtaatataat gataaaaatc cgaatgccgc tgatgaattt
     1261 atagaagacg tttatgaaaa agtgaatcaa attgcgaata aaccggaatc tcattctatc
     1321 gtttatgagg atgtccgcaa ggcatcttta agaaaatttc cttttttcct ttattatgca
     1381 ttcagaaata ctatagtgta tatccttgct gtctggcaca aaaaaagaga acagaggatt
     1441 atggaataac agtttgattg gctgaagaat aatttattta ttcagatctg atagaatttc
     1501 gctggagtgt tgtcaaatgt gcgccttctg cttgaggcag aaacgcacat acattcattt
     1561 ttcagtaaac ggaaagtttg gattctattt cagaaatttt ttcgtaatcc actactttcg
     1621 cagattccag tttgccattc ttgaccattc tcagaacagc tttgtttcca attttctgag
     1681 actctacatt gatcggaatc agtctgcctt ctctttcttc aaaaatttca cccggctggt
     1741 ttcttaaaac aacgaatgaa gacagtttgt ctttttcaga cagacttata ttgagtctct
     1801 gaccgaatcc ggtatactcc agtcggactt tggagtcccc ttcagccagt tctctcacaa
     1861 aacgtccgtc cttgtcatat tcattcagtc caagaggatt ggagtcggtc cagaattcaa
     1921 tgacattaag aataagcata t
//
LOCUS       DAOQGJ010000749         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41009_length_1937_cov_3.527630, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000749 DAOQGJ010000000
VERSION     DAOQGJ010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41009_length_1937_cov_3.527630"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..843
                     /locus_tag="PK683_11800"
     CDS             70..843
                     /locus_tag="PK683_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNH09175.1"
                     /translation="MIIKLFYLTNFGNVRTSNEDAILLHDSLIINRNMRKPESRIIEN
                     SSIVLCVADGMGGHVKGEMASATVLETVQNSLPEMLTHKTVKKVILESRKKLNRVAIS
                     ENAYGLGTTLTGLSFHKKKALLFNCGDSRVYRAGSNFLEKLTNDHSLVQGLYDSGLID
                     EEGMRNHPNKNILTSAIIGDLSDEEPKITIRTIDLKIGSMFLLCTDGLWESLNTDELI
                     QCFDPALKPEEISEKLLKESIEKGGRDNISYILAQIIDI"
     gene            856..1902
                     /locus_tag="PK683_11805"
     CDS             856..1902
                     /locus_tag="PK683_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH09176.1"
                     /translation="MKKTLLFSALFISTILLLNGETSMNASKPPHHADKGFRNLSPDA
                     QMRNLLDVIRWQTENIFKKLPSRNPKDYSFTVYSNDGYELKKNREQFSATWVGHATVL
                     IQIDGKNILTDPIWSERCSPVGFAGPKRYTAPGIDIDRLPKIDAVVISHNHYDHMDIP
                     TLKVLDKKFSPVIYAGLRNREFLKSEGLKNPVELDWWQSDESAGIKITFTPTQHFSGR
                     GVLDHGETLWGSYVIEGKTKKAYFAGDTAYFSGFAEIGNKFPKIDLAILPIGAYEPRW
                     FMKPVHMNPEESVKTFLDLKADNMLPMHYQTFVLTDEPLDEPLRLTKELFEKNGISLT
                     RLADLKIGESRFWK"
BASE COUNT          630 a          391 c          424 g          492 t
ORIGIN      
        1 tgcttaccaa acattcagaa ttccttttct accctaaaat cggctcgaaa cagagattca
       61 ggttgtaaga tgatcattaa actattctat ctgacaaatt tcggaaatgt acgtacatca
      121 aatgaagacg caattcttct gcatgattcg cttatcatta atagaaatat gcgcaagccg
      181 gaatccagaa tcattgaaaa cagttcgatc gtgctctgtg tcgctgacgg gatgggcgga
      241 catgtgaagg gagaaatggc aagtgcgact gttctcgaaa ctgtccagaa cagtcttccg
      301 gaaatgctca ctcacaagac agtaaaaaaa gttatattgg aatccagaaa aaaactgaac
      361 agagtggcta tatctgaaaa tgcatacggt ctgggaacaa ctctgacagg cctgtccttt
      421 cataaaaaaa aggcgctcct tttcaactgc ggtgacagca gggtttacag agccggatca
      481 aactttctgg aaaagctgac aaatgatcac tctcttgttc agggtctgta tgattcaggg
      541 ctcattgatg aagagggaat gagaaaccat cctaataaaa atattcttac ctctgccatc
      601 atcggagatc tgagtgatga agaaccgaaa ataacaattc gcacaatcga tctcaaaatc
      661 ggctctatgt tcctgctctg cactgacgga ctctgggaaa gcctgaacac ggatgaactc
      721 attcagtgtt ttgatcctgc tctgaaaccg gaagaaattt ccgaaaaact tctgaaggaa
      781 tccattgaaa agggaggcag ggacaatatc agttatattc ttgctcagat aatagatatt
      841 taataaaagg gagttatgaa aaagactctg cttttttcgg cattatttat ttccacaatc
      901 cttcttttga acggagaaac ttccatgaat gcatcaaaac ctccccatca tgcggacaaa
      961 ggattcagaa atctttcacc ggatgctcag atgagaaatc tgcttgatgt aatcagatgg
     1021 cagacagaga acatattcaa aaaacttcca agcagaaatc ctaaagacta ttcatttaca
     1081 gtgtattcca atgacggata cgaacttaag aaaaacaggg aacagttttc agcaacctgg
     1141 gtaggacatg caacggtgct catacagatt gacggaaaaa acattttgac tgatccgatc
     1201 tggtcggaaa gatgctctcc ggtcggcttt gccggaccta aaagatatac tgcaccgggg
     1261 atagacattg acaggcttcc caaaatagat gcagtagtca tcagtcacaa ccattatgac
     1321 catatggaca tccctactct aaaagttctt gacaaaaagt tttcgccggt catttacgcg
     1381 ggactgagaa acagggagtt tctgaagtcg gaaggactga aaaatccggt ggagctggac
     1441 tggtggcagt cggatgaatc tgcaggaatc aaaattacat ttacgcctac tcagcatttc
     1501 agcgggagag gagttctgga tcacggagaa actctctggg gaagttatgt cattgaaggg
     1561 aagacaaaaa aggcatactt tgcaggggac accgcctatt tcagcggatt tgccgagata
     1621 ggaaataaat ttccaaagat tgatctggcc attcttccca taggcgcata tgaaccgaga
     1681 tggtttatga aaccggtaca tatgaatcct gaagagtctg taaagacatt tcttgacctg
     1741 aaggcggaca atatgcttcc tatgcattat cagacgtttg tgctgaccga tgaacccctg
     1801 gatgaacctc tcagactgac aaaggaactc tttgaaaaaa acgggatcag tcttacaaga
     1861 ctggctgatt tgaaaatcgg agaatccagg ttctggaaat aatgcagaaa tctgaatgat
     1921 tttttattcc gaaacat
//
LOCUS       DAOQGJ010000750         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41069_length_1936_cov_2.211590, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000750 DAOQGJ010000000
VERSION     DAOQGJ010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41069_length_1936_cov_2.211590"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PK683_11810"
     CDS             <1..813
                     /locus_tag="PK683_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH09177.1"
                     /translation="PFEKVAAGTLVVKILGGSAFRSPTFQELYDKTQQFQQGGILGND
                     QLKPETIRTAEIGADYRTPYAPLGIMLNGYYNRILNNIEGFNQSGVYPGQTDIYANLR
                     GISIFGYEAEIRLNYSARNYGFINGYWTQVLDYGGLPPNIKKDTTTFRLDVPQARLNL
                     GINFEFTKYFVMNHTVWVTSERASNSRYSYESQDSKVFRIPQYYIWNLSLRTTEDLHR
                     NIFFQASIYNMNNYKLYDEINVPTASFYPNRVIPSSWLWGRYFEFKLTYLLD"
     gene            822..1877
                     /locus_tag="PK683_11815"
     CDS             822..1877
                     /locus_tag="PK683_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNH09178.1"
                     /translation="MKFFISLIQKFTERDSSDLFFHYSVMMIFFFSAFCIQKKSPPSS
                     AAAAGSLVTNTVSLGAPKSIGLDLVTNIKAWNRGCELRTGNLVADAFAWKANADIGFV
                     NGGNIREDQNVPTILKGTVPDSTLFAKFMIFNNQVIVSEINGYRLKQALENSNRQLTL
                     PRYSSATDDMDSDGSQHGNCYSFGSGSGRILFPSSNLSVDVNVSNTAMSVSGSAGSNN
                     LKTNTVGARITRIVINGLMIYNNTSGSFTSGWAAGTSSCTVRQTSFTNSAACNKYKIA
                     TVDFQINGGDGNFSFNPSITANAAGPTEFQGDVPMSAAVINTPIGTDSGIAYEYVQSF
                     TSGPVFPKIGNRLNLFH"
BASE COUNT          594 a          415 c          400 g          527 t
ORIGIN      
        1 ccttttgaga aggtcgcagc aggaactctg gttgtaaaaa ttctcggagg ttcagcattc
       61 cgctccccta cttttcagga actgtacgac aagactcagc agttccagca aggcgggata
      121 ctgggtaatg atcaattgaa accggaaaca atcagaacag cagaaatcgg agctgactac
      181 agaactccat acgcaccttt gggaattatg ctgaacggat attataaccg gatactgaac
      241 aacattgaag gattcaatca gtcaggagta taccctggtc agacagacat ctatgcaaat
      301 cttcgaggca tctccatttt cggctacgag gcagagatac ggctgaatta ttccgcaaga
      361 aattacggat tcatcaacgg atactggaca caggtgcttg actatggagg acttcctcca
      421 aatataaaaa aagataccac gactttccga ctggatgttc cgcaggcgcg tctgaacctg
      481 ggaataaatt ttgaatttac aaaatatttt gtaatgaacc atactgtatg ggtaacatca
      541 gaaagggcat ctaacagcag gtacagttat gaatcccagg acagcaaagt tttcagaata
      601 ccgcagtatt atatatggaa tctcagcctg cgcacaaccg aggatcttca caggaacatt
      661 ttctttcagg caagcattta taatatgaat aattataaac tttacgatga aataaatgtc
      721 cctaccgcca gtttttatcc gaatcgggta attccgtcgt catggctttg gggcagatac
      781 tttgaattca aacttacata tctgctggac taggaggaaa aatgaaattc tttatcagcc
      841 ttattcagaa attcacggag agagattctt cagatctatt ctttcattac tcagtaatga
      901 tgattttctt tttttctgca ttctgcattc agaaaaaaag tccaccttcc agtgcagcgg
      961 ctgcaggcag tcttgtcaca aatacagtat ctctcggggc tcctaaaagc ataggacttg
     1021 atcttgtaac aaatataaaa gcatggaatc gcggatgtga actcaggaca ggaaaccttg
     1081 tagcagatgc attcgcctgg aaagcaaatg cggatatcgg ttttgtaaac gggggaaaca
     1141 taagagagga tcagaatgtg cctaccattc tgaaaggcac tgttccagat tcaacactgt
     1201 tcgctaaatt tatgatcttc aacaaccagg ttattgtttc tgaaatcaac ggttacagac
     1261 tcaagcaggc cctggaaaat tcaaacagac agctgactct gcccaggtac agttccgcca
     1321 ctgatgatat ggacagtgac ggttcgcagc acggcaactg ttacagcttt gggagcggga
     1381 gcggcagaat actgttcccc agttcaaacc tttccgtaga tgtgaatgtt tccaacaccg
     1441 caatgagcgt ttcaggatct gccgggtcaa acaatctgaa aacaaacaca gtcggtgcaa
     1501 gaatcaccag aattgtaatc aatggtctaa tgatttataa taatacctcc ggatcattta
     1561 cctcaggatg ggccgcagga acatcttcct gcactgtaag gcagacaagc tttacaaaca
     1621 gtgcggcctg caataaatat aaaattgcta cagtcgactt tcagattaac ggcggtgacg
     1681 gaaatttttc attcaatcct tccattacgg ccaatgcggc aggcccgaca gaatttcagg
     1741 gagacgttcc aatgagtgcg gcagtaatca atacaccaat aggaacggac tcaggcattg
     1801 catatgaata tgtgcagtca ttcacgtctg gtcctgtctt tccaaaaatc ggcaacagac
     1861 ttaatctatt tcattaaaat acattatatt ttattcattt ttttttcttc actttttttt
     1921 tctcttttta tatttt
//
LOCUS       DAOQGJ010000751         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41091_length_1935_cov_3.030851, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000751 DAOQGJ010000000
VERSION     DAOQGJ010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41091_length_1935_cov_3.030851"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="PK683_11820"
     CDS             complement(<1..1508)
                     /locus_tag="PK683_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09179.1"
                     /translation="MKSDAYWMIFWQIKNLVYGEVLNTDIRLFSGNPDKLIKKATEKI
                     RKNNDFFIYNTDVPEEYRMKILSVKTFPSDWGKNRKYRFPDAEFIDTVIIYGKKPSNH
                     GFICHIPWFNMEFTYFDTEEPDFAAASQISEMLMHSDYDTFYNYTMLSAPELRKFSIK
                     AEKEKKKRSSSVFVPEIINADLFPSQQVNFRKSMMPRSAWERQGLIRTSAEKLLKERA
                     NIIFTGESGTGKSIIIENIARLIAEERSAKTSSHSNLENLFWVTNPYRMVSGAKYFGD
                     WQEICEKMMEDLEYYNGILYIQDIIGLLQGSEGSAHSSIASYLIFFLKRGKIRIIGEM
                     SPGELERAYRLLPGFTGLFQIIPVDEMNDSSVMYVMNNYVNYIKKYTNVSFDRDSLNL
                     SFRLLKKYILGEKFPGKAVRFLKTAAVKASKLKIPEIKPDFINRVFSEKTGLPEIMFQ
                     DSLPFSENQVQNYFSKRVIGQKEAGAVFASLISTYKAGLNNPGKPIGTVLLAG"
     gene            complement(1505..>1935)
                     /locus_tag="PK683_11825"
     CDS             complement(1505..>1935)
                     /locus_tag="PK683_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09180.1"
                     /translation="AALSLYLIEELNESSDFLKIMKENYEKLLNEISEYEIITDRTSE
                     LHFRISGFGIGKILQNETGFHLHFRSGSPPSACFVLLSSINEQQSDIHKEMQKASSVI
                     RIYHSESHTADFRTGLAFNDGLTESNWKIMLQDILETDAQ"
BASE COUNT          522 a          400 c          374 g          639 t
ORIGIN      
        1 cctgccagaa gaactgttcc tataggtttt ccagggttat tcaatcctgc cttatatgtg
       61 ctgatcagag aggcaaaaac agcacctgct tccttctgtc ctatgactct cttgctgaaa
      121 taattctgca cctgattctc actaaaaggc agactgtcct gaaacataat ttccggaaga
      181 cctgtttttt ctgaaaagac tctgttgatg aaatcaggct ttatttccgg tattttcagc
      241 ttggaggcct tgactgcagc cgttttcagg aatcgtactg ccttccctgg aaatttctca
      301 ccaagaatgt attttttcag aaggcggaag cttaaattca gagaatccct gtcaaaggat
      361 acatttgtat attttttaat gtagtttaca taattattca tgacatacat aacggaagag
      421 tcattcattt catctacagg gatgatttga aacaggccgg taaatccggg cagaagccga
      481 tacgctcttt ccagttctcc aggggacatt tctccgatga ttctgatttt tccccgcttc
      541 aaaaaaaaga tcagatagga tgcgattgaa ctgtgtgctg agccttctga cccctgcaga
      601 agtccgatga tatcctgtat atacagtatc ccgttatagt attccagatc ttccatcatt
      661 ttctcgcata tttcctgcca gtccccgaaa tactttgcac cggaaaccat cctgtaagga
      721 tttgtgaccc agaaaagatt ttccaaattg gaatgtgaag aggtttttgc ggatctttct
      781 tctgcaatca gtctggcaat gttttcaatg atgattgact tgcctgtacc tgactcgcct
      841 gtaaaaataa tgttagctct ttccttaaga agtttttccg ctgaagttct gatcagtccc
      901 tgtctctccc aggcagatct gggcatcatg ctttttcgaa agtttacctg ctgagaagga
      961 aaaagatccg catttataat ttcaggaaca aatactgaag atgatctctt ttttttctcc
     1021 ttttcagcct ttatgctgaa ttttctcagt tcaggtgctg aaagcattgt gtaattataa
     1081 aatgtatcat agtctgaatg catcagcatt tcggaaatct gggacgctgc tgcgaagtcc
     1141 ggttcttcag tatcaaaata ggtaaactcc atattgaacc aagggatatg gcagataaaa
     1201 ccgtggtttg acggtttttt tccgtagata atgactgtgt ctataaattc ggcatccggg
     1261 aaacggtatt tcctgttttt tccccagtcg gaaggaaagg ttttcacaga aagaatcttc
     1321 attctgtatt cttccggaac atcagtgtta taaatgaaaa aatcattatt ttttctgatt
     1381 ttttcagttg cttttttgat cagtttatcg ggatttcctg aaaacagtct gatgtccgta
     1441 ttcagtactt ctccataaac aagatttttt atctgccaga agatcatcca gtaagcatct
     1501 gatttcattg tgcgtccgtc tccaaaatgt cctgcagcat aattttccag ttggattctg
     1561 tcagtccgtc gttgaatgcc agtccagtcc tgaagtctgc tgtatgcgat tcactgtggt
     1621 aaatcctgat tacagatgaa gctttctgca tttccttatg aatatccgac tgctgttcat
     1681 tgatgctgct cagcagcacg aaacaggccg aaggagggga gccgctcctg aaatgaagat
     1741 gaaagcctgt ttcattctga agaatttttc cgataccgaa tcctgaaatc cgaaaatgaa
     1801 gttcagatgt tctgtctgta atgatttcat attcagaaat ttcatttaaa agcttttcat
     1861 aattttcttt cattattttt aaaaaatcgg aagactcatt cagctcttct atcagataca
     1921 gagaaagagc ggctg
//
LOCUS       DAOQGJ010000752         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41152_length_1933_cov_3.627263, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000752 DAOQGJ010000000
VERSION     DAOQGJ010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41152_length_1933_cov_3.627263"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="PK683_11830"
     CDS             complement(<1..151)
                     /locus_tag="PK683_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002082105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNH09181.1"
                     /translation="MKSIERLDFKFIYNLIPEQSRVMDLGCGDGRLLALLKEKKIKGQ
                     GLEKNE"
     gene            complement(138..1244)
                     /locus_tag="PK683_11835"
     CDS             complement(138..1244)
                     /locus_tag="PK683_11835"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNH09182.1"
                     /translation="MSQEKKSVGITETFIQSVGELVLENGSVISPLEIAYETYGQLNS
                     DKSNAILVCHALSGDAHAAGYHKDSVKPGWWEDYVGPGKAFDTDKYYIISTNVIGGCK
                     GSSGPLSINPKTGKPYSSSFPFVSISDMVNAQKKLIEFLGIEKLYCVAGGSMGGMQVL
                     QWAVGYPGMMKKAICLASAAEHSAQQIAFNEVGRQAIMYDPNWNEGNFEKAPKKGLAL
                     ARMVGHITYLSDEVMRDKFGRKPPKGNIQSTDFAVGSYLIYQGESFVDRFDANSYIYV
                     TKALDHFSLGRGRELAQNLASVKCEFLVIAYNSDWLYPTYQSREIVKALEVNAIPVTF
                     CEIDFPKGHDSFLIYNKEQHHLIYHFLGKENEVY"
     gene            complement(1289..>1933)
                     /locus_tag="PK683_11840"
     CDS             complement(1289..>1933)
                     /locus_tag="PK683_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNH09183.1"
                     /translation="IIDSVKFNYGNGKFPNFTEPDPSYHGLKFWEVFGKFEPFGGVNI
                     AYIIKARVQGLRDIGACISPFNAWQILQGIETLPLRMERHSSNALKIAEHLSKHPKVS
                     WVNYPGLSSDKNHGLAKKYFKKGLYGAIVGFGIKGGVPEAKKFIDKLELFSLLANVGD
                     AKSLVIHPASTTHSQLTEAEQKSTGVTPDFIRLSVGLEHIDDLITDLDEALKAI"
BASE COUNT          510 a          461 c          391 g          571 t
ORIGIN      
        1 cttcgttttt ttccagtccc tgccctttga tttttttttc tttcagaaga gcaagcagcc
       61 tgccgtcacc gcagccgaga tccatcactc ggctctgctc cgggatcaga ttgtaaataa
      121 atttgaagtc aagtctttca atagacttca ttttcctttc ctagaaaatg ataaatcaga
      181 tgatgctgtt ccttgttgta gatcagaaat gaatcatgtc ctttcggaaa atctatctcg
      241 cagaacgtca caggtatggc attgacctcg agagctttga ctatttctct cgactgatat
      301 gtaggataaa gccagtcgct gttgtatgca atcaccagaa attcacattt cacggaggcc
      361 agattttgtg caagctctct tcctcttcca aggctgaaat gatcgagtgc cttagtgaca
      421 tagatatagg aatttgcatc aaatcgatct acaaaacttt cgccctgata gatcagatag
      481 ctccccacag caaaatctgt agactgaata ttgcctttag gaggtttcct tccaaatttg
      541 tctctcatca cctcatctga aagataagtg atatgaccta ccattctcgc cagtgcaaga
      601 cctttcttgg gagccttttc aaaattgccc tcattccagt tcggatcgta cataattgcc
      661 tgacgtccga cctcgttgaa tgctatctgc tgagcagaat gttctgcggc tgaagcaaga
      721 cagatcgcct ttttcatcat tccaggataa ccgactgccc attgaagaac ctgcattcct
      781 cccatggaac cgcccgcaac acagtacagt ttttctattc ccagaaattc aatgagcttc
      841 ttctgagcat tcaccatatc ggaaatggaa acaaaaggga aactcgaaga atacggcttg
      901 cctgtctttg gatttatgga aagaggtcct gaacttccct tgcatccgcc gatcacattg
      961 gtggaaataa tataatattt atccgtatca aatgcctttc cgggtccgac atagtcttcc
     1021 caccatccgg gctttacgct gtccttatga tatccggcag catgagcgtc tcccgaaaga
     1081 gcatggcata caaggattgc attcgatttg tcgctgttca gctgtccgta tgtttcatac
     1141 gcaatttcca gaggagaaat cactgaaccg ttctcaagca caagttcacc caccgactgg
     1201 atgaatgttt cagtgattcc gactgatttt ttttcctgac tcatgattgc agtaaaacgc
     1261 ggaacagcaa gttccgcatt catttttttc atatcgcttt cagtgcctca tcaaggtcag
     1321 tgatgagatc gtcgatatgt tcgagaccta ctgagagacg gatgaaatca ggagtcacac
     1381 cggtagactt ctgttctgcc tctgtcagct gagaatgagt agtggatgca gggtgaatta
     1441 cgagagactt tgcatcgccg acatttgcaa gaagagagaa aagttcgagc ttgtcgatga
     1501 atttctttgc ctcaggaact ccgcccttga ttccgaatcc tacaattgca ccgtacagcc
     1561 ctttcttgaa atatttcttt gccagaccgt gatttttgtc tgaactcagt ccgggatagt
     1621 tgacccagga gactttagga tgttttgaaa gatgttctgc aatcttcagt gcattggatg
     1681 aatgacgttc cattctgaga ggaagggtct caatcccctg aagaatctgc catgcattga
     1741 aaggagaaat acatgctcca atgtctctca gtccctgaac tctcgccttg atgatatagg
     1801 caatgttcac tccgccgaac ggctcaaatt ttccgaatac ttcccagaat ttcagtccat
     1861 gatagctcgg atcaggctca gtgaaattcg gaaactttcc gtttccataa ttaaatttta
     1921 ctgaatcaat aat
//
LOCUS       DAOQGJ010000753         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41161_length_1933_cov_3.200213, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000753 DAOQGJ010000000
VERSION     DAOQGJ010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41161_length_1933_cov_3.200213"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK683_11845"
     CDS             complement(<1..182)
                     /locus_tag="PK683_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09184.1"
                     /translation="MINIPEYPVHSILASLKKKLSDRKDCIVVSPPGSGKSTILPLEL
                     MNEKWTENKKIYLLEP"
     gene            complement(175..1050)
                     /locus_tag="PK683_11850"
     CDS             complement(175..1050)
                     /locus_tag="PK683_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH09185.1"
                     /translation="MRWFFRKLYSYFSFKRYSVLSTLGFRRKSVILHGLDLSVYIREG
                     KGPVLVLVHGFLDASFGFRKLIPHLPEDRKIVLIDVPSFGRSRLPEVRYLYRMDVYAG
                     LIYEIFQKLDLRDFILSGHSMGGLLSQHLAILDRKKDRRIRSLILISPGSERHNERDE
                     VRKILFPKTRVDLNILLSNLYEKDTPEVSAFVSMILLSEWGRKEYEWLAENTIAEEKD
                     IFLGVQARKIRLPVLIISAKNDRITPPASLKKLHRWIPGSRLVFLPKGKHAIHLEFPE
                     QVAEIMIKYSLNQND"
     gene            complement(1047..1268)
                     /locus_tag="PK683_11855"
     CDS             complement(1047..1268)
                     /locus_tag="PK683_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNH09186.1"
                     /translation="MKISVKVKPGSKKSSVEVVSETELSVKIHAPAQEGKANEELILV
                     LSEFYNIPKSRITLLRGLKSRTKILELDI"
     gene            complement(1272..>1933)
                     /locus_tag="PK683_11860"
     CDS             complement(1272..>1933)
                     /locus_tag="PK683_11860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNH09187.1"
                     /translation="DELKEGLPESAATGKDRDEYDYYCHHLIVLDREKDAVVGTYRIL
                     RRETAAANIGFYSETEFNMKKIYELEDELAEVGRSCVHPDYRDGSVISLLWAGLAEYM
                     RNYNLRYLFGCGSVHSLDPAAASEAFAYLKKHNALADDRFGITPLDTHILDGFDPDYK
                     ILDEKAVSRRIPVIIKGYMRLGAKICGTPAVDRVFGTTDMFVLFDFEQISAKYGKKFV
                     S"
BASE COUNT          525 a          430 c          391 g          587 t
ORIGIN      
        1 ctgggctcga gaagataaat tttcttattt tctgtccatt tttcattcat cagttcaagc
       61 ggaagaattg tgctttttcc tgaaccggga ggagaaacga ctatgcagtc ctttctgtct
      121 gaaagttttt ttttcagact ggcgagtatg gaatgaacag gatattccgg gatattaatc
      181 attctgattt aatgaatatt taatcatgat ctcggcaacc tgctccggaa attcgagatg
      241 aattgcatgt tttccctttg gaagaaacac gagcctgctt ccgggaatcc atctgtgcag
      301 tttcttaagt gaggccggag gagtaatcct gtcatttttg gcagagatga taaggacagg
      361 aagtctgatt tttctggcct gaactccaag gaagatatcc ttctcttctg caattgtatt
      421 ttctgccagc cattcatact ccttccttcc ccattctgaa agaagaatca tcgaaacaaa
      481 tgcggaaacc tcaggagtgt ccttttcgta aagattggag aggagaatgt tcagatcgac
      541 tcttgtctta ggaaaaagaa tctttctgac ctcatctctt tcattgtgcc tttccgatcc
      601 tggagaaatc agaatcagac tcctgattct tctgtccttt ttcctgtcca gaattgcaag
      661 atgctgtgaa agaagtcctc ccatggagtg accggaaaga atgaaatcgc ggagatccag
      721 cttttgaaat atttcgtata tgagtcctgc atacacatcc attctgtaca gatatctgac
      781 ctccggaagt ctgcttctgc cgaatgaggg aacatcaatc agtacgatct tcctgtcttc
      841 cggaagatga gggatgagtt ttctgaaacc gaaggatgca tccagaaaac cgtgtacaag
      901 cacaagaaca ggcccttttc cttcgcggat gtagacggaa agatccagtc catggagaat
      961 gactgatttt cgccggaatc ctaatgtgct taaaacggag tatcttttaa agctgaagta
     1021 actgtacagt tttctgaaaa accatctcat atatccaatt ccagaatctt agtcctggat
     1081 ttcaagcctc tcaggagagt aattctggat tttggaatat tgtagaattc cgaaagaaca
     1141 aggatcagtt cttcgtttgc ctttccttcc tgtgcaggag catggatttt cacagaaagt
     1201 tcagtttcag aaacaacctc aactgaactc ttctttgatc caggctttac cttcacggaa
     1261 attttcattt ttcaggacac gaactttttt ccatattttg cactgatctg ttcaaagtca
     1321 aacagtacaa acatatccgt tgttccgaag accctgtcca ctgcaggagt tccgcagatt
     1381 tttgctccga gtctcatata tcctttgatg atgactggaa ttcttctgga aacagccttt
     1441 tcatccagta ttttataatc aggatcaaaa ccatccagaa tatgagtatc cagaggcgtt
     1501 atcccgaacc ggtcgtcagc aagagcattg tgctttttca gataggcaaa cgcttctgag
     1561 gcagctgccg gatccagaga atgaactgaa ccgcatccga aaagataacg aagattataa
     1621 tttctcatat attcagcaag tcctgcccag agaagagaaa tgactgatcc gtcccggtaa
     1681 tcaggatgaa cgcagctcct gcctacttct gccagctcat cttccagctc gtagattttt
     1741 ttcatattga attctgtttc agaatagaag ccgatatttg cggccgcagt ttctcttctg
     1801 agaatcctgt aagtcccgac tactgcatcc ttttcgcggt caagaacaat cagatgatga
     1861 cagtaataat cgtattcatc tctgtccttt cctgttgcag ccgattcagg cagtccttct
     1921 ttcagttcat cat
//
LOCUS       DAOQGJ010000754         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41165_length_1933_cov_2.953142, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000754 DAOQGJ010000000
VERSION     DAOQGJ010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41165_length_1933_cov_2.953142"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /gene="rhuM"
                     /locus_tag="PK683_11865"
     CDS             <1..710
                     /gene="rhuM"
                     /locus_tag="PK683_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HNH09188.1"
                     /translation="PQGTKFRIWATQRLKEYIIKGFTLNDDRFKSGNSMNYFTELQER
                     IREIRLSERFFYQKIKDIYTTSIDYDPKDESTILFFKTVQNKLLWAISEQTAAELVYR
                     RSDASLPLMGMLSYDKKSAGSIKKSDVSIAKNYLNEDEMKLLGLLVEQYLAFAETMAQ
                     QRTPMYMKDWISRLDTILQLNGRELLNHAGKISHESALQKSEAEYEKYQISRREKEKE
                     ESLKELENDIDKLRKKK"
     gene            915..1211
                     /locus_tag="PK683_11870"
     CDS             915..1211
                     /locus_tag="PK683_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNH09189.1"
                     /translation="MPYTYVLKCNDGTYCTGSSWDLNKRLAEHNAGQGGAYTAKRLPV
                     KLVYFEEFDRIEDAFCREKEIQNLDDNGIRTLVKENYETLKESAREIFGAGFFF"
     gene            complement(1219..>1933)
                     /locus_tag="PK683_11875"
     CDS             complement(1219..>1933)
                     /locus_tag="PK683_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH09190.1"
                     /translation="TGSVSELAKSASANAESGMNEIRNSRKKMDILLRAINDTEENLS
                     SLKGRTSEIASILVVIQGISKQTSLLALNAAIEASRAGDAGKGFAVVADEIGKLSEST
                     RKSVEEIKTIIGNLQVTVAGTETAVSSETKLVKESVFSLAKSDSFLQEISSVFQEVFS
                     DVEVLFSSQKEISMKSEKLISKTKFTEQTAEETKISTESTVSSLLSQTETMQRLNEQA
                     EELARLVKKLRELTERFIT"
BASE COUNT          573 a          392 c          414 g          554 t
ORIGIN      
        1 cgccacaggg cactaaattt cgaatctggg ccacccagag gcttaaagaa tacatcatca
       61 aagggtttac cctcaacgat gaccgattca agtctggaaa ttcaatgaat tactttaccg
      121 agcttcagga aagaattcgc gaaattcgtc tttcggagag gtttttctac cagaaaataa
      181 aggacatcta taccacaagc atcgactatg atccgaagga tgaaagcacg attctttttt
      241 tcaagactgt gcagaataaa cttctttggg caatcagtga gcagacagcc gctgaacttg
      301 tgtatagaag atcggacgcg agtctccctc tcatgggaat gctgtcctat gataaaaaat
      361 ctgcaggttc cataaaaaag tcggatgtaa gcattgcaaa gaactatctg aatgaagatg
      421 aaatgaaact gcttggcctg ctcgtggagc agtatctggc atttgcggaa acgatggcac
      481 agcagaggac accgatgtat atgaaggact ggatttcgag actggatacg attctgcagc
      541 tgaacggaag agagcttttg aatcatgccg gaaaaatcag ccacgaatcg gctcttcaaa
      601 aatcagaagc ggaatatgaa aagtatcaga tttccagaag agagaaagaa aaagaagaaa
      661 gtctgaaaga gctggaaaat gatattgata aactgaggaa aaagaaatga aatatgtatc
      721 caatataaac tgtagcaaac ccccattccc tgtgtccccc gtggtaaaaa aaataaacca
      781 cggggaagag gggaatatgg ggaaggtaaa agatatgcat tcctataaac tgtagcaaac
      841 ccccatatcc ccgtggtaaa aaactccgtt ttttactcga ctcattcaaa tctgtttttt
      901 aaactgctcc gcatatgcct tatacatatg tcttaaaatg caatgacgga acatactgca
      961 caggaagttc ctgggatctg aataaaaggc tggcagagca caatgcaggg cagggcggtg
     1021 cttatactgc aaaacgtctt cctgtaaagc tcgtctattt tgaagagttt gacagaattg
     1081 aagatgcatt ctgcagagag aaagagattc agaatctgga tgacaatgga atcagaactt
     1141 tggtaaaaga aaattatgaa actctgaaag agtctgccag agaaattttc ggagcaggtt
     1201 ttttcttctg aatcatgatt atgtaatgaa tctttcagtg agttctctca gttttttcac
     1261 aaggcgtgcg agttcttctg cctgttcatt gagtctctgc attgtttctg tctgagaaag
     1321 aagagagctg actgtgctct ctgtgctgat tttggtctct tctgcagtct gttctgtgaa
     1381 ctttgtcttg ctgatcagtt tttcactttt cattgagatc tccttctgag aagaaaaaag
     1441 gacttccaca tccgaaaaca cttcctgaaa cactgacgat atctcctgca gaaaactgtc
     1501 tgattttgcc agtgagaaaa cactttcttt tacgagtttt gtttcgctcg aaactgctgt
     1561 ttctgtacct gccactgtga cctgaagatt gcctatgatt gtcttgattt cttccacgga
     1621 ttttcttgtg ctctccgaaa gttttcctat ctcgtctgca actactgcaa acccttttcc
     1681 ggcatcaccg gctctcgacg cctctattgc cgcattgagt gcaagcaggc ttgtctgctt
     1741 ggatattccc tgaatgacta caagaatgct tgcaatctcg gatgttcttc ctttcagtga
     1801 agaaagattt tcttccgtat cattgattgc tctgagaaga atgtccattt ttttccggga
     1861 atttctgatt tcattcattc ctgattcagc atttgcagat gctgattttg caagctcaga
     1921 aacagatcct gtt
//
LOCUS       DAOQGJ010000755         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41231_length_1931_cov_3.710554, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000755 DAOQGJ010000000
VERSION     DAOQGJ010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41231_length_1931_cov_3.710554"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1931)
                     /locus_tag="PK683_11880"
     CDS             complement(<1..>1931)
                     /locus_tag="PK683_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09191.1"
                     /translation="DPPPIGTGGPIIDTSGTVTGTVIKTTDVVDRYQDIVIEFSESMD
                     RATVCNGETGGALTLKINGGAVIPASDMSCRWSTDRQLLIRHYKSFLPYTKYDIIISN
                     AAKPSYDSASRSFTNFTNAADAVNNVGGNTFSFRTESAFNPTSVTLNFNGTNYNYDGT
                     KGMNLDSYGILSPANEVKLSYSTADFSRAEIITLKKVGTDPSIGYVICNTGCVNNLSL
                     LNLNTALPAALQTSNGMNAYYLELQVRVTTVPLQYKRYYRTVNFNWGNHLQVTTSSPA
                     PVLPALPVSYGPEQKIPMVGMLVLDKGPSGGSSEGTIDVLGHFIELYAGKFFTLNSDY
                     ASPGTPVNESLNQMLRRNASTDLPYAQCTDVNSLTKSACMDWSHYGDKNTFTQVNPYG
                     LFTVLEGYAKRLDNGLPYSFPVGGPYPTAKSPFPYLTQIGPFKGLSGVDRTYPIPNLT
                     PLLSSTISAGVDQIITNALNGIIPQIVLDFMSDFISQLIGAVLGGILGGIVAAVNGIA
                     PLQLLYDFNADAYVRQAVFGKKNPPAFSSGAPWYNYAPDTSQEADLLRAVIDVVPNSP
                     SGPTPKLEIKVKAKEINAELMANLKVTNVDLQFYFIKLIPFDPIYVNISSDPLDWFNE
                     LCPAGVCTGLPDLLSPKNW"
BASE COUNT          544 a          415 c          442 g          530 t
ORIGIN      
        1 ccagttcttg ggactgagca gatcaggaag ccctgtgcat actcctgcag gacacaattc
       61 attgaaccag tcaagcgggt ctgaactgat atttacataa atggggtcaa acggaatcag
      121 cttaatgaaa taaaattgga gatctacatt tgtcaccttg agattagcca tcagttctgc
      181 attaatctcc tttgccttga ctttaatctc cagctttgga gtgggaccgc ttggactgtt
      241 tggaacaaca tctatgactg ctctcagaag atccgcttcc tggcttgtat ccggcgcgta
      301 attataccaa ggtgcaccgg aagaaaaagc agggggattc tttttcccaa aaacagcctg
      361 tctgacatag gcatccgcgt taaaatcata taaaagctga agcggagcga ttccgttgac
      421 ggcagcaaca attcccccaa gtatgcctcc aagaactgcg cctataagct gggatataaa
      481 atcagacata aaatccagaa caatttgagg aataattcca ttgagtgcat ttgtaatgat
      541 ctgatcaaca cccgcactga ttgttgaaga cagaagaggt gttagattgg gaattggata
      601 cgttctgtct actccagaaa gtcctttgaa aggtcctatc tgggtcagat aaggaaaagg
      661 agattttgca gttggatatg ggccaccgac aggaaaactg taagggagtc cattatccag
      721 acgcttagca tatccttcca atacagtaaa aagtccatat ggattgacct gagtaaaagt
      781 atttttatct ccataatgac tccagtccat acaggctgat ttagtaagac tgtttacatc
      841 cgtgcattga gcatatggca gatctgtgga ggcatttcga cggagcatct gattcagact
      901 ctcgttgact ggagttcccg gactggcata gtcgctgttc agagtaaaaa actttccggc
      961 atacagctca ataaaatgtc ccagcacatc tatcgtgccc tctgaagagc ctccagaagg
     1021 ccctttgtcc agcacaagca ttcccaccat agggattttc tgctcaggtc cgtaggaaac
     1081 aggcaaagca ggaagaaccg gtgcaggtga agaagttgtc acctgaagat gattgcccca
     1141 gttgaaattg acagttctgt agtacctttt atactgcagt ggaactgttg tcactctgac
     1201 ctgaagttct aaataataag cattcatacc attagaagtc tgcagtgcgg caggcagtgc
     1261 tgtgttcaga ttcaaaagtg acaggttgtt gacgcagcct gtattgcata tcacatatcc
     1321 tatggaagga tctgttccta ctttcttgag tgttatgatt tctgcacggc tgaaatctgc
     1381 agtagagtag gataatttga cttcattggc aggagaaaga attccgtaag aatccaagtt
     1441 catacccttg gtaccgtcgt aattataatt tgttccgttg aagttcagag tgacagaagt
     1501 tggattaaat gctgattcag tccgaaagct gaatgtattt ccgccgacat tgttgactgc
     1561 atccgccgca ttcgtgaagt ttgtaaaaga tcgggaagca ctgtcataag acggctttgc
     1621 agcattactt ataatgatat cgtattttgt gtagggaagg aaacttttgt aatgtcggat
     1681 aagaagctgt ctgtcagttg accatcggca gctcatatcc gatgcaggaa tcactgctcc
     1741 tccgttgatt ttcagggtca gcgcaccgcc cgtctcaccg ttgcagactg tagcacggtc
     1801 catactttct gaaaattcaa tgactatatc ctgatacctg tctacaacat ccgtagtctt
     1861 aattacagtg cctgtcacag taccggaagt atcaataatc ggaccgccgg ttccgatagg
     1921 aggtggatct g
//
LOCUS       DAOQGJ010000756         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41242_length_1931_cov_3.198827, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000756 DAOQGJ010000000
VERSION     DAOQGJ010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41242_length_1931_cov_3.198827"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="PK683_11885"
     CDS             <1..1783
                     /locus_tag="PK683_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNH09192.1"
                     /translation="IEVNRAFYSMADFTGTSVIGNTASEIYGLSSDVIAEFWKEHRGS
                     NTTQYTKFISPLNGRHFMISTSPFLNDRFVTVFFDITDSEEARTKLQESENRFRTMAD
                     CSPALIWMSGTDSLCSFFNKTWLEYTGKSMEEELGCGWAKGVHPDDLENCIKIYTECF
                     QNRKPFAMEYRLKRKDGTYGWIYDQGVPRFSDDSEFEGYIGSCTDISELKKANEEIIR
                     ISNEREMILESTGSGVLLLKDRKIVWANKRAANLFGYTLEELTSISLIKPYPYAEIFN
                     HLGSEMYSVLARAFRFSTETEQKKKSGETFWCRFTGKLLDPKDPEKGSIWILEDITQR
                     KQAEFSLNICHKSLEIMQSQLAEKNRELGEINEVKEKFLSVIANKLSSPFRTFLGLSE
                     LMADESDSLSKKDMQEFSREIHLLAEKLHKNVDNLLSWSAVRNGTVQFSPILCNLRKI
                     AQNSISLLSESARQKGIDFFCSVKEDIYMLADHSMIEAVLRNLISNAVKFTPKGGRIS
                     VSTSEYEDEILISIEDSGIGMDEKILKSLFISNEKISSPGTEEESSTGLGLLLCQEFI
                     RRHNGKIRAESVPGKGTSFYVSLPKHL"
BASE COUNT          611 a          375 c          445 g          500 t
ORIGIN      
        1 catagaagtc aacagggcct tttattccat ggcagacttt acaggaactt cggtgattgg
       61 aaatactgct tcagagattt acggactgtc aagtgatgtc attgctgaat tctggaaaga
      121 acatcggggg tcgaacacga ctcagtacac caagttcatc agtcctttaa acggaagaca
      181 ttttatgata tccacatcgc ctttcctgaa tgaccgattc gtcaccgttt tctttgatat
      241 tacagattca gaggaagcac ggacaaaact gcaggaaagc gaaaacagat tccgtactat
      301 ggcggactgc agtcctgcac tgatatggat gtcaggaaca gactcactgt gcagtttttt
      361 caacaaaacc tggcttgagt atacaggaaa atcaatggaa gaagagctcg gctgcggctg
      421 ggcaaaaggt gttcaccctg atgatcttga aaattgcatt aaaatatata ccgaatgctt
      481 tcagaacaga aaaccgtttg caatggaata cagactgaag cggaaggatg gaacttacgg
      541 atggatttac gatcagggcg ttcccagatt ttcagatgac agcgagtttg aaggatatat
      601 cggatcgtgc acagacatat ctgaactgaa gaaagcgaac gaagaaatta ttcggatatc
      661 caatgagaga gaaatgattc tggaaagcac aggaagcggt gttcttctgc tgaaggacag
      721 gaaaattgta tgggccaata aaagagcggc aaatctgttc ggatatactc tggaagaact
      781 gacaagcatc agtctcatca aaccttatcc atacgcagag atattcaatc atctgggttc
      841 tgagatgtat tccgttcttg caagagcatt tcgattttcc actgagaccg agcagaaaaa
      901 aaaatcagga gaaactttct ggtgcaggtt tacaggaaaa ctgctcgatc ctaaagaccc
      961 ggaaaagggc tccatctgga ttctggaaga cattacccag agaaaacagg cggaattttc
     1021 attaaacatc tgtcataaat cattagaaat aatgcagtct cagctcgctg aaaaaaacag
     1081 agaacttggg gaaatcaatg aggtgaagga aaagtttctt tcggttattg caaataaact
     1141 gagttctccc ttccgtacat ttctgggact gagtgagctg atggccgatg aatcagattc
     1201 attatcaaaa aaagatatgc aggaattcag cagggaaatt catcttctcg ctgagaagct
     1261 gcacaagaac gtggacaatc ttctgagctg gtctgcagtc aggaatggaa ctgttcagtt
     1321 ctctccgatt ttatgcaatc tcagaaaaat tgcacagaac agcatttctc tgttgagcga
     1381 atctgccaga cagaagggta tagatttctt ctgctcagtc aaggaggaca tttatatgct
     1441 tgctgatcat tccatgattg aagcagtgct cagaaatctg atttcgaatg ctgtcaagtt
     1501 tactcctaaa ggcggaagaa tttccgtttc tacttccgag tatgaagatg agattctgat
     1561 ttctattgaa gactccggaa tagggatgga tgagaagatt ttgaaaagtc ttttcatttc
     1621 gaacgaaaaa atctcaagtc ccggaacaga agaggaatca agtacagggc tcggacttct
     1681 tctatgtcag gaattcattc gaaggcacaa tggaaaaatc agagccgaaa gcgttccggg
     1741 aaaaggaacc tcattctatg tatctctgcc gaaacattta tgagtaaaca actcacctta
     1801 cacaggcggg gcgttttgac agagagcccg gcggtttcac aaacgtggaa gttgccgttt
     1861 ttactgattc atcgcaagct gtcaaactgc tggattttac taatgctttc tgagaattac
     1921 agcatctgga c
//
LOCUS       DAOQGJ010000757         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41390_length_1927_cov_3.170406, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000757 DAOQGJ010000000
VERSION     DAOQGJ010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41390_length_1927_cov_3.170406"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="PK683_11890"
     CDS             complement(<1..1497)
                     /locus_tag="PK683_11890"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNH09193.1"
                     /translation="MSDFFYSDPFPLKDDGTEYKLLTKDYVSTVPFGDKEILKVEKEA
                     LTLLTQEAIKDVSFFLRTRHLEKVRKILDDPEASDNDRFVATALLKNAVIAAEMQLPS
                     CQDTGTAIVTAKKGEYVITGANDAEAISLGIFNTYKERNLRYSQVVPYTMYEEKNSGS
                     NLPAQVDIYSSYGNKYEFLFLAKGGGSANKTYLFQETKALLNPASLEKFIIEKVNSLG
                     TAACPPYHIAVVIGGTSAEMTLKTVKLASTGYLDTLPTSGGKEGFAFRDIELEEKMLE
                     ASRKSGIGAQFGGKYLAHDFRVIRLPRHGASCPVGLGVSCSADRNIKGKITKDGVFLE
                     KLEYNPARFLPKMEQIQMTADTVHINLNQPMEEIIKILTKYPVKTRVMLSGKLIVARD
                     IAHAKLKEKLDKGEALPDYFKNHPIYYAGPAKTPEGLPSGSFGPTTAGRMDSYVPLFQ
                     EKGYSLVTLAKGNRSKVVTDSCKKHGGFYLGSIGGPAALLAKENIKKVE"
BASE COUNT          496 a          423 c          400 g          608 t
ORIGIN      
        1 ctccactttt tttatattct cctttgcaag aagtgcagca ggacctccga tggaacccag
       61 ataaaaacct ccgtgctttt tacagctgtc tgtgactacc tttgatctgt ttcctttcgc
      121 aagagtaaca agagagtatc ctttttcctg gaaaagaggt acataactgt ccattcttcc
      181 tgcagttgta ggaccgaatg aaccggaagg aagtccttca ggagtctttg caggacctgc
      241 gtaatagatc ggatgatttt tgaaatagtc aggaagtgct tcacctttgt cgagcttctc
      301 cttcagcttt gcatgtgcaa tgtctcttgc cacaatcagt tttccggaaa gcatcaccct
      361 tgtcttcacc ggatattttg taaggatttt tataatttct tccatcggct gattcagatt
      421 gatatgcaca gtgtctgcag tcatctgtat ctgctccatc ttaggcagga atctggcagg
      481 attgtattcc agtttttcca gaaagactcc atccttagta atctttccct tgatgtttct
      541 gtcggcactg cagctgactc cgagtcctac cggacaggag gctccgtgtc tgggaagtct
      601 gatcactctg aaatcatgtg caaggtattt tcctccgaac tgtgccccaa ttcctgattt
      661 tcgtgaggct tcgagcatct tttcttccag ctcaatatcc ctgaatgcaa atccttcctt
      721 tccgcctgat gtgggaagag tatccagata tcctgtggat gcaagcttca ctgttttcag
      781 tgtcatctct gcagaggttc ccccgatcac aaccgcaata tggtagggag gacaggctgc
      841 tgttcccaga gagtttactt tttctatgat gaatttttcg agagaggccg ggtttagcag
      901 tgcctttgtt tcctgaaaga gataagtctt gttcgcagac cctccccctt tggccagaaa
      961 gagaaactcg tatttatttc cgtagctgga atagatgtcc acctgtgcag gcagattgga
     1021 gcctgaattt ttttcctcgt acatggtata aggcacaacc tgagaataac gaagatttct
     1081 ttccttgtat gtattgaaaa ttccgagaga tatggcctct gcatcattcg cacctgtaat
     1141 cacatattcc ccttttttgg cagtgacaat tgcagtgcct gtatcctgac agctgggaag
     1201 ctgcatttca gctgcaataa ccgcattttt cagaagtgct gtggcgacaa acctgtcatt
     1261 gtccgaggct tccgggtcat ccaggatctt tcttactttt tccagatgtc ttgtcctgag
     1321 gaaaaaagag acatctttta ttgcctcctg agtaagaagg gtcagagcct ctttttcgac
     1381 tttaagtatt tctttgtcac cgaacggaac tgtgcttaca tagtcttttg taagaagttt
     1441 atattctgtt ccgtcgtctt tcagagggaa tggatcgctg taaaaaaaat ctgacatatc
     1501 tgctcctgta tttcccagat ttctaatctc tttacattcg atcagaaatc aatatggcag
     1561 ggggcctact ggcagttaaa agaggcttgg cagccccttt taaatccccg cctttcgtta
     1621 cattgaatga tgacaaaaat tcacttttaa tcaatactgg attgatccta ttgtaatctg
     1681 ctcagaaatc tgggtattta agtaacagaa tttacagaac tctcaggaat taaagaaaaa
     1741 aaagccccta tttttcgata ccattgtgtt tgatgacagc ctgcaaggaa ataaagggat
     1801 aagggagaaa taaaaaacag taaactgaat ctaatgcctg aaataaggaa gcagatcagt
     1861 ttatttcaag tcgatacgtt tgaacggaac agctttgtca tctttgaaaa gaagactttc
     1921 attctgt
//
LOCUS       DAOQGJ010000758         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41439_length_1926_cov_2.791021, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000758 DAOQGJ010000000
VERSION     DAOQGJ010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41439_length_1926_cov_2.791021"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1021
                     /locus_tag="PK683_11895"
     CDS             2..1021
                     /locus_tag="PK683_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HNH09194.1"
                     /translation="MDEREIPDALKILCRLRSFSEIRNGIFSPIQRLRFENPNAEIYY
                     AGPESSFTRAFLERHPDCREFAGESCDTVISPEDFASWTLISNISSVILSDLELYKKQ
                     KKWTDKILMKASSYHHVGKLKHLYIHQSAVIYPGVVFDVTSGPVIIDKDVKITSFSFL
                     EGPLYIGPGTHIDNARITGGTVIGSVCKIGGEVENSIFGDFSNKHHEGFVGHSHIGNW
                     VNMGALSTTSDLKNNYGVVNVKIESSIINTGTIKFGAMIGDFSKIAIGVMLNTGASVD
                     IGCNVVSERVSGYLRPFTWADSGTRYRLDQFLTDSRKIMARRNQTLSQSEEELIRHVY
                     EAGLK"
     gene            1018..1881
                     /locus_tag="PK683_11900"
     CDS             1018..1881
                     /locus_tag="PK683_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH09195.1"
                     /translation="MSQRMQEHQRNGKFVNVDGLNIFTVDQGKGEVILLLHGLFSTSF
                     SFRKLIPILSENHRVIAPDLPGVGLSEKFQNEYSHRALADFLYKYISEMTDQRVHLVA
                     YDYGAAVSALLLNEHPEVVKTYTVISGFTYLHSVFQYRPLFFIHTRVIGSLVGLFLNE
                     KTIKWLYNKFLLGKSSPMEDELAMDYYFLLFHGRNKKNFIRMSQCIDRKVYARKDMET
                     GMQRMIGGRQILIAEEDFNTSYKEIENMKLFLRLSIAHMLPGGRMIMEDAPEECASKI
                     EALVKTFSRKV"
BASE COUNT          605 a          338 c          433 g          550 t
ORIGIN      
        1 gatcgatgaa agagaaatac cggatgcatt gaaaattctc tgcagactga gatcattttc
       61 tgaaatcaga aacgggatat tcagcccaat ccaaagactt cgttttgaga atccaaatgc
      121 agagatttat tatgcaggac ctgaatccag tttcactcgt gcatttctgg aaagacatcc
      181 tgactgcagg gaatttgcag gcgaaagctg tgatacagta atttctccgg aagactttgc
      241 gagctggact ctgatttcaa atatttccag tgtgattctt tcagacttgg aactttataa
      301 gaaacagaag aaatggacag ataaaattct gatgaaggct tccagttatc atcatgtggg
      361 taaactcaag catctttaca ttcatcagag tgctgtcatt tatccaggag ttgtatttga
      421 tgtgacaagc ggtcctgtca tcatagacaa ggatgtgaaa attacatctt tcagttttct
      481 ggaaggcccc ctgtatatcg gaccaggcac acatattgac aatgcaagaa ttacaggcgg
      541 aactgtgata ggctctgtct gcaaaatagg cggggaagtg gagaattcca tttttggaga
      601 tttcagcaat aaacaccatg aggggtttgt cggacattct catatcggga attgggtcaa
      661 tatgggtgca ctttccacaa caagtgatct gaagaataat tacggtgtag taaacgtaaa
      721 gattgaatct tccataatta atacaggaac aataaaattt ggtgcaatga ttggagactt
      781 ttccaagata gcaataggag tcatgctgaa tacaggtgct tctgtagaca taggatgcaa
      841 tgtagtttct gaaagagttt ccggctatct caggccgttt acctgggccg attccggaac
      901 aaggtacaga ttggatcagt ttctgactga ttccagaaaa attatggcca gaaggaacca
      961 gaccctgtcc cagtcggaag aagaactgat caggcatgta tatgaagccg gtttaaaatg
     1021 agtcagagaa tgcaggaaca tcagagaaac ggaaaatttg taaatgtgga tggactgaat
     1081 atttttacag tcgatcaggg aaaaggagag gtgattctgc ttcttcatgg acttttcagc
     1141 acatcttttt ctttcaggaa actgattccg attctttccg aaaatcacag agtgattgca
     1201 cctgatcttc caggagtcgg actttcggag aaatttcaga atgaatattc tcacagagcc
     1261 cttgccgatt ttctttataa atatatcagt gaaatgactg atcagagagt tcatctcgtt
     1321 gcctatgatt acggtgcggc agtatccgca cttcttctga atgaacaccc tgaggttgta
     1381 aagacctata ctgtaatttc aggctttact tatcttcaca gtgtgtttca gtacagaccg
     1441 ctttttttca ttcataccag ggtaatagga agtcttgtcg gtcttttttt gaatgaaaag
     1501 acaatcaaat ggttatataa taagtttctt ttaggaaaaa gcagtccaat ggaagatgag
     1561 cttgcaatgg attattattt tctccttttt cacggaagaa acaagaagaa tttcatcaga
     1621 atgagtcagt gcatagacag aaaggtatat gcaagaaagg atatggaaac tggaatgcag
     1681 agaatgatcg gtggacggca gattctgatt gcggaagaag attttaatac ttcctataag
     1741 gaaatcgaaa atatgaagct tttcttaagg cttagcatag cacatatgct gcctggagga
     1801 aggatgatca tggaagatgc ccctgaagaa tgtgcttcaa aaatagaagc tctagtaaaa
     1861 accttctctc gaaaagtctg atgtcttacg aaattcttgc ttcagcagtc aatacagggt
     1921 cacagg
//
LOCUS       DAOQGJ010000759         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41483_length_1925_cov_2.400535, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000759 DAOQGJ010000000
VERSION     DAOQGJ010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41483_length_1925_cov_2.400535"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK683_11905"
     CDS             complement(<1..206)
                     /locus_tag="PK683_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09196.1"
                     /translation="MKSYRFGLLLVLIFLQCTTTVKIPVMNKSPQGFMEFMRGGRDIG
                     MYVYKKDNADLPSGGEADWKTVVHG"
     gene            complement(300..1172)
                     /locus_tag="PK683_11910"
     CDS             complement(300..1172)
                     /locus_tag="PK683_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH09197.1"
                     /translation="MKIFQEKFNSFGHEIEVKSTDSGKENWMFLLHGFNDSKETFLFL
                     EDSLLSKFNLLSFDFRGHGNSDWSRDGIYHYAEHFLDIHNIARQFLPEKFFMLAHSMG
                     ASLASRYTGMYPDSVKGLICLEGFSGLQPPEREIDRIKGWLNSQVAKGQKSTAPRRPM
                     KREEALQKLSLIYSGLPKEKVELLLEPLVRKTVENKYVWKNDPSLRTSSNPIPFSPFL
                     SRALWSKILCPVLILYGKKSHLWPENLEEVLSHFKNREFHILENSGHNMHHDEPEKTL
                     EHIFEFLKKNSLVE"
     gene            1221..1643
                     /locus_tag="PK683_11915"
     CDS             1221..1643
                     /locus_tag="PK683_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HNH09198.1"
                     /translation="MTKKKTPEKKDKEKMSQQKSSSKTKSNEESSGSAKAKGGKSAPK
                     SAKESETSDNPLGKKFSCYNCGTKFYDLNKPEKICPKCKADQLSKPAQKGKSLRAKIS
                     EFDVTEEEAVAPDEEIDGEDVIEESEAEEPIEVEEEEV"
BASE COUNT          577 a          390 c          408 g          550 t
ORIGIN      
        1 ccgtgcacga ctgtcttcca gtcggcctct cctcctgagg gaagatccgc attgtctttt
       61 ttataaacgt acattccaat atctcttccg cctctcataa attccataaa gccctgcggt
      121 gatttgttca tcacaggaat tttgacagtc gtagtgcatt gaaggaaaat taagacaagg
      181 aggagaccga atcggtaaga tttcatagga acatctgatg gaaaaccgaa gatttttcaa
      241 ttctttttct gctgaaattt ttctttacgt ttcactgcct gataaaactg agcaaaagat
      301 cattctacaa gactgttctt tttcagaaat tcaaatatat gttccagtgt tttttccggc
      361 tcgtcatggt gcatattatg acctgaattt tccaggatat gaaactccct gtttttgaaa
      421 tggctgagca cttcttccag gttttccggc cagagatgtg attttttacc gtaaagtatc
      481 agcacaggac agagaatttt tgaccataat gctctggaaa gaaaagggga aaacggaatg
      541 ggattggagg atgttctaag cgacggatca tttttccaga catatttgtt ttccactgtt
      601 tttctgacga gaggttcaag cagaagttcc actttttcct ttggcagccc agagtatatc
      661 agagaaagtt tctgaagagc ttcttctctt ttcatcggcc tcctgggagc agtggatttc
      721 tgtcctttgg caacctgtga attcagccat cccttgattc tgtctatttc tctttcagga
      781 ggctgaagtc cgctgaatcc ttccagacat atcagtcctt tcactgaatc ggggtacata
      841 cccgtatacc tggaggccag gcttgctccc atggaatgtg ccagcatgaa aaatttttca
      901 ggaagaaact gtctggcaat attatgaata tccagaaaat gttctgcata atggtagatt
      961 ccgtcccggc tccagtctga atttccatga cctcggaagt cgaaactgag aagattgaac
     1021 ttggaaagga gagagtcttc caggaaaaga aatgtctcct tgctgtcatt gaatccatgc
     1081 agaagaaaca tccagttttc ctttccggaa tcagtgcttt ttacttctat ctcatggccg
     1141 aatgaattga atttttcctg aaaaattttc atatttcctc ttgacaaaaa aacgtattct
     1201 tcgaatcatt tcctattctt atgactaaga agaagactcc cgagaaaaaa gacaaagaaa
     1261 aaatgtctca gcagaaaagt tcatcaaaga caaaatcgaa tgaagaatct tccggttctg
     1321 caaaagcaaa aggagggaag tctgctccta agtctgcgaa ggaaagtgaa acttctgaca
     1381 accccctagg gaagaagttt tcctgctata actgcggaac gaaattttat gatttgaaca
     1441 agccggaaaa aatctgtcct aaatgcaagg cagatcagct ctccaaaccg gctcagaaag
     1501 gaaagtcact tcgtgcaaaa atatctgaat ttgatgtgac agaggaagag gcagttgctc
     1561 cggatgaaga aatagacgga gaggatgtaa ttgaagaatc agaagcggaa gaaccgatag
     1621 aggtggaaga ggaagaggtc tgaaaaggat tttcattctt gctgccccca caggaggggg
     1681 caagactgga atctgtgtca gactgaaccc tgattttttt gaagtcgttt ctgttgattc
     1741 cagacaggct taccagtttc tgaatattgg aacagctaaa ccggagaaat cggaacaggc
     1801 tgcagttcct caccatctga tagatttcat tcatccaaag gatgatatca atgccagaat
     1861 gtactctgaa aacattcgtt cctgtgtatt ggatattctg aaaagaaaca aaacccccat
     1921 tttaa
//
LOCUS       DAOQGJ010000760         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41553_length_1923_cov_1.827088, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000760 DAOQGJ010000000
VERSION     DAOQGJ010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41553_length_1923_cov_1.827088"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PK683_11920"
     CDS             complement(<1..1143)
                     /locus_tag="PK683_11920"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNH09199.1"
                     /translation="MRKEIFIILYCLLLSFCQKSPLEKAQKNDSVRLLFLYVSSLTSQ
                     SAVITFSCPESVTGIVGYGIGSIQKYQASLSPSKIHSISLAGLTSDQKYTYAVFCGSV
                     SPKTVFPQSFSTPNPDPLLVIKKRSIWIIGGIGVSGLPVAQVDMYDPATNTWYPSVTS
                     LPTPRAFASVTVISGKIYVIGGIKASGTSFSVSNAVEMYDLDKNAWTALSSMPNSLTG
                     ALAGKMNNEIYMIGGTTTTDMTTGTILNTVYRFQPSVGSTGTWTQYTVQGGSLLPRID
                     MAGCGNDGTIYMTGGRLYSSGAEQSSSDAFIPGANTTTSLIEASLNGARHGSASSCYR
                     PLPSDPYPADSPALLVFGGSTATNVFIPASSITPSALYDYYPVGGTT"
     gene            complement(1140..1340)
                     /locus_tag="PK683_11925"
     CDS             complement(1140..1340)
                     /locus_tag="PK683_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09200.1"
                     /translation="MIGLTMLINRQQVYSGTAPYGHFEFGWQGLRHGNFFHRLGGRVM
                     SFHDRYSSPVFAGVEYLVGYSL"
     gene            complement(1598..1828)
                     /locus_tag="PK683_11930"
     CDS             complement(1598..1828)
                     /locus_tag="PK683_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09201.1"
                     /translation="MKLFYLFVLFSVLPLFSAEIVLKDGSAFICDTVSENSMNVTVMY
                     KGQRYVIPRSEIESYQPEKKDLILPFPILYFS"
BASE COUNT          520 a          460 c          447 g          496 t
ORIGIN      
        1 ggtcgttccg ccgacaggat aatagtcata cagtgcagaa ggagtgatcg aagaggcagg
       61 aatgaagaca tttgtcgcag tgctccctcc gaacacaagc agtgcaggcg aatctgcagg
      121 atacggatcg gagggaagag gcctgtagca ggaggaggca gatccgtgtc ttgccccgtt
      181 cagactggct tctatcagag aagtcgtggt attcgcaccg ggtatgaatg catccgagga
      241 agactgttct gcaccgcttg aatacagacg tcctccagtc atgtaaattg tcccgtcgtt
      301 tccgcacccg gccatgtcaa ttctaggaag cagacttcct ccctgaaccg tgtactgcgt
      361 ccatgtgccg gtgcttccga cggaaggctg aaacctgtat actgtattca gtattgttcc
      421 tgtagtcata tcggtcgtgg tcgtcccgcc gatcatgtaa atttcattat tcatcttccc
      481 tgcaagcgca ccagtcaggc tgttcggcat tgaagaaagt gctgtccagg catttttatc
      541 caggtcgtac atctccactg cattggatac agagaaggat gttcctgaag ccttgatccc
      601 tccgatcaca taaatttttc cggaaatgac agtgactgat gcaaatgccc tcggagtcgg
      661 aagagaagtg actgaaggat accaggtgtt cgtggccgga tcatacatat ccacctgtgc
      721 gaccggaagt ccggaaactc ctattccgcc tatgatccag atgcttcttt tctttatgac
      781 aagaagagga tcaggattgg gagtgctgaa agactgagga aagactgtct tcggactcac
      841 tgaaccgcag aatacagcgt atgtatattt ctggtctgat gtcagtcctg caagtgatat
      901 gctgtgaatt ttcgaaggcg aaagacttgc ctggtatttc tgaatcgagc cgatgccgta
      961 tcccacaatc cctgtcactg attccgggca tgaaaatgta attactgcag actgcgaagt
     1021 cagcgagctt acataaagaa acagaagtct gactgaatcg ttcttctgag ccttctcaag
     1081 cggagatttc tggcagaagg aaagcagaag acaatacaga ataatgaaaa tttcctttct
     1141 cacaggctgt accccactaa atactccact cccgcaaata caggactgga atacctgtca
     1201 tgaaaactca tgactcggcc tccgagcctg tgaaaaaaat ttccgtgacg caggccctgc
     1261 catccgaatt caaaatgtcc gtaaggcgca gtgccggaat acacctgctg cctgtttatg
     1321 agcattgtga gtccgatcat atctggcgac gtgaacagaa ggactgtctg cgaaggatga
     1381 gagacgtagc ggatctgcga accgccgaat accgaaacgg tttcctgaag acgacgattc
     1441 ggactgccag actctgctca gttcaggttc cttcaccgga ggattttttc tcgaagcaat
     1501 ttcctttttc ggaatcacca tacaccaagc tgcgttttta ctgtgatgtc cctgtctgtc
     1561 tcttctgcca cagctccacg cagttcactt ctgtccttca gctgaaatac agaataggaa
     1621 aaggaagaat gagatccttt ttttccggct gatacgattc tatttcagat ctggggatga
     1681 catatctctg acccttgtac attacagtga cattcattga attttcgctg actgtgtcgc
     1741 agatgaatgc agacccgtcc ttcagcacaa tttcagcaga gaacagagga agaacggaaa
     1801 ataatacaaa cagatagaat aatttcatcc aatttcctcc aaatctcttt acaaaaacta
     1861 agaatagccg tttattgtat tttcaagaaa ggctagttat ataaaaacac ttttcgccac
     1921 ttt
//
LOCUS       DAOQGJ010000761         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41568_length_1922_cov_3.940546, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000761 DAOQGJ010000000
VERSION     DAOQGJ010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41568_length_1922_cov_3.940546"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..409)
                     /locus_tag="PK683_11935"
     CDS             complement(65..409)
                     /locus_tag="PK683_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNH09202.1"
                     /translation="MTNLPKCPECSSDLTYEDGEMFVCPECSHEWSANPAEENETESS
                     IVKDANGNVLQNGDSVSLIKDLKLKGSSSVLKAGTKVKNIRLTEGDHNIDCKLDGIGA
                     IQLKSEFVRKIQ"
     gene            complement(593..>1922)
                     /locus_tag="PK683_11940"
     CDS             complement(593..>1922)
                     /locus_tag="PK683_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH09203.1"
                     /translation="FWCSVTGQAINPENLDEGTIWVTEDVTERKNIEFLLQQKNAELE
                     KVNMELVHQKEEAVSASRLKSEFLANMSHEIRTPLNGVIGFAELLSSTALNENQHSLL
                     DNVRSSAGSLLSIINDILDFSKIEAGRLELESVRTDIRTLAEESLDTVRLQAEKKGLI
                     LELHIQDECPEFAYTDPLRLKQIILNLLNNAVKFTESGRVDFSLRYRMKNEIENIAEF
                     TFSVQDTGIGISKTERKKLFTAFSQADTSTARKYGGTGLGLVISSRILTAMNSILELK
                     SEEGKGSLFYFTVEFKMERGQRGRKDFSSETDNFSFNLQYDPVILIADDNLMNLALAR
                     RMTAKLIPSAVIIEARNGNEAVHNYVQFSPHLILMDVQMPGKDGYDATKEIREIEKKN
                     QTHTPILALTANAAAGERENCIAAGMDDFISKPFEIKNLKIILQKYLGKK"
BASE COUNT          465 a          421 c          348 g          688 t
ORIGIN      
        1 catttcaaaa atgaatgttt ccccataaag tcaatgatta tctttttatt atatgatgta
       61 aatactattg aatcttcctt acaaattcgg atttgagctg tattgcccca atcccatcca
      121 gtttgcagtc aatattgtga tccccttctg tcagtctgat gttcttcact tttgttcctg
      181 ctttcagaac agaagaagac cctttcagtt tcagatcctt gataagagag actgagtctc
      241 cgttctgaag aacgttcccg tttgcatctt ttacaatact gctctctgtt tcattttctt
      301 ctgcaggatt tgccgaccat tcgtgagagc attcgggaca tacaaacatt tctccatcct
      361 cataggtaag atctgaactg cattccgggc atttgggaag atttgtcatt gtggttcctg
      421 cttaattttt gtctgctgaa cttctttcgg ctgtcaatcc agcttttttt ctgctttttt
      481 ttcttccatt catcttttgg cagtcggata tttttatgat tttcctctgt ttgaaatatg
      541 attccgtttc gaacgttgat taatgcggaa ttgaagattc taatcgaacc tatcattttt
      601 tcccaagata cttctggaga atgattttca gattttttat ttcaaaaggt tttgaaatga
      661 agtcatccat tcctgctgct atgcagtttt ctctttcgcc tgctgcagcg tttgcagtca
      721 gtgcaagaat tggagtgtgg gtttggttct ttttctcaat ttcccgaatc tcctttgttg
      781 catcatatcc gtcttttcca ggcatctgaa catccattaa aatcaggtga ggggaaaatt
      841 gtacataatt atgcactgcc tcatttccgt ttctcgcttc gataatgacc gcactgggga
      901 tcagctttgc tgtcattctt ctggcaagtg cgaggttcat cagattgtca tcagcaataa
      961 gaataacagg atcgtattgc agattaaatg aaaaattatc cgtttcgctg ctgaaatcct
     1021 ttcgtcctct ctgccctctt tccatcttaa attcaacagt gaaataaaaa agacttcctt
     1081 ttccttcttc gcttttgagc tcaagaatac tgttcattgc agtcagtatt ctgcttgata
     1141 tcacaagacc gagtccagtc cctccgtatt ttcttgctgt agatgtatct gcctgcgaaa
     1201 atgcagtgaa taattttttt ctttcggttt ttgaaatgcc tatccctgta tcctgaacag
     1261 agaaggtaaa ttctgcaatg ttctcaattt cattcttcat tctgtatcgg agagaaaaat
     1321 cgactcttcc agactctgta aacttaactg cattgttcag aagattgaga ataatctgtt
     1381 tcagacggag aggatcagta tacgcaaatt cagggcattc atcctgtata tgaagctcca
     1441 gtatcagtcc ttttttctct gcctgcagtc tgactgtgtc cagactttct tcagcaagcg
     1501 ttctgatgtc agttctgaca gattccagtt caagccttcc tgcttctatt ttagaaaaat
     1561 ctaatatatc gttgatgatt gaaagaagcg accctgcaga gctgcggaca ttgtccagaa
     1621 gagaatgctg attttcattc agggccgttg aactgagaag ctctgcaaat cctatcactc
     1681 cgttcagagg agttctgatc tcatgactca tatttgcaag gaactcgctt ttcagcctgc
     1741 ttgcagatac agcttcctct ttctgatgaa ccagttccat attgactttt tcaagttctg
     1801 catttttctg ctggagaaga aattcaatat ttttcctctc agtcacatct tctgtgaccc
     1861 agatggttcc ttcatccaga ttttccgggt tgattgcctg tccggttaca ctgcaccaga
     1921 at
//
LOCUS       DAOQGJ010000762         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41588_length_1922_cov_2.427424, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000762 DAOQGJ010000000
VERSION     DAOQGJ010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41588_length_1922_cov_2.427424"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PK683_11945"
     CDS             complement(<1..613)
                     /locus_tag="PK683_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09204.1"
                     /translation="MKLSAKFLKRKWSSVLLFLRERLKIRSWMVSVFLAFFLLFSLVL
                     ISNNLLIYAVKQKYINIYKIRAHISEYFRNRQNKAIEIGLVDFSLIGGIIFEDLRISS
                     EEDFSNNRIFLKSERIEIRLQKIFSTAPEIKKVVFSNAHITLNAGDSDVDRLFDFLKE
                     VYIPEVEFTDLYVTVKDGKDILLDTKYPMSLVLKRKDRSFHVEY"
     gene            complement(615..>1922)
                     /locus_tag="PK683_11950"
     CDS             complement(615..>1922)
                     /locus_tag="PK683_11950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNH09205.1"
                     /translation="NRNLCVVGDDDQSIYGFRGSDRDLILNFERDFPGTKTIKLLQNY
                     RSCEPILKMANALIKNNQNRKEKELWSARKTGDLPLYTELGDEKEEASFVADKVQELI
                     LRHKKKGSEIAVLFRTNYQSRPFEEELRLRSIPYRLIGAYNFFDRKEVKDLIAYIRII
                     ANSKDDLSFLRILNYPKRGLGETAREKIQNKSHHDGISISEVLLRICESPDYIPDLKK
                     NAASSVYALMELISKYKKEFHSTLSLTHTLKNLVTEAGFEKEFFSEEKDAKVIKARMF
                     NLSELINMLDYFEKEWEGETRPTLFDFLLRLALLTSDDGDSEKEDSRIQLMTMHLSKG
                     LEFDAVFLCGLEEGFLPSARSMSETNDADEERRLFYVGITRAREKLFLSSCRNRKKFG
                     ESVPVQPSRFLSEIPEELMETAKTDDESAAFSFLENLESLKAV"
BASE COUNT          512 a          422 c          378 g          610 t
ORIGIN      
        1 tgtactcgac atgaaatgac ctgtcctttc ttttcaggac aagactcata gggtattttg
       61 tatccagaag tatgtctttg ccgtccttta ccgtaacata aagatccgta aattccactt
      121 cggggatata gacctctttc aggaaatcaa aaagcctgtc tacatccgaa tcgcctgcat
      181 tcagagttat atgggcgttg gaaaaaacaa ctttctttat ttcaggtgca gtggaaaaaa
      241 ttttctgaag acggatttcg attctttccg atttcagaaa aattctgttg ttggaaaagt
      301 cctcttccga ggaaattcgg agatcctcga atatgattcc gccgatcaga ctgaaatcca
      361 caagtccaat ttcaatcgcc ttgttctgtc tgtttctgaa atactcggaa atatgtgctc
      421 tgattttata gatatttatg tatttctgct ttactgcata aatcagaaga ttgttcgaaa
      481 tcagaacaag actgaaaagc agaaaaaaag ccagaaagac ggaaaccatc caggagcgga
      541 tcttcaggcg ctcccgaagg aacagcagga cagaactcca ttttcttttc agaaatttag
      601 cggataattt catttcagac tgctttcaga ctctccagat tctcaagaaa tgaaaaagcc
      661 gcagattcgt catctgtttt agcagtttcc ataagttctt caggaatttc tgaaaggaaa
      721 cgggaaggct gaacaggaac ggattctccg aattttttcc tgtttctgca ggaacttaag
      781 aaaagcttct ctcttgccct tgtaattccc acataaaaaa gacgtctttc ctcatctgca
      841 tcatttgttt cggacatact tctggcactg ggaagaaaac cttcctcgag gccgcagaga
      901 aataccgcat caaattccag tcctttggac aggtgcattg tcatcagctg tattctgctg
      961 tcttcctttt cactgtctcc gtcgtcgctt gtcagaagtg caagtctgag cagaaaatcg
     1021 aacagtgtag gtctcgtttc tccctcccat tctttttcaa aatagtccag catattgatc
     1081 agttctgaaa gattgaacat tctggccttg atgacttttg catccttttc ttcggaaaaa
     1141 aattcttttt caaagcctgc ttccgttacc agatttttga gagtatgcgt cagagacaga
     1201 gtggagtgaa attctttctt atactttgaa atcagttcca tcagtgcata taccgaggat
     1261 gctgcatttt ttttcagatc aggaatatag tcaggacttt cgcagattcg cagaagcact
     1321 tctgaaatgg agattccgtc atggtgagac ttgttctgaa ttttttctct tgcagtttcg
     1381 ccgagtcccc gcttgggata attcagaatg cgcaggaagg acagatcatc cttggaattt
     1441 gcaatgatcc tgatataggc aatcagatcc tttacttcct ttctgtcgaa gaaattataa
     1501 gcgcctatca gtctgtatgg aattgatctg agcctgagtt cttcctcgaa aggtctggac
     1561 tgatagtttg ttcgaaagag aactgcaatc tcgcttccct tttttttatg acgaagtatg
     1621 agttcctgaa ccttgtctgc cacaaaggaa gcctcttcct tctcatcccc cagttcagtg
     1681 tacagaggaa gatctcctgt ttttctggcc gaccagagtt ccttttcctt cctgttctga
     1741 ttgtttttta taagtgcatt tgccatcttc agtatgggct cgcaggatct gtaattctga
     1801 agaagcttga ttgttttcgt accgggaaaa tccctttcaa aattcagaat cagatccctg
     1861 tctgaacctc tgaaaccgta tatgctctga tcgtcgtctc ctaccacaca gagattcctg
     1921 tt
//
LOCUS       DAOQGJ010000763         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41719_length_1918_cov_3.830381, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000763 DAOQGJ010000000
VERSION     DAOQGJ010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41719_length_1918_cov_3.830381"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="PK683_11955"
     CDS             <1..1502
                     /locus_tag="PK683_11955"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNH09206.1"
                     /translation="ENWLNEAIAETYIPLLKAFQHLKRDGVPFKITMTFTPTLVSMLT
                     DRYLQENFLKYIDRLIELSDKEIQRTKYDPHLNYLSQYYHDRFLETKKLFLSLDKNII
                     NGYLEFYKDGSLEVITCNATHGFLPLLESEPSIVRAQIALGRREHKLTWGIEPRGIWL
                     AECGYFKGVEKYLAEEGFRYFFVDTHGIMNAEPRPRFGTYAPVEIGEGIFAFGRDKES
                     SEQVWSAEQGYPGDYRYREYYRDIGFDLDYDYIKDYVHPTGIRYNTGIKYHRITGKTA
                     YKEYYQPDWAKEAAGNHAEDFLRSRIAQAKAFREKEGQPCVIVSPYDAELYGHWWFEG
                     PMFLEYLFRKAHFDQSDIRFAQPMDVIGNLSRIQSVSMDSSSWGENGYSDVWLNSSNE
                     WVYKHLMECSISIHELASIYKGTEDDIKRRILNQMGREALLLQSSDWPFIMKTGTVVQ
                     YAEKRVRVHTNLFQELKEMLISGNVNEDRLKHIEEEHPIFPELNFEIFC"
     gene            1486..>1918
                     /locus_tag="PK683_11960"
     CDS             1486..>1918
                     /locus_tag="PK683_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HNH09207.1"
                     /translation="MRYSAEMESISLFKHCLFDIEGTIAPISFVHDILFPYSKAKMQT
                     YLEKENLTDSVWQSIQSEYAADLKTGSFQDALERNDISSIYHYIEFLIKTDRKNSGLK
                     EIQGHIWQKGYESGEIKSTIFPDTVQFWEKLQTSFTGISIYS"
BASE COUNT          610 a          379 c          433 g          496 t
ORIGIN      
        1 aggaaaactg gctgaatgag gccattgcag aaacgtatat tcctcttctg aaagcatttc
       61 agcatctgaa gcgcgacgga gtgcctttta aaatcacaat gacatttact cctactctgg
      121 tttccatgct gacagacaga tatcttcagg aaaattttct gaagtacatc gaccgtctga
      181 ttgaactttc agacaaggaa attcagagaa caaagtatga ccctcatctg aattatcttt
      241 ctcagtacta ccatgacaga tttctggaaa caaagaaact ttttctttct ctcgataaga
      301 acattatcaa cggatatctg gaattctaca aagacggaag cctggaagtc atcacctgca
      361 acgcaactca cggattcctt cctcttctgg aaagtgaacc ttcgattgta cgtgcacaga
      421 ttgctctggg aagaagggaa cacaaactca cttggggaat tgagcctaga ggaatctggc
      481 ttgccgagtg cggttatttt aaaggcgtgg aaaaatatct ggcagaagaa ggattcagat
      541 atttctttgt agacactcac ggaattatga atgctgaacc gagaccgcgc tttggaacat
      601 acgctccagt ggaaatcggt gagggcattt ttgcatttgg aagggacaag gaaagttccg
      661 agcaggtttg gagtgcagaa cagggatatc ctggagatta cagatacagg gaatactaca
      721 gggatatagg atttgatctg gactatgatt atataaagga ttatgttcac ccgactggaa
      781 tcagatacaa tacaggaatc aagtaccaca gaattacagg aaagactgcc tacaaggaat
      841 actatcagcc ggactgggca aaagaagcag caggaaatca cgcagaggat ttcctgagaa
      901 gcagaattgc acaggcaaaa gctttccgtg aaaaagaagg acagccctgc gtaatcgtat
      961 ctccttatga cgccgagctt tacgggcact ggtggtttga aggaccgatg tttctcgaat
     1021 accttttcag aaaggctcat tttgatcagt ctgacatcag attcgctcag ccaatggatg
     1081 tcatcggaaa cctgtccaga atacagagtg tatctatgga ttccagcagc tggggtgaaa
     1141 atggatattc tgatgtatgg ctgaactcat ccaatgaatg ggtatacaaa catcttatgg
     1201 aatgttccat ttcgattcac gaacttgcca gcatttacaa gggaaccgaa gacgacatta
     1261 agagaagaat actgaatcag atgggaagag aggcacttct tctgcagagt tccgactggc
     1321 ctttcattat gaagacgggt acagtcgtgc agtatgcaga gaaaagagtc agagtccata
     1381 caaatctttt ccaggaactg aaagaaatgc tcatatcagg aaatgtgaat gaagaccgcc
     1441 tcaagcatat tgaggaggag catccgattt tccctgaact gaattttgag atattctgct
     1501 gaaatggaaa gcatcagtct cttcaaacac tgtctgtttg acattgaagg gacgattgca
     1561 cctatcagtt ttgtacacga tattcttttt ccatattcaa aagctaaaat gcagacatac
     1621 ctggaaaaag aaaatctgac tgattctgtc tggcagtcca ttcagagtga atacgctgcc
     1681 gacttaaaaa ccggcagttt tcaggacgct ctggagcgta atgatatttc atccatttat
     1741 cattatattg aatttcttat aaaaacagac cgaaagaact cagggttgaa ggaaattcag
     1801 ggacatatct ggcagaaagg ttacgaatca ggagaaatca aaagcacaat tttcccggat
     1861 acagttcagt tctgggagaa actgcagacc agttttaccg gcatttctat ttactcct
//
LOCUS       DAOQGJ010000764         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41738_length_1918_cov_2.815888, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000764 DAOQGJ010000000
VERSION     DAOQGJ010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41738_length_1918_cov_2.815888"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1276
                     /locus_tag="PK683_11965"
     CDS             56..1276
                     /locus_tag="PK683_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH09208.1"
                     /translation="MKKIKTAAVALICAGILWAGDSESPKAVLSLDQSFSLSAGQSKS
                     IDLKVGVPTGYGIVVGHSGKSLNMIANFGQSAVSGLHFSIEKMPEGIRKGNNLLAAGN
                     AVFKVRLSELKGAKAGSSLQSEIVFNYQLCPLSSGKCLSPDGITKSVTAVISKDRELK
                     LAASKSNSASPIKWAGTYENALKTAKSSGQNIYVIVTAPSWCGACQMMERETFAKANV
                     QKVLNSDFVSLQVLDTSSDMEKFNIEGFPTSFVLDSQGNELSQQVGGIDASQFLSFLS
                     DFKKTKKGDDDSDDSDPVPHKDATDKKITWEVRKKTTHSGGADWSGFLGTMTGTKEEC
                     KQVCAADDECHAFFIVKRGQLDIPPKGPNKYWKHFRPGECVFLHGRPSSADVKQSDMY
                     YKKVNGKKAYSIGD"
BASE COUNT          550 a          379 c          442 g          547 t
ORIGIN      
        1 ctaaaaagaa ttgaaaaatc agaaactgtt ctcattttga aaataggaga atttaatgaa
       61 gaaaataaaa acagcagcgg ttgcattaat atgtgcaggc atactttggg caggagacag
      121 tgaaagtcca aaagcagtac tgagtttgga tcagagcttt tcactgtcgg cgggacagtc
      181 caaatcaata gatctcaaag tgggtgttcc tacaggatac ggaattgttg tcggtcacag
      241 cggaaagagt ctgaatatga ttgcaaattt tggtcagtca gctgtaagcg gactgcattt
      301 cagtatagag aaaatgcctg agggaatcag gaaaggtaat aatttactgg cggcaggaaa
      361 tgcagttttt aaagtccgtc tctctgaatt gaaaggagcc aaggcaggtt cctctctgca
      421 gtcggaaatc gtattcaatt atcagttgtg ccctctttca tcaggaaaat gtctttctcc
      481 ggacggaatt acaaaatcag tcacagcagt aatttcaaag gacagagaac ttaagcttgc
      541 cgcatcaaaa agcaattctg cttctcctat caaatgggcc ggaacatacg agaatgcact
      601 gaaaacggca aaatcctcag gtcagaatat ttacgtgatt gtcacagctc cttcctggtg
      661 cggggcttgt cagatgatgg aaagggagac ctttgcaaag gcaaatgttc agaaagttct
      721 gaactctgat tttgtttctc tgcaggtgct cgatacaagt tctgatatgg aaaaattcaa
      781 cattgaagga tttcctactt cctttgtgct ggattcgcag ggcaatgagc tttctcagca
      841 ggtcggcgga atcgatgcat cgcagttctt aagtttcctt tctgatttca aaaaaacgaa
      901 aaaaggcgat gatgacagtg atgattctga tccggttccg cataaggatg ccacagataa
      961 aaaaatcact tgggaggttc gtaaaaaaac aactcattca ggcggagcgg actggtcggg
     1021 ctttttagga acaatgaccg gcacaaagga agaatgcaag caggtctgtg ctgctgatga
     1081 cgaatgtcat gcgttcttca tagtaaagag agggcagctc gatattcctc caaaaggacc
     1141 caacaaatac tggaaacatt tccgtcccgg tgaatgtgtt tttctccacg gaagaccgtc
     1201 ctctgcggat gtcaagcagt cggatatgta ttataaaaaa gtaaacggta aaaaagctta
     1261 ttctatcgga gactgatctt cgaaactttt tcattcaggc ggctatattc ggctgactct
     1321 gcatcttagc tcatgtgtga aggtgagcta agcaatgatc ttgactttct tttcgatccc
     1381 cgagatctca atgctgtaca attagctttc atatctgtta tatggaatga tacctcgact
     1441 tgtgctgagc gatgcccttg agctgtgtag aagggtcata tcgaagcatg caaacccaat
     1501 attttcaggg tttttcgtat ggattcgatc aacttacaac aagtcgaggt gggcggtgaa
     1561 aatcatggtt cggtatactc ctgcaccgct cgccaaccgt ttcttctgaa ttaagaacct
     1621 taatgtttca gtatcttccc ggcttgcgtt ggtttcgcaa acatacagcc gttaaaattc
     1681 ctgctttttg cctggctgcc aggcgacatt cagtgtcgac tggaattgac ttttaaccaa
     1741 tacgtcattg atcttattta ctcaccttac gcaggtgggg cgtttttggc ataagcctac
     1801 cggcggtttt aagggcttcg cccttaaaaa tcctgttttt actgattcat tagattttcc
     1861 taatgctttc ggaaaattat tgcatctaga tagcatattg tatttgcgta aggtgagt
//
LOCUS       DAOQGJ010000765         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41781_length_1917_cov_2.767454, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000765 DAOQGJ010000000
VERSION     DAOQGJ010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41781_length_1917_cov_2.767454"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PK683_11970"
     CDS             complement(<1..690)
                     /locus_tag="PK683_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNH09209.1"
                     /translation="MRQFQEFKKKYPDSIIFFRLGDFYEMFMDDAVTASRILDIALTK
                     RQNQIPMCGIPFHAADNYLSRLISAGQTVVICDQIKSDEPAGKVLQREVVRIVSPGTV
                     LEDNLLKSYENNYLCFIYISFSVISVLFADITTSENSYYLIEKLDSEKLDSVLKKFNP
                     SEIVFFREHSLSWSNLGLNVKCHITLLDSSQYNFPESGDYESMRSVLEELLGAYFKNS
                     QFRFPKAEEIRD"
     gene            complement(701..1324)
                     /locus_tag="PK683_11975"
     CDS             complement(701..1324)
                     /locus_tag="PK683_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNH09210.1"
                     /translation="MKVKVCGIKDIETALFAAEEGADFIGLNFSPESKRKTDISTAEK
                     IIFHLRKNFEKKTEIVLLFFRNSEEDIRNALSSLEYDRVQFVARDETWKNPGLDKNIL
                     LPQISVEKEIYDSDLDAESDLLILDSHHKTLGGGTGTVFPWAYVKHLKRKYLLAGGIT
                     PKNLNLVRSELNPFGVDTASGVESSPGVKDRNLIREFIRNAKGISAP"
     gene            complement(1494..>1917)
                     /locus_tag="PK683_11980"
     CDS             complement(1494..>1917)
                     /locus_tag="PK683_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09211.1"
                     /translation="DVGSAHIVAAENTTGAYPHVSPESGAQEAIQKAVERIYPKVREQ
                     FVSKWTPGTLIRVVIEGLDYDTFLDKDIQGQIRKIKGVNNVSIKGNSNVNKMIPMEVK
                     AMFNGVTLYRKMRERKGDMGIEFTQKEVKPGNLHLIVK"
BASE COUNT          507 a          447 c          386 g          577 t
ORIGIN      
        1 atccctgatt tcctccgcct taggaaaacg aaactgactg ttcttgaaat aggcaccgag
       61 aagttcttcg agaacggaac gcatcgactc atagtccccc gattcaggaa aattgtactg
      121 agaagaatca agcagagtaa tatgacattt tacattcagt ccaagattgc tccaggataa
      181 tgaatgttcc cggaagaata caatctcgga aggattgaat tttttcagta cggaatccag
      241 tttttcagag tcgagttttt caatcagata ataggaattt tcagaagtgg tgatgtccgc
      301 aaacagaact gatatgaccg aaaaagaaat ataaataaag caaagataat tattttcata
      361 acttttcaga agattgtctt cgagaactgt tccgggactc actatcctca caacctctct
      421 ctgaagcacc tttcctgcag gttcgtccga cttgatctga tcgcagatca ctacagtctg
      481 ccctgcagaa atcagcctcg aaagataatt gtccgcggca tgaaacggta tcccgcacat
      541 cgggatctgg ttctgtcttt tcgtcagtgc aatgtctaga atccgggaag cagtgactgc
      601 atcatccata aacatttcat agaagtcccc cagtctgaaa aatatgatgg aatcaggata
      661 tttttttttg aactcctgaa actgcctcat tgcaggagta ttaaggggct gagattcctt
      721 ttgcatttcg aatgaattcc cgaatcagat ttctgtcctt gactccggga gaggattcca
      781 caccgctcgc agtgtctacc ccaaatggat tcagttcaga tcgtacaaga ttcaggtttt
      841 taggagtgat ccctcctgca agcagatatt ttcttttcag atgttttaca taggcccaag
      901 gaaagacagt tccagttcct ccgcccaatg tcttatgatg gctgtcaaga atgagcagat
      961 cagactctgc atccagatcg gaatcataga tctctttttc tacggaaatc tgggggagaa
     1021 gaatattctt gtccagtcca ggatttttcc aggtttcatc tcttgccaca aactgcaccc
     1081 tgtcatattc cagagaagag agagcattcc tgatatcctc ttccgaattg cggaagaaca
     1141 gaagaactat ctctgttttt ttttcgaagt tttttctgag atgaaaaata attttttctg
     1201 ctgtagaaat atccgttttt cttttacttt cgggggaaaa attcagaccg ataaagtcgg
     1261 ctccctcttc ggcagcgaag agagccgttt ctatatcttt gattccgcaa acttttactt
     1321 tcattcggca ttaagtctgc cctgcagact gcaggacaaa gctttttgcg gcagggcaac
     1381 ggtcaaatca agacgccgca gagacaatat tgcggatcgc tttgcgatcc gaggcatcct
     1441 accggaggtt caaagggact tcgtcccttt gatatccctg ctgtatgcaa aatttatttc
     1501 acgatcagat ggaggtttcc aggcttgact tctttctgag tgaattcaat tcccatatcc
     1561 ccttttcttt ctctcatctt tctgtacaga gtgactccgt tgaacattgc cttcacttcc
     1621 ataggaatca tcttgtttac attagaattc cctttgatgc tcacattgtt tacgcccttg
     1681 atcttccgga tctgtccctg aatgtccttg tccagaaatg tatcatagtc cagtccttcg
     1741 atgacgacac gaatcagagt gcctggagtc catttggaaa caaactgttc tctcactttc
     1801 ggataaattc tttccactgc tttctggatt gcttcctgcg caccgctttc cggacttaca
     1861 tgaggatagg ctcctgtcgt attttccgca gcaacgatgt gggcagaacc cacatcc
//
LOCUS       DAOQGJ010000766         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41830_length_1916_cov_2.023643, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000766 DAOQGJ010000000
VERSION     DAOQGJ010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41830_length_1916_cov_2.023643"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PK683_11985"
     CDS             complement(<1..326)
                     /locus_tag="PK683_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNH09212.1"
                     /translation="MKDRIFIDTNIFLYSILDEKTKDKENERKAKAAKALIESINENS
                     ELVVSAQILNEIYVNLLKHKVAEKNIISSINSIVDSCNQLVSIDLKLLQVGWELKQTY
                     KLSYWD"
     gene            complement(316..546)
                     /locus_tag="PK683_11990"
     CDS             complement(316..546)
                     /locus_tag="PK683_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09213.1"
                     /translation="MNQAELKLEIFRQIDSLSDEKLIEVQKKILQILDVPDKSKGKKS
                     SANNHLKLKKLVQNPLRVSKINLLSRSEIYER"
     gene            complement(549..>1916)
                     /locus_tag="PK683_11995"
     CDS             complement(549..>1916)
                     /locus_tag="PK683_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNH09214.1"
                     /translation="GITEDSISVNNVEPQNQFYFSEIKFYNLGQFSQVIDDFETIHYN
                     SAPQSKLNNFLNFIVYAAKDYYPEGWLNNTFKTPNAVTIMTVHQAKGLEYPVVFIPGM
                     NRNYFPTKGQGGASVWSKFKEEIPLFVQNYERYKGDKENNEDERRLFYVALTRSQKFL
                     YVSRAPVPEKNLYQKESVFCKEISHADYIYTDINPNYSARPTAAPEPKKETANIFLNF
                     SLLKDYFECPYRFKLSSMYGFIQPLNMRLGHGKAVHNTLMDIHRQFLDGKQVSLDQID
                     GLLDIHYYLPYANPEGNVYESMHQSAKESVEIYYKENKDSFKDIEFAEKDIEIPLGDG
                     ILVNGRIDLVKKKNLDGTVETTIIDFKSAKDAQTFSISMDQLMLYAMGYKELTGERAD
                     FLSVYNLDENRPHKQKLEDSHLDTTRENIISAANKIRSNEFTKTSAPAHCESCRQSRV
                     CSKRR"
BASE COUNT          531 a          372 c          334 g          679 t
ORIGIN      
        1 ctatcccaat aagacaattt atacgtttgt tttaattccc agccaacctg taacaatttt
       61 aaatcaatcg agactagctg gttgcatgaa tcaactatag aattgatgga gctaataata
      121 tttttctctg ctactttatg ctttaggaga tttacataaa tctcattcaa aatttgcgca
      181 ctgacaacta gctcggaatt ttcatttatt gattcaatca aagcctttgc tgcttttgct
      241 ttccgttcat tttctttatc tttcgttttt tcatccaaaa tagaataaag aaatatattt
      301 gtatcaataa atattctatc tttcatatat ctcacttcta gataaaagat taattttgct
      361 gacacgcaaa ggattttgca ctagcttttt gagctttaaa tggttgtttg cggaagattt
      421 tttccctttg cttttgtcag gaacatctaa aatttgaaga atcttcttct gaacttcaat
      481 cagcttttca tcggataaac tgtctatttg tcgaaaaatt tctagcttta attctgcttg
      541 gttcattttc atctcctctt gctgcagact ctgctttgtc tgcatgattc acagtgtgca
      601 ggggcggatg tttttgtaaa ttcatttgag cggattttat tggcggcaga aatgatattt
      661 tcgcgggttg tatccaggtg tgaatcttcc agcttttgtt tgtgcggtct gttttcgtcc
      721 agattgtaga cagatagaaa atcagcgcgt tcgccggtta attccttata tcccatagcg
      781 tagagcataa gctgatccat cgaaatggag aacgtctgag catcctttgc ggatttgaaa
      841 tcaatgatcg tggtttccac tgtcccatcc agatttttct ttttgaccag atcaattctg
      901 ccgtttacta gaattccatc tcccaaagga atctctatgt ccttttctgc aaattcgata
      961 tccttgaatg aatccttatt ttctttatag taaatttcta cagattcctt ggcactctga
     1021 tgcatactct catacacatt tccttccgga tttgcatagg gaagataata gtgaatatcg
     1081 agaagtccgt ctatttggtc taaagaaacc tgttttccat ccagaaattg tctatgtata
     1141 tccatcagag tattgtgaac agccttgcca tgaccaagcc gcatattcaa tggctggatg
     1201 aatccataca tactcgaaag tttaaaacgg taaggacatt caaaatagtc tttcagcaga
     1261 ctgaagttta aaaaaatatt tgctgtttct ttcttcggtt caggtgctgc agtcgggcgt
     1321 gcagaataat tcggatttat atctgtgtaa atataatcag catgacttat ttctttgcag
     1381 aatacagatt ctttctgata cagattcttt tcaggaacag gtgctctaga gacgtaaaga
     1441 aatttttgcg accgagtgag agcaacataa aacagtcttc tttcgtcttc gttgttttct
     1501 ttgtctcctt tatatcgctc ataattttga acaaagagcg gaatttcttc cttgaatttt
     1561 gaccatacag acgcaccgcc ctgtcctttg gttggaaaat aatttcgatt cattccagga
     1621 atgaagacaa caggatattc cagacccttt gcctgatgca cagtcataat cgtaactgca
     1681 ttcggagtct tgaaagtatt gttcagccac ccttcaggat aatagtcctt tgctgcatat
     1741 actatgaaat tcaaaaaatt attcagtttg ctctgaggag cactgttgta atgaatcgtt
     1801 tcaaaatcat caattacctg actgaactgc cccagattgt aaaatttgat ttcagaaaaa
     1861 tagaattggt tctgcggttc aacattgttc acggaaatgg aatcctcagt tatccc
//
LOCUS       DAOQGJ010000767         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41874_length_1914_cov_3.917698, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000767 DAOQGJ010000000
VERSION     DAOQGJ010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41874_length_1914_cov_3.917698"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PK683_12000"
     CDS             <1..895
                     /locus_tag="PK683_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH09215.1"
                     /translation="LADLAAAAEETRSGVNTITDSNQALSQGSSEQAASIEEISSTVT
                     ELSAQAKQATVSVRQANEISFNSLESSKHGSEMMEKMMNAMQEIKDASDNISKILASI
                     DEIAFQTNLLALNAAVEAARAGRYGKGFAVVAEEVKSLSGRSANAAKETAVLIQESIQ
                     KANKGVEIASRTVKALDEISSSVKNVTGLMTEVSKASEEQMIALSEVEKSIQQIEQVI
                     QNNAFSSEETASASLQLSEQAENISKAIRRFRLPAKLLEQKRSQSSERRTRTQNKLGN
                     RNHPGNGSVKNPSRIISLGES"
     gene            973..1479
                     /locus_tag="PK683_12005"
     CDS             973..1479
                     /locus_tag="PK683_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH09216.1"
                     /translation="MKNNDFIDDEYDDEEEDTLQDMYLIFCCADKYYSVEVRYVSEIV
                     ALQSVTEIPDMPSYFKGVINLRGKILPVLDMRLRFGFSEMKYHERTCCIVSDKFGLIV
                     DSVTEVARIPASEITGAPMGDGISHQYVSGVSRSAEGLRIILNINSLLNSSDGIEAVH
                     SSDIRVME"
     gene            1482..>1914
                     /locus_tag="PK683_12010"
     CDS             1482..>1914
                     /locus_tag="PK683_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH09217.1"
                     /translation="MDSFLNEFISESFEDLKKIESCIVSIENCLNSDIVPDPEMVNTL
                     FRHMHSIKGSSGFFEMKEIVRLAHQAETVLDLFRKESLAITSDRLDLVLKARDQIQFL
                     FESVEKNGSDRGSEEITDSVILELKELEKFSEEKKPKKELKR"
BASE COUNT          602 a          355 c          473 g          484 t
ORIGIN      
        1 tctggctgat cttgcggctg ctgctgagga aacaagatcc ggtgtaaata caattactga
       61 ttccaatcag gccctttctc agggttcaag tgagcaggcg gcctccatag aagagatttc
      121 ttctacagtg actgaacttt ctgcacaggc aaagcaggcg actgtcagcg taaggcaggc
      181 aaatgaaata tcattcaatt cactggaaag ctccaaacac ggaagtgaga tgatggagaa
      241 gatgatgaat gcaatgcagg aaattaagga tgcatcagac aatatctcca agatacttgc
      301 ttccattgat gagattgcat tccagacaaa tctgcttgca ctgaatgcag cagtggaagc
      361 agccagagcc ggcagatatg gaaaagggtt tgctgttgtg gcagaggaag tgaagagtct
      421 gtcgggaaga agtgctaatg cagccaagga gactgctgta ctgattcagg aatcgattca
      481 gaaagcaaac aagggtgtcg aaattgcatc cagaactgtc aaggcactcg atgagatcag
      541 ttcgagcgtg aaaaatgtta ccggtctgat gactgaagta tccaaggctt ccgaggaaca
      601 gatgattgcg ctttcagaag tggaaaaaag cattcagcag attgagcagg taatacagaa
      661 taatgcattc agttcggaag agacagcatc ggcaagtctt cagctttctg aacaggcgga
      721 aaatatcagc aaggcaatca gaagattcag gctgccagcc aagctgctgg aacagaaaag
      781 aagccagagt tcagagcgca ggacaagaac tcagaataaa ctcggaaatc ggaatcatcc
      841 agggaacggt tctgtgaaaa atccatcccg catcatatcc ttgggagaat cctgatatat
      901 gtctgaaaga aatgcaggag atgcagtata tgaaatatga aataatgaca aatcagtcaa
      961 gaggatctta aaatgaagaa caatgacttt attgatgatg aatacgatga tgaggaagag
     1021 gatactctgc aggatatgta tctgattttc tgctgtgcag ataaatacta ttccgtcgag
     1081 gtcagatatg tttcagaaat tgttgcgctt cagtctgtaa ccgaaattcc tgatatgcct
     1141 tcttatttca agggagtgat caaccttcgc gggaaaattc ttcctgtgct ggatatgagg
     1201 ctccgtttcg gtttttccga gatgaaatat catgaaagaa catgctgtat tgtatcagat
     1261 aaattcgggc ttatagtgga ctctgtcact gaggttgcaa gaatccctgc ttcagaaatt
     1321 acgggagcac cgatgggcga cgggatctct catcagtatg tcagcggtgt ttccaggtct
     1381 gcggaagggc tcagaattat actgaatata aactcgcttc tgaattcatc cgacggtatt
     1441 gaggctgtgc actcatctga tatcagggtt atggaataat tatggactca tttctgaatg
     1501 aatttatatc agagtcgttt gaagacctta agaaaataga atcctgcata gtctctatcg
     1561 aaaactgtct gaattctgat attgtacctg acccggaaat ggtcaatact ctcttcaggc
     1621 atatgcacag cattaaaggg tcgtccggtt tttttgagat gaaggaaatt gtccggcttg
     1681 ctcatcaggc cgagactgtt cttgatctct tccgaaagga aagtcttgcc attacctcag
     1741 accgtctgga tctggtactg aaggcaaggg atcagattca gttccttttt gaatctgtgg
     1801 aaaaaaatgg cagtgacaga ggaagcgagg agattaccga ttctgtcatt ctggaactga
     1861 aagaactgga aaaattttca gaggaaaaga aaccaaaaaa agaattaaag agat
//
LOCUS       DAOQGJ010000768         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41895_length_1914_cov_2.151694, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000768 DAOQGJ010000000
VERSION     DAOQGJ010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41895_length_1914_cov_2.151694"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PK683_12015"
     CDS             complement(<1..554)
                     /locus_tag="PK683_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09218.1"
                     /translation="MVLILLLFSTAVFAESRFTVDSFGFGTRVREDRSAPDLRSENGF
                     YLIPGISGKTDSGRAYSGEFGARILFRSGKADLFFSENSYVNAGFGKFGICAGRRPYS
                     EKFIRSAWTDGTEGIWIYYRFNDRIMMNLSAFDWYRGFPLLENDLLKKTADPDFSSGR
                     RQRNGLRIYYNDEKMTAGISLQYL"
     gene            complement(545..>1914)
                     /locus_tag="PK683_12020"
     CDS             complement(545..>1914)
                     /locus_tag="PK683_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09219.1"
                     /translation="GSFHSVIFQDSRMILHPETNSGNFLSEFSDFNSASPGEKNPEPL
                     ISRASVSEVNPFGSYMNGVSVSEDRFIELKAESPGSAELVFEKNSVPVKWIFYLRNSL
                     YETLSSGSLQCFPEVPTIYDPDLKISKELKNIALNGIRSDIPDLETAGSDERTPLKKR
                     ASAVNTFLRSEWTVSVSSQNLRMDERCRAETFASPGAENSWSSFLRAENGKLYIYSLI
                     NIYETAEVSVQSYFPSVSSLLNLPVFYDSGQPYFRESDAASASPSVQYLSIPPSGRSA
                     LLNFSGLYIEAVFPNPENQNDEWVLLCSRGNSEFPTAGLEIQDENSSDMLIPYLQRFG
                     SIPPELNASEFSSDSVIRPGQCAYILDPDTVSPKIRPYGALPTLLLTVSGSGNSTIGN
                     GISNDENLDLFQNISGTRIHLHSYGNRFDAGRFRISAEKNEAVILKKDRTGSSASDYR
                     KEIWF"
BASE COUNT          542 a          438 c          391 g          543 t
ORIGIN      
        1 ttcagatact gaagagaaat tcctgcagtc atcttttcat cattataata tattcttaat
       61 ccgtttctct gtctcctgcc ggaagaaaaa tcaggatcag ctgtcttttt cagcagatcg
      121 ttctcaagaa gaggaaatcc cctgtaccag tcgaatgccg atagattcat catgattctg
      181 tcattaaatc tgtaataaat ccagatccct tctgttccgt ctgtccatgc tgaacggatg
      241 aatttttcac tgtacggtct gcgtcctgca cagattccga attttccgaa acctgcattt
      301 acataggaat tctcagaaaa gaaaagatcg gcctttccgc ttctgaaaag aatccttgca
      361 ccgaactctc ctgagtatgc tcttccgctg tctgtctttc cggaaattcc aggaatcaga
      421 taaaaaccgt tctccgatct gagatcagga gcagatctgt cttcgcgaac cctggttccg
      481 aatccgaagg aatcaacagt aaacctgctt tccgcaaaga ctgcagtgct gaaaaggaga
      541 agaatcagaa ccatatttcc ttcctgtagt cggatgcaga tgagcctgtt ctgtcttttt
      601 tcaatatgac agcctcattc ttttctgcac tgattctgaa cctgcccgcg tcaaatctgt
      661 ttccgtaaga atgaagatga attcttgttc cggaaatatt ctgaaacaga tccagattct
      721 catcatttga aattccgttt ccgattgtac tgtttcctga accgcttaca gtgagaagca
      781 gagtcggcaa agccccgtaa ggccttatct tgggtgaaac agtgtccgga tccagaatat
      841 aggcacactg ccctggacgg atcaccgaat cagaactgaa ttcagatgca ttcagttcag
      901 gaggaatgct gccgaatctc tgcagatacg gaatcagcat atctgaagaa ttttcatcct
      961 gaatttcaag ccctgccgtc ggaaattctg aatttcccct cgaacacaaa agcacccatt
     1021 catcattctg attttccgga ttcggaaaaa cagcctctat gtaaagacct gaaaaattga
     1081 gaagcgcgga tcttccagaa ggcggaatgc tcagatactg gacagaggga gaagcagaag
     1141 cagcatccga ttccctgaaa taaggctgtc cgctgtcgta aaataccggc agattcagca
     1201 gagaggacac ggatggaaaa taagactgaa ctgaaacctc tgcagtttcg tatatattta
     1261 ttaatgaata aatgtacagt tttccgtttt ctgccctgag aaagctgctc cagctgttct
     1321 ctgcaccggg acttgcaaaa gtctctgccc tgcatctttc atccattcgg agattctgag
     1381 aacttacaga tacagtccat tcggatctga ggaaagtatt cactgcactc gctctttttt
     1441 tcagaggagt cctctcgtca gatcctgcag tttccagatc gggaatatcg gaacggattc
     1501 cgttcaatgc aatatttttt aattcttttg agattttcag atctggatca tatattgtcg
     1561 gaacttccgg aaaacactgc agacttccgg aagaaagggt ttcatacaga ctgtttctca
     1621 gatagaatat ccattttaca ggaacagaat ttttttcgaa aaccagttcg gccgatccgg
     1681 gactctctgc tttcagttca atgaatctgt cctctgaaac tgagacaccg ttcatatagg
     1741 aaccgaaagg attgacttca gaaacggaag ctctggaaat gagaggttca ggatttttct
     1801 ctcccggact tgcactgtta aagtcggaaa actctgaaag aaaatttcct gaattcgttt
     1861 caggatgaag gatcattctg ctgtcttgaa aaatgactga atgaaatgaa cctc
//
LOCUS       DAOQGJ010000769         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41918_length_1913_cov_3.347147, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000769 DAOQGJ010000000
VERSION     DAOQGJ010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41918_length_1913_cov_3.347147"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PK683_12025"
     CDS             complement(<1..1434)
                     /locus_tag="PK683_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH09220.1"
                     /translation="MAEYPVPENEAGRLKTLYGFDILDTLPEDQYDGITKIISEICQV
                     PVALICLIDENRQWFKSKIGLDIQETPRNISFCQYAIMDDGLLTVEDAERDIRFKNNP
                     LVVDSPRIRFYAGMPLRTTDGFNIGTLCVMDSKPKILSPSQKDALSAMAKQVVHLLEL
                     KKANQALEKTQKTISNFFKLSLDLLCIANEKGYFININPSFEKILGYSENELLSRPFI
                     DFVHPDDVNDTLNVMNRLNQGEDCTDFINRYRKISGEYIYISWHATTDMDTGLIYAAA
                     RDITEEMRHSREIYDLNSALEQSAIVARTDHRGIITYVDDNFCRISKYAREELLGKNH
                     RIVNSGYHSKEVFKNMWETITSGKVWKGEIKNRAKDGTYYWVDATIVPLRDVETNRQH
                     YIAVRYEITDKKEYEEKLKETNDRLNQAIRFKDIFLANMSHEIRTPMNAIIGFSNLLS
                     NTELNHEQKEYLHSITVASKNLLVIIND"
     gene            1594..>1913
                     /locus_tag="PK683_12030"
     CDS             1594..>1913
                     /locus_tag="PK683_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH09221.1"
                     /translation="MNEISEWIQNASDGDKFSIARLISLIENKFSFENRKKVFSILDR
                     QASCITVGITGTPGAGKSSLIGELCREFLETVPDKKMAIVAIDPSSSISGGAILGDRT
                     RISIP"
BASE COUNT          524 a          401 c          368 g          620 t
ORIGIN      
        1 gtcattgatg attacaagaa gattcttgga tgcaacagta atggaatgca gatattcctt
       61 ctgttcatgg ttcagttccg tattcgaaag aagatttgaa aatccgatga ttgcattcat
      121 cggagtcctg atttcgtgac tcatatttgc aaggaaaata tccttgaaac ggattgcctg
      181 attcagtctg tcatttgttt ctttaagttt ttcctcatat tctttcttgt ctgtgatttc
      241 atatctgact gcaatgtaat gctgtctgtt tgtctccacg tctctcagcg gaacaattgt
      301 tgcatccacc cagtaatagg ttccatcctt ggctctgttt ttgatctcac ccttccatac
      361 ctttcccgag gtgattgtct cccacatatt tttaaagact tccttggaat ggtatcctga
      421 atttacgatc ctgtgatttt ttccaagaag ttcttctctt gcatatttgg atattctgca
      481 gaaattgtcg tcaacatacg tgataattcc cctgtgatct gttcttgcta caatagccga
      541 ctgttccagt gcggaattca gatcatagat ttcccttgaa tgcctcattt cttcagtaat
      601 gtccctggcg gccgcataaa taagaccggt atccatatca gttgtcgcat gccatgagat
      661 ataaatatat tcaccggata tctttctgta tctgttgatg aaatcagtgc agtcctctcc
      721 ctgattcagt ctattcatta cattcagagt atcattcaca tcatccggat gaacaaaatc
      781 gatgaagggt cttgagagga gctcattttc agaatatcca agtatttttt cgaaagaagg
      841 atttatattt ataaaatatc ccttttcatt cgctatgcag agaagatcca gagaaagttt
      901 aaaaaagttg gaaattgttt tctgtgtttt ttccagtgcc tgatttgctt ttttcagttc
      961 cagaagatgg acaacctgtt ttgccattgc agagagtgca tccttctgac ttggggaaag
     1021 tattttcggc ttactgtcca taacacataa tgttccgata ttgaaaccgt cagtcgtccg
     1081 cagaggcatc cctgcataga atcggattcg gggagaatca actaccaaag gattgttttt
     1141 gaatcgaata tccctctctg catcctctac tgtaagaaga ccgtcatcca taattgcata
     1201 ctggcagaac gaaatgtttc tgggagtttc ctgaatatcc agaccaatct tagatttaaa
     1261 ccactgtctg ttttcgtcaa tcagacagat cagggcaaca ggaacctggc atatttccga
     1321 gatgatcttg gtaatcccgt catactgatc ctcgggcagt gtatccagaa tatcaaatcc
     1381 atatagggtt ttcaatcttc ccgcttcatt ttcaggaact ggatattcag ccataatgct
     1441 agaaatctct cacatacttt cattgtaaac catatcttgc atcctcacta ttgcatttaa
     1501 gtcggcagcg actggaaaat tttccgatta ttattcaggc agaatcatta aaaaagaaat
     1561 tcctgatttg attgaatctt tttttctgtc actatgaacg agatttcaga atggatacag
     1621 aatgcatcag atggggataa gttttccata gcccgtctga tttccttgat tgagaacaag
     1681 ttttcttttg aaaacaggaa aaaagttttt tccatcctcg acagacaggc atcctgcatt
     1741 acagtcggaa ttacgggaac accgggagcg ggaaagtcat ctctgatcgg agaactctgc
     1801 agagaattcc tggaaacagt cccggataaa aaaatggcta ttgtcgcaat tgatccgtcg
     1861 agcagcatca gcggaggtgc catacttggt gacagaacaa ggataagcat tcc
//
LOCUS       DAOQGJ010000770         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_41972_length_1912_cov_2.821756, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000770 DAOQGJ010000000
VERSION     DAOQGJ010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41972_length_1912_cov_2.821756"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PK683_12035"
     CDS             <1..1228
                     /locus_tag="PK683_12035"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH09222.1"
                     /translation="RVIFEFSPLKAFFSFLVLNIFIFFSSVFMGSQMLRYFADRRVCR
                     TPEPVSLKESVYCVSTVILNSAVTLAGWYLWKLDIIKIKSSVDIQSFLDILVLFFSLD
                     FFMYILHRIAHIRFFYPLLHKTHHNYKEVKPVSLFVLNPLENLSFGLLWLCTLTVYPT
                     CWISITAFLTLNLLFGTLGHLGVEPLPDKMKGGLFMKIFTTSSFHSFHHSDPDHNFGF
                     YTMIWDRVFNTGAVYYNEFFGRIPDLKNRMSVLLIIIPLFFQCSSPKKNVMEIRESLI
                     PGAGKGVFALSDIPKNTVIGQYTGKFITEQEHIALAEKNEWHYVMGLLECARENAGGY
                     ALIDGRFGNLTSRINYAPAKFQNVKFMKVCEPPFVNIISLRDIKSGEELYVDYGPAYD
                     YSFMKDPKVRKFFKLP"
     gene            complement(1261..1884)
                     /locus_tag="PK683_12040"
     CDS             complement(1261..1884)
                     /locus_tag="PK683_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH09223.1"
                     /translation="MIIFFIYPTITILLAWAIFKDKPNLSLVFAMISIYIGGFLTVPE
                     SAFSAKGESNMLLGAIAAALSGITFAGYVTTIKLAKMHPAPFSIAVFTIILVLGCVTY
                     PLFPDFMNVDISNWDELLIGCVLLAATTLIGYLLNNYGVPIIGPSLASVISASGPAVT
                     AMMALGIIDEKLNKYQVLGVFLVTLWVLGISLENMRKQKEAAAAAQK"
BASE COUNT          560 a          378 c          377 g          597 t
ORIGIN      
        1 cagggtgatt tttgaatttt cacccttgaa agcatttttc tcctttttag ttctgaatat
       61 ttttatcttc ttttcttcgg tgtttatggg cagtcagatg ctgcgatatt ttgctgacag
      121 aagagtttgc aggactccgg aaccagtctc tttaaaagaa tctgtctact gtgtttcgac
      181 tgtaatttta aactcagcag tcaccctggc aggatggtat ctttggaaac tggatatcat
      241 taaaataaaa tcttctgtgg acattcagtc ttttctcgac atactcgtac ttttcttttc
      301 cttggatttt tttatgtata tccttcatcg gattgcacat atccgatttt tttatcctct
      361 tctccataaa acacatcata attacaaaga ggtaaagccg gtttctcttt ttgttctgaa
      421 tccgctggaa aatctgagct tcggactgct ctggctttgt acccttaccg tatatcctac
      481 ctgctggata agcattaccg cttttctgac tctgaacctt ctgttcggca ctctggggca
      541 tctgggtgtt gagccgctcc ctgataaaat gaaaggaggc ctttttatga aaatttttac
      601 cacaagcagt tttcattcat ttcaccattc cgatccagat cacaatttcg gcttttatac
      661 aatgatctgg gacagagttt ttaatactgg agccgtatat tataatgagt tttttggaag
      721 aatccctgat ttgaaaaatc gaatgtcagt tcttttgatc attatccctc tttttttcca
      781 gtgcagttct cctaaaaaaa atgtgatgga aatccgtgaa tcattgatcc ctggtgcagg
      841 aaaaggagtt ttcgcattat cagatatccc taaaaatact gtaatcggtc agtatacagg
      901 aaaattcatc actgaacagg aacatatagc tcttgctgag aagaatgaat ggcattatgt
      961 aatgggcctt ctggaatgtg cgagagaaaa tgccggagga tatgctctga ttgacggaag
     1021 gtttggaaat ctgaccagtc ggattaatta tgcccctgcc aaatttcaga atgtgaaatt
     1081 tatgaaagtc tgtgaaccgc catttgtaaa tattatatca ttacgggaca ttaaaagcgg
     1141 agaagaactg tacgttgatt acggaccggc ttacgactac agttttatga aagatccgaa
     1201 agtaagaaag ttctttaaac tgccctgagg tcgaagggca gtctttataa aaatttaatt
     1261 ctatttctgt gcagctgcag ccgcttcttt ctgctttctc atattctcaa gactgattcc
     1321 taaaacccag agagtcacca gaaatacgcc tagaacctga tacttattga gtttttcatc
     1381 gattatcccc agtgccatca ttgcagtgac tgcaggaccg ctggcactga tgactgaagc
     1441 aagtgaaggg ccaatgatag ggactccata gttgtttaga agataaccaa tcagagtagt
     1501 cgctgccaga agaacgcatc ctataagcag ttcatcccag ttactgatgt caacattcat
     1561 aaaatcggga aaaagaggat atgtcacaca gccgagtaca agtatgattg tgaatacagc
     1621 gattgaaaac ggtgcaggat gcattttagc gagcttaatc gtagtcacat aaccggcaaa
     1681 tgtaatcccc gaaagagcag ctgcaattgc tcccagaagc atatttgact cacccttggc
     1741 ggagaaagca ctttccggaa cagtcaaaaa gcctccaatg taaatggaaa tcatcgcaaa
     1801 tacaagagaa agattcggct tatccttaaa aattgcccat gccagaagga ttgtaattgt
     1861 cggatatata aaaaatatta tcatattgtg atatccttat tatgtaataa at
//
LOCUS       DAOQGJ010000771         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42090_length_1908_cov_3.957906, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000771 DAOQGJ010000000
VERSION     DAOQGJ010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42090_length_1908_cov_3.957906"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /locus_tag="PK683_12045"
     CDS             complement(<1..1852)
                     /locus_tag="PK683_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH09224.1"
                     /translation="MTKGAAEGQLSVRADASKHSGDFRKIVTGINETLDAVVIPLSKA
                     SEYVEKISKGTIPSRLEENYKGDFRIIQDSLNMCIDSLNGLILSMNDMSNQHEQGDID
                     AAIDSEKFQGAYQDMAKGINKMVSDHIRVKKAAMNVFREFGEGNFESDIEKLPGKKRF
                     INETVDQVKNSLKGLIAEINRVSSEHEKGDIDIAIDAGRFQGDFKSMAEGINEMVGAH
                     IAVKKKAMNVFKEFGEGNFEASMERLPGKKVFINDTIDQVRSNLKLVISEINSLSMSA
                     IDGKLSERADISKHNGDFRKIIGGVNQTLEAVIRPVQEASTVLAELANGNLTETVKGD
                     YRGDHSIIKNSLNKSIEAFSEIIANLAVADEETRSGINTISESSQSLSQGSSEQAASI
                     EEISSTVTELSAQAKQATVNIRQANELSFSSLESSRHGSEMMEKMMNAMQEIKDASDN
                     ISKILASIDEIAFQTNLLALNAAVEAARAGRYGKGFAVVAEEVKSLSGRSANAAKETA
                     GLIQESIQKANKGVEIASKTVKALDEISSSVKNVTSLISEVSKASEEQMTALSEVEKS
                     IQQIEQVIQNNAFSSEETASATLQLSDQAENISRQSEDFRFLLHCLNRKGV"
BASE COUNT          455 a          472 c          331 g          650 t
ORIGIN      
        1 ttactccttt tctgttcaag cagtgcagaa ggaatctgaa atcttccgat tgccggctga
       61 tgttttccgc ctgatccgaa agctgaagcg ttgcggaggc agtctcttca gaactgaatg
      121 cattattctg tatcacctgc tctatctgct gaatactttt ttccacctct gacagagcag
      181 tcatctgttc ttctgatgcc ttggatacct cactaatcag actggtaaca ttttttacac
      241 ttgaactgat ttcatcaagt gccttgactg tttttgaagc aatttccact cctttgttcg
      301 ctttctgaat ggattcctga atcagtcctg cagtctcctt agctgcattt gcacttctgc
      361 ctgacaggct cttcacttct tctgccacga ctgcaaaccc ctttccgtat ctgcctgctc
      421 ttgcagcctc aactgctgca ttgagcgcaa gcagatttgt ctggaatgct atctcatcaa
      481 tggatgcaag tatcttggag atattgtccg atgcatcctt gatttcctgc attgcattca
      541 tcatcttctc catcatctca ctgccgtgtc tggagctttc cagtgaactg aaagacagtt
      601 catttgcctg tcggatattc acagtagcct gtttagcctg agcagaaagt tcagtcactg
      661 tagaagaaat ctcttctatg gaggccgcct gctcgcttga accttgagac aggctctgac
      721 ttgactccga aatggtattg attccggatc tggtttcttc atctgccact gccagattgg
      781 caataatctc gctgaacgct tctatacttt tatttaatga atttttaata atcgaatgat
      841 cgcctcggta atcacccttg actgtctccg tcagatttcc gtttgccaac tcagcaagaa
      901 ctgtagaggc ctcctgaaca ggcctgatca ctgcttccag tgtctggttc acccctccaa
      961 taattttccg gaagtctcca ttgtgttttg aaatatcagc tctttctgag agtttaccat
     1021 cgatcgctga catagaaaga gaattaattt cagaaatgac aagtttcaga tttgatcgga
     1081 cctgatcaat tgtatcatta ataaatactt tttttccggg aagtctttcc atactggctt
     1141 caaaatttcc ttctccgaat tccttaaata cattcattgc ttttttcttt actgcaatat
     1201 gggcaccgac catctcatta atgccttcag ccatactttt aaaatcgccc tgaaatctgc
     1261 cggcatcaat ggctatatcg atgtctcctt tctcatgttc ggaagaaaca cgatttatct
     1321 cagcaatcag tcctttcaaa ctgtttttaa cctgatcaac tgtttcattg atgaatcttt
     1381 tttttccagg aagcttctca atatcagatt caaaatttcc ttctccgaat tctctgaaga
     1441 cattcattgc tgccttcttt accctgatat gatcagacac catcttattg attcccttcg
     1501 ccatatcctg ataagcaccc tgaaattttt ctgaatctat tgctgcatct atgtcccctt
     1561 gctcatgctg atttgacata tcattcatcg aaagtatcag accattcaat gaatcaatgc
     1621 acatattcag actgtcctga atgatgcgga aatcgccttt ataattttcc tccagcctgg
     1681 aaggaattgt gcctttagat atcttttcta cgtattcgga ggctttgctc aatggaatga
     1741 ctactgcatc aagagtctca ttgatgcctg ttactatttt cctaaaatct ccactgtgtt
     1801 ttgaagcatc tgccctgaca gaaagctgtc cctccgcagc tcctttggtc aattcctcag
     1861 tatcactgac taaatgcctc aggctgtctg agattttttt taatgatg
//
LOCUS       DAOQGJ010000772         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42139_length_1907_cov_2.698164, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000772 DAOQGJ010000000
VERSION     DAOQGJ010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42139_length_1907_cov_2.698164"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK683_12050"
     CDS             complement(<1..222)
                     /locus_tag="PK683_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09225.1"
                     /translation="MTYRLIKRDGVSVIAHWWIFILFLLTVLFSSALFANPFDSFEDE
                     LKDYIDSENNQNIDLDPQLKELFQPEDKSE"
     gene            complement(223..618)
                     /locus_tag="PK683_12055"
     CDS             complement(223..618)
                     /locus_tag="PK683_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system-associated lipoprotein"
                     /protein_id="HNH09226.1"
                     /translation="MNRILLFSLLLLAFQECQRKTIKKDDLALLNEEYSAKTYYLKQN
                     FYITHKDILPKGTSVKIWIETSASLIKIKCYPSNQERESAIGKMVAFIIQEELKDKPL
                     TSDRLDSLISEKLEEYSTTPNNSEIIRKK"
     gene            635..1780
                     /locus_tag="PK683_12060"
     CDS             635..1780
                     /locus_tag="PK683_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09227.1"
                     /translation="MTNIRVKPRLACNMGDSEKEEKIISLPLLRKQFRNVYITPSSVR
                     KTEIIQDEPQEIRPEIKQVKEKIFSKELEDLVQKVSQKKKEQSVLDSTPIRVFEKDEE
                     AYFSALHSVFSGIDKGRNLALYCAESFEKIGIRAFLWSTYVTDGGFYKPIFGRGISDV
                     TVENFYLHKRDLFWEEDTESPVFVEFSQSAVENPHFRKKISTTDRNTFSGMLIIPYSN
                     PVSKLIVFCRESEPSGKFEKLSPAVCDVLDTVSPILSEISADEDNLNDQDRFNFFECM
                     QNFAERHIYSGTADRLYIVKITAANYLSLKIRRDLLGRLFQRLYSAADRTDLVAESAK
                     NEFIYISLSDPSARIYSILSEFQDSGLQFLLERRQFPDEGNNFCLYF"
BASE COUNT          543 a          378 c          399 g          587 t
ORIGIN      
        1 ttctgacttg tcctcaggct gaaagagttc tttcagctgc gggtctaaat cgatattctg
       61 attgttctcc gagtcaatat aatcctttaa ttcatcttca aatgaatcaa atggatttgc
      121 gaatagagcc gaagaaaaca gcactgtaag taaaaataaa atgaaaatcc accaatgagc
      181 aatcacggaa acaccatccc tttttattag acgatacgtc aattattttt tacgaataat
      241 ttctgaatta tttggagttg tactgtattc ctccagtttt tcgctgataa gcgaatccag
      301 acgatcactt gtcaaaggtt tgtctttgag ttcttcctga atgataaatg ccaccatttt
      361 tccaattgcg ctctcgcgtt cctggttgct tggataacac tttattttaa tgagagatgc
      421 tgatgtttct atccatatct ttacagaagt acctttcggc agaatgtctt tgtgagtaat
      481 ataaaaattc tgtttcaaat agtaagtttt tgcagagtat tcttcattca gaagagccag
      541 atcgtctttc ttgattgttt ttctctggca ctcctgaaag gcaagaagca gcagagaaaa
      601 tagtaaaatt cggttcatat ttccctaagt acgcttgaca aacatacggg tcaagcctag
      661 attagcctgc aatatgggag attcggaaaa agaagaaaaa atcatttcac tgcctctttt
      721 gagaaaacag ttccgaaatg tctacatcac tccttcttcc gtcagaaaaa ctgaaatcat
      781 acaggatgaa ccccaggaaa tccgtcctga aatcaaacag gtcaaggaaa agattttttc
      841 caaggaactc gaggatctgg ttcagaaggt atcacagaaa aaaaaggagc agtctgttct
      901 cgacagcact ccgatccgcg tattcgaaaa ggatgaggaa gcctattttt ctgcacttca
      961 cagtgttttt tccgggattg acaaaggcag aaatctggca ctctactgtg ctgagagctt
     1021 tgaaaaaatc ggaatcagag catttctatg gagcacttat gtgactgacg gagggtttta
     1081 caagcccatt ttcggacggg gcatcagtga tgttactgta gaaaactttt atctccataa
     1141 aagagattta ttctgggagg aggacacgga aagtccggtt ttcgttgaat tttcccagtc
     1201 tgcggttgaa aaccctcatt tcaggaaaaa aatttctaca actgaccgaa atacattttc
     1261 cgggatgctg atcatccctt attccaatcc tgtttcgaag ctgatcgtgt tctgcagaga
     1321 gtcggaacct tccggaaaat ttgagaagct ttctcccgct gtctgcgatg ttctggacac
     1381 tgtctccccg atcctgagcg agatttctgc tgatgaggac aatttgaacg atcaggacag
     1441 gtttaatttt tttgagtgta tgcagaattt tgcggaaagg catatttact ccgggacggc
     1501 ggaccgtctt tatattgtaa aaatcaccgc agcaaactat ctttcactga aaatcagaag
     1561 ggatctttta ggaaggcttt ttcagaggct ttactctgct gccgaccgga ctgatcttgt
     1621 tgctgaatct gcgaaaaatg aatttatata catttctctt tcggatccgt ccgcaaggat
     1681 ttattccatt ctgtctgaat ttcaggacag cggtctgcag tttctgctgg aaagaaggca
     1741 gtttcccgat gaaggcaata atttctgtct ttatttttag tctctgcttt tctgctgttt
     1801 ctgcgcgaga tgaaagtctt tcggaggcgg tgcgtcttta taagcaggga agctttgcag
     1861 aagcaattcc gctatttgaa aaaaattctg atctgaaagg tcagagc
//
LOCUS       DAOQGJ010000773         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42148_length_1907_cov_2.178726, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000773 DAOQGJ010000000
VERSION     DAOQGJ010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42148_length_1907_cov_2.178726"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1907)
                     /locus_tag="PK683_12065"
     CDS             complement(<1..>1907)
                     /locus_tag="PK683_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09228.1"
                     /translation="LDTESIKRKKKTEIPDSEKWKLITLYLFHILKKKAVLLSESTKN
                     IKPLALIKVKDDSEYSETVYNYIINELYHDESNIKIILEKIQTEEIDVVPTLREVLQQ
                     EYQFDLAVIMKELKRIISGRTLLYSGDLDKEQKEGFAKIQSNFYESIVFIKRLDEGID
                     LPNIYTIAVINDNDSDFKTSVKQIIGRGVRLPKLTREYDDEIDILQSQSEILHIICDR
                     GKNFEKIIDDIKAELDLNEFLFSNNSKHKVKEINRVKKHLLKNKSIPIIKAMDKTRKD
                     VTLEGLMSDISAVVHSYTQHNCSENQEKTKVFLKFAPNSFFTIIDIFSDDFTFLKEME
                     KNGIPSEKFHLDEDDYKEISARIIKKVPHIPDSDKYKNYIKSYIDDLIQREFYFFKSD
                     EADKEIAKRKFKDSFCYFFDNYIHKHYYQPDYKQIHEDQFFDLEDIFKDAEISIHEDA
                     VGSRQKKKMESLKNDRSKLTELVKLGHYFYQYKHSLYDYTKFDSYTEKQLADYLEGLI
                     SRLENSDDCFWVKSERQFSFQYGNQRYFPDFLFHYSGMMNIIETKAEPFSNQKKNALL
                     NKVNEIDGYTGLLIFSDQMDAIEAQNYDVPFESFLGASEQSFRYEKHKSYIVKSIEEH
                     LKFTEFLPFYSV"
BASE COUNT          560 a          367 c          294 g          686 t
ORIGIN      
        1 acagagtaaa aagggagaaa ctctgtaaat tttaaatgtt cttcaatgct ttttacaata
       61 tatgatttat gcttttcata tctgaaagac tgttcagagg ctcccagaaa agattcaaag
      121 ggaacatcat aattctgggc ttcaattgca tccatctgat cagaaaaaat cagaaggcca
      181 gtgtatccgt caatttcgtt gaccttattc agaagtgcat ttttcttctg gttggaaaaa
      241 ggttctgctt ttgtttcgat gatattcatc attccggaat aatgaaataa aaaatcggga
      301 aaatatcttt gattgccata ctgaaagctg aactgtcttt cacttttcac ccagaaacag
      361 tcatccgaat tctcaagtct gctgatcagt ccttcaagat aatcagccag ctgtttttca
      421 gtataagaat caaattttgt ataatcatac aatgaatgtt tgtactgata aaaataatgc
      481 cccagcttga caagctcagt cagttttgac cggtcatttt tcaaggactc catttttttc
      541 ttttgcctgc ttcctactgc atcttcgtga atcgatattt ccgcatcctt gaatatgtct
      601 tccaaatcaa aaaactgatc ttcatggatt tgtttataat ctggctgata ataatgcttg
      661 tgtatgtaat tgtcaaaaaa ataacagaag gaatccttga acttacgttt agcaatttct
      721 ttatcagctt catccgattt aaaaaaataa aactccctct gaatcagatc atcgatgtag
      781 cttttaatat aatttttata tttatcgctg tccggaatat gagggacctt tttgataatt
      841 cttgcagaaa tttccttata atcatcctcg tccagatgaa acttttctga gggaatacca
      901 tttttttcca tttctttcag gaaagtaaaa tcatcagaaa aaatatctat aatggtaaaa
      961 aaagaattcg gagcaaattt aagaaaaacc ttcgtttttt cctgattttc tgaacagtta
     1021 tgctgagtat aagaatggac tactgcactg atgtcagaca tcaatccttc caaagttaca
     1081 tccttcctgg ttttatccat tgccttgata atcgggatac tcttattctt cagaagatgt
     1141 ttctttactc tgttaatctc tttaactttg tgctttgaat tattggaaaa caggaattca
     1201 ttcagatcca gttccgcttt gatgtcatca atgatttttt caaaattctt tccccgatcg
     1261 cagataatgt gcagaatttc agactgagac tgaagaatgt cgatttcatc atcatattct
     1321 cttgtcagtt tcggaagcct gactccgcgt ccgatgatct gctttactga cgtcttaaaa
     1381 tctgaatcat tgtcattgat tactgcaatc gtgtatatat tcggcagatc aattccttca
     1441 tcgagacgtt ttatgaatac aatgctttca tagaagtttg actgaatttt tgcaaatcct
     1501 tctttctgct ccttatccag atcacctgaa tacaaaagtg tccttcctga aattatcctc
     1561 ttcagctctt tcataatcac tgccagatcg aactgatatt cctgctgcag aacctcccgc
     1621 aatgtaggaa caacgtctat ctcttctgtc tgaatcttct ccagaatgat ttttatgttt
     1681 gactcatcat gatagagttc atttataata taattataaa ctgtctccga gtattcagag
     1741 tcatctttta cctttatcag tgccagaggc ttaatattct ttgtactttc agaaagcaga
     1801 actgctttct ttttcagaat atgaaataaa tacagtgtaa tgagtttcca tttttcggaa
     1861 tccggaattt ctgtcttctt tttccttttt atggattctg tatccaa
//
LOCUS       DAOQGJ010000774         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42197_length_1905_cov_3.794595, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000774 DAOQGJ010000000
VERSION     DAOQGJ010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42197_length_1905_cov_3.794595"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1268
                     /locus_tag="PK683_12070"
                     /pseudo
     CDS             1..1268
                     /locus_tag="PK683_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            1335..>1905
                     /locus_tag="PK683_12075"
     CDS             1335..>1905
                     /locus_tag="PK683_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNH09229.1"
                     /translation="MVLMKPKKIVLAYSGGLDTSVILAWLKDTYECEVVAFCADVGQK
                     EELTGLEEKGKKTGASKVYIEDLRLEFARDYIFPAIQGSAVYEMRYLLGTSLARPIIA
                     KAMVDVALKEGADAFSHGATGKGNDQVRFELGFKSLAPSLKIIAPWRIWDFKGRTDLI
                     EYAKKKGIPVPVTAAKPYSMDRNLMHLSFE"
BASE COUNT          503 a          377 c          519 g          506 t
ORIGIN      
        1 gaatgggacg gggagagtcg gcagcagagc ctgtaggttc cgatgaagga gaaatcatgg
       61 tgaaacttgt tccaaagtct gaatggaaga atgcagaaac tcatgaagaa atgatggaat
      121 atatcaagaa ggtgattctc ggaaaagttc cgtccagcta tatcagtatt tcgcagccta
      181 tagaaaacag ggtgaatatg ctgctcgcag gatcaaaggc ggatgttgta attaaaatat
      241 atggcaagga tctgcatgaa ctgaaagaaa ttgcagaaaa gattgcagaa aaactgaaga
      301 ctgttccggg cgcagccgac gttcgggtgc agagagtcct aggacttcct cttctcgaaa
      361 tcagaccgga cagagacaag attgcaaggt acggagtcac tgtcagtgaa atactgacga
      421 cgacccaggc tctgcgagtc ggatctttcg gcggaaaggt ctttgaagga atgagaagat
      481 ttgatctggt ggcaagactg gatgtggacg tatcaagtct gaactatgtg ggggatgttc
      541 ctgtaatgac ggaaaacggt tccactgttc cgctggatct tgtggccgat attactctcg
      601 aagaaggacc tgcagcaatt tacagagaat ctcttcagag aaggatattc gtcgaggcca
      661 acgtcagggg aagagatatg gtgggtttcg tgaatgaggc aatggaaaag acccgagagt
      721 attccagatc tctgaagaaa gatatatccc ttgaatgggg aggacagttt cagaatttta
      781 cccgtgcaag agacaggctg atgcttgttg tgccgatagc actcatcatc atattcggaa
      841 tgctgatggc tgcattcggt gattttcatt atgcgctggg agtgttcatt gtagttcctc
      901 tttctgcaat gggagggatc ttcggtctgt ttttcagaga tctgccgttc agtattcccg
      961 ccggtgtcgg atttatcgtg gcttcaggga ttgccgttct gaacggagtc gtatatgcat
     1021 ctgtgctgaa gcacaaactg gcagagcata tcaacctgag acctgctgtt atactttctg
     1081 cacttgagtc tctgcggccc gttgtgacta ctgaggttgt ggctcttgtc ggtttcattc
     1141 cgatggcagt ttcatccagt gccggttctg aggttcagag acctctggcc acagttgtca
     1201 tgggcggaat tcttgcatcc acggtatttt cgagatttct tctccctatg gtgatggaga
     1261 agcttcttgc ggcagtcaag gagagggaag atcctggcct ttaaagcttg tcagaaaacc
     1321 tcggtacctg ctttctggtt ttgatgaaac ctaagaaaat cgttctcgct tattccggcg
     1381 gtctggatac ttcagtgatt ctggcctggc tgaaagatac ttatgaatgt gaagtcgttg
     1441 ctttctgtgc cgatgtaggg cagaaggaag agctgacagg tctcgaagaa aaaggcaaaa
     1501 agacaggggc ttcaaaagtt tatattgaag acctgagact ggaatttgca cgtgattata
     1561 tctttcctgc gatacagggc tctgcagttt atgaaatgcg ctaccttctc ggaacgtctc
     1621 ttgcaagacc gatcattgca aaggcaatgg tggatgttgc actgaaagaa ggtgcagatg
     1681 ctttctctca cggtgctaca ggaaaaggaa acgatcaggt gcgttttgag cttgggttca
     1741 agtctcttgc accttctctg aagatcatcg ctccctggag aatctgggat ttcaagggaa
     1801 gaaccgatct catcgaatat gcaaagaaaa aaggaatccc tgttcctgtg actgcggcaa
     1861 aaccgtattc catggacaga aatctgatgc acctttcctt tgaag
//
LOCUS       DAOQGJ010000775         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42294_length_1903_cov_2.551407, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000775 DAOQGJ010000000
VERSION     DAOQGJ010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42294_length_1903_cov_2.551407"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PK683_12080"
     CDS             <1..644
                     /locus_tag="PK683_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNH09230.1"
                     /translation="KKGDFIYGSAGMRMGAYAEYICLPENSVLALKPKNISHEEAAAV
                     PFGGMTAMHFLRRGGIEKGQKVLVYGASGSVGTSALQLAKYFGAEVTSVCSGPNAEFV
                     KSLGSDKVIDYTVENFTEKNEKYDLIFDAVGKIKKADCRTVMKEGGKFVTVGGLEVSK
                     ERSEDLDTLRELIESGKLKPVVDRIYPLEEISSAHAYADTGRKKGNVVIRINH"
     gene            complement(651..1769)
                     /locus_tag="PK683_12085"
     CDS             complement(651..1769)
                     /locus_tag="PK683_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin--cholesterol acyltransferase"
                     /protein_id="HNH09231.1"
                     /translation="MKLILFFLSAVIIQCSSSDQKTSADKNLKPVIFVHGYLGSRLVK
                     EDGEKKETVWITPCQGLNFCTSDLSLKNNDGIESEEILKKITAVPMLYELDIYDSWTK
                     RLLKTEGFQAYFFHYDWRKDNSESAAELEKFTEKISKDHNRKVFGIGHSNGGLLTLSV
                     LNRRPELYEKAVFAGSPFRAGIGFTKDLIHGTKIGSNEKILSSCTLQTFESVFNFLSS
                     DSGTDTQEILFDSEGKRIPFNVYNAEFWKSYNLGPYHKGSECADKSLADFQKQLDKAR
                     VFRKSLLAKKIKYPPLLSVMAENRTVIRKIKGKKEKDSWIWDTENPERDKGDGSVLFS
                     AAHPPDGIPFETFISEAEHTALLNDARTAEAVIDFLKK"
BASE COUNT          532 a          372 c          431 g          568 t
ORIGIN      
        1 ttaaaaaggg ggattttatc tacggttccg caggaatgcg tatgggagct tatgcagaat
       61 atatctgcct tcctgaaaac agtgtgcttg cgctgaaacc caaaaacatt tctcacgaag
      121 aagcagctgc tgttcctttc ggaggaatga cggctatgca ttttctgaga agagggggca
      181 tagagaaagg gcagaaagtt cttgtgtacg gagcatccgg ttcagtcgga acttctgctc
      241 ttcagctcgc aaaatatttc ggagcagaag tgacctcagt ctgcagcgga ccgaatgccg
      301 aattcgtgaa aagcctagga tcagataaag taatcgatta tacagtagag aattttactg
      361 aaaagaacga aaaatatgat ctgatttttg atgctgtagg gaagattaaa aaggcagact
      421 gcagaactgt aatgaaggag gggggcaaat ttgtcacagt agggggactg gaagtttcga
      481 aagagaggtc agaagacctg gacacactca gagaactgat cgaatctgga aaactgaaac
      541 ctgttgtaga cagaatttat cctttggaag aaatttcctc agcccacgcg tatgcagaca
      601 cgggccgcaa aaaaggaaat gttgtcatcc ggataaatca ttaaaaacta ttattttttc
      661 aggaaatcta tgactgcttc tgccgttctt gcatcattca ggagggcagt gtgttctgct
      721 tctgagataa aagtctcaaa aggaattccg tccgggggat gggctgcaga aaaaagcacg
      781 cttccgtctc ccttgtccct ttccggattt tctgtatccc aaatccatga atccttctct
      841 ttttttcctt tgatttttct gatcaccgtt ctgttttcag ccattactga cagcagggga
      901 gggtacttaa tttttttcgc aagaagagat ttcctgaaaa ctctggcttt gtcaagctgt
      961 ttctggaaat ctgcgaggct tttgtctgcg cattctgagc ctttatgata gggtcccaga
     1021 ttgtaggatt tccagaattc tgcattgtaa acattgaacg gaattctctt tccttctgag
     1081 tcaaaaagaa tctcctgagt atctgtgccg gaatcggaag acagaaaatt gaaaacagat
     1141 tcaaatgtct gcagagtgca ggaggaaagt attttttcat tgcttccgat ttttgttccg
     1201 tgaatcagat ccttggtgaa cccaatgcct gctctgaaag gcgatcctgc aaaaactgcc
     1261 ttctcgtaaa gttcaggcct gcggttcaga acagacagag tgagaagccc cccgttgctg
     1321 tgtcctattc cgaagacttt cctgttatga tccttggaga ttttttctgt gaatttttcg
     1381 agttcagctg cggattcaga attgtccttc ctccagtcat aatgaaaaaa ataagcctgg
     1441 aatccttctg tttttaaaag tcttttagtc caggaatcat aaatgtcaag ctcgtaaagc
     1501 attggaacag ctgtaatctt tttcaggatc tcttctgatt cgataccgtc attatttttt
     1561 aatgacagat ccgatgtgca gaaattcagt ccctggcagg gagtaatcca gacagtttcc
     1621 tttttttctc cgtcttcttt tacaagtctg cttccaagat atccgtgaac gaatatgact
     1681 ggtttcagat ttttatcagc ggaagttttc tgatctgagg acgagcattg aataataact
     1741 gctgagagga aaaataaaat cagtttcata gttagattct tctgaacact gttttaaatg
     1801 catctttttt ttaagaggtc aaggaatatt agaactgtct tggctgaaag ttgctgggat
     1861 tcagtatgga cgcactgtcg cctacagccc tgatgacgaa aag
//
LOCUS       DAOQGJ010000776         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42379_length_1901_cov_2.692849, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000776 DAOQGJ010000000
VERSION     DAOQGJ010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42379_length_1901_cov_2.692849"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PK683_12090"
     CDS             complement(<1..431)
                     /locus_tag="PK683_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09232.1"
                     /translation="MGKCADLGIPEHLHQNGRKAENDNFTPDERMFRRFRPKDSLSEI
                     VKTNFDFKSMSFNRERYSNPEDVLYNIKENNHYNDCGVASLTVYEIERIQIPHPDDKQ
                     LYTFKAVHDPEECMYPHSIVQVFKNGNKVSEIKPSSVKSLI"
     gene            complement(410..889)
                     /locus_tag="PK683_12095"
     CDS             complement(410..889)
                     /locus_tag="PK683_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09233.1"
                     /translation="MKALFDYDGIKDNRRIFPYEETGNENVSAELFYKKINHTESYNL
                     TEKIWTEYRKSQEESDDDSTPPDRGSFLKSVHFIKKINGYRSFVKSVCVLGDGGFSFR
                     FQANDTEHIDIDIYNGDSTFVFLHSKKGEKTEAFDLSLKDLIIKLKGVLKKWENAQI"
     gene            complement(886..>1901)
                     /locus_tag="PK683_12100"
     CDS             complement(886..>1901)
                     /locus_tag="PK683_12100"
                     /inference="COORDINATES: protein motif:HMM:NF025278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4209 domain-containing protein"
                     /protein_id="HNH09234.1"
                     /translation="FAELAAAKYKEIKYQEEIDSVTEKIIDWKKKLDFKKIETSVPPD
                     DEYEKQTNDLLDNFSKLSGEDLLLALTFSSVLIPESEKMVKIAKSISSERSFSDLIPI
                     EIRDHYGNVAQRFETENEIGNYNNLNIYNIALKYIKLPIIRNIVFTAMYEERLTLNHL
                     IDYFSKYAWFAKPYERNINGTVIEVNWLSLIFPSLKEFFLKVEEMIQTKKGTDFILAV
                     DSLTLKLEGIIRNLCEILGIKVFKEVRDKSDQGKERTIQQQADINMLLKEPKLREQIT
                     ADDISFFEFLLIEKKGYNLRNRVAHSLLFFQEYGFDLMVLLLMALLRLSGYEINNEEG
                     DAI"
BASE COUNT          556 a          384 c          280 g          681 t
ORIGIN      
        1 ctgattaagg atttgactga gcttggtttt atttcggaca ctttatttcc attcttaaag
       61 acctgaacta tagagtgcgg gtacatacat tcttccgggt catggactgc tttgaaggtg
      121 taaagctgct tatcatcagg atgaggaatc tgaattcgtt caatttcata aaccgttaag
      181 gatgccactc cacaatcatt ataatgatta ttctctttta tattgtatag cacatcctca
      241 ggatttgagt atctttcacg attgaaagac atcgatttga agtcaaaatt ggtcttaacg
      301 atttcggaca aggaatcttt tgggcgaaat cgtctgaaca ttctttcatc cggagtaaaa
      361 ttatcatttt cagccttccg gccattttgg tgcaaatgct ccggtattcc taaatctgcg
      421 cattttccca tttcttcaaa actcctttca gcttaattat gagatccttc aatgataaat
      481 cgaacgcctc tgttttttct ccctttttcg aatgcaggaa tacaaacgtg ctgtccccat
      541 tatagatatc aatatctata tgctcagtat catttgcctg aaaacggaaa ctgaaccctc
      601 cgtctcctaa aacacagact gattttacaa atgacctgta cccgttgatt ttctttatga
      661 agtgaacaga cttgagaaaa gagcccctgt ctggaggagt cgaatcatca tcagactctt
      721 cctggctttt tctgtattct gtccatattt tttctgtcag attatatgat tctgtatgat
      781 ttattttttt ataaaacaac tcagcagaaa cattttcatt tcccgtctct tcgtaaggaa
      841 aaatacgcct gttatctttg attccgtcat aatcaaataa tgctttcata ttgcatcccc
      901 ttcttcattg ttgatttcat atccggataa cctcagcagt gccatcaata ataaaaccat
      961 taaatcaaaa ccatattcct gaaaaaatag taaagaatgc gcaacccgat tgcgtaagtt
     1021 ataccctttt ttttcgatca gcagaaactc aaaaaacgaa atatcgtccg cagtaatctg
     1081 ttctcgcagt ttaggttctt tcaatagcat attgatatct gcttgctgtt gaattgttct
     1141 ttctttccct tgatctgatt tatctcgaac ctctttaaaa acttttatac ccagtatttc
     1201 gcaaagattc ctgataatgc cttctaattt aagtgtcaaa gagtctacgg ccagaataaa
     1261 atcggtccct ttttttgtct gaatcatttc ctctactttc aaaaaaaatt ctttcaatga
     1321 aggaaaaatc agagaaagcc aattgacttc tattacggta ccatttatat tccgttcata
     1381 cggctttgca aaccatgcat attttgaaaa atagtcaatc agatgattta aagtcagtct
     1441 ttcctcatac attgcagtga atacgatatt tctaattata ggcaatttta tatattttaa
     1501 tgctatgtta tatatattta aattattata atttcctatc tcattttctg tttcaaacct
     1561 ctgagcaaca tttccgtaat gatcccttat ttcaataggt ataagatcgg aaaaactgcg
     1621 ttctgaactg atggatttcg caatttttac catcttttct gattcaggaa taagcactga
     1681 actaaatgtc aatgccagca gtaaatcctc cccggacagc tttgaaaaat tgtcaagcaa
     1741 atcatttgtc tgcttttcat attcatcatc gggaggaaca gatgtttcaa tctttttaaa
     1801 atcgagcttt ttcttccaat caataatctt ttctgtaaca gaatcaattt cttcctgata
     1861 tttgatttct ttatattttg ccgctgccaa ttctgcaaaa t
//
LOCUS       DAOQGJ010000777         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42460_length_1899_cov_2.419740, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000777 DAOQGJ010000000
VERSION     DAOQGJ010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42460_length_1899_cov_2.419740"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..>1899)
                     /locus_tag="PK683_12105"
     CDS             complement(24..>1899)
                     /locus_tag="PK683_12105"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNH09235.1"
                     /translation="DITVTLTDNDSAGFTVTPISGNTTESGGTATFTVRLNSLPTASV
                     TLPLGSSNTAEGTVSPSSLTFTTANWNTPQTVTVTGADDFIADGTVAYTVILSSAASA
                     DTNYSGLNPPDVSLSNTDNDIAGYTLNTVNSITTDGGQIAASYTMKLNTYPTANVSFN
                     LSSSDTSEGTVSPSSVTFTSANWNTLQTITVNGVSDGINDGNKSLTLIISPATSADPN
                     YNGMDPADKTVQSCDNDGPGTKISFCRASNSFFTTEAGGTAILYMIGSQSPTANAVFS
                     VTSSNTAEGTVSPASITLNSGNWNTFSVSNRVTVTGIDDTLYDLNQNYLINFGSITST
                     DPFYSGYTPLSASLYNQDNDPAFIITAISGNTNESGTTASFTVRLGNRAPTGNVTVNV
                     SSSNTAEGTVSPSVLTFTPANYSTPQTVTVTGADDSLRDGDQSYTIILGAASSIDSAY
                     NGMDPTDVSLQNIDNDKRIFLTAGFFSGALSGAAGADTLCSIDANYPGTGTFKAMIAQ
                     GSVRRASVTANVGDSQSGWVLKPNYHYMTTANIPLFTTGANSLFVFGTLSNALGSGNV
                     WTGLNADWTNSPQHCSNWTSSSSGSSGNIGDASAVDFNLNSSETDTCDTLKHICCVEQ
                     "
BASE COUNT          483 a          403 c          486 g          527 t
ORIGIN      
        1 agattccgcg tgaaaagaaa taactactgt tccacgcagc agatatgctt caaagtatcg
       61 caggtgtccg tttcggaaga gttcagatta aaatcaactg ccgatgcatc acctatattt
      121 ccggatgatc cgctggaaga agaagtccag ttcgagcagt gctgaggaga atttgtccag
      181 tctgcattga gtcccgtcca gacatttcct gatccgagtg catttgaaag agttccgaaa
      241 acgaaaagag aatttgcacc tgttgtaaat agcggaatat ttgcagttgt catataatga
      301 tagttcggtt tcagtaccca tccagactga ctgtctccga catttgcggt cacggaagct
      361 cttcttacag atccctgcgc aatcattgcc ttgaatgtac cggtgccggg ataatttgca
      421 tctatgctgc agagagtatc tgcacctgct gctcctgaaa gtgcaccgct gaaaaacccg
      481 gcagtaagaa atattctttt atcattatca atattctgaa gggatacatc cgtcggatcc
      541 atcccattgt atgcagaatc aatggaagat gctgctccaa ggatgattgt ataactctga
      601 tctccgtcac ggagagaatc atcggctccc gtcacagtga ctgtctgagg tgttgaataa
      661 ttggcaggag taaacgtgag aacggaaggg gaaacagtgc cttcagcagt attgctgctg
      721 ctgacattga cggtcacatt tcctgtcggt gccctgttcc ccagtctcac tgtaaatgaa
      781 gcagttgtcc cggattcatt tgtattgcct gatattgctg ttataatgaa agcaggatca
      841 ttgtcctgat tgtaaagaga agcggaaagg ggagtgtatc ctgaataaaa tggatcagtg
      901 cttgtgattg aaccgaaatt gatcaggtaa ttctgattca gatcatacag agtgtcatca
      961 attccggtga ctgttaccct gttggaaaca ctgaatgtat tccagtttcc gctgttcagg
     1021 gtaatgctcg caggtgaaac cgtgccttca gcagtattgc tgcttgtcac actgaataca
     1081 gcatttgctg ttggtgactg actgccgatc atatagagta ttgcggtgcc gccggcttca
     1141 gtagtgaaaa aagagtttga ggccctgcag aaagaaatct ttgtgcctgg tccgtcattg
     1201 tcacagcttt ggacagtctt gtctgcggga tccatcccgt tgtaattcgg atctgcactt
     1261 gtcgcggggg aaatgatcag ggtcagactc ttgttgccgt cattgattcc gtccgatact
     1321 ccgtttaccg taattgtctg aagagtattc cagttggcag aagtgaatgt aacagaggat
     1381 ggggaaaccg tcccttccga tgtatccgaa ctgctcagat taaaactgac attcgctgtg
     1441 ggatatgtgt tgagcttcat tgtataactt gctgcaatct gtccgccgtc ggtagtgatg
     1501 gaatttaccg tattgagtgt ataacctgca atatcattgt ccgtattgga aagagacaca
     1561 tccggaggat tcagaccgct gtagtttgtg tctgcacttg cagcacttga taaaatgaca
     1621 gtgtaggcga cggtgccgtc agctatgaaa tcatcggcgc ctgtgactgt caccgtctga
     1681 ggagtattcc agttggcagt agtgaatgta agtgaagagg gagataccgt tccctccgct
     1741 gtattgcttg aacctagagg cagtgttacc gatgcggtcg gaagagagtt gagacggacc
     1801 gtgaacgttg ctgtaccgcc ggattcagtt gtattgccgg agatcggagt gacggtaaag
     1861 ccggcagaat cattgtctgt cagagtcacc gtaatgtct
//
LOCUS       DAOQGJ010000778         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42549_length_1897_cov_2.391965, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000778 DAOQGJ010000000
VERSION     DAOQGJ010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42549_length_1897_cov_2.391965"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..1483)
                     /locus_tag="PK683_12110"
     CDS             complement(398..1483)
                     /locus_tag="PK683_12110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH09236.1"
                     /translation="MNARCKSFERKDKNTVHIVGAKEQTITGDFLLIASGSYPFHPSN
                     IPFDKDRIHDSDTILELKRFPRSLCVLGAGVIGCEYATIFAALGIPIFLVDGKDEILP
                     FLDWELSGALVKQMKNDGIDVIFNSGLKEIIPAADDAGDLKVLLTTGETLNVDMFLFA
                     AGRSGRTAGLGLEQAGVKMGKRETVEVDEKYCTNIPNIYAVGDVIGFPALASTSMDQG
                     RAAVTHMFGTEGVEKLASIFPYGIYTVPEISMVGLTEEDARSGGVEVCIGKARYSDMA
                     RGKILGAKEGFLKLIFERKTLVIIGVHIIGFIATELIHYGMTLVENKKTVQELISEVF
                     NYPTLHDLYKYAAYDGLGNLSGHKLKD"
     gene            1552..>1897
                     /locus_tag="PK683_12115"
     CDS             1552..>1897
                     /locus_tag="PK683_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09237.1"
                     /translation="MDYAELEGGLRRMLDRLHVEGTATKDLAVDAFLRENDAAKLLGM
                     LYDQRILAEVAFTGPFKLYQRLGHLDLQQITDMDAGAFVQIFAEQPAVHRFSSKMSET
                     TQRIARIVATEYQ"
BASE COUNT          536 a          474 c          365 g          522 t
ORIGIN      
        1 cccagactgc aactggcgat tccgcagcaa caacgcagga ctccggcaca tactcctcca
       61 tatttctgga ttcggtcaca attcagttcg aacgagccaa tgcagaatcc aacatcggcg
      121 gcataagctg gacagcgatc cagtttcagt agaaaaacat cggactgccg atttacataa
      181 tattttacaa ttatttaatt ataaaatatt atcttgaata cggactgcaa gtgagcctga
      241 agaaaatgca cacagaagcc caagccttca gacagatatg cagcttttct gaaatcctga
      301 actcagactc aactccgccg aatctgcctt tgaatgcaag atcgcgcaga atcactgact
      361 gcaaatttga aaagtaagat taaaaatttc cggctgatca gtcttttaat ttatgtccgc
      421 tcaaatttcc gagcccgtca taagctgcat atttatataa atcatgcaag gtggggtaat
      481 taaaaacttc cgaaataagt tcttgaacag tttttttatt ttccacaaga gtcattccat
      541 agtgaatgag ctctgtagca ataaatccga taatatgcac tcctattata acaagtgtct
      601 ttctttcaaa aatcagtttg agaaaaccct cttttgcacc caggattttt cctctggcca
      661 tatcagaata acgggctttg cctatgcaga cttcaacccc tccggacctg gcatcttcct
      721 cagtcagccc gaccattgat atttccggaa cagtataaat accataggga aaaatggatg
      781 ccaatttttc aacaccctca gtcccgaaca tatgcgttac ggctgctctc ccctgatcca
      841 tactggtgct tgccaaagca gggaatccga ttacatctcc tactgcataa atatttggga
      901 tatttgtgca atatttttca tccacttcga cagtttcccg tttccccatt tttacacctg
      961 cctgttccag tccaaggcct gctgtcctgc cgcttctccc tgcggcaaac agaaacatat
     1021 ccacattcaa tgtttctcct gtagtcagca gaactttcaa atcgcctgca tcatctgcag
     1081 cagggatgat ctcctttaac ccgctgttga aaatgacatc tattccgtca ttcttcatct
     1141 gcttcacaag tgccccggaa agttcccagt ccagaaaagg aagaatctcg tcctttccgt
     1201 cgacaagaaa aatcggtatt ccaagcgctg caaatattgt agcatactcg cacccgatca
     1261 ctcctgcacc cagaacacaa agagagcgtg gaaatcgttt caattccaga attgtatctg
     1321 aatcatgaat ccgatccttg tcaaagggaa tatttgaagg atggaatgga tatgaaccgg
     1381 aagcaatcag aagaaagtcc cctgttatag tctgttcctt tgctccgaca atatgaactg
     1441 tatttttatc tttccgctca aacgatttgc atcgtgcatt cattggcttt ttgcgttaaa
     1501 aaacggaatc acgatgtaag ccttttcgca ccttacccta tacccttgta aatggattac
     1561 gctgaattag aaggcgggct tcgccggatg ttagaccgtc tgcatgtaga aggaaccgcc
     1621 accaaagacc ttgcggtgga tgcctttttg cgtgaaaatg acgccgccaa attgctcggt
     1681 atgttgtatg atcagcggat tttggcggaa gtggcgttca caggcccatt caaactgtac
     1741 cagcgtcttg ggcatttgga tctacaacag attacggata tggatgccgg cgcattcgtc
     1801 caaatttttg ccgaacaacc tgcggtacac cggttttcca gcaaaatgtc tgaaaccacc
     1861 cagcgaattg cccgcattgt ggctacggaa taccaaa
//
LOCUS       DAOQGJ010000779         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42568_length_1896_cov_4.350896, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000779 DAOQGJ010000000
VERSION     DAOQGJ010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42568_length_1896_cov_4.350896"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK683_12120"
     CDS             complement(<1..264)
                     /locus_tag="PK683_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09238.1"
                     /translation="MAYLFFLPFFIFLGILLNPLQMSELPSVHTSWFWDKKEESGTLK
                     DKRTDVNPFKLVSGYRLGDRVYSLSSRQAVSADKEEITEVPFFG"
     gene            complement(264..1520)
                     /locus_tag="PK683_12125"
     CDS             complement(264..1520)
                     /locus_tag="PK683_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNH09239.1"
                     /translation="MSLNIFIFSTGTELTAGKSTDTNSGWLANELLGLGYRTRRFSIL
                     PDSAEEIRDEILRCSETEGENLILMTGGLGPTEDDYTLEVVIGITGKKAVVYEPAYEK
                     LKIIAEGRGIEFQDIFPVSQRQTMYPAGASVIHNEIGIAPGFLVRINDHSRLAAFPGV
                     PREMKAMFTDSFIPLLKREYQPRDIFSFSRIIWGVGESVFQEKFIKNHHNLMAERKVE
                     WGVAAKSGHIKVTFRSEDEYSVEKLKSALEEEYYPRLGDDVFQFIHDFLTKKKYTVSS
                     AESCTGGLFSKLVTDRPGSSGYFIGSIVGYHDDVKMKLLGLKKETLEKFGSVSEEAAV
                     EMSEGVQSLMNTHYSVAITGIAGPTGETQFKKIGLVYIAVKSHKHPAEVHKYQLNVGR
                     DNFREYVSNIALFHLYQRLKKDRTIY"
     gene            complement(1517..>1896)
                     /locus_tag="PK683_12130"
     CDS             complement(1517..>1896)
                     /locus_tag="PK683_12130"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HNH09240.1"
                     /translation="YLQYAHARIRSIFRTAETEGDFESFQSLKKTEERERLMFWCARF
                     PEEVYDAAQSMEPHRLCVYLQNLAKTFSKFYIAKDNKIIEKNDTEKRGLAFLCSVTAM
                     CLKEGLAILGISAPEIMERQVQE"
BASE COUNT          497 a          461 c          372 g          566 t
ORIGIN      
        1 cccaaaaaaa ggaacttcag taatctcctc cttgtctgca gaaacggcct gacgcgatga
       61 aagagaatag actctgtccc caagtctgta cccggacaca agcttgaaag gatttacgtc
      121 ggttcttttg tcctttagag ttcctgattc ttcttttttg tcccagaacc aggaagtatg
      181 cacggatgga agttcagaca tctggagcgg attcagaaga atgcccagaa aaatgaaaaa
      241 tggaagaaaa aagagatatg ccatcagtat atggtcctgt ctttttttag tctttggtac
      301 agatgaaaca gagctatgtt ggaaacatat tccctgaaat tgtcacggcc gacgttcaac
      361 tgatacttat gaacctctgc cggatgctta tgacttttga ctgcaatgta gacgagtcct
      421 attttcttga actgagtttc gcccgtcggt cctgcgattc ctgtgatggc aaccgaataa
      481 tgtgtgttca tcagagactg aaccccctcc gacatttcaa cagcagcttc ttcacttaca
      541 gatccgaact tttcaagagt ttcttttttc agtcccagca gtttcatctt tacatcatca
      601 tggtatccca cgatcgatcc gatgaaatat ccggaggatc caggtctgtc cgtaaccagt
      661 ttggaaaaaa gtcctccggt gcagctctct gcgctggata ctgtatattt ttttttcgtc
      721 agaaaatcat gaataaactg aaatacatcg tctcccagtc tggggtaata ttcctcttca
      781 agggcagatt tcagcttttc aacggaatac tcgtcctctg atctgaatgt caccttgata
      841 tgtcctgatt ttgccgcgac tccccactcc acctttcttt ctgccattaa attatgatga
      901 ttcttaatga atttttcctg aaaaacactt tcccctactc cccagatgat cctggagaag
      961 gagaaaatgt ccctgggctg atactccctc ttcagaagcg ggatgaaaga atctgtaaac
     1021 atagccttca tttccctggg cactccggga aaagctgcaa gtctggaatg gtcattgatc
     1081 cttacaagaa accctggagc aattccgatt tcgttatgaa tgactgaggc gcctgcagga
     1141 tacatagtct gcctctgtga aacaggaaat atatcctgaa actcaattcc gcggccttct
     1201 gcaatgattt tcagcttctc atacgcaggt tcatatacaa ctgccttttt accggttatg
     1261 ccgatgacaa cttccagagt ataatcgtcc tcggtaggcc caagtcctcc tgtcatcaga
     1321 atcaggttct ccccttccgt ctcggagcat ctcagaattt catccctgat ctcttctgca
     1381 ctgtccggaa gaatggaaaa acgtctggtt ctgtatccca gtccgagaag ttcatttgca
     1441 agccatcctg aattcgtatc tgtgctcttt ccggcagtca gttctgttcc tgtggaaaaa
     1501 atgaaaatat tcagactcat tcctgaacct gcctttccat tatttcagga gcagagatac
     1561 ccagtattgc cagaccttcc ttcagacaca ttgcagtgac cgaacagaga aatgcaagtc
     1621 ccctcttctc agtgtcattt ttctcaataa tcttattatc ttttgcaata taaaatttgg
     1681 aaaaggtctt tgccagattc tgaagataca cacagagtct gtgaggttcc attgactgag
     1741 cagcatcgta aacttcttca ggaaatcttg cgcaccagaa catcagtctc tctctctctt
     1801 cagttttttt caggctctga aagctttcaa agtctccctc tgtttccgca gttctgaaga
     1861 tggaacggat tcttgcatga gcgtactgaa gatagt
//
LOCUS       DAOQGJ010000780         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42577_length_1896_cov_3.354699, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000780 DAOQGJ010000000
VERSION     DAOQGJ010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42577_length_1896_cov_3.354699"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PK683_12135"
     CDS             complement(<1..842)
                     /locus_tag="PK683_12135"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNH09241.1"
                     /translation="MKTALFIILIFFSDAAGAETESLPVEVLLSSNNSIYLSILRNIQ
                     SVFSGRLNFQYMQSMNEAELKDFFYRTENRGAKLLIALGNQAALAAKENLKNTPVLYS
                     LVNSPRALGFNSSSNTCGIHIDVPVEQFFSTLKEIRPDAKRVAAFYSGNAGEMLVNEA
                     DYADSHFGILFQKIKIEKNEFSEKLNELKGRADAFYMVPDSVYTQENFEILSRFAKEN
                     RIILMTQIPFIVNVGTAFSITPYYARAGSMIGEMANEIFAGRLECTGGYASAVREFSF
                     NLNR"
     gene            complement(867..>1896)
                     /locus_tag="PK683_12140"
     CDS             complement(867..>1896)
                     /locus_tag="PK683_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09242.1"
                     /translation="DPESTDRFLKLQENFRLQAGPSIAYALTDKLGIGLTAYYMYDSA
                     KTVSSSNLSEVSGNVITLMEQERRRTSGIIPVLGLQYMLNEKLSFGASMKKTMVTGGN
                     ISTRSNVTVAGRTGGPSSITSQAGSQMGAIIVGTDPILTNPNTYKVPEVTETRFGTSF
                     FASKRLMISGDLIHTSGYKLAQSNFTYDPTRRAAVFGDPYSNSLKQMQTLNYAFGIEF
                     YATESIVLRFGHFSNLANTEKISWLNAAAEAAIIQATSNRGAPATSITLPIDGSTGYT
                     LPQKREQHISLKGYTFGIGFETAKNSFSIIVSAQAGRGIATIDRNQLPTVSTYNDVTF
                     YVSGGTKN"
BASE COUNT          505 a          431 c          391 g          569 t
ORIGIN      
        1 tctctgttca gattgaatga aaactcccgc accgcagaag cgtagcctcc agtacattcc
       61 agtctgcctg caaaaatctc atttgccatt tcaccgatca tgcttcctgc ccttgcgtag
      121 tacggagtga tgctgaaagc agttccgaca ttcacaataa acggaatctg agtcatcaga
      181 atgattctgt tctcttttgc aaacctggac agaatttcga aattttcctg agtatacact
      241 gaatccggga ccatataaaa tgcatctgct ctgcctttca gctcattcag cttttcagag
      301 aattcatttt tttcaatttt aatcttctga aacagaattc caaaatgaga atctgcatag
      361 tccgcttcat tgacaagcat ctctcctgca tttccggaat agaaagccgc tacccttttt
      421 gcatcaggac gtatttcctt gagtgtgctg aaaaactgtt ccactggaac atcaatatgt
      481 atcccgcagg tattcgagct ggaattgaat cctaaagccc tgggtgaatt tacgagtgaa
      541 tagagcacag gagtgttttt cagattttcc tttgcagcaa gtgcagcctg atttccaagt
      601 gcgatcaaaa gttttgcccc tctgttctct gttctgtaga aaaaatcctt aagctctgct
      661 tcattcatac tctgcatata ctgaaaattc agcctgccgg aaaaaaccga ctgtatgttt
      721 cggagaatac tcagataaat gctgttgttt gaggacagga gcacctctac aggcaaggat
      781 tcagtttctg cacccgcagc atccgaaaaa aaaatcagaa ttataaaaag tgctgttttc
      841 attaatttat aatgaatata tgcctgtcag ttttttgttc cgccggaaac ataaaaagtc
      901 acatcattgt acgtggaaac agtcggaagc tggtttcggt caatagttgc aatgcctctt
      961 ccagcctgcg ccgatacaat tatgctgaat gagttctttg cagtttcaaa accgattccg
     1021 aatgtatatc ctttgagtga aatatgctgt tcccttttct gaggaagagt gtatccggta
     1081 ctcccatcga tgggaagagt aatggatgtt gccggtgcac cccggttgct cgttgcctga
     1141 atgatggctg cttctgccgc tgcattgagc cagctgattt tttctgtatt ggcaagattg
     1201 gaaaaatgtc cgaagcggag cacaatgctt tcagttgcat aaaactctat tccaaaggca
     1261 taattgagtg tctgcatctg cttaagactg tttgaataag gatcaccgaa aactgctgcc
     1321 cttctagtcg gatcataggt aaaatttgac tgtgcaagct tatacccgga tgtatgaatc
     1381 agatcacctg aaatcataag ccttttgctg gcaaaaaacg atgtaccgaa cctcgtttct
     1441 gttacttcag gcaccttgta tgtgttaggg tttgtaagga tcggatcagt gccgacaatg
     1501 attgccccca tctgactgcc tgcctgagat gtgatggaag aaggcccccc tgttcttcct
     1561 gccactgtca cattgcttct cgtagaaata tttccgcctg taaccattgt ctttttcatt
     1621 gaggcaccga aggaaagttt ttcattcagc atatactgaa gcccgagcac tggaataatt
     1681 ccgctcgtcc ttcttctttc ctgctccatc agagtgatta catttcctga aacctcgctc
     1741 agattggagc ttgagacggt ttttgcactg tcatacatat aataagcggt cagacctatt
     1801 ccgagtttat cagtcagtgc gtatgcaatg ctagggccag cctgaagacg aaagttttcc
     1861 tgaagcttta aaaaacgatc cgtggattca ggatcc
//
LOCUS       DAOQGJ010000781         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42596_length_1896_cov_2.178707, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000781 DAOQGJ010000000
VERSION     DAOQGJ010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42596_length_1896_cov_2.178707"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PK683_12145"
     CDS             complement(<1..402)
                     /locus_tag="PK683_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09243.1"
                     /translation="MESESGVFETKVRRNFLDFFRKKLRIDPLNIQYGNQSLRCAEIS
                     ALRYGIIQMYINGIKANRMYEIGLKSQDEKKKIRIFFQSLRPFVTNKKTEEHYNLIIE
                     NLWMHIGSRLAKKAITDLENGKNYQIENLEIT"
     gene            complement(407..1048)
                     /locus_tag="PK683_12150"
     CDS             complement(407..1048)
                     /locus_tag="PK683_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09244.1"
                     /translation="MKNIHFIIITLILSFCGEAKDKIQGAYNKTVAGVEVTDENVVKY
                     VAAVKALREIGPDIPKKLAEREEKIKAGMEIYSQIEETVKKAGFKDAPEFMKLNAKIA
                     WAWNVSQGETGMHKMETMKDEGLAKMDAAINNPATPESAKEELKKAREVINQNWEKNR
                     KYAEMTLKFIRPFTNEKDLEVIKKHQKELMEAYSGLSLKSLNEINASQFLNKE"
     gene            complement(1245..>1896)
                     /locus_tag="PK683_12155"
     CDS             complement(1245..>1896)
                     /locus_tag="PK683_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH09245.1"
                     /translation="TLGVPLNGYEVCLKDDKENKIPYEAGKKGTLWVKSRQILMGYYK
                     RPELNSTVFDKEGFFNTGDLISFTWKGELVFSGRSKDTLVLAGGENVEPVPVEDKLLN
                     SDLIDQVMVVGNDRKSLGAIIVPNFEKVKSAIPHAPADLSLWNSDPKIRDLFKKEIIR
                     LITRDNGFKTFEIIPGNCFCISPRQFDLDTEMTRTLKMKRNVISESFASEIENMYK"
BASE COUNT          443 a          430 c          339 g          684 t
ORIGIN      
        1 cgtgatttcc agattttcaa tctgataatt ttttccattt tcgagatcag tgatcgcctt
       61 ttttgccagc ctgcttccta tatgcatcca tagattttcg ataatgagat tataatgctc
      121 ttctgttttt ttattcgtga caaaagggcg gagggactga aagaagattc ttattttttt
      181 cttttcatcc tgagatttca gacctatttc atacattctg tttgctttga tcccgttgat
      241 atacatctga atgattccat atctcagagc tgaaatttca gcgcatctca gcgactgatt
      301 cccatactgt atgtttaatg gatcaattct cagttttttt ctgaaaaaat ccagaaaatt
      361 tcttctgacc ttcgtttcaa aaactccgct ttccgattcc ataagtttat tctttgttca
      421 gaaactgcga agcattgatt tcgttcaggc ttttcagaga gagtcccgag taggcttcca
      481 tcagttcctt ctggtgtttt tttatgacct ccagatcctt ttcatttgta aaagggcgga
      541 tgaattttaa agtcatctct gcatatttcc tgtttttttc ccagttctga ttgatgacct
      601 ccctggcttt tttcagttct tcctttgcag attcgggagt tgcagggtta ttgatagcag
      661 catccatttt agccagtccc tcgtctttca ttgtctccat tttatgcatt cctgtttcgc
      721 cctgagacac attccatgcc caggcaattt ttgcattgag cttcatgaat tcaggagcat
      781 ctttgaaacc tgcttttttt acagtctctt ctatctgact gtagatttcc attcctgcct
      841 tgattttttc ttctctttca gccagttttt tgggaatatc cgggccgatt tctctcaatg
      901 ccttgactgc agcgacatat tttaccacat tttcatctgt gacttcgact cctgctacag
      961 tcttattgta tgccccctgg atcttgtcct ttgcttctcc gcagaacgac agaatcagtg
     1021 ttataatgat aaagtgaata tttttcatgc aatctccttt cagtatgcga gtgagaaact
     1081 gcggaccgtc agctgtctga tgcgtctgag caatctctct ttacaagtct tttttaaaaa
     1141 cccctgcgtt caacgaaaaa tattcagctg accttgacaa aaaactgcat aatatccgga
     1201 atctgttttg ctgtgaaaca cttgagaata ctgatgcatc cgcactactt gtacatattt
     1261 tcgatctcag atgcaaaact ttccgagatg acatttcttt tcattttcag tgtccttgtc
     1321 atttcagtgt ccaggtcaaa ctgtctgggc gaaatacaga agcagtttcc gggaatgatt
     1381 tcaaacgtct tgaatccgtt gtctcttgta atcagtctga tgatttcttt tttaaacaga
     1441 tctcttattt tgggatctga gttccagaga ctgaggtctg caggcgcgtg cgggattgct
     1501 gatttgactt tttcaaagtt cggaacaatg attgcaccta gggatttccg gtcattgccg
     1561 accaccatca cctgatcaat cagatcggaa ttcaggagct tgtcttctac gggtacaggc
     1621 tctacatttt cacctcctgc aagaacaagt gtatccttgg aacgtccgga aaaaaccagt
     1681 tctcccttcc aggtaaaact gatcagatct cccgtattga aaaatccttc cttgtcaaat
     1741 acagtgctgt tcagttccgg tcttttataa taacccatca ggatctgtct gctttttacc
     1801 cagagagttc ctttttttcc ggcttcataa ggaattttat tttctttatc atccttcaga
     1861 cagacctcgt atccgttcag aggaactccc agtgtg
//
LOCUS       DAOQGJ010000782         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42598_length_1896_cov_1.933188, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000782 DAOQGJ010000000
VERSION     DAOQGJ010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42598_length_1896_cov_1.933188"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK683_12160"
     CDS             complement(<1..195)
                     /locus_tag="PK683_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09246.1"
                     /translation="MEKEEITALKRKSVYPNVGRLICNLIILIILYYSGVYNIFFYSI
                     VFIIIFSAGWTAVEAVFILDY"
     gene            374..862
                     /locus_tag="PK683_12165"
     CDS             374..862
                     /locus_tag="PK683_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP family protein"
                     /protein_id="HNH09247.1"
                     /translation="MKTILLLLSFPMLGILQANDTFAEDLISSHNSFRKHHSASDLKW
                     NVEMQKFAQKWADTIAKKDKMEHRPNNKYGENIYWTSGGKLDAKSAVKAWYDEIKYYN
                     YKKPGFGMKTGHFTQVVWKGSSELGCGSAKSKKGGTYLVCNYNPPGNYEGEFKENVLQ
                     KK"
     gene            complement(913..>1896)
                     /locus_tag="PK683_12170"
     CDS             complement(913..>1896)
                     /locus_tag="PK683_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNH09248.1"
                     /translation="AVSLELIFDFSKTHTYMFLGKTYSVQPNLLVSIPKEGLLGSFTF
                     PDFSMLLTLTFLIQVIAITLVGSVESLLTAAAVDKLDPHKRHSDMDRELLAKGAGNSI
                     LGLIGGLPIIAEVVRSSANINAGAKTRWSNFFHGAFLFAFVLFLPDLIRIIPLAALAS
                     MLIVTGYRLSVPEFVKSLNVGKEQILYFMITVIFSVAEDLLVGIALGMVSKVITTYIF
                     ASKGGADLKSVFSKASSVSNTGQDSAELHLEQVCTFLNYLGLRNNLKSLAPGKNVKVI
                     FSDVKFIDHTTMENLTEFQHEYNSSGGKMEFLGMERLKPFSEHSTAARVFA"
BASE COUNT          630 a          392 c          363 g          511 t
ORIGIN      
        1 ataatccagg atgaaaacgg cttcgacggc tgtccatcct gcagaaaaga taattataaa
       61 aactatactg taaaaaaaaa tattataaac acctgaatag tataaaataa ttaaaatgat
      121 gagattacag atcagccttc cgacattcgg atatacagat tttcttttta atgctgtaat
      181 ttcttctttt tccaattcag tctaccctgc agaattttct tttcaaggaa tacgagaaat
      241 ccggctccct ggacagctca ccttaatgta tactgaactc ggcggagtca gtcaatacag
      301 aatttttttt tgaaagtatg catacaatag tctattctga cagtctaaga aaaaaaccta
      361 aataaggccg actatgaaaa caattctcct gttactttcc ttcccgatgc ttggaattct
      421 tcaggcaaac gatacctttg ccgaagatct tatttcttcc cataattctt tcagaaaaca
      481 ccattccgcc tcagatctca aatggaatgt cgaaatgcag aagtttgcac aaaaatgggc
      541 agacactatc gccaaaaagg ataaaatgga acatcggcct aacaataagt acggtgaaaa
      601 tatttactgg acttccggcg gaaaactgga tgcaaaatct gcagtaaaag cctggtatga
      661 tgagattaaa tactacaact acaaaaagcc tggattcgga atgaaaacag gacattttac
      721 tcaggttgta tggaagggtt cttctgaact gggatgcgga tctgcaaaat caaaaaaggg
      781 cggaacttat ctggtgtgca attataatcc tcctggaaat tatgagggag aattcaagga
      841 aaacgttctg cagaaaaaat agatggaatt gcagggaaca aatcaaagtt ccctgccagt
      901 cttaatttac gtttaagcaa acactcttgc agcagtagaa tgttctgaaa acggtttcag
      961 tctttccatt cccaggaatt ccatctttcc gccggaagaa ttatattcgt gctgaaactc
     1021 agtgaggttc tccatagtcg tatggtcaat aaatttcaca tccgaaaaga taactttcac
     1081 attttttcct ggtgccagac ttttcagatt gtttctgagt cccagataat tcaggaaagt
     1141 acagacctgt tccagatgaa gctctgcaga gtcctgacct gtatttgata cagaagatgc
     1201 tttagagaaa acactcttaa gatcagctcc gcctttggaa gcaaatatat aagttgtaat
     1261 taccttggaa accataccca gtgcaattcc tacaagaagg tcttccgcaa cagaaaaaat
     1321 tacagtaatc ataaaataca gaatctgctc ttttcctaca ttgagagatt ttacaaattc
     1381 cggcacagaa agcctgtatc ctgtcacaat gagcatcgaa gcaagtgctg caagaggtat
     1441 gatgcggata agatcgggca gaaacagaac aaatgcaaaa agaaatgctc catggaagaa
     1501 atttgaccat ctggtctttg ctcccgcatt tatgttggca gaacttctca ccacctcagc
     1561 gatgatagga agtcctccaa tcagcccaag tatcgaattg cctgctccct ttgcaaggag
     1621 ctctctgtcc atatcggaat gcctcttatg cggatccagt ttatccactg cagcagctgt
     1681 aagcagactt tccacacttc ccacaagagt aatagcaatc acctgtatca ggaatgtcag
     1741 agtcagaagc attgaaaaat caggaaaggt aaaacttccc agaagccctt ccttgggaat
     1801 ggatacgagg agattcggct gaacggaata agtctttcct aaaaacatat atgtgtgtgt
     1861 cttactgaaa tcaaagatca gttcaaggga tacggc
//
LOCUS       DAOQGJ010000783         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42648_length_1894_cov_3.227297, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000783 DAOQGJ010000000
VERSION     DAOQGJ010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42648_length_1894_cov_3.227297"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PK683_12175"
     CDS             <1..674
                     /locus_tag="PK683_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09249.1"
                     /translation="GDGNQVLIVDENGNPAGSSQADGNFLSDICFAPDGHTLLVFSGG
                     QNYLLDNSGKIIWKAAEEKDAGYRFYKNGAISEKAERFALHFNEEKNGERKDRVAVYA
                     KTGEPVFELVLDKIYPFKISMAVSDSGQLLVNTNDRVLLFDPKGIPVHRTVKSKEEAV
                     YQTAFETNGTFIASFQNMLLFLNRKGEVYFQKNSPEPVRIFKNRDPSVIFLESPSHLE
                     SMTFY"
     gene            761..1399
                     /locus_tag="PK683_12180"
     CDS             761..1399
                     /locus_tag="PK683_12180"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNH09250.1"
                     /translation="MNLPYLCRMTGKCCTGKIVSLTPFDIFRIAREQDKNPSVLFQEK
                     TLAYRIDLGTFWMVPVIALRKNAECRFLADDRSCNIYSARPFACRLFPLKFDPSLKNF
                     YRSTGAEAVCIECISDGHSVEEEKYLSDSGAGKMQEEYLLTNELISRISLSGFNIQEI
                     KKKKKKQKIFFALQSLMYETYPQNNEESYPFEKMKKEAEKMMSEYGGEDELF"
     gene            1386..>1894
                     /locus_tag="PK683_12185"
     CDS             1386..>1894
                     /locus_tag="PK683_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09251.1"
                     /translation="MNCFSFPGICSSRSLLKNSLPAAEVFLYYALLFLLILQSSCIFG
                     QIPMPLQRHIEKVHTDQKIMIPYLFYMPKEYFSDRYEKRPLVLFLHGKGERGKDLNLL
                     KRQGLPKMIDSGKDFPFFMISPQITEDRNEWPAEIIHDIVMKIADEYPVDRNRIYVTG
                     ISMGGFGTW"
BASE COUNT          575 a          359 c          426 g          534 t
ORIGIN      
        1 caggagacgg aaatcaggtg cttattgtgg atgaaaacgg aaatccggca ggttcatctc
       61 aggcagacgg caattttctt tccgatatct gttttgctcc tgacgggcac acccttcttg
      121 ttttttcagg aggtcagaat taccttctgg acaattccgg aaaaattatc tggaaagcag
      181 cagaggaaaa ggatgcaggg tacagattct ataagaacgg cgccatctca gaaaaagcag
      241 aacgatttgc tctccatttc aatgaagaaa aaaacgggga aagaaaggac agagtggcgg
      301 tttatgcaaa aacaggagaa cctgtttttg aactcgtttt ggataagatt tatcctttta
      361 agatttcgat ggctgtttcg gactctgggc aacttctcgt caacaccaat gacagggtgc
      421 ttctctttga tcccaagggc attcctgttc acaggacagt caaatccaaa gaagaggcag
      481 tttatcagac agcatttgag acaaacggca cttttatagc ctcatttcag aatatgcttc
      541 ttttcctgaa cagaaaaggc gaggtttatt ttcagaagaa cagtcctgaa ccggtcagaa
      601 tcttcaagaa cagagatcct tcagtcatct ttctggaatc gccctctcat ctggaatcga
      661 tgacatttta ctgatcttgg attaaagtaa atgagatata ctgtcaaaat acatacaatg
      721 gatgcagtgc aagtacagag aagtatgtca ttctgctgag atgaatcttc cgtatttatg
      781 cagaatgaca ggaaaatgct gtaccggcaa aattgtaagc ctgacaccat tcgatatatt
      841 ccgtattgca agggagcagg ataagaatcc ttctgttctg tttcaggaaa agactcttgc
      901 ttaccgaatc gatctgggaa ctttttggat ggttccagtc attgctctca gaaaaaatgc
      961 agaatgccgt ttccttgcgg atgacagatc ctgcaacatt tacagtgcaa ggccttttgc
     1021 ctgccgtctt tttcctctca agtttgatcc gtcactgaag aatttttaca gaagcacagg
     1081 agcagaagca gtctgcatag aatgcatctc agacggtcat tcagtggagg aggagaaata
     1141 tctgtctgat tcaggtgcgg gaaagatgca ggaagagtat ctgcttacga atgaactgat
     1201 cagtcggatc tccctgtccg gtttcaatat acaggaaata aaaaagaaga aaaaaaagca
     1261 gaaaatcttt tttgctcttc agtctcttat gtacgagacc tatcctcaaa acaatgagga
     1321 atcatatcct tttgaaaaga tgaaaaagga ggcggagaaa atgatgtctg agtacggggg
     1381 tgaagatgaa ctgttttagt tttcccggaa tctgttcttc tcggtctctt ctgaaaaatt
     1441 ctcttccggc tgcggaagtc tttctgtatt atgctctcct gtttttgctt attctgcagt
     1501 cttcctgcat attcggacag atccctatgc cgcttcagag acatattgaa aaagttcata
     1561 ctgatcaaaa aataatgatt ccatatcttt tttacatgcc gaaggaatat ttttcagaca
     1621 gatatgagaa aagacctctg gttctttttc tgcacggcaa gggggaaaga ggaaaggatc
     1681 tgaatcttct gaaaagacag ggacttccta aaatgatcga ttccggaaag gattttcctt
     1741 tttttatgat atccccgcag atcactgaag acagaaatga atggcctgca gaaataattc
     1801 atgatattgt aatgaaaatt gcggatgaat atccggtgga cagaaacagg atttatgtaa
     1861 cggggataag tatgggagga ttcggaactt ggga
//
LOCUS       DAOQGJ010000784         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42711_length_1893_cov_2.249184, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000784 DAOQGJ010000000
VERSION     DAOQGJ010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42711_length_1893_cov_2.249184"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PK683_12190"
     CDS             complement(<1..327)
                     /locus_tag="PK683_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09252.1"
                     /translation="MVRGRSKIVELDNSRRYVYHRKDEKDKITYCLENVGPSTKDEFY
                     KLMLTQPNGQKANVDWGTNASNIYNVSDIMQFGSTMLFRLCENYINGFYDDYGKDKSR
                     YYREDFK"
     gene            complement(996..>1893)
                     /locus_tag="PK683_12195"
     CDS             complement(996..>1893)
                     /locus_tag="PK683_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metalloenzyme"
                     /protein_id="HNH09253.1"
                     /translation="EKNPFSRFAKGFFLPLGGKELPDSSPYSKGKYVVTDANMGLKGL
                     PQSATGQTSLWTGINAVEVIGRHVSGVPSFTLKKIIAKHSIMKIFKEHGYSSAFLNCY
                     SDVFFSRMEENRRLISASTLIQMAADIPFRTFEDLRKGNGLFMDITHDILQEFGKKFL
                     SPDDELFQKRDPFEMGKRSVVLARNQDLTMFEYFLTDKTGHSQNWQHAEQVIGTVEKF
                     MDGVLSELNPETEHLIVTSDHGNLEDLSDKVHTSNPVPTFLYGKYTDSMKDKIKTLAD
                     IVPAIYGELNIPEKPVYVKQEG"
BASE COUNT          509 a          365 c          371 g          648 t
ORIGIN      
        1 cttaaaatcc tcacggtaat atctgctttt atcctttccg taatcatcat aaaagccatt
       61 gatatagttt tcacagagac ggaaaagcat tgtgcttccg aattgcataa tatcagatac
      121 attgtaaata ttggatgcat ttgtacccca gtcaacgttt gccttctgac cattcggctg
      181 agtgagcata agcttataaa actcatcttt cgtggaagga ccgacatttt ccagacaata
      241 ggttatttta tctttttcat cttttcggtg atatacatat cttctggaat tgtccagttc
      301 tacaattttt gatctgcctc taaccatcgt tgaatagaat gaagaatccg gcagaatacc
      361 atcacattgg ctgaggatta aaagaatgaa tatcagggaa aatatgcttt ttgctttcgc
      421 aaagataaca gataaaagct ttgaattttt aaacttatga agatctccaa agattggagg
      481 tttaaatctg aactgaactt gttcgctgtt cgctgttcgc tgttcgctgt tcgctgttcg
      541 ctgttcgctg tcatccattc caattgaaac agatgctgga aaatttcaat cattattttt
      601 aacttttact tactaaataa taatataatt aaagtatatt atactataaa atcgttattt
      661 ttaccgaact tttttctgct gtcttttctg taaataccca acattgggta ttttttgcag
      721 ttttcttggg aaaaaatagg tgattatcct tattttttca tcggttttag gattcattta
      781 tggatgagtt tacaaaaaaa taagaatagg tattcgattg cagttgttta aaaaagtgaa
      841 atttttttag cagaggagtt tgtttttctg agattgatgg gttttttaag gaagaaagtt
      901 ctctcgtggc gagctggagc tttttaaagg agcagttcat ttgatgggca tcgtatcaat
      961 tccccgccgg ctattcccga ttccccgggg tttaatcacc cttcctgttt cacatacaca
     1021 ggtttttccg ggatattaag ttcaccatag attgcaggaa caatatccgc aagagtcttg
     1081 attttgtcct tcattgagtc tgtatattta ccgtaaagga aagtagggac tggattggat
     1141 gtatgcacct tgtctgaaag gtcttcgagg ttgccgtgat ctgaggtgac gatcagatgt
     1201 tctgtctccg gattcagttc agaaagaacc ccatccataa atttttccac tgtgccgatc
     1261 acctgttctg cgtgttgcca gttctgagaa tgtcctgtct tgtctgtaag gaagtattca
     1321 aacatagtca gatcctggtt tcttgccaga accactgatc tttttcccat ttcaaaaggg
     1381 tctcttttct gaaacagttc atcgtccggg gacagaaatt tttttccaaa ttcctgaagg
     1441 atatcatggg tgatatccat aaaaaggccg tttcctttgc ggaggtcctc aaatgtcctg
     1501 aacgggatgt ctgcagccat ctgaatcagt gtagatgcag atatcagtct tctgttttct
     1561 tccatccggc tgaaaaacac atcactgtag cagttcagga aggcggaact gtatccgtgc
     1621 tccttgaaga ttttcattat ggaatgtttt gcgataattt tttttaatgt aaaactgggc
     1681 acaccgctca catgccttcc tatgacttcg actgcattga ttcctgtcca gagggatgtc
     1741 tgtcctgtag cactctgcgg aagtcctttc aggcccatat tggcatctgt aaccacatat
     1801 ttccctttgg aatacggact ggaatcaggg agttcttttc ctccaagagg aagaaaaaat
     1861 ccttttgcaa accttgaaaa gggattcttt tca
//
LOCUS       DAOQGJ010000785         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42738_length_1892_cov_2.344584, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000785 DAOQGJ010000000
VERSION     DAOQGJ010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42738_length_1892_cov_2.344584"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1289)
                     /locus_tag="PK683_12200"
     CDS             complement(138..1289)
                     /locus_tag="PK683_12200"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNH09254.1"
                     /translation="MGKHKNINSVHTAVFVTVLCLSFAGCLKRNPQKITGLISASGNS
                     TSVQTSTSTSTSDSDSCASSAPDNPANLTVTSSSSPASVSMSWTNPSDCRLKGVLIKR
                     KTGSSPSNVNDGISVDANSDNAGFTDSSVSTGNTYYYKIFLYNGKDQYSSGTGAAVRP
                     GSTTLIPSKIADSSIILDGLDSETAWSGVPKIDFSFPVVPTFTDYTGGNDLNVTGYIK
                     FAYDTNYFYIYYHTDDKYLRVDNAGTPWTDDSIEVFFDMGFNRTSVPDTNDYHLIMTP
                     RSGASYENYGKGTGTVWGVWSPSIVKASNTAGSTLNNDADTDSGWNLEVQIPFTDLGI
                     SSISPGQVIGFTFWVNDDDLSAFTGTQHYFGWTTGTLSANPSTWGILQF"
     gene            complement(1461..>1892)
                     /locus_tag="PK683_12205"
     CDS             complement(1461..>1892)
                     /locus_tag="PK683_12205"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNH09255.1"
                     /translation="MASRLFQKIREDKGLCYSIQSFSSTYSDCGVFTVSCGTSAEKYE
                     KSVNSIMNEIRLLKKKGISKEELEFAKSNQKGSMAIGHELPENKMMDIAVQEMFFGKY
                     YTMQDKYKIIENITLEELNEFVHRIFSVNRMHLSSIGPVSD"
BASE COUNT          520 a          379 c          392 g          601 t
ORIGIN      
        1 atagtatttt taatattctt gattaaaaca agatagattt tatttaatta tttatttaat
       61 aattaatatt tataaaaaca aaagcattat ttaaaaagaa aaaattaaaa ttgtctttat
      121 aaatgaaaaa tatgtaatta aaactgcaat ataccccatg tgctcggatt tgcagaaagt
      181 gtgccggttg tccagccaaa atagtgctgc gttccggtga atgcggaaag atcatcatca
      241 ttcacccaga aggtaaagcc gatgacctga ccaggcgata tggatgagat ccctagatca
      301 gtaaaaggga tctgcacttc cagattccat ccggaatcag tgtctgcgtc attgttcaat
      361 gtgcttccgg ctgtgttact ggccttgaca atggaaggac tccatacacc ccagacggtt
      421 cctgttcctt ttccatagtt ttcatacgag gcaccgcttc tcggtgtcat aatcagatga
      481 taatcatttg tatcaggcac tgaggttctg ttgaatccca tatcaaaaaa tacttcaatg
      541 ctgtcgtcag tccaaggtgt tccggcattg tctacccgaa gatatttatc atctgtatga
      601 taataaatat aaaaatagtt cgtgtcatat gcaaatttta tgtatccggt cacattcaga
      661 tcatttcctc cggtgtaatc tgtaaaagtc ggaaccactg ggaaggaaaa gtcgattttc
      721 ggcactccag accaggccgt ttctgaatcc agtccgtcca gtatgattga actgtctgca
      781 attttggacg gtatcagtgt tgtactgccg ggacggacgg cagctcccgt tccggaagag
      841 tactgatctt ttccgttgta aaggaaaatt ttgtaataat aagtatttcc tgtactgact
      901 gatgaatccg taaatcctgc attgtctgaa tttgcatcta ccgaaattcc gtcattgacg
      961 tttgagggac tgcttccggt ctttcttttt atcagaaccc ctttcagtct gcagtctgac
     1021 ggatttgtcc agctcatact gaccgatgct ggcgaggagg aacttgtgac agtaagattg
     1081 gcgggattgt ccggggctga actggcgcag gagtctgaat ctgaggtgga agtggacgtt
     1141 gaagtctgaa cagaagtcga atttcctgaa gcggaaatca gccctgtaat tttttgggga
     1201 tttcgtttca gacagcctgc aaagcttaag cataagacag tcacaaagac tgctgtatgg
     1261 acactgttta tatttttatg ctttcccatc tctctattat tatctgacga ttataaaccg
     1321 ggggattctc atttttcaga aatatattca ttccatctgg ttcaggggat ggtgcttcag
     1381 aaactgcagg atagacccca aatgagtgaa atcctgcatc agtgtttcgg ctaaagactc
     1441 agatccagat ccttggagac tcagtcagaa acaggaccga tcgatgaaag atgcatcctg
     1501 ttcactgaaa aaatcctatg cacaaattca ttcagttctt cgagtgtgat attttcaatg
     1561 attttatatt tatcctgcat tgtgtagtat tttccgaaga acatttcctg aactgctatg
     1621 tccatcatct tgttttcagg aagttcatgt ccgatagcca tcgatccctt ctggttgctt
     1681 tttgcaaact cgagttcttc ctttgatatc cctttctttt tcagtaaacg tatttcattc
     1741 ataatggaat ttactgattt ttcgtatttt tctgcagatg ttccgcagga gacagtgaat
     1801 actccgcagt cggaataggt ggaggaaaaa ctctgaatgc tgtagcagag tcccttgtct
     1861 tctctgattt tctggaaaag tctcgatgcc at
//
LOCUS       DAOQGJ010000786         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42758_length_1891_cov_3.423203, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000786 DAOQGJ010000000
VERSION     DAOQGJ010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42758_length_1891_cov_3.423203"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1082
                     /locus_tag="PK683_12210"
     CDS             66..1082
                     /locus_tag="PK683_12210"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNH09256.1"
                     /translation="MKKILITGVAGFIGSNVCRHLLGKGYHVIGIDNLSQSDGKNMNE
                     FLNHSEFEFYRVDIMNESSVLRAAHGCSGIIHMAAYKIPRYSDALDTLKINGIGSENL
                     VKAAIENNARLVAASTSDVYGMNPELPFKEDSSNLVMGGPKVRRWAYAISKMFEEQMI
                     YAYGERMNLDFALIRFFGGYGPGQNLTWWGGPQSVFINKALDDEEIEVHGDGQQTRSF
                     THVSDHVDGVTRCLEVEKARGEVFNIGNDREITIENLAKLIWKMIRPNDPPKIKMIPY
                     ETFGKYQDVMRRIPDLTKSKSILGFEPKVKMEDGLKTAIEWQIKRRKELGMTDPKSGC
                     VFKV"
     gene            1079..1828
                     /locus_tag="PK683_12215"
     CDS             1079..1828
                     /locus_tag="PK683_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH09257.1"
                     /translation="MMKKIYIITPVLNEEPNILRLLSGWKKVKSDLKEYDFEFILIND
                     GSTDKTVETAEQNKESLNLTILSHGTNQGPGYAFGTGFEYLAGKLKNEDLVITMEGDN
                     TSRIETLNRMIERQIREKDDVVLASPYAYGGGMQNTSFYRMMLSHVANGLVKEFLGIH
                     GIHTMSSFFRVHTGDIILRLQQRFGSRIMERKGFESMIEMLKKIIILNASVSEVAMKL
                     DTSLRAGKSKMKVMKTIRGYLRLFTLAGKWK"
BASE COUNT          615 a          300 c          461 g          515 t
ORIGIN      
        1 atttttgaac cctcgcaaga gtgtttagca gtaatttctg cagtatctga aagggaagga
       61 aaaaagtgaa gaaaattctg attaccggtg tagccggttt tattgggtca aatgtgtgca
      121 ggcatctgct aggaaaagga tatcatgtta ttgggataga caatctttct caaagtgacg
      181 gaaagaatat gaatgagttt ctgaatcatt cagagtttga attttacaga gtggatatta
      241 tgaatgagtc ttctgttctt cgcgctgctc atggatgtag tgggattatt catatggctg
      301 catataaaat ccccaggtat tccgatgcat tagatacatt gaaaatcaac gggatcggat
      361 cagaaaattt agtaaaagct gccattgaaa ataatgccag acttgtagca gcttccactt
      421 cggatgtata tggaatgaat cctgaacttc catttaagga ggattcttcc aatttagtga
      481 tgggcggacc gaaagtaaga agatgggcct atgcaatatc aaaaatgttt gaagagcaga
      541 tgatctatgc ctatggtgaa agaatgaatt tggattttgc actaattcgg ttttttgggg
      601 gttacggacc ggggcagaac ctgacttggt ggggcggacc tcagtcagtt ttcataaaca
      661 aggcactcga tgatgaagag attgaagttc acggtgacgg tcagcagacc agaagtttta
      721 ctcatgtctc tgatcacgtg gacggagtga ccagatgtct ggaagtggaa aaagccaggg
      781 gagaagtatt taacatcgga aacgaccgag agatcactat tgaaaacctg gcaaagctga
      841 tctggaaaat gattcgtccg aatgatcctc ctaaaattaa aatgatccct tatgaaactt
      901 ttggaaagta tcaggatgtg atgagaagaa ttcctgatct gacaaagtcc aaaagcattc
      961 ttggatttga gccaaaagtg aaaatggaag atggattgaa gactgcaatt gaatggcaga
     1021 tcaagagaag aaaagagcta ggaatgactg accctaagtc tggatgcgtt tttaaagtat
     1081 gatgaaaaaa atatatatta ttactcctgt tttaaacgaa gagccgaaca tacttaggct
     1141 cctttccgga tggaaaaaag tcaagtccga tctgaaggaa tatgattttg agtttattct
     1201 gatcaatgac ggaagcacgg acaagacagt tgaaaccgca gagcagaata aagaatcatt
     1261 aaatcttact atactgagtc atgggaccaa tcaaggtcca ggttatgctt ttggcacagg
     1321 atttgaatac ttggctggaa aactgaagaa tgaggatttg gtgattacaa tggaaggaga
     1381 caatacttct agaatagaaa cacttaacag gatgattgaa agacagatcc gcgaaaaaga
     1441 tgatgttgtg ctcgcctcgc cgtatgctta cggcggcgga atgcagaaca ctagttttta
     1501 tagaatgatg ctcagtcatg tggctaacgg gcttgtcaag gaatttctcg gcattcatgg
     1561 gattcataca atgagttctt ttttccgggt tcatacaggt gatataattc tccgtcttca
     1621 gcagagattc ggcagcagaa ttatggaaag aaaaggcttt gaatccatga ttgaaatgct
     1681 aaaaaaaatt attatactca atgcttctgt ttcggaagta gcaatgaagc tggatacttc
     1741 tcttcgtgca gggaaaagca aaatgaaggt tatgaagaca atcagaggtt atctcaggct
     1801 gttcactctg gcaggaaagt ggaaataaga acaagtgatt gaatttctgg aagacatcca
     1861 tcggtacagg gaaatcctct gggatcttgt g
//
LOCUS       DAOQGJ010000787         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42764_length_1891_cov_3.050109, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000787 DAOQGJ010000000
VERSION     DAOQGJ010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42764_length_1891_cov_3.050109"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..>1891)
                     /locus_tag="PK683_12220"
     CDS             complement(89..>1891)
                     /locus_tag="PK683_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09258.1"
                     /translation="GFWLDSCDKFPQISSMYRFVPLSVQNEYRIIAAHSDLALTAAGK
                     GKNVIQKKVSDSPDQILILELAGSDRIRLRFKSSGLYLGIWGGEFRQGALAVEADPAS
                     NGSDIFVLSSELSSSEMDQIKKAKSLSSEAQKLIRAGDIDKGCGLMSEASKGTLNDGW
                     VLYESGYWLLHFCGKAETALTAAEKAVRFQNVRPAVLALLTNIYSSLQKWEAALKTAD
                     ESLNFWKDQTLKTGADSASIKNAQNELAGAYGRKANIYNQKAEWEKAVSTADEGFSLF
                     PDSSNPELIYAKCEPLHIEAGQKLSSDDYSSSMVLIQKCIDLYERNKSLSSLQNGRYK
                     DLKLALSLLAKRKKMGSVVPVTVQKTLGFIVSEIDIDQEIRGTRIRAKDRIRKDQVYR
                     LQAGQQWFRLYIEVMTEGKLSIQFKNTEAGKAVRLNVTVLKEPSYILEIDPDSISPSI
                     DSVLLEHQNWDNFIFYWNGQTSGINKGTGIGMYSWPFTMAGINLGQPRGWSVMGTNPE
                     MYGVFDWLLHHEYGHLLEAMSQISFVHCNDLTEKDKLLPVYSSWDKNCSTIYDWVLKD
                     GFRKMYGDLNWSRRYPANITQEDLQKMKKSVKSN"
BASE COUNT          488 a          457 c          367 g          579 t
ORIGIN      
        1 aatgctttct gaaaattatc gcatctagtc agcatattgt atttgcgtaa ggtgagttaa
       61 taagaatcag gcagaatagc agaatttctt aattactttt tactgatttt ttcattttct
      121 gcagatcttc ctgcgttata tttgcgggat atcttctgct ccagtttaag tctccgtaca
      181 ttttacggaa tccatcctta agaacccagt cgtaaattgt actgcagttt ttatcccagg
      241 acgaatagac aggaagaagt ttatcctttt cagtcagatc attgcagtga acaaaactga
      301 tctgagacat tgcttccaga agatgtccgt attcatggtg cagaagccag tcaaatacac
      361 cgtacatctc cgggttggtt cccatgacac tccatcccct gggctgtcct aaattgatac
      421 ctgccatagt aaacggccag gaatacattc ctatccctgt tcctttattt atcccgctgg
      481 tctgcccgtt ccagtaaaat atgaaattgt cccagttctg atgttcaagc agaacagaat
      541 caatgctggg cgaaatggag tctggatcaa tttcaagtat atatgaaggt tctttgagca
      601 ccgtcacatt gagcctgaca gctttccctg cctctgtatt tttgaactga atggaaagtt
      661 ttccctcagt catcacttca atataaagcc tgaaccactg ctgtcccgcc tgaagacgat
      721 aaacctgatc ctttctgatt ctgtcctttg ctctgatccg agtgcctctg atttcctgat
      781 caatatctat ttcggaaaca atgaatccca gtgttttctg cacagttaca ggaacgacag
      841 aacccatttt ctttcttttt gcgagcagac tcagtgcgag tttcagatcc ttgtatcttc
      901 cattctgcag actggagagc gacttgttcc tttcataaag atctatacat ttctgtataa
      961 gaaccatact cgaactgtaa tcgtctgagg aaagtttctg accggcctca atatggagag
     1021 gttcacattt tgcgtagatc agttcaggat tggaagaatc cgggaaaaga gaaaaacctt
     1081 catcagcagt cgacacagct ttctcccact ctgctttttg attataaata tttgcctttc
     1141 tgccgtaggc gccagccagt tcattttgag cattttttat tgaagcactg tctgcccctg
     1201 tttttaatgt ctgatccttc cagaagttca aagactcatc tgctgtcttc agtgcagcct
     1261 cccatttctg aagactggaa taaatatttg tcagcagggc cagcacagca ggtcgaacat
     1321 tctgaaagcg gactgccttt tctgcagcag tgagagcagt ctccgctttt ccgcagaaat
     1381 gtaaaagcca gtagccggac tcataaagaa cccatccgtc atttaaagtc cctttggatg
     1441 cttctgacat taatccgcat cctttgtcta tatctcctgc tctgatcagt ttctgagctt
     1501 cggaagacag cgacttcgct ttctttatct gatccatttc ggatgaactt agctctgaac
     1561 tcagaacaaa aatatcggaa ccgttggagg cagggtctgc ctcaaccgcc aatgctccct
     1621 gtctgaattc tccgccccat ataccgagat acagtccaga gcttttgaac cttaaccgta
     1681 ttctgtcaga tccggctaat tccagaataa ggatctgatc cggactgtcg gaaacctttt
     1741 tctgaattac attttttccc ttccctgcgg cagtcagcgc cagatcagaa tgtgcagcaa
     1801 tgattctgta ttcgttctgc actgaaagag gtacaaacct gtacatactg ctgatctgag
     1861 gaaacttgtc acagctgtcc agccagaacc c
//
LOCUS       DAOQGJ010000788         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42766_length_1891_cov_2.995643, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000788 DAOQGJ010000000
VERSION     DAOQGJ010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42766_length_1891_cov_2.995643"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK683_12225"
     CDS             <1..416
                     /locus_tag="PK683_12225"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH09259.1"
                     /translation="MTKSSGHIFFKHYLVHELQRILPGQFFIQTEDSIRLGESVLEPD
                     ISIIAGSFMDYRNSIPEAARLIAEISVSSLKYDREKARVYAKAKVPEYWIIDVENRKV
                     EVYSDPENEIYKSKIIYNFLDEIPVFERTVSLKNA"
     gene            complement(459..1157)
                     /locus_tag="PK683_12230"
     CDS             complement(459..1157)
                     /locus_tag="PK683_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09260.1"
                     /translation="MALNENDEPEYDIELSLDGFSGEESLQSILSWPEQLQKVRPIRV
                     LLFLGGMLDQQWSGQNISRMKIVNAIGDFFAEHREQMRIVWISLSETEIGDNACAELL
                     KRILEPGTPELLRLDFSCFGGITMAQHYKFGFGPQCRSALLNILRKKLCPNLEQLSLN
                     GCRLGRTYGQFEGKGFVPDYLPGESDFIPDFLTMLPISLPRLSYLNMEYCSLSEEEKT
                     LTASFRTEHLKILL"
     gene            1535..>1891
                     /locus_tag="PK683_12235"
     CDS             1535..>1891
                     /locus_tag="PK683_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09261.1"
                     /translation="MQDEKPKYEFLDRPSIPMDDRLKELGDALLDFIEESESLPEKEK
                     ETALYIGRMEKTGENLADAELIQFYWGYSSGEKNSVSLDSLRKMKFENSLDSLIDYPK
                     GFRAAIYFIFPLIECIR"
BASE COUNT          559 a          393 c          425 g          514 t
ORIGIN      
        1 aaatgactaa aagcagcgga catatttttt tcaagcatta cctggttcac gagcttcaga
       61 gaattctgcc cggccagttt tttattcaga ctgaagacag tatccgtcta ggagaatccg
      121 tgcttgaacc ggatatctca ataatcgccg gcagctttat ggattacagg aattccattc
      181 cagaagcagc aagactcatt gctgaaatat ctgtttcttc tcttaaatat gacagggaaa
      241 aagcaagggt atatgcaaaa gcaaaggttc cggaatactg gatcattgat gtggaaaaca
      301 gaaaggtaga ggtgtattca gatcctgaaa acgaaatata taaaagtaaa ataatttata
      361 attttttgga tgaaattccc gtttttgaaa ggaccgtttc tctgaagaat gcctgattcc
      421 ggcatctcaa gcagcgacag cgatgaccgt tctgctcctt acagaagtat cttcagatgt
      481 tctgtgcgga aggacgctgt aagagttttt tcttcctcac tgagactgca gtactccata
      541 ttcagatagg acagacgggg aagagaaatc ggcagcattg tgaggaaatc cgggataaaa
      601 tcagattcac ccggcaggta gtccggaaca aaacctttcc cttcaaactg tccatacgta
      661 cggccaagac ggcatccgtt cagactgagc tgttccagat taggacagag cttttttctc
      721 agtatattca aaagagcaga acggcactga ggcccaaacc caaacttgta atgctgtgcc
      781 atagtaattc cgccaaaaca gctgaagtcc agacggagaa gttccggtgt gccaggttcc
      841 agaatgcgct ttaaaagttc cgcacaggcg ttgtctccga tttcagtttc cgagagtgaa
      901 atccatacaa tcctcatctg ctccctgtgt tctgcaaaaa aatctccaat tgcatttaca
      961 atcttcatcc ggctgatatt ctgtccgctc cactgctgat caagcattcc tcctaaaaaa
     1021 agaaggacac gaatcggcct gaccttttga agctgttccg gccagcttag gatggactga
     1081 agactttctt ctcctgaaaa accgtcgagg gaaagttcta tatcatattc cggttcatca
     1141 ttttcattga gtgccatacc atcactcttc tgcaggcttt tcctgatgca aggtgaattt
     1201 atcagtcaag gatggatcct ttttacaaaa tgcaattgac acagtctgcc ttccgaattt
     1261 cctcttagag agacaggagt ctccatacgg caggcttaca gcagtgcgat gtcattaaga
     1321 taagagcttt taaccgcgaa gaacgtaaag attacgaagc aagcgaacca aattccttgc
     1381 ttcgtgttct ctttgcaccg gagtttatgc tgagcagttc agtgtgtcga agtatggtta
     1441 atgcatcggg tttggaatct gtgaaattta tactgttttt tataaaaaag tatatcatta
     1501 agcggaagaa tttatttaaa ctgaggagag gattatgcag gatgaaaagc cgaagtacga
     1561 gtttctggac agaccttcca ttcctatgga tgaccggctg aaagagctgg gagatgcatt
     1621 gctggatttt atagaggaat ctgaaagtct tcctgagaaa gaaaaggaaa cggcacttta
     1681 tataggccgg atggaaaaaa caggagagaa ccttgcagat gcagaactga tccagtttta
     1741 ctgggggtat tcttccggag agaagaactc tgtcagcctg gattcactca ggaaaatgaa
     1801 gttcgaaaac agtctggaca gtctcatcga ctatccgaaa gggttcaggg ccgccatcta
     1861 ttttatattt ccgctgattg aatgtattcg g
//
LOCUS       DAOQGJ010000789         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42799_length_1890_cov_2.826158, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000789 DAOQGJ010000000
VERSION     DAOQGJ010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42799_length_1890_cov_2.826158"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PK683_12240"
     CDS             complement(<1..633)
                     /locus_tag="PK683_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09262.1"
                     /translation="MMADLKNAENEELEQDQLEEDGELLPDEEQENKSLSIKEKLILG
                     LLGLSAFLLSFLMLFPIEEIVRFMIGRVSHEAGFVLDFKKLDFPLFGKKSFDAVYFLN
                     KDGIEFKSEELLADLSLKQLYSARLDGEVQATPFEIDTHDLTVTGSSLKSKVNLSNID
                     EGFSAAAGSVEILMNASKITRFPVIPFVGDLSGTQIKTLNSFQIFFTLLVR"
     gene            complement(630..1148)
                     /locus_tag="PK683_12245"
     CDS             complement(630..1148)
                     /locus_tag="PK683_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09263.1"
                     /translation="MLSKLDEREKLAVISFIAVLILLTVSVIFKYLYEMRSELSNRVI
                     ESRASFPVLERAIKDYNYFRSLKSGDDESSAVILQKIDQLIVKYAMKDQLQKISEGNV
                     PILKQYNKYTIELTFRSVVLQDMMKMIYDVEMNKQVNCKVDYFSFRKSIQGKELYDVN
                     IKFAYYSRIGKK"
     gene            complement(1138..>1890)
                     /locus_tag="PK683_12250"
     CDS             complement(1138..>1890)
                     /locus_tag="PK683_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09264.1"
                     /translation="TEKYLSDLTGIQFKRYDFLENNDEVFINCLSMGYHYRLPAKDQI
                     NFLTPEFSRKLNKNAFRLSNFKVHISLSAAAFFILSTVFITGIFIDRKKIDANHSVLS
                     QRYKEGFGKDLGEDDDPIEKALSEVKTEQKKSEIVRLFLSKDSILDLIHEMTMLFPAK
                     EEFNFVMDNFIYDGTEIQIQGKVDSYIDAENLKEALAKSKKFKNFKLDKNLIPNVPKL
                     GVNIRLKMELVSGSQEKPNEKNKKGDGGGSAE"
BASE COUNT          520 a          422 c          348 g          600 t
ORIGIN      
        1 ccttactaaa agagtgaaga agatttgaaa agaattcaaa gtcttgatct gagtgccgga
       61 aagatctccc acaaacggaa tgacaggaaa cctggtgatc tttgaggcat tcatcagtat
      121 ttccacagaa ccggctgcag ccgaaaatcc ctcatcaata ttgcttagat tcactttgga
      181 cttgagggaa cttcctgtta cagtcagatc atgggtatct atttcaaaag gagtggcctg
      241 cacttccccg tccagtctgg cagaataaag ctgcttcagg ctcagatcag ccagaagttc
      301 ttccgacttg aattcaatcc cgtccttgtt caggaaatag actgcatcaa agcttttctt
      361 tccaaaaaga ggaaaatcca gttttttgaa atccagaaca aaaccagcct catgtgaaac
      421 ccggccgatc ataaaacgca caatttcttc tatgggaaaa agcatcagaa atgaaagaag
      481 gaatgcactt aatccaagca ggccgagaat cagcttttcc tttatgctta atgatttgtt
      541 ctcctgctcc tcgtccggaa gaagctcgcc gtcttcttcg agctgatcct gttccagttc
      601 ttcattttct gcattcttca gatcagccat catttcttac ctattcttga atagtatgcg
      661 aacttaatat tgacgtcata gagttctttt ccctgaatgg acttcctgaa actgaagtaa
      721 tccactttgc agttcacctg cttgttcatt tccacatcat atatcatttt catcatatcc
      781 tgaagaacca cagatctgaa tgtaagctct attgtatatt tattgtactg tttcaggatg
      841 ggaacatttc cttcgctgat tttctgaagc tgatccttca tcgcatactt tacaatcagc
      901 tggtcgattt tctgaaggat gactgcagag gattcgtcat ctccagactt gagtgaacgg
      961 aaataattgt aatccttgat tgctctttcc agaacaggaa aactggctct tgattcaatt
     1021 accctgtttg agagttctga acgcatttca taaagatatt tgaatatcac tgatactgtc
     1081 agcagtatga gcactgcaat gaaactgatg actgccagtt tttccctttc gtcaagctta
     1141 ctcagcactt ccgccaccgt cccctttttt gtttttctcg ttcggctttt cctgacttcc
     1201 ggacacaagt tccattttca gacgtatgtt cactcccagt ttgggaacat tgggaatcag
     1261 gttcttgtcc agcttgaaat tcttgaattt ctttgacttg gcaagagcct ctttcagatt
     1321 ttctgcatcg atatacgaat ccactttccc ctggatctgt atttccgttc cgtcataaat
     1381 aaaattgtcc attacaaaat tgaattcttc ctttgcagga aaaagcattg tcatttcgtg
     1441 aatcagatca agtatgctgt ccttgctcag gaaaagtctg acaatctccg atttcttctg
     1501 ttctgtcttt acctcagaaa gagccttttc gataggatca tcatcttctc cgagatcctt
     1561 gccgaaacct tccttgtatc tttgagacag gacagaatga tttgcatcta tctttttcct
     1621 gtcaatgaag atccctgtga tgaagacggt ggacagaatg aaaaatgcag ccgctgacag
     1681 agaaatatgc accttgaaat tggaaagtct gaatgcgttt ttattgagct ttctggaaaa
     1741 ctcaggtgtc agaaaattaa tctgatcctt ggccggaagc ctgtaatgat accccattga
     1801 aagacagttg atgaatactt catcattatt ttccagaaaa tcatatctct taaactgaat
     1861 tcctgtgaga tcggaaagat atttctcagt
//
LOCUS       DAOQGJ010000790         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42834_length_1889_cov_3.792257, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000790 DAOQGJ010000000
VERSION     DAOQGJ010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42834_length_1889_cov_3.792257"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PK683_12255"
     CDS             <1..500
                     /locus_tag="PK683_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09265.1"
                     /translation="TNAGKTTFSKKFEELGYVCFHYDCRYRDSLHLSDQYGNILNYIF
                     SVHSDILGEFKKYKNRSIVYEGVHFLFKNILCSVLNSVREREISFIVFDFPFQELWKR
                     LRARVRDQNRRKYRLQMYCLLAATWLLFKISKSAYCRLIPQNSLYFIRSQKDLDEFYI
                     RCRNR"
     gene            complement(525..1352)
                     /locus_tag="PK683_12260"
     CDS             complement(525..1352)
                     /locus_tag="PK683_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09266.1"
                     /translation="MSNKKKKDHNKSEAQECSHCGRPDTASYSLWDSSDPFIKAFQIE
                     KKLKFGDLVSCPECKSRWLKLPNPRNKESNYVSLDFIPDNSVPKLTEWDAMDLKVTAA
                     QKKVLKEIGATPPDAYTNGSEYIRFPCRCILKDGRKLDMCILEFRRVLPETDGDRELI
                     FLNDVKEILPSEHTLSKKVRYATTRAEEIRNSYAPTVVKLPTGGKFIFNWTTDFFASK
                     KIKGSEITEVLMEDPFEIKTADADEIGQLIPYNQTVIYAHWDDELLEFELNENNLKE"
     gene            1548..1799
                     /locus_tag="PK683_12265"
     CDS             1548..1799
                     /locus_tag="PK683_12265"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNH09267.1"
                     /translation="MKPQNPPPMSIWNLSSLGTEFGIIIVGFILAGSWLEERFSFSPW
                     GVMVGALLGFGIGIFHLVKRTDEFNRSNEKKKDPSDSAP"
BASE COUNT          504 a          377 c          398 g          610 t
ORIGIN      
        1 cgacgaatgc aggcaagaca acattttcaa agaaatttga agaacttggt tatgtatgtt
       61 ttcattatga ctgcaggtac agagacagtc tgcacctttc agatcaatat ggaaatattc
      121 tgaactatat tttttcagtt cacagcgata ttctcggaga atttaaaaaa tataagaaca
      181 gaagtattgt ttatgaagga gttcattttc tgttcaagaa tattctgtgc agtgtcctga
      241 actctgtcag agagagagaa atcagcttta ttgtatttga ttttccattt caggaacttt
      301 ggaaacgtct gcgggcaaga gtaagggatc agaaccgcag aaaatacaga ctgcagatgt
      361 actgtctgct tgcagcaaca tggctgctgt ttaaaatcag taaatctgcc tactgcaggc
      421 tgatccctca aaacagtctg tattttatca gatctcagaa agacttggat gagttctaca
      481 ttcgatgcag gaaccgctga tctgacgagg acctaattaa tgaatcattc cttcagattg
      541 ttctcattca gttcaaactc cagaagttca tcgtcccagt gggcataaat tacagtctga
      601 ttgtagggaa tcaattgtcc tatctcatct gcatctgctg ttttgatctc aaaaggatct
      661 tccataagaa cctctgtaat ttcagaacct ttgatttttt ttgaagcaaa gaaatctgtt
      721 gtccagttga aaatgaattt tcctcctgta ggaagtttta caactgtggg ggcataactg
      781 tttctgatct cctcagcacg agttgttgca taacgcactt ttttggaaag tgtatgttcg
      841 gacggaagga tttcttttac atcgttcagg aaaataagct cccggtctcc gtcggtttca
      901 ggcagtaccc ttctgaattc cagaatgcac atatcgagtt ttcttccgtc tttcagaatg
      961 catctgcagg gaaagcggat atattccgat ccgtttgtat aggcatccgg aggagtcgca
     1021 ccgatttctt tcaaaacttt tttctgtgct gcagtgactt tcaaatccat cgcatcccat
     1081 tctgtaagct tcggtactga gttgtccgga ataaaatcca ggctgacata attggattcc
     1141 ttgtttctcg gatttggcag tttgagccag cggcttttgc actctggaca ggaaaccaga
     1201 tcaccgaatt tcaatttctt ttcaatctga aaagccttaa taaaagggtc actgctgtcc
     1261 caaaggctgt aactcgcagt atccggcctt ccgcaatgac tgcattcctg tgcctcactc
     1321 ttgttgtggt ctttcttctt tttgttggac atgcgggata ttttatgcag tgtctgtttt
     1381 tgtaaagaaa attaacgtaa attcagtact gagtgcaatg aatttctcgg cctgtgcgca
     1441 ttccatttgt ctttgcttaa gtgaagggtt ttttagctta ggataaagaa ttcattcccc
     1501 ggattctttc ttgcagaaat gaggaatgat tattttctgt acttgtaatg aaaccgcaga
     1561 atcctcctcc tatgagcatc tggaatctct caagcctggg aacagagttt ggaattatta
     1621 tagtagggtt catccttgcc ggaagctggc tggaagaaag attttccttt tctccctggg
     1681 gagtaatggt cggcgctctg ctcggttttg gaataggaat atttcattta gtaaaaagga
     1741 ctgatgaatt caacagatca aatgaaaaga aaaaagatcc ttccgattct gctccttagc
     1801 attgtcatca tcactcagtt ctgtcggaga acggatgcag tttctgagcg tgtatgggta
     1861 attggaatcc cctccgatcc ttcttctgc
//
LOCUS       DAOQGJ010000791         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42835_length_1889_cov_3.785714, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000791 DAOQGJ010000000
VERSION     DAOQGJ010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42835_length_1889_cov_3.785714"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>1889
                     /locus_tag="PK683_12270"
     CDS             61..>1889
                     /locus_tag="PK683_12270"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNH09268.1"
                     /translation="MRVWSAVFLFLFLSYPLLSSENAVKGFIDLSSFNFREKNQSVSL
                     DGEWEFYWQKLVPPADFSGVNPPKPDLYSPFPLTWEKIPDQKFPAKGYGTYRLRIRLN
                     SLDTFVFYVYGFGTNYRMYVNGEKLTESGIPNEKEETSVPRMIPSVFAYTPHSADLDI
                     TVHVSNFHYRSGGAWYPIRIGSQTVIRNEIYYSLFLDILIFGSVFIMGIYHIGIYINR
                     RKSLEALYFGIFCLLISFRTLLTGEKILPLLNPNLSWEFLMKTEYLTFYLGTPLFINY
                     LYALFKDLVHYRMVLICNAIGVIFTLAVLFSNSYIYTHTVQAFQLFTLVYMVYGIIMV
                     SIGIRRKIPGTAIFLISLLLFSGTVINDILYNNQVVNTTNLAPLGFISFIFLQSFLLS
                     KNFSQAFIMSEQLGLELQSKNESLTKLDKLKDDFLANTSHELKTPLNGIIGLSESLID
                     GAAGPLPERAAYNLSMIITSGKRLSNLVNDILDFSKMKGNELKIDRKPVDIYSLVNLV
                     LSLSHILLGKKKIDLRNSIPIDFPSASGDENRIQQILYNLIGNAIKFTESGTVEISGK
                     INDSIMEISVTDTGIGIPEDKYDTIFKSFEQIDSASDRKYGGTG"
BASE COUNT          524 a          366 c          389 g          610 t
ORIGIN      
        1 ttgatggact tgctgaccat actgattgat tagtttacag gttcatttcg gggagaaaaa
       61 atgagagtct ggtccgcagt ttttctgttt ttatttctgt cgtatccttt attaagttca
      121 gagaatgcag tcaaggggtt tattgatttg agttctttca atttcaggga gaaaaatcag
      181 tccgtttctc tggacgggga atgggagttt tactggcaga agttagttcc gcctgccgat
      241 ttttccggtg tgaatccgcc taaacctgat ttatattctc catttcctct cacatgggaa
      301 aaaattccgg accaaaaatt tcctgcgaaa ggatacggga cttacagact cagaattagg
      361 ctgaattcac tggatacatt tgtgttttat gtgtacggtt tcgggacaaa ttacagaatg
      421 tatgtgaacg gagaaaaact gacagaatcc ggaattccaa atgaaaaaga agaaacttct
      481 gtccctcgaa tgattccttc tgtttttgct tatactcctc attcagctga tctggacatt
      541 acagtccatg tttccaattt ccattacaga tcgggcggag cctggtaccc gattcgaatc
      601 ggaagtcaga ctgtaatcag aaatgaaatc tattattccc tgtttctcga tatacttata
      661 ttcggaagcg tatttattat gggaatttat catataggta tttatatcaa cagaagaaaa
      721 tctctggaag ccctgtattt cggaatattc tgccttctga tcagtttcag aacccttttg
      781 actggagaaa agattcttcc tctcctgaat ccgaatctgt cttgggaatt cctgatgaaa
      841 accgagtatc tgacctttta tctgggaaca ccgctgttta tcaattatct ctacgctctt
      901 ttcaaggatc ttgttcatta tcgaatggtt ctgatctgca atgctattgg ggtgattttt
      961 actctggccg ttcttttttc caattcttat atatatacac atactgttca ggcatttcag
     1021 ctctttactc ttgtatatat ggtttatgga attataatgg tgagcattgg aatcagacgg
     1081 aagataccgg gaactgctat tttcctcatc agtctgctcc tgttttccgg cacagtcatt
     1141 aatgatattc tgtacaataa tcaggttgtc aatacaacaa acctggcacc gctgggtttc
     1201 atctccttca tttttctgca gagttttctt ctttccaaga atttttctca ggcattcatc
     1261 atgtctgagc agcttggact tgagcttcag agcaagaatg aatcactgac aaaactggat
     1321 aaactcaaag atgattttct tgcaaacact tcccatgaac tgaagactcc gctgaacggg
     1381 atcattgggc tgagcgagtc tctgattgac ggggcagcag ggccgctccc tgagagggct
     1441 gcctataatc tctcaatgat tattacgagc ggaaagaggc tctcaaatct tgtaaatgat
     1501 attctggatt tttccaagat gaagggaaat gaactgaaaa ttgacagaaa gcctgtggat
     1561 atctattctc ttgtaaatct tgttctgtct ctttctcata tcctgctcgg taagaaaaaa
     1621 attgatctgc gcaattccat tcctatagat tttccttctg catcgggaga tgaaaacagg
     1681 attcagcaga tattgtacaa cctgataggg aatgcaatta aattcaccga aagtggcact
     1741 gtggagattt caggaaaaat taatgattct ataatggaga tttcagtcac tgatacagga
     1801 ataggaattc ctgaggacaa gtatgatact atattcaagt ccttcgaaca gattgattct
     1861 gcaagtgacc gaaaatacgg cgggacggg
//
LOCUS       DAOQGJ010000792         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42837_length_1889_cov_3.731189, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000792 DAOQGJ010000000
VERSION     DAOQGJ010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42837_length_1889_cov_3.731189"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1662)
                     /locus_tag="PK683_12275"
     CDS             complement(37..1662)
                     /locus_tag="PK683_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNH09269.1"
                     /translation="MDFSFTFQKSDVGGTKPSARQDHSAVRHDGFMYIFGGRNSKGEF
                     LNDTFCLDLAEKNWTKISTKGTKPSPRYGHSATVYNGQMFICGGVTGDKKNDTEIYVL
                     DLTAFQWSKFAVEGEMQMPDAVFGAVLSAPLWLLFGDSYACAVNLENRKKISLDIRKK
                     KPEPVPGGGLVLYNKKIYHFSGESKQTGILDLGSVKAQSEFVDGESYNEPKPDMFSLN
                     WVSAQTEGRKYSDRTVHGFGRLGGTVAVYGGRDSEDSLLNSVMILSLPEGKSREVTVQ
                     NLEIQDADSLAGHSTVQYGNSLFLFGGESTSGLSNDTYELLAVPAKPRKTKSKRVVPF
                     WDKERPALPEDLPVATVLLVNSDKWEVPSSYFRATAGQSNYLNWLELDYPDIRAYLKR
                     GDIVENVRWSGDRSYGIQFYDGHTLVPVFKEYDDYGSLSAEFKMFREFHPRYWNELNV
                     DDRFAPGKKSQFYWHSNGVCQIMDKKAVQTAAKAGVFESEYGTYIIVNYQGLKYAVLQ
                     NCPKLKAGQLGFLYDDPKLAKSLNADFVVGPER"
BASE COUNT          524 a          459 c          373 g          533 t
ORIGIN      
        1 atgctgagtc cggaaagata tgatgtccga attgcttcac ctctctggac cgaccacaaa
       61 atcagcgtta agtgatttgg ccaatttcgg atcatcataa agaaatccga gctgacccgc
      121 cttcagttta ggacagttct gcaatacagc atatttcaaa ccctgataat ttacgatgat
      181 ataagtcccg tattcagatt cgaatacacc tgctttggct gcggtctgta ctgctttttt
      241 atccatgatc tgacagaccc cgttcgagtg ccagtagaac tggctcttct ttcccggagc
      301 aaaccggtca tccacattca gttcattcca gtaacgggga tgaaactctc tgaacatttt
      361 gaattctgca ctcagacttc cgtaatcatc atattccttg aaaactggaa caagtgtatg
      421 accgtcataa aactgaatgc cgtaagaacg gtcgcctgac catcggacat tttccacaat
      481 gtcaccgcgc ttcagataag cccggatatc cgggtagtcc agttcaagcc agttgagata
      541 attggactga cctgccgtcg ctctgaaata ggaagaagga acttcccatt tgtccgaatt
      601 gaccagaagc actgtggcaa caggaagatc ttccggaagt gctggtcttt ccttatccca
      661 aaaaggaaca actctctttg acttggtctt tctgggtttt gctggaacag cgagcagttc
      721 gtaagtgtca ttagaaagtc cggaagtgct ctctcccccg aaaaggaaaa gactgtttcc
      781 atactgcaca gtggaatgcc ctgccagtga gtctgcatcc tgaatttcca agttttgaac
      841 agtgacttcc ctgctttttc cttcaggtaa tgacagtatc attacggagt tcagcagact
      901 gtcttctgaa tctcttccgc cgtaaaccgc aactgtaccg ccaagccgtc caaatccgtg
      961 aacagtcctg tctgaatatt ttctgccttc tgtctgagca gaaacccagt taagactgaa
     1021 catatccggt ttaggttcat tgtaactttc accgtcaaca aattctgatt gtgctttcac
     1081 gctgccaaga tccagaattc cagtctgctt tgattctccg gaaaaatgat atattttttt
     1141 attatataaa acaagtcctc cgccgggcac aggttcaggc ttcttctttc tgatatcaag
     1201 agagatcttt ttgcgattct ccagattgac tgcacaggca taagagtctc cgaaaagaag
     1261 ccagagagga gcagaaagaa ccgccccaaa gacagcatca ggcatttgca tttctccttc
     1321 cacggcaaac ttcgaccatt gaaatgcagt cagatccaga acataaattt ctgtatcatt
     1381 ttttttatct ccagtcactc ctccgcagat gaacatctgt ccgttataaa cagtcgcaga
     1441 gtgtccgtac cttggagaag gtttcgttcc ctttgtactg atcttagtcc agtttttttc
     1501 agcaaggtca agacagaaag tgtcgttcaa gaactctccc ttgctgttcc tccctccaaa
     1561 aatatacata aatccgtcat ggcggacagc actgtgatcc tgtcttgcgg aaggtttagt
     1621 tcccccgaca tccgatttct gaaaggtaaa tgaaaaatcc ataacctaga tacttttatt
     1681 gagctcggat tttattcaat ttttttatag tttaaaacag gatgaatgtc tgatcctgcc
     1741 ggccgaacta tcaataaata ctctgcaaaa atggatgttg agacagaatt accattctgc
     1801 tttacagaaa aagcagccgg cagaaaacag aacaatgaaa tccagacgct cattcaatac
     1861 ttcgggtccg aacattccgg aaatgcact
//
LOCUS       DAOQGJ010000793         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42841_length_1889_cov_3.328790, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000793 DAOQGJ010000000
VERSION     DAOQGJ010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42841_length_1889_cov_3.328790"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK683_12280"
     CDS             complement(<1..353)
                     /locus_tag="PK683_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09270.1"
                     /translation="MNLFRRIFFLLIFLAASSVFSEKGIKDKPYTVEYYYKAKWGYAD
                     EFIELYKKNHYPVMKKEMEIGRILKLSFTKPRIHFTEDSRWDFKVTIVWKNIQTTDDG
                     FDEKALMLQLFPDQQT"
     gene            complement(355..894)
                     /locus_tag="PK683_12285"
     CDS             complement(355..894)
                     /locus_tag="PK683_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HNH09271.1"
                     /translation="MESTLTIMPETDPSKKEIFKGYDVLREKMASLGIGYERWEAEKP
                     LTDTASPEEVAAAYKTSIDRILAKGYKTFDVVSMHPESPNAKEARAKFISEHTHSDDE
                     VRFFVDGSGMFYIHKAGLVCMMLCTKGDFISLPAGTTHWFDMGPKPLFKAIRVFNSPD
                     GWVGNFTGDSIPDRFPKYE"
     gene            1387..>1889
                     /locus_tag="PK683_12290"
     CDS             1387..>1889
                     /locus_tag="PK683_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09272.1"
                     /translation="MSETEYNEEESETGDEAPDLNEWLEEKPSIAGAIFWENKKGAVS
                     YSKWDDDKKESLQEIFASVWNEETVFEEDPVPNKAKLKKGDEVVQTMDEDLAWNIFAS
                     YLSQSLAMEIKEKLEWSLEDMSAQGLAELLDGRKMFVYDSEKNEFSIDYRFGYVLYCD
                     PVTALQF"
BASE COUNT          532 a          392 c          396 g          569 t
ORIGIN      
        1 gtctgctgat ccggaaaaag ctgaagcata agagcctttt catcaaaccc gtcatccgtt
       61 gtctgaatat tcttccatac aattgtgacc ttgaaatccc atctgctgtc ttccgtaaaa
      121 tgaatccggg gctttgtaaa agagagcttg agaattcttc ctatctccat ttcctttttc
      181 attacgggat aatggttttt cttgtacaat tcgatgaatt catcagcata accccacttg
      241 gctttgtagt agtactccac tgtgtagggt ttatccttga ttcctttctc cgaaaaaacg
      301 gaagaagctg ccaggaaaat aagcaggaaa aatatacgcc gaaacagatt catattattc
      361 gtattttgga aatctgtcgg gaatactgtc ccctgtgaag tttcctaccc atccgtcagg
      421 gctgttgaat acacggattg ccttgaataa aggttttggt cccatatcaa accagtgtgt
      481 tgtgcctgca ggaagactga tgaaatcacc cttggtgcag agcatcatac agaccagtcc
      541 tgctttgtgg atataaaaca ttccggaacc gtctacaaaa aaacgaacct catcatcact
      601 gtgagtatgt tcacttatga attttgctct tgcttctttt gcatttggtg attccggatg
      661 cattgaaacc acatcaaacg ttttgtatcc ttttgcaaga atcctgtcaa tggacgtttt
      721 gtatgcagct gctacttctt ccggggatgc tgtatcagtc agaggttttt ccgcttccca
      781 gcgttcataa cctatgccaa gacttgccat tttctcacga agtacatcat atcctttaaa
      841 tatctctttt ttgctcggat ctgtttccgg cataattgtc aatgtgcttt ccattaactg
      901 ctcctctaaa ttctatccgc tgattttgcc tctgcacagc cagggagcgt tctgctccgt
      961 acttagggca gggatcaaaa acctgcagtg aatgaatttc tgactttcat tcagttcaat
     1021 ccggtcagga caccttgcga ttcacagact cattccggaa tcgaactgcc tcggcctcgt
     1081 atgtaaagcc tcgcggagag tcgactggaa gtaaagagaa aaaatgactg ccgctgctga
     1141 gttaatccta ccggaggtga ggttgtgtaa aagcatacct cgatacaatt ttcaccgccg
     1201 gttacctgct tttacacagt cccaatccct atttagcttc tgattgaaga taatttcttg
     1261 attttttcaa caactcaaaa taagtttgta tctttttaag atgcttagta tctgttcatt
     1321 tacaataaaa ttcgggatat tctgccgact aaaaatcagc tgatgcctgc cggatgaagg
     1381 gaaaatatgt ctgagactga atataacgaa gaagaaagcg agactggaga cgaagctcct
     1441 gatttgaatg aatggcttga ggaaaaaccg tccatagcag gggcaatttt ctgggagaac
     1501 aaaaagggag cagtttccta ttccaaatgg gatgatgata aaaaagaaag tctgcaggaa
     1561 atattcgcgt ctgtctggaa cgaagaaact gtttttgaag aagatcctgt cccaaataag
     1621 gcaaaactga aaaaagggga cgaggtagtg cagactatgg atgaagacct tgcctggaac
     1681 atttttgcgt cctatctttc tcaaagtctt gcgatggaaa tcaaggaaaa actggaatgg
     1741 tctctggagg atatgtctgc acaaggactt gctgaacttc tggatggcag aaaaatgttt
     1801 gtatatgatt ctgaaaagaa cgagttcagc atagattata gatttggata tgttctctat
     1861 tgtgatcctg taacagctct tcagttttt
//
LOCUS       DAOQGJ010000794         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42849_length_1889_cov_2.578517, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000794 DAOQGJ010000000
VERSION     DAOQGJ010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42849_length_1889_cov_2.578517"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1567)
                     /locus_tag="PK683_12295"
     CDS             complement(8..1567)
                     /locus_tag="PK683_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09273.1"
                     /translation="MNKNTEKSESGINREHEFKYILPKDIFDLLESSNLEKISIGPDF
                     TVYFKEPRIKNRPYIYIDSDSRMLLNSGAQLSIRKRERDYFLTFKTGNDSLLGDFLTE
                     REENNLQIPESKGNLFWENRDCRVIQEFEPVQAAYDIMKSAGNEDLKELCSFMVHTKR
                     MTAALKGSDQELFEIAFDRIETSDSHFLYEMELEFKYSKNKISLPAAEQLEDIVLRML
                     SRTVEEKTGKKFDFNEFRTVLPKFRRVLEQTKRKERISMEVDEMHDWKDGSNNLLGFI
                     NGHLDMLKKSLFAVPVMLFVKPHEYKADELYKENKEELTKLRRLDIKKYNQELNAMYL
                     RSRKEIETFAEKYLTIALDSISSSQGRFSVHKFALPFSEYLLLFIFPSNAFSQTEGLS
                     VYQKVEALICETVLRKVIEISGDRDIYSKMQINFGLGNKDAVSSQQDGSDTRVLQIEF
                     FEFIPNQPELNSINTERLMNRCRLNLDFLNKYRNELIRFEGKGPLLAMKGRKNHSTAS
                     QTVILKLKGYL"
BASE COUNT          517 a          387 c          340 g          645 t
ORIGIN      
        1 gtcgttttca taaataccct ttcagtttga gaatgactgt ctggcttgca gtactgtgat
       61 tttttcgtcc tttcattgcc aaaagaggcc ccttgccttc aaagcggatc agttcatttc
      121 tgtacttgtt caaaaagtcc agattaagcc tgcacctgtt catcagtctt tccgtattga
      181 tcgaattgag ttcaggctga ttcggaatga attcaaagaa ttcgatctga agaacacgtg
      241 tatccgatcc gtcctgctga ctgctgactg catccttgtt tcccaacccg aaattgatct
      301 gcattttgct gtaaatgtcc ctgtctcccg atatctcaat cacttttcga aggacggtct
      361 cgcatatcag agcctctact ttctgataaa cgctcagtcc ttcagtctgt gaaaaagcat
      421 ttgaaggaaa aatgaaaaga agaagatatt ctgaaaaagg aagtgcaaat ttatgtacgg
      481 aaaatcttcc ctgagaactg ctgatgctgt cgagtgcaat cgtaagatat ttttcagcaa
      541 atgtctcaat ctcctttctg cttctcaaat acattgcatt gagttcctgg ttgtattttt
      601 tgatatccag tcttctgagt tttgtcagtt cttccttgtt ttccttgtac agttcatctg
      661 ccttgtattc atgaggcttg acaaagagca tcacaggaac tgcaaataat gattttttca
      721 gcatatccag atgtccgttg ataaatccga gaaggttgtt cgatccgtcc ttccagtcat
      781 gcatctcatc tacttccatc gatattcttt cttttctttt tgtctgttcc agaactcttc
      841 tgaatttagg aagaacagtt ctgaattcat tgaagtcaaa ctttttccct gtcttttctt
      901 ccactgttct ggacagcatt ctcagtacaa tgtcttccag ctgctccgct gcaggcaggc
      961 tgattttatt ttttgaatac ttgaactcaa gttccatttc atacagaaaa tgggaatcag
     1021 aggtttctat tctatcgaat gcaatttcaa acagctcctg atcgcttcct ttcagtgctg
     1081 cagtcatcct tttggtatga accataaaag agcatagttc tttcagatct tcatttccgg
     1141 cagacttcat tatatcgtat gcagcctgta ccggttcaaa ctcctgtatg accctgcagt
     1201 ctctgttttc ccagaacagg tttcccttag actcaggaat ctgaagattg ttttcctctc
     1261 tttctgtgag aaaatctcca agaagagaat catttcctgt cttgaaggtg agaaaatagt
     1321 ctctttccct cttccgaatg gaaagctgcg ccccactgtt taaaagcatt ctggaatcag
     1381 aatctatata aatgtaagga cggtttttta ttcttggttc cttaaaatag actgtaaaat
     1441 caggtccgat ggaaattttc tccaaattgg atgattccag cagatcaaat atatcctttg
     1501 gaagaatgta tttgaattca tgttctctgt ttatgccgct ttctgatttc tctgtgtttt
     1561 tattcactgt caatatttcc tctgactgga ttctgcaaag catttgctta aagcattctg
     1621 aacaatacat aaaaaaccgt atgttttaca aaatgtctat tcgaatgtaa taaaaaattc
     1681 tacagtcaat tgtattgaca atttacatat aaaattagaa ttgtaaagaa aatttttaga
     1741 agagtccggt atgagtgaag aaaaggcaga gcaggaatac tcagtttatt tcagaaattt
     1801 tttaagcaga gatgatgctt cagccggata tatagatcct gctgagagaa aaaacctaag
     1861 actgaagaga atgcgtcttg gcatttccg
//
LOCUS       DAOQGJ010000795         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_42958_length_1886_cov_2.726925, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000795 DAOQGJ010000000
VERSION     DAOQGJ010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42958_length_1886_cov_2.726925"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PK683_12300"
     CDS             <1..204
                     /locus_tag="PK683_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004764442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09274.1"
                     /translation="DRITARTISIEVGMQNSGLGAILAKNSFPSPLTAVPSAISSLFH
                     SLIASCLAWYWNYTDSKRKLETE"
     gene            complement(178..1488)
                     /locus_tag="PK683_12305"
     CDS             complement(178..1488)
                     /locus_tag="PK683_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09275.1"
                     /translation="MASPFNIRRSIVLTIAFGTAFTIVLALTPMKKKRGKTAPVSERA
                     VKSASSVPVYRLQPFFPASPDSQRAEREYFRIYPVLYRKESELRIIFQGAYTPDSERN
                     FEIRNLFWRNDSLLNSSSNPYTYYKDNDITFSAVTDSESRFLHKEIAGQKQYGMYHPV
                     PRLEADLKLLELSYRLSANVTANVRSGANDNYGDDRFQILPTTMAGLSFGNNKIVSAG
                     FMTGESRTANYYTNNVNMASPAQYLAREDQMLTKDTDRRNMFELHTNIAPTKNIQFQT
                     GVYNSNRAILNEGASEGARVAMQVGFRYLIFNLKYNFSSDNMMKSGFRADPSLNAKDY
                     GGVGLTFFLDTSRRYSLYLGNNFYNLMVPVRYSDGTGREAQPTSFAASFRGKSPLSNG
                     TFFLNFRNQYNKDQLYSNFGLFRVPIYSQINLDYATSLGLELSF"
     gene            complement(1488..>1886)
                     /locus_tag="PK683_12310"
     CDS             complement(1488..>1886)
                     /locus_tag="PK683_12310"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HNH09276.1"
                     /translation="IAVISALSGVEIPANIALTGSISQYGDILAVGAVALKLEGWFDI
                     SRILGSSKDSYKVFIPETNSKDLVLKPEIRDAVKKGKFNIFTFSHVSDIIPEVFGMEL
                     GRKEKDSKYTKGSLLRLIEDKLEKKTKEGE"
BASE COUNT          513 a          415 c          379 g          579 t
ORIGIN      
        1 gacagaatca ctgctcggac aatctccatc gaagtcggaa tgcagaattc aggccttggt
       61 gcaatccttg caaagaacag ttttcccagt cctctgactg cagttccttc agcaatttcc
      121 agtctgtttc attcactgat agcgagctgt ctggcctggt actggaatta cactgattca
      181 aaaagaaagc tcgagaccga gtgaggtagc atagtccagg ttgatctgag aatatatggg
      241 aactctgaaa agtccaaaat tggaataaag ctgatcctta ttgtactgat ttctgaaatt
      301 cagaaaaaaa gttccgttgg acagaggact ttttccccga aaagatgccg cgaaagatgt
      361 aggctgtgct tctcttcctg ttccatcact gtagcgtaca ggtaccatca ggttgtaaaa
      421 attatttcca aggtacagag aatacctcct gcttgtatcc agaaaaaaag tcagtcctac
      481 tcctccgtaa tccttcgcat tcagacttgg atcagcgcga aatccgctct tcatcatatt
      541 gtccgaactg aaattgtact tcagattaaa tatcaggtat ctgaaaccta cctgcatggc
      601 aactcttgct ccctcactcg ctccctcatt cagaatggct ctgtttgaat tgtaaacccc
      661 ggtctgaaac tgaatatttt ttgtgggtgc aatatttgta tgcagttcaa acatatttct
      721 tctgtcagta tccttcgtca gcatctgatc ctccctggcc agatactgtg caggtgaggc
      781 catattcaca ttgttggtat aataatttgc tgtccttgat tcacctgtca taaatcctgc
      841 actcactatc ttgttgtttc caaagcttaa gccggccatt gtagtcggaa gtatctgaaa
      901 cctgtcgtcc ccgtaattgt catttgctcc ggatctgacg ttggccgtga cgtttgcgga
      961 aagtctgtac gaaagttcca gcagttttaa atcagcctca agccttggaa ctggatgata
     1021 cattccgtac tgtttctgac cggcaatttc cttatgaaga aatctggatt cactgtcagt
     1081 cactgcagaa aatgtaatat cattgtcttt ataatatgta tatggattag aggaggaatt
     1141 cagaagactg tcatttctcc agaataaatt cctgatttcg aagtttcttt ctgaatcagg
     1201 agtatatgct ccctgaaaaa taatcctaag ctcagattcc tttctgtaga gtacgggata
     1261 aattcggaaa tattcccttt ctgccctctg agaatcaggg gaagcaggga agaaaggctg
     1321 aagtcgataa accgggactg aggatgctga tttgacagct ctttctgaaa caggagcagt
     1381 tttccctctc ttctttttca tgggagtcag tgcaagtaca attgtaaacg cggtaccgaa
     1441 tgctattgta agtacaattg aacgcctgat attaaagggg gacgccatta ctctccttct
     1501 ttggtttttt tctccagctt atcttctatt agacgaagaa gacttccttt tgtatacttg
     1561 gaatcttttt ctttcctgcc cagttccatt ccaaaaacct ccggaatgat atcgcttaca
     1621 tgggaaaaag tgaatatatt gaatttaccc tttttaactg cgtctctgat ttccggtttt
     1681 agtacaagat ccttggaatt agtttcagga ataaatacct tatagctgtc tttggaagag
     1741 cctaatatcc tgctgatatc aaaccagccc tccagtttaa gtgcaactgc acccactgcc
     1801 agaatatcgc cgtattgaga aatggaacct gtcagtgcaa tatttgcagg aatttccact
     1861 ccggaaagag cagagatgac tgctat
//
LOCUS       DAOQGJ010000796         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43033_length_1884_cov_3.680700, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000796 DAOQGJ010000000
VERSION     DAOQGJ010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43033_length_1884_cov_3.680700"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1110
                     /locus_tag="PK683_12315"
     CDS             175..1110
                     /locus_tag="PK683_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH09277.1"
                     /translation="MENIPRVSVKNLSRCFGSVKAVSSLTLSLNEGEIYGLIGPDGAG
                     KTTSLRMITGVLENEEGEVTVNGIDVKRFPERARENLGYMPQAYGLYSDLTVYENMKF
                     FSMLLGITGKKFQSRSDEILNFVKLNSFRDRKAGALSGGMYKKLAIACSIIHSPKVLI
                     LDEPTNGVDPVSRRDLWSLFFSLSEQKVSILVSTPYMDEAERCHRTGLIFHGKLLKEG
                     TPESLMQNLKNRILSVKSDSSQEMMKFLKSSVPSLNCYHFGTEIHLILPSEREGAEKI
                     KNFIIEKSNIQNCQVRNAAPSFEDVFMESSGEPSE"
     gene            1103..>1884
                     /locus_tag="PK683_12320"
     CDS             1103..>1884
                     /locus_tag="PK683_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH09278.1"
                     /translation="MSNAVEVSSLIKRYGDFYAVNNISFTVEKGEIFGFLGANGAGKS
                     TTIKILCGITSPSSGKASVAGFDAAEQSARIKEKIGYMSQKFTLYDDLTVEENIRFFA
                     GIRNIAGETLNTKMKEVLELSELEGRGKLITKDLPGGWKQKLALGCSILHGPEILFLD
                     EPTAGVDPQARRVFWEIIFSLQKKGTTVFITSHYMDEVEQCSRIALMHRGSIAALKSP
                     EDLKKDIIPGTILNLQCRNPLAASPQLQKIPEIRRIDPLGRG"
BASE COUNT          608 a          374 c          436 g          466 t
ORIGIN      
        1 ccgttggaac agtcactcat atttcagaca gagcagagtt caccccgaag aatgttcaga
       61 ctgctgatgc ccgatccaga cttgtttttg cactcaagat aagtctggac aatcctaaga
      121 aaatactgaa accgggaatg cccgccgatg taaaaatttc atcagcagaa tgatatggaa
      181 aacattccaa gagtttcagt aaaaaatctt tcaaggtgtt tcggctcagt aaaagctgtt
      241 tcctcgctga ctctttctct gaatgaggga gaaatttacg gactgatcgg gcctgacggt
      301 gcaggaaaga caacgtctct tcggatgatc accggagtcc tggaaaacga agaaggagag
      361 gtcactgtaa acggaataga cgtaaaaagg tttccagaga gagcgagaga aaaccttgga
      421 tatatgccgc aggcatacgg actgtacagt gatcttactg tatatgaaaa tatgaaattt
      481 ttcagcatgc ttctgggaat tactggaaaa aaatttcagt ccaggtcgga tgagattctg
      541 aattttgtca aactgaactc tttcagagac agaaaggctg gagcactttc aggaggaatg
      601 tacaagaaac ttgccattgc ctgttcaatc attcattctc caaaagttct aatactggat
      661 gaaccgacta acggagtgga tcctgtatcg agaagagatc tatggtcgct ttttttcagt
      721 ctttcagagc agaaagtatc catccttgtc agcacacctt atatggatga ggcggagaga
      781 tgtcacagaa ccggtctgat ctttcacgga aaactgctga aagaaggaac tcccgaaagc
      841 ctgatgcaga atctgaagaa cagaatactt tcagtcaaat cagacagcag tcaggaaatg
      901 atgaaatttc taaaaagctc agttcccagt ctgaactgct atcattttgg aaccgaaatt
      961 catcttatcc ttccttccga aagagaaggc gcagaaaaaa taaaaaactt tataatagaa
     1021 aaatcaaata ttcagaactg ccaggtcaga aacgcagccc cttcgtttga agatgtgttt
     1081 atggaatctt caggagaacc cagtgagtaa tgcagtagaa gtaagttcac ttatcaaaag
     1141 gtacggagat ttttatgcag taaacaatat ttcgtttact gtagaaaaag gagagatttt
     1201 cggattcctg ggcgcgaacg gagcaggcaa atccactaca attaaaattc tctgcggcat
     1261 cacttccccg agttccggaa aagcctcggt cgcaggtttt gatgcagcag aacagtctgc
     1321 caggatcaag gaaaaaatcg gatatatgtc tcagaaattc actctgtatg atgatctcac
     1381 tgtagaagaa aacatccgtt tttttgcagg gatacggaat attgcaggag agacgctcaa
     1441 tacgaaaatg aaggaggtgc ttgaactttc ggaacttgag ggcaggggaa aactgatcac
     1501 caaggacctt ccaggaggct ggaaacagaa gcttgcactc gggtgttcca tccttcacgg
     1561 accggagatt ctttttctgg atgaaccgac tgcaggagtt gatcctcagg cgagaagagt
     1621 tttctgggaa attatatttt cattacagaa gaaaggaaca acagtcttta ttacctcaca
     1681 ttatatggat gaagtggaac agtgttcaag aatagccctg atgcacagag gcagtattgc
     1741 agcgctgaaa agtcctgagg atctgaagaa agacattatc cccggaacaa ttctgaatct
     1801 tcagtgcaga aacccgcttg cagcatcacc gcagcttcag aaaatcccgg agatcagaag
     1861 aattgatccg ctaggaagag gttt
//
LOCUS       DAOQGJ010000797         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43037_length_1884_cov_3.510662, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000797 DAOQGJ010000000
VERSION     DAOQGJ010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43037_length_1884_cov_3.510662"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PK683_12325"
     CDS             <1..447
                     /locus_tag="PK683_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH09279.1"
                     /translation="VWLISDLPESDLNRLIEGINCDIFFPSFPKETFKGKVEAIAEVI
                     NSETRKARIRIYLHDAQDKLRPGMYGKVRVSMPENGLLIPKAALFSSNGKYYIFIKKN
                     DNIFERREVQISSEHEGLIEIAEGLEENEDTVISNVFLLKGLSFGI"
     gene            448..>1884
                     /locus_tag="PK683_12330"
     CDS             448..>1884
                     /locus_tag="PK683_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH09280.1"
                     /translation="MNLFTYFQNRPSLGVLVMTVVTILGILSWNGDKVTAYPDLSDTD
                     ATVIVRAPGLSAEEVEMQITIPIERAMDSISGVISKRSRSGYGLAVIRLIFEEDANIL
                     DMRGLVFEQIEKLKLPAGSDYALGPLTSSIGEIYKYVLDAPDNVSLTEQREIQDYIII
                     PALLKIRGIVEVSNFGGLMRQYQVIVNPVQLEKYGISTANIEEAIRSNNENADGGNMS
                     IGSSQLNITSAGRITKKEDIENIIVENRKGIPILIKDIASVELGEYPPTGLLGYYDRT
                     DDKDVSNGIQGTVLLRKFEYAHATIAELEKLISELNRELPKGVKIRKMYSRDDYLKSV
                     RMTFYRNFILSQFVLYALVFLFFRAWKKIVLFSFSVLFSYCFYLLMCNLPSLTVDLLS
                     LQTFPPALCILPSLVFILSGKKEYHHSRNEMILLGVIFFAACFFMFQFDRIEGHLFDH
                     FGKVYAILLLGSLLYGFFILPSLSGLKFF"
BASE COUNT          559 a          386 c          433 g          506 t
ORIGIN      
        1 gtctggctga tttcggatct gccagaatcg gatctgaaca gactgataga aggaatcaac
       61 tgcgatatct ttttcccaag tttcccgaaa gagacgttca agggaaaagt cgaggcaatt
      121 gctgaagtca tcaactctga aacgagaaag gcaaggatca gaatatatct tcatgatgcg
      181 caggacaaac ttcgtccggg gatgtacggc aaagtcaggg tatcaatgcc cgaaaacgga
      241 ctactgattc caaaggccgc tcttttcagt tcaaacggga aatactatat tttcattaaa
      301 aaaaatgata atatattcga aagaagggaa gtgcagattt cgtccgaaca tgagggactg
      361 attgaaattg cagaaggact cgaggaaaac gaggacacag tgatctcgaa cgtattcctt
      421 ctgaagggac tttctttcgg tatctgaatg aatctgttta cctattttca gaacaggcct
      481 tctctgggcg tgctggtaat gactgtagtc actatactgg gcattctttc ctggaacgga
      541 gacaaggtga ctgcatatcc tgatctgagc gacaccgatg cgacagtcat cgtgagagcg
      601 cccgggcttt cagcagaaga agtggaaatg cagattacaa ttccgattga aagagcaatg
      661 gactcgatct caggagtaat atccaaaagg tccagaagcg ggtacggact ggcagtaatc
      721 cggctcatat ttgaagaaga tgcaaacatt ctggatatga gaggtctggt attcgaacag
      781 attgaaaaac tgaaacttcc tgcaggctcc gactatgcgc tgggaccgct gacttcctcc
      841 attggagaaa tatacaaata tgtactggat gctcccgata atgtatctct gacagagcag
      901 agagaaattc aggactacat cataattcct gcccttctga aaatcagggg aattgtggaa
      961 gtatcgaact tcggggggct gatgcgtcag taccaggtga ttgtaaatcc tgttcagctg
     1021 gaaaaatacg gaatcagcac cgccaatatt gaagaagcga tccgatccaa caatgaaaat
     1081 gcggacggcg ggaatatgag cataggttct tctcagctga acatcacaag cgcagggaga
     1141 atcacaaaga aggaagacat tgaaaatatc attgtagaaa accgaaaagg aataccgatc
     1201 ctcatcaagg atattgcatc ggttgaactg ggagaatacc ctcctaccgg acttctggga
     1261 tactacgaca gaacggacga caaggatgtc agcaacggaa tacagggaac agtgcttctc
     1321 cgaaaatttg aatatgcaca tgccacaatt gcggaacttg aaaagctcat atccgaactg
     1381 aacagggaac ttccgaaagg agtcaagatc agaaaaatgt acagcaggga cgactatctg
     1441 aagtcagtca gaatgacttt ttatagaaac tttatactgt cacagtttgt actgtatgct
     1501 ctcgtatttc tctttttcag ggcatggaaa aagattgtgc tgttttcatt ctcagtgctt
     1561 ttttcctact gcttttatct gctgatgtgc aatctgccca gtctgacagt tgatcttctt
     1621 tctcttcaga catttccgcc tgcgctctgc attctcccct ctcttgtatt cattctttca
     1681 gggaaaaaag aatatcatca ttccagaaat gaaatgatcc tgctcggagt gatttttttt
     1741 gcggcttgtt ttttcatgtt tcagtttgac agaatagaag gacatctgtt tgatcatttt
     1801 ggaaaagtgt atgcaatact cctgctgggt tctcttctgt atggattctt cattctcccc
     1861 tccctttcag gtctgaaatt tttt
//
LOCUS       DAOQGJ010000798         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43113_length_1882_cov_3.552271, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000798 DAOQGJ010000000
VERSION     DAOQGJ010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43113_length_1882_cov_3.552271"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="PK683_12335"
     CDS             <1..1453
                     /locus_tag="PK683_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH09281.1"
                     /translation="FFEKNKAAFLIINPADGKINDANSSAANYYGYSIEKLKTMNISD
                     INILTPPEIKKEMDTAVKMHRNYFNFRHKLSSGTIRDVEVYSTPIKIAGSDFLFSLVH
                     DVTERKMAVNALRESETRLTLAIEGAKEGTWDWHIQTGKSVFSRYWAEMLGYSLEEIE
                     QDVQSWRRLLHPEDRPRVMEILERHLAGDTEYYEAEQRMLTKSGKWKWILAHGKVIEF
                     DEKGQPIRAIGTHVDIDVYKTAHEELKLLSEKLETMNAAKDKFFSIVAHDLKGPLGNL
                     NAFLTYLSGSDDLSKEELFENLRMLEDSSKTVYRFLENLLMWARSQNGSIIYNPEFYN
                     LAEIIKTNITLLSGIADSKKITLKNKLENSLTAAFDRNMIDTVLRNLMSNSLKYTDEG
                     GSVTVSAEEKDGKIEVSIQDSGIGMDRQTSDNLFRIDVKHFSKDGTKGEKGTGLGLIL
                     CKEFIDRHGGKIWAESEPGMGSTFRFTLPKNPI"
     gene            1564..1818
                     /locus_tag="PK683_12340"
     CDS             1564..1818
                     /locus_tag="PK683_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNH09282.1"
                     /translation="MYISEKKYWYLERVTYLLGGIFNLIGILLTVFVHPYWIILPVLV
                     GLNLVVYSVTGFCIMANLLYYIGVKPLCEIKKAADNSLQA"
BASE COUNT          636 a          363 c          411 g          472 t
ORIGIN      
        1 tttctttgaa aaaaacaaag ccgcatttct aatcatcaat cctgctgacg gaaaaatcaa
       61 tgatgcaaat tcctccgctg ccaattacta cggatactca atagagaaac tgaagacaat
      121 gaatatatcc gatatcaata tactgacacc cccggagatc aaaaaagaaa tggatacagc
      181 agtaaaaatg cacagaaact acttcaattt caggcataag ctgagttcag gaacaataag
      241 ggatgttgaa gtatattcca ctcccataaa aatagcaggc agcgactttt tattcagtct
      301 ggtacatgat gtcacggaaa gaaaaatggc agtcaatgca ttgcgggaaa gtgaaaccag
      361 actcacgctg gcgatagagg gagccaagga aggcacctgg gactggcata ttcagacagg
      421 caaatcggtc ttcagcaggt actgggcaga aatgcttgga tacagtctgg aagaaataga
      481 gcaggacgtt caaagctgga gaagacttct tcatcctgaa gacagaccta gagttatgga
      541 gattctggaa agacatttgg ccggagatac agagtactat gaggctgaac agagaatgct
      601 gactaaatca ggaaaatgga aatggatact cgctcatggc aaagtcattg aatttgatga
      661 aaaaggacaa ccgatccgtg caattggaac tcatgtagat attgatgtat ataagacggc
      721 acacgaagaa ctgaagcttc tgtcggaaaa gctggagaca atgaatgcag caaaggataa
      781 atttttctcc attgtagctc atgatctgaa gggaccttta ggaaatctga atgcattttt
      841 aacgtacctt tcaggcagtg acgatctttc caaggaagaa ctttttgaaa atctcaggat
      901 gctggaggac tcttctaaga cggtctaccg cttccttgaa aatcttctta tgtgggcaag
      961 atcacaaaat ggaagtatta tatataatcc agaattttat aacttagctg aaatcataaa
     1021 aactaatatc actcttctct caggaattgc tgacagcaaa aaaataacat tgaagaacaa
     1081 actggaaaac agtctcactg cagcatttga caggaatatg atcgatacag ttttgagaaa
     1141 cctgatgagc aattcactga agtatacaga tgagggcgga tctgtcaccg tatctgcaga
     1201 agaaaaggac ggaaaaattg aagtcagcat acaggattca ggaattggaa tggaccggca
     1261 gacctcggac aatctgttca gaatagatgt caaacatttc agcaaggatg gaacaaaagg
     1321 agaaaagggt actggactgg gactgattct gtgcaaggag tttattgaca gacacggagg
     1381 aaaaatctgg gctgaatcgg aacctggaat ggggagcaca ttcagattta ctcttcctaa
     1441 aaacccaatt tgagattctg acgtcaccgt ttcagccgga tctttcagac tgagcgcaat
     1501 tttctcttga cggatcatat accataccca ctatggtata atactgactg aaggaaaaaa
     1561 ctgatgtata tttctgaaaa aaaatactgg tatctggaaa gggtcactta tcttctggga
     1621 ggcatattca atctcatcgg aatacttctt acggttttcg ttcatccata ctggatcatc
     1681 cttcctgtcc tcgtaggact gaatctcgta gtctactctg tcactggatt ctgcattatg
     1741 gccaacctgc tttactatat tggagtcaaa cctctctgtg aaataaaaaa agctgcagac
     1801 aattccctgc aggcataaaa gcattctgac tgcaggtatt cgctgttttc tgaaatgcgg
     1861 aaaacagcaa gcacttcttc cc
//
LOCUS       DAOQGJ010000799         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43228_length_1879_cov_3.271930, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000799 DAOQGJ010000000
VERSION     DAOQGJ010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43228_length_1879_cov_3.271930"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK683_12345"
     CDS             complement(<1..264)
                     /locus_tag="PK683_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNH09283.1"
                     /translation="MIFNSIDFLLFFPAVYLIYLKLPHRGQNFFLLAASYFFYGYWDY
                     RFLSLLIISTIVDYSASLGIKRSESPGRKKLFLFFSIFSNLSIL"
     gene            complement(287..643)
                     /locus_tag="PK683_12350"
     CDS             complement(287..643)
                     /locus_tag="PK683_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLU-1-like domain protein"
                     /protein_id="HNH09284.1"
                     /translation="MEFPELEPYFQAITDITDNIASINTHYDVDHEKDFSELEKIAVD
                     VFSRSWEKTDENYFKLFTSYYTFHIKIIEELISEARRILNPEKREYLKRLVAYKKSSD
                     EWFAALKKKRKLAAVA"
     gene            complement(733..1074)
                     /locus_tag="PK683_12355"
     CDS             complement(733..1074)
                     /locus_tag="PK683_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH09285.1"
                     /translation="MKSGKKIAVTYSNCPVTYCMDKIGGKWKPVIIYLIRKGANRFGI
                     LHRGIEGISRQMLTQQLRELEEDRILNREVFPEVPPRVEYSFTDYGKSLFPIIDAMIK
                     WGNKNMEIFSD"
     gene            1593..>1879
                     /locus_tag="PK683_12360"
     CDS             1593..>1879
                     /locus_tag="PK683_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09286.1"
                     /translation="MKKLIVFLIIIISVSCAADRKRKIDAEAAHITEKMAVSLNLSPE
                     QKARVLSINKKRAEGHYAAYDAGRKKDIITAAVNTWETELKTVLTPEQADKL"
BASE COUNT          587 a          370 c          355 g          567 t
ORIGIN      
        1 taagatgctc agattggaaa aaatgctgaa gaaaaggaag agctttttcc tgcctggact
       61 ttctgatctt ttgatgccaa gcgaggcaga ataatctacg attgtggaaa ttataagaag
      121 tgaaagaaat ctgtaatccc agtatccgta aaaaaaataa ctggcggcaa gcaggaaaaa
      181 attctgtccc ctgtgaggca gtttcagata gatgagataa actgccggaa aaaaaaggag
      241 aaagtcaatc gaattgaaaa tcattcaact tcctccagaa gttatattat gctactgcgg
      301 ccagtttgcg ttttttcttg agagcagcaa accattcgtc ggaacttttc ttatatgcca
      361 caagtctttt caaatactct cttttttcag gattcagaat tcggcgggct tcgctgataa
      421 gttcctctat gatttttata tgaaaagtgt aataacttgt aaataatttg aagtaattct
      481 catctgtttt ttcccaggat ctggaaaaaa catctacagc aatcttttcg agttcgctga
      541 aatccttctc atggtctaca tcatagtgag tattgatgct tgctatatta tctgtaatgt
      601 ccgttatagc ctgaaaataa ggttccaatt ccggaaattc caattctagc tccttttacg
      661 gattttttac catcttacac cctataggtg attttgacaa gaaatttgag ccggatcaaa
      721 tcggtaatcc tgtcaatctg agaaaatctc catatttttg ttcccccatt ttatcattgc
      781 atctataatt gggaaaagag attttccgta atccgtgaaa ctgtactcca ctctaggggg
      841 gacttcggga aaaacctctc tattcagaat tctgtcctct tcgagttcgc gaagctgctg
      901 cgtaagcatc tgtctgctga tgccttctat cccccgatga agaattccga aacggtttgc
      961 tcctttccgg atcagataga taatcacagg tttccatttt cctccgatct tatccataca
     1021 gtaagtcacc ggacagttgg aataagtgac tgcaattttt ttaccggact tcattgaacc
     1081 tcctcagacg aaatataata tatatattta ttcaggtttg tcaagcagca ttgctgaaaa
     1141 cattacaatg tatctgcagc atacactcac tgaactgttt caggcagaag acggatgcaa
     1201 tcagggaata ttcggcgcct tcaaagcaga atgagctttt tcatcaggat aatatatgcg
     1261 cagatatact cattttcata aataattgtg ctttttccgg ctgacagact gatttcactg
     1321 agtcgtgaac aaataaatac tcaaagattt ttgaatactg gcataaaggc atccgtaatc
     1381 tttcagatcc ggcaatcagg aaaaaatctg aatatgaaag tgcttccgtt caatcttcct
     1441 gccgcaatat ccggataaga acaggttttc ccggccaaat attggaaaaa tttttcagaa
     1501 ataaaaattt tttccaatag tcttgttttc atgactactt gactttttaa gactataaag
     1561 ctattattct gcttgtacaa aaggagagtt ttatgaagaa attgatagta tttctaatta
     1621 taattatttc tgtatcctgt gcagcagaca gaaagcggaa aattgatgca gaagcagccc
     1681 atatcacgga aaagatggcc gtcagtctga atctgagtcc tgaacagaaa gcccgtgtcc
     1741 tttcaatcaa caaaaagaga gcagaaggac attatgcggc atacgatgct ggaaggaaga
     1801 aggacatcat cactgcagca gtaaacactt gggaaactga acttaagaca gttctcaccc
     1861 ctgagcaggc cgataaact
//
LOCUS       DAOQGJ010000800         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43279_length_1878_cov_2.675261, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000800 DAOQGJ010000000
VERSION     DAOQGJ010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43279_length_1878_cov_2.675261"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..540
                     /locus_tag="PK683_12365"
     CDS             328..540
                     /locus_tag="PK683_12365"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNH09287.1"
                     /translation="MTAAEHLFKISQELPDDLLTELLDFAEFLKQKKKLPAQTNLALK
                     IQQQFKDLDFDNVSLPARSVMRNTPF"
     gene            987..1688
                     /locus_tag="PK683_12370"
     CDS             987..1688
                     /locus_tag="PK683_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09288.1"
                     /translation="MFPDLHLQQNALYFFISLKKYWRKTNEIRICSNSKQQTANSKQQ
                     TATKLISITALLAFGLIGCTTKKKDNSKALIGLLALNSMKAAATCTTANYISSPITSS
                     TFTDLKFSYISAYTAWLGYFSITGATSTSWWLVEGPATASISSASFTTYLTDQGCSGE
                     TSANSNAAPAMYGVQKDQVNNVPATFPIATSSISANQKYYSPTTGATILFRMFISDST
                     ASSSVSTGWKVKKAY"
BASE COUNT          610 a          398 c          358 g          512 t
ORIGIN      
        1 gatcgtatgg atttgtaact actcagccta gcggcttcgc agttacaaat accctttttc
       61 tagaaattgc ttttaaaaaa gcaagaaaag gttggtccct atcgggagga tttaggggtt
      121 tgcaacccct aagaccccgc ctttcaagct tttttctata aattctctta caatgtattt
      181 tttcactgag tagttacatg gattttagca atttggcaca acagcagaaa accggaaggc
      241 tggaaagaaa gagtcaacga tctggattga aatgaattga cttttcggtc ggatgagcca
      301 tactgtctgg aaaaggagaa tgcagtaatg acagcagctg aacatctttt taaaatcagt
      361 caggagcttc cggatgatct gcttacagaa ttattggatt ttgcagaatt tctcaagcag
      421 aaaaaaaagc ttcccgcaca gactaatctt gcactcaaaa tacagcagca attcaaagat
      481 ttagattttg acaatgtctc actgcccgca agaagcgtaa tgagaaatac acctttttag
      541 agtcattata atgaatagtt atttattaga taccaacgta ctctcagaat tgatgcggtc
      601 taatccagat aggactgttt ttgaatggtt tgagtcaaat aaaaattcca attttttcat
      661 ttcttcagta acggaagctg aaattttgac aggaattgct ctcctcccgg aaagtaaaaa
      721 gcgactggac ctgactgccg cttctgaaaa gcttttttct caggattttt ttggacgaat
      781 actgtttttc gatgaaaaat cagcagtcat ttatgcgaag actgccgccg atagaatcaa
      841 atctcctatt ttcccgaatc cggttaaaaa ccaaacgatt tttctccgta aaaaaaatct
      901 ggacaaaatg aacgatatgc cataaatata caattatcat ttaaggaaca aaacagtgta
      961 atgcctttaa gaaaccggca gcaagaatgt ttccagactt gcaccttcag cagaatgcat
     1021 tatatttttt tatatcctta aaaaaatact ggagaaaaac caatgaaatc aggatctgtt
     1081 caaacagcaa acagcaaaca gcaaacagca aacagcaaac agcaactaag ctaataagca
     1141 ttactgccct tctggcattc ggcctcatcg gatgcacgac taagaaaaaa gacaattcga
     1201 aagcactgat aggacttctt gcactgaatt cgatgaaggc tgcggctacc tgcacgacag
     1261 caaactacat ctcgtctccg atcacatcaa gtacatttac tgatctcaaa ttcagctaca
     1321 tctctgcata tactgcatgg cttggctatt tcagcataac cggggcaacc agcactagct
     1381 ggtggcttgt agaaggcccg gcaacagctt ccatttcctc agcatccttt actacatatt
     1441 tgacagacca gggctgttcc ggtgaaacaa gtgccaattc caatgctgcc ccagcaatgt
     1501 atggagttca aaaagatcag gttaacaatg ttcctgcaac tttccctatt gcaacttcat
     1561 caatctcagc gaatcagaaa tattattcgc ctacaaccgg tgccactatc cttttcagaa
     1621 tgtttatatc tgattcaact gcctcgtctt cagtatcgac tggatggaaa gtcaagaagg
     1681 cgtattgaaa ttcgagccgc tgtaaagaag aagtaaataa tctaaaaagc ggaaagcagc
     1741 atcagtgaaa ggtcaattct agcggacact atatgtcgca gaaaaggcta ggtaaagcgg
     1801 tgatagtagc taccaagttt gcagcagctt ccactcttgc gacccccttt gggcttatgc
     1861 tgtcaactta agaagaaa
//
LOCUS       DAOQGJ010000801         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43280_length_1878_cov_2.662095, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000801 DAOQGJ010000000
VERSION     DAOQGJ010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43280_length_1878_cov_2.662095"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1497)
                     /locus_tag="PK683_12375"
     CDS             complement(103..1497)
                     /locus_tag="PK683_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09289.1"
                     /translation="MNEGISKILYKINDKEYRENSFDLKGKPVSETHFKILPDGWEIK
                     KISGKDIRRHLYDGRGIEVQSEKFESNGTLNERTEYKYDEHCLNTNRFFSCRTVTEIY
                     GADGKLKNDYSGAARTVNKYDQNCLNKTDPAGLELIELNCRALMDEYSSEGKRLRARL
                     FTYDDKGNFLYGEEIGTDGKIINRLQPFFYKFDEKGNLIQKETGREMAKEIGYMRQVY
                     EYDQNCLNTGKPSSHCISLEETYDADGKPKELGFFSSEPFRMVYKYDMKGNKILQESY
                     GADGRLIQAINGNIIARKVFKYDEKGNLIEVENYGSDEKLVTADPAAARTVYKYDNNC
                     ILAGNQSKDCKNLEEFYGADGKLTVSFFNLSARIIYNFDENGNRTKIEYFGPDGKLKD
                     NDEGFARKISKYDSNGNNLSSRYINVSGSVVRMELFQIFPHPFLSGKILKLKLDMDRF
                     FRMEDVIQEASKIK"
     gene            complement(1677..>1878)
                     /locus_tag="PK683_12380"
     CDS             complement(1677..>1878)
                     /locus_tag="PK683_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09290.1"
                     /translation="FMKTISIDDHLELKEIQISDAPEMFQTIDNERASLRTWLPFVDF
                     TTEKKKRKLFITVKKKPRSSPK"
BASE COUNT          482 a          395 c          286 g          715 t
ORIGIN      
        1 ttcagtttta tcataacaca tattcgactg cccggtccgg cattctgtat acaagaatga
       61 atcttccgca ttgttcctga gcttttagac tatcccttct tttcatttaa tttttgatgc
      121 ctcttgaatg acatcttcca ttcgaaagaa tctatccata tccagtttca actttaatat
      181 tttacctgat aggaatgggt gaggaaatat ttggaagagt tccattctga caacagatcc
      241 agatacattg atgtaccggg aagataaatt atttccattg gaatcgtact tggaaatttt
      301 tcttgcaaac ccttcgtcat tgtccttcag ttttccatcg ggtccaaagt attctatttt
      361 cgtccggttt ccgttctcat caaaattata aataatacgt gctgaaaggt taaaaaaaga
      421 cactgtaagt ttaccatctg ctccataaaa ctcttccaaa tttttacagt cctttgactg
      481 atttcccgcc agaatacaat tattatcata tttatatact gttcgggcag cagcggggtc
      541 tgcagttaca agcttttcgt cagatccata attttctact tcgatgagat ttcctttctc
      601 atcatacttg aatacttttc gagcaattat attgccattt atagcttgaa taagccggcc
      661 atcggctcca tacgattcct gtaagatttt atttcctttc atatcatatt tatataccat
      721 acggaaaggt tctgagctga aaaaccccag ctctttcggt tttccatccg catcatatgt
      781 ttcttccagc gatatgcaat gactggatgg ttttcctgta ttcaggcaat tttgatcata
      841 ctcgtatact tggcgcatat agccaatttc ttttgccatt tcacgacctg tctctttctg
      901 tatcaggttt cctttttcat caaatttata gaaaaatggc tgcaatctgt ttatgatctt
      961 tccgtcagtg ccaatttctt ctccataaag aaagtttccc ttatcatcat atgtaaacaa
     1021 tcgtgctctt aagcgcttcc cttcagagct gtattcatcc atgagagccc tgcagttcag
     1081 ttcaattaat tctaatcctg ctgggtcagt tttgtttagg caattctgat catacttgtt
     1141 tacggtacgg gcagcacctg aataatcgtt cttcagtttg ccgtcagctc cataaatttc
     1201 cgtcacagtc ctgcaggaaa aaaatctatt tgtatttaag caatgttcat catatttata
     1261 ttctgtcctt tcattgagtg tgccgtttga ttcaaatttt tctgattgta cttcaatgcc
     1321 tctcccatca tataaatgcc ttctaatatc tttcccgctt atttttttaa tttcccatcc
     1381 gtctggaagt atcttaaaat gggtctcact taccggtttg cctttcagat caaaagaatt
     1441 ctccctgtat tctttatcat tgattttata aagtatttta ctgattcctt cattcatact
     1501 tggtaaatat ttttcattct cctttttcag gaactgtaac tctaaaagtc tgtcgtattt
     1561 gtccctaacg atttttgcca gcagaccatt tgaatccccg cttctgccga aagaaacaat
     1621 ttcgcttttt aaattttttt gagattttga tgctctggtt tgagaatttt cctcatttac
     1681 tttggagatg accttggctt cttttttact gtgatgaata gttttctttt tttcttttct
     1741 gtagtaaaat caacaaaagg gagccaagtc cgaagactgg ctctctcatt atctattgtt
     1801 tgaaacattt caggtgcatc agagatttga atctctttta gttcaagatg atcatctatc
     1861 gaaattgttt tcataaac
//
LOCUS       DAOQGJ010000802         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43288_length_1878_cov_2.274273, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000802 DAOQGJ010000000
VERSION     DAOQGJ010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43288_length_1878_cov_2.274273"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PK683_12385"
     CDS             <1..669
                     /locus_tag="PK683_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09291.1"
                     /translation="EWVLKYKYDPSKKPLEKNNEAYKKIKEKHSKEKVYFMHDNGGRP
                     FVVYLSKEKISVYREVQNKFYVREKDRVWDSDRKNAWFYIEHVVTKKNFEKVWVGKSI
                     SNDMTKWVKTDTKEFLGNSILVKLNASRYLFIGESVFEFTPNEEIQTFYSPVGNNDVP
                     YPYAIGEEYVYFMIERKRLPISEFKEFTEYVKKNAYNEFYENDLEEKVSSFPKIKEIQ
                     SRLF"
     gene            812..1117
                     /locus_tag="PK683_12390"
     CDS             812..1117
                     /locus_tag="PK683_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09292.1"
                     /translation="MNRWIVIAVLFSAVFSVSANGRHGGHPHHGNHHPNRNGHHQVQP
                     NQPKHGSEYSQFGNGQNGIGRPGNGIGTPGTGYGNPGNGTGTPGNDYGNPGNPDLRP"
     gene            1425..>1878
                     /locus_tag="PK683_12395"
     CDS             1425..>1878
                     /locus_tag="PK683_12395"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNH09293.1"
                     /translation="MSSKKISVIITVFLTLFLFHCPSKDNGADKTKLAVAAAVVTAPK
                     PAYVFNSLTYSSTLTKGSKGYFAVPIGTSFTLTASAKNTNTGSSTPSGYTVKYSVKPD
                     LPSGLTLDTATGNITGVYSAAPSSKTEAYTVTGTFTAQSGYAISGESTV"
BASE COUNT          626 a          361 c          373 g          518 t
ORIGIN      
        1 gaatgggttt taaagtacaa gtacgatccg tcaaaaaaac ctctggaaaa gaacaatgag
       61 gcctataaga aaattaagga aaaacactcc aaagaaaaag tgtattttat gcatgacaac
      121 ggcggaaggc cttttgtcgt ctatctttcc aaagaaaaaa tctccgtgta ccgtgaggtt
      181 cagaataagt tttatgtcag agaaaaagac cgagtctggg acagtgaccg taagaatgca
      241 tggttttata ttgaacatgt ggttacaaag aaaaactttg aaaaagtgtg ggtcggaaaa
      301 agcatatcga atgatatgac aaagtgggtt aaaactgata ccaaagaatt tctcggcaat
      361 tctatattag taaaacttaa tgcttcccgt tatcttttta tcggtgaatc tgtatttgaa
      421 ttcactccga atgaggagat tcagactttc tactcacctg taggaaacaa tgatgtcccc
      481 tacccttatg caatcggtga agagtacgtt tattttatga ttgagcgaaa acgacttcct
      541 atctcagaat tcaaagaatt tacagagtat gtgaagaaaa atgcctacaa tgaattttat
      601 gaaaacgacc tggaggaaaa agtatcctct tttccaaaga taaaagagat tcaaagtcgg
      661 cttttttagt taaggaaata tgattataac ctctttcgag attttcctga tttttagaga
      721 tcaaaacgcc tgaaaactgg attttatttc gatctgactg ttaaaataca aactgtttaa
      781 agaccgtcta atatttcagg agatcttcaa aatgaacaga tggattgtaa tagcagtgtt
      841 gttttcagca gttttctcag tttctgcaaa tggaagacac ggaggacatc ctcatcacgg
      901 gaaccatcat ccaaatcgga atgggcatca tcaggttcag ccgaatcagc ctaagcatgg
      961 atctgagtac agccagttcg ggaatggaca gaacgggata ggcagacctg ggaacggaat
     1021 cggcactcct ggcacaggtt atggaaatcc tgggaacgga accgggacac cagggaatga
     1081 ttatggaaat cccggaaatc cggacttaag accctgaacc gaaaattcgc atatccagta
     1141 cgaataaact gatgaatcat aacatatatg tcattttctt taatgcgggg accgcttcgt
     1201 caaaagcttc accatcctgc tccagtcggc cttcaacctc aaatcaataa tttttttata
     1261 cctctgccat actaaaaagg caggcttaga tcagtttcaa agtttatgag aaatcataag
     1321 tctggcagat ttttcactgt aatgtttgct gaattgctga aatatttatt gcattttaaa
     1381 aagcctgatg agatatcgaa tcgaaaactt aaattgaggt aaaaatgagt tctaaaaaaa
     1441 tttcggtcat tatcacagta tttttgacct tattcctttt ccattgccca tccaaagaca
     1501 atggtgcgga taaaacgaaa ctggctgttg cagctgcagt agtcacagca ccaaaaccgg
     1561 cgtatgtttt taattcactt acatacagtt ctacattgac aaaagggtca aagggatact
     1621 tcgcagtccc gatcggaact tcatttacgc tcactgcatc cgcaaagaat actaatactg
     1681 gatccagtac tccatcagga tatacagtga agtattcagt caaaccagat ctcccttcag
     1741 gtttgacctt ggacacagcg actggaaata ttacaggtgt ttattctgcg gctccatctt
     1801 caaaaacaga agcatacaca gtcactggca catttacagc acaaagcgga tatgcaattt
     1861 cgggagaatc caccgtat
//
LOCUS       DAOQGJ010000803         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43442_length_1874_cov_2.921385, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000803 DAOQGJ010000000
VERSION     DAOQGJ010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43442_length_1874_cov_2.921385"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PK683_12400"
     CDS             complement(<1..1334)
                     /locus_tag="PK683_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002137878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNH09294.1"
                     /translation="MRETGAQYLIRFLESKNVTCIAGMPGGSILPVYDALYGSRIQHI
                     QARHEQGACFIAGGIARASGKMGVVFATSGPGAANLLTGLADAKMDSVPLLAVTGQVP
                     RALIGTDAFQELDICSMSIPATKARFFIDSPESLARNLPIAFHTASTPRFGPVLIDIP
                     KDIQSELYEYPDLFPNPQTELRERSLPDLREWISLLENSEKPILYLGGGCIASNASEE
                     IIQLSEKNGIPAVSSLNGLGVFPAFHRNFLGMLGMHGLPFVNYALEEADLLFALGTRF
                     DDRATGKLSEFCPNAKILHIDIDRRELNRLKKADLSAAFDIKEFLKILLPHISERSRD
                     VWNFRISENRKNSFSAAETVYNYDNPSFYIRKAADCMNPYDITVTDVGQHQMWTAQHY
                     PFQKPRTFLCSGGLGTMGFGLPSAIGAALAFQDRKVLCITGDGSILLNIQELA"
     gene            complement(1578..>1874)
                     /locus_tag="PK683_12405"
     CDS             complement(1578..>1874)
                     /locus_tag="PK683_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09295.1"
                     /translation="IIACTILMFLFQATYGDSLYSAMKAGQFSSLAFFGVVIGAVFSG
                     LAEFFSGHGLGGLLEDNLLIPIVASLVLAYYASFAGLAASQELFFPIQELFTKV"
BASE COUNT          553 a          447 c          405 g          469 t
ORIGIN      
        1 gccagttcct gaatgttcag aaggattgat ccgtctccgg taatgcagag aacttttctg
       61 tcctgaaagg caagagccgc tccgatggcg gaaggcaggc cgaatcccat tgttccgagt
      121 cctccggaac atagaaaggt tctcggtttc tgaaaaggat agtgctgagc tgtccacatc
      181 tgatgctgcc cgacatccgt tacagtgata tcataaggat tcatacagtc tgctgccttt
      241 cttatataaa aagatggatt atcataatta tatacagtct ctgcagccga gaaagaattt
      301 tttctgtttt cagagattct gaaattccag acatccctcg acctttctga aatatgaggg
      361 agcagaatct tcagaaattc cttaatgtcg aaagcagcag aaagatccgc cttcttcaga
      421 cgattcaatt ctctccgatc aatatcaata tggaggattt ttgcattggg acagaactcg
      481 gataatttcc cggttgccct gtcatcaaac ctggtaccca gagcaaaaag cagatcggcc
      541 tcttccagag cgtaattgac aaaagggagg ccgtgcatac cgagcattcc cagaaagttt
      601 ctgtgaaatg caggaaatac tccaaggccg ttcagactgg atactgccgg aattccattt
      661 ttctcggaaa gctgaataat ttcctccgaa gcattggaag caatacaccc tcctccaagg
      721 tacagaatcg gcttctctga attttccaga agactgatcc attctctcag atccggcaga
      781 gacctctctc ttaattccgt ttgcggattt ggaaataaat caggatactc gtacagttct
      841 gactgaatat cctttggaat gtcaatcagt acgggaccaa atcgaggagt ggatgctgta
      901 tgaaatgcga ttggaagatt cctggcaaga ctttccggtg aatcaataaa aaaccgtgct
      961 ttcgtagccg gaatggacat actgcagata tccagttcct ggaatgcatc ggttccaatc
     1021 agggcccgag gaacctgacc tgttaccgcg agcaagggta ccgaatccat cttcgcatca
     1081 gccagtccag taagcaggtt tgcagcccca ggtcccgaag tagcaaaaac tactcccatt
     1141 ttcccagatg cccttgcaat tcctcccgca ataaaacagg ctccctgctc atgccttgcc
     1201 tgaatatgct gaattcgact gccgtacaat gcatcataca cgggaaggat tgaacctccg
     1261 ggcattcctg caatgcaggt tacattcttt gactctaaaa atcggattaa gtattgagca
     1321 ccagtttctc tcatatctac ctctcccggt gaagccggaa gatgttgaat gagtgcactc
     1381 taggtccgcc ggcagtaccg gcgattataa atttacaatt ccccggtggt tatgtaagaa
     1441 acattgacca ccacagaaag catttgaata gaatctgctt tgttatgaaa ttgcatatgc
     1501 tcagatcaaa acaactgcct gcaaagtcaa gtttttttcc gacttccgaa taactgttaa
     1561 aatctggcgg agagaaatca aaccttagta aacagttcct gaataggaaa gaaaagttcc
     1621 tgagatgcgg caagtccggc aaaacttgca taatatgcca gaacaaggga tgcaacaata
     1681 ggaatgagca gattatcttc caaaagtcct cccagtccgt gaccggagaa aaattcggct
     1741 agcccggaaa aaactgcacc gatgacaact ccaaaaaaag ccaaggatga aaactgtcct
     1801 gccttcattg cggaatacag actgtctccg taagtcgcct gaaaaaggaa catcaggatc
     1861 gtgcacgcaa tgat
//
LOCUS       DAOQGJ010000804         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43475_length_1873_cov_3.282178, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000804 DAOQGJ010000000
VERSION     DAOQGJ010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43475_length_1873_cov_3.282178"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PK683_12410"
     CDS             <1..1439
                     /locus_tag="PK683_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH09296.1"
                     /translation="NGNWRSSSEKAEIVNPANSEKTAEASNADESMIDECIRASISAF
                     NLTRKSSRFIRSRILKKIADGILKRQEELVSCISDEAGKPCQLALAETERAYQTFLWA
                     SEEVRRFGAESFPMDIDAYGRNFYDGKILRCPLGVILAVSPFNFPLNLAAHKVAPALA
                     CGASVILKPPPAAPGASVILGELFLEAVSESREESFSESALQIIHCSNQDAEKLATDE
                     RISVLSFTGSDRVGWHLKTIAGRKKVLLELGGTACTAVFKDADLNQAAERCIFGAFSY
                     SGQVCISIQKILIEDSIYDKFCREFKEKTQKLNQWRTGEKEGILGPLINRNSMERILS
                     WVQEALDGGAKLLTEIRFEGNFLSPIVMEDVPKNCRLQTEEIFGPVVIMERFSDMQDA
                     VLKINSSRYGLQNAVFTEDRQTIHFCTEEISSGSVLINEIPTYRADHMPYGGVKDSGM
                     GKEGIRYAMNEFTFEKLILEKKPSLPDC"
     gene            1496..>1873
                     /locus_tag="PK683_12415"
     CDS             1496..>1873
                     /locus_tag="PK683_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HNH09297.1"
                     /translation="MTGKKGYFISIGGGENQLPLIRAVKDSGYRLIIADRNPKAPGFK
                     EADIKIMESVTEYRRIYNYILKTLLDAPIVGIGCRSFGRASVSAAYLADKLRLNGNTV
                     NSVKFFQNKFQYKKYLQTKGIPVP"
BASE COUNT          582 a          382 c          441 g          468 t
ORIGIN      
        1 taaacgggaa ttggagatcc tcttccgaaa aagcagaaat cgtcaacccg gccaattctg
       61 agaaaactgc agaagcatca aatgctgatg aatcaatgat cgatgaatgc attcgggcct
      121 cgatttcggc atttaatctt actcggaaaa gctccagatt catcagaagc agaattctga
      181 aaaaaattgc agatggaatt ctgaaaagac aggaagaact cgtgagctgc atttctgatg
      241 aggcaggaaa accctgccag cttgcccttg cagagacaga gagagcatac cagacatttc
      301 tatgggcatc cgaggaagtc agaagattcg gagctgaatc atttcctatg gacatcgatg
      361 cctatggaag aaacttttat gacgggaaaa tactcagatg tccgctgggc gtcattctgg
      421 cagtctcacc ctttaatttt cccctgaatc tggctgcgca taaagttgcg cctgcactgg
      481 cctgcggggc atccgtgatt ttaaaaccgc ctcccgctgc tccgggagca tccgttattc
      541 tgggtgaact ttttctggag gccgtcagtg aaagcagaga ggaaagcttt tcagaatcag
      601 cactgcagat tattcactgt tcaaatcagg atgctgaaaa actggcaaca gatgaaagaa
      661 tttctgtctt aagctttact ggaagcgaca gggtcggctg gcatctgaaa acaattgccg
      721 gcaggaagaa agtgcttctc gagcttgggg gaactgcctg tacggctgta tttaaagatg
      781 cagatctgaa tcaggctgct gaaagatgta ttttcggtgc attttcctac agcggacagg
      841 tttgtatctc cattcagaaa attctgattg aagattcaat atacgataag ttctgcaggg
      901 agtttaagga aaagactcaa aaactgaatc aatggagaac aggagaaaaa gaaggaattc
      961 tcggtccatt aatcaaccga aactcaatgg aacgaattct ttcctgggtg caggaagctt
     1021 tggatggcgg tgcaaaactt ctgactgaaa tccggtttga aggaaatttt ctttctccga
     1081 tcgtgatgga agatgttcct aaaaactgca gactgcagac tgaggaaatc ttcgggcctg
     1141 tcgtcattat ggaaagattc tcggatatgc aggatgcagt gctgaaaatc aacagttcca
     1201 ggtacggact tcagaacgca gtatttacag aagacagaca gacaatacat ttctgcactg
     1261 aagaaatcag ttccggttct gttctgatca atgaaatccc tacttacaga gcagaccata
     1321 tgccttacgg aggagtaaag gattcaggaa tgggcaagga aggaatcaga tatgcaatga
     1381 atgaatttac tttcgaaaaa ctgattcttg aaaaaaaacc gagtctccct gactgctgaa
     1441 atagaagatt caatttgaca gaaacacctg atatattttt catcatactg actgtatgac
     1501 tggcaaaaag ggatatttca tttctatcgg gggaggagaa aaccagctcc ctctgattcg
     1561 ggcagtcaaa gactcaggat acagactgat tattgcagac cgtaatccga aggctccggg
     1621 attcaaggaa gcggacatta agattatgga gtccgttaca gagtacagaa gaatttacaa
     1681 ctatatcctc aagacacttc tggatgcacc aattgtcggc attggatgcc ggtctttcgg
     1741 cagagcctca gtatctgctg cttatcttgc tgacaaactg aggctgaatg ggaacactgt
     1801 aaactccgta aaattctttc agaacaagtt ccaatacaag aaatatcttc agacaaaagg
     1861 aatcccggta ccg
//
LOCUS       DAOQGJ010000805         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43482_length_1873_cov_2.875688, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000805 DAOQGJ010000000
VERSION     DAOQGJ010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43482_length_1873_cov_2.875688"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PK683_12420"
     CDS             <1..365
                     /locus_tag="PK683_12420"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HNH09298.1"
                     /translation="ESTDFEKNVSARVIDSEIHDDLDFKTVEFRIYKPEANTIALIGS
                     FNQWNPEHDYLVKQRNGLFVLRKKLKSGDYLYYYQVDGEAMVDTYNPETRFRPETEEL
                     CSYLKVPDSHKSVMAGKE"
     gene            593..>1873
                     /locus_tag="PK683_12425"
     CDS             593..>1873
                     /locus_tag="PK683_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNH09299.1"
                     /translation="MSEFRNFQFDENKNLIYHWKKNAGRYSGLKFKDRWQNAQMGFQL
                     DKEIFLNRSLDSIQFSPNSSLKIPLDKGDYEIRFSAGILEPPSAGSALSFSDGSASRF
                     LLTPGNETQKWTDYVKEIRIESGFLNISWSSSSTHIYLGRPYIRKKTVSEQPSVYLFI
                     VDSLRPDGIKALGGKLETSPNIDQLASESLIFEKHFANANWTKPSMISMFRGQYSSNL
                     GIVNPGFPVYSEEKQIFYNAKEQSITEILRKNNYYTATIMNNVFFQDYTGVGVDLGFH
                     EAVQIGKDIQDTEHITKHSLKIIEENSDRPQFLHINYNTPHGSYSPPSAVLREMISKS
                     DSKAVQSTHPILLRYYGEVRYTDLEIGKIIDYLKKINKYDSSMIIITSDHGDLFNENH
                     TFKKNEVNGTRFGHGETHYDEEIQIPLIIKLPKKM"
BASE COUNT          620 a          387 c          368 g          498 t
ORIGIN      
        1 tggaaagcac tgatttcgag aagaatgttt cagccagggt gattgacagt gagatccacg
       61 atgatctgga tttcaagaca gttgaattca gaatttacaa accggaagca aacacgattg
      121 cactgatcgg aagtttcaat cagtggaacc cggaacacga ttacctggta aagcagagaa
      181 acggactttt tgttctgaga aaaaaactga aatcaggtga ttatctttac tattaccagg
      241 tggacggtga agcgatggtg gacacataca atcctgaaac aagattcaga cctgaaacgg
      301 aagagctctg ctcctatctg aaagttccag actcccataa aagcgtgatg gcaggaaaag
      361 aataattcag ttttcattac aaaaccttaa aacaatccat ccgttgccga aattcttcag
      421 aagtccgttt tcttttttga atttccggca cactgaaaaa aaaaacaaaa actccttttt
      481 cagatttgcc cttctcgttc tttgtgccgc atttgcatgc tgcattcaga gtccacagaa
      541 taacaaagac acagaaaata aagattatta tgatttaacg agacatctga aagtgtccga
      601 atttcggaat tttcagttcg atgaaaacaa aaatcttatc tatcactgga agaaaaatgc
      661 aggccgatat tccggactga aattcaagga cagatggcag aatgctcaga tgggatttca
      721 gctggataaa gaaatattcc tgaacagatc acttgactcc attcagttca gcccgaactc
      781 ttcactgaaa attcctctgg acaaagggga ttatgaaatc cgtttttctg cgggaattct
      841 ggaacctcct tctgcagggt cagcactttc cttttcagac ggttcagctt cacgattcct
      901 cctgactcca ggaaatgaaa ctcagaaatg gactgattac gtgaaggaaa ttagaatcga
      961 atccggattt ctcaatataa gctggagttc aagttccact catatttatc tgggaagacc
     1021 ttatatccga aaaaaaacag tttcagaaca gccttccgta tacctgttta tagtggattc
     1081 actcaggccg gacggaatca aggctctggg agggaaactg gaaacaagtc ccaatattga
     1141 tcagctggct tcagaatcac tgattttcga gaaacatttt gcaaatgcaa actggacaaa
     1201 accttccatg atttccatgt ttcgcgggca gtattcttcc aacctcggga tagtcaatcc
     1261 tgggtttccc gtctatagcg aggaaaagca gattttttat aatgcaaaag aacagagtat
     1321 tactgaaata ctgagaaaaa ataattatta tactgccaca atcatgaaca atgttttttt
     1381 tcaggattat accggagtgg gtgtggatct gggatttcat gaggctgttc agatcggaaa
     1441 ggatattcag gatacggagc atataacaaa acattccctg aaaatcattg aagagaactc
     1501 agacaggcct cagtttctcc atatcaatta caatactcct cacggctctt attctccccc
     1561 gtctgccgtg ctcagagaaa tgatttccaa atcagacagc aaggctgttc aatccaccca
     1621 tccgatcctg ctccgatact acggagaagt cagatataca gatcttgaaa tcggtaaaat
     1681 tattgattat ctgaaaaaaa tcaacaaata tgacagttcg atgatcatca tcacttcgga
     1741 tcacggtgat cttttcaacg aaaatcatac attcaaaaag aatgaagtga atggaaccag
     1801 attcggtcat ggagaaactc attatgatga ggaaatccag atccccctca tcatcaaact
     1861 tccgaaaaaa atg
//
LOCUS       DAOQGJ010000806         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43486_length_1873_cov_2.756876, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000806 DAOQGJ010000000
VERSION     DAOQGJ010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43486_length_1873_cov_2.756876"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PK683_12430"
     CDS             complement(<1..640)
                     /locus_tag="PK683_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09300.1"
                     /translation="MNETDITDPILINRFNLKFNNKSFEESFKENYYWTSLSIHRFAI
                     LLAIILYALFAYLDVWMSPESKEKIWFIRFGIVVPILILTFISTIFPVFRRINQLLFA
                     FTSVSLGLGIAAMIGIAKESELGHNYYFTGLLLVIMWIYTFTRLRFISAVFSAWIVTL
                     SYFYVIIFIHDIINVSPALFLNHLFFFISSNIIGMFSVYYFEYYYRKNFIHIN"
     gene            971..1774
                     /locus_tag="PK683_12435"
     CDS             971..1774
                     /locus_tag="PK683_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH09301.1"
                     /translation="MKSILKYSFIAGAIFSIIGCTRIETGEVGLRIGFDKQVKNEELL
                     PGSFNQTIIGSVMTFPVKEVAVKIDDLTPLAKDNSTMKDFDATIIYNINQTQVAEIYN
                     SKNKSFHETHDGDTYLMYNYIFNSARNAIYKSARKYEALDMADNRAVMEQEIKETIIK
                     TLADEKLDGTITISQVLIRNITPADSIVASANELVKAKNELKTEEIKVATAKKRNESM
                     QANPTMIPLMKAEAEAQYLRELPAAIANFKGQTLIIGGGASPVVPVGGK"
BASE COUNT          648 a          322 c          387 g          516 t
ORIGIN      
        1 tattgatgtg aataaaattt ttcctgtaat agtattcaaa gtaatagact gaaaacattc
       61 caataatatt tgaacttata aaaaagaaaa gatgattcaa aaaaagagca ggagacacat
      121 taataatatc atgaataaaa attattacat agaaataaga gagggtcaca atccaggcag
      181 aaaagactgc cgagataaaa cgcagtcttg tgaaagtgta aatccacata atgaccagaa
      241 gcagaccggt gaaatagtaa ttgtgtccca attcactttc ttttgcaatt ccgatcattg
      301 ctgcaattcc aagtccaagt gatacacttg taaaagcgaa cagcaactgg tttattcttc
      361 tgaaaacagg gaaaatggtt gaaataaaag taagtattaa aatcggaacg acaattccaa
      421 atcggatgaa ccatattttt tcttttgatt caggggacat ccatacatcg aggtatgcaa
      481 acagtgcata gagaataatt gcaagaagga tcgcaaatcg gtgaatgctt aaggaagtcc
      541 agtaatagtt ttccttaaaa ctttcttcaa acgatttatt attaaatttt agattaaatc
      601 gatttattaa aattgggtct gtgatatccg tttcattcat tctctagtta caaacttgag
      661 caaagcttat ttagcgatag caaaatttta atgtctgcgg gaaagacttc gatggggagg
      721 cacagcaata gtgcttgaca gaagatctca gttgagaccg atgaagatgc tgaacagaag
      781 gagacagaaa agcagtacaa agtcagccca taaagacctg ctttaaaagc ttccttaaaa
      841 gaaggttgct taaattgaaa ggacactgaa taatcgtcct ggaaacattt tttgctgtgc
      901 agcggctgca tataaacgtt gcgcttaccg ttcgaatcag ctgtgtaatt acagtattaa
      961 ggagattttt atgaaatcta ttttgaaata ttcttttatt gccggggcaa ttttttcaat
     1021 cattggatgc actcgtatag aaacaggtga agtcggactt cgaatagggt tcgacaagca
     1081 ggtgaaaaat gaagaacttc ttcccggttc tttcaatcag accattatcg gttctgtaat
     1141 gacttttcct gtaaaggaag ttgctgtcaa gattgacgat ctgactccgc ttgcaaaaga
     1201 caacagcaca atgaaagact ttgatgctac gataatttat aatatcaatc agactcaggt
     1261 tgccgagatt tacaacagca agaataagtc ttttcatgag acacatgacg gtgatacata
     1321 tctcatgtac aattatattt tcaactctgc aagaaatgca atatacaagt ctgcccgaaa
     1381 atacgaggca ctggatatgg cagacaatcg tgctgttatg gaacaggaaa tcaaggaaac
     1441 aatcatcaaa actttggccg atgaaaaact ggacggcact atcacaattt cgcaggttct
     1501 gatccgaaac attactccgg ctgacagtat tgttgcttcc gcaaatgaac ttgtgaaagc
     1561 aaagaatgaa ttaaagacag aggaaatcaa ggtagccact gcaaaaaaga gaaacgagtc
     1621 tatgcaggca aatcctacta tgattccttt gatgaaagca gaagcggaag cacagtatct
     1681 gcgcgagctt cctgcagcaa ttgccaactt taagggacag actttgatta tcggcggagg
     1741 ggcttctcct gtggttcctg tcggcggtaa gtagaatttc tggatgaaat gatgcggtcg
     1801 taattcctgc gaatcctgta aaaattaatg caggaggtgc gggtttttgt atccctgttt
     1861 aaaggtgaaa act
//
LOCUS       DAOQGJ010000807         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43515_length_1872_cov_3.433682, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000807 DAOQGJ010000000
VERSION     DAOQGJ010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43515_length_1872_cov_3.433682"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PK683_12440"
     CDS             complement(<1..265)
                     /locus_tag="PK683_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH09302.1"
                     /translation="MEINRRENNNIVILDINGEIDLYNAPEIKDVIAKLIEEKKYQII
                     INLEKVSYIDSSGIGALISSLSNLKKYQGGLKIINVAGSVRKVF"
     gene            complement(354..1466)
                     /gene="tgt"
                     /locus_tag="PK683_12445"
     CDS             complement(354..1466)
                     /gene="tgt"
                     /locus_tag="PK683_12445"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNH09303.1"
                     /translation="MNKIFQETSKDSESLARTGILSLNGVTVPTPVFMPVGTRGSIKA
                     LGSEDIDELELDLILANTYHIYLRPGTEILQKMGGLKKFMSYPRAMLTDSGGFQVFSL
                     GDLFKFHSDGVYFKSHIDGSKHEFTPSKVIDIQRIIGSDIMMPLDDCAPYGSDRKRLE
                     LGLERTHRWAESSVEYWEKNPNGQSLFAIAQGGVDKELRIKSLQFMNSLPFPGIAVGG
                     LSVGETRGEFLQMLETLGPHFDGSRPRYLMGVGTIPDILDGVRNGIDMFDCVLPTRNA
                     RNGQVFTSKGKINLRNEIYKDSERPIDEECDCRVCRRYSLGYIRHLHKVKEILGLSLS
                     TFHTLYFMQNFMKKLRMSIEAGEFNIFYEKWKKLYS"
     gene            complement(1463..>1872)
                     /locus_tag="PK683_12450"
     CDS             complement(1463..>1872)
                     /locus_tag="PK683_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNH09304.1"
                     /translation="EHLWLFENARLLREGRLDLADIQHIAETLEDMGKRDFREILSRM
                     RVLMMHLLKWIFQPEQRSSGWKGTIREQRKELNKAFAFSTNLKIYGKEQFLNEYSDAR
                     EIAADETGLPLSSFPGEPPFTFEQVTDENWLPK"
BASE COUNT          485 a          448 c          360 g          579 t
ORIGIN      
        1 cgaaaacctt tctcacagat ccagcgacgt tgataatctt taatccgccc tggtatttct
       61 tcaaattcga aagactggag atcagcgcac caattccaga agaatctata taagatactt
      121 tttccagatt gatgataatc tgatatttct tttcttcaat gagcttggca attacatcct
      181 taatctctgg tgcattgtaa aggtcaattt caccatttat gtctaagatt actatgttgt
      241 tgttctctct tctgttgatt tccattcgaa gtactctact ccttcgtaaa ctctttcttt
      301 aacagtagtt agcattgaat atcactcact gaaaaacgtc aacgaaaatt cgtttaagag
      361 tacaattttt tccatttttc ataaaaaata ttgaactcac ccgcctcgat ggacattctc
      421 agtttcttca tgaaattttg cataaaataa agtgtatgaa aagtggaaag gctcaatcca
      481 aggatttcct ttaccttatg gagatgacgg atataaccta atgaatacct tctgcagacc
      541 ctgcagtcgc attcttcgtc aattggtctt tctgaatcct tgtagatttc attcctgaga
      601 ttgatttttc cttttgaggt aaatacctgc ccgttccgtg cgtttctagt aggtaaaaca
      661 cagtcaaaca tatcaattcc gttcctgacc ccgtcgagta tatccgggat tgttcctact
      721 cccatcagat accttggcct gcttccgtca aaatgcggcc ccaaagtctc cagcatctgt
      781 aggaactccc ctcgggtttc gcccacggag aggccgccga cggcaattcc aggaaacggc
      841 agagagttca taaactgaag agacttaatg cgcaactcct tgtccacccc tccttgggca
      901 attgcaaaaa gactctgccc attcggattc ttctcccagt attcgactga ggattctgcc
      961 cagcggtgag ttctttccag acccaattcc agtcttttcc tgtctgatcc gtaaggtgca
     1021 caatcatcga gaggcatcat aatatccgaa ccaataattc tctgaatgtc aatcaccttg
     1081 gatggggtaa actcatgttt tgagccgtca atatgagact taaaatagac cccgtcagaa
     1141 tggaacttga acagatcacc caggctgaat acctgaaatc ccccgctgtc agtcagcatt
     1201 gctctggggt aggacataaa ttttttcagt ccccccatct tctgcaggat ttccgttccg
     1261 ggtcggagat agatatggta tgtgtttgcg agaatcagat ccagttcgag ctcatcgata
     1321 tcttctgagc cgagggcctt gatgctccct cttgtgccaa ccggcataaa caccggtgtg
     1381 ggaactgtta caccgtttaa ggaaaggatt cctgtgcgag ccagactttc agagtctttg
     1441 gatgtttcct gaaaaatttt gttcacttgg gaagccagtt ttcgtcagtg acctgttcaa
     1501 atgtaaatgg gggctctcca gggaaagagg aaagaggcag tccagtttca tcggcggcga
     1561 tttctcttgc atcggaatat tcattcaaaa actgctcttt tccataaatc ttcaggttag
     1621 tgctgaatgc aaatgccttg ttcagctctt ttctctgttc gcggatagta cccttccatc
     1681 cgcttgatct ctgttccggc tggaagatcc atttcaaaag atgcatcatc aaaacgcgca
     1741 tcctgctcaa aatttctctg aaatctcttt ttcccatatc ttctaatgtt tctgcaatat
     1801 gctgtatatc agcaagatcg agtcttcctt ctctgagaag tcttgcattt tcgaacagcc
     1861 agagatgttc at
//
LOCUS       DAOQGJ010000808         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43589_length_1870_cov_3.189532, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000808 DAOQGJ010000000
VERSION     DAOQGJ010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43589_length_1870_cov_3.189532"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK683_12455"
     CDS             <1..526
                     /locus_tag="PK683_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09305.1"
                     /translation="VTGKSDANGGLVKVTADEGGDISYYKESFDGSGKILGVLKSTNG
                     TTYSTIYGSLNEATNPYSVSFNFNHDTEVTLKLPASQVGTVMDGENFTAYDKFVIVTG
                     GHGHDPNTDHHSIIGMGTYEKKDADTIVDLDELDILYWGRSSEVSTSEVWRKEVDSNH
                     NVKYVKLADKIVLK"
     gene            complement(592..810)
                     /locus_tag="PK683_12460"
     CDS             complement(592..810)
                     /locus_tag="PK683_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNH09306.1"
                     /translation="MGKIAYVNKDDCTSCNQCADNLPKYFQMDDNDTSETHIGGSSIN
                     NAEIPDDDVKKVQKEMDECPGECILWKK"
     gene            complement(878..1762)
                     /locus_tag="PK683_12465"
     CDS             complement(878..1762)
                     /locus_tag="PK683_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNH09307.1"
                     /translation="MNFKNILIYGLGLMGGSLALALKEKDRSVITTGIVRSERSLSEA
                     MELNLADSLYTEEDFLNKDLWNSYDLIVYSLPVDSTCQKINELPASYQGYITDLGSTK
                     KEIIDTVEKKFSKGHRYCSSHPMTGSENSGPRFSKADLYQNKLCILTKPEKSCTESLS
                     LMEKFWTDLGSRTVHIPAKEHDEILAYLSHSPHILSSLLVTWANERKEVGNYTDKSPL
                     ALAGGGFKDMSRIAGSNPEMWEAIIQTNRKSILESLTDFRSRLDSLIQVLSQSDSSGY
                     WKDYFEKAKDHRQKILKL"
BASE COUNT          507 a          371 c          409 g          583 t
ORIGIN      
        1 agtgacgggg aagtcggatg caaacggagg acttgtcaaa gtgactgcag atgagggggg
       61 agacatttct tactataagg agtcgtttga cggatcaggc aaaatactcg gtgtgctgaa
      121 aagcacgaac ggtacaacgt acagcacaat ttacggatca ttaaacgaag cgacaaaccc
      181 ttactccgtt tctttcaatt ttaatcatga tacagaggtg acattaaaac ttcccgcatc
      241 ccaggtcggc acggtaatgg atggggagaa ttttactgct tatgataaat ttgtaattgt
      301 gaccggaggt catggacatg atccgaacac agaccatcat tccatcatag gtatgggaac
      361 ttatgaaaag aaagatgcag atacaattgt tgatctggat gagctggata ttttatactg
      421 ggggcgttct tctgaggtca gtacttctga agtgtggagg aaggaagtgg attcaaatca
      481 caatgtaaag tatgtgaaac tggctgataa aattgttctg aaataggact gtataaaaaa
      541 agcctttgca ctggagtaca aaggcttttt ccggaaagat aataagtcag attatttttt
      601 ccagagaatg cattccccag gacattcgtc catttctttc tggacttttt ttacatcatc
      661 gtccggaatt tcagcgttgt ttatgctgct cccgccaata tgagtttcag aagtatcatt
      721 atcatccatc tgaaaatatt taggaagatt atctgcacac tgattgcaag aggtgcaatc
      781 atctttgttc acatacgcta ttttacccat tcgcgagctc cttgacaggt actttcttgt
      841 aataattcta aaattattta atgaccatga gtgtcaacta gagttttaaa attttctgtc
      901 tatggtcttt tgctttttcg aaatagtctt tccagtagcc tgaactgtcc gactgagaca
      961 atacttgaat cagactgtcc aacctgcttc tgaagtcggt caggctctcc agaatggatt
     1021 ttctgttggt ctgaataatt gcttcccaca tttcggggtt ggagcctgca atcctggaca
     1081 tatccttgaa gcctcctccg gcaagagcaa gaggggattt atcggtatag tttcccacct
     1141 cttttctctc gtttgcccag gttacgagca atgaagacag aatatgaggt gaatgggaaa
     1201 gataagccag tatttcgtca tgctcttttg cgggaatatg cactgtccga gaacctagat
     1261 ctgtccaaaa tttctccatc agactcaatg actcggtaca ggatttttcc ggtttagtga
     1321 gtatgcagag cttgttctga tacagatccg cttttgaaaa tctcggtcct gaattttctg
     1381 aacctgtcat aggatgagag gaacagtatc tgtgaccctt gctgaatttt ttttccactg
     1441 tgtcaataat ttctttctta gtggagccca gatccgtaat gtatccctga taggaagcag
     1501 ggagctcatt aattttttga caggtcgagt ccactggaag agaatatacg atcagatcat
     1561 aggaattcca gagatccttg ttcagaaaat cttcctctgt gtacagactg tctgccaggt
     1621 tcagttccat tgcttctgaa agacttcttt ctgatctgac aatacctgtt gtgatcactg
     1681 atctgtcttt ttccttcagg gcaagcgcaa gagagcctcc catcagtccg agaccgtaaa
     1741 tgagtatgtt tttgaagttc attctggaat ttgttctgcc gcaggataag agcctagtac
     1801 tctgaaataa tttgactgtt ccttgatttc cttcaatata tcgctgataa gcggttcttt
     1861 ttcgtgtccg
//
LOCUS       DAOQGJ010000809         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43683_length_1868_cov_2.936569, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000809 DAOQGJ010000000
VERSION     DAOQGJ010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43683_length_1868_cov_2.936569"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="PK683_12470"
     CDS             complement(<1..1329)
                     /locus_tag="PK683_12470"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNH09308.1"
                     /translation="MKASDYILPTTKENPSDAVVASHILMVRAGLIRKSTSGLYHYLP
                     MGFRILKKIENIVREEMDGSGALEFVLPILTPAELWEKSGRWERMGKEMFRLQDRHNV
                     NYSLGPTHEESFSDLMKPILKSYKDLPKNVYQIHTKFRDEVRPRFGVIRSREFIMKDA
                     YSFHLDQASLDSTYEVMRKTYRMIFQRCGLKTVPVQADSGSMGGSGSEEFMVISPIGE
                     ETLSICGACGYAGNVEKTPVVLEKKAGSDAKLEKVSTPDKKGIEEVAQFMSVHPSETV
                     KAVYLKADNDKNVIVFIRGDRELNEVKLNNLLGTLELRPLGDAECKSLGLVAGYVGPG
                     FSSPEKIKFFFDSTLQPDVPYVTGANEEGFHFKGYILNRDYGKEIKFHDLAKSETGDP
                     CPSCGKPIVQEKGIEVGHIFKLGEKYTKAFDIKVSDKNGKMSLVTMGCYGI"
     gene            complement(1329..>1868)
                     /locus_tag="PK683_12475"
     CDS             complement(1329..>1868)
                     /locus_tag="PK683_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNH09309.1"
                     /translation="EETAFVPLDTVEVGGREYKSSFRLKPIGLLGFRPGVRLDFEEPE
                     NPIGALDAVKLSFYKIYQNVSYSLQGIKMMVTGMISVKKNMSGPIGIAHVAGLSLEQG
                     FMAFMDFFARISIALMIMNLLPIPVADGGHIVLYLYEAVTGKPLPPKAIETIFKLGFI
                     FLLGLGLFVTFYDFMRFVE"
BASE COUNT          503 a          461 c          376 g          528 t
ORIGIN      
        1 gattccgtaa catcccattg tgacaagaga catctttcca tttttatcag aaactttaat
       61 atcaaaagcc ttagtgtatt tctcgcccag cttgaagata tgtcctactt cgatgccttt
      121 ttcctgaacg atgggttttc cgcaggaagg acacggatcc cctgtttctg atttggcaag
      181 atcatgaaat tttatctctt ttccgtaatc cctgttcagg atatatccct tgaagtgaaa
      241 cccttcctca tttgcgcctg tgacgtacgg cacatcgggc tgaagagtgg aatcaaagaa
      301 gaactttatt ttttcaggag aactgaaccc gggaccgaca taaccggcga caagcccgag
      361 agacttgcat tctgcatctc caagcggtct cagttccagt gtgcccagaa gattgttcag
      421 tttgacctca ttcagctccc tgtcacctct gatgaatacg atgacatttt tatcattatc
      481 cgctttcaga tatacagctt tcacggtttc cgaaggatga acggacataa actgcgccac
      541 ttcttcaatt ccctttttat ccggtgtgga aaccttttcg agttttgcat cggatccagc
      601 cttcttttcc agaacaaccg gagtcttttc cacattgccg gcataaccgc aggccccgca
      661 gatggaaagg gtttcttcac cgataggaga aatcaccata aactcttccg agcccgatcc
      721 ccccatgctt cctgaatctg cctgaacagg cacagtcttc agaccgcatc tctggaaaat
      781 catcctgtat gtttttctca ttacttcata agtagaatct aatgatgcct gatccagatg
      841 gaaggaatac gcatccttca tgatgaattc tctcgaacgg atgacgccga atctgggtct
      901 cacctcatct ctgaactttg tatgaatctg gtatacattc ttaggaagat ccttgtagga
      961 tttcagaata ggtttcatca gatccgaaaa agactcttca tgagtaggac cgagactgta
     1021 atttacatta tgcctgtcct gaagacggaa catttccttt cccattcttt cccatcttcc
     1081 gcttttttcc cagagctctg caggagtgag aatcggaaga acaaattcca gcgcgccgga
     1141 accgtccatt tcctcacgga caatattttc tatttttttc agtatacgga agcccatcgg
     1201 cagataatga tacagacctg atgtgctttt acggatgaga ccggctctaa ccataagtat
     1261 atgagatgca acaacggcat cacttggatt ctcttttgtt gtaggtaaaa tataatctga
     1321 agctttcatt attcgacaaa ccgcataaaa tcataaaatg tgacaaatag tccaagaccc
     1381 agaaggaaga tgaaacccag cttgaaaatt gtttctatcg ccttcggggg aagaggcttt
     1441 cctgtgacgg cctcatacag atacaggaca atatgtcctc cgtctgctac gggaatggga
     1501 agaagattca taatcatcag tgcaatggaa attctcgcaa aaaaatccat gaatgccatg
     1561 aacccctgtt cgaggctcag tcctgccaca tgggcaattc cgattggacc ggacatattc
     1621 ttcttgacgg agatcattcc tgtcaccatc atcttgatgc cctgaagaga ataggataca
     1681 ttctgatata ttttatagaa ggacagcttt actgcatcaa gtgctccgat aggattctca
     1741 ggctcttcaa agtccagtct gaccccggga cggaatccca gaagcccgat cggcttcaga
     1801 cggaaagaag atttgtattc tctgcctccg acctccactg tgtccagagg aacaaaggct
     1861 gtctcttc
//
LOCUS       DAOQGJ010000810         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43805_length_1865_cov_3.117127, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000810 DAOQGJ010000000
VERSION     DAOQGJ010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43805_length_1865_cov_3.117127"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..>1865)
                     /locus_tag="PK683_12480"
     CDS             complement(407..>1865)
                     /locus_tag="PK683_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09310.1"
                     /translation="YDDNKVSPAGDRGAFKIINSWGTGWGDRGYGWISYRMFVTLGPN
                     PMVLYDIKTDKPADNQVDNDIKLQNPASVQASNGTYRDRVILSWDAVPGVSAYAVFRK
                     DPSAGDDFESLKVVSKNTGYTDTSVIPDRMYIYKVVSYLSKDVSSDIETAPEASGYAK
                     SEQILLVPEKVTGLLGSLKLTNGTAAVHLQWNSVGNASSYEVVRWDGKKWNAISQARS
                     NSFSDSSPTIGRQNYYCVRARNSQGAGKWSDMFHISAGGDNVPAVPAGLSASMSFPGK
                     IELRWSSVPGASLYVIKRFNYADRTMKEIRTKTAEHTDTDSLIPGAYFSYTVCAGNSA
                     GYSEYTPWVFGKASLNKEPDIQDIADEISSLLSRKVKKIDPNAKTRFTRNLNLSKDDP
                     SEFFAGEWKGKFWDGGKNTQEFSIKVSFEGKKFTAEFRHDSTVKKITGTTVQNANFLE
                     TEGFRMKKVMDKVVKVSIHSHSIYPKDLELSFTKD"
BASE COUNT          496 a          428 c          360 g          581 t
ORIGIN      
        1 tatccaggtg gacggtgaaa atcgctcgtc ggccgtctct tctgaataaa gaaacttgat
       61 tttagcatcg cctgacggcc gcctgagggc tgttataatg atatgcataa atagctgcgt
      121 ttttctttcc ggtaatgcag atttattagt gtagaaaaaa taagtgtaca aaggcttccg
      181 aagtgataca ggagcaggta tgaatgcttc actgaaaata tttttttgta tttcattttc
      241 ggacaggata acgcctgcag tatttcctga atgatgattt ttaaggaatt tcgcatactc
      301 agacaggaag aattgatctt atctgccaga aaattcaaat ctgcacttct actgaaaagg
      361 aaaaatcaga ctctcaaaag gacttgagag tctgaactcc tcctgcctag tctttggtaa
      421 aactcagttc aagatccttg ggatatattg aatgagaatg tatgctgact ttcacgacct
      481 tgtccatcac cttcttcatt ctgaatcctt cagtctcaag aaaatttgca ttctgcacag
      541 tggtgccagt aatctttttg actgtactgt cgtgtctgaa ctcagcggta aattttttac
      601 cctcgaaact cactttaatg ctgaattcct gggtattttt tccgccatcc cagaattttc
      661 ctttccattc tccagcaaaa aattcagaag ggtcgtcctt gctcagattt agatttcggg
      721 taaaccttgt ctttgcattt ggatcaattt tctttacttt tctggagagc agactgctga
      781 tctcatccgc aatgtcctgt atatcaggtt ccttgttcag actggctttt ccgaataccc
      841 atggagtgta ttcactgtat ccggctgaat ttccggcaca cactgtatag ctgaaatatg
      901 ctccaggtat cagagaatcg gtatctgtat gctctgctgt cttagttctg atttccttca
      961 tagttctgtc cgcgtaattg aaccgtttaa tcacatacag tgatgctcct ggaacagaac
     1021 tccacctaag ttcaattttg cccggaaaac tcattgaagc actcagtcct gcaggcacag
     1081 cagggacatt atctcctccg gcagatatat ggaacatatc actccatttt cctgcaccct
     1141 gagagtttct tgccctcaca cagtagtagt tctgcctgcc tattgtagga gaagaatcac
     1201 tgaatgaatt ggatcttgcc tgagaaattg cattccattt tttaccatcc catctcacaa
     1261 cctcataaga agatgcattt cctacagaat tccactgcag atgaacagcg gcagttccgt
     1321 ttgtcagctt aagcgaacct aggagccctg tcactttttc aggcacaaga agaatctgct
     1381 ccgactttgc ataaccggat gcttcagggg cagtttctat atccgaagaa acatccttgc
     1441 tgagatagga tacaacttta taaatataca ttctgtcagg aatcacagaa gtgtcagtat
     1501 atcccgtatt ctttgaaacg actttcaacg attcaaaatc gtcaccggct gaaggatctt
     1561 ttctgaacac cgcataagca gaaacacctg gaacagcatc ccagctcaga atgaccctgt
     1621 ctctgtatgt tccattcgat gcctgaacag aggccggatt ctgaagcttt atatcattgt
     1681 caacttgatt gtctgcaggt ttatctgttt ttatatcata aagaaccata gggtttggtc
     1741 ccagggtgac aaacattctg taggatatcc atccgtagcc tctgtctccc cagcctgttc
     1801 cccaggaatt gatgatttta aatgcacctc tgtcgcctgc cggactgact ttattatcat
     1861 catat
//
LOCUS       DAOQGJ010000811         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43856_length_1864_cov_3.038143, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000811 DAOQGJ010000000
VERSION     DAOQGJ010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43856_length_1864_cov_3.038143"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PK683_12485"
     CDS             complement(<1..1106)
                     /locus_tag="PK683_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09311.1"
                     /translation="MAENNTQEEKHHNKGGRVIGIFTKKVIQNIEFSMKVIWLIFLYI
                     SATWLVLGTLSSQQFQQKIYKMVDEKTKKNYTVAQLISLTEQYEAGLMKLKDIKINVN
                     TQEEKILREDREIDRLRDERKASAAELKDISTRLSTEYKDILQNWVGMKKLMEPAALL
                     AGKDLAKIQASGKLADELKTFQSEMESAKFAEQSSLIESSSARHRDLLAKLEKAAANS
                     ERTDKVIRESEQKLFTSIKEVGKALKEHEKEKDIIQDIEDSALQESLVDLRYLTKMKF
                     HYLATMPAQLLTLILTISMGALGSIIFLTKELFNPMQRSRSIFWYLFRPFLGMVTAIA
                     IFVLIKSGQIVITEVNTEKTAEALNPFFVSFLAI"
     gene            complement(1515..>1864)
                     /locus_tag="PK683_12490"
     CDS             complement(1515..>1864)
                     /locus_tag="PK683_12490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH09312.1"
                     /translation="TYGHDTGDEIIKKVARGILKSCRDLDTVGRYGGEEFSVLLPNTN
                     IETAALVAEKIRTYIEQKAHHSEKYGDLKCTVSIGLSGLNKNFAKVEDLFKDADSRLY
                     NAKANGRNRIEKA"
BASE COUNT          461 a          415 c          359 g          629 t
ORIGIN      
        1 ataattgcca gaaacgacac gaaaaacgga ttcagtgcct cagctgtttt ttcagtattg
       61 acttcagtaa ttacaatctg acctgacttg atcagaacaa aaattgcgat agcagtcacc
      121 attccaagga aagggcggaa aagataccag aaaatggatc tggatctctg cataggattg
      181 aagagttcct ttgtgagaaa tataatgctt cccaaagctc ccatggaaat agtaaggata
      241 agagtcaaaa gctgtgcagg catagtggca agataatgaa atttcatctt tgtgagatat
      301 cgaagatcca caagagattc ctgaagagca ctgtcttcaa tatcctgaat gatgtccttt
      361 tccttttcat gttccttgag tgcctttcct acctctttga tcgaagtaaa aagcttctgc
      421 tccgactctc tgattacttt atcagttctt tctgaattgg ctgcagcctt ttcgagtttg
      481 gcaagaaggt ccctgtgcct tgcagaagat gattcgatca ggctggactg ctcagcaaat
      541 ttcgccgatt ccatttcgga ctgaaaagtc ttcagttcgt ctgcgagctt tccgcttgcc
      601 tgaatttttg caagatcctt gcctgcaagc agagcagcag gctccatcaa ttttttcatt
      661 cccacccagt tttgaagaat gtccttgtat tcagtgctca gtcttgtgga aatatctttg
      721 agttcggcgg cacttgcttt tctctcatct ctcagtctgt caatttccct gtcttctctc
      781 aaaatttttt cttcctgagt gtttacattg atcttgatgt ctttcagttt catcagaccg
      841 gcttcatact gttccgtcag actgatcagc tgcgccacag tataattctt ttttgttttt
      901 tcatccacca tcttgtagat tttctgctga aactgctgcg aactcagagt cccgagcaca
      961 agccaggttg cagagatata cagaaaaatc agccagatga ctttcatgga aaattctata
     1021 ttctgaatga cttttttcgt aaatattcct atgactcttc cgcccttatt atgatgtttt
     1081 tcttcctgtg tgttgttttc agccatcagt ttctccctgt ctgcagttcg acttaaattg
     1141 tacggaaagc cttcttaatg caagaagatt ttttttggaa cccgaattta aaagttgtct
     1201 gttcggataa tatgacggct gattctcctg attctgaaaa gactttttcg atctttactg
     1261 atttcggctg accgataaaa tcctggatac cagtaagctc aaagtacgga ggtgctgatt
     1321 tctcccattc agtaagcttt tttattaact caccttacgc ctgcggatgc aggcggggcg
     1381 atgctgtaca atcagcagga tcggcactga gtaatatgga aagcccgtcg tcattgattt
     1441 gcaggcctct ctgctattat tgatttctaa tagccgtcgc ggcttcaata ctagcgagat
     1501 tagaaatctg agcattatgc tttttctatt ctgttccttc catttgcttt tgcattataa
     1561 agtctgctgt ccgcatcctt gaataaatcc tctactttgg caaaattttt attcaaacct
     1621 gataagccga tgctgactgt gcattttaag tctccgtatt tttcagagtg atgtgctttc
     1681 tgctctatat aggttctgat tttttccgcc accagagcgg ccgtttctat attggtattt
     1741 ggaagcagaa cactgaattc ctctccgcca taccttccga ccgtatctaa atcgcggcag
     1801 gattttaaaa ttcctcgagc aactttttta atgatttcat ctcccgtatc atgcccatag
     1861 gtgt
//
LOCUS       DAOQGJ010000812         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43872_length_1864_cov_2.171918, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000812 DAOQGJ010000000
VERSION     DAOQGJ010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43872_length_1864_cov_2.171918"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..581)
                     /locus_tag="PK683_12495"
     CDS             complement(45..581)
                     /locus_tag="PK683_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09313.1"
                     /translation="MKIISSIFVVVLFSVSISANMRAPQFHYSHASYAVSRSSEGLTV
                     KKEDLSFECPAFQNLTENSDFMNAACDVNAVYELEAEKAGTYRFEFILPSDLETSVSM
                     NGEASMKIKPLIIKKLSGQRVSVTSLNGKGTGLRFTCTMLSQPPEVENTSFPLGWKAV
                     YAVPLMSKVSPSQIGWAI"
     gene            complement(667..>1864)
                     /locus_tag="PK683_12500"
     CDS             complement(667..>1864)
                     /locus_tag="PK683_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH09314.1"
                     /translation="KAAELLCSAKKFCSLSELLCSAKSPGMFLGWGARNCTEITVKIA
                     SLLGMPASVTLQGLSAFPADSPFSVGMGFGNYSVPAAEKAFSGCDCLLAVGTRFSEIP
                     TGSFGMKTPKNLIHIDINPNVFSRNYPASVTLEGRAESILPLLYEELIKIVPSPRNSD
                     ALQELIRREKAAYLKEWTDHKTEGRVNPARFFQELRKQLPRDGYLVADDGNHTFLSAE
                     LFPVYEPCHFISPTDFNCMGYAAPASIGVKIANPEKEVAAVIGDGAFLMTCMEILTAS
                     ALQKGVVYYVFNDGELSQISQGQEIPYNRKTCTVLGNVKFQGIAAAVGAEYLRMETDE
                     QIESVISTARQKAASGVPVIVDVNIDYSKRTRFTSGVVGTNLGRFPLREKIRFILRAL
                     KRKFLG"
BASE COUNT          519 a          444 c          415 g          486 t
ORIGIN      
        1 agcattatcc aggttaaact aagttttaaa gatcaggtgc tcgttcagat cgcccaacct
       61 atttgtgaag gcgatacctt gctcatcaac ggaaccgcat atacggcctt ccaccccagt
      121 ggaaaagaag tgttttctac ctcgggtggt tgcgacagca tcgtacaggt gaacctgagc
      181 cccgtgccct tgccgtttag cgaagtgaca gacacccttt gcccggatag ttttttgata
      241 atcaatggct ttattttcat ggatgcttca ccgttcatgc tcactgaggt ctcaagatcg
      301 gaaggaagaa taaactcaaa cctgtatgta cctgcctttt cagcctccag ttcataaaca
      361 gcatttacat cacaggcagc attcataaaa tctgaattct cagtcagatt ctgaaaagca
      421 gggcactcaa aagaaagatc ttcctttttt acagtgagtc cttcagagga tctggacact
      481 gcataagatg catgactgta gtgaaactgg ggagcacgca tatttgcaga aatagaaacg
      541 gaaaaaagca caacgacaaa aatggatgag atgattttca taattgataa agtgccggaa
      601 tcgacagtga tgtcaagtga aatcagatct gctgaaagat cagttttcag cagatttttg
      661 aaacggctag cctaagaatt tccgtttcag tgcgcggaga ataaagcgaa ttttttcccg
      721 aagcggaaac ctgcctagat ttgttccgac cacccctgaa gtaaaacggg tgcgtttaga
      781 ataatcaata ttcacatcca caatcacggg tactcccgac gctgccttct gtcttgcggt
      841 gctgatcaca gattcaatct gttcatccgt ttccatacgc agatactctg ctcctacggc
      901 tgcagcaatc ccctggaatt tcacatttcc cagaacagtg caggtctttc ggttgtacgg
      961 aatttcctga ccctgagaaa tctgggaaag ttcaccgtca ttgaatacat aatagacaac
     1021 gcctttctga agagctgaag cagtcagtat ctccatgcat gtcatcagaa acgcaccgtc
     1081 accgatgact gcagccacct ctttctccgg attcgcaatt ttcacgccga tggatgccgg
     1141 agcagcataa cccatacagt tgaaatccgt cggagaaata aaatgacagg gttcgtatac
     1201 aggaaaaagt tccgcagaga gaaaggtatg attcccgtcg tcggcaacaa gatatccgtc
     1261 tctcggaagc tgttttctca gctcctgaaa aaagcgtgcc ggattgactc ttccttcggt
     1321 cttatggtca gtccattcct tgagatatgc cgccttttct ctgcggatca gttcctgaag
     1381 tgcatccgaa tttctgggtg atggaacaat ttttataagt tcttcataca gtaaaggaag
     1441 tatagattct gccctgccct ccagagtgac tgatgcggga taatttctgc tgaacacatt
     1501 cggattgatg tctatatgaa tcaggttctt gggagttttc attccgaagc ttcccgtagg
     1561 aatttcactg aaccttgttc cgacagcaag aagacagtcg cagccggaaa atgccttttc
     1621 cgcagccggc acggaataat tcccgaatcc cattccgaca gaaaatggac tgtccgcagg
     1681 aaaagcggaa agtccctgca gtgtcacaga ggcaggcata ccgagaaggg atgcaatttt
     1741 cacagtaatc tcagtgcagt ttcttgcacc ccagccgaga aacattccgg gagactttgc
     1801 actgcagaga agttctgaaa ggctgcagaa cttctttgca ctgcagagaa gttctgcagc
     1861 cttt
//
LOCUS       DAOQGJ010000813         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43910_length_1863_cov_2.866150, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000813 DAOQGJ010000000
VERSION     DAOQGJ010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43910_length_1863_cov_2.866150"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..891)
                     /locus_tag="PK683_12505"
     CDS             complement(358..891)
                     /locus_tag="PK683_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09315.1"
                     /translation="MKYLLCASLLFFLLDCAVPASAERMTVTDFQVNKPVGERIFVVE
                     STGGSMTLPFWISNIPNDNFTDAVRTSLVQSKSFQKISDSMENGWILKIEIIDLDRPW
                     FGTDFKHKLTARYTLTKNGKIIFNEPVTAEGTATMGDALIGVYRAKKANEFAARNSIK
                     AFLNALNDLNAGIQLQQ"
     gene            complement(1250..>1863)
                     /locus_tag="PK683_12510"
     CDS             complement(1250..>1863)
                     /locus_tag="PK683_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002735614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNH09316.1"
                     /translation="WNSYIETVDVLTQDDLKIDVTAVIILRPISSEIYSLQKEIGPEY
                     YKKLVRAEFRTSVRNAMAKYQMIQISKNSPVIANEIKATVNDRIKDKHLEISNVVIDD
                     VVYSKTMLDAIEKKLAKQQELEQMKYELSISEKNIQIAKMKAQADAEASVIRAEGQAK
                     SQTIINSKLTPRYLQYKAFENPNSKFFFIPTDKGNLPVIVNPN"
BASE COUNT          544 a          365 c          334 g          620 t
ORIGIN      
        1 gcttctccgc gttgcctttc tggaggtaag taataatctg cttatcgatt tttgtagtat
       61 atttctgttt ggaatcacag gagtttagaa aaacggctga aacaatcagg attgaaaaat
      121 agagtttttt gagcaggcaa taaaaagctg atccctgaaa agtccggatt ttatcagttt
      181 ttagctgaag agtatttagc agagaatcgt tcataaaatc tgtttgccga tcagattatc
      241 aattgattct attttatcaa tcgaaatttt tcatcagatg atgttaaatt attgtgaagt
      301 gtatggaaaa tataagatcg taaaattctc cgacggacca ctgcacccag tgcctattta
      361 ttgctgaagc tgaattcccg catttaaatc gttaagagca ttgagaaatg cttttatact
      421 gtttcttgcc gcaaattcat ttgctttttt agctctgtaa acaccgatta aagcatctcc
      481 cattgttgcc gtgccctcgg cagtgacagg ttcattaaat attattttcc cgttttttgt
      541 cagagtgtag cgggcagtca gtttatgttt aaaatcagtt ccaaaccagg gtcgatcaag
      601 gtctatgatc tcaattttca gaatccagcc attttccata ctgtcagaaa ttttctgaaa
      661 tgattttgac tggacaagtg atgttctgac ggcatccgtg aaattgtcat tgggaatatt
      721 cgaaatccag aacggaagag tcattgatcc tccggtcgat tcaactacaa aaattctttc
      781 tccgacaggt ttattcacct gaaaatcagt aacagtcatt ctttctgcac tggcaggcac
      841 agcacaatcc agcagaaaga acagaagaga tgcacataat aaatatttca ttttcatcct
      901 atatgcaaac aaaatcaaaa gagctgaaaa aattcaaatc tcaaacaaca aatttgcaca
      961 tatacgcaat ttagtcaaac aaaagcccag ttcattcctt tacatactgc tgtcagtatt
     1021 cattttgcgg tcaaaatcat taattctgcg gaattaatca ggaagaggaa aatcccagct
     1081 cgcaccgtgc tgacaaatat ctgctttcta tgaatcagaa ggttttatgc cgaaagatgt
     1141 gcagacgcga cccgcttttt aaagctattt tcagtaaatc ctttacaaaa cattttccag
     1201 cagtgacgat tcttctcagc cataaaatca gattgtaaag ctgaattttt caattcgggt
     1261 ttacgattac tggaagattt cctttgtctg taggaatgaa gaaaaattta gaatttggat
     1321 tttcaaatgc cttgtactgc aagtaacgag gggtcagctt tgaattaatg attgtctgag
     1381 actttgcctg tccttctgca cggatcactg aagcctctgc atcagcctgt gccttcattt
     1441 ttgcaatctg gatatttttt tctgaaatac tgagttcata tttcatctgc tcgagttcct
     1501 gctgcttcgc cagttttttt tcaatcgcat caagcattgt cttgctgtag acaacatcat
     1561 caatgactac attggaaatc tccagatgtt tatccttgat tctgtcattc acagtagctt
     1621 tgatttcatt tgcaattacc ggactgtttt tggaaatctg aatcatttga tactttgcca
     1681 ttgcatttct gactgaggtt ctgaattcgg ctctcacaag ttttttataa tattccggac
     1741 cgatttcctt ctgcagactg tagatttccg atgaaatagg tctaagaata atcaccgcag
     1801 tcacatctat tttgagatca tcctgagtca gtacatccac tgtttcaatg taggaattcc
     1861 act
//
LOCUS       DAOQGJ010000814         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43914_length_1863_cov_2.488938, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000814 DAOQGJ010000000
VERSION     DAOQGJ010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43914_length_1863_cov_2.488938"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1863)
                     /locus_tag="PK683_12515"
     CDS             complement(<1..>1863)
                     /locus_tag="PK683_12515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH09317.1"
                     /translation="EDTSAINRRLFFWANKIHEFPEKEFSEKLKVFLEKNGKELPEIS
                     SALKEYSEQLKIKDGELKKEVLLFILPSESVHTRNYRFEDETRSRYVSYQTRDASSGK
                     ILEAGFYYNHYRLYIHSITMIFFYIFAGGLVLFIAGIPVFLSGALIRPLDELLEGVRK
                     VEEGNLDVTVPVKVQDEIGFLAGSFNGMVGSIRESKEKLQDYAENLEQKVDERTRELM
                     ATLKQVELLKEQQDGDYFLTTLLLKPLGTVKAENTRLKVDFFVKQKKEFTFRKQKHQI
                     GGDICISQTLNLKGKKYIVFLNADAMGKSMQGAGGILVLGAVFHSIVHRTKSYIAHSE
                     VPPEKWMKNAFKELHKIFESFDGSMLVSLLFGLVEESTGLVYYINAEHPWMVLYRDRI
                     ASFAEHTLSFRKLGHSGLEGEIYVTTFQLHPGDLLIMGSDGKDDVVLSKSKDSRVINE
                     NEELFLKKVEEAEGNLERIYGTLADTYELIDDFSVMSIFFPESDDAIDLELEQKIAEN
                     LELSRKKLQERRSEEALKILRETYELDSGRQETAQLLMRVLMKKKIYREAAKVCKQYL
                     KSNEVNTDMMFRASYCLKMSGSFEEAVDLGERVKLREPRHLRNLVNLADLYAYT"
BASE COUNT          480 a          465 c          340 g          578 t
ORIGIN      
        1 ttagtatatg cataaagatc agcaagattc acaagatttc ggagatgcct cggctccctg
       61 agtttgactc tttcccccag atcaactgct tcttcaaagg aaccgctcat cttgaggcag
      121 taggaagctc tgaacatcat atcagtgttc acttcattgg acttcagata ctgcttgcat
      181 acctttgccg cttccctgta aatcttcttt ttcatcagaa ctctcataag aagctgagct
      241 gtttcctgtc ttccgctgtc aagctcgtat gtttccctga gtatcttaag ggcctcttct
      301 gatcttctct cctgaagttt tttcctcgaa agttccagat tctctgcaat cttctgctca
      361 agctccagat caatcgcatc atccgattca gggaaaaaaa tggacattac tgaaaagtcg
      421 tcaatcagtt catatgtgtc tgcaagagtg ccgtaaattc tttccagatt tccttcggcc
      481 tcttccacct ttttcagaaa gagttcctca ttttcattga tgactctgct gtccttgctc
      541 tttgaaagaa cgacatcatc ctttccgtcg gaacccatga tcagaaggtc tcctggatga
      601 agctggaagg ttgtcacata aatttcccct tcgagtccgg aatgcccaag cttcctgaaa
      661 ctcagagtat gttctgcaaa ggaagcgatc ctgtccctgt aaagaaccat ccagggatgt
      721 tcagcattaa tataatatac aagacctgtg ctttcttcca caagtccgaa cagcagagat
      781 acgagcattg atccgtcaaa actctcgaaa atcttgtgca gttccttgaa ggcattcttc
      841 atccattttt cagggggcac ttccgagtgt gctatatagg atttggttct gtgcacaatg
      901 gaatgaaaga ctgcgccgag aaccagaatt cccccagccc cctgcattga ttttcccatt
      961 gcatctgcat tcagaaatac aatgtatttt tttcctttca aatttaatgt ctgagaaata
     1021 cagatatccc cgccgatctg atgtttctgc tttctgaagg taaattcctt cttctgtttt
     1081 acaaaaaaat ccaccttcag ccttgtgttt tccgccttca ctgttcccag aggcttcagc
     1141 aggagagtcg tcagaaaata gtcgccatcc tgctgttcct tcagcagctc cacctgcttc
     1201 agtgtggcca tcagttccct tgtgcgttca tccactttct gctccagatt ttccgcatag
     1261 tcctgaagtt tttcctttga ctcgcggatg gaacccacca ttccgttgaa agaacctgcc
     1321 agaaagccga tttcatcctg aaccttcaca ggaacagtca catccagatt tccttcttcc
     1381 acctttctta caccttcaag gagttcatcg agaggcctga tcagtgctcc ggacagaaaa
     1441 accggtattc ctgcaatgaa gagaacaagt cctccggcaa agatataaaa gaatatcatt
     1501 gtaatactgt gtatatagag tctgtaatga ttataataga accctgcttc cagtattttc
     1561 cctgaggaag catccctagt ctgatacgaa acatatcttg atcttgtctc atcttcaaat
     1621 ctgtaatttc tcgtatgaac cgactcagac ggaagaataa aaagaagaac ttcctttttc
     1681 aattctccgt cttttatctt gagctgttct gaatattctt tcaaagcgga agaaatttcc
     1741 ggaagctcct ttccgttctt ttcgagaaaa accttcagtt tttcggaaaa ttccttttca
     1801 ggaaactcgt ggattttatt cgcccagaag aaaagccgtc tgttgatggc tgaagtatct
     1861 tca
//
LOCUS       DAOQGJ010000815         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43942_length_1862_cov_3.125069, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000815 DAOQGJ010000000
VERSION     DAOQGJ010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43942_length_1862_cov_3.125069"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1713)
                     /locus_tag="PK683_12520"
     CDS             complement(79..1713)
                     /locus_tag="PK683_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNH09318.1"
                     /translation="MKTEGNEKPAQEESKEYRTSMKGDIPAEYMRSFLATPEQAGAVR
                     KGNLFWYDSLKIGFHSRSRYEGRQNADFNKTTSDYADFIGQTTQVWFLADPSPYFAFK
                     LTLQDARLWGGSQNSGAGGDSRYANTTGAGKVIIPNATSAPTTLRNSTDIREAFIMIK
                     KTEILPVNIQIGRQIFAYGDLKLIGPLNWVYTGFSFDGIRFMYDSKYFSSHLFGTVLS
                     EQHDGPAGLASSNGKSRGSIDDAYFTGSYNTLKPSDLFHIDLYGFGVHKKWIQPASPL
                     TTQDRQRQRDELITGGIRITNRTDKGNLPKGQIFDWTVESAWQKGLTGERVGADWDLL
                     NQTYSGQRVFTEQVKYDARFLSAEAGVHFLEKFRWGVGYTYASGDPNRNDEKVGTWQP
                     LFPQIAGSMPYWNMMNGQSTMVGFQNVKNYSTRLNIKSSFGTFIFALYDIQKAKSQDA
                     WYNVGGAAVSGGSSENRTNNRYQYDANAHLGKRLFYQYDFTYMFTYKEYVSFWAGFSL
                     IQAQDAIRNARQISETATYTFEPASRYYYLMVSALF"
BASE COUNT          499 a          448 c          356 g          559 t
ORIGIN      
        1 catacttccg acaattccgc tgtcagaaat ttgctgaatt cagcagtctt tgcagtgaac
       61 tttcttttta tacttccttc agaaaagagc acttaccatc agataataat acctcgatgc
      121 aggttcaaaa gtgtatgtcg cagtttcaga aatctgtctt gcatttcgga tcgcatcctg
      181 agcctgtatc agcgaaaacc ctgcccagaa agacacatat tctttatatg tgaacatata
      241 agtaaagtca tactgataaa aaagtctttt ccctaaatga gcatttgcat catactgata
      301 tctgttattg gtcctgttct ccgaacttcc tccggaaaca gctgctccgc ccacattgta
      361 ccaggcatcc tgggactttg ccttctgaat atcatataag gcgaaaatga acgttccgaa
      421 agaggatttt atattcagtc ttgtggaata attcttcaca ttctgaaagc caaccatggt
      481 agactgtccg ttcatcatat tccagtacgg catacttcct gcaatctgag gaaacagagg
      541 ctgccaggtc ccgacctttt catcatttct gtttggatcg cccgacgcat aagtatagcc
      601 gacgccccat ctgaattttt caaggaaatg cactcctgct tccgcagaaa gaaatcttgc
      661 atcgtatttt acttgttcag taaacactct ctgtccgctg taagtctgat tcagcagatc
      721 ccagtcggcc cccactcttt cacctgtcag tcctttctgc caggcagatt ctacagtcca
      781 gtcaaatatc tgtcccttgg gaagatttcc tttgtctgtc ctgtttgtga ttctgatacc
      841 tccagtaatc agttcatccc tctgtctctg cctgtcctga gtcgtgagag gagatgcagg
      901 ctgaatccat ttcttatgaa ctccaaaacc gtaaagatct atatgaaaca gatccgacgg
      961 tttgagtgta ttgtaagaac cggtaaaata agcatcatct attgatccac ggctctttcc
     1021 gtttgaagaa gcaagacctg ccggaccgtc atgctgttca gacagaactg ttccgaaaag
     1081 atgagatgaa aaatatttag aatcatacat aaaccgtatt ccgtcaaacg agaatcccgt
     1141 atatacccag ttcagcggac cgatcagttt cagatcaccg taggcaaaaa tctgtcttcc
     1201 aatctggata ttgaccggaa gaatttcagt cttcttaatc atgataaatg cttcccttat
     1261 gtcagtggaa tttctcaggg ttgtcggggc ggatgttgca tttggaataa ttactttccc
     1321 ggcacctgtc gtatttgcat agcgggaatc gccgcctgct cccgaattct gagatccgcc
     1381 ccaaagtctc gcatcctgaa gcgtcagctt gaaggcaaaa taaggtgagg ggtctgccag
     1441 aaaccagacc tgtgtcgtct gaccgataaa atcggcataa tccgatgtag ttttattgaa
     1501 atcggcattc tgtctgcctt catatctgga tctgctgtga aaacctatct tcagactgtc
     1561 ataccagaac agatttccct tcctgactgc ccccgcctgt tcgggggttg caagaaatga
     1621 tctcatgtac tcagccggaa tgtctccctt cattgaagtt ctgtattctt tggactcttc
     1681 ctgtgcaggt ttttcattac cttcagtctt caccggttcc tgcgcagaaa gtcccaagga
     1741 aaaaaacatg tatataaaat ataccaaagt atttagaaaa aaaattttat aattattttt
     1801 cattcaaaga attctgtctc tttccggaac agtcgactaa ttttccgaga attcctgctc
     1861 tt
//
LOCUS       DAOQGJ010000816         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43981_length_1861_cov_2.985050, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000816 DAOQGJ010000000
VERSION     DAOQGJ010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43981_length_1861_cov_2.985050"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1148)
                     /locus_tag="PK683_12525"
     CDS             complement(123..1148)
                     /locus_tag="PK683_12525"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNH09319.1"
                     /translation="MIHYRIGNILESDTDVIINTVNTVGVMGKGLALEFKRAFPENYK
                     QYERAAKEKQLKIGKLFVTQPNHISIKYIVNFPTKSHWKERSKIEYIKDGLEDLVQFL
                     SEHKEIKSIAIPPLGCGNGGLNWNEVKLIIERSLKELAENIDIIIYEPGFNDEKIILQ
                     ADEKLTLARTMFLTALNHYLRLGYSINLLVAQKTAYFLQILGEPLNLVFEKGFYGPYA
                     QKLNHLLKRINGSYVLYDEKQNNPDVEIRLNPAFFDRVKNSQSSLTAEQKKRLEKLSD
                     LIEGYESPYGMELLATVSFILESNPDYSADQVQSVIHNWTKRKKEIMKPSHIVKAYNQ
                     IKKVLAY"
     gene            complement(1150..1794)
                     /locus_tag="PK683_12530"
     CDS             complement(1150..1794)
                     /locus_tag="PK683_12530"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HNH09320.1"
                     /translation="MSFLEDYSDLELYRITHIQNLPYILKLGKITAFTHQNADPDFIG
                     IGDDSLKENRKQRLIEQPPYGSFSDYIAFYFGTRSPMLYNIQNGYLGVKKRGPEEIIY
                     LVSSFGKIKTARLNFVFFDGHANHSLTECFKEEKDFSNLDWESIHAKYWADTEAEPDR
                     KRKKQAEFLVYKEMPIDTITEIGVYSDSSKTAILKFLEKQDLQFKIILHKDWYY"
BASE COUNT          533 a          366 c          312 g          650 t
ORIGIN      
        1 aagtgagata tggaagtgtc agcaaaaaga ctgctatctg atttgaaaac cttctttttg
       61 tattctttga gaatcttctt aatctgttct tcgtgatctt tatagaaagt atatgacata
      121 gattaataag ctaaaacttt ttttatctga ttgtatgctt ttacaatatg gcttggtttc
      181 ataatctctt tctttctttt tgtccaatta tgaattactg actgaacctg gtcagccgaa
      241 taatcaggat tactctcaag gataaaggat accgttgcca gcagttccat tccataagga
      301 ctttcataac cttcaatcag gtcagataat ttctccaatc gttttttttg ttccgctgtc
      361 aatgacgact gagaattttt taccctgtca aaaaaggcag gattcagacg aatctcaaca
      421 tccggattgt tctgcttttc gtcataaagg acatagcttc cgttaatccg ttttaacaga
      481 tgattcaact tttgtgcata aggcccgtaa aaaccttttt caaaaactaa attcagcggt
      541 tctcccagta tctgaagaaa ataggcagtc ttttgtgcaa ccaaaaggtt tatggaatat
      601 cccaatcgca ggtagtgatt aagtgcagtt aagaacattg ttctggcaag agtcagtttt
      661 tcatccgcct gaagtatgat cttctcatca ttaaagcctg gttcataaat aattatatca
      721 atattctctg cgagttcctt cagggatctt tcaattatca gctttacttc gttccaattg
      781 agaccgccat tgccgcagcc gagaggagga atggcaattg attttatttc tttatgttca
      841 gaaagaaact ggactaagtc ttccagtccg tctttaatgt attcaatctt cgacctttct
      901 ttccaatgag attttgtggg aaaatttaca atatacttaa ttgaaatatg gtttggctgt
      961 gttacgaaca gttttcctat tttgagctgc ttttctttgg ccgctcgttc atactgctta
     1021 taattttcag gaaaagctct cttgaactca agcgcaagcc ctttccccat tacaccgaca
     1081 gtatttacag tgtttataat tacatctgta tcagactcta aaatatttcc tatcctgtaa
     1141 tgaatcatct cagtaatacc aatccttgtg cagaataatt ttaaactgca gatcctgctt
     1201 ttctaaaaat ttcaaaatgg cagttttgga actgtcactg taaacaccta tttctgtaat
     1261 tgtgtcaatg ggcatttctt tgtacacaag aaattcagcc tgtttctttc tttttctgtc
     1321 cggttctgcc tctgtgtcag cccaatactt ggcgtggata gattcccaat ccagattgga
     1381 aaagtctttt tcttctttga aacattcagt gagagaatga tttgcatgcc catcgaaaaa
     1441 tacaaagttc agtcttgcag tttttatttt tccaaagcta gaaaccaagt aaataatttc
     1501 ttccggtcct ctttttttta cacccaaata tccattctgg atattataga gcataggtga
     1561 acgggttcca aaataaaagg caatataatc actgaaactt ccgtaaggcg gctgttcaat
     1621 aagcctctgt ttcctattct ctttcaagct gtcatcaccg ataccgataa aatcaggatc
     1681 cgcgttttga tgagtaaaag cagtgatctt tcctaatttt aaaatatatg gaagattctg
     1741 aatgtgagtg atccgatata attccagatc agaataatct tccaagaaac tcattcttcc
     1801 agttcctgaa aaacagagct gggcactttg atgatttcca tattttcggg aatctcattt
     1861 g
//
LOCUS       DAOQGJ010000817         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43984_length_1861_cov_2.760797, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000817 DAOQGJ010000000
VERSION     DAOQGJ010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43984_length_1861_cov_2.760797"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..>1861
                     /locus_tag="PK683_12535"
     CDS             273..>1861
                     /locus_tag="PK683_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH09321.1"
                     /translation="MSKLKLIQLPVPPPAAFASTGNVPLASSSLASAVFLSGAFPGLE
                     TESVGPEITDAMGDRELAEYLAKEEPEFAGFSLYLWNSERSIHIAEELKKKSPGTRIL
                     FGGPEVNEDNLFLLENSSADVLVSGEAEHIILEVLRKLRHFESPPVNGTAFRKHRNEG
                     FEFTQEQNPDFPLNRYPSPYLSGFLNVDPFRSTYLETVRGCKSSCSYCFYPRSSGGEL
                     RTLDIDESINLIRNLKEKGAAELVFLDPTFNHRPGFEKFLDRLSDLNSDGQMKFFAEL
                     RSEGLNRSIAEKLAKAGFYRIELGMQSVNRETLKRVQRYGNPEKVAESAVILKSLGID
                     LLLDLIIGLPGDSPSDVLNGIDFFKKYELEEFVQAFFLSVLPGTKLRTDAVQEGLVYS
                     PYPPYRVIRTESFSEKELLETFFAAEDGLDRRLDEYPRPNLVSCSPDCNDAFSADLDL
                     EIPQDFFRPAARHSTVYFSSASLYEKKDRMISLIRRKIELDPFCTMDFVLCTDVEFPV
                     DLIDVIKGVLNQARQNYLKRVL"
BASE COUNT          490 a          382 c          444 g          545 t
ORIGIN      
        1 ggttctcctt gactgaattg ataatagttt cttccgatgc agtttcctgt ctttccgatg
       61 catcatcctg cttcggctct gatttttttt tctctgttat tttggaagtc ttttgtttca
      121 ttaattctat cctccaatat attagaaaag ggtcaatgaa tataattgac ttttctgctc
      181 tttttgctga acagtttcag ctgatactga tacatcagtt taaagttcct tgacatataa
      241 cctgcctgcg caatataaaa aagttaatcc agatgtccaa attgaaactg attcagctcc
      301 ctgttcctcc tccggcagca tttgcctcta caggaaatgt gcctctggct tcttcttcac
      361 tggcatcagc ggtttttctc tcaggcgcat ttcctggtct agaaactgag tccgtcggac
      421 ccgaaattac ggatgcaatg ggggacaggg aacttgcgga gtatcttgcg aaggaggagc
      481 cggaattcgc aggattttcg ctgtatctct ggaattcaga aagaagcatt catattgcag
      541 aggaactgaa aaaaaagagt cccggaacaa ggatactttt cggagggccg gaagtgaatg
      601 aggacaatct ttttcttctg gaaaattcga gtgccgatgt gctggtgagc ggagaagcgg
      661 aacatatcat tttggaagtt ctgaggaaac ttcgtcactt cgaaagtcct cctgtaaacg
      721 ggacagcatt tcgaaagcac agaaatgaag gtttcgaatt tacacaggag cagaatccgg
      781 attttccatt gaacaggtat ccttcaccgt atctgagcgg gtttctgaac gtggatcctt
      841 tcagatccac ttacctggaa actgtgaggg gatgcaagtc ctcctgctcc tactgttttt
      901 atcccagaag cagcggaggg gaacttagaa ctcttgatat tgatgaaagc ataaatctga
      961 tcagaaatct gaaagagaag ggtgccgctg aacttgtatt tctggatcct acattcaatc
     1021 acaggccggg gtttgagaaa ttcctggaca ggctctctga tctgaacagc gacggacaga
     1081 tgaaattttt tgctgaactc aggtcggaag gactgaaccg gagtatcgct gaaaaactgg
     1141 caaaggcagg attctacagg attgagctgg ggatgcagtc tgtaaacaga gagactctga
     1201 aaagggtgca gagatacggg aatcctgaga aggtggcaga gtctgcagtg atcctgaaaa
     1261 gtcttgggat tgatcttctg ctggatctca tcatcggcct tccgggggac tctccttccg
     1321 atgttctgaa tgggattgat tttttcaaaa agtatgaact cgaggaattt gtgcaggcat
     1381 tttttctgtc tgttcttccc ggaacaaaac tccgcactga tgcagtgcag gaaggacttg
     1441 tatacagtcc ctatcctccg tacagggtga tcagaacaga gagcttcagt gaaaaggaac
     1501 ttttggaaac tttttttgca gcagaggacg gtcttgacag aaggcttgac gagtatcccc
     1561 gtcctaatct tgtgagctgt tctcccgatt gtaatgatgc attttctgca gatctggatc
     1621 ttgaaattcc ccaggatttt ttccgtcccg ctgcaaggca cagtacggtt tatttttctt
     1681 ctgccagtct gtatgaaaaa aaagacagga tgatttctct catcagaagg aaaattgaac
     1741 tcgatccgtt ctgcacaatg gattttgttc tatgtacaga cgtcgagttt cctgttgatc
     1801 tgatcgacgt cataaaaggt gtactgaatc aggctcgtca gaattatctg aaaagagttc
     1861 t
//
LOCUS       DAOQGJ010000818         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_43990_length_1861_cov_2.625138, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000818 DAOQGJ010000000
VERSION     DAOQGJ010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43990_length_1861_cov_2.625138"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1861)
                     /gene="alaS"
                     /locus_tag="PK683_12540"
     CDS             complement(<1..>1861)
                     /gene="alaS"
                     /locus_tag="PK683_12540"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNH09322.1"
                     /translation="AIEYSLEFSLKYLEFDKDRIWITVFENDDEAIEMWVEAGIPRER
                     ITKLGKADNFWGPAGDSGACGPCSELYLDRGVSKGYEGCGTKESCRPGCSCDRFLEYW
                     NLVFNQFNQDTSGVLHPLKQTGIDTGSGLERVALLSQNVDSVYDTDQLQNIIKYIEKH
                     TKDAYSKNPAPFRVLTDHSRSVTFAIGDGILPDKTGRGYVIRRLIRRAVLYAGKIGIR
                     EPFLFRLAEYVSEIYKEDYPELSKNIGYISSVIFNEEKLFLHTLEVGISQMEEILESY
                     RKENKKLFLGRDIFRLYGTYGFPPEMTKEIVQEKGFVFDEKGYEEELEKDRTLSRESW
                     KGKKANALGSLSTEKLEPTVFTGYTELKTEGKVLYILKEEKSVQSVSAGDACALILDR
                     TSFYGESGGQIGDTGFLKKDKFLFKVTDTQKENDVIIHIGTVLEGSAAKGDALTGEVE
                     TERRALLTYHHSGTHLLHAALRKALGTHVSQKASLVSNEYLRFDFSHPKQMTEKEILE
                     VEQAVNSAVSAGAKVKTEVLDIESAKKTGAMAFFEDKYGDRVRVVSMEDFSTEFCGGC
                     HVSNTKEIEYFLIKKEGSPGAGSRRIEAVCGSKVIDYFQEVFQTISSEINEHNL"
BASE COUNT          431 a          459 c          363 g          608 t
ORIGIN      
        1 agattgtgct cattgatctc ggaagaaatt gtctggaata cttcctggaa ataatcaatg
       61 accttggagc cgcagacagc ttcaatcctt ctgcttcccg ctccgggact gccttctttt
      121 ttaattaaaa aatattcaat ttccttcgta ttggaaacat gacatcctcc gcagaattca
      181 gtggagaaat cttccatgct gactactctg accctgtctc cgtacttgtc ctcgaagaat
      241 gccattgcgc ctgtcttttt ggcggattca atgtcgagga cttctgtctt gactttcgcc
      301 cctgcgctga ctgcagaatt cactgcctgt tccacttcca ggatctcttt ttcagtcatc
      361 tgtttcggat gagaaaaatc aaacctcaga tattcattcg atacaagtga tgccttctgg
      421 ctgacatgcg ttcccagagc ctttctgagt gccgcgtgaa gcagatgagt gcctgaatga
      481 tggtaagtga gaagtgctct tctttctgtt tccacctctc ctgtcagtgc atctcccttt
      541 gcagcacttc cttcaagcac tgtccctata tggatgatga catcattttc cttctgagtg
      601 tcagtgactt taaaaaggaa tttatccttc ttcagaaatc cggtatcgcc gatctgtccc
      661 ccggattccc cgtaaaaact tgtcctgtcc agaatcaatg cacatgcatc tcctgcagaa
      721 acagactgaa ctgatttttc ctctttcaga atataaagta ctttcccttc cgtcttaagt
      781 tcagtgtagc ctgtgaatac agtcggttcg agtttttcag tgcttaagct tccgagagca
      841 tttgcttttt tgcctttcca gctttctctc gaaagtgttc tgtctttttc gagttcttcc
      901 tcataccctt tttcatcaaa gacaaatcct ttttcctgaa cgatctcctt tgtcatctca
      961 ggagggaatc cgtatgttcc gtacagacgg aatatgtccc tgccgaggaa aagttttttg
     1021 ttttcctttc tgtaggactc gaggatttcc tccatctggg aaattcccac ttccagagta
     1081 tgcaaaaata atttttcttc attaaaaatg actgaagaaa tataccctat gttttttgag
     1141 agttcaggat agtcttcttt gtagatttca gatacatact ctgccagtct gaaaaggaag
     1201 ggttctctga ttccgatttt tcctgcatac agaactgctc ttctgatgag tcttcgaatt
     1261 acatatcccc ttcctgtctt gtcgggaaga atcccgtcac cgattgcaaa agtcacggat
     1321 ctggaatgat ccgtcagaac ccggaaagga gccggatttt tagaatatgc atcttttgta
     1381 tgcttttcta tatattttat aatgttctgg agctggtccg tatcgtagac tgaatccaca
     1441 ttctgggaga ggagggcgac tctttcgagt cctgaacctg tgtcgatccc tgtctgcttc
     1501 agaggatgca gaacgcctga tgtgtcctga ttgaactggt tgaatacaag attccagtat
     1561 tccaaaaatc tgtcgcagct gcatcctgga cggcaggatt cctttgtgcc gcatccctca
     1621 tatcctttgg atactcctct gtccagatac agttcagagc agggtccgca ggctcccgag
     1681 tctcctgccg gtccccagaa attgtctgcc tttccgagct tagtgatcct ttctctggga
     1741 attcctgctt ccacccacat ttctatagct tcatcgtcat tttcaaaaac tgtaatccag
     1801 atcctgtcct tgtcgaattc cagatatttt aatgaaaact ccagagaata ttcgattgct
     1861 t
//
LOCUS       DAOQGJ010000819         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44044_length_1860_cov_3.065928, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000819 DAOQGJ010000000
VERSION     DAOQGJ010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44044_length_1860_cov_3.065928"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PK683_12545"
     CDS             <1..345
                     /locus_tag="PK683_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09323.1"
                     /translation="VQRQFQVFLDDSVAKYGKDPFDGMEYLKTNLKNLNKVPYISPSG
                     WPVLFAEFERRYKKEGNKMELPIGFFDYIKAAFKNPMAASFYLPILGLIPILMKGYKG
                     NHLDDLKKKHEH"
     gene            complement(884..1270)
                     /locus_tag="PK683_12550"
     CDS             complement(884..1270)
                     /locus_tag="PK683_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09324.1"
                     /translation="MRKILLSSLFIQDLLYNKKKKDILGKAVEKEIKSNSYIYITSLS
                     ALHLLQLYNTRKNEDEVWENLKTLTENILNIGRDELEIYISLRKQFGEQDILMDMSAA
                     VRNGISTVFHSEKLTFPQKMVQISVF"
     gene            complement(1267..>1860)
                     /gene="kdsB"
                     /locus_tag="PK683_12555"
     CDS             complement(1267..>1860)
                     /gene="kdsB"
                     /locus_tag="PK683_12555"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNH09325.1"
                     /translation="DDERIAEAVRSFKGNVVMTSNSHPTGTDRILEVISKTEYKDYDI
                     IVNIQGDEPGIDVGLIDGVTELKLRHRDWEMSTACCRIWDKNELSDPNRVKVAFTKQG
                     KALYFSRSLIPSNFKEEVPVYRHLGIYCYEKEFLLNYSSLPESSLEKSESLEQLRALE
                     NGSSIGVFITDHAGLSVDSPADLEAVAADFRKRGLIA"
BASE COUNT          518 a          395 c          384 g          563 t
ORIGIN      
        1 gtgcagagac agtttcaggt tttcctggat gactctgttg caaaatacgg aaaagatcct
       61 tttgacggga tggaatatct gaagaccaat ctgaaaaatc tgaacaaggt tccttatata
      121 tccccttcag gctggcctgt gctttttgct gaatttgaga gaagatataa aaaggaaggc
      181 aataaaatgg aacttccgat aggattcttc gactatatca aggcagcgtt caaaaatccg
      241 atggcagcat ccttttatct tcccatcctc ggactgatcc ctattctgat gaaagggtac
      301 aaaggaaatc atctggatga tctgaagaaa aaacacgaac actgagaatg atttgactag
      361 tgtaaataaa attatactgt cattattatt ttttctgtct gtttctgcaa cgatgagaag
      421 aacagaaagg ggattgtatt cagaatacag gatactgcat ataatccttc ctccgtccat
      481 tgtacaaatc ccgcctcctg caaggttttg gctttaagtg caaaagactg cggaacttat
      541 tctaaaactt acatagctca ccttacgcaa atacgatatg ctgcctagaa gcggtaattt
      601 ttagaaaaca ttagaaaaac ctaatggatc agtaaaaacg ggagtttgag ggcggtcagc
      661 cctcaagccg ccggcaggcg ttcctaccaa aaacgcccca cctgcgtaag gtgagttaca
      721 tagtatattc tgcggaatca gcgaatgaga tggatctcag ccagtgcatc ttattatgaa
      781 agaatcatcg caaagtgtgc ccgtcctgaa tatactttca gctgttccag tctgacgagt
      841 ccgcctgcag tgagatggat ctcaggaata tgcaggtgat ttctcagaag acacttatct
      901 gaaccatttt ctggggaaat gtcagttttt ccgaatgaaa aactgtgctg attccgtttc
      961 tgactgcggc agacatatcc atcagaatgt cctgctcgcc gaactgcttt cggagtgaga
     1021 tgtaaatctc aagttcatcc ctgcctatat tcagtatgtt ttctgtaagt gtcttcaggt
     1081 tttcccagac ctcatcttca ttttttctgg tattgtaaag ctgaagcaga tgaagtgcag
     1141 ataatgatgt tatatatata taagaattgg atttgatttc cttttcaaca gccttgccga
     1201 gtatatcttt ttttttctta ttgtagagaa gatcctgaat gaacagactg ctgagaagga
     1261 tttttctcaa gcaataagtc ctcttttcct gaaatccgca gcaaccgctt ccagatctgc
     1321 gggagaatcc acactcaggc ctgcatgatc tgtgatgaat acgccgatgg aactcccgtt
     1381 ttccagtgca cggagctgtt caagtgattc cgatttttcg agggaacttt ccggaaggct
     1441 ggagtagttc aatagaaatt ccttttcata acagtagatc cccaaatgtc tgtagacagg
     1501 aacttcttcc ttgaaattgg aaggtatgag tgatctggaa aaatacagtg cctttccctg
     1561 ctttgtgaaa gcgactttta ccctgttcgg atcactcagt tcatttttgt cccaaattct
     1621 gcagcaggct gtgctcattt cccagtctct gtgcctgagt ttcagttcag tcactccgtc
     1681 aatgaggccg acatctattc caggctcatc accctgtatg tttacaataa tatcataatc
     1741 tttatattca gttttactga ttacctcaag gattctgtcc gttccggtag gatgtgaatt
     1801 cgaggtcatt acgacatttc ccttgaatga acggacagct tctgcaattc tttcatcatc
//
LOCUS       DAOQGJ010000820         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44046_length_1860_cov_2.756233, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000820 DAOQGJ010000000
VERSION     DAOQGJ010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44046_length_1860_cov_2.756233"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="PK683_12560"
     CDS             <1..1830
                     /locus_tag="PK683_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09326.1"
                     /translation="AELEEKEFFDILKTADIESKPEFVLMNAVSIGYLKNNKQKISFR
                     FTMSSPAYYIAEYTCRNWEKCKNPESEEHKWLLEKIKKARSDQFSELILFHIAGILGK
                     MESQEEITAFFNILFKKFIEFKAAYPILRIFGKSVSFLPNFNTGTNSLVQKLERQCTE
                     FRQKYGSTKDFKFEREYGTCFSMLEDNLAVIKKRHWVQYMNSFDKSIRDNNFKPLILE
                     ISGKNFNWRDFKNFLSEDSRRNGNFIRNQMASAMRGFTKSFLNVENAVVNVCENLLNA
                     SDSESRLNDFTQLNMEIYEALFKCLEISDEVHDSSGREEKIFRLITKKRINNEKVFEY
                     TANRLYSECESKKTPSYWVQFLLTSLDSDSDSSIHSMCFQYLVETMYRFRSLNMKSQK
                     LILDSVFNDDLTVSEKFLSKERLTLLKILFDAMPDIYAKHKRNLEAAVSEYPVYQKQE
                     VYFVMIKKGDLFLLRYNSKWGDYNWIADRLARGTVKKSKTEINEIIIESLEEKLKVSG
                     KRFKVEILTVATGAYLIKSKRDDKELKEYYPIICTLETADPDFFETLRSDPKHRFFHI
                     SDLTKPINPDVSPAVKMIISKILSKEGILDRIPEVIKSDVFSG"
BASE COUNT          652 a          334 c          369 g          505 t
ORIGIN      
        1 gctgaactgg aagaaaaaga gttttttgat attctaaaaa ctgccgatat agagtccaag
       61 ccggaatttg ttctgatgaa tgctgtatca attggatatc tgaaaaataa taaacagaaa
      121 atatccttcc gtttcactat gtcttcacct gcatattaca tagcagagta tacctgcaga
      181 aattgggaaa aatgcaaaaa tcctgaatca gaagagcaca aatggcttct tgaaaaaatc
      241 aaaaaagcac gatcagatca attcagcgaa cttatccttt tccatattgc aggcatactt
      301 ggtaaaatgg agtcccagga agaaattact gcattcttca atattctttt taagaaattt
      361 attgaattca aggcagctta tccgatcctg cgcatcttcg ggaaatccgt aagttttctg
      421 ccaaatttta atacgggaac aaacagtctt gtccaaaaac tggaaagaca gtgtactgag
      481 ttcagacaga agtatggcag cactaaggac ttcaaattcg agagagagta tggaacatgc
      541 ttttcaatgc tggaggataa ccttgccgta atcaaaaaaa gacactgggt gcaatatatg
      601 aactcatttg acaaaagcat tcgggacaat aatttcaagc cgttgattct tgagatttcc
      661 ggaaaaaatt ttaattggcg ggatttcaaa aactttcttt ccgaagacag caggaggaac
      721 ggaaatttca tcagaaacca gatggcatca gcaatgagag gttttacgaa aagcttcctc
      781 aacgtggaaa atgcagtcgt aaacgtatgc gaaaatcttc taaatgcatc agattctgaa
      841 tccagactca atgattttac tcagctgaat atggaaattt acgaggctct tttcaagtgt
      901 ctggaaattt ctgatgaagt acacgacagc tcaggaagag aggaaaaaat atttcgcctg
      961 attacgaaaa aacgtattaa taatgaaaaa gtattcgaat atactgcgaa cagactttat
     1021 tcagaatgcg agtcaaaaaa gaccccaagc tactgggttc agtttctcct gacaagtctg
     1081 gattcagaca gtgacagcag cattcattcg atgtgctttc agtatcttgt agagactatg
     1141 tacaggttcc gatctttgaa tatgaaatct cagaaactga ttctggattc agtattcaat
     1201 gatgatctta ccgtttctga aaaattcctt tcaaaagaaa ggctgactct cctgaaaatc
     1261 ctttttgacg caatgcctga tatttatgca aaacacaaaa gaaatctgga ggccgcagtt
     1321 tcggaatatc ctgtatacca gaaacaagaa gtatattttg taatgataaa aaagggagat
     1381 ctatttcttc tgagatacaa ttcaaaatgg ggagattata actggatcgc agacagactc
     1441 gctagaggaa cagtcaaaaa atcgaaaaca gaaatcaatg agatcattat tgaatcgctg
     1501 gaagagaaac tgaaggtctc aggcaaacgg ttcaaagtgg agatattgac tgttgcaacc
     1561 ggggcatatt tgataaaatc aaaacgggat gataaggaac tgaaggagta ctatccgatt
     1621 atctgcactc tggagacagc agatcctgat ttttttgaaa ctctccgaag tgaccctaag
     1681 cacagatttt ttcatatcag cgatttgacg aaaccgatta atcccgatgt cagcccagcc
     1741 gtaaagatga tcatttctaa aatactgagc aaagaaggaa ttctcgatag gataccggaa
     1801 gtgataaaat cagacgtttt ttccggttga ctgcataact tgatctaaaa ccctttcaat
//
LOCUS       DAOQGJ010000821         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44295_length_1854_cov_2.659811, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000821 DAOQGJ010000000
VERSION     DAOQGJ010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44295_length_1854_cov_2.659811"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PK683_12565"
     CDS             <1..317
                     /locus_tag="PK683_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09327.1"
                     /translation="FVYPAHINPIFDNRIPASFRRDASDMHNKIFAHEVNGKIKMKYA
                     DVDYINRSIEKLEGERVKRGLASVPDANRLDKPKDEPAPAAPKENTNKSGSDKQPTPP
                     TK"
     gene            360..1127
                     /locus_tag="PK683_12570"
     CDS             360..1127
                     /locus_tag="PK683_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNH09328.1"
                     /translation="MFFLFSKILTILLFPLPLCILSGFFFSIFLIKGTRNKILSITPI
                     LLLTFFSIPYTSQILIRSLEDEFPAVDFQKIEKADAIIVLGGMINPLSIHREKPELLS
                     TADRLTDSIIMFRQKKAPYILFSGGSGILFQGDVSEARTAKKFLTDMGIPEDQILLED
                     KSRNTKENAVFSAGILREKKMKKIILVTSAFHMKRSLLVFEKEGFEIQIFPTDYKSLY
                     WTWNWDTAVPSIGALDTSTISIKEWIGLISYRIAESI"
     gene            1386..>1854
                     /locus_tag="PK683_12575"
     CDS             1386..>1854
                     /locus_tag="PK683_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09329.1"
                     /translation="MKTLFFLFAIICSSNLKSEQEYSKSRYRLLASGGLFINADLLPI
                     STQGDISYKKSYIGVLGLNYDLQKKLGILNLELEGLLGKHKGEMNHSELGGVLVARIP
                     NLILKPMSFAFGEGLSWASENPFLENRKKGWDRGSFNTENIESRPFLNYLMFEL"
BASE COUNT          608 a          359 c          362 g          525 t
ORIGIN      
        1 cttttgttta tcctgctcat atcaatccta tctttgacaa cagaattcca gcttctttca
       61 gaagagatgc atcagatatg cacaataaga tttttgctca tgaagtaaac ggcaagatca
      121 aaatgaaata tgctgatgta gactatatca acagaagcat tgaaaaactg gaaggtgaga
      181 gagtaaaaag aggactggca tctgttccag atgcaaacag acttgacaaa ccaaaagacg
      241 agcctgcacc ggcagctcca aaagaaaata caaataagtc aggctcagac aaacagccta
      301 cacctcctac aaaataatca tttaacatac tgcgaagcca gacttcgcag tttttccctt
      361 tgtttttttt attctccaaa atactgacca tacttctttt tcctctgcct ttatgcattc
      421 tgagcggctt tttcttttca attttcctga tcaaaggaac aagaaataaa attctttcca
      481 ttacccctat tcttctgctg accttttttt ctattcctta caccagtcag attctgattc
      541 gaagtctgga agatgaattt cctgcagtgg attttcaaaa aatagagaag gcagatgcaa
      601 ttattgtact gggtggaatg atcaatccgc tttccattca cagggaaaaa ccggaacttc
      661 tgagcactgc cgacagactg acagattcca tcattatgtt cagacagaaa aaagccccat
      721 atatcctgtt ttcgggagga tcagggattc ttttccaagg agatgtatct gaagcaagaa
      781 cagctaaaaa gtttctcaca gatatgggaa ttcctgagga tcagatattg ctcgaagaca
      841 agtccagaaa cacaaaagaa aatgccgtct tcagtgctgg aatactgaga gaaaagaaaa
      901 tgaaaaagat cattctagtc acttccgcat ttcatatgaa gagatcactg cttgtctttg
      961 aaaaggaagg atttgaaatt cagatttttc cgacagatta taaatcactg tactggacct
     1021 ggaactggga cactgccgtt ccttccatag gagcactcga cacaagtaca atctcaatca
     1081 aagaatggat cggattaatc agttatcgaa tcgccgaatc gatctagtac tattgacagt
     1141 aaaagaacaa acagaaatta cagagagatt gcaattagat cgatgaatcg gcatcaatct
     1201 cactctgaaa agcgaccaat aggatgaatg actgctgctt tttttacggg tcgtttttgg
     1261 attggagatt ttgagaattc tgaagccctc agtttttgaa aaggaatgtg ctatccgttt
     1321 tttcttggat caatgtaaaa aaagatctta tgaagtattc tgcctgtcta attctattga
     1381 acactatgaa aacactattc ttcctttttg ccatcatttg ctcatcaaac ctgaaatctg
     1441 aacaggaata ctctaaaagc agatacaggc tccttgcctc aggaggactg tttatcaatg
     1501 cagacctgct tccgatttcc actcaaggtg atatcagtta taagaaatca tacataggtg
     1561 ttttaggact gaattatgat ctgcagaaga agctgggaat actgaatctg gaactggagg
     1621 ggctcttagg aaagcacaaa ggggaaatga atcattcgga acttggcggt gtattggtag
     1681 ccagaattcc aaatctgatc ttaaaaccaa tgagttttgc tttcggggag ggactttcct
     1741 gggcaagtga aaatcctttt ttggaaaaca gaaaaaaagg atgggacaga gggagcttca
     1801 atacagaaaa cattgaatcc agaccatttt taaactatct gatgtttgaa ctgg
//
LOCUS       DAOQGJ010000822         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44360_length_1852_cov_3.577073, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000822 DAOQGJ010000000
VERSION     DAOQGJ010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44360_length_1852_cov_3.577073"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PK683_12580"
     CDS             <1..1201
                     /locus_tag="PK683_12580"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09330.1"
                     /translation="FIFIAVTVLSFSAESFLRKYPNVKYDLFTAYLILSFFLAYEIAA
                     RIGVGKIIREGRKPLELIRYGNAFIETSLPSAAILNFIRAGSDPLDALLSPVSMSYFF
                     FIILSTMRLSFGLCIFTALTAVIEYTAIILYFSSMPGWNAKGMISGEVPSLLLRVMFF
                     LVSGLLAGLVSIQIRKSFLNSLNGIAERKQLMDIFGQHVSPAVVDRLLSQKLEMESEM
                     RHVCILFLDIRNFTKFSEERGPAEVVNYLNALFGFMIEIINRNHGIINKFLGDGFLAV
                     FGAPISDGKDCFNAVQASREILASVHSYCAEGKIVQTRIGIGIHSGEAVTGNVGSEIR
                     KEYTIIGDTVNLASRIEQLNKQYGTEILISESVLEKLTDERDSFKKVDTVSVKGREEA
                     VTVYTLI"
     gene            complement(1203..>1852)
                     /locus_tag="PK683_12585"
     CDS             complement(1203..>1852)
                     /locus_tag="PK683_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNH09331.1"
                     /translation="QVEQLLDGILTSEQTESERIDMNMTLSPEEKAQLKKEAGKKTLR
                     DYAAVNLIIRTGLRTVEVSRANIGDIRKKGKDILLYVQGKGRKEKKEFVILKPGAFEP
                     IRMYLNLRKFTKPELPLFTSVSNRNAEERLSQDYISRIFKKALRNIGIDDSRITAHSG
                     RHTAAHLYLEAGGSIVKLQEMLRHASVDTTRFYTAGMERLISPAEEVLEDYLEDI"
BASE COUNT          457 a          401 c          403 g          591 t
ORIGIN      
        1 atttattttt attgcagtga ctgtactcag cttttccgca gaaagctttc tgagaaagta
       61 tccgaatgta aaatatgatc tttttactgc atatctcatt ctaagttttt tcctggctta
      121 tgaaatcgct gcaaggatcg gtgtaggaaa aatcatacgg gaaggaagaa aaccgctcga
      181 actgataagg tatggaaatg cgttcatcga gaccagtctg ccttctgctg ccattctcaa
      241 ttttatccgt gcaggaagcg atcctctgga tgcacttctt tctcctgtct ccatgtccta
      301 tttctttttc attattcttt ccactatgcg gctcagcttc ggactctgca tttttacggc
      361 ccttactgcc gtcattgaat atactgcaat tatattatat ttttcttcca tgcctggatg
      421 gaatgcaaaa ggaatgattt ccggagaagt gccgagtctt cttctcaggg taatgttctt
      481 ccttgtttcc ggacttctgg caggcctagt ttccattcag atcagaaagt ccttcctgaa
      541 ttcgctgaac ggaatcgcag aaagaaagca gctgatggat attttcggcc agcatgtctc
      601 ccctgcagtt gtagacaggc tgctgtcgca gaaactggag atggaatccg agatgaggca
      661 tgtctgcata ctgtttctgg atataagaaa ttttactaaa ttttcggagg agaggggtcc
      721 tgcggaggtc gtgaactatc tgaatgccct ttttggtttt atgatagaga tcatcaacag
      781 gaatcacggc atcatcaaca aatttctcgg agacggtttc cttgcggtgt tcggtgcacc
      841 tatctctgac ggaaaagact gtttcaatgc agtccaggca tcccgggaaa tactggcttc
      901 ggttcattca tattgtgcgg aaggaaagat tgtgcagaca aggatcggaa taggaattca
      961 ttcgggagag gccgtgacgg gaaatgtagg ctctgagatc agaaaggaat acacaattat
     1021 aggagacact gtcaatcttg catcgcggat agaacagctg aacaagcagt atggaactga
     1081 aattctgatt tcagaatctg ttctggaaaa gctgactgat gaaagggatt cattcaaaaa
     1141 agtggataca gtttccgtaa agggcaggga agaggctgta actgtctata ctttgatctg
     1201 agtcatatgt cttcgagata gtcttcgaga acttcttctg caggactgat cagtctttcc
     1261 attcccgccg tataaaacct cgtagtgtcc actgaagcat gtctcagcat ttcctgaagc
     1321 tttacaatgc ttcctcctgc ctcaagatac agatgagcag ctgtatgcct tcctgaatga
     1381 gccgtgatcc tggaatcgtc aataccgata tttcttaatg cttttttaaa tattctggat
     1441 atgtaatcct gggaaagtct ttcctctgca tttcggttgc tcactgaagt gaaaagcggc
     1501 agctccggtt tcgtgaattt tctcaggttc aaatacattc tgatcggttc aaatgctccg
     1561 ggcttcagaa ttacgaattc ctttttctcc tttcttcctt ttccctgcac atacagaaga
     1621 atatcctttc ctttttttct gatatcacct atgttcgcac gggatacttc cactgttctg
     1681 agtcctgtac gtatgatcag gttgactgcg gcatagtctc tcagagtttt ttttcctgct
     1741 tccttcttca actgtgcttt ctcttccgga ctcagtgtca tgttcatatc aattctttct
     1801 gactctgtct gttctgaggt aagtattccg tcaagaagct gctcgacctg tt
//
LOCUS       DAOQGJ010000823         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44368_length_1852_cov_3.278798, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000823 DAOQGJ010000000
VERSION     DAOQGJ010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44368_length_1852_cov_3.278798"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PK683_12590"
     CDS             <1..739
                     /locus_tag="PK683_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09332.1"
                     /translation="LAAGRIFSSAGSWKKGKFALILFLILWGAPLLKFRILQVELFRS
                     PASDSQSAVLEISESSVCRNRQNYFFYPEYINQFSLMETFLAGCMTVPVTEFNIPSFR
                     GFFHEKEIFKYKLLIKNPYENQFTEAGSIAEILASDERIFPIGPDIDWGNSVWPAVIY
                     LKKKSWIEAAVYVKTDMIFSFTADEKEIPSVLFSYGAGSDVGTHLSPESRRCVSRECE
                     YAYKISKGYHFLRIENIIKMNIKRIED"
     gene            1096..>1852
                     /locus_tag="PK683_12595"
     CDS             1096..>1852
                     /locus_tag="PK683_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020783212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNH09333.1"
                     /translation="MKLIKKKNITGIQEPLLLVILDGVGATDRGAAEGNAIAAANIPV
                     LKGLWSGSPTIRIQAHGKAVGMPSDDDMGNSEVGHNVLGCGRVFDQGAKLVSNSINSG
                     ELFAGAAWNWILEPLKGSSDRTLHLIGLLSDGNVHSHIDHLKALIAGAKKAGVKRVRV
                     HALLDGRDVPEKSALEYTEPFEAFLKELRSSEFDVKIASGGGRMTVTMDRYDADWSMV
                     ERGWKFHVLGEGRAFSSIKEAIQTFREEDPKVID"
BASE COUNT          564 a          342 c          421 g          525 t
ORIGIN      
        1 ccttgctgcg ggaagaattt tctcttcagc aggaagctgg aagaagggaa aatttgccct
       61 cattctcttt ttgattctat ggggagctcc tttgttaaaa ttcagaatac tgcaggttga
      121 acttttccgt tctcctgcat ctgattcaca gtccgctgtt cttgaaatct ccgaatcctc
      181 ggtttgcaga aacagacaga attatttctt ttacccggag tacataaatc aattttcttt
      241 aatggaaacg tttcttgcag gatgtatgac agttccagtg actgaattta atattccttc
      301 cttccgggga tttttccatg agaaggaaat ttttaagtat aaattattga taaagaatcc
      361 ttatgaaaat cagtttacgg aagcaggatc tattgccgaa attctggcgt cagatgaaag
      421 aatttttcca attggtccgg atattgactg gggaaattct gtatggcctg cagtgatata
      481 tctgaaaaaa aagtcatgga ttgaagcagc agtgtatgtg aagacagata tgattttttc
      541 ttttacggca gacgagaaag agattccttc agttctattt tcttacgggg caggcagtga
      601 tgtcggaact catttgtctc cggaatcccg cagatgcgtc tcacgggaat gtgagtacgc
      661 ttataagatt tccaagggtt atcattttct cagaatcgaa aatatcatta aaatgaatat
      721 aaaaaggata gaagactgat gcagaaagtc tccataaaaa ccgcattttc cgaaaatgta
      781 aaatagagtt gttgaaaaat tagtttcacc aactctattt ttaatttttc taaaaaacct
      841 gcagcacgct gaagcgcaca aacttgagag ccgctgctga gttaatcctg ccggaggttt
      901 cgctagctgg ctaaaatccc tatttagctt ctgatgaaag ataatttctt gattttttca
      961 acaaatctaa taggaacaga atttcttcca atttaagaaa gacgatttat gacagaaaat
     1021 gacagtcctt tttctattcg acaattgaat gcagaaaatt caaatcgttt tataacgcaa
     1081 taatatgagg gaattatgaa actgattaag aaaaaaaata ttacaggaat acaggaaccg
     1141 cttctgctcg tcattttgga cggggtggga gctacagaca gaggagctgc tgaaggcaat
     1201 gcaattgcag cggcaaatat ccctgttctg aaaggacttt ggtcaggttc gcctacaatc
     1261 cgaattcagg cacatggaaa agcagtgggg atgccttctg acgatgatat gggcaactct
     1321 gaggtcggtc ataatgttct gggatgcgga agagtgtttg atcagggtgc aaaactggta
     1381 tcgaattcca tcaactccgg agaactgttt gccggtgctg cctggaactg gattctcgaa
     1441 cctctgaaag gaagttcgga cagaactctt catctgatcg gtcttctctc cgacggaaat
     1501 gtgcacagtc atatagatca tcttaaggca ctgatcgcag gtgcaaaaaa agcaggagtc
     1561 aaaagggtca gagttcacgc acttctcgac gggcgcgacg ttcctgaaaa atccgcactg
     1621 gaatacactg aaccttttga agcctttctg aaagaactga gaagcagtga atttgatgtg
     1681 aagattgctt ccggcggcgg aagaatgaca gtcactatgg acagatacga tgctgactgg
     1741 tcgatggtgg aaagaggctg gaaatttcat gtgctgggag aaggacgtgc attttcttcc
     1801 attaaagaag caatacagac atttcgagaa gaagatccga aagtcataga cc
//
LOCUS       DAOQGJ010000824         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44410_length_1851_cov_3.200445, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000824 DAOQGJ010000000
VERSION     DAOQGJ010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44410_length_1851_cov_3.200445"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK683_12600"
     CDS             complement(<1..414)
                     /locus_tag="PK683_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH09334.1"
                     /translation="MAGPIIRTYKAGSIVYFEKDKADDIFVLQNGRIILTYLSADGKT
                     EIKEDVKLGEFFGVKSSLGRYPREETAQVLGGATVLVFKMADFELFVAQKTHLILKMM
                     KVFSSQLRQIHHKVREQLGQFGDMKSAAFELMNVAE"
     gene            complement(470..1018)
                     /locus_tag="PK683_12605"
     CDS             complement(470..1018)
                     /locus_tag="PK683_12605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH09335.1"
                     /translation="MIEFLPKASSEKEKPEKKEEKVIIRHESKKEIPVPELPKKKNTG
                     GVTFTHPIQNGNTPLTGFQNPVQNRMDVAKTYSDSLSEPFHGIAVKMDSSSDTAISAK
                     NGKVMMVGYMDGYDNFVIVQHKEDVYSVYAHLDKIHVAEGQTISIGSGVGRVIPQKNL
                     YFQISRGHKTFDPMQILKKKQD"
BASE COUNT          543 a          395 c          316 g          597 t
ORIGIN      
        1 ctcagcaaca ttcatcagtt caaatgccgc cgatttcata tcgccaaact ggccgagctg
       61 ctcccttact ttatggtgaa tctgtctgag ctgactggaa aaaaccttca tcatttttaa
      121 aatcaaatga gttttctgtg cgacaaacag ttcaaaatca gccatcttga atactaaaac
      181 agttgcaccg cctaaaactt gagcagtttc ttccctggga tatcgtccta atgacgattt
      241 taccccaaaa aattcaccga gcttgacatc ttccttaatc tcagttttcc cgtcagcaga
      301 cagatacgtc agaattattc ttccgttttg cagaacaaat atatcatctg ccttgtcttt
      361 ctcaaagtaa actatggagc ctgctttata tgttcttatg attgggccag ccaaaataga
      421 tccctctatt catacatatt aaagatcgaa aaaaaatcca gttttttttt caatcctgtt
      481 ttttctttaa gatctgcata ggatcgaacg ttttatgccc tctggatatc tgaaaataga
      541 gatttttttg cggaatgacc cttcccactc ctgaaccgat ggaaattgtc tgcccttcgg
      601 ccacatgaat cttgtccaaa tgagcataaa cggaatacac atcttcttta tgctgaacaa
      661 tcacgaaatt atcgtaaccg tccatataac cgaccatcat tacctttccg ttttttgcgg
      721 aaatcgcagt atcggaactg gaatccattt tgacagcaat tccatgaaat ggctcactta
      781 aactgtctga atacgttttg gctacatcca ttctgttctg cactggattt tgaaatccgg
      841 taagcggggt attgccgttc tgaatcgggt gagtaaaagt cacccctcct gtatttttct
      901 tctttggaag ttcaggcacc ggaatttctt ttttcgattc atgacggatg atgacttttt
      961 cttccttttt ttcaggcttc tccttttctg aagaggcctt gggaagaaat tctatcattt
     1021 cagccgacgt gcttacaaaa gggaaaatga cgaataaaat caataattta ttttgcatcc
     1081 ggatgacctt cgctttgaaa attacagaca gaatgtccgt aaaagtacag ctctccgata
     1141 tataaaaaat catgcaagaa gatttccaat tacgagagaa aatttgaaat tattacatcc
     1201 ttcaaaattt ttttccggtt tttttaggtg ttcttcctca aaaaaactgt aacctcgcgg
     1261 agcatctgtt tctaaatccg gcacgggctt aaaacgatcc ggtcttcgca ggcctgaagc
     1321 ccctgttttt actgatccat tagatttctc taatgcttcc tggaaattgc atattccagg
     1381 cataatatct tttttttggg taaagcaggg agatagacag aagctggact tttccgcaac
     1441 gaaaataaag catccgttca aaatactgaa caggtactgc agtaaaatct gaattcagga
     1501 atattagata attctaatac tattcatttt gacaggaaac actgtccttc gaattctttt
     1561 attccctgcc agtaatatat gccgacagct tgagggttcg cagtaccctt ttttgcatta
     1621 gttcatagaa tctgctgaat ttcgtatcat ataatgaagc ctggacggca gctattttgc
     1681 gaaatacgaa tcaataaaat tcctcgagca ggaattcaat tgccttcaga aatcaggaac
     1741 tttcaaaggt caatttctca atcactgaat ctggaatcaa atctcgaaca tagttcggaa
     1801 taaaagtctt ctgtttatca gagtaaaaaa atcttgcttg tttttagaat c
//
LOCUS       DAOQGJ010000825         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44468_length_1850_cov_2.704735, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000825 DAOQGJ010000000
VERSION     DAOQGJ010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44468_length_1850_cov_2.704735"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..807
                     /locus_tag="PK683_12610"
     CDS             262..807
                     /locus_tag="PK683_12610"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HNH09336.1"
                     /translation="MNSFEEIYIKETIKVFLSQKELAEKALVQINLNSEFFQNLGERS
                     HSLAVNVKHIAGNLESRWTDFLDSDGEKPNRNRDNEFIIFPEDSREELMKKWENSWNI
                     LINSLKLLNADNLGNTVFIRREPHSIPLAIQRSLAHTSYHVGQILYISRLVKSGDWKW
                     LTIAPGNSSNFNSEMNDRFKQ"
     gene            1541..>1850
                     /locus_tag="PK683_12615"
     CDS             1541..>1850
                     /locus_tag="PK683_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH09337.1"
                     /translation="MANFSISSQLIDATSKIVNILRQIKEINSTTKLIAINAAIESSR
                     SAQLSDNFSALSEQVRTLSTRSEESFTEIQSLIDSLRIYCAKAIAVRLADLSIDLIDK
                     I"
BASE COUNT          579 a          360 c          419 g          492 t
ORIGIN      
        1 gagaagaaaa ctggtttctt gctccgagtc ggcttagtta tggaagtgaa aaatttcagt
       61 gtattttcgg cctgatgaaa atttattctt ttcggcagtt acagtaagtg ctaaattagg
      121 attcgactcg gggaaattgc acttgagtct gaggaaggtg tggcgcactt tcttgcctca
      181 ctgcggcgtc ggctgtgatg gacggtatac ggttttattg aatttcaaac cgcaccaata
      241 tttactgtaa agcaggaaac catgaattct ttcgaagaaa tatatataaa agaaacaata
      301 aaagtgtttc tatcgcagaa ggaacttgcc gagaaggcat tagtccagat caacctgaat
      361 tctgaatttt tccaaaacct tggggaacgg tcccacagcc tggctgttaa tgtgaagcat
      421 attgccggaa atctggaatc ccgatggaca gactttttag attcagatgg cgaaaaaccg
      481 aatcgaaacc gtgataatga atttatcata tttccggagg attccagaga agaattaatg
      541 aagaaatggg agaattcatg gaatattctg atcaattctt tgaaactgct gaatgcagat
      601 aatcttggaa acactgtatt tatccggagg gaacctcatt cgattccttt ggccattcaa
      661 agatctcttg cgcacacttc ttatcatgtg ggacaaattc tatatatttc aaggctggta
      721 aaatccggag attggaaatg gcttactatt gcccccggaa atagcagtaa ttttaattcc
      781 gaaatgaatg ataggtttaa acagtaaatt taaaaaaaac agcgtcggat aacagcaggg
      841 tgagatcaaa tcaggcggtg ttgaaaaatg tatctcggaa cagcctgaag aaatacggaa
      901 aaaattaaaa gcggattcat tctgccattc agaaatcagc acccggttct gcagaaaccc
      961 tcagttactt tcagatgcca ggatactttt acgaaggata tgcatataat gaaattagtg
     1021 aaagtaagta taaaagtcat gaagggaagc aagaaatagg aaagaattgt ccagatttcc
     1081 aatgaaataa agagctgtac atgagacact gattaaaacg ccaatcctat gagcacagag
     1141 ggctgattga atgtgattca tcgcgaagaa gtccgcattt atggaaactg atatgtgtac
     1201 tgaagatatt cttaaaagaa atccggatgt gaaaaatccc cggcactcgg acattgaagc
     1261 tcccacttca gcccctgcgc gacatcagga gtggccgtcc ttgctagcag gctctgcatc
     1321 cggcgaccga agggaggcct gcagggattc accgctaggt gaatgggggg tcagcaggct
     1381 tcgccttctt cgctgtagct ctcgaaggct caccggcatg attcttgctt gtagaaacct
     1441 ctgattcagc acagtaaaaa aaatggcgag atggacgcta tttgcttaaa acctgagcat
     1501 actgtccaaa ctaaagctgt gctttataac ggagaggtct atggcaaatt tttctatcag
     1561 ctctcagctc attgatgcca catcaaaaat agtaaatatc ctgcgtcaga tcaaggaaat
     1621 caacagtacc actaaactga tcgcaatcaa tgctgcaatt gagtcttcca gatcggctca
     1681 gctttccgac aatttttctg ctctttcgga acaggtgaga acattgtcta caaggtctga
     1741 agaatcgttt acggaaattc agagtctgat tgacagtctg cggatttact gcgcgaaggc
     1801 aattgccgtg agacttgccg atctttccat cgatctgatt gacaagatag
//
LOCUS       DAOQGJ010000826         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44490_length_1849_cov_3.933110, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000826 DAOQGJ010000000
VERSION     DAOQGJ010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44490_length_1849_cov_3.933110"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..889
                     /locus_tag="PK683_12620"
     CDS             365..889
                     /locus_tag="PK683_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09338.1"
                     /translation="MWSGAVTMYGIGMKEKDVVSFIDYLLKNSEKNEKKAFLEYIKSE
                     YSAKDFEDLQKKKSADQYLFGWDILLYEVQSIFSNTFGSLDLALGMYPHDIVERYGSE
                     VLGVVGKNVCTLEASDVAPMVEEIDSKKILKSLSEVFTAKRCAPSKKKGNDFPTVKTD
                     IGYYTFPLGCVCCT"
     gene            complement(912..>1849)
                     /locus_tag="PK683_12625"
     CDS             complement(912..>1849)
                     /locus_tag="PK683_12625"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH09339.1"
                     /translation="GGEPTVHPDFFEVMRIAKSKPIRHIMLNTNGIKIANDPDFAKRL
                     SEFAPGFEIYLQFDSFQESALKKLRGKDLRSVREKAIQTLNELNLSTTLVVTLEKGIN
                     DSEIGEIIQFALNQKCIRGITFQPTQQAGRTENFLESGRLTLSGVRREIISQSGIFTE
                     KDLIPVPCNPDALAMAYALKLDEKVFPLTRFISPGDLLENSRNTIVFEHDERMKNHLL
                     RIFSTGNSVESLEEDFHSLMCCLPTVSAPGLKYENLFRIIIMKFMDAYDFDVRAVKKS
                     CVHMAVPDGRMIPFETMNLFYRQALLKTDMLKSGI"
BASE COUNT          531 a          368 c          388 g          562 t
ORIGIN      
        1 gacctgcgca ggagttccta cagatcagca gagcctgacc tgtgcaggag ttcctgcgaa
       61 tcagcccttg aataaacgaa aggttcgtaa tgattttaat tcccactggt tgatggtgaa
      121 cagattggtt ctgcggaaac ctaactgggt tttaactatt tgcatctctg ccgaagaata
      181 gttcaagttt ggctgctgcc atcgacacag atatttcagt ttttgttgag tattatttcc
      241 gatttcactt cctaagagtg atgatttgtt tcattccatc cagtaaaaac agtcatttca
      301 gttcagcttg acagatttct cccttttcag agaatctcca gcacacaggg aggcatttac
      361 aagtatgtgg tcaggtgcag ttacaatgta cggcatagga atgaaagaaa aagacgttgt
      421 gtcttttatt gactatcttc tgaaaaattc agagaaaaat gagaaaaagg catttttgga
      481 atatattaag agcgagtaca gtgcaaaaga ttttgaagat cttcaaaaga aaaaatcggc
      541 tgatcagtac cttttcggct gggatattct cttatatgag gtgcagtcta ttttcagtaa
      601 tacattcggt tctctggatc tggcacttgg aatgtatccg catgatatcg tagaaaggta
      661 cggaagcgaa gtattgggcg tcgtaggaaa aaatgtatgc acgttggaag ccagtgatgt
      721 ggctccgatg gtggaagaaa tagactcaaa aaaaatattg aagtccctgt ctgaagtttt
      781 tactgcaaaa agatgtgcac cttccaaaaa aaagggcaat gattttccaa cagtgaagac
      841 agatatcggg tactatacat ttcctcttgg ctgcgtctgc tgtacctgat agaaatctaa
      901 attaatttta tctatatacc gcttttcagc atatcagtct tcagcagagc ctgtcggtaa
      961 aaaagattca ttgtttcaaa cggaatcatt cgtccgtcag gcactgccat atgtacgcag
     1021 gattttttca ctgcacgcac atcaaaatca taagcatcca taaacttcat tataataatt
     1081 ctgaacaggt tctcatattt cagcccggga gcagacactg tagggagaca gcacatcagg
     1141 gagtgaaagt cttcttcaag tgattcaacc gagtttcctg tgctgaatat tctgagcaga
     1201 tgatttttca ttctttcatc gtgttcaaat acaattgtat ttctgctgtt ttcaagaagg
     1261 tcgcccggac tgatgaatct tgtcagagga aaaacctttt cgtccagttt cagagcatac
     1321 gccattgcca gtgcatcagg attgcatggg actggaatca gatccttttc tgtaaaaatt
     1381 cctgactgag aaatgatttc acgtcggaca ccggaaagcg tcagtcttcc tgattccaaa
     1441 aaattttctg ttcggccggc ctgctgagtc ggctgaaatg taattcctcg aatacatttc
     1501 tgattcagtg caaactgaat aatttctcca atctcagaat cattgattcc cttttccaga
     1561 gtgactacaa gagttgtgga cagattcagt tcgttcagtg tctggattgc cttttccctt
     1621 acagaacgca gatcttttcc tctgagtttt tttaacgcag attcctgaaa ggaatcgaac
     1681 tgaagataga tttcgaagcc gggagcaaac tcagacagcc ttttggcaaa atcaggatca
     1741 tttgctattt tgattccgtt tgtattcagc attatatgcc gtatcggttt tgatttggca
     1801 attctcatca cttcaaaaaa atccggatga acagtaggct ctcccccgc
//
LOCUS       DAOQGJ010000827         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44504_length_1849_cov_3.049052, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000827 DAOQGJ010000000
VERSION     DAOQGJ010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44504_length_1849_cov_3.049052"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="PK683_12630"
     CDS             complement(<1..1819)
                     /locus_tag="PK683_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNH09340.1"
                     /translation="MKVLNILKEGGAKIIGLTATPFHTDEKKSSRLWKLFQSPDHKGI
                     YRILKKKLIDDKILAEPYPPETVQTKIEQKEIAGDAEITRHNTSDSELKAYVLKRLGE
                     NPKRNELIAEHYKKNAEKYQKTIIFTPETMANKFLVQELKKKGIEADYVDTYRTNEEN
                     QKVMERFRTDDSLQVLVNTEMCTEGFDAPKTKTVFIARPTRSETLVQQMVGRAMRGIE
                     AGGNEKCYLVTFADTWKDFTPIDPAVVVSQAENDDLEIQKKYYQSTIPIPPEVIEECY
                     KLLHNLQIGELLSCTIEGIPYAWYEWDAPENAEDETLSDKEKVIIFETQKEGYEKLEN
                     DVKNGLLSLPEIISDPFVNSIRNKYFEVCLDPVPRPEDLKKILSAWKNSERTEDISKI
                     TFEQRDKFDTRKIAKEIIDEDLTDSKQQEKINSLWDDPVCQLLFNKNRQGFVEDINRK
                     KELLRDRQNITRELIRLKPDIQKQELKKWNAGDSGFILKEILETVGNNKKLFPKGKPN
                     IREPLFKKMSNRFFGICNYTDGNKIQISDILNSPSVPLAVMEFLMYHEMLHAAGYHRH
                     NGVFREKERLFVPSEKAMEDAKKFPEWSDTLKNSKIPWQA"
BASE COUNT          467 a          404 c          312 g          666 t
ORIGIN      
        1 gtgcctgcca gggaatcttg gaattcttca gtgtatccga ccattcggga aatttttttg
       61 catcttccat agctttttca gacggaacga ataaccgttc cttctctctg aatactccat
      121 tatgacgatg atagcctgcc gcgtgaagca tttcatgata catcagaaat tccataacag
      181 ccaaaggcac agaaggtgaa tttaagatat ccgatatctg aatcttattt ccatcagtgt
      241 aattgcagat accgaagaat cgattactca tctttttaaa gagaggttct ctgatattcg
      301 gttttccttt tggaaataat tttttattgt ttccgactgt ttccagaatt tctttcagga
      361 tgaaacccga atcaccagca ttccatttct tcaactcctg cttttgaata tccggtttga
      421 gtctgattaa ttcccgagta atgttttgtc tgtctcgcag cagctccttt ttacggttta
      481 tatcttccac aaaaccttgc ctgtttttat taaaaagaag ctgacataca ggatcatccc
      541 ataatgaatt aatcttttcc tgctgctttg aatctgtcag atcctcatct ataatttctt
      601 ttgcaatttt tctggtatca aatttatctc tctgctcgaa agtaattttc gatatatcct
      661 ctgttctttc tgaatttttc catgcagaaa gaattttttt caagtcttca ggccgaggta
      721 cgggatcaag acaaacttca aagtacttgt tccttattga atttacgaag gggtctgaaa
      781 ttatctcagg caaagacaat agtccgttct tgacatcatt ctccagtttt tcatatcctt
      841 ctttctgagt ttcaaaaata attaccttct ctttatctga taatgtttca tcctctgcat
      901 tttcaggagc atcccattca taccaggcat aaggaattcc ctcaatagta caggaaagca
      961 gctctccaat ctgcagatta tgcaaaagct tataacactc ttcaatgact tccgggggaa
     1021 tcggtatagt tgactgataa tattttttct gtatctccag atcatcattt tcggcctggc
     1081 tcactacaac ggcaggatca atcggtgtaa aatccttcca agtatctgca aaagtgacaa
     1141 gatagcattt ttcgtttccg cccgcttcaa tgcctctcat cgctcttccg accatctgct
     1201 gcacaagagt ttctgatctt gtcgggcgtg ctatgaacac agttttggtt ttaggagcat
     1261 caaatccttc cgtacacatt tcggtattta caagcacctg taaagagtca tctgtccgaa
     1321 aacgttccat tactttctgg ttttcttcat tggttctgta agtatctaca taatcagcct
     1381 cgataccttt tttcttcagt tcctgcacaa ggaatttatt cgccattgtt tcaggagtga
     1441 aaatgatggt tttttggtat ttttccgcat tttttttgta gtgttctgca atgagttcat
     1501 ttctcttagg attttctcct aatcgcttta gaacatatgc cttcagttct gagtcacttg
     1561 tattgtgtct ggtaatctca gcatcaccgg caatttcttt ttgttcaatt tttgtctgca
     1621 ctgtctccgg aggataaggt tcggccaaga tcttatcatc aatcagcttc ttcttcaata
     1681 ttctgtatat ccccttatga tcaggggact gaaacagttt ccaaagcctg cttgattttt
     1741 tttcatccgt atgaaaagga gttgcggtca agcctataat ttttgcacct ccttccttca
     1801 gaatattcag aactttcata taactgggag cctgtgcatg atgacattc
//
LOCUS       DAOQGJ010000828         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44587_length_1847_cov_3.122768, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000828 DAOQGJ010000000
VERSION     DAOQGJ010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44587_length_1847_cov_3.122768"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..763)
                     /locus_tag="PK683_12635"
     CDS             complement(29..763)
                     /locus_tag="PK683_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNH09341.1"
                     /translation="MSEILESIGVTEEIESRIKDIWISKGLSLSIPFIDLQHIWLILL
                     IVELELYQKDDAKEPEFHRTALELINYTADHFSLEENLFVRFSYPKLLPHKRQHHSFI
                     ELIKSKLSHEKLDKNERRELIEFLWTWLAEHIQKEDTDYRHFLVNSTNEYNDWFKFLI
                     ENNQITIDKAQAALYNKITNSTQVKEIISDNLYNSISSIWHMYNLSIGLPIIDLQHIW
                     LIKMVVELDYASKTLVSNKREEIFRF"
     gene            complement(920..1708)
                     /locus_tag="PK683_12640"
     CDS             complement(920..1708)
                     /locus_tag="PK683_12640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH09342.1"
                     /translation="MKKPVMLITGASRGIGEAAALLFSKDYHIILSSRTEKDLNLVKD
                     RIISLGGSADILVSDFSSVESAVKTAEEMERKFGIPDTLVLNAGISTNKDFENQTIDN
                     MVLEMNVNYLSPMAFLKTVLPGMIERKSGNIITVGSVASIIPFPGNSTYAAGKSALLS
                     MTLSLNMELKRFGIFAGCVLPGVTETDMTKAFHSVPFLPISSPKEVAECIEETLRNKN
                     EMGIPGTVNQILTAVYKAAPGPIHFILKNSLEFILPEIQKRLQN"
     gene            complement(1708..>1847)
                     /locus_tag="PK683_12645"
     CDS             complement(1708..>1847)
                     /locus_tag="PK683_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09343.1"
                     /translation="IVEIANHPWFVGVQYHPEFKSKPISPHPLFAGFIKASAAKLKEK
                     E"
BASE COUNT          520 a          361 c          369 g          597 t
ORIGIN      
        1 aacaatattc caaattaaat tcttaatgtt agaatctgaa aatttcttct cttttattgg
       61 agacaagagt cttactggca taatccagtt ctacaaccat tttgataagc cagatatgct
      121 gcagatctat aatcggaagt ccgatgctca ggttgtacat atgccagatg ctgcttatgg
      181 aattgtaaag gttgtcactg atgatttcct tgacctgtgt ggagttggta atcttattgt
      241 aaagagcggc ctgcgccttg tcaattgtga tctgattgtt ttcaatcaga aacttaaacc
      301 agtcattgta ttcattcgtg ctgttgacca gaaaatgtct gtaatccgta tcttctttct
      361 gaatatgttc cgcaagccag gtccacagaa attcaatcag ttcccttctt tcattcttat
      421 ccagtttttc gtgagaaagc tttgatttaa tgagttcgat gaaactgtgg tgctgtctct
      481 tgtgaggaag gagtttaggg taggagaacc ggacgaaaag attttcttca agagagaaat
      541 gatcagccgt gtaattgatg agttccagtg cagtcctgtg aaactcaggt tcttttgcat
      601 catctttctg atagagttcc agttcaacga tcagaagaat cagccaaata tgctgaaggt
      661 cgataaaggg tatggagagt gaaagccctt tgctgatcca aatatccttg attctggatt
      721 ctatttcttc tgtgaccccg atgctttcca gtatttctga catagtagaa actcctctaa
      781 tagtacgaat tgagatgtcc ttaataattt caaggttaat cttaataaga ctgcttctca
      841 ttttatgatt tatatcataa aagagaagtc attagaggag tgaattttat atagggtttg
      901 aaaaatatgt tagtctctgt taattctgca gtcttttctg aatctcagga agaataaact
      961 ccagagaatt tttcagtata aaatgaattg gcccaggcgc tgccttgtat acagcagtaa
     1021 gtatctgatt taccgttccg ggaatcccca tttcattttt gtttctcaat gtttcctcaa
     1081 tgcattctgc cacttccttg ggactggaga ttggaagaaa aggaactgaa tgaaatgcct
     1141 ttgtcatatc tgtttcagtg actccaggaa gaacgcatcc tgcaaatatg ccgaatcttt
     1201 tcagttccat attcagactg agagtcattg agagaagagc agacttgcct gcagcatagg
     1261 ttgaatttcc tggaaaagga atgatgcttg caacggaacc cacagtgatt atgtttccgc
     1321 tttttctttc gatcatcccg ggaagaacag tcttcagaaa agccatcgga gaaagatagt
     1381 tcacattcat ctctaaaacc atattatcaa tcgtctgatt ttcaaaatct ttattagtgc
     1441 tgattcctgc attcagaacg agcgtatccg ggattccgaa tttcctctcc atttcttctg
     1501 cagttttcac tgcagattcc acagaggaaa aatcagaaac aagaatgtcc gcacttcctc
     1561 ccagagaaat gatcctgtct ttcaccaagt taagatcttt ctccgttctc gaagaaagaa
     1621 taatatgata atccttagaa aaaagaagtg cagctgcttc accgattcct ctcgatgcac
     1681 cggttatgag catgacaggt tttttcatta ttccttctcc ttcagtttcg ctgcagatgc
     1741 cttgatgaat cctgcaaaca ggggatgagg gctgatcggt ttggacttga attcgggatg
     1801 atactggact cctacaaacc agggatgatt tgctatttcg actatct
//
LOCUS       DAOQGJ010000829         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44633_length_1846_cov_3.822446, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000829 DAOQGJ010000000
VERSION     DAOQGJ010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44633_length_1846_cov_3.822446"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PK683_12650"
     CDS             complement(<1..665)
                     /locus_tag="PK683_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09344.1"
                     /translation="MIIANPVYDSAFKYLMEDPQIAKGIIGRIIGREILEIDFKPQEY
                     ATITSSRELTFLRMDFHAIILTEENKKQKVLIELQKSKQSMNLSRFREYLGEQYAKAD
                     SIRTVKDKKGNEAEEAEFYPIIIIYLLGYIENAGLPRVVCLKNQYYDVIRNQEIVGAK
                     SEFLERLTHESYMLQLAADEVRGSTPLERLLTLFSRTDFHTKMVDYPDADEQPEDIKT
                     DNLV"
     gene            945..>1846
                     /locus_tag="PK683_12655"
     CDS             945..>1846
                     /locus_tag="PK683_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNH09345.1"
                     /translation="MKLMIIDGHALIFRAYYAFQTTGLKNSITGRPSGAVFGFFRMLF
                     KLIQDFTPSHLAVTFDPGTPLERGKIYEAYKANRAPMPEDLRPQTTEIYEICEALTLP
                     MFKIDGQEADDIIATLCRKFSKSADLIHIFSGDKDLYQILDDHIHMLRGKKGATEFIE
                     IDPKWVETEIGVKTSQIPDFMGIVGDTSDNIPGVKGIGDKGASKLIAEFGDLETIYKN
                     ISSIKNPGLVKKLEESKEMAFLSRKLATLDSDLKFDVDKKMIILPDYLKPELLEVFHI
                     RGYNVLYRDLKKISIEKGHKFKDTP"
BASE COUNT          518 a          411 c          396 g          521 t
ORIGIN      
        1 actaaattgt cagtctttat atcttccggc tgttcgtctg cgtcaggata atccaccatc
       61 ttcgtgtgaa aatccgttct ggaaaacagg gtcagaagtc tttcgagagg agtgcttccg
      121 cgcacctcgt ctgccgccag ctgcagcata taggattcat gggtcagcct ctccagaaac
      181 tcactcttgg ctccgacaat ttcctgattc ctgatgacat cgtagtactg atttttcaga
      241 cagacgactc tgggaagacc tgcattttct atatacccta agagataaat tataatgatg
      301 ggataaaatt ccgcttcctc cgcctcattc ccttttttgt ctttgacagt tctgatgctg
      361 tctgcctttg catactgctc tccgaggtat tcccgaaagc gggaaagatt catggactgc
      421 ttggacttct gcagttcaat cagcactttc tgctttttat tttcttccgt cagaataatt
      481 gcatgaaaat ccatccgaag aaaagtgagt tccctgctgg aagtaatcgt cgcatactcc
      541 tggggcttga aatcaatttc cagaatttcc cggccgataa tccgaccaat gattcctttt
      601 gcaatctgag gatcttccat cagatacttg aaggcagagt cataaactgg gtttgcgata
      661 atcataggtt tctctcatag acattttact tcgggggtgt acaaaaaagc aagaaaaaag
      721 ccgagtgcct gtacgaagcg gcacagactg tctcaccgga attgatgcaa ccgggaatgg
      781 caccgaaaat cccagacaga ctttttccct aacctgactg ccaactccac tgtggcatag
      841 ttctcgcgac cccagtgaag acttatactg aacatctggc aattttttgg agaatcagaa
      901 aacactgctt gtcagtttct aaacctctat aaatttactc ttatatgaag ctgatgatta
      961 ttgacggaca cgctctgatt ttccgggcgt actatgcatt tcagacaacc ggtctgaaga
     1021 attccattac aggaaggccg agcggtgctg tgttcggttt tttcagaatg ctgttcaagc
     1081 tgattcagga ttttacaccg tctcatctgg ctgtgacttt cgatccgggg actcctcttg
     1141 agcgaggaaa gatctacgaa gcatataagg cgaacagggc tcctatgccg gaagacctga
     1201 gaccgcagac cactgaaatt tacgagatct gcgaggctct gacccttccc atgttcaaaa
     1261 ttgacggaca ggaagcagat gacatcattg caactctctg cagaaagttt tccaaatctg
     1321 ccgatctgat tcatatcttc tccggcgaca aggatcttta tcagattctg gatgatcata
     1381 ttcatatgct tcgcggaaaa aagggagcca ctgaatttat tgagatagat cctaaatggg
     1441 tggagaccga aatcggagta aagacttctc agattcccga ttttatggga attgtgggag
     1501 acacttcaga caatattcct ggtgtaaaag ggatcggcga caaaggtgcg tccaaactga
     1561 ttgctgagtt cggcgatctg gaaacaatat ataaaaatat ttcttccata aaaaatccag
     1621 gacttgtcaa aaaactggaa gagtcgaagg aaatggcttt tctttccaga aaactggcaa
     1681 ctctcgattc ggatctgaaa tttgacgtgg acaaaaagat gatcattctt cctgattatc
     1741 tgaaacccga gcttctggaa gttttccata taagaggata caatgtattg tatcgtgatc
     1801 tgaaaaaaat ttccattgaa aagggtcata aatttaaaga tacgcc
//
LOCUS       DAOQGJ010000830         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44739_length_1844_cov_2.305757, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000830 DAOQGJ010000000
VERSION     DAOQGJ010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44739_length_1844_cov_2.305757"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..733
                     /locus_tag="PK683_12660"
     CDS             173..733
                     /locus_tag="PK683_12660"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH09346.1"
                     /translation="MTAILDLPTVKSRTHLLSIEEYHALGKLGFLSKRTELIEGVVLQ
                     KMPKSPKHATMVKKIFQFFMKSLPQGCHVISEQPITLSRSEPEPDISVISGTIEDYSE
                     SHPTTAELVMEVSLSTLAEDREMANIYAEALIPEYWLFNLNNSTVEVYIKPRNGRYSE
                     MKTYSKSETVIPKFDDSLKVSLENLF"
     gene            1398..>1844
                     /locus_tag="PK683_12665"
     CDS             1398..>1844
                     /locus_tag="PK683_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09347.1"
                     /translation="MGKDKEAFTERFLDVFRELKKLYLESEQFDRALEVSEKMRGLSV
                     SENFNLKYALSHGGVDSEKGAKLLKLKSELEALASERIVAKHQGDKGKKRENALSFRK
                     EELESYEKTEGHLEKDGFSENGRCSRTGSKNKPSGDECLRNITGNNT"
BASE COUNT          606 a          339 c          379 g          520 t
ORIGIN      
        1 cgcgcgtaag cgagctgcag gttttttaga aaaattaaaa atagagttgt tgaaattaat
       61 ttttcaacaa ctctaatact ttaaatacgt tattccttcg gttcagccta ttccgatttg
      121 aattcataaa aatgcaattg acagcattat ttcaaggttc tttgatggga atatgaccgc
      181 aattctggat ctgcctacag tcaaaagcag gactcatctt ttatcaatag aagagtatca
      241 cgctcttgga aagctgggtt ttctctcgaa aagaactgaa ttgatagaag gggtcgtcct
      301 ccaaaaaatg cctaaatctc caaaacacgc tacgatggta aaaaaaattt tccagttttt
      361 tatgaaaagt ctgcctcagg ggtgtcacgt aatttcagag cagccgatta ctctttcacg
      421 ttccgaacct gaacctgata tttccgttat ttccggaacg atagaagact attcagaatc
      481 acatccgacc acagctgaat tagttatgga ggtttcactc agtactttgg ctgaagacag
      541 ggaaatggca aacatttatg cagaagctct cattccggaa tactggctct tcaatctgaa
      601 caacagtact gtggaagttt atattaaacc taggaatgga agatattctg aaatgaaaac
      661 ctattcgaaa tcagaaacgg tcatcccaaa gtttgatgac tctctcaagg tttccttgga
      721 aaatcttttc tgatttttgg agatctgaat ttcataaagg attctatttt ctctccagtt
      781 cagatccaac ccaaaatttc ctctcagcct gaaggctctt taataaaact atgcttcggg
      841 atgttaaata gtcccgttgt catttcgatg aacagtttcc gagtctgtga agagaaatct
      901 taaagcatca ataacagaag gtcatctgca gttggaacag aatcagttaa aaaatcgtaa
      961 ctactcagcg tatgttttca tttggaattt tgatgggatt ttcaagtgcg aagcccttag
     1021 cagcttctaa gtttgcgaaa ccgccggtag gcccccgtaa aacagtaact acgaagccgc
     1081 taggctgagt agttacaaaa aatcaataca ttgcttatct gcaaaatttc taagtcgctg
     1141 tcccacctaa atcagaaaaa aatttccttg cgagcttcga tccgtaactt tctcagctcc
     1201 gccatttgaa gagtgccaat aaatccattg agttcctaca aaaatctatt cgaatatttg
     1261 aaaaatcaga agaacaagac agttatatca atgcttatat tattttgaaa aatgtctatc
     1321 ttctacaaga taagccagaa atgggcatcc attctttaaa ccaggcaatg gatctgattc
     1381 tgaaatttag atccgagatg ggtaaggaca aagaagcatt cacagaaaga tttctggatg
     1441 tattcagaga actgaaaaaa ctctacttgg aatcggaaca gtttgacagg gccttggaag
     1501 tgagtgagaa gatgagaggt ctctctgttt ctgagaattt taatctgaaa tatgctctaa
     1561 gtcatggagg cgtggattct gagaaaggtg cgaagttatt gaaactgaaa tcagaactgg
     1621 aggctctggc ttcagaaaga attgtagcga aacaccaggg agataaggga aagaaaagag
     1681 aaaatgcact gtccttcagg aaggaggaac tggaatctta cgagaagaca gaaggtcatt
     1741 tggagaagga tggtttttct gaaaatggga gatgtagcag aactggatct aaaaacaaac
     1801 cttctggtga tgagtgcctg cgaaacatca ctgggaacaa tacg
//
LOCUS       DAOQGJ010000831         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44756_length_1843_cov_3.331096, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000831 DAOQGJ010000000
VERSION     DAOQGJ010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44756_length_1843_cov_3.331096"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1843)
                     /locus_tag="PK683_12670"
     CDS             complement(<1..>1843)
                     /locus_tag="PK683_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09348.1"
                     /translation="IIEERKNFIEDKSLDRTFTGQLILKRDEWRDALHSICSDSSDEK
                     LKTVLSVLSSVTDQEKFQRMKRLGYRIMKSIPEECRDRKEPLLRKTASLLVQFRNSEI
                     SVCRSKKNTLENCSDEFGRAYPEYDILLAYVFRQLSLEVKNPVLSLYYSGNSAELLEK
                     AGGVSREVYYLPGDPLSKSARLHSRINFAGLLLDMNETAKFSEVWKESDYYAEDFADD
                     SSKFALYVLKLRELKKTGKLSFRREYLKKADSIFLNHYEVMKYADDSIVREYLKLAEE
                     IHIEDKDAESLVFRKDFYLYWKIFQAAFSAKLRFEDDAFRKSYEESFFLLKKLDETEQ
                     NLKKTFQFSMKIPELSDEKRNLNLKIRTSLLSLSEKDRSKRKILLFNEKSEISSLPSK
                     GITVFRIFEGIRESEKIISVRETDSGRIMKSFSDLRSAAEFIAESENSSDSIRMIVSE
                     KKEMETELKKLLPASEKLHYSRTVPIWNSERQISLEKTFILKPKDRLGNDVYNYNVIS
                     GRFRNVSYSNVFGESAPGFLSLKDFFLKKHFVSAVINDSSALNEQEEKMLSAVLPASS
                     VPVFIRNLQGSVPESFSRSSLKKQSSFICEGCALISDADRKADIKYIL"
BASE COUNT          476 a          384 c          366 g          617 t
ORIGIN      
        1 gagtatatat tttatatctg cttttctgtc cgcatctgaa ataagtgcac atccttcgca
       61 gatgaaagaa gactgttttt tcagacttga tctggaaaaa ctttcaggga cggaaccctg
      121 caggttcctg atgaaaacgg gtactgagga ggcaggcagg actgccgaaa gcattttttc
      181 ctcctgttca ttcaatgctg agctgtcgtt gatcactgca cttacaaaat gctttttcag
      241 gaaaaagtcc ttcagtgaaa gaaatcccgg cgcggattca ccgaagacgt ttgaatagct
      301 gacattcctg aatctgcctg aaattacatt ataattataa acatcgtttc ccagccgatc
      361 ctttggtttc agaatgaatg tcttttcaag gctgatctgt ctttcggaat tccatatcgg
      421 aacagtcctt gagtaatgca gtttttcaga tgcaggcaga agcttcttaa gttctgtttc
      481 catttctttt ttttcagaaa cgatcatcct gatgctgtcg gatgaatttt ctgattctgc
      541 aataaattct gctgcagatc tgagatcgga aaaagatttc attatccggc ctgaatccgt
      601 ttcacggact gagatgattt tttccgattc tctgattcct tcgaatattc tgaatacagt
      661 gatgcctttt gaagggaggg atgatatctc cgatttttca ttgaaaagca gaatctttct
      721 tttggatctg tctttctctg aaagagacag aagcgacgtt cttattttca gattcagatt
      781 tcttttttca tccgaaagtt cagggatttt catgctgaac tgaaacgttt ttttcagatt
      841 ctgttctgtc tcatccagtt tcttcaaaag aaagaaggat tcctcgtagg acttcctgaa
      901 agcatcgtcc tcaaatctca gttttgcact gaatgcagcc tgaaatattt tccagtacag
      961 ataaaagtct tttctgaata caagactttc agcatcctta tcttcaatgt gaatttcttc
     1021 ggcaagtttc agatattccc ttacaatgga atcgtctgcg tatttcatga cttcataatg
     1081 atttaagaat atagaatccg cctttttcag atactctctg cgaaaagaga gttttccggt
     1141 ctttttgagt tctctcagtt tcagtacata cagggcaaat ttggaggaat catctgcaaa
     1201 gtcctcagca taataatcag attccttcca tacctcgctg aatttcgctg tctcgttcat
     1261 atccagaaga agccctgcaa agttgattct ggaatgaagt cttgccgatt tgctgagcgg
     1321 atctccggga agatagtaga cctctctcga gacacctccc gccttttcaa ggagttccgc
     1381 tgaattgcct gaataatata gggaaagaac cggatttttt acttcaaggg agagctgtct
     1441 gaatacatag gcaaggagaa tgtcatattc cggataggct ctgccgaatt catcactgca
     1501 gttttccaga gtgttcttct ttgaacggca tactgaaatc tctgagtttc tgaactgtac
     1561 aaggagtgag gctgtttttc gaaggagggg ttcttttctg tccctgcact cctccggtat
     1621 ggatttcatt attctgtatc ccagtctctt catcctttga aatttttcct gatctgttac
     1681 cgaggagaga accgagagaa ctgtcttcaa tttttcatca gaactgtctg agcagattga
     1741 atgaagagca tctctccatt catccctttt cagtatcagc tggcctgtaa atgttctgtc
     1801 gagagatttg tcctcaatga aattttttct ttcctcgatg att
//
LOCUS       DAOQGJ010000832         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44793_length_1842_cov_3.407387, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000832 DAOQGJ010000000
VERSION     DAOQGJ010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44793_length_1842_cov_3.407387"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK683_12675"
     CDS             <1..483
                     /locus_tag="PK683_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09349.1"
                     /translation="FQKKLGFVPIIIRNVWTVIAIRGEIFAFSAGFFYEYGQPEIMVI
                     SDSLSSREFNLLINEICCYVRDKGPVQLGKDYTEEILIKKELELDTDPEDSDLPDSEK
                     RITARNGKEFKLINVSAPLIFNEYTRDAESSFPCEYLRSFYRNFADTEDFPMIYADLR
                     "
     gene            739..906
                     /locus_tag="PK683_12680"
     CDS             739..906
                     /locus_tag="PK683_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09350.1"
                     /translation="MKHAPQGQTATERYWKTKKTDRLNICANCENEMHQFFQLIDSEM
                     QYCNLRTGEMK"
     gene            complement(989..1663)
                     /locus_tag="PK683_12685"
     CDS             complement(989..1663)
                     /locus_tag="PK683_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09351.1"
                     /translation="MKLYTYIFLLLMNVQCTRIGMADSCLASCKRTSNSCFTASILTS
                     NMIYSNQSGASQTAIIKETEPNGNFSDAYKSSNFLGSNSISVKITISGAMSSDSDIDI
                     IEASVGGSAFKDYRFSMTGNAGCELFYSSQSFLYSSTASALDSSWNSAGSMNAPIIFP
                     YKENQSYSIYIICKGTSGSTYSVSKEDFNSGSSANSAFLNNEAYNMCHSSEKRCFAKC
                     DRGNIF"
BASE COUNT          568 a          339 c          381 g          554 t
ORIGIN      
        1 tttcagaaaa aactgggctt tgttccgatc attatccgga acgtatggac ggtgatagca
       61 atccgaggtg aaatctttgc attttcagca ggcttttttt atgaatatgg acagcctgag
      121 attatggtca tcagtgattc actgagcagc agagagttca atctccttat caatgagatc
      181 tgctgttatg ttcgggataa aggaccagtg cagctcggca aggactacac tgaggaaatt
      241 ctgatcaaga aagaactgga gttagacact gatcctgaag acagtgatct tcctgattca
      301 gaaaaaagaa ttactgcaag aaatggaaag gaattcaagc tgataaatgt aagtgcacct
      361 cttattttta acgagtatac tcgggacgca gaaagcagct ttccttgcga atatctccga
      421 tcattttacc gaaattttgc agatactgaa gattttccta tgatttatgc tgatctgcgg
      481 taaaaagaga aattcttgca gtctttcact tgagattctg cagcagtctc agcagatgga
      541 ttctgtttct cgatgaaatt tacagcagat aaaaaaaaat agtggactca gaagaatgaa
      601 gttttactag ctgaattaat taaactctga agaaagtacg gaggaaagga aatcataagc
      661 acttcaacaa cctcatcatt acactgccca atttgtggac tccctcatac tgacggacag
      721 acgaatccgg atgaatggat gaaacacgct ccgcaggggc agactgcaac tgaaagatac
      781 tggaagacaa aaaaaacgga cagactgaat atctgtgcaa actgtgaaaa tgaaatgcac
      841 cagttctttc agctcattga ttcagagatg cagtactgca atctgcggac tggggagatg
      901 aaatagatcc tatcagtcca gcttcctgta tttttgattt gcagaatttc gggaaatggg
      961 gcagtaaaag cattcctgac ggcataagtc aaaaaatatt tcctctgtcg cattttgcaa
     1021 aacagcgttt ctctgatgaa tgacacatat tgtaagcttc attgttcaga aatgctgaat
     1081 tagcggatga tcctgaattg aagtcttctt tggagacaga atatgtggaa ccggaagttc
     1141 ccttgcagat aatataaatt gaataactct gattttcttt atatgggaaa atgatcggtg
     1201 cattcattga accggcactg ttccagctgc tgtccaaggc agaagctgtg ctgctgtaaa
     1261 gaaaactctg acttgagtaa aatagctcac aaccggcatt tcctgtcata gaaaaacgat
     1321 agtctttgaa ggcagatccc cctacggaag cttcaatgat gtcaatgtct gaatctgaac
     1381 tcattgcacc gcttatagta atttttactg atatggaatt tgaacctaga aaattactgc
     1441 tcttataggc atcactgaaa tttccattcg gctccgtttc tttaataatg gcagtttgac
     1501 ttgcaccgga ttgattggaa tatatcatat tggaagtcaa tatgcttgct gtaaaacagc
     1561 tgttactggt tctcttgcag gaagccaggc agctgtctgc cattccgatc cttgtacatt
     1621 gaacgttcat taagagcagg aaaatatatg tgtataattt cattatgtgt tccttgataa
     1681 tttctactat gaatattaaa ttcttaatga ttattataaa tgtcaagaag acgaattatt
     1741 ttttgtctcc tgacgattta agatattctg caggtgttgt tacacgcttt ttttctcccc
     1801 attctgaggc agtgaaacct tgattgtttt ttgcattcac at
//
LOCUS       DAOQGJ010000833         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44806_length_1842_cov_2.569110, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000833 DAOQGJ010000000
VERSION     DAOQGJ010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44806_length_1842_cov_2.569110"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PK683_12690"
     CDS             complement(<1..265)
                     /locus_tag="PK683_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09352.1"
                     /translation="MRAKVHYLLSYTALSFYGGAVCPFIESLGWFQFSFELSCIFLLG
                     WAFRIWLSSRRKYAEIETQPRFQFIIEMSVFIFIGIFIMLFNTL"
     gene            complement(598..735)
                     /locus_tag="PK683_12695"
     CDS             complement(598..735)
                     /locus_tag="PK683_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09353.1"
                     /translation="MLWSETELGFEKEIRKLKLRMKDTEKESLKLFLPKVETIKWIAE
                     C"
     gene            1040..>1842
                     /locus_tag="PK683_12700"
     CDS             1040..>1842
                     /locus_tag="PK683_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNH09354.1"
                     /translation="MSHNTAEKLSGVSKKIDFHKVTFSKEELQTVLECLVEDLMDTGS
                     IVEKFEKDFKSTFAAKYVISANSLFSAYHLALLALGVGENDSVVLSSFAPLPAFDAIL
                     LLKAKPVVIDLGKSSFHPDPEKLKSAVDENHPKAVILDHTYGCLVDFKKYDLTNVNTI
                     EDYSESIGASYDDIPTGKQGTISLCGLSASNVITTGNGAMITTASDTIAETVKSYKYS
                     ASLRKKNKIRFDYNLLDFQAAMGIEQISKLGMIIERKKKIAQVYLQAVLS"
BASE COUNT          586 a          378 c          341 g          537 t
ORIGIN      
        1 ccagtgtatt gaaaagcata atgaaaattc caataaaaat aaatacagac atttcaatga
       61 tgaactggaa cctgggctgc gtttcaattt cagcatattt ccgccgactg ctcagccaaa
      121 ttctgaatgc ccagcccaaa agaaagatac agctcagttc aaaggaaaac tggaaccagc
      181 ctaacgactc aatgaatgga cagacagcgc cgccgtagaa agaaagtgca gtataggaca
      241 gaaggtaatg aactttggct ctcatgggaa atcagaataa aataagaatc ccagactgca
      301 agttctataa ttgaatattt caagcttgac agtattaata gaaaagtata taataaacca
      361 aggagaaaaa atatggtatt tacagatctg acttttttac tcagtattgc cggagttctt
      421 tttatgaccg gtatgggaat ctcgatcgtt tactttttca caaaaaaagc atgataaaaa
      481 aaggccattg agaattttcc caatggcctg cactgcaggg aaatatgata ttttttataa
      541 tctgcaatcc ctatttcaga accctgccga gaaggagtat caaactcgta tttgctgtca
      601 gcattccgct atccatttga ttgtttccac ttttggcaga aacagtttca aggattcttt
      661 ttctgtatct ttcattctca gcttcagttt acggatctct ttctcaaaac caagttccgt
      721 ttcgctccaa agcatattcc ttcatcgccc gttttgaaga agagagatac tccgctatct
      781 tcgcattcag ctaatagatc tcttctggag gacggatcat tttcgtaaac tgactgaatt
      841 tattgcaagt gcttcagcct gcacttcgta aacctcacct gcttccactg atcattaact
      901 gtagacccca tagtgtcctg cagaaaaatt ggagaaatcg ttaatcattt ccgccaaaaa
      961 cttaaatttt atgcatacgg tgaatgcaaa aaatccttta cacctatcgg tactttatct
     1021 aatgtgacat aagaaaaata tgtcacataa tacagcagag aaattgtcgg gtgtaagcaa
     1081 gaagattgac tttcataagg tcaccttttc caaggaagaa cttcagactg ttctggaatg
     1141 ccttgtggaa gatcttatgg ataccggttc cattgtagaa aagtttgaga aagatttcaa
     1201 atcgactttt gcggccaaat atgtaatttc tgcaaacagt cttttttctg catatcattt
     1261 ggctctgctg gcccttggtg tgggcgagaa tgattcagtt gtcctgtctt cgttcgctcc
     1321 gctgcccgca tttgatgcga ttctccttct gaaagccaaa cctgtcgtaa tagacttggg
     1381 aaaatcctca tttcatcctg atccggaaaa actgaaatct gccgttgatg aaaatcatcc
     1441 aaaagctgtc atactagatc atacttacgg atgccttgta gattttaaaa aatacgatct
     1501 tacaaacgta aatacaattg aggattattc agaatccatc ggtgccagtt atgatgatat
     1561 cccgacagga aaacagggaa caatttccct ctgtggtctc tctgcatcca atgtaatcac
     1621 taccggaaac ggtgcaatga tcacaactgc aagcgataca attgctgaaa cagtcaaatc
     1681 ctacaaatac tctgcttctc tgcgcaaaaa gaacaaaata cgttttgatt ataatcttct
     1741 ggactttcag gctgcaatgg gaattgaaca gatttccaaa ctgggaatga ttatagaaag
     1801 aaagaaaaaa attgcacagg tgtatcttca ggcggttctt tc
//
LOCUS       DAOQGJ010000834         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44885_length_1840_cov_2.638095, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000834 DAOQGJ010000000
VERSION     DAOQGJ010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44885_length_1840_cov_2.638095"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PK683_12705"
     CDS             complement(<1..1171)
                     /locus_tag="PK683_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH09355.1"
                     /translation="MEKEATVGRWQKEFFENINGFVKTGMSEADARKILEQFLYLSSI
                     TPMPPVMNVFKEPDSLEKTGVYTEMNETSRAFMIKFLNPIMRNFKVEGLENLALIEKL
                     IGKFPMTLISNHLSHLDAPGIFHLLYTSGETGRRIAEQITFIAGRLAFEPDFTRLGLY
                     MFGTLLVCSKKDMSDNPSLSDMMTKINMRAFRQSQKLQSEGKIISIFPEGTRSRDGRL
                     MPFVDTVFHYVSNKVVIPISLEGTDKILPTTGFLFNAAVGKLVIGKPVIVGDLKNKAK
                     VPEFDLLEKITPPEKGDKKQFLIDNLALLVGSNLSKHKHGSYRNLYSADKSIRENTLI
                     QIPKSPEENIVVIGSSYRSIAAAAILANKNVMVTVYTPDKEMAENCNTEQRDLEHF"
BASE COUNT          497 a          404 c          384 g          555 t
ORIGIN      
        1 gaaaatgctc cagatctctc tgttctgtgt tgcagttttc tgccatctcc ttgtccggtg
       61 tatatacagt gaccataaca ttcttgtttg caagaattgc ggccgctgca atggacctgt
      121 agcttgaacc gatgactaca atattttcct caggcgactt tggaatctgg atgagagtat
      181 tttctctgat ggatttgtct gcactgtaca ggtttctgta cgaaccgtgc ttgtgcttgc
      241 tcagattgga acctacaaga agtgccaagt tgtcaatcag gaactgcttt ttatctccct
      301 tttcaggagg agtgattttt tcgagaagat caaactcggg aaccttggcc ttgtttttca
      361 agtcgcctac gatgaccggc tttccgatca caagctttcc gactgctgca ttaaacagga
      421 atcctgtagt aggaagaatc ttgtccgttc cctccagtga aatcggaatg accactttgt
      481 tggatacata gtgaaataca gtatccacaa agggcatcag tcttccgtct ctcgatctgg
      541 ttccctccgg aaagatggaa atgatctttc cctcggactg cagcttttga gactgccgga
      601 aagcacgcat attgattttg gtcatcatat ccgagaggct cggattgtca gacatatcct
      661 tcttggaaca gacaagcagt gtaccgaaca tatacagccc gagtcttgta aaatcaggct
      721 caaaagcaag tcttcctgca atgaatgtaa tctgctctgc aatccttctg ccggtctctc
      781 cggatgtgta aagaagatga aaaatgccgg gtgcatcaag atgactcagg tggtttgaaa
      841 tcagcgtcat cggaaatttg ccgatgagct tttcgatcag tgcaagattt tccaatccct
      901 caaccttgaa gtttctcatg ataggattca gaaatttgat catgaatgcc ctggatgttt
      961 cattcatctc tgtgtagacg cctgtttttt ccagggaatc cggctcttta aaaacattca
     1021 ttaccggagg cattggagtg atcgaagaaa gatagagaaa ctgttccaga attttacggg
     1081 catcagcctc actcatccct gtctttacaa aaccgtttat attctcaaaa aattccttct
     1141 gccagcgtcc tactgttgct tctttttcca atttgctctc ctgtctctct ttttaaggtg
     1201 aagtttattt ttcagaaatg tcatttttgg tcaagaaaaa agcagtcatc tgaattcagt
     1261 gtcgactgtt tcgcgggaac gaatggaaaa tcattctgcc ctccattcac agaaatccat
     1321 accggaactc tcagtatcgg attctgtctg tcggcgttcc gatattgaag aaccagtctc
     1381 aattggaaaa tacatgcaca ttccggaaga aagagcagtc ttccgttcac tgtatttttc
     1441 cgaaagtaag cctttatttc tcgaacagaa agaaaagcag gatatcgcct gctgcagtat
     1501 ttatttctta ctgatatttc attaaaatct caactgtttt atcacagctt tcatttcagt
     1561 tccgagctca ttcaggactg cactgatcga attcaatttg cattgacagt aaaggaatgc
     1621 ctgtgtcaga ctgcaattta atccagcaga taaaaggatg caatgaaatc cttaagcaga
     1681 ccagaatgca gagtaagaat attgatgact ttcattgccg tattttctgt actgagcttt
     1741 cagaactgtc agagtgaaga agaaattaca ggcgaagagc gcgagaatgt tctgaaatac
     1801 gcagaacctg ctgctgacag catcctggca ggccataatg
//
LOCUS       DAOQGJ010000835         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_44920_length_1839_cov_3.326794, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000835 DAOQGJ010000000
VERSION     DAOQGJ010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44920_length_1839_cov_3.326794"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK683_12710"
     CDS             complement(<1..534)
                     /locus_tag="PK683_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09356.1"
                     /translation="MKSVYLGIAVLCLAGNLYSQDAVPAETPKPAEQPKPKTIELKEV
                     YEDQETGQIFSKPGDNRVKLNIAPPKQTTTLPDGFAHRPDDVSKEKFTVTGRMQFRGV
                     TGSAESLYSNGSRDYNAVDWNFRRLRLGAMYENDWWGANIQIRMENMLNRQNVVPVTS
                     TFSFTDGNGRTQNLTYVS"
     gene            complement(648..1772)
                     /locus_tag="PK683_12715"
     CDS             complement(648..1772)
                     /locus_tag="PK683_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017853470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09357.1"
                     /translation="MQANETKKQGYFNKDEKHFYLALSAVTLLMTAAYFLGMPSYYLG
                     WAAFTLAALSVAGNDAIQTVGTFIESKKQVHWLPKMAMLGLTLIAVFVYGWYIDDRQI
                     HFERLKKFDEVTEFNLIQLLAPVVLLVITRLKAPVSTTFLILGVFGGGQNIQKMLTKS
                     FVGYGVAFLAAIAVWAVLGYFDKKEYEEEHEDSEKVWSGLQWISTLFLWGAWLFQDSA
                     NIAVYLPRRLNVFELCGATGIIVLALTYIIMNGGGKIQEVVSEKSDIKSAKAGTIVDI
                     VYALILIIFQKWSNLPMSTTWVFLGLLAGRESILHIITSRDSYIDTFKKVGKDVALAS
                     LGIAVSISIFFLSQIVYPKPGQNFQILNEQKAESGVFLKK"
BASE COUNT          522 a          422 c          354 g          541 t
ORIGIN      
        1 ggatacatat gtaagattct gagttctgcc gtttccgtca gtaaaactga aagtgctggt
       61 aacaggcaca acgttctgcc ggttgagcat attttccatc ctgatttgaa tgtttgctcc
      121 ccaccagtca ttttcgtaca ttgcaccgag acgaagacgt ctgaagttcc agtccactgc
      181 attgtaatct cttgatccgt tggaataaag ggattctgct gaacctgtca cacccctgaa
      241 ctgcattctt ccagtcactg taaacttttc cttgctgaca tcatccggtc tgtgtgcaaa
      301 tccgtcaggc aaggtagttg tctgcttagg aggcgcaata ttcagcttga ctctgttatc
      361 tccaggtttg gaaaaaatct gacctgtttc ctgatcctca tatacttcct tgagctcgat
      421 ggttttcggc ttcggctgtt cggccggttt cggcgtctcc gcaggaactg cgtcctgaga
      481 gtaaagattt cccgcaaggc aaagcacggc tatgcccaaa taaactgatt tcataaatat
      541 cctctgtttt attattttat gacattttac acattatact tataaataaa tataatgtat
      601 cttatttgct tcggaagacc tgctttcata agtcttccaa agataattca ttttttcaaa
      661 aaaactcctg attctgcttt ctgctcattc agtatctgaa aattctgtcc cggtttggga
      721 tacacaatct gtgacaggaa gaagattgaa atggagactg cgattccaag agaagcaagt
      781 gcgacgtcct ttcctacttt cttgaaagtg tcaatataag agtctctgga tgtaataata
      841 tgcagaatag attcgcggcc agcaagaaga cccagaaaca cccatgtagt ggacatcggg
      901 agattgctcc atttctggaa aataatcagt atcagtgcat atactatgtc cacaattgtt
      961 cctgcctttg cagacttaat gtccgatttt tcagaaacaa cttcctgaat ttttcccccg
     1021 ccgttcatta taatatatgt cagagccaga acaatgattc cagtcgcacc gcaaagttcg
     1081 aatacattca gtcttctggg aaggtagacg gcaatattgg cagaatcctg aaaaagccat
     1141 gcaccccaga gaaacaacgt tgaaatccac tgaagcccgg accatacttt ttcactgtcc
     1201 tcatgttctt cttcatattc ttttttatca aaatatccaa gaactgccca gactgcaatt
     1261 gccgcaagaa atgcaactcc atacccgaca aacgatttag tcagcatttt ctggatattc
     1321 tggcctcctc caaaaacacc cagaatcaga aatgtagtgg agacgggagc tttcagcctg
     1381 gtaattacaa gcagcacaac cggtgcaaga agctgaatga gattaaactc agtcacctca
     1441 tcaaattttt tcagtcgttc aaaatgaatc tgtctgtcgt caatgtacca cccataaaca
     1501 aaaactgcaa tgagtgtcag tccaagcatt gccattttgg gaagccagtg aacctgcttt
     1561 ttactctcaa taaaagttcc gactgtctgt attgcgtcat ttcctgcgac agagagtgcg
     1621 gccagggtaa aagctgccca gccgagatag taggacggca ttcccagaaa gtatgccgct
     1681 gtcatcagta aagtcactgc tgaaagagcc agataaaaat gcttttcatc cttgttgaaa
     1741 tagccttgct ttttcgtttc atttgcttgc atactgtaac catgtcgtaa ctatgttaca
     1801 aaatgattac agaacatgaa atatctggtt acatattac
//
LOCUS       DAOQGJ010000836         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45001_length_1837_cov_3.713244, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000836 DAOQGJ010000000
VERSION     DAOQGJ010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45001_length_1837_cov_3.713244"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1273)
                     /locus_tag="PK683_12720"
     CDS             complement(32..1273)
                     /locus_tag="PK683_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNH09358.1"
                     /translation="MKKILIIFFAAELVWKLVLKYLNYEGDSSPETRARILKFFTEAD
                     ITVGQEYSRSGFYAGLGSFAVKNILMFFMAFSPLSARLEDIVKKWSRDREWLTNMLFV
                     LAAYTVMTVLFLPLDFYFSYVLEHKFGFSNMTVGAWFWRECKNFILGAVFMTIAVLPS
                     LWIIKKVQFYWIFIVPTAGIIFGLFMSVVYPVLILPVYYDVEKIQEGSLKTKIMKLAD
                     SSKIPVSEIFIIKESEYSNHTNAFFVGFGDNKKIYLYDTLIKKNSEEETISVLAHEIG
                     HWKYNHQIKGIVYEFIFSLLEFGLLYFCLKKLKEETDLIREFHSVSSLPMFILIFGLV
                     STVLSPVNAGLSRSMEAEADYEALVLTDDPESFISSEIRLARDNKSRLDSHPLPSFIY
                     DSHPETLDRIEMAVKYRERKK"
     gene            complement(1273..>1837)
                     /locus_tag="PK683_12725"
     CDS             complement(1273..>1837)
                     /locus_tag="PK683_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNH09359.1"
                     /translation="EGREVEEPFGKGQKGSSAMPHKRNPVICERISGISRVIRSNALV
                     ATQNMPLWHERDISHSSAERIILPDSTIALEYILERMNYVVKNMHIYPEASLRVMDVT
                     RGLFYSQKAMLQLIEKGGFTREKSYAIVQKHAMDVWADIHASLKEKLLKDPEVSSVMK
                     KDDIDEIFQVEPYLKRVDFVYRRLGLK"
BASE COUNT          549 a          421 c          333 g          534 t
ORIGIN      
        1 ggcacttaca gccgcagtat gccgaaagac attacttttt tctctctctg tatttcactg
       61 ccatttcaat cctgtccaat gtttcaggat ggctgtcgta aataaatgaa ggaagaggat
      121 gagaatccag tctggactta ttgtctctgg caagccggat ttctgaactg atgaaggatt
      181 ccggatcatc agtcagaaca agtgcttcat aatccgcctc tgcttccatt gatcttgaaa
      241 gcccagcatt gaccggagat aaaaccgtcg agaccagacc gaaaatcagg atgaacatcg
      301 gaagagaaga tactgaatga aactcccgga tcagatcagt ttcctctttc agctttttca
      361 gacagaaata gagcagcccg aattccagaa gagagaatat aaattcatat acaattcctt
      421 tgatctggtg attgtatttc caatgcccaa tctcatgtgc aagaacagaa attgtctcct
      481 cttccgagtt tttctttatc agagtgtcat aaagataaat ctttttattg tctccgaacc
      541 ctacaaaaaa tgcatttgta tgattggagt attcactctc cttgatgatg aaaatctccg
      601 atacaggaat ctttgaagaa tctgcgagtt tcattatttt tgtcttcaga gaaccttcct
      661 gaattttttc cacatcataa tagacaggca gaatcagcac tggatacacg acactcataa
      721 aaagaccgaa tatgattcct gcagtcggga caatgaatat ccagtaaaac tgcacctttt
      781 tgatgatcca gagtgaggga agaacagcaa ttgtcataaa cactgccccc agaataaaat
      841 tcttgcactc cctccagaac caggcaccta cagtcatatt cgaaaatccg aacttatgct
      901 caaggacata ggaaaaataa aaatccaagg gaagaaaaag aacagtcatt acagtatatg
      961 ctgccagtac aaaaagcata ttggtcagcc attccctgtc cctgctccat ttctttacaa
     1021 tgtcttcaag ccttgcagaa agaggtgaaa atgccatgaa aaacataagt atgttcttca
     1081 ctgcaaatga acccagtcct gcataaaaac cgcttctgga atactcctga ccgactgtta
     1141 tgtccgcttc agtaaaaaat ttcagaatcc ttgccctggt ttcaggagaa gaatctcctt
     1201 catagttcag atacttcaga acaagcttcc atacaagttc tgcagcaaaa aatataatca
     1261 gtattttttt cattatttca gccccagccg tctgtataca aagtccactc ttttcaaata
     1321 aggttccacc tggaagattt cgtcaatgtc atcttttttc attacagaag acacttcagg
     1381 atctttcaga agtttctctt tcagacttgc atgaatgtcc gcccacacat ccattgcatg
     1441 tttctgaaca attgcatagg atttctctct tgtaaatccg cccttctcta tgagctggag
     1501 cattgccttc tgagaataaa aaagtcctct tgtaacatcc atcacccgaa gggatgcctc
     1561 agggtagata tgcatattct tcaccacata gttcattctt tccagaatat attccagtgc
     1621 tatcgtagaa tcaggaagaa tgattctttc tgcagaggaa tgtgagatgt ctctttcatg
     1681 ccagagaggc atgttctggg tggcgaccag tgcattagaa cgtatcactc ttgagatacc
     1741 ggatatcctt tcacagatga ccggatttct cttgtgaggc attgcggaag atcccttctg
     1801 tcctttcccg aaaggctctt cgacttctct tccctct
//
LOCUS       DAOQGJ010000837         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45104_length_1835_cov_2.808989, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000837 DAOQGJ010000000
VERSION     DAOQGJ010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45104_length_1835_cov_2.808989"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PK683_12730"
     CDS             <1..348
                     /locus_tag="PK683_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09360.1"
                     /translation="TNVIGRKIIMNSDGEKIIHYYINSNLYGVFNNIVFDKANPQFEL
                     LKPIPEGEPLYKSVIFGQTCDSMDKIADGIMLPELVCGNWLIIRNHGAYTIASASRFN
                     GFELEDVNYVFTF"
     gene            358..1461
                     /gene="nspC"
                     /locus_tag="PK683_12735"
     CDS             358..1461
                     /gene="nspC"
                     /locus_tag="PK683_12735"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNH09361.1"
                     /translation="MNIKTPYYLIDENRLLKNLKKMETLRGLSGAKTVLALKCFSTWS
                     VFELMKNYMDGTTSSSLYEARLGYEKFGKEVHAYSVAWSEKEILEVRDFSTKILFNSV
                     SQLKRFLPLVKGKNLGLRINPGISHSGYDLADPARKYTRLGVSEISSADEVLQEISGV
                     MFHCNCDNDDYQSFSKILDCISDRYKDLLEKMEWVSLGGGIYFTKDSYPFEEFCEKIR
                     KFSEKFKVQVYLEPGETAITMSGFLITEVLDIVQNEIEIAIVDASVETHMPDLLIYRL
                     DAKMEIPKGGKHRYMIAGRTCLAGDIFGTYDFPEKLKVGDRITVADAAGYTMVKKNWF
                     NGIQMPGIAVQRLDGRIELIKEFTYEDFLNGLS"
     gene            1475..>1835
                     /locus_tag="PK683_12740"
     CDS             1475..>1835
                     /locus_tag="PK683_12740"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNH09362.1"
                     /translation="MKKNVLIIGAGGVAHVAAHKCAQNNDVLGDICIASRKLEKCEKI
                     IESIKRKGNIKDSSKKLYARQIDAFDVPATVQLIKDTKSEIVINLGTAYVNMSVLEAC
                     LEAGVSYMDTAIHEDPDK"
BASE COUNT          601 a          348 c          423 g          463 t
ORIGIN      
        1 acaaatgtga tcggaagaaa aatcattatg aattccgacg gagaaaaaat catacattat
       61 tatatcaatt ccaatcttta cggggtgttc aacaatatcg tatttgacaa ggccaatcct
      121 cagtttgaac ttctgaaacc gattcctgag ggagaaccgc tttacaaatc ggtcattttc
      181 ggacagacct gcgacagtat ggacaagatt gcagacggaa tcatgcttcc tgaacttgta
      241 tgcggaaact ggctcatcat ccgaaatcac ggtgcatata caattgcatc tgcatccagg
      301 ttcaacggat ttgaactgga agatgtgaat tatgtattca ccttttaata gaatgcaatg
      361 aatataaaaa caccatatta tctgatagat gaaaaccgcc ttctgaagaa tctgaaaaaa
      421 atggaaactt tgcgcggtct ttccggagcc aaaaccgtgc tggcactgaa atgtttttcc
      481 acctggtcgg tatttgaact gatgaaaaat tacatggatg gaaccacttc aagctcgctc
      541 tatgaagcca gactgggata tgagaaattc ggaaaggaag ttcacgccta cagcgtggcc
      601 tggtcggaaa aagaaatcct tgaggtgcgg gatttcagca ctaagatcct tttcaattca
      661 gtgtcccagc tgaaaagatt tcttcctctg gtaaaaggaa aaaatctggg gctcagaatc
      721 aatccgggaa tcagccattc aggatatgat ctggccgatc ctgccagaaa atatacaaga
      781 ctcggagtgt cggaaatatc ctcagcagac gaagttctgc aggaaatatc aggggttatg
      841 tttcactgca actgtgacaa tgacgattat cagtcttttt ctaaaattct agactgtata
      901 tcggacagat ataaagatct tttagaaaaa atggaatggg tcagccttgg agggggaata
      961 tacttcacaa aggacagtta tccttttgag gaattctgtg aaaagatcag aaagttttct
     1021 gaaaaattca aggtgcaggt atatctggaa ccgggagaaa ctgccattac aatgtcaggt
     1081 tttttaatta cagaggttct cgacatagtt cagaatgaaa tcgaaattgc aattgtggat
     1141 gcttcagtgg agacccatat gccggatctt ctcatctaca gactcgatgc taagatggag
     1201 atcccaaaag gcgggaaaca caggtacatg atcgcgggaa gaacctgtct tgcaggagac
     1261 atattcggaa cctatgattt cccggaaaag ctcaaagtcg gagacagaat cacggttgcg
     1321 gatgccgcag gctatacaat ggtgaaaaaa aactggttca acggaattca gatgcctgga
     1381 atcgctgttc agaggcttga cggcagaatc gaactcataa aagaatttac atatgaggat
     1441 tttctaaacg gtctttcata aagggaggag aaaattgaaa aaaaacgtac tgataatagg
     1501 tgccggaggt gttgctcatg tggcggctca taaatgcgct cagaacaatg atgtgctcgg
     1561 agacatctgc attgcttcga gaaaactcga aaaatgcgag aagatcattg agagcataaa
     1621 aagaaaggga aacatcaagg acagttccaa aaaactttat gccagacaga ttgatgcatt
     1681 cgatgtgcct gccacagtgc agctcatcaa ggatacaaaa tcggagattg taattaattt
     1741 aggcactgct tacgtgaata tgtcagtcct tgaggcatgt cttgaggcag gagtttccta
     1801 tatggacacc gcaatccatg aagatcccga caagg
//
LOCUS       DAOQGJ010000838         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45201_length_1833_cov_2.848706, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000838 DAOQGJ010000000
VERSION     DAOQGJ010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45201_length_1833_cov_2.848706"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..863
                     /locus_tag="PK683_12745"
                     /pseudo
     CDS             1..863
                     /locus_tag="PK683_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004767629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            923..1351
                     /locus_tag="PK683_12750"
     CDS             923..1351
                     /locus_tag="PK683_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09363.1"
                     /translation="MTAIVTLILILSYVIFRQFLFDRRKIHQKGIIDRISLCVIHPDT
                     ILPEVEIFFKYYFGGGVYKGKGYCLLTDFLSDTDYRIEFNAFLEPILFIGDKVFVSME
                     HIESYLLSSIDTVILEIDPVEPYRVKLLGLNEESQGAKKI"
     gene            1335..1721
                     /locus_tag="PK683_12755"
     CDS             1335..1721
                     /locus_tag="PK683_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016759167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNH09364.1"
                     /translation="MQKKSDCKICLIHSSENSPILFESKNFFTRKAEPEKNCPGYLYI
                     EPKKHIENLTEFTSDEFTEFGLLMEKSVRWIYANFKPLKLYTVTVSEAVPHIHWHLVP
                     RYSDELKGFAYIQSALEGRLPEINAF"
BASE COUNT          574 a          331 c          416 g          512 t
ORIGIN      
        1 gggcggtcag aagggccaga acaggcttta aaaacgaaaa gagacctaca ggttcattta
       61 tttttatggg gcctacagga gtgggaaaga cagagcttgc aaaggcactg gcaacattcc
      121 tcttcggaag ggacgatcat ctgcttagag tggatatgtc cgagtatatg gaacctcatg
      181 cagtcagcag actcattggt gctcctccgg gatatgtagg atatgatgac ggcggacagc
      241 tgactgaatt tgtcagaaga aagccatact cagttgttct tctggatgaa attgaaaagg
      301 cgcacccgga tgtattcaat attcttcttc agattatgga agagggaaac ctgacagatt
      361 cgaagggaag gaaagtcaat ttcagagata caatcgtcat tatgacttcg aatatcggtg
      421 ccaagctgat tcagaagggc ggaagactcg gatttgagga ttcggatgaa aaagacaagc
      481 agaagtcaga gatgaccaag gatgagctga aaaagtattt caatcctgaa ttcctgaaca
      541 gagtggacga aatcatttat ttccagcccc tctccaaaga agagatcatt cagattgtgg
      601 acattatgct taaggatttc aacaagcgtc ttgttcagaa gaagatttcc gtagtgatga
      661 cagatgacgc caaggactat atttctgaaa tcgggtatga taagaattac ggtgctaggc
      721 ctctcagaag agttttccag aaggaaattg aggactatat ggcagttcag atgctgaatg
      781 gtgcatataa gatgcctaca ctgattcaaa tggacagcaa agacaagaag tttgttttct
      841 ctgaacgtcc ttgggaagat ttcggtctcg aagaggttca ggagaacaag gactccggcg
      901 aggaaaaaca agtgatcact gtatgacagc gatagttaca cttatactga ttttatccta
      961 tgtaatcttc agacagtttc tgtttgacag acgaaagatt caccagaagg gaatcataga
     1021 tagaatcagt ctttgtgtaa ttcatccgga tacaatcctt cccgaagtag agatcttttt
     1081 taaatactat ttcggaggag gagtttacaa ggggaagggg tactgtcttc ttacggattt
     1141 cctctctgat acagattaca ggattgagtt taatgcattt ctcgagccca ttctgttcat
     1201 tggcgacaag gtatttgtat ctatggaaca tattgaatcc tatcttcttt catcaataga
     1261 tacagtcatt ctcgagattg atccggtgga accctacaga gtgaaacttt tgggattgaa
     1321 tgaagagtct caaggtgcaa aaaaaatctg actgtaaaat ctgcctgatt cacagttcag
     1381 agaattcacc gattctcttt gagtcaaaaa atttttttac aagaaaagcg gaaccggaaa
     1441 agaactgtcc gggttatctt tatattgaac ctaaaaagca tatcgaaaat cttactgaat
     1501 ttacttctga cgaattcact gaattcggtc tgctgatgga aaaatcagtt cggtggattt
     1561 acgcaaattt taaaccttta aaactttata ctgtcacagt gtccgaagca gtcccccata
     1621 ttcactggca ccttgtgcca agatactcag atgaactcaa aggatttgcc tatatccagt
     1681 ctgcacttga aggcagactt cctgaaataa atgcattctg atcccggcag atttattttc
     1741 ctggatcggg atggagtcat caatgaaaga aattttgaag gatatattct ctcttatgag
     1801 gatttccatt ttaaaaagga tgcatttgaa agt
//
LOCUS       DAOQGJ010000839         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45240_length_1832_cov_2.904333, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000839 DAOQGJ010000000
VERSION     DAOQGJ010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45240_length_1832_cov_2.904333"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PK683_12760"
     CDS             complement(<1..437)
                     /locus_tag="PK683_12760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNH09365.1"
                     /translation="MNYIIFDLEATCEKNIKLPDSEIIEIGAVRLDKDLKETGRFCRF
                     IRPVYRPVLSEFCKRLTTIKQSETDSAEIFSEVLPAFEDWIEETGPARLYSWGGYDKR
                     QILRESKMKNYSGKIQRLLENHFNLKEEYQKIYNHSYGSGMDSV"
     gene            complement(502..>1832)
                     /locus_tag="PK683_12765"
     CDS             complement(502..>1832)
                     /locus_tag="PK683_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNH09366.1"
                     /translation="KTASVKLPEGPFCGVPFLLKDLLAAYAGEPMNSGSKAYRNYIPL
                     EDSELVRRFKNAGTVILGKTNTPEFGLMGVTEPELHGPTLNPWDTARTPGGSSGGAAA
                     AVSSGMVPIASAGDGGGSIRIPASNCGVFGLKPSRGRNPTGPIYGELWQGAAVEHIIS
                     RSVRDSAAMLDFTCGDDSGSPYPVSRPEKPYSSLLGRNGPRLRIAFSSASPVGRGVHN
                     DNISALNDAVSLLQSLGHKVEEKHPDIDGNELAMSYLTMYFGETAEEIRAAGKILGRK
                     PCRSDFEGTTWLLGLLGRVYTAGDFASAMKVWNRLGRIMGKFHETYDLYLTPTLAYPP
                     VKIGELAPKPGEKIAMDIVNMFGLGKLMKATGMVQELALKSLEKSPFTQLANLTGQPA
                     MSVPLYWSKENLPIGVQFIAPMGREDVLFQLAAELERARPWFDKRPPEIQ"
BASE COUNT          432 a          460 c          395 g          545 t
ORIGIN      
        1 acagaatcca ttcctgaacc gtaagaatga ttatatatct tctgatattc ttccttcaga
       61 ttaaaatgat tttccagaag tctttgaatt ttcccgctgt agtttttcat ttttgattcc
      121 ctcagaatct gtcttttatc atagccgccc caggaataga gtcttgccgg ccctgtttcc
      181 tcaatccagt cttcaaatgc aggcagaact tccgaaaaaa tctcagcact gtcagtctca
      241 ctctgtttta tcgttgtcag tcttttgcag aattcactta agactggtct gtatacaggg
      301 cgaatgaatc tgcagaatct tccagtctct ttcagatcct tgtccagccg tactgctccg
      361 atttcaatga tctcggaatc aggcagtttt atgttttttt cacaggttgc ttccagatca
      421 aatatgatat aattcattat aatttccttt atcgtttttt atgaatggat attgtcatag
      481 ggacaggtct gagacctgtc tctactgtat ttccggaggt cttttgtcaa accagggacg
      541 tgctctttcg agttctgctg caagctgaaa aaggacatcc tctcttccca taggagcaat
      601 aaattgtaca ccgattggga gattttcctt tgaccagtac aagggcactg acattgccgg
      661 ctgtccggtc agatttgcca gctgagtgaa gggagatttc tccaggctct ttaaagccag
      721 ttcctgcacc atccctgtcg ctttcatcag ttttcccaga ccgaacatat ttacaatgtc
      781 catagcaatt ttctcaccgg gcttaggcgc aagctccccg attttcacag gagggtatgc
      841 gagagtaggg gtgagataca gatcatacgt ctcatgaaat tttcccataa ttcttcccag
      901 cctgttccat accttcattg cactggcaaa atcaccggca gtatagactc ttcccagcag
      961 cccaagaagc caagtcgttc cctcaaagtc tgatctgcag ggttttctgc ccagtatttt
     1021 ccctgcagct ctgatttctt ccgctgtctc gccgaagtac attgtaagat aactcattgc
     1081 aagttcattt ccgtctatat cgggatgttt ttcttccact ttgtgaccca ggctctgaag
     1141 gagggatact gcatcattta gtgcggatat attatcatta tgaactcctc ttccgaccgg
     1201 cgaagctgaa gagaaggcga ttcgaagcct tggaccgttc ctgccgagca gagatgaata
     1261 aggcttttcc ggtctgctta ccggatatgg ggaaccggaa tcgtcgccgc aggtgaagtc
     1321 aagcatggca gcactgtctc tgaccgatct gctgatgata tgctcgacag cagctccctg
     1381 ccagagttct ccgtaaatcg gtcctgtagg atttcttcct cggctgggct taagtccgaa
     1441 tacaccgcag ttggatgcag gaatgcgaat agaaccgcct ccgtctcctg cagacgcaat
     1501 gggaaccatt cccgatgaaa cggctgcggc tgcccctcca ctcgatcctc ccggagttct
     1561 ggccgtgtcc caaggattca atgtcggtcc gtgaagttca ggctcagtga ctcccataag
     1621 gccgaattca ggagtatttg tttttcctaa tatgacagtt cctgcatttt tgaatcttcg
     1681 taccagttca gagtcctcaa gagggatata atttctatat gctttgcttc cgctgttcat
     1741 cggctctcct gcataagcgg ccagaagatc tttcaaaaga aaaggaacgc cgcagaaggg
     1801 accctcagga agcttgacgg atgcagtttt cc
//
LOCUS       DAOQGJ010000840         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45366_length_1829_cov_2.788050, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000840 DAOQGJ010000000
VERSION     DAOQGJ010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45366_length_1829_cov_2.788050"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="PK683_12770"
     CDS             <1..110
                     /locus_tag="PK683_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNH09367.1"
                     /translation="RHRKGLPVRGQRTRTNARTRKGVKKTVANKKKVTK"
     gene            123..530
                     /gene="rpsK"
                     /locus_tag="PK683_12775"
     CDS             123..530
                     /gene="rpsK"
                     /locus_tag="PK683_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNH09368.1"
                     /translation="MAEKEKDKKTKKVKKREKKNIPRGKVYIQASFNNTIVTVTDMAG
                     NTISWSSSGLMAFKGSKKSTPYAAQVAATNAAEKAIDIAGIREVDVLVSGPGIGRESA
                     IRSLASKGLSIKMIKDITPLPHNGCRPRKRRRV"
     gene            546..1178
                     /gene="rpsD"
                     /locus_tag="PK683_12780"
     CDS             546..1178
                     /gene="rpsD"
                     /locus_tag="PK683_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNH09369.1"
                     /translation="MARYTGPVCKLCRREGLKLYLKGSRCFNKEKCSFEDRKNPPGLP
                     PKKKGKITDYSLQLREKQKVKRVYGVLEKQFRDYFEKANHMEGITGELLLQLLERRLD
                     NVVFRLGFASSRAQSRSMIAHGHITVNGAKVDIPSYQIKIGDKISFKEKMMKSPTLDQ
                     NIKFAQSMNRHPSWLTPDYSSFTGEVTAMPAREHIDIPVKEQVIVELYSK"
     gene            1196..>1829
                     /gene="rpoA"
                     /locus_tag="PK683_12785"
     CDS             1196..>1829
                     /gene="rpoA"
                     /locus_tag="PK683_12785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNH09370.1"
                     /translation="MMHKNLLKGFKRPKKIEYFTETSEPNYGKFTAEPFERGFATTVG
                     NSLRRTLMSSIEGAAISAIRIDGVTHEFSIIEGVVEDVPRIILNLKQVFVKYEPEDRD
                     QNKVIHVELKGPGYFRAGDLAVDSSIEIMNPDLIIATLNEDANVIMDLEIQRGKGYIP
                     AEERKKDIEVLGTIPIDSLFSPVTKVLFEISETRVVQRSDYEKLTLEIWTN"
BASE COUNT          634 a          310 c          404 g          481 t
ORIGIN      
        1 ttcgtcacag aaaagggctc cctgttcgcg gacaaagaac aaggactaac gccagaactc
       61 gcaaaggtgt taagaaaaca gttgcgaaca agaagaaagt aactaagtaa actaggtgaa
      121 tcatggctga gaaagaaaaa gacaagaaga ccaaaaaggt caagaaaaga gaaaagaaga
      181 acattccgag aggaaaagtt tatattcaag cttctttcaa caacacaatt gtgactgtaa
      241 ctgatatggc tggaaacaca atctcctggt catcttcagg actcatggca ttcaagggtt
      301 caaaaaagtc cactccatat gcagctcaag ttgcagcgac aaatgcggct gaaaaagcaa
      361 tcgatattgc aggaatcaga gaagtggatg ttctagtttc cggtcctggg atcggaagag
      421 aatctgcgat cagatcactt gcatctaagg gactttcgat caagatgatc aaagatatta
      481 ctcctctgcc tcataatgga tgcagaccga gaaaaagaag aagagtttga taggggaatt
      541 ataggatggc aagatataca ggtccagttt gcaaactttg cagaagagaa ggattaaaac
      601 tttatttgaa aggaagtcgt tgtttcaata aggaaaaatg cagttttgaa gacagaaaaa
      661 atcctccggg tcttccgcca aagaagaaag gaaaaattac tgattactct cttcagctca
      721 gagaaaagca aaaagtcaaa agagtgtatg gggttcttga aaaacagttc cgtgactatt
      781 ttgaaaaggc caaccatatg gaaggcatca caggtgaact tcttcttcag cttttagaaa
      841 gaagactgga taatgttgtg ttcagacttg gatttgcgtc ttcaagagct cagtccagat
      901 ctatgattgc tcatggacac attactgtga acggtgcaaa agtggatatt ccatcatacc
      961 aaatcaagat tggtgataaa atttctttta aagaaaaaat gatgaaatct cctactctgg
     1021 atcagaatat caagtttgct caatctatga atcgtcatcc tagctggctt actcctgact
     1081 attccagttt tactggagaa gtgactgcaa tgcctgcaag agaacacatt gatatccctg
     1141 ttaaagagca ggttatcgta gaattgtatt ctaaataatt agaaggattt cgagaatcat
     1201 gcataagaat ctactgaaag gtttcaaaag acctaaaaag attgaatact ttactgaaac
     1261 cagtgaaccc aactatggaa agtttactgc agaacctttt gaaagaggtt ttgcaacaac
     1321 agtaggaaat tcacttcgca gaactctgat gtcctcgatt gagggggctg caatttctgc
     1381 cattcggatt gatggagtga ctcacgaatt ttcgattatc gaaggggtcg tggaagatgt
     1441 tccgagaatc attctgaatc tgaagcaggt atttgtaaag tatgagccgg aagacagaga
     1501 tcagaataaa gtaattcatg ttgaattgaa aggtcctgga tattttcgtg caggagactt
     1561 ggcagttgat tcttccattg aaattatgaa tcctgatctg atcattgcaa ctttaaatga
     1621 agacgctaat gtgatcatgg atttggagat tcaaagaggc aagggctata ttcctgctga
     1681 agagagaaaa aaggacatcg aagtgcttgg aactattcca atagattctt tgttctctcc
     1741 tgtaactaaa gtgcttttcg aaatcagtga aactagagtc gttcaaagat ctgattatga
     1801 aaaacttact ttggaaatct ggactaacg
//
LOCUS       DAOQGJ010000841         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45395_length_1828_cov_4.010716, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000841 DAOQGJ010000000
VERSION     DAOQGJ010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45395_length_1828_cov_4.010716"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1828
                     /locus_tag="PK683_12790"
     CDS             19..>1828
                     /locus_tag="PK683_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNH09371.1"
                     /translation="MPEVYSELVNICKKLEHHYKNVQDIEFTVEKGKLWILQTRDAKR
                     TAQAAVRIAVEMAEEWTITKEQALLRVTPEQIDFFLHPQFKSEERKKATAAGLMIAKG
                     LNVSPGAAVGQVAFDADLAVKWAAEGRKVIMVRPETKPDDVHGMLAAKGILTSRGGRT
                     SHAALVARQFGKPAIVGVTTLEIDLLHRRMHVHDNIINEGDIISIDGNTGEVFIGEIS
                     TVVPDIKDPYLIRLLEWADEIRKLEVWANADYPVDAQRARDNGAQGIGLCRTEHMFFQ
                     TERLPFVQKMILSKEEASRQEALNALLPFQRNDFAGLFRVMNGLPVVIRLIDPPLHEF
                     LPSISDLQLELSDLKIKLLREASNLDQVNKLLEKISERKALLEQVESMHEQNPMLGLR
                     GVRLGIHLSALTRMQVRAIIEAACIVMREGIVCYPKIMIPLTSHVNELKLQQTTLEDE
                     AKQVMQEMGMSLQYKFGTMIEIPRAAMTADELAEYASFFSFGTNDLTQTTYGISRDDA
                     EKGFLIEYLEKGILKDNPFATIDRYGVGKIMKIAVDLGRKARSDLEIGICGEHGGEPL
                     SVHFCHEIGLNYVSCSPFRVPIARLAAAQIVLKEKGV"
BASE COUNT          577 a          373 c          457 g          421 t
ORIGIN      
        1 catcagctga gaaaagaaat gcctgaggtc tacagtgagc tggtgaatat atgcaaaaaa
       61 ctggaacatc attataaaaa cgtacaggat attgaattta cagttgaaaa gggaaaactc
      121 tggattcttc agacgagaga cgcaaaaaga actgctcagg ccgcagtgag aattgccgtg
      181 gagatggcgg aagaatggac aattacaaaa gagcaggctc ttcttcgtgt aactcctgaa
      241 cagatcgact ttttccttca tccccagttc aagtctgaag aaagaaagaa ggcaactgca
      301 gccggactga tgattgcaaa aggactgaat gtttctcccg gagccgcagt aggacaggtg
      361 gcatttgatg cggatcttgc agtcaagtgg gcagccgagg gcagaaaagt catcatggtc
      421 agaccggaaa ccaagccgga tgacgtgcac ggaatgctgg ctgcgaaagg aatccttacg
      481 agcaggggag gaagaacaag tcatgctgcg ctggttgcaa gacagttcgg aaaacctgcc
      541 atagtcggag tgacgacact ggaaattgat cttcttcacc gaagaatgca tgttcatgat
      601 aatataatta atgaaggaga tattatatcc attgacggaa acactggaga ggtgttcata
      661 ggagaaattt ccacagttgt tcctgacatc aaggatcctt atctgatccg tcttctggaa
      721 tgggcagacg aaatcagaaa actggaagtc tgggcaaacg cagactatcc tgtagatgca
      781 caaagagcaa gagacaacgg tgctcagggg atcggtctct gcagaacaga gcatatgttc
      841 ttccagactg aaagacttcc ttttgtgcag aaaatgattc tctccaagga agaagcaagc
      901 agacaggaag cattgaatgc acttcttcct ttccagagaa atgattttgc aggactgttc
      961 cgtgtaatga acgggcttcc tgtagtcatc cgtctcattg accctcctct gcatgagttc
     1021 cttcccagca tttccgatct tcagctggaa ctttcggatc tcaaaatcaa acttctcaga
     1081 gaggcatcca acctggatca ggtgaacaaa cttctggaaa aaatttccga aagaaaggca
     1141 cttctcgagc aggtagaatc catgcatgaa cagaacccga tgctcggtct tcgcggtgta
     1201 agactcggaa ttcatctgag tgcactcaca agaatgcagg tgagggccat cattgaagct
     1261 gcctgcattg tgatgagaga aggaattgta tgttatccta aaatcatgat tcctctgaca
     1321 agtcatgtga atgaactgaa gctgcagcag acaactctgg aggatgaagc aaagcaggtg
     1381 atgcaggaaa tgggtatgag tcttcagtac aagttcggaa caatgattga aattccgaga
     1441 gcagcaatga ctgcagatga actcgctgaa tatgcatcat tcttctcatt cggaacaaat
     1501 gatctgactc agacaacata cgggatatcc agagacgatg cagaaaaagg cttcctgatt
     1561 gaatatctgg aaaaaggaat tctgaaggat aatccattcg ccactatcga cagatacggc
     1621 gtaggaaaaa taatgaaaat tgctgtagat ctgggaagaa aagcaagatc cgatctggaa
     1681 atcggaatct gcggagaaca tggaggtgag cctctttcag ttcatttctg ccatgagatc
     1741 ggactgaatt acgtgagctg ttccccattc agagttccta tcgccagact tgcagcagca
     1801 cagatcgtac tgaaagaaaa gggtgtag
//
LOCUS       DAOQGJ010000842         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45457_length_1827_cov_2.669865, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000842 DAOQGJ010000000
VERSION     DAOQGJ010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45457_length_1827_cov_2.669865"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1538
                     /gene="rho"
                     /locus_tag="PK683_12795"
                     /pseudo
     CDS             237..1538
                     /gene="rho"
                     /locus_tag="PK683_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
     gene            1551..1751
                     /gene="rpmE"
                     /locus_tag="PK683_12800"
     CDS             1551..1751
                     /gene="rpmE"
                     /locus_tag="PK683_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNH09372.1"
                     /translation="MKDGIHPKYLDAKIRCACGNVIETKSTRGDISVEICSACHPFYT
                     DKAKAVNTAGRIDKFKKKYKMK"
BASE COUNT          617 a          328 c          409 g          473 t
ORIGIN      
        1 atcctcaata ttccaaagga ctttatgctc gcgattcatt ccgggcagaa tctaatactc
       61 tgaattgaat ttgtcttaag ttcaaatcag gctaaaaatc caacaaaaaa tcaaaaacta
      121 tggcagtcag aaaaagagaa gaaacacagc aggttcaaga aggaacagct tcaagaaatg
      181 aagcagagtc ttccgaatat tccagtttgg acaacctcgg agagtatatg ggcggtaacg
      241 gtcgaaatct ccgcaagaaa agaaaatatg aaggtccgca tccggaacct attgatctga
      301 caaagatcaa acagaagtcc attacggaac ttgtagacac agctaaaagt atgggtgtgg
      361 agaatacaag cggtctgaaa aagcagaatc tgatttttgc gattctgcag gctcagacag
      421 aaaaagaagg tcaggtgtat gcttccggag tgatggaaaa actgcctgac ggatatggtt
      481 ttttacgttc cccagactac aattatgtcc caggtcctga tgatatttat gtttccccct
      541 ctcagatcaa actttttgga cttcgaacag gagatacggt agaaggacaa atcagacctc
      601 ctaaggaagc agaaaggttt tatgcaatgc tcagagtgga gtctatcaat ggagtatcac
      661 ctgatattgc atctaaaaga gctctctttg ataacctgac tcctttatat cccaatcaaa
      721 gaatcaatat ggagtatgat ccttctcatt tggatactag gattctggat atgatggctc
      781 cgatcgggaa aggacagaga ggactgattg ttgcccctcc taggacagga aaaacagtgc
      841 ttatgcagag tatcgcaaat gcaattacta aaaatgatcc taaaacaata ctgattgtgc
      901 ttctgattga tgaaagacct gaggaagtga ctgatatggc cagaaatgtg agaggggaag
      961 ttgtcagttc cacttttgat gaacctgcac agagacacgt tcaggtggca gagatggtta
     1021 ttgaaaaggc taaaagactt gtggaacatg gaagagatgt agtcattctt cttgactcta
     1081 tcacaagact tgccagagcc tataaccagg taatcccgac ttccgggaaa attctttccg
     1141 gcggtgtgga ttcaaatgct cttcataaac cgaaaagatt tttcggggca gcaaggaatc
     1201 tcgaagaggg cggttccctt acaatcatag ctactgctct cattgaaact ggttccaaaa
     1261 tggatgaagt gatttttgag gaattcaagg gtacaggaaa tatggaagtt catctggaca
     1321 gaaaactttc tgacaaaaga atttttcctg ctattgatat caataaatcc ggaacaagaa
     1381 aggaagaact tctgctccgt tcggatgttc ttcaaaaagt atttgtgctc aggaaggtgc
     1441 tctctccaat gagtattaca gagagtatgg aattattatt ggataaaatg cgcggttcta
     1501 aaagcaatga agcttttctt gccagcatga atgcataaaa ggagattaca atgaaagatg
     1561 gaattcaccc aaaatatctg gatgcaaaga tcagatgcgc atgtggaaat gtgattgaaa
     1621 ctaagtccac aagaggcgat atatctgtag agatctgttc agcatgtcac ccgttttaca
     1681 ctgacaaagc aaaagcagtg aatacagctg gaagaattga caagttcaag aaaaaataca
     1741 aaatgaaata agaactttcg ttaatgagta tttctggaaa aagtgcaggc agagcagaaa
     1801 ggctctgctt tttttttata tatcagt
//
LOCUS       DAOQGJ010000843         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45538_length_1825_cov_2.998870, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000843 DAOQGJ010000000
VERSION     DAOQGJ010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45538_length_1825_cov_2.998870"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..765)
                     /locus_tag="PK683_12805"
                     /pseudo
     CDS             complement(1..765)
                     /locus_tag="PK683_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
     gene            complement(937..>1825)
                     /locus_tag="PK683_12810"
     CDS             complement(937..>1825)
                     /locus_tag="PK683_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH09373.1"
                     /translation="FGKETLTALENADFTVSENEVIGLAGESGSGKSTLGKAMLQLVP
                     ASGQVIYSGKNLIQLKRKELLPLRKDLQIIFQDPYSSMNPRFTVFEILTEGLKIHSSV
                     SPSEAKEKAESAMEKVGLKKEHLERYPHEFSGGQRQRICIARTLILNPKFIVCDEITS
                     ALDVSNQAQIINLLTGLKRETGISLLFISHDLGVLGHISDRIAVMYLGRIVELGSREE
                     IRKNPAHPYTRALFSSIFSLGGHRKSAPVTGEIPSVLNKPKGCYFHTRCPEAKDICRN
                     ERPAGKEVSKGHFSECHFS"
BASE COUNT          432 a          434 c          388 g          571 t
ORIGIN      
        1 gaggatgttt ttcagtacaa gaggagaaag tgtcgcagga taatccggat caatcttcga
       61 aaatactata tgtgcactca ttacagatct cgcaccttct tcgacggcct tcttgaaagg
      121 cagaagttca aactgcatca gttcttcttc tgtcttgtct atcaccggaa gaccgatatg
      181 actgtccaca ttcgtgtccc cgtgtcctgg aaaatgtttg atgactggaa gtgctcctgc
      241 agacctcgca cccttttcat atgcaattcc catcctgatc acagtttcag gcgtgacccc
      301 gaaagaacgg gtgttgatta caggattttc cggattgttg ttgatgtcta gatcaggagc
      361 aaacactgta ttgatcccca gttttttcag ctggtacgca gttgcaaatc ctatcttgta
      421 tgcatagtcc gaatttgagg tctggcccac tgccatcgcg cttggaaact gagtcacccc
      481 gctttccact cgcacgactc ttccgccttc ctggtcagtg gatatcagaa gaggaggaag
      541 tttcagttct ttcatttctg cctgaagatc ttttgtaagt gttcttattt cttcctttgt
      601 tccgagattc attccgaaaa ggatgactcc ccccggcctg atcttcctga tttcctttaa
      661 agcgtcctca tccgcttcct ttcctgcaat tgccacatga atgatctgtc ctgccttcac
      721 ttcatcgctc attcgagaca caatttccgc tgcaagtttt tccatttttg ccctgggagg
      781 aacagggggc agttcctgag aacagagttt tttcgggaaa taccagacag aaacaatgac
      841 cgcaactgtg atcagaaaac ctcgtgcgaa ctgaaacatg ggagttacct cttttttgcc
      901 tacaggctgt attggattat aatgtttttt taatgcttat gaaaaatggc attctgaaaa
      961 atgtcccttg gatacttctt ttcctgccgg tctttcattt ctgcagatgt cttttgcttc
     1021 cgggcatctg gtgtgaaaat agcacccctt cggtttgttc agaacactgg gaatctcgcc
     1081 ggtgactggt gcggattttc tgtgcccgcc taatgaaaat atactggaaa ataatgctct
     1141 tgtataggga tgtgcaggat ttttcctgat ctcttccctg ctgcccagtt ccacaattct
     1201 tcccaggtac atgactgcaa ttctgtccga gatatggccc agcacaccca gatcatgact
     1261 gatgaaaaga agagagatgc cggtttccct tttcagtcct gtcagaagat tgataatctg
     1321 tgcctggttg gatacatcca gtgctgatgt aatctcatcg catacaataa acttaggatt
     1381 cagaataagt gtccttgcaa tgcagatcct ctgtctctgt cctcccgaaa attcatgagg
     1441 atacctttcg agatgttctt ttttcagtcc cactttttcc attgcagatt ctgctttttc
     1501 ttttgcctct gaaggggata cggaactgtg gattttcagt ccctcagtca gaatttcaaa
     1561 aacggtgaat ctgggattca ttgaagagta ggggtcctga aaaataatct gcagatcctt
     1621 tcggagagga agcagttctt ttcttttcag ctgaatcaga ttttttccgg aataaatgac
     1681 ttgacctgag gctggaacga gctgaagcat tgccttgccg agagtcgatt tccctgaacc
     1741 ggattctcct gcaagtccga tgacctcatt ttcagataca gtaaaatctg cattttccag
     1801 tgcagtcagg gtttcctttc cgaac
//
LOCUS       DAOQGJ010000844         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45568_length_1824_cov_3.457321, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000844 DAOQGJ010000000
VERSION     DAOQGJ010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45568_length_1824_cov_3.457321"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PK683_12815"
     CDS             complement(<1..1255)
                     /locus_tag="PK683_12815"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNH09374.1"
                     /translation="MNQDNKTAYNISVSEKESNAETDLRPVSVGAYRFLDLSAFKRGI
                     SHGLGFIFTLGASALLAVAVTGTVNTFVNGSVMEASQLNTNFSTLKTAIEGITSSQWT
                     SNGSSIYYNAGNVGIGTVSPTSTLDVQNKLVFPLPGTSGRPILKLASTASVGGGFQFN
                     SNSTDYFYQYYDNNGIVFNAGTWTESPSSAIMIIKPSGNVGIGTTTPGARFTLNQAAE
                     TNTEGIRIVDGSVTWNIFSNAGGRLNFTSTGGANINFRDDGVVLIGTSTLDGANLFTV
                     NSANVSKTGGGSWNSISDIRLKHSVQEYSGGLNLIEKIHPILFHYKDDKSISMKDEGQ
                     HIGISAQEIQKIIPEAVMQGRGGYLQFKQDYVFWTMLNAVKELKSLHDKETSSLSERL
                     DTEKKKNDKLAEELAAVKTHDRASQQ"
     gene            complement(1266..>1824)
                     /locus_tag="PK683_12820"
     CDS             complement(1266..>1824)
                     /locus_tag="PK683_12820"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNH09375.1"
                     /translation="GDQTSTRDSKQDIHHFNSNHILLEKIAKVPLCTFIYKNEAKGYG
                     EKAKRKLGFIADEVDPLFMDEQGRAIDQVSVNGILIGALQELKSLYDNESLKSKSQEK
                     SISDLLLQLKQATERLDTEKKKNDKLAEELAAVKTHDRVSQQNLQRIIEKQEKLLAET
                     DRRLRALETFQTAAANANAGKERKQ"
BASE COUNT          440 a          404 c          356 g          624 t
ORIGIN      
        1 tctgttgaga cgcacggtcg tgtgtcttta cggcggcgag ttcctctgca agtttatcat
       61 ttttcttttt ctctgtatcc agtctttcgg acagtgaaga ggtttcttta tcgtggaggg
      121 atttgagttc cttgactgcg ttgagcattg tccaaaatac atagtcctgc ttaaattgga
      181 ggtatcctcc tcttccctgc attactgctt caggaataat tttttggatt tcctgagcag
      241 agattcctat atgctgcccc tcgtccttca ttgaaatact tttgtcatct ttataatgaa
      301 aaagaatagg atgaattttt tcaatcaggt ttaatccgcc tgaatactcc tgtacactgt
      361 gtttcagtcg aatatctgaa attgaattcc aggaaccgcc ccctgtcttg gaaacatttg
      421 cactatttac cgtaaaaaga ttcgctccat caagggttga agttccgatt aaaacgactc
      481 catcatctcg aaaattaatg tttgctccgc ctgtcgatgt aaaattcagt cttcctcctg
      541 cgttggaaaa aatattccaa gtaacactgc catcaacaat tcgaatgcct tcagtatttg
      601 tttctgcggc ttgattcagt gtaaatcttg caccaggcgt cgttgttccg atcccgacgt
      661 taccgcttgg tttgattatc attattgctg atgatggact ttctgtccaa gtgcctgcat
      721 taaaaacaat tccattgttg tcataatact gatagaaata gtcagtggaa tttgaattaa
      781 attgaaaacc acctccgaca gatgctgttg aggctaattt aagaattggc cttccggatg
      841 taccgggtaa agggaatact aatttatttt gcacatccaa agtgcttgtc ggactcactg
      901 tcccaatccc cacattccct gcattataat atatgctcga accattgcta gtccactgcg
      961 atgatgtgat cccttcaatt gcagtcttga gagtgctgaa atttgtattc aactgagatg
     1021 cttccattac agaaccattg acgaaagtat tgactgttcc agtgactgcc actgcaagaa
     1081 gagcagatgc accgagagtg aagatgaatc ccagtccgtg agatattccg cgtttgaagg
     1141 cggaaaggtc gaggaatctg taagctccca cagagacagg tcttagatct gtttctgcat
     1201 ttgattcttt ctcagaaaca gatatattat atgctgtttt attgtcttgg ttcatttgtt
     1261 tctccttatt gtttcctttc cttccccgca ttcgcgttcg ctgctgctgt ctgaaaagtc
     1321 tccagtgccc tgagccttct gtcagtctct gcaagcagtt tttcctgctt ctctatgatg
     1381 cgctgaagat tctgttgaga cacacggtcg tgcgtcttta cggcggccag ttcctctgcc
     1441 agtttatcat ttttcttttt ctctgtatcc agtctttcag tcgcttgctt caattgaagt
     1501 aacaggtctg atatggattt ttcctgagac ttgcttttca gtgattcatt atcatataat
     1561 gatttcagtt cctgaagtgc cccgattaaa atcccattga cactgacttg gtcaatcgca
     1621 cggccttgct catccatgaa aagcgggtct acctcatctg cgataaaacc aagttttcgt
     1681 tttgcttttt ccccatatcc ttttgcctca ttcttataga taaatgtgca taatggaacc
     1741 ttggctattt tttctaacag tatatgattg gaattgaaat gatgtatatc ctgcttggaa
     1801 tcccttgttg aggtttgatc acca
//
LOCUS       DAOQGJ010000845         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45612_length_1823_cov_2.947964, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000845 DAOQGJ010000000
VERSION     DAOQGJ010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45612_length_1823_cov_2.947964"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PK683_12825"
     CDS             complement(<1..272)
                     /locus_tag="PK683_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09376.1"
                     /translation="MKKMEKLYLISLAAAMLLSFCGKENRNDEDFEKAVRRFKNEKSD
                     RIDRPRKSVLPFIAGKSMDPVWDINHPEIITVPEFAFKNQKGSTVT"
     gene            complement(241..738)
                     /locus_tag="PK683_12830"
     CDS             complement(241..738)
                     /locus_tag="PK683_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09377.1"
                     /translation="MLPANDSRLSEKGGLKYLNGNPYTGEIVTFFDGGLPESSVEYRD
                     GKKDGTEKIWYKNGKLLAERKYSDGKREGIHKGWHTDGEIRFYFSFKDDRFEGENWTW
                     YPNGQTETYSKFENGIQLGYKHWRSDGKIYMNYVMDGFERIGMYGSRLCRKAENEKDG
                     KTVSD"
     gene            complement(985..>1823)
                     /locus_tag="PK683_12835"
     CDS             complement(985..>1823)
                     /locus_tag="PK683_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09378.1"
                     /translation="FKSLQEALNESRDGDTILIPAGTYTDGTYTISKKLTLKGTGGFA
                     HFTAPKLIPNKKAVFIVQSDTVMENIEISGARVPDKNGAGIRYEKGNLTLIKCYIHDN
                     EMGVLSGVIPSGSAHIKDSEFSHNGLDGYSDSKYWGHGLYIGDIKSLKVENSRFHGTI
                     IGHHIKSRARSTEITGSTLDDGNGDASYNIDIPNGGTAVIRENMIIQGDKSPNRTMIN
                     YGGESEPYPSSSLLIEKNTFTNRRKQGSTGIYNRTKISASVKGNKFENVEKPLEGPGK
                     IQ"
BASE COUNT          426 a          407 c          347 g          643 t
ORIGIN      
        1 tctgtgacag tactgccttt ctgattcttg aatgcaaact ccggaactgt aatgatttcc
       61 ggatgattta tatcccaaac tggatccatt gattttcctg caatgaaggg aagaacggat
      121 tttcttggtc tgtctattct gtcggatttt tcatttttaa atcttcggac tgccttttca
      181 aaatcctcat catttctgtt ttcttttccg cagaaggaga gcagcatcgc tgcagcaaga
      241 ctaatcagat acagtttttc catctttttc attttccgcc tttctgcaga gtctgcttcc
      301 gtacattcct attctttcga atccgtccat gacataattc atatatattt ttccgtcaga
      361 tctccagtgt ttatatccca gctgaattcc attttcgaat tttgaatatg tttctgtctg
      421 cccgttcgga taccaggtcc agttttcccc ttcaaatctg tcatccttga aggaaaagta
      481 aaatcggatt tctccgtccg tgtgccagcc cttgtgaatc ccttctcttt tcccgtcaga
      541 atactttctt tctgcaagaa gctttccgtt tttgtaccag atcttctctg ttccgtcttt
      601 ttttccgtcc ctgtattcga cagaggattc aggcagacct ccgtcaaaaa aagtgacaat
      661 ttctccagta taagggtttc cgttcagata tttcagaccg cccttttcag acagacggga
      721 gtcgtttgcg ggaagcattc tgtctgagca tcctgaaaaa atacataaga caggaattag
      781 caatgtaatt atattcaatt tttttgaaat atacccggtc atcagaataa gccttgctcg
      841 ccagagtata ctgtattttc agaaattcaa tatttttttt aatcagcacc tgaatgccgg
      901 acagaataca ccttcagcag taaactcagt tctgatattc tttttcgtta cggctgcgaa
      961 tctgcgctct gccgacagag ggtattattg aatttttcca ggaccttcga gaggcttttc
     1021 cacattctcg aatttgttgc cttttacgga ggcactgatt tttgtcctgt tgtaaattcc
     1081 tgtacttccc tgctttcttc tgtttgtgaa tgtatttttt tctatcagaa gggaactcga
     1141 cggatagggt tcagattcgc ctccgtaatt gatcatagtc ctgttcggag atttatctcc
     1201 ctgaatgatc atattttcac gaatgactgc agtgccgcca ttcggaatat caatattata
     1261 gctggcatcc ccatttccgt catcaagagt cgagcctgta atttcagtgg aacgtgctct
     1321 gcttttgata tgatgtccta tgatcgttcc gtgaaatctg ctgttttcta ctttcaggct
     1381 tttgatatcg ccgatataaa gaccgtgtcc ccagtacttg ctgtctgaat agccgtccag
     1441 tccgttatgt gaaaattcag agtctttgat atgagctgat ccggaaggga tgactccgct
     1501 cagaaccccc atttcattat cgtgtatata gcatttaatg agtgtgagat ttcctttttc
     1561 atatcggatt cctgcaccgt ttttatccgg tactcttgct ccggagattt ctatattttc
     1621 cattacagta tctgactgaa caataaaaac tgctttttta ttcggtataa gcttgggtgc
     1681 agtaaaatga gcaaaaccgc ctgtgccttt caaggtaagc tttttgctga ttgtataagt
     1741 tccgtcagtg taagttcccg cagggatgag aattgtgtct ccgtccctgg attcgttcag
     1801 agcttcctga agggatttga att
//
LOCUS       DAOQGJ010000846         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45651_length_1822_cov_3.694397, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000846 DAOQGJ010000000
VERSION     DAOQGJ010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45651_length_1822_cov_3.694397"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PK683_12840"
     CDS             complement(<1..253)
                     /locus_tag="PK683_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09379.1"
                     /translation="MLSISALSSAAGAPGYSAAANKGAVYIFYSSFYSSGSSGITAGN
                     FNSASSTVTGAGNSDRFGFISAAADLDLDGYSELLAGAKG"
     gene            complement(476..1558)
                     /locus_tag="PK683_12845"
     CDS             complement(476..1558)
                     /locus_tag="PK683_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09380.1"
                     /translation="MKFFYKTLGIFAGVNEFLSRKSINFVQLNWLLTAGLIFGLFSAV
                     STMNHNLTVKNEVVKSDPKDLQKNYQEKSLYVSLEGTLVKSLPIEYGTKDKKTDKFTV
                     TDLYFPLVDKDLKKGVFILYPKDTDRKDSPGLTSVKGKLVPSNSAVQKEIIAYNEDIT
                     KNAGNAGLEFYTDFILEQNVLPDQVSHTADGIIVGILFILSFGLLYTWVKRYNIVQTV
                     KKNPAVSGVSRKQLEAFEGTASDTALRNFIDGEFSGKLTFEKGGDRNFNFIPARFNFM
                     EDSSVLIASNIDASSTFMGFTTSDRNGIWTKIFTKADLSSWNYAVQYFGTKTKPAVLL
                     KFNNGPDAVLSFDSEKDQEEFGAKIS"
BASE COUNT          513 a          393 c          346 g          570 t
ORIGIN      
        1 agcctttcgc acccgccaga agttctgaat atccgtccaa atccagatct gctgcagcag
       61 aaataaaacc gaacctgtca ctgttccctg cacctgtaac tgtggaactt gcactgttaa
      121 aatttcctgc tgttattccg gaagaaccgg aagagtaaaa agaagaataa aaaatgtaga
      181 ccgctccttt gtttgccgca gctgaatatc cgggggctcc tgctgccgag gacagcgcag
      241 agatacttaa cattgtcttc agttttagtt ttctgcccag ggcacaaaag tgaaaattca
      301 atgcaatcaa aaacagcaca gggaaagatt tcgcctgttt cagaagccga aaaaacttca
      361 aagttcctgt atcttcataa agcatttttt cttcctttat ggattgcatt gctgcacagt
      421 ccaacgagaa ggactgtcag tttatagaca gttcaggaca aatgattttt caaagtcagc
      481 tgatttttgc tccaaactct tcctgatctt tttctgaatc aaatgagaga acagcatcag
      541 gcccgttatt gaacttcagg agaactgcag gtttggtttt tgttccgaaa tactgaactg
      601 cataattcca ggaactgaga tctgcctttg tgaagatttt tgtccatatt ccgtttctgt
      661 ccgatgtagt gaatcccata aatgtggaag atgcatctat attcgaagcg atcagcacag
      721 agctgtcctc cataaaattg aatcttgcag gaataaaatt gaaatttcgg tccccgcctt
      781 tttcaaatgt cagttttccg gagaactcac cgtcaatgaa attcctcagt gcagtatcgg
      841 atgcagttcc ttcaaaggcc tccagctgtt ttctggaaac tcctgacact gcaggatttt
      901 tctttaccgt ctgaacaata ttgtaccttt taacccatgt gtacagcagc ccgaaagaga
      961 gaatgaaaag tattcccaca atgattccat ctgcagtatg agagacctga tccgggagta
     1021 cattctgctc gaggataaag tctgtataaa actcgagtcc agcatttcct gcgttctttg
     1081 taatgtcttc attgtatgca ataatttctt tctgaactgc agaattggac ggaacaagtt
     1141 ttcctttcac tgaagtgagg ccgggggaat cctttctgtc tgtatctttc ggatagagaa
     1201 taaatactcc ttttttcaga tccttgtcca caagcggaaa atacagatct gtcactgtaa
     1261 acttgtctgt tttcttgtcc tttgttccgt attcgattgg aaggcttttt acaagtgttc
     1321 cttcaagtga aacatataga gatttttcct gatagttttt ttgaagatcc tttggatcgg
     1381 atttcactac ctcatttttt actgtcagat tatgattcat cgtactgaca gcggaaaaca
     1441 gtccgaagat aagccctgcc gtcaggagcc agttcagctg tacaaagttg atgcttttcc
     1501 tgctcaggaa ttcattgact cctgcaaaaa tccccagtgt cttgtaaaaa aatttcatag
     1561 cggtctctca aaaagaagtg tgagatttca ttttttattt tataagtttt atgcaactaa
     1621 aaatatttgt cgctgacttg tttacaagct cagtttttta cagatctcaa aataaacaga
     1681 atgagtcaga tcaacagcaa agcgaaatta ttgccgaaac ctccgcacct ccgggtgatt
     1741 cccatttttt atgcttttac gacccgtcag aaaaatctct aatcctgccc tgctttgaaa
     1801 ttctgaattt ttctcattga ca
//
LOCUS       DAOQGJ010000847         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45659_length_1822_cov_3.222977, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000847 DAOQGJ010000000
VERSION     DAOQGJ010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45659_length_1822_cov_3.222977"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PK683_12850"
     CDS             <1..490
                     /locus_tag="PK683_12850"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09381.1"
                     /translation="EFWRKLPSYSQISIFDGSWYKKMVYERVLGDLSKKDFYKKLERT
                     EKIERMLIKDRYFISKIFLHISSDFQKKKFKKAIESGKKWMVTEQDELEKENYKEFDK
                     TYGKILDAYSETPWKIFRAEDKVLARKMILESLIGDLENFLGFDSDKMLQTMKDKEGA
                     AA"
     gene            487..1227
                     /locus_tag="PK683_12855"
     CDS             487..1227
                     /locus_tag="PK683_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose-lipid carrier transferase"
                     /protein_id="HNH09382.1"
                     /translation="MRTIFRLSDVIQPTPIEKWVYKKEIDSLQERAKLISHYADKKKH
                     NIILVFEGWDAAGKGGAIRRLTSLFDPRLYSVISIAAPNSEEKNHHYLWRFWTQLPKK
                     GRIAIFDRSWYGRVLVERVEKFAKEEDWKRAYDEIVLFEEELSSDDCTVIKFWLHITS
                     EEQLERFNARKDDPLKRWKLTDEDWRNRDKWGLYEEACEEMILRTHTEESPWIVVPAN
                     EKYHARITVLKSFCEVMEKRLGMPDKFK"
     gene            1227..>1822
                     /locus_tag="PK683_12860"
     CDS             1227..>1822
                     /locus_tag="PK683_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09383.1"
                     /translation="MREEEYLSVRKKIRERELLDSNLDDAEKELLSLSEKYPSEGRIY
                     GILSEIYYWRGEYSLGDKLEIYEKGAAYGEKGVKLSPGSVEANFWLGVNYGFLGLEKG
                     MLQSYMLLDPIEKHFKKAVEIDETFFHGAPHRSFGWFFHQIPPWPISKGDNKKAIDHL
                     KKSLKFGPEFPLTHLYIGQVFLAVKDWKNARVHLEWTV"
BASE COUNT          602 a          327 c          453 g          440 t
ORIGIN      
        1 tgaattctgg agaaaacttc cgagttacag tcagatcagc atttttgacg gctcttggta
       61 caagaagatg gtgtatgaga gagttcttgg agatctttcc aaaaaggatt tttataagaa
      121 gctggaaaga acagagaaaa ttgaaagaat gctcattaag gacagatact ttatatccaa
      181 aatatttctg catatcagtt ctgattttca gaaaaagaaa ttcaaaaaag cgattgagtc
      241 aggaaaaaaa tggatggtga cggaacagga cgaactcgaa aaggaaaatt ataaagaatt
      301 tgataaaaca tacggtaaga tactggacgc ctattcggaa actccctgga agatattccg
      361 tgctgaggac aaggttctcg ccagaaagat gatcctggag agtctcatcg gagatctgga
      421 aaattttctc ggatttgatt cagataaaat gcttcagaca atgaaggaca aggaaggagc
      481 agccgcatga gaacaatatt cagattatca gatgtcatac agcccacacc gattgaaaaa
      541 tgggtgtaca aaaaggaaat cgacagtctt caggagagag caaagctcat ctctcattat
      601 gcggacaaga aaaagcacaa tatcattctt gtatttgaag gctgggatgc cgcagggaaa
      661 ggcggggcta tccggagact gacttctctt tttgacccca gactgtattc ggtcatttcc
      721 attgccgctc cgaactctga agaaaaaaat catcactatc tctggaggtt ctggactcag
      781 cttcccaaga aaggaaggat tgccattttt gacagatcct ggtacggaag ggttcttgtg
      841 gagagagtcg aaaaatttgc gaaggaagag gactggaaaa gagcctatga tgaaattgtt
      901 cttttcgaag aggaactcag ctcagacgac tgcactgtaa tcaaattctg gcttcatatt
      961 acaagcgaag agcagctcga aagattcaat gcaagaaaag acgatcctct caaaagatgg
     1021 aagctcactg acgaggactg gagaaacagg gacaaatggg gactctatga ggaagcgtgt
     1081 gaagagatga ttctccgcac tcatacagaa gaaagcccct ggattgtggt tcctgcaaat
     1141 gaaaagtatc atgcaaggat cactgtgctg aaatcattct gtgaagtaat ggagaaaaga
     1201 ctcgggatgc ccgacaagtt caagtgatga gagaagaaga ataccttagt gtcagaaaaa
     1261 aaatcaggga aagagagctt ctggattcca atctggacga tgcggaaaaa gaacttcttt
     1321 ctctttcgga aaaatatcct tcggagggaa ggatatacgg tatcctttct gagatatatt
     1381 actggagggg agaatactct ctcggtgaca agctggagat ttatgagaag ggagccgcat
     1441 acggggaaaa gggagtcaaa ctgagtcccg gttcagtgga ggcaaatttc tggctcggag
     1501 tgaactacgg atttctcggt ctggaaaagg gtatgcttca gagttatatg ctccttgatc
     1561 cgattgaaaa acatttcaaa aaggcagtgg agattgacga gacattcttt cacggggctc
     1621 ctcaccgttc cttcggatgg ttttttcacc agattcctcc ctggccgatc tcgaaagggg
     1681 acaataaaaa agcaatagat cacctgaaaa aatcattaaa attcggaccg gagtttcctc
     1741 tgacgcatct ttatatcggt caggtgtttc tggctgtgaa ggactggaaa aatgcaagag
     1801 tccatctgga atggactgta aa
//
LOCUS       DAOQGJ010000848         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45703_length_1821_cov_3.324462, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000848 DAOQGJ010000000
VERSION     DAOQGJ010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45703_length_1821_cov_3.324462"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PK683_12865"
     CDS             complement(<1..981)
                     /locus_tag="PK683_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH09384.1"
                     /translation="MNKVLVTGGCGFLGSHVCEYFRKEGWEVISYDNMTKYELKRTGY
                     GTDATRDFNWNFLKGIGVEMVKGDIRNLEHLLDRTHGCSYIVHTAAQPAMTVSWEDPE
                     YDFTNNVVGTFNVMEAARQRKIPVVNTSSIHVYGNAINDTLKEGETAYTRDPVEIPET
                     QPVMVGKLSPLHASKMSAEHYVRTYHDMYDVKSATFRFTGIYGERQFGGEDHGWVANF
                     AIRSVFGLPIRIFGTGKQTRDIIHAEDGAASYLNFFKNPIHGVYNIGGSKEHKISLLE
                     CIAMIGEILGRKQEVIFEAERPGDMRYFICDITAAKKFGFNPKIRPRQGVE"
     gene            complement(984..>1821)
                     /locus_tag="PK683_12870"
     CDS             complement(984..>1821)
                     /locus_tag="PK683_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH09385.1"
                     /translation="KESGKVYMTPVHCTYYNINSMQDYHQAVYEIRHDLFDNYKVSLI
                     IPTKNNERSLPDVITDFKDKVHEIVIVDGNSTDKTASIAKEFKTQLEMYYSPDGNENE
                     GVKIREGIEKATGDIIVVVSADGAFRSKDLLKLLEYVKDCDMVIGTRTTRQMIEQGSN
                     LKAFSRLVNLILGKIVEVFWWGQEPRFTDVDCKFFAVWKDSYSKIKSRLTVKNESYPL
                     EMMIEIVRSHMRCIEVPISYFNPVEKEHYRKRTVFKKGLKALRLALSKKKRDYFPDRL
                     ED"
BASE COUNT          457 a          442 c          346 g          576 t
ORIGIN      
        1 ttcgacgccc tgtcttggac ggattttagg attgaagccg aatttttttg ctgcagtgat
       61 gtcgcagatg aagtatctca tatcgcctgg acgttctgct tcaaagatga cttcctgctt
      121 tcttccgagg atctcaccga tcatagcaat gcattccaga agggaaattt tatgctcctt
      181 ggagcctccg atattgtaca cgccgtgaat cggattcttg aagaagttca gataacttgc
      241 cgcaccgtct tctgcatgga tgatgtctct agtctgtttt cctgttccga aaatccggat
      301 gggaaggccg aatacagagc ggattgcaaa atttgccacc caaccgtgat cctctcctcc
      361 gaattgtctc tccccgtaaa tcccagtaaa acggaatgtg gcacttttca catcatacat
      421 atcatgatag gttctcacat aatgctctgc actcatcttg gatgcatgaa gaggagaaag
      481 ttttcccacc attaccggct gagtttcagg aatttccaca ggatctcttg tataggcagt
      541 ctcgccttct ttcagagtat cattgattgc gtttccatac acatggatgg agcttgtatt
      601 gactacagga atctttctct gtctggccgc ttccataaca ttgaatgttc ccaccacgtt
      661 gtttgtgaaa tcatattccg gatcttccca ggaaactgtc attgcaggct gagctgcagt
      721 gtgaacgata tagctgcatc cgtgcgtgcg gtcgagaaga tgctccaggt ttctgatatc
      781 acccttgacc atctccactc cgatgccttt caggaaattc cagttgaaat ctctcgttgc
      841 atccgttccg taaccggttc ttttcagttc atatttggtc atattatcat aactgataac
      901 ttcccatcct tcttttctga aatattcaca tacatgtgat cccaaaaatc cgcatccgcc
      961 agtcacgagc accttattca ttcttaatcc tccagtctat cgggaaagta gtctcttttt
     1021 ttctttgata atgcaagtct caatgccttg agaccttttt taaacacagt tctttttctg
     1081 taatgttcct tttctaccgg attgaaatag gaaataggca cctcaatgca tctcatatgc
     1141 gaacgtacta tttctatcat catttccaga ggataggatt catttttgac agtcagtctg
     1201 gacttgattt tcgaatagga atccttccaa acggcaaaaa acttgcagtc cacatccgta
     1261 aatcggggtt cctgtcccca ccagaacact tccacaattt ttccaaggat aagattgaca
     1321 agcctggaaa aggctttcag atttgaaccc tgctctatca tctgtcttgt ggtgcgggtt
     1381 ccaatcacca tatcgcagtc ttttacatat tccaggagct taagaagatc cttcgatcgg
     1441 aacgcaccgt ctgccgaaac gaccactatg atgtcgccgg tcgctttttc aatcccctct
     1501 ctgatcttta ctccctcatt ctcatttccg tccggagaat agtacatctc cagctgagtc
     1561 ttgaattcct tggcaatcga agcagtcttg tcagtggaat ttccgtccac aattacaatc
     1621 tcatgcacct tatccttgaa atctgtaatc acatcaggaa gagaacgttc attgttcttt
     1681 gtcggaataa tcagtgatac tttataatta tcaaaaagat catgtctgat ttcgtaaact
     1741 gcctgatgat aatcctgcat ggaattgatg ttgtagtaag tgcagtgaac aggagtcata
     1801 tatactttgc cagactcttt t
//
LOCUS       DAOQGJ010000849         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45739_length_1820_cov_3.486686, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000849 DAOQGJ010000000
VERSION     DAOQGJ010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45739_length_1820_cov_3.486686"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(549..>1820)
                     /locus_tag="PK683_12875"
     CDS             complement(549..>1820)
                     /locus_tag="PK683_12875"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNH09386.1"
                     /translation="LSLSSTAIIVKLLYDKFEVEGLAGKATIGILIFQDIWAILFLAV
                     QPNLKTPGFMVILGAVLKAVFLVIWGILVSRYVLKRVFQSISSPEMTFTVAIGWCIAN
                     AGLGHLIGLSSEMGALIAGLVLSTHPFSVDISSRVLPLRDFFLTLFFISLGMKIPMPE
                     MSILKSSLIIIFFIPFIRILIIFPTMFFFGNSRRTSILTALNLSQISEFSLVIASIGF
                     ADGHISKDVFAVLLYTLAVSSVTGSYIIKFNHEIVGLVSRVFKKIKPYESSPPETEAP
                     RKVFILGYHRGIRGLIHSLYQTNPELLREISVIDFNAETKAELEIFGIRTFIGDISNS
                     DNLRHAGIQHAKIIISTIPDAILKGTDNSRLVKLLRTLSPESFIITHGDYVQQVKGLL
                     EAGADDVILPYIIAGEHLADKISSIARKLEN"
BASE COUNT          527 a          391 c          380 g          522 t
ORIGIN      
        1 agattcaggc ccttcagtgt aatttgaact ctgatcgatc tcattttggt gagtccaatc
       61 aaattgtgac tggagtatcc gtttttttcg aaaaactcat tcagataatt gtgtacagca
      121 cctttcatga ggttatcatg gaatacattg caaatcggtc aagaaagttt gtaaaagaat
      181 tttagagttt tcttaaattg atataagccg cgattcggaa gctgccggac agtcggaaaa
      241 aacttgacaa agaccgattc tgagcagata ctgcattatg aaaaatttca gacttctgtc
      301 agcatttctc atcttttgct tctctgttca ctgcggtaca gtttcaagtt ttgcctattc
      361 agaaagctat cgtcaccgct attttatata ttccgggttg cagaaagact ttgacttaat
      421 cggagacccg aaaatctttc ctgcaggacc gatcttcatt cttgatctgc ccttcagttt
      481 tgttctcgat acagcgcttc ttcccttcac tgttcttcat tttttcatca ggaaagattc
      541 cccctaattc agttttccag ctttctggct atagaggata tcttatccgc caagtgttct
      601 cctgcaatga tgtaaggaag gatcacatcg tctgcacctg cctcaagaag accttttacc
      661 tgctgcacat aatctccgtg cgttattatg aatgattcag gagaaagagt tctgagaagt
      721 ttcaccagtc ttgaattgtc agtgcctttc aggattgcat ccggtattgt agatataatg
      781 atttttgcat gctgaattcc tgcatgacgg agattgtcgg aattggaaat atccccgatg
      841 aatgttctga ttccgaatat ttccagctcg gcttttgtct cagcattgaa gtctatcacg
      901 gaaatctctc gaagaagttc agggttggtc tgatagagcg aatgaataag ccccctgatt
      961 cctctgtgat atccgagtat gaataccttt ctcggggctt ctgtttccgg aggtgaactt
     1021 tcatacggtt ttattttctt gaagactctg gatacaagtc ctacaatttc atgattgaat
     1081 tttatgatgt aggaaccggt gacggaactg actgcaagag tatacaggag cactgcaaac
     1141 acatccttgg aaatatgacc gtccgcaaaa ccgatgctgg cgattacaag agagaattca
     1201 ctgatctgcg aaagattcag cgcagtcagt atggaagtcc ttctgctgtt cccaaagaag
     1261 aacattgtcg gaaaaataat aagaattctt ataaatggaa taaaaaagat gattatcagt
     1321 gaacttttca gtatggacat ctcgggcata ggaatcttca tcccgagcga aataaagaaa
     1381 agagtcagaa aaaagtcccg gagagggagt actctggaac tgatatctac agaaaatgga
     1441 tgagtggaaa gcacaagtcc cgcaatcagc gcccccattt cagaggaaag accgatcaga
     1501 tgtcccagac cggcatttgc aatgcaccat ccgatggcaa cagtgaatgt catttcagga
     1561 gaactgatcg actgaaacac cctctttaga acgtaccggc tgacaagaat tccccagatg
     1621 acaagaaaga cagccttcag taccgctccc agaatcacca taaatccggg agttttcaga
     1681 ttcggctgga ctgcaagaaa cagaatcgcc catatatcct gaaaaatcaa tattcctatg
     1741 gtggcctttc ccgcgagtcc ttccacttca aacttgtcgt acagaagctt gacaataatc
     1801 gcagtactgc tgagggaaag
//
LOCUS       DAOQGJ010000850         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45778_length_1819_cov_3.327664, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000850 DAOQGJ010000000
VERSION     DAOQGJ010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45778_length_1819_cov_3.327664"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1478)
                     /locus_tag="PK683_12880"
     CDS             complement(165..1478)
                     /locus_tag="PK683_12880"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09387.1"
                     /translation="MNLRRELSREFAVYFIFHLREELKKPSEVTDYITLEGESFRTQT
                     ENMIDDLKKVFPNLQERIPLFNFFKQWWNRQRVNVNSSLIYENSTFKAYNQMFFFRIM
                     DIRFHENSLRKKFTENMKNQTRMIREKGRLVTFEMLFQGELVLYPILELMLKRSRLPF
                     REGRKILYEQSSFIQKAAYEVIDSELRDSLARTDSILKAILPEKIAEELKQKKKVQPK
                     LHNSISIMFCDLAGFTEISSRLAPEDLLRELNECFGHFDKITSMLGLEKIKTIGDSYM
                     AAAGLNDVKVLHSVNSILAASRIHEFLRKYAKSQNRKGLPAWQVRIGIHTGPAVAGIL
                     GENRFNYDIWGDSVNVAQRMESYGEAGRINVSRETMESASDFFIFEEGRTVNVKGKGM
                     LEMFFVKSIKPELSLNGSGKTPSAKFKRLYESVMNPEAGLNSSQS"
     gene            complement(1487..>1819)
                     /locus_tag="PK683_12885"
     CDS             complement(1487..>1819)
                     /locus_tag="PK683_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH09388.1"
                     /translation="VLGIRGLNLSGGQRQRLVIARALLRNPEILILDEATSALDSKSE
                     KLVSAALDRLFRNRTTFIIAHRLSTIRKIRRILVIDGGKIAEEGDHESLLSKGGLYSR
                     LYENQFAG"
BASE COUNT          506 a          415 c          360 g          538 t
ORIGIN      
        1 tattttttaa ttttcaatac tttctgtcaa acctacagga agatcctgta tggaaaaagt
       61 aattacaata cgtcggaaaa gagtgaacag atatgcatca ggccgacatt aaaatcagtc
      121 tatcggcaaa tgtcaaatcg gagggcttgc gggaccagag cagtctagga ttgagaactg
      181 tttaaacctg cctcgggatt cataacggat tcataaagtc gcttgaattt agctgaaggc
      241 gttttaccgg aaccgtttaa ggacagttcc ggcttaatac tttttacaaa aaacatttca
      301 agcattccct tgcccttgac atttactgtt ctcccctctt caaaaatgaa gaaatcggaa
      361 gctgattcca tagtttcgcg ggatacattg attctgcctg cttcaccgta agactccatt
      421 ctctgtgcaa cgttgacaga atctccccag atatcataat tgaagcggtt ttcccccagt
      481 atgcctgcaa cagccggacc ggtatgtatt cctatcctca cctgccaggc aggaagacct
      541 ttcctgttct gactctttgc gtattttcgt aaaaattcgt gaatacggga agcggcaaga
      601 atgctgttca cagaatgaag gacctttaca tcattcagac ctgcggcggc catataggaa
      661 tcccctatcg tctttatctt ttcgagaccg agcatagaag ttattttgtc aaaatgcccg
      721 aagcattcat tcagctcccg aagaagatct tccggtgcaa gccgcgaaga tatttcagta
      781 aaaccggcaa gatcacagaa cattattgag attgaattgt ggagtttagg ctgcactttt
      841 ttcttctgct tcagttcttc tgcaattttc tccggaagaa tagccttcag aatagaatca
      901 gtccgtgcaa gagagtccct cagctctgaa tcaatcacct cataggctgc tttctgaatg
      961 aaactgctct gttcgtaaag aatctttctt ccttctctga atgggagccg acttcttttg
     1021 agcatcagct caagaatggg atacagtacc agttccccct gaaaaagcat ctcaaaggtg
     1081 acaagccttc ccttctcgcg gatcattcta gtctgatttt tcatgttttc agtaaacttt
     1141 tttctaagcg aattttcatg aaaacgtata tccataatcc tgaaaaaaaa catctgattg
     1201 tacgctttga atgtggagtt ttcatatatc agagatgagt ttacatttac tctctgccga
     1261 ttccaccact gtttgaaaaa attaaataga ggtatccgtt cctgaaggtt gggaaaaact
     1321 tttttcagat catcaatcat attctctgtc tgagtacgga agctttctcc ttccaaagtg
     1381 atataatctg tcacttcaga aggttttttg agctcttcgc ggagatgaaa tataaaatat
     1441 acagcaaatt cccggctcag ttccctgcgc agattcattt cagcctttat ccggcaaact
     1501 gattttcata aagacgggag tacagaccgc ccttggacag aagcgactca tgatcgccct
     1561 cttcggcaat tttaccgccg tcgatcacaa gtatacgtct gattttccgt atagtggaaa
     1621 gtctgtgagc aatgataaaa gttgtcctgt tgcgaaaaag cctgtctaac gctgcactta
     1681 caagtttttc agatttgctg tctaaagcac tcgtcgcctc atcaaggatg aggatttcag
     1741 ggtttctaag gagtgctcgg gcaatgacaa gtctctgcct ctgacccccc gagagattca
     1801 agccccgaat tccaagaac
//
LOCUS       DAOQGJ010000851         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45836_length_1818_cov_2.304594, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000851 DAOQGJ010000000
VERSION     DAOQGJ010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45836_length_1818_cov_2.304594"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..399)
                     /gene="msrB"
                     /locus_tag="PK683_12890"
     CDS             complement(1..399)
                     /gene="msrB"
                     /locus_tag="PK683_12890"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020780808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNH09389.1"
                     /translation="MEDKIVKTDEEWKKVLSEEAFYVLRKKGTERAFTGKYHAHKEKG
                     IYICAACGYELFSSDTKFDSGTGWPSFYEPVKEGNVGKEIDKGFFMTRTEVHCARCGG
                     HLGHVFSDGPKPTGLRYCINSVSLDFKKKD"
     gene            668..880
                     /locus_tag="PK683_12895"
     CDS             668..880
                     /locus_tag="PK683_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09390.1"
                     /translation="MNRKENAAKVSFSEYSEEVMNMRTLLENLAEQLRNQPKVRKRKL
                     YKKVRISRKKILQGLPTLPFTEAGES"
     gene            1207..1746
                     /locus_tag="PK683_12900"
     CDS             1207..1746
                     /locus_tag="PK683_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09391.1"
                     /translation="MAEVKGKFITLSCTLLETKPKAKQEALQKVKEMTGKEFDELDPE
                     GWYDTKVFQAVFSSIEANTSVLLAKAAIKLIGQKVYPTIEKTAGLPLHLKTPLDFIKF
                     EAEGFLQNHRGADVQPRKIIAAVDGNVVIQANSPGYSPLLIEGVFLGILEMCGIRTGK
                     VDMKEEDGNCVYYITWQPI"
BASE COUNT          550 a          370 c          364 g          534 t
ORIGIN      
        1 tcagtccttc tttttaaaat ccagtgagac agaattgatg cagtaccgga gtccggtagg
       61 tttcggtccg tcgctgaata catgccccaa atgacctccg catctggcac agtgcacctc
      121 ggttctggtc ataaaaaaac ctttgtcgat ttccttgcct acgtttcctt cttttacagg
      181 ctcataaaaa ctcggccatc cggttcctga atcaaatttt gtatcagagg aaaaaagttc
      241 atatccgcat gccgcgcata tgtagattcc tttttcctta tgagcgtggt actttcctgt
      301 aaatgccctt tcagtccctt ttttgcggag aacataaaat gcctcctcac tcagaacttt
      361 tttccattcc tcgtcagttt tgactatctt gtcttccatt ctgcggacct catttttctc
      421 tttttttact gactgttctt ttgcaaaagc agaattcagc agtagaacag cacctgcaat
      481 aaaaatcttc aacatattgt ttcctttctg gttaaatttg aaacttctgc actgaactta
      541 gacgaaatcc ctctgttcaa tgcgcatttc acggtatgat ttctctgaaa aaataaaaat
      601 gttacccttc ggatcttatt ttgtccggtt ttcacgtatt atatatacag aagagcagga
      661 ggacgcgatg aacagaaaag agaatgcagc aaaggtttcc ttttcggaat attccgaaga
      721 agtgatgaat atgagaactc tgcttgaaaa cctggcagag cagctcagaa atcagccgaa
      781 ggtccgaaaa agaaagctct acaaaaaggt caggatttcg agaaaaaaaa tactgcaggg
      841 tcttccgaca cttcctttta cggaagccgg agagagctga gcctgattca gtccggaaat
      901 ctttgcttcg tgcaaggctc cggatctctg aaactcggtc tgacgttggt catatcaccg
      961 gaaagaccga cgtgaaaaga gaactgcatt cagatcctgc agaatataat gtttaaaaaa
     1021 gattaaatca gatatgtgtc agtttttgaa aaaataacag tatttaaaac gaggccattt
     1081 ttctgccgta tctaaaaatg agaatcattc ttattattta ttgatttata atcctattcc
     1141 cgattttaag atgctcaaag acagactggt ctttacggca gtccgaaaaa aattacggag
     1201 aagattatgg cagaagttaa aggaaaattc attactcttt catgcacact tttggaaact
     1261 aagcctaagg caaagcagga ggctcttcag aaagtaaaag aaatgacagg aaaagagttc
     1321 gatgaacttg atcctgaagg ctggtatgac actaaagttt ttcaggctgt gttcagttca
     1381 attgaagcga atacatcagt tcttcttgca aaggcagcaa tcaagctcat tggtcagaaa
     1441 gtgtatccta caattgaaaa gacagcaggt ctgcctcttc atctgaagac accgctcgat
     1501 tttatcaagt ttgaagcgga aggatttctt cagaatcaca gaggagctga tgttcagccc
     1561 agaaagatca ttgctgctgt tgacggaaat gtagtgattc aggcaaattc acccggatat
     1621 tctcctcttc tgattgaagg tgtatttctc ggaattctgg aaatgtgcgg aatcagaacc
     1681 ggaaaagtgg atatgaagga agaagacggc aactgtgtat actacatcac ctggcagccg
     1741 atttaaacaa gctgaattcg tcgattgaac ggattcattt taaaaatttt ttcattaaat
     1801 cagcagtatt tctttccc
//
LOCUS       DAOQGJ010000852         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_45891_length_1817_cov_2.361521, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000852 DAOQGJ010000000
VERSION     DAOQGJ010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45891_length_1817_cov_2.361521"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1011)
                     /locus_tag="PK683_12905"
     CDS             complement(1..1011)
                     /locus_tag="PK683_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH09392.1"
                     /translation="MKKYFLKRFLLIFPTLLGITFIVFLISHLAPGGPLESELAKYRN
                     LGAEAGASQKKISQEEVDLLKKRLHLDKPVPVAYLYWLKQIASFDLGESRINYKKVST
                     LIADKLPISLTFGLTGFFLSYLICIPLGIYKALHNGGKADIYTSIIIFFTYSIPTFAF
                     AMLLLFLFASGQVFSFFPLGHEISDNYESLGPFEQILDRAHHMFLPVVCYSIHSFAVL
                     TLLMKNSLLEEISREYMRTAVAKGLSFKEAVKRHAFRNSLIPLATGFGSNLTLILAGS
                     LIIELVFNIDGIGLLSFTAVKQRDTDLMMGLLLVQSFLALFGRILSDYCYTLVDPRIR
                     FE"
     gene            complement(1011..>1817)
                     /locus_tag="PK683_12910"
     CDS             complement(1011..>1817)
                     /locus_tag="PK683_12910"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004451022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH09393.1"
                     /translation="DNIVFLGVLGTLVGSKIFYIFEIWVQIFVLPGNYMFPLTHWYGF
                     REYNDPSCQVCMESYGLWSSLFSGGGLVFYGGFLTGTLFIYYYLKSNKWDLGQYYDGM
                     APTMAMGYAIGRLGCFVSGDGCYGHATDVRIPLLVFKYDGAMPSGVPVWNTPVIESIV
                     SFVFFFYFFYWARNQNFKKWSLFFQYLFLHGLARLCIEFLRVNKAVFAFMKPPEIVNI
                     PDVGRNPEFLKSYYWHGFSQSQYISLALMLVSVIFIVRGKLWEKDSSAGA"
BASE COUNT          570 a          394 c          394 g          459 t
ORIGIN      
        1 tcattcgaat cggatcctcg ggtcaactaa tgtataacag tagtctgaaa ggattcttcc
       61 gaacagtgca aggaaactct gcacaagaag aagtcccatc atcagatcag tatctctctg
      121 cttgactgca gtaaaactta acagacctat cccgtctata ttgaatacaa gctcaatgat
      181 gagcgatcct gcaaggatca gtgtaagatt gcttccgaag cctgttgcga gagggataag
      241 agaattccgg aaagcatgtc ttttgactgc ttctttgaat gaaagtcctt ttgcgacagc
      301 agttctcata tattcccggc tgatctcttc gagaagggaa ttcttcataa gcagcgtcag
      361 aactgcaaac gaatgaatag aatagcacac cacaggcaga aacatatgat gggccctgtc
      421 caggatctgc tcaaaaggcc cgagggattc ataattgtcc gagatctcat ggcccagagg
      481 aaaaaaggag aatacctgtc ccgatgcaaa cagaaagaga agaagcatcg caaatgcaaa
      541 tgtaggtatg gaatatgtaa agaatattat aatgctggtg tatatatctg cctttccgcc
      601 gttgtgaagc gccttgtaga ttcctagagg gatgcagatc agataactca ggaaaaaacc
      661 ggtcagaccg aacgtgagcg aaataggaag tttgtctgca ataagtgtgc ttaccttttt
      721 gtagttgatt ctggattccc cgagatcaaa ggaagcaatc tgcttcagcc agtacagata
      781 tgcgaccgga accggcttgt ccagatgaag tctttttttc aaaagatcca cttcttcctg
      841 agagattttc ttctgacttg ctcctgcctc tgctcccaga ttcctgtact ttgcgagttc
      901 ggactcaaga ggccctcccg gtgcaagatg tgaaatcaga aatacaatga aagtgattcc
      961 gagaagtgtc ggaaaaatca gaagaaatct tttcaggaaa tattttttca ttatgcccct
     1021 gcagaactgt ccttttccca gagttttccg cgcacgatga aaatgacgga gacaagcatc
     1081 agtgcaagag agatgtactg agactgtgaa aatccgtgcc agtaatagga tttgaggaat
     1141 tcaggatttc ttcctacatc aggtatgttc acaatttcag gaggtttcat gaatgcgaat
     1201 actgccttgt tcactcggag aaactcgatg cagagtctgg caagtccatg caggaataaa
     1261 tactgaaaga aaagactcca ttttttgaaa ttttgatttc ttgcccaata gaagaaataa
     1321 aagaagaaaa cgaaagatac aatggactct atgacaggtg tgttccagac cggcacgcct
     1381 gatggcattg ctccgtcata tttaaaaacc aggaggggaa ttctgacatc ggttgcatga
     1441 ccgtaacatc cgtccccgga tacaaaacat ccgagtcttc cgattgcata acccatagcc
     1501 atcgtaggcg ccatcccgtc atagtactgt cccagatccc atttgttgct tttcagatag
     1561 taatagatga aaagagttcc agtcagaaac ccgccgtaaa agacaagtcc tccccctgaa
     1621 aaaagggaac tccagagacc gtaactttcc atacatacct ggcaggaagg gtcattgtat
     1681 tctctgaatc cataccagtg tgtcagagga aacatatagt ttcccggaag gacaaatatc
     1741 tgaacccaga tttcaaaaat ataaaagatc ttggaaccta caagtgtacc aagtacccct
     1801 aaaaaaacaa tattgtc
//
LOCUS       DAOQGJ010000853         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46026_length_1814_cov_2.875497, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000853 DAOQGJ010000000
VERSION     DAOQGJ010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46026_length_1814_cov_2.875497"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PK683_12915"
     CDS             <1..427
                     /locus_tag="PK683_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH09394.1"
                     /translation="AYGTKNLQEKFVSILIQPVKDKGILLIRRGIEPGKGRLALPGGF
                     VDFGESWQEAGARELSEEAGVSVPASGISEFRIISSEEGRLVLIFGIGTEILESDMPV
                     FVPSEESTERVVVNSPEELAFPLHTKVLKEYFDHHEKRQ"
     gene            411..1016
                     /locus_tag="PK683_12920"
     CDS             411..1016
                     /locus_tag="PK683_12920"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNH09395.1"
                     /translation="MKKDNKIIIYILFCMIQFCTLRIPSVTFTETPTAAERQMVGTDK
                     NLEKDGWILSSVRTSASGSEIWEKEVLDDTFSDKNLTPEIMASLRRIAYLSAELRDYR
                     KKGFVGETLEGKAAVNPRWKESRDASDFEKSKERINDVLVLMNESRETVYNKKIELLR
                     SEKLSPPDFEARKRQILLRYHSMTEEGEYFESAKGKWTIRQ"
     gene            1154..>1814
                     /locus_tag="PK683_12925"
     CDS             1154..>1814
                     /locus_tag="PK683_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09396.1"
                     /translation="MIYFIILINSYCSVFFYRSGKVDPVEFQYDKIAEAHYGPHNEKP
                     FPLTVRRGNNLYPSLTKDARYLFFASDSNGNFDVYIRDLKKSVVAAVTNHPAPQYKPA
                     VSPDGKKLAFVTERYDSEGDIGLAEINPEKIIEKSIQGEYDPSQFEFALVTNEKLESE
                     GTVLRAADTDPSWSNDSRFIVFATSRFSPGITNIAVIDTANKNKITQITKDGGASPVF
                     SP"
BASE COUNT          540 a          363 c          445 g          466 t
ORIGIN      
        1 agcatatggc acaaaaaatc tgcaagaaaa atttgtatcc attctgattc agcccgtcaa
       61 ggacaaaggg atactcctca tccgaagagg catagaaccg gggaagggga gacttgcact
      121 tcccggagga tttgtggatt tcggggaaag ctggcaggag gcaggggcaa gggaactctc
      181 cgaagaggcg ggggtatccg ttcctgcatc gggcatttct gagttcagaa tcatcagttc
      241 ggaagaggga aggctagtgc tgattttcgg aatcgggacc gaaattctgg aatccgatat
      301 gcctgttttt gtcccttcgg aagagagcac ggaaagagtt gtcgtgaatt ctcccgaaga
      361 gcttgcattt cctctgcaca cgaaagtatt gaaagagtat tttgatcatc atgaaaaaag
      421 acaataaaat tattatatat atattatttt gcatgatcca gttctgcacc ctcaggatac
      481 ccagtgtcac tttcactgag actcccactg ctgcggaacg gcagatggtg gggacagaca
      541 aaaacctgga gaaggacggc tggattcttt cctctgtaag aacctctgct tcaggaagcg
      601 agatctggga aaaggaagtt ctggatgata ctttttccga caaaaatctt acacctgaaa
      661 tcatggcttc tctcaggcgt attgcctatc tgagtgcaga gctgagagat tacagaaaga
      721 agggttttgt cggggagact ctggaaggga aggctgcggt caatcctcgc tggaaggaaa
      781 gccgtgacgc atctgatttt gaaaagtcca aggaaagaat caatgatgtt ctggttctga
      841 tgaatgaatc ccgtgaaact gtttataata aaaaaattga gcttctccgg tctgagaaac
      901 tcagtcctcc ggattttgag gcaaggaaaa gacagatcct tctccgatat cattccatga
      961 ctgaggaagg agaatacttc gagtcggcaa aaggaaaatg gacaatccgc caatgattaa
     1021 taggtatatt ctatcatttt tcaaatggga taaaaaaaaa tgtttttata atgggttcat
     1081 aagattggaa aaagcgggga tctgggggtg gtcagccccc aggaatcccc cggcacggcc
     1141 tcattggagt acgatgatat attttatcat actgataaac tcctactgct cagtattttt
     1201 ctacagatcc ggaaaagtcg atccggtgga atttcaatac gacaagattg cggaagcgca
     1261 ctacggtcct cacaatgaaa aaccttttcc tctgactgta agaagaggaa acaatctgta
     1321 tccttcgctt actaaggatg ctcggtatct tttttttgcg agtgattcca acgggaattt
     1381 tgatgtatat atccgggacc tgaaaaagtc cgtagttgct gcagttacaa atcatccggc
     1441 tccgcagtac aaacctgcag tgagtccgga tggaaaaaaa ctggcatttg tcacagagcg
     1501 ttatgattca gaaggagata tcggtctcgc agaaattaat ccagagaaga ttatcgaaaa
     1561 atccattcag ggagaatatg atccttccca gtttgaattt gcgcttgtca caaatgagaa
     1621 gctggaatct gaagggacgg ttctgcgtgc agcagatact gatccttcct ggtcgaatga
     1681 cagcaggttc attgtatttg ctacatccag attttcgccc gggattacaa atattgcagt
     1741 cattgataca gcgaacaaaa ataaaatcac ccagatcaca aaggacgggg gagcatcgcc
     1801 tgtattttct cctg
//
LOCUS       DAOQGJ010000854         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46083_length_1813_cov_2.666098, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000854 DAOQGJ010000000
VERSION     DAOQGJ010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46083_length_1813_cov_2.666098"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PK683_12930"
     CDS             complement(<1..1404)
                     /locus_tag="PK683_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNH09397.1"
                     /translation="MEHLIFHLIFTVLFIIANVVFARAVIYRLGLVFEGQKAHFNEDA
                     RGNLNLGFRIKSILENVLMQKKNMQEPLRGMMHSFIFYGFLTYLLHTTNQMVAGVAGY
                     WLADPYSFSLLGFSDTISHAYEGLVQIMSFFVLTGLAYFAGRRWIFKAKGLDVHSPAS
                     AIVIGMIGTLMITTLLGEGAKVISETYSIKTGGLREGFPIAVAISHIWSALGVDGKSA
                     DTVFRIMWWGHILTVFTFMLYVPTSKHAHLIFAPINYFFATDTPKGALSKLDLESETA
                     IWGANQVQNFPFPNLLDGLSCIECGRCQVQCPANRTGKVLNPKAIIGDLKHALLEKMP
                     EVQAAKKAGKSAEEIMALDTNVIGNYTSEEAIWGCTTCFACVQACPVGNNQVNAIVEM
                     RRYLVLAESRFPAELQGAFTNMENNSNPWGVGAHTRADWAEGLGVKTMAEDSNVDILY
                     WVGCAGAFDERSKSTARS"
     gene            complement(1519..1644)
                     /locus_tag="PK683_12935"
     CDS             complement(1519..1644)
                     /locus_tag="PK683_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09398.1"
                     /translation="MQTHTHLEWAVFIFIITACLLTASVTAAKLISKEINAKRSK"
BASE COUNT          502 a          412 c          385 g          514 t
ORIGIN      
        1 agaacgggca gtgctcttgc ttctttcatc aaatgctcct gcacatccta cccagtacag
       61 aatgtccaca tttgaatctt ctgccattgt ttttactcca agaccttccg cccagtctgc
      121 tcttgtatga gctcccacgc cccaaggatt ggaattattt tccatatttg taaatgctcc
      181 ctgcagttcc gcggggaatc tggattcagc aagaacaaga tatcttctca tttcaacgat
      241 tgcattgacc tggttgtttc cgacaggaca tgcctgaaca catgcaaagc aggtcgtaca
      301 tccccagata gcttcttcgg aagtataatt cccgatgaca tttgtatcga gtgccataat
      361 ttcttctgcg gactttcctg cttttttagc agcctgaacc tcaggcattt tttccagaag
      421 ggcatgtttc agatcgccga taatcgcttt cggattcaga acctttccgg tcctgtttgc
      481 aggacactgc acctgacatc ttccgcattc gatgcaggaa agaccgtcca gcaggttagg
      541 gaatgggaaa ttctgcacct gattggcacc ccagattgct gtttcacttt ccagatccag
      601 tttggaaagg gcacctttag gagtatcagt tgcgaagaaa taattgattg gtgcaaaaat
      661 cagatgggcg tgcttggagg taggtacata gagcataaaa gtgaatacag tcagaatgtg
      721 accccaccac attattctga agactgtatc tgccgatttt ccgtctacac cgagtgctga
      781 ccagatatga ctgattgcaa ctgcaattgg aaatccttct ctcagtccgc ctgttttaat
      841 gctgtatgtt tccgaaatta cttttgcacc ttcaccgagg agagtagtga tcatcagtgt
      901 tccaatcata ccgatgacaa tcgctgatgc aggagaatga acatccagcc cctttgcttt
      961 gaagatccat ctgcgtcctg caaaataagc aagccctgtc agaacaaaaa aggacatgat
     1021 ctgaaccagt ccttcatagg cgtgcgagat tgtgtccgaa aaaccgagaa ggctgaaaga
     1081 atatggatct gcaagccagt aaccggcaac cccagccacc atctggttag ttgtatggag
     1141 aaggtaggtc aggaatccgt aaaatatgaa ggagtgcatc attcctcgga gaggttcctg
     1201 catatttttc ttctgcatca ggacattttc cagaatgctt ttgatcctga atcccaggtt
     1261 cagatttcct ctggcatctt cattgaaatg agctttctgc ccttcaaaaa cgagtcccag
     1321 tctgtaaatg actgcccgtg cgaatacaac gttcgctatg ataaacaata ctgtaaatat
     1381 tagatgaaat atcaggtgtt ccaagtcctt gcctctcgac tagattctga atctaggaaa
     1441 aaagagatga atcaagtgtc gagtaaaaaa aggattttcg agtctcagtc cgcaaaggca
     1501 gaagaagcct gtcattgctc atttagatcg ttttgcatta atctctttgg atatcagttt
     1561 tgcagcggtc acagatgcag tgagaagaca tgctgtaata atgaagatga aaactgccca
     1621 ttccagatgt gtatgtgtct gcataatccg ctgcctctta tcgcagcgta acaccgaaaa
     1681 aaaattggtc aacagttttt cggaaataaa atgcctgcct ttgctctgct caaaagcatt
     1741 taaatctctc gttctttggg ctgcggaggt aaattcattt gtgaataaaa caatattgca
     1801 aagggttaaa att
//
LOCUS       DAOQGJ010000855         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46121_length_1812_cov_3.056346, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000855 DAOQGJ010000000
VERSION     DAOQGJ010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46121_length_1812_cov_3.056346"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1812
                     /locus_tag="PK683_12940"
     CDS             <1..>1812
                     /locus_tag="PK683_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH09399.1"
                     /translation="LGRGLERSQRRKVEEEKNQLLKSRDKIGENLDNLSKIIRSEKGK
                     QLLDKIKGSRESFIRAQDNYIKAIALNDKDLAYTVLINELRPVQLEYQLNIDNMNEFQ
                     SEMVKREGKEGTQAAYSTVLSTIIAMLLTGILLAFAARYLVNAIVKPIVEMEKAVTEV
                     GNEGRFDINIEVKSNDEIGSFAKAFKNMMAAFQSMLGDSVMLTEAAKQGSLSRRADSA
                     KYKGDIRKIVEGVNQTLDAVIGPLNVAADYVDKISKGEVLPKITENYNGDFNDIKNNL
                     NTLIHAVETRGSDIESLIVTAINGKLDVRFDLSKYHGYNKTQMQGINRMLDALISPLN
                     VAADYVDKISKGTIPPRITDTYNGDFNTIKNNLNSLIDTLNSFTEAQIRLTELHHEKG
                     LIDEKINSSRFNGVYAGMAENINSLVEQHIAVKMRVVEIVSRYSQGDLSIDMEKLPGK
                     KAKITESIDAVKMNMLSLNREILALSQSAIQGNLSARADAGKFQHMYKEMISGINKTL
                     DAVIGPLNIAADYVDKISRGAIPQRITETYNGDFNTIKNNLNRCIDNVNAMVNDAAML
                     SRAAVEGKLATRADASKHEGDFRKIVEGVNQTLDSVI"
BASE COUNT          639 a          347 c          413 g          413 t
ORIGIN      
        1 atcttgggcg cggccttgaa cgaagccaaa gaagaaaagt cgaagaagaa aaaaatcagc
       61 ttctgaaaag cagggataag atcggagaga atctggacaa tctctccaaa atcatccgat
      121 cagaaaaagg aaagcagctc ctcgataaaa taaaaggatc cagagaaagc ttcataagag
      181 cacaggataa ttatattaaa gcaatcgccc tgaatgacaa agaccttgcc tatacagttc
      241 ttatcaatga gctcagaccc gttcagctgg aatatcagtt gaacatagac aatatgaatg
      301 aatttcagtc cgaaatggta aaaagggaag gaaaggaagg aactcaggcc gcctactcta
      361 ctgttctatc aacgatcatt gcaatgcttc tgacaggaat ccttctggca tttgcagctc
      421 ggtaccttgt taatgcaatt gtaaaaccga tagtggaaat ggagaaagct gtaactgagg
      481 taggaaatga gggcagattc gatataaata tagaagtcaa gtccaatgac gaaataggca
      541 gctttgcaaa agcattcaaa aatatgatgg ctgcatttca gtcaatgctg ggtgattccg
      601 tgatgctgac agaagcagcg aagcagggca gcctgagcag acgtgccgac agtgcaaagt
      661 ataaaggtga tatcaggaag attgtggaag gagtcaacca gactctcgac gcagtcatag
      721 gacctctgaa tgtagctgcc gattatgtgg ataaaatctc gaaaggtgag gtgcttccta
      781 aaattactga aaattataat ggagatttca acgacatcaa gaataacctg aatacgctaa
      841 ttcatgcagt tgaaacaaga ggttcggaca ttgaaagtct gattgtcact gcaatcaacg
      901 gaaaactcga tgtgcgtttt gatctctcaa agtatcacgg atacaacaag actcagatgc
      961 agggcatcaa cagaatgctc gatgcactga tatcacctct gaatgttgca gcagactatg
     1021 tggataaaat atcgaaagga actattcctc caagaatcac cgacacttac aacggagatt
     1081 tcaacacaat caagaataat ctgaattcac tgattgatac gctgaacagc ttcacggaag
     1141 ctcaaatcag acttacagaa cttcatcatg aaaaaggtct gattgatgaa aaaataaatt
     1201 cctccagatt taacggagtt tatgcaggaa tggcggaaaa tatcaactct cttgttgagc
     1261 agcatattgc agtcaaaatg agagttgtgg aaattgtgtc cagatacagt cagggggatt
     1321 taagcatcga tatggaaaaa cttcctggaa aaaaagctaa gattactgaa tcaattgacg
     1381 cagtaaaaat gaatatgctc agtctgaacc gggaaatcct tgcactttct cagtctgcca
     1441 ttcagggaaa cctttctgcg agagccgatg caggcaaatt tcagcacatg tacaaggaaa
     1501 tgatttccgg aataaacaag accctggatg cagtcatcgg acctctgaat atagctgcag
     1561 actatgtaga taaaatatcc cggggagcca ttcctcagag aatcacggag acttacaacg
     1621 gagacttcaa cactatcaag aacaatctga acagatgcat tgacaatgta aacgcaatgg
     1681 tcaatgatgc tgcaatgctg tcgagagcgg cagtggaagg caaacttgct acgagggcag
     1741 atgcttcaaa acatgaagga gacttcagaa agatagtgga aggagtcaat cagactctgg
     1801 actcggtcat tg
//
LOCUS       DAOQGJ010000856         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46210_length_1810_cov_2.452991, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000856 DAOQGJ010000000
VERSION     DAOQGJ010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46210_length_1810_cov_2.452991"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1810
                     /locus_tag="PK683_12945"
     CDS             <1..>1810
                     /locus_tag="PK683_12945"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNH09400.1"
                     /translation="GSITGRTVRKKVTGTPAVPLSEVEVLADITGKTFKIKSSSDGTF
                     SLAVSGAVRGSGTTAFFRKDSFAESARAITFDIPNLRVDLGDVEVLTQDEANSLPLGT
                     SDSGTGPIVKGQIIDDFTSNPLDQASINISIASGTTLPAVTDSTGSFTIQNSKLKAGS
                     SYNITVSKQNYLESSNVTVNVTGDTNTINGNPIRLYRAYGNITGTVIDDSGAPLAGVS
                     VSAVDSKGATASGATDSSGNFTLTSNAFLLGSDYTLSITKSGYSSLTSTVPISITGNN
                     TLTPNPFVLTISGTISGTVGTASCGNVTLSFTDSNGNPVSTTVTGGSTYTLNNTLTAG
                     QNGIKKGQSYSITAVSSGSPGCETAVVNTSAVTEGTNSLNISLTARVACGGGNTCIAG
                     KVVDASDTASALQGITVSTTDSVGTTRTATTSAAGTFSLAGTFNNGATFSLSVSASGY
                     TGAASTAGQTIPVQVTVAAGLTHNIVHNGDSTLFNLATSDIKLYPIGISATIGGSKKK
                     FYQGVKQTYEKFLTEKWGFTVSGRDVTDLNTASSFYIHTDDVSQPPSSPGSYCSGYTY
                     RHSNIMAVNGNVSKGVIQESNNDPRACNTPIRGATGG"
BASE COUNT          535 a          445 c          407 g          423 t
ORIGIN      
        1 acggaagcat tacaggcaga acagtcagaa aaaaagtgac aggcacgcct gcagtccccc
       61 tttcagaggt ggaggtcctg gctgacatta ccggaaaaac attcaaaatt aaatcttctt
      121 cagacgggac attcagtctg gcagtcagcg gagctgtgag aggctcaggc accactgctt
      181 ttttcagaaa ggacagtttt gcagaatcag ccagggcaat tacctttgat attccaaacc
      241 tgagagtgga tcttggagat gtggaagtgc tgactcagga tgaagcaaat tcacttccgt
      301 taggcacctc tgattccgga acagggccta tagtcaaggg acagattatt gatgatttta
      361 cgagcaatcc cctcgatcag gccagcataa atatttccat tgcatccggc acaactcttc
      421 cggcagtcac agacagcact ggatcattta cgatacagaa ctcgaaactc aaagctggga
      481 gttcctacaa tatcactgtc agcaagcaga attatctcga atcctccaat gtaactgtga
      541 acgttacagg agatacaaat acgatcaacg gaaacccgat ccgtctctac agagcctacg
      601 gaaacattac aggaacagtc attgatgatt caggagcccc tcttgcaggc gtctctgtat
      661 ctgcagttga ttccaaagga gccactgctt caggagccac tgattccagc gggaatttca
      721 ctctgacgag caatgccttt cttcttggaa gcgattatac tctcagcatc acaaaatcag
      781 gatactcgag ccttacatcc actgtcccga tttccatcac tggaaacaat actctgactc
      841 cgaacccctt tgttctcaca ataagcggaa cgatttcagg aacagtcgga actgcatcct
      901 gcggaaacgt tactctctcc tttaccgatt cgaacggaaa tcctgtcagt acaacggtta
      961 caggcggatc aacgtatacc ctgaacaaca cattgacagc aggacagaac gggatcaaaa
     1021 aagggcagag ttattccatc actgcagtca gcagcggatc tccaggatgc gaaacagcgg
     1081 tcgtaaatac atcagcagtc accgaaggca caaacagtct gaatatcagt ctgactgcaa
     1141 gggtagcctg cggaggcgga aacacatgca ttgcaggaaa ggtagttgat gcatctgata
     1201 ccgcatcggc tcttcaggga atcacagtca gcacgactga ttcagtggga accacgagaa
     1261 ctgccactac aagcgcagca gggacgtttt cactggcagg cacattcaat aacggggcta
     1321 cgttcagtct ttcagtctct gcatcaggat atacgggagc cgcaagcaca gccggtcaga
     1381 ctataccagt tcaggtgaca gtggctgctg gattgactca taatatcgtt cataacggtg
     1441 attcgactct ttttaatctg gcaaccagcg acattaaatt atatcccatc gggatcagtg
     1501 caacaatcgg cggttccaag aaaaaattct atcagggagt caagcagact tatgaaaaat
     1561 ttctcactga gaaatggggc tttactgttt caggaagaga tgtcactgat ctcaacactg
     1621 cctccagttt ttatattcat acggatgatg tttcacaacc cccttcatct cccggttctt
     1681 actgttccgg ttatacatac cgtcattcaa atataatggc tgtaaatgga aatgtttcaa
     1741 aaggagtcat acaggaaagc aataatgatc cgagagcctg caacacgcct atcagaggtg
     1801 ccacaggcgg
//
LOCUS       DAOQGJ010000857         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46244_length_1809_cov_2.828392, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000857 DAOQGJ010000000
VERSION     DAOQGJ010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46244_length_1809_cov_2.828392"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PK683_12950"
     CDS             complement(<1..1233)
                     /locus_tag="PK683_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09401.1"
                     /translation="MRTGSSFWTSKLLISVLALFLIQSSEFKFGSDSDHKFQGLKKNK
                     VIVVKTGVNDFRLELAKNKHKSTELFLDFENKNSSDLKDLNGNYEINDSLYSVSEGKT
                     MYGKRYASFTTRESQIRIRSNPTRLLSKTVISEPFYFSFFIMPGEVEQSSTIFSKTYI
                     TGAKKYGIECKVANSRTEFVFHNLFSYRKGETRSFTLSSSDRLKSKVWTHITITYDPQ
                     SGEGKLFEDGVEKSEFHAVLSSAEPTALLAGFHPNDTSYLVIGKGFYGKLDNFLIGRG
                     ELPDVSKMTQPYDKVDYNENIQFVNHTKGYAVSKVMKTENSKAVPLELVYKTNVPEGT
                     HLEILYRFSEEPFSEEDKEPVWTHYDPETFKAGAEASPFQFFQWKAILRSDYSGKKTP
                     SLTSLTLKYRKTVPPSQTF"
     gene            complement(1202..1645)
                     /gene="rpiB"
                     /locus_tag="PK683_12955"
     CDS             complement(1202..1645)
                     /gene="rpiB"
                     /locus_tag="PK683_12955"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNH09402.1"
                     /translation="MEGSVRKKIGVVSDHGGFRLKEFLKNSLSEKHEVVDEGVYEEKS
                     ADYPTVVKSACENLLAGKFDLLIAVCGTGIGASIAANRIKGIRAALCHDEFTAEMSKR
                     HNNANVLVLGGRVLAEDLSARITDKWLTAEFEGGRHENRIQLLDK"
BASE COUNT          449 a          382 c          373 g          605 t
ORIGIN      
        1 gaatgtctgg ctaggaggaa ctgtttttct gtatttcagc gtgagtgaag tcagtgaggg
       61 cgtttttttt ccggagtagt ctgatctgag aattgctttc cactgaaaaa actgaaatgg
      121 ggaggcctct gccccggcct tgaacgtttc cgggtcataa tgggtccata caggttcctt
      181 gtcttcttcg gagaagggtt cttctgaaaa tctgtacaga atttccagat gagtgccttc
      241 gggaacatta gttttgtata caagttcaag aggtacagcc tttgagtttt ctgtcttcat
      301 tacttttgac acagcatacc ctttagtatg atttacaaac tgtatgtttt cattataatc
      361 tactttatcg taaggctggg tcatttttga tacatccggc agttctcccc ttcctatcag
      421 aaagttgtcg agttttccgt aaaaaccttt tcctatcaca aggtaggatg tatcgtttgg
      481 atgaaagcct gccagcaggg ctgtcggttc cgcactgctg agaactgcgt ggaactccga
      541 tttttcaaca ccgtcttcaa acagttttcc ttcgccgctc tggggatcgt atgtgattgt
      601 gatatgtgtc caaacttttg atttcagtct gtcggaactg gaaagagtga atgatctagt
      661 ttcgcctttt ctgtaggaaa acaaattatg aaagacgaat tccgttctgc tgtttgccac
      721 cttgcattct attccgtatt tttttgcacc ggtaatgtat gtcttcgaaa agattgtgga
      781 actctgttcc acttcaccgg gcataatgaa aaatgaaaag taaaacggtt cagaaattac
      841 tgtctttgaa agaagtctcg tcggattgga tctgattctt atctgactct ctctcgttgt
      901 aaatgaagca tatctctttc cgtacattgt cttgccctca gagactgaat agagagaatc
      961 attgatttca taattcccgt tcagatcctt gaggtcggat gagtttttat tttcaaagtc
     1021 aagaaacagt tctgtgcttt tatgcttatt cttggcaagt tcaagcctga agtcatttac
     1081 gcctgttttc actacgatga ctttgttttt tttcagaccc tgaaatttgt ggtctgagtc
     1141 gcttccaaat ttaaattcgc tggactgaat cagaaaaagg gcaaggactg aaatgagaag
     1201 tttacttgtc cagaagctgg atcctgttct catgtctgcc tccttcaaat tctgctgtca
     1261 gccatttatc cgtaattctt gcggacaggt cttctgcaag gactcttcct cccagaacca
     1321 gtacgttggc attattgtgt ctcttgctca tctctgcagt gaattcatca tgacacagtg
     1381 ctgctctgat tccttttatc ctgttcgcag caatggaagc tcctattcct gttccgcaca
     1441 ccgctatcag caggtcaaac tttccggcaa gcaggttttc gcaggcagat tttaccacag
     1501 ttgggtaatc ggcagatttt tcttcgtaca ctccttcatc tacaacttca tgtttttcgg
     1561 acaatgaatt tttcaggaat tctttcagtc tgaatccgcc atgatcagag actacaccaa
     1621 ttttttttct cacagatccc tccatttaaa gtatcggtaa gaattctctg aaaattgtac
     1681 tggacttgag tttcagatca ggaaatttat acaaatacag ggaattcgaa tgaaaaaaat
     1741 caaattgtcc agagggaaag ttccgatttt tgaagatgct gactccgatt gctgcagtcc
     1801 atcctcagg
//
LOCUS       DAOQGJ010000858         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46245_length_1809_cov_2.773660, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000858 DAOQGJ010000000
VERSION     DAOQGJ010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46245_length_1809_cov_2.773660"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK683_12960"
     CDS             <1..195
                     /locus_tag="PK683_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09403.1"
                     /translation="IRKPRYIYNQHRYCILKLYFEDGTERLLGSRFGNVDDDGSSYFI
                     YIHYPEKLDKRIIHRVYEIR"
     gene            311..496
                     /locus_tag="PK683_12965"
     CDS             311..496
                     /locus_tag="PK683_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09404.1"
                     /translation="MISVKGRYENGKIELNQHPEIQCGRDVIVTFLEEKLNEDSFWDL
                     LNLIDWNQEDTCKERCT"
     gene            complement(609..1406)
                     /locus_tag="PK683_12970"
     CDS             complement(609..1406)
                     /locus_tag="PK683_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09405.1"
                     /translation="MYRLTKPQTPADEKKLGVVAFGLYLVNPPGDGALSKLFNPNDAI
                     YTVLVSDHKLVKVESINKPTTSAKTSPYTLKPPVAAGKEGDSELFMADGVSFSGFSAY
                     AMENSSDKYAIGEVSYVYCYNQTRMVNGVMQTYRQCDQIVYPVDPYTSYTAFPIDPKA
                     GDVQFLGLFQARIIKTVKTDPYGRNNAGLLSVAGMGDYERIQIMPDDGYIIKSGNTDL
                     IKSYYGTEAQTLKGAEINFLKNFILNQKDGFWKEKAELKLKKLGGKK"
     gene            1627..>1809
                     /locus_tag="PK683_12975"
     CDS             1627..>1809
                     /locus_tag="PK683_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09406.1"
                     /translation="MKIQEIRKQKGKKVFIFRSIRKDGYILDSIKIHINSKSRILQMD
                     IEELKLGSCSFGIRGRL"
BASE COUNT          576 a          329 c          344 g          560 t
ORIGIN      
        1 atccgaaaac ctaggtatat atataatcag cacagatact gtatactgaa actgtatttt
       61 gaagacggaa cagaaaggct tctgggatca agatttggca atgtagacga tgatggatcg
      121 agttacttta tttatataca ttatcctgaa aaacttgata aaagaattat tcatagagtc
      181 tatgaaattc gatagaggtg ttaaactgca gactggagtt taacgacttg atctttctgc
      241 tgaaattctg tttataaaaa aatgttgccc cgatcttttg cgggatacaa aactgataca
      301 ggagcggatt atgatctcag tgaaaggcag atacgaaaac ggaaaaattg aattaaatca
      361 gcatcctgaa attcaatgcg gcagagatgt aatcgtgacc tttttagagg aaaaattgaa
      421 tgaggattct ttttgggatt tactgaacct gatagactgg aatcaggaag atacctgcaa
      481 ggagcgatgt acttaatcgt tggatccgtt agataaaaaa tattctatac ttattttata
      541 attcactcac ccagctgtga ctgaatctgg atgagtatga tactgtcata gacaaactta
      601 agactgtatt actttttccc tcccagtttt ttcaatttca actcagcttt ttctttccag
      661 aaaccgtctt tttgatttaa tataaagttt ttcaaaaaat taatttccgc acccttcagt
      721 gtctgggctt cagttccata gtaagacttt attaaatcag tatttccgct tttaatgata
      781 tatccgtcat ccggcataat ctgaattcgt tcatagtccc ccattcctgc aactgaaaga
      841 aggcctgcat tattccttcc atatggatct gtcttcacag tctttatgat tcttgcctga
      901 aataagccta aaaactggac atctcctgcc tttgggtcaa taggaaatgc agtgtacgat
      961 gtataaggat cgacaggata aacgatctga tcacattgtc gataggtctg cattacgccg
     1021 tttaccattc tggtttgatt gtaacagtag acataggata cttcaccgat ggcatatttg
     1081 tcggagctgt tttccatcgc gtatgcagaa aagccgctga acgacacacc atctgccata
     1141 aaaagttcgc tgtcaccctc ttttccagca gcaactggag gcttgagagt ataaggtgag
     1201 gttttcgcag aagttgtggg tttattgata ctctcaactt tgaccagttt atggtcagaa
     1261 acaagaacag tataaattgc atcattcgga ttaaataatt tagagagagc tccgtctccc
     1321 ggcggattta ccaagtataa cccaaaagca actacaccca attttttttc atccgcagga
     1381 gtctgcggct ttgtcaatct gtacattttc ggtgcgcatg cattcaaaaa gagaaataga
     1441 actaaaaagc ctattttttt cataaaataa cctcacaaat ttttcagtca ggataatata
     1501 ttctttttct gggtaaagat tttttattca ctcataaggt tattttttct gtttgattta
     1561 tttcaataat gctgttaaat aaattagaca aatcaatgat cctagaaact gaggaaatcc
     1621 gaggaaatga aaattcagga aatcagaaaa caaaaaggga agaaagtatt catcttccga
     1681 agcattcgaa aagacggata tatcctcgat agtataaaga ttcatataaa cagtaaaagc
     1741 aggattctcc agatggacat tgaagagctg aagctcggct cctgcagttt cggtatccgg
     1801 ggcaggctg
//
LOCUS       DAOQGJ010000859         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46252_length_1809_cov_2.637970, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000859 DAOQGJ010000000
VERSION     DAOQGJ010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46252_length_1809_cov_2.637970"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PK683_12980"
     CDS             complement(<1..501)
                     /locus_tag="PK683_12980"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH09407.1"
                     /translation="MNFTYIMIFSMMIWGVSWVAGKIISSNADQFTVLFWRNILTSVS
                     LFPVIFILKLELKLTLKTVIMSFLASMAMLAYSYLFFLGLQKGMAGAAGVLVTSLNPI
                     MNFTLVCIFNRHKLSRNEALGLVLGAAGGLIQMKIWNESMGSLYEKGIFLFLSASFLW
                     AVLSFIT"
     gene            complement(498..1574)
                     /locus_tag="PK683_12985"
     CDS             complement(498..1574)
                     /locus_tag="PK683_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH09408.1"
                     /translation="MKALRIALIQLFSLKREPKLLFMIFLLPAFMLYTVYAGYSASFR
                     LGTYKIGIQKNQAYDSGLLEKIRKSVHENGRPVFSVIETEASDSDRLIEEKAVDLIFL
                     PEKKDGKNEFILLGDGSSVRFSKAGMFLGSVLYEYNVNIIPYKVNRKNFFKNVPETDF
                     QIAVPGMMVFAVLLLIPQTAILAGRDRRSGILDRYTNAGVSPFQYFTGLCISQAVCGF
                     LQLIFMLLISVFMGFRISQMNLIIFFISGILLIFASASMGVLLSGFIKDDSEALNAGS
                     AVSMVQVFLSGAFFAFPGKILFMLRDTVWNVWDLIPATEAVLIWNAGFGYGISSIQRL
                     VWLSVLAVIWYIFGYKIYELKEKK"
     gene            complement(1571..>1809)
                     /locus_tag="PK683_12990"
     CDS             complement(1571..>1809)
                     /locus_tag="PK683_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH09409.1"
                     /translation="PQSRILVREYIRSLAGTKTVLLTTHDMDEADRLSGRIAIIDQGK
                     LLRLGTPSELKNDFGRNVSLEDVFIGLTGRRLRE"
BASE COUNT          558 a          415 c          353 g          483 t
ORIGIN      
        1 ggtaataaaa cttagaactg cccagagaaa actcgcagac agaaaaagaa agatcccttt
       61 ctcataaaga cttcccatgc tttcattcca gatcttcatc tggatcagtc ctccggcagc
      121 gcccagaaca agaccgagag cctcgtttcg gctcagctta tgcctgttga aaatacacac
      181 taatgtaaaa ttcatgatag gattgagaga ggtcacgaga acacctgcgg cgcctgccat
      241 tcccttctgc agtccaagga agaacagata cgaataggca agcattgcca tggaagccag
      301 aaaactcatg atgactgtct ttaaggtgag tttcagttcc agcttcagaa taaagatcac
      361 cggaaatagg gaaacagacg taagtatgtt tctccagaaa agaacagtaa actgatctgc
      421 gtttgacgag atgattttcc ctgcgaccca gcttactccc cagatcatca tcgaaaaaat
      481 cattatatat gtgaagttca tttcttttcc tttaattcat atattttata tccaaatata
      541 taccagatta cggctagaac ggatagccag accagtctct gtatgctgga tattccgtat
      601 ccgaatcctg cattccatat cagaacggct tccgtggcag ggatcagatc ccatacattc
      661 catactgtat ccctcagcat aaacagaatc tttccgggaa atgcaaaaaa tgcgcctgag
      721 agaaagacct gcaccattga aacagctgaa ccggcattca gtgcctccga gtcatccttt
      781 atgaatccgg agagaagaac tcccattgag gctgatgcaa aaatcagaag aattcctgaa
      841 atgaaaaaaa tgataagatt catttgtgaa attctgaatc ccataaaaac actgatcaga
      901 agcataaaaa tgagctgaag aaaaccgcag acagcctgag aaatgcacag gcctgtgaaa
      961 tactgaaacg gtgagacccc tgcatttgta tatctgtcca gtattccgct tctcctgtct
     1021 cttcctgcca gaatggctgt ctgaggaatc agcagaagaa cagcaaagac catcattcct
     1081 ggaactgcga tctgaaaatc agtttcaggc acatttttaa aaaaattttt tctgtttact
     1141 ttataaggaa ttatatttac attatattcg tataggacag atcctaaaaa cattcctgcc
     1201 ttgctgaacc ggacagagct tccgtcaccc agaagaatga attcattttt cccgtctttc
     1261 ttctccggaa gaaaaatcag atcgaccgct ttttcttcga tcagtctgtc tgaatcggaa
     1321 gcctctgttt caatgacgga aaatacaggt cttccatttt catgaacaga cttacgaatt
     1381 ttctccagaa gcccagaatc atatgcttgg tttttctgaa tgccgatttt atatgtgcct
     1441 agccggaaag aggcagaata gcctgcatac acagtgtaca gcataaatgc aggaagcaga
     1501 aaaatcataa aaagaagctt tggttctctt ttcagactga aaagctgaat cagtgcaatt
     1561 ctcagagctt tcattctctg agccttcttc cagtcagtcc gataaagacg tcctccaggc
     1621 tgacattccg accgaagtca tttttcagct cagaaggagt tcccagcctg agaagttttc
     1681 cctgatcgat gattgcaatt ctgccggaaa gcctgtctgc ttcatccata tcatgagtcg
     1741 tcagaagcac tgttttagtt cctgcaaggg aacggatgta ttctctgaca agtattctgc
     1801 tctgcggat
//
LOCUS       DAOQGJ010000860         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46274_length_1808_cov_4.549344, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000860 DAOQGJ010000000
VERSION     DAOQGJ010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46274_length_1808_cov_4.549344"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK683_12995"
     CDS             <1..201
                     /locus_tag="PK683_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HNH09410.1"
                     /translation="YTGDFNGDGYRDMAVSAYGYFTNSGAVYIFHSAGSSGITAGSPT
                     GADTVLTGSSTNDNFGAALSEH"
     gene            complement(255..467)
                     /locus_tag="PK683_13000"
     CDS             complement(255..467)
                     /locus_tag="PK683_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002749163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HNH09411.1"
                     /translation="MSDIIKDIDRGKGEVLRIEISEYKGKKLLNLRIWYTDKEGELKP
                     TQKGVTVSPESYSELKEAILEAGGRF"
     gene            complement(467..1399)
                     /locus_tag="PK683_13005"
     CDS             complement(467..1399)
                     /locus_tag="PK683_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016759446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNH09412.1"
                     /translation="MKQKREPKINLFKKSAPLIAKVLQNIQLTPEPGKGKRPKKEGIA
                     SIHRIIFEIDHSKYPYVIGQSAGIIPPGKDPEKVAKGSENTDYTVRLYSISSPTHSMG
                     MKEDSIELIVKRDNSYDAEGNITHEGVASNYICNLKPGDEVVMTGPSGKSFLLPDSDF
                     SGDIYFCATGTGIAPFYGMTYELLEHNLIKFTGNIYLIYGAPYSDEIVLNDEFEALAK
                     KHKNFHFIKAVSREEKNPYDGGKMYIPHRVKELGDVLKKSFENGGRMYICGGPKGMEK
                     GVITELQKILGDTGDYEAFESRLEEQKQLFVETY"
BASE COUNT          437 a          435 c          380 g          556 t
ORIGIN      
        1 tatactgggg attttaacgg ggacggctac cgggatatgg cagtatctgc ttacgggtat
       61 tttaccaact ccggagcggt gtatatattt cattctgcag gatcttcggg aatcactgcc
      121 gggtcgccga cgggtgcaga cactgttctg actggttctt ctacaaatga caatttcgga
      181 gctgcactgt ccgaacactg attcaggaca ggctgacagc agaaatgcag tcagccaaaa
      241 agcagatttt taattcagaa acgtccccct gcttcgagaa ttgcttcctt cagctcactg
      301 taactttcag gagatacagt gacccctttc tgagtgggct tcagttctcc ttccttgtca
      361 gtgtaccaga ttctgagatt cagaagtttc tttcccttgt attcagagat ctcaatcctg
      421 agaacctctc ccttgcctct gtcaatatcc ttgattatat cactcatcag tatgtctcca
      481 cgaacagctg tttctgttct tccagtctgg attcaaaagc ctcatagtcc cctgtatcgc
      541 caaggatttt ttgcagctct gtaattacac ctttctccat acctttagga ccgccgcaga
      601 tatacatcct tccgccgttt tcaaaagact tcttaagcac atctcccagt tctttcactc
      661 tatgagggat gtacattttt ccgccatcgt aaggattctt ctcttcgcgg ctcactgcct
      721 taatgaaatg aaagtttttg tgctttttgg caagagcctc aaattcatca ttcagaacaa
      781 tttcatctga ataaggtgca ccgtatataa ggtatatatt tccagtaaat ttaattaaat
      841 tgtgttcgag cagttcgtag gtcattccgt agaatggtgc aatcccggtt ccggtcgcac
      901 agaaataaat atccccggaa aaatcagagt cggggagaag gaaacttttt ccgcttggac
      961 cggtcattac aacttcatcg ccgggcttca gattgcatat gtaatttgat gcaacgcctt
     1021 cgtgagtgat atttccctcc gcatcataac tgttgtctct tttgacaatc agctcaatcg
     1081 aatcttcctt cattcccatg gaatgagtag gggaggagat ggaataaaga cgcacagtgt
     1141 agtcagtgtt ctcagaaccc tttgccactt tttcaggatc ctttcctgga ggaatgattc
     1201 cggcactctg gccgatgaca tacggatatt tagagtgatc aatttcaaag atgattctgt
     1261 ggatgctcgc tatcccttct tttttgggac gtttgccttt ccccggttca ggcgtgagct
     1321 gaatgttctg cagaactttc gcaatgagag gtgcagactt cttgaacaaa ttgattttag
     1381 gttctctttt ttgtttcata acagtcatta tatttcattt gaccctcccg taaaggcatt
     1441 ttctgtggta gatcctacat tgtaagtgac ataatgagag attatgtcgc atatatccgg
     1501 ttttaggccg ggggattcgg cgtgaaccga cctcggatgc aggaactctc acagcacgcc
     1561 gaccgggctt tcggctctcg tccatttttc gcatccgtaa aaaacagacg ccgcttcgct
     1621 gcctcaatcc ctatcccggt tcctatcctg gtacaagatt cagtttgagg agccactgag
     1681 atgctttctg atcccaaggt cccagtcaaa gtccttttcc caatgcaggc ttttcattca
     1741 caggactaga tcgtttctgc tgaactggga cagaacagcc aaaaaattat tttcagaccg
     1801 gttgaaag
//
LOCUS       DAOQGJ010000861         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46288_length_1808_cov_3.217342, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000861 DAOQGJ010000000
VERSION     DAOQGJ010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46288_length_1808_cov_3.217342"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>1808
                     /locus_tag="PK683_13010"
     CDS             129..>1808
                     /locus_tag="PK683_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH09413.1"
                     /translation="MRLFILITAAAVSLSCFEGKKNIPESGSGMMDLRLWDKTRQSSL
                     KLKNDWEFFEGNFFSVSFLNRNPSIAGRKYSLNSLMNVFSLYNSSGTFRMKLQLPETL
                     PQELYIRISGWNSNYILYGGEKALLQSGRTGSTAESSIPWMDSKIFPLGKQEPELELL
                     IHSSNFHSFLPGFWGTVEIGTLRGLELSSGIDFCISFTVTLLFLLTAVYSFLRFRTHR
                     FLQLGAASLCLSFLSAFSGKFIFSTLLTESDLMSLLIKIKMIAKISLFPLLILILRDL
                     IPSSKKHHTVLKTFLVLSCSAFLVFFDALTELNIGQLMLPAMLVPIMFALSLNYRRSR
                     TPVWSETFIDSTKEELEKARTELKEYKKSVESKIIDRSYNMISYMEKLEIQTNEIEKL
                     NELVVNLLAGSNMNEMLHLIFSHIINFYKAEIAFLYFLDTEKNELFPYRGMTKDIPEE
                     IAEYMNTSRIPLSREAGLSYTVYKKRKTVYRKNSKTKYVRRRENAGDGFHPELLSTLY
                     IPIIIKGDFHGIFFLATFNKELNLNKNKLKFISMFTSQLAAAIEKEQILSQM"
BASE COUNT          527 a          388 c          348 g          545 t
ORIGIN      
        1 atgataagtt ttaccgtatg gaacaggaag acctgctatc tgcttttcag tttttaaaat
       61 ttaaaacctg cttgacagtt ttttatgaga aacgatattt agccgacttc aaggagggat
      121 tcctgacttt gcgtttattc attctgatta ctgctgccgc tgtcagcctc tcctgctttg
      181 aaggcaaaaa aaacattcct gaatcaggtt caggaatgat ggatctgcgt ttatgggaca
      241 agacccggca aagttccctg aaactgaaaa atgactggga gtttttcgaa ggaaatttct
      301 tttcagtcag ttttctgaac cgaaatcctt cgattgcggg cagaaaatat tcattgaaca
      361 gcctgatgaa tgttttctcc ttatacaatt cctccggaac cttccgaatg aaactgcagc
      421 tcccggaaac gctcccgcaa gagctctata tacgcatcag cggctggaat tcgaactaca
      481 ttctctacgg gggcgaaaaa gcccttctcc agtccggcag gacaggctcc acggcggagt
      541 cctcgattcc gtggatggat tccaaaattt ttccattagg aaaacaggaa cctgaacttg
      601 aacttctgat tcactcctcc aattttcatt cttttctgcc cggattctgg ggaactgtcg
      661 aaatcggtac tttaaggggg ctggaactgt cctctggaat tgatttctgc atcagtttta
      721 cagtgactct tttgttcttg ctgactgcag tctattcctt tctgagattt cgcacccatc
      781 gttttctgca gcttggtgca gcatccctct gtctctcttt tctttccgct ttttcaggaa
      841 agttcatttt cagcacactg cttactgaat cggatctgat gagtctgctg ataaaaataa
      901 agatgattgc aaaaatcagc ctttttcctc ttctgattct tatcctcaga gatctgattc
      961 cttcatctaa aaaacaccat acagtcctga aaacctttct tgtcctttcc tgctcggctt
     1021 ttcttgtttt ttttgatgca ctgacagaat tgaacattgg tcagctgatg cttcccgcaa
     1081 tgctcgttcc gataatgttc gcgctgtctc tgaattaccg caggagcagg actcctgtct
     1141 ggtcggaaac gtttatagac agcaccaagg aagaacttga gaaggccaga actgaactga
     1201 aggaatataa aaaaagtgta gaatcaaaaa ttatagacag atcatataat atgatatctt
     1261 atatggagaa gctggaaatt cagaccaatg agattgaaaa actgaatgaa ctcgtagtca
     1321 atcttcttgc cggttccaat atgaatgaga tgcttcattt aatattttca catataataa
     1381 atttctataa ggctgaaatt gccttccttt atttcctgga tacggaaaaa aatgagctct
     1441 tcccatatcg cggaatgaca aaggatattc cggaagaaat tgccgaatat atgaacacaa
     1501 gccgcattcc gctttcgaga gaggctggtc tttcctatac cgtttacaaa aaaaggaaaa
     1561 ctgtataccg gaagaattca aagacaaaat atgtcagaag aagagaaaac gccggagacg
     1621 gatttcatcc tgaacttctt tccacattgt atattcccat cattatcaaa ggagattttc
     1681 acggaatttt ctttctggct acattcaaca aagaactgaa tctgaacaaa aataaattaa
     1741 aatttatttc aatgttcaca agccagcttg ctgccgccat agaaaaagaa cagatcttaa
     1801 gtcagatg
//
LOCUS       DAOQGJ010000862         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46295_length_1808_cov_2.802624, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000862 DAOQGJ010000000
VERSION     DAOQGJ010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46295_length_1808_cov_2.802624"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK683_13015"
     CDS             complement(<1..307)
                     /locus_tag="PK683_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09414.1"
                     /translation="MKIKIDLLPGEEMLFELEADFWSRGNNPITKIVGNFFRIIAKIM
                     GMRVRGFLTVTNKRIIETREDVICYCITSGRHTKVVTKESVKEVGYEMTKMCGLCCDS
                     "
     gene            579..>1808
                     /locus_tag="PK683_13020"
     CDS             579..>1808
                     /locus_tag="PK683_13020"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HNH09415.1"
                     /translation="MIKPLRQCLAFAGIILSLYHSSVYSQDKPADKRKLIFLSEFSPF
                     KSPPDPAVSEEIFRKFSREFESRGFEVRKASGNSIDDKLQSAKSSEAYFLVDGYYKKT
                     EGKNLNVYVQIYNPETGFVTDALPQFQEYAAFEGSEWDTEELKEFDRKLIESLGHRTA
                     VAVSSNSRKQEKRGNIENSILYSPISELQFPLSKAESGKTLSEVFKYLSEENSISIVT
                     KRVGQSESAEKTSAIVSVITRKNILDSGARNLADILKSVPGVEVFYDQFGFYKVGFRG
                     IRSQSGVLLMMDGHRMNNFYDGSVFLDIRADAIDKVEVIRGPGSSVHGTNALVGVINV
                     ITKDLPADKKSHLNVSGRYGSWNTVEPSVFYGTKIGESDWRMTGYAGQYQSERLRQTV
                     PFDDTCYQTPPAGTVFPG"
BASE COUNT          563 a          361 c          355 g          529 t
ORIGIN      
        1 aagaatcaca gcagagtccg cacatttttg tcatttcata tccgacttct ttcacgcttt
       61 cctttgtaac aaccttagta tgccggcccg atgtgataca atagcagatg acatcttctc
      121 ttgtttcgat gattctttta ttagtaactg ttaaaaaccc tctaacgcgc atacccatta
      181 tttttgctat aattctgaaa aaatttccaa caattttggt aatcggattg ttccctcttg
      241 accaaaaatc cgcttcaagt tcaaataaca tctcttctcc tggaagaaga tcaattttta
      301 ttttcatagt ttatctccta atgtcataaa aaaaatatga tctatttatg tcaatatata
      361 agactaatca catacgaata actacgaaaa agacaaattt ttcagatagg aatctctaaa
      421 aactttcttt agcaaagtca attgaaccct tcttaggaaa tttttatcct ttcacttcca
      481 ttattagagc tcccccgata tttcctttag atctgatcca taaatgattg ttcaaagcag
      541 ggggaacaga aaatatagat atatttgccg caaccgaaat gattaaaccg ttaagacaat
      601 gcttggcatt tgcaggcatt attttgtcat tataccattc ctctgtctat tctcaggata
      661 agcctgcaga caaacgaaag ctgatctttc tttcggagtt ctcccctttt aaatcgccgc
      721 ctgaccctgc tgtatcagaa gagatattcc gaaaattttc cagggaattt gaaagcaggg
      781 gatttgaagt tcgaaaagca tcaggaaact ccatcgatga taaacttcag tcagcaaaat
      841 cctctgaagc ttattttctt gtggacggtt attataaaaa aactgaggga aaaaacctca
      901 acgtctatgt ccagatttac aatccggaaa caggttttgt aactgatgca ctccctcaat
      961 ttcaggaata tgcagcgttc gagggaagtg aatgggatac tgaggaactg aaagaatttg
     1021 acagaaagct gattgaatcc cttgggcaca ggactgcagt cgcagtatct tccaattcca
     1081 gaaaacagga gaaaagaggg aacattgaaa attcaattct gtactcaccg atttctgaac
     1141 tgcagttccc tctctcaaaa gcagaatcag gaaaaacact ttcagaagta ttcaaatact
     1201 taagtgaaga aaacagtatt tcgattgtta caaaaagggt cggtcagtcg gaatcagccg
     1261 aaaagacctc tgccatcgta tcagttatta caagaaagaa tattctggat tccggtgcaa
     1321 ggaatcttgc tgacatattg aaatcagttc cgggagtgga agtattttat gaccagttcg
     1381 gattctacaa ggtaggattc agaggaatac gttcacagtc tggagttctt ctgatgatgg
     1441 acggtcacag gatgaacaat ttttatgacg gctctgtttt tctcgacatc cgtgcagatg
     1501 cgatcgacaa ggtggaagtg atcaggggtc ccggatcttc agttcatgga acgaatgcac
     1561 tggtcggtgt aataaacgta attacaaagg atcttcctgc cgataaaaaa tcgcatttga
     1621 atgtttccgg aaggtacggt tcctggaata cagttgagcc gtctgttttt tacggaacaa
     1681 aaatcggaga atccgactgg agaatgacag gttatgcggg tcagtatcag tcagagaggc
     1741 tcaggcagac tgttccattt gatgatacct gctaccagac tcctcctgca gggacagtat
     1801 ttccggga
//
LOCUS       DAOQGJ010000863         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46322_length_1807_cov_3.534817, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000863 DAOQGJ010000000
VERSION     DAOQGJ010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46322_length_1807_cov_3.534817"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PK683_13025"
     CDS             <1..1165
                     /locus_tag="PK683_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09416.1"
                     /translation="IGIWQGFAGANSALVPENTVIFSEGGYDGQFFFLNAAYLFSPES
                     GFPPVLDSFLIRFTRIGFSFLSGLFSAVLGWHHYAVSSAVLLLGFHILSSVCLFKLLP
                     EKNRPLSILYLFSPFSANANLLLVADSIQASFFIFTVYLLEKSGINLRSRNSSLYSVT
                     AYSRRNLFPVLFGTVLLFLLIKETAVIYILIFLLISLIEGRVGEGSILALALLAYSAF
                     LVYIHFSMKSFAGTYPLSFIQLSDFPLFGFIKSIKLENLTSTKPFLREMSKFPLLILL
                     VLFLCEPLFVFRNFSAGRVMPALLILSVAPALIAEQGYWLTFDNISRFFTLSIPIYVL
                     SVPVSGERKIRLHTAAVSAVIFFLFISLMLRTVWIKSPMEFYVKQTDVPHPHR"
     gene            complement(1131..>1807)
                     /locus_tag="PK683_13030"
     CDS             complement(1131..>1807)
                     /locus_tag="PK683_13030"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HNH09417.1"
                     /translation="AIFILYYIYEKRFKALVFFAAGMGFWSVLPFLAGYEYGLAQWKN
                     WIELVLQNALKNPVFRAWKNNQSLVATLAKYFHSAADPENQYKYGMPFVSLSLDQIKI
                     LLNILTLAVLSPLFIFWKRGYSRNQLISILFILSAILSGVSWIHSFSVLIFPIFYLFH
                     SLEKNRKGAWAVYGISALIILTSGSINKSLENASLMFSLLLYISLGYYALCFYISDED
                     AEHLSA"
BASE COUNT          461 a          366 c          384 g          596 t
ORIGIN      
        1 gataggaatc tggcaggggt ttgcaggggc aaattcagca ctggttcctg aaaatacagt
       61 tattttttcg gaaggagggt atgacggaca gttcttcttt ctgaatgctg cttatctttt
      121 cagcccagag tccggctttc cgccagtttt ggattccttt ctgattagat ttacaagaat
      181 aggtttttcg tttttgagcg gtcttttttc tgctgttctg gggtggcatc actatgcagt
      241 cagttctgca gtgcttctgc tgggtttcca tattttatcc tctgtctgcc tgttcaaact
      301 tcttcctgaa aaaaacagac ctctctcaat tctttatctt ttttcacctt tttctgcaaa
      361 tgccaatctt ctgcttgttg ctgattcaat tcaggcatcg ttttttattt ttactgtgta
      421 ccttctggaa aaaagcggaa taaacctgag gagcagaaat tcttcattgt attcagtcac
      481 agcctacagc aggaggaatc tctttccggt tctgttcggg acagtgcttc tgtttctttt
      541 gattaaggag actgcagtga tttacattct gatttttctc ttaatttcac tgattgaggg
      601 cagggtgggg gaggggagca tactggcatt ggcactgctg gcatattcag catttcttgt
      661 ctatattcat ttttctatga aatcttttgc aggaacttat ccattgagtt tcattcagct
      721 ctctgacttc cctctgttcg gttttataaa gagcattaag ctggaaaatc ttacgagtac
      781 taaaccattt ctcagagaaa tgtccaagtt ccctcttctg attctgcttg ttctttttct
      841 ttgcgaacct ctgtttgtat tcaggaattt ttctgcggga agggttatgc ctgcactcct
      901 cattctttcc gttgcacctg cactgatagc agaacagggg tactggctta cttttgataa
      961 tatttccaga ttctttactt tgagcattcc gatttatgtt ctttctgtac ctgtatccgg
     1021 ggaaaggaag atcagactgc ataccgctgc agtttctgcc gttattttct ttctgtttat
     1081 ttcactgatg ctcaggacag tctggataaa atcgccgatg gagttttatg tcaagcagac
     1141 agatgttccg catcctcatc gctgatataa aagcagaggg cataataacc cagtgaaata
     1201 tataagagaa gagaaaacat cagtgaggca ttttccaggg acttgtttat gctgcccgaa
     1261 gtcagaatta taagtgcgct gattccataa accgcccaag caccttttcg atttttctcc
     1321 agagagtgaa aaagataaaa aatcgggaag atcagaacag agaaagaatg aatccagctg
     1381 actccgctga gtatggctga aagtatgaac agaatgctga tcagctggtt tctgctgtat
     1441 cctcttttcc agaatatgaa aaggggagag agaaccgcaa gagtcaatat gttcagaagg
     1501 atctttatct ggtcaaggct gagtgaaaca aaaggcattc cgtatttgta ctggttttcc
     1561 ggatcggcag ctgaatgaaa atatttggcc agagttgcta cgagactctg attgtttttc
     1621 catgcacgga aaaccgggtt ctttaatgca ttctgaagta ccagctcaat ccagtttttc
     1681 cattgagcaa gtccgtactc gtatcctgcc agaaaaggta gaactgacca gaaacccatc
     1741 cctgctgcaa aaaaaaccaa tgccttaaaa cgtttttcgt atatataata tagtataaat
     1801 atggcgg
//
LOCUS       DAOQGJ010000864         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46458_length_1804_cov_3.218411, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000864 DAOQGJ010000000
VERSION     DAOQGJ010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46458_length_1804_cov_3.218411"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PK683_13035"
     CDS             complement(<1..499)
                     /locus_tag="PK683_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNH09418.1"
                     /translation="MKKNLSDLIAKYDVAAPRYTSYPTVPYWSDSPTVDEWMDSIRTS
                     LKNPSAKIALYVHLPFCETLCTFCGCNNSITKNHTVEEPYLETLFKEIDIYRKNVEQF
                     SVNEVAELHFGGGSPTYLSEKNLDTLVTKLKSSFRFSPNADLSVEVDPRRTRTTQLEV
                     LYSHGF"
     gene            complement(496..1518)
                     /locus_tag="PK683_13040"
     CDS             complement(496..1518)
                     /locus_tag="PK683_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNH09419.1"
                     /translation="MANIRFQNSLKNIPQNIPPVWMMRQAGRYHSHYRALREKYSFME
                     LCKLPEIAAEVALGPIQEFDFDVAILFSDLLFPLEALGFGLEYTDAGPKLGMQLNEEN
                     INGLKKTEDALPSLLFQKEAVRLTREKIPREKSLIGFVGGHWTLFAYAVAGRHEGGLL
                     EAKKNLPLEKKFRNIILPLLKENIRLQLDGGAEIVMIFDTSAGELDLESFREIILPGV
                     QELASAFPERIGYYSRGTSESHIKSAAKITELAGLGFDHRIDMTSMLSEKKKGFMQGN
                     FDQSFLFQEKDVFKSTVQKYFSKLKDLSPEQRKGWVCGLGHGVLPKTPEYNVKYFVQT
                     ARETFS"
     gene            complement(1505..>1804)
                     /locus_tag="PK683_13045"
     CDS             complement(1505..>1804)
                     /locus_tag="PK683_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNH09420.1"
                     /translation="GNCRISFERYEIETKDGKIISLQTRDCELLKFLIQEKHRVVSRS
                     EILDRVWGEEKFPTERTIDNSIVRLRKCFGKDGHKAIQSVRGIGYRFTGVLDGKH"
BASE COUNT          493 a          412 c          363 g          536 t
ORIGIN      
        1 tgaatccatg agagtaaaga acttccagct gagttgtcct ggttcttctc ggatccactt
       61 caacagagag atctgcattt ggggaaaaac gaaatgaact cttgagtttt gtgacaagtg
      121 tatccagatt cttttcggaa agataggtag gacttccccc gccaaaatgc agttcagcca
      181 cttcattcac agaaaactgt tctacattct ttctgtagat gtctatttcc ttgaaaagag
      241 tttccagata gggttcttcc actgtatgat ttttagtaat ggaattattg catccgcaga
      301 atgtgcagag ggtctcgcag aaaggcagat gcacatagag agcaatttta gcagaaggat
      361 ttttcagtga tgttctgatg gaatccatcc attcatccac agtcggagaa tctgaccagt
      421 agggaactgt agggtaactg gtatatctcg gtgcggcgac gtcgtacttg gcgattagat
      481 cagaaagatt ttttttcatg aaaaagtctc ccgtgctgtc tgcacaaaat attttacatt
      541 gtattcagga gtctttggaa gaacgccgtg cccgagtccg catacccaac cttttctctg
      601 ttcaggactt aagtccttca gtttcgaaaa gtacttctgc actgtggatt taaaaacatc
      661 cttttcctga aaaagaaatg actgatcaaa attcccctgc ataaatcctt tctttttttc
      721 ggaaagcatt gaagtcatat caattctatg gtcaaaaccg agaccagcca gttcagtgat
      781 cttcgcagca gattttatat gcgattcgct ggttccgcga gaatagtatc cgatcctttc
      841 aggaaatgca gatgcaagct cctgaacgcc gggcagaatg atttctctga aagattccag
      901 atccagttca cctgcagatg tatcaaatat cattacaatc tcagcaccac cgtccagctg
      961 aagacgtata ttctccttca gaagaggaag aatgatattc ctaaatttct tttcgagagg
     1021 aagattcttt tttgcttcga gcagtccgcc ctcatgtctc cctgccacag catatgcaaa
     1081 cagtgtccag tgaccgccta caaatcctat cagagacttt tcccggggaa ttttttcacg
     1141 cgtcagtctg actgcttcct tctgaaacag aagcgaagga agagcatcct cggtcttttt
     1201 aagcccgtta atgttttctt cattcagctg cattccgagt ttaggaccgg catctgtgta
     1261 ttccagaccg aaaccaagcg cctccagagg gaaaagaaga tctgaaaaaa gaatagccac
     1321 atcaaaatca aattcctgga ttggacccag tgccacttca gcggcgattt cagggagctt
     1381 gcagagttcc ataaaactgt atttttcccg aagtgccctg taatgagaat gatatcgtcc
     1441 tgcctgtctc atcatccata caggcggaat attctgcgga atatttttca atgaattttg
     1501 aaatctaatg tttgccatct aagactcctg tgaaacggta accgatccct cttaccgatt
     1561 gaattgcttt atgaccatct tttccaaagc attttctaag gcgaacaatc gaattgtcta
     1621 ttgtgcgctc tgtaggaaat ttttcttccc cccagaccct gtccaatatt tcggaacggg
     1681 agacgaccct gtgtttttcc tgaatcagaa atttcagaag ttcacagtcc ctggtctgaa
     1741 gagaaataat cttcccgtcc tttgtctcaa tttcgtatct ttcgaaactg attctgcagt
     1801 tccc
//
LOCUS       DAOQGJ010000865         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46493_length_1803_cov_2.594966, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000865 DAOQGJ010000000
VERSION     DAOQGJ010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46493_length_1803_cov_2.594966"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1803
                     /locus_tag="PK683_13050"
     CDS             <1..>1803
                     /locus_tag="PK683_13050"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNH09421.1"
                     /translation="KLKDANGNVIATTTTNASGQYSFSSLLSSGVYSIEVTKNGFMTS
                     NSSFSFTPNPSGSATNVTQSGGPNKMVPKPSNVTGQVSGPSGTPPRIEGASINFRPDS
                     TQKPSNLLYLATGTDDRLRNLASLWMREACLAVSACATACSAQSFAPSCAAANQGAGP
                     SWTYTTYANKVYETSGNNVLFTAVAGVWSYYVAAPGYQNSSVMSITLNGQDVNAAPVV
                     LNPTTHRGQIAGQTVVLDTLVAGTKNAYGAPVSGYTTQTGVPGMFSVLLGNSDNSGNP
                     VAHITVTNASGQYAFDGNSKVVALPPLTTLCSISALVSSVAPGNTSLSGQSCNDNADA
                     LRVAYAISQYSTTAKLLSDASNNVIGGDAASVCTTGCPVTATGYQFRAGSYNIIVADP
                     LKHMSASSTGVNISTSNPATSGSLVSVVNSVLHLPRRQITGTISDAISTGPMSGATVE
                     LGVDTDSDPSTITWGPARRDPDTLSGSRLAASDIQVPSVTTDANGQYAINNVDPGTYV
                     LRVSRPGYVTELVQVTVPSTGSATVVNLGIVQDGPRGNIAGRVVLAGGAPFTGTYTLE
                     IIHPTAGTRPTSPVAPSSLTSGTSVFSNVPNYSI"
BASE COUNT          487 a          431 c          408 g          477 t
ORIGIN      
        1 aaaactgaaa gatgcaaatg gaaatgtaat tgcaacaaca actacaaatg cgagcggtca
       61 gtattctttc agcagccttc tcagttcagg agtctattcc atagaagtga ctaaaaacgg
      121 atttatgact tctaacagtt cattttcttt cacacctaat cccagcggat cagctacaaa
      181 tgtgactcag tccggcggac caaacaagat ggttcctaaa ccttcaaatg tgactggcca
      241 agtgtcagga ccctccggaa cacctcctag aatagaagga gcttccatca atttccggcc
      301 cgacagtacg caaaaaccat ccaacctgct gtatctggca acaggtaccg atgacaggct
      361 cagaaacctt gcatcactct ggatgagaga ggcatgtctg gcagtatcag cctgtgcgac
      421 agcatgttct gcacagtcat ttgctccttc ctgcgctgcc gcaaatcagg gagcaggtcc
      481 gtcatggact tatactacat atgccaataa ggtttatgaa accagcggaa acaatgtgtt
      541 atttacagca gttgcaggtg tttggagcta ttatgttgct gccccaggat accaaaattc
      601 aagtgtaatg tctattaccc tgaacggcca ggatgtcaat gctgcccctg ttgtactgaa
      661 tccgacgact cacagaggac agattgccgg ccagactgtg gttctcgata cattggttgc
      721 aggtacaaaa aatgcttacg gtgcgcctgt gtccggttac actacacaga ctggtgtccc
      781 tggaatgttt tctgttcttt taggaaactc ggataattct ggaaatccgg tggcacatat
      841 taccgttaca aatgcttcag ggcagtatgc atttgatgga aattcgaaag tagtggctct
      901 tcctccgctg acaacactct gcagcatcag tgctcttgta tccagcgttg cccctggaaa
      961 cacttcactt tcaggtcagt cctgcaatga caatgcggat gcattgagag ttgcatatgc
     1021 gatcagtcaa tacagtacaa ctgcaaaact tttaagcgat gcatccaata atgtaatcgg
     1081 cggagatgct gcttcagtct gcacgacagg atgtcctgtg actgcgacag gttatcagtt
     1141 ccgtgcaggt tcttacaaca tcatcgttgc tgatcctttg aagcatatga gtgcttcttc
     1201 tactggggtc aatatctcta catccaatcc tgccacatca ggttcacttg tatctgtagt
     1261 aaattcggtt ctgcatcttc ctagaagaca gattacagga acaatcagtg atgccatctc
     1321 gactggtccg atgagcggtg ccacagttga gcttggagtg gatacagatt ccgatccttc
     1381 aacaatcaca tggggaccgg ccagaagaga tcctgacact ctttccggat caagacttgc
     1441 tgcatccgat atccaagtac cttcagttac aacagacgcg aacggtcagt atgcaattaa
     1501 caatgtggat ccggggactt atgttctaag agtctcgaga ccaggatatg tgactgaact
     1561 cgttcaggtg actgttccaa gcactggatc agcaactgta gtcaatttgg gaattgttca
     1621 ggatggtcct agaggtaata ttgcaggtag agttgttctg gccggcggtg ctccttttac
     1681 aggaacgtat acactggaaa ttattcaccc taccgcagga acaagaccta cttctcctgt
     1741 ggctccatcg agtctgacaa gcggaacatc agtgttttcc aacgttccaa attacagtat
     1801 att
//
LOCUS       DAOQGJ010000866         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46533_length_1802_cov_2.834574, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000866 DAOQGJ010000000
VERSION     DAOQGJ010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46533_length_1802_cov_2.834574"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PK683_13055"
     CDS             <1..325
                     /locus_tag="PK683_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09422.1"
                     /translation="VMQDKSPPRIGFPYLLYRDYYLPDLKTPEMTEKFYEISDLGSGI
                     APGMTVLLEGAYYPYEYDTDRSFIRIEIPNSFSRYKKNLMIQIQVKDKAGNKSDWFTD
                     FVKLK"
     gene            322..>1802
                     /locus_tag="PK683_13060"
     CDS             322..>1802
                     /locus_tag="PK683_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH09423.1"
                     /translation="MIGLRNKLIIGFIVLDIFLSIVLGLTMYTYSIDMFISDFRNHKM
                     SIARFIANSIKGEEHRRFSSFQSVKDTVFLSYYREMSNILRQEATARYIYTLNYDRET
                     EALTYGIDANPSYADTVWAESEAVSFYFIYSGEKFIIDHDKSRVTDSKEIIIGGISNI
                     LRINQDTGDVFLNDKKIFGVINRNPLQISTESGRLDRENRKRSVRVKINSDPVEFTLT
                     FTEKDMPGTDPGAPFIEKEAVKNKIRRLIRTASEYIDEEPVQNAYGYFLSSYAVLKDS
                     AGNGIGIVIVDIDSQEISEFKNKFTIVAISISTFTFVLTIVLSLLLAKYFTLPLEVLT
                     KAVDDLASGNMETIVDIKSKDEFGKLAKSFNAMVNNLKIASEVQFNLITEISQLNDSL
                     EKKVQARTKTIQAQSQELEKQILIAKKIQIALLPEKIPKIDNASVSFKYEPMMGVGGD
                     FLDFYYRNENELNLFICDVSGHGVPAAFLASMVKMSLQECYER"
BASE COUNT          598 a          345 c          390 g          469 t
ORIGIN      
        1 cgtaatgcag gacaagtctc ctcccagaat cggattcccc tatcttctgt acagggatta
       61 ttaccttcct gatctgaaga cgcctgaaat gacagagaaa ttttatgaaa tcagtgatct
      121 gggctcaggg atcgcaccgg ggatgaccgt tcttctggaa ggtgcatatt atccgtatga
      181 atatgacact gacagaagtt tcatcagaat tgaaattccg aattcatttt ccaggtacaa
      241 aaaaaatctg atgattcaga ttcaggtgaa agacaaagca ggcaacaagt ctgactggtt
      301 tacggacttt gtaaaattga aatgatcggt cttcgcaata agctgatcat agggttcata
      361 gtactggaca ttttcctgtc catcgtgctg gggcttacta tgtatacata ttcaattgat
      421 atgttcatca gtgatttcag aaatcacaaa atgtccatag caaggtttat tgcgaattcc
      481 atcaaaggcg aagaacacag gagattcagc agttttcagt cagtcaagga tactgtgttt
      541 ctctcttatt acagagaaat gtcgaacata ctcagacagg aagctacagc aagatacatc
      601 tacaccctga actacgacag ggaaacagaa gcactgacgt acggaataga tgcaaatccc
      661 tcctatgcag atacggtatg ggcggaatcg gaggcagtca gtttttattt catttattca
      721 ggtgaaaaat tcattataga tcatgataaa tccagagtga ctgattcaaa ggaaatcatc
      781 attggaggaa tctcaaacat actgagaatc aatcaggata caggagatgt atttcttaat
      841 gataaaaaaa tattcggagt gataaacagg aaccctcttc agatcagcac cgaatccgga
      901 agactggaca gggaaaacag aaaaaggtct gtcagagtga aaattaactc tgaccccgtt
      961 gaattcactc tgacattcac tgaaaaggat atgccaggga ctgatccggg agcgccgttc
     1021 attgaaaagg aagctgtaaa aaacaaaatc agacgcctta tccggactgc ctcggaatat
     1081 atagatgaag agcctgtaca gaatgcttac ggatacttct taagctctta tgcagttttg
     1141 aaggacagtg caggaaacgg aataggcata gtgattgtgg acattgacag tcaggaaatc
     1201 agcgagttca agaataagtt tacaatcgtg gccatcagca tatccacatt tacctttgtg
     1261 ctgacaattg tactttccct cctgctggca aaatacttta cccttcctct cgaagtgctg
     1321 acgaaagctg ttgatgatct tgcatcagga aatatggaaa caattgtaga catcaagtcc
     1381 aaggatgaat ttggaaaact tgccaaaagt ttcaatgcaa tggtaaacaa tctgaaaatt
     1441 gcctccgagg tgcagttcaa tctgatcact gagatcagtc agctgaatga cagtctcgaa
     1501 aagaaagtgc aggcaagaac caagacaata caggcacagt ctcaggaact tgaaaagcag
     1561 attctgattg caaaaaaaat acagattgca ctccttcctg aaaaaattcc caaaatagac
     1621 aatgcaagcg tcagtttcaa gtatgaacct atgatgggag tgggagggga ttttctcgat
     1681 ttttattaca gaaatgaaaa tgaactgaat cttttcatat gcgatgtttc ggggcacgga
     1741 gttcctgcag cattccttgc atccatggta aagatgtctc ttcaggaatg ttatgaaaga
     1801 ag
//
LOCUS       DAOQGJ010000867         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46584_length_1801_cov_2.585911, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000867 DAOQGJ010000000
VERSION     DAOQGJ010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46584_length_1801_cov_2.585911"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1237
                     /locus_tag="PK683_13065"
     CDS             23..1237
                     /locus_tag="PK683_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH09424.1"
                     /translation="MSRTVVNYPKGAHIVMEGSENPGFIYILKSGEILIESKIQFIHK
                     KLNRYYPGDCFGFVSGIMKKKHQYNLVALNECEVIRLTIDSFVEFMVNNKDTFLHFLS
                     SNSERLRIFLNNIDQSREYGYEEIPPEELIHDARLYKRLGRKDLSCYALARYLEKNFE
                     QPKNPVKMEEAKDMLNAMDKNYKFPVVEQFADESGLIYHQGSIIFVENEPPDYFYLIK
                     SGRVGIHKLVDEKLICLEVIGKGEIFGEMGILNSKGRIASAIAFEDCVLQSYDRRDIF
                     EIRDAEILDKIFRMIAKRMWLASNRFYLRQIADIDIQMYLLLQILVEERLLKPKKDDT
                     AEVDIPLSIYDLGKMIGKDETDIKKMNELNLDRNIKIQPNKITVLDKNKFLEKAEILQ
                     KKHARQMKEVII"
     gene            1239..>1801
                     /locus_tag="PK683_13070"
     CDS             1239..>1801
                     /locus_tag="PK683_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09425.1"
                     /translation="MHQVLLVDSNYKDLEQVEYYLRGIRLKIVSTSNVKKIMEFARSA
                     PPDLMIIGLEPTDSEMLDKIKLLRFDSITRHSLILAIFKTEDKKQLNKFHTENVHAVM
                     IHPVFKLELREMVDKLLSTAQEKRTKHEQETKDYIMIDRSQEGQTHIILEGGLRRNVG
                     PQLRTFFTKEFIDEAKKTVVCLDLRLI"
BASE COUNT          617 a          332 c          374 g          478 t
ORIGIN      
        1 ggccaatcag gactgattga aaatgtccag aaccgttgta aattatccta aaggggccca
       61 tattgtaatg gagggctcgg agaatcccgg atttatttac atactgaaat ctggagaaat
      121 actgattgaa tccaaaattc agttcatcca caaaaaactg aacaggtatt atcccggcga
      181 ctgcttcggc tttgtatccg ggataatgaa aaaaaagcac cagtacaatc tcgttgcact
      241 taatgaatgc gaagtcattc gcctcacaat agattctttt gttgaattta tggtgaacaa
      301 taaagacact ttcctgcatt ttttatcctc caattcagaa cgtctcagaa tatttctgaa
      361 taatatagat cagtcgcgtg aatacggtta cgaggagatc cctccggaag aactgatcca
      421 tgatgcacgc ctttataaac ggcttggcag aaaggacctt tcctgctatg ctttggccag
      481 gtatctggaa aagaattttg agcagccgaa aaatccagta aaaatggaag aagccaagga
      541 tatgctcaat gcaatggaca agaattataa atttccagtc gtggagcagt tcgcagacga
      601 gtcaggctta atttatcatc agggttccat catttttgtt gaaaatgaac cccctgacta
      661 tttttatctg atcaaaagcg gtcgagtggg aattcacaag cttgtggatg aaaaactgat
      721 ctgtctggaa gtcattggaa aaggtgaaat attcggtgaa atgggaatat tgaacagcaa
      781 gggacgtatc gcttctgcaa ttgcctttga agactgcgta ttgcagagtt atgacagaag
      841 agacatattt gaaatcaggg atgctgaaat tctggataag attttcagaa tgattgcaaa
      901 aagaatgtgg ctggccagca acaggtttta tctcagacag attgccgata tagacattca
      961 gatgtacctg ctccttcaaa ttcttgtaga agaacgtctt ctgaagccta aaaaggatga
     1021 taccgcagaa gtggatattc ctctttcaat ttatgatctg ggaaaaatga taggcaagga
     1081 tgaaacagac atcaaaaaga tgaacgaact gaacctggac agaaatataa aaatccagcc
     1141 gaataaaatt actgtccttg acaaaaataa atttttagaa aaagctgaaa tacttcaaaa
     1201 aaaacatgct cgccaaatga aagaggttat tatctgatat gcaccaagtg ctgctggttg
     1261 actcaaacta taaagatctc gagcaggtag agtattatct tcggggcatt cgactgaaaa
     1321 tagtaagcac gagtaatgta aaaaaaatta tggaatttgc cagatccgct cctcccgatc
     1381 ttatgattat agggcttgag ccgactgatt ccgaaatgct cgataaaatc aaactcctgc
     1441 gttttgattc catcacaaga cactctctca ttcttgccat atttaaaact gaggataaaa
     1501 aacagttaaa taaatttcat acagaaaatg ttcatgcagt aatgattcat ccagtcttta
     1561 agctcgaact ccgtgaaatg gtggataagc ttttaagcac tgctcaggaa aaaaggacaa
     1621 aacatgagca ggaaacaaaa gactacatta tgatcgacag gagtcaggaa ggccagactc
     1681 atatcattct ggaaggagga ctcagaagaa atgtcggtcc tcagctcaga actttcttta
     1741 ctaaagaatt catcgatgag gcaaagaaaa cagtagtctg tctggatctc cgtctgattc
     1801 a
//
LOCUS       DAOQGJ010000868         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46626_length_1800_cov_2.773639, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000868 DAOQGJ010000000
VERSION     DAOQGJ010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46626_length_1800_cov_2.773639"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK683_13075"
     CDS             complement(<1..312)
                     /locus_tag="PK683_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH09426.1"
                     /translation="MNISSIQLNDKLKKREKGEENFLLLDVRNRTEQDICTIPGTDIL
                     IPVKELNIELHKLNDWKDKEIVVYCKSGIRSKTAEGILKENGFQKITHLNKGILEYIE
                     TV"
     gene            complement(332..508)
                     /locus_tag="PK683_13080"
     CDS             complement(332..508)
                     /locus_tag="PK683_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HNH09427.1"
                     /translation="MKFADPKNDIAFRKIFGNETKKIILISFLNSVLNLEDDKNTISS
                     STDLSLEEIENLQI"
     gene            complement(628..1422)
                     /locus_tag="PK683_13085"
     CDS             complement(628..1422)
                     /locus_tag="PK683_13085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNH09428.1"
                     /translation="MKLSILIVSIFFSANMLSAQTLTPKVLKGTGSFYADQFNGRRTS
                     SGEIFDMMKFTAAHREFPFNTILKVTNTATGEYVLVRVNDRGPFIMDRIIDLSYAAAD
                     KLGYVPIGLQEVELEFLGIGKPEENPPQAIEDLEAYKKTLSQGLPEDYHQLYPTDPAK
                     LIMDARAKKTYRDLQGNTVRTPKGFGLQIAVYANEDYAKTLGADVASKGFSNVYIESF
                     YNSKNGVTYYRVIYGEFETMQMTAPESKKLLQAGFDAHFIYTYPKD"
     gene            complement(1419..>1800)
                     /locus_tag="PK683_13090"
     CDS             complement(1419..>1800)
                     /locus_tag="PK683_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09429.1"
                     /translation="EKAAQTVENAENQEEKGAKKASTRTYKPRKKKVEDAFDVVDKLG
                     NTGAKLPDIQRIPPPKDQGFHIQFKLDLISVEDILNPEKNADNKKDSSIVFDFTIPYI
                     WNLPVFNEITKTVVNELKRLKVLQ"
BASE COUNT          478 a          377 c          330 g          615 t
ORIGIN      
        1 caccgtctct atgtattcca aaataccttt gttcagatgg gtaattttct gaaatccgtt
       61 ttccttcaga atgccctccg cggtctttga tctgattcct gacttgcagt agactacaat
      121 ttccttatcc ttccagtcat tcagtttatg cagttcaata ttcagttcct ttaccgggat
      181 aagaatatca gttccgggta tggtgcagat atcctgttcc gttctgtttc ggacatccag
      241 aagaagaaaa ttttcttctc ccttttctct tttctttaat ttatcattaa gttgtataga
      301 tgatatattc atattcccgt ccaggcatta tttaaatctg caggttttca atctcttcca
      361 gactgagatc ggttgaactg gaaatcgtat tcttatcatc ttccagattc agcactgagt
      421 tcaaaaaaga gataagaata atctttttag tttcatttcc aaaaatcttg cggaaagcaa
      481 tatcgttttt cggatcagcg aatttcataa gtatagtttc ctctgtccaa ataaaatgtc
      541 aagaggagta atttggatat tatttatgtt ttattatttt gaaaacaaga catcgaggtt
      601 ttcaaaaacc tcgatgttta ttcacgttca gtctttagga tatgtgtaaa tgaaatgcgc
      661 gtcgaagcct gcctgcagaa gtttcttcga ttcaggcgct gtcatctgca ttgtctcaaa
      721 ttcaccgtag atgaccctat aataagtgac accgttttta ctgttgtaaa atgattcaat
      781 ataaacattg gagaagccct tggaggcgac atcagctcca agtgtttttg catagtcctc
      841 atttgcatag acagcaatct gaagaccgaa tcctttggga gttctgactg tatttccctg
      901 aagatctctg taagtctttt tggctcttgc atccataatc agctttgcag gatcagtagg
      961 atacagctga tgataatcct caggcagtcc ctgagaaaga gtctttttat aggcttccag
     1021 atcttctatt gcctgaggag gattttcttc cggcttgcct attcccagaa actccagctc
     1081 cacttcctgc agtccgatag gaacgtatcc cagcttgtct gcagcggcgt aagagaggtc
     1141 aatgatacga tccattatga atggacctct gtcattgact ctgactaaaa cgtactcacc
     1201 agtagcagta tttgtcactt tcagaattgt attgaaagga aattctctgt gagccgccgt
     1261 aaatttcatc atatcaaata tctcaccgga agaagttctt cttccattga actgatctgc
     1321 ataaaaagaa ccggttcctt tcagaacttt gggagtcaaa gtctgagcag agagcatatt
     1381 tgcagaaaag aaaattgata ctataagaat ggacagtttc attgcagcac cttcagtctt
     1441 ttaagttcat tgacgacagt tttggtgatt tcattgaaaa ccggcaggtt ccaaatatac
     1501 ggaatcgtaa aatcaaaaac aatactgctg tcttttttat tatctgcatt tttttccgga
     1561 ttcagtatat cttctacgct gatgaggtcc aatttaaact gaatatggaa tccctgatcc
     1621 ttaggaggcg gaatcctctg gatatcagga agttttgccc ctgtatttcc cagtttgtcc
     1681 accacatcaa aagcatcttc cactttcttt tttctgggtt tgtaagtcct ggttgaagct
     1741 ttctttgctc ccttttcttc ctgattttcc gcattttcga cagtttgagc tgccttttcc
//
LOCUS       DAOQGJ010000869         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46632_length_1800_cov_2.299713, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000869 DAOQGJ010000000
VERSION     DAOQGJ010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46632_length_1800_cov_2.299713"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1800
                     /locus_tag="PK683_13095"
     CDS             <1..>1800
                     /locus_tag="PK683_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH09430.1"
                     /translation="SENLHIITKQNEILEKMVQQRTEELLQQNAKLKSAEEKLNQNIK
                     ELTDAQETVAVTLAQNEAVTAALNNSTIVSIADLKGKIIKVNQMFCAVSGYKEEELLG
                     KNHRIVNSGFHSREFWSDMWRTVTSGKVWRSEVCNRAKNGELYWVDTVINPVYDKNDK
                     IIQYISIRSLMTERKKAEQELLSQKQYTDSLLASIPDLVFVLDKDGRFLEYRAGRSSD
                     LSVPVEAFLHKNISEVFPPDFVISVKDCIQKAFSGDTSVSFEYQMPVNGKIGDYEARF
                     APIGHEKIIVLVQNISERKQAEKKLIESETKLRGILDSTTEINILISPDLKILSFNKI
                     AGQKIELIYGTPPKLYEDFRKYILNGLEESFYGHFSKALNGEYVVLERELIFSKTRNW
                     FEFRYNPVFDSMGKVIAVSMNMTDINKRKQAEITLKQKIEMLDIINTLQHSFIADSRS
                     FEVFEKILQALLDITESEHGFIGEILHTDENVPYLKIFAVTNIRWNEANEKFYNENLT
                     NGLELYNLNSIFGAVITGQKTIISNSPGTHPERAGLPEGHPPLNTFLGLPLFAGNKMI
                     GMAGVSNRPGGYDYALVETLGPLLTTIGRLIDAA"
BASE COUNT          595 a          323 c          418 g          464 t
ORIGIN      
        1 gagtgaaaat ctgcatatca ttacaaagca gaatgaaata ctcgaaaaaa tggttcaaca
       61 gagaacagag gaacttctgc agcagaatgc aaagctgaaa tctgcagaag agaaactgaa
      121 tcagaacata aaggaactca ctgatgctca ggaaactgta gcggtgactc ttgcgcagaa
      181 tgaggcagtg actgcagctc tgaacaacag cacgatcgtt tcgattgccg atcttaaggg
      241 taaaatcatt aaagtaaatc aaatgttctg cgcagtatcc ggttataaag aagaggaact
      301 gctcgggaaa aatcacagga tagtcaattc aggatttcat tccagggaat tctggtcaga
      361 tatgtggagg actgtcactt caggaaaagt gtggaggtcg gaggtatgca accgtgcaaa
      421 gaacggagaa ctttactggg tggatactgt catcaatcct gtatacgata aaaatgataa
      481 aataattcaa tatatttcaa tacgcagtct gatgacggag agaaagaaag cagagcagga
      541 gctcctttca cagaagcagt atacggattc actgctcgca tcaattcccg atcttgtctt
      601 tgttctggat aaggatggaa gatttctgga gtacagggca ggaagaagtt ctgatctttc
      661 tgttcctgtg gaagcgtttt tgcataagaa catatcggaa gtctttcctc cggattttgt
      721 gatatcagta aaagactgca ttcaaaaggc gttttcggga gatacctctg taagttttga
      781 ataccagatg cctgtgaacg gtaaaatagg ggactatgaa gcccgttttg caccgattgg
      841 tcatgaaaaa ataattgttc ttgttcagaa tatttctgaa cggaagcagg ctgaaaagaa
      901 actgattgaa tcagagacaa aactcagagg aatcttggac agcacaactg aaatcaacat
      961 tctgatcagt cctgatctta aaattctttc attcaataaa atagccggac agaaaataga
     1021 gctgatctac ggaacgccgc cgaagcttta tgaggatttc agaaagtata ttcttaatgg
     1081 gctggaagag agcttttacg gtcatttcag taaggctttg aacggagaat atgtggttct
     1141 ggaaagggag ctgattttca gcaagacacg gaactggttt gagttcagat ataatccagt
     1201 ttttgacagt atgggtaaag tcatagcagt cagtatgaat atgactgata tcaataaacg
     1261 caagcaggca gagatcacac tgaaacagaa aatagaaatg ctggatatca tcaatacgct
     1321 tcagcattcc tttattgcag acagcagatc gtttgaagtt tttgaaaaaa tacttcaggc
     1381 gcttctggac attacagaga gcgagcacgg atttatcgga gaaattctgc acactgacga
     1441 aaatgttccc tacctcaaaa tatttgcggt tacaaatatc agatggaatg aagcaaatga
     1501 gaaattctac aatgagaatt tgactaatgg tctggaactc tacaatttaa acagcatttt
     1561 cggtgcggta attactgggc aaaaaacaat aatctccaat tcaccgggca ctcatccaga
     1621 aagggccgga cttcctgaag gccatccccc attgaacact tttcttggac tgcctctctt
     1681 tgctggaaat aaaatgatag gtatggcagg tgtttcaaac agaccaggag gttatgatta
     1741 tgctctggtt gagacgctgg ggcctcttct gacgacaata ggaagactga tagatgcagc
//
LOCUS       DAOQGJ010000870         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46687_length_1798_cov_4.088927, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000870 DAOQGJ010000000
VERSION     DAOQGJ010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46687_length_1798_cov_4.088927"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK683_13100"
     CDS             complement(<1..366)
                     /locus_tag="PK683_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter, Ompp1/FadL/TodX family protein"
                     /protein_id="HNH09431.1"
                     /translation="MLRFILAAVFLLLTSVLQSDAYHNIPGFFGERAAGLGGAYTAVS
                     DDVAGAFYNPGGLAFVQNTYYSVNASSYRTISEKHSNLFGPGQNYKRVSRNYMPNFIG
                     FVRNVGKLSFGFTLANPTNE"
     gene            complement(392..1714)
                     /locus_tag="PK683_13105"
     CDS             complement(392..1714)
                     /locus_tag="PK683_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09432.1"
                     /translation="MKKLDSSFNMTKLIPAVPLPLRGTLSSVRSGLGDVLTLNGNLDF
                     QNRAGQKKISMDLGGSFPAIELRDLKIDLALNLLNDAAKTVAIDRFLLTGFGRKLRAD
                     FKGKFFKPFNKSAPFGEYTGDMKGNLTLESKTPERVLKGITFAGDIDLDFALKGPIAQ
                     GRLFSKDSYLKIVSQNCPDGKCNFYELKSIKMDIPFIHDISDKTTENLIKGNKENFIQ
                     NYGQTLPANLSIGTVTGTHPFLPDSTLDFVKPTEMYPGFSARVDYKENVLTVDNLRLF
                     TLDGLIFGKDILFNVGSGNTEKIQYAAVLQVRDVDLKQLLKKEARDKVDDGKIKADLN
                     ISGENLKDPISNVSLFFSVFQIGKDFSKSAVNIVKSTDKITDFFINSYSVDKIEVELN
                     RGLVYAKILFKKSVLNSIVSIEDDRIQQERFPLSNFLNRAKSELSVYQ"
BASE COUNT          528 a          402 c          334 g          534 t
ORIGIN      
        1 ttcattggtc gggtttgcca aagtgaaacc gaaactgagt tttcctacat tccgtacaaa
       61 accgataaag ttcggcatat aatttctgga aacacgctta tagttctgac caggaccgaa
      121 aagattggaa tgcttttcgc tgattgttct gtagctgctg gcattgacgg aataatatgt
      181 gttctgcacg aatgcaagcc cgcccggatt gtaaaatgca ccggcaacat catccgaaac
      241 tgcagtgtat gctcccccca gtccggcagc tctttctcca aaaaaacccg gaatattatg
      301 ataggcatcc gactgcaata cactggttaa aagtaaaaat actgcggcta atataaaacg
      361 taacatatta tctcccgatt tattgttgaa attactgata tactgaaagc tctgattttg
      421 ctctgttaag aaagttggaa agaggaaatc tttcctgctg aatccgatca tcttcaatgg
      481 acacaattga gttgagaaca gatttcttga acagaatttt tgcatataca agacctctgt
      541 tcagctcaac ttctatcttg tccacactgt aactgttaat gaaaaaatct gtaattttat
      601 ccgttgactt cacgatgttc actgcactct ttgaaaaatc cttcccaatc tgaaatacac
      661 tgaaaaacag acttacattg gaaataggat ccttcagatt ctcaccgctg atattcaagt
      721 ccgctttgat ctttccgtca tccactttat ctctcgcttc ctttttcaga agctgcttta
      781 aatccacatc cctgacctga agaacggctg catactgtat cttttcagta tttccgcttc
      841 ctacattgaa aagaatatcc tttccgaaaa taagtccatc cagagtaaaa agcctgagat
      901 tgtctacggt cagaacattt tccttgtaat caacccttgc tgaaaagccg ggatacattt
      961 cagtcggctt cacaaaatcc agagtggaat caggaagaaa cggatgagtt cctgtaactg
     1021 tacctatgct gaggtttgca ggaagagtct gaccgtaatt ctgtatgaag ttttccttgt
     1081 ttcccttgat cagattctcg gtagttttgt cggaaatatc gtgtataaaa ggtatatcca
     1141 ttttaatgct ttttaattca taaaaattac acttcccgtc aggacagttc tgtgatacaa
     1201 tcttcagata ggaatccttt gaaaacagtc ttccctgtgc aataggccct ttcagcgcaa
     1261 aatcaagatc aatgtcccct gcaaatgtaa tgcctttcag cacacgctca ggagttttgg
     1321 actcaagagt cagattgccc ttcatatctc ctgtgtattc tccgaaaggt gcagacttgt
     1381 tgaaaggctt gaaaaacttt cccttaaaat ctgccctcag ctttcttccg aatccagtca
     1441 gaagaaatct gtcaattgcg acagtcttgg cagcatcgtt cagaagattc agtgccagat
     1501 caatcttaag atcccttagt tcaattgcag gaaaacttcc tcccaaatcc attgaaatct
     1561 ttttctgtcc cgccctgttc tgaaaatcca ggttcccgtt caaggtaaga acatctccaa
     1621 gtccggacct gacactcgat agagttcctc gcagaggaag cgggactgca ggaatcagtt
     1681 tagtcatatt gaagctggag tcaagtttct tcacctgaag cagaagacat ctgtttaaaa
     1741 tcgggcagga aaagtctgga tgaaaaaatt cgttcctttg aagaagaaat tgcaaata
//
LOCUS       DAOQGJ010000871         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46746_length_1797_cov_2.979334, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000871 DAOQGJ010000000
VERSION     DAOQGJ010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46746_length_1797_cov_2.979334"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1031
                     /locus_tag="PK683_13110"
     CDS             135..1031
                     /locus_tag="PK683_13110"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNH09433.1"
                     /translation="MKLFIPGAAVLIFHCSLIQSSKDNNKNTLYLAAAYLASKSSSSC
                     TTNACKTTCYGSTIKTTSIDSSMPSWISNNFACVKAYKSTESGVDYYVFETEDVPPHK
                     SPYWGTSSAYYEAMPSGNTQLISSFTAQSYKFKIPVTPTVASGGSVSVPGVAAGVAVN
                     GVVLFFGQASPGNSLSSELSTFDSAQGHPDGGSKRYHYHALPKYITNYETSLLGIVSD
                     GYPVYGSKEEDGTTPSTSAATGSTSQYPKLNANTYGHSHSTKDFPNGTFHYHITNWDS
                     SVNIPSMPIYTYGTITISNITY"
     gene            complement(1056..1250)
                     /locus_tag="PK683_13115"
     CDS             complement(1056..1250)
                     /locus_tag="PK683_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09434.1"
                     /translation="MKDKNQSALEMKFKNLLESHLESFKQFASESVLKKRKTYLTKEQ
                     LSEQILKLKESLAQMEEELK"
     gene            1486..>1797
                     /locus_tag="PK683_13120"
     CDS             1486..>1797
                     /locus_tag="PK683_13120"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH09435.1"
                     /translation="MDNLARKLFTEEEYLEKERQAANKSEYYKGEVFAMAGAKRKHNA
                     IVTALIANLYPKLKDNPCEVYPSDMKVKNKTDRFYTYPDVTIVCGKPEFLDDTEDVLT
                     NP"
BASE COUNT          541 a          391 c          351 g          514 t
ORIGIN      
        1 aaatgccaat ggtgatatag aaagaatcat tgaagatgta caaaaagcat ttcgggaaat
       61 aaataaatga tcttccggat ttttgtttac aatttctttc caaaaaacgt attgtaaaaa
      121 gtcgggagaa atttatgaaa ctgtttattc caggagcggc tgtactgata ttccattgca
      181 gcctgatcca gtcttcaaag gataataata aaaacactct ttatctggct gccgcttatc
      241 tggcatcaaa atcttcatca tcctgcacaa cgaatgcctg caagacaacc tgctatggaa
      301 gcacaatcaa aaccacttcc attgactctt caatgcctag ctggatttca aataattttg
      361 cctgcgtgaa agcgtataaa agcactgaga gcggagttga ttattatgta tttgaaacag
      421 aagatgtccc tcctcataaa agtccttact ggggaacctc cagcgcatac tatgaagcaa
      481 tgccgtccgg aaacacacag ctgatttcga gtttcactgc gcagagttac aaatttaaga
      541 ttcctgtgac acctacagtt gcttcaggcg gttcagtgtc cgtgccgggc gttgccgccg
      601 gagttgcagt caacggagtt gtgctttttt ttggacaggc cagtccagga aacagtctca
      661 gctcggaact cagcacattt gattctgcac agggacatcc cgacggagga tctaaaagat
      721 atcattatca tgctcttccg aaatatatca caaattatga gacatccctt ctgggaattg
      781 tatcggacgg gtaccctgta tacgggtcaa aggaagaaga cggaacaaca cccagcactt
      841 cagcggccac aggctccacg agtcagtatc cgaagctgaa tgcaaataca tacggacaca
      901 gccactccac gaaagatttt ccgaacggaa cttttcatta tcatattacg aactgggaca
      961 gttcagtcaa tattccgtct atgccgattt atacttatgg gacaattact ataagtaata
     1021 ttacttacta aattattatt ataaagggaa ctgaattatt tcagttcctc ttccatctga
     1081 gccagggatt ctttgagttt cagaatctgc tctgagagct gttctttagt aagatacgtc
     1141 tttctttttt tcagaacact ctcagatgca aactgcttga aactttcgag atgtgattcc
     1201 aggagatttt tgaatttcat ctccaaggca gactgatttt tatctttcat tcctgattat
     1261 ccgcactttt attgtcagtc tgatttcggc ctgttttcat tccagtgatt ttttcgctcc
     1321 agaccgaact cttttttcgt tccgaaatct gcagtattgc gaactcggca ttgttctcac
     1381 tgtccaagtg attccgtctg tcgatttacc tcggtgctgg tttgtgtaaa aacaaaaatc
     1441 gtatttcttc ttgacaaaga gacaatttca aacaaactat tgaatatgga caacttagct
     1501 cgcaagctct tcaccgaaga agaatacttg gaaaaggaaa gacaagcagc gaacaagagt
     1561 gaatactaca agggagaagt cttcgctatg gcaggagcga agcgaaagca taatgccatc
     1621 gtcactgcat tgatagccaa cctctatcca aaacttaagg acaatccctg tgaagtctat
     1681 ccttccgata tgaaagtaaa aaacaagaca gatcgatttt atacctatcc cgatgtaacc
     1741 atcgtctgcg gaaagccgga atttttggat gacaccgaag atgttcttac aaatcca
//
LOCUS       DAOQGJ010000872         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46790_length_1796_cov_2.550833, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000872 DAOQGJ010000000
VERSION     DAOQGJ010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46790_length_1796_cov_2.550833"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PK683_13125"
     CDS             complement(<1..1190)
                     /locus_tag="PK683_13125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH09436.1"
                     /translation="MKSGQNFPTVKILPKAEHSVKKFHPWIFSGGLNKVSKEIKAGSF
                     VRIADSHGNILGTGIYTPGDLSAVKIISFEKNFSLSVIHEILMISFEKRISLAEETDS
                     IRLLHGENDRIPGITIDWHSGTVVMRFYGKSLQKWARLISALSYSILKQFPEFPVENW
                     ILQKPVRTGKNSGADPENRNRVLRGKIPSDIEIHFRNAEFHLNPVMQKGGIFNDLRNL
                     KRYILEHRDSFQNHDVLNLFSNTGTLSVCLEKTGAKSITSAEISDECLDIHRVNLLDR
                     RRNRLVQMDIFKDLEVFLKKDSRMYDTIIIDPPSLTAKESDKIKARKVYKKLFYLTLP
                     FLNPDGIFIISSCSARIHENDFERIAREVFEQMNLNMKRIARLPQEIDHPVIPEFPEG
                     KYLKV"
     gene            complement(1380..>1796)
                     /locus_tag="PK683_13130"
     CDS             complement(1380..>1796)
                     /locus_tag="PK683_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09437.1"
                     /translation="YALAEEGALEKVNGFFQRIYESLENNGIFIFDILGNRHDYSGNF
                     IHERSEFSIFSRVTADKDIVKREIISFRKIKKSYRKSFEIHRLRMLDEDHLTGLLRDS
                     GFSVRKLEKYGKEPILPGRIAFECRKSLQQTRLGGN"
BASE COUNT          462 a          368 c          361 g          605 t
ORIGIN      
        1 actttcagat actttccttc cggaaattca ggaatgacgg ggtggtcgat ttcctgagga
       61 agccttgcaa ttcttttcat attcagattc atctgctcaa acacttctct tgcgattctt
      121 tcaaagtcat tttcatgaat tctggcagag catgatgata taatgaaaat tccgtcagga
      181 ttcagaaagg gaagagtcag ataaaacagt ttcttgtaga ctttcctcgc cttgatcttg
      241 tctgattcct ttgcagtaag gctcggagga tctataatta tggtatcata cattctgctg
      301 tcttttttca gaaagacttc cagatccttg aatatgtcca tctggaccag tctgtttctt
      361 cttctgtcca gaagattgac tctgtgaatg tccaggcact cgtcgctgat ttctgcagag
      421 gtgatggatt ttgctcctgt cttttcgaga catacagaaa gagttccagt attggaaaaa
      481 agattcagca catcgtgatt ctgaaaagaa tctctatgtt ccaggatata tcttttgaga
      541 tttctgagat cgttgaaaat ccctcctttc tgcatgaccg gattcagatg aaactcagca
      601 tttctgaaat ggatttcgat atcggaggga attttcccgc gcaggactct gttcctgttt
      661 tcaggatctg ctcctgaatt tttacctgtt ctgaccggtt tctgaagtat ccagttttcc
      721 acgggaaact ccggaaactg tttcagtatg gaataggaga gggcggaaat gagtctcgcc
      781 catttctgaa gagattttcc gtagaagcgc atcacgacag tgccgctgtg ccagtcgatt
      841 gtaattccgg gaatccggtc attttctccg tgcagaagtc ggatgctgtc tgtctcttct
      901 gcgagggaaa ttcttttttc aaagcttatc attaaaattt catgaattac tgatagggaa
      961 aaattctttt caaaggagat gatctttact gcagaaagat ctcccggggt atatatccct
     1021 gttcccagaa tgtttccatg gctgtctgcg attctgacaa aggagcccgc cttgatttcc
     1081 ttcgaaacct tgttcagtcc tcctgaaaaa atccaagggt gaaatttttt tacggaatgt
     1141 tccgccttgg gaaggatttt tacagtggga aaattttgtc cggatttcat atatgccatt
     1201 tctttccaag ccccgaataa atccagaggt tttagttctt atgtacaggg cattctgttt
     1261 ttgctccgat gctgaacatt gtgtctcttc tcaggcagaa gtgaaggagg gattgttgta
     1321 atgcagatta taatactttt ttcattacag tgatgtttct ttctcccagc aggagtcgtt
     1381 caattacctc ccagccgagt ctgctgtaaa gactttctgc attcaaatgc aattcttccg
     1441 ggaagaatcg gctctttgcc gtatttctca agttttctca cagaaaaacc ggaatctctc
     1501 agaagtcctg tcagatgatc ttcatcaagc attctgagtc tgtgtatttc aaaagatttt
     1561 ctgtaacttt tttttatttt cctgaaagaa ataatttctc tttttacaat atccttatca
     1621 gcagtcactc tggagaaaat ggaaaattca gaacgttcat ggatgaagtt tcctgaatag
     1681 tcatgtctgt ttcccagaat gtcaaaaatg aaaatgccat tattttctaa tgattcatat
     1741 attctttgaa aaaagccgtt gactttttca agcgcccctt cctctgcaag tgcata
//
LOCUS       DAOQGJ010000873         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46812_length_1795_cov_3.586782, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000873 DAOQGJ010000000
VERSION     DAOQGJ010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46812_length_1795_cov_3.586782"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PK683_13135"
     CDS             <1..1476
                     /locus_tag="PK683_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09438.1"
                     /translation="TGENMSFLFQLKSKKREKSSCGKIFFSFLLMSAVSVSAGDDPSV
                     LEFRNIWEKISENSHTQKALAMESKAAKLNSSRASKHWHPRIYADARAYNTNDPALNF
                     FSVLGQRAAKDSDFSTKSMRTRVSNFVDTNNNPYTVPNYNTLNLLAPDTLNNPGAHTY
                     QRGTFGFDLSVYEGGSKSSIAKAYDRQAEGKQLENKFVSLSEYANYAAMYGTISSLTE
                     SMEKIQALEKTVKNILGRYQLGNRGNPLGYSGGLGLKSLKNRIEGMKAETSARIESLR
                     DSIEVSSGKLPEKWGTRKQAVQAFADEYLKVKMAEQSYMARAMKMYAEGASEQAEAEK
                     ARVLPKVGLYGESYLYRGERASATAYNAGFYVQMNLYSAGDLDAVEQARLNSKAMQER
                     ANELIRNEESKAKSLLQMENALRKNLELVTENAKLMDEQVIVAQQLFGSGALNALQLS
                     EVLSRKADVIQVKAMTESEYLKVRAAMVTLSESGIEGVKHE"
     gene            1469..>1795
                     /locus_tag="PK683_13140"
     CDS             1469..>1795
                     /locus_tag="PK683_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH09439.1"
                     /translation="MSKLEKGFAGNLAEKFVNSRLTPVIAIVSIFLGVFSVYLTPKEE
                     EPQISVPMIDIQIPASGFEAKEVERKVTEPIERAVWGLDAVEYVYSASSAHGAVITVR
                     FKVGEPT"
BASE COUNT          555 a          340 c          460 g          440 t
ORIGIN      
        1 acaggagaaa atatgagttt tctgtttcag ttgaaatcta aaaaaaggga aaagagttcc
       61 tgcgggaaga tttttttcag ctttttgcta atgagtgctg tttctgtttc tgccggggat
      121 gatccgtcag ttctcgaatt caggaatatc tgggaaaaaa tcagcgaaaa ttcccacacg
      181 cagaaagccc ttgcaatgga atcgaaggct gcaaagctga acagcagcag ggcatccaag
      241 cattggcatc cgagaattta tgcagatgcc agggcataca atacaaatga ccctgcactg
      301 aactttttct ccgtgctcgg gcagagagca gccaaggatt ctgatttctc tacaaaaagc
      361 atgaggacca gggtcagcaa ttttgtggat acaaataata atccgtatac tgttccgaat
      421 tacaatactc tgaatcttct tgctcctgat actctgaata atcctggagc tcacacttac
      481 cagagaggaa ctttcggatt tgatctttct gtatatgagg gcggttccaa atcttcgatt
      541 gcaaaggcct atgaccggca ggctgaagga aagcagctgg agaacaagtt tgtatctcta
      601 agcgaatatg caaactatgc tgcaatgtac ggaacaataa gttctcttac agagtccatg
      661 gaaaagatac aggctctgga aaaaacagta aaaaatattc tcggcaggta ccagcttgga
      721 aacagaggaa accccctggg gtattccggc ggtctgggac tgaaaagtct gaagaacaga
      781 attgagggaa tgaaagcgga gacttccgca agaattgaat ccttaagaga ttcgattgaa
      841 gtctcttccg ggaaacttcc tgaaaaatgg ggaactcgaa agcaggcggt gcaggctttt
      901 gcggatgaat acctgaaagt aaaaatggca gagcagtctt atatggcaag agcaatgaaa
      961 atgtatgctg aaggtgcaag tgaacaggct gaggcggaga aagccagagt gcttcctaag
     1021 gtcggactgt acggagagag ttatctttac agaggtgaac gtgcatcagc aactgcttac
     1081 aatgctggct tttatgtgca gatgaatctt tattcagcag gagatctgga tgcagttgag
     1141 caggcaagac tgaacagcaa ggctatgcag gaaagagcaa atgaactgat caggaacgag
     1201 gaaagcaagg caaaatcgct tctgcagatg gagaatgcgc tccgtaaaaa tctggaactt
     1261 gttacagaga atgcgaagct tatggatgaa caggtcattg ttgctcagca gctttttgga
     1321 agcggtgctc tgaatgctct ccagctttct gaagtacttt ccagaaaagc agatgtgatt
     1381 caggtaaagg caatgacaga gtcggaatat ctcaaggtgc gggctgcaat ggtgacactt
     1441 tctgaatccg gtattgaagg agtgaaacat gagtaagctt gaaaaaggat ttgcagggaa
     1501 tcttgcagaa aaatttgtaa actccagact gacacctgtc atcgcaattg tcagtatctt
     1561 tctcggtgta ttttcagttt atctgacacc caaggaagaa gaaccgcaga tctctgttcc
     1621 tatgatcgac atacagattc ctgcttcggg ctttgaagcc aaggaagtgg aaagaaaggt
     1681 cactgaaccg atagaaagag ctgtatgggg actggatgca gtggaatatg tgtattctgc
     1741 aagttcagct cacggtgcag taattactgt ccgtttcaag gtgggtgaac caacc
//
LOCUS       DAOQGJ010000874         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46856_length_1794_cov_4.055779, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000874 DAOQGJ010000000
VERSION     DAOQGJ010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46856_length_1794_cov_4.055779"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK683_13145"
     CDS             <1..332
                     /locus_tag="PK683_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09440.1"
                     /translation="SHLILDFFSKKNYVLLIEEMQNDPTRLKIRAKLKREYFLETRMN
                     QKSNTLSFCLLKDEKRIWGLDYDVLRDWHIHPFENPDSHLNISPKTLPEILEIFHSIM
                     EELYSKE"
     gene            437..1351
                     /locus_tag="PK683_13150"
     CDS             437..1351
                     /locus_tag="PK683_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09441.1"
                     /translation="MTLHSCMHLICSGGSDLIETAIDESVKKRLALIHTIWILSPMAE
                     KKKSLLHEILSVEQDLKGNSEKARNQLIELFRSKQSHFTGMRRTFRPFAVDEEKGESS
                     GERVEAQTQLVTTVPEEIETAFGDIGKAIDLGFQIDEANTKAKADIIVNDEIIAKDVP
                     ATFLLQLEKRLREIRAVYKEIPTFDPVRVWSIDPGADKKHVLRAEPVTTIRKQKTRKY
                     KVMVEPTKEHPAQVDIMEVDEPVGEIKSYDWTGMLSQGQKTALIEQIDVLIEAVKRAR
                     SRANETEADVEKKISKQIFSFLLKPIKS"
BASE COUNT          600 a          354 c          399 g          441 t
ORIGIN      
        1 tctcacactt gattctggat tttttttcca aaaaaaatta tgttttactt atagaagaga
       61 tgcagaatga tccaacccga ttgaaaatca gagcaaagct gaagcgagaa tactttttag
      121 aaacaagaat gaatcaaaaa agcaatacac tttcattctg tctgttgaaa gatgaaaaaa
      181 gaatctgggg attggattat gatgttttga gagactggca tattcaccct tttgaaaatc
      241 ctgattctca cttgaatatc tctccgaaaa ccctgccaga gatactggaa atatttcaca
      301 gcattatgga agagctgtat tctaaagaat gataatgaga tattttcact ccagccgtcg
      361 agaggatacc agtaagccac gttgtctcct gcagctgttc cggacagcga atcaaaacgc
      421 acagacaagc aattagatga ctctgcacag ttgtatgcat ttgatttgtt caggcggttc
      481 ggatctgatt gaaacggcga tagatgaatc tgtcaaaaaa agacttgctc tgatccatac
      541 aatatggatc ctttccccca tggctgagaa aaaaaaatca ttacttcacg aaattttatc
      601 cgttgaacag gatctgaagg gcaattcaga aaaggcaaga aaccagctga tcgaactgtt
      661 cagatcaaaa cagagtcatt ttacaggaat gagacgtacc ttcagaccct tcgcagtgga
      721 tgaagaaaag ggtgaatcca gcggagagag agtggaagca cagactcagc ttgtcactac
      781 tgttccggaa gaaattgaaa ctgcgttcgg agatatagga aaggcgattg atctgggatt
      841 tcagattgat gaagcaaaca caaaggcgaa ggcggacatt attgtaaatg atgagattat
      901 agcaaaagat gtccctgcta cattccttct ccagctcgaa aaaagactga gagaaatccg
      961 tgcagtgtac aaggagatcc ctacttttga tcctgtgaga gtctggagca ttgatcctgg
     1021 agcagacaaa aaacatgtac tccgtgcaga accggtcact acaatcagaa agcagaagac
     1081 aagaaaatac aaggtgatgg tggaaccgac caaggaacat cctgcacagg tggacattat
     1141 ggaagtggat gaacctgttg gagagatcaa gtcctatgac tggactggaa tgctttctca
     1201 gggacagaag actgcactga ttgaacagat tgatgtactg atcgaggcag tcaaaagagc
     1261 acgctcgaga gcaaatgaaa ctgaggctga tgtggagaaa aaaatctcca agcagatttt
     1321 ttcctttctg ctgaaaccga tcaagtcctg atctgaaaaa aaatcaacca cggggagcac
     1381 ggggaaatct gtgctcttcg gcaatacaaa gtattgcatc tgtgacactc caccccggaa
     1441 ctccattttc gaaaaacgct tgacagtcgg ttgatgaaat atagtatgaa tatatagaaa
     1501 tactccacag gagggcaaaa tgtttttccg gcataacatc tttttcatca gaccaaagaa
     1561 atagggcacg gttcataatc tagccttcct gcatttactt ataggaccga gtgaaattat
     1621 gactccgtgc tgacctgctg aattcaaaat agcagattta aggagacatt gagtatgctg
     1681 caaaaacaaa aagccctaat cataggcgta aacctgaacg gccagccgga ctttcatgag
     1741 tccatggatg aacttcataa cctggcaatt gcctgcgaat ttaccgttgt cggc
//
LOCUS       DAOQGJ010000875         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_46979_length_1792_cov_2.479562, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000875 DAOQGJ010000000
VERSION     DAOQGJ010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46979_length_1792_cov_2.479562"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1487)
                     /locus_tag="PK683_13155"
     CDS             complement(48..1487)
                     /locus_tag="PK683_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNH09442.1"
                     /translation="MGKPTGFKEYQREYLSSEPAAVRVKNYKEFEKPFEEEKAKIQAA
                     RCMDCGIPFCHGETGCPVDNLIPEFNDLVYKGRWKEAIENLHSTNNFPEFTGRLCPAP
                     CEGACTLGLIAPAVSIKGIERTIIDRAFDSGLVQPIIAGKKTGKKIAVVGSGPAGLAA
                     AQQLARAGHEVTVYEKSDRIGGLLRYGIPDFKMEKWLIDRRVKQMEQEGVTFKTSVNV
                     GKDITGKELTSKYDAVVLAMGSEHPRDLNVPGRDLKGVHYAMEFLTLNNKSVAGDPVN
                     IISAKDKNVIVIGGGDTGSDCVGTSNRHGAKSVTQIEIFPEPPKTRDKDNPWPLYPRI
                     LRTSSSHEEGVQRKWSVNTLGFKGNDKGELTAIYGEQVEFKDGKMNSVPGSSFEWPAD
                     LVFLAMGFVHPVKEGLISQLCDLGMELDKAGNVKASFGRKEGSFATSLSGVYACGDVR
                     RGQSLIVWAISEGRKCAEQVHRYLLSEKK"
     gene            complement(1480..>1792)
                     /locus_tag="PK683_13160"
     CDS             complement(1480..>1792)
                     /locus_tag="PK683_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09443.1"
                     /translation="RNFAAGMSGGIAYVWDPKSVFERNLNKEMVELEKLTDPEEIDFV
                     LHHISKHKEYTDSKRAAELLSDWQNQISKFVKVIPTDYKKAMAKMKASNESKKGDLIH
                     G"
BASE COUNT          426 a          439 c          345 g          582 t
ORIGIN      
        1 aagattatca gtaattttat caatcgctga ccctgatcat tccgcagtta ttttttttct
       61 gataaaagat acctatgcac ctgttcggca catttccttc cttcagaaat tgcccagacg
      121 atcagggact gtcctcttct gacatccccg caggcatata caccggaaag tgaagttgcg
      181 aaagaacctt cctttcttcc gaaactggcc tttacatttc ctgccttatc cagttccatt
      241 cccaggtcac aaagctgaga aatcagtcct tctttgactg gatgaacaaa tcccattgca
      301 aggaaaacca gatctgccgg ccattcaaag ctgcttccag gaactgaatt cattttgccg
      361 tccttaaatt cgacctgctc accgtaaatg gcagtcagtt cacctttgtc atttcccttg
      421 aatccgagag tattcactga ccattttctc tgcacgcctt cttcatggct tgaggatgtt
      481 ctcagaatac gcggataaag aggccatgga ttatccttgt ctcttgtctt gggcggttcg
      541 gggaaaattt ctatctgagt tacagatttt gctccgtgtc tgttggatgt tccgacacag
      601 tctgatccgg tgtcaccacc cccaatgacg attacatttt tatcttttgc tgaaataatg
      661 ttcactggat caccggcaac agacttgttg ttcagagtaa ggaattccat tgcataatga
      721 actcctttca gatctcttcc aggaacattc agatctctag gatgctctga tcccattgcg
      781 aggactactg catcgtattt tgaggtgagt tcttttccag taatgtcctt tcctacattt
      841 acggaagttt tgaaagtgac gccttcctgc tccatctgct tgactcttct gtcaatcagc
      901 catttttcca ttttaaaatc cggaattccg tatctcagaa gtccgccgat cctgtccgat
      961 ttttcataga cagtgacctc atggcctgct cttgccagct gctgtgctgc cgcaagacca
     1021 gctggtcctg aacctacaac tgcaatcttt ttacctgttt ttttccctgc aatgatcggc
     1081 tgaacaaggc ctgaatcaaa agcacggtcg atgatcgttc tttctattcc tttgatggaa
     1141 actgctggtg caatcagtcc taaagtacag gcaccttcac agggagcagg acagagacgt
     1201 ccggtgaatt cagggaaatt atttgtagaa tgcaggttct caattgcttc cttccagcgt
     1261 cctttatata ccagatcatt aaattcagga ataagattgt ccacaggaca tcctgtttct
     1321 ccgtgacaga aaggaatacc gcagtccata catcttgctg cctgaatctt tgctttctct
     1381 tcttcaaaag gtttctcaaa ttccttataa ttcttgaccc tgaccgcagc cggttccgat
     1441 gaaagatatt ctctctgata ctctttaaag cctgtcggtt tacccatgga ttagatctcc
     1501 tttcttggat tcattggaag ccttcatttt ggccattgct tttttgtagt ctgtaggtat
     1561 tacctttacg aatttggaaa tctgattctg ccagtcggaa agaagctcgg ccgctctttt
     1621 agaatctgta tattccttat gtttggagat atggtgaagg acaaaatcga tttcttcagg
     1681 atcagtcagt ttttcgagtt ccaccatttc tttgttcaga tttctttcga agactgattt
     1741 cggatcccag acgtatgcta ttcctccgga cataccggct gcaaagtttc tt
//
LOCUS       DAOQGJ010000876         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47001_length_1791_cov_3.839286, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000876 DAOQGJ010000000
VERSION     DAOQGJ010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47001_length_1791_cov_3.839286"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /gene="rpsL"
                     /locus_tag="PK683_13165"
     CDS             <1..351
                     /gene="rpsL"
                     /locus_tag="PK683_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNH09444.1"
                     /translation="RHGRDKQKRKTKSPALKACPQRRGVCTRVMTFTPKKPNSALRKV
                     ARVRLSHGIEVTAYIPGESHNLQEHNVVLIRGGRVKDLPGVRYHIVRGTLDTLGVDKR
                     RKARSKYGAKKPKA"
     gene            365..838
                     /gene="rpsG"
                     /locus_tag="PK683_13170"
     CDS             365..838
                     /gene="rpsG"
                     /locus_tag="PK683_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNH09445.1"
                     /translation="MSRRRGKVEPRYMAPDSRYSDPVVTKFINCLMLSGKKSTAERIF
                     YDALDAIQAKLNTDPYKVFTEALGNVKPEVEVKSRRVGGVTYQVPIEVRPERRQALGI
                     KWMIRYAKDRNEKSMSAKLAAEIMDAHKGTGASIKKKEDIRKMAEANKAFSHYRW"
     gene            1000..>1791
                     /locus_tag="PK683_13175"
     CDS             1000..>1791
                     /locus_tag="PK683_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09446.1"
                     /translation="MFQRKFFLVVFLSVFFLIIGSCSVTRSISRSLSNKPFPKLKFTI
                     SDEHKKYFSYLNTSDDLYEIYEEFEKLEDLGDKTCEMVYEFVKRDDAQITKFIQNVKL
                     DPTPSKFSWTTGLYQRILAGCWNFYMMNDQKTDRDFLLVKKLYPNLKKNCYSVGFSQP
                     YMMHNILNCENLASMDMDWRILYAHSQMLKIFSEEKAKDSDTLSKAMAEMKIGWKAQY
                     VSKPIKRESPVNYGTFCDKGRTALCSEHFLKFQETLVTLKSFDFQL"
BASE COUNT          595 a          320 c          396 g          480 t
ORIGIN      
        1 agacacggca gagacaagca gaaaagaaaa actaagtctc ctgctttaaa agcatgtccc
       61 caaagaaggg gcgtttgtac aagagtaatg acatttactc caaaaaaacc aaattcagca
      121 ttgcgtaaag tagcaagagt aagactttct cacggtattg aagtgactgc ttatattcca
      181 ggagaatctc acaacctgca ggaacacaac gtagtattga tcagaggcgg aagggtaaaa
      241 gatcttcccg gggtaagata tcatattgtt cgcggaacac tggatacact gggtgtagat
      301 aaaagacgca aagctcgttc aaagtacggc gctaaaaagc caaaggcata attaggagta
      361 ttcgatgtcc agaagaagag gaaaagtaga acctaggtac atggcacctg acagcagata
      421 cagtgatcct gttgtcacaa agtttatcaa ctgtctgatg ctgagcggta aaaagagcac
      481 tgcagaaaga attttttacg atgcactgga tgcaattcag gctaaactga atacagatcc
      541 atacaaagtt tttactgaag cactcggaaa tgtaaaaccg gaagtggaag taaaatccag
      601 aagagtgggc ggggttacat accaagtacc tatagaagtc agaccagaaa gacgtcaggc
      661 attgggaatc aagtggatga ttcgttatgc aaaagacaga aatgaaaagt caatgagtgc
      721 gaaacttgct gcggaaatta tggatgctca taagggaact ggagcttcta tcaagaagaa
      781 ggaagatatt cgcaaaatgg cagaagccaa caaggcattc tctcattaca gatggtaatc
      841 agctcttcat cttattgatg aagaggcact ttctctttgc ggatagaacg tggagagatc
      901 ttcaggtaag cccataaagg agtctattga ctcctttttt tttacctatg caggtaatat
      961 gaaaaatttg gaaatggcag agtcctattg caggtgaata tgtttcagcg taagtttttt
     1021 ttggttgttt ttctttccgt cttctttttg ataatcggca gctgcagtgt tacaagatct
     1081 atttccaggt cgctcagcaa taagcctttt cctaaactga aatttacaat atctgatgag
     1141 cataaaaaat atttttctta tttgaataca tcggatgatc tttatgagat ttacgaggaa
     1201 tttgaaaaac tcgaagatct tggtgacaaa acatgtgaaa tggtctatga atttgtaaaa
     1261 agggacgatg cccagattac taaatttatt caaaatgtaa aactggatcc gactccctct
     1321 aagttttcct ggacaaccgg tctttatcag agaattcttg ccggatgctg gaatttttat
     1381 atgatgaatg accagaagac ggacagagac tttctgctcg tgaaaaaact ctaccctaat
     1441 ctgaaaaaga actgctacag tgtcggtttt tctcaaccgt atatgatgca caatattctg
     1501 aactgtgaaa atctggcttc gatggatatg gactggagaa tcctgtatgc tcattctcag
     1561 atgctgaaga ttttttcaga agaaaaggcc aaggattcag atacactttc aaaagcaatg
     1621 gctgaaatga agatcggctg gaaagctcag tatgtgtcta aaccgatcaa aagagagtct
     1681 cccgtcaatt acggcacttt ctgcgacaaa ggaagaactg ccctctgcag tgagcatttc
     1741 cttaagtttc aggagacact tgtgacattg aaatcgtttg attttcaact g
//
LOCUS       DAOQGJ010000877         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47042_length_1790_cov_3.254755, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000877 DAOQGJ010000000
VERSION     DAOQGJ010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47042_length_1790_cov_3.254755"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="fliM"
                     /locus_tag="PK683_13180"
     CDS             complement(<1..777)
                     /gene="fliM"
                     /locus_tag="PK683_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNH09447.1"
                     /translation="MTEILSQDEIDALLNAISSGEVNEEEYSAVGEQKKVKIYDFKRP
                     DKFSKDQIRTLQMMHETFARLATTGLSAQLRALVGVHVASVDQLTYEEFIRSIPNPTT
                     LAVINMDPLKGSAILEIDPSISFTIIDRLFGGKGESAKISRELSDIEMSVMEGIIVRI
                     LGNLRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKVGDVEGMTNLCIP
                     YITIEPIINKLSAQYWYSSIRRGETDENKAVIQERLDKVTI"
     gene            complement(774..1754)
                     /locus_tag="PK683_13185"
     CDS             complement(774..1754)
                     /locus_tag="PK683_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09448.1"
                     /translation="MFRLISVILSAFGTLLAYPEFDARGNSSENFIGLQGKVHLKLSK
                     MSYLPGEEIWADVTIRNYGNEVIRFYPAMPEIKTFQFAVTDENDTSLSLKDRHLMEDK
                     VLKRRNATVNLVGDSVKEIIIHKGESFTRRINLSEYFEFNPGKSYYVTGYFYPNSVED
                     SNHFLRTENKGIFFLENRRKEHRHKKYETESVKDSDVTPEEIVHLFLSAEMKKNWTNY
                     FKYIHFDQFINSYPNFSKEYAEADASGKEEITEEFKRFLKEEKAGKLSYYKVTEKNFV
                     SASLVKVHVNAEREMGRSPAKYEYIYTLRKADEGYSRIWKITNVIVKVRR"
BASE COUNT          481 a          384 c          340 g          585 t
ORIGIN      
        1 gattgtcacc ttgtcgagac gttcctggat gactgccttg ttttcatctg tttctccacg
       61 gcggatagac gaataccagt actgtgctga cagtttattg atgataggtt caattgtgat
      121 gtagggaata caaaggtttg tcattccttc tacatctccc acctttgttt ccagagtgat
      181 gagaaccacc atatcgtttg ggggcacaac ctgagcgaac tgaggatttg tttcaatgtt
      241 cccaagtctg ggacgcagat caatgactgt ggaccaggat tccctgaggt ttcccagaat
      301 tcttacaatg attccttcca taacggacat ttcaatatcg gaaagctctc tgctgatttt
      361 tgcagattcc cctttccctc cgaaaagtct gtcaatgata gtaaacgaaa tggatggatc
      421 aatttctaaa atggcagatc ctttcagagg atccatattg atgactgcaa gagtagtagg
      481 gttgggaatc gaccgaatga attcttcata agtcagctga tccactgaag ccacatgcac
      541 accgactagg gcccggagct gtgcagaaag tccagtcgtt gcaagacgcg caaatgtttc
      601 atgcatcatc tgaagagttc tgatctgatc ttttgaaaat ttatcaggtc ttttgaaatc
      661 gtatattttg acctttttct gctctcctac cgcagaatat tcttcttcat tgacttcacc
      721 gctcgatatt gcatttaaca gagcatcgat ttcatcctgt gataaaattt ctgtcatctt
      781 cttaccttga ctatcacgtt ggttattttc cagattcggg agtaaccctc gtctgctttt
      841 ctcaaagtgt atatatattc atattttgcc ggacttctgc ccatctctct ttccgcattg
      901 acatggacct ttaccagaga agctgagacg aagttttttt cagtgacctt ataataagac
      961 agttttcccg ctttttcctc tttcagaaac cgtttaaact cttcagtgat ttcttctttt
     1021 ccggaggcat cggcctcagc atattccttg gaaaaattcg gatacgaatt gataaactga
     1081 tcaaaatgaa tgtatttaaa atagtttgtc cagttttttt tcatttccgc agacagaaat
     1141 aaatgtacaa tttcttcagg agtcacatct gaatctttga ccgattcggt ttcatatttt
     1201 ttatgtctgt gttcctttct tctgttttcc aggaaaaaaa ttcctttgtt ttcagttctg
     1261 aggaaatgat tggaatcttc tactgaattt ggataaaaat aaccggtcac atagtaggac
     1321 ttcccagggt tgaattcaaa atactcgctc agattgatcc ttctggtaaa tgattcacct
     1381 ttatgaatga tgatttcttt caccgaatcc cccacaagat tgacagtggc atttctccgt
     1441 ttcagcacct tgtcttccat cagatgtctg tctttcagag aaagtgaagt atcattttca
     1501 tctgttactg caaattgaaa tgtcttgatt tcaggcattg caggataaaa ccgtatgact
     1561 tcatttccat agtttcggat agtcacatca gcccagattt cttctccagg aagatagctc
     1621 atcttgctga gtttaaggtg aaccttgccc tgcagaccga taaagttctc tgaggaattt
     1681 cccctggcat caaattccgg ataggccaga agcgtgccaa aggcagacag aatgacagat
     1741 atcagacgga acatataatt aaatcatcgg aaacaaaaag taaaatccat
//
LOCUS       DAOQGJ010000878         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47047_length_1790_cov_3.114121, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000878 DAOQGJ010000000
VERSION     DAOQGJ010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47047_length_1790_cov_3.114121"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..200
                     /locus_tag="PK683_13190"
     CDS             78..200
                     /locus_tag="PK683_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09449.1"
                     /translation="MMFSAAIALESKGLGYIFITMAPLTWDAALFQKRVVRQTC"
     gene            743..>1790
                     /locus_tag="PK683_13195"
     CDS             743..>1790
                     /locus_tag="PK683_13195"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH09450.1"
                     /translation="MKRLSISDRYTYCGILFGFLFPLLSTLFETYMQLGQISLLEMMH
                     IQEDHPLLWVIDTAPIFLGFTARLAGRKQEAAEKMNRQLKLLNRELNLEIIKNRTIEN
                     NLRDMISTYKEDLNSAKIVQEFMLPEIPKIPECKISYKYLPLNPVGGDLLSVIPLEEG
                     GLSILIGDVVGHGISAALIASLVKVFSNKNCRNFGTLPKLYMEQLNKEAGNYLPNDYF
                     FTALYGFLKFEKDSAKFIYSRAGHPYPFLFSHKAQKAGIIEVSGSPLGLMKNLHYSET
                     ETEVFPKDRIFLITDGLLEVRNQSGKFIGSEGFIEIINEICRQDLSLNESIEKILQRV
                     DEEGLYAPPEDDRLI"
BASE COUNT          568 a          346 c          379 g          497 t
ORIGIN      
        1 ttctgaaaat tacaggatct agatagcata tcgtatttgc gtaacgtgag ttactcactt
       61 cagccgtgta aaaagcgatg atgttcagtg ctgcaattgc actggagtcc aagggactgg
      121 gttatatttt cattacaatg gcacccttga cttgggatgc ggcacttttt cagaagcgtg
      181 tcgtacggca gacttgttga aaaaatcctg ccagcagaag acaaccgagt caattatcag
      241 tcgttcaaaa aatgctcata cgaacgggac atgaaatttt ccaggcggga aatggaaaac
      301 cggctctcca tttggctggc aggcttaatt tggatctgat tcttatggat tggcagatgc
      361 ctgagacgga tggactggaa gctgcagaat gcggagattg ccggttttgt gcagaatccg
      421 catattgcgc aggaacattc cggaaaccca aataaacttt tttcattata aataaatgaa
      481 acctgcctaa tgtctgattt aggtatattt ttaaatcatt ataaaataaa caaaaaccat
      541 tatctgcttt ctgcattcaa gcataaaatt cggatttctg ttcaaatgac aaaaaacatt
      601 atggatttct gcttgctttt acttcagcaa ttttgaaaat agtgagactg aatcaaaaac
      661 tgatatcatt cgcttttgct gtccggtaaa aaaagaatga aaaaagccat ttacgctgat
      721 cagccgatga actgaattaa atatgaaacg gctttccatt tcagacagat acacttactg
      781 cggaatactc tttggatttc tgtttccatt gctgtccact ctttttgaaa cctatatgca
      841 gctgggacag atttcgcttc tggaaatgat gcatattcag gaagatcacc cccttctatg
      901 ggtgattgat actgcaccta tattccttgg attcacagca agacttgcag gcagaaaaca
      961 ggaggcggct gaaaagatga acagacagct aaaattgctg aaccgggagc tgaatctgga
     1021 aatcatcaaa aacagaacaa ttgaaaacaa tctgagagat atgatcagca catacaagga
     1081 agatctgaat tccgccaaga ttgtgcagga gtttatgctt cctgagatcc cgaaaattcc
     1141 tgaatgtaaa atttcttata aatacctgcc gttgaatccg gtcggaggag atctgctttc
     1201 ggtcattccg ctcgaagaag ggggactcag cattctgatc ggcgatgtgg tcggacatgg
     1261 aatatcagca gcactgattg catctctcgt aaaagttttt tccaacaaaa actgccgaaa
     1321 ttttggaaca ctccctaaac tctatatgga acagctgaat aaagaggcag ggaattatct
     1381 gccgaatgat tattttttta ctgcactgta cggattcctg aaatttgaaa aagactcagc
     1441 taaattcatt tattccagag ccggtcatcc atatccgttt ttattttctc ataaggcgca
     1501 aaaggccgga atcatcgaag tctccggttc ccctctcggt ctgatgaaaa atcttcacta
     1561 cagtgagaca gaaactgagg tattcccaaa ggacagaatc tttttaatca ctgacggact
     1621 tttggaagtc agaaatcaat caggaaaatt catcggaagt gaaggattca tagagattat
     1681 aaatgaaata tgccgacagg atctttcact caatgagagt atagaaaaaa tactgcagag
     1741 agtggatgaa gaaggtttgt atgctccgcc ggaagatgac agactgatac
//
LOCUS       DAOQGJ010000879         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47050_length_1790_cov_2.964265, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000879 DAOQGJ010000000
VERSION     DAOQGJ010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47050_length_1790_cov_2.964265"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK683_13200"
     CDS             complement(<1..532)
                     /locus_tag="PK683_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNH09451.1"
                     /translation="MKETLSKRVIPCLDVKDGRVVKGIQFVNLRDAGDPVECAVTYQE
                     SLADELCFLDITASSDRRNILIHLVEEVAEKIFIPFTVGGGIRSVEDIREVLIRGADK
                     VSINTAAFQNPELLKNASELFGSQCIVCAIDARFNQERNRYEVYLHGGRTETGRDALD
                     WARQAEEFGAGEILLTS"
     gene            complement(529..>1790)
                     /gene="gatA"
                     /locus_tag="PK683_13205"
     CDS             complement(529..>1790)
                     /gene="gatA"
                     /locus_tag="PK683_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNH09452.1"
                     /translation="IQILSLIPIGIKDNICIEGERTTCGSKILEKFVSPYSAAVIDKL
                     LAKGFILFPGLNMDEFAMGSSTENSAYHITGNPFDTSRIPGGSSGGSAAGVAASMFPV
                     ALGSDTGGSIRQPASLCGIYGMKPTYGRVSRYGLVAYASSLDQIGPFSNDPEGIIDVL
                     SVISGLDMNDSTTVDVPASEKDKSPYSSWKGLRIGIMADEGAEWDKDVQNNFHKLISE
                     VKSQGAEIVELDFSVHKYSIPIYYILATAECSSNLSRFDGIRYGHRSDSRKLEDLYID
                     SRSEGFGKEVKRRILLGTFSLSSGYYDAYYGQAQKARAMIRKSYLDFFKKADFIFQPT
                     SPTAAFKIGEKMKDPIQMYKADILTTSANLGGVPSFSVPLGKGESGLPIGLQITGPHF
                     SEPKMVSLSKLIQSMEFTKIPLPREIR"
BASE COUNT          491 a          428 c          358 g          513 t
ORIGIN      
        1 tggaggtcag caggatttca ccagcaccga attcttccgc ctgcctggcc cagtcaagag
       61 catctcttcc tgtttctgtc cttccgccgt gcagatatac ttcatatctg ttcctttctt
      121 gattgaacct tgcatcaatc gcacatacaa tgcattgaga tccaaagagt tcacttgcat
      181 ttttcagaag ttcagggttt tgaaaagctg cagtattgat ggagaccttg tcggcacctc
      241 tgattaaaac ctcacgaata tcttcgacag atctgattcc gcctcctact gtaaaaggaa
      301 tgaaaatttt ctctgcaact tcttccacaa gatgaatcag aatatttctt ctgtcgcttg
      361 atgcagtaat gtccagaaaa caaagttcat cagcaaggct ttcctggtag gtcacagcgc
      421 actctactgg atcaccggca tctcttaaat tgacaaactg aattcccttc actacccttc
      481 cgtctttgac atccaggcag ggaatcactc ttttagaaag agtttctttc atctgatttc
      541 tctcggaagg ggaatctttg tgaattccat agactgtatc agtttcgaaa gggaaaccat
      601 tttcggttcg gaaaagtggg gtcctgtaat ctgaagtcca attggaagac cagattcccc
      661 ttttcccaat ggaacactga aagaaggaac tccgcctaaa tttgcacttg tagtcagaat
      721 gtctgccttg tacatctgaa tcggatcctt cattttttcc ccaattttaa atgctgcagt
      781 gggcgaggtc ggctgaaaga taaaatcggc ctttttgaaa aaatcaagat acgattttct
      841 gatcattgcc ctagccttct gagcctgacc gtaatatgca tcataataac ccgaagaaag
      901 agaaaatgtt ccgagcagaa ttcttctctt cacttctttt ccgaacccct cgcttctgga
      961 atcaatgtag agatcttcca gtttcctgct gtctgatcta tgaccgtaac gaataccgtc
     1021 aaaacgggaa agattggagg aacattcggc agttgcaaga atatagtaaa ttggaatgga
     1081 atatttatgt actgaaaaat ccagttctac aatctcagct ccctgagatt tcacttccga
     1141 gatcagttta tgaaaattgt tctgtacatc cttgtcccac tctgcacctt catcagccat
     1201 aattccgatt cgcagcccct tccaggaact gtaaggactt ttatcttttt cggaagcagg
     1261 tacatcgaca gtcgtgctgt cattcatatc cagaccggaa atcacactca gtacatcaat
     1321 aattccttcc ggatcgtttg aaaaaggtcc gatctgatca aggctggatg cataggcaac
     1381 aagaccgtat ctggatactc ttccgtaagt cggcttcatc ccgtatatgc cgcatagaga
     1441 agcaggctga cggatagatc cccctgtatc agaaccgaga gcgacaggga acatggatgc
     1501 ggcgacacct gctgctgagc ctccgcttga gcctcccgga attctggaag tgtcaaaagg
     1561 attgcctgta atatgataag cagagttctc ggtggatgac cccattgcaa attcatccat
     1621 attcagaccc gggaaaagta taaatccctt agccagaagt ttatcgataa cggcagcact
     1681 gtaaggtgag acaaatttct ccaggatctt cgacccgcag gttgttcttt ccccttcaat
     1741 acagatattg tctttaattc cgatcggaat taaagacaat atctgtattg
//
LOCUS       DAOQGJ010000880         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47066_length_1790_cov_2.638040, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000880 DAOQGJ010000000
VERSION     DAOQGJ010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47066_length_1790_cov_2.638040"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..785
                     /locus_tag="PK683_13210"
     CDS             69..785
                     /locus_tag="PK683_13210"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNH09453.1"
                     /translation="MGLYSLSPFQEIIPPVYDQISYLSQERIVGRKSQWAQTQMDVLD
                     IQGKQIASYTFRKRCGDSSKVYEMDNDYVFHCVSDEKRGNETVYSIIIAKNSKIQGTF
                     EKESSFEGLIKVKNPDAYINLKGEYIWKNLGTKTFSGKSAESYSIKDSAIIIDSSINR
                     YFPSKAKQAGISNRTVILSVEIDENGNMKKPEIVSGRAGYGFDEAALDLIQKVKWVPG
                     TKDGKPETMKVSVPVYFKLN"
     gene            890..>1790
                     /locus_tag="PK683_13215"
     CDS             890..>1790
                     /locus_tag="PK683_13215"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNH09454.1"
                     /translation="MKNMLIIVLLINYCAPLSKEGVRSRIKTDFQDAVKDNSEIIKGG
                     SLLVHSGKWDIHWKYAAGFTEKDGVKKNTVEDQPFHTASIGKTFTAAVIFQLIQEKKI
                     SLSDKIEKFFPKDYLKGLFNYDRTDWSPEVTVQHLLNHTSGIADYFGEYKDTPTLMKS
                     FIDEPDRFWTPKELVEFTRSNQKALSRPGELFHYTDTGYILLGMIVEKVTSKTFEKNL
                     EERFFIPLQMKNTYMHLRSKPLSRSNLDLSNIIFSKKDVTYFKSVSLDWAGGGLITTA
                     EDLLIFHKALREGKILSEKTVSEL"
BASE COUNT          599 a          339 c          367 g          485 t
ORIGIN      
        1 cctttgaatt aagcgtacca gctgattcgc aaaactcatc ttttgttttt tatcataaaa
       61 atcagaaaat gggactttat tccctgagcc cctttcagga aatcattccg cctgtttacg
      121 atcagatcag ctacctttcg caggaaagaa tcgtaggcag aaaaagtcag tgggcgcaga
      181 ctcagatgga tgtattggac attcagggaa aacagattgc atcctatact tttcgcaaaa
      241 gatgcggtga ctcatccaag gtatatgaga tggataatga ttatgtgttt cactgcgttt
      301 ctgatgaaaa aagaggaaat gaaacagtat acagcataat tatagcaaag aattccaaaa
      361 ttcagggaac ctttgaaaaa gaaagctctt ttgaaggtct gatcaaagtg aaaaatcctg
      421 atgcctatat taacctcaag ggcgagtata tctggaagaa tttaggaacg aagacatttt
      481 caggaaagag tgcggaatcc tattcaatta aggactcagc aatcatcata gactcaagca
      541 ttaacagata ctttccttca aaagcaaaac aggcaggaat ttcaaacaga acagtgattc
      601 tttctgtgga gattgatgaa aacggaaata tgaagaagcc ggagattgta tccggaagag
      661 caggttacgg cttcgacgag gctgcactgg acctgataca gaaagtaaaa tgggttcccg
      721 gcacaaaaga cggaaaaccg gaaacaatga aagtatccgt tcctgtctat tttaagctca
      781 actgaaaaac tttttctttc cagactgata tttttctttg ctctgcggca aagaaaaaga
      841 tagtcaaggc ttcaagcaga acaagcattg atctgtaagg tgactcttta tgaaaaatat
      901 gcttattatc gttttactta taaattactg tgctcccctc agcaaggaag gggtcagatc
      961 ccgaataaag acagattttc aggatgccgt caaagacaat tctgaaatca tcaaaggcgg
     1021 aagtcttctt gtgcattccg ggaaatggga cattcactgg aaatatgcag caggctttac
     1081 ggaaaaagac ggtgtcaaaa agaatacagt ggaagatcag ccctttcata ctgcgagcat
     1141 cggaaagaca tttactgcag cagtcatttt tcagcttatt caggagaaaa aaatttccct
     1201 ctctgataaa attgaaaagt ttttcccaaa ggattattta aaaggacttt ttaattacga
     1261 ccggacagac tggtcccctg aagtcacagt tcagcatctt ctgaatcata cttccggaat
     1321 tgcagattat ttcggtgaat acaaagatac tcctaccttg atgaaatcct tcattgatga
     1381 accggaccga ttctggactc ccaaagaact ggtagaattt acgaggagca atcagaaagc
     1441 cctttccaga cctggagaac tgtttcatta caccgataca ggctatattc tgctcggtat
     1501 gattgtggaa aaagttactt caaagacttt tgagaaaaat ctggaagaac gtttttttat
     1561 tccccttcag atgaaaaaca cctatatgca tctcagatca aagccgttaa gcagatctaa
     1621 tttagattta tctaatataa ttttcagcaa aaaggatgta acctatttca agagtgtcag
     1681 tctggactgg gcaggcggtg gactgatcac aacagctgaa gaccttctga ttttccataa
     1741 ggcactgaga gaaggcaaaa ttctttccga gaagacagtt tctgaacttt
//
LOCUS       DAOQGJ010000881         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47103_length_1789_cov_2.700692, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000881 DAOQGJ010000000
VERSION     DAOQGJ010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47103_length_1789_cov_2.700692"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PK683_13220"
     CDS             <1..715
                     /locus_tag="PK683_13220"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNH09455.1"
                     /translation="KSDFGVFTKSLAYGMEGKADKNGDGIVSFGELEEFVQNEVNDWS
                     LANNKQQKPFVRYYKEKYGDIPITVKGVREKSLVEKNSYNEGQSKLPFVWRSALLPGW
                     GQWYAGSKVRGMSYSFAALGALGYFASSYSGMNSAQSAYNSAMIIPGNTNFFLLTYMN
                     NQSKKSALESAQGTAQSAYFLVLGVWLWNLFDAGVITHKEKPKDSLSIDFNIHRYPVQ
                     VYSSNLIETHGSFEIFYKF"
     gene            727..>1789
                     /locus_tag="PK683_13225"
     CDS             727..>1789
                     /locus_tag="PK683_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNH09456.1"
                     /translation="MSKIIFLIIFLFFTNCIPQAKLNNPMHSAGLMTQLLNMVVNPQD
                     TSPPSFGGVSSVTSAGSNSILLSWTAAQDNVTPQNSIVYDIYMAPQKGWENYNVVSFS
                     TTGGATSFTAAGLSENTTYYFVVRARDSAGNRDSNTAELSAVTWPIPQILSVSTPNSE
                     LTAGDQITVSGTNFDSDPASYTLKLGACTLNVVNSTSTSITAAVPSDRTCTSGKIIFT
                     SKASNLSSIYPTDYKYFHNLLIVANTDGSVSSYLLNAASFPISARIETPSVFTPVAVG
                     TPVSIDYHSKYGMVYVQIWGQSNLIGNTLSDDGSIKFLSTYPSGSGTGTFAISPNSQF
                     MILGTVGSLATMYYYNTLLL"
BASE COUNT          521 a          369 c          365 g          534 t
ORIGIN      
        1 taaatctgat tttggggtct ttacgaaatc attagcttac ggaatggaag gaaaagcaga
       61 taaaaacggt gatggaattg tttcattcgg agaactggaa gaatttgttc agaatgaagt
      121 caatgactgg tctcttgcca acaacaaaca gcagaaacca tttgtccgat attataaaga
      181 aaaatatgga gacattccaa tcactgtaaa gggcgtcaga gaaaaaagcc tcgtggaaaa
      241 aaacagttac aatgagggac aaagcaagct tccttttgtc tggagatcgg cattgcttcc
      301 aggctgggga caatggtatg ctggatctaa ggttcgcggt atgtcatact cttttgctgc
      361 cctgggagct ttgggatatt ttgcatcctc ttactccgga atgaattctg ctcaatcagc
      421 ttataacagt gctatgatta taccaggaaa tacaaatttt ttccttctga cttacatgaa
      481 taatcagtca aaaaaaagtg cactggaatc ggcacaaggg acagcccaaa gcgcatattt
      541 tctcgttctg ggagtctggc tctggaatct ttttgatgcc ggtgtcatta ctcacaaaga
      601 aaaacccaaa gacagcttaa gtatagattt taatattcat cgctatcctg tccaggttta
      661 ttcttcaaat cttatcgaga cccatgggtc atttgaaata ttttataaat tttaaaggag
      721 atacttatgt ctaaaataat ttttctcatc attttcctct ttttcacgaa ctgcattccg
      781 caagcgaaac tgaataatcc aatgcacagt gcgggattga tgactcagct tctgaatatg
      841 gttgtaaatc ctcaggatac atctcctccg agcttcggag gagtgagcag tgtcacttct
      901 gccggttcaa attccattct cctcagctgg acagctgcac aggacaatgt gactcctcaa
      961 aattcgatcg tatatgatat ttatatggct cctcaaaaag ggtgggagaa ttataacgtt
     1021 gtaagtttca gtacaacggg cggagcgacc agctttaccg ctgcaggatt atccgagaat
     1081 acaacgtatt attttgtagt gcgggcaaga gacagtgccg ggaatcggga ttcaaatacg
     1141 gcagagctgt ctgcagtaac ttggccgatt cctcagatac tttctgttag tactccaaac
     1201 tctgaactca ctgcaggaga tcaaattact gtcagtggaa caaactttga ctccgatccc
     1261 gcaagctata cacttaaact gggagcctgc acactgaatg ttgtaaattc cacttccact
     1321 tcgattactg ctgccgttcc ttcggacaga acttgcacct ccggaaaaat tatttttacg
     1381 tcaaaagctt cgaatttaag ctcaatatat cctactgatt ataagtattt ccacaatctg
     1441 ctgatagttg caaacacaga tggatcagtt tcatcttacc tgctgaatgc ggcttctttt
     1501 cctatatcgg cgagaattga aactccttca gtctttactc cagtagctgt tggaacgcct
     1561 gtttccattg attaccattc aaaatacgga atggtctatg ttcagatttg gggtcaaagt
     1621 aatttgattg gaaatacttt atcggatgac ggcagtataa agtttttatc tacttatcca
     1681 agcggttcgg ggactggaac ttttgcgata tctcccaaca gccaatttat gattcttgga
     1741 acagtaggct ccttagcgac aatgtactat tataatacct tgctgcttc
//
LOCUS       DAOQGJ010000882         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47191_length_1787_cov_2.643764, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000882 DAOQGJ010000000
VERSION     DAOQGJ010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47191_length_1787_cov_2.643764"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PK683_13230"
     CDS             <1..955
                     /locus_tag="PK683_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09457.1"
                     /translation="NEAKNIPMVSLYMENILNIMDSERLGKRKAGEDLPPAPGEAQSE
                     ENEEESEEQTEENSETAQEMSPELSLGFKLMAETPVEALRKKHDPRNELAEFPDADKC
                     LLTYLFFKEFDHEYSIVMTTKKIIIQSTTVNGNKVDNKAKLLGIYELSRNCMDQFKIY
                     VDQAKEYFKAKLNPGSNYIEHSKKLTQLEQKRGQQSRNARATIREYIEKATDAFELLL
                     TDMRGERQIVGNMDEILTFDSVDSKKKMNRKAVKQCIMEAYSYSLAFYTRLSEGPHGD
                     LFGGVIELTPEQMQNIYGRAVVDNTSQPTVKPDDETSPLAT"
     gene            956..1666
                     /locus_tag="PK683_13235"
     CDS             956..1666
                     /locus_tag="PK683_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNH09458.1"
                     /translation="MSVIKVIIVQKNLSEPISSDQRSKISKERADFLILPRYYPSFKG
                     KDPSHEKEHLDAVLEVSEYHKGIVLGGSYFRKLEGTVLESAPLVKDVNLVDYSNVKNS
                     VKSSAADIDNGEYIYILNGIRFGVFIGEDFNNEAFFSELAKEKIELLFHIHSVPLGKD
                     DFSNYKKDLDKYAQISGKFGLNIIRCCGFGKFDGKEYSGRSLYSSPQGIKWKLGENEN
                     SSELIKTLNVSLSDRIPG"
BASE COUNT          585 a          326 c          385 g          491 t
ORIGIN      
        1 gaatgaagca aagaatattc ctatggtatc actgtatatg gaaaatattc tcaacataat
       61 ggactctgaa cggcttggga aaagaaaggc cggtgaagat ctgcctcctg ctccaggaga
      121 agctcaatct gaggaaaatg aagaagaatc agaagaacag acagaagaaa attcagaaac
      181 tgctcaggaa atgtctcctg aactttccct tggattcaaa ctgatggctg aaaccccggt
      241 agaagcactc agaaaaaaac atgatccaag gaatgaactt gcagagtttc ctgatgcgga
      301 taaatgcctc ctgacttatc ttttcttcaa ggaatttgat catgaatatt ccattgtaat
      361 gaccacaaaa aaaattatta ttcagtctac tacagtcaac ggaaacaagg tggacaataa
      421 ggcaaagctt ctgggcatct atgaactttc cagaaactgt atggatcagt ttaaaatcta
      481 tgtggatcag gcaaaagaat atttcaaagc caaattgaat cccggttcca attatattga
      541 acactccaag aagctgactc agcttgaaca gaaaagagga cagcagtcca gaaatgcaag
      601 ggcgaccatc cgtgagtata tagaaaaggc aacagatgcc tttgaacttc tgctcactga
      661 tatgcgggga gaaagacaga ttgtcggaaa tatggatgag attctcacat tcgattccgt
      721 agacagcaag aagaagatga acagaaaggc agtaaagcag tgcataatgg aggcatacag
      781 ttattcactt gctttctata ccaggctttc tgagggaccg catggggatc ttttcggagg
      841 agtgatagaa ctcactcccg agcaaatgca gaatatctat ggaagggcag tggttgacaa
      901 tacttctcag ccgactgtaa aacctgatga tgaaacctct cctttggcta cctgaatgtc
      961 agtcataaaa gtcattatag ttcagaaaaa tctgtctgag ccgatcagca gtgatcagag
     1021 aagcaaaatc tccaaggaaa gggcagattt cctgattctt cccagatact atccttcatt
     1081 caaaggcaag gacccttctc atgaaaagga acatctggat gctgttctgg aagtgtccga
     1141 atatcataag gggattgttc ttggaggttc ttattttcgg aaattggaag gaactgttct
     1201 tgaatctgct ccgcttgtaa aagatgtaaa tcttgtagac tattccaatg taaaaaattc
     1261 tgtaaaatcg tcagcggctg atatcgacaa tggagagtac atttatattc tgaacgggat
     1321 acgcttcggt gttttcatag gtgaagactt caacaatgag gctttttttt ccgagctggc
     1381 aaaagaaaaa attgaacttc tttttcatat tcattcggtt cctttaggaa aggacgattt
     1441 ttcaaattac aaaaaggacc ttgataaata tgctcagata tcagggaagt ttgggttgaa
     1501 tattatccga tgctgcggct tcggaaaatt tgacggtaag gaatattcgg gaagaagtct
     1561 ttattcctca ccgcagggca ttaaatggaa actgggagaa aatgaaaatt catctgaact
     1621 cattaagacc ttgaatgtaa gtctttctga cagaattccc ggataaataa tcagtcttcc
     1681 tgtatttttt tgacagccat acagctttca tctttcagga aaattccttt ttgtatttct
     1741 gtgcagtctg atttcagaat tagactgtga cagtttctga atgatgt
//
LOCUS       DAOQGJ010000883         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47195_length_1787_cov_2.524827, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000883 DAOQGJ010000000
VERSION     DAOQGJ010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47195_length_1787_cov_2.524827"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1623)
                     /locus_tag="PK683_13240"
     CDS             complement(1..1623)
                     /locus_tag="PK683_13240"
                     /inference="COORDINATES: protein motif:HMM:NF012323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWD domain-containing protein"
                     /protein_id="HNH09459.1"
                     /translation="MKRLIIFSTLILIGFFLNACDGKRKKGILLIPYFLMGADSSNTK
                     SAAVSSAGGGESSEHSGSNSSEHSGSGSSDDGSRGSEGSSRTCSVNNPGHSWGDPHLR
                     TLDGTMYDFQGMGEFWLIRTGEGKAGIQVRQEPWVNNKKVTVNTAVALAVGTHTVSIY
                     KSGKVYIDKVLTAFTSGKKVDLGDGTVSLSGKTYTISLKEGSIYTVTLNTTYINIYLN
                     SVKITGTIAGLLGNCNGNPKDDLIPRDETSAITINFNNLYRVFGESWRLRTNESWFEY
                     DAGQSLGSFDGLPDKITPLDPAAVATAETACKAKGITNTVLFDACVLDVALTGDPSFA
                     DAIAEVTPPVDGGSQIYYDVLAAITDTGSSGGNGSGGNGSSGNGSSGNGNGGNGNSGN
                     GNGGNGSGGNGSGGDDHHGNSCKKDDDRDEHHGYHHGDDDRDHHDGNRDDDDREGRYG
                     SSERRSSDHDGKYGDDDHKGKDREDRDRDNDRNRQRDRKCGTEKKRHHGYSGREGSDR
                     DVNERSRNHSSVKNHSGCSSIYTIWGHTRETL"
BASE COUNT          412 a          442 c          345 g          588 t
ORIGIN      
        1 ttagagagtc tcgcgagtat gaccccaaat tgtataaatg gaagaacatc cgctgtgatt
       61 ttttacagaa gagtgatttc tggaacgttc attcacatcc ctgtcagatc cttctcttcc
      121 gctgtaacca tggtggcgtt tcttctcagt accgcacttt ctgtcccgtt gtctgtttct
      181 gtcattgtca cggtctctgt cctcacgatc ttttccctta tggtcatcat cgccatattt
      241 tccatcgtga tcgctgcttc ttctttcaga tgaaccgtat cttccttcac ggtcgtcatc
      301 atcccggttc ccgtcatgat gatctctgtc atcatctccg tgatggtatc catgatgctc
      361 atctctgtcg tcgtcctttt tacaggagtt tccatgatga tcatccccac cgctgccgtt
      421 tccgccgctg ccgtttccac cgttaccgtt tccactgtta ccgtttccac cgttgccgtt
      481 tccactgctg ccgtttccac tgctgccgtt tccgccgctg ccgtttccac ctgatgagcc
      541 tgtatcagtg attgcagcaa gcacatcgta atagatctga gatcctccat caactggagg
      601 tgttacttct gcaattgcat ccgcaaaact tggatctcca gtcagtgcaa cgtccagtac
      661 gcaagcatca aaaaggacag tgttggtaat tcctttagct ttacatgcag tttctgcagt
      721 cgcgactgct gccggatcaa gaggagtgat tttatcagga agtccgtcaa atgatcctaa
      781 agactgacct gcatcgtatt caaaccagct ttcatttgtc ctgagtctcc agctttcacc
      841 aaatactctg taaagattgt tgaaattgat tgtgatcgca gaagtttcat ctcttggaat
      901 cagatcatct ttaggatttc cattgcagtt ccctaagaga ccggcaattg ttcctgtaat
      961 ttttacactg ttcaaataga tattgatata ggttgtattc aatgttacag tgtagatgct
     1021 tccttctttc agtgagattg tataggtttt gccggaaaga ctgactgtgc cgtctcccag
     1081 atccactttc tttcctgaag taaaggcagt aaggactttg tcgatgtaca cttttccgct
     1141 tttgtaaata gaaactgtat gagttcctac cgccaaagcg actgcagtgt ttactgttac
     1201 ttttttgttg tttacccaag gctcctgacg aacctgaatt cctgccttgc cttcacctgt
     1261 acggatcagc cagaattctc ccattccctg aaaatcgtac atagtaccat ccagtgttct
     1321 caggtgagga tctccccagc tgtgaccggg attgttcaca gagcaggttc tggaagatcc
     1381 ttctgatccc cttgaaccgt cgtcagatga acctgacccg gaatgttcgc tggagtttga
     1441 tccggagtgt tccgaacttt ctcccccgcc ggctgatgat actgctgctg attttgtatt
     1501 ggaagaatca gctcccatta aaaaataagg gataagaagt atcccctttt tcctttttcc
     1561 gtcacaggcg tttaaaaaaa atcctatgag gattaaagtt gaaaatataa tcaatctttt
     1621 cattcgcatt ctccacatac tgctgtactg ttcatatatt tagacgatac gatttctgca
     1681 gcaattacgg caaacactaa attttcttat ttaaaggaca ggtttttttg agtcttaacc
     1741 tgataaaatc agtcttagtc tttgagattg attatcaaat cctttat
//
LOCUS       DAOQGJ010000884         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47286_length_1785_cov_2.260116, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000884 DAOQGJ010000000
VERSION     DAOQGJ010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47286_length_1785_cov_2.260116"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PK683_13245"
     CDS             <1..768
                     /locus_tag="PK683_13245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH09460.1"
                     /translation="SQSFILSLRYSNAFKKAESLSIQLEKMGKVKDEFMSNLSHELRT
                     PLSLIYAYAEMMSERKGKNEEDIRNFGKDILREAGKLNENINDLMLLTDLESKFQLRN
                     ERIRASEICRDCTKYLKDAAEEKNIRLTVDVDEGLRFSADRSLISKVLIILVKNGIIY
                     NRPGGQVEVNVSQKNDKIVFSVLDNGIGISFADQSRVFEKFYRVDSSVTYAESGVGIG
                     LFIASRIAGLHNGEIILESAPDIGTSVQLILPKEPAV"
     gene            765..>1785
                     /locus_tag="PK683_13250"
     CDS             765..>1785
                     /locus_tag="PK683_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH09461.1"
                     /translation="MTFRRFLLDSLLRTIAFPRHFIFHSVEKTGEEHFLFEYPSIIAA
                     NHISPADVAAMPYLNRFTVSKEGYTIPAREDFLLPGFLVKEFRPRGVKKFFLKGIDSS
                     GIIPFLLKYVNTIPVKRPFRDNARELIKSGKMRDEVNQNWEILAEKIKEKNNLFLFPE
                     GTYSRDGYISQIRQGISIIRTKVPNLKINTVTLTYDFLSFKKPSLNVHIGKLEEFPDE
                     KTIKTSDFIRDRLGKGFAVTSGNIFSFILYSDELKTGMAESELFRKTSVFIKKLISEN
                     FIVSSTAKKLSESLFSQLTEKALAGGFLKKEEGLLKSCDSLYAEPEGKNKDFRKENTF
                     LYHRNQ"
BASE COUNT          593 a          357 c          384 g          451 t
ORIGIN      
        1 tcgcagtcat tcattctgtc actgagatat tcaaatgcat tcaagaaggc agaatccctc
       61 agcattcagc tggaaaaaat gggcaaggtg aaagacgagt ttatgtcgaa tctgtctcat
      121 gaactgagga cgcctctttc cctgatctat gcctatgcgg aaatgatgtc tgaacgcaaa
      181 ggaaaaaatg aagaggatat cagaaatttc ggaaaggata ttctgagaga ggctggaaaa
      241 ctgaatgaga atatcaatga tcttatgctc ctgacagatc tggaatcgaa gtttcagctc
      301 aggaatgaaa gaatacgggc atctgaaatc tgcagggact gcacaaagta tctgaaggat
      361 gctgcagaag aaaaaaatat caggctcaca gtggatgtgg atgagggact ccgtttctcg
      421 gcagacagaa gcctgatatc aaaggttctg atcattctcg taaagaacgg catcatttac
      481 aacagacctg gaggacaggt tgaagtgaat gtatcccaga aaaatgataa aatcgttttt
      541 tccgttctcg acaacggaat tggaatttca tttgcagatc agagcagagt gtttgagaaa
      601 ttctacagag tggattcatc cgtaacatac gctgaaagcg gcgtaggaat cgggcttttc
      661 attgcaagca ggatagccgg actgcacaat ggagaaatta ttctggaaag cgcaccggat
      721 atcggcacat ccgttcagct catccttccc aaggaacctg cagtatgaca tttcgcagat
      781 ttttactgga ctcccttctc aggacaattg catttccgag acatttcatt tttcacagtg
      841 tggaaaaaac aggtgaagag cattttcttt ttgaatatcc ctccataatt gcggccaacc
      901 atatcagccc tgcagatgtt gcagcaatgc cttatctgaa cagattcaca gtttccaagg
      961 aaggctacac aattccggcc agggaagatt ttcttctccc aggctttctc gtaaaggaat
     1021 tcagacccag gggagtcaaa aaattttttc taaaaggcat cgattcttca ggaatcattc
     1081 cctttcttct gaaatatgta aatacaatac cggtgaaaag acctttcaga gacaatgcac
     1141 gggaactgat taaaagcgga aaaatgagag atgaagtaaa tcagaactgg gaaatccttg
     1201 cagaaaagat aaaagagaag aacaaccttt ttctttttcc tgaagggacc tattccagag
     1261 acggttacat cagtcagatt cggcagggaa tctccatcat cagaaccaag gtcccgaatt
     1321 taaaaatcaa tacagtaaca ctcacttatg attttctgtc ctttaaaaaa ccgtctctga
     1381 acgtacatat aggaaaactg gaagaatttc cggatgaaaa aacaataaag acatccgact
     1441 tcattcggga cagactggga aagggatttg cggttacatc aggaaatatt ttttcattca
     1501 ttctttactc agatgaatta aaaacaggga tggcagaatc ggagcttttc agaaagacat
     1561 ccgttttcat caaaaagctg atttctgaaa atttcattgt cagctccact gcaaaaaaac
     1621 tttccgaaag cctgttcagt caactgacag aaaaggctct tgccggaggg tttctgaaaa
     1681 aagaggaagg acttctgaaa tcctgcgaca gcctctatgc cgaacccgag ggaaaaaaca
     1741 aagatttcag aaaggaaaat actttcctgt atcacagaaa tcagc
//
LOCUS       DAOQGJ010000885         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47315_length_1784_cov_3.781377, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000885 DAOQGJ010000000
VERSION     DAOQGJ010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47315_length_1784_cov_3.781377"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PK683_13255"
     CDS             <1..1114
                     /locus_tag="PK683_13255"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNH09462.1"
                     /translation="TAAKPSAVSSSTSTTTSTSTSTAAPVIKQPKFLVAANSALSNLC
                     VYSINSTDGSLSPVAGSPFATSATARFVIADSTGKFVYTANGTTAAISGYSINTSTGV
                     LSPIAGSPFAVPANPYSLVIDPSGKFLYAGNDQNTSIFLYTINTTTGQLTYSTNYTSG
                     TNAVAVTVEPSGKYLYAGWSGNPGKIDAFTINSSTGALTQVAGAPYTTGNDNISVAVD
                     PAGKFVFGANYFTTNVFSFVINTATGALSAAPGSPYAAGSAPGYVYSEPLGRFVYVAN
                     SGDAVGTAAISGYSLNSSTGALTAIGGSPFAAGMNPISFYSDPSGKYLYSVNTGSNNI
                     SGFAVNSSTGALTPVPGSPFSTGAGSSPFSIVMISY"
     gene            complement(1121..1708)
                     /locus_tag="PK683_13260"
     CDS             complement(1121..1708)
                     /locus_tag="PK683_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09463.1"
                     /translation="MKSIKYIIILLIIAGCGKEEPKQDHSGAVSSGYADSENCIDTKG
                     KNVFENFTEGCLGADCAAEACGEEKFIFRKDKSFYAEYSCHGPAFKGNWKADKKTLTV
                     EIVYSETKEEYCEDECTEEDGPNCISGCKKEFSRKYGREVLKTKLNYEFGDIGSNRIL
                     MKLETEDLNPVEGKEKYGESESPTEWEELGCMKKM"
BASE COUNT          411 a          458 c          352 g          563 t
ORIGIN      
        1 tactgcggca aaaccttctg cagtctcttc gagtacttcc acaactacgt ccacttcaac
       61 ctccactgcg gcccccgtca ttaaacagcc taagtttctt gtcgctgcaa attcggctct
      121 cagcaatctc tgcgtctaca gcatcaacag tacggatggc agcctgtctc ctgtggcagg
      181 ttctcctttt gcgaccagcg caactgcgag attcgttatt gcagattcta ccggaaaatt
      241 tgtatatacc gcgaatggaa caactgctgc catatcaggc tattcaatca atacaagtac
      301 gggtgtactg agtcctattg ctggatctcc ttttgctgtg cctgcgaatc cttattctct
      361 tgtcattgat ccatcgggaa aatttttgta cgcaggaaat gatcagaaca cttccatatt
      421 tctgtatacg ataaacacaa caactggaca gctgacctat tctacaaatt atacaagcgg
      481 gacaaatgcg gttgctgtga cagtggaacc ttccggaaaa tatctttacg caggctggag
      541 cggcaatccc ggaaagattg atgcctttac gatcaacagc tccaccggtg cactcactca
      601 ggtggcaggt gctccgtata caaccggtaa tgataatata tccgtagcag tggatccggc
      661 aggaaagttt gttttcggtg caaattattt tacgacgaac gttttttcat ttgtgatcaa
      721 cactgcaaca ggtgctcttt ctgccgcacc cggctcaccc tatgcagcag gctctgcacc
      781 gggatatgtt tattcagagc cgctcggcag atttgtatat gtggcaaaca gcggagatgc
      841 agtgggcact gcagcaattt ccggttactc tctcaacagt tctaccggtg cgcttactgc
      901 aataggaggc tctccatttg cagcaggaat gaatcctatc agcttttaca gcgatccttc
      961 aggaaagtat ctctattctg tcaatacggg ctcaaacaac atttcaggat ttgctgtgaa
     1021 ctcgtcaaca ggagcactga cgcctgttcc aggatctcca ttttccacag gtgccggttc
     1081 ttctcctttc agtattgtaa tgatttctta ttaaaaatca ttacattttc ttcatacatc
     1141 cgagttcttc ccattccgta ggggattcag attctccgta tttttccttt ccttccacag
     1201 gattcaggtc ctctgtttcg agtttcatca gtattctgtt tgatccgatg tctccgaatt
     1261 catagttgag ttttgtcttc agaacttctc ttccgtattt cctgctgaat tcttttttac
     1321 agccgctgat gcagttcggt ccgtcttctt cggtgcattc atcttcgcag tattcttcct
     1381 ttgtttcgga atatacaatt tccactgtca gtgtcttttt gtctgccttc cagttcccct
     1441 tgaatgcggg tccgtgacag gaatactctg catagaatga cttatccttt cggaaaatga
     1501 acttttcctc gccgcaggct tctgcagcac agtctgcccc gaggcagcct tccgtaaaat
     1561 tttcaaagac gttttttccc tttgtatcaa tgcagttctc actgtcggca tatcctgatg
     1621 aaaccgcccc tgagtgatcc tgcttcggtt cttcctttcc gcatcctgct atgataagca
     1681 gaattataat gtattttatt gatttcataa attctcctgg gattgataat aagtgcattc
     1741 cggatctgcc ttccagccct aaaaatcctt ctcttcatca tatc
//
LOCUS       DAOQGJ010000886         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47344_length_1784_cov_2.071139, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000886 DAOQGJ010000000
VERSION     DAOQGJ010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47344_length_1784_cov_2.071139"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1285
                     /locus_tag="PK683_13265"
                     /pseudo
     CDS             136..1285
                     /locus_tag="PK683_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            1287..>1784
                     /locus_tag="PK683_13270"
     CDS             1287..>1784
                     /locus_tag="PK683_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09464.1"
                     /translation="MKTIYTFFLFLIMQNLGAEKSPVISGCEIFPKNSVWNTPIDSLK
                     KDKNSDSYLKKIGLDKSLKADFGSGLWEGAPIGIPYNLSSGKEKKIHPEFEYLDESDK
                     GPYPVPVKPLIEGGPSSKGDRHILILDNKNCILYELYSAYPLREGGWKAGSGAVFDLK
                     STRLRP"
BASE COUNT          549 a          329 c          437 g          469 t
ORIGIN      
        1 caagtttgcg cgcgcaagcg agctgcagtt tttttttaga aaaattaaaa atagagttgt
       61 taaaactaat ttttcaacaa ctcaaatata cttactgagg cattctggaa ttgaatgcgg
      121 aagaggaatg aaataatgag actgaagaca ctggatgaaa ggcttgattt tttaaggaaa
      181 atcgaaatat ttaaggatct tgattataat atatttgtta aaattgcaga taatctgatt
      241 gaagtggaaa ttgctgagaa tgaatatctt ttccggaaag gggacagggg agattccatt
      301 ttcatagtat gcgacggcga actggaagca agggacggag agtttctcat tcgggttttc
      361 aggcagggat ttgtgctcgg agaatttgcc cttctcagca gtcagctcag gtcagcctca
      421 gtgattgcaa aaaccaaaag tcttctgctt cagctgactg acagattctt ttttgagatt
      481 ctgatgaatg acattacagt gcttcagaat attttaaaat cgcttgtgaa caggatactt
      541 ctggaagagc agaaaaatca gaaacttctt caggcaattc ttcctaagga tgtcgctgat
      601 gaactgaagg agaaaggtgc agctgaaccg gtgctccata aaaatgtctc tgttcttttc
      661 actgatttca aaggattcac tcagatttcg gaaacgatga cgccgcagga acttgtaaag
      721 gaactcgacg gatgtttttc acagtttgac agactcactg aaagatatca gctcgaaaag
      781 ctcaagacta tcggcgacag ttatatgtgc gtcggaggac ttcctgtgcc aaaacccgga
      841 aagatcatcg gtgcaaagaa tgccgtggac tcggtacttg cggcaatgga aattcaatcc
      901 tttatgcttc agatgaaaac cgtcagagaa gcacagggat ttccattctg ggagcttcgc
      961 ctgggaattc acagcggacc tcttgtggcg ggtgattggc cgcgataaga tcgcctatga
     1021 tgtctgggga gatacagtca atactgcgag caggatggaa tcaagcgggg ccgccggaaa
     1081 aataaatatc agcggtgcga cttatgaact ggtgaaggat ttcttcaact gcgggtacag
     1141 aggaaaagtg aatgccaaaa acaagggcga gctggatatg tattatgtat acggaatcag
     1201 gaaagaactg agttctgatt cttccgaaaa ttcgttcagt tcagttccga atgaaaaatt
     1261 ccgaaaactt tatgaggagc tttgacttga agacaatata tacatttttc ttatttttaa
     1321 ttatgcagaa tctcggtgca gaaaaatcgc cggtcatctc gggatgtgag atttttccga
     1381 agaacagtgt gtggaacaca ccgattgaca gtctgaaaaa ggataaaaat tcagactcgt
     1441 atctgaaaaa aataggactg gacaaatctc tcaaggcaga cttcgggagc ggtctctggg
     1501 aaggggcacc gatcggtatt ccgtacaatc tgtcttcagg gaaagaaaaa aagattcatc
     1561 ctgaatttga atatctggat gaaagcgaca aggggcctta cccggttcct gtcaaacccc
     1621 tgattgaagg aggaccttct tccaagggag accgtcatat tctcatactg gataataaaa
     1681 actgcatact gtatgaattg tacagtgcat atcctctccg cgaaggaggc tggaaggcag
     1741 gttccggcgc ggtatttgat ctgaagtcca ccagactgag acct
//
LOCUS       DAOQGJ010000887         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47368_length_1783_cov_3.664352, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000887 DAOQGJ010000000
VERSION     DAOQGJ010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47368_length_1783_cov_3.664352"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PK683_13275"
     CDS             <1..1263
                     /locus_tag="PK683_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09465.1"
                     /translation="LDDEIGHLLVSRDGWHSPRLIFNTWGRTVNTLAYMPIAAAPLWA
                     ARLWAAGMTVAASFFAWDFARRAGLRTAWTVPWFMAAQPAVLEGSWQALTLQPLMLAG
                     MALLWTGSAGYWKTAAMLAGCLPLIRHETIVLTGLFGLICLWRRKIYTGALAAVPILL
                     NFGAAWLFFGDNPLSIFFSPKPTEVYGSGDWTSLARPFINGTGSVLLIMAVIGGAAVR
                     NSALTALAVFTSVYFSIHAILFRFGLYATGGYAIFLLALAPGIALLGSFGFDWFIDVG
                     IVKIITLYAKLSDFRKPLKILIGALILSAVGAWAFTFHSIGWADEQNDAIDQGMAWLK
                     SSPYSKLPAEAAHVRFYYFMPRPIPVNPWLWMNSRPLESLPADTIVFRDAKYGDMFGF
                     PKEKLETPMFERLWASSDERVIIYRKKF"
     gene            complement(1450..>1783)
                     /locus_tag="PK683_13280"
     CDS             complement(1450..>1783)
                     /locus_tag="PK683_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNH09466.1"
                     /translation="RGTMGWVIFGLVWGIAVVGIIFKSFMTGKYDRLSTLLYVLAGWT
                     ILLDISHMYAVFPRTGFYWLFAGGVLYTGGAVFYLKDSMPRNHEIWHFFVLGASICHF
                     ICIFFYLI"
BASE COUNT          449 a          421 c          420 g          493 t
ORIGIN      
        1 ttggatgatg aaataggaca ccttttagtt tccagggacg gttggcattc tccccgactc
       61 attttcaata cttgggggcg aacagtaaat acacttgcct atatgcctat tgcagccgct
      121 cctctttggg cggcacggct ttgggccgca ggtatgacag tggcagcatc attctttgcc
      181 tgggactttg ccagacgggc aggactgcgg actgcctgga ctgtaccttg gtttatggcg
      241 gctcagcctg cagttctgga aggttcctgg caggcactca ctcttcagcc ccttatgctt
      301 gccggaatgg cactgctctg gacaggttca gccggttatt ggaaaactgc tgcaatgctt
      361 gcgggatgcc ttccgctgat tcgacatgag acaattgtgc tgacaggatt attcggactg
      421 atctgtctat ggcgcaggaa gatttataca ggtgcactgg ctgcagttcc gattctttta
      481 aactttggag cagcctggct ttttttcggc gataatcctc tgtctatttt cttctcacct
      541 aaacctacag aagtctacgg ttccggcgac tggacatctc ttgccagacc ttttataaac
      601 ggaacaggtt cagttcttct gattatggca gtcatcgggg gagctgctgt aagaaacagt
      661 gcgctcactg ctctggcagt ctttacgtca gtctatttct ccatacacgc aatactgttc
      721 agatttggac tgtacgcgac aggcggttat gcaatatttc ttctggccct tgcccccgga
      781 attgccttat tgggttcctt cggtttcgac tggttcattg atgtcggtat tgtaaaaata
      841 attactttat atgcaaagct ttcagacttc cgaaagcctt tgaaaatttt aattggagct
      901 ctgatactct ctgccgtcgg tgcatgggca ttcacctttc acagcatagg atgggctgat
      961 gaacagaacg atgcaataga tcaggggatg gcctggctga agtccagtcc ttattcaaag
     1021 ctgcctgcag aagcagctca tgtccgcttc tactacttta tgcctagacc catcccggtg
     1081 aatccctggc tctggatgaa cagcagaccg ctggaatccc tgcctgcaga tacaattgta
     1141 tttcgggatg caaaatacgg agatatgttc ggtttcccta aagagaaact ggaaacacct
     1201 atgtttgaaa gactttgggc ttcctccgat gaaagggtaa ttatctacag gaagaaattc
     1261 tgaacgggat tatcggagct gaagattcaa aaatgaatga ggataccgat agcggaggta
     1321 atgtcaggtt tctgttttat gcagagactt gacagaagcg aaaaagtcca atgctgtcaa
     1381 gttaaggcca ttgtcctgag tctgccgaac tatgaccttc atgacagcat ccttcgccga
     1441 actattatct cagatcagat aaaagaagat acatatgaaa tggcagatgc ttgctccaag
     1501 cacaaagaaa tgccagatct cgtggtttct aggcatactg tccttcagat agaaaactgc
     1561 tcctccggta tacagaactc cccctgcaaa aagccagtag aatcctgtgc ggggaaatac
     1621 cgcgtacata tgagatatgt ccagtagaat cgtccagcca gcgagaacat aaagcagagt
     1681 actgagtctg tcgtattttc ctgtcataaa acttttgaaa ataattccca cgacagctat
     1741 tccccagaca agtccgaata taacccagcc cattgtgcct ctc
//
LOCUS       DAOQGJ010000888         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47388_length_1783_cov_2.673611, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000888 DAOQGJ010000000
VERSION     DAOQGJ010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47388_length_1783_cov_2.673611"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PK683_13285"
     CDS             <1..1439
                     /locus_tag="PK683_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNH09467.1"
                     /translation="LSRLAEYKLECSEIREFCIRTLAFYKPIRSPYSSAYHISRRIFE
                     EGKTGKITTTLSREIQFNVSEIMKNNMNVLKKRNVKDAALLILENKTGYIKAYLGGTG
                     KNSENPDIDCVTSRRQAGSTLKPFIYALAFDRRLLTPASVLEDKPVEIQVGGGIYSPS
                     NYEEHFFGRVTARQALASSLNVPAVKTLDYIGTDVFADFLEKLGIRNLRESDFYGLSI
                     ALGSADVSLWDLTNAFRTLANQGIYSEASYYPGKRKEKKEILSSEAAFLVSDILADRE
                     SRALSFGLENPLSTRFPSSVKTGTSKDMRDNWCIGYSEYFTAGVWVGNINGAPMWNVS
                     GVTGAAHIWRDVMNYLHKNTEFHDKKIPAGIVRKSVRSGKNHFFEYFIPGTETDSIDP
                     APEKFNGIVSPVKNMIAALDSDIPSGRQKIPFMSKKPDSRNWWRLDERKISNAGKVYY
                     WKPAVGIHRLDLMNDKSEIIDSLRFEVR"
     gene            complement(1442..>1783)
                     /locus_tag="PK683_13290"
     CDS             complement(1442..>1783)
                     /locus_tag="PK683_13290"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNH09468.1"
                     /translation="VIMWATYVTTYGLTAKMLGLELNIFELLFTLFCGGMSLAVPSAP
                     SGIGVFHASVVSAFLLMGKDGNTGLVYATALHLVSFFTLTVSGLVFYLYWTYKRRHSG
                     KPVIEKEENHS"
BASE COUNT          554 a          359 c          417 g          453 t
ORIGIN      
        1 atctgtccag actggcagag tataaactgg aatgcagtga aatcagggaa ttctgcatcc
       61 gaactcttgc attttataaa ccgatccgtt ctccctattc atcagcatat catatttcca
      121 gaagaatttt tgaggaagga aaaacaggga aaattaccac cactctcagc agagaaattc
      181 agttcaatgt ttcagaaata atgaaaaata atatgaatgt acttaagaag aggaatgtaa
      241 aggatgcggc acttctgatt ctggaaaata agactggata tataaaggcc tatctgggag
      301 gaacaggaaa aaactctgag aaccctgata tagactgtgt gacttccaga aggcaggcag
      361 gctccactct taagcctttt atttatgcac ttgcatttga caggaggctg ctgactcctg
      421 cttccgttct ggaggacaag cctgtagaga ttcaggtagg cgggggaatc tacagtccat
      481 ccaattatga agaacacttt ttcggaagag tcactgcaag acaggcactc gcttcctcat
      541 tgaatgttcc tgcagtaaag actctggact acatcggaac agatgtattt gccgatttcc
      601 tggaaaagct tggaatccgg aatctcaggg aaagcgattt ctacggactt tccatagcct
      661 tgggatcggc tgacgtcagt ctctgggatc tgacaaatgc attcaggact cttgcaaatc
      721 agggaatcta ttccgaagct tcctattatc cggggaagcg gaaggaaaaa aaagagattc
      781 tgagcagtga agctgctttt ttagtatcag acattctggc ggacagagag agcagggcgc
      841 tcagcttcgg actcgaaaat cctctgagca cacggttccc aagctcagta aaaacaggca
      901 ccagcaagga tatgagagac aattggtgca tcggatattc tgaatacttt acggcaggtg
      961 tgtgggtggg gaacataaac ggagcaccga tgtggaatgt aagcggggtg accggcgctg
     1021 cacatatctg gagagatgta atgaattatc tccataaaaa tacagaattt catgataaaa
     1081 aaattccagc aggaattgtt agaaagtcag tgagaagcgg aaaaaaccat ttttttgaat
     1141 acttcattcc aggaacggaa actgattcga ttgatcctgc tccggagaag ttcaacggga
     1201 ttgtatctcc tgtaaaaaat atgattgctg ctctcgattc ggatattcct tcaggacggc
     1261 agaagatccc ctttatgtcg aaaaaacctg acagcagaaa ctggtggagg cttgatgaaa
     1321 gaaaaatttc aaatgcagga aaagtctatt actggaagcc tgcggtcggc attcacaggc
     1381 tggatctgat gaatgacaaa tcggagatta ttgattctct ccgatttgaa gtcaggtgat
     1441 ttcaggaatg gttttcttct ttttcgatga caggctttcc tgaatgtctt cttttgtaag
     1501 tccagtacag atagaagacc aggcctgata cagtaagtgt aaaaaagctt accagatgaa
     1561 gcgcagtagc atacactaat cctgtatttc cgtcttttcc catcaacaga aatgcggaca
     1621 ctactgaagc atgaaacacc cctattccgg aaggtgcaga aggaacagcg aggctcattc
     1681 ccccgcagaa cagagtaaac agcagttcaa atatattcaa ttcaaggccg agcattttgg
     1741 ctgtcagacc gtatgtagtg acatacgttg cccacataat tac
//
LOCUS       DAOQGJ010000889         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47394_length_1783_cov_2.416667, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000889 DAOQGJ010000000
VERSION     DAOQGJ010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47394_length_1783_cov_2.416667"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PK683_13295"
     CDS             <1..412
                     /locus_tag="PK683_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH09469.1"
                     /translation="TMNLSRKEIEERRDELLIELQIMRVANQQGFTLSGGERRRCEIA
                     RALATNPDFILLDEPFAGVDPIAVKDIQSVISSLKKRGLGILITDHNVRETLKITDRA
                     YIMYSGKILISGSTSDLVNDPETRRIYLGEDFTL"
     gene            415..>1783
                     /gene="rpoN"
                     /locus_tag="PK683_13300"
     CDS             415..>1783
                     /gene="rpoN"
                     /locus_tag="PK683_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016750890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNH09470.1"
                     /translation="MGLSQGLYQKQTQKLVLTQDLRNSIKLLELSNIELSEKIEFELE
                     ENPLLEEVYNPEKSKLNDLLSVYEKKKSEELDSFKSTDVAWQETAAYDMRRGDSDASN
                     RNQKFIESSAFAKNLGEYLMEQIRLTSLTENEVLLAEELISMLDGKGFIPESPEEIAE
                     LASVPADTVRKILNIMHRLDPLGIGARDIQQSLLVQAEILFPENYELQILLKDHFSDL
                     EKLDYKKISRSMKISEEGIMDLVKLVRKLEPFPATEYQEKKPNYIYPDIVVKETEGEF
                     SIFVNDEWIPKLSVNKEYKDLVKVTNSKDKEFITNRVNSAQSFIRSIHQRKQTMMKVM
                     NSIVELQIDFFKYGIHCLKPMTLKDIAERLGMHESTISRVTANKYVQTSWGVLELKWF
                     FSSSLKNSEGEDSHSSKKIQDLMRNLIKDEDKMDPLSDQEISEKISEMGADVARRTVS
                     KYRKIL"
BASE COUNT          584 a          333 c          410 g          456 t
ORIGIN      
        1 aacaatgaat ctttccagaa aggaaattga agagagaagg gatgaactcc tgattgaact
       61 tcagattatg cgtgtggcga atcagcaggg gtttactctt tcaggcggag aaagaagaag
      121 atgcgagatt gcacgggcac ttgccacgaa tcccgacttc atacttctgg atgaaccttt
      181 tgccggtgtg gaccctatcg cagtcaagga catacagtca gtcatcagtt ctctgaaaaa
      241 aagaggactg gggattctga tcaccgacca taatgtgaga gagactctga aaattacaga
      301 cagagcatat attatgtaca gcgggaaaat tctgatctcc ggttcgactt cggatctcgt
      361 aaatgatcct gaaaccagaa gaatttatct cggtgaggat tttactctgt aactatggga
      421 ctgagtcagg gattatatca gaagcagaca cagaagcttg ttctgactca ggatctgcgc
      481 aattcgatca agctgcttga actgtccaat attgaactct ctgaaaaaat agaatttgaa
      541 ctggaagaaa atcctcttct ggaagaggta tataatccgg aaaaatccaa actcaatgat
      601 cttctgtctg tttacgagaa aaagaaatca gaagaactgg attcattcaa aagtaccgac
      661 gtcgcatggc aggagactgc tgcttacgat atgagaagag gtgattcgga tgcctccaac
      721 cgcaatcaga aatttattga atcttccgca tttgcgaaaa atctcggcga gtatcttatg
      781 gagcagatca gactgacttc actgacggaa aatgaggttc ttcttgcaga ggaactgata
      841 tccatgctcg acggaaaagg ttttatcccg gaatcccctg aagaaattgc agaacttgct
      901 tcggtgcctg ccgatacagt cagaaaaatt ctgaacatta tgcacaggct ggatccgctg
      961 ggaattggag cgagagacat tcagcagtcc cttcttgttc aggcagaaat tctttttccc
     1021 gaaaattatg aacttcagat tcttctgaag gatcattttt ccgatttgga aaaactggat
     1081 tataaaaaaa tatccagatc aatgaaaata tcggaagagg gcattatgga tctggtgaaa
     1141 cttgtgcgga agcttgaacc atttcctgcc acagagtatc aggaaaaaaa accgaactat
     1201 atttatcctg atatagttgt gaaggaaacg gagggggaat tcagtatttt cgttaatgat
     1261 gaatggattc ctaaactctc agtgaacaag gaatacaagg atctggtaaa agtcaccaat
     1321 tccaaagaca aggaattcat tacaaacaga gtaaattcag ctcagtcatt catacgttcc
     1381 attcatcaga gaaagcagac tatgatgaaa gtgatgaatt ccatagtgga gcttcagatt
     1441 gattttttca aatatggaat tcactgtctg aagcctatga ctctgaagga tattgctgaa
     1501 cggctgggaa tgcatgagtc tacaatttca agagtgactg caaataaata tgtacagaca
     1561 tcatggggag tgctggagct gaaatggttt ttcagttctt ctctcaagaa ttcagaaggt
     1621 gaagattccc attcatccaa gaaaattcag gatctgatga gaaacctgat caaggatgag
     1681 gacaagatgg atcctctgtc cgaccaggag atctcagaga aaatcagcga gatgggagcg
     1741 gacgtagcac gcaggacagt ttccaagtac agaaaaatac tga
//
LOCUS       DAOQGJ010000890         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47486_length_1781_cov_2.364426, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000890 DAOQGJ010000000
VERSION     DAOQGJ010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47486_length_1781_cov_2.364426"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="nrfD"
                     /locus_tag="PK683_13305"
     CDS             complement(<1..696)
                     /gene="nrfD"
                     /locus_tag="PK683_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNH09471.1"
                     /translation="MTTAIKEKLGIVPLVEGGKDYKQVSDDILRPVDSFPTSLWWKAF
                     ILAVTVTVIDLGIIGYLMYEGLWILGINNPVGWGFFIVNFVFWIGIGHAGTLISAVLF
                     LFRQGWRTGINRAAEAMTIFAVLTAASTLIIHIGRPWLGYWLFPFPNERGPLWVNFRS
                     PLIWDTFAVSTYLSISLVFWYIGLIPDMAILRDRATHKVKKAIYSALSLGWVGSNRAW
                     SHYEIVCMLLAALS"
     gene            complement(704..>1781)
                     /locus_tag="PK683_13310"
     CDS             complement(704..>1781)
                     /locus_tag="PK683_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNH09472.1"
                     /translation="RTNVGKVGNGVGKNAYLLSELSNGRLVLSGMDASIEKTDKTYKL
                     ATTQDHHMMNPGSPLGTKWEDRPLIQHTAFENFQKNPESGKAPSEFPKITDKTKSDKP
                     FDAKGFNPEYQYKGYKWGMSIDLTLCTGCSACVIACQIENNIPVVGRDEVRVGREMHW
                     IRIDRYYMGDPDKPETVEIAHQPVMCQHCDNAPCETVCPVMATVHGSEGTNDMVYNRC
                     VGTRYCSNNCPYKVRRYNWMEHWVDGKDMARSPRNLAFNPEVTVRARGVMEKCTFCAA
                     RVAEKKIRAKNEGRKVRDGELKSACQETCPADAITFGDVNNKETAVSKNSSDPRSYRI
                     LEFLNVKPQVAYQTRIRNYVKENA"
BASE COUNT          469 a          423 c          383 g          506 t
ORIGIN      
        1 agatagagcc gcaaggagca tacatacgat ttcataatgt gaccaggccc ggtttgaacc
       61 gacccatcca agagaaagtg cactgtagat cgctttcttc actttgtgag ttgcacggtc
      121 cctgaggata gccatatcag gaatcagacc tatataccag aatacgagtg aaatggaaag
      181 ataggtagat actgcgaaag tatcccagat cagaggtgaa cggaaattca cccaaagagg
      241 gcctctttcg tttggaaacg ggaaaagcca gtaaccgagc caaggacgtc caatatggat
      301 aatcagagta cttgcagcag tcaagacagc gaaaattgtc atcgcttcag cagcacggtt
      361 gatccctgtt ctccatcctt ggcggaagag gaacagaacc gcagaaatca gtgttcctgc
      421 atgacctata ccaatccaga atacgaagtt tacgatgaaa aatccccatc ctaccgggtt
      481 gttgatcccg agtatccaga gaccttcata catcagatac ccgatgattc cgagatcaat
      541 gacagtgact gttacagcca gtataaaggc tttccaccaa agagaggtgg gaaaagagtc
      601 tacaggcctc agaatatcgt ctgaaacctg cttataatcc ttcccgcctt ccactaaagg
      661 aacaatgccc agtttttctt ttattgccgt ggtcattgac atattatgca ttctccttca
      721 catagttgcg aattcttgtc tgataagcaa cctgaggctt gacattcaag aactcgagga
      781 ttctgtaaga acgaggatcc gaactgtttt tagaaacagc agtttcttta ttatttacat
      841 caccgaatgt aatggcatct gccggacaag tttcctgaca tgcggacttg agctcaccgt
      901 cacgaacctt cctgccttca tttttagcac gaattttctt ctcagcaact ctggcagcgc
      961 agaaagtaca tttttccatc acccctctgg ctctcactgt cacttcaggg ttgaaagcga
     1021 ggtttctagg ggaacgtgcc atatcttttc cgtccaccca atgttccatc cagttgtatc
     1081 ttctgacttt gtaaggacag ttgttggaac agtatctggt tcccacacat ctgttataca
     1141 ccatatcatt tgtaccttct gaaccgtgaa cagttgccat tactgggcag actgtttcac
     1201 aaggtgcatt gtcgcagtgc tggcacatta caggctgatg ggcaatttcg actgtctcag
     1261 gcttgtcagg atctcccata taataacggt caattctgat ccaatgcatt tcgcggccta
     1321 cacggacctc gtcacgtcct acaacaggga tgttgttttc gatctgacat gcgatcacac
     1381 atgcggaaca tcctgtgcag agagtaagat caatggacat tccccatttg tagcctttgt
     1441 actggtattc aggattgaat ccctttgcat caaacggttt gtcggatttt gtcttgtcag
     1501 tgattttagg gaactcgcta ggggcttttc cgctttcagg attcttttgg aaattttcga
     1561 aagcagtatg ctgaatcaga ggtctgtctt cccattttgt tccgagagga cttccaggat
     1621 tcatcatatg atggtcctga gttgtagcga gtttataagt cttgtctgtc ttttcgatgc
     1681 ttgcatccat tccggacaga acaagtctgc cgttggaaag ttcagaaaga agataagcgt
     1741 ttttccctac cccatttcct acttttccga cattcgttct t
//
LOCUS       DAOQGJ010000891         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47528_length_1780_cov_2.882319, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000891 DAOQGJ010000000
VERSION     DAOQGJ010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47528_length_1780_cov_2.882319"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..>1780)
                     /locus_tag="PK683_13315"
     CDS             complement(209..>1780)
                     /locus_tag="PK683_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HNH09473.1"
                     /translation="KFNRSYTDALQGKIVIILPDNDETGKEFAEKVLRNIKKYCRALI
                     CNIPDLKEKGDVFDYLETHSFQDFKNLVTDKIPSLGTTDLDRSKRFVRKYKSEILSGE
                     NGWHIWTGKKWTPDDNRVMELAKYFSQNMADELTAKYKENPETNKQYKILSQTVSRER
                     SIKAIINLSRTDAEIQLKESALDSNINLLNLNNGTYDLVSHIFREHKQDDLLSNILPY
                     DYDPKAKCPIWEKFLNTVFEGNEELIKYIQKILGYTLTGETKLQAWFFLYGSGRNGKS
                     VFSEIISRLMDSYGTKIRTESIMAKEHNSENANPDIAKLKGKRLVIASEISDAHRLNE
                     SLIKDLTGGDKISARFLNRNPIEFSPQCKLFMYGNHKPFVRGTDDGIWRRIKLIPFKY
                     TIPENAVDPLLIEKLTKEISGILNWCLIGLKELQKQNYLINEPDIIKDETKAYRSEMD
                     VIGQFLEEYGYTYNQFYKEKMKTVYEDYTEYCKCNGYATLSQRRITDYLNSKGVMSAV
                     GSGNARIYQGIGKTA"
BASE COUNT          503 a          351 c          291 g          635 t
ORIGIN      
        1 aacgctccta tagcctgtat tttgcgaaat tcgggcatat tagggaatga tctaagactg
       61 aaagaagaaa agtcctgaaa aatgaaggtg ttaaccaagt taaccaagtt aactgatttc
      121 taggtctttt aaatggatac aaaatgaaat acaaaacaaa agaacctgaa ttttgattaa
      181 ctacattaac ttggttaact tttctcattt aggcagtttt tccgattccc tgatatatcc
      241 ttgcatttcc agaacccaca gcggacatta cgccctttga attgagataa tcagtaattc
      301 gacgttgtga taatgtggca tacccattgc atttacaata ctctgtatag tcctcatata
      361 cagttttcat tttctctttg taaaactgat tatatgtata gccatactct tccaaaaatt
      421 gccctatcac atccatttca gaacggtaag ctttggtttc atcttttata atatcaggct
      481 catttataag ataattctgt ttttgcagtt cctttaatcc aatgagacac caattcaaaa
      541 taccggaaat ttcctttgtg agtttctcga taagaagcgg gtcaacggca ttttctggaa
      601 tggtatattt gaacgggatt aattttatcc ttctccatat cccgtcatct gttcctctca
      661 caaatggttt gtgattgcca tacataaaca gcttacactg aggactgaat tctattggat
      721 ttcgattcag aaatcttgct gaaatcttgt cgccgcccgt tagatccttt atcagagatt
      781 cattcaatct atgagcatct gaaatttcag aagcaatgac aagccttttg cctttcagct
      841 ttgctatgtc tggattagca ttctctgaat tatgctcttt tgccatgatg ctttctgttc
      901 tgatttttgt cccgtatgaa tccataagcc tgcttatgat ttcactgaat actgattttc
      961 cattccgtcc agaaccgtat aagaaaaacc aagcctgcag ctttgtttca cctgtaaggg
     1021 tatagcctaa aattttctga atatatttaa tgagttcctc attcccttca aaaacagtat
     1081 tcagaaattt ttcccatatt ggacacttcg ctttgggatc ataatcatag ggcaggatat
     1141 ttgaaagcag gtcatcctgt ttatgctccc tgaatatgtg agatactaaa tcataagttc
     1201 cattgttaag gttaagaaga tttatatttg aatccaatgc tgattccttc aattgaattt
     1261 cagcatcggt tctgctcaaa ttgataatag cctttattga tctctctctt gaaacggtct
     1321 gagaaaggat cttatactgc ttgttagttt caggattctc tttgtatttc gcagtcagtt
     1381 catctgccat attctgagaa aaatattttg caagttccat tactctgtta tcgtctggag
     1441 tccacttttt acctgtccag atatgccagc cattttctcc agaaaggatc tctgatttat
     1501 atttgcggac aaaacgcttt gaccggtcta aatccgttgt tcctaatgat ggaattttat
     1561 cagtcacaag gtttttgaaa tcctgaaatg aatgcgtttc cagataatca aaaacgtccc
     1621 ctttttcttt caggtctgga atattgcaga ttaacgccct gcaatatttt ttaatgtttc
     1681 ttaaaacttt ttcagcaaat tcttttcctg tctcatcatt atcagggagt attattacaa
     1741 tcttgccttg cagagcatct gtataagacc ggttgaattt
//
LOCUS       DAOQGJ010000892         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47796_length_1774_cov_3.156486, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000892 DAOQGJ010000000
VERSION     DAOQGJ010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47796_length_1774_cov_3.156486"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PK683_13320"
     CDS             <1..1267
                     /locus_tag="PK683_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH09474.1"
                     /translation="VIGLRLPNERLLWINVNSEPILENDQFAGAVISFADITEKKIQE
                     EELNAAKKAADEGNRIKSEFLSIISHEIKTPLNSILGVTSLLLEEKPRPDQEENLSIL
                     KTSAKNLHTLISDILDYSELESGKARINEVEFDPAEYAQNILRSYSGKAEDKKINLNI
                     TIDSNTGKKTFGDALKFQQVLSNLLSNALKFTQNGSVSMDMKITEQDGMINVRTCVKD
                     TGIGIAEENLNRIFERFTQEVQTYSRKFEGTGLGLAITKKIIDLLGGSIQVESVLNEG
                     SSFTFQFPFKISAAEKKTVTEFSQPYHILLVEDDQTNAWLACKYMKKWNLITEHALNG
                     KEALKIADTIMFDLILMDIQMPEMDGFEAAEIMRSRNISCPILAFTAANISEIKERIF
                     ASGMNDFIVKPIQPSLMYKIMKKYLEKSD"
     gene            1264..>1774
                     /locus_tag="PK683_13325"
     CDS             1264..>1774
                     /locus_tag="PK683_13325"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNH09475.1"
                     /translation="MKNILILGSGGIASLYGSMMQRHGFSVSLYARRNSEEIASGIQV
                     DSVWGNYFFRPENVFTEIDFSKYRPDLIISSLKALPEIRLGTILKGLPEKIPVLLLQN
                     GIGLEEEFAELFPKNPVFAGLAFVCSNRHSPVYVNHLDYGQITVSPYLRSDMSLAEKI
                     YSVFKDSGVP"
BASE COUNT          594 a          351 c          385 g          444 t
ORIGIN      
        1 tgtaatcggt ctgagacttc caaatgaaag actgctctgg atcaacgtga actcagaacc
       61 catcctggaa aatgatcagt ttgcaggagc ggtcatttca tttgcagaca ttacagaaaa
      121 gaaaattcag gaagaggagc tgaatgcagc aaaaaaagca gcagatgaag gaaacagaat
      181 caagtccgaa tttctttcga tcatcagtca cgaaattaaa actcctctga attccatact
      241 tggggtcaca tctcttctgc ttgaagaaaa accgagaccg gatcaggaag aaaacctgag
      301 catcctcaaa acttcagcaa agaatctgca cactctgatt tccgacatac ttgactacag
      361 tgaactggag tcaggaaaag cgagaatcaa tgaggtcgag tttgatcccg cagaatatgc
      421 tcagaatatt ctgagaagtt attcaggaaa ggcggaagac aaaaaaatca acctgaatat
      481 aacaatcgat tccaacacag gcaaaaaaac attcggagat gcactgaaat ttcagcaggt
      541 tctttcaaat cttctgagca atgcactgaa atttacacag aacggatctg tttccatgga
      601 tatgaaaatc acagaacagg atgggatgat aaatgtaagg acctgtgtaa aggatacagg
      661 gataggcatt gcagaagaaa atctgaacag aatatttgag cggttcaccc aggaagtaca
      721 gacctattcc agaaagtttg aaggaacagg tctggggctt gcaatcacca aaaagatcat
      781 agatctttta ggcggatcca ttcaggtaga aagtgtactg aatgaaggct catccttcac
      841 ttttcagttt ccttttaaaa tatctgcggc agaaaaaaag actgtcaccg aattttcgca
      901 gccatatcat atccttcttg tagaagacga tcagacaaat gcatggcttg catgcaaata
      961 tatgaagaaa tggaatctga tcacagaaca tgcactgaac ggaaaagagg ctcttaaaat
     1021 tgcggacacc attatgttcg atctgatcct gatggacata cagatgccgg aaatggacgg
     1081 atttgaagct gccgaaatta tgagaagcag gaacataagc tgtcctattc tggcatttac
     1141 tgctgccaat atttctgaaa taaaagaaag aatatttgca tccggaatga acgatttcat
     1201 agtcaagccg atacagccca gccttatgta taaaataatg aaaaaatatc tggagaagtc
     1261 tgattgaaga acattctgat tcttggaagc gggggaatcg catctcttta tggaagcatg
     1321 atgcagaggc acgggttttc ggtcagccta tacgcaagaa ggaattcaga ggaaatcgcc
     1381 tcagggattc aggtagactc tgtatgggga aattatttct tcagaccgga aaatgtgttc
     1441 acagaaattg acttttccaa gtacagacct gatctgatta tcagctctct gaaagctctt
     1501 ccggaaatca ggcttggaac aatactcaaa ggcctccctg aaaaaattcc ggtgcttctg
     1561 cttcagaacg gcattggtct ggaagaggag tttgcagagc tcttccccaa aaatcctgta
     1621 tttgccggac ttgcctttgt atgctccaac cggcacagtc cggtgtatgt aaatcatctg
     1681 gattatggac aaatcacagt cagtccgtat ctaagatccg atatgtctct tgcagaaaaa
     1741 atttattccg tatttaaaga ttccggagtc cctt
//
LOCUS       DAOQGJ010000893         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47797_length_1774_cov_2.984293, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000893 DAOQGJ010000000
VERSION     DAOQGJ010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47797_length_1774_cov_2.984293"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1318
                     /locus_tag="PK683_13330"
     CDS             239..1318
                     /locus_tag="PK683_13330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNH09476.1"
                     /translation="MLNSLENPVYLIDSGFRVRYANQSALKFLKNSKEDLIGQKCYSA
                     IYKRNYICPYCPLQKYETSFEKLENLNQIPEKGLNTQILYSQKFGNQNLNLQFFPIQS
                     DEQLFIEQIQDITKYREKEEENLRMRNLASLGVMVSGVAHELNNPLTGISLTLQSLQN
                     RIEDIENLDIQERLELQSKYELMQSDLQKASQIVSEILSFARPEKRKMISSDIFDTIL
                     KAKETVERLNPSLCRKVRWEFIKEKDYVFYFDPMKMERVFINLFKNSLKAIDFKEGVI
                     SIEMKAKGSFCQISIEDSGGGIPDEIINKVFDPFFTSKKDNSGTGLGLSVCHSIIKEH
                     KGSISVMSYNKKTKFKIKLPMEMHP"
     gene            1315..>1774
                     /locus_tag="PK683_13335"
     CDS             1315..>1774
                     /locus_tag="PK683_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH09477.1"
                     /translation="MNERKKILVVDDNNSIRTAIADLLSKKYTCSSAENFDHAAEFLE
                     KEHFDLIITDIRMPGKSGLDLIEYAKSKITDTQYALITAYDINDYIHFAKNRGIWNII
                     PKYSSLDLNYITVMVDKLLYKDIFGPEKYFPGLQIIDSEKDSPFRPVQENC"
BASE COUNT          600 a          362 c          374 g          438 t
ORIGIN      
        1 caggtattga agcagacttt atgaaagctg gaaggagaaa aggaataaaa ttcgtcttct
       61 ttattttcaa taaaatcgct gtatccgagc gactcctctg aagatgtgat ttccagaccg
      121 cagagctggc acacaggata actcatggtc acgatattct cggaaaaccc ttgcttttgc
      181 aagcgaaaaa aaaaatttag actgacagga gacaaatctg gaccaaatat ttcagaacat
      241 cctaaattct cttgagaatc cggtctatct aattgattca ggattcagag tacggtatgc
      301 aaaccagtct gctttgaagt ttctgaaaaa ttcaaaggag gatctgatcg gacagaaatg
      361 ctactccgca atttataaaa gaaattatat ctgtccttac tgtcctctgc agaaatacga
      421 gacaagcttt gaaaagctgg aaaatctgaa tcagattcct gaaaagggtc tgaacactca
      481 gatcctgtat tcacagaaat tcggaaacca gaatctgaac cttcagtttt tcccgatcca
      541 gtcggatgaa cagcttttca ttgaacagat tcaggacatt acaaagtaca gagaaaagga
      601 agaggaaaat ctgaggatga gaaacctggc atctctcggc gtcatggttt caggagtggc
      661 tcatgaactt aacaatcctc tgacaggaat cagtcttaca ctgcagagtc tgcagaacag
      721 aattgaagac attgaaaacc tggacataca ggaaagactg gaacttcagt caaaatacga
      781 actgatgcag tccgatcttc agaaggcctc tcagattgta tctgaaattc tgagttttgc
      841 aagaccggaa aaaaggaaga tgatctcttc agacatcttc gatacaatac tcaaggcaaa
      901 ggaaactgtg gaaagactca atccttcact gtgcaggaaa gtgagatggg aattcattaa
      961 agaaaaagac tatgtatttt attttgatcc gatgaaaatg gaaagagtct tcatcaatct
     1021 tttcaaaaac tctctcaagg caatcgactt caaggaaggt gtcatctcca ttgaaatgaa
     1081 ggcaaaaggg agcttctgcc agatcagcat agaggacagc ggaggaggaa tcccggatga
     1141 aatcatcaac aaggtattcg atcctttttt caccagcaag aaggacaaca gcggaacagg
     1201 tctcgggctt tccgtctgtc attccatcat caaggagcac aagggttcga tcagcgtaat
     1261 gtcctacaat aaaaaaacca agttcaagat caaacttccg atggagatgc atccatgaat
     1321 gaaagaaaga aaatactggt tgtggacgac aataattcca tccggacggc aattgcggac
     1381 ctgctttcaa aaaaatatac ctgctcgtct gcagaaaact tcgatcatgc tgcagagttc
     1441 ctggaaaagg agcattttga tctgatcatc accgacatca ggatgcctgg aaaaagcggt
     1501 ctggatctga ttgaatatgc caagtcaaaa attacggata cgcaatatgc attaattaca
     1561 gcgtatgaca ttaacgacta tatccatttt gcaaagaaca ggggcatctg gaacatcatt
     1621 ccgaaatatt cctctctgga tctgaactat atcacagtca tggtggacaa acttctgtac
     1681 aaggacatat tcggaccgga aaaatatttt cccgggcttc agatcatcga ctcggaaaag
     1741 gattcacctt tccgtcccgt gcaggagaac tgcc
//
LOCUS       DAOQGJ010000894         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47807_length_1774_cov_2.611402, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000894 DAOQGJ010000000
VERSION     DAOQGJ010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47807_length_1774_cov_2.611402"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK683_13340"
     CDS             complement(<1..201)
                     /locus_tag="PK683_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09478.1"
                     /translation="MIFFRIAAFYFLVSVSVHSDSLKLNLGLWDGKYSTEFQERWKKD
                     NLIDGAPDGITKIDSSWSSRNMS"
     gene            complement(272..1669)
                     /locus_tag="PK683_13345"
     CDS             complement(272..1669)
                     /locus_tag="PK683_13345"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNH09479.1"
                     /translation="MKNISRKEFLGMAAASLVLGLDPLKKLEAAAPSLNDVVLINLLL
                     EGGPDFRHMIVPEPTTSYGQAYWKYRAGSQGVSSSSSTEWQNRFNQDYEKLSGTNGNP
                     SFGILKKCGWLIQKYKDGKAAIVCNVKHSESRDHQRSLLVLQKGNYSTAAFTTGGSGW
                     GGRVVGGANAETSAFSIVSVTPQIKPFCDGNSANVISFRNSRKFGLYTPSSLVNSWDN
                     NPSTDSKIVMYRALKTYYERRTGLDTTNFDAFKKHYDRLRAKTDQVSARISASKAPSG
                     ITGLSAGTGALNSADFAQQIQSAYDAFLCKDILNMRVLSMNYTGWDTHKRQKTEIEAK
                     LEDIFGTSKGLDQLFTNQSSAFSNSVISVSGEFGRQLRANGDNSTDHGDANYVLLIGG
                     RIAGGTYGELFPSAEIAKYDSYWQAIQSRNNFLAVYQSLLGIMGVTSTQVFNMTLTAG
                     TDYETGFGSFTGIAV"
BASE COUNT          449 a          424 c          366 g          535 t
ORIGIN      
        1 gctcatattt ctgctgctcc agctgctgtc aattttagtg atcccgtccg gtgcaccgtc
       61 tatcagattg tctttcttcc acctttcctg aaattctgta ctgtatttac cgtcccagag
      121 tcccagattc agcttcagac tgtctgaatg aactgatact gaaactaaaa agtaaaatgc
      181 tgcaattctg aaaaaaatca tattatttcc tattatcaaa aattatagta tatgtctgtg
      241 gaaaaacaga ctgaacgtgc ctctgaattc atcagactgc aattcctgtg aaactgccga
      301 accctgtttc atagtctgtc cctgcagtga gagtcatatt gaatacctgt gtcgaagtca
      361 ctcccatgat ccccagaaga ctctgataga cagcgaggaa attgtttctg gactggattg
      421 cctgccagta tgaatcgtat tttgctattt cagcggaagg aaaaagttca ccgtaagtgc
      481 ccccagcgat ccttcctcct atgagaagta cataatttgc atccccatga tcggtgctgt
      541 tgtccccatt ggctctgagc tgtcttccga attctccgga taccgatatg actgagtttg
      601 agaaagcaga agactggttt gtaaacagct gatccaagcc tttggatgtt ccgaaaatat
      661 cttccagctt ggcttcaatt tcggttttct gtcttttatg agtgtcccag ccggtataat
      721 tcatggaaag aactctcata ttcagtatgt ccttgcacag aaaagcatca taggcactct
      781 gaatctgctg cgcaaaatcg gcactgttca gtgctcctgt tcctgcggaa aggccggtga
      841 ttcctgaagg agcctttgag gcactgatcc ttgcactgac ctgatcagtc tttgctctca
      901 gtctgtcata atgctttttg aatgcatcga agttggtcgt atcgagacct gtacgacgtt
      961 cataataagt cttgagggca cgatacatca cgatttttga atcagtggaa ggattattat
     1021 cccaggagtt taccagactc gaaggagtat aaagtccgaa ttttctcgaa ttcctgaacg
     1081 agatcacatt tgcagaattt ccgtcacaga aaggtttgat ctgaggagtg acggatacta
     1141 tactgaatgc agaagtttct gcattggcgc ctcctacaac tcttcctccc catccgctcc
     1201 ctccagtcgt gaatgcggca gtggaatagt ttcctttctg aagcacaaga agcgacctct
     1261 gatgatctct tgattcggaa tgtttcacat tgcagacgat tgcagctttt ccatccttgt
     1321 acttctgaat cagccatccg cattttttca gaatcccgaa agaagggttt ccgtttgtgc
     1381 ccgataactt ttcataatcc tgattaaatc tgttctgcca ttcagttgag ctgctggaac
     1441 tgactccctg agagccagct ctgtacttcc aataggcctg tccgtacgat gtagtcggct
     1501 cgggaacgat catatgtctg aagtcgggac caccttcgag aagaaggttt ataagtacaa
     1561 catcattaag actcggtgca gcggcttcca gtttcttcag cgggtcaagc cccaggacaa
     1621 gcgatgcggc tgccattccc aggaattctt ttctgctaat attcttcatt tattttccct
     1681 cctgtacaaa cataaaagga agtgcaatga taaaatctat tgcaagtcct actcttgttc
     1741 tcgcttcatc acctttagtt ccggtatcat ccag
//
LOCUS       DAOQGJ010000895         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47855_length_1773_cov_2.696740, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000895 DAOQGJ010000000
VERSION     DAOQGJ010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47855_length_1773_cov_2.696740"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>1773
                     /locus_tag="PK683_13350"
     CDS             139..>1773
                     /locus_tag="PK683_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09480.1"
                     /translation="MTSIASDAEKLYSEYIKLSDAEQVIMELYSIQYFFIYSSQALQI
                     LQKLKIHKTLDKYSKEDFEDRIEKLKSKKLLAGRSKGENACNPEVSELITQNSIRKGT
                     FSRLAEEISATSHKRNISDENKLFALARINLYAGKEDIFEGLSDEFTSFRHYFQGLSA
                     EMEEELIELFLNPFSAEFLDSGVIHENYLEKIYISVIFNSVMNSIVSPEAAEYIVKKS
                     LKAELSLSAAGLVYEHLVLQGRNKDAENIFSKYFKKDFGEVLTVSAFKAFVEGNFSES
                     ERLYAAETKRIRTATKKKNEEMEGFSTFFYYASLVKLKEYKSAYEYSYKMMNLKYHFN
                     SDEFILLHSFSEMLNGSFREHSFRIPSPERFVSATHWQILIFFAAFYWMELNLSSGHL
                     QILQNRCRAAQSSGYRWFEMQFYDLTAKLTNDAVYSKKAEQIRNEIGSFYISEIYEKK
                     EKWMLALNALLTLNSAGGSAEKINYEKRIAWYIDYNDESGEFSILPYEQSFNPSKGWT
                     GGRPVALKRLKNEAAETPYLTEQDLRIISHIRLFSSG"
BASE COUNT          570 a          338 c          385 g          480 t
ORIGIN      
        1 aaaatacatt cttcagccat tctcagactg tatctttaca cagaaaactc attttataat
       61 ttacagcatt gttcagaatc tctttcctgt atatatgaga ttcggaagga acaccatttt
      121 gcagactgag gagcagcaat gacttcaatt gcatcagacg cggaaaaact gtattcagaa
      181 tatattaaac tgtctgatgc agaacaagtc atcatggaat tatattccat tcaatacttt
      241 tttatataca gcagtcaggc cctgcagatt ctgcagaagc tcaaaataca taagactctg
      301 gacaaatatt caaaagagga ttttgaagac aggattgaaa aactgaaatc aaaaaaactt
      361 ctggccggca ggtcaaaggg agagaatgcg tgcaatccgg aagtaagtga acttatcact
      421 cagaactcaa tccgcaaagg aactttttcc agacttgcag aggaaatatc tgcaaccagc
      481 cataagcgga atatcagtga tgaaaataag ctttttgccc tggccagaat caatctgtat
      541 gcaggaaagg aagatatctt cgaaggactg agtgacgaat ttacttcctt ccggcactat
      601 tttcagggtc tgtctgcgga aatggaagag gagcttattg agcttttcct taacccgttt
      661 tctgcagagt ttctggacag cggagtcatt cacgaaaact atctggagaa aatttatatc
      721 agtgtgatat tcaattctgt gatgaattcc attgtttcac ctgaggctgc agagtatatc
      781 gtaaagaaaa gtttaaaagc agaactcagt ctgtccgctg ccggactggt ctatgaacat
      841 cttgttctgc agggaaggaa taaggacgca gaaaacattt tctctaaata tttcaagaaa
      901 gacttcgggg aagtcctgac cgtttccgca ttcaaggctt ttgttgaggg aaatttttcg
      961 gaatctgaaa gactgtatgc agcggagaca aaaagaatca gaacagcgac gaagaaaaaa
     1021 aatgaagaaa tggaaggttt ttccactttc ttttattatg catcgctcgt aaagctgaaa
     1081 gaatataagt ctgcatatga atactcgtat aaaatgatga atctgaaata tcattttaat
     1141 tcagatgaat ttatccttct gcacagtttc agtgaaatgc tcaacgggag tttccgtgaa
     1201 cacagcttca ggataccttc acccgaaaga tttgtttcag cgacccattg gcagatcctg
     1261 atcttttttg ccgcatttta ctggatggag ctgaatcttt cctccgggca tctgcagatc
     1321 ctgcagaatc ggtgcagagc ggcacagagt tccggataca gatggtttga gatgcagttc
     1381 tatgatctga ccgctaaact gacaaatgat gcagtctact ccaaaaaggc agagcagatc
     1441 agaaatgaga tcggaagttt ttatatatca gagatttatg agaaaaaaga aaaatggatg
     1501 cttgcactga atgcgcttct cacgctgaat tctgccggag gaagtgcaga gaaaataaat
     1561 tatgaaaaga gaatcgcctg gtatatagat tataatgatg aaagcggtga attttccata
     1621 ctgccgtatg aacagtcgtt caatccttcc aagggctgga caggcggaag accagtcgca
     1681 ctgaagcgtc tgaagaatga ggcggccgag actccttatc tgacagagca ggaccttcga
     1741 atcatcagtc atatacggct tttttcctca gga
//
LOCUS       DAOQGJ010000896         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_47974_length_1770_cov_5.072886, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000896 DAOQGJ010000000
VERSION     DAOQGJ010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47974_length_1770_cov_5.072886"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="PK683_13355"
     CDS             <1..1515
                     /locus_tag="PK683_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH09481.1"
                     /translation="IPGAYLWSAGNTVLTFTPDSAYSAGSRYSFGISGSVKSASGTSI
                     GAVAPFSFLVNQELPPPFLTSVVLTTGTSSNCIPLSGTVNTDLLISSPMDACIGSVTR
                     NSIQFSFSEPMDTVSAQSGISFSPAVTGAYSWSAGNTVLTFTPDSAFNSDTRYSISFS
                     ALVKSSWKVSMTPISPLSFRTNPSLPAPTVTSITVTAGTVSSCLNLVGTSDLDILSNN
                     VQNACTGTVFRNPILISFSEPMDSASVIASLSLSPNFPHSLYWNAAKTVFTVLPDSAY
                     SFGSRVSLALGTVARSQNGISLSSTVTGSFVIGSVSSSPKVQSVGLKSQSCPNVAPGI
                     GNPSGGDWNLGSCFWDSSLPILGQSSYLFRAGDTGNGFSGSMNSCTDSDTDNFRIIFD
                     SYMDTAQVMNYVRLDRISPPFTTVRLASWEWTDCQASYPFGCRVLTLIYSEQEASCNG
                     ALFGNGTTGGDFNLMRTDNMPAGVPVYTLTVGSGARDANGISIGTDFRFSMEGK"
     gene            1512..>1770
                     /locus_tag="PK683_13360"
     CDS             1512..>1770
                     /locus_tag="PK683_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09482.1"
                     /translation="MMKYIQYISIFLFCIQCRSAGLKKAEIPSDAHEVFLGFIENRDT
                     KYGSSNARNIEDRLIFFLNRKGIQVRRNSSSAEKEKILQNGV"
BASE COUNT          434 a          437 c          406 g          493 t
ORIGIN      
        1 attccaggag cttatttgtg gtctgcggga aacacagtgc tgactttcac tcctgattct
       61 gcatacagtg cggggagcag atacagtttt ggaatttccg gctcagtcaa aagtgcttcc
      121 ggaacatcga taggtgcagt cgctccattc agctttcttg taaatcagga attacctcct
      181 ccttttttaa cgtccgttgt tctgacaact ggcacatcgt caaactgcat tcccctttcc
      241 ggaacagtga atacggatct tctcatttct tctccgatgg atgcctgcat cggctctgtt
      301 acaagaaact caatacagtt cagtttcagc gagccaatgg acactgtttc cgctcagtct
      361 ggaatttcct tctcacctgc agtgacaggt gcatactcct ggtcggctgg aaatactgtc
      421 ctgacgttta cacctgattc tgcattcaat tcggatacga gatattcgat cagtttttct
      481 gctttggtaa aaagctcctg gaaggtcagt atgactccaa tcagtcctct gagtttcaga
      541 acaaatcctt ctcttcctgc accgactgtg acttccatta cagtgactgc aggaacagtc
      601 tcttcctgcc tgaatcttgt cggaacatcc gatctggata tcctctcgaa caatgtgcag
      661 aatgcctgca caggaacagt gtttcgaaat ccgattctca tcagcttcag cgaaccgatg
      721 gacagtgcat ctgtaatcgc ttctctcagt ctcagtccga acttccctca ttcactctat
      781 tggaatgcag caaagactgt attcactgtc ctccccgaca gtgcgtattc tttcggatcc
      841 agagtcagtc tggctctcgg cacagttgcc aggtctcaga acggaatcag tctttcttcc
      901 actgtcactg gaagttttgt catcggatca gtttcctctt ctcccaaagt acagtcggtc
      961 ggattgaaaa gtcagtcctg tcctaacgtc gctcctggga ttggaaatcc ctccggcgga
     1021 gactggaatc tgggaagctg tttctgggac agcagtcttc ccatcctggg acagtcgagt
     1081 tatcttttca gggcgggtga tacagggaac ggtttttccg gttcaatgaa ttcctgtacg
     1141 gactcggaca cggacaattt cagaatcatc tttgattcct atatggacac ggcacaggtt
     1201 atgaactatg tgcgtctcga caggatatct cctcctttca caaccgtccg tcttgcaagc
     1261 tgggaatgga cggactgtca ggcatcttat cctttcggat gcagagtgct tacactgatc
     1321 tactctgaac aggaagcttc ctgcaacggt gcactgttcg gaaacggcac tacagggggg
     1381 gacttcaatc ttatgcgcac agacaatatg cctgcggggg tcccagtcta cacactcact
     1441 gtcggttcag gtgcgaggga tgcaaacggg atcagcatcg gcacagactt ccggttttct
     1501 atggagggaa aatgatgaag tatatacagt atatatctat ctttctgttc tgcattcagt
     1561 gcagatcggc aggactcaaa aaagcggaaa ttccttccga tgcacacgaa gtatttctgg
     1621 gattcattga aaacagggac acaaaatacg ggtcttcaaa tgccagaaac atagaagaca
     1681 ggctgatttt ttttctgaac aggaagggaa ttcaggtgag gcgaaattcc agttccgctg
     1741 aaaaggaaaa aattcttcag aacggagtat
//
LOCUS       DAOQGJ010000897         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48065_length_1769_cov_1.880397, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000897 DAOQGJ010000000
VERSION     DAOQGJ010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48065_length_1769_cov_1.880397"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..>1769)
                     /locus_tag="PK683_13365"
     CDS             complement(59..>1769)
                     /locus_tag="PK683_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH09483.1"
                     /translation="PNRIWASGSLLYALVYLLLSLRGIIHPMYSMVIGGTLNMTALSM
                     MYIAIKKFEMEEYSRFIPASVTLGMLVLFLYFQERPQSAAVSASFAISILTAVSSFAL
                     LSGKKSARFSFRTNGIVFLVCSFLFFVRGIYASLHSDFSLLSPHWINFSAFLSHFLVT
                     NFISLGFILMASEELYIELMEKNEQAQKANQAKSRFLANMSHEIRTPMNAILGISNLL
                     GETELTAEQKEYLQIISSSGSHLLSLINDILDLSKIESEKMDIENRKFNLKQNIETLK
                     NIFSVLASKKNLDFIFESEISLPESVSGDSLRLSQILTNLLGNAVKFTEKGSIALKIK
                     AGSSADEFIFTVSDTGIGIEEQKLSTVFDPFVQADSSATRNFSGTGLGLSISKELALR
                     MNGSISVKSVFGKGSSFTLKITLPRCTHSEDSAEVPSAPESVSLAKPVLRDVILADDE
                     STNRMIVLSYLKKYELNIHTAANGEETIQKFLTEPCQLILIDVQMPIKDGLSAAKEIR
                     KLEYLQKSEPVRIFTITANAFEEDIQNSLNAGCDRHLSKPIEKELLLNSIGEFFVLKQ
                     KVK"
BASE COUNT          491 a          352 c          381 g          545 t
ORIGIN      
        1 tagaagaacc tttccatatg caatctgaaa agggaattat acaattgcgt gtatccgttc
       61 attttacttt ctgtttcagt acaaaaaatt ccccgatgga attcagaaga agttcctttt
      121 caataggttt gctcaaatgc ctgtcgcacc ctgcattcag tgaattctgg atatcttctt
      181 caaatgcgtt tgctgtgatt gtaaatattc ttactggttc cgatttctgc agatattcca
      241 gtttcctgat ttccttggct gcgctcaggc cgtcttttat cggcatctgc acatctatca
      301 gtatcagctg gcagggttct gtcagaaatt tctgaattgt ttcttcgccg tttgctgcag
      361 tatgaatgtt cagttcatat ttttttaaat aagacagaac aatcattctg tttgttgact
      421 catcatctgc gagtatcaca tcccgaagca ccggcttggc aaggctgact gattcaggtg
      481 cactgggaac ctctgccgaa tcctcggaat gagtgcaccg aggcagtgtg attttcagtg
      541 taaaagagga gcctttcccg aaaacacttt tgactgagat gctcccgttc attctgagag
      601 caagttcttt tgagatcgac agtccaagtc cggtcccgga aaaatttcgt gtagcagacg
      661 agtctgcctg aacgaatgga tcaaagactg tgctgagttt ctgctcctca attccgattc
      721 cggtatcaga aactgtgaat atgaattcgt cagcagaact gcctgctttt attttgagtg
      781 caatacttcc cttttctgta aatttgactg catttccgag cagattggtc agtatctggc
      841 tcagtctgag agaatctccg gacacagatt ctggaagact gatttctgat tcaaaaatga
      901 aatccaggtt ctttttagat gcaagtacag aaaagatatt ttttaatgtt tctatattct
      961 gtttaagatt gaattttctg ttttcgatat ccattttttc ggattcaatc tttgacagat
     1021 ccagaatatc gtttattaaa gaaagaagat ggcttccgga agaggaaatg atctgaagat
     1081 attctttctg ttccgctgtc agttctgttt ccccaagtaa attagaaatt cctaatatag
     1141 cgttcattgg tgttctgatt tcatgactca tatttgccag gaatctgctt ttggcctggt
     1201 ttgccttctg tgcctgttca ttcttttcca tcagctcaat atagagctcc tcgcttgcca
     1261 taagaatgaa tcccagagat atgaagtttg tcaccagaaa atgggaaagg aacgcactga
     1321 aatttatcca gtgaggagaa agaagactga agtcggaatg aagagatgca taaattcctc
     1381 gaacgaaaaa aagaaaggaa catacaagga aaacaatgcc gtttgttctg aaggaaaacc
     1441 ttgcggactt ttttccggaa agaagggcga aactgctgac tgcggtgagg atgctgattg
     1501 caaatgatgc ggacacagca gcggactgag gtctttcctg gaaatataaa aacagaacca
     1561 gcattccgag tgttacagat gctgggataa atctgctgta ttcttccatt tcaaattttt
     1621 ttattgcgat gtacatcatg gaaagagcag tcatattcaa tgtgccgccg atgaccatgg
     1681 aatacatcgg atggatgata cctctcagtg agagaagcaa ataaacaagc gcatagagaa
     1741 gtgatccgga ggcccagatc ctgttcggc
//
LOCUS       DAOQGJ010000898         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48087_length_1768_cov_3.287799, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000898 DAOQGJ010000000
VERSION     DAOQGJ010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48087_length_1768_cov_3.287799"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..213
                     /locus_tag="PK683_13370"
                     /pseudo
     CDS             1..213
                     /locus_tag="PK683_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
     gene            complement(536..>1768)
                     /locus_tag="PK683_13375"
     CDS             complement(536..>1768)
                     /locus_tag="PK683_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09484.1"
                     /translation="PGDGRRESRMVELKKEVNKDLVEKNKDLDKKEDLDRLLTEKTKE
                     ERKALEEKKKEADRTEAKTEEKAKTAEEKLAELKKDPKADKNEIRKLADEKDKLDKKI
                     EEVKEEKKELAKKDEKISRTEKDLTGREIPKKEETVKIPSKDIPAKEAAKKDLPAKTD
                     PGAKSEAAAKSEAAAKSEAAAKTADTKTETLTAKETSSETKTDVSVPVKGTDAVSEEK
                     PEKTENILNGKIVYLQTVKYEEDGHYTNDLWVTDPEKLLTVKSSFKNISGRNFRIVPE
                     GIAVIGFDGNEPSSRVHHLVLLDELNLSMIRKTKEEIYWKSDIRENDGKLYAFEILEG
                     KVRLSRFNSDLSLDRRSSVPVYMNSEITFLKGKIYLTSKLQNSESKNLTVLKKDTMEV
                     IGTHRLSVIINEKKGSKK"
BASE COUNT          371 a          411 c          351 g          635 t
ORIGIN      
        1 gtgatctcag ggcatgtgca cgaggatcag ggagttgtaa agaaagggag gactgtgttt
       61 ttcaatccgt ccaatttcgg cggagttgat tccgtgttcg gataccagga aggcgggtat
      121 ttcggtgaaa tctatatgga acataaaaag gtgtttcagg taaaacttct gaaactgaaa
      181 ggggaagaaa taagagaact gtttgcggtg gatgcatcct caagtgttct caggacaatg
      241 cattttgatc cggattcggg gatttccgaa gagaattttg tgagaaaata atgggaatac
      301 agagatttaa gaatcatccg atcatccatg agttcaacgg tctgaagaag tttttcagaa
      361 agaatctctc ctatctgctc atgttctttt ttgacctttt cactcgcacg gttgaatgtc
      421 ctgttttcga agttcacttc cccggaaccc ttgacctctt cttcctcaag tctggccttt
      481 ccggtctgtg aatacagacc tatgctgaat aaaagaatga atgctgctgt aatcctcatt
      541 tcttgctccc ttttttctcg tttatgatga ctgaaagtct gtgagttccg atcacttcca
      601 ttgtgtcctt tttgagaaca gtcagatttt tagactctga attctgaagt ttggaagtca
      661 ggtaaatttt tcctttcagg aaagtgattt ctgaattcat atatacaggc acggaacttc
      721 ttctgtccag actcagatcc gagttgaagc ggctcagacg aacctttcct tccagaattt
      781 caaaagcata aagctttccg tcattttccc ttatatcgga tttccagtaa atttcttcct
      841 tggtttttct gatcatggag agattcagtt catccagaag gacaagatga tgcactcttg
      901 aagaaggctc atttccgtca aacccgatca cagcaattcc ctcaggaact attctgaaat
      961 ttcttccgct gatattttta aagctgcttt tgactgtgag cagtttttca ggatcagtca
     1021 cccaaagatc gtttgtataa tgaccgtctt cctcgtattt tactgtctgc agatatacta
     1081 tctttccatt taagatattt tccgtcttct caggtttttc ttccgaaact gcatcagttc
     1141 ccttgaccgg cacgctgaca tctgtctttg tttctgaaga agtttccttt gctgtcagag
     1201 tctctgtctt ggtgtcagca gtctttgcag ctgcttccga ttttgcagct gcttccgatt
     1261 ttgcggcggc ttccgatttt gcgccaggat cagtttttgc aggtaaatct ttctttgccg
     1321 cttcttttgc aggaatgtcc ttggacggaa tcttgacagt ctcctctttt ttggggattt
     1381 ccctgcctgt cagatccttt tctgttctgc tgatcttctc gtcctttttg gccagctcct
     1441 ttttttcttc cttgacttcc tctatctttt tatccagttt atctttctcg tcagccagtt
     1501 tacggatttc atttttatca gccttggggt cttttttcag ctcagcaagt ttttcctctg
     1561 cagtcttggc cttctcttcc gttttagcct cagtcctgtc agcttccttc tttttttctt
     1621 ccagagcctt tctttcttct tttgtcttct ctgtcagaag tctgtccaga tcctctttct
     1681 tgtccagatc cttgttcttc tcaaccagat ccttgttcac ttccttcttc agctcgacca
     1741 tccgggactc gcggcgacca tcgcccgg
//
LOCUS       DAOQGJ010000899         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48106_length_1768_cov_2.221249, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000899 DAOQGJ010000000
VERSION     DAOQGJ010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48106_length_1768_cov_2.221249"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PK683_13380"
     CDS             complement(<1..521)
                     /locus_tag="PK683_13380"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNH09485.1"
                     /translation="MKNSNFKERFLLLFRYAGAVFFVLMALRFAYGYFSFGEANVTDS
                     HPEQSFGQQNEFETGKRNYASDKGKKLPKEGGTAQFLSGQKYEKVGALNSKTDEFEKE
                     EKKLRGSIQKLGAQIQLEQNSGLAGRRMLRLGIGVDPGKFDEMVSEVRKIGNITSIQI
                     TKTDKTNEYRDLNA"
     gene            822..>1768
                     /locus_tag="PK683_13385"
     CDS             822..>1768
                     /locus_tag="PK683_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH09486.1"
                     /translation="MNELENFIPPFALSASCLTLYFAENRIMVLPFLILYFAFNLLMT
                     SLRNRSVQSKFLRLELIRFFINPLILFLIISFSGRNSVGWLLSLPGVIWAPLVTGSNL
                     VLLPVSFSYIAILGAAGYFTHSDLSFVMQQMLTVPSVLLLTLLLFGMIGDIQKVRIQN
                     ILQSIPLTVFTCNREGIIDFINRAIPPLSEADIIGRHILDFTPPHDHERVQGYFQKVT
                     EEGIIVNYDVDISQNASNVWLNVYISPILENGRVKETVFILQDVTEKRLMEIQLRTAK
                     DEAEKSSLIKSQFLAAMSHEIRTPLNGVIGFSDLLSRTSL"
BASE COUNT          494 a          413 c          313 g          548 t
ORIGIN      
        1 gcattcagat ctctgtactc attcgttttg tctgtctttg taatctggat actggtaata
       61 tttcctattt tccttacttc agaaaccatt tcatcaaatt ttccaggatc aacacctatt
      121 ccgagtctca gcattctcct cccggcaagt ccggaattct gttccagctg aatctgcgcg
      181 cccagtttct gaatggaacc tctcagtttt ttttcctcct tctcgaattc gtcggttttc
      241 gaattcaggg caccgacctt ttcatatttc tgtccagaaa gaaactgagc agtgccgcct
      301 tcctttggta actttttccc tttatccgat gcataatttc tcttgcctgt ttcaaattca
      361 ttctgctgac caaaagactg ttcaggatga gagtccgtga cattcgcttc tccgaaagaa
      421 aaatacccgt aagcaaacct taaagccatc agaacaaaaa aaaccgcacc tgcatatctg
      481 aacagaagca gaaatctttc cttgaaattt gaattcttca taataatctc ctgcagccga
      541 tataatctga attcgatttt cgaaaacgat tttttaacag atttcagcag tttgcacacc
      601 ctgatgatgc gtgcaggccg ctgaaaagac tgactgttgt ttcactctga tccgaatgcc
      661 gacctgcata agattcagtc agacagtaaa aaaagaattt attcacaagt acataaaaga
      721 tctgaaaata taatttactt ttaaactgac ctacgattca attattcttg ctcatgctga
      781 actttttcag aaaatccaga cctatcctgg aacaggaaaa attgaatgaa ctggagaatt
      841 ttattcctcc gttcgcactt tctgcttcct gcctgacgct gtacttcgca gagaaccgca
      901 ttatggttct ccctttcctg attctgtatt ttgcattcaa tttactgatg acttcactca
      961 gaaacaggtc agttcagtcg aaattcctcc gcctggagct gatacgtttt tttataaatc
     1021 ctcttattct ttttctgatc atcagcttct caggcagaaa ttccgtcgga tggctcctca
     1081 gtcttccagg agtaatctgg gcacctcttg tcacaggttc aaatcttgtt cttctgccgg
     1141 tttccttctc ttacattgca attttaggag cagccggata tttcactcat tctgatttaa
     1201 gcttcgtaat gcagcagatg cttacagttc cttccgttct tctcctgacc ctgcttcttt
     1261 ttggaatgat aggcgacata cagaaagtaa gaatacagaa tattctgcag tcaatacctc
     1321 tgacagtttt tacctgcaac agagaaggga tcattgattt catcaacaga gcaattcccc
     1381 ctctttcaga agcggacatt ataggcaggc atatactcga cttcacccct cctcacgacc
     1441 atgaaagagt acaagggtat tttcaaaaag ttacagaaga agggatcatc gtgaactatg
     1501 atgtagatat atcccaaaat gcttcaaatg tatggctcaa tgtttatatc tctcctatcc
     1561 tcgagaacgg aagagtaaag gagactgtat tcattcttca ggacgtgaca gaaaaaagac
     1621 tgatggaaat tcagctcaga acagcaaagg atgaagcaga aaaaagcagt ctgatcaaat
     1681 cgcagttcct tgctgcaatg agtcatgaaa ttcggacgcc gctgaacggg gtaatcggtt
     1741 tttccgatct gctttcaaga acatcact
//
LOCUS       DAOQGJ010000900         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48142_length_1767_cov_3.648949, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000900 DAOQGJ010000000
VERSION     DAOQGJ010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48142_length_1767_cov_3.648949"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PK683_13390"
     CDS             complement(<1..929)
                     /locus_tag="PK683_13390"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HNH09487.1"
                     /translation="MIMETFEEILVERTASLNVNPIYKLRISADGRVFYFGKAFSASI
                     GNHTWTISPKKISQINELLKSSSVFQFKYDGVEKGWAQDCESYSCFILMNNGRSREFT
                     DYCVFPKTLMSFQLELESIIGLSAYLYSNELTYYMLTEKSPNVKKDFHFLRAKSAEDA
                     VSLVHDSVYSHLSKKEPKWNITAVGKMQNRFRNDPYIYVSCLKEFSSEKILYLNHSEK
                     ISTANGSLFLFSERNSSYNDVSSDFHLVFAESADKALKLIQKTFPDRKNNLKYRNFAE
                     EKFIPQTDSFENSIIFSRLKSDYIIEEKWKPEC"
     gene            1394..1738
                     /locus_tag="PK683_13395"
     CDS             1394..1738
                     /locus_tag="PK683_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09488.1"
                     /translation="MPERKLGYRWVLFRFDGYIEDILMEGSFEPPVKDLGNGLNEEWV
                     LLNLNLEKCFDFEYTPKEGDNLILRSNDRYKYLSFTFEKNVWISNHYDPFSTVLEKFK
                     EGKIKNRDLSLQ"
BASE COUNT          547 a          308 c          327 g          585 t
ORIGIN      
        1 ctgcattcgg gtttccattt ttcttcaatg atatagtctg atttcagtct ggaaaaaata
       61 atgctgtttt caaatgagtc agtctgcgga atgaattttt cttctgcaaa gtttctgtat
      121 ttcagattgt ttttcctgtc cggaaatgtt ttctgaatca gtttcagggc cttgtctgcc
      181 gattcagcaa atactaaatg aaaatcagat gaaacatcat tgtaagaact gtttctttct
      241 gaaaacagaa acagtgaacc gtttgccgta ctgatttttt ccgagtggtt taaatacaga
      301 attttttccg aggaaaattc tttcaaacag gatacataaa tgtacggatc atttctgaat
      361 ctgttctgca tttttccgac tgcagtaata ttccatttgg gttctttttt cgacagatga
      421 gaataaactg aatcatggac taggctgaca gcatcttctg cagattttgc acggagaaag
      481 tgaaagtctt ttttaacatt cggacttttc tctgtaagca tataataagt gagctcattt
      541 gagtaaagat aagcagaaag accgatgatg gattccagtt ccagctgaaa actcatcagt
      601 gttttgggaa atacacagta gtctgtgaat tctctggagc gtccattgtt catcagaata
      661 aagcagctgt agctttcgca gtcctgcgcc cagccttttt cgacaccgtc atatttaaac
      721 tggaaaacac tggagctttt cagcagttca ttgatctggc tgattttttt ggggctgatt
      781 gtccaagtgt gatttccgat tgaagctgaa aaggcttttc caaaatagaa cacccttcca
      841 tctgctgaaa ttcggagttt atagattggg tttacattta gagatgcagt tctttctacc
      901 agaatttctt cgaaagtttc cattatcatt tttaactcct ctcagcagat caactgaagt
      961 atgcttttca tcccctcatt ccattgacta aactcaagcg gatactcttt aactccaatc
     1021 aaaccggata ctttgaatcc ttttgagcac tgcttttaag tattttttat tttcgtaaag
     1081 cagtctcaga ttatagatca gttttggata tatagttcaa aaaatcctca ataatccata
     1141 ataacaccaa actaaatcat tgattttatt tttcaacctt ttatgtaatt tttaataaaa
     1201 aataataaaa atgacataaa taatcgaaat atcttaaaca gagaaatcgg ttgaaaaaag
     1261 ggttgctcaa tattgtccgc gccctattct ttttcactgt atgtgtgatt tcgaaaatgg
     1321 ctttatcctg tgttcctgtg agccgctggt cagcattgag ccgccaaaaa gaaaatcgaa
     1381 aaagaatcaa aatatgccgg agaggaaact tggatacaga tgggttctgt tccggtttga
     1441 cgggtacata gaagacatac tgatggaagg aagttttgaa ccgcccgtaa aagatctcgg
     1501 gaacgggctc aacgaggaat gggttctgtt gaatctgaat ctggaaaaat gtttcgattt
     1561 tgagtatact ccaaaagaag gggacaattt aattttaaga tcgaatgaca gatataaata
     1621 cctttcattt acatttgaga aaaatgtatg gatcagtaat cattatgacc cattttccac
     1681 agttttggag aaattcaaag aaggcaaaat taagaataga gatctttcgc tccaatgata
     1741 aagataataa tatttatatt taatgtc
//
LOCUS       DAOQGJ010000901         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48192_length_1766_cov_2.932788, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000901 DAOQGJ010000000
VERSION     DAOQGJ010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48192_length_1766_cov_2.932788"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PK683_13400"
     CDS             <1..251
                     /locus_tag="PK683_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09489.1"
                     /translation="STGPSKENTVGFAVSMAVFVLLFWTSFRVLPYHTLLTEYTSEIR
                     QSAMFGWVPELFYASVALVILSFLWMVYSGLVLIRNSR"
     gene            256..1131
                     /locus_tag="PK683_13405"
     CDS             256..1131
                     /locus_tag="PK683_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09490.1"
                     /translation="MLFKLLLRLLTPPRIFFRYLMESLRITNENLIPVLTVNLFFGLI
                     AFFTYFTLMFLVFRISYERFYQNILLGYLGIPAVTYFLCGLLRFYLSLVRKSKINLEL
                     WLIDFRKFLQMFFLFIIYYSLYVLYIKILDDFREFEGIIQIRVLLGAVVFAWIIVRMI
                     FAPFFVIDRGYTVRKALRSSLLLTSGRTVKTFFLLFAVSVPFFPVLAGLYLLFNGNYA
                     AHIIIISSVLFSIYILYFFSPALIALVLSFDIYLKNRFSRRRKLINEAAVDTKKILED
                     SGIFTVVNTGNSEEE"
     gene            1206..1751
                     /gene="lepB"
                     /locus_tag="PK683_13410"
     CDS             1206..1751
                     /gene="lepB"
                     /locus_tag="PK683_13410"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNH09491.1"
                     /translation="MYSYKKKSLLYDNLPAFRKLFIRLTVSLVLGIVLSFVFKSYIIF
                     AWKFNTDSMRPGTARGKTGFWNRMFSREKMNYGKIYLYKIQNAELVLPGRLAGKPGDV
                     LEIKSKKLRRNGEAVEDKSAFFSDPRPAFPQNISSRDFTEKISLGQNEFFFLCDNRDE
                     CMDSRTVGKVQKSDILGELIF"
BASE COUNT          470 a          361 c          361 g          574 t
ORIGIN      
        1 taagcaccgg tccttcgaag gaaaatacgg tgggatttgc cgtttcaatg gcagtctttg
       61 ttcttttgtt ctggacatcc ttccgggttc tgccttatca tactttattg actgaatata
      121 cttcagagat cagacagtcc gcaatgttcg gctgggtgcc ggaactgttt tatgcttccg
      181 ttgccctggt catactgtcc tttctctgga tggtgtactc cggtcttgtt ctgataagaa
      241 attcgaggta ggattatgtt attcaaactg ctgctccgtc tgctcacacc gccgaggatt
      301 tttttccgtt atctgatgga gtctctccga ataacaaatg aaaatctcat cccggtactg
      361 acagtgaatc tgtttttcgg cctgattgca ttttttacat actttactct gatgttcctg
      421 gtattcagaa tttcctatga aaggttttat cagaacatac ttctcggata cctcggaatt
      481 cctgcagtca catattttct ctgcggactg ctccgctttt atctttcgct cgtgagaaag
      541 tcaaaaatca atctggaact ctggctgatt gatttcagga agtttcttca gatgttcttt
      601 ttatttataa tatactattc attatatgtc ctatatatta aaattcttga tgattttcgg
      661 gaatttgagg ggatcatcca gatccgtgtc ctgcttggcg cagttgtatt tgcctggata
      721 atagtccgta tgatcttcgc tccttttttt gtgatagacc ggggatacac tgtccgaaag
      781 gctctccgtt catctcttct cctgacaagc ggaagaacag tgaaaacctt ttttctcctt
      841 tttgcagtgt ctgtgccttt ttttcctgta ctggcaggac tttacctgct cttcaacggg
      901 aactatgcag cacatatcat cattataagt tcagttcttt tttccatcta catactttat
      961 tttttctctc ctgcactcat cgcacttgtg ctgagttttg acatttatct gaagaacaga
     1021 ttctcgagaa gaagaaaact gatcaatgag gctgctgtcg atacaaagaa aatactcgaa
     1081 gattcaggaa tttttacagt agtgaacaca ggaaacagtg aagaagaatg aaaggtgtca
     1141 taaatattcc gccggagaat taaaaatctc ttgctctgcg cctcggcact gatgtacgtg
     1201 acatcatgta ttcctacaaa aagaaaagcc ttctgtacga caatctccct gcattcagaa
     1261 aactgttcat caggctgaca gtttcccttg ttcttgggat tgtactttcc tttgtattca
     1321 aatcttacat catttttgca tggaagttca atactgattc aatgagaccg ggcactgcca
     1381 gaggaaagac cggtttctgg aacaggatgt tcagcagaga aaaaatgaac tacggaaaaa
     1441 tatatctgta taaaattcag aatgcagaac ttgttctccc cggaagactt gcaggaaaac
     1501 cgggagatgt tctggaaata aaaagcaaaa aactcagaag aaacggggaa gctgtggaag
     1561 acaagtccgc ttttttctct gatccgagac ctgcctttcc tcagaatatc agttccaggg
     1621 attttactga aaaaataagc cttgggcaga atgaattttt tttcctctgt gacaatcggg
     1681 atgaatgtat ggattcaagg acggttggaa aggttcaaaa gtcggatatt ctgggagaac
     1741 taatttttta atgcccgtaa aaatcg
//
LOCUS       DAOQGJ010000902         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48196_length_1766_cov_2.700175, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000902 DAOQGJ010000000
VERSION     DAOQGJ010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48196_length_1766_cov_2.700175"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..459)
                     /locus_tag="PK683_13415"
     CDS             complement(118..459)
                     /locus_tag="PK683_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09492.1"
                     /translation="MKEIGILEYIKMKLIPDKFKKDDPEKFLQIQSDFSVSTEEKQDH
                     KYEETEEEKRIIEEKAKRNWEKFQKKNQNRKDSKPAVSLLRESLLLKAAVIFLSVGVS
                     LFIYKVFRKKT"
     gene            complement(472..1029)
                     /locus_tag="PK683_13420"
     CDS             complement(472..1029)
                     /locus_tag="PK683_13420"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH09493.1"
                     /translation="MPETNFPEPPADRWADWTGALSPGDEVYIITRDIYLDLFEKIFR
                     RARKYGKNHDDSEDIANEVLELLYKKLQEICESRTVIIKSLASLCWAWIHIYFNSIIA
                     RKRKDIPFLMENESYLTDIIEDLSECIRQLDEQSGRIITKYFFESKTLDEIAKEEGIS
                     LNKTYRIKESAERALYHCMKRKGYP"
     gene            1217..>1766
                     /locus_tag="PK683_13425"
     CDS             1217..>1766
                     /locus_tag="PK683_13425"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNH09494.1"
                     /translation="MLKKLRYVSISIIIIPIFLAASLHISGLLNSFESFLMDSRQVYS
                     NPKLHFSEKVIIVQIDNSTIQKFSWPMPRDSYADVLQHIASGKPSSILFNIFFTAKKK
                     EEKGDLEKITQEIPNLSHQVHLDISPSKDDINSIPHLEKFSVSHIEEAESVKIYNSAE
                     LPAAEILNAAHHIHTTQEDFDKD"
BASE COUNT          508 a          349 c          286 g          623 t
ORIGIN      
        1 tacattaggt tgatttccat cgagattaaa caatttttaa aatttgatga tttgtcatat
       61 aattttgcat tagttgaatt taataaattt tttaaattgt ttaacaatgt tgataattta
      121 tgtttttttt ctgaatactt tatagataaa aagtgataca ccgactgaaa ggaagattac
      181 ggcagccttc agtagaaggc tttccctcaa aagcgaaact gcaggcttgg aatctttccg
      241 attctgattc tttttctgaa acttttccca gtttctcttt gccttttctt cgattatcct
      301 tttttcttct tctgtttctt catatttatg atcctgcttc tcctccgttg aaacggagaa
      361 atcagactga atctgcagaa atttttcagg atcgtccttc ttaaatttat caggaatcaa
      421 tttcatcttt atatactcaa gaatgccgat ttctttcatc gaactcacct cctacggata
      481 tccctttctt ttcatacaat gatacaaggc tctttcggct gactccttga tccgatatgt
      541 tttgttcaga gagattcctt cttcctttgc tatctcatcc agagtcttag actcaaaaaa
      601 atacttagtg atgattctgc cgctctgttc atccagctgt cttatgcatt cgctcaaatc
      661 ttcgataata tcagtcagat atgattcatt ttccataaga aagggaatgt ccttccgttt
      721 cctcgcaata atgctgttga agtaaatatg aatccatgcc cagcaaagag aagccaggga
      781 ctttataatg actgttctgc tttcacagat ctcctgcagt tttttgtaga gaagttccaa
      841 tacctcattt gcaatgtcct cactgtcgtc gtgattcttt ccgtattttc tggcccttct
      901 gaatattttt tcaaaaagat ccagataaat atctctcgtt attatataga cctcatcacc
      961 gggacttaag gctccagtcc agtctgccca acggtctgca ggaggttctg gaaaatttgt
     1021 ttctggcatt ctgatttcct atgtttccat ataaagattg aaaaatcttc tttttttttg
     1081 aagttttttt cgcgagatga gatttttttg gggattttcg gaatacggga ttttggttta
     1141 ttacaataaa tcaaatactg cggaaaagag ttgcctattt ttttgaagat gaaatactga
     1201 tttgacaggc tgtcaaatgc tgaagaaatt aagatatgta tctatttcta ttataattat
     1261 tcctatcttt ctcgcagcct cattgcatat ttcaggcctg ctgaactctt ttgagtcatt
     1321 tttgatggac agcagacagg tctacagcaa tccaaaattg catttttctg aaaaagtcat
     1381 cattgttcaa attgacaatt ctacaattca aaaatttagc tggcctatgc ccagagacag
     1441 ttatgcagat gtactccagc atattgcatc cggaaaacct agcagcatat tgttcaatat
     1501 cttcttcaca gcaaaaaaaa aggaagaaaa gggagatctt gagaaaatta ctcaagaaat
     1561 tccaaacctg tcgcatcagg ttcacttgga tatcagtcca tccaaggatg atataaattc
     1621 tatcccgcat ctcgaaaaat tttccgtatc tcacattgaa gaagcagagt cagtcaaaat
     1681 ttataattct gcagaattac ctgctgccga aattctcaat gctgcccatc atattcatac
     1741 aacacaggaa gattttgaca aggacg
//
LOCUS       DAOQGJ010000903         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48224_length_1765_cov_3.667251, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000903 DAOQGJ010000000
VERSION     DAOQGJ010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48224_length_1765_cov_3.667251"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..>1765)
                     /locus_tag="PK683_13430"
     CDS             complement(211..>1765)
                     /locus_tag="PK683_13430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH09495.1"
                     /translation="KSMADFLTVEADCRMESSGMSIQRKKEMRESEVPLSIINNVHKT
                     GRMIVLENASASGDFVNDPYVKSQSSKSILCFPVENRNKLLGIIYLENSLAAYVFSES
                     RVEILNILSLQIAISLQNAKFITDIEEARKKAEEANAVKSDFLANMSHEIKTPMNGIL
                     GMKSLLEMTDLNLEQKEYLRVIGICSESLLIIINDILNISKLESGSISLEELPFDLSR
                     SAADTVQIVQNEIRQKKLYLDFHIDNDVPQFIIGDESRLRQILLNLLNNAVKFTNTGG
                     IDFSIRLRGRAENRAYIEFSVKDTGIGIEEEKLSKIFDPFTQADSSISRKFGGTGLGL
                     AISRKLAELLGGSITAESAFGKGSNFILSLNAEEYQISDKSRTENPEVDFREPKQNDF
                     PSILLIEENIIDKNLIIKLLKNIGYRIKNSVLNSAEAEKKLRFGNYDLILMDMEKNDA
                     GADALRSFQEIFHEKNSPVIIGFVNGINEEVKNMYVKAGVSDFISKPIDINEMKHKIL
                     YWTSSIKKQ"
BASE COUNT          501 a          390 c          307 g          567 t
ORIGIN      
        1 gacattttgc aatgactttt tcttaaacaa ttccaaagag ggcagataaa gacataaaaa
       61 atagacaata tattcatcgt aactgattgt aaaaaagctt atatagaaaa gcttgaatgg
      121 catattttgg gcagcttcca gtggagcggc ttcaagcacg tgccaaggcc ggaaaaacag
      181 tccccgatct gaaaaccggc ggcagaacag ctactgcttt tttattgaag aagtccaata
      241 caagatcttg tgcttcattt catttatatc gatcggcttg ctgataaagt cgcttacacc
      301 tgccttgaca tacatattct taacttcctc attgattcca ttgacaaagc cgattataac
      361 cggcgaattc ttttcatgga atatttcctg aaatgatctc agtgcatctg ccccagcatc
      421 attcttttcc atatccatca gaatcagatc ataatttccg aacctcagct tcttttctgc
      481 ctccgcagaa ttcagaacgg aattttttat ccgatatccg atgtttttta gaagttttat
      541 aattaaattc ttatcaataa tattctcttc aatcagaagg atggaaggaa aatcattctg
      601 tttcggttct ctgaaatcga cctcaggatt ttcagttctt gatttgtccg atatctgata
      661 ttcctcagca ttgagactca ggataaaatt gcttccttta ccgaatgcag attctgcagt
      721 aatgctcccg cccagaagtt cagcaagttt tctggagata gcaagcccaa gacctgttcc
      781 tccgaatttc ctggaaattg aactgtcagc ctgagtaaac ggatcgaaaa tttttgaaag
      841 tttctcttcc tcaatgccaa ttcccgtatc ctttacggaa aattcgatat atgctctgtt
      901 ctctgccctg cctctcagtc tgatgctgaa atcaatcccc cccgtattcg taaactttac
      961 agcattgttc agaagattca gaaggatctg tctcagacgg gattcatcac cgataatgaa
     1021 ctgcggcaca tcgttgtcaa tatgaaagtc caaatagagt tttttctgcc gaatttcatt
     1081 ttgcacaatc tgaacagtat ctgcggcaga tcttgaaaga tcaaacggaa gctcttccag
     1141 agaaatactc ccggattcca gcttcgaaat attcagtata tcatttataa ttataagaag
     1201 actttctgaa cagatgccta tgactctcag atattccttc tgctcgagat tcagatcagt
     1261 catttcgaga agagacttca ttcctaatat tccgttcata ggtgttttta tttcatgact
     1321 catatttgcc aggaaatctg acttgaccgc atttgcctct tctgcctttt ttcttgcctc
     1381 ttcaatatct gtaatgaatt ttgcattttg aagggatatg gcaatctgaa gagagagtat
     1441 gttcaggatt tccactctgc tttcagaaaa aacgtaagca gccagactgt tttcgaggta
     1501 tattattccg agaagtttat tcctgttttc cacaggaaaa cagagaattg attttgaact
     1561 ttgcgacttg acgtaaggat cattcacaaa atctcctgaa gcagaggcat tctcgagaac
     1621 aatcattctt ccagtcttgt gcacattatt tatgattgac agcggaactt cagattcccg
     1681 catctctttc tttctctgaa tgctcatacc gctgctttcc atcctgcagt ccgcttcaac
     1741 agtaagaaaa tcagccatgc tcttt
//
LOCUS       DAOQGJ010000904         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48245_length_1765_cov_2.539181, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000904 DAOQGJ010000000
VERSION     DAOQGJ010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48245_length_1765_cov_2.539181"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PK683_13435"
     CDS             complement(<1..669)
                     /locus_tag="PK683_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH09496.1"
                     /translation="MSNLENKTVIVTGGGSGLGRSVCIEYARQKANVVFCGRREKEGN
                     ETLEVLKTIHSKAVFVKTDITNEKDVQSLVWTALDRFGSVDILVNNAAVSGKKGRIAD
                     LSLDEFQEIVDINLKGAWLCMKYCIPEMLKKGKGSIVNVSSAAGMVGVAFGAGIYSAT
                     KHALVGLSKSGALEYGAKGIRVNAVCPGTFQTEMLDWIFNNSPDPEKARQKIIDANPV
                     KRIAG"
     gene            792..>1765
                     /locus_tag="PK683_13440"
     CDS             792..>1765
                     /locus_tag="PK683_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HNH09497.1"
                     /translation="MKLMLKSITILSILLLISCTFKKKTAIKFLIPLTSSTSANSSVS
                     GALTSLVPSAGSLSPSFSQTVYSYSITVGSNVSSIQFTPTASDSSVSITINGTSAASG
                     SLSSPVSLISGTNTVTVIAAAVTYTITVTRTATSANLTSLTASPMNFYPSFSSSTSAY
                     TATVSSSISSITLTPVLSDSSASVTVNGTAVPSGSASGAVSISNGNNTIAVTVTAADG
                     SVTNYSLAVFRLSASQKRLFVTASATNGNLGGIAGADSLCGTDANKPTDGGTYKAVIV
                     DALLSRRACSNANCTLISENINWVLGANLTYLRPTDGTAIFTTNSAGI"
BASE COUNT          471 a          457 c          323 g          514 t
ORIGIN      
        1 acctgcaatc ctcttcacag gattggcatc aatgattttc tgtctggctt tttcaggatc
       61 agggctgtta ttgaaaatcc agtccagcat ctccgtctga aaagtaccag gacagactgc
      121 attgactcgg atgcccttgg caccgtattc gagagcgcct gatttggaaa gtcccaccag
      181 tgcatgtttt gtggctgaat aaattcctgc accgaacgct acaccaacca ttcctgctgc
      241 agaggaaaca ttgacaatgc tccccttccc ttttttcagc atttccggaa tgcagtattt
      301 catacagagc caggctccct tcagattgat gtctactatc tcctgaaatt catccaaact
      361 caaatcagca attctgccct tctttcctga tacggctgca ttattgacca ggatatctac
      421 tgatccaaat ctatccaatg ctgtccagac aagggactgg acatcctttt catttgtaat
      481 gtcagtcttg acaaatacgg cttttgagtg gattgtcttg agcacttcaa gggtttcatt
      541 tccttccttc tctctccggc cgcaaaaaac cacatttgct ttctgtctgg catattctat
      601 gcagacagaa cgtccaaggc cggaaccgcc tcccgtaaca atgactgttt tattttctaa
      661 attagacata cttctaaaat tgagataaaa taaaaaaagg aaagcttttt tgaataaaaa
      721 aacagagaaa atatataaaa aacttgctct tctacttata atacaatata actggaggca
      781 ggatcaatga aatgaaactg atgctcaaat ccattacaat cctgtctatt ttattgctga
      841 tttcctgcac cttcaaaaag aagactgcca tcaagttctt aatccctctg acgtcttcca
      901 cttcagcgaa ttcctctgtt tcaggtgcac tgacttccct cgttccttcc gcaggttctc
      961 tttcaccatc cttttctcag acggtttaca gttattcaat cactgtagga tcaaatgtga
     1021 gttccattca gttcactcct acggcttccg acagttcagt ttccattacc atcaacggaa
     1081 catctgctgc ttccggcagt ctgtccagtc ctgtttctct gatatccgga accaacacag
     1141 tcacagtgat cgctgccgct gtcacttata caattacagt cacaagaact gccacttctg
     1201 caaacctgac ttcgcttacc gcctcgccaa tgaactttta ccccagcttc tcttcatcga
     1261 catcagcata cactgcgacc gtttccagtt caatcagttc aattactctt acacctgttc
     1321 tttccgattc atctgcatct gtgacagtaa acggtacggc cgttccttcc ggatcggcat
     1381 ccggtgctgt cagtatttcc aacggaaata ataccattgc cgttacggta acggctgcag
     1441 acggttctgt caccaattat tcattggcag tcttcagact gagtgcctcg caaaaaagac
     1501 tttttgtcac tgcatcagca acaaatggaa atttgggagg aattgctgga gccgacagcc
     1561 tctgcggtac ggatgccaat aaaccgacag acggcggcac ttacaaggct gtaattgtgg
     1621 atgctctcct cagcagaaga gcctgttcaa atgcaaactg tactctgatt tctgaaaata
     1681 tcaactgggt tcttggagca aatctgacct atctgagacc gacagacgga acagcgatat
     1741 ttacgacaaa cagtgcaggt atttt
//
LOCUS       DAOQGJ010000905         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48275_length_1764_cov_3.043885, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000905 DAOQGJ010000000
VERSION     DAOQGJ010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48275_length_1764_cov_3.043885"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PK683_13445"
     CDS             complement(<1..495)
                     /locus_tag="PK683_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09498.1"
                     /translation="MKARNRLNPIQDSSELEEFLDFDFTLEDLAELNEELGLSDSGEL
                     LMDFDDEEDSHLDEIVDRSVGNSDDEDHLLGARVPDIYINEDKDDLSEDDLEDILSTP
                     LVGQKSTFDKFDADVDSDISLSTEELLKIESEDPYAFVDSDTNIDRHFADQISDRDDS
                     DITLD"
     gene            complement(538..801)
                     /locus_tag="PK683_13450"
     CDS             complement(538..801)
                     /locus_tag="PK683_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09499.1"
                     /translation="MTLCISVATWVYFKDRRDLLKKIYMPGTVTVFLQGNVSKSGIYN
                     IPAGADLQDLLELSGNADSVNPKEIQMESSLFDGQIVRIGMGK"
     gene            1195..1764
                     /locus_tag="PK683_13455"
                     /pseudo
     CDS             1195..1764
                     /locus_tag="PK683_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
BASE COUNT          527 a          394 c          357 g          486 t
ORIGIN      
        1 gtccagagta atgtcagaat catccctgtc agaaatctga tcggcaaaat gacggtctat
       61 gttagtatca gaatccacaa atgcgtacgg atcttccgat tctattttta aaagttcttc
      121 tgtggataga gagatatcag aatccacgtc tgcatcaaat ttatcgaaag tggatttttg
      181 tccaactaat ggagtgctca aaatgtcttc cagatcatct tcggaaagat catccttgtc
      241 ttcattaata tatatgtcag gaacccgggc tcccagaaga tgatcttcgt catcggaatt
      301 gccgacggaa cggtctacaa tttcatccag atgtgaatct tcttcgtcat caaaatccat
      361 cagaagctca cccgaatcgg aaagacccag ctcctcattc agttcagcaa gatcttccaa
      421 ggtaaaatca aaatcaagaa attcttccag ctcactgctg tcctgaatgg gattcaatct
      481 gttccttgct ttcatctcag gccctgtttt ttgcgaaatt ttcccttgat cagtcattta
      541 cttccccatt ccaatcctga ctatttggcc atcaaaaaga ctcgattcca tctgtatttc
      601 ttttggattc actgaatctg cgtttccaga caattccaga agatcctgaa ggtcagcacc
      661 tgccggaatg ttataaatgc cggacttgga gacatttccc tgcagaaata cagtaacagt
      721 tccaggcata taaatcttct tcagcagatc gcgtctgtcc ttaaaataga cccatgttgc
      781 aacgctaata caaagagtca aaaccacaac tagttttttc gtttccaaaa gaatgcctca
      841 gattaatttt cggaaagcct gatccaaaac tcaagatttt cagataagtc atttattttt
      901 gacaattttc ggtcaatgta aaatgactgc tttcttccgc tttccgtttt tttcttgctc
      961 tgcagcagcc ttggctgtct gcgagagaat cttttacttt tttgatggat ttttcaggat
     1021 tgtaattatt cagaatccat aaatcatgag cgaaaaacaa ggcaggacac attgagaaaa
     1081 atcagacttt ttttttcagc agcggttgcg gcagcggcgg ctttagggtg cgggggcccc
     1141 tacgttaaaa ttccggacag tgcagaactc ggcaaagaat attcagcagg cagagacgat
     1201 gagatcgcta aaaggactct tgaactgaca ctgaattctc tgaaaaaaaa gaaggcagac
     1261 agcccggatt ccaaagttct ccgtgacgct catccgaaac atcacggatg cactgaggcc
     1321 tcattcactg tagaaaagaa catcccggat gcactgaagc acggaatatt taaaaatcct
     1381 aaaacataca gtgcatatat ccgtttttcc aacggatctc agactcctca ggcggacagc
     1441 atcggggaca tcagaggaat gggaatcaaa ctgatgggcg tagagggaga caagcttctc
     1501 gaatccgaaa aaaaagagaa gacccaggat tttctcatta tcaaccaccc tgtacttccg
     1561 gtaggagatc cggaggaata tttaaacctg tttgaagctg catttgcagg aaaggcagga
     1621 agttattttt tcggatggaa tccgttcgga tggaaacttg caggacttgg aaaagtcagg
     1681 gcgatcagat caaaaaaaat ctcatctatg cttgaaatca gatactggag cactactccc
     1741 tatcagctcg gtcagaacgc agtg
//
LOCUS       DAOQGJ010000906         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48333_length_1763_cov_3.401639, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000906 DAOQGJ010000000
VERSION     DAOQGJ010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48333_length_1763_cov_3.401639"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK683_13460"
     CDS             complement(<1..369)
                     /locus_tag="PK683_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09500.1"
                     /translation="MTESVYKFFYKKLYIFSEKSAELFFVFFFSSCISCTQAESRDRL
                     LDQTVESYFKVWSKGDMEGYSSIFHKSAVIQYKSRTGQMITEQLSDFIAGQRNAHRLN
                     PGKMNEIPLEKRYHQNGDTAQ"
     gene            complement(366..1565)
                     /locus_tag="PK683_13465"
     CDS             complement(366..1565)
                     /locus_tag="PK683_13465"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNH09501.1"
                     /translation="MTGKIEDAIKEIQNGRMIILVDSEDRENEGDLVIAAEFADKDAI
                     NFMATYGKGLICIPMTKEKLTLLGLSRMVEKGGDHHGTAFTVSVDAKDGVSTGISAYD
                     RAKTISVLLDDKSRPDDLVRPGHLFPLEAVKGGVLRRAGHTEASVDLCVLAGLKPAGV
                     ICEIMNEDGTMARMNDLEKFAEKHGLNIYTIADLIAYRTQKDQLIRMEAEARLPTEYG
                     EFTIKAYSTIIDDKIHIALIKGEIHPEDTVIVRVHSECLTGDTFFSSRCDCGPQLHAA
                     LAKIEKEGKGVLLYMRQEGRGIGIINKLKAYGIQDTGLDTVEANEKLGFPPDLRDYGI
                     GAQILKNLGVENMKLMTNNPRKIVGLEGYGLKVQGRIPLEINANNENEKYLKTKKLKL
                     GHLLHGV"
BASE COUNT          409 a          416 c          363 g          575 t
ORIGIN      
        1 ctgggcagtg tctccgttct gatgatatct tttttcgaga ggtatctcgt tcatctttcc
       61 cggattcagt ctgtgtgcat ttctctgtcc ggctataaaa tcggaaagct gttctgtgat
      121 catctggccg gttctcgact tgtactgaat gactgcagat ttatggaata tggaagaata
      181 gccctccata tctccttttg accagacttt gaaataggat tccactgtct gatcgagaag
      241 cctgtccctg ctttcagcct gggtgcagga gatacaggaa gaaaaaaaga aaacaaagaa
      301 cagctctgcc gatttttctg aaaatatata taatttttta taaaaaaatt tatatacaga
      361 ctctgtcata cgccgtgaag aaggtgtccg agtttcagtt tctttgtctt caaatatttc
      421 tcattttcat tattggcgtt gatttcaagg ggaattcttc cctgtacttt caggccgtat
      481 ccttccagac cgacaatctt tctgggattg tttgtcatca gtttcatatt ctcaacaccg
      541 agatttttca gaatctgtgc accgatcccg taatctctca gatcaggagg gaaaccgagt
      601 ttttcgttcg cttccactgt atcaagtcct gtgtcctgaa tgccgtatgc cttcagcttg
      661 ttgatgattc cgattcctct tccttcctgt ctcatataaa gaagtacacc tttgccttct
      721 ttttctatct tggcgagagc tgcatgaagc tgaggcccgc agtcgcatcg gctgctgaaa
      781 aaagtgtctc cagtaagaca ttcggaatga actcttacaa tgactgtgtc ttcaggatga
      841 atttctccct tgattaatgc tatatggatt ttatcatcaa taattgtaga atatgctttt
      901 atagtgaatt cgccgtactc agtgggaagc ctggcctcag cttccatccg tatgagctga
      961 tctttctggg tccggtaggc aatcagatct gcgattgtat agatgttaag cccgtgtttt
     1021 tcggcaaatt tttccagatc gttcattctg gccattgtac cgtcttcgtt cataatttca
     1081 catatgactc ctgcaggctt cagtcccgcc agaacacaga gatcgacaga tgcctcagta
     1141 tgaccggccc ttcggagaac cccgcccttg accgcttcca gagggaaaag atggcctggt
     1201 ctcaccaggt catccggtct ggatttatca tccagaagca ctgaaattgt ttttgccctg
     1261 tcataggcgg aaatgccggt gctgaccccg tctttggcat ccacggaaac agtgaaggcc
     1321 gtgccatgat ggtctccgcc tttttctacc attctgctca gtccaagaag ggtcagtttt
     1381 tccttagtca tcggaatgca gataagcccc tttccgtacg tggccatgaa attgattgca
     1441 tccttatctg cgaactctgc agcaatgacc agatcgccct cattttctct gtcttcagag
     1501 tctaccagga taatcattct gccgttttgt atttctttga ttgcatcttc aattttacca
     1561 gtcatgtttt taccatacca gaaaatgggt tcagagtctc cattcttatt tagcccgaat
     1621 tctttgcact gtcactcagg tcatattttc ttcctcttta tgctttccgc agataccttg
     1681 aagaatgcaa ttgtctgatc aagtgtcgca gcatgtttgt tcagaagttc agagtttgct
     1741 gcaagttctt ctgaggatgc agc
//
LOCUS       DAOQGJ010000907         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48375_length_1762_cov_3.574107, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000907 DAOQGJ010000000
VERSION     DAOQGJ010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48375_length_1762_cov_3.574107"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..>1762)
                     /locus_tag="PK683_13470"
     CDS             complement(55..>1762)
                     /locus_tag="PK683_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09502.1"
                     /translation="SLFCILPAVTFCGFKKPPKYILSLQTSSYPSGTPGITAGNPDRN
                     TNEFGATAVFSAVLNSEPISNIQICISSSDELQGGTILNTGSVSAPDGTCGSGISYIS
                     FSPSSWNIPLQISVKGSRGSVGSKGNTDYKIILKAQGSDSRFSSLAAASVKLKNLDID
                     DTNSFFIRTNLTGLNSGTLVLKLNGTDSLSLNANGTDSFPNPISSGSSYYIEIKNQPS
                     GHTCAFTDSPYGIINSHIIVNIECVSGYLFNGSIFSTSSPPTLSQSFAGIETISGSFP
                     PVSSAGNADGPGSSARFDNPIAIAADGVNLYAADIFNNLIRRIKISDGTVSTAASVSG
                     PHGIATDGSNLFAASYNGHVIYKIVLSTGAVSVLSGTGVSGDAVGASSSAQFNTPVYL
                     TTDGTYLYVSDRGNAKIKQIKISDGTVSTFASGLIFPNGLATDNTYVYAADSGAHQIK
                     RYKISDGSITVIAGTGAAGGSDNASGPSATLNEPYGITVDGSFIYTLEGAGKRLRKIS
                     VNSPYSVTTIVPQNDGYLDGIIGSAQFCGAGLYCDSSLTTDGVSLFFADRFNNSIRKL
                     YY"
BASE COUNT          496 a          360 c          400 g          506 t
ORIGIN      
        1 tgccttctga aaattacaga atctagatag catattgtat ttgcgtaagg tgagtcagta
       61 atacagtttt cttatggaat tgttgaaacg gtctgcaaag aaaagggaaa ccccgtcagt
      121 tgtcaatgat gagtcacagt ataaacctgc accgcagaac tgcgcagatc cgataattcc
      181 gtccagataa ccgtcattct gaggaacaat cgtagttact gaatagggag agtttacact
      241 gatcttacgc agccttttcc ctgctccctc cagtgtataa atgaatgacc cgtcgacagt
      301 gataccgtac ggttcattca gagtcgccga cggtccggat gcattgtctg atccgcctgc
      361 ggctcctgtc cctgcaatca cggttattga tccgtctgat attttatatc gtttaatctg
      421 atgtgctcct gaatcggcag cataaacata agtattgtcg gttgcaagtc catttggaaa
      481 aatcagccct gatgcgaatg tcgaaacagt cccatctgat atttttatct gctttatttt
      541 tgcattgcct ctgtccgata cgtacagata tgtaccgtca gtcgtaagat agaccggagt
      601 attaaactgt gcactcgacg aagcgcctac tgcatcaccg gaaactcctg tgcctgagag
      661 aacggaaaca gcacctgtag aaagcactat tttatagata acgtgaccat tgtagcttgc
      721 tgcaaaaaga ttgcttccgt ctgtagcaat gccgtgagga ccggatactg atgcggccgt
      781 agatacagtt ccgtctgaaa tttttattct gcgtataaga ttgttgaata tatctgcagc
      841 atagagattt acgccgtctg cagcgatggc aataggattg tcgaatcttg ctgatgaccc
      901 aggtccatct gcatttcctg cagaagatac aggcggaaaa gaacctgaaa ttgtctctat
      961 tcctgcaaat gactgagaaa gtgtgggagg agatgaagtg gaaaaaatag atccgttgaa
     1021 caggtaaccg cttacgcatt ctatattgac tataatatgt gaatttatta ttccgtacgg
     1081 cgagtcagtg aaggcacaag tatgacctga aggctgattc ttaatctcga tataatagga
     1141 acttccggaa ctgatcggat tgggaaagct gtcagttcca ttggcattta aagacagtga
     1201 gtcagttccg ttcagtttca gcacaagagt tcctgaattt aaaccggtga gattagttct
     1261 tataaaaaag gaatttgtat cgtctatatc caggtttttc aatttcactg aagcggctgc
     1321 aagactgctg aatctggaat ctgatccctg tgctttaagt atgattttat aatcagtatt
     1381 tccctttgat ccgacgcttc ctctgcttcc tttcactgaa atttgaagag gtatattcca
     1441 gctggaagga ctgaagctga tataagaaat gcctgatccg caggttccat ccggggcaga
     1501 aacactgccg gtattcagta ttgttcctcc ttgaagttca tctgaactgg aaatgcatat
     1561 ctgaatattg gaaatcggtt ctgaattaag tactgcggaa aatacggctg tcgctccaaa
     1621 ctcatttgta ttccggtcgg gatttccagc ggtgattcca ggagtgcctg aagggtatga
     1681 cgatgtctga agtgaaagga tatacttggg cggtttttta aacccgcaaa aagtaactgc
     1741 aggaagaata caaaacaatg aa
//
LOCUS       DAOQGJ010000908         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48429_length_1761_cov_3.484760, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000908 DAOQGJ010000000
VERSION     DAOQGJ010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48429_length_1761_cov_3.484760"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PK683_13475"
     CDS             complement(<1..698)
                     /locus_tag="PK683_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09503.1"
                     /translation="MMKFLSAVLSVIVLNCSNSEEGLFSEKNMLFLRAGTASLSGKVM
                     KGAVKNAAVSVYALNKDGTCSTNSGPIAAASTDSDGNYKAYFPKTGRPVCVIATPKSD
                     SKMYDEASKADISWTGAVSMTMIIKEPIGGASKTGLNLTPLSRFAAARLQSIAKNNST
                     GSRLDEQVTYANKQIAVQFGLHKGFAKKNALTRADSSSIDLDSIPDILDEGIKLDIGT
                     DSNAKYVKALLGGFS"
     gene            1343..>1761
                     /locus_tag="PK683_13480"
     CDS             1343..>1761
                     /locus_tag="PK683_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09504.1"
                     /translation="MPQSYSSTKEGNLILRIEDDDLTIPDILISASEHPPKLYLTSSY
                     SPSVFFLLPLKSSGYFFPTVQKNLKLELKTDLQTNRDYSFFLPSGNYYMNITNHSDAG
                     LDFPVFLDRNIPKQMNLSFGFRVKTETEYQYSVENYSG"
BASE COUNT          516 a          374 c          314 g          557 t
ORIGIN      
        1 gagaatcctc ccagcaaagc tttcacatac tttgcattgg agtctgtgcc gatgtcgagt
       61 ttgatgcctt catccaaaat atcagggatg gaatctaagt cgatagaaga ggaatctgcc
      121 cgagtaagtg cattcttctt tgcaaatccc ttgtgcagtc cgaactgaac tgcaatctgc
      181 ttgtttgcat acgtgacctg ctcatccagc ctcgaccctg tactgttatt ctttgcaatg
      241 gactgcagtc tggctgctgc aaaccggctg agaggagtca gattcagtcc ggtcttggat
      301 gcgcccccga tcggttcttt tataatcata gtcatagaga ctgcaccagt ccagctgatg
      361 tctgccttgc ttgcctcgtc gtacatcttc gagtctgact taggcgtggc gatgacgcag
      421 acaggtctgc ctgttttggg aaagtaggct ttgtagtttc cgtcggagtc tgtacttgca
      481 gctgcaatcg gacctgaatt tgtactgcag gttccgtcct tattgagtgc atagacgctt
      541 actgcagcat ttttgaccgc acctttcatc actttgccgc tgagagaagc agttcctgcc
      601 cggagaaaaa gcatattttt ttcagaaaat aatccttcct ctgaatttga acagttcagc
      661 acaatgactg aaagaacggc acttaagaac ttcatcacaa aaccctctat atatctatct
      721 ggttgaattc tactgcataa aacatcctca ttcaaacatt ttttacttta taatatactt
      781 ttttatataa gtgtttacac ctattttctg agatatttat tcattgtgat tttgataatt
      841 acaatcagtt agaaaataga tcttgcaaag tcctctggat attatatact ataattacac
      901 atacagttct gtcccgaaac tgatttcatt tttacttcga tatccttcaa tctgaaaatc
      961 tgagctatga tctttaagag gaaaggatat aaatacgtcc tcacagacag gaaaaatccc
     1021 agtattaaac cctgtctaat gacagattgc ggactttctt aaaattcaat atgcaattga
     1081 caaattcaat ttctgctctt acagactcac cttacgcaaa tacaatatgc tatctagatt
     1141 ctgtaatttt cagaaggtat taggaaaatc taatgaatca gaaaaaaagg gatttttaag
     1201 ggcaaagccc ttaaaaccgc cggtaggctc ctgccaaaaa cgccccacct gcgtaaggtg
     1261 agttacagat atatttatgc ctataatcta tatcataatc tctattttat ggaattgttc
     1321 attttttcag gataaaagac ctttgcccca aagctacagt tcgacaaaag aaggaaacct
     1381 tatacttaga attgaagacg acgatctgac aatccctgat attttaattt cagccagcga
     1441 gcatcctccg aaactttatc tgacatcatc ttattctcct tctgttttct ttctgcttcc
     1501 attgaaatca tcagggtatt tctttcccac cgtgcagaaa aatttaaagc tggaactgaa
     1561 gactgatctt cagacaaaca gagactattc tttttttctc ccttcaggaa attactatat
     1621 gaatataaca aatcactcag atgcaggact cgattttcct gtatttcttg acaggaatat
     1681 tccgaaacag atgaatttaa gttttggatt tagagtaaag acggaaacag aatatcagta
     1741 ttctgttgaa aactattcag g
//
LOCUS       DAOQGJ010000909         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48436_length_1761_cov_3.161782, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000909 DAOQGJ010000000
VERSION     DAOQGJ010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48436_length_1761_cov_3.161782"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PK683_13485"
     CDS             complement(<1..800)
                     /locus_tag="PK683_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNH09505.1"
                     /translation="MKKSTLYLFFSLFLITGCLKTKKASFDVSSPAGFFFFGGIVSAI
                     AAANSTASFSIPDPNLTFSNVQNTSVTVTWKSASAVSGTITYKAYFSLISDMDTVANM
                     ESNGSFTQDYAASVNQMTFSGLIPNKYYYFNVAAQNSGTKLNYTKKCIQVYQDSTVTD
                     CSFDPLSVKGTAATVIGTCGTTGTTNGSFSAAQFKDPLYMQTVGNNIFLTEAHMNSGS
                     CAIRKLDMTNSLVSVYAGNIGSCSTYTDSTLTSSYFRSPHGIAFDGTNL"
     gene            1119..>1761
                     /locus_tag="PK683_13490"
     CDS             1119..>1761
                     /locus_tag="PK683_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09506.1"
                     /translation="MDKERPPQGFLPDASALQLSFHKHSIQAVFDFGRNHRYFLIFPF
                     YPPVKNPILNSRCADRKNAGLISSGKLPMISTMKFCISAVLFLLLSVCSVLFSETFFF
                     PGGKLEGEKGVWTETKTGTDPNELLESWRDEDWILLYDAKRKIYLNVPVKGGQSFQSK
                     EEKKNLWKPYVLLKKNKDKNNDKIITPENRDKKAKDPNTCLNAEEAKLVRLLTE"
BASE COUNT          541 a          359 c          368 g          493 t
ORIGIN      
        1 agatttgttc cgtcaaatgc tattccgtgc ggtgacctga aataagatga ggtcagtgta
       61 ctgtcagtat atgtactgca tgacccgata ttgcctgcat agacagatac cagactgttt
      121 gtcatatcca attttctgat tgcacagctt ccactgttca tatgggcttc ggtcagaaat
      181 atattatttc caacagtctg catatacaga ggatctttaa actgagccgc cgagaatgat
      241 ccgtttgtgg ttccggttgt cccgcaggtt ccgatgactg tggctgcagt tcctttcaca
      301 gaaagagggt caaagctgca gtcagttact gtagaatcct gatatacctg aatgcatttt
      361 tttgtatagt tcagttttgt tcctgaattc tgagcagcaa cgttgaagta atagtactta
      421 ttcgggatca gtccgctgaa tgtcatctga ttcactgagg ctgcatagtc ctgagtgaat
      481 gatccgtttg attccatatt agccactgtg tccatatctg aaatcagaga aaaatatgcc
      541 ttgtaagtga ttgtccccga aacagctgat gcagatttcc aggtgacagt cactgaagta
      601 ttctgtacat tggaaaaagt aagatttgga tccgggatac tgaaagaagc tgtcgaattc
      661 gccgcagcaa tcgcactgac aattccgcca aagaaaaaaa atccggccgg gctggacaca
      721 tcaaaggaag cctttttagt tttcagacag ccagtgatca gaaacaatga aaagaacagg
      781 tacagggttg attttttcat aatatatata acatctcagt ctatacgggt tttcaataaa
      841 tcgaaggaat tccttcataa aaaagcagat tcgaacagaa tacatctgaa tattatcatt
      901 cgcttttcac tgaggcacag acagaaccga agacgaataa ggagctcaac ttgttacatt
      961 ccgcaattct ggattccagg ttcagagcag tcacagctcc acagctgttc aagtgcgctt
     1021 tcggtcaatg aataaattaa aatttaatta taaatgtgaa tattatattt tcgaatttct
     1081 ccgatattgg catatatctt ttttcagagg aatagacaat ggataaggaa aggccgccgc
     1141 agggttttct gcctgatgca tctgcattgc agctctcatt ccataagcac tctattcagg
     1201 ctgtttttga cttcggccga aatcacagat actttctgat attccctttc tatccccctg
     1261 taaaaaatcc gatcttaaac agccgatgcg ctgacagaaa aaatgccggt ctcataagtt
     1321 ccggaaaact gccgatgata agtacaatga aattctgcat ctctgctgtc ctttttctgc
     1381 tcctatccgt ctgttccgtc ctgttttcag aaactttctt ttttcccgga ggaaaactgg
     1441 aaggagaaaa aggagtctgg acagaaacta aaaccggaac tgatccgaat gaacttctgg
     1501 aatcctggag agatgaggac tggatacttc tttatgatgc aaaaagaaaa atctatctga
     1561 acgttccagt caaaggcggt cagtcctttc agtccaagga agaaaagaaa aatctctgga
     1621 aaccgtatgt tctgcttaag aagaataagg ataaaaataa tgataaaatc attacaccgg
     1681 aaaacaggga caagaaagca aaggacccga atacatgcct gaatgcggaa gaggcaaaac
     1741 tggtcaggct tctgactgaa c
//
LOCUS       DAOQGJ010000910         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48490_length_1760_cov_2.870381, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000910 DAOQGJ010000000
VERSION     DAOQGJ010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48490_length_1760_cov_2.870381"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..>1760
                     /locus_tag="PK683_13495"
     CDS             99..>1760
                     /locus_tag="PK683_13495"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNH09507.1"
                     /translation="MEVTTPPYVKNKALIDWVKETAALCKPDKIYWCDGSQEEYDRLC
                     NELVAAGTFKKLNPAKKPNSYLAFSDPSDVARVEDRTYINSRRKEDAGPTNNWVEPSE
                     MKATLNKLFDGCMKGRTMYVIPFSMGPLGSPIAHIGIEISDSAYVAVNMKIMTRMGQK
                     VYDVLGANGEFVKALHSVGAPLQAGQKDVTWPCNPTTKYICHFPEENMIISYGSGYGG
                     NALLGKKCFALRIASTMGRDQGWLAEHMLILGVESPKGEKTYVSAAFPSACGKTNFAM
                     LIPPKAMGGWKVTTVGDDIAWLKMDENGQLRAINPEAGYFGVAPGTNYDTNPNAMESL
                     QKNTIFTNVALTTDGDVWWEGLTKEVPDGMIDWTGKPYVKGSDKPAAHANARFTAPAF
                     QCPSIDAKWEDPAGVPIEAIIFGGRRATTVPLVYQSFNWNFGVYLAATMGSETTAAAF
                     GQQGVVRRDPFAMLPFMGYHAADYFNHWLNFGRKLPNPPRIFGVNWFRKDANGKFIWP
                     GFGDNMRVLKWVVDRVHGRAFGVESPIGWVPKYEDLDWSGIDFPRE"
BASE COUNT          518 a          363 c          429 g          450 t
ORIGIN      
        1 aacttgatac agttcctgtc ggatttttga gcgaaaggag caaaaggaca gtcatctgct
       61 tttgactgtc aataataatt gtctggagat aaataagaat ggaagttaca acccctccct
      121 atgtaaaaaa taaggctctc attgactggg taaaagagac tgctgcactc tgcaagccag
      181 ataaaattta ctggtgtgac ggctctcagg aagaatatga cagactttgc aatgaattgg
      241 ttgctgctgg cacttttaaa aagctgaatc cagctaaaaa accgaacagt tatctcgcat
      301 tctccgatcc ttccgatgtg gcaagggtgg aagacagaac ttatattaat tccagaagaa
      361 aagaagatgc cggccccaca aacaactggg tagagccttc agaaatgaaa gctactctga
      421 acaaactttt tgacggctgt atgaaaggca gaacaatgta tgtcatccct ttcagtatgg
      481 gacctctcgg ttctcctatt gctcatatcg ggatcgagat ttctgattct gcttatgttg
      541 cagtgaatat gaaaattatg accagaatgg gtcagaaagt gtatgatgtg cttggtgcta
      601 acggtgaatt cgtaaaagcc cttcactctg tgggagctcc acttcaggca ggtcagaaag
      661 atgtgacatg gccttgcaat cctactacaa aatatatctg ccatttccct gaagaaaata
      721 tgatcatatc ttatgggtca ggatacggcg gaaacgctct tctcggaaag aaatgttttg
      781 ctctccgtat cgcttccaca atgggacgcg atcagggctg gcttgctgaa catatgctca
      841 ttctcggcgt ggaaagtcct aagggtgaaa aaacctatgt cagtgcagca tttccttctg
      901 cctgcggaaa aacgaacttt gcaatgctga ttcctccaaa agcaatggga ggatggaagg
      961 tgactactgt gggcgatgat attgcctggc tgaaaatgga tgaaaacgga cagctccgtg
     1021 caatcaatcc tgaagccgga tatttcggag tggcaccggg aacaaactat gatacaaacc
     1081 cgaatgcaat ggaatcatta caaaaaaata caatcttcac aaacgtcgca ctgacaacag
     1141 acggtgatgt atggtgggaa ggtctgacca aggaagttcc tgacggaatg attgactgga
     1201 ctggaaaacc ttatgtgaaa ggaagcgaca agcctgctgc acacgcaaat gcacgtttca
     1261 ctgcacctgc atttcagtgt ccttccattg atgcaaaatg ggaagatcct gcgggagttc
     1321 ctatcgaagc aatcattttc ggcggaagaa gagcaactac tgttccttta gtgtatcagt
     1381 cattcaactg gaatttcggt gtttatcttg ctgccacaat gggttctgaa acaacggctg
     1441 cagctttcgg tcagcaggga gttgtgagaa gagatccgtt tgcaatgctt ccttttatgg
     1501 gataccatgc agctgactac ttcaatcatt ggctgaattt cgggcgcaaa ctgccgaacc
     1561 ctccaagaat ctttggtgtc aactggttca gaaaagatgc aaacggaaag ttcatctggc
     1621 ctggattcgg agacaatatg cgcgttctga aatgggtcgt agacagagtt cacggaagag
     1681 cattcggagt ggaatctccg atcggatggg ttcctaagta cgaagatctg gattggagcg
     1741 gaattgattt cccaagagaa
//
LOCUS       DAOQGJ010000911         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48545_length_1759_cov_2.862089, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000911 DAOQGJ010000000
VERSION     DAOQGJ010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48545_length_1759_cov_2.862089"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PK683_13500"
     CDS             <1..807
                     /locus_tag="PK683_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNH09508.1"
                     /translation="FGHAGQDILSELMKSFGGFEHNKQSLRIVQKLENRYSDFPGLNL
                     CRMTLIGLLKHGAAYEDSDLLILRNENGPSLEALIVDYSDEIAYNIHDMEDGLESGIL
                     STETLSEIPLWDYFWKKGSAFERENGSELHTRKTLRSVMNAMVTDLIQNISDQIQEHK
                     IQSRENFSELWRNGKRIINYSEKMQSEILNMKRFLNEKLYQNPAVKNKSDYGKKIIER
                     LFYFFQKNFSEIPESYRERNTESRERIICDYIAGMTDRYAEKKTTEYGLN"
     gene            804..>1759
                     /locus_tag="PK683_13505"
     CDS             804..>1759
                     /locus_tag="PK683_13505"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNH09509.1"
                     /translation="MKLLIVESPAKIKTFQKVLGSEFIVSACFGHVADLPKGEFGIDI
                     EKDFQPEWKLLPKKSALIKKLKADAGRSESVYLATDPDREGEAIAWHLQRLLGKRKYF
                     RVRCTEITESGIRGAVHNLSELRTETVDSQISRRLADRIIGYRISPRLWDIRSGLSAG
                     RVQSVALKWIVHRERDIRKFIPEDYYEISLKTEVSGIPLLFRFVQKNNPSSAKLTEEE
                     KNRILSEFRLTEIQSGKDAHLMPPAEIKIISQNDKIIKKYPPPAFTTSSLQKEAYARF
                     RMPVAKTMRIAQKLYEGGTEKGGLITYMRTDSTRVSTERKNRA"
BASE COUNT          620 a          335 c          379 g          425 t
ORIGIN      
        1 ttcggtcatg ccggtcagga cattctttca gaacttatga agagtttcgg cggattcgaa
       61 cacaacaaac agtcgctccg cattgtgcag aaactggaaa acagatattc agattttcca
      121 ggactcaatc tatgcagaat gactctgata ggcctgctga aacacggagc cgcttatgaa
      181 gattcagacc ttctcattct gaggaatgaa aatggaccga gtctggaggc gctcattgtc
      241 gattattctg atgaaattgc atataatata catgatatgg aagatggact ggaaagcgga
      301 attctgagta ctgaaacact ttccgaaatt cctctctggg attatttctg gaaaaaagga
      361 tctgcatttg aaagagagaa cggatcggaa ctgcatacca gaaaaacttt gagaagcgtg
      421 atgaatgcaa tggtgactga tctgattcag aacatatcgg accagattca ggagcataaa
      481 attcagagca gagaaaattt ttcagaactc tggcgtaatg gaaaaagaat tataaattac
      541 tcagaaaaaa tgcagtcaga aattctgaat atgaaacgct ttctgaacga aaaactgtac
      601 cagaatcctg cagtaaaaaa caagtcagac tacggaaaaa aaataattga aaggctgttt
      661 tattttttcc agaaaaattt ttctgagatt cctgagtcct acagagaaag aaatactgaa
      721 agcagggaaa ggatcatctg tgattatatt gcaggaatga cagacagata tgcagaaaag
      781 aaaactactg aatacggact gaattgaaac ttctgattgt tgaatcaccc gcaaagataa
      841 agacttttca gaaagttctc ggttccgaat tcattgtttc cgcatgtttc gggcatgtgg
      901 cagaccttcc caaaggtgaa ttcgggatag acatcgaaaa agactttcag ccggaatgga
      961 aactgcttcc taaaaaatct gcattgataa aaaagctgaa agcggatgca ggcagatctg
     1021 aatctgtata tcttgcaact gaccctgaca gagagggcga ggcaattgca tggcacctgc
     1081 agaggcttct gggaaaaaga aaatatttca gagtaagatg cactgaaatt actgaatcag
     1141 gaatcagagg tgcagttcat aacctttctg aactcagaac agagactgta gattctcaga
     1201 tctcaagaag gcttgcagac agaattatcg gatacagaat cagtcctaga ctttgggata
     1261 tcagaagcgg actttctgca ggcagggttc agtcagtcgc tctgaaatgg atagtgcaca
     1321 gagaaagaga tatcagaaaa ttcattcctg aagactatta tgaaatcagt cttaagacag
     1381 aagtatccgg cattcctctt cttttcagat tcgttcagaa aaataatcct tcctcggcaa
     1441 aacttacaga agaagaaaag aacagaatcc tttcagaatt cagactgacc gaaattcaaa
     1501 gcggaaaaga tgcgcatcta atgcctcccg ctgaaataaa aatcatttca caaaatgaca
     1561 aaatcattaa aaaatatccc cctcctgcat ttacaacctc ctctctgcag aaggaggcat
     1621 atgccagatt caggatgcct gttgccaaaa caatgcgaat tgcacagaaa ctctatgagg
     1681 gcggaacgga aaaaggcggt ctcatcacct atatgagaac agattccact cgagtcagca
     1741 ctgagagaaa aaacagggc
//
LOCUS       DAOQGJ010000912         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48549_length_1759_cov_2.774061, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000912 DAOQGJ010000000
VERSION     DAOQGJ010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48549_length_1759_cov_2.774061"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1438)
                     /locus_tag="PK683_13510"
     CDS             complement(278..1438)
                     /locus_tag="PK683_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH09510.1"
                     /translation="MYQAFTEEQLQIRDLVRDFTRREITPVAHIWDEKNEHPTELINK
                     MRRELGINGLTIPEEFGGMGYGSVEQCLVTEEMSRGCLAIALGFGYTGLGMLPIMKGA
                     TDEQKKKWLPPVVAGEMGISFCLSEPGAGSDVPGMTTRAEKKGDKYVLNGTKQWITGA
                     GDAGAYTVFAYTDRGRGTRGVSCFFVPRNTPGLIVGKKEDKLGIRASGTHQVIFEDCA
                     VPAENLIGKENLGFIYALHTLNASRPYVAAMGVGVAQAALDYGARYAREREQFGQKIS
                     TFQAVQHMLADMSIIVETGREITYRAARMSDANDPNLAKYSAIAKAVTSEGAVKCALD
                     AIQIHGGYGYTKEYPVEKLLRDAKILCIFEGTTQIQKNEIAAYVIKEAATKK"
BASE COUNT          462 a          425 c          338 g          534 t
ORIGIN      
        1 cagcagtttc gaagtttgcg aaaccgccgg ttggcttatc caagaacccc cacctgcagc
       61 cgcaggcgta aggtgagtaa gtaaaaagca tagtatatag ccctttgctc aaaaaagcaa
      121 tggccttgaa tccgtatttt ttaaggaaca ttcgtgccga ctaaaaagca aatgtcttca
      181 gaaagactgt ggatctcgga gctgttaaag agcagtacat aaaaaaagca gcgaagaatt
      241 tcttcccgct gcttcttgtc cggttcgaaa acgcggatta ttttttagtt gcagcttcct
      301 tgattacata agcagcaatt tcgtttttct ggatctgagt agttccttca aaaatacaga
      361 ggatcttagc atctctcaga agtttttcca caggatattc ttttgtataa ccgtaaccgc
      421 cgtgaatctg aattgcatcc agagcacatt ttactgcacc ttctgaagta actgcctttg
      481 cgattgcaga atattttgca aggtttggat cgtttgcatc agacattctt gcagcacgat
      541 aagtaatttc gcggccggtt tctacgatga tagacatatc tgctagcata tgctgaactg
      601 cctggaatgt actgatcttc tgtccaaact gctctctttc tcttgcatat cttgcaccgt
      661 aatccagagc agcctgtgcc accccgacac ccattgccgc aacgtatgga cgggatgcat
      721 tcaatgtatg caatgcataa ataaatccaa ggttttcttt tccgatcagg ttttctgcag
      781 gaacagcaca gtcttcaaaa atgacctgat gagttccgga agcacggata ccgagcttgt
      841 cttctttttt tcctacaatc agtccaggcg tatttcttgg aacgaagaaa cagctgacac
      901 ctctcgttcc gcgtcccctg tcagtgtagg cgaaaactgt ataagcgcct gcatcacctg
      961 caccggtaat ccactgctta gttccgttca gaacatactt gtcgcctttc ttttctgctc
     1021 ttgtagtcat accaggaaca tcagaaccgg caccgggttc agaaagacag aaggatatcc
     1081 ccatctctcc tgcaacaact ggaggaagcc attttttctt ctgctcatcc gttgcacctt
     1141 tcataattgg aagcattccg agaccggtat aaccgaatcc gagagcgatt gcaagacagc
     1201 ccctgctcat ctcttcagtt actaggcact gctctactga accgtacccc atccctccaa
     1261 attcttcagg aatagtaaga ccgtttattc ccaattctct tctcatcttg ttgatgagtt
     1321 ctgtcggatg ttcatttttc tcatcccata tatgcgcaac tggagtgatc tcacggcgag
     1381 taaagtctct gaccagatcg cggatctgca attgttcttc agtaaatgcc tgatacatac
     1441 acatcatcct ctttaggatt tttcacccat ttggaagggt tcaatcccca tatttttttc
     1501 attgaatttt aaagcaaacg gttttcaatt tatctcattt actctctgtt caaaatctca
     1561 ttttgaataa agttttatcc tagacaaatt ttacagtggt aaatttttcc gtttttttca
     1621 ttcatttcag aaggggaaaa ccggcgggac tcataaactc agtctcagtc tctgattcag
     1681 catcaaagtc cggaaggcag caatttcttt ccaggatttt tcagcgtctg ttttcgataa
     1741 aatccgcaca tacgggaag
//
LOCUS       DAOQGJ010000913         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48574_length_1758_cov_4.462713, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000913 DAOQGJ010000000
VERSION     DAOQGJ010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48574_length_1758_cov_4.462713"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /gene="thrS"
                     /locus_tag="PK683_13515"
     CDS             complement(<1..1414)
                     /gene="thrS"
                     /locus_tag="PK683_13515"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNH09511.1"
                     /translation="MSVKVQLKDGSVREAEAGTKVSEFLKSLSISLAKSALAAKVDGK
                     EVDLFYPITKDCTLEALTFESKEGKEVFHHSSAHLLGQAVQRLYPKALLTIGPTVDNG
                     PGFFYYDIDFGDTVITQEDLPKIEKEMQKIVKENIEVRREDWKREDALKRFKESGEKY
                     KAELIESFQSDTVSLYYQGEWYDLCRGPHIPKTGLIKAFKLTAISGAYWKADSSNKML
                     QRIYGVSFPSQKELDEYIFMMEEAKKRDHRKLGAELELFTTSEKIGPGLVLWLPRGNV
                     IKEQLERWGKDTEEEWGYQRVTTPVITKETLFYTSEHLPHYKDHMFPAMEMDNENYYI
                     KPMNCPFHHTIFADRPRSYRELPLRIAEYGLCHRYEDSGTLFGLMRVRAMSMNDAHIY
                     CTEEQAVEEFKNVILLHEYYYKTLGIKDYFMVLALRNPSSAKYHGDDEMWSKAEALTR
                     RAMEESGVKFIVENDGAAFYG"
BASE COUNT          431 a          419 c          362 g          546 t
ORIGIN      
        1 gaccgtaaaa agctgctccg tcattttcaa caatgaactt cacaccggat tcttccattg
       61 cccgtcttgt cagtgcttct gccttgctcc acatctcgtc atcaccgtgg tatttcgcac
      121 tggaaggatt gcggagagca agcaccataa aatagtcttt gatgccgaga gtcttgtaat
      181 agtactcatg cagaagaatc acattcttga attcttccac cgcctgttct tctgtgcagt
      241 aaatatgagc atcattcata ctcattgcac gcactctcat cagaccgaac agagttcccg
      301 aatcctcata tctgtgacag agtccatatt ccgcaatgcg cagaggaagt tcacggtagc
      361 ttctgggtct gtctgcaaaa atggtatgat gaaaaggaca gttcatcggt ttgatgtagt
      421 aattttcatt atccatttcc attgcaggaa acatatggtc tttgtaatga ggaaggtgct
      481 cgcttgtata gaaaagtgtt tcttttgtaa tgactggggt tgtcactctc tggtatcccc
      541 attcttcttc cgtgtccttt ccccatcttt cgagctgttc tttgattacg tttcctctcg
      601 gaagccagag cacaagtccc ggaccgattt tttcagatgt ggtgaaaagt tcgagttccg
      661 ctcccagttt tctatggtcg cgttttttcg cctcttccat catgaaaata tattcgtcga
      721 gttccttttg ggaagggaag gatactccgt agattctctg aagcatttta ttggagctgt
      781 ctgccttcca gtaggcgccg gaaattgcag tcagcttaaa cgccttgatg agtccggttt
      841 ttgggatatg cggtcctcgg cagagatcat accattctcc ctgataatat aatgatactg
      901 tatccgactg aaaactttcg atcagctcag ccttgtattt ttcgccggat tccttgaaac
      961 gtttgagagc atcttctctt ttccagtctt cgcgccggac ttcaatattt tcctttacga
     1021 tcttctgcat ttccttttcg atcttaggaa gatcttcctg ggtaatgacc gtatcgccga
     1081 aatcaatatc gtaataaaaa aagccgggac cattgtccac agtcggccca attgtgagaa
     1141 gtgccttagg ataaagcctc tgaacagcct gaccgagaag gtgtgcactg gaatggtgga
     1201 acacttcctt tccttccttg ctttcaaatg tcagagcctc gagagtgcag tccttcgtga
     1261 tcggataaaa aagatccact tcttttccgt ccacctttgc ggccaacgct gacttggcta
     1321 aactgatgct taaactcttc agaaactcag acacttttgt gcctgcttct gcttctctga
     1381 ctgaaccgtc ttttaactga accttgactg acatatagcc agatttttca aaatgttcag
     1441 agtttcaatt cttaaatcag ctgggtctga ctgaaaactc tcttgggata aagtctgtcc
     1501 gagaacgttc ggaaaaatca gatcttaccg aattcaaaat catcctgact attttccggt
     1561 aaaaattgct tagaactttt ctgcaatttg ggaaacctaa cagtttaaca gcttgcggag
     1621 ctttagccag tttccagctc tgggcggcca ttgcgagagc agcagaagcc ctcaagccgc
     1681 cccacctgcg gaaggtgagt tactgaataa aagctgattt ttaaacgaca acgttcttga
     1741 tctgcatatc tttttcat
//
LOCUS       DAOQGJ010000914         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48598_length_1758_cov_2.554316, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000914 DAOQGJ010000000
VERSION     DAOQGJ010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48598_length_1758_cov_2.554316"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PK683_13520"
     CDS             complement(<1..364)
                     /locus_tag="PK683_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH09512.1"
                     /translation="MIEDSELRDLFQIESEEHLQIIEKGLLELEKNPNDLETLHLIFR
                     EAHSMKGASRMLGLSDIESISHLFEDMLGKASRGEFMITSDLIDTFYYALDATRKMVG
                     ESVSGELSGVDVVDVLDRL"
     gene            complement(366..>1758)
                     /locus_tag="PK683_13525"
     CDS             complement(366..>1758)
                     /locus_tag="PK683_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH09513.1"
                     /translation="FRTVAQANDGGNEVYYSDFENYPPSSNEPSSFLGMAIVVDGKTE
                     GVLIAQLPLDTVSSRLSRSFKEGGKEISYFVGSDFLARNDIEEKPKESWILKKKIDND
                     AVRKALSGENGVLIQDGILGSKAYIAYAPVLLHGVKYALVVETNYQETMEPLNHFEKQ
                     IMFVSLSVLAGVLLAVYLLSRSMSRPVVEAINVLSTSVREISSVIDNHESTANMQFAS
                     VNQTSTTIMNLGVSSRIAAEESGTVTAKAQEAQYASGIGHEMVMEMIQAINEMKTKVS
                     GIAEQIMRLSEKNNQISNIIGLVSELANETNMLALNAAVEAARAGENGKGFEVVAVEI
                     RKLADESKKSTLKIQEIINEIKRATDATVMVTEEGVKKAEESSKIGNKVLNSFNGITN
                     SVGIVFESIEKISLNIRQQSVSINEMVTAMNSINKGSQETATGIAQTKVGITQISDAT
                     HKLKLLIEGDKSV"
BASE COUNT          422 a          443 c          348 g          545 t
ORIGIN      
        1 gaagccggtc gagcacatcg acgacatcaa ctcctgacag ttctcctgat acagactctc
       61 ccaccatctt tcttgtggca tcgagtgcat aatagaatgt atcaatcaga tcggaggtga
      121 tcataaactc tcctctgctt gcctttccca gcatatcctc aaagagatgg gatatggatt
      181 ctatatcaga gagtccaagc attctggaag cacccttcat actgtgagcc tctctgaaaa
      241 tcagatgcag agtctccaga tcgttcggat tcttttccag ttccagcagg cctttttcaa
      301 tgatctgcag atgctcttcg ctctctatct ggaaaagatc tctgagttcc gaatcttcta
      361 tcatatcaga ctgatttgtc tccttcaatc agaagtttca gtttgtgtgt cgcatccgaa
      421 atctgagtaa tccccacttt cgtctgcgca attccagtcg ctgtctcctg agatcccttg
      481 ttgatggaat tcattgcagt gaccatttcg ttgatcgata cggactgctg tctgatattc
      541 aaggatattt tttctatact ttcaaataca attcctactg aatttgttat cccgttgaaa
      601 gaattcagaa ccttgtttcc tatcttggaa ctttcttccg ccttcttgac tccttcctcc
      661 gtcaccatta cagtggcatc tgtagctctc ttgatttcgt ttatgatttc ctgaatcttc
      721 agagtggact tcttgctttc atcggccagc tttctgatct cgactgcaac aacttcgaag
      781 ccttttccgt tttcgcctgc tcttgcagct tcaacggcag cattcagtgc aagcatattc
      841 gtttcatttg caagctcact cacaagcccg ataatattgc tgatctgatt gttcttttca
      901 gaaagtctca taatctgctc ggcaattccg gaaacctttg tcttcatttc attgattgcc
      961 tgaatcatct ccatgaccat ttcatggcct atgcctgatg catactgagc ttcctgagcc
     1021 ttggcagtca ctgtgcctga ctcttccgct gcgattctgg aagaaacacc cagattcatg
     1081 attgtggtgg aggtctggtt cactgaggcg aactgcatat ttgcagtgct ctcgtgattg
     1141 tctatcactg aactgatttc tctcactgag gtggacagaa cattgatggc ttccacgaca
     1201 ggcctgctca tggaacggga cagaagatag actgcaagca ggacacctgc cagcacggag
     1261 agagaaacaa acataatctg cttctcgaaa tgattcagag gctccattgt ctcctgatag
     1321 tttgtttcca caacaagagc atatttcact ccgtgaagca ggacaggagc gtatgcaata
     1381 tatgccttcg atccgagtat gccgtcctga atcagtacac cgttttcacc ggaaagagcc
     1441 tttcgaactg catcattgtc tatttttttc ttgagtatcc aggattcctt aggcttttcc
     1501 tcaatgtcat ttctagccag aaaatcagaa cctacaaaat aactgatctc ctttccgcct
     1561 tccttgaaac ttctgctgag tctggaactg actgtatcga gaggaagctg ggcaatcaga
     1621 actccttctg tctttccgtc cacgacaatt gccatcccga gaaagctgct cggttcattg
     1681 gaagaaggag gatagttttc aaagtcggaa taataaactt cattgccgcc gtcatttgcc
     1741 tgagcgactg ttctgaaa
//
LOCUS       DAOQGJ010000915         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48618_length_1757_cov_4.513514, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000915 DAOQGJ010000000
VERSION     DAOQGJ010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48618_length_1757_cov_4.513514"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PK683_13530"
     CDS             complement(<1..407)
                     /locus_tag="PK683_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09514.1"
                     /translation="MNFTVKIFLLTASFISVLFSFSACTPAEWKKLSAEKGVIDLRNR
                     EFNEDQYYALRGEWNFHYGKFIPCDKVGAEQKKEFLKIPGYWNGMQTDSGTLEGTGYA
                     TLHLKVLLPELKKNLAKSRKIIRFFHRKSWRTD"
     gene            complement(421..1551)
                     /gene="alr"
                     /locus_tag="PK683_13535"
     CDS             complement(421..1551)
                     /gene="alr"
                     /locus_tag="PK683_13535"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNH09515.1"
                     /translation="MKSQTWIEISKEAIRQNIQSFRNIIPAKTKLAAIVKSNAYGHGM
                     NLVAKEAVCSGADVLGVNSVQEALALSQLVPEIPILIMGSIHRLKGLNKVLNNERFWI
                     VVSRIDEVETLASFTPRPKIHLKTDTGMARLGYSGQYMLETLYNISSKQLPLDGIMTH
                     FASTEDFTEHSYTKKQLENFNDYIRFAFSLGYTNLVKHASASASTMLFEDTHLDMVRI
                     GISLYGLWPSIKTRLSLSLAGKDFILKPALEWKTRIQHIQNIPAGSFVGYGSTFRTNT
                     PSRIAVLPVGYYEGYNRRLSNQSYVLVRGERANVAGRVCMNMTMIDVTHIKECKEGDE
                     AVLIGNDGNESVTADFLADVSGTINYDIVTSLNKEIMRVLAH"
     gene            complement(1553..>1757)
                     /locus_tag="PK683_13540"
     CDS             complement(1553..>1757)
                     /locus_tag="PK683_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNH09516.1"
                     /translation="VLLPMKKGMELMKQIRGHISGIAVPQYVADLTGGGGKVPIPTDY
                     LLKEEVNGYYFRNYKGEVYKKGK"
BASE COUNT          464 a          400 c          344 g          549 t
ORIGIN      
        1 aaatcagttc tccacgactt cctgtgaaaa aatctgatta tttttctcga tttcgcaaga
       61 tttttcttca gttcagggag aagaactttc agatgaagag tcgcatatcc tgttccttcc
      121 agagtgcctg aatctgtctg catcccgttc cagtatcccg gtatttttaa aaattccttt
      181 ttctgttctg cgccgacttt gtcgcatggg ataaattttc cataatgaaa attccactct
      241 ccgcgaagcg cataatactg atcctcattg aactccctgt tccgcagatc aattacaccc
      301 ttttctgcac tgagtttttt ccattctgca ggagtgcatg ctgaaaagga aaaaaggaca
      361 gaaatgaatg aagcagtaag aagaaatatt ttaacagtaa aattcatgat aagttctcag
      421 tcagtgcgcc agaactctca taatttcttt gttgagcgaa gttactatat cgtaatttat
      481 cgttcctgaa acatcggcca gaaagtcggc agtcactgat tcattgccgt catttcctat
      541 cagtacagcc tcatctcctt ccttgcattc cttaatatga gtcacatcta tcattgtcat
      601 attcatgcag accctgcctg cgacattggc acgttcgcct ctgacaagca cataagactg
      661 attggaaagt cttctgttat agccttcata gtatcctaca ggaagaacag ctattctgga
      721 gggggtattt gttctgaatg tggaaccgta acctacaaag cttccagcag gaatattctg
      781 tatatgctga attcttgttt tccattccag tgcaggtttc agaatgaaat cctttcctgc
      841 cagacttaag gaaagccttg tcttgatgct cggccaaaga ccgtacagtg agattccgat
      901 cctgaccata tccagatgag tgtcttcgaa aagcattgtg gatgcagatg cagaagcatg
      961 tttgacaaga ttggtatagc ccaggctgaa tgcaaaacgg atgtaatcat tgaagttttc
     1021 cagctgtttc tttgtataag aatgttcggt gaaatcctca gtgcttgcaa aatgtgtcat
     1081 gatgccgtct agcgggagct gttttgaaga gatattatat aatgtttcca gcatgtactg
     1141 accggaatat cccagtcttg ccattccggt atcagtcttc agatgaattt taggtctggg
     1201 agtaaatgag gcaagtgttt ccacttcatc aatcctggac actacgatcc agaatctttc
     1261 attgttcaga accttgttca gacctttcag acgatgaatc gatcccataa tcaggattgg
     1321 aatttcaggg accagctgac tgagagccaa tgcttcctga acagaattga ctccgagcac
     1381 atctgcaccg gagcagactg cttccttggc cacaagattc attccatggc cgtaagcgtt
     1441 tgactttacg attgcagcga gtttcgtttt tgcaggaata atatttctga aactctgaat
     1501 attctgtctg attgcttcct tggatatttc aatccaggtc tgacttttca tctcatttcc
     1561 cctttttgta cacttcacct ttataatttc tgaaatagta gccgttaact tcctctttca
     1621 gaaggtaatc agtaggaatc ggaaccttgc ctccgccccc agtcagatct gccacatact
     1681 gagggactgc aattccgctg atatgtcccc gaatctgctt catcagttcc attccttttt
     1741 tcatcggcaa aagaacg
//
LOCUS       DAOQGJ010000916         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48764_length_1754_cov_4.021778, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000916 DAOQGJ010000000
VERSION     DAOQGJ010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48764_length_1754_cov_4.021778"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PK683_13545"
     CDS             complement(<1..684)
                     /locus_tag="PK683_13545"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HNH09517.1"
                     /translation="MISIFFILLLSVSELSSVPFDAVEDIPVPKGFQRAKVEKESFAE
                     YLRRQKLNRKDDTVYLYNGRMKKNQYAQYAVLLTDIGTKDLQQCADAVIRLRAEYLYE
                     RRRYPEIHFNFTNGETVHFENYAEGFRLSGFHKEMSVWKKSGKKDYSYPSFRKYLDLV
                     FTYAGTSSLLRELKKVNISSIQAGDVFIQSRQPFGHAVIVMDTAFNSEGQRIFLLAQS
                     YMPAQNIHIL"
     gene            complement(681..1130)
                     /locus_tag="PK683_13550"
     CDS             complement(681..1130)
                     /locus_tag="PK683_13550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HNH09518.1"
                     /translation="MISRKKIIQTAAMTAAMLSAGSQIFAKDHEHKSDSTEPESDGKY
                     TKAMQAAVKCRLSAEICIAHCISEMGKGNKDLEQCARTASDVKATCEAFITMASTGSG
                     FTKQMAELCIKICKACEKECKKHASHHSFCKDCMTDCRSCAKELAKV"
     gene            complement(1236..1442)
                     /locus_tag="PK683_13555"
     CDS             complement(1236..1442)
                     /locus_tag="PK683_13555"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNH09519.1"
                     /translation="MDRRNIGTGDRNLRIVNAFLLIILSIAGFFSDKTALAVTSVSVY
                     MLLTGIAGICPVYMMFGFRTGGKK"
BASE COUNT          489 a          351 c          341 g          573 t
ORIGIN      
        1 taatatatga atattctgag cgggcatata actctgagcc agaaggaata ttctctgtcc
       61 ttccgaattg aatgcagtat ccattacaat gacagcatgt ccgaaaggct gtcttgactg
      121 aatgaatacg tcacctgcct gtatactgct gatatttact tttttcaatt ctctgagcag
      181 tgaagatgtg cctgcataag taaaaactag atccaggtac tttcggaaac tgggatacga
      241 ataatctttt tttcctgatt ttttccatac ggacatttcc ttatgaaaac cggagagtct
      301 gaacccttct gcgtaattct caaaatgaac agtctctccg ttggtaaaat taaaatggat
      361 ttccggatat ctgcgtcttt catagaggta ttccgcacga aggcgtatga cagcatctgc
      421 gcactgctgg agatcttttg tgccgatatc tgtgagaaga acagcgtact gagcatactg
      481 attttttttc atcctgccgt tgtacagata aacagtgtca tcttttctgt tcagtttttg
      541 gcgcctcaag tattccgcaa aagattcttt ttcaaccttt gctctttgaa atcccttagg
      601 tacaggaatg tcttccactg catcgaaagg tactgaactc agttcagaga cggacaggag
      661 cagaatgaaa aaaatactga tcaaaccttg gcaagttcct ttgcacatga tctgcaatct
      721 gtcatacagt ccttacagaa ggagtgatgt gaagcatgtt ttttgcattc cttttcacag
      781 gccttgcaga ttttaatgca gagttcagcc atctgtttag taaagcctga tcctgtggat
      841 gccatcgtaa tgaacgcttc acaggtcgcc tttacatcag atgcagttct tgcacactgt
      901 tccagatcct tgttaccctt gcccatttcg gaaatgcagt gtgcaatgca gatttctgcg
      961 ctgagtctgc attttacagc tgcctgcata gccttggtat actttccatc tgattccggc
     1021 tcggtgctgt ccgatttatg ctcatgatct ttggcgaaaa tttgacttcc cgctgaaagc
     1081 attgctgcag tcatcgcagc tgtctggata attttttttc tggatatcat atcttattct
     1141 cctgaatttt aaaagataat agattggaaa acgaaaattt gtcgaataga attccctttt
     1201 ctccgaaaaa gagaaactca ggctgattga tcaatttatt tttttccacc tgttctgaat
     1261 ccaaacatca tataaactgg gcagattcct gcaattcctg tgaggagcat atatacagaa
     1321 acggatgtga ctgcaagtgc agtcttgtct gaaaagaaac cggcaattga gagaatgatc
     1381 agcaggaaag catttacaat tcttagattc ctgtcgcctg ttccgatgtt tctcctgtcc
     1441 ataagactct ccattgcaga aagcctgaga ctttcattta tgacaggatg acattgattc
     1501 aattctttat gtcaatgtaa aaaagctgtt ctctttctgt aaaagctatg acaataatca
     1561 tagaatccca aacagagatt cattgatgtt ttaaatactt attcttaaga gtttttgatt
     1621 ttgtccgaag ttttaagaat acagggtgag tgacaagaag gaatgactgc agatgaatta
     1681 atctgcagta ttccttgttt tatttgaatg aagtattata ctttataaga ttatacttct
     1741 gtatctgcca gagt
//
LOCUS       DAOQGJ010000917         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48933_length_1751_cov_2.583137, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000917 DAOQGJ010000000
VERSION     DAOQGJ010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48933_length_1751_cov_2.583137"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PK683_13560"
     CDS             complement(<1..1048)
                     /locus_tag="PK683_13560"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH09520.1"
                     /translation="MGLDMQQLIGEFSNEANEFLEKAETEIIELEKNPDPEKINAVFR
                     YIHTVKGNSGMFEFQNLSRLSHALESLLGKFREGSLTINKEYIDLMLEAVDRLKLMIR
                     ELDKESSFDISSMTEQLKSAAAAEQKKTVTENIAEELTITEEDVIRAFTEDFLKEAAR
                     EAEKNHQFASVAVLDLIEQRFQSLGELAEFLSQFGMNIKMRHLLADSIPDLETPSDFL
                     PFAFLILSEQDIKEFLKKYDLRPKFILSLYSPIQLIQSKDTNQKQEKQAAENSDSYLR
                     VRSRLLDDLINLVGETIITRNQLFQRSAFLNDPEGGNILSRMSQLITQLHEKIMHTRL
                     QELNSINQRIQRVVR"
     gene            complement(1371..>1751)
                     /locus_tag="PK683_13565"
     CDS             complement(1371..>1751)
                     /locus_tag="PK683_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09521.1"
                     /translation="KDIKELAGRSLDVAENANKLLDEIVPSILKTAGLVQEIAAASEE
                     QSSNLKNFNTAISQLNNVSQQNAASSEELAANSELLNKHAETLDQTIAFFRVEETEKR
                     ILKAEQSGSGQSKVRDAKENMIKM"
BASE COUNT          446 a          395 c          324 g          586 t
ORIGIN      
        1 ccctgacaac gcgctgaatt ctctgattga tcgaattcag ttcctgaagt ctcgtatgca
       61 ttattttttc atgaagctga gtgataagct gactcatcct ggaaaggatg tttccgccct
      121 ccggatcatt caggaatgca gatctttgga acagctgatt tcttgtaatg atcgtctctc
      181 ccacaagatt aatgagatcg tcgagcaggc ggcttctcac cctcagatag gagtctgagt
      241 tttcggccgc ctgtttctcc tgtttctggt ttgtatcctt tgactggatc agctgtatcg
      301 gactgtaaag agagagaatg aattttggac gaagatcata tttctttaaa aactctttaa
      361 tgtcctgctc ggacaagatc aaaaaagcaa acggaagaaa gtcagaggga gtttccaaat
      421 ccggtatgct gtctgcaaga agatgtctca ttttaatgtt cattccgaac tgtgaaagaa
      481 attcagccag ttcacctagg gactggaatc tctgttcgat cagatcgaga actgcgacag
      541 atgcaaactg gtgattcttt tctgcttccc ttgcagcctc tttcaaaaaa tcttcagtga
      601 atgctctgat aacatcttct tctgttattg taagttcctc ggcaatattc tcagtcaccg
      661 tctttttctg ttcagccgca gccgcgcttt tcagctgttc tgtcatagaa ctgatgtcaa
      721 aactgctctc tttgtcaagt tcacggatca tcagcttcag ccgatctaca gcttccagca
      781 tcagatcaat gtactctttg tttattgtca gagaaccttc cctgaatttg cccagaagac
      841 tttccagtgc atgtgaaagc ctgcttaaat tctggaattc gaacattcca gaatttcctt
      901 ttactgtatg tatatatctg aaaactgcat tgatcttttc cggatctgga tttttttcca
      961 gttcgataat ctctgtttca gccttttcca aaaattcatt agcttcattg gagaattctc
     1021 caatcagctg ctgcatatcg agtcccatat cagcctctta ctgaagacag aaaatctgtc
     1081 atattaagtt cattaaaagc tgcctcaagc agtttttctg attcaataaa aacagatata
     1141 ttccttttct tcaaactttt tataaaagag cagagcagaa agaagtgctg tattggaaga
     1201 tcctcgattt ctccgaactt taagaaaaat ttcaaatccg atgcaacctg aaaaatttcc
     1261 aagagacttt ttctgaaatc ctcaggagaa tactgcaaat ctctttcctc tagaaaaact
     1321 gtaacagcct gcttttcagg cttttttatc aggttcatct tttttttccg tcacatcttt
     1381 atcatatttt cttttgcatc ccgaacctta gactggcctg aacccgactg ttcagccttt
     1441 aagattctct tttctgtttc ctccactctg aagaatgcaa tcgtctgatc aagcgtttct
     1501 gcatgtttgt tcagaagttc cgagtttgcc gccagttctt cggaagaagc tgcattctgc
     1561 tgagatacat tgttcagctg gctgatagcc gtgttgaaat ttttcagatt ggagctctgt
     1621 tcctctgaag cagctgcaat ttcctgaaca agtccggccg ttttcagtat gcttggcaca
     1681 atttcatcca gaagtttatt tgcattttct gctacatcca gactgcgtcc ggccaattct
     1741 ttgatgtctt t
//
LOCUS       DAOQGJ010000918         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48951_length_1750_cov_3.510914, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000918 DAOQGJ010000000
VERSION     DAOQGJ010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48951_length_1750_cov_3.510914"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PK683_13570"
     CDS             <1..251
                     /locus_tag="PK683_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNH09522.1"
                     /translation="CILITKLEGSSGCSALSGDISIGVQGFLVENGERVHPVDGITMN
                     TNYFDLLYKISAASDEYSDSYSSIKVPDLLIESVNIAG"
     gene            291..1151
                     /locus_tag="PK683_13575"
     CDS             291..1151
                     /locus_tag="PK683_13575"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNH09523.1"
                     /translation="MIKVLLFCFFTVFPFLLSADDLKIPALTGRIVDETFTLTEADKS
                     ALTDELKKLETEKGSQIAVLVIDTVGELGIEEYGIKVAEAWKLGRKDVSDGAILIVAL
                     KDKKMRIEVGRGLEGFLTDLRTKQIQEKIIKPEFKNGNFSGGIRQGTEAMITLARGAE
                     LPLPEETPEKLEGTTLATIVFCFILLGLFLEIRFYNLIWRILIAVLSVFFIYWYVNMF
                     SSFTAAVISSSVSAFFFMLPIVFRSSWGGGDGYSGGGFFSSDSSSGSSGGFFGGGGSF
                     GGGGSSSSWD"
     gene            1178..>1750
                     /locus_tag="PK683_13580"
     CDS             1178..>1750
                     /locus_tag="PK683_13580"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNH09524.1"
                     /translation="MNLLLKIIIFLFPFFLSAEDEPKIPELKSRVMDEASVLTESDKT
                     AIINELKELEKSKGSQIAVLTVDTTGELTISEYGIKVAEKWKIGRKGVSDGAILIVAV
                     KDKRMRIEVGRGLEEFLTDLRTKQIQERIIKPEFKKKNMSGGIRLGVEAMVTVAKGAE
                     LSLLDLPKKEDKGWNNSSQPKQFLFFAFFVG"
BASE COUNT          499 a          326 c          446 g          479 t
ORIGIN      
        1 aatgcattct gattacaaaa ctggagggaa gttccggatg ttcggctctg tcgggagata
       61 tttcgattgg tgtgcaggga tttcttgtgg aaaacggtga gagggtgcat ccggtggacg
      121 gaatcacgat gaatacgaat tattttgatc ttctttataa aatttccgca gcctcagacg
      181 aatattctga ttcctattcg tccataaaag tgcctgatct tctgattgaa tcagtgaata
      241 ttgcggggta gaaactgagt atgagcagta taatgaaaaa acatttttat atgataaaag
      301 tcctcctttt ctgtttcttc acagtatttc cttttcttct gagtgctgat gatctgaaaa
      361 tccctgcttt gacaggcagg attgttgatg agactttcac tctgacagaa gctgacaagt
      421 ctgcactcac ggatgaactg aaaaaactcg aaacagaaaa gggttctcag atagcagtgc
      481 tggtaatcga tactgtaggt gaacttggca tagaagaata cggaatcaaa gtcgctgaag
      541 cctggaagct cggccggaag gatgtgagtg acggagccat tctgatcgtt gcattgaaag
      601 ataaaaagat gagaatagag gtcggccggg gtctggaagg atttctgact gatcttcgca
      661 ccaagcagat tcaggaaaag atcattaagc cggaattcaa aaacggaaat ttttcagggg
      721 gaatcagaca gggtactgaa gcaatgatca ctcttgcaag aggcgctgaa cttcctctgc
      781 cggaagagac tcctgaaaaa ctggaaggta cgaccctggc aacaattgtt ttctgtttta
      841 ttctgctggg actttttctg gaaatcagat tttacaatct gatctggcgg attctaattg
      901 ccgtactgtc tgtatttttc atttactggt atgtaaatat gttcagcagt tttactgctg
      961 cggttatctc ctcctcagtc agcgcgtttt tctttatgct gccgatcgta tttcgttcga
     1021 gctggggcgg cggtgacggt tattccggag gaggtttttt ctcctctgat tccagttcag
     1081 gaagttcggg cggatttttc ggaggcggcg gatcatttgg gggaggcggc tcatcaagca
     1141 gctgggatta aaaactatga aaaaaggcag ttcaatcatg aatttattac tgaaaatcat
     1201 tatatttctt tttcctttct ttctgagtgc agaagatgaa ccgaaaatcc ctgaactgaa
     1261 gagcagagtg atggatgagg cctctgttct gactgaaagc gacaagacag ccatcatcaa
     1321 tgagctgaag gaactcgaga agtccaaagg ttctcagatt gccgttctga cagtcgatac
     1381 gacaggtgaa ctcacgatat ccgaatatgg aatcaaggtc gcagagaaat ggaagatcgg
     1441 aagaaaagga gtcagcgacg gagcaatact gattgttgca gtcaaggaca aaagaatgag
     1501 aattgaggta ggtcgcggac tcgaagaatt tctgactgat cttcgaacaa agcagattca
     1561 ggaaagaatc atcaagcccg agttcaagaa gaagaatatg tccggcggaa tccggctcgg
     1621 agtggaagcg atggtgacag ttgcaaaagg tgcagaactc tctcttctgg atctgcctaa
     1681 gaaagaagac aagggctgga acaattcttc acagccgaaa cagtttcttt tctttgcatt
     1741 ctttgtcggc
//
LOCUS       DAOQGJ010000919         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_48967_length_1750_cov_2.662537, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000919 DAOQGJ010000000
VERSION     DAOQGJ010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48967_length_1750_cov_2.662537"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PK683_13585"
     CDS             <1..411
                     /locus_tag="PK683_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNH09525.1"
                     /translation="VNIQAVSYKHRPERKVSIFRRSGKIGAGLGATTGWALLQELKAA
                     GAEMYTSLTYKEVNDIGLVVELKSGEVKTVECDSILICAGQERENSLAEEYMKRHPEK
                     QVFIIGGAKEASGLDAKRAILEGTMAARKIGIRD"
     gene            408..1235
                     /locus_tag="PK683_13590"
     CDS             408..1235
                     /locus_tag="PK683_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNH09526.1"
                     /translation="MNQFRTWKALVEEFSGSEYLETDPIKFPHRYRSREDIEVSAFIS
                     ALFSYGNVHSIFKFLEDLFLIFGNSPSAFLLHHNFKKSSVSFPYYRFQSPSDVQRFLR
                     IFQSLMQKEKTLETFFNGIKIHDGIINLQKGLISEHLKIYKENASRGLLFLIGSGSLK
                     SANKRYLMFLRWMSRRHFPDFGLYETVSPSHLLYPLDTHILKLSKILGMNQIQTADWK
                     SAELLTEKFREIFPKDPVQADFALSRLGILRLCRSRYEGSVCEKCSMRNLCTIYEAR"
     gene            1553..>1750
                     /locus_tag="PK683_13595"
     CDS             1553..>1750
                     /locus_tag="PK683_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNH09527.1"
                     /translation="MDNSAVPKDFLTGLKENWKNDIISGFTVFLIALPLCIGISLASG
                     APPLAGIFSAIVGGVVISLLSG"
BASE COUNT          521 a          314 c          383 g          532 t
ORIGIN      
        1 gtaaatatcc aggctgtgag ttataagcac cggcctgaaa gaaaagtttc cattttcaga
       61 agatcaggaa aaataggagc ggggcttgga gcaactaccg gatgggcgct tcttcaggaa
      121 ctgaaagcag caggtgcgga aatgtataca tcgcttacct ataaggaagt caatgatatc
      181 ggccttgttg tggaacttaa gtcgggagaa gtcaagactg ttgaatgtga ttctattctg
      241 atctgtgccg gtcaggaaag agagaattcc cttgctgagg aatatatgaa gagacatcct
      301 gaaaagcagg tctttatcat aggcggtgcc aaggaagctt caggactgga tgcaaaaagg
      361 gcaatactgg agggtacaat ggcagcaagg aaaatcggaa tacgggattg aatcagttca
      421 gaacctggaa agctttggtg gaagagtttt caggttctga gtatctggaa acagatccga
      481 tcaaatttcc tcacagatac aggagcagag aagatatcga agtctctgct tttatatctg
      541 ccttattctc ctacggaaat gtacattcca tttttaagtt tctcgaagat ctgtttctta
      601 tattcggaaa cagtccttct gcttttcttc ttcatcataa ttttaaaaaa tcttcagtca
      661 gctttcccta ctacagattt cagtctccat ctgatgttca aagatttctg agaatctttc
      721 agtctctgat gcagaaggaa aagactcttg agactttctt caatggcata aaaattcatg
      781 atggaatcat taatcttcag aaaggactga tttctgaaca tttgaaaatt tacaaagaga
      841 atgcaagtcg cggacttctg ttcctgattg gatcaggatc attaaagtct gcaaataaaa
      901 gataccttat gtttttaaga tggatgagca ggagacattt tcctgacttc ggtctctatg
      961 aaactgtgtc tccgtctcat cttctctatc ctctggatac acatatactg aaactctcaa
     1021 agattctggg tatgaatcag atacagacgg cagactggaa atctgctgaa cttcttacgg
     1081 aaaaattcag agaaatcttc ccgaaggatc ctgttcaggc agactttgct ctaagcagac
     1141 tcggaattct gagactgtgc agatccagat atgaaggatc ggtatgcgaa aaatgcagta
     1201 tgagaaacct gtgcacaata tatgaagccc gttaataaaa tcatgtttca tctgcaattg
     1261 gatacatgtt ttaattcttc ctcaaaaaag gtatgacgtt aaagtatggt taaataaaat
     1321 tgcatttgac gttttaaatg agaggttgaa tgaaagattt ttatcatcag tgcagtgcaa
     1381 atattcataa cgatatttac ggttgctgct ttcactgtct tttgatgaat gtcttcattt
     1441 tgggaatcac atgaggggaa ccattgtttt tctgctcggt gaaacattgt gcatcagaca
     1501 atcggtgccg atataaattg caccgtctta aggtgcggaa aaaggattta gtatggacaa
     1561 ttctgcagta cccaaggatt ttttaactgg tctcaaagaa aactggaaaa atgatattat
     1621 atcaggtttt accgttttcc tgattgctct gcctctatgt atcggaattt cacttgcttc
     1681 aggagcgcct cctcttgcgg gaattttttc tgctattgtc ggtggggttg taatttctct
     1741 tctgagcgga
//
LOCUS       DAOQGJ010000920         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49003_length_1749_cov_3.131051, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000920 DAOQGJ010000000
VERSION     DAOQGJ010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49003_length_1749_cov_3.131051"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PK683_13600"
     CDS             complement(<1..806)
                     /locus_tag="PK683_13600"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNH09528.1"
                     /translation="MRLLRASLYLCIIFASAEMHSEDTPPDLLSFEGPSMHLAEYITV
                     LEDREGLLTVNEIIKPENSYRFRKVESSKEAFNFSYSSSAYWLRLPLKNSGNRVRDCT
                     LVISYPRLENIQLFFPDPKTGRYTVTHSGYSVPFQKRPYQSRFFVFPISVPEQFSDTL
                     YLRIESANSLSIPIMLWDKNSYYRHELDDHVIQAMYFGTVAAMVLFNILLYFILRDIN
                     YIIYVLFVLSSGLTVAAYNGIASEILWKNSPFLDKYSVSILFSSSLVIFLL"
     gene            complement(803..>1749)
                     /locus_tag="PK683_13605"
     CDS             complement(803..>1749)
                     /locus_tag="PK683_13605"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH09529.1"
                     /translation="FGSALEMILLAFALADRYNILRKEKEKAQEEVFEAQAKALQAEQ
                     QLVENLKASERLLEQKVQERTSELNSTLKIIRQDLSIAKKIQKNTLLINPAFQKELNI
                     AVSYIPMAEVGGDFYDIFKVNDCTYRIFQADATGHGVQAAMITMAIKGIYDNVKKFDL
                     DPSEIMEIFNNEYMIKYESLNSLMTAFIADIDTQKGTVKYVSAGHPAAMIQKDGGDLF
                     LNSTGRMIGIMKNVQYQSGKVQFSAKDRLFLFTDGIFEEFNERREMFGEERLYSVLSS
                     CRKLPIEEVFQTVLENLTQYMNGTKLQDDIAILGIEYR"
BASE COUNT          470 a          372 c          383 g          524 t
ORIGIN      
        1 agcaggaaaa tgacgagtga ggatgagaag aggatgctga cgctgtactt gtcgagaaag
       61 ggcgaattct tccagagaat ttcggatgct atcccgttgt atgcggcaac ggtcagacct
      121 gaagacagta caaaaagtac ataaattata taatttatgt ctctgagtat aaaatacaga
      181 agaatattga aaagcaccat cgcggccact gtgccgaaat acatagcctg gattacatga
      241 tcgtcaagtt cgtgtctgta ataggagttt ttgtcccaaa gcataatcgg tatgctgagt
      301 gaatttgctg actctattct gagatacaga gtatccgaaa actgttcagg aacggaaatc
      361 ggaaagacaa agaacctgct ctgataagga cgcttctgaa aagggacaga gtagcctgaa
      421 tgagtgaccg tatatcttcc agttttcgga tcaggaaaaa agagctgaat gttttccagt
      481 ctgggataac tgattaccag agtgcagtct ctgactctgt tccctgaatt tttcagaggg
      541 aggcggagcc agtatgcaga agaagaatag gagaaattga aagcttcctt tgaggattca
      601 acctttctga atctgtatga attttccggt tttattattt cattgactgt cagcaaaccc
      661 tctctgtctt ccagtacagt aatgtattca gcaagatgca ttgaagggcc ttcaaaggat
      721 agaaggtcag gcggagtgtc ttccgaatgc atttctgccg atgcgaaaat aatgcacaga
      781 tacagagatg ctctcagaag tctcatctgt attcaattcc cagaatggca atatcatcct
      841 gaagttttgt gccgttcata tactgagtca gattttcaag aactgtctga aaaacttcct
      901 caatcggaag ttttctgcag gaagacagga cggaatacag tctttcttcg ccgaacattt
      961 ctcttctttc gttgaactcc tcaaatatgc cgtctgtaaa cagaaaaagc ctgtcctttg
     1021 cggagaactg aactttgccg gactgatact ggacattttt cataatcccg atcatccttc
     1081 cggtgctgtt cagaaacagg tcgccgccgt ctttctggat cattgcagca ggatgtcccg
     1141 ccgatacata tttcaccgtg cctttctgag tgtcaatgtc tgcaatgaag gctgtcatga
     1201 gactgttcag tgactcatat tttatcatat attcattatt aaatatttcc atgatctcag
     1261 aaggatccag gtcgaatttt tttacattgt cgtagattcc ctttatggcc attgtgatca
     1321 ttgccgcctg aaccccgtgt ccggtcgcat cagcctgaaa tattctgtaa gtgcagtcat
     1381 ttactttgaa aatatcataa aagtctccgc ctacttccgc catcggaata tatgaaactg
     1441 caatgttgag ttctttctga aatgccggat tgatcagcag agtgtttttc tgaatctttt
     1501 tggctatgct gagatcctgc cggatgattt tcagggtgct gttcagttct gaagttcttt
     1561 cctgcacctt ctgttccagc agtctttcag aagccttcag gttttccacc agctgctgtt
     1621 cggcctgaag agcctttgcc tgggcctcaa aaacctcttc ctgggccttt tctttttcct
     1681 ttctgagaat attgtatctg tctgccagtg caaatgccag cagaatcatt tcgagggctg
     1741 aaccgaact
//
LOCUS       DAOQGJ010000921         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49022_length_1749_cov_2.347698, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000921 DAOQGJ010000000
VERSION     DAOQGJ010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49022_length_1749_cov_2.347698"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..788
                     /locus_tag="PK683_13610"
     CDS             90..788
                     /locus_tag="PK683_13610"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNH09530.1"
                     /translation="MQNIDYSKKIKKEERRLTPLHPVQETVSQKLKSANKPSALMQLI
                     QNRTQSIPPLYLFVGAALFFTSGLIIGMRIDHQESSFSAGQPEINSFKNVNNSAPQTN
                     SSAPSETESSSGDSDFNAETQAQPQKEEVKSAAKNQNIAVPKNIQFPPKENQVNYIIQ
                     LGSFNKEEAVHYAASLIKERQEFQGRIFRGTSTGKLFLGYFYDENEAKNALKRVKKFR
                     NGIFAEASIKNIQF"
     gene            791..1297
                     /locus_tag="PK683_13615"
     CDS             791..1297
                     /locus_tag="PK683_13615"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH09531.1"
                     /translation="MNSAAVSFEIKRYESGIQLLKDSIKDVLPEQMTLHPIPEKWSIA
                     ECIFHISDMESVFADRMKRIIAMDNPALISADENRYASELAYNKRDVKEEILLFTLTR
                     NQMLRILNSIPESSFVRTGVHSEAGTLSLFQVLKKSCTHLEHHLWHMNEKRMNYGLSL
                     LPIQPSSD"
     gene            1294..>1749
                     /locus_tag="PK683_13620"
     CDS             1294..>1749
                     /locus_tag="PK683_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH09532.1"
                     /translation="MKKKAAKKHSTKDQIRSSETRELLYQTAVALFRTEGYDETTMRK
                     ISAESGLSLGLLYYHFQSKQEIVLEFYKRTQQDSDRKCRNFFSQNKDLKKRILFIVKD
                     KLDQFKPYRNFLYVLSRSAGDPFDPLSPFSTSTAATRNEALEIISAALTD"
BASE COUNT          592 a          369 c          356 g          432 t
ORIGIN      
        1 ggagaattga cgatatagta ttttagggcg gaagcaaatg agtctgtcct gcctgtgaaa
       61 ggcacaaaaa aatacagggc ttgttgagaa tgcagaatat tgactactcc aaaaagataa
      121 aaaaagaaga aagaaggctg acgcccctac atcccgttca ggaaacagtt tctcaaaaac
      181 tgaaatccgc aaacaaaccg tcggcactga tgcagctcat acagaacaga actcagtcaa
      241 ttcccccgct ctatttattc gtgggagctg cgcttttctt tacctcagga ctcataatcg
      301 gaatgaggat agatcaccag gagagcagtt tctccgcagg acagccggaa ataaacagtt
      361 tcaaaaatgt aaataattct gcacctcaaa ctaacagctc tgccccgtct gaaacagaat
      421 cttcttccgg agactcagac tttaatgccg aaactcaggc acagccgcag aaggaagaag
      481 taaaatcggc agcgaaaaac cagaatatag ccgtacctaa aaatattcag tttcctccaa
      541 aggaaaatca ggtaaactac atcattcaat taggttcctt caataaggaa gaagcagtcc
      601 attacgccgc ttcgctgatc aaggaaagac aggaatttca gggaagaatt ttcagaggga
      661 caagtaccgg aaaactcttt ttaggttatt tttatgatga aaatgaagct aagaatgccc
      721 tgaaaagagt aaagaagttc agaaacggta tctttgctga agcctccata aaaaacatcc
      781 agttctgatt atgaattctg cagcagtaag cttcgaaatc aaaagatatg aaagcggaat
      841 ccagcttctc aaggattcca taaaggatgt tcttcctgaa cagatgaccc tgcatcccat
      901 tcctgaaaaa tggagcattg ctgagtgtat atttcatatt tctgatatgg aatcagtttt
      961 cgcagacagg atgaaaagaa tcattgctat ggacaatcct gccctgattt cggcagatga
     1021 aaacaggtac gcctccgaac tggcatataa taaaagagat gtaaaagaag aaatcctgct
     1081 ctttactctc accaggaatc agatgctcag aatactgaat tccattcctg agtcgtcatt
     1141 tgtgagaacc ggtgttcaca gtgaagccgg aactctcagt cttttccagg ttttaaagaa
     1201 atcatgtact catcttgagc atcatctctg gcatatgaat gagaaaagga tgaattacgg
     1261 actttctctc cttcctatac agcccagctc cgattgaaaa aaaaggccgc aaaaaagcat
     1321 tccacgaaag atcagatcag atcttcggaa acgagagaac ttctttacca gacggctgta
     1381 gctcttttca gaacagaagg ctacgatgaa acaacaatgc ggaaaatttc agctgaatca
     1441 ggtctttcct taggactgct ctactatcat tttcaatcga agcaggaaat agtgctggaa
     1501 ttttacaaga ggacacagca ggattcggat agaaaatgca gaaatttttt ttctcagaat
     1561 aaagatctga aaaaaagaat tctgttcata gtcaaggaca aacttgacca gtttaaacct
     1621 tacaggaact ttttatatgt tctgtccaga agtgccggcg atccgtttga tcctctttcc
     1681 ccgttcagta caagcacggc ggcaaccaga aacgaggctc tggaaatcat ttcggcagcc
     1741 ttgacagat
//
LOCUS       DAOQGJ010000922         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49090_length_1747_cov_3.902482, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000922 DAOQGJ010000000
VERSION     DAOQGJ010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49090_length_1747_cov_3.902482"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PK683_13625"
     CDS             <1..1121
                     /locus_tag="PK683_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNH09533.1"
                     /translation="LGEKYVAHAISMIMETFKMPIPGDMSVHGIAITLSFVLITFFHI
                     TLGELIPKSIAIQSAEAISLFASVPMMFFYYIFYPITFFMNGLTNAALRMLKIENTGH
                     KMVHSAEELMILLEEHTKQGKIDNEELKIIQNTFEFSEHEAKEVMTHRLSMVAIPETD
                     KIKDILPMIAEHRFSRYPVFKNTPDTITGVIHVQAVIDWLSHSSRNKNDAVMTIMQPP
                     VFVPETLSIEKVLQKLRHAKQHMAIVVDEYGGVSGLLTLEDIIEEVFGQIKDETDEED
                     PITVHDIRTDSYIIDGEAELDELSDVLEGENIEQNKDVRTIAGFFLEKHEDMPKEGSM
                     ITIRNGTLTVVKMDKNKIIHVRYDHKPVEIIPIKEDYD"
     gene            1360..>1747
                     /locus_tag="PK683_13630"
     CDS             1360..>1747
                     /locus_tag="PK683_13630"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HNH09534.1"
                     /translation="MENQAAAQAQTSVEEVKNLEQLSPSEQQRVDEIAKSINVTDSQG
                     VIQYGVGTQSKISEFSDSVLKEIKTKDAGENGEILTDLMLKIKDLDVESLSDSDGFLS
                     KIPLISSLVSSSKKFLAKYDTISVQIE"
BASE COUNT          581 a          321 c          398 g          447 t
ORIGIN      
        1 ggctgggtga aaagtatgtt gctcatgcaa tttcaatgat tatggaaaca ttcaaaatgc
       61 ctattccagg agatatgagt gttcatggaa tcgcaataac actgtcattc gtgctgatca
      121 cattttttca tattacactc ggtgagctga ttccaaaatc aattgcaatc caaagtgcgg
      181 aagcaatatc actgtttgca tcagttccaa tgatgttctt ctactatatt ttctacccaa
      241 tcactttttt tatgaacggc ctgacaaatg ctgctctcag aatgctgaaa atagaaaaca
      301 caggtcataa gatggttcac agtgcggaag agctcatgat tcttctggaa gaacatacaa
      361 aacagggcaa gattgacaac gaagaactca agatcataca gaatactttt gaattctcgg
      421 aacatgaagc aaaggaggta atgacccaca ggctgagtat ggttgccatt ccggaaacag
      481 acaagatcaa ggatattctg ccgatgatcg cagaacacag attttccaga tatcctgttt
      541 tcaagaatac gccagataca atcactggag tgattcacgt tcaggcagtt attgactggc
      601 tttctcattc ctccagaaac aagaatgatg cagtgatgac gattatgcag ccccctgtat
      661 ttgtaccgga aacactttcc atcgaaaagg ttcttcagaa gctcagacat gcaaagcagc
      721 atatggcaat tgtagtggat gagtacggag gagtttcagg acttttgact ctggaggata
      781 tcatagaaga agtgttcggt cagatcaagg acgaaacgga tgaagaagat ccgatcactg
      841 ttcatgatat ccgcacggac tcttatatca ttgacggaga agcggaactg gatgaacttt
      901 ccgatgtgct ggaaggagag aatattgaac agaacaagga cgtcagaaca attgcaggat
      961 tctttctgga aaagcatgag gatatgccga aagaaggaag tatgattaca atccggaacg
     1021 gtacactgac agtcgtaaaa atggataaga ataagattat ccatgtaagg tacgatcata
     1081 aacctgtgga aattattccg atcaaagagg attacgatta gaaaagagag tccgctctct
     1141 gtttatatta tgtgatcaaa agcaggcata gaatttgatc taagtaaaaa gatttgcagg
     1201 cagggtttta gcgcggcagg atgaaaccca cataccaagc acaggaaggg gctaatcctc
     1261 ctgtgataag gacaactttt ttcctgcttt tttaaaagca ggaattgaat aaatatcaac
     1321 tgtgaagctt gcgggcttgc agttacaagg aaggaaaata tggaaaatca ggcagcagcg
     1381 caggcacaga cttctgtaga agaagtaaaa aatctggagc agttaagtcc ttctgaacag
     1441 cagagagtcg atgaaattgc aaagagcata aacgtaactg actcacaggg agtcattcag
     1501 tacggagtgg gaactcagtc gaaaatctct gaattttcag attcagttct gaaagagatc
     1561 aagaccaagg atgccggaga gaacggtgaa atcctgactg atctgatgct gaaaatcaag
     1621 gatctggatg tggaaagtct ttccgactct gacggatttc tttccaagat tcctctgatt
     1681 tcctctcttg taagttcttc aaaaaagttt ctcgcaaagt atgatacaat cagtgtgcag
     1741 atagaaa
//
LOCUS       DAOQGJ010000923         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49105_length_1747_cov_3.063830, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000923 DAOQGJ010000000
VERSION     DAOQGJ010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49105_length_1747_cov_3.063830"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK683_13635"
     CDS             complement(<1..546)
                     /locus_tag="PK683_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09535.1"
                     /translation="MKFLFLLFLFPVYQVFSVPTTLRIEEKENYQFIESVRAKKFADR
                     EIDRLHQSIIKMLARMKEHETSGSLKNSAKYTANVPEEPEIVFKKDAEGKEYFSLFLS
                     QAVSPEDYPVYHIINTRVYVYPSEDLKTLKKVILQFNRVNANLENPNFPVYVKEMRRI
                     INNTPNFPEKQKTEGDLSAGIP"
     gene            672..>1747
                     /locus_tag="PK683_13640"
     CDS             672..>1747
                     /locus_tag="PK683_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09536.1"
                     /translation="MRGIKLTAAGLATLLVFVLNCEDKPKKAKVLTTTMSVLVDDPKA
                     AASTSTSTDGTSAGTGTTASGTAVTASVSAGNYSVSMPSGITSTASPSGDFTGITYAG
                     NPAEVAGFIGQVPDQGQADSASLSSSLLGIINAAIPTTNLATANVLSSSTSSGSGGST
                     SIYHLAVTTDSATNVTGLSNLLLQTIGTSITNGTVTNLPTSGGTESTSASFHVVLQVT
                     YSSTGPEVIGVGVSTTANYSANEALLTTFLDGSNIASGTVTKIAKTDSFSGAADPKAD
                     FVWVVDNSSSMQQEQSAVSSAATTFFSALSNKRLDYRIGVITTDSSTLRTAGGTSWVV
                     NSTSGGQTAFVANTAAGTGGSGNES"
BASE COUNT          483 a          417 c          380 g          467 t
ORIGIN      
        1 aggaatccct gcacttaagt caccttccgt cttctgcttt tccggaaaat taggggtatt
       61 gttgatgatt cttctcattt ctttcacata cacaggaaaa ttaggatttt ccagattggc
      121 attcactctg ttgaactgca gaatcacttt tttcagagtt tttaaatctt cggaaggata
      181 cacatagacc cgggtattaa taatatggta gaccggataa tcttccggtg agactgcctg
      241 actcaggaag agactgaagt attcctttcc ttccgcatct tttttaaata caatttccgg
      301 ttcttccgga acattggcag tgtacttcgc tgaatttttc aggcttcctg aagtttcgtg
      361 ctctttcatt ctggcgagca tttttatgat ggactgatgc agtctgtcaa tttctctgtc
      421 tgcaaatttt ttggcacgga cagactcgat aaactggtag ttttcctttt cttcaattct
      481 gagagtggta ggaacagaaa aaacctgata aacagggaac agaaagagca gaaacaaaaa
      541 tttcatgatt tacctcaaat atattatcga attccttttt ctcaataatg acttattttt
      601 acttgcattc agagtatcgg agtgtaacat ttcggaagga attcccgttt aataaagtaa
      661 ggagaaaatt tatgagagga atcaaactga cagcagccgg tcttgcgact ttgctcgtct
      721 ttgttctaaa ctgtgaagac aaacccaaaa aagccaaggt tctgacgact acaatgtcag
      781 ttctggtgga tgatcctaag gcagcagcct ctacgagcac ctctactgac ggtacatcag
      841 ccggaactgg cactactgct tccggaactg cagtgacagc ttctgtatct gccggaaact
      901 actccgtgtc catgccttcc ggtattacct ccacagcatc tccgtctggt gactttacag
      961 gaatcactta cgccggcaat ccggcagaag tggcagggtt tataggacag gtcccggatc
     1021 agggacaggc cgattcagca tcgctttctt ccagtcttct gggtatcatc aatgccgcca
     1081 ttccgactac aaatctggcc acagcaaacg tattgtccag ctccacatct tccggatcag
     1141 ggggcagcac ctcaatttat catttggcag tcactacaga cagtgccact aatgtgacag
     1201 gactctccaa tcttctcctc cagacaatcg gaaccagcat cacaaacgga acggtcacca
     1261 accttccgac aagcggggga acagaatcca catctgccag ttttcatgtg gttctccaag
     1321 tgacatacag ttcaacagga cctgaagtca taggcgtagg ggtaagcaca acagccaact
     1381 attcggcaaa tgaagctctt ctgactacat ttttggacgg atcaaacatt gcgagcggaa
     1441 cagtcactaa aattgccaag acagacagtt tcagcggagc agctgatcca aaggcagatt
     1501 tcgtctgggt ggttgacaac tcctcttcta tgcagcagga gcaaagtgca gtgtccagcg
     1561 cagcaaccac attcttttca gcactttcca ataagagact cgactacagg ataggggtca
     1621 tcactacaga cagcagtaca ttgagaaccg ccggaggaac aagctgggtt gtcaactcca
     1681 catccggagg tcagacagca ttcgttgcaa atactgcggc tggaacaggc ggaagcggta
     1741 atgaatc
//
LOCUS       DAOQGJ010000924         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49168_length_1746_cov_1.838557, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000924 DAOQGJ010000000
VERSION     DAOQGJ010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49168_length_1746_cov_1.838557"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PK683_13645"
     CDS             <1..460
                     /locus_tag="PK683_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09537.1"
                     /translation="HCSEKKINYTWLYWMFYELIKPPSCPDISKYTQLQKAVTIQAGS
                     AVSVYDRYRIQTSSQNPVSIKIQLKSDCVLYRDFEYCLNGGYGYASSSNSSSSYATSC
                     DDNSVSLRVPIQGGTGVSETCSLSYGAQIYEINIYTLEPKEGCFYEITYN"
     gene            755..1648
                     /locus_tag="PK683_13650"
     CDS             755..1648
                     /locus_tag="PK683_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09538.1"
                     /translation="MKKTLLNINLLYIFTVYLFSISLLSEESAVQKTNWSIQYAFGSG
                     KTNTEAITERDLYNNTFLVGSVSNSPAANMIPFMKQRNKLVGNSYSFRFSAEYLSSSG
                     RFGFITGLTGTEMDFTVEQNGLRRLGLLNLILPRQNSLFGLTPLPQSAFMTLLDIFPV
                     YRSAMNRDTVSYSVPYMDFGLTVHILPRRLLDPYFVFGGSGGFCGKDCSARRGFARFG
                     LRLNFERMYFFLEGEKSVHVFRTGKIDSRQIFDESAFPDGPTPCIASVFASFIHCCQP
                     CRPTRNLDPSDPERRYSSARV"
BASE COUNT          485 a          377 c          379 g          505 t
ORIGIN      
        1 gcactgctca gaaaaaaaga taaattatac ttggctctac tggatgtttt acgaactgat
       61 taaaccgcca tcttgcccgg atatatccaa atatacgcag cttcagaaag cagtgaccat
      121 tcaggcagga tcggcagttt ccgtttacga caggtatcgt attcagacat cctcccaaaa
      181 tcctgtcagc ataaaaatcc agctgaaatc agactgtgtt ttatacaggg attttgagta
      241 ttgtctgaat gggggatatg gttatgccag cagcagtaat tcaagttctt cttatgcaac
      301 atcctgtgac gacaactctg tcagtctaag agtgcctata caaggaggta ccggggtctc
      361 agagacttgc tctctatcct atggcgcaca gatatatgaa atcaatatct atacacttga
      421 accaaaagag ggatgcttct atgaaattac ttacaattga tcatattcta gctgtttgga
      481 aaagaatgat agttttcatc tatcttttgt gtatttcttt tttttctgga ttggctgcgt
      541 atccgtttac aaacaggcca gagaattgtg atacaacagg tcaaaacgat tccaacacta
      601 aagctgcagt ctacggagac agtcgaatgg actatatggg gaatattccg ggactgcctc
      661 tgtatgatat ggatttgcgc agtcgagcca ccgattttct attcccggct tttgcagttc
      721 cctgatccct gatcccaaaa aaaaggagaa atttatgaaa aaaacattat taaatatcaa
      781 cttactttat atctttactg tatacttatt ttctataagt ttactatctg aagaatcggc
      841 ggttcagaaa acgaactggt cgattcagta tgcattcggc tccggcaaaa ccaatacaga
      901 agcgattaca gaacgagatc tttacaataa tacattttta gtaggaagtg tttccaattc
      961 tcctgcggcg aatatgattc cgtttatgaa acagaggaac aagcttgtgg gcaattccta
     1021 cagtttccgg ttttcggcag agtatctgag cagctccggt cgctttgggt ttatcactgg
     1081 actcaccgga acggagatgg actttacagt agagcagaat ggtcttcgta gactgggact
     1141 gctgaatctg atccttccta ggcagaattc tctttttggt ctgactcccc ttcctcaaag
     1201 tgcttttatg accctgctgg atatttttcc agtatatcgg tcagcgatga acagggatac
     1261 tgtgagttat tctgttccct atatggattt cggcctgacc gttcatatcc tgcctcgcag
     1321 acttctggat ccatattttg tattcggagg aagcggtgga ttctgcggaa aggactgttc
     1381 tgccagaaga ggatttgccc ggttcggtct gcggctgaat tttgagagaa tgtatttttt
     1441 cctggaaggc gagaagagcg tccacgtttt ccgcacagga aagattgact ccagacagat
     1501 ttttgacgag tccgcttttc cggacggtcc gacaccgtgc attgcgtcag ttttcgcttc
     1561 gttcattcac tgctgccagc catgtcgtcc cacgaggaat ctggatccaa gcgatcccga
     1621 gagaaggtac tccagtgcgc gtgtgtgaca cacacacaca cacacacaca cacatatata
     1681 tatatatata tacatacaca tacaacacgc acaccatgct gcaaggaaaa gaaagagaga
     1741 gtgatg
//
LOCUS       DAOQGJ010000925         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49211_length_1745_cov_2.037870, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000925 DAOQGJ010000000
VERSION     DAOQGJ010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49211_length_1745_cov_2.037870"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PK683_13655"
     CDS             complement(<1..691)
                     /locus_tag="PK683_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09539.1"
                     /translation="MKKIVKYSFFLIILTVFQACQAGKNSELLESFFHLAYLNKKSTI
                     SGKATKGPIKNALVKIIPLRTNGECKSSDRSIAENTTDSSGNYSVEFQKTGKPVCVFV
                     VPYSANTKMYDEASKKDLTWTDSSFYSLTVMKEPDTSKKKINITPFSRIAASRITELL
                     KTNTDSSKLEAQVVRGNKEATIRFNLNKKYTAKSVRTKQSSVTGRNVTISKNRRNFLK
                     QFKSVSDKDYPD"
     gene            929..>1745
                     /locus_tag="PK683_13660"
     CDS             929..>1745
                     /locus_tag="PK683_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09540.1"
                     /translation="MKFLLFSISFLFIQAIASAKEAVILGTLIGSGQYLETDKGDTYA
                     LQHSDLARELTEITGKKVRILCEIEGINCTPVRYEIAPFASETNLPKWTVKPIPRYVF
                     KGMTAFNPTVSPDGSVLFWTVLLEQHGHSTQKIWYSELDQYGFWKKGVQMDAPLNNKA
                     PSAVIASMPGGNELFVFGSYSDQEIYMDLKKKMDHEKTELLKASKTPKEFETKYALLK
                     EQYKKDLEKLQNRVPLYKSFKQSKGWSIPEVINFPDFYNMYRSEDNHMLQVFGG"
BASE COUNT          518 a          350 c          351 g          526 t
ORIGIN      
        1 gatcaggata gtccttgtca ctgaccgatt taaactgttt cagaaagttt cttctgttct
       61 ttgaaatcgt aacatttctt cccgtaacag aactctgctt tgttctgaca gacttggcag
      121 tatatttctt gttcagattg aagcgaattg tagcttcctt gttgccccgc acaacctgtg
      181 cttccagttt gcttgaatca gtattcgttt tcaaaagttc ggtaattctg gaagctgcga
      241 ttctgctgaa aggagtgata ttgattttct ttttggaagt atccggttcc ttcatcacag
      301 tcagtgaata gaagctgctg tccgtccaag ttaaatcttt tttggacgcc tcatcgtaca
      361 ttttcgtgtt tgcagaataa ggaaccacaa atacgcatac cggttttcct gttttctgaa
      421 attccacaga ataattcccg gaagagtctg tagtattttc tgcaatggat ctgtccgagg
      481 atttgcactc accattggtc cgaaggggaa taatttttac gagtgcattt ttgatcggtc
      541 cctttgtggc ctttccggaa attgtacttt ttttattcag gtacgccaga tgaaaaaaag
      601 actccagaag ttctgaattt ttccctgcct gacaagcctg aaaaacagtc aaaatgatta
      661 gaaaaaagga atatttcact atctttttca tataaacggg cacctcggac acctaatatt
      721 tattggactc ccctttttta tgagtcaagt ccatatttta taaaaattcc caaaaaactg
      781 agagttctat ctcataaaca agcaaaaatc agacctttct caaaaggcaa agttttttat
      841 tgcgaatccg ttcccttgca aaaagctgtg aacagctctg caagagcaca aaaattaaga
      901 tttttggtga ttttccgaaa ggtttagtat gaagttttta cttttttcaa taagtttttt
      961 atttattcag gccattgcct cagctaaaga agctgtgatt ttaggaacac tgatcggatc
     1021 gggtcagtat ctggaaacag acaagggcga cacttacgct ctgcagcatt cagacctggc
     1081 aagagaactc acagaaatta ccggcaaaaa agtacggatt ttatgtgaaa ttgaagggat
     1141 aaactgcacg ccggtcagat atgaaattgc accgttcgct tccgaaacaa atcttcccaa
     1201 atggacagtc aaaccgattc cccgatacgt tttcaaggga atgaccgcct ttaatcctac
     1261 tgtctcgccg gacggaagtg tacttttttg gacagtgctc cttgagcagc atggccacag
     1321 cactcagaag atctggtatt cagaactgga ccaatatggc ttctggaaaa aaggagttca
     1381 gatggatgct cccttgaata ataaagcgcc gtctgcagtc attgcatcta tgccgggggg
     1441 aaatgaactt tttgtattcg gaagttattc agatcaggaa atttatatgg atctgaaaaa
     1501 aaagatggac catgaaaaaa ctgaactgct taaagcatcc aagactccca aggaatttga
     1561 gacaaagtac gctcttctga aagaacagta taaaaaagac ttggaaaaac tccagaacag
     1621 ggttcctttg tacaagagtt tcaaacagag caagggctgg tcaatccctg aggtcatcaa
     1681 ttttcctgat ttttacaata tgtacagaag tgaagacaat catatgctcc aggttttcgg
     1741 cggtt
//
LOCUS       DAOQGJ010000926         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49328_length_1742_cov_2.922940, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000926 DAOQGJ010000000
VERSION     DAOQGJ010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49328_length_1742_cov_2.922940"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PK683_13665"
     CDS             <1..939
                     /locus_tag="PK683_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNH09541.1"
                     /translation="HMYVNLRILNPRFKSQDKSYLINDLNEDMRKAVDKHLDKLVKKT
                     DLLEEVKLQMEKRTQLKELEDAQKGLRRASRNSIPKLMPPTGRTNEPGRVLFIAEGDS
                     AIAGLRPARNPKLHGLFPLRGKPMNCKGMSLAKALQNEELKNIVAIVGLPLDGKVKSI
                     SDLNYERISIITDADFDGYAIRSLMLSFFFEYWPELFELGMIHFSSAPLYEVEVKWKD
                     GKKETIFCIDDKEYNALMDRLKKNGGDMVRKKRNKGLGETSKEAMKFAVDECMIRITL
                     KNKKSADNMQLLWFHKDYAEQRREAISEYAMSVVED"
     gene            948..>1742
                     /locus_tag="PK683_13670"
     CDS             948..>1742
                     /locus_tag="PK683_13670"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNH09542.1"
                     /translation="MHGSLLEWFHSSKRDLPFRKDRTAYTVWVSETMLQQTRVEAMTE
                     KYLSFIKKYPDLNSLASSSDEDIAGAWKGLGYYSRALNLRKGAQYVRDRFGSVFPDNL
                     EDALTVPGVGPYTARAVLSLACGKKYAVLDGNVKRVLSRFFSYEKNIKASGAEKDLQK
                     LADDFLNHDDPGNHNEALMELGASVCGRKPQCGICPLRRECSAFQKGIQERIPLSEKE
                     KKKIRLKMTFIFFVREGNILLVRDRKRRFFRTVCSLPYIAEPEGGEN"
BASE COUNT          528 a          366 c          440 g          408 t
ORIGIN      
        1 catatgtatg tgaatctgag gatactcaat ccgagattca agtctcagga caaatcctat
       61 ctcatcaacg atcttaacga ggatatgaga aaggctgtag acaaacatct tgacaaactg
      121 gtaaaaaaga ccgatctgct cgaagaagtg aagcttcaga tggaaaaaag aacccagctg
      181 aaggaacttg aggatgcgca aaaggggctt cgcagagcct cacggaacag cattcccaag
      241 cttatgcctc ccacaggccg tacaaacgag cctgggaggg ttctattcat cgctgagggg
      301 gattctgcga tcgccggtct ccgtcctgcc agaaatccaa agctccacgg actttttcct
      361 ctccggggaa aaccgatgaa ctgcaagggg atgtcactgg ccaaggctct tcagaatgag
      421 gagctcaaga acatcgttgc aattgtagga cttcctctgg acggaaaggt aaaatcgatt
      481 tcagatctga actatgaaag aatcagcata atcactgatg ccgactttga cgggtatgca
      541 atcagaagtc ttatgctctc gtttttcttt gaatactggc ctgaactttt tgaactcgga
      601 atgattcatt tttcatctgc ccctctctat gaggtggaag tgaaatggaa ggacggaaaa
      661 aaggaaacca tattctgcat cgacgacaag gaatacaatg ctctcatgga caggctgaag
      721 aagaacggcg gggatatggt cagaaagaag cgcaacaaag gactcgggga gacatccaag
      781 gaggccatga agtttgcagt ggatgaatgc atgatcagga tcactctgaa aaataaaaaa
      841 agtgcagaca atatgcagct cttatggttt cacaaggact atgcggaaca gagaagagag
      901 gcaatttctg aatatgcaat gtcggtagtg gaagactgaa aaaatcagtg cacggatctc
      961 ttctggaatg gtttcattct tccaaaagag atcttccttt ccgcaaagac agaacagcct
     1021 acacagtctg ggtgagcgaa accatgctgc agcagacccg cgtggaggca atgacggaaa
     1081 aatatctttc cttcattaaa aaatatccgg atctgaactc tcttgcctcc tcatcggatg
     1141 aggatatcgc cggtgcctgg aaaggtctgg gctactattc cagggcgctg aatctgagga
     1201 agggagcaca gtatgtcagg gacagattcg gttccgtttt tccggacaat ctggaggatg
     1261 cactcactgt tccgggagtc gggccttata ctgcaagagc agttctttcg cttgcctgcg
     1321 gaaaaaaata tgctgttctt gacgggaatg tcaaaagagt cctgtccaga ttcttctcct
     1381 acgaaaaaaa tataaaggct tccggtgcgg aaaaggatct tcagaaactg gctgatgatt
     1441 tcctgaatca tgatgatcct ggaaatcata atgaagcttt aatggagctc ggggcatctg
     1501 tctgcggaag aaaacctcag tgcggcatct gtcctctgag aagggagtgt tcagcatttc
     1561 agaaagggat tcaggaaaga atacctctat ctgaaaagga aaaaaagaaa atccggctga
     1621 agatgacttt catttttttt gtcagggaag gaaacatcct cctcgtgagg gacagaaaaa
     1681 gacgcttctt ccggactgtc tgcagtcttc cgtatattgc agaaccggaa ggcggagaga
     1741 at
//
LOCUS       DAOQGJ010000927         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49334_length_1742_cov_2.662122, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000927 DAOQGJ010000000
VERSION     DAOQGJ010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49334_length_1742_cov_2.662122"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK683_13675"
     CDS             <1..220
                     /locus_tag="PK683_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09543.1"
                     /translation="LFSNRLNNALSLLLSACMLMGIFREGFDMIMALHFSSLSLKEIL
                     QALSFLIFILPYAAALKKLLVISRGETG"
     gene            217..609
                     /locus_tag="PK683_13680"
     CDS             217..609
                     /locus_tag="PK683_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09544.1"
                     /translation="MNLQYRFIQGTAALLGLMYLKSGYDFIITKQIEFIHYTAISSLE
                     IWTVRNLGARLLAISVGFLAALFLNDRRMLALMFLVRLTADIGDFFNSMNTAGLDSFV
                     VPVLGAFVCIETLCLLMLMKIIAQEGKK"
     gene            606..1487
                     /locus_tag="PK683_13685"
     CDS             606..1487
                     /locus_tag="PK683_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH09545.1"
                     /translation="MKDKIVLITGSTGGIGFQTALTLASMNAFVVLHGRSQESGAQAL
                     LEMLQKSGNSQGDFISADLSSISGVRSLSADFQKRFGRLDVLIHNAGSAADTLRKTED
                     GLEFNFAVNAVVPYLLSEMLLKSLKKSKDPRIVTLTGGDLPGKLDLENLQCEKSFLGL
                     DSYSSSKITMMCIMKKYSEILEPKGITCNICYPGYASTSMTQNVTKEMLPPSFRFIFP
                     IFQYFVKPDGGKSAQKASRSTVYLASSDDFSKKTGIYCDKNLRIREMPKPVMDENVTN
                     SVWEYLQRVLSVLDVLS"
     gene            complement(1451..>1742)
                     /locus_tag="PK683_13690"
     CDS             complement(1451..>1742)
                     /locus_tag="PK683_13690"
                     /inference="COORDINATES: protein motif:HMM:NF021498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HNH09546.1"
                     /translation="HGLEILSKYREIFPQTESFLMDRETFETFSEYAVFEKYERKIST
                     DHLTEEERELADNLRNRQESNRLEQEKIPYSYLEEKINHFFHDKTSRTDNTR"
BASE COUNT          471 a          327 c          374 g          570 t
ORIGIN      
        1 tctcttcagc aaccgtctga acaacgctct ttcactgctt ctttccgcct gtatgcttat
       61 gggaattttc agagaaggct ttgatatgat tatggcactt catttttcaa gtttatcctt
      121 aaaggaaatt ctgcaggcac tttcctttct tatatttatt ctgccttatg cggctgctct
      181 gaaaaaactg cttgtgattt caagaggaga aaccggatga atctgcaata ccgatttatt
      241 caaggaactg cagcacttct cggtctgatg tatctgaaat cgggttatga ttttatcatt
      301 acaaagcaga tagaatttat tcattacact gcaatttctt ctctggaaat ctggactgtc
      361 cgaaatttag gtgcacggct tctggcaatc tctgtcggtt ttttggctgc actttttctg
      421 aacgacagga gaatgcttgc actgatgttt ttggtaagac ttacggcaga tatcggtgat
      481 tttttcaatt caatgaatac tgcaggactg gattcttttg ttgttcccgt gctgggagca
      541 tttgtatgta ttgaaacttt atgtctgctt atgttaatga agatcattgc ccaagagggg
      601 aaaaaatgaa ggacaaaatt gttctcatta ctggttctac aggcgggata ggctttcaga
      661 cagctctgac tcttgcatca atgaatgcat tcgttgtttt gcatggacgc agtcaggaaa
      721 gcggtgctca ggcattgctg gaaatgcttc agaaatcagg caacagtcag ggagatttta
      781 tttccgctga cctgtcttcg atttcagggg tccgcagctt gagtgcggac tttcagaaaa
      841 gatttggaag actggatgtt ctgattcaca atgcaggatc tgcagcggac acccttcgaa
      901 aaacagagga cggactggaa ttcaattttg ctgtgaatgc tgttgtcccg tatctgcttt
      961 ctgaaatgct tctcaagagt ctgaaaaaaa gtaaagatcc gaggatagta actttgacag
     1021 gtggagatct tcccggaaaa ttagatttgg aaaatctgca gtgcgaaaaa tcctttcttg
     1081 gtctagactc ttattccagt tccaagataa cgatgatgtg tatcatgaaa aaatattcag
     1141 aaattctgga accgaaaggt atcacctgca atatctgtta tcctggttat gcaagcactt
     1201 caatgactca gaatgttact aaggaaatgc tgcctccgtc atttcgtttc atatttccta
     1261 tatttcagta ttttgtaaaa ccggacggag ggaagtctgc acagaaggct tctcgttcaa
     1321 ctgtatatct ggcgagttca gacgatttca gtaagaagac tggaatttat tgcgataaaa
     1381 atctgagaat tagggaaatg ccaaaaccag tgatggatga aaatgttaca aattcagtct
     1441 gggagtatct tcagcgggta ttatcagttc tggatgtttt atcatgaaaa aaatggttaa
     1501 ttttttcctc gagatatgag tatggaattt tttcctgttc aagcctgtta gattcctgcc
     1561 gatttctcag attgtctgcc agttctcttt cttcttcagt cagatgatcc gtactgattt
     1621 ttctttcgta tttctcaaaa actgcatatt ccgaaaaagt ttcgaatgtt tctctgtcca
     1681 tcaggaacga ttctgtctgc ggaaaaattt cccggtattt cgaaagaatt tccagaccgt
     1741 ga
//
LOCUS       DAOQGJ010000928         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49439_length_1740_cov_3.582789, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000928 DAOQGJ010000000
VERSION     DAOQGJ010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49439_length_1740_cov_3.582789"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="PK683_13695"
     CDS             <1..1666
                     /locus_tag="PK683_13695"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HNH09547.1"
                     /translation="RSQKKADTDTKVQNESVGKKLAELLNRTPSDLHSLDGLRVRRVL
                     PGKEKFGADVISLETNTNFGHIHSLQRIVAGFVQKAYNYHEDDSYLIASYILYYNAYH
                     REDMEYIQGKYVPEISASVSGSKLGLSRNYEYWPGRTQIIIPIEPNILKKTNKDVTLD
                     ELAEINPLLDKSKADEKKKLADLSREKLKDEKNQLKDRKEIVKIEEAELDIAKRRIDE
                     NLEILKKDPLANKDRIAELEKEKTILLKKEIKLTEVKEGVKDKEDRIAKKEGKTPAKE
                     TAESKKEIPKKEVKEEKTAEVPVKKETVEVPEKKVPETVAEPVKTTEPAEETKDAVAL
                     KKEEKEPEDDKEKIIIENPETLKKLEEIAKELEAKKEEEKKKQEFSDNVFSGRIPFIK
                     PVTLNEGQCSNELHILDPAKDDFIYKSEFNRICGRNFQIFNGMILVVGGFQEASGDVR
                     LVLLGSRNLARAGNSEVKVYIKTHMEVNGEFLYAVEQDKGKYYYARFDKNLKREVRTS
                     AEINPDSTIVFYGRKIYIYGKSPDTGNIEIKVFDRETLKFLKKTQS"
BASE COUNT          650 a          299 c          425 g          366 t
ORIGIN      
        1 aagatcgcag aagaaggcag acacagatac gaaggttcag aatgagtctg tcggaaagaa
       61 gcttgcagaa ctgctgaaca gaacaccttc cgatcttcat tcacttgacg gattacgagt
      121 cagaagagtt cttcccggaa aggaaaaatt cggtgcagac gtgatcagtc tggaaacaaa
      181 tacaaatttc ggtcatattc attctctgca gagaatcgtg gccggttttg tgcagaaggc
      241 ttacaattac catgaagacg attcctatct gattgcctcg tacatactgt actacaatgc
      301 gtatcacaga gaggatatgg aatatataca gggaaagtat gttcctgaaa tttcggcatc
      361 cgtatccgga agcaaattag ggctctccag gaactatgag tactggccgg gcagaacaca
      421 gatcatcatt ccgattgaac cgaacattct gaaaaagaca aacaaggatg tcactctcga
      481 tgaacttgct gaaatcaatc cattgcttga taaatcaaag gcagacgaaa agaagaaatt
      541 agccgatctg agcagagaaa aactcaagga tgaaaagaat cagcttaaag acagaaagga
      601 aattgtcaaa atagaagaag ctgaactgga tattgcaaaa cggagaattg atgagaatct
      661 ggagattctg aaaaaggatc cgcttgcaaa caaagacaga attgctgaac tggaaaagga
      721 aaaaacgatt cttctgaaaa aggaaatcaa actgaccgaa gtcaaggaag gggtgaagga
      781 caaggaagac aggattgcca aaaaggaagg aaaaactcct gcaaaggaaa cggctgaatc
      841 gaaaaaggaa attccgaaga aagaagtgaa ggaagagaaa actgccgagg tccctgtcaa
      901 aaaagaaaca gtggaagtcc ctgaaaaaaa agtccctgaa actgtcgcag aaccagtcaa
      961 gacaacggag ccagcagaag agaccaagga tgcagtcgca ctgaagaagg aagaaaaaga
     1021 gcctgaggac gataaggaaa aaatcattat agaaaatcct gaaactctga agaagcttga
     1081 agagattgca aaggaactgg aagccaaaaa agaggaagaa aaaaagaagc aggagttttc
     1141 ggacaatgta ttctccggac gaattccgtt tatcaagcct gtgactctga atgagggaca
     1201 gtgcagcaat gaactgcata tcctcgatcc agcgaaggat gattttatct ataaaagtga
     1261 attcaacaga atctgcggac ggaatttcca gatattcaac ggaatgattc tggttgtagg
     1321 cggctttcag gaagcatcag gagatgtcag actggttctt ttaggaagca gaaatcttgc
     1381 ccgtgccggg aattcagagg tcaaggtata cataaagacg catatggaag tgaacggtga
     1441 gtttctgtat gcagtggaac aggacaaggg aaaatactat tatgccaggt ttgacaaaaa
     1501 cctgaaaaga gaagtccgga caagtgcaga gattaatccc gattcaacga tagtatttta
     1561 cggcaggaaa atttatattt acggaaaatc gccggatacc ggaaatatag agattaaagt
     1621 atttgacagg gagactttga agtttctcaa aaaaactcag tcttgattca ggtggaaaat
     1681 ggatataaca acagcgtcta agattctgga caagggtctg tctttaggtt ttgattatac
//
LOCUS       DAOQGJ010000929         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49450_length_1740_cov_2.588131, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000929 DAOQGJ010000000
VERSION     DAOQGJ010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49450_length_1740_cov_2.588131"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..>1740
                     /locus_tag="PK683_13700"
     CDS             274..>1740
                     /locus_tag="PK683_13700"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09548.1"
                     /translation="MNDAQNLKSEFMEWYIGRSEKTDGYFRRIFHGKPEKLESELDEY
                     CEFFDFSIFDIGENNYKKYIKDIQIAISDTESDFFKYSRKKGHHRPKFILGNENYLKF
                     LKEKFRKKTLRKQNTERHHQDERPLNLILYGPPGTGKTYNALIEAVKIANPSFNTDRD
                     RKEVKAEFDRLRKEGRIVFTTFHQSMSYEDFIEGIRPIEPENRGQPVIYRVIPGILKE
                     FCDRIRSAEKRAEKAESEESRKSAEFNYLYSAFIYKLNRTVSQLKEGEFHYLESRRGK
                     VKILRMQEDSFLTLDESADATETVSREKLQKIYERFSSPDEILQILEQVKTVGTDSGW
                     SNNSYAAFIALKAFEISYRADAERMKRNAKLNYAIIIDEINRGNVSQIFGEIITLIEE
                     DKRLGREESLEVTLPGSREKFGIPASLFIIGTMNTADRSVEALDTALRRRFSFRETLP
                     ESEIIAERGRLKDSGGNLNGISLPELLDTMNRRIERLLD"
BASE COUNT          635 a          317 c          388 g          400 t
ORIGIN      
        1 ctgcattagc tgcctctttt ttacggccgt caatgctgta tccgtacatg agctcagctt
       61 atgtgccgcc ctgagaaatc aataaattaa atacatcttc tgcctatttt atgcacagaa
      121 tttaacagca ctgattatat tgatctttca tttaaacgtt gattaaaaag gaaccggtca
      181 tttttactga atgtaaagca ataaaagaaa aatctgtccg tgagacggca aaaagcgttg
      241 tcctccggca cagcacagac agggaattca gttatgaatg atgcacagaa tctaaaatcg
      301 gaatttatgg aatggtacat cggcagatcg gaaaaaacag acggatattt cagaaggata
      361 tttcacggaa agcccgaaaa gctggaatca gaactggatg aatactgtga attctttgat
      421 ttcagcatat ttgacatcgg agaaaataat tataaaaaat atataaaaga tatacaaata
      481 gcaatttcag acactgaaag tgattttttt aaatacagca gaaaaaaggg acatcaccga
      541 ccaaaattca ttcttggaaa tgaaaactat ctgaaatttc tgaaggaaaa attccggaag
      601 aaaactctgc ggaaacagaa cactgaaaga catcatcagg atgaaagacc tttaaatctg
      661 attctgtacg gacctccggg aacaggaaaa acatacaatg cgcttattga agctgtaaaa
      721 atcgcaaatc catccttcaa tacagaccgg gacagaaagg aagtcaaggc agaatttgac
      781 agactcagaa aagaaggaag gatcgtattt acaactttcc atcagagtat gagctatgaa
      841 gatttcattg aaggaatcag gccgattgaa ccggaaaaca gaggacagcc ggtcatttac
      901 agggtgattc cgggaatact gaaggaattc tgcgacagaa tcagaagtgc agagaaacgt
      961 gctgaaaagg ctgaatcaga ggaaagcaga aaatctgcag aatttaatta tctttacagt
     1021 gcttttatat acaaactcaa cagaacagtt tcgcagctga aggaaggaga attccattac
     1081 ctggaatcca gacggggaaa agtaaaaatt ctgaggatgc aggaagacag ttttctgact
     1141 ctggatgaat ccgcagatgc gactgaaaca gtcagcagag aaaaactgca gaaaatctat
     1201 gaacggttca gcagtccgga tgaaatactg cagatactgg aacaggtgaa gacagtcgga
     1261 accgattcag gatggtcaaa caattcctat gcggcattca ttgcactgaa agcgtttgag
     1321 atttcctaca gagcggatgc agaaaggatg aaaagaaatg caaaacttaa ttatgcaata
     1381 attatagatg aaatcaaccg tggaaacgta tctcagattt tcggagaaat cattacactg
     1441 attgaggaag acaagagact gggaagagaa gaatccctgg aagtgacact gccggggagc
     1501 cgggaaaaat tcggaattcc tgcttcactt ttcatcatag gcacaatgaa cacggcagac
     1561 agaagcgtgg aagcacttga tactgctctc agaagacgtt tcagtttcag agagacactg
     1621 cccgaatcag aaatcattgc tgaaagaggc cgactgaagg actcgggagg aaatctgaac
     1681 ggaatcagtc tccccgaact tcttgataca atgaacagaa gaattgagag actgctcgac
//
LOCUS       DAOQGJ010000930         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49539_length_1738_cov_2.915033, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000930 DAOQGJ010000000
VERSION     DAOQGJ010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49539_length_1738_cov_2.915033"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PK683_13705"
     CDS             <1..820
                     /locus_tag="PK683_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09549.1"
                     /translation="PNITMTGNTLDSGSPSGVTPNRYALLLNSGGTGWANGSSIQRNT
                     FISRPGTGYSATVSITPSTATGNSLLFCSNVMIGGNNTGPSSSTVLEIGNATSVGNKF
                     MGNTYIGGTVSGGVSVLHRIMDSTVKHNLDLNLFAGDSSVSALTTCVSEAVGDATYAT
                     LERNNFSGCNTLYSNNAGATLMTITCSGNVGIIGCAAPPNTLDFPTGHFNQNAAPIFN
                     NPSGMDYHLNASTTSVIYSGTIAADITAFNAGCGNSLDRDGNTRSAGTALGAFK"
     gene            complement(813..1415)
                     /gene="fabR"
                     /locus_tag="PK683_13710"
     CDS             complement(813..1415)
                     /gene="fabR"
                     /locus_tag="PK683_13710"
                     /inference="COORDINATES: protein motif:HMM:NF008402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HNH09550.1"
                     /translation="MNQRYAQKMKTRSTLLQSALKLMGEEKALGSLSLREVAAEAGIV
                     PAAFYRHFKDMEELGLALVDEVSAKLRSILRDARKKGAYKTALKISIDMFFSYVKQNR
                     LLFRFMVRERVGGNRRIRQAIKSELNFVASELASDMRIKRVPPSELAFVAEFIVFTSF
                     GNVSEFLDSDPHDTKTENFIKAKTVKQIRTIFRGSVIKII"
     gene            complement(1422..>1738)
                     /locus_tag="PK683_13715"
     CDS             complement(1422..>1738)
                     /locus_tag="PK683_13715"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HNH09551.1"
                     /translation="GAIILSLQKILENFKKDPQDVMNSRHFLSYSLDSLVKILSKYIE
                     LTKSESSKKQFEATLEKVLPTLDSIHTAIDGHYNKLLENDVMELDAEIQVMEKTIQMG
                     GF"
BASE COUNT          477 a          376 c          370 g          515 t
ORIGIN      
        1 tccaaatatc actatgacag gcaatacatt ggattcaggc agtccaagcg gagtcactcc
       61 caacagatat gctcttctgc tgaacagcgg cggcactggc tgggccaacg ggagcagtat
      121 tcagagaaat actttcatca gcagaccggg aaccggttac tctgcaacag tgtcaatcac
      181 tccttctacg gctacaggaa attcgcttct tttctgcagc aatgtaatga tcggtggaaa
      241 caataccgga ccgtccagtt ccactgtttt ggaaatcggg aatgcgacca gtgtgggaaa
      301 taaatttatg ggcaatactt atattggcgg tactgtatcc ggaggagtga gcgttcttca
      361 cagaattatg gacagcacag tcaaacataa tctggatttg aatctgtttg cgggtgacag
      421 ttctgtttcg gctctgacaa cctgtgtctc cgaagcagta ggcgatgcga cttatgcaac
      481 tttggaaaga aataatttct ccggctgcaa tacattgtat tccaataatg caggtgcaac
      541 tttaatgaca attacctgca gcggcaatgt gggaattatc ggttgtgcgg ctcctcctaa
      601 cactcttgat tttccgacag gacatttcaa tcaaaatgca gctccaattt tcaacaatcc
      661 atcagggatg gattatcatc tgaatgcatc cacaacctca gtgatttatt caggaacaat
      721 tgctgcagat attactgcat ttaatgccgg gtgcggaaac agtcttgaca gggacggaaa
      781 tacgaggagt gcaggaactg cacttggagc atttaaataa ttttaattac cgaaccgcga
      841 aaaatggttc tgatctgttt gactgtcttt gctttaatga aattttctgt ttttgtatcg
      901 tgcggatctg agtccagaaa ttcgcttaca tttccgaagg aagtgaatac aataaactcg
      961 gccacaaatg caagttccga aggaggcact cttttgatcc tcatatcaga ggcaagttcg
     1021 gatgcaacga aattcagctc acttttgatt gcctgacgga ttcttctgtt tccgccgact
     1081 ctttccctta ccataaaacg gaaaagaagc ctgttctgct tgacataact gaagaacata
     1141 tcaatggaaa ttttcagtgc agttttatat gcaccttttt ttctcgcatc tctcagaatg
     1201 cttctgagtt ttgcggaaac ctcgtccaca agggcgagtc ccaattcttc catatcctta
     1261 aaatgtctgt aaaatgctgc cggaacaatt cctgcctcag cagcgacttc tctgaggctc
     1321 aaggaaccga gggctttttc ttctcccatc agttttaatg cagactgaag cagagtgctt
     1381 ctggtcttca ttttctgggc ataacgctga ttcagcttga tttaaaatcc tcccatctga
     1441 attgtctttt ccatcacctg aatttctgca tccagctcca tcacgtcatt ttccagaagt
     1501 ttgttgtaat gtccgtcaat tgcagtatgg atggaatcga gtgtcggaag aactttttcc
     1561 agagtggctt caaactgttt ctttgaagac tctgatttag taagttcaat gtacttagag
     1621 aggattttca ccagagagtc cagagaatag gaaagaaaat gtctggaatt cattacatcc
     1681 tgaggatctt tcttgaaatt ctcaaggatt ttttgtaatg ataaaataat ggctccag
//
LOCUS       DAOQGJ010000931         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49618_length_1737_cov_1.868609, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000931 DAOQGJ010000000
VERSION     DAOQGJ010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49618_length_1737_cov_1.868609"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK683_13720"
     CDS             <1..199
                     /locus_tag="PK683_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09552.1"
                     /translation="SLKDLILNQAQFRTVYASTRKTELGFSIGFSFPVDVPEQKSEVK
                     PESEKTEEKSDGGKKEKSEKE"
     gene            complement(255..479)
                     /locus_tag="PK683_13725"
     CDS             complement(255..479)
                     /locus_tag="PK683_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09553.1"
                     /translation="MTEKRSSIDLIIEEYKKYVDRTLLEENLKLSYTERVEKLQRLLR
                     TFEELKNAGGRPKDFEAISELEILHEKAEK"
     gene            complement(489..992)
                     /locus_tag="PK683_13730"
     CDS             complement(489..992)
                     /locus_tag="PK683_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09554.1"
                     /translation="MKFIYPVIFYFKLFPILIFGWFKVLLRGLSRLGMYWSSLGRVEK
                     ALFVFLFLQMFFSLRPWLVYKINFTGEAETVKVSAKLNLYFVILSVSAFFISAFYHEK
                     WKKTILGIFQGLNVLLFSAGLLFPSKIFSDFIEKSDYGFSPNLYLFAAFSVLSALSVG
                     WYFKTES"
     gene            complement(989..1447)
                     /locus_tag="PK683_13735"
     CDS             complement(989..1447)
                     /locus_tag="PK683_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNH09555.1"
                     /translation="MSVEKNLEKLGIRLPEAPKAIASYIPAQTAGNLVFTSGQLPLLA
                     GTLQKPGKVGVNITLEEAGECAKIACLNAIAAVHSKIGGISRVRQIVKVGVYVASASS
                     FVEQHKVANYASDFLLELFGEKGRHARFAIGVVSLPLDACVELELTAEFE"
     gene            complement(1538..>1737)
                     /locus_tag="PK683_13740"
     CDS             complement(1538..>1737)
                     /locus_tag="PK683_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09556.1"
                     /translation="STFYIFDKNLNLLDKKSIGESVRVFSGWFVPSNAGIESDKNPPP
                     IAFKPHIQEYYDYLVEFLNKK"
BASE COUNT          549 a          363 c          355 g          470 t
ORIGIN      
        1 gagtctgaag gatctgatcc tgaatcaggc acagttcaga actgtatacg caagcacaag
       61 aaaaacagaa cttggattct ccataggatt ttctttccct gtcgatgttc cggaacagaa
      121 gtctgaagtg aaaccggaaa gcgaaaagac agaggaaaaa tcggatggag gaaaaaaaga
      181 aaaatcggaa aaggaatgat gaaagaaaat gaaattgtat ctttttttat ataaagagta
      241 aaaaagattt ttattcactt ctctgctttt tcgtgcagga tttccagttc agaaatggct
      301 tcaaagtctt tcggccggcc gcctgcattt ttcagttctt caaatgttct taaaagtctc
      361 tgcagttttt ccacacgctc tgtataggac agttttaaat tttcttcgag cagagttctg
      421 tccacatatt tcttgtattc ttctataatc agatcaatag atgatctttt ttctgtcata
      481 gtccaggctc agctctctgt cttgaaatac cagccgacag acagggcaga cagaacggaa
      541 aaagctgcaa agagatagag gttaggagaa aaaccgtagt ctgatttttc aataaaatcc
      601 gaaaagattt tagaaggaaa aagaagccct gcagagaaaa ggagaacatt cagtccctgg
      661 aaaattccca gaatggtttt cttccatttt tcatgataaa atgcagatat gaagaatgca
      721 ctaactgata atatgacaaa gtataaattg agttttgcag agactttgac cgtttccgcc
      781 tcacccgtga aattgatttt gtatacaagc cagggacgaa ggctgaaaaa catctgcagg
      841 aatagaaaga caaagagagc tttttccact cttccgagag acgaccagta catcccaagt
      901 ctggagagac cccgcagaag caccttaaac catccgaaaa taaggatcgg aaatagctta
      961 aagtaaaaga ttacggggta gataaatttc attcaaactc tgcagtcagt tccagttcca
     1021 cgcaggcatc gagaggaagg gaaaccacgc cgatggcaaa tctcgcatgt cttccttttt
     1081 caccgaaaag ttcgagcaga aaatcactcg cataattggc taccttgtgc tgttccacaa
     1141 aagaggaagc agacgccaca tacacgccga ccttcactat ctgtctcact ctcgaaattc
     1201 cgccgatctt gctgtgaacg gctgcaattg cgttcagaca ggcaatttta gcgcattctc
     1261 cggcctcttc cagggtgata ttgaccccta ccttgccggg tttttgcagc gttcctgcca
     1321 gaagagggag ctgaccggat gtaaaaacca gatttcccgc agtctgtgca ggaatatacg
     1381 atgcaatcgc cttcggtgct tccggaagac gaatcccaag tttttccaga ttcttttcta
     1441 cactcattct gattccagaa tttttacttc cggtatattg gaaattagtt tttgtgcaga
     1501 atgccaaaaa gatttacgga tttcggggca cagcccttca tttcttattt aaaaactcca
     1561 caagataatc ataatattcc tggatatgcg gcttaaacgc aatcggagga gggtttttat
     1621 cagattcgat tcctgcattg gaaggaacaa accacccgga aaaaactctc actgattcac
     1681 cgattgattt tttatccaga agattcaaat ttttatcaaa aatataaaag gtggaac
//
LOCUS       DAOQGJ010000932         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49641_length_1736_cov_3.607377, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000932 DAOQGJ010000000
VERSION     DAOQGJ010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49641_length_1736_cov_3.607377"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK683_13745"
     CDS             <1..364
                     /locus_tag="PK683_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09557.1"
                     /translation="GRQILFDGRPLNSLSAYSPRNYPWMLTGSDLADTSLHNDDFVNS
                     LAYLGRLFNGPGIVSFSNQADTHTAFFLKSLLKKNPSKVSLFERYTDAFKETQLNMEE
                     DRFWLGWKPYTNVLISSQ"
     gene            406..1701
                     /locus_tag="PK683_13750"
     CDS             406..1701
                     /locus_tag="PK683_13750"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNH09558.1"
                     /translation="MDPLNIEFDRKEIIRNEIERENRIVSEPEERLAKYADMLVSPFN
                     FYRATAHLFFRDIGAGVVQIPSEWKNSKESYTWIQGDLHSQNLGYFDNSLGEVKFDLN
                     DFDESYIGPSYWDLIRFMSALYLQREELDFECSVSDTDELALKFLEEYQETMEKIQED
                     TKYLERELTEEYLNGFLKDRIKDLQFQGKKKTAVKKWCTEENGKLIFDLQNIDLRKLD
                     KIVWDKIYSTVSVYSVNLKEKYQVQDITRRINSGLGSQGMKKYYILMKKNKDEQFIIA
                     ELKEQKSPSMFLAGEISEKEYSEKYKSHSERSAAAMNSILKNADSFVAALKIDGLDFI
                     IKKISDYKTKFKPEEFSDKSDFKNFIKYSAKATAIAHCRAQRYVPSRINTSFEDSVLN
                     YLNKWPDIKDHLCVVSRTYADQVFSDFVLFKELISEKSL"
BASE COUNT          577 a          296 c          353 g          510 t
ORIGIN      
        1 gggaaggcag attctgtttg atggccgtcc gctgaattca ctttctgcgt attcacctag
       61 aaattatccg tggatgctta ctggatctga tcttgccgat acatcacttc ataatgatga
      121 ttttgtgaac tctcttgcat atctgggcag actgtttaac ggcccaggaa ttgtttcatt
      181 ctcgaaccag gcagatactc acactgcatt ttttcttaag tcacttctga aaaagaatcc
      241 atctaaagtc agtctttttg aaagatatac agatgcattc aaggaaactc agctgaatat
      301 ggaagaagac agattctggc tcggatggaa accgtatacg aatgttttga tcagcagtca
      361 ataattatta ttgaatcatc tgcagtgata tgattcattg tacaaatgga tcctttgaat
      421 attgagtttg acagaaaaga aatcatcaga aatgaaattg aaagagaaaa ccgaattgtt
      481 tcagagccgg aagagagact tgcaaagtat gcagatatgc tcgtttcccc tttcaatttt
      541 tacagagcca cagcacattt attcttcagg gatataggtg ccggtgtagt tcagattccc
      601 tctgaatgga aaaattctaa agagtcttat acctggatac agggtgatct gcattcacag
      661 aatctgggat attttgacaa ctctctgggt gaagttaaat ttgatctgaa tgattttgat
      721 gaatcttata ttggaccgtc ttactgggat ctgattcgtt ttatgtctgc tctgtacctg
      781 caaagagaag aactggactt tgagtgttct gtatccgata ccgatgaact tgcattgaaa
      841 tttctggaag aatatcagga gactatggaa aaaatacagg aagatacgaa gtatcttgaa
      901 agggagctta ctgaagaata cttgaacgga tttctcaaag acaggattaa agatctgcag
      961 ttccagggca aaaaaaagac tgctgtaaaa aaatggtgca cagaggagaa tggaaagctg
     1021 atctttgact tgcaaaatat cgacctgaga aaactggata agatcgtatg ggataaaatt
     1081 tattcaacag tatctgtcta ttctgtgaat ttgaaagaaa aatatcaggt gcaggacatt
     1141 accagaagaa tcaattccgg actcggaagt cagggaatga aaaaatatta tattttaatg
     1201 aaaaaaaata aagacgaaca gtttattatt gcagaactca aagaacagaa atccccatca
     1261 atgtttttgg ctggagaaat ttccgaaaag gagtattcag aaaaatataa gtcacattct
     1321 gaaagatccg ctgcggcaat gaattcaatc ctgaagaatg cagacagttt tgtcgctgca
     1381 ttgaaaattg atggactaga ttttatcatt aaaaaaatat cagattacaa gactaagttc
     1441 aagccggaag aattttcaga taaatcggat tttaagaatt tcatcaaata cagtgccaaa
     1501 gcaaccgcca ttgcacattg ccgtgcacaa aggtatgttc cttcgaggat caatacttca
     1561 tttgaggatt cagtgctaaa ttatctgaat aaatggcctg atattaagga tcacctctgt
     1621 gttgtttccc gcacttatgc agatcaggtc ttttccgatt ttgttctgtt taaagaacta
     1681 atttcggaga aaagcctctg aaaaactgca gattctctct gtcaacccct aaactg
//
LOCUS       DAOQGJ010000933         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49658_length_1736_cov_2.677573, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000933 DAOQGJ010000000
VERSION     DAOQGJ010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49658_length_1736_cov_2.677573"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PK683_13755"
     CDS             complement(<1..340)
                     /locus_tag="PK683_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09559.1"
                     /translation="MDDFFIRMACLEDSEALTEIHCSALPDDLLPKLGKKYIKNVYYS
                     KVLKSKYAITMLLEKGEIPVSFVTFTLEPEKLLIEIKKQKIALLLALIKNSFSQPGIV
                     PSLWNNMNTKT"
     gene            complement(333..1460)
                     /locus_tag="PK683_13760"
     CDS             complement(333..1460)
                     /locus_tag="PK683_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09560.1"
                     /translation="MLSEEVEFLSENLFIIKGTSAGSGGFSLNYDLFWNAFVYMSRLE
                     EFSAEQSGKKIRSYSLAHPRKDKSTFSIPVVNHLFIDLKSLISASFSALKFGRKKKPT
                     IEFSHDIDYIRKTIQLRLKQTAFNSFKTLRSVSRPASFFQNFRKTFSFFLSSPDYWPF
                     DFWEQADAKYNIQSVFYIYAAAGQKNFRSWLIDPSYDIRRDRTVRNALRRLISMGFKA
                     GLHGSYRSAVSEIQLKTEKEILAETSGTEITMTRQHWLNYEENITPYIHEKLFAFDST
                     LGWNDRIGFRSGFASQYRPYDHRNRRPFDYFITPQIIMDSNIYDYGSGRESELENKSV
                     QILENLESFSSSFVSISWHDRVFTEDYGWFPFYERIQKLYG"
BASE COUNT          562 a          335 c          302 g          537 t
ORIGIN      
        1 gggttttggt attcatatta ttccataatg acgggactat accaggctga gaaaagctat
       61 ttttaattag agcaaggaga agagctattt tttgtttttt gatttcaata agcagttttt
      121 ctggctctaa tgtaaaagtt acaaaagaaa caggaatttc tcctttttcc aggagcatag
      181 taattgcata tttagatttt aatacttttg aatagtatac atttttaata tacttttttc
      241 ctaattttgg aagaagatca tcaggcaaag ctgaacaatg aatttcagtc aatgcttcag
      301 aatcttccaa acatgccatt cgaataaaaa aatcatccat ataatttctg aatcctttca
      361 taaaatggaa accatccata atcctctgta aaaactctgt catgccatga tatggaaacg
      421 aatgaactgc tgaaagattc aagattctcc aaaatctgaa cagacttgtt ctcgagttca
      481 gattcccttc ctgaaccata atcgtagata ttcgaatcca ttataatctg tggagttata
      541 aaataatcaa acggacgtct gttcctgtga tcatatggcc tgtattgcga agcaaagcct
      601 gaacgaaagc ctattctgtc attccatcct aaagtagaat caaaagcaaa aagcttttca
      661 tgaatgtaag gtgtgatgtt ttcctcatag ttcagccagt gctgtcttgt cattgttatt
      721 tcagtgcctg aagtttccgc cagtatctct ttctctgttt tcagctgaat ctctgaaact
      781 gcacttctgt aacttccatg cagtccggcc ttaaaaccca tggaaatcag tctgcgaagc
      841 gcatttctga cagttctgtc tctgcgaata tcataagatg ggtcaatcag ccaggaacgg
      901 aagtttttct gccctgcggc tgcataaata taaaagactg actgaatatt gtacttagca
      961 tcagcctgtt cccaaaaatc aaaaggccag taatcaggag aggaaagaaa aaaagaaaag
     1021 gtttttcgga aattttggaa aaaagaagca ggtctggaaa cagatctcag tgttttgaaa
     1081 gaattaaacg cagtctgctt cagacgaagc tgaatggttt tacgaatata atctatatca
     1141 tgagaaaatt cgatggtcgg cttttttttt ctgccgaatt tcaaagctga aaagctggca
     1201 gaaatcagag acttcagatc aataaagagg tgatttacga caggaatcga aaatgtactt
     1261 ttatcctttc tcggatgtgc cagtgaataa cttcggattt ttttgcctga ctgttcagca
     1321 ctgaattctt caagtctgga catatacaca aatgcattcc aaaacaagtc ataattcaga
     1381 ctgaaacctc ccgaaccagc agaagtgccc ttaataataa aaaggttttc agacagaaac
     1441 tctacttcct cggaaagcac ccctgctctg catgggacgg aaacagtttc agcccttaag
     1501 ttttttagat aatatataga acaatcagaa ttaatacttt catcagtatt tacagcttga
     1561 acagaaaact cgcataattc agttctctga aattcatcca gtatatactg cagccatgca
     1621 gtatctttta aatctgtttt tatatataga atcatacagc aaactttctg aaccagactt
     1681 ccatattcaa ggcaccccaa agatctctgc tgaacctttc tgatttccgg atattt
//
LOCUS       DAOQGJ010000934         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49685_length_1735_cov_4.989286, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000934 DAOQGJ010000000
VERSION     DAOQGJ010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49685_length_1735_cov_4.989286"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1267
                     /locus_tag="PK683_13765"
     CDS             236..1267
                     /locus_tag="PK683_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09561.1"
                     /translation="MKWKPENTWVIYASLVEGHFNPDGRRDFEMVEAFKEAGIPEEQI
                     YFLGDHKATGVNMEKALAEVCESADEDSFLIYIYSGHGGLSNLDDGYTCYYALEGEKN
                     FYSADVIKQLDSSFGGKFAWVISDCCYSGAATENVEAYEGEVNIAALSSTAPFDSAWS
                     GWLLIAQLIWLMEGRSFLDEDGDGKITLTDFCTFVENEMALVRHFRSNFFIPEGFEDF
                     AFHSDVEETSHERERERVTAPRGDSWDYARILSVDEDEETAEVQWLDRYGNSTDTVKL
                     KDCSPIEVKQLEAGDKVKVVKADQNNKEGAKGEVEEVFYGLHLVHFEGKETRQEEYDW
                     LDYSKIKKI"
     gene            1267..1713
                     /locus_tag="PK683_13770"
     CDS             1267..1713
                     /locus_tag="PK683_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09562.1"
                     /translation="MFSVEPPDRHTMEVFTDYLQSRAFFLLTEDGKSHPAERRTFSQS
                     QAAVISEKFLSAGKCTGIGRRHGTPMLYHFIITEISDGSARMKYSILESIEDLSRMTE
                     QLFFTASFFQKQKLQTLIFPCIPDFTVKGFQIQRNQDVLIISSDEK"
BASE COUNT          515 a          332 c          428 g          460 t
ORIGIN      
        1 gcatcattcc tcataacatc tttgaacgtc tactgttaat tgtacagcat tggccgacag
       61 gcagccgaag gcagccgaag gcgtaaggtg agctgctcac ttgtaaagaa agtgagaaat
      121 ttcatattat aagtacattt atatttctgg atcataaata aaagctcgcc aaaatccagg
      181 attctccaaa aatgtccgga gtcagatcgg tttaaatagt cagacggaga aatttatgaa
      241 atggaaacct gaaaatacct gggtgattta tgcatcgtta gtggaaggtc atttcaatcc
      301 tgacgggaga agggatttcg aaatggtgga ggctttcaag gaagcaggta ttcctgaaga
      361 acagatttat tttctgggcg atcacaaggc aaccggtgtc aatatggaaa aagcccttgc
      421 cgaagtctgt gagtctgctg acgaggattc atttttgatc tatatttaca gcggacacgg
      481 aggactttcg aatctggatg acggttatac ctgttattac gctttggagg gtgagaagaa
      541 tttttattct gcagatgtca tcaagcagct ggattcttct ttcggcggaa aatttgcctg
      601 ggttatcagt gactgctgct attccggcgc agccacagaa aatgtggaag cttacgaagg
      661 cgaggtgaac atagccgcat tgtcttccac tgctcctttt gacagtgcgt ggtcgggatg
      721 gcttctgatt gcacagctga tctggcttat ggaaggcaga tcatttctgg atgaagacgg
      781 agacggaaaa attactctca ccgatttctg cacattcgtg gaaaatgaaa tggctctggt
      841 gcgccacttc agatcaaact ttttcattcc ggaaggattt gaagactttg catttcacag
      901 tgatgtggaa gaaacttccc atgaaagaga aagggaaagg gtcactgctc cgcgaggaga
      961 ctcctgggat tatgcgcgca tactttctgt ggatgaggat gaagagactg ctgaagtgca
     1021 atggctggac cgatacggga attcaacgga tacagtgaag ctcaaagact gcagcccgat
     1081 tgaagtcaag cagctggaag caggtgacaa ggtaaaagtt gtcaaggcgg atcagaataa
     1141 taaggaaggg gccaaaggag aagtggaaga agtattctat ggacttcatt tggttcattt
     1201 tgaagggaag gaaacaaggc aggaagaata tgactggctt gactacagta aaataaagaa
     1261 aatataatgt tttctgttga gcctccggac aggcatacta tggaagtttt cactgactat
     1321 cttcagtcca gagccttctt tcttctgact gaggatggaa agtctcatcc tgcggagaga
     1381 aggactttct cgcagtccca agcagcagtc atctcagaaa aattcctttc tgctgggaaa
     1441 tgtaccggaa tcggcagaag acacggaacc ccgatgctgt atcactttat cattacagaa
     1501 atatcggacg gttcggcgag aatgaaatat tccattctgg aaagcattga ggatctttct
     1561 cgaatgactg aacagctttt ttttactgca tccttttttc agaaacagaa actccagacc
     1621 ttgatttttc cctgcattcc cgattttaca gtaaaaggtt ttcagataca gagaaatcag
     1681 gacgttctca tcatctcttc agatgaaaag taaaaaagaa aacccagtct tcagc
//
LOCUS       DAOQGJ010000935         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49717_length_1735_cov_2.508333, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000935 DAOQGJ010000000
VERSION     DAOQGJ010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49717_length_1735_cov_2.508333"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PK683_13775"
     CDS             complement(<1..751)
                     /locus_tag="PK683_13775"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNH09563.1"
                     /translation="MTIMTEKERLKGILQIAWPLIIANSFWNLQITIDRIFLGMFSTE
                     ALGAAMAVMGVFWVPMALLQQTAGYVTAFVAQYYGAEENEKIGSSVWQSIHVSIIGGL
                     LFLTLNFASSWFFSLADHSPSVQALEVEYYNSLAFSALPTALVAAVSGFFTGLGKTRT
                     VTLINLTGLILNVILDYALIFGNLGFPKMGTAGAGYATAIATLGAAAFGLALIFSQEN
                     ERLYRIRSSWRPDFGLIRQFLRFGLPSGFQWA"
     gene            866..1129
                     /locus_tag="PK683_13780"
     CDS             866..1129
                     /locus_tag="PK683_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09564.1"
                     /translation="MNDITMISILEKLIPWYENLKVTREEYLDLEEDGFLYDMIEGVL
                     HLSPSLDFKHSDIAGNFYFEVRKYLQNHSVGKAVFETDVLLPE"
     gene            1148..1531
                     /locus_tag="PK683_13785"
     CDS             1148..1531
                     /locus_tag="PK683_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09565.1"
                     /translation="MKGSVIIGMSILGICGCLFISDRFQGYGEDAEYAKLQESWSEYQ
                     GEMNWSDADTKCKGLGMKLASREEFNAARAGDAIGKIRKHGNAFWTSELFGTERAHAY
                     GTAKGSDFIQFKTEKNHVVCIKNKP"
     gene            complement(1528..>1735)
                     /locus_tag="PK683_13790"
     CDS             complement(1528..>1735)
                     /locus_tag="PK683_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HNH09566.1"
                     /translation="SALGVGLFVEKTRRLSAWGIIALLIAVFPANIYAYYADINLGAP
                     LWVNLLRLPVQGLFIWWAYSFTKD"
BASE COUNT          513 a          358 c          419 g          445 t
ORIGIN      
        1 gtgcccattg gaaaccgctt gggagaccga aacgaagaaa ctgccggatc agtccaaagt
       61 caggacgcca ggaacttctg attctgtaaa gtctctcatt ttcctgactg aaaatcaaag
      121 ccaggccgaa ggcggctgcc cccaatgtgg cgatggcagt ggcataaccg gccccggctg
      181 ttcccatttt aggaaaacct aaattgccaa aaatgagtgc ataatctaaa attacattca
      241 gtatcaggcc tgtgagattg atcaaagtga cagttctcgt ctttcccagt ccggtaaaaa
      301 aaccgctgac tgccgcgaca agggcagtcg ggagcgcaga gaaggcgagc gagttatagt
      361 actccacttc cagtgcctgt actgaaggag agtggtctgc cagagagaag aaccaggagg
      421 aagcaaaatt caaagtgagg aaaagcagtc ctccgatgat gctgacatga atcgactgcc
      481 atacactgct gccgattttt tcattttcct ccgcaccata gtactgagca acaaaagcag
      541 tgacataacc tgcagtctgc tgaagcagag ccattggaac ccagaacact cccattaccg
      601 ccatagcggc tcccaatgct tctgtggaga acataccaag aaagattctg tcaatagtga
      661 tctgaagatt ccagaaactg tttgctatga taagaggcca ggctatttga aggattccct
      721 tcagtctttc tttttcagtc ataatggtca ttctgtcaga ttctgtatgg gcggcgattc
      781 tgaaaaggca aatccgtact gattctcagg tgcagaattt ggatgcagcc agttttgatt
      841 cttttcttga ctttggatca gatttgtgaa tgatattact atgatatcca ttttggaaaa
      901 actgattccc tggtatgaaa atctgaaagt gaccagggaa gagtaccttg atttggagga
      961 agacggtttt ttgtatgata tgattgaagg agttctgcat ttgtccccca gtctcgactt
     1021 taagcattct gatattgccg gaaattttta ttttgaggtc agaaagtatc tccagaatca
     1081 ttctgtaggt aaagcagtgt ttgagactga tgtgcttctc cccgaataat atgctcagga
     1141 cgactgaatg aaaggatctg taataatcgg aatgagcatt cttggaatct gcggatgtct
     1201 gtttatctcg gatagatttc agggatatgg agaggatgcc gaatatgcga aacttcagga
     1261 aagctggagt gaataccagg gtgaaatgaa ctggtcggat gcagatacaa agtgtaaagg
     1321 tctgggaatg aaactggctt caagagagga atttaatgct gcaagggcag gggatgcaat
     1381 cggaaaaatt cgtaagcacg ggaatgcttt ctggacatca gaacttttcg gaactgagcg
     1441 tgcccatgct tacggaacag caaaaggatc cgatttcatt cagttcaaaa ctgaaaaaaa
     1501 tcacgttgtc tgcattaaga ataaacctta atcctttgta aaactgtatg cccaccatat
     1561 aaaaagtccc tgcactggca gtctcaaaag atttacccag agaggggcac ccagatttat
     1621 gtccgcatag taggcgtata tgtttgcagg aaatactgcg atcagaagtg cgattattcc
     1681 ccaggcggaa agacgtctag tcttttcgac gaacagaccg acaccaagtg ccgat
//
LOCUS       DAOQGJ010000936         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49723_length_1735_cov_2.299405, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000936 DAOQGJ010000000
VERSION     DAOQGJ010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49723_length_1735_cov_2.299405"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1735
                     /locus_tag="PK683_13795"
     CDS             <1..>1735
                     /locus_tag="PK683_13795"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09567.1"
                     /translation="GVGKNALVYHINSLRNLPTIRFSFNEDTLPEDLIGSYRIHPSGK
                     GFEWKNGPLTSALQTGSAFVADEMNLASPSVIKRFSTVYESFYLDLLEGGGEKISSVD
                     GFHFIATQNPSEGFEGRKNLSYEITKNFSVVYIDSYPPEEIYQILKNLYPELLESVIK
                     NIINVNFETENRVLRGELGRNDLEKYHFNLRTMKRLSARLLNYNCNDTDILYQHFREI
                     YVDCFRNAEDRAKQTELVSSVLSYKSDRKISRVEIFSDAEYIYCNDKRIRIKNPERTK
                     EILSAVPMTASLRGFLEKVFTCHEMGENILIEYSEEDDPSIVMNLLADFSGMKTEFIH
                     LCKGIHTSDIIGALKPVSDAGQENTGRVEWVDGPLSRGIEEGSHIMITSLETAGAELV
                     EKFNMLTDDARALLLPAESGKKEELHLSPEARVYAFKVFRKTKSVSTISRAFRNRFTS
                     VLFPQLNNEECVYDILKFYLPESSLLNLMREFHMKIRTMAEKRTIGSANLNPYIFGIP
                     NLLSWIRHILHFNSENLEETVFRGAGVAYINQIYDAKERNELEKLLKKGWDLGKIPQG
                     LFHEIESKKKTF"
BASE COUNT          533 a          352 c          384 g          466 t
ORIGIN      
        1 aggagtagga aaaaatgcac tggtatacca tatcaattct ctccggaacc ttcccactat
       61 ccggttcagt ttcaacgaag acactcttcc tgaggatctg atcgggtctt acaggattca
      121 tccgtctgga aaagggttcg aatggaagaa tggtcccctg acctcagctc ttcagacagg
      181 ttctgcattt gttgcggatg aaatgaacct ggcttctccg agcgtcatca aaaggttttc
      241 gactgtatat gaatctttct atctggatct tttggaggga gggggagaga aaatcagttc
      301 agtggacggg tttcatttta ttgccactca gaatccctcc gagggatttg aaggacgaaa
      361 aaacctttcc tatgaaatta ccaagaactt ttcagttgta tatatcgatt cctatccccc
      421 ggaagaaatc taccagatac tgaaaaatct ttatcctgaa cttctggaat ccgtcataaa
      481 aaacatcatc aatgtgaatt ttgaaactga aaacagggtc cttcggggcg agctcggaag
      541 aaacgatctg gaaaaatatc atttcaatct cagaacaatg aaaaggcttt ccgcaaggct
      601 tctgaattac aactgcaatg acactgatat actttaccag catttcaggg agatctatgt
      661 ggactgcttc agaaatgcag aagacagggc aaagcagact gaactggtat cttctgtcct
      721 ctcttacaag tccgacagga aaatctcccg agtcgaaatt ttttcagatg cagaatatat
      781 atactgtaat gataaaagaa tacgcattaa gaatcctgag aggacaaagg aaattctgtc
      841 tgcagtgcct atgactgcgt cacttagagg atttctggaa aaagttttta cctgtcacga
      901 gatgggagag aacattctga ttgaatattc tgaagaggat gatccttcca tcgtaatgaa
      961 tcttctcgcc gacttttcgg gaatgaaaac agagttcatc catctctgca agggaatcca
     1021 tacctcggac atcatcggtg cactgaagcc ggtttcggat gcgggtcagg aaaacacagg
     1081 cagggtggaa tgggtggacg gtcctctttc cagggggatt gaagagggat ctcatataat
     1141 gataacttca cttgagactg ccggtgcgga gcttgtggaa aaattcaata tgctcactga
     1201 tgatgcgaga gcccttcttc ttccggcaga aagcggaaag aaggaggaac ttcatctttc
     1261 tccagaggcc agagtctatg cattcaaggt gttcagaaag actaagtctg tttccacaat
     1321 ttccagggca ttcagaaaca ggttcacatc agtccttttc cctcagctga acaatgaaga
     1381 atgtgtatac gatatcctga aattttatct tcctgaatct tcccttctga atctgatgag
     1441 ggaatttcat atgaagatca gaactatggc agagaagaga acaatcggtt ctgccaatct
     1501 gaatccgtat atattcggta tcccgaatct tctttcctgg atacgtcata tccttcactt
     1561 caattccgaa aatctggaag agacagtctt cagaggagca ggagttgcct acatcaatca
     1621 gatttatgat gcaaaggaaa gaaatgaact ggaaaaactt ctgaaaaaag gctgggatct
     1681 gggaaaaata cctcagggac tttttcatga aatagagagt aaaaaaaaaa ctttt
//
LOCUS       DAOQGJ010000937         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49756_length_1734_cov_3.320429, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000937 DAOQGJ010000000
VERSION     DAOQGJ010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49756_length_1734_cov_3.320429"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK683_13800"
     CDS             <1..482
                     /locus_tag="PK683_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNH09568.1"
                     /translation="AVQFRRSNLEETVKRALKDSGLNPELLELELTESILIHDTENVL
                     NTIKRLKKIGVKLSIDDFGTGYSSLSYLKRFAVDKLKIDQSFVRELHQNQEDAAIVRA
                     VVQMAKSLNLRTIAEGIESENILQILAEYGCNEVQGYYFSKPVPHHLFEDFFWQCRQ"
     gene            587..1645
                     /locus_tag="PK683_13805"
     CDS             587..1645
                     /locus_tag="PK683_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH09569.1"
                     /translation="MKPAVNSFFSIQTVHHWKLSLLLFLLSLSPSLSAENLIQDELEK
                     AILRENLDAVKFFIRQGASSGRPNQKGAYPLHIAAKTGSFEIAKFLSEHSGKPDPKEE
                     KFKVTPLHISSYLGNETLTKYLIEKKAELNHRDELKGFTALHFAAENSRLNTIKALLE
                     AGAEKNIKDKNGKTFIEHLNSTDKDYMENYLQVRSEEIRNSGCRELLDGINELGLKYN
                     ETNHITQGEIFEKLYLSSDESKREYCIMKYILPDPDKFYWISIFLFDEEYTKIKNFSL
                     AEKLLGRAENFCKEESCVPIYFIHALIYWRQNKKSESKKFFEKAIRLSEKYPGNIPAI
                     KEDERKEIHKFLGLTEKD"
BASE COUNT          579 a          350 c          354 g          451 t
ORIGIN      
        1 cagccgttca gttcaggaga agcaatctgg aagaaacagt caaaagagct cttaaggatt
       61 caggcctcaa tccggaactt cttgaactgg agctgaccga gtctattctg atacacgata
      121 cggaaaatgt gctgaataca attaaaagac tgaagaagat tggagtcaaa ctttctattg
      181 acgatttcgg cacaggttat tcgagccttt cctatctgaa aaggtttgca gtagacaaac
      241 tgaagataga tcagtctttt gtccgtgaat tacatcagaa tcaggaagat gcagctattg
      301 tcagagcagt agtccagatg gccaaaagtc tgaatctgag aacaatcgct gaaggaatag
      361 aaagcgagaa catactccag attctcgctg aatacggctg caatgaagtg caggggtatt
      421 atttttccaa accggttccc catcatcttt ttgaagattt cttctggcag tgcaggcagt
      481 aaactgtcat ttgattcgag agccgtttca ctgaaactga atcctttttc atccttttcc
      541 attttatttg tttccaattc agaactctgt ttaaaatcgg tgaacaatga aacctgcagt
      601 aaattcattt ttttctattc aaaccgtcca ccactggaaa ctgagcctgc tcctcttcct
      661 tctcagtctt tctccatccc tgtctgccga gaatctgatt caggatgaac tcgaaaaagc
      721 aattcttcga gaaaatctgg atgcagtgaa gttttttatc agacaggggg catcgagcgg
      781 cagaccgaat cagaaaggag cctatccact gcatattgcg gcaaagacag gaagcttcga
      841 aatcgccaaa ttcctgtcag aacattcagg aaaaccggac cctaaggaag agaaattcaa
      901 ggtaactcct cttcatattt cttcctatct ggggaatgaa acactgacga aatacctgat
      961 tgaaaaaaaa gcagaactga atcacagaga tgaactgaaa ggattcactg cacttcattt
     1021 cgcggcggag aattcccggc tgaacacaat caaggcactt ctggaggcag gtgcggaaaa
     1081 aaacataaag gacaagaacg gaaaaacttt catcgaacac ctgaacagta cagacaaaga
     1141 ttatatggaa aattatcttc aggtaaggtc ggaagaaatc agaaattccg ggtgcagaga
     1201 acttctggac ggaataaatg aactgggact gaaatacaat gaaaccaatc atatcactca
     1261 gggagaaatc tttgagaagc tttacctttc ttctgacgaa tccaagagag aatactgtat
     1321 aatgaaatat atccttcctg atcctgataa attctactgg atttccatat ttctgtttga
     1381 tgaggaatat acaaaaatta aaaatttctc cctggcggaa aaacttctgg gaagagcaga
     1441 gaatttctgc aaggaagagt catgtgtacc tatctatttt atccatgcac tgatttactg
     1501 gaggcagaat aaaaaatcgg aatccaaaaa attctttgaa aaggcaatcc gcctttctga
     1561 aaagtatcct ggaaacattc ctgcaatcaa ggaagatgag agaaaagaaa tccataaatt
     1621 ccttggactg accgaaaagg actgaaataa aatgctgttc aaatagaaag acaatccaag
     1681 cagtcttgat ctctgcaaag aggcggtcgg ctacttccat caccttttct tttt
//
LOCUS       DAOQGJ010000938         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49759_length_1734_cov_3.134604, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000938 DAOQGJ010000000
VERSION     DAOQGJ010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49759_length_1734_cov_3.134604"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PK683_13810"
     CDS             <1..704
                     /locus_tag="PK683_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09570.1"
                     /translation="PKTAYKFLTKNKIIKSDRKSTIEAMLEWCRSNLSHSSGDIGDVD
                     SYYDQWQYKGLPPVVKMIEGTKTLSDPSAEKRHYTAGCGGTSAFLKAVLRVVNIPVTT
                     VTVGAGHKTPYFMEEGLYLSHGDDPYNLLMINDPPIPISELFLDEKKFEEWFGEDVSD
                     DQSAANVGRVPVELSLKYLPKYLIQDYKEDIEEGKSHSEGAVFENYSYNYTVEELEEM
                     GLWKRLDKKLKELED"
     gene            complement(818..1504)
                     /locus_tag="PK683_13815"
     CDS             complement(818..1504)
                     /locus_tag="PK683_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09571.1"
                     /translation="MWTDSSGMQDMPVPREMNAVDLENTKNEFVSASKNAVEAGFDGV
                     ELHSANGYLLEQFLNPGSNQRKDSYGGSIENRIRYVLETAEAVAAAIGKNRVGIRLSP
                     YSTFNDMSSFAETEEQYVLLAEKLNALGIAYIHLADNTGGKAKSTFGKIRKAFKNTLI
                     LSVGYTKEKAEEDLASGAGDLIAFGKPFLSNPDLLTRFEKGKPLNDFDASTFYAADAK
                     GYTDYPFLKD"
BASE COUNT          497 a          374 c          397 g          466 t
ORIGIN      
        1 aaccaaaaac tgcctataaa tttttgacta aaaacaagat tatcaagtcg gacagaaaaa
       61 gcacaattga agcaatgctt gaatggtgca gatcaaatct ctcgcacagc agcggagaca
      121 ttggcgatgt tgattcctat tatgatcaat ggcagtataa aggacttccg cctgttgtca
      181 aaatgattga aggaaccaag accttaagtg acccatcagc agaaaaacgc cattatactg
      241 cgggatgcgg gggcacatcg gcatttctga aagcagtact ccgggttgta aatattcctg
      301 ttaccactgt cacagtcgga gcaggtcaca aaactccgta ttttatggaa gagggacttt
      361 atttaagcca cggagatgat ccatacaatc tgcttatgat caatgatcct cccattccga
      421 tttctgaact ctttttagac gagaaaaagt ttgaagaatg gtttggagaa gatgtatctg
      481 acgatcagtc tgctgcaaat gtcggacggg ttcctgtgga attatcatta aaatatcttc
      541 caaaatatct tatccaggac tataaggaag acattgaaga aggaaagagc cacagtgaag
      601 gtgcagtttt tgaaaactac agctacaatt atacagttga ggaactcgaa gaaatgggac
      661 tctggaaaag gcttgataaa aaactgaaag aattggaaga ctgagcttta ggattttgca
      721 aaagctcagt ctttaagctt ccgatatctt ggtcctaggc tcacatataa tctgtgagcc
      781 ggatgattta tcgaagtctg aaaaaatttt cagaaattca gtctttcaga aaaggatagt
      841 ctgtataacc ttttgcatct gctgcataga atgtggatgc atcaaagtca ttcagaggtt
      901 ttcctttttc gaatctagtg agcagatcag gattggaaag gaacggtttt ccgaatgcga
      961 tcagatcgcc tgctccggat gcaaggtctt cttctgcttt ttcctttgta tatcctacag
     1021 aaaggatcag agtattcttg aaagccttac gaatttttcc gaatgtggat tttgccttgc
     1081 ctccggtgtt gtccgccaga tgaatataag caattcctaa tgcattcagt ttctccgcca
     1141 gaagaacata ctgttcctcc gtttctgcaa aggaagacat atcattgaat gtactgtaag
     1201 gagaaagacg gataccgacg cggttctttc ctattgcggc tgccacggcc tctgcagttt
     1261 ccagcacata tcttattctg ttttcaatgc ttccgccgta actgtctttt ctctgatttg
     1321 atcccggatt cagaaactgt tcaagaagat aaccgtttgc agaatggagt tccacaccgt
     1381 caaaacctgc ttccactgca tttttagaag cggatacaaa ttcatttttt gtattctcca
     1441 gatcgactgc attcatttct ctaggaacag gcatatcctg cattcctgag gaatctgtcc
     1501 acatctgtcc ttttgctgcg actgcagacg gagcagcaac agaaagacaa cgcccagatg
     1561 ggcgccgcta tgagcatggc aggcagcgcc atcggcgcgg gcatgggcat gattcctaag
     1621 ggatgagcgg gcgcggagga cgagcatggt cgactggaac aagtaccgtg acgagctcat
     1681 caaggccggt gtcgtcgccg acccgcgaag caagctcgcc cagaccggcc ggcg
//
LOCUS       DAOQGJ010000939         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49818_length_1733_cov_2.916567, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000939 DAOQGJ010000000
VERSION     DAOQGJ010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49818_length_1733_cov_2.916567"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..>1733)
                     /locus_tag="PK683_13820"
     CDS             complement(217..>1733)
                     /locus_tag="PK683_13820"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH09572.1"
                     /translation="LEFSPNIVNYVIRTKSIVLLNDASRSGMFINDAYVKDKLPKSIL
                     CYPIVNQGNLAGLVYLENNLTANAFTPERMEILKILASQIAVSVENSVLYANLEEKVE
                     ERTRDLNRALTEVSALKVQQDGDYFLNTLLIEPLGQNNAVSQTVKVEFFLKQKKEFTF
                     RKDVYELGGDINISDNIKLNSIPYIVFLNGDAMGKSIQGAGGVLVLGTVFKSILQRTN
                     DTESLRSMYPERWIRNAFVEMHKVFESFEGTMLMSLVFGLIDEMTGMMYYMNAEHPDP
                     VLYRDEKAEFLPNPVGIFRKLGTQGQPDSRLQVSVFRLRPQDKVILGSDGRDDFVISR
                     DELTGQFIINQDENLFLESVEAGEGHLEKIYEDVMSRGQQFDDFSLLSIVYNGAEVQE
                     SYEKSISKVKELISAKDFAAASSEGELLMGKFPEYAEVFLISAQVYLELKKYSSAADL
                     AERVRLRDSRNLQAYEILLDSYSALGRNDRVRELLVTANKFFPNLEKFQKFAEL"
BASE COUNT          468 a          449 c          309 g          507 t
ORIGIN      
        1 tcagcttctg atcagtctga ctgacgggaa agtgactata gaagaacatt catcggcata
       61 gcagagtatg aaaatccatg acctgcgtaa aactgcttcc ggcatcggta cttttccatt
      121 cggtatatcc tttggcaatt gcatacactg catctggatt tgtaagagaa tattccatat
      181 caaggaactg aggcttgggg tccgaaaaga gttcattcag agttccgcaa atttctgaaa
      241 tttctccaag ttaggaaaga atttattggc tgtcaccaaa agttccctta cacggtcgtt
      301 tcttcccaat gcactgtacg aatccagaag tatctcatat gcctgaagat tcctgctgtc
      361 cctcagtctg accctctctg caagatccgc agctgaactg tattttttca gttccagata
      421 tacctgtgcg gatatcagga acacttccgc atactcagga aattttccca tcagcaattc
      481 tccttcagat gatgctgcag caaaatcttt cgcactgatc agttccttta ccttggaaat
      541 gcttttctca taagattcct gaacctctgc tccattgtac actatggaaa gcagtgagaa
      601 atcgtcaaac tgctgaccgc gagacatcac atcttcatat attttctcca gatgaccctc
      661 gcctgcctcc actgattcca aaaaaagatt ctcatcctga ttgattataa actgaccagt
      721 cagttcatct ctggaaatca caaaatcatc tcttccgtca gaacctaaga tcaccttgtc
      781 ctgtggacgg agtctgaata ctgatacctg aagtctgctg tccggctgtc cctgtgtccc
      841 aagcttgcgg aaaataccca ccggattcgg aagaaactct gccttctcat ccctgtacaa
      901 aacaggatca ggatgctcgg cgttcatata atacatcatt cctgtcatct cgtctataag
      961 tccgaataca agagacatca gcatcgttcc ttcaaagctc tcaaagacct tatgcatctc
     1021 cacaaacgca ttcctgatcc agcgttccgg atacatgctt ctcaggcttt ccgtatcatt
     1081 cgttctctgc agtatcgact tgaatacagt tccaagaaca agaactcccc ccgctccctg
     1141 aatcgacttc cccatcgcat ccccgttcag gaaaactata tagggaatac tgttcaactt
     1201 tatattatca gatatattaa tatctccgcc aagctcatac acatccttgc gaaatgtaaa
     1261 ctccttcttc tgcttcagaa agaattccac tttcactgtc tggctcactg cattgttctg
     1321 cccaagaggc tctatcagaa gggtgttcag gaaatagtcg ccgtcctgct gcaccttcag
     1381 tgcactcacc tctgtcaggg ctctgttaag atccctggtt ctctcttcca ctttctcttc
     1441 caggtttgca tacagaacgg agttttccac cgaaactgca atctgcgatg caagtatttt
     1501 cagaatctcc atcctctcag gagtgaacgc attcgctgtc agattatttt ccagataaac
     1561 aagtcctgca agatttccct ggtttacgat tggatagcaa agtatggatt ttggaagctt
     1621 atcctttaca tatgcatcat tgataaacat acctgatctt gaggcatcgt taaggagaac
     1681 aatacttttc gttctaatca cataattcac aatgttcgga gaaaattcca gag
//
LOCUS       DAOQGJ010000940         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49868_length_1732_cov_3.305307, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000940 DAOQGJ010000000
VERSION     DAOQGJ010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49868_length_1732_cov_3.305307"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1732
                     /locus_tag="PK683_13825"
     CDS             <1..>1732
                     /locus_tag="PK683_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09573.1"
                     /translation="AFLPSLGQGYGAAGLFFASLIPVFFLSADLEKSEGKEGKFFLLL
                     GLYGTFSVFTVYTAGPSNGQTAVFPMQKGFILLWILVFCEIIAFRFGNIGKKSDGMNL
                     FFIFEILMLSAVLVVASMSSFEILKTSVFRSNIPNERFILLALPAVSAMIFLHSVRRH
                     ISSLHIFMILSVSTCFILGNELLTALSGNVRFQSRDWIFAFSVIVPVSGLFLAERFEE
                     KIERKLQAWGMISGSVIALYLTVWTNQMTSVWTILPAGIFGFYFLAGAHFTGGFKRRA
                     SHLAAALGTVCFVSFIISYLNLNNFQSHLGIIFGVTSILYSLSGFYFDSRSEDKEDLS
                     LFRPLDDNSLITAFLSAVFLSNVTGSTGILENSLVLFTGLNLIFRSFRDKSVLISFLG
                     VFILSLSVMRHLFSYFTQQNTADTALIAALTALLLQTGAVIFPNAGKPTPKARKMLTA
                     VRLPFPADGLSLLHFGLSFTSFLYIIFSVFQTVIWFGLSGQPDRNLVIIANTVIALIF
                     FISFTTHSFSVFSLKGSTAALGLIFISVGLAAVANRIGRPLPPPVVGTNLTLGIGALW
                     LFSRLLFHKGP"
BASE COUNT          415 a          410 c          342 g          565 t
ORIGIN      
        1 ggcattcctt ccctctctgg gacagggata cggagcagca ggattattct ttgcttcact
       61 gattcctgta ttttttctct cggccgatct ggaaaaatcc gagggaaaag aaggaaaatt
      121 ttttcttctc ctcggacttt acggaacatt cagcgtattt acagtatata ctgcaggtcc
      181 ttcaaatgga cagactgctg tctttccaat gcagaaagga ttcattcttc tctggattct
      241 tgtattctgc gaaatcattg ctttccgatt cgggaatatc gggaaaaaat cagatggaat
      301 gaatctgttc ttcatttttg aaattctgat gctttcagct gttcttgtag ttgcatctat
      361 gtcttcattt gaaattctga aaacttctgt tttcagatca aatatcccga atgaacggtt
      421 tattctgttg gcgctccccg ccgtatctgc aatgatattt ctgcattcag tcagaagaca
      481 tatttcctca ctgcacatat ttatgattct ttccgtaagc acctgtttta ttctgggaaa
      541 tgaactgctt actgccttgt ccggcaacgt aagatttcag agcagggact ggatatttgc
      601 tttttctgtg atcgtaccag tttcagggct ttttcttgcc gaaagattcg aagaaaaaat
      661 agaaaggaaa cttcaggcct ggggaatgat ttcaggatca gtgatcgccc tttaccttac
      721 tgtatggaca aatcaaatga catcggtctg gacgatcctc cctgctggca tcttcggttt
      781 ttattttctg gcgggagcac attttacggg agggttcaag agaagggcaa gccacctggc
      841 tgcagccctc gggacagtct gctttgtttc tttcattata tcatatttaa atctgaacaa
      901 ttttcagtct catctcggaa ttatattcgg cgtgacaagc atactttaca gtctttccgg
      961 attttacttt gattccagat cagaggacaa agaggatctt tctcttttca ggccgctgga
     1021 tgacaattcc ctgattacag ccttcctctc tgctgtcttt ctaagcaatg tcaccggcag
     1081 tacaggaatt ctggaaaact cacttgttct cttcaccgga ctgaatctga tcttcagatc
     1141 gttcagagac aaatctgtcc tcatcagttt cctcggagtt ttcatacttt ctctttcagt
     1201 aatgagacat ctgttcagtt atttcactca acagaatact gcagataccg cactgattgc
     1261 tgcattgaca gcacttcttc tgcagacagg tgcagtcatt tttccgaatg caggaaaacc
     1321 gactccgaag gcaagaaaaa tgcttacagc agtccgtctt cccttccctg cagacggact
     1381 gtctcttctg cactttggac tatcatttac atcctttcta tacatcattt tcagcgtgtt
     1441 ccagactgtc atctggttcg gtctttccgg tcagccggac agaaatcttg taatcatcgc
     1501 aaatacagta attgcactta tattcttcat ctcttttacc actcattcct tttcagtctt
     1561 ttcactcaaa ggaagcactg ctgcactggg actcattttt atcagcgtcg ggcttgcagc
     1621 tgtcgcgaac agaatcggca gacccctgcc gccccctgtt gtaggaacaa atctgactct
     1681 tggaatcggt gccctctggc ttttctccag gcttcttttt cacaaaggac ca
//
LOCUS       DAOQGJ010000941         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49884_length_1732_cov_2.663089, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000941 DAOQGJ010000000
VERSION     DAOQGJ010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49884_length_1732_cov_2.663089"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1732)
                     /locus_tag="PK683_13830"
     CDS             complement(<1..>1732)
                     /locus_tag="PK683_13830"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH09574.1"
                     /translation="DVHCIALTSYQGGHVEFFSYIKDLLKKFGSPHIRIFGGGGGTIL
                     PSEADYLHTYGIDRIYSPDDGRSLGLQGMINDLLIKSDCSVIEKKLQGKDSDSMTGRV
                     LSFIELDREIPSEIIEPLQIHGKRTAPVIGITGTGGAGKSSFTDEMIRNLTENNPGLK
                     IAVLSVDPTRKKTGGALLGDRIRFNSARNRNVFLRSIATHSENSSISPSVKSISRFLK
                     NSIYDLVFLETAGTGQSDSQVTDVSDFSIYVMTPEYGADTQLEKIGMLDYADMVVINK
                     SDRKGAADARKSVAKQIQRNRGLFSESPESMPVFSTVASQFNHPGIIEACRFLSRKIS
                     GMFSLPLDTFNDKIMISENKSIIPPSRVRYLSEIRENSEEYCRETEIQSEAAEEIYSL
                     EKIISRTEEGELKTELNRRLSELQSKLSAESKKIISEWETRKKNYESQFYSYTVRGKE
                     IRVPNFSSSLSGLRIPKVCLPEYRNYGDILKWAYSENVPGEFPYTAGVYPFKRTNEDP
                     IRMFAGEGSPERTNRRFHYLSKGQPAVRLSTAFDSVTLYGEDPEERPDIYGKIGNSGV
                     SICSLDDMKRL"
BASE COUNT          410 a          400 c          374 g          548 t
ORIGIN      
        1 agagcctttt catatcgtcc agggagcaga tggacactcc cgaatttcct atttttccgt
       61 agatgtccgg tctttcttcg ggatcttctc cgtaaagagt caccgagtca aatgcagtag
      121 aaagtctgac tgcaggctgt cccttagaca gataatgaaa ccttctgttt gtcctttccg
      181 gcgaaccctc tccggcaaac atcctgatcg gatcctcatt tgttcttttg aaggggtaga
      241 ctcctgccgt gtatggaaat tcgccgggta cattttccga ataagcccat tttaatatgt
      301 ccccatagtt tctgtactca ggcagacaga ccttcggtat tctgagaccg gacagagagg
      361 agctgaaatt gggaacccgt atctcttttc ctctgactgt gtaggaatag aactgtgatt
      421 cataattttt tttcctggtt tcccattcag aaataatttt ttttgattca gcagaaagtt
      481 ttgactgaag ttcggaaagc cttctgttca gttctgtttt cagctctcct tcctctgtcc
      541 tggaaattat tttttcaagg gaatatattt cctctgccgc ttcagactga atttccgtct
      601 ccctgcagta ttcttcagag ttctccctga tttccgaaag ataacgcacc ctggaaggag
      661 gaataatgct tttattttca gaaatcatta ttttatcatt aaatgtgtca agcgggagag
      721 agaacatccc gctgatcttt ctggaaagga atctgcatgc ttcaatgatt ccgggatggt
      781 tgaactgaga ggcgactgtg ctgaataccg gcatggattc aggactttca gaaaacagtc
      841 ccctgtttct ctgtatctgc ttcgccacac tttttctggc atccgcagca ccctttctgt
      901 cggatttgtt gatgacgacc atatcggcat agtcgagcat tcctattttt tcgagctgag
      961 tgtccgcgcc gtactcagga gtcattacat aaatcgagaa atcggataca tcagtaacct
     1021 gcgagtcgga ctgtcctgtt cctgcagtct caagaaatac aagatcgtat atgctgtttt
     1081 tcagaaacct ggatatgctt ttgactgaag ggctgatgga ggagttttca ctgtgggtgg
     1141 caatggagcg aagaaacaca tttctgtttc tggcagaatt gaatctgatc ctgtctccta
     1201 aaagtgctcc ccctgttttt ttcctggttg ggtctactga aagaacagca atcttcagac
     1261 cgggattgtt ctccgtcaga tttctgatca tttcgtccgt gaaggaagat tttcctgctc
     1321 ctcccgttcc tgtaattcct atcacgggag ctgtcctctt tccatgaatc tggagaggtt
     1381 caatgatttc tgatggaatt tccctgtcca gttcaatgaa gctcagcact ctgcctgtca
     1441 ttgaatctga atcctttccc tgcagttttt tttcaatcac tgagcagtcg ctttttatga
     1501 gcagatcgtt gatcattccc tgcagtccaa ggcttcttcc gtcatcgggt gaatagatcc
     1561 tgtcaattcc ataagtgtgc agatagtcag cttcagaggg aagaattgtt cctcctcctc
     1621 ctccgaaaat acggatatgc ggagagccga attttttcag aagatccttt atgtagctga
     1681 aaaactcgac atggcctccc tgataggaag taagagcaat gcagtgaaca tc
//
LOCUS       DAOQGJ010000942         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49917_length_1731_cov_3.394391, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000942 DAOQGJ010000000
VERSION     DAOQGJ010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49917_length_1731_cov_3.394391"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1092)
                     /locus_tag="PK683_13835"
     CDS             complement(226..1092)
                     /locus_tag="PK683_13835"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNH09575.1"
                     /translation="MGFSGLFEILEFRPMMKSMQVIIFMLLYFPFISVYSDSFIQTSP
                     ALQETDIAKYAEISEQEFSDCRKAVESSEGLWQKNIKESIVYPGEKKSLWLRWNLENQ
                     DAQSIEKYILFFWNNPEKIRICQNQEESWSLVQAEMQLKDQHWIHELILPVIKISFSP
                     NEKKSVYIRIESHQSVKIPVSLVEQEELIAQIKLTSNLLWLFGGFVLIIILYNIFQIF
                     YAGDSVNYYYILYILSMAASVFLSFGQGNRIFAVLKDFQNIEFLFLGLSGFGAAQFSR
                     KFLFFQILYFRG"
     gene            1174..>1731
                     /locus_tag="PK683_13840"
     CDS             1174..>1731
                     /locus_tag="PK683_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002970824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNH09576.1"
                     /translation="MDFKSIFENFLNPPVMFFFLGLLAMFLKSNLTIPDQLSKFFSMY
                     LLFSIGFKGGHELYSTQFSMEHVWTLFACTAMAVVVPLYSYFLFRTKLDVHNSAALAG
                     SFGSVSAVTFVTAGSFLHNKGLEYGGFIVAGLALMESPAIIIAVILDRVSRRKDSVHK
                     ETFSWRELIHEAFLGDSVFLLMGALV"
BASE COUNT          506 a          356 c          355 g          514 t
ORIGIN      
        1 ggggagaaca gcgagagaac gagttttcat tgtactgtgg atgatgttga ttatgattac
       61 aatcattatt attactacca ttatcatcat cgccattttc aagagagtaa gcgcaatagt
      121 cagccggacg ctaatggtga tggagaaggt gttgcggtct cttagttagg ctgaagtttg
      181 gaacagtttc gaaaaaactt ttggcgggaa ctctcttgta taaatttatc ctcggaagta
      241 caaaatctga aaaaaaagga attttctgga aaactgagcc gcaccgaaac cggaaagacc
      301 cagaaaaaga aattcaatat tctgaaaatc cttcaagaca gcaaagattc tgtttccctg
      361 tccgaagctc aggaaaacac ttgcggccat cgacaaaata taaagtatat agtaatagtt
      421 gactgaatct cctgcataaa agatctgaaa gatattatat agaataatga taagcacaaa
      481 acctccgaaa agccagagaa ggtttgaggt cagcttgatc tgcgcaatca gttcctcctg
      541 ctccaccaga ctgaccggaa tttttacgct ttgatgagat tcgatcctga tgtagacact
      601 tttcttttca ttgggagaga aactgatttt tattacaggg agaatcagct catgaatcca
      661 gtgctgatcc ttcagctgca tttccgcctg aacgagactc cagctctctt cctgattctg
      721 gcagattcgg attttttcag gattgttcca gaaaaagagg atatactttt caatggactg
      781 agcgtcctga ttttccagat tccagcgaag ccacagagat tttttttctc cgggatacac
      841 gatggattct tttatatttt tctgccagag cccttccgat gattcaaccg ctttcctgca
      901 gtcggaaaat tcctgctcag atatttccgc atattttgca atatccgttt cctgcagggc
      961 aggcgaggtc tgaataaaac tgtccgaata gactgaaatg aaaggaaaat acaaaagcat
     1021 aaaaattatc acttgcatag atttcatcat tggtctgaat tccagaattt caaaaagtcc
     1081 tgaaaatccc accaaaaaaa cgttccggtt tgtaagacag gacgatttcg gttcaggtct
     1141 gtgcaagaat agaaccaagt tcaggaggcc aaaatggact ttaaatcaat attcgaaaat
     1201 tttctgaatc cgcctgtaat gtttttcttt ttaggactgc ttgccatgtt cttaaagtca
     1261 aatcttacaa ttccggatca gctttcaaaa ttcttctcaa tgtatctgct tttttccatc
     1321 gggtttaagg gcggtcacga actgtacagc actcaatttt cgatggaaca cgtctggact
     1381 ctatttgcct gcacagcaat ggcagtcgtc gttcctctct attcctattt tcttttcaga
     1441 acaaagctgg atgttcataa ctctgccgcc cttgcaggat cattcggatc tgtctcagct
     1501 gtcacattcg taactgcagg gtcatttctt cataacaaag gattggaata cggaggattt
     1561 attgttgcag gacttgcact gatggaatct cctgccatca tcattgctgt catccttgac
     1621 agagtcagca gaagaaagga ttccgtgcat aaggaaacat tttcctggag agaactgatt
     1681 catgaagcat ttttgggaga ttcagtattc cttctgatgg gcgcactggt c
//
LOCUS       DAOQGJ010000943         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49931_length_1731_cov_2.729714, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000943 DAOQGJ010000000
VERSION     DAOQGJ010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49931_length_1731_cov_2.729714"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1132)
                     /gene="mtnP"
                     /locus_tag="PK683_13845"
     CDS             complement(266..1132)
                     /gene="mtnP"
                     /locus_tag="PK683_13845"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNH09577.1"
                     /translation="MTEQVKIGVIGGTGLYDLDGMEKIDEVFPETPWGKPSDRITIGK
                     FKGKLIAFLPRHGKGHFISPSEIPNLANIAALKNLGVEEIVAFSSVGSLREEIAPLDF
                     AIPDQIIDRTKHRESTFYGNGLVAHAPFGDPFSQSLADRIKKAADRIQLKMHTGKTLV
                     CMEGPLFSTRAESKMYRQFGGDLINMSVLPESKLAREAEIAYQMVCMSTDYDSWKEHE
                     EAVTVEMVIANLNKNGANAKRLLAELVPDLGNGDDLSLKGSAKFAIITSPEKRNPEQI
                     KKFQYLFGKDFK"
     gene            complement(1129..>1731)
                     /locus_tag="PK683_13850"
     CDS             complement(1129..>1731)
                     /locus_tag="PK683_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNH09578.1"
                     /translation="PVISGRISSEFSFYSIGNTTQEFIKNFISSGSVKVESGKLLGFA
                     NLYKPIMQIGKLLNFTGPKGESTEFKSINMKFRVGQENVDIKDFKLTGVGIDAAGKGN
                     VDFKLNSDFRFNIGLAGLAGKAFSVPIVYKGTLPNVKGYIDPVWLGTVYIGSTVIPGP
                     IGTTAGGLAGNAISDYVHRAVEGLKEVFSIGIGSKKENEK"
BASE COUNT          451 a          405 c          315 g          560 t
ORIGIN      
        1 aatggaaatt acttcaattt atttttcgtg tccttcgtgg tgattttttg cttatacaca
       61 gccccaaatc cctaaatact gtatatttta tcataaatat acagtatttg atttttacaa
      121 cctgacaaag ttgcaataat acttgtcggc agatcataga aggtttgtca tatgagcttg
      181 tcaaaccatg gtctatcggc acccttcgac aagcaaactt ggaaactgct gctcagggtg
      241 acatagcttt aattgaactg caaccctact taaaatcctt tccgaaaaga tactgaaatt
      301 ttttgatctg ctcagggttt cttttttcgg gtgaagtgat gattgcaaat tttgcacttc
      361 ctttcaaaga aagatcatct ccgtttccga ggtcaggcac cagctctgcc agaaggcgtt
      421 ttgcatttgc accatttttg ttcagattgg caattaccat ttccacagtc acagcttcct
      481 cgtgttcttt ccaggaatca taatcagtgg acatacatac catctgatag gcaatctctg
      541 cttctctggc cagtttagat tcaggaagga cactcatatt gatcagatcg cctccgaact
      601 gcctgtacat ttttgattct gctctcgtgg aaaacagagg accttccata catacaagtg
      661 tttttcctgt gtgcattttc agttgaatcc tgtctgcagc ctttttgatt ctgtctgcca
      721 ggctttgaga gaacggatca ccgaaaggag catgagccac aagtccgttc ccataaaaag
      781 tggattccct gtgttttgtt ctgtcaatga tctgatcagg aattgcaaaa tcgagaggtg
      841 caatttcttc acgaagactt cctactgaac tgaaagctac aatctcttcc actcccagat
      901 ttttgagtgc agctatattt gcgagattcg gaatttctga aggagatata aaatgccctt
      961 tcccatgtct gggaagaaat gcaatcagtt ttcccttgaa ctttccgatt gtaattctgt
     1021 cagaaggttt accccaggga gtctccggaa atacttcatc aattttttcc attccatcca
     1081 gatcataaag tcctgttccg ccaatcacac cgattttcac ctgttcagtc acttctcatt
     1141 ctcctttttt gagccaatac cgattgagaa cacttccttt aatccttcta ctgccctgtg
     1201 cacatagtca gaaattgcat ttccggcaag gcctcctgcc gttgtgccga ttgggccggg
     1261 aatgactgtg cttccaatgt acacagttcc cagccatact ggatcaatat atccctttac
     1321 attaggaagt gttcctttgt atactatagg gacactgaaa gctttgcctg caagtcctgc
     1381 aagccctata ttgaaccgaa agtctgaatt tagcttaaaa tccacatttc ctttgcctgc
     1441 agcatcaata ccgacccctg tgagcttaaa atccttaata tctacatttt cctgcccgac
     1501 cctgaacttc atatttatgg atttgaattc agtgctctcc cctttgggtc ctgtgaaatt
     1561 caggagtttt ccgatctgca taatcggctt gtaaaggttc gcaaatccca gaagttttcc
     1621 gctctcgact tttacagaac ctgaacttat aaaattcttt atgaattcct gagtcgtgtt
     1681 tccaattgaa taaaaactga attcagagga gattcttccg ctgatgacag g
//
LOCUS       DAOQGJ010000944         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49942_length_1731_cov_2.211814, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000944 DAOQGJ010000000
VERSION     DAOQGJ010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49942_length_1731_cov_2.211814"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PK683_13855"
     CDS             complement(<1..800)
                     /locus_tag="PK683_13855"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNH09579.1"
                     /translation="MKFLILLFVFSVHSLYSESKKNIPIEILISSDNRIYEQGLYGIQ
                     SIIGTDVKVSYLDIVIAENPEISNYFKKIEDENPPFIITIGPSATKTAREHLKNTPVL
                     FSMINAPKSLGLSQGKLCGVSMDISVTEFFTTLKDINKEIRNVYSFYSTSEGEYAAGE
                     GEYSDLQYKIIYNKIKITDKKDFSKELNALAGKAEAFLMVNDPLYGKNEFEELSNFCK
                     KNKIILMTSFPALVKAGATFGISPDYSKIGVLTGQMAERILLKTGGAN"
     gene            complement(797..>1731)
                     /locus_tag="PK683_13860"
     CDS             complement(797..>1731)
                     /locus_tag="PK683_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH09580.1"
                     /translation="FNESYEALPSDNKLPPEASSDVQKSLVHLEKGELEEAYHGIEKF
                     LDDYREFPDLLKVLGKYYFGVGEFAKAIECYDQYLKLVPGDNEYLLLLSDAYRLSGKL
                     NLAADVGERLFLRHQTNPKNLLNLGRIYLQMHVFGRSENMIERLLGIEPENDEAARLK
                     IELAQAKEHRKARSNEDAPDEKAEFIYRKAETLYKAKNYLQALDQYETLMDYESYSAS
                     PKLLLKVANCNSFLGNYEKAIIYYNRSISEDPLNIHAYNNLGGVFYKLGYPEKAKLEW
                     KKALEIEEDFAPATFNLEKIDKIALNGVSGVEKI"
BASE COUNT          461 a          386 c          333 g          551 t
ORIGIN      
        1 cagttggctc cccctgtttt caacagaatt ctttctgcca tctgtccggt cagaaccccg
       61 attttactgt agtccgggct gatcccgaaa gtggcacctg ccttgaccag agcgggaaat
      121 gatgtcatca gtataatttt gtttttcttg cagaaattgg aaagttcctc aaattcattc
      181 ttaccgtaaa gaggatcatt taccatcaga aatgcttctg ctttccctgc caatgcattc
      241 agttccttgg aaaaatcctt cttgtccgta atcttgattt tattataaat aattttatat
      301 tgaagatcag agtattcccc ttctccggct gcatattctc cttctgatgt ggaatagaag
      361 gagtacacgt tccgaatctc cttgttgata tccttgagcg tagtaaaaaa ctcggtgact
      421 gaaatatcca tactgacccc gcaaagtttg ccctgactca gtcccaaaga tttaggagca
      481 ttgatcatgg aaaaaagaac cggtgtattt ttcagatgtt ccctcgcagt ttttgtagca
      541 gagggtccga ttgtaatgat aaaaggagga ttttcatctt ctattttttt gaagtagttt
      601 gagatctcag gattttctgc tatgacgata tcgagatagg aaaccttcac atcggtgcct
      661 atgatcgact gaataccgta aagcccctgc tcatagatac ggttgtcaga agagatcagg
      721 atctcaatcg gtatattctt tttggattca gaatacagtg aatgaactga aaaaacaaac
      781 agtagaatca gaaatttcat attttttcca ctccggatac tccatttaat gctatcttat
      841 caatcttttc cagattgaat gttgcaggag caaagtcctc ttctatctcc aaggcctttt
      901 tccattccaa ttttgccttt tccggataac cgagcttata aaatacaccg cccagattat
      961 tgtatgcatg gatattcaac ggatcctcac tgatggaacg gttgtaataa atgatggcct
     1021 tttcataatt tccaaggaaa ctgttgcagt ttgccacttt cagaagaagc ttcggagatg
     1081 cggagtagct ttcatagtcc atcagagttt catactgatc caatgcctga agataatttt
     1141 tggctttgta gagagtctct gccttcctgt aaataaattc tgccttttca tccggtgcat
     1201 cctcattgga tctggccttc cgatgttcct tggcttgagc cagttcaatt ttcagcctgg
     1261 ctgcctcatc gttttcaggt tctattccaa gaagtctttc aatcatattt tccgatcttc
     1321 caaaaacatg catctgaagg tagattcttc ccaaattcag caggttttta ggatttgtct
     1381 gatgacgcag gaagagtctt tctccgacgt ccgctgccaa attcagttta ccggaaagcc
     1441 tgtaggcatc cgaaagaagg agcagatatt cattgtcccc cggcacaagt ttcagatact
     1501 gatcgtaaca ctcaattgcc ttggcaaact caccgactcc gaaatagtat ttccccagaa
     1561 ctttgagaag atcaggaaat tctctgtaat catccaggaa cttttcaatt ccatgatacg
     1621 cctcttccag ttctcccttt tcgagatgga caagactttt ctgaacgtcg gaacttgcct
     1681 ccggaggaag tttattgtcg gaaggaaggg cctcataact ttcattgaaa c
//
LOCUS       DAOQGJ010000945         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_49977_length_1730_cov_3.451940, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000945 DAOQGJ010000000
VERSION     DAOQGJ010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49977_length_1730_cov_3.451940"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK683_13865"
     CDS             complement(<1..251)
                     /locus_tag="PK683_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09581.1"
                     /translation="MIKKVLLPALLILMSCQMSSDVRIRTVPASGYDAYYNSEYIGRT
                     PVSLRASNYFGSDHEIEFRSKNLFLKREKLRLEWKPFNV"
     gene            complement(362..1162)
                     /locus_tag="PK683_13870"
     CDS             complement(362..1162)
                     /locus_tag="PK683_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH09582.1"
                     /translation="MRSYTKSIISIFLTLLGACKFPAQEGIPSNIKLLFSYAGAVEPK
                     VSSVLPKDKSTGSARNTQIVIVMNTEMDVSSLSSALTVKSDTETVSGDISVDGKMISF
                     VPKAYLGSTSKHTITIGTGAKSLKNQSPSSAISLTFSTGSEVDLTPPSISNTSPADSS
                     TGFPINAAILATYSETINPASFKADMVTVSNGSIAGTAELSESTISFKPAANLPANTV
                     LSVRIAAGLKDMAGNKISSPYTWIFTTGSKEAKVCVLGTDKYGDCLLF"
     gene            complement(1134..>1730)
                     /locus_tag="PK683_13875"
     CDS             complement(1134..>1730)
                     /locus_tag="PK683_13875"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNH09583.1"
                     /translation="SEFQKASEFLAKKAAGGIADYADIGIGAKGADSKYEAVFKSMAL
                     PGWGQYSYGTKSGSSWHKWKGIGFGSVFGLTVLNFAYRHTQYKSSQNNYSSVHNSFLI
                     MPAVTGAELAYFAKDRQAFAEYRKNGQAAEQAFLIIGAVYLMNLLDAYFTEKRILGLG
                     NMEKKTGFHLNADPVFIGAADTSLKKEINYEIVYKIYY"
BASE COUNT          434 a          398 c          370 g          528 t
ORIGIN      
        1 aatacgttga acggtttcca ttccagccga agtttttctc ttttcaggaa aagattcttt
       61 gaccggaatt caatctcatg atctgatccg aaataattgg atgcacggag agaaactggg
      121 gttcttccaa tgtattctga attataataa gcgtcatatc cagaggcagg tactgtcctg
      181 attctgacat cactgctcat ctggcaggac atcagtatca gcagtgcagg cagtaaaact
      241 ttttttatca tgatctttta tccatacttt cccgaatctc aagaaagtat ggctcatatt
      301 cctgccgagt gcaataggga gtttcagcat catcttaggg gttagggata atacctaaat
      361 gctaaaagag aagacagtct ccgtacttat cagttccgag aacgcagact tttgcttctt
      421 tggaacctgt agtgaatatc catgtgtatg gagaggatat tttatttcct gccatatctt
      481 tcaggcctgc tgcgattctg actgacagaa ctgtatttgc aggaagattt gctgccggtt
      541 tgaagctgat cgtgctttca ctgagttctg ctgttcccgc aatgcttccg tttgagaccg
      601 tcaccatatc tgccttgaag cttgccggat tgattgtttc gctgtaggtg gcaagaatcg
      661 ccgcattgat cgggaatccg gtgctggaat ctgcaggaga agtattggat attgagggcg
      721 gagtcagatc cacttccgat ccggtggaaa atgtaagtga gatcgcagaa gaaggtgact
      781 gatttttcag actttttgct ccggttccga tcgtaattgt atgcttgctt gtacttccga
      841 gataggcctt aggcacaaag gatatcattt ttccgtctac actgatgtct ccggaaactg
      901 tttccgtatc agactttact gtaagtgcag aagacagact ggaaacatcc atttccgtat
      961 tcattacaat tacaatctga gtgtttctgg cacttcctgt gctcttgtcc ttcggaagaa
     1021 ctgagctcac tttcggttcc actgcacctg catagctgaa gagaagcttg atatttgaag
     1081 gaattccttc ctgtgccgga aatttacagg ctccaagcag tgtcagaaaa atactaataa
     1141 tagattttgt atacgatctc ataatttatc tcctttttta atgatgtgtc agccgccccg
     1201 ataaaaacag gatctgcatt cagatgaaaa cctgtctttt tttccatatt ccccagtccc
     1261 agaatccgct tttctgtaaa ataggcatcc agcaggttca tcaggtaaac cgcacctata
     1321 atgagaaaag cctgttctgc cgcttgcccg tttttcctgt attctgcgaa tgcctgtctg
     1381 tccttggcga aataagcgag ttcagcacct gtcacagccg gcataatcag aaatgaattg
     1441 tggactgaac tgtaattatt ctgactgctt ttatattgcg tatgcctgta ggcaaaattc
     1501 agaaccgtca gaccgaagac agatccgaaa ccgatgcctt tccacttgtg ccagctgctt
     1561 cctgatttcg ttccataact gtactgaccc cagcccggaa gagccattga cttgaataca
     1621 gcctcatact tgctgtccgc cccctttgcg ccgatgccta tatctgcata gtctgcgatt
     1681 ccccctgcag ccttctttgc gaggaattca cttgctttct gaaattccga
//
LOCUS       DAOQGJ010000946         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50035_length_1729_cov_3.798686, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000946 DAOQGJ010000000
VERSION     DAOQGJ010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50035_length_1729_cov_3.798686"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="ftsA"
                     /locus_tag="PK683_13880"
     CDS             complement(<1..880)
                     /gene="ftsA"
                     /locus_tag="PK683_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNH09584.1"
                     /translation="MREELLESITALDLGSSLIKVVVGRPVNEHKIEIIGTGSAQCSG
                     IKQGAIINIDTTIRSIKEAIAQAELMAGIKIREVAVNISGKTIRSSNHKNVVAITNRE
                     RIVTEDDVQRVIDAILATTPIPSDQQIVHVLSKEFAVDDQTNIKEPLGMTGVRLEGEA
                     HIVTAGITTVHNLDKCIQSSGLIQMDKVLSSFASAEAVLTPSEKELGTAVVDIGAGII
                     DLVVYIEGGVAYSAVVPFGGSNVTNDITIGLKTPVEAAEIIKKKYGHTLRNAIDPTEK
                     IEVPAINGRPSRVILRQ"
     gene            complement(883..1605)
                     /locus_tag="PK683_13885"
     CDS             complement(883..1605)
                     /locus_tag="PK683_13885"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNH09585.1"
                     /translation="MQENKAGRIRTWLIFAVITALAVIVYEFSLDYLPFENGEIILSG
                     NKYLSKEEILQFIDVSEGKSIEKVDTKDWELRLKQNSRIRSVSVNRRSRSKILISVQE
                     RIPKYTVFSNGVLYELDEDWAIVSENDCRSRDILVLSGDFKPTENSFKEDVVLRDFAK
                     SVYKVLDIYPELKTRVSEFSKSEDGSIYLYLDTPKHLKIAIGTSLGSVQIRKMYSALA
                     YFETNKLTVNNLDLRGDDAVFQ"
BASE COUNT          459 a          448 c          320 g          502 t
ORIGIN      
        1 cctgacggag aatgactctc gacggtcttc cgttgattgc aggaacctct atcttttcag
       61 tagggtcaat tgcattacga agtgtgtgac cgtatttctt tttaatgatt tctgctgctt
      121 ccacaggagt cttcagtccg atagtgatat cattcgtcac attgcttccg ccgaaaggca
      181 caactgcaga ataggcaacc ccgccttcaa tatagacaac cagatcgatg attcctgctc
      241 cgatatccac aaccgcagtc cccagttcct tctcgctagg agtgagaact gcctcagccg
      301 atgcaaaact cgaaaggacc ttgtccatct ggatcagccc cgaagactga atgcatttat
      361 ccagattatg aacagtcgtg atgcctgcag tgacaatatg cgcttcccct tccagtcgga
      421 caccggtcat cccgagaggt tccttgatat tggtctgatc atccactgca aactctttgg
      481 agaggacatg cacaatctgc tgatcgctcg gaatcggagt cgtcgcaaga atcgcatcaa
      541 tgaccctctg cacatcatcc tcagtgacaa tcctctccct gtttgtaatt gctactacat
      601 tcttatgatt ggaagaacgg attgtcttcc cggaaatatt cactgccact tcacggatct
      661 tgatccccgc catcagttcc gcctgagcaa ttgcctcctt gatggaacgg atagtcgtgt
      721 caatattgat gattgctccc tgtttaatgc cggaacactg cgcagaccca gtgcctataa
      781 tttctatttt gtgctcattt acaggacggc cgacgaccac cttaatcagg gatgaaccca
      841 aatccaatgc tgtaatgctt tctaaaagtt cttctctcat gctcattgaa agactgcgtc
      901 gtcccctctt aaatccaaat tgtttacagt cagcttattt gtttcaaaat aggccaatgc
      961 agaatacatc tttctgatct gaacagaacc cagacttgtt cctatcgcaa ttttcagatg
     1021 cttaggcgta tccagataca gataaatact tccatcctct gattttgaaa attccgaaac
     1081 cctagtcttc agttccggat aaatatccag aaccttgtaa acagatttcg caaaatcccg
     1141 aagaaccaca tcttccttga acgaattctc agtcggctta aagtctccag aaagaactaa
     1201 aatatccctg ctcctgcagt cgttttctga tacaatagcc cagtcctcgt ccagctcata
     1261 aagcacgcca ttactgaaaa cagtgtattt aggaatcctc tcctgaacgg aaatgagtat
     1321 cttgctcctg cttctcctgt tgaccgaaac agatcgtatc ctcgaattct gcttcagcct
     1381 gagttcccaa tcctttgtat cgaccttttc aatcgatttt ccttccgaaa catcaatgaa
     1441 ctgcaggatc tcctctttgg aaagatactt gtttccgctg agaatgatct caccgttctc
     1501 aaaaggcaga taatcgaggg aaaattcata aacaatgaca gcaagtgcag taatcaccgc
     1561 aaaaatcagc caagttctga tccttcctgc tttgttttcc tgcaatgttt cgtccctcat
     1621 aataatatta tgtcttattc cttatcaaaa gtaactgatc agcatatatt tttatcggga
     1681 tttttgatgg gattttcaag ggcgaagccc ttgaaaccac cggtaggtc
//
LOCUS       DAOQGJ010000947         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50093_length_1728_cov_3.111178, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000947 DAOQGJ010000000
VERSION     DAOQGJ010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50093_length_1728_cov_3.111178"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PK683_13890"
     CDS             <1..391
                     /locus_tag="PK683_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09586.1"
                     /translation="FELERYQAEQQEDLNQFRLEKELERERQTMLLNLERAEKETETR
                     KIYHKEELEHLKNRLSAENTATPVNMEMEFNQNVLPKIADILASSMGEVKYNIFSGNE
                     KNMNPMAMILSDISDIFKARLRKNDSE"
     gene            423..875
                     /locus_tag="PK683_13895"
     CDS             423..875
                     /locus_tag="PK683_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09587.1"
                     /translation="MKKKFMIILLLLFSAGFIYAQDTAPEEPASTKGTSKKKIESKKE
                     MSLGEMVKILKGTTFDFGNGVTFKATDPMKKKGDYMRKGSSMGYTFGSFSKTGPNSAG
                     YFCPSLGNVEFTIHPDTITYKLNSVSLKNIKSHFKTAVPASQTVKRQK"
     gene            complement(829..1284)
                     /locus_tag="PK683_13900"
     CDS             complement(829..1284)
                     /locus_tag="PK683_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH09588.1"
                     /translation="MKEYRYKDLKIISERKIISDVGVRILIMEGRISNENSHLVSSDM
                     EREWCDGVFNAIADMNGLECINSGGLASILFMIEKCSENGGSFLIAGRNPYIQKIVEL
                     TGVNEQVRFFSSFAEAGNFFRSGSDMEIRSGLKRSTSVFSLSAMQALLF"
     gene            complement(1281..>1728)
                     /locus_tag="PK683_13905"
     CDS             complement(1281..>1728)
                     /locus_tag="PK683_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH09589.1"
                     /translation="IDRYYCLFECAPAGYIVLDRKLRLIEFNRTVLDIFGYSRSEMLL
                     RPVWHFIPVDFRSYFLLKCSSAAMDSAHRYADVQILGKDKERSWIRIHIFFSEPDNHY
                     FLNIFKTDELKGKEGELLQKVFECSFCERRTPAEETLHLENEKGAV"
BASE COUNT          553 a          392 c          336 g          447 t
ORIGIN      
        1 gtttgaactc gagagatacc aggctgaaca gcaggaagat ctgaaccagt tccgtctaga
       61 aaaggaactg gaaagggaaa gacagacaat gcttctgaat ctggagaggg cggaaaaaga
      121 aactgaaacc aggaagatct accataagga agaactggag catcttaaaa acagactctc
      181 agcagagaac acagccactc ctgtaaatat ggagatggag ttcaatcaga atgtgcttcc
      241 taagattgcg gatatccttg catccagcat gggagaagtg aagtacaata tattttccgg
      301 taatgaaaaa aacatgaacc ctatggcgat gattctttct gatatttccg atattttcaa
      361 ggcgagactt cgaaagaacg attctgagta gagatgccgc tgatataaaa ttggagaaca
      421 caatgaaaaa aaaatttatg atcattctgc tcctgctttt ttctgcagga ttcatatatg
      481 cacaggacac agcccctgag gaaccggcct cgacaaaagg aacttccaaa aagaagattg
      541 aatcaaaaaa agaaatgtca ctcggtgaaa tggtcaagat tctgaaagga accacattcg
      601 acttcggaaa cggcgtcaca ttcaaggcga cagacccgat gaaaaagaaa ggcgattata
      661 tgcgcaaggg aagttccatg ggctacacat tcggaagctt cagcaagacc ggaccgaact
      721 ctgcagggta tttctgtccc tcgctcggaa atgtggagtt cactattcat cccgatacaa
      781 tcacttacaa gctgaacagt gtgagcctga aaaacatcaa gtcccatttc aaaacagcag
      841 tgcctgcatc gcagacagtg aaaagacaga agtagaacgt ttcagaccgg atctgatctc
      901 catatcagac ccggatctga aaaaatttcc cgcctcagcg aagctcgaaa aaaatctgac
      961 ctgttcattc actccggtca gctcgacaat cttctgaata tacgggtttc tccctgcaat
     1021 cagaaaactg cctccgttct cactgcattt ttctatcata aaaagaatgg aagcaagtcc
     1081 tccgctgttg atgcattcca gaccgttcat atccgctatt gcattgaaaa ctccgtcaca
     1141 ccactctctt tccatatcag atgaaacaag atgagaattc tcattgctga tccttccctc
     1201 cataatcagg attctgacac cgacatcaga aattattttt ctttcagata taattttcag
     1261 atctttataa cggtattctt tcatactgcg cctttttcat tttccaagtg caaggtttct
     1321 tccgcaggag ttcttctttc acagaaactg cattcaaaga ctttctgcag aagttctccc
     1381 tctttcccct tcagctcatc tgttttaaaa atatttaaaa aataatgatt gtccggctca
     1441 gagaaaaaaa tatgaattct tatccaggac ctttctttgt ctttcccgag aatctgaaca
     1501 tctgcatacc ggtgcgctga atccattgca gcggaactgc atttcagaag aaaataactt
     1561 ctgaaatcca ccggtataaa atgccagact ggtcttaaaa gcatctcgga ccttgaatat
     1621 ccgaaaatgt ccaatacagt cctgttgaat tcaatcagcc tgagttttct gtccagaaca
     1681 atgtatcccg ccggtgcaca ttcgaaaagg caatagtatc tgtctatg
//
LOCUS       DAOQGJ010000948         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50169_length_1727_cov_2.581340, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000948 DAOQGJ010000000
VERSION     DAOQGJ010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50169_length_1727_cov_2.581340"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..402)
                     /locus_tag="PK683_13910"
     CDS             complement(55..402)
                     /locus_tag="PK683_13910"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNH09590.1"
                     /translation="MKLWIISIILQVLIVFFIFPVIDKEFRVSSDWKDSLVVVLLIAG
                     LNIIMKAALFILTVGIAWLFNKLTLGLAGLVLNSIILLWVSRMMPEKIKVPNFLSAFI
                     GAALLSAANALVF"
     gene            complement(454..1389)
                     /locus_tag="PK683_13915"
     CDS             complement(454..1389)
                     /locus_tag="PK683_13915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH09591.1"
                     /translation="MIQFVIFFAIFSISAFAEKNESAPKDVKTYTEMKYNSKSYKLRI
                     RAPELKQGNLAVMKIIPEEQSLGPENFKVTWMGADLRLEKYKESLVSLIPISPEASPY
                     DSLLVIEKKSGNETEIKKFRIPVEKTAFQTRKMYSELKVPTNFKTRKYSDETLTFIQK
                     CEEKKKAVFSAVNNFFANGKFVKPLKKIYVTSPFYVKRMYRPNDKGKPHGGIDFRGRK
                     GEKIYAIQDGKVAIAEKMFFEGNFTVIDHGLGMFSLYMHQSKMHVKEGEFVKKGTLIG
                     EVGSTGMSTGPHLHLGLKVNGTMVNPLKVIGLPLL"
     gene            1529..>1727
                     /locus_tag="PK683_13920"
     CDS             1529..>1727
                     /locus_tag="PK683_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNH09592.1"
                     /translation="MKKISGFRKGLTLVEIGVVTMILGLLIALSISSLTNMIRPSVAG
                     TVEKFQQALFFCWQSAILHNEV"
BASE COUNT          491 a          377 c          329 g          530 t
ORIGIN      
        1 agtttttttc cgcaggtatt gcatttatat tcataaaccg gcatttttaa ctccttaaaa
       61 aactaaagca tttgctgctg aaagaagcgc tgctccaatg aaggcagata aaaaattcgg
      121 aacctttatc ttttcaggca tcattctgct cacccaaaga agaataatcg aatttagaac
      181 aagacctgca agtcccagag tcagtttatt gaacagccag gcgattccca ctgtaagaat
      241 gaaaagagct gctttcatta taatattcag ccctgcaatc agcagaacaa cgaccagaga
      301 gtctttccaa tcggaactta ctctgaattc cttgtcgatg acagggaaaa taaagaatac
      361 aatcagtact tgcagaataa tagaaataat ccaaagtttc atagtaatat tttagactcg
      421 gcttcactcc tgcatagtaa aattttacag gatttacaga agtggaagtc cgatcacttt
      481 cagaggattg accatcgtac cgttgacttt cagtcccaga tgcagatgag gacctgtaga
      541 cattccggtg gaaccgacct cacctatcag agtgcccttc tttacaaatt ccccttcttt
      601 gacatgcatt tttgactgat gcatgtacag tgaaaacatt cccagcccat ggtctatcac
      661 agtgaagttt ccctcaaaga acattttttc tgctattgcc actttcccat cctgaatggc
      721 ataaatcttc tcccctttcc ggcctctgaa atcaattcct ccatggggct tgcccttgtc
      781 attcggtctg tacattctct taacgtaaaa aggactggtc acatagattt tcttcagcgg
      841 ttttacaaat ttcccattgg caaagaaatt attcacagca gaaaagactg cctttttctt
      901 ctcttcgcat ttctgtatga atgtaagtgt ttcatctgaa tatttgcggg ttttgaaatt
      961 agtaggaacc ttcagttcag agtacatctt tcgggtctga aaggcagttt tttcgactgg
     1021 gattctgaat tttttaatct cagtctcatt gccggatttt ttctcaatga ccagaagaga
     1081 gtcgtatggg ctcgcttccg gagaaatcgg aatcagtgaa acaagacttt ccttgtattt
     1141 ttcgagccgc agatccgcac ccatccaggt caccttgaaa ttttccggac ccaggctctg
     1201 ctcttcagga atgattttca tcactgccag atttccctgt ttcagctcag gcgccctgat
     1261 tctgagttta taactcttgg aattgtattt catttcagtg taggttttta catctttggg
     1321 agcggactca tttttttccg cgaaagcaga aattgagaaa atggcaaaaa aaataacaaa
     1381 ctggatcatc aatgacttcc tactaaggat acttactgaa aaatatggat tgttttttta
     1441 gtcaatcaaa actccctctg tcgtcggctg ctcgatcagc ttaaataaga cttgacagta
     1501 accaagtctg cagcagaata aacaggaaat gaagaaaatt tccggattca gaaaaggcct
     1561 tactctggta gagatcggag tagtgacaat gattcttgga cttcttattg cgctgtcgat
     1621 ttccagtctg acaaatatga tccgcccttc tgtagccggg actgtggaga aatttcagca
     1681 ggcgctcttt ttctgctggc agtcagcgat acttcataat gaagttg
//
LOCUS       DAOQGJ010000949         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50189_length_1726_cov_4.664273, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000949 DAOQGJ010000000
VERSION     DAOQGJ010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50189_length_1726_cov_4.664273"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PK683_13925"
     CDS             complement(<1..882)
                     /locus_tag="PK683_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09593.1"
                     /translation="MRPIFCILLTFLFMKCGVDRSPKLKFFMDTSAKSVSAVSSLASS
                     SFSVGGTVSGLNGTLFLSLNGTAESFSADGTYAFSSKLNSGSAYTVSVSSYPSGQNCS
                     VTNSSGTVTANVSNVDIACSTGNQYGALVGGTIINTLNLTGNLSTISSGAFYAGLNGI
                     TTDGSSLYLAVATNHTILKYDLASGSTSLLAGQSGVPGSSNGTLTGSTFNSPQNLIYY
                     GGYLYVAEYSGGNIRKLDITGNSVTTLQSGLSGPYGMTALNNCLYIGTIGAVKKIDLS
                     TNTISNFVGNGTAGYLDG"
     gene            complement(925..>1726)
                     /locus_tag="PK683_13930"
     CDS             complement(925..>1726)
                     /locus_tag="PK683_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09594.1"
                     /translation="WKESIIKRDTEKVESDLIGSLNTNLVQGAGIGLLTSLIQLIKQT
                     AVEKEDHYEISKDIINLLFENTKISEKLTNVLAEIDYIIRNPVKTISVSLKDIAELIE
                     KTADSLSSLALLKNHSIKTGKNSKLNDAQWVSLNSDFFGNAVKEILINAMKFSKPETS
                     VYVLYEIQKSSYLTVSFLSHPEVRENEPVGISQDYQNLIFEPFFRISKIVQEHYGTLD
                     FGLGLTQVQKTVKRFQGKVSAFNLKNSLDALSKELVCISIELPIKDIK"
BASE COUNT          483 a          362 c          352 g          529 t
ORIGIN      
        1 tccatccaga tagcctgcag ttccgtttcc gacaaaattg gaaatagtat tggtggacag
       61 atctattttt ttaactgcac caatcgttcc gatgtagagg cagttgttta aggcagtcat
      121 tccatacggg cccgacagac ctgactgaag agttgttaca gaatttccag tgatatccag
      181 ttttcgaata tttcctcccg aatactctgc cacataaaga taaccgccat agtatatgag
      241 attctggggt gaattgaatg tggatccggt taaagttcca tttgaactgc cgggaacacc
      301 tgactgccct gcaagcagac ttgtacttcc tgaagcaaga tcatatttta aaattgtatg
      361 gtttgttgca acagccaagt agagtgatga accgtcagtt gtaattccgt tcaggccggc
      421 atagaatgca ccgctgctga ttgtactcag attcccggtc agattcagtg tattgataat
      481 tgttcccccg acaagcgctc cgtactgatt tccagtactg caggcaatgt caacattgga
      541 aacattggca gtcactgttc ctgaagaatt tgtaactgag cagttctgcc cgctcggata
      601 agaggataca cttacagtat atgcagatcc ggaattcaat tttgaactga atgcatatgt
      661 gccgtctgca ctgaaacttt ccgcagttcc gttcagtgaa agaaaaagag taccgttcag
      721 tccggaaaca gtccctccta cagaaaaaga ggaagatgcc agtgaagaaa ctgccgacac
      781 tgattttgca gatgtatcca taaagaattt cagcttagga cttctgtcta cgccgcattt
      841 cataaaaaga aatgtgagca gaatgcagaa tatcggtctc atatttaagt tatggcagat
      901 actgacaaca gtgtcaataa cttttcattt tatgtcttta ataggaagtt caattgaaat
      961 gcagaccagc tcctttgata aggcatccag actgttcttc aggttgaatg cactgacttt
     1021 tccctgaaac cgtttaactg tcttctgcac ctgagtgaga ccgagtccaa aatccaatgt
     1081 accgtaatgt tcctgaacaa tttttgagat tctgaaaaac ggttcaaaaa tcagattctg
     1141 atagtcctga ctgattccca ccggttcatt ttctctgact tcaggatgac tgagaaagct
     1201 gacagtaaga tatgagcttt tctgaatttc gtaaagcaca tagacggaag tttccggttt
     1261 ggaaaacttc attgcattga tcagtatttc cttcactgca tttccaaaaa aatcagaatt
     1321 cagactgacc cactgtgcat cattcagttt cgaatttttc cctgtcttaa tcgaatgatt
     1381 tttaagtaat gccagtgaag acagtgaatc ggcggttttt tcaatcagtt ctgcaatgtc
     1441 cttcaggctg actgagatgg tcttgacagg gtttcggata atatagtcaa tttcagccag
     1501 aacgttcgta agtttttcag agatcttagt attttcaaaa agaagattta tgatatcttt
     1561 ggatatttca taatgatctt ccttttcaac tgcggtctgt tttatcagct gtataagaga
     1621 ggtcagaagt cctattcctg ccccctgaac gagattcgta ttcaggcttc cgatcaggtc
     1681 gctttccacc ttctcggtat ctcttttgat gattgattcc ttccag
//
LOCUS       DAOQGJ010000950         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50231_length_1726_cov_2.416517, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000950 DAOQGJ010000000
VERSION     DAOQGJ010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50231_length_1726_cov_2.416517"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK683_13935"
     CDS             <1..191
                     /locus_tag="PK683_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09595.1"
                     /translation="IAAASFILVAKDKPESSKKAVQFFSWTFQNGDEDAKKLIYVPLP
                     DELKTKVTAYWKENGLVN"
     gene            218..1153
                     /gene="pstC"
                     /locus_tag="PK683_13940"
     CDS             218..1153
                     /gene="pstC"
                     /locus_tag="PK683_13940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNH09596.1"
                     /translation="MSENKDQLTDRVVSVVTLLAALTVILLAAGIFLTLMKESIPAIR
                     KFGVFGFIFSTNWDPVNEDFRAGSAIFGTLITTSLALMIAAPASIGVAVFVTEIAPDR
                     LKNLIGLAIELLAAIPSIIYGMWGLYTLSPIMSKTVEPLLQNTFGRLPVFGVLFSGTP
                     LGIDILTASLILSIMIIPFSASIARDSFLLTPSVVKESSYALGATKWEMVIDVVMPYA
                     KSGVIGGIFLSLGRALGETMALAFVLGNKHKIPTSLFDAADTITVTLANEFAEAQGES
                     YLSSLFFLALILFFMSFTILAAAKILLKISQRKHR"
     gene            1153..>1726
                     /gene="pstA"
                     /locus_tag="PK683_13945"
     CDS             1153..>1726
                     /gene="pstA"
                     /locus_tag="PK683_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNH09597.1"
                     /translation="MSHQNWRKFKGFLALSLCSFAAAFGLFWLVFILGDVIRHGISAL
                     DLNLFMNDPAGPGNPGGGLRNAFVGQIMITSVSILIGVPMGILGGIFVAEYSRDTKLS
                     QTISILSDIMVSVPSIVIGTFAYAMFVKPVGRFNGWAGSIALSIIMIPVVLRTTEEML
                     KLVPSTLREAAYALGANKSQVTFQIVLRSAG"
BASE COUNT          444 a          397 c          385 g          500 t
ORIGIN      
        1 cgatcgccgc tgcatctttc attcttgttg caaaggacaa gcctgaatct tccaaaaagg
       61 cagttcagtt cttttcctgg acttttcaga acggtgatga ggatgccaaa aagctgattt
      121 acgttcccct gccggatgaa ttaaaaacta aagtaacagc ttactggaaa gaaaacggtc
      181 ttgtaaacta gatcaaacgt taaaagaatg ggaatgtatg tctgaaaata aagaccaatt
      241 gacagacagg gttgtctctg tcgtaacact gctggcagca cttactgtca ttctgctggc
      301 agccgggata tttttgacgc tgatgaaaga atcgattcct gccatcagga aattcggtgt
      361 ctttggattt attttctcga caaactggga tcccgtcaat gaggacttca gggcaggcag
      421 tgcgattttc ggcaccctga ttaccacctc attggccttg atgattgccg ctcctgcttc
      481 gatcggagtg gcagtcttcg tcactgaaat tgcaccggac agactgaaaa atctgatcgg
      541 tcttgccata gaacttctgg ctgcaattcc cagcatcatc tacggaatgt ggggacttta
      601 cacactctct cccatcatgt ccaagactgt ggaacccctg cttcagaata ccttcggcag
      661 acttccggtc ttcggagtcc tgttctccgg aactcctctg gggattgata tacttacagc
      721 aagcctcatc ctgagcatta tgatcattcc tttctctgcg agcattgcca gggactcctt
      781 tcttctgact ccctcagtcg tcaaagaatc ctcctatgca ctcggagcga ccaaatggga
      841 aatggtgatt gatgtagtga tgccttatgc aaagtcagga gtcatcggag gaattttcct
      901 gtccctggga agagcactgg gagaaacaat ggctcttgca tttgttctgg gaaacaagca
      961 caaaattccc acttctcttt tcgacgcagc cgacacaatc acagtcacac tggcaaatga
     1021 atttgcggaa gcacagggag agtcttattt atcatcatta ttttttcttg cactgatact
     1081 tttctttatg agttttacaa ttcttgcagc agcaaaaata cttttgaaaa tttctcaaag
     1141 gaagcacaga taatgtctca tcagaactgg agaaaattca agggttttct ggcactttcc
     1201 ctctgttcat ttgctgcggc cttcggactg ttctggcttg tgttcatttt aggcgatgtg
     1261 atccgtcacg gcatttctgc actggatctg aacctgttta tgaatgatcc tgcaggtccc
     1321 ggaaatccgg gcggcggact cagaaacgca tttgtcggtc agataatgat tacctccgta
     1381 tccatactga tcggtgttcc aatgggaatt ttaggaggca tattcgttgc ggaatattcc
     1441 agagatacaa agctgtctca gacaatcagc atactttccg atattatggt cagtgttcct
     1501 tccattgtga tcggaacatt tgcctatgca atgtttgtaa agcctgtagg aagattcaac
     1561 ggttgggccg gttccattgc actttccatc attatgattc cagtcgtact gagaaccacg
     1621 gaagagatgc tgaaacttgt tccttccaca ctgagagaag cagcttatgc tcttggagca
     1681 aataaatctc aggtgacttt tcagattgtg ctcagatcag caggaa
//
LOCUS       DAOQGJ010000951         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50232_length_1726_cov_2.391981, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000951 DAOQGJ010000000
VERSION     DAOQGJ010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50232_length_1726_cov_2.391981"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..954
                     /gene="cas6"
                     /locus_tag="PK683_13950"
     CDS             292..954
                     /gene="cas6"
                     /locus_tag="PK683_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HNH09598.1"
                     /translation="MRETGGKNERSSVLFLESAMTIVDLKFPVIGKKIPSDHGYLLYS
                     AISNLLPEVHNLEDISIRGISGVPDRSRGLNLGPASALNIRIDHSHIPVFLKLAGKEL
                     KIGDDKVRLKIPITKLLKPRSVLYSRLVTIKGFLNPMDFLDAVRRQLKAMGIEKETLL
                     FSTGSSVNPVRKTLRIHDKEIVGFPLVIPNLEPEESILIQTKGIGGRRKMGCGCFVGI
                     QI"
     gene            951..1193
                     /locus_tag="PK683_13955"
     CDS             951..1193
                     /locus_tag="PK683_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09599.1"
                     /translation="MKWRFERKGRAWDKDEAYEKYSLTPEKMEMIGGRLFWEEKHRLD
                     MLGLMLENVGIDKMLELFEDLSLLKEAIAERETGNG"
     gene            complement(1504..>1726)
                     /locus_tag="PK683_13960"
     CDS             complement(1504..>1726)
                     /locus_tag="PK683_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09600.1"
                     /translation="QDQQNKEKQDKKKNKKEQKNDEIKVTPSVTYVADIDLMRLLCPE
                     DVVKIKKAFDVFVSQYRQLILNKILQFFF"
BASE COUNT          540 a          300 c          394 g          492 t
ORIGIN      
        1 atggcaggga tgcctaccgg cagcagcttc ccaacttgcg ttggtttcgc aaaaattgaa
       61 gacgctgccg tcaggctgaa cagttacgct aaatttcaca catcacgcat atttttaagc
      121 caaaatattc tgtttgccta aaaatacaga caaatcattg tttccactgc ggcatactgg
      181 acggaaagga tagattcggt tccatttgta gaaaaaaagg aacatcaccc tccattttgt
      241 ccggggcttc agtagaatgg aaaaaatggc aggagttttt agaagaactc tatgagagag
      301 actggaggga aaaatgagag aagttcagtt ttatttttgg agagtgcaat gacaattgtt
      361 gatttaaaat tcccggtcat cgggaaaaaa attccgtcgg atcacggata tcttctgtat
      421 tcagcaattt caaatcttct tccggaagtc cacaatttgg aggatatcag cattcgtgga
      481 atttcggggg ttccggacag atccaggggt ctgaatctgg gacccgcgag tgctttgaat
      541 atcagaatag accattctca tattcctgtt tttttaaaac ttgccggaaa ggaacttaag
      601 atcggtgacg ataaagttcg tctgaaaatc ccaatcacaa aacttctgaa acctagatct
      661 gttttgtaca gcagactagt gacaatcaaa ggatttctga acccgatgga ttttctggac
      721 gccgtacgaa gacagctgaa agcgatggga atagaaaagg aaactctgct cttttccact
      781 ggcagttctg tcaatcctgt tagaaaaaca ttaagaattc atgataaaga aattgtcgga
      841 tttccgttgg tcataccgaa tctggagccg gaagaatcca ttctgattca gacaaaagga
      901 atcggcggaa gacgcaagat gggctgcggc tgctttgtgg ggatacaaat atgaaatgga
      961 gatttgaaag aaaaggcaga gcctgggaca aggatgaggc gtatgaaaaa tacagcctga
     1021 cccctgagaa aatggaaatg atcggaggcc gactgttctg ggaagagaaa cacagactgg
     1081 atatgcttgg tctgatgctg gaaaacgtcg ggatagacaa gatgctcgaa ctgtttgagg
     1141 atctgagtct actgaaagaa gcaattgcgg aaagggagac agggaatggg tagactttgg
     1201 gcaaagaaag acaatccatt gataaagaac aggaaggaat tatatttagt cccgcattta
     1261 aaggcagttg aatctatagg cagacttttg gttcgttcta tcggaaatcg gatattgcat
     1321 tctttcgatt tggattacaa tgtatggagt gaaagactga aagtgattct gcctctttcc
     1381 tgcttattcc atgatctagg taaagctaat gattggtttc agaaaatgat tcgaaataca
     1441 agcaatgaaa ggatacaaca accgattcga catgagttct tatctgtaat tctggtttta
     1501 cgatcagaaa aaaaattgca ggattttatt aagaatcaat tgtctgtatt gggaaacgaa
     1561 aacatcaaat gcttttttaa ttttaacaac atcttccggg cataataatc tcattaaatc
     1621 aatatcagca acatatgtaa cagatggagt gacttttatt tcgtcatttt tctgttcttt
     1681 tttatttttc tttttgtctt gcttttcctt gttttgctga tcctgt
//
LOCUS       DAOQGJ010000952         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50294_length_1724_cov_4.859197, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000952 DAOQGJ010000000
VERSION     DAOQGJ010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50294_length_1724_cov_4.859197"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PK683_13965"
     CDS             <1..427
                     /locus_tag="PK683_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH09601.1"
                     /translation="KERDSINDEIIIKKISDLRVLIIDDNRINLKVAKQLIAKIGVEA
                     SLASSGSEGLEMMKDCQFDFVFLDLQMPGMDGYEVSRRIRASGGINQDVIIIALTASI
                     ISEVKEKILSAGMNGMLTKPFKPEELQQILYKWTVRRRE"
     gene            540..>1724
                     /locus_tag="PK683_13970"
     CDS             540..>1724
                     /locus_tag="PK683_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH09602.1"
                     /translation="MSSYKPLFEESVSNPERFYGRYLDLIKWFKKPEKILSKDENGFF
                     RWFEGGRLNTSYLALDFHIENGRGSQTALIYDSPVTEQKIKYTYSELRDETAKFAGIL
                     KSFHVGKGDTVLIYMPMIPEAVIAMLACARLGAVHSVVFGGFAPHELAVRIDDSKPKV
                     IVSASYGVEIQKMIPYKPMLDEAIHKAEHKPENVIVCQRPGIQDYMHTGRDHYWQDLS
                     SDAVPAGYEEVNSTDPLYILYTSGTTGKPKGVVRDNGGHAVAMKLSMQAVYDMKPGDV
                     FWAASDVGWVVGHSYIVYAPLLYGCTTVLYEGKPVRTPDPGAFWRVISEHKVNLLFTA
                     PTALRAIRKEDPNGEYKKKYNLESLKYLFLAGERTDPPTYEWAADLLKIPVIDHWWQT
                     ETG"
BASE COUNT          507 a          331 c          424 g          462 t
ORIGIN      
        1 gaaagaaaga gattcaataa atgatgaaat tataattaag aagatttctg acctcagggt
       61 tctgattata gacgacaacc ggataaattt gaaggtggca aaacagctga tcgcaaaaat
      121 aggagtggaa gcctcacttg cctcttccgg ttcagaggga ctggaaatga tgaaggactg
      181 tcagtttgat tttgtatttc tggatcttca gatgccaggg atggacggtt atgaggtcag
      241 ccgcagaatc agagcttccg gagggattaa tcaggatgtg attatcattg cactgactgc
      301 ctccattatc tcagaggtaa aagaaaaaat cctcagtgca ggaatgaacg ggatgctcac
      361 taaacctttt aagccagaag agcttcagca gatactgtac aaatggacag taagacggag
      421 agagtgagaa aaatcatagt cttctgaatt gagctttcgt tttttttctt ttcatgcttt
      481 ctgcatacgg gaatataaga atctgcagac aatctgcaaa acctgacagg gagccttata
      541 tgagcagtta taaacctcta tttgaagagt cggtctcgaa tcctgagaga ttctacggca
      601 gatacctgga tctgatcaaa tggtttaaaa aacctgagaa aattctttcg aaagacgaaa
      661 acggtttttt ccgatggttt gaaggaggca ggctcaatac ctcctatctt gcacttgact
      721 ttcatattga aaacggaaga ggctcgcaga ctgctctgat ttatgattcc cctgtcactg
      781 aacagaaaat taaatacact tattctgaac tgagggacga gactgcaaag tttgcaggca
      841 tcctgaaaag ttttcatgtc ggcaaagggg atactgtact gatctatatg ccgatgattc
      901 cggaggcagt cattgcgatg cttgcctgtg caagactggg tgcagtgcac tctgtcgtat
      961 tcgggggatt tgcgcctcat gagcttgctg tccgaataga tgattccaaa ccgaaagtga
     1021 ttgtatcggc atcctacgga gtggagatac agaaaatgat tccttacaaa ccgatgcttg
     1081 acgaggcaat tcacaaggca gagcataaac ctgaaaatgt gatcgtgtgt cagcgtcctg
     1141 gaattcagga ttatatgcat actggaagag atcattactg gcaggatctt tcctccgatg
     1201 ctgtgccagc aggatatgaa gaagttaatt ctactgatcc tttatatata ctgtatacct
     1261 caggaactac aggaaagcct aaaggggttg taagagacaa tggcggacac gctgttgcaa
     1321 tgaagctcag tatgcaggca gtatatgata tgaagccggg cgatgtattc tgggccgcat
     1381 ccgatgtggg ctgggtagtc ggtcattcct acattgtgta tgctcctttg ctttatggat
     1441 gcactacagt tctgtatgaa gggaagccgg tacggactcc tgatcctggc gcattctgga
     1501 gagtgatttc agagcataag gtgaatcttc tttttactgc tccgactgca ctccgtgcaa
     1561 tcaggaaaga agacccgaac ggtgaatata aaaagaaata taatctagag tctttaaaat
     1621 atctgttttt ggctggggaa agaactgatc ctccgaccta tgaatgggct gccgatctcc
     1681 tgaaaatacc ggtcatcgat cattggtggc agacagaaac cggt
//
LOCUS       DAOQGJ010000953         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50356_length_1723_cov_3.643885, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000953 DAOQGJ010000000
VERSION     DAOQGJ010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50356_length_1723_cov_3.643885"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1637
                     /locus_tag="PK683_13975"
     CDS             213..1637
                     /locus_tag="PK683_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09603.1"
                     /translation="MTGVAVLRITYLYSDLKKKSKELEENNKVLDDLNQNLENKIEER
                     TKELAAAKRKTENLSSLIKDLNETSGIKNIMQMISDYVSEKFDLPYHSLFILNPQENR
                     FKFVHAVFPDYVPEDFKRKIELSSYSLDAENQESIHSSAIRNKVPIFIPDAEKEIKTE
                     AGKILISILKHKSFLTIPIFLQNSPIGTLDFFSLEKIQLDSEQIAEISLLTEHLAGVI
                     QNSLLFKEVQEEKEKAERERQKSDKLLLNILPKDVAAELKENGFAEPVLFENVSVMFT
                     DFKGFTSIAEKLSPQELVKDLDACFVQFDKISERYNLEKLKTIGDSYMCAGGIPRTNA
                     THAIDCILAALEIQDFMNLMKSLKEERGFPYWELRLGIHSGALIAGVIGEKKFAYDVW
                     GDTVNTASRMESSGTPGKINISGITYELVKEYFECDYRGKVNAKNKGEVEMYYVNGLK
                     LEYSKDGNGRTPNGKFWENYGYGN"
BASE COUNT          587 a          307 c          364 g          465 t
ORIGIN      
        1 tgattccatt cgatattttc ctgatcttca gctttctgca ttatatttat caggtgatcg
       61 tatactcata cagaggaaac aaagaagcca gaattatcgg aatagggata atccttctcg
      121 tatctttcgc acttcacgac attctggttg ttctgcgaat tgtggacaat attatgctga
      181 tgcatctcgg aactctttcc ttcattcttt ctatgacagg cgtagcagtt ctcagaatca
      241 cttatctgta cagtgatctg aaaaagaaat ccaaagaatt agaagaaaac aataaagtat
      301 tagatgatct gaatcagaat ctggaaaata aaatagaaga acggacaaaa gaattagcag
      361 cggcaaaacg aaaaacagag aacctgagca gtctgattaa agatctgaac gaaacctccg
      421 gcataaagaa tataatgcag atgatttcag actatgtaag cgagaaattc gatcttccct
      481 atcacagttt attcattctg aatccgcagg aaaacagatt caagtttgtt catgctgttt
      541 ttccagacta tgtccctgaa gactttaaaa gaaaaataga attgagcagt tactctctgg
      601 atgcagaaaa tcaggaaagc attcattcca gtgcgatacg aaataaggtt cccatattta
      661 tccctgatgc agaaaaagaa ataaagacgg aagccggaaa aattctgatc agtattttaa
      721 aacataaatc atttcttaca atccccattt ttctgcagaa ttccccaatc ggaactttgg
      781 attttttttc gctggagaaa attcagctgg attcagaaca aatcgctgaa atttctttac
      841 tgacagaaca tctggcaggt gtgattcaaa actcacttct attcaaggaa gtgcaggagg
      901 aaaaagaaaa ggcagaaaga gagcgtcaaa aatcagataa gctcctgctt aatattctgc
      961 ctaaggatgt ggctgctgaa ctgaaagaaa atggttttgc agaacccgta ctgttcgaga
     1021 atgtcagtgt gatgtttacg gactttaaag gatttacgtc aattgcagag aaactcagtc
     1081 ctcaggagct tgtaaaggac ctcgatgcct gttttgtgca attcgataaa atcagtgaga
     1141 gatataatct ggagaaactc aaaacgatag gagacagcta tatgtgtgcc ggcggaattc
     1201 cgagaacgaa tgccactcac gcaatagatt gtattttagc tgctctggaa atccaggatt
     1261 ttatgaatct aatgaaaagt ctcaaagaag aaagaggttt cccttactgg gaacttcgac
     1321 tgggaattca ttcaggagca ctgatagcag gtgttatagg agaaaagaaa tttgcttacg
     1381 atgtatgggg agatactgtc aacactgcca gccgaatgga atccagcgga acacccggca
     1441 agatcaatat cagcggcatt acttatgaac ttgtaaaaga atattttgaa tgtgattacc
     1501 gtggaaaagt gaatgctaaa aataaaggcg aagtagagat gtactatgtg aatggattaa
     1561 aactggagta ttctaaggat gggaacggga gaactccgaa cggaaaattc tgggaaaatt
     1621 acggatatgg taattagctt tcctgctgga tacttaagat caatctgcag ttcgattgtt
     1681 cagccccctg tcaaaatcct tgaaaaccgg atcgagtccc gat
//
LOCUS       DAOQGJ010000954         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50453_length_1721_cov_4.214286, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000954 DAOQGJ010000000
VERSION     DAOQGJ010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50453_length_1721_cov_4.214286"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..543
                     /locus_tag="PK683_13980"
     CDS             76..543
                     /locus_tag="PK683_13980"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH09604.1"
                     /translation="MTNDGTKDLYLIFSLGERLFGLEVASLTEITSIQPISPLADVPD
                     YVKGVINLRNRIIPAIDMRRRLKMEEIPYHERTCILVVNIKNNTVCLLVDSVKEIQRI
                     SSDRLENTPVGEGMERSAILRSVTGHSGSVIMIVSLEHLTDEIVFRSAKEIVI"
     gene            556..1032
                     /locus_tag="PK683_13985"
     CDS             556..1032
                     /locus_tag="PK683_13985"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH09605.1"
                     /translation="MEISEINSSEDRYLVFGLEDRLYGIEINYLSEIIPVPQITEVPN
                     MKDYVTGVADLRGKVIPVFDTKIRLGMTSCEMEERTCLIVLNVRGIQIGFIADSVKEV
                     MEISKEEADPLQNMKEMEHSEFIHAAGRLKNDQIVILLNAEKMTAGEHCLSARSEK"
     gene            1029..>1721
                     /locus_tag="PK683_13990"
     CDS             1029..>1721
                     /locus_tag="PK683_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH09606.1"
                     /translation="MNQDEIESLKELVSEEMENLSSLENELVILDRQLTEKSDLSEET
                     VNTVFRMFHSIKGSSGFFSLPAVVRTAHEAESLLDIIRRNREKLSRNLIDLLMETKDL
                     LTEVFIQISEKYSDLGFEDRCENLISRLRKEKEELSRENFSEESSTENYGLFEDEGKK
                     EEESFGLFEENNDKMIKKNTSSPVGFSEGMRKKEVSDSPSGKAAADIRIGTEKLDRLL
                     DLIGELVIAESQV"
BASE COUNT          562 a          292 c          426 g          441 t
ORIGIN      
        1 gaatcattcc agaaaattgc agcgaaattc agactggaca attccaggat actgaatctg
       61 gactgagttt cggctatgac gaatgacgga accaaagatc tgtatctgat tttttcgctg
      121 ggggagagac ttttcggact cgaggtggca agtctgactg agatcacttc aattcagcct
      181 atctctcctc tggccgatgt gcctgattat gtaaagggag tcatcaatct caggaacaga
      241 atcatccctg caattgatat gcggcgccga cttaagatgg aagaaatacc ttatcatgaa
      301 agaacatgca ttcttgtcgt aaatataaaa aacaatactg tatgtcttct tgtagattct
      361 gtgaaggaaa tacagagaat cagttctgac agacttgaga atacgcctgt gggtgaaggg
      421 atggaacgaa gtgcaattct gagatccgtg acaggacatt caggctctgt aattatgata
      481 gtcagtctgg aacatctgac agatgagatt gttttcagat cggcaaagga gattgtaatt
      541 tagactgtca tgaatatgga aatttctgaa atcaattcct ctgaagacag atatcttgtg
      601 ttcggactgg aagacaggct ctatgggata gaaattaatt atctttctga gattattccg
      661 gttccgcaga tcaccgaggt gccgaatatg aaagattatg taacaggtgt cgccgatctg
      721 agaggaaaag tgattcctgt gtttgacaca aaaatcagac ttggaatgac ttcatgcgaa
      781 atggaagaaa gaacatgtct gattgttctg aacgtcagag gcattcagat cggattcata
      841 gcagattcgg taaaggaagt gatggaaata tcgaaagaag aagcagatcc tctgcagaat
      901 atgaaggaaa tggagcacag tgaatttatc catgcggcgg gaagactgaa aaatgatcag
      961 atagtgattc ttctgaatgc agagaagatg actgcaggag aacattgtct gtcggcgagg
     1021 tctgaaaaat gaaccaggat gaaatcgagt ccttaaagga acttgtatcg gaggaaatgg
     1081 agaatctttc cagtctggaa aatgaactgg tcattctgga caggcagctt acagaaaaat
     1141 cagacttgtc cgaggagact gtcaatactg tattcagaat gtttcattcg ataaaaggca
     1201 gttccggttt tttcagtctt cctgcagttg taagaacagc gcatgaggca gagtctcttc
     1261 ttgacatcat ccggagaaac agagaaaaac tcagtcgaaa tcttatagat cttcttatgg
     1321 aaaccaagga ccttctgact gaagtgttta ttcagatttc tgaaaaatac agtgatttgg
     1381 gatttgaaga caggtgtgaa aacctgatca gccgtctgag aaaagaaaaa gaggaactga
     1441 gcagggagaa tttttcagaa gagagcagta cggagaatta cggacttttt gaggatgagg
     1501 gaaaaaaaga ggaagaatcc ttcggtcttt ttgaagaaaa taatgataaa atgattaaaa
     1561 aaaatacatc cagtcctgtc ggattttctg aaggaatgag aaaaaaggaa gtctccgatt
     1621 caccttccgg aaaagctgcg gcagacatcc gcataggaac cgagaagctg gacagactgc
     1681 tcgatctgat cggcgagctt gtcattgcag aatcccaggt g
//
LOCUS       DAOQGJ010000955         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50529_length_1720_cov_2.416817, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000955 DAOQGJ010000000
VERSION     DAOQGJ010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50529_length_1720_cov_2.416817"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PK683_13995"
     CDS             complement(<1..309)
                     /locus_tag="PK683_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH09607.1"
                     /translation="MKKLLLLSAVLLGAGLSAQSKNPASKTVPPSSSETAAPSPEISA
                     QSNDDLVKSISQGDLGGVKRALKNGANANLKIKDITPLMMAAGKGSKEIIDILIENKA
                     D"
     gene            complement(329..>1720)
                     /locus_tag="PK683_14000"
     CDS             complement(329..>1720)
                     /locus_tag="PK683_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH09608.1"
                     /translation="EARTPARLKHPNIVEVIDVGVCEDRPFYIMEYASGGSLSDKLKE
                     YRDKGQQFPFKEAVEIIAKVLDALDYCHKNNLQSHRDIKPANIMFRSNGEPIIVDFGI
                     AKVAESSITRTRMTMGTANYMSPEQCQGRKDIDGRSDVYSVGIMLFELLAGELPFKGD
                     SGLSIMIKQVKEKLPSLNQRIKTAKFDRTDSDYVKNAAKIEKIIDTACSKNKKKRYET
                     AREFALELSALTGTKLNLTPTGVSSLQNTWGLVLAVLFLGLGIGGVFGYKVLTSKPKV
                     NIIIDTVPPGAKVINTETSEEEGSTPFTASKIQAKPYRYRLSLDGYKESEIEIDLKNV
                     SQTESRRVDLEKKHSTSTDTSTDTANPDENNSGLVSAAGLVWQSGDIKRMNWYSAVTY
                     CRNLGMRLPSKTELKTGYESGVKTLSRPCCEYWSSTPHESDSDKAYNIHMGKLESFYS
                     SKSNLFYVRCVAR"
BASE COUNT          389 a          401 c          367 g          563 t
ORIGIN      
        1 gtcagcctta ttttctatca gaatgtctat aatttccttg gaacctttgc ctgccgccat
       61 catcagagga gtgatatcct tgattttcag attggcattc gcaccgtttt ttaaagctct
      121 ttttaccccg cctaaatctc cttgggaaat gcttttgact aaatcatcat tgctttgagc
      181 tgagatttca ggtgagggag ccgctgtctc agaactgctg ggcggcacgg tttttgacgc
      241 cgggtttttg gactgtgctg aaagaccggc tccaagtaat actgccgaca gtaatagtaa
      301 ttttttcata atgcctcctg tatttgacct atctggccac acatcttaca taaaataaat
      361 tcgactttga tgagtaaaaa ctttccagtt ttcccatatg aatattgtat gccttgtccg
      421 aatccgattc atgaggtgtg gacgaccagt attcacagca ggggcgggac agtgtcttga
      481 ctccgctttc atatcctgtc ttaagttccg tcttggaagg aagtctcatt cccagattcc
      541 tgcagtaagt gactgctgag taccagttca ttcttttaat gtcccccgac tgccagacaa
      601 gtcctgcggc agacacgagg ccggaattgt tttcatccgg gtttgcagta tctgtagaag
      661 tgtcagtcga ggtgctgtgt tttttctcca gatccactct tctggattct gtctgagaaa
      721 catttttcag atcaatttct atttccgatt ccttgtaacc gtccagagaa agtctgtatc
      781 tgtaaggttt tgcctgaatc ttggatgcag taaaaggagt gcttccttct tcctctgaag
      841 tctctgtatt gatgaccttt gctcccggcg gcactgtatc tatgataata ttgaccttcg
      901 gtttgcttgt gagcacctta taaccgaaaa ctcctccgat tcccagtccc aggaaaagaa
      961 ctgcgagcac aagcccccat gtgttctgaa gcgaactgac tccagtggga gtgagattga
     1021 gtttagttcc agtcaatgct gaaagttcca gtgcaaattc cctggctgtt tcatatcttt
     1081 ttttcttgtt cttggaacag gctgtatcta tgatcttttc aatcttggct gcatttttta
     1141 catagtcaga atctgttctg tcaaatttcg ccgtcttgat tctctggttc aggctgggaa
     1201 gtttttcctt tacctgctta atcattatgg aaagtccact gtctcccttg aaagggagtt
     1261 cgcctgccag aagttcgaaa agcataatcc ctacagaata tacatcactt cttccatcga
     1321 tgtcttttct gccctgacac tgttcggggc tcatataatt tgcagttccc attgtcattc
     1381 tggttcgggt gatcgaggac tctgccactt ttgcaattcc gaaatccaca atgatcggtt
     1441 ccccgttgga acggaacatg atatttgccg gcttgatgtc cctgtgggac tgaagattgt
     1501 ttttatgaca atagtccagt gcatccagaa ctttggcaat gatctccact gcttccttga
     1561 aaggaaactg ctgacccttg tctctgtatt ccttcagctt gtcagaaaga gagccgccag
     1621 aggcatattc catgatgtaa aacggtctgt cttcgcatac gcctacatcg atgacttcaa
     1681 ctatgtttgg atgtttcagt ctggcaggag ttctggcttc
//
LOCUS       DAOQGJ010000956         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50601_length_1718_cov_3.141311, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000956 DAOQGJ010000000
VERSION     DAOQGJ010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50601_length_1718_cov_3.141311"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PK683_14005"
     CDS             <1..460
                     /locus_tag="PK683_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH09609.1"
                     /translation="VLLDQNGKIVNPDTNGKALPYVPENVYTIAGTIRGKEGYYIRAE
                     YQYIGMQYGSLSSYRNLKSPANPYPSVNGVSYDFVWNTKDQTPDGNTGVIPSYGLVNA
                     SMGYKHPEKKWSVFITGKNLQDRHYISGRLPQGIMPGPTRQINIGFSMEF"
     gene            595..>1718
                     /locus_tag="PK683_14010"
     CDS             595..>1718
                     /locus_tag="PK683_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH09610.1"
                     /translation="MKLFLIAVKTFRENLRDSKSFYTALLFPALLLLIFFNAFGGGGF
                     SDFLNIGLTDRSRSPESFELIQKIRSLKFENSPVFSVREGSDSDIFRLCIAEGRCSAA
                     AVIPEMYSADAGKGKSEIELYGDTASDLYSFTEGFAEQALLERLEELNGKKISAGYRI
                     SFHKKSGTSNDFLTGLPGILVFAVFFGVIFTSMILNREETSGSFLRIRMSGTGISHYL
                     SGVLIFQIFFSFLQIFTAISIIYILGFRMQGSILQTGFIILILNLPASGLGYAAAAFS
                     RNTSEAVNIGTALMLPPVFLSGSLFPMDTFPVNLFGYTVNILSVFPSSDAADLIRKIL
                     IFGDSLNLHGGQTIQYFIISTVYFLSGLFIYRYRLSSGRK"
BASE COUNT          469 a          361 c          372 g          516 t
ORIGIN      
        1 tgttctgctc gaccagaacg gaaagatcgt aaatcctgac acaaacggaa aagctcttcc
       61 atatgttcct gaaaatgtat atacaattgc aggaactatt cgcggaaagg aagggtacta
      121 catcagggca gagtatcagt acatcggaat gcagtacgga tctctttcct cttacagaaa
      181 tctgaagtct cctgcaaatc cttatccttc cgtgaacgga gtgtcttatg atttcgtctg
      241 gaatacgaaa gatcagactc ctgacggaaa cacaggcgtg attccttcct atggtcttgt
      301 aaatgcatca atggggtaca aacatcctga aaagaaatgg tcagtattca ttacgggaaa
      361 aaatcttcag gacaggcact atatttcagg aagacttcct cagggaatca tgcctgggcc
      421 gaccagacag atcaatatag gattcagcat ggagttctga atctgaatct cagattacag
      481 aaagaaatat tagatattcc taatacagcc ggaactgacc ggctgcttca taaaatgatt
      541 aattctgttt gatctttttc agcataagag aaaactctgt attcagagtt acagatgaag
      601 ttatttctaa ttgcagtcaa gacattcaga gagaatctca gagacagtaa aagtttctat
      661 actgcactcc tgtttccggc acttcttctt ctcatctttt ttaatgcatt cgggggaggg
      721 ggcttttccg actttctgaa catcgggctg actgacagat cgagaagccc ggagtctttc
      781 gaactcattc aaaaaatcag atcattaaag tttgaaaaca gtcctgtttt ttccgtcaga
      841 gagggatctg attctgatat cttcagacta tgcattgcgg agggaaggtg ttctgcggct
      901 gccgtcattc ctgaaatgta ttccgctgat gcaggaaagg gaaaatcgga aattgaactt
      961 tacggagaca ctgcatccga tctgtattct ttcacggaag gttttgccga gcaggctctt
     1021 cttgagagac tcgaggagct gaacggaaaa aagatcagtg cgggatacag gatatccttt
     1081 cacaaaaaga gcggaacttc caatgatttt ttgaccgggc ttccggggat tcttgtcttc
     1141 gctgtatttt tcggtgtcat attcacctca atgattctga acagagagga aacgtccgga
     1201 agtttccttc gaatccgaat gagcggaaca ggcatttctc attatctgtc gggagtattg
     1261 atttttcaga tctttttttc atttcttcag atatttactg caatttccat aatttatatt
     1321 ctcggattca gaatgcaggg gagcattctt cagactggat tcattattct cattttgaat
     1381 cttcctgctt cgggactggg gtatgctgcc gctgcttttt ccagaaatac cagtgaggcg
     1441 gtcaatatcg gaacggcact gatgcttccc ccggtcttcc tgtcaggctc cctttttcct
     1501 atggatactt ttcccgtaaa tcttttcgga tacaccgtga atattctatc cgtttttccc
     1561 tcctccgatg cagcggatct gatccgcaag attctgattt tcggggacag tctgaacctt
     1621 catggaggtc agacaatcca atattttatt ataagcacag tctattttct ttcaggactt
     1681 ttcatttaca gatacagact gagttcagga aggaagga
//
LOCUS       DAOQGJ010000957         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50662_length_1717_cov_2.709988, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000957 DAOQGJ010000000
VERSION     DAOQGJ010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50662_length_1717_cov_2.709988"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PK683_14015"
     CDS             <1..304
                     /locus_tag="PK683_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH09611.1"
                     /translation="DTSSSPANKASYLSRISFACRNLPVPNYEDAVKYLENAVQVSET
                     STDKATYYGMIATVYAEMEPAENAKALQYLEKAVSSAPGHYSAPQWKEMISSLKKR"
     gene            complement(367..1110)
                     /locus_tag="PK683_14020"
     CDS             complement(367..1110)
                     /locus_tag="PK683_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH09612.1"
                     /translation="MNLFDVSGKTVLITGASRGIGKSMALAFRDAGAVVYGTGSREET
                     VEWMKGEKIYGRAADISKAGTILPVIEEIKKSHGRLDCLINNAGIASNTPASAMKDDE
                     MYKIIDTNFTGMFRACQAYYKTHKLIGGNIINISSVLGLVGYSLASVYSGTKGAAIQL
                     TRALAAEWINNNFRLNAICPGFIDTDMNKMIKDKQNVFEKVSQGIPMKRLGSPEDISG
                     AAIFLASGASSYMTGQVITVDGGLTTVMS"
     gene            complement(1112..>1717)
                     /locus_tag="PK683_14025"
     CDS             complement(1112..>1717)
                     /locus_tag="PK683_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09613.1"
                     /translation="FIESSGAVSVSASYYRRILKLKKGARIRIRLPLYANHEKKMTVY
                     KFIEGRGWVEKGNDEIIETEEKKFRLFSGADELTHWVFGTPEQNTFCIEGNIISENPP
                     YSAVLIGSDFKTSHSIKEKKAEFRMQIPLQKKFRILIMDEFGNLGISREMSIQKEKPE
                     SGCRRAEEISVVTGSREIRKDRKKLLEYLGWKDIFQKETGE"
BASE COUNT          430 a          423 c          313 g          551 t
ORIGIN      
        1 ggatacatcc tcgtctcctg caaataaggc ttcttactta agccgaattt catttgcctg
       61 cagaaatctt cctgttccca actatgagga tgcagtaaag tatcttgaaa atgcagttca
      121 agtctccgaa acctcgactg acaaagcgac gtattatgga atgattgcaa ctgtctatgc
      181 ggaaatggaa ccggcagaaa atgcaaaagc tcttcaatat ctcgaaaagg cagtttcatc
      241 tgcaccaggt cattactctg caccgcagtg gaaagaaatg atcagcagtc tgaaaaaaag
      301 ataatcactt tttcattata ataaaatacg aggccgcaga aggcctcttc tgttttttta
      361 tcagtcctac gacattacag tcgtcagtcc cccgtccaca gtaattacct gaccagtcat
      421 atatgaagat gctcctgagg caagaaaaat ggcggctccg gaaatgtcct ccggactccc
      481 cagtcttttc atagggattc cctgagacac cttttcaaat acattctgct tgtccttgat
      541 catcttgttc atatcggtat caatgaatcc cgggcagatt gcattcagcc tgaaattatt
      601 gttgatccat tctgcagcaa gcgctcttgt cagctgaatc gcagcgcctt tggtgcctga
      661 gtagactgag gcaagagagt agcccaccag tccgagaacg gatgaaatat tgatgatgtt
      721 tcctccgatc agcttatgtg tcttgtagta tgcctggcat gcacggaaca ttcccgtaaa
      781 gttggtatca atgattttat acatttcatc gtccttcatt gcagatgcag gcgtattcga
      841 tgcaattcct gcattattga tcagacagtc gagtcttcca tggctttttt tgatttcttc
      901 aatgacggga agaattgtgc ctgccttgct gatatcagcc gctcttccgt atattttttc
      961 tcccttcatc cattctacag tctcttctct gcttcccgtt ccgtaaacga ctgcccctgc
     1021 atctctgaat gcaagagcca tggattttcc gattcccctg ctggcccctg ttatgagcac
     1081 agtctttccg ctgacatcaa ataaattcat ttcactctcc tgtctctttc tgaaaaatat
     1141 ccttccagcc cagatattcc aataatttct tcctgtcttt tctaatttcc ctgcttcctg
     1201 tgacaaccga aatctcttca gctctcctgc atcctgattc cggcttttcc ttctgaatgc
     1261 tcatttccct ggaaattccc agattgccga attcatccat tatgagtatt ctgaatttct
     1321 tctgcaatgg aatctgcatt ctgaattctg cttttttttc cttgatggaa tgagaagtct
     1381 taaaatcgga tccgatcagg acagccgagt atggaggatt ttccgaaata atatttcctt
     1441 cgatgcagaa ggtattctgc tcgggagttc cgaataccca gtgagtcagt tcatccgctc
     1501 cggaaaacaa tctgaatttt ttttcctcag tttcaatgat ttcatcattt cctttttcca
     1561 cccaccctct tccttctata aacttgtata ctgtcatttt tttttcatga tttgcataca
     1621 ggggcagcct gattctgatc cgtgctccct ttttcagttt cagtattctt ctgtaataag
     1681 aagcagatac agaaacagca cctgaagatt caatgaa
//
LOCUS       DAOQGJ010000958         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50664_length_1717_cov_2.521661, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000958 DAOQGJ010000000
VERSION     DAOQGJ010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50664_length_1717_cov_2.521661"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK683_14030"
     CDS             <1..369
                     /locus_tag="PK683_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH09614.1"
                     /translation="KIEEKVYEVFEEYYWPGNLRELQNVLQRMIIFSEKEFLSISDLP
                     PEIQNYNKITQTEKKKTGTDLKAEMTKINEDLSMRELEKEAILKALKKAENNKDLAAR
                     ILGISRASIYRKLKEYRIKN"
     gene            complement(359..1684)
                     /gene="murC"
                     /locus_tag="PK683_14035"
     CDS             complement(359..1684)
                     /gene="murC"
                     /locus_tag="PK683_14035"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002092985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNH09615.1"
                     /translation="MGAGGSGMSPLGHILLDIGLPLFGQDKNAGESFESLKERGMTAV
                     SSPEELEEIRPAAAVFSSAISRTENLFFRKCEELGIPLMHRSELLHRVFGSMKSVSVA
                     GSHGKTTTTAMTAQIFSECGLEPAVMVGGETAILGKKGGQFGKGLIGVYESDESDGTF
                     LNHSADVRIVTNVDNDHLDYYKELDNLFRSFGKHICGDGGGTAVLNLDEEGTLSALKY
                     CSNLKILGFSAEKTDPNSVFYKINGRRMNFRLKGTEYELEVPYPGDHYLKNALSAVLA
                     AFLCGIEIKRSVSILKNYRGVKRRLEYLGNAGDVPVYDDYGHHPTEIKSVISSLSQMK
                     KAGGRTAVVFQPHRYTRTKELYKEFAQVLQMADLLFLLPIYSAGEKPEEGISTELIYQ
                     LTDRNRTFLLKEGLEKDTEEIRKRLKEGDVFVSLGAGNVRNWGERLVSS"
BASE COUNT          484 a          408 c          336 g          489 t
ORIGIN      
        1 aagatcgaag aaaaggtata tgaagtattt gaagaatact actggccggg aaatctaaga
       61 gaactccaga atgttctgca aagaatgatt attttttccg aaaaggaatt tctttccatt
      121 tctgatctgc ctcctgaaat ccagaactac aataagatca cccagacaga aaaaaagaaa
      181 acaggaactg atcttaaggc tgaaatgaca aagatcaatg aggatctttc aatgagggaa
      241 cttgaaaagg aagccatact gaaagcctta aaaaaggcag aaaataacaa ggatcttgca
      301 gcaaggattc taggaatttc cagggcttcc atatatagaa aactgaaaga atacaggatt
      361 aagaactgac cagtctctcc ccccagtttc ttacattgcc cgctcccaga ctgacaaaaa
      421 catccccttc tttcagtctc tttctgattt cttcagtatc cttctccagt ccttccttca
      481 gaagaaaagt cctgttcctg tccgttaatt ggtagatcag ttcagtactg attccttcct
      541 caggtttttc tcctgcagaa taaataggaa gaagaaaaag cagatctgcc atctgaagta
      601 cctgagcaaa ctccttgtac agctctttcg ttctggtgta tctgtggggc tgaaaaacaa
      661 cggcagttct gcctcctgct tttttcatct gactgaggga tgaaatgacg ctcttgatct
      721 cagtcggatg atgcccgtaa tcgtcgtaca ccggtacatc gcctgcattg ccgagatatt
      781 ccagccttct tttaactcct ctgtaattct tcagaatgga aacagatctt tttatttcga
      841 ttccgcacag aaatgcggca agcactgctg agagagcatt tttcagataa tgatctcccg
      901 gatatggaac ttccagttca tactctgttc ctttcagacg gaaattcatt ctgcgcccgt
      961 ttattttata gaagaccgaa tttggatctg ttttttctgc tgaaaaaccg aggattttca
     1021 gattactgca gtattttaga gcagaaagag ttccctcttc atccagattc agcacggcag
     1081 ttcccccgcc atccccgcat atatgctttc caaaacttct gaacagatta tccaattctt
     1141 tataataatc aagatgatca ttgtctacat tagtaacaat gcgcacgtcc gcagaatgat
     1201 tcagaaaggt cccgtccgat tcatctgatt catatacgcc gatcagccct ttcccgaact
     1261 gcccgccttt ctttccgagg attgcagttt cacctccgac cattactgca ggctcaagtc
     1321 cgcactcgga aaaaatctga gcggtcattg cagtggttgt agtctttccg tgtgatccgg
     1381 caactgaaac tgatttcatg cttccaaata ctctatggag aagttcagac ctgtgcataa
     1441 ggggaatacc cagttcctcg cattttctga agaacagatt ttcagtgcgg ctgattgcac
     1501 ttgaaaacac agcagccgct ggacggattt cctccagttc ctccggcgag gatactgctg
     1561 tcattcctct ttctttcaga ctttcaaatg attctcctgc atttttgtcc tgaccgaaaa
     1621 ggggcagacc gatatcgaga agtatatgac cgagaggaga cattcctgat cctccggctc
     1681 ccatgataaa agtcttcttg cggatatcag acaggct
//
LOCUS       DAOQGJ010000959         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50755_length_1715_cov_2.903614, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000959 DAOQGJ010000000
VERSION     DAOQGJ010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50755_length_1715_cov_2.903614"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK683_14040"
     CDS             <1..234
                     /locus_tag="PK683_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH09616.1"
                     /translation="NNAYSSPYSYPQYRPKTVPAWISVNLRISHKISDNIQLGLYITN
                     LFNTHQTLVQRSNYPFDYIREGRRVMIDFQAAF"
     gene            300..>1715
                     /locus_tag="PK683_14045"
     CDS             300..>1715
                     /locus_tag="PK683_14045"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNH09617.1"
                     /translation="MIKSKFIFITGGVCSSLGKGVTVAALGCLLESRGYSVALQKMDP
                     YINIDPGTMSPYQHGEVYVTNDGAETDLDLGYYERFTSQEFSRKNSITTGQIYHSVIE
                     RERKGDYLGRTVQVVPHITNEIRSRIYALGKDVEPDFIIVEIGGTVGDIESIPFLEAI
                     RQMKYEHGNEQSLFIHLTLVPTITVAGEAKTKPTQHSVKELLGLGIQPDILVCRVKDR
                     MSKEMKSKISLFCNVRDECVISASDIEDSIYEIPKMYKEEKLDEVVLKALKMPLKKSN
                     FAEWDRMVQKIKTASKKVNIAVVGKYISLQDAYRSIYESLSHGGIANEVHVDFVKINP
                     EDLEKGNPKDFFKNIQGILVPGGFGERGIEGKISAINYARTKGIPFFGICLGMQCAVI
                     EFARNVLGLKNAHSTEFVPNSPYPVISLLEEQADVERMGGTMRLGAYPCIIKKGTVAY
                     SEYKQERTMERHRHRFEFTLRF"
BASE COUNT          552 a          342 c          381 g          440 t
ORIGIN      
        1 aacaatgctt acagcagtcc ttattcctat cctcagtacc gcccgaagac tgtgccggca
       61 tggatcagtg tcaatctcag gatcagtcat aaaatttctg ataatattca gcttggactc
      121 tatattacaa atcttttcaa tactcatcag actcttgtac aaagatcaaa ttatccgttt
      181 gattatatca gggaaggaag aagagtaatg atcgattttc aggccgcttt ttaagaggaa
      241 ttccttcaaa aaaaaccttt acttcagact gtctgaagaa actgtacaaa cagaaagcca
      301 tgattaaatc aaaattcata ttcattacag gcggagtctg ttcttcgctc ggtaaagggg
      361 ttaccgttgc tgccctaggc tgtcttctcg aatcgagagg atactcagtt gctcttcaga
      421 aaatggatcc ttatatcaat atcgatcccg gcacaatgag tccttatcag catggagaag
      481 tatacgtcac gaacgacggt gcagagacgg atctggatct gggttactat gaaagattta
      541 ccagccagga attttccaga aaaaactcaa tcactacagg gcagatttat cattcagtca
      601 tagaaagaga aagaaagggt gactacctgg gtcggacagt tcaggtagtg cctcatatca
      661 caaatgaaat cagaagcagg atttatgctc tgggaaaaga tgtggaacct gatttcatca
      721 ttgtggaaat cggagggacc gtcggcgaca ttgaatcaat acctttcctc gaagcaatcc
      781 gtcagatgaa atatgaacac ggaaatgaac agtctctttt tatacatctt acacttgtcc
      841 ccacaatcac tgttgcaggc gaagcaaaga caaaacctac tcagcactca gtgaaggaac
      901 ttctcggtct cggaattcag ccggatattc ttgtgtgcag agtcaaggac aggatgagca
      961 aggaaatgaa gtctaaaata tcattattct gtaatgtaag agacgaatgt gtaatttccg
     1021 cttccgatat agaagattcc atctatgaaa ttccaaaaat gtacaaagag gaaaaacttg
     1081 acgaggttgt actgaaggca ctgaagatgc ctctgaaaaa gtccaatttt gcagaatggg
     1141 acagaatggt tcagaaaatc aagactgcgt caaagaaagt gaacattgca gtggtgggaa
     1201 aatatatcag tcttcaggac gcatacagat ccatttatga atctctgtct cacggaggga
     1261 ttgcaaatga ggtgcatgtg gactttgtca agatcaatcc tgaggatctg gaaaaaggaa
     1321 atccgaaaga ttttttcaag aacattcagg gaattcttgt accgggggga ttcggtgaaa
     1381 gaggaattga aggaaagatc tccgccatca attatgccag aaccaaaggc attccctttt
     1441 tcggaatctg tctcgggatg cagtgtgcag tcattgagtt cgcaaggaat gtgctcggat
     1501 taaaaaatgc tcactccact gaatttgttc ccaattcccc gtaccctgtc atttctcttc
     1561 tggaagaaca ggcggacgtg gaaagaatgg ggggcacaat gagactgggt gcatacccat
     1621 gcatcatcaa aaaaggaacg gtcgcatatt cagagtataa acaggaaaga acaatggaaa
     1681 ggcacagaca ccgtttcgaa tttactctcc gcttc
//
LOCUS       DAOQGJ010000960         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50759_length_1715_cov_2.754819, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000960 DAOQGJ010000000
VERSION     DAOQGJ010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50759_length_1715_cov_2.754819"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PK683_14050"
     CDS             <1..888
                     /locus_tag="PK683_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH09618.1"
                     /translation="PSSGQFREPFEIGQILSDMGIKYDAVDLTIRYYKERAIPYLVPF
                     PVKDIALAGEPIMEGTDLWDVSSPMENINWFQSALKSPVPIPGYTLVEDFFGMDTGRE
                     KTKEPVDLDIYVDCSGSMPNPGYNISYLALAGTIICISALRVGARVQATLWSGTGEYH
                     TTKGFIRDEKEILKVVTGYIGGATAFPLNLLRKTYLESYKHDRKSHILHISDEGIDTM
                     LDKDEKGGKGTEITEESLKNAGGGGTMVLNLYSPAESNAKLKKIAKMGYKIHRVNDWE
                     SLVKFAYEFSKEKYTEAGK"
     gene            885..1457
                     /locus_tag="PK683_14055"
     CDS             885..1457
                     /locus_tag="PK683_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09619.1"
                     /translation="MKEGPELIELLDRLMDCPKEFYSSDRSYRSETENKALFSDLHFF
                     LTGKYPDEKMLALIPSDKDENLGRCASMCTYILFHQWFRNRKEAAELSAMFLSSVLPA
                     YSSAVSADEFAGNSERREELIRLLLYHLGYFPKGETEKYSLNRLAALDSVERDKVIKE
                     SREVMKKVQALREAMARKAAEEAASKMTGE"
     gene            1460..>1715
                     /locus_tag="PK683_14060"
     CDS             1460..>1715
                     /locus_tag="PK683_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09620.1"
                     /translation="MLLDELRERIPLIEEENLKLYTELQNHSSAPSWNYVCGDRITEN
                     DRKFIEDFSEKLQSDRRMMSEPDDSILKWILEKEKASPHFR"
BASE COUNT          533 a          358 c          413 g          411 t
ORIGIN      
        1 ccttcatccg gtcagttcag agaaccgttt gaaatcggtc agattctttc tgatatggga
       61 atcaagtacg atgctgtgga tcttacgatc cggtactaca aggaaagggc aatcccctat
      121 ctcgtccctt ttccggtgaa ggatattgct cttgcaggag aaccgatcat ggaaggaaca
      181 gacctttggg atgtaagctc tcctatggaa aatatcaact ggtttcagtc ggctcttaag
      241 agtcccgttc ccatcccggg atatacactc gtggaagatt ttttcggaat ggataccggc
      301 agggaaaaga caaaggaacc tgtcgatctg gacatctatg tggactgttc cggctccatg
      361 cctaatcctg gatataatat atcctatctg gccctcgcag gaacaatcat ctgcatctca
      421 gcccttcgag tcggagcccg tgtccaggcg actctctgga gcggaaccgg ggaatatcat
      481 actacaaagg gattcatacg tgatgaaaag gaaatactga aagtagtcac aggctacatt
      541 ggaggtgcga ctgcatttcc gctgaacctt ctccgtaaga catatctgga atcctataag
      601 catgacagaa aatcccatat cctgcatata tccgatgaag gaatcgatac tatgctggac
      661 aaggatgaaa aaggcggaaa gggtacagag attactgagg aatcactgaa gaatgctggg
      721 ggcggcggca caatggttct gaacctgtat tcacctgcag aatccaatgc aaaactgaaa
      781 aagatcgcca agatgggtta taagatccac agagtcaatg actgggaaag cctcgtcaaa
      841 tttgcatatg agttttcaaa ggaaaaatat acggaggccg ggaaatgaaa gaaggaccgg
      901 aactgatcga acttctggac agacttatgg actgtccgaa ggaattctat tccagcgaca
      961 gatcttacag gtctgaaacg gaaaacaagg ctcttttcag tgatctgcat ttttttctca
     1021 ctggaaaata tcctgacgaa aagatgcttg ccctgatacc ctcagacaag gatgagaacc
     1081 tgggcagatg tgcttctatg tgcacatata ttctatttca tcaatggttc agaaacagga
     1141 aagaagctgc tgagctttct gcaatgtttc tgagttcagt tcttcctgca tattcctctg
     1201 cagtcagtgc agacgaattt gcaggaaact cagaaagaag agaagaactg atacggcttc
     1261 tgctctatca tttgggatat ttccccaagg gtgaaacaga aaaatacagt ctgaacagac
     1321 ttgctgccct tgactctgtg gaaagggaca aggtaatcaa agagagcaga gaggtaatga
     1381 aaaaggttca ggcactcaga gaggcaatgg caagaaaagc tgcagaagag gctgcatcca
     1441 aaatgacagg ggaataaatt tgctcctcga tgaactgaga gaaaggattc ctctgatcga
     1501 ggaagaaaac ctgaagctgt acactgaact tcagaaccac agttctgcac cctcatggaa
     1561 ctatgtctgc ggtgacagga taactgagaa tgacagaaag ttcattgagg atttttcaga
     1621 aaaacttcaa tcagacagaa ggatgatgtc tgagcctgat gattccatcc tcaagtggat
     1681 tctggaaaaa gaaaaggctt ctcctcattt cagaa
//
LOCUS       DAOQGJ010000961         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50767_length_1715_cov_2.586747, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000961 DAOQGJ010000000
VERSION     DAOQGJ010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50767_length_1715_cov_2.586747"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1715)
                     /locus_tag="PK683_14065"
     CDS             complement(<1..>1715)
                     /locus_tag="PK683_14065"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNH09621.1"
                     /translation="SDKSEQFFGFGEQYTHFNMKGKTPFIFTEEQGIGRGDQPITFGA
                     NLTADAGGNEYTSYAPMPFYISTKNRSVFFENSSYSKFDFSNENLVKVQFWEGNLKGS
                     VWIGETPEELISQFTEKTGRYSELPDWAYGTWMGLQAGEITAPALGEKQLKGYDKINE
                     ILNDAKAAGNPVTALWIQDWCGRRITGFGDQLKWRWYADEEMYPKFAEFVKTMNKKNI
                     QVLGYINSFLADRDPKKADEKFENPMLEEAKAKGYLVKNQDGKDYLIQTVGFPAYLID
                     LTNPEAAKWTKSIIKKNMIDVGLAGWMADFGEWLPFDAKLHSGISAEVYHNQYPVDWA
                     RVNREAIAEAGKEGKIVFFTRAGYSYSGKYSTAFWLGDQMVSFGENDGIASTVVGLNS
                     GGISGIALNHSDIGGYTTIDNPLMNYHRSKELFLRWTELNAFTPIFRTHEGNKPKRNH
                     QPYTDDDTVKFFARFGKIHLALKPYLQSLAAEAKKTGMPVVRHPYIHYPNDKNTYNLK
                     HQFLLGKDLLVLPVVKKGADSVEGYFPEGEWTHIFTGKKYPGGKFHDVDAPLGTPAVF
                     AKTGG"
BASE COUNT          422 a          427 c          329 g          537 t
ORIGIN      
        1 ctcctccggt tttagcaaaa acagcaggcg ttccgagcgg tgcatccaca tcgtgaaact
       61 tccctcccgg atattttttc cctgtaaata tatgcgtcca ttctccttcc ggaaaataac
      121 cttccacact gtccgctccc ttcttcacga ccggcagaac aagaagatct tttccgagaa
      181 gaaactgatg cttcaaatta tatgtatttt tatcatttgg gtagtgaata taaggatgtc
      241 tgacaacagg cattcctgtc ttctttgcct cagcagcaag tgactgcaga taaggtttca
      301 gtgcaagatg gatttttccg aaccgtgcaa agaattttac agtatcatca tctgtatagg
      361 gctgatgatt tcttttcggt ttgtttcctt catgagttct gaaaatagga gtaaatgcgt
      421 tcagttcagt ccagcgcagg aagagttcct tcgatctgtg ataattcatc aaaggattgt
      481 caattgtagt gtaccccccg atatcactgt gattcagagc aattccgctg atccctcctg
      541 aattcagtcc aacaacggtg gaagcaattc cgtcattctc gccgaaactg accatctgat
      601 ctcccagcca gaatgctgtg gagtatttcc ctgaataact gtaccctgct ctagtgaaaa
      661 aaacaatttt tccttctttg cctgcctctg caattgcctc acggttgacc cttgcccagt
      721 ctactggata ctggttatga tagacttccg cagaaattcc agaatgaagt tttgcatcga
      781 aaggaagcca ttctccaaag tctgccatcc agccggcaag accgacgtcg atcatatttt
      841 tctttattat tgactttgtc cacttagcgg cctcaggatt tgtaagatca atcagatagg
      901 caggaaagcc gactgtctga atcagatagt cctttccatc ctggttcttg accagatatc
      961 cttttgcctt tgcttcctca agcataggat tttcaaattt ctcatccgcc tttttcgggt
     1021 ccctgtccgc gagaaatgaa ttgatatatc ccagcacctg tatgtttttc ttgttcatag
     1081 ttttcacaaa ctctgcaaat ttgggataca tctcttcgtc tgcataccat ctccatttca
     1141 gctgatcccc gaatccggtg attcttcttc cgcaccagtc ctgaatccag agcgcagtca
     1201 caggattgcc tgccgccttt gcatcgttca gtatttcatt gatcttatcg tatcctttca
     1261 gctgcttttc acctagcgca ggtgcagtga tttcaccggc ctgaagtccc atccaggtgc
     1321 cgtaagccca gtcaggaagc tcggaatatc tgcctgtttt ttcagtaaac tgtgagatca
     1381 gttcctccgg agtttctccg atccagacag aacccttcag atttccttcc cagaactgca
     1441 cctttactag attctcattt gaaaaatcaa attttgaata agaagagttt tcaaagaata
     1501 cggatcggtt ttttgtactg atgtaaaaag gcattggtgc atatgaagta tattcatttc
     1561 ccccggcgtc ggctgtcaga ttggcaccga atgtaatcgg ctgatcccct cttcctattc
     1621 cctgttcttc tgtaaaaata aaaggagtct ttcctttcat attgaaatga gtatactgtt
     1681 cgccgaatcc gaagaactgc tctgacttgt cactt
//
LOCUS       DAOQGJ010000962         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50856_length_1713_cov_2.768999, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000962 DAOQGJ010000000
VERSION     DAOQGJ010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50856_length_1713_cov_2.768999"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PK683_14070"
     CDS             complement(<1..148)
                     /locus_tag="PK683_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNH09622.1"
                     /translation="MRKAKVAVLGATGSVGQRFIELLENHEFFEVSYLGASDKSAGNT
                     YEEVM"
     gene            351..>1713
                     /locus_tag="PK683_14075"
     CDS             351..>1713
                     /locus_tag="PK683_14075"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09623.1"
                     /translation="MKKLPIGIQTFSKLIENNYLYIDKTRDILNLLEAGSYLFLSRPR
                     RFGKSLLISTLDSIFKGRKELFSGLYIEDKIDWKEYPVIRIDLSTCQTDTVQNLETSL
                     KEVIQVIAETYSVSLQSTLVTGQFKELIRKLSAGSKVVILIDEYDKPIVDYLTNSEML
                     AQMRDILRNFYSVIKGSDEYIHFAFITGVSKFSQVSIFSGLNNIKDISFFPEFASLLG
                     YTQEELEICFSDRFPSLLEKTKMTTTEILSQLRTWYNGYSWDGKNRVYNPYSILNFFD
                     RGEFQNFWFQTGTPTFLIRHIKSEKISIPSMENIEADPEAFESFGINEISFHSLLLQT
                     GYLTVKEIRKEGIAVKYTLDYPNEEVRRSFFFHILNSFLDGTKSTGQISPIVWDMNSA
                     LKNSDTKSFILKLRSLFASIPYTLHMDAEAYYHSLFYMILKLIGCSIDLEVLSSEGRA
                     DGIL"
BASE COUNT          506 a          390 c          322 g          495 t
ORIGIN      
        1 tcattacttc ttcataagta tttccggcac tcttgtctga cgcacctaaa taagaaacct
       61 caaagaattc atgattttcc agaagttcaa tgaacctttg tcctaccgaa ccagttgcac
      121 ccaaaacagc tactttggct tttctcatcc tttctccaga ccagggactg cacgattctg
      181 cacagcccat ttaatttatt tacacagtcc tgtaaatatc cgaactgtgt tcaatatata
      241 acttccctag catatggtct atgtccatat tcaaaaatga agggattcag taaacgaaaa
      301 catttttgaa tttccttgac tgcccggcaa tggcttcgca gactgaaact gtgaaaaagc
      361 tccccatcgg catccagacg ttctccaaac tgatcgaaaa caattacctg tatatagaca
      421 aaacgagaga tattttaaat cttctggaag cgggcagcta ccttttcctt tcacgcccaa
      481 ggcggtttgg caagtccctt ctgatcagca cattggacag tattttcaag ggaagaaagg
      541 aactcttctc cggcttgtat atcgaagaca aaatcgactg gaaagaatat cccgtaattc
      601 ggattgatct ctccacctgt cagactgaca ctgtgcagaa tctggaaact tccttaaaag
      661 aggtcattca agttattgca gagacttatt cagtttctct ccaatccact ctagtaacag
      721 gacagttcaa agagctgatt cgaaaactgt ctgcaggttc taaggtcgtc attctgattg
      781 atgaatatga taagcctata gtggattatc tcacgaattc agagatgctt gctcagatga
      841 gagacatcct gaggaacttt tattctgtaa tcaaaggttc agatgaatac atccattttg
      901 cgttcattac aggcgtatcc aaattttccc aggtttccat attctcaggt ctgaacaata
      961 tcaaggatat atcctttttt cctgaatttg catctctttt gggatacact caagaagaac
     1021 tggaaatctg cttttcagac agatttcctt ctctgctcga aaagacaaaa atgacgacga
     1081 cggagattct ttctcagctg agaacctggt acaacggata cagctgggac ggaaagaaca
     1141 gggtctacaa cccctattcc attctcaact tctttgacag gggggaattt cagaacttct
     1201 ggttccagac tggaactccg acttttctga tcagacacat caaatcggaa aaaatttcca
     1261 tcccctccat ggagaacatt gaggcggatc cggaagcatt tgagtcattt ggaatcaatg
     1321 aaatcagttt ccattccctg cttcttcaga caggatatct gactgttaag gaaatccgaa
     1381 aagagggaat tgccgtaaaa tatacactgg actatccgaa cgaagaagtc agaagatcct
     1441 ttttctttca tatcctgaat tccttcctgg atggaaccaa atcaaccggt cagatcagtc
     1501 caatcgtatg ggatatgaac tcagcactga aaaacagcga taccaagagt tttattctga
     1561 aactccgttc cctgtttgcc tccattccgt atacacttca tatggatgca gaagcctatt
     1621 atcattctct tttttatatg atccttaaac tgatcggatg cagtatagac ctggaagtac
     1681 tgtccagtga aggtcgtgcg gacggcattc tgg
//
LOCUS       DAOQGJ010000963         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_50966_length_1711_cov_2.317633, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000963 DAOQGJ010000000
VERSION     DAOQGJ010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50966_length_1711_cov_2.317633"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PK683_14080"
     CDS             <1..856
                     /locus_tag="PK683_14080"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HNH09624.1"
                     /translation="NIYYNSLISGFNTNDLDKITEEIFLFSGLKDFRKIPLKNYSSGM
                     TMRLAFAMATAGRPDNLLIDEAFAVGDAAFQQSCVERLKTFAGQGSSILIVSHDLSLL
                     SSICSRMILLDKGKLIFSGRTSDTVEKYMELLGRNSGNDNENTLSDIQVSLRSERMIS
                     GEIFFISEILILGIEFSLNSEIDGLTVGFHIENQNGIKIYGTNTHLLKTPPQKNDNLM
                     KKCIFKFPANMNEGKYSVSVSVHTGENHIGSCLYWRERILSFELEKGNHPKFEGTNFL
                     PAEVFWKE"
     gene            complement(857..1492)
                     /locus_tag="PK683_14085"
     CDS             complement(857..1492)
                     /locus_tag="PK683_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNH09625.1"
                     /translation="MPFLKNLKKNNSREWFQDNKKSYEEAKQSFTFITAKLISEISKF
                     DSTVKGIDPESAIFRIYKDVRFSKDKSPYKTNFGAYIAKGGKKSSFPGYYIHIEPNES
                     MAAGGIYMPPPDVLLKLRNFIAENHKDAVKIMKSKKFSEVFPEMYNDRLKTVPKGFSK
                     EHPAAELLKYKSFIFSSMIDDESVDSEKFAEDVLKKYKVIYEWNRFLKKAV"
     gene            complement(1499..>1711)
                     /locus_tag="PK683_14090"
     CDS             complement(1499..>1711)
                     /locus_tag="PK683_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09626.1"
                     /translation="AKGMSYTLYETGNDFSISRMLTYIPDMDEIHIYPKPPVKKLYKP
                     ELCREVQESDFVSLWQKGEERKKKES"
BASE COUNT          512 a          331 c          335 g          533 t
ORIGIN      
        1 aaacatatac tataattcct taatatccgg atttaataca aatgatctgg ataagatcac
       61 ggaagagatt tttttatttt cgggactgaa ggatttcaga aagattcctc tgaaaaatta
      121 ttccagcgga atgacaatgc gcctggcctt tgctatggca actgcaggaa gaccggacaa
      181 ccttctaata gatgaggcat ttgctgtcgg ggatgcagca tttcagcagt cctgcgtgga
      241 aagactgaag acttttgccg gacagggttc ctcgatcctc atcgtaagcc atgacctgtc
      301 tcttttaagt tcaatctgct ccagaatgat cctgcttgac aaggggaagc tgatcttctc
      361 gggaagaaca tcggacaccg tagagaaata tatggagctt ctgggcagaa attccgggaa
      421 tgataatgaa aatacattat cagatattca ggtttctctc agatcggaaa gaatgatctc
      481 aggcgaaatt ttttttattt ctgagattct tatactaggc attgagtttt ctctgaattc
      541 ggaaatcgat ggtctgactg taggatttca tattgaaaac cagaatggga tcaaaattta
      601 cggaacgaat acacatctgc tgaaaacacc tcctcagaag aatgataatt taatgaaaaa
      661 atgcattttt aagtttcctg caaacatgaa tgagggcaaa tactcagtca gtgtatcagt
      721 tcacaccgga gaaaaccata taggaagctg tctttactgg agagaaagaa ttctctcctt
      781 tgaactggaa aaagggaatc atccgaagtt tgaaggcaca aattttcttc cggctgaagt
      841 gttctggaaa gaatgactat actgctttct tgagaaacct gttccattca tagataactt
      901 tatatttttt cagaacatct tcagcaaatt tttcagaatc gactgactca tcatctatca
      961 ttgatgaaaa aataaaactt ttgtatttca gaagctctgc tgcaggatgt tcctttgaaa
     1021 atcccttggg aacggtcttg agacggtcgt tgtacatttc agggaaaacc tcagaaaact
     1081 tttttgactt catgattttc actgcatctt tatggttctc agcaataaaa tttctgagtt
     1141 tcagaagcac atccggaggg ggcatataaa ttccccctgc cgccattgat tcattcggtt
     1201 ctatatgaat atagtagccc ggaaaagagg atttttttcc gcctttggca atatatgcac
     1261 cgaaatttgt cttgtacgga gacttgtcct ttgaaaaacg gacatccttg taaattctga
     1321 aaatcgcaga ttcaggatca atccccttga cagtggaatc aaacttggat atttcagaaa
     1381 taagttttgc cgttatgaat gtgaaagact gctttgcctc ttcataagat ttcttattat
     1441 cctgaaacca ttccctgctg ttgtttttct tcagattttt cagaaacggc aatatcattc
     1501 aggattcctt ttttttcctt tcctcaccct tctgccagag agaaacaaaa tcgctttcct
     1561 ggacttcacg gcagagttca ggcttgtaca gcttctttac aggcggtttt ggatatatat
     1621 gaatttcatc catatccgga atataagtaa gcatacggga tattgaaaaa tcatttcccg
     1681 tttcataaag ggtataagac ataccctttg c
//
LOCUS       DAOQGJ010000964         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51005_length_1710_cov_2.740181, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000964 DAOQGJ010000000
VERSION     DAOQGJ010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51005_length_1710_cov_2.740181"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PK683_14095"
     CDS             <1..878
                     /locus_tag="PK683_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09627.1"
                     /translation="WVMNSVSIAAVETDASYETVHVEIHAWAKDLNISRKLTPEKRAE
                     MLLEKTQEDYFEIWSFVRKRGAVTKYGNGILSGNCPNCGADIRNLGELNQCRHCKSVI
                     NSGEYGWVLSEITQKEEWNPNQKAKTAVPDNIFKYNDTVNRQIIEDRTSYLFWRWMEC
                     LAKGTQKPLSRDASDEMLKNFQPSKKYIGDAAVGAVELLSVSEKTGRYLAKIKILWSA
                     SFSEGAVSQHRRNIFTITIPMGLRKKSGLSENSCESCGAPLPETDALKCSYCGSSLPL
                     IVNDWILESVEKEKI"
     gene            complement(918..>1710)
                     /locus_tag="PK683_14100"
     CDS             complement(918..>1710)
                     /locus_tag="PK683_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNH09628.1"
                     /translation="DTNGLANGLVKYWGGLDGYLRSQGAKVATPGSSAANTIALRSSQ
                     TKSQLDTFMAANGCSKIHLVGHSQGGLVVRYLVKNDGYAGKTATVTTVNSLHKGAPMA
                     NIVLSAIPSWLQPFANSALSLFAKLVYRDGRPQDVIAMGQSLTTNYVQGVLNPATPNV
                     STVKYYSYGSKMAWADLIQHPLMALTHPITWAGGLYYGLGGDNDGVVPLSSQSWGAWK
                     GTPSAKWFATGIDHLQATNFEWSGQTYFDVQGYYLGIAKNAKAGL"
BASE COUNT          536 a          350 c          388 g          436 t
ORIGIN      
        1 aatgggtgat gaattccgta agcattgctg ctgtggaaac agatgccagc tacgagactg
       61 tccatgtgga aattcatgcc tgggcaaaag atctgaatat aagcagaaaa ctgactcccg
      121 aaaaaagagc agaaatgctt ctggagaaaa ctcaggaaga ctatttcgaa atttggtcat
      181 tcgtgagaaa aagaggagca gtcacaaagt acgggaacgg gattctgtcg ggaaactgcc
      241 caaactgcgg agctgatatc cggaacctgg gagaactgaa tcagtgcagg cactgcaagt
      301 cagtcattaa ttcaggagaa tatggatggg ttctttcgga aattacgcaa aaagaagaat
      361 ggaatccaaa tcagaaagcc aaaacagcag ttcctgataa tatattcaaa tataatgata
      421 ctgtaaacag acagatcatc gaagacagaa cttcttatct cttctggcga tggatggaat
      481 gtctggcaaa gggtactcag aaacctttga gcagggatgc ctcagatgaa atgctgaaaa
      541 attttcagcc ttccaaaaaa tatatcggcg atgctgcagt cggtgcagtg gaactgttaa
      601 gcgtgagcga aaaaacagga agatatctgg caaaaatcaa aattctatgg agtgcaagtt
      661 tttcagaggg tgccgtttcc cagcacagaa ggaatatttt tacgattacg ataccgatgg
      721 gactccgaaa aaaatcaggt ctttctgaaa attcctgtga atcctgcggg gcacctcttc
      781 ctgaaacgga tgcattaaag tgcagttact gcggttccag tctgcctttg attgtaaatg
      841 actggatttt ggaatctgta gaaaaagaaa aaatctaaaa aaaagatagc gaggcaggcc
      901 taagcctgcc ttttttatta aagtcctgct ttagcgtttt ttgctatgcc aagataataa
      961 ccttgaacat caaaataagt ctgtccgctc cactcaaaat tagtagcctg taaatgatca
     1021 atccctgttg caaaccattt tgcagaagga gtacctttcc aagctcccca agactgagaa
     1081 gaaagaggaa caacaccgtc attatcaccg cccagaccgt aatagagacc acccgcccaa
     1141 gtaattggat gagtaagcgc catcagtggg tgctggatta aatcagccca agccattttg
     1201 cttccatagg aatagtactt cactgtagaa acattaggag tagcaggatt caggacacct
     1261 tgtacataat ttgtagtgag agactgaccc atagcaatta catcctgtgg acgcccgtca
     1321 cgatatacaa gtttagcaaa aaggctgaga gcactgtttg caaatggctg aagccagctg
     1381 ggaattgcac tcaggactat attagccata ggtgcacctt tatggagtga gttgactgtg
     1441 gtaactgttg ctgttttgcc tgcatatccg tcattcttta ccagataacg aactacaagt
     1501 cctccctgag aatgtcctac cagatgaatt ttagaacagc cgtttgctgc cataaaagta
     1561 tccagttggg attttgtctg tgaagatctg agagcaatag tatttgctgc tgaactgccc
     1621 ggtgtagcaa cttttgcacc ttgacttctc aggtatccgt ctagacctcc ccaatatttt
     1681 acaagtccgt tagccagtcc gtttgtatcg
//
LOCUS       DAOQGJ010000965         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51040_length_1709_cov_3.298670, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000965 DAOQGJ010000000
VERSION     DAOQGJ010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51040_length_1709_cov_3.298670"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PK683_14105"
     CDS             <1..630
                     /locus_tag="PK683_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09629.1"
                     /translation="LSWLSENSFEKLLVRFIENGFSADKKLFIELKLSPKIFHPKKRS
                     FFPDSVSTSEKKLIEEIFSVLGRIISVYPDRKEEIRKSVGFISFSLAALHSAYALSEE
                     IYEYHLITTTDQFLKKSLSRALFYIPLTSEEKTKIICSEWLTGIWFDPRYITDPVRTF
                     LEINEQRRNRLKFYVSSYGMEFSKLPDRFIRENGEKLPLKGIIFDINDE"
     gene            707..1456
                     /locus_tag="PK683_14110"
     CDS             707..1456
                     /locus_tag="PK683_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH09630.1"
                     /translation="MKAFYYIFSVFYWVGIVGLTSVLFFPVAAVIKGLTFLFDKNLKI
                     LHMFTSFWGSIYTWVNPLWNVKTEGREKIRKDGVYVAVSNHFSTTDVFTVYRLFFHFK
                     WVSKSENFKIPLIGWNMTLNRYIRLNRGSTKGNAQMMKDAEAALRQGSSVYIFPEGTR
                     STTNEVKEFKTGAFELAKRTKSPILPIVIEGSQNALPKKGVILNGRHNILIRVLDEIP
                     YTEFEHLESAEIAEKVRALIAGELSKIRHPG"
BASE COUNT          550 a          339 c          343 g          477 t
ORIGIN      
        1 ctttcctggc tttccgaaaa cagctttgaa aaactgctgg tcagattcat tgaaaacgga
       61 ttcagcgctg ataaaaaact cttcattgaa ctgaaacttt cccccaagat ttttcatccc
      121 aaaaaaagaa gtttctttcc ggattcagtc agcacctctg aaaaaaagct gatagaagaa
      181 atcttttctg ttctgggcag aatcatttca gtataccctg acagaaagga agaaatcaga
      241 aaatcagtag gattcatttc cttcagtctg gcggctctcc actcggccta tgcactctcc
      301 gaagaaattt atgaatatca cctgatcacg acaacagatc agttcctgaa aaaaagtctt
      361 tccagagcat tattctatat ccctttaacc tcggaagaaa agacaaaaat aatctgttct
      421 gagtggctta ccggaatctg gtttgatccg agatacatca ctgatcctgt ccggactttc
      481 ctggaaatca atgaacagag aagaaacaga cttaagtttt atgtatcttc atacggaatg
      541 gagttttcca agcttccgga cagattcatc agagaaaacg gagaaaaact tcctcttaaa
      601 ggaatcatct ttgatatcaa tgacgagtga acggatcggt tttctcagaa taaaaaagtt
      661 gacttctgca gttccgttga aatactcttg tctggataag gatttcatga aagcattcta
      721 ttatattttt tccgtatttt actgggtcgg cattgtaggc ctcacatctg tcttattttt
      781 tccagtcgca gccgtaataa aaggacttac atttcttttc gataaaaacc tgaaaattct
      841 gcatatgttc acatcttttt ggggaagtat ttatacctgg gtcaatcctc tctggaacgt
      901 aaagacagaa ggcagggaaa aaattcggaa agacggagta tatgtcgctg tatcaaatca
      961 tttttccact acagatgtat ttacagtgta caggcttttc tttcatttta aatgggtttc
     1021 caagtcggaa aatttcaaga ttcctcttat tggatggaat atgacattga acaggtatat
     1081 ccgtctgaac agaggaagca caaagggaaa tgcacagatg atgaaagatg cggaagcggc
     1141 tctcaggcag ggcagctctg tatatatttt tcctgaaggc acaagatcaa cgacaaatga
     1201 agtaaaggaa tttaagacag gggcatttga acttgcaaaa agaaccaaaa gtcccattct
     1261 ccccattgtc atcgaaggaa gtcagaatgc tcttcccaag aaaggggtga tactgaacgg
     1321 acgtcacaat atactcatcc gggtgctgga cgaaattccg tacacagaat ttgagcatct
     1381 ggaaagtgca gagattgcag aaaaagtaag agcactgatt gccggcgagc tgagtaaaat
     1441 ccgtcatccc ggataaaaaa tatactgtca ttattccgat ttatactcgg gctgggcgtt
     1501 atataaacgc tcagattgtg tgaataggat attttataat gcgcagagag tcctagcagt
     1561 ctgcaaaact tcaaaaaata taatgctctg caggcaaatg tttaagcctg cctccgaaag
     1621 aatatccctc cgttaataaa tgtctccgct gctgaagata aaagctgaat agattcattt
     1681 tgcagccgca taaaagcaag gctgcttac
//
LOCUS       DAOQGJ010000966         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51184_length_1706_cov_3.588734, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000966 DAOQGJ010000000
VERSION     DAOQGJ010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51184_length_1706_cov_3.588734"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /gene="guaA"
                     /locus_tag="PK683_14115"
     CDS             complement(<1..763)
                     /gene="guaA"
                     /locus_tag="PK683_14115"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004470732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNH09631.1"
                     /translation="MEFTKKIGVIDFGGQYAHLIASRLRRLGAYTEIISNEETLNKYR
                     EFSGLVFSGGPASVYEENSPQISADVLNLNIPILGICYGHQLIMKLLGGEVYKSAKSE
                     YGLAYLERVEPSKLTEGFENSEQVWMSHGDEVVKIPEGFSVFARSSHCSFAGVFSEEK
                     KIYCVQFHPEVTHSVKGNILLENFIRITGSSGTWNLAEFLKLKTEEILVSIPAGKKVF
                     MLVSGGVDSTVAYTLLTRILGKDRVAGLLVDTGFMR"
     gene            929..1528
                     /gene="msrA"
                     /locus_tag="PK683_14120"
     CDS             929..1528
                     /gene="msrA"
                     /locus_tag="PK683_14120"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNH09632.1"
                     /translation="MKYFIYLMSLFLLSNSVFAQKKNEPVQSKTGTAIFAGGCFWCME
                     PPFEKLDGVISVISGYTGGTKKNPTYEEVSAGGTGHTEAVKIEFDPKKISFKKLLDVF
                     WRNIDPTVKDRQFCDSGPQYRSGIFYLNDEQKKEALESRKGLEKKFSSIYTEITPIGD
                     FYPAEEYHQDYYKKNPIRYKYYRHGCGRDKRLEELWGKN"
BASE COUNT          530 a          353 c          350 g          473 t
ORIGIN      
        1 ttctcatgaa tccagtatcc accagaagtc cggccactcg gtcttttccc aggattcttg
       61 tcagaagagt atatgcaact gtggaatcca ctcctccgga tacgagcata aacactttct
      121 tgcctgcagg tatggaaacc agtatttcct ctgtcttcag ctttagaaat tcagccagat
      181 tccatgtccc ggaagagccg gtgatcctga tgaagttttc cagaagaatg tttcctttta
      241 ccgaatgagt cacctcggga tgaaactgta cacaatatat ctttttttcc tcagaaaata
      301 caccggcaaa actgcagtga gaacttctgg caaatacgga aaatccttcc ggaattttaa
      361 ccacctcgtc gccgtgactc atccagacct gctccgagtt ttcaaagcct tccgtaagtt
      421 ttgagggctc cactctttca aggtaggcaa gcccatattc gctttttgcg gatttgtata
      481 cttcccctcc caaaagtttc ataatcagct ggtgaccata gcagatgccc aaaatcggaa
      541 tgttcagatt cagtacatca gcagatattt gaggtgaatt ttcttcatag acgcttgcag
      601 gccctccgga aaaaacaagt cctgaaaatt ctctgtattt attcaaagtc tcttcgtttg
      661 aaatgatttc agtgtatgca ccgagtcttc tgagtctgga agcaatcaaa tgtgcatatt
      721 gcccgccaaa atcaattacg ccgattttct tcgtaaattc catagctaat acaaaacttt
      781 atcagcttgg atatttggac agaaaaattc tgtcgaagaa tatcgtggaa tagacgttgg
      841 agaaactgga tttccattac agccgatgag cctaaaaaag gatgtaactt ttcagacaga
      901 tattcgaaag aatattcagg agatcccaat gaaatatttt atctatctga tgtctttgtt
      961 cctgctttca aattcggttt ttgcacagaa aaagaatgag cctgttcagt ccaaaacagg
     1021 tactgcgata tttgcaggag gatgtttctg gtgtatggaa cccccattcg aaaaactgga
     1081 cggtgtcatc agtgtcattt ccggctacac agggggaaca aaaaaaaatc cgacctatga
     1141 agaagtttca gcggggggaa caggccatac tgaagctgtg aagatcgaat ttgatccaaa
     1201 aaaaatcagt ttcaaaaaac ttctggatgt attctggaga aatatcgatc ccactgtaaa
     1261 agacaggcag ttctgtgatt caggacctca gtatagatca ggaatatttt acctgaatga
     1321 cgaacagaaa aaggaggcac tggaatccag aaaaggactt gaaaaaaagt tttcttcaat
     1381 ttacactgaa atcacaccca tcggagattt ttatcctgca gaagaatatc atcaggatta
     1441 ttataaaaag aatccgatcc gatataaata ttacagacac ggatgcggaa gagacaaaag
     1501 actggaagaa ctctggggaa aaaactagtc tgcagctgaa gatgaatctg atcgaaactg
     1561 cttttaactt tccgaaccct gttcattaca ttacacggta attgaattta catagaggct
     1621 ggaaagttga gtgttgacaa acggctggca ctgtgcaggg tgccgcagtt tggaaaactg
     1681 cagttttgaa tctttctttg aacagg
//
LOCUS       DAOQGJ010000967         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51306_length_1704_cov_3.360218, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000967 DAOQGJ010000000
VERSION     DAOQGJ010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51306_length_1704_cov_3.360218"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PK683_14125"
     CDS             <1..1112
                     /locus_tag="PK683_14125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH09633.1"
                     /translation="LEQRVERRKTIFVLSEAEKYKYHKISLFNNQSVTMPDAFVKETG
                     MDSDENAPVWSEKGANEISMRVSSAAFLYSSAEKNPDSMYSKAPEQFSVLTFNYPGGR
                     KKSWWATEDEAMFYNYLKKNNALKLDVMWGPSQPEIQTEMFKFAQIKSSDIVYDLGCG
                     DGRVLVQAAKQFGAKGIGYDLDPDKIAAAEKLAKANKVENLVSFKNANMFDQQIGEAT
                     VITLYLNISVNARLRPKLFQELQPGTRIVSHNWHMGEWKADGVKSVGKRIVFYWLLPA
                     DFSGEWTGSTKNFGDIKVNLTQKFQMVTGEVSLNGKSYAVKDSKVKGKTLALNLEKGL
                     DITLHSDSEILSITSQGKDSSEKITRTSKGSGKIY"
BASE COUNT          575 a          310 c          353 g          466 t
ORIGIN      
        1 cattggaaca gagagtagaa agaaggaaaa cgatctttgt actttctgag gcagaaaaat
       61 ataagtacca taaaatcagt ctttttaata atcagtcagt gactatgcct gatgctttcg
      121 taaaagaaac cggaatggat tctgatgaaa atgcgcctgt atggtcggaa aaaggcgcca
      181 atgaaatcag tatgagagtt tcaagtgcag cttttttata ctcttcggct gaaaaaaacc
      241 ctgactcgat gtacagtaaa gcccctgagc agttttctgt tttgaccttc aattatccgg
      301 gcggcaggaa gaaaagctgg tgggctactg aagatgaagc gatgttttac aattatctga
      361 aaaagaacaa tgctctcaag ctggatgtta tgtgggggcc gtcccagcct gagattcaga
      421 cagagatgtt caagtttgct cagatcaaat ccagtgacat cgtatatgat ttaggatgcg
      481 gtgacgggcg agttcttgta caggcggcaa agcagtttgg agcaaaggga atcggatatg
      541 atctggatcc ggacaaaatc gctgcggcgg aaaaactggc aaaggcaaac aaagtggaaa
      601 accttgtaag tttcaagaat gcaaatatgt ttgatcagca gatcggtgaa gcaactgtaa
      661 ttacattata tttaaacatt tcagtaaatg cccggcttcg tccaaaactt tttcaggaac
      721 ttcagcctgg aacaagaatt gtttcccaca actggcatat gggagaatgg aaggcggacg
      781 gtgtaaaatc agtagggaag agaattgtat tctactggct tctcccagcg gatttcagcg
      841 gcgagtggac aggttcaacc aagaatttcg gtgacatcaa agtgaatttg actcagaaat
      901 ttcagatggt gacaggtgaa gtcagcctga atggaaaatc ctatgcagtc aaagattcaa
      961 aagtgaaagg aaagacgctt gctctgaatc tggaaaaagg tctggatatt acacttcact
     1021 ccgattctga aattctcagc ataacatctc agggcaagga ttcatcagaa aagattacaa
     1081 ggacatctaa gggttcagga aaaatttact gaaaacaatt cgagaaagcg agtctttaac
     1141 tcgctttcat tcaatacttt tctttaagtc tttcctgcct gatcttctca agccgactgc
     1201 cgcatatttc agatacattt ttttccttaa aaataaatac aaaaacagct gattcttccc
     1261 atagttccag aatctcagaa aaatcaccgc tgatgctgta aatttgcagc aaatacagaa
     1321 accgtcaaaa ttcatcagac agaaagaact gaatgcagga ataaactgtt tttgaaaaag
     1381 aaagtatctg aacagcagac ttgccttatc ggttaaatcc tggaaaatat gtattttttt
     1441 cagaatattg ccgggaaaca ttcaagaata tgatttttat catatttcca tgagtattcc
     1501 gaacattttc tcgaagaaat ctgccgcatt ttctgataaa aaataatgat aaaaataaat
     1561 acagaatata aaacatataa tttttcagcc aataaaatca agcatttcca agtaattatt
     1621 tcctttccgg catcagtttt cctgactgga ctgcattgtt tatcacttgt tcagaaagaa
     1681 gctcttcaac agtacatgaa cagg
//
LOCUS       DAOQGJ010000968         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51502_length_1700_cov_3.655319, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000968 DAOQGJ010000000
VERSION     DAOQGJ010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51502_length_1700_cov_3.655319"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PK683_14130"
     CDS             complement(<1..1306)
                     /locus_tag="PK683_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNH09634.1"
                     /translation="MFSPFNTEEMMPVRVTKEHRTRLIIFLGAFAVLLSVLLGRVIYL
                     TFLNKKISEIKKEVRVHRGMIFDRRGLELALSQDSTTIGIDPANVYEPAFTANKLSPY
                     VKIPSSRIESMILEKDRYFLLKRELDDDNAQKIGDMHLPGVRMEKEYKRIYPNGTLAS
                     SVIGFTGMDDNKALAGIELMFNDELMRRTEDDSPRGSDIYLSIDTLMQYKLETALGKA
                     FEETRSKKAVGIFMDVTNGKIIAMASFPNFDPNRYYDYPPEATANWAIRHIYEPGSTM
                     KIFIAMVLMNEKKVGPNEKFYCPGYVEFGERIVNCTEQHGLVDLEEILQYSCNVGIIK
                     AISKLPDDVLFKYLDKMKFGQKTGFSNYEQKGFFPGLKQWNPSTSYYLSIGQGIEVTP
                     LQLVTAASALVNGGKVHTPVIVSHITDIYGEITKRYTSNPVQL"
     gene            complement(1290..>1700)
                     /gene="lepB"
                     /locus_tag="PK683_14135"
     CDS             complement(1290..>1700)
                     /gene="lepB"
                     /locus_tag="PK683_14135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNH09635.1"
                     /translation="KYKFPQKLSSAKEFTFFGRLKVNRLDQVLFEAPNGDYYVKRIIG
                     LPGDFYAFRGMEVLIDSKPLNETYILRPENFPILTPKDMQTGNFYNLLSEGRIPPGYY
                     LLLGDNRKISYDSRDLGLIPEERLRGKVVYVFSL"
BASE COUNT          452 a          388 c          341 g          519 t
ORIGIN      
        1 caagctgcac aggattggaa gtgtatcttt ttgtaatctc cccgtaaata tcagtgatat
       61 gagacacgat cacaggtgta tgaacctttc cgccgttgac cagggctgat gcagctgtga
      121 caagctgaag aggagtcact tcaatcccct gcccgatcga aaggtagtag gaagtactcg
      181 ggttccactg cttcagtcca ggaaaaaatc ccttctgttc ataattggaa aatccagtct
      241 tctgtccgaa tttcatttta tccaggtact tgaaaagaac atcgtccggg agtttgctga
      301 tcgccttgat gattccgaca ttgcaggaat actgaagaat ttcttccaga tccacaagac
      361 cgtgctgttc agtgcagttg acaatcctct ctccgaattc cacataaccg gggcagtaaa
      421 acttttcatt cggaccgact ttcttttcat tcatcagaac cattgcaatg aatatcttca
      481 ttgtggaacc cggctcgtat atatgacgta ttgcccagtt tgcagtcgct tcaggcgggt
      541 agtcataata tctgttcgga tcaaaattcg gaaaacttgc cattgcaatg atctttccgt
      601 ttgtcacatc catgaatatt ccgacagcct ttttggatct ggtttcttca aatgcctttc
      661 caagtgcagt ctccagcttg tactgcatca gtgtatcaat ggaaagataa atatccgatc
      721 cgcgaggaga atcatcttct gttcttctca tcagttcatc attgaacatc agctctattc
      781 ccgccagagc cttattgtcg tccataccgg taaacccgat tactgaagat gcaagagttc
      841 cattaggata tatccttttg tattcttttt ccattctcac gccgggaaga tgcatatcgc
      901 ctatcttctg cgcattatca tcgtccagtt ctcttttcag aagaaagtat ctgtcctttt
      961 ccagaatcat ggattcaatt ctcgaagaag gaatttttac ataaggagaa agtttgttcg
     1021 cagtaaatgc aggttcatag acatttgcag gatcaattcc gattgtagtg gaatcctgcg
     1081 aaagtgcaag ttccagtcct ctcctgtcga agatcattcc tctgtgaact ctgacttctt
     1141 ttttgatttc tgagattttt ttattcagga aagtaagata tatgactctt cccaaaagca
     1201 cagaaagcag tacagcaaag gcccccagga aaataataag tcttgtcctg tgttccttgg
     1261 tcacgcggac aggcatcatt tcttcggtat taaagggaga aaacatatac cacttttcct
     1321 ctcagtctct cctccggaat cagccccaga tctctggaat cataggaaat ctttctgttg
     1381 tcgccgagca gaagatagta acccggcggg attcttcctt ctgaaagaag attgtagaag
     1441 tttcctgtct gcatatcctt aggggtcagt attggaaaat tttccggtct gagaatatac
     1501 gtttcattca gaggcttgga atcaatcaga acttccattc cgcgaaatgc ataaaaatct
     1561 ccaggaaggc caatgattct tttcacataa taatcgccgt tcggagcttc aaacagaacc
     1621 tgatcgagtc tgtttacttt cagcctgccg aaaaaagtga actcttttgc cgagcttagt
     1681 ttttggggga acttgtactt
//
LOCUS       DAOQGJ010000969         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51516_length_1700_cov_2.894225, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000969 DAOQGJ010000000
VERSION     DAOQGJ010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51516_length_1700_cov_2.894225"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..793)
                     /locus_tag="PK683_14140"
     CDS             complement(92..793)
                     /locus_tag="PK683_14140"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNH09636.1"
                     /translation="MIRNILINLLILTFFWKGRIRDEDFIIHIADPKKGNLEMHWKDS
                     SGKPHRNFRSVKKELEKKGQKLIFAMNGGMYEEDRSPVGLYIQDGRELNRINLKKGKG
                     NFYTMPNGVFSVLKDGTADISVSDKIPSLSKIRFATQSGPMLLVGGEIPKKYSKNSAL
                     RKIRNGVGLLSDGKVIFAVNKKELNIHEFALFFKEKGCKEALYLDGTISSVYLPEKGE
                     DFLGGDFGVIITERE"
     gene            complement(860..>1700)
                     /locus_tag="PK683_14145"
     CDS             complement(860..>1700)
                     /locus_tag="PK683_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HNH09637.1"
                     /translation="LKNYSEAERIKINSLIYQTLKKNVPYRFLAPFLRNSILETEGAF
                     EKQIVKLSNEQFGKELKPIYRLYSDCIELDADWAAYFNENFTIIKGFIFWHLLKFLQR
                     RNENVSGISEKLFKPESRDLKTAKIFWTEYLKKKKEIQCIYSGERVSLDNISLDHFIP
                     WKYTVHDRLWNIIPTTVNMNSRKSDRLPVIETFFQPFADIQYDALYTVLENDSSEIRK
                     LTSDYSDLFLKEDLVKDRIEKNQFTEKLKDTIVPMRITAHGMGFEDLDTVKDSYEIRK
                     RFQ"
BASE COUNT          452 a          380 c          293 g          575 t
ORIGIN      
        1 cttgccggat ttttggcagt gtccaagtcg ccgagccagc ttccatacag gcggccctct
       61 tcataaagct ttggggccct ggctcagata ttcactccct ttcagtgatg atgaccccga
      121 aatctccccc gagaaaatct tcgccttttt caggtagata gacggaagaa atggttccgt
      181 ccagataaag tgcctccttg catccttttt ctttaaaaaa taatgcaaac tcatgaatat
      241 ttaattcttt tttgttcact gcaaaaataa ctttcccgtc agaaagaagc cctactccgt
      301 tcctgatctt ccgaagggca gagtttttgc tgtatttttt cgggatctcg cctccgacca
      361 gaagcatcgg tccggactga gtcgcaaacc tgatttttga aagactagga attttatccg
      421 atacagaaat atctgcagtg ccgtctttca aaacggaaaa tactccgttc ggcattgtat
      481 aaaaattccc ctttcccttt ttcagattaa ttctgttcag ctcacggccg tcctgaatgt
      541 acagtcctac aggactcctg tcctcctcat acattccgcc gttcattgcg aaaatcagtt
      601 tctgcccttt cttctccagc tcctttttta cgcttctgaa atttctgtgg ggctttccgg
      661 aactgtcctt ccagtgcatt tccagatttc cttttttcgg atcggcaata tgaatgatga
      721 aatcctcatc cctgatcctt cctttccaga agaatgtcag aataagcaga tttattaaaa
      781 tgtttctgat cacaggatta cccgactgac tgaaatttaa actgagaaag gaatctgtcc
      841 ggcagaaata agttcagtgt cattgaaatc ttttcctgat ttcataggaa tcctttacag
      901 tatccaggtc ttcgaacccc attccgtgag ctgtaattct cattggaaca attgtatctt
      961 tcagcttttc agtaaactga ttcttttcaa tccggtcttt caccagatct tctttcaaaa
     1021 acagatcgct gtaatccgat gtgagttttc tgatttctga actgtcattt tcaagaacag
     1081 tatacaatgc atcatattga atatcagcaa atggctgaaa gaatgtttca attacaggaa
     1141 gtctgtcact ttttcgggaa ttcatattta ccgttgtagg aatgatgttc cagagcctgt
     1201 cgtgaacagt atatttccaa gggatgaaat gatccagaga aatattgtcc aagctcactc
     1261 tttctccgga atagatgcac tgaatttcct ttttcttttt caaatattct gtccagaaaa
     1321 tttttgcagt ttttaagtct cttgattccg gcttgaagag cttttcagaa ataccggata
     1381 cattttcatt acgcctctgc agaaacttca aaagatgcca aaatataaat ccttttatta
     1441 ttgtgaaatt ttcattaaag tatgcggccc aatccgcatc caattcaata cagtctgaat
     1501 acagcctgta tatcggtttc agttctttcc cgaattgttc attggaaagt tttacgatct
     1561 gtttttcaaa agctccctct gtttccaaaa tggaatttct cagaaacgga gcaagaaatc
     1621 tgtaggggac atttttcttc aatgtctgat aaattaatga atttattttt attctttctg
     1681 cttcactgta atttttcagc
//
LOCUS       DAOQGJ010000970         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51519_length_1700_cov_2.632219, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000970 DAOQGJ010000000
VERSION     DAOQGJ010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51519_length_1700_cov_2.632219"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PK683_14150"
     CDS             <1..528
                     /locus_tag="PK683_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNH09638.1"
                     /translation="KKITIVLGNILPFYYIAEKLKLNAQEKETLTRLMYSKDTEEIRN
                     FLSGKKGSRDIFDILHHLLFSYSFREILPALLILEGKYGFPLKNCIKETEELLKFSEN
                     MKSIEICPDFSLVRDMDYYTGFVFQGYAGRLSFPLVQGGAYDRLFQKFTGEEKNACGF
                     SLNIDIIEELIREEQ"
     gene            530..>1700
                     /locus_tag="PK683_14155"
     CDS             530..>1700
                     /locus_tag="PK683_14155"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017854215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNH09639.1"
                     /translation="MPAELVVGAQWGDEGKAKVIDYLSKDTDIIVRYQGGANAGHTVV
                     VGKNKYVFHLVPSGVIYPKTQCVIGNGVVLDPEFFLEECDHLTKEGFDVYKKTFISDA
                     CHLLLPFHRTIDSLIESSSEPGKKIGTTKRGIGVCYADKMMRVGLRAGDILEDDTLED
                     RLKHAVDLKNKTLNKLYGETPASYKEILDQLKHFRDKAGHMIINTAYYLNEQLKQGKF
                     VLLEGAQGTGLDVDFGTYPYVTSSNSTTGGAISGTGISYHYLGKVYGISKAYATRVGE
                     GPFPTELFGEEGDRMRKLGSEFGATTGRPRRCGWFDTEMIKHAIRVNGMSTLVLTKID
                     ILSDYDKIPVGVGYEINGKKLNFLPSHGMEKVKVIYEELPGWKSDITGINEFSKLP"
BASE COUNT          533 a          301 c          418 g          448 t
ORIGIN      
        1 aagaagatta cgattgttct cggcaatatc cttccctttt attatattgc tgaaaagctg
       61 aaactgaacg ctcaggaaaa ggaaactctg acaagactga tgtattccaa ggatactgaa
      121 gaaatcagaa atttcctttc cggaaaaaaa ggaagcaggg acatctttga catactgcat
      181 catctgcttt tcagttattc attcagggaa attcttcctg cacttctgat tctcgaaggg
      241 aaatatggat ttcctctgaa gaactgcatc aaggaaacag aagaactctt aaaattttcg
      301 gagaatatga agagcattga gatctgtccg gatttttcat tagtaagaga tatggattat
      361 tatacaggat tcgtttttca gggatatgca ggcagactgt ctttccctct tgtgcaggga
      421 ggggcatatg acaggctctt tcagaaattc acaggtgagg aaaaaaatgc gtgcggattt
      481 tcactgaaca tagacattat agaagaatta atcagagagg aacagtaata tgccggcaga
      541 actggtggta ggcgctcagt ggggcgatga aggaaaagct aaagtcattg attatctaag
      601 caaggatact gatataatcg tccggtatca gggcggtgca aatgcgggac atactgtggt
      661 tgtcggcaaa aacaaatatg tatttcatct tgttccttcc ggtgtaatct atccgaaaac
      721 tcagtgtgtc atcggcaacg gggttgttct tgatcctgag ttttttctgg aagaatgtga
      781 tcatctgaca aaggaagggt ttgacgtata taaaaaaact tttatcagtg atgcatgtca
      841 tctgctgctt ccttttcaca gaacaattga ttctctgata gaaagcagtt ctgagcctgg
      901 taaaaaaatc ggaacgacca aaagaggcat cggggtctgt tacgcggata aaatgatgag
      961 ggttggtctg cgtgcaggcg acatactgga agacgatact cttgaagaca gactcaaaca
     1021 tgcagtcgat ctgaagaaca agacactgaa caagctgtat ggagagactc cggcatccta
     1081 taaggaaatt cttgatcagc tcaagcattt cagagacaaa gcagggcata tgattatcaa
     1141 tactgcttat tatctgaatg agcagctgaa gcaggggaaa tttgttcttc ttgagggagc
     1201 acagggaaca ggactggatg ttgattttgg aacctatccg tatgttacaa gttcgaattc
     1261 aactacaggg ggagccattt caggcactgg gataagttat cattatctgg gaaaagttta
     1321 cggaatcagc aaggcttatg cgaccagagt aggcgagggc ccttttccga cagaactctt
     1381 cggagaagag ggagacagga tgagaaaact tgggtctgaa ttcggtgcaa ctacaggaag
     1441 accgagaaga tgcggatggt ttgatactga aatgatcaaa catgcgatcc gtgtaaacgg
     1501 aatgtccacc cttgtgctga cgaagataga tatcctttct gattatgata aaattccggt
     1561 gggcgtagga tacgaaatca acgggaaaaa actgaatttt ctcccttctc acggaatgga
     1621 aaaagtgaaa gtgatttatg aagaactccc gggctggaag tcggacatta caggaatcaa
     1681 tgaattttcc aagcttcctg
//
LOCUS       DAOQGJ010000971         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51586_length_1699_cov_1.966545, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000971 DAOQGJ010000000
VERSION     DAOQGJ010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51586_length_1699_cov_1.966545"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PK683_14160"
     CDS             <1..532
                     /locus_tag="PK683_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNH09640.1"
                     /translation="LFDQVAAKAEAQFKAAGTSYKYFLIDDAVAQIMKNEGGMLWALM
                     NYDGDVQSDMVASGFGSLGLMTSVLVSPEGKYEYEAAHGTVTRHYRQYQKGETTSTNS
                     VASIFAWTGALHKRGELDGNKDLQNFAERLEKAVIDTIESGEMTKDLIPLSNAPAKKQ
                     LDTFQFMEAVQKRLDK"
     gene            635..>1699
                     /locus_tag="PK683_14165"
     CDS             635..>1699
                     /locus_tag="PK683_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09641.1"
                     /translation="MKFFHYSILILAYGCATAGNMQLGEIPKSEEALKSSLEKIGSIP
                     ELSAVTKDFCSNETSLSGIGNYIKFKIADKSWEEVQGELIKTNTPESIEESKNLQKAR
                     AVIASCAKELYGAVPTGSARNLAEIDTKNALKDIDMSVPLENVESSNIWLKKIVPDPD
                     RMMLVTLLGDPIAALPCKEQKGKHALTCARANALETAHILLINEIRNSAHKDSLSAPM
                     SKLANFCPSGFILPPEKSFVYSFFEAAGPDEEKKFTEGLPASPTKESKYRSSTRYRIL
                     FNSVKNPAYYMQAGSIKRWVIDREKDFSEKLAKDFPDYVSNRPVQETEREGALFEQMI
                     HSLRHEESFYYRWKYFFRAVD"
BASE COUNT          564 a          341 c          372 g          422 t
ORIGIN      
        1 tctttttgat caagtggcag cgaaggcaga agctcaattc aaagccgcag gtaccagcta
       61 caagtacttc ctgattgacg atgctgtagc gcaaatcatg aaaaatgaag gcggaatgct
      121 ctgggcactg atgaattatg acggcgacgt tcagagtgat atggttgcat ccggattcgg
      181 ttcacttgga ctgatgactt ctgtgcttgt ttctcctgaa ggaaagtatg aatatgaagc
      241 tgctcacgga acagtgacaa gacattacag acagtatcag aagggagaaa ccacttctac
      301 aaattctgtg gcatctatat ttgcctggac aggtgctctt cataaaagag gggaactgga
      361 cggaaacaag gatcttcaga atttcgcaga aagactggag aaagctgtca ttgacacgat
      421 tgagtccggc gaaatgacta aagacttgat cccattgagt aatgctcctg ccaaaaagca
      481 gctggataca ttccagttta tggaagcagt tcaaaaaaga ctggacaagt aacagtaaaa
      541 aaaccgcatc tcatttgaga gactgcattg cagtctctca aagaatttat ctgttcgaaa
      601 ataaaaacca aaacagcaaa acaatggggc tactatgaaa ttttttcatt attcaattct
      661 gatattggca tatggatgcg caacagcagg aaatatgcag ttgggtgaga tcccgaaatc
      721 cgaggaagca ttaaagtcct ctctggaaaa aattggcagt atccctgagc tgagtgcagt
      781 cacaaaggat ttctgctcaa atgaaacgtc tctttcaggg ataggaaatt atattaaatt
      841 caagatagcc gacaaaagct gggaagaagt tcagggtgaa ctgatcaaga caaatacgcc
      901 ggaatcaatt gaagaaagca aaaatcttca gaaagccaga gcagtcattg catcctgtgc
      961 aaaagaactt tacggagcag ttcctaccgg atctgcgaga aaccttgctg aaattgatac
     1021 aaaaaatgcg ttgaaggata ttgatatgag cgttccgctc gaaaatgtgg aatcctccaa
     1081 tatatggctg aaaaaaattg ttcctgatcc ggacagaatg atgcttgtaa cacttttagg
     1141 agatccgatt gcagccttgc cctgtaagga acagaaaggc aaacatgctc taacctgtgc
     1201 ccgtgcaaat gcactggaaa ctgcacatat acttctcatt aatgagatca gaaactccgc
     1261 tcataaagac tccctttctg cgccaatgtc aaagcttgcc aatttctgcc cttctggatt
     1321 catccttcca ccggaaaaat cttttgtata cagtttcttt gaagcagccg gtcctgatga
     1381 agaaaaaaaa ttcacagaag gacttcctgc gtcccccacc aaggaatcca aatacagaag
     1441 ctcaactagg tacagaattc tgtttaattc agtgaagaat cctgcgtact atatgcaggc
     1501 aggttccatc aaaagatggg tcatagacag agaaaaagac ttctccgaaa aactggcaaa
     1561 ggactttcct gactatgtat ccaacagacc ggttcaggaa acagaaagag agggagcact
     1621 ttttgaacag atgattcatt ctctccgtca tgaagaaagt ttttattaca gatggaaata
     1681 ttttttcaga gcagttgat
//
LOCUS       DAOQGJ010000972         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51607_length_1698_cov_3.614729, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000972 DAOQGJ010000000
VERSION     DAOQGJ010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51607_length_1698_cov_3.614729"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1698
                     /locus_tag="PK683_14170"
     CDS             19..>1698
                     /locus_tag="PK683_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNH09642.1"
                     /translation="MQRFYTELNPSINYTRNNLRWDAWGAYTEDFFLKDHINEILNIL
                     RNDWGVSSLPRTPGVKMDDLKVPKSRLDKNLLAGLSKILPVKTDLQERVFHSAGRSYY
                     DVIRLRFNLLKDYVDGVVYPKTEEEIRSLLLFCKTKKIAVVPFGGGSSVVGGVEARKG
                     KQHKAVISVDMTDMKELVDIDPVSCTASFQAGIYGPVLEHILNERGFTLGHFPQSFEY
                     STLGGWIAARSSGQQSGKYGRIDKIINSARIITPNGTIETWKVPLSSAGPDMNQIISG
                     SEGLLGIITQVTLKIQPLPKSRKYFGIVFPDFASGVGFIREANRKGLKLSMMRLSDKI
                     ETNQYSRFGTIGKPMTAFRRFKYAMIEKILQWNDILDNRCALIGGVDSDEIEVERQIH
                     EAKQTAKHHSGFFAGEGPGKNWLKSRYNMPFLRNHLLENGIGVDTLETVITYDRILTL
                     HKHVEEAIKNTMQCTVMCHVSHSYADGASLYFTIIFPLDLKDPCGQWIKMKKAASDAI
                     YSSGGSISHHHGVGTDHQEWYLKQAGETAVTALKGLKNSLDKEGIMNPGKLF"
BASE COUNT          514 a          335 c          416 g          433 t
ORIGIN      
        1 atcagataag gaatgaatat gcagagattt tacactgaac tgaatccgtc gatcaactat
       61 acaagaaaca acctcagatg ggatgcctgg ggggcatata ctgaagattt ttttctaaag
      121 gatcatatta atgagattct gaacattctc aggaatgact ggggagtatc ctctcttccc
      181 agaacgccag gtgtaaaaat ggatgatctg aaagttccga agtccagact tgacaagaat
      241 ctccttgcag gactttcaaa aattcttcca gtgaaaactg atcttcagga aagagtattt
      301 cattctgccg gaagaagcta ttatgatgta atccgtctcc ggttcaatct tctgaaggat
      361 tatgttgacg gtgtcgtata tccgaagaca gaggaagaaa tccgttcact tcttcttttc
      421 tgcaagacta aaaaaatagc agttgttcct ttcggaggcg ggtcctcggt tgtgggagga
      481 gtggaagcca gaaaaggaaa acagcacaag gcggtcattt cagtggatat gaccgatatg
      541 aaagaacttg tggacattga cccagtcagc tgcactgcat cgtttcaggc gggcatttac
      601 gggccagttc ttgaacatat actgaatgaa aggggattta cactgggaca ttttcctcag
      661 tcatttgagt attccactct gggcggatgg attgcggcca gaagctccgg gcagcagtcc
      721 ggcaaatacg gccgtataga taaaatcatt aattcagcaa gaatcattac acctaacggg
      781 acaattgaaa catggaaggt gcctctttcc tctgcaggtc ctgatatgaa tcagataatc
      841 tcaggaagcg aagggctgct tggaatcatt acacaggtaa cactcaagat tcagcctttg
      901 ccgaaatcca gaaaatactt tggaatcgtt tttcctgatt ttgcaagcgg agtcggattt
      961 attcgggaag ccaacagaaa gggtctcaag ctttcgatga tgaggctttc agacaagatt
     1021 gaaacgaatc agtattccag gtttggtaca ataggcaagc cgatgactgc tttccgccga
     1081 ttcaagtatg caatgattga aaaaatcctt caatggaatg atattctcga caacagatgt
     1141 gcattgatcg gaggagtcga ttcagacgag attgaagtgg aaagacagat ccatgaggca
     1201 aaacagacag caaaacatca tagcggtttt tttgccggtg agggtccggg aaaaaactgg
     1261 ctgaagagcc ggtacaatat gccttttcta aggaatcatc ttcttgaaaa cgggataggt
     1321 gttgacactc ttgaaacagt catcacctat gacaggattc tgactcttca taagcatgtg
     1381 gaggaggcga tcaagaacac aatgcagtgc actgtcatgt gccatgtgag tcacagttat
     1441 gctgacggtg ccagtctgta ttttacgata atctttcctc tggatctgaa agatccctgc
     1501 ggtcagtgga taaaaatgaa aaaggctgct tcggatgcaa tctactcttc gggaggaagc
     1561 atcagtcacc atcatggtgt cgggacagac catcaggagt ggtacctgaa acaggcgggt
     1621 gaaacagcag tgaccgccct gaaaggactc aagaattctc tggataaaga gggaattatg
     1681 aatcctggaa aactgttt
//
LOCUS       DAOQGJ010000973         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51609_length_1698_cov_3.329884, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000973 DAOQGJ010000000
VERSION     DAOQGJ010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51609_length_1698_cov_3.329884"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="PK683_14175"
     CDS             complement(<1..1651)
                     /locus_tag="PK683_14175"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH09643.1"
                     /translation="MKNISFIENLYCIFYGVLLAMIFYNGSIFISLKNPSYLLYSLTT
                     LSMLIYQISFSGHGFQYLWPENLWMQKYTGLIAAVLFAVFAFEFMIRFLDLKRKTRIF
                     LRSWLLLQNLITCLALTDSLTSVFRVVGIAGILNTVTVITAGILSWRKGYRPARFFVI
                     GWSLYALGTILAILRLAGLIETNALTNYAMQIGVVSELMLLSIALADSYKLIAEEKSN
                     IQKELLNVQIRMVENLKTSESQLEERVALRTAELQESNNTLESALSNLKSAQAQLIQS
                     EKMASLGQLVANVAHEINTPIGAVKASGRNISDALEYTLENLPVLFNTLEKQDRELFM
                     KLIRPKNIQPAVLSTKEERQLIKETMRQLEEAGLIDTRDKAEMLVQLNVHKEVSEYMP
                     LLKHPEAEFILKAVRRISTIISSAANINTAVERVSKIIFALKSFSHTNISEQMVNADL
                     REGLEVVLTVYNNLLKQGIEVIRKYEEIQKIKCFPDELNQVWTNLIHNSVLAMSGKGR
                     LTIEVKQTETEVSVSISDTGVGIPENIRGRIFEPFFTTRPAG"
BASE COUNT          463 a          395 c          336 g          504 t
ORIGIN      
        1 ctccggccgg ccgggtagta aagaaaggct cgaagatcct ccctcgtata ttttcaggaa
       61 tacctacccc tgtatccgaa atagacaccg atacttcggt ttctgtctgt ttcacctcaa
      121 ttgtaagtct tcctttcccc gacattgcca gaactgagtt atgaatcaga ttcgtccaga
      181 cctggttcag ctcatccgga aaacatttta ttttctgaat ctcctcatat ttgcgaatca
      241 cttcaattcc ctgtttcagc agattattat agacagtcaa aacaacttcc agtccttccc
      301 gcagatctgc attcaccatc tgttcagaaa tattcgtatg tgaaaacgac ttcaatgcaa
      361 aaataatttt ggatactctc tccactgcgg tattgatgtt cgctgcactg ctgatgattg
      421 tggaaatccg tcttacagct ttcaagataa attctgcttc cggatgtttc aggagaggca
      481 tatactccga cacttcctta tgcacattca gctgaaccag catttctgct ttatcacggg
      541 tgtcgatcag gcctgcttcc tccagctgac gcattgtctc ctttatgagc tgtctttctt
      601 cttttgtact cagaactgcc ggctgtatat ttttaggacg aataagcttc ataaacagtt
      661 ctctgtcctg tttttccaga gtgttgaaaa gcacaggaag attttccagt gtatactcca
      721 gagcatcgga gatattcctt ccgctagcct tgactgcacc gatgggagta ttgatctcat
      781 gcgctacatt tgccaccagc tgtccgagag aagccatttt ttcggactga atcagctgtg
      841 cctgtgcgga ctttaaattg ctcaaggctg attccaatgt gttattgctt tcctgcagtt
      901 ctgcagttcg aagagcgact ctctcctcaa gctgactttc cgaagtcttt aaattttcaa
      961 ccatcctgat ttgaacattc agaagctcct tctgtatatt gcttttttct tctgcaatca
     1021 gtttataact gtcggcaagt gctatggaca gcagcatcag ctcagatact actccaatct
     1081 gcattgcgta attggtcagt gcatttgtct ctatcagtcc agccagtctc agtatggcaa
     1141 gtatggttcc taaagcataa agactccagc cgattacgaa aaacctggca ggccgatagc
     1201 ctttcctcca ggataaaatt cctgcagtta tgacagtgac tgtgtttaaa atacctgcaa
     1261 tgccgacgac tctgaataca gaagtcaggg aatcagtcaa tgcaagacag gtgatcagat
     1321 tctgaagcag cagccagctg cgtaaaaaaa ttctagtttt ccttttcagg tccaggaacc
     1381 ggatcataaa ctcaaatgca aagacagcaa acagaacagc ggcaatcagt ccggtatact
     1441 tctgcatcca gaggttttcc ggccaaagat attgaaatcc atgtccgctg aacgatatct
     1501 gatatatcag catagacagt gtggtgagag aatacagcag atatgacgga tttttaagag
     1561 atatgaatat ggatccgtta taaaaaatca ttgctagaag cactccgtaa aaaatacagt
     1621 acagattttc aataaaggaa atattcttca atacatgctt cttctgatgc agagtcagag
     1681 ggataatgga ggcgcttg
//
LOCUS       DAOQGJ010000974         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51613_length_1698_cov_3.209982, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000974 DAOQGJ010000000
VERSION     DAOQGJ010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51613_length_1698_cov_3.209982"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PK683_14180"
     CDS             <1..1024
                     /locus_tag="PK683_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH09644.1"
                     /translation="LNIVRKSGDLLLNIINDIMDFSKIESGQMQILPVPYSLKALMEN
                     ISDFIKILTAERKKNIIFRTFIDENISDAVMIDEHRVLQILNNLLSNSVKFTDRGFID
                     VKIELKKGFIEFSVSDTGKGISQDYYQHIFEPFSQEDISDTRTFGGTGLGLAICRKLA
                     ELMDGSLSFTSSTGPDSGTAFFLRLPYYPASKLFDHTEISSDVSETETLSSRKILLAE
                     DDLISQRLIKRLLSKAGCTVITADNGQEAVKAYTENEKIDLVIMDNQMPVKTGLEAIM
                     EIRNWENVKNPRTKIPIIMLSAAVFPEDIKRGSIAGADLYMTKPVNIHDLKRKIIRLL
                     QTMEAS"
     gene            1183..1578
                     /locus_tag="PK683_14185"
     CDS             1183..1578
                     /locus_tag="PK683_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09645.1"
                     /translation="MKLFPSMQFTIDSTMKPEEVRIRLSEYVMPLKMQFTLFYKEYEK
                     PFSGSYSEKEFELIKNIRYRNSFNPVIYGSISPYGSGSRIDVRLELHRIVKIIIIPHI
                     FMIFGYFLARYGFNREAGSIRETLTKILR"
BASE COUNT          568 a          319 c          348 g          463 t
ORIGIN      
        1 gctgaacatt gtcagaaaaa gcggcgatct ccttctgaac atcataaatg atattatgga
       61 tttttcgaag atagagtccg gtcagatgca gattcttccc gtgccttatt ctttaaaagc
      121 actgatggaa aatatctctg attttataaa aatacttaca gcagaaagaa agaaaaatat
      181 tatcttcagg acattcatag atgaaaacat ttctgatgcg gttatgattg atgaacacag
      241 agtgcttcag atcctgaaca accttctgag caattcagtc aagttcaccg acaggggttt
      301 tatagatgtg aaaattgaac tgaagaaagg attcattgag ttttccgtca gtgatactgg
      361 aaagggaatc agtcaggatt attatcagca tatatttgaa ccgttcagtc aggaagatat
      421 cagtgataca agaacattcg gaggaactgg actgggactt gccatctgca gaaaacttgc
      481 agagcttatg gacggaagtc tgagttttac ctccagtaca ggtccggatt caggaacagc
      541 attctttctc agattgcctt attatccggc atccaaactg tttgatcaca ctgaaatcag
      601 cagtgatgtt tcagaaacgg agacactcag cagcagaaaa attctgctgg cagaagatga
      661 tctgatcagc cagagactga taaagcgact cctcagcaag gcaggatgca ctgtcattac
      721 agctgacaac ggacaggaag cggtcaaggc atatacagag aatgaaaaaa tagatcttgt
      781 tattatggac aatcagatgc ctgtaaagac aggtctggaa gccattatgg aaattagaaa
      841 ctgggaaaac gtaaaaaatc cccgtacaaa gatccctatc attatgctgt ccgctgcagt
      901 ttttcctgag gatattaaaa gaggttcaat tgcaggagct gatctgtaca tgacaaaacc
      961 tgtcaatatt catgatctga agcggaaaat catcagactc cttcagacta tggaggcctc
     1021 ctgatttatt tcaatatccg aatgatccat tatgcagttc agagaatatt tctgaagcct
     1081 gcatttatga taaagattca gtgaagactg tccggttttt ttgatcgacc gtctgactcg
     1141 atttttataa tagtataata taattataaa ggatagattt caatgaaact ttttccctcg
     1201 atgcagttta caatagactc tacaatgaaa cctgaagaag tccgcataag gctttcagag
     1261 tacgtaatgc ctctgaaaat gcagtttacc ctgttttaca aggaatatga aaaacctttc
     1321 agcggttcct attccgaaaa agaatttgag ctgattaaaa acatcaggta ccgaaattca
     1381 ttcaatccgg tgatttacgg gtccatatca ccctatggaa gcggaagcag gattgatgta
     1441 agactggaac tgcacagaat cgtaaaaata atcattattc cgcatatatt catgattttc
     1501 ggttattttc ttgcaagata cggattcaac cgggaagcag gaagcatccg ggaaacactg
     1561 acaaaaatat tgaggtgact tatgaaaaaa caaattataa agatttttat cattatcggc
     1621 ctgactgctt ccgctgccct cccggcaaag gaagaaaaag acgtaatgca ctacttcaga
     1681 atactgcaca gagataca
//
LOCUS       DAOQGJ010000975         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51668_length_1697_cov_3.136419, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000975 DAOQGJ010000000
VERSION     DAOQGJ010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51668_length_1697_cov_3.136419"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PK683_14190"
     CDS             <1..1018
                     /locus_tag="PK683_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09646.1"
                     /translation="GNDSSTAGFTYWEYSRPGESSERSRNKGVVLGSQKQKFQFPASQ
                     WTRIGFTAGLIFGTRVEINPGEALDFVLGIFGLDIYKDDVYIYYFEPSEKIRKLMKSD
                     RVEEFAGLFEKEHLSGDIYVDEYNNTFLHYAAGDMSIKMVKYLIEQKRSDINKVSTLG
                     WSPMGHLTWCSGYSVAEKTSYEKENRVYGFADCPELMDYFESIGASYLYSGFMRDHSP
                     EIIDAVKNEKYTVIENYLYPSKRKSRIKSDGKICFDCVVFPHLEEIKALPGEGGNYDN
                     DSNKYGGMTLLQLAVYYDRPNVVKYLLVKGADPKKKNFRNADAYRFAEMLGRTEILQI
                     LPKR"
     gene            1133..>1697
                     /locus_tag="PK683_14195"
     CDS             1133..>1697
                     /locus_tag="PK683_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09647.1"
                     /translation="MKTTKSGFDISAPGGLLFGFALFNFTSQSSSGTYTIGGSITGIS
                     SGTLTLSYNSQANTIDLSAKTYKISAAKGAYTLSLSSVPTGYICTVSNSSGTLSADIT
                     NADITCSAVTVSSVYTGNSNWNDYVKNDGSSLLNATNTACTGSETGGYRACIHGGEIR
                     KITLTGLSSCTGITASDALKAFNWICRT"
BASE COUNT          506 a          324 c          389 g          478 t
ORIGIN      
        1 gggcaacgac agcagcactg caggatttac ctattgggaa tattcaaggc ctggcgaaag
       61 cagtgagcgt tctcggaaca aaggtgttgt tttagggagt caaaaacaga aattccagtt
      121 tccagcatca cagtggacaa gaatcggatt tactgcagga ctgatttttg ggactcgagt
      181 ggaaattaat ccaggcgagg ctctggattt tgtgctgggg attttcggtc tggatatata
      241 caaagatgat gtgtacatat attattttga gccctcggag aaaatccgca aactgatgaa
      301 atctgacagg gttgaggagt ttgccggtct ctttgaaaaa gaacaccttt ccggggatat
      361 ttacgtggac gaatacaata acacatttct tcattatgcc gcaggagata tgtcaatcaa
      421 aatggtaaag taccttattg aacagaagag atcggatatt aataaggtaa gcactcttgg
      481 atggagtcca atgggacatc tgacctggtg ttccggttac tctgtcgccg agaagacgag
      541 ttatgaaaag gagaaccgtg tttacgggtt tgctgactgt cctgaactta tggattattt
      601 tgaatctatc ggggcaagtt atctgtattc aggatttatg agagatcaca gtcctgaaat
      661 cattgatgca gtgaaaaatg aaaaatacac tgtgattgaa aactatttat acccctcaaa
      721 gagaaaaagc cgtattaaaa gtgatggaaa aatctgcttt gactgtgtgg tatttcctca
      781 tctcgaagaa atcaaagctc ttcctggtga aggcggaaat tatgacaatg attccaataa
      841 gtacggaggg atgactctgc tgcagctggc tgtatattat gatagaccga atgtcgtgaa
      901 atatcttctc gtgaaaggtg ctgatccgaa aaagaaaaat ttcaggaatg cagatgctta
      961 ccgttttgca gaaatgctgg gaagaacaga aattctgcaa attctgccta aaaggtagaa
     1021 ggtgaaaaat tattatttcc aaagaaaaaa atccgtattc tgactgttac tataatcagg
     1081 ggatactgtg agaaaactga ttctaatact tctgtgcatt tttttgggat gtttgaaaac
     1141 tacaaagtca ggatttgata tttctgcccc tgggggactc ctttttggat ttgcactatt
     1201 caattttact tcccagtctt catcaggcac ttacacaatc ggcggaagca ttacaggaat
     1261 cagttccggg actttgaccc tgtcttacaa ttcacaggcc aataccattg acctttctgc
     1321 aaagacctat aaaatcagtg ctgcaaaagg agcatataca ttatcattat cttctgttcc
     1381 gaccggctac atctgcaccg tttcgaattc ttccggaact ctttctgcag atattacgaa
     1441 tgcagacatc acctgttcag cggttactgt atcatctgtc tataccggaa actctaattg
     1501 gaacgactat gtgaagaatg acggaagctc tcttctgaat gcgacgaata ctgcctgcac
     1561 gggttctgag actgggggct acagggcctg tattcatgga ggcgagatca gaaaaatcac
     1621 tctgactgga ctcagttcct gcacaggcat tactgcgtct gatgcactca aggctttcaa
     1681 ctggatctgc agaaccg
//
LOCUS       DAOQGJ010000976         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51669_length_1697_cov_3.075518, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000976 DAOQGJ010000000
VERSION     DAOQGJ010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51669_length_1697_cov_3.075518"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK683_14200"
     CDS             complement(<1..523)
                     /locus_tag="PK683_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09648.1"
                     /translation="MRKLILISVFFFTLSSLYSQDKEVPAKKNKSDDSKKTEDSKKVS
                     AKDAKKPAVKDAKKAAAKDTKKTAAKDVKKTVAKDVKTPAAKETSEEKEKTADEADVK
                     EAPGKTGTGEETAKDVPKKEAGSEEESSSTDTAPTAKDTSPGAKDAGTDTADKPKEEP
                     AEKTSSGNLPAFRG"
     gene            complement(520..1122)
                     /locus_tag="PK683_14205"
     CDS             complement(520..1122)
                     /locus_tag="PK683_14205"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNH09649.1"
                     /translation="MKQIMKTTYGIAALAVLFAFSSLNADAVKIKEDKLKIESGKMLF
                     TLIKTMAGSESKMTGLASQMTGSFDMKAKTFDITMQINLNSFALGGEYKFANDRMHET
                     YLESSQYPSANYKGSILSYDPVSGKAKVKGTMTIHGTKKENVTIDGLVVPSASGSGYL
                     LTADFLVNLKDYNIAVPDIKLATVKEIVSLKMKIELRGAK"
     gene            complement(1141..1545)
                     /locus_tag="PK683_14210"
     CDS             complement(1141..1545)
                     /locus_tag="PK683_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09650.1"
                     /translation="MTKNSWAISAVLLILLSGINCADKKYSDIEKQKIIQGIIVGKVT
                     GSSGSAISCTSSFTFSGLGLSSKCGSCHSGGNLGGGLDVTSYNSTIARVSSGSPEGST
                     LYSKINGGSMSSYSDTTTNNAVYCWIKAGAAQ"
BASE COUNT          394 a          378 c          312 g          613 t
ORIGIN      
        1 gtcctctgaa tgcaggcaaa tttccgctgg aagttttttc tgcaggctct tccttcggtt
       61 tatccgctgt atctgttcct gcatctttgg ctcccggcga agtatctttt gcagtcgggg
      121 ctgtatccgt tgagctggat tcttcttcag aaccggcttc tttcttagga acatcctttg
      181 cagtttcttc tcctgtccct gtcttaccag gtgcttcctt cacatctgct tcatcagctg
      241 ttttttcttt ctcctcagat gtttccttgg ctgccggtgt cttcacatct tttgcaacag
      301 tcttttttac atctttcgct gctgtttttt ttgtatcctt tgctgcagct ttttttgcat
      361 cttttactgc aggtttcttt gcatccttgg cactgacttt ttttgaatct tccgttttct
      421 ttgaatcatc agatttattc ttttttgcag gaacctcctt gtcctgtgaa tacaaggagg
      481 agagtgtaaa aaagaaaact gatatcagaa tcagttttct cattttgcac ctctcagttc
      541 aattttcatc ttaagactta caatttcttt tactgtcgca agcttaatat caggaactgc
      601 aatgttatag tctttcagat tgactaggaa atcagcagtg aggagatacc cggaaccgga
      661 agctgaagga acaactagac cgtcaatggt cacattttct ttctttgttc catgaattgt
      721 cattgttcct tttacttttg cctttcctga aacaggatca taagataata tgcttccttt
      781 ataatttgca gaaggatact gagatgattc caggtaggtt tcgtgcatcc tgtcatttgc
      841 aaatttgtat tctccgccga gtgcaaaact gttcagattg atctgcattg tgatatcaaa
      901 tgttttggcc ttcatatcaa aagaacctgt catctgactg gcaagccctg tcattttgga
      961 ttcagaaccg gccattgtct tgataagtgt aaaaagcatc ttgcctgatt caattttaag
     1021 tttatcttcc ttgattttta ctgcatcggc attcagtgaa ctgaatgcaa aaagtacagc
     1081 cagtgctgcg attccgtaag ttgttttcat aatttgtttc atagtatagt ctcctgtaat
     1141 ttactgtgct gcccctgctt tgatccagca gtatactgca ttgttagttg tggtatcaga
     1201 gtatgaggac atgcttccgc cgttaatttt gctgtaaagt gtgcttcctt caggacttcc
     1261 tgaggatact ctggcaatcg ttgaattgta acttgtcaca tccagaccgc ctcctagatt
     1321 gcccccgctg tgacagcttc cgcatttcga ggaaagtccc aggcctgaga aggtgaaaga
     1381 tgatgtacag ctgattgcag aaccgctgct tcctgtcact ttcccgacaa tgatgccttg
     1441 aatgatcttc tgtttttcta tatcagaata ttttttatct gcacagttta tccctgataa
     1501 taatatcagt aagactgccg atatcgccca actgttttta gtcatgcacc gcccccgaat
     1561 cctctaaata tcttactgaa aactaaactg attcaaaaaa tcttttccag caatctaaaa
     1621 gaattatact ctgttacagc ttcatgtcaa gaattaatta atgctttctg aactgactgc
     1681 acctgttcat gtactgt
//
LOCUS       DAOQGJ010000977         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51708_length_1696_cov_2.737355, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000977 DAOQGJ010000000
VERSION     DAOQGJ010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51708_length_1696_cov_2.737355"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..664
                     /locus_tag="PK683_14215"
     CDS             137..664
                     /locus_tag="PK683_14215"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HNH09651.1"
                     /translation="MEEINIHLFQEKIQNSFAMEGSGVSTLLIPKDLINRFNRRLKIK
                     GGKTKYFGYLIGLYRTMLRSFCYDASSGVRTEYQWKNQELQKINFRPQNNDWAELGAF
                     SAASGKSRCLLFVYLLLMDLNGWGAILKLAGITAEYTRSEKHEWELLGAFGLNRDRNE
                     YRRELHPKIIFTETG"
     gene            complement(675..1346)
                     /locus_tag="PK683_14220"
     CDS             complement(675..1346)
                     /locus_tag="PK683_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HNH09652.1"
                     /translation="MHKEKGGSSVMCGRYGLPTKISKAVENLEFYREIARLRDRIPKQ
                     NEAYPGSKVPVVRKRLTMNTVEFIQWGIKPDWADKTLINARKENIMSSNFWRNFFLQE
                     RCLIPASYFVEWQMSEGKKIPWQVSLNDKEMFFFAGFIVKLQDKKNENTESCVILTEG
                     ANSLMKEIHNYGPNRHRQPLILSDEKHESWISNSDLTDMLSEESLSSENMKASKVNDN
                     QALLF"
     gene            complement(1339..>1696)
                     /locus_tag="PK683_14225"
     CDS             complement(1339..>1696)
                     /locus_tag="PK683_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNH09653.1"
                     /translation="GNIEWQDLLIRKTAGKSLEEYEMDTGTSLSMQELQEYGIEIQAG
                     EKVKYVVVSQKNKNKSLRYLSEEKALAEQNKNAVFDLKHYEKLLMNAFEEVWQYFAYP
                     EYFQEVRERQKFILFA"
BASE COUNT          508 a          335 c          293 g          560 t
ORIGIN      
        1 cttcagtcac tgtcctgaaa aagcattgta ggatctcccc tcgagcaaag tcatatccga
       61 acaagaaata cgtttaaggt gcaaaaagat cgattttttt atgggatacg atcattttta
      121 aacaggtata ttttgtatgg aagaaataaa cattcatttg tttcaggaaa aaatacaaaa
      181 cagctttgcg atggaaggaa gcggagtttc aactcttttg attcccaaag atctcataaa
      241 tcgttttaat cgacgactga aaatcaaggg agggaaaaca aagtatttcg gttatctgat
      301 tggactttac agaactatgc ttcgttcttt ctgctatgat gcatcatctg gtgtaagaac
      361 ggagtatcaa tggaaaaatc aggaacttca aaaaataaat ttcagaccgc agaataatga
      421 ttgggcagaa ctgggagctt tttctgcagc ttcaggtaaa agcagatgcc ttctattcgt
      481 atatctcctt cttatggatc tgaatggatg gggagctatt ctgaaacttg caggcattac
      541 tgcagagtat acccgatccg aaaagcatga atgggaactg ctgggtgcat ttgggttaaa
      601 ccgggacaga aatgaatacc gaagggaact tcatcccaaa attattttta cagaaactgg
      661 ataatatttt tttgttaaaa taataaagct tgattatcat ttactttaga agctttcata
      721 ttttcagatg aaagactttc ctcggacagc atatcagtca agtcagaatt actgatccat
      781 gactcatgtt tttcatcgga aagaatcagc ggctgtctgt gcctgttcgg accatagtta
      841 tgaatttctt tcattaagga atttgctcct tcagtcagga tgacacagga ctcagtattt
      901 tcattttttt tatcctgcag tttgactata aagcctgcaa aaaagaacat ttctttatca
      961 ttaagactga cctgccaggg aatctttttt ccttctgaca tctgccactc cacaaaatat
     1021 gatgcaggaa tcagacatct ttcctgaaga aagaaatttc tccaaaagtt tgaagacatg
     1081 atgttttctt ttcgtgcatt tatcagggtt ttatctgccc aatcaggttt tattccccac
     1141 tgaatgaact caaccgtatt cattgtcagc cgttttctga ctacgggaac ctttgatccc
     1201 ggataagcct cattctgctt cggaattcga tctctcaatc ttgcaatttc tctataaaat
     1261 tccagatttt cgactgcctt agaaatcttt gtaggaagtc cgtaccttcc gcacataact
     1321 gaagaacctc ctttctcttt atgcaaaaag aataaatttc tgcctttctc tcacttcctg
     1381 aaaatactca ggatacgcaa aatactgcca aacttcttca aaagcattca taagcagttt
     1441 ttcataatgc ttaagatcaa atactgcatt tttattctgt tctgcaagtg ctttttcttc
     1501 agagagataa cgtaatgact tatttttatt tttctgagat acaacaacat atttaacctt
     1561 ttctcctgcc tggatctcaa taccatattc ctggagttcc tgcattgaaa gagatgttcc
     1621 tgtatccatt tcatattctt caagtgattt tcctgcagtc tttcttatta gaagatcctg
     1681 ccattcaata ttgccc
//
LOCUS       DAOQGJ010000978         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51713_length_1696_cov_2.526508, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000978 DAOQGJ010000000
VERSION     DAOQGJ010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51713_length_1696_cov_2.526508"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..644)
                     /locus_tag="PK683_14230"
     CDS             complement(99..644)
                     /locus_tag="PK683_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09654.1"
                     /translation="MRPIFTVHAGEYLVGSHFEKNYRNINVWVPSKDTGTDILLSSND
                     NRKTVSIQVKYSKDFLITHLGSHFQKQLKSFGWWTLKQSKIRASKSDYWVFALHSFNG
                     KNIEYVIIKPADLLARLKKLHGNSDTIQMYLWITKGKKCWETRGLNKPEQEAIAKDTY
                     SNAERDFTQYLNDWDSITKNL"
     gene            complement(655..>1696)
                     /locus_tag="PK683_14235"
     CDS             complement(655..>1696)
                     /locus_tag="PK683_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09655.1"
                     /translation="ENNDALKEINVQLEEIGKGIGKFGQAIKEQTKLHKEHYDELKKE
                     KALKEVLYNIKKYFKSLETVNDMAARAYGAKTLLNFLNTGDLKTKDLSDIKDKEFYDE
                     QLESAENSLKNLSAEVRKELEDFEKLYSSYYGLKEADLRKEFPKYQKLNEYYEADYTK
                     IEDDKPQPPMEIKRLDSVDWNAIEGSLENQKFAKVTGYATVGLFILMISFCREKSSQE
                     IMAGITSIPLWAWIYFRYKISTFKTDNLSQYQKSDYKHLVKMREEYNQRVQCYEAQAS
                     EKKSELNAEVGTLNKKMELENDAIVKYNKSIDEELADAEQNRRKILETMKSDINSYLD
                     SHPKIQEFLPKV"
BASE COUNT          438 a          369 c          273 g          616 t
ORIGIN      
        1 ggctcggggc gagaactttc acatctgtcc gtgaaagttc gatgcggctc gcgctcttgt
       61 tgaccgaagt tcataaagaa agatgatggt tgcagtactt aaagattttt tgtaattgaa
      121 tcccagtcat tcaaatattg agtaaaatct ctttcagcat tactgtatgt atctttggct
      181 attgcttcct gttctggctt atttaagcct ctcgtttccc aacatttttt acctttagta
      241 atccaaagat acatttgaat tgtatcagaa tttccgtgca gtttcttaag cctggcaaga
      301 aggtcagcag gtttaataat aacatactca atgttctttc cattgaaact gtgaagtgca
      361 aatacccaat aatcactttt tgaagccctt attttgcttt gcttcaatgt ccaccatcca
      421 aaagacttca gctgcttctg aaaatggctt cctaaatgtg taattaaaaa atcttttgaa
      481 tattttacct gaattgacac agtctttctg ttatcgttac tggacagaag aatatctgtc
      541 cctgtatctt ttgaaggaac ccagacatta atgtttctgt aattcttttc aaagtgcgaa
      601 cccactaaat actctccggc atgaactgta aaaattggtc tcatcgcttt ctcctcatac
      661 cttaggtaaa aattcctgaa tcttcggatg actgtctaaa taggaattta tatcagattt
      721 catcgtttcc aggattttcc gacggttttg ctcagcatca gcaagttctt catcaataga
      781 cttattgtat ttgactattg catcattttc aagttccatc tttttgttca aagtccctac
      841 ttcggcattc aattcacttt ttttttcgga agcctgagct tcatagcact gcacccgctg
      901 attgtattct tctctcattt tgacaagatg cttataatcg ctcttctgat attgggaaag
      961 attatctgtt ttgaaagttg aaattttgta cctgaagtat atccaagccc acagagggat
     1021 agaggtaatt ccggccataa tttcctgtga tgacttttct cggcagaaac tgatcatcag
     1081 aataaaaagc ccaactgtcg cataccccgt aacctttgca aacttctgat tctctaaaga
     1141 accctctatg gcattccagt caacggaatc aagtcgcttt atttccatcg ggggctgagg
     1201 tttgtcatcc tcaatctttg tatagtctgc ctcatagtat tcgttcagct tctgatattt
     1261 cgggaattcc tttctgagat cagcttcttt caggccatag tagcttgaat acagcttttc
     1321 aaagtcttcc aattctttcc tgacttccgc acttagattc ttcagtgaat tttcagcaga
     1381 ttcaagctgc tcatcataaa actccttgtc ttttatgtct gacagatctt ttgtcttcaa
     1441 atctcctgta ttcaaaaaat taagaagcgt cttggctccg tatgctctgg cggccatatc
     1501 attgacagtt tccaatgatt taaaatattt tttaatattg tataacactt ctttcagagc
     1561 tttctctttc ttcagctcgt cataatgttc cttatgcagt tttgtctgct cctttatggc
     1621 ctgcccaaat tttccaatac cttttccgat ttcctccagc tgaacattta tttctttcaa
     1681 ggcatcatta ttttca
//
LOCUS       DAOQGJ010000979         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51764_length_1695_cov_2.823780, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000979 DAOQGJ010000000
VERSION     DAOQGJ010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51764_length_1695_cov_2.823780"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PK683_14240"
     CDS             <1..660
                     /locus_tag="PK683_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09656.1"
                     /translation="KINVEKPSDGGNAAAKLVGAAPSTLKLASKVIPLLYNTCLKYGV
                     TDLGQIAYVIATAEHESKMGEWMKEGIFEKDEAKRNANFEKRYQGRADLGNTNPGDGI
                     KFHGRGFCQITGRVNYTRWTKIFQNKLGRADIDLVENPDQAADTGIAAIILVTGMRDA
                     GFTKHKLSDHINGSNRDFKNARRIINAPDNPGKPDDAEKKAATAKIAQAAEKYYRALI
                     S"
     gene            799..1476
                     /locus_tag="PK683_14245"
     CDS             799..1476
                     /locus_tag="PK683_14245"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNH09657.1"
                     /translation="MINIKKNTHYGSVHLKVLGRNELPDMDYKSDTVWKDSVREASKI
                     LGLPEENIFLVRQTHSTEGYEAPFETSEGFPEGDWIFTRKEKTAVLIRTADCMPVFFY
                     SRTKPLVGIIHAGWKGTAAGIVPKVLDFLGEHLSSADFFLGPCIRRDRYEVQTDVSSL
                     FENYPEAVSVKDAKIQLGLEYVLKKQLEGRQFEDSKICTFDDPDYFSHRGKDSGRNWN
                     IIYSEFY"
BASE COUNT          545 a          318 c          402 g          430 t
ORIGIN      
        1 aagatcaatg tagaaaaacc ctcagacgga ggaaatgcgg ctgcaaaact cgtcggtgcg
       61 gctccttcca ctttaaagct tgcttccaaa gtgattcccc ttctgtacaa tacctgtctg
      121 aaatacggag tgactgatct ggggcagatt gcctatgtca ttgccactgc agaacatgag
      181 tccaaaatgg gcgaatggat gaaagaaggc atctttgaaa aagatgaagc aaagagaaat
      241 gccaatttcg aaaaacgcta tcaggggcgt gctgacttag gaaatacaaa tcctggtgac
      301 gggatcaaat ttcatggaag aggattctgt cagatcacag gcagggtcaa ttataccagg
      361 tggacaaaaa tatttcagaa caagcttggc cgtgcagata ttgatcttgt cgagaatcct
      421 gaccaggcag cggatacagg gattgcagcg atcatccttg ttacaggaat gagagatgca
      481 ggttttacca aacataaact ttcagatcat atcaacggtt ccaacaggga ttttaaaaac
      541 gccagacgaa tcatcaatgc acctgacaat ccaggaaaac cggacgatgc agaaaaaaag
      601 gctgccactg caaaaatcgc acaggcagca gagaaatact acagggcatt gatttcgtag
      661 ctaagtgcaa ttgcatacga tatgaagtaa gtaaaaatcg ggatcttaag ggacgaagtc
      721 ccttaaaacc gccggtaggc cccagccaaa tgaataactg cgaagccttt aggctgagca
      781 gttaagttta agttggatat gattaacata aaaaaaaata cacactatgg cagtgttcat
      841 ttgaaggttc tggggaggaa tgaactccct gatatggatt ataaatcgga tactgtatgg
      901 aaagattctg tcagagaggc atctaaaatt ctcggccttc ctgaagaaaa tatttttctt
      961 gtaaggcaga ctcacagcac agaaggatat gaggctcctt ttgaaacctc tgaagggttt
     1021 ccggaagggg actggatttt tacgaggaag gaaaaaacag ctgttctgat caggacagcc
     1081 gactgtatgc ctgtgttttt ttattccaga acaaaacctc ttgtggggat cattcatgca
     1141 ggatggaagg gaactgctgc tggaattgtt ccaaaggttc tggattttct cggagagcat
     1201 ctttcttctg cagatttttt tttagggccc tgcatacgtc gagacagata tgaggtgcag
     1261 actgatgtat cgagtctgtt cgaaaattac ccggaggctg tatcagtgaa agatgccaaa
     1321 atacagctgg gtctcgagta tgtgctgaaa aaacagctcg aaggcagaca gtttgaggac
     1381 tcaaaaatct gcacttttga tgatccggac tacttcagtc atagaggaaa agattccggc
     1441 agaaattgga atattattta ctcagagttc tactgatgcg acttcacaat ccaaaccagg
     1501 aaacagaata ctgagagaaa tctgagtatt tattttgaat ttataagata atgagataat
     1561 atcccgtcaa aaactacctt gtcctgccct gtctttgcct tgcattccag agtgattttg
     1621 gatctggaat gaatgatgta tggatttgcg aagaaaagtt ttacgaaaaa acttaagacg
     1681 gaagagatgt ttgcc
//
LOCUS       DAOQGJ010000980         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51770_length_1695_cov_2.428049, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000980 DAOQGJ010000000
VERSION     DAOQGJ010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51770_length_1695_cov_2.428049"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK683_14250"
     CDS             <1..233
                     /locus_tag="PK683_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HNH09658.1"
                     /translation="LISNDKQKKVTKIPLFGDLPILGSFFRRSTSQNVRTNLMVFITP
                     HILGDRAKADRITAEKRMEQERIEEEREKQLR"
     gene            230..>1695
                     /locus_tag="PK683_14255"
     CDS             230..>1695
                     /locus_tag="PK683_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNH09659.1"
                     /translation="MRKTLGEILVEDGVITEKELSESLKAQAKSHLPLGQIIQKKGIA
                     GEMDIMKALSKIHNLEFVEKIDFHNYDDIYKLVPYKLIQKSRMIPYQYSGDVVWVAVT
                     DPTDLHPMDDFRNFLRGYKVKFVLAPEPEILRIIHSHFDKTASNAKEMMDEMVDGELS
                     DLAGSFESEDALDLSNEAPIIKMVNVILSQAVTERASDIHIESYEKSVSVRYRVDGVL
                     HKVLSPPKSYQAGIISRIKIMSNLNIAENRLPQDGRIKLKLAGKEIDVRVSTLPCQYG
                     ERVVMRLLNKTDQKYSMSTLGFTKKLQDEFTELIYHTHGIILVTGPTGSGKSTTLYSS
                     LSELNSPERNIITCEDPVEYQFDGISQVQMQEKIGLTFAAALRSILRQDPDVVMVGEI
                     RDQETARIAIQASLTGHLVFSTLHTNDAASSVTRLVDMGIEPYLITSSVLGFVAQRLV
                     RVNCKNCKKPYEPSDKELESLDIKREELIDGVLHRGAG"
BASE COUNT          531 a          376 c          392 g          396 t
ORIGIN      
        1 gactgatttc aaatgacaaa cagaaaaaag tcaccaagat ccctcttttc ggagatctgc
       61 ctattctggg atcttttttc agaagatcca caagtcagaa tgtcagaaca aatctgatgg
      121 tattcatcac acctcatatt cttggcgacc gtgcaaaggc agacagaatc actgctgaaa
      181 agcgtatgga acaggaaaga attgaagaag aaagggaaaa acagctcaga tgagaaagac
      241 actcggagaa attcttgttg aagacggagt catcacagag aaggaactca gtgaatcatt
      301 aaaagcacag gcaaaaagtc atctccccct gggtcagatc attcagaaga agggaatcgc
      361 gggagagatg gacattatga aagctctctc caaaatccac aatctggagt ttgtggaaaa
      421 gatagacttt cataattatg acgatattta taaactcgtc ccttacaaac tgattcagaa
      481 aagcaggatg attccatatc agtattcagg cgatgtagtc tgggtggcag tcacagatcc
      541 caccgatctg catcctatgg atgatttcag aaattttctg agagggtaca aggtaaaatt
      601 tgtactcgcc cctgaacctg aaattctgag aatcattcat tctcatttcg acaaaactgc
      661 atcgaatgca aaagagatga tggatgaaat ggtggacggg gaactgtccg acctggcagg
      721 aagttttgaa tcggaggatg cactcgatct cagcaacgaa gcaccgatca tcaaaatggt
      781 aaacgtcatc ctctctcagg cagttacaga aagggcctca gacattcata ttgaatctta
      841 cgaaaaatcg gtatctgtcc ggtacagggt ggacggggtt ctgcataaag tcctctcccc
      901 tcccaaatcc tatcaggccg gaatcatttc ccgtatcaaa attatgtcca acctgaacat
      961 tgcagagaac agactgcccc aggatggaag gatcaaactg aaactggccg gaaaggaaat
     1021 agatgttcga gtatccactc ttccctgtca gtacggtgag agggtggtga tgcgtcttct
     1081 gaacaaaacg gaccagaagt attccatgag cacgcttgga tttacgaaaa aactgcagga
     1141 tgagtttacc gaactgatct accatacaca cgggatcata ttagtcacag gtccgactgg
     1201 atcgggtaaa tccacaacac tttactcctc actttccgag ctgaacagtc ccgaaagaaa
     1261 catcatcacc tgcgaggatc cagtggaata ccagtttgac ggaatttcac aggtgcagat
     1321 gcaggaaaaa ataggactca ctttcgcagc agccctccgt tccatcctga gacaggatcc
     1381 ggacgtggtg atggtcgggg agatccgtga ccaggagaca gcccgaattg caattcaggc
     1441 atcattgact ggtcacttgg tattttccac acttcacaca aatgatgcag cctcctctgt
     1501 cacgcgtctt gtcgatatgg gaatagaacc ttatctgatt acctcttcgg tgctcggatt
     1561 tgtagctcag cgtcttgtca gggtcaactg caaaaactgc aaaaagccgt atgaaccttc
     1621 ggacaaggaa ctcgaatcac tggatatcaa aagagaagaa ctgattgatg gagtgcttca
     1681 cagaggtgca ggatg
//
LOCUS       DAOQGJ010000981         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51805_length_1694_cov_3.158633, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000981 DAOQGJ010000000
VERSION     DAOQGJ010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51805_length_1694_cov_3.158633"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PK683_14260"
     CDS             complement(<1..476)
                     /locus_tag="PK683_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09660.1"
                     /translation="MRISALHFSLILGLAAFYGCQPGKPGAVIKAILPGIPDTPGIEI
                     KAVTGLLTSEAGASAKFSVRLLSKPKSDVTIPNISTSNSKEGISDISSITFTPADWDR
                     KKTVTVTGVDDSLEDGSQKYSLNFGRSVSTDSDYSSIALSSFEIINADNDTSGITVS"
     gene            complement(636..1043)
                     /locus_tag="PK683_14265"
     CDS             complement(636..1043)
                     /locus_tag="PK683_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH09661.1"
                     /translation="MKQNFLLDTDTVSFFLKKDPSVAEKVMLAVQNESELGISIITHY
                     EILSGLKFKFSEKYLKSYSLFADSIYIYPVTEQSVQISSDLYSGLRKKGKPLDDIDLL
                     IAGTAIENRSILVTGNLSHFRRIKGLKTENWRE"
     gene            complement(1040..1315)
                     /locus_tag="PK683_14270"
     CDS             complement(1040..1315)
                     /locus_tag="PK683_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09662.1"
                     /translation="MKTERIVRADSKGRILLGADYAGSFLGIQSEGEGLFLRKMQVIP
                     LKNEKKKDIRNIVQFSGIWEDMSEEKFESFLTEVSERRKISSKRKKL"
BASE COUNT          453 a          340 c          351 g          550 t
ORIGIN      
        1 gatacagtga ttcctgaagt gtcattgtcc gcatttatga tttcaaaaga ggagagtgca
       61 atactgctgt aatctgaatc agtgctgaca gatcttccga agttgagaga atatttctga
      121 gaaccgtcct caagggagtc gtcgacgcct gtcactgtaa cggttttttt cctgtcccag
      181 tctgcgggag tgaatgttat tgaggatata tctgaaattc cttcctttga attgcttgta
      241 cttatgttcg gaattgtcac atccgatttc ggtttggaaa gaaggcggac agaaaatttt
      301 gcagatgctc ctgcttcgga agtcagaagg cctgtgacag ccttgatctc tattccgggt
      361 gtatccggaa tgcctggaag aattgccttg atgacagcac ccggcttccc tggctgacat
      421 ccatagaatg ccgccaatcc gagaatgaga ctgaaatgca gggctgagat acgcatatgc
      481 atagcattca gcagcagtat gttatttttc aataattttt tttatattcc gatcatttat
      541 aaaacttctt tcgcgagatc ctgaagcaaa tttacataaa aatatggcgg ctgatgtttt
      601 tttgcctgct ttgatgaaac agaggaaaag tcttcctatt cccgccagtt ttctgtcttg
      661 agtcccttga ttctacggaa atgagaaagg tttcctgtaa caagtatgct tctgttttca
      721 attgcagtgc ctgctatcag aaggtcaatg tcgtcaaggg gttttccttt ttttctgaga
      781 cctgaataaa gatctgaaga gatttgtacg gactgttccg ttactggata aatgtaaata
      841 ctgtcagcaa acagactgta ggatttcaga tatttttcgg aaaatttaaa cttcagtccg
      901 gacagaattt cataatgagt gattatgctg atgccgagct cagattcatt ctggacagcc
      961 agcattactt tttccgcaac agaaggatct tttttcagaa agaaggatac tgtgtccgta
     1021 tccagaagaa agttctgttt caaagtttct ttctctttga ggaaatcttt cttctttcac
     1081 ttacctcggt cagaaaactt tcaaattttt cttctgacat atcttcccag attcctgaaa
     1141 actgaacaat gttgcggatg tccttctttt tttcattttt caaagggatc acctgcatct
     1201 tcctcagaaa cagcccttct ccctcagact gaatccccag gaatgaacct gcataatctg
     1261 ccccaagaag tattctgcct ttactgtctg cgcggacaat cctttctgtc ttcatggaaa
     1321 taattttata taatgtggga attttgtcaa gtgggaaact cccacttaaa gaatatctgc
     1381 tgacttcaat cggaggttcc ctgaattcct tggacagaat ctgagaaatg tcatcagcac
     1441 ggtttggaaa aattcagatt cggtgaatca ggttctgaat acgttaggtt tcaattgcgg
     1501 gatgactttt cattaaaaaa atgaatctgc aatgagattt tcgtctcatt tcgactcaga
     1561 attgtgcttg ttttttacag cacagatgca ttcataaacc tgtgtatcca aaaggttttt
     1621 acggtttcgg aagaaacatc ggcatcaagg atgagacact cgattttgcg gtgattttct
     1681 ctgaggttcc ctgc
//
LOCUS       DAOQGJ010000982         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51873_length_1693_cov_2.548230, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000982 DAOQGJ010000000
VERSION     DAOQGJ010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51873_length_1693_cov_2.548230"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1693
                     /gene="infB"
                     /locus_tag="PK683_14275"
     CDS             <1..>1693
                     /gene="infB"
                     /locus_tag="PK683_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNH09663.1"
                     /translation="QGGGGYKGGGQGGGFTQPPKDNLPIDTSPSTNRKRNPNSAFDKK
                     EKTSVEGLENSKFFKQNIKKQQIPMSGTTVPKEISIMENIQVGELAKKLNLKPNDVIA
                     KLMKMGMMVTINNVIDSDTASLLADEYGCKVRIVSLYEETVIQEEQENQEDYIHRPPV
                     VTIMGHVDHGKTRLLDTIRETAVIDTEAGGITQHIGAYQVITPKGAITFLDTPGHEAF
                     TAMRARGASVTDIVVLVVAADDGVKPQTVEAISHAKAADVPIIVAINKIDLPSANPDR
                     VMQELVGHSLTPEEWGGSTIVCKISAKQKVGIEKLLEMILIQAEVLELDANPKRKAKG
                     TIIEAKLDPGRGPVATILIQNGTLRIGDPFLAGVYSGKVRAMYDDFGHNLKEAPPSCP
                     VLVTGLDSVPDAGDPFDVIGDEKEARYISQHRKEFQRIGQSSNLIRVTLDNMNEFIQQ
                     GALKELKIIIKSDVRGSSEAIKEALEKLSNADIKLTVIYAGTGQIVDSDIMLASASNA
                     LIVGFHVRANPRTLALAEKEGIQIKYYSIIYDVVNDIKHSMEGMLEPDKIENVIGS"
BASE COUNT          527 a          335 c          410 g          421 t
ORIGIN      
        1 caaggcggag gcggctacaa gggcggcggt caaggcgggg ggttcacaca gcctcctaag
       61 gataatctgc ctattgatac ttctccatcc accaacagaa agagaaatcc gaacagtgca
      121 tttgataaga aagaaaagac cagtgtagag ggtcttgaga attctaaatt tttcaaacag
      181 aatattaaaa aacagcagat tccgatgtcg ggaacaactg tacctaaaga aatttccatt
      241 atggaaaaca ttcaggtcgg tgaactggca aaaaaactga atctgaaacc gaatgacgta
      301 atcgccaaac tgatgaaaat gggaatgatg gttacgatca acaatgtgat cgacagtgat
      361 actgcttctc ttcttgcaga tgagtatgga tgcaaggtca gaattgtttc tctttacgag
      421 gaaactgtca ttcaggaaga gcaggagaat caggaagact atattcatcg tcctcccgtc
      481 gttactatta tgggacatgt ggatcacggt aaaaccagac ttctcgatac tatccgtgaa
      541 actgcagtca ttgatactga ggcgggaggg attacccagc atatcggagc ttatcaggta
      601 attacaccga aaggggcaat cactttcctg gatactcccg gtcacgaagc atttactgcg
      661 atgagagcaa ggggtgcttc tgtaactgac attgtagtgc ttgttgttgc tgcggatgac
      721 ggtgtaaaac ctcagactgt ggaagcaatc agccatgcaa aggcagccga cgttcctatc
      781 atcgttgcaa tcaacaagat tgatcttcct tctgcaaatc ctgacagagt aatgcaggaa
      841 cttgtaggac acagtctcac acccgaagaa tggggtggaa gcactattgt ctgcaaaatt
      901 tctgctaaac agaaagtggg aatagaaaaa cttctcgaaa tgattctgat tcaggccgag
      961 gttcttgaac tggatgccaa tccaaagaga aaagcaaaag gaacgattat tgaagcaaaa
     1021 ctggatccgg gcagaggtcc tgttgcgaca atactgattc agaatgggac actcagaatc
     1081 ggggatccat tcctggcagg tgtttattca ggaaaggtcc gtgcaatgta tgatgatttc
     1141 ggtcacaatc tgaaagaagc tcctccatcc tgtcccgtac ttgtcacagg tctggattct
     1201 gttccggatg caggtgatcc gttcgatgtg atcggggatg aaaaagaagc cagatacatt
     1261 tctcagcaca ggaaagaatt ccagagaatc ggtcagtctt ccaatctgat cagagtgact
     1321 ctggacaata tgaatgagtt catacagcag ggtgctttga aggaactgaa gatcatcatc
     1381 aagtccgatg tgagaggttc ttctgaagcg atcaaagaag ctttggagaa actttccaat
     1441 gctgatataa aactgactgt gatttacgcc ggaactggac agattgtcga ttcagacatt
     1501 atgcttgctt ctgcttcgaa tgcattgatc gtaggctttc atgtccgtgc aaatccgaga
     1561 acacttgccc ttgctgaaaa agaagggata cagattaaat actacagcat tatttatgat
     1621 gtagtgaacg atatcaaaca ttctatggaa ggaatgcttg agcctgacaa aattgaaaac
     1681 gtcattggaa gtg
//
LOCUS       DAOQGJ010000983         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51917_length_1692_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000983 DAOQGJ010000000
VERSION     DAOQGJ010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51917_length_1692_cov_3.000000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PK683_14280"
     CDS             <1..643
                     /locus_tag="PK683_14280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH09664.1"
                     /translation="DSSSAEWFLYGWMVFTVLGILNILYHLHYLPYNIIIVYSFVFYV
                     PLDFSILNIAMFKRVKSIELENILNQEKADILETELIKIKSRTKYEKSHIRGLNREKI
                     AGQLKNLMETEKIFLDEDLRSGDLAAMLRLTPHQFSEFLSEVMETSFHRLIQEYRIKE
                     AMILLRQDKNRNVLNIGLDVGFQSKSAFNSAFKKITGMTPMQYKNQTADSSAS"
     gene            complement(585..>1692)
                     /locus_tag="PK683_14285"
     CDS             complement(585..>1692)
                     /locus_tag="PK683_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09665.1"
                     /translation="PLHRRRLFRSRVDVRKDAVDGEFLASGCTNVDFNDPIIRAFNMV
                     NRSETMILGVGHDHLKITTPAGSVTEKKVSLPDKWIRGLGNVQYYLSQMESAFQMTRI
                     QAVELFKALPAGAVKSDYFLTENTNGYFLSPIEKENSVRLGGVHRLNLIRTLLPFIES
                     VSVYRDPEEQCSAFNVHFKDVNMLFVFSAGIYRGFSGEGKNLENLTEEIPEEWIIGIS
                     SLFRTNETFNPKELVLENSLSQEMMINALASLYSIGLLGFDLDSRKHYYRRLPFKLSR
                     LLSFNPRLVKAKKAAERDEVRILNRTEHLIEAEVKGSGDVVHRVLIQNNKPRCTCKWY
                     TDHETSRGLCRHILSVRMHLSQQSDSYTASESSL"
BASE COUNT          486 a          358 c          343 g          505 t
ORIGIN      
        1 tgacagcagt tctgccgagt ggtttcttta cggatggatg gtatttactg ttctcggaat
       61 tctgaacatt ctctatcatc ttcactatct cccatataat ataattatag tatattcttt
      121 tgtgttctat gttcctctgg atttttccat cctgaacatt gcaatgttta aaagagtgaa
      181 gtcaattgaa cttgagaata ttctcaatca ggaaaaggca gacatactcg aaacagaact
      241 gattaaaatc aagtcgagaa ccaaatatga aaagtctcat atcagaggct tgaacagaga
      301 aaagattgcc ggtcagctga agaatttaat ggagactgaa aaaatatttc tggatgaaga
      361 tttaagatcc ggggatctgg ctgcaatgct tcggctgaca ccccaccagt tttcagaatt
      421 tctgagtgaa gtgatggaaa cgagttttca cagactgatt caggaataca gaatcaagga
      481 agcaatgatt cttctcagac aggacaaaaa caggaatgtg ctgaacatcg gccttgatgt
      541 aggttttcag tccaaatctg cattcaattc tgcatttaag aaaattacag ggatgactcc
      601 gatgcagtat aagaatcaga ctgctgactc aagtgcatcc tgacagaaag gatatgcctg
      661 cacagccctc ttgacgtttc atggtcagta taccatttgc aggtgcatct cggcttattg
      721 ttctgaatca gcaccctgtg cacaacgtcg cctgaacctt tgacttctgc ctcgatcaga
      781 tgttctgtcc ggttcagtat tctgacctca tctctttcag ctgctttttt tgcttttaca
      841 agacgaggat tgaatgaaag aagacggctg agtttaaaag gaagtcttct gtaataatgc
      901 tttctgctgt ccaagtcaaa tcccagaaga cctatggaat aaagtgaagc cagagcgttt
      961 atcatcattt cctgactgag actgttttcc agaacaagtt ctttcggatt gaatgtttca
     1021 tttgttctga aaaggctgct gattccgatg atccattctt ccgggatttc ttctgtcaga
     1081 ttttccagat tctttccttc accggaaaac cctctgtaaa tccctgcaga aaatacaaac
     1141 agcatattca catctttaaa atgaacattg aatgccgaac actgttcttc cggatctctg
     1201 taaaccgaga ctgattcaat gaacggcaga agtgttctga tcagattcag tctgtgaact
     1261 cctcctaatc tgacagagtt ttctttttcg attggggata aaaaatatcc atttgtattc
     1321 tctgtgagaa aatagtcaga tttgactgca cctgccggaa gtgccttgaa aagctcgaca
     1381 gcctgaatcc tggtcatctg aaatgcagat tccatctgag aaagatagta ctgaacattg
     1441 ccgagacccc gtatccactt gtcgggcaga ctgactttct tttctgttac agaacctgca
     1501 ggagtagtaa ttttcagatg atcgtgtcct acccctaaga tcatagtttc tgaacggttt
     1561 accatattga aggcccgaat gatcggatca ttaaaatcta cattggtgca tcccgaagcc
     1621 agaaattctc cgtccactgc atccttccga acatccactc tggatcgaaa aagtcggcgg
     1681 cggtgtaatg gt
//
LOCUS       DAOQGJ010000984         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51930_length_1692_cov_2.524130, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000984 DAOQGJ010000000
VERSION     DAOQGJ010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51930_length_1692_cov_2.524130"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1692)
                     /locus_tag="PK683_14290"
     CDS             complement(<1..>1692)
                     /locus_tag="PK683_14290"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH09666.1"
                     /translation="IASFIYMFSRSGVPFEYGLDSILVYRAELMSVAMIGPLLGAFLD
                     WLLLGRLTLFFRHYFKVCTVFFVLFSFLPPEYLQYILKVWQVCALTAMGFYFHTILLS
                     IRNRSSNARRLFFGLAVLILCTVFDVLDSMIFKTGINLNRFAFFAFVIGIAGVLANRF
                     LNLHEMIEDLNKNLEHKVQKRTEELQESLTEVRLLKEQQDGDYFLTTLIVSPLATNTV
                     HTERIHIDVIMKQKKKFMFKGREHEIGGDICISDQIVLKGKKYAVFINGDAMGKSIQG
                     AGGALVLGVVFKSIISRTVLFAGNQNVYPERWLKLSFIELQKIFESFDGSMMISVVMG
                     MIDTETGFMYFLNAEHPWTAVYRDEKASFIESSLKLRKIGMPGISEKSRVEIQTFQLY
                     NGDSIFIGSDGRDDLNMGTDEAGRRLINEDENLFLRTVEESSGDLEKIYRMTEEKGEV
                     TDDFSIMRIRCFGIREKSSPAEREEMIKAVQLYNGEQYDESFAVLQNSSLFNERNYHK
                     LCANIQYRRKNFREASEHYIRFLETNPEESSYLFAAAKSLKLSGDLEAAADFGERL"
BASE COUNT          474 a          400 c          305 g          513 t
ORIGIN      
        1 agtctttcac cgaagtctgc agccgcttcg agatctcctg aaagcttcag gcttttggct
       61 gctgcaaaaa ggtaagaaga ttcttcagga ttagtctcca gaaatctgat atagtgttcc
      121 gaagcttctc tgaaattttt ccttctgtac tgaatattcg cgcagagttt atgataattt
      181 ctttcattga aaagactgct gttctgcagt acagcaaatg attcatcgta ctgctctcca
      241 ttgtaaagct gaacagcctt aatcatttcc tctctttcgg caggagagga tttttctctg
      301 atcccgaagc atcgaattct cataatgctg aaatcatcag tcacttcccc cttttcttcc
      361 gtcatacggt aaatcttttc cagatcgccg gaagactctt ccactgttct cagaaaaaga
      421 ttctcatctt cattaatcag tcttcttcct gcttcatctg ttcccatatt cagatcatct
      481 cttccgtctg atccgataaa aatggaatca ccgttgtaca gctgaaacgt ctgtatctcc
      541 actctgctct tctcactgat tccaggcatt cctatttttc ggagcttaag tgaactttca
      601 atgaaggatg ctttttcatc cctgtacact gcagtccagg gatgttctgc attcagaaaa
      661 tacataaatc ctgtttctgt atcaatcatt cccattacga ctgaaatcat catcgacccg
      721 tcgaaagatt caaaaatttt ctgcagttct atgaaggaga gtttcagcca tctctccgga
      781 tagacattct gatttcctgc aaaaagcact gttctcgata ttatggattt gaacacaact
      841 cccagcacaa gagctccccc ggcgccctga atggattttc ccattgcatc accgtttata
      901 aatacagcat atttttttcc tttcagaaca atctgatcgg aaatgcagat atcccctcca
      961 atctcatgtt ctcttccctt gaacatgaat ttcttcttct gtttcatgat gacatctatg
     1021 tgaattcttt ctgtgtggac tgtgtttgtg gcaagaggac ttacaatcag tgtcgtaaga
     1081 aaataatccc catcctgctg ctctttcaaa agtctgactt ctgtgagtga ttcctgcagt
     1141 tcttctgttc ttttctgcac cttatgttcc agatttttat tcagatcctc aatcatttca
     1201 tgaagattca ggaaccggtt tgccagaact cctgctattc ctatgacgaa agcgaaaaat
     1261 gcaaatctgt tcagattgat tccagtcttg aaaatcattg aatccagaac atcgaacact
     1321 gtgcagagaa tcagtactgc cagtccaaag aaaagccttc tggcattgct gcttctgttt
     1381 cggatggaga gcagtattgt atggaaataa aaacccatag cagtgagggc gcagacctgc
     1441 cacactttca gaatgtactg aagatactca ggcggaagaa aggaaaacag aacaaaaaaa
     1501 acagtgcaga ccttgaagta gtgtctgaaa aagagagtaa gccttcccag aagaagccag
     1561 tccagaaaag ctcccagcag agggcctatc attgcaacgg acatcagctc cgctctgtac
     1621 acaagaattg aatcaagtcc gtattcaaat ggaacaccgc ttctcgaaaa catataaatg
     1681 aatgaggcta tt
//
LOCUS       DAOQGJ010000985         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_51982_length_1691_cov_2.742054, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000985 DAOQGJ010000000
VERSION     DAOQGJ010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51982_length_1691_cov_2.742054"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PK683_14295"
     CDS             complement(<1..474)
                     /locus_tag="PK683_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09667.1"
                     /translation="MKIKYILFMLFSMPAFADTLVLKNGKTLENVRTRLLKEGVTVTS
                     ADGKVMNFAKTEIKSIRFRPVVVKTNDKKELEQERIRIADTLTESSEWEIDPSLKPKV
                     AVISFQAGKGVTEAEAETILELISTGFVKTKLFLVVDKLTLDQTAKELNCSPRDCS"
     gene            complement(707..>1691)
                     /locus_tag="PK683_14300"
     CDS             complement(707..>1691)
                     /locus_tag="PK683_14300"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNH09668.1"
                     /translation="DLAGLKKGISIGLGIVLTLTASAVLAVAVTGTINTFSSGNVVDA
                     SQINTNFTSLKTAIEGITSSQWMSSGSNIYYNAGAVSIGTSIPDAGLDIVTSQGMAYF
                     HSNKNNNSVKNARLGMLSYSNAPYIPLTWFTSTGSAGTNDSGISIGGGTLAGYAASTV
                     TIYTAANDTTLQGTERVRVTGTGNVGIGTTSPTALLHVAGTAGNNTGTWSNLSDERLK
                     KNIFDYKGSLEKVLNLRPVSFEWKENKEGKTAGRHIGFIAQEVEKIFPEWVSTMSDGN
                     KWMTPEGMNAVLVQSIRELKSLYDNEKSENAKLKQKLLSVEERLNALEKRK"
BASE COUNT          412 a          430 c          331 g          518 t
ORIGIN      
        1 ggaacagtct ctaggactgc agttcagttc ctttgcagtc tggtcgagag tcagcttgtc
       61 cactacaaga aaaagttttg tcttcacaaa tcctgtggag atcagttcca gaatggtctc
      121 tgcctccgct tcagtgactc ctttccctgc ctgaaaactg atcactgcga ccttgggttt
      181 cagcgaagga tcaatctccc attcggaact ttctgtcaga gtgtccgcaa tacgaatccg
      241 ttcctgttcg agttcctttt tgtcattagt cttcactaca acaggcctga acctgatgga
      301 cttgatctca gtctttgcaa agttcattac ctttccgtcc gccgatgtga cagtgactcc
      361 ttccttcaga agccttgttc tcacattctc cagcgttttc ccgtttttca gcacaagtgt
      421 gtctgcaaat gcaggcatgc tgaaaagcat aaataaaata tattttattt tcatgtgttc
      481 tcctttttta cctcacaacc tcaccccggc ttccagccac ccctctccat aacaatgaaa
      541 tgatgttatg gagaggggca gaggtggatg ctttgctgat aacaagaaag cgtctcttcg
      601 gattcccctt ctccaaaacg gaatccttgc cgttttggag aaggggccag gggatgaggt
      661 acaccgttcg ctggggctag gggaaagcgt aagcgaggca gcaaaatcat tttctcttct
      721 ccaatgcatt cagtctttct tccacagata agagcttctg tttcagcttt gcattttccg
      781 atttctcatt atcgtataat gacttcagtt cccgaattga ctgcaccaaa actgcattca
      841 ttccttcagg agtcatccac ttgttcccgt ccgacattgt agacacccat tccggaaata
      901 tcttttccac ttcctgagcg atgaaaccga tatgcctgcc cgcagtttta ccctctttgt
      961 tctctttcca ttcaaaactc acaggacgca gattcagtac tttttctaag gaacctttat
     1021 aatcaaagat gttctttttc agtctctcat cactcagatt actccaagtc cctgtattat
     1081 tccctgcagt gcctgccacg tggagaaggg cggtaggcga cgttgtaccg atcccgacgt
     1141 tacctgttcc agtgacacgg actctttccg taccttgtaa tgtagtatca tttgctgctg
     1201 tatagatagt cactgtactt gctgcataac cagccagtgt gccaccacca atactaattc
     1261 ctgaatcatt cgttccagct gaacctgttg atgtaaacca agtcagagga atatatggag
     1321 cattcgaata tgagagcata cctaatcgag cattttttac actgttattg ttcttattag
     1381 aatgaaaata tgccattcct tgtgaagtaa caatatccaa ccctgcatca ggaatacttg
     1441 taccaatact tactgctcct gcattataat atatattact cccgctgctc atccactgcg
     1501 aacttgtgat cccttctatc gcagtcttca aactcgtaaa atttgtattg atctgggatg
     1561 catccacaac attgcccgaa gaaaatgtat tgatggttcc tgtcactgcg actgcaagaa
     1621 ctgcggatgc tgtgagtgtg agaacgatgc ccaggccgat ggatatgcct tttttcagtc
     1681 ctgcgaggtc g
//
LOCUS       DAOQGJ010000986         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52016_length_1690_cov_3.985321, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000986 DAOQGJ010000000
VERSION     DAOQGJ010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52016_length_1690_cov_3.985321"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PK683_14305"
     CDS             <1..319
                     /locus_tag="PK683_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002177678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09669.1"
                     /translation="MSVKGQPYWDIRIGIHKGPVAAGVIGRSKFSFDVWGDTVNTASR
                     LESSGVPGKVNISESVKTDVSGQFNLEYRGEIHAKSKGALPMYFAEPAKKNSLQTVQQ
                     GLR"
     gene            324..905
                     /locus_tag="PK683_14310"
     CDS             324..905
                     /locus_tag="PK683_14310"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH09670.1"
                     /translation="MITFLIPVSAVPADDEKFAEICRNNPESRVERTSKGDIIIMSPT
                     GGETGRWNMKLGFYFESWNRQCKKGIFFDSSTAFVLPSSAIRSPDIAFVETDRWNSLS
                     SENKSAFPPLCPDFILELKSKSDVLSVLQSKMTEWMENGCRLGWLIDMENKTYYIYRK
                     NSAIEKIQGESAVLYGKDVLPDFKLSMADIYGL"
     gene            1115..>1690
                     /locus_tag="PK683_14315"
     CDS             1115..>1690
                     /locus_tag="PK683_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH09671.1"
                     /translation="MTETHIVIAGAGFAGLQAVKKFGNVKGIKVTLIDRTNHHLFQPL
                     LYQVASSVLSPADIAIPIRSLTTDMKNVQVVMGEIIDINKEHKKVSLAHTTISYDYLI
                     LAMGARTSYFGHSEWEKYTVGLKNLSDALKIRSKILISFEKAELEANPERARALMSYT
                     VIGGGPTGVEMAGAIAELSHQIIRSDFRFIDP"
BASE COUNT          506 a          312 c          397 g          475 t
ORIGIN      
        1 gatgtctgtc aaggggcagc cttactggga tattcggatt ggaattcata aaggaccggt
       61 tgctgcagga gtcatcggca gatccaagtt ttcatttgat gtctggggag atacagtcaa
      121 tactgccagc cgtcttgagt catcaggtgt tcccggaaaa gtcaatatat ccgagtctgt
      181 aaagacagac gtttcaggac agtttaatct ggaatacaga ggagaaattc acgcaaaaag
      241 caaaggtgca ctgccgatgt attttgcaga accggccaag aaaaattctt tacagactgt
      301 acagcaggga ttacgttaaa accatgatta cttttctgat tccagtcagt gcggtgcctg
      361 cagatgatga gaaatttgct gaaatctgca gaaacaaccc ggagagcagg gtggaaagaa
      421 catcaaaggg agatattatc attatgtcac cgacaggcgg agaaacaggc agatggaata
      481 tgaaactggg tttttatttt gaatcctgga atcgtcaatg taagaaaggt attttcttcg
      541 attcttccac tgcatttgta cttccaagtt ccgctattcg gtctcccgac attgcctttg
      601 tagaaacaga tagatggaac tcgctcagtt ctgagaataa atcggccttt ccgccgcttt
      661 gccctgattt tattctggaa ctcaaatcaa agtcagatgt attgtctgtt cttcagtcaa
      721 aaatgaccga atggatggaa aacggatgtc gtctcggatg gctgattgat atggaaaata
      781 aaacatatta tatatacaga aaaaattctg caatagaaaa aattcaggga gaatctgctg
      841 ttctttatgg aaaagacgtt ctgcctgatt ttaagctgag tatggcggat atttatggat
      901 tatagaacag tcatttataa aggaataaaa aatcggtctg attgactgta ttattggtga
      961 cagggttgtc tgctgtcaca gatctggatt cggccctttg caatttctga acagacagta
     1021 tttctcttct ttttcgccgg acacattttt cgtgtatatg aaaaaattat tgaaagccga
     1081 agtcatctgc agatcctgaa tccagaggga taagatgaca gagactcata ttgtgattgc
     1141 gggtgcagga tttgccggtc ttcaggcagt gaaaaaattc ggaaatgtaa agggaatcaa
     1201 ggtgactctg attgacagaa cgaatcatca tctttttcag cctcttctct atcaggtggc
     1261 cagttctgtc ttaagtccag cagacattgc cattcccatc aggtcactta caactgatat
     1321 gaaaaacgtg caagttgtaa tgggagagat catagacatt aataaagaac acaagaaagt
     1381 aagccttgct catactacaa tttcttatga ttatctgatc ctggcaatgg gggcacgtac
     1441 aagttatttc ggtcacagtg aatgggaaaa atatacagtc ggtcttaaaa acttgagtga
     1501 tgcactgaag atccgttcga agattttgat cagtttcgaa aaagcggaat tggaagccaa
     1561 tcctgaacgt gcgcgggcac tgatgagtta cactgtgatc ggaggaggtc ctaccggtgt
     1621 ggagatggca ggtgcgattg cagaactttc ccatcagatc atccggagcg atttccgttt
     1681 cattgatcct
//
LOCUS       DAOQGJ010000987         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52034_length_1690_cov_3.018960, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000987 DAOQGJ010000000
VERSION     DAOQGJ010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52034_length_1690_cov_3.018960"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PK683_14320"
     CDS             complement(<1..826)
                     /locus_tag="PK683_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNH09672.1"
                     /translation="MIYRLKRFDGEWRFILDNGVPRTDENGTFAGFIGSCIDITERLK
                     TENALRESEEKFRTLVNSSEDIIFTLDTEEKHTGVFGKWMEKQNIPETFFLGKTVSEI
                     FGAEEGKIHSDANKKALSGENAVYSWSVQGNDKTRYYQTSLSPMHGKDGSVAGLVGIG
                     REITDLKESEEKMQEINRRLESAVKKSMELAAQAESANQAKSEFLAMMSHEIRTPMNG
                     VIGMTGLLLDTELNEEQKQFAEIVKSSADSLLNLINDILDFSKIEAKKLELESLDFN"
     gene            complement(971..1351)
                     /locus_tag="PK683_14325"
     CDS             complement(971..1351)
                     /locus_tag="PK683_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09673.1"
                     /translation="MNKIQKIVYSFMILFGILTVFSGGKHLLLWNELQSEGKNFHLWI
                     LTFNFIAGFFYILAGIGLLREKHWSRILVYGIASATAVFYAVFIILVLNGSPYEKQTL
                     YAMGFRTIFWIGAAVMVRWRGIPA"
BASE COUNT          435 a          381 c          310 g          564 t
ORIGIN      
        1 gattaaaatc cagagattcc agttccagct tctttgcttc tattttggaa aaatcaagta
       61 tgtcattgat cagattcaga agagagtctg cacttgattt gactatttcc gcaaactgtt
      121 tctgttcctc attcagttct gtatccagaa gaagtcctgt cattccgatc acaccgttca
      181 tcggtgttct gatctcatgg ctcatcattg ccaggaattc actttttgcc tgattggctg
      241 attcagcctg agcagcgagc tccattgatt tttttactgc tgattccagt ctcctgttga
      301 tttcctgcat tttttcttct gattctttca gatcagtgat ctctcttcca atccctacca
      361 gacctgccac tgagccgtcc tttccatgca taggcgataa tgatgtctga taataacgag
      421 ttttatcatt tccctgtaca ctccagctgt agactgcgtt ttctcctgaa agagcttttt
      481 tatttgcatc agaatgaatt ttcccttctt ctgctccgaa gatctctgag actgtttttc
      541 caagaaagaa tgtttcagga atattctgct tttccatcca tttcccgaat actcctgtat
      601 gcttttcttc cgtgtcgaga gtaaatatga tgtcttcgga agaattgact aaggttctga
      661 atttttcctc actctctctg agcgcatttt ctgttttcag tctttcggta atatcaatgc
      721 aggaaccgat gaaaccggca aatgttccgt tctcatcagt tctgggcacg ccgttgtcca
      781 gtataaatct ccattcaccg tcaaatctct tcagtctgta tatcatactg aatgcttctc
      841 ttctgtcaaa atgatgtaca tatgtatcgt ctctcgtgat tttataaact tcttctataa
      901 atattgattc taactcgctt taggacaaaa tggccaacct gtaaagattt tttattttac
      961 caataaaaaa ttacgcagga atccctctcc acctcaccat cactgcggca ccgatccaga
     1021 atattgttct gaatcccatt gcgtacaatg tctgtttttc gtaaggagaa ccgttgagga
     1081 ctaggataat aaatactgca tagaatactg ctgttgcaga ggcgataccg tagacaagga
     1141 ttcggctcca gtgtttttcc cggagcaggc cgattcccgc gaggatgtaa aaaaaacctg
     1201 caatgaaatt gaatgtcaga atccagagat gaaaattttt gccttctgac tgaagttcgt
     1261 tccagaggag aagatgtttt cctcctgaaa agactgtcag tattccgaag aggatcataa
     1321 aggaatatac gattttttga attttattca tagggatagt ttttattctg aagatgccct
     1381 ggcaatcgag ttacttcttc tttagatctg cagcttcgcg gtttctctta agatgatgca
     1441 taaaagcata tttgccaatt atccaggcga tgaacacctg ctttgctgtc ttgcacgcag
     1501 ttacggaaac ggtgaacgga atcatgaagg aagaatttgc acttgacctt gttttttcag
     1561 atctgaaaga aatcttcaga cgccatcagg cagcataaaa aagtgacaac ctattcccgg
     1621 cttcgctatc cgctattccc cttgacttcc ctttgccccc ggataaacta ttcaattatg
     1681 tttgccgaat
//
LOCUS       DAOQGJ010000988         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52052_length_1690_cov_2.477676, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000988 DAOQGJ010000000
VERSION     DAOQGJ010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52052_length_1690_cov_2.477676"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1690)
                     /locus_tag="PK683_14330"
     CDS             complement(<1..>1690)
                     /locus_tag="PK683_14330"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09674.1"
                     /translation="QVDSVSYEYDSRGNPERVVKFPGSEAENASVYSYDSYGNTASFT
                     NALGRVTYYSYDSDIHSYPVSVKNPLGHTELKTYNTVSGAELVSTDANGASVEKTYDE
                     FGRLETVKFPELSEETERYVYQLTDSPKGAKTVKFVNDPDNGVSVSTEYFDVEGRTVR
                     KESPLMGGLVQAETMEYDLLGRLVRKSSPYVEGKTNPKYSLYEYDADSVLQKETHPDG
                     SYTVTQESGFNVSAVTASASGAVMSEEILEKNETGQVLKRTVQGKTLSYKYDPAGRFS
                     EISDPENGKTSILYDTAGRKKLQTDENSGTVSYTYDSIGSLKTQKDARGVILTYQYDA
                     LNRVERIDSSKGEPPVLFSYDSGSNALGRMSRVQDETGEAHFEYDLRGNMISALKKTD
                     ELNVVFRKFYDVLGRLKTSVYPDGTKTHSRYSQSGHLYEITMDSADGQSTGHSLIRYG
                     IEVPDGESLELVRTAGNGTETRIRFDELKKRPLSLAARLNGGHLEQMLSYSYDEKGNI
                     TAVEDKIIEGRTQRFEYDSLNRITKASGRYGTEEYRYSEGGNLLQRGAFALKYND"
BASE COUNT          400 a          424 c          325 g          541 t
ORIGIN      
        1 gtcattatat tttaatgcaa atgcacctct ctgcagaaga ttgccccctt ccgaatacct
       61 gtattcctct gttccgtacc tgccggatgc ctttgtgatt ctgttcaggc tgtcatattc
      121 aaacctctgt gttctgccct caataatttt gtcttccact gcagtgatat ttcctttttc
      181 atcatacgaa taggacagca tctgttcgag atgtcctccg ttcagacgtg ctgcaagaga
      241 cagcggcctt ttcttcagct catcaaaccg aatccgtgtc tctgttccgt ttcctgctgt
      301 tctgacaagc tccaggctct cgccgtcagg cacttcaatc ccgtaccgga taagactgtg
      361 acctgtgctc tgaccgtctg cactgtccat agtaatttca tagagatgtc cgctctgcga
      421 atacctgctg tgtgtttttg tcccgtcagg gtaaacagaa gttttcagcc ttcccaatac
      481 atcataaaac ttcctgaaca ccacattcag ttcatctgtc ttcttcagtg cagagatcat
      541 atttccccgg agatcgtatt caaaatgagc ctcaccggtt tcatcctgaa ccctgctcat
      601 tcttccgagg gcatttgaac cggagtcata ggaaaacagt acgggcggtt ctcctttact
      661 gctgtctatt ctttccaccc ggttcagagc atcatactga tacgtcagaa taactcctct
      721 tgcatctttc tgagttttca gactgcctat ggaatcgtaa gtgtaactga ctgtccctga
      781 gttttcatct gtctgcaatt tttttcttcc tgctgtatca tagagaattg aagtttttcc
      841 gttttcagga tctgaaattt cagaaaacct tcctgcagga tcgtatttat aagaaagagt
      901 cttcccctgc actgttcttt tcagcacctg tcctgtttca tttttttcca gaatttcttc
      961 cgacataacg gcaccagaag cagaggccgt tacagcagat acattgaaac ctgattcctg
     1021 tgttacagta taacttccgt caggatgagt ttctttttga agaacgctgt ctgcatcata
     1081 ttcatagagg gaatatttcg ggtttgtctt tccttccaca tacggggaag attttctgac
     1141 aagtcttccg agcagatcat attccattgt ctctgcctga accagtccgc ccataagagg
     1201 agattccttt ctgacagttc ttccttccac atcaaagtat tccgtgctga cagatacccc
     1261 attgtcagga tcatttacaa atttgacagt cttggcccct ttcggggaat cagtcaactg
     1321 atacacatat ctttctgttt cctcagacag ttccggaaat ttcactgtct ccagccgtcc
     1381 gaattcatca taggtctttt ccacggaagc tccgtttgca tctgttgaaa caagctcagc
     1441 tccggaaact gtattgtatg ttttcagttc ggtatgaccg agaggatttt tcacggatac
     1501 aggataggaa tgaatgtctg aatcgtatga atagtaagtg actctgccga gggcattcgt
     1561 aaatgatgca gtattcccgt aactgtcata agaatagaca gatgcatttt ctgcttcaga
     1621 tccagggaac ttcacgacac gttcaggatt tcctctgctg tcatactcat aagaaacaga
     1681 atcgacctgt
//
LOCUS       DAOQGJ010000989         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52097_length_1689_cov_2.756426, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000989 DAOQGJ010000000
VERSION     DAOQGJ010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52097_length_1689_cov_2.756426"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PK683_14335"
     CDS             <1..623
                     /locus_tag="PK683_14335"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNH09675.1"
                     /translation="NSTTCAITGTPTARKTSAQYTITASGSAGSASAAITLAVIPSAF
                     LKIFSTAGSLGPNLVGANADSTCMADGNKPGGGGTYKAMAALTTRRACTTANCSGGAS
                     ENFNWILYPNTQYRRGDGVTVIGTTTASAIFTFNLTNSFTSSAVFPSTFLNTDWTQNA
                     TENCSDGTSTAGNVRFAQGVSTTNGSISFNSTGCNAGGRELYCVEQ"
     gene            864..1592
                     /locus_tag="PK683_14340"
     CDS             864..1592
                     /locus_tag="PK683_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNH09676.1"
                     /translation="MKNLKFLIVLTALLFCKNDKNSYNSLISQVLGLSPQYWPAIQTI
                     EPRKGSPQSQTATGVNSATNVIIKGRNFSFIPSENIITFNGIAAPVNYSTDKEIWTAV
                     PNGATSGLLSVSKPGGICMSLDKKSGPGCAASEFYIDCYAPYNRLYGEETLLKVGTIT
                     KITYDSFETKPFRVELLDGDHTVSISCKSPVSVRRFTNTCTASEETQNGSTVLINPQL
                     KLTGGYTFQFFLSSTKSECSILAL"
BASE COUNT          512 a          369 c          332 g          476 t
ORIGIN      
        1 taaacagtac aacctgtgca attaccggga cacctactgc aagaaaaaca tctgctcagt
       61 acacaattac tgcttcaggt tctgcgggaa gtgcatcagc cgcaattaca ctcgcagtta
      121 ttccttctgc cttcctgaag attttctcta ctgcaggttc ccttggaccg aacctggtgg
      181 gagcgaatgc agattccacc tgtatggctg acggaaacaa acctggagga ggaggtacat
      241 ataaggcgat ggcagcattg acgacaaggc gagcatgcac tactgcaaac tgttcaggcg
      301 gtgcttcaga gaatttcaac tggattcttt atcccaatac acagtaccgg agaggtgacg
      361 gcgtgactgt cataggaaca acgacggcct cagccatttt tacttttaat cttacaaatt
      421 cttttacttc atcagcagtt tttccgagta ccttcttaaa tacggactgg acacaaaatg
      481 ccacagaaaa ctgttcagac ggcacttcca ctgcaggtaa tgtccgtttt gcacaaggtg
      541 tttccacaac aaacggctca atcagcttca attcaactgg ctgcaatgcc ggcggacgtg
      601 agctttactg cgtggagcag tgacgggaaa aattacactt cattacttca gtcatcgatt
      661 ttttcgtaat ttttactggc ttatactcat attcaaaagt ctttgcgtat ttctacttta
      721 gtgagccggc aaaatcaaga ttttcgaatg taaaaagcgc aattactttt ggtaatcagt
      781 ataatttctc atcgtgcttt ttcactgaag ggttataaaa tgtgcttcca taaagaagca
      841 aagtctgtac aattgaaatc ctgatgaaaa atctgaagtt cctgattgtt ttgactgctc
      901 ttctcttctg caaaaatgat aagaattctt acaacagtct gatttctcag gtattgggtc
      961 tttctcctca atattggcct gctattcaga caatagagcc ccgaaaaggt tcacctcaat
     1021 cgcagacagc tacgggtgta aattctgcga ctaacgttat tataaaggga agaaatttct
     1081 cattcatccc ttcagaaaac ataattactt tcaacggaat tgctgcacct gtaaactatt
     1141 ccacagacaa ggaaatatgg actgctgttc caaatggagc aacgagtgga cttctctcag
     1201 tttccaaacc cggaggaatc tgtatgagtc tagacaaaaa atcaggtccg ggatgcgctg
     1261 catcagagtt ttatattgac tgttatgccc catacaacag attatacgga gaagaaactc
     1321 ttctcaaagt cggcacaatt actaaaatca catatgattc atttgaaact aagccgtttc
     1381 gtgtagagct tctggacgga gatcatacgg tcagtatttc gtgcaagtca cccgtctctg
     1441 tgcgaaggtt tacaaatacc tgcactgcgt ccgaagaaac tcagaacggt tccactgtcc
     1501 tgatcaaccc ccagttaaaa ttgacaggtg ggtatacctt tcagttcttt ctttccagca
     1561 caaaaagtga atgcagtatt ctggctcttt gattaagaag aaatttacaa aaaaccatat
     1621 taagagaaaa atgaaattaa actgcttcaa acagctcggt aatatattca atagaagaaa
     1681 ctagaatat
//
LOCUS       DAOQGJ010000990         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52106_length_1689_cov_2.236230, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000990 DAOQGJ010000000
VERSION     DAOQGJ010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52106_length_1689_cov_2.236230"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1689)
                     /locus_tag="PK683_14345"
     CDS             complement(<1..>1689)
                     /locus_tag="PK683_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNH09677.1"
                     /translation="LKSLSLFSDWTDEINVREEKYIQLKALNQLEEYLKNPENETDFY
                     DKLNLLIELTDSTEKKIISADKYQILNRVFFTEFQNEMKTVLRGYDALTERISSLCTG
                     ILNTVLIIANHMHAGDGNVHVNIPVLSNDLDMMRRAEHLVDEVMAKTLELGGAVSGEH
                     GIGITKIKYLDDSAVLKMKNYLQKADPSGLMNPEKLVSKSVINRVFTPSFNLLELEAR
                     ILKYGNLETLAGKIAHCVRCGKCKTDCCVFHPAQNMFFHPRNKNLAIGSLIEAVLFEV
                     QRYRNVSFELLGRLEEIADNCTVCHKCKIPCPVKIDTGEITVLERNLLVQRGIKKTPS
                     IANLTLKYLSSTSPVYNRLIHTAVLGFGVRLQNVSSRAVSPLQSFRKISNNYTMMILQ
                     VPSVKSSFKVLYDFLPRCESNQILMFEGDSHPEFTYFYFPGCGSERLFSEISLAGLYL
                     LLKSKARVILPPPFLCCGFPFGVNGRQEELSKIVLRNMIIFSQIKDMFKYIKFDAVCV
                     TCGTCRESLERTGTADIFETKITDVLNVIPLNLKTEDKVLYHKPCHDSLDSRGVE"
BASE COUNT          473 a          348 c          341 g          527 t
ORIGIN      
        1 ttcaactcct ctgctgtcga gggaatcgtg gcagggctta tggtacaata ctttatcctc
       61 tgtttttaaa ttcaacggaa tgacgttcaa tacatcggta attttagttt caaagatatc
      121 agctgtgcct gtgcgttcaa gagactctct gcatgttccg caggtgacgc atacagcatc
      181 gaactttata tatttgaaca tatctttgat ctggctgaaa ataatcatat ttctcaatac
      241 gattttagac agttcctcct gtctgccgtt tacaccgaac ggaaaaccgc agcataggaa
      301 agggggagga agtattactc tggcctttga tttcaggaga agataaagac ctgccagcga
      361 gatttccgag aacagtcttt ctgaaccgca tccgggaaaa taaaaataag tgaattcagg
      421 atgactgtct ccttcaaaca tcagtatctg attcgattcg catctgggca gaaaatcata
      481 gagaacttta aatgaagatt ttactgaagg gacctgcagt atcatcatcg tataattatt
      541 ggatatcttt ctaaaggact gaagaggcga aactgcccgg cttgaaacat tctgaagcct
      601 taccccgaat ccgagaacgg ctgtatgaat cagtctgtta tagacaggag aggtagatga
      661 aagatatttc agagtcagat ttgcaatgga aggagttttc ttgattcctc tctgcacaag
      721 caggtttctt tccaatacag ttatttcccc agtatctatt tttacaggac acggaatttt
      781 gcatttatgg catacagtac agttgtctgc gatctcttcc agacggccaa gaagttcaaa
      841 tgaaacgttt ctgtatctct gcacttcaaa cagaacagct tcaatcagtg agccgatcgc
      901 cagattttta tttctagggt gaaaaaacat attctgcgca ggatgaaaaa cgcagcagtc
      961 cgtcttgcat tttccgcatc ggacacagtg ggctattttt ccagcaagag tttccagatt
     1021 cccgtatttg agaatcctgg cttcaagttc aagaaggttg aaggatgggg taaacactct
     1081 gtttattact gatttgctga caagtttttc cgggttcatc agtcccgaag gatcagcttt
     1141 ctgcagatag tttttcattt tcaagacggc agaatcatcc agatacttta tctttgtaat
     1201 tccgatcccg tgttcgccgc ttacagcacc tccaagttcc agtgtttttg ccataacctc
     1261 atctaccaga tgttcagcgc gtctcatcat atccagatcg ttggaaagaa ccggaatatt
     1321 gacatgtacg ttcccgtcgc ctgcatgcat atgatttgca atgatcagaa cagtattcag
     1381 aattccggtg cagagagagc ttatcctttc tgtcagagca tcatatcctc tcagaacggt
     1441 tttcatttca ttctgaaatt cagtaaaaaa gactctgttc agaatttgat atttgtctgc
     1501 cgagataatt tttttttctg tactgtctgt gagctcaatt agaagattca gtttatcata
     1561 aaaatcggtt tcattttcgg gattttttaa atactcttca agctggttca atgctttcag
     1621 ctgaatgtat ttttcttcgc gaacattgat ttcatctgtc cagtccgaaa aaagagaaag
     1681 agatttcag
//
LOCUS       DAOQGJ010000991         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52172_length_1687_cov_4.261029, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000991 DAOQGJ010000000
VERSION     DAOQGJ010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52172_length_1687_cov_4.261029"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1687
                     /locus_tag="PK683_14350"
     CDS             <1..>1687
                     /locus_tag="PK683_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09678.1"
                     /translation="ELYYDILRDHGVLTASENTISYEEEHRLEDAKAVINDLNKNGIG
                     ITAALQQAVSKIPDGPDAEKQRRLLIDAAVQMQISNVTDLNKMKDLLTANENSGGLYE
                     PEDLKKVAEALLMKQKTLADVMTVGPALEKLLWNSGADTSEFWKTATATALKKNESGS
                     PIPKDFISSFVFRDMPEDAKAGLQNIAVNAELKQDFSKQLSNLKKQNLDAFLAGYDKL
                     NSVALERADRVPTAGSDLDKALADKAGASLENTMKLKGTDAAKLAKEKILASLGNSPS
                     AQAAVETRFKDVEQNYLKEKAVSEMLKAKSGLELADRYNDVMARENLDPGTKQALKES
                     FSTISGNVLYSLYKTGGTEAAEKFINEIRNSSQDMHTDIGIKMQEKLGKLLIENSAKT
                     GEGTLQNLNEAVNRLKNDTDFKNLSPSEQADAVNRLTSAAEDRILSMARETAGKGDSD
                     TNVNAVKELRDAMKNSSEYKFSEQKINELENVFKKAEARNMILTKFDSFANLDSKRID
                     TIKLEETKKIAAEFLVADQLKKNRELLIDSLKSLPKEDADIRDRVAKNEQNERK"
BASE COUNT          591 a          305 c          411 g          380 t
ORIGIN      
        1 gaactctatt atgatatttt aagggatcat ggagttctta ctgcttcaga aaatacgata
       61 tcttatgaag aagaacatcg tctggaggat gcaaaagcgg taattaatga tctcaataaa
      121 aacggcatag gcatcactgc ggcacttcag caggctgttt ctaaaatccc tgatggtcca
      181 gatgcagaaa aacagagaag acttttgatt gatgctgctg tacagatgca gatttccaat
      241 gtcactgatc tgaataaaat gaaagacctt cttactgcaa atgagaacag tggaggtcta
      301 tatgaaccgg aagatttaaa gaaggttgcc gaagctcttc tgatgaaaca gaaaactttg
      361 gcagatgtaa tgactgtcgg accggcattg gaaaaacttc tctggaattc gggtgctgac
      421 acgtctgagt tctggaagac tgctacagcg actgccctta aaaaaaatga atccggcagc
      481 ccgattccga aagactttat aagcagtttt gtatttcgtg atatgccgga ggatgcaaaa
      541 gcaggtcttc agaatattgc tgtaaatgct gaactcaaac aggatttttc aaagcagctc
      601 tctaatttga aaaagcaaaa cctggatgct tttcttgcag gctatgacaa actgaattct
      661 gttgcactgg aaagagcaga cagggttccg actgcgggga gcgatctgga taaggcactt
      721 gctgacaagg caggggcttc tttagagaac acgatgaagc tgaaaggaac tgatgctgcg
      781 aagcttgcaa aagaaaaaat tcttgctagt ctaggcaata gtccttctgc acaggctgca
      841 gtagagacca ggttcaagga tgtagagcag aattatctga aagagaaagc tgtcagtgaa
      901 atgctgaaag caaaaagcgg actggagctt gcagacaggt ataatgatgt gatggcaaga
      961 gagaatcttg atcccggaac aaaacaggca cttaaggaaa gcttcagcac aatctcgggc
     1021 aacgttcttt acagtcttta taaaacggga ggcactgaag ctgcagagaa atttatcaat
     1081 gagataagaa acagcagcca ggatatgcat accgacatcg gcatcaagat gcaggaaaaa
     1141 ctgggaaaac ttctgataga gaactctgcg aagacaggag aaggcacgct gcagaatctc
     1201 aatgaagcag tgaatcgtct gaaaaacgac actgacttca aaaacctgag tccaagtgaa
     1261 caggcggacg ctgtaaacag actgacgtct gccgcagaag acaggattct cagtatggcc
     1321 agagagacag caggaaaagg agacagtgat acaaatgtga atgctgtcaa agagctgcgg
     1381 gatgcaatga aaaattcttc tgaatacaaa ttttctgaac agaagatcaa tgaactcgaa
     1441 aatgttttca agaaggcaga ggcgagaaac atgatactca caaaatttga cagttttgcg
     1501 aatctcgatt caaaaagaat cgacactata aaactggaag agacaaaaaa gattgctgcg
     1561 gaattcctgg ttgcggatca gttgaaaaaa aacagagaac ttctgattga ttcgttaaaa
     1621 tctcttccaa aagaagatgc agatattaga gacagggttg cgaaaaacga acagaatgag
     1681 cgcaagg
//
LOCUS       DAOQGJ010000992         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52175_length_1687_cov_3.883578, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000992 DAOQGJ010000000
VERSION     DAOQGJ010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52175_length_1687_cov_3.883578"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="PK683_14355"
     CDS             complement(<1..1411)
                     /locus_tag="PK683_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH09679.1"
                     /translation="MDETRKKVLIIISSGCTAAGFLWSLLYFILGLKLPALPPILFSL
                     GVGSALIVYFSTGKINLLLHSQLFMILIIPAVLQWSLGGFHKSGVVILWSSLAPFGSL
                     IFQPARKSIVWVLMFFSVLAVSFYYDSDLQKFFPYSEITPSVNLLFYGMNIICTLIVS
                     FAAVYYFSSSLSGEKNRQEDYLKELSKDVDSMLHYIELLSAGNIGEKMDESSFDPIIL
                     KLKKGYNTSIDLFRNIVSDLKTNSENFLSSAENIRRSLDGIQDKISDNSQSLVYLESD
                     FGKIRADVKSISLAIRESVSSSENNLDYARKSTLVIKETVKKLEEISFSVKETNEQIS
                     NLEKNAKEIDEILTGIDEISDSTNLLSLNASIEAARAGEHGRGFAIVASEIGKLTART
                     SDSTKKINEKVKEIKKGIHSSSLKFAATSSLASEGMKMTSELNGVTGRIMNLSEELQT
                     AMQKMTEKGIEQTMHIDSVW"
     gene            complement(1493..>1687)
                     /locus_tag="PK683_14360"
     CDS             complement(1493..>1687)
                     /locus_tag="PK683_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09680.1"
                     /translation="YKRLLKIAKETKAGSPEMLASHLLLLMDGIFASVRMFGTKNHSS
                     SVTNACRSLLKCYQKKDISS"
BASE COUNT          508 a          354 c          329 g          496 t
ORIGIN      
        1 tccagactga atcaatatgc attgtctgtt ctattccttt ttccgtcatc ttctgcatgg
       61 cagtctgaag ttcttcggaa agattcataa tccttccggt caccccgtta agttctgaag
      121 tcattttcat tccttcggat gcaagactgc ttgtagcggc aaatttcagt gaagaactgt
      181 gaattccttt tttaatttct ttcacttttt cattgatttt cttagtggaa tcggaggtcc
      241 gtgcagtcag tttcccaatc tctgaggcta caattgcaaa acccctgcca tgctcacctg
      301 cccttgccgc ctcaatggat gcgttcagag agaggagatt tgtgctgtcg gaaatctcgt
      361 ctattccagt gagaatctca tctatttcct ttgcattttt ctcaagattg gaaatctgct
      421 cattagtctc ctttactgaa aaggaaatct cctccagttt ctttactgtc tctttaatga
      481 caagagtgct ctttctggca tagtccagat tgttttcaga agaggaaaca ctctccctga
      541 ttgccaggga gatgctttta acatcagccc tgatttttcc gaaatcactt tccagataca
      601 caagagactg agagttgtca gaaattttat cctgtatccc gtccagcgat ctgcggatat
      661 tttctgcaga gcttaaaaag ttttcagaat tagtcttaag atcagagact atgtttcgaa
      721 acagatcaat ggaagtattg tagccctttt tcagcttgag tatgatggga tcaaaggaag
      781 actcgtccat cttttcaccg atatttcccg cagacagcag ttcgatataa tgaagcatcg
      841 aatcgacatc cttgctcagt tctttcagat aatcctcctg cctgtttttt tctccgctca
      901 aggagctgga aaaataatac acagcagcaa aggatacaat cagtgtacag ataatattca
      961 ttccatagaa taaaaggttt acagacggag tgatttctga atagggaaaa aatttctgca
     1021 gatcggaatc ataatagaag gataccgcaa gaacactgaa aaacattagg acccagacga
     1081 tgctttttct ggcaggctga aaaatcagag atccgaaagg agcaagagac gaccatagga
     1141 tgacaacgcc tgatttatga aatcctccta gcgaccattg aagaactgca ggtatgatca
     1201 gaatcataaa aagctgcgaa tgaaggagta aattgatctt tccggtggaa aaatagacaa
     1261 tcagtgcaga accgactcca agcgaaaaaa gaataggcgg aagagccgga agtttcaatc
     1321 ccagaataaa gtaaagaaga gaccacaaaa aaccggctgc agtacaccct gaactgatga
     1381 taatcagtac cttttttctt gtttcatcca taaaatcctc acaacagaaa atcagaaata
     1441 cagaataccg caactaaaaa actgtatcag gttttttttt agagaatatt tttcaggaag
     1501 atatgtcttt tttctgataa catttcagca aagatctgca tgcgttagtc acagaggaag
     1561 aatgattctt agtcccaaac attcttacgg aagcaaatat tccgtccatc agcaggagaa
     1621 ggtgagatgc gagcatttcc gggctccctg cctttgtttc ctttgctatc ttgagaagtc
     1681 ttttata
//
LOCUS       DAOQGJ010000993         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52180_length_1687_cov_3.474877, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000993 DAOQGJ010000000
VERSION     DAOQGJ010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52180_length_1687_cov_3.474877"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK683_14365"
     CDS             complement(<1..413)
                     /locus_tag="PK683_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNH09681.1"
                     /translation="MTEKEFDIVVYGATGFTGRLVAEYLTLRGFKGWAMAGRSETKLQ
                     EIRDEVGVSKDIPLIVADASNPDSLNALCEKAKIVLTTVGPYQLYGSELVAVCANTGT
                     DYLDLNGEPAWARQMIDEHEAAAKKSGARIVLSAG"
     gene            826..>1687
                     /locus_tag="PK683_14370"
     CDS             826..>1687
                     /locus_tag="PK683_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09682.1"
                     /translation="MQFLQEKDEKFEAGTNKDGISKIIYKQTDKEYSSLLYDAKGKRA
                     GEIYFRILPDGWETMQKSEAGFEKTVYNKKGSVILKENFGADGQPKADESGTAAVKYE
                     YNEKGNLLVKGTFGLNGRPAADKDGIAVWKKKYNDKGDILSEEKFDGKGVLKADSSGI
                     ARTLYKYDERGSSRLTESYGADGKLKNNEKGFARIVEKSNKNGQSELMEFYDENGRLK
                     GVIFGIARAAVQYDSKGNKILEETFGEDGKLKADNRGTARSIRKYSEKGETLSEELYG
                     ADEKLITRTVY"
BASE COUNT          573 a          320 c          380 g          414 t
ORIGIN      
        1 aatcctgcag ataaaacaat acgtgcaccg cttttcttcg cagcagcttc atgttcatca
       61 atcatctgcc gcgcccaggc cggctctccg ttcaggtcca gataatcagt tccggtattt
      121 gcacatactg caactaattc agatccgtag agctgataag gaccgactgt agtcagaaca
      181 attttcgcct tttcgcacaa tgcattgagg gaatcagggt ttgaggcatc agcgactatc
      241 agaggtatat ccttggaaac tccgacttca tctctgattt cctgaagttt tgtttcactt
      301 ctgcctgcca ttgcccagcc tttgaacccg cgcagagtaa gatactctgc aacaagcctg
      361 ccagtaaaac cagttgctcc ataaacaaca atgtcgaatt ctttctcagt catagataac
      421 ccttttcagc aaatctgctt ttctcaggat aattctgtat aaaatttatg tatataataa
      481 atccgttaat tcattcaaaa aggggtgcac ccgattctat cattaaaaaa atctttacaa
      541 ttctgttctg tataaacaat tacgttatga aaaaaattat cctctccatc ttgattccct
      601 ttgtatacct gagttccgag tccggcaaga taaaaaagac tgcagctgaa actccgaaaa
      661 aagacagctg taaccgaatc ctcggacaag gagaaacatt ccctgaaaaa agactctgaa
      721 tctgaaaagg gccagaagag ggaaggcatc cttctgaaac acctgggagg ggaagaatct
      781 gaatttggca gactttttac aggaaaggac catattcagg aagaaatgca gtttcttcag
      841 gagaaggacg aaaagtttga agccggaaca aataaagacg ggatcagtaa aattatttat
      901 aagcagacag ataaagaata ttcatcatta ttatatgatg ccaaagggaa acgtgccggc
      961 gaaatctatt tcagaatcct gcctgacgga tgggagacaa tgcaaaaatc ggaagcgggt
     1021 tttgagaaga cggtgtataa taaaaaaggt tcagttattc tgaaggaaaa tttcggagcg
     1081 gacggtcagc ctaaagcaga tgagtccgga acagctgcag tgaaatatga atacaatgaa
     1141 aaaggaaatc ttctcgtcaa aggaacattc ggtctgaacg gcagacctgc ggctgacaag
     1201 gatgggattg ccgtctggaa aaaaaaatat aatgataaag gagatatact gtcagaagaa
     1261 aaatttgatg ggaaaggggt tttaaaggca gacagttcgg gaattgcgcg taccctgtat
     1321 aagtatgatg aaagaggttc ttcaagactg actgaatcct atggagcgga tggaaaattg
     1381 aagaataacg agaaaggatt tgcacgtata gtcgaaaagt ccaataagaa cggccagtcc
     1441 gaactgatgg agttttatga tgaaaacggc agactgaagg gagttatctt tggaattgcc
     1501 cgtgctgcag ttcaatacga ttcaaaaggc aataaaatac tggaagaaac ttttggtgag
     1561 gacggaaaat taaaggccga taaccggggc acagcccgtt ccataagaaa atacagtgaa
     1621 aagggagaga cactgtcgga agaattatat ggagccgatg aaaaacttat taccaggact
     1681 gtttata
//
LOCUS       DAOQGJ010000994         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52212_length_1687_cov_2.160539, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000994 DAOQGJ010000000
VERSION     DAOQGJ010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52212_length_1687_cov_2.160539"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1687
                     /locus_tag="PK683_14375"
     CDS             <1..>1687
                     /locus_tag="PK683_14375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH09683.1"
                     /translation="VNREFEFIKRDGSTIYVLLEARPITDAQGNYDGTIAGLMDITDR
                     KKIEHALRFIAEGSWLESMENFLKSIILYISEVLGITYAMMEEILPDGKIRRIIGSAE
                     SGEVLHSEEVFSKKNINSQIIGNKYNIYHKNAQTEFPEDSLLAELQIESYAGIPLWNS
                     NRQSIGQIAVLGKEPISKPELVESILKIAAVRASHEMERIKARDELEQRIRERTEELN
                     LAKEQADSATRAKSEFLANMSHEIRTPLNAVLGFTALLARGPLTEVQKEHLKNLSSAA
                     DILLQLINDIIDFSKIEAGKLELEKAEFNLGEVCRKISSIFAFKAQEKGLELVFYLKN
                     SVPLHLIGDSLRLEELLLNILGNAVKFTEKGSIVLQIGLEEETESDALIYFSIKDTGI
                     GMTEEQRKKLFTAFTQADSSTTRKFGGTGLGLTISKKLAMLMGGDIWVQSKLGRGSTF
                     RFTVRMTKQKNPDIQTLEIPDFVRGTDVLLIEENPDAALAISEYLQALSFNAKIYLSV
                     DAAAESLKFPKGKEKQEFSMVFVESRLFSSGKRIIYSMFKDSPVKPKVTVLTGY"
BASE COUNT          571 a          339 c          386 g          391 t
ORIGIN      
        1 cgtaaacagg gaatttgaat tcatcaaaag agacggttct acgatctatg tactgctgga
       61 agcgagaccg atcacggatg cacagggaaa ttatgacgga actatagcag gtctgatgga
      121 catcacagac agaaagaaaa ttgaacatgc cctcagattc attgccgaag gaagctggct
      181 ggaaagcatg gaaaactttc tgaagtccat catactgtat atatcagaag ttcttggaat
      241 cacttatgca atgatggaag aaatccttcc agacggaaaa atcagaagaa tcatcggatc
      301 ggctgaatca ggggaagtcc ttcactcgga agaagttttt tcaaagaaaa acattaacag
      361 tcaaatcatc ggaaataaat acaatatata ccacaaaaac gcacagactg agttcccgga
      421 ggacagtctc ctcgcagagc tgcaaatcga aagttatgcc ggaatccctc tctggaattc
      481 gaacagacag tccatcggcc agattgctgt tctgggaaaa gagcctatca gcaaaccgga
      541 acttgtggaa tccattctta aaattgcagc agtgcgtgca tcccacgaaa tggaaagaat
      601 caaagcaaga gatgagctcg aacagagaat ccgtgaaaga acggaagaac tcaatcttgc
      661 gaaggaacag gcagactctg cgacaagagc caaaagcgaa tttcttgcca atatgagtca
      721 cgaaatcaga acccctctga atgccgttct gggattcaca gcacttcttg ccagaggccc
      781 actgacagaa gtgcagaaag aacatctgaa aaatctcagc tctgcagctg atattctgct
      841 gcagctgatc aatgacatta ttgatttttc aaagattgaa gccggaaaac tggaactgga
      901 aaaagcagaa ttcaatctgg gagaagtgtg caggaaaatc tcttccatat ttgcattcaa
      961 ggctcaggaa aaaggactcg aacttgtatt ttatctgaaa aacagtgtcc ctcttcatct
     1021 gataggagat tctcttcgtc tggaagaact gcttctgaat attctgggaa atgcagtcaa
     1081 gttcacggaa aagggaagca ttgttcttca gatcggactg gaagaagaaa cagaatcaga
     1141 tgcgctgatt tatttttcca tcaaggatac tggaatagga atgactgaag aacagagaaa
     1201 aaaactgttc actgcattca ctcaggcaga cagttcgact acaagaaaat tcggcggaac
     1261 cggtcttgga ctgacaatca gcaagaaact tgcaatgctg atgggagggg atatctgggt
     1321 acagagcaaa ctgggcaggg gctcaacatt ccgatttaca gtcaggatga caaaacagaa
     1381 aaatccggac atacagacac ttgaaattcc cgattttgtc agaggcactg atgttctgct
     1441 gattgaggaa aatccggatg ctgcacttgc catttcagaa tatctccagg cactttcatt
     1501 caacgcgaaa atttatcttt ctgttgatgc tgcggcagaa agtctgaaat tccctaaagg
     1561 aaaagagaag caggaattca gtatggtttt cgtggaaagc aggcttttct cttcaggaaa
     1621 aagaatcata tactctatgt tcaaagacag tccagtgaaa ccgaaggtaa ctgtgcttac
     1681 aggatat
//
LOCUS       DAOQGJ010000995         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52409_length_1683_cov_3.472359, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000995 DAOQGJ010000000
VERSION     DAOQGJ010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52409_length_1683_cov_3.472359"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            740..1135
                     /locus_tag="PK683_14380"
     CDS             740..1135
                     /locus_tag="PK683_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09684.1"
                     /translation="MNLIFQEQIRLTVTQVGIEKLGLLSDLISSFLTSGGRKLQKILE
                     TGDLYERSELLLVLLKEEIELNQIHKKIEKQIEEKLNRQQRDFLLREQLKAIKKELGL
                     EKEDKDSEAEIIEKNQKTCPDRRGIQSHQ"
     gene            1272..>1683
                     /locus_tag="PK683_14385"
     CDS             1272..>1683
                     /locus_tag="PK683_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09685.1"
                     /translation="MLNEQHYGLADVKQRILEFISTIIKRGRVSGSIVCLVGPPGVGK
                     TSIGKSIADALGRKFYRFSVGGMRDEAEIKGHRRTYIGAMPGKFIQSLKRTEVSNPVI
                     MLDEIDKIGTSYQGDPAAALLEALDPEQNHEFLDH"
BASE COUNT          583 a          310 c          374 g          416 t
ORIGIN      
        1 tattcataat cttggaattt ctgcgggtaa actgaattcc tggatactgg ggagtctcct
       61 ctgtggtaaa atatctgtta attttcataa aaagatggca cctctgcaat tatgtctcat
      121 gacagtatgc cggaaactga gttttttcac tattaaaaat ttaaaaaagt gaagggtttt
      181 tatgcgaata ataccgacaa gcaattgcga gattttgaaa aattttattg accgaaaaaa
      241 cagaaaaaca cctttgcatt ttccatctct tttcagagga aattccgttg aaacaggatt
      301 cctggaatgc acagacagga gtgttttacc gtaaaaaatc aatctatggc tgcagtttcc
      361 tgtgatccta aaaaaggtgg agattcgaac tgtaccgttt tttgaccgct tacgcagagg
      421 catattgagc tctgcattga aaaaaacaaa gggtatgctg ctgtctcact gattgaagaa
      481 aaggatgagg aaaatatctt cagtccaggc ttttcagaac aggatctctc atccgaatcc
      541 agaaaataat cagcaggacg gaagataaca tcagtttctt cgcacaggtt cttacgaggg
      601 tgcatcttga gaagcagacc gcatctgaag aagttcagta ctggcaggtc agttttgaat
      661 atgaggaaaa aaggaaatca cttctgagct gaaggcatat tcgcctgcta tcattaattc
      721 tgttaaagaa ttaattaaaa tgaatcttat attccaggaa cagatccggc tgacggtcac
      781 acaggtcgga atagagaaac tgggacttct ttcagatctg atctcctcct ttctcacatc
      841 cggcggaaga aaacttcaga agattctgga aacaggagat ctgtatgaaa ggagtgaact
      901 tcttcttgtt ctgctgaagg aagaaattga actgaaccag atccataaaa aaatagaaaa
      961 gcagattgag gaaaaactga acagacagca gagagatttt ctcctgcgtg aacagctgaa
     1021 ggccataaaa aaagaacttg gactggaaaa ggaagacaag gattcggaag ctgaaattat
     1081 agaaaaaaat cagaaaactt gtcctgacag aagaggcatc cagagtcatc aatgaagaac
     1141 tggaaaaact gaaaacacag gaaagcagtt cttcagaata caatgtaact aagacatatc
     1201 tcaactggct cactgatctg ccgtggggaa tcttttcaga agacaatttc gacattcaga
     1261 aagccaggga aattctgaat gaacagcact atggactcgc agacgtaaag cagagaatac
     1321 tggaattcat aagcacaatt ataaaacggg gccgtgtttc cggatccatt gtatgccttg
     1381 tcggtcctcc cggcgtagga aaaacatcga tcggaaaatc aattgcagat gctctgggaa
     1441 gaaaattcta cagattttca gtcggaggaa tgagagacga ggcggaaata aaaggacata
     1501 gaaggaccta cataggagca atgccgggca aattcatcca aagtctgaag aggacagagg
     1561 tttcaaaccc agtgattatg ctcgatgaaa tagacaagat aggcacaagt tatcagggag
     1621 atcctgctgc tgcacttctg gaagcactcg atcccgaaca gaatcatgaa ttcctggatc
     1681 att
//
LOCUS       DAOQGJ010000996         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52465_length_1682_cov_4.089736, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000996 DAOQGJ010000000
VERSION     DAOQGJ010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52465_length_1682_cov_4.089736"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PK683_14390"
     CDS             complement(<1..544)
                     /locus_tag="PK683_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH09686.1"
                     /translation="MIIKKALIVDDDTEIIECIASVLTELGSDEIIQISNSMKLQENY
                     GNDLDLLVIDLFMPELDGIEILRYLGEMKSRSFIILISGQSKQVLASAERIARAYGLN
                     VLGTLAKPFSINSLIELVEKAENFSSRTRNMRMQGLDFTEEDLREAVHDKKIIMYYQP
                     QIDLRKKIVSGFEALVRWQHP"
     gene            complement(541..>1682)
                     /locus_tag="PK683_14395"
     CDS             complement(541..>1682)
                     /locus_tag="PK683_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH09687.1"
                     /translation="KMEALGQIAGGVAHDFNNILAVIMGNVELLERKIQNLSDESMKK
                     NIQKTLNAVERGSHLTSKLLSFARKQNLNQETADINHILQDMKDMIERVIGKHIQIIL
                     DLYSEPLPIMIDKNEMENVILNLAINARDAMTDSGTLTIRTLKRDRISAPDSDYVFKP
                     GKEYAEITVSDTGRGIPAEIIQKIYEPFFTTKGAGKGTGLGLSMTYSFVKQFKGFISV
                     KSTVNRGTSFTLSFPLQSQEITEADALDSYSEEKPVSKNRGSVLIVDDEPSVLEIASS
                     ILAESGYIVMKAESAQEALSILSEKDADIVITDIIMPGEINGLELARIIRKEKPYIRI
                     ILTSGYPGDLRKKENSDLSEFTFIAKPYKALDLEKTVRKELDKKS"
BASE COUNT          443 a          387 c          291 g          561 t
ORIGIN      
        1 gaggatgctg ccaccttacc agcgcctcaa aaccggaaac aatttttttt ctaaggtcta
       61 tctggggctg ataatacatt ataatttttt tatcatgaac agcctcgcga agatcttcct
      121 ctgtaaaatc cagaccctgc attctcatat ttctggttct gctgctgaaa ttttcagctt
      181 tttccacgag ttcaataaga ctgttgattg aaaagggttt tgccagagtc ccaagcacat
      241 tcaggccgta tgccctggcg atcctctccg cacttgcaag cacctgcttg ctttgtccgc
      301 tgatcagaat aatgaaagat ctgcttttca tctctccgag atatctcaat atctctatcc
      361 cgtcaagttc gggcataaaa agatcaatga ccagcagatc cagatcgttt ccgtagtttt
      421 cctgaagttt catcgaattg gatatctgaa ttatttcatc agatcccagc tctgtcagca
      481 cacttgcaat acattcaata atttcagtat catcatccac gattaatgct tttttaatta
      541 tcatgatttt ttatccaatt cttttcttac cgtcttttca agatcaagcg ccttgtacgg
      601 ctttgctata aatgtaaatt cgcttagatc ggaattttcc ttttttctga gatccccggg
      661 atagcctgaa gtaagaatga tcctgatata cggtttttcc ttccgaatta ttctggccag
      721 ttcaagaccg ttgatttctc caggcataat gatatcagta attacaatat ctgcatcctt
      781 ttcagaaaga attgacagtg cttcctgggc actctctgcc ttcattacaa tatatccaga
      841 ttccgcaaga atcgaagatg caatttcaag cacggaaggt tcatcatcta cgataagcac
      901 actccccctg ttttttgaaa ccggtttttc ttcggaataa gaatctagcg catctgcttc
      961 cgtaatttcc tgactttgaa gcggaaacga cagagtaaac gatgttcccc tgtttacggt
     1021 gctcttcacg ctgataaatc ctttgaactg ttttacaaaa ctgtaagtca tggaaagccc
     1081 aagcccagtt ccttttcctg cccccttagt tgtaaaaaat ggttcatata tcttctgaat
     1141 tatttccgct ggaatgcctc tgcctgtatc agaaactgtt atctctgcat attcttttcc
     1201 aggtttgaaa acataatcag agtcaggagc tgaaatcctg tctcttttca aagtcctgat
     1261 tgtaagagtc cctgaatctg tcattgcatc tctggcattg atggcaaggt ttaaaatgac
     1321 attctccatc tcatttttat caatcatgat agggagcggt tccgaataga ggtccagaat
     1381 aatctgtata tgctttccaa tcactctttc aatcatatcc ttcatatcct gaagaatatg
     1441 attgatgtcg gcagtttcct ggttcaaatt ctgctttctg gcaaaggaaa gaagcttgga
     1501 agtcagatgt gaacccctct caacggcatt cagagttttc tggatatttt ttttcatgga
     1561 ctcgtcgctc agattctgaa tttttctttc aagcagttct acatttccca taattacggc
     1621 aagtatatta ttaaaatcat gagcaactcc gcctgcaatc tgaccaaggg cctccatttt
     1681 ct
//
LOCUS       DAOQGJ010000997         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52710_length_1678_cov_2.735059, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000997 DAOQGJ010000000
VERSION     DAOQGJ010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52710_length_1678_cov_2.735059"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PK683_14400"
     CDS             complement(<1..469)
                     /locus_tag="PK683_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNH09688.1"
                     /translation="MKAFIAPNHNPLLGWMADTLFHPLLKAMHNINEVIISESDKAAL
                     RTLKDHRLLFFTNHPSTAEPPLVYHMSNIIGARFKYMASRQVFDWGMGMVGKAISNIG
                     AFSVIAGINDKESLKTARAALAEKSGKLVLFPEGEPTSGENDNLMPFQEGIAAL"
     gene            694..>1678
                     /locus_tag="PK683_14405"
     CDS             694..>1678
                     /locus_tag="PK683_14405"
                     /inference="COORDINATES: protein motif:HMM:NF039306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HNH09689.1"
                     /translation="MPFEIDEKKRLGADDVKKKKEGWYVTGLAGPFSDPNSGSGIGGR
                     VLLFNNKTKDDPFFEYTPYRHRIFFNPSMTNKNAHYHWLDWDAPFIFDSQWRVRTSLI
                     YDRNPNNLFFGIGESSMNGLSNRFHNQRSLPMMDNQSFQDNQNNLNYARDPRSPNEIR
                     FNSDPFLNSLIQAQGTAGSNFKITDRMYNRYTLEVPQVQFSGERSFFKGLVRFVFGAR
                     LSKNTVKTYDGQLYKQVDPLFGEGPDMAQKFMLPSIQGKTKVTEDSEAKKIVGLNGGY
                     VNMLRIGLVYDTRDFEPDPGKGVFAEVTHERSDAAWGSNYNFNRTYGSFRSF"
BASE COUNT          476 a          348 c          392 g          462 t
ORIGIN      
        1 tgagagcagc aatcccttcc tgaaaaggca ttaaattatc attttcaccg cttgtcggct
       61 ccccttccgg gaaaagcacc aatttgcctg atttttccgc aagtgcggct ctggcagtct
      121 tcagagattc cttgtcgttg attcctgcaa tgacagagaa agcaccgata tttgagatcg
      181 cttttccaac cattcccatt ccccagtcaa atacctgtct ggaagccata tatttgaatc
      241 tggctccgat gatgttcgac atatggtaga ccaaaggcgg ttctgcagtg gaagggtggt
      301 ttgtgaagaa cagaagtctg tggtctttca gggtgcgcag agcagccttg tcggattctg
      361 aaatgatgac ttcattgata ttgtgcattg ccttcagaag agggtggaac agtgtgtcgg
      421 ccatccagcc tagaagaggg ttgtgattgg gtgcgataaa agctttcata agattggtat
      481 cttgcaaaat caacattctc agtcaagttg aaaaatttta cttgacttca aaaaaatgag
      541 gatgagagtt agctccattc cgtatctttg acagaaaatc catttcaatt caattggcgt
      601 atgagaagat tatataaaat tgcagcagcc cttgtgttca cacagggatt ggttttggcg
      661 caggattccc cctccccgtc gtcctcgaaa aaattgcctt tcgagatcga tgaaaagaaa
      721 aggcttggtg ccgatgatgt gaagaagaaa aaggaaggct ggtatgtgac cggtctggca
      781 ggtcctttca gtgatccaaa ttccggttcc gggatagggg gaagggttct tctgtttaac
      841 aacaagacaa aggatgatcc tttttttgaa tatactcctt accgtcacag gatctttttc
      901 aacccttcga tgaccaataa aaacgctcat taccattggc tggactggga tgcaccattt
      961 atttttgatt ctcaatggcg cgtcagaaca agtctgatct acgacagaaa cccgaacaac
     1021 ctcttctttg gaataggtga atcttccatg aatggacttt ccaacaggtt tcacaaccag
     1081 agaagtctgc ctatgatgga caaccagtcc tttcaggaca atcagaacaa tttaaactat
     1141 gccagggacc caagaagtcc aaatgaaatt cgcttcaaca gtgatccctt tctgaactct
     1201 ctcattcagg cgcaggggac tgcgggaagc aatttcaaaa tcactgacag aatgtacaac
     1261 aggtatactc tggaagttcc gcaggtgcag ttttccggtg aaagatcctt cttcaagggc
     1321 cttgtgagat tcgttttcgg cgcgagactt tcgaagaata ctgtaaagac atatgacgga
     1381 cagctgtaca agcaggttga tcctcttttc ggagaaggtc ctgatatggc tcagaagttc
     1441 atgcttcctt ccattcaggg gaaaacaaaa gtaactgaag attcggaagc aaaaaaaatc
     1501 gtcggactga acggcggtta tgtaaatatg cttagaatcg gtcttgtata tgatacaaga
     1561 gactttgagc cggatccggg gaaaggtgtt tttgctgaag tgacgcatga aagatcagat
     1621 gctgcatggg ggtccaatta taattttaac agaacatacg gttcattcag atcatttt
//
LOCUS       DAOQGJ010000998         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52776_length_1677_cov_2.541307, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000998 DAOQGJ010000000
VERSION     DAOQGJ010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52776_length_1677_cov_2.541307"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PK683_14410"
     CDS             complement(<1..1422)
                     /locus_tag="PK683_14410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNH09690.1"
                     /translation="MQYTEEQLRAINTLNKNLQIIACAGSGKTQVISKRIVNILKNPE
                     VSPGNIVAFTYTEKAAVELKNRILRIAEEEGLKRLGMAELFVGTIHAWCLKILQEYIL
                     EYQKFGVLDEIKLKLLLDRYYYSSTDRPSSGMKDLGLKIFTDTKIYIQLMTILRESEI
                     HHDQVPNQIKDALDKYERLLRRNCYFDFTMVLSELMNQLISNKELNSRLKEKIKYLVV
                     DEYQDVNPVQEKIIFHLYSLGANICVVGDDDQTIYQWRGSDLLNILSFEKRYNNVEII
                     KLEDNHRSTPAVVSVALKLIEHNQERKAKEMKAAGKLKYERGDVLFNSFEDPDSETDF
                     LISQIHSLRGISFQDKEHKRGLDYGDFCILVRAWSKAADIKDKLEKTNIPFIVSGVSQ
                     LFETAEVKAAKSIFEFLADKLDSGVVKDLWDAAGIKINDSNFASAVHFLEQSRPKKNQ
                     FFDTFCLQDIFQEFMEIAGITEDS"
     gene            1479..1677
                     /locus_tag="PK683_14415"
                     /pseudo
     CDS             1479..1677
                     /locus_tag="PK683_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
BASE COUNT          481 a          378 c          267 g          551 t
ORIGIN      
        1 ggaatcctca gttatcccgg caatctccat aaattcctga aaaatatcct gaaggcaaaa
       61 tgtatcaaaa aactgatttt ttttaggacg gctctgttcc agaaaatgaa ctgcactggc
      121 aaagttacta tcattgattt taatgccggc tgcatcccat aaatctttta cgacgccaga
      181 gtccagtttg tctgcaagaa actcaaagat agattttgcg gctttcactt ctgcggtttc
      241 gaacaactga ctcactccgc tgacgataaa tgggatattc gtcttttcta atttgtcctt
      301 aatgtctgca gctttgctcc aagcccgaac aaggatgcaa aaatctccat aatcaagtcc
      361 tcgtttatgc tctttatctt gaaaagaaat ccctcttaaa ctatggatct ggctgatcag
      421 aaaatcggtt tctgaatccg gatcttcaaa ggaattaaaa agaacatccc ctcgttcata
      481 tttcagtttt cctgcagcct tcatctcttt ggcttttcgt tcctgattat gttcaattaa
      541 tttcaaagca acagacacaa cagcaggagt gcttctgtga ttgtcctcca gtttaatgat
      601 ttccacatta ttatatcttt tttcaaaact gagaatattc agcagatcac tgcctctcca
      661 ctgatagatt gtctgatcat catctcccac aacacagata tttgctccca gagaatacaa
      721 atgaaatata atcttttcct gaaccggatt tacatcctga tactcatcca caaccagata
      781 ttttatcttt tctttcagtc tggaattcag ttccttattg ctgatcagct gattcataag
      841 ttcactcaga accattgtaa aatcaaaata acagtttctt cttaaaagcc tttcgtattt
      901 atccaaagca tctttgattt gattgggaac ctgatcgtga tgaatttccg attcccgcag
      961 aattgtcatc aactgaatat agatttttgt gtctgtaaaa atcttcagac ccaaatcttt
     1021 cattcctgag gacggcctgt ccgtactgga ataatagtat ctgtccaaaa gcagtttcag
     1081 tttgatttca tccaatactc caaacttctg gtattccaaa atatattcct gaagaatctt
     1141 caggcaccac gcatgaatgg ttccgacaaa aagctctgcc attcccagtc tttttaaacc
     1201 ttcctcttct gcaattcgta gaattctgtt tttcagttca acagcagcct tttctgtata
     1261 agtaaatgct acgatatttc ctggagatac ttcagggttt ttaagaatat ttacaattct
     1321 tttagaaatc acctgcgttt ttccggaacc cgcacaagca atgatctgca gatttttatt
     1381 cagtgtattg atggctcgaa gctgttcttc tgtatattgc ataattaata tttcaaattc
     1441 tcgttcttcc tttactttta caatgatttt ttaatatcaa tgcaacagtg gatctgcgct
     1501 acctctggat gagacagaaa cgtctgcagg gttcccattt tgcaaacgat gagaattcaa
     1561 aaggcttcaa cgacctggtc atcgccggca aagtggatcc ttgtctttcc agaacattca
     1621 agttcgaaga aactgctctc tctcaccagc tgatgagaga aaacaaacac cctcacg
//
LOCUS       DAOQGJ010000999         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52818_length_1676_cov_3.513263, whole genome shotgun sequence.
ACCESSION   DAOQGJ010000999 DAOQGJ010000000
VERSION     DAOQGJ010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52818_length_1676_cov_3.513263"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1071)
                     /locus_tag="PK683_14420"
     CDS             complement(253..1071)
                     /locus_tag="PK683_14420"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HNH09691.1"
                     /translation="MPYFEEIRLKTWKDARKFFDTILNNSQKWVFRGHYKSNWKLQPS
                     LERIVSRKLPGMETESKIISEFRRRAHQYLQPSEIPSSLLEWISLMQHHGAPTRLLDW
                     TKSPYVASFIAFENAYKEKGEIAIWALDANWLFNASLARVQKANLNLNFRLIQNDSEF
                     IRFINSEARVVLPVEPKLMNERLTIQQGIFLIASGKKLSFEKIIMEYREADSVDHIKK
                     IVLPKSEGISCLTELNRMNINAATLFPGLDGFARSLRTIPDITYADYCGENEIF"
     gene            complement(1074..>1676)
                     /locus_tag="PK683_14425"
     CDS             complement(1074..>1676)
                     /locus_tag="PK683_14425"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09692.1"
                     /translation="DSAAEELKATGRASPIHVDSASVMFTDFSGFTAISSAMNPADLI
                     EELDICFSAFDLITEKYGLERIKTIGDSYLCVGGLFESSPHIENSCSAALEILQFMKE
                     RKEKKESEEGRAYWNIRIGLHTGPLAAGVIGKKKFAFDIWGDTVNTASRMESAGEPGM
                     INVSEQIYSAAKDFFSFSYRGEMEVKGKGRMKMYKLESDG"
BASE COUNT          421 a          381 c          351 g          523 t
ORIGIN      
        1 agaaagaatt tactttctat gcttcttttc tcggtaactc agcttacgcc taatgctgtc
       61 aagataagga tgattcaccg cagatgcaca cggattaacg cagataatga atcaaaatat
      121 ccgtgttgat ctgtgcatat ccgtggtaat ctttttaatt ttcttcttaa gttgacagcc
      181 ttaagtttac gccttcggct tttttagagg agagcctgac ggggtttcgc aaacggggag
      241 actgcccgat tttcagaaaa tttcgttttc cccgcaatag tctgcatatg taatatcagg
      301 aatagtacga agcgatctgg caaatccgtc caggccggga aaaagagttg cggcattgat
      361 gttcattctg ttaagttccg tcagacagga aattccttcc gacttgggaa gaacgatttt
      421 tttgatatga tccacagaat ctgcttcgcg atattccata atgatttttt caaaggaaag
      481 ttttttcccg cttgcgatca ggaatatccc ctgctggatt gtgagtcttt cattcatcag
      541 tttgggttcc acaggaagga caaccctcgc ttccgaattg atgaaccgga tgaattctga
      601 atcattctgg atcagacgga agttcaggtt cagatttgct ttctgcaccc tggcaagaga
      661 tgcattgaac agccagtttg catccagtgc ccagatggca atctctccct tttccttgta
      721 tgcattttcg aatgcaatga atgaggctac gtaaggagat tttgtccagt cgagcagtct
      781 tgtaggtgca ccgtgatgct gcatcagtga aatccattcc agaaggctgg acggaatttc
      841 tgaaggctga agatactgat gtgcccgtct tcgaaattca ctgatgatct ttgattcagt
      901 ttccatgcct ggaagttttc tgctgacaat tctttccagt gacggctgca gtttccagtt
      961 ggatttgtaa tgtcctcgaa aaacccattt ctgactgttg ttcaatatgg tatcaaaaaa
     1021 tttgcgggca tccttccaag ttttcagtct gatttcttca aaatacggca taattatccg
     1081 tctgactcca gtttatacat tttcattcgg ccctttcctt ttacttccat ctctccccga
     1141 tacgaaaagg aaaagaaatc cttcgctgct gaatagatct gctcagatac attgatcatt
     1201 ccgggttctc ctgcagattc cattctgctt gcagtgttga cagtgtctcc ccagatatca
     1261 aatgcaaatt tcttttttcc gatgactcct gccgcgagag gaccggtatg cagaccgatc
     1321 cggatattcc agtaagctct gccttcttct gattcttttt tctctttcct ttctttcatg
     1381 aactgcagaa tttccagtgc ggcagaacag gaattctcta tatgcgggga actctcaaat
     1441 aaacccccga cgcaaagata gctgtcgcct attgttttta ttctttccag tccgtacttt
     1501 tccgtaatca gatcaaatgc agagaaacag atgtcgagtt cctcgatcag atccgcagga
     1561 ttcatcgcag atgaaattgc agtgaatccg gaaaaatcag tgaacatcac tgaggcagaa
     1621 tctacatgaa tgggggaagc tctgcctgtc gccttcagtt cttctgcagc actgtc
//
LOCUS       DAOQGJ010001000         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52843_length_1676_cov_2.919803, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001000 DAOQGJ010000000
VERSION     DAOQGJ010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52843_length_1676_cov_2.919803"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            538..1641
                     /gene="mtnA"
                     /locus_tag="PK683_14430"
     CDS             538..1641
                     /gene="mtnA"
                     /locus_tag="PK683_14430"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNH09693.1"
                     /translation="MQNTSQKFLSSIVWRYDHLLLLDQRKIPKEKAYLKMTTLEEVVE
                     CIRLMVVRGAPAIALSGMYGLTLFVRRSREKPSYSEFQAKCSFLLDSRPTAVNLKLAL
                     DRLNKEINESVYSSLSLSELTDRIESLSNLMFKEDYDTNRKISENGAALFGEPREINI
                     LTHCNTGAVATAGIGTALGVVRALRDRGFKVTVYADETRPYLQGSRLTSWEMMEEGID
                     CFIIADSMAAWLMKDRRIDAVIVGADRIASNGDTANKIGTYGLSILAKSHNVPFYVCA
                     SETSFDFSLNDGSGIVIEMRNEDELTQYSFLKDSEGKNLVEKGVFSPKNARALNPGFD
                     VTPSENIAGIITEKGIISPVNRENTSKVILSGK"
BASE COUNT          501 a          293 c          375 g          507 t
ORIGIN      
        1 tgctttcgga aaattattgc atctagatag catattgtat ttgcgtaagg tgagttattt
       61 actagtaatt taaaatctgg tcatgctatg tataatttaa aaataatgat attaatgtga
      121 ttcatgtctt ttctcttctg acgttccata taactttttt cagcgggaag tttttatttt
      181 ttcggactca actgaactgg gatatgaaaa aatctgggaa tagcagtatt gattcattcc
      241 ttgcataata agctgtaatg ccggacatgt tttaaggatt gtcaggactg aaaatgccgc
      301 attcggaaaa agttttgcga atgaaaaatc attctccgct ctgaaatttt cagagagatc
      361 aaatgtatta cagtttagat ttcctccatc tgcaggactg aaggtaagac tgaatttatt
      421 gtcagtgtta tgacagatat aatataatga actgaatctc cctttgcgca atgttcagga
      481 aaagcagtaa aactttttac ttgtttttca gactgagttt tgaattatga acagtatatg
      541 cagaatactt ctcagaaatt tttatcttcc atagtctggc gttatgatca tctcctgctt
      601 cttgatcagc ggaaaattcc gaaggaaaaa gcttatctta agatgactac gctcgaagaa
      661 gttgtggaat gcattcggct gatggttgta cggggagctc ctgccattgc attgtcggga
      721 atgtacggac tcacactgtt tgtaaggcgg agcagggaaa agccgtccta ttctgaattt
      781 caggcaaagt gttcctttct tctggattca agacctactg cagtcaatct gaaacttgcg
      841 ctggacagac tgaataaaga gatcaatgaa tcggtctaca gttcactgtc gctctcggaa
      901 cttacggaca ggattgagtc attgtcgaat cttatgttca aggaagatta tgatacaaac
      961 aggaagatct ctgaaaacgg agctgctctt tttggagaac cccgcgaaat aaatattctg
     1021 actcactgca acactggtgc tgttgcaact gcgggcatag gtactgccct tggagttgtc
     1081 cgggctctta gggacagagg cttcaaggtc actgtttatg cggatgaaac gaggccttat
     1141 cttcagggat caagacttac ttcatgggag atgatggaag aaggcataga ctgttttatt
     1201 attgcagact caatggcagc atggctgatg aaggacaggc gtatcgatgc agtcatcgta
     1261 ggagcagaca gaattgcatc aaacggcgat actgcaaaca agattggaac atacggtctt
     1321 tccatacttg caaagtctca taatgttcct ttctatgtat gtgcaagcga gactagtttt
     1381 gatttcagtc tgaatgacgg ttccggcatt gtgattgaaa tgagaaatga ggatgaactg
     1441 actcagtatt catttctaaa agacagcgag ggaaaaaacc tggtagaaaa gggagttttt
     1501 tcgccgaaaa atgcaagggc gctcaatccg ggatttgatg tgactccttc agaaaatatt
     1561 gcaggcatca ttacagaaaa gggaattata tctcctgtaa acagagaaaa tacctccaaa
     1621 gtgattcttt cagggaaata gactgcgcga tatatcgtca gtatattata ttctat
//
LOCUS       DAOQGJ010001001         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52856_length_1676_cov_2.385564, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001001 DAOQGJ010000000
VERSION     DAOQGJ010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52856_length_1676_cov_2.385564"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(698..875)
                     /locus_tag="PK683_14435"
                     /pseudo
     CDS             complement(698..875)
                     /locus_tag="PK683_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077143899.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1051..>1676)
                     /locus_tag="PK683_14440"
     CDS             complement(1051..>1676)
                     /locus_tag="PK683_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH09694.1"
                     /translation="LFVVSIVAVFTGMIMGLNAGLGLKDFGAEGQIGYLLTITLTREM
                     SPFMTALILSASVGSAIAAEIGTMKVSEEIDALEVMSIDPVRYLVMPRILGFSLMVPV
                     LCVYSTMLGVLGGAVVGHFQLGVDFKIYFRDVFERVSSPQGLKDLYVGLFKGFVFGII
                     VSSIACSHGLRTSNGAIGVGRATRESVVTSFLVVIFFGYVLTALFYR"
BASE COUNT          510 a          379 c          311 g          476 t
ORIGIN      
        1 gtattttctc cgatccagcg ttcagtgact gaaatgcctg acctaaatta taattaattt
       61 cacttaataa atcattgttt ttatcttcag gctttctttc agaaaaattc agtccgattt
      121 catcggatac gtcattcaga aaataactcc cctgttttga gggaagttct tctgaattcc
      181 gtttcagatg ctcagactcg gattcagctt ttttgcggag gtccataaaa ttttccggga
      241 agggaacaat attgagaact ttgttcctgt gaatcagatc gatgatttct ctcagagcca
      301 gctcaattcc gttcagatcc tgtgttcgga agaatttcgg catttccgat gacatataga
      361 aggaatagaa atgattattt ataatgattt tatatactcc atttccggaa ctgagagcag
      421 aatctgtatc tgtaattctg aggagcacat ctgtcagtgt attttccaga cgtctgattt
      481 cattctgaat ttccggatgg gaaatttttt caggaactga tgaaccgata taataactgt
      541 acatactgtt ccagaaattt actttatgaa tcttattctt ttttgtaatc ccgtagctta
      601 aaatcccgag cagcctgttt cttctgatga agaatccttt ttctgatgca ggtatccata
      661 tatcctccgg tgtctcttcc agaagaaatt caggaagatc ggacagctga atcagttttt
      721 caagattttt caagttctct cctttcgttt ctccagtctc tcagaacata ataacatatc
      781 cctagcgatt cagatacttc ttttatcgta aaactgccga tcaccgtccg tttcactgcc
      841 tgttccttga actctaggag ttcgccatcc cccattttca gtgacgacaa aaatggtcgg
      901 taaggagact gcagactgat aaaacgggac agatatgcac ttgaatacaa tggaaaaatt
      961 ttccagtgaa cttaagacaa agaattcatc cggaatatct ctccgagttc tgcagtctgc
     1021 gatgagcgca ggagaatatg atgagtccta tcacctgtag aaaagtgcag tcagcacata
     1081 accgaagaaa atcactacaa gaaatgaagt taccactgat tcccgagttg cacgtcccac
     1141 tccaattgca ccgttggatg ttctaagacc gtgagaacag gcgatgctcg aaacaatgat
     1201 cccgaataca aatcctttga aaagaccgac ataaagatcc ttcagtccct gaggtgaaga
     1261 aactctttca aatacatctc tgaaatagat cttaaagtcc acacccagct gaaaatgtcc
     1321 tacgactgca ccccccagca ctccaagcat tgtagaatag acacagagca caggaaccat
     1381 cagagaaaaa ccgagtattc tgggcattac aagatatcgg acaggatcaa tcgacatcac
     1441 ttcgagagca tcaatttcct cggaaacttt cattgttcca atttcagcag caattgcaga
     1501 accgacagat gcagacagaa tgagagcagt catgaacgga gacatctctc tagtcagcgt
     1561 aatcgtcaga agataaccaa tctgtccttc cgcaccgaag tctttcagac cgagtccggc
     1621 attcagaccc ataatcattc ctgtaaatac agccacgatg gaaacaacaa acagac
//
LOCUS       DAOQGJ010001002         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52863_length_1676_cov_1.844540, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001002 DAOQGJ010000000
VERSION     DAOQGJ010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52863_length_1676_cov_1.844540"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PK683_14445"
     CDS             <1..294
                     /locus_tag="PK683_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09695.1"
                     /translation="GENIYDRDNMFKDYLSSQDMKKSFYCGKHSITVHDISLYAVFSG
                     WKEDLEKFLREREIEWTVTDMQKLEEDFPSSISAKILKKINGAESANLPFPTE"
     gene            701..>1676
                     /locus_tag="PK683_14450"
     CDS             701..>1676
                     /locus_tag="PK683_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNH09696.1"
                     /translation="MNKIIAKTYRYLKANSGKDISWNLLMEDLAEKKKDKKHGKKHSG
                     DHKEDLMIFLNFLESELLIEKHKKHLHVSKNFKITGKIFLSKWGDGFVKLRSQKEVFV
                     PREFTEGAIPGDTVEIIPLGFGKRGRLEGEVREVIKRGRTFYRLKIVDADEHYFYGII
                     LDQNGEEKEGFLRKKSLLADVVRSIKKDDVLIVKFREDSFQSGNLYETSFLRFEEGDS
                     RDRDYNRILLKYDFHQNYPEEIILNFPENPGPENTEDWGERADLRNLWTATIDGATSK
                     DFDDAVSIETEGDIVRFYVHIADVGHYVRLHTPLDEEALKRATSVYLTD"
BASE COUNT          531 a          312 c          402 g          431 t
ORIGIN      
        1 ggagagaata tatatgaccg tgacaatatg ttcaaagatt atctgagcag tcaggatatg
       61 aaaaaatctt tctactgcgg gaaacattcc atcacagttc acgacatcag tctttatgcc
      121 gttttttcgg gctggaagga agatctggaa aaatttctcc gggaaaggga gattgaatgg
      181 acggtgacgg atatgcagaa actggaagag gattttccat catcaatttc cgccaagatt
      241 ctgaaaaaga tcaacggtgc tgaatcggca aatctgcctt ttccgactga ataaaggact
      301 ctgtcctccc ggaactccgg gaatacatta taaagtattg cgatactcgg gaattacgga
      361 tactttgctt catattcagt cagtcttcca ccggatgaat atctctgtat tttaatgaaa
      421 tattcatgca ttcagcgcag cacagactga gtgtattttc attttcgctg aattgggctg
      481 cagaaaaagt gcagacagcg atatctgtgc attcagattc agagaaggca gttttctttc
      541 cctgcggttc ctgcagattc tcaaaagacg aaggacagag atgccggcgt ttctgctccg
      601 tccatccgga aaatcggagc atatggaaac atactgcgga tctgcaattt tcacttgcac
      661 ggaaagctga ttcagaatta tctgaagaga gaaaggattt atgaataaaa ttattgcaaa
      721 aacttacaga tatcttaagg caaactcggg aaaggacatt tcctggaatc ttcttatgga
      781 agatcttgct gaaaagaaaa aagacaaaaa acacggaaag aaacattcag gagaccataa
      841 ggaggatctg atgatctttc tcaactttct cgaatcggag cttctgattg aaaagcacaa
      901 gaaacatctc catgtgtcaa aaaatttcaa aatcacggga aaaatttttc tttcaaaatg
      961 gggtgacggt tttgtgaaac tgagatctca gaaggaggtc ttcgttccca gagaatttac
     1021 cgagggggcc attcccggag acacagtgga aatcattccg cttgggttcg gaaaaagagg
     1081 caggctggaa ggtgaggtcc gcgaagtcat taaaagagga agaacatttt acagactgaa
     1141 gatagtcgat gcagatgaac attattttta cggaatcata cttgatcaga acggagagga
     1201 aaaagaggga ttcttaagaa agaaaagcct gcttgccgac gtcgtgcgga gcatcaaaaa
     1261 ggatgacgtt ctgattgtca aattcagaga ggacagtttt cagtcaggca atctttacga
     1321 gacaagcttt cttcggtttg aggaaggaga ttcgagggac agagactata acaggattct
     1381 tctcaagtat gattttcatc agaattatcc tgaagaaatc attctgaatt ttcctgaaaa
     1441 tccgggacct gaaaatacgg aggactgggg cgaaagagcg gatcttcgaa atctctggac
     1501 tgccacaatt gacggagcga catccaagga ttttgacgat gcggtaagca ttgaaactga
     1561 gggagacatt gtccgttttt atgtgcacat tgcagatgta ggtcattacg tccgtctgca
     1621 cacacctctg gatgaggagg cactgaaaag agcgacgtcg gtctatctga ctgatc
//
LOCUS       DAOQGJ010001003         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52885_length_1675_cov_3.628395, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001003 DAOQGJ010000000
VERSION     DAOQGJ010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52885_length_1675_cov_3.628395"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1108)
                     /locus_tag="PK683_14455"
     CDS             complement(125..1108)
                     /locus_tag="PK683_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09697.1"
                     /translation="MNKTEADRRFNIFFIIKNSNKILLSFFLLLTLSCSSFMQKKNRS
                     EKTFPEICFGIREKNTKLSIRPEKIPSDDLETLRKHFSNNAISIANDIGVTAQLLEYL
                     SLGGKNKSHNRKLFIMKEVQERVTLSQMELSSMTAIADCERGRAMQLASHLDTLTNDE
                     TNTLAVRSILSTVVGTVLSAAIELTGGTAQLYKAADLFFAFLAGHFAYQSLNLDIKGY
                     LGHRNNILRELKDKPEKPELFYASVWNFLNNENYPNEISERGEILRLWEQNKGTEEDE
                     KLIFSEGGDYTSGQLYKLVSKYDSLKIRMAYMQLELRQLLEELEKKEKEIL"
     gene            complement(1244..>1675)
                     /locus_tag="PK683_14460"
     CDS             complement(1244..>1675)
                     /locus_tag="PK683_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09698.1"
                     /translation="LLAAAGLSPSELKPVILDFLSNPETSNTAFRTVSRHRILALNSD
                     IEKIFLNTENMQIQKNCLKLLLSSSKWDSLLYILRYLKTEGETGAICRSALDRWLLNF
                     NKSFILPDSAQKERILSEIPVTAAGRNSEIFRSVLSILKHF"
BASE COUNT          410 a          328 c          366 g          571 t
ORIGIN      
        1 atgagcggaa tttcagggat agggtcttat actgcagaca gaaacgggtt tgtcatggag
       61 ctggaatgca gttttgacct ctcggctgaa tgatgccggt ctgctgatcg gcggtttaga
      121 tgaatcagag aatctctttt tcctttttct ccagttcttc gagaagctgt ctgagttcca
      181 gctgcatata cgccattctg attttcagtg agtcatactt tgatacgagc ttgtaaagct
      241 gtcctgaagt ataatctcct ccttcagaaa agatcagttt ttcgtcttct tctgttccct
      301 tgttctgttc ccagagtctg agtatttctc ctctttccga tatctcattc ggatagtttt
      361 cgttgttcag aaaattccag acagatgcgt aaaaaagttc cggcttttcc ggtttgtctt
      421 tcagttcacg gagaatattg ttcctgtggc cgaggtatcc tttaatgtca aggttcagtg
      481 actggtatgc aaaatgacct gcgagaaacg caaaaaacag atcggctgcc ttgtagagct
      541 gtgccgtgcc tcctgtaagt tcgattgctg cggagagaac cgttcctaca actgtggaga
      601 gaatggaacg gactgcaagt gtattggttt catcgttcgt gagtgtgtcc agatgagatg
      661 caagctgcat tgctcttccc ctttcacagt ctgcaattgc agtcatactg ctgagttcca
      721 tctgtgaaag cgtcactctt tcctgcactt ccttcataat gaatagtttc ctgttatggc
      781 ttttgttttt tccgcccagt gaaaggtatt caagcagctg tgcagtgact ccgatatcat
      841 ttgcaatgct tattgcattg ttcgaaaaat gtttcctcag tgtttccaga tcatcacttg
      901 gaattttctc aggtcttatg ctcagttttg tgtttttttc tcggatgccg aaacagattt
      961 cagggaatgt cttttctgat ctgttttttt tctgcataaa actggagcag gagagtgtca
     1021 gaagaagaaa aaagctcagc agaattttat ttgaattttt tataatgaaa aatatgttaa
     1081 atcttctgtc tgcttctgtt ttattcattc cgtttgttcc tgaccgagta ttgtatgttt
     1141 ttttatggat ttataaatta ttatataatt ataaatccgg acaggagaaa aatcccctgc
     1201 tttttggaca gttttttccg tgaatctgtc caatcaagaa tttttagaaa tgcttcagga
     1261 tggaaagaac agatctgaag atttctgaat tcctgcctgc tgcagtcact gggatttcag
     1321 aaagaatcct ttccttctgt gcactgtctg gcagtataaa ggatttattg aaattcagta
     1381 gccatctgtc cagtgcagat ctgcagattg cgccggtctc accttctgtt ttcaaatatc
     1441 tgagaatgta cagaagcgag tcccatttgg aagaactcag aagaagcttc agacagtttt
     1501 tttgaatctg catattctca gtgttcagga atattttttc tatgtccgaa ttgagtgcga
     1561 gaatcctgtg tctgctgact gtcctgaacg cagtatttga agtttccgga tttgatagaa
     1621 aatccaggat gacaggtttc agttcagacg gggaaagtcc tgccgctgca agaag
//
LOCUS       DAOQGJ010001004         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52954_length_1674_cov_3.649166, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001004 DAOQGJ010000000
VERSION     DAOQGJ010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52954_length_1674_cov_3.649166"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PK683_14465"
     CDS             complement(<1..1316)
                     /locus_tag="PK683_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09699.1"
                     /translation="MVRTFFILILVSVLNCATTHSEKMKGVEEALYAGDYEAALPKMR
                     SLVSGASSRDKLLYLMEAGVVLHTKGDYKASNKAFQDAEALADTAKTSVTGETLAFLL
                     SDDKNSFRGESFENVMIKMYIALNYLCLNELENAKRYFKKVSFEQKEMKFSEAKYRQN
                     LLARYLDALASEHLGQFNDARVEYKNILEIEPGFKEVLADRYVLAMKEKDGADQSKFG
                     EGKNYIDSYDKDMKKISYDSRMGELIVINQAGKAAVKESRGKLMDDPNIREPLSAAIE
                     GSLRSNSTEGIDLGIVLATLSSAEHPIPEYRDRDAQTGSEVKILINGKEAGKTRKLAD
                     YSGMAKSSFNDNLNSIITKNIASIATKAVTVAIAAQAAKKAAEGSGKDRNELLGSVVG
                     WVFSAIGGYGMAQTLAPDLRCWRTSPSNFQAKRIFLEPGDYTISFEG"
     gene            complement(1310..>1674)
                     /locus_tag="PK683_14470"
     CDS             complement(1310..>1674)
                     /locus_tag="PK683_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNH09700.1"
                     /translation="LATRNDTSEQIPTEIFDSALVKTLMKNKIYTLKVDARQDQLKQI
                     KWDQQMGIGQFNLGNLKSPNYFVKSRIDENMFRSDGQKVVEQSMNVEFIHLESLAVMV
                     SEKETFSKKAKDNQGLGW"
BASE COUNT          401 a          420 c          317 g          536 t
ORIGIN      
        1 ccctcaaaac tgattgtata atctcccggt tccagaaaaa ttctcttcgc ctgaaaattg
       61 gaaggactgg tcctccagca tctcagatct ggagccaagg tctgagccat tccgtatccg
      121 ccgattgcac tgaataccca tccgactaca cttcccaaaa gttcatttct atcttttccg
      181 cttccctcag ccgctttttt tgcagcctgt gccgcaatgg ccacagtgac tgcctttgtc
      241 gcaatggacg caatattttt tgtgatgatt gaattcagat tatcattaaa actgcttttg
      301 gccattccgc tgtagtcagc cagttttctt gttttgccgg cttcttttcc gttgatcagt
      361 attttgacct cgcttcctgt ctgagcatcc ctgtccctat attcgggaat cggatgttct
      421 gcagaagaaa gagtggcaag cacaattccc agatcaatcc cttctgtaga attggaacgg
      481 agagaacctt ctatcgcagc agaaagaggt tcgcggatat tcggatcatc catcagtttt
      541 cctcttgatt cctttactgc agcctttcca gcctgattga ttacaatgag ttcacccatc
      601 ctggaatcgt aggaaatttt cttcatatcc ttatcgtagg aatcaatata attttttcct
      661 tcaccgaatt tggactgatc tgccccgtct ttctctttca ttgccagaac atacctgtct
      721 gccagaactt ccttgaaccc cggttcaatt tcgagaatat tcttgtattc cactctggca
      781 tcattgaact gtccgagatg ctcagaggca agcgcgtcca aatatctggc aagcagattc
      841 tgtctgtatt ttgcttcaga aaatttcatt tctttctgct caaaggaaac cttcttgaaa
      901 taccgctttg cattttcgag ttcattgaga cagagataat tcagagcaat gtacattttg
      961 atcatcacat tctcaaagct ttctccccga aagctgtttt tgtcatccga aagaagaaat
     1021 gccagagttt caccggttac acttgttttt gctgtatcag caagagcttc cgcatcctgg
     1081 aatgccttgt tgcttgcctt gtagtcaccc tttgtatgga gcacaactcc tgcttccatc
     1141 aggtacaata acttgtccct ggaactggca ccggaaacaa gacttctcat tttgggcaga
     1201 gcagcctcat aatcaccggc gtacagggct tcctccacgc ctttcatttt ttcagaatgt
     1261 gtagtcgcac agttgagtac tgaaacaaga atcagaatga aaaaagttct taccaaccga
     1321 gtccctgatt gtctttggct tttttggaaa atgtttcctt ttcagaaacc atcactgcga
     1381 gagattccag atgaatgaac tccacattca tactctgctc caccactttc tgcccgtcgc
     1441 ttctgaacat attttcatca atcctggatt ttacgaaata gttgggactt ttcagatttc
     1501 ccaggttgaa ctgccctatt cccatctgct ggtcccattt gatctgtttg agctgatcct
     1561 gtctggcatc caccttgaga gtgtagattt tattcttcat cagggtcttt acaagagcac
     1621 tgtcgaatat ttcggtggga atctgctccg aagtgtcatt cctagtggca agaa
//
LOCUS       DAOQGJ010001005         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_52984_length_1674_cov_2.382952, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001005 DAOQGJ010000000
VERSION     DAOQGJ010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52984_length_1674_cov_2.382952"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1037
                     /locus_tag="PK683_14475"
     CDS             30..1037
                     /locus_tag="PK683_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09701.1"
                     /translation="MQLKEYFKLDWKRPADTEETVSNRQRRESLEKNLDGCFSRLEAA
                     EICSSNDKIPEALILLRSLKIQALNAVLSFFHQTEINDEKDYTQALSKIPDSELFSIF
                     EKIGAVSLYEENSETEEKAEEILSDSLYRIRKRIFAHYEQELKDPIRNFRKRVLFQSV
                     LSSVIIIVLAVSALREIKRKSPLKEDSAQISLSDKREGTPVVSDIPGRKAAFRPGSWN
                     TLKFSLEKPETVGSLRLDPLKQKDARIQIREISFFSKDGKNLFTKNLTVDASKIQSES
                     GNIILVGNISLGRLLQGQPIELVTTGENPSLLFKLEEIKNVSEIRVQMRAVRKSAQFK
                     N"
     gene            1039..>1674
                     /locus_tag="PK683_14480"
     CDS             1039..>1674
                     /locus_tag="PK683_14480"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNH09702.1"
                     /translation="MIKNFIILIRPHQWIKNFILFAGIIFGKKLNDPDAVMKVTSAFF
                     LFSFVASCQYVFNDYMDRAEDAMHPEKKNRPLASGAIDPGVALTLMIIMLTVSLILSF
                     LLSQIFFGLLIFYFLFNLTYSSYLKHLVILDVMSISIGFVIRAVAGAAVINVQFSSWL
                     LLCTFMLALFWGFSKRRGEIILMEEGAKTHRKILDEYSVNFLDMMLGITATM"
BASE COUNT          517 a          335 c          339 g          483 t
ORIGIN      
        1 gttcaatgaa aacgcttcag gagcaatgaa tgcaattaaa agaatacttt aaactggatt
       61 ggaaaaggcc tgcagacacg gaagagacag tatccaacag acagagacgg gaatctctcg
      121 aaaaaaatct ggatggatgt ttttccaggc tggaagcggc tgaaatctgc agttcaaatg
      181 ataaaattcc tgaagcactg attctcttaa gatccctgaa aattcaggct ttaaatgcag
      241 ttctctcttt ctttcatcag acagaaataa atgatgaaaa agactatact caggctctat
      301 caaaaattcc ggattctgaa ctgttttcca tttttgaaaa aatcggtgca gtcagtcttt
      361 atgaagagaa ctctgaaact gaagaaaagg cggaagaaat attaagtgat tctctttaca
      421 gaatcagaaa aagaatattt gctcattacg aacaggaatt aaaagatcca attcgcaact
      481 ttcgaaaaag agtattgttt cagtccgttc tgtcttcagt catcatcatt gttctggctg
      541 tgtctgctct cagagaaatc aaacggaaat ctcctctcaa ggaagactca gctcagattt
      601 ctttatctga caaaagagaa ggaaccccgg tcgtatccga cattcccgga aggaaggccg
      661 ctttcagacc tggcagctgg aacacattga aattcagtct ggaaaagcct gaaactgtcg
      721 gcagtctgcg tcttgatcct cttaagcaga aggacgccag aattcagata cgtgaaattt
      781 ccttcttttc gaaggatgga aagaatctgt tcaccaaaaa tctcactgtt gatgcatcca
      841 aaattcagtc tgaatcagga aacattattc ttgtcggaaa tatttcgctc ggcagactgc
      901 ttcagggaca gcctattgaa cttgtaacta ccggtgaaaa tccttctctc ctcttcaagc
      961 tcgaagagat taagaacgtt tctgaaatca gagtacagat gagagcagta aggaaatctg
     1021 cacaattcaa aaactgatat gataaaaaat tttataatac tcataagacc gcatcagtgg
     1081 atcaaaaact ttatcctttt tgcaggaatc attttcggca agaaactcaa tgatcccgat
     1141 gcagtaatga aggttacttc agcttttttt ctattttcct tcgtagccag ctgccagtat
     1201 gtcttcaacg attatatgga cagagcagaa gatgcaatgc atcccgaaaa aaagaacaga
     1261 cctcttgctt ccggagcgat tgatccggga gttgcactga cactgatgat tattatgctg
     1321 acagtctctc tgattctttc ctttcttctt tctcagatat ttttcggtct cctgatattc
     1381 tatttccttt tcaatctgac atacagctct tacctgaagc atctggtaat cctggatgta
     1441 atgtccatca gcataggatt tgtgatacgg gctgttgccg gtgcagctgt aatcaatgta
     1501 cagttttcat cctggcttct gctctgcacg tttatgctgg cacttttctg gggtttcagc
     1561 aaaagaagag gggaaatcat tctgatggaa gaaggggcaa agactcacag aaaaattttg
     1621 gatgagtatt ccgtaaactt tcttgatatg atgcttggaa ttactgcgac gatg
//
LOCUS       DAOQGJ010001006         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53026_length_1673_cov_2.949938, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001006 DAOQGJ010000000
VERSION     DAOQGJ010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53026_length_1673_cov_2.949938"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PK683_14485"
     CDS             <1..605
                     /locus_tag="PK683_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09703.1"
                     /translation="PALIGAFSNPDLEVRKILYREMLFTEMKNKGEDPRKKMESDPES
                     APVLELFRKKLFASEEKDPEAFVLREELIRQYNYDLVTAVRKKNDLAVAYMSPETLDS
                     EISKNEDKIMKKWIKRSLKIFIKALGNERCSVETKLKLIEIIHLSKYGGPAKLIPKAP
                     SLAKRADELFSIAEAEKDTKLIKKITDLGALYYNLALENK"
     gene            605..>1673
                     /locus_tag="PK683_14490"
     CDS             605..>1673
                     /locus_tag="PK683_14490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH09704.1"
                     /translation="MLDKLGLGDLRFHREGNPVLSFFLMTVLSFFLFADQNLIAPNLK
                     NIGRSLGLHTEQDINWYLGGLIPVSFFVLGGIVSLSMGVLSEKYPRKILLVSSILLGE
                     IPCFLTGFAENYTQFFILRTLCGFGLGGVFPILFSMVGDYFSEKSRVTASAYVSLAMG
                     LGVGIGQMAGGIFGESDPLNGWRQSFIWLSAPSFPIAGAYFLFCGEPKRGVFSSGNNE
                     HKLNFSDIKNIFKNSTNRALLLQGIPGCVPWGVFFVYLVDFYETVYGLGKTASSLYVT
                     LAGIGVMIGIILGGIIGQKLYNRSRRLQAVFAMSCILLGIVPAYFLIHGKWMSGTVFF
                     PVLNLLTGIVLTLPLSNLRSIL"
BASE COUNT          487 a          356 c          357 g          473 t
ORIGIN      
        1 tccctgctct gatcggcgca ttctccaatc cagatctgga agtaagaaaa atcctgtacc
       61 gggaaatgct ttttactgaa atgaaaaaca aaggagaaga tcccagaaaa aaaatggaat
      121 ccgatcctga atctgcaccg gttcttgaac tgttcagaaa aaaactcttt gcctcagaag
      181 aaaaagaccc ggaagcattt gttctgagag aagaactgat cagacagtac aattatgatc
      241 ttgtcacagc agtcagaaag aaaaacgacc tggcagtcgc ctatatgagc ccggaaactc
      301 tggattcaga aatatccaaa aatgaagata aaatcatgaa aaaatggatc aaaagatccc
      361 tgaagatttt catcaaggct ctcggaaacg aaagatgttc cgttgagaca aaactgaagc
      421 tgatcgaaat cattcatctc tccaaatacg gcggacccgc aaaactgatt ccaaaggctc
      481 cttcacttgc caaaagagct gatgaactgt tttcaatcgc agaagctgaa aaggatacta
      541 agctgatcaa gaaaatcact gatttaggcg cgctgtacta caatcttgca ctggaaaaca
      601 aataatgctc gataaactgg gactcggcga tttaagattc caccgggagg gaaatcctgt
      661 tctgtctttt tttctgatga ctgttctttc ctttttccta tttgccgatc agaatctgat
      721 tgctccaaat cttaaaaaca tcggaagatc cctcggtctg cacacagaac aggacatcaa
      781 ctggtatctg ggcggactga taccggtcag tttttttgtg ctgggcggga tagtatccct
      841 gagcatgggg gttctgtctg aaaaatatcc cagaaaaatt cttttggtat cttccatatt
      901 gctcggagaa atcccctgct ttctgactgg atttgcagaa aactatactc agtttttcat
      961 tctgagaact ttgtgcggtt tcggactcgg aggcgtattt ccaatccttt tttccatggt
     1021 aggagattat ttctcagaaa aatccagagt gaccgcatct gcatatgtat ctcttgcaat
     1081 gggtcttgga gtaggaatcg gtcagatggc cggcggcatt ttcggagaat cagatccctt
     1141 gaacggatgg agacagagtt ttatatggct ctccgcaccc tcctttccca ttgcaggtgc
     1201 atattttctt ttctgcggcg aaccgaaaag aggagtcttt tcatcgggaa acaatgagca
     1261 taaactgaat ttttctgata taaaaaacat attcaaaaac agtacaaaca gagcacttct
     1321 tctgcaggga attccaggct gtgttccctg gggtgtattc tttgtatatc ttgtcgattt
     1381 ctatgaaact gtgtacggtc taggaaaaac agcttcttca ctctatgtga ctcttgcagg
     1441 gatcggtgtg atgatcggaa ttattttagg cgggattatc ggacagaaac tttacaacag
     1501 aagcagaaga ctgcaggcag tgtttgcaat gtcatgcatt cttctcggaa ttgttcctgc
     1561 ctatttcctg attcacggaa aatggatgag cggaactgtt ttttttccgg ttctgaacct
     1621 tcttacagga atagtgctca ccctccctct ttccaatctc aggtccattc taa
//
LOCUS       DAOQGJ010001007         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53154_length_1671_cov_2.644802, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001007 DAOQGJ010000000
VERSION     DAOQGJ010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53154_length_1671_cov_2.644802"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..>1671
                     /locus_tag="PK683_14495"
     CDS             48..>1671
                     /locus_tag="PK683_14495"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH09705.1"
                     /translation="MSKPLIVQSDKTLLVEVDNPEFEDCRSHISRFAELEKSPEYLHT
                     YRISPLSLWNAASSKLSADEIIGSLEKFARYSVPKNVINEIREQISRYGKVKLVMEET
                     GELVIISNEKGFIQEIGSHRSIQPFIQERLSTDKISVKKEYRGHIKQALIKIGFPVED
                     LAGYDEGNKYGFNLRPETLSGRKFGMRDYQRSSIESFHAGGSNEGGSGVVVLPCGAGK
                     TIVGIGVMQLIGAETLILVTNTLSIRQWKNEILDKTDIPESDIGEYSGETKEIKPITI
                     ATYNILTHRKKKGSEFTHFHIFSANNWGLIVYDEVHLLPAPVFRMTSELQAKRRLGLT
                     ATLVREDGLEEDVFSLIGPKKYDVPWKELESKSWIAEAKCVEIRVGMEDDLRLRYSIS
                     DDREKFRLASENPEKLKAIELVLEKHKGSNILIIGQYIDQLETIAGHFKIPLITGKTP
                     LGERQELYQAFRSGQIKSLVVSKVANFSIDLPDANVAIQISGTFGSRQEEAQRLGRVL
                     RPKPNGNQALFYSVITNESSEEQFCRNRQLFLT"
BASE COUNT          553 a          356 c          391 g          371 t
ORIGIN      
        1 aaatggttag caaaaaataa acaagcatta aactaaaagg agaatatatg agtaaacctc
       61 tgattgttca aagcgacaaa actcttcttg tggaagtgga caaccctgaa tttgaagact
      121 gcaggtctca tatttccaga tttgcagaac tggaaaaaag tccagaatat ctgcatacat
      181 acagaatatc ccctctttct ttatggaacg ctgcatcaag caaactcagt gcagatgaaa
      241 tcataggcag tctggaaaaa tttgcgagat actctgttcc taaaaacgtc atcaatgaaa
      301 tcagggagca gatcagcaga tatggcaagg taaagctcgt catggaagaa accggagagc
      361 tcgtcatcat ttcaaatgaa aaaggattca ttcaggaaat cggaagccac agaagcattc
      421 agcctttcat tcaggaaaga ctcagcactg ataaaatttc cgtaaaaaaa gagtacagag
      481 ggcatatcaa gcaggcactc atcaaaatcg gatttcctgt tgaagatctg gcaggatacg
      541 atgaaggaaa caaatacgga ttcaacctga gaccggaaac actgagcggc agaaaattcg
      601 gaatgagaga ttatcagaga tcttccattg aatcctttca tgcgggagga agcaatgagg
      661 gcggttcagg agttgttgta cttccctgcg gtgccggaaa gacaattgtc ggcataggtg
      721 tcatgcagct cataggtgca gagacgctca ttcttgtaac aaacaccctc tcgatcagac
      781 agtggaaaaa tgagatcctt gacaagacag acattcctga atcggacata ggcgagtatt
      841 cgggcgagac aaaagaaata aaaccaatta caatagcaac atataatatc ctgacccaca
      901 gaaagaaaaa gggatccgag tttactcact tccatatctt cagtgcgaac aactggggac
      961 tcatcgtata tgatgaggtg catcttcttc ctgctcctgt attcagaatg acgtcagaac
     1021 tgcaggcaaa gagaagactt ggactgactg caacgcttgt cagggaagac ggactggaag
     1081 aagatgtatt cagtctgatc ggtcctaaaa aatacgatgt tccctggaag gaacttgaaa
     1141 gcaagtcatg gatcgcagaa gctaaatgtg tggaaatcag agtgggaatg gaagatgatc
     1201 tgagactgag atattccatc tcagacgaca gagagaagtt ccgtctggca tcagaaaatc
     1261 ctgaaaagct gaaagcgatt gaactagtcc tcgaaaagca taaaggttca aacatcctga
     1321 tcatcggtca gtacattgat cagctggaaa caattgccgg acattttaaa attcctctga
     1381 ttaccggaaa gacgcctctt ggagaaagac aggaactgta ccaggcattc cgttccggac
     1441 agatcaagtc tctggtggta agcaaggtgg caaacttttc catcgacctt cccgatgcaa
     1501 acgtcgcgat acagatctca ggtactttcg gcagccgcca ggaagaagcg cagcgactcg
     1561 gccgtgttct gcgccctaaa cccaacggga accaggcgct gttctattcg gtgattacca
     1621 acgagtcgag cgaagaacag ttctgccgta accgccagct cttcctgacc g
//
LOCUS       DAOQGJ010001008         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53203_length_1670_cov_2.633437, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001008 DAOQGJ010000000
VERSION     DAOQGJ010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53203_length_1670_cov_2.633437"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..>1670)
                     /locus_tag="PK683_14500"
     CDS             complement(26..>1670)
                     /locus_tag="PK683_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09706.1"
                     /translation="NPEKMDKKIWKGNIRTDEFKEKNEEIIPVEKIPEFRLNISRELL
                     KSVKEARLTYIRPVRLFLTDISAEPSEAGNSEVQKKDYVNSVVKVEKIRLELFDLDGK
                     PVPSSTAAKSLGKVKKLKLDQNDGHLYFGNSYFIAFRYAKAPGNAEKLILKDENQWRT
                     AVLPVIQTGGKNLSPAEKQLLREAVYTSLLRVNKILTLPRTPYQKILEERNLKITEFA
                     GELSVNSVFVIKAEVKDSIWKLQTEIVSASESAVPGYSLNYDLNMEGKSLSSAASEWE
                     TVLTAFYGDPAIRSKTSPKTPIDFREDSAWEKYYQAKELYLSAATEENLYRSAELLKE
                     AATLDPEYSEALALQSLAKQKLALVLKSKGADYKSTEKIRKDGRELADKSAALSPNPS
                     AYRSLCIFFWNNRDRMMQFSSLALDGNGRDADAKFYFTRAAFQKKMYRLRPDSSELSQ
                     YYSWNPNSFAANHFMMELSLYHSAGDKAIDYMNKILKLNELSEIQKFNLQGNVFRAVK
                     KFDLAKANYSKALELSPGSPEANFGMGEISLAEGGKEKA"
BASE COUNT          395 a          384 c          331 g          560 t
ORIGIN      
        1 actgttcatc attttgattt tctcttcaag ctttttcttt tcctccttct gcaagggaaa
       61 tctcgcccat tccgaaattt gcttccggtg atccgggaga cagttccaga gccttgctgt
      121 agttagcctt ggccagatca aattttttga ctgctctgaa cacattcccc tgcagattga
      181 atttctgaat ttctgaaagc tcgttcagtt tcagaatctt attcatatag tcaattgcct
      241 tgtctcctgc tgaatgatac aggctgagtt ccatcataaa atgattcgca gcaaatgaat
      301 ttggattcca ggaataatac tgtgacagct ctgacgaatc aggtctgagt cggtacattt
      361 ttttctgaaa agcagctctc gtaaaataga attttgcatc ggcatccctt ccgtttccgt
      421 ccagagccag agaggaaaac tgcatcatcc tgtctctgtt gttccagaaa aaaatacaaa
      481 gcgatctgta tgctgaagga ttcggcgaca gtgcagctga cttgtctgca agttcccttc
      541 cgtctttcct aattttctcc gtggacttat aatcagcacc tttagacttt agaacaagtg
      601 caagtttctg ttttgcaagc gactgcagtg ccagtgcttc actgtattcc gggtcaagag
      661 tagccgcttc tttcagcagt tcagcagatc tgtacaggtt ttcttccgtt gccgcagaca
      721 gatacagttc cttggcctga taatactttt cccaggcaga atcctcccgg aaatcaattg
      781 gtgtcttagg agatgtcttg gatctgattg ccggatcgcc gtagaatgca gtcagaactg
      841 tttcccattc agaagctgca gagcttaatg acttgccttc catattcaga tcatagttca
      901 gactgtatcc gggcactgct gattctgatg cagatacaat ctctgtctgc agtttccata
      961 tactgtcttt tacttctgcc ttgatgacaa atactgaatt gaccgaaagt tcacctgcaa
     1021 attcagtgat tttcagattc ctttcttcca gaattttctg atatggtgtc ctgggaagag
     1081 tcagaatctt attgacccga agcagtgagg tatatactgc ttctctcaac agctgctttt
     1141 ctgcgggact caagtttttg cccccagtct gaatgaccgg cagaacggct gttctccact
     1201 ggttttcatc tttcaggatg agtttctcag cgttacctgg ggctttcgca tatctgaaag
     1261 caataaaata actatttcca aaatagagat ggccgtcatt ctgatccagt ttcagtttct
     1321 ttacctttcc aagcgatttt gcagcagtgg atgagggcac tggttttcca tccagatcaa
     1381 aaagctccag tctgattttc tcaaccttga cgacagagtt tacatagtct tttttctgta
     1441 cttctgaatt gcccgcttct gaaggctctg ctgaaatgtc agtcagaaaa agacgcacag
     1501 gccgaatgta cgtcagtctt gcctccttga ctgattttag aagctctctg gatatgttca
     1561 gtctgaattc aggtattttt tcgacgggaa tgatttcttc atttttttcc ttgaattcat
     1621 ctgttcttat attgcctttc catatttttt tatccatctt ttccggattt
//
LOCUS       DAOQGJ010001009         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53213_length_1670_cov_2.201858, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001009 DAOQGJ010000000
VERSION     DAOQGJ010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53213_length_1670_cov_2.201858"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PK683_14505"
     CDS             complement(<1..1071)
                     /locus_tag="PK683_14505"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH09707.1"
                     /translation="MIKHLWCNYILTIGQFFIKAQALICIFYGGNPVFAEEHMSSHLH
                     CTGKVKLMNIVECVLDHSPEYRTARLQVGVMQGRKKTSAYLFPSNPVFSFAYAHRKQA
                     GESSGISLGGTSSAVNGEIAVSQEIFISSQRRIRIGIAESDLSSSEKRAYVSERNTSA
                     DALSAAVRLSSADEEMEVSSELYSTAKNLYAVVQARAERGLLSAVEADLAKSELTRMT
                     RVLQSSKRKREAARANLIVMMGAPSFAEVKIEDSLPEISLKNKGTEQLIESALNYRME
                     IEAAVSDVRSAERRMELLRTERIPNLNLGLYLQRDGFNENVAGARLSFPLAAVRNYSG
                     EIEEAKHTAEQKRSSAEVARHTV"
     gene            complement(1106..1417)
                     /locus_tag="PK683_14510"
     CDS             complement(1106..1417)
                     /locus_tag="PK683_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09708.1"
                     /translation="MKKLLPSVFAVLLISLFLFQKTIDNEILCGDIGGVENTCPYSDS
                     DHESDHICINCPCNSLFSARPEMSVPDIQISFLTVSVPRELFPFPAFSLRSYLFRPPK
                     A"
BASE COUNT          438 a          387 c          348 g          497 t
ORIGIN      
        1 cactgtatgt cttgcgactt ctgcagaaga tcttttctgt tctgcagtat gctttgcctc
       61 ttctatttca ccggaatagt ttctgacagc cgccagggga aatgaaagtc tggccccggc
      121 cacattctca ttgaagccgt ctctctgaag atacagaccg agattcagat tgggaattcg
      181 ttcggttctg agaagttcca tccttctttc cgcgcttctt acatcggaga cagctgcttc
      241 aatttccata cggtaattca gtgcagattc aatgagctgt tctgttcctt tattcttcag
      301 agaaatttca gggagactgt cttcaatttt cacttccgcg aaactgggag cccccatcat
      361 cacaatcaga tttgcccttg cagcttccct ttttctcttt gaactctgaa gaaccctggt
      421 catcctggtc agttcggact ttgccagatc tgcctccact gctgaaagca gtccgcgttc
      481 cgctcttgcc tgaactacag cataaagatt ctttgctgta ctgtaaagtt cagacgacac
      541 ctccatttcc tcatccgctg agctgagcct cacggccgca gagagtgcat ctgcagaagt
      601 gtttctttcg gaaacgtatg ctcttttttc agagctgctg agatcggatt ctgcaattcc
      661 aatccgtatc cttctctgag aggaaataaa tatttcctgt gataccgcaa tctctccatt
      721 gaccgcagaa gaggttcctc ccagagaaat gccggaagac tctcccgcct gcttcctgtg
      781 agcatatgca aatgagaaga caggattgga ggggaacagg taggcagatg ttttttttct
      841 gccctgcatc actcccacct gaagtctggc cgttctgtac tcagggctgt gatcaagcac
      901 gcattccaca atgttcatca gcttcacttt gccggtacag tgcagatgag aactcatatg
      961 ctcctctgca aagacgggat ttccgccata aaaaatgcag atcagggcct gagcttttat
     1021 aaaaaactgc cctatcgtta aaatataatt acaccataag tgttttatca ttaatactct
     1081 cctttttttt ctgactcgga cagagtcagg ctttaggagg acggaacaga taggatcgta
     1141 aggagaatgc aggaaaagga aaaagttctc tcggaactga gactgtcaga aaactgatct
     1201 gaatatcagg gactgacatt tccggacgtg cactgaacag cgaattgcag ggacagttga
     1261 tgcagatatg gtctgattca tgatcagaat cagagtaagg acatgtattt tccactcctc
     1321 cgatatcgcc gcagagaatc tcattgtcta ttgtcttctg aaaaagaaaa agagaaatca
     1381 gtaaaacagc gaatacagaa gggagcagtt ttttcaaacc tttcagaaat gaaaaagtcc
     1441 taaaatgcaa attcattctc ccttttccaa tcataaataa agcgattatt tattccagaa
     1501 gaatttttca cagttcaggc cttgaaaacg gcagtaaagc gggtcttatt ttgaaggtat
     1561 ttttttggaa gtaagttcct tcactctttc tctgagcagt ttttcagtag ccttggcaag
     1621 attgacaatt tttgtcttgg tcatcttttc ctttgtgatc tcggctattt
//
LOCUS       DAOQGJ010001010         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53222_length_1670_cov_1.944892, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001010 DAOQGJ010000000
VERSION     DAOQGJ010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53222_length_1670_cov_1.944892"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PK683_14515"
     CDS             <1..912
                     /locus_tag="PK683_14515"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH09709.1"
                     /translation="IMQIGTVIELALLSLALADRYKFIQDENIRVHMENSVLERKAKE
                     ELEGRVKERTLELNKSLDLIQADLLMARKIQSYTLSSDVSKIQDLEIVIKYKAMNKVG
                     GDFYHIHRLTDRTVRIFLADATGHGVQAAMITMAIQGVYEGIKNYELPPDRILEIFNN
                     EFIRRYGQLNSFLTCILLDIHTDSGKIIYASAGHPSAFLLKETGFELLPKTGPLIGFI
                     KDHSYSTNEAEMDRNSRLYIYTDGIFEEFSDGQEFGEERLHQIFLKERNEKIESVISK
                     VFQNLQEFLNGTEFQDDIAVIGIKYRE"
     gene            complement(1023..>1670)
                     /locus_tag="PK683_14520"
     CDS             complement(1023..>1670)
                     /locus_tag="PK683_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09710.1"
                     /translation="ESVNPAKMPDRKVYFFSILAVLGILFFPPFYWIRKKRKNLVFSI
                     LSIIIISYFILFLGYTKSFEVQERYDFFSFILPFLLEGLKKERLIRAFKFFLYTGLAV
                     LYAMFFRYQFEIGKLDSPLRLYREAALQGKEFHFSSEDLPSYDRGRHFLAWLYLDHLK
                     IYNIGDKYLRLKELYSFSNPDEFDYSKKCKQADVFISQKMKDYLLKTKKIKCSSD"
BASE COUNT          569 a          327 c          323 g          451 t
ORIGIN      
        1 ataatgcaga ttggaacagt cattgagctg gctctgcttt cgcttgccct ggcggacaga
       61 tacaagttca ttcaggatga aaatatccgt gtccatatgg aaaactcagt tctggaaaga
      121 aaggcaaagg aagaactgga aggcagagta aaggaacgca ctctggaact caataagagt
      181 ctggatctga ttcaggcaga tcttctgatg gcaagaaaaa tacagtccta cactctaagc
      241 tctgacgttt ccaaaataca ggatctggaa attgtaatta aatataaggc aatgaacaag
      301 gtaggcggag acttttatca tatccacaga ctgaccgaca gaactgtccg tatattcctg
      361 gcagatgcaa ccggacacgg agtgcaggca gcaatgatca cgatggcgat tcagggggtt
      421 tatgaaggga tcaaaaacta tgagcttcct cccgacagga tactcgaaat tttcaacaac
      481 gaatttatcc gcagatacgg acagctgaat tcatttctga cctgcatact gcttgacatt
      541 cataccgatt caggaaagat catctatgct tcagcaggcc atccttctgc ttttttgctc
      601 aaagaaacgg gattcgaact tctccccaaa acaggccccc tgatcggatt cataaaggat
      661 cattcctata gtacaaatga agcggaaatg gaccgaaaca gcagacttta catatacacg
      721 gacggtatat ttgaagaatt ctccgacggt caggaattcg gagaagaaag acttcaccaa
      781 atctttctga aagagagaaa cgaaaagata gaatctgtca tttccaaagt ctttcaaaat
      841 ctgcaggaat tcttaaacgg aacagaattt caggatgata tcgccgtcat cggaataaag
      901 tacagagaat aattcactcg tttcaagctt tactgcattt atgcttgaaa tgaatctttt
      961 ttcttatggc ataatcttta cagcttaaga actctcacat cataagtaaa aaaaccttga
     1021 attcagtctg aactgcattt gatttttttc gttttaagca agtaatcctt catcttttgt
     1081 gatataaaaa cgtctgcctg tttgcatttt tttgaataat cgaattcatc cgggttggaa
     1141 aatgagtaca gttctttgag cctcaaatac ttgtcaccta tattatatat tttcaaatga
     1201 tctaagtaca gccaggccag aaaatgcctc cccctgtcat acgacggcag atcttcggaa
     1261 gaaaaatgaa attcctttcc ttgaagagcc gcctccctgt agagccggag aggagaatca
     1321 agttttccta tttcaaactg ataccgaaag aacattgcat aaagaacagc cagacctgtg
     1381 taaagaaaaa atttaaaggc ccttataagt ctttcctttt tcagtccttc aagcagaaaa
     1441 ggtaaaatga aggagaaaaa atcatatcgc tcctgaactt caaaggattt tgtatatccc
     1501 agaaaaagaa taaaatatga aataattatt atagacagaa ttgaaaaaac gagatttttc
     1561 cttttttttc tgatccaata aaaaggaggg aagaaaagga ttccaagcac tgctaaaatg
     1621 gaaaaaaaat aaactttccg atcaggcatt tttgccggat tgacagattc
//
LOCUS       DAOQGJ010001011         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53259_length_1669_cov_3.444857, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001011 DAOQGJ010000000
VERSION     DAOQGJ010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53259_length_1669_cov_3.444857"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="PK683_14525"
     CDS             complement(<1..1583)
                     /locus_tag="PK683_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH09711.1"
                     /translation="MSSSPKSKKDFLQKIDSSAGETKAQSRMDYAVFLESVNQFDDGV
                     EQINLIIKENPGLKHWQLTDIAYKLGMWKGHQETVSRNYAKALDWYNWAYSFRKNDPK
                     NKENWTLENLIDASSGALKTPLKKDAAVHRCAGVFIDKVSIHETSDGKRYDIENASTP
                     IMKERQILFQSVLRMIIERVTEGGMTLEFRNFDTDSIQLSLKGTDNLKPPLGELFQKL
                     TKEFDSIFPYFDGTGFTGGSWGGGTNYPIITEHYYSHLRGFVQHSASWVDSSAYQVLY
                     HEFFHSIEDIAGITPKHGFFPENRKHFPGWKGRDEYDYFKWHFETGLRKKPWTELNYL
                     KNQKNEEVWPDEFTSRVRSRLDALPYQNKKESGLLVRKGLEIWNQKKDELSASRLMNE
                     ALTKNPYRFEALKFLADRAYDKKEYSKAMKYYSQYNDIYYDPNIYLHIGHHYMWVVND
                     RAKAEEAYLSYLKQFPKTKEEGKFQVYLARVYEEMKDYSNAKKYYEKAYKENQDNAAV
                     PQGCFRLAYILNEVDGNKS"
BASE COUNT          424 a          379 c          301 g          565 t
ORIGIN      
        1 tcggacttat tgccgtccac ttcattcaaa atataggcca gtctgaaaca tccctgaggg
       61 acagctgcat tgtcctgatt ttctttatag gccttttcat aatacttttt agcatttgaa
      121 tagtctttca tttcttcata gactctggca agatacactt gaaattttcc ttcctccttc
      181 gttttgggaa actgtttcag ataggacaga taagcctctt ctgcttttgc acggtcattt
      241 acaacccaca tataatgatg acctatatgc agatatatat tcggatcata atatatatca
      301 ttgtactgac tgtaatattt cattgcttta ctgtattctt tcttatcata agctctgtcg
      361 gcaagaaatt tcagtgcctc gaaacgatat ggatttttag tcaatgcttc attcatcagt
      421 ctggatgcag acaattcatc ctttttctga ttccagattt ccagtccttt ccggacaaga
      481 agacccgatt cctttttatt ctgataaggc agagcatcca gtctgcttct gactctgctg
      541 gtgaattcat ccggccagac ttcttcattt ttctgattct ttaaataatt cagttcagtc
      601 caaggtttct ttctgaggcc agtttcaaaa tgccatttga aatagtcgta ttcatctctg
      661 cctttccacc ctggaaaatg ttttcgattt tccggaaaga acccgtgttt tggcgttatg
      721 cctgcaatat cttctatact gtggaaaaac tcatgataga gcacctgata agcggaagag
      781 tccacccaac tcgctgaatg ctgaacgaat cctcttaaat gagaataata atgctctgta
      841 atgataggat aatttgttcc tcctccccag gatcctccag taaagcctgt cccgtcaaaa
      901 taaggaaata tactgtcaaa ttccttagtc agtttctgga aaagttctcc gagaggcggt
      961 ttcagattgt ccgtgccctt caaagacagc tggattgagt ctgtatcaaa atttctgaac
     1021 tccagagtca ttcctccctc agtgactctt tcaatgatca tccgaagcac ggactgaaaa
     1081 aggatctgac gttctttcat aatcggggtt gaagcatttt caatgtcata ccttttcccg
     1141 tcggaggttt catgaatgga aaccttatca ataaaaactc ctgcacatct gtgaactgct
     1201 gcatcttttt tcaggggggt tttaagagcg cctgatgagg catcaatcag gttttccaga
     1261 gtccagttct ctttattttt cggatcattt tttcgaaatg aatatgccca attataccag
     1321 tccagtgctt ttgcataatt cctggatacg gtttcctgat gacccttcca cattccgagt
     1381 ttataagcaa tatctgtcag ctgccagtgc ttcagaccgg gattttcttt tataatcaga
     1441 ttgatctgct caaccccgtc atcaaactga ttcaccgatt ccaggaagac tgcataatcc
     1501 attctgctct gagcttttgt ttcacctgct gatgaatcaa ttttctgaag gaaatccttt
     1561 ttgcttttcg gactgctgct caatgcttcg gtactgttct gaccttttgg ctgattctcc
     1621 cgaattcctg aatctgctgt gcggcagttt tgaagaacaa gcaatacag
//
LOCUS       DAOQGJ010001012         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53272_length_1669_cov_2.671004, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001012 DAOQGJ010000000
VERSION     DAOQGJ010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53272_length_1669_cov_2.671004"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="PK683_14530"
     CDS             complement(<1..1330)
                     /locus_tag="PK683_14530"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNH09712.1"
                     /translation="MSFNELNSVENYIIYKLSGKNLNAEKPDSSKVFTAKSMDRKNLP
                     GQIGWVYKAGTELERTTEDIFVESELKEALVRINPEIKKNPDLAEEIIYKLRAVLISV
                     NHTGLVKANEEFSLWLKGEKTLAFGKNNQHIPIKIIDYTNIENNSFIVTNQYRVYSKE
                     TKIPDIVMLINGIPVAIGEAKTPIRPSVSWLDGASDIHDVYENAVPQLFVPNIFSFAT
                     DGKELFYGSVRCPLEHWTPWRIPDSENSLSYAIGLKEVGNELNDLLSPFRLLDILQNF
                     AIFSTDKKKRRIKVVARYQQYEGANRIVERVKFGSDKKGLIWHFQGSGKSLLMLFAAG
                     KLRRDAELKSPTVIILVDRVDLDTQITGTFNAADIPNLIATDSIKELHTLLEQDTRKV
                     IITTIFKFKDAKPNMNLRDNIIVLVDEAHRTQEGNLGRQMRAALPNAYLFG"
     gene            complement(1426..>1669)
                     /gene="rhuM"
                     /locus_tag="PK683_14535"
     CDS             complement(1426..>1669)
                     /gene="rhuM"
                     /locus_tag="PK683_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HNH09713.1"
                     /translation="AETMAQQRIPMYMKDWISRLDTILQLNGRELLTHAGQISHEMAL
                     HKSSAEYEKYLETQKKEQRENSLKELEEDVKKMGKK"
BASE COUNT          425 a          381 c          281 g          582 t
ORIGIN      
        1 gaccgaataa ataagcattc ggcaaagcgg cccgcatctg tcttcccaga tttccttcct
       61 gtgttctgtg agcctcatct acaagcacga tgatattatc cctaagattc atattgggct
      121 ttgcatcttt aaacttgaat attgtagtga tgattacttt tctggtatcc tgctccaaaa
      181 gagtatgcag ttctttgata ctgtctgttg cgatcagatt cggaatatcg gcggcattaa
      241 atgttcccgt aatctgcgta tccaaatcaa ccctgtccac taaaatgatc acagtcggac
      301 tttttaattc agcatctctc ctgagtttcc ctgcagcaaa aagcataagc aaagacttgc
      361 ccgatccctg aaaatgccag attaaacctt ttttatcgga tccgaatttt actctttcca
      421 caatccgatt agctccttcg tactgctgat aacgggccac tactttgatc cttcgcttct
      481 ttttatccgt gctgaaaata gcaaagttct gcagtatgtc gagtaatcgg aatggagaaa
      541 gcaaatcatt cagttcattc cccacctcct ttaaaccgat tgcataagag agggagtttt
      601 ccgaatcagg aattctccag ggagtccaat gctccaaagg acacctgact gatccgtaaa
      661 agagttcttt tccgtccgtt gcaaaagaaa aaatgtttgg aacaaaaagc tgcggaactg
      721 cattttcgta tacatcatgt atgtcagacg ctccatccag ccaggagaca gaaggtcgaa
      781 tcggtgtttt tgcttctcct attgcaacgg gaattccgtt aatcagcatt acaatatcag
      841 gaatcttagt ttctttagaa tatacacgat attgatttgt gacaataaaa gaattatttt
      901 ctatattcgt ataatctatt attttaatag gaatatgctg attgtttttt ccgaaagcca
      961 gagttttttc ccctttcagc caaagcgaga attcttcgtt agcttttacc agacctgtat
     1021 gatttacact gatcagaaca gctctcagct tatatatgat ctcttcggct aaatcaggat
     1081 tcttcttgat ttccggatta attcggacta aagcttcctt aagttcagac tcaacaaaga
     1141 tgtcttctgt tgttctttcc agctcagttc ctgccttata cacccatcca atctgcccag
     1201 gcagattttt tctgtccata cttttggctg taaatacctt actgctgtcc ggtttttctg
     1261 cgttcagatt tttccctgaa agtttatata taatataatt ttctacagaa ttcaattcgt
     1321 tgaatgacat ttttttcccc tttctcggct gttaagcttt tggcttttgg cttttggcct
     1381 ttggccttta gctcttagcc aaaagccaag cgctaacagc tatccctatt tcttccccat
     1441 cttttttaca tcttcttcca attctttcaa gctattttct ctttgttctt tcttttgagt
     1501 ttccaaatat ttttcatatt ctgccgatga tttatgaaga gccatctcgt gactgatttg
     1561 ccctgcatgg gttaatagct cccgtccatt caattgcagg attgtatcca gcctacttat
     1621 ccaatctttc atatacatag gaattctctg ctgtgccatt gtttctgca
//
LOCUS       DAOQGJ010001013         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53310_length_1668_cov_3.168010, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001013 DAOQGJ010000000
VERSION     DAOQGJ010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53310_length_1668_cov_3.168010"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1078)
                     /locus_tag="PK683_14540"
                     /pseudo
     CDS             complement(1..1078)
                     /locus_tag="PK683_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
BASE COUNT          456 a          399 c          305 g          508 t
ORIGIN      
        1 cattcttttc tttttcgttc agaagttcaa tttcagcatt ctttcttttt gtaatgtccg
       61 tgctgatgga aagaaactga gatacctgcc cgtaaatgtc aaatatcgga tttacaacgg
      121 tatccagcca gaattccctt cagtccctgc ctctgtttct gatctcattt ctccaggtcc
      181 tgccctgact gatcatatcc cacatttctg tccaaaattc gcggctgtga aatcctgaat
      241 taaaaatgct gtgatccctg cctaaaagtt cagcttcttc aaacccaaaa aggtcacaga
      301 gcttcctgtt ggcttttagg attctcccgt ccctgtctgc aatagtcaca agcgcactgc
      361 tgtcaagggc atccagaatg gaactcagct cctggttttt ttcagtgact gcctgcagag
      421 cgagaacccg ttcagtcgtg tctcttccgg tacactgaat tctgttcaga aatccttcac
      481 tgtcagtgac acctgtaaaa tcccattctg aatacataac cgttccgtca ggacagggct
      541 ttctgagaat gacgctgaaa acctgattcg gattctgcac acatttcatt acagtatcat
      601 gacattttcc atggtcctcc ggaatgatat gagtaagacc gctcattccc aaaatttcgg
      661 aaagttctat gttgaaaaca ttgcagaaat gcctgttggc atatgtatat cttccctgaa
      721 ggtcggtagt cagaaaaaag atgaactgcc ctgccagaat ggattcaaac ttttccactg
      781 agggcatata ctcggtcttc ttctgagccg ccttgaagat tgaattcgca cctagaattt
      841 tttcagtgaa gccttctttg tttctgaaaa gacggattct gctttttacc cagacgattt
      901 ctccgctttt atgaacatat ctgagcatct gttcgctcaa gtgttctgct gtttccggag
      961 ctctggaaaa aatatggaaa ttgacatctt tcccgtaaat cagagtcctg tgcacattag
     1021 gtgaattctg aagttctccc ggttcataac ctagagattt ccagaatgtt ccgttcatcc
     1081 attcctcgcc cgtaaaaatg tccaggaacc agatgcccgc actgccgttt tccagaacaa
     1141 attcaaggac agtttcatct ctgtgaatca gctcaaagaa actgttcttc aaaaaatctg
     1201 acataagaat tccgcgttgc tcctcagcat atacaatttt agctgcattc tcagacagtc
     1261 caataaaaat attttttcag cgtaaaataa tgcagcccgg cagaaataca atgcagtgaa
     1321 atgcacgcag agtcgtttta aaaccaggaa ttttgagctg ttttcagcat atcagagacg
     1381 tccggtataa aattaaactt aaagataatc aattttttta ctatccccgc ctgacagaca
     1441 gtcttccgaa aatgcagttt gcagtgaaac cgaattttga ggatcagttt aattattgaa
     1501 ataagatttt cgaagccgtc agcttttccg ccacggccct cagattgcat aatcaaaaaa
     1561 ccgggaaggc tctgaccttc cgtccagact caccattttc aggatgcaga atttccgagt
     1621 ttattcttaa aaactgattt caagcccgtc tctttctcat tttcatac
//
LOCUS       DAOQGJ010001014         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53318_length_1668_cov_2.760694, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001014 DAOQGJ010000000
VERSION     DAOQGJ010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53318_length_1668_cov_2.760694"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..549)
                     /locus_tag="PK683_14545"
     CDS             complement(214..549)
                     /locus_tag="PK683_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH09714.1"
                     /translation="MELIKREVNSIPIVDIVGEVDLYNTKEIKDLIDQMIKEGKYQII
                     LNLGQVPFMDSSGIGTLVTGMYRLKKYQGNLKIVNIVGSVAKVFKMTGMDAHLEIFET
                     EEDAVKSFK"
     gene            complement(676..1041)
                     /locus_tag="PK683_14550"
     CDS             complement(676..1041)
                     /locus_tag="PK683_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNH09715.1"
                     /translation="MNKKTSGTSVSEFGSISTNLGKDTYFNGTLTFKRPLQISGEFIG
                     EIISESFLYISDTAVVRANIQAHTVIVGGQVIGNVVATERLEMLSTGKVTGNIKTAKL
                     QIADGVVFDGNCEMIQPQK"
     gene            complement(1044..1265)
                     /locus_tag="PK683_14555"
     CDS             complement(1044..1265)
                     /locus_tag="PK683_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09716.1"
                     /translation="MISKEMKELLEFYNKGLQLYKEAKFQEALLEFQKGLGLIPDDGP
                     TLEYMKRCKEYIKEPPPADWDGVYVMKTK"
     gene            complement(1343..>1668)
                     /locus_tag="PK683_14560"
     CDS             complement(1343..>1668)
                     /locus_tag="PK683_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09717.1"
                     /translation="LDMIKIFGEITERWGIPVTIRVGIHTGSVVAGVIGKTKFVYDLW
                     GDAVNTASRMESHGMPGKVHCSESVQSRLQDKFSFEDRGIIEVKGKGPMHTFFVIGEK
                     NGKRF"
BASE COUNT          477 a          373 c          281 g          537 t
ORIGIN      
        1 tattggatgc agcggtgact tgagtcagat agtttgtatt tgcaggactg gaagctaaca
       61 gagaagaagc ctgatagagc ttggcatagg aatctgccgc agactgagac agaaggaatg
      121 caatcaaata attttgaata gggtcttttt tcttcttatt gatcagactg cattgtaagt
      181 gcaaacaaac cgcaataaca aaaagtagtc tcatcattta aatgatttca ctgcatcttc
      241 ttcagtttca aagatttcaa gatgagcatc catacctgtc attttgaaaa cctttgctac
      301 tgatccgaca atgtttacta ttttcagatt gccttggtat ttttttaaac ggtacatccc
      361 tgttaccaat gtcccaatcc cggatgaatc cataaaaggc acttgaccca gattcagaat
      421 gatttggtat ttcccctctt taatcatctg atcaatcaga tcctttattt ctttcgtgtt
      481 gtataagtct acttcgccga ctatatctac tattggaata gaattcactt ctctttttat
      541 cagttccatt tcaatctaac tgtcccacta ggtttttgat aacaagacac taaaatccct
      601 gtcttctatt acaaggaaaa atttatcata aaggaaaaaa attcttattt ccgcaaaaat
      661 agtttcagaa gaacactatt tttgaggttg aatcatttca cagtttccgt caaatacaac
      721 tccatctgca atctgcagct tggcagtttt tatatttcca gtgacctttc ctgtagacag
      781 catttccagc cgttcggtcg caacgacatt gccgatcacc tgaccgccta caattacagt
      841 atgcgcctga atatttgccc tgacgactgc tgtatcactt atatataaaa aactttctga
      901 aatgatttcg ccaatgaact caccgctgat ctgaaggggt cttttgaaag tcagtgtccc
      961 gttgaagtaa gtgtcttttc caagattagt agaaatagat ccaaattcag atactgacgt
     1021 acctgaagtt tttttattca tcatcatttt gtcttcatca cgtaaacccc gtcccagtca
     1081 gcgggaggcg gttcctttat atattccttg cagcgtttca tatactcaag agtaggaccg
     1141 tcatccggaa tcagaccaag tcctttttga aattcaagaa gtgcttcctg aaatttcgct
     1201 tctttgtaaa gctgaagacc cttgttataa aactcaagca gttctttcat ttctttcgaa
     1261 atcataattt gttaatttca gcgatcaagt aaaactggca agcaaaaaaa tgagaccctt
     1321 ttctgaacag taaacggcaa ggttaaaacc tctttccgtt cttctctcca atcacaaaaa
     1381 atgtgtgcat cggtcccttt cctttgactt caatgatgcc tctgtcttcg aaactgaatt
     1441 tatcctgaag tctggattgc acgctttccg aacagtgaac cttgcccggc attccgtgag
     1501 actccattct ggaggcagta ttgactgcat ccccccataa atcgtaaacg aattttgtct
     1561 ttccaatcac ccctgcaacc acagagccgg tatgaattcc tactcgaatt gtgactggga
     1621 taccccatct ttcagtgatt tcaccgaaaa ttttgatcat atccagtc
//
LOCUS       DAOQGJ010001015         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53324_length_1668_cov_2.543707, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001015 DAOQGJ010000000
VERSION     DAOQGJ010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53324_length_1668_cov_2.543707"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK683_14565"
     CDS             <1..252
                     /locus_tag="PK683_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09718.1"
                     /translation="KDEIRLPFGVSEVYYPEEIEYICANEDVHLFSDLVFRRSGIGNS
                     GRLPEAEFKKLLGFASKHFGGRIQERDIREVKSRYVLPV"
     gene            301..1371
                     /locus_tag="PK683_14570"
     CDS             301..1371
                     /locus_tag="PK683_14570"
                     /inference="COORDINATES: protein motif:HMM:NF022470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HNH09719.1"
                     /translation="MTDNRSFIVNSKNRNQYGLKNTGLKILSDNEITAKVKKFGFTSN
                     NVTYAALAETIPYLKFFPDASSEENGILPVWGIAEVTESKHSDIRPGQRFYGFLPAAE
                     YVQIRPASANSSGFTAERPQIRPEYGFYNACSFLEKDVFYNEVIEDEMTVFRPLFMTG
                     ILVADWLEFNEYRSADTVVISSASSKTSYGIAKAVKSNEKKCRLIGLASERNTAFCRS
                     LDVYDEVVSYDHPEGISSSYSVLYVDVAGNESLRNSLSASLGERLKLVLSVGMSHGST
                     DGFQAGTLGERFEVFFAPGWMMKRRRKLPEKIGSVWKAQISSVGKYFQTVRKNGIENF
                     EKDYADFLSGKINPSESILFQL"
     gene            1441..>1668
                     /locus_tag="PK683_14575"
     CDS             1441..>1668
                     /locus_tag="PK683_14575"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004496801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNH09720.1"
                     /translation="MSLCEKDDLYRSYHDQEWGFPVHDDRKFFEKLILDGAQAGLSWY
                     TILSKRENYRKAFADWDVHKVARFTERKMEKL"
BASE COUNT          513 a          305 c          386 g          464 t
ORIGIN      
        1 aaggatgaaa tcaggctgcc tttcggtgta tctgaggtgt attatcccga agagattgaa
       61 tatatctgtg ccaatgaaga tgtgcattta ttttctgatc tggttttcag aagatcaggg
      121 ataggaaatt cagggagact gccggaagca gaatttaaaa agctgctcgg ctttgcctca
      181 aaacatttcg gaggcagaat acaggaaagg gatattcggg aagttaaatc cagatatgtt
      241 cttcctgtct gatctgctgt tttttattta ctgaattaaa caaattattt acatagtctt
      301 atgacagata atcggtcctt tattgtaaat tctaaaaatc gaaaccagta cggtcttaaa
      361 aatacaggcc tgaagattct ttcggataat gaaattacgg ctaaagtgaa aaaattcgga
      421 tttacttcga ataatgtcac ttatgcagcc cttgctgaaa caatcccata tctgaaattt
      481 tttcccgatg catccagcga agaaaatggg atacttcctg tatgggggat agcagaagtc
      541 actgaatcta aacattccga tatcagacct gggcagagat tttacggatt tctgcctgca
      601 gcagaatatg tgcagatcag accagcttct gcaaacagct caggatttac tgcagagaga
      661 cctcagatca gaccggaata cgggttctac aatgcctgca gttttctaga aaaggatgtt
      721 ttttataacg aggttattga agatgaaatg actgtttttc ggcctttgtt tatgacaggg
      781 atacttgtcg cagactggct ggaattcaat gaatacagat ccgcagacac ggtggtcatt
      841 tcttctgcat cgagcaaaac ttcctacggt attgctaaag cggtaaaaag taatgaaaaa
      901 aaatgcagac tgatcggtct tgcatcggaa agaaatactg cattctgcag aagccttgat
      961 gtatacgatg aagtcgtttc ctacgatcat cccgaaggaa tttcttcttc gtacagtgtt
     1021 ctgtatgtgg acgtcgccgg aaatgagtct ctcagaaaca gtctgtcagc gagtcttggg
     1081 gaaaggctga agcttgtgct ttccgtagga atgtctcacg gcagtacaga cgggtttcag
     1141 gccggaacac tcggtgaaag atttgaagtt ttttttgctc ccggatggat gatgaaaaga
     1201 agaagaaaac tgccggagaa aataggatct gtctggaaag cacagatcag ttccgtcgga
     1261 aaatattttc agactgtcag aaaaaacgga attgaaaact ttgagaaaga ttatgcagac
     1321 tttctttccg gaaaaatcaa tccttccgaa tcaattctct ttcagctctg aattccctct
     1381 ttcgcaggcg gttataatga atactgaaga tggagcaggc agagcttcgg gaaaaatcag
     1441 atgtccctgt gtgaaaaaga tgatttatat agaagttatc atgatcaaga atggggattc
     1501 cctgttcatg acgacagaaa atttttcgag aagctcattc tggacggagc tcaggccgga
     1561 ctgagctggt acacaattct ttccaaaaga gaaaactaca ggaaggcttt tgccgactgg
     1621 gatgttcaca aggtcgcgcg ttttaccgaa aggaaaatgg aaaaactt
//
LOCUS       DAOQGJ010001016         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53327_length_1668_cov_2.497210, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001016 DAOQGJ010000000
VERSION     DAOQGJ010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53327_length_1668_cov_2.497210"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..>1668
                     /locus_tag="PK683_14580"
     CDS             109..>1668
                     /locus_tag="PK683_14580"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNH09721.1"
                     /translation="MSKNVKSLGKFYLISDLPLFSSLSSSDKAFIADRAQISEYKRGD
                     VIYEISAPKDFLYLIIQGTVELYLPEESAKGQKETPVQIMRKGDCLGIVSMLGSRPHS
                     LSARSLSDTRVIRFDDRSLSQILSRVPSLGLAFSRSLSKRMKVIQGSDGKIVESTVIA
                     VYSPDDEDFGSEYAADLCLQISKESARKAVVLRFNKKNKNFARSSLKVKEINSKSSED
                     LLNCLGTFLFNYHYIIMDLSPDNPDEVGLLLKEADSCHVLNLKETGLNYTQELLKNLK
                     MDPKKIAPSFFQILPLGRSWGSEKRSEYVRQKAREITGMRIGLSLGGGAAFGLAQIGV
                     LKVIEQEKILVDIVSGTSIGSLIGALWANGLSASDMEKMTEEFDSLFKMMKLMDFSIF
                     PKKGLVSGKNVRKFLSGFLGEQTFAELKRELRVVSCNINRREEIVISEGNVVEAVMAS
                     TAIPGLFNPIQKEDGRVLVDGGVVNPLPVSVLAKEGIQRIIAVNAMPSPDDVTKSNQQ
                     NQNLMDIFINSF"
BASE COUNT          471 a          321 c          402 g          474 t
ORIGIN      
        1 gattgcggag gcctgtgttt taaccttttt ttcaggctgc ctctttctgt aaactaggtg
       61 tgggaaaaaa ctgctttcca attctgcagt ctctggaaaa ttttcatcat gagtaaaaat
      121 gtcaaatcct taggtaaatt ctatcttata tccgatcttc ctcttttcag cagtctttcc
      181 agttcagaca aagcattcat cgctgaccgg gcacagattt cggagtataa gagaggagat
      241 gtaatttacg aaatttcggc tcctaaggat tttctttatc tgatcattca agggactgta
      301 gaattgtacc ttcctgaaga gagtgcaaag gggcagaagg aaacacctgt tcagattatg
      361 agaaaaggtg actgccttgg gattgtatcg atgctcggca gcagaccgca cagtctatcc
      421 gccagatcac tttccgatac aagggtcatc cgttttgatg acaggtcact cagtcagatt
      481 ttaagccgtg ttccttccct gggacttgcc ttttcgcgtt ctctttccaa gagaatgaaa
      541 gtcattcagg gaagcgacgg taaaattgta gagagtacag tcattgctgt ttactctccg
      601 gatgatgaag atttcggatc tgaatatgcc gccgatctct gtctgcagat ttcaaaggag
      661 tctgcaagaa aagcagttgt tctgcgattt aacaaaaaaa acaaaaattt tgcacgttct
      721 tctctaaagg taaaggaaat caattccaaa agttctgagg atcttctgaa ctgtctgggc
      781 acctttctgt ttaattatca ttatattatc atggatctct ctcctgacaa tccggatgaa
      841 gtcggtcttc tcctgaagga agctgattcc tgtcatgttc tgaacctgaa agagacagga
      901 ctgaactata ctcaggaact tctgaaaaat ctgaaaatgg accccaaaaa aattgccccc
      961 tctttttttc agatccttcc tttgggccgg agctggggat ccgaaaagag atcggaatat
     1021 gtgagacaga aggccaggga aatcacagga atgagaatag gactttctct cgggggaggg
     1081 gcggcattcg ggcttgctca gataggcgta ttgaaagtca ttgagcagga aaaaattcta
     1141 gtggacattg tttccggaac cagtattgga tcattgatcg gagcactttg ggcgaatggg
     1201 ctttctgcat cggatatgga gaaaatgacg gaagaatttg attccctgtt caaaatgatg
     1261 aaactgatgg atttttctat tttccctaaa aaaggacttg tttccggcaa aaatgtccga
     1321 aaatttttgt cgggtttttt aggagaacag acttttgcgg agttgaaaag agagctgaga
     1381 gttgtttcct gcaacatcaa cagaagggaa gaaattgtga tttctgaggg taatgtggtg
     1441 gaggcagtga tggcaagcac agcgattccg ggtcttttca atcctattca gaaagaagac
     1501 gggcgtgtgc ttgtggacgg aggggtagtc aacccgctgc cggtgagtgt actggcaaag
     1561 gaaggaatcc aaagaatcat tgcagtcaat gccatgcctt ctccggacga tgtgactaag
     1621 tcaaatcagc agaatcagaa tctgatggat atattcatca acagcttt
//
LOCUS       DAOQGJ010001017         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53429_length_1666_cov_2.662942, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001017 DAOQGJ010000000
VERSION     DAOQGJ010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53429_length_1666_cov_2.662942"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK683_14585"
     CDS             complement(<1..594)
                     /locus_tag="PK683_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09722.1"
                     /translation="MREVIRDIFAEIAPWIGENKILILRGSRQVGKSTLLRHIEEGLI
                     KRREKFFSYSIDQELDNPLFKSPSNLKNFFLDQSDNHFFYLILDEFQYLNDSGLFLKA
                     LHDSLKGKCQIIVSGSSSLEISKSTEFLTGRKIEFRIETFSFREFLKGNSEWKFDRKF
                     SLSSFKEAEEFDSIYSQELKNQFAEYLNFGGYPEIALE"
     gene            complement(786..1406)
                     /locus_tag="PK683_14590"
                     /pseudo
     CDS             complement(786..1406)
                     /locus_tag="PK683_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010579161.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
BASE COUNT          433 a          366 c          341 g          526 t
ORIGIN      
        1 ttccaaggct atttcaggat aacctccaaa gttcagatat tctgcgaatt ggtttttgag
       61 ttcttgtgaa tagatgctgt caaattcttc cgcttcttta aaactgctca gtgaaaattt
      121 tcggtcaaac ttccattcgg aattcccttt taaaaattcc ctgaagctga atgtttctat
      181 tctgaattca attttccttc ctgtcagaaa ttcagttgat tttgaaattt ccagactgga
      241 agagcctgat acaataattt ggcattttcc tttcaggctg tcatgcaaag cttttaaaaa
      301 cagtccagaa tcattcagat actgaaattc atccaaaatt aagtaaaaaa aatgattatc
      361 agactgatcc aggaaaaaat tttttaaatt ggatggactt ttgaacagag gattgtccag
      421 ttcctgatcg attgaataag aaaagaactt ctctcttctt tttatgagtc cctcttctat
      481 atgtctgagc aaggtgcttt ttccgacctg tctggaacct ctcaagatca gaattttgtt
      541 ttctccaatc catggagcga tttccgcaaa aatatctcga attacctctc tcataagctg
      601 agtaaatcag gcaggtgaga aaaatcaaga gattttcaca tacttagact aggaaaaaca
      661 aggaaaatca tcacttcaag gtgagaaaaa tcaagagatt ttttagaaac agggtacaga
      721 gaaactaggg agaaaaggtt gaatgacttg gttttgggtt tgcttttacg cagtcccttt
      781 aaatatcatt ccagtttcat acagaccttt ccgaaatgac ttcctgcttt caattcaccg
      841 aatgcctttg catattcttc gaagggatat actttatcta tcacaggttt tattttatgt
      901 gcagatactg ctttgcacat atcttcgaaa gatgttctgc ttccgacaag caccccttga
      961 attcggatat ttcccattaa gacaggtgtg agatttatat tggcatcgta tcctgccaaa
     1021 actccgatga gactgatatg tccgaacaga cgcactgcag tcattgactc ttccagtgtg
     1081 ccagggccgc ccacttcaat gatatgatca gcaccgttca ttccggtgat tttgcggact
     1141 tcttttcccc agttcggctt cgttttataa ttgatcagat aatctgcacc cagagatttt
     1201 gctctctgca gtttttcgtc gctcgaagag gtgacaatga ctgtggcccc agccatcttt
     1261 gcgaactgga gagctgctat ggaaactcct cctgttccct gcacaacgat gatgtctccc
     1321 ggcttgattt ttgtctctac aaagagcgcg gaccaggcag tcagggccgc acagggcagg
     1381 gtggcggctt cctcatcgga aaggttgctg tcaatgcttg ccatctggcc caggcgcttt
     1441 tgaacctggg caagctgtct taacagccct gtttgttctc catcatcgcc ttgcaataaa
     1501 aatgtgaccg cttgagcctc ttgtatcagt gtttggacct gttgcaaaac gttgagctgg
     1561 tttcggcagt gctcgtcctc gtttggatcg ctgaggtcgg ctgcctgaat gtcttgcagc
     1621 tgaaactggt agaggtcctg cttttccttg gcggcctgaa tttctg
//
LOCUS       DAOQGJ010001018         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53438_length_1666_cov_2.445686, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001018 DAOQGJ010000000
VERSION     DAOQGJ010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53438_length_1666_cov_2.445686"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..756)
                     /locus_tag="PK683_14595"
     CDS             complement(211..756)
                     /locus_tag="PK683_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HNH09723.1"
                     /translation="MSDIVKVSKFLSFILRHSPESIGIVLDEHGWADISEIIQKSSLN
                     LNFEIIEKTVQENNKKRFSLSPDRKKIRASQGHTIQTDVELKKEIPPEFLYHGTAEKH
                     LELILKEGLKPMKRLHVHLSKDIETALSVGRRHGKPSVLKIHSGRMYEDGFVFYLSEN
                     SVWLTDSVPVQYIERISTAFP"
     gene            complement(757..>1666)
                     /locus_tag="PK683_14600"
     CDS             complement(757..>1666)
                     /locus_tag="PK683_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNH09724.1"
                     /translation="PGKAHPLAILSTMVMALSSYYTDDYEESADRGVDHVTRLLAKVR
                     TIAAFSYKKMIGQPFVYPLDRLPYCTNFLHMVFSVPAEKYEVDPYFDKILNQLWILYA
                     DHEQNVAATTVQLVGSTKANLFASVSAGISALWGSREGGQSVAAVELIEDINRNGGDC
                     KAYFEKLKGSEIITQSTFLGHAAYKTVSPRAIAAREVFHEFSRKYRNPVLETALKVEE
                     YLINEPYFKERNLYPNLEFYSGVLFFSLGIPKNMFTLMQAIGKLPGWMAHWRELRINA
                     NYKKARPRQIYKGEMNRTYSPIEDRK"
BASE COUNT          420 a          372 c          347 g          527 t
ORIGIN      
        1 tcggaagctg gctcggcggc tttgaaactg cctaaatccc tatttagctt ctgattgaag
       61 ataatttctt gattttttca acaactccat tcaaaatata tctgctgata ctatgaaaaa
      121 tgcagatatt tccttctcac cttcagaagg actggagttt atattcatat gagtagtatc
      181 cgcataagcg ggtcatttat atttcagaat ttacggaaaa gcagtgctga tccgttcaat
      241 gtactgaact ggaacagagt ctgtcagcca gactgaattt tccgagagat aaaatacgaa
      301 tccgtcctca tacattctgc ctgaatgtat tttcagaaca gaaggttttc cgtgtcttct
      361 gcctacgctg agtgcagttt caatgtcctt ggaaagatga acatggagtc ttttcatagg
      421 cttcagtcct tccttcagga tcagttccag gtgcttttcc gctgttccat ggtacagaaa
      481 ctctggaggt atttccttct taagctccac atccgtctgt attgtatgtc cctgactggc
      541 tctgattttt tttcgatccg gactgaggga aaaccttttc ttattgtttt cctgtactgt
      601 tttttctatg atctcaaaat tcagattcag agaggatttt tggatgattt cacttatgtc
      661 tgcccagcca tgttcatcaa gcacaatccc gatgctttcg ggactgtgcc tgagaataaa
      721 gcttagaaac ttgctgacct ttacaatatc agacacttac tttctgtctt caataggaga
      781 gtaggttctg ttcatttctc ctttgtagat ctgacgcggt ctggcttttt tgtagtttgc
      841 attgattcga agttctctcc agtgtgccat ccagccggga agcttgccga ttgcctgcat
      901 cagcgtgaac atattcttcg gaattcccag actgaagaaa agcaccccgc tgtaaaattc
      961 cagattagga tacagatttc tttccttgaa atacggttca ttgatcagat attcttcgac
     1021 tttcaaagca gtttccagaa caggattcct gtattttctg ctgaattcat ggaacacttc
     1081 tctggctgca attgctcttg gactgacagt cttgtatgca gcatgtccaa ggaacgtgga
     1141 ctgagtgatg atttctgaac ctttgagttt ttcaaaatag gccttgcagt ctcctccgtt
     1201 tctattgatg tcttcaataa gttccaccgc agcgacactc tgtccgcctt ctctggagcc
     1261 ccagagtgct gagatccctg cactgactga agcaaacagg tttgcctttg tggaaccgac
     1321 cagctgaacc gtagtcgcag caacattctg ctcatgatct gcataaagaa tccagagctg
     1381 attcagtatt ttgtcaaagt atggatccac ttcatatttt tcagcaggga cagagaaaac
     1441 catatgcagg aagttcgtgc agtagggcag tctgtcgaga ggatagacga agggctgacc
     1501 aatcattttc ttatacgaaa atgccgcaat cgttcttacc ttagcgagaa gtctagtcac
     1561 atgatcgact cctctgtccg cagactcttc gtaatcgtca gtataataac tcgaaagtgc
     1621 catcaccatt gtggaaagaa ttgcaagagg gtgtgccttt ccggga
//
LOCUS       DAOQGJ010001019         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53464_length_1665_cov_3.636646, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001019 DAOQGJ010000000
VERSION     DAOQGJ010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53464_length_1665_cov_3.636646"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>1665
                     /locus_tag="PK683_14605"
     CDS             76..>1665
                     /locus_tag="PK683_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09725.1"
                     /translation="MSTEILDQLAVGIIIIDLDEKIRYANANLKSHFLNSGFNPSGKK
                     INDPTMPWYTGLKNPREKLPILKAKLTGNTINEITEIKIKNETVKVVNIAVPLRNPKG
                     ILIGAVGLFLNAGLFQPSSSSDEVLQKLYAVLKRFISNNTLSHIVSSLKSAPPPAEKC
                     FVTIAFIDIVGFSTLAETLEPHQTVNVLNLFFGRVHKTIARYKGDIDKYLGDAVLSVF
                     FNAEACVRCCTELLLSDLPVINGQLRKLFPNISDLGVHIGINSGWVILGEVGAAERKE
                     FTVIGDDVNTAARIQSLTPPNEIWISGQTMDKLGEFNRLMKEAEYIKVKGKSAQIKVY
                     KFDPTQIKMDTKVFFYEKNEAYRNTISDALKNRGVTSITHSDSLENAEKVLNPQFESV
                     IIGPGSNANELLSIQKIVQKAGMGNEVLIPVSKDTDPKTIEALEKRGIKTYVPLTEGK
                     EFETNLENIIKSQKINKIPMKKELNETDSFVHSRLLKREDPEKMHTETEEPGASAREG
                     EESLSNSIIYSKQSDSLMITIM"
BASE COUNT          580 a          329 c          341 g          415 t
ORIGIN      
        1 gaaacaaaaa tgctttacca cattcagatt atgaattcac ttacatatag caattttgaa
       61 aaggcagtta cataaatgtc cacggaaata ctggatcagc ttgcagtcgg cattataatc
      121 attgatctgg atgaaaaaat tcggtatgca aatgcgaatc tgaaaagtca tttcctgaat
      181 tcaggattca atccctccgg caaaaaaatc aatgatccga ccatgccctg gtacactggg
      241 cttaaaaatc ccagggaaaa acttccaatc ctgaaggcaa aactcaccgg aaatacaatc
      301 aatgaaatca ctgaaattaa gataaaaaat gagactgtaa aagttgtgaa tatcgcggtt
      361 cctctcagaa atcccaaagg gattctgatc ggtgcagtcg ggctgtttct gaatgccggt
      421 ctttttcagc ccagcagttc atccgatgaa gtactgcaga agctttacgc cgtgctgaaa
      481 aggttcattt caaacaatac actgagtcat attgtttcca gcctgaaatc agccccgcct
      541 cctgcagaaa aatgtttcgt gacaattgca tttattgata tcgtcggctt ctctacactc
      601 gcagaaacac tggaacctca ccagacagtc aatgtactga atcttttctt cggcagagta
      661 cataaaacaa tagcaagata caagggagac attgacaaat atttaggaga tgccgtactt
      721 tcagtatttt ttaatgcaga ggcatgtgta cggtgctgta ctgaacttct tctttcagat
      781 ctgccggtaa taaacggaca gctcaggaaa ctgtttccta acatatccga tctgggcgtt
      841 catatcggaa tcaattccgg ctgggtaatt ttaggagaag tcggtgcggc agaaagaaag
      901 gaatttactg tcattggcga cgatgtgaat actgctgcaa gaatacagtc actgacaccc
      961 ccgaacgaaa tctggatttc cggtcagacg atggacaaac tcggcgagtt caacagactg
     1021 atgaaggagg cagaatatat caaagtaaaa ggtaaatctg ctcaaatcaa agtgtacaaa
     1081 tttgacccga ctcagattaa aatggatact aaagttttct tctatgaaaa aaatgaagct
     1141 tacagaaata caatttcgga tgctctgaaa aacagaggag tcacatccat cactcattca
     1201 gattcacttg aaaatgcaga aaaggttctg aatcctcagt ttgaatcagt cattatagga
     1261 ccaggttcaa atgcaaatga actgctttcc attcagaaaa ttgttcagaa ggccggaatg
     1321 gggaatgagg ttctgattcc tgtatccaaa gacactgatc cgaaaacgat agaggctctt
     1381 gaaaaaagag gcatcaagac atacgtacct ctgactgaag gaaaagagtt tgaaacaaac
     1441 ctggaaaata ttataaaatc acagaaaata aataaaattc cgatgaaaaa ggaactgaat
     1501 gaaacagatt cctttgttca ctcaagactt ttaaaaagag aagatcctga gaaaatgcat
     1561 actgagacgg aagagccagg agcctcagcc cgggaagggg aagagagtct aagcaattcc
     1621 atcatataca gcaagcagtc tgacagtctg atgattacaa ttatg
//
LOCUS       DAOQGJ010001020         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53527_length_1664_cov_3.165320, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001020 DAOQGJ010000000
VERSION     DAOQGJ010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53527_length_1664_cov_3.165320"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PK683_14610"
     CDS             complement(<1..179)
                     /locus_tag="PK683_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH09726.1"
                     /translation="MIKIFGLNKSYGKQVLFEDLGFSINRGEKIGLVGRNGHGKSTLF
                     QMILGNVEYDSGTISA"
     gene            465..1055
                     /locus_tag="PK683_14615"
     CDS             465..1055
                     /locus_tag="PK683_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09727.1"
                     /translation="MILGHYASALIPYSRFKKYPFWILLLSANVPEFLWLILAFSGVE
                     KTIPASLSDATFQNLKVNMIYSHNLVPAFLQGTAVGVLILSVFRDRSFAFWCGFLAFF
                     HVLCDLVVGFEHQVLGADSPVVSLNSYFLFPHAAILIECAFSFACVFWYIHTEKKQGM
                     ALTKRKIVYLFLFFGIGVLMWMPNATVPLKDILPFF"
     gene            1146..1574
                     /locus_tag="PK683_14620"
     CDS             1146..1574
                     /locus_tag="PK683_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09728.1"
                     /translation="MKNLFLICSLFFCLALHCSDNRQEKGVTLEEKLFSNSKDETIFS
                     KNGFAFYQKYIFKEGKLENFEYFGEVQFKTETGTTRTCVLFNITMTVEKKQYHHSFQI
                     EFQNEHIESGFWYYFPLDSQHQRYYAHSENFLEETGFLRK"
BASE COUNT          461 a          317 c          333 g          553 t
ORIGIN      
        1 gcactgattg ttcctgaatc atattccaca tttcccagaa tcatttggaa cagtgtggac
       61 tttccgtgtc cgtttcttcc caccagtcca attttttcac cgcgattgat gctgaaaccc
      121 agatcctcaa aaagaacctg tttgccgtat gatttgttca ggccgaatat tttaatcata
      181 aaatccagat ttttataagt gattttccaa tcaatgaatc tatgaattaa ttttcaggct
      241 cagtatgaat gaacggaaat ttttccggaa cagaaagcat tcactgtatc cgcttcggca
      301 aaggggaaaa atttttgacg aaatacagtc ttttgctttc ctattttttg cttaggtaaa
      361 taatcacgct ggaaggtgaa tatgtcctct cttatcgtca cgctgacttt attttatatc
      421 ctgggattct acctcgtcaa taaaatcgtt acaaaggatg atttatgatt ctaggacatt
      481 atgcctctgc cctgattccg tacagcaggt tcaaaaaata tcctttttgg attcttctgc
      541 tctccgcaaa tgtgcctgaa tttctctggc tgattctggc attttcgggt gtagaaaaaa
      601 cgatccccgc ctccctgtcc gatgcaacat ttcagaacct gaaggtgaat atgatttaca
      661 gtcataatct tgttccggcc tttctgcagg gaactgctgt cggagttctg attctttctg
      721 tcttccggga ccggtcgttt gctttctggt gcggatttct ggccttcttt catgtgttat
      781 gtgacctggt tgtgggattt gagcatcagg ttctgggtgc cgattctccg gttgtttcac
      841 tgaattctta ttttcttttt cctcacgcag ccatactgat tgaatgtgca ttcagttttg
      901 cctgcgtatt ctggtatatt catacagaaa agaagcaggg tatggcactt actaagcgca
      961 agatagtata tctattttta ttttttggga ttggagtctt aatgtggatg ccgaatgcga
     1021 cagtgcctct gaaagacatt ctgccgtttt tctagagttg atgacattga cgggaaggaa
     1081 ctggaaagca tcgagtataa atttgaaaac tgtccatcag acaagaaata ggagcagtga
     1141 agattatgaa aaatctcttt cttatatgtt ctctattttt ctgtcttgct ttgcattgtt
     1201 cagataatag acaggaaaag ggtgtaacat tagaggaaaa gctgttcagc aattccaagg
     1261 atgaaaccat tttcagtaaa aatggtttcg ctttctatca aaaatatata ttcaaggaag
     1321 gcaaactgga aaatttcgaa tattttggtg aggttcagtt taaaactgag acaggtacaa
     1381 cgagaacctg cgtactgttt aatattacaa tgacagttga aaaaaagcaa tatcatcata
     1441 gttttcaaat agagtttcag aatgaacaca ttgaatctgg attttggtat tattttcctc
     1501 ttgacagtca gcatcaaaga tattatgccc actctgaaaa tttcctggaa gaaacaggat
     1561 ttttaagaaa gtaatttttg aaacaagtca ttccgagaaa ggcgagcagc ggatttttct
     1621 tttctttcaa accgccaacc tgaaggttgg ctgcccaacc gccg
//
LOCUS       DAOQGJ010001021         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53607_length_1663_cov_2.962065, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001021 DAOQGJ010000000
VERSION     DAOQGJ010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53607_length_1663_cov_2.962065"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1381)
                     /locus_tag="PK683_14625"
     CDS             complement(140..1381)
                     /locus_tag="PK683_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09729.1"
                     /translation="MIVQLRLHTSAVIFVDLFKAEITLHSMSEFGKENILLKADSSEK
                     PAEKRKEFLALHLEIQEKGSADLEKRIRLKKLAAELLEVFPEDREITGILLGYRLFIP
                     GETLPPLDEAEWFPGCSGKFNHKYNLIHTWSAIAEADWSISLPMLCELQKKYGDRIFI
                     SALSSQDFDYISQFVESLPSGIQYAAMPFPLYEAYGIENEEYGITYLFDSERTLLWKG
                     NAAETGSILSCLFEKNGSKETLLKHHSDLETFKAMVSECKSRKKMKEKDLEKLLSAAE
                     TVLKINPTDFSVLASTCSQASRIGKEKVSEVCAAFDISSFNADQLLRFVKGSLISLKS
                     QHIPYPYAVKCLEQALRLSPDSAECLLTYSDVLCFMHLTDESLQMVERGLKAFPERKE
                     FYFRKKILENIIESKSAYKAN"
BASE COUNT          462 a          373 c          326 g          502 t
ORIGIN      
        1 ccttgcttcc gattttcaac ggagaatttt ctttcagagt ctttttcatg gagtgcataa
       61 taatctctgc tgaaaaaagg tcaacaatta tgcaggggac cggaaaatta cttctgttcc
      121 ggtgctgaag aaatacagct caatttgctt tataagcaga cttactttct atgatatttt
      181 caagaatctt tttcctgaag taaaactctt tgcgctcagg aaaggccttc agtcctctct
      241 caaccatctg cagtgactca tccgtcaaat gcataaagca aagaacatct gaataagtca
      301 gcagacattc tgccgaatca ggagaaagac ggagtgcctg ctccagacat ttgaccgcat
      361 aaggatacgg tatatgctga gatttcagtg aaatcagaga accctttacg aatcgcagaa
      421 gctgatctgc attgaaagaa gatatgtcga atgctgcaca gacttcagaa actttttctt
      481 ttccaatcct tgatgcctga ctgcaggttg aggccagtac actgaaatca gtcgggttga
      541 tcttcagcac agtttctgcg gcagagagaa gtttttccaa atccttctct ttcatcttct
      601 tacgggactt gcactcggaa accattgctt taaaagtttc cagatcggag tgatgcttca
      661 gaagggtttc cttacttccg tttttttcaa acaggcagga aaggattgag cctgtttcag
      721 ctgcattccc cttccagaga agagttctct ctgaatcaaa cagataagta atcccgtatt
      781 cctcattctc aataccatat gcttcatata atggaaatgg catcgccgca tactgaattc
      841 cggaaggcag gctctccaca aactggctga tataatcgaa atcctgagag gagagtgctg
      901 aaatgaaaat cctgtccccg tattttttct gcagttcaca cagcatagga agagatatag
      961 accaatccgc ctcagctatg gctgaccatg tatgaatcag attatatttg tgattaaatt
     1021 ttcctgagca tccgggaaac cattctgctt catctagagg aggaagtgtc tcgccgggaa
     1081 taaacagccg atagcccaaa agtattcctg tgatttccct gtcttccgga aaaacttcca
     1141 gcagttctgc agccagtttt ttcagcctga ttcttttttc gagatctgca gaacctttct
     1201 cttgaatttc caaatgcagt gcaagaaact cttttctttt ttctgcaggc ttttcagaac
     1261 tgtcggcttt caacaggatg ttttctttcc caaattcact catactgtgc agcgtaatct
     1321 ctgctttgaa caggtcaaca aaaatgactg cagatgtatg gagtctcagc tgtacaatca
     1381 tatccaggga tcactatgac catgatctcg atacacttca aaaaaaggtg aatttatgac
     1441 gatgcagcag tctgctgctc acttaccggt cagcctctgc ccttctgcag aagctggtct
     1501 gcatcgaaag aaggtgtgtc taataacaca gacttcagaa ttttttccaa tccttgccgg
     1561 cagctgacga tgtatgaatt atattatatt tgcttttaga aatttatttc aaatccggag
     1621 gaagcacttc gcctcttgag ttttttacag taaatttcct ttc
//
LOCUS       DAOQGJ010001022         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53624_length_1663_cov_2.414801, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001022 DAOQGJ010000000
VERSION     DAOQGJ010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53624_length_1663_cov_2.414801"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK683_14630"
     CDS             complement(<1..534)
                     /locus_tag="PK683_14630"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNH09730.1"
                     /translation="MTELTGNRRTIKSDIRLSGIGLHSGKKVNLNLFPAESGTGLVFV
                     SNQNRAKQFKIPVALDTVVDTSNAVTLGDGVNIVQTVEHLLAGLFTRGITDLVMEIDS
                     PEIPIMDGSSKPFLEAIDKSNFEEFDEAVEPIRVINPLWVMDGEKYIIILPNDTLKVT
                     YHIDFNHPLLKGQSFTTD"
     gene            complement(946..1608)
                     /locus_tag="PK683_14635"
     CDS             complement(946..1608)
                     /locus_tag="PK683_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004506326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNH09731.1"
                     /translation="MNSLPFDIIAFDIDGTIFSSEEIILDTYVLSIRKFSAENRRNVR
                     IPERNEILNEVGKPVRKIFENLLPELSLEERDSISDNVLLLLCEKINSGEGHYYPDVF
                     KTVSALSKKYRIAAASNGRLAYVSSILNYSKIMPFFLPIAVIDQKEILEKGDIVKKYS
                     SEYGVPAERILMVGDRFSDWEAARKSGAKFAYCSYGHANPGEIPDFEIELKSFSDLLD
                     FL"
BASE COUNT          480 a          360 c          313 g          510 t
ORIGIN      
        1 atcagtcgta aagctctgac ctttcagaag gggatgattg aagtctatat gatatgttac
       61 ttttaatgta tcgttcggaa gaatgatgat gtatttttct ccgtccataa cccagagagg
      121 attaatcact cgtatcggct cgactgcctc gtcgaattcc tcaaagttgg acttgtcgat
      181 tgcctcaagg aatggttttg aagaaccgtc catgattgga atttcaggag aatcaatttc
      241 cattaccaga tcagtaatgc ctcttgtaaa aagacctgcc agaagatgtt ccactgtctg
      301 aactatgttt acaccgtcac ccagagtgac tgcattgctt gtatcgacca ctgtgtcaag
      361 tgctaccgga attttgaact gttttgccct gttctggttt gatacaaaaa caagccctgt
      421 tcccgattct gcgggaaaaa ggttcagatt cactttcttg cctgaatgaa ggcctattcc
      481 tgaaagtctg atatctgact ttattgttct tctgtttcct gttaactctg tcatacattc
      541 ctcttccacc catcatgcaa gcttcatgcc aattttcgga taaaaaaagg tgaaaaatac
      601 tgaaatttgc tcattttata ctgtcaaaac ctctctcttt gcatctttcg aaagaacagg
      661 cgtggtaatt ctgcaacact gtatcatttt taatacacta aatttttgta ggggttcaaa
      721 acgagcattg atacgcagac tatcgatgga gtcctctctc atccaaagca tacgttcatt
      781 tgacgatttc agttttcgac attgacatag aaaaattcag cacaggaagg atgctttgac
      841 gctgtcagca acaaaggaat aaaatatagg tacaggaaag ggaaaacctc gctgagcaaa
      901 tgactgcaga ccccgtccct ctgtgttttc gctttctcca tcattctaaa gaaagtcaag
      961 aaggtcggag aaacttttca gttcaatttc aaagtcagga atctcaccgg gatttgcatg
     1021 accgtaggaa cagtaggcaa actttgcacc tgatttcctg gccgcttccc agtccgaaaa
     1081 tctgtctccc accatcagta ttctttcagc aggaacacca tattcggatg aatatttttt
     1141 tactatatcc cctttttcca gaatttcctt ctgatcaatg acagcaatcg gcaggaaaaa
     1201 gggcataatc tttgagtaat tcaaaatcga actcacatag gccagtcttc cgtttgatgc
     1261 agcagcaatc ctgtattttt tggaaagggc actcactgtc ttaaaaacgt caggataata
     1321 atgcccctcc cctgaattga tcttttcaca gagaagaagc aggacgttgt cagaaatgga
     1381 atctctttct tcaagtgaaa gttccggaag aaggttctca aaaatcttcc tgacaggttt
     1441 tcctacttca ttcagaatct catttctttc cggaattctg acattcctgc ggttttctgc
     1501 agaaaacttt cgaatggaca gtacataggt atccagaatg atttcttccg atgaaaagat
     1561 cgttccatca atatcgaatg cgataatgtc aaaaggtaaa ctgttcactt tgacaggaaa
     1621 tgagtcagtt ccaatgcaat tttattgatt gcataatcca tat
//
LOCUS       DAOQGJ010001023         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53744_length_1660_cov_4.581308, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001023 DAOQGJ010000000
VERSION     DAOQGJ010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53744_length_1660_cov_4.581308"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PK683_14640"
     CDS             complement(<1..851)
                     /locus_tag="PK683_14640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH09732.1"
                     /translation="MESKTNAVSMHKNLLIIFLSQALVQSGSILFTSVNALIGLEISG
                     TESLSTLPGSLMILAALILTIPASFYMARFGRRSGFLLGLAFGILGTSLSIFGIEQKN
                     LLLFCAGTVFIGFMNGFSTYFRFAAAEVSHRDFKSRAVSLVLASGVIAALVGPNLYKW
                     GLQMFENLGFMGGFILTYPLYGGAVICLLFLRTEKPAPMNWKTSRSASELFLHTPLLK
                     IIGLGSISYTVMVLIMTATPLGMKHHHFEMNDITQIIQFHILGMFVPSFFTGSLIKKF
                     GDRKVMMA"
     gene            complement(983..>1660)
                     /locus_tag="PK683_14645"
     CDS             complement(983..>1660)
                     /locus_tag="PK683_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HNH09733.1"
                     /translation="RCIAMVIVWNDLAKGDNEYVAGLVAFNSIFQVLFYSIYAYIFVT
                     YLPGIFGIKGAEVNISISQIAESVFIYLGIPFIAGMITRFTLVRAKGRDWYQNVFVPK
                     ISPVTLVFLLFTIVVMFSYKGSLIVQIPLDVIRIAVPLLFYFAIMFFSAFFLAKRAGT
                     DYSKCVSLAFTAAGNNFELGIAVAIAVFGINSGVAFAAVIGPLIEVPALILLVNFALR
                     QEKRFSV"
BASE COUNT          562 a          359 c          305 g          434 t
ORIGIN      
        1 gccatcatta cttttctgtc accgaatttt ttgatcagac ttcctgtaaa aaaagaagga
       61 acaaacattc ccagaatatg aaactgaatg atctgcgtaa tatcgttcat ttcaaaatga
      121 tgatgtttca ttccaagagg agttgctgtc ataatcagca ccatcactgt ataggaaatg
      181 cttccaagtc cgatgatctt cagaagaggg gtatgaagaa aaagctcaga tgcagatctg
      241 cttgttttcc agttcatcgg agcaggcttt tccgtgcgga ggaacagaag gcatataact
      301 gctcctccgt ataaagggta ggtaagaata aaacctccca taaatccgag attttcaaac
      361 atttgaagtc cccatttata gagattgggt cctaccaatg cagcaatgac accgcttgcc
      421 agcacaagcg atactgctct tgacttaaag tcgcggtgag aaacttctgc agcggcaaaa
      481 cgaaagtatg tggaaaatcc attcataaaa ccgataaaga cagtaccggc acagaagagt
      541 aaaagatttt tctgttctat tccaaagata cttaggcttg tccctagaat tccaaatgcg
      601 agtccaagaa gaaaaccgct tctgcggccg aatcttgcca tataaaatga tgcaggtatc
      661 gtcagaatta atgctgccaa aatcatcaat gagccaggca gtgtggaaag ggattcagtt
      721 ccgctgatct caagaccgat caatgcattg actgaagtga aaagaattga tcctgactgc
      781 acaagagcct gactgagaaa aatgatcaga agattcttat gcatagaaac tgcatttgtt
      841 tttgactcca tctatacatt ccaaatcagg catatctcag attcaatcat atttttgata
      901 agcgtactac tgcgcagtat tcaatttgca gattcaattg ctttgtcatc tgagcagact
      961 aatgagccgt tcaaaatcat catcatacag aaaatctttt ctcctgtctt aatgcaaaat
     1021 tcacaagcag gataagggca ggcacttcga tcagaggccc tattacagct gcaaatgcaa
     1081 ctcctgaatt gattccaaaa acagcaattg caacagcaat tccaagttca aaattgtttc
     1141 ctgctgcagt gaaagcaagt gatacacatt tggaataatc agtccctgct cttttcgcaa
     1201 gaaaaaaagc actgaaaaac attatggcaa aatagaacag aagcggtaca gcaatcctta
     1261 tcacatccaa aggtatctgc acgatcagac ttcctttata actgaacata actacaatcg
     1321 taaacagaag aaataccaga gtcactggac tgatcttagg aacaaaaaca ttctgatacc
     1381 agtccctgcc ttttgctctg acaagcgtaa acctggtgat cattcctgca atgaaaggga
     1441 ttcccagata aataaatacg ctttcagcaa tctgagaaat actgatattc acttccgccc
     1501 cttttattcc gaaaattccc ggcagataag tgacaaaaat ataagcgtaa atactgtaaa
     1561 acaaaacttg aaatatgctg ttgaatgcaa ccagaccggc gacatattca ttgtctcctt
     1621 ttgcaagatc attccagaca atcaccatcg caatgcatct
//
LOCUS       DAOQGJ010001024         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53758_length_1660_cov_3.113396, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001024 DAOQGJ010000000
VERSION     DAOQGJ010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53758_length_1660_cov_3.113396"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PK683_14650"
     CDS             complement(<1..673)
                     /locus_tag="PK683_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09734.1"
                     /translation="MRVRKFFPVLFIILLPFPAADLRSEDNKYFDYIYVNANTGQSSG
                     GHTAVRIGDLVYHFQFYPDEIFHFVRESWKSFLFVYGSLDSRSIFLKRVRVSEKTYSF
                     LSDELNRFYLTRTMQISLYEVLKRDLEMAELAEQADAVFPLKGAGYFQKKTGTAEGLG
                     KLRRYLNDIRSVQFGKVFLDESKIPVHTDRNAFYRLRTGYSESVIAETAFREIEKIIS
                     EDSVLK"
     gene            complement(693..1034)
                     /locus_tag="PK683_14655"
     CDS             complement(693..1034)
                     /locus_tag="PK683_14655"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH09735.1"
                     /translation="MDWAVISYGVFLVFLTVSPFLIRVRWGNSEKDSASLRNFYMEEL
                     KDLKAEFDSGKLNESEFRELASETVRKIKGVSEASVTQAESRCTQCGSLIGLPSAKFC
                     PECGTAVSRNL"
     gene            complement(1125..1577)
                     /locus_tag="PK683_14660"
                     /pseudo
     CDS             complement(1125..1577)
                     /locus_tag="PK683_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002098643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
BASE COUNT          457 a          408 c          318 g          477 t
ORIGIN      
        1 ctttcagaac ggagtcttct gaaatgatct tctctatttc ccgaaaggca gtttctgcaa
       61 taacgctttc tgaataacca gtacgaagtc tgtaaaatgc attcctgtcc gtatgcacag
      121 gaatttttga ttcatccaaa aagactttgc cgaactgtac agaacgtata tcattcagat
      181 atcttctgag ttttccgaga ccttctgctg ttccggtttt tttctgaaaa tatcctgcac
      241 ctttcagagg aaagaccgca tcggcctgtt ctgccagctc cgccatctca agatcccttt
      301 tcagcacctc atagagcgag atctgcatag tcctcgtcag ataaaaacgg ttcagttcgt
      361 ctgaaagaaa ggaataagtc ttttcagaaa cacggactct cttcagaaaa atgcttctgc
      421 tgtcaagact cccgtagaca aaaaggaaac ttttccagga ctccctgaca aaatgaaata
      481 tttcatcagg ataaaattga aaatgatata caaggtcgcc gattctgaca gcagtatgtc
      541 ccccgctcga ctgtcccgta ttggcattta catatatata gtcaaaatat ttattatctt
      601 cggatctgag atctgccgca ggaaacggaa gcagaatgat gaacaggaca ggaaaaaatt
      661 ttctgaccct catgaatgct tcccttcgaa tctcagaggt tccgtgaaac tgcagttccg
      721 cattctggac agaattttgc gctcggaagt ccaattaagc tcccgcactg tgtgcatctg
      781 ctctcagcct gagttactga agcctcagat acccctttga tcttcctgac agtctcactg
      841 gcaagttccc tgaattccga ctcattcagc tttcctgaat caaattccgc cttcagatcc
      901 ttcagttctt ccatataaaa attccgcagc gaggcggaat ccttctcaga atttccccag
      961 cgcactctga tgagaaaagg agaaactgtg agaaacacaa gaaacactcc gtatgagatt
     1021 acagcccagt ccatttattt ttcctgtttc agttctttca gtattctgtc cagatcatcc
     1081 gattctgatg cagacatttt cctgcctgca tcattttttc tgttccttct gaaatacagt
     1141 ataatgatgc ttattcccgc gagggcggct gcccctatgc tcagattgat ccagatcggc
     1201 tcaggttccg caagaatctt ttctccgaat ccgtttacaa tgcttcttgc cattccttcg
     1261 cttcccaatg acagaaattt ctgaacaata tcgtccttca gtgcttcttc gccgaacccg
     1321 gtctgcattt tacggataat ttcctctgca gactctccct gccggattct gttttcaata
     1381 aaattcttca gaagtgcaga cacttcgcag ttgttgaatg aacagctttt gatcggaaga
     1441 gaaggaagac agatgcatct gatgcgggag gtcacgatcc tgaatttttc ccggtctgaa
     1501 ggatcagtca ggttcgtgtt cacagattca gaatataatg aaattaccat gaatactgca
     1561 gcaaaaatca ttttcatgcc gttttctccc ctgcgggaac aaggatgaga agaccggaaa
     1621 gaaagaaaag aagagatccc agccatatca gcttcacaag
//
LOCUS       DAOQGJ010001025         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53850_length_1659_cov_1.814214, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001025 DAOQGJ010000000
VERSION     DAOQGJ010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53850_length_1659_cov_1.814214"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PK683_14665"
     CDS             <1..1119
                     /locus_tag="PK683_14665"
                     /inference="COORDINATES: protein motif:HMM:NF015012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HNH09736.1"
                     /translation="KKSKIFLLCFVSLYCNAWTGGYLKDYTTEPVFSSDKMETVADLN
                     YPPGNIAVSSKGRVFITFHPEGNPEIKVAELSEGKAVPFPDREFQSKRKDAPFFDTVL
                     SLRIDSKDRLWTLDFGGFGFGKPRLLAFDINTGKVVHDYIFPSEISELGSMLNDFQVS
                     PDADYIYIADASFFRRNYGIIIYDIKNRISRKVLVNHESVKPENYIAVVDGMRQTFFG
                     ILANQPGVDSIALDKKGQWLYYSAVTSEKLYRIHTSHLLNASMPAKELENKVEVFAMK
                     SMSDGLTMDLSENIYISDMEHGAVNLLSPDKKLKTLLKDPVKIRWPDGFSFGPDGWLY
                     FTCSSLQYVILKSRNSVQEKSPYQVYRFKPGYKGLPGQ"
     gene            complement(1147..1635)
                     /locus_tag="PK683_14670"
     CDS             complement(1147..1635)
                     /locus_tag="PK683_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH09737.1"
                     /translation="MGKRDKTVPADREITAAIGEYLKKVRKEKGYTIKKISEILMQKY
                     SDDVSANMLGKTERGEAKISVRLFLELCDLFMLNFYNFHFIFPHFQPIVQFDEKLKKI
                     SLDKDCRELIDFLGESQENMELAGTLKLIGSEGLKPVYEKGRAKTGKLIYLTAASKNK
                     KR"
BASE COUNT          477 a          351 c          346 g          485 t
ORIGIN      
        1 aagaaatcga agatttttct actgtgcttt gtatcattat actgcaatgc atggacagga
       61 gggtatctga aggattatac cacagaacct gttttttctt cggataagat ggaaacagtg
      121 gcggatttga attacccacc cggaaatatt gcagtctctt ccaaaggaag agtgttcatt
      181 accttccacc cggaaggaaa cccggaaata aaagtggcag aactttcaga gggaaaggct
      241 gtcccttttc cagacagaga gttccagtct aaaagaaaag atgctccttt ctttgacaca
      301 gtgctttcac ttcggatcga ttccaaagac agactctgga cactggattt cggcggattc
      361 ggattcggaa aaccgcgtct gctcgcattc gacataaata caggcaaggt tgtccatgat
      421 tatattttcc cttcggaaat ttcagaactg ggatccatgc tgaacgattt tcaggtttcc
      481 ccggatgccg attacatcta tattgcagat gccagtttct tccgacgtaa ttatggaata
      541 attatttatg atattaaaaa caggatatcc agaaaagtcc tggtcaatca tgaatcggta
      601 aaaccggaaa actatattgc tgtcgtggac ggaatgaggc agacattctt cggaattctg
      661 gcaaatcagc ctggagtgga ttcaatcgca ctggacaaaa aaggccaatg gctgtattat
      721 tctgcagtca cttcggaaaa actttacagg attcacactt cccatcttct gaatgcttct
      781 atgccggcga aagaactgga gaacaaggtg gaagtatttg caatgaaaag catgagcgac
      841 ggactgacaa tggatctgtc agagaatatc tacatttcag atatggaaca cggtgcagta
      901 aatctgctga gtccggataa aaaactgaaa accctgctga aagatcctgt gaaaatcaga
      961 tggcctgacg ggttcagctt cggaccggac ggctggcttt atttcacctg cagttcgctc
     1021 cagtatgtga tactgaaaag caggaattct gttcaggaga aatctcccta ccaggtgtac
     1081 agatttaaac caggttataa aggactgccg ggacaataag tataattaaa gtatattgaa
     1141 tagattttag cgttttttat tttttgaagc ggcggtaaga tagattaatt tccctgtctt
     1201 cgctcttcct ttttcataga caggcttcag accttccgat ccgatcagtt tcaatgtacc
     1261 tgcaagttcc atattttcct gcgattctcc cagaaagtcg atcagctccc tgcagtcctt
     1321 gtccagactg attttcttca gcttttcatc aaactgtaca atcggctgga aatgcggaaa
     1381 tatgaaatgg aaattgtaga aattgagcat aaacagatcg cagagttcca ggaaaagacg
     1441 gacactgatt ttcgcctcgc ctctctcggt cttgccaagc atatttgcag agacatcatc
     1501 tgaatatttt tgcattaata tttctgagat tttctttata gtatatcctt tctcctttct
     1561 taccttcttc agatattcac ctatggcagc agtaatttcc ctgtctgccg gaactgtctt
     1621 atcccgtttt cccatacttc ctttttacag aacggtaaa
//
LOCUS       DAOQGJ010001026         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53947_length_1657_cov_2.954432, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001026 DAOQGJ010000000
VERSION     DAOQGJ010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53947_length_1657_cov_2.954432"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PK683_14675"
     CDS             <1..760
                     /locus_tag="PK683_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH09738.1"
                     /translation="NPDGGFINPGLNTVTGYFDQTSRDLPEEMRVLDYIKKNTGEYIT
                     TEDGVKITASKMLEKFLFDDRMKYSEIKKLSGGERRRLYLVQILMNSPNFLILDEPTN
                     DLDIRTLSVLEDFLISFPGTIVIVSHDRYFLDRTAETLLIFKGSGKIESYTGTYTSFL
                     ENPNLNKKEEKIIKNEIQEETPKEPAKKRSFKEEKELKNLEEQILQLEMTKEDIEREL
                     AENSADYKRVEELSKRLQDIEKDIEKTWGRISAI"
     gene            complement(803..1447)
                     /locus_tag="PK683_14680"
     CDS             complement(803..1447)
                     /locus_tag="PK683_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09739.1"
                     /translation="MRFKTIIVSIIGFIYGCASAGEKSVDGRGLVEGRKILGYTQFQV
                     WSKEPMLGYIPNEEDPTILKDQYLKPVKVREGEGKQVIEISYSAADLGWAKAQLQKVA
                     SVNAGLEDVEAVTFRFIDPSEHVMEDYKPTSKAKKDENALKKRYIAAALKVKRIEVVL
                     KTKGGAELKTEADFRKQNILLTAGLKHDSVQNSVYTGEDRFVGYKLLSMPKDIK"
BASE COUNT          506 a          390 c          322 g          439 t
ORIGIN      
        1 caacccggac ggaggattta tcaatcccgg cctgaacaca gtgacaggat attttgatca
       61 gaccagccgg gatcttccgg aggaaatgcg ggtactcgac tatatcaaga aaaataccgg
      121 tgaatatatt acgactgaag acggagtcaa aatcactgca tccaaaatgc tcgaaaaatt
      181 tctcttcgac gacaggatga agtattctga aatcaaaaaa ctttcgggag gagaaagaag
      241 aaggctttac cttgtgcaga tactgatgaa cagtccgaat tttctgatcc tggatgaacc
      301 tacaaacgat ctcgacatcc gtactctgtc agtcctcgaa gactttctca tcagttttcc
      361 aggcacgatt gtaattgtat cccatgacag atattttctc gacagaactg ctgagacact
      421 tctgatattc aaaggcagcg gaaaaataga gagctacact ggaacatata cctcatttct
      481 ggaaaatccg aatttgaata aaaaagagga aaaaatcatt aaaaatgaga tacaggaaga
      541 aaccccgaaa gagccagcta aaaaaagaag cttcaaggaa gagaaggaac tgaaaaatct
      601 ggaagaacag attcttcagc tggaaatgac caaagaggac attgaaaggg aactcgcaga
      661 aaactctgca gactataaaa gagtggaaga actttccaaa agactgcagg atatagaaaa
      721 agacatagaa aaaacctggg gcaggatatc agcaatctga tatcctaatt tcatacccag
      781 tatctatttc ccttcaggtg aattacttaa tatccttggg catactgaga agtttgtatc
      841 cgacaaacct gtcctcgcct gtatacactg aattctgtac tgaatcgtgc ttgaggcctg
      901 cagtgagaag aatattctgt ttcctgaaat ccgcttccgt cttaagttcc gcaccgccct
      961 ttgtcttcag aaccacttca attcttttta ctttcaatgc agctgcaatg tatctctttt
     1021 ttaatgcatt ttcatccttt tttgccttgg atgtaggctt atagtcttcc attacgtgtt
     1081 cggaaggatc aatgaaccgg aatgtgacag cctccacatc ctcgagaccg gcattcactg
     1141 aagccacttt ctgaagctgc gcctttgccc agcccagatc cgccgcagaa taggaaatct
     1201 caatgacctg cttcccctcc ccttctctga ccttgacagg cttcagatac tgatccttca
     1261 gaatagtcgg atcttcctca ttcggaatat aaccgagcat cggttccttc gaccagacct
     1321 gaaactgagt ataacccaga atctttctgc cttccaccag gccgcgtccg tccacggact
     1381 tctcgcctgc gcttgcacat ccgtaaataa atccgattat acttacaatg attgttttaa
     1441 atctcatttt attctccatt ataaaaattc ctgcagtgat aagtctgtac ggtctgctta
     1501 tcggacagct cgaaatctga gacggaactc agcccaaaaa agtctgaaag aactcttcga
     1561 catccgtcct gcctcaatca gtgatactgt atattgtctt tcagtcaagg attaattgat
     1621 ctgatcctga gtttacgacc gcccgaagtc tgcctct
//
LOCUS       DAOQGJ010001027         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_53996_length_1656_cov_3.483448, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001027 DAOQGJ010000000
VERSION     DAOQGJ010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53996_length_1656_cov_3.483448"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1656
                     /locus_tag="PK683_14685"
     CDS             <1..>1656
                     /locus_tag="PK683_14685"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH09740.1"
                     /translation="TDDLGRYSISYPKTGSQICITVSSKSDGSTKVYDEKSGSELSSS
                     ATEKISITNIISEASAVKSGKKSDLYLSPFSRILSERVKALRAESGTSSDMDKIIRRA
                     GREAVIRFGLSKSFSGKSLSKALVDSDYPELNDLSFSDTSREETKKFYSMLAGFSYLA
                     NKNKKGSKVAVSDLDTVISAFASDFSDGVMDGKTGSGTAVTLGGASLGTNPITNVLQP
                     AILSFLSEGGTVGTGSTNISVSSSAAGAFQFNDSTAITYTESLQSASAAAVAQPVPLN
                     IKNNMTLEFRTVFGTADASVSSVEYSIDSGTYQTATGTSSWKFTLPTGTSSLKRDTKH
                     SLSIRAKDSAGNISPAVTFNIILGRNKDFNGDGYPDIAVGGTSGTPKITVYKGGPNYT
                     SITSSASADQTYTGSAANYFGYLMEYGDFNGDGFGDLIITEDGNGAASKGKISIYNGS
                     ASGLNASASGVINYETVNYNLGRQVTSGDFNGDGFDDIAVTAAANGLVYIFYGTAGSF
                     GTISASAASRILTGAHTTFGAGLAAGDLDGDGYSDLAVGEMGF"
BASE COUNT          483 a          376 c          384 g          413 t
ORIGIN      
        1 caacagatga tcttggaaga tattccattt cttatcccaa aacgggcagt cagatctgca
       61 tcactgtgtc ttccaaatca gacggatcca ccaaagttta tgatgaaaaa tcaggatcag
      121 aactcagttc atcggccact gaaaaaatca gcatcacgaa tattatctcg gaagcatcag
      181 cagtcaagtc aggaaaaaaa tccgaccttt atctttcacc gttttcgaga attctttcag
      241 aaagagtcaa ggcgctcaga gcagaaagcg gaacgtcctc agatatggac aaaatcattc
      301 gtcgggcagg aagagaggca gtcatcagat tcggactgtc taaatcattt tcaggaaaat
      361 cactaagcaa agctcttgtg gattcagact atccggaact gaatgatctt tccttttccg
      421 acacttcccg agaagaaact aaaaagtttt acagtatgct ggccggattt tcctatctcg
      481 caaataaaaa caagaagggt tccaaggtgg cagtttccga tctggataca gtcatttctg
      541 catttgcatc cgatttttcc gacggggtga tggacggaaa aacaggttcg ggaacagcag
      601 tcactctggg cggagcatcg ctcggcacga acccgattac aaatgtgctt cagccggcaa
      661 tcttaagttt tctctctgaa ggaggaactg taggaacagg aagcacaaat atttcagtca
      721 gctcatctgc agcaggagca tttcagttca atgacagcac tgcaatcaca tatacggaat
      781 ctcttcagtc tgcatctgca gcagctgtcg cacagcctgt tcctctgaat ataaaaaaca
      841 atatgaccct tgagttcaga accgttttcg ggactgcaga tgcctctgtc tcatcggtcg
      901 aatactccat tgacagcgga acgtatcaga ctgcgacagg aacctcttcc tggaaattca
      961 ctcttccgac aggaacatca tcattaaaaa gggatacaaa acattctctc tccatccgtg
     1021 caaaggattc tgcaggaaat atttctcctg cagtcacatt caatatcatt ctaggcagga
     1081 ataaggattt caacggggac ggttacccgg acattgcagt cggcgggact tccggaactc
     1141 cgaaaatcac ggtctacaaa ggaggaccga attatacatc aatcacttct tccgcatctg
     1201 cagatcagac atatacagga tctgcggcaa attatttcgg atatctgatg gagtacggtg
     1261 atttcaacgg ggacgggttc ggtgatctga tcatcactga agacgggaac ggcgctgcat
     1321 ccaaaggaaa gatcagtata tacaacggaa gtgcatcggg actgaatgca tccgcaagcg
     1381 gagtcattaa ttatgaaact gtcaattata atttaggaag acaggtgact tcaggagatt
     1441 tcaacgggga cggttttgac gatatcgcag ttactgcggc cgccaacgga cttgtttata
     1501 tattttacgg aactgcggga agctttggaa caatttctgc atccgctgca tccagaattt
     1561 tgacaggtgc acataccacc ttcggagccg gtctcgcagc aggtgatctg gacggtgacg
     1621 gctattcgga tctggctgtg ggtgagatgg ggtttt
//
LOCUS       DAOQGJ010001028         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54006_length_1656_cov_2.941287, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001028 DAOQGJ010000000
VERSION     DAOQGJ010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54006_length_1656_cov_2.941287"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1656)
                     /locus_tag="PK683_14690"
     CDS             complement(<1..>1656)
                     /locus_tag="PK683_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09741.1"
                     /translation="GDGGRGAVPLFSKLERTVYDHSTSASESAFVWKRVRPGGSPDAR
                     LGFLGESYLPAFPGAETVHSSGLSAKPWKNLELIFRQNGTAPQVTEIRADSEGKTETL
                     KSDSKEKCFSMKFSLNSRKASVKFLSQFQNHPYVDAMVFETEKGVNISPVSTMGIEMR
                     DLLTVDEENFKCGMKIFRPRLVIFQFGVNESQNLWQSSTRDEKYYKNWVKKTVQRYKK
                     ELPDTDILIISPMERIRKNEYGVYITMPEMMKIRKIHREIAEEDGIAFFDSLNAFGGT
                     GKSRELFDKGIIQEDRTHMTRQGSEYFADLLYDEIYREYRNYQGYLTDLETKRVQNSW
                     KENRKIVNFNSAEYYIFLISLSVLILFGKLSPSLKTLLLTSASILFYASWQIWPVGIL
                     IFSAALDYILSRKMEETDVKGRKKYLILSVCVNLGLLAFFKYTNFFMENIGNALSYAG
                     YKNFLKPIDILLPAGISFYTFQTMSYTIDVYRGIIPAEKSFLRFCFYVSFFPQLVAGP
                     IVRAKEFLPQINTFFRHFAVTEFKLAEGLFLILVGLSKKLAAD"
BASE COUNT          477 a          359 c          327 g          493 t
ORIGIN      
        1 cagtccgcag caagtttctt ggaaagaccg acaaggataa gaaagagtcc ttctgcaagt
       61 ttgaattctg tcactgcaaa atgacggaag aatgtattga tctgaggaag aaattctttt
      121 gctctcacaa tcggtccggc aacgagctga gggaaaaagg atacgtaaaa gcagaaacgg
      181 agaaaacttt tttctgcagg gatgattcct ctgtagacat caatcgtata ggacattgtc
      241 tgaaatgtat aaaaggaaat gcctgcagga agcagaatat cgatcggttt cagaaaattc
      301 ttataccctg cataggataa tgcatttccg atattttcca tgaaaaagtt tgtatacttg
      361 aaaaaggcaa gaagtcccag gttcacgcat acagaaagga tcagatattt ttttcttcct
      421 ttgacgtcgg tctcttccat tttcctggaa aggatataat ccagtgctgc agaaaaaatc
      481 agtattccta caggccagat ctgccaggag gcataaaaca ggatggaggc cgaagtcaga
      541 agcaaggtct tcagagaagg agaaagcttc ccaaaaagaa tgagaacaga aagggatatt
      601 agaaaaatat aatattctgc agaattaaaa ttgacgatct ttctgttttc cttccaggaa
      661 ttctgaaccc tttttgtttc cagatcagtg agatatccct ggtaatttct gtactcgcgg
      721 taaatctcat cataaagaag gtctgcaaaa tactcgcttc cctgtcttgt catatgagtt
      781 ctgtcttcct gaatgattcc cttgtcgaac agttctctgc ttttccctgt tcctccgaac
      841 gcattcagac tgtcaaaaaa agcaatgccg tcctcttccg caatttccct gtgaatcttc
      901 ctgatcttca tcatctcagg catagttata tatacaccgt attcattttt cctgattctt
      961 tccatcggag aaataatcag aatgtccgtg tcaggaagct cttttttata tctctgaacg
     1021 gtctttttta cccagttctt gtagtatttt tcatctcttg tagaagactg ccagaggttc
     1081 tgagattcat tgactccaaa ctgaaatatg acaagccttg gacggaatat tttcatcccg
     1141 catttgaaat tctcctcgtc cactgtaaga agatctctca tctcaattcc catcgtggaa
     1201 actggactga tatttactcc tttttcggtt tcaaaaacca ttgcatcgac ataaggatga
     1261 ttctgaaact gggacagaaa tttcacggaa gcctttctgc tgtttaatga aaatttcatg
     1321 gaaaaacatt tttcttttga atcagacttc agagtctcag ttttcccttc cgaatctgct
     1381 cgtatttcag tgacctgagg agctgtcccg ttctgacgga aaatcagttc cagattcttc
     1441 cagggtttcg cagaaagccc tgaactgtga acagtctcag ctccgggaaa tgcaggaaga
     1501 taggattctc cgagaaatcc gagtcttgca tcaggacttc ctcccggtct cacccttttc
     1561 cagacaaatg cagattcaga tgcagatgtg ctgtgatcat agacagttct ttcgagtttt
     1621 gaaaaaagag gaactgctcc ccttcccccg tctccg
//
LOCUS       DAOQGJ010001029         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54082_length_1655_cov_2.256875, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001029 DAOQGJ010000000
VERSION     DAOQGJ010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54082_length_1655_cov_2.256875"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="PK683_14695"
     CDS             complement(<1..1478)
                     /locus_tag="PK683_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH09742.1"
                     /translation="MNSEILMSLNAGNTVILEELYEKYKANPASVSADWKIFFQEIES
                     GSVPAYSNGKNGTVRSLDPLKDANASSLPEMGLMNLLNAYRRQGHLAADLDPLKILKP
                     DRTFIDQKLKGLRPEDLETEVQTGTSLGKTKLKNVIDWYERTYCHTIGCEQYYIVNEE
                     EREWLQSKMERTGNAYPLDKSVKLRLFKKLFEADYFENFLARKFVGKKRFSLEGGENL
                     IPIMDTIVEEAGGYNMEALVLGMAHRGRLNVLVNTLRKPASLIFAEFEEKAEKDLSYA
                     DVKYHLGYSNTIKTQSGKEVKLSLVFNPSHLEAADPVVTGNTRARQNLYKDKDRVKFM
                     PILIHGDAAFAGQGVVAETLNLMNLEGYTTGGTFHIVVNNQIGFTTLPDESRSTMYAT
                     DLAKGFQIPIFHINGDDPEAAYRTVRLLLEYRQKFHKDVIIDLICYRRLGHNENDEPA
                     FTQPYMYNIIKNHPTTYQLYEKQLLDSGEVTKAELESIKEEV"
     gene            complement(1534..>1655)
                     /locus_tag="PK683_14700"
     CDS             complement(1534..>1655)
                     /locus_tag="PK683_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09743.1"
                     /translation="AFEFGASAEDLARTCHAHPTLSEVLREAAMAVDSWSIHS"
BASE COUNT          383 a          379 c          355 g          538 t
ORIGIN      
        1 ttgacctctt ccttgatgga ctccagttcc gccttggtta cttcccctga atccagaagc
       61 tgtttctcat agagctggta tgtagtggga tgatttttaa tgatattata catataaggc
      121 tgagtgaatg ccggctcatc gttttcattg tgaccgagtc ttctgtagca gatcagatcg
      181 atgatcacat ccttgtggaa tttctgtctg tattccagaa gaagcctgac tgttctgtat
      241 gctgcttccg gatcatcccc gttgatgtga aaaatcggaa tctgaaaacc cttagcaaga
      301 tctgttgcat acattgtaga tctggattca tcagggagtg tagtaaaccc aatctggttg
      361 ttcactacaa tatggaatgt acctcctgta gtatatccct ccaggttcat cagattcagt
      421 gtttctgcca cgactccctg acctgcaaat gcagcatctc catgaatcag gataggcata
      481 aatttcactc tgtctttgtc tttgtacaga ttctgtcttg ctctggtatt tcctgtcaca
      541 acaggatctg ctgcttccag atgacttgga ttgaatacaa gggaaagctt cacttccttt
      601 cctgactgag ttttgatcgt gttggaatag ccgagatgat actttacatc ggcatagctc
      661 agatcttttt ccgctttttc ttcaaattcg gcgaagataa gactggctgg ctttctgaga
      721 gtattcacaa gaacattcag tcttcctctg tgagccattc caagaaccag ggcttccata
      781 ttgtagccgc cggcttcttc gacgattgta tccataatgg gaatgaggtt ttctcctcct
      841 tcgagagaga atctcttttt cccgacaaat tttctggcaa ggaagttttc gaaataatct
      901 gcctcgaaaa gttttttgaa aagtctgagt ttgacggact tgtccagagg ataggcattg
      961 cctgttcttt ccatcttaga ctgaagccac tctctttctt cctcgtttac aatatagtac
     1021 tgctcgcatc cgattgtgtg gcagtacgtt ctttcatacc agtcgatgac attcttcagc
     1081 ttggtttttc caagagacgt tcctgtctga acctctgttt cgagatcttc cgggcgaagt
     1141 cctttcagtt tctggtcgat gaatgttctg tccggtttca gtatcttcag cgggtcaaga
     1201 tctgcagcaa gatgtccctg tcttctgtag gcattcagaa ggttcatcag ccccatttcc
     1261 ggaagagagg aggcatttgc gtccttcagc gggtcgagcg acctgactgt tccgttcttg
     1321 ccgtttgaat aggccgggac cgatccggat tcgatctcct gaaagaaaat cttccagtcg
     1381 gcagaaactg atgccggatt tgctttgtac ttttcataca gttcttccag aatgactgta
     1441 ttgcctgcat taagactcat taatatttct gaattcataa gttttcccct gtaattctgt
     1501 tctgcagtgt atgcaaaatt tgataaaatt tcattatgaa tgtatcgacc agctgtccac
     1561 tgccatcgct gcttcacgaa gcacttctga tagagtaggg tgagcatggc aggttctcgc
     1621 cagatcttct gcagatgctc cgaattcaaa agcga
//
LOCUS       DAOQGJ010001030         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54110_length_1654_cov_3.559725, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001030 DAOQGJ010000000
VERSION     DAOQGJ010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54110_length_1654_cov_3.559725"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..820)
                     /locus_tag="PK683_14705"
     CDS             complement(392..820)
                     /locus_tag="PK683_14705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNH09744.1"
                     /translation="MKKILGLAAAAAVICTAAVFAQSKPVVKGGLKMEDQVVGTGKEA
                     TAGRLVSVHYTGWLADKDKNPDKKKKFDSSVDRKQPFEFTLGAGMVIKGWDQGVQGMR
                     VGGKRRLTIAPELGYGDRGAGGVIPGGATLVFDVELLDVK"
     gene            complement(834..1448)
                     /gene="leuD"
                     /locus_tag="PK683_14710"
     CDS             complement(834..1448)
                     /gene="leuD"
                     /locus_tag="PK683_14710"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNH09745.1"
                     /translation="MKAFTVLEGIAAPLDKANVDTDQIIPKQFLRKIERSGFGVHLFH
                     DWRFTDDAGKIDNPDFILNQERYKGANILLARDNFGCGSSREHAPWALEDYGFRCIIA
                     PSYADIFYNNCFKNGMLPVKLKSNEVDEIFQYVEKNKGAALKIDLAKQEVYAGGKTYS
                     FEVDPFRKDCLYNGLDDIGLTLKHEESISKFEEKHRKEFSWLYA"
     gene            complement(1445..>1654)
                     /locus_tag="PK683_14715"
     CDS             complement(1445..>1654)
                     /locus_tag="PK683_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNH09746.1"
                     /translation="FEWRNPGCSMCLAMNDDVLSPGDRCASTSNRNFEGRQGKGGRTH
                     LVSPAMAVAAAVEGRFTDIRNWRKN"
BASE COUNT          421 a          412 c          319 g          502 t
ORIGIN      
        1 gcatcattat caatattccg gaacagttct gtgaatggga aacttttact gagtctttgg
       61 aaggactgca tgactactgc agaaattttc ctcttgcaga ttacagaaga gaaaaaccgg
      121 gaagcgatcc ctggtacggg atttcttttt gactaagaag atttaccatg gataaacaca
      181 gcccgataag gtggtgaatg cttaaacacg cgagctgctc aaaacaccgc actcataaaa
      241 tgtcaggtaa ttctaatatt cttatactgc ggcaagtctt tcgaataact ccatattgca
      301 gaaaatcatt caatagactt gcttcagtct tcgtgttttt cgtgcccttc gcggtaaaat
      361 tcagttttta caaagtctca cgctaaaata attattttac atccagaagt tccacatcaa
      421 acacaagagt tgcaccgccg gggatcactc ctccagcacc gcggtctccg tatccgagtt
      481 caggagcaat tgtaagtctt ctttttccgc ctactctcat tccctgcact ccctgatccc
      541 atcctttgat gaccattccg gctccgagag taaattcgaa aggctgcttt ctgtccactg
      601 agctgtcaaa ttttttcttc ttatccggat tcttgtcttt atcagcaagc catccggtat
      661 aatgaacaga taccagtctc cctgcagtcg cttctttgcc tgtacctacc acctgatctt
      721 ccattttcag tccgcccttt actacaggct tggattgagc aaatactgca gcagtgcaga
      781 ttacggcagc ggctgctgcg agtcctaaaa ttttcttcat attgtctccc tgattacgcg
      841 tacagccagg aaaattcctt cctgtgcttt tcttcaaatt tactgatgga ttcttcatgc
      901 ttcagagtga gaccgatatc atccagaccg ttgtaaagac agtcctttct gaaaggatcc
      961 acttcgaaac tgtatgtttt tccgccggca taaacttcct gctttgcaag gtctattttc
     1021 agagcagcac ccttgttttt ttccacatac tgaaaaattt catccacttc attagacttc
     1081 agtttcacag gaagcatccc gtttttgaag cagttattgt aaaaaatatc tgcataggaa
     1141 ggagcgatga tgcatctgaa tccatagtct tcgagtgccc agggagcgtg ttctctggaa
     1201 gatccgcatc cgaaattgtc tctggccaga agaatatttg cacccttgta tctttcctga
     1261 ttcagtataa aatcaggatt gtcaattttc cctgcatcat cggtgaatct ccagtcgtga
     1321 aacagatgaa ctccgaaacc ggaacgttca attttacgga gaaactgttt tggaatgatc
     1381 tgatcagtat caacgttggc cttgtcaagc ggggcagcga ttccttctag tactgtaaat
     1441 gctttcaatt tttcctccag tttctgatat ctgtgaaacg tccttccact gcggctgcaa
     1501 ctgccattgc aggactcacc agatgagttc ttccgccctt tccctgtctt ccctcaaagt
     1561 tacggttgct tgtggaagca catctgtcac ccggggaaag cacatcgtca ttcatggcca
     1621 gacacatgga gcaccctgga tttctccatt cgaa
//
LOCUS       DAOQGJ010001031         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54212_length_1653_cov_1.913016, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001031 DAOQGJ010000000
VERSION     DAOQGJ010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54212_length_1653_cov_1.913016"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PK683_14720"
     CDS             <1..460
                     /locus_tag="PK683_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HNH09747.1"
                     /translation="DYGFSFAADDFGVKSSSHQVVLDLGVMIQEFKLDPISFKFKVKE
                     DKVKFLDNLAFIDYCKKLADNRDAVITAEAVEDIETLKFLLEHQIWQFQAFILFAKVP
                     VVQYKQEFGEWKDLPADVVYEILSHPKLLKEQKRTGHIFEVAKKAGLFTD"
     gene            504..770
                     /locus_tag="PK683_14725"
     CDS             504..770
                     /locus_tag="PK683_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09748.1"
                     /translation="MAILYYLAVLITASGKSTIKDRDFDTFALEAKLESGVSCQQFLE
                     MKDRDSYAAKVFKIQAGSRIEYKCLTPSELEDAKKEFLKKSGKN"
     gene            complement(859..>1653)
                     /locus_tag="PK683_14730"
     CDS             complement(859..>1653)
                     /locus_tag="PK683_14730"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HNH09749.1"
                     /translation="CETRMANLIADAFVWKSGTDIGFINGGAIRHDRGVDLIAKGTVF
                     SRTQLDSFMPFSNSLVGVNLNGYRLKQALEGSVNKLQTNRTALSSDDNDSDGPQHGNC
                     YGTVGDLDGSGRILLFNSRWRVEINPANTAQTITGTASDKSLKVAAEGRRVVGIYFDG
                     KTVYSNSSGDPASGWGTGKTSCTVKGIFFSSSLACSFYSTVLSDFYAGGGDENPSFNP
                     ELAEIANDGTAVNTGKTFGVDKNAILEYIINFSTYYPRISGRLVMP"
BASE COUNT          481 a          361 c          362 g          449 t
ORIGIN      
        1 ggattacgga ttcagttttg cagcagacga tttcggagtc aagagttcca gtcatcaggt
       61 tgtgctggat ctgggagtca tgatacagga attcaaactg gatccgatca gtttcaaatt
      121 caaggtgaaa gaggacaaag taaaatttct ggacaacctt gctttcatag actactgcaa
      181 aaaactggca gacaacaggg atgcagtcat tactgcagag gcagtggaag atatagaaac
      241 gttaaaattt cttctggaac atcagatctg gcagtttcag gcattcattc tttttgccaa
      301 agtccccgta gttcaataca agcaggaatt cggagaatgg aaggatctcc ctgctgatgt
      361 agtgtacgaa attctttcgc accctaaact tctgaaggaa cagaagagaa caggacatat
      421 attcgaagta gccaaaaaag caggtctgtt cacagactga tccttgccct tttctgcagc
      481 ctgacagtaa ttggaatagc ggtatggcaa tactttacta tctggcagtt cttattacgg
      541 cgtcgggaaa atcgacaatc aaagacagag attttgatac ttttgcactg gaagcaaaac
      601 tcgaatcagg ggtctcctgt cagcagtttc tggaaatgaa agacagagac agttatgctg
      661 cgaaggtctt caagattcag gcgggcagca ggatagaata taaatgtttg actccttcag
      721 aattggaaga tgcaaaaaaa gaattcttaa agaaatcagg taaaaactga tactctcaaa
      781 aaacccttca cacttgccaa aattcaaagc cgagaaattt cttgcacagc agacgattct
      841 ttactcaccc gaaaaaaatc aaggcatcac aagtctgccg cttatcctgg gataatatgt
      901 agaaaaatta atgatatatt ccagaattgc attcttgtct acaccgaatg tctttccggt
      961 gtttacagca gttccgtcat ttgcaatctc agccagttca ggattgaagg atgggttttc
     1021 atctccccct cctgcataaa aatctgatag aactgtgctg taaaaactgc aggcaagact
     1081 cgaggaaaag aagattcctt tgactgtgca ggatgttttc ccggtccccc atcctgaagc
     1141 aggatctcct gaagaattgg aatagactgt ctttccgtca aagtagatgc ctacaactct
     1201 tctcccctct gcggcaactt tgagtgattt atctgatgct gtacccgtaa ttgtctgagc
     1261 tgtatttgca ggattgattt ccactctcca tctggaattg aaaagaagta tccttccgct
     1321 tccgtcgaga tcgcctactg ttccgtagca gtttccatgc tgaggaccgt cagaatcatt
     1381 gtcgtcagag gaaagagctg tcctgtttgt ctgaagtttg tttacgcttc cttcaagtgc
     1441 ctgtttcagt ctgtacccgt tcagattgac tccgaccaga ctgttggaaa acggcataaa
     1501 ggaatccagc tgagttctgg aaaaaacagt gccttttgca atcagatcga cacccctgtc
     1561 gtgacgaatt gcacccccgt tgatgaagcc gatatccgtt ccgcttttcc agacaaatgc
     1621 atcggcgatc aggttggcca tccgggtttc aca
//
LOCUS       DAOQGJ010001032         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54303_length_1651_cov_4.035714, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001032 DAOQGJ010000000
VERSION     DAOQGJ010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54303_length_1651_cov_4.035714"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK683_14735"
     CDS             complement(<1..351)
                     /locus_tag="PK683_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09750.1"
                     /translation="MKKILFISLLSFLISGAVSAVSASAVITCKIKAKVIREIPNNTD
                     DPKLHKLEIAVIESTFDGGHSAGENCSKDRFSLETEISFRAKKRMTYKPNDIIEIYHT
                     TVYNMTEKGRNVYRK"
     gene            complement(415..957)
                     /locus_tag="PK683_14740"
     CDS             complement(415..957)
                     /locus_tag="PK683_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09751.1"
                     /translation="MIFYLIISLFIFSASNLGAETDLQSTLNLSAKDPEKAGEQGLER
                     LKKFHQETGDRSVRKKICQTLSGFKNSSISEKAYSAAESIVQYEKESDVNLVCVNSLL
                     HLSSDRNKSCRILVHELKKLAGKKDIQNEDEILLQRIIEVIGQQKFKGAYVSLMKILE
                     SGYSVQSKNSAKKVIESLPI"
     gene            complement(954..1532)
                     /locus_tag="PK683_14745"
     CDS             complement(954..1532)
                     /locus_tag="PK683_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09752.1"
                     /translation="MKSAFICREIMFRKNYFIEKKFQTRFILEFLSIVLVNAVLILGI
                     VYILGKHSYSLLPDGAAVLVQVDTSKAVILSQTKEGGIFPADDEGGTAYFPLKEISGK
                     SSRLYNAFDLYLIPVLAVSFLNLIVITVFGIFFSHRMAGPIHRIRNTLAEFNEKHSAD
                     PITLRKKDFFQDLASLINRSLELKKKENDKKT"
BASE COUNT          432 a          321 c          336 g          562 t
ORIGIN      
        1 ttttctgtat acattgcgcc ctttttcagt catattatat acggttgtat gataaatttc
       61 tatgatgtca ttgggcttgt acgtcattct ttttttggcc cggaagctga tttctgtttc
      121 gagtgagaaa cggtctttgg aacagttttc tcctgcagaa tggcccccgt caaacgttga
      181 ttcaatgact gcgatctcca gcttatgcag ctttggatca tctgtattgt tcggaatctc
      241 acggatgact tttgccttga ttttacaggt aatgacggct gatgcagaga ccgcagaaac
      301 agctcccgat atcagaaaag acagaaggga aatgaacagg atttttttca taatcattag
      361 accttactgt atgtagtttt gataaaaaaa tttccacctt ccggattctg tttttcagat
      421 tgggagtgat tcgatcactt ttttggcgga atttttgctc tgtactgagt agccggattc
      481 caggatcttc atcagtgaaa cataggctcc tttaaatttc tgctgaccga ttacttcaat
      541 gattctctga agaagaattt catcttcatt ttgaatgtct tttttgcccg ccagtttttt
      601 cagttcatgt accaggattc tgcaggattt gtttctgtct gaacttaagt gaagcagaga
      661 atttacgcag acgagattta catctgattc tttttcatac tgcacaatac tctctgctgc
      721 agaataggcc ttttctgaaa tggaagagtt tttgaatccg gagagggtct gacagatttt
      781 tttacgtact gatctgtctc cggtttcctg atgaaatttc ttcagacgtt ccagtccctg
      841 ctcccctgcc ttttccgggt cctttgcaga aaggttcaga gtggactgaa gatctgtttc
      901 tgctcccagg ttcgatgctg aaaaaataaa aagggatata attagataga aaatcatgtt
      961 tttttatcat tctccttctt tttcagttcg agagatctgt ttatcagaga tgccagatcc
     1021 tgaaaaaagt ctttttttct gagtgtgatc ggatctgcac tgtgcttttc attgaattct
     1081 gcaagtgtgt ttctgattct gtgtattgga ccagccatcc tgtgagagaa aaaaattcca
     1141 aaaacagtga ttacgatcag gttcagaaag cttacagcaa ggacagggat gagataaaga
     1201 tcaaatgcgt tgtacaaacg ggagcttttt ccggagatct ctttgagagg aaaataggct
     1261 gtcccgccct cgtcatctgc agggaaaatt cccccttcct tggtctggga aagaattact
     1321 gcctttgaag tatcaacctg aacaagaacg gcggctccgt ccggcagaag actgtaggaa
     1381 tgttttccca gtatgtaaac aattccgaga atcagaactg cattgacgag gacaatagac
     1441 aggaattcca gtatgaatct tgtctggaat tttttttcaa tgaaatagtt ttttctgaac
     1501 ataatttctc tgcagataaa tgcagatttc actctgcaga aatactcctt ttatttcaag
     1561 agatttactg actcattctt tactgtaagc tgtcttaata tgcactcaga atatttctga
     1621 atattttttc tccattctgt ctgactgatt c
//
LOCUS       DAOQGJ010001033         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54327_length_1651_cov_2.863409, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001033 DAOQGJ010000000
VERSION     DAOQGJ010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54327_length_1651_cov_2.863409"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            776..1432
                     /locus_tag="PK683_14750"
     CDS             776..1432
                     /locus_tag="PK683_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09753.1"
                     /translation="MGKKSKLRFDLEKINESLIEVQENFELINDQLSTHREEFTDVIR
                     ENMLGAYEYLNELLDDDKDIFADKGLRFMLEMNNIVLCGKDQEKRFEYAQFLLESQTR
                     FYENIRPIQKWYKKHDDSNVLKRASEIYVGIISSPQLFNEGNHRTGSLVTSWILLREG
                     LPPFVLNIQNAVAFFEPSSQIKFTNKREIGGKLKLPKYEKAFKKFLESYIDSSYLKKE
                     "
BASE COUNT          485 a          362 c          356 g          448 t
ORIGIN      
        1 atccttttct ttacagaacc ttgaaccgaa gaatggccac cttgcaaaga cgggtctgag
       61 acccgtctct acggcccctt tcccacagca aatccccgtg tcctccgtgg ttgctgagaa
      121 ccagtgcgcc cttgctcgca ggcgcaatgc cagtatgcta agagttttta accacgaaat
      181 tcactaaact cacgaaagga agaaattctt tttttcgtgt tttttgtgcc tttcgtggtt
      241 gatccttttc tttacagtcc tgtaagactg gtctgagacc cgtctgtatg ggctctctca
      301 caaaaaaaag cattccacct cagcccctct ccatgacgtc aggaataaag aggtcaatat
      361 tcgtcaggga ttgatctgcc tgtcctcaac agtgtcagta ccccggaaag gcctccatgg
      421 tctttggcat aggcttgaag gcggcgcgtg ccttttccgt agctcctgct gacctgccag
      481 tcatatgcgg ataaaaaagg gcagcagttc tgaataaatg aaacatttgc ttaagtatgc
      541 agaaaggggc agagtgtcaa gagtacttaa atgatggaaa aagcctgtat tcatttcttt
      601 ctcagaccgg cttttacgta cattttatta atctcagaat tgtatttcag cacagcattg
      661 atttcttccg gctggaatcc cagttcagag gagagatcct caaaattttc ctctggacat
      721 acagaacctc gattgaaaac tgctaatcgt ctgcacggtg ttttcggctg taattatggg
      781 aaaaaaatca aaactcagat tcgatctgga aaaaattaat gaatcactga ttgaagtgca
      841 ggaaaacttt gagctgatca acgatcagct gagcactcac agggaagaat tcaccgatgt
      901 gatccgggaa aatatgctcg gtgcctatga atatctgaat gaacttctgg acgacgacaa
      961 ggacatattc gcagacaagg gtctcaggtt catgctcgag atgaacaata ttgttctctg
     1021 cgggaaggac caggaaaaac ggttcgaata tgcccagttc ctgcttgagt ctcagaccag
     1081 attctatgaa aatatcagac ccatccagaa atggtacaaa aaacacgatg acagcaatgt
     1141 tctgaaaagg gcttctgaaa tttatgtagg gatcatcagc agtccccagc tcttcaatga
     1201 aggaaatcac aggacaggtt cacttgtcac cagctggatt cttctgcggg aaggactccc
     1261 ccctttcgtg ctgaacattc agaatgcggt cgcctttttt gagccttcgt ctcagatcaa
     1321 gttcaccaat aaaagggaaa tcggaggcaa gctgaaactt cccaagtacg agaaggcgtt
     1381 caagaaattc ctggaaagtt atatcgattc ttcgtatctg aaaaaggaat gaatttgaat
     1441 tttgatctga ttgtaataaa tttgtaacaa tataatgaga ctatacacag agaggtttag
     1501 aatgcataaa aaaataggaa tactgggagc aattgcctta accagtcttg taatcaactg
     1561 tcagaaatct gaatccaaga ctttgaatgg agcaggagca actttccctt atccgattta
     1621 ttcagcttgg tccatttctt ttggaaaatc c
//
LOCUS       DAOQGJ010001034         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54389_length_1650_cov_2.663950, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001034 DAOQGJ010000000
VERSION     DAOQGJ010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54389_length_1650_cov_2.663950"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PK683_14755"
     CDS             complement(<1..434)
                     /locus_tag="PK683_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09754.1"
                     /translation="MDRKKISKEDFESIIADKKNVQEYLETDPERGETFCFGVRTASN
                     PDYLTRAMYEMYETSLSRKLAIQAVRTLYRSVGLGSVGLNNYLKNLGIHLSPAEFLLL
                     VFKLQHQQGWGAPFELIEQNEKRIILQTSKTFESQVMKEWKMP"
     gene            complement(734..1261)
                     /locus_tag="PK683_14760"
     CDS             complement(734..1261)
                     /locus_tag="PK683_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09755.1"
                     /translation="MHCDGAAISSGVRKKNYILFLKGKPEGAVLADGKGELYGDKAIY
                     LIEPSDQFTLYRLIPPLVERIILTCRIYDKSHLICHQSLAPVEFGKKAEGVGRAVIAL
                     KKDGVAFPGLSVRIRKDGQIVANDISNANGVVSFRLLYGNYEVLIVRSENNIDIFDFS
                     FIPDLQGRQLDLELP"
BASE COUNT          458 a          390 c          339 g          463 t
ORIGIN      
        1 ggcatcttcc actccttcat cacctgggat tcgaaggtct tactcgtttg gagaatgatt
       61 ctcttttcat tctgttcaat aagctcaaac ggcgctcccc acccctgctg gtgctgaagt
      121 ttgaagacga gaaggaggaa ttcggctgga gaaaggtgta tccccaggtt ttttaggtaa
      181 ttgttgagtc ctacagaacc aagcccgaca gaacgataga gagtacgcac tgcctggatt
      241 gcaagtttcc tggaaaggct tgtttcatac atctcataca ttgcccttgt caggtaatcg
      301 ggattacttg cggtacggac gccgaaacag aatgtctctc cccgttccgg atctgtttcc
      361 agatattcct gaacattttt tttatcggcg attatggact caaaatcttc tttgctgatc
      421 tttttcctat ccatgaatct gatcttggat tgaacctatt taaacaatac aggtccggaa
      481 taggaatcgg ggcccattcc tctccctaaa atgaataaga acgattaaaa ataattaatt
      541 tggataacaa acatcaaaca ggtgaatatt cagagattca atgaaggaag agaagacggc
      601 gtgccagttt cattgcacgc tgattgaggg ggctatacga ccggattgat tcaatgaccg
      661 cgaaaccgct caactatcac ttgtcgaaaa ataatcccaa ttgtctttga aataaccgca
      721 gttgcgggag ggattacggt aattcaaggt ccaattggcg cccctggaga tcagggataa
      781 acgagaaatc aaaaatatca atgttattct ctgatctgac aatgagaacc tcataatttc
      841 cataaagaag acggaaggaa acgacaccat tcgcattgga aatatcattg gcgacgatct
      901 gaccatcctt gcggatacgc acagaaagac cgggaaaagc aactccgtct ttttttagag
      961 caatgactgc ccttccaaca ccctctgcct tttttccgaa ttcaaccggc gcaagcgatt
     1021 ggtgacagat aagatgactt ttatcataaa tcctgcaggt taaaataatt ctttcgacca
     1081 atgggggtat gagacgataa agagtaaact ggtcactcgg ctctatgagg tagattgcct
     1141 tgtcaccata cagttcccct ttcccgtccg ccaagactgc accttccggc tttcccttca
     1201 ggaaaaggat ataatttttt tttctgacgc cactacttat tgcggcacca tcacagtgca
     1261 tatcacaagc tgcctgtaaa agatcagagt atagaaagac cccatcagga gttgcctccc
     1321 ggaccagcga ttcaatgatt tcagcagtgc ccgacactgc ttctccccca agtaccgtga
     1381 atttccatag ttctatctca tatgctgatg ggtactgttt gtcttatttc ttctccttca
     1441 aaaaaaagaa ggcgacacat attaacaata acggagattg tttagtggag aacattgatc
     1501 cccctgctca ttattcccgc cgtggcattt attgcaaccc tctgttatgc ctcattactt
     1561 gacatccagg agcggagagt gcctttccgg acatggtatc cgatgctctt gataagcatt
     1621 ccattttcag ctctttttta cagccttctc
//
LOCUS       DAOQGJ010001035         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54393_length_1650_cov_2.433856, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001035 DAOQGJ010000000
VERSION     DAOQGJ010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54393_length_1650_cov_2.433856"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK683_14765"
     CDS             complement(<1..290)
                     /locus_tag="PK683_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004489753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNH09756.1"
                     /translation="MKTEDLPLSGVKVVDLSLLLPGPLCSMYLADLGAEVIKIENPRA
                     ADATRVMYKSESGIPALYLMLNRNKKAMTLNLKKEECREILFSLLQDADILL"
     gene            complement(287..>1650)
                     /locus_tag="PK683_14770"
     CDS             complement(287..>1650)
                     /locus_tag="PK683_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH09757.1"
                     /translation="RSVMIGNNPFLTDYRYIGVDAGFQSVKDFSPAEEDTLKITDEFF
                     RFWKSLPKEREPVFLFLNYNDPHKPYTPPAEFFAQVKADAGMEEKKRNYLGETAFVDF
                     HIGRILQFLKERKELDNILLIITSDHGEVMNRSHSVSVFNGVYTLYGHGQGLYEEDIH
                     TPLIIRLPGQKEKKEISYAVRSIDIYPTVLEALKIAEPFHIDGKSLFPLMTEKEKDDR
                     FYYGESRGVRGVRNYNYKFMEKTHEFHRPGPAWKGNIEKELSVLFNLREDPDENTPQK
                     NQRISEKLCSEIPSDTVTHNTYYIRVYGKGKSGKKAKISIRTENGEVRLLKKTERAVW
                     NSFGKNASAEVSDSSAVQEISFRLYPDFGYPDVRVEVNGRLAERGEIGAGVNDIYPYS
                     CSLKKEECRDMFFIRESEPSPSDSEFRVQLFARGINLKNEKENVILEKEAVDILKKQG
                     YIK"
BASE COUNT          394 a          393 c          338 g          525 t
ORIGIN      
        1 tccagaagaa tatccgcatc ctgaagaaga gaaaacagaa tctctctgca ttcttctttt
       61 ttcagattga gggtcattgc cttcttgttt ctgttgagca taaggtagag tgcaggaatt
      121 cctgattcag atttgtacat taccctggtt gcatctgcag ctctcggatt ttcaatcttg
      181 atgacctctg caccgagatc ggccagatac attgaacaga gaggtccggg aagaagcaga
      241 cttaaatcta caactttcac tccgctcaaa ggaagatctt ctgttttcat tttatatacc
      301 cctgtttttt caatatgtct accgcttctt tttccagaat gacattttcc ttttcatttt
      361 tcagattgat ccccctggca aagagctgaa ctctgaattc tgaatcggaa ggagaaggct
      421 cagattcacg tatgaagaac atatctctgc actcctcctt tttcagactg caggaatagg
      481 gataaatatc atttacgcct gcaccgattt ctcctctctc tgcgagcctt ccgttcactt
      541 ccaccctgac atccggatat ccgaaatcag gatacagccg gaaactgatc tcctgcacag
      601 cggatgaatc ggagacttct gccgatgcat tttttccgaa ggaattccag actgccctct
      661 ccgttttttt cagaagcctg acttctccgt tctcggtacg aatgctgatt tttgccttct
      721 ttccagattt tccttttcca taaactctga tatagtatgt attgtgagtg actgtatctg
      781 aggggatttc ggagcacagc ttttcggaga tgcgctgatt tttctgagga gtgttttcat
      841 caggatcttc cctcagattg aatagaactg aaagttcctt ctcaatgttc cccttccagg
      901 ccggccccgg tctgtggaat tcatgtgtct tttccatgaa tttataatta taatttctga
      961 cgcctctgac tcccctgctc tcgccgtaat aaaatctgtc gtccttttcc ttttccgtca
     1021 taagagggaa cagggatttt ccgtcgatat gaaacggttc cgcaattttc agcgcttcca
     1081 gcacagtagg atagatatca atgcttctga ctgcatagga gatttctttt ttttccttct
     1141 gtccgggcag ccggattatg agcggggtat gtatgtcttc ttcgtaaagc ccctgtccgt
     1201 gtccgtaaag agtgtagacg ccattgaaca cggaaactga atgagaacgg ttcatcacct
     1261 ctccatgatc ggaagtgatg atcagaagaa tattgtcaag ctcctttctt tctttcagga
     1321 actgcaggat tctgcctata tgaaaatcca cgaatgccgt ttcgcccaga taatttcttt
     1381 ttttttcttc cattccggcg tctgccttga cctgtgcaaa gaactctgca ggaggagtgt
     1441 aaggtttgtg gggatcatta tagttcagaa acagaaatac aggctctctt tccttcggaa
     1501 gggatttcca gaagcggaaa aactcatctg taatcttcag cgtatcttcc tctgccggtg
     1561 aaaaatcctt cactgactga aagcctgcat cgactccgat atacctgtaa tcggtcagga
     1621 aggggttgtt tcctatcatc actgacctgt
//
LOCUS       DAOQGJ010001036         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54660_length_1645_cov_4.664780, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001036 DAOQGJ010000000
VERSION     DAOQGJ010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54660_length_1645_cov_4.664780"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1255)
                     /locus_tag="PK683_14775"
     CDS             complement(173..1255)
                     /locus_tag="PK683_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH09758.1"
                     /translation="MNLSSAEIENMVKIDGSKPILVTGGTGYIASWIVKYLLDEGHTV
                     HTTVRNVQNRDRFAHLETAAKASKGRLKVFEADLLRKNSFLESMEGCELVIHTASPFF
                     VTGIKDPERELVTPALEGTRNVLDAVNASPFVRRVVLTSSCASIYGDNADIKLTKNGV
                     FTEEYWNTTSSVSHNPYSYSKKIAEEEAWKIASEQSRWDLISINPAFVLGPSLTKRTD
                     STSISTMIMLGNGTYRFGVPELWFGITDVRDVARSHILAGFSENASGRHIICSDSLEL
                     LKIAEILKKKFGSKYAFPGMKVPFFLIWLLGPLLGFSRSYIEKNIGAPVRFDNSWAKQ
                     DLGLQFTDAEKTITDHFQQLIEDGLV"
     gene            1376..>1645
                     /locus_tag="PK683_14780"
     CDS             1376..>1645
                     /locus_tag="PK683_14780"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNH09759.1"
                     /translation="MKTIEERMAGYSAYHRHPKNKLTHFFGVPMVYYSPLIALGWVQI
                     PAFGMQISLIWPVFAAVMIWYFTLDLMLASLMAVISAPIVYTCWQA"
BASE COUNT          415 a          365 c          359 g          506 t
ORIGIN      
        1 cttttcatcc ttgttctgca tatgcattct gtagctgttc atcgaatcga gaaatacttt
       61 cagagtttca gagggacttt cgagtctctg aggggtcaga ggattttccg catataatga
      121 taatgtaatg aatgaaaata taatgataaa aaacttcaat gaaattctcc tctcagacaa
      181 gtccgtcttc aatcagctgc tgaaaatgat cagtgattgt cttttctgca tcggtaaact
      241 gaagtcccag atcctgcttt gcccaggaat tgtcaaaacg gaccggtgct ccgatgtttt
      301 tttctatgta gcttctgctg aatccgagaa gcggaccgag aagccagatc aggaaaaaag
      361 gcaccttcat tcctggaaat gcatacttgg aaccgaattt ctttttgagt atctctgcaa
      421 tcttcagaag ttcgagagag tctgagcaga tgatatgtct gccggaagca ttttcactga
      481 aacctgcgag aatatgcgac cttgccacat cccgtacatc agtgattccg aaccagagtt
      541 ccggcacgcc gaatctgtaa gttccgtttc cgagcatgat cattgtgctg atgcttgtgg
      601 aatcagtccg ttttgtaaga gatggcccga gaacaaatgc aggattgatg gatatcagat
      661 cccatctgct ctgctcagat gcaattttcc aggcctcctc ttctgcgatt tttttggagt
      721 aggagtaagg attgtgagaa actgaacttg tcgtattcca gtattcttca gtgaatactc
      781 cgtttttggt gagttttatg tctgcattgt ctccgtatat cgaagcgcag ctcgaagtca
      841 ggacgactcg tctgacaaaa ggacttgcat tgactgcatc cagaacattt ctggttcctt
      901 cgagcgcagg agtgaccagt tctctttcag gatctttgat gcctgtcaca aaaaaaggag
      961 aggcagtatg aatgacaagt tcgcaccctt ccatggattc caggaaggaa ttctttcgga
     1021 gaaggtccgc ttcaaatact ttcagccggc ccttggatgc ttttgctgca gtttccagat
     1081 gagcaaatct gtccctgttc tgaacgtttc tcactgttgt gtggactgta tgtccttcat
     1141 ccagaagata cttcacaatc caggaggcaa tatagcctgt tccgccggtc actaaaatcg
     1201 gtttcgatcc gtcaattttc accatgtttt caatttctgc agaactgaga ttcattgcaa
     1261 gaggttttca gatgcagaga cttcagtcca attctctgaa cagaacgggt attctccgca
     1321 tccgtttttt aaaaaattaa ttgaccttct gcttttttcc gtttgactga aaattatgaa
     1381 aacaatcgaa gaaagaatgg cagggtattc tgcctatcac agacatccta aaaacaagct
     1441 gactcatttt ttcggagttc cgatggtgta ctattcccct ctcattgctc tcggatgggt
     1501 gcagattcct gcattcggaa tgcagatatc tctgatctgg cctgtttttg cagcagtgat
     1561 gatctggtac tttactctcg atctgatgct tgcttctctg atggcagtca tttctgcacc
     1621 gattgtctat acctgctggc aggca
//
LOCUS       DAOQGJ010001037         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54667_length_1645_cov_3.949057, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001037 DAOQGJ010000000
VERSION     DAOQGJ010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54667_length_1645_cov_3.949057"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PK683_14785"
     CDS             <1..597
                     /locus_tag="PK683_14785"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNH09760.1"
                     /translation="KAEIVPVTVGGKTPVVFDKDEFIRGKAAKDKMSGLKPAFKKDGT
                     VTAGNASGINDGACSLIVSSLDFCKSKGKKPLALIKGYGHSGCDPAKMGIGPALAIPK
                     ALKAAGISMADVGLVEVNEAFAAQYLAVQKELNLNPQITNVNGGAIAIGHPLGASGAR
                     VTLTLAYEMQRRKVKYGIASLCIGGGQGIAIILENPEA"
     gene            714..1601
                     /locus_tag="PK683_14790"
     CDS             714..1601
                     /locus_tag="PK683_14790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNH09761.1"
                     /translation="MISENKLVRFDWAIKHLLRDKANFDVLEGLLSALLKEEITILNL
                     LESESNQEVETDKFNRVDLLVEDSMKRKFIIEVQNNRETDYLERLLYGTSKAVTESVN
                     LGTGYSDIAKVISVSILYFNLGKGDDYIYYGRTDLLGIHTNHPLVVKEQVQKAENFEI
                     KVKLQTKNIFPEYYLIHTEKFEDVVNSPIDEWIYLLKNSEIKESFKSKNIDKAREKLR
                     VLNMSSEQRKHYERYLMNLASEKDIIETARQEGMTQKEDITIERALRSGKLSPEEIAE
                     MLEVSLDRVSELKRRISEH"
BASE COUNT          538 a          319 c          380 g          408 t
ORIGIN      
        1 aaggcagaga ttgttccagt aactgtcggc ggaaaaacac cggtcgtatt cgacaaagat
       61 gaattcatca gaggaaaggc agccaaagat aaaatgtccg gcctgaaacc tgcatttaaa
      121 aaagacggaa ctgtgactgc aggaaatgca tccggtatca atgacggtgc atgttcactg
      181 atcgtttctt ctctcgactt ctgcaagagc aagggaaaaa aacctctcgc tctcatcaaa
      241 ggttacggac acagcggatg tgatcctgct aaaatgggaa tcggtcctgc acttgcaatt
      301 cctaaggcac tcaaagcagc cggaatcagt atggctgacg tcggtctagt tgaagtcaac
      361 gaagccttcg ctgctcagta tcttgctgtt cagaaagagc tgaatctgaa tcctcagatc
      421 acaaatgtca acggcggtgc aattgccatc ggacaccctc tgggagcaag cggtgcaaga
      481 gtgacactca cactggctta cgaaatgcag cgcagaaaag taaaatacgg tattgcatcc
      541 ctctgcatcg gcggcggtca gggcatagca atcattctgg aaaatccgga agcgtaatgg
      601 caggctgaag ccttaaaaga agacggtgtt tcgcattgat gcagatgcta aacaccgtga
      661 gacatttgca ttatttactt gcctgcctat atatcttcct gtcttatgat tttatgatat
      721 ctgaaaataa gctggttcgg tttgattggg caatcaaaca tctactaaga gataaagcca
      781 actttgatgt tcttgaaggt ctcctgtccg cacttctgaa agaagaaatt acgatcttga
      841 accttctgga aagtgaaagt aatcaggaag tggaaaccga taaattcaac cgggtagatc
      901 ttcttgtaga agattcaatg aaacggaaat ttattataga ggtgcagaac aaccgggaga
      961 ctgattatct ggaaaggctt ctgtacggaa catcgaaagc tgtaacagaa agcgtaaatc
     1021 tcggaacggg gtactctgat atagccaaag ttatttctgt cagtatatta tattttaatc
     1081 ttgggaaagg agatgattac atatattacg gtcgaactga cctgctggga attcatacca
     1141 atcatcccct tgtcgtaaaa gaacaggtac agaaggcaga gaattttgaa attaaggtca
     1201 agctgcagac aaaaaatata tttcctgaat attatctgat tcatacagaa aaatttgagg
     1261 atgtggtcaa ctctcctatt gatgaatgga tttatctttt gaagaacagt gaaataaagg
     1321 aaagtttcaa atcgaaaaac atagacaagg caagagagaa gctccgcgta ctgaatatgt
     1381 cttccgaaca gagaaagcat tatgaaagat atttaatgaa tcttgcctca gaaaaagata
     1441 tcatagagac tgccaggcag gaaggtatga cccaaaagga agacataacc atcgaaagag
     1501 ctctccgaag tggaaaactt tcacccgagg aaattgccga aatgctggaa gtttctttgg
     1561 atcgggtttc agaattaaag agaagaattt cagagcactg aatcgggggc tccgattcat
     1621 atactgtccg gcagagttct gccca
//
LOCUS       DAOQGJ010001038         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54675_length_1645_cov_3.227044, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001038 DAOQGJ010000000
VERSION     DAOQGJ010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54675_length_1645_cov_3.227044"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..566
                     /locus_tag="PK683_14795"
     CDS             195..566
                     /locus_tag="PK683_14795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH09762.1"
                     /translation="MASNKVRSGSEKILQLKQEIKNRAGIVTVTGKIYLYEIHSLRET
                     LEDYFQRTDLKHVILELSDVDFIDSSGIGLLVANAAKFKEKGVSFCLVNLNSSVEHLF
                     RLTAIARLFKRFNSIDEALQS"
     gene            702..1463
                     /locus_tag="PK683_14800"
     CDS             702..1463
                     /locus_tag="PK683_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNH09763.1"
                     /translation="MKVVVLVKQVPDTETSIKLGDKSINEAGVKWIISPYDEFAIEEG
                     IKLREKHGGEVIAVTVGPDRTAEVLRTAYAMGVDNAVQIKVDAYNPFDTAYTSELIAN
                     FVKAENPDLVICGRQSIDSDSSQVGVQVAERLSWGHVSLALKLEVNGTSAKITREVEG
                     GTANVEASLPLVLTAQKGLNEPRYPSLKGIMSAKKKPIAVKTAADLGNPSVKVEITSL
                     EPPPARIAGRKLEAADADGFAAQLVKALREEAKVI"
     gene            1473..>1645
                     /locus_tag="PK683_14805"
     CDS             1473..>1645
                     /locus_tag="PK683_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNH09764.1"
                     /translation="MSNVLVVGEIKDGVLKKVSKELVSAGKKLADGIGGKADVLLIGD
                     GADKFAAELGAAGA"
BASE COUNT          520 a          310 c          375 g          440 t
ORIGIN      
        1 cagagcactc attgtaccta tgaaagtaaa tagaatgcag agtgagactg catagatcag
       61 aatttttgcg tatttcataa ttttatcctg taacttagcg cataaataca gcgactgaag
      121 aaaaattccc ttgctcgtct ctcctttata atatatttct tctgtgattc aagcgaaaaa
      181 tggatgaaat ttttatggcc tcaaataaag taaggtcagg ttcggagaag attttgcagc
      241 ttaagcagga aattaaaaat agagcaggta ttgtcacagt tacggggaaa atctatcttt
      301 acgagattca ttcccttcgt gaaactcttg aagactactt tcagagaact gatctgaagc
      361 atgtcattct tgaactttcc gatgtggatt ttattgattc ttccggaata gggcttctgg
      421 tggcaaatgc tgcaaaattc aaggagaaag gagtcagttt ctgtctggtg aatctgaatt
      481 ccagcgtaga acatcttttc agactgactg ccatagccag actcttcaaa agattcaatt
      541 ccatcgatga agcccttcag agctgaaaat caaatatatc aagcactgtt ttaaaaaagg
      601 cttttcttat tccccgcctg aaaattctct attcttaagg gacagatttt tttaaaagat
      661 ctctctcaaa atgtttaaaa ctaccataaa gaggcgccta tatgaaggtt gtagtgctag
      721 tcaagcaggt tccggatacg gaaacaagca ttaaactcgg agacaagtcc ataaacgagg
      781 cgggtgtaaa atggattatc tccccttatg atgaattcgc aattgaagag ggaatcaagt
      841 taagagaaaa acacggcgga gaagtcatcg cagtgactgt tggacctgac agaactgcag
      901 aagttctcag aacagcctat gcaatgggtg tagacaatgc cgtacagatt aaagtggatg
      961 catacaatcc atttgatact gcatatactt ctgaactgat agcaaatttt gtaaaagcag
     1021 agaatcctga tctggtcatc tgcggacgtc aatccatcga ttctgacagc tcacaggtcg
     1081 gagttcaggt ggcagaaaga ctttcctggg gtcacgtttc tcttgcattg aaactggaag
     1141 taaacggaac ttcagcaaaa atcactagag aagtggaagg cggaactgca aatgtagaag
     1201 caagtcttcc tctggttctc actgcacaga aaggtctgaa tgagcctaga tatccttctt
     1261 tgaaaggaat catgtctgca aagaaaaaac ctattgctgt gaagactgca gcagatctgg
     1321 gaaatccaag tgtgaaagta gagattacat ctcttgaacc gcctcctgca aggattgccg
     1381 gccgtaaact cgaagcagct gatgctgacg gatttgcagc tcagctcgta aaagctctca
     1441 gagaagaagc aaaggtgatc taaggagaat atatgtctaa cgtacttgta gttggtgaaa
     1501 tcaaagatgg tgttctgaag aaagtttcca aggagcttgt ctctgcagga aagaagcttg
     1561 ccgacggaat cggcggaaaa gcggatgttc ttctgatcgg tgacggtgcc gataaatttg
     1621 cagcagaact tggagcagca ggtgc
//
LOCUS       DAOQGJ010001039         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54694_length_1645_cov_2.386164, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001039 DAOQGJ010000000
VERSION     DAOQGJ010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54694_length_1645_cov_2.386164"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PK683_14810"
     CDS             <1..297
                     /locus_tag="PK683_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09765.1"
                     /translation="CKYWSNELRGISKMKIVILSILVFLNCGGKSYYQICRSDCSTRS
                     AICLVGFLPQQSSSASQDTTSKTALAAFLCDDYYSVCLRSCYRTTSSSSSSSTR"
     gene            315..896
                     /locus_tag="PK683_14815"
     CDS             315..896
                     /locus_tag="PK683_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09766.1"
                     /translation="MFIMIILSDKSGEEIRIQDYHRFSQENGIKVGCEEFVSSKTAAA
                     KVIKKSGYLNAEFKCVPTEKLKKFLNDAVGTDSQKMESNESALENGKKMKLEGKLLFT
                     PLKNSAKSVDAYLGKEYYIPQKDGDVILRPGRAWTSDRLKTYINKKVEVECTYYSKSP
                     EPYEQYPMTMGSEGNSVPMKRTWCEIESVKIVK"
     gene            893..1405
                     /locus_tag="PK683_14820"
     CDS             893..1405
                     /locus_tag="PK683_14820"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HNH09767.1"
                     /translation="MSSVQKWYAVYTKPKSEKKLQENLRKIDITTYLPVLAQKRKWSD
                     RVKTLEMPVFPSYLFVFLDYEKDSLKVLKDSHSVNFVTTSGTPCEIPADDIEMVKFFI
                     ESYPDKMKLDRERMFQKGRKVCIRSGPFAGKTAVIEKVKNSYYVIVNIPAVNSALTME
                     IAKENIDEFI"
     gene            complement(1436..>1645)
                     /locus_tag="PK683_14825"
     CDS             complement(1436..>1645)
                     /locus_tag="PK683_14825"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH09768.1"
                     /translation="ATDEILNEEWTFQFGGQIVSKMSKAKSIRHTVFNHMIHHRGQLT
                     MFLRLLDIKVPGIFGPTADEMPAND"
BASE COUNT          520 a          305 c          359 g          461 t
ORIGIN      
        1 tgcaaatact ggtctaatga gctgagaggg atatccaaaa tgaaaattgt aattctgtcc
       61 atactcgtct tcttaaattg cggcggcaaa agttattatc agatctgcag atcggactgt
      121 tccacaagat ctgcaatctg tctagttggg tttctgcctc agcagtcttc gtcagcgtct
      181 caggacacaa catcgaaaac tgcacttgcc gcattcctct gtgacgatta ttattctgtc
      241 tgtctcagga gctgttacag aactaccagc tccagctcaa gttccagcac gaggtaataa
      301 aaatggaaat attaatgttt attatgatta ttctttctga taaatccggt gaagaaatca
      361 ggattcagga ttatcacaga ttttctcagg agaacgggat caaggtcgga tgtgaagagt
      421 ttgtttcatc aaagaccgct gcagcaaaag tcataaaaaa atcaggctat ctgaatgcag
      481 aattcaaatg tgttccaact gagaagctga aaaaatttct gaatgatgct gtcggtactg
      541 actctcagaa aatggaatcg aatgaaagtg cattagaaaa cggtaaaaaa atgaaacttg
      601 aaggaaaact tctttttact cctttaaaaa attcggctaa gagtgtagat gcatacctgg
      661 gaaaggaata ctatattccc cagaaggacg gtgacgtaat tttgagacct ggcagggcct
      721 ggacttccga cagacttaag acatacatta acaagaaagt agaagttgaa tgcacttatt
      781 attccaaaag tcctgaacct tatgagcagt atccgatgac aatgggttct gaaggaaatt
      841 cagttccaat gaaaagaacc tggtgtgaga tagagtctgt caagattgta aaatgagttc
      901 tgtgcagaaa tggtatgctg tgtataccaa acccaaatca gaaaaaaagc ttcaggaaaa
      961 tttaaggaag atagatatta ccacttatct tcctgttctt gctcagaaga ggaaatggtc
     1021 ggacagagtc aagacattgg aaatgcctgt atttccctcc tatctgtttg tgtttctgga
     1081 ctatgaaaag gattctctga aagtgctgaa agacagtcat tcagtcaatt ttgtaacgac
     1141 atcaggaact ccctgcgaga ttccagctga tgatattgaa atggtgaagt tttttataga
     1201 atcttatcct gacaagatga agcttgatcg cgaaaggatg tttcagaaag gcaggaaagt
     1261 ctgcattcgg tcgggacctt tcgctggaaa aactgcagtg atagaaaagg tgaagaacag
     1321 ctattatgtg attgtcaaca ttcctgcagt gaattctgca ctgacaatgg aaattgcaaa
     1381 ggaaaatata gatgaattta tttagcggcc gtccaggccg ctgtcagttg aaaaatcagt
     1441 cattcgcagg catttcgtct gctgtcggcc cgaaaatccc gggaaccttg atatccagaa
     1501 gacgaaggaa cattgtcagc tgtcccctgt gatgaatcat atgattgaat acagtatgtc
     1561 tgatcgattt tgccttgctc attttagata ctatctgtcc tccgaattga aaggtccatt
     1621 cctcgttcag tatctcatca gttgc
//
LOCUS       DAOQGJ010001040         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54721_length_1644_cov_3.677785, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001040 DAOQGJ010000000
VERSION     DAOQGJ010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54721_length_1644_cov_3.677785"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="PK683_14830"
     CDS             <1..1600
                     /locus_tag="PK683_14830"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNH09769.1"
                     /translation="NTGRLNVSLSNTALFGLNKAFRFFQSNPYFCMEQRTSAYLLSIT
                     SGELLKEFSFTPPDDKSYNFTNIDGLFNGEMNDFQNEDGSFRLWKPAGSSSYPYLTAY
                     AASVMSLAKEKQHTINTAAFDKALAYLKGYIANPKEKKENALETLSLIYYVFMKEGIQ
                     DDSLEKTLLDSFDKMRFKGKAMFVLGFAHKKGLEDYRQESSLRRLYESLLSSVKFEKE
                     TVSIVSAERNRFWFSYDSESMDLAYLLRIMIRLDSSNENIYKLVQFLLAHKKGESFWM
                     DSHSSGNLALAFQEYRDQFEKNGNPEYNAKIMFHNTLLAQRLFKSDSPSNEKREFSFA
                     SLGLNGKSGKIPAVISKDSAGSRLYYSALLEYLPVKPDWKERSNGLKIDKEILKVITD
                     GKDTKYEKAGTNLKRGDTYLVKLKVTSEGPVSHLMISDPLPAFMEVINTAFATEKKSL
                     EDVKNANSGTSSEWWENSPEYTEYRDDRVLIALDYFKGGERTFSYLARPIVKGNSGSP
                     PAKAFLMYDPVKFGYSNGSSLKAE"
BASE COUNT          510 a          341 c          381 g          412 t
ORIGIN      
        1 caatactggc agactgaacg tcagtctttc caatacagca cttttcggtc tgaacaaggc
       61 gtttcgattc tttcagtcca atccttattt ctgcatggag cagaggacat ctgcatatct
      121 tctatccatt acctccggtg aacttctgaa ggagttttcc tttactcctc ctgacgacaa
      181 aagctacaat tttacaaaca tagacggtct tttcaacggg gaaatgaatg attttcagaa
      241 tgaggacgga agtttccggc tctggaagcc tgcaggaagc agcagctatc cttatctcac
      301 tgcctatgca gcaagcgtga tgtcgctcgc gaaggaaaaa cagcacacaa taaatacagc
      361 tgcattcgac aaggcgcttg cctatctgaa gggttatatc gccaatccca aggaaaaaaa
      421 ggaaaatgcg ctcgaaactc tttccctgat ctattatgtg tttatgaagg aggggattca
      481 ggacgattca ctggaaaaaa ctcttctgga ttcattcgac aaaatgagat tcaagggaaa
      541 ggcgatgttt gtgctgggat ttgctcataa gaaaggactg gaggactaca gacaggagtc
      601 ttccctcaga agactgtatg aatctctttt aagttcagtg aagtttgaga aggagacagt
      661 gagcatcgta tctgcggaaa ggaacagatt ctggttttct tatgattcag aaagcatgga
      721 cttggcatat cttctcagga tcatgatccg gctcgacagt tccaatgaga acatatacaa
      781 gcttgtgcaa tttcttctgg ctcataaaaa aggagagtcc ttctggatgg acagccacag
      841 ttcaggaaac cttgcacttg catttcagga atacagggat cagtttgaga agaacgggaa
      901 tcctgaatat aatgcaaaaa tcatgttcca taatacactt cttgcacaga ggcttttcaa
      961 atcggactct ccttccaatg aaaagaggga attcagtttt gcatctctag gcctgaatgg
     1021 aaagtccgga aagatccctg cagtcatcag caaggactca gcaggatcga gactgtacta
     1081 cagcgcgctt ctggaatacc ttcctgtaaa accggactgg aaggaaagaa gcaatggtct
     1141 gaaaattgac aaggaaatac tgaaagtcat cactgacgga aaggacacca agtatgaaaa
     1201 ggcaggaaca aatctgaaaa gaggggatac atatcttgtg aaactgaaag tcacatcgga
     1261 aggacctgta tcccatctga tgatatcaga tcctctgcct gcatttatgg aagtgatcaa
     1321 tactgcattt gctacggaaa agaaatcgct cgaagatgtg aagaatgcaa actcaggaac
     1381 ttcttccgaa tggtgggaaa attctcctga gtacactgaa tacagagatg acagagtgct
     1441 cattgcactg gactatttca agggcggaga aagaacattc tcttacctgg ccagaccgat
     1501 cgtaaaggga aactccggct ctcctcctgc aaaggctttc ctgatgtatg accctgtaaa
     1561 attcgggtat tcaaacggaa gcagtctgaa ggcggaataa gttttggatc tgagaatcgg
     1621 aacaggaatt gactttcaca ggct
//
LOCUS       DAOQGJ010001041         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54789_length_1643_cov_3.462846, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001041 DAOQGJ010000000
VERSION     DAOQGJ010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54789_length_1643_cov_3.462846"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..835)
                     /locus_tag="PK683_14835"
     CDS             complement(128..835)
                     /locus_tag="PK683_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002723527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNH09770.1"
                     /translation="MCTSLIYRNPERKLLGLGFNRDESIKRKPADPPHSYEENGIKVL
                     APADGDQGGTWIGINSDGEIYALLNLYEAQLKLIQYPTSRGFLVRRLLEKKYNTSELN
                     EKSLEKFYPFRLVRAGADRTEVFSWNGEYLENWEDSSEWLVLGSSFMLGAEAEKIRRK
                     NFEENYKNQHTDFFSAAAAFLTSHLPEKGPKSPCMHRRDAHTVSQTLILKSEKETVMK
                     YKSMQPCETEVFQEIVF"
     gene            989..1606
                     /locus_tag="PK683_14840"
     CDS             989..1606
                     /locus_tag="PK683_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09771.1"
                     /translation="MKISRIEGNLPKMKHFKIKAFIINLLILIGFNGCLTLSLPWHRS
                     SVSPELLSDSGNTEILLVYVSAEDTPRLFSEKQRTGFVYSGPRTVKALDEYLSANVSK
                     TGELEAKLRSLPLSQIEGRNTVNFSPTGTAEHSYIRSNTISVYNHYSSDFVLTAKFLN
                     RKLADRIRKEESTENENRAGVDGSLKEFKESESLKKFKKEIAINI"
BASE COUNT          469 a          339 c          337 g          498 t
ORIGIN      
        1 ttctgttgtt atgaaaacct gtaaaatacg caaagacttt tgaaaatgag tatatgctga
       61 aacagctgga caaatgcaaa tccaaaactc agatacgata tccatatatg cctgcaccgt
      121 aggtatttca gaagacgatt tcctgaaaga cttctgtctc gcacggctgc atgcttttat
      181 acttcatcac tgtctctttt tccgatttca gaatcagtgt ctgtgatact gtgtgagcat
      241 ctcttctgtg catgcaggga gatttgggac ccttttccgg gagatgcgat gtaagaaagg
      301 cggccgcagc ggaaaaaaaa tcagtatgct gattcttata gttttcttcg aaattctttc
      361 ttctgatctt ttcagcctct gcacccagca taaatgaact tcccagaacc agccattccg
      421 agctgtcttc ccagttttcg agatattctc cgttccagga aaaaacctct gtcctgtctg
      481 ctcctgctct gacaagccgg aatggataga atttttctaa tgatttttca tttaattcag
      541 atgtattata ttttttttcc aaaagacgtc tgactagaaa tcctctgctg gtgggatact
      601 gaatcagttt cagctgagct tcataaagat ttaagagtgc gtaaatttct ccgtccgagt
      661 tgatcccgat ccaggtgccg ccctggtctc cgtctgccgg tgcaagcacc tttatgccgt
      721 tttcttcgta actgtgaggc ggatctgcgg gttttctttt gattgattcg tcacggttga
      781 atccaagtcc cagcagtttt ctttctggat ttctgtaaat cagtgaagtg cacatgcggt
      841 tactctgcag tctgcagaaa atttgtaaag cccattcttg aatcttactg cctcagcggc
      901 atatgggatc tggccggaag tctcagactg cggagatata tgctgtgtct caatccctgc
      961 ttgacataag gagaatttct gaaaaaatgt gaaaatctcc cgtattgaag gaaatctgcc
     1021 taaaatgaag cattttaaaa taaaagcatt tataataaac ctattgattt taatcggttt
     1081 caatggatgt cttacattga gccttccatg gcacagaagc agtgtttcgc cggaacttct
     1141 gtccgattcc gggaatactg aaattctgct cgtttatgtt tccgctgaag atactccccg
     1201 tcttttttca gaaaaacaaa gaacgggatt tgtctattcc ggaccgagaa cagtaaaggc
     1261 actggatgag tatctttcgg caaatgtgtc taaaacagga gaacttgaag caaaactgag
     1321 atctcttcca ctgtctcaga ttgaaggcag aaataccgtg aatttttccc cgacaggcac
     1381 tgccgagcat tcgtacataa gaagtaatac aatatcagta tacaatcatt attcatcaga
     1441 cttcgtgctt actgcaaaat ttctgaatag aaagcttgcg gatcgaatca ggaaagagga
     1501 atctactgaa aatgaaaata gagcaggcgt ggacggaagt ctgaaggaat tcaaagaatc
     1561 tgaatcatta aagaaattta agaaggaaat tgcaataaat atttagttat ggctaatcca
     1621 attcttacct ggtttgaaat ccc
//
LOCUS       DAOQGJ010001042         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54794_length_1643_cov_3.272670, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001042 DAOQGJ010000000
VERSION     DAOQGJ010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54794_length_1643_cov_3.272670"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..147)
                     /locus_tag="PK683_14845"
     tRNA            complement(74..147)
                     /locus_tag="PK683_14845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Ile,seq:gat)
     gene            323..883
                     /locus_tag="PK683_14850"
     CDS             323..883
                     /locus_tag="PK683_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09772.1"
                     /translation="MNIIAAVSLFLMASFLNADSAFKKVSDKEKEKILKKINFERIKK
                     NLDVKLVLGKEKSIPYSNCSDEFPSLPGSFPCSFLSYEDVSSASEETGKSIDVQDGGF
                     INIKVSEIDSAFGGKLVIVSPGEESEFLLKIFYRKDGFISHYINKNSLIFFDWVNEKE
                     GITLKKIYLLELDEKKNASKLKRVEF"
     gene            886..>1643
                     /locus_tag="PK683_14855"
     CDS             886..>1643
                     /locus_tag="PK683_14855"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HNH09773.1"
                     /translation="MFYARMLSHTGLFPVEYSFVTVDYEVKGKKQAEGEKEFRLPLNS
                     ALGRKISLSFTGEIRCVSCGAVTKKSFNQGACYKCFITLAENDMCILRPDTCHFHLGT
                     CREPEWGQKNCFKKHTVYLANTSGLKVGITKENPVSKRWVDQGAVQALAVYETESRLE
                     AGLIESEYAKFISDKTSWQKMISGNPEKLDLRNEFQKLAEKVKLDKKLTPLSSDETEI
                     KYPVQEFPKKKVHKKFEPGVTQSDILVGIKGQYLL"
BASE COUNT          524 a          305 c          352 g          462 t
ORIGIN      
        1 aaccgatgac cctctgcttg caaagcagat gctctcccag ctgagctaaa gccccattta
       61 aatgtttctg acctgggcct gataagattt gaacttatga ccccacgctt atcaagcgtg
      121 tgctctaacc agctgagcta caggccccag taattaccat tattttttcc tctaattctc
      181 tgtaaagaac aatacggaaa aaagttccaa atactgattt ttttttgtaa ctgcattttc
      241 tatacaagaa tttggaagga agctttgcag tgccttgtca ggtatttcct gcattgtccg
      301 ataagcatat tgaggtaatt gaatgaatat tattgcagct gtttcccttt ttcttatggc
      361 gtcttttctg aatgcggact ctgcgttcaa gaaagtgtca gacaaggaaa aggaaaagat
      421 tctgaagaaa atcaattttg aaagaattaa gaaaaatctg gatgtaaaac ttgtgctggg
      481 aaaagaaaaa agcattcctt attcaaactg ttcagatgag tttcccagtc ttcccggaag
      541 ttttccctgc agttttcttt cctatgaaga tgtaagttca gcgagcgaag agaccggcaa
      601 gtccattgac gtgcaggacg gaggattcat caatataaaa gtttctgaaa ttgattctgc
      661 ctttggcgga aaacttgtaa ttgtttcccc gggtgaggag tcagagtttc ttctgaaaat
      721 tttttacaga aaagacggat tcatttcaca ttatattaat aaaaattcac tgattttttt
      781 tgactgggtc aatgaaaagg aaggaatcac tttgaagaag atttaccttc tggaactcga
      841 tgaaaagaaa aatgcatcca aactaaagag agtagagttt tgagtatgtt ctatgcaaga
      901 atgctgtcgc atacaggcct gtttcccgtc gaatattcat ttgtcacagt ggactatgag
      961 gtaaagggaa aaaaacaggc tgaaggagaa aaagaattca ggcttcctct caattctgca
     1021 ctgggcagga aaataagtct cagttttaca ggtgaaatca gatgtgtttc ctgcggagca
     1081 gtcacaaaaa aaagcttcaa tcagggtgcc tgttacaaat gttttattac gctggctgaa
     1141 aatgatatgt gcattctcag gccggatacc tgccattttc atcttggaac ctgcagggag
     1201 cctgaatggg gacagaaaaa ctgctttaaa aagcatacag tctatctggc aaatacgtct
     1261 ggtctgaaag tcggcattac aaaggaaaac ccagtttcaa aacgatgggt ggatcagggg
     1321 gccgttcagg cacttgccgt ttacgaaacg gaatctaggc ttgaggcggg tctcatcgaa
     1381 tccgagtacg caaaattcat atctgacaaa acttcctggc agaaaatgat ttctggcaat
     1441 cccgaaaaac tcgatttaag gaatgaattc caaaaattgg cagaaaaagt aaaattggat
     1501 aagaaactga cacctctttc atcagatgaa acagagataa agtatcctgt acaggaattt
     1561 cctaaaaaaa aggttcataa gaagtttgaa ccgggagtta cacagtccga tatccttgta
     1621 gggatcaagg gtcagtacct tct
//
LOCUS       DAOQGJ010001043         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54797_length_1643_cov_3.126574, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001043 DAOQGJ010000000
VERSION     DAOQGJ010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54797_length_1643_cov_3.126574"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PK683_14860"
     CDS             complement(<1..653)
                     /locus_tag="PK683_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNH09774.1"
                     /translation="MEVLPRKINSTEARQLTLLLLLKKYASGLTQKKIFSLMKNEFSN
                     ENEDSDRKKLQRDIKDLSDLGFPVRYVRQSEFSEEGIYRLLSEEKERKLEFTEDELDI
                     LSQGIIRELSVSNSAELYTAAQKIFHNKISYFPKIDLNTELKPSEENLPAVLNSILHS
                     IRNRIPLQIVYQGSFGKDAQKKFIDPIKIIKRNSRDFYLIAYDREKKEKRKYLIPKIL
                     "
     gene            complement(660..881)
                     /locus_tag="PK683_14865"
     CDS             complement(660..881)
                     /locus_tag="PK683_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HNH09775.1"
                     /translation="MQTTQPRFFKEMTVGEAIALHPEAALVFSSYHLGGCSHCSINEI
                     ETIEQVCQGYGVEVDVLLDSLNNLFEGDE"
     gene            944..1345
                     /locus_tag="PK683_14870"
     CDS             944..1345
                     /locus_tag="PK683_14870"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNH09776.1"
                     /translation="MSFSDKGSFYVRIEGRFESAHYLYDYFPDGSDEPIHGHSWKVEV
                     YLSSNSNIRPDGISFDFLTARNRLNELVHSIDHLLINDHPDFKGINPTSENIARWFYR
                     GLKTEVSKKDGKVIRIVIYEGPENIAFFEPE"
BASE COUNT          457 a          318 c          355 g          513 t
ORIGIN      
        1 aggatttttg gaatcagata ctttcttttt tctttttttt cccggtcata tgcaatcaga
       61 taaaagtctc tggaatttct cttgatgatc tttatcggat caataaattt cttctgtgcg
      121 tcctttccga agcttccctg ataaactatc tgcagaggaa ttctgttccg tatggaatga
      181 aggattgaat tcagtacagc aggaagattt tcttcactgg gtttaagctc agtattcagg
      241 tctatcttgg gaaaatagga aatcttgtta tgaaatattt tctgtgctgc agtatacagt
      301 tctgcagaat tggaaacaga aagctcccgg atgattccct gtgaaagaat atccagctca
      361 tcctccgtaa attcaagctt gcgctctttt tcttcagaca gcagtctgta aatgccttct
      421 tcgctgaatt cggactgtct gacatacctg acagggaaac ccagatcgga aagatcctta
      481 atgtctcgct gaagcttttt ccggtctgag tcttcgtttt cattggaaaa ctcatttttc
      541 atcagagaaa aaattttctt ctgagtgaga ccggaggcat attttttcag cagaagcaga
      601 agtgtaagct gtctggcttc agtgctgttg atttttctag ggagtacttc caagaaaaat
      661 tattcatctc cttcgaagag attgttcagg ctgtccagaa gaacatccac ttccacaccg
      721 tatccctgac atacctgctc aattgtttcg atctcattga tcgaacagtg agagcatccg
      781 ccgagatggt agctcgaaaa aacaagcgct gcttcagggt gaagtgctat cgcttcacct
      841 acagtcattt ccttgaaaaa tcttggttgc gttgtttgca tataatcctc ttactgccct
      901 ttatgatctg acttgaattt agtcaagcgg aaaaggagga attttgagtt tttctgacaa
      961 aggttctttt tacgtgagaa ttgaaggcag atttgagtct gctcactatc tgtacgacta
     1021 ttttccggac gggtcggatg aaccgattca cggtcattcc tggaaagtgg aggtctatct
     1081 ctcttcaaac tccaatatcc gtccggacgg gatcagtttt gattttctga ctgcaaggaa
     1141 ccggctgaac gaacttgtcc attcgataga tcatctgctg atcaatgatc atcccgattt
     1201 caaagggatt aatccgactt ctgaaaacat agccagatgg ttttacagag gtcttaagac
     1261 tgaagtctca aaaaaagacg gaaaagtgat tagaatcgtg atttatgagg ggcctgaaaa
     1321 tattgctttt tttgagcctg agtgattcag tgatctttga gggcagattc tgaagggcag
     1381 aatatgtgat tgcaaaaaat gctccatgaa atgctatgta tccatagaaa atggtatttt
     1441 aaggattgcc cttgaagccg tcggcgtctc tatgccgaat gcatcacctg acaggtgaag
     1501 tgatatgagg aattatgcag atcaaaaaag atgaaataga acttaatggc ggagaagagt
     1561 tttcaaaatt tgtaaacttc tatgatttgg ccggagcgat ccagctgaag gaacccatta
     1621 tagatcccaa tggaaatgtt ctt
//
LOCUS       DAOQGJ010001044         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54798_length_1643_cov_3.113350, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001044 DAOQGJ010000000
VERSION     DAOQGJ010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54798_length_1643_cov_3.113350"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1466)
                     /locus_tag="PK683_14875"
     CDS             complement(45..1466)
                     /locus_tag="PK683_14875"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNH09777.1"
                     /translation="MSLLLLLISDCSSEKIQQVSPKAAKGIIDLRPIHFDDEYESSIP
                     LDGEWGFHWNEFLNPESKEIFSGTFIRVPSQWQQEKNPEGISYPPYGFASYSLKVLLP
                     ENHPPLSFSMSDTGMAYKFYVNGKLLSGNGKTGRTKEEMEIRQRYTVFPVPDSSTELD
                     IVIHNSNFHYYKAGLWQSAVIGPSKIIEYRHLQNIALDLIVASSLLIMGLYHIGLFVN
                     RQKDRSPLYFGMFCILVVLRTITIKERLIFDLFPNLPFTIVHKIEYFSFYFGSLVFMQ
                     FIHSMFKEEWNEKLYKGFTYFFLGASVLVLLSPMNTYTRSLIFVQGAILAGIIYTLKI
                     LFGALKNRRFGAASFTLGLSIFFIAIIHDILRTRGYVYTPFMASYGLLFFVVSQAIVL
                     SRRFANAFVLAEKLSDELQNLTDELEMKVRQRTKELNDVLHTIQNDLAYAKRIQNDSL
                     FIDYSQFHNLEIFPYYSALFWIF"
BASE COUNT          512 a          319 c          313 g          499 t
ORIGIN      
        1 ccttcttcga ttagaatcga ttatagaatc aattagaaaa tattttaaaa aatccaaaac
       61 agcgcggaat agtaggggaa tatttccaga ttgtgaaact gggaataatc tatgaacagg
      121 gaatcattct gaattctttt tgcataagca agatcattct gaatcgtgtg cagaacatca
      181 ttcagttctt ttgttctctg acggactttc atttccagtt catctgtaag attctgaagc
      241 tcatcactga gtttctcagc cagaacaaat gcatttgcaa accttctgga caggactatg
      301 gcctgcgaca ctacaaagaa cagcagtcca taactcgcca taaaaggagt gtatacataa
      361 cctctcgttc tcagaatatc atgaatgatt gcaatgaaga aaatagaaag tccgagagta
      421 aatgatgctg cgccgaatct tctgtttttt agggcaccaa aaagaatttt aagagtatat
      481 ataatcccgg caagaattgc accctgtaca aatatcagtg atctggtata agtgttcatt
      541 gggctgagga ggaccaatac agatgcacct aaaaagaaat aagtaaaccc cttgtacagt
      601 ttctcgttcc attcttcctt gaacatactg tggatgaact gcataaaaac cagggagcca
      661 aaataaaaag aaaagtattc tatcttgtgc acaattgtaa aaggaagatt cgggaacaga
      721 tcaaaaatca gtctttcttt gattgttatt gttcttaaaa caaccaggat acagaacatc
      781 ccaaagtaaa ggggggatct gtccttttgt ctgtttacga aaagtccaat atgatataat
      841 cccattatca gaagcgaact cgctacaatc agatcgagtg caatattctg cagatgcctg
      901 tattcaatga tttttgaggg accaatgact gcactttgcc agagtcccgc cttgtaataa
      961 tgaaagtttg aattatgaat tacaatatca agttcagttg agctgtccgg aacgggaaat
     1021 actgtgtatc tctgtctgat ttccatctct tcttttgttc tgcctgtttt cccgtttccg
     1081 gaaagcagtt ttccatttac ataaaatttg taagccatac cagtatcact catggaaaaa
     1141 gagagtgggg gatgattttc cggaagaagc actttcagtg aataggaagc aaatccgtag
     1201 ggaggatagc tgattccttc cgggtttttt tcctgctgcc attgtgatgg gacacggatg
     1261 aaagttcctg aaaagatttc ttttgattcc ggattcaaaa attcattcca gtgaaacccc
     1321 cattccccat ccaagggaat ggatgattca tattcatcat caaaatgaat tggacgcagg
     1381 tctattatcc cttttgccgc ctttggagaa acctgttgaa ttttttctga gctgcagtct
     1441 gaaatcagca ggagcaggag actcaaaaca gttatcctta ctgataaaaa cattctgttc
     1501 tctctgttgc agtcagcatt tctaatttcc gtaaacttta ataattctga atgctttttt
     1561 attacaattc ttatttttat tctgaactgg aaaaagcgat cgtcagcgaa aatactgtcc
     1621 gttgcagatc cccttaagct ctt
//
LOCUS       DAOQGJ010001045         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_54899_length_1641_cov_3.383354, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001045 DAOQGJ010000000
VERSION     DAOQGJ010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54899_length_1641_cov_3.383354"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1322
                     /locus_tag="PK683_14880"
     CDS             96..1322
                     /locus_tag="PK683_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09778.1"
                     /translation="MNLNFYKKNFGKIKISLTVMIFSAVGCTKKANDNSDKIPWLLAA
                     ASLQKSASTSTSTASTYSVGGTITGLSSAGLVLQNNSGDDLTVASGATSFTFATKVSG
                     AYSVTVKTQATARNCTLTNSSGTASANVTSVAITCSSDGSSWTSKTLPSASTWTSVAY
                     GNGIFVAVASGGTAAASSPDGVTWTARTLPSSASWNSVTYGNGIFVAVAGGPSTTAAS
                     STDGITWTARTLPSSSQWNQAGYGNGVFVTAATGPSTNGASSTDGITWTARTMPASAG
                     WMSPAYGNGIWTYICSSSTTAASSTDGANWTARTMSASATWQSVAFMETEFLRQLHTV
                     ELQLRLQLTGLPGQQGHCPAHQTGDLLLLETGYFLQSLTQVQLLQLRLTELLGQQELF
                     RQVQTGLRLRMETELL"
     gene            complement(1440..>1641)
                     /locus_tag="PK683_14885"
     CDS             complement(1440..>1641)
                     /locus_tag="PK683_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09779.1"
                     /translation="YTEALEKLIEVGVQIKTDDESLIRSIGALYIAVKGQWKLAEIAY
                     EMHDDTLALWEDYNEKVANGEI"
BASE COUNT          435 a          383 c          390 g          433 t
ORIGIN      
        1 gtcttaaaca tgttctgtgt ctaatctgca taaaaactgc atggctctgt cagtcgatcg
       61 tcggtcatct gacaggttct gcaaaaggaa tttaaatgaa tttaaatttt tataaaaaga
      121 actttggaaa aataaaaata tcattgactg taatgatatt ttcggcagtg ggctgtacaa
      181 agaaggcaaa cgacaacagc gataagatcc cttggcttct tgcagctgca tccttgcaga
      241 aatctgcctc aacaagcaca tcaactgcat cgacttactc tgtcggcgga acaattaccg
      301 gcctttcttc tgcaggtctg gtgcttcaga ataattcagg tgatgacttg actgttgcca
      361 gcggtgcgac cagtttcaca tttgcgacga aggtgtcggg agcctattct gtaactgtga
      421 aaactcaggc gacagcgaga aactgtacat tgactaactc gagcgggact gcatctgcca
      481 atgtgacttc agtcgcaatt acctgctcct ctgacgggtc atcctggact tcgaaaacac
      541 ttcccagtgc atccacatgg acatcagtgg cgtatggaaa cggaattttc gttgctgtag
      601 cttccggagg aacggcagct gcgagttccc ctgatggagt cacttggaca gccagaactc
      661 ttccgtcttc agcatcttgg aattctgtga catatggaaa cggaattttc gttgcagttg
      721 cagggggccc aagcacaaca gctgcatctt caacggatgg aattacctgg acagccagaa
      781 cacttcccag cagttcacag tggaatcagg caggatacgg aaacggtgtt tttgtaactg
      841 cagcgactgg accaagtaca aacggagcca gttcgacaga cggaattacc tggactgcca
      901 gaacaatgcc agccagtgcg ggatggatgt ctccggctta cggaaacgga atctggacat
      961 atatctgcag ttccagtaca acagctgcat cctctactga cggagccaat tggacagcca
     1021 gaacgatgtc tgcttctgct acttggcagt ctgtggcttt tatggaaacg gagtttttgc
     1081 ggcagttgca tacggtggaa ctgcagctca gacttcaact gacgggatta cctggacagc
     1141 aaggacattg cccagctcat caaactggag atctgttact tttggaaacg ggatatttct
     1201 tgcagtcgct gacacaggta caattgctgc aacttcggct gacggaatta cttggacagc
     1261 aagaactctt ccggcaagtg caaactggtt tgcgactgcg tatggaaacg gaacttttgt
     1321 gacaattgct cagggaagca caagcgctgc tgtatctcag tgatttgaat tttgcgaggg
     1381 ttttatgccc tcgcagtcaa taaagtttta acatctgaaa aactcttctc agtgcttttt
     1441 cagatttctc cgtttgccac tttctcgttg taatcttccc agagggcaag agtgtcgtca
     1501 tgcatctcgt aagcaatctc ggcgagtttc cactgacctt tgacagcaat gtaaagtgca
     1561 ccgatagaac ggatcaatga ttcatcatca gtctttatct gaaccccgac ttcaatgagt
     1621 ttctcaagag cctcagtata c
//
LOCUS       DAOQGJ010001046         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55037_length_1639_cov_3.636995, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001046 DAOQGJ010000000
VERSION     DAOQGJ010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55037_length_1639_cov_3.636995"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PK683_14890"
     CDS             complement(<1..315)
                     /locus_tag="PK683_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH09780.1"
                     /translation="MKYTRLILLLGFFSGACLTPDYNRSSYRENAADVVYRGDLFALQ
                     NSIQRGASINERDSFQRKYTPLMVAAREGELEIARWLIERKADVNAVTRDGHTALMYA
                     CYN"
     gene            570..>1639
                     /locus_tag="PK683_14895"
     CDS             570..>1639
                     /locus_tag="PK683_14895"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HNH09781.1"
                     /translation="MRVKFVAYELCFIIPFFSGIMFCISKKDDSTKKLLIGSTALNTS
                     RSSSVTDTATVTTALSSSAPSALSFSGSPFSFLQGAAITTITPTVAGTITSCTSSPSL
                     PSGLIINSTTCAISGMPTTAQASANYTISAGNSAGNKTASIGILITSNGSSWTARTLP
                     SSSGWTSVTYGNGIFVTVSTSTAAASSTDGITWTARTLPSSSNWSSVTYGNGVFVTLA
                     NSSSTAAASSTDGITWTARTLPSSSNWSSVAYGNGVFVAVSAGSTAAASSTDGITWTA
                     RTLPSSQPWTSVTYGNGVFVAISDGSSAAASSTDGITWVARTMPSSSNWTSVTYGNGV
                     FAAVSASGSTAASSADGINWTA"
BASE COUNT          440 a          385 c          356 g          458 t
ORIGIN      
        1 attgtagcag gcatacatca gcgcggtatg accgtctctg gtgactgcat ttacatcggc
       61 ctttctttca atcagccatc ttgcgatttc cagttccccc tctctggccg ctaccatcaa
      121 aggagtatat tttctctgaa aggaatctct ttcattgata ctggctcctc tctgaatgga
      181 gttctgcagg gcaaaaagat cacctctgta aaccacatcc gctgcatttt ctctgtagga
      241 actcctattg tagtctggag taaggcaggc acctgagaag aatcccagaa gaaggatcag
      301 tcttgtgtat ttcatacgta tctccttttt tggctttctt tctgcagaaa gcttataacc
      361 agagtatagg ccggaaaaaa gaagctgtct ataggtaaac gaagcattta gggagattat
      421 gtacgttcat agtgggattt acccaagccg gaaaaagtca agagatatct acaagaagtc
      481 aaaaaactta tttgacaaat tcatggttat gaataattgt acgaattagt ttttaggaca
      541 aattcaaata atagcaaaag gaagcttaga tgagagtcaa attcgtggct tatgagttat
      601 gttttatcat accttttttc tcgggaataa tgttttgcat ttccaaaaag gatgatagca
      661 caaagaagct tctgatcggt tcaaccgcat taaatacgag caggagcagt tctgtgacag
      721 atacagccac agttacaacg gcactttctt cgtctgctcc atctgcactt agttttagcg
      781 gcagtccatt cagttttctt cagggggctg ctatcacaac catcactcct acagtggcag
      841 gaaccataac cagttgtact tccagcccaa gccttccctc tggactcatt ataaacagta
      901 cgacttgcgc aatcagtgga atgcccacaa cggctcaggc ttcagcaaat tatactattt
      961 ctgccggtaa ttctgctgga aataaaactg cttccatcgg cattttaatt acatccaatg
     1021 gatcctcctg gacagcgcga accctgccaa gttcttccgg ttggacttca gttacctacg
     1081 gaaacggtat ttttgttacg gtaagtacgt ccacagcagc cgccagttca acagacggga
     1141 ttacctggac agcgcgaacc ctgccaagtt cttccaattg gtcttcagtt acctacggga
     1201 atggtgtttt tgttactcta gctaacagta gttcaacagc agccgccagt tcaacagacg
     1261 ggattacttg gacagcgcga accctgccaa gttcttccaa ttggtcttca gtcgcctacg
     1321 ggaatggtgt ctttgttgcc gtatcagcgg gcagcacagc tgctgccagt tcaacagacg
     1381 gaatcacttg gacagcgcga accctgccaa gttctcaacc atggacttct gtcacttacg
     1441 ggaatggtgt ctttgttgct atttctgatg gatcatcagc cgctgcaagt tcaacagatg
     1501 gaatcacttg ggtagcaaga acaatgccaa gttcttccaa ttggacttct gtcacttacg
     1561 ggaatggtgt ctttgcagct gtatcagcga gcggatcaac agccgccagt tcagctgatg
     1621 gaattaactg gacagcaag
//
LOCUS       DAOQGJ010001047         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55043_length_1639_cov_3.389520, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001047 DAOQGJ010000000
VERSION     DAOQGJ010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55043_length_1639_cov_3.389520"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..909)
                     /locus_tag="PK683_14900"
     CDS             complement(160..909)
                     /locus_tag="PK683_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNH09782.1"
                     /translation="MQKEKIRLDELLVRRNFAPDLKRAASLILSGSVLISDSPVTKPG
                     FRFKEDADIRIREKIKNHSSRGYLKLKPVMEKFAISVRGRVCIDIGASTGGFTEILLE
                     GNAERVYALDVGYGQLAEKLRKDSRVTVMDRFHIRELNWTHIQGNKDHFFVVIDLSFI
                     SVLKAFEVLFRLKKEKPSSEFYVLALVKPQFETDPENLKNGILYNDVKRIFTLISVIR
                     RIRKDAEYLGAERAGIKGTSGNQEYFVYFKI"
     gene            complement(890..>1639)
                     /locus_tag="PK683_14905"
     CDS             complement(890..>1639)
                     /locus_tag="PK683_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH09783.1"
                     /translation="PVLCISAVLGRKNSELNRNVFLASSALECIHTYSLIHDDLPAMD
                     NDDFRRGLPTNHKKFGEAAAILAGDALNSAAFYLISLIQSQESDLVRDMIQILHSGAG
                     GDGMISGQMEDIESENNPEKFQKDTLHRIHEKKTGALILSSVLLGNRLNSDYISYEKR
                     FKAYGEKLGVLFQITDDILDEESSLEEIGKTPGKDSAKGKLTYPAFYGMDQSKKLKNE
                     IREELEIISRELPDEKNFFKELPAYIAERKN"
BASE COUNT          417 a          376 c          309 g          537 t
ORIGIN      
        1 aaagcgagct gagttaatcc taccggaggt ttcaaggcac tgtcgtccct tgaaaatccc
       61 cgtttaattc tgtgtatgac agtcttttct gataatttcc tgattctttc aacagcacag
      121 tctctgtcgg ggttcttctg aatattattt ttcattattt catatcttaa aatatacaaa
      181 atactcctgg ttgccggaag tacctttaat accggccctt tctgctccca gatactccgc
      241 atctttcctg atcctgcgga ttacagagat cagagtgaat attctcttta cgtcattata
      301 cagtatcccg tttttcagat tttccggatc agtttcgaac tgaggcttca ccagtgcaag
      361 tacataaaac tcggaagaag gtttctcttt tttcagtctg aacagaactt caaatgcctt
      421 aagcactgaa ataaaactca ggtcaataac cacaaaaaaa tgatccttgt ttccctgtat
      481 atgagtccag ttcagttccc tgatatgaaa tcggtccatc actgtcacac ggctgtcctt
      541 tcggagtttt tctgccagct gaccataccc cacatccaat gcatagactc tctctgcatt
      601 tccttcgaga agtatctcgg taaatcctcc tgtggatgca cctatgtcga tgcagactct
      661 tccccgtacg ctgattgcga atttttccat tacaggcttc agcttgagat aacctctgct
      721 ggaatggttt ttgattttct ctctgattct gatgtctgcg tcttccttga accgaaaacc
      781 cggcttggtg acaggtgaat cactgattag aacagatcca gagagaataa gtgacgctgc
      841 tcttttcaga tcaggagcga agtttcgtct gaccagaagt tcatcgagtc taattttttc
      901 tttctgcaat gtaggccggg agttccttaa agaaattctt ttcatcagga agttctctgg
      961 aaatgatttc gagttcttct ctgatttcat ttttcagttt cttggactga tccattccgt
     1021 aaaatgcagg ataagtcagt ttgccttttg cactgtcctt tccaggagtc tttcctattt
     1081 cttccagact gctctcttca tccagtatat catctgtgat ctgaaaaaga actcccagtt
     1141 tttcaccgta agctttgaat cttttctcat aagatatgta gtcggaattc agtctgtttc
     1201 ccagaagaac agacgaaaga atcaatgcac ctgttttttt ttcgtgtatt ctgtgaagag
     1261 tatccttctg aaacttttcg ggattgtttt cagattcaat gtcttccatc tgaccggata
     1321 tcattccgtc ccctcctgca cccgaatgaa ggatctgtat catatctctg actaaatctg
     1381 attcctgact ctgaatgagt gagatcagat aaaatgcagc agagttgagt gcatcacctg
     1441 cgaggatggc cgccgcttcc ccgaactttt tatgatttgt aggaaggcct cttctgaaat
     1501 catcgttatc cattgcagga agatcatcat gaatcagaga atatgtatga atacattcca
     1561 gtgccgaaga agccaaaaat acattcctgt tcagttcgga attttttctt ccgaggacag
     1621 cagaaatgca gagcactgg
//
LOCUS       DAOQGJ010001048         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55201_length_1636_cov_4.267552, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001048 DAOQGJ010000000
VERSION     DAOQGJ010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55201_length_1636_cov_4.267552"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PK683_14910"
     CDS             <1..187
                     /locus_tag="PK683_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNH09784.1"
                     /translation="MTGLHGIHVIVGACLIVWVAIKTSKGHFNHEYYTPVEGVGIFWH
                     IVDLIWIYLFPLLYLVG"
     gene            189..494
                     /locus_tag="PK683_14915"
     CDS             189..494
                     /locus_tag="PK683_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09785.1"
                     /translation="MELFINYSLYLIAAVCILVPLVGFGIAPPVIVKAALGIFAGNAY
                     SQNLQQGLLRIFIKDNADNRLLKPILPAVESLQTAMEELDNPALRHQRLKAEKGMVG"
     gene            496..1101
                     /locus_tag="PK683_14920"
     CDS             496..1101
                     /locus_tag="PK683_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNH09786.1"
                     /translation="MELFINYALYFIAGVCILVPLCGFGLAPPVIVKAALGIFIGNAY
                     AQNLQQGLLRIFIKDNANSKLLKPILPAVESLQTAMEELDNPALRHQRLKEEKKDEHH
                     GSGHHVIPIPVYVGVLLVLLVGTIITVWVAQYDFGAMNTVIAMLVATIKASFVLGYFM
                     HLKYDNMMNRVIFGSAFFFLMLLVFFSVADIWTRVHITKGF"
     gene            1101..1292
                     /locus_tag="PK683_14925"
     CDS             1101..1292
                     /locus_tag="PK683_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09787.1"
                     /translation="MSACKCGTTRQSPDAEEIAKYSKTAIFLILFGISAKPISVDFKC
                     RKCGKIIDSLSSEELKVYR"
     gene            complement(1308..>1636)
                     /locus_tag="PK683_14930"
     CDS             complement(1308..>1636)
                     /locus_tag="PK683_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09788.1"
                     /translation="YVQFMIEENGLLMNWPILKKVPKRSAKVAELACRELIRKDYNRR
                     NVSEIDRTAVLALKEKEFMLDSEDLYANPGKEAEEIVALAAHLFINVFDYQKLDKIEI
                     ELVLEQ"
BASE COUNT          437 a          326 c          343 g          530 t
ORIGIN      
        1 aatgacaggg ctgcacggta ttcacgtcat tgtaggtgcg tgtctgattg tttgggttgc
       61 aatcaaaact tctaagggcc actttaacca cgaatattat actcctgtag agggagtcgg
      121 aatattctgg cacattgtgg atttgatctg gatttatctt ttcccacttt tatacttggt
      181 aggctgatat ggaactgttt ataaactact cactttattt gattgcagct gtttgcattc
      241 tggtacctct agtcggattt ggaattgcac ctcctgtcat agtcaaggcg gcattaggaa
      301 tttttgcagg aaatgcatat tcccagaatc tccagcaggg tcttctcaga attttcatca
      361 aggacaatgc agacaacaga cttctgaagc ctattcttcc tgctgttgag tcacttcaga
      421 cagctatgga ggagctggat aatcctgctc tccgtcacca gagactgaag gctgagaaag
      481 gaatggtagg ctgatatgga actgtttatc aactatgcac tttatttcat tgccggggta
      541 tgtatcttag tccctctttg cggatttggt cttgcacctc cggttatcgt aaaggctgct
      601 cttgggattt tcatcggcaa tgcgtatgca cagaatcttc agcagggtct tttaagaatt
      661 ttcataaagg acaatgcgaa cagcaaactt ctgaagccga ttcttcctgc tgttgagtct
      721 ctgcagactg caatggaaga actggataat cctgctcttc gtcatcagag acttaaagaa
      781 gaaaagaagg acgagcatca cggatcagga catcatgtaa tcccaatacc agtgtatgta
      841 ggagttcttc tggttcttct ggttggaaca attatcactg tctgggttgc tcagtacgat
      901 ttcggtgcaa tgaatacagt cattgcaatg cttgtagcaa ccattaaggc aagtttcgtt
      961 ttgggatact ttatgcacct gaagtacgac aatatgatga acagagtcat tttcggatca
     1021 gcatttttct tcctgatgct tcttgtgttt ttcagtgtcg ctgacatttg gacaagagtg
     1081 cacattacaa aaggatttta atgtctgcct gtaaatgcgg aacaactagg cagtctcctg
     1141 atgcagagga gattgccaaa tattcaaaaa ctgcaatatt cctgatttta tttggaatat
     1201 cagcaaaacc catctcagtg gatttcaagt gcaggaaatg cggtaaaatt atagattcac
     1261 tttccagtga ggaattgaaa gtttacaggt agtcgataac gattgcctta ctgttccaga
     1321 accagttcaa tttcaatttt atccaatttc tgataatcaa atacattaat gaataaatgt
     1381 gcggcaagag caacaatttc ttctgcttct tttcctggat ttgcatacag atcttctgaa
     1441 tccagcataa attccttttc tttcagtgca agaactgcag ttctgtctat ttccgaaaca
     1501 tttcttctgt tatagtcttt tcggatgagc tctctgcagg caagctctgc aacttttgca
     1561 cttcgttttg ggactttttt caggataggc cagttcatca ggagtccatt ttcttcaatc
     1621 ataaactgaa catatt
//
LOCUS       DAOQGJ010001049         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55219_length_1636_cov_3.213789, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001049 DAOQGJ010000000
VERSION     DAOQGJ010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55219_length_1636_cov_3.213789"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PK683_14935"
     CDS             <1..529
                     /locus_tag="PK683_14935"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH09789.1"
                     /translation="RASIKNVLARYKMIQIPKKSAELEQEILETLAARIKNRHVLIED
                     VNIDETKLDPGIMNAIAEKLKREQELETMKYNQKIAEKEDEIAKMKAKRDAEVEIIAA
                     EAKAKTQRLTNVHITPAYLKLKTVEASMKALESPNAKLIFVPVGKDGLPLNVNISDKI
                     FSESGFPALPAEKKH"
     gene            complement(533..>1636)
                     /locus_tag="PK683_14940"
     CDS             complement(533..>1636)
                     /locus_tag="PK683_14940"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH09790.1"
                     /translation="VHISESEIQDAAFQASLRVMTDAIEEKKTQIRRFSEYRYGKEKE
                     FIIVIASPLMIQNSIVGVFYMDSSYLSSSLDAIDIDTVEIFSSQIALAIESGVLYEQL
                     ETEFKNKTKELNYTNERLNSIIREIKSDMRLAKKIQTKLLPESGIQFQGIQCEIVYAP
                     MFEIGGDIYDIFQIRDGLIRVFLADATGHGIQAALMTTLIKGEFDKVKKQYSSPAEIL
                     RILNRNFLDSFRSLNVFFSCIICDINLNLREIVYSSGGHPEQAVLSQSGLTLLPKNGT
                     VIGIRDNAQFKDDRISFRPGSKLILFTDGLFEQFDREMQMFGEDRLSRIFEEMKDSNV
                     NAIREKCFSELKGFLEFENKFHDDITFIGIESL"
BASE COUNT          491 a          361 c          337 g          447 t
ORIGIN      
        1 cagagcttcc atcaagaatg ttcttgcgag atacaagatg attcagattc ccaagaagtc
       61 tgcagaactg gaacaggaaa tcctggaaac actggctgca agaatcaaga acagacatgt
      121 gctcattgag gatgtcaata ttgatgagac aaagcttgat cctggaatta tgaatgcaat
      181 tgctgagaaa cttaaaagag aacaggaact cgaaacaatg aagtacaatc agaaaattgc
      241 agaaaaggaa gacgagatag caaagatgaa agccaaaaga gatgcggaag tggaaatcat
      301 tgctgctgaa gcaaaggcca aaactcagag gcttacaaat gtgcacatca ctcctgccta
      361 tctgaaactg aagacggtgg aagccagcat gaaggcgctg gaaagtccga atgccaaact
      421 tattttcgtt ccggtaggga aagacggact tcctctgaat gtaaacattt ccgacaaaat
      481 cttcagtgaa tcaggattcc ctgcgcttcc ggcagagaag aaacactgag aatcagagcg
      541 attctattcc gatgaatgta atgtcatcat gaaatttgtt ttcgaattca aggaaacctt
      601 tcaattccga aaaacacttt tcacggatgg cgtttacatt gctgtctttc atttcttcaa
      661 aaatcctgct gagtctgtct tctccgaaca tctgcatctc tctgtcaaac tgttcgaaaa
      721 gtccgtctgt aaaaaggatc agctttgatc cgggtctgaa actgattctg tcatccttga
      781 actgagcatt atcccgtatc ccaatcactg ttccgttttt cgggagcaga gtcaatccgg
      841 actgactgag aactgcctgc tcgggatggc ctccggaaga atatacaatc tctctcagat
      901 tcagattgat gtcgcagatg atgcaggaaa aaaatacatt cagtgaacgg aagctgtcga
      961 gaaaattcct gttcagaatt ctcaatattt cagcaggact ggaatactgc tttttcacct
     1021 tgtcaaactc gcccttgatc agggtcgtca tcagtgcagc ctgaatcccg tgacctgtcg
     1081 catctgcgag aaacacgcgg atcagtccgt ccctgatctg aaaaatatcg taaatgtccc
     1141 ctccgatctc aaacataggg gcgtacacta tttcacactg aattccctgg aactgaattc
     1201 cgctttcagg aagaagtttg gtctgaattt tttttgcaag acgcatatca ctctttattt
     1261 cacggatgat actgttcagt ctttcattcg tataattcag ttcctttgtc ttgttcttga
     1321 attcagtttc gagctgctca tacagaaccc cgctttcaat cgcaagggca atctgagaac
     1381 tgaatatttc cacagtgtca atgtctatcg cgtcaagtga actgctcaaa tatgaggagt
     1441 ccatataaaa cacgccgact atggaattct gtatcatcag aggagatgca attacaatga
     1501 taaattcctt ttctttaccg taacgatatt cggaaaatct tctgatctga gtttttttct
     1561 cctctattgc atccgtcatc accctgagac tcgcctgaaa ggcagcatcc tgaatttcgg
     1621 attcggaaat atgtac
//
LOCUS       DAOQGJ010001050         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55295_length_1635_cov_2.283544, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001050 DAOQGJ010000000
VERSION     DAOQGJ010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55295_length_1635_cov_2.283544"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK683_14945"
     CDS             complement(<1..396)
                     /locus_tag="PK683_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNH09791.1"
                     /translation="MGLLELKNLFSKHDTMDLDKASPVNKNARGIPVPVKSGCSACRD
                     CENLCPTKAVRIISENRIEFNYGSCLQCGICTEVCSDSVLKNSGFNYVFALNRDDLTV
                     CFTEGEFTPAESEIPDNVKIFRELSGKRGF"
     gene            complement(396..>1635)
                     /locus_tag="PK683_14950"
     CDS             complement(396..>1635)
                     /locus_tag="PK683_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal (Ni/Fe) hydrogenase large subunit"
                     /protein_id="HNH09792.1"
                     /translation="EDYSILDNYRSMNDRERELAVCINSDGTLKNLDYHQLRIPVDSR
                     SYSHPVGPIHAGIIEPGHFRFYVTGEYIQMLHIRLGYQKRNLYRFLKNRSALQVMPVI
                     EAAASDSTVSYATAGAEIFEQSAGISISQDMNLFRKVLLEVERIAIHVGDIGAIAGDI
                     GWYPLLGICATDRGIPLGVMESLTGSRFGKSAIFPGQMKINRNLTKDDLQRLSGRVKE
                     VYDRVSREIRRTLLSTTVRERLHDCGLISKTQVYENGFIGMAARCTGAVQDLRLSQKI
                     YTETGLTLWNEEHSDQLTGDAWSRLYLRYVEFQNSCEWLMKVIPSLNVNISPEEGFSI
                     LKRKDFQPGLYFSSVEGWRGPVLMALEISKEGKVLDSYIRDPSVLNWHALELAVRGEL
                     IGDFPLNNKSFNLSYIGVDL"
BASE COUNT          411 a          413 c          327 g          484 t
ORIGIN      
        1 gaagcctctt tttcctgaaa gttctctgaa tatttttaca ttatccggta tctctgattc
       61 cgctggagtg aattctccct cagtaaaaca gactgtgaga tcatctctgt tcagagcgaa
      121 tacataattg aatcctgaat ttttcagtac actgtcactg cagacctcag tgcagattcc
      181 gcactgcaga catgacccgt aattaaattc tatcctgttt tctgaaatga ttctgaccgc
      241 tttggtcggg cataaattct cgcagtctct gcaggcagaa cagccgctct ttacaggcac
      301 aggaatcccc ctggcatttt tattcaccgg agatgccttg tccagatcca tcgtatcatg
      361 ttttgaaaaa agattcttaa gttccagaag tcccattaca gatccactcc tatataactc
      421 agattaaatg atttattgtt caatggaaaa tctcctatca gttcgcctct gacagcaagt
      481 tcgagtgcat gccagttcag aacggaaggg tcccgaatat aactgtccag aacttttcct
      541 tcttttgaga tttccaatgc catgagcacg gggcctctcc agccctcaac gctggaaaaa
      601 taaagtcccg gctgaaaatc tttccttttc agaatactga atccctcttc gggagaaata
      661 tttacattca gagacggaat gactttcatc agccattcac agctgttctg aaattccaca
      721 tatcggagat aaagtcttga ccaggcatcg cctgtcagct ggtcagaatg ttcttcattc
      781 catagagtga gtccggtttc cgtatagatc ttctgagaaa gacggagatc ctgaactgcc
      841 ccagtgcatc tcgcggccat tccgatgaag ccgttttcat atacctgagt cttggaaatc
      901 agtccacagt catgaagcct ttctctgacc gtagtggaaa gaagagtcct tcttatctct
      961 cttgataccc tgtcgtacac ttcttttact ctgcctgaaa gtctctgcag atcatccttg
     1021 gtcagattcc tgtttatctt catctgtccc ggaaaaatgg cagattttcc gaaacgggaa
     1081 cctgtcagag attccatcac gcccagagga atccctctgt ctgtggcaca gatccccaga
     1141 agaggatacc agccgatgtc tcctgcaatt gcaccgatgt ctcccacatg gattgcaatt
     1201 ctttccactt ccagaagaac ctttctgaag aggttcatat cctgagaaat gctgattcct
     1261 gcagactgtt caaatatttc tgcaccggct gtcgcatagg atacagtgga gtcgcttgct
     1321 gcggcttcga tgacaggcat aacctgaagt gcagaccggt ttttcaggaa ccgatataga
     1381 tttcttttct gatatcccag tctgatatgg agcatctgta tatattcacc agtcacataa
     1441 aaccggaaat gtcccggttc aatgattcct gcatggatcg gtcctacagg atgcgaatag
     1501 cttctgctgt ctaccggaat ccgcaattga tgatagtcca ggttcttcag tgtcccgtct
     1561 gaatttatgc agactgcgag ctccctctcc ctgtcgttca tgcttctgta attatccagt
     1621 atgctgtagt cctct
//
LOCUS       DAOQGJ010001051         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55313_length_1634_cov_5.146295, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001051 DAOQGJ010000000
VERSION     DAOQGJ010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55313_length_1634_cov_5.146295"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PK683_14955"
     CDS             complement(<1..243)
                     /locus_tag="PK683_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09793.1"
                     /translation="MKKLFIILIISQFQLFNTSVHAQTAPGEQKDTPEYKMMEMRAEL
                     DSLKKSNMENAAKIKAEAEQSRKSADMLWVTLCAALV"
     gene            complement(240..>1634)
                     /locus_tag="PK683_14960"
     CDS             complement(240..>1634)
                     /locus_tag="PK683_14960"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH09794.1"
                     /translation="GTISVESNTEFFYKEDDLEILEALGSQLALALTNVRLIEGMMEA
                     RKREAVVLSQLEWDLKMGRKVQSQIIQSSIAPWNGINFASFYEPMTEVSGDYFGIVKT
                     GNSLTFISADVSGHGIPAALVTMAIHSYFQRSVAQGMGLPEIMEYLGKSLKPQLPEST
                     YFTAFIIRIHSDYTFSYINGGHQTPLYFEATGETGELDSDGPPLGILDVTKSDYSEKF
                     GKFNSGDALLLLTDGFPEQKNLEREEFSLASMRKIFLDEKYSQLREKGKFNPEESIAG
                     MLKTWNDFKGEAPKGDDLSMILLYCNPSFNKAQTLFRSSKTALKEGDHEKTYELALSA
                     YNEDPSFSDNLLFLAKMYFNDKRYMESCKYLSEYIQTSGEDTAQIHYMLGKALFYAER
                     YEEAKISLRKSISCDHTYVKSNLFLAKCYLKEKAYTKAIRTIERGLKSAPNDEHLLNS
                     LERLNKKYGQGVPK"
BASE COUNT          420 a          365 c          318 g          531 t
ORIGIN      
        1 aacgagagct gcacagaggg tgacccagag catatcggca gactttctgc tctgttctgc
       61 ttctgccttg atttttgcag cattttccat attgcttttt ttcaaagaat cgagttctgc
      121 tctcatctcc atcattttgt attccggtgt atctttctgc tcaccgggtg ccgtctgtgc
      181 atgaacagaa gtattgaaga gctgaaactg cgatataatc agaatgataa aaagtttttt
      241 cattttggaa ctccctgtcc gtatttttta ttcagccttt cgagagaatt cagaagatgc
      301 tcatcatttg gagcactctt cagacctctc tcaattgttc tgattgcctt tgtataagcc
      361 ttttccttca gatagcattt cgccaggaaa agattcgatt ttacgtatgt atgatcgcag
      421 gatatcgact ttctgagtga aattttcgcc tcctcatacc gttctgcgta aaacagagcc
      481 ttgccgagca tataatggat ctgggctgtg tcttccccgc tggtctgaat gtattcagaa
      541 agatacttac atgattccat atatctttta tcattaaaat acatttttgc caaaaacaga
      601 agattatctg aaaatgaagg atcttcattg taggcactca atgcgagttc gtatgttttc
      661 tcgtgatctc cctctttcag tgcagtcttg ctgcttctga aaagagtctg tgccttgttg
      721 aatgagggat tgcagtaaag aaggatcatg gaaagatcat cccctttcgg agcctctcct
      781 ttaaaatcat tccatgtctt aagcattcct gctatgcttt cttccggatt gaattttcct
      841 ttttctctga gctgagaata tttttcatcc agaaatatct ttctcatgga tgcaagactg
      901 aactcttctc tttccagatt tttctgttca ggaaaaccgt ctgtgagaag aaggagagca
      961 tctcccgaat tgaatttccc gaatttttca gaataatcac ttttggtgac atccagtatc
     1021 cccaggggag gtccgtcaga gtccagctcg cctgtttcgc ctgtcgcttc gaaatacagc
     1081 ggagtctgat gacctccgtt aatataagaa aacgtataat ctgaatgaat tctgatgata
     1141 aaagcagtaa aataggtaga ttccgggagc tgcggtttca gggactttcc caaatattcc
     1201 ataatttcag gaagacccat cccctgtgcg acagatcttt gaaaatagga atgaattgcc
     1261 attgtgacaa gagctgccgg aatcccatgt ccggaaacat ctgctgaaat gaatgtaaga
     1321 gagttgcctg tctttacaat tccgaaataa tccccgctta cttcagtcat cggttcatag
     1381 aaacttgcaa aattgattcc gttccaggga gcaatgctgc tctgaataat ctgactctgc
     1441 acttttctgc ccattttcag atcccattcc agctgagaaa gaacaaccgc ttctcttttt
     1501 cttgcttcca tcattccttc gatgagtctg acgttcgtca gtgcaagagc cagctgtgac
     1561 cctaatgctt ccagaatctc cagatcatct tctttgtaga aaaattcagt attgctttcg
     1621 actgagattg ttcc
//
LOCUS       DAOQGJ010001052         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55346_length_1634_cov_2.801773, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001052 DAOQGJ010000000
VERSION     DAOQGJ010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55346_length_1634_cov_2.801773"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="PK683_14965"
     CDS             <1..1325
                     /locus_tag="PK683_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNH09795.1"
                     /translation="EAAKKIIENNTERKNKSIFTSNADGEKILITECSSEAEEAAFII
                     NAIQKYQKVEGSYRGMAVFYRTNAQSRFFEEALRNKSIPYVIHGGFRFFDRMEIKDMI
                     AYLCVLVNPDDSMSLMRIINYPPRGIGETSIEKIRKKSLDENRPILDILKKGDLDMRK
                     ASLKSVQEFTANIEELQTQLRTGIMPSIVSANLLKLMKISEHLSGMKDFESADRLENI
                     NQFILSIQEYEENTENPSLEEYLNQISLLTSEEAEKDLEDFVTLMTIHNSKGLEFDTV
                     FLAGMEEGTFPHSMSIQDDNIEEERRLCYVAVTRARKKLFLSYCQSTRKFGDFQFRIP
                     SRFLDELPKDLIYRNRGNSVRASEIRTPFASNRKSNPKEEAKPKSTVTAQGRTGEKYT
                     VGAKVSHKDYGTGKIISISGTGDNQKIKVQFGFSEKSFLAAYTNLEVL"
     gene            1419..>1634
                     /locus_tag="PK683_14970"
     CDS             1419..>1634
                     /locus_tag="PK683_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09796.1"
                     /translation="MGTSPAIPKPTLEDFDAAFDVIVTKSQDRTLRMLKLDMQIPEEY
                     ERDMKGFLSKYFKNDPFDIRIEDFEEII"
BASE COUNT          586 a          301 c          353 g          394 t
ORIGIN      
        1 tcgaagctgc aaagaagatc atagaaaaca atacggagag aaagaacaaa tccatcttta
       61 catccaatgc agacggtgaa aaaattctga ttactgaatg cagttcggag gcagaagagg
      121 ctgcttttat tataaatgca atacaaaaat atcagaaagt cgagggaagc tacagaggaa
      181 tggctgtgtt ctacagaaca aacgcacagt ccagattctt tgaagaggca ctcagaaaca
      241 agtccatccc ttacgtgatt cacggaggct tccgcttctt tgacagaatg gaaatcaagg
      301 atatgattgc atatctctgt gtacttgtaa atcccgacga ttcaatgagt ctgatgagaa
      361 tcatcaacta ccctcctcga ggaatcggtg aaacaagcat tgagaaaatc agaaaaaagt
      421 cactcgatga aaaccggccg attctggata ttctgaaaaa aggagacctg gatatgagaa
      481 aggcatcatt aaaatctgtt caggaattca cagcaaatat agaagaactg cagacacagc
      541 tcagaacagg aattatgccc tcgattgttt cagcaaatct tctgaaactg atgaagatct
      601 ctgagcatct ttcaggaatg aaggactttg aatctgctga cagactggaa aacatcaacc
      661 agttcatact ctccattcag gaatatgagg agaatactga gaatccttct cttgaggaat
      721 atctgaatca gatcagtctt ctcacgtcag aagaggcaga aaaggatctg gaagattttg
      781 tgacactgat gacgatccac aattcgaaag gactggaatt cgatactgta ttccttgcag
      841 gaatggaaga gggaactttc ccccattcga tgagcattca ggatgacaat attgaggaag
      901 aaagaagact ctgttatgtt gcagtgacta gggcaaggaa aaaactcttt ttaagctact
      961 gtcagagcac tagaaaattc ggagacttcc agttcagaat cccaagcaga tttctggatg
     1021 aacttccgaa agatctgatt tacagaaaca ggggaaattc agtcagggct tctgaaatca
     1081 ggacaccctt tgcatcaaat cgaaaatcga atccgaagga agaggccaaa cccaaatcaa
     1141 cagtcacagc acagggaaga accggtgaaa aatacacagt aggtgcaaag gtcagtcata
     1201 aagattacgg tacaggaaaa ataatatcaa tctcaggaac cggagacaat cagaagatca
     1261 aagtacagtt cggtttttcc gagaaaagtt ttcttgctgc atatacaaat ttggaagttt
     1321 tatagaagca gaaaaacttg cgcaactaaa atttttatcc ttcaggattt agttgaaaaa
     1381 tctgtttaat ttcagaatgc tgctaaagag agggaaatat gggaacgagt ccggcaatac
     1441 caaaaccgac attggaagat tttgacgctg catttgatgt tattgtcaca aaatctcagg
     1501 acagaaccct cagaatgctg aaactggata tgcagattcc ggaagaatac gaaagagata
     1561 tgaaaggatt tctttccaag tatttcaaga atgacccgtt tgatatccga attgaggact
     1621 ttgaagaaat catt
//
LOCUS       DAOQGJ010001053         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55347_length_1634_cov_2.795440, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001053 DAOQGJ010000000
VERSION     DAOQGJ010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55347_length_1634_cov_2.795440"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PK683_14975"
     CDS             <1..937
                     /locus_tag="PK683_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH09797.1"
                     /translation="SPSSVQKLGKRALQEIFSSLKNDSSSSSRFTKETGDSENLIASN
                     RALEFGDSTSKIDYISSIQNSVFRGNGRRPSIMDMEVSLARGTSRSGTVVLIDMSGSM
                     ARSSRFFNAKKVTLAIDALIRSEYKDDQLTIVGFGTLAKIIPASKVPSLQPYPVTIFD
                     PYIRLRYNMNKMGEKEKEELIPLYFTNLQKGLKTARQILSSRQIRNKNIILITDGAPT
                     AHFKGSILEVNYPPAPSDFEEALREASLCREDNITVNTFLLTSEWDYNYFGEESFIRQ
                     FAKSVEGRIFYPHPKELGKMILFDFLENRKKKFSY"
     gene            1005..>1634
                     /locus_tag="PK683_14980"
     CDS             1005..>1634
                     /locus_tag="PK683_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09798.1"
                     /translation="MENTLKYLPPISKYHRKISLDDWFESTKYYDQGNYYKSLIHLLR
                     YINGPVPIPDTPSISLKIPHGSVYVNIQADAEKFKVSVPFLKFIPGSVKVAAMRQMIE
                     QNFSTLILGQIVLKGEELWYEYEDKTSNCEPYKIYYMLDELCTQADNNDDYYIDRFKL
                     DHVEKPVLDQFTPTEYSKAESEFKEIISEGLKYADYFEKNRWYNLTCDVL"
BASE COUNT          514 a          371 c          328 g          421 t
ORIGIN      
        1 ctctccgtct tccgttcaga aacttggaaa aagagccctt caggaaattt tttcctcact
       61 gaaaaacgac agcagttcct catcccgatt caccaaggag acaggagact ctgaaaacct
      121 gattgcctcc aaccgtgccc ttgaattcgg cgacagcact tccaaaatcg actacatatc
      181 gtccattcaa aactctgtat tcagaggcaa tggaagaaga ccttccatta tggatatgga
      241 agtctctctt gccagaggca cctcacggtc aggcacagtc gttctgattg atatgagcgg
      301 ctctatggcg agatcatccc gattcttcaa tgcaaaaaaa gtgacccttg caatcgatgc
      361 actgattcga tccgagtaca aggatgacca gctgacaatc gtcgggttcg ggacattggc
      421 aaaaattatt cctgcttcca aggttccgtc acttcagccg tatccagtca ctatctttga
      481 tccttacatc cggctcagat acaatatgaa caaaatggga gaaaaggaaa aagaagaact
      541 catccctctt tatttcacaa atctgcaaaa aggactgaaa actgcgagac agatcctctc
      601 ttccaggcag ataaggaaca aaaacatcat attgattaca gacggtgcac cgacggccca
      661 tttcaaggga agcattcttg aagtcaacta ccctcctgcc ccctcggatt tcgaagaagc
      721 tctcagggag gcctctctgt gcagggaaga caatatcaca gtcaatacat ttcttcttac
      781 gtcagaatgg gattacaatt atttcggcga ggaaagtttc atccggcagt ttgccaaatc
      841 agtggaagga agaatattct acccccatcc taaagaactg ggaaagatga ttttgttcga
      901 ttttctcgaa aatagaaaga aaaagttcag ttattgaaag aaaaagcaaa aaaactgttt
      961 tagcttgcca aaaacggtct ttccgcttgt atgtattttt tgttatggag aatacgctta
     1021 aatatctacc acctatttcc aagtaccaca gaaaaatatc tctggatgac tggttcgaat
     1081 ctacaaagta ctatgatcag ggcaattatt ataagtcact gattcacctt ctcagataca
     1141 tcaacggccc tgtaccgatc cccgatacgc cttcgatcag tctgaaaatc cctcacggtt
     1201 ccgtttatgt aaatattcag gcagatgcag agaaattcaa ggtaagtgtt ccttttctga
     1261 aattcattcc gggctccgtc aaagtagctg ctatgagaca gatgattgag cagaattttt
     1321 ctactctgat cctcggtcag atagtgctga agggagaaga actctggtac gaatacgaag
     1381 acaagacatc gaactgtgaa ccgtacaaaa tttattatat gcttgatgag ctctgcactc
     1441 aggcagacaa taatgacgac tactatatag acagattcaa gctggatcat gtcgaaaaac
     1501 cggtattgga tcagtttacc ccgacagaat acagcaaggc agaatccgaa ttcaaggaga
     1561 tcatttcaga aggtctgaaa tatgccgatt actttgaaaa aaacagatgg tacaacctga
     1621 cctgcgatgt tctg
//
LOCUS       DAOQGJ010001054         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55395_length_1633_cov_3.382763, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001054 DAOQGJ010000000
VERSION     DAOQGJ010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55395_length_1633_cov_3.382763"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PK683_14985"
     CDS             complement(<1..319)
                     /locus_tag="PK683_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNH09799.1"
                     /translation="MKTLEIPAFGLENLRIAEREIPVPKKGEVLVKIKAVSLNYRDYL
                     TVIGKYNPKYSLPLVPGSDCAGEVVEAGEGVTEWKLGSRVMGNFAQSWITGTPDFRAL
                     RKST"
     gene            complement(731..1507)
                     /locus_tag="PK683_14990"
     CDS             complement(731..1507)
                     /locus_tag="PK683_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09800.1"
                     /translation="MKKSILKISVSVFLFSSAFSLSANAFKGTWENTASPIQPYINIE
                     DGSHLQARFIGMGGPRYSADCKAQGDSLHYTITEGMMPKKDKMIDIVGQKGVCKIEKV
                     ADHIYFQESLKCTGPFEEIQKSLQKGEGGLDEKIDIKDLKVFRTYLPKNTPRKIQGTD
                     TLSILKKGKVNDAAYLRSAPDVSAKPLNCLIRMPESTGKPEYEGQVIAKGREIFVIAK
                     AKTKTKVQKWDNYWYYVESNPYGGKNCGPGWMFGEFITIE"
BASE COUNT          450 a          360 c          320 g          503 t
ORIGIN      
        1 gggtggattt gcggagtgca cgaaaatcag gagttcctgt gatccagctc tgagcgaaat
       61 ttcccatcac tcggcttccc agtttccatt cagtcacgcc ttctcccgct tcaaccactt
      121 cccccgcaca gtcgcttccc ggcacaagag gaagagaata cttcgggttg tatttgccga
      181 ttacagtcag gtagtccctg taatttaaag atacagcttt gattttgaca agcacctcgc
      241 cttttttagg cacgggaatt tcccgttctg ctattctgag gttttccagt ccgaaagcgg
      301 gtatctctaa agttttcatt gtcctgtcct gtaaaagagt attagtgcga tattgtcaag
      361 tcggccaaaa cttaaaagtc ttttttatgg aactgagtaa acgctgaatt tcaccgcgaa
      421 gggcacgacg gatgcaaagg aatcaggcgt ctgataacag aagaccttct ccttgcagac
      481 agcaagcatt cccatttttc aatcagtgag attaattatt tgagaattat aatactgccc
      541 aaaatccagt acatgacaaa gtcgcactaa ttcagaacaa gacaggcttt ttcatagagc
      601 ggggcggctg atgcagaaga tagacaccgt aggattatga ctcacctcag tctgaatgaa
      661 tctgaaaagt gatttattat ttatcattat tttatatcca aaataatgga taatacggaa
      721 tcaatcaatt ttactctatt gtaatgaatt ctccaaacat ccagcccggc ccgcaatttt
      781 tacctccata cggatttgat tccacataat accagtagtt atcccatttc tgaactttgg
      841 tctttgtttt tgcctttgca atgacaaata tctccctgcc ttttgcaatg acctgtcctt
      901 catattccgg tttgccagta gactcaggca ttctgatgag acaattcaga ggttttgcag
      961 aaacgtccgg tgcagaacgc aagtaagctg catcattcac ttttcctttt ttcaaaatgg
     1021 aaagtgtgtc tgttccctga atctttctgg gtgtattttt cggaagatat gtccggaata
     1081 cttttaaatc tttgatatca atcttttcat ccaggccgcc ttctcctttt tgcaggcttt
     1141 tctgaatctc ttcgaagggc cctgtgcatt tgagactttc ctgaaaataa atgtggtcgg
     1201 cgaccttctc aattttgcag acaccttttt ggccgacgat atcaatcatc ttgtctttct
     1261 tcggcatcat tccttcggta attgtatagt gcaggctgtc tccctgagct ttgcagtcag
     1321 ccgaatatct ggggccgccc ataccgataa aacgagcctg caaatgtgac ccatcttcaa
     1381 tgtttatata aggctgaatt ggagatgctg tattttccca agtacccttg aacgcatttg
     1441 cagaaagaga aaaggcagag gaaaacagga aaactgatac agatattttg aggattgatt
     1501 ttttcatagg gtcaaagtaa acagaatatt attccaggtc aatctaatgt gttgacagcc
     1561 ggacaaaatt ttatcagtct ctgcatcaga ttctccaaaa aaatctagac agattaaaaa
     1621 tacaaggtat cct
//
LOCUS       DAOQGJ010001055         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55397_length_1633_cov_3.320659, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001055 DAOQGJ010000000
VERSION     DAOQGJ010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55397_length_1633_cov_3.320659"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..688)
                     /locus_tag="PK683_14995"
     CDS             complement(62..688)
                     /locus_tag="PK683_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09801.1"
                     /translation="MKISWKNTFIVVLLLQLSYIPCDVSGNNTSHSEKSHKSRFRVLD
                     LSNYRSLDKFTGWGGAPGSLDENSSPENTEPKDWFYSKIKAKASIKSIQTNSHSMKIN
                     TCQSAAEINGVSSSFQSFLNEWLTENSPDLKKGKITLSVPENRTSYTCSFQREGNSLT
                     QECSGSLKGHGIAGCWTLEGRDWEECECITYIHIPGGKASLRNRIRLN"
     gene            complement(688..>1633)
                     /locus_tag="PK683_15000"
     CDS             complement(688..>1633)
                     /locus_tag="PK683_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH09802.1"
                     /translation="VINTTGVHIDEIRKMDDPSAKNVLSPSQGIHLVFKRDKILCKSA
                     MIIPKTSDGRVVFVIPWEDCTIVGTTDTPVKTIQDEPMPLNEEVDFVLKTVQEYLGVR
                     VEKKDVISIFAGLRPLIAPDGSQNTKNISREEMILISPSGLITMSGGKWSTYRKMAED
                     LVDKVVLEGKLTPDTKCNTYDYAFPGKKGYSENLYIDIAQIYSVSQDQAKRLRNYYGG
                     LTSEILGKTPKEILPGTGLFEEEVRYFAESEFALGISDVIARRWRTAFTNLELAKKLV
                     TPVGKILSNSLGWTQAFLKSEEKSALDWMESLEKSIGK"
BASE COUNT          414 a          356 c          334 g          529 t
ORIGIN      
        1 attgaaaggg ttttagatca agttatgcag tcaaccggaa aaaacgtctg attttgatgg
       61 atcagttcag tctgatcctg ttcctgagag aggcttttcc acccgggata tggatatatg
      121 tgatgcattc gcattcttcc cagtctcttc cctccaaagt ccagcatcct gctatgccgt
      181 gaccttttaa agatccggaa cattcctgag tcaaggagtt tccttccctt tgaaaactgc
      241 aggtatagga agttctgttc tcaggaacag agagagtgat ttttcctttt ttcagatcag
      301 gactgttctc tgtaagccat tcattcagaa aagactgaaa agaagaactg accccattga
      361 tttctgcagc agactgacag gtatttatct tcattgaatg ggaatttgtc tgaattgatt
      421 tgatggatgc ttttgctttt atttttgaat agaaccagtc ctttggttct gtattttcgg
      481 gactggagtt ttcatcgaga gagccgggag ctcctcccca gcctgtaaat ttgtccagag
      541 atctgtaatt ggaaagatcc agaactctga atctggattt gtgggatttt tccgaatggg
      601 aagtattgtt tccggatacg tcacatggaa tgtaagacaa ctgcagaaga agaacgacga
      661 taaatgtgtt tttccacgaa attttcatta ttttcctatg gatttctcca ggctttccat
      721 ccaatccagt gcagattttt cttctgactt caggaatgcc tgtgtccatc ctaatgaatt
      781 agacagaatt tttcctaccg gagtcacaag ttttttggca agctccagat tcgtaaatgc
      841 agttctccat cttctagcga tcacatccga aatccccaat gcaaattcac tttccgcgaa
      901 gtatctgacc tcttcttcaa aaagcccggt tcctggaaga atttcctttg gagtttttcc
      961 cagtatttcg gaagtcagtc cgccatagta atttctcagt cttttggcct gatcctgaga
     1021 aacactgtaa atctgagcga tgtcaatata gagattctct gagtaccctt tttttcctgg
     1081 gaaagcataa tcgtaagtat tgcattttgt atcaggagtg agttttcctt ccaatacaac
     1141 cttgtctaca aggtcctcgg ccatttttcg gtaagtagac cattttcccc cgctcattgt
     1201 aatcagtcca gacggagaaa tcaggatcat ttcttctctc gaaatattct tggtattctg
     1261 gcttccgtcc ggtgcgatca gaggacgcag tcctgcaaaa atagaaatca catccttttt
     1321 ttccactcgt acacccagat attcctgaac tgtcttcagg acaaaatcca cttcctcatt
     1381 cagaggcata ggctcgtctt gaattgtttt gacgggtgtg tcagtcgttc cgacaattgt
     1441 gcagtcttcc cagggaatga caaaaacaac tcttccgtca gatgtcttgg gaatgatcat
     1501 tgcacttttg cagaggatct tatctctttt gaataccaag tgaattccct ggctggggga
     1561 aagtacattc tttgcagaag ggtcgtccat ttttcggatt tcatcgatat gaactcctgt
     1621 ggtattgatc aca
//
LOCUS       DAOQGJ010001056         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55408_length_1633_cov_2.903676, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001056 DAOQGJ010000000
VERSION     DAOQGJ010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55408_length_1633_cov_2.903676"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..1083)
                     /locus_tag="PK683_15005"
     CDS             complement(358..1083)
                     /locus_tag="PK683_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09803.1"
                     /translation="MNGQAVLENLKNEYYLEKSFFIEEIEGLQLWRLGLDSRDEFNRF
                     IFKVYSEAFGTDGILPFSFEDIETQSALYYSKTKVCGIRHPDGTLLGTWGLVLKNVEK
                     DRFLLPIEANYSLSSAEIIRIMGEKNIQYIFNGWRTAIDKEAMERLNLPRQKSIFIFD
                     FLLRGLTKDFTGSSDTYLGVSEMEKLVYKYHRRVGIPWVILAEPKEYWGRDRYPCAFR
                     MGEFQDYLKKNHPERFVFLTGRS"
     gene            1338..>1633
                     /locus_tag="PK683_15010"
     CDS             1338..>1633
                     /locus_tag="PK683_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH09804.1"
                     /translation="MTDTKTKILETASELFYSKGYVNTGVEEIITLCQIKKPTLYYYF
                     KSKTDLGLAYLESKETEFIGVLIKLGQRTKTAHEFFAAWTTLVKRSARENNFHG"
BASE COUNT          474 a          372 c          312 g          475 t
ORIGIN      
        1 cactttacgg catactctgt gcgccaatgc cataagccgg ctgaacttcc gttctttctg
       61 attcatcaga gacttttata ctgccggctc atttgacgct tcctgaaaac tataggtccc
      121 atgaagtata ttgcttttgt gcaagatcaa taatacacag atttccaata acggtatgtc
      181 gcaggtttgg aatgatctgt catcctgagt ccgtcgagga agctgttccg agagtcacag
      241 ttcgactctg ttctcactgt cataatgacc gtaattgcac agtcggctgc agtcttctcc
      301 aagtatgaat ccatcatacc tcataagcgg aaaaaagctg agacagtttt tgaaaattta
      361 tgacctgcct gtcagaaaga caaatctttc aggatgattt tttttcagat agtcctgaaa
      421 ctctcccatt ctgaatgcac agggatacct gtcccgcccc cagtattctt tgggctctgc
      481 aagaatcacc caggggattc ccactctgcg atgatatttg tacacaagtt tttccatttc
      541 agaaacaccc agatatgtat ctgagcttcc tgtaaaatct ttagtcagcc ccctgagaag
      601 aaaatcaaag ataaaaatgg atttctgcct gggcagattc agtctttcca ttgcctcttt
      661 gtcaatggca gttctccatc cgttgaagat gtactgaata tttttctctc ccataattcg
      721 aataatctcg gctgagctta agctgtagtt tgcttcaatg ggaagaagaa aacggtcttt
      781 ctcgacattc ttcagaacaa gtccccaggt tcccagaagt gttccgtcag gatgtctgat
      841 cccgcatacc ttagttttgg aataatacag ggcgctctga gtttctatat cctcgaagct
      901 gaaagggaga attccgtctg tcccgaacgc ctcactgtac actttgaaga tgaatctgtt
      961 gaattcatcc ctgctgtcaa gacccagtct ccaaagctga agcccttcta tttcttcaat
     1021 gaagaacgac ttttccagat aatattcatt tttcaaattt tccaaaactg cctgaccgtt
     1081 cataagaatc tcctgcaata gtttccagat caatataccg aacggtaggt ataaagtcaa
     1141 cagaaaaatt ccgtgactgc ttgcgcttct gcacttgcga caatgcgctt tcagaaatct
     1201 gatcctaatt atataattta tccttttata attatatttt atccgtaaac ggaccgattt
     1261 acggatattt tcttgacaga aaatcctgtc cccggataaa gtaaggtttc ctgcatgaaa
     1321 aggcaggcat tgagattatg acagacacaa agacaaaaat tctggaaacg gcttcggaac
     1381 ttttttattc caaaggatat gtgaacacag gtgtagagga aattatcaca ctttgccaaa
     1441 tcaaaaaacc cacgctttac tattacttta agtcaaagac agaccttggg ctagcctatc
     1501 tggaaagcaa ggaaacggaa ttcataggtg ttctgataaa actggggcag aggacaaaaa
     1561 cagcccatga attcttcgca gcctggacaa ccctggtaaa acgatccgcc agagagaata
     1621 attttcacgg atg
//
LOCUS       DAOQGJ010001057         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55409_length_1633_cov_2.856781, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001057 DAOQGJ010000000
VERSION     DAOQGJ010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55409_length_1633_cov_2.856781"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PK683_15015"
     CDS             complement(<1..776)
                     /locus_tag="PK683_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09805.1"
                     /translation="MPLVRLFIFLNIIFLFSYCAAELKDSRPPSTAVKEQASTDTNKP
                     EAPVSPAGKLDGKKETEKKKEPVKDSKTVTESEEQHDNDSSDVSERPKSENSSSGLKA
                     GYADDNKQFNYFAGFLEKFKSVPHIDLNISERIFIKIKEKNGKPASGAKVRILSGGSE
                     IESGTAFADGTFLFFPSEHSGKLKDYKAVVSKNGAEKEISFKRDGQRNIETVLNVSPN
                     QTSYPMDIAFIMDTTGSMGEEINRLKSTIELINLNLSGMKG"
     gene            complement(799..1389)
                     /locus_tag="PK683_15020"
     CDS             complement(799..1389)
                     /locus_tag="PK683_15020"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HNH09806.1"
                     /translation="MAKIIQLKSQTGYQLKITLSSIKPEIWRRFTVSADIKLSDLSRV
                     IQTVMGWTNTHLHQFITKDRTLYAMPDEENSEFITDYRKVHLNQILTKVKQSIVYEYD
                     FGDGWEHKIVLEKILQDYKDKHPVCLAGRRECPPEDCGGPFGYQRLLDTISDPENPEY
                     EDVLDWLGDGWDPKFFDETEISEKLKKKNFGCVDLY"
BASE COUNT          422 a          379 c          317 g          515 t
ORIGIN      
        1 cccttcatcc ctgacagatt cagattaatg agttcaattg tggatttcag cctgtttatt
       61 tcctccccca tgcttccggt tgtatccatg atgaatgcta tatccatcgg gtacgaagtc
      121 tgatttggac tgacgttcaa aaccgtttca atgtttctct gaccgtctct cttgaagctg
      181 atttcctttt ctgctccgtt tttggaaact acagccttgt agtccttgag tttcccagaa
      241 tgctccgacg gaaaaaacag aaatgtcccg tcagcaaatg cggtacctga ctcaatttca
      301 cttcctcccg aaaggattct cactttggcc cctgaagcag gctttccgtt tttttcctta
      361 attttaatga aaatcctttc agaaatattc agatcaatat gaggaacaga cttgaacttt
      421 tcaagaaatc ctgcaaaata attgaactgc ttgttgtcgt cggcataacc tgccttcaga
      481 ccggaagagg aattttcaga cttcggcctt tctgagacgt cggaactgtc attatcatgc
      541 tgctcttcag actcggtcac tgtctttgaa tctttgacag gctctttctt tttctcagtt
      601 tccttttttc cgtccagctt tcctgcaggc gagactgggg cttccggttt atttgtatct
      661 gtcgacgcct gctctttcac tgcagtggaa gggggacggg aatccttcaa ttcagcagca
      721 cagtaggaaa ataaaaagat aatattcaaa aaaataaaca gtctgactaa gggcataatt
      781 attagactcc ttttcggatc aatacagatc aacgcagccg aaattctttt ttttcagttt
      841 ttcgctgatc tctgtttcat caaaaaattt cggatcccaa ccgtccccca gccagtcgag
      901 cacatcctca tattccggat tttcaggatc agagatggtg tccaaaagtc tctgatatcc
      961 gaagggaccg ccgcagtcct caggtggaca ctctcttctg cctgccaggc agactgggtg
     1021 tttatcctta tagtcctgaa ggatcttttc cagaacaatt ttatgctccc acccgtcacc
     1081 gaaatcatat tcatatacta tcgactgctt tacctttgtc agaatctgat tcagatgaac
     1141 tttcctgtaa tctgtgataa attctgagtt ctcttcatca ggcatcgcat agagagtcct
     1201 gtccttggtg atgaactgat gcagatgtgt attcgtccag cccatcactg tctgaatcac
     1261 cctgctcaga tccgacagtt ttatatctgc actgacagta aatcttctcc atatttccgg
     1321 tttgatgctg ctcagtgtga ttttcagctg gtatcctgtc tgagatttca actgtatgat
     1381 ttttgccatt gtttagattc tcctgcggac tgtctgagga cagattatcc gactttctta
     1441 gaatttttca ggccggacag aagaaaatga aaaaatgatt ttgagaggaa aacaggaata
     1501 gactgaagaa caaaatcagc caggaataaa aaagagatca ttttgactga ttttgagcgt
     1561 tcgggactgc gtaaaagcaa aattgcaact ggtcagcata catataccca gatttctaat
     1621 ctcgcaaatc tgg
//
LOCUS       DAOQGJ010001058         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55423_length_1633_cov_2.501901, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001058 DAOQGJ010000000
VERSION     DAOQGJ010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55423_length_1633_cov_2.501901"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PK683_15025"
     CDS             complement(<1..428)
                     /locus_tag="PK683_15025"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNH09807.1"
                     /translation="MRATSEKLKRNNGKQCCRQKQAYEISSEKKRESAKHIKFTEELK
                     GLVTDLLKERHTEQMIIGVSTRKVKRSLEDQKAFRSYGTSKSAVSRNFTVRTRKKLAD
                     WLGRRLDTEYPFIMIDGISFRGRTVLIALGISKDGHKSVLG"
     gene            complement(620..>1633)
                     /locus_tag="PK683_15030"
     CDS             complement(620..>1633)
                     /locus_tag="PK683_15030"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH09808.1"
                     /translation="VLTPTSGSVSVQGKVSTLLELGAGFNPELSGMENIFFYGSINGI
                     KRKDMEKFVEPILEFADIGEYIFQPVKSFSSGMFVRLAFAVAVQIEPDILIVDEALAV
                     GDIFFQQKCSRRMLEMREKGITILFVSHDMSSVRNICSRVIFLYKGEIKEDGEPNGII
                     TDYYKIGDHADEEDHKLGSLQKTDNRENENLSLADFSINDIHGIKTESFKINETAVFR
                     ASVKSKKELNDIHPVIALKNRFGTVIFIGSSYNDKGTGFSFSENSVYSVEFQLKLSVE
                     AGLYSVDLSFGIPIEGKVNQAMNCFEIQNISGLTVIWDYENEIAPFYGLCSLELKTSL
                     IKS"
BASE COUNT          445 a          374 c          263 g          551 t
ORIGIN      
        1 ccgagaacgc tcttatgccc gtctttgctg attcccagag caatcaggac tgtcctgcct
       61 ctgaatgaaa tcccatctat cattataaaa ggatattcag tatccagtct ccttccaagc
      121 cagtccgcaa gcttcttccg tgtacggacg gtaaaatttc tgctcaccgc gctctttgat
      181 gttccgtatg atctgaatgc tttctgatct tcaagagatc gcttcacctt gcgtgtggaa
      241 actccgatga tcatctgctc agtgtgacgc tccttcagaa gatccgttac cagtcctttc
      301 aattcttctg tgaattttat atgctttgct gattctcttt tcttctcaga ggaaatctca
      361 tacgcctgtt tctgccgaca acattgcttg ccattgttcc ttttcagctt ctctgatgtt
      421 gctctcatcg aaaatcctgg ttgatgatat acgtagattt tagatatttc tcctagggtt
      481 aactttttgt agctcattct gcctcttact gcttcgcaaa ttctatttgg ccttcttgag
      541 ctcttcagtc aactccattt gttacactta ttaattgatc ctgccatatt ttttaatagt
      601 tatatttgaa tgttattatt tatgatttaa ttaatgatgt ttttaattca agactgcaca
      661 aaccataaaa aggggcaatt tcattttcat aatcccagat aacagtaagt cctgatatat
      721 tctgaatttc gaaacagttc atcgcctgat tgactttccc ttctattggt atgccaaaac
      781 taagatctac tgaataaagc cccgcttcca cagaaagctt tagctgaaat tcaacactat
      841 acaccgaatt ttctgaaaaa ctaaaccctg tgcctttatc attatacgag cttccgataa
      901 agattacagt tccgaatcgg ttttttaaag caatcaccgg atgaatatca ttcaattctt
      961 tcttggactt tacagaggcc cgaaagacag cagtttcatt aatcttaaag ctttcagttt
     1021 tgattccatg tatatcattg atactgaagt ctgccagaga cagattctca ttttctcggt
     1081 tatcagtctt ttgaagagat cctagtttgt gatcctcttc atcagcatga tctccaattt
     1141 tataatagtc tgtgataata ccgtttggtt ctccatcctc cttaatctcc cctttataaa
     1201 gaaaaatcac tctgctgcat atattccgca cagaactcat atcatgagat acaaaaagaa
     1261 ttgttattcc tttctctcgc atttcaagca ttctcctgga acacttctgc tgaaaaaaaa
     1321 tatctcctac agccagagcc tcatccacaa taagaatgtc cggttcaatt tggactgcca
     1381 ccgcaaatgc gaggcggaca aacattccgc tggaaaagga tttaacaggc tggaaaatgt
     1441 attctccaat atctgcaaac tctaagatag gctctacgaa cttttccata tcttttcttt
     1501 ttattccatt gatggaaccg tagaaaaaaa tattttccat tccggaaagc tctggattga
     1561 atcctgctcc cagttcaagc aatgtagaaa cttttccctg aacggagact gatccggatg
     1621 taggagtgag aac
//
LOCUS       DAOQGJ010001059         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55494_length_1632_cov_2.170577, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001059 DAOQGJ010000000
VERSION     DAOQGJ010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55494_length_1632_cov_2.170577"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PK683_15035"
     CDS             <1..1143
                     /locus_tag="PK683_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNH09809.1"
                     /translation="VIFVTVFYIGFALSFTELTVSIPNAGGPFAYAKTALGNTGGFIA
                     GFFTLTEFLLAPPAIAAALGAYLHSLIPSLSPELISFTAFILFLGLNLLGVEQTAKFE
                     LFVTGAAASGLLLYCVLVLPYSDIGLTFRKTDQFSWLEFMHAIPFAIWFYLAVEGVAM
                     AAEETENPKKDIPIGYSLGIGSLVLFAVSVLLITSGTGMAERLSKSDNPLPLVLSSLY
                     PDKIYIVKIFSVLGLAGIMASLLGIILGFSRQIYALARERFLPPFLADLNEKNGTPTK
                     AILAGGLFGLICLSVGRTDELITVSVLGAAGMYIVSLVSLFVLRKKQPELPRPFRTPF
                     YPYLPALTLAGGVICFLSVFLQKPYLAGGYFIFFFFNLILFKIFHN"
     gene            complement(1148..>1632)
                     /locus_tag="PK683_15040"
     CDS             complement(1148..>1632)
                     /locus_tag="PK683_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09810.1"
                     /translation="THLNFRFFPNQVSEQIAFCQRDLYKFLPYTMYAMATPYGSFPPE
                     KYENSLIQGTVGQSAYKNEIVFDYSNQLSFSPFDIRFDRFKVHRIHGHEQTIRKLVKD
                     LNSYGTDAFISDGDEHTLTVRDPNDLIALNIRKSFCFSGRDGVSKNVSICGMNIRGAD
                     "
BASE COUNT          394 a          379 c          347 g          512 t
ORIGIN      
        1 gtcatttttg taacagtatt ttacatcggc tttgcactga gtttcactga actgactgtc
       61 tcgattccga atgcaggcgg accttttgcc tatgcaaaga ctgcacttgg aaatacagga
      121 ggattcattg ccggattttt tacgctgaca gagtttctgc tcgcccctcc cgcaattgca
      181 gccgcactcg gagcctatct gcattcactg attccttccc tctcacccga actgatttca
      241 tttactgcat tcattctttt tcttggactg aatcttctcg gagtggaaca gactgcaaaa
      301 tttgaacttt tcgtcaccgg tgcagccgct tcaggactgc ttctctactg tgttcttgta
      361 cttccctatt ctgacatagg tcttactttc cgaaaaacgg atcagttttc ctggctggaa
      421 tttatgcatg cgattccttt tgcgatctgg ttttatcttg cagtggaagg agttgcaatg
      481 gccgcagaag aaactgaaaa tcctaaaaaa gacatcccga tcggatacag tctcggaatc
      541 ggaagtcttg ttctttttgc tgtgtctgtt cttctgatca cttcaggcac ggggatggca
      601 gaaagacttt ccaaatcgga caatccgctt ccattggtgc tttcatcatt atatccggat
      661 aaaatatata tcgtgaaaat attctctgtg ctcgggcttg cagggattat ggcgtcgctt
      721 ctgggaatca tacttggatt ttccaggcag atctatgcac ttgcacggga aagatttctc
      781 cctccctttc ttgccgatct gaatgagaaa aacggaacac cgacaaaggc cattctggca
      841 ggaggactgt tcggtctgat ctgtctgtcc gtaggcagaa ccgatgagct gatcaccgtt
      901 tccgttcttg gcgctgcggg gatgtacata gtaagcctgg tgagcctttt tgttctgagg
      961 aaaaaacagc ctgaactgcc aaggccgttc agaactcctt tttatcctta ccttccggca
     1021 ctcactcttg caggaggagt aatctgtttt ctgtcagtat tccttcagaa gccctatctg
     1081 gcaggcggat attttatttt ctttttcttt aatttgattc tttttaagat atttcataat
     1141 taacagatca gtcagcacct ctgatgttca taccgcagat gcttacattt tttgaaactc
     1201 cgtccctgcc tgaaaaacag aaactctttc tgatattcag cgctatcaga tcattcggat
     1261 ctcttactgt cagagtatgc tcgtctccgt cgctgataaa tgcatccgtt ccgtaggagt
     1321 tcagatcttt gacgagcttc ctgatggtct gttcatgacc gtgaatcctg tgaaccttga
     1381 aacggtcgaa ccgaatgtca aacggggaaa aactcaactg attggaatag tcaaatacaa
     1441 tttcattttt ataggcactc tgccccactg ttccctgaat cagactgttt tcatattttt
     1501 ccggcggaaa tgatccgtac ggagttgcca ttgcatacat tgtataagga agaaatttgt
     1561 aaagatctct ctggcagaat gcaatctgct ccgaaacctg attcggaaaa aagcggaaat
     1621 tcaggtgggt aa
//
LOCUS       DAOQGJ010001060         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55595_length_1630_cov_3.297778, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001060 DAOQGJ010000000
VERSION     DAOQGJ010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55595_length_1630_cov_3.297778"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK683_15045"
     CDS             <1..230
                     /locus_tag="PK683_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09811.1"
                     /translation="MGIFSIASGKSRCFLFVMLLMMDKNGWGTMLKRAGIVMNDPFSD
                     ESDWELISIVGLDRNAKEFIRGFKPRLKQNS"
     gene            281..928
                     /locus_tag="PK683_15050"
     CDS             281..928
                     /locus_tag="PK683_15050"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HNH09812.1"
                     /translation="MKLLSLNNRFVFNLIFAENPNLLKALLNSILQPENEDEIQQLHI
                     LIPDKLGTADSHIFPQISEIKAIDSKGRYFTAETDTIPEKLFLKEASYSADSEAAPEK
                     RIYSFHFLNRHIFNNFDYMSKYRIVELESSFKRKEDLQICIIEFAKFNKTINELSTSL
                     DRWLYFMKNSGSLKDDQEETLMENDLQIKNAIKSLKTASNNPENWVIQMRYGSQL"
     gene            complement(1104..>1630)
                     /locus_tag="PK683_15055"
     CDS             complement(1104..>1630)
                     /locus_tag="PK683_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09813.1"
                     /translation="TALSPSSLYSYGVFTATVIFIWVARSIASLSNPSLSEKDLEEKY
                     AKYKSSYSLIRSFAETEILKNPAVPTSILEDQVRQRNYSAARHTNLSAESIDRLIKKM
                     SLTDWGLLSNLCSNPNMEKDTLEYLASKTAKDFKLPREWAEFQEYLYPHILVHPNASA
                     EVKQKIKEFSEKSE"
BASE COUNT          524 a          313 c          329 g          464 t
ORIGIN      
        1 aaatggggat tttttccatt gcatcaggaa agagcagatg ttttcttttt gtaatgctcc
       61 tgatgatgga caagaacggc tggggtacaa tgctgaagcg cgcaggaata gttatgaatg
      121 atccgttctc tgatgaaagc gattgggaac tgatctcaat agtcggactg gacagaaacg
      181 ctaaggaatt tatcagagga tttaaacctc ggttgaaaca aaattcttag ttaaaataat
      241 gataaaatat cttttttatc atctgcactg agcatacaga atgaaactac tcagcctgaa
      301 caatcgcttt gtattcaatc tcatattcgc agaaaaccca aatttgctta aagctctgct
      361 taattcgatt ctgcagccgg aaaatgaaga tgaaattcaa cagctgcata ttctgatccc
      421 ggacaaatta ggaacggctg actctcatat ctttcctcag atttccgaaa tcaaagcaat
      481 agacagtaaa ggaagatatt ttacagctga gacggacacc ataccggaaa agctgtttct
      541 gaaagaagca tcttattcag cagacagtga agctgctcct gaaaagagaa tatattcttt
      601 tcatttcctg aacagacata tatttaataa ctttgattat atgtcaaaat atagaattgt
      661 tgaacttgag agcagtttca aaagaaaaga agatcttcaa atctgcatta ttgagtttgc
      721 gaaatttaat aaaacaataa atgagctgag cacttcactt gacagatggc tgtattttat
      781 gaaaaactca ggcagcctga aggatgacca ggaagaaaca cttatggaaa atgatctgca
      841 aataaagaat gcaataaaat ctcttaaaac tgcgtccaat aatcccgaaa attgggtaat
      901 ccaaatgaga tatggcagtc agctataaaa taatggggat tcgaaacgcc tgaagatcat
      961 agcaatattc acctattgag gcgtttcgac cgggctgtca gctttcgtca attgattgcc
     1021 ttcgcaaatc aattgacgcc gcctccatcc ctatcccatc gtcagtccgg catagcagtt
     1081 cctactttaa atcagcagat gacctattcc gatttttcgg aaaattcttt gattttctgc
     1141 tttacttcgg ctgatgcatt tggatgaacc aaaatgtgag gatacaagta ttcctgaaac
     1201 tctgcccatt ctcgcgggag cttaaagtcc tttgcggtct ttgaggccag atactctaaa
     1261 gtatcctttt ccatattcgg attggaacac agatttgaaa gcagccccca gtcagttaag
     1321 gacatctttt ttatcagccg atcaatagat tcagccgaaa gattcgtatg ccttgcagca
     1381 gaataattac gctgccttac ctgatcttcc agaatcgatg taggaactgc tggatttttc
     1441 aggatttccg tttctgcgaa tgatctaatc aaagagtagg aactcttata tttggcatat
     1501 ttttcctcga gatccttttc gctgagggaa ggatttgaga gggatgcgat tgaacgggca
     1561 acccagataa aaatcactgt cgcggtaaaa acaccgtaag agtacagaga tgaaggagaa
     1621 agtgctgtat
//
LOCUS       DAOQGJ010001061         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55610_length_1630_cov_2.713016, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001061 DAOQGJ010000000
VERSION     DAOQGJ010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55610_length_1630_cov_2.713016"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PK683_15060"
     CDS             complement(<1..690)
                     /locus_tag="PK683_15060"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNH09814.1"
                     /translation="MNRHSQLKIRIFADGANKKEILELNQNPFVKGFTTNPSLMKKAG
                     ITDYLSFSREVLSEIKDKPFSLEVFADDLSEMERQAKIIASLGENVYVKIPVMNTKKE
                     STAQLVSSLSSAGIKINITAVFTVQQSLEMVSALKNDTPAFLSVFAGRLADTGIDPVP
                     LVAGSVGAASMKKNVEVLWASTREVWNIYQAEALGCGCITAPGDVIKKLNQDYKSAYD
                     QSHETVLTFFRD"
     gene            complement(749..>1630)
                     /gene="mnmA"
                     /locus_tag="PK683_15065"
     CDS             complement(749..>1630)
                     /gene="mnmA"
                     /locus_tag="PK683_15065"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017854419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNH09815.1"
                     /translation="VIKMEKLFQEKVIDRFINDYKEGKTPNPCVECNTFVKFGALFEK
                     AKALGVSKIATGHYARIVEKNGRYAVANGKDRKKNQAYYLYGLSQDNLMNTVFPLGEM
                     EKPEVREHARRFGLKVAEKAESQEICFIPENDYRSFLKKKNVEFTPGFFKLSGGQIIG
                     KHSGKEGFTVGQRKGLGISWKKPLYVLSIEDDGTVYLGEEGETESAEFEVEDVNFQGL
                     APLENGSSFRCRVQIRYRHTPVPCNVSLIDGKYKVRLLQPAASITPGQSAVFYHETED
                     WLLMGGIICKNKFIQRV"
BASE COUNT          397 a          369 c          325 g          539 t
ORIGIN      
        1 atccctgaaa aatgtaagaa ctgtttcatg agactgatca tatgctgact tatagtcttg
       61 attcagtttt ttgatgacat ctcccggtgc tgtaatgcac ccgcacccca gtgcctcagc
      121 ctgatagatg ttccagacct ctctggtcga tgcccagagc acttctacat tttttttcat
      181 tgaggcagcc cctacagagc cggcaacaag aggcacggga tcgatgcctg tgtctgccag
      241 tcttcctgca aaaacagaca ggaacgcagg agtgtcattt ttgagagcag ataccatttc
      301 cagactctgc tgaacagtaa aaactgctgt gatattgatc ttgattcctg cagaagaaag
      361 actggagacg agctgggctg tggattcctt ttttgtattc atcaccggaa tttttacata
      421 tacattttct ccaagcgagg ctatgatctt tgcctgtctt tccatttcac tcagatcatc
      481 tgcaaaaact tccagagaaa aaggtttgtc ctttatctcg gacagcactt ccctcgaaaa
      541 gctgagataa tcagtgattc ctgctttttt catcagagag ggatttgttg tgaatccttt
      601 cacgaacgga ttctgattga gttcaaggat ttctttttta tttgctccat ccgcaaatat
      661 tctgatttta agctgagagt gtctgttcat ttgctgtcct ggttatgtaa ttataaaatc
      721 gaaaaaagag tccggataat tgaggaattt agactcgctg aataaattta tttttgcaga
      781 tgattccgcc catcagaagc cagtcttcgg tttcatggta aaaaacggct gactgtccgg
      841 gggtaatgct tgccgcaggc tgaaggagtc tgactttgta cttaccgtca atcagactga
      901 cgttgcatgg aaccggagtg tgtctgtatc ggatctgaac cctgcatctg aaagatgaac
      961 cgttttcgag cggtgccagt ccctgaaaat tgacatcttc cacttcaaac tctgctgatt
     1021 cagtttctcc ttcctcgcct aagtagacag tgccatcatc ttctatggaa agtacgtaaa
     1081 gaggtttttt ccaggaaatt cccagtcctt ttctctgacc tactgtaaat ccttccttgc
     1141 ctgaatgttt tcctatgatc tgtccgcctg aaagtttgaa aaagccggga gtaaattcca
     1201 catttttctt tttcagaaaa cttctgtagt cgttttccgg aatgaagcag atttcctgag
     1261 attctgcctt ttcggcaact ttcagtccga aacgtcttgc atgttctctt acttcgggct
     1321 tttccatttc tcccagcgga aaaactgtgt tcatcaggtt gtcctgagac agaccgtaaa
     1381 ggtaatatgc ctgatttttt ttcctgtctt ttccgtttgc cacagcatac cgtccgtttt
     1441 tctcaacaat tctggcataa tgacctgtag ctattttaga tactcctaat gcttttgcct
     1501 tttcaaacag tgctccaaat tttacaaagg tattgcattc tacgcatgga ttcggcgttt
     1561 tcccttcttt gtagtcattg atgaatctgt ctatgacttt ttcctgaaag agtttttcca
     1621 tcttgatcac
//
LOCUS       DAOQGJ010001062         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55648_length_1629_cov_3.629606, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001062 DAOQGJ010000000
VERSION     DAOQGJ010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55648_length_1629_cov_3.629606"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1629)
                     /locus_tag="PK683_15070"
     CDS             complement(<1..>1629)
                     /locus_tag="PK683_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH09816.1"
                     /translation="AAIENVNALVADAGMLSKAAVEGKLATRADASKHQGDYRKIVEG
                     VNQTLDAVIGPLNVAADYVDKISKGAIPPKITDSYNGDFNTIKNNLNAAIENVNALVA
                     DAGMLSAAAVEGKLATRADISKHQGDYRKIVEGVNKTLDAVIGPLNVAADYVDRISRG
                     AIPSKITDSYNGDFNTLKNNLNRCIGNINAMVNDADMLAQAAVEGRLSTRADASRHEG
                     DFKKIVDGVNQTLDAVIEPIQETASVLYEMAKGNLQKNVIGKYNGDHAQLADALNTTL
                     AMIREKISEISYVLNAVGKGDLTVTLQSEYVGDYIEIKTSLSAIIDSMKQIVGDLKGV
                     TAQVSNSSEQLDALSQSLSQGANMQAANLEETSASIEELLASVMQNAQNAKATDQIAN
                     VTSKQAVEGGKAVQETLRAMKEIAEKINVVEEIASQTNLLAVNASIESARAGEHGLGF
                     AVVAGEVRKLAEGSKHAAKEIKELAGKSLNVAENADRLLNEIVPSIAKTANLVQEIAA
                     ASEEQSDNLKNFNTAISQLNNVSQQNAASSEELAA"
BASE COUNT          362 a          396 c          335 g          536 t
ORIGIN      
        1 tgccgccagt tcttcggaag aagctgcatt ctgctgagat acattgttca gctgactgat
       61 tgcagtattg aaatttttca ggttgtcact ctgttcttcc gatgcggctg caatttcctg
      121 aacaaggttt gccgttttcg cgatgcttgg gacaatttca ttcaaaagtc tgtcagcatt
      181 ctctgcaacg tttaaacttt tgcctgcaag ttccttgatt tctttcgcag catgtttgct
      241 tccttctgcg agcttcctga cctcacctgc aacgacagca aatccaagac cgtgttctcc
      301 tgctcttgcc gattctatgg atgcattgac tgcaagaaga tttgtctgag aggcgatttc
      361 ttccactaca ttgatttttt ctgcaatttc tttcatcgct ctcagtgttt cctgaacagc
      421 ctttccgccc tcaacggcct gtttggaagt cacatttgca atctgatccg ttgcctttgc
      481 attctgagca ttctgcatga cggaagccag aagttcttcg atagatgcgc ttgtctcttc
      541 aagattggcg gcctgcatat ttgcaccctg actgagcgac tgagaaagtg catcgagctg
      601 ttccgaggaa ttggaaacct gtgctgtaac tcctttcaag tcgccaacaa tctgtttcat
      661 ggaatcaatg attgcagaaa gagaagtttt tatctcaata tagtctccca catattcaga
      721 ctgaagagtg acagtcagat ctcccttgcc gactgcattc aatacgtatg aaatctcact
      781 tattttctct ctgatcattg ccaaagttgt attgagtgca tctgccaatt gtgcatgatc
      841 gccattgtat tttccgatta catttttctg cagatttccc tttgccattt cataaagaac
      901 ggaagctgtt tcctgaatcg gttcaatgac tgcatcgaga gtctgattga ctccatcgac
      961 aatctttttg aaatcacctt catgcctgga tgcatctgct cttgtagaca gtcttccttc
     1021 cactgctgcc tgagcaagca tatccgcatc gttgaccatc gcgttgatgt ttccgatgca
     1081 tcggttgaga ttgtttttca gagtgttgaa atccccgtta tatgaatctg tgatcttcga
     1141 aggaattgca cctctggata ttctgtctac gtaatccgct gcaacattga gcggcccgat
     1201 gactgcatcg agagtcttat tgacgccttc cacaatttta cggtagtctc cctgatgttt
     1261 tgaaatatct gctcttgtgg ccagttttcc ttccactgct gcggcagaaa gcattcctgc
     1321 atcagctaca agtgcattca cattttcaat ggctgcattg agattgttct tgattgtatt
     1381 gaaatcaccg ttataggaat ctgtgatctt cggaggaata gcacccttag agattttatc
     1441 cacgtagtca gcagctacat tgagaggccc gatgactgca tcgagagtct gattgactcc
     1501 ttccactatt ttgcggtagt ctccctgatg ttttgaagca tctgcccttg tggccagttt
     1561 gccttccact gccgcttttg aaagcattcc tgcatcagct acaagtgcat tcacattttc
     1621 gatagcggc
//
LOCUS       DAOQGJ010001063         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55893_length_1625_cov_3.822293, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001063 DAOQGJ010000000
VERSION     DAOQGJ010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55893_length_1625_cov_3.822293"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..>1625)
                     /locus_tag="PK683_15075"
     CDS             complement(18..>1625)
                     /locus_tag="PK683_15075"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HNH09817.1"
                     /translation="GSLSDKKNISRHDRVTFRKYEMQQDFLFPPSTEDYLPPNHIARL
                     VSAIVDRMDLSPIVSKYKGGGVSAYHPGMMLKVWLLGFIKKIYTSRKLEKALTENIAF
                     MWISGRQNPDHKTLSNFRAMLADDIKVIFRNIVKMGLETGIIEGKDVFMDHTKIQADA
                     NPYKMLWKKSVKKRTEAVDAELDMLFEHIQKLNEKEDADYGNSSLDNIKKEVFSEENL
                     DSVAERINSDLNDGIITKEDASGAKKKLKRAGELLKRKERLAKQEEILDGRSGCSTTD
                     SDASAMKMKRSDEIRPGYNEGVVTENGFITAFDVSRNPADSVSFISLAEEAETNLGKK
                     PESLTADAGYGSLENYEYLKEKKIENYVQYPGWFREGKFQGKFSIRDFQYDPADNSFI
                     CMNSETLTFTGTKQKTTATGFKEEISVYSAPKSACALCPKKAYCTEYENKNLHVNWKL
                     EEHKKAVRENLRTEKGTALRKKRSHDVETVFGDRKWNQGRRRYLLRSLPKVTVEAGLH
                     SIAHNLKKMFFYIHSVLYPRRDFREVI"
BASE COUNT          352 a          417 c          309 g          547 t
ORIGIN      
        1 aaatttttcg ttttttttta tatgacttca cggaagtccc gcctcggata gagaaccgaa
       61 tgaatataga agaacatttt cttgagattg tgtgcgatgg agtgaagacc ggcttcaacg
      121 gtgactttcg gaagagaacg gagaagatac cgtctgcgtc cctgattcca ttttctgtct
      181 ccgaaaacag tttccacatc gtgtgacctt ttctttctga gagcagtgcc tttctccgtt
      241 ctcagatttt ctctcactgc cttcttgtgc tcttcaagtt tccagtttac atgaagattt
      301 ttattttcat attctgtaca ataagctttt ttaggacaaa gtgcgcaggc tgatttcggt
      361 gcggaataga cagaaatctc ttccttgaat ccggtcgcag tcgttttctg ttttgtcccg
      421 gtgaatgtca gagtttcact gttcatgcat atgaatgaat tgtctgcagg atcatactga
      481 aaatcacgga tgctgaactt tccctgaaac tttccttctc tgaaccagcc tggatactga
      541 acatagtttt ctattttctt ttctttcagg tattcgtaat tttcaagcga tccatagcct
      601 gcatctgcag tcaatgattc cggttttttt ccgagatttg tctcggcttc ttccgcaagg
      661 ctgatgaaac tgaccgaatc cgccggattc cggctgacgt caaatgcagt gatgaaaccg
      721 ttttcagtca cgaccccttc attatagcct ggtctgatct cgtcgcttct cttcatcttc
      781 attgcactcg catcactgtc cgtcgtcgag cagccgcttc ttccgtccag aatctcttcc
      841 tgcttcgcaa gcctctcctt ccttttcaga agttctccgg ctctcttcag tttcttcttc
      901 gctccggatg catcttcttt tgtaatgatg ccatcattaa gatctgaatt gatcctttcg
      961 gcgaccgaat ccagattctc ttcagagaac acttccttct ttatgttgtc cagactgctg
     1021 tttccgtaat ctgcatcttc cttctcattc agcttctgta tgtgttcaaa cagcatatcc
     1081 agctcggcat cgactgcctc tgttctcttc ttcacgcttt ttttccagag catcttatac
     1141 ggatttgcat ctgcctgaat cttggtatga tccatgaata catcctttcc ttcgatgatc
     1201 cctgtctcaa gacccatctt cacaatgttt ctgaatatca ctttgatgtc gtccgcaagc
     1261 attgcccgaa aattcgaaag agtcttatga tccggattct gtctgccgct tatccacata
     1321 aatgctatgt tctcagtcag cgccttttcc agtttccggc tggtatatat tttcttgatg
     1381 aaacccagaa gccatacctt cagcatcatt ccaggatgat aggcgctcac tccgcctccc
     1441 ttgtatttgg agacaatcgg actcagatcc attctgtcca ctatcgcact cacaagacgg
     1501 gcaatgtgat tcggaggaag ataatcttcc gtgcttggcg gaaacagaaa atcctgctgc
     1561 atctcatatt tcctgaatgt caccctgtcg tggcgggaaa tgttcttttt atctgacagc
     1621 gaccc
//
LOCUS       DAOQGJ010001064         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55910_length_1625_cov_2.854777, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001064 DAOQGJ010000000
VERSION     DAOQGJ010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55910_length_1625_cov_2.854777"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PK683_15080"
     CDS             complement(<1..185)
                     /locus_tag="PK683_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09818.1"
                     /translation="MNYIYIVFIFILSFCGGEKSGRLFPSFMILESEHSPGKYISGEP
                     GKLGFSDYRHNHISYSR"
     gene            complement(182..1306)
                     /locus_tag="PK683_15085"
     CDS             complement(182..1306)
                     /locus_tag="PK683_15085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH09819.1"
                     /translation="MELFIDKEKLKQIKESRSSEKTHTILIVDDERENAESLAMILED
                     NYEIIKTGSADQALDYIQTDPDPGKISLIISDQRMPGMTGVEFLRETIFIIPNAIRIL
                     ITGFADIKDIIDSINIGQIYKYITKPVEPKELLVTVKRALEAYDMNQKNRELIMELKR
                     VNESLERIVEERTRQLQESLEELRKQKQELEYFTEFQDKLVNELQELSLTDPLTGISN
                     RRTMEAFAETEFGRAARNRTQISFMMIDIDHFKLYNDHYGHAMGDKCIKNIAGILTDS
                     LKRSADSAARYGGEEFCCILPETDTVGALKVAEEIISRLCSLRIVHEKSLTSPYVTVS
                     IGIFSVIPGPGDFWKDALSKADERLYLAKKNGRNRIEYSK"
     gene            complement(1314..1571)
                     /locus_tag="PK683_15090"
                     /pseudo
     CDS             complement(1314..1571)
                     /locus_tag="PK683_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
BASE COUNT          422 a          383 c          289 g          531 t
ORIGIN      
        1 ttccgtgaat aactgatatg attatggcga tagtccgaaa aaccgagttt tccaggttcg
       61 cctgaaatgt atttcccggg agaatgttcc gattccagga tcataaatga agggaaaagt
      121 cttccggatt tttctcctcc gcagaaactt aaaataaaaa taaatacaat ataaatataa
      181 ttcatttact gtattctatt ctgttgcgtc cgtttttttt agccagatag agacgttcat
      241 ctgctttgga aagagcatcc ttccaaaaat cccccggtcc gggaatcacg gaaaaaatcc
      301 cgatgctcac agtgacatac ggagatgtta aagatttttc atgaactatc cggagcgaac
      361 acagtctgga aatgatttct tcagccacct tcagtgcacc gactgtatct gtctcaggaa
      421 gtatgcagca gaattcctcg cctccgtacc gggcagccga atcagctgat cttttcagag
      481 agtctgtcag tattcctgct atatttttaa tgcatttatc tcccattgca tgtccataat
      541 gatcattata cagcttgaaa tgatctatgt ctatcatcat aaatgaaatc tgtgttctgt
      601 tccttgcagc tcttccaaat tctgtttcag caaaggcctc catcgttctt ctgttggaaa
      661 ttccagtcag cggatcagtc agtgaaagtt cctgaagctc atttacaagc ttgtcctgaa
      721 attccgtaaa gtactccagt tcctgtttct gttttctgag ttcttcgagt gattcctgaa
      781 gctggcgggt cctctcctcc acaattcttt ccaggctttc gttcactctc tttaattcca
      841 tgatcagctc tctgtttttc tgattcatat cataagcttc gagagccctc ttgacagtca
      901 caagcagttc cttcggctca accggtttgg ttatatattt atatatctgt ccaatattta
      961 tagaatcgat aatatcttta atatcggcga aaccggtaat cagtattctt attgcgttcg
     1021 gaattataaa tatcgtttcc cgcagaaatt ccacccctgt cattccaggc attctctgat
     1081 cagaaataat caggctgatt ttccccggat cagggtcagt ctgaatatag tcaagtgcct
     1141 gatccgcact tcctgtttta attatttcat aattatcttc aaggatcatc gcaagagact
     1201 cggcattttc tctctcatcg tccacgatga gtatggtatg agttttttcc gaagatcttg
     1261 attccttaat ctgcttcagt ttttccttgt cgatgaaaag ttccatagcc tgcctactga
     1321 agctctgaat acagttcaaa gaactgactg ttgggcagaa ttccctgagt gtccctggaa
     1381 agttctcttc tgattccgtg aacaaaatac atatctacat ctcccttatt cttcacactt
     1441 atccttcccc tgtattcagt atcaaagaaa tttttgacag ctgaatatgc tgctccgctt
     1501 atatttatca ttcccgcagc gcctcccgat tccatcctgc tggcagtgtt cacagtgtca
     1561 ccccagacat cggcaatgtc ccgaacattg ttgtaaatga gcagatacta agcaacttca
     1621 ggtaa
//
LOCUS       DAOQGJ010001065         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_55971_length_1624_cov_3.038241, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001065 DAOQGJ010000000
VERSION     DAOQGJ010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55971_length_1624_cov_3.038241"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PK683_15095"
     CDS             complement(<1..173)
                     /locus_tag="PK683_15095"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNH09820.1"
                     /translation="MDFYYRVGRYSLYLLSFLLLLVFTRIFLFQIYRISGNSMLPTLK
                     HGDIVLVYKYKFP"
     gene            complement(173..>1624)
                     /locus_tag="PK683_15100"
     CDS             complement(173..>1624)
                     /locus_tag="PK683_15100"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH09821.1"
                     /translation="FNSMVDSIRDARLRLEDYAQNLEHKVQERTKEVQEKMEEVQKLK
                     IQQDGDYFLTSLLAKPLFFNANKSEYVKTEFIVNQKKQFQFRNKIGDLGGDICITGNL
                     KFGIPADHRRYTMVMNGDAMGKSMQGAGGSLVMGVVMNSIMARSASNKRVLDMSPEKW
                     LTDVYNEIHSVFKTFNGTMVISATVALIDDLTGRMLYWNAEHPFSVLYRDMNASFIET
                     GLNLRKLGLESEYEFQIFEFQMEKDDVIILASDGRDDIDLTPHEDVRTINDDEMLFLK
                     IVEEGKGEINDIVHVLKSRGEITDDLSILKVTYIGAPQVQEKTNGHYSAEYLTQKRDL
                     VKKINGLYAEGRDLYYSGRVPAAKEKIQEAYGMSTEFPKLNRLYALLCYKTKDYMTAL
                     NLINGVLQQYPNDAEMWHYLSRTHRKLGSIQIALECAERAERLKPGTVQNILHMARLY
                     RAQGNSEKTKEFAEKVLELDFENKSARKILSML"
BASE COUNT          420 a          387 c          297 g          520 t
ORIGIN      
        1 tgggggaact tgtacttata taccagtaca atatccccgt gtttcaaagt gggaagcatt
       61 gaatttccgc tgattctgta aatctgaaac aggaatattc tggtaaaaac cagcagcaaa
      121 aggaaggaca gaagatataa ggaatatctg ccgactctat agtaaaaatc catcacaaca
      181 ttgacagtat tttacgggct gatttatttt caaaatctaa ttccagtacc ttttcggcaa
      241 attccttcgt cttttccgaa ttcccctgcg ccctgtaaag ccttgccata tgcagtatgt
      301 tctgaacagt tcccggcttc agacgttctg ctctttccgc acattccaga gctatttgaa
      361 tgcttcccag ctttctgtga gttctggaaa gataatgcca catttccgca tcattcggat
      421 actgctgaag cactccattg atgagattga gagccgtcat ataatccttc gtcttgtaac
      481 agagaagtgc atacagtctg ttcagtttgg gaaattctgt ggacattcca taggcttcct
      541 gaatcttttc ctttgcagcg ggcactcgtc ccgaataata gagatccctg ccttctgcat
      601 aaagcccgtt tatttttttg accagatctc ttttctgagt gaggtattcc gcactgtaat
      661 gtccgtttgt tttttcctga acctgaggtg cgcctatata agtgactttc aggatggaaa
      721 gatcatctgt aatttcccct ctggatttca gtacatggac tatgtcattg atctcccctt
      781 ttccttcttc tacgattttc aggaaaagca tttcatcgtc attgattgtt cggacatctt
      841 catgaggagt gagatcaata tcgtcccggc cgtcagaagc aagaatgatc acatcatcct
      901 tctccatctg aaattcaaat atctgaaact cgtattccga ttccagtcca agctttctca
      961 gattcagtcc tgtttcaatg aaacttgcat tcatatcccg gtacagaacc gaaaagggat
     1021 gttcagcatt ccaatacagc attcttccag tcagatcatc aatgagagcg acagtagcgg
     1081 atattaccat tgttccgttg aatgtcttga atacggaatg aatttcattg tacacatcag
     1141 tcagccattt ttcaggactc atatccagca ctcttttgtt ggatgcggaa cgtgccataa
     1201 tggaattcat cacaactccc attacaagag atcctcctgc cccctgcatg gacttgccca
     1261 tcgcatcccc gttcatcacc attgtataac gtctgtgatc tgcaggaatc ccaaatttca
     1321 gatttcctgt aatgcagata tcccctccca gatcgccaat cttgtttcgg aactggaact
     1381 gttttttctg atttacgata aattccgtct ttacatactc agatttattt gcattaaaaa
     1441 ataatggttt tgcaagaagt gaggtcagga aatagtctcc gtcctgctga attttcagct
     1501 tctgaacctc ttccattttt tcctgaactt cttttgttct ctcctgaacc ttatgctcca
     1561 gattctgggc ataatcctcc agcctgagtc tggcatctct gatggaatca accatactgt
     1621 tgaa
//
LOCUS       DAOQGJ010001066         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56020_length_1623_cov_4.251913, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001066 DAOQGJ010000000
VERSION     DAOQGJ010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56020_length_1623_cov_4.251913"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1623)
                     /locus_tag="PK683_15105"
     CDS             complement(<1..>1623)
                     /locus_tag="PK683_15105"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455098.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNH09822.1"
                     /translation="ASADPGTQYHTTINEWHTCPEDGEIKASNPCSEYMFLDNTACNL
                     ASANLQKFLKSDGTFDVHAFRYVCMIWTLILEISVTMAQFPSKEIAELSYQFRTLGLG
                     YANLGSILMLMGIPYDSKEALGMIGAITSIMHMTAYKTSALIAKELGTFPGYAKNKSH
                     MLKVMRNHRRAAYNSSPDEYEGLTVKPMGIDSANCPAYMLQAAREDADEALRLGEEFG
                     YRNAQVTVIAPTGTIGLVMDCDTTGIEPDFALVKYKKLAGGGYFKIINQSVPAALKKL
                     GYSASEIEAIVNYCKGYATLNGAPHINPQSLKEKGFTDELLQKIEKELPTAFDISFAF
                     NKFVLGEDFLSKTLKIPKETYDSFSFSLLEYLGFTKEEIRKCGDYVCGTMTVENAPFL
                     KEKDLPVFDCANKCGRYGKRYLSYESHIRAMAAAQPFISGAISKTINLPEEATVDEVK
                     NAYLLSWKLMIKANALYRDGSKLSQPLNSVLDLISSEETPEVSIAEPTNSQMKAVEKI
                     VYKYISNRRKLPSRRAGYTQKASVGGHKVYLRT"
BASE COUNT          409 a          373 c          360 g          481 t
ORIGIN      
        1 gtgcggagat aaaccttgtg cccgccgact gacgctttct gagtataacc ggctcttctg
       61 gaaggaagtt ttcttctgtt ggagatgtat ttatatacaa ttttctccac ggccttcatc
      121 tgagaatttg tcggctcagc aatgctcact tcaggggttt cttccgaact gatcagatcc
      181 aaaacagagt tcagcggctg ggaaagtttt gaaccgtctc tgtaaagagc atttgccttg
      241 atcatcagtt tccaggaaag aagatatgca ttctttactt catccacagt ggcttcttcc
      301 ggaagattga ttgtcttgga aatggcaccg cttatgaaag gctgagccgc cgccattgca
      361 cggatatgag attcatagga aagatatctc ttgccgtaac ggccgcattt attcgcacag
      421 tcaaataccg gcagatcttt ttccttcaga aatggagcat tttccactgt cattgttccg
      481 cagacgtagt cgccgcattt gcggatctcc tccttggtga atcccagata ttcaagaaga
      541 ctgaatgaaa atgaatcgta agtttcttta ggaattttca gagtttttga aaggaagtct
      601 tccccaagta caaacttgtt gaatgcaaag cttatatcga atgctgttgg gagctccttt
      661 tcgatcttct gcagaagttc atcagtaaat cctttttctt tcagagactg aggattgata
      721 tggggtgctc cgttcagtgt agcataccct ttgcagtagt ttacgattgc ttcaatttcg
      781 gatgcactgt atcccagttt tttcagagcg gctggaactg actggttgat gatcttgaaa
      841 taaccccctc ctgccagttt cttgtatttc acaagagcaa agtccggctc aattccggtc
      901 gtatcacagt ccatgacaag accgatggta ccggtaggag caatcacagt gacctgtgca
      961 tttctgtatc cgaattcctc accgagtctc agtgcctcat ccgcatcttc acgggcagcc
     1021 tgaagcatat aagcaggaca gtttgcagag tcaattccca tcggcttgac agtaaggcct
     1081 tcatactcgt caggactcga attgtatgcg gctcttctgt gatttctcat caccttcagc
     1141 atatgggatt tgttctttgc ataaccggga aatgttccga gctcctttgc aatcaaagcc
     1201 gatgtcttgt acgcagtcat atgcataatc gaagtaattg caccgatcat tcccaatgct
     1261 tctttggaat cgtaaggaat tcccatcagc atcagaatgg atccgagatt tgcatacccc
     1321 agtccaagag ttctgaactg ataggacagt tctgcaattt ctttagaagg gaactgagcc
     1381 attgtcacag aaatttccag gatcagggtc cagatcatac acacgtatct gaatgcatga
     1441 acatcaaatg taccgtctga tttcaggaat ttctggaggt ttgcagaagc cagattgcag
     1501 gcagtattgt ccagaaacat atattccgaa caggggttcg aagccttgat ctcgccgtcc
     1561 tcaggacagg tatgccattc attgattgtt gtatgatact gtgtacctgg gtctgcactc
     1621 gcc
//
LOCUS       DAOQGJ010001067         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56051_length_1623_cov_2.526786, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001067 DAOQGJ010000000
VERSION     DAOQGJ010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56051_length_1623_cov_2.526786"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PK683_15110"
     CDS             complement(<1..386)
                     /locus_tag="PK683_15110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNH09823.1"
                     /translation="MNIEILHRPSNTAAKVTLGPNETITSEAGAMIAMSGDLSITTST
                     HQKNSGGGFLKALKRMFAGESFFLNHFEAGPKGGELYLATSLQGDMQVFEGKGESLII
                     QSGAFVACEQSISIEPGWQGFKNLFSG"
     gene            complement(395..1057)
                     /locus_tag="PK683_15115"
     CDS             complement(395..1057)
                     /locus_tag="PK683_15115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNH09824.1"
                     /translation="MKTEINGAPAFAYVHVDLEPGESFIAESDAMSSMSTELELRPKF
                     NGGFFKGLLRKFLGGESLFINEFSNPSSSTQRLTIVQPTPGDIREIELKDNTICMQPG
                     AYIASTPGVKIGLQWAGFASFIAREGLFKITASGTGKLLYGCYGGLVEKEVKGEYIVD
                     SGHLVAYEPQLQLKLKLAGGLISSFTSGEGIVTKIIGNGKIYLQSRSLEGLASWINPH
                     LY"
     gene            complement(1083..>1623)
                     /locus_tag="PK683_15120"
     CDS             complement(1083..>1623)
                     /locus_tag="PK683_15120"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNH09825.1"
                     /translation="MEDSRVNAFAIPGGNIVIHSELIMKAERPEEIAGVLAHELSHIT
                     EKHGLRQIINSVGLFVVIQTLFGDFTGLLAIITQNSGLLISSKFSRDYEREADAQGFK
                     YLLTAKIHPSGMKEFFEKIQKLESEDKTEGEDVLRFLSTHPGTEERIETMKEKLSHLN
                     PKDFADIKMELAKLKAKLK"
BASE COUNT          387 a          404 c          331 g          501 t
ORIGIN      
        1 ccggaaaaca gattcttgaa tccctgccag cccggctcaa tcgaaatgct ctgctcgcag
       61 gctacaaatg cgcctgactg aatgatgagg ctttcgccct tcccttcaaa aacctgcata
      121 tctccctgaa gagaagttgc aagatacagc tctcctcctt tcggtcctgc ctcaaaatgg
      181 ttcaggaaaa aagactctcc tgcaaacatt cttttcagag ccttcagaaa tcctcctcct
      241 gaattcttct gatgagtgga agtagtaatg ctgagatcgc ctgacattgc aatcattgct
      301 cccgcttccg acgtgattgt ttcattcggc cccagagtca ccttggctgc cgtattgctt
      361 ggtctgtgta atatctctat attcattttc cctctcagta cagatgcgga ttgatccagg
      421 acgcaagtcc ttccagactt ctcgactgca gatatatctt tccgttcccg atgattttag
      481 tcacaattcc ttcaccgctg gtgaagctcg aaatgagtcc gcctgccagt ttcagtttga
      541 gctggagctg cggttcataa gccacgagat gcccggaatc tacaatgtat tcgcccttga
      601 cttctttttc cacgagtccg ccgtaacatc cgtacagaag ttttcccgtt ccagaagcag
      661 tgatcttgaa aagaccttct cttgcgatga aggaagcaaa acctgcccac tgaagtccaa
      721 ttttgactcc aggagtgctt gcaatataag ctcctggctg catgcagata gtattatcct
      781 tcagctcaat ttcccggata tcgcctggag tcggctgcac aattgtaagt ctctgagtcg
      841 aactggaagg attgctgaat tcattaataa ataatgattc tcctcccagg aattttctga
      901 ggaggccctt gaaaaaacct ccgttgaatt ttggccgcag ttccagctct gtggacatgc
      961 tggacattgc atcggattcg gctataaagc tttcaccggg ttccaaatct acatgcacat
     1021 aggcaaatgc cggtgcgccg ttgatttctg tcttcataat ttaccctctt aatttacgtc
     1081 gtctatttca gtttcgcttt aagttttgcc aattccattt ttatgtctgc aaagtctttg
     1141 ggattcagat gagaaagttt ttctttcatc gtttcaattc tctcttctgt tccgggatgt
     1201 gtgcttaaaa aacggagcac atcttctcct tccgtcttgt cctcactttc gagtttctgt
     1261 atcttttcaa aaaattcctt cattccggaa ggatgaattt ttgcagtgag aagatacttg
     1321 aacccctgag catctgcttc tctttcatag tctctcgaaa acttggaaga gatgagaagt
     1381 ccgctgttct gtgtgatgat tgcaagaagt cctgtgaagt ctccgaacag tgtctggatg
     1441 actacaaaaa gtccgacgga attgatgatc tgacgcagtc cgtgtttttc agtgatatgt
     1501 gaaagttcat gtgccagaac tcctgcaatt tcctcgggtc tttccgcctt cataatcagt
     1561 tccgaatgaa ttacaatatt tcctcccggt atggcaaatg cattgactcg cgagtcttcc
     1621 ata
//
LOCUS       DAOQGJ010001068         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56238_length_1620_cov_2.496486, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001068 DAOQGJ010000000
VERSION     DAOQGJ010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56238_length_1620_cov_2.496486"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1065
                     /locus_tag="PK683_15125"
     CDS             106..1065
                     /locus_tag="PK683_15125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH09826.1"
                     /translation="MLVSIVIPTYNQGPYIRDCLESVTGQTYKNLEIIIQDSCSSDET
                     EKICLEFAEKDKRVRYFRETDKGQSDAINRGLARSSGYWWNWICSDDRYNSFSVIEKL
                     VYEFEQKYMNQKDFIGIYGNACFITEQGSYISDYHNKKKILGREDFQLTWPLSQPSCL
                     LKRAAVADVGGVNADLYLGMDLDLFFKIMKKQEKVSFLDYLICDIRIQDNSKSIKYRK
                     DTAKNALDLVRFHMKSTGPLHQSDYFKEYLFAAESDEINEYLTTIPFLDKWRILYELS
                     PLGNLKGKLKAQILKLASKNRLFHRLLHFTYNSWIVKKILGKR"
     gene            1099..>1620
                     /locus_tag="PK683_15130"
     CDS             1099..>1620
                     /locus_tag="PK683_15130"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNH09827.1"
                     /translation="MKNFSIIGVGGYIAPRHLKAVKETGNHLLSAYDRNDSVGIMDSH
                     FPDADFFTEFERFASFNDRQKRAGKSIDYVSIMTPNYLHFSHMEYGLSIGADVICEKP
                     LVLHPEDIDELMVLEKKYGKRVSTILQLRHHQVIIDLKEKIDKLPKDKKIEIDLTYIT
                     SRGKWYFESWKGDL"
BASE COUNT          548 a          256 c          349 g          467 t
ORIGIN      
        1 cggagttcag acagatcgtg aatctgtaaa gaccgttgag atgaaatatt taatcattaa
       61 aacttactgc agtgatcagt ttctaagtca ggaggtgttc ttcctttgtt agtcagtata
      121 gtcattccta catataatca aggtccctat atcagagatt gcctggaatc agtcacaggg
      181 cagacttata aaaatctcga aattattatt caagattcct gttcgagcga tgagactgaa
      241 aaaatctgtc tggagtttgc agaaaaggat aaacgagtaa gatatttcag ggaaacggat
      301 aaaggacagt ccgatgcgat taatcgcggt ttggcaaggt cttccggtta ttggtggaac
      361 tggatatgct cagatgacag atataatagc ttttctgtta tagaaaaact ggtctatgaa
      421 tttgagcaga agtatatgaa tcagaaagat tttataggaa tatacgggaa tgcctgtttt
      481 ataactgaac aaggcagtta tatttctgat taccataata aaaagaaaat tttaggaagg
      541 gaagattttc aactcacttg gcctctctcg cagccttcct gtcttttgaa aagagctgct
      601 gtcgccgatg ttggcggagt aaatgcagat ctatatctgg gtatggatct ggatcttttt
      661 tttaaaataa tgaaaaagca agaaaaagta agttttcttg actatctgat ttgtgatatt
      721 cgaattcagg ataactcaaa aagtataaaa tacagaaaag atacggccaa aaatgcctta
      781 gatctggtga ggtttcatat gaaaagcact ggacctttgc atcagtctga ttactttaag
      841 gaatatttat ttgctgcaga atctgacgag attaatgagt atctcacaac gattcctttt
      901 ttggacaagt ggagaatact gtatgaattg tcacccttgg gaaacctgaa agggaagctg
      961 aaagctcaaa tactgaagct agcatcaaag aacagacttt ttcacagatt gttgcatttc
     1021 acatataaca gttggattgt aaagaagatt ctcggaaaga gatgaactgt attaaataaa
     1081 aattatcagg agaataactt gaaaaatttt tccattatcg gtgtgggcgg ttatattgcc
     1141 ccaagacatt tgaaagccgt aaaagagaca ggaaatcatc tgttgtctgc gtatgaccga
     1201 aatgattcgg tagggatcat ggacagtcat tttcccgatg cagatttttt tacagaattt
     1261 gaaaggtttg ccagcttcaa tgacagacag aagcgtgcag gaaaaagtat tgactatgta
     1321 agcatcatga ctccgaacta tctgcatttt tctcatatgg aatacggact cagcatcgga
     1381 gcagatgtga tctgtgaaaa acccttagtg cttcatccgg aagacatcga tgaacttatg
     1441 gttctggaaa aaaaatacgg aaaaagagtg agcacaatac tgcagctccg tcatcaccag
     1501 gtgatcattg atctgaagga aaaaattgat aaacttccaa aggataagaa aatagaaata
     1561 gatctcactt acatcacctc cagaggaaaa tggtattttg agtcatggaa gggagatttg
//
LOCUS       DAOQGJ010001069         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56267_length_1619_cov_3.778133, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001069 DAOQGJ010000000
VERSION     DAOQGJ010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56267_length_1619_cov_3.778133"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            641..1483
                     /locus_tag="PK683_15135"
     CDS             641..1483
                     /locus_tag="PK683_15135"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNH09828.1"
                     /translation="MPKIDIQIPEEYLFETEITVRKMDIWGDLHVGSHTMFELVMECY
                     FRFWDHCGFTPLDIFGSGLVFSNFSMIFQSEVHYGDTLKTQVSVGNFSDYGCDMFFRM
                     LGREDGREIAQARIYIVFFDFKNKKKEAVGRAVQYLSENMPRMKNINFQNENQPTEEE
                     NKLYRKVMSVAEEVHRFMPANTPESVYRKLFCYELNKVGIGYEVRKKETNTRYKELNV
                     KGIRTEIIIEKQIMVEIKYVEQLNKPIKSKFKSYMKSAGLRLGLLINFNVADLPDGIA
                     RIVL"
BASE COUNT          526 a          297 c          339 g          457 t
ORIGIN      
        1 tttattcata tgatttgcat ctgaaaaatc agataaactc tccttgaatc tatgatcgct
       61 cagctcagta tctgcagtgc tgatgaaaaa tcaagacatt actgcagcta ggcagaattc
      121 catcactgaa gaactcggct gtaattttgt ttttcggacc gaatataatt ttgactgtac
      181 ctccaatatc attgcagctc atccctgccc atataatttt gaactgcagt caaaatccaa
      241 tttcgttttg caatctgata ggaaccggag tcgtaagagc cttccaaagt ttccaattta
      301 ctttccgatg ccagatttat ggaaacagta aaaaaagatt atcctattca ctgttcatct
      361 cctgctaaaa ttgattgact tgtaccgtac gattatggga tacaaaaagc ttttccggag
      421 caagactatt tagtgaaaaa tggggaagat tgtcttttgt caatggattt ttgcattatt
      481 acaatggtcg aagcatcagc agttccagta aaaaaggggg gatttccaaa gtgcagacct
      541 tccagacagt attttgaagt aaagccagga cagctaaatc gaattgactg aattttagat
      601 tagacaaaac tatccttaaa gtccctgaaa taaggccatc atgccaaaga tagatataca
      661 gattccggaa gaatacctct tcgaaacaga aattacagtc agaaaaatgg acatttgggg
      721 agatcttcac gtaggatctc atacgatgtt tgaactcgtc atggaatgtt atttccgctt
      781 ctgggatcac tgcggattta ctcctctgga tattttcgga agcggtcttg tattttccaa
      841 tttcagtatg atatttcagt cagaagttca ctatggagac actctgaaaa ctcaggtctc
      901 agtcggcaat ttcagtgatt acggatgcga tatgttcttt cggatgcttg gcagagaaga
      961 cgggcgggaa attgctcagg cgaggattta catagttttc tttgacttca agaacaagaa
     1021 gaaggaagcg gtgggcagag cggttcagta tctttctgaa aatatgccca ggatgaaaaa
     1081 catcaatttt caaaatgaaa atcagccgac agaagaagag aataaacttt acagaaaagt
     1141 gatgagcgtt gcagaggaag tgcatcggtt tatgcctgca aacactccgg agtctgtgta
     1201 cagaaaactt ttctgctatg aactgaacaa ggtcggcatc gggtacgagg tcagaaagaa
     1261 agagacgaat accagataca aggaactcaa tgtaaagggg atcagaacgg agatcattat
     1321 tgaaaagcag atcatggtgg aaattaaata tgtggaacag ttgaataaac cgatcaaaag
     1381 caagttcaag tcatatatga aatctgccgg gctccgcctg ggacttctga tcaatttcaa
     1441 cgtagctgat cttccggacg gaatcgctag aatagtactg taatttatta tgaaaaattt
     1501 gaatatattt ttgattattt ttctttctgc ctgtatttca gagcagaaac cgcctacagc
     1561 ggttttgaat cctaaaaaac ctcctgttgt caaaaaagat ccagtaaagg aaaaacccg
//
LOCUS       DAOQGJ010001070         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56356_length_1618_cov_2.449136, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001070 DAOQGJ010000000
VERSION     DAOQGJ010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56356_length_1618_cov_2.449136"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..552)
                     /locus_tag="PK683_15140"
     CDS             complement(214..552)
                     /locus_tag="PK683_15140"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNH09829.1"
                     /translation="MNTEKGKWGEKEALNFLVGKGHQVLERNYRNKLGELDIVTLYEG
                     MIYCIEVKSWRNSAVHPLEVMTERKIAKMKLIAKSFFYARKIPEENYLVNICLLYVTG
                     DGIEFFTDLL"
     gene            complement(667..>1618)
                     /locus_tag="PK683_15145"
     CDS             complement(667..>1618)
                     /locus_tag="PK683_15145"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNH09830.1"
                     /translation="DPATAALRNSFHILQKNKDEFVRLYKDSFDAVQTLYRKVAEDKV
                     IELNQIRSIAEQITDHVRANPNFSFMLLNYSLDGYYLYNQVVKATFYSLLIAMSMDYS
                     RPKMVDLAMAGLLSDVGMSKIPGYISEKTATLNEEEYKVVKKHPLFGYQILTKNYKVK
                     NSIALVALQHHENFDGTGYPQKMKKNEIEEASCIYSIADTFSALVSNRPWRRKFQPYE
                     ALKTMISVVMNRFDLNLLRMFLNKIAMYPVGSYVELSDGSIAQVLEANFSKMLRPGLI
                     IVKEAGGEITKKERFVNLLNDNTVYISKAIDGNLIGQISK"
BASE COUNT          460 a          343 c          289 g          526 t
ORIGIN      
        1 tctattttct ttttgtaagc agcaaaaacc ggttcagcag tgtttttctg tgagcctctt
       61 tttctgatca tgatcggttc cggtctgata tttctttcag agatagaatc tataatgcta
      121 agatttgcca taagctgttc ccgaaatctc tgtctccaac aatttttttt atatcttctg
      181 aaaaaggtcc ttaaaaaaaa atctgctcag ctctcagaga agatcggtaa aaaactctat
      241 tccatcaccg gtcacataca agagacaaat attcaccaga tagttttctt ccggaatttt
      301 tctggcatag aaaaatgact ttgctatgag cttcattttg gcgattttcc tttctgtcat
      361 cacttccaga ggatgaacag cagaattgcg ccacgatttc acttcgatgc agtaaatcat
      421 tccctcatag agggtaacta tgtccagctc tcccagtttg tttctataat ttctttctaa
      481 aacctgatgc ccttttccga ctaaaaaatt gagggcttct ttttcgcccc atttcccttt
      541 ttcagtattc aaatgcttca ggccaaaact ttaatttgat tatcggttat ctcatttttt
      601 ttttagaaaa ttatcttagg aaagcaaaaa aagttcaagt taaaaaagaa ggattccgaa
      661 ttatgtctac ttactgatct gccctatcag attcccatcg atagccttgg aaatatagac
      721 agtattgtca ttcagcagat tgacgaatct ctctttcttt gtaatttcac cgcctgcttc
      781 cttgacaata atgagtccag ggcgaagcat tttggaaaaa ttggcctcca gcacctgcgc
      841 aatggaaccg tcagacagtt cgacatatga ccctaccgga tacatcgcaa tcttgttcag
      901 aaacatcctg aggagattca gatcaaatct gttcattaca acactgatca tcgtctttaa
      961 tgcctcatag ggctgaaatt tgcgtctcca tggcctgttg gaaacaagtg cagaaaatgt
     1021 atctgcaatg gaataaatgc aggaggcttc ctcaatttcg tttttcttca tcttctgagg
     1081 atagccggtc ccgtcaaaat tttcgtgatg ctgaagagcc acgagtgcaa tgctgttttt
     1141 cactttatag tttttagtca gaatctgata tccgaaaaga ggatgctttt ttacaacctt
     1201 gtattcttcc tcatttaagg tggctgtctt ttctgaaatg tagccgggaa ttttggacat
     1261 ccctacatcc gaaagaagcc cggccattgc cagatcgacc atctttggtc tggaatagtc
     1321 catagacatt gcaatcagaa gagaataaaa agtagccttc accacctgat tgtaaagata
     1381 atacccatcc aatgaataat tcagaagcat aaaagagaag tttggatttg ctctgacatg
     1441 atcagtaatc tgttcagcaa ttgaacggat ctgattgagc tctattacct tgtcttcggc
     1501 tacctttctg tagagagtct gaacagcatc aaaagaatct ttgtacagcc ttacaaactc
     1561 atccttattc ttttgaagaa tatgaaatga atttctgagt gctgccgttg cagggtct
//
LOCUS       DAOQGJ010001071         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56367_length_1618_cov_2.199616, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001071 DAOQGJ010000000
VERSION     DAOQGJ010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56367_length_1618_cov_2.199616"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(480..1208)
                     /locus_tag="PK683_15150"
     CDS             complement(480..1208)
                     /locus_tag="PK683_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09831.1"
                     /translation="MAKSAKGKKKISPMMIGDAELYQVQYNGGRPYTVLVSRTAKGGR
                     QVKVYRTRQVFNEEWDMDVDAPSDVYEDVPPKPKELLFTWKNPLNLWIAKCPSLYADE
                     AESLGNSILLEIAPEKEKGPRTYIHIGPNVCRFTSKDPIIEYESPIDNNNVPYPAAAS
                     SKEVFFLGEMLRLSREYVKEPVLGRDSGDLKKLPKDVLKWVCIYENLYGTEKERKKWN
                     AQGLYNHPEAKKLQGYKELASSDS"
BASE COUNT          439 a          361 c          331 g          487 t
ORIGIN      
        1 agtggtctgc tttttcagat tcagagacag ttattttagg aaaagccgca gacaaaaaaa
       61 catccggagc tgaattttca cttccattca tcggacaatg aatatctttt ccgtcctgtg
      121 atgtcagtat tgctgattct gcgactgtaa actttttatt ctaatgcgac tttgtcaagt
      181 cgcatcatac agaacaatta ctgttcccct cccctaaagc tcgcctacgg ctgcttcgct
      241 cgcttctagg ggaggggatt ttgtaaaagt cccgcaaacg tggaagctgc tgccggattt
      301 tcctgatgct ttttgaaaat tacagcgtct agacaaacat acctcatttg cataaggtga
      361 gtcatcagta agatcaggtc tgctgcttat cctcagattc agcgcctccg aggatcggca
      421 gtttcaggct gggctcacaa tatattattt cttaatgata atatattcgt ttttcggaat
      481 caggaatcgg aagatgcaag ttccttatat ccttggagtt ttttggcttc aggatgattg
      541 tacaatccct gtgcattcca ttttttcctt tctttctcag ttccgtacag attttcgtaa
      601 atgcagaccc atttcaatac atccttggga agttttttca gatctcctga atcccggcca
      661 agaacgggct ccttcacata ttcacgggaa agtctcagca tctctcctag aaaaaaaacc
      721 tctttgcttg aagcagctgc aggatacgga acattgttgt tgtcaatcgg actctcatat
      781 tctatgattg gatctttgga tgtgaatctg cagacattgg gtccgatatg aatatatgtg
      841 cgaggaccct tttccttttc aggagcgatt tcaaggagaa tggaatttcc gagcgattca
      901 gcctcatctg catagagaga aggacatttg gcaatccaga gattcagagg attcttccag
      961 gtaaacagaa gttctttagg ctttggagga acatcttcat atacatcact gggagcatcc
     1021 acatccatat cccattcttc attgaagacc tgacgggttc tgtaaacttt tacctgcctg
     1081 ccgcccttgg ccgtcctgct gaccagcact gtatatggac gtcccccgtt gtactgaacc
     1141 tggtaaagtt cagcatctcc aatcatcatt ggagatattt tcttttttcc ctttgccgat
     1201 ttagccattt cttattctcc ctttacagaa atatattcag ggcacggaaa tgcaaaagca
     1261 taaaagcggt tcacgcagaa atgtctgaca cgcttcgcta ttcaataaac tcagtgcaga
     1321 ctctagcagt atgcgattat tttctctgtg tgcggcgttt acactgaggt ccaataggct
     1381 agcgaagtca actgcaccaa ccgtcgccta agtttgcaaa aaaacagtgt ctacacaggc
     1441 cctttcctga agaactgaag catcctgaat aatttgattt ttgacagtat ggaaaatgga
     1501 gatttttgca tagcggaaaa aaagagagaa ttaggggaag cgggcagcca tatcattgcc
     1561 tcaaaaagca ctggtgaatt aaaagacaga agcagaaaca tctccatact taatattt
//
LOCUS       DAOQGJ010001072         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56398_length_1617_cov_3.464789, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001072 DAOQGJ010000000
VERSION     DAOQGJ010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56398_length_1617_cov_3.464789"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="PK683_15155"
     CDS             <1..1259
                     /locus_tag="PK683_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNH09832.1"
                     /translation="GIAKSLEEVSTPVSVRWPMFDDSNNPVKALYQMNESNSMNQFIS
                     AGESLKVPGLNFVYADKTGNIAYFACGGLYRKAVRGDRILDGSSGTMEWGEILPYSFQ
                     PKNINPENGLIFTANHIHFKNSQVPIDGYWQADDRSERLTELLYKSTKDKFSIEDMKD
                     YVLDDKFYSAPLYLSYLLPVLEKNEDTLHFIEKQGYEILKKWDGKGNSDEPGAAVFSE
                     FRYQLGRAIFLDEMGEKRFKSVGGTAQIHHFIKAVLKNRNSVFWDDISTPGKKETPEE
                     IVFTAYRRAVAVLMGRMGTDTKLWRWGRLHKLELKHPLGEIPFLRPIFNSGPREISGG
                     TEVVNNLMSKFASENHDVAAGPSVRMLIDFGDIQRIYIINPLGNSGHRLSPDFQNQAD
                     WYKLGKFRVKDLTSMKPEGKPTRLEP"
     gene            1301..>1617
                     /locus_tag="PK683_15160"
     CDS             1301..>1617
                     /locus_tag="PK683_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HNH09833.1"
                     /translation="MKRIESLPFAEAAPGFKEKSEVIRKSYGRYLEEFIEGEIFLHPR
                     AFTIDRSFAQEFSTVFMEANPLFLSAPYAQAHGFRDMLVNPLQVFNVALSLGVQNNSE
                     KAVA"
BASE COUNT          479 a          300 c          383 g          455 t
ORIGIN      
        1 atggaattgc aaaatccctg gaagaggtca gtacgccggt ctcggtccgc tggccgatgt
       61 ttgatgattc caataatcct gtgaaagccc tgtatcagat gaatgaatca aattcaatga
      121 atcagttcat ttctgcaggg gagtcgctga aagttcctgg actgaatttt gtatatgctg
      181 ataagacagg gaacatcgcg tactttgcat gcggaggact gtacaggaaa gcagtgagag
      241 gggacagaat cctggatgga agttcgggaa ctatggaatg gggggagatt cttccttaca
      301 gttttcaacc gaaaaacatc aatcctgaaa acgggttgat ttttactgca aatcatattc
      361 attttaaaaa ttcccaagtg cccatagacg gttactggca ggcggatgac cggtctgaga
      421 gactgacaga acttctgtat aagtctacga aggataaatt ttccattgaa gatatgaaag
      481 actatgttct tgacgacaag ttttactctg caccgctgta tctttcttat ctgctccctg
      541 ttcttgaaaa gaatgaggat actcttcatt ttattgagaa gcagggctat gaaattctga
      601 aaaaatggga tgggaaagga aactcagatg agcccggcgc agctgttttt tctgaatttc
      661 gctatcagct tggcagagcg atttttcttg atgagatggg cgaaaaaaga tttaagtcag
      721 tcggaggaac cgcacagatc catcatttta tcaaggcggt tttaaagaac agaaacagtg
      781 tattttggga tgacattagt actcctggaa aaaaagaaac tcccgaagaa attgtattta
      841 ctgcttatcg aagagcagtg gcagttctta tggggagaat gggaactgat actaaactct
      901 ggagatgggg aagacttcat aaactggaac tgaagcatcc tttgggagaa attccatttt
      961 tgagaccgat attcaattca ggtcctaggg aaatatccgg agggacagaa gttgtcaata
     1021 acctgatgtc caagtttgcg agtgagaatc atgatgtggc ggcaggtcct tctgtaagaa
     1081 tgctcattga tttcggagat attcagagaa tttatattat taatcctctt ggaaattcag
     1141 gacatcgtct gagtcctgat tttcagaatc aggcggactg gtacaaactt ggaaaattca
     1201 gagtaaaaga tctgacttca atgaaaccgg aaggaaagcc gacaagactg gagccttaaa
     1261 aactgcttcc aatcagaggc ggttttttca tttttgtatt atgaaaagaa tagaatcctt
     1321 gccttttgct gaagccgctc caggcttcaa agaaaaatcg gaagtcatcc gcaaatcata
     1381 cggacgttat ctggaagaat tcatcgaggg tgaaattttt ctgcacccta gagcgtttac
     1441 aatagacaga agtttcgctc aggaattttc cactgttttt atggaagcaa atcctctctt
     1501 tctttccgct ccctatgctc aggcacacgg gttcagggat atgcttgtca atcctcttca
     1561 ggtgttcaat gttgcactct ctctcggtgt tcagaacaat tcagagaagg cagttgc
//
LOCUS       DAOQGJ010001073         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56519_length_1615_cov_4.356410, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001073 DAOQGJ010000000
VERSION     DAOQGJ010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56519_length_1615_cov_4.356410"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PK683_15165"
     CDS             <1..849
                     /locus_tag="PK683_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09834.1"
                     /translation="IHQEILSIEVRPQESTVTVDKKTEIGLNGPKGELREHTPFLTVY
                     RLDFLAEIRTGKGTKKVLIELQKAKLSTDIMRFRKYLGEQYSRDSLPIITIYFIGYAF
                     DRTLPKVIEVARVFRDRLTGNDAERQKNEFIDSLTHDSFVIQLFELKTEMRNRLEKAL
                     SVFSQAGKKLKDHIVYYDFPLDDELQQKIVNRLNYAVSDEILKRQMDLEEEILMELQD
                     MERKIENQEKTIEEKDRAIEEKDRTIEEKEKTIEEKEKALEESRKTLDEKDRLILELM
                     KKLENR"
     gene            complement(910..>1615)
                     /locus_tag="PK683_15170"
     CDS             complement(910..>1615)
                     /locus_tag="PK683_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09835.1"
                     /translation="KESSDRLGASVEIFCYAAHYPAVRMQNCSENSHDSGKTAEKALL
                     ISFRNLSESELSDVELVLLLNESLPKADGFKPDHYSIYFKKQAKEGSSFFSLPEGEYF
                     GSLILHRSRTVPFLYSSQSRIIFHFGIQEKYSEKEDKNIIAESGQKKCEILKDSYPVE
                     VLCPKLYVRKKDNPSFELSFRETEYSGSMTGGLWTGSLFMSAVFMNPFGVLIPFAAGP
                     VVLERKVDYNADFKDQ"
BASE COUNT          526 a          308 c          366 g          415 t
ORIGIN      
        1 attcaccagg aaatactttc gattgaagtc aggccccagg aaagtactgt cactgtggac
       61 aaaaaaacag agattggctt gaatgggcct aaaggggaac ttcgtgagca cacacccttt
      121 ctgactgtgt accgtctgga tttccttgca gagatcagga caggcaaagg tacgaagaag
      181 gttctgattg aactgcagaa ggcaaaactg tctacagaca ttatgcgttt tcgcaaatat
      241 ctcggcgagc agtacagcag agactcactg ccgatcatta cgatatattt catcggttat
      301 gcgtttgacc ggactcttcc taaagtgatt gaggtagcga gggtgttccg tgaccgtctg
      361 accggaaacg atgctgaacg tcagaagaat gaatttattg acagtctgac gcatgattcg
      421 tttgtaattc agctgtttga actgaagaca gaaatgcgga accggctgga aaaggcactc
      481 agtgtattca gtcaggccgg gaagaaactt aaggatcata tcgtttatta tgattttcct
      541 cttgacgacg agctgcagca gaagattgtg aaccgtttaa attatgcagt ttcagatgaa
      601 attctgaaac gccaaatgga tctggaagag gaaattctga tggaacttca ggatatggaa
      661 cggaagatcg aaaatcagga gaagacaata gaagaaaagg acagagcaat agaagaaaag
      721 gacagaacaa tagaagaaaa ggaaaaaaca atagaagaaa aagaaaaagc gttggaagag
      781 agtcgaaaaa ccttggatga aaaggacaga ttgattctgg aactgatgaa aaagctggaa
      841 aacagataga tctgttttga cactcgcaag tctgcatttt ttcataacat aaaagctgct
      901 gaagcacctt cactgatcct taaaatcggc gttatagtca acttttctct ccagaacaac
      961 cggtccggcg gcgaatggga taagtacgcc gaaaggattc ataaaaacag ccgacataaa
     1021 aagacttcct gtccagagtc cgcccgtcat tgatccgctg tattcagttt ctctgaacga
     1081 taactcaaaa gagggattgt ccttttttct cacataaagt tttggacaga gaacttctac
     1141 aggataagag tccttcagta tttcacattt cttctgaccg gattcagcaa tgatattctt
     1201 atcttctttt tcagaatact tttcctgaat tccaaaatga aatataatcc ggctctgact
     1261 ggaatataaa aatgggactg ttctggatct gtgcagaatc aatgaaccga agtattctcc
     1321 ttctggaaga gaaaaaaaag aactgccttc ctttgcctgt tttttgaaat atatagaata
     1381 atgatcaggc ttgaatccgt ctgccttcgg gagggattcg ttcagcagca gaaccagttc
     1441 aacatcggaa agctcggatt ctgaaaggtt ccgaaaagag atgagcagtg ccttctctgc
     1501 tgttttgcct gaatcatgag aattctctga gcagttttgc atacggacag caggataatg
     1561 agcagcataa caaaatattt caaccgaagc tcctaatctg tctgaagatt cctta
//
LOCUS       DAOQGJ010001074         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56533_length_1615_cov_3.439103, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001074 DAOQGJ010000000
VERSION     DAOQGJ010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56533_length_1615_cov_3.439103"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK683_15175"
     CDS             <1..202
                     /locus_tag="PK683_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09836.1"
                     /translation="PSFSEKLKGSILIGGAEEEQQILLVRDSEDRWECWHFANWTPGE
                     TVYAGFRFYIESELMRLEEKDI"
     gene            complement(240..>1615)
                     /locus_tag="PK683_15180"
     CDS             complement(240..>1615)
                     /locus_tag="PK683_15180"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNH09837.1"
                     /translation="AIFLVLQFLFPANDQRILRFSSGLLLLISLFYTFDLKKDPGLGI
                     FFSGQVVISDLTIYLNVIYIITALLTVIASPRILNQHGIDFPEFYPLILFAVTGMFFM
                     TTSGDFILTFVGLELLSISLYVLIGMAKNEMSSLEAALKYFLLGAFSSGFMLMGIAFL
                     YGASGSTNIQTAMRFVQGVNFDTDPMIYARIGLGLFLVGVCFKIALAPFHSWTPDVYE
                     GALTTMTGFMASGPKTAAMGLLLVVFGYFTKIDTNSSWTYLLGGIAILSMTLGNVFAL
                     KQENLKRVLAYSSISHAGYVVAGIIVGAKMEVVYYLILYSFMNIAAFSIIAYLENGKQ
                     IVTYDSLRFLVSRKPLSSFGLLVIFFSLGGIPPLGGFWSKLFLFQAIASSDNFMNRLL
                     LIAGVINSAVAIYYYLRLTVSAFMTEDKGDILSENIPISYGLTFASAVSILFVLFSWI
                     VFHPSAL"
BASE COUNT          564 a          328 c          352 g          371 t
ORIGIN      
        1 accaagtttt tcagagaagc ttaaggggag catactgata ggcggtgcag aagaagaaca
       61 gcagatactt ctggtcaggg acagcgaaga cagatgggaa tgctggcact ttgccaattg
      121 gacacctggg gaaactgtgt atgcaggatt cagattttat atagaatcag agctgatgag
      181 actggaagaa aaagatattt agaaactcag gtcggtatgc tgacctgagt tttgtttttt
      241 cagagtgcgg acggatgaaa aacaatccag ctgaacagaa caaacagaat gctcaccgct
      301 gaagcaaatg taagtccata gctgatcggg atgttctctg aaaggatatc tcccttgtct
      361 tcagtcataa atgctgatac agtcagtctg agataataat agatcgcaac tgccgagttg
      421 atcacacctg caatcagaag gagtctgttc ataaaattat ctgaagatgc aatcgcctga
      481 aacagaaaga gcttagacca gaatccgcca agaggcggaa tccctccaag agaaaagaaa
      541 atcacgagga gtccgaagga actgagcggt tttctgctca caaggaaacg aagtgaatca
      601 taggtcacaa tctgttttcc gttctcgaga tatgcgataa tggaaaatgc tgcaatattc
      661 atgaatgaat aaagaatcag ataatagacc acttccatct ttgctcctac aataatccct
      721 gccacaacat atccagcgtg agagatggat gaataggcaa gaactctttt cagattttcc
      781 tgtttcagtg caaatacgtt tccgagagtc atggagagaa tggcaattcc tccaagcagg
      841 taggtccagc tggaatttgt atcgatctta gtaaaatatc cgaacaccac aaggagaagt
      901 cccattgcag cagtcttagg accggatgcc ataaaccctg tcattgtagt caatgctcct
      961 tcatacacat ccggtgtcca ggaatggaat ggagcaagtg caatcttgaa acagactccc
     1021 acaagaaaca gacccagacc gattcttgca tagatcatag gatctgtatc aaaattcaca
     1081 ccctgcacaa aacgcattgc agtctgaata tttgtagatc cgcttgcacc atacagaaat
     1141 gcaataccca tcagcataaa cccggaagag aatgcaccta acaggaaata cttaagcgct
     1201 gcttccagac tggacatctc attttttgcc attccgatca gaacatagag agaaatggaa
     1261 agaagctcaa gaccgacaaa ggtaagaatg aaatctcctg atgtggtcat gaaaaacatt
     1321 ccagtcacgg caaagagaat cagaggataa aattcaggga aatctatccc gtgctgattc
     1381 agtattctcg gcgaagcaat cacagtaaga agagcagtga taatgtaaat tacattaaga
     1441 tatattgtca gatcggaaat cacaacctga ccggaaaaga aaattcccag tccaggatct
     1501 ttcttcagat caaatgtata aaaaagtgaa attaaaagaa gaagcccgga agaaaaacgg
     1561 agaatcctct gatcatttgc gggaaataag aattgaagca ctaaaaatat cgcac
//
LOCUS       DAOQGJ010001075         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56585_length_1614_cov_5.200770, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001075 DAOQGJ010000000
VERSION     DAOQGJ010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56585_length_1614_cov_5.200770"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..718
                     /locus_tag="PK683_15185"
     CDS             299..718
                     /locus_tag="PK683_15185"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNH09838.1"
                     /translation="MNRSRQLGLIAKKIREFYRTLKKEFNQILESEGMTNGEFLMLKS
                     IVQSGNRKVSELAKELGVSMPYITSLSDKMVKKGFIERRQFEEDRRIVVLKLTKEGKK
                     KYSRLDRLILNYMETGFDRLSDQELLTFEKILKKVSD"
     gene            718..>1614
                     /locus_tag="PK683_15190"
     CDS             718..>1614
                     /locus_tag="PK683_15190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH09839.1"
                     /translation="MQHLELIEKKTIMLTVMAVLFFSAVNQTIVGTALPKIVSSLGGM
                     EYFNWVFTIFMLSSSIASILAGRLSDHYGRKPFFIFGILSLILGSLLCGFSESMIQLI
                     IFRGFQGVGAGIIISLSFASVGDLFHQKERGKWQGWLTSVFGLSSLVGPTMGGYISDH
                     FHWNWIFWIFLPLGFIAFFVMIRYYPRQNITPSKLVIDYAGTAVFTLSISSLLYYISI
                     LGIEKASMQREGILLFGIIMLIWFVMIELKAEDPVIPLFLFKKNTFLVSNTVSFFLGM
                     GMFGVIIYIPFFMQGVMQVSATD"
BASE COUNT          463 a          318 c          337 g          496 t
ORIGIN      
        1 attcagctct agtcagtgca cggaaatcat atcaggcggg ccgcagaaat cacataatca
       61 ctgcttttac agaacctctt aaaaaaattc cgcagtcatt ttttctaacc ctgtcactcc
      121 agcggattta tatatacagc atttccccgg tattcccgat tagcataaaa aacagagtca
      181 ctgatatcat atcgtaattt cccctcctgc tcccgcatag atgagcagtc aaagatcgaa
      241 aaatattctc attgacagca ttagttaatt cagctaacag ttaataaggt taagtaagtt
      301 gaacagatca agacagctgg gactgattgc aaagaaaatt cgggaatttt acagaactct
      361 gaaaaaagag ttcaatcaga ttctggaatc ggaagggatg actaacggcg aatttctgat
      421 gctcaaaagt attgttcagt caggaaacag gaaagtctca gaattggcaa aggaattggg
      481 agtttccatg ccttatatca cttcgctttc agataagatg gtaaaaaaag gcttcattga
      541 gcgcagacag ttcgaagagg acagaagaat tgtcgtactt aaactgacaa aggaaggaaa
      601 gaagaaatat tccagactgg acagactgat tttgaattat atggaaacag gatttgacag
      661 actttctgac caggaacttc tgacttttga gaaaatcctt aaaaaggtat ccgactgatg
      721 cagcaccttg aactgattga gaaaaagaca attatgctca ctgtgatggc cgtactgttt
      781 ttttcggctg tcaatcagac cattgttgga acagctcttc ccaaaatcgt ttcctcgctc
      841 ggcggaatgg aatacttcaa ctgggtattt accatattta tgctctcttc cagcatcgca
      901 tccattcttg caggcaggct ttctgatcat tacggcagga agcctttctt cattttcgga
      961 attctgtctc tgatactagg ctcacttctc tgcggttttt ctgaatcaat gatccagctg
     1021 atcattttca gaggttttca aggagtgggt gcaggcatta taatttcatt atctttcgca
     1081 tctgtaggag atctgtttca tcagaaagaa agaggaaaat ggcaggggtg gctgacctcg
     1141 gtatttgggc tgagcagtct ggtcggacct accatgggcg gatacatttc agatcatttt
     1201 cactggaact ggattttctg gattttcctg ccgcttgggt tcattgcttt ttttgtaatg
     1261 atcaggtatt atccaagaca aaacatcacc ccttcaaaac ttgtaatcga ctatgcaggg
     1321 actgcagtct ttactctgag tatttccagc ctgctttatt atatctcaat tcttgggata
     1381 gagaaagctt cgatgcagcg ggaaggcatt cttcttttcg gaatcattat gctcatctgg
     1441 tttgtaatga ttgaactgaa agcggaagat ccggtcattc ctctctttct ctttaagaaa
     1501 aatacttttc tggtatcaaa tacagtcagt tttttccttg gaatgggaat gttcggtgtc
     1561 attatttata ttcctttttt tatgcaggga gttatgcagg tttctgcaac agac
//
LOCUS       DAOQGJ010001076         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56749_length_1612_cov_2.879255, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001076 DAOQGJ010000000
VERSION     DAOQGJ010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56749_length_1612_cov_2.879255"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1612
                     /locus_tag="PK683_15195"
     CDS             <1..>1612
                     /locus_tag="PK683_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09840.1"
                     /translation="DYIKIYRFLSSENYREIRRLETETPDIESEFSHKIELAGLLRKN
                     FLLDEASRLYSELAAAADSPAKKEILQREFNELDVLARLSGVRLQKTSLKSTENSIHS
                     TGIARNWVLFAEKQKEIEEEGHTDSIISFRGRLYDTWKKLETGAEYNFQQLKDDSTAL
                     GESFYSLLDDRDRILIFNIFSRSVPYQRSDEVNELVDSLLKDSSGMKRSLAGYLTLSY
                     AETLFNRGDVDGSLKYLNEYHRKFRSGISDDLEKRGGVLAKKISYLGKKSEFDSEAEN
                     EYFEYYRTGSSEEKAGVYSTVRKMLKDKAEENFSSYHRREIQDLLFFLQGQAIRLNSS
                     DLLFDLAWYRDRLNALSDRFYSRNVKFKDLPEFKPLSKSILSTLPQDQSFVGAVHYGN
                     TTYGVQFENGNFTGKVLFEYDKEDFDSSGTQKEDIYRDNRVLRNDIYDYYRKLNAGES
                     AILKKEFQERRFRKALDKLDKLFKEKYGKPRPEKKRRTYLYLTSFIFKIPFELKDDDD
                     IFLVQSPELLTENRPVSMKKAFQKNSRI"
BASE COUNT          544 a          316 c          361 g          391 t
ORIGIN      
        1 tggattatat aaaaatttac agatttctgt ccagcgaaaa ttacagagaa atccgcagat
       61 tagagacaga aaccccggat attgaatctg aattcagtca taaaatcgag ctggccggtc
      121 tcttaagaaa gaattttctt ctcgatgaag cgtccaggct ttattctgaa cttgccgcag
      181 ctgcagacag ccctgccaaa aaggaaattc ttcagagaga attcaatgag cttgatgtgc
      241 tcgcaagact ttcaggagtc agactgcaga aaacatcatt aaaatctact gaaaacagca
      301 tacacagcac gggcattgca agaaactggg ttcttttcgc agaaaagcag aaagagattg
      361 aggaagaagg tcatacagac agcatcatct ctttccgagg cagactttac gacacatgga
      421 aaaaactgga aacaggtgca gaatacaatt ttcagcagct gaaggatgac agcactgcgc
      481 tcggggaatc attttacagt cttcttgacg acagggacag aatactgata ttcaacatat
      541 tctccagatc tgttccttat cagagaagcg atgaggtcaa tgaacttgtg gacagtcttc
      601 tgaaggactc ttcaggaatg aaaagaagtc ttgccggata tcttacactt tcctatgcag
      661 agactctgtt caacagagga gatgttgacg gctctctgaa atatctgaac gaataccaca
      721 gaaaattcag atcagggatt tcggatgatc tggaaaaaag ggggggggta ctggccaaaa
      781 aaatcagcta cctgggaaag aagtctgaat ttgactcaga agcagaaaat gaatactttg
      841 aatattacag gacaggaagc tctgaagaaa aggcaggcgt ctacagcacg gtaagaaaaa
      901 tgctgaagga caaggctgaa gagaacttca gttcctacca cagaagagaa attcaggatc
      961 tgctgttttt ccttcaggga caggcaatac ggctgaacag ctccgatctt ctttttgacc
     1021 tggcttggta cagggaccga ctgaatgctc tttcagacag attctattcc agaaatgtca
     1081 aattcaagga tcttcctgaa ttcaaacctc tctctaaatc catactttct acccttcccc
     1141 aggatcagtc cttcgtcggt gcagtgcatt acggaaatac cacatacgga gtgcagtttg
     1201 aaaacgggaa ttttacaggc aaggttctgt ttgaatacga caaggaagat tttgacagca
     1261 gcggaactca gaaggaagat atatacagag acaacagggt acttcgtaat gatatttatg
     1321 attattacag aaagctgaat gcaggcgaat cggccatact caaaaaggag tttcaggaaa
     1381 gaaggttcag aaaggccctc gataagctcg ataaactgtt caaggaaaaa tacggaaagc
     1441 cgagacctga aaagaaaaga agaacttatc tgtacctgac aagtttcatt ttcaagattc
     1501 cttttgaact caaggatgat gatgatatct ttcttgttca gagtccggaa ctgctgactg
     1561 aaaacagacc ggtctcaatg aagaaggctt ttcagaaaaa ttccagaatt aa
//
LOCUS       DAOQGJ010001077         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56806_length_1611_cov_3.542416, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001077 DAOQGJ010000000
VERSION     DAOQGJ010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56806_length_1611_cov_3.542416"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1611)
                     /locus_tag="PK683_15200"
     CDS             complement(<1..>1611)
                     /locus_tag="PK683_15200"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNH09841.1"
                     /translation="EIKLPFSLKKGTKISFYKYGDKPKLLNENELKEDTDTVYGRLLV
                     IPEKNDYFFISFPVPSEEFRTENFGEKFYESHGDTFRFSESLISADIHSDTSVKIKET
                     CPALRSDLTFHKDLQMNMPGPDSSDTREVFYQNIQCRTSAECTGYIRKNKYISLTAQF
                     LSSSKEGFHLEFDLYDSGIRKGENMIYVFRDAASGIPGGTFRKTVHRISLPSQIPVQE
                     ITVKSSRRDFPGTSKISYEGSTVIVTDYAVTGHTAQSIEIHIPKKYFSVKDFEKNAVT
                     MNGKELEIRGGGGFSFENLIGSLIILFSLLIGLIPFGIFLGPFIYIFIKMFSSRRSSA
                     GSISSGVSGGVKNSSRSRSNGRDELISMDPDFSEDQFLDRVRDTAHQLTDAWLNENMK
                     PVRSLVSGGVCTRFTVQLHLMKAEGIVNCMQDWQILKVNIRSVDVSTSYSTVHVRLEG
                     KARDLNLSRSLTEEERKLKLKSEPQKLYTEIWSFVRGNSAKTREGFGYFSGNCPNCGA
                     PAKSLTDTNKCSSCDAVFNSGEFDWVLS"
BASE COUNT          426 a          386 c          324 g          475 t
ORIGIN      
        1 cagacagcac ccagtcaaac tctcctgaat tgaaaacagc gtcacagctc gaacatttat
       61 ttgtgtccgt cagagatttc gcaggagctc cgcagttcgg acagttcccg gaaaagtatc
      121 cgaaaccttc tctcgtcttt gcactgttcc ctctgacaaa agaccatatc tcagtgtaca
      181 atttctgcgg ctcagatttc agcttcagtt tcctttcttc ctcagtcaga cttctgctca
      241 gattcagatc ccttgccttc ccttccagac gtacatgtac tgtggaatag gaagtgctca
      301 catccacaga acgtatattc actttgagga tctgccagtc ctgcatacag ttcacaattc
      361 cttcagcttt catcagatga agctgaactg tgaaacgtgt gcagactcct ccggaaacaa
      421 gagaacgtac aggcttcata ttttcattca gccaggcatc tgtcagctga tgagccgtat
      481 ccctcactct gtccagaaac tgatcctcgg agaaatccgg atccatggag attaattcat
      541 cgcgtccatt ggagcgggat ctgcttgaat ttttgacgcc gccgctgacg ccgctgctta
      601 tgctgccggc actgcttctt ctgcttgaaa acatcttaat aaaaatatat atgaaaggac
      661 ccaggaaaat accgaatggg atcaggccga tcagaagcga aaagagtata atgaggcttc
      721 cgatcagatt ctcaaatgag aacccccccc cccctcttat ttccagttcc tttccgttca
      781 tcgtgactgc gtttttctca aagtctttca cagaaaaata ctttttcgga atatgaattt
      841 ctatggactg agcagtatgt ccagtgactg catagtcagt gacaatgaca gtactgccct
      901 cataggaaat tttggaagtt ccaggaaaat cacgtcggga ggactttact gtaatctcct
      961 gaacagggat ttgagaagga agactgattc tgtggactgt ctttctgaat gtcccgcccg
     1021 gaattcccga tgctgcatcc cgaaaaacat aaatcatatt ttctcccttt ctaattcccg
     1081 aatcgtacag gtcgaactcc agatgaaaac cttctttgga ggaagagagg aactgtgctg
     1141 tcagggaaat atatttattc ttcctgatat atcctgtaca ttctgccgaa gttctgcact
     1201 gtatgttctg atagaaaacc tctcttgtat ctgaactgtc cggtccaggc atattcatct
     1261 gcaggtcttt atgaaaagta agatctgacc gtagagcagg gcaggtttcc tttattttta
     1321 cacttgtatc cgaatgaatg tccgcagaaa tgagagactc tgaaaaacgg aatgtatctc
     1381 cgtgactttc ataaaacttt tcgccgaagt tttctgtccg gaattcttct gagggaaccg
     1441 gaaaactgat gaagaaatag tcattcttct cggggatgac aagcagcctt ccatagactg
     1501 tatctgtatc ttctttcagt tcattttcat tcaacagctt cggtttatca ccgtacttgt
     1561 aaaaactgat ttttgttcct tttttcagac tgaacgggag cttgatttca t
//
LOCUS       DAOQGJ010001078         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56818_length_1611_cov_3.138817, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001078 DAOQGJ010000000
VERSION     DAOQGJ010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56818_length_1611_cov_3.138817"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PK683_15205"
     CDS             <1..377
                     /locus_tag="PK683_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09842.1"
                     /translation="NNHSEFEAFADSTYGRRTIGASDSGKNYENFANDARAFTNSRVK
                     RYYDGLDEIYLENAWITYNTRLRSGFDVVVNTDTATRGNMLRHGVNSAIATMALIFEK
                     AAMDLRKCRTACTSSSTYQMKY"
     gene            379..>1611
                     /locus_tag="PK683_15210"
     CDS             379..>1611
                     /locus_tag="PK683_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09843.1"
                     /translation="MKYILFLFILFHCSRNDAVITSKAGSLSYGSYQYLKRLYYSGQT
                     DEDAILSDLKDMYSVSGIATANGLENDRNYEDFLKLEKIGRLTLAGAYRFKELFKEEY
                     GQNSFYRLISYFDISFMEEDALRMIKENTEFSPAVPFLKKKHSDVFIFTLGKKKISLE
                     DLSHFCMKKELVELPALSGQNQKDVLKKIIEKYLFHLLNTDLEKNLKADESELDFIDY
                     ARTARLFTSAKYGFAEKGIYPTEQIRIKLAPKELFEHFHKMMHIFQDIDTLKFSAAVL
                     KNRKDAEDFLNRLKRGEKFEKIAESFSLQGKNREMSTPHTIPGYDRNLPPDRQHQRNY
                     IDLMALDLAKQDLYEYEPVAVKEGYAVIRIYEIKRLDMKLEYKDYRNAVEMDLERRLL
                     NRQFEIDKKDALDRLQFTL"
BASE COUNT          546 a          302 c          349 g          414 t
ORIGIN      
        1 gaaacaatca ctctgaattt gaggcatttg cggattcaac gtatgggaga cgcactatag
       61 gagcatccga ttcaggaaaa aattatgaga attttgcaaa cgatgcaagg gcttttacaa
      121 acagcagagt aaaaagatac tatgacgggc ttgatgaaat ctatctcgaa aatgcctgga
      181 tcacttacaa taccagactc agaagcggat tcgatgtggt tgtaaacact gataccgcca
      241 ccagaggaaa tatgctccgc catggagtaa acagtgcaat tgccacaatg gcactgattt
      301 ttgaaaaggc tgcgatggat ctcagaaaat gcagaactgc ctgcacctct tcaagcactt
      361 atcagatgaa gtactgattt gaagtatatt ctgtttcttt ttattctttt tcactgcagc
      421 agaaatgatg cagtgattac ctccaaagcc ggaagcctct cttacggatc ctatcagtat
      481 ttaaaaagac tgtactattc cgggcagact gatgaggatg caattctttc cgatctgaag
      541 gatatgtatt cagtttccgg cattgcaacg gcaaacggac tcgaaaacga ccgaaactat
      601 gaagattttc tgaaacttga aaagatagga agactcactc ttgccggtgc ttacaggttc
      661 aaagaactct tcaaggaaga atatggacag aactcctttt acaggcttat ctcctacttt
      721 gacatttcct ttatggaaga ggatgcactg agaatgataa aagaaaatac tgaattcagc
      781 ccggcagtgc cgtttctcaa aaagaaacat tccgacgtct tcatatttac tctgggaaaa
      841 aagaaaataa gcctggaaga tctttcccat ttctgcatga aaaaggaact ggtggagctt
      901 cctgcactga gcggacagaa ccagaaggat gttctgaaaa aaattattga aaagtatctg
      961 tttcatcttc tcaacactga tctggaaaaa aatctgaagg cagatgaatc agaactggat
     1021 tttattgact atgcaaggac tgccagactt ttcacaagtg caaaatacgg gtttgctgaa
     1081 aagggaattt atccgactga acagatcaga atcaagctgg ctcccaagga actgttcgaa
     1141 cattttcata agatgatgca tatatttcag gacattgata ctctgaaatt cagtgctgct
     1201 gtactgaaga acagaaaaga tgcagaggat ttcctgaacc gtctgaaaag aggggaaaaa
     1261 tttgaaaaaa ttgctgaaag cttcagtctt cagggaaaga acagggaaat gagcacccct
     1321 cataccatac ctggttacga caggaatctt cctcctgaca gacagcatca gagaaattat
     1381 attgatctga tggctctgga tctggcaaaa caggatcttt atgaatatga accagttgca
     1441 gtgaaagaag ggtatgcagt catcagaatt tatgagatca aaagactgga tatgaagctg
     1501 gaatacaagg attacagaaa cgctgttgaa atggatttgg aaagaaggct tctcaacaga
     1561 caatttgaaa ttgataaaaa agatgccttg gacagactgc agtttacact g
//
LOCUS       DAOQGJ010001079         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56825_length_1611_cov_2.737789, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001079 DAOQGJ010000000
VERSION     DAOQGJ010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56825_length_1611_cov_2.737789"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PK683_15215"
     CDS             <1..597
                     /locus_tag="PK683_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH09844.1"
                     /translation="MALRSISKELPWPGSRCGWIEVFNRKNDPLFDRYIKSLVDAKML
                     EVCSTTLPQMAIPDIYSSPEFIPHLNARNEKYRQRAHQAVEILSQVKGIKVVEPKGGF
                     FLTVLFETGILNDRMKLKISNSEAEKYISGMLANAANDRRFVLNLLASTGICVVPISS
                     FCCRKDGFRVTLLEEDPEAFEKTFRTIADSISDYLKSA"
     gene            594..1376
                     /gene="murI"
                     /locus_tag="PK683_15220"
     CDS             594..1376
                     /gene="murI"
                     /locus_tag="PK683_15220"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNH09845.1"
                     /translation="MNSESDFIIGVMDSGLGGLSVLNELTQEVRYADYIYYGDLANSP
                     YGPKPTKEVERLVRNAVNFLLEKGANAVLLACNSATSAAAEPLRKELSVPVFGMEPAI
                     KPALSENPGEKVGVLATSLTLREDKFQTLGESLSAAERIHPVNCDGLASLIDLGDFPE
                     IMNFLNRIFSDLNENKIQNIVLGCTHYVLMESVFRKEKPELRIYHGNFGTVRHIKNTL
                     NLPDADREPKLELYLNGGSEKDFELAFNYLNSSAEKETRYVK"
     gene            1366..>1611
                     /locus_tag="PK683_15225"
     CDS             1366..>1611
                     /locus_tag="PK683_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNH09846.1"
                     /translation="MSNELTYKAAGVDTHAGQEFVSRIKANVHSTHNPRVLGGLGGFS
                     AAYDVSFLKGYKEPVLLSGTDGVGTKLEIARLLNKHDT"
BASE COUNT          501 a          360 c          352 g          398 t
ORIGIN      
        1 atggcactgc gttcgatctc aaaggaactt ccatggcctg gctccagatg cggctggata
       61 gaagtgttca acagaaaaaa tgatcccctt ttcgacaggt acatcaagtc tcttgtagat
      121 gcaaaaatgc tggaagtctg ctctactacc ctcccccaga tggcaatacc ggatatttat
      181 tcttcacctg aatttatccc gcatctgaat gcaagaaacg aaaaatacag acagagagct
      241 catcaggctg tggaaattct gagtcaagtc aaaggaatca aggtagtgga accgaaaggc
      301 ggattctttc tgactgtact ttttgaaaca ggaatactga acgacagaat gaagctgaaa
      361 atcagcaatt cagaagcaga gaaatacatc tcaggaatgc ttgcaaatgc cgcaaatgac
      421 aggaggtttg ttctgaacct tctggcttcc actggaatct gtgttgttcc aatttcctcc
      481 ttctgctgca gaaaggacgg attcagagtc actcttctcg aagaagatcc tgaagccttt
      541 gaaaaaacgt tcagaacaat agctgattcc atttccgatt atctgaaatc tgcatgaatt
      601 ccgagtctga tttcattata ggcgtcatgg actccggact gggaggactc tcagttctga
      661 atgaactcac gcaggaagtc agatatgcag attatatata ttacggagat ctggcgaaca
      721 gtccttacgg accgaagccc accaaggaag tcgaaagact tgtcagaaat gcagtgaact
      781 tccttctgga aaaaggagcc aatgcagttc tgctcgcctg caattctgcc acatccgcag
      841 cagcagaacc tctcagaaaa gaactttctg ttcctgtttt cggaatggaa cctgcaatca
      901 aaccggctct ctcagaaaat cccggcgaaa aggtaggagt gcttgcaacc tctctcacac
      961 tgagagaaga caagtttcag actctgggag aatcactttc tgccgcagaa aggattcatc
     1021 cggtcaactg tgacggactt gcctcactca tagatctcgg ggactttccg gagattatga
     1081 actttctgaa cagaatattc tcagatctta atgaaaataa aatacaaaat attgttttgg
     1141 gatgcacaca ttatgtgctg atggaatccg tcttcagaaa ggaaaaacct gaactcagga
     1201 tttaccatgg aaatttcgga acagtgcgcc atataaaaaa tacactcaat cttccggatg
     1261 cagacagaga accgaaactc gaactgtatc tgaacggagg ttctgagaaa gattttgaac
     1321 tcgcattcaa ttatctcaac tcttcagcag aaaaggaaac cagatatgtc aaatgaactt
     1381 acatacaagg cagcaggagt agatactcac gcaggacagg aatttgtcag cagaatcaag
     1441 gcaaatgttc attccaccca caatcccagg gttctaggag gactcggcgg attttcagct
     1501 gcttatgatg tgagctttct caaaggatac aaggagcctg ttcttctttc aggcactgac
     1561 ggagtgggca caaaactcga aatcgcacgt cttctgaaca agcatgatac t
//
LOCUS       DAOQGJ010001080         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56833_length_1611_cov_2.544987, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001080 DAOQGJ010000000
VERSION     DAOQGJ010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56833_length_1611_cov_2.544987"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK683_15230"
     CDS             <1..209
                     /locus_tag="PK683_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09847.1"
                     /translation="LQLSALFLKDGYFFPYILISSDRPYSQGTIDRMSLRKDLSGSLM
                     NNPTDSGFMTRLFGQACFAVLPLN"
     gene            511..1593
                     /locus_tag="PK683_15235"
     CDS             511..1593
                     /locus_tag="PK683_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09848.1"
                     /translation="MEEYRLTNIKIKGLFEKKNYNIEINENKLVFFDENGAGKTTILN
                     IISAFLSKEWYRLYRYPFEIIECSINERIYSFENKKDLLYDKEKFRNFVRDKGNASTE
                     KSDAIKILEAKSLKFLVENEFNEKFLFVPKNSDLKDEEMDLEEYDAKSLSEYLNGQYE
                     EYTKSIYSDLESFKKMRFYPILFLPTYRVIERDAAFLLDTDIEGRSKSLDKEMITEIF
                     EFGMGKVSGIIDDFSKKEKSKLIDFIELCNGYFSEAGNKSFFWDEAENEVILQQKDSK
                     IFFDDLSSGEKQIVSIFSYLLLLEKDFFVIIDEPEISLSLVWQKKILSDIMNYSKGFL
                     CATHSPFVSKDEKINEYQYMLEYFKE"
BASE COUNT          553 a          236 c          301 g          521 t
ORIGIN      
        1 ctctgcagct gtcggcttta tttttgaagg acggatattt cttcccgtat atccttattt
       61 catcagatcg tccatacagc caggggacaa ttgaccggat gtctttgcgc aaagacctgt
      121 ctggctctct gatgaataat cccacagatt caggctttat gactcgtctt tttggtcaag
      181 cctgttttgc tgttttaccc ttgaattgag gacagttttc agaaagactg gagatcaaag
      241 cggttagagc gtattgagca aaaatggtca ctaaaattag tattgacaag gttttcagga
      301 ttctgtcttt agtgcagtat tatttagctg tttcttgcga gcggaataaa gttgacctat
      361 caaattctat agtaaaaaga gtttactctt ttcaccaaaa gtgaaaactt ggagtgggca
      421 ttcacttcaa taaaaatcga gtggtgtcga ttgtggactg gtctaaatag ggcagaaaat
      481 atcagtacct ttaattgtag gtaaaaaaat atggaagaat accgactgac taacataaaa
      541 atcaaaggtc tatttgaaaa gaaaaattat aatattgaaa tcaatgaaaa taaactggtt
      601 ttcttcgatg aaaacggagc aggaaaaaca actatcctga atatcatttc tgcattttta
      661 tctaaagaat ggtacagact ttatcgttat ccttttgaaa ttattgagtg ttcaatcaat
      721 gaaagaatct actcctttga aaataaaaaa gatcttcttt atgataaaga aaaatttaga
      781 aactttgtcc gtgataaagg aaatgcttca acggaaaaat cagatgccat caaaatattg
      841 gaagccaaat ctcttaaatt tttagtagaa aatgagttta atgaaaaatt cttgtttgtt
      901 cctaaaaatt cagatttgaa agatgaagaa atggatcttg aggaatacga tgcaaagtct
      961 ttgtcagagt atttgaacgg gcaatatgag gaatatacaa aatcaatcta ttccgattta
     1021 gaaagtttta aaaaaatgag attttatccg atattgtttc tgcctacata tagggttatt
     1081 gaaagggatg ctgcatttct tttagataca gatattgaag gcagatccaa gtcattggat
     1141 aaggagatga ttactgaaat ctttgaattt ggaatgggca aagtcagtgg gatcatcgac
     1201 gatttctcga aaaaagaaaa aagcaaactg attgacttta tcgaactttg caacggatat
     1261 ttttcagaag caggaaacaa atcttttttc tgggatgaag cagaaaatga agtcattctt
     1321 cagcaaaagg attcaaaaat tttctttgat gatctttcat ccggagaaaa acaaattgta
     1381 tctattttct cttatctcct tctgcttgaa aaagactttt ttgtaattat tgatgaaccg
     1441 gagatttctc tttctttagt atggcaaaag aaaattcttt ctgatattat gaattactca
     1501 aaaggctttc tttgtgctac gcattctcct tttgtttcaa aagatgaaaa aatcaatgaa
     1561 taccaatata tgctggaata cttcaaggaa taaaagagat ggcgttatcg g
//
LOCUS       DAOQGJ010001081         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56838_length_1611_cov_2.424807, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001081 DAOQGJ010000000
VERSION     DAOQGJ010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56838_length_1611_cov_2.424807"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK683_15240"
     CDS             <1..268
                     /locus_tag="PK683_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09849.1"
                     /translation="ERYRKLGCRFNGSLKGQDIDKYIIMDNSAEKIFQSLFQKRNLSI
                     RRLQKIKKISRTNADLDGSENVREEHVFKALSLTKPFQFPMLAA"
     gene            complement(277..1248)
                     /locus_tag="PK683_15245"
     CDS             complement(277..1248)
                     /locus_tag="PK683_15245"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH09850.1"
                     /translation="MSFFNFLKSREVSFFRKTAFYLFLFSLFSGFLFFAGIVSQVFFF
                     GLLAGSFSFAFIFLGINFRQISLIKKYLGIVQYLDLDPLDNFDAKNKYAQDLLFSGDI
                     NDDAYEVISYYRPLGLLGGDLYYQKRDNKGYYWFAIGDSSGHNIYSHLFSSMILMELN
                     YLIANEDSPKGVNQKINSNLRQKKQFESLPLYASMSLLRADSRGNFTHYGQHPNMVVF
                     RAEQNKTEIVETGGSFIGLESMIPEPDHPLEFHLNKGDILFTFTDGLYEQKNSENRYF
                     GTKLFQFLSEEPKEDLGILVSRLFCEVNLFTEGKISDDISLLIIRKK"
     gene            complement(1248..>1611)
                     /locus_tag="PK683_15250"
     CDS             complement(1248..>1611)
                     /locus_tag="PK683_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09851.1"
                     /translation="KERKRKGEETVEELIIGRGTWTAEGNWVLLSTSNIEKKMMKNNE
                     NAEVKSFDTAHQLLYYSVIDRNMIVPMVNDRAYTEREFGVKDGAERPYDENGRGFRTF
                     LKNYSEKEYHSHSYYRAD"
BASE COUNT          495 a          333 c          322 g          461 t
ORIGIN      
        1 agaaaggtac agaaaacttg ggtgcagatt caacgggtca ctgaaaggac aggacataga
       61 taaatatatt ataatggaca acagtgcaga aaaaatattt cagtcccttt tccagaagag
      121 aaatctgagc ataaggagac ttcagaaaat caagaaaata tcgagaacaa atgcagattt
      181 ggacggctca gagaatgtca gggaagaaca tgttttcaag gcactgagcc tgacaaaacc
      241 ttttcagttt ccgatgcttg cagcctgatc ggcagtctat tttttacgaa taattagaag
      301 agaaatgtcg tcggatattt ttccttcagt aaacagattc acctcgcaga aaagacggga
      361 aacaagtatt cccagatcct ctttaggttc ctctgacagg aattgaaaca gtttcgttcc
      421 gaaatatctg ttttcgctgt ttttctgctc atacaggccg tcagtgaatg taaaaaggat
      481 gtctcccttg ttcagatgaa attccagagg atgatcaggt tcaggaatca tagactcaag
      541 tccgatgaaa ctccctccag tctctacgat ttcagtcttg ttctgttccg ccctgaatac
      601 gaccatattg ggatgctgcc cgtaatgcgt aaagtttccc ctgctgtccg cacggagaag
      661 cgacatggat gcatacagag gaagactttc aaactgtttc ttctgacgca ggttgctgtt
      721 gattttctga ttcactccct tgggagaatc ctcatttgca atgagatagt tcagctccat
      781 caggatcatg gaactgaaaa gatgggaata gatattgtgc ccggaactgt cgcctatggc
      841 aaaccagtaa tagcctttat tatctctttt ctgataatat aaatcgccac caagaagtcc
      901 gagaggtctg taatagctga tcacttcata tgcatcatca ttgatgtccc cgctgaacag
      961 aagatcctgt gcatatttgt tctttgcatc aaaattatcg agaggatcga gatcgaggta
     1021 ctgaacgatt ccgaggtatt ttttgatcag actgatctgt ctgaaattga tcccgagaaa
     1081 aatgaacgca aacgaaaaac ttccggcaag caggccgaaa aagaagacct gagagacaat
     1141 tcctgcaaag aacagaaaac cggaaaataa cgaaaaaaga aaaagataaa aggcagtttt
     1201 tctgaaaaag gaaacttctc tgctttttaa aaaattaaaa aatgacatca gtctgcccgg
     1261 taataggaat gggaatgata ttctttttca gagtaatttt tgagaaatgt acgaaagcct
     1321 ctgccgtttt catcatatgg tctttccgct ccgtctttga ctccgaattc cctttcagta
     1381 tatgccctgt cattcaccat aggcacaatc atattcctgt caatcacact atagtacaga
     1441 agctgatgag cagtgtcaaa tgatttaact tcagcatttt cattattttt catcattttt
     1501 ttctcaatat tggatgtgga aagaagaacc cagttccctt ctgcagtcca ggtcccccga
     1561 ccgataatta actcttcgac agtttcctcg ccttttcttt ttctttcttt g
//
LOCUS       DAOQGJ010001082         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56869_length_1610_cov_3.823151, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001082 DAOQGJ010000000
VERSION     DAOQGJ010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56869_length_1610_cov_3.823151"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PK683_15255"
     CDS             <1..1396
                     /locus_tag="PK683_15255"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNH09852.1"
                     /translation="ISIMAIYHFAVYFFRRKDRSALAFSLFCTAMDFRLLGFNEKILN
                     NFFTGNSFLILNRIEYLAYYLAVPFFAGFQFSIFPNEFSRTALKFFWMVSSVFCGIVL
                     LTDSSFYTQTAFYYHLYVFIFMIYTSFVMVQALRKKREGAKIFLFGLFILFAGTVNDI
                     LHSMTLIRSEYLAPQSLFAFIFIQSVMLSRRFSKGFNLAENLSLELTELNAGLERKVS
                     DRTKELSLQKEVAEQANLIKDKFVSIVSHDLRSPLIGVSNVLDILDRKDILDSEEERS
                     HFLKMGRESVQYSLKMIRELLSLSRIETGSVKVSRQKIRFQDLADSLKNEAVIQASLK
                     NITIRQEILNDTPVFADLDLLTQVLRNLIGNAVKFTREGGQIILRFSTVQNWDLIEIE
                     DNGVGMSSEQLNMIFEPGTRKVTSGTSGEAGSGMGLFICRYIMEAHKGRIEFRSAEGK
                     GTCCSLYLPAAQSA"
     gene            complement(1416..>1610)
                     /locus_tag="PK683_15260"
     CDS             complement(1416..>1610)
                     /locus_tag="PK683_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH09853.1"
                     /translation="VLKPLELEIVHYISSGHTYRQIGEKIGRTEKTVEYYTGRIRKKI
                     GVQNNSELLTFISRNYFKYN"
BASE COUNT          442 a          306 c          374 g          488 t
ORIGIN      
        1 catcagcatt atggcaatct accatttcgc tgtgtatttt ttcaggagaa aagacaggtc
       61 tgctcttgca ttcagtcttt tctgcactgc aatggatttc cgtcttctgg ggttcaatga
      121 aaaaatattg aataatttct ttacagggaa ttctttcctg attctcaata gaatcgagta
      181 tctggcatat tatcttgcag ttcctttttt tgccggtttt cagttcagta tttttccgaa
      241 cgaattcagc agaactgcac tgaaattctt ctggatggtt tcttcagtgt tctgcggtat
      301 tgttctcctc acggacagtt ctttttacac acagaccgct ttctattatc atttatatgt
      361 atttatattt atgatatata cttcatttgt gatggtacag gccctgagga aaaaaagaga
      421 gggtgcaaag atcttcctat tcggactctt tattcttttt gcgggaacag ttaatgacat
      481 tctgcattct atgaccttaa tccgttcgga ataccttgcc cctcagagtc tttttgcatt
      541 catattcatt cagtctgtga tgctttccag aaggttttcc aaaggattta atctggcgga
      601 gaatctgagt ctggaactga ctgaactgaa tgcaggtctg gaaaggaagg tgagcgacag
      661 gacaaaagaa ctcagcctgc agaaagaagt tgcagaacag gcaaatctga tcaaggacaa
      721 atttgtttct atcgtatctc atgatctcag gtccccactg atcggtgtat ccaacgtact
      781 ggatatcctg gacaggaagg atatacttga ttctgaagaa gaaagaagcc atttcctgaa
      841 aatgggcaga gagagcgttc agtattcatt gaagatgatc agggaactcc ttagtctcag
      901 caggattgaa accggttcgg tcaaagtcag caggcagaaa atccgttttc aggatctggc
      961 agacagtctg aagaatgagg cagtgattca ggcatcatta aaaaatatta caattagaca
     1021 ggagatactg aatgatactc ctgtttttgc agatctggat cttctgactc aggtgctccg
     1081 aaatctcatc ggaaatgcag tcaagtttac tagggaaggc ggtcagatca ttctcagatt
     1141 ttcaacagta cagaactggg atctgattga gattgaagac aatggagtag ggatgagttc
     1201 agaacagctg aatatgattt ttgaacctgg gacaaggaaa gtgacttccg gaacttcggg
     1261 agaggctggg agcgggatgg gacttttcat ctgcaggtac attatggaag cgcataaggg
     1321 cagaatcgaa ttcaggagtg cagaaggaaa agggacctgc tgttcattgt atcttccagc
     1381 agcccagagt gcgtgaccgc actctgggct gctggtcagt tgtacttaaa atagtttctg
     1441 ctgatgaaag tcagaagttc tgaattgttc tgcacgccga tttttttcct gattctgccg
     1501 gtatagtatt ccacagtttt ttcggtgcgg ccaatttttt caccgatctg tcggtaggta
     1561 tggcctgaag aaatataatg cacaatctca agttcgagag gcttcaatac
//
LOCUS       DAOQGJ010001083         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_56870_length_1610_cov_3.801286, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001083 DAOQGJ010000000
VERSION     DAOQGJ010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56870_length_1610_cov_3.801286"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PK683_15265"
     CDS             <1..829
                     /locus_tag="PK683_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNH09854.1"
                     /translation="IVQSEAFSAIFITLATVTLILLLFYRNILAALISLAPLLLGIVT
                     MLGIMSLLNLKVNFMNILVFPIIIGYAIQNGIYIYFRYMEEKKIITALAKVGPAVIAS
                     TLTTLVGWSVLLIAEHRGLHSIGVAASIGIGVTLIVALTLLPALLEKFFAEKEDSSLR
                     GVGINFDTSLPKIDTVPDAVYTEKLVEFQEKAEQFETPKAEEKAEIAENKEETAETKD
                     IEENSDDDKIVEKTETDRKAETKKKAVTKKKSVSRKKTETAAKKKGGKGEKKSSQNK"
     gene            801..>1610
                     /locus_tag="PK683_15270"
     CDS             801..>1610
                     /locus_tag="PK683_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNH09855.1"
                     /translation="MRRRVHRINKAGDLNDLKLIEEDLPDPSETEITVKTEAVGLNFA
                     DIFALTGLYSATPKGSFIPGLEYSGTVIKTGRDVSRFKAGDKVMGVTRFGAYADHLNI
                     DEKYAYPIPAGWTMEEGAAFIAQGLTAYYALIELGGLKKGQTVLIHSAAGGVGILANR
                     IARKFEAYTIGCVGNESKLEVLKQEGFMDGFVRGEDFRYRLEKTLNNRDLHLVLECNG
                     GSIFQDSYTALSPGGRIVTYGSANFTPSGSSPNWFVTIFNYLTRPMIDPLHM"
BASE COUNT          496 a          304 c          391 g          419 t
ORIGIN      
        1 gattgttcag tcagaggcat tttctgcgat ttttatcact cttgctacag taacgctgat
       61 tcttcttctg ttttacagaa acattcttgc agcacttatt tcacttgctc cgcttctgct
      121 cggcatcgtg acaatgcttg gaataatgag ccttctgaat ctgaaggtaa attttatgaa
      181 tatattggta tttccaatta ttatcgggta cgcgatacag aatggaatat atatatattt
      241 cagatatatg gaagagaaaa aaatcattac agcactggct aaggtgggac cggctgtcat
      301 tgcctccact ctcactactc ttgtgggatg gtcagtactt ctgattgcag aacacagagg
      361 acttcattcc atcggagttg ctgcaagcat cggaatcggt gtaacgctga ttgttgcgct
      421 cactcttctt cctgctcttc tggaaaagtt ttttgcagaa aaggaagatt cttcccttag
      481 aggtgtcgga attaattttg ataccagcct tccgaaaata gacactgtac cggatgcagt
      541 gtatacagag aaacttgtgg aatttcagga aaaggcagaa cagtttgaaa cacctaaagc
      601 cgaggaaaaa gctgagattg cagaaaacaa agaagaaact gctgagacca aagacattga
      661 agaaaactct gatgatgaca agattgttga aaagactgaa acagacagga aggctgaaac
      721 aaagaaaaaa gccgtaacga aaaagaaatc agtttcaagg aaaaagactg aaacagcagc
      781 aaaaaagaaa ggcggaaaag gtgagaagaa gagttcacag aataaataag gcaggcgatc
      841 tgaatgatct taaactgatt gaagaggatc ttcctgatcc gtctgaaaca gaaattacag
      901 ttaagacaga agctgtcggt ctgaattttg cagatatatt tgcactgact ggactttaca
      961 gtgcgactcc gaagggaagt ttcattcccg gcttggaata ttccggcact gttattaaga
     1021 cagggcggga tgtcagtcgc ttcaaggctg gggacaaggt gatgggagtg accagattcg
     1081 gcgcgtatgc agatcatctg aatatcgatg aaaagtatgc ttatcccatt cctgccggct
     1141 ggacgatgga ggaaggagcg gcttttattg cacagggact cactgcctac tatgccctga
     1201 tagaacttgg cggattgaag aaaggtcaga ctgtactgat tcacagtgca gccggaggtg
     1261 tggggatact tgccaacagg attgccagaa aatttgaagc ttatacaatc ggctgtgtcg
     1321 gaaatgaatc caagctggaa gttctgaagc aggaaggatt tatggatggt tttgtgaggg
     1381 gagaagattt cagatacaga cttgaaaaaa ccctgaacaa tcgggatctt catcttgtgc
     1441 ttgagtgcaa cggaggaagc atatttcagg acagttatac ggccttgtct ccaggaggac
     1501 ggatagtcac ttacggctcc gcaaatttta ctccgtcagg aagttctccc aactggtttg
     1561 ttacaatatt caactacctc acaaggccga tgattgatcc ccttcatatg
//
LOCUS       DAOQGJ010001084         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57079_length_1607_cov_3.350515, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001084 DAOQGJ010000000
VERSION     DAOQGJ010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57079_length_1607_cov_3.350515"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..327)
                     /locus_tag="PK683_15275"
     tRNA            complement(254..327)
                     /locus_tag="PK683_15275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Arg,seq:cct)
     gene            complement(337..972)
                     /locus_tag="PK683_15280"
     CDS             complement(337..972)
                     /locus_tag="PK683_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH09856.1"
                     /translation="MMLEAMFGKFGKVFQPNQIIFCEYEPGNDFYLIQEGQVKITKTV
                     GNSIKTLDILEAGDIFGEMAILEEQPRSATAVAIDEVKALNFNRANFELLMTKNPQLA
                     LRVLTIFSTRIYDQKRRLQILLLDDMNGKVADVFVMLAEKQNPHEMTKEITLKITVDD
                     VAHWCGQPVIEVQKVVAQYKKSGKLDVFADRIVVRNIQEFQRTVTQKRKEK"
     gene            complement(977..>1607)
                     /locus_tag="PK683_15285"
     CDS             complement(977..>1607)
                     /locus_tag="PK683_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cAMP-binding protein"
                     /protein_id="HNH09857.1"
                     /translation="LMNVAEVFHRNGNYDHAIYAYERYLAHYQTGAYVGRAKELLALA
                     KRSSMFPVNMPELSYEMEKKHAAGGYAPVPESGPTKALKELQTKADEHFKSGKYAEAA
                     AIYKNLSDTKDSKGPDDDKIIEQALHSLGLSQEWLGKLDEAYATLSGYVKKYGKGSKV
                     KESILHLGMISEKKGEKEKAVMLYNKILGLPPEDSVTADARNKIAALKG"
BASE COUNT          415 a          364 c          304 g          524 t
ORIGIN      
        1 tgacttctgc gagagtcaag cagagaacag aataagaatg gatataatgt attttttcat
       61 attgtcagta atgatttcca gtgccttcaa tcctcagaac agtcctccac tgatgcagtg
      121 cttatgatat gttttttctg aagaaacgca gtttgcatag gcattgctct gttactgtca
      181 ctccgacagc gttcgaaacg ccgtgattaa tacacttctg cagtaagacc aatcggtata
      241 agaggatcat ttatgccccc gactggattc gaaccagtga ccctttgttt aggaaacaaa
      301 tgctctatcc acctgagcta cgggagcggt ctaaaattac ttttcttttc ttttctgtgt
      361 aactgttctt tggaattcct gaatgtttct gactacaatt ctgtctgcaa acacatccag
      421 ttttcctgac tttttatact gagcaaccac tttctgaacc tctatgacag gctgtccgca
      481 ccaatgtgcg acatcgtcca cagtgatttt cagagtgatt tcttttgtca tttcatgagg
      541 gttctgtttt tctgcaagca tcacaaaaac atcggcgact tttccgttca tatcatccag
      601 caggagaatc tgcagacgtc ttttctggtc ataaattcta gtggaaaaaa tggtaagtac
      661 tcgaagggcc agctgaggat ttttggtcat cagaagttca aaattggccc tgttaaagtt
      721 caatgccttt acttcatcaa tggcaactgc tgttgcggaa cgaggctgtt cttcaagtat
      781 tgccatttct ccgaagatat cacctgcttc aagaatatct aaagttttga tggaatttcc
      841 gactgtctta gtaattttta cctgtccttc ctgaatcaga taaaaatcgt ttcccggctc
      901 atattcacag aaaatgatct gattgggctg aaaaaccttt ccgaactttc caaacattgc
      961 ttcaagcatc atatctttaa ccctttaaag ctgcgatttt gtttctcgca tcagcagtga
     1021 cagaatcttc aggtggaagt cccaaaatct tattatagag cataacagct ttctctttct
     1081 cgcctttttt ctcagaaatc attccgagat ggagaattga ctcttttacc ttgctgccct
     1141 tcccatattt tttaacatat ccggacaagg ttgcatacgc ctcatcgagt ttcccaagcc
     1201 attcctgaga aagtcccagt gaatggaggg cctgttctat gattttatcg tcatcgggtc
     1261 ccttggaatc ttttgtatct gaaagatttt tatagatggc tgccgcttca gcgtatttac
     1321 cgcttttaaa atgctcgtca gccttggtct gcagttcttt aagagccttg gtcggtccgg
     1381 attcaggcac aggagcataa ccaccggcag catgtttctt ttccatctca taggaaagtt
     1441 caggcatatt tacagggaac atagaacttc ttttggctaa agccagcagt tcttttgccc
     1501 tgcccacata agccccggtc tgataatgtg ctaaatatct ttcgtatgca taaattgcat
     1561 gatcgtaatt tccgtttcga tgaaacacct cagcaacatt catcagt
//
LOCUS       DAOQGJ010001085         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57106_length_1607_cov_2.207474, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001085 DAOQGJ010000000
VERSION     DAOQGJ010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57106_length_1607_cov_2.207474"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PK683_15290"
     CDS             <1..601
                     /locus_tag="PK683_15290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNH09858.1"
                     /translation="GGVVAYLSVNGDYKGYFLFKEKIRPEVPGLIAKLKKQGKEIAIL
                     SGDKEVNVRKVADELGITRFHYEMKPEDKAAILDDDRLHGVTTIMTGDGINDSLSISK
                     AAVGISHDHGEDISIDNSEIVLLNGRLDGISTVIELAEITDRVVKENIIISLGYNLIM
                     IPLAAAGFMLPVICALFMTMSSLTVILNSSSIFLRGRNL"
     gene            598..741
                     /gene="ccoS"
                     /locus_tag="PK683_15295"
     CDS             598..741
                     /gene="ccoS"
                     /locus_tag="PK683_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNH09859.1"
                     /translation="MNAFYITIPIALAISAAAFAVLLMSLRKGQYKDIEAAKYRIFFD
                     EEK"
     gene            744..1460
                     /locus_tag="PK683_15300"
     CDS             744..1460
                     /locus_tag="PK683_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNH09860.1"
                     /translation="MESFSLAAVLMMGLASSFHCVGMCAPLAGCATSVCPSRNKGFFV
                     LQAGRLISYTVIGGLIGLGGSAVNGIVESGTVIRISAVISIALVLLSVIVNIKLGFAE
                     KFAAPLMDMIGRGKNAYLGNFLLGLVTGFLPCGALYPAYAVSLAAGNAYLGAAGMSLF
                     FLGTLPGNSVIFLSYNILKRKLSTGQLKMIYLVLGVVGISSLYVKIGDPEKAKTCHTP
                     GIEHHEEHSESKRQIQQSPR"
BASE COUNT          423 a          320 c          393 g          471 t
ORIGIN      
        1 aggaggagtc gttgcctatc tttctgtaaa cggagactat aagggttatt ttcttttcaa
       61 ggaaaaaatc cggccggaag ttcccgggct gattgcaaaa ctgaagaaac agggaaagga
      121 gattgccatt ctgtcgggag acaaggaggt caatgtcagg aaggttgccg atgaactcgg
      181 cattacgcgt tttcattatg aaatgaagcc tgaagacaag gcagcaattc ttgatgatga
      241 cagacttcat ggagtgacta cgattatgac aggagacggg atcaatgaca gtctttcgat
      301 ttccaaggcg gcagtcggca tttcccatga tcacggagag gacatttcaa tagacaattc
      361 ggaaatagtt cttctgaacg gccgtctgga cggaatttcc actgtgattg aacttgccga
      421 gatcacggac agggtggtca aggaaaacat tataatctca ttgggttata atttaataat
      481 gataccgctt gcagctgcag gcttcatgct gccggtgatc tgtgctttgt ttatgacgat
      541 gagttcgctg actgtcatac tgaattcatc atcaattttt ctgaggggaa gaaacttatg
      601 aacgcttttt acattacaat tccgatcgcg ctggctatat ctgcagcagc atttgctgta
      661 ctgctcatgt ctctgcgcaa aggtcagtac aaggatatcg aggcggccaa gtacagaatt
      721 ttttttgacg aggagaagtg aatatggaat ccttcagcct ggccgcagta cttatgatgg
      781 gactggcatc ctcatttcac tgtgtaggaa tgtgtgctcc gctcgcaggc tgtgcaactt
      841 ctgtatgtcc gagcaggaat aagggatttt ttgtacttca ggcaggcagg ctaatttcct
      901 atactgtgat cgggggactg atcggtctag gcgggagtgc tgtaaacggg atcgtggagt
      961 ctggaactgt catcagaatt tctgcggtaa tttccattgc gcttgtgctt ctttctgtga
     1021 tagtgaatat caagcttgga tttgcggaaa aatttgcagc tcctctgatg gatatgatcg
     1081 gtcggggaaa aaatgcatat cttggaaatt ttcttctggg acttgttaca gggtttcttc
     1141 cctgcggggc tctttatcct gcatatgccg tgagtcttgc tgcaggcaat gcatatcttg
     1201 gagcagcagg aatgtcgctt ttctttctcg gcactcttcc gggaaattcc gtcatattcc
     1261 tttcatacaa tatactgaaa agaaaactca gtactgggca gcttaagatg atttatcttg
     1321 ttctgggtgt tgtcggaatt tcatctctgt atgtcaagat cggtgatccc gaaaaagcaa
     1381 aaacctgcca tactcctgga attgaacatc atgaagaaca ttcagaatcg aagaggcaga
     1441 ttcagcagag tccccgctga ttcactgttt ctgctgcata attataaata actgattata
     1501 tactttcttt ctatgctgct ttcatgaaat atatgcgtaa aagaagagcg gaaatccctg
     1561 aaatataaaa aacgtattcc ggtccgaatt cctttcccta gccggtc
//
LOCUS       DAOQGJ010001086         1606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57146_length_1606_cov_2.714378, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001086 DAOQGJ010000000
VERSION     DAOQGJ010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57146_length_1606_cov_2.714378"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PK683_15305"
     CDS             <1..945
                     /locus_tag="PK683_15305"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09861.1"
                     /translation="PEEAPNTVLGTKIEEAYEFYRKGMEKFLISDETSLLEAAAFFRE
                     ALRIDPGFARARARLSSAYQILVSSDFNDGKEEEYLTLGYNEALKAYQSAPNSSETNI
                     AVGQFNLLFQKYLDLTKAVSHFEKAISLNPKDAEAHIFLWYAKVYTKERKVNLQDPLL
                     IRSFELNPNLFINNYLVGNSLNKEKRYKESLPYLEKALKISPRNMNSMITLSSDYMNL
                     NQMDKAKQMLESAEKIGPSNFYVCINYGAYYAKQKELDKALEYYGKASYIKTDNAFPL
                     KAMAILYKNKGESAKAKEYFQKACELSDRESCTEAGKN"
     gene            complement(942..1325)
                     /locus_tag="PK683_15310"
     CDS             complement(942..1325)
                     /locus_tag="PK683_15310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH09862.1"
                     /translation="MQTLRGSSLMAMQVKKSKENIIVYLSGNMDNKSAHELEMEFEDL
                     LNANSDKNLILNMKEVKYLSSAGLRTIVALRSAMHESGKSLRLCHLKRNVMEVFELTK
                     VSQFFKIYEDEISALSEQEDDEDED"
     gene            complement(1298..>1606)
                     /locus_tag="PK683_15315"
     CDS             complement(1298..>1606)
                     /locus_tag="PK683_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNH09863.1"
                     /translation="VVQKALEEARQKGKIGKSLEAAVELEKAPFLQDAGSEKLEMIFT
                     VSQVRFKQTEGADVLSSREENGIKVNILYPKNPECPRCWRHTADTSEGKLCKRCEEAV
                     "
BASE COUNT          480 a          357 c          315 g          454 t
ORIGIN      
        1 cctgaagaag cccccaatac tgtactggga accaaaatag aagaggccta tgagttttac
       61 agaaagggaa tggaaaagtt cctcatttca gacgaaacga gcctgcttga agcagcagca
      121 tttttcagag aagcactcag aattgatcca ggatttgcga gagccagagc aaggctttcc
      181 tctgcttacc agatccttgt ttcctctgac ttcaatgacg gaaaagaaga agagtatctg
      241 actctcggct acaatgaagc attaaaggcc taccagagtg ctccaaattc ttcggaaaca
      301 aatattgcag tcggtcagtt taatcttctg tttcagaagt atctggattt gaccaaggca
      361 gtttctcatt tcgaaaaagc tatttctctg aatcctaagg atgcggaagc gcatattttt
      421 ctctggtatg caaaggtata tacaaaggaa agaaaagtta atctgcagga ccctcttctc
      481 atccgttcat tcgagctgaa tccgaatctg ttcataaaca attacctggt aggaaattca
      541 ctgaataagg aaaaaagata caaggagtct ctgccctatc ttgaaaaagc tctgaaaata
      601 tctcccagaa atatgaactc tatgattaca ttaagttcgg attatatgaa tctgaatcag
      661 atggacaagg caaaacagat gctcgaaagt gcggagaaaa taggcccaag caatttttat
      721 gtatgcatta attacggagc ctactatgca aaacagaaag aactcgacaa ggctctggaa
      781 tattacggaa aagcttccta cataaagacg gacaatgcat tccctctcaa ggctatggcc
      841 attctgtaca aaaacaaagg cgaatcggca aaagcaaaag agtatttcca gaaagcctgc
      901 gagctttcag acagagaatc atgcacggag gcggggaaga attaatcctc gtcttcgtcg
      961 tcttcctgct ctgaaagtgc agatatctca tcctcgtaaa ttttgaaaaa ctgagacacc
     1021 tttgtaagtt caaatacttc cattacattt cttttcagat gacagagacg caggcttttc
     1081 cctgattcat gcattgcact tcgaagtgct acgatagtac gaagacctgc tgaagaaaga
     1141 tatttcactt ccttcatatt cagaatcaga ttcttgtccg aatttgcatt cagcagatct
     1201 tcgaactcca tttccagctc atgagccgac ttattatcca tatttccgga aagatatacg
     1261 ataatatttt cctttgattt ctttacctgc attgccatca gactgcttcc tcgcagcgtt
     1321 tgcataattt cccctctgat gtatccgccg tgtgtctcca gcatctggga cattcaggat
     1381 tcttgggata caaaatattg acttttatgc cgttttcttc tctcgaggaa agtacatctg
     1441 ctccttctgt ctgtttgaaa cgcacttgcg aaacagtaaa gatcatttcg agtttttccg
     1501 aacctgcatc ctgtaaaaaa ggagcttttt ccagttccac ggctgcttca agagacttgc
     1561 cgatctttcc tttctgtctg gcctcttcga gggccttctg cacgac
//
LOCUS       DAOQGJ010001087         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57169_length_1605_cov_4.868387, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001087 DAOQGJ010000000
VERSION     DAOQGJ010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57169_length_1605_cov_4.868387"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PK683_15320"
     CDS             <1..1055
                     /locus_tag="PK683_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09864.1"
                     /translation="YKYKPISQRGAHEVTGKTFEKKTGGTQQEHKNAVEKALQNGNRV
                     SLHILRDYPDLIQKYGMGKRLERADKINAKVKEIMEKNQAKPQSSSAAEYNSMSEEDK
                     NAFINKLADEEKGNELLAIAAENESKISITLPDMQFQTEYQKEYVDKYRTDLTDSLKK
                     NLSFLDIFTRIPQETPEGNKQLAEIRNNLNLIRTGIEKISNPSFYIQQQKKIQQLVGN
                     TLIKMGRVQDEIQALAKAVNDESKTVLQKEKAESNRKTDFSNGIEGGYSQGINVVKVK
                     MQDGSKTYKIMSPYLPDLVNKIRSVKDYYYKMGREERKTAGIAPTFDGKSKAWYFGEG
                     LSGQLENVLRDYLHKS"
     gene            complement(1058..1516)
                     /locus_tag="PK683_15325"
     CDS             complement(1058..1516)
                     /locus_tag="PK683_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09865.1"
                     /translation="MKTTKQILGLIDSSQPRLALVLVTCNRSTRTAHIGSHDTGNYQP
                     IQTDAKAVFTLQRMKDIYSKVSKLMSIQPDAIQVHDMQFYNRETKREITDTEFKNGDY
                     DEGGYITTYKKYFYYPIENLFRIYEDGMPVYENNDGDICSDADALADCLM"
BASE COUNT          545 a          285 c          314 g          461 t
ORIGIN      
        1 tttataaata taaaccgatc agtcagaggg gtgctcatga ggtaaccgga aaaacgtttg
       61 aaaagaaaac aggtggcact cagcaagaac acaaaaacgc agtggagaaa gccctacaaa
      121 acgggaacag ggtttcactt cacatcctgc gtgactatcc cgatctgatc cagaagtatg
      181 ggatgggtaa acgtcttgaa agagcggata agataaatgc aaaagtcaaa gaaattatgg
      241 agaaaaatca ggcaaaacct caaagcagtt ctgctgctga atataattcg atgtccgaag
      301 aagacaaaaa tgctttcatt aataaacttg ctgatgaaga gaaaggaaat gaacttcttg
      361 caattgctgc cgaaaatgaa agcaaaattt caataaccct tcctgatatg cagtttcaga
      421 cagaatatca aaaggaatat gtagataaat acagaactga tcttactgat tcactgaaaa
      481 agaatctcag ttttctggat atttttacaa gaattccaca ggaaacacct gaagggaaca
      541 aacagcttgc tgaaataaga aataatctca atttaatcag aactggcatt gaaaaaatca
      601 gcaatccatc cttttacatt cagcagcaga agaaaattca gcagcttgtt ggcaatactc
      661 ttattaaaat gggacgtgtt caggatgaaa ttcaggcact ggctaaagct gtaaatgatg
      721 agtcaaaaac tgtcttgcaa aaagaaaaag cagaatcaaa ccgaaaaact gatttcagca
      781 acggtattga gggagggtat tctcagggaa tcaatgttgt gaaagtaaaa atgcaagacg
      841 ggtctaagac ttacaaaatt atgagtccat acctgccgga tcttgtaaat aaaatcagat
      901 ccgtaaaaga ctattattat aagatgggtc gagaggaaag aaagactgca gggatagctc
      961 ctacatttga cgggaaatct aaggcatggt attttgggga aggactctca ggacagctag
     1021 aaaatgtcct gagagactat ctgcataaga gctaatatta catcagacaa tcagccaaag
     1081 catcagcatc cgagcatata tcaccatcat tgttttcata tactggcata ccatcttcat
     1141 agattctgaa aaggttttct atcgggtaat aaaaatattt tttgtatgta gtaatatacc
     1201 cgccctcatc ataatcgcca tttttgaact cagtgtctgt tatttctctt tttgtctctc
     1261 tgttataaaa ctgcatatcg tgcacttgga ttgcatcagg ctgtattgac atcagctttg
     1321 atacctttga gtatatatct ttcattctct ggagtgtaaa aacagccttt gcgtctgtct
     1381 gaataggctg ataatttcca gtatcatgac tgcctatgtg agccgttctt gttgatctgt
     1441 tgcatgttac taaaactaat gccaacctag gctggctgct gtctatgagt ccgagaattt
     1501 gtttagttgt tttcatggtt tttgttctcc cttttatact ctaattttcg gttgatttcc
     1561 agaatcacat taacactttt tttctgtcag aaaaattatt tgttt
//
LOCUS       DAOQGJ010001088         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57185_length_1605_cov_2.610323, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001088 DAOQGJ010000000
VERSION     DAOQGJ010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57185_length_1605_cov_2.610323"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..>1605
                     /locus_tag="PK683_15330"
     CDS             80..>1605
                     /locus_tag="PK683_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNH09866.1"
                     /translation="MGKNARQEAIARISEYLPESSSFDPLETPVTELFGKNVFNDKVM
                     QERLPKSVYKSLKKTINDGKPLDPEVADIVANAMKDWAIENGATHYTHWFHPLTGLTA
                     EKHDSFINPTSNGSVILEFSGKELIKGEPDASSFPSGGIRATFEARGYTAWDATSPAF
                     IRDTTLCIPTAFCSYTGEALDKKTPLLRSMDVLSGQALRILRLFGNKTATRVTTTVGA
                     EQEYFMIDKNFYNLRPDLISVGRTVFGARPPKGQESEDHYFGSIKERILQCMKDIEAE
                     LWALGVSAKTRHNEVAPAQFEVAPIFTTTNLAMDHNQLVMDAMKKVANRHGLATLLHE
                     KPFKGVNGSGKHNNWSMGSNDGHNLLEPGDNPHENAQFLVFLVAVIKAVDTHAELLRV
                     AISNPGNDHRLGANEAPPAIISIFLGDQLTDIIDQLEKGTATSSKSGSHMEIGVTTLP
                     PLPKDTTDRNRTSPFAFTGNKFEFRAVPSSLSLGDPNCYLNTMVAEALDEAATVLENE
                     V"
BASE COUNT          471 a          364 c          337 g          433 t
ORIGIN      
        1 acagggaaac tgcttaaaaa ataggagcaa ttcgctgtat atgactgcaa tctgctaata
       61 ttctaagaaa ggatttacta tgggtaagaa tgccagacag gaagcaattg cgagaatttc
      121 ggagtatctt ccagaatctt ccagctttga tccgctggaa actccagtta cagagctttt
      181 cggaaaaaat gtcttcaatg acaaggtcat gcaggaaaga cttccgaaat cagtatacaa
      241 atctctgaaa aaaacaatca atgacggaaa acctctcgat cctgaggtag cagacatcgt
      301 cgccaatgca atgaaagact gggcgatcga aaacggtgct acgcattata cccactggtt
      361 ccatccatta accggtctta ctgcagagaa acatgattct ttcatcaatc ctacttccaa
      421 tggaagcgtg attcttgaat tcagcggaaa ggaactgatc aagggcgagc ctgatgcttc
      481 ttctttccct tcaggaggaa tccgtgctac ttttgaagcg agaggatata ctgcatggga
      541 tgcaacatct cctgctttca tccgcgatac tactctctgc attcctacgg ctttctgttc
      601 ctacacagga gaggctcttg acaaaaaaac tcctcttctt cgttctatgg atgttctgtc
      661 cgggcaggct ttaagaattc tcagactttt cggaaacaag actgcaacca gagtgaccac
      721 tactgtaggt gctgagcagg aatatttcat gattgataag aatttttata atctgagacc
      781 tgatcttatt tcggtaggaa gaactgtatt cggtgcaaga cctcctaaag gtcaggaatc
      841 cgaagatcat tatttcggtt ccatcaagga aagaattctt cagtgcatga aagacattga
      901 ggctgaactc tgggcactcg gcgtttctgc aaagacaaga cacaacgaag tggctcctgc
      961 acagtttgaa gttgctccga ttttcactac aacaaatctt gcgatggatc ataaccagct
     1021 tgtaatggat gcaatgaaaa aagtcgcaaa ccgtcacggt cttgcaactc ttcttcatga
     1081 aaaacctttc aagggagtca acggatcagg aaagcacaac aactggtcaa tgggttccaa
     1141 tgacggtcat aatcttcttg agcctggaga caatccgcat gaaaatgcac agttccttgt
     1201 attcctggtt gcagtgatta aggcagtgga tactcatgct gaacttctgc gtgttgctat
     1261 ttccaaccct ggaaacgatc acagactcgg tgcaaacgag gctcctccag caattatttc
     1321 catctttcta ggagatcagc tgactgacat catcgatcag cttgaaaaag gaactgccac
     1381 ttcttccaaa tcaggatctc atatggaaat cggtgtgact actcttcctc ctcttcctaa
     1441 agatacaaca gacagaaaca gaacttcccc gtttgcattc actggaaaca agtttgaatt
     1501 ccgtgctgtt ccttcctcac tgtctctcgg tgatcctaac tgttatctca atacaatggt
     1561 tgctgaagca cttgatgaag ctgcaactgt tcttgagaat gaggt
//
LOCUS       DAOQGJ010001089         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57189_length_1605_cov_2.441290, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001089 DAOQGJ010000000
VERSION     DAOQGJ010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57189_length_1605_cov_2.441290"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PK683_15335"
     CDS             <1..1170
                     /locus_tag="PK683_15335"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNH09867.1"
                     /translation="RSLVFSVLWGIPLIVILVLPWAWWVNQETAGKFLEEFVIKHNVQ
                     RGLGGDEQLDGHLHPWWYYLLRIWLDTAPWGLLLPVCVTALIQRKLTSRWTITGLAWF
                     LSILIFLNILFGPLVRATNSGLACPDWPLCYGKVIPPAEFRIWMEVGHRIYSAILSIL
                     FVYMLVMIFRSELLRKSYAFLGISAAAVLIFQIALGALTVTKLLDPGTVNSHLLNAVL
                     FLLLNVTVFLKSRVQIGSPHFAGIGFAEIFSKENIMLKIGFVLIYVQLFMGGRVSSNY
                     AGMACIDWPMCNGKWFPEMKGLIRYQMEHRYLAYIIMAFLIILFIQAFHKKHNTLYKR
                     LCTLMLYIGGVQIAAGVFNVLWSLPILLTAFHTGNGVMLLTVMYVTYYVKESRLG"
     gene            1167..>1605
                     /locus_tag="PK683_15340"
     CDS             1167..>1605
                     /locus_tag="PK683_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HNH09868.1"
                     /translation="MKHWLLVWLKLIKPRVTSLVLVTALPGLYLGLENISGSLVFWTL
                     FGTFLMSSASFVFNQVIEKDRDAQMDRTKNRPIPKGDISPAGAFAGGILLVIASFAVF
                     YLKVNLLSFYCAFAALIAYVFLYTIYLKPRTEQNIVIGGVAGCV"
BASE COUNT          372 a          370 c          362 g          501 t
ORIGIN      
        1 cgttcgctgg tcttctccgt tctttgggga atcccactga tcgttattct ggtactcccc
       61 tgggcctggt gggtcaatca ggaaactgct ggtaaatttc tcgaagagtt tgttatcaaa
      121 cacaatgtac aacgaggact tggtggtgat gaacaacttg atggccacct gcatccctgg
      181 tggtattacc tcctacgcat ctggctggat actgctccgt ggggattatt gttgcccgtt
      241 tgtgtcactg cattgatcca acgcaagctc acttctcgct ggacaattac cgggctggca
      301 tggtttctct ccattctgat ttttctgaat attcttttcg gtccgcttgt gcgtgctact
      361 aattccggtc ttgcctgtcc ggactggccg ctgtgctacg gaaaggtcat tcctcctgct
      421 gagttcagaa tctggatgga agtgggacac agaatctatt ctgcaattct ttcaattctg
      481 tttgtatata tgctcgtaat gatattccgg tcggaacttc tcagaaaatc ctatgctttt
      541 cttggaattt cagccgctgc tgttcttatc ttccagattg cactcggtgc actgactgtc
      601 acaaaacttc tcgatccggg aacagtcaat tctcacctcc tgaatgcagt tctgttcctc
      661 cttctgaatg tcacagtttt cctgaagtcc agagtgcaga tcggttctcc tcattttgcg
      721 ggaattggat tcgctgagat attttctaaa gaaaatatca tgctgaaaat cggatttgtc
      781 ctcatttatg ttcagctctt catgggagga agagtcagtt ccaattatgc gggaatggcc
      841 tgtattgact ggccgatgtg caacggaaaa tggttccctg aaatgaaagg tctcatccgg
      901 taccagatgg agcacaggta ccttgcctac attattatgg ctttccttat tattcttttc
      961 attcaggcat ttcataaaaa acacaataca ctgtataaaa gactctgcac tctgatgctg
     1021 tatatcggag gagtgcagat tgcagcagga gtattcaatg tgctctggtc cctcccaatc
     1081 cttctcacag catttcatac agggaacggt gtgatgcttc tgactgtgat gtatgtgaca
     1141 tactacgtaa aagaaagcag gctgggatga aacactggct tttagtatgg ctgaaactca
     1201 tcaagcctag agtcacctct cttgtgcttg tcactgcact tcccggactg tatctggggc
     1261 tggaaaatat ttccggaagt ctggtttttt ggacactgtt cggaacattc ctgatgtctt
     1321 ctgcatcctt tgtattcaac caggtgattg aaaaagacag ggatgcacag atggacagaa
     1381 caaaaaacag accgatcccg aaaggcgata tttcccctgc aggcgcattt gcaggcggga
     1441 ttctgcttgt catcgcctca tttgcagtat tctatctgaa agtgaatctt ctctccttct
     1501 actgtgcatt tgccgccctg atcgcatatg tttttttata tacaatatat ctcaagccga
     1561 gaactgaaca gaacattgtc atcggaggtg ttgcaggctg tgtcg
//
LOCUS       DAOQGJ010001090         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57204_length_1605_cov_1.631613, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001090 DAOQGJ010000000
VERSION     DAOQGJ010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57204_length_1605_cov_1.631613"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK683_15345"
     CDS             <1..350
                     /locus_tag="PK683_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH09869.1"
                     /translation="GAGKVSENKFIFSFEPPIGEMNRDYYLNQFRWVSGENVNLRLYP
                     DRDVIEKISYEFVKFLEYDKKNPDWEKVQTYDNKTGYINVKYLQSFESGNKRLIFSKI
                     NGKWKIIAFIGGI"
     gene            505..1110
                     /locus_tag="PK683_15350"
     CDS             505..1110
                     /locus_tag="PK683_15350"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNH09870.1"
                     /translation="MRERPGIKYKKLSTLPFGSEVKILEYSKEENTVNRRTDRWAKIK
                     TDDSAGWVFNAYLIKEIPQNSFQRIPNKKIESFLRNYKFGKLLNSKKIGRDECNGASV
                     TKKEYNGNAIIISMEQHRYDYLFKDSFEDVLGISNGYTKEQVLKILGNPYSSEKNELE
                     YISEKPEGYESEYFESRWIIKIFLNKNKVNQIRMMELFDDC"
     gene            complement(1406..>1605)
                     /locus_tag="PK683_15355"
     CDS             complement(1406..>1605)
                     /locus_tag="PK683_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09871.1"
                     /translation="GIQQAFQTQALLGIISGQSMWVFGYNGCFSKATWTERKNHIMVF
                     SVRTQSPIQEEKKVSICKESK"
BASE COUNT          577 a          232 c          319 g          477 t
ORIGIN      
        1 ttggagcagg aaaagtttct gaaaataaat ttatattttc ctttgaacct ccaatcggag
       61 aaatgaatcg agactattat ctcaatcaat tcagatgggt gagcggagaa aatgtaaatc
      121 tcagattgta tccggataga gatgtcattg aaaaaatatc ttatgaattt gttaaatttt
      181 tggagtatga taaaaaaaat cctgattggg aaaaagttca gacatacgac aataaaaccg
      241 gatacataaa tgtaaaatat cttcagtcat ttgagtctgg aaacaaaaga ctgatatttt
      301 ccaagataaa cggaaaatgg aagattattg cttttatcgg aggaatatga ttcactcgct
      361 tacggtttac agtctgacag ggagaggatt aaaaagatta tgacggcttt gaaattatca
      421 gtaatgatat tctctttatt tgccttcagc agtataatcg ctgatgataa tactatgtat
      481 gtaaatgcga aaagaggctt gattgtgaga gagcggccag gaataaaata caaaaaattg
      541 agcactctgc cctttggttc ggaggtgaaa attctggaat attcaaaaga agaaaatact
      601 gtcaatcgca gaacagacag gtgggcaaaa ataaaaacag atgattctgc cggctgggta
      661 tttaatgcct atcttatcaa agagattcct caaaattcat ttcaaagaat accgaataaa
      721 aaaatcgaat ccttcttaag gaattataaa tttggaaaac tgttgaattc aaaaaagatt
      781 ggtagagatg aatgcaatgg agcatctgta acaaaaaaag aatacaatgg caatgcgatt
      841 ataatatcta tggagcagca tcgatacgat tatttattta aagacagttt tgaagatgta
      901 ttaggaattt ctaatggata tacaaaagag caggttctga aaattctcgg gaatccctat
      961 tcatctgaga agaatgagct ggaatacata tcggaaaaac cggaaggata tgaatctgag
     1021 tattttgaat cccgctggat aataaaaata tttttgaaca aaaacaaggt gaatcagatt
     1081 cggatgatgg agttgtttga tgactgctga ttttaaaata caatggataa atgcaaaaag
     1141 acgactgaca agtcctgcgt attctgaatt cttccttaac tgtaatgctg acatcaggat
     1201 ggtttcaaaa agcttcggag gttttggtgt ggtccggaaa ttgaagcgaa taataaactg
     1261 aaaaagtatg atcccaggag caggttcaat gcagaaagat aaaaagaaaa cagtttttaa
     1321 tgagtcgttc tattggaaag gacagagatt tatatacttc attgcatttc cgatattgac
     1381 atttgcagca gcacttactc agggttcatt tggattcctt gcatatggaa actttttttt
     1441 cttcttgtat gggggattga gttcttacac taaataccat tatatgattt tttctttctg
     1501 tccaagttgc ttttgaaaaa caaccgttat aaccaaacac ccacatcgat tgtccagaaa
     1561 ttatacctaa caatgcctgt gtttggaaag cctgctgtat accct
//
LOCUS       DAOQGJ010001091         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57213_length_1604_cov_4.013557, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001091 DAOQGJ010000000
VERSION     DAOQGJ010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57213_length_1604_cov_4.013557"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1604
                     /locus_tag="PK683_15360"
     CDS             <1..>1604
                     /locus_tag="PK683_15360"
                     /inference="COORDINATES: protein motif:HMM:NF023404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09872.1"
                     /translation="SYTDKETGKSYKEGDEIFITGKDQMIYFPRPEHAIIKYGVSDTH
                     YAVAIPSGEGRYILNRLTGVVSLIKGPAMYLPNPMHEVIVRRILDDHVVRLWFPGNKE
                     AVEVNAILKAEVEKNKPSRAAAAPAAEVWQQVDSLLAKAESADDAEEGFSGDSFERNP
                     VYTPPRTITLDTKYEGAVAINVWTGYAVMVISKTRDRKVIVGPQTYLLEYDETLDVAS
                     FSKGVPKSDKECERTVYLRILNNRVSDLVEAETRDYCKIAVKTALKVDFEGDPSRWFN
                     VENYVKFLTDRFRSVIRSEVKKYNLQAFYDDAAGLMKDMLLGKEVNGARPGYRFEENG
                     MVVKDLEILQLMIIDQKIATALINSRHEEIQQSLVLEKDKRELLYTKENEIIRREKET
                     VTLATQQMILGLKEDFIKKQIEYEELAFKLESDLAAKKRDNELKSEKEQNEIEAARRQ
                     REREAEELALKIKSANQKVELEYLKAEVAALASKAEAITPEFIAALQAFGDKDLLLKM
                     AESMAPLSILGGKSVSEVFANLLKGT"
BASE COUNT          503 a          314 c          434 g          353 t
ORIGIN      
        1 actcttatac ggacaaggag actggaaaat cctacaagga gggggatgag attttcatca
       61 caggaaagga tcagatgatc tatttcccga gaccggaaca tgccattata aaatacggcg
      121 taagcgatac tcattatgct gttgcgattc cgagcggcga gggccgttac atactgaaca
      181 ggctgaccgg tgttgtatcc ctgatcaaag gccctgcaat gtatctgccg aacccgatgc
      241 atgaggtcat tgtcagaagg attctggatg atcatgtggt gagactctgg tttcccggaa
      301 acaaggaagc agttgaagtc aatgcaattc tgaaagctga agtggaaaag aacaagccta
      361 gcagagcagc agcggctcct gcggcagagg tctggcagca ggtggattcc cttcttgcaa
      421 aggcagagtc agcagatgat gcagaggaag gtttttcggg agattctttt gaaagaaatc
      481 ctgtgtacac tcctcccaga acaatcactc tggatacaaa gtatgagggg gcagtggcaa
      541 tcaatgtctg gacgggatat gctgtaatgg tcatcagcaa gacccgggac aggaaagtga
      601 ttgtaggccc tcagacatat cttctggagt atgatgaaac tctggatgtg gcatcctttt
      661 ccaagggagt tcccaagtcg gacaaggaat gtgaaaggac tgtctatctg agaattctga
      721 acaacagggt atctgacctt gtggaagcgg agacaaggga ctactgcaag atcgcagtca
      781 agactgcact gaaagtggac tttgaaggag atcccagccg atggttcaat gtggagaatt
      841 atgtaaagtt ccttacggac aggttcagat cagtgatccg ttccgaagtg aaaaaataca
      901 atctccaggc gttttacgat gatgctgcag ggctgatgaa agatatgctt ctgggcaagg
      961 aagtgaacgg tgcaaggcct gggtacaggt tcgaagagaa cggaatggtc gtgaaggatc
     1021 tggagattct gcagctgatg atcattgatc agaaaatcgc aacagctctc atcaattcca
     1081 gacatgagga aattcagcag tctcttgttc tggaaaaaga caaaagagaa cttctgtata
     1141 ccaaggaaaa tgaaatcatc aggagagaaa aagagacagt cactctggcg actcagcaga
     1201 tgatcctggg tctgaaagaa gacttcatca agaagcagat tgaatatgag gaacttgcat
     1261 tcaaactgga gtccgatctt gctgcaaaga aaagagacaa tgaactgaag agtgaaaaag
     1321 agcagaacga aattgaagca gccagaagac agagagaaag agaagctgaa gaacttgcac
     1381 tcaagataaa gtctgcaaat cagaaagttg aactggagta tctgaaggca gaagtggctg
     1441 ctcttgcatc caaggcggaa gcaatcactc cggaattcat tgcggccctt caggcattcg
     1501 gcgacaagga tcttcttctc aagatggcgg aatccatggc tcctctttcc atcctgggtg
     1561 gaaagtcagt cagcgaagta tttgcaaatc ttctgaaagg aaca
//
LOCUS       DAOQGJ010001092         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57253_length_1604_cov_2.367979, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001092 DAOQGJ010000000
VERSION     DAOQGJ010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57253_length_1604_cov_2.367979"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK683_15365"
     CDS             complement(<1..257)
                     /locus_tag="PK683_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09873.1"
                     /translation="MFTLYITILFFFLMLTLSVTDRMIFFETGPMIPFFSIPMSLQPF
                     CLLFPFPVTLMHFLVLQRNTEQNLFMKFLSTEGHDIKKIDRV"
     gene            complement(262..>1604)
                     /locus_tag="PK683_15370"
     CDS             complement(262..>1604)
                     /locus_tag="PK683_15370"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNH09874.1"
                     /translation="SKKRYEFSIFLSILFFLHCSHSANEPYSGGAGTSFDFSLNAFNR
                     HIANLKDNDKIMQFNRGNQIFKAVWSNDGGPVFQGLGPLFNSRSCNTCHFLGGRGRNR
                     LDSEKEFYFLIFRVRNGSGAALENYGTQIQDRSVSKAIPPEAVPDVKYSEIPGTFQDG
                     SKYSLMKPEYKIIQWNYGNPSEYHLSPRVPPAVFGMGLLSQISDETLKSFEDPEDRNG
                     DGISGRISKVKNRLTSKEETGRFGWKASQPDVLHITANALHQDMGITSELFPNQNCEP
                     VQSGCTKTPFVSPEIDGTTLKDLVYSVMMTAVPGRRNLQTQEGKKIFLEIGCGDCHKS
                     EMRTDTHPKFPELSNQTIYPYTDLLLHDMGKELADQIREGSAEGNEWRTPPLWGLGLY
                     SLVNQHSFLLHDGRARNAEEAVLWHGGEAEKSRDKYRNLRRSDREKILDFLYSL"
BASE COUNT          427 a          337 c          343 g          497 t
ORIGIN      
        1 actctgtcga ttttttttat gtcatgtcct tcggtgctga gaaatttcat aaaaagattc
       61 tgttcagtgt ttctttgaag aacgaggaaa tgcatcagtg ttacagggaa aggaaaaagc
      121 agacagaaag gctgcagact catagggatg ctgaaaaaag gaatcatcgg tcctgtttcg
      181 aagaagatca ttctgtccgt gacagacagt gtcagcatca ggaaaaagaa aagtattgtt
      241 atatataatg taaacatatg attatagtga atataggaaa tcgagaattt tctcacgatc
      301 agatcttctc agatttctgt atttgtctct ggacttttct gcttctccgc cgtgccagag
      361 aactgcctct tcggcatttc ttgccctgcc gtcgtgcaga aggaaactgt gctgatttac
      421 aagagaatac agtccaagac cccaaagcgg cggagttctc cactcatttc cttccgcact
      481 gccttccctg atctgatctg caagctcttt tcccatatcg tgaagaagaa gatccgtata
      541 aggataaatt gtctgattcg aaagttcagg aaatttgggg tgagtatcag ttctcatttc
      601 tgatttatga cagtctccgc atccgatttc aagaaatatt tttttccctt cctgagtctg
      661 cagatttctt cttccaggga ctgctgtcat cattacagaa tataccagat ccttcagtgt
      721 tgttccgtct atttccggtg agacaaatgg ggttttggta cagcctgact gaacaggttc
      781 gcagttctga ttcggaaaaa gttcagatgt gattcccata tcctgatgaa gggcattggc
      841 tgtgatgtga agaacatccg gctgtgacgc tttccatccg aatctgcctg tttcttcttt
      901 gctggtcagc ctgtttttca cttttgaaat ccgtccggaa atcccgtctc cgtttctgtc
      961 ttccggatct tcaaaagatt ttaatgtttc atccgaaatc tgactcagta gtcccatacc
     1021 gaatactgca ggaggaactc tcggggaaag atgatattcg gaaggattcc cgtagttcca
     1081 ttgaatgatt ttatattccg gtttcattaa agaatatttt gagccgtcct ggaatgttcc
     1141 tggaatttca gagtatttta catctggaac cgcttctgga ggaattgcct tgctgacaga
     1201 tctgtcctga atctgagttc catagttttc tagggcagca cctgaaccgt tcctgactcg
     1261 gaaaatcaga aaatagaatt ctttttctga atccagacga tttcttcccc ggcccccgag
     1321 aaaatggcag gtattgcagg atctggaatt gaataaaggc ccaagaccct gaaatacagg
     1381 ccctccgtca tttgaccata ctgctttgaa tatctgattt cctctgttga actgcatgat
     1441 cttatcattg tctttcaggt tggcgatgtg cctgttgaac gcattcagag agaaatcaaa
     1501 actagtgccc gcaccgcctg aatagggttc atttgccgag tgagaacagt gtaaaaaaaa
     1561 caatatggaa agaaagattg agaattcata tcttttttta ctgt
//
LOCUS       DAOQGJ010001093         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57287_length_1603_cov_3.160853, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001093 DAOQGJ010000000
VERSION     DAOQGJ010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57287_length_1603_cov_3.160853"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PK683_15375"
     CDS             complement(<1..908)
                     /locus_tag="PK683_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH09875.1"
                     /translation="MIKKIIRFSAENKFLILALTVILILSAVLSMKRIRLDAIPDLSD
                     TQVILYTKWDRSPDLIEDQITYPIISSLLGAPKVKAIRGFSDFGYSFVYVIFQDGTDI
                     YWARSRVQEYVSRVQSSLPAGAKLEMGPDATGVGWVYQYALLDRKNSMSPYELRALQD
                     FKLRFLLNSIPGVAEVTSIGGYRKQYQIEVSPEAMQAYNISMNDILSKVSASNDETGA
                     RVMEIAGREFMIRGRGYVKDKSDIEKVSLGTDSKGYPVLLKNIARVSIGPEMRRGVGE
                     FDGIGETPGAIIVMRQGENALQVISAV"
     gene            complement(901..>1603)
                     /locus_tag="PK683_15380"
     CDS             complement(901..>1603)
                     /locus_tag="PK683_15380"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH09876.1"
                     /translation="KSPLSKVIYSAGTVSYEPDIYSAVLEMQIGGAFSSSARLKLEQM
                     GVSESFIREWVSRDPKELIVGGKGTAHIVSVLYENEFSSVKTGSSVQVTSASLPGRIF
                     KGTVRHIDRIPDAKTRTLKVWTDVKDAENLLKPQMFVKTEISSGTGEGLTVPVSSVID
                     TGERQIVYVRTGKGKFSQRNVRTGVEAGDVLEIKEGLSEGEEIAEYGIFFIDSESRLK
                     NSEETPKKEQEHSHD"
BASE COUNT          396 a          448 c          301 g          458 t
ORIGIN      
        1 actgcggaaa tgacctgaag agcattttct ccctgcctca tcacaatgat tgcacccgga
       61 gtttcaccga tcccgtcaaa ctcgcccact ccccttctca tttcaggacc tatgctcacc
      121 cttgcaatat ttttcagaag gacaggatat cccttgctgt ctgttccgag actcactttt
      181 tcaatgtccg atttatcctt cacataaccc cttccccgga tcataaactc ccgtcctgca
      241 atctccatca cccttgcacc tgtctcatca ttggatgcag acaccttgga tagaatatca
      301 ttcatggata tattatatgc ctgcatagct tcaggactca cttcgatctg gtactgtttt
      361 ctgtatcctc caatggaagt gacttcagcc actccgggaa tgctgttcag aagaaaccgg
      421 agcttgaaat cctgaagggc cctcagttca taaggagaca tactgttttt cctgtccaga
      481 agagcatact ggtacaccca gccgactccc gttgcatcag gtcccatttc aagcttcgca
      541 ccggcaggaa gactcgactg caccctggat acatattcct gaaccctcga tcttgcccag
      601 tagatgtccg tcccgtcctg aaatattaca tatacaaagg aatacccgaa atcggaaaaa
      661 cccctgatcg ccttgacctt gggagcgccc agaagactgg aaattatggg atatgtaatc
      721 tggtcttcaa tcagatcagg acttctgtcc catttggtat acagaatcac ctgagtgtcg
      781 gaaagatcag ggattgcatc gagcctgatt cttttcatgg aaagcactgc ggacagaatc
      841 agtatcactg tcagtgcaag gatcagaaat ttattctctg cagaaaaccg gatgattttc
      901 ttaatcatgg ctgtgctcct gctccttctt gggagtctct tcagaatttt tcagtctgga
      961 ttcagaatcg atgaagaata tcccgtactc agcaatttcc tctccttctg acagtccttc
     1021 cttgatttcc agaacgtcac ctgcctccac gcctgtcctg acgtttctct gggaaaactt
     1081 tccttttcct gtcctgacat acacaatctg tctttcacct gtatcaatga ctgaactcac
     1141 aggaacagtg agcccctccc ccgttccgga agaaatttcc gtttttacga acatctgagg
     1201 cttgagaaga ttttctgcat ccttcacatc tgtccagact ttcagagtcc tagtctttgc
     1261 atccggtatc ctgtcaatat gtctgactgt gcccttgaat atcctgcccg gaagagaggc
     1321 tgatgtcacc tgaacggaac tgcctgtctt gacggaagaa aattcatttt catataacac
     1381 agatacaata tgtgcagttc cttttcctcc cactatcagc tcctttggat ctctcgaaac
     1441 ccattccctg ataaaacttt ccgacacccc catctgctcg agcttgagcc ttgccgagga
     1501 ggaaaatgcc cctcctatct gcatttccag aactgcagaa tatatatccg gttcataact
     1561 gactgttcct gcagagtata tcaccttgga caatggactc ttc
//
LOCUS       DAOQGJ010001094         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57290_length_1603_cov_2.995478, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001094 DAOQGJ010000000
VERSION     DAOQGJ010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57290_length_1603_cov_2.995478"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PK683_15385"
     CDS             complement(<1..719)
                     /locus_tag="PK683_15385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH09877.1"
                     /translation="MRVIFRIGFLSALISFSAFPEEKKTDKIEVIGNKESAAGSAKGT
                     KTDPGGFVDVIKSESFKDRYVSLPDVLEREAGVRVRRFGGLGSYSTLSIRGSNPNQVQ
                     IYIDGVPLSNAQGGEINLSDLNFDNLDRIEIHKSGSASGMSSSSIGGSVNLITKKTDV
                     SRKTRFSVSGGSFNTFKITGSKSGEYKDVKYSIFGQKEKSDQNFTFRSDNGTPVLNTF
                     DDFDTKRRNAQFDRYSLTGTLA"
     gene            856..>1603
                     /locus_tag="PK683_15390"
     CDS             856..>1603
                     /locus_tag="PK683_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNH09878.1"
                     /translation="MLRGLKRLNRYEKRLIEIAKKGGSLVRISQFGFSSKTEQGFRFP
                     IYSLEIGKEKAVRKNPAGLIAGVHGLETIGIRVLLDFLEFVLEDGASPILKDLKKGEF
                     GIVCIPILNPGGVAMKRRSNPRGVDLMRNSGLKAEEAPFFFGGQSFSSKLPYYQGKTL
                     EAESRVLYRFAKEYFFQPDKSIIPVLDIHSGFGMTDHIWWPYAKSQEKCPDDALYMKL
                     AQYLKEEKNHQSYKFGPQSETYTINGDLWDR"
BASE COUNT          447 a          348 c          340 g          468 t
ORIGIN      
        1 gcaagagttc ctgtcagact gtacctgtcg aactgggcat ttcttctttt tgtatcaaaa
       61 tcatcaaatg tattcagaac gggcgttcca ttgtccgagc ggaatgtaaa gttctgatcc
      121 gatttttcct tctgaccgaa tatactgtat ttcacatcct tgtattcacc gcttttggaa
      181 cctgtaatct tgaatgtatt gaagcttcct cctgaaacac tgaaacgcgt ttttctgctg
      241 acgtcggtct tcttcgtaat caggttgacg cttcctccga tggacgatga ggacattccg
      301 gaagcggagc cgcttttgtg gatttcgatt ctgtccagat tgtcaaagtt cagatcggag
      361 agattgatct ctcccccctg tgcattgctc aaaggcaccc cgtcaatata aatctgcacc
      421 tgatttggat tcgaccctcg aatggaaaga gtggaataag aacctagtcc gccgaacctt
      481 ctgacccgga cccctgcttc tctttccagc acatcaggaa gactgacata tctgtccttg
      541 aaggattcac ttttgatcac atctacaaat ccccctggat cagtcttcgt tcctttcgcg
      601 cttccagctg ccgattcttt gtttccgata acttcaatct tatctgtttt cttttcttcc
      661 ggaaacgcag agaatgaaat caatgcagac agaaatccaa tccggaaaat gactctcaca
      721 cgaacaaagt ttcagacggg cttttggagt aatttccttt ttgtttcatt ttgtaatcta
      781 attttaacaa ttctgcaaaa atccgctctc taaaaaagtt gtctccattc tgtaaaaata
      841 agtttctgaa tccgtatgct tcgcggatta aaacgtctca acagatatga aaaaagactg
      901 atcgaaattg caaaaaaggg cggcagtctt gtccggatca gtcagtttgg attctcatca
      961 aaaacagaac agggttttcg atttccgatt tattcccttg agatcggcaa agaaaaagca
     1021 gtacgaaaga atcctgcagg actgattgca ggtgtgcatg gactcgaaac aatcggaatc
     1081 agagtgcttc tggattttct ggaatttgtt ctcgaggatg gagccagtcc tattctgaag
     1141 gatctgaaaa aaggagaatt cggaattgta tgtataccta tattaaatcc gggaggggtg
     1201 gcaatgaaaa gaagatccaa tcctcgtgga gtggatctga tgaggaattc gggtctcaag
     1261 gcagaagaag cacctttctt tttcggagga cagagtttct ccagtaagct tccttattat
     1321 cagggaaaaa cactggaagc tgaatccagg gttctctaca gatttgcgaa ggaatatttt
     1381 ttccagccgg ataaatccat tattcctgtt ctggacatcc attccggttt cggaatgaca
     1441 gatcatatct ggtggcctta tgcaaagtct caggaaaaat gtcccgacga tgcactgtat
     1501 atgaaactgg cacagtacct gaaggaagag aaaaatcacc agtcttataa gttcggtcct
     1561 caaagtgaaa catatacaat caacggtgat ttatgggaca ggc
//
LOCUS       DAOQGJ010001095         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57393_length_1601_cov_3.958603, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001095 DAOQGJ010000000
VERSION     DAOQGJ010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57393_length_1601_cov_3.958603"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(447..929)
                     /locus_tag="PK683_15395"
     CDS             complement(447..929)
                     /locus_tag="PK683_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09879.1"
                     /translation="MLYPNIRRLQEAETLLAEGLGLISLRPGHHIIDGKKAEVSRIWC
                     CAFDYSASRLPVVSVATLKMDKTDSTLYRSALVYEQDEIAEKAHSKNLPSDPLEWFFH
                     DPIIIDAAKSGGLDGVRIDLFIADFFGPTFNISFDNAADFHLKKLQSTLLDFLNSYGN
                     "
     gene            complement(1043..>1601)
                     /locus_tag="PK683_15400"
     CDS             complement(1043..>1601)
                     /locus_tag="PK683_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09880.1"
                     /translation="NVSVFICLFLLYFCQTQSGNQSNFSKLERSFQRNKIRGFILERA
                     DWMAAGKYLGGCVGYECEQPDESVQYTFISQFAKSEELNELLYHPSGLVRCYALDALM
                     EKGKFNYYQVLKEKLSDESEVLEAHGCEVFPIKVRDYYYNRLSDKISKSEKKEIDLLI
                     LNSRNSLDVKKSLSEEKHKPAGRKK"
BASE COUNT          440 a          351 c          311 g          499 t
ORIGIN      
        1 tgcacatagc accagaaacc gtggtgattt ccggcagatc gtacactgac atagtgcgga
       61 ccagtcggga cttcactgat gcctctgaac cctccctgaa tggagaatct ggcaagatct
      121 atcttgactt cttcttttgg aatgtctctt agaattatga ttccagattg catatatgct
      181 gatccaaatt ctgtcaggtc tcggattaat gtcgagtatt ttatcttttc cgaataaaaa
      241 accctgttca gttgagcgtt tctctgaaat gcttcgctta taattcacct tacgaaggtg
      301 cttttgcaag ctttgaaact gccaagggct ttatcctctc caagcttcat tcaagatgac
      361 tgccggttta gaagctggca aggaatcctg caagcatgga ggctgctgtt ttcataattc
      421 gtttttcttc cccatactga gtatgtttaa tttccgtagc tgttcaggaa atccagcagg
      481 gtgctctgaa gttttttcag atgaaagtcg gcggcattgt caaaggaaat attgaaggta
      541 ggaccgaaaa aatctgcgat aaacagatca attctgactc cgtcaagacc gccggatttg
      601 gcggcatcta taatgatagg atcatgaaaa aaccattcca aaggatcaga aggaaggttc
      661 ttcgaatgag ccttttctgc aatctcatcc tgttcataga ccagtgcaga cctgtaaaga
      721 gtgctgtccg ttttatccat tttcagagtg gctacactga cgacaggaag tctggatgca
      781 gaataatcaa atgcacagca ccagattctg gaaacctctg ctttttttcc gtcaatgata
      841 tgatggccgg gtctgagcga aatcagtccc agtccctccg caagtaaagt ttccgcttcc
      901 tgcagtcttc tgatattcgg atataacaaa ttttcgctcc tctgattcca aaatctgctc
      961 tgttcagcaa aaaatcagac cattcttcaa tgaagcagac tcagctgttc tgaatatcga
     1021 catttatcct ctctgatgat tcttattttt ttctgcccgc tggtttatgt ttttcttcag
     1081 acagagattt tttaacatca agactgttcc tggaattcag aatcagcaaa tctatttctt
     1141 ttttttcaga tttggaaatc ttatccgaaa gtctgttata ataatagtct cttactttga
     1201 tagggaatac ttcgcagcca tgtgcttcca gaacttcaga ttcgtcagac agtttttctt
     1261 ttaagacttg atagtaatta aacttacctt tctccataag agcgtccaat gcataacatc
     1321 tgaccaagcc ggaaggatga tacaataatt cattcagttc ctcggatttt gcaaactgag
     1381 aaataaatgt gtattggaca gattcatccg gctgctcaca ctcataaccg acgcatcccc
     1441 ccaggtattt tccagcagcc atccagtcag ctctttccag aataaatcct ctgattttat
     1501 tcctttggaa tgaacgttcc agttttgaga aattactctg atttcctgac tgagtttgac
     1561 agaaatacag caggaagagg cagatgaata cggaaacatt g
//
LOCUS       DAOQGJ010001096         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57544_length_1599_cov_2.259067, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001096 DAOQGJ010000000
VERSION     DAOQGJ010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57544_length_1599_cov_2.259067"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="PK683_15405"
     CDS             <1..1436
                     /locus_tag="PK683_15405"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH09881.1"
                     /translation="LTGDIRDTYLSVYLNSPEIDRMEKLTVRQYDIFRFFIYLSYLIV
                     GVQLLVVYFYNRQKMYLFLSFGVFCYGMNRFLDSSLAFQLIEDSSVQQNLYSVTYLLL
                     SGFMLLFFNYLVEEKAHFIVKLNFFFSLFFSVMKFFLPHAYTFPFLLGFASSASLIAY
                     PYIMFYRIGYTGIKFFITELKENLRKKMKSAFLHSLFYAWKSNQEFMLFVFIFPTYIS
                     FAIDYNDFSKYEAHRYTSDFSIFLFYGLMTYFTFIRKSQLTEKHRIELEKLEWEKETE
                     KLKSELKVKKEREKIFADIHDNLGGKLLDLSFQLNSLPTDEPISPKSKDRISGSISTV
                     MKGLRNHLLAFEDMKIIEEHFAEGLQLFLIRRYSLVSRKIYFVTEVGYNDGIISRDKL
                     PDLLKIITELVNNDLKYGFGISEWRLKAEDSRFSIEMNSETHWNENRKSAGNGHITMA
                     RRTETLGAVFEYTIRNSIYSAKISFPL"
BASE COUNT          501 a          309 c          321 g          468 t
ORIGIN      
        1 gtctgacagg agacatacgg gatacttacc tgtcagtata cctgaactct cctgaaattg
       61 accggatgga aaaactgaca gtcaggcaat atgatatatt tcgttttttt atctatttat
      121 catacctgat tgtcggagtt cagctcctgg ttgtgtattt ttacaacaga cagaaaatgt
      181 atttatttct gagctttgga gtcttctgct acgggatgaa cagatttctg gacagcagtc
      241 ttgcatttca gctgattgaa gactcctccg tgcagcagaa cctttattca gtgacctatc
      301 tgcttctttc cggattcatg cttctgttct ttaattattt agtggaggaa aaagcgcatt
      361 tcatcgtaaa actgaatttt ttcttttcac tgtttttttc cgtaatgaaa ttctttctgc
      421 ctcatgccta tacatttcct tttctgctcg gattcgcctc aagtgcctct ctgattgctt
      481 atccttatat aatgttctac agaataggat atacgggaat aaaatttttc attacagaat
      541 tgaaagaaaa tctcagaaag aaaatgaaat ccgcgtttct gcattccctt ttttatgcct
      601 ggaagtcaaa tcaggaattt atgcttttcg tatttatttt tccgacatat atttcctttg
      661 caatagatta taatgatttt tccaaatatg aagctcacag atacacctca gacttttcaa
      721 ttttcctgtt ttacggcctg atgacatact ttacattcat aagaaagtcc cagctgacgg
      781 aaaagcacag aatagaactg gaaaagctgg aatgggaaaa agaaacggaa aaactgaaaa
      841 gcgagcttaa agtcaagaag gaaagagaaa aaattttcgc agacattcac gacaatctgg
      901 gcgggaagct gcttgacctt tcctttcagc tgaacagcct gccgactgat gaaccgatca
      961 gtcccaaatc caaagacaga atcagcggaa gcatcagcac tgtaatgaag ggactcagaa
     1021 accatctgct tgctttcgaa gatatgaaaa tcatagaaga acattttgca gaaggcctgc
     1081 agcttttttt aatcagaagg tattcccttg tcagcaggaa aatatatttt gttacggaag
     1141 tcggttacaa tgacggaatc atctcaaggg ataaactgcc tgacctgctg aagataatta
     1201 cagaacttgt aaacaatgat ttgaaatacg gatttggaat atcagaatgg agactgaagg
     1261 cagaagacag ccggttctcc attgaaatga attcagaaac tcactggaat gagaatagga
     1321 aatctgcagg gaacggtcat atcacaatgg ccagaagaac cgaaacctta ggtgctgttt
     1381 ttgagtatac aatccggaac agcatatact ctgcaaagat ttcgtttcct ctgtaaggtt
     1441 tttctgcgaa atgggaatac ctgaataaaa aactgctgat ttacagagaa tatactttga
     1501 tagaatttct aaagaacgca gcggcccagg atgtcgggaa ctgcaggcca agtccagccg
     1561 gaaaaacctt gtgcgtcacc ggatacagga cagcagaat
//
LOCUS       DAOQGJ010001097         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57571_length_1598_cov_3.745949, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001097 DAOQGJ010000000
VERSION     DAOQGJ010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57571_length_1598_cov_3.745949"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="PK683_15410"
     CDS             complement(<1..1591)
                     /locus_tag="PK683_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09882.1"
                     /translation="MVSLALSGLIITEGFAFTGNTFPFWTGPLLAGIGLLLVLSVKQV
                     SEFFKKPFSRVLESSHIGAFIYSGIGILYALAYTMSSDENNSALSLLSGSAQGLFGGW
                     MLYPVLGITFMLIAASLFVGSVQWKETLSTAASAGGFLFTAVSLLSLFPFGCSSALCT
                     KGTGTMLLEMFPWFSAVTAITAVAAFTASVYSEERRKDFSAGSAYSSDILIVCTGIFL
                     GIFMRISLQSSLKYSDFAGLAAISTVIFISLAAAYRNRKAIHLYFAQTAIASLYLAIK
                     PAFPDLLNDQADAIAALIFGFILTGVTAAAHRAGVPTLAESTRRFAAFMPVMASLVLP
                     AQANYQNAGMAAFSAALYAALSTVSSNRIYAVLAAASANLSIFTAVMASQVQGIEVYL
                     APFGLFSLLLGQIFKENLSDSVRKTIRLAGGLLLYLPAALKISFEIGQGADAMYAVVF
                     GLLCLIGIVAGMVFQIRSYLFMGTVFFTLNLGVNVVQVGLRDQKAGFLLLSITGILII
                     AALVFYTVKKDFVATYLSRLNK"
BASE COUNT          509 a          353 c          357 g          379 t
ORIGIN      
        1 ctttattcag acgagaaagg tacgtagcga caaaatcctt ctttacagtg taaaagacaa
       61 gtgctgcgat aatcaaaatt ccagttattg agagcagaag aaaacctgct ttctgatctc
      121 taagtccgac ttgaactaca ttcaccccta agtttagtgt aaagaaaacg gttcccataa
      181 aaagatagga tctgatttga aaaaccattc cagcgacaat tccaatgagg cacagaagtc
      241 caaagacgac cgcatacatt gcatcggcac cttgtccaat ttcaaagctg attttcaacg
      301 cggccggaag atataacagc agacctcctg caagcctgat tgtttttctc acagaatcag
      361 atagattttc cttgaaaatt tgtccaagga ggagtgaaaa aagtccaaac ggagcaagat
      421 atacttcaat tccctggact tgagacgcca tcacggcagt gaatatggaa aggtttgcag
      481 atgcagccgc aagaacagca taaatcctgt tcgatgaaac ggtggaaagt gcagcataca
      541 gtgctgctga aaatgcggcc attcctgcat tctgataatt tgcctgagca ggcagaacaa
      601 gagatgccat taccggcata aatgctgcaa acctccttgt actttcagcc agcgtaggaa
      661 ctcccgcccg atgtgctgca gcggtaactc cggtcagtat gaatccgaag ataagagcag
      721 cgattgcatc tgcctgatca ttcagcaggt caggaaacgc aggtttaatg gcaagataaa
      781 gcgatgcgat tgctgtctga gcaaaataca gatgtattgc ctttctgttt ctgtaggccg
      841 cagcaagaga aataaatatg actgtactga ttgctgccag tcctgcaaaa tcggaatact
      901 tcagagaact ctgcaatgaa attctcataa agattcctag aaatatccct gtgcatacaa
      961 ttagtatatc agatgaatat gcgctccctg cagaaaagtc ttttcgtctt tcttctgagt
     1021 aaactgaggc agtgaaggca gccacggcag taattgcagt gactgcagag aaccagggaa
     1081 acatttccag aagcattgtg ccggttcctt tcgtgcataa agcagaactg catccaaaag
     1141 gaaaaagaga cagcagggaa actgctgtaa ataaaaatcc tcctgcggaa gcggccgtgg
     1201 agagtgtttc tttccattgg accgacccga caaaaaggct tgctgcgatc agcataaaag
     1261 tgattccgag aacaggatac agcatccatc ctccgaaaag gccctgggca gatccgctca
     1321 aaagacttag tgcggaattg ttttcgtcag aagacatagt ataagccagt gcgtaaagga
     1381 tgccgattcc tgaataaata aacgctccga tatgagaact ctccaaaact ctgctgaagg
     1441 gttttttaaa aaattcagac acctgtttga ctgagagaac tagcagaagt ccgattcctg
     1501 caagaagagg gcctgtccag aaaggaaagg tgtttcctgt gaaagcaaat ccttctgtta
     1561 taatcagtcc gcttagtgcc agtgaaacca tccccata
//
LOCUS       DAOQGJ010001098         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57582_length_1598_cov_3.252106, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001098 DAOQGJ010000000
VERSION     DAOQGJ010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57582_length_1598_cov_3.252106"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PK683_15415"
     CDS             complement(<1..1074)
                     /locus_tag="PK683_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNH09883.1"
                     /translation="MDIVELEKGFHETEETIKKLAEECGNSIEIIRGGGISDTVHFIG
                     DTRKISEKEGYVKELAGVRRLWDVSIPYKNIAKVGAGKNGEVVHRPSRIVEVKGPDGL
                     VRKIGGGKHIFLVGPDSPQSKEQTLTIAKQAVEIGKKYNILDRIILRGGAFKPRTRPT
                     DWRGLGWEGIKILDEVKEETGLPYVTEVMDHTMAEEVAKHADMIQIGTRNAQDFELLE
                     AVGKTKKPVILKRGFGNESEEWFSAAEYIANQGNLDIVLCERGVKTLFIKEGYCRNTP
                     DFNVITHAKKKTILPVIFDPSHVAGDDKIVVQNLLGCLPFLADGSITETLHTEAFRKE
                     QKCDAAQALVMSIYEKAVLSILEY"
     gene            complement(1170..>1598)
                     /locus_tag="PK683_15420"
     CDS             complement(1170..>1598)
                     /locus_tag="PK683_15420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH09884.1"
                     /translation="KSPITVILIDIDFFKLYNDCYGHQGGDECLKKTAETISGIPKRP
                     GDLTARYGGEEFAVILPNTDSQGGFTVAETIRAAVISLAVPHLKSETESIVTLSLGVA
                     SVIPDKELTQENLISMADRALYEAKHKGRNRTVVFKDNKA"
BASE COUNT          351 a          381 c          324 g          542 t
ORIGIN      
        1 gtattccaga atggaaagca cagctttttc ataaatgctc attacgagag cctgtgcagc
       61 atcgcatttc tgctctttcc ggaatgcttc agtatgaagg gtttctgtga tggaaccgtc
      121 tgcaaggaat ggaaggcatc ccagaagatt ctgtacaact attttgtcgt cccctgccac
      181 atgcgacgga tcgaagatga ccgggaggat cgtctttttc tttgcatgag tgattacgtt
      241 gaagtcagga gtgtttctgc agtatccttc ctttatgaaa agagttttta cgcctctttc
      301 acatagaaca atgtccagat ttccctggtt cgcaatgtat tctgcagcag aaaaccattc
      361 ttcagattca tttccgaatc ctcttttcag tatcaccggt ttttttgttt ttccgacagc
      421 ctcgagaagt tcaaagtcct gagcatttct ggtcccgatc tggatcatat ccgcatgttt
      481 agccacttcc tctgccatag tatgatccat cacttcagtc acataaggca gacctgtttc
      541 ttccttgact tcgtcgagaa tcttgattcc ttcccatcca agacctctcc agtctgtagg
      601 acgtgttcta ggcttgaatg caccgcctct caaaatgatt ctgtccagga tattgtattt
      661 ctttccgatt tccactgcct gttttgcaat tgtgagtgtc tgttccttgg attgaggaga
      721 atcgggtcct actaggaaaa tatgctttcc gcctccgatt ttgcgtacaa gtccgtcggg
      781 gcctttgacc tcaacaatcc tgctcggtct gtggacgact tctccgtttt ttccggcacc
      841 tactttggct atgtttttgt aagggatgga tacatcccag agtcttctga ctcctgcgag
      901 ttcttttacg taaccttctt tttcggatat ttttctagta tcaccgataa aatgcactgt
      961 gtcgctgatg ccgccccctc tgatgatttc aatggagtta ccgcattctt ctgccagttt
     1021 tttgattgtt tcctcagttt catgaaaacc tttttccaat tctactatat ccaagacaag
     1081 ttacctctaa atgataattt tttactgtgc ttaaaagtgt caaatttttt cgctgtttct
     1141 ttcggcgatc tgctgaactc tcgttcagtt caagctttgt tgtccttgaa aacgactgtt
     1201 ctgttccttc ccttgtgttt tgcctcgtac aatgccctgt ctgccataga gatcagattt
     1261 tcctgggtaa gttctttatc tggaatgaca gatgcaactc ccaggctcag tgtgacaatg
     1321 ctctctgttt ctgattttaa atggggaaca gcaagtgaaa tgactgcagc tctgatagtc
     1381 tctgcaactg taaagcctcc ctgcgaatcg gtgttcggga gaatgaccgc aaattcctct
     1441 ccgccgtatc tggcagtcag atcaccggga cgtttcggga ttcctgaaat tgtttctgcc
     1501 gtttttttca gacactcgtc tccgccctga tgtccgtagc agtcattata aagtttgaaa
     1561 aaatctatat caatcagtat gactgtaatc ggggattt
//
LOCUS       DAOQGJ010001099         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57587_length_1598_cov_2.876215, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001099 DAOQGJ010000000
VERSION     DAOQGJ010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57587_length_1598_cov_2.876215"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PK683_15425"
     CDS             complement(<1..1178)
                     /locus_tag="PK683_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09885.1"
                     /translation="MQNPIEAEQNEKKTVIPVQSSEKNPIQKVRSWFLEENSLFKMPV
                     KNWHLISVLFGLLFLNLLFVLAGTMNLSGLMDSKIGIGILAVLGFVQIFIAAKLLYNA
                     GYFFFGFGILLSGAAFASDKYLFNHEVFAPFSTRTTVLLWITFSALTLSSLYRLLPGA
                     VLAVIRPLLGFGAILCIPFIITLLPICLFAWVMIPFAGLGLLPYSPIFAFILFIRMFQ
                     LSGDELAKRGQTHYQKYSFGASAVFLLMAAVYSVNFFIDWSKSYSAVRQLDSADAESQ
                     KKQDIFPKWARAAEKLEKSDALEYFLMPERRVRNSSMFSSGKKYDPLSFLSSLIFSNT
                     ESGLSEEEKNRLLRFFFGYSDEELERLWSGVSLVTESQESVVEIDLASRTAYTETKLK
                     "
     gene            complement(1251..1454)
                     /locus_tag="PK683_15430"
     CDS             complement(1251..1454)
                     /locus_tag="PK683_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09886.1"
                     /translation="MIGTPDTTIETTTVMQEYLAEIYRMTGNLKRAESVLSEILMISP
                     EEEKAKDLLSRLDLAFNESEYSF"
BASE COUNT          492 a          346 c          331 g          429 t
ORIGIN      
        1 atcttcagct ttgtttcagt ataggcagtt ctggatgcaa gatcaatttc cacaaccgat
       61 tcctgggatt cagtgacaag agatactccg ctccagagtc tttccagttc ttcatcggaa
      121 tatccgaaaa agaacctgag aagcctgttt ttttcttcct cagaaagacc gctctcggta
      181 ttcgaaaaaa tcagtgagga taaaaatgat aaaggatcat actttttacc ggaagaaaac
      241 atgcttgaat ttctgactct tctttcgggc atcaggaagt attcaagcgc atccgatttt
      301 tcaagtttct ctgcagctct ggcccatttc ggaaaaatat cctgtttttt ctgagactct
      361 gcatcggcag aatccagctg tctgactgca ctgtaagact tgctccagtc tatgaaaaaa
      421 ttcaccgaat aaactgcagc cataagcagg aacacggcag aagcaccgaa cgaatacttc
      481 tgataatgtg tctgacctct tttcgcaagc tcatcgcctg aaagctgaaa catccttatg
      541 aaaagaataa atgcaaatat cggactgtaa ggaagaagtc caagacctgc aaaaggaatc
      601 attacccagg caaaaaggca gatcggcaga agagtgataa taaacggaat gcaaaggatt
      661 gcgccgaatc cgagaagagg acggatgact gcaagaactg caccgggaag aagtctgtac
      721 aatgaggaaa gagtcagggc actgaatgtg atccagagaa gcactgtggt ccttgtactg
      781 aaaggtgcaa atacttcatg attgaagaga tatttgtcgg aagcgaatgc tgcacctgaa
      841 aggaggattc cgaatccgaa aaagaaataa ccggcattgt acaaaagttt agctgcaatg
      901 aatatctgaa caaagcccaa tacggccaga attcctattc caattttgga gtccatcaga
      961 cctgacagat tcattgttcc tgccaaaaca aaaaggagat tcaaaaaaag aagcccgaac
     1021 agaacagaaa tcagatgcca gttctttaca ggcatcttaa acagactgtt ttcttctaaa
     1081 aaccagcttc ttactttctg aatcggattt ttttccgaac tctgtaccgg gatgactgtc
     1141 tttttttcgt tctgctctgc ttcaattggg ttctgcattt atgactcttc ctgaaacaga
     1201 atataaattt ccgatcctct tctgtaaaga aaaaagcaga tcagcaaatc tcaaaaactg
     1261 tactcagatt cattgaaagc cagatccagg cgcgagagca gatcttttgc tttttcctct
     1321 tcaggactga tcatcaggat ttcactcaga acagactctg cccttttcag gttgcctgtc
     1381 atacggtaaa tttctgccag atactcttgc atgaccgttg tcgtttcgat ggtcgtgtca
     1441 ggtgttccga tcattgagcc gcctttagct gttgtcgttt gagagtataa taaaaaagat
     1501 caacgttttc aaaaatattg tactggattg acggggcatg tcaagcgggc tcacaaggga
     1561 tagccccaaa atagtgccac ttcgttatct tccacatc
//
LOCUS       DAOQGJ010001100         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57664_length_1597_cov_2.099870, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001100 DAOQGJ010000000
VERSION     DAOQGJ010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57664_length_1597_cov_2.099870"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="galT"
                     /locus_tag="PK683_15435"
     CDS             complement(<1..771)
                     /gene="galT"
                     /locus_tag="PK683_15435"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNH09887.1"
                     /translation="MSEIRRNMITRDWVIMATERARRPHEFIDGKKQRAVLPHYKDNC
                     PFCIGNEHLSTKEYARIDGPDGWKVRIIANKFPALSPEGNRVRHTEGLYQTISGIGVH
                     DVVVESPEHDAVMAFMPETQIQNILRCYRERYLQIRKDRRLEAIVIFKNYGESAGSSL
                     EHTHSQIAATPVVPPQFRDRVEEAVRYFDDHGDCIFCRTILDEISTGSRIIAENSNFI
                     AFIPYAALTPFHIWVFPRIHNSSFESISDDEIADLASIM"
     gene            complement(867..>1597)
                     /locus_tag="PK683_15440"
     CDS             complement(867..>1597)
                     /locus_tag="PK683_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH09888.1"
                     /translation="SCAESLLNLDREMKSSIVLAGESSGLSEKTHQYAQDGAGLGLSA
                     EAAITEILEESRAIIDYAHLISEIADQVGLLSLNASIESARAGESGRGFKVVAGEISK
                     LGESTNANSEMISKKVAVLSKRIKFGYEKILDVSKKFSEIQEASSRTSQSMELISENL
                     SRQAKMQGQVKQLILELKDSAGSIRDSAKEQRLIIEESNSGLERLTESSELLSVSAES
                     LRDISIEMKKDAIQLFSQIEFFRI"
BASE COUNT          406 a          394 c          311 g          486 t
ORIGIN      
        1 cataatggaa gccagatctg cgatctcatc gtcggaaatg ctttcaaagg atgaattatg
       61 gattctaggg aatacccaga tatgaaaagg tgtgagtgcc gcataaggga tgaatgcgat
      121 aaagttggaa ttctctgcaa tgattcttga tccggtagaa atttcatcta aaattgttct
      181 gcagaaaatg cagtccccat gatcatcgaa gtatctgact gcttcctcaa ctctgtccct
      241 gaactgagga ggcacaacag gcgttgcagc aatctgtgag tgagtgtgtt caagtgacga
      301 acctgcagac tctccgtaat tcttgaatat tacgatcgct tcaagcctcc tgtccttcct
      361 gatctgaaga tatctttctc tgtagcatct gagaatattc tgaatctgcg tctcaggcat
      421 aaacgccata acagcatcat gttcaggact ttccactacc acatcgtgca caccgattcc
      481 ggaaattgtc tggtacagtc cttctgtatg tctcacccgg tttccttcag gagacagtgc
      541 aggaaattta tttgcaatga ttctgacctt ccatccgtcc ggtccgtcaa tccttgcata
      601 ttctttcgtt gacagatgtt cgttgccgat gcagaaagga cagttgtcct tgtaatgagg
      661 aagaaccgcc ctctgttttt ttccgtcaat aaattcatga ggtcttctgg ccctttctgt
      721 cgccataatg acccagtccc ttgtaatcat atttcttctg atttcggaca tatccccccc
      781 tgacctgcac cacagatccc ggatctcagt atactgatct gtccgtaaag gtaaagaaaa
      841 atactatctc cgaagactgg aataaatcat atcctgaaaa actctatctg actgaaaagc
      901 tggatcgcgt cttttttcat ttctattgaa atatcccgaa gactttcggc agatacagaa
      961 agaagttcag agctttcagt cagtctttcc agacctgaat tggattcctc tatgatcagt
     1021 ctctgctcct tggcagaatc tcgaatggag ccggcactgt ctttcagttc cagaatcagc
     1081 tgtttcacct gaccctgcat tttcgcctgt ctgctcagat tttctgaaat cagttccata
     1141 gactggctgg ttctggaaga tgcttcctgt atttcgctga attttttaga tacgtccaga
     1201 atcttctcat atccgaattt gattctcttt gaaagcacgg ccactttctt ggaaatcatt
     1261 tccgagttcg catttgtgct ttctccaagc ttggaaattt ctcctgcaac aactttaaag
     1321 ccccttcccg attcccctgc tcttgcagat tcaatggaag cattcaggga aagcagtccc
     1381 acctgatcgg caatttcaga aatcagatgt gcataatcta taattgctct ggactcttcc
     1441 agaatttccg tgattgcagc ttccgcactt aatcccagac ctgctccatc ctgtgcatac
     1501 tgatgtgtct tttccgaaag acctgaagat tcaccggcca atacaatgct ggacttcatt
     1561 tccctgtcaa gattcaaaag tgattcagca caggact
//
LOCUS       DAOQGJ010001101         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57714_length_1596_cov_2.537962, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001101 DAOQGJ010000000
VERSION     DAOQGJ010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57714_length_1596_cov_2.537962"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PK683_15445"
     CDS             complement(<1..818)
                     /locus_tag="PK683_15445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNH09889.1"
                     /translation="MIEGKPSETAQIVCLMRALEYRWNSDKSLLSDKYAEFFLTGRFK
                     FWHSFLSGVSGRILFPAFQWLYDAAVLRSALMDEYIRKHGNECPVVILGAGFDSRSVR
                     MKDQMRQGVYEIDFPATQDYKRSVLRENGIDDSHVFYYNADFMKNTLLEILSGLDLHG
                     KRVILIWEGVSMYLTEDLVYETVRNIGQYFGKDSVLIADFWLQSFALPEFINRSVQNL
                     FQILWDEPILFGTSPEELKKNLEERDGFECTIMSYDSLSEMFSVQKTLYSPFFTA"
     gene            complement(973..1389)
                     /locus_tag="PK683_15450"
     CDS             complement(973..1389)
                     /locus_tag="PK683_15450"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNH09890.1"
                     /translation="MISDSVHFKIIIMFDGVCNLCNAAVNFLIDHDSQDVFRFVSLQS
                     DTGKSLLEKSPLNIQNIDSVVVIDKNEYYIKSTAVFKIAHFLPFPFSIIKMGRVLPVS
                     FSNRIYDLIASSRYSLFGKKDVCRMPTPEIRAKFLD"
     gene            complement(1386..>1596)
                     /locus_tag="PK683_15455"
     CDS             complement(1386..>1596)
                     /locus_tag="PK683_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09891.1"
                     /translation="IRKNADLLKREEIKLKGGKGSLIVFLLREDLYYESKLSLLELQM
                     QKARYVSEIFPLSEVLIRKNSEERK"
BASE COUNT          499 a          351 c          283 g          463 t
ORIGIN      
        1 gcagtaaaaa aaggagaata cagtgttttc tgaacagaaa acatctccga gagagaatca
       61 tagctcataa ttgtgcattc aaacccgtct ctctcctcaa ggtttttttt cagctcctcg
      121 ggtgaggtgc cgaaaagaat cggttcatcc caaagaatct gaaaaagatt ctggacggat
      181 ctgttaatga attcaggaag tgcaaaagac tgaagccaga aatctgcaat aagaaccgag
      241 tcttttccga aatactgtcc gatatttctc accgtttcgt aaacaaggtc ctcagtgaga
      301 tacatggaaa caccttccca tataaggatg acccttttcc cgtgcaggtc cagtcctgaa
      361 agaatttcca gaagagtatt tttcatgaaa tctgcattat aataaaacac atgtgaatca
      421 tcaatgccgt tttctctcag aactgaccgt ttataatcct gtgttgcggg aaagtcaatt
      481 tcataaaccc cctgccgcat ctgatccttc attcggacgg atctggaatc aaatcctgct
      541 ccaaggatga caacagggca ctcatttcca tgttttctga tgtattcatc catcagtgct
      601 gaccttagaa cggctgcatc ataaagccat tgaaatgcag gaaaaagtat cctccctgaa
      661 actcccgaaa gaaacgaatg ccagaacttg aaacggccag tcaggaaaaa ctcggcgtat
      721 ttatctgaaa ggagtgattt gtcactgttc catctgtatt ccagtgccct catcaggcag
      781 acgatttgag ctgtttcact cggttttcct tcaatcatat attcctcctg tattcagaat
      841 agtattctct ttccgttctt ttaaagcctg atcataaagt tcagagatat gcagatcggg
      901 aattccttca aaactccaga gctgccgaca attagaccgc tgaatctgtt tcttttcttc
      961 agcgaacttt tgttaatcaa gaaactttgc acgaatttcc ggcgtcggca ttctgcagac
     1021 atcttttttt ccgaaaagac tgtacctgga tgaggctatc agatcataaa tccggtttga
     1081 aaaagaaacg ggcagaactc tgcccatctt tataatcgaa aaaggaaaag gaagaaaatg
     1141 agctattttg aaaacagcag tggacttaat ataatattca tttttatcaa ttacgacaac
     1201 cgaatcaatg ttctgaatat tcaaaggtga cttttccaga agactttttc ctgtgtctga
     1261 ctgaagagaa acaaatctga acacatcctg actgtcatga tctatcagga aattcacagc
     1321 cgcattgcat aaattgcaca ccccgtcaaa cataataatg attttaaaat gaacagaatc
     1381 agaaatcatt ttctctcttc cgaatttttc ctgatcagaa cttcagagag agggaaaatt
     1441 tcagaaacat atcttgcctt ctgcatctgc agttcaagaa gagaaagctt gctttcataa
     1501 taaagatctt ctctcagcag aaatacaatc agagaacctt ttccaccttt cagtttaatc
     1561 tcttctctct tcaaaagatc tgcatttttc ctgatc
//
LOCUS       DAOQGJ010001102         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57935_length_1592_cov_3.518543, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001102 DAOQGJ010000000
VERSION     DAOQGJ010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57935_length_1592_cov_3.518543"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..>1592
                     /locus_tag="PK683_15460"
     CDS             178..>1592
                     /locus_tag="PK683_15460"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH09892.1"
                     /translation="MKKLPIGIQTFSEIRTGNYYYVDKTKVLSELTESGKYYFLSRPR
                     RFGKSLFLDTLREAFLGSKEYFKGLYLEHNWDWTTKHPVIRIDFGTGHLRTASELDQR
                     ILEILNEHAAKNSVSFKNESISGKFRELIMALQEKNSEKVVVLIDEYDKPILDNITDK
                     PLAREMRDGLRNLYSVIKGADAYIKFAFITGVSKFSPLSSPLLGGTVGGSGLNNLQDI
                     TLDERYATVCGYTDNELSVFLERLEDVNRDKLKLWYNGYSFLGDRVYNPFDILLFLDQ
                     KKYKSYWFETGNPSFLIDLIREKNYNSINMNKVRLTDSALSSFDVDSIELENLLFQTG
                     YLTIKSIKEMQANRVYYLQYPNLEVRSSLTDVILTYLAKDTQPKEINKVDLYDALQEN
                     DLSKLKNIFYSFFASIPYDWYRKNTIAQYEGYYASIFYCYFTALGLDVRAEDVTNQGQ
                     ADLTVFFEDRVYVFEFKVIEMT"
BASE COUNT          491 a          319 c          342 g          440 t
ORIGIN      
        1 gatgaaccct gatgatccaa aaacactgaa actgacccgg aaaatcaagg aaaagataga
       61 gcagagaagg cttcaggaac agcaggtttg aggattgcag gcacgcacgg ccctgcgtcc
      121 ctacggcaga agcatcaact gcttgacaga gagctgcctt ttattcaact tagttctgtg
      181 aagaaacttc cgatcggcat tcagactttt tcagaaatca gaactggcaa ttattattat
      241 gtggataaga ccaaggttct gagtgagctc acggaatccg ggaaatatta ttttctctca
      301 cggccccgcc gtttcgggaa gagtttgttt ctggatacac tgcgggaggc ttttctcggc
      361 agtaaagaat acttcaaagg tctttatctg gaacacaact gggactggac aacgaaacat
      421 cctgtcattc ggatagattt tggaacagga catcttcgga cagcttcgga actggatcag
      481 aggattctcg aaattctgaa tgaacacgcg gcgaaaaatt ccgtctcatt caaaaatgaa
      541 tccatttcag gaaaattcag agaactgatt atggcacttc aggaaaaaaa ttcagagaaa
      601 gttgtcgttc tgatcgacga atacgacaaa cccattctgg acaatattac cgacaaacca
      661 ctggccagag aaatgagaga cggcctgaga aatctgtatt ctgtaatcaa aggtgcggat
      721 gcatacatta aatttgcatt tatcacaggc gtatctaaat tttcccccct ttcatccccc
      781 ctattggggg gaacagtggg gggcagcggt ttgaacaatc ttcaggatat cacattggat
      841 gaacgttacg caactgtctg cggatataca gataatgagc tttccgtttt tctggaaaga
      901 cttgaagacg tcaacagaga taaattaaaa ttatggtata atggttacag ttttctgggc
      961 gaccgtgtat acaatccgtt tgatatttta ctttttttag atcagaaaaa atataaaagc
     1021 tactggttcg aaactggaaa tccttccttt ctgattgatc tgatccgcga aaaaaattat
     1081 aattccatca atatgaacaa ggtcagactg actgattccg ccctttcgag ttttgatgtg
     1141 gattctatcg aactggagaa tcttctgttt cagaccggat acctcacaat aaaaagcatc
     1201 aaggaaatgc aggcaaatcg agtctattat cttcagtatc ccaatctgga agtcagaagc
     1261 agtctgacag atgtaatact gacctatctg gctaaggaca ctcaacccaa agaaatcaac
     1321 aaggtggatc tctatgatgc ccttcaggaa aatgatcttt ctaaactgaa aaatattttc
     1381 tattccttct ttgcctccat tccctatgat tggtacagaa agaatacaat tgctcagtat
     1441 gaaggatact atgcatctat attctattgt tactttactg cccttggtct ggatgtgcgt
     1501 gcagaggacg taacgaatca gggacaggca gatctgacag ttttttttga agaccgagtt
     1561 tatgttttcg agttcaaggt gattgagatg ac
//
LOCUS       DAOQGJ010001103         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_57987_length_1591_cov_4.086589, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001103 DAOQGJ010000000
VERSION     DAOQGJ010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57987_length_1591_cov_4.086589"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /gene="htpG"
                     /locus_tag="PK683_15465"
     CDS             complement(<1..1340)
                     /gene="htpG"
                     /locus_tag="PK683_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNH09893.1"
                     /translation="MSTETGKVSVQTENIFPIIKKWLYSEKDIFLRELVSNAVDAITK
                     LKKISLSEEFEGGTDYKVHISFNQEARTLTIQDNGVGMSADEVKKYINQIAFSGAEDF
                     VKKYQSAEKESEGIIGHFGLGFYSSFMVASRVIIETKSYRKGEMAVRWESESGVEYTI
                     TPIDDRDERGTKIILVMDSESGEYLDKWKLKDLVKKYCDFMPVPIYVEEEQANRQKAL
                     WSENPASLKKEDYTQFYNYLFPFAGEPHFWIHLNVDYPFRLQGILYFPKVTHELDISK
                     SAIKLYCSHVFVSEEANELIPQFLTVLKGTIDIPDLPLNVSRSYLQTDPLVKKISAHI
                     VKKLADKLGEEFKKDKAEFEKIWEDIALFVKFGMMNDEKFYEGVKNIVLFKSSEGGYV
                     TLDDYWTRNKEKNGGKVFYAPDIESGSVYMDLFKEQGLEALFADSKIDTHFLQFL"
     gene            complement(1342..>1591)
                     /locus_tag="PK683_15470"
     CDS             complement(1342..>1591)
                     /locus_tag="PK683_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09894.1"
                     /translation="FRNTAVFPGIPDDIHSFDILAGSKKEVLLKNFLCTKGYSPANAE
                     EIVHFRKKNQNLSFAELYKSLDRHGRKRLLELVRSKES"
BASE COUNT          422 a          380 c          299 g          490 t
ORIGIN      
        1 aggaactgca ggaaatgagt gtctattttg gaatcggcaa ataatgcctc aagcccctgc
       61 tctttgaaaa gatccatata taccgaaccg gattcaatat cgggagcata aaaaaccttg
      121 cccccgttct tttccttgtt ccttgtccag taatcgtcaa gagtcacata tccgccctct
      181 gaggacttga acagaactat attcttcacg ccctcgtaaa acttttcatc attcatcatc
      241 ccgaatttta caaaaagagc aatgtcttcc catatctttt caaattctgc cttgtccttc
      301 ttgaactcct caccgagctt gtctgcaagt tttttgacta tatgtgcaga tattttcttc
      361 acaagaggat ctgtctggag gtaggaacgg gaaacgttca gaggaagatc cggaatgtcg
      421 attgtccctt tcagaaccgt gagaaactga ggaatcagtt catttgcctc ttcgctgaca
      481 aatacatgac tgcagtagag tttgattgca gacttgctga tgtccagctc atgagtgact
      541 tttggaaaat acagtatccc ctgaagcctg aaagggtagt ccacgttcag atgaatccag
      601 aaatgcggct cgcctgcaaa agggaaaaga taattataaa attgagtata atcctctttt
      661 tttaatgaag caggattttc cgaccagagt gctttctgtc tgtttgcctg ctcctcttcc
      721 acatagatag ggaccggcat aaaatcgcag tatttcttga ccagatcttt cagcttccat
      781 ttgtccagat attctccgga ttcggagtcc atcacaagaa tgatcttcgt gcccctttca
      841 tcccgatcat cgataggagt gatcgtatac tcaacaccgg attcgctttc ccagcggaca
      901 gccatttcac cctttctgta agacttagtc tctataatga ctcgggacgc caccatgaag
      961 gaagaataaa aacccagtcc aaaatgaccg atgattccct cagactcctt ttctgcagac
     1021 tgatacttct tcacaaagtc ctcagcaccg ctgaaagcaa tctgattgat gtattttttt
     1081 acctcatctg cgctcattcc gaccccgttg tcctggatgg tcagtgtgcg tgcttcctga
     1141 ttgaaactga tatgcacctt ataatcagtt ccgccttcaa actcttcgga taaagatatc
     1201 tttttaagtt tagtgatcgc atccactgca ttggacacca gctcacgaag gaatatatct
     1261 ttttccgaat acagccattt ttttataatt ggaaaaatat tctcagtctg aaccgagacc
     1321 ttgccggttt ctgtgctcat tttaactctc ctttgatctt acaagttcca acagtctttt
     1381 ccgtccgtgt ctgtcaagcg atttataaag ttcagcaaaa ctcaaattct gatttttctt
     1441 tctgaaatgg acaatttctt cggcgtttgc gggactgtac ccctttgtgc agagaaagtt
     1501 cttcagaaga acttctttct tgcttcctgc cagaatatca aatgaatgaa tgtcatccgg
     1561 gattccggga aaaactgcag tgttcctgaa t
//
LOCUS       DAOQGJ010001104         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58059_length_1590_cov_3.646906, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001104 DAOQGJ010000000
VERSION     DAOQGJ010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58059_length_1590_cov_3.646906"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1388
                     /gene="rmuC"
                     /locus_tag="PK683_15475"
     CDS             90..1388
                     /gene="rmuC"
                     /locus_tag="PK683_15475"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNH09895.1"
                     /translation="MDIFFILIIIVGLIQTAVSIALFYQLLKKDDDKNAESIREIKNR
                     LGTLESQIREEFRISRRENNDTGQAIRQESFSVLTKMNDSINKTLESNRHDIQAGIKS
                     IESKFELIRRTMESRIESMQSENSKKLDEMRHVVEEKLQSTLEKRLSESFKSVSERLE
                     QVHQGLGEMQSLASGVGDLKKVLSNVKTRGVLGEIQLANILENILAPDHYEKNVITVP
                     GTRENVEFALKLPGRKESDSNVYLPIDSKFPYELYHYLVDAHEKGNPDEVKTALKNLS
                     TEIKKQAKTISEKYLSPPHTTDFGIMFLPFEGLYAEVARQPELLDHLQKEYKIIITGP
                     TTLAALLNSLQLGFKTLAIEKRSSEVWNVLSAVKTEFGKFSVVLKKARENIANAGKEI
                     DRLVTTRTDQINRKLKTLESLPSEESEKFLLFDEVEKPED"
BASE COUNT          533 a          306 c          348 g          403 t
ORIGIN      
        1 gaattcaaca atcgactgca cattgtctgg gtggaaacaa acggaagttt tgatcaggtt
       61 cgggtggcgc agttcaacgg gaactgatat tggatatatt tttcatactt atcattatag
      121 taggactgat tcagaccgca gtctcaattg cactgtttta tcagctcctg aaaaaggatg
      181 acgataaaaa tgcggaatcc attcgggaga ttaaaaatcg tctgggaact ctggaatctc
      241 agatccgcga ggaattccgc atcagcagaa gagaaaacaa tgatactgga caggcaatac
      301 gccaggaatc tttttctgtt cttacaaaaa tgaatgattc catcaacaag actctggaat
      361 cgaacaggca tgatattcag gctggaatta aatccattga atccaagttt gaactgatcc
      421 gaagaacaat ggaatcccgg attgagtcaa tgcagagcga aaactccaag aagctggatg
      481 agatgaggca tgttgtggaa gaaaaacttc agtctactct ggagaaaaga ctttctgaat
      541 cattcaagtc agtcagtgaa agactggaac aggtgcatca gggtctgggt gaaatgcagt
      601 ctctcgcttc aggtgtaggc gatctgaaaa aagtcctctc caatgtgaag acgagaggcg
      661 tgcttgggga aatccagctt gccaatattc tggaaaatat tcttgcaccg gatcattatg
      721 aaaagaatgt aatcacagtt cctggaacaa gggaaaatgt ggaattcgct ttaaagctcc
      781 ctggacggaa agaatcggac agcaatgttt atctacccat tgattctaaa tttccttatg
      841 aactgtatca ctatcttgta gatgctcacg agaaaggaaa tccggatgag gttaagacag
      901 ctttgaaaaa cctctccact gaaatcaaaa aacaggcaaa aacgatcagt gaaaaatatc
      961 tttccccgcc ccatacaact gatttcggaa ttatgttcct tccttttgaa ggactgtatg
     1021 cagaagtggc aaggcagccg gaactgctcg atcatcttca gaaagaatac aaaatcatta
     1081 ttacaggacc gactactctt gctgctcttt taaacagtct tcagctggga ttcaagacat
     1141 tggcaatcga aaaaagatcc agtgaggtat ggaatgtgct cagtgcagtc aaaactgaat
     1201 tcggaaaatt ctccgttgtt ctgaaaaaag caagagagaa tatagcaaat gcaggaaagg
     1261 aaatagacag gctggtcaca acccgtactg atcagatcaa tcgaaagctg aagactctgg
     1321 aaagccttcc ttcggaagaa tcggaaaaat ttcttttatt tgacgaagtc gagaaaccgg
     1381 aggactgaat ttcctgtata ttaaacctgc ggacattttg ctgaaaaatt ataaaacttt
     1441 ttagtcaatc aggcagttct aaagattggg atacatctgt ttacaaaaac actatgtgag
     1501 gctgtgagag ctctcataaa gcaaagaatg attataaacg ccaatcataa gacaatctga
     1561 aacccatccc ctgctggact gacattctgt
//
LOCUS       DAOQGJ010001105         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58142_length_1589_cov_3.493481, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001105 DAOQGJ010000000
VERSION     DAOQGJ010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58142_length_1589_cov_3.493481"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PK683_15480"
     CDS             complement(<1..915)
                     /locus_tag="PK683_15480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNH09896.1"
                     /translation="MRACIIRKYGNNDVLEYTNLQPEPTLHDHEILVQMKAASVNPID
                     WKVMEGKAKMVLKYKFPLVLGNDGAGTVVKTGKDVTQFKIGDEVFFRPSKTRIGTFAE
                     FCAVSENDAALKPKNLNFEEAAGIPLAGLTSWQALFDYGRLEKGQKVLIHAGSGGVGS
                     LAVQFAKNAGAFTIATCSTRNIDLVKSLGADQVIDYTKEKFTDILKDVDIVFDTIGGQ
                     AKYDSFKVMKPGGTLISIQSVPTPKSVKDYGLNPVVEMLLGILSLKSYRYARKYKVNY
                     HYLFMKADGKQLNEIKNLIEEGKVKPVVD"
     gene            complement(925..>1589)
                     /locus_tag="PK683_15485"
     CDS             complement(925..>1589)
                     /locus_tag="PK683_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09897.1"
                     /translation="SCTGGGAVFDGISASGCLGIGQSDFTNQATDYSSVNPFISNSPK
                     SSSDTVNSDFSTGTKTYASNLSWGGFQNFYRTAGKENANPFPSTNLLSTCSGASTCRL
                     YDWSFSSSDSTARNSVVCPEKLSRPTLTVSSQTILRNTYEILGDSIGDDDGLCESNEE
                     CIYTPNIGAYQGHGNLVPASTANTYYASSQNQCSDAISTVSGSDGTVTNIKLYKYEKN
                     GY"
BASE COUNT          396 a          369 c          310 g          514 t
ORIGIN      
        1 gtccactacc ggttttacct tcccttcttc tataagattt ttaatctcat tcagctgttt
       61 tccgtccgct ttcatgaaaa gataatgata gtttactttg tatttccgcg catatctgta
      121 tgacttgagt gaaagtattc ctaaaagcat ttctacaacc ggattcagtc catagtcttt
      181 aactgatttc ggagtaggta cgctctgaat tgaaatgaga gttcctcccg gtttcattac
      241 tttgaaagaa tcatatttcg cctgtccgcc tatcgtatca aaaacgatat ccacatcttt
      301 cagtatgtcc gtaaattttt ccttagtata atcaataacc tggtcggcac cgagactctt
      361 cactaaatct atatttcttg tgctgcaggt cgctattgta aaagcaccgg cattttttgc
      421 aaactggact gccagacttc caacaccgcc tgaaccggca tgaatcagaa ccttctgtcc
      481 tttttccagc ctgccatagt caaataacgc ctgccaggaa gtgaggcctg ccagaggaat
      541 tccggcagct tcttcaaaat tcaggttctt aggttttaat gcagcatcat tttcactcac
      601 tgcacagaat tcagcaaagg tccctatcct ggttttgctg ggtctgaaaa atacctcgtc
      661 tccaatttta aattgagtga catctttccc tgtcttgact acggttcctg ctccgtcatt
      721 tccgaggact aaagggaatt tgtatttcag aaccattttt gccttccctt ccataacctt
      781 ccagtcgatt gggtttacac tggcggcttt catctgcact aaaatttcat gatcgtgaag
      841 cgtcggttcc ggctgaagat ttgtatattc cagcacatcg ttgtttccgt attttctaat
      901 gatgcaggct ctcatcttgt ctcctcagta cccgtttttc tcgtatttat acagtttaat
      961 attcgtcacc gtcccatcag aacctgagac cgtggatatc gcatctgagc actgattctg
     1021 tgaggatgca taatatgtgt tcgcggtgga tgcgggaact aaatttccgt gtccctgata
     1081 agctcctata ttcggagtat aaatacattc ctcgttggat tcgcacagcc cgtcatcatc
     1141 tccgatcgaa tctcccagta tttcatatgt atttctcaaa attgtctgac tggaaacagt
     1201 cagtgtaggt ctgctcagtt tttccggaca gacaactgaa tttcttgctg ttgagtcaga
     1261 ggacgaaaaa gaccagtcat acagtctgca agtagatgcc ccgctgcatg tactgaggag
     1321 atttgtgctt ggaaaagggt ttgcattctc ttttcctgca gttctgtaaa aattctgaaa
     1381 tccgccccag gagagatttg atgcatatgt ttttgttccg gttgaaaaat cagaattgac
     1441 tgtgtctgat gatgatttag gtgaatttga tatgaatggg tttacggaag aataatctgt
     1501 tgcctgattt gtgaaatccg actgcccaat accaagacat ccgctcgcgg aaattccgtc
     1561 aaaaacagct cctcctcctg tacaggaac
//
LOCUS       DAOQGJ010001106         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58149_length_1589_cov_3.353977, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001106 DAOQGJ010000000
VERSION     DAOQGJ010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58149_length_1589_cov_3.353977"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1134
                     /locus_tag="PK683_15490"
     CDS             64..1134
                     /locus_tag="PK683_15490"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH09898.1"
                     /translation="MCLILALIVSCKEKNEQKPAENAPKADSSGLVLFVIGDVTNGGA
                     KVKLGDTIKPKDLLKVGKKSICDMQIKGTEITMRVKELSEFSFESKTVGSKEDISAEL
                     QSGSAIFNVQKMKSENGFSVKTPAHVVGVRGTKFETSVTADGKSKTNVYEGKVAAKIR
                     IKELENISEEELRKSKNLTALLDSVSNTEVVLEKGQSTVGDIKQAEKILKDTGLAEAV
                     KNLDSAKVADIDTKIDPAKTLEKTAKIAKEAKPSVAAIPPADFEKKLKEYEEIIAVES
                     SKLNDPNLSKTALTERAKQNEERILKRIEEITGKALEILVLKNGTKIKGVIYQEGDKY
                     IILTSSGKEEYEAKDVEGTEFN"
BASE COUNT          585 a          263 c          338 g          403 t
ORIGIN      
        1 tactctaata tttttaatca tttttcagtt ttgcgtggct gtcgtttcgg acaactgtac
       61 agtgtgtgtt taattcttgc gctgatcgta tcctgtaagg aaaaaaacga acaaaagcct
      121 gctgagaatg ctccaaaagc agattcttcc ggtttggttc tgtttgtaat tggagatgtt
      181 acgaatggcg gcgctaaggt caaactgggt gatacaataa aaccaaagga tcttctcaaa
      241 gttggaaaaa aatcgatttg tgatatgcag atcaagggta cagaaattac aatgagagtc
      301 aaagaacttt cggaattcag ctttgagtca aagactgtag ggagtaaaga agacatttcc
      361 gctgaactgc agtcgggatc agctattttc aatgtccaga aaatgaaatc agagaatggc
      421 ttcagtgtaa aaactcctgc tcatgtagtc ggtgttcgag gaacaaaatt tgaaacatca
      481 gttactgcag atggaaaatc aaagacaaat gtttatgaag gaaaagtagc tgcaaaaatc
      541 aggatcaaag aacttgaaaa catttcggaa gaagaactta gaaaaagtaa aaatttaact
      601 gctcttctgg attcagtctc aaacactgaa gtcgtattgg aaaaaggaca gagtacggtc
      661 ggagacataa aacaggcaga aaagattctg aaggatactg gtcttgctga agcagtgaaa
      721 aacctcgatt cggctaaagt tgcagatatt gatacaaaga tagacccagc aaaaactctg
      781 gaaaaaactg cgaaaattgc gaaagaagca aaaccttctg tggcagcaat tccacctgct
      841 gattttgaaa agaagctaaa agaatatgaa gaaattattg ccgtagaatc atcaaaactc
      901 aatgatccta acctgtctaa aacggcgttg acagaaagag caaaacagaa tgaggaaaga
      961 atactgaaaa gaatcgaaga gataactgga aaagccctgg aaattctggt actcaaaaat
     1021 ggaaccaaaa tcaaaggagt catttatcag gaaggtgata aatacattat cctgacttca
     1081 tctggaaaag aagaatatga agcgaaggat gtagaaggaa cagagtttaa ctgatttcgg
     1141 cttacacttg cattcaacgt gcttacaaaa aaacgagggt atctcgcagt aatttctgca
     1201 caacttcagg ataccttaat acccggattt ccagtagacc aaagggcgga acagggctgc
     1261 ggcttccacg cctgcgaaat tagaaatctg ggtaatagat ttatgcactg acttcaggct
     1321 ggtcataaat caggaactgt aaatttttct cataaagact aaacaatact tcacataaaa
     1381 aaagaagggt tatagctgtt actgtatttt taaatataat aaaacttctt caatgagatt
     1441 tataaaaaaa ctgccttcaa actgattttc agaatataaa gagaataaaa aataaggaag
     1501 aatgtttttg caggactgat tacctgcgtt gcaagtgaag cttttgcaga ccggcggcag
     1561 gatcttcaat ttcggtcgca agcttgaca
//
LOCUS       DAOQGJ010001107         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58173_length_1589_cov_2.158409, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001107 DAOQGJ010000000
VERSION     DAOQGJ010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58173_length_1589_cov_2.158409"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..670)
                     /locus_tag="PK683_15495"
     CDS             complement(176..670)
                     /locus_tag="PK683_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09899.1"
                     /translation="MKLIKFIIITLFIIECTSPPKKETEDAPKKEENSQPTDVRFFEL
                     KKDGTEVYTVKAEHIEASVSKKGQITLGLWKNGKAYDLLFGHLNDAYAEGMGTTVISV
                     KVGEKVKDFLDLQNLKVVKMDKNVIEVQGKWPDREIYVTEKIERVPDRDSIRLFVYIK
                     NNEK"
     gene            complement(667..>1589)
                     /locus_tag="PK683_15500"
     CDS             complement(667..>1589)
                     /locus_tag="PK683_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09900.1"
                     /translation="VTVPAKTEPALPEKVITAYPAVREKKNPYITFDGQIFTDGKVPF
                     SLKSTDDVSAGFTEFSLNGKEFQTYKEPIPVTEEGKNTLVYRSRDMLGNLEMAKKKEI
                     FVDKTRPKIILAPHSRYAENMGKKYFSPGAEFRLEASDKDSGVREIRYSVDSQSWEVF
                     YADYLRIYKDGEHSVTAVSEDNVGNVSEQATVFFNVDSTPPEVHLSYFPVMKFKGKDY
                     CKLPVTFHVDAADYGAGVDNIQYKFQNTEWSLTDGDIRITETEQDELMIAVRARDKVG
                     NTSSVKAFRCLIDRTPPKTEFGAETAGEGK"
BASE COUNT          388 a          351 c          296 g          554 t
ORIGIN      
        1 cttccattgc tgaaaaaatc tcaatttctt ttatcatatc atttatgctt aaaatttctt
       61 tagaaaattc attagttttt ctttcaaaaa aatacagaaa tttttctttt ttatagatgt
      121 tacttattaa agttactgaa aatcataatt ttgttggata tatagaaagt gtgtgtcatt
      181 tttcattatt ttttatataa acgaagagtc tgatagagtc tctgtccgga actctttcaa
      241 ttttttcggt cacataaatt tccctgtcag gccattttcc ctgaacctca atcacgtttt
      301 tgtccatttt tacgactttc aaattctgaa gatcgagaaa gtccttcacc ttttctccga
      361 cttttacgga aatcactgtg gttcccatac cctcagcgta agcatcgttc agatgaccga
      421 aaagcagatc gtacgctttt ccgtttttcc agagtcccag ggtgatctgt ccctttttgg
      481 aaacggatgc ttctatgtgt tctgccttga cagtataaac ctctgtcccg tcctttttca
      541 gttcaaaaaa acgaacatct gtcggctgtg agttttcttc ctttttcgga gcatcttcgg
      601 tttccttctt cggaggactg gtgcattcaa taataaaaag agttataata ataaatttga
      661 tcagtttcat tttccctctc ccgcagtttc tgccccgaat tctgttttcg gaggagttct
      721 gtcgatcaga catctgaaag cctttactga agatgtattt cctaccttgt ccctggctct
      781 gactgcaatc ataagttcat cctgttctgt ctctgtgatc ctgatatcgc cgtcagtgag
      841 cgaccattca gtattctgaa atttgtactg aatattatcc acccccgcac cgtaatctgc
      901 agcatccaca tgaaacgtga cagggagctt gcagtagtcc ttccctttga atttcattac
      961 aggaaaataa ctcagatgga cttcaggagg agtagagtcc acattaaaga atactgttgc
     1021 ctgttccgag acattgccta cattgtcttc tgaaactgca gtgacagagt gttccccgtc
     1081 tttataaatt ctaagataat ctgcatagaa tacttcccag gactgcgagt ccacagaata
     1141 acggatttct ctcactcctg aatccttgtc cgaagcttcc agtctgaatt cggcgccggg
     1201 gctgaagtat ttttttccca tattttccgc atatctggaa tgaggtgcga gaatgatttt
     1261 cggacgagtc ttgtccacga agatttcttt tttctttgcc atttcaaggt tcccgagcat
     1321 atcccggctt ctgtagacaa gagtgttttt tccttcttct gtgactggaa tcggttcctt
     1381 gtatgtctgg aattcttttc cgttcaggct gaattcagta aatccagcgg aaacatcatc
     1441 tgtggacttc agagagaaag gcacctttcc gtctgtgaat atctgtccgt caaaagtgat
     1501 atacggattt tttttctctc gtactgccgg atacgctgta atgacttttt ccgggagtgc
     1561 cggttctgtt ttggcaggca ctgtgacag
//
LOCUS       DAOQGJ010001108         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58204_length_1588_cov_3.030007, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001108 DAOQGJ010000000
VERSION     DAOQGJ010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58204_length_1588_cov_3.030007"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1588)
                     /locus_tag="PK683_15505"
     CDS             complement(<1..>1588)
                     /locus_tag="PK683_15505"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HNH09901.1"
                     /translation="VVSLKNHQDGDYFLTSLVLNPLCANLNASEKIKTDFYIEQKKKF
                     QFKKYSSEIGGDLCISGQLNFRNSGSHILTVNADAMGKSMQGAGGAIVLGAALNSILR
                     TQEVIETDPLKWILNTVSELNSLFLAFNGSMLMSMFLGLISEETGRMLFVNAEHPHAV
                     LLRDGLSSFISESSVMRKLGTMENPDIRVSQINLFRGDTVICGSDGKDDIEILKENGK
                     KIMNEDENIFRYIAAESHGDLELIAEKLKKKGTLTDDLSLISISRNIPVWAPGIAMLF
                     IIIKFIMIYYHTEPNRRITGFLISGFFTFILAVHGTQDRLQFSSLFLCLLTGLKYIDI
                     SAHQRDRQYFALSATLLTGTVLLNSRDSVNIMLSILSIPVYWSLSTSGPIPVKRFLKI
                     GLLSFIPAFILMGLSIRVSSRLSLMSTGKARTGLGETVMPGNISELAMNRDPVLKLEA
                     DGRWNDVYLRSAVFDRTDGFNWFGSVQEARTAVFPMQKDFSGPTAKIYLRPVTDGWVP
                     TMENIYNVSKNAAFSGNADTG"
BASE COUNT          425 a          370 c          338 g          455 t
ORIGIN      
        1 atccggtgtc ggcatttccc gagaaagctg catttttgga tacattataa atattttcca
       61 ttgttggaac ccatccgtca gtgactgggc gcagataaat tttcgcagtg ggcccggaaa
      121 agtctttttg catcggaaat acagcagttc ttgcttcctg aactgaaccg aaccagttga
      181 atccgtcagt tctgtcaaag acagcgcttc tgaggtatac gtcattccat ctgccgtctg
      241 cttccagctt gagaacaggg tccctgttca ttgcaagttc actgatattt ccgggcatca
      301 ctgtttctcc aagacctgtt cttgcctttc ctgtgctcat caggctcagc ctgctgctca
      361 ctctgatgct cagccccatc agtataaatg cagggataaa tgaaagcaga cctatcttga
      421 gaaatctttt taccgggatg ggacctgacg ttgacagact ccagtagaca ggtatggaaa
      481 gaatggacag cattatattt actgaatctc tgctgttcag cagaactgtg cctgtcagaa
      541 gtgtcgcact gagtgcaaaa tactgcctgt ctctctgatg cgcggagatg tctatgtact
      601 tcagacctgt cagcaggcag aggaaaagag aagaaaactg cagacggtcc tgagttccat
      661 gcaccgccag tatgaaagtg aaaaaacctg aaatcagaaa tcctgtgatt cgcctgttcg
      721 gttcagtatg ataatatatc attataaatt ttataataat gaatagcatt gcaattcccg
      781 gagcccagac aggtatgttt ctggaaatgc tgatcagtga aagatcgtca gtcagagtgc
      841 cttttttttt cagtttttcc gcgatcagtt caagatcacc gtgagattcc gctgcaatat
      901 aacggaatat attttcatct tcattcatga tttttttacc gttttccttc agaatctcaa
      961 tatcgtcctt tccgtcagat ccgcagatca cagtatcccc ccgaaagaga ttgatctgag
     1021 aaaccctgat atccggattt tccatcgttc ccagttttct catcactgaa gactcgctga
     1081 tgaaggagga aagtccgtcc cgaagaagga ctgcatgagg atgttccgca ttgacgaaga
     1141 gcattctgcc tgtctcttca ctgatcagac cgaggaacat agacataagc atagatccgt
     1201 tgaatgccag aaaaagcgaa ttaagttcgc tcactgtatt cagtatccat ttaagaggat
     1261 cagtttctat cacttcctga gttctgagaa tgctgttcag agctgcacca agcacaatcg
     1321 ctcctccggc accctgcatg gactttccca tagcatctgc attgacagtc agaatatgcg
     1381 atccgctgtt tctgaaattc agctggccgg aaatgcagag atcccctccg atctccgagc
     1441 tgtacttctt gaactgaaat tttttcttct gctcaatgta aaaatcagtc tttatttttt
     1501 cggaggcatt caggtttgca cacagaggat tcagaacaag ggaggtcaga aaataatctc
     1561 cgtcctgatg attcttgagt gaaacaac
//
LOCUS       DAOQGJ010001109         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58211_length_1588_cov_2.735812, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001109 DAOQGJ010000000
VERSION     DAOQGJ010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58211_length_1588_cov_2.735812"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PK683_15510"
     CDS             complement(<1..803)
                     /locus_tag="PK683_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNH09902.1"
                     /translation="MNLSDYKPERITHIRKTIEIAGIIFQAEDFLSSAEILNLYSRNF
                     KRETSIEETSVKRSVHSIHSILGFEVIESKPSKGYRLKAEYKKNRVWMLPLLSQYLFF
                     IDGGSLNFSLESLPFQLESDSLYKLFIFNDARERKKPVEFTYIKYLENTVKKKRIQVY
                     SILLRGRKLFILGKDLNAGDIRHYIFTQVREILQVDEETDLAAISEKSFQDLYKDSLE
                     AFEGLSSRKVVLRFSKEAEIYIQKEYFHSSQRFFRNEENELFMEMNINYS"
     gene            1047..1256
                     /locus_tag="PK683_15515"
     CDS             1047..1256
                     /locus_tag="PK683_15515"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HNH09903.1"
                     /translation="MSIPPPHIHIKYGDYEAVMELNNLNIIQGSIPRKCRQMVREWAE
                     FNQQELLDSGTARTSILFPHWSNIH"
     gene            1283..1510
                     /locus_tag="PK683_15520"
     CDS             1283..1510
                     /locus_tag="PK683_15520"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09904.1"
                     /translation="MFYLGFLNGEEIETDLKNLIGKYVSLEDLHTAGINEEWGCLEFL
                     AGNADIEPMTLYKYAIQNRLTESEISLTSKN"
BASE COUNT          514 a          302 c          291 g          481 t
ORIGIN      
        1 gaataattaa tattcatttc cataaacagc tcgttctctt catttctaaa aaaacgctgc
       61 gacgaatgaa agtattcttt ctgtatataa atctccgctt cttttgaaaa ccgcaaaacg
      121 acctttctgg acgacaaccc ttcaaaagcc tccaaactgt ccttgtagag atcttgaaaa
      181 cttttttctg atatagcggc taaatcagtt tcttcgtcta cctggagtat ttctctgact
      241 tgagtgaaaa tataatgacg aatgtcgcct gcattcagat cttttcccag gatgaagagt
      301 tttcgacctc tcaaaagaat tgaatatact tgtattcttt ttttctttac agtattttcc
      361 aagtatttta tatacgtaaa ctcaacgggc ttttttctct ctcttgcatc attaaatata
      421 aataatttat atagcgaatc gctttccagc tggaaaggaa gagattctag agaaaaattc
      481 aatgagcctc cgtcaataaa gaagagatac tgagaaagca gaggaagcat ccataccctg
      541 ttctttttat attccgcctt cagacgatat ccctttgaag gtttcgactc aatgacttca
      601 aagcctagaa ttgaatgaat tgaatgcacg gaccgtttga ccgaagtctc ctcgatggac
      661 gtttctctct tgaagtttct ggagtataaa ttcagtattt cggcagaaga aagaaagtcc
      721 tctgcctgaa aaataattcc agcaatttca attgtttttc tgatatgggt aatcctttcc
      781 ggcttataat cggataaatt caaagataca gctcctttac tttactcaaa acatctttca
      841 gtaaccgcag tccattacca gccatttttg acactatgcc tcatctgcag atttgaaata
      901 tcattgacag atatgaatta taaatcagac tgcctctgca ggaatggaca actgtttcat
      961 ttaagtaagc atctgtttaa ggagaactgg aatgcctgaa atttcaagat tctatggaat
     1021 tttcatcaga atgtatttga ttgacagtga gtatcccccc gccccatatt catattaaat
     1081 atggggatta tgaagcagtg atggaactga ataatctgaa cattattcaa ggcagcatac
     1141 ccagaaaatg ccgtcagatg gtgagagaat gggcggaatt caatcagcag gaacttttgg
     1201 attctgggac agccagaact tccatactgt tcccccactg gagtaatata cactgaaaaa
     1261 atgcagacag atcagagaat atatttttta cttgggattt ttaaatggcg aagaaataga
     1321 aacggatttg aagaacctaa tcggaaaata tgtttcactg gaagatcttc atactgccgg
     1381 aataaatgag gaatggggat gcctggaatt tcttgccgga aatgcggaca ttgaaccaat
     1441 gactttatat aaatatgcaa tacagaatcg gctgactgaa tctgaaatct cattgacatc
     1501 caagaattaa aaaccggact gcaaggacat gaatttcgta gagacactta taaaatctga
     1561 gacagggttc gaaattgaca acggagat
//
LOCUS       DAOQGJ010001110         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58220_length_1588_cov_2.424005, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001110 DAOQGJ010000000
VERSION     DAOQGJ010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58220_length_1588_cov_2.424005"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..369)
                     /locus_tag="PK683_15525"
     CDS             complement(121..369)
                     /locus_tag="PK683_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09905.1"
                     /translation="MLKNKVLSPFRPGPVMTGEDLTLGDIDNLPKDLKILHNKLKLVN
                     NKYPDSGYTKNCANCSIAADKTISSGSRVPAKPADIGF"
     gene            513..809
                     /locus_tag="PK683_15530"
     CDS             513..809
                     /locus_tag="PK683_15530"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNH09906.1"
                     /translation="MKNLTSQSVKETLSALKPTLAEKYKIRKIGIFGSFADGTFNDTS
                     DIDILVEFDETPGWDFFQLNRELESVFHRNVDLITPDALKSIIKDNILNRVLFV"
     gene            806..1159
                     /locus_tag="PK683_15535"
     CDS             806..1159
                     /locus_tag="PK683_15535"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNH09907.1"
                     /translation="MKKEERNVSLYLTDMEEAIQNILNYTEGISFEEFKKSRMIQDAV
                     IRNFEVMGEAANRIPETVKNQYTEIPWADMYGLRNRIAHEYFGIDFGIIWKITKDFLP
                     DNLKRIQRIKSSLFI"
BASE COUNT          504 a          294 c          323 g          467 t
ORIGIN      
        1 tgtttatttt catagttaat ggcctgtttc tgatagtcat acattttttt ctgaactcct
       61 gaaaatccat cctctatagt cttaaactcc ctgcccggaa aagctttttc aatatcactt
      121 ctaaaaccct atatctgcag gcttggcagg aactctgctc ccactagaga tggtcttatc
      181 agctgcaatg gagcagttcg cacagttttt ggtataccca gaatcaggat atttattgtt
      241 caccaatttc agtttattat gcagtatttt taagtctttc ggaaggttgt ctatatcccc
      301 taaagttaaa tcctccccgg tcattaccgg acctggacgg aaaggagaga gaaccttgtt
      361 cttcagcaag tcgtagcctg aacttacgta agaggctgct ttcttaaatc cgctgacaat
      421 cggttcactg actgaacttt atttcatact gaatattttt cttgaatgtt ccgggttgaa
      481 taggagagtg tttaaagaga taatgcaggc ttatgaaaaa tctgacatca cagtccgtaa
      541 aagaaacact ttcagctctg aagcctactc tggctgaaaa gtacaaaatc agaaagattg
      601 gaatcttcgg ttcctttgcc gatgggacct ttaacgacac aagcgacatt gacatcctcg
      661 tggaattcga tgaaactccc ggctgggatt ttttccaact gaacagagaa ctggaaagtg
      721 tatttcatcg taatgtagat ttaattacac ctgatgcatt aaaatccatt ataaaagaca
      781 atatcctgaa cagggttctg tttgtatgaa gaaagaagaa aggaatgtct ccctgtatct
      841 gaccgatatg gaggaggcaa tacagaatat tctaaattat acagaaggta taagttttga
      901 agaattcaaa aagagcagaa tgattcagga tgcagtcatc agaaatttcg aagttatggg
      961 agaagcggca aaccggattc cggaaacagt aaaaaatcag tatactgaaa ttccgtgggc
     1021 agatatgtat ggactgagaa acagaatagc tcacgaatat tttggaatag actttggaat
     1081 tatatggaaa attacaaagg attttctgcc tgacaaccta aagagaatcc aaagaattaa
     1141 atcttcgctc tttatataaa agcatagaat ctgcatcaat gcctttttct tctgaatgta
     1201 tatatcgcat ccttgataaa acttctataa tctgcagacg tatataattg ctcggctgaa
     1261 atccctggtg gttccaattt tcggactatc ctgtttccag ccgatcagct tggacagtcc
     1321 gttaaatttc tgcagaagcg attccttgtc ctgaattcat ctaaaaagtg ctggctttcc
     1381 tgactggata aatttatttt ataggaattc acagtctgac tggaaggaat ctgattcaga
     1441 gcagagacag gatataatat gccggaaata gcaaatatca aagtaccgaa aagtttttca
     1501 gagtcgatag aacagctgaa gtccagactg atcacagcag tagatacagg aaatgtagaa
     1561 gatgtgaggg agattctgga agcagggg
//
LOCUS       DAOQGJ010001111         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58226_length_1588_cov_2.292890, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001111 DAOQGJ010000000
VERSION     DAOQGJ010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58226_length_1588_cov_2.292890"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /gene="tsaB"
                     /locus_tag="PK683_15540"
     CDS             <1..714
                     /gene="tsaB"
                     /locus_tag="PK683_15540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNH09908.1"
                     /translation="DSRKGRSSGVYSYLSQKKENTVQMKLVYLDTSTSFMVIGLYEIS
                     SEVSTVDILKKNAPRDAGQNLVPLTGQLLEKNGWEKPDLISAALGPGSFTGIRTAVSA
                     ARDFSQVWKIPLFGFDTLEVYCRYFSEKFSTDIVALLDGKMKKAFAGSVIQGIYSGTK
                     DIPPENLEAEFKMTENMKIATDMNLPVSHVSLYDDFPDASAVLRHRREELLSFPREPN
                     YEKVFPVYLRTTYAEGKFI"
     gene            complement(707..>1588)
                     /locus_tag="PK683_15545"
     CDS             complement(707..>1588)
                     /locus_tag="PK683_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH09909.1"
                     /translation="PLVFGTIGLFPCVVLDAVRAMDIIALPQTIHFGFMIFLISISVQ
                     LSEEMVEHYKNFQKQELELFKMEKAKTNFLFNVAEEFKSYLDESKKLIQSLTKENYSG
                     KELTDRLLNMESLGGLKKAMIHDAELLHSVESRQFEYFTERFSLREMINEILTMLETR
                     HRQKRNSLSVSFQQDIDMQQSKELVFHVIYHILENIFLYTPEESPASVNVSLLGKTVE
                     LKFRDSGPGMNPEEAANAFKKFYRAPSSEEKKIHGSGIGLTLVRSIAVLLAGETSLES
                     EEGAGTTVTVLIPQSVI"
BASE COUNT          417 a          350 c          354 g          467 t
ORIGIN      
        1 gattcccgaa aaggccgttc atctggagtt tactcatatc tctcccagaa aaaggaaaat
       61 actgttcaaa tgaaacttgt atatctggat acttccactt cttttatggt catcggtctg
      121 tacgaaattt cttccgaagt gagcacagtg gatatactta aaaaaaatgc acccagagat
      181 gcaggtcaga atcttgttcc tctgactgga cagcttcttg aaaaaaacgg gtgggaaaaa
      241 cctgatctga tttccgcggc actcggtcct ggttccttta ctgggatcag aacggcagtc
      301 agcgccgcac gtgatttttc acaggtatgg aagattcctc ttttcggatt tgacactctc
      361 gaagtgtact gcagatattt cagcgaaaag ttcagtacag atattgtcgc cttgcttgac
      421 ggtaaaatga agaaggcatt tgccggttct gtcattcagg gcatttactc aggcacaaag
      481 gatattccgc ctgaaaatct ggaggcagaa ttcaagatga ctgagaatat gaaaattgca
      541 actgatatga atctgcccgt atctcacgtc agtctgtacg acgattttcc tgacgcttca
      601 gcagtattga gacacaggcg ggaagagctg ctttccttcc ccagggagcc taattatgaa
      661 aaggtgtttc ccgtttacct gagaaccacc tatgcagagg gaaaatttat atgacggact
      721 gaggaatcag aaccgtcaca gtcgttcctg ctccttcttc ggattccagt gaggtttccc
      781 ctgcaagcag aacagcgatt gaacggacga gtgtcagacc gattccgctt ccgtgaattt
      841 ttttctcttc ggaagaaggt gctctgtaga atttcttgaa agcatttgcc gcctcttccg
      901 gattcattcc ggggccggaa tctctgaact tcagttcaac agtctttccc agaagtgaaa
      961 cgttcacgga tgccggactt tcctccggtg tatataaaaa aatgttttcc aggatatgat
     1021 atatcacatg aaacacgagt tccttgctct gctgcatatc gatatcctgc tgaaatgaga
     1081 cgctcagact gtttcttttc tgtctgtgtc tggtctcaag cattgtgaga atttcattga
     1141 tcatctccct cagtgaaaat ctctcggtaa agtattcaaa ctgtctggac tcgactgagt
     1201 gaagaagctc tgcatcgtga atcattgcct ttttcagtcc tccaagactt tccatattca
     1261 gcagtctgtc ggtcagttcc tttcctgaat agttttcctt tgtcagcgac tggatgagtt
     1321 ttttggattc atcaagatag gacttgaatt cttccgctac attgaagagg aagtttgtct
     1381 tggctttttc cattttgaaa agttccagtt cctgcttctg gaagttttta tagtgttcta
     1441 ccatctcttc tgagagctgg acggagatgc tgatgaggaa aatcatgaac ccgaaatgaa
     1501 ttgtctgcgg gagagcaata atgtccattg cacggactgc atcgagaacc acgcaaggaa
     1561 aaagacctat cgttccgaat acaagagg
//
LOCUS       DAOQGJ010001112         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58250_length_1587_cov_4.334856, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001112 DAOQGJ010000000
VERSION     DAOQGJ010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58250_length_1587_cov_4.334856"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PK683_15550"
     CDS             complement(<1..1058)
                     /locus_tag="PK683_15550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH09910.1"
                     /translation="MLECNTKDFYDPAQTKITFYGDSRMDFVNSPGYGNSTMPSILNE
                     PFGSAGAYSADSLFSEESIQNLGKEGYRSYEIVRHLDNCIPNMSKNYKIAKRFVFHVG
                     GNNFMDSLAASWYAVNHLPKTAYGRSILPAVLAIYFEVTRFNLRRDLEYIIMNLAAIS
                     SKTKNGKPDVLVVGGYPARGRFLNVTLDQTPQIIPGVGNPFADEVKMTDPLAFYLSIG
                     EYFTVGLNSMEADYSGLAAKYNAEYVRVWDEMNKPQEYMALDLIHPNAAGFKKWGSVV
                     GKKLREISFNSAPKARSFTSTVGPTVNVSGSCPVSGTFSVTGTATTSSGTTTTNLSYV
                     LNSCKETITFSTMTGSVA"
     gene            complement(1145..>1587)
                     /locus_tag="PK683_15555"
     CDS             complement(1145..>1587)
                     /locus_tag="PK683_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09911.1"
                     /translation="DLMILYFFTDGASIMNNCDTRYKYKIVNFDQIITGKGQGNTFRI
                     YGLIDGSQLVLKNDSSDTACEVYAILTPCDALNNEASFSEVYKQICTNIQTYTFSIYP
                     SDKEKRCSFNSDLLSRYQSSIIFILGAVSPNGSTNCSFSGAITK"
BASE COUNT          485 a          323 c          304 g          475 t
ORIGIN      
        1 aaggccactg agcctgtcat tgtagaaaag gtgattgtct ccttgcagga attcagcaca
       61 taagacaggt tcgtcgtcgt tgttcccgaa gaagttgtcg cagttcccgt cacggaaaaa
      121 gtaccggaaa caggacagga gccggagaca ttcacagtcg gtccgactgt agatgtgaaa
      181 gaacgggctt tgggtgcaga attgaaagat atttcacgga gttttttacc tacgacactt
      241 ccccattttt taaaacctgc agcattaggg tgaatcagat caagtgccat atattcctgc
      301 ggtttgttca tttcgtccca gacgcgtaca tactctgcgt tatactttgc cgctaatccg
      361 ctatagtctg cttccatgga attcaaccct acagtgaagt attctccgat agaaagatag
      421 aaagccaaag ggtctgtcat tttcacttca tctgcaaaag gattgcctac ccccggaata
      481 atttgaggag tctgatccaa tgtaacattt aagaatcgtc ctcttgcagg ataaccccct
      541 acaacaagta catccggctt gccatttttt gtcttgctgg atattgctgc cagattcatg
      601 atgatatatt ccagatcccg tctcagattg aatcttgtta cttcaaaata aatcgcgagg
      661 acagccggta aaattgacct tccatacgct gttttgggaa gatgattgac tgcataccag
      721 gatgcagcca gagaatccat aaaattattt cctcccacat gaaaaacaaa acgctttgca
      781 attttataat ttttggacat attagggata cagttatcaa gatgacggac gatttcatag
      841 gaacgatatc cttcttttcc taaattctga atgctctcct cagaaaacag agaatctgca
      901 gaataagctc cggcacttcc aaatggttca ttcaggatag agggcattgt gctgtttccg
      961 tatccaggac tgtttacaaa atccatccgg ctgtcaccgt aaaatgtgat tttggtctgg
     1021 gcaggatcat aaaaatcttt agtattacat tcaagcataa tcggttgacg accatcacct
     1081 tcataagctt gtaaagagac gaaaatcccg gaaataaaaa gaaaaaagga aagtttaaaa
     1141 aaaatcattt tgtgatagcc cccgaaaaac tacaattagt tgaaccattt ggactgactg
     1201 ctcctagtat aaagattata gaactttgat atctgctcag gagatcagaa ttgaatgaac
     1261 atcgtttttc cttgtctgag gggtaaatgc taaatgtgta tgtttggata ttggtgcaga
     1321 tttgcttata cacctcagaa aaagatgctt cattgttaag agcatcacat ggagtaagga
     1381 ttgcatatac ctcacaagct gtgtctgagc tatcattttt caatactaac tgacttccat
     1441 caatcagtcc ataaattcgg aaagtatttc cttgaccttt tccagtaatt atttgatcaa
     1501 aattaactat tttatatttg tatcttgtat cgcaattatt cattattgat gccccgtctg
     1561 taaaaaaata caatatcatc aaatctt
//
LOCUS       DAOQGJ010001113         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58292_length_1587_cov_2.369452, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001113 DAOQGJ010000000
VERSION     DAOQGJ010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58292_length_1587_cov_2.369452"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..>1587
                     /locus_tag="PK683_15560"
     CDS             22..>1587
                     /locus_tag="PK683_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09912.1"
                     /translation="MFTMKSFIIFLLAAVSIFSENLSPLKKFHFKSRESKILPGNSGE
                     KAKGFNCSGTVNPEDQAGDFALLSSFYQNGCYTDGRYNSSNFKVDLGTAETSDGKTKY
                     PAVRIHPPEKKAQDFLASHHNVYYKSHDKNEVNIYHLSDEGVMTKSFKTNAGITASGF
                     QKAEGFLVSQISSPQVYVFMPVSEKFSLFQTLSKDAEGAKVFDEKGIELDRYRFSIQG
                     NIYDTANGFTAGPESHSAVYGRNRQFFTSPLPNGIAVIWQDEKTDDVYASILSEKLDS
                     HRQVKLSSTANYQLAAATADPSGNIYHMLIEKPAKSFSARKVSVFKSSADGKALKTAS
                     LDASEKGLNITKFGEDTIASMVYSNEKLGMIISRTMHKSSDGLNHQGAIGIVLDAKTL
                     TLLHNHGQTSGHSFGNILDRDSEGKFLGIDLGDNYPRGVHLHRFDEKSRSSRVVYTFK
                     TEHGDRAMSPAGRNYPVYREISGNGKTYYQWSNDNRTYTELAGIHETQSGIAVFFAGE
                     PDSSGKAINNSRAQ"
BASE COUNT          516 a          336 c          360 g          375 t
ORIGIN      
        1 actgtcaggg aaggtataaa aatgttcact atgaaaagct tcatcatttt tctgcttgcg
       61 gcagtttcga ttttctctga aaacctttct cctctgaaaa aatttcactt caaaagcagg
      121 gagtctaaaa ttcttccggg gaattcagga gagaaggcaa aaggattcaa ctgctcaggc
      181 acagtaaacc ccgaagacca ggctggagat tttgcgcttc tgtcctcctt ttaccagaac
      241 ggatgttata cggacggaag gtacaacagc agcaacttca aggtggatct gggaactgca
      301 gaaacttccg atgggaagac aaaatatccg gcagtaagaa ttcatccacc ggaaaagaaa
      361 gctcaggatt ttctggcatc acatcataat gtttattata aaagtcatga taaaaatgaa
      421 gtgaatatat atcatctgag tgatgagggt gtcatgacaa aatcattcaa gacaaatgca
      481 ggaatcactg cttcaggatt tcagaaagca gaaggatttc tggtcagtca gatttcatct
      541 ccgcaggttt atgtatttat gcctgtttcc gagaagttca gcctttttca gacactgagc
      601 aaagatgcgg aaggagcaaa agtctttgat gaaaaaggga tagaattgga cagatacaga
      661 ttttcgattc agggaaatat ctatgacacg gcaaacggat ttacagcagg acctgagagt
      721 cacagcgcag tctacggaag aaacagacag ttttttactt cgccgctccc gaacggaatt
      781 gcagtcatct ggcaggatga aaaaacagat gatgtctatg caagcatcct cagcgaaaaa
      841 ctggactctc acagacaggt aaaactttcc tcaactgcaa attaccagct ggctgcggcg
      901 acagcagatc cttccggaaa catctatcat atgctgattg aaaaacctgc caaaagtttt
      961 tctgcaagaa aggtcagtgt cttcaaatca agtgcagacg gaaaagccct gaaaacagca
     1021 tcactcgatg cctcggaaaa aggactgaac atcactaaat tcggagagga cacaattgct
     1081 tccatggtgt acagcaatga aaagcttggg atgatcattt caagaacaat gcacaagtct
     1141 tcggacggac tcaatcatca gggagcaata gggattgtgc tggatgctaa aaccctcacc
     1201 cttctgcaca accacggtca gacttccgga cattccttcg ggaacattct ggacagagat
     1261 tcagaaggaa aatttttagg aatagatctt ggagacaatt accccagagg agtccacctg
     1321 caccgctttg atgaaaagtc cagatccagc cgtgtcgtat acacattcaa gacagaacat
     1381 ggagacaggg caatgtctcc tgcaggaaga aattatcctg tctacaggga aatcagtgga
     1441 aatgggaaga cttattatca gtggtcaaat gataaccgca cttatacaga gcttgcaggc
     1501 atccatgaaa cgcagagcgg aattgctgta ttctttgcag gagaaccgga ctcttccggg
     1561 aaggcaatca acaattcccg tgcacag
//
LOCUS       DAOQGJ010001114         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58300_length_1587_cov_2.055483, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001114 DAOQGJ010000000
VERSION     DAOQGJ010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58300_length_1587_cov_2.055483"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..856)
                     /locus_tag="PK683_15565"
     CDS             complement(59..856)
                     /locus_tag="PK683_15565"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNH09913.1"
                     /translation="MKLPYIQRAVGIFLISAALILTAALFYMIREKRIFESKLHFYTL
                     LDRGDGITSGTPVMYKGIPAGNVTEASLNEEGKISVTFEMFPEFKKHLKGFSFVKIGS
                     ASLLGGKILDMVTSDTGDILPNWAYVPSNDDPAIKAYLSSPEYRKQHQDDQNTKIANI
                     LNNVEVITGNLKNISSDFRNPESGISSALNNVKRITSNMAQITGEIKSASPEIRQSIQ
                     DTQSTIENVNKIVKSARKGPLIRMIGKEEETESSAPLFDMDPRDSGR"
     gene            complement(856..1539)
                     /locus_tag="PK683_15570"
     CDS             complement(856..1539)
                     /locus_tag="PK683_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH09914.1"
                     /translation="MSLFRAENVSLNSVMDGAVFENFNITVREGEKVIIRGTLSERKL
                     LLQILSGQAEPVKGKIFLQDEEVKYFINTEYFEKRKIISVISGTLSPLANLTVRENLE
                     LPLRFDRTVSEEEREDITENMLEILNLKSKAGLRPDRLNSEEKKLLTAAAGLKSTTKL
                     ILADDTLEKMDEDSLQMLKWLINYRIDTDSTAAVFFFRKKVLFSDEKFTSYITENGKL
                     ERILSLEED"
BASE COUNT          410 a          340 c          301 g          536 t
ORIGIN      
        1 atcagaaaaa aattaatctt cttcagcaga cgcagtccct gggaaaggta tgttatcatt
       61 atctgccgga atctctcgga tccatgtcaa aaagaggagc agaggattct gtctcttctt
      121 cctttcctat catcctgata agaggtcctt ttcttgcgga tttcacaatc ttattcacat
      181 tttcaattgt ggactgtgtg tcctgaatcg actgcctgat ttccggagat gccgatttaa
      241 tttctcctgt gatctgcgcc atattagatg tgattctttt cacattgttc aatgcagaag
      301 aaatcccgct ttccggattt ctgaaatcag aactgatgtt tttcagattt cctgtaatga
      361 cttccacatt gttgaggata tttgcaatct ttgtgttctg gtcatcctgg tgctgctttc
      421 tatattcagg agaggagaga taagccttta ttgcagggtc atcattcgaa ggtacatatg
      481 cccagttggg aagaatgtcg ccggtgtccg aagtgaccat atccagaatt tttcctccaa
      541 gcagggaagc tgatccgatt tttacaaaac tgaagccttt cagatgtttt ttgaattcag
      601 gaaacatttc aaatgtcaca gatatctttc cctcttcatt gagagatgct tctgtcacat
      661 ttcctgccgg tattcctttg tacatgactg gagttcctga cgttattccg tcccctcggt
      721 ccagaagggt gtaaaaatga agcttggatt caaatattct cttctctctg atcatataaa
      781 acagcgccgc tgtgagtata agtgctgcag agatcaggaa tattcccact gctctctgta
      841 tataagggag tttcatcagt cttcctccag tgaaagaatt ctttctagtt ttccgttctc
      901 agtgatataa cttgtgaatt tttcatcact gaaaagaact ttttttctga aaaagaatac
      961 agccgcagtg ctgtctgtat caatcctgta attgatgagc catttcagca tctgcagtga
     1021 gtcttcatcc atcttttcga gtgtatcgtc tgcgagaatg agttttgtcg tacttttcag
     1081 acctgcagct gcagtcagaa gttttttttc ttcactgttc agtctgtctg gtcttagccc
     1141 tgcttttgat ttcagattca gtatctcgag catattttca gttatgtcct ctctttcttc
     1201 ttctgaaact gttctgtcaa accgaagggg cagttcaaga ttttcccgga cagtcagatt
     1261 tgccagagga gagagtgttc ctgaaataac ggatataatt ttcctttttt caaaatactc
     1321 cgtattgata aaatatttta cttcctcatc ctgaaggaaa atttttcctt taacaggttc
     1381 agcctgtcca gataaaatct gaaggagaag ttttctttcg gacagagttc ctctgatgat
     1441 cactttttca ccttcccgaa ccgtgatgtt gaagttttca aatacagccc cgtccataac
     1501 tgaattcagc gaaacattct ctgctctaaa caggctcaca gataaagtac cgccgcaaaa
     1561 aaaatatcaa tcagtataac atacaga
//
LOCUS       DAOQGJ010001115         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58337_length_1586_cov_3.489876, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001115 DAOQGJ010000000
VERSION     DAOQGJ010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58337_length_1586_cov_3.489876"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PK683_15575"
     CDS             <1..822
                     /locus_tag="PK683_15575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH09915.1"
                     /translation="PDNISGDPFRLKQILLNLLSNSVKFTEAGEIRFETRLISEENES
                     FRLEFTVTDTGIGMNDSEIRKIFEPFVQADSSTTRKYGGTGLGLVIVRRLTESMGGKT
                     SVESEKGKGSIFRLEIPFRKASAVNDISIYEPLKKEKLYDLYPFRILLVEDNRANQKL
                     IERILEKEGYKIITADNGQICLDVMETEEADLIFMDYQMPVMDGPTASAEIRKKHGKE
                     IIIIALTANVLEEDRKICRDSGMDDFISKPARPYDLREKISHWGQKKFNSENQNI"
     gene            complement(826..>1586)
                     /locus_tag="PK683_15580"
     CDS             complement(826..>1586)
                     /locus_tag="PK683_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002177115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09916.1"
                     /translation="RLPWNHVVKIGKSKLLSDPLFGTLYKKVGNELESYLKPEEMQLK
                     NFLQKKAGSTESDRIEYNFRRLGLERFHYWKIKTLLPDLVLPTRHFMGLRFRTGLFSY
                     PIMTMTPCDNQLLIPGKHLKEFVELKEKQKQGFFEDTVLPSWKLNFNYQRFGEVSVSK
                     LKKIAYNFHRVIEATKYLAEKEKLILDLHSENIIIVLPEFELKIFDFHLFDEHLYDFG
                     DGTASPVEEHIQTIQTFINSFELSRKDLSEDQTE"
BASE COUNT          503 a          305 c          340 g          438 t
ORIGIN      
        1 ccggataata tttcaggtga tccttttcgg ctgaagcaga tccttctgaa tctgctgagc
       61 aattcagtca aattcacgga ggccggtgaa atccgctttg aaaccagact catcagtgaa
      121 gaaaacgaat cattcaggct tgaatttact gtaactgata ccggtatcgg aatgaatgat
      181 tctgaaatca ggaagatatt tgagcctttt gtacaggcgg attcctccac tacgagaaaa
      241 tacggcggaa ccggccttgg actggtgatt gtaagaagac ttactgaaag tatgggaggg
      301 aaaacctccg ttgaaagcga gaagggaaaa ggaagcatat tccgtctgga aataccgttc
      361 agaaaagctt ctgcagtgaa tgatataagc atttatgaac ctcttaaaaa ggagaaactt
      421 tatgatctct atccgttcag gatacttctg gttgaggaca acagagcaaa tcagaaactg
      481 atagaaagaa ttcttgaaaa agaaggatat aaaataatta ccgccgacaa tggacagatc
      541 tgcctggatg taatggaaac agaggaagca gatctcatat ttatggacta tcagatgcct
      601 gtaatggacg gacctacagc gtctgcagag ataagaaaaa aacacggcaa ggaaattatt
      661 attattgcac tgactgcaaa tgtgcttgaa gaggacagaa aaatctgcag agattccgga
      721 atggatgatt ttatatcaaa gccggccaga ccttatgatc tcagggaaaa aatctctcac
      781 tggggtcaga aaaaattcaa ttcagaaaat cagaatatct gatttctact cagtctgatc
      841 ctctgaaaga tcctttctgc tgagttcaaa tgaattaata aaagtctgta ttgtctgtat
      901 atgttcttcc actggagatg cagtaccgtc accgaagtca taaagatgtt cgtcgaacag
      961 atgaaaatcg aatatcttga gctcaaattc aggcagaaca atgatgatgt tttcagaatg
     1021 cagatccaga atcagttttt ccttttctgc aagatatttt gtagcctcaa tcactctgtg
     1081 aaaattatac gcaatcttct tcagtttgga tacggaaacc tcgccgaatc tctgataatt
     1141 aaaatttaat ttccagctcg gaagaacagt atcttcaaaa aagccctgct tctgcttctc
     1201 cttgagctcc acaaattcct tcagatgttt tcccggaatc agaagctgat tgtcgcaggg
     1261 tgtcattgtc ataataggat aggaaaagag acctgttctg aacctgaggc ccataaaatg
     1321 ccgtgtagga aggacaagat cagggagcag agtctttatt ttccagtaat gaaaccgttc
     1381 cagtccaagg cgtctgaaat tatattcaat cctgtctgat tcagtgctcc ctgccttttt
     1441 ctgcagaaag tttttcagct gcatttcttc aggcttgaga taactctcaa gttcattgcc
     1501 tactttcttg taaagtgtgc cgaaaagagg atctgaaaga agttttgatt tcccgatttt
     1561 gaccacatgg ttccagggca gacggt
//
LOCUS       DAOQGJ010001116         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58399_length_1585_cov_4.228105, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001116 DAOQGJ010000000
VERSION     DAOQGJ010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58399_length_1585_cov_4.228105"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /gene="cysT"
                     /locus_tag="PK683_15585"
     CDS             complement(<1..626)
                     /gene="cysT"
                     /locus_tag="PK683_15585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNH09917.1"
                     /translation="MFSRKNDILPGFRPTMAYTLFYLALIVLIPLGALLYNGSKAGFE
                     GFTNVMKEERVLSAVYVSFGTALTASAINCIFGLVIAWCIARYSFPGKEILDTLIDLP
                     LTLPTAVAGISLTAIYAETGFMGKFLAKFGIKAVFNFTGITIALMFIGLPFIVRTVQP
                     VIMELSKEDEEAASSLGATKFQTFRFVLFPVILPSLISGFGMAFSRCLG"
     gene            complement(638..>1585)
                     /locus_tag="PK683_15590"
     CDS             complement(638..>1585)
                     /locus_tag="PK683_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH09918.1"
                     /translation="TLLNVSYDPTREFFKEYNEEFAKVWKTKTGTDIKINQSHGGSGK
                     QARSVIDGLEADVVTLALSYDINEIASKGLIDAGWESAFPHRSTPFTSTIVFVVRKGN
                     PKNIKDWDDLVKPGIGVITPNPKTSGGARWNYLAAWAYASKKIGNDENKVKDFIKKLY
                     NNVPVLDAGARGSTTSFAQRKLGDVLISWENEAFLIMDGPGNDQFEKVIPSESILAEP
                     PVAVVEKSAAKRNTAEAAKFYLEQLYSQNAQEIAAKHGYRPRNEEIMKKYSDKFPKLS
                     LTDISFFGGWNKAQKDHFADGGNFDQIYTKSAKDKQENK"
BASE COUNT          398 a          369 c          332 g          486 t
ORIGIN      
        1 ccgaggcatc tcgaaaatgc cattccgaat cccgaaatca gcgaaggaag aatgactgga
       61 aaaagaacaa accggaatgt ctgaaatttg gtcgctccca gactggacgc ggcttcctca
      121 tcctccttgc tcagttccat aatcacaggc tggacggttc ttacaatgaa cggaagtccg
      181 atgaacataa gtgcaattgt aattccagtg aaattgaaca cagcctttat tccgaacttt
      241 gcaagaaatt ttcccataaa acctgtctct gcgtaaatag cagtcaggct gattccagca
      301 actgctgtag gcagagtcag agggagatcg atcagagtgt cgagtatttc ttttccaggg
      361 aaactgtatc tggctatgca ccaggctatg acaagtccga aaatgcagtt gattgcagat
      421 gcagtcagtg ccgtcccgaa gcttacatat actgcagaaa gaacccgttc ttccttcatt
      481 acgtttgtaa aaccttcaaa tcctgccttg cttccgttgt aaagaagagc tccaagagga
      541 atcagcacaa tcagtgccag atagaagagt gtgtatgcca ttgtaggcct gaatccggga
      601 aggatgtcat tttttctgct gaacattttt tataagctca cttattttcc tgcttatctt
      661 tggcagattt tgtgtaaatc tgatcaaagt ttccgccgtc tgcaaaatga tctttctgtg
      721 ctttgttcca tccgccgaaa aagctgatat cagtcagact gagtttggga aatttgtccg
      781 aatatttttt catgatttct tcatttctag gcctgtatcc gtgttttgct gcgatttcct
      841 gtgcattctg cgaatacagc tgttccagat aaaactttgc agcttccgct gtatttcttt
      901 ttgcggccga tttttccacg accgcaacag gaggctctgc aaggatactt tcactgggaa
      961 tcactttctc aaactggtca tttcccgggc cgtccataat caggaaggct tcattttccc
     1021 aggaaatgag aacgtctcca agttttctct gcgcaaaact tgtagtagag cctctcgctc
     1081 ccgcatccag aacaggaaca ttattgtata actttttgat aaaatctttg actttattct
     1141 catcattccc gatttttttt gaagcgtatg cccaggcagc aagataattc catcttgctc
     1201 ctccgcttgt ctttggattt ggagtgatga ctccgatacc cggtttgaca agatcatccc
     1261 agtctttgat gttcttagga tttccttttc tgactacaaa tacaattgtg gaagtgaaag
     1321 gtgtgcttct gtgcgggaag gcggattccc agccggcatc gattagacct ttggatgcga
     1381 tttcgttaat gtcgtatgaa agagccaggg tcactacatc agcttccagt ccgtcgatta
     1441 cacttctggc ctgcttgccg cttcccccat gcgactgatt tattttgata tcagttccgg
     1501 tcttcgtctt ccatactttt gcgaactctt cgttgtactc cttgaaaaac tctctggtcg
     1561 gatcataaga aacattcaga agagt
//
LOCUS       DAOQGJ010001117         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58403_length_1585_cov_3.646405, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001117 DAOQGJ010000000
VERSION     DAOQGJ010001117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58403_length_1585_cov_3.646405"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1585)
                     /locus_tag="PK683_15595"
     CDS             complement(<1..>1585)
                     /locus_tag="PK683_15595"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNH09919.1"
                     /translation="KGEKTGFYDINTDKIIADIIYDKDSFGTGFAETDRKEYYLAEVC
                     RGGKCALIDSKGKELTEFKYDRIEVHNIRYGHISADAGKKQAVLNLKGEEVIPAVYSL
                     ISAPMEGVSSVTVKNDKGLFGVYDLEKRKETVPCIYEQIQIFNSGYQYAVQKAGKWGL
                     MSSDGVLLVKIHYDEIQYKEYGIYAVRIGEKEGFYSSAKKKMISEVRFDRYSSFYGFK
                     ETPKKGYAVTEVFLKEKGALMDSTGKILTEFKYGRIDSLNYRSGYFFAEIKKKQGLLK
                     YDGTETVPVVYSNISVPSEKHSLVEIQKGKKSGVFDLKKGEEIIPCDYSQIQISEDGK
                     YIFALQKKKWGVLSLKNETVIPFEYIELRRHHQGMFSVKGKDGWKFVNRKNVQVGRYK
                     YEDIDYRYSGQGWDKLSLNTILVKRTGLWGTVDEKGEELIVPKYDKDFVFYEDRCGTA
                     DTALAGHPVFINTKGEVIAAQKEPYEEVTGFSRDCTARAKIKGKYGVINKEGKIIIPF
                     QYENLGVNQDYWFGMDYYAS"
BASE COUNT          409 a          390 c          250 g          536 t
ORIGIN      
        1 ggatgcataa taatccattc cgaaccagta gtcctgattt actcccagat tttcatactg
       61 aaatggaata atgatttttc cttctttatt aatgactcca tattttcctt ttattttagc
      121 cctggcagtg cagtctctgg aaaatccggt gacttcctca taaggttctt tctgcgctgc
      181 gatgacctca cccttcgtat taataaacac aggatgaccg gctagagcag tatccgcagt
      241 tccgcagcgg tcctcataaa atacaaagtc cttatcatac ttcggaacaa tgagttcttc
      301 tcctttctca tcgactgtgc cccataaccc ggttctcttc acaaggattg tatttaatga
      361 gagtttatcc cagccttgtc ccgaatatct gtaatcaata tcctcatact tgtatctgcc
      421 gacctgaaca tttttcctgt tcacaaattt ccagccgtct tttcctttga ccgaaaacat
      481 accctgatga tgccttctca gttcaatata ttcgaaagga atgactgttt cattctttaa
      541 agaaagaact ccccattttt tcttctgaag ggcaaaaata tatttcccat cctcagaaat
      601 ttgaatctgg gaataatcgc agggaatgat ctcttccccc ttcttcagat cgaatactcc
      661 ggactttttg cctttctgga tttccaccag ggaatgcttt tctgaaggaa cagatatgtt
      721 tgaatacaca actggaacag tttcggtccc gtcatatttc aaaagtccct gttttttttt
      781 gatctctgca aaaaagtatc ctgaacggta attcagactg tcaatccttc catatttgaa
      841 ctctgtcaga attttcccag tgctgtccat caaagctccc ttttccttca gaaacacttc
      901 ggtgactgca tatccttttt tgggagtctc cttgaaaccg taaaaagaac tgtatctgtc
      961 gaatctgact tctgaaatca tcttcttttt tgcagaactg taaaaacctt ccttttcccc
     1021 aattcgaact gcatatatcc catattcttt atattgtatc tcatcataat gaatttttac
     1081 aagaagaact ccatcggaag acattaggcc ccatttacct gccttctgaa ctgcatactg
     1141 gtatcctgaa ttgaaaattt ggatctgctc gtaaatgcag ggaacagtct cctttctttt
     1201 ctccagatca tacacaccga acagtccttt gtcattcttt actgttacag aggaaactcc
     1261 ttccataggt gctgaaatca gagaatagac tgcagggatc acctcttctc ctttaaggtt
     1321 cagaacagcc tgttttttac cggcatcggc ggaaatatgt ccataacgga tattgtggac
     1381 ttctattctg tcatacttga attcggtcag ttcctttcct ttgctgtcga tcagtgcaca
     1441 cttccctccc ctgcagactt ctgccaggta atactctttc ctgtccgttt cagcaaaccc
     1501 tgtcccaaaa ctgtctttgt catatataat atctgcaatt attttatcag tattgatatc
     1561 gtaaaaacct gtcttttcgc ctttc
//
LOCUS       DAOQGJ010001118         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58410_length_1585_cov_3.396078, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001118 DAOQGJ010000000
VERSION     DAOQGJ010001118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58410_length_1585_cov_3.396078"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PK683_15600"
     CDS             complement(<1..418)
                     /locus_tag="PK683_15600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH09920.1"
                     /translation="MIRNRKVFFILCTLLSIGAFASDVKFSWPIQTSALENKLTSLFG
                     ESRGDHFHNGVDISSDNEEIRPIDQGQILFSRFDSDNPFRNGMGSGNCVWIGHSKGFL
                     SGYFHLKDGREDGLLKKVQVNKTDVIGRSGNTGHSMG"
     gene            complement(415..1359)
                     /gene="ispH"
                     /locus_tag="PK683_15605"
     CDS             complement(415..1359)
                     /gene="ispH"
                     /locus_tag="PK683_15605"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNH09921.1"
                     /translation="MALEKIYLANPRGFCAGVKYAISYVYNLQEKFPEEDIYVRKEIV
                     HNQRVVTDMKKRGIKFIQELNEAPENSIVVFSAHGVSPSIVDYARDNKMRIGDATCPL
                     VTRVHRKARKLKDTHQIIYIGHEGHDEAIGTMGEAEMFLAETEEDILNLKDKINPEKP
                     LTYLMQTTLSVHDTRKIVEKIQEIFPFVEHPDKDDICYATTERQEAVSQMLDNIDAML
                     VIGASNSSNSLRLLELAQKKKKHSFRISSREELEKEKLDSLDIRVLGITAGASSPQIL
                     VEEIIEKLKLLYPSVSVENYPESREDSMLFKLPKELLK"
     gene            complement(1359..>1585)
                     /locus_tag="PK683_15610"
     CDS             complement(1359..>1585)
                     /locus_tag="PK683_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNH09922.1"
                     /translation="VVLILLGKLGIISGRFLAEKWREATIIISIFSAVFSPGPDVTFM
                     LMLFVPLEILFLISVFIMKVSEKNRDPGGV"
BASE COUNT          442 a          379 c          306 g          458 t
ORIGIN      
        1 agcccatgga gtgaccagta tttccggacc tgccgatcac atccgttttg ttcacctgaa
       61 ccttcttcag aagaccgtct tcccgaccgt ccttcaagtg aaaatagccg gatagaaagc
      121 ccttggaatg tccgatccaa acgcagtttc cgcttcccat tccgtttcgg aaaggattgt
      181 ctgaatcaaa tctggagaaa agaatctgtc cctgatcgat tggacggatt tcctcattgt
      241 cggaagaaat atccactccg ttgtgaaaat gatcccccct ggattccccg aacagagaag
      301 tcagcttatt ctcgagtgcc gatgtctgaa tgggccagga aaattttaca tcagaagcaa
      361 atgctcctat ggaaagaagt gtgcacagaa taaaaaaaac ttttcgattt ctaatcattt
      421 cagaagttcc tttgggagtt taaagagcat agaatcttct ctcgattcag gataattctc
      481 gacagaaacg gaaggataaa gcagcttcag cttttcaatg atttcttcca cgagaatctg
      541 aggactggat gcgcctgcag taattccgag aacacgtata tcgagtgaat ccagtttttc
      601 cttttccagt tcttccctgg aactgattcg gaacgagtgt ttcttttttt tctgagccag
      661 ttccagaagt ctgagagaat ttgaactgtt ggatgcaccg atgacaagca tcgcatcaat
      721 attgtccagc atctgcgaaa cagcttcctg cctttccgtc gtagcatagc agatatcatc
      781 cttgtcagga tgttccacaa agggaaatat ctcctgaatt ttctccacaa tcttccttgt
      841 atcatgaaca gaaagcgtgg tctgcatcag ataggtcaga ggtttttcag gattgatctt
      901 atccttcaga ttcagaatat cttcttctgt ttctgcaaga aacatctccg cctcacccat
      961 tgtaccgatt gcttcatcgt gcccttcatg gccgatatat atgatctgat gagtgtcctt
     1021 cagttttctt gcctttctgt ggacccgagt cacaagagga caggtggcat caccgattct
     1081 cattttattg tcccgcgcat aatcgacaat ggaaggagaa acgccatgag cactgaacac
     1141 tacaatggaa ttttcaggag cctcattcag ttcctgaatg aatttaatac cccttttctt
     1201 catatcagtc acgactctct gattatgaac aatttccttt cttacgtaaa tgtcctcttc
     1261 cggaaacttc tcctgaagat tgtacacata ggatatggcg tatttcaccc cggcacagaa
     1321 tcctctagga ttcgccagat aaattttctc aagagccact aaacacctcc gggatctctg
     1381 ttcttttcag aaaccttcat tataaataca gatatcagaa acaatatctc aagcggaaca
     1441 aacagcatca gcataaatgt cacatcgggc ccgggagaaa acacggctga aaaaatggat
     1501 atgatgattg tagcttctct ccatttttct gccagaaatc ttcctgaaat aattcccagt
     1561 tttccaagca gaatcagcac tacag
//
LOCUS       DAOQGJ010001119         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58412_length_1585_cov_3.209150, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001119 DAOQGJ010000000
VERSION     DAOQGJ010001119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58412_length_1585_cov_3.209150"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(411..1244)
                     /locus_tag="PK683_15615"
     CDS             complement(411..1244)
                     /locus_tag="PK683_15615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH09923.1"
                     /translation="MSVWHFLYLSEFKNYRLPIYRCEKCGMQTIYPKNADYEKMYSED
                     YYSDNNKSDYNYKDERRTERFDAYVWDARIRNIQKYVKSGNFLDIGSSFGGFLKRAAK
                     AGFSVQGVEVSPYSAEYAVKNGIPTVNSDFLNADLPEDHYNVITMVEVIEHLDSPTEV
                     FRKLYSILQKDGLLLIQTADFEGRQAADAERNYHYYLPGHLYCYSRTNLTASLKKAGF
                     KRFILYQGVDFPLRAKLLKSRGSFKSIFDYRRWFNISWYHFKSRLLKGSTSSMVLYAF
                     K"
     gene            complement(1268..>1585)
                     /locus_tag="PK683_15620"
     CDS             complement(1268..>1585)
                     /locus_tag="PK683_15620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH09924.1"
                     /translation="IVSWGVLHYNSTENSKKIIKEYLRMLKPGGLLAGTVRSDRDSFL
                     EVRDNTAETADLKGAFVKLYTQEEVTELLSDFKNLEMGYMERSPVGDVSRRICHWIFK
                     AEK"
BASE COUNT          397 a          349 c          304 g          535 t
ORIGIN      
        1 gacaccgcag gaatctttat ctaacgcagg gtcatacagg ccctgcggct ggggctccaa
       61 aaaatcaagt tcaaagtttt ccattgtctt acctcccgag tctgcaaaag ggatttttta
      121 tccatttctt ttaaatgcct ctggagataa atgttttttt tcctgaatcc gttttgaaat
      181 gatccttttc ttcttctttt ttattcgcgt atgctttttt attaatcgtt ttgtttacct
      241 tttgagtgat ttgtttttga gctgctcttt tggtgtacag tttatttatt gagtgcctgt
      301 tttttggaat atttgggatt gacgttttga gggatcagta tcgaaaaatt ggagagtaaa
      361 atgcggacct gatttcttaa gccgattctt tggaaatgcc ttgtgccttc tcacttaaat
      421 gcatacagca ccatgcttga ggtgctccct ttcagcagtc tgcttttgaa atgataccag
      481 gaaatattga accatcttct gtaatcaaaa atggatttaa agctccctct ggatttgaga
      541 agttttgcac ggagcggaaa atccactccc tggtacagaa taaatctttt gaatcctgct
      601 tttttcagag atgccgtcag atttgtcctg gaatagcagt aaagatgccc cggcaggtaa
      661 taatgatagt tccgctctgc atccgctgcc tgcctgcctt caaaatctgc agtttgaata
      721 agaagaagtc cgtctttctg aaggatgctg taaagctttc tgaacacttc agtcggactg
      781 tccaggtgtt ctattacttc caccattgta attacattgt aatggtcttc cggaagatct
      841 gcattcagga aatcggaatt gactgtaggt atcccgttct tcactgcgta ttcagcagag
      901 tagggagaga cttccactcc ctgaacactg aatcctgctt ttgccgctct tttcagaaac
      961 cctccgaaag aggaacctat gtccaggaaa tttcctgatt tcacatattt ttggatattc
     1021 ctgattctgg catcccagac ataagcatca aatctttcgg tccgcctttc atccttgtaa
     1081 ttataatctg atttattatt atcagagtaa taatcttcag agtacatttt ttcataatct
     1141 gcgtttttgg gatagatggt ctgcatcccg catttttcgc acctgtaaat agggagtctg
     1201 taattcttga attccgataa atacagaaaa tgccagacag acataccgtc ggccgggcac
     1261 tcgagcttta tttctctgct ttgaatatcc aatgacagat cctcctgctg acatcaccga
     1321 ccggactccg ttccatatag cccatttcaa gattcttaaa atcactcagc agttcagtca
     1381 cttcttcctg tgtatacagc ttcacaaagg cacccttcag atcggcagtt tctgcagtat
     1441 tgtcccttac ttcgagaaaa ctgtctctgt ctgaccggac agtaccggcg agaagccctc
     1501 ccggcttgag cattctgaga tattctttaa ttattttttt agaattttct gttgagttat
     1561 aatgaagaac accccagctt acaat
//
LOCUS       DAOQGJ010001120         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58431_length_1585_cov_2.581046, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001120 DAOQGJ010000000
VERSION     DAOQGJ010001120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58431_length_1585_cov_2.581046"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="PK683_15625"
     CDS             <1..1458
                     /locus_tag="PK683_15625"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNH09925.1"
                     /translation="IPAQMNPEKKRKICFMPFRNKGVSPGLKYLQEGIPSVIFSHFHI
                     VHFVFDRNPMPESVIHESGPRQKKKNEEAETFSPETLEEFNADKKIPPPEKDPRYIRL
                     EKEMLREVPPPYSESAQEEGKKRGCFYIVTGEFETAGEDVLKVQAELTERKYGKTERV
                     YGTVSVRRGLQEMEKISSQLKTKLLAEGAVTVKVDAGVPDAFVYLDGEYAGKTPLERN
                     DIHPGRHRLKITKEGFVTLFKLISPAKNRVSSYSFTLMRSEKKARISVSSDPEGASAY
                     LGTQFLGETPVKDAEIPVGPNRLRLMKENYVDHFQGIDAKDGSDLRLNVKMKLGDTEK
                     YYKNRLNVFLDYNYFDFALYSLYSSILFYGTYMYAGYRIDVERDKFYANTNYFNLLFI
                     QSSQGGSASLFSLNQNQQLALFFLQKKTTDSIAASTSYYNNFQTVGTVGAASMLILSG
                     VFYYMGISSDAFEFGFKPDMKRNEASAEMELKFKF"
BASE COUNT          486 a          310 c          354 g          435 t
ORIGIN      
        1 attcctgcac agatgaatcc tgaaaaaaaa aggaaaatct gctttatgcc tttcagaaac
       61 aaaggcgtat cacccggatt gaaatacctg caggaaggaa tcccttccgt aattttttct
      121 catttccata ttgtacattt tgtgttcgac cgaaacccta tgcctgagtc tgtaattcat
      181 gaatccggac ctaggcagaa gaaaaagaat gaggaagctg aaacattttc tccggaaacc
      241 ctcgaggagt tcaatgcaga taaaaaaatc cctcctcctg aaaaggaccc aaggtatatc
      301 cgtctggaaa aagaaatgct cagagaagtt ccgcctcctt actctgaatc tgcacaggag
      361 gaaggaaaaa aaagaggctg tttctacatt gtgaccggtg aatttgagac ggctggagaa
      421 gacgttctga aagttcaggc ggaactgacc gagagaaaat atgggaaaac cgaaagagtt
      481 tacgggactg tttctgtcag aagaggtttg caggaaatgg aaaaaatttc ttctcagctc
      541 aagacaaaac ttttggcgga aggtgctgtt accgtcaaag tggatgcagg tgtgcctgat
      601 gcatttgttt atctggacgg tgaatacgca ggcaagaccc cacttgaaag aaacgatatt
      661 catcctggaa ggcacagact gaagattaca aaggagggat ttgtcactct gttcaaacta
      721 atatctcctg caaagaacag ggtttcctcc tacagtttta cgctgatgag atcggaaaaa
      781 aaagccagga tcagtgtcag ttcagatcct gaaggagctt ctgcttatct gggaacgcag
      841 tttcttggcg agactcctgt aaaggatgca gaaattcctg tcggcccaaa cagacttcgt
      901 ctgatgaagg agaattacgt tgatcatttt cagggaattg acgcaaagga cggctccgat
      961 ctcagactga atgtaaaaat gaagctgggt gatactgaaa aatactataa aaacagatta
     1021 aatgtatttt tagattataa ctattttgat tttgcactct attcccttta cagctccatt
     1081 ctgttttacg gaacctatat gtatgcaggg tacagaatag atgtggagag agacaaattt
     1141 tatgcaaaca cgaattattt caatcttctc tttatccagt ccagtcaggg aggttccgct
     1201 tcccttttct ctctcaatca gaatcagcag ctggcacttt tctttctgca gaaaaaaacg
     1261 actgattcca tcgcagcatc cacatcctat tacaataatt ttcagacagt cggaacagtc
     1321 ggtgcggctt ccatgctgat tctgagcgga gttttttatt atatgggaat cagctccgat
     1381 gcatttgagt ttggatttaa gcctgatatg aaaagaaacg aggcatctgc cgagatggaa
     1441 ttgaaattca aattttaaga atgtttttcc atatatcata ataaaacggc tttactaatt
     1501 tgcgtcgtat ttttttctgg tatcagaaca tgcgggaata gctcagtggt agagcacctc
     1561 cttgccaagg agggggtcgc gggtt
//
LOCUS       DAOQGJ010001121         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58568_length_1583_cov_2.865838, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001121 DAOQGJ010000000
VERSION     DAOQGJ010001121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58568_length_1583_cov_2.865838"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PK683_15630"
     CDS             complement(<1..528)
                     /locus_tag="PK683_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH09926.1"
                     /translation="MRAVVQYFLEKSIFLNLLTALILGVGSYKGFTMNREAFPNINFD
                     IVTITTLFPGASPSEVEKLVTKPIEESVKSVDGIKEFRSGSIENRSGIVITIDPNTKD
                     TQKVVDDIKSAVDRTEDLPEDSKKPLVVELTSGRNPVIEIYVGAKSVNGQSLVTEKEF
                     QEKIKILEQMLLDIPE"
     gene            765..1100
                     /locus_tag="PK683_15635"
     CDS             765..1100
                     /locus_tag="PK683_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH09927.1"
                     /translation="MDIKIEKIGKHTLVKLAGRLDITQSDEVESALTKDIQSGEGDMI
                     INLGQISYISSSGIRVFVGMVRELGRQGRKLKLCNITPPVKKVFDVVELLDLFDVYDN
                     EDQAVASLS"
     gene            1174..>1583
                     /locus_tag="PK683_15640"
     CDS             1174..>1583
                     /locus_tag="PK683_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP phosphate-alpha-4-amino-4-deoxy-L- arabinose
                     arabinosyl transferase"
                     /protein_id="HNH09928.1"
                     /translation="MNPTHFFLFCSVLFILLGTVSGKYFPSVWPDEVLFYSPAKELAD
                     SGMLATKVLEGLIPGMETHTYWMPPAFMGSLALVFKIFHQNIETARLFSLSVSLIGIF
                     IYLQTAKISGLGKKGQILVTALLFSDILFFKVSHT"
BASE COUNT          432 a          345 c          304 g          502 t
ORIGIN      
        1 ctcgggaata tccagaagca tctgttccag tatttttatc ttttcctgaa attccttttc
       61 agtgacaaga gactgaccgt tgacagactt ggctcccaca taaatttcaa tcacagggtt
      121 ccttccggat gtcagttcca ctaccagagg tttcttggaa tcttccggca ggtcttcagt
      181 cctgtccacg gcagacttta tatcatccac caccttctgc gtatcctttg tattcggatc
      241 aattgtaatc acaattccgg acctgttttc gattgagccg gatctgaatt ccttgatacc
      301 gtccacggac ttgacgcttt cttcaatcgg cttagtgaca agcttttcca cctcggaagg
      361 tgaagccccc ggaaacaatg tagtgatagt gacaatatcg aaattgatat tcggaaatgc
      421 ctccctgttc attgtaaatc ccttgtagga acctactccc agaatcagag ctgtcagaag
      481 gtttaaaaaa atgcttttct caagaaaata ttgtacaact gcgcgcatca gcctctccca
      541 aggttcagat tttcttagat accccctatg tcaactttcc tcttcagata attcattaaa
      601 aaccgaacag acaggtctgt cagtcgcact ctttagttat ttgtgctccg gcggcaattc
      661 ctaaacgaac gctgatcacc cttttttttt tttttctatt gacaaaattg gacgggagac
      721 aaaagttcac aacatactcc gagtggaagc aagaggagaa agctttggac attaagatcg
      781 agaaaatcgg caaacatact ttggttaaat tagccggaag attggatatt acacaatctg
      841 atgaagtaga atctgcactg acaaaagata tccagtcagg tgagggcgat atgattatca
      901 accttggaca gatctcttat atttcttctt caggaatcag ggtttttgtc ggaatggtca
      961 gagaattagg ccgtcaggga cgtaaattaa agctctgcaa tattacaccc cctgtcaaaa
     1021 aagtatttga tgtagtagaa cttctggatc ttttcgatgt ctatgacaac gaagaccagg
     1081 cagtagcatc cctctcctga tatgactcta ttactgaatt ttcagttgat tcaaatgatc
     1141 gaaaatttga atttactgta attcagttct gatttgaacc ctactcattt ctttcttttc
     1201 tgctcagtac tgttcatttt actaggaacg gtatcaggga aatattttcc gtccgtctgg
     1261 ccggacgagg ttcttttcta ttccccagca aaagaactgg ctgattcggg gatgcttgcc
     1321 acaaaagttt tggaaggctt gattcctgga atggagactc atacatactg gatgcccccc
     1381 gcttttatgg ggtctttggc tcttgtattt aagatttttc atcagaatat agaaaccgca
     1441 agacttttca gtctctcagt atccttaatt ggaatattta tctatctgca gactgcaaaa
     1501 atctccggtt tgggtaaaaa aggtcagatt ttagtcactg cacttctttt ttcagatata
     1561 ctttttttca aggtttcaca tac
//
LOCUS       DAOQGJ010001122         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58655_length_1582_cov_2.237721, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001122 DAOQGJ010000000
VERSION     DAOQGJ010001122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58655_length_1582_cov_2.237721"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK683_15645"
     CDS             <1..188
                     /locus_tag="PK683_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09929.1"
                     /translation="NKSGTYLGDEVNLNLKWSVFSELQLIFRSGIFRASRAYYAINDF
                     NHGKFAKEAFISAEYKF"
     gene            198..854
                     /locus_tag="PK683_15650"
     CDS             198..854
                     /locus_tag="PK683_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09930.1"
                     /translation="MKNISYAAEINGVKEIILRGKADLSFFQKKLSEYRLVPYSENGS
                     ADITISAVSLEWKKIKFQELTVSVTALTASQEKVFFLPTAFNSNRFLAFSERFFFKTP
                     YYSGNLSSEPENFIIKFSSFEETVLSASKKGGSPVSVENESLNVKVFLPSKNKERYFH
                     AVLQGETETYPFEKEDILEIRPIIEYPVFTWLIESGFKASEWKLRRNAFHAKSRTYSS
                     "
     gene            826..>1582
                     /locus_tag="PK683_15655"
     CDS             826..>1582
                     /locus_tag="PK683_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09931.1"
                     /translation="MQNPEPILPENASVVCIGASNLVRGMPAVAALSRNRFGKNTEIY
                     SVSGHGRSYLGRSSFFFRTLPGILDSGLWEKLSGKKGHIKSIVTDIGNDIMYGYSEDE
                     ILNGAVSAVERLRKLTSDITVTGLPVHNLDSLNEMKFRLFKNIFFPQAEITLADAMNS
                     MKYISESLKNICAQKNLRFVPLEKEWYGFDPIHFRISRWKDAWGKIFSDDPDNRSLAD
                     FSVLEFISLYAKMPEEMELFGKKRNAAQTGIRLK"
BASE COUNT          508 a          299 c          337 g          438 t
ORIGIN      
        1 gaaacaaatc cggcacatat cttggggatg aggtcaatct gaatctgaaa tggagcgtat
       61 tcagcgagct tcagctgatt ttccgttcag ggattttcag ggcctcgaga gcatactatg
      121 ccatcaatga tttcaatcat ggaaaatttg caaaggaagc attcatcagt gcagagtata
      181 aattctagga gaaaataatg aaaaatattt cctatgccgc tgaaataaac ggagtcaaag
      241 agatcattct gaggggaaaa gcagacctgt ctttttttca gaaaaaactt tcagaatacc
      301 gccttgttcc ttattctgaa aacggatcag ccgacatcac aatcagcgca gtttcacttg
      361 aatggaaaaa gatcaaattt caggaactca ctgtttcagt gactgccctc acagcttcac
      421 aggaaaaggt tttttttctg ccgactgcat tcaattcgaa caggtttctt gcattttcag
      481 aaaggttttt tttcaagaca ccttattatt cagggaactt aagttctgaa cctgaaaact
      541 ttatcataaa attttcttct tttgaagaaa cagtcctttc agcatccaaa aagggaggaa
      601 gtcctgtatc cgtagaaaat gaatctctga atgtaaaggt atttcttcct tcaaaaaata
      661 aggaaagata ctttcacgcc gttctgcagg gagagacgga aacgtatccg tttgaaaaag
      721 aagatattct ggaaataaga ccgatcatag aatatccggt ctttacctgg cttatcgagt
      781 caggcttcaa ggcatctgaa tggaaactta ggaggaatgc atttcatgca aaatccagaa
      841 cctattcttc ctgaaaatgc atctgtagtc tgcattggtg cgagcaatct ggtaagagga
      901 atgcctgcag tcgcagccct ttccagaaac aggttcggaa aaaatacgga aatctattct
      961 gtttcaggac acgggagatc ttatctgggg aggagcagtt tctttttcag aactcttcca
     1021 ggaatacttg attcaggcct ttgggaaaag ctttccggaa aaaaagggca tattaaatcc
     1081 attgtcacgg atatcggaaa cgacattatg tacggttact ctgaagatga aattctgaac
     1141 ggagcagtca gtgcggtgga aagactgaga aaactcactt ccgacattac agtgacagga
     1201 ctgccggttc acaatctgga ttcactgaat gaaatgaaat tcagactgtt taaaaatatc
     1261 ttctttcccc aagccgaaat aaccctcgct gatgcaatga attcaatgaa atatatttca
     1321 gaatcattaa aaaatatatg cgcgcagaaa aatctccgtt ttgttcctct ggaaaaagaa
     1381 tggtacggat ttgacccgat tcattttagg atcagcagat ggaaagatgc ctgggggaaa
     1441 attttttcgg atgatccgga caatcgatct cttgcagatt tttcagtttt ggagttcatt
     1501 tcactctatg caaagatgcc ggaggaaatg gaacttttcg gaaagaaaag aaatgcggcg
     1561 cagactggaa ttcggctgaa aa
//
LOCUS       DAOQGJ010001123         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58736_length_1580_cov_3.916721, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001123 DAOQGJ010000000
VERSION     DAOQGJ010001123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58736_length_1580_cov_3.916721"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK683_15660"
     CDS             <1..234
                     /locus_tag="PK683_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH09932.1"
                     /translation="NIYLGGDKSVHYNTMKMTLPEAINHVRAKIISNPEMKVLIHADR
                     NIPFSEVDAVFEKLKEIGATKISLVTQTNPGGG"
     gene            236..889
                     /locus_tag="PK683_15665"
     CDS             236..889
                     /locus_tag="PK683_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNH09933.1"
                     /translation="MSIDLKPKEEEKEEKIFFDARVPKLHEKTFKENTYRFIDRNRFN
                     LGISISATLQILMILFWQTPKLDFSKAEKPLDDVAFVDNVSIKDPNANDSVANDGEIE
                     LTDKKKTDEKEDPRISGAQDLDFAGATPPVDLNPGIEPEYTAEARENGITGIVTLQIV
                     IADSGEVLQVRAVGKPIGGGLEESAVRAYRKKRFAPSILDGKPLTVRVNIPVRFRIQ"
     gene            complement(1007..>1580)
                     /locus_tag="PK683_15670"
     CDS             complement(1007..>1580)
                     /locus_tag="PK683_15670"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH09934.1"
                     /translation="EKKPFYTEQEYLEIDRNSELRNELLDGEIYAMTGASLKHNIISL
                     NIASSLKSLFKQKECKVFMADLRVNVSKGLYTYPDILIVCGNILFTDDHLDTVKNPKC
                     IIEVLSDSTEKYDRGEKFRNYRKIESLEEYILISQNEILAEKYYRKKEDFWEYSVFDG
                     LDKSVNIDSIGIRLSMQEIYAETDLMTASP"
BASE COUNT          496 a          312 c          328 g          444 t
ORIGIN      
        1 aacatctatc tcggaggaga caaatctgtt cattataata caatgaaaat gactctgccg
       61 gaagcgatca accatgtcag agcaaagatt atctccaacc ccgaaatgaa agttctgatc
      121 catgcagaca gaaacatccc attttcagaa gtggatgctg tatttgagaa actgaaggaa
      181 atcggtgcaa cgaaaatatc attagtaaca cagaccaacc ctggaggagg ctgatatgtc
      241 cattgacttg aaaccaaagg aagaagagaa agaagaaaaa atattttttg atgcaagagt
      301 acctaaattg catgaaaaaa catttaaaga aaatacatat cgttttatag acagaaacag
      361 gtttaatctg gggatctcga tttcagccac tctgcagata ctgatgattc ttttctggca
      421 gaccccgaag ctggacttca gcaaagcaga aaaacctttg gatgatgtag catttgtgga
      481 caatgtttcc atcaaggatc cgaatgcaaa tgattctgtg gcaaatgacg gtgagataga
      541 actgaccgac aaaaagaaga cagacgaaaa agaagatccc agaatctccg gagcacagga
      601 tctggacttt gcaggtgcca ctccgccagt tgacctgaat ccgggaatag agcctgagta
      661 cactgccgaa gccagagaga acggcatcac tggaattgta actctgcaga ttgtcattgc
      721 agacagcggg gaagttcttc aagtgagagc tgttggaaaa ccgattggcg ggggacttga
      781 agaatctgcg gtcagagctt acagaaagaa aagatttgcg ccttccattc tggacgggaa
      841 accgctgaca gtgagagtga atattcctgt aagattcaga atccaataat tgtttttcat
      901 ctaagcttct gttcgctctt taaattggag cctgcaggag ttttcagttt tttaatccat
      961 aaaagactga tgggatgagg cagaacagaa tcaaagctga caatgcctat ggacttgcag
     1021 tcatcagatc agtctctgca taaatttcct gcattgagag tctgatccca atggaatcga
     1081 tatttacaga tttgtccaat ccgtcaaaga cactgtattc ccaaaaatct tcttttttcc
     1141 gatagtattt ttctgcaaga atctcattct gcgatataag aatgtattct tcgagagact
     1201 caatctttct ataattccgg aacttttctc ccctgtcgta tttttctgtg gaatccgaga
     1261 gcacttcaat tatgcattta ggatttttca ctgtgtccag atgatcatca gtaaatagaa
     1321 tatttccgca tacgatcaga atatccggat atgtatacag tcctttggaa acattgaccc
     1381 tcaggtcagc cataaagacc ttgcattctt tctgcttaaa cagacttttt aagctggaag
     1441 ctatatttaa tgaaatgata ttgtgtttta gcgaagcgcc tgtcattgca taaatttctc
     1501 cgtccaagag ttcgtttctg agttcagaat ttctgtctat ttccagatac tcctgctcag
     1561 tatagaaagg tttcttttct
//
LOCUS       DAOQGJ010001124         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58827_length_1579_cov_2.582021, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001124 DAOQGJ010000000
VERSION     DAOQGJ010001124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58827_length_1579_cov_2.582021"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..225)
                     /locus_tag="PK683_15675"
     CDS             complement(58..225)
                     /locus_tag="PK683_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09935.1"
                     /translation="MEPNRCSNKNVIGVCIQKSTVIAAEWVYYSGDFSLAEAKAKCDE
                     LKTQLTAGEQR"
     gene            929..>1579
                     /locus_tag="PK683_15680"
     CDS             929..>1579
                     /locus_tag="PK683_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09936.1"
                     /translation="MVTVHMQIRIEEANKKLKTGEFEKAKKIFSELLDISPENLELIS
                     GYYIASYWDNRLDKILKTPEGIERGLFLNESFKRFEKDLSERKYPRTLSFESILICVL
                     QESCSQLRQGYQRDGKYVMTKEIMIILTANLIRIRDYKNCSEMIDYLRKNQDIPREFY
                     FYKAECLYHTGEHRKSRMLYRSSLLYYQELLPLDLVESEPLSAAIYDLKKNFNDDDL"
BASE COUNT          496 a          287 c          302 g          494 t
ORIGIN      
        1 aaacatttta cttaagcggt aaatagacag tattcgggtc atactcagta aggaatttta
       61 cctctgttcc cctgccgtca actgtgtctt cagctcatca catttcgctt tggcttctgc
      121 gagagaaaaa tcgccggaat aatacaccca ctcagctgca ataacagtgc ttttttgaat
      181 gcatactcct atgacgtttt tgtttgaaca ccggttaggt tccaatcttt caccttctgt
      241 ctgttgaaca gaaggttttt taaagctgtt ttgacagctg aaataaggag tttttgtatt
      301 ccaagaacct ctaaagggaa tcggagtcag agttgcatca attaaaagta cagttgctgc
      361 attttccgaa ccagacattg ttttgctttt cttcccttgt ccaaaatctt tgcagttgaa
      421 tagtccgagt atgaccaata gaattacttt tttcatggac atgacccacc gtaataaagt
      481 agaacatcag aattcgtttt gatcaacgtt gaaatgcccg ccaatgcaag tttcctctca
      541 gtgtccgatt ttccgtcatt tatattgcat ccgataaaaa tagatgcaaa aataaaaaat
      601 ttaacctttt taattttcat tttgcttatc cattttttta gagtcgttat tgactcaaat
      661 ttacttaagg acagattttt ttatctggtt tccctgtaaa gaagtaaata ttaggagaat
      721 ttcgatttat taatatttaa agtaagtttt ttcaatgtca taacttgatt cgacttactg
      781 agatactctt cagcgatata atgaggtaac ttgggacgga tggaggcagc ttaaaaatga
      841 acctgatccg aaaatcaagc tcctgtttga agcttaaaaa cattcagcaa aaatgaaatt
      901 aagtgacatt cagtgaaaaa aatggagatt ggttactgta catatgcaga tcaggattga
      961 ggaggcaaat aaaaagctga aaacaggaga atttgagaaa gcaaaaaaga ttttttctga
     1021 acttctcgat atcagccccg aaaaccttga actgatttcc ggttattata tagcatccta
     1081 ctgggacaac cgtctggata agattttaaa gactccggaa ggaattgaaa gaggtctgtt
     1141 tttgaacgag agtttcaaga gattcgaaaa agatctttct gagcggaaat atccaagaac
     1201 cctttccttt gaatccatcc taatctgtgt tctgcaggaa tcctgttcac agctcaggca
     1261 gggatatcag cgggacggaa agtatgtgat gacgaaagaa attatgatta tcctgactgc
     1321 caatctgatc cgcatacggg attataaaaa ctgcagtgag atgattgatt atcttcgaaa
     1381 gaatcaggac ataccccgtg agttttattt ttacaaggca gagtgtctat atcatactgg
     1441 agaacataga aaaagcagaa tgctgtacag aagcagtctg ctctattatc aggaacttct
     1501 tcctctcgat ctggtggaat ctgagcctct ctctgctgcg atttacgatc tgaaaaaaaa
     1561 ttttaacgat gatgatctg
//
LOCUS       DAOQGJ010001125         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58832_length_1579_cov_2.462598, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001125 DAOQGJ010000000
VERSION     DAOQGJ010001125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58832_length_1579_cov_2.462598"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..712)
                     /locus_tag="PK683_15685"
     CDS             complement(137..712)
                     /locus_tag="PK683_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09937.1"
                     /translation="MNSKLSGASLHFNEIIITIDGKRTQWKHFSQGKFKIWGKPAGYN
                     INFESGNVNCPGCTQKTERPVGPFSEYFKLYPLAVLWTIGFQIPFTVFDLLSLPLRIL
                     PQEEIHSGLKQKLRTEQIEKLRISYENNAGGDRTFIVLEKGKEITVPIERIFIPNTVF
                     SLNFYDENGLFTQSRWIEIPEFVKAIRITLK"
     gene            complement(1153..>1579)
                     /locus_tag="PK683_15690"
     CDS             complement(1153..>1579)
                     /locus_tag="PK683_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09938.1"
                     /translation="HRRLYDLSLDGRKSKRRRASFSVLNRTAEKREQRSSCKKCKKKH
                     IVKKIIKNAECSFDNPVMEALYSLLLNEETEKYHIFGAHKKGSAVETDKDSFCGKAVA
                     KDLKSVKAGVDEKALDKALDDLDDDEICGNCLKSYEAED"
BASE COUNT          449 a          323 c          305 g          502 t
ORIGIN      
        1 gcacaaacac gaaaaaatct atcttcatgc tcagacggaa gtcaagagat tttatgagaa
       61 acatggattc gtttcggaag gagaggaatt tattgaggcg gatattcctc acataaaaat
      121 gatattgatt aattaattat tttaacgtaa ttcgtattgc ctttacaaac tcagggattt
      181 caatccatct cgactgtgtg aacagaccgt tttcatcata aaaattcaga ctgaatacgg
      241 tgttcggaat gaatattctc tcaataggaa ctgtgatttc cttccctttt tccagaacaa
      301 taaatgtcct gtctccgcct gcattgtttt cataggaaat cctaagtttt tcaatctgct
      361 ctgttctgag tttctgtttc agccccgaat gaatttcttc ctgtgggaga attcgaagcg
      421 gaagactcag aagatcaaag acagtgaagg gaatctgaaa tccgattgtc catagaactg
      481 caagaggata aagcttgaaa tactcactga atggtccaac cggcctttct gtcttctgag
      541 tgcatccggg acaatttaca ttccctgatt caaaattaat attatagcct gcaggttttc
      601 cccagatttt gaattttccc tggctgaaat gtttccattg agttctcttt ccgtctattg
      661 taatgattat ttcattaaaa tgaagtgatg cccccgagag ttttgaattc aaatctatct
      721 catagctcaa ataagattcg gttttcggat ccgccggaac tgcactcgaa agattccttt
      781 ctacctgaat ccgcagacag tgactgaata aaataagcag aatcaggctg agtttcgaaa
      841 atagcttcac tgagtttaga ctgttttttt aggggctata agccgtgtga gcactttaaa
      901 gaaagttttt tggattttaa ggattcctgt ttttggaagg gaactgctgc gattcggaaa
      961 ctccagtccg gaaagagaac ggttgaaatg cagaaactcc agcgtgggaa ataataggtc
     1021 cagctttaaa aaagaactgc gatgacataa aacagaagct gtgtaagaga aacctttatt
     1081 gaacgggact ttacagaaaa aactgacgga tcaaagttcc attttgttct ttacacagcc
     1141 ttaatcgaca gatcagtctt cagcttcgta agacttaaga cagttcccgc agatttcatc
     1201 atcgtccaga tcatcaagag ctttatcaag tgctttctca tccactccgg ccttgacact
     1261 tttcagatct ttcgcgactg cttttccgca gaaggagtct ttatctgttt ctaccgcaga
     1321 gcctttttta tgtgctccga aaatgtgata cttttcagtt tcttcgttga gtaatagaga
     1381 gtatagggct tccataaccg gattatcgaa agaacattca gcattcttaa taattttctt
     1441 gactatatgt ttttttttgc attttttaca gctgcttctt tgctcccgtt tctctgccgt
     1501 ccggttcagt actgagaaag aagcccgccg tctcttgctc ttccttccat ccagagaaag
     1561 atcatataac ctgcgatgc
//
LOCUS       DAOQGJ010001126         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_58874_length_1578_cov_3.605384, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001126 DAOQGJ010000000
VERSION     DAOQGJ010001126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58874_length_1578_cov_3.605384"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PK683_15695"
     CDS             complement(<1..371)
                     /locus_tag="PK683_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09939.1"
                     /translation="MISKIFLFPAAFLLIGCSLKAQFTFTESQSAAEKQLIGEEKEIE
                     KDGWLLSSLKTSATGPEEWKSVSDLEFSDREKAKEYIELIKTVYYTAPEIRKLKLLHF
                     AGESLSGKLQILDFTKKDDFN"
     gene            complement(361..702)
                     /locus_tag="PK683_15700"
     CDS             complement(361..702)
                     /locus_tag="PK683_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH09940.1"
                     /translation="MKIKVTTKNEVHLVKIEGPIKAGNEFELGEKIEQYIKKGVVPKF
                     IIDLKKVPFINSAGLGIFLNIYKHIDGLNGRMVFANLSSDVENLMEITKLSTVFEIFR
                     NADEAMESFDF"
     gene            complement(744..992)
                     /locus_tag="PK683_15705"
     CDS             complement(744..992)
                     /locus_tag="PK683_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HNH09941.1"
                     /translation="MEIIGYIGAALTTAAFLPQALRVIQTKQTRDISRNMYILLTAGI
                     CFWLVYGFMKNSVPILLANSVTLIFSVMILFYKLKEEK"
     gene            complement(1072..>1578)
                     /locus_tag="PK683_15710"
     CDS             complement(1072..>1578)
                     /locus_tag="PK683_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09942.1"
                     /translation="EILLDKNSILLPEYGMNSESEKGLHSLWKEIQEIAGEGTLVLDA
                     GSGLTYLSALRHLENDRLSLCAVSLGEKKEKMEKRLSLYWKDFFPDENEPYFRLIRPV
                     TGASFGSVNRELQNFIQNESKKGFYLEPVYSAKTVYTILKLAEQGSLSGNIWYLHQGG
                     RLNHWRLF"
BASE COUNT          438 a          333 c          291 g          516 t
ORIGIN      
        1 ttgttgaagt catctttttt tgtaaaatcc aggatctgca gtttccctga cagtgattcc
       61 cctgcaaaat gaaggagttt cagctttctg atttccggag cagtgtagta gactgttttt
      121 atcagttcaa tgtattcctt cgccttttcc ctgtcggaaa actcgagatc agatacactt
      181 ttccattctt caggtcctgt tgcagatgtt tttagggaac ttagaagcca gccgtccttt
      241 tcaatttcct tttcttctcc aatcagctgc ttttcagccg ccgactgtga ttctgtaaat
      301 gtaaactgtg ctttcagaga acagccgatc aaaagaaatg cggccggaaa aagaaaaatt
      361 ttagaaatca aatgactcca tagcttcatc ggcatttctg aagatttcaa atactgtaga
      421 gagttttgta atttccatca ggttttccac gtctgaactc aggttggcaa aaaccatcct
      481 gccgttcaat ccatctatat gtttgtaaat atttaaaaaa atacccagcc cggctgaatt
      541 gatgaaagga actttcttca gatcgatgat aaatttagga actacccctt tcttaatata
      601 ctgttcaatt ttttcaccaa gttcaaattc atttcctgct ttgattggac cttctatttt
      661 aaccagatga acttcgtttt tcgtagtgac cttaattttc ataaacttag gtctactatt
      721 tttaaaaaac gaatacggta aagttatttt tcttctttaa gcttgtaaaa aagaatcatt
      781 acgctgaaaa ttaatgtgac tgagtttgcg agaagaatag gaactgagtt tttcataaac
      841 ccgtaaacca gccagaaaca gatgcccgca gtgagaagga tgtacatatt tcgtgaaatg
      901 tctctcgtct gctttgtctg aatgactctc agtgcctgcg gaaggaatgc tgcagtagtc
      961 agtgccgctc cgatatatcc tatgatttcc ataaatgcct ctgaaaatat tgaatgtcaa
     1021 tggttttttt cctaagtttt tagcaaagca gtaaatcaga ggactcagag gtcagaaaag
     1081 ccgccagtga ttcagtcttc ctccctgatg cagataccag atatttcctg aaagagagcc
     1141 ctgttccgcc agtttaagaa ttgtataaac agtctttgcg gagtagacag gctccagata
     1201 gaatcctttc tttgattcat tctggatgaa attctgcagt tccctgttca ctgatccgaa
     1261 ggaagcgcct gtcaccggtc tgataagacg aaaatacggt tcattttcat ccggaaaaaa
     1321 atctttccag taaagactca gtcttttttc cattttttct tttttttcac ccagactgac
     1381 tgcacacagg gaaagcctgt cattttcaag atgcctcagg gcactcagat atgtgaggcc
     1441 tgatcctgca tcaagaacca aggttccttc tcctgcgatt tcctgaattt ccttccataa
     1501 tgaatgcagt cctttttccg attcagagtt cattccgtat tccggcagaa gaatcgagtt
     1561 tttatcgagt aagatttc
//
LOCUS       DAOQGJ010001127         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59125_length_1574_cov_3.111257, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001127 DAOQGJ010000000
VERSION     DAOQGJ010001127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59125_length_1574_cov_3.111257"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK683_15715"
     CDS             complement(<1..214)
                     /locus_tag="PK683_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09943.1"
                     /translation="MNELFYIACAYIAIMLFKTVFSAVYAAQFRKKYPSPDSKKTVTV
                     LQAVMGGDPELKTVLEKNIKENRGIAF"
     gene            complement(211..1161)
                     /locus_tag="PK683_15720"
     CDS             complement(211..1161)
                     /locus_tag="PK683_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH09944.1"
                     /translation="MKIMITGASGFVGSSFMKKYSAKNDVEILGTGRRKSDLKNYIQN
                     DLTKPLETDFRPDTVIHCAAKASPWGSREEFERQNVLATENVIDFCHRNNVKKLIYIS
                     SSSVYYNTEDQIGLTENSPIGPKFINEYARTKYLGELAVKKFERDWVILRPRAVFGPG
                     DTVLFPRILEAAKKRTLPVISREKSPAVGDLIYIDNLTECMYLSCIRNDIRGEFNLTN
                     NEPVEIQSFLFSIFRSLGLPVPSFRISYETAIAGAGFLEGIYNFFHLNSEPPVTQFGI
                     HVFALSKTFNVSKMLKTFGEPKVKISEGIERFIEWQRKQK"
     gene            complement(1158..>1574)
                     /locus_tag="PK683_15725"
     CDS             complement(1158..>1574)
                     /locus_tag="PK683_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09945.1"
                     /translation="TRKTQGFIRFRLNDILKIKKEPCPCGNPAERIRSIEGRSDEILY
                     FTELETRELYPVFPDTVRQIMYTVSEKLQDYRIIQKADTLIFFLKTENEEDISSKIIS
                     AFSALSLDMNLRKPAFRFEKWFPQPPEEKKKRIRRI"
BASE COUNT          403 a          337 c          312 g          522 t
ORIGIN      
        1 tgaacgctat tcctctgttc tccttaatgt tcttttccag tacagttttc agttcaggat
       61 cgccgcccat caccgcctga agtactgtca ctgttttttt tgaatcagga ctcggatatt
      121 tttttctgaa ctgcgcagca tagacagctg agaaaactgt cttgaaaagc attatggcaa
      181 tataagcaca cgcaatataa aataattcgt tcatttctgt ttcctctgcc attcaatgaa
      241 tctttctatc ccttcagaaa ttttcacttt cggttcaccg aatgttttca gcattttgga
      301 gacattgaat gtcttggaaa gagcaaaaac atgaatcccg aactgagtga ctggaggttc
      361 tgaatttaga tgaaagaaat tataaatgcc ttcgaggaat cctgctcccg caattgcagt
      421 ttcataggag atgcggaagg aaggaaccgg cagtccgaga cttctgaata tgctgaaaag
      481 aaaagactga atttccacag gttcgttgtt agtaagattg aattctccgc gtatatcatt
      541 acgaatacat gataaataca tacattccgt aagattgtct atatagatca gatcgcctac
      601 tgcaggtgat ttttctctgc tgatgacagg cagggttctt tttttggcag cttccaggat
      661 tctaggaaaa agcactgtgt ctcccggacc gaaaacggct cttggacgga gaatcaccca
      721 gtccctttcg aattttttta cagccagctc cccgaggtat tttgtccttg catactcgtt
      781 gatgaatttc ggaccgatag gtgaattctc agtcagtcca atctggtcct cagtattgta
      841 gtaaaccgag ctggaagata tataaatcag tttttttaca ttatttctgt gacagaagtc
      901 aattacgttt tcagttgcaa gcacattctg tctttcaaat tcttctctgc ttccccaggg
      961 agaggctttg gctgcacagt gaatcactgt atccggtctg aagtctgttt ccagaggttt
     1021 cgtaaggtca ttctgaatat agtttttcag atctgatttt cttcgccctg tccctaggat
     1081 ctcaacatca ttcttggcag aatatttttt catgaaggaa cttcctacaa accctgaagc
     1141 tcctgtgatc attattttca tatacggcgt atcctctttt tcttttcttc aggcggctgc
     1201 ggaaaccatt tctcaaaccg gaatgcaggt tttctcaggt tcatatccaa tgacagtgca
     1261 gagaatgcag agataatttt gctcgaaatg tcttcttcat tttccgtttt cagaaaaaaa
     1321 atcagtgtgt ccgctttctg gatgattctg tagtcctgga gtttttctga gactgtatac
     1381 ataatctgtc tcaccgtatc cggaaaaaca ggatacagtt ctcttgtttc cagttccgta
     1441 aaataaagta tctcatccga cctgccttct atggaccgga ttcgttctgc agggtttccg
     1501 caggggcatg gttctttttt aattttcaga atatcgttca gtctgaaccg aatgaacccc
     1561 tgtgtttttc tggt
//
LOCUS       DAOQGJ010001128         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59150_length_1574_cov_2.502304, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001128 DAOQGJ010000000
VERSION     DAOQGJ010001128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59150_length_1574_cov_2.502304"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PK683_15730"
     CDS             <1..599
                     /locus_tag="PK683_15730"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HNH09946.1"
                     /translation="LEQMKNSINQSAAALSQFQGTYTSVLNEFNQLNKELLDLLDIQK
                     IITNDTENNARMLFRSTYEINEIRKKNLEQIASPTLLLMIRTVKTEHMLWKWWLISYL
                     FGLYHIPEEQVLDHTQCRLGKWYYSVQNPAIKDLSCFRDMEEPHSELHRLAKSIFQQI
                     KNNDVIRARSEINELENVSFKIIGFLDSLEKEVIRKGL"
     gene            679..1119
                     /locus_tag="PK683_15735"
     CDS             679..1119
                     /locus_tag="PK683_15735"
                     /inference="COORDINATES: protein motif:HMM:NF039314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5991 domain-containing protein"
                     /protein_id="HNH09947.1"
                     /translation="MKKYIILFLMLVPAVLFAKEADWSGKYNGSKDEGTNAGGTPIFY
                     AYAIHIKKTGRKYSVEYSEDGYQTMTRIQGELIPEKEGFAKIIFLGWGEENSTSYQNY
                     KKGDVIMRIKRKKNGVCEVRYQNDEFKKEPVQFIQEKETSPSKK"
     gene            1394..>1574
                     /locus_tag="PK683_15740"
     CDS             1394..>1574
                     /locus_tag="PK683_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH09948.1"
                     /translation="MIYSQCLKFAETKEEAEDMAQEIFLKAFESLSKFRGESMFSTWL
                     FQIGRNLVLEKIRKKN"
BASE COUNT          558 a          280 c          318 g          418 t
ORIGIN      
        1 ctcttgagca gatgaaaaac tccatcaatc agagcgcagc tgcactttct cagtttcagg
       61 gaacatatac atctgtcttg aatgaattca atcagctgaa caaagagctt ctggatctcc
      121 tggatataca aaaaatcatt acaaatgata cagaaaataa tgcaagaatg cttttcagat
      181 ccacatacga gataaatgaa atcagaaaaa aaaacctgga gcagattgct tcgcctaccc
      241 ttcttcttat gataaggaca gtaaaaacag aacatatgct ctggaaatgg tggctgatct
      301 cctatctttt cggtctctac catattcctg aagaacaggt gcttgaccat acccagtgca
      361 gacttggaaa atggtactat tctgtacaga atcctgcaat caaggatctt tcctgtttca
      421 gagatatgga agaacctcat tcagaacttc acagacttgc aaagtccatt tttcagcaga
      481 tcaaaaacaa cgatgtgatt cgcgccagat cggaaatcaa tgaactggaa aatgtatcct
      541 tcaaaatcat aggatttctg gacagcctgg aaaaagaagt catcaggaaa ggtctgtaaa
      601 aacgtttcag tttaaaaaga attcagtatc tgccaaaaaa aggttgaagg ccttttctgt
      661 ctgaaaatta tttactccat gaaaaaatat attattttat ttcttatgct tgtgccggct
      721 gttctttttg caaaagaagc agactggtca ggaaaataca acggttcaaa agatgagggc
      781 accaatgcag ggggaactcc cattttttat gcatacgcaa tccatataaa aaaaacagga
      841 agaaaatatt ctgttgaata ctctgaagac ggctatcaga caatgacaag aattcaggga
      901 gaactgattc ctgaaaagga aggatttgca aaaatcatct tccttggctg gggagaagag
      961 aattccactt cctatcagaa ctacaagaaa ggcgatgtca ttatgaggat aaaaagaaag
     1021 aaaaacggtg tctgtgaagt cagatatcaa aatgacgaat tcaaaaaaga acccgtccaa
     1081 tttattcagg aaaaagaaac atctccctct aaaaaataat ctgctgaagt ttttgcgaaa
     1141 tcgtcagata ttcttttctt gacaggagca taggcagaca gatcctagac ctatcatagt
     1201 ttggaattat ttctgactgt ggcagaggag ggttaggttt attttatttg aaactgagtc
     1261 cgttaagctg ctgcgaaatg taagggaagc tgaagcggaa attaaatgca atccggagga
     1321 ctggaaaatt atccaggaaa ttctttccgg aagaaacaaa aagtttgagc ttctgctcga
     1381 aaaatatgag ggaatgattt attcccaatg tttaaaattt gcagaaacca aggaagaagc
     1441 ggaggatatg gctcaggaaa ttttcctgaa agcatttgaa tctctctcaa agttcagagg
     1501 cgagtctatg ttctccacat ggctttttca gattggaaga aatctggttc tggaaaaaat
     1561 cagaaagaag aatc
//
LOCUS       DAOQGJ010001129         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59198_length_1573_cov_2.895916, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001129 DAOQGJ010000000
VERSION     DAOQGJ010001129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59198_length_1573_cov_2.895916"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1573
                     /locus_tag="PK683_15745"
     CDS             <1..>1573
                     /locus_tag="PK683_15745"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNH09949.1"
                     /translation="RLGGHLSLSDRIQKPSEKFLQKIQEYAALLGNEGTDCLERNPGE
                     PWRQYVSLVYKKLPMNSERLNYHYSASEELLEDLEFLKESLLSINAVRIVEKEIIPAE
                     RVIQSFGFHLAVLDIRQNSKYHDRALSQLLKAAGIFDYDFSVWSEEKRISFLTQELNS
                     PRPFVLPDMQCGTEADSVVGCLRVIARHIKKYGYNSLGALIVSMTRSLSDLLTVFILA
                     REAGLLIPTEEGLVCPLPVVPLFETIEDLERAPDILERFLNHPMTRRSLRYTAKIKKY
                     PVPIQQVMLGYSDSNKDGGILASQWNLYTAQKKLTETGNRNTVKIQFFHGRGGTISRG
                     GGKTHRFMQVLPHTSFTGSIRMTIQGESIAQQFANWNNATYNLELLIASAASTAIKHR
                     SLSKKEHPLEEYMESLSVRSKEVYQELLKSPGFMEFYGQATPIDIIENSRHGSRPSRR
                     TGKRTLGDLRAIPWVFSWNQSRFILPGWYGAGTALSELKVKNPGVFELLPSEVRSWHF
                     FRHAVMNIEANLMSVD"
BASE COUNT          468 a          327 c          382 g          396 t
ORIGIN      
        1 taggctgggc gggcatttaa gtctttctga cagaattcag aagccttctg aaaaatttct
       61 gcagaaaatt caggaatatg cagccttgct gggaaatgaa ggaaccgact gtctggaaag
      121 aaatccggga gagccttgga ggcagtatgt cagtctggta tataaaaaac tcccaatgaa
      181 ttcagaaaga ttaaattatc attatagtgc ttctgaggaa cttctggaag atctggaatt
      241 tctgaaagaa agtcttctga gcataaacgc agtcagaatc gtggaaaagg aaatcattcc
      301 tgcagaaaga gtcattcagt ctttcggttt tcatctggcc gtactggata tccgtcagaa
      361 cagcaaatac catgacaggg cgctgtctca gcttctgaaa gctgccggaa tttttgatta
      421 tgatttttct gtctggagcg aagaaaagag aatctccttt ctgacgcagg aactgaattc
      481 cccgcgtcct tttgttcttc cggatatgca gtgcgggacg gaggcggatt cagtcgtcgg
      541 ctgtctgaga gttatagcaa gacatatcaa gaagtacgga tacaattcat taggtgcact
      601 gatagtaagt atgactagaa gtctttccga tcttctgaca gtcttcatcc ttgcgagaga
      661 ggctggactt ctgattccga ccgaagaggg acttgtctgt ccgcttcctg ttgtgccgct
      721 ctttgagaca attgaagatc tggaaagagc gcctgacatt ctggaaagat ttttgaacca
      781 tccgatgacc agaaggagtc tgaggtatac cgcaaaaatt aaaaaatatc ccgtaccgat
      841 tcagcaggta atgctcgggt acagcgacag caacaaggat ggaggaattc ttgccagtca
      901 atggaatctt tatactgcac agaaaaaact gactgagacg ggaaaccgca atacagtaaa
      961 aattcagttc tttcacggac ggggaggaac gatcagcagg ggaggaggaa aaactcacag
     1021 gtttatgcag gtgcttcctc acacttcatt taccggaagc ataagaatga caattcaggg
     1081 agagtccatt gcacagcagt tcgcaaactg gaacaatgca acctacaatc tggagctgct
     1141 tattgcaagc gctgcatcca ctgcaatcaa gcatagaagt ctttccaaaa aggaacatcc
     1201 gctggaagag tatatggaat cattatcagt aagaagcaag gaggtctacc aggaacttct
     1261 gaaatctccc ggatttatgg aattctacgg acaggcgacc ccgattgata tcatagaaaa
     1321 cagccgtcac ggttccagac cctccagaag aaccggaaag aggacactgg gagatcttcg
     1381 tgctattccc tgggtattca gctggaacca gtccaggttt attcttccag gatggtacgg
     1441 agcaggtact gctctttctg aactcaaagt taaaaatccc ggagtcttcg aactgcttcc
     1501 ttccgaggtc agaagctggc attttttccg tcatgcagta atgaatattg aggcaaattt
     1561 aatgagcgtt gat
//
LOCUS       DAOQGJ010001130         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59199_length_1573_cov_2.801054, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001130 DAOQGJ010000000
VERSION     DAOQGJ010001130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59199_length_1573_cov_2.801054"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PK683_15750"
     CDS             complement(<1..1234)
                     /locus_tag="PK683_15750"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HNH09950.1"
                     /translation="MKTSKYTFLAKKYRSGDIMAVNYLSGSIDLIEKEEASEFQQRLA
                     SGSWEDYHLRDYLLERGYIYRNEDEEEEKIREKYLEFSDEYEKTPVQLIFSTSFVCNF
                     ACKYCFQEEYQNSKKMLDQEITDSFFSHINSRFADEKVRPYITVFGGEPLLGAENYRK
                     SILYFLENSVKYNYPVAIVTNGYELSNYIPEFVKRKINIREIQVTVDGDKEHHNERRI
                     LKGGKSTFDRIMDGIDDALRAGYRINLRSIIDRENIESLPKLAEYCQERGFLDYPSSQ
                     FETTIGRNYELHTCQKTDKLFDRFNIWENFFELSEKYPILKKYHRPQFHGMRYLAENG
                     SLPFPVFDGCPAGKKEWAFDIHGYIYGCTASVGVEKYRLGSYLDPSFPADEKQLLEWK
                     TRDVLTVPECRDCAVSLSC"
     gene            complement(1389..>1573)
                     /locus_tag="PK683_15755"
     CDS             complement(1389..>1573)
                     /locus_tag="PK683_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09951.1"
                     /translation="GAVPKQEYFAAVASGGFKEAEILEESEPYEKGGVIVRSITVRGH
                     KISAQKKTCGCGGGNC"
BASE COUNT          401 a          379 c          287 g          506 t
ORIGIN      
        1 cgcaggaaag actgacagca caatccctgc attcaggaac tgtcagcaca tcccttgtct
       61 tccattccag aagctgtttt tcatctgcag gaaaagacgg atccagataa cttcccagcc
      121 tgtatttttc cactccgaca gaggcagtgc atccgtaaat atatccgtga atatcaaagg
      181 cccattcctt tttgcctgca ggacacccgt caaacaccgg gaaaggaagg cttccgtttt
      241 cagccagata tctcatcccg tgaaactgag gcctgtgata ttttttcagt atgggatatt
      301 tttctgaaag ttcaaaaaaa ttctcccaga tattaaatct gtcgaagagc ttgtcagtct
      361 tctggcaggt atgcagttca taattccttc cgatggttgt ttcaaactga gaggacggat
      421 aatcgagaaa tcctctttcc tgacagtatt ccgcgagttt aggaagagac tcaatatttt
      481 ccctgtcgat aattgatcgt agattaattc tgtatcctgc acgcagagca tcatcgatcc
      541 cgtccatgat tctgtcaaaa gtggacttgc cgcccttcag aatccttctt tcattgtgat
      601 gctctttatc tccgtccact gtcacctgga tttcgcgtat attgattttt ctttttacga
      661 attcaggaat gtaattcgaa agttcgtacc cgtttgtgac aattgcgacc ggataattat
      721 attttactga attttccaga aaatacagta tactctttct gtaattttct gcacccagaa
      781 gaggctctcc gccgaatacg gtaatgtaag gacgcacctt ttcatctgca aatctgctgt
      841 tgatatgtga gaagaaactg tcagtgattt cctgatcgag catctttttt gaattctgat
      901 actcttcctg aaagcagtat ttgcaggcaa aattgcacac gaaagaagtg gagaagatca
      961 gctgtaccgg tgtcttctca tactcgtctg aaaattccag atacttctct ctgatcttct
     1021 cctcttcctc gtcctcattc ctgtaaatat accctctctc cagaagataa tcccgaagat
     1081 gataatcttc ccaggagccg gaagcaagcc tctgctgaaa ctcggaagct tcttcctttt
     1141 caatcagatc tattgagcct gaaagataat tgactgccat tatatccccg gacctgtatt
     1201 tctttgcaag aaaggtatat ttagaagttt tcatatcagt cgtgacatcc cgaaatgatt
     1261 tttgaggttt ttgcacagca ctgtgcgact gtttttgtct gcttttcaga cttcttctta
     1321 tctaaaggtt tcataggtaa accctccttt gattcagctt ttcatatttc tttttcaaat
     1381 aaatcatttc agcagttccc tccgccgcat ccgcatgttt ttttctgagc agatatctta
     1441 tgtccacgga ctgtaatact tcttacaatt actccgccct tttcataagg ctctgattct
     1501 tcgagaattt ccgcttcctt gaatccgccg gaagccaccg cagcaaagta ctcctgtttc
     1561 ggaacagctc cac
//
LOCUS       DAOQGJ010001131         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59380_length_1570_cov_2.606601, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001131 DAOQGJ010000000
VERSION     DAOQGJ010001131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59380_length_1570_cov_2.606601"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PK683_15760"
     CDS             complement(<1..734)
                     /locus_tag="PK683_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09952.1"
                     /translation="MDKEMSTNCQISINSIVQTADVSLSPEKKEIFFTPSSAGWPVNL
                     DTLSYLNLVNCKDAAGVSVVVQEKGAPVFIADGIIYLDGTNGLDTYTGLTTGDPLLTL
                     SAALAAVTSKCTGACTILMKGGVYPVSSSVIVPTNVSLFGGYDPSDWKKRRADKTSLA
                     PYDTIIDDLSSNVTGNINDPYATIKYSSYTGTKEKSVLDGIMVYSPFNVTAANYSTPI
                     AVVNLQSGAGISIRNVITLDRSNTAST"
     gene            complement(1138..>1570)
                     /locus_tag="PK683_15765"
     CDS             complement(1138..>1570)
                     /locus_tag="PK683_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09953.1"
                     /translation="FELLARNKIVLVKAGRGDDTDSLQVAGRVLDRNREKKMPEYSRI
                     IHLNIEISGEQITWDDFNENISSRMHTMLRHIDDVFGDNVTILTTYSYRDRKRFYPIV
                     TVDDSRISYSVNLLDGINSSMRFSNMALKSREALYAADKLI"
BASE COUNT          457 a          314 c          313 g          486 t
ORIGIN      
        1 gtggatgctg tattgcttct gtcaagtgtg attacatttc gaatgctgat cccggcacca
       61 gattgaagat ttaccactgc gataggagtg gagtaatttg ctgcagtgac attgaacggg
      121 ctgtagacca taattccgtc cagaacagat ttttccttcg ttcctgtata actggaatac
      181 ttaatcgttg cataaggatc gtttatatta ccggtcacat ttgaggacag atcatctatg
      241 attgtatcat aaggagcaag agaagtcttg tcagctcttc tttttttcca gtcagaggga
      301 tcatagcctc caaacagact gacgtttgtc ggtacgatga ctgatgatga aactggatag
      361 actcctccct tcatcaggat tgtacaggct cctgtgcatt tggatgtcac tgctgccagt
      421 gcggcagaaa gagtcaaaag aggatcacct gtagtaagac ctgtatacgt atcgagccca
      481 tttgttccat ccaggtagat tattccgtcc gcaataaaga ctggagctcc tttttcctga
      541 acaacaacag aaactccggc tgcatccttg cagtttacaa gattaagata actcagtgtg
      601 tcaaggttga ccggccatcc ggcggatgaa ggagtgaaga atatttcttt tttttccgga
      661 gaaagtgaaa cgtctgcagt ttgtacaatg ctgttgatgg aaatctgaca gtttgtggac
      721 atttccttat ccataaaaat acctaatttc tgagacttgg agataataac tggagataca
      781 ttatcccctg acataaaatc tgtggttaca attttagctg aaactgaatt tgaggacgca
      841 agaaaaactg cagagattat tccgaaaata cctgaatatt gcgtcggaga tcttttgaat
      901 ccgggctgac agtctgccat taagaaaata aaaatcggga tgtacaattt cctcagcata
      961 cccttattgc atatccgcct gcaggaaaag caagtaaaaa atttctgaac aaagcgaaat
     1021 gctgatttca ggagtatcat atatttaata acagttcaga ctgtcatatt ttcaataatc
     1081 tgaaattttt tcagttttag catctgttat gtgtaaaatg actgctcagt cattttgtca
     1141 tatcagctta tctgcagcat acaaagcctc tcggctcttg agagccatat tcgaaaatct
     1201 catggaagaa ttgattccgt ccaaaagatt gactgagtaa ctgattcggc tgtcatccac
     1261 tgttacaata ggatagaatc tttttctgtc acgataggaa tatgtggtaa gaatggttac
     1321 attgtctcca aatacatcat caatatgtct gagcattgta tgcattcttg aggatatatt
     1381 ttcattaaaa tcatcccaag taatctgttc tccgctgatt tcaatattca gatgaatgat
     1441 tctggaatac tccggcatct ttttttctct gttcctgtca agaactctcc cggcaacctg
     1501 aagtgaatca gtatcatccc ctcttcctgc tttcaccaga acgatcttat ttcttgcgag
     1561 aagttcaaaa
//
LOCUS       DAOQGJ010001132         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59400_length_1570_cov_1.861386, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001132 DAOQGJ010000000
VERSION     DAOQGJ010001132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59400_length_1570_cov_1.861386"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..563)
                     /locus_tag="PK683_15770"
     CDS             complement(174..563)
                     /locus_tag="PK683_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HNH09954.1"
                     /translation="MTAALHTAEINKTETGTEAKKYELPQGNFYESETGIRIELETPG
                     VSDKSVEILMDKEILTVRAERKDMIPEGYRLIHKEYDTFNGFERKFRITKEIDSEKSN
                     AKVKDGKIILSLVFREPLKRKIAVEAG"
     gene            complement(582..971)
                     /locus_tag="PK683_15775"
     CDS             complement(582..971)
                     /locus_tag="PK683_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH09955.1"
                     /translation="MEKIQRNMDEFWGSAGSYPFLGNYPMFNVYSKDDEILMIGQVPG
                     IKPEDLEITVKGREVSIKGQYKKEEKTELKAVRKEIREENFQRTFELAYALNENSVKA
                     VLKDGMLNITVLRAPEDKPKQISIIAE"
BASE COUNT          393 a          333 c          273 g          571 t
ORIGIN      
        1 ataggttcat atgtacaaaa aaaatgccgg ctgaaaatca gccagcacaa gtgtgaactt
       61 tggaaaggtt tcgtcgcaaa atttcttttg ccagccttca atgaaggcaa ttaacagagt
      121 aaaaacccct attctttttt caatcaaaat ttggggtttt gacaaaaaac ttttcaacct
      181 gcctcaactg cgatttttct ttttaacggt tccctgaata ccagactcaa aatgatcttg
      241 ccgtccttca ctttggcatt tgacttttct gaatcaatct cctttgtaat tctgaatttc
      301 cgctcaaaac cgttaaaagt atcatattct ttatggatca gtctgtatcc ttccggaatc
      361 atatcctttc tttctgcgcg aactgtcaga atttccttgt ccatcaagat ttcgacagac
      421 ttgtcactca ctccgggagt ttccagttca atcctgatgc cagtttctga ttcgtaaaag
      481 tttccctgag gcaattcata ttttttcgcc tcagttccgg tttctgtctt attgatttct
      541 gctgtatgca atgctgctgt cataatattg gtctccttga gttattctgc aattatgctg
      601 atctgttttg gtttgtcttc aggtgctctc aagacggtaa tattcagcat cccgtctttc
      661 aaaactgcct ttacactgtt ttcattgagt gcataagcga gttcaaaggt tctttggaag
      721 ttttcttccc tgatttcctt cctgactgcc ttcagttcgg ttttttcttc ctttttatac
      781 tgtcctttga tactgacctc tcttcccttt actgtgattt ccaagtcttc gggtttaatt
      841 ccgggcacct gtccgatcat cagtatttcg tcgtctttgc tgtatacatt gaacataggg
      901 tagtttccta aaaacggata acttcctgcg gaaccccaaa actcatccat atttctctga
      961 attttttcca tttcctgtaa aacgcctctg aaacctggtc tttggataaa cattgtcatt
     1021 ctcctttatc actctcattg ttcgagtgat ttatttttta tcactcaatt acttagagtg
     1081 ataatatgaa aaaaagttca agcacttttt tattttttta aaagattttt tttactgacc
     1141 tgactgagat tttacagaag accaagtgaa ttaaactggg aagtaactgg gttagaaaaa
     1201 gcataaagcc ttgaaggccg aggatttacc gtttcatatt ttaaaagcag gggcaataag
     1261 gacgaaaatt cctgccttgg cgaattgcct tttccttttt cctgatttct gttcagctga
     1321 ttctaaggtg ctgaagacgg tgattaattt tatttctgag atccttccag ggaaatgtat
     1381 tcagttttct cttttttcat tataataccc agatttttaa tgaatttttc atatagtcca
     1441 ttattcattg tctgaaaatg cctttgcatt gtcactgaat gtcgccgaaa ggcgagcaaa
     1501 cgcggggatt aggcagtttc cgagccgccg caaaggggtt tgcagcagtt tccaagtttg
     1561 cgaaacccct
//
LOCUS       DAOQGJ010001133         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59445_length_1569_cov_3.025760, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001133 DAOQGJ010000000
VERSION     DAOQGJ010001133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59445_length_1569_cov_3.025760"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1433)
                     /locus_tag="PK683_15780"
     CDS             complement(144..1433)
                     /locus_tag="PK683_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNH09956.1"
                     /translation="MNIDFRNRMQITQSELKEAEVLILFAADFKMRNSDVEYKFRQNS
                     DYFYHTGIEEADGILVLKKDWSAHFALPKDKEKEIWTGIRLGKDLIREKLNLNESFDL
                     AEWDTKKEEILTNTATLFHFFGQNPDRDRELLTLCSTLNRKLREGKFGPKRIEIPEFL
                     HERRLIKTAEEIEIIQRAVQITKNGHEKIMKICRPGMNEYELEAVLEEEYLKGGAWGG
                     GYGHIVAGGKNATVLHYNTNREILRDNDVVLVDSGAEVNYYTADVTRIFPVGKKFSTA
                     QSEIYSLVLEVQKKAILMTVEGNTVQEIHQETVRNLTEGLVSLSLLSGSVAENIENMN
                     YKKFYMHRTGHWLGMDVHDAGSYYVKGNPRPLQNGMITTVEPGLYFDPNDESIPAHYR
                     GIGVRIEDNVLVNGKNPVNLTAGIVKEISEIEALRNS"
BASE COUNT          361 a          377 c          283 g          548 t
ORIGIN      
        1 ggaatctgct tacggagact tcaggagact tcaggtttag agatcagagt tcagagcaga
       61 atgctgtttc attattttgc attctgcctt gattgcttca ggcaggacag tccctttctc
      121 cctgatccgg ggattctcct gcttcagctg tttctcagtg cctcaatttc ggaaatttcc
      181 tttacaatgc ctgcggtcag attgaccgga ttttttccgt tcacaagcac attgtcttca
      241 atccttaccc caattcccct gtaatgagca ggtattgatt catcattcgg atcaaaataa
      301 agtccaggct ccacagtcgt aatcattcca ttctgcaaag gtcttggatt gccttttacg
      361 taataactgc ctgcatcatg tacatccata ccgagccagt gacctgtcct atgcatataa
      421 aatttcttat aattcatatt ttctatgttc tcggcaactg atcccgagag caggctaagg
      481 gagacgagtc cttcagtcag gtttctgact gtttcctgat gtatttcctg tacagtattt
      541 ccttccactg tcataaggat cgcttttttc tgcacttcca gaaccagtga ataaatctct
      601 gactgagcag ttgaaaattt ttttccgaca ggaaagatcc ttgtcacgtc tgctgtgtaa
      661 taattcactt cagccccgct gtcaacaagt acaacgtcat tgtccctcag gatttcccgg
      721 tttgtattgt aatgcaggac agtggcgttt tttccgcctg caacaatatg accgtaacct
      781 ccgccccagg ctccgccttt cagatattct tcctccagaa cagcttcgag ttcatattca
      841 ttcattcccg gtctgcatat tttcattatt ttttcatgac cgtttttagt aatctgaacg
      901 gctctttgaa tgatttcgat ttcttctgca gtttttataa gccttctttc atgcagaaat
      961 tccgggatct caattctttt tggtccgaac tttccttctc tgagttttct gttcagcgtg
     1021 ctgcagagag tcagaagctc tctgtctctg tcggggttct gtccgaagaa atgaaaaagt
     1081 gtcgcagtgt ttgtcagtat ttcttctttt tttgtatccc attcggcaag atcaaacgat
     1141 tcattcagat tcagtttttc ccggatcaga tctttcccga gacgaatccc tgtccagatt
     1201 tccttttcct tgtctttcgg cagtgcaaaa tgtgcagacc agtctttttt caatacaagg
     1261 attccgtctg cttcctcaat tcctgtatgg taaaaataat ctgagttctg acggaattta
     1321 tactccacat cactgtttct cattttaaag tcagccgcaa aaagaataag aacttctgcc
     1381 tccttcagtt ccgactgagt gatctgcatt ctgtttctga aatcaatgtt cattctgaat
     1441 atcctttatt cttcctcttt aattttcttc aaaagtacgg caagtcattc aaaaaggtct
     1501 tcttcagtcc agtttctgaa tccgttcacc tgctttatgc agtttcagaa cttcggctgc
     1561 agtgatttt
//
LOCUS       DAOQGJ010001134         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59581_length_1567_cov_2.952381, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001134 DAOQGJ010000000
VERSION     DAOQGJ010001134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59581_length_1567_cov_2.952381"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PK683_15785"
     CDS             complement(<1..761)
                     /locus_tag="PK683_15785"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNH09957.1"
                     /translation="MIAVIGTGITGLSSAWILSRYFPVTLFEKHSVLGMAAFGTRFQA
                     GNTDLEFDIPFRTVKKDYYPLIFDIYRSAGIRTRKVDYSFRVESDKETVFGFRARSFI
                     GSTLGLPTIDSFRNRKGIRIFKDLIRFYTNSRKDFQQEKEPVSILNFLKKNSYSSEFI
                     YEFLLPTFALVNTCRTETVGNYPAETIIGYHSRGYGYTPQETAEFGTPDIVKRLSNSV
                     KNVRLGTEIRCIRLDGGRPVVETDAGSEKFDHVIVT"
     gene            complement(758..>1567)
                     /locus_tag="PK683_15790"
     CDS             complement(758..>1567)
                     /locus_tag="PK683_15790"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNH09958.1"
                     /translation="VPSDKEKKVLGFNIKPETFSFPELIKIKKSIETEGIEEIPGLEI
                     LKENNIKIKGVKDFKDFITRMELELVQGFADKSIPADELNNRFAIVTQLKSLLKDTHR
                     VLAAFGVEIHKRIASPVSCMIFFFLSFPLGLVVKRSGKGMSFTVAIVVLFTYYAVMII
                     CSGFSLKNQLPYFVGPWIANVLALSAGFYFMLTRTDIHPGNTAAGKLYKKIRNRIREK
                     ISPYHAKIMKKMEPFFGRAAVIRSKAEPVMERLKSALKKVKIKRTKKEEKS"
BASE COUNT          426 a          337 c          318 g          486 t
ORIGIN      
        1 gttacaatga catgatcaaa tttttcagag cctgcgtcgg tctctacgac aggacgaccg
       61 ccgtccagac ggatgcaacg gatttctgta cccaatctga cattttttac tgaattagaa
      121 agccttttta caatgtcagg cgtgccgaat tctgcagtct cctggggagt atatccgtat
      181 ccgcgactgt gataccctat aatggtttca gcaggataat ttcctacagt ctcagttctg
      241 caagtattca cgagtgcaaa tgtgggaaga agaaattcat aaatgaattc agatgaatag
      301 gaattttttt tcagaaaatt cagtatggaa acaggctctt tctcctgctg gaaatccttt
      361 ctggagtttg tataaaagcg aatcagatcc ttgaaaattc ggatcccttt tctgtttcga
      421 aatgaatcaa ttgtaggaag tccgagtgtg cttccaataa agcttctggc acggaacccg
      481 aaaacagttt ctttgtctga ttccactcga aaagagtagt ccacctttct tgttctgatg
      541 cctgctgatc tgtatatgtc aaaaatcaga ggatagtagt ctttttttac tgttctgaaa
      601 ggaatgtcaa attccagatc ggtattccct gcctggaacc ttgttccgaa tgccgccatt
      661 ccgagaacac tgtgcttttc aaacagtgta acgggaaaat accttgagag aatccaggca
      721 ctcgaaagcc ctgtgattcc tgttccaatt actgcaatca tgatttctcc tctttttttg
      781 tgcgcttaat tttaactttc ttcagtgccg atttcagtct ttccataacc ggttctgcct
      841 ttgatcggat cacagcagct ctcccgaaga acggttccat ctttttcatt attttagcat
      901 gataaggaga tattttttcc ctaattctgt ttctgatttt tttatataac tttccggctg
      961 cagtgttccc gggatgaatg tccgttcttg taagcataaa gtagaatcct gcagagagag
     1021 cgagaacatt tgcaatccag ggaccgacaa aataagggag ctggtttttc agagagaaac
     1081 cgctgcagat gatcatcaca gcataatatg taaacagtac aacaatggca acagtgaaac
     1141 tcattccttt tccggaacgt ttcactacga gtccaagagg gaatgaaaga aagaaaaaaa
     1201 tcatgcagga aacaggagaa gcgatcctct tgtgaatttc cactccgaac gcggcaagaa
     1261 cccggtgagt gtccttgaga agacttttca gctgagtcac aatggcaaac ctgttgttca
     1321 gttcatctgc agggatggat ttgtccgcaa atccctgaac aagttcaagt tccattctcg
     1381 taatgaaatc cttgaagtct ttcactccct tgattttgat attgttctct ttcagaattt
     1441 ccaggccggg aatttcctct attccttccg tttcaatgct cttcttgatt ttaatgagtt
     1501 caggaaagga aaatgtttcc ggcttgatat tgaatccgag cactttcttt tccttgtcag
     1561 acggaac
//
LOCUS       DAOQGJ010001135         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59656_length_1566_cov_2.450695, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001135 DAOQGJ010000000
VERSION     DAOQGJ010001135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59656_length_1566_cov_2.450695"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1566)
                     /locus_tag="PK683_15795"
     CDS             complement(<1..>1566)
                     /locus_tag="PK683_15795"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH09959.1"
                     /translation="NSELFELKENLEKKVQERTLELEKARQITEEANQELQDLNKFTK
                     LINSAKDLDYILNQIYDYIRKNMELGIFWLILYNNEKNELYNYKWILPEDNDWDEDQK
                     RFLTDFRISVNPGIGTLYQTFASREIFYLPDIYKSVSGSKNHFINEFNGKIYFSKRLD
                     LKILTVGKFESFVQIPLTVNDRTIGILNLAPIDRKTSLERRELFSLIQFGEQIAGVIQ
                     NMHLLEETEKARQESALQKQLAEKTKEELEVLNSFNRMINELSDLDHIFTEISKYIYQ
                     RYNIEDVWLLLADEKKKELFPLRSFERTLLSEAKMNFKNEFRIPLNENGGLNYIQYRR
                     QKPLYVQKIKNIPHPTDRFIIENLEVKSFLNVPMLIQGNTVGIVYFSNSTKEFHLSKK
                     EISSISGFCQQIAGVIHNSHLLKETEDAKKEAEKEREKSDRLLLNILPIQVAEELKNK
                     GRAEPVFYESVTVLFTDFKGFTVIAEKMSPAELIKELDGCFSQFDKITERYKLEKLKT
                     IGDSYMCAGGIPAV"
BASE COUNT          386 a          337 c          294 g          549 t
ORIGIN      
        1 gactgcgggt attcctccgg cacacatata gctgtctccg atagtcttca gtttttcaag
       61 tttgtatctt tctgtaattt tatcaaactg gctgaaacat ccgtccagtt ccttaatgag
      121 ctcagcagga gacatcttct ctgcgattac agtaaatccc ttaaaatcag tgaacaatac
      181 tgtcactgat tcatagaata caggctctgc tcttccttta ttcttaagtt cctctgcaac
      241 ctgaatcggc agaatgttca gaagaagtct gtccgatttt tctctttcct tttctgcttc
      301 ctttttggca tcttccgttt ctttcagaag atgagagttg tgaatgactc ccgcaatctg
      361 ctgacagaac ccgctgatgc tggatatttc cttcttagaa agatgaaact cctttgtgga
      421 attgctgaaa taaactatgc ccacagtatt tccctgaatc agcataggca cgttcagaaa
      481 ggactttact tcgaggtttt caatgatgaa tctgtcagta gggtggggga tattttttat
      541 cttctgcaca taaagaggct tctgtcttct gtactgaatg taattgagtc ctccgttttc
      601 attcagcggg atcctgaatt cattcttgaa attcattttt gcttcggaga gcagagttct
      661 ttcaaagctt ctcagtggaa aaagctcctt ctttttttca tcggcaagca gaagccatac
      721 atcctctata ttgtatcttt gatatatgta ttttgaaatc tctgtgaata tatgatccag
      781 atctgaaagc tcattgatca tcctgttgaa actgttcagg acttccagtt cctccttggt
      841 tttttctgca agctgcttct gcagagcaga ttcctgtctg gccttttctg tttcttcaag
      901 aagatgcata ttttgaatca ctcctgcaat ctgctctccg aactgaatca ggctgaaaag
      961 ttcccttctt tccagggaag tttttctgtc gatcggggca agattcagta tgccgatcgt
     1021 ccggtcgttt acagtcagtg ggatctgaac gaaggattca aattttccga cagtgagtat
     1081 tttcagatcc agccttttgg aaaagtagat ttttccattg aactcattga tgaaatgatt
     1141 tttgcttccg gaaacggatt tgtaaatgtc cggcagataa aaaatctctc tggatgcaaa
     1201 tgtctggtaa agagttccga ttcccggatt cacagaaatc ctgaaatctg tcagaaatct
     1261 tttctgatcc tcgtcccagt cattatcctc cgggagtatc catttgtaat tataaagttc
     1321 atttttttca ttattataca gaatgagcca gaatattccc agttccatat tcttacggat
     1381 gtaatcgtaa atctgattca gaatgtaatc cagatccttt gcggaattga tcagttttgt
     1441 aaacttgttc agatcctgaa gttcctgatt tgcttcttca gtaatctgtc tggccttttc
     1501 gagttcgagc gttctctcct gcaccttctt ctcgagattc tccttcagtt caaacagttc
     1561 tgaatt
//
LOCUS       DAOQGJ010001136         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59702_length_1565_cov_3.325166, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001136 DAOQGJ010000000
VERSION     DAOQGJ010001136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59702_length_1565_cov_3.325166"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..761)
                     /locus_tag="PK683_15800"
     CDS             complement(69..761)
                     /locus_tag="PK683_15800"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNH09960.1"
                     /translation="MKITVSAVIILTVGCSSSVPASLRSATEIKNDSVKLSAPKNLPP
                     KASPGQCFSKIVIPAEYKTVDDQVMVSPRRTKMERIPAETETVDVKVIDVPGRTFWKA
                     SSDDEVYYLDSEPPVYKTVKKEIIRKPAYVKTTEIPAEYKTVTRKILVSPEKTEWEEV
                     LCGNNAQEAVIWQIQKTLKEKGYNPGNFEGKLDSATLNAVNEYQRKNGLKVNTNGLIN
                     METARSLGVKLQ"
     gene            884..>1565
                     /locus_tag="PK683_15805"
     CDS             884..>1565
                     /locus_tag="PK683_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH09961.1"
                     /translation="MKKYSILTLIIIVLLIQCRTAASVKTPAPETEQKHISEKGWNST
                     HEAAASGNPESLKKISSDRPSITGKDASGRTPLHIAAMKGYPESARILAESGADLNAK
                     DSRGWTALHWAAFEGRSEAAGMLIDSGAEVNAPDSENGWTALHWAAFEGRTEIIKLLI
                     KSGADRNSRDSSGRTAVHLAASKGYTETLKLLISEKTETRDSNGWTPLHWAAFEGRTE
                     TEGSIRYCP"
BASE COUNT          427 a          349 c          331 g          458 t
ORIGIN      
        1 attttctgac ttatccgatt cctgctccgt gcagaaaaaa ttccgcacgt tttcagcttt
       61 tcggtggatt attgaagttt tactccaagc gaacgtgctg tctccatatt gatgagaccg
      121 tttgtattta ccttcagacc gttttttctc tggtattcgt ttactgcatt cagggtggcc
      181 gagtccagct ttccttcaaa gtttccagga ttgtatcctt tctccttcaa ggttttctgt
      241 atctgccaga tgactgcttc ctgagcattg ttaccgcaga gaacttcttc ccattctgtc
      301 ttctcgggac tgacaagaat cttccttgtc acggtcttgt actctgcagg aatctccgta
      361 gtcttcacat aggcaggttt tcttataatc tcttttttta cagtcttgta tacaggaggt
      421 tcagaatcca gataatatac ttcatcatcg gaggaggctt tccagaaagt tcttccggga
      481 acatcaatca cttttacatc cacagtttcc gtctctgccg gtatcctttc cattttggtt
      541 cttcggggtg aaaccatcac ctgatcatcc actgtcttgt attctgcagg aatgacaatt
      601 ttggaaaaac actgaccagg cgatgctttc ggaggaagat tttttggtgc actcagttta
      661 acactgtcat ttttaatttc ggttgccgat ctgagagatg ccggcacaga agatgaacag
      721 ccgactgtta gaataatgac ggcagagact gtaattttca aaaacatata aactcctgcc
      781 taaatcattt tttctctgtc atttttgtat actttttttc ttttcaaaac ggtgatttca
      841 tatatactgg tctaattatt ttacctgtat cggatactcc tctatgaaaa aatattctat
      901 attgactctt attatcattg tgcttttaat tcagtgcaga actgctgcat cagtcaaaac
      961 ccctgcacct gaaactgaac agaaacatat ttcagaaaaa gggtggaact ccactcatga
     1021 agcagctgca tcaggaaatc cggaatcatt aaaaaaaatc tcatcagaca gaccctcgat
     1081 caccggaaag gatgcttccg gaagaactcc tctgcatatc gcggcaatga aagggtatcc
     1141 ggaatctgca agaattctcg cagaaagcgg tgcagatctg aatgcaaaag acagccgagg
     1201 ctggactgca ctccattggg cagcattcga aggaaggtct gaagcggcag gaatgctcat
     1261 tgacagcgga gctgaagtga atgcgcctga ttctgaaaac ggatggacag ctcttcactg
     1321 ggcagcattt gaaggaagga ctgaaataat aaaactcctt ataaaaagcg gggccgacag
     1381 aaacagcagg gacagttcag gaagaactgc agtgcatctt gctgcttcca aaggttatac
     1441 tgagacactg aagcttctga tttccgaaaa aacagagaca agggacagta acggatggac
     1501 ccctctgcac tgggcggcat ttgaaggaag aactgaaaca gaggggtcca tccgttactg
     1561 tccct
//
LOCUS       DAOQGJ010001137         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59729_length_1565_cov_2.238411, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001137 DAOQGJ010000000
VERSION     DAOQGJ010001137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59729_length_1565_cov_2.238411"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..641
                     /locus_tag="PK683_15810"
     CDS             225..641
                     /locus_tag="PK683_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09962.1"
                     /translation="MKLILRNVIAVLIGFAVGSAVNMGLIMISGSIIPPPAGADVTTM
                     EGLKASLHLFEPKHFLFPFLAHSLGTFAGALCASFIVLNRRMEFALGVGILFLLGGMA
                     NVFMLPSPAWFTALDLICAYIPMGYLAGKLALKKNN"
     gene            complement(759..1286)
                     /locus_tag="PK683_15815"
     CDS             complement(759..1286)
                     /locus_tag="PK683_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09963.1"
                     /translation="MKKTEPFANGENRRVYRCRQDFSEAESVLHIQYKDIGALMNTFQ
                     IEKDIPVVYETADSFPNGIQAAFDSLHKKLGDKKQRKIFGMSRPEGKKGIVYRACAEE
                     LFPGEAEKLALPKLVIQAGNWYCTEIKDVMKDPSQIEEAFREYIYKPDIDPDGYCVEL
                     YSEDNRDVKCLVRKK"
BASE COUNT          425 a          324 c          315 g          501 t
ORIGIN      
        1 tgactaaatt aaaaaaaaaa ataaaaataa ttatatatat ttaataatta ttttatgccc
       61 tttcagactg ttttctgaat caaaaaataa tctgcgtctg aatattcttt gacgacgata
      121 tccgtatgga tgtcttactc ttgttcagat ctcccgcatc ctttaggtga aaatttattg
      181 cagattgaaa caggaaacag aaaatgccat cgtaggagaa aaatatgaaa ctgattctga
      241 gaaacgttat cgctgtattg atcggttttg cagtcggaag tgcagtcaat atgggcctca
      301 ttatgatcag cggctccatc ataccgcctc cggcaggtgc tgatgtgact acgatggagg
      361 gtctgaaagc aagccttcat ttatttgaac cgaaacattt tttatttccg tttctggctc
      421 actctcttgg aacttttgca ggagcactct gcgcttcctt cattgtgctg aatcgcagaa
      481 tggaatttgc acttggggta gggattctgt ttctgctcgg cggtatggca aatgttttta
      541 tgctcccgtc tcctgcctgg tttactgcac tcgatctgat ctgtgcctat attcctatgg
      601 gataccttgc gggaaaactg gctttgaaaa aaaataatta gaacatcatt ataaagatca
      661 gcggtcagcc ttaaaaaaag agaccgcttg aactggactg agaagatttg cagctgcttt
      721 tgaagaaaac ttctgcatac atttattatt gcaggatttt atttttttct gacgagacat
      781 tttacatccc gattgtcttc ggaatacaat tcaacacagt aaccgtcagg atcaatatcc
      841 ggcttgtaaa tatactcccg aaacgcttct tcgatctgtg agggatcttt cattacatcc
      901 ttgatttcag tgcagtacca gtttcccgcc tgaatcacaa gtttaggaag tgccagtttt
      961 tctgcttctc cggggaaaag ctcttccgcg caagccctgt agacgattcc cttttttcct
     1021 tccggtctgg acattccgaa gatttttctc tgttttttat caccgagttt cttatgaagg
     1081 gaatcaaagg cagcctgaat tccgtttgga aaactgtccg ctgtttcata aacaacagga
     1141 atgtcttttt ctatctgaaa ggtattcata agtgcaccta tgtctttgta ttgaatatga
     1201 aggactgact ccgcttctga aaaatcttgt ctgcatctgt aaactcggcg gttctctccg
     1261 ttcgcgaaag gttctgtttt cttcatcaga attttacaga tcacttcagt tgcatactgc
     1321 acggctcagt gaatgagtca ataaatatta ttttttacga agcaatatag aaattcagca
     1381 gtttaacacc ttgaataaat cggtgaccgg cggtgaaaat tgtatgcttc gatgtgaccc
     1441 ttcgagacag ctcaaggcat cgctcagcac aagtcgaggc atcattccgc ataacatata
     1501 tgaaagtcga ttgctgattg tacggcattg gccgacaggc atttctgtcc atacatctgc
     1561 aggtg
//
LOCUS       DAOQGJ010001138         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59782_length_1564_cov_2.727634, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001138 DAOQGJ010000000
VERSION     DAOQGJ010001138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59782_length_1564_cov_2.727634"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PK683_15820"
     CDS             complement(<1..800)
                     /locus_tag="PK683_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HNH09964.1"
                     /translation="MRSAGTMVLPMAEFYHKYANIIDLIMMPFTYGFVGWFTNWVALK
                     MTFYPIRFWGIYPPYLGWQGIIPRKAYKMASKSVDIITGRLLKIEEVFDKVEPEGIEK
                     EMRPLVEEMIQESTRDIIEQINPAIWVMLPENVKADIYKNSKAQVPESFRQIIYELRK
                     NILQVFDLKGMVLKKLSGDNVKLVVDMFQSVGGPEFRFIEVSGFYFGVVLGSIQAVIW
                     NFYPEWWTLPIQGIIVGYLTNYLALEMIFRPYEEKQYLFFKYQGLFLK"
     gene            901..>1564
                     /locus_tag="PK683_15825"
     CDS             901..>1564
                     /locus_tag="PK683_15825"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH09965.1"
                     /translation="MSNQFRTLNEMFLLRVMEFGDKPTLFSKDSQKNFKGTTLREAYT
                     DAENAGLGLMDLGLEPNDKVGLMSDNRLEWFTADLAVLLNGAANVPRGSDSTAQEIQY
                     ILEHSESKFCFIEHEKLLKQVLPIADKTPVKKFIVLDRAFKSGGDERVLSLSDLIEKG
                     RTLRTTKLAELIKRAKAVKEDDLFTIIYTSGTTGMPKGVMLSHRNMVYNVKMVPPMVD
                     VIK"
BASE COUNT          459 a          342 c          331 g          432 t
ORIGIN      
        1 cttttcagaa acagtccctg gtatttgaag aacagatact gcttttcttc atagggacgg
       61 aaaatcattt cgagagcaag atagtttgtc agataaccga caatgatacc ctgaattgga
      121 agagtccacc attcaggata gaaattccag atgactgcct gtatgctccc gaggacgacc
      181 ccgaaataaa atccggaaac ctcgataaat cggaattccg gtcctcctac gctctgaaac
      241 atatctacga caagttttac gttgtctccg gagagctttt tcagcaccat ccctttcaga
      301 tcgaatacct gcaggatgtt ttttctcagt tcataaataa tctgacgaaa ggattcagga
      361 acctgtgcct tggaattctt atagatgtcg gccttcacat tttcaggaag catcacccag
      421 atagcaggat tgatctgttc aataatgtct ctggtggatt cctggatcat ttcttctacc
      481 agaggacgca tttccttttc tatcccttcc ggctccactt tgtcgaagac ttcttcgatt
      541 ttcagaagcc ttccagtgat gatgtccact gatttggaag ccattttata tgcctttctg
      601 ggtataatcc cctgccagcc cagataagga ggataaattc cccagaaccg gatcgggtaa
      661 aaagtcattt tcaaagccac ccagttggtg aaccagccga cgaaaccgta tgtgaacggc
      721 atcatgatca aatcgatgat gtttgcatat ttatgataga actctgccat tggaagaacc
      781 atagtacctg cacttctcat tgtgaaaagc agaatctttt tttcgattaa aaatttcttt
      841 tcaaatctgt ctgcctttcc aaacttcgta ccgagattat ttttttaaca gggatgactt
      901 atgagcaatc agtttagaac tctgaatgag atgtttcttc tcagggtaat ggaatttgga
      961 gacaaaccga ctctcttctc aaaagacagt cagaagaatt ttaaaggaac aactctcaga
     1021 gaggcctata cagacgctga aaatgcaggt ttgggactta tggatctggg tcttgaaccg
     1081 aatgacaaag tgggcctgat gtccgacaac agactggaat ggtttacagc cgacctcgca
     1141 gttcttttga acggtgcggc aaatgttccg agaggatcgg attcgactgc tcaggagata
     1201 cagtatatcc ttgaacacag cgaaagcaaa ttctgtttca ttgaacatga aaaacttctg
     1261 aagcaggttc ttcctattgc agacaagact cctgtaaaga aattcattgt tctcgacagg
     1321 gctttcaaat ccggaggaga tgaaagagtg ctctcccttt cagatctgat agaaaaagga
     1381 agaacactca gaacgactaa actggctgaa ctcatcaagc gtgcaaaagc agtcaaggaa
     1441 gacgatctgt tcaccatcat ttacacttcc ggtacaacag gtatgcctaa gggagtgatg
     1501 ctgtcccaca gaaatatggt atacaatgtc aagatggttc cgccgatggt ggatgtcatc
     1561 aagg
//
LOCUS       DAOQGJ010001139         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59810_length_1564_cov_2.037111, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001139 DAOQGJ010000000
VERSION     DAOQGJ010001139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59810_length_1564_cov_2.037111"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PK683_15830"
     CDS             complement(<1..668)
                     /locus_tag="PK683_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09966.1"
                     /translation="MRLLFFCTVFQIISLLPLYSSDWDDLEEGENEKVYGSERSKKYP
                     RSNLFYEMEKWENHYSVKALGIYRHYDYPKFESRTFFPFYHRLTSRIDSREKYRILNF
                     SDTKNLSSRHKSFFPFVFWGEDKRDEYHSVFPLYYRSYSKPEDSEKRSSFFFAPFPMF
                     YRNRSYAVHSENTHRETLHWNLFFYSRKAYDESAVSDRTHSSLLGFPLIPVLFYTNYN
                     EGTGT"
     gene            complement(808..1353)
                     /locus_tag="PK683_15835"
     CDS             complement(808..1353)
                     /locus_tag="PK683_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002177926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH09967.1"
                     /translation="MKKAAIILGVLLSLILILAVISPNDFILERTTVISKPKDKVFSE
                     LKFLKNHEKWNAWSKKDPKMKKEFKGTDGAPGFISSWESEHEEVGTAEQEILNITEGQ
                     KIETQIRFKKPFTANFMSYIILESSGENSTKVLLGMSDKMKFPMTVISFIVNVCLGNQ
                     NKIISNMDESLKNLKTLLESE"
BASE COUNT          415 a          314 c          321 g          514 t
ORIGIN      
        1 gttcctgtcc cttcattata atttgtataa aaaagtaccg gaatcagagg aaagccgagc
       61 agggaggagt gagttctgtc cgagactgcg gattcatcat atgcttttct gctgtaaaag
      121 aaaagattcc agtgaagggt ttctctgtga gtgttctccg agtgcactgc ataggatctg
      181 tttctgtaga acattggaaa gggagcaaaa aagaaactgc ttcttttttc actgtcttcc
      241 ggtttggaat acgatctgta atagagagga aatacagaat ggtattcatc tcttttgtct
      301 tctccccaga atacgaaagg gaaaaaggac ttgtgtctgg aactcagatt tttcgtatca
      361 ctgaagttca gtatcctgta tttttctctg gaatctattc tgctcgtgag tctgtgataa
      421 aaagggaaaa atgtacgtga ttcgaattta ggataatcat aatgcctgta aatacccagc
      481 gcttttacgg aatagtggtt ctcccatttt tccatttcgt aaaacagatt gcttctcgga
      541 tactttttgc ttctttcgct tccgtaaact ttttcatttt cgccttcctc cagatcatcc
      601 cagtccgaag agtacaaggg cagtaaagag atgatctgaa aaacagtgca gaagaataag
      661 agtctcatac gaaaacattc ttccctgtct caatgtatgt caaggaaact ttggcgcggc
      721 tccgttcaga aactgttcct gtcattccgg aaatgaactg aggcagaaac tgagtttcgg
      781 taaacggatt caagactcag atgcggatta ttccgattcc aggagagtct tcagattttt
      841 cagactttca tccatattgc tgatgatttt gttctggttt cccaggcata cattgactat
      901 gaagctgatg acagtcatcg gaaatttcat tttgtcgctc atccccagaa gaacctttgt
      961 gctgttctcc ccggaagact ccagaatgat gtacgacata aaatttgcag tgaagggctt
     1021 tttaaacctg atctgtgttt ctattttctg tccctctgta atattcagta tttcctgttc
     1081 tgctgttccg acttcttcat gttcgctttc ccaggatgag ataaacccgg gagctccgtc
     1141 tgtacctttg aactctttct tcattttggg atctttcttt gaccacgcat tccatttttc
     1201 atgattcttc agaaacttaa gttcgctgaa gactttgtct ttgggtttag atatgacagt
     1261 tgttctttcc agaataaaat cattgggtga gatcactgcc aggataagaa ttaatgataa
     1321 aagtacgcct aaaatgatcg ctgctttttt catggaaaac tcctttctga atgatccgtt
     1381 cagagtatga caggattatt ttctgtcaat aaaaaagtga gtcactcagg cactttcacc
     1441 catccgtaaa gaatgtatgt ttttctcaga agttttgcaa atgcctttct gttcaggtat
     1501 ttccaggcaa gaagctgagt ggcatatctg atgtcttcgg actcctttac agggaacact
     1561 tcgg
//
LOCUS       DAOQGJ010001140         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59847_length_1563_cov_3.124005, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001140 DAOQGJ010000000
VERSION     DAOQGJ010001140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59847_length_1563_cov_3.124005"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..>1563
                     /locus_tag="PK683_15840"
     CDS             104..>1563
                     /locus_tag="PK683_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNH09968.1"
                     /translation="MKIEKETTRKLLEYYSELYHFPLERNRIFMMLNDFSDAAEKYSA
                     FLNQFKVPFHFSFKPLDMDSAESLGKTDWIFRDGVFCRIIFYAPYYILTEDFDGHRKW
                     YRKSDFFRKEEIYFLSAEPFRPFTLGEKEISPEKRILSLIRLESSDIGLLTVFSIFAA
                     LLSLVIPVGTQSFVNILAFGTQMQPVIVLTFLITGALTFAGVLKILQMTAAEILQQRL
                     FARVFTEIIAGFPESDKDSFLKTENSRKLNYLLDISTAQKSATVLLTDGLAILLQMTV
                     GIIVLLLYHPIFIIYDVFIIILCYFVTVRLGKDGVSTSILESKYKHKTVFWLEEAFEK
                     FSAIQGRRGLDLVRKKAEGIAGDYLHYRGVHFHTLKKQASVFILFQAVGTAVLLGMGG
                     YLVIKGEISLGQFVASEIIAAKIMESFSKSAKYLESYYDLCASLDKISHALESGDSSF
                     RRNTSAASNDDRMEFRNASFSVSGKTFLVNDILMNKG"
BASE COUNT          448 a          306 c          328 g          481 t
ORIGIN      
        1 ctctcatttt gttttttcag accgcacatt tttttaataa aatcagcctg aaaaataaaa
       61 atctgttgac taataagaat tagaatcatt ctaaatgaat aggatgaaaa tagagaaaga
      121 aacaaccagg aaactgctcg aatactattc agaattatat cattttcctc tggaaagaaa
      181 caggattttt atgatgttga atgatttttc tgatgcagca gaaaaatatt cggcattcct
      241 gaatcagttt aaagttcctt ttcatttcag ctttaaaccg ctggatatgg acagtgcaga
      301 gagtcttgga aaaactgact ggatatttcg cgacggagta ttctgccgaa ttatattcta
      361 tgccccatat tatattctga ctgaggattt tgacggacac aggaaatggt acagaaaatc
      421 ggattttttc cggaaggaag aaatttactt tctaagtgct gaaccgttcc gcccctttac
      481 tctcggagaa aaggaaatca gtcctgaaaa aagaattcta tctcttatcc ggctggaatc
      541 ttccgatatc ggtcttctga ctgtgttttc catatttgcg gcacttctgt cccttgtcat
      601 tccagtaggc actcagtcat ttgtgaacat acttgcattc ggaacgcaga tgcagcctgt
      661 cattgtactg acctttctga tcacaggtgc acttaccttt gcaggcgttc tgaagattct
      721 gcagatgaca gcagccgaga ttcttcagca gaggcttttt gccagggtat tcactgagat
      781 cattgcaggg tttcctgaat cggacaagga cagttttctt aaaacggaga acagcagaaa
      841 gctcaactac cttctggata tttccactgc tcagaaaagt gctaccgttc ttctgactga
      901 cggtctggca atccttctcc agatgacggt cggcattatt gtccttcttc tgtaccatcc
      961 aattttcatt atatatgatg ttttcatcat tattttgtgt tattttgtaa cagtccgact
     1021 gggaaaggac ggagtcagca ccagcatcct tgaatcaaag tacaagcata agaccgtttt
     1081 ctggcttgaa gaagcttttg aaaaattttc tgccattcag ggaaggagag gacttgatct
     1141 ggtcagaaag aaggctgaag gaattgcagg ggactacctt cattacaggg gagtgcattt
     1201 tcataccctt aaaaaacagg cctctgtctt cattcttttt caggcagtag gaacggcagt
     1261 tcttctcgga atgggaggat atcttgtcat taagggagag atttcgcttg gacagtttgt
     1321 cgcatcagaa atcattgctg caaaaattat ggagagtttc agcaagtctg cgaagtatct
     1381 ggaaagttat tatgacctct gtgccagtct ggataaaata tctcacgccc ttgaatccgg
     1441 ggattcttca ttcagacgaa atacgagtgc agcctcgaat gatgacagaa tggaattcag
     1501 aaatgcatcc ttttctgttt ctggaaaaac tttccttgtt aatgatattc tgatgaacaa
     1561 agg
//
LOCUS       DAOQGJ010001141         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_59851_length_1563_cov_3.023210, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001141 DAOQGJ010000000
VERSION     DAOQGJ010001141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59851_length_1563_cov_3.023210"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="PK683_15845"
     CDS             complement(<1..1414)
                     /locus_tag="PK683_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09969.1"
                     /translation="MRYSIFLFSILLCAAGFLNLQTERLADLDFDTSYVLYGLMFDRQ
                     ILSINPPILHPAISLAYFPFQVFLSAGIHPMTSLQLQYGIYLGLSVILFGLLCYRLTE
                     RGIFSFIMAGLFSFFPSIQIIIQTMDDNIYSYPFLFTGLILAFPAAKKKASDIYLRFL
                     GCVIIFSFMVFISMTSFLWVFFLSFLFLLNIFTSLSVKEGLKLLIPVILTGIICTAGF
                     FAFFSSFHSLPIKDFVRETLGFSQNFSSVNSLLLENFSKLEYIKFSLWQGLFSVPRSE
                     TAASPLSHMLSFITAKSKTAYPLIYLVFYYLFILKLFIGFRKKKNAEDFSHAANLLLF
                     LVLNLFFLLWYKEDSSFHERWDFFYLTFPWTALLVFRSRKKTDAVFFFLLLSVPLYFG
                     ARIFYHSKDNYSDFRSVKKNEGKYEMYVFTQDEVISPSSSMVHILKDYRPLKIVKMAE
                     KPRYEAHLYPFFLISKEAFLQ"
BASE COUNT          547 a          285 c          343 g          388 t
ORIGIN      
        1 cctgaagaaa tgcttccttt gagatcagaa aaaaagggta aagatgagct tcatatctgg
       61 gtttttccgc catctttact attttcaacg gtcgatagtc cttaaggata tgcaccatac
      121 tgctcgaagg actgatcact tcatcctgtg taaagacata catttcatat tttccttcat
      181 tcttttttac gctgcggaag tcggaataat tgtcctttga atgataaaat atcctggctc
      241 cgaaataaag aggaacagac agcagcagga aaaaaaatac tgcatcagtc ttctttctgc
      301 ttcgaaatac aaggagtgct gtccagggaa aagtcagata gaaaaaatcc caccgctcat
      361 gaaagcttga gtcctccttg taccagagca gaaaaaaaag attcagaaca agaaataaaa
      421 gaaggtttgc tgcgtgagaa aaatcttcag catttttttt ctttcgaaag cctataaaaa
      481 gttttaaaat aaaaagataa tagaacacca gataaatgag aggatatgct gttttcgatt
      541 tagctgtaat gaacgagagc atatggctca gaggtgatgc agctgtttct gagcgaggaa
      601 cggaaaaaag accctgccat agagaaaact ttatgtattc cagttttgaa aaattttcaa
      661 gaagtaatga atttactgat gaaaaattct gactgaagcc taacgtttcc cggacaaagt
      721 ctttgatcgg aagagaatgg aaagaagaaa agaatgcaaa gaatcctgct gtgcagataa
      781 ttcccgtcag gattacagga atcaggagtt tcagaccttc cttcacggaa aggctggtaa
      841 aaatatttaa gagaaaaaga aaggacagaa agaataccca aaggaaggag gtcatagata
      901 taaagaccat aaagctgaag ataatcacac atcccagaaa tctgagatat atatctgagg
      961 cctttttttt cgcggcaggg aacgcgagaa taagtcctgt gaacaggaac ggataggaat
     1021 aaatattgtc gtccattgtc tgaatgatta tctgaatgga tgggaagaaa ctgaacaatc
     1081 ccgccataat gaaagaaaat attccccttt ccgtgagtct gtagcaaagc agtccaaata
     1141 aaatgacgga aagtccaaga taaatcccgt actgcagctg aagagatgtc attggatgaa
     1201 ttcctgcact gagaaaaacc tgaaaaggaa agtatgccag gctgatggca gggtgaagaa
     1261 tcggagggtt tatactcaga atctgtcggt cgaacataag tccgtacaga acatacgatg
     1321 tgtcaaaatc gagatcggca agccgttctg tctgaagatt taagaatccg gcagcacaca
     1381 gaagaatcga aaaaagaaat atagaatatc tcacagcttt ctgatttaga atctgcttaa
     1441 ctgtctgaag catgctgcat agactgcagg atttgtccct gcctgaaaat ggggaggtgg
     1501 ctgttgttga tacggagaac tttgccatca aacaatacat ttccagcggc gggcgccccc
     1561 ccc
//
LOCUS       DAOQGJ010001142         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60009_length_1561_cov_2.316733, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001142 DAOQGJ010000000
VERSION     DAOQGJ010001142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60009_length_1561_cov_2.316733"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1561)
                     /locus_tag="PK683_15850"
     CDS             complement(<1..>1561)
                     /locus_tag="PK683_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09970.1"
                     /translation="PAKAKLDASLTFEEALFIVAVGNGLKGMMGLKPNCGQFNTLSDC
                     GAYNGVWNSDVSAVKSMKLKQYDALGTSPTTANFSPTPGDSVLLLEGWGYGAAGTNPS
                     LGLAFVLPNTAYNLLVPTPSLVFGMIPPAISQNFDALERLGFLTDQVVTPSQVPNNWD
                     KRNKLAPVIAALAKTFDDQVDVANSKNPFTLLTSLTKILARPNVLTEQDPDSFNTAMV
                     FPACTGGCPPTIPSIRIQGSSSTTGIRNPSALSSEYYPKKNLRPMISVLSENQRKFQD
                     GMLNLVAKTDLLTNTIRTLAFLGSSSRTAAREKIFNGLYKIMDNIRIDSDQTSTTLQY
                     NLQTYIKEVRDRIAVYPDSRNTSDLTGSSWDGVSDSVAFIRDYLAKASPYTLVGSIDF
                     SLGMLADIPPSSEQITSLLNVVSVMFTDTAGNRKYRVLSMMTGDLPVILQQVAPYGRN
                     VFALAGSLGKPGSYFSYLEQKMTIGSYRIRDLLDDVNRFCTAEMVQTKLENSDALFYS
                     SGKLLKLFADVY"
BASE COUNT          394 a          331 c          378 g          458 t
ORIGIN      
        1 ataaacgtcg gcaaacagtt tcagaagttt gccgctggaa taaaaaagtg catccgaatt
       61 ttccagtttt gtctgaacca tttctgcagt gcagaatctg ttcacgtcat cgaggagatc
      121 tctgattctg tagcttccga tggtcatttt ctgttcaaga taactgaaat aacttcctgg
      181 tttacccaaa gagcctgcaa gtgcaaatac gtttcttccg tacggcgcta cctgctgtaa
      241 tataaccggc agatctcctg tcatcatact cagtactctg tatttcctgt ttcctgcagt
      301 atcggtgaac attacagata ctacatttaa taatgatgtg atctgctcgg aactcggagg
      361 aatatcggcc agcattccca gtgagaaatc aatgcttcct acaagagtat acggtgatgc
      421 ctttgccaga tagtcccgga taaatgcaac ggaatcactg actccgtccc aggaggagcc
      481 tgtgagatcg gaagtgtttc tggaatcagg ataaactgca attctgtcac ggacttcctt
      541 gatgtaggtc tgaagattgt actgaagggt agtggatgtc tggtctgaat caatccggat
      601 attatccatt attttatata gaccgttgaa tattttttcc cgtgcagctg ttctggaact
      661 gcttcctaaa aatgcaagtg ttctgattgt atttgtcaga agatctgttt ttgcaacaag
      721 gttcagcatc ccatcctgga actttctctg gttttcagaa agtacgctga tcattggtct
      781 taaatttttt ttgggatagt attcactgga aagagcagat gggttcctga ttccggtggt
      841 ggaagaagat ccctgtatcc tgatgcttgg aatagtagga ggacaaccgc cggtgcaggc
      901 agggaatacc attgctgtat tgaaggaatc cggatcctgt tctgtaagaa catttggtct
      961 tgccagaatt ttcgtcagac tggtaagcag agtaaacggg ttcttgctgt tggccacatc
     1021 tacctggtca tcgaatgttt ttgccaaggc agcaatcact ggggccaatt tgttcctctt
     1081 gtcccagttg tttggaacct gagaaggagt gactacctga tctgtaagaa atccaagtct
     1141 ttccaatgca tcaaaattct gtgaaatggc aggtgggatc attccgaaga caaggcttgg
     1201 ggtaggtacg agcagattgt atgctgtgtt tggaaggacg aatgccagtc ccaatgaagg
     1261 gtttgttcct gcagctccat aaccccagcc ttccaggagg agaacagagt ctcccggtgt
     1321 tggggagaaa ttggcggtcg taggactggt gccgagagca tcgtactgct tcagtttcat
     1381 tgattttaca gcagaaacgt ccgaattcca tactccgttg taggctccgc agtcagagag
     1441 agtattgaac tggccacagt ttggtttcag tcccatcatt cctttgagcc cgtttcctac
     1501 agccacaatg aaaagtgcct cttcaaaagt gagagatgcg tccagttttg ccttggctgg
     1561 a
//
LOCUS       DAOQGJ010001143         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60064_length_1560_cov_2.878405, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001143 DAOQGJ010000000
VERSION     DAOQGJ010001143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60064_length_1560_cov_2.878405"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PK683_15855"
     CDS             complement(<1..1248)
                     /locus_tag="PK683_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09971.1"
                     /translation="MKKSAVLLCFFFFMTVHFPSSARPGGGSSYRGSSSSSRPSSSSS
                     SRSSSSSSYKSSSSSSSYRSSSSTTTGSSYHRTISNPSTPSHSSVSTPKPPNSAQSAY
                     LANDSEYEVRQFRVSMKLNRDTGIEIRESMTLDLSGIKSGIVFRTLENSAFSTETEKK
                     LSDKIADTDLKYSIGSFTGTELKLEKSDEHSDFKKIGSGLVSKFYALKRTGPVNLKNE
                     QVSLSRKSYGHIRNEKGQAYLLYPLSPLSGKTSLLKAVKAEVELPPGFETGDIQIIDS
                     GTMQPALTAGKSVLRVGNKLSLEVNMPSLSGPLLLKTAVPQDSVDSSLIPEEVYAESV
                     NIIVPESEIRILLGKDGVFYVTDSFQAKRANKNSSGSVIYRMAEEEPLVLNLPKHFQK
                     QTFFDAEYSPKDLSYYSSSYYKSL"
BASE COUNT          447 a          334 c          336 g          443 t
ORIGIN      
        1 aagggattta taataggaag aggaataata actcagatct ttcggactgt actccgcatc
       61 aaaaaaagtc tgtttctgaa aatgcttcgg aagattcagc acaagcggct cttcctctgc
      121 catcctgtaa atgactgaac cggaagagtt cttattggcc ctcttcgcct gaaaagaatc
      181 agtcacataa aacacaccgt cttttccgag aagaatcctg atctcagact caggaacaat
      241 aatatttacc gattctgcat agacttcttc aggaatcaga gagctgtcga cagagtcctg
      301 cggaactgca gtcttcagaa gcagaggacc gcttaatgac ggcatattca cttctaatga
      361 taatttattc ccaacccgca gaacgctctt ccctgcagtc agtgcaggct gcatagttcc
      421 tgaatcaatg atctgaatgt ccccggtctc aaaaccggga ggaagctcca cttctgcctt
      481 gactgccttc agcagagaag tttttcccga cagaggactc aaagggtaaa gaaggtatgc
      541 ctgacccttc tcattcctga tatgtccgta tgattttctg gacagagaga cctgttcatt
      601 tttcagattt acaggaccag ttcttttcag agcataaaac ttggagacaa gaccagaacc
      661 tattttttta aagtcactgt gctcatctga cttctcaagc ttcagttccg ttcccgtgaa
      721 tgaaccgatg gaatacttca gatccgtatc ggcaatttta tccgaaagtt ttttttccgt
      781 ctcagtggaa aaggctgagt tttcaagagt tctgaaaact ataccggatt ttattccgga
      841 gagatctaaa gtcatagatt ccctgatttc aattcctgtg tctctgttca gtttcataga
      901 tactctgaac tgtctgactt catattcaga atcgttcgcc agataggctg actgtgcaga
      961 atttggaggc ttaggagtcg aaacagagct atgacttgga gtggaaggat tggaaattgt
     1021 cctatgatag gaacttcctg tcgttgtgct ggaactgctt ctgtaggaag aactggaaga
     1081 agaactttta taggaagatg aggaggaact tctggaggaa gaggaactgg atggcctgct
     1141 cgaagatgag gaacccctgt aactgcttcc gccccccggc cttgcagaag aaggaaaatg
     1201 aaccgtcata aagaaaaaaa agcagagcag aacagcagac tttttcataa gaacctctca
     1261 agtttagacg tttccgttga tgattaaatt tcgattctga tcaaattcag taaaatccgg
     1321 aatccggaaa aaaaggactt tatataagag actgtccatt ttttctggac tcagcatgtc
     1381 cctgctaatc ataaatctgg ggatgttgat ctccctcatc tgtttttata caggtttttt
     1441 ttaccgtaag aaaaacagta cgatccataa aaatttaggc ctgaccggcc tcggtatttc
     1501 attgctttcg gctttgtatc ttctggcagg caaatacctg ggcggaggaa tcagttctat
//
LOCUS       DAOQGJ010001144         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60079_length_1560_cov_2.604651, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001144 DAOQGJ010000000
VERSION     DAOQGJ010001144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60079_length_1560_cov_2.604651"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK683_15860"
     CDS             complement(<1..354)
                     /locus_tag="PK683_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09972.1"
                     /translation="MKVILAVFFLIILNIGSQETGGKVYIGEFLPFNSEVKSGLEGRI
                     QAVLESYLAKQGYSTEKISDVSPTKVKDKVPQTAHLYIAGYYTKKEGKNLSIFAQIYH
                     PGTGDMIDAVNIEDEW"
     gene            664..1377
                     /locus_tag="PK683_15865"
     CDS             664..1377
                     /locus_tag="PK683_15865"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HNH09973.1"
                     /translation="MLIGIIFALLFIPAHINSEECTSEESFAVLELFTSQGCASCPSA
                     DSLLKKVYNPNKKIYAISLHVDYWDYLGWKDPYARKENSEKQKEYFRKMSWRVYTPQI
                     VMNGQSHFIGSDEEKIMQNLSVKLPNRNDSRLNLSLSRGQNELKVNFKTICTSSNSVL
                     KVHLLRKNVKNKVSNGENAGLLLEHINVSEGYQEFDLGKGGSGLAKFNLKTSPSEYRI
                     LAYIQNRNSNLILAANETE"
BASE COUNT          482 a          317 c          307 g          454 t
ORIGIN      
        1 ccattcatcc tcaatattga ctgcatctat catatccccc gttccaggat ggtaaatctg
       61 tgcaaatatg gaaagattct tcccttcttt ctttgtataa tatccggcaa tataaagatg
      121 tgcagtttga ggaaccttgt ctttgacttt agtcgggctt acatccgaga ttttttctgt
      181 ggagtagccc tgcttcgcaa gataagactc aagaacagct tgaatccgtc cttccaggcc
      241 tgatttcact tccgaattga acggaagaaa ttctccaatg taaaccttgc cccctgtctc
      301 ctgggaccca atattcagaa taattaaaaa aaataccgca agaattactt tcatcttctg
      361 ctcctgtttt tccccttccc tgatttaaac gaccgaactg tggcgaagag tcattattag
      421 tcgaacactg tttacggaaa gctgaaactt ctctcatttt gagatgtttt tttgtttttc
      481 catttttttt tataatgata atctctttta tcagagttta atgattgact gaaagtacag
      541 aatgaaactt gtcagcggcc ggacagcatc actgtctcat ttgaaatgaa aaggaagttt
      601 tccggcagcg taaatgcctg ttctgctgct caagattccg taaaggagga tatgttgaaa
      661 aaaatgttaa ttggaataat ttttgcactt ctcttcatac ctgcacatat taattcagag
      721 gaatgcacct cggaagaatc atttgccgtc ctggaacttt ttacttcaca gggatgtgca
      781 agctgtcctt ctgctgattc acttttgaaa aaagtctaca atccgaacaa aaagatatat
      841 gccatatcgc ttcatgtaga ttactgggat tacctcggat ggaaagaccc ctatgcaagg
      901 aaagaaaaca gtgaaaaaca gaaggaatat ttcaggaaaa tgagctggag agtatatact
      961 cctcagatcg ttatgaacgg acagtctcat ttcatcggct cagatgaaga gaagattatg
     1021 cagaacctga gtgtgaaact gccgaacaga aatgattcga gactcaatct ttctctttcc
     1081 aggggacaga atgaattgaa ggtaaacttc aagactatat gcacctctag caactcggtg
     1141 ctgaaggtac atcttctcag aaaaaatgtc aaaaacaagg tgagcaacgg agaaaatgcc
     1201 ggactgcttc tcgaacatat caatgtatcg gagggatatc aggagtttga tttaggaaag
     1261 ggcgggtcag gacttgccaa attcaacctg aaaacttctc cttcggaata cagaatactg
     1321 gcttacatcc agaacagaaa ctccaatctt atacttgccg ccaatgaaac agaataaaat
     1381 tctcattaca ggaaaagacg ggatgcttgc tcagagtttt cagggcattc tgaataaaaa
     1441 agggtttgaa tttaaggcat actcaaggag cgaactcaat atccgtatca gagatcatat
     1501 ccggcgcctt cctggatatc tatgatcttc ttctggaaat atgtgccaaa agagtttttc
//
LOCUS       DAOQGJ010001145         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60080_length_1560_cov_2.583389, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001145 DAOQGJ010000000
VERSION     DAOQGJ010001145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60080_length_1560_cov_2.583389"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PK683_15870"
     CDS             <1..476
                     /locus_tag="PK683_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09974.1"
                     /translation="LLRRGTGIFIIDCAIRFNVFRVADFFEEIGEDPEFYLHRIIVQR
                     AFTPYQILDSAVQAENRKNENLIHIFLAPFKQFFDGDVKKDEAGFLLKKLIIKYSVIK
                     NKGIPIVIAEKENYPSSLFSEAMSELEKCADMEWQFSAPRNSPDELSEKQSLFQT"
     gene            491..781
                     /locus_tag="PK683_15875"
     CDS             491..781
                     /locus_tag="PK683_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09975.1"
                     /translation="MGRTISAYSGQMDNVMKRLNNFRRGLRKEDQEAFDFLMRAARLQ
                     VQSGVMASNPNPLDSMILSALVSLKRENLQLKKDLDLIKESLGKFQNERPSV"
     gene            762..>1560
                     /locus_tag="PK683_15880"
     CDS             762..>1560
                     /locus_tag="PK683_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNH09976.1"
                     /translation="MKGRLFDIYHIEEKIYLWVMDSEGKMHVFQDCHYPVIYLHGRQD
                     IMDRIVARLRDLNALSEEPSWEIRKHFYSNQECRALRLSVSKPSILKKMHKKLYVFYG
                     IFDIYHSDIDLLSHYMNFRNIFPLALLEIESEGDRIIRIKSLEDIHSLEYEIPRMKIM
                     EMKLSQSHRLPISLKNHIVFKSGYNIYKIYNNDPKKMISEINAVMEIEDPDVILSSFG
                     DQKILPVLFSISQDLKIPLLLDRDTKMKTVRTILTKGTSYNTYGQWIY"
BASE COUNT          522 a          273 c          330 g          435 t
ORIGIN      
        1 aacttctcag aagaggcaca ggcatattca tcatagactg tgcgatcaga ttcaatgtat
       61 tcagggttgc tgattttttt gaagaaatcg gcgaagatcc tgaattttat ctgcacagga
      121 ttatcgtgca gagagcattt acgccatacc agattctgga ttctgccgta caggcggaaa
      181 acagaaaaaa tgaaaatctg atccatatat ttctggctcc gttcaaacag ttttttgacg
      241 gtgatgtaaa aaaagacgaa gcaggatttc ttctgaagaa gctgattatt aaatattctg
      301 taattaaaaa taaaggaatt ccgattgtaa ttgcggagaa ggaaaactat ccttcttctc
      361 ttttcagtga agcaatgtcc gaactggaaa aatgtgcaga tatggaatgg cagttttccg
      421 cgccgcgaaa ttctccggat gaactctcag aaaaacagtc tctgtttcag acataaaaga
      481 gggataagaa atgggaagaa caataagcgc ttacagcggt cagatggaca atgtaatgaa
      541 gagactgaat aatttcagaa gaggtctcag gaaagaagat caggaagctt ttgactttct
      601 gatgagggca gccagactgc aggtgcagtc aggagtcatg gcatcaaacc ccaatcccct
      661 ggactcaatg attctttctg cacttgtgag tctgaaaaga gaaaaccttc agctgaaaaa
      721 ggatctggat ttaataaaag aatctctcgg gaaatttcag aatgaaaggc cgtctgtttg
      781 atatctatca tatagaagaa aagatttatc tctgggtgat ggacagtgaa gggaagatgc
      841 atgtttttca ggactgtcat tatcctgtaa tttatcttca tggcagacag gatataatgg
      901 acagaattgt cgcaagactg cgggacttga atgccctttc cgaagaacca tcctgggaaa
      961 tacggaaaca tttttattca aatcaggaat gcagagcgct cagactgagc gtttcaaagc
     1021 catccatact taagaaaatg cataaaaagc tttatgtatt ctacggaata ttcgatatct
     1081 atcacagcga tattgatctg ctgtcgcatt atatgaattt caggaatatt tttcctcttg
     1141 ctctcctgga aatcgaatcg gaaggagaca gaatcattcg gataaagtct ctggaagata
     1201 ttcattccct tgaatatgaa attcccagaa tgaagattat ggaaatgaaa ctctctcaga
     1261 gtcatcgcct tccgatttcc ttgaaaaatc atattgtttt taaatcagga tataatatat
     1321 acaaaatata taataatgat ccgaagaaga tgatttcgga aatcaatgcg gtaatggaaa
     1381 tagaagaccc tgatgtgatt ctttcttcct tcggcgatca gaagattctt ccggtcctgt
     1441 tcagcatatc ccaggatttg aaaattccgc ttctgctgga cagagataca aaaatgaaaa
     1501 cagtaaggac tattctgaca aaaggtacaa gttataatac ttatggacag tggatataca
//
LOCUS       DAOQGJ010001146         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60339_length_1556_cov_2.975350, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001146 DAOQGJ010000000
VERSION     DAOQGJ010001146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60339_length_1556_cov_2.975350"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="PK683_15885"
     CDS             <1..1387
                     /locus_tag="PK683_15885"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNH09977.1"
                     /translation="FISSAASWTYIHKRIKEFHISLLFLELGVLGVFASANLVLFYVF
                     WELMLIPMALLIGIWGGETRVYAAVKFFIYTMAGSVLMLAGILIIYFKTGTVSIEELS
                     IANIDNFSPELQTFLFFAFALSFAIKIPVFPLHTWLPDAHTEAPTAGSVILAGVLLKM
                     GTYGFIRFCIPFFPTVSLHYKEVLMVLSVIGIVYGALVAMVQKDAKKLIAYSSVSHLG
                     FCTLGLLTFTEEGVAGGMIQMINHGVSTGMLFLLIGMMYERTHTRMIADYGGIAKIVP
                     LFSVFFMIAMLSSVGLPGTNGFVGEFLVLLGALKANVILGIIAGTGVIWAACYLLWLT
                     KRFLFGELTNKENELLSDLNAREIAILIPMTILVFWLGIYPKSFMVYLEPSIRAYLNS
                     ASVQAISERTIYNGTQDMKPAPKNYTKSYKTLGAPVLSFEERLGKNAGHFALKTGSQP
                     AAPKAEEKHGH"
BASE COUNT          385 a          328 c          341 g          502 t
ORIGIN      
        1 cttcatttcc agtgcggctt cctggactta tatacataaa agaatcaaag agtttcatat
       61 ctctcttctt ttcctggaac tgggcgttct aggagtattt gcatctgcaa atctggttct
      121 cttctatgta ttctgggaac tgatgctgat tccaatggct cttctgatcg gtatttgggg
      181 aggcgaaaca agagtatatg cagcagtgaa attcttcata tatacaatgg ctggatctgt
      241 tctgatgctt gccggtatac tgattatcta tttcaagact ggaactgttt ccatcgaaga
      301 actttccatt gccaatatag acaatttttc accggaactg cagacatttc tgttctttgc
      361 atttgcattg agttttgcca tcaagatccc cgttttccct ctgcatacgt ggcttccgga
      421 tgcgcacact gaagctccta ctgccggttc tgtcatcctc gcaggggttc ttctcaaaat
      481 gggaacttac ggatttatcc gtttctgcat tcctttcttt cctacagtat cactgcatta
      541 taaagaagtt ttaatggttc tgagcgtgat aggcattgta tacggagctc ttgttgcaat
      601 ggttcagaaa gatgcaaaaa aactgattgc atacagctct gtatcgcatt taggtttctg
      661 cactcttgga cttctgactt ttacggaaga aggagttgca ggcgggatga tccagatgat
      721 caatcacggg gtctctactg gaatgctctt ccttctgatc ggtatgatgt atgaaagaac
      781 tcatacaagg atgattgcgg attacggcgg aattgcaaag attgtccctc ttttctctgt
      841 attctttatg atcgcaatgc tctcatctgt gggacttcct ggaacaaatg gatttgttgg
      901 tgagttcctg gttctgcttg gtgcattgaa agcaaacgta attttgggta tcattgctgg
      961 aactggagta atctgggctg cgtgttatct tctctggctc acaaagagat tcctgtttgg
     1021 ggaactgaca aacaaagaga atgaacttct ttctgatctg aatgctcggg agattgcaat
     1081 tctcatcccg atgacaatcc ttgtattctg gctcgggatc tatccgaaat cttttatggt
     1141 ttatctggaa cccagcatca gggcttatct gaattctgcc tctgttcagg cgatttccga
     1201 gagaacgatt tacaacggaa cgcaggatat gaaacctgct ccgaagaatt atacaaaaag
     1261 ctacaagaca cttggagctc cggttctttc ttttgaagaa agacttggaa aaaatgccgg
     1321 tcattttgct ctgaagaccg gttctcagcc tgcagcaccg aaggctgaag aaaaacacgg
     1381 acattgatga ggaaataaaa tgaatcttac accggaaaca aaagaccttc tctccatttt
     1441 tcctgctctt cttctgggcg ggggaggtgc gatattttta gtgctccaat tcttatttcc
     1501 agcaaatgat cagaggattc tccgtttttc ttccgggctt cttcttttaa tttcgc
//
LOCUS       DAOQGJ010001147         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60348_length_1556_cov_2.646236, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001147 DAOQGJ010000000
VERSION     DAOQGJ010001147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60348_length_1556_cov_2.646236"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1556
                     /locus_tag="PK683_15890"
     CDS             <1..>1556
                     /locus_tag="PK683_15890"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH09978.1"
                     /translation="DRVYKVVDKASAFVTRNIIAAPLVANDEVIGVMEAINTIDRNNF
                     NASDISLFLSFSGAAALAIQKTRLLEDLEITNLNLEKRVSELAGLFELGLAVAESQTV
                     TDLLKKSLHIIARELKSDITAIFLEDPAKRFIECIALRNSRLENSRLISDSQSLIVKS
                     IAENRLINESSAVLKSGVDREFLSGAFLILPINDGAGKPFGAISITSHTMKTHYDQAM
                     IRLLLTISSQMMKGYENIKLNENMLAKKAMEKEIEITRNIQTNILPGKLPTGSNFEIG
                     VKSVPAKEVSGDFYDFHKYDDGQFSFLIADVSGKSLPAAIFMAMSSSIIRTLSRAGNV
                     PPSDLLKEANTLIYEDSQSGMFVTLFYIHYDPADYEISFASAGHNDQIWIKNDGTYEL
                     VKGSGAPLGVVPAMKYTGGKFTPANGDLIVLYTDGAVEEKNRAEEEYGLDRFVSEIIK
                     RKDKHPSQIIEEVYQDIKNFSEGQDQFDDFTVLILKINNDFQFRKSFPAANIQIPKLR
                     DFIESVLVTR"
BASE COUNT          495 a          322 c          349 g          390 t
ORIGIN      
        1 atgacagggt ctataaagtg gtggacaagg catcagcatt tgtcactaga aacatcattg
       61 ccgctccttt ggtggcaaac gacgaggtca tcggggtaat ggaagcaatc aatacgattg
      121 acaggaacaa tttcaatgcc agcgatatca gcctcttcct gagtttttca ggagcagcag
      181 cgcttgcgat tcagaaaaca agactgctag aagatctgga aatcacaaat ctcaacctgg
      241 aaaaaagagt gagtgaactg gcgggtctct tcgaactggg actggcagta gcagaatcac
      301 agactgtgac tgatcttctg aagaaatccc ttcatatcat agcacgtgaa ctcaaatcgg
      361 acattactgc aatatttctg gaagatcctg cgaagcgttt tattgaatgc atcgctctca
      421 gaaacagcag gctcgaaaac tccagactga tctctgacag tcagtcgctg attgtaaaat
      481 ccattgcaga gaacaggctg atcaatgaat cttcagcagt tctgaaaagc ggggtggaca
      541 gggaattcct ttcaggcgca ttcctgattc tgccgatcaa tgacggtgca ggaaaacctt
      601 tcggtgcaat ttccataacg tctcatacaa tgaagacaca ttatgatcag gcaatgatcc
      661 gtcttctcct gacaatctct tcacagatga tgaagggata tgaaaacatt aaattaaatg
      721 aaaatatgct cgcaaagaag gcaatggaaa aggagataga aatcacaaga aatattcaga
      781 cgaatattct tccgggaaag ctccctaccg gttccaactt cgagatagga gtcaagtcag
      841 taccggcaaa agaagtctca ggtgattttt acgattttca taaatatgat gacggacagt
      901 tttcatttct gattgcagac gtatccggaa aaagtctgcc tgcagccatc tttatggcta
      961 tgagctcctc catcatccgc actttaagcc gtgcaggaaa tgtgcctccg agcgatcttc
     1021 tgaaggaagc aaacacactg atctatgagg actctcagtc agggatgttt gtgactcttt
     1081 tttacataca ctatgatcct gcagattacg aaatcagctt tgcatccgca ggacacaatg
     1141 atcagatctg gatcaagaat gacggaacct atgagcttgt aaaaggaagc ggagctcctt
     1201 taggtgtagt acctgcaatg aaatatactg gcggtaaatt tacccctgca aacggagacc
     1261 tgattgttct ttacactgac ggtgccgtgg aggaaaaaaa cagggcagaa gaagaatacg
     1321 gactggatag gtttgtatct gagatcataa aaagaaagga caaacatccg tctcagatca
     1381 tagaagaagt ttatcaggac ataaaaaact tttcagaagg acaggaccag tttgatgatt
     1441 tcactgtgct cattttgaag atcaacaatg atttccagtt ccgaaaatct tttccggcag
     1501 caaatatcca gattccgaaa ctgagagatt ttatagaaag tgtcctcgtt accagg
//
LOCUS       DAOQGJ010001148         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60395_length_1555_cov_3.308000, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001148 DAOQGJ010000000
VERSION     DAOQGJ010001148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60395_length_1555_cov_3.308000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PK683_15895"
     CDS             complement(<1..1177)
                     /locus_tag="PK683_15895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH09979.1"
                     /translation="MRCNIFEKNKLSFCMLIGNLPNNVIRYDRLCRAIYASPSMEKVN
                     GKGASEKLLGKTPTEAVTETVYDVSEYQSKIEYVIATGETVEMELKAPQPTGEVQIHH
                     IRIIPEFSENGEISGALAVGGDITERKAAEEMIHLLTHSINASSEQFFLIRTDYPSFI
                     YVNDKAAKTLGYTKEELTSGKGVFDIDPDVNNMELWKDHVKRLEKEGSRVFETRHRSK
                     NGKVYPVEIRSNFFTHNGGRYILSIANDITEKKKMEKALMSHKQELQSLAESSPGIMG
                     SFHLKADGSICMPYVSPNISEILGLNQQDVMKDAEIMILWSHPEDRERIRQTIEESAR
                     SMTVWHEEYRILHPEKGIRWMESNTKPEPHPEGGVVWYGYVHDITERKLVEQKIEYLA
                     "
BASE COUNT          359 a          361 c          287 g          548 t
ORIGIN      
        1 aagcaaggta ttctattttt tgttctacca gcttgcgttc agtgatatca tgtacatatc
       61 cgtaccagac aactcctcct tcaggatgcg gttcaggctt tgtattgctt tccatccagc
      121 gaatgccttt ctccggatgc aatatcctgt attcctcatg ccatacagtc atactgcgtg
      181 ccgattcttc aattgtctgc ctgattcttt ccctatcctc cggatgactc cataaaatca
      241 ttatttctgc atctttcatt acatcctgct gattcagccc cagaatctcg gaaatattcg
      301 gagatacgta aggcatacag atggatccgt ctgctttcaa atggaagctt cccattatac
      361 ccggcgaaga ttcagcaaga gactgaagtt cctgcttgtg cgacatcagt gctttttcca
      421 tttttttctt ctctgtgata tcgttggcga tggaaagaat gtagcgtccc ccattgtgag
      481 taaagaaatt tgatctgatt tctaccgggt aaacttttcc atttttcgac ctgtgtctag
      541 tctcaaaaac tctgcttcct tccttttcca gcctttttac atgatccttc cataactcca
      601 tattgttgac gtccgggtct atatcaaaaa ccccttttcc tgaggtaagc tcctcttttg
      661 tatatccgag tgtcttagct gctttgtcat ttacatatat aaaactggga taatctgttc
      721 tgatcaggaa aaactgttcg gatgatgcat ttatggaatg cgtgagcaga tgaatcattt
      781 cttccgctgc ttttctttcc gtaatgtcac ctccaactgc aagtgctccg cttatctcgc
      841 cgttttcaga aaattcagga atgatgcgga tatgatgtat ctgaacttct cccgtcggct
      901 gcggagcttt cagttccatt tcgactgttt cgcccgttgc aattacatat tcgattttcg
      961 actggtactc acttacatca tagactgtct cagttaccgc ttctgtcggt gttttaccca
     1021 gcagtttctc tgaggcgcct ttgccgttta ctttttccat tgacgggctt gcatagatgg
     1081 ctctgcaaag acggtcatac ctgataacat tgttaggcag gtttccgatc agcatacaga
     1141 aactcagttt gtttttttca aatatattgc atctcatttt tttccttttg ttcagtcggt
     1201 cgtctttttt atccctgcct tcgggagcgt tctctggttt tttctaatat aattaaaata
     1261 tattattatt caatgcagac cgaaatccta ttgccctgat ttccagtcaa gcatagcgag
     1321 attggaaatc agggatgaat gtggagtcct gccgaagggg tattgcacct tcttgggaat
     1381 cccccctttt ttattcacat tcagtgataa gaccaattga ttttcagtca atgctgtatt
     1441 gatcctatta taatctaatt agaaatccgg gtattgacca tcctctctga agaaacgaac
     1501 tgtattcaat ttgttctttg atgcatacag aagcagtgcc tagtcttcat tgtct
//
LOCUS       DAOQGJ010001149         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60400_length_1555_cov_3.194000, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001149 DAOQGJ010000000
VERSION     DAOQGJ010001149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60400_length_1555_cov_3.194000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK683_15900"
     CDS             <1..514
                     /locus_tag="PK683_15900"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09980.1"
                     /translation="NVPDVRVEKSSETYFHPNCAVRFFSGQTAAAEAGILHPALYEQY
                     DFKKGIPVIGRIFFDELLPLFTANRNVFQFRTPSSFPQDSFDISLLLDENMGTEAFAE
                     KVKSAGIEEVQEIYVLSIYRGENIPSGKKSVTYRIPLLTFRETFTQARIKEIKDSLFA
                     ISSELNLNIR"
     gene            520..>1555
                     /locus_tag="PK683_15905"
     CDS             520..>1555
                     /locus_tag="PK683_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HNH09981.1"
                     /translation="MGYYLRGSVKSEDVLKKASAGERISADEALILYKEGDFLEIQKV
                     AREKREKILSHKFASYTMFRVVNYTNFCNVECSFCSFMDEIGSGKGYVLSKEEILEKM
                     EYAEREGADQMFLQGGVYPDLKFDYYLDVLSSVKQRFPHFHIRAFSPVEIINLEKITG
                     KPLKDVLKILKDAGLDSVPGAGAEILTERMSNIISPKNASVSEWVRAMETCHEAGLPG
                     SANIVFGSEETEEEVVEHLKVIRDLQDRTKGFHAFIPWTFQPQTKRFKIRSVPTHEYL
                     KVLGICRIFLDNIPHIETSVMVLGKGVGQLALYSGADDVSSVVIEENVLRSFGLKTEA
                     EALKFLSSGGF"
BASE COUNT          451 a          290 c          382 g          432 t
ORIGIN      
        1 gaatgtgccg gatgtaaggg tggaaaaatc gtcagagaca tattttcatc ccaactgcgc
       61 agtcagattc ttttccggac agaccgcggc tgcagaggct ggaattcttc atcctgcact
      121 ctatgagcag tacgatttta aaaagggaat acccgtcatt ggaagaattt tctttgatga
      181 acttcttccc ctttttactg caaacagaaa cgtgtttcag ttcagaactc cttcaagctt
      241 tcctcaggac agttttgata tttctcttct gctcgatgag aatatgggaa cagaggcttt
      301 tgctgagaag gtgaagagtg ccggaataga agaggttcag gagatttatg tgctttcgat
      361 atacagagga gagaatattc cttctggaaa aaaatccgtt acgtacagga ttcccctcct
      421 tacattcagg gaaacattta ctcaggcaag aatcaaagag atcaaagaca gtcttttcgc
      481 gatttcttct gaactcaatc tgaatatcag gtaatgatta tgggatatta tttaagaggc
      541 tccgtcaagt ctgaagatgt tctgaaaaag gcatctgcag gagaacgaat aagtgcggat
      601 gaagcactga tcctttataa ggaaggtgat tttctggaaa tacagaaagt cgcacgcgag
      661 aaaagagaaa agattctttc tcataaattt gccagctaca caatgttccg tgtagtgaac
      721 tatacgaatt tctgcaatgt ggaatgcagc ttctgttcct ttatggacga gataggttcg
      781 ggtaaaggct atgttctcag taaagaggaa atccttgaaa aaatggaata tgcagaaagg
      841 gaaggggcgg atcagatgtt ccttcaggga ggcgtttatc ctgatcttaa gtttgattat
      901 tatctagatg tattatcatc agtaaaacaa aggtttcctc attttcacat ccgggctttt
      961 tctccggtgg aaatcatcaa tctggaaaaa atcacaggga aaccgttgaa ggatgtactg
     1021 aagattctga aagatgcagg gctggactcc gttccagggg ctggagccga gatactgact
     1081 gaaagaatga gcaatattat ctcacctaaa aatgcttctg tcagtgaatg ggtcagagcc
     1141 atggaaacct gtcatgaggc gggtctgccc ggcagtgcaa acatagtatt cggatcggaa
     1201 gagacagaag aagaggtcgt tgagcatctg aaggtaatca gggatcttca ggacagaacg
     1261 aaaggtttcc atgcatttat tccctggaca ttccagcctc agacaaaacg tttcaagata
     1321 cgttccgtac ctactcatga gtatctgaag gtgcttggga tatgcagaat ttttctggat
     1381 aatattcctc atattgaaac atctgtgatg gttctgggga agggagtcgg tcagcttgca
     1441 ctgtacagcg gtgctgatga tgtgtcatcc gttgtcattg aggagaatgt ccttcggtcc
     1501 ttcggtctga agacggaggc ggaggctctc aaatttcttt cctccggagg attta
//
LOCUS       DAOQGJ010001150         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60420_length_1555_cov_2.581333, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001150 DAOQGJ010000000
VERSION     DAOQGJ010001150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60420_length_1555_cov_2.581333"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PK683_15910"
     CDS             complement(<1..1307)
                     /locus_tag="PK683_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09982.1"
                     /translation="MMIRFLLLAGISLSAYADTILMGDGTSITQSCLLEQSNSFLIYK
                     DSKGNVKTLERKEVRSVIIDPGTCPDVGLGSGKITVADEAGKETVFNGYKSGGQEPKG
                     EKKDTNGVNIEIMHEFVTDFIWRGNSYGGEVRARRGGTSYKEISQYYAYQPNIKVAAP
                     SGLYFELWGNIPLMGRGDRDSDHRFQSSPGGAAVNPVALVDRLSNNQVVSADQSSLFF
                     DPSNNVFNSTCSDPAAALATLQTTPGSRTDCQVDLRKVKRHKERNGLYRTDGLFTTFA
                     YNFDAGKFGTFTMGTWWYFQNDKQARYSWQEYFIWWDLPFLKKYLSPKLQVYTQSSQD
                     LSAYADGSNYASFQTKHTFTVKESNGWKFQIEPNNNIGYKYANDNSNRKSGFFDITTG
                     LKFILNDFFLTVNDAYRPDVHLYDNSVFYYDTSNPGRALPNLN"
BASE COUNT          428 a          354 c          332 g          441 t
ORIGIN      
        1 tgattcagat tcggcaatgc ccggccagga ttggaggtat cataataaaa caccgagttg
       61 tcgtacagat gaacatccgg tctgtatgca tcgttcacag ttaaaaagaa atcatttagg
      121 ataaatttca gacctgtcgt aatatcaaaa aaaccggatt tcctgtttga attgtcgttg
      181 gcatacttat agccgatatt gttgttcggt tcgatctgaa acttccatcc attcgattct
      241 tttacagtga atgtatgttt ggtctgaaaa gaggcatagt ttgaaccgtc ggcataggca
      301 ctgagatcct gagaagactg agtatacacc tggagtttcg gggagaggta ctttttcaga
      361 aatgggagat cccaccagat aaaatattcc tgccaggaat accttgcctg tttgtcgttc
      421 tgaaaatacc accaagttcc catagtgaat gtaccgaatt tgcctgcatc gaaattatag
      481 gcaaatgtag taaacagacc gtctgttctg taaagaccgt ttctttcctt gtgtcttttt
      541 accttccgca gatccacctg acagtcagtc cttgaaccgg gggttgtctg cagcgtggca
      601 agtgcagcag cagggtcaga acaggtagaa ttgaatacat tgttggacgg atcgaagaaa
      661 agagaagact gatctgcact gacaacctga ttattgctca gcctgtctac tagcgcaacc
      721 ggatttacag cagcacctcc aggtgaagat tgaaaacgat gatcggaatc tctgtctcct
      781 cttcccataa gcggaatatt tccccaaagt tcaaaataca gaccgctcgg agcggcgacc
      841 ttgatattcg gctgataggc atagtactga gagatttcct tgtaacttgt accaccccgc
      901 cttgcacgga cctcaccgcc gtaactgttc cctcgccaaa tgaagtctgt gacaaactca
      961 tgcataattt cgatattgac tccattcgtg tcttttttct cacccttagg ttcttgtccg
     1021 ccggacttgt atccgttgaa gacagtttct ttccctgcct catccgcgac ggtaattttg
     1081 ccgcttccca gtcccacatc aggacaggtt ccaggatcaa tgatgaccga ccgaacttcc
     1141 tttctttcaa gggttttaac attacctttc gaatccttgt agatcaggaa ggagttggac
     1201 tgctcgagaa gacagctttg agtaatggaa gtcccgtctc ccataagaat cgtgtctgca
     1261 tatgcagata aggaaattcc tgccagaaga agaaatcgaa tcatcatttt ttaccttcca
     1321 taaaaatctc tatcgtttat atttgcccgc attccgacat cagttttccg acggaaatac
     1381 agaaacctgc cctgcaggcc gactgaaaaa ttaactgaaa aagtggaatt tcagacattt
     1441 tatccgaaag gagttttcga tcaaggataa tttcggaaag tgcctggcag agacagaaac
     1501 tgctgtcctt ttaaaaccga gacttcagga ttgaagtaat ttttctgatt ccggt
//
LOCUS       DAOQGJ010001151         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60476_length_1554_cov_2.973316, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001151 DAOQGJ010000000
VERSION     DAOQGJ010001151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60476_length_1554_cov_2.973316"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..606
                     /locus_tag="PK683_15915"
     CDS             34..606
                     /locus_tag="PK683_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09983.1"
                     /translation="MELVKRLHLNGGAGNTNTQYEKGTVSNGKLGFEYRPIPYIGLGM
                     GIAGSNLAVKEKQSSADLLLPLLLLSGSSSSSSSSSFLTTIILLDMLSSGGTSKLSYN
                     TLNLDFNFHMNQDKTFDPYIGIGVIGGSCTGAATCTVTGGEGRLGVQFNFSTFFFFLQ
                     GQYQSLSFKAADSGVNATNTIGSLGLGARF"
     gene            complement(681..>1554)
                     /locus_tag="PK683_15920"
     CDS             complement(681..>1554)
                     /locus_tag="PK683_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNH09984.1"
                     /translation="VSSGIDSSTIVPNAKPGQCFSKVIIPADYKEVVEQYIKKPASER
                     TETVPPQYELIQEKILVRPATVRYEEIPAVYEVVEEKVLVKPASKKVIELPPVYETVE
                     ERVVDKEGYTVWKKSEDSNLLCLIEVPPVYKTIQKQVLKVPASKTEEVVPEEYSIIKR
                     QIEKIPPSTRKVDVPAEYQTVAVKKLIEPEKQIRIPVPAEFENITRTVMVREAATEWR
                     RVLCENNATPSKISKIQNALKTSGFDPGRTDGLADTNTFNALNSYQKSKGLPQDPDKY
                     INLESVKSLGVETD"
BASE COUNT          388 a          324 c          327 g          515 t
ORIGIN      
        1 acagtgcaga aagctttttt ttgattgtat ggaatggagt tagttaagcg gttacattta
       61 aacggaggag cagggaatac aaatactcaa tatgaaaaag gaaccgtatc caacggaaag
      121 ttaggattcg aatacagacc gattccctac attggtttgg gaatggggat agccgggtcg
      181 aatctggcag taaaagaaaa gcagagctca gcagatttac tcttgccatt actccttctc
      241 tcgggatcct cttcatcctc ctcttcaagc agttttctga ctactattat tcttctggat
      301 atgctttcat ccggcggaac ttcaaaactg agttacaata cattgaatct ggattttaat
      361 tttcatatga atcaggacaa gacatttgat ccttatatcg gtataggggt tattggaggc
      421 tcctgtactg gcgcagcaac atgtactgtg actgggggag aaggaagact tggagtgcag
      481 ttcaatttca gtacattctt tttcttcctt cagggacagt accagtcact cagcttcaag
      541 gctgctgaca gcggagtaaa tgcaacaaac acaatcggaa gtttaggact tggtgctcga
      601 ttctgatcta tcggagttgc tgtgcagatg ctgcctttaa aggcagcatc tgcgacaatc
      661 gacacatcga tggtgttttt ttaatctgtt tcaactccca gtgatttgac agattccaga
      721 ttgatgtatt tatccggatc ctgaggaagt ccttttgact tctggtaact gttgagtgca
      781 ttgaatgtat ttgtatctgc caacccgtca gttctgcctg gatcaaatcc ggaagtttta
      841 agcgcattct gaattttgga aattttggaa ggagttgcat tgttttcgca aagaactctt
      901 ctccactcag ttgctgcttc tctgaccatt acagttcttg taatattctc aaattctgcc
      961 ggtacaggaa ttctgatctg tttttcaggc tctatcagtt ttttcactgc tacagtctgg
     1021 tattctgcag gaacatccac ttttctggta ctcggaggta ttttttcaat ctgtcttttt
     1081 ataatactgt attcctcggg aacaacttcc tctgtcttgg aagctgggac tttcaatacc
     1141 tgcttctgaa ttgtcttata aactggagga acttcaatca ggcagagaag attgctgtct
     1201 tcactttttt tccatacagt atagccttcc ttatcaacga ctctttcctc aacggtttca
     1261 taaaccgggg gaagttctat gacttttttg gaagcaggct tcactagcac cttttcttcg
     1321 acaacttcat agacagcagg tatttcttca tatctgacgg ttgcaggccg gaccaatatc
     1381 ttttcctgaa tcagttcata ctgcggagga acagtttcag ttctttccga tgccggtttt
     1441 tttatgtact gttccactac ttctttataa tcagcgggaa ttataacttt gctgaagcat
     1501 tgtcccggtt tagcattagg cacaattgta gagctgtcga ttccgctgct cact
//
LOCUS       DAOQGJ010001152         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60498_length_1554_cov_2.320881, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001152 DAOQGJ010000000
VERSION     DAOQGJ010001152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60498_length_1554_cov_2.320881"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..681)
                     /locus_tag="PK683_15925"
     CDS             complement(253..681)
                     /locus_tag="PK683_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase/dehydrase and lipid
                     transport"
                     /protein_id="HNH09985.1"
                     /translation="MIETSVEGLARKPLSAVFSYIWNLESMTEYNSSVKHSRRISDSD
                     TGLPVFEISVGFELFQLKNLYKVESFEKDRFFSASCESSAVYFKDFYEFTEQADGTYI
                     RISDRMELKGLLKLAEPVVRNNMKHQMQENMNRLLQILNQ"
     gene            810..1229
                     /locus_tag="PK683_15930"
     CDS             810..1229
                     /locus_tag="PK683_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09986.1"
                     /translation="MPEPLKNMFDEKFVSGLAEDIRKVYREFDSANFIRDVFDGKWQE
                     KELKERMRHITECLKKHLPGPYEEAVSILVKVSSEHRTKRKLNDEISFCDMVFPDFTE
                     KYGIDHYDISVKSMEEFTKVSSSEFAVRIFIIKYPKL"
BASE COUNT          499 a          265 c          301 g          489 t
ORIGIN      
        1 gtaatcttcc ggaagacgga atccaagctg tttttcaaac tcaagtatat ccggttcaga
       61 tgcgccatcc tcgagcgagg aatacaggta ttctgaattt ttttccaaaa aatgttctat
      121 gtctttccag atttcgcgga cagtcataat gcaaggatta gggatttgag ctctgtgtca
      181 agtgagggat ttttcaaatt tgcgaaccct cacgcttagg ctgccttagg ctgcagcagg
      241 cgtatgatga gtttactgat tcagaatctg cagaagtctg ttcatatttt cctgcatctg
      301 atgtttcata ttgtttctga cgactggttc cgccagtttc aaaagacctt tcagctccat
      361 cctgtctgaa atacggatat aggttccgtc tgcctgttca gtaaactcat aaaaatcctt
      421 aaagtaaaca gcggaacttt cgcaggaagc cgaaaaaaat ctgtccttct caaaactctc
      481 cactttgtac agattcttca gttgaaatag ctcaaaccct acactgattt caaatacagg
      541 cagtcccgtg tcagaatcac tgatcctcct ggaatgtttt acgctcgaat tgtattcggt
      601 cattgactcc agattccaga tatacgaaaa tacagcagac aaaggctttc ttgccagtcc
      661 ttctacagag gtttcaatca taatatttcc ctttaaaatg agaatcgttt taaatcgaga
      721 agaattcggt gtttttatca gaatattcaa ttttactaaa gaattgacag atctgtctat
      781 ggatgaaaca taagcgcctg agagatgata tgccggaacc gctgaaaaat atgtttgatg
      841 aaaagttcgt ttccggactg gctgaagata ttcggaaagt ttaccgggaa tttgattctg
      901 cgaatttcat cagagatgtg tttgatggga aatggcagga aaaagaactg aaggaaagaa
      961 tgaggcatat cactgaatgc ctgaaaaaac atcttcctgg cccttacgaa gaggccgttt
     1021 ccatccttgt aaaagtgtct tccgaacaca ggacaaaaag aaaactcaat gatgagatca
     1081 gtttctgtga tatggtcttt cctgacttta cggaaaaata cggaattgat cattatgata
     1141 tatctgtaaa gtcaatggaa gaattcacca aagtttccag ttctgaattt gcagtaagaa
     1201 tttttatcat aaaatatcct aaattgtaaa atattatcgt taaaaggaat cgatatatta
     1261 gtttggttac tagcaagtaa gggatattta agagacccaa taatttgaag tcctatttta
     1321 gaattgtatt ttaccttttt caagatatat aataagagtt taagtgaggg gggaggtaaa
     1381 gctgatctga aaaggagcaa tagcaaaata aatctcggat attgaagtat cattctgaat
     1441 ggtttttgat tttatcatat cacaataatt tatttaagat gcagcttata gtttgttata
     1501 atgaatggaa ttaataataa caattaattg tatagaatac ttaatcgaat attt
//
LOCUS       DAOQGJ010001153         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60693_length_1551_cov_2.931818, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001153 DAOQGJ010000000
VERSION     DAOQGJ010001153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60693_length_1551_cov_2.931818"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="PK683_15935"
     CDS             <1..1306
                     /locus_tag="PK683_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH09987.1"
                     /translation="AIKDPATFVSYFLMINLAGLRLDRRFSVFTGALTVLSFTALLLM
                     GLASGTVSFTSDPSLAYDPKYLRASVELSKILFLIAASAIIVYLSGDTRRFLSQLSVS
                     ETRTQHNLKVMGEILQHTEEVSVRLRDLMKGLVIQADSVQASVNDQKIFFEKDLDTVK
                     SMMQESREINLVINSQLGMLEKISSRVDSLLSVADQVKKESTESVSKAVQVKKIITEG
                     QAFLENAMSSVQDMKAQSEKILNISRTINEIAESTSLLALNASIEAARAGVNGRGFAV
                     VASEVQKLADRSINSSKEIHTIINATVKNIDRSSSMILETSKQLEVIAEAVKENEVFL
                     GGLSENAVKQGKLSFAIKSDVENIREIGESVLALTVGQDENLSAFSERNRKKLEMTAK
                     SEELVNRLEEITKEMGVYSSKLYDLVKERDRLISEDKRMSQN"
BASE COUNT          481 a          301 c          379 g          390 t
ORIGIN      
        1 tgccatcaag gacccggcaa cctttgtatc ttatttttta atgatcaatc ttgcaggact
       61 cagactggac agaaggtttt ccgtatttac cggagcactg actgttctca gttttactgc
      121 tcttcttctg atgggacttg cgagcgggac tgtctcattt acatccgatc cttctcttgc
      181 ctatgatccg aagtatctga gggcttctgt agaactttcc aagatacttt ttctgattgc
      241 ggcatctgcc attatagtgt atctttccgg agatacaaga cgtttcctga gccagctttc
      301 tgtttctgaa acaaggactc agcacaatct taaagtgatg ggggagattc ttcagcacac
      361 agaggaggtt tctgtccgtc tgcgtgatct gatgaaagga ctggtcattc aggcggattc
      421 cgtgcaggca tcagtgaatg atcagaaaat cttttttgaa aaggatctgg atactgtgaa
      481 gagcatgatg caggaaagca gagagatcaa tcttgtcatc aattcacagc tggggatgct
      541 tgaaaaaata tcctcacgcg tggactctct tctttccgtt gcagatcagg tgaaaaagga
      601 aagcactgaa tctgtcagca aggcggttca ggtcaagaag atcattacgg aaggacaggc
      661 tttcctggag aatgcaatgt cttccgttca ggacatgaaa gcacagtctg aaaagatact
      721 gaatatttcc agaacaatca atgagattgc ggaaagcaca agtctccttg cactgaatgc
      781 ttcaattgaa gcagcaagag caggagtgaa cggaagaggt tttgctgtcg tggcatccga
      841 ggtgcagaaa ctggcagaca ggagcatcaa ttcttccaag gaaattcata caatcatcaa
      901 tgcgactgtg aaaaatattg acagatcatc ttccatgatt ctggaaacat cgaagcagct
      961 ggaagtgatt gcagaagcag tcaaagagaa tgaagtgttt ctcggcggac tttccgagaa
     1021 tgcggtgaaa cagggcaagc tcagttttgc aatcaaatcg gatgtggaga atattcgaga
     1081 gatcggtgaa agtgttctcg cactcactgt cggtcaggat gaaaatcttt ccgcattttc
     1141 cgaaagaaac agaaagaagc tggagatgac agcaaaaagc gaggagctgg tcaaccgcct
     1201 cgaagagatt acaaaagaaa tgggggtcta ctcttccaaa ctttatgatc tggtcaaaga
     1261 aagagacaga ctgatcagcg aggacaaaag gatgagccaa aactgatatg tgtaagtatt
     1321 attactcaga attggaaata gacagtaaga gaatttatag aaaatagctc gaaaaagcgg
     1381 ggtcttagca gcttcaagct ggaagcaata ttgaagagaa ggatataaat aaatatgaaa
     1441 ggaacagaat tctcgataag ggacagactg aaaagtttta tctatgcgtt caacgggatc
     1501 aggcttctga tccgcactga gcacaacgcc agaattcatc tgaccgcagc c
//
LOCUS       DAOQGJ010001154         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60696_length_1551_cov_2.783422, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001154 DAOQGJ010000000
VERSION     DAOQGJ010001154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60696_length_1551_cov_2.783422"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..733
                     /locus_tag="PK683_15940"
     CDS             38..733
                     /locus_tag="PK683_15940"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNH09988.1"
                     /translation="MNEFAVRSGKHSYCLTESAGGHRIVLELEPESEMQISLNERSAA
                     ISLLKGKLKIDSEKIQNDSVLTARTEDLTIEFLGTKLFLRRDSGVSALTVLKGSVKAV
                     QSESKDRIISENRSEKFNHSRKEWTSEKMSDSETAKLKKEFDELIPVSPNELRNGNYG
                     NQSANIQYSRRVSEKMNSQKISQISGRKVRLKNGKTLDAETIFQEEDRYIIITKDDIF
                     HMKDTEVDAILFQ"
     gene            759..1520
                     /locus_tag="PK683_15945"
     CDS             759..1520
                     /locus_tag="PK683_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09989.1"
                     /translation="MFVSGISPDRVKLKNGKIHDNVKTSLRGNTLSIQHENGKQEMIP
                     ASEIAELKPSKVQWKQTASPKTAESEISAKEEKKEIAQAEPETLSEKQSLSSKGSYNY
                     LYSLIPGWSSLNTGRYWYAGIGISLLELGALRKISGRNELRPLLDDEDAVNLHIISAL
                     NMKETGTGHPLRDAALIGYYRRHTLDGRDATDSTESFLNRRNGFIALSVITVIDVVLA
                     GALQEKAEQKADSAFSFTFRTAGKENFSLAFTKNF"
BASE COUNT          546 a          300 c          331 g          374 t
ORIGIN      
        1 actgcagtat cacgagctgt tagttgcctg agatcagttg aatgaatttg cagtaagatc
       61 cggaaagcat tcttactgtc tgactgagag tgcaggcggc cacaggattg tactggaact
      121 tgagcctgaa tcagagatgc agatttctct gaacgaaaga tcagcagcaa tcagtcttct
      181 gaaaggaaaa ctgaaaatcg attccgaaaa aatacagaat gattcagttt tgactgcaag
      241 aacagaagat cttacaattg aattcctcgg aacaaaactt tttctccgca gagattcagg
      301 agtatctgca ctgactgtac tgaaaggatc agtaaaggca gttcagtcgg aaagcaaaga
      361 cagaatcatc tccgaaaaca gatcagaaaa attcaatcat tccagaaagg aatggacatc
      421 cgaaaaaatg tccgactcgg aaactgcaaa actgaaaaaa gaatttgatg aactgattcc
      481 agtttctccg aatgagctaa ggaacggaaa ctatggaaat cagtcagcaa acattcagta
      541 ttccagacga gtatcagaaa aaatgaattc tcaaaaaatc tctcagattt caggcagaaa
      601 agtccgtctg aaaaacggga agacacttga tgcagaaaca atatttcagg aagaagacag
      661 atatataatc attacaaaag atgatatttt ccatatgaag gatacagaag tagatgcaat
      721 actttttcag taaggcggtt tttattgcag cagcagttat gttcgtatca ggaatttctc
      781 cggacagagt aaaactgaag aatggaaaaa tccatgataa tgtaaaaaca tctcttcgag
      841 gaaacacgct ttctatccag cacgaaaacg gaaaacagga aatgattcct gcatcagaaa
      901 ttgcagaact caaaccttcc aaagtccaat ggaaacagac tgcatcccca aaaacggctg
      961 aatctgaaat ctcagcaaag gaagaaaaaa aagaaatcgc tcaggctgaa ccagaaacgc
     1021 tttcggaaaa acagtcttta tcttctaaag gcagttacaa ttatctctac agtctgattc
     1081 caggctggtc ttctctgaac acaggcaggt attggtatgc aggaatagga atcagcctgc
     1141 ttgaacttgg cgctctaagg aaaatctcgg gcagaaacga acttagaccg ctattggatg
     1201 acgaagatgc agtcaatctc catatcatat ctgctttaaa tatgaaagaa acaggtacgg
     1261 gacatccatt gagagatgct gcgctgatcg gatattaccg aagacatacc ttggacgggc
     1321 gggacgcgac tgacagtaca gaatcctttc tgaacagaag gaatgggttt attgctcttt
     1381 cagtcataac tgtaattgat gtagttcttg ccggagcgct tcaggaaaaa gcagagcaga
     1441 aagcagactc ggcattcagc tttacttttc gaacagccgg aaaggaaaat ttttccctgg
     1501 cgttcacaaa aaatttttag atccctgtct gccgtcagat attattttgg t
//
LOCUS       DAOQGJ010001155         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60716_length_1551_cov_2.186497, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001155 DAOQGJ010000000
VERSION     DAOQGJ010001155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60716_length_1551_cov_2.186497"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK683_15950"
     CDS             <1..332
                     /locus_tag="PK683_15950"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNH09990.1"
                     /translation="EIEILIPVIEKYRKRGWNIEGPVPADSIFTKEQIIKYDIILCCY
                     HDQGLIPFKHIAGKNGVNTTLGLDFIRVSPDHGTAFDIAGKGIADPASFKACLKLIEN
                     RNTPEKN"
     gene            complement(676..1173)
                     /locus_tag="PK683_15955"
     CDS             complement(676..1173)
                     /locus_tag="PK683_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09991.1"
                     /translation="MVKKIVKALFNSILLIVCVSNCNSLEERQKNAIETVLREDKSLM
                     VAAQNENKNKKNQIAVFTVYTQQMKMISLEHCPPDFSESFRKHIYAWEDAVKQMRKKD
                     DDFLGSLFGGLIGFFTGNAPLALGSLAKILADNGVDMQQVTDTWREVELSAIRYKAKL
                     PGNQD"
     gene            complement(1269..>1551)
                     /locus_tag="PK683_15960"
     CDS             complement(1269..>1551)
                     /locus_tag="PK683_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09992.1"
                     /translation="FQNITGCKFQDIYARASNGEHFIYYKFEENCKFTKYLMPPGYQQ
                     GGPEEPTLDTKGTLIMLKNVILAKKSTNGDITGIFFINKNNGISYNMLY"
BASE COUNT          480 a          330 c          252 g          489 t
ORIGIN      
        1 aggaaatcga aatactgatt ccagtcatcg aaaagtatcg aaaaagaggc tggaatattg
       61 aaggacctgt ccctgctgat tccattttca caaaagaaca gataataaaa tatgatatta
      121 tactttgctg ctatcacgat cagggactga ttcctttcaa gcatattgca gggaaaaacg
      181 gagtcaacac cactctcggc ctggacttta tccgagtttc tcctgatcat ggtactgctt
      241 tcgatattgc aggaaaagga atcgctgatc cggcaagttt caaggcctgc ctgaaactga
      301 ttgagaacag aaacacacct gaaaaaaact gattctaaaa ttcagaagaa gatttttgcc
      361 gactataaac actttttagg aagttcgcaa aaacccatca gtggaaaaag aagcccatcc
      421 atatgcgtac agacatctgt tccgtctttc acaaaagaaa tcccgtattt ttccggtgca
      481 tttcggacag ctgattcaca gatcaggata gatctcaatt gacagttcct ttttttcttt
      541 ctttcagcat cacaattgtt cagctttttt tgtacttcac agaaactgaa aagcaataag
      601 aaaattacgg acaatagtgt ataaagacag gatcggtaat gaatcatttt aaatctcagt
      661 tttcttgact tccctttaat cttgattgcc gggaagttta gccttatacc tgatagccga
      721 taattcaact tctctccagg tgtctgttac ctgttgcata tcaacaccat tatcagctaa
      781 aatctttgca agagatccta aagccaatgg ggcgttccca gtaaagaatc ctattaatcc
      841 tccgaaaagg cttcccaaaa agtcatcatc ttttttcctc atctgcttca ctgcatcttc
      901 ccatgcgtaa atgtgcttac gaaaagattc cgaaaaatct ggtggacaat gctctaaact
      961 gatcattttc atctgctgag tatacacagt aaatacagca atctgatttt ttttattttt
     1021 gttttcattt tgagctgcaa ccattaaact cttatcttcc cgtaaaactg tctctatagc
     1081 atttttctgc ctttcttcca gagaattgca gttagaaaca caaacgataa gcaaaattga
     1141 attgaacaat gccttgacta tctttttcac caaaaccttt actccttcat cacagcagag
     1201 atatcctcaa aattctattg gctctaaaaa accttaatat aagaccactt agttgtacgc
     1261 atttccgatc agtaaagcat gttgtaactg attccattgt tcttgtttat aaagaatatc
     1321 cctgttatat ctccgttggt agattttttt gccaggatta catttttgag cataattaaa
     1381 gtgcctttag tatcaagggt aggctcctca ggtcccccct gctgataccc aggcggcatc
     1441 aaatattttg taaacttgca gttctcctca aatttatagt aaataaaatg ctcaccgttt
     1501 gaagcccgag cataaatatc ttgaaatttg catccagtta tattctgaaa t
//
LOCUS       DAOQGJ010001156         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60743_length_1550_cov_3.877592, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001156 DAOQGJ010000000
VERSION     DAOQGJ010001156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60743_length_1550_cov_3.877592"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PK683_15965"
     CDS             <1..1473
                     /locus_tag="PK683_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09993.1"
                     /translation="KFWAAWCGPSHEYLGTMTDLQKKHENRITFAALSAEETEDVEPF
                     LEKKKKKIEYSVGCAPARLYQAYLNGIKGIPHCFIVDEEGLLVWHGHPSELEKTLELI
                     FDTERSLGDLIKAFIKKEKFLELFNEIKNGSLEKLNRKSKMKNLAREYLEMFPDDQDA
                     LEILENYPILLPGEKYADLGEISWLKDSAEIGTGYSLIDFWDLENGESLNTFPHISAV
                     HKKFKNKITVIGLTKNSAPSEEISEFLERHTDDISYPVGAVSEEVYKICLPDEDAWQG
                     CTALLNSENVLLWSGKAAHTHFIISHLLEKNGTEKDILDHCSDRAMFLQMDEEFSADY
                     DPVSEQSFDKLLKLGERVLSINPADTEILTILSIYAKDLGYEAMQSVCGLADISYLNN
                     EQVLSLLSDLQMGSKDVHPFEFILRSLESVLKTDPSVSNILYYGDILKSLHKLDMALE
                     YYEKALECNEQTEDIERKKAEILNMKNAADFAKKISQILP"
BASE COUNT          503 a          291 c          351 g          405 t
ORIGIN      
        1 aaattctggg cagcctggtg cggtccgagc catgagtacc tcggcacaat gactgatctg
       61 cagaaaaaac acgaaaacag aatcacattt gctgcactga gtgcagaaga aacagaagat
      121 gtagaaccgt ttctggagaa gaaaaagaaa aaaatagaat acagtgtggg ctgtgctcct
      181 gcaagactct atcaggcgta tctgaacgga atcaagggaa ttcctcactg ctttatagta
      241 gatgaagaag ggcttctcgt ctggcatgga catccttccg aactggaaaa aactctggaa
      301 cttattttcg atacagaaag atccctgggt gatttaataa aagcatttat taaaaaggaa
      361 aaatttcttg aactcttcaa tgaaataaaa aacggcagtc ttgaaaaact gaaccgcaag
      421 tcaaaaatga aaaacctcgc acgtgaatat ctggaaatgt ttccggatga tcaggatgca
      481 ctggaaattc tggaaaacta tccgatcctt ctgcccggag aaaaatatgc tgatctgggc
      541 gaaatcagct ggctgaagga ttcagctgaa atcggaaccg ggtattctct gattgatttt
      601 tgggatctcg aaaacggaga atctctgaat acttttccgc atatctcggc agttcataaa
      661 aaatttaaaa ataaaattac agtcattgga cttacgaaaa attctgcacc gtctgaagaa
      721 atcagtgaat ttctggaacg tcatacggat gatatcagtt acccagtcgg tgcagtctct
      781 gaagaagtct acaagatctg tcttcccgat gaagacgcat ggcagggctg cacggcgctt
      841 ttaaattcgg aaaatgtgct gctctggtcg ggaaaagcgg ctcatactca tttcattata
      901 tctcatcttc tggaaaaaaa cggaactgaa aaagacatat tggatcactg ctccgacaga
      961 gcgatgtttc ttcagatgga tgaagagttt tcggctgatt atgatcctgt ttcagagcag
     1021 agtttcgata aacttctgaa gcttggagaa agagtcctga gcataaatcc tgcagatacg
     1081 gagattttaa ccattctctc tatttatgca aaagacctgg gttatgaggc aatgcagtca
     1141 gtatgcggtt tagcggatat atcatatctg aataatgaac aggtgctttc tctcctctct
     1201 gacctgcaga tgggcagtaa agacgtgcat ccctttgaat tcatactccg cagtcttgaa
     1261 agtgttctga agaccgatcc gagtgtaagc aatatattgt attacgggga tattctgaaa
     1321 tccctgcata agctggatat ggcactggaa tattatgaaa aggcactgga atgcaatgag
     1381 cagacagaag atattgaaag gaaaaaagca gagattctga atatgaaaaa cgcagcggat
     1441 ttcgcaaaaa agatttctca gattctgcct taaagacagg cttgaaacct ttctttactg
     1501 accgggattc agatgcaggc ggccggaaaa gtaagtaatt ttatttattg
//
LOCUS       DAOQGJ010001157         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60786_length_1550_cov_2.294983, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001157 DAOQGJ010000000
VERSION     DAOQGJ010001157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60786_length_1550_cov_2.294983"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PK683_15970"
     CDS             complement(<1..408)
                     /locus_tag="PK683_15970"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNH09994.1"
                     /translation="MQNAVLDAGPLIALFDNSDKYHSQVLDFFKTYKGILYTSWPVIT
                     ETMHFLNFNPEVQSDFLIWLQRDILQIPFLDKSALENIRRLILKYKNVPMDLADASLL
                     HISEEQNLRYVITLDSDFIIYRVKKKGFKNLIHI"
     gene            complement(389..598)
                     /locus_tag="PK683_15975"
     CDS             complement(389..598)
                     /locus_tag="PK683_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNH09995.1"
                     /translation="MEEISRLEKKSKSEVLRHALEAYLEAYNAKTSFYESGKEFFGKY
                     GSGVSDNSVNYKKKLKESIRAKRSS"
     gene            complement(681..1304)
                     /gene="mtnB"
                     /locus_tag="PK683_15980"
     CDS             complement(681..1304)
                     /gene="mtnB"
                     /locus_tag="PK683_15980"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HNH09996.1"
                     /translation="MKSELEFIVQFSKTYYSRQWMAATAGNLSVISEDRNSFFVTASG
                     KDKGSLTPEDFLEVALSDGKPVGAAGLKPSAETSIHTAVYSSLKSVGAILHVHTVASC
                     IPVFGLTRSRNSTYVPLPNTEILKAFGDFTEEPNFKMLVIHNFASVPEISKILSTELQ
                     LKDQDVPFFLIENHGLTVWGKDVKDANKNLEAAEFVMQVFAARNRLF"
     gene            complement(1349..>1550)
                     /locus_tag="PK683_15985"
     CDS             complement(1349..>1550)
                     /locus_tag="PK683_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNH09997.1"
                     /translation="GVIPPNFAILQVTYAEEGLKGDTSGTALKRVKVETGGEINVPIF
                     VKQGDVVKIDLRDLTYVERVNK"
BASE COUNT          421 a          330 c          338 g          461 t
ORIGIN      
        1 tatatgaatc agatttttaa atcctttttt ctttactctg tatatgatga agtcggaatc
       61 aagagtgatc acataacgca ggttctgttc ctcagagata tggagaaggc ttgcatccgc
      121 cagatccatc ggaacatttt tgtatttcag gataagtctc ctgatattct ccaaagcaga
      181 tttatccaga aaagggatct gcagaatgtc tctctgcagc cagatcagga aatcggactg
      241 gacttccgga ttaaaattca ggaaatgcat cgtttctgta attacaggcc agcttgtata
      301 aagaataccc ttgtacgttt tgaaaaaatc cagaacctga gagtggtatt tgtcggaatt
      361 gtcaaagagc gcgatcagag gaccggcatc aagaactgcg ttttgcacga atggactctt
      421 tcagcttttt cttgtagttg actgagttgt cagaaactcc gcttccgtat tttccgaaaa
      481 attcttttcc tgattcgtaa aatgaggttt ttgcattgta cgcctcaaga taggcctcca
      541 gggcatgacg cagcacttcc gatttggatt tcttttccag tctggaaatc tcctccaatc
      601 tggactctag gattctaggc agtctgagtg caaacatgta ttacatgatg tcatacatag
      661 agaaattgtc aagaaaattt tcagaaaagc ctgtttcttg cggcaaatac ctgcattaca
      721 aattccgcag cttccagatt tttgttcgcg tccttcacat cttttcccca gacagtcagg
      781 ccatgatttt caatcagaaa gaaggggaca tcctggtcct tgagctgaag ttcagtggaa
      841 aggattttgg agatttccgg aacagaagca aaattgtgaa tgacgagcat tttaaaattc
      901 ggttcctctg taaagtctcc gaatgctttt agaatttcag tattcggaag agggacatag
      961 gtggagtttc tggatctggt cagaccgaag acggggatac aggaggcaac tgtatgcaca
     1021 tgcagtattg cgcctactga ttttaatgag gaatagacag cagtgtggat ggatgtttct
     1081 gcacttggct tcagcccagc tgcaccgaca ggctttccgt cactgagtgc aacttccaga
     1141 aaatcttcag gtgtgagtga acccttgtct tttccggatg cggttacaaa aaaagaattt
     1201 ctgtcttcac ttattacaga aagattgccg gcagtagctg ccatccactg ccgactgtaa
     1261 taagtctttg aaaactgaac tataaactcc agctcagatt tcaatgttat gctccggcac
     1321 ctgcagaatg aggaaagcat cagtcatgct atttgttgac tctttccacg taagtcagat
     1381 cccgaagatc gatttttaca acgtcgccct gtttgacgaa tataggcacg ttgatttccc
     1441 cgcctgtttc cacttttact cttttcagtg cagttcctga agtgtctcct ttgagacctt
     1501 cttcagcata agtcacctgc agaatggcaa agttaggcgg aatgacaccg
//
LOCUS       DAOQGJ010001158         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_60925_length_1548_cov_2.924983, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001158 DAOQGJ010000000
VERSION     DAOQGJ010001158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60925_length_1548_cov_2.924983"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PK683_15990"
     CDS             complement(<1..659)
                     /locus_tag="PK683_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09998.1"
                     /translation="MNFKPDKAKFKKRIIIMHSVTVSLMIVLIAFSSFNASNNDFRLP
                     VILCAMTLILFLVFRGNLKRQLKILEESVIRLEAQTMVLENSSGVCETVPLDEVEKIY
                     LDSMLGQTKLILVGKTDWARTYIDIENMDSFTEGLEKAVGIKSEKFPRNWKEIGIKTG
                     LIFIPSFITLIFASVYKFNMNMNIFFLVFTLNSLFCVYNLSEKKTFGGIPASTSRRMI
                     I"
     gene            complement(661..>1548)
                     /locus_tag="PK683_15995"
     CDS             complement(661..>1548)
                     /locus_tag="PK683_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH09999.1"
                     /translation="HSRSSMKKITIQIVFIFLSFGHSLYAGESFRLIFSHSLKGNVNA
                     CTCTAIPIAGLLKRDFFFQKIQVSPDRDILIEAGDFYAPSQDAKKKPALEEGFSRMGY
                     HLLVPGMNEFKNAGSDELNRFSHLTVSNIKITGSSSQFTSGIGILERNGRKVVILPYM
                     TKAAFLRIPEPYRKKYSHIPFDAVYRYVQENVKHDFLILAVYGKLADAPEWISVKKEK
                     SVVILGGDETKSSSGFSDTASGRIYHNSGKNGDDISVLEISLKDFKVIQSKVTTLDAD
                     KEKDSERIQKILDKYGIKD"
BASE COUNT          446 a          337 c          292 g          473 t
ORIGIN      
        1 ataatgatca ttcgtctgga tgtcgatgca gggattcctc cgaatgtctt cttttcagaa
       61 aggttgtaaa cacagaaaag agagtttaaa gtaaacacta gaaagaatat attcatattc
      121 atattaaatt tatataccga tgcaaatatc agtgtgatga aacttgggat aaagatcaga
      181 ccagtcttga tcccgatctc cttccagttg cgaggaaatt tttccgattt aattccgacc
      241 gctttttcca atccttccgt aaaggagtcc atattctcga tatcgatata ggtccttgcc
      301 cagtcggttt taccgacaag gatgagcttt gtctgtccga gcatactgtc cagatagatt
      361 ttctccacct catcgagggg aactgtttcg cagacaccgg aagagttttc caaaaccata
      421 gtctgtgctt ccagacggat gacagattcc tccaggattt tcagctgcct tttcagattt
      481 ccgcgaaaca caaggaaaag aatgagtgtc attgcacaaa gtatgactgg cagacggaaa
      541 tcattatttg aagcattgaa tgagctgaaa gcaatcagta cgatcatcag actgactgtg
      601 actgagtgca taatgatgat gcgttttttg aactttgcct tgtccggttt gaaattcata
      661 tcagtcctta attccgtact tgtctaaaat cttctgaatt cgttctgaat ccttttcttt
      721 gtccgcatcc agagttgtca cttttgactg aatcacttta aaatccttca gagaaatttc
      781 cagaacagat atatcatctc cattctttcc tgaattatga tatattctgc ccgaagccgt
      841 atcagaaaaa ccggaggaag attttgtttc atctcctccc agtatgacaa ccgacttttc
      901 cttctttacc gaaatccact caggtgcgtc agcaagtttt ccatacactg ccagaatcag
      961 aaaatcatgc tttacatttt cctgcacata tctgtacacg gcatcaaacg gaatatgaga
     1021 atactttttt ctgtaaggct cagggattct caaaaatgct gcttttgtca tataaggaag
     1081 gattactacc tttctgccgt tcctttctaa aattccaatg ccggaagtaa actgggacga
     1141 tgaaccggta atctttatat ttgagacagt caggtgtgaa aaccgattca gttcatccga
     1201 acctgcattc ttgaactcat tcattccggg aaccagaaga tgatacccca tcctgctgaa
     1261 gccttcttcc agggccggtt tcttttttgc atcctgtgac ggtgcataaa aatctcccgc
     1321 ctcaattaag atgtccctgt ccggggaaac ctggattttc tggaaaaaga aatctctctt
     1381 cagaagtcct gcaatcggaa ttgcagtgca tgtacacgca tttacatttc ccttgagact
     1441 gtgggaaaag atcagacgga agctttcacc cgcataaaga gaatgtccga atgaaagaaa
     1501 aataaagact atttgaattg taattttttt catactactc cttgaatg
//
LOCUS       DAOQGJ010001159         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61012_length_1547_cov_2.282172, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001159 DAOQGJ010000000
VERSION     DAOQGJ010001159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61012_length_1547_cov_2.282172"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK683_16000"
     CDS             <1..190
                     /locus_tag="PK683_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10000.1"
                     /translation="GRGFHIILNDKSDETAQSAKFKIREVLRNLGLQADVLETVPASL
                     EDVFVFLIGSLEEKVNAS"
     gene            180..1307
                     /locus_tag="PK683_16005"
     CDS             180..1307
                     /locus_tag="PK683_16005"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH10001.1"
                     /translation="MLRRIYAMAWKESLHIIRDFRTLYIAFVIPIFLILLFGYAVNFD
                     IDHIPVGICDQDSSMQSREFISKIQAGGWFEAKTVTSEPQEIQDALQKGRIRIGLFIP
                     AEFHKKLNRGETAQIGAVLDGSDNGSANAGYNYLQFFFNEYKFSILRKSFQQYGIHPP
                     AVPEIKPRILFNPELKSRYYMLPGIIVLITAIMSAMLTSLTAAKEWERGTMEQLISTP
                     VRPHEIILGKILPYTAISMIQSLISASVAVWIFELPFEGRIADLLITNLFFSIGALGI
                     GITVSSVLKSQLPAIQVSLIASMLPSLFFSNFVFPIDSMPLPLRILSGIVPAKYFLLM
                     LRTIFLKGTGFSAYYTEFIFLTVFCFLVLAAASRNTPKRIL"
     gene            1304..>1547
                     /locus_tag="PK683_16010"
     CDS             1304..>1547
                     /locus_tag="PK683_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10002.1"
                     /translation="MTTALRALIRKELIQTLRDKKLRVVLFVLPVVQLIIFGTAISTD
                     VKKTKVSLCDEDRSKASREIHSILTAGDYFEIADIYG"
BASE COUNT          449 a          323 c          332 g          443 t
ORIGIN      
        1 aggaagaggt tttcatataa tactgaatga caagtcagat gagacagctc aatccgcaaa
       61 gttcaaaata cgggaagttt tgcgaaatct aggcctgcag gccgacgtac tggaaactgt
      121 tcccgccagt ctcgaagatg tattcgtatt ccttatagga agcctggagg aaaaagtaaa
      181 tgcttcgtag aatttatgca atggcctgga aagaaagtct gcatattata agagatttta
      241 gaacactgta cattgcgttc gtgattccga tatttctgat tctccttttc ggatatgcag
      301 taaatttcga catagatcat attcctgtcg gaatctgcga tcaggacagt agtatgcaga
      361 gcagagagtt tatttccaaa attcaggcag ggggctggtt tgaagcaaag acagtcacat
      421 ccgaacctca agaaattcag gatgcccttc agaaaggaag gataagaata ggactgttca
      481 ttccggcaga atttcataaa aagctcaaca gaggggaaac tgctcagata ggagctgttc
      541 tggatggaag cgacaacggt tcggcaaatg caggatataa ttatcttcag ttttttttta
      601 atgagtataa attctccatt ctccgaaagt cctttcagca gtatgggata catcctccag
      661 cagtgcctga aatcaagccg agaatacttt tcaatcctga actgaaaagc agatactata
      721 tgcttccagg aatcattgtg ctgatcactg caattatgtc ggctatgctc acttctctta
      781 cagcagcaaa ggaatgggaa agaggaacaa tggagcagct gatttccact cctgtccgtc
      841 cgcatgaaat catactggga aaaatattac cttacaccgc catcagtatg atccaaagtc
      901 tgatctctgc atcggttgct gtctggattt ttgagcttcc gtttgaagga aggattgcag
      961 atctgctgat cacaaatctt ttcttttcca tcggtgcact tggaatagga atcacagtct
     1021 caagcgttct gaaatcacag cttccggcaa tccaagtgtc actgattgct tctatgcttc
     1081 cttcattgtt cttttccaat tttgtattcc caattgattc aatgcccctt cctctcagaa
     1141 tcctttccgg aattgttcct gcaaaatact ttcttctgat gctgagaaca atatttctga
     1201 aggggacagg tttttcggcc tactatacag agttcatttt tctgacagtg ttctgcttcc
     1261 tggtactggc agctgcatcc agaaatacac cgaagaggat tttatgacga ctgcactgcg
     1321 agcactgata agaaaagaac tgattcagac tttgagagac aaaaaactca gagttgttct
     1381 gtttgttctt cctgtcgttc agctcattat attcggcacc gcaatttcga cggatgtaaa
     1441 aaagacaaaa gtgtctctgt gcgacgaaga caggtcaaag gcttccagag aaattcactc
     1501 catcctgact gcgggagact atttcgaaat cgcagacatt tatggag
//
LOCUS       DAOQGJ010001160         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61032_length_1546_cov_3.775989, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001160 DAOQGJ010000000
VERSION     DAOQGJ010001160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61032_length_1546_cov_3.775989"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..690
                     /locus_tag="PK683_16015"
     CDS             298..690
                     /locus_tag="PK683_16015"
                     /inference="COORDINATES: protein motif:HMM:NF018738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HNH10003.1"
                     /translation="MDSLKSMVGAGAPGVEVKLLKEKASVQEPVKGIAVLTGGEYPVT
                     IDKVILQMLMVEEIPGKDKPKETLVKVGTMTMNEFILDPKEKVSLPFQVKIPKDNLIS
                     SSAIKHYVQVTLDVSGQDPFGVCEIQVV"
     gene            complement(693..>1546)
                     /locus_tag="PK683_16020"
     CDS             complement(693..>1546)
                     /locus_tag="PK683_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10004.1"
                     /translation="RLLLPSVFMFLLLFEDRLHEEWFFSEKTVLGLNVLLILSVVYYS
                     SYKGEPRTGSEWLNAFLKRNLPRTEVPFVKGTVADERKAYYGELNQDLKSRFSEIKYK
                     WYTEGTKYRKLQECLNFEPMIISNSLTRAKCADGFGLGYFAVAAGPNVDVADELGFTH
                     KNVLPRTKEEEPRPGHLKVMELAEVIRTGSMFCSLDDKRYDSIFKTKYGIIIRPELKI
                     LASFGSEEYRKKVRKLKRLYSEVSKSRKKEDILLFQRLQLIEKTDRFSILSLPDRISS
                     EKKNCWD"
BASE COUNT          471 a          327 c          332 g          416 t
ORIGIN      
        1 agaacagaat ggatttgaaa tatagaaatc tcaagccttt tttacgtatt cgcgtagagt
       61 gtttggaact gtataaaagc tttaaaaaat gaggttcacg cggaggcgcg gaggcagaaa
      121 aaatggaatc gaaatcaaat ttaatagatc atttttttta caggactgag caaaagtctg
      181 cacaaaagac ttgactgagt ctgaagagaa atgaaaaatc aaagccggta taactgacgc
      241 agtactgaag cgccgggtgc gtcaaatcat ttatgagagg ctgtcaatat gggatttatg
      301 gattctttaa aaagtatggt gggtgccgga gcaccgggtg tagaagtaaa acttctgaaa
      361 gaaaaggcat ctgttcagga accggtcaaa ggcatagcgg tgctcacagg cggtgaatat
      421 ccggtcacaa tcgacaaggt catactgcag atgctgatgg tggaagaaat tccaggcaaa
      481 gacaagccga aagaaacact ggttaaggtc ggcacaatga caatgaatga atttattctc
      541 gacccgaaag aaaaggtctc ccttcccttt caggtaaaaa ttccaaagga caatctcatc
      601 agctccagtg caatcaaaca ctatgtgcag gtgacactgg acgtttccgg tcaggatcca
      661 ttcggtgtct gcgagataca ggtagtctga cttcagtccc agcagttttt cttttcagaa
      721 ctgattctgt cgggaagaga aaggatgctg aatctgtcag tcttctcgat gagctggagt
      781 ctttgaaaaa gcagaatatc ttccttcttt ctgcttttcg atacctccga atacagccgc
      841 ttcagcttcc tcactttctt tctgtattcc tctgaaccga aactggcaag aatcttcagc
      901 tcaggcctga taatgatacc atattttgtt ttaaatatac tgtcgtatct tttatcatcc
      961 agagaacaga acattgatcc tgtgcggata acctcagcca gctccattac tttcagatga
     1021 cccggacggg gctcttcttc ctttgtgcgc ggaaggacat ttttatgagt aaaaccaagc
     1081 tcgtcagcca catccacatt cggacctgca gcgacagcaa aataccccag accgaatccg
     1141 tccgcacatt tggctcgtgt cagtgaattg gaaataatca taggttcaaa attaagacat
     1201 tcctgaagtt tcctgtattt tgttccttct gtataccatt tgtatttgat ttccgaaaag
     1261 cgggatttca gatcctgatt cagttcacca tagtatgctt tcctctcatc agctacagtt
     1321 cctttcacaa agggaacctc tgtccttgga agatttcttt tcagaaatgc attcagccat
     1381 tcacttccag tacggggttc tcctttgtat gaagaataat aaaccacaga tagaatcaga
     1441 agaacattca gtccaagcac agtcttttcg gaaaagaacc attcttcatg aagcctgtct
     1501 tcaaacagaa gaagaaacat aaaaacggaa gggagcagaa gacgga
//
LOCUS       DAOQGJ010001161         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61044_length_1546_cov_3.026828, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001161 DAOQGJ010000000
VERSION     DAOQGJ010001161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61044_length_1546_cov_3.026828"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PK683_16025"
     CDS             complement(<1..741)
                     /locus_tag="PK683_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNH10005.1"
                     /translation="MKIFRKNRRSGAIVILLVMGIGTSAFITANQYAKDRMMEYRIAK
                     SNAEGFRAFLLARAGMQGALGALKKIPEEALYQSGIAFNPPPVPLGGGTVYYKLTPED
                     GKFNINYLVKSYDQQPNLKMLEMLHRLYEQLGLQREKLSPIIDWIDENSEETGGGAEI
                     FYYNGLKPPRKIKNGPLYSLSELTSVKGYDRKTVYESLKPKDYDKNNSKDFLSEEEKL
                     LLTDNDFVLSNNITAYMPYKDTNDERINV"
     gene            complement(738..1379)
                     /locus_tag="PK683_16030"
     CDS             complement(738..1379)
                     /locus_tag="PK683_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004491879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HNH10006.1"
                     /translation="MKSLSRNLRRGVTLIELAVVMAVLGVLFVGIFSAYHTSLKISRA
                     SDPKHGTSRQNIFFALENIRSTFAQIYLIEGQRRLVFIGKNEGVQNQRLDRVVFAANH
                     PNSEETGRPSVREVSFFLRPMKQNSEFYYLIRREDEMVDRDPESGGIEHILLDHVVSF
                     QLKYSQRGDKWVDEWNSRDTKKIPKLIRIEIIALVGDTDMKYEALANPGMFYK"
     gene            complement(1363..>1546)
                     /locus_tag="PK683_16035"
     CDS             complement(1363..>1546)
                     /locus_tag="PK683_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10007.1"
                     /translation="VYGRFIGLFIQERPDTKEKFLPQKFISSQNINTDMIESINSRPV
                     HILQKYESTALDEVIK"
BASE COUNT          395 a          353 c          315 g          483 t
ORIGIN      
        1 cacattgatt ctttcatcgt ttgtatcttt gtaaggcata tatgcagtaa tgttattgga
       61 gagtacaaaa tcattatcgg tcagaagaag tttttcttcc tcacttaaaa agtccttgga
      121 attattcttg tcatagtcct tcggcttcag cgattcataa acggttttcc ggtcataacc
      181 tttcactgag gtcagttctg acagactgta aagaggtccg tttttaattt ttctgggggg
      241 cttgagaccg ttataataaa aaatttcggc cccgcctcct gtctcttcgg agttttcatc
      301 aatccagtca atgataggcg aaagtttttc tctctgcagt cccagctgct catagagcct
      361 gtgaagcatt tcgagcatct tgagattcgg ctgctgatca taggatttca ccagataatt
      421 gatattgaat tttccgtctt caggagtcag cttataatat actgtgcctc ctcccagcgg
      481 aaccggagga ggattgaatg caatgcctga ctgatacagt gcttcttccg gaattttctt
      541 gagtgcaccg agtgctccct gcattccggc acgggcaaga agaaaggctc ggaaaccttc
      601 cgcattggac tttgcaatcc tgtactccat cattctgtcc tttgcatact gatttgcagt
      661 gatgaaggca gatgtgccta ttcccatcac gagcagaatg acaatggcac cggatcttct
      721 gttctttcgg aaaatcttca tttatagaac atcccgggat ttgcaagtgc ttcgtatttc
      781 atatcagtgt cgcccacgag tgcaatgatt tcaattcgga tgagtttcgg aatttttttt
      841 gtatccctgc tgttccattc atccacccat ttatcccctc tctgagagta tttcagctga
      901 aaactcacca catgatcaag cagaatatgc tcgattcctc ccgattcagg atccctgtcc
      961 accatctcat cctcacgtct gatgagataa taaaactcag aattctgctt cataggacgg
     1021 agaaaaaagg acacttctct gacagaaggt ctgcctgtct cttcagagtt gggatgattt
     1081 gcagcaaaga ccacacggtc aagtctctga ttctgaactc cttcattctt gccgatgaac
     1141 accagccttc tctgaccttc tatgagataa atctgtgcaa acgtggaacg gatattttca
     1201 agtgcaaaaa aaatattctg tctggatgta ccgtgcttgg gatctgatgc tctggaaatc
     1261 ttgagagagg tatgatatgc actgaatatg cctacaaaaa gcactccgag gactgccatt
     1321 acaactgcaa gttcaatcag agtgactcct ctgcggagat ttctacttaa tgacttcatc
     1381 cagtgccgta gattcatatt tctgcagaat gtgtacaggt ctgctgttga tgctctcaat
     1441 catatccgta ttgatattct gcgaacttat gaatttctga ggaaggaatt tttcctttgt
     1501 gtcaggtctt tcctgaatga aaagaccgat gaaccttccg tacact
//
LOCUS       DAOQGJ010001162         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61060_length_1546_cov_2.600939, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001162 DAOQGJ010000000
VERSION     DAOQGJ010001162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61060_length_1546_cov_2.600939"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PK683_16040"
     CDS             complement(<1..535)
                     /locus_tag="PK683_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10008.1"
                     /translation="MKKKQITFTVLLTAVLQLTAQEKQMRIGILEFKPSEGVSKRDAI
                     AVGGFVNSCMTKVKGISVINRSDMKSVLNEQRLSQLGVVSGDDLKIGALISANKVLTG
                     TVSKVAGNYYITGSLTDVNSGEINYSDKVKTNTLDNADIIADIFCNKFSSWNSGGSYV
                     NPYAEYSSELKSETMAES"
     gene            complement(550..1509)
                     /locus_tag="PK683_16045"
     CDS             complement(550..1509)
                     /locus_tag="PK683_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10009.1"
                     /translation="MIIFLYSIALLIIFQNCIPISWNYAKLPKTEEKNPFASGLSVCY
                     IREIGYQKESLPKILPISAKEQKSKDSSFNDEYIFSNAKIEDFGLSEASYLQTVQHLQ
                     ERNIKVNTYQKIIVPEILRPHITSAKIRELKSTVEKKPEFFLETLQKILKQYSESCTV
                     KETEKGSIFTGKDCNALMNSYFSIKLFENLVQSVKFPLLEESGKLSKTQCQYSLIFRY
                     EPESKREISKTVWAKIALHAVTFGVFPYWDSDSRILHLLIQKGTSEENLQSIEIEIGW
                     KRAVSIFFLPTFMLLNKRIKLTNTELAERESNFLKEFLSDLSL"
BASE COUNT          416 a          315 c          280 g          535 t
ORIGIN      
        1 gactttcagc catagtctca ctttttaatt cggaagagta ctctgcatag ggatttacat
       61 aggaacctcc gctgttccag ctggaaaatt tattgcagaa tatatccgca attatatccg
      121 cattgtccag agtattggtt ttgactttat ccgaataatt gatctctcct gaatttacat
      181 cagtcagact tcctgtaata taataattgc cggccacctt ggatacggtt ccagtcagaa
      241 ccttattggc agaaatcaaa gctcctattt tcaaatcgtc tcctgataca accccaagct
      301 gtgagagtct ctgttcgttc agaacggatt tcatatcaga gcggtttata actgaaattc
      361 ctttgacctt cgtcatacag ctgtttacaa agcctccgac agcaatggca tctcttttgg
      421 aaactccttc actcggtttg aattccaaga tccctatcct catctgtttt tcctgtgctg
      481 tcagctgcag aactgcggtg agcagtacag taaaagtgat ctgctttttc ttcatagttt
      541 ttccttcctt tataatgata aatccgatag aaattctttt aaaaaattgc tttctctttc
      601 ggcgagttct gtatttgtaa gtttgattct tttgttcagc agcataaacg tcggcagaaa
      661 gaaaatactt acagctcttt tccatccgat ttctatttct atgctttgca gattctcttc
      721 agatgttcct ttttgaatca gaagatgcag aatacgggaa tcactgtccc agtagggaaa
      781 cactccgaaa gtgactgcat ggagtgcgat ttttgcccaa acggttttgc tgatttcccg
      841 ttttgattcc ggctcataac ggaatatcag tgaatactgg cactgtgttt tgctcagttt
      901 accggattct tcgagtaacg ggaattttac ggactgaact aaattttcaa acagttttat
      961 ggaaaaataa gaattcatca gtgcattgca gtcttttccg gtgaagatgc ttcctttttc
     1021 tgtttccttc acagtgcagg attcggaata ctgcttcaaa attttctgga gagtttccaa
     1081 gaagaattca ggcttctttt cgactgtcga ttttaattct cttattttcg ccgaagtaat
     1141 atgaggtctt aaaatttccg gaacgataat tttctgatat gtattgactt tgatattccg
     1201 ctcctgcaga tgctgcactg tctgaagata ggaagcttct gagagaccaa aatcctctat
     1261 ttttgcattt gaaaaaatgt attcatcatt aaaagaagag tctttgcttt tctgttcctt
     1321 tgcagaaatc ggtaatatct tcggaaggga ttctttttga taaccgattt cccgaatata
     1381 acagacagac aacccggaag cgaatgggtt cttctcttct gtctttggaa gttttgcata
     1441 attccagctg atcggaatac agttttggaa tataattaat aatgcaatac tatacaaaaa
     1501 gattatcata tccgtcttct gtcattacaa tcatttcaag ttcacc
//
LOCUS       DAOQGJ010001163         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61061_length_1546_cov_2.558015, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001163 DAOQGJ010000000
VERSION     DAOQGJ010001163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61061_length_1546_cov_2.558015"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..>1546)
                     /locus_tag="PK683_16050"
     CDS             complement(256..>1546)
                     /locus_tag="PK683_16050"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH10010.1"
                     /translation="KLTSGGKETLEALRTFRAKGMDEFLPRVSEILKKRSTSKEIQVA
                     ILDLYESYGFDLDSHLPDSIDDYEWYFQEKFQEEDVFVRLLNSFEKRRDRKFLYTVIP
                     FVTHKSSEIREATYRYLNSIRDDRALPYIMELGNSEEPIYRYYYLEALQIIRDERTSV
                     HVPRMLADLSPALRIGAIQAAENLNLKDKQTQILGMARGDSNYEVRKTATVYARTTNQ
                     RFRTDIFQGGLFDSHREVREVALESVKSFRDSSYAPAISKALEKEKHSYLSSQMLDTL
                     MVLGNHGGGQGIANALLNDLDENVRIKAAFIASKLSAKEILSSLIGSLSNEPSVNVRV
                     EVAKTLGHLKDKKAVPVLFDRLKDKTENVVLRKEVLSSLDKIDEPSVMPYVFDLLEDE
                     SGAFRSQIRDFMRGMLYRHHGSKLKTVAYTSPELQIN"
BASE COUNT          461 a          337 c          299 g          449 t
ORIGIN      
        1 acctctgaag gagagttcca gttatgtgat tgtgcagaaa ttgctgatga aaaagctaaa
       61 aacagaattc caaaatgttt cagataaaaa tgtctcataa gacctcaaaa tcgtacctga
      121 gacaattttt ttaaagaaac ctactctgtt aatcaaaaaa attctgaaaa gggaaaaatt
      181 ctaggttaga atttcgggta aaggagtgaa aagtatgacc ccccatcaat gaaattagat
      241 ggagggactt gaaattcagt taatttgaag ttcaggagaa gtatatgcta cagtcttaag
      301 tttggaaccg tgatgacggt acagcattcc cctcataaag tcacgaatct gagatctgaa
      361 ggcaccagac tcatcttcca gaagatcaaa gacataaggc attacggaag gttcatcgat
      421 tttatccagc gaactcaata cttcctttct cagaacaaca ttttctgttt tgtccttcag
      481 tctgtcaaaa agaacaggaa ccgctttttt atccttcaaa tgtccaaggg tttttgccac
      541 ttccactctt acgttgacag aaggttcatt gctcagtgat ccaatcagag aagacagaat
      601 ttcttttgca cttaatttcg aagcgatgaa tgcagcttta attcttacat tttcatccag
      661 gtcattcagc agagcatttg caatcccctg accgcctcca tgatttccaa gaaccataag
      721 agtatcaagc atctgcgaac ttaaatagga atgtttttcc ttttccaagg cctttgaaat
      781 cgcaggagca tagctggaat ctctgaagga cttcacactc tccagtgcaa cttcacgcac
      841 ttctctatgg gaatcaaaaa gtccgccctg aaaaatatct gttctgaaac gctgattcgt
      901 ggttctggca taaacagtag cagtcttgcg aacctcataa ttgctgtcgc ctctggccat
      961 acccaaaatc tgagtctgct tgtctttcag attcagattt tctgcggcct gaatggcgcc
     1021 aattcggaga gcaggactca gatcagccag cattcttgga acgtggacgc tggttctttc
     1081 gtctcggata atctgaagcg cttccaaata ataatatctg taaatcggct cctctgaatt
     1141 cccaagctcc ataatgtaag ggagagccct gtcgtcccgg atagaattca gatacctgta
     1201 cgttgcttcc ctgatttcag aacttttatg ggtgacgaaa ggaataactg tatagaggaa
     1261 tttcctgtct cttcttttct caaatgaatt cagaagtctg acaaatacat cctcttcctg
     1321 aaatttttcc tggaaatacc attcatagtc gtcaatggaa tccggaaggt gcgaatctaa
     1381 gtcaaaaccg taagattcgt acagatctaa tatagccacc tgaatttctt tggaggtgct
     1441 cctctttttc agaatttcag aaactcgggg aagaaattcg tccatcccct ttgcacggaa
     1501 agtacgaaga gcttctaaag tctccttgcc tcccgatgta agtttg
//
LOCUS       DAOQGJ010001164         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61193_length_1544_cov_2.774345, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001164 DAOQGJ010000000
VERSION     DAOQGJ010001164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61193_length_1544_cov_2.774345"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PK683_16055"
     CDS             complement(<1..825)
                     /locus_tag="PK683_16055"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNH10011.1"
                     /translation="MNLYDWVQIFFYLVVLLLILFPLGMYMQYVIEEREIPFFRFFSS
                     LERKIYILTGTDPKREMSAGEYMGSVLGFNLVCFIILYLILVLQGYLGLNPGNFRGLS
                     WDLAFNTAVSFTTNTNWQAYSGESTLSSFSQMAGLGVQNFTSAATGMAVFFALTRALS
                     RTNSSVIGNFWFDLIRSILYILLPLSIIISTFLILQGTVQSFSNPVEAVTLEGGRQMI
                     PLGPAASQIAIKQLGTNGGGFFNTNSAHPFENPTPFSNWLQLLSILLIPAALPFSFG"
BASE COUNT          532 a          303 c          324 g          385 t
ORIGIN      
        1 accgaaagaa aaaggaaggg ccgcgggaat gagcagtatc gaaagcaact gaagccaatt
       61 tgaaaatgga gtcggattct caaaagggtg cgcgctgttg gtgttaaaaa aacctcctcc
      121 gtttgtaccc agctgcttga tggcaatctg agaggcggca ggaccaagag gaatcatctg
      181 tctgcctcct tccagtgtca ctgcttctac aggattcgaa aaactctgaa cagttccttg
      241 caggataaga aatgttgaaa ttataatgga aagtggaaga agtatatata aaatcgaccg
      301 aataagatca aaccagaaat ttcctataac ggaagaatta gttctggaga gagctctggt
      361 caaagcaaaa aatacagcca tccccgtagc ggctgacgtg aaattctgaa cacccagacc
      421 tgccatctgc gaaaaactgc tcagagtcga ctctccggaa tatgcctgcc agtttgtgtt
      481 tgtagtaaag cttaccgcag tgttgaatgc cagatcccag gaaagtcccc tgaagtttcc
      541 cggattcagt ccaaggtatc cctgaagaac aagaattaaa tataatataa tgaaacatac
      601 aagattgaaa ccgagaactg aacccatata ttctccggca ctcatctccc gttttggatc
      661 agttcctgtc agtatataga tttttctttc caatgaactg aaaaatcgga aaaatggaat
      721 ctctctttct tctatgacat actgcatata cataccaaga gggaaaagta taagcaaaag
      781 aacaactaag tagaaaaata tctgcaccca atcataaaga ttcatagtac cctcggaact
      841 atgcacaaaa aaatctgtct gcgggtaaac gattatatga actgactggt aaaaaagaat
      901 gagagtataa aatttgtgta aagattttca gcctatgcaa aacagaatat ggaagccaca
      961 gtcagcctcc tgccgcctgc gttctgatca gagcattctg taacccttaa caaggctggt
     1021 cagatttatc cctatccccc ttgttttcct gaacaagata ctcatccgat gcggaaaagg
     1081 gaaaagccaa cgctagaaga atgcctcgcc ccttttcctt tcaggaaagg gcacaggtag
     1141 agaatttgtg ccacaagtag gcagggaata gggttaagag taactgctgg aaattctagg
     1201 cggtaagcct tgtattaatg ggaccagaat cagcgtagat aagattctgg aatggctagc
     1261 aacaggtgca gcgattcagg atatttgcaa aagttatacg tatttaaatg aaaaatcagt
     1321 aacacaggca atctgatacg cagcaaacag gcttaaaaat gaatcttttt ttgagttaaa
     1381 aaaatctgcc tgaagtatga ttttaaaaga tttccagttt cttacggatg aaaatataca
     1441 tcaggacgtt gtttcctatt tgactcaaat agacaagaag tgtaaaatga acttatgcca
     1501 tcgacggaat tcaagataaa ttagaaagaa gtgctgctta aaat
//
LOCUS       DAOQGJ010001165         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61389_length_1541_cov_3.267833, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001165 DAOQGJ010000000
VERSION     DAOQGJ010001165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61389_length_1541_cov_3.267833"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..>1541
                     /locus_tag="PK683_16060"
     CDS             259..>1541
                     /locus_tag="PK683_16060"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNH10012.1"
                     /translation="MNKEYENTVSELRILFHSGNIGSINEGLKRVTELNDVSLEEELL
                     SGTLYVNQPGGGGFSEWSISRNKFFTPPKSENIFEMNELEGNLWVGLISLLGRSQCST
                     AVRLKKEIKSLSFFGCNMPYIPSEIGSFSNLEILIVNNYSPSAFEEEKVNPLPPEIGN
                     LKKLKWLDMSQNRGLSPPPEIGKLKRLKTLLISANIITEITDEIWNLKNLRTLNLENN
                     RLTSVSEKIENLTELKTLKLLGNDLKTLPPQIGKLLKLEELEIMYNPLESLPEEMENL
                     KKLKPFLLVNTKLKEFPPVLRRLKKTAPMEEDFFPWLTGDIPLQIQNSFAEEVLNFEE
                     SNLKFLNLGSFGYPKAAAAWKNIILCSTDAHILIWNADNFELLWKTSAEGFHILSISE
                     DGKFFLCASENGLIRRYNISQSEAEFVWEEKSEDLL"
BASE COUNT          490 a          309 c          321 g          421 t
ORIGIN      
        1 aatgtttctg ctcacccgac agacggcaca ctcttcggta ctgccacatt cgacatcatc
       61 agtatatgaa acatcgaatc aggaaaaaaa actgaagaat tcagtctctt ttccgatttt
      121 tactcagtga gcagtctttc attttcagaa aacggttttc tctgtgcact gattttctat
      181 caaggttatg aggaacagaa agtgggaagc tcctacattg ctttgcttaa tctttctgaa
      241 tagaagagat ataatatcat gaataaagag tatgaaaata cggtctctga actgagaatt
      301 ctttttcact cagggaatat cggcagtatt aatgagggac tgaaaagagt caccgagctg
      361 aacgatgtgt ctctggaaga ggagcttctt tcagggactc tgtatgtcaa tcagcctgga
      421 ggaggaggtt tttcagaatg gtcgatcagc agaaataaat tcttcactcc cccaaagtct
      481 gaaaacatct ttgaaatgaa tgaactggaa ggaaatctct gggtcggact gatatctctt
      541 ctgggaagaa gtcagtgcag cacggcagta aggcttaaaa aagaaatcaa gtctctttct
      601 tttttcggat gcaatatgcc gtacattcct tccgaaatcg gcagtttttc gaatctggaa
      661 attctcattg taaacaatta cagtccctct gcattcgaag aagagaaagt aaaccctctt
      721 ccgcctgaaa tcggaaatct gaaaaaactg aaatggctgg atatgagcca gaaccgagga
      781 ctttccccgc ctccggaaat cggtaaactt aagagactga aaactctttt aatcagtgca
      841 aatattatta cggaaattac tgatgaaatc tggaacctga aaaatctccg cacactgaat
      901 ctggagaaca acagactgac ttcagtttca gaaaagatag aaaatctgac ggaactgaag
      961 acactgaagc tcctcggaaa tgatttgaaa acactgccgc cgcagatcgg aaaacttctt
     1021 aaactagagg aactggaaat catgtacaac cctcttgaga gtcttccgga agaaatggaa
     1081 aatctgaaaa aactgaaacc gtttcttttg gtcaatacaa aactgaaaga gtttcctccg
     1141 gttctccgca ggctgaaaaa aacagcaccc atggaggagg atttttttcc ctggcttacg
     1201 ggcgatatcc ctcttcagat ccagaattct tttgcagaag aagttctgaa ttttgaagag
     1261 tccaatctga aatttctgaa tctgggttct ttcggatatc ctaaggcagc ggctgcctgg
     1321 aaaaatatca tcctctgcag tacggatgca cacattctga tttggaatgc tgacaatttt
     1381 gaacttctct ggaaaacctc tgctgaagga tttcacattc tgtccatttc cgaagacgga
     1441 aaattttttc tctgtgcttc agagaacgga ctgattagaa gatataatat atcccaaagt
     1501 gaagcagaat ttgtctggga ggagaaatca gaagatcttc t
//
LOCUS       DAOQGJ010001166         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61432_length_1541_cov_1.946837, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001166 DAOQGJ010000000
VERSION     DAOQGJ010001166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61432_length_1541_cov_1.946837"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1541)
                     /locus_tag="PK683_16065"
     CDS             complement(<1..>1541)
                     /locus_tag="PK683_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10013.1"
                     /translation="TELFDLLLFCAGNTARNAESILDISFLLDHPEKEKLAELCISKI
                     SGTETCRICLAIPRNKLYYRDFEVPKEILRLVPEMDFTEETPGCRRVCSVCGRRYHYR
                     FITEYEDMSSSTEIRIEKLPPELSELPEEKEYLDQFLPVLHREMTHPEPKVRENAGFD
                     LSVYYYLTEDYQNLFSLLGNFQDLSVQRGAVQGISGRKLSEELVSVLSELLKSPDSGI
                     RSNAAEELASYYMQTDNIEGIKNLLSASDHIFTVSPTIQKAFFLRKESKIHLDILYPK
                     FLNLLNSSDSAVRRFADYFLAEMKPPEGSEEKIFLERLSHRNPEIRKEAALSLERKIS
                     KDFDPSFAFRPLLRLLKNRTTAWAAVSAFKKTAFLNYDLSRIIPFLIRQIKDRKSGGR
                     EDAVWTLSVIEEKRGFPDEFLKSLSPLLSFREDNISYMAYNIFESAEKRGKDISFAEK
                     HIRNFFQSRKENDYLKERSVYLLTDICIQKKDADRLKILLLHRSASVRYAASSRIYDS
                     LQRGK"
BASE COUNT          407 a          334 c          314 g          486 t
ORIGIN      
        1 tcctttcctc tctggagtga atcgtaaatt ctcgaggatg ctgcataacg gacggaagcg
       61 ctcctgtgca gcagaagaat tttcagcctg tccgcatcct tcttttgaat gcatatatcc
      121 gtcagaagat acactgatct ttctttcaga taatcgttct cttttctgga ctgaaaaaaa
      181 ttgcggatat gtttttcagc aaaggaaata tcctttcctc ttttttccgc agattcaaat
      241 atattgtatg ccatgtagga tatattgtcc tcacggaagg aaagcagcgg agaaagagat
      301 ttcagaaact catccggaaa ccctcttttt tcctcaatca ctgaaagagt ccagactgca
      361 tcttctcttc cgccgctttt cctgtctttg atctgtctga tcagaaacgg aatgattctg
      421 ctgagatcat aatttaggaa ggcagttttc ttaaaagcgg atactgccgc ccaggccgtt
      481 gttctgtttt ttaaaagccg aagcagagga cggaaggcaa aggagggatc aaaatctttt
      541 gagatttttc tttccagaga cagcgcagcc tcttttctga tttccggatt tctatgactg
      601 agtctctcca ggaagatttt ctcttcagat ccttcaggag gtttcatctc tgcaagaaaa
      661 taatcagcaa aacgcctgac tgcactgtca ctgctgttca ggagattcag gaattttgga
      721 tataaaatgt ctaaatgtat tttactttct ttacggagaa aaaatgcttt ctggatggtc
      781 ggactgaccg taaagatatg gtctgaggca gacagcagat tttttattcc ttcaatattg
      841 tctgtttgca tataataact ggcaagttct tccgctgcat ttgaccttat ccctgaatcc
      901 ggagatttca gaagttctga taggacggaa acaagttctt ctgaaagttt tctccctgag
      961 attccctgaa ctgcccctct ttgaacagac agatcctgga aatttccgag cagactgaaa
     1021 aggttttgat aatcttcagt cagataataa tatacagaga gatcaaatcc tgcattttct
     1081 ctgactttcg gttcaggatg agtcatttcc ctgtgcagga caggaagaaa ctgatccaga
     1141 tattcttttt cttcagggag ttctgaaagc tccggaggca gtttttcaat gcggatttct
     1201 gtgcttgagc tcatatcctc atattccgta atgaatctgt aatgatacct tcttccgcag
     1261 acagaacaga ccctcctgca tccgggcgtc tcttccgtaa aatccatttc cggaacaagt
     1321 ctgagtattt ccttcggaac ctcaaaatcc cggtaataca gtttattcct cggaatcgca
     1381 agacagattc tgcaggtctc tgttcctgaa attttactga tgcagagttc tgccagtttt
     1441 tctttttcag gatggtctag gaggaagctt atatccagaa tcgattccgc atttcttgct
     1501 gtgtttccgg cacagaagag cagtaaatca aaaagctctg t
//
LOCUS       DAOQGJ010001167         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61705_length_1537_cov_3.185560, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001167 DAOQGJ010000000
VERSION     DAOQGJ010001167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61705_length_1537_cov_3.185560"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..870)
                     /locus_tag="PK683_16070"
     CDS             complement(100..870)
                     /locus_tag="PK683_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10014.1"
                     /translation="MKKFIQKILNLFRKKREEIPAETEDLIGPLSFDEELKSLRKKFQ
                     DFLLTSRVDSGTVVKRRNYSLSKNGPTLFRLEGTESSGKNFSIAVVTGSQLQSTEEKT
                     GGLIVVTEAQLNKAIQKEYATLEGFLSNFKDLNLNDSSYQETKKNGWKEIASWKTFYL
                     EQIFLKLRPDEIAILLVVLDDTFRSTFMKCSSRRLKEIIRNELFFLNQSVNSADLNPN
                     SSNAELGDYDKVCSRFFSVKREVEKKMEKDAEENTQRR"
     gene            complement(944..>1537)
                     /locus_tag="PK683_16075"
     CDS             complement(944..>1537)
                     /locus_tag="PK683_16075"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNH10015.1"
                     /translation="MAEEARFKDLLNRFIREQNILTKFLSNFINIPEIIRSYGEKLKR
                     GESAESEEESYLDEVVTVISLSADKYILSWQNLSSEQKADAVEALVFKLKEAFLDITV
                     RFIMEHKDYLKSQRTLAEYEPVREVFYLIESRIDENVSHFIGEKISARLKSQDPRDFR
                     ERLEWQTRNVLESIRINGTILGFALGALIGTVHFFIH"
BASE COUNT          385 a          365 c          288 g          499 t
ORIGIN      
        1 ccggatgcgc ctgccagcac atggattctt cccggttcca ggctgaatga aattccgtcc
       61 agtatgacag tttcaccctt ttctgcacag agcctcagat tatctgcgct gagtgttttc
      121 ttccgcatct ttttccatct ttttttccac ttctcttttc acagaaaaaa agcgggagca
      181 tactttgtca taatctccaa gttcagcatt ggaactgttc ggattcaaat ctgctgaatt
      241 cacactctgg ttcaggaaaa acagttcatt tctgataatt tccttcaatc ttcgcgagga
      301 acatttcata aatgtactcc gaaaggtatc atcgagaaca acaagaagaa tggcaatttc
      361 atcaggacga agtttgagaa aaatctgttc cagataaaat gtcttccatg atgcaatttc
      421 tttccatccg ttcttttttg tttcctgata ggaggaatca ttcagattca aatccttgaa
      481 attgctcaga aacccttcca aggtagcata ctccttctga atcgccttgt tcagctgagc
      541 ttccgtcaca acaatcagtc cgccggtctt ttcttcagtg ctctgaagct gtgaacccgt
      601 gacaacggca attgagaaat tttttccgga agactctgtg ccttcgagcc ggaaaagagt
      661 cggaccgttt ttggaaagcg agtagtttct tcttttgact acagttccgg aatcaaccct
      721 ggatgtaaga agaaagtcct gaaatttttt tctgagactt ttcaattcct catcaaaact
      781 cagagggccg atcaggtctt ctgtttctgc agggatttcc tctctttttt ttctgaacag
      841 attcagtatt ttctgaatga attttttcat tgaactttca gctcctcgtt ctttttcaaa
      901 gcttgttccg actcatcttt tgaaaagatt ttttcagacc gcctcagtga atgaaaaaat
      961 ggacagtccc gatgagtgca cccaatgcaa atccaagtat ggttccgttt atcctgatgc
     1021 tttccagcac atttctggtc tgccattcca gtctttctct gaagtccctt ggatcctgac
     1081 ttttcagcct tgcggaaatc ttttcgccga tgaaatggga tacattctcg tcaatcctgc
     1141 tctctataag ataaaagact tctctgaccg gctcgtattc cgcaagggtt ctctggcttt
     1201 tcagatagtc tttatgttcc ataataaaac ggactgtgat gtccaaaaat gcttctttca
     1261 gcttgaatac cagggcttcc acagcgtctg ccttctgttc tgaagaaaga ttctgccagg
     1321 aaagaatgta cttgtctgca ctgagcgaaa tgacagtgac cacctcatcc agataggact
     1381 cctcctcgct ttctgcagac tcgcctcttt tcagtttttc cccataactt cggatgattt
     1441 cagggatatt gatgaaattg gaaagaaatt tagtcagaat attctgttcc cttatgaacc
     1501 ggttcagaag atccttgaac ctggcttcct ctgccat
//
LOCUS       DAOQGJ010001168         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61707_length_1537_cov_3.073549, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001168 DAOQGJ010000000
VERSION     DAOQGJ010001168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61707_length_1537_cov_3.073549"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..633
                     /locus_tag="PK683_16080"
     CDS             163..633
                     /locus_tag="PK683_16080"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNH10016.1"
                     /translation="MKKVLFSAAIFVFSFFGCSDPGPAEIVSGTEKCAHCSMGIINEK
                     YHSQIVTFKGRHIHFDSIECMYAYKIQNEDKVRKAWVHDFSRTKEWIETENAKFLKSE
                     KLLSPMAGNLSAYSSDSDLKSDLEKFGGRQYTGKEIADFVKAEWSKTGSALKQK"
     gene            676..>1537
                     /locus_tag="PK683_16085"
     CDS             676..>1537
                     /locus_tag="PK683_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNH10017.1"
                     /translation="MLHAETGIGDFSFLSLKALVPAFIYFSFFFPADSAVIRVCRDCG
                     LRSVQEGLKKAQAGDTLEVHSGTYAEGTVEIRKPLHLKGIGRPVIDGGRKGHVFDIQS
                     DSVTVEGFKIVKGGVSDIREFAGIHTENIRDCRFINNELEDNAYGFYLAKTEKCTLKG
                     NSSVGNAKDEVSGGNGIHLWYCTDISIIENEVRAHRDGLYLEFSNHLLIEKNFSTESI
                     RYGLHFMFSSENRFYRNRFSRNSTGTAIMYSKKIEVRENSFEKSKDLNSYGILLKDIS
                     DSDFSGNVFQE"
BASE COUNT          481 a          263 c          356 g          437 t
ORIGIN      
        1 tgattcttct tgcagtcttt ggacttctgg cttacattgt atggtcggag agaaaggcat
       61 ttctttctga aaaggaacag aactgatgaa aaaaaacgaa tattgtgctt ttgagactgc
      121 ttttctctgc cgattctgtg aaggaatttt ggagatttta tcatgaaaaa agtattattt
      181 tctgccgcaa tattcgtttt ttcctttttc ggatgcagtg atcctgggcc tgctgagatt
      241 gtgtccggca cagaaaaatg tgcacactgt tcgatgggaa ttataaacga aaaatatcat
      301 tctcagatag tcacattcaa aggacgccat attcattttg attccataga atgtatgtat
      361 gcatataaaa ttcagaatga ggataaggtc cgaaaagcct gggtccatga tttttcgaga
      421 acgaaagaat ggattgaaac tgaaaatgca aaatttctga aatcggaaaa gcttctttct
      481 ccgatggcag gaaatctgtc tgcctattct tccgattctg atctgaaatc ggatcttgaa
      541 aagttcggag ggcggcagta cacaggaaag gaaatcgcag atttcgtgaa agcagaatgg
      601 tcgaaaaccg gttccgcgtt gaagcagaaa taatttagta atattaaaat aagtatgaat
      661 ataatatata aaatattgct gcatgctgaa acaggaatcg gagatttttc atttctgtca
      721 ctgaaggctc ttgtacctgc ttttatttat ttttcctttt tttttcctgc tgattctgct
      781 gtcatcagag tatgcagaga ctgcggactg cgttcagtgc aggaaggact gaaaaaggcc
      841 caggcagggg atactctgga agttcattct ggcacttatg cagaggggac tgtggaaatc
      901 agaaaaccgc tgcatctgaa aggaataggc agaccggtta ttgacggagg cagaaaaggg
      961 catgtttttg acattcagtc tgattctgtc actgtggaag gattcaaaat tgtaaagggc
     1021 ggtgtcagcg acattcgtga gtttgccggt atacatacgg aaaatatcag ggactgcaga
     1081 tttataaaca atgaactcga agacaatgct tacggtttct atcttgcaaa aacggaaaaa
     1141 tgcaccctga aggggaacag ttctgtcgga aatgcaaagg atgaggtatc aggcggaaac
     1201 ggaattcatc tatggtactg cactgatatc agtatcatcg aaaatgaagt gagagctcac
     1261 agggacggac tctatcttga attttcaaat catcttctga ttgaaaaaaa cttcagcacg
     1321 gaaagcatca ggtacggtct gcattttatg ttttcgagtg aaaacaggtt ttacagaaac
     1381 aggttctccc gaaactctac aggaacggcc attatgtatt caaaaaagat agaagtgcgg
     1441 gagaattcct ttgaaaaaag caaggatctg aattcctacg gaatacttct gaaagacata
     1501 tccgacagtg acttttcagg aaacgtcttt caggaga
//
LOCUS       DAOQGJ010001169         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61726_length_1537_cov_2.647099, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001169 DAOQGJ010000000
VERSION     DAOQGJ010001169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61726_length_1537_cov_2.647099"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK683_16090"
     CDS             <1..357
                     /locus_tag="PK683_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNH10018.1"
                     /translation="VIVGIEDDNPRYPDAPKIKEQILINPKITPLAGNGKGYWEGCLS
                     VPGMRGFVERPYKIRVEYRDLKWKEHSEIIEGFPAIVYQHECDHLEGILYVDRLKSSK
                     LFGYTEEVDTKGDELD"
     gene            complement(354..737)
                     /locus_tag="PK683_16095"
     CDS             complement(354..737)
                     /locus_tag="PK683_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH10019.1"
                     /translation="MELKLNSIGKIKIIEIHGKFDIENTEEFETLFQKQLDSSPTMLA
                     IDMNKLDYIDSSGIGTLIKSLNAIKNKKGELVLVGLKPMILNVFKLAKLDMFFQIMNN
                     DDFKAKYTDDDDKDIDQLLRGKIPT"
     gene            893..>1537
                     /locus_tag="PK683_16100"
     CDS             893..>1537
                     /locus_tag="PK683_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH10020.1"
                     /translation="MVDFSLTDEQKALRQLARDFAKNEIAPHAARLDETGEYPMDILK
                     KAWEVGLMNLHIKPEYGGSGMNDFEEMLTAEELNYACSGVATAILANNLALAPVLLGA
                     DEYVMKKFIEPITQEFTLCAYAVTEPGAGSDVAGIRTTAVRVGDEYVINGSKQWITNA
                     GVSDWMFVLTKTDPSLGHKGMTGFIIDSKSPGVIIGKKEKNMGQRCSDTRSVTFE"
BASE COUNT          455 a          279 c          340 g          463 t
ORIGIN      
        1 gttattgtcg gcatagaaga cgacaatcca aggtatcctg atgcgccaaa aatcaaggaa
       61 cagattctga tcaatcctaa gattactcct cttgccggaa atggaaaggg ttactgggaa
      121 ggctgccttt ctgtacctgg tatgagaggt tttgtggaaa gaccatataa aatcagagtg
      181 gaatatcggg atttaaagtg gaaagaacat tctgaaatta ttgaagggtt ccccgccatt
      241 gtatatcagc acgaatgcga tcatttggaa gggattctct atgtggacag attgaaaagc
      301 tctaaactgt tcggatatac agaagaggta gatacaaaag gcgacgaact cgattaagtt
      361 ggaatttttc ccctgagcag ctgatctata tccttgtcat catcgtctgt atactttgcc
      421 ttaaaatcat cattattcat tatctgaaaa aacatatcca gtttggcaag tttaaataca
      481 ttcaggatca tcggtttaag tcctaccagg actaattcac cttttttgtt tttgatggca
      541 ttcagtgatt tgatcaaggt tcctatgccg gaagagtcaa tataatccag tttattcata
      601 tcaattgcaa gcattgtcgg tgaagaatcc agctgtttct gaaaaagtgt ctcaaattct
      661 tccgtatttt caatgtcaaa cttgccatgg atttcgataa ttttaatttt tcctatgctg
      721 ttaagtttta actccattca agccttctat ctatgttata atttcgttgt gaaatgcatt
      781 ctgcgaagat ctatttttct ggagttctgc cagtggggaa gcaaaaaaaa aaatatagga
      841 aaatttttgg tctatttgac agcgttttct atccctgaag tctgtcctta ttatggtaga
      901 tttttcactt acagatgaac agaaagcgtt gcgacagctt gccagagact ttgcgaaaaa
      961 cgagatagca cctcatgcag cccgattgga tgaaacaggg gaatatccta tggatattct
     1021 gaaaaaagcc tgggaagtag gattgatgaa cctgcacatt aaacccgaat acggcggatc
     1081 aggcatgaat gattttgaag aaatgctgac tgcggaagaa ctgaattatg cctgttctgg
     1141 tgtggcaact gcaatccttg ctaataatct tgctttagcc cctgttcttt tgggcgctga
     1201 cgagtatgta atgaagaaat ttattgagcc gataactcag gagttcactc tgtgtgcgta
     1261 tgcagtgact gaacctggtg caggttccga tgttgcgggc atcagaacga cagctgttcg
     1321 tgtaggagat gagtatgtca tcaacggaag caagcagtgg attacaaatg ccggtgtcag
     1381 tgactggatg tttgtgctta caaaaacaga cccctcactt ggtcacaaag gaatgaccgg
     1441 ttttatcata gattccaaat cccctggtgt aatcattggg aaaaaagaga aaaatatggg
     1501 acagagatgt tctgacactc gttctgtcac ttttgaa
//
LOCUS       DAOQGJ010001170         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61861_length_1535_cov_3.441892, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001170 DAOQGJ010000000
VERSION     DAOQGJ010001170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61861_length_1535_cov_3.441892"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PK683_16105"
     CDS             <1..778
                     /locus_tag="PK683_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNH10021.1"
                     /translation="FLNNIGDFNSEPPVFLGDALTSKPKESFDYVLTNPPFGKKSSIT
                     VTNEEGEKQSLTYNREEFWTTTANKQLNFLQHIVNMLKIGGQGAVVLPDNVLFEGGAG
                     ETIRKRLMQDADLHTILRLPTGIFYAQGVKANVLFFEYKGSSKQIHTKDIWFYDLRTN
                     MNFSLKTNSIGKDDFADFIKCYNPENRHRRKESERFKKYSYDEIIKRDKTSLDIFWLK
                     DDSLSDMDNLPDPDILAKEIIINLEDALDNFREVIDSESP"
     gene            complement(828..>1535)
                     /locus_tag="PK683_16110"
     CDS             complement(828..>1535)
                     /locus_tag="PK683_16110"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH10022.1"
                     /translation="SFRSLEEVIERVSNAGKSIDEIGQKALQISENGEAEFAKVISQM
                     RHISNASNDASKILTLINNIAKQTNLLALNAAIEAARAGEEGRGFEVVSEEVSKLADS
                     SGKNARQISELITEMKNAVSLGEGQIQNSSASMKKITEIINKISYNIGQVREITKEQS
                     EMVTDSSTRTDKIQNISAELKKLAETEDANSRQLKTNIDKAVQLSSAIQERIHILNNT
                     IDKLQNMSDQYMELLNR"
BASE COUNT          418 a          299 c          329 g          489 t
ORIGIN      
        1 atttctgaac aatataggag acttcaacag cgaacctcct gtttttctgg gtgatgcact
       61 gacctccaaa ccgaaagaga gttttgatta tgtgcttaca aatccgccgt tcgggaagaa
      121 aagcagcatt actgtcacaa acgaagaagg ggaaaagcaa agtctcacat acaacagaga
      181 agagttctgg acaacaacgg caaacaagca gctcaacttt ctgcagcata tagtaaatat
      241 gcttaagatc ggcggacaag gagcagtggt tcttcctgac aatgttctgt ttgaaggcgg
      301 agcaggtgaa accatccgaa aacgtctgat gcaggatgcc gatctgcata caatattgag
      361 acttccaacc ggaatttttt atgctcaggg agtaaaagcg aatgtgcttt tctttgaata
      421 taaaggatca tccaagcaga ttcacacaaa agatatatgg ttttatgatc tcagaacaaa
      481 tatgaatttt tctctcaaga caaattctat aggaaaagat gattttgctg attttataaa
      541 gtgttataac ccggaaaacc gtcatagaag aaaggaatca gaaagattca aaaagtattc
      601 ttatgatgaa attattaaaa gagacaagac aagtctggat attttctggc tgaaagatga
      661 tagtctttcc gatatggaca accttccgga tccggatatt ttagcaaaag aaatcattat
      721 caacctggaa gatgcactgg ataacttcag agaagtgatt gattcggagt ctccctaagt
      781 gttggagatt tcgaatatca gaaggatctg atttttttgc aaaaacccta cctgttcaga
      841 agttccatat actgatcgga catattctgg agtttgtcga ttgtgttgtt caggatatga
      901 attctttcct ggatggcaga ggagagctgg actgccttgt ctatgttcgt cttgagctgt
      961 ctggagtttg catcttcagt ttctgcgagt tttttcagtt cggcggagat gttctgtatc
     1021 ttgtctgttc ttgtgctgct gtctgtcacc atctcggact gttcttttgt gatctctctt
     1081 acttggccta tattataaga aattttattg ataatttctg taattttctt cattgaggcg
     1141 gaggaattct gtatctgtcc ttcgccgaga gagactgcat ttttcatttc agtgatgagt
     1201 tctgatatct gtcttgcatt ttttcctgaa gagtctgcga gtttcgatac ctcttcagag
     1261 actacctcga agcctcttcc ttcttctcca gctcttgctg cttcgatggc tgcattcagt
     1321 gccagaagat ttgtctgctt ggcgatattg ttgatgagcg tcagtatctt cgacgcatcg
     1381 ttcgacgcgt tggatatatg cctcatctga gaaatgactt ttgcaaattc cgcttctccg
     1441 ttttcagaga tctgcagagc cttctgtcct atctcgtcaa tgctctttcc tgcattggaa
     1501 actctttcta tgacttcttc caggcttcgg aaaga
//
LOCUS       DAOQGJ010001171         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_61943_length_1534_cov_2.596349, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001171 DAOQGJ010000000
VERSION     DAOQGJ010001171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61943_length_1534_cov_2.596349"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PK683_16115"
     CDS             <1..729
                     /locus_tag="PK683_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH10023.1"
                     /translation="SFVSDLDGKKKSLLADLQQTEKYVYESALSAKDNLAVIDSSVKV
                     IQELIDTSLKIGISSEVINEIADRVSLLSINAGIEAAKAGEFGRGFSVVAKEISSLGE
                     KTIQNSKEIRLLVKKTAKSADEAKEMISRILNSFLLISEKINQTNERIKSIFELFREH
                     GRIQSDVLENYRNTSDINREIRLSVSELTNAVQAIGNSISEMNKSIQEAVRTDSRISD
                     FASLLKLRSSSLKEEISFFRTGQE"
     gene            840..>1534
                     /locus_tag="PK683_16120"
     CDS             840..>1534
                     /locus_tag="PK683_16120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH10024.1"
                     /translation="MDKAFLYILLFINRMLFYFFSDLSIVSVLFLLSATGAVFLFSAA
                     RKKIFEFNLVIFTETALFSAFLVSFGDREMRSYSFLWFFLSLFFCREALSGKSKKLLL
                     LFPALFTLLFSLGVTGIFPAFLHGQVHPLMYAVSVPLVCYVFFRSPDPIFDPDSLLSV
                     ILDSIPVNIFIKDMEGRFIFLNSRTAETLGSERENIEGKTDFDFLLKESAEKLRAFDK
                     EAEDAGGTTITRV"
BASE COUNT          431 a          290 c          338 g          475 t
ORIGIN      
        1 tcattcgtat ctgatcttga cggaaagaaa aagtcgcttc ttgcagatct tcagcagaca
       61 gaaaaatatg tgtatgagag tgcgctttcc gcaaaagaca atcttgcagt gatcgattct
      121 tcagtaaagg tcatacagga actgattgat acttcgctga aaataggcat atcttcggaa
      181 gtcattaacg agattgctga cagggtttct ctgctttcca tcaacgccgg cattgaggcc
      241 gcaaaggccg gggaattcgg aagagggttc tctgttgttg ccaaggagat ttcgagttta
      301 ggagaaaaga caattcagaa ttcaaaagag atccggcttc tggtgaaaaa aactgccaag
      361 tcggctgatg aggccaagga aatgatcagc aggattctga attcattcct gctgatttcg
      421 gaaaagatca atcagacaaa tgaaaggatt aagagcattt ttgaactttt cagagagcat
      481 ggaaggattc agtccgatgt gctcgaaaat tacaggaata catctgatat aaacagggaa
      541 atcagacttt ctgtttccga actgacaaat gcagtccagg cgataggcaa ttccatatcg
      601 gaaatgaata agagcattca ggaagcagtc agaacagatt caagaatctc cgattttgca
      661 tccttgttga aattgaggag cagttcactg aaagaggaga tttctttttt caggacaggg
      721 caggagtgaa tactgcagac tcagcacaat actgaacttt tctcaaaaat gtaattcgcc
      781 atgacttttt taattgcatt aaatgatttt cgggttactg tgatttaaaa gcttcagtta
      841 tggataaagc attcctgtac attctgctct tcatcaacag aatgcttttt tatttttttt
      901 cagacctgtc cattgtttct gtactttttc ttctttcggc aaccggtgct gtttttcttt
      961 tttctgctgc aagaaaaaaa atcttcgaat tcaaccttgt tatttttacg gaaactgctc
     1021 tgttctctgc atttctcgtc tcattcggtg acagggaaat gcgatcctat tcgtttctct
     1081 ggtttttttt aagtctgttt ttctgcagag aggcattgtc cgggaaaagc aaaaaactac
     1141 tgctgctgtt tccggcactg ttcactcttc ttttttcact tggagttaca ggaatatttc
     1201 cggcctttct gcacggacag gtgcatcctc tgatgtacgc agtatcagtt cctcttgtat
     1261 gttatgtttt ttttcgcagt ccggatccaa tttttgatcc ggattctctt ctgagcgtga
     1321 ttctggattc cattcctgtg aatatattca tcaaggatat ggaaggaagg ttcatctttc
     1381 tgaattccag aacagctgaa actttaggca gtgaaaggga aaacatagaa ggaaaaaccg
     1441 actttgattt tcttctgaaa gagtctgcgg agaaacttcg ggcttttgac aaggaagcgg
     1501 aagatgcagg agggaccaca attacccgtg tgga
//
LOCUS       DAOQGJ010001172         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62057_length_1532_cov_2.789438, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001172 DAOQGJ010000000
VERSION     DAOQGJ010001172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62057_length_1532_cov_2.789438"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PK683_16125"
     CDS             complement(<1..348)
                     /locus_tag="PK683_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10025.1"
                     /translation="MNFNEKVLNFFKPPEFPENAEKNRKAYVLNTAVLSGLVLVFFLM
                     GANLLRSGTNLKGVYLQSSALILFLVTRYFLFRGAVVFSGFLFLFSGFSILTLSMMTF
                     GTVRAPAFGIYLFL"
     gene            589..1500
                     /locus_tag="PK683_16130"
     CDS             589..1500
                     /locus_tag="PK683_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH10026.1"
                     /translation="MSRETEWSKLKSMHKGFPAKEVLLAQGDPANQMYILMEGKANVI
                     RNGKLIGSVQKRGEYLGEISFLMNTEYTASVVTETPCTMIRIERNEAESFLRHSPEVA
                     ISMAAKLAGRLAELNRKISNLGNTFLNDALLNAVLEEKQDRNRRTASDLNLSSLSSLE
                     AEIPAGLEIIVQNQPLSHLYILLEGEVEIIKNSQLLAIESEPGYYMGDVSVLRKTLPN
                     ATVRARTDCRMIQIPAEKLDSFLHHSPEVAISIARKICERILTINNTMEKLIQYADRN
                     GLSETSENISRDTGREIRKIRKTVKLV"
BASE COUNT          534 a          311 c          323 g          364 t
ORIGIN      
        1 cagaaagagg tagattccaa atgcaggtgc cctgaccgtt ccgaaagtca tcatagaaag
       61 tgtaagaatc gaaaatccgg agaacaggaa aaggaacccg ctgaaaacaa cggcccctct
      121 gaagagaaaa taccgtgtga cgagaaataa aatcagtgca gaagactgaa gatagactcc
      181 tttcagattc gttcccgatc tgagcagatt tgctcccatc agaaaaaaca caaggactaa
      241 tcctgacagc acagccgtat tcagaacata cgcctttctg tttttttcag cattttccgg
      301 aaattccggc ggcttgaaaa aattcaaaac cttttcattg aaattcactt ttttcctcag
      361 caattgagca gaacaggggt taaagaataa atgtcatttc ctcagcctaa aaccaatttt
      421 cggaatatct ccaaaatgtc atctctgtta agatatgacg aaacactgag ctcataaatt
      481 tttttatttt tgaatcaaaa gacttggaaa aagtaaaagg cagaaagagg aaaaataaaa
      541 ttcttgcaaa tccttgtgct gaaggtttag ttttcttgag aggaagtttt gagcagagaa
      601 acagagtggt ccaaactaaa atccatgcac aaaggttttc ccgcaaagga agttctcctc
      661 gcgcaggggg atccggcaaa tcagatgtat atactgatgg aaggcaaggc aaatgtaatc
      721 cgaaacggaa agctgatagg ttctgttcag aaaagaggtg aatacctcgg tgaaatttca
      781 tttttgatga atactgaata cactgcttcg gtagtgactg aaactccctg cacaatgatc
      841 cgtattgaaa gaaatgaagc agagagtttt ctccgccaca gtccggaagt tgccatttcc
      901 atggccgcaa aacttgccgg acgtctggca gaactcaaca gaaaaatatc caatttggga
      961 aatacatttc tgaatgatgc cttattgaac gcagtccttg aagaaaagca ggacagaaac
     1021 agaagaaccg ccagtgatct gaatctctca tcactgtcca gcctggaggc cgagattccc
     1081 gcaggtcttg aaatcattgt acagaatcag ccactgagtc atctttacat tcttctggaa
     1141 ggggaagtgg aaattatcaa aaacagtcag ttacttgcaa tagaatccga acccggctat
     1201 tatatgggag atgtatccgt tctgagaaaa acactgccga atgcaaccgt cagagccaga
     1261 acagactgca gaatgattca gatccctgcg gaaaaactgg attcattcct gcatcattcc
     1321 cctgaggtgg caatctcaat cgccagaaaa atctgtgaaa gaattctcac aatcaacaat
     1381 actatggaaa aactgattca atatgccgac agaaacggac tgtctgaaac atcggaaaat
     1441 atcagcagag ataccggaag agaaatcaga aagatcagaa aaacagtcaa acttgtatag
     1501 gtgaaagtat gaagaaaatt ttcagcgtcg ga
//
LOCUS       DAOQGJ010001173         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62118_length_1531_cov_3.340108, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001173 DAOQGJ010000000
VERSION     DAOQGJ010001173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62118_length_1531_cov_3.340108"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(451..1290)
                     /locus_tag="PK683_16135"
     CDS             complement(451..1290)
                     /locus_tag="PK683_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10027.1"
                     /translation="MKRLFIYSLLPVWMSLADCSMRSDFINTVKQDFKDNNHMSADIL
                     TEKDISHLPEAVRKYLRHTKSVGQPKVRNFRAEFTGGMRGNPRDDYMKLESVQYNFYE
                     NPSRYFYMIAKKMALPAFGLHMYKQGSATFQVRLLNLFTVVDAKGEKMNQAETVTLFN
                     DMCFIAPPTLIDRRIKWEKINDRSVKGIYTNGKISISAVLHFNEKGELVNFLSNDRFH
                     TDGVKYESYPWETPVETYKEFNGYLLPSRAKLVYKRSDGDFTYGELEYKSVKYNLNQI
                     EDE"
BASE COUNT          462 a          322 c          277 g          470 t
ORIGIN      
        1 ccatggcaaa acctgctgca agagaattta caataaactc cgccctggaa taaatttgcg
       61 attcttggct catatgaact cctgatgaga atatattttt taattaatca atagagctgg
      121 tttcaaagta tcagaactga ggttttttga aaatcagaaa attgttgttt ttgaactgaa
      181 cagaatatcg aggtccgaat gatgagagcg ttattatagg atgcaattac aatccgcctg
      241 gaaactatgt cggagaaaaa ccttactgag tccgttcttc gcatctatag aaattagatg
      301 ataaatatac ttattgactg cacaaaaata tatcatctgc gcgttgtgct gaacagcgca
      361 ggtgtcaaag cattcattgc ccaatacggt gtgcttttac acagttccag cctctttttt
      421 gaatattctg aactgaagaa ccaatggaaa ctattcatcc tctatctgat tcaaattata
      481 tttcactgat ttgtattcca gttccccata tgtaaaatca ccgtcagacc tcttataaac
      541 gagcttggct ctgctcggca gaagatatcc gttaaattcc ttgtaagttt ccacaggagt
      601 ctcccaggga taactttcat attttacccc gtctgtatga aaacggtcat tactgaggaa
      661 gttgacaagc tcgccctttt cattaaagtg cagtactgca ctgatagaaa tttttccatt
      721 tgtgtaaatg ccttttactg atctgtcatt gatcttttcc catttgattc gtcggtcaat
      781 cagtgtgggc ggagcaataa aacacatatc attgaacagt gtaactgtct ctgcctgatt
      841 catcttttcc cctttcgcat cgaccacagt aaaaagattc agcagcctta cctgaaaagt
      901 ggcggagccc tgtttataca tatgcagacc gaatgcagga agtgccatct ttttagcgat
      961 catataaaaa taacgggaag gattctcata aaaattatat tggacagatt caagtttcat
     1021 ataatcgtcc ctaggattac cgcgcatccc tcctgtaaac tctgctctga aatttctgac
     1081 cttgggctga cctacggatt ttgtatgacg caggtatttc cttacagcct ccggcagatg
     1141 agatatatcc ttctctgtca atatatctgc ggacatatga ttgttatcct taaaatcctg
     1201 cttcacagta ttgataaaat cagaccgcat tgagcagtca gccagactca tccagacagg
     1261 taataaagaa taaatgaata aacgtttcat tgttcctcct gattcaacag gcggatttta
     1321 acacttcagg agtcaagcgt ttcaaaatta tctggaatga ttttaatcgg tcagacatat
     1381 gaaggataag agaaatccgg caacagccaa aattctgaat ttctgttttc aaatcccgac
     1441 atctaaattc agcaggactg cagaaaaact tccgaaacag aatcttcccg tgccgcagtt
     1501 tttttcgact tcgatccggt taaatacatt a
//
LOCUS       DAOQGJ010001174         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62135_length_1531_cov_2.892954, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001174 DAOQGJ010000000
VERSION     DAOQGJ010001174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62135_length_1531_cov_2.892954"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            414..650
                     /locus_tag="PK683_16140"
     CDS             414..650
                     /locus_tag="PK683_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10028.1"
                     /translation="MSRIPDGEKFRIPVHFGAENGYFVRNNLIYSYWIDLKEMQEGES
                     KTGQTVEQFLKDPEADPRAKQWIKELLLRFKDKK"
     gene            660..>1531
                     /locus_tag="PK683_16145"
     CDS             660..>1531
                     /locus_tag="PK683_16145"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH10029.1"
                     /translation="MRNIFIAFFTALLLCSCGASTPAHKKSKSGIAVLETVRLGGLDH
                     SVLIRGADASGPIILVLHGFAVPMMPFAHLGYADDGGLIEQKYIVVNYDQRGAGKTSR
                     NDTDKSTYNINQYVQDAEELAAYLRKRFNRDKIFLQGVSWGSIIGMHLIQRHPDWFYA
                     YLAEGQASNMQAVYADAAKFVRTEAEAEKNEKAIADLSSAGLPDVSRTDSENIKSIEI
                     IVKWLDMYSQKKFHLQDMTGLFFKSIREAPEYSFLDMLALLRSMNSFTENNTVPMLKT
                     DLNKEVPEVKVPVY"
BASE COUNT          475 a          308 c          336 g          412 t
ORIGIN      
        1 tgataacagc tgcagcccgg ctggatgttc ggagaattta taaattttaa aggattttat
       61 catttttact cttaccgcct atatgcccga attttttagc ttacaggaaa ctgaaactca
      121 ttctcgttta ttcggactgc tctttatatc aaaaagttga agcaatgcac tgtccaaatt
      181 caaatataag acattttata atcattttat atgaaaatct ttctctttct tcattcaaat
      241 tattcactca cctgcttttg cggctgcacc ggcggtttgc aagcccccct gcctatacag
      301 gactgcactg cagatcagaa aaagaacctt cctttcagcc aagttctcag aaggaaaagc
      361 aaattattca gaaacttact gcagccggac gataatgaat gcaaggaaaa attatgagtc
      421 gaattccaga cggcgaaaag ttcagaattc cagttcattt cggagcagag aacggatact
      481 ttgtaagaaa caatctgata tattcctatt ggattgattt aaaggagatg caggaagggg
      541 aaagcaagac aggtcagact gttgagcagt ttttgaaaga tcctgaagct gatccgagag
      601 caaaacaatg gatcaaagaa ctccttcttc gttttaaaga taaaaagtaa ggaaaaaaaa
      661 tgagaaatat attcattgca ttttttactg cactgcttct ctgcagctgc ggtgcatcaa
      721 caccggccca caaaaaatca aaatcaggaa tcgcagtatt ggagacagtc cgtctgggag
      781 gacttgatca ttccgttctg attcgcggag cagatgcttc cggaccgatc atccttgtcc
      841 tgcacggatt tgcagttcct atgatgccgt ttgcacacct gggatatgcg gacgacggcg
      901 gtctgattga acagaaatac attgtagtaa actacgatca gagaggggca ggaaagacat
      961 cccgaaatga tactgacaag tctacatata atataaatca gtatgttcag gatgctgagg
     1021 aactcgcagc atatctgagg aaacgtttca accgagacaa gatttttctg cagggagttt
     1081 cctggggaag catcatagga atgcatctga ttcagaggca tccggactgg ttttatgcct
     1141 atcttgcaga gggacaggct tccaatatgc aggcagttta tgcagatgct gctaaatttg
     1201 tcaggacaga agcagaggcg gaaaaaaacg aaaaggcaat tgcggatctc agctcagccg
     1261 gtctgcctga tgtgtccagg actgattcag agaatatcaa gtccattgag atcattgtca
     1321 aatggctgga tatgtactca cagaaaaaat ttcatctgca ggatatgaca gggctgtttt
     1381 tcaaatctat ccgtgaggct ccggaataca gctttctgga tatgcttgca cttctcagat
     1441 cgatgaacag ctttactgaa aataatacag tacctatgct caagactgac ctgaacaagg
     1501 aagtgcctga agtgaaagtt cctgtctact g
//
LOCUS       DAOQGJ010001175         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62186_length_1530_cov_3.763390, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001175 DAOQGJ010000000
VERSION     DAOQGJ010001175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62186_length_1530_cov_3.763390"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PK683_16150"
     CDS             <1..1305
                     /locus_tag="PK683_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH10030.1"
                     /translation="VLNDFNWRRNGIPGPGNNQTKKTDREYIRNTTSLNTVTKELLAG
                     RMELGSRVFYTGVRDHLFDPLSEFSSGTPDSKADIQQYGVHLLPTVYWKEASQTFRFL
                     LANEKETFRRDKRNAIDQLIDKGTRKFRNHTVLQMQDEISLLKGRLILAPSVQKEIYT
                     DRFNDNTIPVPLDVTNLPKHRKITDYNIYRFSILGSLYKTDSASVSWKTNISQEKRIP
                     SFLELFGERGSILGNASLKPERSRNADTGPVLEYKTKDWNIRNTNSFYYKKIEDMILF
                     VPNSQFSLRPENVDRADIRGMEWSIKLNLKEKWKFSSTYTYQKAVNISDVSYLNGKYL
                     PLRPLHEWYGLAAYKFSRAEIGFEPTFIGASFRDRSNEYVNYQPSRWIYSLYFNWSVY
                     KTEEKELSVNLDLRNILDTRAYDIIGYPLPGRMFYLTVSGVF"
BASE COUNT          501 a          316 c          317 g          396 t
ORIGIN      
        1 gttctgaatg atttcaactg gagaagaaac ggaattccgg gtccgggaaa caatcagacc
       61 aaaaagactg acagagaata cattagaaat acaacatcat taaatactgt cacaaaggaa
      121 cttctcgcgg gaagaatgga actgggaagc agggtatttt atacaggagt gagagaccat
      181 ctgtttgatc ccctttctga gttttcttcc ggaacccccg actcaaaagc ggatattcag
      241 cagtacggag tgcatcttct tccgacagtc tactggaagg aagcatctca gacattccgg
      301 tttcttcttg ccaatgaaaa agaaacattc cgcagagaca agagaaatgc aattgatcag
      361 ctgattgaca agggaacaag aaaattcaga aatcacacag tgcttcagat gcaggacgaa
      421 atctcccttc tgaagggaag actgattctt gcaccctctg tgcagaagga aatctataca
      481 gacaggttca acgacaatac aatccctgtt cctcttgatg tcaccaatct tccaaaacac
      541 agaaagataa cagactataa tatatacaga ttttcaattc ttggatcatt gtataaaacc
      601 gacagtgctt ccgtgagctg gaaaaccaat atatctcagg agaaaaggat tccctccttc
      661 ctggagcttt tcggtgagag agggtctatt ctgggaaatg cctccctgaa accggaaaga
      721 agcagaaacg ccgataccgg ccctgtcctg gaatataaaa cgaaggactg gaacatccgg
      781 aatactaatt cattctatta taaaaagata gaagatatga ttctttttgt tccgaactct
      841 cagttcagcc tgagaccgga aaacgtggac agagcggata ttcgcggaat ggagtggagc
      901 atcaagctga atctgaaaga gaaatggaaa ttcagttcaa catacacata ccagaaagca
      961 gtcaatatat ctgatgtatc ctatttaaat ggaaaatatc tccctctccg tcctcttcat
     1021 gaatggtacg gacttgcagc ctataaattc agcagggcgg aaatcggatt tgaacccaca
     1081 ttcatcggag catcattccg ggacagaagc aatgaatatg tcaactacca gccctccaga
     1141 tggatttaca gcctctactt caactggtca gtttacaaga cagaggaaaa agaactgagt
     1201 gtgaatctgg atctgagaaa catactggat actagagcct atgatattat aggataccct
     1261 ctccccggaa ggatgtttta tcttacagta tcgggagtat tctgatttta cttccattga
     1321 tattcagggt atgaaaagat ctcgataggc attcagacat tttttgattt tcttgacaca
     1381 atggtattca aggccattct tgctttatga aaagactccg ataggaattc agacgttttc
     1441 taaaatgatt gaagaaaact gcttctatgt tgataagacc aatttccttg cagatccggc
     1501 aaatacagaa cagatttgac tgttcttttc
//
LOCUS       DAOQGJ010001176         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62226_length_1530_cov_2.296271, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001176 DAOQGJ010000000
VERSION     DAOQGJ010001176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62226_length_1530_cov_2.296271"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PK683_16155"
     CDS             <1..627
                     /locus_tag="PK683_16155"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-4 subunit G"
                     /protein_id="HNH10031.1"
                     /translation="DCGKCIQCGICTDLCPDERLVNSGFTHIFAFRKEELKVRFQKGD
                     FIPEKPEIPENVLIFQEMTEGKGICYREIAAAGNNSVECELNASFNNLFDSEGQGVRS
                     VASPKHADVLLYSGPVSENMAGPLNTAWECMPEPKAMAAAGSEAVAGGLFQLGRLPKE
                     PDVFIGGDPPRPDVMIQAMRMLMGKFKYSFQSAFHKRIQELKNRRKQS"
     gene            753..1250
                     /locus_tag="PK683_16160"
     CDS             753..1250
                     /locus_tag="PK683_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10032.1"
                     /translation="MKYYKLQNPGIDQTPFLDSKCDWFGKMYSEETGGQILIVSSGRR
                     NAASQAKAMFENYKNEVTKNAAPISYRLKQAEQEIRESFMNGFKTLKKPDAEIIKDMT
                     AVINSQIQREILISNHLPTKNNADGTANDFSLQNEYAVMDAICKEYGIYIIKEPKCIH
                     ITWPA"
     gene            complement(1329..>1530)
                     /locus_tag="PK683_16165"
     CDS             complement(1329..>1530)
                     /locus_tag="PK683_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNH10033.1"
                     /translation="FVQSFDSERQMDFCENLSYTPWHSLPEHRPLGSINRARKDVYLD
                     IQKFRHEKNGAVNKEPDGKEKF"
BASE COUNT          465 a          307 c          357 g          401 t
ORIGIN      
        1 gactgcggga aatgcattca gtgcggaatc tgtacagatc tctgtcctga tgaaagactc
       61 gtcaattcag gattcactca tatatttgca ttcaggaagg aggagcttaa agtccgtttt
      121 cagaaaggag actttattcc tgaaaaaccg gaaattccgg agaatgtact tatatttcag
      181 gaaatgacag aagggaaagg gatctgctac agggaaattg cagctgcagg aaacaattca
      241 gtggaatgcg aactgaatgc aagtttcaac aatctttttg acagtgaggg gcagggtgta
      301 aggagcgttg catctcctaa acatgcagat gtgcttctgt attcaggtcc tgtcagtgag
      361 aatatggctg gacccctcaa tactgcatgg gagtgtatgc cggaaccgaa ggcaatggct
      421 gccgccggtt cggaggctgt tgcaggagga ctctttcagc tcggccgact tcccaaggag
      481 cccgacgtct ttattggagg cgatcctccc agaccggacg taatgatcca ggcgatgaga
      541 atgctgatgg gaaaattcaa gtacagtttt caaagtgcat tccataagag gattcaggag
      601 ctgaagaaca gaagaaaaca gtcttaaaac tctgtcgcag agtcggcgga attgcctgtc
      661 tgctctgcgc ttttataaaa aagaattgac tgaacttttc attcaggtaa aaagagattc
      721 cgaaaagaac ctgcatttgc agagggggga taatgaaata ctataagctc cagaatcctg
      781 gaattgatca gacacctttt ttggacagta aatgtgactg gtttggaaaa atgtattcgg
      841 aagaaacagg cggtcagatt ctgattgtga gctccggaag aagaaatgct gcaagtcagg
      901 caaaggcaat gtttgagaac tataagaatg aggtcactaa aaatgcggcc ccgatttcct
      961 acagactgaa acaggcagaa caggagatcc gggaatcttt tatgaatgga ttcaagactc
     1021 tcaaaaaacc tgatgcagaa attataaagg atatgacagc tgtcattaat tctcagattc
     1081 agagagaaat actgatttcc aatcatctgc ctacaaaaaa caatgcggac ggaactgcaa
     1141 acgatttcag ccttcagaac gaatatgcag tgatggatgc gatctgcaag gaatatggaa
     1201 tctacattat taaggagccg aaatgcatcc atattacctg gcctgcctga atttttcagg
     1261 aatatcagac agctgagaaa ttttcttccg acttcagcct ccctttaaac gactgaagtc
     1321 ttcttctctc aaaatttttc ctttccgtcc ggttccttat ttactgcccc gtttttctca
     1381 tgtctgaatt tttgaatatc cagatagaca tctttccttg cccggtttat gcttcccaaa
     1441 ggtctgtgtt ccggtaagct gtgccaagga gtatatgaaa gattttcaca gaaatccatc
     1501 tgcctttctg aatcaaagga ctgcacaaaa
//
LOCUS       DAOQGJ010001177         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62256_length_1529_cov_3.696744, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001177 DAOQGJ010000000
VERSION     DAOQGJ010001177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62256_length_1529_cov_3.696744"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..415
                     /locus_tag="PK683_16170"
     CDS             158..415
                     /locus_tag="PK683_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10034.1"
                     /translation="MEPWNISNCPKKTKDREDWVKRCRSWCGQISNRNGFSLKTHYDC
                     PKCGFGYIYRTGNYSLSSSADVENTAVCCNPDCRYEWNEYS"
     gene            complement(412..1233)
                     /locus_tag="PK683_16175"
     CDS             complement(412..1233)
                     /locus_tag="PK683_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNH10035.1"
                     /translation="MKTKFEFFEIVQEGNIAVLYLNKPEKRNAMDWSFWNELPSAVAE
                     INSEKSIRAFVVAARGKSFSTGLDVTGFTEQFAPFLKDNSSDDRRKFLDLILHMQSGI
                     NAVYNSDKPSVAAVQKHCIGGGLDLISACDIRYCSEDASFSLREAKVAIVADMGSINR
                     LPAIIGQGNTRELALRGHDIDSKEAYRMGLVTKILPDADSLLAEAKKTANEIAENPSI
                     VIEGVKQVMRFSEGKPLEAGLNYVCVWNSSFLASKDFSRAVEGFKTKKRPVYNQD"
     gene            complement(1217..1396)
                     /locus_tag="PK683_16180"
     CDS             complement(1217..1396)
                     /locus_tag="PK683_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10036.1"
                     /translation="MMQFIAALTVIADILGTEKLKPDRKNDYFRIFFEMVIEALIKGL
                     TWMIPFLLSSHEDKI"
BASE COUNT          457 a          349 c          292 g          431 t
ORIGIN      
        1 tatatgcaga atcagcttat aaaatcggag acaataagac tgcatttgaa cagtggagca
       61 gactgctcga aacccctttc gatccgatat ttatggaaat tcagatcatc acactgaaaa
      121 agaaaattca gaaagtaaag aaacaggatg aatagcaatg gaaccctgga atatctccaa
      181 ctgcccgaag aagacaaaag acagagaaga ctgggtaaaa agatgcagat cctggtgcgg
      241 acagatttcc aacagaaacg gattttcatt aaaaactcat tatgactgtc cgaaatgcgg
      301 attcggatac atatacagaa ctggaaatta cagcctcagt tcaagtgcag atgtggaaaa
      361 tactgcagtc tgctgcaacc ctgactgcag atatgaatgg aatgaatact cttagtcctg
      421 attgtagaca ggtcttttct ttgtcttgaa cccttccacc gctcttgaaa aatcctttga
      481 tgcaagaaaa ctggaattcc agacacatac atagttcaga ccggcttcaa gaggttttcc
      541 ttcggaaaat ctcatcacct gcttcactcc ttctatgacg atcgaagggt tttcagcaat
      601 ttcatttgca gtctttttgg cttctgcaag aagagaatct gcatccggga gaatcttagt
      661 gacaagaccc atcctgtagg cttccttgga atcaatatca tgacctctca gagcgagctc
      721 tcttgtattt ccctgaccga tgattgcagg aagtctgttg atgcttccca tatccgcaac
      781 aattgccacc tttgcttcac gaagagaaaa acttgcatcc tcactgcaat accgtatatc
      841 gcaggcagaa atgagatcca gaccgcctcc gatacagtgc ttctgaactg cagcaacaga
      901 gggtttgtca gaattgtata cagcattgat cccgctctgc atatgcagaa tcagatcgag
      961 gaattttctt ctgtcatcgg aactgttgtc tttcaggaaa ggagcaaact gttcggtaaa
     1021 tccagtcacg tcgagtccgg tagaaaatga ctttcccctt gcagcgacca caaatgcccg
     1081 gatgctcttt tcagaattga tctcagccac agccgaagga agttcattcc agaaactcca
     1141 gtccatcgca tttctttttt ccggcttgtt cagatacaga acagcaatat ttccttcctg
     1201 aacaatctca aagaattcaa attttgtctt catgagatga aagtaaaaaa ggaatcatcc
     1261 aagtcaaacc tttaattagg gcttcaatga ccatttcaaa gaatatgcgg aagtaatcgt
     1321 tcttccggtc tggttttagt ttttctgttc caagtatgtc tgcaatgaca gtcaatgcag
     1381 caataaattg catcacacct gccgttcctg ccgcccaaaa atgttcagat cctgatatcc
     1441 tctaaaccta tccctttttt tatcagcgcg ttgtttcttt attggctctc ttcagtttta
     1501 ctttatattc tctcagataa tagtatcca
//
LOCUS       DAOQGJ010001178         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62279_length_1529_cov_2.837178, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001178 DAOQGJ010000000
VERSION     DAOQGJ010001178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62279_length_1529_cov_2.837178"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PK683_16185"
     CDS             <1..428
                     /locus_tag="PK683_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10037.1"
                     /translation="IQEEAEFMPLYESENTARSVSSASGFARTSEMQKLKWGLLKWRY
                     DRSSTVYRHFTSPAGKVSVSIPKNESDLLFESASALPVLFENQIYKIEIDPDYFLNDF
                     VSSEPKTLKDSQNSKEQENFKIPISVQKKNLADNGRNKN"
     gene            409..>1529
                     /gene="gltX"
                     /locus_tag="PK683_16190"
     CDS             409..>1529
                     /gene="gltX"
                     /locus_tag="PK683_16190"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNH10038.1"
                     /translation="MEEIRTRFAPSPTGFLHVGGARTALFNYLYARAKKGKFILRIED
                     TDQDRSTESSFQTIIESMKWLGMKWDEGPEIGGEFGPYKQSERLEIYKKYTDLLIEKN
                     LAYRCFCTPEELEAKKKNAEAMGIPNIYDGTHANLSEKEIREKLDAKIPYAVRFRTPA
                     KNLVVDDLIQGKVKFDTKLIGDFIIVKSDGFPSYNYAVVIDDCLMKISHVIRGVGHLS
                     NTPRQILIYEALGFKVPRFGHVSEIVGTDGKKLSKRAGATSILAFRDLGYSSECIVNY
                     MALLGWTSPDGQEYLPNKKSEEIFDVERCSKSPSMFDVFKKMKDEEKESVNLTSLTSS
                     ELADWLNPKSKLNWLSNRYIRDAEIRTVTKDILPFIKDIP"
BASE COUNT          507 a          305 c          326 g          391 t
ORIGIN      
        1 atatacagga agaagctgaa tttatgcctc tctatgaatc ggaaaacacc gcaaggtcgg
       61 tgtcctctgc atccggattc gcaagaacat ctgaaatgca gaaactgaaa tggggccttc
      121 tgaaatggag atacgacaga tccagcactg tgtaccgtca tttcacttct cccgcaggaa
      181 aagtatccgt ttccattcca aaaaatgaat cagatctgct attcgaatct gcatccgctc
      241 tgccggtgct ttttgaaaac cagatctata aaattgaaat agatcccgat tattttctca
      301 atgattttgt atcttctgaa ccgaaaacac tgaaggattc gcagaactca aaagagcagg
      361 aaaattttaa gattccaatt tcagttcaaa aaaaaaattt ggcagataat ggaagaaata
      421 agaactagat ttgctccttc tcctaccgga ttccttcatg tgggcggagc gagaacagca
      481 cttttcaatt atctgtatgc ccgcgcaaaa aaggggaaat tcattctgag aatagaggac
      541 accgatcagg acagatccac tgaaagttcc tttcagacaa tcattgagtc tatgaaatgg
      601 ctcggaatga aatgggacga gggtcctgaa atcggaggag aattcggtcc gtacaaacag
      661 tcggaaagac tggaaattta taaaaaatat accgatctcc tcatcgaaaa aaatctggcc
      721 tacagatgct tctgcactcc tgaagaactg gaagccaaaa agaaaaatgc ggaggcaatg
      781 ggaattccga atatttatga cggaactcat gcaaatcttt ctgaaaagga aatcagagaa
      841 aaacttgatg ccaaaattcc ctatgcggtg agattcagaa cgcctgcaaa gaatctggtc
      901 gtagacgatc tgattcaggg aaaagtgaaa tttgacacta aactgatagg tgattttatt
      961 atagtaaaat cagacggttt tccttcttat aattatgccg tagtgattga tgactgtctc
     1021 atgaaaattt ctcatgtgat caggggagtc ggtcatcttt ccaatactcc cagacagatt
     1081 ctgatctatg aggcgcttgg attcaaggtt ccaaggttcg gacatgtgtc tgagatagtg
     1141 ggaacggacg gaaaaaaact ttccaaaaga gcaggagcaa cttccattct tgcattcagg
     1201 gatctgggat acagttccga gtgcatagta aactatatgg ctcttctcgg atggacaagt
     1261 cctgacggac aggaatatct tccaaataaa aaatccgaag aaatattcga cgtggaaaga
     1321 tgttccaaat ctccttccat gtttgatgtg tttaaaaaga tgaaggacga ggaaaaggaa
     1381 tccgtaaatc tgacttcact gacttcttca gaactcgccg actggctgaa tccgaagtcc
     1441 aagctcaact ggctttccaa caggtatatc agagatgcag aaataagaac agtcacaaag
     1501 gacattcttc ctttcataaa ggatatccc
//
LOCUS       DAOQGJ010001179         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62290_length_1529_cov_2.657395, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001179 DAOQGJ010000000
VERSION     DAOQGJ010001179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62290_length_1529_cov_2.657395"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..956
                     /locus_tag="PK683_16195"
     CDS             48..956
                     /locus_tag="PK683_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNH10039.1"
                     /translation="MDQASGLRKLKDEIALPTKASISVVKKSSAKIYAVSSGKGGVGK
                     TTVTVNLAIALAKANKKVLVLDGDLGLANINVILGIIPKFTLYHVIKGHKTLQEVVLE
                     TAEGIDIIPGASGYSQLADLDSTARESLIQNIMEINDYDYILIDTGAGIGSIVISLIL
                     AADEVLIVTTPEPTSITDSYGLIKSITNRNKDFKLNVVINKAKDELEGKKVARRVIDI
                     SQKFLNVLPYEIGLIYRDEEVERSILAQKPFLVTAPRSRASDCMMRLSQQILNTTIET
                     PAVNDENRLTSYFRRFFHLADHKDGA"
     gene            959..1474
                     /locus_tag="PK683_16200"
     CDS             959..1474
                     /locus_tag="PK683_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004505214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10040.1"
                     /translation="MHIRVAGAFAIIGFIISLLSGITVGNRITHIFTVTLISTVLFGA
                     LGYGLYAFLQKQVPQFIDFLQELELNSGIYDYESGSEKETIESFSNTVSTGSKSGSSY
                     SSSIGGSDGYDTPRAPAKNGKFGDHIIVDNIEIKNEPKLMAEAIRTMIARDEMEGISS
                     SAAQQQTVPKK"
BASE COUNT          465 a          295 c          355 g          414 t
ORIGIN      
        1 aaacattcag tgtttctccc tgattgtatc ctgcaggtga tattgtaatg gatcaggcat
       61 ccggacttag aaaacttaaa gatgaaattg cactgcccac taaggcatct ataagtgtag
      121 ttaaaaagtc cagtgccaaa atatatgctg tcagttcagg aaaaggcgga gtcggaaaaa
      181 caaccgttac tgtgaatctg gcaattgcac tcgcaaaggc aaacaaaaag gtccttgtac
      241 tggacggaga tcttgggctt gccaatatca atgtcattct cggaataatc cctaaattca
      301 ctttgtacca tgtgatcaaa ggccacaaga ctcttcagga agttgtgctg gaaaccgccg
      361 agggcatcga cattattccg ggagcaagcg gttattcgca gcttgccgat ctggacagta
      421 ctgcacggga aagcctgatt cagaatataa tggaaatcaa tgactacgat tatatcctga
      481 ttgatacagg agcgggaatc ggttcaattg tcatcagtct gattctggcc gctgacgagg
      541 tgctcatagt gactactcct gaacctacat cgatcaccga ttcgtatgga ctcataaaat
      601 cgattacaaa caggaataag gactttaaac tgaatgtagt catcaacaag gccaaggatg
      661 aattggaagg gaaaaaggta gcaagaaggg tgattgatat cagccagaaa ttcctgaacg
      721 tacttcctta tgaaattgga ctgatataca gggatgagga agtggaaaga agcattcttg
      781 cacagaaacc ttttcttgtc actgctccca gatccagggc ctctgactgt atgatgaggc
      841 tttctcagca gattctgaat actacaattg aaacacctgc agtaaatgat gaaaacagac
      901 tgacctcata tttcagaaga ttttttcatt tggccgatca taaggatggt gcctgaatat
      961 gcatattaga gttgccggag catttgcgat tattggattt attatcagtc ttctgagcgg
     1021 gatcactgtg ggaaacagga tcactcatat ttttactgtg acattgattt ccactgttct
     1081 gttcggtgca ctgggttatg gtctttatgc gtttctgcag aaacaggtac ctcagttcat
     1141 tgattttctg caggaattgg agctgaattc cggaatatat gattatgaat ctggatctga
     1201 aaaggaaaca atcgaaagct tttcgaatac agtgtctacc ggctcaaaat cagggtcatc
     1261 ttattcctct tcgatagggg gatctgacgg atatgatacg cccagggctc ctgccaaaaa
     1321 tgggaaattc ggagatcata tcattgtgga caacattgaa atcaaaaatg agcctaagct
     1381 gatggctgag gcaatcagaa caatgattgc cagggatgaa atggaaggaa tctccagttc
     1441 agcagctcag cagcagactg tgccgaagaa gtaatgtgtc cttctttttt tgtggaaata
     1501 aaactctgat ttgccgataa tggaacagt
//
LOCUS       DAOQGJ010001180         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62363_length_1528_cov_2.418873, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001180 DAOQGJ010000000
VERSION     DAOQGJ010001180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62363_length_1528_cov_2.418873"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PK683_16205"
     CDS             complement(<1..872)
                     /locus_tag="PK683_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH10041.1"
                     /translation="MKLLSAIVEWSLANRLIVLAGTFIFVLYGINAARHLKIDAVPDV
                     TTIQVQVITSAPALSPLEIEQYVTFAVERTMAGIPRVEEVRSVSRYGLSVVTIVFKDG
                     TDIFHARQMVGERLKEAGEAIPRGYGTPFLGPISTGLGEIFQFTLRSEKHSLMELTTL
                     LNWYVNPMLKTVPGVVEVNTFGGESKQYQIILNTERMQSLGLSIRDVTEALQKNNAAM
                     GGGYIEHNKEHFLIGAEGLAKSLEDLKKIPLGRTKQGIPMTLGTVSDVRQGPKLRLGA
                     SSRDGIGEEVGGITL"
     gene            complement(872..>1528)
                     /locus_tag="PK683_16210"
     CDS             complement(872..>1528)
                     /locus_tag="PK683_16210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH10042.1"
                     /translation="MFLKNIMENVNTPVCRIFSRSGDAAVGRIYITSPLTGIALARDA
                     VPGAQVDASAVLGTVGDISEVWFMIKIFEKDLSKIREGTGARIILNAYPGETFEGRVD
                     FIGNQIDPGSRTVNGRIVLKNRESRAKIGLFGKALIDIQDDSVLQIRSSALFESDRKK
                     FVFVERSKGEYEAREVRTGRVSEDSLEILSGVIEGEKIIIKGVFSLKSVHLKSTFGEE
                     "
BASE COUNT          369 a          430 c          299 g          430 t
ORIGIN      
        1 agagtgattc cgccaacctc ctcgcctatt ccgtcacggc tagaagcacc cagtctgagc
       61 ttcggtccct gtctgacgtc gctcactgta cccagtgtca tcgggatgcc ctgttttgtc
      121 ctcccgagcg gaattttttt cagatcttcc agtgactttg cgaggccctc cgcaccgatc
      181 aggaaatgct ccttattatg ctcaatgtac ccgccgccca ttgccgcatt gttcttctga
      241 agagcttcag tgacgtcacg gatggaaaga cccaatgact gcatcctttc cgtattcagg
      301 atgatctgat actgctttga ctctcctccg aaggtattga cttccaccac tccgggcact
      361 gtcttcagca taggatttac ataccagttc agaagcgtag tcagctccat caggctgtgc
      421 ttttcgcttc tgagagtgaa ctgaaatatc tcgccgagac ctgtggaaat aggacccaga
      481 aagggagtcc cgtatcccct gggaatcgcc tctcctgcct ccttcagcct ttccccgacc
      541 atctgccttg catgaaaaat atctgttcca tccttgaaca caatagtgac tacgctgaga
      601 ccgtatctgg atacagatct cacctcttcc actctgggaa tgccggccat agtcctttcc
      661 actgcaaatg tcacatactg ctcaatctca agaggagaaa gtgccggtgc cgaagtgatg
      721 acctgaacct gaattgtggt cacatcagga acagcatcga tcttcagatg ccttgcggca
      781 ttgattccgt acagaacaaa tataaaagtc cccgcaagaa caatgagtct gtttgcgagc
      841 gaccattcta caatcgccga taaaagcttc attattcctc tccgaatgtg gacttcagat
      901 gcactgattt aagtgaaaaa acacctttta taatgatttt ttctccctca atcactcccg
      961 aaaggatttc cagggaatct tcagacaccc tccctgttct gacttctctc gcttcgtatt
     1021 caccttttga tctttctaca aagacgaatt tctttctgtc cgattcaaac agtgcggagc
     1081 ttctgatctg gagaacagaa tcatcctgaa tgtcaataag cgcctttccg aaaagaccta
     1141 tctttgcgcg gctttccctg tttttcagaa caattcttcc gtttacagtt cttgaaccgg
     1201 gatcaatctg gtttccgata aagtccactc ttccttcaaa agtctcaccg ggataagcat
     1261 tcagaatgat tctcgcacct gtaccttctc ttatttttga aagatccttt tcgaaaattt
     1321 ttatcatgaa ccagacctcg gaaatatctc cgactgttcc gagaaccgca gatgcatcca
     1381 cctgtgcgcc cggaaccgca tccctcgcca gagcaattcc tgtcagaggc gacgttatgt
     1441 agattctgcc gacagccgca tcgccgctcc ggctgaatat tcggcacact ggagtgttta
     1501 cattctccat tatattcttt aaaaacat
//
LOCUS       DAOQGJ010001181         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62365_length_1528_cov_2.363204, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001181 DAOQGJ010000000
VERSION     DAOQGJ010001181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62365_length_1528_cov_2.363204"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..>1528
                     /locus_tag="PK683_16215"
     CDS             86..>1528
                     /locus_tag="PK683_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH10043.1"
                     /translation="MSGKFSEETKLRSLNSMNLIRFGLFCFYVISLAAVHSIFLPAQI
                     ISHIIGVILYGLSAVYLHFRIKQNNVPAFLPVFVLFLDITIISVLNVYDASFGRVYAS
                     GIIKNPALYALYFFAIFYSAFLMNKQIVLTCGFYAALGNVFLNISAYASGLEYSEDPK
                     LFNETGYSSLSVEAVKSLFLFSFAYTIAVLMQILQDTIKKTNESLKQTDDMNRKLGVQ
                     STEILNTSAELSRTIEEWSGRISVFFNDIQHQNEQTAKINQFIDEFTLSQEKIKPLAG
                     LQSERSDRLSALTDTAQSKRETAIEKNRQLLQNMEIIQSSSHEIQDSLKEDQLSAEKL
                     HSFFNKLTEINGIITEISEKTNLLALNASIEAARAGTSGKGFAVVANEVGKLADFSSS
                     NAREISGIVKESRKVMKASELSKAEVNMNVNSQTAHLNTAMEMIRSISVLNEYMKQMN
                     DSYVESVSEFRSGTVSLSALVSANFEKSFLI"
BASE COUNT          480 a          303 c          318 g          427 t
ORIGIN      
        1 agagttgacc tgtgtgcaat ttatgtaaaa ttgctgcaga ctgccgaaaa aagttacagt
       61 cggcaggcag actccgggag aatgtatgtc aggaaaattc agcgaagaaa caaaactcag
      121 atcactgaac tcgatgaatc tgatccgttt tggactgttc tgtttttatg taatcagcct
      181 tgccgccgtg cacagcatct tccttcctgc tcagattatt tcccatatca tcggggttat
      241 tctatacggt ctgagtgcag tctatcttca cttcaggata aaacagaaca atgtacctgc
      301 ttttcttcct gtattcgtgc tttttctcga cattactatt ataagtgttc ttaatgtata
      361 tgatgccagt ttcggaagag tgtatgcttc aggaattata aaaaatcccg ctctttatgc
      421 tctgtatttt tttgccattt tttattcagc attccttatg aataagcaga ttgtgctgac
      481 ctgcggattt tatgcagctc tcggcaatgt attcctgaac atttctgctt acgcatcagg
      541 tctggaatat tcagaagatc cgaaactgtt caatgagacc ggttacagtt cactcagcgt
      601 ggaagcggtc aaatcacttt ttcttttcag ttttgcatat acgattgctg tgcttatgca
      661 gatactgcag gatacaataa agaagacaaa tgaatcactc aagcagacag atgatatgaa
      721 cagaaaactt ggagtgcaga gcactgagat tctgaacaca tcggcagagc tcagcagaac
      781 cattgaagaa tggagcggca gaatatcagt attttttaat gacattcagc atcagaatga
      841 gcagactgca aaaatcaatc agttcattga tgaattcact ctttcacagg aaaaaatcaa
      901 accccttgca ggacttcagt ccgaaagatc ggacagactt tcagccctca cggatactgc
      961 acagtctaaa agagaaaccg ccattgaaaa gaacagacag ctccttcaga atatggaaat
     1021 cattcagagc agcagtcatg aaatccagga ttcactgaaa gaagaccagc tgtccgcaga
     1081 aaaacttcat tcattcttca ataaactgac tgaaatcaac gggattatca ccgagatttc
     1141 agaaaagacg aatctgcttg ctctgaatgc atctattgaa gcagcacggg caggcacctc
     1201 aggaaaaggt tttgctgtag tagcaaatga ggtaggcaaa ctggcagatt tcagcagttc
     1261 aaatgcaagg gaaatttcag gcattgtcaa ggaaagcagg aaggttatga aagcatcgga
     1321 gctttcaaag gctgaagtca acatgaatgt gaattcacag actgctcatc tgaatactgc
     1381 aatggaaatg atcagaagta tttcggtcct gaatgagtat atgaaacaaa tgaacgattc
     1441 ttacgtggaa tccgtatctg aattcagatc aggcacagtt tcgctttctg ctttggtaag
     1501 tgcaaatttt gaaaaaagct ttttaata
//
LOCUS       DAOQGJ010001182         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62426_length_1527_cov_2.724864, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001182 DAOQGJ010000000
VERSION     DAOQGJ010001182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62426_length_1527_cov_2.724864"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1527)
                     /locus_tag="PK683_16220"
     CDS             complement(<1..>1527)
                     /locus_tag="PK683_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10044.1"
                     /translation="IDTILKEQALVCQTDSLHGDLNASNILYGIGSVGGQISTAVIDL
                     YDINTKGNIFFDAARYETDIFLRTVQRYLKKNHKDILDKKDFSFEEQIFLLNFIDRLE
                     DSAYIKKSASSSQMNDSFFLYPEGDKPVFCLNILNIILQIFPKLNLQKDSSDKWTVED
                     IRKSFYLCMAVYSLKFSMFQKEDEAVKTAALFLSMRYFRRLEDMRSSAAQSGSLPDLT
                     IPETSSLSDLCKKSNDKFRNDVISPELFTDFYKEGISDKIWRFYKNDDKIFFISGEIG
                     SGKSVFLDFLTEEGQYTVPTLKISGKIELKDKFSLIDEIREKLRIDKTESLPVKIGKM
                     MKSSDKFFLVIIDELYYNSNQNLASQCLRQLFWETLSDGRFKFIISVIPAYWSRLTAN
                     EHLKDIIAEKAMSFEVQQIDEDSKYASDSSVNFFKFTAPENYWNDIVKKYFKYYSIKG
                     KLKPSALRILSTEIWILKLFCEVKRSSNVGELEHIYFVDVLDQCLNSYHQKIAASGGI
                     T"
BASE COUNT          449 a          287 c          277 g          514 t
ORIGIN      
        1 gtgattccgc ctgaagcggc aatcttctga tgatatgagt ttagacattg atcaagaaca
       61 tcgacaaagt atatatgttc aagttcaccg acgtttgaac tgcgctttac ctcacaaaat
      121 aattttaata tccagatttc agtggaaagt attcgaagag cagaaggctt cagttttccc
      181 tttatgctgt aatacttgaa atattttttt acaatgtcat tccagtaatt ttcaggagca
      241 gtgaatttga aaaaattgac tgaactgtcc gaagcatact tgctgtcttc gtcaatttgc
      301 tgaacttcga agctcatcgc cttttctgca ataatgtctt tcaggtgttc gtttgcagtc
      361 agtctactcc agtatgcggg tatgactgaa atgataaact tgaatctgcc atctgaaaga
      421 gtttcccaga aaagctgtct aaggcactga gatgcaagat tctgatttga attatagtat
      481 aattcatcta taattacaag aaagaacttg tcagaagact tcatcatttt accgattttt
      541 acaggcagac tttcagtttt gtctattcta agcttttcac ggatttcatc gattagagaa
      601 aatttgtcct tcagttcaat ttttcctgaa atcttcagtg tggggactgt atactgtccc
      661 tcctcagtca gaaaatccag gaaaacggat tttcctgagc ctatttctcc actaataaaa
      721 aaaattttat catcgttttt atagaacctc cagattttat cactgattcc ttccttgtag
      781 aaatctgtga aaagttcagg cgaaatgaca tcgtttctga atttgtcgtt tgattttttg
      841 caaaggtcac tgagtgaaga tgtttcaggg attgtcagat caggcaggga accgctttga
      901 gctgctgagc tgcgcatatc ttcaagtctt ctgaagtatc tcattgaaag aaacagagct
      961 gcggtcttta ctgcttcgtc ttctttctga aacattgaaa acttaagaga atacactgcc
     1021 atacacagat aaaaactctt tcttatatcc tcaactgtcc atttatctga tgaatctttc
     1081 tgtagattca gtttaggaaa aatctgaaga atgatattga gtatattgag acagaataca
     1141 ggtttgtctc cttccgggta caaaaaaaat gagtcattca tctgactaga acttgcagat
     1201 tttttgatat aggcagaatc ttccagcctg tctataaaat taagaaggaa tatctgttct
     1261 tcaaaactga agtctttttt atccaggatg tctttgtgat tttttttcag atatctctga
     1321 acagtcctca ggaaaatatc tgtttcataa cgggcagcat caaaaaaaat gtttcccttt
     1381 gtatttatgt catacagatc aattactgca gtgctgatct ggccgcctac actgcctatg
     1441 ccgtaaagaa tgttcgatgc attcaaatca ccgtgtagag aatctgtctg gcagacaagt
     1501 gcctgttcct ttaatatggt atcaatc
//
LOCUS       DAOQGJ010001183         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62472_length_1526_cov_3.297757, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001183 DAOQGJ010000000
VERSION     DAOQGJ010001183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62472_length_1526_cov_3.297757"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..448)
                     /locus_tag="PK683_16225"
     CDS             complement(26..448)
                     /locus_tag="PK683_16225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HNH10045.1"
                     /translation="MRIKSLYLICLLTLSSVSNCEKFGLLGQGGVPGSTAKTEIEAAA
                     NTSATLVYTTLCSSSSLSSSKSQCLSLASIGVIIEKILVPITAKISSSKHYTRSSVDD
                     CKSKVMTIGFLISGPGLSGITCNLKETPKVIQIGESGL"
     gene            1082..>1526
                     /locus_tag="PK683_16230"
     CDS             1082..>1526
                     /locus_tag="PK683_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH10046.1"
                     /translation="MKSAYRKNLVYGIIDDDRQFSSTLAGLLKLKNTTREVHQFMSAE
                     DALQSGILKDLDFLFVDSRLDGMDGITFLGQRQVRELSIPKLIISGFNAEGRIFDAMK
                     YGIAGYMFKEEMHLLNHILETIFSGGAFLSPSIAFRVLEYFRRDRT"
BASE COUNT          451 a          242 c          305 g          528 t
ORIGIN      
        1 attctttaga aatgatcagc catttttaaa gtccagactc cccgatttga ataaccttgg
       61 gagtttcttt cagattgcag gttatcccag acagaccagg gccagaaatc aagaatccaa
      121 ttgtcataac ctttgattta cagtcatcta cggaacttct cgtataatgc tttgaggatg
      181 agatttttgc tgtaataggt actaaaattt tttctattat taccccaata cttgctaatg
      241 aaaggcattg agatttagac gatgaaagac tggagctgga gcaaagagtt gtatacacta
      301 aagttgctga agtattagct gcggcctcga tttcagtttt tgccgttgat ccaggaaccc
      361 ctccttgtcc aagtaatcca aatttttcac agtttgaaac tgaactcaga gtcaataaac
      421 aaattaaata tagagatttt attctcatta tttttctcct tttatctgtg tttttctctc
      481 ccagacattt cagtctgttc tataagaaag cagattttct gctgcacaga tttctttaag
      541 tccagacgaa ttacatatga ggaaaaagtg caagaatatt ttctggtcag gcggtgtttg
      601 ataagtgcac atatttatta gtaagtcaca tattttatta tataattttt gtcaatatac
      661 tcataattga gtattgaact gtattagata attatgagaa ttgaaatcat ggaatatatt
      721 cctgtttttt atgcgtttta ttgagttgta taatatgtat aaatattata ttttttttat
      781 gtaacatgaa ttgggtatgc tgtctgttct ttctctgcac gagatgttcg aaatttcaac
      841 tgaatttttt ttatgaacag tgtttttgca tgttgaggag cacaaaaaga gagtctttat
      901 cggatcgtat ccttaaatga gtgtgaccgt ttatggtttt ccaggaagaa aattcagtaa
      961 aaaagtttat taaaagcact gttttgatct ttattctcag tgaatgatga aaaattaaaa
     1021 ataatgatga aatttatttt ttggattgac ggtctttgca gagaaatttc tccatgcaat
     1081 tatgaaatcc gcttacagga aaaatctggt ttacggaatc atcgatgatg accgtcagtt
     1141 cagttcgact cttgctggac ttctgaagct gaagaataca acccgggaag tgcaccagtt
     1201 tatgtcggct gaagatgcac tgcagtcagg aattctcaaa gacctggatt ttcttttcgt
     1261 ggacagcagg ctggatggga tggatggaat tacttttctt ggacagcggc aggtaaggga
     1321 actcagtatt ccgaaactga ttatatccgg tttcaatgca gagggcagaa tttttgatgc
     1381 aatgaagtac ggtattgcag gttatatgtt caaggaagaa atgcatttgc tgaatcacat
     1441 tctggagaca attttttcgg gaggcgcgtt tctttcaccg tcaattgcat ttcgagttct
     1501 ggaatatttc cgaagggaca ggactc
//
LOCUS       DAOQGJ010001184         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62618_length_1524_cov_3.525528, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001184 DAOQGJ010000000
VERSION     DAOQGJ010001184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62618_length_1524_cov_3.525528"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1524
                     /locus_tag="PK683_16235"
     CDS             <1..>1524
                     /locus_tag="PK683_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNH10047.1"
                     /translation="AIAMNRLGAKSNTGEGGEDEIRYETLPNGDSMRSSIKQVASARF
                     GVTANYLVNADDLQIKMAQGAKPGEGGQLPGHKVDDYIGKLRYSTPGVTLISPPPHHD
                     IYSIEDLKQLIFDLKNVNPRSRVSVKLVSEVGVGTVAAGVAKAHADHILIAGHDGGTG
                     ASPISSIHHAGTPWEIGLAETHQTLVANGLRDRVYLAVDGKLMTGKDVVVGALLGAEE
                     FGFSTSALVTIGCIMMRKCHLNTCPVGVATQDEFLRSKFMGKPEYVVNFMMFIAEEVR
                     EYMAELGFRKFEEMVGQVEKIRFDRPHKHWKARGLDFSKVLHKPKPHFPTDLYKTKEQ
                     KHKLEQQLDNELIRYSRAAIDRGKQVNHETAVTNLNRSVGTMLSGEVSRKYGEKGLPE
                     DTINITFKGSAGQSFGAFVTSGITLKLIGDSNDYVGKGLCGGKLIVKVNPGVKFSPEE
                     NIIIGNTCFYGATKGTAYINGIAGERFSVRNSGATVVVEGIGDHGCEYMTGGRVIVLG
                     "
BASE COUNT          460 a          314 c          395 g          355 t
ORIGIN      
        1 tgcaattgct atgaacagac ttggtgcaaa atcgaatacc ggagagggcg gggaagatga
       61 aatccgttat gaaacacttc ctaacgggga cagtatgaga tcctccatca agcaggtcgc
      121 atccgcgaga ttcggagtca cagcaaatta tctggtcaat gcggatgatc tccagataaa
      181 aatggcgcaa ggcgcgaaac caggagaagg cggacagctg ccagggcaca aagtggatga
      241 ctatatcggc aaacttcgat acagtactcc cggagttaca ctgatttccc ctcctcctca
      301 ccatgatatt tattcgatcg aagacttgaa gcagctgatc tttgacctga agaatgtcaa
      361 tccccgttcc agggtcagcg taaagcttgt atctgaagtc ggcgttggaa cagtcgctgc
      421 cggggtcgca aaagcccacg cagatcatat tctcatagca ggtcatgacg gaggaactgg
      481 tgcaagtccg atatcgtcca tccatcacgc aggaacccct tgggaaatcg gtcttgcaga
      541 aactcatcag acacttgttg caaacggtct tcgggacaga gtttatcttg cagtggacgg
      601 aaagttgatg acaggaaaag acgtagtcgt cggagctctg ctcggtgctg aagaattcgg
      661 attctccaca tctgcacttg tcacgatagg atgcattatg atgagaaaat gtcatctgaa
      721 tacctgtcct gtcggtgtcg ccactcagga tgaattcctt cgttccaagt ttatgggaaa
      781 accggaatat gtagtgaatt ttatgatgtt cattgcagaa gaagtccgtg agtatatggc
      841 ggaacttgga ttcagaaagt ttgaagagat ggtgggtcag gtggagaaaa tccgtttcga
      901 cagacctcat aagcattgga aagcgagagg tttagacttc tccaaggttc ttcataagcc
      961 gaaacctcat ttcccgactg atctttataa gactaaagaa cagaagcata agctggagca
     1021 gcagctggac aatgaactga tccggtattc cagagccgca atcgacagag gaaaacaggt
     1081 gaatcatgaa actgctgtca caaacctgaa cagaagcgtc ggaacaatgc tttcaggaga
     1141 agtctccaga aagtacggag aaaaaggact tcctgaagat acaatcaata ttactttcaa
     1201 gggaagtgcc ggtcagagtt tcggagcatt tgtaacttca gggattacgc tgaagctgat
     1261 cggtgattca aatgactatg taggcaaagg tctatgcggc ggaaaactga tagtaaaagt
     1321 aaatccgggt gtcaaatttt ctccggaaga aaatatcatt atcggaaata cctgcttcta
     1381 cggcgcaaca aaaggaacag catacattaa cggaattgca ggagaaagat tttcagtcag
     1441 gaactccggt gcaacagtgg ttgtggaagg gatcggtgat catggatgcg aatatatgac
     1501 aggaggacgc gtcattgttc tagg
//
LOCUS       DAOQGJ010001185         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62631_length_1524_cov_3.170184, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001185 DAOQGJ010000000
VERSION     DAOQGJ010001185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62631_length_1524_cov_3.170184"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..>1524
                     /locus_tag="PK683_16240"
     CDS             26..>1524
                     /locus_tag="PK683_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10048.1"
                     /translation="MINFETMYFLPSRKKAKNRSERRIFGEREFNGRTELEKLAENLN
                     SLYPDDLMTAYFREKTAAEVQEKIFGDKPVCLVLIGPKGCGKTALLHQAFRNGLRSFL
                     SNYSSDFAEFLFKFAFNIWLLDPERLIAGMRYVGQWERRAEAIFRHLYSQEIINEKIS
                     QFSRSRFRNMILPQEFIRKPDFLYIRNTVSLFYTGMSSDSSLTVADVLFPYLERKNFG
                     FIAEATPEEWSKVRQLNRSFSELFYTVNIAEMEKTDIEKIVIQERLNLEKESADVKIT
                     NDALKYLFLSDARFRTDTVPPKSLIDILTQTVKRHIFTDTAEIRESFKSIFHYNDIIF
                     DREKTLTDESILNFLDSRIVGQNAAKRTAADVIHTVKAELNDPTKPSASLLFIGPTGA
                     GKTELAKAMAEFLFESERYLVRFNMNEYADAESVSRLIGDFKNPEGTLTVQVSRRKAC
                     VLLLDEIEKAHPSVHDLLLQVLGEGRLTNSLGAVTDFKYAVIIMTSNIGA"
BASE COUNT          485 a          309 c          329 g          401 t
ORIGIN      
        1 gttttttgca tctcagagcg acaccgtgat taattttgaa acaatgtatt ttcttccttc
       61 ccgcaaaaag gcaaagaacc gttcagaacg cagaattttc ggcgaaaggg aattcaacgg
      121 aaggacagaa ctcgaaaaac tcgcagaaaa cctcaacagt ctttatcctg atgatttaat
      181 gacggcatat ttccgagaaa aaacggcagc agaagtacag gaaaaaatat tcggtgacaa
      241 gccggtttgc cttgttctga tcggaccaaa aggatgcggc aaaacagccc tgctgcatca
      301 ggctttcaga aacggtctca ggagttttct ttcaaattat tcttccgatt ttgcggaatt
      361 tcttttcaaa tttgcattca atatctggct ccttgatccc gaaagactaa ttgcaggaat
      421 gaggtacgtg ggtcagtggg aaaggagagc tgaggcgata ttcagacacc tgtattcaca
      481 ggaaatcatt aatgaaaaaa tatcacagtt cagcagaagc agattcagga atatgattct
      541 gcctcaggaa ttcatcagaa agcctgactt tctgtacatc aggaatactg tttcactttt
      601 ttacacagga atgagttctg acagcagtct cactgtcgcc gatgttcttt tcccttatct
      661 ggaaaggaaa aatttcggat tcattgcgga agcgactccg gaagaatggt cgaaagtcag
      721 acagctgaac agaagtttct ccgagctttt ttacacagtg aatattgctg aaatggagaa
      781 aaccgatata gaaaagatcg tcatacagga aagactgaat ctcgaaaaag aatctgctga
      841 tgtgaaaatc acaaatgatg ctctgaaata cctttttctt tcggatgcaa gatttcggac
      901 ggacactgtc cctccgaaat cactgatcga cattctgact cagacagtca aaaggcacat
      961 ttttacagat acagcggaaa tcagagagtc cttcaagagc atattccatt ataatgatat
     1021 tatatttgac agagaaaaaa cacttacgga tgaatcaata ctgaattttc tggacagcag
     1081 aatcgtagga cagaatgcag caaaaagaac tgctgcagat gtaatacata cagtaaaggc
     1141 tgaactgaat gatccgacca agccttctgc atctcttctt ttcatcggtc ctaccggtgc
     1201 cggcaagaca gagcttgcca aggcaatggc agaatttctg tttgaaagcg aacgttatct
     1261 tgtccgattc aatatgaatg aatatgctga tgcggaatct gtttccagac tgatcggcga
     1321 tttcaagaat cctgaaggaa ctctgacggt tcaggtcagc cgaagaaagg catgcgttct
     1381 tctcctggat gaaatagaga aagctcatcc ttcagttcat gatctgctcc ttcaggtact
     1441 cggtgaaggc agacttacaa actcactcgg agcagtgacg gattttaaat atgcagtcat
     1501 tataatgaca agcaatatag gtgc
//
LOCUS       DAOQGJ010001186         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62642_length_1524_cov_2.912866, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001186 DAOQGJ010000000
VERSION     DAOQGJ010001186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62642_length_1524_cov_2.912866"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..>1524)
                     /locus_tag="PK683_16245"
     CDS             complement(44..>1524)
                     /locus_tag="PK683_16245"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNH10049.1"
                     /translation="NAQVSALKINAKGECLSETPLAVTQSSSAGDYTLNFAKIGQPVC
                     VRVSPLSGSTVYDEKSSKYIAWKDAKPMETILNESSLVSSAGRKTRSSLHLTPFSKIA
                     VARIAELAKNNSSMENISTLTSRANREITIRFGLSGAVKSKALSDSDYPDLNELGASF
                     ENPDDPTALKMKMILAGISQVAYNTRDSESGTTQSDIDQVIEGFANDGADGLFDGKTS
                     SGSSVQISGIKPLTLTSNSLADNLLPAVSQFISEGGSLSISPDDPKPNTPVTAATVSG
                     SISFNGTAPITTSFSGTSTGTATAVTPSITYPSATLNLVGLSAIPPVNPIVVGTLTSF
                     SISPALPAGMTFSTSTGAIGGTVPFLISNQLYTVTGTGTSGTVSASITIQTVLPGKRI
                     FVTATRAGSWGAVASIDTYCAADANKPAGAGGPYKAMVGYSNGTRRACSVTNCAGVNG
                     AQASDWVFANGTSYYRPDGTFISTATIDGILILPLTNSIVLL"
BASE COUNT          372 a          335 c          389 g          428 t
ORIGIN      
        1 atttgcgcct gtgacgtacg gcacatcggg ctgaagagtg gaatcaaaga agaacgatgg
       61 aatttgtcag aggaaggatc agtattccgt caattgttgc agtcgaaata aaagttccat
      121 ccggtctgta ataagaagtt ccgtttgcaa atacccagtc ggatgcctgt gctccattca
      181 cgcctgcaca gttggttaca gaacaggctc tccttgtccc attgctgtag cctaccatcg
      241 ccttgtacgg tcctcctgct ccggcaggtt tgtttgcatc agcagcacag tatgtatcga
      301 tacttgccac agctccccag cttccggctc ttgtggcagt aacaaaaatt ctttttcccg
      361 gaaggactgt ctgaattgtg atcgatgcag atactgttcc tgaggtgccg gttcctgtca
      421 cagtgtacaa ttgattggaa atcaggaacg ggactgttcc gccgatggct ccggtcgaag
      481 tactgaatgt cattcccgca ggaagggccg gactgatgga gaaactggta agagttccca
      541 caacgatcgg attgactggc ggaatcgcag aaagtccaac cagattcaga gtggcacttg
      601 ggtaagtgat agaaggagtg actgcagtcg cagtcccagt ggatgttccg gaaaatgagg
      661 ttgtgatcgg tgcagttccg ttgaaactga tacttccgga aactgtcgct gcagtcacag
      721 gagtgtttgg tttaggatca tcaggactga tggaaagaga acctccttcg gaaatgaact
      781 ggcttaccgc aggaagaaga ttgtccgcaa gagaattgga tgtcagtgta agaggtttga
      841 ttccgctgat ctgcacggag gaacctgagc ttgtcttccc gtcgaaaagt ccgtccgcac
      901 cgtcatttgc aaatccttcg atgacctggt ctatatcgga ctgagtggtg cccgattcag
      961 aatctcttgt attgtacgcc acctgtgaaa tccctgcaag tatcattttc attttcaatg
     1021 ctgtcggatc atcagggttt tcgaatgatg ccccaagttc attcagatca gggtagtctg
     1081 agtcggagag tgcttttgat ttgacagcac ctgaaagtcc gaaccgaatt gtgatttccc
     1141 ggtttgctct ggacgtcagt gtggagatat tttccatact gctgttgttt ttggcaagtt
     1201 cagcaatcct tgcgactgca attttggaga atggagtgag atgcagactg cttcttgttt
     1261 ttcttcctgc tgaagacaca agggaagatt cattcagaat ggtttccatc ggttttgcat
     1321 ctttccatgc aatatatttt gatgattttt catcataaac tgtggaaccg gaaagaggac
     1381 tgactctgac gcagacaggc tgaccgatct ttgcaaaatt caaagtataa tctcctgcgc
     1441 ttgaggactg agtgactgca agtggtgttt ctgacagaca ttctccctta gcattgattt
     1501 tcagtgcaga tacctgagca tttc
//
LOCUS       DAOQGJ010001187         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62769_length_1522_cov_3.507157, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001187 DAOQGJ010000000
VERSION     DAOQGJ010001187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62769_length_1522_cov_3.507157"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1522
                     /locus_tag="PK683_16250"
     CDS             <1..>1522
                     /locus_tag="PK683_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10050.1"
                     /translation="AIQNAKQQIEKLLPIIAEADSFEKVESEMEELEKQHQSLPSFFA
                     HIKISVLEPEIRTKEEERKRLENLTLEIEEELKNLRENEISLNVQIQNLQESKELDRL
                     NYEMEKLEGIKKNKKKNSDLYNDYITKINYIEPETEDSFYALREKAQKDLQEKDTNLQ
                     VIDEEILRFRMTQKDLSEKRRSIEAELESLRRRKSLIPETNLRTRKILLDHLDLEESK
                     IPFAGELIRVKDKEWEGAAERVLHGFALSLLVPDDLYSGVSTIVNKVNLKSRIVYYRV
                     RPQKQRKDSNIEKPLFDRLEIKSDSEFYEWLENELRESYGYSCAESLDEFQKMQRAVT
                     KEGQIKYNQSRHEKDDRRDILDRSSYVLGWNNKEKIQSMEISLENIVSESASVQEKFQ
                     QAEKGKRRLEENISALKRILEFENYHELNWKKEASEITEIRAKILELKNSSDMLKVLN
                     VQLDETRNRIREKDSFKSEKNKETGNIQSYLASLSQQKMEQNRYLEMMTEDERNIYF"
BASE COUNT          576 a          257 c          360 g          329 t
ORIGIN      
        1 gccatccaga atgcaaaaca gcagatcgaa aaacttcttc ctatcattgc tgaggctgac
       61 agttttgaaa aagtagagtc ggaaatggag gagctggaaa aacagcatca gagtctgcca
      121 tccttctttg ctcatataaa aatctctgtt cttgaaccgg agatcagaac aaaggaagaa
      181 gaaaggaaga gactggaaaa tctgactctg gaaattgaag aggagctgaa aaatcttcga
      241 gagaatgaaa tcagtctgaa tgtgcagata cagaatcttc aggagtccaa ggaactggat
      301 cggctgaact atgaaatgga aaaactggaa ggaataaaaa agaacaagaa aaagaattca
      361 gatttatata atgattatat cactaaaata aattatattg aaccggagac agaggacagt
      421 ttttatgcac tccgggaaaa agcacagaag gatctgcagg aaaaagacac gaatcttcag
      481 gttatcgacg aagaaatcct ccgattcaga atgactcaga aggatctctc tgaaaaaaga
      541 aggtccattg aggctgaact ggaatcttta cgcagaagaa aatcactgat tcccgagaca
      601 aatctcagaa caaggaaaat acttctggat catctggatc tggaagaatc aaagattcca
      661 ttcgcggggg aattgatcag agtaaaggac aaagaatggg aaggcgcagc agaaagagtt
      721 cttcacggtt ttgcactgag tcttcttgtc ccggacgatc tgtattctgg agtcagcaca
      781 atcgtaaata aggtaaatct gaaaagcaga attgtatatt acagagtgcg tcctcagaag
      841 cagagaaagg attccaacat agagaagcct ctgttcgaca gactggaaat caagtcagac
      901 agtgagtttt atgaatggct ggaaaatgag cttcgggaaa gttacggata ttcctgtgcg
      961 gaatcactgg atgaatttca aaagatgcag agagccgtca caaaggaagg acagatcaaa
     1021 tacaatcagt ccagacatga aaaggacgac agacgggaca ttctggacag atcttcttat
     1081 gtgctgggat ggaacaataa agaaaaaata caaagtatgg aaatcagcct ggaaaatatt
     1141 gtatccgaat ctgcatcggt tcaggaaaaa ttccagcagg cggagaaagg aaaaagacgt
     1201 ctggaagaaa atatttctgc actgaaaaga attctggagt ttgaaaatta ccacgagctg
     1261 aactggaaga aggaagcatc cgaaatcact gagatcagag caaaaattct ggaactgaaa
     1321 aactcatctg atatgctgaa agttctgaat gttcagctgg atgaaaccag aaacaggatc
     1381 agagaaaagg attcattcaa atcggaaaag aacaaggaga ctggaaatat tcagtcctat
     1441 ctggccagtc tctctcagca gaaaatggaa cagaaccgtt acttggaaat gatgacggag
     1501 gacgaaagga atatctattt tc
//
LOCUS       DAOQGJ010001188         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62775_length_1522_cov_3.290389, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001188 DAOQGJ010000000
VERSION     DAOQGJ010001188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62775_length_1522_cov_3.290389"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PK683_16255"
     CDS             <1..527
                     /locus_tag="PK683_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10051.1"
                     /translation="GEMTSKPVLFEYAIKRYPPFSNESKDIDDIPGNSAKTVGNVFRN
                     ILEEKLLEYRNFKEYAVVKERNSAMYKGLYDYKVKINISFSQGFSFYYIPALLSLSMI
                     PAKAFYSDYEAEIKIFGRDDSLLKKYRFEEEVSYWYAIYYFGLAGDHLNSVNAFQYII
                     DKILFKIQEDRIIR"
     gene            678..>1522
                     /locus_tag="PK683_16260"
     CDS             678..>1522
                     /locus_tag="PK683_16260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH10052.1"
                     /translation="MKELISHIPAPICIYDAETLQISSVNEAFTELYGYSEEDAVSMS
                     VYDLLPPSERETLRNQFSETDKDRLISGEWHHIRKDGSQITVLIHSRSILYEGKKHIT
                     AVITDVTEIKKTQRELVSEKNRLRTLIQSIPDPVWVKDTEGVYLICNSMVERLFGVQE
                     SEIIGKTDYNLTDKKTADTFRENDRQAMETKTASVNEEWIRSADTGTLSLFETVKTPM
                     YDDSGNLLGIMGIARDITERRKNQHDLRERAKENVCLYNIFSLTENSNSPVSEVLQKA
                     AEKAG"
BASE COUNT          522 a          297 c          316 g          387 t
ORIGIN      
        1 aaggggaaat gacaagcaag cctgttcttt ttgaatatgc aataaaaaga taccctccgt
       61 tctcgaatga atcaaaggac atagacgata ttcccggcaa ttctgccaag acagtcggca
      121 atgtatttcg aaatattctg gaggaaaaac ttttagaata cagaaacttt aaggaatatg
      181 cagtcgtcaa agagcgaaat tctgcaatgt acaaaggact ctatgattac aaagtaaaaa
      241 taaacatttc cttcagtcag ggattttctt tctattatat ccccgctctg ctctccctgt
      301 caatgattcc tgcaaaagca ttctattccg attatgaagc agagataaaa atattcggca
      361 gagacgactc cctattgaag aaatacagat tcgaagaaga agtctcttat tggtatgcaa
      421 tatactattt cggactggca ggcgatcatt taaattcagt caatgcattt cagtacatca
      481 ttgataaaat tcttttcaaa attcaggaag acagaatcat ccgataaaca atatatccgg
      541 atacggatca tttttgattc cgctgatgta ccggcaggga aacagtctgc agaaactgcc
      601 gctttcaatt cttaagacgg agaaggtttt cgcttgacaa aaccgatttt ttccgcaact
      661 ttaaacagga ggctgcaatg aaagaactga tttcacatat tccggctccg atctgcatct
      721 atgacgcaga gactcttcag atcagttctg taaatgaagc atttacggaa ctgtacgggt
      781 attcggaaga agatgcagta tcaatgtctg tttatgatct gcttccgccg tcggaaagag
      841 aaacactgag aaatcagttt tcggaaactg acaaagaccg tctgatcagc ggagaatggc
      901 atcatatccg aaaggacgga tcacagataa ctgttctgat tcattcccga agcatcctgt
      961 atgaagggaa aaaacacatt acagcagtca tcaccgatgt gacggaaata aaaaaaacac
     1021 agagagaact tgtctccgag aagaacaggc tgcgcaccct gattcagtct attccggatc
     1081 cggtctgggt caaggataca gaaggagtat acctgatctg caattcaatg gtggagcgac
     1141 ttttcggagt acaagaatcg gaaataatag gaaaaactga ctataattta actgataaaa
     1201 agactgccga tacattcagg gaaaatgaca gacaggctat ggaaacaaag actgcctctg
     1261 taaatgaaga atggatcaga tcagcagata ccggcacact gagtcttttt gaaaccgtaa
     1321 aaacacctat gtacgatgat tcaggaaatc ttttaggcat tatgggaatt gccagagaca
     1381 tcactgaacg gcgcaagaat cagcatgatc ttcgggaaag agccaaggaa aatgtatgtc
     1441 tatataatat attttcacta actgaaaatt ccaattctcc ggtttcggaa gttcttcaaa
     1501 aagccgcaga aaaagcaggg ga
//
LOCUS       DAOQGJ010001189         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62856_length_1521_cov_3.135744, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001189 DAOQGJ010000000
VERSION     DAOQGJ010001189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62856_length_1521_cov_3.135744"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1290)
                     /locus_tag="PK683_16265"
     CDS             complement(136..1290)
                     /locus_tag="PK683_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10053.1"
                     /translation="MALAGVILYPNFAERQLQLVVDPDFVSLSKEEQEKNLKKFSEKW
                     SQDYNAKGAYRIEPDPSAGIPAGGKFIVTGRYITTAKINQISQENIQLFQESQNKFLP
                     TIVERWFNGKSLTIKLGLDLQGGMRVVLKGDFDSYVSKLKDLYAKEIADLNATILDPA
                     KSKDEKEKARTRLDNLNQSFELNKNRKLLELEKAKLIIDNRLTNQNLTEPQVRIQNDQ
                     DSIEVALPGVSNSTQILEILQNTETVEYRLEEPSDGTRRGKYESMIDNAEQDLVNQGK
                     KDQTDIVQYQKLIQDRRGKKALDDFLASLEDKYKIPKDQYKVFAFWGRGAKPNSPMLP
                     RRFVVLEKAIRDAHSATALRAVGQQKKPAAPGFFVAIHLPGAPLLDDQAI"
BASE COUNT          379 a          358 c          333 g          451 t
ORIGIN      
        1 accggccagg agtcgaatct cggcgaccgg cccgctgagc tttggagcgt ggcgcctacc
       61 gtgctgtcgg ggcaacggcc gacaggcgaa ccggtaaatc acatcctcac ggcgatcaac
      121 gtcacggacg tacagttaga tggcttgatc atccaggagg ggggcgcccg gaagatgaat
      181 ggcgacgaag aaacccgggg cggcgggctt tttttgttgg ccgacggccc tcaacgcggt
      241 tgcgctgtgc gcatcacgaa ttgccttttc caggacaaca aatcttctag gaagcatcgg
      301 gctgttcggc ttcgctcctc ttccccagaa cgcaaatact ttatactgat cttttggaat
      361 tttgtattta tcttctaatg atgcaaggaa atcatccagg gccttctttc ctcttctgtc
      421 ctgaatcagt ttctggtact gaacgatatc ggtctgatct tttttgcctt gatttacgag
      481 atcctgctct gcattgtcaa tcatagactc atacttccct cttcttgttc cgtcagaagg
      541 ctcttcgagt ctgtattcaa cagtttctgt gttctgcagg atttcaagaa tctgagtgga
      601 attggatact ccagggagcg ccacttcaat ggaatcctgg tcattctgta ttctcacctg
      661 aggttcagtc aggttctgat ttgtcagcct gttgtcgatg atcagttttg ctttttcgag
      721 ttcgagcagt tttctgtttt tattcagttc aaaggactga ttcagattat cgagtctggt
      781 ccgtgccttt tctttttcat ccttggattt tgcaggatca agaatggtcg cattgagatc
      841 agcaatttcc ttagcgtaaa gatccttgag tttggataca taactgtcaa aatcaccctt
      901 caggacaact ctcatcccgc cctgaaggtc aagacccagt ttgatagtaa gagattttcc
      961 gttaaaccat ctctccacaa tcgtaggcag aaacttattc tgtgactcct gaaagagctg
     1021 aatattttcc tgagaaatct gattgatctt tgcagttgta atgtatcttc cagtgacaat
     1081 gaacttcccg ccggcaggaa tgcctgcaga aggatccggt tcaatcctgt acgcaccctt
     1141 ggcattatag tcctgactcc atttttcaga gaactttttc aggttttttt cctgctcctc
     1201 tttggaaaga gaaacaaagt cagggtcaac tacaagctgg agctgccttt ctgcaaaatt
     1261 gggatataaa atcacaccgg ctaacgccaa aatgataaga ggtaaaacaa tccactggga
     1321 agatttcaaa actgtatcct gttccttttt tatttatcgt taaatttact gaatatccgc
     1381 cctgaagacg aaccttgaga agagcgggaa accccgtttc tcaatctgta aagtgcaaaa
     1441 ataaccacta tgccgacaac caaaaaaacc tttttgtatt cggagaaaaa ctgcacaaga
     1501 gtatttttct cgacaggaat t
//
LOCUS       DAOQGJ010001190         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62872_length_1521_cov_2.577080, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001190 DAOQGJ010000000
VERSION     DAOQGJ010001190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62872_length_1521_cov_2.577080"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1436)
                     /locus_tag="PK683_16270"
                     /pseudo
     CDS             complement(153..1436)
                     /locus_tag="PK683_16270"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2219 family protein"
BASE COUNT          443 a          314 c          305 g          459 t
ORIGIN      
        1 aattcaataa attataaaat atatttgatt acgaataaat aaaatttcat gaaactgtct
       61 ttttctttca gcatatgttt cagcagaaat tcaattctta ctccgattcc ggaatgcctg
      121 atcattcgtg catcaaatcc gattttcata tttcagaacc tgtaagaaag ctgaaagccg
      181 aaccactgat gatactgtgc attgtatgca gcaggcctga aatctgtcgt actgagagtg
      241 taggatatat tcccataaaa atttcctttt ccgaatccaa tgcctgtctg aactgaaccc
      301 tggaccggca ttttttcagc gtagtttctt ctgtctttcc tgaattcgcc ggaagcggcg
      361 agaaatgaga gtaccagatt ctgatgcgtt tcattccccg gaaagatatt tttcagcatt
      421 tcctttgttc cgacaaatct tgcggtttca taatcaattt tattatataa gaagacatta
      481 tatagaagaa gaaattttgt ttcattgtcg agagttgtcc agtcgttgaa aagagaggaa
      541 tagaaggcct tgtattcctg cgcaccgttc aggtatcctc cgttgaagac tgtattgtac
      601 accagatgat cctgaaccgg atctgcagtg ttttccggtc tgtagaacat tttaaaatat
      661 ccgaatcggg aaagatcatt tgaatacttt tcccttgcaa aatcatcatt aaagaatctg
      721 ttcaggaaga ctccgtttcg ttctttcctg cccaggctct gcagagttcc gtcatatgcc
      781 tgaaatttaa tgctgggaat aaagaaaaaa taaaattccg attctgaact ttcagaccgc
      841 ctgcccggaa gcataggcga gtccattgag tgaccgttca gatatgtatc ctgtctcaca
      901 gttccgtaca taaaaagcat actgagtcca atgctgctgt ccagatttcc tgcggaggct
      961 gtctgcctga aggaaatatt tctgttcagt ttctgtgcac cggaaagaga agtgttcaaa
     1021 attcctgtgt caggcatctg atgttcccag ccttcaggtt caagtgaaat aaagccgctg
     1081 cctgtcataa aaagcggaag agaatgaact cctttctgta ctgattctgc atagctctgc
     1141 tttccaagag ttcctattct tgtttcagca aaaacagcag aactctcatt gatgaatgaa
     1201 tgtgagctgc tcaaatagag cactcctgcg tgaggcctgt cgccatatac aggcattttc
     1261 tgaaacagat tgtcgggagt gtagattttc tgattcagag acagtgtgac ataagatttc
     1321 tgaccttctc cctttttgaa aaattttcca tacagaaact cggaaggaga aaacatctgt
     1381 tctctgccgt attccagcct ctgcccgctg gtatagtatc tgtctgaaag caggagatca
     1441 ttttcaaaaa aggctctgaa agttgaatct gcaaaaacag aaaaaggcta aaaaaagaaa
     1501 gcaggtgctt ttcggcagaa t
//
LOCUS       DAOQGJ010001191         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_62996_length_1519_cov_3.383197, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001191 DAOQGJ010000000
VERSION     DAOQGJ010001191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62996_length_1519_cov_3.383197"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PK683_16275"
     CDS             <1..754
                     /locus_tag="PK683_16275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH10054.1"
                     /translation="TLRNYFAQANIGALISMIKVVQESIPPAEDGSVLLDRELAGEIF
                     DSADVGAKILESLNQYVHLAADECSFEDCTVSDFFELLYSAVADLKEKTAEKKTSIVL
                     GTGLYLNTNSRMHLDKKRIRVVISELLINALKFSKKEDKVYVIPVATSTQIGFQIINT
                     AYQFKKGVTGIPLEYQKKIFEPFFKMMEYSDTFFFRQEITSGLGLGLTLVKSIIEKHG
                     AGVTITNITDHFENKDAPTVKVMAEVTFMREL"
BASE COUNT          480 a          302 c          325 g          412 t
ORIGIN      
        1 gactctcaga aattattttg cgcaggcaaa tataggcgca cttatcagta tgattaaagt
       61 cgtacaggaa tcaattccgc cagcagaaga cgggtcagtt cttctggaca gagaacttgc
      121 gggagaaata ttcgacagtg cagatgtcgg agccaaaatt cttgaatcac tgaatcagta
      181 tgtgcactta gctgcagacg aatgcagttt tgaagactgc actgtttccg atttttttga
      241 attactgtac agtgctgttg cagatctgaa agagaaaact gcagaaaaga agacatccat
      301 tgttcttgga accggtctgt atctgaatac aaattcaaga atgcatctgg ataaaaaaag
      361 aattcgggtt gttatcagtg aacttctgat caatgcactg aagttttcca aaaaagaaga
      421 caaggtatat gtcatcccgg tggctacaag cacacagata ggatttcaaa taataaatac
      481 tgcctaccag ttcaaaaaag gcgttacagg aattccactg gaatatcaga aaaagatatt
      541 tgaacctttt ttcaaaatga tggaatactc agacacattt tttttccgac aggaaattac
      601 aagcggactg ggactgggtc tgaccctggt caagagcatc attgaaaaac acggagccgg
      661 agtgacaatc acaaatatca ccgatcattt tgagaacaag gatgctccca ctgtcaaagt
      721 tatggctgaa gtcaccttta tgagggaatt atgatcgttc ccgaaaaatc cctgcaaact
      781 tacatccgca tttagaacgc cccatccata ttaatttaca aaatattaga aaaatctgat
      841 ctgtcactca ccttacgcct acggctgcag gtggggcggt tttggcaggg agcctaccgg
      901 cggtttcgca aactatgaag ctgccgcaaa ctatgaagct gccgcaaact atgaagctgc
      961 taagggcttt gccctcgcca aaacgatggc tgccaagctt tatgaagatg gctgcctgtt
     1021 tggaagctgg ctcggcgact ctgaaactgc ctaaaatccc gcaagcgtgg aagttgctgt
     1081 tttttctgat tcattagatt ttcctaatgc cttctgaaaa ttacagaatc tagatagcat
     1141 attgtatttg cgtaaggtga gttaataaaa aaattagagt tgttgaaaaa ttataagagc
     1201 attgtcataa tcgaacactg cacagccggt gaaacaagtt ctgcgacggc aaaaaaaagg
     1261 acgaatttct tcgtccggag tgttttaatg aaaggaaaaa aaatgttaaa aaatgatgac
     1321 agaatgggag cagatcgctc ccgaatggaa agagcttatg cagccctctc cttcaatact
     1381 tttgcacctg ctcccttagg tgattttttt gtgaactttc ttgcagtctc atcgggaaaa
     1441 gaatatgcaa tgattctttt cactactgaa ctgaactgac tcaggaaact tccgttattg
     1501 tagtactgac cgtatttct
//
LOCUS       DAOQGJ010001192         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63025_length_1519_cov_2.363388, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001192 DAOQGJ010000000
VERSION     DAOQGJ010001192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63025_length_1519_cov_2.363388"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1046
                     /locus_tag="PK683_16280"
     CDS             174..1046
                     /locus_tag="PK683_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10055.1"
                     /translation="MRKILMRILFILFLSSFLGCSTWRQGVLPPFFSWSEKGDQLASV
                     QVKYDEKDSWNPMMGTTDKRSYKTDVKIFKLESGKLSEKPVEEKSLDSWVLPGSFYYS
                     DAGDFFAVLGSPDSGFGTESKIFSYFKSFSSPAEVLWKFKDTLNLEKAVPSPDKSKAV
                     LILSSSSQDGTLKFSAELLDLKSKSSVKTDLPGWFDSPEHRAVWEKDSSSVYFRIKDK
                     VYSLSEKTAFRPVSQFPECFWPETSFGDSVGADGIQAEYSMESQSVKISSEKVKDYKP
                     FEKIRRTSSLKNCR"
     gene            complement(1055..>1519)
                     /locus_tag="PK683_16285"
     CDS             complement(1055..>1519)
                     /locus_tag="PK683_16285"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNH10056.1"
                     /translation="LNLARQFLKREVEKTYYAWLVQTPKNSDGRIDAPIGRHPELRLK
                     MCVSPRGRKAVTHYKIIQTISSKKHRHFSLAEIKIETGRTHQIRVHFQHIGAPIVGDM
                     LYSRSGAEFSKYGLLLFSGKLSFTHPGTGKKMKFELPLPENFILFEKEAPLR"
BASE COUNT          437 a          296 c          334 g          452 t
ORIGIN      
        1 caaaatgaga tttgcacggc tcaagaagat atcgaaggac aatttcaata tgtttgaagt
       61 ggcaatgctc tgtcacaagc ttgcagctga ttacaggaaa ctggcaagac tcaacagctg
      121 gaaggaaata gaagcgatgt ttgactgagc ctcttcctac gaaatataaa atcatgagaa
      181 aaattttaat gcgcattcta ttcattcttt tcctgtcttc attcctgggg tgcagtacgt
      241 ggagacaggg tgttcttcct cctttttttt catggtcgga gaaaggggac cagctggctt
      301 ctgttcaggt caagtatgat gaaaaagatt cctggaatcc gatgatgggg acaacagaca
      361 agaggtcata caaaacagat gtaaaaatct ttaaactgga atccggaaaa ctttctgaga
      421 aacccgtcga agagaaaagt ctggattcct gggttcttcc aggaagtttt tattattcag
      481 atgcagggga cttttttgca gttctcggtt cacccgattc cggatttggc acagagtcta
      541 aaattttttc ttattttaaa agcttttcat cacctgcaga agtattatgg aaattcaaag
      601 atacactgaa tcttgaaaag gcagtcccat cccctgataa aagcaaggct gtactgatcc
      661 tttcttcgag ttcacaggat ggtacattga aattttcggc tgagcttctg gatctaaagt
      721 caaagtcatc cgtcaaaaca gatcttccgg gctggttcga ttcacctgaa cacagggcag
      781 tctgggaaaa ggattcatct tctgtctatt tcagaataaa ggataaggtt tacagcctgt
      841 cagaaaaaac ggcattcaga ccggtttcac agtttccgga atgcttttgg cctgaaactt
      901 cttttggaga cagtgtcggt gctgatggta ttcaggcgga atattcaatg gaatctcagt
      961 ctgttaaaat cagctctgaa aaagtaaagg attataaacc gtttgaaaaa atcagacgga
     1021 cttcttcact gaaaaactgt cgctaaacga agatttatct gagcggggct tccttttcaa
     1081 aaagaataaa attttcgggc agagggagtt caaatttcat ttttttccct gtgccaggat
     1141 gagtaaaact gagttttcct gaaaacagaa gaaggccgta cttgctgaac tctgcaccgc
     1201 ttctggaata cagcatatct cctacgatcg gtgcaccaat atgctgaaaa tgaacacgta
     1261 tctgatgagt tcttccagtt tcgattttga tttctgcaag tgaaaagtgc ctgtgttttt
     1321 tggaggaaat ggtctgaatg attttataat gggtgactgc ctttctgcct cttggactga
     1381 cgcacatctt cagccggagt tcaggatgcc ttccaattgg agcatcaatt ctgccgtctg
     1441 agtttttagg agtctgtaca agccatgcat agtaagtctt ttccacttct cttttcagaa
     1501 actgtcttgc cagattcaa
//
LOCUS       DAOQGJ010001193         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63027_length_1519_cov_2.282104, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001193 DAOQGJ010000000
VERSION     DAOQGJ010001193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63027_length_1519_cov_2.282104"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /gene="atpB"
                     /locus_tag="PK683_16290"
     CDS             <1..534
                     /gene="atpB"
                     /locus_tag="PK683_16290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNH10057.1"
                     /translation="SLFFFILFCNLFGLIPPVGEIVVALSGGGHDAVAGKIWSGITVT
                     GDISVTVTLAGITLIMIYLTGFMYQGPLFPIYSVPNGVPGWTYILMFPLEFVVSPLAK
                     AFALTVRLLANMTAGHVVILALLGFIFEYQSYFIVPVSILASVAIYMLEIFVAFLQAF
                     IFTLLTSLFVGSSMHRH"
     gene            566..871
                     /gene="atpE"
                     /locus_tag="PK683_16295"
     CDS             566..871
                     /gene="atpE"
                     /locus_tag="PK683_16295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNH10058.1"
                     /translation="MDFGLGYIGVGIGAGLAILGAGLGIGRIGGQAVEGMSRQPEAAG
                     KIQTAMLIAAAFIEGAALFALVIAFSAGGALNKSLPDSLTKAAVTAPAASTEAPAKK"
     gene            884..1402
                     /locus_tag="PK683_16300"
     CDS             884..1402
                     /locus_tag="PK683_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNH10059.1"
                     /translation="MVLAAGGLNLLQVNPGLLIWTIVTFLIVVGILKKFAWGPIVHAL
                     EERAEKIHGDIEKAEKLRADAEGLLSDYKSKLASAGEEAQSVLNEAKKDAANLKNKMI
                     QEANNEISGMKSQALKDIELSKVKAIQEIQQNIVELSVSIASKILEKQLKPEDHANFV
                     KSEINNLKTMKG"
BASE COUNT          414 a          309 c          363 g          433 t
ORIGIN      
        1 tctcttttct tcttcatcct tttctgtaat cttttcgggc tgattcctcc tgtaggagaa
       61 atcgtagttg cactttccgg gggcggtcac gatgcagttg cagggaaaat ctggagcggt
      121 attaccgtca caggtgatat ttccgtcaca gtgactctcg cagggattac cctcattatg
      181 atttatctga ctggttttat gtatcaggga ccgcttttcc cgatatactc tgtaccgaac
      241 ggggttcccg gctggacata catactgatg tttccattgg agtttgttgt atcaccgctt
      301 gcaaaagcat tcgcccttac agtgcgtctt ttggcgaata tgacagcagg tcacgtcgtc
      361 atactcgctc ttctaggatt tattttcgaa tatcagtctt atttcatcgt tcctgtatcg
      421 atcctggcat ctgtagcaat atatatgctg gagatctttg tagcattctt acaggcgttc
      481 atttttactc tgctgacctc acttttcgtg ggttcaagca tgcataggca ctaattttta
      541 gtaaatttat ttaagaggat aaactatgga tttcggttta ggatacattg gtgtagggat
      601 cggagctggg cttgcaattc tgggtgcagg acttggtatt ggcagaatcg gcggacaggc
      661 tgttgaaggt atgagccgtc agcctgaggc tgctggaaaa attcagactg caatgctgat
      721 tgcagcagcg ttcattgaag gtgctgctct tttcgcactg gtgattgctt tctcggctgg
      781 cggtgctttg aacaaaagtc tcccagattc tctgactaaa gcagcagtta ctgctcctgc
      841 agcttctaca gaagctccag ctaagaagta agagggttcc aaattggtac tggcagcagg
      901 cggtttaaat cttctacagg taaatccggg tcttctgatc tggacaatag tgactttcct
      961 gattgttgtg ggcattttga aaaaatttgc ctggggtccg attgtccatg cattggaaga
     1021 aagagccgaa aagattcatg gcgatatcga aaaggctgag aaacttcgtg cagacgcaga
     1081 gggactttta tctgactaca agagcaaact tgcatctgcc ggcgaagaag cgcagtctgt
     1141 attaaatgaa gcaaaaaaag acgcagcgaa tctcaaaaac aaaatgattc aggaagctaa
     1201 taacgaaata tccggaatga agtcccaggc tttgaaggat attgagcttt ccaaagtgaa
     1261 agcaatacag gaaatccagc agaatatcgt ggaactttcc gtatccatcg cttccaaaat
     1321 tcttgaaaag cagctcaagc cagaggatca cgcaaatttt gttaaatctg agataaacaa
     1381 cctcaagaca atgaaagggt gatatgctcg actctcatat agcaaaagtt tatgcaaagg
     1441 cgcttctgga aataggagcg gaaaagaaca acgcagacat tctcgaagaa gaactgagag
     1501 aaactgcaag ttctttgag
//
LOCUS       DAOQGJ010001194         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63167_length_1517_cov_2.449384, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001194 DAOQGJ010000000
VERSION     DAOQGJ010001194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63167_length_1517_cov_2.449384"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PK683_16305"
     CDS             <1..563
                     /locus_tag="PK683_16305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNH10060.1"
                     /translation="SAIGFYGMYEEGPEFTEDSDSGNDFLSLTCREWEESANAGAEKI
                     RTVNARIGIVLSEKGGALSQMLTPFRLFAGGPVGSGKQFMSWIHVTDAVNAFRFIIQN
                     SNIKGPVNLTSPLPSSNAEFSRVLGKVLHRPSWLPVPETALRILFGDGAEIVLKGQKV
                     LPKRLLDSGFLFQFSNLESAMKNLLG"
     gene            623..1375
                     /locus_tag="PK683_16310"
     CDS             623..1375
                     /locus_tag="PK683_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10061.1"
                     /translation="MRAILTNPYTKKEDILNLGPGQEESCALLGLPERQAYEKMEPKL
                     YEYLDSFFSDPSKIAKFGEKRVLAIYRLGNKVIQRGLCSLLRIQYKYKAEIQKNELLP
                     FFFGLHSMNIKGLLFLADNYPLQWSEMDFRKEENEEIFEDSILLFYLLEEQNPAVNLL
                     DRMKGETRPLDFIFREEIKKWKDSAKPVDTDSSEMMESYKSRKLKESTMVIFTQEDKK
                     RLYLEVYEKIAMHWRYLVDRPLQPLKFDFDIR"
BASE COUNT          474 a          289 c          320 g          434 t
ORIGIN      
        1 cttcagcaat aggcttttac ggtatgtatg aggaagggcc tgaatttaca gaggattcag
       61 attcgggaaa tgattttctt tctttgacct gcagagaatg ggaagagtct gcaaatgcag
      121 gtgcagagaa aatcagaaca gtcaacgcga gaataggaat tgtactgtct gaaaaaggag
      181 gggcactgtc tcagatgctc actcctttcc ggctcttcgc aggcggaccc gtaggttccg
      241 gaaaacagtt catgagctgg attcatgtga cagatgcagt aaatgctttc agattcatca
      301 ttcagaattc aaatataaaa ggtcccgtca atctgacctc cccccttcct tcctctaatg
      361 cagaattcag cagagtatta ggaaaagtcc ttcacagacc ttcctggctc cctgttcctg
      421 agacagcatt aagaatcctc tttggtgacg gagcagaaat tgttctaaaa ggacagaaag
      481 tccttccgaa acggcttctg gattcagggt ttctttttca attctccaat ctggaatctg
      541 caatgaaaaa tcttttagga tgaaaaaaat aatttgacct gatgaataat tatgaattag
      601 aatttctctt ggaaatattc tgatgagggc gattcttaca aatccatata ctaaaaagga
      661 agacatcctt aatctgggtc ctggccagga agaatcctgt gctcttcttg gtcttcctga
      721 aagacaggcc tatgagaaaa tggagcctaa actgtatgaa tacctggaca gttttttcag
      781 tgatccttca aaaattgcaa agttcgggga gaagagagtt cttgccattt acagattagg
      841 taataaggtc attcagagag gtctctgcag cctgcttcgt attcagtata aatacaaggc
      901 tgagattcag aaaaatgaac tgctcccgtt tttcttcggt cttcattcca tgaatataaa
      961 aggcctgctt ttcctggctg acaactatcc tctgcagtgg tccgaaatgg attttaggaa
     1021 ggaagaaaac gaggagatat ttgaagattc catccttctt ttttatctgc tcgaagaaca
     1081 gaaccctgcc gtaaatctgc tggacagaat gaaaggtgag acaaggccct tggactttat
     1141 cttcagagaa gagatcaaga aatggaagga cagtgcaaag ccagtagata cagattcatc
     1201 cgaaatgatg gagtcctaca agagcagaaa actgaaagaa agtacaatgg ttatttttac
     1261 ccaggaagat aaaaaaagac tttatcttga agtgtatgaa aaaattgcaa tgcactggag
     1321 atatttagtc gaccgtcctc ttcagccttt aaaatttgat tttgatatcc gataaccctg
     1381 atgttaaaag gtaaaacctt ggtcgatttc acagactgta atcctgcagt caccgatacc
     1441 taattttttc cttgtaattt ttcagaaaat ttgaattctt agaaattcgt aactaactgg
     1501 agttcctatg aaaattt
//
LOCUS       DAOQGJ010001195         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63213_length_1516_cov_3.433265, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001195 DAOQGJ010000000
VERSION     DAOQGJ010001195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63213_length_1516_cov_3.433265"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1318
                     /gene="aroC"
                     /locus_tag="PK683_16315"
     CDS             203..1318
                     /gene="aroC"
                     /locus_tag="PK683_16315"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNH10062.1"
                     /translation="MPSTWGKVFKVTTFGESHGVSVGVVVEGVPGNLEFPLEEIQKDL
                     TRRKPGQNLLTTPRKEDDEIRVVSGVFEGRTIGSPITLIVDNKNAIGRDYEKFRHIYR
                     PSHADYTYDIKYGFRNHEGGGRASVRETIGRVAAGAIARIILEKELGLETIAWVDSIG
                     TVDADPEIIPKSKSEVDRNDVRCPDEKAAVLMEEKIREVRKVGDSIGGTVRAVVRNVP
                     PGLGDPVYDKLDGDLGRAVLSIPACKGFEIGSGFSGSRFKGSEHNDEYYVEKETGRIR
                     TRTNRSGGLQGGISNGEEILIRAAFKPTATISIEQETVSDKPEDTVLKAGGRHDPCVL
                     PRAVPIVEAAINLVLVDAYFYQRALHPEWFEKFRRKH"
     gene            1315..>1516
                     /locus_tag="PK683_16320"
     CDS             1315..>1516
                     /locus_tag="PK683_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10063.1"
                     /translation="MMKKIHYIAVMISALPLISGKVIYKGESWIGPKTVIRKEAPLNT
                     GTVQRETPNVIKEMDKKPASNQT"
BASE COUNT          503 a          293 c          385 g          335 t
ORIGIN      
        1 ttgagaatag taccatcctg gggtttttac atttgtataa atacaattca tgaacagaat
       61 gagcataggc agtgtttttt taatcataga gcagtctttt atgttcctga aaaacaggtc
      121 aagctgcaaa tgatgaccgt ttacagaaag cctcactcag ttaatcaatt gaatttctga
      181 gtgaatgtaa aaaaatgacc ctatgccgtc aacttgggga aaagtattta aggtaacaac
      241 gttcggagaa tctcacggag tttcagtcgg ggttgtagtg gaaggggttc ccggaaatct
      301 ggaatttcca ttggaagaaa ttcagaagga tctgaccaga agaaagccgg gccagaatct
      361 gcttacaact cccagaaaag aggacgacga gatcagagtt gtatccggtg tattcgaagg
      421 cagaacaatc ggcagtccca ttactctcat tgtagacaac aagaatgcaa tcggaagaga
      481 ctatgaaaaa ttcaggcata tctacaggcc ttctcatgcc gattatacct acgatataaa
      541 atacggattc agaaatcacg agggcggcgg aagagcctcg gtcagagaga ctataggaag
      601 agttgctgcg ggagccattg ccagaataat actggagaaa gaacttggac tcgaaacaat
      661 tgcctgggta gattcgatcg gaacggtgga tgccgatccg gaaatcatac cgaagtcaaa
      721 atcggaagtg gacagaaatg acgtcaggtg tcctgatgaa aaggctgcag ttcttatgga
      781 agaaaaaatc cgtgaggtca gaaaagtagg agattccatc gggggaacag tcagagcagt
      841 tgtcagaaat gtccctcctg gactcggaga tcctgtatat gataaactgg acggagatct
      901 cggaagagcc gtcttatcca ttcccgcctg caaaggattt gaaatcggtt ccggtttttc
      961 aggaagcaga ttcaagggaa gcgagcacaa cgatgaatac tatgtggaaa aggaaacagg
     1021 aagaatcagg acaagaacca accgttccgg cggccttcag ggaggaattt ccaacggtga
     1081 ggaaatactg attcgggcag cattcaaacc cacagccacg atttccatag agcaggaaac
     1141 agtcagcgac aagccggaag acacagtgct caaggcggga ggaagacatg atccctgcgt
     1201 tcttcccaga gctgtgccca tagttgaggc agccataaat cttgtacttg ttgatgcata
     1261 tttctaccag agagcactgc atccggaatg gtttgagaaa ttcaggagaa aacattgatg
     1321 aaaaaaatac attatattgc agtaatgata agtgcacttc cgctgatttc cggaaaagtg
     1381 atatacaagg gcgaaagctg gattggaccg aaaacggtaa tcagaaaaga agctcctctg
     1441 aatacaggaa cagtgcagag agaaactccg aatgtaatca aggagatgga taaaaaacct
     1501 gcttcgaacc agactg
//
LOCUS       DAOQGJ010001196         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63292_length_1515_cov_3.469863, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001196 DAOQGJ010000000
VERSION     DAOQGJ010001196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63292_length_1515_cov_3.469863"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PK683_16325"
     CDS             <1..1145
                     /locus_tag="PK683_16325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH10064.1"
                     /translation="RDSSFDILIADPFTVSENPISILKELNESAPNLQIIIFSAESNY
                     KKIIESLNEIRNSHYLAKPSSAEELKNALEDAFNTVRVRRKSEDFSLSERKFYSEMIR
                     IFDWKKEITKRKIDYFTKDLIHQVNIGLLHGSGLGSLLGSLSLLFSKAEISEDGTKAV
                     IPKFLYDAVNEDYEQSRELMKGLSMAQNIIMENESVDTSFHPDLIYSLVKNCAAALNP
                     MFQLKNQKAVFGSFPAFSQDTQVIMNSEKLGFVFSELLINAMKYSKNEDIIYILFYKR
                     DDNLEIKILNPAYEFSPGSPGISGIYETLVFEPFFRMIDSVDDDYSMETVRFGLGLTL
                     AKKILELHGGDIIIFNVGNNLNQKEKTDVCVSVRLPLKTQKEGTEQ"
     gene            1142..1507
                     /locus_tag="PK683_16330"
     CDS             1142..1507
                     /locus_tag="PK683_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH10065.1"
                     /translation="MKKILIVDDSPSVIRVVKMALAMEGYDITSAANAEQAEEILKGN
                     SFDFGIFDVNMPGKNGIELVRTVMGLPNGKDMKILILTTESSDELKKKGREAGAKGWL
                     IKPFNHLDLLTVLKKLGEI"
BASE COUNT          511 a          273 c          323 g          408 t
ORIGIN      
        1 tccgagactc aagtttcgac atactgattg cagatccatt caccgtttca gaaaatccta
       61 tttctattct gaaagaactg aacgaatcgg ctccaaatct gcagattatc attttcagtg
      121 ccgaatcaaa ttataaaaaa attatagaat cattaaatga gatcagaaat tcgcattatc
      181 ttgcaaagcc gtcctctgca gaagaactta aaaatgcact ggaagatgca ttcaatacag
      241 tcagagtcag aagaaaatct gaagacttct ctctttctga aagaaaattt tacagtgaaa
      301 tgatcaggat tttcgactgg aagaaggaaa ttacaaaaag gaaaatcgac tattttacaa
      361 aggatctgat acatcaggta aatataggac tgctgcacgg atcaggtctg ggttctcttc
      421 tcggatctct gagtctttta ttttcaaaag cggaaatttc cgaagacggc acaaaggcag
      481 tgattccaaa atttctttat gatgcagtga atgaagatta tgaacagagc agagaactta
      541 tgaagggact ctctatggct cagaacatca ttatggaaaa tgaatcagtg gatacttcct
      601 tccatccgga tctgatttac tcgcttgtga aaaactgtgc agccgccctg aatccaatgt
      661 ttcagctgaa aaatcagaaa gcagtcttcg gatccttccc ggccttcagt caggacactc
      721 aggtgattat gaattcggaa aaactgggat ttgtattttc agaactgctt ataaatgcga
      781 tgaagtattc aaaaaatgaa gacatcattt atattctttt ttataaaaga gacgataatc
      841 tggaaataaa aatactcaat ccagcctatg aattttctcc cggcagtccc ggcatttcgg
      901 ggatttatga aacacttgtg tttgaaccgt ttttcagaat gattgattcc gttgacgatg
      961 actactcgat ggagactgta agattcggtc ttggactgac cctcgcaaaa aagattctgg
     1021 agctccacgg aggagacatc attattttta atgtgggcaa taatttgaac cagaaagaaa
     1081 aaacggatgt ctgtgtttca gtcagacttc cgctgaaaac acagaaagaa ggaacagagc
     1141 aatgaaaaaa atactgatag tggatgattc accgagtgtg atcagagtgg taaaaatggc
     1201 tcttgcaatg gaaggatatg acatcacaag cgcagcaaat gccgaacagg cagaagagat
     1261 actgaaaggc aattctttcg attttgggat ttttgatgtg aatatgcccg gaaaaaacgg
     1321 aattgagctg gtcagaacgg taatgggatt gccgaacggc aaggacatga aaattctaat
     1381 tctaactacc gaatccagtg atgagctgaa aaaaaaggga agggaggctg gtgcaaaggg
     1441 ctggctcatc aaacccttca atcatcttga tcttctgact gttttgaaaa aactgggaga
     1501 aatctgaatt ttgcc
//
LOCUS       DAOQGJ010001197         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63298_length_1515_cov_3.380137, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001197 DAOQGJ010000000
VERSION     DAOQGJ010001197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63298_length_1515_cov_3.380137"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PK683_16335"
     CDS             complement(<1..738)
                     /locus_tag="PK683_16335"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNH10066.1"
                     /translation="MRYIISFFLASAFYVQALEVRLKDIAKVGGSRDNQITGYGIVTG
                     LAHTGDTKNSVTSESMKNYLKNQGLTTSGNVLTARNIASVMVTAEIPAYAKTGDRIDV
                     TVSSIGDAKSLEGGVLLQSPLKAANGETIAVASGTLSFGGKDTRPVNMSRTGTKTVGI
                     ISGGAIVEKDIQSGFLVLAEETDGKGTVKARYLKTSVILENQDYATLDSVNKSILQSF
                     QAEKVQTKLVSPVEIEVFIPEKADSASI"
     gene            complement(735..>1515)
                     /locus_tag="PK683_16340"
     CDS             complement(735..>1515)
                     /locus_tag="PK683_16340"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNH10067.1"
                     /translation="GMKTIKILIIFSVLGLSAQENPSAKSAPAKEIPAKTPPAKETAV
                     SIPVSGRSLWTDGSPYSSASGAKTFKKGMIVRVIIRDGIKGDYTFESAKDETVVIRSA
                     PDKKIVPELMGYTSDRTIARKGNGKEKSTAKVLGTIAVTVVDKDENGNLLLEGSREAN
                     FEKGRSSLTVTGKINPEDLRDGNTVYSDYVADLVIVYDASPVKTELKDPDLKMKPAKL
                     PNGQPQINRDDGTPMEKAEMNETEKQKVILKNIKRLLGESE"
BASE COUNT          335 a          379 c          308 g          493 t
ORIGIN      
        1 tatggatgca ctgtctgctt tctcaggaat gaatacctct atctccacag gagaaacaag
       61 ctttgtctgc accttttctg cctgaaagct ctgaagaatt gacttattca ctgagtcaag
      121 ggtggcatag tcctgattct ccaagatcac gcttgtcttg agatatcttg cctttacagt
      181 tcccttgccg tccgtttcct ctgcaagaac cagaaatcct gactggatgt ctttctcaac
      241 gatcgcacct ccggagatga ttccgaccgt cttggttccg gttctgctca tattgaccgg
      301 tctcgtatcc tttcctccga aagaaagagt ccctgaagca accgcaattg tttccccgtt
      361 tgcagctttc agaggagact gaagaagaac acctccttca agagattttg catctccgat
      421 cgaagaaaca gtcacatcga tcctgtctcc tgtctttgca tatgcaggga tttccgcagt
      481 caccatcact gatgcaatgt ttcttgcagt aagaacattt ccggatgtgg tcagtccctg
      541 attcttgaga taatttttca tgctttcaga tgtaactgaa ttttttgtat cccctgtatg
      601 cgcaagtcca gtgactatcc cgtatcctgt aatctgattg tctctggaac cgccgacctt
      661 tgcaatgtcc ttgagcctga cttcgagtgc ctgaacatag aatgcagatg ccagaaaaaa
      721 acttataata tatctcattc gctttctcca agaagccttt tgatgttctt gagtatgacc
      781 ttctgttttt ccgtctcgtt catctccgcc ttttccatcg gagtaccgtc atctcggttg
      841 atctgaggct gtccgttcgg cagttttgca ggtttcatct tcagatcagg atctttcagt
      901 tcagtcttca cgggagacgc atcgtaaaca atgacaagat ctgccacata atcactgtag
      961 actgtgtttc cgtcccgaag atcctcagga ttgatctttc ctgtgacagt gagggaactt
     1021 cttccttttt caaaatttgc ctctctgctc ccttccagaa gaagatttcc gttttcatcc
     1081 ttgtctacaa cagtcactgc gatcgttccc agcacctttg cagtcgattt ttccttcccg
     1141 ttgccttttc ttgcaattgt tctgtcggaa gtgtagccca tcagttcagg aacaattttt
     1201 ttatccggtg cagaacggat gactacagtt tcatcctttg cagactcaaa tgtatagtct
     1261 cccttgattc cgtctctgat gatcactcgg acgatcattc cttttttgaa ggtttttgct
     1321 cctgatgctg atgaataagg acttccgtct gtccagagac ttctgccgct gacaggtatg
     1381 gaaacagccg tttcttttgc ggggggagtt ttagcgggaa tctcctttgc cggtgcagat
     1441 ttggcagatg ggttttcctg tgcagaaagc cccagtacag agaatataat caatattttt
     1501 atcgttttca ttcca
//
LOCUS       DAOQGJ010001198         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63465_length_1513_cov_3.628258, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001198 DAOQGJ010000000
VERSION     DAOQGJ010001198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63465_length_1513_cov_3.628258"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PK683_16345"
     CDS             <1..1074
                     /locus_tag="PK683_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10068.1"
                     /translation="DNSITFYRKEAEEKVEDKKKEEEFKLGPDWICSFCESLNSQVDA
                     KCKGCGATREDSEKNYFDELKKREEKQKKKDERDGKTEEPPKEIPKAGKWWKIGAGAV
                     GLLGILYSWGTCSTNIEYKVKSVSWIRAVEVERYQTENRSDWKGHLKGDSIQVLSEKR
                     EVKGHEKRQIGSRMESYSDTERVQSGTKKECSTSYKSTGSGASKKTTSCKDVPTYTNR
                     SVTRTRSVPVYQDFPIYDTKVYYRSKAYSTLGYAVKKGKDNNPEWPKPKLESGEDGKK
                     DRIGEKAESLQVTIQRTKGDKGPDEETFTTTEKLFSEYDPESVHKFSVANVGGISYKN
                     DEKKLDKEEFEKKFRPILSRAIH"
     gene            1262..>1513
                     /locus_tag="PK683_16350"
     CDS             1262..>1513
                     /locus_tag="PK683_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10069.1"
                     /translation="MIFLLALIFLFLAVPFFKTDSRILTSGLIAVLSASVLIFTCFPK
                     AGKIRLSFKEKNFFLISSFIYILYFIILLYFDFKNFYLAD"
BASE COUNT          533 a          292 c          324 g          364 t
ORIGIN      
        1 gacaacagca tcacattcta ccgaaaggaa gctgaagaaa aggtagagga taaaaaaaag
       61 gaagaagagt ttaagcttgg gccggactgg atctgctcat tctgcgaatc tctgaactct
      121 caggtggatg caaaatgcaa aggctgcggt gcaaccagag aagacagcga aaaaaattat
      181 tttgatgaac tgaaaaaaag agaagaaaag cagaagaaaa aggatgaaag agacggaaaa
      241 accgaagaac ctccaaaaga aattcctaaa gcaggaaaat ggtggaagat cggtgcaggg
      301 gcagtcgggc ttttaggaat actttactcc tggggaacct gttccacgaa catagagtat
      361 aaggttaaat cagtgtcctg gatcagagca gtcgaggtag agagatacca gacagaaaac
      421 cgttcagact ggaaaggaca tctgaaagga gattcaatac aggttctttc tgaaaaaaga
      481 gaagtcaaag gacatgaaaa aagacagata ggctcccgaa tggaatccta ttctgatact
      541 gaaagggttc agagcggaac aaaaaaagaa tgctccacaa gttacaaatc caccggttcc
      601 ggagcaagca agaagaccac ttcctgcaaa gatgtaccga cctatacgaa cagaagtgta
      661 actagaacaa gatcagttcc cgtttatcag gattttccga tctatgatac taaggtttat
      721 tacagatcaa aggcttacag cactctcgga tatgccgtaa agaagggaaa ggacaataat
      781 ccggaatggc ctaaaccgaa gcttgaatcg ggcgaggacg gaaaaaaaga cagaatcggt
      841 gaaaaggcag aatctctgca ggtgacaata cagagaacaa agggagacaa aggacctgac
      901 gaagaaactt tcacaaccac tgaaaaactt ttctcagaat atgatcctga atcagtgcat
      961 aaattttcag tcgcaaatgt aggcggaatc agctacaaaa acgatgaaaa aaaacttgat
     1021 aaggaagagt ttgaaaagaa attcagacct attctttcaa gagccattca ctgaaggaaa
     1081 aatatatccg gactgatctg acagtacccg ctgaaccagt ccggactgcc ttccaatttt
     1141 tttttgagag agaactgatc atttcttttc agtgcagaaa tgcagaaccg tctttccagt
     1201 cccaattaaa ctgcagtcac actgaacaga atatcagcct tgatttgccc ctcaggaaca
     1261 gatgattttt ctactggccc tcatatttct ttttcttgct gttccttttt tcaagacaga
     1321 cagcaggatt ctgacttcag gtctgattgc ggttctgtcc gcctccgttt tgattttcac
     1381 ttgttttcca aaagcaggga aaatccgact ctcatttaag gaaaaaaatt tctttctgat
     1441 ttcctcattc atatatattc tctattttat tatactttta tattttgatt ttaagaattt
     1501 ctaccttgcc gac
//
LOCUS       DAOQGJ010001199         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63573_length_1512_cov_3.105010, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001199 DAOQGJ010000000
VERSION     DAOQGJ010001199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63573_length_1512_cov_3.105010"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PK683_16355"
     CDS             <1..792
                     /locus_tag="PK683_16355"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNH10070.1"
                     /translation="ATGLLWQKCSYGASGPTCATGTQTSLNFANASSYCSSLTAGGKT
                     WRIPTIRELHSIHYLANNPRIDTSKFGATQAGDYWASDTAVFNSSAAWTVSFYDGTTL
                     TTPQTNLRYLRCVTGTEKKTVSNFTDLGDGTVKDHSTGLYWQKCTNGQNSLDCSGTGT
                     AAADWASAVSYCNTLNLAGKTWRLPNKNELVSILDYQKTTFPLTNTDVFPNMPNNQLY
                     WSSTTNNVTGFFNEALVLDFGSFSKGRMNIESKGWSFDYVKCVAD"
     gene            complement(954..>1512)
                     /locus_tag="PK683_16360"
     CDS             complement(954..>1512)
                     /locus_tag="PK683_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10071.1"
                     /translation="SVYPKKMFPDLSMLLHYFQYAENKPELENTSRILDRMYPDRLSL
                     EENELSLRQQWNEFIQDKKGVESGGAVYTDIADLLQGQSPFIWSGHWVFDGVVLDRYH
                     DGEIDFNSYYASHFHSKENSERFEQFEKFMKSIGMNREKTWTLFSPSGERIWKAVAVL
                     CHDGNTHWILLEGENEFLDICSSGS"
BASE COUNT          466 a          371 c          308 g          367 t
ORIGIN      
        1 gctaccggac ttctctggca gaaatgctca tacggtgcaa gcggcccgac ctgtgcaaca
       61 ggaactcaga catctctcaa ttttgcaaat gcatcctctt actgttcttc actgacagca
      121 ggaggaaaga cctggagaat tcctacgatc agagaactgc attccatcca ctatcttgcg
      181 aataatccca gaatagacac atcaaaattt ggagccacac aggccggcga ctactgggca
      241 agcgacaccg cagtattcaa ctcctctgcg gcctggacag tatcatttta tgacggaaca
      301 acactgacta cacctcagac caacctgaga tatctgagat gcgtcacagg gacagaaaag
      361 aagacagtaa gcaattttac agatttggga gacggaacag taaaggatca ttccacagga
      421 ctttactggc agaaatgcac aaacggacag aacagtctcg actgctccgg caccggaact
      481 gcagctgcag actgggcctc agcagtcagc tactgcaaca cactgaacct ggcaggaaaa
      541 acctggagac tgccgaataa aaatgaactg gtatccatac tggactatca gaaaacaact
      601 tttccactta caaacactga cgtctttcca aatatgccca acaatcagct gtattggtct
      661 tccactacaa acaatgtcac gggatttttc aatgaggcgc ttgtcctgga tttcggatcg
      721 ttctccaaag gacggatgaa catagagtcc aaaggatggt ctttcgacta tgtaaaatgc
      781 gtcgccgact gattgaatat aatataatga tcataaaaac agcaagtcca tccggcaaag
      841 atcttctgaa ccggacggcc cgactgtcaa atacaggaaa atttttcaga tgaggcctga
      901 aacagatatg cttgaaaata tgaattttca aaaaatcatt atgatatatc taatcagctt
      961 ccggaggaac agatatccag gaactcattt tctccttcca gcagaatcca atgagtgttt
     1021 ccatcgtgac agagaaccgc gacagccttc cagatccttt ctccagaagg actgaacagt
     1081 gtccaggtct tttcccgatt cattccaatc gacttcataa acttctcgaa ctgttcaaaa
     1141 cgctccgagt tttcctttga atgaaaatgg gaagcataat aactgttgaa gtcgatttcc
     1201 ccatcgtggt acctgtcgag aacaacaccg tcaaacaccc aatgcccgct ccagataaac
     1261 gggctctgtc cctgcagaag atcagcgatg tcagtataca ctgcgccccc gctttctact
     1321 ccttttttat cttggatgaa ttcattccac tgctgtctta aagaaagctc attctcttca
     1381 agagacagcc tgtccggata catacggtca agaatgcggg aggtattttc aagttcaggt
     1441 ttattctcag catactgaaa ataatggaga agcattgaaa gatcaggaaa catttttttg
     1501 ggatatacgg ac
//
LOCUS       DAOQGJ010001200         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63755_length_1510_cov_2.748454, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001200 DAOQGJ010000000
VERSION     DAOQGJ010001200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63755_length_1510_cov_2.748454"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK683_16365"
     CDS             <1..196
                     /locus_tag="PK683_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10072.1"
                     /translation="ITAEIFIQRQRRIWKTRDSLQEAIKNGDNSEVEWIISEADLNTY
                     GGVGEMSRYQIFRPKKVEIR"
     gene            225..500
                     /locus_tag="PK683_16370"
     CDS             225..500
                     /locus_tag="PK683_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNH10073.1"
                     /translation="MRRRKNYDPNFRARVALEAAKGNKTLSELSGEYKVHANLIVKWK
                     KEMLQKLPTLFQKADRHEIEEAMEKNDELYKEIGKLKMELDYYKKNF"
     gene            502..891
                     /locus_tag="PK683_16375"
     CDS             502..891
                     /locus_tag="PK683_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10074.1"
                     /translation="MSISDRKGLIEDDEPFSVREQCRLLSVNRSSLYYIQKPESSLNL
                     KIMRMIERQHTDDPARYGQKKMAEYLSRKLKIPVSRNCVQRPMRYGYMYLNAVAQRWT
                     GIPDMSFRGRYQTVWKQISAFLLSCAH"
     gene            801..1241
                     /locus_tag="PK683_16380"
     CDS             801..1241
                     /locus_tag="PK683_16380"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNH10075.1"
                     /translation="MDWYSRYVISWEISNSLEADFCILALLRALKKGRPEIFNTDQGS
                     QFTSSEYTGTLESSDILVSMDGKGISLDNIFIERLWRTLKYEDIYKRSYETPQELYEG
                     LNSYFSFYNRRRLHQALSYSCPEEVWLKSLTQNRASAIIAGGQK"
BASE COUNT          518 a          267 c          342 g          383 t
ORIGIN      
        1 aattactgcg gagattttca ttcaaagaca aaggagaatt tggaagacaa gagacagctt
       61 gcaggaagca ataaaaaacg gagataacag cgaagttgaa tggattattt cagaggccga
      121 ccttaatacg tatggaggag tcggagaaat gagccggtac caaattttta gaccaaaaaa
      181 agttgaaata aggtgataaa aagcgatcat atggaggaca gaatattaga cgcagaaaga
      241 attatgaccc aaatttcaga gcaagggtgg cattggaagc agccaaggga aacaagacct
      301 tgagcgagct ttcaggagaa tacaaagttc atgcaaacct gatagtgaaa tggaagaagg
      361 agatgcttca gaaacttccg acactttttc agaaagcaga ccgtcatgaa attgaagaag
      421 cgatggaaaa gaatgacgag ctgtacaagg aaataggaaa gcttaagatg gagctggatt
      481 attataaaaa aaacttctga tatgagtatt tctgacagaa agggactgat tgaagacgat
      541 gagccctttt ctgtcagaga gcagtgccgc cttttaagcg taaacagatc atcattatat
      601 tatattcaaa agccggaaag ttcactgaat ctgaagatta tgagaatgat cgaacgtcag
      661 cacacagatg atcctgcaag atacggacag aagaaaatgg ctgaatatct ttcccgaaaa
      721 ctgaagattc cggtgagcag aaactgtgtt cagagaccga tgagatatgg gtatatgtat
      781 ctcaatgctg tagcacagcg atggactggt attccagata tgtcatttcg tgggagatat
      841 caaacagtct ggaagcagat ttctgcattc ttgctctcct gcgcgcactg aaaaagggga
      901 ggccggaaat attcaatacc gaccaaggca gccagtttac atcttcggaa tataccggaa
      961 cacttgagag cagcgacata cttgtcagta tggacggaaa gggaataagt ctcgacaaca
     1021 tattcattga aagactctgg cgcactctaa aatatgagga tatttataaa aggtcttatg
     1081 aaactccgca ggagctgtat gagggtctga acagttattt cagtttctac aacagacgga
     1141 gacttcatca ggcactcagt tattcctgtc ctgaagaagt ctggctgaaa agcttgactc
     1201 aaaacagagc atctgcaatt attgcaggtg ggcaaaagta ggaaaaagaa atggaggctc
     1261 ttaaaatgtc tcccttcttt ttctgaaaat cccctgtttc ggtaataaag tttactgaaa
     1321 ttcaataagt tttttatcac ctactttttc ttaaaaagtg gtctaaacaa ttgggtatac
     1381 ccccctatgg ctaatcttta atgtcgggct ggttccattt gaattgattt catcgcaagt
     1441 cttttactcc ttacctgtaa tacaaaaatt gcttgcacaa ttttacactc cgtatcaagc
     1501 taagtacagg
//
LOCUS       DAOQGJ010001201         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63790_length_1510_cov_1.969072, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001201 DAOQGJ010000000
VERSION     DAOQGJ010001201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63790_length_1510_cov_1.969072"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1510)
                     /locus_tag="PK683_16385"
     CDS             complement(<1..>1510)
                     /locus_tag="PK683_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10076.1"
                     /translation="TLDSSNFRRGLSFSIKAAEDDNVANEIVQIEIAAEGYQPLAFAL
                     EIKDNDVQRIILSKDRDDVQEGKKKTFEVRLNIKPEQTVSVQLENSDPSAVSISPAVL
                     KFEPSNYNIPQTVTVEALEDADSVSETSFISVGADNLEKSVYTVKTLDNDKTLLFSLP
                     SLSVNEGSVQQVNVSLSEPPEQETVIYFSSSRPSSIQVNPASVTVNPYNWNLPIILDV
                     SALQDANYISEAAVISASSVLHQTASLNVSSVDDEVQAILVSGATTVTEGSNGIIKVR
                     LAQPPTGTISVNLSSSYSKLQIANPVLYFTPLNYSTDQNVTLVGQQDADKDSSVVSIT
                     ASTPGITSAVYPVIFLDNDTGIQITPSAPSIREGSSGSMQVVLNGDPGIPRTVNLSSS
                     LPSLSLGSVILSFDSSNWNIPQTVSLLAVEDIDHNSDIPSVSGSGTGLISTSSSITVF
                     DNDTRAVITGSSSVYEGGSILLNVQLSGNPINPVSVTATSSNTSASTVSPGTL"
BASE COUNT          431 a          337 c          315 g          427 t
ORIGIN      
        1 tgagagttcc cggactgacc gttgaagcac ttgtattgga acttgtcgca gtgactgaaa
       61 caggattgat tggatttccg gaaagctgga cattcagcag aatgctcccg ccttcatata
      121 ccgaactgga gcctgtaatt accgcccgag tatcattgtc aaatacagtt atggaactgc
      181 tcgtgctgat cagaccggtt cctgatcctg aaacagaagg gatatctgaa ttatggtcaa
      241 tgtcttccac tgccagcaag ctgaccgtct gaggaatatt ccagttggac gaatcaaaac
      301 tcaaaatgac tgatcctaaa ctcagagacg gcagactgga agaaaggttt actgtcctcg
      361 ggattcctgg atctccgtta agaacaactt gcatacttcc tgaactgcct tctctgatcg
      421 aaggagcaga tggtgtaatc tgtatcccag tatcattatc taaaaatatt actggatata
      481 ctgcagaagt tattccagga gttgaggctg tgatgctgac gacagaagaa tccttatccg
      541 catcctgctg accgaccaag gtgacattct gatcagtgga atagtttaat ggcgtaaaat
      601 aaagaaccgg atttgcaatc tgaagtttac tgtacgagga actcagattt actgatattg
      661 ttccagtcgg aggctgtgcc agtctgacct ttataatccc gtttgatcct tctgttacag
      721 tcgtcgctcc ggaaaccaaa atagcctgaa cttcatcatc cactgaagat acattcaaac
      781 ttgcggtctg atgcagaact gaacttgcag aaatgactgc tgcctctgaa atataattcg
      841 catcctgcaa agcagaaaca tccagtataa ttggaagatt ccaattataa ggatttaccg
      901 taacgcttgc cggattcacc tgaatgctcg aaggacggct ggaagaaaaa taaatcacag
      961 tttcctgctc aggtggttca gagagagaaa cattgacctg ctgaaccgat ccttcattta
     1021 ctgagagact gggcagagaa aatagaagtg ttttatcatt atccagagtt ttgactgtat
     1081 atacagactt ctccagattg tccgctccca ctgagataaa ggaagtctcc gaaacactgt
     1141 cagcatcctc caaagcttcg acagtgaccg tttgcgggat attgtaattg gaaggttcaa
     1201 atttgagaac tgcaggtgaa atgctgactg ctgaagggtc agaattttcc aattgtacac
     1261 tgactgtctg ttcgggctta atattcaatc tgacttcaaa tgtcttcttt tttccttcct
     1321 gaacatcatc cctatccttt gacaaaataa tcctctgaac atcattgtcc ttgatttcca
     1381 gtgcaaaagc caacggctga tatccctctg ccgctatttc aatctgtaca atctcatttg
     1441 caacattgtc gtcctctgct gccttgattg aaaaactcag tcctcttctg aaatttgaag
     1501 aatccaatgt
//
LOCUS       DAOQGJ010001202         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_63927_length_1508_cov_2.870613, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001202 DAOQGJ010000000
VERSION     DAOQGJ010001202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63927_length_1508_cov_2.870613"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PK683_16390"
     CDS             <1..583
                     /locus_tag="PK683_16390"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNH10077.1"
                     /translation="WRDMRVSSITDLILMKIFIIILALFFSSLQAGTSILDKEAEFTA
                     ALKVKKVEGKVKNFTVSPFTLKRGKNSEFSITPFSIVIPVKELKTGDPNRDSNMQEAL
                     GYPAEKEITLNIQRIQPSETGAYRIFFRMKIKGKEKELESSASVKEESGRIVVSGKFS
                     VFLHDFNIDPPTLLFMTVQDEVFCRYRFSIQAE"
     gene            1039..1389
                     /locus_tag="PK683_16395"
     CDS             1039..1389
                     /locus_tag="PK683_16395"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HNH10078.1"
                     /translation="MKFLRFAAFLLLFSVSLHSQKQVSKESNGFVFSKLHFIDHFTYV
                     LIKGEVLNQSGRYYTDAYFTVKLYDDNNDLLGVSLVLISHFGPGKKKTFDATTDTVDI
                     KMIKKYEIVFENEN"
BASE COUNT          459 a          274 c          312 g          463 t
ORIGIN      
        1 ttggcgagat atgcgagtaa gttctatcac tgatttaatt ttaatgaaaa tatttatcat
       61 tattctggca ttatttttca gttcgctcca ggcaggaact tccattctgg ataaggaagc
      121 ggaatttact gcagctctca aagtcaaaaa agtggaaggc aaggtaaaga attttacagt
      181 atctcccttt acgctcaaaa gaggaaaaaa ttcggaattc agcattactc ccttttccat
      241 tgtgattcct gtaaaagaac tgaagactgg tgatccgaac agggactcca atatgcagga
      301 agccttggga tatccggcag aaaaggaaat tacattgaat attcagagaa tccagccgtc
      361 agaaaccgga gcctacagga tattctttcg tatgaaaatc aaaggaaaag aaaaagaact
      421 ggaatcctct gcttctgtca aggaagaatc aggaagaatt gttgtttccg gaaagttttc
      481 tgtctttttg cacgatttta atatagatcc tccgacactc ctttttatga ctgttcagga
      541 tgaagttttc tgcagatacc ggttttcaat tcaggctgaa taataaggac atcagatttt
      601 tcaaggccta taatttcgaa attcaccttg gtggattaga tattttataa ttatttagag
      661 aagaataaaa tattcaaaag ctttccagta aataactcat cttgctcctg cggtctgggg
      721 caggaaaacc tgctgacagg ttgaaggatc aaaggcttgg aagcatatgc cgcccttgtc
      781 aacgttatga agcttcccgg aagcattttt tgcggtcgtc tcgaaaaact gacctcagac
      841 catccccgtc gatgcttcat ctgaaactaa aggcacaaat cgagcgaaaa attgtaatac
      901 tgagtgaaat tgtgctggct tttcctgaat aattctgcat acttgatgta tgagaattga
      961 tgaaaaactg cgaatatgca tgattcagtc cttttccatg gagacagaca gttctgttta
     1021 tctgtctgcg gcattcgtat gaaattcctc cgttttgcag cttttcttct gttattttca
     1081 gtctctttgc attcccaaaa acaggtatct aaggaatcaa acggttttgt cttcagtaag
     1141 cttcatttta ttgaccattt tacttacgtt ctcatcaaag gcgaggttct gaaccagtcc
     1201 ggccggtatt atacggatgc ctactttaca gtcaaacttt acgatgacaa caatgatctt
     1261 ctgggagtca gtctggttct gatcagccat tttggaccgg gtaaaaaaaa gacatttgat
     1321 gcaacaacgg atacagtaga tataaaaatg attaaaaaat atgaaattgt atttgagaac
     1381 gaaaactgaa tataaggggt ttgcgatgaa gaaacatctg ataagtattc tttttttact
     1441 gttttcatcc tgtctgggtg taagtgtaaa gggcggggag atgggacttc tgtggagacc
     1501 tcttactg
//
LOCUS       DAOQGJ010001203         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64044_length_1507_cov_2.130854, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001203 DAOQGJ010000000
VERSION     DAOQGJ010001203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64044_length_1507_cov_2.130854"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK683_16400"
     CDS             complement(<1..345)
                     /locus_tag="PK683_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10079.1"
                     /translation="MGEAISFFFWVLMFVLGASVVWMQKYLVSRKNLDRSFRIFLLFV
                     GIGFVSMAGFVFSDIKNELYFLHSSASEKKEEKSFQFEDRKNILLIESVSDSVFSLNN
                     LFYSGKNSENRTF"
     gene            complement(391..1080)
                     /locus_tag="PK683_16405"
     CDS             complement(391..1080)
                     /locus_tag="PK683_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10080.1"
                     /translation="MKILIQILLLSFSTGCSAGLLEYTVGYKIFENTSRGKHKAEISE
                     PGLYLSGGNLPRTDQNYKKEYYDSLKEALMISIQNSGRFNSAEWKKENEYEPDSVQLK
                     IFFSALEEMKVGYYPYNPSLFFLFFPLRQDYEENELRLFWPTFFPGLYPLLGLQFPFL
                     AKSGTVRLSLNCKITYKGKVLKQIEIQKQDSYSLMFYGVFRTALPERISILLFERALE
                     ELTLELADLEL"
     gene            complement(1077..>1507)
                     /locus_tag="PK683_16410"
     CDS             complement(1077..>1507)
                     /locus_tag="PK683_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10081.1"
                     /translation="VHIYPFIKENIPVYIPIYLGRTARTEVIQSSVNTVYNPVYSALH
                     PGSDLPVINLTAEALPSFYAGAGLGGKIFLPYGLFLGMEFGYAAIYQPQLHYRSSLSF
                     FNKAQVSIPELALLQEQVKLAHPAADSVKMNYGIILGASF"
BASE COUNT          466 a          302 c          295 g          444 t
ORIGIN      
        1 gaatgttctg ttttcagaat tctttccgga ataaaataaa ttatttaatg agaagacgga
       61 atcagaaact gattcaatca aaaggatatt tttcctgtct tcgaattgga aagatttttc
      121 ttcctttttt tcagatgcag aactgtggag aaaataaagt tcatttttaa tgtctgaaaa
      181 tacgaatcca gccatactga caaatccgat cccgacaaaa aggagaaaaa tcctgaatga
      241 tctgtccaga ttcttcctgg agacaagata tttctgcatc caaaccacgg atgcccccag
      301 aacgaacatc agaacccaga agaaaaatga gattgcttca cccataattg cagattcttt
      361 tcagtcattg ccggggcaag aagaattcct ttacagttca agatcagcca gttcgagtgt
      421 aagctcttcc aatgctcttt caaaaagcag aatggagatt ctttcaggca gtgctgttct
      481 aaaaactccg tagaacatca gggaataact gtcctgtttc tgaatttcaa tctgtttcaa
      541 aactttgcct ttgtaggtta ttttgcagtt taacgaaagc ctgactgtac ctgacttcgc
      601 aaggaaggga aactgcagtc caaggagcgg ataaagtcca gggaaaaaag taggccagaa
      661 cagccgcagt tcattctctt cgtagtcctg ccgcaaagga aaaaacaaaa aaaacagact
      721 tggattatac ggataataac ctactttcat ttcttccagg gcggagaaaa aaatcttcag
      781 ctgcactgaa tcaggttcat attcattctc ttttttccat tctgcagaat tgaatcttcc
      841 ggaattctgt atgctgatca taagtgcttc tttcagggaa tcgtaatatt cctttttata
      901 gttttgatca gttcttggaa gatttcctcc ggaaagatac agtccaggct cggaaatctc
      961 tgctttatgt tttcctctgg aagtattttc aaatatttta tatcctaccg tatattccag
     1021 cagtccagca ctgcagccgg tactgaaact gaggagaagg atttgaatca ggattttcaa
     1081 aagcttgctc cgagtattat gccatagttc attttcactg aatcagcggc agggtgagca
     1141 agtttgacct gttcctgcag taatgcaagt tcaggaatgc tgacctgtgc cttattaaaa
     1201 aaagacagac tgcttctgta atgcagctga ggctgataaa ttgcagcata tccgaattcc
     1261 attcctaaaa acagtccata tggaagaaat atttttccgc ccagtcctgc acctgcatag
     1321 aaggaaggca gtgcttcagc ggtaaggttt attacaggaa gatctgagcc gggatgcaga
     1381 gccgaatata ccggattata aactgtattc acagaacttt gtatcacttc tgttcttgca
     1441 gttcttccca aatatatcgg gatatataca ggaatattct ccttaatgaa gggatatata
     1501 tgaacaa
//
LOCUS       DAOQGJ010001204         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64246_length_1504_cov_2.652864, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001204 DAOQGJ010000000
VERSION     DAOQGJ010001204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64246_length_1504_cov_2.652864"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..392)
                     /locus_tag="PK683_16415"
     CDS             complement(123..392)
                     /locus_tag="PK683_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10082.1"
                     /translation="MNLITILLLILIPAAAYPLYYSFHKNSSYKKKTNTDEPGNAGKS
                     DEKKNKELKKNAQVIEEKLQIFDAMIDQLDKELSQLEKEVDKMDK"
     gene            complement(389..1084)
                     /locus_tag="PK683_16420"
     CDS             complement(389..1084)
                     /locus_tag="PK683_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10083.1"
                     /translation="MYELKNIALNIAGEADSPPEKAVRLHDFVRDRIRFGLTHKLDFA
                     GAEETMELGYGTSSTKGLLLMKLLSEADINSQMHFVTVKKDFYRHCFPDVVYSLMPET
                     VTHCYLEINVDGTYIPTDSYSVERKLFLEARKVLEGIGEKCGFGIHSEGSCDWNGKDP
                     CFSQFSCEEQLVEDHGAFKDPFEYYMHYDSYKHKFLGIRYSDIMKLIGSMAGGNFEIW
                     INSKMNQFRSSHS"
     gene            complement(1090..>1504)
                     /locus_tag="PK683_16425"
     CDS             complement(1090..>1504)
                     /locus_tag="PK683_16425"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNH10084.1"
                     /translation="AGLTFITDAGAYFGGRWFGRHPAGLKISPKKTWEGYVTGLITSL
                     IFTLTVNYFWPQITGKPTPMGYLELVLFTPVMSIISVAGDLSESAMKRDAKMKDSASV
                     IPGHGGLLDLADAVFFTVPASYIYISIKEALGMAV"
BASE COUNT          390 a          350 c          309 g          455 t
ORIGIN      
        1 tcttatgcgg aacgcctgcc gaaattcagg cagcccccaa tccgattatt cagcagttta
       61 tcaatggaag gaccaagggg ccgatgattc tggatcattc ggagcagaac aaaaagaaat
      121 aattacttgt ccattttatc gacttctttt tccagctgag aaagctcctt gtccagctgg
      181 tcaatcattg catcgaaaat ctgcagtttc tcttcaatga cctgagcatt tttcttcagt
      241 tccttgttct ttttttcgtc actctttcca gcatttcctg gttcatcggt gttcgttttt
      301 ttcttgtaag agctgttctt gtggaaggaa taataaagcg ggtatgccgc tgccggaatt
      361 aaaatcagta aaagtattgt gatcaggttc atgagtgact gctcctaaat tggttcattt
      421 tgctgtttat ccaaatttca aaattaccgc cagccatgct gccgatcagt ttcataatgt
      481 cggaataacg gatccctaaa aatttatgtt tgtatgaatc ataatgcata tagtattcga
      541 aaggatcctt gaatgctcca tggtcttcta ccagctgttc ttcgcaggag aactgcgaga
      601 agcatggatc tttcccgttc caatcgcagg aaccttctga atgaatcccg aatccgcatt
      661 tctcgccgat cccttcgagg acttttcttg cttccagaaa gagttttctt tccactgaat
      721 agctgtcagt aggaatgtat gtgccgtcta cattgatttc cagatagcag tgagtgactg
      781 tttccggcat cagcgaatag acaacatccg gaaaacagtg tctgtaaaaa tcttttttta
      841 cagtgacgaa atgcatttga ctgttgatgt ctgcttcact gagaagtttc atcagcagca
      901 gtcctttcgt gcttgaagtt ccgtaaccga gttccattgt ctcttcagcc cctgcaaaat
      961 cgagtttatg agtaagtcca aaacgtattc tgtctctcac aaaatcatgg agtctgactg
     1021 ctttttccgg gggggagtct gcctctcctg caatattcag agcaatgttc ttcagctcgt
     1081 acatcctgct taaactgcca ttcctaatgc ttctttaatg ctgatatata tataacttgc
     1141 cggtaccgta aaaaacactg catcggccag atccagaagc cccccatgac ccggaatgac
     1201 tgaagcggaa tccttcattt tggcatctct tttcattgca gattcggaaa gatcacctgc
     1261 aacgctgatg atggacataa ccggagtaaa cagaacaagc tccagataac ccattggtgt
     1321 cggcttgccg gtaatctgag gccagaaata attgacagtc agtgtgaaaa tcagtgaagt
     1381 aatgagacct gtaacatagc cctcccatgt tttcttggga gagatcttca gaccggctgg
     1441 atgtcttcca aaccatctgc ctccgaaata ggcccctgca tctgtgatga atgtcagtcc
     1501 agcc
//
LOCUS       DAOQGJ010001205         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64339_length_1503_cov_2.189227, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001205 DAOQGJ010000000
VERSION     DAOQGJ010001205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64339_length_1503_cov_2.189227"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PK683_16430"
     CDS             <1..466
                     /locus_tag="PK683_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNH10085.1"
                     /translation="HTSFVSVHVEPELDDSINVVIEDKDLKVDTYRSSGAGGQHVNKT
                     DSAVRMTHIPTGIVVQCQNERSQIKNRATAMKMLKAKLYDLEKKKNEEEQAKKSGEKK
                     DIAWGSQIRSYVFHPYNLIKDHRTEHETANISPVMDGDLEPFILAYLKTQML"
     gene            complement(467..1204)
                     /locus_tag="PK683_16435"
     CDS             complement(467..1204)
                     /locus_tag="PK683_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10086.1"
                     /translation="MLSIKTFDKAAVLIMMMNVSLNAGDSFQYFGTASDLNKGSKLYT
                     DHHTETVQSGNHISSYIEYRDSSGKTFASKKISFGKSSQLPDFTLEDKRDGYLEGAEL
                     NGDKIRIFTRKNQNSPMQERTVTLPGDSVVDGGFDHYIRKHWDSFLAGKSRSFYMFAP
                     AKQDFYKFKAFKVNDKAFKGKDAFLVRVELDVFALGKILPAIQITYDKKTKRILKYEG
                     ISNINDSSGKSHFVRLEYDYPNLTASE"
BASE COUNT          441 a          315 c          298 g          449 t
ORIGIN      
        1 acatacctca ttcgtatccg ttcatgtgga gccggaactt gacgacagca tcaatgtggt
       61 aattgaagac aaggatctta aggtagatac gtacagatcc tccggtgcgg gaggtcagca
      121 cgtaaataag actgactccg cagttcgaat gacacatatt cctacgggaa ttgtggttca
      181 gtgtcagaac gaaagatctc agatcaaaaa cagggcaaca gcaatgaaaa tgctcaaggc
      241 caaactgtat gatctggaaa aaaagaaaaa cgaagaagag caggcaaaaa agtcaggcga
      301 gaagaaagac attgcctggg gttcacagat cagaagttat gtatttcatc cttataattt
      361 aattaaagat cacaggactg aacatgaaac agcgaatatc agtcctgtaa tggacggcga
      421 tctcgaacca tttattctgg cctatctcaa gacacagatg ctctgatcat tcacttgccg
      481 taagattggg ataatcatac tccagtctta cgaaatggct tttccctgaa ctgtcattga
      541 tattggaaat cccttcgtac ttcagaattc tcttcgtttt tttatcataa gtaatctgta
      601 ttgcaggaag aattttaccc agtgcaaata catccagttc cactcttacc agaaatgcat
      661 cctttccttt gaatgcttta tcattcactt taaaagcctt gaatttatag aaatcctgtt
      721 ttgccggagc gaacatataa aaacttctgc tctttcctgc tagaaaagaa tcccagtgct
      781 ttctgatata gtgatcaaat cctccgtcca ctacagagtc tcctggaaga gtaacagtcc
      841 tttcctgcat cggtgagttt tggttttttc ttgtaaagat tctgatcttg tccccgttga
      901 gctcagcacc ttccagatat ccgtctcttt tatcttcaag cgtaaagtca ggaagctgag
      961 atgatttgcc gaaactgatc ttcttggatg cgaatgtttt gcctgaactg tccctgtact
     1021 caatatagga actgatatga tttcctgact gcaccgtttc tgtatgatga tctgtataaa
     1081 gttttgaccc tttgttcaga tcagacgcgg tgccgaaata ctggaaactg tctcctgcat
     1141 tcagtgaaac attcatcatc ataatcaata ctgccgcctt gtcaaaagtt ttaatagata
     1201 acattgtaca tccctctatc tagatagacg gctggatttc aaaaaatcca cctttttttt
     1261 gagattcttt ctcagaaata gaatggagga tgtactttga aatttgcggt attgcattat
     1321 ctgcggcagc tctgattcag actgccgcaa tttaccggtc ttttactcag ccgagcgagc
     1381 gaaggcaata gagaggattt aagatgaaag tgtaattgag tctgctgaat ttcctgatga
     1441 cagagctttc agaaacagtt ctacatcttc ttcagaacgg atgcagtacc attttccttc
     1501 agg
//
LOCUS       DAOQGJ010001206         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64389_length_1502_cov_2.812716, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001206 DAOQGJ010000000
VERSION     DAOQGJ010001206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64389_length_1502_cov_2.812716"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PK683_16440"
     CDS             complement(<1..682)
                     /locus_tag="PK683_16440"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNH10087.1"
                     /translation="MPYNPSSSSKKHLHRIIQDGKEKSLVPPEFKPLMQRILEPIGLI
                     SLFSSIPMAWYNGIYFSVPGILGYFIISIIVDLFIQDDHALVRIYGPLGRLRYIFEEA
                     FRDKYLQYFNETNSDGRPIPRIVRDYIYQKAHNIKPVSSFGTELDIHDPEITTHSRIL
                     HRNFPGKIISTSYSVSVGEKRKNIKPFIIKNTINISGMSYGALNYRAAESLSIGAKDV
                     AYVNTGEGG"
     gene            complement(692..1438)
                     /locus_tag="PK683_16445"
     CDS             complement(692..1438)
                     /locus_tag="PK683_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10088.1"
                     /translation="MIRLLFLFTILFFFFPLYSQTKQDLLTLTDRFLLFPTGVIEAEL
                     ITKKKYVQYEKRNLKIISKGQAKRILFSRTAESPENVYISDGRESVHFASLHPYHSRK
                     ISREEIIDKILFTDFSFFDLSGFLFEKEFKPDRIEDYEVGALKFQKLTCFPVAMPQIS
                     KISLIFKMPERDLYRIDYFSRSGILMKSVKFRMGRVLFRRNGNKKEEEILKSIEITNA
                     DTNLETVLEFKSVVSDSEESADFTLENIRP"
BASE COUNT          434 a          307 c          306 g          455 t
ORIGIN      
        1 agcccccttc cccggtgttc acataagcca catcctttgc gccaatgctc agagactctg
       61 ctgccctgta gttcagagcg ccgtagctca tcccggaaat atttattgta ttcttaatta
      121 taaaaggttt tatatttttt cttttttcac cgacagagac agaataggat gtgctgatga
      181 tttttccggg aaaatttctg tgaagaatcc gcgagtgtgt agtgatttca ggatcatgga
      241 tgtcgagttc tgttccgaaa ctgctcacag gcttgatatt gtgcgccttc tggtagatat
      301 agtcccttac gatccgggga atcggtcttc cgtctgaatt ggtctcattg aaatactgaa
      361 gatacttgtc cctgaaggct tcttcaaata tatacctgag tcttccgaga ggtccgtaaa
      421 tccgtacgag cgcgtgatcg tcctgaatga acagatctac aataatactg ataataaaat
      481 atcccagaat ccccggaaca gaaaaataaa ttccgttata ccaggccata ggaatggaag
      541 agaaaagaga gatcagccca atcggctcca gtattctctg catcagaggt ttgaactcag
      601 gcgggacgag ggatttttcc tttccgtcct ggataattct atggagatgt tttttggaag
      661 aggaggaagg attgtacggc atttgaaaac ctcagggtct aatattttct aaagtgaaat
      721 ctgcactttc ttcagaatct gaaaccaccg atttgaattc cagaacagtt tccagatttg
      781 tatctgcatt tgtgatctca atacttttca gaatttcctc ttcttttttg ttcccgttcc
      841 tgcggaacaa aaccctgccc attctgaact tcactgactt cattaaaatc ccggatctgc
      901 tgaaataatc aatcctgtaa agatccctct ccggcatttt aaaaatcaga ctgatctttg
      961 aaatctgagg catcgcaacc ggaaaacagg tcagtttctg aaacttgagt gcaccgactt
     1021 catagtcttc aatcctgtca ggtttaaact ctttttcaaa cagaaaaccg gaaagatcga
     1081 aaaaactgaa atccgtaaag agaattttgt caataatttc ttccctggaa attttgcggg
     1141 aatgataagg atgcaggctg gcaaaatgga cggattccct gccgtcagaa atatacacat
     1201 tctcaggact ttctgcagtt ctggaaaaaa gtattctttt ggcctgacct ttggaaatga
     1261 tcttcaggtt tcttttttca tactgaacat attttttttt tgttatcagc tctgcttcaa
     1321 tgactcctgt cgggaaaagg agaaatctgt ctgttaaggt gagaagatcc tgctttgtct
     1381 gtgaatacag aggaaaaaag aaaaatagga ttgtaaaaag gaagagaagc cggatcatcc
     1441 tgcaggaaaa ttccagaaag tccggcattt tgcgaaaata attactttat gacttcattg
     1501 at
//
LOCUS       DAOQGJ010001207         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64450_length_1501_cov_3.089212, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001207 DAOQGJ010000000
VERSION     DAOQGJ010001207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64450_length_1501_cov_3.089212"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PK683_16450"
     CDS             <1..339
                     /locus_tag="PK683_16450"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803498.1"
                     /note="catalyzes the formation of N-carbamoyl-L-aspartate
                     from (S)-dihydroorotate in pyrimidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNH10089.1"
                     /translation="GGVRPHHYCLPVLKREEHRQRLVRAAISGNAKFFAGTDSAPHAK
                     HTKENACGCAGIYTAFGAVELYAEVFDRADSLDKLEFFLSEAGARFYGLQLNAGHMRL
                     KKEKSPQHSL"
     gene            complement(386..>1501)
                     /locus_tag="PK683_16455"
     CDS             complement(386..>1501)
                     /locus_tag="PK683_16455"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HNH10090.1"
                     /translation="KPSKNYCTKRVISSVQFEEIKNNDKMPTHKPFKLSPEDFAECSR
                     IIRIRNIPVWKYKRVKILLLLHEGLTYKKIESETDTNSHTISQWKSRYLEEGMEGLKD
                     RPRPGQTKKITEEVEAKVLAAVQEKPPAGKTHWSNYDLGAKVGLHPRRVCEILNRHNI
                     KPHLHRYFMVSNDPEFEQKASEIIGLYISPPKNAAVLCVDEKTSIQALDRMQPNLPLS
                     PNRTERSTFEYRRNGVTSLFAAFNTRTGEVTGDCKDSHTQYDFIDFLDKLDKVYKNKE
                     IHIILDNFSAHKTAAVEEWKTEHPNWKFHFTPTYSSWLNQVECWFSIISRKCIRRGVF
                     DSTKDLISKIKAFIKDYNKKAKPFVWKYNDPSSRITV"
BASE COUNT          349 a          351 c          315 g          486 t
ORIGIN      
        1 gggggcgtcc ggccgcacca ctactgcctg ccggtactca agcgtgaaga acaccgccag
       61 cgcctggtcc gcgcggcgat ttcaggcaac gcaaagtttt tcgccggtac agacagcgcc
      121 ccccatgcaa agcatacgaa agagaatgcc tgtggctgcg ccggtatcta caccgccttt
      181 ggcgcagttg aactgtatgc agaagttttt gatcgggcag actcgctcga caagctggag
      241 ttctttcttt ctgaagcagg tgcccgtttc tacggcctgc agctcaacgc tggacatatg
      301 cgcctgaaga aagaaaaatc cccgcagcat tcgttgtaaa cagtgcagtt ccgtctgttc
      361 tgacatagtg cactaagcgg gtgattcata cagtgattct ggaagaggga tcattatatt
      421 tccatacaaa aggttttgct tttttattat aatctttaat gaaagctttt atcttggata
      481 tcaagtcttt ggtgctgtca aaaacaccgc gcctgataca ttttcttgaa ataatggaaa
      541 accagcattc cacctgattc agccaggagc tgtatgtcgg agtaaaatgg aatttccaat
      601 tagggtgttc tgtcttccat tcttccactg cagcagtctt gtgcgcactg aaattatcaa
      661 gtattatatg aatctctttg ttcttataca ctttatccag tttatccaga aaatcaatga
      721 aatcatattg cgtatgtgag tctttacagt cgcctgtcac ttcaccagtc ctcgtattga
      781 acgcagcaaa taatgaagtc actccgttcc tcctgtattc gaatgtggaa cgctccgttc
      841 tgttcggact cagcggcagg ttcggctgca ttctgtccaa tgcctgtatg gaagtctttt
      901 catccacgca taatactgct gcatttttcg gagggcttat atacagtccg attatttcac
      961 ttgccttctg ctcaaattca ggatcatttg ataccataaa atatctgtgc agatgaggtt
     1021 ttatattatg acggtttaaa atctcgcaga ctcgtctggg atgcagtccc actttggctc
     1081 ccaagtcata attgctccag tgcgttttcc ctgcaggagg tttttcctgc actgctgcca
     1141 gcacttttgc ttcaacttct tctgtaattt ttttagtctg cccgggacgg ggtctgtctt
     1201 tcagcccctc cataccttct tccaggtatc ttgatttcca ttgacttatt gtatgtgaat
     1261 tcgtgtctgt ttccgattca atcttcttgt aagtcaggcc ttcgtgaaga agcagcagta
     1321 tcttgactct tttatatttc catacaggaa tatttcttat tctgattatc ctgctgcatt
     1381 ctgcgaagtc ttccggtgac agtttaaaag gtttgtgagt cggcatttta tcattatttt
     1441 ttatttcctc aaattgtaca gaagaaatta cccgcttagt gcaatagttt ttgctcggtt
     1501 t
//
LOCUS       DAOQGJ010001208         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64458_length_1501_cov_2.845781, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001208 DAOQGJ010000000
VERSION     DAOQGJ010001208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64458_length_1501_cov_2.845781"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..>1501
                     /locus_tag="PK683_16460"
     CDS             191..>1501
                     /locus_tag="PK683_16460"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HNH10091.1"
                     /translation="MVKRYFNTSGPNIPAKHYTLFRSRLIHNGILKVRDERCFTIWAP
                     RQTGKSTLFRQMAVELEKEGYKVCHINFENYRDASLESFLNTFIIELNRYWGTDFVYS
                     ELTVLFQNIRQIADRKFVLIIDEVEGINSDYLNGFLHSVRNLYHSRESHSLKSVILVG
                     VSNITGIMQDNASPFNIADELNVPYFTDAETAELLGQHTQETGQTFAPEVLAKISEIT
                     ANQPGLVNGFAKQMVDRNPEKTLIAVSDYEEVEEWYLTEAIDKNVANILKIAEQNRRF
                     LERLLFNEEEIPFEIDRPVIKTLHVNGLIKKDTDRNISFWVPLYRKRLFKALYPYSNG
                     ESKRMSESMFADSYLTSEGDINFDKLMESYRNHIRLRSFRPFREKDESGVYISIPEAA
                     MLYSFETFISIFVQELEGKMYRESIVSLGNSDLVINVRGKEFYIE"
BASE COUNT          447 a          315 c          320 g          419 t
ORIGIN      
        1 tctggaattt cctgtcatcc cgtaggaaag aggtcttgca tccactgcga tcaggggttt
       61 gttttttttc atattctctt ttcagtaaaa gacccagtgc ctggagcatc aactgaaatt
      121 ttgccgcttt acgcatccga tgatattctc ctttaccaaa gaacgctttt tcgacatttt
      181 gaatacagtt atggtaaaac gatatttcaa tacttcgggt ccgaatattc cggctaaaca
      241 ctatactctt tttcgctcaa gacttattca taatggaatt ttaaaagtca gggatgaacg
      301 ctgctttaca atttgggcgc ccagacagac aggaaaaagc accctgttcc gtcagatggc
      361 agtcgaactc gagaaagaag gttacaaggt ctgccatatc aattttgaga attacaggga
      421 tgcatctctg gaatcctttc tgaatacttt cattattgaa ttaaaccggt attggggaac
      481 agatttcgta tacagtgaac tcaccgtact gtttcagaac atcaggcaga ttgcagaccg
      541 gaaatttgtt ctgatcattg atgaagtgga aggcatcaac tcagactatc tgaacggatt
      601 cctgcattct gtccgcaatc tctatcattc ccgtgaatct cattctctca agtccgtgat
      661 tttagtagga gtatccaaca ttacagggat tatgcaggac aatgcaagtc ccttcaatat
      721 cgcggatgaa ctgaatgtcc cgtatttcac cgatgcagaa actgcagaac ttctcggaca
      781 gcatactcag gagacaggac agaccttcgc gcctgaggta ctggcaaaga tcagcgagat
      841 cactgccaat cagcctggcc ttgtaaatgg atttgcaaaa cagatggttg acaggaatcc
      901 ggaaaaaact ctgatcgctg tttcagatta tgaagaagtc gaagagtggt acctgacaga
      961 agcaatcgac aagaatgtag ccaatattct gaagattgcc gaacagaaca gacgttttct
     1021 ggaaaggctt cttttcaatg aagaagaaat cccttttgaa attgacagac cggtgatcaa
     1081 aactctgcac gtaaacggac tcataaaaaa agataccgac aggaatattt cgttctgggt
     1141 gcctctctac cggaaacgtc ttttcaaggc tctttatcca tacagcaacg gtgagtccaa
     1201 aagaatgtcc gagtccatgt tcgctgattc ctatctgact tctgaagggg atataaattt
     1261 tgacaagcta atggaaagtt acagaaatca tatccgtctc agaagtttcc gtcctttccg
     1321 ggaaaaggat gaatcaggcg tttacatatc cattcctgaa gcagcaatgc tttacagttt
     1381 tgaaacattc atcagcatat ttgttcagga actggaaggg aagatgtacc gtgaatcgat
     1441 tgtgtctctc ggaaattccg atctggtcat caatgtgcgg ggaaaagaat tttacataga
     1501 g
//
LOCUS       DAOQGJ010001209         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64510_length_1500_cov_3.737716, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001209 DAOQGJ010000000
VERSION     DAOQGJ010001209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64510_length_1500_cov_3.737716"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..448)
                     /locus_tag="PK683_16465"
     CDS             complement(17..448)
                     /locus_tag="PK683_16465"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HNH10092.1"
                     /translation="MKSPIWILLNILCFFSVFVYAQDKTKPDENEVNKSPKVRFVNKS
                     NDRATDYTKDKDYQLGKSLAEKISKGKNYAEAGGVRIERYKADSEDRHGADIITVKKN
                     FHVGHIHSISRILSGYVQSSFDYPKKEADTLVFYILFLLFF"
     gene            complement(1117..>1500)
                     /locus_tag="PK683_16470"
     CDS             complement(1117..>1500)
                     /locus_tag="PK683_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10093.1"
                     /translation="MFFPLLMIPALILVMTYIYFQADRKHPSETGKTPRLSFRCAGII
                     GWISYKGPFIRVSLYEDFIIISYRKIIMLKFSEISVKKEKFLFNKTLRIEHGNSEYPD
                     KIKLFVSNYAVFLDFLRTKSVKISE"
BASE COUNT          452 a          314 c          293 g          441 t
ORIGIN      
        1 taaattcaat caaacactag aaaaataata aaaaaagaat ataaaatacc agagtatctg
       61 cttctttttt aggatagtca aaagacgact ggacataacc ggatagtatt ctggaaatgg
      121 aatggatatg gcctacatga aaattctttt tcactgtgat tatgtctgca ccgtgcctgt
      181 cttctgagtc ggccttgtat ctttcgatgc ggaccccgcc ggcctccgca tagtttttgc
      241 ccttgctgat tttttcggcc agagacttgc ccagctgata atccttgtcc ttagtataat
      301 cagtcgctct gtcatttgac ttattgacaa atctgacttt aggagacttg ttcacctcat
      361 tttcatcagg ttttgtctta tcctgtgcat acacaaaaac ggagaaaaaa cacaaaatat
      421 tcaagagaat ccaaattgga cttttcatca gtacccccag aaacacacta ttgaactact
      481 gcaaaaacct caaggttttt gcaagactgc ggccgttttc gaaaaaaaca gcttagatcg
      541 gagatgagca aacttgcagt tcacagactc aagtctcaat ttcagtcaaa ttcatcagcc
      601 tatctttatc agtatcggcc aaaataagga ctgatgcgta aatttcatgg attttagact
      661 gagacggaac atatagacga aggatttctt tttcaaaaac tttccttctc cggttttcga
      721 accggaaaat aaaaaaactg tccggatctt tttttgggag gctgtccaaa ctgcattgta
      781 cgttatgact cactttacgc tttcccggct gcctgtgcct ttttggaagg aaagcctgac
      841 tgccaagttg tctgggctgg gttcacagtg agccgtgtcg aactatgacc ctggcagcaa
      901 tccttcaccc tttcgaacct gctcagggca aacagctgaa ggaatgacaa atcattgctg
      961 attgcacagc attaggctgc cggctgtttg aggactcgca aatagaaaga cgggcgccga
     1021 aactcagaaa ccggctaaac ttatgcaggc tttttcctat gacggttcgt aaaaattgca
     1081 gattctatgc actgtattct ttttgcgcaa ggtgagttac tcggaaatct tcaccgattt
     1141 tgttcgaagg aaatccagaa aaacagcata gttggaaaca aaaagcttaa tcttgtcagg
     1201 atattccgaa tttccatgtt caatgcgaag agttttattg aaaaggaatt tttctttttt
     1261 gacgctgatc tcactgaatt tcagcattat gattttgcga taagaaataa tgataaaatc
     1321 ctcataaaga gaaaccctta taaaaggtcc tttgtaggaa atccatccga taattcctgc
     1381 acatctgaaa ctcagtctgg gcgtctttcc tgtttctgaa ggatgttttc tgtctgcctg
     1441 aaaataaata tatgtcataa ccaggatcag ggcaggaatc atcagcaaag gaaaaaacat
//
LOCUS       DAOQGJ010001210         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64519_length_1500_cov_3.268512, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001210 DAOQGJ010000000
VERSION     DAOQGJ010001210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64519_length_1500_cov_3.268512"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="PK683_16475"
     CDS             complement(<1..1490)
                     /locus_tag="PK683_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH10094.1"
                     /translation="MRKKQGAVLFTAGLLIFFVSVVNDILKTSGYLSTPLMASYGFLS
                     FVFFQAVLLSSRFAKAFVDAEQLTEELRLLSAELERKVEVRTLDLQNAKSETDTLNEI
                     AVKVNSNLDIGSIINVILKHIQEKYDINICFVFMLDSVRERISVFSSSVPAAAGRRMI
                     RLVSNLSINLNEDLDIHKYVLDSRKALYLKEVRKTGARIENFFKIRLKIKSFICIPLV
                     LKDEVIGFLDFSRMGRQISVSEADFAYLQILANQAAGAIRNASLMKEREEAVRSAEIE
                     KGIALIAQREAEIERMKSERLILNILPEKTAQELKEKGYTDPVFIESAAVMFTDFQGF
                     TEISELLSPQDLIQGLDFYFSAFDQIIQKHNLEKLKTVGDSYMCAGGVPRINAAHAVD
                     SVLAALEIQSFMNENSADRHNGSVKWKLRIGIHSGPVIAGVVGEKKFAYDVWGDTVNT
                     ASRMESSGEENRINISGAVYGKVKDFFDCRYRGRISAKNKGEIDMY"
BASE COUNT          415 a          368 c          269 g          448 t
ORIGIN      
        1 aagtacatat cgatttcacc cttgttttta gcggatattc ttcccctgta tctgcagtca
       61 aaaaaatctt ttacttttcc gtaaactgca ccgctgatat taattctgtt ttcttctccg
      121 cttgactcca ttctcgatgc tgtatttacc gtgtcccccc agacatcgta ggcaaatttc
      181 ttctcaccga ccactccagc tatgaccggt cctgaatgaa tgccgatccg aagtttccat
      241 ttgacagatc cgttgtgcct gtctgcagaa ttttcattca taaaagactg gatctccaaa
      301 gctgccagaa ctgaatcgac agcatgagcc gcattgattc ggggaacacc gccggcacac
      361 atataactgt ctccgactgt tttaagcttt tcaagattat gcttctgtat gatttgatcg
      421 aatgcagaga aataaaagtc gagcccctga atgagatcct gcggtgaaag cagttcagaa
      481 atctccgtaa aaccttgaaa gtccgtaaac ataactgctg cggattcgat aaagacagga
      541 tctgtatatc ctttctcctt cagttcctgc gcagtctttt ccggcagaat gttcaatatc
      601 agtctttccg atttcattct ttctatttca gcttctctct gcgcaatcag tgcaatgccc
      661 ttttcaatct ctgcgctcct gacagcctcc tctctctcct tcatcaggct ggcgttcctg
      721 attgcaccag ctgcctgatt agccaatatc tgaagatagg caaaatctgc ctcactgact
      781 gatatctgcc tgcccattct gctgaaatcg agaaagccga tgacttcatc tttcaataca
      841 agaggaatgc atataaacga tttaatcttt agacgaatct taaaaaaatt ttcaattctc
      901 gctccggttt ttctgacttc tttcagatac agagctttcc tggaatccag cacatactta
      961 tgaatatcca gatcttcatt caggtttatg ctgagatttg atacaagtct gatcatccgt
     1021 cttcctgcag cagcaggaac cgatgaactg aatacggaaa ttctctctct gactgaatcc
     1081 agcataaaaa caaaacatat attgatatca tatttttcct gtatatgctt aagaatgaca
     1141 ttaattattg agcctatatc cagattcgaa ttgaccttta ctgcgatttc attcagtgta
     1201 tcggtttctg atttcgcatt ctgaagatcc agcgttctga cctcaacctt tctttccagt
     1261 tcagcagaaa gaaggcgaag ctcttcggta agctgttccg catccacaaa agcctttgca
     1321 aatcttgaag aaagcagtac ggcctgaaaa aagacaaatg acagaaaacc gtaacttgcc
     1381 ataagaggag tggacagata acccgaagtc ttcagtatat cgtttacgac cgaaacgaaa
     1441 aaaatgagaa ggcccgcagt gaagagaact gcaccctgtt tcttccgcat cagactctgc
//
LOCUS       DAOQGJ010001211         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64614_length_1499_cov_2.651662, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001211 DAOQGJ010000000
VERSION     DAOQGJ010001211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64614_length_1499_cov_2.651662"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="dxs"
                     /locus_tag="PK683_16480"
     CDS             complement(<1..1198)
                     /gene="dxs"
                     /locus_tag="PK683_16480"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNH10095.1"
                     /translation="MSNYPLLEKIENPTDLRKLPLEKLPGVCREIREYIIDTLSEIGG
                     HFASNLGAVELTVAIHYAFETPKDRLIWDVGHQTYPHKILTGRREKLKTVRKFEGISG
                     FPKREESEYDLYNTGHAGTSISQLLGEAVGRDITKQKYHCLAVIGDASIGCGMALEAL
                     NHGGHIKPNCLVILNDNFMSISKNIGSISSYLNNLITSHIYNKYKKYYYSLLKWFPLI
                     GPLLLSLSRKFERTFKNFLVPGGLFEDLGFRYFGPVDGHDVVGLVKVLDKIKDEEGPL
                     LLHVITQKGKGYAHAEDDPIKYHGVTPFEKESGKMNSQETKVGFSKIVGECLIRLTEK
                     NPKVAAITPAMIEGSGLKAYSQKFPDNLFDVGIAEQHSVTFASGLLGGGVIPYMCIYS
                     TFMTRAL"
     gene            complement(1191..>1499)
                     /locus_tag="PK683_16485"
     CDS             complement(1191..>1499)
                     /locus_tag="PK683_16485"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH10096.1"
                     /translation="GPAVVGNMGSSHRMDYTCMGDTVNLGSRLEGSNKMYATRIIISE
                     YTYDRVKDRVYARELDLVKVKGKNQPVRIYELIGLVDDNDAEKLVRQEIVRKKENTNE
                     "
BASE COUNT          390 a          355 c          282 g          472 t
ORIGIN      
        1 ccagcgccct tgtcataaac gtagaatata tgcacatata tggaatgact cccccgccga
       61 gaagtcctga tgcaaaggta actgagtgct gttctgcgat tcccacatcg aaaagattgt
      121 ccggaaattt ctggctgtat gctttcagtc cgcttccctc aatcattgca ggagtaattg
      181 cagcaacttt cggatttttt tcagtgagac gtataagaca ctctcctacg attttgctga
      241 atcctacctt ggtttcctgc gaattcattt taccgctttc tttttcaaat ggagttacac
      301 cgtgatactt gatcggatca tcttctgcat gagcataacc cttgcctttc tgagtgatca
      361 catgaagcag aagaggacct tcctcatcct taatcttgtc cagaactttg acaagaccca
      421 ctacatcatg accgtccaca ggaccgaaat accggaatcc cagatcttca aagagccccc
      481 cgggaacaag aaagttcttg aatgttcttt caaacttcct ggaaagactg agaagaagag
      541 gtccaatgag gggaaaccat tttaataaag agtaataata ttttttatac ttattatata
      601 tatgtgatgt gatcaggttg ttcagataac tggaaatgga accgatattc ttggaaatcg
      661 acataaagtt gtcgttcagg atcacaagac agttaggctt gatatgacct ccgtgattga
      721 gtgcttccag tgccattccg catccaatgg aggcatctcc aatcactgca aggcagtggt
      781 atttctgttt tgtaatgtcc ctgcccacag cctcgccgag gagctgagaa atcgaagtgc
      841 cggcatgacc ggtattgtaa aggtcatact ccgattcttc tcttttagga aatccggaaa
      901 tcccttcaaa tttccttaca gtcttaagtt tttctcttct gcctgtgaga attttatgag
      961 gataggtctg atgtcccaca tcccagatca gtctgtcttt cggtgtttca aatgcataat
     1021 gaattgctac agtcagttca acagcaccca ggttgctcgc aaaatgcccg ccgatttcag
     1081 acaatgtatc aataatgtat tctctgatct ctctgcatac ccccggaagt ttttccagag
     1141 gcagctttct caaatcagtc ggattttcaa ttttttccag taatggatag ttactcattc
     1201 gtattttctt tttttctcac tatctcctga cgtactaact tttctgcgtc attgtcatct
     1261 acaagtccga tcagttcata aattcgtaca ggctgatttt ttccttttac tttcacaaga
     1321 tccagttctc ttgcatatac tctgtccttg acccggtcat aagtgtattc agaaataatg
     1381 atccttgttg catacatttt attggaacct tcgagtctcg agccgagatt cactgtgtct
     1441 cccatacagg tatagtccat cctgtgagaa cttcccatat ttcccacaac tgccggacc
//
LOCUS       DAOQGJ010001212         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64622_length_1499_cov_2.464681, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001212 DAOQGJ010000000
VERSION     DAOQGJ010001212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64622_length_1499_cov_2.464681"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="PK683_16490"
     CDS             complement(<1..1383)
                     /locus_tag="PK683_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH10097.1"
                     /translation="MAGLKVYIIEDDRITLSQISYFLSKRGIVISGHSETGENALKEI
                     PLIKPDVILCDIYLKGDLDGIQTSERLAEIYPSPVLFMTANDDQALLERSIHARPYGF
                     LKKPLRFDALVNTIQLSFEKFLTENLLKEKEKYFQTILSGISDLSLFTLNKELKISSW
                     NDTAVRMYEYSASETIGNPFSMLFYRQSDLTLSAEDIFNEFQDRKLHRKKDGSEFYAE
                     FRITALNSIDSPDDDYSVLIQNVHSRVILEQEKEEHRIRLEKYSQELEERVRERTAEL
                     KGLNDSLKNEIELNRSFQNIILKNQKNLLEAQKLAHIGDWEIYFGAEELKLSPEVFSV
                     LEISENSVTQNEFLEIIYGRNSEGDILEHLNHRLLNENEISYEREYQISGRIKYLQIK
                     IKPIFEEKRLTGILGTLHDETERILHSKEINKALEKEKELNRLKNRFVSMISHEFRTP
                     LTVILTNVQLM"
BASE COUNT          390 a          299 c          298 g          512 t
ORIGIN      
        1 catcagctga acatttgtga gaatgactgt cagaggagtt ctgaattcat gagaaatcat
       61 ggaaacaaac cggttcttca gtctgttcag ttccttttct ttttccaaag ccttgttgat
      121 ttccttggag tgcagaattc tttctgtttc atcatgaagg gtgcctaaaa tgccagtcag
      181 tcttttttcc tcaaatatgg gttttatttt aatctgaagg tattttattc tgccggatat
      241 ctggtactct ctttcatagc tgatttcatt ttcgttgagg agtctgtgat tcagatgttc
      301 gagtatgtcc ccttcagaat tccttccgta aatgatttcc aggaattcat tctgagtgac
      361 tgaattttcc gatatttcga gaacggaaaa cacctcagga gaaagtttca gttcctcggc
      421 accgaaataa atttcccagt ccccgatatg cgcgagcttc tgagcttcca gaagattttt
      481 ctgatttttc agaataatgt tctgaaagga tctgttcagt tcaatttcat ttttcagact
      541 gtcgttcagc cctttcagtt cagcagttct ttctctgact ctttcctcaa gttcctgact
      601 gtatttttcc agacggatcc tgtgttcttc tttttcctgt tccagaatga ctctggaatg
      661 aacattctgg atcagcacag agtaatcatc gtccgggctg tcaatgctgt tgagagctgt
      721 aattctgaat tccgcataga attctgatcc gtcctttttc ctgtgcagtt ttctgtcctg
      781 aaattcattg aatatgtcct ctgcggaaag agtcaggtcc gactgacggt aaaacagcat
      841 tgaaaatgga tttcctattg tttcagaagc agaatactcg tacattctga cagcagtgtc
      901 attccaggaa gatatcttca gttctttgtt cagagtgaac agtgaaagat cgctgattcc
      961 gctcagaatt gtctgaaagt atttttcttt ttccttcagc agattttctg tgagaaactt
     1021 ttcgaaactc agctgaattg tatttacaag tgcatcgaat cggagaggtt ttttgaggaa
     1081 tccgtacggc ctggcatgaa tggatctttc cagaagagcc tgatcatcat ttgcagtcat
     1141 aaaaagcacc ggcgagggat agatttctgc gagtctttct gaagtctgaa tcccgtcaag
     1201 atcacctttg agatagatat cgcaaaggat gacgtcgggt tttatcagag gaatttcctt
     1261 gagcgcgttt tcacctgttt cagaatggcc tgaaatgact attcctctct ttgataaaaa
     1321 ataggaaatc tgtgagagtg taattctgtc atcttcaatg atataaactt tcagtcctgc
     1381 catctacagc attaaaattt taccggattc tcttttacaa caaaaaaaat cttgtgcgca
     1441 gaggcctgta ttctttataa tttttgtaca accatggcag aaaaaaaagg atttgaaat
//
LOCUS       DAOQGJ010001213         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64673_length_1498_cov_3.146916, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001213 DAOQGJ010000000
VERSION     DAOQGJ010001213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64673_length_1498_cov_3.146916"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PK683_16495"
     CDS             complement(<1..334)
                     /locus_tag="PK683_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002179820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNH10098.1"
                     /translation="MRKLEESIIAEVIIDTGPIVAFFDESDRYCIPFRNFLKGFRGRL
                     CTTLAVVTEASYLLDECKPVQLDLMEWIKDGAIEIRDIGNSDFSLIHKYMTKYQDTPM
                     DFADASLVI"
     gene            complement(303..551)
                     /locus_tag="PK683_16500"
     CDS             complement(303..551)
                     /locus_tag="PK683_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNH10099.1"
                     /translation="MISLRLPKDMEEKLTEISVAKNKTKTYIIKESLEMYLRSQEPVL
                     SAFELGKKYFGKYSSGRTDNSVNFKDRIVEKIRRKHNR"
     gene            1109..>1498
                     /locus_tag="PK683_16505"
     CDS             1109..>1498
                     /locus_tag="PK683_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH10100.1"
                     /translation="MKPEKKEARMELRGETEVVLKRYFAAPRELVFDCHTKPELMRRW
                     LIGPPGMVLETCENDLKTGGRYLYVYAGADGNKVGVYGKFLEVNIPEKFANTENYAMD
                     MSVFNPNAPEDPNATVESRTFTAEGKGT"
BASE COUNT          449 a          323 c          312 g          414 t
ORIGIN      
        1 gtatcacaag acttgcatcc gcaaagtcca tcggtgtgtc ctgatatttt gtcatatact
       61 tatgaatcag agagaaatca gaattgccga tatctcgaat ttcaattgcg ccatccttga
      121 tccattccat cagatccagc tgaacaggct tgcattcatc cagaagataa gatgcttcag
      181 tcacaacagc cagggtagta cagagtcttc cgcgaaatcc tttcagaaaa ttcctgaacg
      241 gaatgcagta ccggtcgctt tcgtcaaaaa aagcgacaat cggacctgta tcaatgatca
      301 cttcagcgat tatgctttct tctaattttc tcaacgattc tatctttgaa attgactgaa
      361 ttgtctgtgc gtccagaaga atactttccg aaatatttct tccccagctc aaacgcagag
      421 agcacaggct cctgggaccg aagatacatt tccaacgatt ctttgatgat gtatgtcttc
      481 gtcttgtttt tggcgactga aatttctgtc agtttttctt ccatatcttt cggcagtctg
      541 agacttatca ttctctgcaa tccttgtatt acaaggtatt acaatatatc aaaatagaca
      601 agaatttttt atttttttct tacagggctt ctcctgtaaa cctgcaccgc acttctgttt
      661 tccggcagtc tcaaatccat atttagatac acagcacccg gacggataac cagattcgaa
      721 aataatatct tgcacaggct gagcgaatct cgacttccct ttctttttaa agctgacaat
      781 aagccgccga aggacgatga aaaaaacttg acatcagaat cttatataac ttatgtgtta
      841 tataagattc tgattatgaa gaatatgaat tccgcctcgc gaaaactgaa tcgacggtaa
      901 tgtagctttc taaaccctgc gatgagccgt tttgccgtct cacgatattt taaatttttg
      961 gagaatgctg aacttgtctc aaatgcaatc cgtgccgaaa ttagactgta ccgctcactt
     1021 gagaaatggt gccggatgca ggagaaactt tgcagatgct tgggactggc ggaagaaata
     1081 aacaatgcag acagaaggag ataaaaaaat gaaaccagaa aaaaaagaag caaggatgga
     1141 actccgtgga gaaactgaag tcgtgctcaa acgttacttc gcagcaccgc gtgaattggt
     1201 tttcgactgc cacacaaaac ctgagctgat gcgccgatgg ctgatcggtc ccccgggaat
     1261 ggtgcttgaa acctgcgaaa acgatcttaa gaccggcggc agatacctgt atgtatatgc
     1321 aggtgcagat gggaacaagg tcggtgtata tgggaaattt ctggaagtga atattccgga
     1381 gaaattcgcg aatacagaga attatgcaat ggatatgtct gtcttcaatc cgaacgctcc
     1441 cgaagatccg aatgctacag tcgagtcacg cacttttaca gcagaaggta aaggaact
//
LOCUS       DAOQGJ010001214         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64676_length_1498_cov_2.990991, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001214 DAOQGJ010000000
VERSION     DAOQGJ010001214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64676_length_1498_cov_2.990991"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK683_16510"
     CDS             complement(<1..284)
                     /locus_tag="PK683_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNH10101.1"
                     /translation="MHPFVVFRDDRKILKEKIDSMIQSLKSKINSILLKAFTFLFFTS
                     CVSIPQGIQPISNFQLEKYLGKWHEIARLDHSFERGLSNVTAEYSLKEDGS"
     gene            578..1309
                     /locus_tag="PK683_16515"
     CDS             578..1309
                     /locus_tag="PK683_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenesis-related family 1 protein"
                     /protein_id="HNH10102.1"
                     /translation="MKKIILLITVLFSVSSVLSYSKAEMDKVYNYLKTKWSYRTAKGE
                     KITVESLSWRSSGNDYEVIANGKSLVLEEVFDSSGINLLNPSLKADVSAVEPKGKEPG
                     NLKGFTEAHNKYRRETGVPDLEWDTKLAAYAQEWADHLKSANGCNMKHRPRSGKYTQK
                     HGENLAWASGKEMEPSEVVDDWYSEIKDYNYSKNSCSGVCGHYTQVVWKNSRKLGCAM
                     AKCQDSEIWVCNYDPPGNYNGEKPY"
BASE COUNT          480 a          269 c          324 g          425 t
ORIGIN      
        1 gaaccgtctt ccttcagtga gtattctgct gttacattgg aaaggcctct ttcaaaagag
       61 tgatcaagtc ttgcgatttc atgccatttt cccagatatt tttccaactg aaaattactg
      121 ataggctgaa ttccctgcgg gatgctgacg caagaggtga aaaacagaaa cgtaaaagct
      181 ttcagaagga tggaatttat ctttgatttg agtgattgaa tcatggagtc gattttttcc
      241 ttcaggattt ttctgtcatc tctgaacacg acgaatggat gcattctctc tatcagcaaa
      301 atgggccatt ctgcccgcaa aaagaatata ttattccccg aatttccaat agccgttggg
      361 tcgagcatag gaagaggctg tcaaacccgg cggcttctgc agagcagtga agtttttttc
      421 cggaaaaacc ttcatcatcc gatgcagcat atgcaaagac tttcgaagat cagtatatcg
      481 taaaaaagga aatagatttc ctttttcaag agaaatactt gttgagattt tccgataaaa
      541 tctttgactt tttaattatc cagaaaataa tttacatatg aagaagataa tattattgat
      601 tacagtattg ttcagcgtaa gttctgttct ttcttattcc aaagcagaga tggacaaagt
      661 ttacaattac ctgaaaacta aatggtccta ccgcactgca aagggagaaa aaataacagt
      721 agaatcactc agctggagga gttcaggcaa tgattatgaa gtcattgcaa atggaaagtc
      781 tcttgttctg gaagaagtgt ttgacagcag tggaattaat ctgctgaatc cttcattaaa
      841 ggcggatgtg agtgctgttg aacctaaagg aaaagaacca ggcaatctga aaggatttac
      901 tgaagcccat aataaatacc gcagagaaac cggggtgcct gatttagagt gggacaccaa
      961 actcgcagcg tatgcacagg agtgggcaga tcatttaaag tcagcgaacg gctgcaatat
     1021 gaagcacaga cccagatcag gcaaatacac tcagaaacac ggagaaaacc ttgcatgggc
     1081 atcgggaaag gaaatggaac cttccgaagt agtagacgat tggtacagtg aaataaaaga
     1141 ctataactat tctaaaaatt cctgttccgg tgtgtgcggc cactacacgc aggttgtatg
     1201 gaaaaactca agaaaactgg gctgtgcgat ggcaaagtgt caggactctg aaatatgggt
     1261 ctgcaactat gatcctcctg gaaactataa cggagaaaaa ccttattgac tttcgggcag
     1321 tgttccggta aaaaattgca ggatttctat gagattctgc agatgtaaaa ttatattata
     1381 actataaaat atttattttt aaattaatag tgttcatctc ctctctctgt gttttttgac
     1441 atttgtcaaa gactttttgg gcagttcaga aatttttccg ttgactttcg gaacggga
//
LOCUS       DAOQGJ010001215         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64710_length_1498_cov_1.791407, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001215 DAOQGJ010000000
VERSION     DAOQGJ010001215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64710_length_1498_cov_1.791407"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK683_16520"
     CDS             complement(<1..444)
                     /locus_tag="PK683_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10103.1"
                     /translation="MKKYKKLFYILSVFFAVFLYAEESGLPAKKGNQKKIPVKNSPPV
                     RPETSNNPESPEKKGNVNSEKEDWEKDIDALETKKNENGPAEDDDWEKDLEKDLKEQE
                     NSRKGFQDSRGTASPIQSQNQMNRSAQNLMMDIAASVDIVGVYDKN"
     gene            complement(512..1261)
                     /locus_tag="PK683_16525"
     CDS             complement(512..1261)
                     /locus_tag="PK683_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10104.1"
                     /translation="MRYHIVILTFILGMISDCEVFKAKKKSDNSSALAAAFLASQGGC
                     TSSTASSIGGSTRTSFNVSGTSCASGLAAIGFTGEGLTAGLTGSGTGSRLASTGTFSA
                     GGEKKMNIEVTFLLTDSSGYLDVIGNASVSGTQATGPAIRLKSSGTDVLASGSSTGSA
                     PDSGGNTAAGTGSEKTYCLEFHEENGTHLFGWPKSCASLSDSEKGSYTINKEGLTSAN
                     PGSRMGFILNKAVLKSFIVTSGKIGTAGSLQ"
     gene            1395..>1498
                     /locus_tag="PK683_16530"
     CDS             1395..>1498
                     /locus_tag="PK683_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HNH10105.1"
                     /translation="MKDLTDKQQLIISFITDFIMEKGYPPTIREVALG"
BASE COUNT          329 a          359 c          303 g          507 t
ORIGIN      
        1 gttcttgtca tacacaccta ctatgtctac ggatgcggca atgtccatca tcagattttg
       61 agcagatctg ttcatctgat tctgggattg gatcggagaa gctgtccctc tagagtcctg
      121 aaatcctttc ctgctgtttt cctgttcttt cagatctttt tccagatcct tttcccaatc
      181 atcgtcctct gccggtccgt tttcattttt ttttgtttca agagcgtcta tgtctttttc
      241 ccagtcttcc ttttccgaat tgacgttccc ttttttttcg ggcgactccg gattatttga
      301 ggtttccggt ctgacaggag ggctgttttt gacagggatt ttcttctgat ttcccttttt
      361 tgcaggaagt ccgctttctt ccgcatacag aaatactgca aaaaatacag acaaaatata
      421 gaataatttt ttatattttt tcaatcaaac tccttaatga attttgagcc ataccctttt
      481 cgtcctggta tgcctcagca tcccagtctt tttactgaag gcttcctgca gtgcctatct
      541 ttcctgatgt gacaatgaaa cttttcagga cggccttgtt cagaataaat cccatcctgc
      601 ttccgggatt tgcgctcgtc agtccttcct tattgattgt ataacttcct ttctccgagt
      661 cggaaagaga ggcgcaggac tttggccatc cgaacagatg agttccgttt tcttcatgga
      721 actccagaca gtatgttttt tcagatcctg ttcctgcagc cgtatttccg ccggagtccg
      781 gtgcggatcc tgtcgatgaa cctgatgcaa gcacatctgt tcctgatgat ttcaatctga
      841 ttgcaggtcc tgtcgcctga gtgccggaga cggaagcatt tccgatcaca tccagatagc
      901 cggaagaatc agtcagcaga aaagtcactt cgatattcat tttcttttct ccgcctgcac
      961 tgaaagttcc tgtgcttgca agtctgctgc ctgtccctga tccggtgagt cctgccgtca
     1021 gcccctcgcc ggtaaatccg atggctgcaa gtcctgaggc acaggatgtc ccgctgacat
     1081 tgaacgaggt tcttgtgctc ccgccgatcg aggacgcggt cgagcttgtg cagcctccct
     1141 gcgatgcgag aaatgctgct gccaatgcgg aactgttgtc cgattttttc ttggctttga
     1201 aaacttcaca gtcggatatc attcctaaga tgaatgtaag tattacaata tggtatctca
     1261 atttcttctc ctttttctgt aatgcaatag agttgcattt gcagttcagt tgcattaacc
     1321 taaaaccctc tttttttttc aacttttttt ttcatgagtt gacataaaca aatattaagt
     1381 aattgttgtt tagtatgaag gacttaacag acaaacagca gctgatcatc agctttatca
     1441 ctgattttat aatggaaaaa ggttatcctc ccactatccg tgaagtggct ctaggttt
//
LOCUS       DAOQGJ010001216         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64723_length_1497_cov_5.471567, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001216 DAOQGJ010000000
VERSION     DAOQGJ010001216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64723_length_1497_cov_5.471567"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PK683_16535"
     CDS             <1..907
                     /locus_tag="PK683_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10106.1"
                     /translation="GTFGKMYTAVSAFSLGQPGWMNKIPYLSRIQTKHSGKVSVFSLV
                     YVPDKAAGKSEGIEKNFQDLPIGKVDHIVYTRYISEIYDNLGETVFILDKNRKILWKG
                     HTFDAEKILERLVSGELKEPDLLAYAGMCEDFEAIPTVRSYYSIKSAEFKKIKSEALK
                     ILKIMPDSHSILTHLVNLSLLAVRKKACTKQEAEKVCADINIENFSSGQLCRLLGYFH
                     SEYTELFPPKEFSLRLFSQAVKLEPNSADNLRQCIELLPLLKNIPEIRDVINEAVEQF
                     SKESSDSAEKRKLKDLLKKKSGSKK"
     gene            974..1327
                     /locus_tag="PK683_16540"
     CDS             974..1327
                     /locus_tag="PK683_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10107.1"
                     /translation="MKKKWMIFTAAALLASAGLSAENITLQIKKEELREENGKILFEP
                     DRRVMRAKYDTYVFDKKNFESVAGKKDKMPGSFKLNIESKPGTVKSVNPKEGAAPQGG
                     IKTRYFEATALKISK"
BASE COUNT          506 a          284 c          331 g          376 t
ORIGIN      
        1 gggtaccttc ggaaaaatgt atacagcagt aagtgcattc agtctgggac agcccggatg
       61 gatgaacaaa atcccttacc tctcgcgcat ccagacgaag cattcaggaa aagtttctgt
      121 cttcagtcta gtttatgttc cggataaggc ggcaggcaaa tcagaaggaa tagaaaaaaa
      181 ttttcaagat ctgccgatcg gaaaagtcga tcacattgta tatacaaggt atatctctga
      241 aatatacgac aatctgggag agacagtatt cattctggac aaaaacagaa aaatactctg
      301 gaaaggtcat acattcgatg cagagaagat tctggagaga ctggtctccg gagaactgaa
      361 agaacccgat ctgctggctt atgcagggat gtgcgaggat tttgaagcta ttccgacagt
      421 aagatcttac tacagcatca aatccgcaga attcaaaaag ataaaatcgg aagcactgaa
      481 gatcctgaag ataatgccgg actcgcacag tattctcacc catctggtca atctgtctct
      541 tcttgccgtc agaaaaaaag cctgcacaaa acaggaagca gaaaaagtat gtgcagatat
      601 aaatattgaa aatttctctt caggccagct ctgcagactt ctgggttatt tccactccga
      661 atatacagaa cttttcccgc ccaaggaatt ctcactgaga cttttttcac aggcagtaaa
      721 actggaaccg aattctgctg ataatctcag acaatgcatt gaacttcttc ctctgctcaa
      781 aaatatacct gagatcaggg atgtcataaa cgaggcagtg gaacagtttt caaaagaaag
      841 ttcggacagt gcagaaaaaa gaaagctcaa ggatcttctg aagaaaaagt ccgggagtaa
      901 gaaatgaact gtaaaatctt cagctttttg ctcactcagt cagtctaatg aaaaacagtt
      961 tagagaggtt tttatgaaaa aaaaatggat gatttttact gcggcggcac tgcttgcttc
     1021 ggcaggctta agtgcggaaa atattaccct gcagattaaa aaagaagagc tgcgtgaaga
     1081 gaatggaaag attctttttg aaccggacag aagggtgatg agagcaaagt atgatactta
     1141 tgtgtttgac aaaaagaact ttgaatccgt tgcaggcaaa aaagacaaga tgcccggatc
     1201 gttcaaattg aacatagaaa gcaagcctgg gacagtaaaa tcagtgaatc caaaggaagg
     1261 agctgcacct cagggaggaa ttaaaaccag atattttgaa gcaactgcat tgaagatcag
     1321 taaataatct tacttactca ccttacgcag gtggggcgtt tttggcagag agcctaccgg
     1381 cggttttaag ggctctgccc ttaaaaatcc cgtttttact gatccattag attttactaa
     1441 tgctttctga aaattacagg atctagatag catatcgtat ttgcgtaacg tgagtta
//
LOCUS       DAOQGJ010001217         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64746_length_1497_cov_3.230929, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001217 DAOQGJ010000000
VERSION     DAOQGJ010001217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64746_length_1497_cov_3.230929"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PK683_16545"
     CDS             <1..727
                     /locus_tag="PK683_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10108.1"
                     /translation="YFPSNGDDIDEEIFYSKLNSRKARLFFETQFLLAKHSYLFLGLR
                     ITQEQIVFRLNEMFIYPLVRMKNFLNEIFSEKRKMMSLQETVNGFLSEFKRGERKDPN
                     SPPVFGEKECLDEDRPHPIPETVYTSTREIIKLAQEKNIKLVFVVSPIDIETAYCSQQ
                     GKLHRLYTYSRAVRKFLEEEKVDFIDMNDYAVYMMDGEERFNVRPFYIIGDGHYTKAG
                     NSWMSAVLLLKLRQILGEDTDAF"
     gene            717..>1497
                     /locus_tag="PK683_16550"
     CDS             717..>1497
                     /locus_tag="PK683_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNH10109.1"
                     /translation="MLFNSLDFFIFLAVLLPLFFAVNEKRQRIVLFWFSLFFYGSLKT
                     VFVPLIFISFAATWHSSLKIQESSGIFRKKMYLLYCLAVNLGILIFFKYTDFFLGAWN
                     DFLHISGSAGSDSKMLGLVLPLGISFYTFQAVAYAVDVYRNKIAAENSFFRFSLFLLF
                     FPQLVAGPIMRAEDLMHQFTGKKTFSSADFSAGATLMAWGFFKKTIVADPIGTAISPV
                     FQNPELYDSASCFLAAFLFAVQIYCDFAGYSDIAIGIGRILG"
BASE COUNT          393 a          293 c          328 g          483 t
ORIGIN      
        1 ttattttccg agcaatggcg acgacattga cgaagaaatt ttttattcca aactgaacag
       61 ccgcaaggca aggctttttt ttgagactca gttccttttg gcaaagcaca gctatctttt
      121 tcttggactg aggattacgc aggaacagat cgtctttcgt ctgaacgaaa tgttcatcta
      181 tcctctggtc cgtatgaaga attttctcaa tgagatattt tctgagaaaa gaaaaatgat
      241 gagtcttcag gaaactgtaa acgggtttct gtccgagttt aagagggggg aaaggaaaga
      301 tcccaattcc cctcccgtgt tcggagaaaa ggaatgtctc gatgaagaca gacctcatcc
      361 aattcctgag actgtatata catccacaag agaaatcatt aaacttgccc aggaaaaaaa
      421 tataaaactg gtttttgtag tgtcgccgat cgacatagaa actgcgtact gctctcagca
      481 gggaaaactt cacagactct atacttattc gagagctgta agaaagtttc tggaagagga
      541 aaaagtcgat ttcattgata tgaatgatta tgcagtgtat atgatggacg gggaggaaag
      601 gttcaatgtc aggccctttt atatcatagg ggacggtcat tatacgaagg caggaaacag
      661 ctggatgtct gctgtccttc ttctgaagct ccgtcagatt ctgggagagg atacagatgc
      721 tttttaattc tcttgacttt tttatatttc ttgcggttct gctccccctg ttttttgcag
      781 tcaatgaaaa aagacagagg atcgttcttt tctggttttc acttttcttt tacggatctc
      841 tgaagacagt attcgttccg cttattttca tatcttttgc ggctacctgg cattcatcat
      901 taaaaattca ggaatcttca ggaatcttca ggaaaaaaat gtatctgttg tactgtctgg
      961 cagtgaatct gggaattctg atattcttta aatacactga ttttttcctg ggggcctgga
     1021 atgattttct gcatatttca gggtcagccg gatctgattc aaaaatgctc ggcctagtgc
     1081 ttcctctggg aatttctttt tatacatttc aggcagtggc ttatgccgtg gatgtgtaca
     1141 gaaacaagat tgcggctgaa aacagtttct tccggttttc tctgttcctg cttttctttc
     1201 ctcagcttgt tgcaggaccg attatgagag cagaagatct gatgcatcag tttaccggaa
     1261 aaaagacctt cagttcagcc gacttttcag ccggtgcgac gctaatggcc tggggttttt
     1321 tcaagaagac aattgttgct gatccaattg gaactgccat ttctcccgtt tttcaaaatc
     1381 cggaactgta tgattcagca tcctgttttc ttgcagcctt tctttttgca gtgcagattt
     1441 actgtgattt tgccggttac tccgatattg ctatcggaat aggaagaatc ctcggat
//
LOCUS       DAOQGJ010001218         1496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64822_length_1496_cov_3.171409, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001218 DAOQGJ010000000
VERSION     DAOQGJ010001218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64822_length_1496_cov_3.171409"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1020
                     /locus_tag="PK683_16555"
     CDS             205..1020
                     /locus_tag="PK683_16555"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH10110.1"
                     /translation="MHYIVDSFYNIVSYLMFFDQDASFAVTTAESYLKVFNGPALQFK
                     IRPGDRIPEGGAAFKAVQTGQFQVQLVPKEVYGIPFKSYAVPILEEGKVCNCILMGRS
                     IEKGDNLFNVSRQVSDALEKIFQSHTCLSQEIQKSVLMNSETMDGIKSAGEITRETDK
                     MLKFIQTISSRTNILGLNASIEAARAGGESGRGFAVVADEIRNLSLSTSDSVKSITVK
                     ISEIKHNMDKVSSNSNTINELFKAQVTALHKISDNLRTLSETVRNLESMAESL"
     gene            1151..>1496
                     /locus_tag="PK683_16560"
     CDS             1151..>1496
                     /locus_tag="PK683_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10111.1"
                     /translation="MNQEQQEKLRHLAGIIRRMPVKDARWILTDLESDLQFNREFLKY
                     LAKHSFQPNELFDLLSPEEFKSLFSKVPVPALQKLMSENPEKKNLFETALGKTKVSDI
                     LSKKLSAGEEDNY"
BASE COUNT          472 a          266 c          311 g          447 t
ORIGIN      
        1 agcagcggca tccatacttg cgagattaga aatctgagta ttatatctaa ctgtgttaat
       61 gcagattttt tctaatcaga gagcagatat tctttttttt aaattctttt attattgaat
      121 aaaacaagac tttctttgca ctggtcctta aacagacaga gtcagtgctg ctatgcggat
      181 tcttgattta aacggtaaaa agtaatgcat tatattgtcg attcatttta taatatagtt
      241 tcttatctta tgttctttga tcaggatgcg tcattcgctg tgaccacagc agaatcctat
      301 ctcaaggttt ttaacggtcc cgcactccag tttaaaatca gaccaggtga ccgcattccg
      361 gaaggaggtg ctgcatttaa ggcagttcag acaggacagt ttcaggttca gcttgtacct
      421 aaggaggttt acggaatccc ctttaaatcg tatgcagttc ctattttaga ggaagggaaa
      481 gtttgcaact gtattttaat gggaaggagt attgaaaaag gagacaatct gtttaatgtt
      541 tccagacagg tttctgacgc attggaaaaa attttccaga gtcatacctg cctttctcag
      601 gagattcaga aatccgtact gatgaattct gaaacgatgg atgggataaa atctgcaggc
      661 gagattacca gagagacaga taaaatgctc aaattcatac agacaatttc atccaggaca
      721 aatattctgg gattgaatgc atcgattgag gctgcccggg caggaggtga atcaggacgc
      781 ggattcgctg tggtggccga tgagatcaga aatctttctc tgtccacatc tgattcggta
      841 aaaagcatta cagtgaaaat aagcgaaatc aagcataata tggataaggt ttcgtctaat
      901 tcaaatacaa tcaatgaact ttttaaggct caggtaactg ctcttcataa gatttccgac
      961 aatctgagga ctttaagtga aacggtcagg aatctggaat ctatggccga aagcctttag
     1021 catctgtgct ttattaatct gcatccctaa gcaaaaaaag atatattttc caatgattca
     1081 aatttaaata tgcctaaact ccggaagtct gctgatcagt gattcaggag actttcagta
     1141 aagggattat atgaatcagg aacagcagga aaaacttcgt catttggcag gtattatcag
     1201 gagaatgccg gtaaaggatg ccagatggat tttgactgat cttgaatctg atcttcagtt
     1261 caacagggaa tttctgaaat atcttgcaaa acacagtttt cagccgaatg aactttttga
     1321 tcttctttca ccggaagaat tcaagtcgct attcagcaag gttcctgttc ctgcacttca
     1381 gaaactgatg agtgaaaacc ccgagaaaaa aaatcttttt gaaacagctt tgggaaaaac
     1441 aaaggtttcc gatattctct caaaaaaact ttctgcaggt gaggaagata attact
//
LOCUS       DAOQGJ010001219         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_64975_length_1494_cov_3.715775, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001219 DAOQGJ010000000
VERSION     DAOQGJ010001219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64975_length_1494_cov_3.715775"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..829)
                     /gene="cobC"
                     /locus_tag="PK683_16565"
     CDS             complement(248..829)
                     /gene="cobC"
                     /locus_tag="PK683_16565"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HNH10112.1"
                     /translation="MKKTILFLRHPAPKIPLGFCYGQTDLEIAEPEKLSPSVSEFELI
                     FSSPLQRCIKAAQILFPKNEIITDERLKELNFGEWENRKWDDVPKNELDEWSKNLLSF
                     RPGGSESFQELSERVNAFLQELLQRKEEKILVLSHAGTMRAALSHIMNISLEKTLNFS
                     LGYFHCAEYAFAEPKRRLLFWNLDFHSFLKEKH"
     gene            complement(829..>1494)
                     /locus_tag="PK683_16570"
     CDS             complement(829..>1494)
                     /locus_tag="PK683_16570"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH10113.1"
                     /translation="EFNRKDLTEAGFKNSEVFPFALNFYNYKKLGKNTDSKKKILFVG
                     RIAPNKKQTDLLKAAHIFRKYTDIPFEFRLVGFTSRESILYRHELDWLISSYSLSENV
                     RILEFASQEDLFREYSEADAFLCLSEHEGFCVPLLEAIYYDIPVAAFDAGAISETLGK
                     SGILFKEKNYLLISSILEKLLTDSGFREGILRSQRQRLEEYKVSSNPEILMQKLLDSW
                     DII"
BASE COUNT          423 a          293 c          307 g          471 t
ORIGIN      
        1 ctatttagct tctgatgaaa gataatttct tgattttttc aacaagtcta ttatatctca
       61 gaagaaggaa gacttaaaag caggacattc aggaaacgaa aacatctaaa ctgattattg
      121 atccgctttt aaagcaatgt gccgggatga taatattctt tttaaatcag tgagatgttc
      181 ttagagagta tttgcagaaa gacaatttca gcgaaaacaa gccttggtat gcattcagga
      241 tgtgttttca gtgcttttct ttcagaaaag aatgaaaatc caaattccag aagagcagtc
      301 gacgtttggg ttcggcaaat gcatactctg cacagtgaaa atatcccagt gagaagttta
      361 aagttttttc taatgatata ttcattatat gcgaaagtgc ggctctcatc gtgcctgcat
      421 gagacagaac aaggattttt tcctcttttc tctgaagaag ttcctgaaga aacgcattca
      481 ctctttcaga cagctcctga aaactttccg aaccgccggg gcggaaactg agcaggtttt
      541 tcgaccactc atcaagttca tttttcggaa catcatccca ttttctgttt tcccattcac
      601 cgaagttcag ttctttaagt ctttcatctg tgatgatttc attctttgga aagagaattt
      661 gagccgcctt gatgcatctc tgcagcgggc ttgagaaaat cagctcaaat tcagatacac
      721 ttggagaaag tttttccggt tccgctattt caagatccgt ctgaccgtag cagaaaccga
      781 gcggaatctt cggagccgga tgacggagaa aaagaattgt ttttttcatt atattatatc
      841 ccaagagtcc agcagcttct gcataagaat ttccgggttt gaggaaactt tgtattcttc
      901 aagtctctgt ctttgcgacc tcaggatgcc ttctctgaac ccgctgtctg taaggagttt
      961 ttcgagtatt gatgaaatca gaagatagtt tttttccttg aaaaggattc cgctttttcc
     1021 gagtgtttct gaaatggcgc ctgcatcgaa tgctgcaacc ggaatatcat agtaaattgc
     1081 ctcaagaagg ggaacacaga agccctcatg ttcgctcaga cagagaaatg catctgcttc
     1141 ggaatattct ctgaaaagat cctcctgtga ggcaaattcc agaattctga cattctcgga
     1201 cagtgagtag gatgagatca gccagtccag ttcatgtctg tacagaatgg attctctgga
     1261 ggtgaatccg accagcctga attcaaaggg gatatccgta tattttctga aaatatgagc
     1321 agccttcaga aggtctgtct gctttttgtt gggagcaatc cttccgacaa aaagaatttt
     1381 ctttttgctg tctgtatttt ttcctaattt tttataatta taaaaattca gtgcaaaagg
     1441 gaaaacttcg gagtttttga acccggcttc agtcagatcc tttctgttga attc
//
LOCUS       DAOQGJ010001220         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65021_length_1494_cov_2.107019, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001220 DAOQGJ010000000
VERSION     DAOQGJ010001220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65021_length_1494_cov_2.107019"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..514)
                     /locus_tag="PK683_16575"
     CDS             complement(152..514)
                     /locus_tag="PK683_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10114.1"
                     /translation="MNPQNLDYIRYLLDLCDALLEKEEILQRDTDSFNREVILVKDNI
                     PPKEIPEIETVQQIMIKESFLKKGLNIFTWIQFFSVKLLSRMLYGDIEKTEILKDQLR
                     EIRNQLSHILFKYQGRSS"
     gene            complement(519..836)
                     /locus_tag="PK683_16580"
     CDS             complement(519..836)
                     /locus_tag="PK683_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10115.1"
                     /translation="MGYIYSIGGDMMHRIEIEAERKILILDGAMAAMIQHRFSLRELM
                     ELISHIEDGKTLIAAKSKFEAEKNLHHSEQAMLLTECAFFRQGSVSEIRKPIPSVIMT
                     LQG"
     gene            complement(1032..>1494)
                     /locus_tag="PK683_16585"
     CDS             complement(1032..>1494)
                     /locus_tag="PK683_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNH10116.1"
                     /translation="EGIHKSGMKVSEVVAKAKDKNSTFRLSGFGHRVYKNFDPRAKII
                     KKACDAVLNKLGIKDPLLDIAKELEEAALKDQYFVDRKLYPNVDFYSGIIYRALGIPT
                     NMFTVMFGMGRLPGWIAQWKEMIESPDMKIGRPRQIYVGPTVTNYDDVKKK"
BASE COUNT          375 a          311 c          280 g          528 t
ORIGIN      
        1 gcacttctta tctggtcggt gacgaaatcc acaaatttct gatctgatgc cattttggtt
       61 tcctttggaa aggctgctct gtaaaatctg aatcttttcc atatctaaag acttttatta
      121 tttcaagaat ctaaatgtca agtgcctgaa gtcaactgct tcgtccctga tatttaaaaa
      181 gaatatggga aagctgattt cggatttctc ggagctgatc ctttagtatt tcagtctttt
      241 caatatctcc gtataacatt cggctcagga gcttcactga aaaaaactgg atccaggtaa
      301 aaatattcag tccttttttt aaaaaagact cttttatcat tatctgctga actgtttcga
      361 tttctggaat ttctttcgga ggaatattgt cttttactaa aatgacttcc ctgttgaatg
      421 agtcagtatc tctctgaaga atctcttctt tttccagcaa tgcatcacat aagtccagta
      481 aataacggat atagtccaga ttttgcgggt tcattatttt atccctgcaa tgtcattatg
      541 acggacggaa tcggttttct gatctcagat actgaaccct gccgaaaaaa tgcgcattca
      601 gtcagcagca tagcctgttc gctgtgatgc agattttttt ctgcttcaaa ttttgatttt
      661 gccgcgatca gtgtttttcc atcttcaatg tgcgatatca gctccatcag ttccctcaag
      721 gagaacctat gctgtatcat tgctgccatt gccccgtcca gtattaagat cttcctttct
      781 gcttcgattt ctatcctgtg catcatatct cctccgatac tgtaaatata tcccattaaa
      841 atatgaggat aaaaagattt atgaagaatg caatcagttt tctatttact catatttcca
      901 atagtgaagc gttttatccg gggtttttga cagtggaaaa cggttgagaa gttttttcgg
      961 ctggcttgaa gaactaaagt gagtttgctc cccgacagat ttctctgtcg gagtgtgtat
     1021 tgatttttta attatttttt ctttacatca tcatagtttg tgactgtagg tcctacatag
     1081 atctgacggg gtcttccgat tttcatatcg ggagattcaa tcatttcctt ccactgagca
     1141 atccatcctg ggagacgtcc cattccgaac attacagtga acatatttgt aggaattcct
     1201 agagcacggt agatgattcc gctgtagaag tccacgttcg gatagagttt tctgtccacg
     1261 aaatactgat ccttcagtgc agcttcttcg agttcttttg cgatatccag aagagggtcc
     1321 ttgattccga gcttgttcag aactgcatca catgcttttt taatgatttt tgccctagga
     1381 tcgaagtttt tgtacaccct gtgtccgaaa ccggaaagac ggaaagtgga gttcttgtct
     1441 tttgcctttg ctactacttc actgaccttc attccgctct tgtgaattcc ttcg
//
LOCUS       DAOQGJ010001221         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65133_length_1492_cov_3.680585, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001221 DAOQGJ010000000
VERSION     DAOQGJ010001221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65133_length_1492_cov_3.680585"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..920)
                     /locus_tag="PK683_16590"
     CDS             complement(378..920)
                     /locus_tag="PK683_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10117.1"
                     /translation="MEKTERIIICDAGPIIHLDELNCLDTLDFQRILVPISVWKEILQ
                     YRNISSESIEGLEIIETAELPLEFLEICKLYDLHKGEAAAIFLSLELKNSILFTDDGA
                     ARLFAKSKNISVHGSCPSSQSILAIKICFSFQELFHSPQKKSPQDDLHPAGVHTWFFI
                     LIRSLRHIRHFDRLNAPQAQ"
     gene            complement(907..1101)
                     /locus_tag="PK683_16595"
     CDS             complement(907..1101)
                     /locus_tag="PK683_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10118.1"
                     /translation="MTEITVKVPNNLLNIAQKLVADGWFLDENEIFLLAFRNYLRTHS
                     DEIISSFIKEDIEWGLSGKD"
BASE COUNT          448 a          305 c          318 g          421 t
ORIGIN      
        1 tagcaggacc tgcacaagga acgattacag cgggagattt ggatggaacc ggtaatgtgc
       61 caagatttaa taattccttt ggaattacta cagacggaac cagtctgtat atggtcgaat
      121 tcaacaacaa caaggttcga aagattcagt agccgcaacc tctacttgat tcaggataca
      181 ttgtattgca ccatcagggc acactgtctg tgctctgatg gtcgggactg tccgacatat
      241 ccgctcctga agtgcagggc tgtgaaagcg ggatcttggc tgctgaacgg agtcgatggc
      301 ttcgagagcg tcgatggcgt cagccacctc ctctcagccg ctggacactg agaaaggcaa
      361 gggcactcag gttctggtca ctgagcctgc ggtgcattga gcctgtcgaa atgtcgaatg
      421 tgtcgaaggg agcgaatcaa aataaaaaac caagtgtgaa cacctgcagg atgcaagtcg
      481 tcctgcgggg attttttttg gggggagtga aacaattctt gaaatgagaa acagattttt
      541 atggcaagta tcgactgact gctaggacag cttccatgta cagaaatatt cttacttttg
      601 gcaaataatc ttgctgctcc atcatccgta aataatattg aatttttcaa ttccaaagat
      661 aagaaaattg cagctgcttc tcctttatga agatcataca gtttacagat ttccaagaac
      721 tctaagggca attctgctgt ttcaatgatt tcaagtcctt caatgctttc tgaagagata
      781 ttcctatact gcagtatttc cttccagaca gatataggaa ctagaattct ttggaaatca
      841 agcgtatcta aacaatttaa ttcatccaaa tgaataattg ggccagcatc acagattatg
      901 atccgttcag tcttttccac tcaaacccca ttctatatcc tctttaatga aagaagagat
      961 aatttcatca gaatgagttc ttaaataatt tctgaacgcc aaaagaaata tttcattttc
     1021 atccaaaaac catccgtcag caacaagttt ctgtgctatg ttcaataagt tattcggaac
     1081 tttgactgta atttctgtca tacatcctcc tttctctgac agtaccggtt atacagaaaa
     1141 aatcaactgt tttatatctg catattcgaa gcataggctc tagaccttac aggctcacca
     1201 accaagcctg cggtgcattg agcctgtcga aatgtcgaat gtgtcgaata gattaaaaca
     1261 aagaaaaaaa acaagtgtga acaactgcag gatgcgagtt atcctgcagg gatttttttt
     1321 gggagagagt gagtgttcgg agcgaatttg gcggggtaag caagggcagc ttcttcaatg
     1381 agcactacag attatcaaag tcctctcgca gtctctttgg aggcaattct actttttcat
     1441 agacgctatc taaggaaagc tctatatcca aagattgtaa gcgaaagttg tc
//
LOCUS       DAOQGJ010001222         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65143_length_1492_cov_3.155880, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001222 DAOQGJ010000000
VERSION     DAOQGJ010001222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65143_length_1492_cov_3.155880"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PK683_16600"
     CDS             <1..642
                     /locus_tag="PK683_16600"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH10119.1"
                     /translation="SVFRTDQFRSMRKVYSENLLIPSETHWLENTDASQEFFIDKNFR
                     IQEINADQPEDREKSIQISPKTGEYFSDSPVVHYKISLLAGKIMNLNQVTVPSADVAR
                     SIRFYQKLGLKLIVKNLPDYARFECTEGDSTFSVHKAEKLPEGEGTIVYFETQDLERL
                     TEKLILDGIVFETGPALQDWLWKEASLKDPDGNRIILFTAGENRKNPPWRLSS"
     gene            complement(706..1056)
                     /locus_tag="PK683_16605"
     CDS             complement(706..1056)
                     /locus_tag="PK683_16605"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNH10120.1"
                     /translation="MNKLFIFLLISFFTAGIMAHGGKKHGAGKKEMFQKMDQNKDGKI
                     SKEEWNAFHESRFTELDKNKDGSVTEEELAEFHKDKHKGKHKGKDKEKHSHKDKDKGK
                     DKDKDKEKDKDNDD"
BASE COUNT          424 a          311 c          296 g          461 t
ORIGIN      
        1 tcagtcttcc gaacggatca gttcaggtca atgagaaaag tttactcaga aaacctcctg
       61 attccatccg aaacgcattg gcttgaaaat acagacgcat ctcaggaatt ttttattgac
      121 aaaaacttca gaattcaaga aataaatgca gatcagccgg aagacagaga gaaatcgatt
      181 caaatcagcc cgaaaacagg tgaatatttc tcagacagtc cagttgttca ttataaaata
      241 tcacttcttg cagggaaaat tatgaatctg aatcaagtta cagtaccttc agcagacgta
      301 gccagatcaa tccggtttta tcagaagctt ggtctgaaac tcattgtaaa gaacctgccg
      361 gactatgcga ggtttgaatg cacggaaggt gattcgacat tttccgttca taaagcggaa
      421 aagcttccgg aaggagaagg aacaattgta tatttcgaaa cgcaggatct ggaaaggctg
      481 acagagaagc tgattctgga tggaattgtt tttgaaactg gacccgctct tcaggactgg
      541 ctttggaagg aagcatcctt gaaagatccg gatggaaacc ggatcatcct gttcactgca
      601 ggagagaacc ggaaaaatcc tccctggagg ctgagttctt gagttctgca gattcaaact
      661 gcagaactta caatcaggat ttagactgca gactgacagt gaaaatcagt catcgttgtc
      721 tttgtctttt tccttatctt tatctttgtc ctttcccttg tctttgtctt tatggctgtg
      781 tttttccttg tcttttccct tatgctttcc tttgtgctta tccttatgga attcagccag
      841 ttcttcttct gttacggaac cgtctttgtt tttgtccagt tcagtaaatc tgctctcatg
      901 aaatgcattc cattcttcct tggaaatttt tccgtctttg ttttggtcca ttttctggaa
      961 catttctttt tttccggcac cgtgtttttt gccgccatga gccataatac cagctgtaaa
     1021 aaagctgatg agaagaaaga tgaataattt gttcatactg tatccttttg tattgagcag
     1081 aggaaaatcc cctgcacttc tacactgtta gacttaactt aaatgaaatt gttcactcat
     1141 atcttcattc tgccgagtcg gaataatgcg acttttaaat accctcaaag cccgtctgct
     1201 gcctatgact gcgaggatcg ctttaagagg actaagaaat gacagcccaa tcaccggatt
     1261 ggaaaccgac taaactcccg caagccagga agcagtgaag cggctttttt ttctgattca
     1321 ttgtaatagt gtaaactgct agattcttcc agtgcttagg aaaagttaca agttctattc
     1381 attatattct cttgacgcaa gatgagtcat taaataacaa atctgcatta ataaacatcc
     1441 actgacttga aagaggatat ttccccaaga cgtcttcgag acagcttctg gt
//
LOCUS       DAOQGJ010001223         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65186_length_1492_cov_1.815588, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001223 DAOQGJ010000000
VERSION     DAOQGJ010001223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65186_length_1492_cov_1.815588"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          480 a          350 c          278 g          384 t
ORIGIN      
        1 aaaattttaa cacccaaaat ccccctcctc cacccatttc cccaaaaatc caagacacgc
       61 acggaaaaaa accgcatccc gcattctatg cagaatcatt aaataattat aaaatattca
      121 tttatatata gaaatatccc acactcaata catatcataa catcattaaa aaatattctt
      181 attcagcaga tgctgatcca atccgcagac aatgacaatc acgcccgtta aaaaaacagc
      241 ccgatgaact gtaatccgta taatccggac aagttcactc gcccagccga atttaatccc
      301 gcagagatcc tctctcgctc aacaaattat ttttaaagat ctttgcgtat tttacaggtt
      361 ttcatgacag tcgaaggtct gctgatataa agtcgaacag ctttgcagat tctattaagc
      421 ggttctttgc accgaaatca gaaaagaatt ctttcttaac tgagccatag tcgttcaaaa
      481 gggacgatag tcccttgaaa cctccggtag gattaactca gctcgcataa gcgagctgca
      541 ggttttttta gaaaaattaa aaatagagtt gttgaaacta atttttcaac aactctaaaa
      601 agtacaagcc tgaatcttgg ataagcccga ttattttcaa aaagaagtat aaatcatttc
      661 ataattacag aatatccatg aatgcaggat tgaaccgatc tgtagggcca gggcagacat
      721 gaagctaaag atctgcatcg caggtcgaag tcctgcgtgt ccttcgctga aaacagcttt
      781 ccctctgaca gagacgactg ccggtgactt ctcgccgctc cggcggatga gacatcggca
      841 gactgcaaaa acagaaatcg gatcggcttt aggggaaaat aaatgattcc tatgcagaag
      901 ctgaaagaca ggatatgtca aggtctgcag cagaccgcag aacagaatac aactgacgag
      961 tccagcggca tcaattgatg ccgccctgca gtgcggcatt cagcgatgct gcaaatttaa
     1021 tcgccgtcaa tgactgactt catgagaatt tcattagaaa tttgaggaat atcattcccg
     1081 gcacctgtca attcagcggt ccttctgaaa tttgatcgga atcacatatc cgtctgaacg
     1141 gaacagttct ttgatgccgg aaaatgcaaa acacattgct ttcaagcgcc cgttctaatt
     1201 gtatgagcct gattcttctc ataaattaat tcttgcagcc ttgctcttcc tgtgcttctg
     1261 cagaaattat gcatcctgcc tcgcctttct gaagtcagga aacagatgaa tcccgaaatg
     1321 tgagcactga aaggccgaat ctcctggaca aaatcatttt ccgtattccg catcgagaat
     1381 tggaatggct ctgaatcagt acacagaaat tcctgacaga ccctgaacag aagatcctcg
     1441 gataataatt tcacacaggc actgcagaca gaactatcgg cagtctcaat ga
//
LOCUS       DAOQGJ010001224         1491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65227_length_1491_cov_2.961003, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001224 DAOQGJ010000000
VERSION     DAOQGJ010001224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65227_length_1491_cov_2.961003"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PK683_16610"
     CDS             <1..404
                     /locus_tag="PK683_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10121.1"
                     /translation="SKKWTIISRTGLSNMKTKLFVSLIMMTVFGLSITAGDKKMKSIT
                     MHDLMEDHFEDAEKAFKRKKDKTKLEALLKILPDLAPEDDKKEWTAIVNTHLESGELL
                     KSCKACHSKFKKSYKKSYKKRLVEVPEDLLE"
     gene            complement(769..>1491)
                     /locus_tag="PK683_16615"
     CDS             complement(769..>1491)
                     /locus_tag="PK683_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10122.1"
                     /translation="RLIQMTFGDIQIFKYTVFLFIVCFFILNCTFSERQLYTDEYETV
                     ISDGFIRVSKRKMIQKADILKSVSYDFPEKESVIPDKEFFSYAVQGLKKEMVSIESLS
                     LQIENLTLEANSEKSIRKYFFPYYSDSVRFKTVIVSKLSLRKDSLCFQGESSVFESSE
                     RQLNLKVYSFLPVPAWMELLRYASGSVYGIFLIFVPVPYHYYNNGREAGEYSELTAGE
                     YKTHILEAVRNAKEKNLFRKCE"
BASE COUNT          481 a          334 c          259 g          417 t
ORIGIN      
        1 catcaaagaa atggacaatt atttccagaa ccggtctaag taatatgaag actaaactgt
       61 ttgtttcact tattatgatg actgtcttcg gcctgagcat aacagccgga gacaaaaaaa
      121 tgaaatcgat cactatgcat gatctgatgg aagaccattt tgaagacgca gaaaaagcat
      181 tcaaaagaaa aaaggacaag acaaaactcg aagcccttct caaaatcctt cctgatctcg
      241 caccggaaga cgataagaaa gaatggactg ctatcgtaaa cactcatctg gaatcgggtg
      301 aactgctgaa atcctgcaag gcctgccaca gcaaattcaa aaaatcttat aaaaaatcct
      361 ataaaaaaag actagtggaa gttccagaag atcttctgga atagaggcaa ttttggcttt
      421 ggtgtctaaa ggcaaaattg ctgcagactg tgcttaaaag cattataaaa tgaggctcac
      481 gcggagacgc ggagcaccgc tttctcgcat ctaaaacctt tccggaccaa tgcgaccggg
      541 ctttcagctt ctgtcaagtt cctgcgctcc gctatccgca gaaacttgac accgccacaa
      601 cttgccgtga tgcctgtcga atccatccct ttcgcgatgg cggttttata aaaggaatct
      661 tcatcattgc aaatatcctt cagaactcca tactctgaac cctgaactca aattattttt
      721 catgataaaa tatccccttt gatcgctgct cgctattctc tatccgcact attcgcactt
      781 cctgaacaga ttcttttctt ttgcattgcg gactgcttct aagatgtggg tcttgtattc
      841 gcccgctgtc agttctgaat attctcctgc ttctcttcca ttattataat aatgatatgg
      901 aactggaaca aaaatcagaa aaattccata aacagaacct gacgcatatc tcaaaagttc
      961 catccaggcc gggacaggca aaaaactgta aaccttaaga ttcaattgcc gttcactgga
     1021 ttcaaaaact gaactttctc cctgaaagca aagagaatct tttcttaatg ataatttaga
     1081 tacaatgacc gttttaaatc ttactgaatc tgaatagtac ggaaaaaaat actttctaat
     1141 gctcttttcc gagttcgctt caagagtcag attttcaatc tgcagagaca gactttcgat
     1201 tgacaccatt tcctttttta gcccctggac ggcataggag aaaaactcct tatctggaat
     1261 gaccgattct ttctcaggaa aatcataaga gactgatttc agaatatctg ccttctgaat
     1321 catcttcctt ttactgactc gaatgaaacc atcgctgatt actgtctcat attcatccgt
     1381 ataaagctgc cgctccgaaa atgtgcagtt cagaataaaa aaacatacta taaaaaggaa
     1441 aactgtatat ttaaagattt ggatatcccc aaaagtcatc tgaataagcc t
//
LOCUS       DAOQGJ010001225         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65366_length_1489_cov_2.468619, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001225 DAOQGJ010000000
VERSION     DAOQGJ010001225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65366_length_1489_cov_2.468619"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK683_16620"
     CDS             <1..389
                     /locus_tag="PK683_16620"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNH10123.1"
                     /translation="LSIGIGVFQSRPEPQETVLSAERNVTEILRRSCFDCHSSEVRWP
                     WYSYIFPLSSLIAHHIKEGREELDFSVWDSLSVKKRNSLADKIIEEIDKNEMPLKSYT
                     FMHREAVLSSEDREILKNWAKGFMDE"
     gene            382..>1489
                     /locus_tag="PK683_16625"
     CDS             382..>1489
                     /locus_tag="PK683_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNH10124.1"
                     /translation="MNREEILENRRFMSLHSEFSSWKFQLKNRIRAKDLHRYYILTEN
                     ELAGIEKTVRLNVGTVPYYMLLSDPFDPACPIRRMIIPNEMETFRSPEESPDPLHEEI
                     LSPVKGLTRMYPDRVLLFSNQECSVYCRHCMRGRKVSYNEERVTEDDLERCFHYIREN
                     PEISDVVISGGDPLNLSDSRIEFILKSLEEIPSVKICRIGSRNPVTLPFRISGELCEI
                     IERYNTDRLSIFCNTQFNHAKECTDEAKTAVLRLLKAGVSVGNQCVILKGINDSGEVM
                     LSLHRKLLEMRIRAYYMYDPELIPGSRGFRTPLWKGVEIIEYMRGKIAGFGIPQFVND
                     LPGGGGKITMSPDWYIGFHRKSRTHVFRSAVRGTF"
BASE COUNT          453 a          286 c          358 g          392 t
ORIGIN      
        1 cattatcaat cggaattgga gtctttcaga gcaggcctga accgcaggag acagtgcttt
       61 ctgctgaaag aaatgtgact gaaatactga gaaggtcctg ctttgactgt cattcctcag
      121 aggtcagatg gccctggtac tcctatatat tccctctgtc cagtctgatt gctcatcata
      181 taaaggaagg aagagaagaa ctggattttt cagtatggga ttctctgagt gtcaaaaaaa
      241 ggaattctct cgccgacaag attattgagg aaattgacaa gaacgaaatg cctttgaagt
      301 catatacatt tatgcacaga gaggcagttc tttcctcgga agacagggag atcctgaaga
      361 actgggcgaa aggattcatg gatgaatagg gaggaaatac tggagaacag acgtttcatg
      421 tctcttcact ccgagttttc ttcctggaaa tttcagctga aaaacagaat cagagcaaaa
      481 gatctgcaca gatattatat cctcactgaa aatgaattgg caggaattga aaagactgtc
      541 aggctcaatg tggggacagt accttattat atgcttcttt ccgatccttt tgatccggcc
      601 tgtccgatca gaagaatgat aatcccgaat gaaatggaaa cgttccgttc gcctgaagag
      661 tcccccgatc ctctccatga agaaattctt tcgccggtca aaggactgac caggatgtat
      721 cctgacaggg tccttctgtt ttcaaaccag gaatgttctg tttactgcag acactgtatg
      781 agaggaagaa aagtatctta taatgaagaa agagtgacag aagatgattt agaaagatgt
      841 tttcattata tcagagaaaa tcctgaaata tcagatgtag tcatttccgg cggagatccg
      901 ctgaatctga gcgattcaag aatagagttt attctgaaaa gtctggaaga gatcccgtcc
      961 gtaaaaatct gcaggattgg aagcagaaat ccagtcactc ttcctttcag gatatcggga
     1021 gaactctgtg aaatcattga acggtacaat acggacaggc tttcgatatt ctgcaacact
     1081 cagttcaatc atgcaaagga atgcacagac gaggcaaaga cggctgttct cagactgctc
     1141 aaagcagggg taagtgtggg caatcagtgt gtgattctga aaggaataaa tgattcaggt
     1201 gaggtgatgc tgagtcttca cagaaaactt ctggaaatga gaatccgcgc ttattatatg
     1261 tatgaccctg aactgattcc cgggtctaga ggtttccgta ctccgctctg gaaaggggta
     1321 gagatcatag aatacatgag gggaaaaatt gcaggttttg gaattcctca gtttgtgaat
     1381 gatctgcccg gcggcggcgg taaaattaca atgtctcccg actggtacat tggatttcac
     1441 agaaaaagca gaactcatgt attccgttcc gcagtcagag ggacttttc
//
LOCUS       DAOQGJ010001226         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65525_length_1487_cov_2.868017, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001226 DAOQGJ010000000
VERSION     DAOQGJ010001226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65525_length_1487_cov_2.868017"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK683_16630"
     CDS             <1..487
                     /locus_tag="PK683_16630"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004491673.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNH10125.1"
                     /translation="QHGVPLEEFVEAFTFFKFEPNGMVQGNSHIKMSTSVIDYIFREL
                     AITYLGRYELGQVLPEDLRGDEVGPGESPKSEIENDEIQEPNPPSLISGSAAKETKPI
                     SMTQVMTSKAAVGASMNKREAAIIQGYTGDSCSECGSFQMVRNGSCLKCNSCGTTTGC
                     S"
     gene            504..938
                     /gene="dut"
                     /locus_tag="PK683_16635"
     CDS             504..938
                     /gene="dut"
                     /locus_tag="PK683_16635"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNH10126.1"
                     /translation="MKISVQKIHSDSVLPELKTVGSAGYDLFAYAKDGMTAEPMKVCL
                     VPTGLRFEIPSGFDFEIRPRSGLSTKHMLLIPNSPGTIDSDYRGELFIPILNLSKEVF
                     QITHSMRIAQLLIRKTIHTEWIVSDSLLDTERGAGGFGSTGI"
     gene            1024..>1487
                     /locus_tag="PK683_16640"
     CDS             1024..>1487
                     /locus_tag="PK683_16640"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNH10127.1"
                     /translation="MPSQPGNKFIEKFLFIPENANPVKRKLILGVRVLYASLKRFLED
                     NCLMVASGISYTTIVSLVPTLTVALALLTISSGYSEKQELISETITTFLQKNEIKIDI
                     TPYLDTLKDIVSSATQIGAVGFIVLIFSATAILRTFEAAFNQIWRVKVQRPFS"
BASE COUNT          423 a          306 c          335 g          423 t
ORIGIN      
        1 tcagcatggg gttcctctgg aagaatttgt agaagcattt acattcttca aattcgaacc
       61 gaacggaatg gtgcagggaa acagccatat caagatgagc acttccgtaa ttgattacat
      121 tttccgagaa ctggcaatta cttatctcgg aagatatgaa ttgggtcagg tgcttcccga
      181 ggatctgaga ggagatgagg tagggccggg ggagtccccg aaaagcgaaa ttgaaaatga
      241 tgaaattcag gagccgaatc ccccttcact gatcagcgga tcggcggcaa aggagactaa
      301 gcctatctct atgactcagg tgatgacatc gaaagcagcg gtcggagcat caatgaataa
      361 gagagaggct gcaattattc agggatacac tggagattcc tgttcagagt gcggatcttt
      421 tcagatggtg agaaacggtt cctgtctgaa atgcaactcc tgcggaacaa ctaccgggtg
      481 cagctgatcc ttatatgagt ctgatgaaaa tttcagttca gaaaattcat tcggactcag
      541 tcctccctga attaaagaca gtcggctctg caggatatga tctctttgca tatgcaaaag
      601 atggtatgac tgctgagccg atgaaagtat gccttgttcc tacaggactg cggtttgaaa
      661 ttccttccgg atttgatttt gaaatcagac ccaggtcggg actttccaca aaacatatgc
      721 tcctgattcc aaattcaccg ggaacaattg attccgacta cagaggagaa ctgtttattc
      781 cgattctgaa tctcagcaag gaagtgtttc agattactca ttctatgaga attgcacagc
      841 ttcttataag gaaaacgatc catacagaat ggatcgtttc cgacagtctt ttagacactg
      901 aaagaggtgc gggcggattc ggttctaccg ggatttaatt tcagcaattc atttctctta
      961 tccgtcctta ttgaaaattt aattgctttc tattcagcct ttataaaatg aatagtgttc
     1021 accatgccaa gtcagccagg aaacaaattt atagaaaaat ttctgttcat tccggaaaat
     1081 gcaaatccgg ttaagagaaa actcatactt ggagtcaggg ttctttatgc ctccctgaaa
     1141 agattcctgg aggataactg cctgatggtt gcctccggga tttcctatac tacaattgtt
     1201 tctcttgtcc ctacgcttac tgttgctctg gctcttctga caatcagttc gggttattct
     1261 gaaaagcagg aactgatttc tgaaacgatc actacatttc ttcagaaaaa tgaaattaag
     1321 atagatatca ctccctatct ggacactctc aaggatattg tgagttctgc gacccagatc
     1381 ggggcagtcg gatttattgt tctgattttt tctgcaactg caattctcag aacattcgag
     1441 gctgcattca atcagatctg gagagtgaag gtgcagaggc cattttc
//
LOCUS       DAOQGJ010001227         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65540_length_1487_cov_2.349860, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001227 DAOQGJ010000000
VERSION     DAOQGJ010001227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65540_length_1487_cov_2.349860"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PK683_16645"
     CDS             complement(<1..335)
                     /locus_tag="PK683_16645"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNH10128.1"
                     /translation="MVYQQRRSERVSYKNFSDYSVSFTADDKTYTGLLGNISDGGMCA
                     ILPAEFPAEIGGLLKGELKYKPYSDIYPISTKIVWKANYLLKDEPKIMIGMEFAEKFV
                     LPEYLIALS"
     gene            complement(374..1279)
                     /gene="pyrB"
                     /locus_tag="PK683_16650"
     CDS             complement(374..1279)
                     /gene="pyrB"
                     /locus_tag="PK683_16650"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNH10129.1"
                     /translation="MFIKSCYQSTYFIISKTNRMKELLHSGQAFGLLHGKLLASLFFE
                     ASTRTRLSFESAMERLGGRVISTVGFQFSSISKGETLYDTMKMIEAYADIAVIRHPVE
                     GSSRIAAGAVKMPVINAGDGAGQHPTQALLDMYTIKSEKGTLDNITVGFVGDLKYGRT
                     IHSLIRLLHHYKIHLYLISPEELSLPDNYKKDPSNSLLTFEETRDIKAIWDCDVVYVT
                     RIQEERFPDHREYEKLKETYKVNKELILASRKNTTVLHPLPRVSELSTDVDDLPNAAY
                     FRQAEYGVAVRMTLLCLCLGVEIPE"
BASE COUNT          403 a          308 c          321 g          455 t
ORIGIN      
        1 atactgagag caattaagta ttccggcaga acaaacttct ctgcaaactc cattcctatc
       61 ataatttttg gctcatcttt taaaagataa ttcgctttcc agacaatctt agtactgatc
      121 ggatagatgt ctgagtaagg tttgtactta agttcaccct taagaagtcc gccgatctct
      181 gcagggaact ctgcaggaag aatggcgcac atccctccgt ccgaaatgtt tcctaaaagg
      241 cctgtatagg ttttatcgtc tgcagtaaaa ctgacagagt agtctgaaaa gtttttgtag
      301 gaaactcttt ctgatcttcg ctgctgatac accataaatc tacaattttc cttcagaata
      361 ttatttcaat caattattcc ggaatctcaa ctcccaggca caggcacagg agcgtcattc
      421 tgacagcaac cccgtattcc gcctgacgga aataggctgc attcggaaga tcgtctacat
      481 ccgttgaaag ctccgaaact ctggggagcg ggtgaagcac agtcgtattt tttcgggatg
      541 cgaggatcag ctccttgttt accttgtatg tctctttgag tttttcgtat tccctgtgat
      601 ccgggaatct ttcttcctga attctggtta catatacaac atcacagtcc caaatggcct
      661 tgatatctct tgtttcttca aacgtcagaa gggaattgga aggatccttt ttataattgt
      721 caggaagaga cagttcttcc ggggaaatca ggtacagatg aatcttgtag tgatgcagaa
      781 gcctgatcag tgaatgaatt gttcttccgt actttagatc acccacaaag ccgacagtga
      841 tattgtccag agtacccttt tcggatttga ttgtgtacat atccagaaga gcctgggtag
      901 gatgctgtcc tgcgccgtcg cctgcattga tcacaggcat ttttaccgct ccggcagcga
      961 tcctggatga tccttctacc ggatgacgga tgactgcgat atcggcatat gcctcaatca
     1021 ttttcattgt gtcatagagt gtttctccct tggagatgga agaaaactga aaacccacag
     1081 tggagatgac acgtcctccc agcctttcca ttgcagattc aaaagaaagc ctggtccttg
     1141 tcgatgcctc aaagaaaaga gaggcaagca gttttccatg gagcagtccg aatgcctgac
     1201 cggaatgaag cagttccttc atcctgttgg tttttgaaat aatgaagtag gttgactgat
     1261 agcaggattt tatgaagatt caaaaggagc attttctgcc tttttaaaaa gattaactgc
     1321 ctaagaactg ggatttgaat tgctcaagaa aggattttta gaatcttttt ctggactttt
     1381 aataaaaggg ttgggctttt gattttcttg atttggtaaa aatggatttg gattaacggt
     1441 ttttacttca gccaatttgt ctttgtcaga ttcaggagat aaaaaga
//
LOCUS       DAOQGJ010001228         1486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65613_length_1486_cov_3.002096, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001228 DAOQGJ010000000
VERSION     DAOQGJ010001228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65613_length_1486_cov_3.002096"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PK683_16655"
     CDS             complement(<1..729)
                     /locus_tag="PK683_16655"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HNH10130.1"
                     /translation="MSFRIDAHCPSCGAVSEIRNPAVQQVTCSYCGTLFLWDKDGVKD
                     LGIKSKLMPAISGLAIGTSGKLSGKSFVVMGRVQYAYAYQSGEEASRWDEWYLDLGNE
                     ESWISEDAGRLYLQVKANLTVPVKEEQVSVGTPLEVEGKSYRVKEVGTALCKGAEGCL
                     PFKITPNESYLNVDALAEDGTNITLEFEDDEPLVFIGRLLTENDIQYKKEVSATSKKS
                     TEALRCKNCGSPIAMAGNPSEIRTV"
     gene            762..1151
                     /locus_tag="PK683_16660"
     CDS             762..1151
                     /locus_tag="PK683_16660"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNH10131.1"
                     /translation="MQVFFWRVRCLSIPALAGTDQGANPVELILASLASCQEIVISAY
                     ANALGISFEKVTVDVKGELDLRGFFNLAEVRPGFDTIRLVTTIHTKETDPEKLAQLSH
                     LAKNNCPVLDIIKNPVPVNETIEFRHS"
BASE COUNT          402 a          343 c          314 g          427 t
ORIGIN      
        1 gactgttctg atctcagaag gatttcctgc catagcaata ggagatccgc agttcttgca
       61 tctgagtgct tcagtcgatt ttttggatgt ggccgaaact tctttcttat actgaatgtc
      121 attttccgtc agaagtcttc caatgaatac taacggttca tcgtcttcaa attccagagt
      181 gatgtttgta ccgtcttctg cgagcgcatc cacattgaga taggattcat ttggagtgat
      241 tttaaacggg agacagcctt ctgctccctt gcaaagcgca gtgcctactt ccttgacccg
      301 atagcttttc ccctccactt cgagaggagt tcctacagag acctgctcct ctttgactgg
      361 aacagtaaga ttggccttta cctgaagata aagcctgccg gcgtcctcgg aaatccaaga
      421 ctcttcattt cctaagtcca gataccattc atcccatctg gaggcttctt ctcccgactg
      481 ataggcatat gcatactgca cccttcccat cacgacaaaa cttttcccgc tcagtttccc
      541 ggaagtgccg atcgcaagcc ctgaaatggc cggcatcagc tttgacttaa tacccagatc
      601 cttgacaccg tctttgtccc aaagaaatag ggttccacaa taactgcagg taacctgctg
      661 tactgccggg tttctgattt cagaaactgc tccgcagctc ggacaatggg catcaatcct
      721 gaatgacata cgggtatcaa tctgcaggtt actggatttg gatgcaagtc tttttttgga
      781 gggtcaggtg cctgtcaata cctgctcttg caggaacaga tcagggagca aatcctgtgg
      841 agctgattct ggcttcgctt gcatcctgcc aggagattgt catttcagcc tatgccaacg
      901 ctctgggaat atcttttgaa aaggtgactg tggatgtgaa aggagaactc gacttgagag
      961 ggtttttcaa tctggcagaa gtaagaccgg gatttgatac aatccgactg gttacaacga
     1021 tccatacgaa agaaacagat cctgaaaagc tggcacagct ttctcatctg gcaaaaaaca
     1081 actgcccggt gctggatatc atcaagaatc cagttcctgt aaatgaaaca attgaattca
     1141 ggcattcctg aatatgcagt gctgttgggg ctgtgcaaaa gcactgtttt tctcacagag
     1201 aaaagatcaa tagaaccagt atcttccggg aaccgttttt tatgctttta cacattcctg
     1261 cccgtcagaa caaaactttt ctctgccaat ttgccgaaaa cctgatttga atatattttt
     1321 tttggataat cccttaaaga ggcaatatcc tgagtctgaa aaaaaaggat attaggaaat
     1381 acttttactg aagaattcct gatcagagaa aaattcagtc taagccttag gctttccaag
     1441 taaaaaaatt ttattagagg tgagacttta tggtcaaagt atttac
//
LOCUS       DAOQGJ010001229         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65755_length_1484_cov_4.589923, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001229 DAOQGJ010000000
VERSION     DAOQGJ010001229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65755_length_1484_cov_4.589923"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..>1484
                     /locus_tag="PK683_16665"
     CDS             54..>1484
                     /locus_tag="PK683_16665"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH10132.1"
                     /translation="MGVSCSNEAVFAPKAVNGVIDLRNVNLDHPVRLGGDWGIHWKEF
                     IPPEKNSDPAELFPVPGVWPKGKEPIRYATYTLKILLPEKYLDNAEFLFYDQGSNYAA
                     FVNGVFLGKDGEPSKAKSDIADYWPKVFTCRDRSHTLNLTVHISNHHHSESGLWYPIY
                     IGSPTGFRNYFFLKAVKAFVLVGSILMIGFYHFIVFMNRRLDITTLYFSYLCIGVALR
                     ILVTEERFITYWFPLRGEQLYTLEYISFYALPPLMAGFIHKIFPEEYSARMKYLMQIL
                     CVLSIISLFLAPMELFTGFIVYFRIFILFGIAHAIYALIMAYVRKREGSAPMLFGNLA
                     LLITGGNDILFISGIISTFYMLPVGIIVFIFSQAFLLSIRYSKSFSDADMHYRNLQKS
                     NEELNLVRLQLEEAVETRTKQLDRTLKSLRDDLNIAKKIQKAIISKIPPNEMGLNFSI
                     RYFPMGDVGGDFYDVTYLRHDYVRIFM"
BASE COUNT          385 a          311 c          334 g          454 t
ORIGIN      
        1 gggagatttt gcgcaggtta ttctattttc tggcttatgc gggactgtta tttttgggcg
       61 tttcgtgttc aaatgaagca gtttttgcac ccaaggcagt caatggagtc atagatctta
      121 gaaatgtaaa tctggatcat cctgtaagac tgggagggga ttggggaatc cactggaaag
      181 aatttattcc gcctgaaaaa aattccgacc ctgctgaatt gtttcctgtt cccggagtct
      241 ggcctaaggg aaaagagcca attcggtacg caacttacac actgaagatc ctccttccgg
      301 aaaaatacct cgacaatgca gaattcctgt tctatgatca gggatcaaat tatgcagcct
      361 ttgtgaacgg agtctttctg ggaaaggacg gagagccttc caaggcgaag tctgatatcg
      421 cggactactg gcctaaggta tttacctgca gagacagatc ccatacactg aatctgacag
      481 ttcatatttc caatcatcat cattccgagt caggactctg gtacccgatt tatataggaa
      541 gtcctacagg gttcagaaat tattttttcc tgaaggcagt gaaggctttt gttcttgttg
      601 gctccattct gatgataggc ttttatcatt ttattgtatt catgaacaga agactggaca
      661 taacgacgct ctatttttcg tatttatgca tcggtgtcgc tcttcgtatt ctcgtgacag
      721 aggagagatt catcacttac tggttccctc ttcggggaga gcagctctat accctggagt
      781 atatcagctt ttatgctctg cctccactaa tggccggatt tattcataag attttcccgg
      841 aagaatattc tgccagaatg aaatatctca tgcagatcct atgtgtgctt tcaatcattt
      901 cactctttct cgcgcctatg gagctcttta ccggctttat tgtctatttc agaatattca
      961 ttctgttcgg gattgcccat gcgatatatg cattaattat ggcgtatgtc aggaaaagag
     1021 aaggttctgc tccaatgctc ttcggcaatc tggctcttct gatcacaggg ggaaatgata
     1081 tactgttcat cagcggaata atctccacat tttatatgct tccggtgggc atcatagttt
     1141 ttattttcag tcaggcattt cttctgtcca tccgatattc caagtcgttt tcggatgcgg
     1201 atatgcacta caggaatctg cagaagtcca atgaggagct gaatctggta agacttcagc
     1261 tcgaagaggc cgtcgaaacc aggacaaagc agctcgacag aacattgaag tctctccggg
     1321 acgatctgaa catagccaag aaaatacaga aagcaatcat cagcaagatt ccgcccaatg
     1381 aaatgggact caatttttcc attcggtatt ttccgatggg ggatgtagga ggggatttct
     1441 atgacgttac gtatctgcgc catgattatg taaggatttt tatg
//
LOCUS       DAOQGJ010001230         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65854_length_1483_cov_3.175770, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001230 DAOQGJ010000000
VERSION     DAOQGJ010001230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65854_length_1483_cov_3.175770"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PK683_16670"
     CDS             <1..397
                     /locus_tag="PK683_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10133.1"
                     /translation="ELLPLDLVESEPLSAAIYDLKKNFNDDDLKECLPVYCIEKAYLP
                     EMEDLTREEVQHFASEVFRLDQSRNSVKQDILFKVENRILHYGLNLIFTLKKEYNSDL
                     YRKIRQKIESVDKDIIERTEANLAARKKI"
     gene            complement(407..>1483)
                     /locus_tag="PK683_16675"
     CDS             complement(407..>1483)
                     /locus_tag="PK683_16675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH10134.1"
                     /translation="NGIIGMTELLLNTELTEKQKKYATTVYSSGEVLLSLINDILDFS
                     KIQSGKMNIHLSECNLKKLTGDLMKTFSMQAEKKNLKMLLNFPENIPGNVRADSVRIR
                     QILFNLMGNALKFTNEGSVSLDVRIFPSQSRGILNYQFTVKDTGIGIPAERVGELFKE
                     FTQIDMSAERKFGGSGLGLSISKNLAELMDGSISYSPREERGSKFTLTLPLEISTDSS
                     DFNESQNHPVRISATDSQKKLKVLAVDDNEMNLLLIRSMLEMMNMDNDEAVSGKDAIE
                     LISQNNYDLILLDIEMPEMSGLETLKEIRKIEIAQSRKIFIIACTAHALEGSREKFMN
                     AGFNAYLSKPISLFRLKETLAMLS"
BASE COUNT          413 a          311 c          294 g          465 t
ORIGIN      
        1 ggagctcctc cctctcgatc ttgtggaatc tgaacctctc tctgctgcga tttacgatct
       61 gaaaaaaaat tttaacgatg atgatctgaa ggaatgtctt ccggtatact gcatagagaa
      121 ggcttatctt cctgaaatgg aagatctgac gagggaagag gtgcagcatt ttgcatctga
      181 agtatttcgt ctggatcaga gcaggaattc agtcaagcag gacattcttt tcaaagtaga
      241 aaaccggata ctgcactatg ggctgaatct gattttcacc ttaaagaaag aatacaactc
      301 ggatctttac cgtaagatca gacagaagat agaatcggta gacaaggaca ttatcgaaag
      361 aactgaggca aatcttgctg cccgaaaaaa aatctaaggc tcatattcag gaaagcattg
      421 cgagagtctc cttcagtcta aaaagtgaaa tcggctttga cagataagca ttgaagcctg
      481 cattcataaa tttctccctg cttccctcca gcgcgtgcgc agtacaggca atgatgaata
      541 tttttctgct ctgagcaatt tctatttttc tgatttcctt aagtgtttcc agtccgctca
      601 tttccggcat ctcaatgtca agtaaaatta aatcataatt attttgagat ataagttcga
      661 ttgcgtcctt tccgctcaca gcctcatcat tgtccatatt catcatttcc agcattgaac
      721 gtatcagaag cagattcatt tcattgtcgt ccactgcaag tactttcagc tttttctgag
      781 aatctgttgc actgattctg acaggatgat tctgactttc gttaaaatca gaactgtccg
      841 tcgatatttc gagagggaga gtcagtgtga atttagagcc tctttcttct ctgggggaat
      901 aggatatgga tccgtccatc agctcggcaa gatttttgct gatagaaaga ccgagaccgc
      961 ttccgccgaa tttcctctct gccgacatat caatctgagt aaattcctta aagagttctc
     1021 cgactctttc tgcaggaatg ccgatgcctg tgtccttgac tgtaaactga taattcagta
     1081 ttcccctgct ttgagaggga aaaattctta catccagaga gactgaacct tcattagtaa
     1141 attttaaggc atttcccatc agattgaaaa gtatctgcct gattctgaca gagtccgcac
     1201 ggacatttcc cggaatgttt tcaggaaaat tcagaagcat tttcagattt ttcttttctg
     1261 cctgcatcga aaaagttttc atcagatctc cggtcagctt tttcagattg cactctgaaa
     1321 gatgtatgtt catttttcct gactgaattt tggaaaaatc cagtatatca ttgatgaggg
     1381 ataataatac ttccccagag ctatacacgg tggttgcata ttttttctgt ttctcggtaa
     1441 gttcggtatt caagagcagt tctgtcattc cgataatacc att
//
LOCUS       DAOQGJ010001231         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65856_length_1483_cov_3.137955, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001231 DAOQGJ010000000
VERSION     DAOQGJ010001231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65856_length_1483_cov_3.137955"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PK683_16680"
     CDS             complement(<1..1277)
                     /locus_tag="PK683_16680"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH10135.1"
                     /translation="MLSLSGYKNLLKIYESSNSDIYEAETDEGRKVIVKVLQELHPNR
                     NQVERFYSEFEIANQIQSDYVRKGISKDRQHGRSILILEFAEGISLKNFFMGTKIDFS
                     LFLKTAASVSDALFHIHKHNVIHKDFNSNNIIIGNNSVKIIDFGISTKISYKSHYLGN
                     PEKLEGTLAYISPEQTGRMNRAVDYRTDIYSFGVSLYEVLSGRLPFFSEDPLELIHLH
                     IASKAKPLSELLLGSERFSAPVPSIFLEAVSEITMKLLEKNAEDRYQSAFGLKNDFQL
                     LYNYFSSKKFPEKFFLQKADFSGRFQIPEKLYGREKESSVLLEAYERIRNSSKELMLV
                     GGYSGVGKSALVHEVHKPNTASRGIFLEGKFDQYQKNIPYSAWIYAFKEFANNLLTES
                     ERSLQNWKEKILSALSGNGKVLTDLIPNLELIIG"
BASE COUNT          430 a          280 c          275 g          498 t
ORIGIN      
        1 ccaatgataa gttccagatt gggaatcaga tctgtcagaa ccttgccgtt tccgcttaag
       61 gcagaaagaa ttttctcttt ccagttttga agagaccttt cgctttcagt caggagattg
      121 tttgcaaatt ccttaaaagc atatatccag gcagaatatg gaatattttt ctgatactga
      181 tcgaatttcc cttccagaaa aataccgcga cttgctgtat tcggcttgtg aacttcatga
      241 acaagtgcag attttcctac tccggaatag cctcctacga gcatcagttc cttggaagaa
      301 tttctgattc tttcataggc ttccagcaga accgaagact ctttttctct tccgtaaagt
      361 ttttcaggta tctgaaatct tcctgaaaaa tcagctttct gcagaaagaa tttctcagga
      421 aattttttag atgaaaaata attataaaga agctgaaaat catttttaag accgaacgcg
      481 gactgatatc tgtcttctgc atttttctcc agaagtttca ttgtgatttc agagacagcc
      541 tcgaggaata tactgggaac aggagcagaa aatctttcag aaccaaggag gagttcagac
      601 agaggtttgg ctttggaagc aatatgaaga tgaatgagtt ccagcgggtc ttctgaaaaa
      661 aatggaagcc ttcctgaaag aacctcataa agacttactc cgaatgaata gatgtccgtt
      721 ctgtagtcta ctgccctgtt cattcttcct gtttgctccg gtgatatata tgccagtgtt
      781 ccttccagct tttccggatt tccgagataa tgagatttat agctgatttt agtggaaatt
      841 ccaaaatcaa tgatctttac agaattattt ccgattataa tgttatttga attaaaatct
      901 ttatgaatga cattatgttt atgaatatga aaaagagcat cggagacgct ggctgctgtt
      961 ttcaggaaca gagagaaatc aatctttgtt cccataaaaa agtttttcag actgatccct
     1021 tctgcgaatt caagaattaa tatgctgcgg ccatgctgtc tgtccttgct tattcctttc
     1081 ctcacatagt cagattgaat ttgattcgca atttcgaact ctgaatagaa ccgttcgacc
     1141 tgattccggt tcggatgaag ttcctgcaga acttttacaa tgactttcct gccctcatct
     1201 gtctctgctt catatatgtc agaattactg ctctcatata tttttagtaa gtttttatat
     1261 cctgacagtg acagcattga tattcccctg aatattatat ttttttcaga ttcactgtat
     1321 gtaaagaaaa tcatttttta ttttatgttt cagatggaaa attgcagtcg ggtgaaagag
     1381 gtattcaacg gcaaaatacc tcggaagttt cgaaaggctc taagcactga ttcgtttaaa
     1441 acggtttact atgctcagat attgaattgt tccttatata tat
//
LOCUS       DAOQGJ010001232         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65881_length_1483_cov_2.439776, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001232 DAOQGJ010000000
VERSION     DAOQGJ010001232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65881_length_1483_cov_2.439776"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1168
                     /locus_tag="PK683_16685"
     CDS             47..1168
                     /locus_tag="PK683_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10136.1"
                     /translation="MNISFAFRISLILLIVSFISFISFIYFEDFTVFAEIQKKTNFSI
                     SEETVVNRLLGALLLSFGISAVLYALLARLILSIRKISLMILDWGRDSLNHTDLYKEL
                     YDKELNELKLIFRSALFSSRERDMKRKNDEMREWKENLLHTIQPNLSGLPEEISARLG
                     AAIEPSGQSSPRSDYANTIYTRDGIVSFIAGFESPSTSQTAYFPRFQSVFSCLLELNY
                     LDIAKISHSFLSGFHPYPIHDLRLCMMEWDRDKRLLRYLHYQKNPVYFLDSFAVRELP
                     VNGETSYQFDGDIPDFRHFVPPVSGFLITFSDRIRTYRGFQDMSFLIPLEKMLVEKYY
                     EFSDPVILVRQIHSFFFEWQKQKGDAESLAPVRILAIQV"
     gene            1219..>1483
                     /locus_tag="PK683_16690"
     CDS             1219..>1483
                     /locus_tag="PK683_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10137.1"
                     /translation="MQEFPEDSKISGWNISSDDPVLENVFRKNFSKNGVVFSGNRIHL
                     NARIEEETQNVFSNILYSILWLTLYFRHDTEASWVYELTLYEKN"
BASE COUNT          400 a          300 c          304 g          479 t
ORIGIN      
        1 cctttcaggc aacttcagga agaattcttg tgcttgctat gaaattatga acatctcctt
       61 tgccttcaga atctcattaa tactgctgat tgtttctttc atttccttca tcagtttcat
      121 ctatttcgag gattttactg tatttgcgga gattcagaag aagacaaatt tcagcatcag
      181 cgaagaaact gtagtcaaca gactccttgg agcccttctg ctttcatttg gaatctcagc
      241 tgtcctttac gcacttcttg ccagactcat tctttcgatt cggaaaattt cactgatgat
      301 actcgactgg ggccgggatt ccctgaatca tacggatctt tataaagagt tatatgataa
      361 agagctcaac gaactgaagc tgatattccg ctctgcactt ttttcttcca gagaaaggga
      421 tatgaaaaga aaaaatgatg agatgagaga atggaaggaa aacctgcttc atacaattca
      481 gccgaatctt tcaggactgc ctgaggaaat ttcagcaagg cttggggctg caatagagcc
      541 tagcggacag tcttctcccc gaagcgatta tgccaataca atatatacca gggacggaat
      601 tgtgagtttt attgcgggct tcgaatcgcc ttccacttca cagactgctt attttccgag
      661 gtttcagtct gttttcagct gtcttcttga actgaattat ctggatatag caaaaatatc
      721 tcattccttt ctttcaggat ttcatcctta tcctattcat gatctcagac tctgtatgat
      781 ggaatgggac agagacaaaa ggcttcttcg ctacctgcac tatcagaaaa atcctgtcta
      841 ttttctggat tcatttgctg tcagagaact gcctgtcaac ggtgaaacca gttatcagtt
      901 tgacggggac attcctgact ttcgtcattt tgtcccgcct gtcagcggct ttctaattac
      961 tttctcggac aggatcagga cttacagagg ctttcaggat atgagttttc tgattcctct
     1021 ggaaaagatg cttgtggaga aatactatga attttccgat cctgtaattc tcgtaagaca
     1081 gatacattca tttttttttg agtggcagaa gcagaaggga gatgctgagt ctctggcccc
     1141 ggtcaggatt cttgcaatac aggtgtgaga atcctctgtc ttatttttct tcttcagttc
     1201 tgcagttcag ttccaaagat gcaggagttt ccggaggatt caaaaatatc gggatggaat
     1261 atcagttcag atgatccagt tcttgaaaat gtgtttcgga aaaatttttc aaagaacgga
     1321 gttgtctttt ccggtaacag aattcatctc aatgcaagga tagaagaaga aactcagaat
     1381 gttttttcca atatattgta ttccattctc tggcttactc tttattttcg gcatgatact
     1441 gaggcatcct gggtatatga actgactctg tacgaaaaaa atg
//
LOCUS       DAOQGJ010001233         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65884_length_1483_cov_2.331232, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001233 DAOQGJ010000000
VERSION     DAOQGJ010001233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65884_length_1483_cov_2.331232"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK683_16695"
     CDS             complement(<1..366)
                     /locus_tag="PK683_16695"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNH10138.1"
                     /translation="MKIICVGSHPDDVELAMGGTAAFLSHSGHEILFADLSDGEPTPR
                     GDRETRILESSESAEILKVKRILLNNRNRYFEDTVSARESLAEVFREFRPGLIFTHFP
                     YDAHPDHISACRITDAARFY"
     gene            complement(363..842)
                     /locus_tag="PK683_16700"
     CDS             complement(363..842)
                     /locus_tag="PK683_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNH10139.1"
                     /translation="MAGKQKAYWLFKSEPDVFSIQDLAESRNQTASWEGIRNYQARNY
                     LRDQVKPGDDVLFYHSRIEPIGVAGIATVVKGGYVDHHAFDSKSDYYDSKSRPEKPQW
                     YMVDVRLKLIFNHVVTLKKIKTYPELQNMALIRSSRLSIQPVTEDEFKFILEIGRNS"
     gene            complement(944..>1483)
                     /locus_tag="PK683_16705"
     CDS             complement(944..>1483)
                     /locus_tag="PK683_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH10140.1"
                     /translation="VLFGGAVAVLPIFASDILRVGPDGLGMLRAAPAVGAALMAVFLA
                     YHPPKKNAGRALLFAVAGFGVTIVLFGISQNFLLSIFFLGLSGAFDCISVVLRSTIMQ
                     TFTPDEMRGRVSSVNSIFIGSSNELGAFESGVAAKFLGTVTSVIFGGCMTIAIAAGVA
                     LRSPVLRRLDFTRSETVSK"
BASE COUNT          419 a          338 c          321 g          405 t
ORIGIN      
        1 atagaatctg gctgcatcag taatccggca ggcagaaata tggtccggat gagcatcgta
       61 aggaaaatga gtgaaaataa gaccgggtct gaattccctg aatacctcgg caagggattc
      121 tcttgcagaa acagtgtcct caaaatatct gttcctgtta ttcaggagaa ttctttttac
      181 cttcagtatt tctgcagatt cagacgattc aagtattctg gtttctctgt cccctctggg
      241 agtcggctct ccgtcggaaa gatctgcaaa aagaatctca tgacctgaat gagaaagaaa
      301 cgctgctgtt cctcccatcg caagttcaac atcatccgga tgggagccca cacagatgat
      361 tttcatgaat tcctgccgat ctccagtatg aacttgaatt catcttcagt cacaggctga
      421 atggaaagac gggaactcct gataagtgcc atattctgca attcgggata tgtcttaatt
      481 ttttttaatg tcactacatg attaaatatc agcttaagcc tgacatcgac catgtaccac
      541 tgaggctttt ccggtctgct ctttgaatca taataatcgc ttttagaatc aaatgcatga
      601 tgatccacat atccgccctt gactacagtg gcaatgcctg caacgccgat cggttcaatg
      661 cggctgtgat aaaaaagtac atcatctccc ggcttgacct gatctctgag ataatttctt
      721 gcctgatagt ttctgatccc ttcccaggat gctgtctgat tcctggactc tgccagatcc
      781 tgaatggaaa atacatctgg ttctgatttg aaaagccagt acgctttctg tttgccggcc
      841 ataggatttt cagtcttttg agatgaagtt tttttacaag taatttactg atcttttcga
      901 aaaatgaaac tgtatttgaa ttgaatgctc ttcatgcata aatctatttt gaaacagttt
      961 ctgaacgggt gaaatcaagc cgtctgagca cgggtgagcg cagagcgaca cctgcagcaa
     1021 ttgcgatagt catacagccg ccaaatataa ctgaggtgac tgttcccaga aatttagctg
     1081 caactccgga ttcaaaggcg ccaagctcgt ttgatgatcc tatgaatatg ctgttcacag
     1141 aagatactct tcctctcatt tcatccggtg taaatgtctg cataattgtg gagcggagaa
     1201 ctacactgat gcagtcgaat gcaccggaga gtcctaggaa gaaaatggaa agcaggaagt
     1261 tttgtgaaat cccgaagagc acaatcgtta ctccgaatcc tgcaacggca aaaagaagtg
     1321 ccctgcctgc atttttcttg gggggatgat aagcgagaaa tacagccata agagctgctc
     1381 ctacagcagg ggccgctcga agcattccca gaccgtccgg acctaccctc agaatatcag
     1441 aagcgaaaat aggcagcact gcaactgctc ctccgaaaag aac
//
LOCUS       DAOQGJ010001234         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65947_length_1482_cov_2.857744, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001234 DAOQGJ010000000
VERSION     DAOQGJ010001234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65947_length_1482_cov_2.857744"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK683_16710"
     CDS             <1..572
                     /locus_tag="PK683_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10141.1"
                     /translation="GGSDPGGGGVASGGFDPAHSVMYMFYVANTGSFTVTVTNAPYSV
                     KLPAANTLIKIYNAGGTQVGSSTGTGTASVSIGSAGWYFAEIAGTSRSAYGFFDVKVT
                     GPTQTSAGTLGSWTTNNLILSWYDHTNRIIHIAGSGESGSVGNINITAMAEYGKIIRG
                     DFTATLRQVTAAGTTTAVTAGYFNLIRAE"
     gene            582..1208
                     /locus_tag="PK683_16715"
     CDS             582..1208
                     /locus_tag="PK683_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10142.1"
                     /translation="MKRIIMIIALFASGLHSAPRTMKLNSIMFQVGEKNFYFEDTVYC
                     SLETRNGTSEIVVALFDEAARVKLTITARVPAGEEKEEMRLSTNFHSIAAVLSSPHGG
                     FMIYPAVSLQPSAEIMSYSKSHIGQPVSPEWRGMTRSERIAKGKGVSIDRGMEGTSFY
                     LNLIPIFDENNEIREFAGSFSGTVRHVGKNQGRMQPVSGGEFRIGVMK"
     gene            1205..>1482
                     /locus_tag="PK683_16720"
     CDS             1205..>1482
                     /locus_tag="PK683_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10143.1"
                     /translation="MKLFIKFFIFVSAVGGLLSFVDNAHQNLPEDALLYIESYGSNQQ
                     RWAFDYLKAKAGGRYMDSSSNAGDYSATVGGPGQNSAGPKFGDVYGNGG"
BASE COUNT          438 a          313 c          379 g          352 t
ORIGIN      
        1 gcgggggatc tgatccgggc gggggcggtg ttgcatcagg cgggttcgat ccggcacaca
       61 gtgtaatgta catgttctat gtggcaaata cgggaagttt tactgttaca gtcacgaatg
      121 ccccgtattc cgtgaaactt cctgcagcaa acactctgat aaaaatttac aatgcaggag
      181 gaacacaggt tggctcgtcc accggaacgg gaactgcttc tgtttccatc ggatcagcag
      241 gctggtactt tgctgaaatt gcaggaacat ccagaagcgc ctacgggttt tttgatgtaa
      301 aggtgactgg tccaactcag acatccgcag gaacactggg gagctggact acaaacaatc
      361 tgattctttc ctggtatgat catacaaaca gaatcatcca tatagcagga agcggagaat
      421 ccggttctgt cggaaacatc aatatcactg ccatggcaga atatgggaaa atcatcagag
      481 gcgatttcac tgccacattg aggcaggtaa ctgctgcagg gaccacaacg gcagtgacgg
      541 ccggctactt caatctgatt cgggcagaat aggagaataa gatgaaaaga attatcatga
      601 taattgcatt atttgcgtca ggtcttcatt ctgcaccccg cacaatgaag ctgaacagca
      661 ttatgtttca ggtgggagaa aaaaatttct actttgaaga cactgtatac tgctcattgg
      721 aaactagaaa cgggacgagc gaaattgtag tggctctttt tgatgaggca gccagagtaa
      781 aactgaccat cactgcaagg gttccggccg gagaagaaaa ggaagaaatg agactttcca
      841 ccaattttca cagtattgca gcggtcttaa gttccccgca cggcggattt atgatatatc
      901 ctgcggtatc acttcagcct tctgccgaaa tcatgtccta ttccaaatcc catatcggac
      961 agcccgtctc tcctgaatgg agaggaatga caagatcgga aagaatagca aaagggaaag
     1021 gcgtttccat tgacagggga atggaaggca catcctttta tctgaacctg attcccatat
     1081 ttgatgaaaa caatgaaatc agagaattcg caggaagttt ttcaggcaca gtcagacatg
     1141 taggaaaaaa tcagggaaga atgcagccgg tcagcggcgg tgaattcaga ataggagtaa
     1201 tgaaatgaaa ttattcataa aattctttat atttgtttcg gctgtcggag gacttctgtc
     1261 ctttgtggat aatgcacatc agaacctccc cgaagatgcc cttctttata tcgaatcata
     1321 cggatcgaat cagcagagat gggcatttga ctacctgaag gcaaaggcag gaggaagata
     1381 tatggattct tccagcaatg caggggacta cagtgccaca gtcgggggcc ccgggcagaa
     1441 cagtgccggc cctaaattcg gggatgtata tggaaacggc gg
//
LOCUS       DAOQGJ010001235         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_65953_length_1482_cov_2.632796, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001235 DAOQGJ010000000
VERSION     DAOQGJ010001235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65953_length_1482_cov_2.632796"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PK683_16725"
     CDS             complement(<1..904)
                     /locus_tag="PK683_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10144.1"
                     /translation="MLRKIIAFLLVFLFLFSDADAKSRKKKNVKHNSSSKHHSKFKSH
                     KKKTKKGKSSKKKRKKKSGKNLNIVKESSAKLNTDGQGYVYDYEKQIKAVLKSAESAS
                     DPEDKAELYSQAAVSYQYTYKPELYSKAVSYAARAISLTSDNEKKARYYSILGSIYTV
                     MRPADTATAKKSYLKAIELTNDRASKAQYYSLLAAVYQYKEIPAYSEVEKYNVMAAEI
                     SESSAERANYYAAAAGIYYSRNREKSLKYYTLALNSVQDAETKSYYYSTIAEMYMNSY
                     GEMNEKDLNTGISYMQKAVDTSSSP"
     gene            complement(914..1396)
                     /locus_tag="PK683_16730"
     CDS             complement(914..1396)
                     /locus_tag="PK683_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH10145.1"
                     /translation="MLITQNKVVTIDYTLKDEDGEVIDSSVGSEPLMFLFGAGNIISG
                     LEKALEGKKEGEAVQVSVKPEEGYGLYRDELVQEVALDMFESGVTVVPGMQFQAEQPE
                     GVVIFTVLSVGDKSAVLDGNHPLAGSVLNFDVKVLGIRDATKTELDHGHVHAPGHHHH
                     "
BASE COUNT          385 a          332 c          273 g          492 t
ORIGIN      
        1 caggagacga ggatgtatcc acagctttct gcatataact gattcctgtg ttcagatcct
       61 tctcattcat ttcaccgtaa gaattcatgt acatttcagc aatagtcgaa taataatacg
      121 actttgtttc tgcatcctga accgaattca gggcaagagt gtaatatttc aggctctttt
      181 ctctgttcct gctgtaataa attcctgcag cggctgcata gtagtttgcc ctttctgctg
      241 acgattcaga aatttcagca gccattacat tgtatttttc cacttctgaa tatgcaggaa
      301 tttccttgta ttggtaaact gcagcaagaa gtgaatagta ctgcgccttg cttgctctgt
      361 cattcgtaag ttcaatcgcc ttcagatagc tctttttggc tgttgcagta tctgcgggcc
      421 gcatgactgt ataaatcgaa ccgagaatgg aatagtatct tgcttttttt tcattgtctg
      481 aagtcagaga aatagccctg gcagcatacg acactgcctt ggaatagagc tcaggtttat
      541 aagtatactg ataacttaca gctgcctgag aataaagctc cgccttatcc tcaggatcag
      601 aagcagattc tgcagactta agcaccgctt tgatctgttt ttcgtaatcg tacacatatc
      661 cctgaccgtc agtattcagc tttgcagaag attccttcac aatgttcaga ttctttccgg
      721 attttttctt ccttttcttt ttggaagatt ttcctttctt ggtttttttc ttatgagatt
      781 tgaattttga atgatgcttt gaagaagaat tatgctttac attttttttc tttctggatt
      841 ttgcatcagc atcgctgaac aggaaaagga aaacaagtaa aaatgcgatt atttttctca
      901 gcataatgat tccttaatga tgatggtgtc ccggtgcatg aacatgtcca tgatccagct
      961 cagtttttgt tgcatctctg atccccagaa cttttacatc aaagttcagg actgatccgg
     1021 caaggggatg gttcccgtcc agaacagcag atttgtcacc tactgaaaga actgtaaaaa
     1081 tgactacacc ttccggctgt tctgcctgaa actgcatccc tggaaccact gtgactcccg
     1141 attcaaacat atccagcgca acttcctgaa cgagttcgtc tctgtaaaga ccgtaacctt
     1201 cttccggctt gaccgaaacc tgtacagcct caccttcttt tttgccttcc agtgcctttt
     1261 ccagacctga aataatattt cctgctccga aaagaaacat cagcggttct gaaccgacag
     1321 acgaatcaat gacttctccg tcctcatcct tcagagtata atctatcgta acaactttgt
     1381 tctgtgtaat caacatacgt tcagaattat tttactcgaa ttttacacaa gtgaaattaa
     1441 gatgtttttt cgaggaaggg gattccctgc tcaatatttt tc
//
LOCUS       DAOQGJ010001236         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66094_length_1480_cov_2.935439, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001236 DAOQGJ010000000
VERSION     DAOQGJ010001236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66094_length_1480_cov_2.935439"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PK683_16735"
     CDS             <1..1146
                     /locus_tag="PK683_16735"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNH10146.1"
                     /translation="VSGFGGRVIRPSEEGKYINSQASLVYDKSRMFYGLYQAQDSIRR
                     IRTAILVEGYLDVIGLHNKEFTHTVAPLGTAFTVFQLKLVKNIADELILLFDGDSAGR
                     KAAVKAAEICIREGMPGRVVLLENGVDPFDLSRTKNTGEIGEILSKSTGHLDFFLSEL
                     LLHAVRESGTELKKKAVDNLFPFLKSLEKETDRELILTEASKKIGISSETLWNDFKKG
                     NPVSFAPEKVDIKTEKKAVSSVQSDLERRFIAKLLKRPEFWEYAEEMQNLRLYDNESA
                     FIWEVMISRYSSGDDTGVNLLLDMPEAIQNIFTGILVDEESLDLEEERKVFRGIIERQ
                     KLFMIEKELDEISGSGSGISEMDRMIRLHSLSGEKQRILEEIRKVES"
     gene            1163..>1480
                     /locus_tag="PK683_16740"
     CDS             1163..>1480
                     /locus_tag="PK683_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNH10147.1"
                     /translation="MENLQSLPEVQKIIAIGKANSEVSYDEINDILPDKILNSEKIDD
                     VFTLLHEMGIDVVEEYSKKFDEPATPLNKEEKEKPGKKRKESQSLSASSEDPIRLYLK
                     EIGK"
BASE COUNT          491 a          243 c          367 g          379 t
ORIGIN      
        1 gtttccggtt ttgggggcag agtaatccgt ccaagcgagg aaggaaaata catcaacagt
       61 caagcctcac ttgtatatga taaaagccgt atgttctacg ggctgtacca ggcgcaggac
      121 tccatacgga gaatcaggac tgcaattttg gtagaaggct acctggatgt gatcggactg
      181 cacaataagg aatttactca tacggttgct cctttaggta cagcgttcac tgtatttcaa
      241 ctgaagcttg tcaaaaatat tgccgatgaa ctcatccttc tttttgacgg agacagtgca
      301 ggcagaaagg ccgccgtcaa ggccgctgaa atctgcatcc gggaaggaat gccgggaaga
      361 gttgtgctgc ttgaaaacgg agtggatccc tttgatcttt ccagaacaaa aaataccggt
      421 gagattgggg agattctgtc taagagtaca ggtcatctgg attttttcct ttctgaactt
      481 cttcttcatg cagtcagaga gtccgggact gagctgaaga aaaaagcagt agataatctt
      541 tttccgtttc tgaaaagtct ggagaaagaa acagacagag aactgattct gactgaagcg
      601 tcaaaaaaaa ttggaatcag ttcagaaaca ttgtggaatg attttaaaaa agggaatcca
      661 gtcagttttg cacctgaaaa ggtcgatatt aaaactgaaa agaaagctgt cagttcggtt
      721 cagtctgatt tagagagaag attcatcgcg aagcttctga aaaggccgga attttgggag
      781 tatgcagaag agatgcagaa tcttcgtctg tatgacaatg aatctgcatt tatatgggaa
      841 gtaatgattt caagatattc ttcaggagac gatacaggcg tgaatcttct tctggatatg
      901 ccggaggcaa tacagaatat ttttaccggc atacttgtgg atgaggaatc cttggatttg
      961 gaagaagaga gaaaagtttt caggggaatc atagaaaggc agaaactatt tatgattgaa
     1021 aaagaactcg atgagatttc aggctcagga agcggaatct ctgagatgga cagaatgatt
     1081 cgactgcata gtctgtctgg ggaaaaacaa agaattctag aagaaattcg aaaggtcgaa
     1141 agttaaaatg agcgaggtgg caatggaaaa tctgcagagt cttccggagg ttcaaaagat
     1201 tattgcaatc ggaaaggcaa acagtgaggt ttcttatgat gaaatcaatg atatcctgcc
     1261 tgataaaatt ctgaattcag agaagatcga tgatgtattc actcttcttc atgaaatggg
     1321 aattgatgta gtagaagagt attctaagaa atttgatgaa ccagccactc ctctaaataa
     1381 ggaagaaaaa gagaagcccg gaaagaaaag aaaagaatct caaagcctga gtgcatcttc
     1441 ggaagatcct atcagacttt atctgaaaga gattggaaag
//
LOCUS       DAOQGJ010001237         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66180_length_1479_cov_2.758427, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001237 DAOQGJ010000000
VERSION     DAOQGJ010001237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66180_length_1479_cov_2.758427"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..698
                     /gene="nth"
                     /locus_tag="PK683_16745"
     CDS             36..698
                     /gene="nth"
                     /locus_tag="PK683_16745"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNH10148.1"
                     /translation="MLPAQRKQKKNKTVEPNQIFELLRTEFGEVRCPLNYSQPHELVI
                     AVILSAQCTDERVNLVTPELFRKFPTLKSFAEADPPSIEKLIFSTGFYRNKAKSISGF
                     AKKLQNEYAGTLPNTIEELIPFPGIGRKTANVILNELFSISEGIVVDTHVKRISKILN
                     LTENTDPVKIEKDLMKKFLPEQWMDISLYFIFLGRKYCTARIRNCAECPLKEVCPSSS
                     NI"
     gene            903..>1479
                     /locus_tag="PK683_16750"
     CDS             903..>1479
                     /locus_tag="PK683_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH10149.1"
                     /translation="MMIELHNITKQFGSFSALENVNFSIPEGELVSLLGPSGSGKTTL
                     LRIIAGLETMDSGEIRFATGITSSTPVQDRNIGFVFQHYALFRHMTVAENIGFGLKVR
                     GAGKKEISIRVEELLELIQLKGLAKRYPSQLSGGQKQRVALARALAAKPKVLLLDEPF
                     GALDANVRKDLRKWLRNLHKEIHVTSIMVTHD"
BASE COUNT          457 a          310 c          327 g          385 t
ORIGIN      
        1 ttctgcaatc gctcctaaaa aaagagtcgg tcgcaatttt gccagcgcaa agaaagcaga
       61 aaaaaaataa gactgtagaa ccaaatcaga tatttgagct tctccgaact gagtttggag
      121 aggtcagatg tcctttaaat tactcccagc cccacgaact agtcattgca gtcatcttaa
      181 gtgcacaatg cacagatgaa agagtcaatt tagtcacgcc agaactgttt cgtaaattcc
      241 ctacactgaa atcgtttgca gaagcggacc caccttccat agaaaaatta atattttcca
      301 cgggctttta caggaataaa gccaagtcta tttcgggctt tgccaaaaaa ctgcagaacg
      361 aatatgcagg aactcttcca aatacaatag aggaactcat tccttttcct gggatcggac
      421 gtaaaacggc aaatgtcatt ctgaatgaac tgttttctat ttcagaagga atcgttgtag
      481 atactcacgt gaaacggatt tccaagattc tgaacctcac ggaaaacaca gatcctgtaa
      541 aaattgaaaa agatctgatg aaaaagtttc tgcctgaaca atggatggac atttcacttt
      601 attttatctt cctcggtcga aaatactgca ctgcacggat acggaactgt gcggaatgtc
      661 ctttgaaaga ggtttgcccg tcatcgtcca atatataaga tctaactgcc aatataacag
      721 atttcttgac tgaaaatcag tctgatatgt tgtggttatt agtatgaatt cagtttaaga
      781 gtttttacag cagggccgaa aggcggtcgg atcacggtgc ttgccgcctg cagaaggctg
      841 caagccttga aagaagcagc gcagtccaaa agtttcaagg gtctgcatag ggattattta
      901 tcattatgat agaattacat aatatcacaa aacagtttgg aagcttcagc gcgctggaga
      961 atgtgaattt ttccattccg gaaggagagc ttgtttctct tctcggaccg tccggttcag
     1021 ggaaaacgac tctgctcaga atcattgcag gccttgagac aatggattcc ggcgaaatca
     1081 gatttgctac aggaatcaca agcagcactc ctgttcagga cagaaacatc ggatttgtat
     1141 ttcagcacta tgctctgttc agacatatga ctgttgctga aaatatagga tttggactga
     1201 aagtaagggg tgcagggaaa aaggaaatca gcatccgcgt ggaagaactt ctggaactga
     1261 tccagctaaa gggacttgcg aaacgttatc cttcacagct ttcgggggga cagaaacaga
     1321 gagtggcact tgcccgtgct cttgctgcaa aaccgaaagt gcttcttctg gacgaaccgt
     1381 ttggagccct cgatgcaaat gtaagaaagg atctccgtaa atggctccgc aatctgcaca
     1441 aggaaattca cgttacctcc atcatggtga ctcatgatc
//
LOCUS       DAOQGJ010001238         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66249_length_1478_cov_3.154603, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001238 DAOQGJ010000000
VERSION     DAOQGJ010001238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66249_length_1478_cov_3.154603"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PK683_16755"
     CDS             <1..669
                     /locus_tag="PK683_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10150.1"
                     /translation="NAFSADRKTKDRRGICLTLSLFYMLFFVFLNLNCRNQEEILLER
                     LNLPGSSDFRKLYSAEKQSSSGKETVHIFQGKNEEHLVVSRTAEDGPHPVFVKTFLHS
                     FYGPYSYSQEKGKWIPDSGKECPCILIQTELKKIGEDSYESLFLKVLSEEPPFGLSAV
                     PFVIRENKVILNGLDMIPEDSKIREEGDYLLELEKDGNILIRSQKNTSKLMLGFKNGT
                     YVRK"
     gene            656..1297
                     /locus_tag="PK683_16760"
     CDS             656..1297
                     /locus_tag="PK683_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10151.1"
                     /translation="MSENNSLSFPAHKEIVRAQIERFHVYYIDYFKREETLRMVEYFF
                     EKVYNVEGKQDWVKIAIEGFDKVKSMIKESTRDSMEELLELNYLTDRLDTMMAELLLK
                     KGWKGEQISREFYDSLFSELGHEEDRKKQLFHVLKNMRQFYSLAHRPINSIILKPAKF
                     MSKILGIYPLFATVEEGYHACLPVKTEIFESFYADVEKKEWNYLKERFPNIIR"
     gene            1294..>1478
                     /locus_tag="PK683_16765"
     CDS             1294..>1478
                     /locus_tag="PK683_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNH10152.1"
                     /translation="MTRERRPARQRKKFAEKDISERWLLTYSDMITLLLGLFIIMYSI
                     SNVDNAKLQAVAGIIRGG"
BASE COUNT          473 a          294 c          343 g          368 t
ORIGIN      
        1 aatgcatttt ccgcagaccg aaagacaaag gacaggagag gcatatgcct gacactgagt
       61 cttttctata tgctgttttt cgttttcctg aatctgaact gcaggaatca ggaagagatt
      121 cttctggagc gtctgaatct ccctggttct tctgatttcc gcaagctgta ttcggcggaa
      181 aaacagtctt catccggaaa ggaaacggtt catatctttc aaggaaaaaa tgaagagcat
      241 ctggtcgtct caaggactgc ggaggacggt ccccatccag tttttgtgaa aacgtttctc
      301 cacagcttct acggacccta ttcttacagt caggaaaagg gaaaatggat tcctgattcc
      361 ggaaaggaat gtccctgcat actgatacag acggagctga aaaaaatcgg cgaggacagt
      421 tatgaaagcc tttttctgaa agttctgtct gaagagcctc ctttcggact gtctgcagtg
      481 cctttcgtca tcagggagaa caaggtcata ctgaacggtc tggatatgat ccctgaagat
      541 tccaaaatca gagaggaagg agattatttg cttgagctcg aaaaggacgg aaatatactc
      601 atccggagtc aaaaaaatac ttcaaaactg atgcttggat ttaagaatgg aacttatgtc
      661 agaaaataac agtctgagtt tccctgctca caaggaaata gtcagagccc agatagaaag
      721 gtttcatgtg tactatatcg actatttcaa aagagaggaa actctccgga tggtggaata
      781 cttttttgaa aaggtataca atgtagaagg caagcaggac tgggtgaaaa tcgcaatcga
      841 gggcttcgac aaggtcaaat caatgatcaa ggaaagcaca agagacagta tggaagaact
      901 gctcgaactc aattatctga cagacagact ggatacaatg atggccgaac tccttctgaa
      961 aaagggctgg aagggagaac agatttccag agaattttat gactctctgt tttccgaact
     1021 cggtcacgaa gaagacagga aaaaacagct ctttcatgtt ctgaagaata tgagacagtt
     1081 ctacagcctg gcacacagac cgatcaattc catcatactg aaaccggcaa aattcatgtc
     1141 aaagattctg ggcatctatc ctctgtttgc cactgtagaa gaaggatacc atgcctgtct
     1201 tccagtgaag actgaaatat tcgaatcctt ttatgcggat gtggaaaaga aggaatggaa
     1261 ctatcttaag gaaaggttcc cgaatataat cagatgacca gagaaagaag accggcaaga
     1321 caaaggaaaa aatttgcaga aaaggacatt tcggaaagat ggctgctgac ctattccgat
     1381 atgatcaccc tccttctcgg ccttttcatt ataatgtatt ccatttccaa tgtggacaat
     1441 gcaaaacttc aggctgtggc aggaatcatt cggggcgg
//
LOCUS       DAOQGJ010001239         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66486_length_1475_cov_2.885915, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001239 DAOQGJ010000000
VERSION     DAOQGJ010001239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66486_length_1475_cov_2.885915"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(599..1030)
                     /locus_tag="PK683_16770"
     CDS             complement(599..1030)
                     /locus_tag="PK683_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10153.1"
                     /translation="MNFMNSSESVKILKSGFFYFLGIFALGFVLGTVRISVLVPRIGA
                     LYGVLLEIPIMLSVSWFFSRWLILRYSVQSGVKPLLAFGFWAFFLLISTETVFALTVF
                     GQTPAEYLAGLQTLPGALGLGAQIVFGIIPALHREQGFKPE"
     gene            complement(1079..>1475)
                     /locus_tag="PK683_16775"
     CDS             complement(1079..>1475)
                     /locus_tag="PK683_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10154.1"
                     /translation="SSRIESLTKYYKCSILISQSTYDELEKESSAGTFAAREADNVTV
                     KGRSKSIRIYEIMVFNSEEERKRMLSLRDDFEKGLLLYKNLQFRQALDAFSSIHNDFL
                     STLYAERCRNFIKNPPAPDWDGVYLSETK"
BASE COUNT          431 a          334 c          299 g          411 t
ORIGIN      
        1 gcaaggcgtt gagcacatca cagaagaact tatcagcaaa ctagcaaaca ttctgaatta
       61 atcttagatg ccgcaaggcg ttgagcaccc tcaaagccac gctccctcat gtattcctgt
      121 ctcgtagctg aattaatctt agatgccgca aggcgttgag cacggaagaa ccgtttcgcc
      181 ttacagcgag ttaatgaact gaatgaatct tagatgccgt taggcgtttt catcaataag
      241 tttttcatta caaatcattt atgcattgta agtcaatgaa cctagcccac ccacccctct
      301 cagatttccc atttagccga tcagacgaca aaacagctct ttcggctgag aaggggcaca
      361 taaaaaaaca ggagggacgc agtttacaaa ataaccgaca agtgcagttt ctgtttcatt
      421 tctccagaat aaaatccgtc atatattaaa ttagaatgta tcagtttttc atgaaagagt
      481 attttttcat tacgcctcag tatctctatg actgcaccct gcatccgtat gagttcatat
      541 ttttcaataa aggatttcag cctgctgttc ctttttctat tctgtattca gaaggaattc
      601 attcaggttt aaaaccctgt tcccggtgaa gcgctgggat gattccgaat acgatctgag
      661 caccaagtcc gagtgcaccg ggcagagtct gaagacctgc aaggtactct gcaggggtct
      721 gtccgaaaac ggtgagtgca aaaactgtct ccgtactgat gaggagaaaa aaagcccaga
      781 agccgaaagc aaggagaggc tttactccgc tctgaacgga ataacggagg atcagccagc
      841 gggaaaaaaa ccaggaaaca gaaagcataa tcgggatctc cagcagaact ccgtacaatg
      901 ctccgatgcg gggcacaagg actgaaatac gcacagttcc aagcacaaaa cccagggcga
      961 agattcccag aaaataaaag aagcctgatt tgagtatttt tactgattcg gacgaattca
     1021 taaaattcat tattttaccg tttcatataa agtccagtca ttctcattcc tttctgcctt
     1081 attttgtttc ggagagatag actccgtccc agtcaggggc gggaggattt ttgatgaaat
     1141 tcctgcaccg ttctgcatac agtgtgctca gaaaatcatt atgaatggag ctgaatgcat
     1201 cgagggcctg cctgaactgt aaatttttat ataataatag tcctttttca aaatcgtccc
     1261 ggagactgag cattctcttc ctttcttctt cagaattgaa taccataatc tcgtaaatcc
     1321 tgatgctttt tgatctgccc ttgacagtca cattgtctgc ttctcttgcc gcgaatgtcc
     1381 cggcactgct ttccttctca agttcatcgt atgttgactg agaaatcagt attgaacact
     1441 tataatattt cgtcagactt tcaatccttg aagag
//
LOCUS       DAOQGJ010001240         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66554_length_1474_cov_3.377026, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001240 DAOQGJ010000000
VERSION     DAOQGJ010001240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66554_length_1474_cov_3.377026"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PK683_16780"
     CDS             complement(<1..259)
                     /locus_tag="PK683_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016760495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10155.1"
                     /translation="MPQRKDISSVLIIGSGPIVIGQACEFDYSGTQATKALKEKGLRV
                     ILVNSNPATIMTDPMLADATYIEPLTVSVLTKIIEKEKPDAV"
     gene            complement(389..850)
                     /locus_tag="PK683_16785"
     CDS             complement(389..850)
                     /locus_tag="PK683_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10156.1"
                     /translation="MIDMNTLETYEKLKEMQFSEEQSKGLARLFGKYSELEPASKRDL
                     SETELRLQKEIEGIRKETEDIRLEIQDVRLEIKNVEMKLYKEIEGTRLEIQDVRLEIK
                     NAEIKLSKDIRELDVKLSVQMESIRSDLIRWFVGASIALGGLLGALIKFGK"
     gene            989..1438
                     /locus_tag="PK683_16790"
     CDS             989..1438
                     /locus_tag="PK683_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10157.1"
                     /translation="MNRTADLFWELIHSGRPKEMNILLDQISISVKDFGSEDWIQLDE
                     AVRKSSPYYASLNMREKNPAEFVPENSTLEKQVLLLGLCSMHPVGFFREKALNLLGKV
                     RDGSELPFLLLRLKDWAGPVRSAAEKMLRERLSSEYSSYYIQFYHLL"
BASE COUNT          401 a          329 c          296 g          448 t
ORIGIN      
        1 gtactgcatc cggtttttct ttttctatga ttttggttaa tacagataca gtcaaaggct
       61 cgatataagt ggcatcggcg agcataggat ctgtcatgat cgtggccgga ttggagttta
      121 caaggatgac cctcaggcct ttttctttca gagcttttgt ggcttgtgtt ccggaatagt
      181 caaattcgca ggcttgcccg atgacgatgg gccccgagcc tatgataagg acggaagaaa
      241 tgtcttttct ttgaggcata atgcaagttt ttttactgca gcccttctaa caatcatttc
      301 tctgatgaaa aaaaagaaga aaatttacat taatagaagg gactaggaaa accgacaagt
      361 catccaaccc ctggatcgtg aaagtctcct atttcccaaa cttgatcaag gcccccaaaa
      421 gtccacccaa tgcaatggat gcgcctacaa accagcgaat caggtcagaa cggatactct
      481 ccatctgcac gctcagtttt acatccaatt cccgaatgtc ttttgacagt tttatttccg
      541 cattcttgat ctccaatcgg acatcctgta tttccagtcg agttccctcg atctccttat
      601 aaagcttcat ttccacgttt ttgatttcca gacggacatc ctgaatctcc aggcgaatat
      661 cttcagtttc cttacgaatc ccttcaatct ctttctgaag acggagttca gtctcgctca
      721 ggtctctttt gctggcaggc tccagctctg aatatttccc gaacaatctg gcgagtccct
      781 tggactgttc ttctgaaaac tgcatctctt tcaacttctc ataagtttcg agcgtattca
      841 tatcaatcat acttagacct gtaaaaaaaa tgtcaacttc tcattttttt cttcgctgaa
      901 atacacctat ggtcaaatta aattttggtg gtgaaaatga aaaatcagaa cagattgact
      961 tcggcgatcc gggattgaac ctgttactgt gaaccggaca gctgacttat tctgggaact
     1021 gatccattcc ggaagaccga aggaaatgaa cattcttctg gatcagattt ccatttcagt
     1081 aaaagacttc ggttccgagg actggattca gctggatgaa gcggtcagaa aatcctctcc
     1141 ctactatgca tctttgaata tgagggaaaa aaatcctgcg gaatttgttc cggaaaacag
     1201 cacattggaa aaacaggttc ttctgcttgg actctgttct atgcatccgg taggattttt
     1261 ccgggagaag gcactgaatc ttctgggaaa agtaagggac gggtcggaac ttccttttct
     1321 tcttctgcgt ctgaaggact gggcagggcc tgtcagatcc gctgcagaga aaatgctcag
     1381 ggaaagactt tcttctgaat acagcagtta ttatattcaa ttttatcatc ttttatgact
     1441 attaaaatat tattatcaga taactcatat atac
//
LOCUS       DAOQGJ010001241         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66579_length_1474_cov_2.744891, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001241 DAOQGJ010000000
VERSION     DAOQGJ010001241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66579_length_1474_cov_2.744891"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..>1474
                     /locus_tag="PK683_16795"
     CDS             417..>1474
                     /locus_tag="PK683_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10158.1"
                     /translation="MKLLSAILVTLLLSAGDRLYADTVTRKSGAVLSNVKAFIKKGFI
                     QVEFEDLSKTDFSKKDIKSVKFSPVVWKPKIPAKDEKTSAPVPAELETSEQERIREAT
                     VNGDEWQERPESEQISPFKNALLGLIPGYSGLYRTDNKLGGAVFTFLEITALLNYANI
                     AGAKRQDIRYTQFLQDRQFGIFNLGLYNDNAGRGGTADTLINSFNSYYIQGFLLLKYR
                     SNYIGAVDGKEILSAATGAELKNQRTTAAVILGAVLAADAVASYLAASSWNEGIFQGA
                     KTARPTTRLSRFIRSALFPGWGQMYGGNDLKGLGYFAMGGMLLANVIQKEVAAVQAMN
                     DFNSYNFLNRSINFLAVN"
BASE COUNT          457 a          290 c          295 g          432 t
ORIGIN      
        1 tcagtctcct tcacgaccat tttttgtcgt tactaaaaat gggggtatga ccaaattctc
       61 catgaatcaa aaaatgacat taccctagac ttatcgaagc atctttaatt cgacaggaag
      121 catacgtgca ctcctaatat aatctatata tattatgaga gttaatttta tatttttaaa
      181 gcaatcctct cattcgcata atcacagatc cggaactaag cagtgatttt ttatgaactg
      241 cactaaaata aattgactga attttcaatt aatatgattg caatacagct tatattttat
      301 ttttccaagc acttctgaaa attaaacctc aactgataaa taattttttc tttacgtttt
      361 cctttacact attctatatg ttgttttgat taatttttaa atatcaaaga ggaagtatga
      421 agttactttc agccattctg gtaaccctgc tcctttctgc aggtgaccga ctgtatgcgg
      481 atacagtcac caggaaatcc ggtgcagttc tgtcaaacgt aaaggctttt attaaaaaag
      541 ggttcatcca agttgaattc gaagaccttt cgaagactga tttctccaaa aaagatatca
      601 agagcgtaaa gttttctcct gttgtatgga aaccgaaaat tcctgcgaaa gatgaaaaaa
      661 catctgcacc cgttcctgcc gaactggaga cttccgaaca ggaaaggata cgggaagcca
      721 ccgtgaatgg agatgaatgg caggaaagac cggaatcaga acagatcagt ccttttaaaa
      781 acgcactgct tggactgatt ccaggataca gcggtttgta ccgaacggac aataaactgg
      841 gcggtgcagt gtttactttt ctcgaaataa ccgctcttct gaactatgcc aatatcgcag
      901 gagcaaaacg gcaggatatc agatatacac agtttcttca agacaggcag tttggaatat
      961 tcaatttggg cctctacaat gacaatgctg gaagaggagg cactgcagat acgctgataa
     1021 attcctttaa ttcctattat attcaaggat ttctccttct taaatacagg agcaattata
     1081 taggtgctgt tgacggtaaa gaaattctat cagccgcaac gggtgcagaa ttgaaaaatc
     1141 aaagaacaac agcagcagtt attttgggag cagtactggc agctgatgcc gttgcttcct
     1201 atctggccgc atcctcctgg aatgaaggga tattccaggg agcaaagact gcaagaccga
     1261 ccacacgact ttcccggttt attcgatccg ccctgtttcc gggatgggga cagatgtatg
     1321 gcggaaatga tctcaagggt ctgggctatt ttgcaatggg aggaatgctt ttggcaaatg
     1381 tcatccagaa agaggtcgct gcagtgcagg caatgaatga tttcaattct tataatttcc
     1441 taaatcgctc tattaatttt ttagctgtaa atca
//
LOCUS       DAOQGJ010001242         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66666_length_1473_cov_2.489422, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001242 DAOQGJ010000000
VERSION     DAOQGJ010001242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66666_length_1473_cov_2.489422"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1473
                     /locus_tag="PK683_16800"
     CDS             <1..>1473
                     /locus_tag="PK683_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10159.1"
                     /translation="MKRKKTFILLLFSLNFCLQTEKFSFDFKNNPLFGIGFAVWSDAN
                     SKYTLGGSFKGFSSGSLILTLNGTDETFAPSSNYTFSQKLSSGTAYTVTVKTNPTGYV
                     CSVSNGSGTLGSSSVTNINLSCALITVTAAYTTNGSNWNDYINRDFTKDIFAQTDTAC
                     NTANTGGYRTCVHAGEIRKMDLPSRTSCTTLTASDDQSALNWVCRTNTSGTVTFYSSG
                     LKKGKYLSDLIDWTTNSWKSLTVTVKDGTATHAVSSALKLWSNVIDASETGSPSIAGT
                     VYLYKTSPNAAINISSSTNKIAVLLKPGTVFRLGTASNAMDFHGMFNWVEGSLMNNTN
                     ATGLLMNTRFSAVKNFRTQNIGKLSTNSYGISVSGNYGNNLLEDINTSNTGGGVLNYG
                     IYFTSSKDNIIISPVSANNQGDGINFDTASSGNSIIGAVAFNNGGSGVLFGGGLLTDS
                     FVADITSVNNTTDGLNASGAYQMMTNIASVNNGSNGLYGSP"
BASE COUNT          457 a          290 c          332 g          394 t
ORIGIN      
        1 atgaaaagaa aaaagacttt catcctgctg ttattttctc tgaatttctg tcttcagacg
       61 gaaaaattca gttttgattt taagaataat cctctgttcg gtattggatt tgcggtatgg
      121 agcgatgcaa actcaaaata tactcttggg ggcagcttta agggattcag ctccggatct
      181 ctgatactga ctttgaacgg gacagatgag acatttgcac cgtcttccaa ttatactttt
      241 tcacagaagc tttcttccgg cacggcatac actgtaacag tgaaaaccaa tccgacagga
      301 tatgtctgca gtgtttcgaa cgggagcggg acactgggtt cttcaagcgt aacgaatata
      361 aatctgagct gtgctctgat tacagtgacg gcggcatata cgacaaacgg gtcaaactgg
      421 aatgattata tcaacagaga ctttactaag gacatatttg cgcagacaga tacagcctgc
      481 aatactgcaa acacgggagg atacagaacc tgtgtacatg caggagagat ccgaaaaatg
      541 gatctgcctt ccagaacttc ctgtacgaca ctgactgcct cagatgatca gagtgcactg
      601 aactgggtct gcagaacaaa tacatccggc acagtgacat tttattcctc aggactgaaa
      661 aagggaaagt atctttctga tctcattgac tggacgacga attcctggaa aagtctgact
      721 gtcacagtaa aggacggaac agcaactcat gcagtcagca gtgcattaaa attatggagt
      781 aatgtgattg atgcgagtga gacaggttca ccctcaatag cgggaaccgt ctatctttat
      841 aaaaccagtc caaatgccgc aatcaatatt tccagttcaa caaacaaaat agcagttctt
      901 ctaaagccag gaactgtatt tcgtctggga acagcatcaa atgcaatgga ctttcacgga
      961 atgttcaact gggttgaagg ttcacttatg aataatacta atgcgacagg tctgctgatg
     1021 aatacccgtt tttctgcagt gaaaaatttc agaacgcaga acattggaaa attaagcacc
     1081 aattcctacg gaatcagtgt cagtggaaac tacggcaaca atcttctgga agatatcaat
     1141 acgtccaata cgggaggagg tgtgttgaat tatggaattt atttcacatc gtcgaaagat
     1201 aatataatta tttcacctgt atcggcaaac aatcagggtg acggaataaa ttttgacact
     1261 gcatcgtccg gaaattctat aattggtgca gtggcattta ataatggggg gagcggggtt
     1321 ttatttggag gaggcttgtt gactgacagt ttcgttgcag atatcacttc tgtaaacaat
     1381 actactgacg gtctgaatgc atccggagcc tatcagatga tgacgaatat tgcatctgtc
     1441 aacaatggct cgaatggatt atacggaagc cca
//
LOCUS       DAOQGJ010001243         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66850_length_1471_cov_1.748588, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001243 DAOQGJ010000000
VERSION     DAOQGJ010001243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66850_length_1471_cov_1.748588"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PK683_16805"
     CDS             complement(<1..1444)
                     /locus_tag="PK683_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNH10160.1"
                     /translation="MLSVLFYCSSEARKEAIIPVREEMKSEQEKSPAIAPPPKPTGAA
                     VQDIKRKNNKEESDGKIPDKKKTDSFNSFAENPFVSTSKEKFSTFSLKVDTASYSMIR
                     SFVNQGQKPNKDSVRIEEIVNYFPWKYRNDAQEKSPIYLHTEMTDCPWNKENALLRVI
                     FQTREIPASEIPPMNLVFLLDVSGSMSDANKLPLLKKSLRKVIDKMRDDDKIAIVVYA
                     AAEGLALPSTSGKDRDKILSALNKLESGGSTNGGAGITLAYKVAEENFIKDGVNRIIL
                     GTDGDFNVGVTGKNLVDMIAKKRETGIYMTLLGLGMGNYKDDTMENISKEGNGNYFYI
                     DTEAEADKVFVENFSATLLTVANDTKLQVEFNPQTVISHRLIGYENRMLATKDFKDDK
                     KDAGEVGPGHIVTAFYELKLKGKESDFGTVRSRYKIPPSNEVKEISAKLPRRKAAFEE
                     ASPEFHFASGAVAFGLILRDSKFKGKADWKL"
BASE COUNT          357 a          320 c          293 g          501 t
ORIGIN      
        1 gcagtttcca gtctgctttt cctttgaatt tagaatctct taaaatcagt ccgaatgcaa
       61 ccgcgcctga agcgaaatga aattctggag atgcttcttc aaaagctgcc tttcttctgg
      121 gaagttttgc agaaatttct tttacttcat tggaaggagg aatcttgtat ctggaacgga
      181 ctgttccgaa gtctgattct ttgcctttca gtttcagttc ataaaacgca gttacaatat
      241 gacccggtcc gacctcgccg gcatcttttt tatcgtcctt gaaatccttt gtggctagca
      301 tcctgttttc gtatccgatc agtctgtgag agatgactgt ctgaggattg aattctactt
      361 gaagttttgt atcgtttgca acggtaagaa gtgttgcaga aaaattttcg acaaaaacct
      421 tgtctgcctc tgcttccgtg tcaatgtaaa aataattacc gtttccttct tttgagatat
      481 tttccattgt atcatctttg tagtttccca tacccagtcc gagaagagtc atatagatgc
      541 ctgtctctct ttttttggca atcatatcca ccaggttttt tccagtgact cctacattga
      601 agtctccgtc tgttccgagg atgatcctgt tgactccgtc cttgatgaaa ttttcttcag
      661 caactttata ggcaagtgtg atacctgccc ctccgtttgt tgagcctcct gattccagtt
      721 tattcagagc tgatagaatt ttatcacggt ctttcccgga tgtagaagga agagcaagtc
      781 cttccgctgc tgcgtacacg actatagcaa ttttatcatc gtctctcatc ttatcaatga
      841 ccttccggag tgatttcttc agaagaggca gtttatttgc gtccgacata gaaccagaga
      901 catccagaag aaacaccaga ttcatcggag ggatttcaga ggcagggatc tctcttgtct
      961 gaaatataac ccgcagaagt gcattttctt tgttccaggg gcagtctgtc atttcagtat
     1021 gcagatagat cggtgatttt tcctgagcat cattcctgta cttccaaggg aaataattta
     1081 caatttcttc aatccgtact gaatccttgt tcggtttctg tccctgattt acgaaacttc
     1141 ggatcatgga ataggaagct gtgtccactt tcagagaaaa tgtactgaac ttttccttgg
     1201 aagtcgaaac aaaagggttt tctgcaaatg aattgaagga atctgtcttc tttttgtccg
     1261 gaattttccc gtcactttct tctttattgt tctttctctt gatatcctga acagccgctc
     1321 ctgtcggttt tggaggagga gcgattgcag gacttttttc ctgctctgat ttcatttctt
     1381 ctctgactgg aattattgct tcctttcgag cttctgaact gcagtaaaac agtacactga
     1441 gcattgagaa atgcagaaac gcaaataaaa c
//
LOCUS       DAOQGJ010001244         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66885_length_1470_cov_3.154064, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001244 DAOQGJ010000000
VERSION     DAOQGJ010001244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66885_length_1470_cov_3.154064"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK683_16810"
     CDS             <1..214
                     /locus_tag="PK683_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10161.1"
                     /translation="ISDAPKMSLSRDFLGLSEMQENFPALQKIHFLRTCKNNKVLSQA
                     AAQKTLQCLSQIILLKGSFMISFSFR"
     gene            238..762
                     /locus_tag="PK683_16815"
     CDS             238..762
                     /locus_tag="PK683_16815"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH10162.1"
                     /translation="MEKREINLPESERLKFREMSEKDLDFVHSMLSSPIVMKYYPKVF
                     TADDSRQWILRQNLRYDAYGTGLWIASDKKTEKPVGQMGLVFQTLSFGTELEIAYLLS
                     PEYFKTGYAAEAAKCLKEFAFQKMGRKFVISIIRPENKESRKVAERNGMSVWMEERFH
                     GFSHLVYRADPVLK"
     gene            complement(735..1121)
                     /locus_tag="PK683_16820"
     CDS             complement(735..1121)
                     /locus_tag="PK683_16820"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH10163.1"
                     /translation="MKEYSYENLKIFSKTEIINDARVRILVMEGKINNQNSHIVSADM
                     EMEWDDDVFNAIVDMTKLEYINSMGLAAILSMIEKNSENRGKFLIGGMNHYIEKIIQL
                     VEVSNKVEIFSSLQEAVNYFKTGSAL"
     gene            complement(1126..>1470)
                     /locus_tag="PK683_16825"
     CDS             complement(1126..>1470)
                     /locus_tag="PK683_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10164.1"
                     /translation="GSIETSGKLRSTVSIHSSENPHLDIFLDTGEILVTYQVPLDSLK
                     LFDSGSSFLCRKGADHRKIYLDFSGTLSLRRGEVRIIWQGEYLNLEKTFFEEMFLQRK
                     DNVLILSLKADT"
BASE COUNT          458 a          298 c          269 g          445 t
ORIGIN      
        1 catttccgat gctcctaaaa tgagtcttag tagagatttt ttgggcttat cagaaatgca
       61 agaaaacttc cccgctcttc aaaaaattca ttttttacgg acgtgcaaga acaataaggt
      121 tttatctcag gctgccgcac agaaaaccct ccagtgtcta tctcaaatca ttctgctgaa
      181 aggaagtttt atgatttcat tttcattccg atgagcaaaa aatgatttct gaactgtatg
      241 gaaaaaaggg aaataaatct gccggaatct gaaagactga aattccgtga aatgtcggaa
      301 aaggatctgg actttgtaca ttccatgctc agcagtccga tcgtaatgaa atattatccc
      361 aaggtcttca cagcagatga ttccaggcag tggatactga gacagaatct tcgatacgat
      421 gcttatggaa caggactttg gattgcctca gataaaaaaa cagaaaaacc agtaggtcag
      481 atgggactcg tctttcagac tctgagcttc ggaacagaat tggaaattgc ctatcttctt
      541 tcaccggaat atttcaagac cggatatgcg gcagaagcgg caaaatgtct gaaggaattt
      601 gcctttcaga aaatgggcag gaaatttgta atttccatca ttcggccaga aaataaagaa
      661 tcaagaaaag tagctgaaag aaacggaatg agtgtatgga tggaggaaag atttcacgga
      721 ttcagccatc tcgtttacag agctgatccg gtcttaaaat agttcacagc ctcctgcaga
      781 ctggaaaaga tttccacttt gtttgaaact tccacaagct gaataatctt ctctatataa
      841 tgattcattc cgcctatgag aaactttcct ctgttttctg aatttttttc tatcattgaa
      901 agaattgcgg caagccccat actgttaata tattcaagtt tggtcatatc tacaattgca
      961 ttgaatacat catcatccca ttccatttcc atatctgcag atacgatatg agaattctga
     1021 ttgtttattt tgccctccat aactaaaatt cttactctgg catcgttgat tatttctgtc
     1081 ttggaaaaaa ttttcagatt ctcgtaagaa tactctttca tattttcagg tgtccgcttt
     1141 taaactcagg atcagtacat tatcctttct ctgcaagaac atttcttcga aaaatgtttt
     1201 ttccagattt aaatactccc cctgccaaat gattcggact tcccccctcc tcagggaaag
     1261 ggttccggaa aaatccaggt atatttttct atgatctgct ccttttctgc ataaaaagga
     1321 agatccagaa tcaaagagtt tcagactgtc cagaggaacc tggtaagtca caagaatctc
     1381 ccctgtatcc agaaaaatat ccaaatgggg gttttctgaa ctgtgaatcg aaacagtaga
     1441 tctcaatttt cctgaagtct caatactacc
//
LOCUS       DAOQGJ010001245         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66897_length_1470_cov_2.684806, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001245 DAOQGJ010000000
VERSION     DAOQGJ010001245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66897_length_1470_cov_2.684806"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK683_16830"
     CDS             complement(<1..406)
                     /locus_tag="PK683_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNH10165.1"
                     /translation="MGSAAPGKSFFITTLGCPKNVADSEHVRLSLMSEGLEEAKSPDR
                     SDFHLINTCTFIQSATEETIDTIIQAGKIKKKRKQKLIVLGCFAERYSDAIRSDIPEV
                     DLSFGTGKYSSAGSILKSHFPEEFNGLQEINRD"
     gene            complement(409..>1470)
                     /locus_tag="PK683_16835"
     CDS             complement(409..>1470)
                     /locus_tag="PK683_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH10166.1"
                     /translation="VRDIVKETNIPLKYVLALENEDYGQFPAETYTVGFLKTYADFLK
                     LDTGHIINLFRGEQLEDANIPLQELTKPTMKMMAVEVRRNKAILPLILVLIVGIGVFA
                     FSQFYEPEQENSNPSGAGENENRYLQGVPSDISFINQSVHDSERVPFTLTPEQGFTFS
                     VNNQLCKIFIKGVRKNEDNENIAIIGFNLFPDKKVYTFETKVNENSVLSSKNPELSNL
                     RREIKILTQAITEKSAKILVSLGEAKPATEQGSGNNPVGDVPIQVTLFFGKSTFAEFL
                     IDGQGGERGIMPAGETKQLEAMSRLEIKVGDGGAVDIVQNGKPKVKMGKPNRHVKKIF
                     VKTPNRFDNTQFEIKELGE"
BASE COUNT          364 a          345 c          285 g          476 t
ORIGIN      
        1 tgtctctgtt gatctcctga agaccgttaa actcctcagg aaaatgagat tttaggatgg
       61 atcctgcact ggaatatttt cctgttccaa aacttaaatc cacttcaggg atatccgacc
      121 ggatggcatc cgaataacgt tcggcaaaac atccgagtac gatcagcttc tgctttctct
      181 tctttttaat ctttccagcc tgaatgatcg tatctattgt ctcttcggta gcggattgaa
      241 tgaaggtaca ggtattgatc agatgaaaat ctgatctgtc cgggcttttg gcctcttcca
      301 gtccttcact catcagagaa agccttacgt gctccgagtc agccacattt ttaggacatc
      361 ctagtgtggt aatgaaaaat gattttcccg gtgcggctga acccaaaatt actctcccag
      421 ttccttgatt tcgaactgtg tattatcgaa acggttaggt gttttgacaa aaatcttttt
      481 tacatgtcgg ttcggtttcc ccatttttac tttaggtttc ccgttttgaa cgatatcaac
      541 agccccgccg tctccaacct tgatttccag tctgctcatt gcttcgagct gctttgtctc
      601 gcccgctggc atgatgccgc gctcccctcc ctgtccatcg atcagaaact ccgcaaatgt
      661 ggatttccca aaaaacagtg tcacctggat cggaacatca cccactgggt tgtttcctga
      721 tccctgctcc gttgcaggtt tggcctcgcc tagagaaaca agtatctttg ccgatttttc
      781 tgtgatcgcc tgagtgagta tttttatctc tcttctcaga ttggaaagtt caggattttt
      841 actgctcaga acggaatttt cattcacctt tgtttcaaaa gtgtaaacct tcttatccgg
      901 aaacagattg aacccaatga tcgcaatgtt ttcattatct tcattttttc tgactccctt
      961 gatgaaaatc ttgcagagct gattgttcac gctgaaagta aatccctgtt ccggagtgag
     1021 tgtaaaaggg accctttctg aatcatggac tgactgattg ataaaagaga tgtctgaagg
     1081 aactccctgc agatatctgt tttcattttc tcctgctccg gacggattgg aattttcctg
     1141 ttcaggctca tagaactggg aaaaagcaaa tacaccgatt cctacgatca aaacgaggat
     1201 cagaggaaga attgctttat ttcttctcac ttccactgcc atcatcttca ttgtaggttt
     1261 agtcagttcc tgaagaggaa tatttgcatc ttcgagctgt tccccccgaa acagattgat
     1321 gatgtgacct gtatcgagtt tcagaaaatc agcatacgtc tttaaaaacc ctactgtata
     1381 tgtttctgcg ggaaattgac cgtaatcttc attctccaat gcaagcacat actttaaagg
     1441 aatatttgtt tcctttacaa tgtctcgtac
//
LOCUS       DAOQGJ010001246         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66955_length_1469_cov_3.473126, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001246 DAOQGJ010000000
VERSION     DAOQGJ010001246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66955_length_1469_cov_3.473126"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..>1469)
                     /locus_tag="PK683_16840"
     CDS             complement(405..>1469)
                     /locus_tag="PK683_16840"
                     /inference="COORDINATES: protein motif:HMM:NF013510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10167.1"
                     /translation="QPDLKLAIWADGMTFDEAAARLTEKPKKELSIQEEADKKRIALE
                     YKGQDILSVYNKYFSSQEKFTDLSNMEKMREYLANFPDEAAKIPDMLLKGQIRGPQVM
                     EIIHLLASVGHREAQSAIVKIFSSPTQSANAKMQALAGVHEIRYPNDETLGALWSVYE
                     DRKTEQNRDYSNTAVLALGSAASNLKKKEASAKIKERITAEIKAADKDKDSSRTAVLI
                     NSAGNTGDSNLLPEIKKYVSSEDKIVRTSLYDSLGNFKDKESLNLLQNGIEKETSPEA
                     RTKIMQSLLRRPADDETVKKAVSVLPKEQDPNTRNYILQYMIQNRKSISDFKEKMKAA
                     LEKENFENNRDLILKAMHSE"
BASE COUNT          337 a          332 c          298 g          502 t
ORIGIN      
        1 aatcatcaat agtatatata atatatattt catattattt tagtcaagcg tatatctcag
       61 atttctaatc tcgcaagtat ggatgccgct gctgcgctcc gcccttcggg ctattagaaa
      121 tctggaaaga gtctggagtc ccgcccttcg ggctgccgga caggcccaaa gggggttttg
      181 caaacccctt tgcggcggct cggaaacagc ctaatccccc gtgtttgctc acattcagtg
      241 agaatgcaga ggcattttca aacaatgaat aattgactat atgaaaattt cattggaaat
      301 ctaggtaata cttatcggtt tatcccattc tgcaaagagc tgcagaatca gaaaatgccg
      361 ccgacaggtt tttcctgcca gtgcatttcc gcgcattcag aattttattc tgaatgcatt
      421 gcctttaaaa tcagatctct gttgttttca aaattttcct tttcaagagc ggctttcatc
      481 ttttccttga aatcggaaat actctttctg ttctgaatca tgtactgcag aatataattc
      541 cttgtgttcg gatcctgttc cttcggcaga acactgactg ctttttttac agtctcatcg
      601 tctgcaggtc ttctcagaag gctctgcata atctttgttc ttgcttcagg ggatgtttct
      661 ttttctatcc cgttttgaag cagatttaat gattctttat ccttgaaatt tccaagactg
      721 tcgtaaagag atgttcttac gatcttgtct tctgaggaaa catatttttt gatttccgga
      781 agcagattgc tgtctcctgt atttcccgca gaattgatca gaactgcagt cctgctgctg
      841 tctttgtcct tgtctgcagc ttttatttct gctgtaattc tttccttgat tttagcggaa
      901 gcttctttct ttttcagatt ggatgcagct gaccccagag cgagcactgc agtgttggaa
      961 tagtctctgt tctgttccgt ttttctgtcc tcgtaaactg accagagtgc tccgagagtc
     1021 tcatcgttcg gataacggat ttcatggact ccggcaaggg cctgcatttt tgcatttgct
     1081 gactgagtcg gactgctgaa gatttttact attgcagact gagcttccct gtgaccgaca
     1141 gatgccagaa gatggatgat ttccattacc tgagggccgc gtatctgtcc tttcagaagc
     1201 atatcaggga tttttgcagc ctcatccggg aaatttgcca gatactctct catcttttcc
     1261 atattggaaa ggtcggtaaa cttttcctgg ctggaaaaat acttattata aacagaaaga
     1321 atatcctgtc ccttatattc cagtgctatt ctttttttat ccgcctcttc ctgaatgctc
     1381 agttcctttt tgggcttttc tgtaagacgg gctgccgctt catcaaaggt cataccgtcc
     1441 gcccagatgg caagtttcaa atcaggctg
//
LOCUS       DAOQGJ010001247         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_66956_length_1469_cov_3.397454, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001247 DAOQGJ010000000
VERSION     DAOQGJ010001247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66956_length_1469_cov_3.397454"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="PK683_16845"
     CDS             <1..1351
                     /locus_tag="PK683_16845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH10168.1"
                     /translation="AGFLLRIPSANELTGIRKERRPKDWAEFVLASSEQPEMKNDTRT
                     HILVVDDEVSVLNLVRNYLETNGFRVSTAVTADEGVVKAGTLLPNLVLLDLGLPGSNG
                     YEVTQQIRRKHSFSHMPIIIMTVRETMEEALQGLEFGANDFILKPISVQNLVARVKIC
                     LKISDINGSYERFVPPELVKLLKKQNITDVFLGDHVEREITILFSDIRSFTKISEEMT
                     PGETFSFLNEYLERVGPVIKRNRGFIDKYIGDAIMAIFPFSADDAVRAAIQMQKEIYQ
                     LNAELFAAGKKTIRVGIGIHTGDVILGTIGEEERMEGTVISDAVNLTSRLENLTKLYG
                     TNILISMDTFLNLDVPEEYNFRILDRVKVKGKNKLVTVIEIFDGLEPERMGMAVSTKT
                     LFEEAVAHYLSRDFQTAIDGFMQILDVNPKDNAAMIYVQRAEYYMNSVLPENPIQSR"
BASE COUNT          452 a          295 c          365 g          357 t
ORIGIN      
        1 agcgggattt cttctgagaa ttccttcagc aaatgaactg accggaatca ggaaggaaag
       61 aagaccgaaa gactgggcag aattcgttct ggcatcctct gaacagccgg aaatgaaaaa
      121 tgataccaga actcatatac tggttgtgga cgatgaagtc agtgtactca accttgtgcg
      181 caactacctg gaaacgaacg gattcagagt gagcactgca gttactgcag atgagggagt
      241 tgtcaaggca ggaaccctcc ttccgaatct ggttcttctg gatctgggcc ttcccggatc
      301 aaacggttat gaagtcactc agcagatccg cagaaaacac agtttcagtc atatgcccat
      361 catcattatg actgtcagag aaacaatgga agaagcactt cagggactgg aattcggtgc
      421 gaatgatttt atactgaaac cgatatcagt gcagaacctg gttgcaagag tgaagatctg
      481 tcttaaaatt tctgatatta acggttctta tgaaagattt gttccgcctg aactggtaaa
      541 acttctgaaa aagcagaaca ttactgatgt tttcctcgga gatcacgttg aaagagaaat
      601 tacgattctg ttttccgata tcagatcttt tactaagatc tcggaggaaa tgactccggg
      661 agaaaccttc agttttctga atgagtatct ggaaagagtc ggtcctgtca ttaaaagaaa
      721 cagaggattc atagacaagt atatcggcga cgcaattatg gcgatatttc ctttttctgc
      781 agacgatgct gtccgtgcag caatacagat gcagaaggaa atctatcagc tgaatgcaga
      841 acttttcgct gcaggaaaaa aaacaatcag ggtggggata ggaattcata caggtgatgt
      901 gattttagga acaatcggtg aagaggaaag gatggaaggg acagtcatca gcgatgcagt
      961 gaaccttact tcaaggctgg aaaacctcac caaactgtac ggtacaaaca ttctgatcag
     1021 tatggataca tttctgaatc ttgacgttcc tgaggaatac aatttcagga tactcgacag
     1081 ggtgaaagta aaaggaaaga ataagctggt gaccgtaatt gagatatttg acggtctgga
     1141 accggaaaga atgggaatgg cagtctctac aaaaactctg tttgaagaag ctgtggcaca
     1201 ttatctttcc cgggattttc agacagcgat tgacgggttt atgcagattc tcgacgtaaa
     1261 tcccaaagac aatgctgcga tgatttatgt tcagagagcg gagtattata tgaattctgt
     1321 actgcctgaa aacccaatac agagcaggtg attccgaaaa ctgtatgcgc ggtgaggcca
     1381 gcacagcgcc caccgccagg ccacgcgtgc acgaagccga cgagggcgtc ggcgctccca
     1441 gcaaccgcgc tcgctggatt caaaggctt
//
LOCUS       DAOQGJ010001248         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67032_length_1468_cov_3.893843, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001248 DAOQGJ010000000
VERSION     DAOQGJ010001248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67032_length_1468_cov_3.893843"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK683_16850"
     CDS             <1..189
                     /locus_tag="PK683_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10169.1"
                     /translation="TSDARPSGSIKDLHFLDTQSNLFETADSLPELTSLVHLDISDIQ
                     ICGFRPLEKLVNLTELVN"
     gene            complement(200..850)
                     /locus_tag="PK683_16855"
     CDS             complement(200..850)
                     /locus_tag="PK683_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH10170.1"
                     /translation="MQDYKDSLKFKNWLDSLKKNNIEIQNITEKAVIHKSNGEVLFCL
                     LNVDAKDSAGQKLLPTVLIRGHFVSVVTCLKEKETGKRYFLLVKQRRVGNGEIFYEHP
                     AGMCDSETDPYKVAEKEVEEETGLKIEKKDLELLWETPLYSSPGLLDEAGYFFCCEIS
                     LSAEEIHSFRDRLTGAEHEHEFIQTYVCESDEIFKLLKNSNGIMATLLFQKKKGIN"
     gene            complement(881..>1468)
                     /locus_tag="PK683_16860"
     CDS             complement(881..>1468)
                     /locus_tag="PK683_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNH10171.1"
                     /translation="KEILDSIDRKDSAGIRDYAINVMKYLTALRDVEISRANIEDIRV
                     SSGEYILHIHGKGRTETDRSVEFIILKEPVYKAVIEYLHSGGRTLDSKGPLFISESKR
                     NKKKRISVKTVQRAIFDPIKRLGYKTHRITSHSTRHTAISLAYEAGASQEELMEFARH
                     KDPKTTRIYTHKKNRFQSNAETKLTDFFLKTDSTD"
BASE COUNT          361 a          310 c          292 g          505 t
ORIGIN      
        1 acttccgatg caaggccatc aggttcaata aaagacctgc atttcctcga tacacagtct
       61 aatctgtttg aaactgcaga ttcattgcca gaactgacat ctcttgtcca tctggatatc
      121 agtgacattc agatttgcgg tttcagacct ttggaaaaac ttgtgaatct gactgaactt
      181 gtaaactaaa tcgcttaact caattgatcc ccttcttttt ctgaaagagc agggtggcca
      241 taatcccgtt tgagtttttc agaagtttga agatttcatc tgattcacat acgtatgtct
      301 gaatgaattc atgttcatgt tctgcacctg tcagtctgtc tctgaaggaa tggatttctt
      361 ccgcgctcaa ggaaatttca cagcagaaaa aatatcctgc ttcatccagg aggccgggag
      421 aactgtaaag aggagtttcc caaagaagtt ccagatcttt tttttcaatc ttcagtccgg
      481 tctcttcttc cacttctttt tctgccacct tgtatggatc agtttcactg tcgcacattc
      541 cggcggggtg ctcatagaaa atttctccgt ttccgactct tctctgcttt acgaggagga
      601 aatatctttt tccggtttcc ttttccttga ggcaggtgac aaccgagaca aaatgtcctc
      661 tgataagaac agtggggaga agcttctggc ctgcagagtc ttttgcatct acattcagaa
      721 gacagaaaag aacctcgccg ttggatttat ggatgacagc tttttcagtg atgttctgaa
      781 tttcgatatt gtttttcttc agtgaatcga gccagttttt gaatttaaga gaatccttgt
      841 agtcctgcat aaggataggt aaaatcagaa agaatgaatg tcaatctgtt gaatctgtct
      901 tgaggaaaaa atcagtcagt ttggtctctg cattggactg gaatctgttt ttcttgtgag
      961 tataaattct tgtcgtcttc ggatccttgt gtcttgcgaa ttccatcagc tcttcctgac
     1021 ttgcgcctgc ttcatatgcc agtgaaattg cagtatgtct tgtactgtgt gaagtaattc
     1081 tgtgagtttt atatcccagc cttttgatcg ggtcgaagat tgctctttga actgtcttta
     1141 cagaaattct tttctttttg ttccttttgg attctgaaat gaaaagagga cctttggagt
     1201 caagtgttct tccgcctgaa tgaaggtatt caatcactgc cttgtataca ggttctttca
     1261 gaatgataaa ctctactgat ctgtccgttt ctgtcctgcc tttcccgtga atatgaagta
     1321 tgtactctcc tgaactgaca cggatatctt caatattggc tcttgaaatt tccacatccc
     1381 tgagtgcagt cagatatttc atcacattga tagcataatc cctgatgcct gcactgtctt
     1441 ttctgtcaat gctatccagg atttcctt
//
LOCUS       DAOQGJ010001249         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67060_length_1468_cov_2.998585, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001249 DAOQGJ010000000
VERSION     DAOQGJ010001249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67060_length_1468_cov_2.998585"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..600)
                     /locus_tag="PK683_16865"
     CDS             complement(94..600)
                     /locus_tag="PK683_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10172.1"
                     /translation="MMVFLLLSVSCKSKDTSYRPAREDGTESSPSSGRELSEAQKKEL
                     PQMIEIPTIHIGENLKRIKNLSEWNSISEGFRSSYAYQVKVTSLKSDPELAIREAVDV
                     AKRKSAKMLIAEAIPGLSNDSKTDIKILVEEYGKIVADSDFFDNKHHFVFLILKPGLE
                     TIIKEKLK"
     gene            703..>1468
                     /locus_tag="PK683_16870"
     CDS             703..>1468
                     /locus_tag="PK683_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10173.1"
                     /translation="MKLFLFMILLSAVGCNPQLKRTVLDKAPISGILAILSSGTYSLN
                     VSVSGLVSPVEIKNHRSEILQISQNGKFQFPSKLSSGENYTVSISGTMSGQICSFDQS
                     SGTAGSGDAAVAMTCTASEILPFFANAPAWNDYVKNDGTSVQSDGKSYPNFFLASGTA
                     CAGTEQTYKGCFHGGFYRKIVLSSLTDCTGIRTEDNLGIFGWGCGNVNGKTVAVNYAF
                     SSGKNLADLVQFDSGASYFKENYVRVYKNNVLYYQTT"
BASE COUNT          417 a          289 c          308 g          454 t
ORIGIN      
        1 atttctgttt cgagccgatt ttagggtaga aaaggaattc tgaatgtttg gtaagcaggt
       61 tattttttca ggacttcaga gtcaatggta aatttatttt agtttttcct taatgatcgt
      121 ctccaggccc ggcttaagaa tgagaaatac aaaatgatgc ttattgtcaa aaaaatcaga
      181 gtcggcaaca atctttccgt attcttccac caggattttg atatccgttt ttgaatcatt
      241 ggaaagaccg ggaattgcct cagcaatgag cattttggcc gatttccttt tggcaacatc
      301 cactgcttcc ctgattgcaa gctccggatc tgattttaat gaagtgacct ttacctgata
      361 agcatagctg cttctgaatc cttcggaaat agaattccat tcggaaaggt tcttgattct
      421 tttcagattc tctccgatat gaatggtcgg gatctcaatc atttgaggaa gttctttttt
      481 ctgagcttct gaaagctccc ttcccgatga gggtgaagac tctgttccgt cttcacgggc
      541 aggcctgtag cttgtatcct tggacttgca ggaaacagaa agaagtaaaa ataccatcaa
      601 tagaaacttc ataattgtat ttttgcaatt aaaaaacgaa actgcaacaa gtttttagaa
      661 aaaaatgctg ttttaatttg tctaagagtg tatggactgt gtatgaaact tttccttttt
      721 atgattctgt tatccgctgt cggctgcaat ccccagctga aaagaaccgt actggataag
      781 gctcccattt ccggaatcct ggcaatacta tcttcgggaa catattcact gaatgtatct
      841 gtttccggtc ttgtttctcc tgtagaaata aaaaaccaca gatccgaaat tttacagatt
      901 tcacagaacg gtaaatttca atttccttca aaactcagtt ccggagaaaa ttacacagta
      961 tctatttcag gaaccatgag cggacagatc tgcagctttg atcagtcttc cggaacagca
     1021 ggctctgggg atgctgcagt ggcaatgacc tgcactgctt cagaaattct tccttttttt
     1081 gcaaatgctc cggcctggaa tgactatgta aaaaatgacg gaacctcagt tcagtctgac
     1141 ggaaaaagtt atccgaattt cttcctggca tcaggaaccg cctgtgccgg aacagaacag
     1201 acttacaagg gatgttttca tggaggattt tacagaaaga ttgtcctttc gagtttgact
     1261 gactgtacgg gaataaggac agaggacaat ttgggaattt tcggatgggg gtgcgggaac
     1321 gtcaacggta aaaccgttgc agtcaattat gcattttctt cagggaaaaa tcttgctgat
     1381 cttgttcagt ttgattccgg cgcatcctat tttaaggaaa actatgtcag agtgtacaag
     1441 aacaatgtac tgtactatca gacaacac
//
LOCUS       DAOQGJ010001250         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67070_length_1468_cov_2.828733, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001250 DAOQGJ010000000
VERSION     DAOQGJ010001250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67070_length_1468_cov_2.828733"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PK683_16875"
     CDS             complement(<1..269)
                     /locus_tag="PK683_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10174.1"
                     /translation="MEGMTVPGIRRGYGLARISLERTDVQTRNASLRTEIAAFRLSGS
                     SEKDSVFWLFYLLLELEKSGSLYLDLAEYSEFISGKGLVWNPDSLG"
     gene            complement(257..>1468)
                     /locus_tag="PK683_16880"
     CDS             complement(257..>1468)
                     /locus_tag="PK683_16880"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNH10175.1"
                     /translation="MHASKGLEFRVCILFNLFGKNRPDMGEIYYKDKKLSGSSAEVQF
                     RTAGMQTEGYEREHSSEAEELYSEKKRLLYVSMTRARDYLIFPMFVFGREGDGSFRMI
                     IRKFLDSKIPDLFRENLALNLSLYPERQKSDRPVLKKKTASKKAEFVEIPQEVFREKS
                     RKKIFSYSSLKSLDDQKRKKETETDQKPEESGLQKSQKNAGGIFGSLCHLVLENIPVN
                     SFVSRAKIKKAADFYAEKFYSLSGLQDDPGYSKNDLVQLCVNALDCRYEISGQKVRPS
                     EWENFRREKRFYHHIGNSRYSDYYMGIGDGIFTYDNKIYLLDWKSDRDHNEKSREDLL
                     AYTLDKYHYQILLYSLNIYENLKVLEKTYSDYGGILYIYLRNIGSGIPAVLVKPSVEE
                     IKKFERNPWKE"
BASE COUNT          402 a          351 c          244 g          471 t
ORIGIN      
        1 cccagtgaat ccggattcca gaccagtcct tttcctgaaa tgaactcaga atattctgca
       61 agatccagat agagactgcc tgatttttca agttccagaa gaagataaaa cagccagaaa
      121 acagaatctt tttccgatga tcccgaaagt cggaatgccg caatttcggt tcttaaagaa
      181 gcattccttg tctgcacatc agttctctca agactgattc tagcaagccc gtaacctctc
      241 ctgattccgg gaacagtcat tccttccatg gattcctctc aaatttttta atttcttcca
      301 ctgaaggctt cacaagcact gcaggaattc cgctgcctat atttctcaga tatatataaa
      361 gtatcccgcc atagtcggaa tacgtctttt cgagcacctt gagattttca taaatattca
      421 gactgtaaag aagtatctga taatgatact tgtccaaggt atatgccagc agatcctccc
      481 tgcttttttc attatgatcc ctgtccgact tccagtccaa aagatatatt ttattatcat
      541 aagtaaatat accgtcgcct attcccatat aataatcaga ataacgggaa ttcccgatat
      601 ggtgatagaa tcttttctct cttctgaaat tttcccattc tgaaggacgc accttctgtc
      661 cggatatttc atatctgcag tcaagagcat ttacgcagag ctgaacaaga tcatttttgg
      721 aataccctgg atcatcctgc agtcctgaca gagagtaaaa cttctccgca taaaaatccg
      781 cagctttttt tatttttgcc ctgctcacga acgaattcac cgggatgttc tcaagaacaa
      841 gatgacacag gcttccgaat attcctccgg catttttctg agatttctgc agaccagact
      901 cttcgggttt ctgatctgtt tcagtctctt tctttctctt ctgatcatcc agacttttca
      961 gtgaggagta ggaaaatatc ttctttctgc ttttttctct gaatacctcc tgcgggattt
     1021 ccacaaattc tgctttttta gaagcagtct tttttttcag gacaggcctg tctgatttct
     1081 gcctctcagg ataaaggctc agatttaatg caagattctc cctgaaaagg tctggaattt
     1141 tactgtccag aaatttacga ataatcattc tgaacgaacc gtccccttcc cttccaaaaa
     1201 caaacattgg aaaaataaga tagtccctag ccctggtcat cgaaacataa agaagtcttt
     1261 ttttctcaga gtaaagttcc tctgcctcag acgaatgttc cctttcataa ccttctgtct
     1321 gcattcctgc ggttctgaac tgaacttcag cagaactgcc ggataatttt ttatccttat
     1381 aatatatctc tcccatatcc ggacgatttt tcccgaaaag attgaataga atgcatactc
     1441 tgaattccag accttttgat gcgtgcat
//
LOCUS       DAOQGJ010001251         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67089_length_1468_cov_2.322718, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001251 DAOQGJ010000000
VERSION     DAOQGJ010001251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67089_length_1468_cov_2.322718"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PK683_16885"
     CDS             <1..780
                     /locus_tag="PK683_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10176.1"
                     /translation="ELVVLNVGYDLGILSSEIFSIMVLMALVTTFMTGPALDFINYIF
                     PDREKAESPVPEKSILFSFGKPEMGYKLLQTARLLSSDSGLKAVHITPNQYLLEEESV
                     LYREEAFRPVRDLSKSLNLELEEIYKVTENISSEILHQMKLHSPTLLLVGAAKSVLSD
                     DIFGGTLKKVLKESPVPVGIFLESENQDFRNIIVLKKEDSLQMKSLLENLKPSLPAVT
                     VSPEEFSEKTVMKDSLICMEFHDWNESFAGMSASFLVFHFP"
     gene            complement(783..>1468)
                     /locus_tag="PK683_16890"
     CDS             complement(783..>1468)
                     /locus_tag="PK683_16890"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH10177.1"
                     /translation="PRNYGKLIPDLRVIVVDFAHEFLNSVFRENMELLTNSCIEIQNF
                     RHLKNLSSLTEKLSEAENSQEKLNILNSTFAEFFCTLDLTQNEMSLRMMDAVNAAEGN
                     ISIRDLSRELDCSERTLRRKSIQAFGVSPVQYIRTVKAEFAIARLLFDEESDMQGLVF
                     DFGYYDQSHFIHDVQSAISLSPGTVRKIRHLPEMQSLRALLRHRHKGCNMHKRGSGEM
                     KIVFDKCKD"
BASE COUNT          378 a          324 c          319 g          447 t
ORIGIN      
        1 gagcttgttg tgctgaatgt gggatatgat cttggaatcc tgtcttctga gatattttcc
       61 attatggttc tgatggctct tgtcactacg tttatgaccg ggccggcgct tgatttcatc
      121 aattatattt tcccggacag ggaaaaagcg gaatcgcctg ttcctgaaaa gtccattctg
      181 ttttctttcg gcaagccgga aatgggatat aagctgcttc agacagcaag acttctttcc
      241 tccgattccg gtctcaaggc agtgcatatc actccgaatc agtatcttct ggaagaagaa
      301 tcagttctct acagggaaga ggcattccga ccggtcagag atttatccaa gagtctgaat
      361 ctggaacttg aagaaatata caaggttacg gaaaatatca gttctgaaat tctgcaccag
      421 atgaaacttc acagtccgac tcttcttctt gtaggtgctg caaagtccgt tctgtcggac
      481 gatatcttcg gaggcacact gaaaaaggta ttgaaggaat cgcctgttcc tgtcgggatt
      541 tttctggaat cagagaatca ggacttcagg aacatcattg tgctgaaaaa ggaggattct
      601 cttcaaatga agtctctgct tgaaaatctg aagccctccc ttcctgcggt gactgtttcc
      661 cccgaagagt tctctgaaaa aactgtaatg aaggattcat tgatctgcat ggaatttcat
      721 gactggaatg aatcttttgc cggaatgtct gcatcctttc tggtatttca ttttccttaa
      781 gatcagtctt tacacttatc gaatacaatc ttcatttcgc cgctgccccg tttatgcata
      841 ttgcagcctt tatgacggtg tctcagcagt gcgcgaagtg actgcatttc cggaagatgt
      901 ctgatctttc gaacagttcc aggggaaagg gaaatcgcac tttgaacatc gtgtataaaa
      961 tgggactgat cgtaataacc gaagtcgaat accagaccct gcatatccga ttcttcatcg
     1021 aacagcagtc ttgcaattgc aaattctgct ttgactgttc tgatgtactg aaccggtgaa
     1081 acaccgaatg cctggattga cttccttctc aaggtgcgct ctgaacagtc aagctccctg
     1141 ctcagatccc tgatgctgat atttccttct gctgcattga ctgcatccat catcctgagt
     1201 gacatttcat tctgagtcag atccagtgtg cagaaaaatt ctgcaaacgt gctgttcagt
     1261 atgttcagtt tttcctgact gttttccgct tcggaaagtt tttcagtcag agaggacaga
     1321 ttcttcagat gtctgaaatt ctgaatttca atgcaggaat ttgtcagaag ctccatattt
     1381 tcccgaaata ctgaattgag aaattcatgt gcaaagtcca ctacaatgac ccgcagatcg
     1441 ggaatcagct ttccgtagtt tctcggat
//
LOCUS       DAOQGJ010001252         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67133_length_1467_cov_3.070113, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001252 DAOQGJ010000000
VERSION     DAOQGJ010001252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67133_length_1467_cov_3.070113"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..740)
                     /locus_tag="PK683_16895"
     CDS             complement(219..740)
                     /locus_tag="PK683_16895"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH10178.1"
                     /translation="MKLGEKIVVTVSFLATVLGIAYITLEVKRKKKTPDYQDLSAYDL
                     HGKKIRLNNYQGRVLVVDFWATWCVPCRDAAVVVDKLYQKAPQDKFQFYGVNTDHNKT
                     LKEISDAALDFGMSYETLLDSQLVLAKKWSVEGLPTLIVFNREGKIIYRQMGIRPEEY
                     PLLLRKMQDWENE"
     gene            complement(744..1376)
                     /locus_tag="PK683_16900"
     CDS             complement(744..1376)
                     /locus_tag="PK683_16900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNH10179.1"
                     /translation="MVKHLYLVRHGETEFNLAGVIQGGGLDSPLTEKGHRQAVKLGEY
                     LRSNSFICDYIFASPLGRTMETARLAVSGLGRDIITDDLLKEIDCGDFEGRKIADIDL
                     EKLYRLRVDPKEKYPKGESVEDVRVRAERFIRGLKDYPGESAIIFSHGNFLRSFAAAA
                     AGLSSEFSMKIYIENGALCYLFKSSEHYRISVWNFTGHYAPFPKKIETSG"
BASE COUNT          385 a          363 c          300 g          419 t
ORIGIN      
        1 aggcctgccg gccaatgccg ccaaattaat acagcacagg agcaatgctg cggctcgctt
       61 tacaagccgc cgaagggtct cccattggaa atccctgccc tctgctttaa atttatgctg
      121 agcagttaca aaaaatgttt ctgcgaagcc ttgctccacc cctattcaaa atccggaggg
      181 cttgcaaatt ttcaatataa atttctccgt ttccgagatt actcattttc ccagtcctgc
      241 atttttcgga gcagaagagg atattcctca ggacggatac ccatctgtct gtagatgatt
      301 tttccttccc gattaaacac aatcagagta ggaagacctt ctacagacca ttttttggca
      361 agaaccagct gggaatccag aagagtttca taactcattc caaagtccag tgctgcatcc
      421 gagatctcct tcaaggtctt attatggtct gtattgactc cgtaaaactg aaatttgtcc
      481 tgaggggcct tctggtacag tttgtccact acaacggcgg catcacggca gggaacgcac
      541 caggtagccc agaaatccac tacaaggact cttccctgat aattgttgag tctgattttt
      601 ttaccatgca gatcatacgc ggaaagatcc tggtagtcag gagttttttt ctttctcttg
      661 acctccagag taatataagc aattcctagt acagttgcca gaaaagagac agtgactaca
      721 attttttcgc cgagtttcat tgtctatcct gaagtctcaa tttttttagg aaaaggtgca
      781 taatgacctg taaaattcca tacggaaatt ctgtaatgct cgctgctttt aaaaagatag
      841 cagagtgcac cgttctcaat gtagattttc attgaaaatt cggaagacag tcctgccgca
      901 gcggcagcaa aggaacgcag aaaatttccg tgagaaaaaa tgatggctga ctctccggga
      961 tagtccttca aacctctgat gaacctttcg gctctaactc tgacatcctc aacgctctct
     1021 cccttaggat atttttcctt aggatccacc ctgagtctgt acagcttctc cagatcaatg
     1081 tctgcaatct tcctgccttc aaaatcaccg cagtcaattt ctttcaaaag atcatccgta
     1141 atgatgtctc ttccaagccc ggagacggcg agccttgccg tttccatagt cctgcccaga
     1201 ggactggcaa aaatataatc gcatatgaat gaattggacc ggagatattc gcccagcttc
     1261 actgcctgtc tgtggccttt ttcagtgaga ggcgagtcga gaccgccgcc ctgaatcact
     1321 cctgcgagat tgaattcagt ttcaccgtgt cttacaagat ataaatgttt caccatagtt
     1381 ccagctttcc cggtgcagtt gcttccgggg gagtttctaa aattctgtca caggaaggac
     1441 atttttcaag gtgcctctgc attttga
//
LOCUS       DAOQGJ010001253         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67406_length_1464_cov_2.823279, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001253 DAOQGJ010000000
VERSION     DAOQGJ010001253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67406_length_1464_cov_2.823279"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="PK683_16905"
     CDS             complement(<1..1440)
                     /locus_tag="PK683_16905"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH10180.1"
                     /translation="MEYILRVWQVMTLFTITYSFTVTVKAVRMRLPNSRRLFWGMTAL
                     ILTAVFDILDSLILNTGLAFTKFAFLIFVIGVAGMLSNRFLELYEEVEDHRNNLEKKV
                     TERTKELQSTLQQVQILKEQQDGNYFLTTLIVNPLIAKKSSSESVKIDFLISQKKKFD
                     FKKKIHEIGGDICISESIVLRGKKYSVFVNGDAMGKSIQGAGGALVLGVVFHSLVSRT
                     RYFRTNKDIFPERWLKMAFQELQSVFESFDGSMLISVVMGLIDDENGAMYYMNAEHPW
                     TVLYRDGKASFIEDELTVHKLGMTIMESRFTVKTFQLLDNDVIIIGSDGRDDLKLFSG
                     DGNAINEDGDLFLKTVEETGAVLREIYEKTREIGEITDDFSLLRIHYNEIKWEYDAKQ
                     FDLHMKKAQLLYRKARRREAIDEFEKAYSHKRSEKNLLRIIARIFMKMKEYERAGNYF
                     AKYAEASPLDEKYIYMTAEAYRESGRTEDA"
BASE COUNT          390 a          361 c          263 g          450 t
ORIGIN      
        1 cgcatcctcg gttcttcctg actccctgta tgcttccgca gtcatataaa tgtacttttc
       61 atccagagga gaagcctccg catactttgc aaaataattg ccggcccttt catactcctt
      121 catcttcata aatattctgg caatgatcct gagaagattc ttctccgacc ttttatgtga
      181 atatgccttc tcaaactcgt caatcgcttc tcttcttctt gcctttctgt acagaagctg
      241 agcctttttc atatgcagat caaactgttt tgcatcatat tcccatttta tttcattata
      301 atggattcga agcaggctga aatcatcagt gatctcaccg atttctctgg tcttttcata
      361 aatttcccga agaactgctc cagtctcctc cactgttttc agaaaaagat ccccgtcttc
      421 attgattgca ttgccgtctc cgctgaaaag cttgagatca tcccgcccgt cggagccgat
      481 aatgatgaca tcattatcca gaagctgaaa cgtctttact gtaaaccggc tctccataat
      541 tgtcattccc agtttatgca cagtcagttc atcttcaatg aaggatgcct tcccgtctct
      601 gtacagaact gtccagggat gttctgcatt catatagtac attgctccgt tttcatcgtc
      661 gataagtccc atcacgactg aaatgagcat cgaaccgtca aaggactcga aaacactttg
      721 aagttcctga aaggccatct tcagccatct ttctggaaat atatccttgt tggttctgaa
      781 atacctggtt ctcgaaacca aggagtggaa aacaactccg agaacaaggg ctccgcctgc
      841 tccctgtatg gattttccca tcgcatcccc gttcacaaat acagaatact ttttccctcg
      901 aagaacaatg gattcagaaa tgcagatgtc tcctccaatc tcatgaattt ttttcttgaa
      961 atcaaatttc tttttctgac tgatgagaaa gtctatcttc acagattcac ttgaggattt
     1021 tttggcaatc agaggattga caatcagtgt ggtcagaaaa taattcccgt cctgctgctc
     1081 cttcagtatc tgcacctgct gaagagtgga ctgaagttcc tttgttcttt ctgtcacttt
     1141 tttttcgaga ttgtttcgat ggtcttccac ttcttcgtac agttccagaa acctgttgga
     1201 aagcattccg gctacgccta tgacgaatat aagaaaggca aatttggtga aagcaaggcc
     1261 ggtattcagt ataagcgaat ccaaaatatc gaataccgcc gttaaaatga gagcagtcat
     1321 tccccagaaa agccttctgg aattcgggag ccgcatcctg accgccttca cagttacagt
     1381 aaaagaatat gtaatcgtga acagagtcat tacctgccat accctcagta tatattccat
     1441 gataaatccg ggaacaaaga ggtt
//
LOCUS       DAOQGJ010001254         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67468_length_1463_cov_3.273438, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001254 DAOQGJ010000000
VERSION     DAOQGJ010001254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67468_length_1463_cov_3.273438"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..767
                     /locus_tag="PK683_16910"
     CDS             66..767
                     /locus_tag="PK683_16910"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HNH10181.1"
                     /translation="MKPIKKTDSDKPWNRRKKAESRKRETVQPSRIIYIFSEGTKTEV
                     IYFKSFPVLSAQVTAVGLGLSRTELIKESIKRKNSILQNSPKSEKKKYSFWCVYDQDI
                     ELTSLQVSNEERQKDFDDAFTLGRENELNLAYSNDSFELWFLLHYQYHDTVWTRKEYY
                     RKLSDLWNINYEKEGKKEEFCLSIYNKLSEKQNNAITNARKLFEEQSDLNPSLQNPCT
                     LVYRLVELLNKHIRK"
     gene            772..>1463
                     /locus_tag="PK683_16915"
     CDS             772..>1463
                     /locus_tag="PK683_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004476953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNH10182.1"
                     /translation="MAGNSSAIVNKIWNYCNVLRDDGVSYGDYLEQLTYILFLKMADE
                     KEALNEGQLNLIPKKYNWQSLRKESGEALEIKYNAILRELQKEKGMIGHIFLKAQNKI
                     QDPAKLSRLIQMIDAESWTEMDTDVKSEIYEGLLEKNASDVKGGAGQYFTARDLIWAI
                     VQCIRPEPGKTFADPASGTGGFFLGTYRYLKSLKLDRKQLQFLRLNTFFGWEIVPNAR
                     RMCLMNLFLNNIG"
BASE COUNT          511 a          246 c          324 g          382 t
ORIGIN      
        1 aaccacggga atccgaaatc ttgattatgc gggaaatttt gaagacttcc tgaaagagca
       61 ttccaatgaa gccaattaag aagacggatt cagataagcc ttggaatcgc agaaaaaagg
      121 cagagtccag aaaaagggaa actgttcagc catctcggat catttacatt ttttcagaag
      181 ggacaaagac tgaagttatc tattttaaat cattccctgt attgtctgct caggtgacgg
      241 cagtaggtct gggattgtcc agaacagaat taataaagga atcgattaaa agaaaaaaca
      301 gcatacttca gaacagccct aaaagtgaaa aaaagaaata cagtttctgg tgtgtctatg
      361 atcaggatat tgaactgact tctcttcagg tatccaatga agaaaggcaa aaggattttg
      421 atgatgcatt tactttaggc agggaaaacg aactgaattt ggcctactca aatgattctt
      481 ttgaactttg gtttctcctg cactaccaat atcatgatac tgtatggacg agaaaggaat
      541 attacagaaa actctcagac ctttggaata tcaattatga gaaagaagga aagaaagaag
      601 aattttgtct gtccatttat aataaactgt cagaaaagca gaataacgca ataaccaatg
      661 ccagaaaact gtttgaagaa caatctgatc ttaatccttc tctgcagaat ccctgtactt
      721 tagtatacag gctggtcgaa ttactcaata aacacataag gaaataaatc aatggctgga
      781 aattcatccg caatcgtaaa taaaatctgg aactactgca atgtgcttcg tgatgacgga
      841 gtcagttatg gggactatct ggaacagctg acatatattc tgtttttgaa aatggccgat
      901 gaaaaagagg cattaaatga aggtcagctg aatctgattc ctaaaaagta caactggcag
      961 agtcttcgta aggaatcggg tgaagcattg gaaattaaat acaatgccat tctgagagaa
     1021 ctgcagaaag aaaaaggaat gatcggacat atattcctca aggctcagaa taaaattcag
     1081 gatccggcta aactatcgag actgattcag atgattgatg cagaatcctg gactgagatg
     1141 gatacagatg tgaaaagtga aatctatgag ggccttctgg aaaaaaatgc atctgatgtg
     1201 aaaggaggag ccggtcagta ttttacggcc cgcgatctga tctgggcaat tgtgcagtgc
     1261 atacgtcctg aacctggaaa aacatttgct gatcctgcaa gcgggacagg gggatttttt
     1321 ctggggacat accgttatct gaaaagtctg aaactggaca ggaaacaact gcagtttctc
     1381 agactgaata cttttttcgg atgggagatt gttccgaatg caagaagaat gtgtctcatg
     1441 aatttatttc tgaacaatat agg
//
LOCUS       DAOQGJ010001255         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67504_length_1463_cov_2.355114, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001255 DAOQGJ010000000
VERSION     DAOQGJ010001255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67504_length_1463_cov_2.355114"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK683_16920"
     CDS             complement(<1..411)
                     /locus_tag="PK683_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH10183.1"
                     /translation="MKFIFLILFVYSEIMPNSLTDAAAAGDLDKVKTLIKKEKDLNEK
                     DEYGKTALMYAEEKRYSEIVKILIEAKADLNVRDNSGQTVLFRSLISDLGGGVDSETV
                     KMLIKSGANVNAKNHTGKTPLMHCTAERNLEMVST"
     gene            complement(570..1019)
                     /locus_tag="PK683_16925"
     CDS             complement(570..1019)
                     /locus_tag="PK683_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10184.1"
                     /translation="MKIIIALLLIFISYPVQSQQKINDSEAVWREVNKINSLNFKKKD
                     FQYCLADDPQDLNCYDEAMSIQAYGEFYYHKGRIRKVNYQVSMGYFGGEVTEYYSENG
                     QILHFYYNFNDVNSRRRYCEAGSVFFENGKSFQINCAPPTWPQDERS"
BASE COUNT          403 a          329 c          208 g          523 t
ORIGIN      
        1 agtactgacc atctccagat tcctttctgc ggtgcagtgc attagaggag ttttccccgt
       61 atggtttttt gcattcacat tcgctccgga tttgataagc atttttactg tttcagaatc
      121 cacacctcct cccaaatctg aaatcaagga tctgaacaga actgtctgcc ctgaattgtc
      181 ccttacattc agatcagcct ttgcttctat caatattttt acaatttctg aatacctctt
      241 ctcttctgca tacatgagag ctgtttttcc atattcatcc ttttcattca gatctttttc
      301 ctttttaata agagttttta ctttgtccag atcccctgcg gctgctgcat ctgtcagtga
      361 attgggcatt atctccgaat acacaaataa tattaaaaaa ataaacttca ttgaattctc
      421 ccttaattct gaaatccgaa aaatttgaat atactgcgaa tatttattat tacacgcttt
      481 ttaatcatct catttcttct tttcctttgg ctaatatttt cacctcgtat ccaggctgca
      541 gtaaatgaac tgattttgca gactgatctt cagcttctct catcctgcgg ccatgttgga
      601 ggggcacaat tgatctgaaa agattttcca ttttcaaaaa acacagaacc ggcttcgcag
      661 tatctcctcc tgctgtttac atcattaaaa ttatagtaga aatgcagtat ttgtccattt
      721 tcagaataat attcagtgac ttctcctccg aaatatccca tagaaacctg ataatttacc
      781 tttcgaatcc gacctttatg ataataaaac tcaccataag cttgtatact catagcttca
      841 tcatagcagt tcagatcctg aggatcgtca gcaaggcagt actgaaaatc cttctttttg
      901 aaattcagtg aattgatttt attcacttcc ctccagactg cttccgaatc attgattttc
      961 tgctgagact gcacaggata cgaaataaat attaacagaa gagctattat tattttcata
     1021 aagtatttgt tcatttcaga catcagtctg tctgaacaaa atgaggcaaa atccgctttc
     1081 acttgcatcc aaaccctgtc tcgttttgca gagacatcgt acggttagga ttaattgaac
     1141 ctttgcagct gacagttatc attttcaatt ccttgcttgg gctcgcccat tgccttctag
     1201 attcgggcca atcagtcgat atgtccgtcc aataagttat cttgaatttt atttctcttt
     1261 tttcctcatc tatgaacaaa tcttccaatt ttacagaagg agtataatca acatcgtagt
     1321 tactcgacat caaatctaaa aaaaagaaat attcacctca tcgagagctt cctcacttgt
     1381 ccttcgtcat tcctctcctt cttctatcaa atgaattttt ttctcattct tgataatcat
     1441 ttgaggcact ttgttaaatt ctt
//
LOCUS       DAOQGJ010001256         1462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67614_length_1462_cov_2.269367, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001256 DAOQGJ010000000
VERSION     DAOQGJ010001256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67614_length_1462_cov_2.269367"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1306
                     /locus_tag="PK683_16930"
     CDS             344..1306
                     /locus_tag="PK683_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNH10185.1"
                     /translation="MLAFRYKLINIVSMKESDIILSLYPENSAPLDDCFLWEKKNTLI
                     STDTICEGTHFRLDWSSPEDIAVKLLEVNVSDLISSGGQPETAFLNLGISQRQDSDWL
                     KRFSSELKYRLKKRGVILAGGDTYFSPVLNLTMTVLGERIFHSPRTGANAGENLYLTG
                     PLGYSNAGYIHLSEKRKLPEWVLEKAIQKHLRPESRMDIFSLLRNINISAMMDMTDGL
                     IQDSEKFAAASGKSLLIYADRIPEFGFLSKCLNTDEILSSGEELELLFSSRETLTDFA
                     CIGKVTDVNSAGGKPEVRFVLNGQEFIPEKKGFFHFQGESIGQN"
BASE COUNT          429 a          292 c          322 g          419 t
ORIGIN      
        1 gaacatgcat tgtatccggg catctttaca gatatctgaa tttaatttct ctcgaccgaa
       61 aggatcattt tctttacata aatgaaaaag ggaaactgtg ttctgtgcaa caggaagaat
      121 cctgcagata gatgctggaa gtttctgtac aatggatcgt ttgctttact tcctgaaacg
      181 aagaaatact tgtgatttat atatataaat tttatatata ggtaaatgaa tacttttgag
      241 gcaatcagct gaatgccgat atgcgacatc attttgcatc atattttaat ttgtatataa
      301 attgctttgc cgttctgtct tgcctgaaac cggaaaactg tttttgcttg ctttccgtta
      361 taaactcatt aatatcgtct ccatgaaaga gtctgatatc attctgtcac tgtatcctga
      421 aaacagtgct cctctcgacg actgcttttt atgggagaag aaaaatacac tgatttccac
      481 ggatacaatc tgtgagggaa cacatttccg tctggactgg agttcgccgg aggacatcgc
      541 agtcaaactt ctggaagtca atgtttccga tctcatttcc tccggaggac agcctgagac
      601 tgcttttctc aatctgggaa tttcccaaag acaggactcg gactggctga aaagattcag
      661 ttctgaactc aaatacaggc ttaaaaaaag gggagtgatt ttggcaggag gggacactta
      721 cttttcaccg gttctgaatc tgacaatgac agttctaggt gaaagaatat tccattctcc
      781 ccgcacaggt gcgaatgcag gagagaatct gtatctcaca ggcccgctcg gatattccaa
      841 tgcgggatat attcatcttt cggagaagag aaaactcccg gaatgggttc ttgaaaaagc
      901 aatccagaaa catctccggc ctgagtccag aatggatatt ttttcattat taaggaatat
      961 aaacatatca gcaatgatgg acatgacaga cggtctgatc caggactctg aaaagtttgc
     1021 tgcagcttcc ggaaaatccc tgctgattta cgcagacaga attccggaat tcggctttct
     1081 ctcgaaatgc ctgaatacag acgaaatttt aagttcaggg gaggaactcg aacttctttt
     1141 ctcctcaagg gaaacattga cagacttcgc ctgcattggc aaagtgactg acgtgaattc
     1201 agccggagga aaaccggaag tcagatttgt attgaacggt caggaattca ttcctgaaaa
     1261 aaaaggattt tttcattttc agggagaatc gattggacag aattgaattt aaacttcgga
     1321 accgtctgga ggttcagcct cccggatacc gttccaacag tgcaatggac gggtacagga
     1381 ttccagtatc ctatatcgat tttatcgtga acggaaagtc tcttgcagat cttctggaag
     1441 cagacaggcg ggatctggtc ag
//
LOCUS       DAOQGJ010001257         1461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67683_length_1461_cov_2.460882, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001257 DAOQGJ010000000
VERSION     DAOQGJ010001257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67683_length_1461_cov_2.460882"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..465
                     /locus_tag="PK683_16935"
     CDS             88..465
                     /locus_tag="PK683_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10186.1"
                     /translation="MKQFLIFVLLSILSFSNLYAHGQEYLIVITYAFWVISPLLYLII
                     TFVFNWRLVKKYGRNPAIELHLWKLNLFTFGLLVLGAVISTILYVKHIISETEVAWLL
                     FLIPAGIYGSIAFLTLKKSSPKG"
     gene            750..>1461
                     /locus_tag="PK683_16940"
     CDS             750..>1461
                     /locus_tag="PK683_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH10187.1"
                     /translation="MLGIRFLKVKPTEHVILFKKGKIQKEGAGQSFFYYAPSSSVVIV
                     PAASSDVPFIFKETTVDYQEINIQGQFAFKVLEHSKLASVLDFSVNADGIQIGDGNEK
                     LTTRLSNIVQVTVREKLSGLDLRSALKAGTEFTAHVKERVKDNDALKGLGVEISDFAI
                     LKISPTPDMSRALEAGARETLLKEADEAIYQRRNFAVEQERKIKENELQTQIAIEEKN
                     KKIREEQMNIEIAVQEKQR"
BASE COUNT          461 a          303 c          300 g          397 t
ORIGIN      
        1 cagagaatat tccgctccgg acatttccca ataaactttt tcagattttc atttgacaga
       61 aaattcaggc ggtgcagaaa catcgttatg aaacaatttc taatattcgt tctgttaagt
      121 attctctctt tttcgaatct ctatgctcac gggcaggaat atctgatcgt cattacatat
      181 gcattctggg tcatcagtcc actgctgtat ctcatcatta cttttgtatt caactggaga
      241 cttgtcaaaa aatacggaag aaatcctgca atagagctgc atctgtggaa gctgaacctg
      301 tttaccttcg gccttcttgt tctgggtgca gtcataagca caattttata tgttaaacat
      361 attatcagtg aaacagaggt tgcatggctt ctttttctca ttcctgcagg gatctatgga
      421 tcaatagcat tcctgactct gaaaaagtct tcaccgaaag gatgacggac aggcggactg
      481 tgtaaaggca taaactttct ttctgcagat ggattcagta aagaaaatca ctgtcttttc
      541 ctgacatgct tttacatacc ccgcccagca ttatcctgaa atcataagca gcaagactga
      601 aatccattgc acacctaaga cattcaccaa acttatagaa tggaaatcca tcttctgaat
      661 cagaaggctt tttccggcat ccctgaactc tgactttaaa cgaataaaaa aagcttgcag
      721 ttttttttca ttgagatatt ctgtggcgca tgttaggaat ccgatttctg aaagttaaac
      781 ccactgagca cgtcatcctt ttcaaaaaag gaaagatcca aaaagaagga gccggtcaga
      841 gtttcttcta ttatgcccct tcttcgagtg tagtcatagt ccctgccgca agctccgatg
      901 ttccattcat attcaaggaa accacagtcg attaccagga aatcaatatt cagggacagt
      961 ttgcgtttaa agttctggaa cattcgaagc tggcatcagt tctggatttc tcagtcaatg
     1021 ctgacgggat tcagatcggc gacggaaatg aaaaactcac tacacggctt tccaatatag
     1081 tgcaggtgac tgtacgggaa aaactttcgg ggctggatct tcgttctgct cttaaggcag
     1141 gaacagaatt cactgctcat gtgaaagaaa gagtcaagga caatgatgca ctgaaaggac
     1201 tcggtgtgga aatctcagac tttgcaatac tcaaaatcag tcctacacct gatatgtcga
     1261 gggctctgga agcaggtgca agagaaacgc ttctcaagga ggcggatgaa gctatttacc
     1321 agagaagaaa ctttgcagtg gaacaggaaa gaaagatcaa ggaaaatgaa ctccagacac
     1381 agattgcaat tgaagaaaaa aacaaaaaga tccgcgaaga acagatgaat atagaaattg
     1441 cagtccagga aaaacagaga c
//
LOCUS       DAOQGJ010001258         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67782_length_1460_cov_2.151601, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001258 DAOQGJ010000000
VERSION     DAOQGJ010001258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67782_length_1460_cov_2.151601"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PK683_16945"
     CDS             <1..1052
                     /locus_tag="PK683_16945"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNH10188.1"
                     /translation="LARMVAARLRDLQRPIRWFSIPNLWRYEAPGHGRLREHWQLNVD
                     LFGVDSSRAELEIILLAAEILSSFGADSSMFKIQFSHRKILNDFLKKVLVLNEEQEYA
                     MAKLLDKRNKITEKEFKDSAEKLLDDPGRQYALIEKYLNCDINSLKQFSEIGRETVEF
                     LEQFESGCKEAGIADVLKFDPSIIRGFDYYTGFIFEIFDMDPENRRSLYGGGRYDNLV
                     GLFSNEQISGIGFGLGDVTLLNFLKSHGLVPDLRERNSVYIPLLSDSMFTDLVKISSQ
                     LRKAGISAELSLDAGKFGKQISAAEKKGYSHILIYGEDEKKEESLVLKNLNSGEQKKL
                     KLSEMNSVLQKELNS"
     gene            1049..>1460
                     /locus_tag="PK683_16950"
     CDS             1049..>1460
                     /locus_tag="PK683_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNH10189.1"
                     /translation="MISLILKIDTEIALFFHRKFADSNFREFVVKINRGEISIPIAFV
                     LLFLTVGWQSQYIYSFFYIVIFCFLNDRFVLFIKKKISRRRPSLKLLGKPDNHPDLNH
                     SFPSAHAANSLAAVVLLNQIWGASPLFAFISFLAG"
BASE COUNT          413 a          299 c          336 g          412 t
ORIGIN      
        1 cgcttgccag aatggttgca gcaagactca gggatctgca gagacccatc agatggtttt
       61 ccattcccaa tctctggaga tatgaagctc cgggtcacgg gagactcaga gagcactggc
      121 agctgaatgt ggatctgttc ggtgtagatt cctccagggc agaacttgag atcatccttc
      181 tggctgcaga gattctttct tccttcggtg ctgattcttc aatgtttaag attcagtttt
      241 ctcacaggaa aatcctgaat gactttctga agaaggtgct tgtactgaat gaagagcagg
      301 aatatgcaat ggccaagctg ctcgataaaa gaaacaagat tacggaaaaa gagttcaagg
      361 attcagcgga aaaacttctg gacgatccag gcaggcagta tgcgctcatt gaaaaatatc
      421 tcaactgtga tataaattca ttaaagcagt tcagcgaaat cggcagagaa accgtggagt
      481 ttctcgaaca gtttgaatca gggtgcaaag aagcaggaat tgcggacgtt ctgaaatttg
      541 atccttccat catcaggggt tttgactatt atacaggctt tatcttcgaa attttcgata
      601 tggaccctga aaacagaaga tcactgtacg ggggcgggag atacgacaat ctcgtgggac
      661 tgttttcaaa tgaacagatt tccggaatag gcttcggact gggagacgtc actctgctga
      721 acttcctgaa atctcacggt ctggttcctg atctccggga aagaaacagt gtctatattc
      781 ctcttctctc agacagtatg ttcactgacc ttgttaaaat ttcttcacag ctcagaaagg
      841 cgggaatttc tgccgagctc agtctggatg caggcaagtt cggaaagcag atctctgctg
      901 ctgaaaagaa aggctacagc catattctca tatacggtga ggacgaaaag aaagaggaaa
      961 gtcttgtttt gaaaaatctg aattcaggag aacagaaaaa actgaagctt tctgaaatga
     1021 attctgtgct gcagaaagag ctgaacagtt gatctctctc atcctgaaga tcgatactga
     1081 aatcgctctt ttttttcaca gaaagtttgc agattcaaat ttcagagagt tcgtagtaaa
     1141 gatcaacaga ggcgagattt ccattccgat cgcctttgtt ctgctttttc tgacagtcgg
     1201 ctggcagtcc cagtacatat acagcttctt ttatattgtc atattctgct ttctgaatga
     1261 caggtttgtt ctttttataa agaaaaaaat atccagaaga agaccttctt tgaaactgct
     1321 cggcaagccg gacaatcatc ctgatctgaa tcattctttt ccgtcagcac atgcggcaaa
     1381 ttcacttgcg gcagtcgttc tgctgaatca gatctgggga gcgagtcctc tctttgcatt
     1441 tatttcgttt cttgcaggtc
//
LOCUS       DAOQGJ010001259         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67862_length_1459_cov_2.240741, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001259 DAOQGJ010000000
VERSION     DAOQGJ010001259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67862_length_1459_cov_2.240741"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PK683_16955"
     CDS             complement(<1..422)
                     /locus_tag="PK683_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10190.1"
                     /translation="MRISRNYLSVQDQLLRGIEQLALGWFFIHKTENEKKKVPMDLII
                     SILEEAYSEQDGSSEEETDYLSRRYKNYREKLEEKPYQVKTDFQKKIIQNFKGNEDGI
                     RNLAKLYLENYAEDYSDYILENYLKIQKQNGLLEYSLFA"
     gene            complement(439..1356)
                     /locus_tag="PK683_16960"
     CDS             complement(439..1356)
                     /locus_tag="PK683_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10191.1"
                     /translation="MLKPKSSINTTKKASEVCGRILKLNSKGNFSFSNSHIYGFRTTV
                     IPKINGRDNEEFKQLERKIAFRGIIKKVDDLRIAELRGLVSKFKSRYEIAEDFKEIQI
                     NNSQKMSKQRVSSYSSLVRFFLTYSAYEGFCDLFFEKDTDLRAKQELYKLLESSTKQV
                     NKLQKILDNDKKNIISEYLISEMESDDIAFRLMFFFNINLQAIQKINQQKTTEYKNKT
                     DQDKKNIPLPNEGDILLIPRAFRHKIAHGHLTASLTIYDAPKKEKTIGYGTMRKLFDA
                     MSDLLFSIMDNKFNEIDQQIANQQTPTGP"
BASE COUNT          399 a          261 c          234 g          565 t
ORIGIN      
        1 gcaaagagtg aatattccag aagaccgttc tgcttttgaa tcttcaagta gttttccaga
       61 atataatcgc tgtaatcttc tgcataattt tccagataca gttttgcaag atttcgaata
      121 ccgtcttcat tgcctttaaa attctggatg atttttttct ggaaatcagt tttcacctga
      181 taaggttttt cttccagttt ctcccgataa ttcttatatc tgcgagacag ataatcagtt
      241 tcttcttcag aactgccgtc ctgttcactg taagcctctt cgagaatact gatgatcaag
      301 tccattggga ctttcttttt ttcattttcc gttttatgaa taaaaaacca gccgagagcc
      361 aattgttcta tccctcttaa aagctgatcc tgaactgaaa gataatttct gctgatgcgc
      421 atctgtgaat caaaaaaatt aaggaccagt tggagtctgt tgatttgcaa tctgctgatc
      481 tatctcatta aatttattgt ccattatgct gaacaagaga tcagacatcg catcaaaaag
      541 ttttctcata gtgccatacc ctatggtttt ttcctttttc ggtgcatcgt atattgtcag
      601 actggcagtc aaatgtccat gtgcgatttt atgccgaaac gctcttggaa ttaggagaat
      661 atctccctca tttggtaatg ggatattttt tttatcttgg tcagttttgt ttttatattc
      721 ggtggttttt tgttggttaa ttttttgtat tgcctgtaga tttatgttga aaaaaaacat
      781 cagccgaaat gcaatatcat cagattccat ttcagaaatc agatattcag atattatatt
      841 tttcttatca ttgtctagaa ttttttggag tttattaacc tgcttagtgc tagattcgag
      901 taatttatat aattcttgtt tggctctcag atctgtatct ttttcaaaaa acaaatcaca
      961 gaatccttca taagcagaat atgtcaaaaa gaatctaact aaagaactgt atgatgatac
     1021 tctctgctta gacatctttt gggagttatt gatttggatt tctttaaagt cttccgcaat
     1081 ttcatacctg gacttaaatt tgctgactag tcctcgcaat tcagctattc taagatcgtc
     1141 tacctttttg attatacctc taaatgctat ctttctttcc aattgtttga attcctcatt
     1201 atctctgccg ttaattttcg gaataactgt tgttctgaat ccgtatatgt gcgaattgct
     1261 aaaagagaaa ttccctttac tgttcagttt taaaattctt ccgcagactt cacttgcttt
     1321 ttttgtggta tttatagaac tcttgggttt cagcacaata ttctcctgat ttccagcaga
     1381 tttgtattat ttcataaaaa tttcaagcag tattttggaa aaagaaatgt gtttttgatt
     1441 ttcccaaaaa tagagcctg
//
LOCUS       DAOQGJ010001260         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67865_length_1459_cov_2.121083, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001260 DAOQGJ010000000
VERSION     DAOQGJ010001260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67865_length_1459_cov_2.121083"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..>1459
                     /locus_tag="PK683_16965"
     CDS             265..>1459
                     /locus_tag="PK683_16965"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNH10192.1"
                     /translation="MKKILSLITAVTLSFSFCAHKSRTKLFQNSLRSDVDIFLIPKKF
                     NIMESEWVETRDDSSFLKSDLIRHLKNYGRNLEMLKNDPSVKYREKDDIITMNVAKTS
                     HSTFYEYDTNIPVIFYGPRWIQKGIYQDLIHQQNIVPTLAKITGVRNPNGVEVPALSH
                     ILKIQSPREKPEIIVTVVIDQGGQQVYKAHPGVPKNIEKIKESSAYFPNAHVGHLDAH
                     TAVGHMAIGTGSYPSKSTVVGNTFFHIRGGIVHSNEIYALNETEVNPDELHTESFADV
                     LDSENGNGPEVISQCYALRASIGMAGHGAFRLKNSSVNPDKDFVYWLDKKDGKWTTDT
                     RYYQLPAAAKDFLPYETHLKNYPEGWKGHTGTRIEDLKKHWSTVMAAPAEARLEGELF
                     RKVIQQ"
BASE COUNT          493 a          284 c          328 g          354 t
ORIGIN      
        1 tatggccaat ttggagaaat gcatggacga aatgatgatc aacggtaaga tgatggaata
       61 aatgatgaag cagaacgata ctggagttga tactacagcc taaaacatgc ttgatgtatt
      121 aaagggcgaa atcgctatgt aagcataaaa taaaataaac gaggcagcga tgatccagca
      181 acaagagatg caatttcaat aagacttaaa aaaattatga acaacttaat taattaacta
      241 taaagaatac attgtcagaa cttaatgaaa aaaattttat cactcatcac ggcagttact
      301 ctcagtttct ccttctgtgc acacaaaagc aggacaaaac tttttcagaa ttcgctcaga
      361 agcgatgtcg atatatttct tatcccaaaa aaatttaata taatggaatc cgagtgggtt
      421 gagacaaggg atgattccag ctttctcaag tccgatctga tccgtcatct gaagaattac
      481 ggaaggaatc tggaaatgct gaaaaatgat ccttcagtaa aatacagaga aaaagatgat
      541 atcattacaa tgaatgtggc aaagacttct cattctacat tttatgaata tgacacaaat
      601 atccctgtca tattttacgg tccccgctgg attcagaaag ggatatacca ggatctgatt
      661 catcagcaga atattgtacc tacactggca aaaattaccg gtgtcagaaa tccgaacgga
      721 gtggaggttc ctgcacttag ccatatcctg aaaattcaga gtccccggga aaagccggaa
      781 atcattgtga ctgttgttat tgaccaggga ggacagcagg tgtacaaggc tcatccagga
      841 gtgcctaaaa atatagaaaa gatcaaggaa tcctcagcct attttccgaa tgctcacgtc
      901 ggtcatctcg atgcacatac tgcagtcggt catatggcaa tcggcacagg ttcctatcct
      961 tcaaaaagta cagttgtagg gaacacattc tttcacataa gaggcggtat cgttcattca
     1021 aacgagattt atgccttgaa tgaaacagaa gtaaacccgg atgagctcca cacggaaagt
     1081 ttcgcggatg ttctggacag tgaaaatgga aacgggcctg aggtgatcag tcagtgttac
     1141 gctctcaggg cttccatagg aatggcaggt catggtgcat tccgtctgaa gaacagctca
     1201 gtcaatccgg acaaggattt tgtctactgg ctcgacaaga aggacgggaa atggaccacg
     1261 gatactcggt actatcagct tccggcagcg gcaaaggatt tccttcctta tgaaactcat
     1321 ctgaaaaatt atccggaagg ctggaaagga catacgggaa ccaggattga ggatctgaaa
     1381 aaacactgga gcactgtaat ggcagcaccg gcagaagcga gactggaagg cgaacttttc
     1441 agaaaggtca ttcagcagg
//
LOCUS       DAOQGJ010001261         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67930_length_1458_cov_2.151818, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001261 DAOQGJ010000000
VERSION     DAOQGJ010001261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67930_length_1458_cov_2.151818"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            587..1453
                     /locus_tag="PK683_16970"
     CDS             587..1453
                     /locus_tag="PK683_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10193.1"
                     /translation="MRKSISLIFLLLCTIQPVFLQEKNGERKKAQTDWTNSKVSLTVM
                     ESLPRVIIDTKDIEYGGDHTAYNVSEARNKALSKAKERSSLQIVKAVENIRLNDEYLI
                     LEKLNDDPSGKFRERFNQFFFEEKSELKVKFLRDKVAVESIIPFRGKSGLLNYLDVEY
                     EREKFPDFRDSRMKEAYTSLIVDARHLDFVPSVFPTIVTEKGIEIYSKDMVPAKYGID
                     EGIVQYHKDYKQAFLDSRAGSKPFFVLALTVAGKNRTSVSIPTDEAKKLFSSEETKAS
                     LKKCNVIILMQD"
BASE COUNT          459 a          303 c          287 g          409 t
ORIGIN      
        1 tcctcagatt ctcaatttcc ctgcagatat tcgccacact gtcagactcg ccagtctcat
       61 aatttgcttc cagaaaattt tccaggtttt caagccttgt cttcagtgtc ttctgatcaa
      121 cacaatcctt ttttcgccag aacatccact cctcctttcg tttggcagac cgaacagcct
      181 gagactgaaa tcagaccgtc attcaaatgg actgcaatca aatcctgcac gcctttgcat
      241 tgagctgaac cgcagtaagg cagtctcagg ctggccaaca ggatgaacat accattagtt
      301 agacataaaa taacatatgg actaattaaa gttaattaca atctcagtat ttcaatcgga
      361 cttttcaata tgtaggttaa gaggaaaata tgcccaattt cctcattgga cacctttttg
      421 actttttatg cattatttaa tgaatcttgt tgacatctgg aaataaacag cccgaatctg
      481 aaattctagc ggtttattaa taatttctgt ctcaagcaga ttcaagtatc ccctaggaga
      541 aatcatctga tttgagaaaa aacccgtatc tgacgatgtt taagatatga ggaaatctat
      601 ctcactgatt tttctattgc tctgcactat tcagcctgtt tttttacagg aaaaaaacgg
      661 ggaaagaaag aaagcccaga ctgactggac aaattccaaa gtttccctga ctgtaatgga
      721 gtccctcccc cgagtcatca ttgataccaa agacattgag tacggcggag accatactgc
      781 atacaatgtc tccgaggcta gaaacaaagc cttgagcaag gcaaaggaaa ggagttctct
      841 tcagatagtc aaggctgtgg aaaacatccg tctgaacgat gagtatctga ttctggaaaa
      901 actcaatgat gatccttctg gaaagttcag agaaagattc aatcagttct tttttgaaga
      961 aaagtctgaa cttaaggtaa aatttctgag agacaaggtt gcagtggagt ccattattcc
     1021 gtttagaggg aaatcaggtc ttttgaatta tctggatgtg gaatacgaaa gggaaaaatt
     1081 ccccgatttc agagattcca gaatgaaaga agcctatacc agtctgattg tggatgcccg
     1141 tcatctggac tttgtaccct ccgtttttcc tacaattgta accgaaaagg gcattgagat
     1201 ttattcaaaa gatatggtac ccgcaaaata tggaattgat gagggaatcg tccagtatca
     1261 caaagactat aagcaggcct ttctggactc cagggcgggc tccaaaccat tctttgtgct
     1321 tgcactgacg gttgcaggaa aaaacaggac gagtgtatcc atacccacag atgaagcaaa
     1381 aaaacttttt tcctcggaag aaaccaaggc aagtctgaaa aaatgcaatg tgattatttt
     1441 aatgcaagac tagccttt
//
LOCUS       DAOQGJ010001262         1457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_67960_length_1457_cov_3.570613, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001262 DAOQGJ010000000
VERSION     DAOQGJ010001262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67960_length_1457_cov_3.570613"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..692
                     /locus_tag="PK683_16975"
     CDS             381..692
                     /locus_tag="PK683_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10194.1"
                     /translation="MNTVWNHTKEGRQYWVRLKRDSMNLEFISDPVSELAGGGYSFDL
                     PEDLSADTEELKELGKVFTKQDLQEMQAFSRALKKLKRSDIKNRTERFYQIYYEELEN
                     L"
     gene            complement(1066..>1457)
                     /locus_tag="PK683_16980"
     CDS             complement(1066..>1457)
                     /locus_tag="PK683_16980"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNH10195.1"
                     /translation="TLSNFINLKELDLSSCSEISEISPIKGLKKIRKLNLASCGKIKD
                     ISAIKSMKNITELNLSFCEKITDLSPLAGLKNLRILNLESCTGLKDISVLKNLKNLAE
                     LNISGCTGISDFSAVTHIPDLILNDQE"
BASE COUNT          452 a          284 c          319 g          402 t
ORIGIN      
        1 cactcagcat gaaattacgt ctcttatcaa tgtatttccg gggaaatccg ctgtatttgc
       61 atttggtcca tttttcattg aatgaaactg gattttcttc accggagaat atcatcgaaa
      121 agactgataa tattcgggat gaagattgca gaggcggcag ccgattttca gggccttcaa
      181 gccgtcggca tgtcccaaaa acgccccgcc ttgcagccac ctgtgcagct gtaggcggcc
      241 tacagtgtaa ggagaattac taaaatatat tatactgatt tcatattctg tcgacagaaa
      301 ggagtgacaa tattggattc tgtaagcaaa tatacatacg gataaagact gaatactgga
      361 gtgctaagaa cctggaaaca atgaacacag tatggaatca tacaaaagaa ggcagacagt
      421 actgggtgag gctgaaaagg gattcaatga atctcgaatt catcagtgat cctgtttcag
      481 agctcgcggg aggagggtat tcatttgatc tccccgaaga tctgtctgca gatacagaag
      541 aacttaaaga acttggaaaa gtttttacaa aacaggatct tcaggaaatg caggcgtttt
      601 ctcgagcact gaaaaaactg aagcgttccg atattaaaaa ccggacagag agattctatc
      661 agatttatta tgaagagctg gaaaatctgt gagactgcac ctcgaaagca gtcaatgcaa
      721 attgactgtt ggaagtcctg aataaacagc atactgtcaa tgtcgatgaa gagcccgccg
      781 aggatgaatt cgtagagtag aaatgattct gaattactta tgttacgcaa atacgatatg
      841 ctgtctagac gctgtaattt tcagaaagta ttagaaaaat ctagcagttt aaaagtttgc
      901 gatgaatcag taaaaacagc atcttccagg cttgagggag ttgagccagc ttctaaatgt
      961 gcagcagctt ccaaatgtgc agcagcttcc aaatgtgcga gccctcaagc cgccggcagg
     1021 cgctctgccc aaaacgcccc acctgcgaag atgagtcaat aatttctatt cctgatcatt
     1081 taaaatcagg tcaggaatat gcgtcactgc actgaagtca ctgattcctg tacagccgga
     1141 aatattcagt tcagcaaggt ttttcagatt cttcaataca gagatatctt tcagtcctgt
     1201 gcaggattcc aaattaagta ttctcagatt cttcaggcca gcgagcggag aaagatctgt
     1261 gattttttca cagaaggaaa gattcagttc tgtgatattt ttcatgcttt ttatagctga
     1321 aatatctttt atttttccgc agcttgccag attcagtttc cggatttttt tcagaccttt
     1381 aataggcgag atttccgaaa tctctgaaca gctagaaaga tccagttcct tcagatttat
     1441 aaagttggaa agggtac
//
LOCUS       DAOQGJ010001263         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68049_length_1456_cov_3.398287, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001263 DAOQGJ010000000
VERSION     DAOQGJ010001263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68049_length_1456_cov_3.398287"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PK683_16985"
     CDS             complement(<1..961)
                     /locus_tag="PK683_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10196.1"
                     /translation="MNKLSFSILLLAVSFGAGCKSGNEELAVFEGGKIIRQDIRDYYA
                     LQNIQINETTGSIKNQTSVLENLAIQSLVQKENSGKKVEETADYKNLREISERQLTVN
                     LYIRNFLNQVKEDRDMEMAEIQFIFMRGGSEKSEAVFKEISALSDKKAVNEFIMKNTE
                     EEGRKALAGYLEPQCINCLASQLTELVFKEGIEKKDGKFYKKEFEGNSYIYRITEVKK
                     VKAKKLEAYLKGKFKDFREMAIEYQNAAQNENQKKSAEYYAEDDPKLSEKAKMTANHY
                     TKKFEEKIWPDEFEKLLQDSGYLIAEVAKQSPDILSKEKPETVL"
     gene            complement(963..1241)
                     /locus_tag="PK683_16990"
     CDS             complement(963..1241)
                     /locus_tag="PK683_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10197.1"
                     /translation="MKLFELQSLFESIERDIDELKEARKKIPDGRDYSASILESIEKE
                     IRKLEEKKAKILNMEIQLPENIFPVKEEKKLPEPVIKTAEKPKNIRRY"
BASE COUNT          363 a          286 c          253 g          554 t
ORIGIN      
        1 gcagaacagt ttccggtttt tccttggaaa gaatatcggg actctgtttt gcaacttctg
       61 caatcagata tccggaatcc tgcagaagtt tttcaaactc atcgggccag atcttttctt
      121 caaatttttt ggtatagtgg tttgcagtca tcttcgcttt ttcagaaagc tttggatcgt
      181 cctctgcata atattccgca gattttttct gattttcatt ctgagcagca ttctgatatt
      241 cgattgccat ttcacgaaag tccttgaatt ttcctttcag atacgcttcc agtttttttg
      301 ccttcacttt tttgacttca gtaattctgt agatgtaact gtttccttca aattcttttt
      361 tatagaactt tccgtctttt ttttcaatcc cttccttgaa cacaagttca gtcagctgag
      421 aggcaagaca gttgatgcac tgaggttcga gatatcctgc aagagccttt ctgccttctt
      481 cttcagtatt tttcatgata aattcattaa cagccttctt atctgaaaga gcagaaattt
      541 ccttgaagac agcttctgat ttttcacttc cgcctctcat aaaaataaac tgtatctctg
      601 ccatttccat atccctgtct tccttgacct ggttcaggaa atttctgatg taaagattta
      661 cagtcagctg tctttctgaa atttctctca ggtttttata atcggcagtc tcttccactt
      721 ttttgcctga gttttctttc tgaacaaggg actgaattgc aagattttcc agaacggaag
      781 tctgattttt aatcgatccg gtggtttcat ttatctgaat attctgcagt gcataataat
      841 cacggatatc ctgacggatg atttttccgc cttcgaaaac ggcaagttct tcgttgcctg
      901 atttgcatcc tgctccgaat gaaactgcca ggagaagaat ggagaatgaa agtttattca
      961 tgtcagtacc ttcttatgtt ttttggtttt tctgctgttt tgatgaccgg ttccggaagt
     1021 tttttttctt ctttgactgg aaatatattt tccggaagct gtatttccat attcagtatt
     1081 tttgctttct tttcttcgag ttttcgaatt tctttttcga tggattcaag aatgcttgca
     1141 ctgtagtctc ttccatccgg aattttcttt ctggcttcct tgagttcatc aatatcccgt
     1201 tcgattgatt caaacagaga ctgcagttca aacagtttca tttatttttt accggctgaa
     1261 aacatctgat agtggaaaga aaaaaaaaat cagtcattca gtcaaaaatt aaacagtctg
     1321 atttttacaa aaacagtttt tttattatca ggatgccgag acgaaaagct aggaagaaga
     1381 aggagaattt accggtgtgt tcttaaaatt ttattagtac gtttttatac catattgcgc
     1441 cttgtagtat caaatg
//
LOCUS       DAOQGJ010001264         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68167_length_1455_cov_2.491429, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001264 DAOQGJ010000000
VERSION     DAOQGJ010001264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68167_length_1455_cov_2.491429"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..547
                     /locus_tag="PK683_16995"
     CDS             95..547
                     /locus_tag="PK683_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10198.1"
                     /translation="MTRLYLLFVFIFFLSCSKTQSENNLKLVSNVLEENKKVYELFLA
                     QGNAKPDLTGLKEAVRKASISADKDSLKKSFSEMLAVLESANQSETAGSISVFSEKLY
                     STLKAEEISSGYRKFYCPMEKKFWLAKGDDVKNPYAPEMRECGELVQE"
     gene            549..>1455
                     /locus_tag="PK683_17000"
     CDS             549..>1455
                     /locus_tag="PK683_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10199.1"
                     /translation="MNQSNSKAQGKCFLDTGLYESCIGNCKSVLSYNTWLGNLESLCI
                     ARKKLDEFMEGSNTFSVAISAFTQCQPAILDIFASKSEAALDLIRYFFSISEPEETRQ
                     IAEKMSNEILYRILAKDYENFVNIQKMVDRRKAAVNYFAVKSSAFWKLMSQERLCSII
                     VFLVRDVHEYKLAAQFLMILSPEVISQFTKFTNLSEDDERNLFLALEDNIYNIPLVSP
                     KIYGHMLELFKDEFEIFFVLETMGPLVKRRAEIEDISMSFLRYHKRTGQKLSIQWIYS
                     ELFGLEYELVVEILNQLMENEFITLS"
BASE COUNT          468 a          253 c          304 g          430 t
ORIGIN      
        1 aagtgacaaa ctgaatagaa aataagcacg gtattattcg gattacactg tttatttata
       61 ctgacctatc gccgaaaata agtaagaggg taaaatgact agactgtact tattatttgt
      121 attcattttt tttctttcct gctctaagac gcagtctgaa aacaatttaa aactggtttc
      181 caatgttctg gaggaaaata aaaaagtgta tgaacttttt ctggctcaag gaaatgcgaa
      241 accagatttg accggtttga aagaggctgt aagaaaagca tccatatccg cagacaagga
      301 ttcattaaaa aaatctttct ctgaaatgct tgcggttctt gaatcagcaa atcagtctga
      361 aactgcagga agcatatccg ttttttctga aaagctgtat tcaacgctga aagcggaaga
      421 gatcagttct ggatacagga aattttattg tccaatggaa aaaaaattct ggcttgcaaa
      481 aggtgatgat gtaaaaaatc cctacgctcc tgaaatgaga gaatgcgggg agctggtgca
      541 ggaataattt gaatcagtca aattctaaag ctcagggaaa atgttttctg gatacaggtc
      601 tgtatgaatc ctgcatagga aactgtaaat ctgtactgag ttacaatacc tggcttggga
      661 atttggagag tctctgcatt gccagaaaaa aactggatga atttatggaa ggttccaata
      721 cattcagtgt ggcaatttct gcatttactc agtgtcagcc cgctatactc gatatttttg
      781 cttcaaaatc ggaagcagcc ctggatctga tcagatactt tttcagcata tccgaacccg
      841 aagaaacaag acagattgca gaaaaaatgt ccaatgaaat cctgtacaga attcttgcaa
      901 aggattatga aaactttgta aacatacaga agatggtgga cagacggaag gctgcagtga
      961 attattttgc agtaaaatcc agcgcattct ggaaactgat gagtcaggaa cgtctctgtt
     1021 ccatcatcgt gtttcttgtg agagatgtac atgaatacaa acttgctgct cagtttctga
     1081 tgatcctttc acctgaagtc atttctcagt ttacaaagtt tacaaatctg agtgaagatg
     1141 acgaacgaaa tcttttcctt gcactcgaag acaatatata taatatccct ctggtttcac
     1201 ctaaaatata cgggcatatg cttgagcttt tcaaggatga attcgagatc ttttttgtgc
     1261 tggaaacaat gggaccctta gtcaagagaa gggctgaaat cgaagatatc agtatgtcct
     1321 ttctgcgtta ccataaaaga accggacaga aactttccat tcagtggatt tattctgaac
     1381 tgttcggtct ggagtatgag cttgttgtgg agattctgaa ccagctgatg gaaaatgaat
     1441 tcattactct ttctg
//
LOCUS       DAOQGJ010001265         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68218_length_1454_cov_3.228735, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001265 DAOQGJ010000000
VERSION     DAOQGJ010001265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68218_length_1454_cov_3.228735"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK683_17005"
     CDS             <1..316
                     /locus_tag="PK683_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNH10200.1"
                     /translation="PANFLDVGGGANVTTVTNGFKIILGDPNVKAIFVNIFGGIVRCD
                     RVAQGIVEAAKAVNIRVPLMVRLKGTNAEEGKKILSDSGLTIIVADELSDAAEKIPAL
                     IK"
     gene            326..1213
                     /gene="sucD"
                     /locus_tag="PK683_17010"
     CDS             326..1213
                     /gene="sucD"
                     /locus_tag="PK683_17010"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNH10201.1"
                     /translation="MAVLIDKNTKIVVQGITGKEGSFHTSQMIEYNTPFGSKVVAGVT
                     PGKGGTKSEHGVPVFNTIKDAISAEGANVSAIFVPPAFAADAILEGIFAEIPLIICIT
                     EGIPVHDMTRVYNVLKHSKTRLIGPNCPGVISPGKCKIGIMPGFIHKEGRIGVVSKSG
                     TLTYEAVGQLTNAGLGQSTCIGIGGDPIIGSSHIDALKLLNEDPDTDGIIMIGEIGGT
                     AEEEAAAFVKANIKKPVVGFIAGQTAPPGKRMGHAGAIISGGMGTAQSKMAAMRDAGI
                     HVCQSPADMGATMKKVLGK"
     gene            1215..>1454
                     /locus_tag="PK683_17015"
     CDS             1215..>1454
                     /locus_tag="PK683_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10202.1"
                     /translation="MKKLRTVFLIFLFAGALNVLVGQAKKKTQEETAEVQNRLKLSMK
                     ELVQKAVENSPEIRNTSFELIKTDSGFLKSQSKYSW"
BASE COUNT          464 a          301 c          376 g          313 t
ORIGIN      
        1 accagccaac tttctggatg taggcggcgg ggccaacgtc acaacagtga ccaacggctt
       61 caagatcatc ctcggcgatc cgaatgtaaa ggccatcttc gtaaatattt tcggaggaat
      121 tgtccggtgc gacagggttg cacagggtat cgtggaagcg gcaaaggctg tgaacatcag
      181 agtgcctttg atggtgcgtc tgaaaggaac aaatgctgag gaaggaaaga aaatcctctc
      241 tgacagcggc cttactatca ttgttgcaga cgagctttcg gatgcggcag aaaaaattcc
      301 agcactgatc aaataagagg gaaaaatggc agttctcata gacaaaaata caaaaatcgt
      361 agttcagggg atcacaggaa aggaaggttc atttcatact tcccagatga tcgaatacaa
      421 tactcctttc gggtccaagg tagttgcagg agtgactccg ggaaaaggcg gaaccaaaag
      481 tgaacatggg gttcctgttt tcaatacaat caaggatgcg atcagtgcgg aaggtgcgaa
      541 tgtgagcgca atctttgttc ctcctgcatt tgcagcagat gcaatcctcg aaggaatctt
      601 tgcggaaatt cctctgatca tctgtatcac agaagggata cctgttcatg atatgaccag
      661 agtctataat gttctgaaac attcaaagac aagactcatc ggaccgaact gtccaggagt
      721 catatcaccc ggcaaatgca agataggaat tatgcccgga ttcattcaca aagaaggaag
      781 aatcggtgta gtatccaagt cgggtactct gacatatgaa gcagtcggac agctcacaaa
      841 tgcaggactc ggacagtcca cctgcatagg gatcggcgga gatcctatca tcggttccag
      901 tcatattgat gctctgaaac tcctgaatga agatcctgat acggacggga tcattatgat
      961 cggagaaatc ggggggacag ctgaggaaga agccgcagca ttcgtaaagg caaacataaa
     1021 aaaaccggta gtcggattca ttgcaggaca gactgcacct cccggaaaaa gaatggggca
     1081 tgcaggagca atcatcagcg gcggtatggg aacagctcag tcgaagatgg ctgcaatgag
     1141 agatgcagga attcatgtat gtcagtcgcc tgcagatatg ggtgcaacaa tgaaaaaggt
     1201 gctcggaaaa taaaatgaaa aaactcagga ctgtattctt aatatttctt ttcgcaggag
     1261 ctctgaacgt actcgtgggt caggcaaaga agaaaacaca ggaagaaact gcagaggttc
     1321 agaacagact gaaactctcc atgaaagaac ttgtgcagaa ggcagtggaa aacagtcctg
     1381 agatcagaaa cacatccttt gaactgatca agacagacag cggcttttta aagtcgcagt
     1441 caaaatactc ctgg
//
LOCUS       DAOQGJ010001266         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68244_length_1454_cov_2.406719, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001266 DAOQGJ010000000
VERSION     DAOQGJ010001266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68244_length_1454_cov_2.406719"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..>1454
                     /locus_tag="PK683_17020"
     CDS             208..>1454
                     /locus_tag="PK683_17020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH10203.1"
                     /translation="MPEHTTLYQYLSATSARFPDKISFLKRNSTGNFQGKSFASLKKS
                     VDELCAGIQAEGIRPAEKIALFCDSSPNWFFTDLSIITSGCVSVPRGTDVTDEDIEYI
                     INHAECRTAIVQNEKTRDRLVSLRSKIPILEKIYILEKDAFHLYAGNDSVQSLMEKGR
                     EFLKTKQDEAQKRIESVNPEELATLIYTSGTTGAPKGVMLNQRGWITAIQSTIGCVGI
                     TSEDRALSLLPPWHAFERAVEYAVIFLGAEFLVTDIKTLKEDLRDFKPTFFPSVPRIW
                     ESLYNGIKAKVGLESRTKQKLFETALKAGKFWSKQKAVLLGYNFSMFKPGIFSILFSR
                     TGALLSLIPASPGKLLSILVFKKIHEAFGGNLKISISAGSALPGAVDEFLSAIGLTVL
                     EGYGMTETSAVISVRKKDSPTPST"
BASE COUNT          447 a          329 c          306 g          372 t
ORIGIN      
        1 accgcccacc tcgacctgtg gtgagctgag tcgaatccat acgaaaaacc ctgaaaatat
       61 tgggtttttc atacctcgac aagctcggca catcgctcgg tgaccggcgg taaaaactgt
      121 atcaatgtat gcttttacac aacctcactg acagctcacg atgctaaaaa tccctttaca
      181 aaattcattt ccttctaaaa tgaaggaatg cctgaacata caacccttta tcaatatctt
      241 tctgcaacat ccgccagatt tccggataaa atttctttcc tgaaaaggaa cagtacagga
      301 aactttcaag gaaaaagttt tgcatctctg aaaaaatctg tagatgaact ttgtgccgga
      361 attcaggcag aaggaatcag acctgcagaa aagattgccc tgttctgtga ttcctctccc
      421 aactggtttt tcactgatct ttccatcatc acatccggat gtgtgtctgt tccgagggga
      481 actgatgtca ccgatgaaga tatagaatat atcatcaatc atgcagaatg caggacagca
      541 atcgtacaga acgaaaaaac aagggacaga ctcgtttctc tcagatccaa aattccgatt
      601 ctcgaaaaaa tttatatcct ggaaaaggat gcatttcatc tgtatgcagg aaatgattca
      661 gttcagtctc taatggaaaa aggcagagaa tttctgaaga caaaacagga cgaggcacag
      721 aaacgaattg aatctgtgaa cccggaggaa ctggcaactc tgatttatac aagcggaaca
      781 acaggtgctc cgaaaggagt tatgctgaat cagagaggct ggatcactgc aattcagagc
      841 acgatcggat gcgtaggaat tacttccgaa gacagggcac tgagcctgct ccctccttgg
      901 catgcatttg aacgggctgt tgaatatgct gtgatattcc tgggtgctga atttcttgtc
      961 acagacatca agactctgaa ggaagattta agagacttca aaccgacttt ctttccttca
     1021 gttccccgaa tctgggaatc cctttacaac ggaatcaagg caaaggtggg tctcgaatcc
     1081 cggacaaaac agaaactatt cgaaactgca ctcaaggcag gaaaattctg gtcaaagcag
     1141 aaagcagttc tcctgggata taatttcagt atgtttaaac cgggaatatt cagcattctt
     1201 ttcagcagaa caggagctct tctcagcctc attcctgcat cgccaggcaa actgctttcc
     1261 attcttgtat tcaaaaagat tcacgaagca ttcggaggca atcttaagat atccatttct
     1321 gcagggagtg cacttccggg tgcagtagat gaatttcttt ctgcaatcgg tctgacggtt
     1381 ctggaaggat atggaatgac ggaaacttct gcagtgatct ccgttcgaaa aaaagacagc
     1441 cccactccat ccac
//
LOCUS       DAOQGJ010001267         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68326_length_1453_cov_2.452074, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001267 DAOQGJ010000000
VERSION     DAOQGJ010001267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68326_length_1453_cov_2.452074"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..>1453
                     /locus_tag="PK683_17025"
     CDS             267..>1453
                     /locus_tag="PK683_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10204.1"
                     /translation="MSCAWFYLPALPKIVRYYSKWTDYADGQYHLISISDRLFFYAKN
                     FLFNHLTVPGTVFLLVFFLLSAVWTAFRKNREDIIQDYKESIKNSRIILIFAGYFSTV
                     VFLTLRSSFSTAADNPLLPVFFSLIAAVSGGVTKRESFSPALYSCAVFVLLLFFLNSV
                     SKIPKEIIPDKAEFISFASAVREFRQRYLIQNGRMIQSFSHPVYNTDTYRWSELLNVK
                     SNTEKTVKSAVSSEDWSDVLTLKDMQKTYSILRKYDMLLMSDSNPKLFGGEYFTQVNL
                     NWEELSAQIYKSDSFYCPEKFYIKSQSVHLSMCINRNFVGIEIPEVYSDGWIVWNTPV
                     KLFSYEGKILTAGGIPVRHITEFLMENENREEKIVFRLKNVDSQGNYNFEGFIKGDQN
                     IRS"
BASE COUNT          424 a          254 c          287 g          488 t
ORIGIN      
        1 atcagaattt tttccagaca aggaatgatt ttatattggg cttttttatt attctattca
       61 attatttagt tgtactgtct ttgaagcaga aggatgcatc tgtaatgttt tatgcaggta
      121 tttctgccgg aataggaact ttgttcaagc tcagtgctcc gggatatttt atttggatct
      181 tcatcttttt tcttattata cctgatgtcc ataattcatt ttttgttcgt ttaaagtgtc
      241 tgcttttggc ggttgccggc gcagttttgt cctgtgcttg gttttatctt cctgctctcc
      301 caaaaatagt cagatactat tccaagtgga cggactatgc agatggacag tatcatctta
      361 ttagcatatc agacaggctg tttttttatg ccaaaaactt tctgtttaac catttaacgg
      421 ttccaggaac tgttttcctt ctggtatttt ttcttctgtc tgcagtatgg acagccttca
      481 gaaaaaacag ggaagatatt attcaagact acaaagagag tataaaaaat tccagaatta
      541 tactgatctt tgcaggttat ttctctacag tagtcttttt gactttgaga agttcctttt
      601 caacagcggc agacaatcct ctgctccctg tttttttctc cctgatcgca gccgtttcag
      661 gaggggttac aaaaagagaa tcattttctc ctgcactgta ttcatgtgct gtttttgttc
      721 tgctgttgtt tttcctgaat tcagtcagca aaatacccaa agaaatcatt ccggacaaag
      781 cagagtttat aagttttgct tctgcggtca gggagttcag acagagatat cttattcaga
      841 atggcagaat gatacaatct ttttctcatc cagtatataa tactgatact tatagatggt
      901 ctgagcttct gaatgtaaaa agcaatacag aaaaaactgt gaaatcggca gtcagttccg
      961 aagactggtc agatgttctt acccttaaag atatgcagaa aacttattca attctcagaa
     1021 aatatgatat gcttcttatg tcggacagca atccgaaatt gttcggagga gagtatttta
     1081 cacaagtcaa tctaaactgg gaggaactgt ccgcacagat atacaagtca gattctttct
     1141 actgtcctga aaaattctat ataaaaagcc aaagtgttca tctctcaatg tgcattaaca
     1201 ggaattttgt aggaattgaa attccagaag tctattcaga cggatggatt gtctggaaca
     1261 ctcctgtaaa acttttctct tacgaaggaa aaatactgac agcgggcgga ataccagtga
     1321 ggcacattac cgagtttttg atggaaaatg aaaataggga agagaaaatt gtattcagac
     1381 tgaagaatgt ggacagtcaa ggcaattaca attttgaagg gttcattaag ggggaccaga
     1441 acattaggag ttt
//
LOCUS       DAOQGJ010001268         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68331_length_1453_cov_2.385551, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001268 DAOQGJ010000000
VERSION     DAOQGJ010001268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68331_length_1453_cov_2.385551"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PK683_17030"
     CDS             complement(<1..718)
                     /locus_tag="PK683_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10205.1"
                     /translation="MVFFFIPFISIKPTDKTENTLESYKEKYYSYKADADPKKNNANA
                     YKNIFNLSREIDTRLDSLEKNEPSDDERALIIELGALSNKVRLDLKRFELASDSEAQK
                     KDVQLRCEEENNSAAYMGERYRPFNSVFKFLGSKLTSCNIGILPVSITAIYGRGKDEY
                     VDKASFNPWGMVSIEEGRSKKNYLFYSNTLYIRFNPIYGLKFFVGPTVGIGVGTDSKF
                     SSQNPVLMYGPTVGITLDDTK"
BASE COUNT          401 a          271 c          265 g          516 t
ORIGIN      
        1 atttggtatc atccaaagtg attcccacgg tcggtccata cattagtaca gggttttgtg
       61 aactgaactt tgagtctgtt ccaactccaa ttcctacagt aggtcctaca aaaaatttca
      121 atccatagat tgggttaaat cttatataca gggtgttgga atagaacaga taattttttt
      181 tcgatcgccc ttcttcaatg ctgaccattc cccaaggatt aaaagaggct ttatctacat
      241 actcatcttt gccccttccg taaatagctg ttatacttac tgggagaata cctatattgc
      301 acgaggtcaa cttagagcct agaaacttga atacagaatt aaaaggtctg tacctttcgc
      361 ccatatatgc ggcagaatta ttttcctctt cacatcttaa ttgaacatct tttttctgtg
      421 cttctgaatc ggatgcaagt tcaaagcgtt ttaaatcgag tctgactttg ttagataatg
      481 caccaagttc tataatgaga gctctttcat catccgaagg ttcatttttt tctaatgagt
      541 ctaaacgggt gtctatctct ctacttaaat taaatatgtt tttgtaggcg tttgcattat
      601 tttttttagg atcggcatcc gctttgtaag aataatattt ttctttgtaa ctctctaagg
      661 tgttttctgt cttgtctgtc ggttttatag aaataaatgg aataaaaaag aaaaccaata
      721 atactaggtg attatacccc tttgtttcgc tgtttcgctg tttcgctgtt tcgctgtttc
      781 gctgtttcgc tgttaaacat actgatcctc aatttaaccg gaatattgca cttttgaatt
      841 tatacaataa aaaattgaac ttttcggaat taaattcttt cagtttgtca gataagccag
      901 ggttgtcttt ttgtaaatac ccaatgttgg gtattttttc gggattctac ggaaaaaatg
      961 cagagtcaag ataaattatt gatttttacg aacttattga gtggtttatt tttctcagcc
     1021 ggaaagggtt ttgtaaaaat tcagtttatt acaaaatatt ataatatgct gatcggattg
     1081 gaatcattgc tccgcgtctc cgcgtgatac ttatttctta cccgtttatt tgacccattc
     1141 gaaatgaatg cactcagcag cagagacttc tgcggggcta tactgatgtc ggattgtagg
     1201 aactcctacc ggtcatactg caggaactcc tacggggttg tgcggatgtt agatcgtaag
     1261 aactcctgca aggctaacta ttttctgctt cacagtaaga aatttttttt gcactcagat
     1321 tcaactattg acagcagtaa taattcagac agcttctttc acatacttaa tcgtcacaag
     1381 gtttgaactg aattcctgat ccagttcaag ctcaatcatt tggaaagtgg atggtgagca
     1441 aagcctggcc ttt
//
LOCUS       DAOQGJ010001269         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68474_length_1451_cov_3.830946, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001269 DAOQGJ010000000
VERSION     DAOQGJ010001269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68474_length_1451_cov_3.830946"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..787
                     /locus_tag="PK683_17035"
     CDS             83..787
                     /locus_tag="PK683_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10206.1"
                     /translation="MSDRKSSRRKKIERSKDDGLEQIRAHKEQVASEAKHTIYPASSV
                     SVHWEVLHTKRKIDPELKELVADYYYGIQKNPGKQISELEKTVETYNDIQLFQDYLLT
                     AYVIEEEKDKAITLAQNLSRKYPDFIIGKIAAVELAILKGDLNSIPSILGDKYEPTAI
                     FPERAVFHIYEILHFQFAVGRYFALKGDVKKAEEYLNLIKEADEDHIFIKKLQKVIDK
                     NSGLKFYQRVIKKIKG"
BASE COUNT          459 a          277 c          269 g          446 t
ORIGIN      
        1 ggtacagtaa ttcgttcgct gtcataattt tttcttgtaa taaaataccc gttttgccag
       61 ccttttgtaa agagatattt ttatgtcaga cagaaagagc agcagacgca agaaaattga
      121 gagatccaag gacgatgggc tggagcagat cagagcccat aaagaacagg ttgccagtga
      181 agcaaaacat actatttacc ctgcttccag tgtaagcgtt cactgggaag tactgcatac
      241 caaaagaaaa atagatccgg agttaaagga actggttgct gattattact acggaatcca
      301 gaaaaacccg ggaaagcaaa tttcggaact ggaaaagact gtagaaacct acaatgacat
      361 tcagcttttt caggattatc tgcttactgc ttatgtaatc gaagaagaga aggacaaggc
      421 aattacactc gcacagaacc tttccagaaa gtatcctgat tttattatcg ggaaaattgc
      481 cgctgtagaa ctggcaattc tgaaaggaga tctgaattca attccttcca tcctgggtga
      541 taaatatgaa cccactgcca tttttccaga aagagccgtc tttcatatct atgaaatcct
      601 gcatttccag tttgccgtcg gcagatattt tgctctgaaa ggtgacgtta aaaaagcaga
      661 agagtatttg aacctgatta aggaagcaga cgaagatcat attttcatca agaaactgca
      721 gaaagtaatt gataaaaatt ccggtctcaa gttctaccag agagtcatca aaaaaatcaa
      781 aggctagctg ttttcagttc agaatttcgg tctgcggcat aaaggctgtc gcggactgat
      841 tctgtgttta tctgcagaaa tattttttaa aaaatctgcc cccggaaatg ttctgttcat
      901 tggaaaaatt ttatcagata gttgttccga aaaaaatacg gcttcattga atgtaccgcc
      961 attttcggat cttttttatt tgatttcctt ctttctttcg gagaacgcac ccctgtaaca
     1021 acgttatatt ttaagtcctt gacccctctg aacttaccct gcaatgcagt ctttccttgc
     1081 ggtaaatgta attccgatag attcgatttc cagtttcttt aatagtaact tcctcattca
     1141 tggaaagtct ttctcctctt ttcagaatga ttgatttacc gtctatatgc acctgcgctt
     1201 tcctattgac aaattgaaca aaggctgttt ttgaattgtc tgcataaaga ctaaaagaca
     1261 acattagtga aaactgctcc ttcatttaaa atacctgtac caaagagcgg tttcatttta
     1321 taaaacagga aatcagtgtt ttttgttctg aaatttctca tctcataata ttcactcacc
     1381 ttacgcaaat acgatgttat gtctagatgc aataattttt aggaaacatt agggaaatct
     1441 aatgaattag t
//
LOCUS       DAOQGJ010001270         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68675_length_1449_cov_2.322095, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001270 DAOQGJ010000000
VERSION     DAOQGJ010001270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68675_length_1449_cov_2.322095"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK683_17040"
     CDS             complement(<1..270)
                     /locus_tag="PK683_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10207.1"
                     /translation="MAKFITPKKNRIYLETFTSLYPKFAGGTDSPEFKLIFHYLIQNI
                     DKMIWAIEDLNKPPLEGIGKRLVELNPGLFENPDIRRMTGALIAAI"
     gene            complement(285..>1449)
                     /locus_tag="PK683_17045"
     CDS             complement(285..>1449)
                     /locus_tag="PK683_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH10208.1"
                     /translation="IAVLSPLIAWVPIAALAGILLVVAFRMFDWKAFRLLKAKETRFD
                     FAVIAAVVIAAESFGLIAASGTGIGLSILLFMRDQIRISILRRRATLKDISSKTYRLE
                     PELALLEKYGSEAAVYELQGNLFFGTTDHLFTQLEDDLKHCKFILFDMKRVQSLDYTA
                     AHLFSLMYDRLKEHEGRLLFSDMPASLPTGQDVLKYLTDVGLVNGEKGGIRIFDTKDD
                     AIEWMENRLLKWYGAPAKTEEKALDLTEIELLYRTDEETIDALRKCIHEHSFKEGEKI
                     FGIGDDGDEIFMIRRGVVQVFLPLKTGKHLHLATFGQGDYFGEMAFLDKHSRSADAVA
                     KTDCDLYVLSRKEFNEQVQRDAVLGVRIFARIANVISLRLRQADIELTALEDG"
BASE COUNT          396 a          349 c          281 g          423 t
ORIGIN      
        1 gattgctgca ataagagcac ctgtcattct ccgaatgtca ggattttcaa acagaccagg
       61 attcaactct acaaggcgtt ttcctattcc ttccagaggc ggtttgttca aatcttctat
      121 agcccagatc attttatcaa tgttctgaat caaataatga aatattaatt taaattcagg
      181 tgagtctgta cctcctgcaa atttcggata cagacttgta aaggtttcca gataaattcg
      241 atttttctta ggagtgataa atttcgccat atgttttttc catctcatcc gtcttcaagg
      301 gcagtgagtt ctatgtctgc ctgtctgaga cggagagaga ttacattggc aattcttgca
      361 aatatcctta cgcccagaac tgcatctctc tgcacctgtt cattaaattc tttccgggag
      421 agaacataga gatcacagtc tgtcttagca actgcatcag cagatctgct gtgtttgtca
      481 agaaatgcca tctctccgaa atagtctccc tgtccgaatg tagccagatg aaggtgtttg
      541 cctgtcttca acggaaggaa aacctgcacg actccgcgac ggatcataaa aatctcatca
      601 ccgtcatcgc caattccaaa aattttttca ccttccttga aactgtgctc atgaatgcat
      661 ttcctcagtg catcaattgt ttcttcatcc gttctgtaca gaagttcgat ttcagtgaga
      721 tccagtgcct tctcttctgt ctttgcaggt gcaccgtacc atttcagcag acggttttcc
      781 atccattcaa ttgcgtcatc ctttgtatcg aatattctta tcccgccctt ctccccattg
      841 acgagaccga cgtcagtcag atatttcaga acatcctgac ctgtaggaag gctggctggc
      901 atatcgctga aaagcagacg gccttcatgt tccttcagcc tgtcatacat cagtgaaaat
      961 aaatgcgccg cagtatagtc caacgactga actctcttca tatcaaatag aataaacttg
     1021 cagtgtttca aatcatcttc cagctgagtg aagagatgat ctgttgtgcc gaagaacaga
     1081 tttccctgaa gctcatatac agctgcttct gatccgtatt tttccagaag agcaagttcc
     1141 ggctccagtc tgtacgtttt ggaagaaata tctttgaggg ttgcccttct tctaagtatg
     1201 gaaattcgaa tctgatcccg cataaacaga agtatggaaa gaccgattcc ggtccccgat
     1261 gctgcaatca gtccaaatga ttcagctgca atgacaactg ctgcaatgac agcgaaatca
     1321 aacctggttt ccttggcttt gagaagtctg aacgccttcc agtcaaacat tctgaacgcc
     1381 actacaagaa gtattcctgc cagagcagca atcggaaccc aggcaatcag aggggaaagt
     1441 acagcaata
//
LOCUS       DAOQGJ010001271         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68677_length_1449_cov_2.277618, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001271 DAOQGJ010000000
VERSION     DAOQGJ010001271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68677_length_1449_cov_2.277618"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PK683_17050"
     CDS             <1..729
                     /locus_tag="PK683_17050"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH10209.1"
                     /translation="LMKNRSRSEKYRIDFLIEQKKKFVFREEIHQLGGDVCIAGDLNF
                     AGRRHLMFFNGDAMGKSIQGAGGVLVAGSVLNSIMARNSSEARIKSVRPEEWLKNAFS
                     ELQIIFEAFEGCMLLSCILGLADEDTGEVIFFNAEHPYAVLYRDGKAEFAENSVGLYK
                     LGIPFQREFSLKHVRIAPKDVLLIGSDGKDDLLVGNSMNEEAELFLRIAEAAGSDPET
                     MLGLIRESGEILDDISIVRIERLT"
     gene            827..>1449
                     /locus_tag="PK683_17055"
     CDS             827..>1449
                     /locus_tag="PK683_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002759053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNH10210.1"
                     /translation="MVEFTKRAKKVINEMAQEEARRLGHDFVGPEHIFLGLLKEEDSV
                     AVKILHNLNVNTSDLRKEIERKSREENLILDLPASQERYQKIVEASQDEAKKMKHNYV
                     GTEHILLALLRDNNNLVAGILSSTGINYNVIKGEIHRLLGVSNAGVGVSAGGPQQSSS
                     QQTQGRQEKSKTPILDEFARDLTQLAKDKKLDPVIGREKEIERVIQIL"
BASE COUNT          461 a          265 c          365 g          358 t
ORIGIN      
        1 ctgatgaaaa acaggagcag atctgaaaaa tacagaatcg attttctgat cgagcagaaa
       61 aagaagtttg tgttcagaga ggaaattcat cagctggggg gagacgtatg cattgcaggc
      121 gatcttaatt ttgccggaag aaggcatctc atgtttttca acggcgatgc aatgggaaaa
      181 tcaattcagg gagcaggagg cgtgctggtc gccggttcag ttctgaactc aatcatggca
      241 cgcaattcct ctgaagcaag gattaagtct gtccgtccgg aggaatggct gaaaaatgca
      301 ttctctgaac ttcagatcat ctttgaagct tttgagggct gcatgcttct ctcctgcatt
      361 ctgggacttg ctgatgagga tacaggggag gtcatttttt ttaatgcaga acacccttat
      421 gctgttctgt accgggacgg aaaggcagaa tttgcagaga attctgtcgg actttacaaa
      481 ctcggcattc cgtttcagag agaattcagt ttgaagcatg tcagaattgc acctaaagat
      541 gtgctcctga tcggatcgga cggaaaagac gacctccttg tcggaaattc aatgaacgag
      601 gaagctgaac tgtttttaag aattgcagaa gcggcaggat ctgatccgga aacaatgctc
      661 ggtctgattc gggagagcgg agagatactg gatgatattt caattgtcag aattgagcgt
      721 ctcacttgaa aaaaataaaa atacttggaa ttttagaatg gaaatttata tggtagactg
      781 tacttgtcga aattagaagc gtctaaatta aaaaaggcag gatagtatgg tcgagtttac
      841 aaaaagagcc aagaaagtaa ttaatgaaat ggctcaagag gaagccaggc gtctgggaca
      901 tgattttgta ggccctgagc atatttttct aggtctcctg aaggaagagg attcagtagc
      961 tgtaaagatt ctgcataatt tgaatgtcaa tacttctgac ttaagaaagg aaattgagag
     1021 aaaatccaga gaagaaaatc tgattttaga ccttcccgcc agtcaggaaa gataccagaa
     1081 aattgtggaa gcatctcagg atgaagccaa aaaaatgaag cacaactacg tagggactga
     1141 gcatatcctt ctcgcccttc tcagagacaa caataatctg gtggcaggga ttctttcgag
     1201 tacaggcatc aattataatg tcatcaaagg agaaatccac agacttctgg gagtctccaa
     1261 tgcaggggtc ggtgtcagtg ccggcggacc tcagcagtct tcttcacagc agacacaggg
     1321 cagacaggaa aaaagcaaga ctcctatcct ggatgagttt gcaagagatc tgactcagct
     1381 tgccaaggac aaaaaactgg atccagtcat cgggagagaa aaagagatcg aaagagtcat
     1441 tcagattct
//
LOCUS       DAOQGJ010001272         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68713_length_1448_cov_3.785355, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001272 DAOQGJ010000000
VERSION     DAOQGJ010001272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68713_length_1448_cov_3.785355"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1252
                     /locus_tag="PK683_17060"
     CDS             122..1252
                     /locus_tag="PK683_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH10211.1"
                     /translation="MKQNELQNQLNWKIDNGNLQIKLEKVLDQENVSAFWPESEIIQK
                     KETFSTVRIEAESLEVCEGIGISFIFHWNKYCEIHSRAFEISGLRKEFQTLLSQFSAS
                     DFDLKTESAPTHHFVEDVGVRFDEQVQKAANVIEFIGETVSVMGKAFVNPERIRWKEV
                     LKIMEQTGVDALFIIALIGFLLGLIMSFQSAIPMQRFGAEIFVANLVALSLFRELGPL
                     MTSIILAGRSGSSFAAELGTMKVSEELDALNTMGLNPIQFLVLPRLIAASIITPLLSI
                     FFNFFGLVGSAIVILSFGYPMVTFTNQIARSVSIVDLAGGMFKSYIFGILVAGIGCLF
                     GIRTKEGASAVGESTTGSVVAGIITVCVADGIFSVFFFYLGI"
     gene            1252..>1448
                     /locus_tag="PK683_17065"
     CDS             1252..>1448
                     /locus_tag="PK683_17065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH10212.1"
                     /translation="MPSIIEVKNLTVSFGPQKILDDISFQVQQGEVFVILGGSGCGKS
                     TLMKHMIGLLKPAAGSILIEG"
BASE COUNT          393 a          278 c          344 g          433 t
ORIGIN      
        1 ccttgaattt acaaggattt tttcagttga aaaaaatctt tttagtcgaa atatgtaatt
       61 cccagtctat tctgtaggaa agaaaatgaa taccttaata tgccggaact ttagtcagta
      121 gatgaaacag aacgaactgc agaatcagct gaattggaaa atcgataacg ggaacctcca
      181 gataaagctg gaaaaagtct tagatcagga aaacgtatcc gcattctggc cggaatcgga
      241 aatcattcag aaaaaagaga ctttcagcac agtcagaata gaagcagagt ctttggaggt
      301 atgtgaagga attgggattt cttttatttt tcattggaac aaatactgcg aaattcacag
      361 cagagccttt gagatttccg gccttagaaa ggaatttcag actcttctct ctcagttttc
      421 cgcttccgac tttgatttga aaactgaaag tgcccccact catcattttg tggaagatgt
      481 gggtgtccgc tttgacgaac aggtgcagaa agcagcgaac gtcattgaat ttataggaga
      541 aactgtttct gtaatgggaa aggcatttgt caatccggag agaatacgct ggaaggaagt
      601 cctcaaaatt atggagcaga cgggggtgga tgcacttttc attatagcac tcattggttt
      661 tctgcttgga ctgatcatgt cctttcagtc tgcaattcca atgcagagat tcggtgcaga
      721 aatttttgtg gcgaatctgg tggcactttc actgttcaga gaacttggac ctttgatgac
      781 atcaatcatt cttgccggca gatccggttc ttcctttgct gcagaactcg gtacaatgaa
      841 agtcagtgag gaactggacg cactgaatac aatgggactg aatccgatac agttccttgt
      901 tctgcccaga ctgattgctg catccatcat tacgcctctg ctatccatct tttttaattt
      961 ttttggactg gtggggtcag cgatcgtaat cctttctttc ggttatccta tggtgacatt
     1021 tacaaatcag attgcaagat cagtgtcgat tgtagatctg gccggcggaa tgtttaaatc
     1081 ctatattttc ggaatccttg tcgcgggcat cggatgtctg ttcggtatcc gtacaaagga
     1141 aggtgcaagt gcagtagggg agtccacaac cggttccgtc gtggcaggaa tcattactgt
     1201 atgtgttgca gacggaattt tttcggtgtt ctttttttat ctgggtatat aatgccttcc
     1261 atcatagaag tcaaaaatct gactgtctct ttcggtccgc agaagattct ggacgacatt
     1321 tcattccagg ttcagcaggg tgaagtgttc gtcattctgg gaggatccgg gtgcggcaaa
     1381 agtactctga tgaaacatat gataggactt ctgaaaccgg cggcaggttc cattctgatt
     1441 gaaggtat
//
LOCUS       DAOQGJ010001273         1446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_68906_length_1446_cov_2.492451, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001273 DAOQGJ010000000
VERSION     DAOQGJ010001273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68906_length_1446_cov_2.492451"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PK683_17070"
     CDS             <1..481
                     /locus_tag="PK683_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10213.1"
                     /translation="IVNSIRPAEKNSTEPVIVKETVIIKEEKKEETPLKGRVNLRKST
                     SVCGRINEEGYHFDDDIKFEFSANREVGTTQLEIWISECKNSSDTSCHTPFMKKEKDI
                     LPDWKGLSSSHPVELKKLMSDNGRVKIPTKYEWYHFAVIIKDRVVAEKTFQIRNSCE"
     gene            671..889
                     /locus_tag="PK683_17075"
     CDS             671..889
                     /locus_tag="PK683_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10214.1"
                     /translation="MKILKIIHGYPPVYNAGSEVYSQSMCSELSKSHQVEVVTREENP
                     YLPDFSVRKIGIHENLHLNFINKRFDKD"
     gene            968..>1446
                     /locus_tag="PK683_17080"
     CDS             968..>1446
                     /locus_tag="PK683_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10215.1"
                     /translation="MNHLSLGIIPVLKKNSIPIVFTLHDFWLMCPRGQFLQRNYGNKE
                     FFQLCSSQENRKCAENCYNMYFSDSEYCEEDAAYWEKWIEKRMDETRRVSSLTDLFIA
                     PSRYLRERFIREFDIPEHKIIYLDYGFPNEYLKPAAYMQTEKFTFGYIGTHIPSKGV"
BASE COUNT          475 a          252 c          313 g          406 t
ORIGIN      
        1 gatagtgaac agtatcagac ctgcagaaaa gaattcgact gagcctgtta tagtcaaaga
       61 gacggtaatt attaaggaag agaagaaaga ggaaactccc ctcaaaggaa gagtaaacct
      121 gaggaaatcc acttccgtat gcggcagaat aaatgaagaa ggctatcatt ttgatgatga
      181 tataaaattt gagttttcag cgaaccgaga agtcggaacg actcagctgg aaatctggat
      241 atctgaatgc aaaaattcgt ccgacacttc ctgtcatact ccttttatga aaaaggaaaa
      301 ggacattctg ccggactgga aaggtttaag ttcatcacat cctgttgaat taaaaaagct
      361 gatgagcgat aatggtagag taaaaatacc gactaaatac gaatggtatc attttgctgt
      421 catcatcaaa gacagagtag ttgcggagaa aacatttcag atcagaaatt cgtgcgagtg
      481 aggatggttg acccggggaa ggtaatgaag ctgcggctga aaggccgagc agctttgata
      541 tgaactgcag cagaatctat gaaaaaatct gagcacttct gtcgtgcagt attgcggaat
      601 tgaatttgtc tttgtttaaa tgcataattt ggcttgcacg gaaactgtgt tcccggaatt
      661 aatttctgat atgaaaatac tgaaaatcat tcatggatac cctcctgtat acaatgcagg
      721 ttctgaagtt tattctcagt ctatgtgcag tgaactttcc aagtcacatc aggttgaagt
      781 tgtgacaaga gaagagaatc cctatctgcc cgatttttca gttcggaaga tcggcatcca
      841 tgaaaatctt cacctgaact ttattaataa acggtttgat aaagattagt acaataataa
      901 aaatatagat tatagatttt ctaagattat cgaaagattt cagccggaca tcgctcatat
      961 tggacatttg aatcatctct cgctgggcat tattcctgtc ttgaaaaaaa attccattcc
     1021 gattgtattc actctccatg atttctggct gatgtgtcct cgaggccaat ttttgcagag
     1081 gaactacggg aataaggagt tctttcagct ctgcagcagt caggaaaaca gaaaatgtgc
     1141 agagaactgc tacaatatgt atttttccga ttcagaatat tgtgaagagg atgctgcata
     1201 ctgggagaaa tggattgaaa aaaggatgga tgaaacaaga agagtcagca gccttactga
     1261 tctgttcatt gctccttccc gttatctgag ggaaagattt atccgtgaat tcgatattcc
     1321 tgaacataag ataatatatc ttgattacgg atttccgaat gagtatctca agccagcagc
     1381 ttatatgcag actgagaaat ttacttttgg atatatcgga actcatatcc cctccaaagg
     1441 agtcaa
//
LOCUS       DAOQGJ010001274         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69236_length_1442_cov_3.320836, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001274 DAOQGJ010000000
VERSION     DAOQGJ010001274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69236_length_1442_cov_3.320836"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PK683_17085"
     CDS             complement(<1..240)
                     /locus_tag="PK683_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNH10216.1"
                     /translation="MDIRNPIGPYKKKEPLDEKAKRLLIEELEKFPEDLKRLLSFADS
                     VKYEEKTLRGVWTVKQVVHHLADSHMNAFIRMKLAL"
     gene            complement(261..491)
                     /locus_tag="PK683_17090"
     CDS             complement(261..491)
                     /locus_tag="PK683_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10217.1"
                     /translation="MIESKTEKTENDFKKTLKEKIDAIREQKAENSRSRKKKIPSESF
                     LKRQGNQILLESKGKFLQEGLYEPGDNQGYCG"
     gene            669..1205
                     /locus_tag="PK683_17095"
     CDS             669..1205
                     /locus_tag="PK683_17095"
                     /inference="COORDINATES: protein motif:HMM:NF037679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH10218.1"
                     /translation="MKKESAKGKLLESAKKILKEEGYEKFTLDSLAKKTGVSKGGVLY
                     HFPTKERLIQEIIENLIIRFENRLHSEENADSSGSWTLRYFQATLDDLDDSDLQSSGI
                     IAALASNPHLLLPLVEKYSAWKQKFKKEKMDQSLADILRFAADGIWFNEVFGLSVLSR
                     KEKKQILERAEKLAGQKK"
     gene            1202..>1442
                     /locus_tag="PK683_17100"
     CDS             1202..>1442
                     /locus_tag="PK683_17100"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH10219.1"
                     /translation="MKNFTGKKAAVIGATGGLGRAVSTALSIKGCSLVLIGRDTEKLI
                     QLQEEIKNLTGNTVYLQTVCRFDQYSEWEKAAAETE"
BASE COUNT          404 a          295 c          307 g          436 t
ORIGIN      
        1 gagtgcaagt ttcatcctga taaatgcgtt catatgactg tcggcaagat gatgtacaac
       61 ctgttttacg gtccagactc cccgcagagt tttttcttca tattttacac tgtctgcaaa
      121 ggaaaggagc cttttcagat cttccggaaa tttttccagt tcttctatca gcagtctttt
      181 ggccttttcg tccaaaggct cttttttctt atatggtcca atcgggtttc tgatatccat
      241 tttttccccc atttttacac tcagccgcag taaccctgat tgtctcccgg ttcatacagg
      301 ccttcctgta agaattttcc cttcgattcc agcagaatct gattcccctg ccgctttaaa
      361 aaagattcgc ttggaatctt tttcttcctt gagcgggagt tttctgcttt ctgttctctg
      421 attgcatcta ttttttcttt cagagttttc ttgaagtcgt tttccgtctt ttcggtcttt
      481 gattcaatca ctgatttttc tgcaggcctg agtgcagttt caatgcaaaa tctccctttt
      541 gcataaaggc ataggatcag tttatttctg agatctcttc tgaaaagctg tttttatctg
      601 gagagaaaag ccgagcgcag gaaaattttt tcttgacaac tgtaccgtct ggacggtata
      661 gttgttttat gaaaaaggaa tctgccaaag gaaaacttct ggagtctgcc aaaaaaattc
      721 tgaaagagga aggatatgaa aaattcactc tggacagtct agcaaaaaaa acaggtgtca
      781 gcaagggagg agtgctttac cattttccca ccaaggaacg gctgattcag gaaatcatag
      841 agaatcttat tatcaggttt gaaaatagac ttcattcaga ggaaaatgca gacagttcag
      901 gttcctggac tctcagatat tttcaggcca cactggacga cttggatgat tctgatctgc
      961 agagctccgg catcattgca gctcttgcat cgaaccctca tcttcttctt ccgctggtcg
     1021 aaaagtattc agcctggaag cagaaattca aaaaagaaaa aatggatcag agcctggctg
     1081 atatactcag gtttgccgcc gacggaatct ggttcaatga agtgtttgga ctgtctgttc
     1141 tttccagaaa ggaaaaaaag cagatactgg aaagagcgga aaaacttgcg ggacaaaaga
     1201 aatgaaaaat ttcactggaa aaaaagcagc agtcataggt gcgacaggag gacttggcag
     1261 ggctgtttcg actgcacttt ccataaaagg ctgttctctg gttctcattg gacgcgacac
     1321 tgaaaagctg attcagctgc aggaagaaat aaaaaatctc acaggaaata ctgtatattt
     1381 acagacagta tgcaggtttg atcaatattc tgaatgggaa aaggctgctg ctgagacaga
     1441 aa
//
LOCUS       DAOQGJ010001275         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69297_length_1442_cov_1.726027, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001275 DAOQGJ010000000
VERSION     DAOQGJ010001275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69297_length_1442_cov_1.726027"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK683_17105"
     CDS             <1..398
                     /locus_tag="PK683_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10220.1"
                     /translation="HRDMINKIVDWIDKLQKGQLEAEFIQGYLVTFQYYFENILLELR
                     EFEETQLCYIPPNSHEYFSFSGTEPKYLSVLQDFNPEQASEMKEKVIFYHKTTNIYVE
                     LIGLKITGDSNSVRLELENLYNYSAILSS"
     gene            800..1108
                     /locus_tag="PK683_17110"
     CDS             800..1108
                     /locus_tag="PK683_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10221.1"
                     /translation="MNLTFYEYSLFAGDPVFLPALERFFEDCGLDFSSAVQKHPELIQ
                     SLLFRKIESGKASLIFITSFEAFWKKESEGNSFEEICTLLGIAEEEDLFNTLKSLFLK
                     "
     gene            complement(1194..>1442)
                     /locus_tag="PK683_17115"
     CDS             complement(1194..>1442)
                     /locus_tag="PK683_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10222.1"
                     /translation="ALNFVIGAASSVKTESEKVLGQIEAKLRELSEKGAQDNSEVAMN
                     LRKYTEEAFREIDKLLTEARTRFEEVKEKAKGIIPPQQ"
BASE COUNT          418 a          259 c          292 g          473 t
ORIGIN      
        1 cacataggga tatgatcaat aagattgtgg attggattga caaactccag aagggtcagc
       61 ttgaagcgga atttatccag ggatatttag taacttttca atactatttt gaaaacatat
      121 tattggaact tagagaattt gaggagactc agctctgcta catccctcca aactcacatg
      181 agtatttcag tttttccgga acagagccta aatatctgag cgttcttcag gattttaatc
      241 ctgagcaggc ttcggaaatg aaggaaaaag tcatttttta tcataagacc acaaatatct
      301 atgtggaact tatcggtctg aaaatcaccg gggacagcaa ttcggtcaga ctggaacttg
      361 aaaatttata caattattcc gcaattctca gttcatgagt ttatgtcagc cggattcttt
      421 tttttcctgc ggatcctgct gcgggagttt caatctgaag cttaatagga aggatctttt
      481 cgaactgttc tgtgaaagga ctgaagaatt caggcagaca gcagattttt caaaaatctg
      541 gactctttcc gatttcagaa agaaaagaga atttattgaa gagaaaattg aacgaaatga
      601 ccctacgaca tataattgtc cctttttagg atttattgat tctggacatc ggaagatcgg
      661 ctgcctggtg catccatatg catcacataa tcccaggagt cagaatgtat cgttttacgg
      721 tgcttccata tgccaaggat atgactgcag aaacaaggaa ggcaaaaact cagaaatttg
      781 ggaaaaattt ttatgcagat tgaatctcac attttatgag tattctttgt ttgcaggaga
      841 tccagttttt cttccggcat tggaaagatt ttttgaagac tgcggtcttg atttttcctc
      901 tgccgttcaa aagcatcctg aactgatcca aagtcttctg ttcagaaaga ttgaatccgg
      961 aaaagcatca ctgattttta ttacgtcatt tgaagcgttt tggaaaaaag aatcggaagg
     1021 gaattccttt gaagagatct gtactttgct gggaatagct gaagaggaag acctttttaa
     1081 tacattgaag agtctatttt taaaataagg ggaggtttgc ccattcgaca gaaatcgaac
     1141 cagaatttct ctgtcccctt gttttttaaa tcacgttaag gttgactgtt tccttattgc
     1201 tgaggaggaa ttattccttt tgctttttct ttaacttctt cgaatctagt tcttgcttct
     1261 gtaagaagtt tgtcaatttc gcggaaagct tcttcagtat atttgcgaag attcatcgct
     1321 acttcactgt tgtcctgagc acctttttca gagagttctc tcagtttggc ttctatttgt
     1381 ccaagaacct tttcagattc agtttttaca ctggaagctg ctccaattac aaagttcagg
     1441 gc
//
LOCUS       DAOQGJ010001276         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69333_length_1441_cov_3.049784, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001276 DAOQGJ010000000
VERSION     DAOQGJ010001276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69333_length_1441_cov_3.049784"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1019)
                     /locus_tag="PK683_17120"
     CDS             complement(96..1019)
                     /locus_tag="PK683_17120"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNH10223.1"
                     /translation="MTEYSSDPFRAAEIVRGGGIAVFPTETVYGIGASSYNEDSCRKI
                     YKIKNRPSDNPLIVHFYSLEDVLDHIEVSPEILEKISRLVPGPVSFVAEKKTGSGIFT
                     SGLQSIAFRIPSLDLCRRFLKSAGPVSAPSANMSGKPSITRFRDAREVFHGKVDIILG
                     ESDTEIGLESSVIDIRRNPPLILRPGGLGIDSLKNIFSDIQIEKKYSEYVSSPGMKYR
                     HYSPDAEVILCEVLPEFPEKDSARIGFEMKNCSETDLVLSDNLQYMHELYSFLIDSDR
                     RKMKRIFCQSPLKDGFFDAILNRLKKASEKI"
     gene            complement(1016..>1441)
                     /locus_tag="PK683_17125"
     CDS             complement(1016..>1441)
                     /locus_tag="PK683_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNH10224.1"
                     /translation="ILAKHSGSKTWKNLTRFKYIHENKSYEDPSLWYRSGGCDRVAFY
                     MNRRAFEAKMTLLRKYELAGFSFWQLLSNNDPGINDYLSLLMTDKLPKVKTAKEEDAE
                     RKEKEALEEKLKAEKLAAEKASKPEKETAPEKSRERTAD"
BASE COUNT          372 a          330 c          292 g          447 t
ORIGIN      
        1 gctcattctt ctcactttat ggcttgcagt ggaaggaatt ctggtctatc ttcatttaag
       61 gaaaaaacac agactcggtg aataaaaaat cggcttcata ttttctcgga agcctttttc
      121 agtctgttca gaatcgcatc aaagaacccg tccttcaaag ggctctggca gaatattctt
      181 ttcatctttc ttctgtcaga gtcaatcaga aatgaataca gttcgtgcat gtactgaaga
      241 ttgtcgctca gaaccagatc ggtttcactg cagttcttca tttcaaatcc gatcctggct
      301 gaatcttttt caggaaattc cggaagaacc tcacagagaa tcacttcagc atccggggaa
      361 taatgtctgt atttcatccc tggactggag acatactctg aatatttctt ttcaatctgt
      421 atgtcagaaa aaatattttt taatgaatca atacccagcc cgccgggacg cagaatcaga
      481 gggggattcc ttctgatgtc gatgaccgat gattccagtc cgatttcagt atctgattca
      541 ccgagaataa tatccacttt tccgtgaaat acttccctgg catccctgaa tctggtgatg
      601 gagggttttc cggacatatt tgctgaagga gcactgacag gccccgccga tttcagaaat
      661 cttctgcaca gatcaagaga gggaatccga aatgcaatgc tctgcagtcc ggatgtaaaa
      721 attcctgaac ctgttttctt ttctgctacg aaagatacag gccctggaac aagtctgctg
      781 attttttcca gaatttcagg ggatacttca atatgatcaa gcacatcttc caaagaataa
      841 aaatgaacga tgagaggatt gtcggaagga cggttcttta ttttatatat ttttctgcat
      901 gaatcttcat tataggaaga tgcaccgatt ccatacacag tctccgtagg gaaaacggct
      961 attccccctc cccgcactat ttcggcggca cggaaggggt cagaggaata ttccgtcaat
     1021 cggctgtcct ttccctgctc ttctcaggag cagtttcctt ctcaggtttc gaagcttttt
     1081 ctgcagcgag tttttccgcc ttcagttttt cctcaagagc ttctttttcc tttctttcag
     1141 catcttcttc ctttgctgtc ttcaccttgg ggagcttgtc agtcatcaga agagaaagat
     1201 aatcattgat tccaggatcg ttattgctca gaagctgcca gaaagaaaaa ccggcaagtt
     1261 catattttcg gagcagtgtc atctttgctt caaaggctct tctgttcata tagaaggcaa
     1321 ccctgtcgca ccccccggat ctgtaccaga ggctcggatc ttcataggat ttgttttcat
     1381 gaatatactt gaaccttgtc agattcttcc atgtctttga acctgaatgt tttgcaagga
     1441 t
//
LOCUS       DAOQGJ010001277         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69461_length_1439_cov_4.458092, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001277 DAOQGJ010000000
VERSION     DAOQGJ010001277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69461_length_1439_cov_4.458092"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1294)
                     /locus_tag="PK683_17130"
     CDS             complement(47..1294)
                     /locus_tag="PK683_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002124856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNH10225.1"
                     /translation="MQIDSGTTAWMLVSTGLVLLMIPGLAMFYGGLVHTKNVLGTMMH
                     SFVPLAVIGVLWVAVGYSLAFGKSIAGGLIGWNWDYFFLKGIDESIVNGVPEYVVAMF
                     QGKFAIITPALISGAIAERVTLKGYIAFIAMWFLLVYCPLAHWVWASDGWLYTAGAAG
                     VIDLAGGLVIHVSAGTSALVAAVFLGKRYGFPHNTMNPNNLVMTLMGAGLLWVGWFGF
                     NAGSTVQSGLDTARALTMTQVSAASGALTWMIIETVKFRKVTSLGFVSGILAGLVVIT
                     PAAGVVLPGGALFLGAASSVICFFALELKNKLGYDDSLDCFGIHGVGSGFGVLALSFF
                     IRDSWMKAASVKAGTEWTGFSQFLVQLKGMGAVILLSALATYIICFIIQKTIGFRLSR
                     SEELAGLDHSEHSESGYGLINLN"
BASE COUNT          426 a          373 c          296 g          344 t
ORIGIN      
        1 attcctgcag aaagcttcaa aatcagatga aattcagatg cacggcttaa ttcagattga
       61 taagaccata accgctttca ctgtgctctg agtgatcaag tccggcgagt tcttccgatc
      121 tgctcagcct gaatccgatt gttttctgta taataaaaca tataatataa gtcgccaatg
      181 cagagagcag gatgacagca cccatgcctt tcagctgaac aagaaactgg ctgaatccag
      241 tccattcagt tcctgctttg acagatgcag ccttcatcca ggaatccctg atgaaaaaac
      301 tgagtgccag aactccgaat ccgcttccga ctccgtgaat tccgaaacag tccagactgt
      361 cgtcataacc aagtttgttt ttcagttcga gggcaaagaa acagatcaca gaagatgcgg
      421 ctccgagaaa cagtgctcct cccggaagca cgactcccgc cgcaggggtt atcacgacaa
      481 gcccagcaag aattccggat acaaaaccga gagaagtcac ctttctgaat ttgactgttt
      541 caatgatcat ccatgtgagc gcaccggagg ctgcagatac ctgagtcatc gtaagtgctc
      601 ttgctgtatc cagtccgctc tgaactgtgg aacctgcatt gaatccaaac cagcccaccc
      661 atagaagacc ggcacccatc agtgtcatta caaggttgtt cgggttcatt gtgttgtgag
      721 ggaaaccgta tcttttcccg agaaacacag cagccacaag agcactggtc ccggcagaaa
      781 catgaatcac aaggccgcct gcaaggtcaa tgactccggc agcacctgct gtataaagcc
      841 atccgtccga ggcccagacc caatgagcaa gaggacagta gacaaggaga aaccacattg
      901 caataaacgc gatataacct ttcaatgtaa ctctttctgc aattgcaccg ctgatcagag
      961 caggagtgat gattgcaaac tttccctgaa acattgcaac cacatactca ggcaccccgt
     1021 tcacaatgga ctcatctatt cctttgagaa aaaagtaatc ccagttccag ccgataagtc
     1081 ctcccgcaat acttttaccg aatgcaagtg agtatccgac tgccacccac agcacgccga
     1141 tgactgccag cggaacaaaa ctgtgcatca ttgtgcccag aacatttttg gtatgaacaa
     1201 gaccgccgta aaacattgcc agtccgggta tcattaaaag aactaggcct gtggacacca
     1261 gcatccacgc agtagtacct gaatcgattt gcatattttc cccttatcag ttttgataga
     1321 aaccaggcct tcctgatttc caaatgtcaa gaaactcatt ctcaataaaa aattctccgt
     1381 tttgtattta agatcattga gaagctgagt tttgaattaa agcacaggtt ttttttgtc
//
LOCUS       DAOQGJ010001278         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69521_length_1439_cov_2.269509, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001278 DAOQGJ010000000
VERSION     DAOQGJ010001278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69521_length_1439_cov_2.269509"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PK683_17135"
     CDS             complement(<1..520)
                     /locus_tag="PK683_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3939 domain-containing protein"
                     /protein_id="HNH10226.1"
                     /translation="MAKQEEQKRNFKKLSNVEHVRMRTGMWLGQNSLSTFEQHFFKKT
                     KETYEILHEELEDIPAKLKCLDEACMNAVDEYRKNLNDKTIQPKDKMSKLIVSLSADQ
                     KRVTVEDNGRGIPAENAEGVYLHLMYGENFDDKVKEDHVAGQNGVGISLVRMVSSFFR
                     VETSSKGKTYKKL"
     gene            complement(522..>1439)
                     /locus_tag="PK683_17140"
     CDS             complement(522..>1439)
                     /locus_tag="PK683_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001972951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNH10227.1"
                     /translation="TEDEPIHFVGKVPVVLLNHIQGIATGFRCFIPGHKLGDIIDSQI
                     AYLKTGKTKKIKPWYKDFGGEIRLSKNDNGSEILTTTFGFKWEGETLFLTDAPMSWNR
                     EKVITYLDDLLEKKDHWLKDYMDHSSQRFRIELLYKKGEKPSETQIRELFNKEQNEAL
                     TYNVITYEGKLKNETPEEIIRRFCDFRKNHLVRRFKRLAGLEQEKIDRNSELIRFIRE
                     KWNLKVTAIKSKSDFEDQLKKSKFTYFEWLASIPVYRMTLEEVKKCEEAIVEAKTRFD
                     EYTGLYKKDDRLTVFMVNELTELKNKWDK"
BASE COUNT          306 a          366 c          279 g          488 t
ORIGIN      
        1 acagcttctt ataggtcttc cccttgctgc tcgtctccac tctgaaaaaa ctggagacca
       61 ttcttacaag agaaataccc actccgttct gacctgccac atggtcttcc ttgaccttgt
      121 cgtcaaagtt ttctccgtac atcagatgca ggtacacccc ctccgcattc tctgcaggaa
      181 ttccccgtcc gttgtcttcc actgtcactc gtttctgatc ggccgaaaga gagacgatca
      241 gcttggacat cttgtccttc ggctgtatgg tcttgtcgtt cagattcttc cggtactcat
      301 ccactgcgtt catgcatgcc tcgtcgagac atttcagctt tgccggaatg tcctcaagct
      361 cctcatgcag tatttcataa gtttctttag tttttttgaa aaaatgctgt tcgaatgtgg
      421 acagcgaatt ctgaccgagc cacattcctg tcctcatgcg gacatgctcc acattggaga
      481 gtttcttgaa atttcgtttc tgttcttcct gtttggccat tttatttatc ccacttgttc
      541 ttcagttcag tcagttcatt caccatgaac actgtgagtc tgtcgtcttt cttgtacagt
      601 ccggtatact cgtcaaatct ggtctttgct tccacaatgg cttcctcgca ttttttcact
      661 tcttcgagag tcattctgta aaccggaatg gaagcaagcc attcaaagta agtaaactta
      721 cttttcttga gctgatcttc aaagtccgac ttcgacttga ttgctgtgac tttaaggttc
      781 catttctccc tgatgaagcg gatcagttca ctgttcctgt caattttttc ctgttccagt
      841 ccggccagtc tcttgaaccg tctgaccaga tgattttttc tgaaatcgca gaaccttctg
      901 atgatttcct caggagtctc attcttgagc tttccttcat acgtaattac gttgtatgta
      961 agtgcctcat tctgctcctt gttgaacagt tccctgatct gagtctctga tggcttttcc
     1021 ccttttttgt acagaagttc tattctgaat ctctggctgg aatgatccat atagtccttg
     1081 agccagtgat cctttttttc gagaagatcg tccagataag tgatgacctt ttccctgttc
     1141 caggacatag gtgcatccgt caggaacagg gtctctcctt cccatttaaa tccgaaggtt
     1201 gtggtgagga tttcagaccc gttgtcattt ttcgacagcc tgatctcacc gccgaagtcc
     1261 ttgtaccaag gctttatttt tttcgtcttt ccggttttca gatatgcaat ttgggaatca
     1321 atgatatcgc cgagcttatg gccgggaata aaacatctga aaccggtggc aattccctga
     1381 atatgattca gcaggacgac tggaaccttt cccacaaaat gaatcggttc atcttcagt
//
LOCUS       DAOQGJ010001279         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69623_length_1438_cov_2.347072, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001279 DAOQGJ010000000
VERSION     DAOQGJ010001279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69623_length_1438_cov_2.347072"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PK683_17145"
     CDS             complement(<1..1045)
                     /locus_tag="PK683_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10228.1"
                     /translation="MKKFLIFSVILAFSVLAEEIDSYKEGSDAFFQSQVDKLGKGSLK
                     ERIDAIGTLKKHRTLRALRPLILAVKGSVEIDADLVSLKENDKMKEKMLTPSEVKDGG
                     FGFYDKPEVIEKASRQILNPETVIPLDANIAEHNAPILKYLASQAIADLGHTRGMQPL
                     VDVFYQWQDRIKENDKPTYEKDENMRLVVGIAEVLRSIGTLIDNMDVKEESADEKKDK
                     KKTPILDSIKVGIDTLTFALGHPNYFIRAAAADGLRNTYRKEGMEKLLERLKTETDAS
                     KKLWDERTNRQKNIALAKRHDYVTVAILSAIIGIEPHASEKFLELTEMLKHDDPYIRL
                     RASIGLGDSGISNA"
BASE COUNT          383 a          305 c          284 g          466 t
ORIGIN      
        1 ctgcattgct gattcctgaa tcaccgagtc cgatggacgc tctcagacga atataaggat
       61 catcatgctt cagcatctca gtcagttcca ggaacttctc acttgcatga ggttcaatcc
      121 ctatgattgc acttaagata gcaacagtca catagtcatg tctttttgcc agtgcaatgt
      181 ttttctgacg gtttgttctt tcatcccaga gttttttgct tgcatctgtc tcagtcttca
      241 gtctttccag aagtttttcc attccttctt ttctgtaagt attgcggagc ccgtctgctg
      301 cagcagctct gatgaaatag tttggatgac ccagcgcaaa tgtcagagtg tcaataccca
      361 ctttgattga atcgagaatc ggagtcttct tcttgtcttt tttctcgtct gcagattcct
      421 ctttcacatc catattatca atcagtgtac cgatagaacg cagaacctca gctatgccta
      481 caacaagacg catattttcg tctttttcat aagtaggctt atcattttct ttgattctgt
      541 cctgccactg atagaataca tccactaaag gctgcattcc tctggtatgt cctaaatcag
      601 caattgcctg agaagcaaga tatttcagaa ttggagcatt gtgttcagca atattcgcat
      661 cgagcggaat gactgtctcc ggattcaaaa tttgacggga agctttttcg ataacttccg
      721 gtttgtcata aaaaccgaat ccgccgtctt ttacttctga aggagtcaac attttctctt
      781 tcattttgtc attttctttc aaagaaacca ggtcggcatc aatttccaca cttcccttga
      841 ctgctaaaat caggggacgg agggctctta atgtacggtg ttttttaaga gttccgatag
      901 catctattct ctcttttaaa ctacccttgc ctaatttatc cacctgagat tgaaagaaag
      961 catcgcttcc ttccttatag gaatcaattt cttctgccag taccgaaaat gccagtatta
     1021 cagagaatat taaaaatttt ttcatgttct atgctccttt gattgaggtg cctgcttttc
     1081 ccaaaagagt gtttgccttt ccagaagaaa aattcaggca gtgtttgctg actaaagtat
     1141 agatgatttt agggggggat aaaaatccaa gcatttttac agcgttcgga cagaattctc
     1201 agatttccta aaaaaaacag tttttcgaat atcggcttga atttattctg tcaaaaggct
     1261 atgaatgacc agaagacaga tacagtaatc ggcgaagaca ttgtttttaa aggtacagtt
     1321 cggtttcaga actgtctgag agttaaggga ttcatcaaag gctctgttca ggctgagggc
     1381 gatttagtgg tggaagaagg cggaaaagtg gaagctgacg tcaactccaa cagtgcag
//
LOCUS       DAOQGJ010001280         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69680_length_1437_cov_3.317656, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001280 DAOQGJ010000000
VERSION     DAOQGJ010001280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69680_length_1437_cov_3.317656"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1120)
                     /locus_tag="PK683_17150"
     CDS             complement(29..1120)
                     /locus_tag="PK683_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH10229.1"
                     /translation="MNITKIMVLAYSVFFLQNCFTTAPVKETTLGEKLNERISNPSDT
                     TQTLKVYFETNRKILSPNPSCSNEHYGVQIDGTERYGACAVSVPAEHEIGNLFLEPSK
                     GKEVSFILESHSNMDKDVFFNSIKAETFPEVLVFVHGFNVKFEEAVLRAAQIKYDLKF
                     AGPVVLFTWPAGSEDGFLSKIRIDDTYKNNFANAMGSGEHFKEFLSRISNLGKVAHVI
                     VHSMGHQVVLRGIDKVYSQKKERFIKELVLNAPDFESVEFANIAPNLKASAERITVYC
                     SPGDNALVASSKVNSNKRVGSCEKIPGVDMINVNQVDAPVFGLGHGYYSSRPVLTDLF
                     QTILGIEARRRLFIRKSGPYNSEDYVLRK"
     gene            complement(1123..>1437)
                     /locus_tag="PK683_17155"
     CDS             complement(1123..>1437)
                     /locus_tag="PK683_17155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH10230.1"
                     /translation="FHEAWDIQANYEPVYAVKSGKVIYSGWMGGYGNAVILEHTKELK
                     SLYAHNSRIIVKEGEYIQGGKQISVSGCTGYCFGAHLHLEIIKNGVSVNPKDYIKGYY
                     PR"
BASE COUNT          381 a          354 c          275 g          427 t
ORIGIN      
        1 gatcatgaat ttttacaact ccgctcaact actttcgtaa aacgtaatcc tctgaattgt
       61 acggccctga ttttcggatg aacagtcttc ttctggcctc tatccctaga attgtctgga
      121 aaagatcggt gagaacaggt ctggaagaat aatatccgtg accgagtccg aacacaggcg
      181 catccacctg attcacattg atcatatcca ctcctggaat cttctcgcag gagcccactc
      241 tcttattgct gttcactttg cttgatgcaa caagggcatt gtctcctgga gaacagtaca
      301 cagtaatcct ttccgcactt gccttcagat taggtgcaat atttgcaaat tccacagatt
      361 caaaatcagg tgcattcaga acaagctcct tgatgaatct ttccttcttc tgagaatata
      421 ccttgtctat cccccggagc acaacttggt gtcccatgga atgcacaatc acatgagcca
      481 ccttgcccag attggaaatt cttgaaagaa attccttaaa gtgttctcca gaacccatcg
      541 catttgcaaa attattcttg tatgtatcgt ctatacggat cttgctcaga aagccgtctt
      601 cagaccctgc cggccaggta aaaagaacaa caggtcctgc aaacttcaga tcgtatttga
      661 tctgggctgc ccgaagcact gcttcctcaa attttacatt gaacccgtgt acaaatacaa
      721 gaacttccgg aaaagtttcc gcctttatgg aattgaaaaa aacatcctta tccatattgg
      781 aatggctttc caggatgaag gatacttcct ttcccttgga cggttcaaga aaaagatttc
      841 ctatttcatg ctctgcaggg acactgacag cgcaggcacc gtaacgttca gtaccatcta
      901 tctgaacacc ataatgttca ttggaacagg aaggattcgg actgagaatt tttctgtttg
      961 tttcaaagta cactttcagt gtctgagttg tatcggaagg attggaaatc ctctcattca
     1021 gcttttcacc gagtgtagtt tccttgaccg gtgccgtagt aaaacagttc tgcagaaaaa
     1081 atacagaata tgccaatacc attatttttg tgatgttcat atttacctcg ggtaatatcc
     1141 ttttatgtag tccttgggat tcacggaaac cccgttcttt atgatttcca ggtgcaggtg
     1201 ggctccgaaa cagtatcctg tgcatccgga tactgaaatc tgttttccgc cctgaatgta
     1261 ttctccctct ttcacgatga tcctggaatt gtgcgcatag agggacttca gctccttcgt
     1321 gtgctcaaga atcactgcat ttccataccc ccccatccag ccgctgtaaa tcacctttcc
     1381 gcttttgact gcatatacag gttcataatt cgcctgaatg tcccaggcct catgaaa
//
LOCUS       DAOQGJ010001281         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69703_length_1437_cov_2.712735, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001281 DAOQGJ010000000
VERSION     DAOQGJ010001281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69703_length_1437_cov_2.712735"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PK683_17160"
     CDS             complement(<1..389)
                     /locus_tag="PK683_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNH10231.1"
                     /translation="MKIFFIIIAVLISCSRQEEKKEAAVQPEKPAEQKMKRLIFFGDS
                     LTAGYGLLPEENYVHLISEKLKQEGFNLEVVNAGVSGDTTATGLARLDWVIKKGADYF
                     VLELGANDSMKGISISEIRSNLKKIIS"
     gene            complement(400..>939)
                     /locus_tag="PK683_17165"
     CDS             complement(400..>939)
                     /locus_tag="PK683_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH10232.1"
                     /translation="QNGNKSILQMNDSIFNINESSKKMLGIIEIINSIASQTNLLALN
                     ASIEAARAGDAGLGFSVVASEISKLAEKSTGSTKNIQELIRMTNEEAGQGKINIEKGG
                     ENFRNIIAEVEKIHRKISDVNDQMSDSAEKYNDFQDRIAELDKEADSIHTKVTNQVDQ
                     LKTIMSSMIQVEEDFRKLK"
     assembly_gap    940..949
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<950..>1437)
                     /locus_tag="PK683_17170"
     CDS             complement(<950..>1437)
                     /locus_tag="PK683_17170"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH10233.1"
                     /translation="NNEEIIGKTDFDITPDKALVEQFLASDLEVIKNRKEKVIRRDKI
                     LNNKGDICYVDTIKSPIINEDGSCDHMLGISLDITEKTRMEMVLEESRLKLSDLVQKV
                     LASMKNILQVSDEIKEVVQVQTLDIKAVDQITAHILEANRLTGSMLSEILRLTSHTVG
                     LA"
BASE COUNT          353 a          337 c          247 g          490 t
ORIGIN      
        1 ctgctgatta tttttttaag attggaacga atctcggaaa tactgatccc tttcatggaa
       61 tcatttgcac cgagctccag aacaaaataa tctgcgcctt ttttaatcac ccagtcaagt
      121 ctcgcaagtc ccgtggccgt tgtatctccg ctcacgcctg cattgacaac ttccagattg
      181 aatccttcct gtttcagttt ttctgaaatc agatgaacat aattttcttc aggaagaagt
      241 ccgtatcctg ctgtaagact gtcaccgaaa aatatcagtc tcttcatttt ctgttctgca
      301 ggtttttccg gctgaacagc tgcttctttt ttttcttcct ggcgactgca ggatatcaac
      361 actgctatta taatgaaaaa tatcttcaaa acatacctcc tatttgagct ttctaaaatc
      421 ttcctctacc tgaatcattg aactcatgat cgttttcagc tgatccacct gattggtaac
      481 cttagtatga atagaatctg cttctttatc aagttcggca atcctgtcct gaaaatcatt
      541 gtatttctct gcactgtcac tcatctgatc attcacgtca ctgatttttc tgtgaatttt
      601 ttccacttcg gcaattatat ttctgaaatt ctctccgccc ttttcaatat tgatcttacc
      661 ctgaccggct tcttcgtttg tcatcctgat cagctcctga atgtttttgg tgcttccagt
      721 ggacttttca gcaagcttgg aaatctcaga agcaacaaca gaaaacccca gtcctgcatc
      781 ccccgccctg gcagcttcaa tggatgcatt gagtgcgagc agatttgtct gcgaggcaat
      841 gctgtttatg atttcgataa tcccaagcat ttttttggaa ctttcattaa tattaaatat
      901 agaatcattc atctgcagaa tcgatttgtt cccgttctgn nnnnnnnnna gccagtccca
      961 ctgtatgact tgtcagccga agaatttcag aaagcatgga tccggtaagc ctgtttgcct
     1021 caaggatatg ggctgtaatc tgatctacag cctttatgtc aagtgtctgc acctgaacaa
     1081 cttccttgat ttcatcggat acctgaagta tgttcttcat tgaagcaagc accttttgaa
     1141 ccaggtcgga aagcttgagc ctggactctt ccagtaccat ttccattcta gtcttttctg
     1201 ttatgtccag agaaataccc agcatatggt cgcaggatcc gtcctcattg ataataggag
     1261 acttgattgt atcgacataa catatatctc ctttattatt caggatttta tcccgacgga
     1321 tgactttctc tttccggttt tttatgactt ccagatccga tgcaagaaac tgctccacca
     1381 gtgctttgtc gggagtaatg tcaaaatctg tttttccaat gatttcttca ttattta
//
LOCUS       DAOQGJ010001282         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69783_length_1436_cov_3.042723, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001282 DAOQGJ010000000
VERSION     DAOQGJ010001282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69783_length_1436_cov_3.042723"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK683_17175"
     CDS             <1..492
                     /locus_tag="PK683_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10234.1"
                     /translation="RTFRLILKNADNGTVYLKYIIGTSYKILKITENLVWIFQKGLHG
                     TEGIICLEMRFGLKVLDKTDRSSAVFPLKSEDGNIWTVEWDAKKNKTGSGLQISAYNP
                     STAKPIDIQKVLSAKYPSVNSPVKSFRFIQDKSSAGGDSDLYELSGIQVRSENGNEFT
                     IQL"
     gene            651..977
                     /locus_tag="PK683_17180"
     CDS             651..977
                     /locus_tag="PK683_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10235.1"
                     /translation="MSDFIANFRKFDSMKETTTENSGIYLEEAMNRIPEYYDRDIIKI
                     LLKNPKEAFVFWGISLDSYKKIEDYFGKSRNEIRYKLFVRYLDESKTPHFQEILLAPY
                     AQSYVI"
     gene            1047..1346
                     /locus_tag="PK683_17185"
     CDS             1047..1346
                     /locus_tag="PK683_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10236.1"
                     /translation="MMHSAAIHLPRNRPSHLFHKDWLDPRFKAYFTAVSPDLYTDIAG
                     LAIPGAAEKLGTLDFSKLAEKTEAVVQTSVTKYYNVNSESRPYNINSEFMNGGNS"
BASE COUNT          467 a          282 c          295 g          392 t
ORIGIN      
        1 agaacattca gactgatact gaaaaatgca gataacggca cagtatatct gaaatatata
       61 ataggaacct cctataaaat actgaaaatc acggaaaatc tggtctggat tttccagaaa
      121 ggactgcacg ggacagaagg aataatatgt ctggagatga gattcggtct gaaggttctc
      181 gataaaacgg acagaagttc tgcagtgttc cccctgaaat ccgaggacgg aaatatttgg
      241 acagtggaat gggatgcaaa aaaaaataaa accggatccg gactgcagat cagtgcgtac
      301 aatccttcca cggccaaacc cattgatatt cagaaggttc tgtctgcaaa atacccatcc
      361 gtcaattcac cggtaaaatc attccgattc atacaggata aaagcagtgc aggcggcgat
      421 tctgatcttt atgaactcag cggaatacag gtgcgatctg aaaatgggaa tgaatttaca
      481 attcaactgt aaaaaatcgt ctaatgtcag ggaaaggaaa gtttttacag tacagctttt
      541 actgctatta gacacagggc ttctgttttt taccgcaaaa ccctcttcca aattttgccc
      601 gacttgagtg agaattgtca tgagttagtt tgagttcgtc gtacgattta atgagtgact
      661 ttattgccaa ttttagaaag tttgacagta tgaaggaaac aactactgaa aattcaggga
      721 tttatcttga agaagcgatg aacaggatac cagaatacta cgaccgggat atcatcaaga
      781 ttcttttaaa gaatccgaaa gaggcctttg tattctgggg aatcagtctc gattcttaca
      841 aaaaaataga agattatttc gggaaatcca gaaatgaaat ccggtacaag ctatttgtac
      901 gatacttgga tgagtctaaa actccccatt ttcaggaaat ccttctggca ccttatgcac
      961 agtcctatgt catctagttc ctgggaccag taaaaaatct tcgagcagag atcagtgctt
     1021 acgttgaaca ggaaggtgag tacactatga tgcattctgc tgctatccat cttcctagaa
     1081 acagacctag tcatctgttt cacaaggact ggctggatcc aagattcaag gcatatttta
     1141 ccgctgtttc tcctgatctg tacaccgata ttgcaggact tgcaatcccc ggtgctgcgg
     1201 aaaaattagg aacattagat ttttctaaac ttgcagaaaa gactgaagca gtggtacaga
     1261 catctgtaac aaagtactat aatgtaaatt cagaatcccg gccttacaat atcaattctg
     1321 aatttatgaa cgggggtaat tcataatgct tcaaggatac gaacttttag tactgcacgc
     1381 gcaccttcct tatgtgcgcc atccaaatta ttatcctccg ttcattgagg aaaact
//
LOCUS       DAOQGJ010001283         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_69949_length_1434_cov_3.056563, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001283 DAOQGJ010000000
VERSION     DAOQGJ010001283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69949_length_1434_cov_3.056563"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PK683_17190"
     CDS             <1..551
                     /locus_tag="PK683_17190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH10237.1"
                     /translation="AEYIKSIFKLQDTEIHSLGDFGFGLGALLQETSLTLNVSRIVAL
                     DISSDCVNRLMRQSWHSKKDIAILNASIEDYESPYLEKHPLDLIIFNSVMQYIPDRSV
                     KSVFKKLSKYCKYLYFCAPTEKDYDLMKEVMDFTDPYAYSRPLSFYRKSWKPFFTAVG
                     LNLLESRSNVKDSPFVFELYRY"
     gene            553..>1434
                     /locus_tag="PK683_17195"
     CDS             553..>1434
                     /locus_tag="PK683_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10238.1"
                     /translation="MKIVLCTDNSTLLDSISDHLKKITCSPDFTLLKTQVLILCRETF
                     SEDYLYMKLSERGIPSLAYEIRSMKDFMRDVLEKTDPKTETAGRLEELFLLQKVISEN
                     GKFKERNLPLSALFHIASDIYDIFEKHRKSGKAIPENWKYADIWKEYRKKKTKLSPSE
                     KMLKFLDSASRKKNPADLLSEYTHLFLAGHYHIDPVQKKFAEHLSSLMKAKNSEPRAF
                     ICRSRNILIDPEDERNSETGYLLKEFPGAETEFFGKDKSFRAFFFSAPEIFRELETVG
                     RRILKILHEKRNIPSFRL"
BASE COUNT          464 a          294 c          283 g          393 t
ORIGIN      
        1 atgcggaata tattaaaagc atttttaagc ttcaggatac tgaaatccat tcccttgggg
       61 actttggatt cggcctgggt gcacttcttc aggaaacctc tctcacactg aatgtaagca
      121 ggattgtggc attggatatt tcatccgact gcgtaaacag actgatgagg cagtcctggc
      181 acagcaaaaa agacattgct atactgaatg cctcgataga ggactatgaa agcccttatc
      241 tcgaaaaaca tcctctggat ctgattattt tcaattcagt aatgcagtat attcctgaca
      301 gatcagtaaa atctgtattt aaaaaacttt cgaaatactg taagtatctt tatttctgtg
      361 ctccgaccga aaaagactac gatctgatga aggaagtaat ggattttacg gatccgtatg
      421 catactcccg gcccttgagt ttttacagga aaagctggaa gccttttttc acggcggtcg
      481 gactgaatct tctggaaagc agatcaaatg taaaagactc gcctttcgtt ttcgaacttt
      541 acagatactg aaatgaaaat tgttctctgc acagacaaca gtacccttct ggattcaatt
      601 tcagatcatc tgaaaaaaat tacctgcagt cctgatttta cccttctgaa aacacaggtt
      661 ctcattctgt gccgtgaaac tttctctgaa gattacctgt atatgaagct ttcagaacgc
      721 ggaatcccct cccttgccta cgaaataaga tctatgaagg attttatgcg ggatgttctg
      781 gaaaaaaccg accctaagac ggaaacagcg ggaaggcttg aagaactttt tctgctgcag
      841 aaagtaattt cggaaaatgg aaaatttaaa gaaagaaatc tcccgctttc tgcattattt
      901 catatagcat ctgacatata tgatattttt gaaaagcacc gcaagtcagg taaggcaatt
      961 cctgaaaact ggaaatatgc agacatatgg aaggaataca gaaaaaaaaa gacaaaactt
     1021 tctccctccg aaaaaatgct gaaatttctg gattcagcct cacggaaaaa gaatcctgcc
     1081 gacttgctct ctgagtacac tcatcttttt cttgcaggac actatcatat agaccctgtt
     1141 cagaaaaaat tcgctgaaca tctcagctcc ctgatgaaag ccaagaattc agaaccgcgt
     1201 gcattcatct gcagaagcag aaatattctg attgatccgg aagatgaaag gaattcagaa
     1261 acaggctatc ttttgaagga atttcccgga gcagagacag aattcttcgg aaaggacaaa
     1321 agcttccgtg ccttcttctt ttcagcaccg gagatattca gagagctgga aactgtaggc
     1381 agaaggattc tgaaaatact tcatgaaaaa agaaatattc cgtcattcag actg
//
LOCUS       DAOQGJ010001284         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70134_length_1432_cov_2.783588, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001284 DAOQGJ010000000
VERSION     DAOQGJ010001284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70134_length_1432_cov_2.783588"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PK683_17200"
     CDS             complement(<1..1394)
                     /locus_tag="PK683_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNH10239.1"
                     /translation="MQRKTGIRDSAALALSDWTATAEYSEDAVWPRKWAEYYVNSSRE
                     RIYDWLISHGQKFFPIVHWAERGLFRPGNSVPRFHMLIGLGAGAAKSLSEQLLFHRNR
                     NRLAVLFRHNVTKLIIGKNTVGCTGLNEENEELFSAEAENLIIASGGLNGDLNRVRKN
                     WDRESFGEPPEVILNGSHRYADGAVHDLIAGLGGNVTFEQNMWNYPSGVHHPEPDKEN
                     HGLTIVPPKSALWMNYEGRRIGPMPLVTAYDTRYIVERICQEKRKYSWQILNWKIAVK
                     ELAVQNSDYNEAMRDKKTLKFIFNVIFGNEKLVKRMTETCKDFVTADTVQELAEKMNA
                     LNGDNAVNASLLEEEIRKYDAGILRGEIFHNDEQLRRIAHARQYRGDRLRTCRFQPIL
                     DRKAGPLFAIREFINSRKSLGGIQTDLSSRVLTSSGAVIPGVYAVGECAGFGGGGIHG
                     KGALEGTFLGSCILT"
BASE COUNT          343 a          370 c          298 g          421 t
ORIGIN      
        1 gtcagaatgc aggaacccaa gaatgttcct tccaatgccc cttttccatg tatgcctcct
       61 ccgccgaaac ctgcacattc cccgacagca tatacaccgg gaatgactgc accggatgaa
      121 gtcagtacac ggctggaaag atccgtctgt attccgccta gacttttcct ggaattgatg
      181 aattccctga ttgcaaacag gggccctgcc ttcctgtcca ggatcggctg aaacctgcag
      241 gtgcggagtc tgtctcctct gtactgcctg gcatgtgcaa tacgacgcag ctgttcatca
      301 ttatgaaata tctcacctcg cagaatgccc gcatcgtatt tccggatttc ctcttcgagg
      361 agtgaagcat tgaccgcatt atctccgttc agtgcattca ttttttctgc gagttcctga
      421 acagtgtccg cagtcacaaa atccttgcag gtctctgtca ttctttttac aagtttttcg
      481 tttccgaata tcacattgaa gataaatttt aatgtttttt tatcccgcat tgcttcattg
      541 tagtcggaat tctgaacggc aagttctttg actgcaattt tccagttgag aatctgccag
      601 gagtattttc ttttttcctg acagatcctt tcgacgatat atctcgtatc gtaggctgtg
      661 acaagaggca tcgggccgat tcttcgccct tcataattca tccagagagc agatttagga
      721 gggacaattg tcagtccgtg attttccttg tcaggttcag gatgatgcac accggaagga
      781 tagttccaca tattctgttc aaacgttaca tttcctccga gacctgcaat cagatcatgc
      841 actgcaccgt ctgcatacct gtgagaaccg ttcagtatca cttcaggagg ttcaccgaaa
      901 ctttccctgt cccagttttt tctcacacgg ttcagatctc cgttcagtcc tcctgaagca
      961 atgatcagat tttcagcttc tgcagaaaat aattcctcat tctcttcatt gagtcccgtg
     1021 catccgactg tattttttcc gataataagt tttgttacat tgtgacggaa aagaacagcc
     1081 agcctgttcc tgtttctgtg aaaaagaagc tgttcagaga gactctttgc agcccctgcc
     1141 ccgagtccga tgagcatatg aaacctggga acggaatttc cgggacgaaa aagtcctctc
     1201 tccgcccaat gaactatcgg gaaaaatttc tgtccgtgag aaataagcca gtcatagatc
     1261 cgttctctcg aactgtttac ataatattcc gcccatttcc tgggccagac cgcatcctct
     1321 gaatattctg ctgtagcagt ccagtcgctg agtgcaagtg cggcagaatc ccggatgccg
     1381 gtctttctct gcaccaatga actatcggga aaaatttctg tccgcgagaa ag
//
LOCUS       DAOQGJ010001285         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70135_length_1432_cov_2.771968, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001285 DAOQGJ010000000
VERSION     DAOQGJ010001285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70135_length_1432_cov_2.771968"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PK683_17205"
     CDS             complement(<1..604)
                     /locus_tag="PK683_17205"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10240.1"
                     /translation="MLKSFFLLLVSMYSLYAECISGDCENAKGVYVFENGTKYEGDFK
                     NSAPSGLGVMFFKKNKIYLGQFENGIPEGKGSMFQSGGKKSYYGEFHKGRKEGLGVMD
                     YGNSMYYEGYWLNGKRDGKGTLVSKDRSYLYTGDWVKGKQTGNGKLNFSKDGVDAVYQ
                     GGFQKGVFNGYGTLAFKDGNKYTGEFQEGRFHGKGTLTNAK"
     gene            complement(604..1431)
                     /locus_tag="PK683_17210"
     CDS             complement(604..1431)
                     /locus_tag="PK683_17210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH10241.1"
                     /translation="MKKKSSRDEEFTVVKCRNCGLVQVNPQPDFADVAKYYSNEYFTQ
                     RTDRGYDNYYSDKIRSEIERVFQLNLRDLKFNEWEEKLPEKGRTIDIGCAAGYFVKFM
                     EGKGWNSEGIEIAEGPVKFGREALGLTIHQEDFLSWDENFENKFDLVTLWASIEHLHR
                     PRNTVEKIYRHLKPGGRMILSTCRYGTLAKLKGAGWRFLNVPEHLYYYSLEGLRKLSE
                     NAGFKYVSHISYGSGMTAKKNSGAVYNIMKKILDKAVKIFDEGDMMALQFEKPPEAS"
BASE COUNT          354 a          379 c          241 g          458 t
ORIGIN      
        1 ctttcgcatt tgtcagagtt cctttcccgt gaaaccgtcc ttcctgaaac tcccctgtat
       61 acttgtttcc atccttgaat gcaagagtgc cgtaaccgtt gaacacccct ttctggaatc
      121 cgccctgata gactgcatct actccgtctt tgctgaaatt cagcttgccg tttccagtct
      181 gctttccttt gacccagtct cctgtataca gataggatct gtcttttgat acaagtgttc
      241 cttttccgtc cctttttccg ttcagccagt aaccttcata gtacatactg ttcccatagt
      301 ccatgactcc cagtccttcc tttcggccct tgtggaattc cccatagtag gatttttttc
      361 cgcctgactg aaacatggaa cccttgcctt caggaatgcc gttttcaaac tgtccgaggt
      421 agatcttgtt ttttttgaag aacattacac cgagaccgct gggagcggaa tttttaaaat
      481 caccctcgta ctttgtccca ttctcaaaca catagacacc ctttgcattt tcacagtctc
      541 ctgaaatgca ttccgcataa agagaataca ttgaaaccaa aagaaggaaa aaacttttca
      601 gcatcaggaa gcctccggcg gtttttcaaa ttgcagagcc atcatatctc cttcgtcaaa
      661 aatcttgaca gccttgtcaa gtattttttt cattatattg tacactgcac ctgaattctt
      721 ttttgcagtc atcccgcttc cgtaggaaat atgagagaca tacttgaatc cggcattctc
      781 agaaagcttt ctgagtcctt ccagactgta atagtacaga tgttcaggaa cattcaggaa
      841 tctccagcct gcacctttca gcttcgcaag tgttccgtat ctgcatgtag aaagaatcat
      901 tctgccgccc ggcttcagat gtctataaat tttttccact gtattcctag gtctgtgcag
      961 atgctcgata gatgcccata atgtgaccag atcaaacttg ttttcaaaat tctcatccca
     1021 ggaaagaaaa tcttcctgat gaattgtaag cccaagtgct tcccgtccaa atttgacggg
     1081 accttccgca atctcaatgc cttccgaatt ccatcccttg ccctccataa atttcacgaa
     1141 gtatccggcg gcacatccaa tgtctatcgt ccttcctttt tcaggaagtt tctcttccca
     1201 ttcattgaat ttcagatctc tcagattcag ctgaaacacc ctttcaattt cagaccgaat
     1261 tttatcggaa taataattat cataacctct gtccgttctt tgagtaaaat actcattgga
     1321 atagtatttg gcaacgtctg caaagtccgg ctgaggattg acctgaacaa gcccgcagtt
     1381 tctgcacttc actacagtaa actcttcatc cctgctgctc ttttttttca gg
//
LOCUS       DAOQGJ010001286         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70199_length_1431_cov_4.345203, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001286 DAOQGJ010000000
VERSION     DAOQGJ010001286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70199_length_1431_cov_4.345203"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>1431
                     /locus_tag="PK683_17215"
     CDS             112..>1431
                     /locus_tag="PK683_17215"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH10242.1"
                     /translation="MKIRDKLFIAFAGVIAVMTVCFVYIFIQFGSIRKSLDGITHDRT
                     PKVITSYELLRHYNQVARSVRNLLILDSPEELAKEMQTITELMDIEIPKQIEYFRTRS
                     NTEKGKELVEVLAEKKALMSIQVNLFLDLIRKGKKDEARLVLLSKAPNSMGQLAFTKN
                     IGNYADYQRSLLKDQSDNIYSVLENSYIVFTSSFVCALFFSLFIAYFLIKNITQPMRR
                     MTSAVDTLGSGNLNVDFEDADRKDELGQLSRSFQAMTKKLQMLIAEVGRFNNSASVGD
                     FSYNSDESKYFGDFSAIKIGSDRLKENLINLINSMNEMSDQHTRGDIDVKIDSGKFQG
                     SFREMAEKINTMVFDHIKVKKSAVGVFREFGEGNFEANMEILPGKKRFINETIDQVRS
                     NLKLVISEINSLSASAIEGKLSVRADVSSHKGDFRKIIEGVNQTLDSV"
BASE COUNT          465 a          261 c          315 g          390 t
ORIGIN      
        1 atcattttta cagtatgcgg actttgaaag ggatatgaac ggagaatgtt tctcaaattc
       61 tccttcgtgc agtcaaattc tgacctcaaa atataaaggc aggaaaaaat aatgaagatt
      121 agggacaagc tgtttattgc atttgccggt gtcatagctg tcatgacagt atgttttgta
      181 tatattttta tacaattcgg ttcaatcagg aaatcactgg acggcatcac tcatgaccgc
      241 actccgaagg taattaccag ttacgaactt ctacgacact acaatcaggt cgccagatca
      301 gtcaggaacc tcctgattct ggacagtccg gaggaacttg ccaaggaaat gcagacaata
      361 actgaactta tggatatcga aattccaaag cagatagagt atttcagaac aagatcaaac
      421 acggaaaaag gaaaggagct tgtcgaagtt ctggcagaaa agaaagcact gatgtccata
      481 caggtcaacc tgtttctgga tctgatcaga aagggcaaaa aggatgaagc aaggcttgtc
      541 cttctgtcga aagcgccaaa ctcaatggga cagcttgcat tcacaaaaaa tataggaaat
      601 tatgccgatt atcagagatc tcttctgaaa gaccagtcgg acaatattta cagtgttctg
      661 gaaaattcat acattgtctt cacctcatca ttcgtatgtg cacttttttt cagtctgttt
      721 attgcatatt tcctgattaa aaatattact cagccgatga gaaggatgac ttctgcagtg
      781 gatactctcg gatccgggaa tctgaatgtt gattttgaag atgccgaccg gaaagacgag
      841 cttggtcagc tgagccggtc atttcaggca atgacaaaaa aacttcagat gctgattgct
      901 gaagtaggca gattcaataa ttcggcttct gtgggtgatt tctcttataa ttcggacgaa
      961 tcgaaatact ttggagattt ttctgcaatc aaaatcggca gtgacaggct gaaggagaat
     1021 ctgatcaatc tgatcaattc catgaatgaa atgtcggacc agcatacccg gggggacatt
     1081 gatgtaaaga ttgattcagg taaattccag ggctcttttc gggaaatggc tgaaaaaata
     1141 aatactatgg ttttcgatca tataaaagta aaaaaatcag ctgtcggtgt attcagggag
     1201 tttggagaag gcaactttga agcgaacatg gaaatacttc ctggaaaaaa aagattcatc
     1261 aatgagacaa tcgatcaggt cagatccaat ctgaaacttg tcatttctga aatcaattct
     1321 ctctctgcgt ctgcaattga aggcaaactt tcagtaagag cagatgtttc cagtcataag
     1381 ggggatttca gaaaaatcat tgaaggagtc aatcagacgc tggattcagt g
//
LOCUS       DAOQGJ010001287         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70219_length_1431_cov_3.263808, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001287 DAOQGJ010000000
VERSION     DAOQGJ010001287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70219_length_1431_cov_3.263808"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PK683_17220"
     CDS             <1..871
                     /locus_tag="PK683_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10243.1"
                     /translation="TANQPGLVNSFARRLVEDHPDKKIIELEDYLRVEEWYLTEAIDK
                     NVSNIINKASKYRSFVEGLLFTELTVPFAIDRPSIKELFVNGIIKKGKDGNVEFWVPL
                     YKKRLFSAFFPYTNGEGKRIAGNMFSSEYLTSDNKINFNKLIDSYRDHIRLRSFRPFR
                     EKDENGNYLSIPEAAMIYSFETYISIFLRELDAKVYREAFVSLGNTDLIINVHGFEYL
                     LEIKKYYSPSYFVKGKKQLAYYCNRAGLNTGYYIVFIENTINPNYVSDLTEIIDGVEI
                     ITFLIWYDEEKDF"
     gene            complement(940..>1431)
                     /locus_tag="PK683_17225"
     CDS             complement(940..>1431)
                     /locus_tag="PK683_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10244.1"
                     /translation="AANQDMNGIILSGFLNQAKNNLNGSAIGLVNIVKKDLTGIAVSV
                     FGNAVENSLKGIAISGTVNEAKNISGFSISLSNKAYNDFTGISIGLINSCKNNMSGIQ
                     FGGYNYSNSSAGIQAGLVNYAKTSSFLQLGLLNISENSLLPIFPIINLNLSALFKDET
                     IDF"
BASE COUNT          439 a          272 c          301 g          419 t
ORIGIN      
        1 cactgccaat cagccgggtc tagtcaacag ttttgcccgc agacttgtgg aagatcatcc
       61 cgataaaaaa atcatagaac ttgaagatta tctgagagtg gaggaatggt atctcacgga
      121 ggctatcgac aaaaatgtat cgaatatcat aaataaagcg agtaaataca gaagtttcgt
      181 ggaaggtctg ctgttcacgg agctgactgt tccatttgca atcgacagac cgtccatcaa
      241 agaactgttt gtgaacggca ttattaaaaa agggaaagac ggaaatgtgg aattctgggt
      301 cccgttgtac aagaaaagac tgttcagtgc ctttttcccc tacacaaacg gagagggtaa
      361 aaggatcgca gggaatatgt tttcctccga atatttgact tctgataata aaatcaattt
      421 taataagttg atagacagtt acagagatca tattcggctc aggagctttc ggcctttccg
      481 ggaaaaggac gaaaacggaa attatctctc gattccggaa gctgcaatga tttacagttt
      541 cgaaacatac atcagtattt tcctccggga gctggatgcg aaagtgtaca gagaagcctt
      601 tgtttctctg gggaatactg atcttatcat caatgttcac ggattcgaat atcttctgga
      661 aattaaaaaa tactacagcc cttcctattt cgtcaaagga aaaaaacagc tggcctatta
      721 ctgtaaccgt gccggcttga atacaggata ttatattgta tttatagaaa ataccatcaa
      781 tcccaattac gtatccgacc tgactgagat cattgacggg gtggagatca tcacttttct
      841 catctggtat gatgaagaaa aggatttttg aaatgaatgg ggggcagaga ccacggtgaa
      901 caaaaacggt atgctgtgtt gatcagcaag gttgccgtat cagaaatcaa tcgtctcatc
      961 tttaaaaagc gcagacagat tcagattaat aattggaaaa atcggcagca gactgttttc
     1021 tgaaatattc agaagtccca attgcagaaa ggatgatgtt tttgcatagt tgacaagacc
     1081 agcttgaatc cctgcgcttg agtttgaata attataaccc ccaaactgta taccggacat
     1141 attattttta cagctgttga taagtccaat ggagatgccg gtgaagtcgt tatatgcctt
     1201 attggaaagt gaaatcgaaa agccgcttat attttttgct tcatttacgg ttccgctgat
     1261 tgcaattcct tttaagctgt tttccactgc atttccaaat acgctcactg caattccggt
     1321 taaatccttt tttactatgt ttacaagtcc tattgcggat ccatttaaat tgttttttgc
     1381 ctggttcaaa aatccgctta aaataatgcc gttcatatcc tggttggcgg c
//
LOCUS       DAOQGJ010001288         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70299_length_1430_cov_3.491636, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001288 DAOQGJ010000000
VERSION     DAOQGJ010001288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70299_length_1430_cov_3.491636"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(428..>1430)
                     /locus_tag="PK683_17230"
     CDS             complement(428..>1430)
                     /locus_tag="PK683_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10245.1"
                     /translation="LRDVAEGQEGLGIKIITVQIREAVVSSSSLWNDLQSPFRNEQKK
                     KARISQLEMENEIHTKELETRKLKENREADTNLEIEQKKQQKLTEVIDLKLKEELKRF
                     EEEQKSKQTKQSLEEETIMRKKETDDRIATKEKEILLRQSLEEMKRRDAQIAEETKIQ
                     LEAEKRRLAQESEQKLFEMKEQEKQLKSEHEVYLQKLDREKLTAEKEFSMDQIRKNFE
                     YEIEQLRVRAELRVQEVQKEMELNLRKKENEIQLRYKEEKIRLERFMMETENLMNGNH
                     LTSKMIAALPEIAAQMPEIKELKILQSDGSDPMFDGLTVFIQKILSLSENFSLRRKK"
BASE COUNT          332 a          330 c          273 g          495 t
ORIGIN      
        1 tgcttttaca caacctcaat ccccgcattt actcacatac agtgtcaatt ggaattgact
       61 ttaagcggta tctcaatgat cctattatga tgtaattaga aatctgagaa ctaaggtcgg
      121 gtatgcgtgc tgctcctctt caggtgcgag gactccaaat ccgtcagagt cttccagcgt
      181 cgaaagctca attgctttta ggggcaggca aaaggaggca tatactggtt ttcaaaaata
      241 attgcgtttt ttcatactgg caggcttaat tttattgagt cgagacagaa taaatgcgca
      301 aaaactcttg ggaattagca taagtccggc gttaagaaag ctatattgaa aataaatatt
      361 taatataatg ataaattgtc agggagaagg agggactgcc tccctgaatg aggaggcagt
      421 ctgaaaatta tttttttctt ctgagactga agttttcaga gagacttaag atcttctgaa
      481 tgaaaacagt cagaccgtca aacataggat cagaaccgtc agactggagt attttcagct
      541 ccttgatttc aggcatctgt gccgcaattt caggaagagc tgcgatcatc tttgaagtca
      601 aatggtttcc gttcatcagg ttttcagttt ccatcataaa tctttccaga cggattttct
      661 cttccttgta acggagctgg atttcatttt cttttttcct cagattcagc tccatttctt
      721 tctgcacctc ctgtaccctc agttccgcac ggaccctgag ctgctcaatt tcatactcaa
      781 aattctttct gatttgatcc atactgaatt ctttttctgc agtgagcttc tctctgtcga
      841 gtttctgaag gtaaacttca tgctccgact tcagctgttt ttcctgctct ttcatttcaa
      901 agagcttctg ttccgattcc tgagcaagcc tcctcttttc ggcttccagc tgaatctttg
      961 tttcttccgc aatctgagca tcccttcgtt tcatttcctc aagagactga cgaagaagta
     1021 tttccttttc cttggttgcg atcctgtcgt ccgtttcttt cttgcgcata atcgtttctt
     1081 cttccagact ctgcttagtc tgcttgcttt tctgctcttc ttcgaaacgt ttcaattctt
     1141 ccttgagttt gagatcaatg acttcagtga gtttctgctg ctttttctgt tcaatttcaa
     1201 gatttgtatc cgcttcccga ttctccttca gctttcttgt ttcaagttct ttcgtatgaa
     1261 tctcattttc catctcgagc tgactgatcc tggccttctt tttctgttca tttcggaaag
     1321 gactctggag atcattccag agactggagg aactgacgac agcctcgcgg atctgaaccg
     1381 taatgatctt gatacccaga ccttcctggc cttctgcgac atcgcgaagt
//
LOCUS       DAOQGJ010001289         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70387_length_1429_cov_3.652838, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001289 DAOQGJ010000000
VERSION     DAOQGJ010001289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70387_length_1429_cov_3.652838"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PK683_17235"
     CDS             complement(<1..368)
                     /locus_tag="PK683_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNH10246.1"
                     /translation="MAKKEQKIKHSAIIRFCGDSGDGMQITGSQFTATTAAFGNDLAT
                     FPDYPSEIRAPAGTVAGVSGFQLNFASNRIHTPGDTPDVIVAMNPAALKANIQDLKTN
                     GILIVNVDAFTDKDLEKAGYS"
     gene            997..>1429
                     /locus_tag="PK683_17240"
     CDS             997..>1429
                     /locus_tag="PK683_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10247.1"
                     /translation="MKKEITDQLTRLGCRIRENKEGKTFTRDGKIIKISSEMYSISDS
                     LMSGKKFYFDRWNDGYPSMELTPSGPDEIIEYPCLEAAPYISIFADSHYSYYLFRVDD
                     KNIENPKVYFIDHDGYDEEDAYEVFPDTVSFLSALHTKKEAN"
BASE COUNT          404 a          296 c          300 g          429 t
ORIGIN      
        1 gaatatccgg ccttttccag atccttatcc gtaaatgcat ccacattgac gatgagaatt
       61 ccgtttgttt tcagatcctg tatgtttgct ttcaaggcag caggattcat tgctacgata
      121 acatcagggg tatctccagg ggtatgaatt cggttggatg cgaaattcag ttgaaatccg
      181 gagactcctg ccacagttcc tgcaggtgct ctgatttccg acggataatc aggaaaagta
      241 gccagatcat ttccaaacgc tgccgtagtt gctgtaaact gacttccggt aatctgcatt
      301 ccatcaccgg agtcaccgca gaatcttatg attgcggaat gttttatttt ctgttctttc
      361 tttgccatat ccgttaaaga gtccccttca tatgtgtatg aatttgagca tatttctttc
      421 tgacccttcg aaggcgatat ctttttcttc aatcagtcag tcttttgaaa tattccagtc
      481 atagatataa gcctcatatg cttccgggcg gtattttttt aacggttctt attttttcag
      541 gaataccgca ccgatccttt cggaatttcc ctttactagg aagtacacag tggcactgtc
      601 tacatcagaa ttgttactga gaatgaatat ctgattccca taccggaata atgtcctgct
      661 tgctccccgt attgatactg aaaatctttc ttactgagac ttccgtatgc agtgacaggg
      721 cgtgagaaat gtaattttac ctgagcgaaa acctgctgat atgagggttt gttctctgca
      781 gaaacaggaa gggttcttaa tgatcatcct agaaagaaat tatactgcgc agcgcctgct
      841 gaggcctttg ctcggtacgc atacgtgctg cacttaatac ttgaacctga gaggcgttag
      901 atttttctcc ttttcagaaa atataaagag gaaaaatgct ttcaaaagag acttttcctg
      961 aattacacag gaagcttcgg caagggagga gctcatatga aaaaagaaat caccgatcag
     1021 ctgacaagac tcggatgcag aatcagagag aacaaagagg gaaaaacatt cactcgcgac
     1081 gggaaaatca ttaaaatatc ttcagaaatg tacagtattt cggacagcct gatgagcggg
     1141 aagaaatttt actttgacag atggaatgac ggatatcctt cgatggaact gactccttcc
     1201 ggccctgatg aaatcattga gtatccctgt ctggaagctg ctccatatat cagcatattc
     1261 gctgattctc attactctta ttatcttttt cgagtagatg acaaaaacat tgaaaatccg
     1321 aaagtttatt ttatagatca tgacggttat gacgaggaag atgcctatga ggttttcccg
     1381 gacacggtaa gttttctatc agctctgcac accaaaaagg aagcgaatg
//
LOCUS       DAOQGJ010001290         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70394_length_1429_cov_3.405386, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001290 DAOQGJ010000000
VERSION     DAOQGJ010001290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70394_length_1429_cov_3.405386"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK683_17245"
     CDS             <1..370
                     /locus_tag="PK683_17245"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH10248.1"
                     /translation="AVFGAPVDIGNPSEKALKAAKEICRQLKKEISLGKVPPVKIGIG
                     IHSGNVVTGSIGSEERKEYTVIGDAVNLASRIEQLNKQYGSEILFSEDVWMDLKHKEG
                     SDLGEITVKGRTQPVKIYKL"
     gene            367..>1429
                     /locus_tag="PK683_17250"
     CDS             367..>1429
                     /locus_tag="PK683_17250"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH10249.1"
                     /translation="MIRKIHGISFKINSIFIISMLLVISAVGIPFYYMYSKYMMKSVV
                     DVLRYSVQTAEICIEKADISAMKPESFRSQEYGTVWRRLKYLQETYQLRYLYILRKDS
                     DGFRFIYDTQDDPDVKATETEKGKYRLEFYPDVKEERRARPFGQLDDDNYFTVYSDAP
                     KEVEKSYSSRTMIEADPYTDKWGTFKSVYRPLIKDGEIIAVIGADYDLSYIISMQRKA
                     VILLLSVVSAGLLVSLLMRFLIHRLIIRPILRLNQGSEELAHGNLDYKIDIDRKDEIG
                     SLGKSFNYMAENLKSSFRQIKDYNEKLEEKVAIRTSELSATLDKVQLLKNQQDGDYFL
                     TTLLMNPLMQNRSRSRFIKI"
BASE COUNT          467 a          252 c          338 g          372 t
ORIGIN      
        1 ggcggtcttc ggggctccgg ttgatatcgg aaatccatca gagaaagcac tgaaagccgc
       61 aaaggaaatc tgcagacagt taaaaaaaga aatcagtctt ggaaaagttc cgcccgtaaa
      121 aatcggaata ggaattcatt ccggcaatgt tgtcactgga agtatcggaa gtgaggaaag
      181 aaaggaatat actgtaatcg gtgacgcggt caatcttgca tccaggattg aacagctgaa
      241 caagcagtac ggttccgaaa ttcttttttc agaggatgta tggatggatc tgaaacacaa
      301 agaaggatca gatttgggtg aaattacagt aaaaggcagg actcagcctg taaaaatcta
      361 taaattatga tcagaaaaat tcatggaatc agttttaaga taaactctat ttttattata
      421 agtatgctcc tggtgatttc ggcagttggt ataccatttt actatatgta ttcaaagtat
      481 atgatgaagt ccgttgttga tgttctgaga tactccgttc agacagctga gatctgcatt
      541 gaaaaagccg atatttctgc aatgaagccg gaaagtttca gatcacagga gtatggcact
      601 gtctggcgca ggctgaaata tcttcaggaa acctatcagc tcagatatct ctatattctg
      661 cgaaaggatt ctgacggatt ccgttttata tatgacaccc aggatgatcc tgacgtaaag
      721 gcaacggaga cagaaaaagg aaaataccgt ctagaatttt atcctgatgt caaggaagaa
      781 agaagagcgc gtcctttcgg tcagctggat gatgacaact acttcacagt atattcagat
      841 gcacctaagg aagtggaaaa atcgtattca tccagaacga tgattgaggc tgatccatat
      901 acggataagt ggggaacatt caagtcggta tacagacctc tgataaaaga tggagagatc
      961 attgcagtca taggtgcaga ttatgatctg tcttatataa tcagtatgca gaggaaagcg
     1021 gttattctcc tgctttctgt tgtcagtgca ggtcttctgg tatcgctcct gatgagattt
     1081 ctgatacata gactgatcat cagacccata ctgagattaa accagggttc agaagaactg
     1141 gctcatggaa acctggacta caagatcgac atcgacagga aggatgagat tggcagtctg
     1201 gggaaatcgt tcaattatat ggcagaaaac ctgaaaagct cctttcggca gatcaaggat
     1261 tataatgaaa aacttgagga aaaggtggca atcagaacat cagaactgag tgcaactctt
     1321 gataaggtcc agcttttgaa aaatcagcag gatggagact attttctgac gacacttctg
     1381 atgaatcctc tgatgcagaa tcggagcaga agccgattta ttaagatcg
//
LOCUS       DAOQGJ010001291         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70402_length_1429_cov_3.233624, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001291 DAOQGJ010000000
VERSION     DAOQGJ010001291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70402_length_1429_cov_3.233624"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1009)
                     /locus_tag="PK683_17255"
     CDS             complement(185..1009)
                     /locus_tag="PK683_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10250.1"
                     /translation="MIKKLIVFLICILPVSAEKLAEWDGVSAEKTVDGNIFLSMQEKK
                     TGTELFKEEYFHDFTSSFYTNGSRKYFVITYFSGGESGCCNVLKFFYMKSGHLKTLSL
                     ENSVYAEPSIVDLDGDKKEEIRTDSELFYGLKLTGRKNTCTVTEPAYQGMGKIRFPEY
                     SVIEESSPESRIKEVTFHKKYYEYLKPYFKEAEKFLSENRKKTINDDDNETAGLLQYL
                     HYKQRIGEGREAEKNIENADLSIQYSCENDYGKKTEMKEKLHEFIRKNRKFLRIGK"
     gene            complement(1015..>1429)
                     /locus_tag="PK683_17260"
     CDS             complement(1015..>1429)
                     /locus_tag="PK683_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10251.1"
                     /translation="TSFLLNENPEDLLVKDYGPAVLIVPYFMRRPYDIFLVLKDTEKM
                     FLHELNDEELTAVTTAWQETVRVLLQLLHNFGKEEAYNVTVNNGPGAGLYLEFLPFTQ
                     LIGGYEKIGLWVCQESPFKAAETIREYFHNLTPRA"
BASE COUNT          386 a          310 c          264 g          469 t
ORIGIN      
        1 gtcatctgta tttttttccg gaacagtttc cgctggcagg gataaaaaac tgcctgtggc
       61 agcataaaaa ataacaatta tataatatat aaattttgtg ttaatggtca tatatattcc
      121 tcaaatctct aatagcggag cgtcgcaagt acggagatcc gttcagcacc aattttcctc
      181 gaagctattt cccgattctg agaaattttc tgttcttgcg gataaattcg tggagttttt
      241 ccttcatctc agttttcttt ccatagtcat tttcacagga atactgaatg cttagatcgg
      301 cattttctat atttttttct gcctctctgc cttcaccgat tctctgcttg taatgaagat
      361 actgcagcag tccggcagtc tcattgtcgt catcattgat tgtttttttt ctgttttcag
      421 aaagaaattt ttccgcttcc ttgaaataag gtttcagata ttcataatat tttttatgaa
      481 atgtcacttc cttgatcctg ctttcaggag aagattcttc gatgactgaa tattccggaa
      541 aacgaatctt gcccattccc tgataagcag gctcagtcac agtgcaggta ttttttctgc
      601 cagtcagttt cagcccgtaa aaaagctcac tgtctgtcct gatttcttct tttttgtccc
      661 catccagatc cacgatggag ggttcagcat aaaccgagtt ttcaagcgac agtgttttga
      721 ggtgccctga tttcatataa aaaaacttca gcacattgca gcacccgctt tctccgccgg
      781 aaaaatatgt aattacaaaa tattttcttg aaccgttagt ataaaaactg ctggtaaaat
      841 catggaagta ttcttcttta aaaagctccg ttcctgtctt tttttcctgc atagaaagaa
      901 atatattccc gtccactgtc ttctctgcgg aaactccgtc ccattcagcc agcttttccg
      961 cagataccgg aagaatgcat atcagaaaaa caataagttt ttttatcata ttctttatgc
     1021 gcggggagtc agattgtgaa agtattctct gatggtttcc gccgccttga acggactttc
     1081 ctgacacacc cagagtccga ttttttcata tcctccgata agctgggtaa agggaagaaa
     1141 ttcgagatag agacccgcgc caggcccatt gtttacagtg acattatatg cttcttcctt
     1201 gccgaaatta tgaaggagct gaagaagtac ccgtactgtc tcctgccagg cagtcgtaac
     1261 ggctgtcagt tcttcatcat tcagctcatg cagaaacatt ttttcagtat ccttaagaac
     1321 gagaaaaata tcgtaaggtc ttctcataaa atatggaaca ataagcactg ccggaccgta
     1381 gtctttgacg agaagatcct caggattttc attgagaaga aagcttgta
//
LOCUS       DAOQGJ010001292         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70440_length_1429_cov_2.292576, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001292 DAOQGJ010000000
VERSION     DAOQGJ010001292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70440_length_1429_cov_2.292576"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..>1429
                     /locus_tag="PK683_17265"
     CDS             226..>1429
                     /locus_tag="PK683_17265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNH10252.1"
                     /translation="MELDSRKQKLILGILFIAALGLRLWNVTNDLNDFHHFRQTYTAA
                     FAKHFYEYSMNIFKPNLDILNYKNISEFQIYAYMVAVFYKIFGFHDIIGRFISIFFSA
                     LSFFVFYRLVEKVYDSITALIAAFLFSFLPLMIFYSRVFMLESMMLFLSISLIYTSLL
                     WIETEEKKYMAAAGVFTALTLLIKIPTLYMLIPVAFMILNRYGFRFLLMPRFYIFGVL
                     SITPALYWYFLHARLFPEDSIVDKEAVGIYYSREAWEYYFYLMKNPATWAKIFLEHIA
                     EYHLAVSVFLFALAGIFLNFRSAFSLKKTFNYTPNSWIFPFWVLGFIVFILAFIAPNA
                     AHEYYQLPIVPPMIAIAAFYLRNILEMYLQNRSSALMKTALLIHAILLLSILPFSYGK
                     LKARLTPDP"
BASE COUNT          383 a          304 c          254 g          488 t
ORIGIN      
        1 tccttccctc tttttattta ttactttata ttttattttc cttattttca ttttccataa
       61 cgttcagcaa gggctgaaaa caaaaagaat ctgacagatc ctcaaaaaga gtcaagaatt
      121 cttttttcaa aaaaacagaa aaatttgccg gaataatttt gtatttacac tcattcaatt
      181 ggaaaaaatc taaacataga ataaatcctg cactgcgagg aaatcatgga actggattcc
      241 agaaaacaga agctgattct gggcatttta tttattgcgg ctctcggact gaggctttgg
      301 aacgtcacca atgatctgaa tgattttcat catttcagac agacttatac tgcagcattt
      361 gcaaaacatt tttatgaata ttcaatgaat atattcaagc cgaatctcga tatcctgaat
      421 tacaagaata tctctgagtt tcagatctac gcatatatgg tcgcagtatt ttacaagata
      481 ttcgggttcc atgacattat cggccgattc atatccattt tcttttctgc actgagcttt
      541 tttgtatttt acagactggt ggaaaaggtc tacgacagca ttactgcact cattgccgca
      601 tttctcttct cctttctccc tctgatgatt ttttattcaa gagtgtttat gctggaatcc
      661 atgatgcttt ttctttccat ttcgctcatc tacacatcgc ttctctggat tgagacggaa
      721 gagaaaaagt atatggccgc agccggagtg tttactgcac tgactctgct gatcaagatt
      781 cccactctgt atatgctgat tcctgtcgca ttcatgatcc tgaacagata cggattccga
      841 ttccttctca tgcccaggtt ttacatattc ggagttctga gcattactcc tgcactgtac
      901 tggtattttc ttcacgccag gctgtttcct gaggattcca ttgtggacaa ggaggcggta
      961 gggatttatt acagcagaga agcatgggaa tactattttt atctgatgaa aaatccggca
     1021 acatgggcga agattttcct tgagcatatt gcagaatatc atctggctgt ttccgttttt
     1081 ctttttgcac ttgcgggaat tttcctgaat ttccgatctg cattctcctt gaaaaaaacc
     1141 tttaattaca cgccgaattc ctggatattt cctttctggg ttctgggatt catagtattt
     1201 attcttgctt tcattgcacc gaatgcggcg catgaatact atcagcttcc tattgttccc
     1261 ccgatgatcg caatcgccgc attttatctt cggaacattc ttgagatgta tcttcagaac
     1321 cgaagcagtg cattaatgaa aacagcatta ttgattcatg caatactgct tctgtccata
     1381 cttccgttct catacggaaa actgaaagcg agactgacac ctgatcctt
//
LOCUS       DAOQGJ010001293         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70681_length_1426_cov_3.049599, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001293 DAOQGJ010000000
VERSION     DAOQGJ010001293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70681_length_1426_cov_3.049599"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PK683_17270"
     CDS             <1..348
                     /locus_tag="PK683_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10253.1"
                     /translation="KKYVNRKTLIEDPIQGLLNTKLIDDRNDIVSVWEFNADYGYPTP
                     SLERDGIIKKILPELDKIGIYSRGRFGGWKYEVSNQDHAMMQGVEWANMILDGIPEIT
                     LFHPSVANANYAK"
     gene            363..>1426
                     /locus_tag="PK683_17275"
     CDS             363..>1426
                     /locus_tag="PK683_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH10254.1"
                     /translation="MPLSGKKVLFVVTEDWFFWMHRRNLALFLKEKGAEIYVASRKGT
                     YFNSILNDGFHFTEFEISRKSLNPFLRLRNIFHLKKILNQVSPDILHCVSIQPILIGA
                     FASIGKKHLTVLAFTGLGYLFSENDLKRKTVRSLILSMLRYLFRLIHPVCIFENSDDE
                     NEIRLKTGGTADSYSTVPGSGVDLKLYSFVPEKPGGKRVLFSGRLLKEKGAELYAEAA
                     AVLKKKNPEYEFLMAGPLDSENRGAVSEKELKKWTLSGNVEWLGNVTDVAGLLKSINI
                     VCLPTYYREGIPMALIEASASGRSAVTTDTPGCREIIKDGVNGILIRPKDLGSLVSAL
                     EFLLENPEIRAEHGRKGREIA"
BASE COUNT          454 a          264 c          342 g          366 t
ORIGIN      
        1 aaaaaatatg taaacagaaa gactttgatc gaggatccaa ttcagggact tttgaataca
       61 aagctgatag atgacagaaa tgacatcgtg tctgtctggg agttcaatgc ggactacgga
      121 tatcctaccc cttctctgga aagagacggg ataattaaaa agatactgcc tgaactcgac
      181 aaaatcggaa tctattccag aggaagattc ggaggatgga aatatgaagt atccaatcag
      241 gatcatgcga tgatgcaggg agtggaatgg gcaaatatga ttctggacgg aattccggaa
      301 atcacactgt ttcatccttc agtggcaaac gcaaactacg caaaataagg caggaaaagc
      361 ttttgcccct gtccggaaaa aaagtgctgt ttgtggttac ggaggactgg tttttctgga
      421 tgcacagacg aaatcttgct ctctttctga aagaaaaggg agcagaaatc tatgtggcat
      481 caagaaaagg aacatatttt aattcaattc tgaacgacgg ttttcatttt accgaatttg
      541 aaatatcaag aaaaagcctg aatccttttc tcagacttcg caatattttt catctgaaaa
      601 aaatactgaa tcaggtttct cctgacattc ttcactgtgt ttccattcag ccgattctca
      661 tcggtgcttt tgcttccatt ggaaaaaaac atctgactgt tctggcgttt accgggctgg
      721 gttatctttt ttccgaaaat gatctgaaga gaaaaacggt aagatctttg attttaagta
      781 tgctgagata tcttttcaga ctgattcatc cagtgtgcat atttgaaaac tctgacgatg
      841 aaaatgaaat cagattgaaa acaggaggaa ctgctgactc ttacagcact gttcctgggt
      901 caggagtcga cctgaagctt tacagttttg tcccggagaa gcctggaggg aaaagagttc
      961 tgttttcagg aagacttctg aaggaaaaag gagcagagct gtatgctgaa gcggcggcag
     1021 ttctgaagaa aaaaaatcct gaatatgaat ttctgatggc aggtcctctc gacagcgaaa
     1081 acagaggggc tgtcagcgaa aaagaactca aaaagtggac attgtcagga aacgttgaat
     1141 ggctcggcaa tgtaaccgac gtcgcaggat tgctgaagtc aatcaatata gtatgccttc
     1201 ctacttatta cagagaaggc attcctatgg cactgataga agcgagcgca tcgggaagat
     1261 ctgcagtcac taccgataca ccgggatgca gagaaatcat aaaagacggt gtcaacggaa
     1321 ttttaatcag gccgaaggac ttaggttctc ttgtatcggc actggaattt cttctggaaa
     1381 acccggagat acgcgcggag cacggaagaa agggaagaga gattgc
//
LOCUS       DAOQGJ010001294         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70682_length_1426_cov_2.997812, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001294 DAOQGJ010000000
VERSION     DAOQGJ010001294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70682_length_1426_cov_2.997812"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK683_17280"
     CDS             complement(<1..415)
                     /locus_tag="PK683_17280"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF014870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNH10255.1"
                     /translation="MTAVSSEKGIVLLEFESIQNLQKELSEIEKILNSKITYESDSCL
                     KILEKELSEYFSGERKTFTVPLDLQGTDFQISVWKALLEIPYGSTRTYQQQSEFLGNT
                     KAIRAVGTANGKNRIAVLVPCHRVIGRDGSLVGYKG"
     gene            complement(454..1131)
                     /locus_tag="PK683_17285"
     CDS             complement(454..1131)
                     /locus_tag="PK683_17285"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HNH10256.1"
                     /translation="MIFTTAVFLFLGGSVSAGDQKVHSCSKISDEKKQASCWKAEYEK
                     ADKELNKVYKSLQTSLASAKAEEMKKDSRVWIDHKEALCSGQSGMSNDSKAVYYECLQ
                     SITSNRTRFLKEAFGGEGTDNKIEGSYSDSLGGSLTIQKASKNYKFTLEVVRGVTTHT
                     GEVSGEIALKNNTAMYDEKEKDGNEPCKISFKFSDRKVTVDEIECSSYHGASAYFGGT
                     YYKVRKR"
     gene            1147..>1426
                     /locus_tag="PK683_17290"
     CDS             1147..>1426
                     /locus_tag="PK683_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10257.1"
                     /translation="MTDINRPVFYSNCFYEKFDAEKKNPKNEKMEKIDESGYRIIDAW
                     TQPALTSMYEKLPEVAYLLKRSGTRAHTKKSIGPDEMIAVMDEAGIEKV"
BASE COUNT          379 a          320 c          281 g          446 t
ORIGIN      
        1 ctcccttgta tccgacaagg cttccgtctc tgccgattac acggtgacag ggaacaagaa
       61 cggcaatcct gttttttccg tttgcagttc ccactgcacg gattgctttt gtgtttccga
      121 gaaattcaga ctgctgctgg taggttcttg tgctcccgta aggaatttcc agaagggctt
      181 tccagacaga tatctgaaaa tcagttccct gcagatccag aggcacagtg aatgtctttc
      241 tctctcctga aaaatattcc gagagctcct tttccagaat tttcaggcag gaatccgatt
      301 cataagtaat tttactgttt aagatttttt caatttcgga aagttccttc tgaagattct
      361 gaatgctttc gaattccaga aggacaattc ctttttctga ggaaacagct gtcattgttc
      421 caaactcaga ttggattttt cggcaccgca gattcagcgt tttcttacct tgtagtatgt
      481 tccgccgaaa taagcacttg ctccatgata ggaagaacat tcaatttcgt ccacggtaac
      541 ttttctgtcg gaaaatttga aactgatctt gcaaggttcg tttccgtctt tttccttctc
      601 gtcgtacatt gctgtattgt ttttcagtgc aatttctcct gaaacctcac cggtatgagt
      661 tgtgacacct ctgaccactt ccagtgtgaa cttgtagttt ttagatgcct tctgaattgt
      721 aagagatcct ccgagagaat cagaataact tccttctatt ttattgtctg tgccttcccc
      781 tccgaaagcc tctttcagaa acctagttct gttagacgtg atagattgca gacattcata
      841 ataaactgcc ttagaatcat tcgacatacc gctttgacct gagcagagcg cttccttgtg
      901 atcaatccat acgcgggagt cttttttcat ctcttctgcc tttgctgatg ctagacttgt
      961 ctgaaggctt ttataaactt tgttgagttc cttatctgcc ttttcatatt cggccttcca
     1021 gcaggatgcc tgttttttct catcactgat cttggaacag gaatgaactt tctgatcacc
     1081 cgcagaaacg gaaccgccga gaaaaaggaa tactgccgtt gtgaaaatca attttatcat
     1141 cataagatga ctgatataaa ccgccctgtt ttttactcaa actgttttta tgaaaagttt
     1201 gacgcggaaa agaaaaaccc caaaaatgag aaaatggaaa aaattgacga atccggatac
     1261 agaatcattg atgcctggac acagcctgct ctgacatcga tgtatgaaaa gctccctgag
     1321 gtcgcttatc ttctgaaacg ctccggcaca agagcacata caaaaaaaag catcggaccc
     1381 gatgaaatga ttgcagttat ggatgaggca ggaatagaaa aggttc
//
LOCUS       DAOQGJ010001295         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70698_length_1426_cov_2.644055, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001295 DAOQGJ010000000
VERSION     DAOQGJ010001295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70698_length_1426_cov_2.644055"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK683_17295"
     CDS             <1..262
                     /locus_tag="PK683_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004768921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH10258.1"
                     /translation="DVLVLDSQYTLDESFAKFDWGHTSYTMAVNCATIWGIKTLVLTH
                     HEPAYNDRKVFEILEEAVEHKRNLGGNPLELILAREGLILEL"
     gene            259..>1426
                     /locus_tag="PK683_17300"
     CDS             259..>1426
                     /locus_tag="PK683_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNH10259.1"
                     /translation="MKKRGLYFAGIILFVLASLSGGFFGYILSEVNKGEELYLLTTYQ
                     PTTPTKLYDVNGVPFAELYRHRQELIRYRDIPPHVVHAFISVEDDNFFNHFGIDFIAI
                     IRAAWVNMTSLKIKQGGSTITQQLSKAILKNTRKSFTRKFIEALLTLQIEQEYSKEEI
                     LEIYFNLVYLGHGTTGLASAANVYFSKDVRDLDIAEAALLARLPKAPVQYSPFKHSLT
                     AKKAHKTVLGLMARNGFIPKDQIPKIHDEFWEKYWPIVITKSPSSSTWGTKLDRAPYF
                     TEHIRKQLIKELGEELVYTGGLKVYTTLDIRKQEIAEEELLKALVSYDKTSFGASLSY
                     KGGVDTSLVGLYSMLGSIFPIAGLDVSRFDEATSFRVSMESELIEAMDILTILSP"
BASE COUNT          397 a          310 c          336 g          383 t
ORIGIN      
        1 agacgttctg gttctggatt ctcagtatac tctggacgaa tcatttgcta aatttgactg
       61 gggtcataca tcctatacaa tggccgtgaa ctgtgccaca atctggggca tcaagactct
      121 tgtgctgact catcatgaac ctgcttacaa tgacagaaag gtatttgaga ttctggaaga
      181 agcagtggaa cacaaaagaa atctgggcgg aaatccgctg gagctgattc tggctcggga
      241 aggactgatt ctggaattat gaaaaaacgc ggtctttatt ttgcagggat cattcttttt
      301 gtccttgcgt cactttctgg cggttttttc ggatatattc tatctgaagt aaacaaagga
      361 gaggaactct atcttctgac aacttaccag cctacgacgc ctacaaaact gtatgacgtc
      421 aacggtgtac cattcgccga gctgtacaga cacaggcagg aactgatccg gtacagggac
      481 atccctcctc atgtggttca tgcctttatt tcagtggaag acgacaattt ttttaatcat
      541 tttgggatag attttattgc gatcatccgg gcggcctggg tcaatatgac cagtctgaaa
      601 atcaagcagg ggggatctac aatcacccag cagctctcca aggcaattct gaaaaatacc
      661 aggaaaagct ttacaaggaa attcattgaa gctcttctga cccttcagat agaacaggaa
      721 tattccaagg aagaaattct ggagatatat ttcaatctgg tctatcttgg tcatggaacg
      781 acaggacttg cttccgctgc gaatgtttat ttttcaaagg atgtccgaga tcttgatatt
      841 gctgaggctg cacttctggc acgtcttcca aaggcccctg tacagtattc tccatttaag
      901 cattccctca cggcgaaaaa ggcacataag acagttcttg gactgatggc caggaacggc
      961 tttatcccca aggatcagat ccccaaaata cacgatgagt tctgggaaaa atactggccg
     1021 atcgtaatta ccaagtcacc ttccagttcc acatggggta ctaagctcga cagggctccg
     1081 tactttacgg agcatatccg caagcagctc atcaaggagc tgggtgaaga actggtctat
     1141 accggcggtc tgaaagtcta tacaactctt gatatcagaa agcaggaaat tgcagaagaa
     1201 gaacttctga aagcgttagt ttcatatgac aagacttctt ttggagcaag tctttcctat
     1261 aagggcggtg tcgataccag tctggtaggt ctgtattcga tgctggggag cattttcccg
     1321 attgcaggac tggatgtcag ccgctttgac gaagccacaa gcttcagagt atcgatggaa
     1381 agtgagctga ttgaagcaat ggatatcctg accattctga gtccag
//
LOCUS       DAOQGJ010001296         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70780_length_1425_cov_2.777372, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001296 DAOQGJ010000000
VERSION     DAOQGJ010001296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70780_length_1425_cov_2.777372"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PK683_17305"
     CDS             complement(<1..822)
                     /locus_tag="PK683_17305"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNH10260.1"
                     /translation="MKYRELKAAVPKTIADDFFLFLDDLKTEGYYEILFDSSIPKKPE
                     EGIIRDDTFVNIYLSETDLERELKVMIWLKAAAPESFFMESRTVETREFEEAYKEFYK
                     PFVIGDRFILIPTWEKESEEAKKLLETKDRIVMYMNPGMAFGTGHHETTKLMLAMIGK
                     LIQKDMKILDMGCGSGILSIAAALSGASEITAIDVDPNAVRATEHNWGENSYPNLKEV
                     RILEGGFDHPAAWDRDYDFLLANITYGVISQNMERIKKIRTDRYLFSGIITERKQD"
     gene            complement(819..>1425)
                     /locus_tag="PK683_17310"
     CDS             complement(819..>1425)
                     /locus_tag="PK683_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10261.1"
                     /translation="FFAGSDGKDDLLSRDADGTPEICLDISSFLRTIENTEGIMKNIH
                     EFYSDPEKLTDDVSLIRIEIRSLVSKKSRNLKEVFPSSGRQNLNSAVTFMKTLYKAMP
                     ANETALRFLSLYYFRRREFNYALRLANAYNNQCPDSWNMLYLESVLWKKMGNFRRALS
                     ISEQIYLRNPDIIKNLKNRKELFSLVQKIDSSRQKLVSGIK"
BASE COUNT          337 a          320 c          286 g          482 t
ORIGIN      
        1 gtcctgtttt ctttcagtga tgattccgct gaagagatac ctgtcagtcc ggatcttttt
       61 gatcctttcc atattctgag agatgactcc gtatgtgatg tttgccagaa gaaaatcata
      121 gtccctgtcc caggccgccg gatgatcgaa tcctccttcc agtattctga cttctttgag
      181 attcggataa gagttctctc cccagttgtg ctcagtcgcc ctgactgcat tcggatcgac
      241 gtcgatcgca gtgatttcac ttgcaccgga gagtgcagcc gcaatcgaaa gtattcctga
      301 accgcatccc atatccagga tcttcatatc cttctgtatc agttttccga tcattgcgag
      361 catcagtttc gttgtttcgt gatgtcctgt cccgaatgcc attccgggat tcatgtacat
      421 tacgatcctg tcctttgttt ccagaagttt ttttgcttcc tctgattctt tttcccaggt
      481 gggaatcaga atgaatctgt cgcctatcac gaagggttta taaaattctt tataagcttc
      541 ttcaaactcc cttgtttcca cagttctgct ttccatgaag aaactttcgg gtgcagccgc
      601 cttgagccag atcatcacct tgagctctct ttcgagatca gtttcactca ggtatatgtt
      661 tacgaaagta tcgtctctga tgatgccttc ttccggtttt ttgggaatgc tggaatcaaa
      721 cagaatttca tagtatcctt ctgtcttcag atcatcgagg aagaggaaaa aatcatctgc
      781 tattgtcttt ggtacggctg ctttcagttc tctgtatttc attttattcc cgatacaagt
      841 ttttgtctgg aagaatctat cttctgtacc aatgaaaaaa gctcctttcg gtttttcaag
      901 tttttaataa tatcaggatt cctgagataa atctgttccg atatgcttag ggctcttctg
      961 aaattgccca tttttttcca gagaaccgat tccagataca gcatattcca ggaatcggga
     1021 cactgattgt tgtatgcatt tgcaagacgc agagcatagt tgaattctct tcttctgaaa
     1081 tagtacagtg aaagaaaacg cagagctgtt tcatttgcag gcattgcctt gtacagcgtc
     1141 ttcatgaagg tgactgctga attcagattc tgtcttcctg agctggggaa cacttctttc
     1201 agatttctgc ttttctttga aaccagggaa cggatttcaa ttcggatgag cgaaacatcg
     1261 tctgtcagct tctcaggatc ggaataaaat tcatgtatat ttttcattat gccttcagta
     1321 ttttctattg ttctgagaaa actgctgatg tccaggcaga tttcaggggt tccgtctgca
     1381 tctcttgaga gaagatcatc ctttccgtcc gaaccagcaa aaaaa
//
LOCUS       DAOQGJ010001297         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70796_length_1425_cov_2.421898, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001297 DAOQGJ010000000
VERSION     DAOQGJ010001297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70796_length_1425_cov_2.421898"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK683_17315"
     CDS             <1..248
                     /locus_tag="PK683_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10262.1"
                     /translation="LIDHINRSLNTLPEKTEEELRHLESVSFFRGKRLSMKKDGKTVF
                     PVFKGFSPEGFLVLDLEGKETVISDSAREIECNAACN"
     gene            232..1002
                     /locus_tag="PK683_17320"
     CDS             232..1002
                     /locus_tag="PK683_17320"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNH10263.1"
                     /translation="MLLVIDVGNTNTVFGIFAKGENEPKFHKRTVTRPDRTSDELGVF
                     LKGFFREENLESVPISSAVYSSVVPAFNPIVSRMLEDWFKVKPLKVSCEMNLPFIIKY
                     PRPYEIGADRLVNAAAVAAESSENSIIVDMGTATTFCVLNSKKEYLGGVIAPGLKLSM
                     EALTKNTAQLPPIVFQTPERILGNSTIESLQSGFFYGWVGLLEGIIREIKGQYGEDYK
                     VIGTGGLVSAIHSAKNNIFDKVDPLITLRGLKILNDMN"
     gene            1074..>1425
                     /locus_tag="PK683_17325"
     CDS             1074..>1425
                     /locus_tag="PK683_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10264.1"
                     /translation="MRPLLEDLKVNLELNKNPSDTVLQALNFYCSLKDNDSYEKRIKD
                     ELLLILSEKRTSKAYFNIPFQNSRLKPSGYNQIIMVLLKRIWEEEDFTRMTKLPKEIR
                     FLNLDETGYSGARIS"
BASE COUNT          446 a          281 c          322 g          376 t
ORIGIN      
        1 gtctgattga tcatatcaac agatcactga atactctccc tgaaaaaaca gaagaggaac
       61 tgcgtcatct ggaatctgtt tccttttttc gcggaaagcg gctcagtatg aagaaggacg
      121 ggaagacagt ttttcctgtg ttcaagggtt tcagcccgga gggttttctt gttctggatc
      181 tggaaggaaa agaaactgta atcagtgatt ctgcaaggga aatagaatgc aatgctgctt
      241 gtaattgatg ttggaaacac aaacactgta ttcggtattt ttgcgaaagg cgaaaatgaa
      301 ccgaaatttc acaaaagaac cgtcacaagg cctgacagaa cttcagacga acttggagtc
      361 ttcctgaagg gatttttcag agaggagaat ctggaaagtg tgcccatttc ctctgctgtc
      421 tattccagcg tcgtgccggc tttcaatcct atagtctcca gaatgcttga ggactggttc
      481 aaggtcaagc ctctgaaagt cagctgtgaa atgaatcttc ctttcattat aaaatatccc
      541 aggccgtacg agatcggcgc agacagactt gtcaatgctg ctgcagtcgc tgcagagtca
      601 tcggaaaaca gcatcattgt ggatatggga actgccacaa ctttctgtgt gctcaattcc
      661 aaaaaggaat atttgggagg agtcatagca cccggactca agctttccat ggaagcattg
      721 acaaaaaata cggcacagct gcctccgata gtatttcaga cacccgaaag aattttggga
      781 aattccacga ttgagtctct tcagtccggt tttttttacg gctgggtggg actcctcgaa
      841 ggcatcatcc gcgaaatcaa gggtcagtac ggggaagatt ataaagtcat cggaaccggg
      901 gggcttgtat ctgcaattca ctcggcaaaa aataatatct ttgacaaagt agatccgctg
      961 ataactctaa gaggattgaa aatactgaat gatatgaact gattttttcg tcttatatca
     1021 cagattctta tatcgcagat tcaattatca atgtaaatgg aacaggaaag aaaatgagac
     1081 cgcttctcga agatctaaaa gtaaatctcg aactgaataa aaatccaagt gatactgtac
     1141 ttcaggccct caatttctac tgttcgttga aagacaatga cagctatgaa aagagaataa
     1201 aagacgaact tttgcttatc ctttctgaaa aaagaacaag caaggcctat tttaatatcc
     1261 cgtttcagaa cagcagactg aaaccctccg gatacaatca gatcattatg gtccttctga
     1321 aaagaatctg ggaagaagag gattttacca ggatgacaaa acttccgaag gagatccggt
     1381 ttctgaatct ggatgagacg ggatattcag gtgcaaggat ttcag
//
LOCUS       DAOQGJ010001298         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70813_length_1425_cov_2.196350, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001298 DAOQGJ010000000
VERSION     DAOQGJ010001298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70813_length_1425_cov_2.196350"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PK683_17330"
     CDS             complement(<1..1004)
                     /locus_tag="PK683_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH10265.1"
                     /translation="MDYAGILLSVRSRYLSTVEAVTYFLAVPFGLVFLFFSASYELKE
                     IRAISFGAAAACIMTIIIFYPIRRNKIDSVLGGNSVFQEKEHLKKMNEMLHSIPRFES
                     FLISIRWLAGITAALFFSHLAEPAGGVRILSFAVLYFLMFPVNWISAYLAAENSVNPL
                     FMHPAFSEISSVSEYKIMTFRSKIFCCFSAVVLQLAVPFSFLMYMFWRDLIKFQHLEM
                     TLGLFLFFAILLSYFVSRMLLENFSFSVDMIREISEEFSSGNFQLKANPLISRELSQP
                     LNSLREKMAAISERIKSESEELLKCANRMNLLAEDKKQEISRQSSSIGEISAAMDELN
                     "
     gene            complement(1076..1369)
                     /locus_tag="PK683_17335"
     CDS             complement(1076..1369)
                     /locus_tag="PK683_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10266.1"
                     /translation="MYKQVLGRALNEILFDPRNLGSFRKEFKEWCDTDWFPRALSYGL
                     KRIANLLPTSELAKMSLDQILTKVDNFESQYFDDFDKAAEWLKRPYLGRAVNQ"
BASE COUNT          442 a          304 c          289 g          390 t
ORIGIN      
        1 ctgttcagtt catccatcgc tgcagaaatt tctccgatag aactgctttg acgggaaatt
       61 tcctgtttct tgtcttctgc aagcagattc atcctgttcg cacatttcag aagttcttcc
      121 gactctgact tgatcctttc tgaaatggct gccatctttt cacgcagaga attcaacggc
      181 tgactgagtt ccctgctgat gagcggattt gccttcagct gaaaattgcc gctcgaaaat
      241 tcttcagaga tctcacggat catatcgact gaaaagctga aattttcaag aagcatcctg
      301 gatacaaaat aggacagcag tattgcaaaa aaaagaaaaa gtcccagggt catttccaga
      361 tgctgaaatt ttatcagatc cctccagaac atgtacatca gaaaggaaaa aggaacagca
      421 agctgcagaa caactgcaga gaaacagcag aatatttttg atctgaatgt cattatttta
      481 tattcagaaa cagaactgat ctcagaaaaa gccggatgca taaacagagg gttcactgaa
      541 ttttcagcgg cgagatatgc agatatccag ttgactggaa acataagaaa gtaaagcact
      601 gcaaaactca gaatcctgac ccctcctgca ggttcagcaa gatgggaaaa gaaaagggca
      661 gcagttatcc ctgcaagcca tctgatactg ataagaaaag attcgaatct gggaatggaa
      721 tgaagcattt cattcatttt tttcagatgt tctttttcct ggaaaacgga atttccgccg
      781 agaacagaat ctatcttatt tctgcgaatg ggataaaata taatgattgt catgatgcat
      841 gccgctgctg ccccgaatga aatcgccctg atttccttca gttcatagga ggccgagaag
      901 aaaaggaata ctaaaccgaa aggaactgcc agaaaataag tcactgcctc taccgtggaa
      961 aggtatctgc tccttacaga aaggagaatg cctgcgtaat ccatatctac tccgtctcag
     1021 aattttttca gaagaaaatg aggaaaccgc aagtctatcc cggcttcctg atgtgttact
     1081 gatttactgc tctgcctaaa taaggcctct tcagccattc agcagccttg tcaaaatcgt
     1141 cgaaatactg ggattcaaaa ttatccactt tcgtaagaat ctgatcgagt gacatcttgg
     1201 caagctcgct tgtcggaaga agattggcaa ttctcttcag tccataactg agtgctctcg
     1261 gaaaccagtc agtatcgcac cattccttga actcttttcg gaaacttccc aggtttctgg
     1321 gatcaaaaag aatttcattt agagcgcgtc ccaaaacctg cttgtacatt ttttagtata
     1381 attagtattg gttatatgag tcaagataga tttgatgtgc cagat
//
LOCUS       DAOQGJ010001299         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70939_length_1423_cov_3.342105, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001299 DAOQGJ010000000
VERSION     DAOQGJ010001299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70939_length_1423_cov_3.342105"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PK683_17340"
     CDS             <1..1182
                     /locus_tag="PK683_17340"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNH10267.1"
                     /translation="SDISGRIRQIFGDNTADHLMEAALEKRGISAFGFISDSEFYRSN
                     RFGQYFFINGRPVELRQSSYLLKKAYDELLPPGAHPWCFIYFSIDPAFIDVNVHPAKK
                     EIRFLDEEGFTGFFLELVRNVLYSKTPVGFLEMKRRMSSPIVPYSSQPSANAVQQKES
                     FFQSILHENIFPEVSRQEGFATTDVGSGVRLETLSETGRSSRDFILKKHYGVIFGTFI
                     LAEAEDGLYIIDQHTAHERIRYEEILNSLKNKTYSSQILLSPIRIDLSIEEAESVLSL
                     QKEFAQIGLSIEEFGEGTVIVRELPSYIEPGTEREVLLDFLQKFQENSFEVRNVYDLM
                     AKCSACRTAVKKGDQVSDTILAELLNRLSYCENPSRCPHGRPTMIKLTRDDLEKMFHR
                     K"
     gene            1243..>1423
                     /locus_tag="PK683_17345"
     CDS             1243..>1423
                     /locus_tag="PK683_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNH10268.1"
                     /translation="MESDLKKLLWKTLLGIVILVVLIGILSHFLKGPITQFSGWFIEY
                     LGVYGVGLGILISDAL"
BASE COUNT          399 a          295 c          332 g          397 t
ORIGIN      
        1 tcagatattt ccggaagaat ccggcagatt ttcggggaca acacggcgga tcacctgatg
       61 gaagcagctc tggaaaaaag aggaatatcg gcattcggat tcatcagtga ttcagagttt
      121 tacagatcaa acagattcgg tcagtatttt ttcatcaacg gcagaccggt tgaactcagg
      181 cagagttcct atcttctgaa gaaagcctat gatgaacttc ttcctccggg tgcacatcct
      241 tggtgtttta tttatttcag cattgatcct gcatttatcg atgtaaatgt tcatcctgcc
      301 aagaaggaaa tacggtttct ggatgaggaa ggctttacag gcttcttcct ggaacttgtc
      361 aggaatgtgc tctattcaaa aactcctgtg ggttttctgg aaatgaaaag acgaatgtct
      421 tcaccgattg tgccatacag ctcacagccc tcggcaaatg cagttcagca gaaagaatca
      481 tttttccaga gcatacttca tgagaatatt tttcccgaag tttcccggca ggaaggattt
      541 gcaacgactg acgtcggatc gggtgtacgg ctggaaactc tttcagagac gggaaggtca
      601 tcaagagact ttatactgaa aaagcattat ggagtcattt tcggaacatt cattcttgcg
      661 gaggcagagg atggactgta tatcatcgat cagcacactg cgcatgaaag aatacgctat
      721 gaagagatac tcaattctct gaaaaacaaa acctacagct cacagattct gctttcaccg
      781 attcgaattg atctgagcat agaggaggca gagtctgtac tgtctcttca gaaagagttt
      841 gcacagatcg gtctttcgat tgaagagttc ggggaaggaa cagtgattgt cagagaactt
      901 ccttcttaca ttgaacctgg aacggagaga gaggtccttc ttgactttct ccagaagttt
      961 caggaaaatt cctttgaggt ccgaaatgta tacgatctga tggcaaaatg ttccgcctgc
     1021 agaactgcag tgaaaaaagg cgatcaggtt tcggacacaa ttctggctga gcttctgaac
     1081 agactcagct actgcgaaaa tccttccaga tgtcctcatg gaagaccgac catgatcaaa
     1141 ctgaccagag atgatctgga aaaaatgttt caccgaaaat agtctgcttg acttgaacag
     1201 cacattcttt cgatgctgga ttatctttta ctgggcacgg ttatggaatc agatctgaag
     1261 aaacttctct ggaaaacgct cttaggcatt gtcatattgg ttgtactgat tggaattctt
     1321 tctcattttc tgaaagggcc gatcactcag ttttccggct ggttcataga gtaccttgga
     1381 gtctacggcg taggactcgg aattctgatc agcgatgctc ttc
//
LOCUS       DAOQGJ010001300         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_70945_length_1423_cov_3.132310, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001300 DAOQGJ010000000
VERSION     DAOQGJ010001300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70945_length_1423_cov_3.132310"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="PK683_17350"
     CDS             complement(<1..1355)
                     /locus_tag="PK683_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10269.1"
                     /translation="MKLYLEAKEASDSDALEETVPVHPAGNPFKTALSGDFDENKTAE
                     LKDKRLAELNVRSAEIHLHPSNAEKLISAFKVLRKYEYSCTEQTLSKHLPGWKLKKTF
                     DAKNASDRFDVENTEKSAQEAIDIVLNSQNTDGTWGFWYGDEGNEMLTGYVLESIYNA
                     YPEGVPADIIARLQTSFTALDRMLTNPQSTDKNIKSYIIYIASLYDRFNLPVFEQEMN
                     QQNQNAYQKANMISALKRWKVFLQKNPDLEGKSPADFAMSRIDEKMKTLSEKIKTQKK
                     SDAKGVYFEIPNQKSWQWLGSRTEVSARVLSSLLDAGEKSSLTAELYQSLLKRQSTES
                     WMSTKETASVINAAADYLRTKGIPKPAKGKVQFKFSTNDPSVVFDLDSGEKQTLVQNI
                     KLKKKTSSFKVKAEGEANSEMSYNVFLKGDLEIDEKYRPETKGLEVERKFYSVKRVKD
                     I"
BASE COUNT          328 a          315 c          287 g          493 t
ORIGIN      
        1 ttgatgtctt tgaccctctt gacggaatag aattttcttt ccacttccag tccctttgtt
       61 tccggtctgt atttttcatc aatttccaga tcacctttca ggaatacatt atagctcatt
      121 tctgaattgg cctctccttc ggctttgacc ttgaaggaag aagttttttt cttcagcttg
      181 atgttctgaa ccagggtctg tttttctccg ctgtccagat caaacacaac agaaggatca
      241 ttcgtgctga acttgaactg aacttttcct ttcgcaggtt ttggaattcc ttttgttctg
      301 agatagtctg cagctgcatt gattacggaa gctgtttctt ttgtagacat ccagctttca
      361 gtggactgac gtttcagaag ggactgatag agttctgcag tcagagacga tttttcccct
      421 gcatccagaa gtgaagaaag aacacgggcc gaaacttccg ttctgcttcc gagccactgc
      481 caggatttct gatttgggat ttcaaaatag actccctttg catccgattt tttctgcgtt
      541 ttgattttct ccgaaagagt cttcattttt tcgtcaattc tgctcattgc aaaatctgca
      601 ggagattttc cttccaaatc cggatttttc tgaaggaaaa ctttccatct tttcagagcg
      661 gaaatcatat ttgccttctg gtatgcattc tggttctgct gattcatttc ctgttcaaac
      721 actggaagat tgaatctgtc ataaagactg gcgatataga taatataact ctttatgttc
      781 ttgtccgtgg actgaggatt ggtcagcatc ctgtcgagag cagtaaaaga tgtctgcagc
      841 cttgcgatga tatccgccgg aacaccttcc ggataggcat tgtaaatgct ttccagaaca
      901 tatccggtga gcatttcatt tccttcatca ccgtaccaga atccccaggt tccgtctgta
      961 ttctgcgaat tcagaacaat gtcaattgct tcctgagctg atttctcggt gttttccaca
     1021 tcaaaccggt cagatgcatt ttttgcatca aacgtcttct tgagcttcca tcccggcaga
     1081 tgtttgctca gtgtctgttc agtgcaggaa tattcgtatt tgcgaagaac cttgaatgca
     1141 gagatgagtt tttctgcatt ggaaggatgc agatgaattt ccgcacttct tacattcaat
     1201 tccgcaagcc tcttatcctt gagctctgca gttttattct catcaaagtc tcctgagaga
     1261 gctgtcttga aaggatttcc agcaggatga acaggcactg tttcttccaa agcatctgaa
     1321 tcggatgctt cttttgcctc tagatacagt ttcatttttt ctgaatcttt agggatattg
     1381 atgctgtagt atttgtttcc tgttccgttt ggaggaagac tca
//
LOCUS       DAOQGJ010001301         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71037_length_1422_cov_3.079737, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001301 DAOQGJ010000000
VERSION     DAOQGJ010001301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71037_length_1422_cov_3.079737"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK683_17355"
     CDS             complement(<1..440)
                     /locus_tag="PK683_17355"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNH10270.1"
                     /translation="MFGNLKSLEKKLENWRSAGLISSEQSSAILDYEEKQPGKNLAVY
                     SIITLGVSVLCLGIIALIASNWESIPNFIKLSFDFIILGCTGAGVLYAEKNESQNLKQ
                     WLILALLILILASIGLISQIYNTGGEFYMAAGFWVILTLPLVIYA"
     gene            736..>1422
                     /locus_tag="PK683_17360"
     CDS             736..>1422
                     /locus_tag="PK683_17360"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNH10271.1"
                     /translation="MLETAAVSLAKEEEKPNPSVNIFPVQEETKEAIRLEEEIPNEDS
                     AKSEELNPTAILKSKDVEKPFFEVKKIQVPIPPPKEAPPAGLEETIRMPSLWDEFLYY
                     IPPFLKNRFLMLTAMTLLSLSLLIILPLKAGYDEAKLELDSLLKELGKNKPSRILDRE
                     GNKVSEIYQKKTGSMKLTEYPELLRKIILTVEDRSFYRHSGIDLASIVRAGYKNLIHL
                     RYIQGASTITQ"
BASE COUNT          440 a          312 c          295 g          375 t
ORIGIN      
        1 gcgtagatga caagaggaag ggtcagtatc acccagaacc ctgcagccat ataaaattcc
       61 ccgccggtat tgtaaatctg agagatcagc ccaatcgatg ccagaatcag tatcagaagg
      121 gctagaatga gccattgttt cagattctga ctttcattct tttccgcgta aagtacaccg
      181 gctcccgtgc agcccaatat gataaaatcg aaactcaatt tgatgaaatt cggaattgat
      241 tcccagttgg atgcgatcag tgcaatgatg ccgagacaca gtacggaaac tccaagagta
      301 ataatcgaat agaccgccag attttttccc ggctgctttt cttcataatc caggattgca
      361 gaagactgtt ccgaagaaat cagtccggca ctgcgccaat tttccagttt tttttccaga
      421 cttttcaagt tgccaaacat ttttatcccg taaggtttta agatgctgtt agtattctga
      481 gacttcaatt tcagtcaagt cttatttgct cacgggaaag agaattgaat cttgcatttt
      541 tttatttata ccgataatca gaatatggtc gggaatgcag attatttatc tttgggcgga
      601 aattttacat gcccgaaatg ccaaaaaaaa tccaggttat ccaaacttcc cgaagagcca
      661 ggtctgtaca aaatcacctg tttttactgt aagcatcaga ttctccataa aatagaggcc
      721 ccggaagaag agccagtgct tgaaactgct gccgtctccc tggccaagga agaagaaaaa
      781 cctaatcctt ctgtaaatat ttttccagtt caggaagaga caaaagaagc aattcgactc
      841 gaagaagaga tccccaatga ggactctgca aaatctgagg aactgaatcc gactgcaata
      901 cttaagtcaa aggatgtgga aaaacccttt tttgaagtta agaaaattca ggttcctatc
      961 cctcctccaa aagaggctcc gcctgcagga ttggaagaaa caatacggat gccttctctt
     1021 tgggatgaat ttctttatta tatacctccc tttctgaaaa acagatttct gatgctcaca
     1081 gcaatgactc tgctttcctt aagtcttctg atcatacttc cgctgaaggc tggatatgat
     1141 gaggcgaaac tggaactgga cagccttctg aaggaactgg gcaagaacaa accatcccgt
     1201 attctggaca gggaagggaa caaggtctcc gaaatttatc agaaaaaaac cggctcgatg
     1261 aagctgacag aataccccga actcctgcgt aaaatcattc tgacggtcga ggacagaagt
     1321 ttttacagac acagcggaat tgatctggca tccattgtaa gagcaggata caagaacctg
     1381 attcatctgc gttatattca gggggcatcc acaattactc ag
//
LOCUS       DAOQGJ010001302         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71217_length_1420_cov_3.761172, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001302 DAOQGJ010000000
VERSION     DAOQGJ010001302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71217_length_1420_cov_3.761172"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1420)
                     /locus_tag="PK683_17365"
     CDS             complement(<1..>1420)
                     /locus_tag="PK683_17365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH10272.1"
                     /translation="DAAKAKNAVKESEAKLASVLNEVQDVIWSVSLPDYKSLFVSPSV
                     EKLHGYSVKEWMDDSTLWMKVIHPDDRNIAQTINQNLEKTGNSYVEYRIICGDGSVKW
                     ISNKARIVLDENRHPFRLDGTLADITERKIAEQELLQAKLAAEEASRAKSEFLANMSH
                     EIRTPLNAIIGFSSLLKETELTAQQNSFIHNFETASAHLLNLINDILDFERLEAGMMP
                     KEPLYFNYEQFVQDIAGIYVAKAHNKQIELNFHFKPNSHLVIQSEPSFLKQILSNLVS
                     NAVKFTAVGQVLITADLFSREGRDWISLSVRDTGIGLPEESGHLFDPFVQADMSVTRK
                     YGGTGLGLAIVKKMVDSLGGTITARNMPNGAEFLAEIPAEVREESRSEIYDTELLKDS
                     RMLIFSQREGFRSIIYDYTVQWGLQSETLSEPERILPSLISAREAGNPYKIIIIDIDY
                     IESKIKDLLVQINPNQETGILLF"
BASE COUNT          373 a          332 c          273 g          442 t
ORIGIN      
        1 aaacaaaagt atccctgttt cctgattcgg atttatctgc acaagaaggt cttttatttt
       61 agattctata tagtcaatat caatgattat aattttgtat ggattccctg cttccctggc
      121 agatatcaga gaagggagaa ttctttcagg ctcggaaaga gtctcgcttt ggagacccca
      181 ctgaacagta taatcatata taatgctgcg aaacccctct ctttgagaaa aaatcagcat
      241 ccttgaatct ttcagaagct ctgtatcata aatttcactg cggctttctt ccctcacttc
      301 tgcaggtatt tccgccagaa attctgcacc gtttggcata tttcgtgcgg tgattgttcc
      361 tccaaggctg tccaccatct ttttcacgat cgcaaggccg agtcccgttc ctccgtactt
      421 tcgagtaacg gacatatccg cctgaacaaa cggatcaaaa agatgcccag attcttcagg
      481 aagtccaatg cccgtatcac gcacagaaag actgatccag tctcttcctt ccctgctgaa
      541 cagatcagcc gtaatgagca cctgacctac tgcagtaaat tttacagcat tgctgaccag
      601 attgctgagt atctgtttca aaaaggaagg ctctgactga atgacaagat gcgagttcgg
      661 cttaaaatga aaattcagtt caatctgctt attatgagcc tttgcaacat atattcctgc
      721 aatatcctgc acgaactgtt cataattaaa atataatggt tccttgggca tcattcctgc
      781 ttccagtctt tcaaaatcaa gaatatcatt gatcagattc agcaggtgag cacttgcagt
      841 ttcaaaatta tgaataaaag aattctgctg agcagtgagt tccgtttcct tcagaagcga
      901 tgagaatcct atgattgcat tcaggggagt gcgaatttca tgactcatat ttgcgaggaa
      961 ttcggatttg gctctgcttg cctcctcggc ggcgagtttg gcctgcagaa gctcctgctc
     1021 cgcaattttt ctttcagtaa tatctgccaa tgttccatcc agacggaaag gatggcggtt
     1081 ttcatcaagt acgattctgg ccttgttgga aatccatttt actgacccgt ctccgcagat
     1141 gatcctgtat tctacatatg aattcccggt tttttccaga ttctgattta ttgtctgggc
     1201 aatatttctg tcatccggat gaatgacctt catccacagc gtgctgtcat ccatccattc
     1261 tttcactgaa tagccgtgaa gcttttcgac agacggagaa acaaaaagac ttttataatc
     1321 aggaagactt actgaccaga ttacatcctg aacctcattc aggacacttg ccagttttgc
     1381 ttcactttcc ttgactgcat tttttgcttt ggctgcatct
//
LOCUS       DAOQGJ010001303         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71224_length_1420_cov_3.521612, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001303 DAOQGJ010000000
VERSION     DAOQGJ010001303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71224_length_1420_cov_3.521612"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1420
                     /locus_tag="PK683_17370"
     CDS             <1..>1420
                     /locus_tag="PK683_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH10273.1"
                     /translation="IRSISRTGVSSVTVIFEDSVNVYFARQLVSERMKIAEADIPPEY
                     GRPEMAPVSTGLGDIYEFTLNSNSHTPEQLRTYMEWEIYRELKSVPGVIEINILGGEA
                     RQYQVLLDPKRMMSHNITVAEVLKRLKESNLNVGGGYIQKGNEQMVIRGEGKFNSLDE
                     IENTALKTERDGVPLYLGQVAKVKIGPALRFGVCTRDGKGEVVGATVMMLPGRNSREV
                     VRSVKERMELIISKLPPGMTVETFYDRTEFINKTLGTVFTNLAEGALLVLILLYFLLG
                     SWRGAVLVALAIPVTMAVTSIFMRAFGIVGNLMSLGALDFGLLVDGSIVILESVLTGF
                     VMGKTDLSESLTHKARIHRVQKIISESCAKVGRATVFSVAIIMLVYLPLMTLEGVEGK
                     MFRPMALTIAIALCCAMLYSVTAFPASISLLYKDVHIHESHFWVNLERQYSDLLKKAY
                     ENKRKVVWSALICFSVSVLCAMT"
BASE COUNT          385 a          297 c          362 g          376 t
ORIGIN      
        1 atccgatcca tttccagaac aggcgtgagc agtgtcactg taatttttga agacagcgtc
       61 aatgtatatt ttgcaagaca gcttgtttcg gaaagaatga aaattgcgga agctgacatt
      121 cctcccgaat acggaagacc ggagatggct cctgtatcga ccggtcttgg agacatctat
      181 gaattcactc tgaattccaa ttcgcacaca cctgagcagc tccgcacata tatggaatgg
      241 gagatttaca gggaactgaa atctgtaccc ggagtcattg aaatcaatat tctgggcgga
      301 gaagcgcgtc agtaccaggt tcttcttgat ccgaagagaa tgatgagtca caatatcact
      361 gtggcagaag ttctgaaacg gcttaaggag tccaatctga atgtaggcgg cggatacatt
      421 cagaagggaa atgagcagat ggtcattcga ggcgaaggaa aattcaactc gcttgatgag
      481 atcgaaaata ctgcactgaa gacggaaagg gacggagtcc cgctgtatct cggtcaggtg
      541 gcaaaagtga aaatcggtcc tgctctcagg ttcggagtat gcacgaggga cggaaaaggt
      601 gaggttgtag gtgcgacagt aatgatgctt ccaggcagga attccaggga ggtggtccgt
      661 tcagtcaagg aaagaatgga actcatcata tccaaacttc ctcctggaat gactgtggaa
      721 acattttatg acaggactga attcataaac aagactctcg gcactgtttt tacgaatcta
      781 gcagagggtg ctcttctcgt tctcattctg ctctacttcc tcctcggttc ctggagggga
      841 gcagtccttg tggcacttgc aattccggtg acaatggcag tcacttccat ttttatgaga
      901 gctttcggga tcgtaggaaa tctgatgagt ctaggtgcac ttgacttcgg tcttctcgtc
      961 gacgggtcca tcgtcattct ggaatcggtt ctcaccggtt ttgtgatggg aaagactgac
     1021 ctcagtgaat cactgactca taaggcaaga atacataggg tgcagaagat tatttctgaa
     1081 agctgtgcaa aagtgggaag ggcaactgta ttttctgtgg caatcatcat gctcgtatat
     1141 ctgcctctga tgacattgga aggagtagaa ggaaaaatgt tccgtcctat ggcgctcacc
     1201 attgcaattg cactctgctg cgcaatgctt tattccgtaa ctgcattccc tgcttccata
     1261 agccttctgt acaaggacgt gcatattcat gaaagtcatt tctgggtaaa cctggaaagg
     1321 cagtattcgg atcttctgaa aaaggcatat gagaataaaa gaaaggtcgt ctggtctgca
     1381 ctcatatgtt tttccgtttc cgtgctctgt gcaatgacac
//
LOCUS       DAOQGJ010001304         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71241_length_1420_cov_3.076923, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001304 DAOQGJ010000000
VERSION     DAOQGJ010001304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71241_length_1420_cov_3.076923"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /gene="ilvN"
                     /locus_tag="PK683_17375"
     CDS             complement(<1..137)
                     /gene="ilvN"
                     /locus_tag="PK683_17375"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNH10274.1"
                     /translation="MKHTISILVNNHPGVMSHVSGLFTRRAYNIDSIAVGVTDNPEVS
                     S"
     gene            complement(137..>1420)
                     /locus_tag="PK683_17380"
     CDS             complement(137..>1420)
                     /locus_tag="PK683_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH10275.1"
                     /translation="RHFEEACRIALGGRPGPVLLDFPKDVQIKSTSKLKADRLKIAPH
                     HYEKPQIQGSLEKFADALNRAKRPLLYVGGGAINANAAKEIKELAEKAEIPVTMTLMG
                     LGAFPGTHPLSLGMLGMHGTAYANKAVLECDYILNLGARFDDRVAKLRDFAPQAVRAH
                     IDVDVSEFNKRVNVDYSLHGDLKDVLKKLIPLVKKNADRKVWTDRTSELKKSHPLEFD
                     SSGDTVKPQDFLHRLYKKTEGKAIISTDVGQHQMWAAQYYLQDEPNNWLTSGGLGTMG
                     YGLPAAIGAKFGRPDKMVICVSGDGSIQMCIQELATISMYKLGVKILIFNNNFLGMVR
                     QWQELFYEERFSHSEWNYNPDFVKLGEAYGIPGMKIQSKSEIDKAIEFFVKDDSACIL
                     EVMIPAEEKVFPMIPAGKSQTDMIEYKDLQKLRGK"
BASE COUNT          324 a          360 c          297 g          439 t
ORIGIN      
        1 atggaagaaa cttcaggatt gtcagtgact cctactgcta tagaatcaat attatatgct
       61 cttctagtaa aaagcccgga cacatgactc atgacgcctg ggtggttgtt gacaagtatg
      121 ctgattgtat gtttcattat tttcctctca gcttttgaag atctttgtac tcaatcatat
      181 ccgtctgtga ttttccggcc ggaatcatag gaaagacttt ttcttctgca ggaatcatca
      241 cttccagtat gcaggcagaa tcatccttga cgaagaattc tattgccttg tctatttcac
      301 tcttggactg aattttcatc cccggtattc cgtaagcttc tccgagtttc acaaaatcag
      361 ggttgtagtt ccactctgaa tgggaaaatc tttcctcata gaacagttcc tgccactgtc
      421 tgaccattcc gagaaaattg ttgttgaaga tcagtatctt gactcccagt ttatacatgc
      481 tgattgtagc cagctcctga atgcacatct gaatggatcc gtcacctgaa acgcagatga
      541 ccatcttgtc cggccttccg aattttgcgc cgattgcggc aggcagtcca tatcccatcg
      601 tgcccagtcc gcccgatgtg agccagttgt tcggctcgtc ctgcagatag tactgcgccg
      661 cccacatctg gtgctgcccc acatcagtgc tgatgattgc cttgccttca gtttttttgt
      721 acagtctgtg cagaaagtcc tgaggtttaa ctgtatcccc tgaagaatca aattccagcg
      781 gatgggattt cttcagttca gaagttctgt ctgtccagac ttttctgtcc gcattttttt
      841 tgaccagagg aatcagtttt ttcagaacat ccttcaggtc gccgtgcaga ctgtaatcga
      901 cattcactct tttattgaat tcagatacat ccacatctat atgtgctctc acagcctgag
      961 gggcaaagtc tctcagtttg gctaccctgt cgtcgaacct tgctcccaga ttcagaatat
     1021 agtcgcattc cagaactgcc ttgtttgcgt atgcggttcc atgcattccc agcattccga
     1081 gggaaagtgg atgtgtgccc ggaaatgctc ccagacccat cagcgtcatt gtgacaggaa
     1141 tctcggcctt ctcagccagc tccttgattt cctttgcagc atttgcatta atcgctcctc
     1201 ctcctacata aagaagaggc cgtttggccc tgttcagtgc atctgcaaat ttttcaagac
     1261 tgccctgaat ctgaggtttt tcataatgat gaggagctat tttcagtctg tctgccttca
     1321 gttttgaagt ggacttgatc tgaacatctt tcggaaagtc aagaagcacg ggccccggtc
     1381 ttcctccgag agcaatcctg caggcttcct caaaatgcct
//
LOCUS       DAOQGJ010001305         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71396_length_1418_cov_3.667645, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001305 DAOQGJ010000000
VERSION     DAOQGJ010001305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71396_length_1418_cov_3.667645"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..>1418)
                     /locus_tag="PK683_17385"
     CDS             complement(189..>1418)
                     /locus_tag="PK683_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10276.1"
                     /translation="TRPDGLLIYGISALSVLFLEYNIAITDQSKSKFYLKIFEVAKRV
                     FIANFFFAFVFIPYWMARWSYYGKFFPNTFYAKSGNLSYWSQGAFYLYTYFVSYWVFL
                     ILPILMLYVFIGKKYRNTGKDVGSTIALITVFSWSFYLFKLGGDFMFARFIIPITPLL
                     FLLTEKYLLCILFNSKNEIIARFLKMTYAPTFISLFFIVTVLLRYDFYKGEKYPNIRG
                     ISEEYKVYPIEFVHNMQKKAAQHKTALVESKAKIAILGAEALLAYYWNPVETIEAVSG
                     LTDSFIASQEVKERGMPGHEKEAPIEYLRDRRVNLRLAKPPSDRDHGFNQISMDGMIG
                     YGEIVAYVPSVMLSLKKHPGIQFKDFNEFLKEYISRHPSKETVTKDLPVFKEYYFRYA
                     PEDKRLSLLEKIAQGAK"
BASE COUNT          447 a          269 c          259 g          443 t
ORIGIN      
        1 tatcagcccg tcagtaagac ttgacagact cactgcgctt gtcagccgaa acgacaatat
       61 caaaaaattc attgtccata tttttagcat tgtactgatt tcttttctac tttactctct
      121 tttgtgtaag gtgagctact catttataac tactcaaatt tattttccgc tgagttgttg
      181 catttatttt atttcgcccc ttgagcaatt ttttccaata aggaaagtct tttgtcctca
      241 ggagcatatc tgaaatagta ttctttgaag acaggtaagt cttttgtaac agtttctttc
      301 gatgggtgtc tgcttatgta ttccttaaga aactcattaa agtccttaaa ctgaattcct
      361 ggatgtttct tcaatgacag cataacgcta gggacatatg caacaatttc cccatagcca
      421 atcattccat ccattgaaat ttgattgaat ccgtgatccc tgtcgctggg aggtttggct
      481 agcctgagat ttactcttct gtctcttaaa tactctattg gtgcttcttt ttcatgtcca
      541 ggcattcctc tctctttgac ctcttgagat gcaataaaag aatcagtcaa tccagaaaca
      601 gcttcaatag tttcaaccgg attccagtaa taagccaaaa gagcctcagc tcctaaaatg
      661 gcaatttttg ctttggattc aactaatgca gttttatgct gtgcagcctt tttttgcata
      721 ttgtgcacaa attcgatagg ataaacttta tattcttctg aaataccgcg aatattcgga
      781 tatttctccc ctttataaaa gtcgtacctc aatagtaccg tgacaataaa aaacagagaa
      841 atgaatgtag gggcataagt cattttcaag aaccgtgcaa ttatttcatt tttggaattg
      901 aacagaatgc agaggagata cttttcggtc agaaggaaga gaaggggggt gatcgggatg
      961 ataaatctag cgaacataaa gtctcctcca agtttgaaca gatagaaaga ccaggagaaa
     1021 acagtgatta gtgcaatggt gctcccaaca tccttgccag tattcctgta tttttttcca
     1081 ataaaaacgt aaagcatcag tatcggaagt atcagaaaaa cccagtaaga aacaaaatag
     1141 gtataaagat agaaagcacc ttgactccaa taggaaagat tgcctgattt tgcgtaaaat
     1201 gtattgggga aaaatttacc ataataactc catctggcca tccagtaggg aataaaaaca
     1261 aaggcaaaga agaagttggc aatgaaaact ctctttgcaa cttcgaaaat ttttaaataa
     1321 aacttgcttt ttgattggtc ggtaatagca atgttatact ccagaaacag gactgaaaga
     1381 gcggagattc catatatcag aagaccatcc ggtcttgt
//
LOCUS       DAOQGJ010001306         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71411_length_1418_cov_3.002201, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001306 DAOQGJ010000000
VERSION     DAOQGJ010001306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71411_length_1418_cov_3.002201"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="proB"
                     /locus_tag="PK683_17390"
     CDS             complement(<1..832)
                     /gene="proB"
                     /locus_tag="PK683_17390"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNH10277.1"
                     /translation="MKQRRSEFTSALKKAERIVIKVGSARVSGDEHEINDFLFSLVGD
                     IRELRDQGKDIILVSSGAIAQGRKVLSMYRNEKTGSSVREKQALAAIGQSLLMKMYSH
                     FFSSVNIPIAQILFGSNDLSAESGYSNLKNTFEKLSEWKVLPIINENDSVATEELKLG
                     DNDILSALVAILVKADLLVILTGVDGFQKDGKTVPFLEEITEEDLSFAKGPEGPGTGG
                     MNTKLRAAGILLKAGISTAVISGKEKNCIRNFLNSNTGGTLVSGKMQKKSLSEKEIKK
                     L"
     gene            complement(813..>1418)
                     /locus_tag="PK683_17395"
     CDS             complement(813..>1418)
                     /locus_tag="PK683_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002723975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNH10278.1"
                     /translation="AQDGEEGESKDILLTLKLLADVGLVGLPNAGKSTLLSRITHAHP
                     KIASYAFTTLSPNLGVVDREDSFRRYTIADIPGIIEGASKGHGLGLSFLRHVERVTGI
                     IYMFDATSLDIEEEYKMLQNELKTYSKTLVKKKSIVVLNKSDLVDDQDFLQEMVKNLK
                     IKEEIICISAEKGTGLDILLNAIDRVFFGAKKRKKSEAKKI"
BASE COUNT          347 a          349 c          277 g          445 t
ORIGIN      
        1 aaagtttttt aatttccttt tcactcaagc tttttttctg catttttcct gataccaatg
       61 ttccgcccgt attggaattt aagaaatttc taatacagtt tttttccttt cctgaaatca
      121 cggcagtgga aatacctgcc ttaagcagga tgcctgccgc cctgagcttg gtattcattc
      181 ctccggttcc ggggccctca ggtccctttg caaaggagag atcctcttca gtgatttctt
      241 cgagaaacgg aactgtcttt ccgtctttct gaaatccgtc gacaccagtc agaataacga
      301 gaaggtccgc cttcacaagg attgcaacga gagcagaaag aatgtcgttg tctcccagtt
      361 taagttcttc cgttgcgaca gaatcatttt cattgatgat cggaagtacc ttccactcgg
      421 aaagtttttc aaaagtattc ttcagattcg aataaccgct ttcagccgaa aggtcatttg
      481 atccgaaaag gatctgagca atcggaatgt ttacgctgga aaaaaaatgt gaatacattt
      541 tcattaaaag actctgtccg atggcggcaa gtgcctgttt ttccctgaca ctgcttccag
      601 tcttctcgtt tctgtacata gaaagaacct ttctgccctg tgcaattgcc ccgctcgaaa
      661 caaggataat gtcctttccc tgatccctga gttctctgat atcgcccaca agactgaaca
      721 gaaaatcatt aatctcatgt tcatctccgc tgaccctggc tgaacctacc ttgattacga
      781 tcctctcggc ctttttcagg gccgatgtaa attcagatct tctttgcttc actttttttc
      841 ctcttttttg caccgaagaa aactctgtca attgcgttca ggagaatatc gagacctgtg
      901 cccttttctg ctgaaataca gattatctct tcctttattt tcaagttttt taccatttcc
      961 tgcagaaaat cctgatcgtc cacaagatcg gacttgttca gaacgacgat cgatttcttt
     1021 tttacaagag ttttgctgta agtcttcagt tcgttctgaa gcattttgta ctcttcttct
     1081 atgtcgagag acgttgcatc aaacatatat atgatccctg tcactctttc tacatgtctg
     1141 agaaaggaaa gccccagtcc gtgtcccttg cttgcaccct cgatgattcc gggaatatct
     1201 gcaatggtat agcgcctgaa actgtcttcc ctgtccacaa cgcccagatt gggactcagc
     1261 gtagtaaatg cataggaagc aattttggga tgagcatgag tgattctgct gagcagtgta
     1321 gacttgcctg cattagggag accgacaaga cccacgtcgg ccagaagttt cagagtgagc
     1381 agaatatcct tggactcgcc ctcctctcca tcctgtgc
//
LOCUS       DAOQGJ010001307         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71435_length_1418_cov_2.364637, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001307 DAOQGJ010000000
VERSION     DAOQGJ010001307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71435_length_1418_cov_2.364637"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PK683_17400"
     CDS             complement(<1..747)
                     /locus_tag="PK683_17400"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH10279.1"
                     /translation="MNPWTFTERIGKYILMLLHDVGEFGGLYFAAGFSPLYEGTEARK
                     IIFESLVKQIYFTGVQAVRPIMIVSLLAGGIIIIQTTSQLPKVGAESMLGNILVLAVV
                     REFGPLAVSLIIIGRSATAVTAEISEMKVSGQFAHLLASGINPASYLFAPRFYGMIYS
                     MLGLQIIFTATAIFGGFLMARAITFVSLPYLLAAFFNRLTWADILGLILKSCFLASAI
                     SVISIREALKVKSSPTEIPQAASRTVVSTIL"
     gene            complement(749..>1418)
                     /locus_tag="PK683_17405"
     CDS             complement(749..>1418)
                     /locus_tag="PK683_17405"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNH10280.1"
                     /translation="LLATIPLFYSRIAYLPTILGFTGAGIILSMKDVTLNFIGWFFIH
                     SNSGFTVGDRIEIQGTKGDVVNIGMMRFTLVELSHDENNEQSTNRLVHIPNHKVISEK
                     ILISRSEMDYVWDELKISITVDSDWEKAETVCNSILYEVFTQYVNHEELQEKVRKLSE
                     HYLLKIGKTSPITYTVIEEHVIRLSLRYLVRVHEKRNMRSDLSKKVLKQFKKFPKIRI
                     YVPD"
BASE COUNT          399 a          306 c          309 g          404 t
ORIGIN      
        1 cagaattgtg ctgaccaccg ttcttgaagc agcctgggga atttctgtag gggaactttt
       61 gactttcagg gcttccctga tggagatgac tgaaattgca gatgcaagaa agcagctttt
      121 cagaatcagt ccaagaatat cagcccatgt gagcctgttg aaaaatgctg caagaaggta
      181 gggcagactc acaaaagtga tggcacgtgc catcaggaac cctccgaaaa ttgcagttgc
      241 agtgaatata atctgcagtc cgagcattga gtaaatcatt ccgtagaatc tgggagcaaa
      301 cagataggat gcaggattga tccctgatgc cagcagatga gcaaactgac cgcttacctt
      361 catctctgaa atttctgcag tgacagcagt ggcacttcgt ccaatgatga tcagactgac
      421 agcgagagga ccgaactctc tcactactgc cagaacaaga atatttccga gcatactttc
      481 tgcaccgacc ttaggaagct gacttgtagt ctgaataatg atgattcctc ctgcaagaag
      541 cgaaacaatc ataatcggtc tgaccgcctg cacgccagta aaataaatct gttttacaag
      601 ggactcaaaa atgatcttcc tggcttcagt tccttcgtac agcggggaaa atccggctgc
      661 aaaataaagt cctccgaatt ctccgacatc atgcaatagc attaaaatgt atttaccgat
      721 tctttctgta aaagtccagg ggttcatttt aatcaggaac gtaaattctg attttaggaa
      781 attttttaaa ctgcttcagt actttcttgg aaaggtcgga tctcatattt cttttttcat
      841 gcactctgac aagatatctg agcgaaagac ggatcacatg ttcttcgatg acagtgtatg
      901 tgatcggtga ggtttttccg attttgagaa ggtaatgctc tgagagtttt ctgacctttt
      961 cctgaagttc ctcatgattg acatactgcg taaatacttc atacaggatg gaattgcata
     1021 cagtttctgc cttctcccag tcagaatcca ctgtgatgct gattttcagt tcatcccaga
     1081 catagtccat ttcggatctg gaaatgagga ttttttctga aatgacttta tggtttggaa
     1141 tatgcaccag tctgttcgtg gactgttcgt tgttttcatc atggctgagt tccactagag
     1201 taaaacgcat catccctatg tttaccacat ctcccttcgt tccctgaatt tctatcctgt
     1261 cgccgacagt gaatcctgaa ttgctgtgaa taaaaaacca gcctatgaaa ttcagagtca
     1321 cgtccttcat tgaaagaatg atgcccgccc cggtaaatcc cagaattgta ggaaggtagg
     1381 ctatccggct gtaaaaaagg ggaatggttg caaggagc
//
LOCUS       DAOQGJ010001308         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71495_length_1417_cov_2.979442, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001308 DAOQGJ010000000
VERSION     DAOQGJ010001308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71495_length_1417_cov_2.979442"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..>1417)
                     /locus_tag="PK683_17410"
     CDS             complement(32..>1417)
                     /locus_tag="PK683_17410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNH10281.1"
                     /translation="TSPTLALYTKFKVGSADETPEIAGTAHLLEHMLFKGTENIGTSD
                     FKSERKYYTLMKFTGSELDSLKLEKRAYAEKGKEVPAELEKRIGVLTRRLKTIEEAEK
                     KYIISSEDSFIYEQNGQAGFNAYTSHDVTNYQIKLPSNRLELWAKMESDRLKNPILRE
                     YFTERDVIMEERRMRVENRGFGILREKYLAAAFEQSPYRRPVIGYESNIPFLNIYETE
                     NFFRTYYSPDNMVIGLVGDLDFEEAESVIKKYFGDLKPSGNRRKELRVKERFNMGERR
                     VSFKYTGGSTLIMGWNKPSFPHIEHSAFELMDAILTKGAESRLYKSAVLREKIASGVS
                     VWASDPGERYSNLFSVIANLNSDADPARMEALVWEEIEKLKNGEITEEEIQKAKNKVT
                     ADFLRDVDSNASLADGLTYYELIGGNWEEMFLIYDRFNNVKKEDIQRIAKKYLVKENM
                     TVGFLDARGTK"
BASE COUNT          344 a          321 c          258 g          494 t
ORIGIN      
        1 gttattaatg taattataat gataaaatat ttcattttgt acctcttgca tccaaaaaac
       61 cgactgtcat attttccttg accaggtatt tttttgcgat cctctgaatg tcttctttct
      121 tgacattatt gaatctgtcg tatatcagga acatttcttc ccagtttcct ccgatcagct
      181 catagtatgt aagaccgtcg gcaaggctgg cgttgctgtc cacatcgcga agaaaatcag
      241 ccgtgacctt gttttttgct ttctgaattt cttcttcagt gatttctccg ttcttcagtt
      301 tttcaatttc ttcccagaca agtgcttcca ttcttgcagg atctgcatct gaattcagat
      361 tggcaattac agaaaaaaga ttcgaatatc tttctccagg gtcagaagcc cagacagaga
      421 ctccggatgc aattttttcc ctgagtacag cggatttgta aagtctgctc tcagctcctt
      481 tagtcagaat tgcatccatc agttcaaatg cactgtgttc gatatgcggg aaagagggtt
      541 tgttccagcc cataatcaga gttgagccgc ctgtatattt aaaactgacc ctcctttctc
      601 ccatattgaa tctttctttg acacgcagtt cctttcttct gtttccggaa ggcttaaggt
      661 cgccgaaata ttttttaatg acagactcag cttcttcaaa atccagatcg cccacaagcc
      721 caatcaccat attgtccgga ctgtagtaag tcctgaaaaa gttctctgtt tcgtaaatgt
      781 tcagaaaagg aatgtttgat tcatatccaa tcactggcct tctgtatgga ctctgttcaa
      841 atgctgctgc aagatatttt tcccgaagaa ttccgaatcc tctgttttcc actctcattc
      901 ttctttcttc cataatcaca tcccgttctg taaaatattc tctgaggata ggattcttca
      961 gtctgtccga ttccattttc gcccagagtt ccagtctgtt ggaaggaagc ttgatctggt
     1021 aatttgtgac atcgtgcgag gtgtatgcat taaatccagc ctgaccgttc tgttcataaa
     1081 taaacgaatc ttctgatgaa atgatatatt tcttttccgc ttcctctatt gtcttaagtc
     1141 ttctggtgag aacgcctatc ctcttttcaa gttcggccgg aacctctttt cctttttcag
     1201 cataagctct tttctcaagt ttaagtgaat ccagttcaga accggtaaat ttcataagcg
     1261 tatagtattt tctttctgat ttgaaatcgc ttgttccgat attttcagtg cctttaaaca
     1321 gcatatgctc cagaaggtgt gctgttcctg cgatttcagg ggtctcatca gctgagccca
     1381 ctttaaactt ggtgtaaagt gccagagtag gcgaggt
//
LOCUS       DAOQGJ010001309         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71596_length_1416_cov_2.564291, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001309 DAOQGJ010000000
VERSION     DAOQGJ010001309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71596_length_1416_cov_2.564291"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..412
                     /gene="ybeY"
                     /locus_tag="PK683_17415"
     CDS             29..412
                     /gene="ybeY"
                     /locus_tag="PK683_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNH10282.1"
                     /translation="MKNCEKAVNDTVRLKDFFLELNIVSDTEIRQLNLEKRKKDRATD
                     VLSFPLYNPDLKIPVQNLGQIVISYDTMKKQAKEIGHSLKEEFYRLLVHGILHLLGYD
                     HEVSPEEEKKMQKKEDWVLRLIFQG"
     gene            496..>1416
                     /locus_tag="PK683_17420"
     CDS             496..>1416
                     /locus_tag="PK683_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10283.1"
                     /translation="MIKGILVLLLSICLSAEEGNHSGFGYPLNTQNVEYSPVISPNSR
                     YIVFQSNRPGGKGGMDIWLSEDKNFEDRTGKPEWAPPVNFKELNTGDFEGPFSILFDE
                     NGKPIEIYFTGRRESSIKRDGLEGLNIYLVKNLTRKNLSTDRWSKPEHLMRVNSNFDD
                     KMPSISPDGKTLVFSSNRPGGYGGFDLWASSRDMKTDSWSEAVNLGSRANSGSNEIMP
                     YYHYDGMALYFSSDRNDDNHKFHFYHASLEEPGLILSDEKAESVYEKRDRIPRISELK
                     KLSRPYNSAFDDEGISFSHDGLWVYFASNRP"
BASE COUNT          457 a          283 c          303 g          373 t
ORIGIN      
        1 cagacttccc gactcaaaaa aaaacataat gaaaaactgt gaaaaggcag taaacgatac
       61 agtccgactg aaagattttt ttctggaatt gaatatagta tccgatacgg aaatccgtca
      121 gctcaatttg gagaaaagaa aaaaagacag ggcgacggat gtgctttctt ttcctctcta
      181 caatcctgat cttaaaatcc ctgtccagaa tttaggacag attgtgattt cctatgatac
      241 aatgaaaaaa caggcaaagg aaattggtca ttccttgaag gaagagtttt acagactttt
      301 agtccacgga attcttcatc ttctgggtta tgatcatgag gtttcccctg aagaggaaaa
      361 aaagatgcag aaaaaggaag actgggtact tagactgatc tttcaaggtt aaaaaatctc
      421 ctttcatctg aatttttagc tgaaaaacac aaaaaagctt taaaaaaaag ggcttttcag
      481 agaaaagaat attgcatgat taaaggaatt ctagttttac ttctgtccat ttgtctctct
      541 gccgaagagg gaaatcattc cgggttcgga taccctctga atactcagaa tgtagaatat
      601 tcaccggtca tatctccaaa ttccagatat attgtctttc agtccaaccg tccaggcgga
      661 aaaggcggaa tggatatctg gctctcagaa gacaaaaact ttgaagacag aaccggaaaa
      721 cctgaatggg ctccgcctgt caatttcaag gaactgaaca ccggtgattt tgaaggccct
      781 ttcagcattc tttttgatga aaacggaaaa cctatagaaa tctattttac tggaagaaga
      841 gagtcatcca tcaaaagaga cggtctggaa ggtctgaaca tctatcttgt aaagaacctg
      901 acgaggaaaa atctttccac agacagatgg tcgaaaccgg aacacctgat gagggtcaat
      961 tccaatttcg atgataagat gccttccatt tcgccggacg gaaagactct ggtgttcagt
     1021 tccaacagac cgggcggata cggaggattt gatctctggg cttcaagcag ggatatgaag
     1081 accgattcct ggtcggaggc tgtgaatctg ggaagcagag cgaactccgg ttctaatgaa
     1141 attatgccat attatcatta tgacgggatg gcattgtact tcagttccga cagaaacgat
     1201 gataatcaca aatttcattt ttatcatgca tctctcgaag agcccggcct tattctgtcg
     1261 gatgagaaag cagaatcagt atatgaaaaa agagacagga ttccccgtat ttccgaactg
     1321 aaaaaacttt cccgtccgta caacagtgcc tttgacgatg agggcatcag tttttcccat
     1381 gacggactct gggtctactt tgcatccaac cgtccg
//
LOCUS       DAOQGJ010001310         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71651_length_1415_cov_3.210294, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001310 DAOQGJ010000000
VERSION     DAOQGJ010001310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71651_length_1415_cov_3.210294"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1346
                     /locus_tag="PK683_17425"
     CDS             78..1346
                     /locus_tag="PK683_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10284.1"
                     /translation="MKLIETVFQYGNDPAVRLNYSGLYFFEGTPPKQDSLVFLNLPGL
                     EKLWPEEEVRNWFAQYMKKESFSRKDLIRISADLWRPAKIEDLLEAFNYYNYNITSYF
                     SQSEFQRFLSDCVPRLISEMKYILHIKDEKRKLSEAKIFQPKFARHSAIMNLTSSKIS
                     QDIPKWEDEIERKILTELKSKNASDEELLKEGNQLSSANIRREVLRLFAERQTAKKLS
                     GKELLEKILIIQNYTVLRNDTVFTGHDFWRILQPHLQFEANTFGETFDITDAEETTPV
                     LNWDFINAKRIGPVVPGRSALFIPGVPNVYIRGERKLKFKVQKAGGKRSLFQNTLTLE
                     TDRNSLSTLLLETEKIIALSSLAPKQAVRLADHLNLSRDHIVYKTAEEAEWNRANARI
                     LSDLLIELLAGIDSDTARRLVREEKRSNRR"
BASE COUNT          464 a          275 c          307 g          369 t
ORIGIN      
        1 attcagtgac agtgctgccg aaaggtttac agaggttttg gacagactga gatattttca
       61 actgatggcg gaatgatatg aaactgatag aaactgtatt tcaatatggg aatgatccgg
      121 cagttcgact gaactactcc ggtctgtatt tctttgaagg aacacctcct aaacaggact
      181 ctcttgtctt tctgaatctt cccggactgg aaaaactctg gccggaagaa gaggtacgaa
      241 actggtttgc acagtacatg aagaaggaaa gtttttccag gaaggatctg atccgaattt
      301 ctgcagacct ctggagaccg gccaaaatag aagacctttt ggaagcattc aattattata
      361 attataatat cacatcctat ttttctcagt ctgaatttca gagatttctt tccgactgtg
      421 tgcctaggct gatcagcgag atgaaatata ttcttcacat taaggatgag aaaagaaaac
      481 tttctgaagc aaagattttt cagccaaagt ttgcaagaca ctcagccata atgaatctga
      541 cttcatcaaa aatcagtcag gacattccaa aatgggagga tgaaattgaa agaaagattc
      601 tgactgaact gaagagcaaa aatgcatctg atgaagaact gctcaaagaa ggaaatcagc
      661 tttcttccgc aaatatccgc agagaggttc tgcgtctctt cgcagaaaga cagactgcaa
      721 aaaaactttc cggaaaggaa cttttagaga aaatactgat aatccagaat tatacagttt
      781 tgagaaatga taccgttttt accggacacg atttctggag aatccttcag cctcatctgc
      841 agtttgaagc aaacactttc ggtgaaacct ttgatatcac tgatgcagaa gaaacaactc
      901 cagtgctgaa ctgggacttc atcaatgcaa aaagaatcgg tcctgttgtt ccagggagat
      961 ccgccttatt tattccggga gttccgaatg tgtacatacg gggagaaaga aagctcaaat
     1021 tcaaggttca gaaagcggga gggaaacgca gtctttttca gaatacactg actttggaaa
     1081 cagacaggaa ttcactcagt acacttcttc tggaaacgga aaagatcatt gctctttcat
     1141 ctcttgcacc aaagcaggca gtgcgacttg cagaccatct gaatctttcg cgggaccata
     1201 ttgtatacaa gactgcagaa gaagcggaat ggaacagagc aaatgcaaga attctctctg
     1261 atcttctgat tgaacttctg gcagggattg attcagatac tgcgagaaga cttgtcagag
     1321 aagagaaaag atccaaccga cgctgatatg tgcagtatac aaaaaagccc gcgacaagtg
     1381 ccgcaggctt ttaatgtttt ttaattacga aaaat
//
LOCUS       DAOQGJ010001311         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71681_length_1415_cov_2.338235, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001311 DAOQGJ010000000
VERSION     DAOQGJ010001311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71681_length_1415_cov_2.338235"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /gene="lysS"
                     /locus_tag="PK683_17430"
     CDS             <1..1351
                     /gene="lysS"
                     /locus_tag="PK683_17430"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002188823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNH10285.1"
                     /translation="PLYRLGGRLHSKRVMGKASFGHIKDKAGSIQLYAARDDLGEEKY
                     GIFKSLDLGDQIGIEGYLFRTQKGEISIHVTDLHLLAKCIRPLPVVKEKDGVVFDAFS
                     DKEQRYRMRYVDLTVNDHVRETFIMRSRIVQEVRNFFIGEGFLEVETPMMQPIAGGAA
                     ARPFITHHNTLDMDLYLRIAPELYLKRLIVGGMDRVFEVNRNFRNEGISLKHNPEFTM
                     MEAYMAYGDMETMLKLTERLFVHIATATGKGLKIPYGEHKVDLTPPWKRITYVDIIKE
                     YSGIDFSQVKTLAEAKEKAKKAGVDAEECVSIWKVADEVFSQKAEPNLIQPVFVTDYP
                     KELSPLAKSREDNPAYVERFEPYIVGREIGNAFSELNDPFDQKARFEEQVAQREAGDD
                     EAFMMDHDFIRALEYGMPPTGGLGIGIDRLVMLFTDSPSIRDTIFFPLMRHQEHFQS"
BASE COUNT          433 a          291 c          358 g          333 t
ORIGIN      
        1 gcctctttac agactcggag gaagactgca ttccaagaga gtgatgggaa aggccagctt
       61 cggtcatatc aaggacaagg caggaagcat tcagctttat gcagccagag atgatctggg
      121 agaagaaaaa tacggaattt tcaagtctct ggatctggga gatcagattg gaattgaagg
      181 gtatcttttc agaacgcaga agggagaaat ttccatccat gtgactgatc tgcatcttct
      241 cgcaaaatgc atacgtcctc ttccggtcgt aaaagaaaaa gacggagtcg tctttgatgc
      301 attcagcgac aaggaacaga gatacagaat gagatatgtg gatctgactg tgaatgatca
      361 tgtcagagaa acattcatta tgagaagcag gattgtgcag gaggtcagaa acttcttcat
      421 cggcgaagga tttttggaag tggaaacccc gatgatgcag ccgattgcag gcggggccgc
      481 agcccgtccg ttcattactc atcacaatac tctggatatg gatctttact taaggattgc
      541 accggagctt tacttaaaaa gactgattgt gggcggaatg gacagagtgt ttgaagtgaa
      601 tagaaacttt cggaacgaag gaatctctct caagcataat cctgaattca caatgatgga
      661 agcctatatg gcctacggcg atatggaaac aatgctgaaa ctgacagaaa gactgtttgt
      721 gcacattgcc actgcgaccg gaaaaggact gaagattccc tacggtgagc acaaggtgga
      781 cctcactccc ccatggaaaa gaatcactta tgtggacatc ataaaggaat attcaggaat
      841 tgacttcagt caggtcaaga cgttggctga agccaaagag aaagccaaaa aagcaggagt
      901 ggatgcagaa gaatgcgtca gcatctggaa ggtggcagat gaggtattct cgcagaaagc
      961 agaacctaac ctgattcagc ctgtattcgt gactgactat cctaaggaac tttcccctct
     1021 ggcaaaatcc agagaagaca accctgccta tgtggaaaga tttgaaccct acatagtagg
     1081 ccgtgaaatc ggaaacgcat tctccgagct gaatgatccg tttgatcaga aagccagatt
     1141 cgaagaacag gttgctcaga gagaggccgg cgatgatgaa gcattcatga tggatcatga
     1201 ttttatcagg gctttggaat atggaatgcc tcccactgga ggactgggaa tcggaataga
     1261 cagacttgtc atgcttttca ctgacagtcc ttcaatccgg gatacaatct tcttcccgct
     1321 gatgagacat caggagcatt ttcaaagctg actgagattt acaaaaattt gcgcacgctg
     1381 ccttttcgga gcgtgcttca tattccgcac aaacc
//
LOCUS       DAOQGJ010001312         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_71843_length_1413_cov_2.952135, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001312 DAOQGJ010000000
VERSION     DAOQGJ010001312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71843_length_1413_cov_2.952135"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK683_17435"
     CDS             <1..222
                     /locus_tag="PK683_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10286.1"
                     /translation="LRYDQKASPSKMISSITTLGYLTMAVLYHWPFSGWRRSYKGLKE
                     LYRSNPEEAVKAYNRFTIKRGLTTRIGGG"
     gene            284..>1413
                     /gene="asnB"
                     /locus_tag="PK683_17440"
     CDS             284..>1413
                     /gene="asnB"
                     /locus_tag="PK683_17440"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNH10287.1"
                     /translation="MNSIRHRGPDDSGIFENKDCCLGHRRLSIIDIQNGKQPFISDDG
                     RYSLVFNGEIYNYLELRQMLVSKGYNFHTFSDTEVLLNMYIEFGRKSLDYLNGMFAFA
                     VYDSSESSLFLARDHFGIKPIYYYSDSECLIFASEIKALLQHPRIKAQADWNSINEYL
                     VLQMVLGKETLFKNIHKLEPATYMTAVNGRIKEERRYWDLQYQVDDEKSMDRFSDELL
                     VLLESSLSIQMRSDVPVGAYLSGGLDSSIVATLAAKTYPGSLKTFTGGFKDSAQYDET
                     LYASVVSQSIGSEHHEIFPTYHDFLENFESLVYFMDEPVAGPGMFPQYMVSKLASKHV
                     KVVLGGQGGDEIFGGYARYAVAYLEQCLKGAILETQEEENHVV"
BASE COUNT          409 a          240 c          326 g          438 t
ORIGIN      
        1 ctgcgatatg atcagaaggc aagtccgagc aaaatgatca gcagtattac taccttaggt
       61 tatcttacaa tggcagtact ttatcattgg cctttttctg gatggagaag aagctataag
      121 ggacttaaag aattgtaccg tagtaatccg gaagaagcag tgaaggcata taatcggttt
      181 actatcaaac gcggattgac tacacgaata ggcgggggct gaaatgtgcg ggtttgccgg
      241 aatatttgga cttcttagtc aaaaagaata cctactgaaa atgatgaatt ctatcagaca
      301 cagaggtcct gacgattccg gtatttttga aaataaagac tgttgtttag ggcacagacg
      361 tctgtctatt atagatattc aaaatggaaa acagccgttt atatctgatg atggacggta
      421 ttcacttgta ttcaacggag agatttataa ttatcttgaa ctgagacaaa tgctcgtttc
      481 caagggatac aatttccata cattttctga tacagaagta cttctgaata tgtatatcga
      541 atttggcaga aagtctttgg attatctgaa tggtatgttt gcctttgcag tttatgactc
      601 ttctgagtct tctctttttt tggctcgtga ccattttggt attaaaccta tatactatta
      661 cagtgacagt gagtgtctga tatttgcttc tgaaatcaaa gcactgctgc agcatccgcg
      721 tataaaagca caggctgact ggaacagtat aaatgaatac ttagttcttc agatggtatt
      781 gggcaaagag acgctgttta agaatatcca taaactcgag ccagccacat atatgactgc
      841 agtgaatggc aggataaagg aagaaaggcg ttactgggat cttcagtatc aggtagatga
      901 tgaaaagtct atggatcgtt tttctgatga actattggtt cttttggaaa gttctctctc
      961 cattcaaatg cggtcagatg tgcctgtcgg tgcttatctc agcggaggtc ttgattctag
     1021 tatcgtagcc actttggctg caaaaactta tcctggatct ctgaaaacgt ttacaggcgg
     1081 atttaaagat tccgcacagt atgatgaaac cctttatgct tctgtagtca gccagtctat
     1141 aggaagcgag catcatgaaa tttttccaac ttatcatgat tttttagaga attttgaatc
     1201 acttgtttac ttcatggatg aacctgttgc tggacctgga atgtttcccc agtacatggt
     1261 ttccaagctt gcttccaaac atgtaaaagt tgtgttagga ggacagggcg gagatgagat
     1321 atttggtggg tatgcccgat atgcagtagc ttatttggag cagtgtctga agggagcgat
     1381 tcttgaaacg caggaggaag agaatcatgt tgt
//
LOCUS       DAOQGJ010001313         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72063_length_1411_cov_2.476401, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001313 DAOQGJ010000000
VERSION     DAOQGJ010001313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72063_length_1411_cov_2.476401"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PK683_17445"
     CDS             complement(<1..690)
                     /locus_tag="PK683_17445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH10288.1"
                     /translation="MKNIFHSAFGEKGADLYSNAISFILNSNVDRILIFDLDGRILAC
                     NSSMIEAYGNDILGKSIYEFGSKDVSEIRRKYIEEAVSSRRAVHFEDHTENRISDAGV
                     YPIFDSSGNIILLYGITHDITELKNREKFLSLEEERYRIMISAVPDLIFRLSRDMRYL
                     DVHAADSSELYLPKEKLIGRSVFEIFSKELTSALEILVKKALDTGNEQTFEYSLELPS
                     GICWFELRIIRL"
BASE COUNT          428 a          298 c          265 g          420 t
ORIGIN      
        1 aagcctgatg attctgagtt cgaaccagca gatgccggaa ggaagttcaa gtgaatattc
       61 aaaggtctgt tcgtttcctg tatccagtgc cttctttaca agaatttcca atgcagacgt
      121 gagctctttc gagaatatct caaagacgga tctgccgatc agcttttcct tgggaagata
      181 aagttcagag gaatccgcag catgaacatc caggtatctc atgtccctgc tcagacggaa
      241 aatcagatca gggacagcag atatcatgat tctgtatctc tcttcctcaa gactcaaaaa
      301 cttttccctg ttttttaatt cagtaatgtc atgagtgatt ccgtacaaaa gaatgatatt
      361 tccggaagaa tcgaatattg gatacactcc tgcatcggag attctgttct ctgtatgatc
      421 ttcaaagtgt acagctcttc gtgaagaaac agcttcttca atatactttc ttcttatttc
      481 acttacatcc ttgcttccaa actcatagat gcttttgcct aaaatatcat ttccgtaagc
      541 ctcgatcata gaactgttgc aggcgaggat tctgccatcc agatcaaaaa taagaattct
      601 gtccacattt gaattcagta taaatgaaat tgcatttgag tacaagtcgg cacccttttc
      661 tccaaaggca gaatgaaaaa tgtttttcat tgccgatacc gccctgattt ccgcagactg
      721 aagacagata tccaatcttg cgggaaatcc atgaatgctt tccgtgaaag aaattcagaa
      781 aagcagagga aatgaagatt gaatatgaca gaattcacag ttgaccagta tttgtacaga
      841 ttcggatgaa tcaataaaaa ctttgcattg agaataagca tcctgccttc ggaaaggatt
      901 ctgcactgat tcccgatttt gtacaaggaa taacagtaaa tcctgcactt agtattatac
      961 tggttttcaa aagtctttgc gtatttatct ttcagaactc agtaaaatca agcctgccgg
     1021 gtgggaaaag cgcaattatt tatgaaaatc agtataaata cttagaaact ctgtgatata
     1081 aattttccgt tttattcggc agacagaccc atactgctgc ctccggctgc acaccgtacc
     1141 gacggcttaa gggctcgcaa atctggaatc tatggagtcg gccctacctt tgctgtctgc
     1201 gggacaattc tgctttttcg gctgtccaaa ccctgaagaa tggaaaagca ggctgacctc
     1261 gcgacattat ttttaaccct caaatcatta tttttattat gagaatatct cagataccaa
     1321 tatatataaa ggaaaacaga ataattctgc tgttcagtca gaattttccg cttgaccctg
     1381 acggaatcat tcttcatatt cagagaatga c
//
LOCUS       DAOQGJ010001314         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72094_length_1411_cov_2.019912, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001314 DAOQGJ010000000
VERSION     DAOQGJ010001314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72094_length_1411_cov_2.019912"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>1411
                     /locus_tag="PK683_17450"
     CDS             20..>1411
                     /locus_tag="PK683_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH10289.1"
                     /translation="MIFRGLYLPILIEHRIGQILFTFIAFNASVQFRLLYIAYNKEVP
                     KISWLIDATAVLTSLFALSPYLYKGYYEFSFGRSGASGPVLLLFSLVNMITLFKILYD
                     WYSFREEKKGSLQAMVMIFAVLGSILSLSAVPSTLGLPIYPLTNLQFIPSLVLAFAIL
                     KFHALPIRGETVELTNKISFASTLLVPIILLFYFGGQRIDTSRFQLVLHIVFIGSFLI
                     ISIFTFTYILLRPIAKKLDEEKEKILQAQLETETERQKSEKLLLNILPKDVASELKEK
                     GFAEPVLFESVSVMFTDFKGFTTIAENLTPNELIKDLDACFVQFDKISERFNLEKLKT
                     IGDSYMCAGGIPKKNKTHAVDCVLAALEIQDFMNMMKKLKEEKGFPYWELRLGIHSGP
                     LVAGVIGERKFAYDVWGDTVNTASRMESSGTPGRINISGNTYELVKDFFDCEYRGKVS
                     AKNKGEVEMYYVNR"
BASE COUNT          404 a          289 c          301 g          417 t
ORIGIN      
        1 cggacttctg aatatcatta tgattttcag aggactctat cttcccattc tgattgagca
       61 ccgaatcggg cagattcttt ttacgttcat tgcattcaac gcatccgttc agtttcgact
      121 cctgtacatt gcgtacaata aagaagttcc taaaatttcc tggctcattg acgcaaccgc
      181 agtgctgact tccttatttg ccctcagtcc ctacctttat aaaggttatt acgaattttc
      241 attcggaaga tccggtgcga gtgggcctgt gcttcttctg ttcagtcttg taaatatgat
      301 cactcttttt aaaatactgt atgactggta tagtttcaga gaggaaaaga aaggcagtct
      361 gcaggctatg gtcatgattt ttgcggtact cggaagtata ttaagtctat ctgctgttcc
      421 ctcgactctg gggcttccga tctatcccct tacgaatctg cagtttattc cgtctcttgt
      481 gcttgctttt gcgattctca aatttcatgc acttcctata cgaggagaaa ctgttgagct
      541 cactaacaaa atttcttttg catccactct tcttgtgccc attattctgc ttttttattt
      601 cggtggacag aggatcgata cttcccgttt tcaacttgta ctgcatatcg tatttatagg
      661 aagttttctc atcatatcca tattcacttt tacttatata cttctcagac cgattgcaaa
      721 aaaactggat gaggaaaaag aaaaaattct gcaggcacag ttagagacag aaaccgaacg
      781 tcaaaaatca gaaaagctat tactgaacat tctcccaaaa gatgtggcta gcgagctgaa
      841 agaaaaaggt tttgcagagc cggtactgtt cgaaagtgta agtgtgatgt ttactgactt
      901 caagggcttt acgactatcg cagaaaatct tacaccgaac gaactcatta aagatctgga
      961 tgcctgtttt gtgcagtttg ataaaatcag tgaaaggttt aatctggaaa aactcaagac
     1021 gatcggcgac agttatatgt gtgccggggg aattccaaaa aagaacaaaa cacatgctgt
     1081 tgactgcgtg ctcgctgctc tggagattca ggactttatg aatatgatga aaaaactcaa
     1141 agaggaaaaa ggatttcctt actgggaact gaggctcgga attcacagcg ggcctcttgt
     1201 agcgggagtc atcggagaac gtaagtttgc ttatgatgtc tggggagata ctgtaaacac
     1261 tgcttccaga atggaatcca gcggcacacc cgggagaatc aatatcagcg gcaatacata
     1321 cgaacttgta aaagattttt tcgactgcga gtaccgaggc aaggtaagcg ctaaaaacaa
     1381 aggcgaagtg gaaatgtact acgtgaaccg c
//
LOCUS       DAOQGJ010001315         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72245_length_1409_cov_3.752585, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001315 DAOQGJ010000000
VERSION     DAOQGJ010001315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72245_length_1409_cov_3.752585"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PK683_17455"
     CDS             <1..1407
                     /locus_tag="PK683_17455"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH10290.1"
                     /translation="DAQYLWQNSPFFGEVIHHWCIIFGNIVAILFTMNFLQTAKNIPR
                     VHTALKYLICLDIIIFFMPFLGHITVSIKANIALYLSTIPVFFFIGLKSYRKGVHAAR
                     FYLVAWVALMVSGIIFALSRVNIFPDNLFTNYAVQIGSTFQVLLFSFALADRINYLKK
                     EREAALLDKLSESEKVASLSRAFERFVPKQFLEFLEKESIASVQLGDNIQKTMTILFC
                     DIRSFTDLSEKMTPDDNFHFINEYLSRVGPLIRNHGGFIDKYIGDAIMALFPDHVEAA
                     VKTAVDIHRAVKEFNVQRHEKGLPRVFIGIGIHTGKLMLGTVGEENRMDTTVISDSVN
                     LASRIEGLTKKLHAPIIISSSAYSKLESSDKYPHRYLGRFRVKGKKEAVQLIEIIEDS
                     LDLYAAEKLKTKNQFEKAVNLYENQEYQEASSLFKAVMKNFPEDTASMIYILQCSKKI
                     QGIPDSEKESEESVGEEL"
BASE COUNT          432 a          262 c          302 g          413 t
ORIGIN      
        1 gatgcacagt atctttggca gaactcgccc ttttttggag aagttattca tcactggtgc
       61 atcatttttg gaaatatagt agcgatactg tttacaatga attttcttca gactgccaag
      121 aatattccca gagttcatac agctttgaaa tatcttatat gcctggatat aatcatattc
      181 tttatgcctt tcctgggaca tataactgtt tccataaaag caaacattgc actttatctt
      241 tccacaattc cggtgttctt tttcatcgga ttgaaaagtt accggaaagg agttcacgct
      301 gcaaggttct atcttgttgc atgggttgca cttatggttt ccggcattat atttgcatta
      361 agcagggtga atatttttcc ggacaattta tttaccaatt atgcagtgca gatcggttcc
      421 acatttcagg ttcttctttt ttcttttgcg ctggcagaca ggataaatta tctcaagaag
      481 gaaagagagg ctgcacttct ggacaagctg tccgaatcgg aaaaagtggc atcactcagc
      541 cgtgcatttg aaagatttgt gccgaagcag tttctggaat ttcttgaaaa ggagagcatt
      601 gcatccgttc agctgggaga caatattcag aagacgatga cgattctttt ctgtgatatc
      661 cgttccttca ccgacctttc agagaaaatg acacccgacg acaattttca ttttatcaat
      721 gagtatctga gcagggtggg acccctcatc agaaatcacg gaggattcat cgacaagtat
      781 atcggtgatg caattatggc actctttccg gatcatgtgg aagcagcagt gaagacagcg
      841 gttgacatac acagagcagt caaagaattt aatgttcaaa gacatgaaaa gggactgcct
      901 cgtgttttta tcggcatagg aattcatact ggaaagctga tgctcggaac agtgggcgaa
      961 gaaaacagga tggacactac agtaatttct gattcggtga atcttgcttc cagaatagaa
     1021 gggctgacta agaaacttca tgcccctatt ataataagct cttctgctta ttcaaaactt
     1081 gaatcttccg ataaataccc ccacaggtat cttgggcgtt tcagagtcaa gggaaaaaaa
     1141 gaagcagtcc agcttataga gattattgaa gactctcttg atttatatgc agcggagaaa
     1201 ttgaaaacaa agaatcagtt tgaaaaagca gtgaatctgt atgagaatca ggaatatcag
     1261 gaagcatcca gtctgttcaa agcggttatg aagaattttc cagaggatac agcatctatg
     1321 atttatatcc ttcaatgcag caaaaaaatt caggggattc cagattctga aaaagaatct
     1381 gaagaatctg tcggagagga attatgaaa
//
LOCUS       DAOQGJ010001316         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72270_length_1409_cov_2.630724, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001316 DAOQGJ010000000
VERSION     DAOQGJ010001316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72270_length_1409_cov_2.630724"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..277)
                     /locus_tag="PK683_17460"
     CDS             complement(113..277)
                     /locus_tag="PK683_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNH10291.1"
                     /translation="MRTNIVLDDELMKLAFQYSEFKTKKEVIQQALMEFVENRKKKNL
                     RAGKQSVFTS"
     gene            complement(508..1218)
                     /locus_tag="PK683_17465"
     CDS             complement(508..1218)
                     /locus_tag="PK683_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10292.1"
                     /translation="MTKGIFLSAVMALFLSVCNRIGVSQSCLSKCSSQNKACLAINYA
                     SNSTLTNSLSSSSSSSSSSATKTEVEPNDTFYNSNQNFNAFGEGSSTTKSTFNGTINS
                     SNDIDILPTGTTDANFKGTIFTQNNNLTSVNCELFYGGSSTLDYSNTSVPDSGFKSAG
                     KLNPSYTFPYVKDLHYAIYIICKGTANTSYSVTAEIYDPGVSPAVAVGATINSTSYAN
                     CVSSEKAYQKNCNSKGLF"
BASE COUNT          435 a          259 c          275 g          440 t
ORIGIN      
        1 ttgttgaatc agaacactgg ctcgaaaatg agaaaaagac cgaaaagaaa aaacaggtca
       61 gaaaaacttc agttccttga tcactctgga aatctggata aaatctttat cattatgaag
      121 taaatacaga ttgttttcct gctcttaagt ttttcttttt cctgttttct acaaattcca
      181 tcagtgcctg ctgtatcact tctttttttg ttttgaattc ggagtattga aatgccaatt
      241 tcatcagttc atcgtcgagg acaatatttg ttctcattta tacacctctg catacacata
      301 aatagtgtat gcagaaaatt cttacaagga aatttcattg aacgggctga aaaaaattaa
      361 aagtcttccg acggaaattt cactgcctcc cccgtatgta ttcggatgat ttgaaatcag
      421 gctgctgttc gcaaggccga tccctgtaat tcccgcatac agaatttcag gcgaccatcc
      481 gatgtgtatc cttctgaaag aagaggctca gaacagtccc ttgctgttgc agttcttctg
      541 atatgctttt tctgaagaaa cacagtttgc ataggaagtg ctgttgatag tcgcaccgac
      601 cgccactgca ggagaaacgc ctggatcata tatttcagca gtcactgaat aacttgtgtt
      661 tgctgtccct ttgcagataa tgtaaattgc ataatgcaaa tccttgacat aagggaaagt
      721 atatgaagga tttaacttac cggcactttt gaatccactg tcaggaactg aagtattgct
      781 atagtcaagg gtagaagaac ctccgtaaaa taattcacag tttaccgatg tcagattgtt
      841 attttgagta aagattgttc ccttaaaatt agcatctgtc gtacctgttg gaagtatatc
      901 aatatcatta ctagagttga ttgtgccatt aaatgtagat ttagtcgttg aactgccttc
      961 cccaaaagca ttaaaatttt ggtttgaatt ataaaatgta tcattcggct caacttctgt
     1021 ctttgttgcg gaagaggaac ttgaagaaga actgctgctg agtgaatttg tcagagtgct
     1081 gttggatgca taattgatgg caagacaggc cttgttctga ctgctgcatt tggaaagaca
     1141 gctctgactc acgccgattc ggttgcatac tgaaagaaac aaagccataa ctgctgaaag
     1201 aaaaatgcct tttgtcataa acacctcata aaatatttcc atcctcattg acaaaagcaa
     1261 gtgtcaagag tttagagttt ataaattata aattataaat tataaaaata ttaaggtatc
     1321 tgagtgcaac attaaattga aaacatatca gtaactcacc ttacgcaggt ggggcgtttt
     1381 tggaggtgag cctaccggcg gttttaagg
//
LOCUS       DAOQGJ010001317         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72295_length_1409_cov_1.830133, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001317 DAOQGJ010000000
VERSION     DAOQGJ010001317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72295_length_1409_cov_1.830133"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..724
                     /locus_tag="PK683_17470"
     CDS             65..724
                     /locus_tag="PK683_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH10293.1"
                     /translation="MSLVLKNVTKSFGNPPVDVIRSLSFEIQKGEFVSLTGKSGSGKS
                     TLLYLMSSLDFPSSGIIEINGLNISAMKEKELHRFRNRHMGFIFQFHYLLPEFTALEN
                     VLMPARKAGEHWKRRKYAEELFEKFDLKDKMNEIPGRLSGGQQQRVAIARSLIMDPDY
                     IFADEPTGALDSLNGKIVMEIFKKVNQEKKTTIILVTHDNEFANSAKRQIRLVDGEIA
                     S"
     gene            751..>1409
                     /locus_tag="PK683_17475"
     CDS             751..>1409
                     /locus_tag="PK683_17475"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH10294.1"
                     /translation="MTDDSKFDREYELTSNSPNDSRTLKSWGYVTALPSEYLLHFRNG
                     KIQEKNSGQGTTCFKFPSDTVFIIPTSLKEIIFQTNQLTKDNVDIRLKGMAVYRISDP
                     LQIYRLINFSDRSRAEQKLAGMLADICKSTIKWLVANLNLEDCIRKRKEEISEALKKE
                     LSYIVASDQGGWGIEIITINIQDIFIQDEEIFSAMQMMFKTEKLRESHFAELQMQKEL
                     E"
BASE COUNT          448 a          287 c          340 g          334 t
ORIGIN      
        1 gcattcttcc tgcaaggaag gcagggaaga tgcagcccat tgaaatcata cggggagaag
       61 tctgatgagt cttgtactga agaatgtgac gaagtctttc ggaaatcctc ctgtggatgt
      121 gatccgcagt ctgagtttcg agattcagaa gggggagttt gtttctctca caggaaagtc
      181 cggctcagga aaaagcactc ttctgtatct gatgagttcg ctcgacttcc cctcttccgg
      241 gatcattgaa atcaacggtc tgaatatttc tgcaatgaag gaaaaagagc tgcacagatt
      301 caggaacaga catatgggat tcatattcca gtttcattat cttcttcctg aattcactgc
      361 actcgaaaat gtgctgatgc ctgcgagaaa ggcaggggaa cactggaaga gaaggaagta
      421 tgctgaagaa ctctttgaga aattcgatct gaaggacaag atgaatgaaa ttccgggcag
      481 gctttccgga ggtcagcagc agagggtggc aattgcacgg tcactcatta tggaccctga
      541 ttatatattt gcagatgaac cgacaggggc tctggactct ctgaacggaa aaattgtaat
      601 ggaaattttc aaaaaagtga atcaggagaa aaagaccacg attattctgg tcactcacga
      661 caatgaattt gcaaattctg caaaaagaca gataagactg gttgacggag aaatcgcatc
      721 ctgataatgt atttgctcag ggagaacaat atgacagatg acagtaaatt cgacagagaa
      781 tatgagctca cttccaattc gcccaatgat tcgaggactt tgaaatcctg gggatatgtg
      841 accgctcttc cgagcgaata tctcctccat ttccggaacg gaaagatcca ggaaaagaac
      901 agcggtcagg gaaccacctg tttcaagttt ccgtcagaca cagtattcat cattcccaca
      961 agtctgaagg aaattatttt tcagaccaat cagctgacaa aggacaatgt ggacatccgt
     1021 ctcaagggaa tggctgtcta cagaatctct gatccgcttc agatttacag actcatcaat
     1081 ttctctgaca gaagcagggc ggaacagaaa cttgcaggaa tgcttgcaga catctgcaag
     1141 tccacgatca aatggcttgt ggccaatctg aatctcgaag actgcatccg caaaagaaaa
     1201 gaagaaatct cagaagcgct gaagaaggaa ctctcctata tcgtggccag cgatcagggg
     1261 ggctggggaa tagaaatcat tacaatcaat atacaggata tattcattca ggacgaggaa
     1321 atcttcagtg caatgcagat gatgttcaag acggaaaaac tcagagagtc tcatttcgca
     1381 gaactgcaga tgcagaagga actcgaaat
//
LOCUS       DAOQGJ010001318         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72376_length_1408_cov_2.133777, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001318 DAOQGJ010000000
VERSION     DAOQGJ010001318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72376_length_1408_cov_2.133777"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..983
                     /locus_tag="PK683_17480"
     CDS             216..983
                     /locus_tag="PK683_17480"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNH10295.1"
                     /translation="MMIKAFGATDIGKLRENNEDNFLIFRPVLKQEGDIGTDYSTENG
                     VLLLVADGMGGAAAGETASAMAVATAKACLEEYHTQNTSLELMQISILTAQKGCQMIV
                     QDRPELSGMGTVATYVYVKNNKADIAQIGDTRLYLYRNKSLEQLTEDQNLVTELVKAG
                     LITLEQAQYHPQKNAVTQAIGALDDVNPYFYTLEVQKGDILLLCSDGLTNMVSDLEIQ
                     LALDSSADLQNIPSILIDTANANGGYDNITVIVAEIQ"
     gene            997..>1408
                     /locus_tag="PK683_17485"
     CDS             997..>1408
                     /locus_tag="PK683_17485"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH10296.1"
                     /translation="MKAKLLAAGEGINIYLKYSDMPKNSDESILFVHGYPDSHSSWEK
                     QLDFFKAEFQVGAFDLRGAGKSTAPFDRSGYRISELVNDLKSVIDDMVGKDGKIHLVG
                     HDWGSIICWSFLSSPENEKRVLSYSAVTCPHPMMF"
BASE COUNT          439 a          285 c          325 g          359 t
ORIGIN      
        1 acggaaaaga tattcattgt ccgatgaatg gaagtgaaaa ttcagctccg gatgcttttg
       61 tctacagctt ttcctaaaat aactgtctct gaatctgaaa aagcagacca ctaggggata
      121 acccttccgg tgaaaatatc tgcaatcatt aagaaaaaag aaaaaaaccg gaaaatgctt
      181 gagattttgg gaacaggaaa aattctatct gcgtaatgat gataaaagct ttcggagcaa
      241 cagacatagg gaaactccgg gaaaataatg aagataattt tctgattttc cggcctgttc
      301 tgaagcagga aggggacata gggacggatt attccactga aaacggagtt cttctccttg
      361 tagccgacgg gatgggcggt gcagccgcag gtgaaacagc atccgcaatg gcagtggcca
      421 cagcaaaggc atgccttgaa gaataccata ctcagaatac atccctcgaa ctgatgcaga
      481 tctccattct cacagctcag aaaggatgcc agatgatcgt tcaggacaga cctgaacttt
      541 ccggaatggg aacagtggcc acatatgtct atgtgaaaaa caacaaggca gacattgccc
      601 agatcgggga tacaagactg tatctttacc ggaacaaatc tctggaacag ctgacagaag
      661 atcagaacct tgtgacagag cttgtcaagg caggactcat cacactcgaa caggcacagt
      721 atcatcctca gaaaaatgct gtcacacagg caatcggtgc tttggatgat gtgaatcctt
      781 atttttatac actggaagtt cagaagggtg acattcttct tctatgcagt gacgggctta
      841 caaatatggt gagtgatctt gaaattcagc tggcactgga ttcttccgca gatctgcaga
      901 atattccgtc aatactcatt gacacagcca atgccaatgg cggatatgat aatattacag
      961 taattgtagc agaaatacaa tagttcggga gataaaatga aggccaaact tcttgcagcg
     1021 ggtgaaggaa taaacatata tctgaaatat tcggatatgc caaagaattc agatgaatca
     1081 atcctttttg tacatggata tcctgattct cattcaagct gggaaaaaca gctggatttt
     1141 ttcaaggctg aattccaggt cggtgcattt gatcttcgcg gtgcaggcaa gtccacagct
     1201 ccttttgaca gaagcggata ccgaatttct gaacttgtca atgatctgaa aagcgtcatt
     1261 gatgatatgg taggcaaaga cgggaaaatt cacctcgtag gtcacgactg gggttcgatc
     1321 atctgctgga gttttctttc ctctcctgaa aatgaaaagc gagttcttag ctattcggca
     1381 gtgacctgtc cgcatcctat gatgtttt
//
LOCUS       DAOQGJ010001319         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72472_length_1407_cov_2.038462, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001319 DAOQGJ010000000
VERSION     DAOQGJ010001319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72472_length_1407_cov_2.038462"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK683_17490"
     CDS             <1..183
                     /locus_tag="PK683_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10297.1"
                     /translation="GYAQEKNAARTKEYFAKAAGLKEIYWVKVKLDSGFREMLAVQDF
                     ADFISNRGETPAKSLP"
     gene            238..>1407
                     /locus_tag="PK683_17495"
     CDS             238..>1407
                     /locus_tag="PK683_17495"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNH10298.1"
                     /translation="MPRLKSLFLFPFRLLHYIYLQIQAPFRTGNAYLLEIPSEFRESD
                     KTFFMELISGRTEKPTYLQFLSLLRRISENTDIKKLFYIAEEADFGLAEMYEIGKAFE
                     RLKNRGVRLCAYSSKGDYKTLYLLSFADERYVHPKAEFIWLLPAAESFFFHGALKKSG
                     IEVESFASGKYKSYAETFTRSGFSKEAKQNLESLLKSLNDQILETMLANTGDSDWKDR
                     SPLLNAGLLKEMKFADHMTQDDFRDNCVYEDMCAPENEDEEKEETYEILSYSSLKYRH
                     RKNRFRFIRYPKEKIAVIPLKGDITDSERDETERKEGSISSGPVLSLLREAGEMDDLK
                     YIVLEIDSGGGSAFASSMIYEQILKLKKTKKIYVYQANYSASGGYFIASASDLIFS"
BASE COUNT          434 a          267 c          316 g          390 t
ORIGIN      
        1 ggatatgcac aggagaagaa tgcagccaga acaaaggagt attttgcaaa ggctgcagga
       61 ctgaaagaaa tttactgggt caaggtaaag ctggattcag ggttcagaga aatgcttgct
      121 gttcaggact ttgcggattt tatttcaaac cggggcgaaa cacctgccaa gtctcttcca
      181 tagagggtat ataataaaaa tcttttctgt taaatgagcc tctgaaaaaa tgccagaatg
      241 ccaagattaa agagtttatt tctttttcca tttcgtcttc tgcattatat atatctgcag
      301 attcaggctc ctttcagaac agggaatgcc tatctgctgg aaataccgtc cgagttccgt
      361 gaatcggaca agactttttt catggaactc atttcaggca ggacggaaaa gcctacatac
      421 ctgcagtttc tctctttact gagacgcatt tccgagaaca ctgatatcaa aaaattattt
      481 tatattgccg aagaggctga tttcggcctt gcagaaatgt atgaaatagg caaggcattt
      541 gaacggctga aaaaccgggg tgtcagactc tgcgcctatt cttcaaaagg agattataaa
      601 actctttatc ttctgtcctt tgcagatgaa agatatgttc atccgaaggc tgaattcata
      661 tggcttctgc cagcggctga gtctttcttt tttcacggtg cactcaagaa atccgggatt
      721 gaagtggaga gttttgcatc aggaaagtat aaatcctatg cagaaacatt taccagaagc
      781 ggtttttcca aggaagcaaa gcagaatctg gagtctcttc tgaagtcact gaatgatcag
      841 attctggaaa ccatgcttgc aaatacagga gacagtgact ggaaagacag atctcctctt
      901 ctcaatgcag gtcttctgaa ggaaatgaaa tttgcagatc atatgactca ggatgatttt
      961 cgcgacaact gcgtttacga agatatgtgt gcacctgaaa atgaagatga ggaaaaagag
     1021 gaaacttatg aaattctttc gtacagttca ctgaaataca ggcacagaaa aaaccggttc
     1081 agattcatcc gctatccaaa ggaaaaaata gcagtgattc ctctgaaggg agatattaca
     1141 gattcggaaa gggatgaaac ggaaagaaag gaaggcagta tttcttcagg tcctgtcctg
     1201 agtctccttc gggaagcggg agagatggat gatctgaaat acattgttct cgaaattgat
     1261 tcaggcggag gttctgcatt cgcatcttcc atgatttatg agcagattct caagcttaaa
     1321 aaaactaaaa agatttatgt ttatcaggcc aactattccg catccggggg gtatttcatc
     1381 gcatctgcat ccgatctgat tttctcc
//
LOCUS       DAOQGJ010001320         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72548_length_1406_cov_2.790526, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001320 DAOQGJ010000000
VERSION     DAOQGJ010001320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72548_length_1406_cov_2.790526"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1337)
                     /gene="glmM"
                     /locus_tag="PK683_17500"
     CDS             complement(3..1337)
                     /gene="glmM"
                     /locus_tag="PK683_17500"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNH10299.1"
                     /translation="MISVSGIRGRIPEGLFLENSLNFINSFIETLNPKSVIIGRDSRP
                     SGIFLENFVTGILLSKGIRAVNLGLVPTPTVKSVVKETNSSGGIMISASHNPIDWNAF
                     KLIGKNGFFFGPSQMEIFKEHSVRMDFRPPLFNPKAKVLSDPAYIRLHIESVLKRVNV
                     PKIRKKKFRVFLDAVNGGGSKVVPMLLEELGCKVYPLYCEGEGGFPREPEPTPKALEK
                     TAKQMRKTDAQIGFALDPDADRLVLLTPERGCISEEYTLPLSLLSVLPGNRNTVVTNL
                     SSSFITEKVASEFGKIVLRSKVGEANVVAEMLNARSMFGGEGNGGVIDPEISSFGRDS
                     LAGIAHILNVLAEKNQSIDSLLDEMPEIHMEKTSYSVKDRNLSEILLKFKTSFPSKKI
                     DERDGLWMDLGGKWIHLRASNTEPIIRLIGEAETERDLKEMMKRSSQVIAGE"
BASE COUNT          359 a          310 c          292 g          445 t
ORIGIN      
        1 ttttactcac ctgcaatcac ctgggaggat cttttcatca tttctttcag atcacgttct
       61 gtttccgctt caccgatcag ccgaatgatt ggttccgtat tggatgctct caaatgaatc
      121 cattttccgc ccagatccat ccagagtccg tctctttcat caattttttt ggatggaaac
      181 gaggttttga attttaaaag tatttcactt agatttctgt ctttgactga gtaagaagtc
      241 ttttccatat gaatttcagg catttcatcg aggagcgaat cgatcgactg atttttttca
      301 gccagtacat tcagtatgtg tgcgatgcct gccagcgagt ctcttccaaa ggaagaaatt
      361 tccggatcaa tgacccctcc gtttccttct ccgccgaaca tagatctggc attcagcatt
      421 tctgcaacta cgtttgcctc accgaccttg gatctgagca caatttttcc gaattcggaa
      481 gccacttttt ccgtaatgaa actgctcgaa agatttgtaa caactgtgtt cctgtttccc
      541 ggaagaacag aaagcagact taacggaaga gtatattctt cagagatgca ccctctttca
      601 ggagtcagaa ggactagtct gtctgcatca gggtccaatg caaatccgat ctgagcatct
      661 gtctttctca tctgttttgc tgttttttcc aaagccttgg gagtgggttc cggttctcta
      721 ggaaaccctc cctccccttc acagtacaga ggataaacct tgcatcccag ttcttcgaga
      781 agcataggaa cgactttgct ccctcctcca ttgactgcat ctaaaaatac tctgaacttt
      841 ttcttcctga ttttaggaac attgactctt tttaaaactg attcgatatg gagacggatg
      901 tatgccggat cagaaagaac ctttgccttt ggattaaaaa gcggaggtcg gaaatccatt
      961 cggactgaat gctccttgaa aatttccatc tgtgaaggac cgaaaaagaa tccatttttt
     1021 cctatcagct tgaatgcatt ccagtcgatc ggattatgag atgcactgat cataattccg
     1081 ccggaactgt ttgtctcttt gactactgat ttgaccgtag gtgtagggac aagacctaag
     1141 ttgactgcac ggattccttt ggatagaagt attccggtca cgaaattttc taaaaatata
     1201 ccagagggtc tggaatcgcg tccaatgatg actgattttg gattcagtgt ttcaatgaat
     1261 gagttgatga aattgaggga attttccaga aataaacctt ctggaatcct tcccctgatt
     1321 cctgatacgg aaatcataag gttgggattt gaatttagct gagccatgtc ggtgtctagg
     1381 ttttatcgtg ggcgatgaca ggattg
//
LOCUS       DAOQGJ010001321         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72744_length_1404_cov_2.825797, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001321 DAOQGJ010000000
VERSION     DAOQGJ010001321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72744_length_1404_cov_2.825797"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PK683_17505"
     CDS             <1..612
                     /locus_tag="PK683_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10300.1"
                     /translation="EERYLGTNPLNKDSDMDGLLDGTEMELGLNPLKAQSKEEVRKII
                     RDKGPFIDGSPADWDLIENKKTFISAPNRSLKGKYDLIEFHYIIKNNIFYGMMKTREL
                     PAFDSASDKDTYFFINDVTKPKFRDGFGCWYSPTARTGWEYGRSSGESAVPEGRLGEV
                     FEWKIPLSDSDPFPLEFVPIVRNGKVKENLGYWNEYKPIIIEK"
     gene            complement(897..>1404)
                     /locus_tag="PK683_17510"
     CDS             complement(897..>1404)
                     /locus_tag="PK683_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10301.1"
                     /translation="GIFWMSLEDFFGKMGTIKVTAIGFDHLPDTETVSSVWGLDTNYF
                     EFKEDLQEVTDICFRQIDEPDSDEKIFESLPQYKLELNERTDFIGIVDKSYSTFAYIF
                     PNTSGQRIYDLSLITGTEYAVSVNWVFSAFELDAGVYTVVFFTRDEAGIETVFHLWTK
                     EKLDLAEI"
BASE COUNT          464 a          282 c          272 g          386 t
ORIGIN      
        1 gaagagcgtt atttgggaac aaaccctctg aacaaggact cagatatgga cggacttctg
       61 gacggaacag aaatggaact tggactgaat ccattaaagg cacagtccaa ggaggaagtg
      121 agaaaaatca tcagagacaa gggacccttc attgacggct cacccgctga ctgggatctc
      181 attgaaaaca aaaagacttt catttctgca ccgaacagat cactgaaagg aaaatacgat
      241 ctgatagaat ttcattatat aataaagaat aatatttttt atggaatgat gaaaacaaga
      301 gaactgcctg ccttcgactc agcatcagac aaggatacat attttttcat taatgatgtc
      361 accaagccta aattcagaga tggattcggt tgctggtatt caccgactgc aagaactgga
      421 tgggagtatg gacgaagttc cggagaatct gcagtccctg aaggaagact tggagaagtg
      481 ttcgagtgga aaattcctct ctcagattcg gatccgtttc cgctcgaatt tgtcccgatc
      541 gtgcgaaacg gcaaagtgaa ggaaaacctc ggctactgga atgaatacaa accgatcatt
      601 atagagaaat gaggtaacag caccagcagt cacgggacaa acgaactgct gatgtaaaaa
      661 aaatataaat aactatgctg tatatattag agttgttgaa aaattagttt caacaactct
      721 aatttcaatt tttctaaaaa acttgcagct cgctaaagcg agctgagtta atcctaccgg
      781 aggtttcaag ggactatcgt cccttgaaaa tccctattta gcttctgatt gaagataatt
      841 tcttgatttt ttcaacaact ctattttatt cagtgagctg catccagggt tctgacttaa
      901 atctctgcaa gatccagctt ttccttcgtc cataaatgga aaacggtctc tataccagcc
      961 tcgtctctcg taaaaaaaac aactgtataa acaccggcat ccagttcaaa ggcagaaaaa
     1021 acccaattga cagaaacagc atattctgta ccggttatta aactcaaatc atatattctt
     1081 tgcccggaag tgtttggaaa aatatatgca aaagtactgt aacttttatc cacaattcca
     1141 ataaaatctg tcctttcgtt aagttccaat ttatattgag gaagagattc aaatattttt
     1201 tcatcagaat caggttcatc gatttgcctg aaacagatgt ctgtgacttc ctggaggtcc
     1261 tctttaaact cgaagtaatt agtatccagt ccccatacac tggatactgt ttcagtgtcg
     1321 ggaaggtggt caaatccgat ggctgtaact ttaattgtcc ccatttttcc gaagaaatcc
     1381 tcaaggctca tccaaaaaat acca
//
LOCUS       DAOQGJ010001322         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72816_length_1403_cov_3.373145, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001322 DAOQGJ010000000
VERSION     DAOQGJ010001322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72816_length_1403_cov_3.373145"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1403)
                     /locus_tag="PK683_17515"
     CDS             complement(<1..>1403)
                     /locus_tag="PK683_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNH10302.1"
                     /translation="REGLDILEFFISTHGARKGLSDTALKTADAGYLTRRLVDISQDV
                     IITEPDCGTETCINLSVVKEGENVIVSLADRVFGRYTAEDIIDPVTEKVVYPKNTLIV
                     RSVGQKIENLGYTKVKVRSPLTCESKHGICTMCYGMDMARLVPVEIGEAVGTIAAQSI
                     GQPGTQLTMRTFHIGGAASAKIKEKEHKVSYQSYVKSVSSSKLIKNGLGQSVFSKPGN
                     IVVQNLIQTLSTAELVNVRIQDGQKVEKGEVIATTAKGEMVHATMGGTVTIHGNDFRI
                     LGDDVVIPVKIGTILHVKEGDVIPPGKPLAEFDPHNDLVITDAEGTVSLVDLEVGKNL
                     RRDEDPKTGNVTLKVIENRRDKLSPRILITKADGSVDEYPAPIDAQISLKNGDTVKIG
                     DAIFKIPSIAEKTRDITGGLPRVDELFEARRPKDAATLAEISGRVEDRGEIAKEKRIL
                     YINPDSSEMEKVKVAIP"
BASE COUNT          340 a          352 c          268 g          443 t
ORIGIN      
        1 gaggaatcgc aacttttacc ttttccattt cggaactgtc aggattgata tacagaattc
       61 ttttctcttt tgcaatttct cccctgtcct ccactcttcc gctgatctca gccagagtag
      121 cagcatcctt agggcgtctt gcttcaaaaa gttcatctac acgaggaaga cctccggtaa
      181 tatcccttgt cttttctgca atcgaaggaa tcttgaagat cgcatcccct atcttgacag
      241 tatctccatt tttcagagag atctgtgcat caataggagc aggatactca tctacagaac
      301 cgtcagcctt tgtgatgaga attctaggac tgagtttatc ccttctgttt tcaatgactt
      361 tcagtgtaac gtttcctgtc ttagggtctt catctcttct gaggttcttc cctacttcca
      421 ggtcaaccag tgaaacagtt ccttctgcat ctgtgatcac aagatcatta tgaggatcaa
      481 attctgccag gggctttcct ggaggaatca cgtccccttc tttcacatgg agaattgttc
      541 caatttttac agggatgaca acatcatcac ccagtatccg gaaatcatta ccgtgtattg
      601 taactgttcc acccatcgtt gcatggacca tttccccttt tgcagtggtt gcaatcactt
      661 cacccttttc caccttctgc ccgtcctgaa ttctgacgtt gacaagttcg gcagtgctga
      721 gtgtctgaat aaggttctgc acgacaatgt ttccaggttt actgaatacg gactgaccca
      781 gtccattctt gatcagtttg ctgctgctca ctgattttac ataggactga taagaaactt
      841 tatgttcttt ttccttaatt tttgcggacg cagcaccccc gatgtggaat gttctcatcg
      901 tcagctgtgt tccaggctga ccgatggact gtgcagcaat tgttcctact gcctcaccga
      961 tttccacagg aacgagtcta gccatatcca ttccgtaaca cattgtacag attccatgtt
     1021 tggattcaca ggtgagagga gaacgaacct tgactttagt gtaacctaaa ttttcaatct
     1081 tctgaccgac tgatctgaca atcagtgtat tcttaggata aacgactttt tcagtcactg
     1141 ggtcaataat atcctctgca gtgtatcttc cgaatactct gtctgcaaga gaaacaatta
     1201 cattttcccc ttccttcact acactcagat taatgcaagt ttctgtgccg cagtccggtt
     1261 cagtgatgat cacatcctga gaaatatcca caagtcttct tgtaagataa ccggcatccg
     1321 ctgttttgag agcagtatct gaaagccctt ttctcgcacc gtgagtggaa atgaagaact
     1381 ccagaatgtc cagaccttca cgg
//
LOCUS       DAOQGJ010001323         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72904_length_1402_cov_3.673348, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001323 DAOQGJ010000000
VERSION     DAOQGJ010001323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72904_length_1402_cov_3.673348"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1306)
                     /locus_tag="PK683_17520"
     CDS             complement(92..1306)
                     /locus_tag="PK683_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10303.1"
                     /translation="MLRAVKICVMHGMQIEKKTKAAIKNNRFEMEKASPSRMLEEYNK
                     IIRTWDSANILKGLAENFLLEVLFKDALSITMKNPRWREEFLNLSIGKRLQIADRKLK
                     EREDIPNVVFYALLFSEVVEEALSKEKGNKIPIIKQTLDSITRKMELPKREKDILVKI
                     YASQKRFLRKEEDKNNHNESFKTKDFFYESFIYFKINAEANNDEEAIQTALYWEISSR
                     MKARQDEKKAGDEKKKTHHQNNFNNGKNNKNFPKDKNRPHHHQKDNKSLEKKEESVKE
                     ETPVQKEETESKQISENSGESKHIHKPRNKHERKKFPYKKPEENSETAAVQENEVSAD
                     TAPVQTEGQSATEEKTEEGAPKAKKFHKKNRYKRFRKNKFNKNPNNQNNGGQGGGEKP
                     QSSQSSEGGGTQ"
BASE COUNT          282 a          304 c          290 g          526 t
ORIGIN      
        1 ctagagctga atgtcaagat gcggaaatca ttcggtgacc ggcgtgaaaa ctatatgtcc
       61 cgattaaact cggcacaggt cgggactttt tttactgagt cccgccgcct tcagaggatt
      121 ggctggactg aggcttttcg ccgccgccct gaccgccatt gttctgattg tttggattct
      181 tgttgaactt gttctttctg aatctcttat accggttctt tttatgaaac tttttcgctt
      241 taggagcgcc ttcttctgtt ttttcttcgg tggcagactg tccttccgtc tgaacaggag
      301 cagtgtcagc agagacttcg ttttcctgaa cagcagcagt ttctgagttt tcttccggtt
      361 ttttataagg gaattttttt ctctcgtgtt tgtttctagg cttgtgaata tgcttggatt
      421 ccccggaatt ctcggagatt tgtttggatt cagtttcctc tttctgaaca ggggtttctt
      481 cttttacaga ttcctctttc ttttcgagag atttattatc cttctggtga tggtgaggac
      541 ggttcttatc ctttggaaag ttcttgttgt tctttccatt gttgaaattg ttctggtgat
      601 gagtcttctt tttctcatcc ccagctttct tttcgtcctg tcttgccttc atcctgctgg
      661 aaatttccca gtacagtgca gtctggattg cttcttcgtc attgtttgct tcggcgttga
      721 tcttgaaata gataaatgat tcatagaaga aatcctttgt tttgaaggat tcattatgat
      781 tgtttttatc ctcttccttg cggagaaatc tcttctggct tgcataaatt tttaccagta
      841 tgtccttctc tcttttggga agttccattt ttctagtgat ggaatccagt gtctgcttaa
      901 tgatcgggat cttgtttccc ttttccttgg aaagggcttc ctctacaacc tcagagaaca
      961 gaagcgcgta gaatactaca ttggggatat cttctctctc tttcagtttt ctgtctgcaa
     1021 tctgaagtct ttttccgatg gacaggttca aaaattcttc tctccagcgc ggattcttca
     1081 ttgtgatgga aagagcatct ttgaaaagaa cttccagcag aaagttttcc gcaagacctt
     1141 tcagtatatt tgcggaatcc catgtacgga tgatcttgtt gtattcttcc agcattctgg
     1201 aaggagatgc tttctccatc tcgaatctgt tgttcttgat cgcggctttt gtcttctttt
     1261 caatctgcat cccatgcatc acgcagatct tcactgcacg aagcattctg actggatctt
     1321 ctttgaaaga aatctccgga tcgcctacta caattcaaaa atttagctgg cctatgccca
     1381 gagacagtta tgcagatgta ct
//
LOCUS       DAOQGJ010001324         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_72972_length_1402_cov_1.671863, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001324 DAOQGJ010000000
VERSION     DAOQGJ010001324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72972_length_1402_cov_1.671863"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1239)
                     /locus_tag="PK683_17525"
     CDS             complement(157..1239)
                     /locus_tag="PK683_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10304.1"
                     /translation="MYFSASAQENSPASPTKDTVPAETPKEVLTVTQYTANDINFRGS
                     NIFGDKLSRRNLQPYSSFAQSWFLSTNVKFDLPVNGLKLYMTSHNPLENRSDRDTDFR
                     FQSSPGGTDETSRLNSFLGTGNLTFDPNAVKFRKEHNGLRDVFVAQIYYEWTNFAGGF
                     KTGFFILSNQNYPTKFNMYEYVFGWEIPILKVIHPEWTTYYRLSSELNGLNNGNIHSR
                     MSFKHEFFKESILKFTPALEAGYQAPNNSTDRRVGVSDVTLKLQFSLKSLFLNLIDAY
                     RPNMYMYDNDRYFSKTGVLSDINPNDGRTVDPSKTAGAGNKTAIDGIESLPVHEVIKS
                     AMVRDYQEQKIPKHIFIVQIGYNKKF"
BASE COUNT          376 a          283 c          287 g          456 t
ORIGIN      
        1 ggctcagcga cttggacact gccgaaaatc ccgcaagtgc gggatccgct gtttatactg
       61 attcattaca ggctgataaa ctgtgctgga tttccctgat gctttttgaa aattacagcg
      121 tccggacagc ataccatatt tgcataaggt gaattattaa aattttttat tatatccaat
      181 ctgtacaata aatatatgct tgggtatctt ctgttcctga tagtctctga ccattgcaga
      241 ctttatgact tcatgcaccg gaaggctttc tattccgtct attgcagtct tattgcccgc
      301 accggctgtt ttggagggat ccactgttct gccgtcattc ggattgatgt ctgatagaac
      361 gcctgtcttc gaaaaatatc tgtcattatc atacatatac atattcggtc tgtaagcatc
      421 gatcagattc agaaacaggc ttttcagtga aaactgaagt ttcagtgtga catcactcac
      481 tcccactctt ctgtccgtgg aattgttcgg agcctggtat cctgcttcga gtgcaggagt
      541 aaatttcaga attgattcct tgaagaattc atgcttgaag ctcatcctgc tgtgaatatt
      601 gccgttgttc agtccgttca gttcagaaga gagtctgtaa taggtcgtcc attcgggatg
      661 aatgactttc agaataggaa tttcccatcc gaatacatat tcatacatat tgaatttagt
      721 cggataattc tgattgctca gtataaaaaa tccggtttta aatccgcctg caaagtttgt
      781 ccattcatag tagatctgtg caacaaatac atctctcaga ccattgtgct cttttctgaa
      841 ttttactgca ttcggatcaa atgtcaggtt ccctgtgcct aaaaaagaat tcagtctgga
      901 tgtttcatct gtcccgccgg gagaagactg aaatctgaaa tcggtatccc tgtctgatcg
      961 gttttcgaga ggattgtggg atgtcatata cagtttcagt ccatttacgg ggagatcaaa
     1021 tttcacgttt gtagacagaa accaggactg tgcaaaagag ctgtacggct gaagatttct
     1081 tctgctgagc ttgtctccga atatgtttga gcccctgaag ttgatgtcat ttgcagtgta
     1141 ctgagtgact gtcagaactt ccttcggggt ttctgcagga acagtatcct ttgtcggact
     1201 ggcaggagaa ttttcctgtg ctgaagcgga aaaatacacc gaagataaaa acagtataac
     1261 tttaaaaatt aggaacattc atttcaaaaa tgaatgtttc cccataaagt caatgattat
     1321 ctttttatta tatcatgtaa atactattga atctttttta caaattcgga tttgagctgg
     1381 attgctccga tcccatccag tt
//
LOCUS       DAOQGJ010001325         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_73111_length_1400_cov_3.043866, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001325 DAOQGJ010000000
VERSION     DAOQGJ010001325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73111_length_1400_cov_3.043866"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..988
                     /locus_tag="PK683_17530"
     CDS             113..988
                     /locus_tag="PK683_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10305.1"
                     /translation="MRGFTDFIRISLFLCLILFSGCTGQLKLISGMYIEDYYHPDDII
                     GIYNNCKESGHCRIGIKIKFRNRQGMTRIGLSELQTWNSKNVEKYYWLESPSESGAFE
                     QGLVLMNTVQRRLKKLEVPLRKNGVILDFSGIFQEKQQVIIPFEKPVNSELDSVTVSE
                     KKDFLRFRYADRSDVYYKNIYHNKASGCFEYKIQHEKPDFYENTGFKVRKEILDFRKS
                     LLLNLNDRLILVQAENLDFSSAGRLNVPHLTSNKKWIHNGLGDYDRHRENSGKIDLSF
                     LYALELLFRISQILK"
BASE COUNT          441 a          250 c          292 g          417 t
ORIGIN      
        1 aaaatggagg ataaggatta tgaaaacgaa atcaggaaca tccattattc ccttgcatat
       61 ctgaattacg aattctttct ttcactccat cctaaaacaa ggaaaacgat tgttgagagg
      121 ctttactgat ttcatccgta tctcactctt cctgtgtctg attcttttca gcggatgcac
      181 aggacagctg aaactgatta gcggtatgta tatcgaagat tattatcatc ctgatgatat
      241 tattggaatt tataataact gtaaagaatc cggacattgc aggatcggaa tcaaaatcaa
      301 attcagaaac cgccagggaa tgaccagaat cggtctttcg gaacttcaga cctggaattc
      361 caagaacgtc gagaaatatt actggctgga aagcccctct gaatcaggtg catttgagca
      421 aggtcttgtc ctgatgaata ctgtccagag aagactgaaa aaacttgaag ttcctttaag
      481 gaaaaacgga gtaattctcg atttttcagg aatatttcag gaaaagcagc aggtgataat
      541 tccgttcgag aaaccggtga actcagaact cgattctgtg actgtatcag aaaaaaagga
      601 ctttctgcgg ttcagatatg cagacaggag tgatgtttat tataaaaata tatatcataa
      661 taaggcatca ggctgttttg agtataaaat tcagcatgaa aaacctgatt tttatgaaaa
      721 tacgggattc aaagtcagaa aagaaatcct ggatttcaga aaatcactcc ttctgaatct
      781 gaatgacagg ctgattctgg ttcaggctga gaacctggat ttttcttcag ccggcagact
      841 gaatgttccg catctgacct cgaataaaaa atggattcat aacggtctgg gtgattatga
      901 cagacacagg gaaaattccg ggaagattga tctctcgttt ctctatgctc tggaactcct
      961 tttcagaatt tcccagatat tgaaatgatt atataaaaat attgcatgct tatttcagca
     1021 ggttcagaga aaacagattc catgtctatg taattgcaga tcaggatact tcagtttcgg
     1081 aggactggaa tgatatttca tacgaaagga aatggatcgg aattttcagt cagtaatctg
     1141 atgcctgcac tggccgattc agcatatgag aagtacttcg gtgcgaaatc attcttatcc
     1201 atatcggccg gtacaaagat ttctgaggtc aggaatgaca atgttaccta cattcctatt
     1261 ttaggagttt cggttacttt ataaaacttt ttagtatcct gccattcgac agttcctgtt
     1321 tcgagacgga taagctgcat tgtgatggaa agatactgaa gttttttatt gtctgcaaag
     1381 cggacatttt cgctgatgtc
//
LOCUS       DAOQGJ010001326         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_73151_length_1400_cov_2.243866, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001326 DAOQGJ010000000
VERSION     DAOQGJ010001326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73151_length_1400_cov_2.243866"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK683_17535"
     CDS             complement(<1..237)
                     /locus_tag="PK683_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10306.1"
                     /translation="MIKGVLIDFDGVISRSSVQNTIIFTHEYINRVQPIPYKFIENYI
                     KLILSFPVESSVALLFDSLGLSSLIKDFYTKFNDL"
     gene            complement(234..926)
                     /locus_tag="PK683_17540"
     CDS             complement(234..926)
                     /locus_tag="PK683_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10307.1"
                     /translation="MRGLTSIRLVALDIDGVALHDTFSPIIYNLAKRWGKEYTRDLER
                     NVFSQPRMKAARYMIDSFGLDLTETELLDLYFQERKRYLETESGGQVEGLEDFLHLCK
                     EMNLPVICYGGLDKTHFDEKMGNLAHFFEGEKYISTDSFRPGIKEIVTEYYRFHYSEV
                     IFIDDVNKVAEEAKKFDVPFIGIPSSFEYGFQKFDMKQTGVKFILNSIRDLTEDVLRN
                     VDEENRLGRIWK"
     gene            complement(927..>1400)
                     /locus_tag="PK683_17545"
     CDS             complement(927..>1400)
                     /locus_tag="PK683_17545"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNH10308.1"
                     /translation="TVTFYQKNKEVASIEYETSIVNKALFSRLRSDKDQYDQLPRFRP
                     LPGFENNMSLRDGTNRYLCSIIDVSLSGFLLKFDSDDFLFKTKQPEDLELEFFMFFPK
                     VGFIHGQCKMVWSLADADDKYIAGFHIVKIDKLDMNNLKESIKFYFKVREDREIV"
BASE COUNT          459 a          296 c          228 g          417 t
ORIGIN      
        1 caaatcatta aattttgtat aaaaatcctt tattaaggaa cttaatccta aagaatcaaa
       61 caaaagagca actgaagatt ccacaggaaa agacaggata agcttgatat aattttctat
      121 gaatttatat ggaatgggtt gaaccctatt tatatattca tgcgtaaata tgattgtatt
      181 ctgtacggaa gatctggaaa tgacaccgtc aaaatcaatc agaactcctt ttatcatttc
      241 catatcctgc caagacggtt ctcctcatcc acatttctca gaacatcttc agtaagatca
      301 cgaatagagt tcaaaataaa cttaacacca gtctgtttca tatcaaattt ctgaaaacca
      361 tattcaaatg atgacggaat tcctataaaa ggaacatcaa atttctttgc ctcctcggca
      421 actttgttta catcatctat aaatattact tcagaataat gaaaccgata atattccgtt
      481 acaatctctt ttatgcctgg cctgaaactg tcagtgctaa tatatttctc accttcgaaa
      541 aaatgagcca gattgcccat tttttcatcg aagtgagttt tatccagacc accataacat
      601 ataacaggca agttcatctc tttacaaaga tgcagaaaat cttccagtcc ctcaacttgt
      661 ccgcctgatt cagtttcaag atatcttttt ctctcttgaa aataaagatc aagaagttcc
      721 gtttctgtaa ggtcaagccc aaaggaatct atcatataac gagctgcttt catacgaggc
      781 tgagaaaaaa cattcctttc cagatctcga gtatattctt ttccccaacg ctttgcaaga
      841 ttgtaaataa ttggactgaa agtatcatgg agagctaccc catcaatatc gagtgcaacg
      901 agccttatag aagtaaggcc tctcatttac acaatctccc tgtcctcgcg gaccttgaaa
      961 tagaacttaa tggattcctt gagattattc atatccaatt tatcgatttt cacaatatga
     1021 aaaccggcga tatatttgtc atcggcatct gcaagactcc agaccatttt acactgaccg
     1081 tgaataaaac cgaccttcgg aaaaaacatg aagaattcca gttcaagatc ttccggctgc
     1141 ttggttttga acaggaagtc gtctgaatca aatttcagta aaaaaccgct gagagacaca
     1201 tctatgatac tgcagagata acggttagta ccgtcacgca atgacatatt gttttcaaat
     1261 cccggcagag gacggaaacg gggaagctga tcgtactgat ccttgtctga ccgcagacgg
     1321 ctgaacagtg ccttgttgac aatactggtt tcatattcaa tggaagcaac ttccttgttc
     1381 ttctgataaa aagtgactgt
//
LOCUS       DAOQGJ010001327         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_73391_length_1397_cov_3.091654, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001327 DAOQGJ010000000
VERSION     DAOQGJ010001327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73391_length_1397_cov_3.091654"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1294
                     /locus_tag="PK683_17550"
     CDS             221..1294
                     /locus_tag="PK683_17550"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10309.1"
                     /translation="MKSFSELKNAGHEKLPQTLIYVSLDSYEFDLITDFYKEKFRKSG
                     EVFETVIFVAENGDMEKLFSEVFSFSMFASSKLIIIWSGEDFFKTILSAGKKDLFENF
                     RKNVPNIPDTYYILVHYDAKDIPAKMNQLFSGNFSVIRNKQLYPEERKRVLEEILKSE
                     KITLDADALDEFMQRIPSNSGAYLRNILKVRALLEPKKHFTKEDMENTLFAVSEINPF
                     QLADSFFSVNQAEFFKEFGKLQKTQEDRQGKFLSLLSIFLNRADEIRKAKTLLKAFRN
                     DSDEKEFFRLLGMEHFSEGRKRFVKGRLRRESTVFSDQVLEKIYDMLYEINFKMKTGL
                     AQDLELGLFISRSEEIFLMLKRG"
BASE COUNT          451 a          247 c          299 g          400 t
ORIGIN      
        1 gtctagccaa aagacaggat ccgcaatctc gcgccgatcg gctgaagctg gtgtatttac
       61 cggaggcttc agagaacttg ggaaccttgt ccgccaacta cgtactcaaa aaactaaaca
      121 atctgttact gacttgactc aaaacacatc agtaaactat atctactttg tacacatgaa
      181 aaaagaaagt caagcaaaaa aaaatttttc tccaaaagaa ttgaaaagtt tttctgagct
      241 gaaaaacgca ggtcacgaaa aactgcccca gactttaatt tatgtttctt tggattccta
      301 tgaatttgat ctgattactg atttttacaa ggaaaaattc agaaaatccg gtgaagtgtt
      361 tgagaccgtg attttcgttg cagagaacgg tgatatggag aagctgtttt ccgaagtctt
      421 cagtttcagt atgtttgctt cttcaaaact catcattata tggtcagggg aagatttttt
      481 caagacgatt ctttcagcag gcaagaaaga tctgttcgaa aatttccgca aaaatgttcc
      541 gaacataccg gatacatatt atatacttgt tcattatgat gcaaaagaca ttcctgcaaa
      601 gatgaatcag cttttttccg gaaatttttc tgtgatacgg aataagcagc tgtatcccga
      661 agaacgcaaa cgtgttctgg aggaaattct caagtcggaa aaaatcacac tggatgcgga
      721 tgcactggat gaatttatgc agaggattcc ttccaactca ggagcgtatc tcagaaatat
      781 tctgaaggta agggctcttc ttgaacctaa aaaacatttt actaaagagg atatggagaa
      841 tactcttttt gcagtcagtg agatcaatcc ttttcagctt gcagacagtt tcttttccgt
      901 aaatcaggca gagtttttca aggagtttgg aaagctgcag aaaactcagg aggacagaca
      961 gggaaaattt ctgagccttc tcagcatatt tctgaacagg gcggatgaaa tacgcaaggc
     1021 caagactctt ttaaaggctt tccgaaatga ttctgatgaa aaggaatttt tccgtcttct
     1081 ggggatggag catttttccg agggaagaaa gaggtttgta aagggaagac tgaggagaga
     1141 gtccactgtt ttttcggatc aggttctgga aaaaatttac gatatgcttt atgaaatcaa
     1201 ttttaagatg aagacagggc tggctcagga tctggagctt ggactcttca tatccagatc
     1261 ggaagaaatc tttctaatgc tgaaaagggg ataaatgaac attataaaat attgcaattc
     1321 ggtcaatgtc tccttagttg tgaaattcag cgcaggatct ctgaataaaa tcaggcctga
     1381 tatgtttcaa acagaca
//
LOCUS       DAOQGJ010001328         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_73590_length_1395_cov_2.935075, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001328 DAOQGJ010000000
VERSION     DAOQGJ010001328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73590_length_1395_cov_2.935075"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK683_17555"
     CDS             complement(<1..267)
                     /locus_tag="PK683_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HNH10310.1"
                     /translation="MGREKLHCSFCGKEQDAVKRLVAGPGVYICDECIALCMEIIGEE
                     PVLAEKTSAIGDIPKPVDIKKILDQYVIGQHQAKKALSVAVYNHY"
     gene            complement(291..875)
                     /locus_tag="PK683_17560"
     CDS             complement(291..875)
                     /locus_tag="PK683_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNH10311.1"
                     /translation="MYVPHVTELTSRGERSNDLFSRLLQDRIVFIGTGIDNSYANVII
                     AQLLFLEADNPEKDIYVYINCPGGYIQAGLAVYDTMQFIRPDIRTICIGQAVSIASLI
                     LAAGTKGKRSSLPNSRIMMHQPIGDATGQASDIEIQANEILRVKQIVNSIYEKHTGKP
                     AEMIERDIERDLYMTAEEAKNYGIIDNVFSERKS"
     gene            complement(880..>1395)
                     /locus_tag="PK683_17565"
     CDS             complement(880..>1395)
                     /locus_tag="PK683_17565"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10312.1"
                     /translation="MEYAERQLQSYYRILAFNAVRKESEIQIPETMIDDEMNSIFHDA
                     IHENNLEHMSMEEFAKKFEISLDQMKETYRKRAILNLENSLVHIEIAKAENLTVSETE
                     LKAEMEYELQRYQQTERKKIDPIKLAQNLHYGMLLNKARKFIVDNAERSAVENITLSK
                     MEKLFSEPLED"
BASE COUNT          348 a          320 c          250 g          477 t
ORIGIN      
        1 gtagtgatta taaacagcta cagaaagagc tttttttgcc tgatgctgtc cgattacata
       61 ctgatccaga attttcttga tatcgactgg cttaggtatg tcaccgatag cagaagtctt
      121 ctccgccaga acaggctcct ctccaatgat ttccatacag agagcaatac attcatcaca
      181 gatatatacc ccaggacctg caacaagccg tttaaccgca tcctgttcct tgccgcagaa
      241 tgaacaatgg agtttttctc tgcccataga agtttttttt cctgccacgc tcagcttttc
      301 ctctcagaga aaacattatc tattattcca tagttctttg cttcctctgc cgtcatatac
      361 agatctcttt caatatccct ttcaatcatc tctgcaggct tgcctgtatg tttttcataa
      421 atggaattta caatctgctt tactctcagg atttcgtttg cctgaatttc tatgtcactt
      481 gcctgacctg ttgcatcacc gataggctga tgcatcataa ttctggaatt aggaagcgag
      541 gatctttttc cctttgttcc tgctgcgagg atcagtgagg cgatggaaac agcctgtcca
      601 atgcatattg ttctgatgtc gggtctgatg aactgcattg tatcataaac ggcaaggcct
      661 gcctgaatgt atccaccagg acagttgata taaacatata tatccttttc aggattgtcc
      721 gcttccagaa acagaagctg tgcaataatg acattcgcat aggaattgtc gattcctgtc
      781 cctataaata caattctgtc ctgcagaaga cgagaaaaaa gatcattgga tctctctcct
      841 ctgcttgtga gttctgtaac gtgcggaaca tacatatttt caatcctcca atggttcgct
      901 gaagagtttt tccatctttg acagagtaat attctcgact gccgatcttt cagcattgtc
      961 tacaataaac tttctggctt tatttaacaa cataccatag tgcaggtttt gggcaagctt
     1021 aattggatca attttcttcc tttctgtctg ctggtatctc tgcagttcat actccatttc
     1081 ggctttcaat tcggtttcgc tgactgtcag attttctgct ttggcaattt cgatatgaac
     1141 cagggaattt tccagattca gaatggctct ttttctgtac gtttccttca tctgatccag
     1201 gctgatttca aattttttgg caaattcttc catactcata tgttcaagat tgttttcgtg
     1261 aatcgcatca tgaaaaatac tgttcatctc gtcgtcgatc atcgtttccg gaatctggat
     1321 ttcagactct tttcttacag cattaaaagc aagaattctg taatagctct gaagctgcct
     1381 ttccgcatat tccat
//
LOCUS       DAOQGJ010001329         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_73683_length_1394_cov_3.309186, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001329 DAOQGJ010000000
VERSION     DAOQGJ010001329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73683_length_1394_cov_3.309186"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1359
                     /locus_tag="PK683_17570"
     CDS             208..1359
                     /locus_tag="PK683_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10313.1"
                     /translation="MKFVNLFLILFFFLQTCRQNDVKDRKTVFDSVSLELKISDTHKN
                     YFTDALKNVYTGDEIKTFLNAMEEKSNPVCEMSWGKKKYDANVLHSFLRKENFTKPPF
                     SDFSKPGELLAKLISGCWNFFLMNDEFPQNDFEKLKSFYPNERKKCYSVAFMQPYIMH
                     SIVGCETLTFIDTDWKVLHAHRQMIEKFQQNRFTSEEEAKKALSEIKLSWLGNINPPR
                     AEEKIVNLDTFCKNQNAICLKSLLDFQKKFKDLKSVDFRLTYLHELDFTKDPGTVSMF
                     YMSNAIDPMYTNPSQFKSFLDRIKGFLGPEEKLVLMYHSGARPLYGIYELKNSGKEKL
                     DIEAKCRDRYLDPPTVQNQGYYYTKLDYYLTAKKGPPYCSARQVDKPKS"
BASE COUNT          470 a          268 c          282 g          374 t
ORIGIN      
        1 ggtctgtgtg atcattttta cgatgacaga cctaaacacg atcagattca ggaacttgcc
       61 tactggatct gggaagaaaa aagaagaccg gcatcaaaag atctggaaat ctggcttgag
      121 gcagaaaaaa ttcttcagga aagaatcagg gattactgaa aattttatct tgaggttact
      181 gactgcaaaa atttcgctgt atttgcaatg aaatttgtaa atcttttttt aatcctattt
      241 tttttcctgc agacctgcag acagaatgat gtaaaagaca gaaaaactgt atttgacagt
      301 gtaagtcttg aactcaaaat ttccgacacc cataaaaatt attttactga tgcactgaaa
      361 aatgtttata ccggtgatga aatcaagacc ttcctgaatg caatggaaga aaaatcaaat
      421 cctgtctgcg aaatgagctg gggaaagaag aagtacgatg ccaatgtact tcattcattt
      481 ctcagaaagg aaaactttac aaagcctccc ttcagcgatt tttcaaaacc tggggagctt
      541 ctggcaaaac tgatttccgg atgctggaac ttctttctga tgaatgatga atttcctcag
      601 aacgactttg aaaaactgaa gagtttctat ccgaacgaaa gaaaaaaatg ctacagcgtg
      661 gcatttatgc agccatatat tatgcactca attgtcgggt gtgaaacact gacattcatt
      721 gatactgact ggaaagtcct tcacgcacac agacagatga tcgagaagtt tcagcagaac
      781 cgatttactt ctgaagaaga ggcgaaaaag gcactgtccg aaataaaact ctcctggctc
      841 ggaaacatca atccgcccag agcagaagaa aaaatcgtca atttggatac cttctgcaaa
      901 aatcagaatg caatctgcct gaaaagtctt ctggattttc agaaaaaatt caaggatctg
      961 aaatcagtgg atttccggct cacatatctt catgaactgg atttcacaaa agatccggga
     1021 acggtctcaa tgttctatat gagcaatgca attgatccta tgtacaccaa tccttctcag
     1081 ttcaagtcat ttctggacag aattaaagga tttctcggac ctgaagaaaa actggtactg
     1141 atgtatcatt ccggtgcgag gcccttgtac ggaatttatg agctgaaaaa ttcaggaaaa
     1201 gagaaactgg acatagaggc gaaatgcagg gacagatatc ttgaccctcc tactgtacag
     1261 aatcagggtt actattatac taaactggat tactatctga cagccaagaa gggacctccc
     1321 tactgttcag caagacaggt cgataaaccg aaaagctgaa gtttttttct gatttgctga
     1381 tttacagatc agcg
//
LOCUS       DAOQGJ010001330         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_73688_length_1394_cov_3.200149, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001330 DAOQGJ010000000
VERSION     DAOQGJ010001330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73688_length_1394_cov_3.200149"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1137)
                     /locus_tag="PK683_17575"
     CDS             complement(226..1137)
                     /locus_tag="PK683_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNH10314.1"
                     /translation="MKSSILTHILLAALTLLTMTFHENLFLLPWLPADKVWLYFQEEI
                     LYSLSLFTILFAHEMGHYIPSRMYNVRATLPYFIPMPFGPIGTMGAVIKIKDEIPDKK
                     KLFDIGAGGPFMSLILSLPCWIYGIYHSKLIRFSGMESPERILIFGDSLFTYFTSQWI
                     LGPYDTALFDVSIHPLARAGWVGLIITAINLLPFGQLDGGHIIYSLFGERYRSWIYYL
                     FLGFLFLGFVSFTWIFWGFLIYYLIRVEHPFVSDAELDWKRKILGIFLLFSLAFIFVP
                     VPMVSLAELKEHNLLEFLVYKMYSLGR"
BASE COUNT          460 a          302 c          298 g          334 t
ORIGIN      
        1 ctcactgtgg gaactgcttc ataccatcca ggacccgact gcaaaaaact tcagggcagt
       61 cttggacaat gacagatctt tcttcggccg cgatacagaa catatgaaaa gggagctgaa
      121 cgaaatcatc cgtcacaacc tgatcggaaa cagagaaatc gcttctaact gaaagcgact
      181 gactgaaagc gacgtttcga aacgtcgcca aactttttct cccctctatc tccccagcga
      241 atacatctta tagacaagaa actccagcag attatgttcc ttcaattccg ccagtgaaac
      301 catcggaacg ggcacaaata tgaatgccag tgaaaagaga agaaatatgc ccagtatctt
      361 ccttttccag tccagttctg cgtcagaaac aaacggatgt tccacccgga tcagataata
      421 gattaaaaaa ccccagaata tccaggtaaa acttacaaac cctaagaaca gaaatcccag
      481 aaaaagatag tatatccagg atctgtatct ttcaccgaaa agtgaataga tgatatgccc
      541 cccgtccagc tgaccgaaag gaaggagatt gatggcagtg atgatcagtc ccacccagcc
      601 agcgcgtgcc agcggatgaa tgctgacatc aaacagggcg gtatcgtacg gccccaaaat
      661 ccactgagaa gtgaaataag tgaaaagact gtctccgaaa atcagtatcc tttcaggact
      721 ttccatcccg ctgaaccgga tcagtttcga atgataaata ccgtaaatcc agcagggaag
      781 cgacagaatc agactcataa atggcccgcc tgcaccaata tcaaaaagtt tttttttgtc
      841 agggatctca tccttgattt tgatcacagc tcccatcgtt ccgatcggcc cgaaaggcat
      901 cggaatgaaa taaggaaggg tggctctgac attgtacatt cgggaaggaa tgtaatgccc
      961 catttcatgg gcaaaaagga tggtaaaaag acttaaagaa tacagaattt cttcctgaaa
     1021 atagagccag actttgtctg caggaagcca gggcagcaga aagagatttt catgaaaagt
     1081 cattgtaagc agagtaaggg ctgccagtaa aatatgagtg agaattgaac ttttcacttg
     1141 tgctaaaatt ctatggaata atatttagtc aagaaccaaa agacagaacc gtttttggtc
     1201 aatctgccgg gtttttttta cgataaggta ctatatgttc agcttaaaaa atgtgagtgt
     1261 gaaaggcaaa ataatcttct cagtcggaat attccagatg ctgagtttaa tcattgcagt
     1321 tttagggacg cttggtctct ccaaaattga aagggaaatg atcgatttag ctgaatacaa
     1381 tcttgtagtc ggcg
//
LOCUS       DAOQGJ010001331         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_73697_length_1394_cov_2.914862, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001331 DAOQGJ010000000
VERSION     DAOQGJ010001331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73697_length_1394_cov_2.914862"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PK683_17580"
     CDS             <1..911
                     /locus_tag="PK683_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10315.1"
                     /translation="ALYVSRNPGHFRKSSKEEAVSQSKTYFTKAVELSKKKKYKEAKA
                     SYEEALELFANGEYYYEYGNTLSNIPDLKGSVEAYKIAEKLSYKRPELVLYNIACSYS
                     RMNKAEDGYKYLALAVDRGYNAFKYIQKDPDMENLRKRSDWEERIQELVPKSVKFKEK
                     DFVGALEVPGPRSPDEYFLCSNGTVLEKSICEKGFYRGKWKLENGDINVSWEDDCREV
                     GVGKIVSDLDCRVYEKYVFKACRKPDSDRSEKTSILGKTDIYYMKGILKSSEEGYGSP
                     VLKSNVKEPKVCDPDFKPKNSKELSW"
     gene            908..1207
                     /locus_tag="PK683_17585"
     CDS             908..1207
                     /locus_tag="PK683_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10316.1"
                     /translation="MRLLLLLILSFLLFRCNMKIEPMDPSSRQSLEDLVTDKTETKGQ
                     SNPSQNGLGSSSAIPQGTNGSNWINGNGTSSGSNGLNTGSGIETTFGGTTGSNSF"
     gene            complement(1204..>1394)
                     /locus_tag="PK683_17590"
     CDS             complement(1204..>1394)
                     /locus_tag="PK683_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10317.1"
                     /translation="YQKVTVFTQKGDLYYINCPKCEIKLRIRSIGNHACPSCKTRFTF
                     KPDLKTEVESEYQMLSLD"
BASE COUNT          457 a          247 c          330 g          360 t
ORIGIN      
        1 gggcactcta tgtcagcagg aatccaggac atttcaggaa gagttcaaaa gaggaagccg
       61 tcagtcagtc taaaacttat ttcacaaagg ccgttgaact gagtaaaaag aaaaaataca
      121 aggaagccaa ggcttcatat gaagaagctc tggagctgtt tgcaaacggc gaatattatt
      181 atgaatatgg aaatacttta tcaaacattc ctgatttaaa ggggtctgta gaagcctata
      241 agatagcaga aaaactgtca tataaaagac cggaactcgt actctacaat atcgcctgtt
      301 cttacagcag aatgaacaag gcagaggacg gatacaagta tcttgcactg gctgttgaca
      361 gaggatacaa tgcatttaaa tatatccaga aagatcccga tatggaaaac ctcaggaaac
      421 gttctgactg ggaagaacgt attcaggaac ttgttcctaa aagtgtaaaa ttcaaggaaa
      481 aggattttgt cggggctctt gaggttccag gaccaagatc tccggatgaa tatttccttt
      541 gttcaaacgg aactgtattg gagaaatcaa tctgtgaaaa aggattttac agaggaaaat
      601 ggaagcttga aaacggagat attaatgtgt cctgggaaga tgactgccgt gaggtcgggg
      661 tagggaagat tgtatctgat cttgactgca gagtttacga gaagtatgtt tttaaagcct
      721 gtagaaagcc tgattctgac aggtcggaga aaacttcaat tctggggaaa acggatattt
      781 actatatgaa aggaattttg aaaagctccg aagaaggata cggctctcct gtactgaaat
      841 cgaatgtaaa agaacctaaa gtctgcgacc ctgacttcaa accgaagaat tcgaaggaac
      901 tgtcgtggtg agacttctac tattactgat cctttcattt ctgctgtttc ggtgcaatat
      961 gaagattgaa ccgatggatc caagctccag acagtcgctt gaggatcttg ttacagacaa
     1021 gacagaaaca aaaggtcagt caaaccccag tcagaatgga ctggggagct cttctgcaat
     1081 acctcaggga acgaacggat caaactggat caatggcaat ggaacaagtt caggttctaa
     1141 cggccttaat accggaagcg ggatagagac tacttttggg ggaacgacag gttcaaattc
     1201 cttttaatcg agagaaagca tttgatactc tgattccact tctgtcttaa gatcaggttt
     1261 aaaggtaaat ctggtcttgc aggaaggaca agcatggttt cctatggacc gaattctcag
     1321 cttgatttcg catttaggac agttgatata gtacagatcg cccttttgag taaatacagt
     1381 gactttctgg tatt
//
LOCUS       DAOQGJ010001332         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_73727_length_1394_cov_2.194922, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001332 DAOQGJ010000000
VERSION     DAOQGJ010001332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73727_length_1394_cov_2.194922"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PK683_17595"
     CDS             <1..476
                     /locus_tag="PK683_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10318.1"
                     /translation="LQSISAQQAVKVIGGVTAKIENNSLVSFNKSGILKSAKDPETMG
                     GDWEYNSWRVTKGGGKINFFFNAKDLPKGKQIFIHLVYAGWGDDTRINLRLNGEKITT
                     NRPVSGHAWADADHGIFEITDLLKRLNAEEYTAELELDPSSPMVLFFKSFALHSY"
     gene            664..>1394
                     /locus_tag="PK683_17600"
     CDS             664..>1394
                     /locus_tag="PK683_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10319.1"
                     /translation="MEFDPKLGIQNIESFWKLEAEFGEERPAHDVYLDEIVSDRYSLI
                     RGMQVIRDELQLAGLDKKSDIAACGADFSLPSVITTLAHTNCGDRIHQGEATTAYIQM
                     IASRFATISEIGEYKLEAFAPTGGGTDDGATFAHVTWAHHIDDVLREQIYLGSASSYV
                     MCNFDLKTHVGRMEKDGIVIGKTSESKWRYPRAACGAVVGCLAHYNDGNAVHRRIRSD
                     LTEKGYAHLKEHPVMAGNTDVTSAVA"
BASE COUNT          419 a          293 c          333 g          349 t
ORIGIN      
        1 ttctccagtc aatttccgca cagcaggcgg tcaaagtgat tggcggtgtt actgcaaaaa
       61 ttgaaaacaa ttccttagtc agtttcaaca aaagcggcat tttgaaatca gccaaagatc
      121 cggaaacaat gggcggtgac tgggagtaca attcctggag ggtaaccaag ggcggcggga
      181 agataaattt tttctttaac gcaaaggacc ttcccaaagg aaaacagatt tttattcacc
      241 tggtttatgc aggctgggga gatgatacca gaatcaattt acgcttaaac ggagaaaaaa
      301 ttaccacgaa caggcctgtt tcaggtcatg cctgggcaga tgcagatcat gggattttcg
      361 aaattactga tcttctgaaa cggctgaatg cagaagaata tacggcagaa ctggagcttg
      421 atccgtcctc ccctatggtg ctttttttca agtcctttgc acttcattca tactgaatca
      481 gatgctgaat aataacggat tttcataaat tgtattatta tccagtgaca taacacaatg
      541 gcagacctga gagtctgctg aattcaggct ggctcttccg gctccgtaat tctccaagag
      601 cttgacattt tacctgcacc gtaaaaaact gcagggacaa tttatcacaa tatagaggca
      661 gcaatggagt ttgatcctaa acttggaata cagaatattg aaagtttctg gaaactggaa
      721 gcagaattcg gagaagaaag accggcacat gatgtatatc tggatgagat agtcagtgac
      781 agatacagtc tgattcgcgg aatgcaggtg attcgggatg aactgcagct cgcaggtctg
      841 gataaaaaat ccgatattgc tgcctgcgga gcagatttca gccttccttc tgtcatcact
      901 acattggccc atacaaactg cggagacagg attcatcagg gagaagccac tacagcatac
      961 atacagatga ttgcatcccg atttgccacg atctctgaaa tcggcgaata caaactggaa
     1021 gcatttgccc ccacaggagg aggcactgat gacggtgcaa catttgcgca tgtgacctgg
     1081 gcacatcata tagatgatgt attgcgcgaa cagatttatc tcggctcagc ctcctcttat
     1141 gtaatgtgca attttgatct gaagactcat gtcgggcgta tggaaaaaga cggaatcgtc
     1201 ataggaaaga caagtgagtc caaatggaga tatcctagag cagcctgcgg cgctgttgta
     1261 ggatgtctcg cccattacaa tgatggcaat gctgtacaca gaagaatccg ttccgatctc
     1321 acagagaaag gatatgctca tctgaaagaa catccggtca tggctggaaa tacggatgtc
     1381 acttcagcag tggc
//
LOCUS       DAOQGJ010001333         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_73908_length_1392_cov_2.341062, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001333 DAOQGJ010000000
VERSION     DAOQGJ010001333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73908_length_1392_cov_2.341062"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..633)
                     /locus_tag="PK683_17605"
     CDS             complement(127..633)
                     /locus_tag="PK683_17605"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNH10320.1"
                     /translation="MIAAADVHYREDKAVGVIVLFTEWESESIHSFEKIEFTGKYSEY
                     IPGEFYKRELPILLSLHEKIKDAALEAFIVDGYVYLGLEEKPGLGYYVYQKLGGSIPV
                     IGAAKSRFKDNSENCRELYRGKSTKPMYITSVGISSEDALRKMQKMKGNFRIPDILST
                     LDRMTKEF"
     gene            complement(635..>1392)
                     /gene="asd"
                     /locus_tag="PK683_17610"
     CDS             complement(635..>1392)
                     /gene="asd"
                     /locus_tag="PK683_17610"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679241.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNH10321.1"
                     /translation="KAFAKMYKINVSEAELNIQEYSSLNEFFTRALKAGARIIDSAEN
                     AAVSPVDARITSFGEINEETLIQAKGIDYSLKELLGSEKYYPFFENGKFITFYLSPQD
                     YHRIHSPFYGQILGYYYAPGKLFPVNDMAVLGIKSLFPKNERLITFLQTEYGKIAVVK
                     VGASNVGKIRVTYDTKIVTNSWIRLPKEYEYTNVNIFIDKGSELGRFEMGSTVILVFE
                     KDSIDLVGLKSDEKCQYGSTIGYFRKKQINLPK"
BASE COUNT          363 a          306 c          259 g          464 t
ORIGIN      
        1 aattccagct tctttcttcc atttatttcc tggaaggaaa acttcacaat ctggatgaag
       61 ctaaagaaag aaaaattgcc cgtcagcttc aaggacttgg tcagtaatct cgcgaatgca
      121 gtttcgctaa aactcttttg tcatcctgtc gagtgtcgac aggatgtcag gtattctgaa
      181 attccccttc attttctgca ttttccgcaa agcatcttct gatgaaattc caacagatgt
      241 gatgtacatc ggttttgtgc tttttcctct gtaaagttct ctgcagtttt ctgaattatc
      301 cttgaacctg ctttttgcag ctccaattac aggaatggaa cctccgagtt tctggtatac
      361 ataatatccc agtccaggtt tttcttccag accaaggtaa acatatccgt ccacaatgaa
      421 tgcttccagt gcggcatctt ttattttttc atggagagac agaagaatag ggagctctct
      481 tttgtaaaac tccccgggaa tgtactctga atacttcccg gtaaattcta ttttttcaaa
      541 tgaatgaatg ctctcagatt cccattccgt aaatagaaca ataacaccga cagccttgtc
      601 ttctctgtag tgaacgtcag ctgcggcgat caaattactt aggaaggttt atctgtttct
      661 ttctgaagta acctatcgtg cttccatact gacatttttc atcagatttc agaccgacca
      721 gatcaataga gtctttttcg aaaaccagga tcactgttga acccatttca aaccttccca
      781 gttcggatcc tttgtctata aatatgttta catttgtata ttcatattct ttgggaagtc
      841 tgatccagga atttgtaact atctttgtat cataagtgac cctgattttc ccgacgttgg
      901 aggctccgac ttttacgact gcaatttttc catactctgt ctgcaggaag gtaatcagtc
      961 tttcattctt ggggaacagg ctcttgatcc cgagaactgc catatcattc acgggaaaga
     1021 gttttcctgg cgcataatag tatccgagta tctgaccgta aaacggggaa tgaatcctgt
     1081 gataatcctg aggcgagaga taaaatgtaa tgaattttcc attttcaaaa aagggataat
     1141 atttctctga tccgagaagt tccttcagac tgtaatcgat tcccttggcc tggatcagtg
     1201 tttcttcatt gatttctccg aagcttgtga ttcttgcatc tacaggggat acagctgcat
     1261 tttctgcaga atcaatgatc cttgcacctg ccttcagcgc tctcgtgaaa aattcattca
     1321 gggaactgta ttcctgaata ttgagttcgg cctcgcttac attgattttg tacatctttg
     1381 caaatgcctt ca
//
LOCUS       DAOQGJ010001334         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74172_length_1389_cov_2.328336, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001334 DAOQGJ010000000
VERSION     DAOQGJ010001334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74172_length_1389_cov_2.328336"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="PK683_17615"
     CDS             complement(<1..1300)
                     /locus_tag="PK683_17615"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNH10322.1"
                     /translation="MTEIQNKKSYHKIYPNLNVLRKHSNGFKSHLKKYFAAKSFLTPD
                     LIIQYSRNLISENQSVRKEVSDEIRFLTVDECQDSDPVQLDLFEKLFADRKSGLIFVG
                     DPKQSIYRFRNADSVNYNDFVNKMRFRKNTLNETRRSAPGLAEVFNRMFVDAGIGYSP
                     VTAHRKKNPCSAPSLLLLGLDEKNAPLEDGEWRAADLRENAADEICSAIEKITDNPEF
                     LTESEDSTETELKTRKISCSDIAVLSSSNSDLQFLLKKMTQKGIPASIYKSEIFYEDR
                     LVFAYSCILNAVENPADTDSLYKALVSDLFLFPEDELSFYSLKFQENSNNDELNKIYS
                     VLRDAYHESLSSPVSGILKKIAVRTGFYFRISIGAEGRRNLTNCYHLLEILDQLQFGG
                     NLSFSETVRTFRRNIEKGKSQALKLDNLGDVFSPDSSSVRL"
BASE COUNT          376 a          296 c          271 g          446 t
ORIGIN      
        1 tcagacggac gcttgaagaa tcaggagaga agacatctcc tagattgtcc agcttgagtg
       61 cctggctttt ccctttttct atgtttctcc taaaagtacg gacagtttcc gaaaaactca
      121 ggtttccccc gaactgcagc tgatccagaa tttccagaag atgatagcaa tttgtcaaat
      181 ttcttctgcc ctctgctcct atggaaattc tgaaataaaa acctgttctg actgcaattt
      241 tcttcagtat ccccgaaacc ggactggaaa ggctttcatg ataggcgtcc ctcaagacag
      301 aatatatttt atttaattca tcattattag agttttcctg gaacttgagg ctgtaaaagg
      361 acagctcgtc ttccgggaaa agaaaaagat cagaaactag tgccttgtac agagaatctg
      421 tatctgcagg attttccact gcattcagaa tgcagctgta tgcaaaaaca agtctgtctt
      481 cgtaaaaaat ttcgctttta tagatggatg caggaattcc tttttgggtc atttttttca
      541 gaagaaactg aagatctgaa tttgaagaag aaagaacagc aatatccgag cagctgattt
      601 ttctagtctt cagttcagtc tccgttgaat cctccgattc agtcagaaat tcaggattgt
      661 ctgtgatttt ttctatcgca ctgcatattt catctgctgc attttccctc agatcggctg
      721 ctctccattc accgtcttct aaaggtgcgt ttttttcatc cagaccaagg agaagaaggc
      781 ttggggctga acagggattc ttttttctgt gagccgtaac aggagaatat ccgatacctg
      841 catcgacaaa cattctgttg aacacctctg caagtcccgg agcacttctt ctggtttcgt
      901 tcagggtgtt ctttctgaat ctcatcttat ttacaaaatc attataattt acagaatctg
      961 catttctgaa tctgtaaatg gactgcttcg gatcgcctac gaaaatcagt ccgctttttc
     1021 tgtccgcaaa aagtttttca aaaagatcca gctgtaccgg atccgaatcc tgacactcgt
     1081 ccactgtcag aaaacgaatc tcgtctgaaa cttcctttcg gacagactga ttttcagaaa
     1141 taagatttct ggaatactga atgatcaggt cgggagtcag aaaacttttt gccgcaaaat
     1201 attttttcag atgagacttg aatccgttgc tgtgttttct cagcacattc agattaggat
     1261 agattttatg gtaggatttt ttattctgaa tttcagtcat ccgatttctg agctcttcct
     1321 gttccccgtc cgacctgcag attcggtcaa ggtaatattt tgcagggaga gaatctgtct
     1381 ttttcaaaa
//
LOCUS       DAOQGJ010001335         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74272_length_1388_cov_2.564141, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001335 DAOQGJ010000000
VERSION     DAOQGJ010001335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74272_length_1388_cov_2.564141"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK683_17620"
     CDS             complement(<1..183)
                     /locus_tag="PK683_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10323.1"
                     /translation="MKNKKNIELALGINSTMTANCRQTGEFWARNLSSAFQTSYCAKT
                     DFLGTASNANFYLEKGI"
     gene            341..976
                     /locus_tag="PK683_17625"
     CDS             341..976
                     /locus_tag="PK683_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10324.1"
                     /translation="MTKQIFALENISTETFFSEIRNIGKSTHAVQLILNSKREKKGEF
                     LLSWRSFHPERNWEEWKWRGSVKKTVNSELELIPKNCTLFSQKNGASRQIPVRTIDCE
                     HYKMTLKKTEGNSAVLSPSLSGEDSAVLLNSETSETACLAVSEEEDRVLGWCSEARYI
                     TKSSSARVQKSGLSAEILESIDSTVWFKKTPVIKKGDIIIINIPAYRPEIY"
     gene            complement(970..>1388)
                     /locus_tag="PK683_17630"
     CDS             complement(970..>1388)
                     /locus_tag="PK683_17630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH10325.1"
                     /translation="YLLKAYTLERQKSRILEGLHILVAEDNELNQKLVTKYLTGQGAS
                     VKLALNGIKCLELLNTARFDLILMDLQMPEMDGYEASKTIRNSYRIPIPIIACTADTM
                     AGEKEKCFQAGMNGYISKPYSEDELINAILSVCSVQ"
BASE COUNT          430 a          283 c          273 g          402 t
ORIGIN      
        1 gatgcctttt tccagataaa aatttgcgtt tgaggcagtt cccagaaaat cagtctttgc
       61 gcagtatgat gtctgaaatg cactggaaag atttcttgcc caaaactctc ctgtctgtct
      121 gcagtttgct gtcattgtgg aattaattcc taatgcaagt tcaatatttt tcttattttt
      181 catctttgat ttttttactg cagacggaga acactcaaaa atcagaaatg aaaaaattat
      241 tgcagagata gaggaaaaac aacagccttt ccaaggagaa ttctttatga accgcatcat
      301 tctttcacct gctggaatta ttgtaatata cctgattttt ttgacaaagc aaatttttgc
      361 cctggagaat atttcaacag aaactttttt ctccgagata agaaacatcg gcaaaagcac
      421 tcacgctgtt cagctgatac tgaactcaaa gagagaaaaa aaaggagaat ttcttctcag
      481 ctggagaagt tttcatccgg aaaggaactg ggaagaatgg aaatggagag gttcagtaaa
      541 aaagacggta aattcggaac ttgaactcat tcctaaaaat tgtacacttt tttcgcagaa
      601 aaacggtgcc tcccgacaga tccctgtcag aacaattgac tgcgaacatt ataaaatgac
      661 tctgaagaaa actgaaggca attcagcagt tctttcccct tccctgtcag gcgaggattc
      721 agcagttctc ctcaattcag aaacctcaga gaccgcctgt cttgcagttt cagaggaaga
      781 ggacagagtt cttggctggt gttctgaagc caggtatata acgaagagtt ccagtgccag
      841 agttcagaaa agcggactga gtgcagaaat tctggaatca atagacagca ctgtctggtt
      901 caaaaagact ccagtcatca aaaaaggaga tattataatt ataaacatac ctgcatacag
      961 accggaaatc tactgaacag agcagacaga aagaattgca ttgatcagct catcttcaga
     1021 atagggtttt gagatatagc cgttcatgcc tgcctgaaag catttttcct tttctccggc
     1081 catcgtatct gcagtgcagg caatgatcgg aataggaatt ctgtaactgt ttcgaattgt
     1141 ctttgatgcc tcatatccgt ccatttccgg catctgcaga tccatcagaa tcagatcaaa
     1201 tcttgccgta ttcagaagtt ccagacattt gatcccgttc aatgcaagtt ttactgaagc
     1261 cccctgacca gtcagatatt ttgtcacaag cttctgattg agttcattgt cttctgcaac
     1321 gagaatatga agaccttcca atattctgct tttctgtctt tccagtgtat atgccttaag
     1381 aagataag
//
LOCUS       DAOQGJ010001336         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74279_length_1388_cov_2.395349, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001336 DAOQGJ010000000
VERSION     DAOQGJ010001336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74279_length_1388_cov_2.395349"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /gene="pyrF"
                     /locus_tag="PK683_17635"
     CDS             complement(<1..474)
                     /gene="pyrF"
                     /locus_tag="PK683_17635"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNH10326.1"
                     /translation="MSFVQRLAEYSRNRRTLLCVGLDPDRKKIPDHLKKEKSSLSRFC
                     CETVEAVAPYCIAFKPNIAFFESSGYDGIRQFEDTIHFIKKKYPDHLIVTDAKRGDLG
                     NTAKEYAEYFFGQLKTDSLTVSPYMGRDSLEPFLNFKDSHLFALCLTSNPGSSDLQ"
     gene            complement(471..1085)
                     /locus_tag="PK683_17640"
     CDS             complement(471..1085)
                     /locus_tag="PK683_17640"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNH10327.1"
                     /translation="MKIVIIDNYDSFTFNLYQYAGEILQNRMTSFSLDVVRNDELSLE
                     EIAERKYDRIVISPGPGDPKDIEYFGVCSKVILELGRKTPVLGVCLGMQGIAHYFGGK
                     VVRAKIPKHGKTDRISHDGKGVFAGLPQQIEIMRYHSLVVDDASLPAFFEKSARVESS
                     ENDGELMGIRHKEYPIEGIQFHPESFATEGGMKMIENFIFGGSR"
     gene            complement(1082..>1388)
                     /locus_tag="PK683_17645"
     CDS             complement(1082..>1388)
                     /locus_tag="PK683_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNH10328.1"
                     /translation="FSGFAATFPAGTLSGSPKIESMKIIARTEKRPRGIYGGGIGQFG
                     FNGNCTFAIPIRSFFVCDSYGYAQASGGIVFDSVPENEYDEIKRKLAAVEKALEFFI"
BASE COUNT          363 a          348 c          279 g          398 t
ORIGIN      
        1 ctgcagatcg gaggatccag ggtttgaagt aaggcagagt gcaaaaagat gactgtcttt
       61 gaaattaaga aaaggctcaa ggctgtctct gcccatatag ggagaaacgg taagggagtc
      121 cgtctttaat tgaccgaaaa aatactcagc atattctttt gcagtattgc ccagatcgcc
      181 tcttttagca tcagttacga tcagatggtc agggtatttc tttttaatga aatgtattgt
      241 gtcctcaaac tgcctgattc cgtcataacc ggatgattca aagaatgcaa tattcggttt
      301 gaacgcaatg cagtaggggg caacggcttc caccgtctcg cagcagaatc tgctcaatga
      361 agatttctct tttttcaaat gatcgggaat tttctttctg tctggatcaa gcccgacaca
      421 taagagcgtt cttctgtttc tggaatactc agccagtctc tgtacaaaac tcatctgctt
      481 cccccgaata taaaattctc tatcattttc attccgccct cagtcgcaaa agactcagga
      541 tgaaactgga tgccttcaat agggtactct ttatgtctga tgcccatcag ctcaccgtca
      601 ttttccgaag attccaccct tgcggacttt tcaaaaaaag caggcagact cgcatcatcc
      661 acaacaagtg agtggtatct cataatttca atctgctggg gaagcccggc aaatactccc
      721 ttcccgtcgt gagatatcct gtccgtcttg ccgtgtttag ggatttttgc ccttacaacc
      781 tttccgccga aatagtgtgc aattccctgc atccccagac agactcccag aacaggtgtt
      841 tttctgccga gttccagtat cacttttgag cagactccga aatattctat atccttcgga
      901 tctcccggac cgggcgatat gactatcctg tcgtatttcc tttctgcaat ttcctccaga
      961 ctcagttcat cattacgtac aacgtcaaga ctgaacgacg tcatcctgtt ctggagaatt
     1021 tcccctgcat actgatacag attgaatgta aatgaatcat aattatctat aattacaatc
     1081 ttcatataaa aaactccagt gccttctcta ctgcggcaag ctttctctta atctcgtcat
     1141 attcattttc aggcaccgag tcgaatacaa tacccccgga agcctgagcg tatccgtaac
     1201 tgtcgcatac gaagaatgaa cggataggga tggcaaatgt gcagttcccg ttgaacccga
     1261 actgacctat gcctccgccg tatatgcccc tcggacgttt ttccgtcctt gcgatgattt
     1321 tcatggactc aatcttcgga gagccggaaa gtgttcctgc tggaaacgtt gccgcaaagc
     1381 cggaaaac
//
LOCUS       DAOQGJ010001337         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74355_length_1387_cov_2.820571, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001337 DAOQGJ010000000
VERSION     DAOQGJ010001337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74355_length_1387_cov_2.820571"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PK683_17650"
     CDS             complement(<1..679)
                     /locus_tag="PK683_17650"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH10329.1"
                     /translation="MENEVRKQFIFSFKSVYFSVSLSSVTEVIESPEIIQVPIQEKGL
                     LGILKYKDTPVPVIDPAETGKIRTSGDIPVPKSIVLTESDESRIGFVMDRFIRIIADA
                     ESGEESGSEPEEFVSGFSILEKIPLVILNEKAIAEHYKKIFRKQISVQKKNTEISTVN
                     VQNRSETEKFIFFSIGEVQFGVPIREVLEVLENVDVTPLFKTVPALRGVINVRGRVIA
                     CLDISSYI"
     gene            complement(790..>1387)
                     /locus_tag="PK683_17655"
     CDS             complement(790..>1387)
                     /locus_tag="PK683_17655"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNH10330.1"
                     /translation="KGTGKERTSALLKDLHQIYEVYYDIYLLNQKGVICAAAIRQDLI
                     GQDQSDRDWFQQAVQGNVSFTDMYKSDTAGAYTVSYCAPVKNKKGQIVGVLTTRFNWN
                     FAVDIINASLVYSDCKVYLVNSSGLLIASTDGRDILKKDFSRFEAFKKAIDGNNGFII
                     ERDSDTNNIFLIGYARTEGYNRYPGKGWCALIMQQKTF"
BASE COUNT          349 a          345 c          275 g          418 t
ORIGIN      
        1 ctatatagga agaaatatcc agacaggcaa tgacccttcc tctcacattg atcactcccc
       61 tgagggcggg aacagtcttg aaaagaggcg tcacatccac attttccaga acttccagaa
      121 cctccctgat cggaacaccg aactgaacct ccccgatgga aaagaagatg aatttttccg
      181 tttccgatct gttctgcaca ttgacagtgc tgatctcagt gtttttcttc tgcacggaga
      241 tctgttttct gaatattttt ttataatgtt ccgctattgc tttttcattc agaatgacaa
      301 ggggaatctt ttccagaatg gaaaacccgg atacaaactc ttccggctct gatccggact
      361 cttccccgct ttccgcatcc gcaatgattc tgatgaatct gtccatcaca aatccgattc
      421 tggactcatc cgactcagta agcacaatgc ttttaggaac tggaatatcc cctgaggttc
      481 ttatctttcc tgtctctgca ggatcaatga caggaacagg agtgtccttg tatttcagta
      541 ttcccagaag tcctttttcc tgaatcggca cctgaatgat ttcaggagac tctatgacct
      601 cagtgacgct cgaaagactg acggaaaaat agaccgactt gaaagagaag ataaactgct
      661 ttctcacttc attttccatg acagttttcc tgcctcttcc gtaaggaaag atccgagaaa
      721 cattccgtgt tcaagatgat aaacgatttt atatatttac agttcatctg gttctccgcc
      781 gttatctgcc taaaaagtct tctgctgcat gatcagagca caccagcctt ttccgggata
      841 tctgttgtat ccttccgtcc tggcatatcc tatgagaaag atattgtttg tatccgaatc
      901 cctttcaatg atgaagccgt tgttcccgtc gattgccttt ttgaatgctt caaaacggga
      961 gaaatccttt ttcagaatat cccttccgtc cgtggatgcg atcagaagtc ctgacgaatt
     1021 gaccagatac accttgcagt cgctgtagac cagagatgca ttgatgatgt ccactgcaaa
     1081 gttccagttg aacctggtag taagcacgcc tactatctgt ccctttttgt ttttgacagg
     1141 agcacagtag gatacagtgt atgctcctgc cgtatctgat ttgtacatgt ctgtaaagga
     1201 tacgtttccc tgcacggcct gctgaaacca gtctctgtcc gactggtcct gtccgatcag
     1261 atcctgacgg atcgcagctg cacagatcac tcctttctgg ttcagaagat agatatcata
     1321 atagacttca tagatctgat gaagatcctt cagaagtgcc gatgttcttt cctttccggt
     1381 tcccttc
//
LOCUS       DAOQGJ010001338         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74394_length_1387_cov_1.781532, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001338 DAOQGJ010000000
VERSION     DAOQGJ010001338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74394_length_1387_cov_1.781532"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..360
                     /locus_tag="PK683_17660"
     CDS             178..360
                     /locus_tag="PK683_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10331.1"
                     /translation="MNSAASLFMTEHKSWLEEFRSDEFQKKTEQWKNIFPEGAGYLTL
                     EQNRPQETAHPKLPML"
     gene            389..817
                     /locus_tag="PK683_17665"
     CDS             389..817
                     /locus_tag="PK683_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10332.1"
                     /translation="MKVKLMTVIQYYRDGRPNTSKEFKNPSWDLVEKSIKQMDNYCFP
                     IVWLHPSEDPDDEKTLDIIGGNGRWAVLHHMGDWQHSDGSGDETEARLWESDQGYECQ
                     EQNVIKDIETVLKYVRVFYDTASYAKLKQVNTQTKAKKKK"
BASE COUNT          451 a          265 c          276 g          395 t
ORIGIN      
        1 cctcagtgcc gcatctgtac agaatataaa gaaatcagat tatcataaaa gtttttatta
       61 aaaattttat tgtctgcgtt tcagttcttc ctgattcagt atgtctgatg aatcataaaa
      121 gctttcaaaa agaatcggga cagtcctgga gaatgccgaa gagactgtgt catcagagtg
      181 aattcagctg caagtctgtt tatgactgag cataaatctt ggctggagga attcagatca
      241 gatgagtttc agaaaaagac cgagcaatgg aaaaatatct ttcctgaagg tgcgggatat
      301 cttacactgg aacagaacag gccgcaggag acagctcatc caaaactgcc gatgctttga
      361 acctgaatct tatattagga atacactgat gaaagtaaaa ctgatgacag tcattcaata
      421 ttacagagac ggacgcccga atacttccaa agaatttaaa aatccttcct gggatctggt
      481 ggaaaaatca atcaaacaga tggacaatta ctgtttcccc atagtctggc ttcatccttc
      541 cgaagatcct gatgatgaaa agactctgga tattatcggc ggaaacggaa gatgggctgt
      601 tctgcatcat atgggtgact ggcagcacag tgacgggagc ggggatgaaa cagaagcgcg
      661 tctttgggaa agtgatcagg gatacgaatg ccaggaacag aatgtaatca aggatattga
      721 aactgtttta aaatatgtgc gcgtatttta tgatactgct tcctatgcga aactgaaaca
      781 ggtgaacact caaactaaag ccaaaaagaa aaaataacag actgtctgca gattacatca
      841 aagaagtctg tcaaatattt gaattcagag tataagagac tctctgtcaa aagatacaga
      901 gagatttttt catatttgac tgccttatct gatttatatc actccaatgg attccatatt
      961 cctgaaattt atgattgcac aataccgcaa tagcagcata ttctgtctaa aaatgaaaga
     1021 atgggactga tggggcgaag ctgtatacag actttgttca gatttccgag agaaagacag
     1081 cgcaccatat atgcaattca ctccggtttc gcttttttaa accgaactta aagaacaatc
     1141 tcgaactttt acttcgcttt tagtcttcct ctgacataaa tattgagatt cttcaaatgt
     1201 tatcatcttt tctatttccc ttttcagcat aaccgaaatt tttgcacgca aaaagaatcc
     1261 cttgagggca aactacaaat tttgcaccta tttgatttct ttcttgactc ccattttgca
     1321 tcgcacaata tcagttgtcc gacattttag cctcagccct gaatcgaacg aggattgatg
     1381 cttggga
//
LOCUS       DAOQGJ010001339         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74431_length_1386_cov_3.236664, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001339 DAOQGJ010000000
VERSION     DAOQGJ010001339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74431_length_1386_cov_3.236664"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PK683_17670"
     CDS             complement(<1..530)
                     /locus_tag="PK683_17670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH10333.1"
                     /translation="MNKKVKFELLKVGFCNHLECIAMRAGRVELVEFPALCGLIEHPE
                     KGFMLYDTGYSDHFEKAAEGFPECLYKYATPIQLPFEEKLEIQLHRRGIQRRDISYIL
                     ISHFHADHIAGLKDFPEAKFISSESEYRSLQKMNSFERLFRAVLSDLIPNDFERRAEF
                     METKKSFDLPRNLSPFT"
     gene            complement(563..>1386)
                     /locus_tag="PK683_17675"
     CDS             complement(563..>1386)
                     /locus_tag="PK683_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10334.1"
                     /translation="DALPGTNYTTSRNLVKSMIDTIAAKGAKINYESDWRFLNAIKTY
                     EPNGASDINNKNLLRYIKEDKGFEIDPHAHESTENYADVAYRISQLGVTPSANVGGFT
                     YNATSGSSTPWENMQNGLAGRINTSYTWKPTTLVLAGSADHKADDYSYGAWKPKSVSD
                     FYTHDSTKNLVFIGSGCSNVVTSTSAASDHISAITRISDGIKNGTYAKGKFYSANIMI
                     NQRDYSTDYITKVGQIIDGLKSRVDAKEIQWVFHSEKKTAWETVYSSQNFQLTCP"
BASE COUNT          352 a          280 c          291 g          463 t
ORIGIN      
        1 gtgaaagggg aaaggtttct tgggagatca aaggattttt ttgtttccat aaattctgct
       61 cttctttcaa aatcatttgg aatcagatcc gaaagaactg ctctgaaaag cctttcaaaa
      121 gagttcattt tttgaagaga tctgtactct gattccgatg aaatgaattt tgcttcagga
      181 aaatctttga gacctgcaat atgatctgca tgaaaatgag atattaagat ataagaaata
      241 tctcttcttt gaattcctct tctgtgaagc tgtatttcca gtttttcttc aaagggaagc
      301 tgtataggtg tggcatactt gtaaagacac tcaggaaatc cttctgctgc tttttcgaaa
      361 tgatccgaat accctgtgtc gtaaagcata aaaccttttt caggatgttc aatcagtccg
      421 cagagggcag ggaactcaac gagttccact ctgcctgctc tcatggcaat gcattccaga
      481 tgattgcaga aacccacttt cagaagttcg aattttactt ttttattcat gtaagtctat
      541 tgataaatat tcaagtcccg tgtcaaggac atgtcagctg aaaattctga gaagaataga
      601 ctgtctccca ggcggttttc ttttcgctgt gaaagaccca ttgaatttcc tttgcatcca
      661 ctctggattt gagtccgtcg atgatctgac ccacttttgt gatatagtcc gtgctgtagt
      721 ctctctggtt gatcatgata tttgctgaat aaaatttgcc ttttgcatag gttccgtttt
      781 tgatcccgtc tgaaattctt gtgatggccg aaatatgatc actcgctgcc gatgtggatg
      841 tcactacatt gctgcacccg cttccgatga agacaagatt ctttgtgctg tcatgagtgt
      901 aaaaatcaga cactgatttg ggtttccatg caccgtaaga ataatcatct gccttatgat
      961 ctgcactccc tgcgagaaca agagttgtag gcttccaggt ataggaggta tttattcttc
     1021 ctgcaagtcc attctgcata ttttcccagg gggtcgaact tcctgaagtg gcattataag
     1081 tgaatccgcc tacattggca gacggagtga ctcccagctg ggatattctg tatgccacat
     1141 ccgcataatt ttccgtgctt tcgtgcgcat gaggatctat ttcaaaacct ttgtcttctt
     1201 ttatgtatct taacagattc ttattgttta tgtcggatgc accgttaggt tcataagtct
     1261 tgattgcatt cagaaaacgc cagtcagatt cgtaattgat tttggcgccc ttggcggcga
     1321 tggtatctat catcgatttt accaggttgc gcgaggttgt gtaatttgtt ccaggcagtg
     1381 catcat
//
LOCUS       DAOQGJ010001340         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74438_length_1386_cov_3.090909, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001340 DAOQGJ010000000
VERSION     DAOQGJ010001340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74438_length_1386_cov_3.090909"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PK683_17680"
     CDS             <1..323
                     /locus_tag="PK683_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10335.1"
                     /translation="RGLSDSRSVSTVWRSQIYSANIPIPGDFNYRTVSDVQGNSWFAA
                     WILRLTESRSLKLSWFSRILTYDPQRSETKEYGTFFSVKPMGPFSKVQDSINALGLEF
                     QFKF"
     gene            389..>1386
                     /locus_tag="PK683_17685"
     CDS             389..>1386
                     /locus_tag="PK683_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10336.1"
                     /translation="MLIIIAKNIEPSLWTWPEFSSMTFVWVFAILMSALLAYLVVNYV
                     YRRQVMNNAAREMVISYARKRSLDQKEFLLIDEFLTSLTEKGYDIAEVFMNKGHLHST
                     MFEFFAKDKRHPEDMHVRILDKLFPEKEQNHQVLSPADLREGEVCGIAVGQKHYLGTV
                     LKSQSNLLLLHIEGLKEVLNGKTVHIYVYRQILGGYEIEAKVIETEGMSIKAAFTGEI
                     QRKGDAHIMTFLSCSVSLVPFFFPDEFTDSTFEEEYTGITLKISDRSVLFEPTNVTAA
                     DDLARHELWDMNIMFPDGMSVSVKGKVMQSRNPRKFILKILQVSDEDRSTLMDKILS"
BASE COUNT          396 a          257 c          317 g          416 t
ORIGIN      
        1 acagaggtct ttcagattca cgttctgtct ctactgtgtg gcgttctcag atctattctg
       61 caaatatccc tattcctgga gattttaatt atagaacagt ttcggatgtg caggggaaca
      121 gctggtttgc agcgtggata cttagactga cagaaagcag gtctctgaaa ctgagctggt
      181 tttcgagaat actgacatat gatcctcagc gttcagaaac aaaggaatat ggtacctttt
      241 tcagcgtaaa accgatgggg cctttttcca aggttcagga ttccatcaat gcacttggac
      301 ttgagtttca gtttaagttt tgagggacaa aaaacctgtt tttttcagat cagaaaagcc
      361 gatattataa ttgcagtcag gagtatttat gcttataatc attgcgaaga atattgaacc
      421 gtctctctgg acctggcctg aattcagttc aatgacattt gtttgggtct ttgccatctt
      481 aatgtcggct cttttggcgt acctagttgt taattatgtt tacagaagac aggtaatgaa
      541 caatgccgca cgggaaatgg tcatcagtta tgccagaaaa agaagtttgg atcagaaaga
      601 gtttcttctg atagacgaat ttctgacatc cctcactgaa aaaggctatg atatcgctga
      661 agtctttatg aacaaggggc atcttcattc tactatgttt gagttttttg ccaaggataa
      721 acgacatccg gaagatatgc atgtgcgcat attggataag ctttttccgg aaaaagagca
      781 gaaccatcag gttctctctc cggctgatct gagagaaggg gaagtctgcg ggattgcagt
      841 cggtcagaag cattacctcg gcactgtact gaaatctcag tcgaatcttc ttctgcttca
      901 tatcgaagga ctgaaggaag tgctgaatgg taaaacagtt catatttatg tctaccgaca
      961 gatactcggc ggatatgaaa tagaagctaa agtcattgag acggaaggga tgagcatcaa
     1021 ggctgcattc actggcgaaa ttcagagaaa aggagatgct catattatga catttctttc
     1081 ctgttcggtc tctctcgttc ctttcttttt tccggatgag tttactgaca gtacctttga
     1141 agaagagtat accggaatca ctttgaagat atccgacaga tcagtacttt ttgaacctac
     1201 caatgtgact gctgcagacg atctggcaag acatgagctc tgggatatga atattatgtt
     1261 tcctgacggt atgagtgtca gtgtgaaagg gaaggtgatg cagtctagaa accctagaaa
     1321 atttattctg aaaatacttc aagtttctga tgaggacaga tcaactctaa tggacaagat
     1381 cctttc
//
LOCUS       DAOQGJ010001341         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74441_length_1386_cov_3.072126, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001341 DAOQGJ010000000
VERSION     DAOQGJ010001341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74441_length_1386_cov_3.072126"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK683_17690"
     CDS             <1..357
                     /locus_tag="PK683_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10337.1"
                     /translation="FTEGFDLFEDESVIGINLPGHTESQMGILFRDQEEKLNFLVGDA
                     VWGMDALLQDKKPSDLARNVFYNQEQYDQTFDSLRKLFFTPESPRLIPSHCNRTWKQI
                     QKEISDRKQSLASASL"
     gene            370..>1386
                     /locus_tag="PK683_17695"
     CDS             370..>1386
                     /locus_tag="PK683_17695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10338.1"
                     /translation="MKNSVTFREKLLLLKSFIRTRWFLKFSSAEKLRKYQNHKIQNYL
                     NSQVSEFEFYRSFPKDLSSFPVMEKEEMLRNFHRLNRFGIKLKDAERTALQAEQSRDF
                     SPTINGITVGLSSGTSGRRGVFLASSDERFSWAGIILAKVLSSDSLLRVLNPFASPLR
                     IAFFLRANSSLYTTVKSRRIQFEYFDMTKSLIHHVIRLLNWRPHILIAPSSLLSELAE
                     NYKEKFSALHFDQVISVAEVLDEHTKQKLEKVFRTKIDQIYQCTEGFLGNTCSYGSIH
                     LNEEFVFFEKEWIDEERFYPIITDFTRKTQGFIRFRLNDILKIEREPCPCGNPAERIL
                     SIEGR"
BASE COUNT          439 a          290 c          286 g          371 t
ORIGIN      
        1 ttcactgaag ggtttgatct gtttgaagat gagtctgtaa taggaatcaa tcttccgggg
       61 catacagaat cccagatggg aatcctgttc agagatcagg aagaaaaact gaattttcta
      121 gtcggagatg ctgtgtgggg gatggacgca ctgcttcagg acaaaaaacc ttcagatctt
      181 gccagaaatg ttttttataa tcaggaacaa tacgatcaga cctttgacag tctgaggaag
      241 ctgttcttta ctcctgaatc tccgaggctg attccgtcac actgcaatag aacctggaaa
      301 cagattcaga aagagatttc cgacagaaaa cagagtcttg catccgcatc cttatgaact
      361 gagatcatat tgaaaaattc agtcacattc agagagaaac tccttcttct aaagtcgttc
      421 atacgcactc gatggtttct gaaattttcc tctgcagaaa aacttcggaa ataccagaac
      481 cataaaatcc agaattatct aaacagccaa gtatctgaat tcgaatttta cagatctttt
      541 ccgaaagatc tttcttcttt ccccgtaatg gaaaaagaag aaatgctccg gaactttcac
      601 cgactgaaca gattcggaat aaaactaaaa gatgcagaaa gaactgcact tcaggcagaa
      661 cagagtcggg atttctcccc gacaataaac ggaatcactg tcggactttc gagcggcaca
      721 tcaggcagga ggggtgtctt tcttgcatct tccgacgaaa gattcagctg ggcgggaatc
      781 atacttgcca aggtgctttc ttccgattcg cttctgagag ttctgaatcc tttcgcatca
      841 cctctcagga ttgctttttt tctgcgtgca aacagcagcc tgtatacgac agtaaaaagc
      901 agaagaattc agtttgaata ttttgacatg acaaaatcat taattcatca tgtcattcgt
      961 cttctgaact ggagacctca tatactgatt gcgccttcct cgcttctttc agagcttgca
     1021 gaaaactata aggaaaaatt ttccgcactt cattttgatc aggtaatttc agttgcggaa
     1081 gtgctcgacg aacatacaaa gcagaaactg gaaaaagtct tccgaacaaa gattgatcag
     1141 atatatcagt gcacagaagg atttctggga aacacctgca gttacggaag cattcacctg
     1201 aatgaagaat ttgtattctt tgaaaaagaa tggattgatg aagaaagatt ctatccgatc
     1261 atcactgatt tcaccagaaa aacacagggg ttcattcggt tcagactgaa cgatattctg
     1321 aaaattgaaa gagaaccctg cccctgcgga aatcctgcag aacgaattct gtccatagaa
     1381 ggcagg
//
LOCUS       DAOQGJ010001342         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74522_length_1385_cov_3.049624, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001342 DAOQGJ010000000
VERSION     DAOQGJ010001342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74522_length_1385_cov_3.049624"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PK683_17700"
     CDS             <1..1194
                     /locus_tag="PK683_17700"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen synthase"
                     /protein_id="HNH10339.1"
                     /translation="LFKKEHFLNPPEKLVIMSSSPRREYNLKKFFSEYLPEKLLNKEI
                     IFEPVRGNLRTRARKWQDGTASGIVFAKAGIDRILIERFNESGDEELKLERDFFHSLL
                     NDCLFLVLPLSSNPNAPAQGGIAVEIRKGRDDLRTFLKKMTIKNVDNCIRGEREELMK
                     YGGGCHQKIGVSILQRDYGTVMILKGETDDGKTLDSKKLFRIHSPKTSDLSGIWPLRN
                     EGFHFSREYIPGFEKPPEGNLLVTRMNAWQDHWKYEDCKGVIWTAGVKTMKELCKKNI
                     WVSGTLDALGEDEHPSLEHILPNPDFIKLTHDRSKEIESVYEKCYTYILKLEEPLPDL
                     RNKTHFFWMSAYQFDLALEHFPEIADKDHSSGPGITRIHIENRLNRKIDVFLSYEEWL
                     EYHST"
BASE COUNT          452 a          264 c          302 g          367 t
ORIGIN      
        1 ctgtttaaga aggaacattt tctcaatcct cctgaaaaac ttgtgattat gtcctcttcg
       61 cccagaagag agtacaattt aaaaaagttt ttttcagagt atcttcctga aaaacttctg
      121 aataaagaaa tcatatttga accagtcaga ggcaatctca gaaccagagc cagaaaatgg
      181 caggatggaa ctgcatcagg aattgtgttc gcaaaggctg gaattgacag aattctgatc
      241 gaacggttca atgaatcagg agatgaagaa ctgaaattag aaagagattt tttccattca
      301 cttctaaatg actgtctttt tctggtgctt cctctatcct ccaatccaaa tgcccctgca
      361 cagggtggaa ttgccgtgga aatcagaaaa ggcagagatg atctgcgcac attcctgaaa
      421 aagatgacga tcaaaaatgt ggacaactgt atcagagggg aaagagaaga actcatgaaa
      481 tacgggggag gatgccatca gaagatcggt gtcagcattc ttcagagaga ctatggaact
      541 gtgatgatcc tgaaaggtga gacagatgac ggtaaaactc tcgactcaaa aaaactcttc
      601 agaattcatt ccccgaaaac atccgatctt tccgggatct ggccgctccg caatgaaggt
      661 tttcattttt ccagagaata tataccaggc tttgaaaagc cccctgaagg caatctgctt
      721 gtgaccagaa tgaatgcctg gcaggaccat tggaaatatg aagactgcaa aggggtcatc
      781 tggactgcag gtgtaaaaac aatgaaggaa ctctgcaaaa aaaatatctg ggtctccggt
      841 acattagatg cccttgggga agatgagcat ccttcacttg aacacatcct gccaaacccc
      901 gattttatta aactgactca tgacaggagc aaggaaatag aatctgttta tgagaaatgt
      961 tatacataca ttttaaaact ggaagaacct ttgcctgatt tacggaataa gactcatttt
     1021 ttctggatga gtgcttatca gtttgatctg gctctggaac attttcccga aattgcggac
     1081 aaggatcatt catccggacc aggaattact cgaattcaca tcgaaaacag actgaacagg
     1141 aaaattgatg tttttctaag ttatgaagaa tggctggaat atcacagcac ctgagtcttt
     1201 ttctgagaca ggctataaat tttttattga cttctaaaca gtgttaaata gttaaggtag
     1261 ttactgattg agaattttct cagtcgaatt gaaaatttcc gggaggaaaa aaatgaaaaa
     1321 atttatcggc ggggccgtac tggctcttct tatcggagga tcactccagg cacagactca
     1381 aggcg
//
LOCUS       DAOQGJ010001343         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74548_length_1385_cov_2.312782, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001343 DAOQGJ010000000
VERSION     DAOQGJ010001343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74548_length_1385_cov_2.312782"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..>1385
                     /locus_tag="PK683_17705"
     CDS             73..>1385
                     /locus_tag="PK683_17705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH10340.1"
                     /translation="MKIDKIKDEFLSNTSHELRTPVHGIIGISESLIDGAAGRLGEET
                     IYNLQMIVSSGKRLSNLINDILDFSKLKNQEIELQSRPTDIRQLFEVVISVINSTIKR
                     EGILLYNEIPVSIPLVYGDENRIQQIIYNLIGNAVKFTYSGFIKVKAVEREEFIEICV
                     EDTGIGIPEDKFEIIFQSFEQADSSIARSYGGTGIGLSITRKLIELHGGKIWLTSETG
                     AGSKFYFTLPKSEHQERDTHEEIQSAHTVEQISLSLTVNENNAESAKPSIMDGENDRN
                     SSIRILVVDDEKINVQILTNHLSLHNYIVDTASDGIEALEKIRNKDYDLVLLDIMMPK
                     MSGYEVCNTLRLENSLYDLPVIMLTAKNQPQDIVVGFDVGANDYLIKPFEKSELLARV
                     KTLVSLKMSVRNAITNAKLANTDALTGLNNRRFLFEQANKEFEIA"
BASE COUNT          496 a          227 c          293 g          369 t
ORIGIN      
        1 tattacagga aaaattttat tcacatcaat aatatagaac tgaaaagaga aaaacagata
       61 aatgaaaggc tgatgaagat agataaaatc aaggatgaat ttttatcgaa tacctctcat
      121 gaattaagaa caccggttca cggaataata ggcatcagcg aatcattgat tgacggtgct
      181 gctggcagac ttggggaaga aacgatatat aatctgcaga tgattgtatc aagcggcaaa
      241 cgcctgtcca atttaattaa tgatattctt gatttttcca aactgaaaaa tcaggaaatc
      301 gaactgcaga gcagacccac agacatacga caattatttg aagttgtaat ttcggtgatt
      361 aattcaacaa ttaagcggga agggatttta ttatataatg aaattcccgt ttcaattcct
      421 cttgtttacg gagatgaaaa tcgaattcag caaattatct acaatctgat aggaaatgcg
      481 gtaaaattca catactcggg tttcataaaa gtaaaggccg tcgaaagaga agagtttatt
      541 gaaatctgcg tagaagatac cgggatagga atacccgaag acaagtttga aataattttc
      601 caatcctttg aacaggcaga cagttccatt gcccgcagtt acggaggaac aggaattgga
      661 ctcagtataa ctagaaaatt aattgaactg cacggaggaa aaatttggct tacatcggaa
      721 acaggcgcgg gatccaaatt ttactttact cttccaaaaa gtgagcacca ggaaagagat
      781 actcacgaag aaattcaaag tgcacataca gttgaacaga tatctctcag tctgactgtc
      841 aatgagaata atgctgaatc cgcaaagcct tcaataatgg atggagaaaa tgacagaaat
      901 tccagcatta gaattctagt tgtcgatgat gaaaaaataa atgttcagat tctgaccaat
      961 catctgtctt tgcataatta tattgtagat actgcatcgg atgggataga agcattggaa
     1021 aaaatcagga acaaggatta tgacttggtt cttctggata taatgatgcc caaaatgtca
     1081 ggctatgaag tatgcaatac tctgcgtctg gaaaattctc tttatgattt gccggttatt
     1141 atgctgacag ccaaaaatca gcctcaggat attgtagtag ggtttgatgt gggagcaaat
     1201 gactatctga tcaaaccatt tgagaagagt gaactgctgg cacgcgtgaa aacattggtc
     1261 tctttgaaaa tgtcggtcag aaatgcaatc acgaatgcaa aacttgcaaa tacggatgcg
     1321 ctgactggtc tgaacaatcg gaggttcctg tttgaacagg caaacaaaga gtttgaaatt
     1381 gcaag
//
LOCUS       DAOQGJ010001344         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74569_length_1385_cov_1.934586, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001344 DAOQGJ010000000
VERSION     DAOQGJ010001344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74569_length_1385_cov_1.934586"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1385
                     /locus_tag="PK683_17710"
     CDS             <1..>1385
                     /locus_tag="PK683_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10341.1"
                     /translation="VLLFYNGSKWGLLLPVSVFMTVSSGWPLQVAAMGLVSGLFFFFL
                     LIQKCRLADTVRFSFLILIGLLLSFPALMPFWEYGKTAARSDWNVSVSQWTICPWYSF
                     QFSNPFFEGRFMWWDGKYRIIKTPMLYISLFFIPSVLVYLKSIYKYGLSFQFKGFHRR
                     YLLFFMFTLIFLLVLASLAPNTNSLRYMFRFLPPGVLIFSVWTGIYLSQKRNVLDKLY
                     LFAGILSNLYAFQFLVSGKIRDSLILIALGIICILLFKFWKFRRTSNFFLSLIQILIF
                     LLSAPFFVSPDTFIHTPAIKKKQLGLNFHGSSVFIREYYADFKNSYLGNAALLYGIKS
                     LNGYSPMVDRKIFEYMCMDYFSRMKCKDYSFLYEKDAATGKTGADLFDLNFIQILGKL
                     EILESYLGRSGWKMETIEGAEKRYTRNRDQSLPDGISNLPKGLDLKDVKVGENAISLS
                     YRTLPEYTAQP"
BASE COUNT          366 a          252 c          261 g          506 t
ORIGIN      
        1 cagttctgct tttttacaac ggctcaaaat ggggactgct tctgcccgtc tctgttttta
       61 tgactgtttc ctccggttgg cctcttcaag ttgcggcaat ggggctggtt tcaggactgt
      121 ttttcttttt tcttctaatt cagaaatgca ggctggcgga taccgtacgt ttttccttcc
      181 ttatcctgat tggtctcctg ctttcttttc ctgccttgat gccgttctgg gaatatggaa
      241 aaacagctgc tcgatctgac tggaatgttt ctgtaagtca gtggacaata tgtccttggt
      301 attcttttca gttttcaaac ccattttttg aaggaagatt tatgtggtgg gacggaaaat
      361 acagaataat taagacacct atgctttata tcagtctgtt ctttattcca tcagttttgg
      421 tatacctcaa gtccatatac aaatatggat tgtcctttca gttcaaaggc tttcatagga
      481 gataccttct tttctttatg ttcacactga ttttcctgct tgttctggca tcactcgctc
      541 ctaatacaaa ttcattaaga tatatgtttc gtttccttcc tcctggggtt ttgatttttt
      601 ctgtctggac aggaatttat ctttcacaaa aaagaaatgt tcttgataaa ttatatcttt
      661 ttgcgggcat actcagtaat ctttatgctt ttcagttctt agtatccgga aaaataagag
      721 attccctaat cctcatagcg ttaggtatca tatgcattct gcttttcaaa ttttggaaat
      781 ttcggagaac ttcaaatttt tttctttctt tgattcagat ccttattttt cttctttcag
      841 ctcctttttt tgtctcaccg gatacattta ttcatactcc ggcgattaaa aaaaaacaac
      901 tgggcttgaa ttttcatggc tcttctgttt ttataagaga gtattatgca gattttaaga
      961 attcctatct tggaaatgca gcacttcttt acggaataaa atcattgaac gggtattctc
     1021 cgatggtaga cagaaaaata tttgaatata tgtgtatgga ttatttttcc agaatgaaat
     1081 gtaaagatta ttcattcctt tatgaaaagg atgctgcaac aggaaaaaca ggagcagatc
     1141 tgtttgatct gaattttatt cagattctgg gcaagcttga aatactggaa tcttatcttg
     1201 gacgttctgg ctggaaaatg gaaacaattg agggggctga aaaaagatat actcggaaca
     1261 gagatcagtc cttgccggac ggaatttcaa atctgcctaa aggactggat ttaaaagatg
     1321 tgaaagtcgg ggaaaatgca atttcattat cttacagaac attgcctgaa tatacggctc
     1381 agcct
//
LOCUS       DAOQGJ010001345         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74638_length_1384_cov_2.633559, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001345 DAOQGJ010000000
VERSION     DAOQGJ010001345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74638_length_1384_cov_2.633559"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PK683_17715"
     CDS             <1..599
                     /locus_tag="PK683_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10342.1"
                     /translation="DKECHIAKWNQLVYKNRNSGEIFTVRDTNNIHLKSERIVQSDFP
                     FKGVVVTGSVCCWCCCPCNNTFTYFLGRDRPYFVKWNIVDSNGKAECGGGDENGPFYM
                     NFETELRTGDSETVYARIKGPPCRFDGSYSQSASKDLHLLVRFRNGKFSYERKVTLKD
                     QIPEEFKEVFEKTVKLKSPSRKQIDWFKVLEEYNGVKY"
     gene            926..>1384
                     /locus_tag="PK683_17720"
     CDS             926..>1384
                     /locus_tag="PK683_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10343.1"
                     /translation="MSSSPAAKSGHISVSADALKSDPLKLDGEWIFFWKKFIPASENL
                     EGQKEVYLKVPASWNEFQTEEGKAGGMGYGTYILYLHFPKESVGKIFTLKIPHMGTAY
                     RMWVGETLVSESGKVGTSYSEMRPAYITRTKSFVLEKETVRITVHVSNFHH"
BASE COUNT          429 a          252 c          331 g          372 t
ORIGIN      
        1 aagacaagga atgtcatatt gcaaaatgga atcagcttgt ttataaaaac aggaattccg
       61 gcgaaatttt tactgtcaga gacacaaata atatacatct gaaatctgaa agaattgtgc
      121 agtcagactt tccgtttaaa ggagtcgtgg tgaccggttc tgtctgctgc tggtgctgct
      181 gcccctgtaa taatacgttc acttattttc taggcaggga caggccgtat tttgtcaaat
      241 ggaacattgt cgattccaat ggaaaggcag aatgcggagg gggggatgaa aatggacctt
      301 tctatatgaa ttttgagacg gaactcagga ctggagattc ggaaaccgtt tatgccagaa
      361 tcaagggtcc accctgcaga tttgacggaa gctattcaca atcagcttca aaagatctgc
      421 atctgcttgt gcgcttcagg aatgggaaat tcagttatga aagaaaggtc actctgaaag
      481 atcagatccc tgaagagttc aaggaggtat ttgaaaagac tgtaaaactg aagagtcctt
      541 ccagaaagca gatcgactgg ttcaaagtcc tcgaggaata caacggagtg aagtactgaa
      601 aaaattcaga ccatgcattt ctgtaaattg actgacacgc cctgcagaaa agatataatg
      661 catggaacct gtcttttttg cgaagcactg agtgaatacg tcgatgggtg atatcagaga
      721 ctgatctgaa gggaagatct ggaccgaagg ttttcccgaa atcagaatag aatcattcgg
      781 atgaaactgc tttttcaaat tgcaaagacg gcatcagcag aatatatggt aatttcggaa
      841 aggaatttta tagagcagta tgtttatgaa atatatattt ttacttattt tttcagtttc
      901 ggcatgttcg ggctggaaga cctcaatgtc ctccagtcct gctgcaaaat caggacatat
      961 ttctgtttct gcggatgcac tgaagagcga ccctctgaaa ctggacggag aatggatttt
     1021 tttctggaaa aagttcattc ctgcttctga aaatctggaa ggacagaagg aggtatatct
     1081 gaaagttcct gcctcctgga atgagtttca gacagaagaa ggcaaggcgg gaggaatggg
     1141 atacggaaca tatattctgt atctgcattt tccgaaggaa agcgtcggga aaatattcac
     1201 tctgaaaatt cctcatatgg gaactgcata cagaatgtgg gttggggaga cccttgtttc
     1261 cgaaagcgga aaagtcggga catcctattc cgagatgcgt cctgcctata tcacccgtac
     1321 aaaaagtttt gttctggaaa aggaaacagt cagaatcaca gtccatgtct ccaattttca
     1381 tcat
//
LOCUS       DAOQGJ010001346         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74811_length_1382_cov_3.674454, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001346 DAOQGJ010000000
VERSION     DAOQGJ010001346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74811_length_1382_cov_3.674454"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PK683_17725"
     CDS             complement(<1..1332)
                     /locus_tag="PK683_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10344.1"
                     /translation="MKKILTSLTALILLVSCSAKKNETSADDALVFLAFQPLTFQISA
                     NITNGAAVSNSMVYFSKSTATSGKNAKADTSTSTSTSTAVSASSEPVFATSAQTDSSG
                     NITFTLAVGEYTAIITGLDNKSLAFKIKIETSEAGSTNKEGKATITYADGSVKVVILK
                     ITNSMTQPSKAITYVCGYNPKGDTAPPELISVDVDSKTLDLSSGSGKVKIKAKLAEGD
                     EGTDSAKKATGIKKVTAKLFSPKRLSGGGYSAYATLTLNSTSGLYEGDATLTDFVENG
                     VWKVGLISARDNAGNERDYIVDKSKSETNYSINGCGQKIDTKIPAPEVTVSGSKPDTE
                     APVLNVGTLTIISEITTASPTPVTNPATIDITTSTASPKETKITVTVTATDAGGTGNA
                     SGVSYIDARLQSYSWWNSSSNYLGNALYIRLDRTSGTNTNGTYSGSAMIRSY"
BASE COUNT          310 a          296 c          315 g          461 t
ORIGIN      
        1 ataggaacgt atcattgcag atccggagta ggtgccgttg gtattggttc cggacgttct
       61 gtccagacgg atatatagtg catttccaag atagttgctg ctggaattcc accaggaata
      121 actctgaagt cttgcatcaa tgtagcttac accgcttgca tttcctgttc cgcctgcatc
      181 tgtagcggtt accgtcacgg taatcttggt ttctttcggt gaagcagttg atgttgttat
      241 gtcgattgtt gctggatttg taaccggtgt cggtgaggca gtggttattt ccgatataat
      301 agtcaatgtt cctacattga gtacaggtgc ttctgtgtca ggtttggatc cgcttacagt
      361 gacttcagga gcagggatct tcgtgtcaat tttctgaccg cagccgttga tggagtaatt
      421 tgtttcactc ttgctcttgt ctacaatgta atctctttca ttgcctgcat tgtctcttgc
      481 agaaatcaga cctactttcc atacaccgtt ttccacgaaa tccgtcagag tcgcatcccc
      541 ttcatacagt ccggaagtgc tgttcagagt cagagtggcg tatgcactgt accctccgcc
      601 ggaaagacgt tttgggctga agagttttgc agtaactttc ttgattccgg ttgctttttt
      661 ggcagaatca gttccttcat caccttcagc aagcttggct ttgattttga cctttcctga
      721 tcctgaactg agatccagtg tcttcgaatc cacatccaca ctgatcagtt ctggaggtgc
      781 agtgtcccct ttcggattgt atccgcagac ataagtgatt gctttggaag gctgcgtcat
      841 actgtttgta attttcagaa tgacgacttt tactgaaccg tctgcgtatg tgattgttgc
      901 ctttccttcc ttgtttgtgc ttccggcctc actggtttca attttaattt tgaaggccag
      961 actcttatta tctagccctg tgataatagc agtgtactcg cctactgcca gagtaaatgt
     1021 aatatttccg gaactgtccg tctgtgcgga agtggcaaaa accggttcag agcttgcaga
     1081 aactgctgtt gaggttgatg tggacgtaga tgtatctgcc tttgcatttt ttcctgaagt
     1141 cgcagtcgac ttggaaaaat agaccattga attggatact gcagcaccgt ttgtaatgtt
     1201 tgcgctgatc tggaatgtca aaggctgaaa ggccaggaaa accagtgcat catctgcaga
     1261 agtctcattt ttttttgcac tgcaggatac taacaggatc aatgcagtca aactagtcag
     1321 gatttttttc ataaaacttt ctttgtttag ccgtatcagc tttaagtttc taaattaaaa
     1381 tt
//
LOCUS       DAOQGJ010001347         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_74937_length_1381_cov_2.676471, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001347 DAOQGJ010000000
VERSION     DAOQGJ010001347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74937_length_1381_cov_2.676471"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK683_17730"
     CDS             <1..189
                     /locus_tag="PK683_17730"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HNH10345.1"
                     /translation="EYVATEAPFRSFGKLGKPVHQLGFGYQKYFESGILTAGFAEDII
                     NFAASPDIGIFASFEGKY"
     gene            236..985
                     /locus_tag="PK683_17735"
     CDS             236..985
                     /locus_tag="PK683_17735"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNH10346.1"
                     /translation="MKKILHSDIERLLVFFLFSFFIIFCSEKTREAKRTQLFPELREK
                     YPIWGIDVSHYQGKIDWKKTKGSGLRFAYIKATEGTDFVDSRFERNWKDAKRLGVPRG
                     AYHFYRLCKDAKKQAANFIRTVPAEFSSLPPVIDLELHKNCSKPPNRDAFLRDLKKFS
                     KLLEKEYEKKPILYVIWDFYEKYLIGEVNDFSLWISDPWGHEEPVLPEVRKWTLWQYD
                     YKGRLKGISGDVDLNFFNGDEEQFQKFLHSR"
     gene            982..>1381
                     /locus_tag="PK683_17740"
     CDS             982..>1381
                     /locus_tag="PK683_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10347.1"
                     /translation="MKRLAFISPIFHPQAFGGSERLALEIATLLSKEFEIHIYTSCAR
                     NYLSWRNEFSAGTEMFGSMFIHRFPTEEERDIGKFNSYYSKILRKFPNVSEKEYSKWL
                     ELQGPNTPSLISRLTAAEENFDCAVFFSYLY"
BASE COUNT          418 a          274 c          305 g          384 t
ORIGIN      
        1 gagtatgtag ccactgaagc accgttcaga agtttcggaa agctaggcaa gccggtgcat
       61 cagcttgggt tcggttatca aaaatacttt gaatccggaa tattgacggc aggatttgct
      121 gaggacataa tcaactttgc ggcatcgccc gatatcggaa ttttcgcttc cttcgaagga
      181 aaatactgat tggcaggcgg tgtcggcagt aaggctgcaa tttggatatt ttataatgaa
      241 aaaaattcta cactctgata ttgaaagact tcttgtattt ttcttatttt ctttttttat
      301 catattctgt tcagagaaaa cccgtgaagc caagagaacc cagctttttc ccgaattgag
      361 agaaaaatat ccaatctggg gaatcgatgt atcacattac caagggaaga tagactggaa
      421 aaaaaccaag ggctccggtc ttcgatttgc ctatataaaa gcgacagaag gaactgattt
      481 tgtggacagc cgatttgagc gaaactggaa agacgctaaa agacttggcg ttcctcgagg
      541 tgcttatcat ttctacaggc tctgcaagga tgctaaaaag caggcagcaa attttatcag
      601 gactgttcct gccgagtttt catcactgcc tccagtgatt gatctggaac tccataaaaa
      661 ctgctcaaag cctccaaacc gagatgcgtt tcttagagac ttgaaaaaat tttcaaaact
      721 gcttgaaaag gaatatgaga aaaaacctat tctctacgta atctgggatt tttacgaaaa
      781 gtatctgatt ggagaggtga atgatttttc gctttggatc agcgacccct ggggtcacga
      841 agagcccgtg ctccctgagg tcagaaaatg gactctctgg cagtacgatt ataagggcag
      901 actgaagggg atttcgggag atgttgattt gaattttttt aacggagacg aagaacagtt
      961 tcagaaattt cttcacagca gatgaaaaga cttgcattca tatcacccat atttcaccct
     1021 caggccttcg gaggatcaga gagacttgca cttgagatag ccacccttct ttcaaaggaa
     1081 tttgaaattc atatctatac cagctgtgca agaaactacc tcagctggag gaatgaattc
     1141 tctgctggaa cggaaatgtt cggatctatg ttcattcatc gatttccgac agaagaagaa
     1201 agagacatcg gaaagttcaa ttcctattat tcaaaaatac tgagaaagtt ccctaatgta
     1261 tctgagaagg agtattctaa atggctcgaa cttcagggac cgaatactcc ctcactgatc
     1321 agccgtctga cagcagccga ggaaaatttt gactgcgcag ttttcttttc ctatctgtat
     1381 t
//
LOCUS       DAOQGJ010001348         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_75147_length_1379_cov_3.030211, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001348 DAOQGJ010000000
VERSION     DAOQGJ010001348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75147_length_1379_cov_3.030211"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PK683_17745"
     CDS             <1..379
                     /locus_tag="PK683_17745"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNH10348.1"
                     /translation="MSYIKTAAIVQGREVKVSKHTFYSGKNKSESYSYIPIIKYTYYV
                     SGNMHTAASSVLKRTDSAGSRKDAEKILENYPDRKEIECYYDPDSPERSVLNTEIPLG
                     SFLGLLFMGAAFFIPGLIFVFVI"
     gene            376..783
                     /locus_tag="PK683_17750"
     CDS             376..783
                     /locus_tag="PK683_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10349.1"
                     /translation="MSYMHFIFWKEKTGDPVIQIGEILKSMYGRNSKCQADLILSEDR
                     INLRIKDYMFTYSLIAENWVQEESKDIADTFGRKRNDYMIIKESRSRVEFYGDDDPDM
                     DYFNDYLLMLERIQRELPVIIFDTENGNFFDEI"
     gene            1056..1367
                     /locus_tag="PK683_17755"
     CDS             1056..1367
                     /locus_tag="PK683_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10350.1"
                     /translation="MNEDTQCEIFTKKNNYSKGECKIISLKDHIMYYKELKCTGLENK
                     TFYKNVRKKGTVRKIDGIEIYTIGNRKGNLTDDAFIRSAPTTSAKPHSCKFEQYGMDK
                     V"
BASE COUNT          480 a          198 c          287 g          414 t
ORIGIN      
        1 gatgtcctat ataaagactg cggctattgt gcagggaaga gaagtaaaag taagtaagca
       61 tacattctat tcaggaaaga ataaaagtga gagctattca tatataccta taataaaata
      121 tacgtattat gtttcaggga atatgcacac tgcagcatct tcggttctga aaagaacgga
      181 cagtgcgggc agcagaaagg atgcagaaaa aattctggaa aactatcctg acagaaaaga
      241 gattgaatgt tattatgacc ctgattcgcc ggaaaggtct gttctgaata cagaaattcc
      301 gcttggatcg tttttgggat tactttttat gggagcagcc ttttttattc cggggctgat
      361 atttgtgttt gtgatatgag ttatatgcat tttattttct ggaaagaaaa gacgggagat
      421 ccagtcatac agatcggcga gattctgaaa tcgatgtacg gcaggaattc gaaatgccaa
      481 gcggatttga ttctttctga ggacagaata aaccttagaa taaaagacta tatgtttact
      541 tattctttga ttgcggaaaa ctgggtgcag gaagaatcca aagatatcgc tgatacgttc
      601 ggaagaaaac gaaatgatta tatgatcatt aaagagtccc ggagcagagt tgaattttac
      661 ggggatgatg atcctgatat ggattatttc aatgattatc ttctgatgct ggagagaatt
      721 caaagagaac tgccagtgat tatattcgat acggaaaatg gaaatttttt cgatgagata
      781 tgattgcatt ataatatttc tgtttacaaa tctcttgtcg tctttatttt tacagctatg
      841 aaaaaattca aaattatatt ctcagcagct ttgtttgcca tgactcgcgg gatttctgca
      901 aatgaatttt ccggtgaatg gcactcacaa gaggataact tagcttatat aataagtatt
      961 tctgatactt cgattggaat ataatattca gagaaggctg tcctttaaat ctggaaggca
     1021 cttataaaat aagaggaaaa actctttttt atgaaatgaa tgaggataca caatgtgaga
     1081 tatttacaaa aaaaaataac tattcaaaag gtgaatgtaa aatcatttca ttgaaagatc
     1141 atataatgta ttataaagaa ctgaaatgca ctggtctaga aaataaaaca ttttacaaga
     1201 atgtacgcaa aaaaggaacc gtccgcaaaa tcgacgggat agaaatctat acgataggaa
     1261 atcggaaggg aaatcttact gatgatgctt ttattcgatc tgctccgact acaagtgcaa
     1321 aacctcattc ctgtaagttt gagcagtatg gaatggataa agtctaaaat gttctccct
//
LOCUS       DAOQGJ010001349         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_75158_length_1379_cov_2.817976, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001349 DAOQGJ010000000
VERSION     DAOQGJ010001349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75158_length_1379_cov_2.817976"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1379)
                     /locus_tag="PK683_17760"
     CDS             complement(<1..>1379)
                     /locus_tag="PK683_17760"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNH10351.1"
                     /translation="NELEILERRGKINPIWKNRAEILRLGISGSAKKDGLTFFRHISG
                     DDHRGFRFKNPTDALVDTEGNLYILSYGTSNIVKFDPNGLPVGNYKGGFGRTFDKPLF
                     FVLHKDTLYVSDFGADMIYKISEKGRFLERFGEHGLGAGQFHGPSGLDVSGSNYLYVS
                     DSGNNRIQKFSLDGKFIQEFGKSSAPGQTKLNFPAGLCIGEKGEIYVADKGNRRIAVF
                     DEEGNFLKEFTHPNMKMPRSVKFFKGRMYVSDELFGLLVYNPATEKWTKISSYRDSSG
                     DYVKMLRPFSSTADYTGSLYTVDYSKHKINVFSPRNSLTSNLNVFVERAELANFPTIS
                     FFVRIKNRNNQDLGGIRREAFRVTENSNVHPLVGLANMKEFNDKISVSLVFENSPKVH
                     EVTDNLNSFLGNFFSSFTKKDRIEVIRAGKDSEKVYDFGRSPLDVYARIRKSTPEKNN
                     INLGKSLYQ"
BASE COUNT          379 a          307 c          262 g          431 t
ORIGIN      
        1 cctgatacag actttttcca agattgatat tgtttttttc gggtgtagat tttctgatcc
       61 ttgcataaac atccagaggg gatctgccga agtcatatac tttttctgaa tcctttcctg
      121 cacggatcac ttctatcctg tccttctttg tgaaggaaga aaagaaattt ccgagaaaac
      181 tgttcagatt gtctgtcact tcgtgaacct ttggactgtt ttcaaacacg agagagacgg
      241 atattttatc attaaattct ttcatatttg cgagaccgac gagaggatga acatttgaat
      301 tctccgtgac tcggaatgcc tctcttctga tccctcccag atcctgattg ttcctgttct
      361 taattcttac aaaaaaagaa attgtaggaa aatttgccag ttcagctctt tccacaaaaa
      421 cattcaaatt ggatgtaagc gaatttcgag gtgaaaatac attaatcttg tgcttggaat
      481 agtcaactgt gtacagagaa cctgtatagt ccgcagtgct cgaaaaagga cggagcattt
      541 ttacatagtc gcctgaactg tctctgtagc tggaaatctt tgtccatttt tctgtggcag
      601 gattatatac aagaagtccg aaaagttcat cagagacata catccttccc ttgaaaaatt
      661 tcacggacct gggcattttc atattcggat gagtaaattc tttgagaaaa tttccctctt
      721 catcaaagac cgcgatcctt ctgtttccct tgtcggccac atagatttca cctttttcac
      781 ctatgcatag tcctgcagga aaattgagtt ttgtctgacc tggagcagaa gactttccaa
      841 attcctgtat gaattttcca tccagactga atttctgaat cctgttgttt ccagaatcgg
      901 aaacataaag ataattggaa ccgctgacat cgagtcctga gggtccgtga aactgtcctg
      961 ccccaagtcc atgttcgccg aatctttcga ggaatctccc cttttcagag attttgtaaa
     1021 tcatatccgc accgaaatca ctgacataca gtgtatcctt gtgcagtaca aagaaaagag
     1081 gcttgtcaaa ggttctcccg aatccgccct tgtaatttcc gacaggaagt ccgttaggat
     1141 caaattttac aatattgctt gttccgtagg aaagaatgta cagatttccc tcagtgtcca
     1201 caagtgcatc agtcggattc ttaaaccgga aaccgcgatg atcatcacct gagatatgac
     1261 ggaaaaaagt aagtccgtcc tttttggcac ttcctgaaat tccaagacgg agaatctctg
     1321 cacgattctt ccagattgga ttgatcttcc ctcttctttc caggatctcc agttcattc
//
LOCUS       DAOQGJ010001350         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_75203_length_1379_cov_1.999245, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001350 DAOQGJ010000000
VERSION     DAOQGJ010001350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75203_length_1379_cov_1.999245"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1379)
                     /locus_tag="PK683_17765"
     CDS             complement(<1..>1379)
                     /locus_tag="PK683_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10352.1"
                     /translation="NSATQAGETALMWSVYNGERSIFDLLIKNGAKLAVKSRSNLTVM
                     GIAELIEDTNAIIAEQKTEPKQDPLPAAFSQKELIEKYKKEIECNFLAKPWEYIQNML
                     GKKTCISLAGTMDDRIKKLSSDNKEARQISKELTEYAKPAPDPTVQLERASLIPEPAE
                     MNARKLDYDRNMSVYDRKRRRNEMAEFFNRENDPGQSSAAKDRFQECDLPGKPDLKPI
                     SVEVKDKIQFEANLKNKAVPETQDPDMSLWLKDIAEKKHERVLISNMEDWNKFLLIKR
                     KLRRNLEDVLNAYAEFASFRDFAASKPSESEYQEMAELSGRVAKLKAETLQKRLQATP
                     KCNVRDFQKYNARLKDASDLISKGLDLLKKGKSYNNILEKIVSDESAKKPIDTDCSTL
                     ASDPDLEKKFGTRRQAIGEAMTRCYKNSLELKSPNDQYLRLAQTYGNQSIIEYLDTVR
                     TAYNNKESF"
BASE COUNT          291 a          303 c          272 g          513 t
ORIGIN      
        1 gaaacgattc tttattatta tatgcagttc ttaccgtgtc cagatattca atgatgcttt
       61 ggtttccgta agtctgagcc agtctgagat actgatcatt cggagatttc agctcaagtg
      121 agtttttata gcatctcgtc attgcctctc caatagcttg gcgtcttgta ccgaattttt
      181 tttcaaggtc agggtcagat gcaagggtgc tgcagtcagt atcaataggc tttttggcac
      241 tttcgtctga gacgattttt tccagaatat tattgtagga ctttcccttt ttgagtaaat
      301 ccagaccctt ggaaataaga tcgcttgcat ctttcagtct tgcgttatat ttctgaaagt
      361 cccttacatt gcattttgga gtggcctgca gtcttttctg cagggtctca gccttcagtt
      421 ttgctaccct tccggacagc tcggccattt cctggtattc cgattcagat ggtttggatg
      481 ctgcaaaatc tctgaaggat gcaaattctg cataggcatt cagaacatct tccaggtttc
      541 ttctcagttt tcttttaata agcagaaact tattccagtc ttccatattg gagatcagta
      601 cccgttcatg ttttttttct gcaatatcct taagccataa gctcatatcc ggatcctgcg
      661 tttccggtac tgctttgttt tttaaattcg cttcaaattg tatcttatcc ttaacttcca
      721 cggatattgg tttcaagtct ggctttccgg gaagatcaca ttcctgaaat ctgtcttttg
      781 cggcagacga ctggcctggg tcattttccc tattgaagaa ttcagccatt tcgtttcgtc
      841 ttctctttct gtcgtagaca gacatattcc tgtcatagtc cagttttctt gcattcattt
      901 ctgccggttc cggaattaga cttgctcttt ccagctgcac agtcggatcc ggggcaggtt
      961 ttgcatattc tgtaagttct tttgatatct gacgggcttc tttattgtct gaggacagtt
     1021 tttttattct gtcatccatt gttccggcca gactgataca ggtctttttt ccaagcatat
     1081 tctgaatata ctcccatggc tttgcaagga aattgcattc aatttccttt ttatattttt
     1141 cgataagttc tttctgagag aaagcggctg gaagcggatc ctgtttcggt tcggttttct
     1201 gttctgcaat aattgcattc gtatcttcta taagttctgc aattcccatt acagtcaaat
     1261 tgcttcggga ctttacggcg agttttgctc cgtttttgat cagcagatca aatatggatc
     1321 gttctccatt gtaaactgac cacatcagtg cagtctctcc cgcctgtgtc gctgagttt
//
LOCUS       DAOQGJ010001351         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_75743_length_1373_cov_4.944613, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001351 DAOQGJ010000000
VERSION     DAOQGJ010001351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75743_length_1373_cov_4.944613"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..>1373)
                     /locus_tag="PK683_17770"
     CDS             complement(123..>1373)
                     /locus_tag="PK683_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH10353.1"
                     /translation="GYFRIFDELKTYLCEITGFSAVSLQPNAGSQGEYAGLLTIRNYD
                     ISRGQRNRNICLIPVSAHGTNPASAVMAGYTVVPVACDSDGNVDLNDLRKKCGEHSQN
                     IGVLMITYPSTHGVFEETVREICTLIHSHGAHVYMDGANMNAQVGLCRPADIGADVCH
                     LNLHKTFCIPHGGGGPGVGPIGVRKHLEDFLPGHILYDNGSGNTEGSVTSSPFGSANI
                     LLISYVYIRLMGAAGLKEATMTAILNANYISKKLEKHYPTLYKGKNGLAAHECILDFR
                     QFKSAGVEVEDIAKRLMDYGFHAPTMSWPVPGTLMVEPTESESLYELDRFIEAMIHIR
                     EEIRDAEENRISRTDNPLKNSPHTAEKIASENWTHPYSREKAAYPVKGLRDFKFWPYV
                     GRIDNVYGDRNLFCSCLPMEDYEK"
BASE COUNT          344 a          319 c          303 g          407 t
ORIGIN      
        1 tattgtttta ttaatacgat ttctacttca ggattaagaa tgattcgaat aaataaaaac
       61 atttcctggc aggaccataa tcaatagtaa cttttcagct catgaatgct ataacgatca
      121 ttttattttt cgtagtcttc catcggaagg caggaacaga aaagatttct gtctccgtac
      181 acattgtcga tccgaccgac gtaaggccag aatttgaaat ctctcagtcc ttttaccgga
      241 tatgcagcct tttctctgct gtaaggatgc gtccagtttt cagatgcaat cttttcagca
      301 gtatgtggcg aattcttgag agggttgtct gtcctgctga ttctgttttc ctccgcatcc
      361 ctgatttctt cacggatatg aatcatcgct tcaatgaatc tgtccagttc ataaagagat
      421 tcgctttctg tcggttcgac catcagtgtt cccggaacag gccaggacat tgtaggtgca
      481 tggaatccgt aatccataag cctttttgct atgtcttcca cttccacgcc tgcggacttg
      541 aactgacgga aatcgagaat gcactcatgt gccgcaagac cgttctttcc cttgtagaga
      601 gtaggatagt gcttttccag ttttttggaa atatagtttg cattcaggat tgcagtcatt
      661 gtcgcttcct tcagaccggc tgcgcccatt aaacgtatgt agacatatga aatgagcaga
      721 atgtttgcac ttccgaaagg ggatgatgtc acggatcctt ctgtatttcc gcttccgttg
      781 tcgtaaagaa tatgtcccgg aaggaagtct tccagatgtt ttctgacgcc gatcggaccg
      841 acgccgggac ctcccccccc gtgaggaatg cagaaggtct tgtgcagatt cagatggcag
      901 acgtctgcac ctatgtcagc gggtctgcag agacctacct gagcattcat atttgcgccg
      961 tccatataca catgagcacc gtgagaatga atgagcgtgc agatttccct gacagtctcc
     1021 tcgaatacac cgtgagtgga aggatatgtg atcattaaaa cacctatatt ctgagaatgc
     1081 tcaccgcatt ttttcctgag atcattcaga tccacgtttc catcactgtc gcaggcaaca
     1141 ggaaccactg tgtaacctgc cattacagca ctcgcagggt ttgttccgtg ggctgaaacc
     1201 ggaatcaggc agatgtttct gtttctctga cctctggata tatcataatt acggattgta
     1261 agaagtcctg catactctcc ctgagagcct gcattcggct gaagagagac tgcactgaat
     1321 ccggtgattt cacagagata agttttgagt tcatcaaata ttctgaagta gcc
//
LOCUS       DAOQGJ010001352         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_75779_length_1373_cov_3.154021, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001352 DAOQGJ010000000
VERSION     DAOQGJ010001352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75779_length_1373_cov_3.154021"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PK683_17775"
     CDS             <1..759
                     /locus_tag="PK683_17775"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNH10354.1"
                     /translation="GGALFTFMELGLLKTVLGSSVIRRTDWLTRQYIQNDFRSYSNML
                     GAVLLADFAFTYFRTENWNVEGKFQGEKAPDYYPPTAPLSRLWRSALIPGWGQIYAGN
                     TFKGGFMMTVFFISGFISQIADPSVIKSYKKEMKESDIEYAAFAYPYSDRYSIQQIAD
                     ADITRIWLTDRFGAKSQIDFYRFDQNRFSTVLGAVWVISMIDAYFFSGRNSAESSEKE
                     KSLSFRPEINTEKVKDSGTLKNETAMKGSLIYHW"
     gene            complement(959..>1373)
                     /locus_tag="PK683_17780"
     CDS             complement(959..>1373)
                     /locus_tag="PK683_17780"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNH10355.1"
                     /translation="GLGILISDALPAFMIPDAFLVFAVAGKLDDFPVIFYSCVGSMIG
                     GSISYLLGMYVFPRMKFAKNFIDRHEENLKPYVEKYGVWAVVLAATTPLPYSWMAILV
                     GTLKMPYREFIAASLARIPRFVFYYYAIKLGWVVS"
BASE COUNT          450 a          276 c          306 g          341 t
ORIGIN      
        1 ggaggagcac tgtttacttt tatggaactt ggactgctga aaacagtgct tggatccagc
       61 gtcatacgca gaacagactg gctgacaagg cagtacatac agaatgattt ccgctcatat
      121 tccaatatgc tcggggctgt ccttctggca gattttgcat tcacctattt caggacggaa
      181 aactggaatg tggaaggaaa gtttcaggga gaaaaagcgc ctgattatta tccgccgact
      241 gccccgcttt cgagactctg gagatcagct ctgattccgg gctggggaca gatctatgca
      301 gggaatactt tcaagggagg atttatgatg actgtatttt ttatttcggg attcatatcc
      361 cagattgctg atccctctgt gataaaatcc tataaaaagg aaatgaagga atcagacatt
      421 gaatatgcag cgttcgcata tccatattct gacagatatt cgatacagca gattgccgat
      481 gctgatatca ccaggatttg gctgacagac agatttggtg ctaaatctca gattgacttt
      541 tacaggttcg atcagaacag attcagcaca gtccttggcg ctgtctgggt catcagtatg
      601 attgatgcct attttttcag tggaagaaat tcggcagaaa gttcggaaaa agaaaaatct
      661 ctctccttca gaccggaaat caatacagaa aaagtaaaag attccggaac tctgaaaaat
      721 gaaactgcaa tgaaaggatc attaatttat cactggtgac agccgagtgt ccaaaatatc
      781 aaagaaaatt gaaaaaaaaa tcaatacttt ttttatactg cgaaactgaa aaagacaaca
      841 ctggacacaa caggattttt aggacgaaca ggataaaaat gatggagctg caattcctgt
      901 aaaagcagca gatcgggaaa aagtaagttt gctgttcccg gaggatgaac aaagaagatc
      961 aggaaaccac ccacccgagt ttgatagcat aatagtaaaa tacgaatctg ggaattctgg
     1021 caagactcgc agcaataaac tctctgtaag gcattttcag ggtgcctaca aggattgcca
     1081 tccaggaata aggaagaggc gttgtagcag ccagcacgac cgcccagaca ccgtactttt
     1141 ccacataggg tttcagattt tcttcatgtc tgtctatgaa gtttttagca aacttcatcc
     1201 tgggaaagac atacattccg agcagatagg aaattgaccc gccgatcata cttccgacgc
     1261 aggaataaaa aattacagga aaatcatcca gcttgccggc aactgcaaat acaagaaaag
     1321 catccggaat cataaatgca ggaagggcat cgctgatcag aattccaagt cct
//
LOCUS       DAOQGJ010001353         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_75885_length_1372_cov_2.993166, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001353 DAOQGJ010000000
VERSION     DAOQGJ010001353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75885_length_1372_cov_2.993166"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PK683_17785"
     CDS             <1..587
                     /locus_tag="PK683_17785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH10356.1"
                     /translation="ADSHISREYGGSGLGLAIVRKLLHQMNSTVSVRSRPGEGSVFSF
                     KLLLPKGVSEETGISSTGVKVIVSRFETMKILIVDDNDLNLRLAERMLLKWKKAGVDS
                     AHSGKQALQFVSENNYDLILMDLHMPVMDGYAVTEEIRNMKDFRKRTVPIVALTASVM
                     QEEKLKAEKVGMNGFLVKPLDQKELYEMLVRFLS"
     gene            650..1228
                     /locus_tag="PK683_17790"
     CDS             650..1228
                     /locus_tag="PK683_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10357.1"
                     /translation="MKQLFNLFIITFSILNCVSFKDKENPGGDSGKARNYFSDRIMDS
                     RDILTFTAVYGAAAGVKAQVGPLGAGLYFQPGSVGHGVDLSSETGLKSAEVTDHHVTD
                     FAILLGTDSSQPNSLTDSGLRHEFRNKGYKSPFRHPPAYTRLGFAATLGIGVRVELNI
                     GELLDFLLGFALIDIYGDDIYLKNSAGSAKKQ"
BASE COUNT          401 a          271 c          328 g          372 t
ORIGIN      
        1 aggcagattc tcatatatcg agagaatacg gcggatcagg actcggactg gcaattgtaa
       61 gaaaacttct gcatcagatg aacagcactg tatctgtccg aagcaggcct ggagaagggt
      121 ctgtcttctc tttcaagctt ctccttccta aaggtgtatc cgaggaaaca ggcatcagtt
      181 ccaccggagt caaggtcatt gtatcgcggt ttgaaacgat gaaaattctg atagtggatg
      241 acaatgatct gaacctcagg cttgccgagc ggatgctgct caagtggaaa aaggcgggag
      301 tggacagtgc tcacagcgga aagcaggctc ttcagtttgt atcagaaaat aattatgatt
      361 taattttaat ggatctccat atgccggtaa tggacggata tgcagtaact gaagaaatcc
      421 gcaatatgaa ggatttcaga aaaagaacag tgcctatagt tgcgctgaca gcgtcagtaa
      481 tgcaggaaga aaaactgaag gcagaaaaag tcggaatgaa cggttttctg gtaaaacctc
      541 tcgatcagaa ggaactgtat gagatgcttg tccgttttct ttcctgatgc acttgcagta
      601 aataaaataa aatgatgaca gaagcatatg gctgataaaa agatttccca tgaaacaact
      661 gttcaatctg tttatcatca cattttccat tctaaactgt gtcagtttca aggataagga
      721 aaatccaggc ggagattccg ggaaagccag aaattatttc agtgacagaa ttatggactc
      781 ccgggacata cttactttca ctgcagtcta cggtgctgcc gcaggtgtca aggctcaggt
      841 cggacccctc ggtgcaggac tgtattttca gcctggttca gtcggacatg gtgtagatct
      901 ctccagtgag acaggtctga agtcggcaga agttaccgat caccatgtga ctgattttgc
      961 gattcttctg gggacggact caagtcagcc gaattcactt acagattcag gcctgcgcca
     1021 tgaatttagg aacaaaggtt acaaatcccc tttcaggcat cctcctgcgt atacaagact
     1081 cggttttgcc gctacacttg ggatcggagt ccgggtggaa ttgaatatcg gtgaacttct
     1141 cgactttctc ttaggttttg cactgattga tatttacggg gatgatatct atctgaagaa
     1201 ttctgcagga agtgcaaaaa aacagtagaa cactgtttcg atattctgat tctggaattt
     1261 ctgaaaggga gcgctgagat ttggataaaa tgaggcagaa tcttcctttc taaaaaagga
     1321 ttcactatga aacatcttat tcgtctgttc atcattacag tttccattgt aa
//
LOCUS       DAOQGJ010001354         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76059_length_1370_cov_3.460837, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001354 DAOQGJ010000000
VERSION     DAOQGJ010001354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76059_length_1370_cov_3.460837"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PK683_17795"
     CDS             complement(<1..1116)
                     /locus_tag="PK683_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10358.1"
                     /translation="MSMFDFDANTEGTALILPESSVKRVQRLIASAKHSSSFPKVQSV
                     LEKEFGFFMLGKINIDSNFWLSVDLEEKIINSIQSEIPLYEELFAAGKDDVMREAFSL
                     LPSDDSQINILEFFIRLPILFQSKVRYLICVMETSESLERIEFRISPLEGRTVKWYDL
                     NYFHGMVAGVMALFQMKDWNIAAKETSINYSPNQFPPNLICTKKGIETRFEVYWKESN
                     ILLGKTYLKPDSEITNTPSFVMSKTGDESDNFTYIDLGAMVEKSQKLYYENRDLEAAV
                     EVLNNFRNELMVKQKAISKDLRMARNIQRGIIPQSIPDWKGLQFAFHFQPMQEVSGDY
                     YDYFNFGSNKIGLMLSDVSGHGVPAAFITAISKLLFTN"
     gene            1122..>1370
                     /locus_tag="PK683_17800"
     CDS             1122..>1370
                     /locus_tag="PK683_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10359.1"
                     /translation="MLKYIFKAENYKYFLSLFSVSSAIIIYYILNLFWTTYLTCPNRD
                     FLSWDGDLRFISALGLLDSFRNLNFGDFVFKILDTPTWP"
BASE COUNT          385 a          272 c          266 g          447 t
ORIGIN      
        1 atttgtaaaa agcagtttgg agatcgcggt aatgaatgca gcaggaactc catgtcctga
       61 aacgtccgaa agcatcaggc cgatcttatt tgaaccgaaa ttaaaatagt cgtagtagtc
      121 tccgctgact tcctgcatcg gctgaaaatg aaaagcaaat tgcagacctt tccagtccgg
      181 tatggactga gggataatcc ctctttgaat gtttctggcc atcctgagat ctttggaaat
      241 ggctttctgc ttgaccatca gttcatttct gaaattgttc aggacttcta cggcagcttc
      301 cagatctctg ttttcatagt agagtttctg agacttttct accattgcac ccagatcaat
      361 ataggtgaaa ttgtccgatt catcgcctgt ttttgacatt acgaaactgg gagtatttgt
      421 gatttctgaa tccggtttaa gataggtttt gcctaaaagg atattgctct ctttccaata
      481 gacctcaaat cgggtctcta ttcctttttt ggtacagatg agattcggag gaaactgatt
      541 gggactgtaa ttgatgctgg tttcttttgc agcaatattc cagtctttca tttgaaacag
      601 tgccattacc ccggctacca tcccgtgaaa ataattcagg tcgtaccatt tgaccgtcct
      661 tccttccaaa ggcgaaattc tgaattcgat tctttccaaa ctttcagaag tttccatcac
      721 acagatcaga tagcgcactt ttgactggaa aagaatggga agtctgatga aaaattcgag
      781 aatattgatc tggctgtcat ctgaaggaag gagactgaat gcttctctca taacgtcatc
      841 ttttcccgca gcaaaaagtt cctcgtacaa tggaatttcc gactgaattg agttgataat
      901 tttttcttcc agatcaacgg aaagccagaa attgctgtca atattgattt ttcccagcat
      961 aaaaaaaccg aattcttttt ctaaaacgga ttggactttt ggaaaggatg aactatgttt
     1021 ggcagatgca atcagcctct gaactctttt tacagaggat tcagggagta tgagagcagt
     1081 tccttcagta ttggcatcaa aatcaaacat agacaaaaag tatgctcaag tatatattca
     1141 aagctgagaa ttacaagtat tttttaagtc ttttttcagt ttcctctgca attattattt
     1201 actatatttt aaatcttttt tggactacct atctgacctg tccaaacaga gattttcttt
     1261 cctgggacgg agacctgaga tttatttctg cactaggact tctggattca tttagaaatc
     1321 tgaatttcgg tgattttgta ttcaaaatat tggacacccc cacttggccg
//
LOCUS       DAOQGJ010001355         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76153_length_1369_cov_3.957382, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001355 DAOQGJ010000000
VERSION     DAOQGJ010001355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76153_length_1369_cov_3.957382"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1369
                     /locus_tag="PK683_17805"
     CDS             <1..>1369
                     /locus_tag="PK683_17805"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNH10360.1"
                     /translation="TLTQQTARIVNRDFFPKNIYLRKILEIFYAVRYEIHFTKKEILE
                     IYLNSVPVRSNLEGFPSAAQAIFQKNVSFLSKQESFSLVLLLRNNSQSETAFRKRWIM
                     SSDSADLGIKEVPEDIIRIIYHEKYSIQKQEDRNTDHYEAFLRKIFPNEKGRIVSSLD
                     VNLNTSLRNVIREEMEYMNMHHVTNAAVIVLKLPKDGERFLELKAMVGSRDYFESLEG
                     QVNGTAAYRHAGSTMKPLLYALAFEMNLIGPETILDDSKVFVPIPDGGVYSPKNNDLQ
                     YWGKMTASEALANSRNIPAVKIITMTGVSRFRETLLNAGLSHLVKSPDYYGPGLSLGN
                     GGVSLIQLTRAYTVFSENGILYPILMGKNEEGRPLSFGEEKKVFSRETADRIKYILSE
                     KELRRRAFGERNFFDFPFDAAVKTGTSKDHRDAWAAGFSAEYAVGVWVGNFDGSSMNR
                     ITGGWG"
BASE COUNT          446 a          270 c          305 g          348 t
ORIGIN      
        1 caacactgac tcagcagact gcaaggatcg taaacagaga tttttttcct aaaaatattt
       61 atctgagaaa gattctggaa attttttatg cagtaagata cgaaattcat tttacgaaaa
      121 aggaaatcct tgaaatctat ctgaattcag tgccggtacg ttccaatctg gagggatttc
      181 cttccgctgc ccaggccatt tttcaaaaga atgtctcctt tctctcaaag caggaatcat
      241 tcagccttgt gctccttctc agaaacaatt ctcagtcgga aacggcattc agaaaacgct
      301 ggataatgtc ttctgattcc gcagatctcg ggataaagga agttcctgag gatataatac
      361 gaataatata tcatgaaaaa tattccatac agaaacagga agacagaaat acggatcatt
      421 atgaagcatt tctccggaaa atatttccga atgaaaaagg aagaatcgtt tcatctctgg
      481 atgtaaattt gaatacttct ttaagaaatg tgattcggga agaaatggaa tatatgaata
      541 tgcatcatgt gaccaacgcc gcagtcattg tactgaaact tcccaaagac ggagaaaggt
      601 ttctggaact gaaggcaatg gtcggatcaa gagactattt tgaaagcctg gaaggccagg
      661 tcaacggaac agctgcctac agacatgccg ggagcacaat gaagcctctg ctgtatgcac
      721 ttgcatttga aatgaatctg atcggtcctg aaactatact ggatgattct aaagtatttg
      781 tgccgattcc ggacggaggg gtttacagcc caaagaacaa tgatcttcaa tactggggaa
      841 aaatgaccgc cagcgaagcc cttgcaaatt ccaggaacat tcctgcagta aaaatcatca
      901 caatgacagg tgtctcaagg ttcagagaaa cccttctgaa cgcaggactt tctcatttgg
      961 taaaatcccc cgactattac ggacccggtc tttctctcgg aaacggcgga gtaagtctga
     1021 tacagctgac aagggcatat actgtcttct ctgaaaatgg aatactctat ccgatcctga
     1081 tggggaaaaa tgaggagggc agaccgcttt cattcggaga agaaaaaaaa gttttcagca
     1141 gggagactgc tgacagaata aaatatattc tctcagaaaa agaactcaga agaagagcat
     1201 tcggtgaaag aaacttcttt gattttcctt ttgatgctgc agtcaaaaca ggaacatcca
     1261 aagatcacag ggatgcctgg gccgcaggat tttcagctga atatgcagtc ggagtatggg
     1321 tcggaaattt tgacggttct tcaatgaaca gaatcacagg aggctgggg
//
LOCUS       DAOQGJ010001356         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76170_length_1369_cov_3.262557, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001356 DAOQGJ010000000
VERSION     DAOQGJ010001356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76170_length_1369_cov_3.262557"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..>1369
                     /locus_tag="PK683_17810"
     CDS             143..>1369
                     /locus_tag="PK683_17810"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10361.1"
                     /translation="MPEVFAFIQARSSSERLPEKTILPLDSEGTAILDHIYRRLSQVM
                     DTERVVFLVPEKDEKIISFLKKRNYRFFTGDEHDVRSRYRNASLHFGAEWIIRLTGDN
                     PILDIPHMELLIEMLRMSSEFDIISYHSLPIGMAGEMFSREALMLDADVSLERHREHV
                     TLHIKENPDRFKIIKLHPFIMKKDISGTAEIRVTVDEERDFRMVQEIFRRFSDSESFG
                     ADELLKLYAEDPVFFQINRDVNQVSFSLPMPVLGQEKKKVHIHFGNPAKFGSGHRERC
                     RILHSCLQIKGWDLSVSDDWNGLPESADLYIIDSRDAELPDSLIDKKVLILDGFQELK
                     RDSHQVYYSLPHFSYPLEKIRNRILFSSLLESDFNEQEIQGRILVYAGNIPELQCRML
                     DRKLYESSLFSEIVRIG"
BASE COUNT          400 a          253 c          316 g          400 t
ORIGIN      
        1 aaaatcatta tcgggaatct cgaaaccgat tcagtttcag aaatttggca gaaaggggag
       61 gagagttttc gattcagtct gaacggagaa tatgaaaaac tgatgtcccc ctgcactgtc
      121 tgtgatgaat ggtacacctt caatgcctga ggtatttgca tttatacagg caaggagttc
      181 ttcagagaga cttcctgaaa aaacgattct tccgctcgat tcagaaggca ctgctattct
      241 ggatcatata tacagaagac tgtctcaggt aatggataca gagagggttg tttttcttgt
      301 tcctgaaaag gatgaaaaaa tcatctcgtt tctgaaaaaa aggaattacc gcttttttac
      361 cggcgatgaa catgatgtgc gatccagata cagaaatgcc tctcttcatt tcggtgctga
      421 atggatcatc agactcactg gcgacaatcc gatcctggat attcctcata tggagctgct
      481 tatcgaaatg ctgagaatgt cgtcagagtt tgacattatt tcctatcatt ctctcccgat
      541 tgggatggcg ggtgaaatgt tcagccggga ggcactgatg ctggacgcag atgtttcact
      601 ggaaagacac agggagcatg tcactcttca tataaaagaa aaccctgacc ggtttaaaat
      661 cattaaattg catccgttta tcatgaaaaa agacatttct ggaactgcag agatcagagt
      721 gacggtcgat gaagaaaggg atttcagaat ggttcaggaa attttccgca gattttcgga
      781 cagtgaaagt ttcggtgcgg atgagcttct gaagctgtat gctgaagatc cggttttttt
      841 tcagatcaac agggatgtga atcaagtcag cttttctctc cctatgcctg ttctcggtca
      901 ggagaaaaaa aaggtgcaca ttcattttgg aaatcctgct aagttcggtt caggtcacag
      961 ggagagatgc aggatacttc actcctgtct gcagataaaa ggctgggatc tgagtgtatc
     1021 cgatgactgg aacggtcttc cggaatctgc tgatctttat ataatagatt caagggatgc
     1081 ggaacttcct gattccctta tagataaaaa agttcttata ctggacggtt ttcaggagct
     1141 taagagagat tctcatcagg tttattattc cctgcctcat ttttcctatc ctttggaaaa
     1201 gataaggaac aggatacttt tttcatctct tcttgaatca gattttaatg aacaagaaat
     1261 acagggaaga atacttgttt atgctggaaa tatcccggaa cttcagtgca ggatgcttga
     1321 cagaaaactc tatgaaagca gcttgttttc tgaaattgta aggatagga
//
LOCUS       DAOQGJ010001357         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76450_length_1366_cov_4.089245, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001357 DAOQGJ010000000
VERSION     DAOQGJ010001357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76450_length_1366_cov_4.089245"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PK683_17815"
     CDS             complement(<1..432)
                     /locus_tag="PK683_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNH10362.1"
                     /translation="MSLVIESDFTHLHLHTIYSTLDGAIRIPDLMKHVKASGMSSVGI
                     SDHGNMFGAIEFYKEALKQGIKPIIGSEFYVSANRSEEAELENIPDGNAYHLVLLAKN
                     EVGYKNLIKLSSRSFTEGFYRKPRIDYDLLSRNCEGVIALTA"
     gene            535..837
                     /locus_tag="PK683_17820"
     CDS             535..837
                     /locus_tag="PK683_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10363.1"
                     /translation="MPAILTEVSKSYFDRLIQMQMHPKDAVRYGLILGIYESEFEETD
                     DRVIFFRTEIDSLLWEILKTESSRANLSVGRLSGILIERFVNLNEMDVLCRCNLHR"
     gene            complement(852..1223)
                     /locus_tag="PK683_17825"
     CDS             complement(852..1223)
                     /locus_tag="PK683_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10364.1"
                     /translation="MFKKLGISLLILSFLSFGSLFAGDEEVDNTPEEEITDTKEKAAT
                     DANKKADPSAKKDEKKGKEKGKVKGKGKAKGKEKAKGKGKGKGKGKGKGKGKGKAKGK
                     GKAKGGTKKAAKEEAPAKKAE"
BASE COUNT          340 a          280 c          271 g          475 t
ORIGIN      
        1 ggccgtgaga gcaatgactc cttcacagtt tctggaaagc agatcatagt ctattctggg
       61 ttttctgtaa aatccttccg taaaagagcg gcttgagagc ttgattaaat tcttatagcc
      121 tacctcgttt tttgcgagca gtacaaggtg gtacgcattt ccgtccggaa tattttccag
      181 ttctgcttcc tcagacctgt ttgcagaaac ataaaattcc gaaccgatga tcggcttgat
      241 tccctgtttc agcgcctctt tatagaattc gattgcaccg aacatattgc cgtgatcaga
      301 aatccccact gaactcatcc cggatgcctt gacgtgtttc ataagatcag gtatacgtat
      361 tgccccgtcc aaagtggaat agattgtatg gagatgcagg tgggtaaaat ctgattcgat
      421 cacaagagac ataattgaaa atcgattctt ttcgtcaatt tcctttaaac atatcagggg
      481 tgcaattttc tgcaaaaaaa agagggaacg gacatttttc agacaggtca gaatatgccg
      541 gcaattttga cagaagtttc caagtcttat tttgacagat taattcagat gcagatgcac
      601 ccgaaggatg cagtccggta tggactgatt cttggcatat atgaatcaga atttgaagaa
      661 acggatgatc gagtcatttt ctttcgaacg gaaatagatt ctctgctatg ggaaatactg
      721 aaaaccgagt ccagtcgcgc aaatttaagt gtcggaaggt tgagcgggat tttgattgag
      781 aggtttgtaa atctgaatga aatggatgtt ctgtgcagat gcaatctgca cagataggca
      841 tagattatga attattctgc tttttttgca ggagcttctt ctttagctgc ttttttagtg
      901 ccgcctttag ctttgccctt tcctttagct ttgccttttc ctttgccctt gccttttcct
      961 ttgcccttgc cttttccttt gcctttagct ttttctttgc ctttagcttt tcctttacct
     1021 tttacttttc ctttctcttt gccttttttt tcgtcttttt tggcagaagg atcagccttt
     1081 ttgtttgcat cagtagctgc tttttctttc gtgtctgtaa tttcttcttc tggtgtgttg
     1141 tctacttctt catctccagc aaacaggctg ccaaagctca gaaaagataa aataagcagg
     1201 gaaattccta attttttgaa catactttaa tctccagtat tttgccggaa acgctccgac
     1261 ataaatttga ggacaggttc ttttgacccg cctaaaaaaa aaactatttt ttcctcgatt
     1321 tttacgggcg ttaaaaaatt agttctccaa gaatatcggc tttctg
//
LOCUS       DAOQGJ010001358         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76460_length_1366_cov_3.607933, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001358 DAOQGJ010000000
VERSION     DAOQGJ010001358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76460_length_1366_cov_3.607933"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..987
                     /locus_tag="PK683_17830"
     CDS             97..987
                     /locus_tag="PK683_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10365.1"
                     /translation="MKLSDFGFSSEYENICRLMFSGKRKSAFLEIEKSENREQYIPLL
                     CLDYAESIWFSALRRKKERNPARKLLQSLITPVQEDTLRHTALHFVWWSKFSLRLAEL
                     AKAASDFPLAPFWKEIVLLAFYKTGNGGGTCYNYGLLPISTVLNHIQEKNPYLADLSD
                     FFLQDIPDELALLNTRIINITDCPLTKMTFKKWRNLFVKQIIIRPNIPYRAMRVLRRT
                     FPEWDNAEILSQADYLLKFAEERKSVESQEAQKFGLYALNLLRLIPADYRKHTYYQKR
                     RQAYVSAGNERMAEFCSIHF"
BASE COUNT          393 a          273 c          297 g          403 t
ORIGIN      
        1 atgcggaaaa aagcataccg tctcgcagga cggggcaaga tggcggaata cagtgcagtc
       61 agatacggga tttaagagcg atgaaagctc cttattatga aactttctga ttttggtttt
      121 tcttctgaat atgaaaatat atgcagactt atgttctctg ggaaaagaaa atcagctttt
      181 ctggaaatag aaaaaagcga aaacagggaa cagtatatcc cccttctttg cctggattac
      241 gctgaatcca tctggttttc tgcattgcgc cgcaaaaagg aaagaaatcc ggctcggaaa
      301 cttcttcagt ctctgatcac tccggtacag gaagataccc tcagacatac tgcactccat
      361 tttgtctggt ggagcaaatt ttcccttcgg ctggcagaat tggccaaggc ggcttctgat
      421 tttccacttg caccgttctg gaaggaaatt gttctgctgg ctttttacaa gacaggaaac
      481 ggaggaggta catgttataa ttatggcctt cttccaattt ccactgtcct gaaccatatt
      541 caggagaaaa atccgtatct ggccgatctg agtgatttct ttcttcagga tattccggat
      601 gagctggctt tattgaatac cagaattatc aatattactg attgtcctct tacaaaaatg
      661 accttcaaga aatggagaaa tctctttgtg aagcagataa ttatccgtcc caatatcccg
      721 tatcgtgcca tgagggttct taggagaact tttccggaat gggataatgc agaaatcctg
      781 tcgcaggcag attatttgct gaaatttgca gaggaaagga aatctgtcga aagtcaagag
      841 gctcagaaat ttggactgta tgcactcaat cttctcagac tgatacctgc agattaccgt
      901 aagcatacat attaccaaaa gagaagacag gcttatgttt cagccggaaa tgaaagaatg
      961 gctgagtttt gttcaattca tttttaaggt ctttaattat gctgagctga ggcagactgt
     1021 ccaaggaatc agactgttgt actggaaata tccgacactg ctcagacagg tcagtctaga
     1081 aatgtaaata cttggattcc agaaatgaaa cagcacctta tcattttttt attgcttgta
     1141 tgctttggac tgccgagtat tgcagtagca tataaaaaga gagtttttaa gatgacatta
     1201 cctttaacgg ttgtattaag atcttccagg tcaaagcatt ttctatgtcc ggcactggga
     1261 gctgtatttt ttttactctc cgtatgcggc ccttccaggc agactgaacg gcttcagaaa
     1321 gatgcgatgg catcttttgg cataatccct tccccctctg cagaat
//
LOCUS       DAOQGJ010001359         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76489_length_1366_cov_2.596491, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001359 DAOQGJ010000000
VERSION     DAOQGJ010001359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76489_length_1366_cov_2.596491"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="PK683_17835"
     CDS             complement(<1..1265)
                     /locus_tag="PK683_17835"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH10366.1"
                     /translation="MSFFIIGCVYYSLNLHSAPSERNSDYSLILISADSLRKDRIGLK
                     RNGESITPNIDRFAKEAFQFADHHTTVPRTFASWADLLTGQYSMRHKVRDMFPSPSEA
                     ANIGSAEFPVIGQKLAERGFATAVFSSFAGDIFPRADFGFQTVHTADFNARILVVQKA
                     LEAQVFLLPIISGTVPGGEYFEEIRAFASRGDGAQYERPIKKFIQRNSGRPFFLTVFS
                     SVTHFPYSPPYPYYKKYADSKYYGKYKYFKFVDPTASEKPSQKEIQQIHDIFDSSVNA
                     FDAEFGRLIDMLKSQGIYEKSVIVLTADHGESLYEDVHGHGHGEHLRGESVTSIPLII
                     RFPEKFISDRIKTVCDEECRQFPDVSSSVDLYPTVMNLYGIEMTGSFPGKSLLTVLGK
                     KNWPEKREVYSETGVWFSDVGEHFFQKQR"
BASE COUNT          410 a          310 c          266 g          380 t
ORIGIN      
        1 attctctgct tctgaaaaaa atgttctcct acatccgaaa accatactcc tgtttcagaa
       61 tatacttctc ttttttcagg ccagttcttt tttccgagaa cagtcagaag ggatttcccc
      121 ggaaaactgc cggtcatttc aattccatac agattcatta cagtgggata cagatcgact
      181 gaactgctga catccggaaa ctgtctgcat tcttcatcac atacagtctt aattctgtct
      241 gaaataaatt tttcaggaaa tctgattata agcggtatgg aagtgacaga ttctcctctt
      301 aaatgttccc catgaccgtg tccatgcaca tcttcataca gggactcccc gtgatccgca
      361 gtcagaacaa ttactgactt ttcatagatt ccctgcgact tcagcatatc gatcagcctg
      421 ccgaattctg catcaaacgc atttacagag gaatcaaaaa tatcatgaat ctgctgtatt
      481 tccttttgac tgggcttttc cgatgctgtc ggatctacaa atttaaaata tttatatttt
      541 ccataatact tagaatcagc atacttttta taatacggat aaggaggcga gtaaggaaaa
      601 tgagtgacag aagaaaacac tgtcagaaag aaaggccgcc cgctgtttct ttgaatgaat
      661 ttttttatcg gtctttcata ctgtgcaccg tctcctctgg aggcaaatgc acggatttcc
      721 tcaaaatact ctcctccggg aaccgttccg gaaatgatcg gaagcagaaa aacctgtgcc
      781 tccagtgctt tctgaaccac taaaattctg gcattgaagt ctgcagtatg cactgtctga
      841 aatccaaaat ctgcccgagg aaatatatcg ccggcaaagg aggaaaagac tgcagtcgca
      901 aatcctcttt ctgcaagttt ctggcctatg acaggaaatt cagcacttcc aatatttgcg
      961 gcctcggaag gagaaggaaa catatctctg accttgtggc gcatggaata ctggcctgta
     1021 agaagatccg cccagctcgc aaatgttctg gggacagtag tatgatgatc ggcaaactga
     1081 aatgcttctt ttgcaaaccg gtctatattc ggagtaatgc tttcaccgtt tcttttcagc
     1141 cctatcctgt cctttcgaag gctgtccgca gaaatcagaa tcagactgta atcactgttt
     1201 ctctcagacg gggccgaatg cagattcaac gaataatata cacatccgat aataaaaaaa
     1261 ctcaataaaa ctgcagtgag cgcattcctg aatgaagtct gaaaggaaaa accctgcatg
     1321 agaatatatg cgaagaaaca ggaaaggata atgacagaat atccct
//
LOCUS       DAOQGJ010001360         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76596_length_1365_cov_2.645038, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001360 DAOQGJ010000000
VERSION     DAOQGJ010001360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76596_length_1365_cov_2.645038"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..271)
                     /locus_tag="PK683_17840"
     CDS             complement(53..271)
                     /locus_tag="PK683_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10367.1"
                     /translation="MYILSLVGMLLMYFLPVFFILGMFFPRYVLILSPSYRRTRLRVV
                     YLSLLMLALLITLNYVSQNKLPSFDLLK"
     gene            599..937
                     /locus_tag="PK683_17845"
     CDS             599..937
                     /locus_tag="PK683_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10368.1"
                     /translation="MNILWKHIFGILILSSVISAQDEPKFLELDFRECRSEKNRIYYA
                     FGSTAYEMKKGKEDTCIFRYGREVENPRWKGTLDTVCTVPSKTGRAKFKIENTYIDFS
                     EIKKYCSNVN"
     gene            complement(934..>1365)
                     /locus_tag="PK683_17850"
     CDS             complement(934..>1365)
                     /locus_tag="PK683_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10369.1"
                     /translation="QESNPTTDPQGTSSGFRYLPSMNLYQFCLYNTSYIYIRCSGNFS
                     FYIKKETFTYSASNTAERPAYPYLYGAFASLGVSRSVKPTRYYTRKSIESCRKKLWKE
                     VLAAYYYNLAQYKCSKSPKYINPASLASFHCDLQETAFGRF"
BASE COUNT          458 a          243 c          298 g          366 t
ORIGIN      
        1 gtatctgatt tttctaatta cgtatccctg aacacaggtg actgaaacga attcatttca
       61 gcagatcaaa ggaagggagt ttattctgtg aaacataatt cagtgtaatc agaagggcaa
      121 gcatcaggag agaaagataa acaactctca gtctggttct tctgtaagac ggagacagga
      181 taagcacata tctggggaaa aacatcccca gaataaaaaa cacaggaaga aagtacatca
      241 gaagcattcc gacaagcgag agtatataca taattacagt ctgccctgaa cttttctgaa
      301 gtaaagcaga acttgagact gtctcctgtt aaaaattttc ctcaagacag aattttcttt
      361 gtctaaaata aagataactg ctcagtaaaa aatacattgt aagagaattt atagagcaaa
      421 gtttaaaaag cggggactta gcggcttcat atgcttgcgg gggaagcaaa cccctcgcaa
      481 atttggaagc ggttaatctt cccgataggg acttgctttt taaaaagcaa tttcttgaaa
      541 aacggatatt tatgactgcg aagccgtcag gctgagcagt tacaataaag gagtcattat
      601 gaacattcta tggaaacaca tcttcggtat tcttatcctt tcatcggtta tttcagcaca
      661 ggatgaacca aaatttctgg aacttgattt cagggaatgt cggtcggaaa agaacagaat
      721 ctactatgca ttcggatcaa ctgcatatga aatgaaaaag ggaaaagaag acacctgcat
      781 attccggtat gggagggagg tggaaaaccc cagatggaaa gggactttgg atactgtctg
      841 cactgtccct tcgaagacag gaagagcaaa atttaaaata gagaacacat atattgattt
      901 ttcagaaatt aaaaaatact gcagtaacgt gaattagaat cttccaaaag cagtttcttg
      961 cagatcacag tggaatgaag ccagtgacgc tggattgata tacttgggag atttgctgca
     1021 cttatactgg gcaagattat aatagtaagc agccaaaacc tctttccaga gttttttcct
     1081 gcagctctca attgactttc tagtgtaata tctggtcggt ttgacagagc ggctcactcc
     1141 aagagaagca aaagcaccgt acagatacgg atatgcaggc cgctccgcag tattggaagc
     1201 tgaataggtg aaagtctcct tttttatgta gaaggaaaaa ttgcctgaac atcttatgta
     1261 tatatatgat gtattgtaca gacagaactg atagagattc atagacggaa gatatctgaa
     1321 tccggaactt gttccctgcg ggtctgtcgt tggattgctt tcctg
//
LOCUS       DAOQGJ010001361         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76774_length_1363_cov_2.625382, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001361 DAOQGJ010000000
VERSION     DAOQGJ010001361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76774_length_1363_cov_2.625382"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PK683_17855"
     CDS             complement(<1..1258)
                     /locus_tag="PK683_17855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH10370.1"
                     /translation="MILSLRKSNRYLKILNSEAESANRAKSEFLAVMSHEIRTPMNGV
                     IGMTGLLLDSGLNDVQRDYAETIRVCGDSLLMILNDILDFSKIESGKLELEKNPFSLL
                     ECIESGISLFEKQLREKCNKVSLTFHAECPVRIIGDQGRLRQIIVNLIGNAVKFTSDG
                     TINIEVSRGNRIESSENNIFYYQFRISDTGIGIPKDRMKRLFKPFSQADASSSRKFGG
                     TGLGLAISRKLALLMGGAMWAESAPGKGSDFYFTFQAEKQDLSENRDIFNDKKVLVHA
                     AESFRSEISEFLKLWNIQTLSTNKENFFALAESTDLIFLECTEFVSESEENRNRLKAL
                     VNSRKKPFVLISSDLRHCSAIAEDIYFLHTISPPLRQSRLYELVLGIFSGHFIISEQK
                     NFSVPENSGCLRILLAEDNSVNQKVAV"
BASE COUNT          336 a          309 c          289 g          429 t
ORIGIN      
        1 gtactgcaac cttctgattc actgaattgt cctctgcgag aagtattctc aggcatcctg
       61 aattttcagg gactgaaaag tttttctgtt cagaaattat gaagtgtccg ctgaatatcc
      121 cgagaacaag ttcgtagagc ctggactgac gaaggggagg ggatattgta tgcagaaaat
      181 aaatatcttc agcgatcgcg ctgcagtgtc tgaggtcgga agaaatcagt acgaaaggtt
      241 tttttcggga gttcaccagg gccttcagcc tgttccggtt ttcctccgat tctgaaacga
      301 attctgtgca ttcaagaaaa atcaagtcgg tgctttctgc cagggcaaag aagttttctt
      361 tattcgtgct gagtgtctgt atgttccaaa gtttcaggaa ttctgaaatt tctgaacgga
      421 acgactctgc agcatgaacc agtacttttt tatcattaaa tatatctctg ttttcactca
      481 gatcctgctt ctctgcctga aatgtaaaat agaaatctga gccttttcca ggagcacttt
      541 ctgcccacat tgcgcctccc atgagcagag cgagttttcg gctgatggca agtccaaggc
      601 cggtacctcc gaattttctg gaagaggagg catcggcctg actgaagggc ttgaacagtc
      661 tcttcatcct gtccttcggg attcctattc ctgtatctga tattctgaac tgataataaa
      721 aaatattatt ctcactgctc tcaatcctgt ttcctctgct gacttctatg tttattgttc
      781 cgtccgaagt gaattttaca gcgttcccga tcagattgac tatgatctgc cggagtcttc
      841 cctggtctcc gatgatcctg accgggcatt ctgcatgaaa agttaatgaa actttattgc
      901 atttttcccg gagctgcttc tcaaaaagac tgattcctga ctcgatgcat tccagcaggc
      961 tgaacggatt tttttccagt tcaagttttc ccgattctat tttagaaaaa tcgagaatat
     1021 cattgagtat cataagaagt gagtctccgc agactctgat tgtttctgca tagtccctct
     1081 gtacatcgtt caggccggaa tccagaagaa gacctgtcat cccgatcact ccgttcatag
     1141 gagttctgat ttcatggctc atcactgcga gaaattcgct ctttgccctg tttgccgatt
     1201 cagcctcgct gttcagtatt ttcaggtatc tgttgctttt tctgagagaa agaatcataa
     1261 atgtaagagt gcctaaaagt acggagatga gcattccgcc ggacattaca agcagctttc
     1321 ttcctgtctc ttccgatttc agaaattctc ttttcggaac cag
//
LOCUS       DAOQGJ010001362         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76864_length_1362_cov_3.167559, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001362 DAOQGJ010000000
VERSION     DAOQGJ010001362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76864_length_1362_cov_3.167559"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /gene="meaB"
                     /locus_tag="PK683_17860"
     CDS             complement(<1..652)
                     /gene="meaB"
                     /locus_tag="PK683_17860"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNH10371.1"
                     /translation="MNPEIKSLAERILSGERRALAKAITLLESTLSNDRRSAADLLHS
                     LPSENRNTIRIGISGVPGAGKSTFIESFGLFLISLGKKVAVLAIDPSSPISGGSILGD
                     KTRMPELTQNENAFIRPSPSKGSLGGIAARTRETILLCEAAGFDVILVETVGVGQSEV
                     AVSEITDFFLVLLIGGAGDELQGIKKGIMEMADLILINKADNTGKFSAENAKNEVNQ"
     gene            complement(649..>1362)
                     /locus_tag="PK683_17865"
     CDS             complement(649..>1362)
                     /locus_tag="PK683_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10372.1"
                     /translation="RHNDHIMKYLFFMIFMLQACAPAVYSKPDSSFYEVTSYSGKKKP
                     FPKDSIFLGFEEDFDFDPKQLEVFKNVQSIEIRNPSFESSAAGRLPSWQSLEYLNLSD
                     SGVTELSFTGKFPKLASIILNRTKVSEISSVSRNIPLTRLEAAESSLSNLSGIERFPF
                     LRRLDIRKTRVKDISMLSKNKKLYEFRAGGTEIQNITALYGMTELTYFEADNVSVTEE
                     SLKTLLSFNPYVKIVRRGR"
BASE COUNT          329 a          304 c          305 g          424 t
ORIGIN      
        1 cctggttgac ttcgtttttt gcattttcgg cagagaattt tcctgtattg tcagccttgt
       61 tgatcaggat caggtctgcc atttccataa tgcctttttt gattccttgg agttcgtccc
      121 ctgctcctcc gatcagaagc acaagaaaga agtcggtaat ttcagaaacg gccacttctg
      181 attgacctac gcctactgtt tccacaagaa tcacatcaaa tcctgcagcc tcacagagca
      241 gaatcgtctc ccttgttctt gccgctattc ctcccagact tccttttgag gggcttggac
      301 ggatgaatgc attttcattc tgagtgagtt cgggcatcct tgtcttgtct ccaagaatgc
      361 tgcctcctga gatgggtgag gacggatcaa ttgcgagcac tgccactttt ttcccgagag
      421 aaatcaggaa tagcccgaag ctttcaatga aagtggattt ccctgctccc ggaactccgc
      481 tgattccgat ccggatagta tttctgtttt ctgaagggag agaatgaaga agatctgctg
      541 cagatctcct gtcatttgag agagtacttt ccagcagggt aattgctttt gcaagagctc
      601 ttctttcgcc tgacaggatt ctttctgcaa gtgacttgat ctcaggattc atcttcctct
      661 cctgacaatc tttacatacg ggttgaagga aagaagagtc ttcagggatt cctcggttac
      721 ggaaacgttg tccgcttcaa aataagtcag ctcggtcatt ccgtaaagag cggtgatgtt
      781 ctgtatctct gttccgcctg ccctgaattc ataaagtttt ttatttttgg atagcataga
      841 gatgtctttc acgcgggttt tcctgatgtc gagtcttctc aggaaaggga atctttctat
      901 ccctgaaaga tttgataagg atgattctgc agcctccagt ctggtcagag gaatatttct
      961 gctgacagaa ctgatctctg atacttttgt cctgttcagt atgatgcttg ccagtttagg
     1021 aaattttccc gtgaatgaca gttcagtcac accgctgtcg gacaggttca gatattccag
     1081 ggactgccag gacggaagtc tgcctgccgc actgctttcg aaactgggat ttctgatttc
     1141 aatggactga acatttttaa aaacttcgag ctgctttgga tcaaaatcaa agtcttcttc
     1201 aaatccgaga aatatggaat ccttcgggaa aggtttcttt tttcctgaat atgaagtcac
     1261 ttcataaaag gaagagtccg gcttagagta tactgcagga gcgcaggcct gcagcatgaa
     1321 tatcatgaaa aatagatatt tcataatgtg atcattgtgc ct
//
LOCUS       DAOQGJ010001363         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76892_length_1362_cov_2.534047, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001363 DAOQGJ010000000
VERSION     DAOQGJ010001363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76892_length_1362_cov_2.534047"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..594
                     /locus_tag="PK683_17870"
     CDS             85..594
                     /locus_tag="PK683_17870"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004443082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNH10373.1"
                     /translation="MKKISVLFVCLGNICRSPAAEGAFLHLLEEKNLLGSFQVDSAGT
                     GDWHTGELPHEMTRKVAESRGIRLIHKARQFKAEDTKLFDYIIAMDDSNYRTVLRAAS
                     EEDKEKIMKFRRFDPTVNGEPDVPDPFYGGISGFEHVQDIVERCSENFLKWAVQKHPA
                     VLVPKENDK"
     gene            591..>1362
                     /locus_tag="PK683_17875"
     CDS             591..>1362
                     /locus_tag="PK683_17875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH10374.1"
                     /translation="MIFSLLEKNIFPDQIIRLGIRTLLAQRLQDEDRGSLEENQKHLM
                     NYIESLKKSPVAVNTLDANEQHYEVPTDFYRYVLGKRMKYSGGLWPEGTVSLDDSEER
                     MLALSCERAELKNGHTVLDLGCGWGSLSLYIAEKYPECRITSVSNSRTQKEYIDSVCR
                     ERGFKNVEVITKDMNEFSIDRKFDRLMSVEMLEHMKNYEKLFERFASFMKEDGLFFVH
                     IFTHSRFAYAFEAKDESDWMSKYFFTGGMMPSDSLFLYF"
BASE COUNT          422 a          277 c          329 g          334 t
ORIGIN      
        1 cccctcattt ccctggggaa tcatatacta tgcacttcag actatgcctg attttatatt
       61 caactacatc agcaggaaat acttatgaaa aaaatttcag ttctttttgt atgcctggga
      121 aatatctgca gatctcccgc agcggaaggc gcatttctgc atctgcttga agagaaaaat
      181 ctgcttggaa gtttccaggt ggattctgcc gggacaggag actggcatac cggcgaactg
      241 cctcatgaaa tgaccaggaa ggttgcagag tccagaggca ttcgtctcat tcacaaagcc
      301 agacagttca aggcggagga tactaagctt tttgattata taattgcaat ggatgattcc
      361 aattacagga cagtgcttag agcagcctcg gaagaagata aggaaaaaat aatgaaattc
      421 cgcagattcg atccgaccgt aaacggagaa cctgatgtgc ccgatccatt ttacggaggg
      481 atttcgggat ttgagcatgt acaggacatc gtggaaagat gttcagagaa ctttttaaaa
      541 tgggcagttc agaaacatcc ggcagttctt gtgccgaagg agaacgacaa atgatattca
      601 gccttcttga gaaaaacata tttccggatc agatcatccg gctgggaatc agaactctgc
      661 tcgcgcagag gcttcaggat gaagacagag gcagtctgga agaaaatcag aaacatctga
      721 tgaattacat cgagtcactg aagaaaagcc cggttgctgt aaatacactg gatgcaaatg
      781 agcagcacta tgaagtccct accgacttct acagatatgt tctgggaaaa agaatgaaat
      841 acagcggcgg actttggccg gaaggaaccg tcagcctgga cgattcagaa gaaagaatgc
      901 ttgcgctttc ctgcgaaaga gcagaactga aaaacggtca tacagtcctc gatctgggat
      961 gcggctgggg gtctctcagc ctgtacatcg ccgaaaaata tccggaatgc aggatcacct
     1021 ctgtttccaa ttccagaaca cagaaggaat acatagactc agtctgcaga gaacgtgggt
     1081 ttaaaaatgt ggaagtcatc acaaaggata tgaatgagtt cagcatagac agaaaattcg
     1141 acaggctgat gtccgtggaa atgctggagc atatgaaaaa ttatgaaaag ctgttcgaac
     1201 ggtttgcttc attcatgaaa gaggatggac tttttttcgt gcatattttc actcacagca
     1261 gatttgcata tgcattcgaa gcaaaggatg aatcagactg gatgtccaaa tattttttca
     1321 ccggcggaat gatgccctca gacagtctgt tcctgtattt cc
//
LOCUS       DAOQGJ010001364         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_76971_length_1361_cov_3.209801, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001364 DAOQGJ010000000
VERSION     DAOQGJ010001364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76971_length_1361_cov_3.209801"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PK683_17880"
     CDS             complement(<1..1052)
                     /locus_tag="PK683_17880"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNH10375.1"
                     /translation="MRNLDKLGSGRVLIYLTLILSLVFVDCGGKKKKGFLASTFWIAA
                     LTGTNPAKTTSNQNTTLDPNGVALPSQATSGASSQEVADHGPAVINGNLQVNNCTDAG
                     NNPIACSADSGLDLTQVTVSLIGADGTVVATTKPSADGNYSFNIPDLKNGDYRVLITQ
                     GNGVNAAYQDVNFSFNPVDSAQNTLTANLQTSRLYITSGPASITGMAVTPGFKDQTGT
                     TVVAAGPLPTGTTVQLLDNSGNVVATTTTNASGAYTFNIPNLSNGNYTVKALGSEQTS
                     NSQPFSDVSSGFQFTFLGNNPATTTNVNIPGLVSGWNPATSAVANLVNWSITNVAVSG
                     SDLSGFTVKLKDANGNV"
     gene            complement(1163..>1361)
                     /locus_tag="PK683_17885"
     CDS             complement(1163..>1361)
                     /locus_tag="PK683_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNH10376.1"
                     /translation="DIFINSFYSLQYSLCKYAIQGSDVYISPILENSSWYEFYRAKDF
                     IELGEKMGSEKISELKKLMKP"
BASE COUNT          383 a          287 c          284 g          407 t
ORIGIN      
        1 acatttccat ttgcatcttt cagttttaca gtaaaaccgg aaagatctga accggaaaca
       61 gcaacgtttg taatcgacca gttgaccaaa tttgcaacgg cacttgtcgc aggattccat
      121 ccggagacaa gtccaggaat attcacattg gtagtcgttg caggattatt tcccaaaaat
      181 gtaaattgga atccgctcga tacatcagag aaaggctgag aattggaagt ctgttcagat
      241 cccaatgcct ttacagtata atttccattt gaaagattgg gaatattgaa tgtatatgca
      301 cctgatgcat tggttgtcgt tgtggcaaca acattgccgg aattgtccag aagctgaaca
      361 gtggtacctg tcggcagggg ccctgcagca acaacggtag ttccagtctg atccttgaaa
      421 ccgggagtca ctgccattcc tgtaatggaa gctggacctg atgtaatata cagtctggat
      481 gtctgaagat ttgcagtgag ggtattctgt gcactgtcca cagggttaaa gctgaaattc
      541 acatcctgat aagctgcatt gaccccattg ccctgggtaa tcaaaaccct gtagtctcca
      601 ttcttgagat ctgggatatt aaaagaataa tttccgtctg cacttggctt tgttgtggca
      661 acaacagtcc catccgcacc aatcagtgaa actgttacct gagtcagatc aagtcctgaa
      721 tcagcagaac atgcaatcgg attgtttcct gcatctgtac agttgtttac ctgcagattc
      781 ccgttaatga cagccggacc gtgatctgca acttcctgag aagatgcacc tgaagtggct
      841 tgtgaaggaa gtgctactcc gttcggatcc aatgttgtat tctgattgga tgttgttttg
      901 gctggatttg tacctgtaag ggcagcaatc caaaacgtac tggccaaaaa gccttttttc
      961 ttctttccgc cgcagtcgac gaaaaccaga gataaaatca gtgttaggta aatcaatacc
     1021 cttcctgatc ctagtttatc taaatttctc atattgtact ccttttcaat taacttctat
     1081 taagtctgtt ttatgagacg tagccatatg atgaaagaaa tacattctgc gtcaattttt
     1141 tttcaaaaat tctgccattt aattaaggtt tcatcagttt tttcagttca gaaatctttt
     1201 cagaccccat tttctcgcca agttcaatga aatcctttgc cctgtaaaat tcataccagg
     1261 aagaattctc aagaatcggg ctgatgtaga catctgaacc ctggatggca tatttgcaga
     1321 gactgtactg gagagaataa aagctgttga tgaatatatc c
//
LOCUS       DAOQGJ010001365         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_77272_length_1358_cov_4.409056, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001365 DAOQGJ010000000
VERSION     DAOQGJ010001365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77272_length_1358_cov_4.409056"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK683_17890"
     CDS             complement(<1..182)
                     /locus_tag="PK683_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10377.1"
                     /translation="MKKFTVFIIMMYFLSGCRTESDVKRRQTTAAAAIALGASADDPG
                     EEYSAGNLTVFDDSSNA"
     gene            complement(210..>1358)
                     /locus_tag="PK683_17895"
     CDS             complement(210..>1358)
                     /locus_tag="PK683_17895"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNH10378.1"
                     /translation="VKAAEVPNFLSGYARYAFLAYSDSYSAAVDLQTKIKAFTGSPGE
                     STLKAAKAEWRKARISYLQTEILRFSSGPIDNKEILSVTKDGEKSELEPLINAWPLDE
                     TYLDNYISSSRTVSETELISANMNSAVVSSENDSAKNIAVGWHSIEYLLWGADASGNL
                     TAGTRPYTDYTSASNASRRVLYLNTVTDLLVKHIGLVKDSWDPDKSGNYAVSFKANPG
                     NTSLKNVMNGLVKFAKGEWGGERLKGLNTNSQEDEHSCFSDNSKNDAYYDAQGFINLY
                     TGNYKALKSVNETFSGLSTIAGSDSSIFTNAKTARDSYCLNENLEDSTQNQTCTSGSV
                     SSRYDSLLISTSNSERSILTGKVQTAIVSVGDSIAAIAKNYGIAIVSE"
BASE COUNT          343 a          314 c          270 g          431 t
ORIGIN      
        1 gcattggatg aatcatcaaa tacggtcaag tttcccgcag aatactcctc acccggatcg
       61 tctgcggaag cacccagagc aattgcggca gcagctgtgg tctgtcttct ttttacgtct
      121 gattctgttc tgcaccctga cagaaagtac atcataatta taaatactgt aaattttttc
      181 atagtcaatt ttcctgaaca tacaggctcc tattcgctta cgattgcaat cccgtaattc
      241 ttcgcaattg ccgcaataga gtctcctacg gatacaatgg ctgtctgtac ttttcctgtc
      301 aggatggatc tttctgaatt ggatgtgctg atgagaaggc tatcatacct ggaggatact
      361 gatccggagg tacaggtctg attctgagta ctgtcttcca gattttcatt cagacagtaa
      421 ctgtctctcg cagtctttgc atttgtaaag atggaagaat cactgcctgc gattgtactc
      481 agtcctgaaa atgtttcatt cacacttttc agtgccttat aatttccggt gtaaagattt
      541 atgaatccct gtgcatcata gtatgcgtcg ttcttggaat tgtccgaaaa acaggaatgt
      601 tcatcttcct gtgaatttgt attcagaccc ttcagtcttt ctcctcccca ttctcccttt
      661 gcaaatttca ctaaaccgtt cattacattc ttcagtgaag tattccctgg gttggccttg
      721 aaactgactg cataatttcc tgacttgtct ggatcccagc tgtcttttac aagaccgata
      781 tgcttcacaa gaagatcggt aacagtgttc agatataaaa ctcttctgga tgcatttgat
      841 gcagatgtat aatctgtata cggtcttgtc cctgcagtca aatttcctga agcatccgca
      901 ccccagagaa ggtattcaat cgaatgccat ccgactgcaa tattcttggc actgtcattt
      961 tccgaagaaa ccactgcaga gttcatattt gcacttatta attctgtttc tgaaactgtc
     1021 cttgaggaac tgatatagtt gtccagataa gtttcatcca ggggccaggc attgatcaga
     1081 ggctcaagct cagatttctc accgtccttt gtgacagaca gaatttcttt attgtctata
     1141 ggcccggaag aaaaacgcag gatctctgtc tgcagataag agattcttgc ctttctccat
     1201 tctgcttttg cggctttcag agtggattct cccggactgc ctgtaaatgc cttgattttg
     1261 gtctgaagat cgacagctgc agaatacgaa tcggaatatg ccagaaatgc atatcgtgca
     1321 tatccagaaa ggaaattcgg cacttccgcc gcctttac
//
LOCUS       DAOQGJ010001366         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_77325_length_1358_cov_2.712970, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001366 DAOQGJ010000000
VERSION     DAOQGJ010001366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77325_length_1358_cov_2.712970"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PK683_17900"
     CDS             complement(<1..751)
                     /locus_tag="PK683_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH10379.1"
                     /translation="MAQFIQIEESESVTVMKIKRPEALNALNETVLNEISETVSELEK
                     KKPGVVILTGEGKAFVAGADIAAMKTYSSAEAEKFAEKGQSVFNQIDQSANIYIAAVN
                     GFALGGGMELALACDIRIGSDKAKLGLPEVSLGLIPGFGGTQRMTRLIGSGRALEIIL
                     SADMISAEEGYRIGILNRLTTADELMNEAVKLAKTILSRGPVAIREAKRVSKRGLDLS
                     LEKGLELEKKAFGALFSGNEPKEGLGAFLEKR"
     gene            complement(922..>1358)
                     /locus_tag="PK683_17905"
     CDS             complement(922..>1358)
                     /locus_tag="PK683_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10380.1"
                     /translation="KRDNTGIRVMDRKALNQTQTLILKYSSNELLLFDHMDQKLYTLH
                     KFPKNKKVFLTREMTLRYVAEHFSYEDEKVFSSEAMSLILLKNKEFQSNDYTILNFEK
                     GNIKLQDFKPVYDLMDVFTLENELYIDVSYLKVYSDKKVSVE"
BASE COUNT          338 a          322 c          247 g          451 t
ORIGIN      
        1 ttcttttttc gaggaaagca ccgaggcctt ccttgggttc atttcctgag aaaagtgcgc
       61 cgaatgcctt cttctcaagt tccagccctt tttcgagaga aaggtccagt cctcttttgg
      121 aaactctttt ggcttcacgg atggcgaccg gtcctctgga cagaattgtc ttcgcaagct
      181 tgaccgcttc attcatcagt tcatctgctg tcgtcagtct gttcaggatt ccaattctgt
      241 atccttcttc cgcactgatc atatctgcac ttaatataat ttccaatgcc cttccggaac
      301 cgatcagtct ggtcattctt tgagttccgc caaacccggg aatcagtcca agagacacct
      361 caggcagtcc aagttttgcc ttgtctgatc cgattcgaat gtcacaggcg agtgcgagtt
      421 ccattcctcc gcccagagca aaaccgttga ccgcagcaat ataaatattg gcagactggt
      481 ctatctgatt gaatacagac tgtccttttt cagcaaattt ctcagcctcg gcactggaat
      541 atgttttcat ggccgcaatg tcagcacctg ccacaaatgc cttcccttca ccggtcagaa
      601 tcaccactcc tggttttttc ttttccaatt ccgaaactgt ctctgaaatc tcattcagaa
      661 ctgtttcgtt caaagcattc aatgcttccg gtcttttgat tttcatcact gtaactgatt
      721 ctgattcttc gatctgaata aactgtgcca ttctttcctc tttgaaggtt gctgtttaca
      781 aaatgaaaat aaggaacagt gtgtaaagtg atttttagga agaggtgacc ttgcaggcga
      841 attatcatcc cctctctttc gactgggtct tctgctgttt ctgcacagcc tccgcttttc
      901 attgtatttt caagagccca cctattcaac ggaaactttc ttatcagaat atactttcag
      961 ataagataca tcaatgtata attcattctc cagagtaaag acgtccatca gatcgtacac
     1021 tggtttaaaa tcctgcagtt ttatatttcc tttttcaaaa ttcagtattg tatagtcatt
     1081 tgactgaaac tctttgttct tcagaagaat aagactcatt gcctcagaag agaaaacttt
     1141 ctcatcctcg taagaaaaat gctcggcgac atacctcagc gtcatctccc gtgtaagaaa
     1201 tactttttta tttttgggaa atttatggag agtatataat ttttgatcca tatgatcaaa
     1261 gagcagaagc tcatttgaac tgtattttaa aatcagagtc tgggtctgat tcagggcctt
     1321 tctgtccatg actctgattc ctgtattgtc tcgtttat
//
LOCUS       DAOQGJ010001367         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_77387_length_1357_cov_4.713518, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001367 DAOQGJ010000000
VERSION     DAOQGJ010001367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77387_length_1357_cov_4.713518"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1290)
                     /locus_tag="PK683_17910"
     CDS             complement(268..1290)
                     /locus_tag="PK683_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNH10381.1"
                     /translation="MSTESLFEEQKRREKLEKLGDPLKKLSELIDFEIYRKDLEKMYH
                     AGKEKGGRPPYDPVKMFRILIIQRLYGLSDAQTEFQINDRRSFQKFIGVSEYESLPDE
                     KTIWVFRERIKESGLDKVLFSKLLYWIRKLGFSLNEGMIVDATITAVPIQRNTKEENE
                     TIKKDMIPEGWDEEENMRKTAQKDMDARWTKKHGKSYYGYKNHIAADRKTKIIRDFEV
                     TDASVHDSQMYEELLQAAEKDETVFGDSAYSAEDLKKKTEKHSSFPLMCRKGCRNRTL
                     TEIEKQWNRILSKVRSRVEHIFADIKSFGAGRIRTVGMERARVQIALANIVYNMRRVS
                     FLLRSK"
BASE COUNT          331 a          318 c          222 g          486 t
ORIGIN      
        1 aagtaaatta atattatttt tttatatgta taaaaaattt ttatgcattt ttttaaaaca
       61 tataattttt ccatacctgc tcctgcttga acattgagca aagataatga ttatcaatct
      121 caatataata agctgatggg cacctctaaa aacagcttcc ttggatacga tgaaaaagat
      181 acggccgatc atttctgctt cgataacgaa atttcattaa gaaaaatgcg ggaaaacgta
      241 gattgtactg atttcagacg gtcatcttca ttttgaccga aggagaaatg atactctgcg
      301 catattgtag actatgttcg ccaatgcaat ttgaactctg gctcgctcca ttcctaccgt
      361 tctgattctg cctgctccaa atgatttgat gtctgcaaag atatgttcga ccctgcttct
      421 gactttggaa agtattctgt tccactgttt ttcaatttcc gtgagagttc tgtttctgca
      481 tccctttctg cacatcagag gaaacgaact gtgcttctcc gttttcttct tcagatcttc
      541 tgccgaatac gctgagtctc cgaatacagt ctcatctttt tcagcagcct gcagaagctc
      601 ttcatacatc tgagaatcat ggacggaagc atccgtcact tcaaagtccc tgatgatttt
      661 tgtcttccgg tctgccgcaa tatgattctt gtatccgtaa tacgatttcc cgtgtttctt
      721 tgtccagcgt gcatccatat ctttctgtgc agtttttctc atattctctt cctcgtccca
      781 tccttcagga atcatatctt tctttattgt ttcattctct tctttcgtgt ttctctgaat
      841 cggaaccgct gtgatcgtcg catccacaat cattccctca ttcaggctga aaccaagttt
      901 cctgatccag tagagcagct ttgaaaacag aactttgtcc agtccggact cctttattct
      961 ttctctgaaa acccatattg tcttttcatc cggcagagat tcatactcac tgactccgat
     1021 aaatttctga aatgaacgcc tgtcatttat ctgaaattcc gtctgtgcat cagacagacc
     1081 ataaagtctc tggattatca gaatcctgaa catcttcaca ggatcatacg gaggacggcc
     1141 tcctttttct ttgccggcat gatacatttt ctccaagtct ttacggtata tttcaaaatc
     1201 aatcagttca gacagcttct tcagcggatc tcccagcttt tccagtttct ctctgcgttt
     1261 ctgctcttca aacaggcttt ctgtgctcat atattcagtt tattttttca tctcttttgt
     1321 tgactctttt tttatcaggg tttttagagg tgcccta
//
LOCUS       DAOQGJ010001368         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_77400_length_1357_cov_3.316436, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001368 DAOQGJ010000000
VERSION     DAOQGJ010001368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77400_length_1357_cov_3.316436"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1279
                     /locus_tag="PK683_17915"
     CDS             119..1279
                     /locus_tag="PK683_17915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HNH10382.1"
                     /translation="MFTLKSVAQIQEEYRKSLIEEYSKTKEKSAQFEKYRDAGSLAGM
                     ESYLNSKAELTYFQDMQKELEKTPEKKIEEFQKMTSVSLQNDFPEEDFRFIEWERTAA
                     DKRKLDSVLKDIIRSIQENQKKLNENYLRSESDLKHYMHSVKSPKVTGVGNELVKKKT
                     PESVIKKFRQAEEDLETVWKEGFELYRLLTYKSVIMRSDGLVSYTHFHEPGISYFNLI
                     DRNYFEVIDDLIHENAHHHLNLLFKKYKILRNEKTEHMFYSPWRDSLRPLYAILHACF
                     TFTWGSMLFFHICTSNSLKYTDLKEKDVSYSAYRFLAESLMLDYSLNDLESEKKLFTP
                     EGKEILDFLIGWNRTSFDFRKVLEKRTDGSLLAKAETLRKHLNKMKKQYVRK"
BASE COUNT          466 a          252 c          272 g          367 t
ORIGIN      
        1 ccacaaaaaa ttttcggtct gcaccggatg ctgatcagga aaggatttct cttaatttct
       61 tttcagctga atttttacag acttgaccgt cgatgccgat tcaattttct atcttactat
      121 gtttacatta aaatccgtcg cacagattca ggaagaatac agaaaaagcc ttattgaaga
      181 gtacagtaag acaaaggaaa aatcggcaca gtttgaaaaa tacagagatg caggctcact
      241 tgcaggaatg gaaagttatc tgaattcaaa ggcagaactg acttattttc aggatatgca
      301 gaaagaactc gaaaaaacac cggaaaagaa aattgaagag tttcagaaaa tgacttcagt
      361 gagtcttcag aatgatttcc cggaagaaga tttccgtttc attgaatggg aaagaacagc
      421 agcggataaa agaaagctgg attcagttct caaagacatc attcgttcaa ttcaggaaaa
      481 tcagaaaaaa ctgaatgaaa actatctcag atccgaatct gatctgaagc attatatgca
      541 ttcagttaaa agtcctaaag tgaccggagt gggaaatgaa ctcgtcaaaa agaaaacgcc
      601 cgaatcagta atcaaaaaat tcagacaggc ggaagaagat ctggaaactg tatggaagga
      661 aggatttgag ctttacagac tgcttactta caagtcggtc ataatgagat cggacggact
      721 ggtcagctac actcattttc atgaaccggg aatttcttat tttaatctga ttgacagaaa
      781 ttattttgaa gtcatcgatg atctgattca cgaaaatgct catcatcatc tgaatctgct
      841 cttcaaaaaa tacaaaatat tgcggaacga aaaaacagag catatgtttt attctccctg
      901 gagagattct ctgcgtcctc tttatgccat actccatgca tgttttactt tcacatgggg
      961 ttcaatgctt ttctttcata tctgtacttc aaactctctg aagtatacag atctcaagga
     1021 aaaggatgtc tcttattcgg catacaggtt tcttgcagaa tcactgatgc tcgactattc
     1081 tctgaatgat ctcgaatcag aaaaaaaact tttcactcct gaaggaaagg agattctgga
     1141 tttccttatc ggatggaaca ggacttcatt tgatttcaga aaggttcttg aaaaaagaac
     1201 cgacggttct ctgcttgcta aagcggaaac actccgaaaa catctgaata aaatgaaaaa
     1261 gcagtatgtc agaaagtaaa attctttcac aggcccgcga attcggattt gacctggcgg
     1321 gcgtctgcag agccgagatt ccggaacagg acagaaa
//
LOCUS       DAOQGJ010001369         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_77489_length_1356_cov_3.915450, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001369 DAOQGJ010000000
VERSION     DAOQGJ010001369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77489_length_1356_cov_3.915450"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..>1356)
                     /locus_tag="PK683_17920"
     CDS             complement(186..>1356)
                     /locus_tag="PK683_17920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNH10383.1"
                     /translation="LLNLANLFLFKYFYFFCDMAYLLTGKNEAVREFSTFVHIALPLA
                     ISFYTFQIIAYQADIHRGKILNSISFTDYSVFILFFPQLIAGPIMRSEEFLPLLEKPV
                     YSDDYVKNGILLILTGLLKKVVIADTVSGIINPVFSAHNEYSGPALFLATLGFAIQVY
                     SDFSGYSDMARGLAFLLGFDIPVNFRGPFLSQSFTELWSRWHITLSTWLRDYLYIPLG
                     GNRDGHSGRNMMITMSLGGLWHGANISFFLWGFYLGLLLYIERVFVQFRKKEGDPAVK
                     KFSIFRAVPVFIIFSLSGVFFRTGILGINSILVCYEFYLRMFSFSAGKMVYRYEELIF
                     YILFGYLLNALEYSETALSGLRKFREILIPVYSVCILLLLGLFGDGGGDFIYFQF"
BASE COUNT          457 a          288 c          263 g          348 t
ORIGIN      
        1 ttcgatttca tcggtgatcg gggatgcgtc ttctttttcc aaagtcctgc cattttcaga
       61 tttcttactt cttttttttc accgggaagt ctcctgtacc attctccgcc ccctgcagaa
      121 caggattcca gaaaaaaaac aagaatcagg aaaggactgt ttcttttctg aaaaatcatt
      181 gcactctaaa actggaaata aataaaatca ccgcccccgt ctccaaagag tcctagaagc
      241 agaagaatgc agacggaata gactggaatc agaatctctc tgaattttct gagaccggaa
      301 agtgcagttt ctgaatattc aagtgcattc agcaaatacc cgaaaagaat ataaaaaatc
      361 agttcctcat atctgtacac catttttccg gctgaaaaac tgaacatcct cagataaaac
      421 tcatagcaga ccaggattga attgattcct aaaattccgg ttctgaaaaa aactcccgac
      481 agactgaaaa tgatgaacac aggcactgca cgaaagatgg aaaacttttt aaccgcagga
      541 tctccttctt ttttccggaa ctgtacaaag actctttcga tataaagaag aagtccaaga
      601 taaaatcccc ataggaaaaa actgatattt gcaccatgcc agagtcctcc gagactcata
      661 gtgatcatca tatttctacc gctgtgaccg tccctgttgc cccccagagg gatgtacagg
      721 taatcccgga gccaggtgga aagagtgata tgccatctcg accagagttc agtaaatgac
      781 tgagaaagaa aaggtcctct gaaatttaca ggaatgtcaa atcccaaaag aaaagccagt
      841 cctcttgcca tatctgaata accgctgaaa tcagaataca cctgaatggc aaaaccgagt
      901 gtcgccagga aaagcgcagg ccccgaatat tcattgtgag ctgaaaacac aggattaatg
      961 attcctgaaa cagtgtctgc gatgacgact tttttcagaa gtcccgtcag aatcagaagt
     1021 atgccgtttt ttacatagtc atctgaatat acaggttttt ccagaagagg aagaaattcc
     1081 tccgatctca taatcggacc ggcaatcagc tgaggaaaga aaagtatgaa tacagaatag
     1141 tctgtaaacg aaatggaatt cagtattttt cctctgtgaa tgtccgcctg atatgcaatg
     1201 atctgaaatg tatagaagct tatcgcaaga ggaagtgcga tatgaacgaa tgtggaaaat
     1261 tctctgactg cctcattttt tcctgtcagt aaatatgcca tatcacagaa aaagtaaaaa
     1321 tatttaaata agaaaagatt tgcaaggttc agcagg
//
LOCUS       DAOQGJ010001370         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_77628_length_1355_cov_2.965385, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001370 DAOQGJ010000000
VERSION     DAOQGJ010001370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77628_length_1355_cov_2.965385"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..914)
                     /locus_tag="PK683_17925"
     CDS             complement(24..914)
                     /locus_tag="PK683_17925"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNH10384.1"
                     /translation="MIFYNNTTYILRHIFAALGIFILTGCTGWPLLNHALQLQKTEKK
                     DMNPLLLLLLQNRGSSSSGTSTSSSLTAPTLLTASGSSGKVSLTWSSVTGASSYTVYY
                     STSSSVTVSSASVTAGNVTAYTVQSLTNGTLYYFAVKAASASASSALSNILSATPSSG
                     ASVQCLSQGYCNTFVASAGYNGNLGGLSGADAICSGAKPGGLPGTGTDYKAMLVVVGS
                     RDYPSTNWVLYANMQYRQADAVTVIGTTDANANFTTALTNPINPGGAAAAVWSGWDKT
                     NTCTGWTSTAGNGDQGPAAG"
     gene            complement(1128..>1355)
                     /locus_tag="PK683_17930"
     CDS             complement(1128..>1355)
                     /locus_tag="PK683_17930"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH10385.1"
                     /translation="AASDFSKIKHPVLILHGKNDSWIPPEESEKLSQEIQGSSLTVIE
                     HSGHCPMETHPDTVNKLILSFINNSSLPKIN"
BASE COUNT          349 a          309 c          318 g          379 t
ORIGIN      
        1 atccactgct gcaggaattg cagtcagccc gcagcaggcc cctgatcacc gttgcctgca
       61 gtcgaagtcc atcctgtgca tgtattcgtc ttgtcccagc ctgaccatac cgccgctgca
      121 gcacccccag gattgattgg atttgtaagt gcagttgtga aattggcgtt cgcatccgtg
      181 gttcctatga ctgtcactgc atctgcctgt ctgtactgca tatttgcata aagcacccag
      241 tttgtggaag ggtagtctct cgatcctacg acgacaagca ttgccttata gtctgtcccc
      301 gtgccgggaa gtcctccggg ctttgctcct gaacagatgg catctgcacc ggacagacct
      361 cccaggtttc cgttgtatcc tgcactggcc acaaatgtat tgcagtaccc ctgactcaga
      421 cactgcacgg aagcacccga cgagggagtt gcgctcagga tattggaaag cgcagaggag
      481 gcggaggcac tggctgcctt tactgcaaaa taataaagag ttccattagt aagactctgt
      541 actgtatatg cagttacgtt tccggcggtt actgaagcgg aagagacagt gacacttgaa
      601 cttgtgctgt agtagactgt gtaggaagag gctcctgtga ctgaactcca tgtaagactg
      661 acttttccag atgaaccgga agccgtcagg agagtcggtg cagtcagtga ggacgatgtc
      721 gatgtgcctg atgaactgct tcctctgttc tgaagcagca gaagaaggag gggattcata
      781 tctttctttt cggtcttctg cagctgaagg gcatgattca gaagaggcca tcctgtacag
      841 cctgtgagaa tgaatattcc caatgctgcg aaaatgtgtc ttaaaatata agtggtatta
      901 ttataaaata tcaagatatt tttcctgaga attttctttg gaaatcagtc atctgccgaa
      961 acactcctgt tcagactgca gctgcagaat tgttctgttt tcaaattaca taacgtttct
     1021 cattccgttc tttttcagat ttttgaaaaa tcctgaatca taagcataaa aaaataaggc
     1081 tctgctgatt tcagaaattc agaatcattc cagagtccgt gcaaaagtca gtttatctta
     1141 ggcaaagaag agttattaat aaaactcagt ataagtttat tcactgtatc aggatgagtc
     1201 tccatcggac agtgacctga gtgttcaatc actgtaagag agcttccctg aatttcctga
     1261 ctcagttttt cagattcttc cggcgggatc caggaatcat ttttcccgtg aaggatcaga
     1321 accggatgct taatctttga aaagtccgat gctgc
//
LOCUS       DAOQGJ010001371         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_77755_length_1354_cov_2.645881, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001371 DAOQGJ010000000
VERSION     DAOQGJ010001371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77755_length_1354_cov_2.645881"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1354)
                     /locus_tag="PK683_17935"
     CDS             complement(<1..>1354)
                     /locus_tag="PK683_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH10386.1"
                     /translation="LDDILPVGGADTTVTVTVDMEALKRDLDNSASKILNFAKIPQEQ
                     VKEFTALLAKLKTLKNPFDADNETRKIRKNLIKTYWDVYSSCIQKYITGSHNVPKPVL
                     MMLKYGYFDETLLEPNQVMYLYTAEDTEKGAPNIPIHMGTEWLEQIYNKNFVTSLDEL
                     GQTYFDKIKNDYKDMPFKKESDVPANIDTSENRLKYEMNAMYQPNVRLTTGNPGNFLP
                     ILTKFHITLPLDKTIVTRKALSDTLNEILQIDYSAFNREVMINNEQIRKEFVQRSIIP
                     DFILVPSIGSKIMMWQDLSVLRGAGSKESRGRIIFPIFILGDLKTMMLEAIAAFRWEL
                     CKNILGPEWNNVGVPSITSEYMDYIQFFKKNKDLSIEIKEKIAAEFKRFRTDRDKFVN
                     DYMLWIKYESEGIQRLNKVVRGIFYKHIPFQKDIRDKVSKLPAYADMHNRFTNIRNRQ
                     F"
BASE COUNT          338 a          283 c          286 g          447 t
ORIGIN      
        1 gaactgtctg ttcctgatgt tcgtaaaccg gttgtgcata tctgcatatg caggaagctt
       61 ggatactttg tcccggatgt ctttctgaaa agggatatgc ttgtagaaga ttccgcgtac
      121 aaccttattc agtctctgga ttccttccga ttcatatttg atccagagca tataatcatt
      181 tacgaatttg tccctgtctg ttctgaaacg cttgaattcg gctgcaatct tctccttgat
      241 ttcaatcgaa agatccttgt tcttcttgaa gaattgtata taatccatat attctgaggt
      301 aatagaagga acacctacgt tgttccactc agggccgagg atattcttac agagttccca
      361 gcggaatgct gcaatcgctt ccagcatcat tgtcttcaga tcgcccagta tgaagatagg
      421 gaatatgatc cttccacggc tttccttgga accagcacct ctcagaacag aaagatcctg
      481 ccacatcata atcttgcttc ctatggaagg aacaagtatg aagtcaggaa tgatgcttct
      541 ctgaacgaac tcttttctga tctgttcatt attgatcatc acttccctgt tgaaagcact
      601 gtagtcaatc tgcagaatct cattcagtgt gtctgaaaga gcctttcttg tgacgattgt
      661 cttgtcgagc ggaagtgtga tatggaattt tgtaaggata ggaagaaagt ttccaggatt
      721 tcctgtagtc agtcttacgt taggctgata catagcattc atttcatact tcagacggtt
      781 ctcgctggta tcaatgtttg cagggacatc cgattctttt ttgaagggca tatccttgta
      841 gtcgtttttg atcttgtcaa aataggtctg tccgagttca tcgagggatg tgacaaagtt
      901 cttgttgtag atctgctcaa gccattcagt tcccatatga ataggaatgt tcggtgctcc
      961 tttttctgtg tcctctgcag tgtacagata cattacctga ttaggttcaa gaagagtctc
     1021 atcaaagtat ccgtacttga gcatcatcag taccggcttt ggaacattgt gacttcctgt
     1081 aatgtatttc tgaatgcagc tggaatagac atcccagtaa gtcttgatca gattctttct
     1141 gatctttctt gtttcattgt cagcatcaaa aggatttttt aaagtcttaa gtttggccag
     1201 aagagctgta aactccttga cttgttcctg aggaattttg gcaaagttca gaatcttgga
     1261 tgcggagttg tccaggtctc ttttcagagc ttccatatcc acagtcacag tcacagttgt
     1321 atctgcaccg cctacaggca ggatatcgtc gagc
//
LOCUS       DAOQGJ010001372         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_77955_length_1352_cov_3.178874, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001372 DAOQGJ010000000
VERSION     DAOQGJ010001372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77955_length_1352_cov_3.178874"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..727)
                     /locus_tag="PK683_17940"
     CDS             complement(95..727)
                     /locus_tag="PK683_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10387.1"
                     /translation="MAYSTLSLLFLFSMNLSGNSLKFPKESDPDIKKFVPAGYVLTKE
                     ATGDLNQDGLEDKVLVLEKSPNESRVLVILFKDITGKFSYFTHSEKLILPMSEGDAFG
                     DPCSSVTVEKGLLVINHNAGKLQRWGMRYSFQFQSGHFYLTSRMTVSLSLNSSREARK
                     NEYFPQSGVNIETMTDASGKEKKIRLKMKKRPHLLLSEFKGYSIRVDDEF"
     gene            complement(933..>1352)
                     /locus_tag="PK683_17945"
     CDS             complement(933..>1352)
                     /locus_tag="PK683_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004508690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNH10388.1"
                     /translation="LDVPTTAHILGGAIIGETENQGVVDLQNRVFGYKNLMVCDGSMV
                     PANLGVNPSLTITALSERAMSYVPPKEGKEQKFFQYEKSWSITDVLKRTKSSVQIPLK
                     SETVSITDNKKTKEKKKAVSGKTAKKKTASKSKTKKK"
BASE COUNT          383 a          296 c          244 g          429 t
ORIGIN      
        1 gtttccctgt ataaactcta atctaaccgt tctaaaatac aaagcaataa tcaggccaaa
       61 aatgtaaatt ttttttcttt aaactgaaaa tttcttagaa ctcgtcatct acacggattg
      121 aataaccttt gaactcactc agaagaagat gcggtctttt tttcattttg agtctgattt
      181 ttttttcctt tccggaagcg tcagtcattg tttctatatt gactccgctt tgggggaaat
      241 attcattttt cctcgcttcc ctggaagaat tcagactcaa ggaaactgtc atcctactgg
      301 taagataaaa atgacccgac tgaaactgaa aactgtatct catcccccac ctctgaagtt
      361 ttccagcatt atgattgatc acaagaaggc ccttctccac tgttacgctc gaacatggat
      421 caccgaatgc atctccctcc gacatcggaa ggatcagttt ttctgaatga gtaaagtaag
      481 aaaattttcc tgtgatatcc ttaaaaagaa tgacgagaac cctgctttca ttcggtgatt
      541 tttcgagaac gagaactttg tcctccagtc catcctgatt caaatcgcct gtcgcctcct
      601 ttgtgagaac gtaaccggca ggcacaaatt tctttatgtc cggatcagat tctttcggaa
      661 acttaagact gtttccggaa agattcattg aaaaaagaaa caggagtgat aaagtggaat
      721 atgccatatc cctatttaaa tatgattcag tatccggtaa acaaagattt tttaactgcg
      781 ggaatatgct cacgtaaatt cgcaatctca gtaaaacacc gaaaatacac cgtttgtttc
      841 tgcaggagta aataatcagt tcaacataga caggagaact cagacagacg cggcatccct
      901 ttaaaaaccc aaggaatgcc gcattcagaa gattatttct ttttcgtttt tgattttgaa
      961 gcagtctttt tctttgcagt ttttccggaa acagccttct tcttttcttt tgttttctta
     1021 ttatcagtaa tactgacagt ctctgatttt aatggtatct gaacagatga tttcgtcctc
     1081 ttcagaacat cagtaatgct ccaggatttt tcatactgaa agaatttctg ctcttttcct
     1141 tccttgggag gaacatagga cattgctctt tctgaaagtg ccgtgatggt aaggctgggg
     1201 ttgacgccca gatttgcagg aaccattgac ccgtcgcata ccatcagatt cttataaccg
     1261 aaaaccctgt tctgcagatc cacaactccc tggttttccg tttctccgat gatggcacca
     1321 cccagaatat gtgctgtagt ggggacatcc ag
//
LOCUS       DAOQGJ010001373         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78048_length_1351_cov_4.352623, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001373 DAOQGJ010000000
VERSION     DAOQGJ010001373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78048_length_1351_cov_4.352623"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1022
                     /locus_tag="PK683_17950"
     CDS             99..1022
                     /locus_tag="PK683_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10389.1"
                     /translation="MNNTFRNFIIVFLISSSLLFSQEIGLPFPDEPEPKWKEKDSVPL
                     PNGSNNSSPSENKKESESKNPDSAESPLPNENSVKQNNPSLKNNEKGKPKSLSEKPPL
                     GTYPRGTYHLNRQDLNRAVPEFTEGTSKEGATSVYSKFEMIRLLSRDRKKDQAKTMAD
                     TLEEGWKLKAYFEIASGLESTAYTKSEKEESIPFYLQVITEISKDEKDSVMIQLAAKS
                     HWALANLLFKLGDHIPALDHLSRIILDFPGSNYLDGAYYLSGKIYEEGLPGKVRDLER
                     AKKYYQLFLSKKDQYKNSIYTKEVEKRLKSL"
     gene            complement(1027..>1351)
                     /locus_tag="PK683_17955"
     CDS             complement(1027..>1351)
                     /locus_tag="PK683_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNH10390.1"
                     /translation="IGVVGSYKGEIVYSMNLDAAYKISKLLVPSLDNESVKDEYKDII
                     GEIGNMTTGNALRIFASNGQFVDITTPNVMEVSTANIQYKKTTTLSLNLYSRIGQIEI
                     NIAIL"
BASE COUNT          453 a          264 c          266 g          368 t
ORIGIN      
        1 ggatacagga cttttgaaaa atgaaactcc tgaaaaaact gaaaaaaccg ggcggttgat
       61 taaatcgggc aaaagaaaag gcgcacagat tctccaaaat gaataacaca tttagaaatt
      121 tcatcattgt ttttctgatc agcagttccc ttcttttctc acaggaaatc ggtctgccct
      181 ttccggatga gccggaaccg aagtggaaag aaaaggattc tgttcctctt ccgaatggtt
      241 caaataacag cagtccttca gaaaataaaa aggaatcgga atcaaagaat ccggattcag
      301 cagaatctcc tctcccaaat gaaaattccg taaaacagaa caatccatca ttaaaaaata
      361 atgaaaaagg aaagcccaaa tccctttctg aaaaaccgcc cctcggaacg tatcctagag
      421 gaacctatca tttgaacaga caggatctga acagagccgt acctgagttt acagaaggaa
      481 cttccaaaga aggtgcgacc tccgtttatt caaaatttga gatgatccgt cttctttcga
      541 gggacaggaa aaaggatcag gctaaaacga tggctgatac tctcgaagag ggctggaagc
      601 tgaaggctta ttttgaaatt gcttctggac tcgagagcac tgcgtataca aaatctgaaa
      661 aagaagaaag tattccattt tatcttcagg tcattacaga gatttccaaa gatgaaaaag
      721 attcagtaat gattcagctt gctgcaaaat ctcattgggc acttgcaaat cttctcttta
      781 agctggggga tcatattccc gcattggatc atttgtccag aatcattctt gactttcccg
      841 gatccaatta tttggacggt gcgtattatt taagcgggaa gatatacgaa gaaggacttc
      901 caggtaaagt gcgtgatctg gaaagagcaa aaaaatacta ccagctgttt ttgagcaaaa
      961 aagatcagta taaaaacagc atatacacaa aagaagtcga aaaaaggctt aaatcacttt
     1021 aaagaattag agaatcgcta tattgatctc tatctgacct attcttgagt agagatttaa
     1081 tgagagtgtt gtagttttct tgtactgaat atttgctgta ctgacctcca ttacatttgg
     1141 ggttgtgata tccacaaact gaccgtttga agcaaatatt ctgagggcat taccagtcgt
     1201 catattccca atttcaccta tgatatcctt gtattcgtct tttactgatt cgttgtccaa
     1261 ggaaggcaca agaagcttgg agattttata tgcagcatcc agattcatac tgtaaacaat
     1321 ctccccttta taggaaccga ctactccgat g
//
LOCUS       DAOQGJ010001374         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78066_length_1351_cov_3.175154, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001374 DAOQGJ010000000
VERSION     DAOQGJ010001374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78066_length_1351_cov_3.175154"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PK683_17960"
     CDS             complement(<1..685)
                     /locus_tag="PK683_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10391.1"
                     /translation="MFVLRLLLFVMLFQAVRCRGYWKCESGDCSSGTAKLSTRYCDQY
                     EGGVENGEPKGYGKFTYSGNKCPEPGFYRLEPEGLVYEGNWGIWKHGYNRLIGEGKII
                     YPNGAAYGGTFDGDDAPKGKGWVVLPGQKKVEGIWKRRRLCTSGDCMNGPGVHYYVGS
                     VSYRSETEFKNGVSSGKCKMDYFRLIEGNPLKTLRYSSVGECTDLLFSGKVVDCDKNE
                     PELCRKIMKK"
     gene            1018..>1351
                     /locus_tag="PK683_17965"
     CDS             1018..>1351
                     /locus_tag="PK683_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNH10392.1"
                     /translation="MVLGITEVKKGMVLKVEGELYSVIRSEFVNPGKGSAFIRTKLKN
                     VMKGSSIERTFKAAEKLESVELEKRSMTYCYSDGDSIIFMDVHDYEQLPVPIEYVEDI
                     LPFMKEETQ"
BASE COUNT          389 a          334 c          257 g          371 t
ORIGIN      
        1 gctttttcat aattttacgg catagctcag gttcattttt atcacaatca acaaccttgc
       61 cggaaaaaag aagatccgtg cattcaccga ctgaagaata tctgagggtc ttgagcggat
      121 tcccttcgat cagacggaaa taatccattt tgcattttcc ggaactgact ccattcttaa
      181 attctgtttc tgagcggtag gagacagaac ctacataata atgaacacct gggccattca
      241 tacagtcacc ggatgtacat aaccgccttc ttttccagat accttccacc ttcttctgtc
      301 ccggcagaac aacccatcct ttgcccttcg gggcatcatc tccatcaaag gtgcctccgt
      361 atgcagcacc gttcggataa atgattttcc cttctcctat cagtctattg tacccgtgct
      421 tccagattcc ccaattcccc tcgtacacaa gaccttccgg ttccagacgg taaaacccag
      481 gctcagggca tttgtttccg ctgtacgtaa actttccata tcctttcggc tctccgtttt
      541 caactccgcc ttcatactga tcacagtatc tagtggacag ttttgctgtc ccggaactgc
      601 agtccccgct ttcgcatttc caataacctc tgcatctgac tgcctgaaaa agcatcacaa
      661 acaataataa tctgagtaca aacatccatt cctcctgaag aacaagatct gttgtcttat
      721 ttggcttcca attggtaaat ttgccgtccc ttgaaaaagt atcggcagag acgtatcaat
      781 tcccaaaaaa actgaaacgt caactaaaaa tcttgcagcc acccttctga gcaaattatc
      841 ctgattcgac cgaatgtttc agagaaacaa agcctgtccc cttccctcag acgctcaatc
      901 cggcgctttt tagcttgacg gggagactgc aagggaaagt tctatccata atcggctgaa
      961 ttttcggtga ttcaccctca ctgagcacaa gcctcatcaa tatcacagag cggtaatatg
     1021 gtacttggaa ttacagaagt aaaaaaggga atggttctga aggtagaggg agaactctac
     1081 tcagtcatca gatcggaatt tgtgaatcct ggaaaaggaa gcgcgtttat ccgtacaaaa
     1141 ttgaaaaatg tcatgaaagg ttcatccatt gaaagaactt tcaaggcagc ggaaaaactc
     1201 gaaagtgttg aactggaaaa aagaagtatg acttactgtt attccgacgg tgacagcatc
     1261 atttttatgg atgttcatga ctatgaacag cttcctgtac cgattgaata tgtagaagat
     1321 atcctgccgt ttatgaaaga agaaacccag g
//
LOCUS       DAOQGJ010001375         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78178_length_1350_cov_2.857143, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001375 DAOQGJ010000000
VERSION     DAOQGJ010001375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78178_length_1350_cov_2.857143"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..335
                     /locus_tag="PK683_17970"
     CDS             168..335
                     /locus_tag="PK683_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10393.1"
                     /translation="MSESTKKIIKYPEWTYTVIMNLFYPGVTFYCIFPEHFPFGPAET
                     VLSRYGVFAVG"
     gene            394..648
                     /locus_tag="PK683_17975"
     CDS             394..648
                     /locus_tag="PK683_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10394.1"
                     /translation="MSVDQLRNSPVFKDVPEAPQKPISLSFWYNLLITACLDYSQIFL
                     LDRRSSYIFPDCPEYWGFLIERKRADLALKYNANKETADA"
     gene            complement(787..>1350)
                     /locus_tag="PK683_17980"
     CDS             complement(787..>1350)
                     /locus_tag="PK683_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNH10395.1"
                     /translation="IYSGKAYANIKINTDVQVFTVRPNSQEIGTAAGAGAVEKVSAGA
                     GDVKAKITGTELSGGSKIQLTEAGIIVSGGRGMKGPENFKILQDLCDTIGAALGASRA
                     AVDAGWISHSHQVGQTGKTVSPNCYIACGISGSIQHMAGMGSSKYIVAVNKDPDAPIF
                     KVATFGIVADLFDVVPAVTKEFKRVLG"
BASE COUNT          386 a          286 c          288 g          390 t
ORIGIN      
        1 attcattccg cctgaggatt atgccataaa tacaatttca ttctcagaaa acggattcat
       61 ctgctgtctt gtccgttatg aaggaggcat cagacagaag gaaggcagtg catttattgt
      121 actggcttta ctggagtaat cgaggaaaaa gaaatacagg agacgagatg tctgagagca
      181 caaaaaaaat tataaaatat cctgaatgga catataccgt catcatgaac ctgttctatc
      241 cgggagtgac attctactgc atatttccag aacattttcc tttcggccct gcagaaactg
      301 ttctcagccg gtatggggta tttgcagtcg gatgagcttt ttttgcagct gccctttcat
      361 ccgtaaattt ctttacatta aaaactatgc aaggtgtctg tcgatcagct tagaaacagt
      421 cctgttttta aggatgttcc ggaggctcct caaaaaccga tatctctgtc attctggtac
      481 aatctgctga ttacagcttg tctggattat tctcaaatct ttttattgga caggcggagc
      541 agttatattt tccctgactg tcctgaatat tggggatttc tgattgaaag aaaaagggca
      601 gatcttgcat taaaatataa tgcaaataag gaaactgctg atgcttaatt ggaaaaatac
      661 agagagatgt ctgagaagaa aaggaggaga aaagagactc agtaaaggca agtctcagaa
      721 gtcggaaaag tatcctgaga atcccgattt ccaatgacct gcggcccttg gaaatcgggg
      781 aacagattaa cctaatactc ttttgaattc ttttgtaact gcaggaacta catcaaagag
      841 atctgcaacg attccgaaag tagccacttt gaagatagga gcatccggat ccttgttgac
      901 cgcaacgata tatttggaag accccattcc tgccatatgc tgaatggaac cggaaatacc
      961 gcatgcaatg taacagttag gagatacagt ttttcctgtc tgtcctacct gatgactgtg
     1021 agaaatccag cctgcatcca cagcagctct tgaagcaccc agagccgcac cgattgtatc
     1081 gcagagatcc tgaaggattt tgaagttttc tggtcctttc atccctcttc cgccggatac
     1141 aatgatccct gcttcagtga gctggatttt gcttccgcct gagagttcag ttccagtgat
     1201 ctttgctttt acatcacctg caccggcaga aactttttca actgcacctg cacctgctgc
     1261 agttccaatt tcctgggaat tcggtcttac agtaaatacc tgaacatcag tattgatttt
     1321 gatgttcgca taagcttttc cggagtagat
//
LOCUS       DAOQGJ010001376         1349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78276_length_1349_cov_2.967543, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001376 DAOQGJ010000000
VERSION     DAOQGJ010001376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78276_length_1349_cov_2.967543"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PK683_17985"
     CDS             complement(<1..787)
                     /locus_tag="PK683_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10396.1"
                     /translation="MKKHLIYTNHILKILLLTSLVFCSRGKKDNKGIFLFLLSEQNRL
                     SKIYWDMQSSGVSSDLQSIIFQSGKYYCAGKNGTLLISSDGKSWNRQNLDTDISINKI
                     AYLGGQFFVLGENGLIMISADGQNWKNFSLITKHSLKSIATDGSTFILGGLNPLGKSV
                     VLASRTPETRTSWVESSSVSYSSSIDIAFFLNGKISMWGSTGVYTYCTSDCSTSSGWP
                     GFFRDLTQMNNNVIFDHYVRYNNTVYLASANSGKIYSTANFTDI"
     gene            complement(903..1193)
                     /locus_tag="PK683_17990"
     CDS             complement(903..1193)
                     /locus_tag="PK683_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10397.1"
                     /translation="MNKILLNIGIYFGYVIICSLIFFGLSKANDGGFAVYPMIASAFF
                     LGLHLLGTLALSISKFQDKALFKEYIISFLLLLLIGLPGCFFNFLLLISPVN"
BASE COUNT          416 a          268 c          257 g          408 t
ORIGIN      
        1 taatgtctgt aaagttcgca gtggaataaa ttttcccgga atttgccgat gcaagatata
       61 ctgtattatt atatcgtaca taatgatcaa atattacgtt attgttcatt tgagtcagat
      121 cgcggaaaaa accgggccag cctgaacttg tactgcagtc ggaagtacag taggtataaa
      181 ctccagtgct tccccacatt gagatttttc cgttaaggaa aaatgctatg tcaatgctgc
      241 tggaataact gactgacgag gattcaaccc aggatgttct ggtttcagga gtcctgcttg
      301 ccaaaaccac tgatttgccc aaagggttca agcctcctag aataaatgtg cttccgtccg
      361 ttgcgatgga tttcagactg tgttttgtaa tcaggctgaa atttttccag ttctgtccgt
      421 ctgcggaaat cattatcaaa ccattctcgc ctaacacaaa aaactgaccg ccaagataag
      481 caattttatt aatgctgatg tccgtatcca gattctgtct gttccaggat tttccgtcag
      541 aggaaatcag cagggtccca ttttttcctg cacaataata tttgcctgac tggaaaataa
      601 tggattggag atctgaactg acaccggacg actgcatatc ccagtaaatt tttgaaagcc
      661 tgttctgttc agaaagcaga aaaagaaata ttcctttatt gtcctttttt cctctgctgc
      721 agaagacaag acttgtaaga aggagtattt tcagtatatg attggtataa ataagatgtt
      781 ttttcatttt actgccttgc ctgtcaaaaa ttcttccggg atctgagaaa tcctttaaag
      841 aaccggtatg attattattc ccgcaaactg cagaaagtca agattttatt tcaagtctga
      901 actcagttga caggactgat cagaagcagg aaattaaaga aacatccagg aagtcctatc
      961 agaagcagca gcagaaatga tattatatat tctttgaaca aagctttgtc ctgaaatttt
     1021 gaaatgctca gagcaagtgt tcccaaaaga tgaagcccaa ggaaaaaagc agatgcaatc
     1081 attgggtaaa ctgcaaaacc tccatcattg gccttgctca gtccaaaaaa aatcaggctg
     1141 cagatgatta cataaccgaa atatattccg atattcagaa ggattttatt catattttct
     1201 atccacctag tcaaattctg tcagtccaga agattgctct ttctgatatg ttcgtattct
     1261 tccatcgaca taaacagatt gactttttcg cctagttctg tatgtgaaag aatcagtccc
     1321 agctgggact gattctttca catacagaa
//
LOCUS       DAOQGJ010001377         1349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78294_length_1349_cov_2.659969, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001377 DAOQGJ010000000
VERSION     DAOQGJ010001377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78294_length_1349_cov_2.659969"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /gene="rpsO"
                     /locus_tag="PK683_17995"
     CDS             <1..167
                     /gene="rpsO"
                     /locus_tag="PK683_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNH10398.1"
                     /translation="INDLNLHFSVHKKDHLSKRGLLKLVSQRKRLLSYLKRKDLNSYR
                     NLIQKLGLRK"
     gene            177..>1349
                     /locus_tag="PK683_18000"
     CDS             177..>1349
                     /locus_tag="PK683_18000"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNH10399.1"
                     /translation="MPFEKTAVIADESVSIETGKWAKQADGSIVYRVGNLVMLATVCA
                     ADESKEGQDFFPLTVEYIEKMYSVGKFPGGYIKRESKPSEHEILVSRLIDRPIRPMFP
                     DGYFCEVQLVVQVLSSDKQQSVLGHALNAASAALMVSDIPFSGPLAAVRVGRVDGKLI
                     VNPSNDLISSSDMDIIVAGSAESIMMIEGEAKELSNHELLDALKFGHGYIKEFIHLQN
                     ALAKDVAKPKREVKLKTISKELIIEVRDLCLDRMSAANRNKDKLQRGKEIGLICDEAV
                     AHFTAKDFSPEQIKDVKNYLHELEAEVVRDLIFKEGIRYDGRTSEEIRAISCEINVLP
                     GAHGSAVFTRGQTQSLGVMTLGTIADNQRYENLEGAKEKTFMLHYNFPPFSVGEIKK"
BASE COUNT          441 a          267 c          314 g          327 t
ORIGIN      
        1 gaatcaatga tctgaatctg catttcagcg tgcacaaaaa ggaccatctt tccaaaagag
       61 gtcttctgaa acttgtaagt caaagaaaaa gacttctgag ctatctcaaa agaaaagatc
      121 tcaactctta tagaaatctg atccagaaac tgggcctgag aaagtaaggc gggcacatgc
      181 cattcgaaaa aactgctgtc attgcagatg aatctgtatc tatagaaact gggaaatggg
      241 cgaaacaggc tgacggaagc attgtttacc gagtaggaaa cttagtgatg cttgccacag
      301 tctgtgctgc ggacgagagt aaagaaggac aggatttttt tcctctcact gtagagtata
      361 ttgaaaaaat gtattcagtg ggaaaatttc cgggcggtta tataaaacgg gaaagcaaac
      421 cttctgagca cgaaatactc gtatccaggc ttatagacag accgatacga ccaatgtttc
      481 ctgacggtta tttctgtgaa gtgcagctgg tagttcaagt cctttcctca gataaacagc
      541 agtcagtttt gggtcacgca ctcaatgctg ccagtgcagc actgatggtt tcagatatcc
      601 ccttctccgg acctcttgca gcagtaagag tgggcagagt tgacggaaaa ctcatagtga
      661 atccatcgaa cgatctgatc agctcttctg atatggacat tattgtggct ggaagtgctg
      721 aatccattat gatgattgaa ggtgaggcaa aagaactttc caatcatgaa cttctggatg
      781 cgttgaaatt cggtcacggt tacataaaag aattcatcca tctgcagaat gcacttgcaa
      841 aggatgtcgc aaaaccgaaa agagaagtaa aactgaagac aatcagtaaa gagctgatca
      901 tcgaagttcg cgatctctgc ctggacagaa tgtctgctgc aaacagaaac aaggacaaac
      961 tgcagagagg aaaagaaatt ggtctgatct gcgatgaggc tgtggctcac tttactgcaa
     1021 aagatttctc accggaacag atcaaggatg tcaaaaacta tctgcacgaa ctggaagcag
     1081 aagttgtgag agatttgata ttcaaggaag gaatccggta cgacggaaga acctcagaag
     1141 aaatcagagc catttcctgt gaaatcaatg tccttcctgg tgcacacggt tcggctgtat
     1201 ttacaagagg acagactcag tccctcggcg taatgacgct cggaacaatt gcagacaacc
     1261 agagatacga aaacctggaa ggtgcgaaag aaaaaacatt tatgcttcat tataatttcc
     1321 ctcctttttc agtaggcgaa attaaaaag
//
LOCUS       DAOQGJ010001378         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78489_length_1347_cov_3.232198, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001378 DAOQGJ010000000
VERSION     DAOQGJ010001378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78489_length_1347_cov_3.232198"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PK683_18005"
     CDS             <1..705
                     /locus_tag="PK683_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNH10400.1"
                     /translation="TDSLLNLPDIIVLPEAFALGFTMRSEKFAEEEDGETESFLMELS
                     VKTGSAVVGGLYLKNRNGKPWNTVSAAAEGKIICRYKKNYPFSFGGEDRFFSAGTERA
                     GFDWKGFRISLAICFDLRFPELFRGTAGETDLYIVSANWPSVRIHHWLSLLRARAVEN
                     QAYLAGVNRTGNSGGFKKIHYNGYTGIFYPSGYEKTVQSEKEEVLETVISKKELYEYR
                     ESLPFLKDIRKDRILQ"
     gene            complement(910..>1347)
                     /locus_tag="PK683_18010"
     CDS             complement(910..>1347)
                     /locus_tag="PK683_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10401.1"
                     /translation="GFSVIADVLLPVSQPDLGKVSGNMKFDSDRAGSYTGRTYIELND
                     MESKYTYRLSRASLGISYDVNEKLTIGAGFRAEKTDVSYNGLKSNPIRIASGGTGTNF
                     SAYPSPTILFNEKFLDTSLYKQNLSSDQKGVFISASYKFDLSK"
BASE COUNT          373 a          271 c          307 g          396 t
ORIGIN      
        1 acagattctc ttttgaatct gcctgatatc attgttctgc cggaagcatt cgccttggga
       61 tttacaatgc ggtctgaaaa gtttgcagag gaggaggatg gagagacgga aagttttctg
      121 atggagcttt ccgtgaaaac aggaagtgct gttgtaggcg gtctgtatct gaaaaacaga
      181 aacggaaaac cctggaatac tgtttcagct gctgcagaag gtaagatcat ctgcagatac
      241 aaaaaaaact atcctttttc attcggcggt gaagaccgtt ttttttctgc aggcacagag
      301 agagccggat ttgactggaa gggattccga atcagtcttg caatctgctt tgatctcaga
      361 tttcctgaac ttttccgggg gactgctgga gaaacggatc tgtatattgt gtctgccaac
      421 tggccctctg tcaggattca tcattggctt tctcttcttc gtgcaagggc agtcgaaaat
      481 caggcttacc ttgcaggagt gaacagaaca ggaaattccg gaggatttaa aaaaattcat
      541 tataacggat atacaggcat tttttatcct tccggatacg agaaaactgt tcagtccgaa
      601 aaagaggaag ttctggaaac tgtaatttcc aagaaagaac tttatgaata cagagaatca
      661 cttcctttcc tgaaggatat acgaaaggac agaatacttc agtgaatggc ctggtttccg
      721 gttcatttaa gacattgttc tgcataagca ggctcctgct ggcttcgctg gcctgctgca
      781 taaattttga aaacagcaaa gctggcagag actgaaaagc ggctgttttt actgacagtt
      841 gtggactgtc ctgcaggatt tctttaatgt tccttagcag attccaggca taatattttc
      901 aagtctgcac tatttagata aatcaaactt gtaggacgca gaaataaaga cacctttctg
      961 atctgagctg agattctgtt tatacaggga agtgtccagg aatttttcat taaaaagtat
     1021 cgtcggtgaa gggtaggctg aaaaatttgt tccggttccg ccgctggcta ttcgaatcgg
     1081 attgctcttc agtccattgt agcttacatc tgttttctcc gctctgaatc ccgcccctat
     1141 tgtcagtttt tcattcacat cataggatat gccgagagat gctcttgaca gtctgtaggt
     1201 gtactttgat tccatatcgt tcagttcaat atatgtcctg cctgtatagg aacctgccct
     1261 gtcagaatca aatttcatat ttcctgaaac cttccccaga tccggctgag atacaggaag
     1321 aaggacatca gcaatgacag aaaatcc
//
LOCUS       DAOQGJ010001379         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78619_length_1346_cov_2.700232, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001379 DAOQGJ010000000
VERSION     DAOQGJ010001379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78619_length_1346_cov_2.700232"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PK683_18015"
     CDS             <1..1308
                     /locus_tag="PK683_18015"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH10402.1"
                     /translation="YSVDEHTLLILRELDRLLEGTFEHRNIQSVFEECSSIHILALTI
                     LLHDVGKVKLGDHSQYGAELSRAVGERLGLSEEEILLLVFLVEEHILMSELSTKRDIT
                     DPALIREFAARTGNMDRLRLLFVLTIIDTKSVGRAVLTNWKLAILTSLYQKTADFLQN
                     GNADELGSDEEGLENVLLEKENIDPELVRHITKFASAFEPEAYIRYYTPRRIYHHFRN
                     EIHWRMEGKKDLILETEKEPAYVTMTAYYPDHKKDFLCSLAGTISAHALNLIGLRSFH
                     SDRGFTILSAQITDSTGGNSIREEQLDSLKENLKRVYLGEITVEEILSYPREWMSFNT
                     IPESMYEKKAEFDNDLHPEYTVLEVQLPDSLGLLYRILKTISETDFQLYFVRISTSAD
                     YAYDSFYLKNSRGKKIYDLKLLDSIRGKILRVGEKEIEIKAVL"
BASE COUNT          421 a          272 c          291 g          362 t
ORIGIN      
        1 tattcagtcg atgaacatac acttctcatt ctgagggagc ttgaccgcct tctggaaggg
       61 acttttgagc acaggaacat tcagtcagtt ttcgaggagt gcagcagcat tcatattctt
      121 gcactgacta ttctgcttca tgatgttgga aaggtaaaac tcggggatca ttctcagtac
      181 ggtgcagagc tttcaagagc agtcggagaa agactcggac tttctgagga agagattctg
      241 cttctcgtgt ttcttgtgga agagcatatt ctgatgtccg agctttccac caagagagac
      301 attactgacc cagccctcat aagagagttt gcggcaagaa caggaaacat ggacaggctt
      361 cggcttctgt ttgtgcttac catcattgac actaaatcag taggaagagc agttctcaca
      421 aactggaaac tggcaatact gacttctctt tatcagaaga ctgctgattt ccttcagaac
      481 ggaaacgcgg atgaactcgg ttcggatgaa gaaggacttg aaaatgttct tctggaaaaa
      541 gaaaatattg atcctgaact tgtcagacac attaccaagt ttgcgtctgc ctttgaaccg
      601 gaagcatata tcagatatta tacacccaga agaatttatc atcatttccg aaatgaaatc
      661 cactggagaa tggaaggcaa aaaggatctc attctggaaa cagaaaaaga accagcctat
      721 gtgacgatga ccgcctatta ccctgatcat aaaaaggatt ttctctgcag tctggccgga
      781 acaatttcag cacacgccct gaatctgatc ggtctcaggt cctttcattc cgacagaggt
      841 tttactatcc tgtccgccca gattactgac agtacaggag gaaattccat acgggaagaa
      901 cagcttgatt cactgaagga aaatctgaaa agagtttacc taggcgaaat tacagtagaa
      961 gaaattcttt cctatccaag agaatggatg agttttaata caattcctga aagtatgtac
     1021 gaaaaaaagg cagagtttga caatgatctg caccctgaat acactgttct ggaagtgcag
     1081 cttccagatt ctctcggact tttatacaga attttgaaga caatttctga aacagacttc
     1141 cagctgtatt ttgtgagaat ttctaccagt gccgattatg cgtacgattc tttttatttg
     1201 aaaaattcga ggggaaaaaa aatctatgat ttaaagcttc tcgattccat tagggggaaa
     1261 attctgcgtg tcggcgaaaa agaaatcgaa atcaaagcag tcctctgaaa ctgaacacag
     1321 agaaaataaa gaccagcttt cctggt
//
LOCUS       DAOQGJ010001380         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78638_length_1346_cov_2.223857, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001380 DAOQGJ010000000
VERSION     DAOQGJ010001380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78638_length_1346_cov_2.223857"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK683_18020"
     CDS             <1..198
                     /locus_tag="PK683_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10403.1"
                     /translation="AGSWKKAAEFFADSEGFSLRKKKEILNKGLSRSDLLGMKSFADK
                     ISERPIAGTGTLRFVLNRMAG"
     gene            195..>1346
                     /locus_tag="PK683_18025"
     CDS             195..>1346
                     /locus_tag="PK683_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10404.1"
                     /translation="MKKDIMYLESPWKRIHAFQGLIQKAEKNIILQMYLFSGNGELAT
                     LKPVENAFPWAHTVARLLSEKKKNFPNMNILVILDTQTVENPERTFKKKYPLARHIME
                     EAGISVVSASLKETFYNEKRSFPESAAFHRNWSNERKVNLFSKQEWAYRQNVWQTLQN
                     TEDHRKNIVIDSGKAGILFSHNIIDQAYVWHENTFLLRGKHSLDLWRLALDSAENAFS
                     LPVAFSEEDIRIKEWILQEKAEFKLHKNNDDKKNYFSEDRNGNRILSSGPEIHGEILN
                     EIRNAEKNEVTEILAASAYFSDPLTLNELRKAGEKCKVRILTDNCHALPLNFILRVIL
                     RNTVNLFCIDSCRRSERVTLRLFPSSPKEMMHCKPIAFIGRERTLIAGQA"
BASE COUNT          458 a          255 c          285 g          348 t
ORIGIN      
        1 gccggttcct ggaaaaaagc tgctgaattt tttgccgact ctgaaggttt ttcactccgg
       61 aaaaaaaagg aaattctgaa caaaggtctt tcaaggtcgg atcttctggg aatgaaatct
      121 tttgctgaca aaatttcaga gagaccgatt gccggcaccg gaactctccg attcgtgctg
      181 aacagaatgg ctggatgaaa aaagatataa tgtatctcga atcaccctgg aaaagaattc
      241 acgctttcca gggactgatt cagaaagcag agaaaaacat tatactgcag atgtatcttt
      301 tttccggaaa cggggaactt gctacactca agcctgtaga aaacgctttt ccctgggctc
      361 atacagtcgc acgtctgctt tcggaaaaaa agaaaaattt cccgaatatg aatattcttg
      421 tcattctgga cactcagaca gtggaaaatc cggaaagaac attcaaaaaa aaataccctc
      481 ttgcaagaca tattatggaa gaggctggaa tctccgtagt aagtgcaagt ctgaaagaaa
      541 cattttataa tgaaaaaaga agttttcctg agtcagcagc ttttcacaga aactggtcaa
      601 atgaacggaa ggtaaacctg ttttcaaaac aggaatgggc atacagacag aatgtatggc
      661 agacacttca aaatacggaa gatcacagaa agaatattgt cattgattca ggaaaggctg
      721 gcatactgtt ttctcataat atcattgatc aggcttatgt atggcacgag aatacctttc
      781 ttctgagagg aaaacattct cttgatcttt ggcggcttgc actcgattct gcggaaaatg
      841 cattttctct cccagtcgct ttcagtgaag aagatattcg gataaaagaa tggatattgc
      901 aggaaaaagc tgaattcaaa cttcataaga ataatgatga taaaaaaaac tatttttccg
      961 aagacaggaa cggaaacaga attctttctt caggccctga aattcatgga gaaatactga
     1021 atgaaatcag gaatgctgaa aaaaatgaag tcacggaaat ccttgcagcg tctgcctatt
     1081 tctccgatcc tctgacactc aatgaactga gaaaggcagg agagaaatgc aaagtgagga
     1141 ttctcactga caactgtcat gctttgccgt taaattttat actaagagta attttaagaa
     1201 atacagtaaa cctgttctgc attgacagct gcagaaggtc agaacgagtg acgctcaggc
     1261 tttttccatc ttctcctaaa gagatgatgc actgcaagcc aattgctttt atcggaaggg
     1321 aaagaacact gatcgcagga caggca
//
LOCUS       DAOQGJ010001381         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78693_length_1345_cov_3.872868, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001381 DAOQGJ010000000
VERSION     DAOQGJ010001381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78693_length_1345_cov_3.872868"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PK683_18030"
     CDS             complement(<1..273)
                     /locus_tag="PK683_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002724748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNH10405.1"
                     /translation="MSRTFIMIKPDAVRNRHVGEIITRIEKEGFKILALRYLQLSVDD
                     AKQFYKVHAGRPFYEELCKDMSAGPIVAAALERDNAVAHWRDVIGAT"
     gene            complement(277..774)
                     /gene="coaD"
                     /locus_tag="PK683_18035"
     CDS             complement(277..774)
                     /gene="coaD"
                     /locus_tag="PK683_18035"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNH10406.1"
                     /translation="MRIAVYPGSFDPLTKGHVDIINRALIIFDKIIIGVAVNSKKQGL
                     FSIEERIELIQKVLGSNPNVETASFSGLTADFCKEKGARAIIRGLRAVTDFDYEYAIS
                     LMNKSLSPDVETVFFMASGENSYISSSIVKEVAARHGRQVLNHVPEAVNDALMKKYQE
                     SKQKG"
     gene            complement(771..>1345)
                     /locus_tag="PK683_18040"
     CDS             complement(771..>1345)
                     /locus_tag="PK683_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNH10407.1"
                     /translation="EKAPDRPTYLELDFEKGNCAAIDGKKMNPLEVMEYLNKVGGENG
                     IGRVDIVENRLVGIKSRGVYETPGGTILHIAHRDLESMTIDRDTQHMKDDLSGEFARL
                     IYNGHWFSSKMKSMRAFIADTQEYVTGTVRVKLYKGNCTLVGRKSSFSLYSSALATFE
                     KDELYNQKDAEGFINVYGLPARETSRLRKK"
BASE COUNT          310 a          337 c          277 g          421 t
ORIGIN      
        1 agttgcaccg attacgtctc tccaatgagc aactgcattg tctctctcaa gagctgctgc
       61 tacgatcgga cctgcagaca tatccttgca gagttcttca tagaaaggtc ttccagcgtg
      121 cactttgtaa aactgcttcg catcgtccac gctcagctgc agataacgca gtgcgagaat
      181 tttaaaccct tctttttcga ttctagtgat gatttcaccg acgtgtctgt ttcttacagc
      241 atccggcttg atcatgatga atgttcttga catttcttac cccttttgtt ttgattcctg
      301 atattttttc attaaagcat cattcactgc ctcaggcaca tgattgagca cctgtcttcc
      361 gtgtcttgct gcaacttcct tgacaatgct tgaggagatg tatgaatttt cccccgaagc
      421 catgaagaat actgtctcca catcgggtga aagacttttg ttcatcagag aaattgcata
      481 ttcgtagtca aagtcagtga ctgcacggag accccgaatg attgcacggg ctcctttctc
      541 cttgcagaag tcggcagtga gcccgctgaa ggaagctgtt tccacattcg gattggagcc
      601 gagcactttc tggatcagct cgattctttc ctcaatggaa aaaagtccct gcttcttgga
      661 attgactgcc acaccgatga taatcttatc gaatataata agcgcacggt taataatatc
      721 cacatgaccc tttgtcagag gatcaaagga tccgggatat acggcaatcc tcattttttc
      781 ctgagccttg atgtttccct tgcaggaagt ccgtagacat tgatgaaacc ctctgcatcc
      841 ttctggttgt agagttcatc tttttcgaat gtggcaagcg cagaactgta cagggaaaag
      901 gaggactttc ttccgaccag cgtgcagttt cctttgtaga gtttcactct tactgtcccc
      961 gtaacatatt cctgagtgtc tgcaatgaat gcacgcattg atttcatctt gcttgaaaac
     1021 cagtgtccgt tgtagatcag tcttgcgaat tcaccggaaa gatcatcctt catatgctga
     1081 gtgtccctgt cgatcgtcat tgattcgaga tccctgtgtg caatatgcag gattgtcccg
     1141 ccgggagttt catagacccc tctggacttg attcccacga gtctgttctc gactatgtcc
     1201 actcttccga ttccgttttc accgcctacc ttgttcagat attccattac ctcaagagga
     1261 ttcattttct ttccgtcgat tgcagcacag ttcccttttt caaaatccag ttccagatac
     1321 gtcggtctgt caggagcttt ttcag
//
LOCUS       DAOQGJ010001382         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78698_length_1345_cov_3.477519, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001382 DAOQGJ010000000
VERSION     DAOQGJ010001382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78698_length_1345_cov_3.477519"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PK683_18045"
     CDS             complement(<1..972)
                     /locus_tag="PK683_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10408.1"
                     /translation="MASDSISPVVIYKSQKLGIFMDKEMTTECALTVNGTVQISSVSL
                     GIDKREIFFAPSSSGWPVNLESISYLNFSNCKDASGATVANSGSGAPVYIADGVIFLD
                     GRNGLDTNYTGATSGDPLYSLNAALSAVTSKCTGACAVLMKGGVYPILASVNMPTNVS
                     LFGGYDPSDWKKRRADKTLLSPFDTIIDDLSVNVTGTGVSPYSSIKFINYIGTKDKTV
                     LDGIAVYSPFTVNASSYSAPLGAVNLQSGAGITLRNVYTLDRSSTINVTSAGFVSSNS
                     SGSIIIKNSSLNGSTSTAASSSRYGMVYNGSNAGSVLDIYASSLSAGA"
BASE COUNT          403 a          283 c          280 g          379 t
ORIGIN      
        1 tgcacctgcc gacagtgatg atgcatagat atccagaaca gaacctgcat tggatccgtt
       61 gtaaaccatc ccatacctgg aactcgatgc ggcagtactt gttgaaccgt tcaaactgct
      121 gttctttata attatgcttc cggaactgtt cgaagaaaca aacccggcag acgttacatt
      181 gattgtactg cttctgtcca gtgtatatac atttctcaga gtaattcctg cccccgactg
      241 aagatttacc gcacctaaag gagcagaata actggacgca ttcaccgtaa atggactgta
      301 gacagcaatc ccgtccagaa cagttttatc ttttgtccca atataattta taaattttat
      361 gctcgaataa ggacttactc cggttccagt tacattcaca gagagatcgt ctatgattgt
      421 atcaaaggga gacagaagtg tcttgtctgc tcttcttttt ttccagtcag agggatcata
      481 tcctccaaaa agactgacgt tggtcggcat gttgaccgac gccaggatcg gatagacgcc
      541 tcctttcata aggacagcac aggctcctgt gcatttggat gtgactgcag aaagtgcggc
      601 atttaaggaa tagagaggat cgccggaagt cgcacctgtg tagtttgtat ccagaccgtt
      661 tcttccatcc agaaaaataa ccccgtctgc gatatacacc ggtgcaccgc ttcctgaatt
      721 cgcaactgtc gcaccggaag catctttaca attggaaaaa ttcagatagc ttatgctctc
      781 gagattgaca ggccagccgg aagaggaagg tgcaaagaaa atctctcttt tatcgatgcc
      841 gagagaaaca ctggagatct gaacggtacc atttactgta agtgcacatt ctgtcgtcat
      901 ctccttatcc ataaaaattc cgagtttctg agatttatat atgacaacag gagatatgga
      961 atctgatgcc ataaaatctg ttgtgacaaa ttttgcagga ttactgccgg ctaaagcagc
     1021 ggcaataatt accccgaaga tatttgaata ttgtgcagcc gatttactga gattcggttt
     1081 acagtaagaa aaaagaaaaa tcaggacaga tatacataac attctcagca taggaatata
     1141 ttaatgagaa aaagcaggaa aagcaatctc tttttatgtg gaaatctttg cagtattcag
     1201 gaatatatgt ttttcctttg accgtcattc aataaattga tactggattt gtaactgctc
     1261 agcctgacgg ccaatgcgtg catcgtagtt aaaaatatct gttttttcag gaatttgctt
     1321 ttaaaaaagc cagaaaaggt tgtcc
//
LOCUS       DAOQGJ010001383         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78712_length_1345_cov_3.022481, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001383 DAOQGJ010000000
VERSION     DAOQGJ010001383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78712_length_1345_cov_3.022481"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK683_18050"
     CDS             complement(<1..213)
                     /locus_tag="PK683_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10409.1"
                     /translation="MNSSFYCRIFYNYIKLYIFFISLSFLFCGKQENSSGHQTSSLQK
                     SGTASSKTHINLATLEWLPYVGEELEN"
     gene            complement(253..>1345)
                     /locus_tag="PK683_18055"
     CDS             complement(253..>1345)
                     /locus_tag="PK683_18055"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH10410.1"
                     /translation="IVFINADAMGKSLQGAGGILVFSSAVNSILSRNRASVSFQNIFP
                     ETWLKNLFIDIHHVFETFNGSMLISGITGLIDEENGFMYWMNAEHPNMVLYRDGTARF
                     LESEEAYMKFGTEGVNRVMMVSTFHLAPGDVIISGSDGRDDIGLGLDESGHAVINSDE
                     SLFLGHVETAKANLDLIYDQLKAFGEITDDLSLLKIGYSEMIDSTVKDASYLSRLEQA
                     KRFYTKKAFEQAEKLLREAYRDFPKYKDSLRLLLRILYEEKRFAEIVTLIEDYVNACP
                     WDLRFILLGAVAFAKTGNYKMGIAYSERYKLRDPHSVKNLFILYELYFNDGNYEKALP
                     VLDRILELEPLNERALSLKGGEYNDEFGV"
BASE COUNT          383 a          304 c          263 g          395 t
ORIGIN      
        1 gttttcgagt tcttctccca catagggaag ccattccaga gtcgccagat tgatatgagt
       61 tttagaggaa gcagttccgg atttctgcag agaagaagtc tgatgcccac tggaattctc
      121 ctgctttccg cagaaaagaa aagacagcga tatgaagaat atatacagct ttatataatt
      181 ataaaatatt ctgcaataga atgaagagtt catgctttct ccttctgaag actaatgaaa
      241 gaatcatgaa gattatactc cgaattcatc attgtattct cctcccttca gcgaaagcgc
      301 ccgttcattc agcggttcca gttccagaat tctgtccaga acaggaagag ccttttcata
      361 attgccgtca ttgaaataca gttcatagag aataaaaaga tttttgaccg agtgaggatc
      421 tctgagcttg tatctctctg aatatgcaat ccccatctta tagtttcctg ttttcgcaaa
      481 tgcaactgca ccaagaagta tgaaacgcag atcccaggga caggcattta catagtcttc
      541 aatcagtgtt acaatttcag caaatctttt ttcttcatag agaattcgga gaagaagtct
      601 gagtgaatct ttgtactttg gaaaatctct gtatgcttcc ctcagaagtt tctccgcctg
      661 ttcaaatgct tttttagtgt aaaaacgttt tgcctgctcc agcctgctga gataagaggc
      721 atccttgaca gtagagtcaa tcatctcaga atagccgatt ttcagaagag aaaggtcatc
      781 tgtgatttct ccaaaagcct tgagctgatc gtaaatcaga tccagattgg cttttgccgt
      841 ttctacatga cccaggaaca gtgattcgtc tgaattgatc actgcatgtc ctgattcatc
      901 cagacccaga cctatatcat cccttccgtc ggaaccggag ataatgacat cccccggagc
      961 aaggtgaaag gtactgacca tcatcacccg gttgacaccc tctgtgccga atttcatata
     1021 agcttcttcc gactcaagaa atctggctgt cccgtctctg taaagtacca tattcggatg
     1081 ttctgcattc atccagtaca tgaatccgtt ttcctcgtca atcagtcccg tgatgcctga
     1141 gatcagcatt gaaccgttga atgtctcaaa aacatgatga atgtcgatga acagattctt
     1201 cagccaggtt tcaggaaaaa tattctgaaa gctgaccgag gctctgtttc tgctgagaat
     1261 cgaattgact gcggaactga ataccaggat tcctcctgct ccctgaagag attttcccat
     1321 tgcatctgca tttataaata caatg
//
LOCUS       DAOQGJ010001384         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78722_length_1345_cov_2.737209, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001384 DAOQGJ010000000
VERSION     DAOQGJ010001384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78722_length_1345_cov_2.737209"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PK683_18060"
     CDS             <1..679
                     /locus_tag="PK683_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10411.1"
                     /translation="EGEEIFQRDMKLSLGAAVMQTKNYVPTVLYVPEYAPGTTQVVQM
                     INGQGSNPDRGTIDSTTGFGNYNVQNNVTTPGRPKFGMVGHTWDATFTWKGIYLSGAY
                     SKFRGAASNNTWGWHTTVGYNIPVSKYYIMPVLKYEHIQGDWNRNGHIDPSDHLTTYW
                     AGLNFFGDKHHFKAQLYYQVLGNKFAVDPNTGNAMAIDDRRVYLQFQANFFAGTVSPE
                     AYSYRQN"
     gene            690..1163
                     /locus_tag="PK683_18065"
     CDS             690..1163
                     /locus_tag="PK683_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10412.1"
                     /translation="MKYTFTIIIALTAILVSDCSSRKPNPNTGLRTLVLNNWLNGTAT
                     DKNKSEIILTKTDMTSYTGQCFDTFTLNGISISPSNYYNTFAGGSGGAFNSGLKRWAL
                     SASDCTSLGFTGGTGTNFGTAAQRPDPNSNFTFKMYTCDPSSSPCSKSAITAAGF"
BASE COUNT          417 a          280 c          291 g          357 t
ORIGIN      
        1 ggagggtgaa gaaattttcc agagagatat gaaactgtct ttaggggctg ctgttatgca
       61 gacaaagaac tatgttccta ctgtactcta tgttccggag tacgctcctg gtacgacaca
      121 ggtcgttcag atgataaacg ggcagggatc aaatcctgac agaggaacaa tagattcaac
      181 aaccggtttc ggaaattaca atgtgcagaa caatgtcact acccctggaa gaccgaagtt
      241 cgggatggtc ggtcatacct gggatgcaac atttacctgg aaaggaattt atctttctgg
      301 agcctattca aaattcagag gagctgcttc caacaatacc tggggctggc atacgactgt
      361 gggatataat atacctgtaa gtaaatatta tataatgcct gttctgaaat atgaacatat
      421 tcagggtgac tggaacagga acggtcatat tgatccaagt gatcatctga ccacttactg
      481 ggcgggccta aatttcttcg gagacaagca ccatttcaag gcacagcttt attatcaggt
      541 gctggggaac aaattcgccg ttgatccaaa cactggaaat gcaatggcaa ttgacgacag
      601 aagagtttat ctgcagtttc aggcgaattt ctttgcagga acagtatcac ctgaagcata
      661 cagctacaga cagaactaag gagaaagaaa tgaaatacac atttactata atcattgcac
      721 ttactgcaat tctggtttcg gactgcagca gcagaaaacc taatccgaat acggggctga
      781 gaactcttgt tttgaacaac tggctgaacg gaacagctac ggataaaaac aagtcagaaa
      841 tcattcttac gaaaacagat atgacctctt atacaggaca atgttttgat actttcactt
      901 tgaacgggat atcaatctca ccttccaatt attacaatac atttgcggga ggttcagggg
      961 gagcttttaa ttcggggttg aagagatggg cattgtctgc atcagactgt acctctctcg
     1021 gatttacggg aggcacaggc acaaactttg gtactgctgc acaaaggccg gatcctaaca
     1081 gtaattttac attcaaaatg tatacctgtg atccgagcag cagcccctgt tcaaaatcgg
     1141 caattactgc tgccggtttt taattttcaa atgcctcacg ccatcaggga ttcagaaacc
     1201 tgatggcaat attctgttta aaaaaaaata atatcaagta actgaacttt tcttacaaaa
     1261 ctcagtgccc agcttacagt catcgatcat tcagcattgg tccaacgccg acagatctcc
     1321 ttgctgtttt cgatttctaa aagca
//
LOCUS       DAOQGJ010001385         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78816_length_1344_cov_3.204810, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001385 DAOQGJ010000000
VERSION     DAOQGJ010001385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78816_length_1344_cov_3.204810"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1057
                     /gene="rfaD"
                     /locus_tag="PK683_18070"
     CDS             92..1057
                     /gene="rfaD"
                     /locus_tag="PK683_18070"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNH10413.1"
                     /translation="MKHKYIITGGAGLIGSAIVSLLNERGETDILIVDHLGSSEKWKN
                     LRRLHFRDYLEKDSFRKRVIEKPSQFQFSRFIHMGACSSTTEKDASYLIDNNYRYTLE
                     MAEFALQKNAKFIYASSAATYGLGENGYTEDNFRELQPLNMYGYSKQMFDLYADSAGI
                     LKSITGLKYFNVFGFGEAHKADMRSVVLKGYEQIKETGILKLFKSYVPEYKDGEQKRD
                     FLYVLDAAKITLHLLEGGHCGLYNVGRGVAETWTDLARALFKAMGKKENIEFVDMPDY
                     LRPKYQYYTCADMKKLSSTGYSGGFMKLDDAVSDYVSRLTSAGEN"
     gene            complement(1060..>1344)
                     /locus_tag="PK683_18075"
     CDS             complement(1060..>1344)
                     /locus_tag="PK683_18075"
                     /inference="COORDINATES: protein motif:HMM:NF025197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HNH10414.1"
                     /translation="QSIAFSKSTGKHGSASGQSKREDADSAAVSKCGATDCKTIIWAK
                     NACASLAVGSKKGFGHAWHEKKERAEQDALKNCRKYDKECEVVSTACDEE"
BASE COUNT          380 a          271 c          293 g          400 t
ORIGIN      
        1 gattgtggtt ctggctgtcg cgggttcaat ccccgtcgct ctccccactt tttcatctga
       61 cactcagtac agtgagtttt aagacaaatc catgaaacac aaatacatca ttacaggcgg
      121 agcaggctta atcggttctg caattgtttc tcttttgaat gaaagagggg aaactgatat
      181 tctgatcgtt gatcatttgg gaagttctga aaaatggaaa aatttaagaa gacttcattt
      241 cagggattat ctggaaaagg acagtttcag aaagagggtc attgaaaaac cttcgcagtt
      301 tcaattcagc agattcattc atatgggtgc atgttccagt acaactgaaa aagatgcatc
      361 ttacctgatc gacaataatt accgctatac tctggaaatg gcagaatttg ctctccagaa
      421 aaatgctaaa tttatatatg cctccagcgc agcaacgtac ggtcttggag aaaacgggta
      481 caccgaggac aatttcagag aattgcagcc tttgaacatg tacggatatt ccaagcagat
      541 gtttgatttg tatgcagatt cagccggcat tctaaaatcc atcactggac tgaagtattt
      601 taatgtattc ggtttcggtg aggctcacaa ggctgatatg agaagcgttg ttctgaaagg
      661 atatgaacag attaaagaaa caggaatatt gaaacttttc aaatcgtatg tacctgagta
      721 caaggacggt gagcagaaaa gagattttct ttatgttctg gatgcagcaa agatcactct
      781 gcatcttctc gaaggcggtc actgcgggct ttacaatgta ggcaggggag ttgcagagac
      841 ctggactgat ctggcacgtg cacttttcaa ggcaatgggg aaaaaggaaa atatcgagtt
      901 tgtagatatg cctgattatc taaggcccaa gtatcagtac tatacatgtg cggatatgaa
      961 aaagctttca tccaccggtt attccggcgg atttatgaaa cttgatgatg cagtcagtga
     1021 ctatgtttcg agactcactt ctgcaggcga aaactgaggc tattcctcat cacaggcagt
     1081 agataccacc tcacattcct tgtcatattt cctgcagttt tttaatgcat cctgttctgc
     1141 tctttctttt ttctcatgcc aggcatgacc aaatcctttt ttagatccga cagccagact
     1201 ggcacatgca ttttttgccc atattattgt tttgcagtcc gtagcaccgc atttggaaac
     1261 tgctgctgaa tctgcatctt ctcttttgct ctgaccgctt gcagatccgt gttttccagt
     1321 tgatttagaa aatgcaatcg actg
//
LOCUS       DAOQGJ010001386         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78821_length_1344_cov_3.033359, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001386 DAOQGJ010000000
VERSION     DAOQGJ010001386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78821_length_1344_cov_3.033359"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PK683_18080"
     CDS             <1..771
                     /locus_tag="PK683_18080"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNH10415.1"
                     /translation="ASIIYPVFSDPVQYSGSAILFTTYFFGLHLFLDFSGLTDMARGL
                     GKLLGLELPENFKAPFFMNSFGDFWRRWHLTFSFWIRDYIYIPLGGSKAGRFRTYFNL
                     VITFALGGLWHGANFNYAFWGIINGLFLVGERVFEENGWKLIPSVFPGPILKYLIVIH
                     ISMISWILFFTPNIQSAWSAVYRILSFQPGRDLMYAETGIYAGLFTLLFHFEQEFPER
                     FEFLQKWKPAVLPFLALFILLGLVYMSGRNMDFFYGKF"
     gene            758..>1344
                     /locus_tag="PK683_18085"
     CDS             758..>1344
                     /locus_tag="PK683_18085"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNH10416.1"
                     /translation="MANSESRFYLFYPLIFLTVLFALDKLIPADYFRKDFLQSGNRVY
                     YIQREDQLEKLKKENIGDRKFILTLGDSRAYSYSDQSFEGERKKKYILYNFSAPQAVV
                     SYHLYMLRKILASGKKPDLLFVVLSPEGFDDDRRAVYKPFLKYASDEEFREKYSELIP
                     EADKRQMLLDRIFVIRSIELDYKLLFERLQKKSLR"
BASE COUNT          339 a          273 c          287 g          445 t
ORIGIN      
        1 gcttcgatca tatatccggt cttcagtgat ccggttcagt attccggttc tgcaattctt
       61 ttcactacat actttttcgg tcttcatctt ttccttgatt tttcaggtct gactgatatg
      121 gcaagaggcc ttggcaaact tttaggcctt gagcttcctg aaaacttcaa ggctccattt
      181 ttcatgaaca gtttcggcga tttctggaga agatggcacc tgactttttc cttctggatc
      241 agagattata tttatattcc tctgggagga tcgaaggcgg gcaggttcag gacatatttc
      301 aatcttgtca ttacctttgc cttaggcgga ctttggcatg gtgcaaattt taattacgct
      361 ttctggggca taattaacgg tctttttctt gtaggggaaa gagtctttga agaaaacgga
      421 tggaaactga ttccctctgt ttttcctggg cctatcctga aatatctgat tgtaattcat
      481 atttccatga tcagctggat tcttttcttt actccgaata ttcagtctgc atggtcggcc
      541 gtctacagga tactttcctt tcagcctgga agggatctga tgtatgcaga aactggaatt
      601 tatgccggcc ttttcactct tctttttcat ttcgaacagg agtttcctga acggtttgaa
      661 tttctgcaga aatggaaacc cgctgttctt ccttttttgg cgctttttat tctgctcggc
      721 cttgtatata tgagcggacg aaatatggac tttttctatg gcaaattctg aaagcagatt
      781 ctatcttttt taccctctca tttttctgac cgttctgttt gcgcttgaca agcttatccc
      841 cgcagattat ttcagaaaag attttctgca gagcggaaac agggtttatt atatccagag
      901 ggaagatcag ctggaaaaac tgaaaaagga aaatatcgga gacaggaaat ttattctgac
      961 tctgggggac tctcgggcct attcctactc cgatcagtcc tttgaaggcg agaggaagaa
     1021 aaaatatata ctttataatt tttctgcacc tcaggcagtc gtttcctatc acctttatat
     1081 gcttcggaaa attcttgcat ccggaaaaaa accggatctg cttttcgttg tcttaagtcc
     1141 ggaaggattt gatgatgaca gacgtgcggt gtacaaaccc tttctgaagt acgcatccga
     1201 tgaggaattc agggagaaat acagtgagct tataccggaa gcggacaaaa ggcagatgct
     1261 tctagacaga atctttgtca tcaggagcat tgaactcgat tataagcttc ttttcgaaag
     1321 acttcagaaa aaatcgctca gaga
//
LOCUS       DAOQGJ010001387         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78926_length_1343_cov_2.865683, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001387 DAOQGJ010000000
VERSION     DAOQGJ010001387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78926_length_1343_cov_2.865683"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK683_18090"
     CDS             <1..314
                     /locus_tag="PK683_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10417.1"
                     /translation="GAKKRRVNTIGNLFDYSMALFDSMRHCSDYDFLVKHPDYNRLIG
                     YIDTSGHNWLNFKMTLEEKRVLFRKGVIAAYDFLEKFNWNEYKEIREKLAHSGHNGFL
                     K"
     gene            327..1274
                     /locus_tag="PK683_18095"
     CDS             327..1274
                     /locus_tag="PK683_18095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNH10418.1"
                     /translation="MKASFITAYGGLDVIQFGELPDPAQGDAQIIVQIKAVSLNPIDY
                     KIRDGNMKFVSGFKFPKILGGDFAGTVLDAGKSGGSFKNGDRVYGYIMPLFGNSGVLS
                     EKISVNPGQIRPIPENLSFEEAASLPVAALTALNGLRKFGNIQGRKILINGVTGGVGH
                     FALQAAKAKGASVTGTCSTPNTELAQKLGADSIIDYKKEDLSKTDKRFDFVFDAFGQM
                     KKEELFNVLNRNGISCTTLFFPTTMIFSLYAKIFQGISVTAANMRGKPEDWKEMEELL
                     IKGQMKPLIENTFPLERTKDAFDLMENGKPRGKIIIKVS"
BASE COUNT          433 a          252 c          309 g          349 t
ORIGIN      
        1 tcggggccaa aaaaagaaga gtgaatacaa tcggaaatct gtttgattat tcaatggctc
       61 tttttgattc aatgagacac tgctccgact atgatttcct tgtaaaacat cctgactaca
      121 acagactgat cggatatata gacacaagcg gacacaactg gcttaacttc aaaatgacat
      181 tggaagaaaa gagagttctt ttcagaaagg gtgtcattgc agcctatgat tttcttgaga
      241 agtttaactg gaatgaatac aaggaaatca gggaaaagct ggctcactct ggacacaatg
      301 gatttttaaa ataaggatac aatttaatga aagcatcatt tatcacagct tatggcggac
      361 tggacgtgat tcagttcgga gaacttcccg atccggctca gggggatgca cagatcattg
      421 ttcagatcaa agcagtttca ctcaatccga tcgattataa aattcgggat ggaaatatga
      481 aatttgtctc gggattcaag tttccaaaaa tacttggggg agattttgca ggaacagttc
      541 tggatgcagg caaatcagga ggaagtttta agaatggaga cagagtctac ggatatatta
      601 tgcctctgtt cggaaacagc ggtgttcttt ccgaaaaaat ttcagtcaat ccaggtcaaa
      661 ttcgtcccat tcctgaaaat ctaagctttg aagaagcggc ctcccttcct gtagcagcac
      721 tgacggccct gaacggactt agaaaattcg gaaatattca gggcaggaaa atacttatca
      781 acggagttac gggaggagtc ggtcattttg ctcttcaggc agcgaaggca aaaggagcat
      841 ctgtcaccgg aacctgcagc actccaaata cggagctggc gcagaaactc ggagcagata
      901 gtataattga ttataaaaag gaggatcttt cgaaaactga caaaagattt gattttgttt
      961 ttgatgcgtt cggacagatg aagaaggaag agctgtttaa tgttctgaac agaaacggaa
     1021 tcagctgcac tactcttttc tttccgacta caatgatatt ttcattatac gcgaaaatct
     1081 tccagggcat cagtgttaca gctgctaata tgagagggaa gccggaagac tggaaagaaa
     1141 tggaagaact tctcatcaag ggtcagatga agccgctcat agaaaacaca ttccctctcg
     1201 aaagaacaaa ggatgcattt gatcttatgg agaatggaaa acctagggga aaaatcatca
     1261 ttaaagtcag ttgacctttt tcccgaaaat ccggtctctg aaagtcttca agtgattttt
     1321 cattgcctcg taggcaggcc ggc
//
LOCUS       DAOQGJ010001388         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78927_length_1343_cov_2.849379, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001388 DAOQGJ010000000
VERSION     DAOQGJ010001388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78927_length_1343_cov_2.849379"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1017)
                     /locus_tag="PK683_18100"
     CDS             complement(82..1017)
                     /locus_tag="PK683_18100"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNH10419.1"
                     /translation="MWSNLLIFHSEEKNRAELNLPGFLIWQTCMRTLYIGDSRLISEE
                     AVYPRLGYQVHTGYDAYSLLLEIASGIRSRLLGETEVMAQFKETFKNENLPLTALGEY
                     ARTLRDQIIEDARKIRSSHLRNLGDQSYGGIAKKLLKNRTVTVVGSGQLAEKVIPYLA
                     EKGRTVYIYARNRERIQYLMNRFPSCRTLEGDELPETEAYVLCAPVPFEKMISGREES
                     LQIIDFREKNFGEIYSGENIEYHSFENILNSLRETENRLKILRKDLQIIVSKIAQDRE
                     FGINLNSVYCWEDIPCIAQSELHPEKVRLPGYSLT"
BASE COUNT          369 a          292 c          257 g          425 t
ORIGIN      
        1 cctgatcttt gtctccgaca gtctcacgga agtagtattc aatcggcttt tcctgacgga
       61 tccgcctgat tgctctggca actaggtaag actgtatcct ggcaagcgga ctttttctgg
      121 atgcaattct gattgagcta tacaaggaat atcttcccaa caatacactg aatttaggtt
      181 aattccaaac tctctatctt gggctatttt tgaaactatt atttgcaaat ctttacgtaa
      241 aattttcagt ctgttttccg tttctctaag agagttcagt atattttcaa aactgtggta
      301 ttcaatgttc tctccggagt aaatttcacc aaaatttttc tctctgaaat caataatctg
      361 aagagattct tctcttcctg aaatcatttt ctcaaaaggc acaggggcgc agagaacata
      421 tgcttctgtt tcaggcagtt cgtccccttc cagtgttctg caggacggaa atctgttcat
      481 cagatactga atgcgttctc tgtttctggc ataaatatag actgttctgc ccttttctgc
      541 aaggtacgga atgacttttt ctgcaagctg accgcttccc acaacggtga ctgtcctgtt
      601 tttcaaaagt ttctttgcaa ttccgccgta agactgatcg cctaagttcc tgaggtggga
      661 cgatctgatt tttctggcat cttcaataat ctgatcccga agagtccttg catattctcc
      721 aagtgccgtt aagggaaggt tttcattttt gaaagtctct ttgaactgag ccattacctc
      781 agtttctcca agaagcctgg atctgattcc gcttgcaatt tccagaagaa gtgaataggc
      841 atcatatcct gtatgcacct ggtacccaag tcttggataa actgcttctt cggaaatgag
      901 tctggaatca ccaatgtaaa gagttctcat acaagtctgc cagatcagaa atccgggcag
      961 attcaattcc gctctgttct tttcttcaga atgaaaaatt agtagattcg accacatcct
     1021 gaaattacca tactctctat tcaaaagagg attgtcacgg ttttgtcagc acgataatgt
     1081 ttttttaaaa ggaagaaaag gatgagacgc ataatcagtc tccttcaggc attcttcggc
     1141 agcccaaact gctgatgaaa acaatctgat tcgcctctgc agaaatttcc tttcagaaaa
     1201 caaaaactgc actgttttca tctgatccct tcaaaaatca tcttggaatt gcaatcatca
     1261 gtttttcacc tttgatgatg aaatacgcga gctgaaaagg agaagtcaga atatcggcgg
     1321 caaaggcgaa tgggtaaaga atg
//
LOCUS       DAOQGJ010001389         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78931_length_1343_cov_2.829193, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001389 DAOQGJ010000000
VERSION     DAOQGJ010001389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78931_length_1343_cov_2.829193"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..797
                     /gene="serB"
                     /locus_tag="PK683_18105"
     CDS             3..797
                     /gene="serB"
                     /locus_tag="PK683_18105"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNH10420.1"
                     /translation="MSESILRRNSVRFSSMYGVSSAVFSAGSENIYEIRKKFSELRAD
                     IVSVKSFFRRERKTLFSFDMDSTLIEQEVIDELAGYAGVFDEVAKVTEEAMQGKLDFR
                     EALEKRCAFLSGLSADVFDSLYSKLVMNEGVPEFLSEIRKYNSVTAVFSGGFVPILEK
                     FCRDYGITEFRANSLETVSGKLTGKVLGEIVDRDRKKKSLIELREKYGILNSDTAACG
                     DGSNDAEMIKEAGIGLGFHAKEGLKKNIQNWMDYVPLTGLLHLYEN"
     gene            797..>1343
                     /locus_tag="PK683_18110"
     CDS             797..>1343
                     /locus_tag="PK683_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10421.1"
                     /translation="MIKKLIKSAFFISLFLSCSSLEKVPVQKETEFRSPLSRNRPEKE
                     IPGFGIYVFQTDAGDSENERISAVLSELHRIWKKAGGRQWTGSPGEKFPENTAVLKTE
                     FETGENEITVIQKPLNSDSAGRIEFGFRRRKKTGFYAYIHDKIIKNNEQEKFEAFELF
                     RMPGTEVLKSILRNASQGTLSV"
BASE COUNT          413 a          238 c          322 g          370 t
ORIGIN      
        1 atatatcaga aagcattctt agaagaaact cagtgcgttt cagcagcatg tacggagtga
       61 gctctgcagt tttttccgca ggctcagaga atatttatga aatcaggaaa aagttttcag
      121 aactgagggc tgatatagtc tctgtcaagt catttttcag aagggaaaga aagacacttt
      181 tttcatttga tatggattcc actctgatcg agcaggaagt catcgatgaa cttgcaggct
      241 atgccggcgt ttttgatgaa gtggcaaaag tgactgaaga ggctatgcag gggaagctgg
      301 atttcagaga agctctcgaa aaaagatgtg ctttcctctc tggtctttct gcggatgtgt
      361 tcgattctct ttacagtaaa cttgtcatga atgaaggtgt tccggaattt ctttcagaaa
      421 taaggaaata caattcggtg acggctgttt tttcgggggg ctttgttcct attctcgaaa
      481 agttctgcag ggattacgga atcactgaat tcagggcgaa cagccttgaa acagtttccg
      541 gaaagctgac aggaaaagtt ttaggcgaga tcgtggacag agacagaaag aaaaaaagtc
      601 tgatagaact tcgtgaaaaa tacggtattt tgaattctga tactgctgcc tgcggtgacg
      661 gttcaaatga tgctgaaatg atcaaggaag ccggcatcgg gttaggattt catgcaaagg
      721 aaggcctgaa gaaaaacatt cagaactgga tggattatgt gcctcttacc ggactcctgc
      781 atttatatga aaattaatga taaaaaaatt aataaaatca gcctttttta tcagcctttt
      841 tctgtcatgt tcatctctcg aaaaggtccc tgttcagaag gagactgaat ttcgcagccc
      901 tctcagccga aaccgtcctg aaaaggaaat acccggtttc ggtatttatg tatttcagac
      961 agacgccggg gattctgaaa atgagagaat ttctgcagtt ctttcagaac ttcaccggat
     1021 ctggaagaaa gcaggcggga ggcagtggac tggttctccc ggcgaaaaat ttccggaaaa
     1081 taccgctgtg ctcaaaacag aatttgagac tggtgaaaat gaaattacag tgatccagaa
     1141 acccctgaat tccgattctg caggcagaat tgaatttgga ttcaggagaa ggaaaaaaac
     1201 cggattttac gcatatattc atgataagat cattaaaaat aatgaacagg aaaaatttga
     1261 ggcttttgag cttttcagaa tgccgggtac tgaagttctg aaaagtattc tccgaaatgc
     1321 atctcaggga acgctgtctg ttc
//
LOCUS       DAOQGJ010001390         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_78975_length_1343_cov_2.098602, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001390 DAOQGJ010000000
VERSION     DAOQGJ010001390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78975_length_1343_cov_2.098602"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PK683_18115"
     CDS             complement(<1..783)
                     /locus_tag="PK683_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNH10422.1"
                     /translation="MKYVQLNDYSLGKSSLKLAEKDPALLKEGEVRLKVHASTINPSD
                     LMFLKGMYGIKKKLPVAAGFEGSGTVSESKASLSVGTRVAFVSANNDGAWTESAVVPE
                     SNCLPLLDSMDMDDASMLFVNPLTSYSMVERGISFGAKAFVQTAAASALGKMIQRLCR
                     KKNIPLINIIRRKEQEDELRSLGEEYVLNSSDPKFEREFFKLSKKLEAVYLFEAVGGK
                     LATSVFELMPYGSRLLSYGALSEEPMSISPGICIFQNKSVEGY"
     gene            959..>1343
                     /locus_tag="PK683_18120"
     CDS             959..>1343
                     /locus_tag="PK683_18120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH10423.1"
                     /translation="MLKVESLSRKFADKTAVNNISFTVKPGVITGLLGPNGAGKTTTM
                     RLLTGYLEPTTGFISYKDRSLKGEELLNFKSKLGYLPESAPLYPDMLVSEYLEYMSDA
                     RNVPADKKQERIAEMTEICELATHIS"
BASE COUNT          377 a          327 c          294 g          345 t
ORIGIN      
        1 gtagccttcc acagacttgt tctgaaagat gcagattccc ggacttatcg acatcggctc
       61 ttcagaaagt gcaccgtagg aaagcagtct cgaaccgtaa ggcatcagtt caaatacgga
      121 agtcgcgagt ttccctccta ctgcctcaaa cagataaact gcctccagtt ttttggacag
      181 tttaaaaaat tccctctcga atttaggatc ggaagaattg agcacatatt cttctcccag
      241 ggaacggagt tcgtcctcct gctcttttct tctgatgata ttgatgagag gaatattctt
      301 ttttctgcag agacgctgaa tcatctttcc gagcgcgctg gctgcagcag tctgcacaaa
      361 agcctttgca ccgaaactga ttcctctctc caccatggaa taggatgtga gcgggtttac
      421 aaacaacata gatgcatcat ccatatccat cgaatcgaga agaggaaggc agttcgattc
      481 cggcacaact gctgattcag tccaggctcc gtcattgttt gcacttacaa atgcgaccct
      541 tgtgccgact gacagagacg ccttggattc agaaactgtt ccgcttcctt caaatcctgc
      601 tgcgacagga agcttctttt taattccgta cattcctttc aggaacataa gatccgaagg
      661 attgattgtg gatgcgtgca ctttcagcct gacttcacct tccttgagga gagcaggatc
      721 tttctctgca agtttcagag agctttttcc gagggaataa tcattcagct ggacatattt
      781 cataaatgct caacttaacc attaaggctt tcagttcaag ctaaaataac ttgacctgac
      841 agtttcccgc aaaatcttgg atgagggact gtccgactgt ctcttcaggc agaccaaaac
      901 ctgaaactgt catttcggac aaccaaatag aaattcctct tcgaactgga gattgagaat
      961 gctgaaagtc gaaagtctga gccgcaagtt tgcggacaaa actgcggtga acaatatttc
     1021 gttcaccgta aaacccggag taatcaccgg actgctcggg ccgaatggag caggcaagac
     1081 cacaacaatg agacttctca ccggatacct ggaacccact acgggattca tatcgtacaa
     1141 ggacaggtca ttaaagggag aagaactcct gaacttcaaa tccaaactcg gatatctgcc
     1201 tgaatcggca cccttgtatc cggatatgct ggtttccgag tatctggaat atatgtccga
     1261 tgccaggaac gtccctgcag ataaaaagca ggaacgaatt gctgagatga ctgagatctg
     1321 cgagctggca acacatatca gta
//
LOCUS       DAOQGJ010001391         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79197_length_1341_cov_2.048212, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001391 DAOQGJ010000000
VERSION     DAOQGJ010001391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79197_length_1341_cov_2.048212"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PK683_18125"
     CDS             complement(<1..1283)
                     /locus_tag="PK683_18125"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symporter"
                     /protein_id="HNH10424.1"
                     /translation="MSSAIAIIIPTLIYVYYVFIESWCLAYAVDFFSRAGQAANHGMD
                     IKQVVADSGLRFETMTGAKANGAALGSSIIWFLIFCYLLNYFIVYRGIAKGLEKLAKI
                     AAPILFICSSVLLFRVLSLDGIMNGLGKMWNPDWNALAKADVWVAAAGQIFFSLSVGF
                     GIVLVFTSYLTENDDVTLSGLTAASLNEFVEIVFGGMITIPVWFLFLAGSSSDFSTFS
                     LGFTALPAIFSQMPFGNIFAGIWFTTLFIAAITSSVTMLQPAINFLEEDFHLHRRYSS
                     AILFIFTSTLTAGIVYYSRDLLALDYTDFWVGTMLIYILATIQVIIYGWVIGPKKGRE
                     ESEKGALWSIPKTFDFVIKYITPVILILILAVFISQNLTEYINKMSPEYMQMNAAKYK
                     MTPAEAYENAVVARSVFFVILGILGMTFVMVHFGI"
BASE COUNT          419 a          309 c          282 g          331 t
ORIGIN      
        1 ctgatcccga aatgcaccat tacaaaagtc attccgagta ttcctaatat tacaaaaaat
       61 acagaccttg ccacaactgc attttcgtag gcttccgcag gggtcatttt gtattttgcc
      121 gcattcatct gcatatattc aggactcatt ttattgatgt attctgtcag attctggctg
      181 atgaaaactg caagtatcag tatcagtatg actggggtaa tatatttaat cacaaaatca
      241 aatgttttcg ggatactcca taatgcaccc ttttcactct cctcgcgtcc ctttttcggc
      301 ccgatcaccc atccgtagat gatgacctga atagtggcca ggatatagat gagcattgtc
      361 cctacccaga aatcagtgta gtcaagggcc agaagatccc tgctgtagta tactattccc
      421 gcggtaagag tcgatgtgaa aataaagagt attgcagagg aatatcttct gtgaagatga
      481 aagtcctctt cgaggaagtt gattgcaggc tggagcattg tcactgagga agtgatggct
      541 gcaatgaaaa gtgtggtaaa ccagatccct gcaaatatgt ttccgaaggg catctgactg
      601 aaaatcgcag gaagagctgt aaaacccaga ctgaatgtgg agaaatcaga actgcttcct
      661 gccagaaaca gaaaccagac aggaatggta atcattcctc cgaatacaat ttccacaaat
      721 tcgttcagag atgctgctgt cagacccgac agagtcacat catcattttc ggtaagataa
      781 ctcgtaaata caaggactat cccgaatcct acagaaagag aaaagaatat ctgacccgca
      841 gctgcaaccc agacatccgc cttcgccaga gcattccagt ccggattcca cattttaccg
      901 agaccgttca tgattccgtc cagactcaga acacggaaaa gaagcacgga agagcatata
      961 aaaagaatcg gagcggcaat ctttgcaagt ttttccagtc cctttgcaat tcctctgtac
     1021 acaatgaaat aattcagaag atagcagaat ataagaaacc agataataga ggaaccgagg
     1081 gctgcaccgt ttgcctttgc tcctgtcata gtttcaaacc gaagtccgct gtctgcgact
     1141 acctgcttaa tgtccattcc gtgattggca gcctgccctg ctctcgaaaa aaaatcgaca
     1201 gcataggcaa gacaccagga ttctatgaag acataataga catagatcag agtaggaatg
     1261 atgattgcaa tcgcactgct catccggaga gcaaaaccgt ccggcagaaa tgcagaaaaa
     1321 atcgctctgg aactgtgacc t
//
LOCUS       DAOQGJ010001392         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79433_length_1338_cov_4.377241, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001392 DAOQGJ010000000
VERSION     DAOQGJ010001392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79433_length_1338_cov_4.377241"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..>1338)
                     /locus_tag="PK683_18130"
     CDS             complement(111..>1338)
                     /locus_tag="PK683_18130"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10425.1"
                     /translation="ITLRSQSLQGKFKELIVSLFEKTNQKAVILVDEYDKPILDVIEN
                     EETAASIRDELKNLYSVIKDSDAYIKFVFITGVSKFSRVSLFSGLNNLSDITLDSRFS
                     AVCGYTQSELQNTFQDRLQGVPLNELKEWYNGYNFCGEKVYNPYDILHYLDKKDFQNY
                     WFATGTPSFLIRLIERFRYPIPNLENIRLSAEQLESFDVRSIELETILFQTGYLTVKN
                     IEKKGINTVYHLEYPNIEVKHSLNDVILKFLLKLMTEKETNKVKLYELLFSGNIAELR
                     DLFHAFFASIPYQWYTNNTISSYEGYYASVFYSYFAAAGIEVRVEDSTNLGRMDMTAV
                     LNGRCFIMEFKVNEMTSSGNALAQLRKKKYHEKYVGYSGAQISSGTVNEIYLIGIEFS
                     KMDRNITSFDWETVQL"
BASE COUNT          397 a          247 c          249 g          445 t
ORIGIN      
        1 tgaattgcag tacacacttt taagtcagca aatctcatag gaatattttt tttaaggggg
       61 gtattttgtc aagtattatt tgaggttcag caggatacac aatgggtaaa tcaaagttga
      121 actgtttccc agtcaaaaga agtgatattc ctatccattt tactgaactc gatcccaatg
      181 agataaattt catttactgt tccggaactg atctgagctc cagaatagcc gacatatttt
      241 tcgtggtatt tttttttccg aagctgcgcc agagcatttc ctgatgaagt catttcattg
      301 actttgaatt ccataataaa acatcttccg ttcagaacag ctgtcatatc cattcttccc
      361 agattggtgc tgtcttccac tctgacttca attcccgctg cagcgaagta agaataaaag
      421 acagaagcat agtatccttc ataactggaa atagtattgt ttgtatacca ctggtatgga
      481 attgatgcaa aaaaagcgtg gaacagatct ctcagttctg caatatttcc cgagaaaaga
      541 agttcataca gtttcacttt atttgtttcc ttctctgtca tcagttttaa aagaaattta
      601 agtatcacat cattgagact gtgtttaact tctatattag gatactctaa atgataaact
      661 gtattgattc ctttcttttc aatatttttg actgtcaagt agccagtctg gaaaagaata
      721 gtttcaagtt caatagaacg gacatcaaag ctttccagct gttctgccga gagacggata
      781 ttttccagat ttggaatggg gtatctgaac ctttcaatga gtctgattaa aaagcttgga
      841 gtgcctgtag caaaccagta gttctgaaaa tcttttttat ccaggtaatg caggatatca
      901 tagggattgt atactttttc tccgcagaaa ttgtaaccat tgtaccattc tttaagttca
      961 ttcagtggga cgccttgcag tctgtcctga aaagtattct gcagttcaga ctgggtataa
     1021 ccgcagactg cggaaaaacg ggaatccagt gtaatgtcgg acaaattgtt aagtccggaa
     1081 aaaagactta cgcgggagaa tttactgact ccggtgataa aaacaaattt tatgtatgca
     1141 tcagaatctt taattacgga atatagattt ttcagttcat ctctgataga agcagcagtt
     1201 tcttcatttt caattacatc aagtataggc ttgtcatatt catcaaccag tatgactgcc
     1261 ttttgattag tcttttcgaa aaggctgaca atcagttcct tgaattttcc ctgcaatgac
     1321 tggcttctca gtgtgatc
//
LOCUS       DAOQGJ010001393         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79462_length_1338_cov_3.032736, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001393 DAOQGJ010000000
VERSION     DAOQGJ010001393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79462_length_1338_cov_3.032736"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..605
                     /locus_tag="PK683_18135"
     CDS             99..605
                     /locus_tag="PK683_18135"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNH10426.1"
                     /translation="MIGQSKSHLQIFMGVMFGIIRIFVIVLIVLHSLKLRDFSILLPY
                     EGLSSLQWDFLGFSVGLIGLSFCLYAQLKMKNSWRVGIDEGSREDLVTDGLYRWIRNP
                     TYLGLFILNFGVWLIWPTYLVSYLNIIFFFLLEVQVRCEEDFLEGKYGTEYLNYRKNT
                     YRYIPFIY"
     gene            908..>1338
                     /locus_tag="PK683_18140"
     CDS             908..>1338
                     /locus_tag="PK683_18140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH10427.1"
                     /translation="MKIISLGVNSAFATGTYEETLNAAKLQDFLKTVEHKPDAEFRND
                     IQEYINSQKEISYRPKWQANFLIEFDRPSKRDGKPPYRLIVDFGGDVRHSLKGFGLNF
                     NQIDGYYVSHPHADHIGGVEGIALMTFFNPAYSQKKLEWLNG"
BASE COUNT          378 a          262 c          314 g          384 t
ORIGIN      
        1 ttacgaaaac aatctacaac gttccagatt tttgaagcac cggtttatgg aattttaaaa
       61 gagtcattca gcgaaaacag acagggatcg atcctgaggt gataggacag tcaaaatctc
      121 atctgcagat ttttatgggt gtgatgttcg ggataatcag aatttttgta attgttctga
      181 ttgtccttca ttcattaaaa ttgagggatt tctcaatact tcttccttat gaaggattat
      241 cttcattgca atgggatttt ctcggatttt ccgtcggact tattggactt tccttctgtc
      301 tgtatgcaca gctgaaaatg aaaaattcat ggagggttgg gatagatgag ggaagccgtg
      361 aggatcttgt caccgacggg ctgtaccgct ggatcagaaa tccgacctat ctcggtctgt
      421 ttatcctgaa cttcggagta tggctgatct ggcctacata ccttgtctcc tatctgaata
      481 tcatattttt ctttctgctt gaggttcagg tcaggtgtga agaagatttt ctcgaaggaa
      541 aatacggcac cgaatacttg aattacagga aaaatacata tcgttatatt ccttttatct
      601 attaacgcag gctggtgcag aggttcgcag ttatggaagg cgaggctgtc ttcccgcgtc
      661 agggatcgac ccggcgtcat ttttctgaga atgcagaaaa atgacgggag gggagagcgc
      721 gaccgacgtt cctctcagca ttggaacatt ttctgtctag ggatatggaa cgtcggtgac
      781 gcccattcag ataaaagaag attgagattg tgaattttgt atcgtcctaa gcctcggatt
      841 ccctgttcag aacgttcctg taaataaatt agcttgaaga aataaccctg aaaataagat
      901 atcctttatg aagataatca gcctaggggt caattctgct tttgccacag ggacatatga
      961 agagaccctg aatgcagcta aacttcagga ttttctgaag actgttgaac ataagccgga
     1021 tgcagagttc cgcaatgaca ttcaggagta tatcaattcc cagaaagaaa tcagctacag
     1081 gccgaagtgg caggcaaatt ttctcataga atttgacaga ccgagcaaaa gagacggtaa
     1141 accgccttac aggctgattg tagacttcgg cggagacgtc agacattctc tgaaaggatt
     1201 cggtctcaat tttaatcaga tagacggata ctatgtctct catcctcatg cagaccatat
     1261 cggcggagtg gaagggattg cgctgatgac tttcttcaac cctgcctatt ctcagaaaaa
     1321 actggaatgg ctgaacgg
//
LOCUS       DAOQGJ010001394         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79465_length_1338_cov_2.948558, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001394 DAOQGJ010000000
VERSION     DAOQGJ010001394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79465_length_1338_cov_2.948558"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PK683_18145"
     CDS             complement(<1..922)
                     /locus_tag="PK683_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10428.1"
                     /translation="MKYAFLSVLLILLQCAKAEVQKPRFYRPALTVSVQRETLEDGKK
                     KISSPITVLGSSRTVEFIYEDGFDDLAEYHLEYAVKIIPKIADYFQTAPTKEAFRITQ
                     LRDGSTDRNEGESIYFHLDNQFNKSQRIPTGHPLLFHEIGHWWFGQNPRFMAEGVSSY
                     LPVVLNESGLLQLSEKEMNDIKNWWGFYKQTVIGDVPLGDDEKKELSGEPGFSLNYEK
                     SYKIQYILHKELGAKKYKDFLLSLLDTQASDRNWVLKTQYHHDTESIFNALQNQKKTD
                     WKSLLSGWIGNAGYKKISLKDWQDSDMDGLL"
     gene            complement(1073..>1338)
                     /locus_tag="PK683_18150"
     CDS             complement(1073..>1338)
                     /locus_tag="PK683_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10429.1"
                     /translation="GKIEKDGSIRDSHGSYTGKIENDGDIRDSRGSFIGKIENDGTVR
                     DRYGSYLGRIEKDGDVRDKYGSYIGKVGPGVSKGHAAIQFFFK"
BASE COUNT          359 a          279 c          253 g          447 t
ORIGIN      
        1 ccagcagacc gtccatatcc gaatcctgcc agtctttcaa agatatcttt ttgtaaccag
       61 cgtttcctat ccagccggag agaagagatt tccagtccgt ttttttctga ttctgaagag
      121 cattaaaaat agattcagta tcatgatgat actgtgtttt caatacccag tttctgtcag
      181 acgcctgagt gtccaggaga ctgagcagga aatccttata cttttttgcc ccaagttctt
      241 tgtgaagaat atactgtatc ttgtaggatt tttcatagtt cagtgaaaac ccaggctcgc
      301 cagagagttc ttttttttca tcatcgccga gaggtacatc tccgatgact gtctgcttgt
      361 agaaacccca ccagtttttt atatcattca tttccttttc gctcagctgc agaagccctg
      421 attcattgag taccactgga agatagctgc tgactccctc agccataaat ctaggattct
      481 gtccgaacca ccagtgtccg atttcatgaa acagaagagg atggcctgtc ggaattctct
      541 gtgatttatt gaactgattg tccagatgga aataaatgga ctctccttca tttctgtctg
      601 tagaaccgtc tctcagctga gtaattctga atgcttcctt cgtcggtgca gtctgaaaat
      661 agtcagcaat tttaggaatg atttttactg catattcaag atgatattcg gcaagatcgt
      721 caaaaccgtc ttcgtatata aattctacag ttctggaaga tcccagaaca gtaataggtg
      781 aacttatttt cttttttccg tcttcaaggg tttctctttg aacagaaact gtcaaagcgg
      841 gtctgtaaaa tcggggtttt tgcacctctg cttttgcgca ttggagcaga atcagtaaaa
      901 cagagaggaa tgcatatttc atacgggatt cataaatata cggatttatt tttttgacaa
      961 gaagaatttt ggaaagtttt ctgactgtgc tgaggtttaa attattatag gattttttat
     1021 tgaataaagg actttgcaga aatcagaaac agaatgatta gaggtgaatg agtcatttaa
     1081 aaaagaactg gattgctgcg tgtcctttgg aaacacccgg accgactttt ccaatataac
     1141 ttccatattt atctctgaca tctccgtctt tttctatcct gcctagatag ctgccatatc
     1201 tgtcacgaac tgttccgtca ttttcaattt ttccaataaa acttccgcga ctgtctcgaa
     1261 tgtcgccatc gttttcaatc tttcctgtat aacttccatg agaatctcgg atggaaccgt
     1321 ctttctcaat tttacctt
//
LOCUS       DAOQGJ010001395         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79681_length_1336_cov_3.174863, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001395 DAOQGJ010000000
VERSION     DAOQGJ010001395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79681_length_1336_cov_3.174863"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PK683_18155"
     CDS             complement(<1..762)
                     /locus_tag="PK683_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10430.1"
                     /translation="MKDNSMKYIISLFFMFISLSSLSADSTFAHPLQVEPVTVYKKVR
                     VDAKGVENKESGNYDRQRNVSFEAEWSFLDMFSVKGGGGATKYESTGNKPSTLTDRWN
                     LGLKFGKAFGGESFRFGVGAGMRLFNKQTGASPYYQNAPDLYLVKPSFSLGMGFGALD
                     IVAEMSLVSETNPTFREKVKQDFRRHYVKGLSVSYGLDNNQVRLFLETLYYEPYDKRV
                     DKNIDSWYMCPGISYKLYEGGWLTASAQVPMRNELY"
     gene            complement(752..>1336)
                     /locus_tag="PK683_18160"
     CDS             complement(752..>1336)
                     /locus_tag="PK683_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH10431.1"
                     /translation="GGQTLANAVKAGLQFKVIDEAGPSIKYMLLSLGGNDLQGNPSAY
                     AGNIAAEKARRLAQIQENLLGMVSSGNFYKLQKWGGDPLTWIIYGYDYPNPDVPSVQD
                     STSCRSTLKSAGFSDTDVDTLVVSTLNEYNVFLSQMITKDYHIRYIDLRGTLGGPYSQ
                     KQYMYDCIHPNSAGFRLLGNRYAETLKGFTNNER"
BASE COUNT          366 a          335 c          250 g          385 t
ORIGIN      
        1 atacagttca tttctcatcg ggacctgagc tgaagcagtc agccaccccc cttcatacag
       61 cttataactg atgccgggac acatgtacca agaatcgata tttttatcaa cacgcttatc
      121 ataaggctca tagtacagag tttccaaaaa cagtctaacc tgattgttat ccagaccgta
      181 tgaaacagaa agccctttca cataatgtct tctgaaatcc tgcttcactt tttcccgaaa
      241 ggtcggattc gtctcgctta caagagacat ttcggccaca atatccagtg caccgaatcc
      301 cattcctaaa gagaaactcg gttttaccag ataaagatcc ggcgcattct gatagtaagg
      361 acttgctcct gtctgcttgt tgaaaagacg cattcctgct cctaccccga atcgaaaaga
      421 ttccccgccg aatgcttttc cgaatttaag tcccagattc cacctgtcag tcagtgttga
      481 cggcttgttc cctgttgatt catacttagt tgcaccgccc cctcctttca ctgaaaacat
      541 atccagaaaa ctccactcag cttcgaatga aacgtttctc tgtctgtcat aatttccgga
      601 ttccttgttc tccacaccct ttgcatccac tcttactttt ttgtagacag ttaccggctc
      661 cacctgaaga ggatgagcaa aagtgctgtc tgcactgaga ctggaaagag agatgaacat
      721 aaaaaataat gatataatat atttcataga attatctttc attgtttgtg aaccccttca
      781 gtgtctctgc atatcggttt cctagaagac ggaatcctgc agaattcgga tgaatacagt
      841 catacatgta ctgtttctga gaatacggtc ctccgagagt acctctcaga tcaatgtagc
      901 ggatatggta gtctttcgtt atcatctggg aaaggaatac attgtattca ttcagagtgc
      961 tgactacaag tgtatccacg tccgtatcgg aaaagcctgc actcttcaag gtagatcggc
     1021 aggaagttga gtcctgcacg gaaggtacat caggattcgg ataatcatag ccatatatga
     1081 tccatgtgag aggatctccg ccccatttct gcagcttgta gaaatttcct gaactaacca
     1141 tacccagaag attttcctga atctgagcaa gccttctggc cttctctgca gcaatatttc
     1201 ctgcataggc agaaggattg ccctgaagat cattacctcc taatgataaa agcatatatt
     1261 ttatacttgg ccctgcctcg tcaatcacct tgaactgaag acctgctttc actgcatttg
     1321 caagagtctg ccctcc
//
LOCUS       DAOQGJ010001396         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79782_length_1335_cov_3.166406, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001396 DAOQGJ010000000
VERSION     DAOQGJ010001396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79782_length_1335_cov_3.166406"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PK683_18165"
     CDS             complement(<1..490)
                     /locus_tag="PK683_18165"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNH10432.1"
                     /translation="MKKGFSTRAIHSGKRPDLTTGAITPPIYATSTYVQESPGRHKGY
                     EYSRSQNLTRESYEKCIADLEGGAEGFAFSSGLAAETACLELLDSGSHVIAQDDMYGG
                     TYRLMENLKKRSANLSFSFVDMSDRNAIEKAIQKNTKMIWIETPSNPLLKIVDLSMIA
                     DIA"
     gene            complement(493..>1335)
                     /locus_tag="PK683_18170"
     CDS             complement(493..>1335)
                     /locus_tag="PK683_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH10433.1"
                     /translation="EKYVFKTEEIVGKWLKARKRDSVIIASKVAGPGHGWFSPPVRSG
                     KTALDRHSIMKAVEGSLKRLGTDYIDLYQTHWPDPDMYHEETLFALTELVQSGKVRYI
                     GCSNETPWGLMKSLWASEKNRLVRYESIQNNFSMLNRRFEDSLAEICKKEGVSLIAYS
                     PLAGGVLSGKYNTNKIPENARFTKYAKLAERQKKQSSRYLNELTVGATAGFMKIAKEM
                     GISVTTLAIAWSKQNDFTASTLIGANTVDQLNDSLKAADVIIPNEILDKINEVSKNIP
                     YPMG"
BASE COUNT          322 a          305 c          270 g          438 t
ORIGIN      
        1 tggcgatatc tgcaatcatg gaaagatcca caattttcaa aagagggttg gaaggagttt
       61 ctatccatat catctttgta tttttctgta ttgctttctc tattgcattt ctatcagaca
      121 tatccacaaa gctgaaggaa agatttgctg accttttttt cagattctcc atcagtctgt
      181 atgtcccgcc gtacatatca tcctgcgcaa ttacgtgaga gcctgaatcc aaaagctcca
      241 ggcaggcagt ttctgcagca agacctgaac tgaatgcaaa cccttcggca ccgccttcca
      301 ggtcagcaat gcatttttca taagattctc tggtcagatt ctgggaacgg ctgtattcat
      361 atcctttatg tctgcctgga ctttcctgaa cataagtaga agtggcataa atgggaggag
      421 tgattgctcc tgtagtgaga tcaggtcttt tccctgagtg aattgctctt gtactgaatc
      481 cttttttcat agttacccca tcggataagg aatatttttg gatacttcat tgattttatc
      541 caagatttca ttaggaatta tcacatcagc cgctttcaga gaatcattca gctgatccac
      601 tgtatttgca ccgatcagag tggacgcagt gaaatcattc tgtttgctcc aagcaattgc
      661 cagtgtagtc accgaaattc ccatttcctt tgcaattttc ataaagcctg cagtggcacc
      721 gacagtcagt tcattcagat atcgactgga ctgtttcttc tgcctttcag caagtttagc
      781 atactttgtg aatcttgcat tttccggaat cttgtttgtg ttgtattttc cgctgagtac
      841 cccgcctgca agaggagaat atgcaatcag actgacacct tcctttttgc agatctcagc
      901 aagagaatct tcgaagcgtc tgttcagcat actgaaatta ttctgaatgc tttcatatct
      961 gaccagtctg tttttttcgg atgcccagag acttttcatc agtccccagg gtgtttcatt
     1021 gctgcatcct atgtatctga ctttgccgga ctgtacgagt tccgtcagtg caaaaagagt
     1081 ttcttcgtga tacatatccg ggtccggcca gtgggtttga tacagatcta tataatcagt
     1141 tccaagtctt ttcagactgc cttccacggc tttcatgatg ctgtgtctgt caagggctgt
     1201 cttgccggat ctcacaggag gtgaaaacca tccatggcct gggccggcta ccttggatgc
     1261 aatgatgact gaatcgcgtt ttctggcttt cagccattta cctacaattt cttcagtctt
     1321 aaaaacatat ttttc
//
LOCUS       DAOQGJ010001397         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79895_length_1334_cov_2.831900, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001397 DAOQGJ010000000
VERSION     DAOQGJ010001397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79895_length_1334_cov_2.831900"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PK683_18175"
     CDS             complement(<1..733)
                     /locus_tag="PK683_18175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH10434.1"
                     /translation="MLYRITLDTNPEDCNLHCIMCEEHSEYSSFIQRLGRKRRMPEEW
                     LERIFSEAKALGVQEIIPSTMGEPLLYRHIQKIFQLCREFGIRLNLTTNGTFPEIRGM
                     NLKKWADEIIPVCSDVKISWNGAHSRTAESVMKGLKHSKAVQNLTEFIAVRDSHYIIS
                     GHRCRITLQTTFLKSNMNELEEMIRFAASVGADRVKGHHLWAHFPEIENLSFRADNES
                     VKRWNEIVEKCLHLCEDLYKKEGKRV"
     gene            complement(831..>1334)
                     /locus_tag="PK683_18180"
     CDS             complement(831..>1334)
                     /locus_tag="PK683_18180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH10435.1"
                     /translation="IEAFIKLYAEFRKDCRLIIFGRHQGQNTDYLRRVSSGYPIEFRN
                     EYMNEEIAEKVFSETDCIVVPSVWGENSPLVIHEAQACRIPVITADYGGMKEYVNEGV
                     NGLLFQHRDSDDLCKKMKEAAANPIKMKELGQRGYLYSENGDVPDIGEHCRQLVKIYA
                     SLIQKKT"
BASE COUNT          362 a          311 c          222 g          439 t
ORIGIN      
        1 caactctttt cccctctttt ttataaagat cttcacagag atgcagacat ttttccacaa
       61 tttcattcca tcttttcacg gactcattat ctgcacgaaa tgaaagattt tctatttcag
      121 gaaaatgagc ccaaagatga tgtcccttca ctcggtctgc tccgactgag gctgcgaaac
      181 gaatcatttc ctccagttcg ttcatattag atttaaggaa agtggtctgc agggtgattc
      241 tgcatcgatg cccggatata atataatgac tgtctctgac tgcaataaat tccgtaagat
      301 tctgaaccgc cttagaatgc ttcagtcctt tcattaccga ctctgcggtc cggctatgtg
      361 caccgttcca ggatattttg acatctgaac agacaggaat aatctcatca gcccattttt
      421 tcaaattcat ccctctgatt tcaggaaagg ttccattggt tgtcaggtta agacggattc
      481 cgaattccct gcagagctga aatatttttt gtatatgcct gtataaaaga ggttcaccca
      541 ttgtggaagg aatgatttcc tgaacaccaa gagccttagc ttcagagaat attctctcaa
      601 gccattcttc cggcattctt ctctttcttc caagtctttg aataaagctg gaatattcac
      661 tgtgttcttc acacataatg caatgcagat tgcagtcttc aggatttgta tccaaagtga
      721 ttctatacag cataattata tatatttcca gttgatctgc ttatcattaa tttcaatatt
      781 ggaagacaaa gcccaacata agcacaatta gaaaacagaa ttcagccagg tcaagttttt
      841 ttctgaatta gagatgcata gattttgacg agctgcctgc aatgctctcc tatgtcaggc
      901 acatctccat tttctgaata cagataacct ctttgaccaa gctccttcat ttttatcgga
      961 ttcgctgcag cctccttcat ttttttacac aaatcatcgg aatctctgtg ctgaaacaaa
     1021 agtccattca ctccttcatt tacatattcc ttcattcccc cataatctgc agtgatgacc
     1081 ggaattcgac aggcctgtgc ttcgtgaatc acaagaggtg aattctcgcc ccagacagac
     1141 ggcacaacta tgcagtctgt ttcagaaaac accttctctg caatctcttc attcatatac
     1201 tcatttctga attctatcgg gtatcctgat gatactcttc tgagataatc cgtattctgt
     1261 ccctgatgtc ttccaaagat gatcagtctg cagtcttttc tgaattctgc atacagtttt
     1321 ataaatgctt ctat
//
LOCUS       DAOQGJ010001398         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79905_length_1334_cov_2.626271, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001398 DAOQGJ010000000
VERSION     DAOQGJ010001398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79905_length_1334_cov_2.626271"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK683_18185"
     CDS             complement(<1..387)
                     /locus_tag="PK683_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNH10436.1"
                     /translation="MSERSYYEILGVSKTASDDEIKKAYRQLAIKYHPDKNKGNKEAE
                     DKFKEATEAYEILRDSQKRRAYDQFGKAGVSGGAGFGQGATNYSDIFGGFGDIFEEFF
                     GKGGAGGGGGGRRGPRRGDDLRYDMDI"
     gene            complement(398..>1334)
                     /locus_tag="PK683_18190"
     CDS             complement(398..>1334)
                     /locus_tag="PK683_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNH10437.1"
                     /translation="ILVGGSTRIPAVQKLVKDLFGKEPNKSVNPDEVVAAGAAIQGGV
                     LGGEVTDVLLLDVTPLSLGIETLGGVMTRLIERNTTIPTRKAQVFSTAADNQTAVSVH
                     VLQGEREMARDNRTLGRFDLVGIPAAPRGVPQIEVSFDIDANGIVHVSAKDLGTGKEQ
                     KIRIESSSSLSEEEIQKMVKDAEKNAAADKLARETAEARNETESLVYALEKTVSESGD
                     KIQAGDKQLAEDEIKRAKEALASDNLERIKSAKESVSRIANKIAEELYSKGGAPSDGS
                     APNEQSAGTAGSGKTAGTGEKVVDADYTVVDEDKK"
BASE COUNT          268 a          361 c          266 g          439 t
ORIGIN      
        1 gatgtccata tcatatctca ggtcatctcc tcttctgggt ccccgtcttc cgccgccgcc
       61 tcctccagct cctccttttc cgaagaattc ttcgaatatg tctccgaatc ctccgaaaat
      121 atcggaatag tttgtagccc cctggccgaa tcctgcaccc ccgctcactc cggcttttcc
      181 gaactgatcg tatgctcttc ttttctggga atcccgaaga atttcataag cttcagtagc
      241 ttctttgaac ttgtcttcgg cttccttgtt gcctttgttt ttgtccggat ggtacttgat
      301 ggcaagctga cgataagctt tctttatctc atcgtcagat gccgtcttgg agacacccag
      361 aatttcgtag tagctccttt cgctcatagt ctcagtctta ctttttgtcc tcatcgacta
      421 ctgtatagtc agcatctaca accttctctc cggtacctgc tgtcttgcct gaacctgctg
      481 ttcctgcaga ctgttcattt ggagcagacc cgtcagaagg agcaccgccc ttgctgtaga
      541 gttcttccgc aatcttattt gcaattctgg atacagattc ctttgcagat ttgattcttt
      601 ccaggttgtc gctggcaagc gcttcttttg ctcttttgat ttcatcttct gccagctgct
      661 tgtcgcctgc ctgaatcttg tcgccggatt cggatacagt tttttccaga gcatacacaa
      721 ggctttcagt ttcatttctg gcctcagctg tttctcttgc gagtttgtca gctgcagcat
      781 tcttttctgc atccttgacc atcttctgaa tttcttcttc agaaagactg gagctggact
      841 caattctgat tttctgttct tttcctgttc cgagatcttt tgcagataca tgcacaatac
      901 cgtttgcatc tatatcaaaa gaaacttcaa tctgaggaac tcctctcggt gcagcaggga
      961 ttcccacgag atcaaatcgg ccgagagttc tgttgtctct tgccatttct ctttctccct
     1021 gcaggacatg cacggaaact gctgtctgat tgtcagctgc agtactgaat acctgtgctt
     1081 ttctggtagg gatagtagtg tttctttcaa tcagtctggt catcacaccg ccgagagttt
     1141 caattccgag agaaagagga gtgacatcca gaagaagaac atctgtcact tctccgccga
     1201 gcacgccgcc ttgaattgca gcaccggcag ccacaacctc atcaggattg actgatttgt
     1261 tcggttcttt tccgaaaaga tcttttacga gtttctgaac tgcaggaatt cttgtggaac
     1321 ctcccactaa aatc
//
LOCUS       DAOQGJ010001399         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79983_length_1333_cov_3.844288, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001399 DAOQGJ010000000
VERSION     DAOQGJ010001399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79983_length_1333_cov_3.844288"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..642
                     /locus_tag="PK683_18195"
     CDS             28..642
                     /locus_tag="PK683_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10438.1"
                     /translation="MKKSFFIIIFFLISFSTDAVDVPKDPVPPVLQDTTVEKKDGSEK
                     GKDKKKKEKRSVNISAVLCDGREVSGKIETDRDELNLSHKSDGILYEKKVSFDDLKQI
                     YIRTWKAERKKKEKDGFTFQFTPSQITVSGKSGDIWNISSLKGTDLSTLSVKNRNGTA
                     RLYSFWIDLQYDNGKWFSKLPGFSGNEREDCHPDAFRSIRFYAE"
     gene            complement(646..>1333)
                     /locus_tag="PK683_18200"
     CDS             complement(646..>1333)
                     /locus_tag="PK683_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10439.1"
                     /translation="PKNVKEEKTEVKEVKEVNAYVKAASPSASAPSSGTGNAPKEESA
                     SGLFSVGVVFSPVESGLAKESMSRYGKPTKEYLVENTFNILKEKEKTVTVQKDPNAAP
                     GAPVQEPDDIDLDDKELENKVPMALIWDLGNKTSQDGHKGGFVIQWNEPYHKKLYTKR
                     IDYFSSELSGLITTDFKKYFSARETKILTDLLSEPRFGTETRSSSEETPVKTTPSAPV
                     KETVPATTNP"
BASE COUNT          376 a          268 c          296 g          393 t
ORIGIN      
        1 ggagaataca gacggggaat gtagtatatg aaaaaatctt tctttatcat tattttcttt
       61 ctcatcagtt tttccactga tgcagtggat gttccgaagg atccggtgcc ccctgtgctt
      121 caggatacga cagtcgaaaa aaaggacgga tcggaaaaag gaaaggataa aaagaaaaaa
      181 gagaaaagat cagtcaatat atctgcagtg ctctgcgacg gaagagaagt gtccgggaaa
      241 attgaaactg accgggatga gctgaattta tctcataaat ctgacggaat tctgtatgag
      301 aagaaagtat cctttgatga tctgaaacag atctatatca ggacatggaa ggcagaaaga
      361 aagaaaaagg aaaaagacgg gtttactttt cagttcacac cgagccagat cactgtttca
      421 ggcaaatcgg gagacatatg gaatatcagt tctctgaaag gaaccgatct ttccactctt
      481 tccgtcaaaa acagaaacgg aacagcaaga ctttacagct tctggattga tcttcagtat
      541 gataatggga aatggttttc taaacttccg ggattttcag gaaatgaaag agaggactgc
      601 cacccggatg cattcagatc catccggttt tatgcagagt aaaaattaag ggtttgtcgt
      661 agcaggcact gtttctttca caggagctga aggtgttgtc ttgacagggg tttcttctga
      721 agatgatctg gtttctgttc cgaatctagg ctcgctcaga agatcagtca gaatctttgt
      781 ttctcttgca gaaaaatatt tcttaaaatc tgtcgtaatc aggcccgaca gttcagaact
      841 gaaataatca attcttttag tatacagttt cttatggtaa ggctcattcc actgaatgac
      901 aaatcctccc ttgtggccgt cctgacttgt cttgtttcca agatcccaga tcagagccat
      961 aggaactttg ttttcaagtt ccttgtcgtc gagatcaata tcatccggtt cctgaacagg
     1021 agcccccggg gccgcattcg gatctttctg aacggtgaca gttttttcct tttccttcag
     1081 aatattgaat gtgttttcca caagatattc ttttgtaggt ttcccgtatc tggacattga
     1141 ctctttcgcc agtcctgatt cgacaggact gaagactacc ccgaccgaaa aaagacctga
     1201 agcagattct tccttaggag catttcctgt tccggatgag ggtgcagaag ctgaaggtga
     1261 tgctgccttg acataggcgt tcacttcttt tacttccttc acctcggtct tctcttcttt
     1321 tacgtttttt ggt
//
LOCUS       DAOQGJ010001400         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_79989_length_1333_cov_3.569640, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001400 DAOQGJ010000000
VERSION     DAOQGJ010001400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79989_length_1333_cov_3.569640"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PK683_18205"
     CDS             <1..249
                     /locus_tag="PK683_18205"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH10440.1"
                     /translation="DVREEAEYVLENLGGVSIPLAKILQNAEKIATDRSVIIHCGSGR
                     RSANAILELQQRFGFTNLLNLKGGIQAWKEETDSNIAV"
     gene            251..>1333
                     /locus_tag="PK683_18210"
     CDS             251..>1333
                     /locus_tag="PK683_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH10441.1"
                     /translation="MHEEQEHFDTDAIRKQLKRSTNREHSVPIYMTSSFVFESAEQAR
                     ALFSDEEPGNIYTRFSNPNNTEFVEKICEMEHAEDGIATASGMAAIFSTLSSLLKSGD
                     HVVVSRAVFGSTHQIMTVLFPRWNIDHTYVDISHPEKWESAIKKNTKAIFVETPSNPG
                     LDIIDLKWLSALCRKRGLILIVDNCFSTPYLQKPILLGADIVIHSATKYIDGQGRTIA
                     GITVGRKDLIKDVRFFARHTGPALSPFNAWILSKSLETLSIRMEKHCENAYKLATYLE
                     THPEITLVKYPFLPSHPQYELAKKQMKLGGGIVTFIVKGGLERGRKFLNELEMISHSA
                     NLGDTRTIATHPASTTHSKLNAEEREA"
BASE COUNT          424 a          297 c          286 g          326 t
ORIGIN      
        1 gatgtcaggg aagaagccga atatgttctg gaaaatttgg gcggagtatc cattcctttg
       61 gcaaaaatac ttcagaatgc ggaaaaaatt gcaacagaca gatcagtcat cattcattgc
      121 ggaagcggga gaagaagtgc aaatgcaatt ttggaacttc agcagaggtt cggcttcaca
      181 aatcttctga atctgaaagg cggaattcag gcctggaaag aagaaacaga ctcaaacata
      241 gcggtatgat atgcacgaag aacaggaaca ttttgacacg gatgcgatcc gtaaacagct
      301 gaaacggagc acaaacagag aacacagcgt tccgatttac atgacatcca gtttcgtatt
      361 tgaaagtgcg gaacaggcca gagctctctt ttcagatgaa gaaccgggca atatatatac
      421 aagattctcc aacccgaaca atacggaatt tgtggaaaaa atctgcgaaa tggaacatgc
      481 tgaagacggg attgcaaccg catcgggaat ggcagcaata ttttccactc tttcttcatt
      541 attaaaatcc ggcgaccatg tggttgtatc ccgtgctgta ttcggttcca ctcatcagat
      601 catgacagtc ctgttccccc gctggaatat cgaccatact tacgtagata tttctcatcc
      661 tgaaaaatgg gaatctgcaa taaaaaagaa tacaaaagca atatttgtgg aaactccttc
      721 caatccggga ctggacatca ttgatctgaa atggctttct gctctctgca gaaaacgcgg
      781 gctcatactg attgtagaca actgtttctc cactccttat cttcagaaac cgattcttct
      841 cggagcagac atcgtaatcc actctgccac aaagtacatt gacggacagg gaagaacaat
      901 tgcagggatt acagtgggaa gaaaggatct gatcaaggat gtgcgctttt ttgcacgtca
      961 tacaggacct gctctttctc cgttcaatgc atggattctt tccaaaagtc tggaaactct
     1021 ttccatcaga atggaaaaac actgcgagaa tgcatacaaa ctcgccactt atctcgaaac
     1081 tcatcctgaa atcactctgg tcaagtatcc tttccttcct tcgcatcctc agtatgagct
     1141 tgccaaaaaa cagatgaaac tcgggggcgg aatcgtcaca ttcatcgtaa agggcggact
     1201 ggaaagagga agaaaattcc tgaatgaact ggaaatgatt tctcattctg ccaatctggg
     1261 cgataccaga acaattgcca ctcatcctgc atctacaact cattccaagc tgaatgcaga
     1321 agaaagagaa gct
//
LOCUS       DAOQGJ010001401         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80012_length_1333_cov_2.962441, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001401 DAOQGJ010000000
VERSION     DAOQGJ010001401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80012_length_1333_cov_2.962441"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1333
                     /locus_tag="PK683_18215"
     CDS             <1..>1333
                     /locus_tag="PK683_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HNH10442.1"
                     /translation="QRKTEAELEEVRKKNDEIMNRMSEDSRKLEELEQTRQEAERKIS
                     EIDRQLLDFLSQKDILKEKIEKNKSMIAEYVFRMEEQTKNLETDRAKLDRLENEKTAL
                     EDSIRSFQETEELTQGNISKQEQRKAALESELAELQNRIQTYELTVREKEKSLVILRE
                     NVKEIVVNLIQQIESKKKDAVSGESRRLELKNYLLGFLDDLILASESPDSTELFKNFS
                     FRDYRENLKNFLELEDVFRDIVFDKDGMLARKESLDSEIEELILENEQLLSDIRSLQS
                     ESEEKRKNISELKEEVYSLEKQLLESRTGRTSLDAGRAKLMENIAETKERYDSVALSL
                     TSISEKKTAYENEVVQLEEQIERSYNEFLNISKILETEKTDLKNLTSEIQNLKSSTGK
                     DQEEFKSLLPLLSELERKNSSQKTQMEAFQEELYNDYSITDQDLEQEKSGMT"
BASE COUNT          506 a          251 c          304 g          272 t
ORIGIN      
        1 cagagaaaga cagaagcgga acttgaagaa gtcaggaaaa aaaatgatga aatcatgaac
       61 cgtatgtcgg aggactccag aaaactcgaa gaactcgaac agacaagaca ggaagcagaa
      121 agaaagattt ctgaaataga cagacagctc ctggattttc tttcacagaa ggacattctg
      181 aaagaaaaga tcgaaaaaaa taaaagtatg attgcagagt atgttttcag gatggaagag
      241 cagacaaaaa atctggaaac ggacagagca aaactggaca ggctggaaaa tgaaaagact
      301 gcattggagg attccatcag aagttttcag gaaactgaag aactgactca gggaaacatc
      361 agcaagcagg aacagagaaa ggctgcactg gaatcggaac tggccgaact gcagaacagg
      421 atccagacct acgaactgac tgtcagagaa aaagaaaagt ctcttgtcat tctgcgcgaa
      481 aatgtaaagg aaatcgtagt caatctcatt cagcagatag agtccaagaa aaaagatgct
      541 gtctctggag aatccagaag actggaactg aaaaattatc ttctcggatt tctggatgat
      601 ctgattcttg cttcagaatc accggacagc actgaactct ttaaaaactt ttccttcaga
      661 gactacagag aaaatctgaa aaactttctg gagcttgagg atgtgttcag ggacatcgta
      721 tttgacaagg acgggatgct tgcgagaaaa gaatcccttg attcagaaat agaggaactc
      781 atcctggaaa atgaacagct cctttccgat atccgctctc ttcagtcaga gtctgaggaa
      841 aaaagaaaaa atatttccga actgaaggaa gaagtctaca gcctggaaaa acagcttctt
      901 gaaagcagga caggaaggac ctcactggat gcaggcagag ccaagctgat ggaaaacatc
      961 gctgaaacaa aggaaaggta cgactcggtc gctctatcac tcacttccat cagcgaaaag
     1021 aagactgcat acgaaaatga agtggtccag ctcgaagagc agattgaaag atcgtataat
     1081 gaatttttaa atatcagcaa aatcctggaa acggaaaaaa cagatcttaa gaatctcacc
     1141 tcagagattc agaatctcaa atcctccaca ggaaaggatc aggaagaatt caaaagtctt
     1201 cttcctctgc tttctgaact ggaaagaaaa aactcctccc agaaaactca gatggaagca
     1261 tttcaggaag agctgtataa tgattattcc attacagacc aggatctgga gcaggaaaaa
     1321 tcggggatga cac
//
LOCUS       DAOQGJ010001402         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80067_length_1333_cov_1.774648, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001402 DAOQGJ010000000
VERSION     DAOQGJ010001402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80067_length_1333_cov_1.774648"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PK683_18220"
     CDS             complement(<1..1054)
                     /locus_tag="PK683_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH10443.1"
                     /translation="MTRAVSKDNSEKKIWQVIFASSVGTMIEWYDFYIFGTLASFIAA
                     QFYPKGNETVSYLAALATFATGFIVRPFGAVVFGHIGDKFGRKYTFLLTMLIMGGATF
                     VIGILPGYDVIGMAAPAALVFLRLVQGLALGGEYGGAATYIAEHSPDEKRGYYTSFIQ
                     TTATLGLFVSIGVILLTRMTLSAADFNEWGWRIPFLLSAFLVLISYYIRIRLQESPVF
                     LEMKASGKASANPIRESFGNSANLKMVLIALFGATAGQGVVWYTGQFYALSFLEKTLK
                     VDYTTAYTVTAVALALATPFFIFFGSLSDRIGRKKIILTGCLLAAILYIPIYSAMSRF
                     QSNAVMLTVFVFVQVFL"
     gene            complement(1064..1315)
                     /locus_tag="PK683_18225"
     CDS             complement(1064..1315)
                     /locus_tag="PK683_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10444.1"
                     /translation="MEYSAEISYWEKYTERWDLDVMSEKLEYAKMHSYYCAFHAIFGL
                     LYLKDAEFSSHSQTLGYSNKEFIHSEILGAIQNYIQENF"
BASE COUNT          402 a          313 c          259 g          359 t
ORIGIN      
        1 ccaggaatac ctgcacaaat acaaaaacag tcagcatcac tgcgtttgac tggaacctgg
       61 acattgcaga ataaatcggg atatacagta ttgcggcaag aagacatcct gtcaatatga
      121 tttttttcct gccgatcctg tctgaaaggc ttccgaagaa tatgaaaaac ggagttgcca
      181 gtgccaacgc gaccgctgtc acagtgtagg cagtcgtata gtccaccttc agtgtttttt
      241 cgagaaaact cagtgcataa aactgaccgg tataccagac aactccctgt cctgcagtcg
      301 caccgaacag agcaatcaga accatcttga gattggcaga gttcccgaaa ctctcacgga
      361 taggattggc tgatgctttt ccggatgcct tcatttccag gaacactgga gactcctgaa
      421 gtctgattct tatataatag gaaatcaata ctaaaaatgc ggagagcaga aaaggaattc
      481 tccagcccca ttcattgaag tcagccgcac tcagtgtcat acgggtcaga agaatcacac
      541 cgatcgagac aaaaagtcca agtgttgcag tggtctggat gaagctcgta tagtaccctc
      601 ttttttcatc aggagaatgc tccgctatat atgttgctgc gccgccgtac tctcctccca
      661 gtgccagccc ctgaaccagt cggagaaata caagagctgc aggagcagcc atcccgatga
      721 catcgtatcc aggaagaata ccgatcacaa atgtggcacc tcccattatg agcatcgtta
      781 ataaaaaagt atattttctg ccgaacttat cacctatatg accgaatacc actgcaccga
      841 acggtcttac aatgaaccct gtagcaaatg tggccagagc agcaagataa ctgacagtct
      901 catttccttt cggatagaac tgtgctgcaa tgaaacttgc cagtgtcccg aatatgtaaa
      961 aatcatacca ctctatcatt gttccgacag aagacgcaaa aatcacctgc cagattttct
     1021 tctctgaatt atcttttgat actgctctgg tcataactct tccttaaaaa ttttcctgaa
     1081 tataattttg gattgcgcct aatatttccg aatgaataaa ttctttatta gagtagccta
     1141 atgtttgact gtgggaggaa aactcagcat cttttagata gagtaagccg aatatagcat
     1201 gaaaggcaca ataatatgaa tgcatttttg catattcgag tttctcgctc ataacatcca
     1261 gatcccatct ttcagtatat ttttcccaat aggaaatttc tgctgaatat tccactcttc
     1321 ttcattcaga atg
//
LOCUS       DAOQGJ010001403         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80126_length_1332_cov_2.729052, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001403 DAOQGJ010000000
VERSION     DAOQGJ010001403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80126_length_1332_cov_2.729052"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            546..779
                     /locus_tag="PK683_18230"
     CDS             546..779
                     /locus_tag="PK683_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10445.1"
                     /translation="MPGGLSQIAGVPTKLGPPRVVGVPTKPVSLRIAGVPTKLGPLRI
                     VGVPTKLGSLKIVGVPTKAGYPRIAGVLQSPSL"
BASE COUNT          390 a          304 c          288 g          350 t
ORIGIN      
        1 attagatttc cctaatgttt tccgaaaatt actgcatcta gacggaatat tgtatttgcg
       61 taaggtgagt gattgaacta aacatagctg acagtgtcca gattgtatct gaaaggtcaa
      121 aaaaaaaatc gttcactgat cttagtcatc actttctatt tcagaaactc accagatgat
      181 tctgtggatg agtctcattt attggttgaa cttccctgca gtcatcctaa tcatattttt
      241 gggacaggga gaatgacaat gcaaatctac tgcttcggga tgattttata gactggtttt
      301 tttcagacaa agaaattcgc atttttccct gcagtcgttt tctttgcatc ggctttaacc
      361 tctctcagta tatatgcaga tacagttgaa ctcagaaata aaaaaatata tgacggagtg
      421 aaagttgtca taacacagac gtcggtcctg ccggaggcat cgtttccata agaattcctc
      481 aataagacta aaagattgtc ccgggaattt gcgttggaac tacataggag ttccaacaaa
      541 acgctgtgcc aggaggtctt tcccaaatcg caggagttcc tacaaagctt ggccctccga
      601 gagttgtagg agttcctaca aaacccgtct ctctgagaat cgcaggagtt cccacaaagc
      661 ttggtccgct gagaatcgta ggagttccca caaagcttgg ttccctgaaa atcgtaggag
      721 ttcccacaaa ggccggctat ccgagaatcg caggagtcct acaaagcccg tctctctgag
      781 aatcgtagga gccccacaaa gcccgtctct ctaagattcg caggagttcc aacaaagctt
      841 ggcactctga gaatcgcagg agttcccaca acgctcggct ctctgagaat cgtaggagtt
      901 cccacaaagc ttggtcccct gaaaatcgca ggagccctac aaagtgttgt cccggaagtt
      961 cttaaccaat tgcgacagtc cctaattctt tgtaatgatg ttcctcagaa aatacctgac
     1021 cgcagaggtt cttcctatca gaattttgaa aaccaagcga gagagcactc accgcatatg
     1081 cagcaagtgt cctcggcttg gaaaaaagca acgaagtagc gttagcgact actgagctcc
     1141 gtccttaggg ctgtcgaaaa ggcccaaagg gtaatgctgt caagataagg atgattcacc
     1201 acatacacac ggattaacac agataatgaa tcaaaatatc cgtgttgatc tgtgctcatc
     1261 cgtgataatc ctttaaattt cttcttaagt tgacagcata agcccaaagg gggtcgcaag
     1321 agtggaagct gc
//
LOCUS       DAOQGJ010001404         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80230_length_1331_cov_3.144201, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001404 DAOQGJ010000000
VERSION     DAOQGJ010001404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80230_length_1331_cov_3.144201"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..>1331
                     /locus_tag="PK683_18235"
     CDS             263..>1331
                     /locus_tag="PK683_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10446.1"
                     /translation="MGHNEVISDIWNFLAYLFPFLASIAFVLFLRKIDKGDQELSEIR
                     ERKLDKFTEILDDREIQFEERFEEIEKLASETNSKIENFETLLKNRTDHSGKIVEERL
                     ADYSAKFQTRLDQIFDEANHSISLISKEMQLEAKSMREEMQIMHKPFAEREAEFLKEF
                     DSRKNRILDEFQSITKDSERNLEQKVVRIKELTDVLKDKIENLDFQFDSKWEDLNHLF
                     QDKMQSLEKRFSDRFDSVIDETKESRENYMKGFRNELQAFRNQIDDLKGYMQSRQEEI
                     LSDSKRSIELFSERILQLSDRFSDIDSRITKQAEFHKTEVGKELRNFEEEFMMFRDKS
                     MEDLEKSLSDMKEKTDRLEDDI"
BASE COUNT          474 a          216 c          289 g          352 t
ORIGIN      
        1 gaatgagcga ctttgcgaat ttatttctga aaaacattga tgaattcata tgacctcttt
       61 ttttagaccg aagaacagtc tgaaagtcaa aatatttcaa taatttcatt ccgcaattgc
      121 aattttcgga aaaatcggac tgaacttttt cccgctttct tcacaggact gcagaaaaat
      181 tttgaacacc ctttttttca ggcctgccga tgatgaaaat agggtttact gtatatttct
      241 ttttctggta tgggtttaag caatgggaca taatgaagtt atttcggaca tttggaattt
      301 tttggcgtat ctgtttcctt ttctcgcaag cattgcattt gtgcttttcc tgagaaaaat
      361 agacaaaggg gatcaggaac tgagcgaaat ccgagaaaga aaacttgata aattcacaga
      421 aattttagac gaccgtgaaa ttcagtttga ggagcggttt gaagaaatcg agaaactggc
      481 ttctgaaacg aattcaaaga tagaaaattt tgaaaccctt ctcaaaaaca ggacagacca
      541 ctccggaaaa atagtggaag aaagacttgc agattattct gcaaaatttc agacaaggct
      601 cgatcagatc tttgacgagg caaatcacag catctcactg atttcgaaag aaatgcagct
      661 tgaagcaaag tcgatgcggg aagaaatgca gattatgcat aagccctttg cagagagaga
      721 ggctgaattt ctgaaggaat ttgattccag aaaaaacaga atcctggatg aatttcagag
      781 cattacaaaa gacagtgaaa gaaatcttga gcagaaagtc gtacggatca aggaactgac
      841 cgacgttctc aaggataaaa tagaaaatct ggatttccag ttcgacagca agtgggaaga
      901 tctgaatcac ctgtttcagg acaaaatgca gtccttggaa aaaagattca gtgacagatt
      961 tgattcagtg atagatgaga caaaagaatc cagagagaac tatatgaaag gattccgaaa
     1021 tgaacttcag gcattccgaa atcagataga tgatctgaag gggtacatgc agtcgagaca
     1081 ggaagaaatt ctttcagatt ccaaaagaag cattgaactt ttttctgaga gaattcttca
     1141 gctttcagac aggttttcag acattgattc aaggataaca aaacaggcag aatttcataa
     1201 gacggaagtc ggaaaggaac tcaggaattt tgaagaagag tttatgatgt tcagagacaa
     1261 gtccatggaa gatctcgaaa aaagtctttc tgatatgaag gaaaagacgg acagactcga
     1321 agatgatatc a
//
LOCUS       DAOQGJ010001405         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80232_length_1331_cov_3.115987, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001405 DAOQGJ010000000
VERSION     DAOQGJ010001405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80232_length_1331_cov_3.115987"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PK683_18240"
     CDS             complement(<1..399)
                     /locus_tag="PK683_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNH10447.1"
                     /translation="MDLKRTLSWQDSASMMISSIVGSGIFISAGALARDVGDPRLIMI
                     CWILGGLFAAAGAMSYAFPAVIFPKAGGDYIYLKEAYSPLVAFMSGWAALLANLSANI
                     TVIALAFSSNFFDIFPEFKFELFHLNLKFLE"
     gene            523..>1331
                     /locus_tag="PK683_18245"
     CDS             523..>1331
                     /locus_tag="PK683_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10448.1"
                     /translation="MEEKKVPEAATAAAEVKDPPVSEELLVTSLFLSGRLQTVDKSVP
                     IGSYLKNDSYGVYKKKIHDHWAQYKKLIYDPLVEWRKKNIPKVDNKLVFYPFSGPDFP
                     NANALYPDAQEYIMIGLEPGGLEPNIDAMPKEKVMRGLFEIVASQDSISRLNFYMTNK
                     MKQNISASVFKGTAPVILTYFGLLGFKPLSLRSITVNDEGNIEYLTQKDIAEKYKNGN
                     VSVEFIFLDAEGRKKKLTYFSANVANEGITAKPNVMKFLEKKESFVSTFKAA"
BASE COUNT          430 a          284 c          296 g          321 t
ORIGIN      
        1 ttccagaaat ttcaggttca gatggaaaag ttcgaattta aattccggga atatgtcaaa
       61 aaaattgctg gagaacgcaa gtgcaatgac tgtaatattt gcggaaagat tggccaaaag
      121 ggctgcccag ccgctcataa atgccacgag aggggaatag gcttctttca gatagatata
      181 gtcccctccc gccttgggaa aaatgactgc aggaaaagca taactcattg cccctgctgc
      241 agcaaaaagt ccgcctaaaa tccagcagat cataatcaga cgaggatccc ctacgtccct
      301 tgccaatgca cccgccgaaa taaaaatccc ggaaccgacg atcgaactga tcatcatgga
      361 ggcagaatcc tgccaagaaa gtgttcgctt taaatccata gctttgggaa ctgtctaatc
      421 ataagcatga agttcaagaa tattttttta atactgataa ttttcctctc agtcgcagtt
      481 tcctgtaaac gggaaaagac tgacggcact gttcctgtgg ttatggaaga gaaaaaagtc
      541 cctgaagcag cgacagcggc ggcagaggtc aaggaccctc ccgtctctga agaactttta
      601 gtcacaagcc tgtttctttc cggaagactt cagacggtgg acaagtctgt ccctatcggg
      661 agctatctga agaatgattc ttacggagtc tacaagaaga aaattcacga tcattgggcg
      721 cagtacaaaa aactgatcta tgatcctctg gtggaatgga gaaagaagaa tatcccgaaa
      781 gtagataata agcttgtatt ctatccgttc tcaggtcccg actttccgaa tgcaaatgca
      841 ctgtaccccg acgcacagga atacattatg atcggtctcg aacctggcgg attggaaccg
      901 aacatagatg caatgcccaa agaaaaagtg atgagaggcc ttttcgaaat tgtagccagt
      961 caggattcca tttccaggct gaatttctat atgaccaata aaatgaagca gaacatctcc
     1021 gcttccgtat tcaaggggac tgctcctgta attctcacat acttcggact tttgggattc
     1081 aaacctctgt cgctgagaag cattaccgtc aatgatgagg gcaatataga atatctcact
     1141 cagaaggata ttgcagagaa atataaaaac gggaatgtat cagttgaatt catttttctg
     1201 gacgctgaag gaagaaaaaa gaaactgacg tatttttccg caaacgttgc aaatgaagga
     1261 atcacagcaa agccgaatgt aatgaagttt ctggaaaaaa aagaaagttt cgtcagcacg
     1321 ttcaaggcag c
//
LOCUS       DAOQGJ010001406         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80292_length_1331_cov_1.996865, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001406 DAOQGJ010000000
VERSION     DAOQGJ010001406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80292_length_1331_cov_1.996865"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1331
                     /locus_tag="PK683_18250"
     CDS             <1..>1331
                     /locus_tag="PK683_18250"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH10449.1"
                     /translation="ARLILGGFFVLGSISSLVTTPIVILIVGYFLASFIFIGYSIYGL
                     QIIQKKEIRIFHVYLFSFLDIFTLLIIRLMLAFLAPEGIDYGLNEKVIFSIPFIYIVL
                     APIRYNIRFTVFTLSLCLGQEIILQAICIPFVKILPPGQNLTPGFVDVIRLGTADLFI
                     LGCCISSYLLTTVTSRNISMIQEEESKAKERLEKNAGVLERLQSSIQTLNELKNSTHE
                     NITKLQTGSQRQAAATEETASALNAISASAQEISKAARKQADLNLGLNGITVANEEAF
                     EQIKKKLEELKLWNSKLQKITSRGQEVILKTNRSMEDMKESSIGIFKVIGVMREIAYR
                     TNLLALNASIEAARAGEQGKGFSVVAEEVGKLAQNSSIQTKEISSNIQFNLDGLKEST
                     VSMENLVKIFDETAKGIDKAVQLLDFCFESLAKFETNRKEMSFHIEEMSSV"
BASE COUNT          422 a          272 c          292 g          345 t
ORIGIN      
        1 aggcaagact gatactcggc ggtttttttg tcctaggaag catcagctca cttgtcacaa
       61 cacctattgt aattctcatt gtcgggtatt ttcttgcgag ttttattttc ataggataca
      121 gcatatacgg acttcagatc attcaaaaaa aagaaattcg gatcttccat gtttatcttt
      181 tcagtttcct ggatattttc actcttctga ttatcaggct tatgctggct tttcttgcac
      241 cggagggaat cgattacgga ttaaatgaaa aggtaatttt cagtatccct ttcatctata
      301 tagtgcttgc tcctatcaga tacaatatca ggttcactgt tttcactctt tctttatgtc
      361 tcgggcagga aatcattctg caggcaatat gcattccttt cgtaaaaatc cttcctccgg
      421 gacaaaatct tactccaggc tttgtagacg tgatcagact cggtactgcg gatctgttta
      481 tcctgggatg ctgcatttct tcctatctcc tgacgacagt cacctcaaga aatatttcca
      541 tgattcagga agaggaatcc aaggcaaagg aaagacttga aaaaaatgcg ggtgttctgg
      601 aaagacttca gtcctccatt cagaccctga atgaactgaa aaattccact catgaaaaca
      661 ttacaaaact gcagacagga agtcagagac aggccgcagc tacagaagag actgcatctg
      721 cactgaatgc catttctgca tctgcacagg aaatctcgaa agcagcgaga aaacaggcag
      781 acctgaatct tggactgaac ggaatcacag tcgcaaacga agaagccttc gaacagatca
      841 aaaagaaact ggaggagctg aaactctgga acagcaaact tcagaaaatc acttccaggg
      901 gacaggaagt gattctgaaa acgaacagat cgatggaaga tatgaaagaa tcctccatcg
      961 ggattttcaa ggtcatcggc gtaatgagag aaattgccta cagaacaaat cttctggcac
     1021 tgaatgcatc gattgaagcg gcaagggcag gagaacaggg aaagggattt tcggtcgttg
     1081 cggaagaagt aggaaaactt gcacagaatt ccagcattca gacaaaggaa atcagcagca
     1141 atattcagtt caatctggac ggactgaagg agagcactgt atccatggaa aaccttgtca
     1201 agatctttga tgagacagca aaggggatag acaaggcagt gcagcttctc gatttctgct
     1261 ttgaatcact ggcaaaattt gaaacgaaca gaaaggaaat gtcattccat atagaggaaa
     1321 tgagttctgt a
//
LOCUS       DAOQGJ010001407         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80330_length_1330_cov_3.913725, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001407 DAOQGJ010000000
VERSION     DAOQGJ010001407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80330_length_1330_cov_3.913725"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /gene="rpmB"
                     /locus_tag="PK683_18255"
     CDS             complement(<1..223)
                     /gene="rpmB"
                     /locus_tag="PK683_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017809470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNH10450.1"
                     /translation="MARKCVVTGKGTMYGNAVSHSHLKTRRIWKVNIVKKNIFLEDEN
                     RWVTVKISTRALRTLRKKGLKAAIKDNGGS"
     gene            448..834
                     /locus_tag="PK683_18260"
     CDS             448..834
                     /locus_tag="PK683_18260"
                     /inference="COORDINATES: protein motif:HMM:NF019415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HNH10451.1"
                     /translation="MENMNTLEAYENLKKVNFSDEQSREIVKMFVKYYEAEPASKKDL
                     RDTESNLNQDLKNTELRLQKEIEGIKKETQDIRLEIKNVELKLSKEIEGVKLEIQHIK
                     VDFIRWLIGVAIASLGLILTGIRFLR"
     gene            954..>1330
                     /locus_tag="PK683_18265"
     CDS             954..>1330
                     /locus_tag="PK683_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10452.1"
                     /translation="MESAVEISVRRLGEAYEKAITMSIEEEKFRDFDPLWIDIWLDKW
                     NRMPEKIQNASQLGQIHIQVYWDQHFRLSSFPKKMFPSLADLLCFLRYSADQNNEWLY
                     RRIFPHNMSLSETLSFHQELWAV"
BASE COUNT          392 a          247 c          285 g          406 t
ORIGIN      
        1 gagatccacc attgtctttg atagcggctt tgagaccttt tttacgaaga gttctcaggg
       61 ctcttgtaga gatttttact gtgacccaac ggttttcatc ttcgagaaaa atgttctttt
      121 ttacgatatt gactttccag attcttctgg ttttcaggtg ggagtgagaa accgcatttc
      181 catacattgt tcctttccct gtaactacac atttacgagc catataagta ccttttgcac
      241 cagttttttt ctgtccattg tactgtcaat gaaaaccttt gtgtacatta aaaatcgaaa
      301 ccaaccggtc tcggccgatt tttagaattc cgacaccctg ctgatttcgc tggagcaaac
      361 attcacttcg aggcaaaaat tttccaaagg atgcaaactt gtacagaaaa attcaattct
      421 tgacacaatc cgaaagatac ttgtactatg gaaaatatga atactcttga ggcatatgag
      481 aatctgaaga aggtgaattt ttcggatgag cagtcccgcg aaatcgtgaa gatgttcgtg
      541 aaatactatg aggctgagcc ggctagcaaa aaggatctga gagatacaga gtcaaatttg
      601 aatcaggatc tgaagaatac agaacttcgg cttcagaaag agatcgaagg aatcaagaaa
      661 gagacgcagg acattcggct cgaaattaag aatgttgaac tgaaactttc gaaagaaatt
      721 gaaggagtga aactggaaat tcagcatatc aaggtcgatt ttatccgttg gctgatcggt
      781 gtggccattg cttctctcgg tctgattctt actggaatcc gcttcttaag ataatatctt
      841 ttattcctat actgccgatt tttgaattcc agaaacaact gtcaaaatta aagtcgaaat
      901 tcttaagcac tctttatact gtgcattggg atttgtaaaa gggagacaga tagatggaat
      961 ctgcagttga aatttctgta agaagacttg gtgaggctta cgagaaagca attacgatga
     1021 gcattgagga agaaaagttt cgtgatttcg atcctctctg gatagatatc tggctggata
     1081 aatggaacag gatgccggag aagattcaaa acgcttctca actcggacag attcatattc
     1141 aagtttactg ggatcagcat ttccggttgt cttctttccc caaaaaaatg tttcctagtc
     1201 tagcggatct gctctgtttt ttgcgttatt cggcggatca gaacaatgaa tggctgtatc
     1261 ggcgaatttt ccctcataat atgagccttt cggaaactct gagttttcat caggaactgt
     1321 gggctgtctt
//
LOCUS       DAOQGJ010001408         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80372_length_1330_cov_2.692549, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001408 DAOQGJ010000000
VERSION     DAOQGJ010001408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80372_length_1330_cov_2.692549"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1330)
                     /locus_tag="PK683_18270"
     CDS             complement(<1..>1330)
                     /locus_tag="PK683_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10453.1"
                     /translation="KAGDSEFFGVINIGDVPTFLNLCEGKSLTVEKEEFGSSLFHSIN
                     RRDSQINILIGSKKFTEGWNSWRVSCMGLLNIGKNEGAQIIQLFGRGVRLRGYSHSLK
                     RSRFLNHLPDSVEVPDNMILAETLNIFGIHADYIQKFQEYIEAEGVIEESKKTQIEIP
                     VKKLQGIEDLKILDLKKGFSFSKTETLMLTGPESKYLEALQKRPIVLNWYTKIQGFSS
                     KYADTAGKSVEYECHFFQDWHIPFLNLDRLYFELVDFKKSRGFYNLLIQKKTIKELLL
                     NKNWYLLEIPEKEFSLSAENKEITWQEIALSLLQKYISRFYHFQKDAFESDKKEYIKI
                     GEYEKRKNKSGTVGNLFSNYNVSLNPQDKELIEYFTELNAELKKTSYNSAKEHTPASP
                     KIVPLLLDHHLYKPVLFHDTDVLVHISPSAITASSEKIFLEAFELFAKEKS"
BASE COUNT          387 a          261 c          227 g          455 t
ORIGIN      
        1 ctgatttctc ctttgcaaac aactcaaatg cttccagaaa tattttttca ctggatgctg
       61 taattgcaga tggagaaata tgaaccaaaa catctgtatc atgaaaaaga accggtttat
      121 aaagatggtg atcgagcaaa agaggaacaa ttttcggtga agcaggagtg tgctcttttg
      181 ctgaattgta tgaagtcttt ttaagttcag cattgagttc agtaaaatac tctatcagtt
      241 ccttgtcttg aggatttaga gatacattgt aattactgaa taaatttccg acagtacctg
      301 atttgttttt tcttttttca tattctccga ttttaatgta ttcctttttg tctgattcaa
      361 aagcatcttt ttggaaatga taaaagcgtg atatgtactt ctgcagaagc gaaagtgcaa
      421 tctcctgcca agtgatttcc ttgttttcgg cagaaagaga aaattctttt tccggaatct
      481 ccaacagata ccagttttta ttcaaaagca gttccttgat tgttttcttc tgaatgagca
      541 gattataaaa tcctctggac tttttaaaat cgactaattc aaagtagagc ctgtccagat
      601 ttaaaaacgg gatatgccaa tcctgaaaaa aatggcattc atactctact gattttccgg
      661 cagtgtcagc atacttgctg gaaaaacctt gaatttttgt gtaccaattt aatacaatag
      721 gtcttttctg caaagcttca agatacttac tctccggacc tgtcagcatc agtgtctctg
      781 tttttgaaaa actgaatccc tttttcaagt ccaaaatttt caagtcttca atgccttgca
      841 atttttttac gggaatttca atctgagttt tttttgattc ttctatgacc ccttccgctt
      901 caatgtattc ctgaaatttc tggatataat ctgcatgtat tccaaaaata ttcaaagttt
      961 ctgcaagaat catattatct ggtacttcaa cggaatcagg cagatgattt aaaaaccggc
     1021 ttctcttcag gctgtgactg tagccgcgaa gccttacccc tctcccaaaa agctgaatga
     1081 tctgagctcc ttcatttttt ccgatattca acagtcccat acaggaaaca cgccagctgt
     1141 tccagccttc tgtgaatttt ttggatccga tcagaatatt gatctgagag tcccgtctgt
     1201 ttatggagtg aaacagagaa gagccgaatt cttctttctc gactgtcaga gattttcctt
     1261 cacaaagatt taagaaagtc ggaacatctc caatattgat tactccaaaa aattctgagt
     1321 cacctgcttt
//
LOCUS       DAOQGJ010001409         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80384_length_1330_cov_2.453333, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001409 DAOQGJ010000000
VERSION     DAOQGJ010001409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80384_length_1330_cov_2.453333"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PK683_18275"
     CDS             complement(<1..216)
                     /locus_tag="PK683_18275"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10454.1"
                     /translation="MYIKEIKIQNIRSIENFEMKFEDGEEAGWHVLLGDNGTGKTTIL
                     QSIGIGFLEQVYLINPNFQFKSSSDGST"
     gene            complement(401..>1330)
                     /locus_tag="PK683_18280"
     CDS             complement(401..>1330)
                     /locus_tag="PK683_18280"
                     /inference="COORDINATES: protein motif:HMM:NF027399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNH10455.1"
                     /translation="SEDYQARASVSVVMIFVTQVAHYHLFTLKGYDPAKTAPHTAGAT
                     GHSQGIISASFAALALSGAEFYKALENYIDYMFFLAYHAQGGFMTFDYSKEVIEGNAA
                     IGDKNPAPMVAVIGYSKEELEERVAKANSELGLHGQDQLFISLYNTPDSMIISAKPES
                     LLKFRNKYKAEMDETKRKFVYLKTSAPFHCPFMENTWAPFKADFDAGKFSFPYKISDL
                     KYPVYSIFDGQDYRTKSVDLLEVLFKDVVIRPLYWDKAVGALLTGSEIGYAVDFGPSV
                     VSSRLTGGQLTPKNITTPVYCAANPKDLKNLFE"
BASE COUNT          353 a          279 c          287 g          411 t
ORIGIN      
        1 agtgcttcca tcactactgc tcttgaattg aaaatttgga tttattaaat aaacttgctc
       61 aagaaatcca atgcctattg attgaagaat cgttgtcttc cctgttccgt tgtcgcccag
      121 aagcacatgc cagcctgctt cttcaccatc ttcaaatttc atttcaaaat tttcaataga
      181 acgtatattc tgaatcttta tttctttgat atacataatt tattcccttt cttattcaat
      241 atcgggcttg tatttgaagt gacaatcata attcattgtt gttcggtgta tgttctttat
      301 ccaagtccta ttctgcctgg tattgagtga atcctgatcg aatcaaatat ttatagaagc
      361 gaactctatg ccgcaaaggg ttcccttgcg gcagattttt ttactcgaaa agatttttca
      421 ggtcttttgg gtttgctgca cagtagactg gcgtagtgat gtttttaggt gtgagctgtc
      481 cgcctgtaag tcttgaggaa actacacttg gtccaaagtc gacagcataa ccgatttcag
      541 aaccggtcag aagtgctccg actgccttgt cccagtacaa tgggcggatg actacatcct
      601 tgaagagaac ttcgaggaga tccacagatt ttgttctata gtcctgtccg tcgaatatgg
      661 agtatacagg gtatttcaaa tcggatattt tataaggaaa agaaaatttt ccggcatcaa
      721 agtccgcctt gaaaggagcc caggtgtttt ccatgaaagg acagtgaaac ggagcagaag
      781 tcttgagata tacaaacttt cttttggtct catccatctc tgccttgtat ttgtttctga
      841 atttcagaag ggattccggc tttgcagaaa tgatcatgga atcaggagta ttgtacagac
      901 tgataaaaag ctggtcctga ccatgaagac cgagttcaga atttgccttg gcgactcttt
      961 cttcgagttc ctctttggaa tatccgatca ctgccaccat cggagcaggg ttcttgtctc
     1021 cgatcgcagc atttccttca atgacttcct tggaataatc gaacgtcata aatccgccct
     1081 gagcatgata tgccaggaaa aacatatagt cgatatagtt ttcaagagcc ttgtagaatt
     1141 cggcaccgga aagtgcaagg gcagcgaatg aggcagagat gattccctgg ctgtggcctg
     1201 ttgcgcctgc agtgtgagga gccgtttttg caggatcata tcctttcaga gtgaacagat
     1261 gataatgagc cacctgagtg acaaagatca tcactacaga cacgctggct ctcgcctggt
     1321 agtcttcaga
//
LOCUS       DAOQGJ010001410         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80404_length_1330_cov_2.202353, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001410 DAOQGJ010000000
VERSION     DAOQGJ010001410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80404_length_1330_cov_2.202353"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..>1330)
                     /locus_tag="PK683_18285"
     CDS             complement(125..>1330)
                     /locus_tag="PK683_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH10456.1"
                     /translation="RYGVQVNSLGLTEEQPENNAWRIILEALGVTLSRKARCRALQLP
                     AWNEALSLPRPWDQQWSLRLQQILAYESDLLEYPDIFDGSKVIESKVSELKKDAYAEI
                     HKILDMGGAIKAIENGYLKSQLVKSQSERMSKINSGEQIVVGKNKWTEAIQSPLMTDT
                     DGGIFKVDPESAAQTLKSLEETKRKRDPEKVKSALEKLKAAAKNNENMFPASIECAKA
                     GVTTGEWSDILRETFGTYQPSTGVEGQVLKIENDTVKQVREKVDKFFQSKGHRPRIVV
                     GKPGLDGHSNGAEMIAISARHAGFDVIYSGIRLTPEEIVQSAVEENADVIGISILSGS
                     HKEIAEQISSELVHYKAKEQIPVVFGGIIPESDFGSLKSLGVKEIFTPKDYDLMKIMD
                     KIIDIISVK"
BASE COUNT          317 a          323 c          271 g          419 t
ORIGIN      
        1 atgcagatcg aatgaatccc tcgtcgtctg ttttatattc cagaaactgc agtgacggaa
       61 tctttttata gaattccgtc atcgcagtca gattctttgt gccaatcgca atatgatgca
      121 cagattactt cactgaaata atatctatga tcttatccat aattttcatc aggtcatagt
      181 ccttaggagt gaatatctcc tttactccga gtgacttcaa tgatccaaaa tcagactcag
      241 ggatgattcc tccaaataca accggaatct gctcctttgc cttgtaatga acaagttctg
      301 aagaaatctg ctctgcaatt tctttatgag aaccggaaag aatggagatt ccgatgacat
      361 ctgcattttc ttccacagca ctttgaacga tttcttcagg agtcagtctg attcctgaat
      421 agatcacatc aaaacctgca tggcgcgccg aaatggcaat catctctgct ccgttggaat
      481 gcccgtcaag tcccggctta ccgactacaa ttctaggtct gtgtcctttg ctctggaaaa
      541 atttatctac cttctccctt acctgcttta cagtatcatt ttcgattttc agaacctgtc
      601 cttccactcc ggttgagggc tgataggtcc cgaatgtttc acgaagaata tcggaccatt
      661 ctcctgtagt gactcccgcc ttcgcgcatt caatggatgc agggaacatg ttttcattat
      721 tttttgctgc ggccttaagc ttttccagtg cagatttgac tttctcagga tctctttttc
      781 tttttgtttc ttccagagat ttcagtgtct gcgctgccga ttcagggtcc accttgaata
      841 tacccccatc ggtatcagtc atcagaggag actggattgc ttcagtccat ttgttctttc
      901 cgactacaat ctgctcgccg gaattgatct ttgacattct ttcggactgt gattttacga
      961 gctgagattt caagtatccg ttttcgattg ccttgattgc tccgcccata tccaatattt
     1021 tatgaatctc tgcgtaagcg tccttcttca gttcagatac cttgctttca atgacttttg
     1081 atccgtcaaa aatatccgga tattccagaa gatcagattc ataggcaagg atctgctgga
     1141 gccggagtga ccactgctga tcccaggggc gggggaggga gagtgcttca ttccacgcag
     1201 gaagctgaag tgcacggcat ctggcttttc tggaaagagt gaccccaagt gcttcgagaa
     1261 tgattctcca ggcgttgttc tcaggctgct cttcagtaag accgagcgaa tttacctgaa
     1321 ccccgtaacg
//
LOCUS       DAOQGJ010001411         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80452_length_1329_cov_3.689953, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001411 DAOQGJ010000000
VERSION     DAOQGJ010001411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80452_length_1329_cov_3.689953"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1329
                     /locus_tag="PK683_18290"
     CDS             <1..>1329
                     /locus_tag="PK683_18290"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH10457.1"
                     /translation="TGVIALASYLFKRDFSLTIEAGELQITHILSTQMESDLLSLSQN
                     LKLFLKDAKLGKCDENFFENDNNILFAGVFGKKDPGIEMMTGCTNPRILNKDFQSSAD
                     FAKAAQNHIAETALSFDGKTVLRNASLSFKSPVLMFAFPFQTKDNQSEIVIMFAKHSL
                     LGQSFQGNGMSETFLVDENGNTIYHEDSKNVETAKNLNEIPIVASVAESKVDTAQHIF
                     NYKNEDFIGTFNKLNFTGGSVISIIQVKKILEPVNAMQRNIIFLMLAVLSISLLIVFL
                     FSRSITIPVKELVIGTQRIQTGDYSTYIIPSSGDEIGSLTNSFNSMSKGLDEREKMKK
                     AFSKFVNEEIADLAAQGEIKLGGERKFCAIFFSDIRGFTSISEKLEPEEVVEFLNQYM
                     TEMVTCVKKTGGIVDKFIGDAIMATWGALKHLPNETECAVNASLMMRTALL"
BASE COUNT          410 a          277 c          292 g          350 t
ORIGIN      
        1 actggcgtca ttgcactggc ctcctatctg ttcaagcgcg acttcagtct gacgattgaa
       61 gcgggtgaac tgcagatcac tcatattctt tccactcaga tggaatcaga tcttctttct
      121 ctttctcaga atctgaaact gttcctgaaa gatgccaaac tcggcaaatg tgacgaaaac
      181 ttttttgaaa acgacaataa cattctgttt gcaggcgtat tcggcaaaaa agatcccggc
      241 atcgaaatga tgacaggatg cacaaaccca agaattctga acaaggattt tcagagctct
      301 gccgacttcg caaaagccgc acagaatcat attgcagaaa ctgcattgag ttttgacggc
      361 aagacagttc tgagaaacgc cagtctgagt tttaaatctc ctgtgcttat gtttgctttt
      421 ccttttcaga caaaggacaa tcagtcggaa atagtcatta tgtttgcaaa gcattctctt
      481 ctgggccagt catttcaggg gaacgggatg agtgaaacat ttcttgtgga cgagaacgga
      541 aacacgatct atcatgaaga ctccaagaat gtggaaaccg cgaaaaatct gaatgagata
      601 cctatcgtgg cctcagttgc agaaagcaag gtggatactg ctcagcacat cttcaattac
      661 aagaatgaag acttcatagg aacattcaac aagctgaatt tcaccggcgg ttcagtcatt
      721 tccataattc aggtcaagaa aattcttgaa cctgtgaatg caatgcagag aaatattata
      781 ttcctgatgc tcgctgtatt gagcatttca cttctcatag tatttctttt ctccagatca
      841 attacaattc cagtgaagga acttgtcatt ggaacgcaga ggattcagac tggagactac
      901 agcacgtata tcattcccag ttcgggggat gaaatcggat cgctgacaaa ctccttcaac
      961 tcaatgagca agggactcga tgaaagagaa aagatgaaga aggctttcag caagtttgtg
     1021 aatgaggaaa ttgccgatct tgctgctcag ggagaaatca agcttggcgg ggaaagaaag
     1081 ttctgcgcaa tattcttctc cgatatcaga ggattcactt cgatctctga aaaacttgaa
     1141 cctgaagaag tcgtggagtt tctcaatcag tacatgactg aaatggtgac ctgcgttaaa
     1201 aaaaccgggg gaatagtaga taaattcatc ggtgatgcca tcatggcaac ctggggtgca
     1261 ttgaaacatc ttcccaatga aacagagtgc gcagtaaacg catcacttat gatgagaact
     1321 gctcttctc
//
LOCUS       DAOQGJ010001412         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80540_length_1328_cov_5.213668, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001412 DAOQGJ010000000
VERSION     DAOQGJ010001412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80540_length_1328_cov_5.213668"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PK683_18295"
     CDS             <1..689
                     /locus_tag="PK683_18295"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNH10458.1"
                     /translation="MILIIDPFLKQPAAEAFNVISYLHHQAQKELNSFQSYIEIFFPL
                     YSNISLEEYISRLHQNGRKIIGVISLGSYVNITDRPEWINLFGKDLKENIIEQSIPFL
                     GICFSHQLFGWIYGSNVDYIQNRDQIPDGKYNEFRNIKVSHDRLKNILGGKSSFISKA
                     KHEQEVKTVNDEFLEICCTGEKCRIEGLMHRKFPSFSVQSHPEEFHESGDGFHFIKNF
                     IYFFINSAHK"
     gene            686..>1328
                     /locus_tag="PK683_18300"
     CDS             686..>1328
                     /locus_tag="PK683_18300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH10459.1"
                     /translation="MNILINLMAAVLLFGFAYIIWNYAKGLKDSPREIWILFGAKLIE
                     YAAYGASNMTFVLYLSEDCGLGDVEAGSYIGTWSTLLTCFSILVGSVVDAVGIRKTLV
                     IGMTFLLIGRFVMPFTSNLWTATFFAFIPVAAGNALLGPVLSVGIKKFTTKTSSTLGF
                     GLFYTLMNTGFALGGYIFDKTRSVIGEHSSVKIPLFQINMSTYQIIFAVSFLLS"
BASE COUNT          413 a          241 c          266 g          408 t
ORIGIN      
        1 aaatgattct gattattgat ccgtttttga aacagccggc agcggaggca ttcaatgtta
       61 tttcttatct gcatcatcag gcgcagaaag aactgaattc ttttcaatct tatattgaaa
      121 tctttttccc gctgtactcg aatatttcat tggaagaata tatcagcaga ttacatcaga
      181 acggaagaaa aattattgga gtgatttcac tcggaagcta tgtaaatatc actgacagac
      241 ctgaatggat aaacctattt ggaaaagatc tgaaggaaaa tatcattgaa cagagtattc
      301 cattcctggg tatatgtttc tctcatcagc ttttcggatg gatttacgga tcaaatgtgg
      361 actatataca gaaccgagac caaataccgg acggaaaata caatgaattc agaaatatta
      421 aagtaagtca tgacagattg aaaaacattc tgggaggaaa atccagtttc atctcaaaag
      481 caaaacatga gcaggaagtg aagacagtca atgatgaatt tctggaaatc tgctgcacag
      541 gagaaaaatg cagaatagaa ggtcttatgc acagaaaatt cccttcattt tcagtccagt
      601 ctcatcctga ggaatttcac gaatcaggag atggattcca ttttatcaaa aattttatat
      661 atttttttat taattcggct cacaaatgaa tattctgata aatcttatgg ctgctgtcct
      721 tctttttgga tttgcctata ttatatggaa ctatgcaaag ggactgaaag attctccaag
      781 ggaaatctgg attcttttcg gagcaaaact cattgagtat gctgcatacg gtgcatccaa
      841 tatgacgttt gtgctttatc tatcagaaga ctgcggactg ggagatgtgg aagcaggctc
      901 atacatagga acctggtcga cgcttttaac ctgtttttcc attctagtcg gttccgtagt
      961 ggatgccgtg ggaatcagga aaactcttgt cataggaatg accttccttc ttatcggcag
     1021 atttgtgatg ccgtttacat caaatctgtg gacagcaacc ttctttgcct ttatcccagt
     1081 cgctgcggga aatgctcttc taggaccggt actttctgtg gggatcaaaa agtttacaac
     1141 gaagacaagc tccactctcg gattcggtct tttttataca ttaatgaata cagggtttgc
     1201 actgggagga tatatatttg acaaaacaag atctgtaatc ggagaacaca gctcagtaaa
     1261 gattccatta tttcaaatca atatgtccac atatcagatt atatttgcag tgagctttct
     1321 gctttcta
//
LOCUS       DAOQGJ010001413         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80556_length_1328_cov_3.656716, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001413 DAOQGJ010000000
VERSION     DAOQGJ010001413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80556_length_1328_cov_3.656716"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK683_18305"
     CDS             <1..233
                     /locus_tag="PK683_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10460.1"
                     /translation="HVSIRHHYFWSMESEAPVECTVFLSIRKKSGNIAGSASTPDARI
                     REMMLSGYYYSQVEGRYTADPSDDWFEKKIKL"
     gene            complement(262..>1328)
                     /locus_tag="PK683_18310"
     CDS             complement(262..>1328)
                     /locus_tag="PK683_18310"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH10461.1"
                     /translation="LQSVFISFDGSMLVSVFMGVLEEDTGQFFHINAEHPEAVLFRDK
                     KAAFISEEKPEYKIGVELQGLSLTIRETLLRPGDIVIAGSDGRDDILVPEEKTDASSS
                     ESKTMNFNEKIFIQTVERTEGDLEKIIREIQSLGEITDDLSLLRMEYFGGGSRTVVNL
                     DRLSKFLQKEFSVIFLYIHFDESAGSSASSQGIHLYIKEIENELEMVFLSEEFEKLSS
                     LDLAFISKKEMKNDLNHSEQAVRAAVKSLQYIESFNAKHSVKFSLRIGIHSVKSLELE
                     NSKAFLEKEILTAKQIETAGEFGKIRVSESVHDLMQASCKFQSPKKLILSETESIKTY
                     NIYGVSRKLLLDEDRNPDLP"
BASE COUNT          373 a          319 c          235 g          401 t
ORIGIN      
        1 aacatgtatc catccgtcat cattatttct ggtcaatgga atcagaagcc cccgttgaat
       61 gcacagtatt cttaagcatc agaaagaagt ccggaaatat tgctggaagc gcctccacgc
      121 ctgatgcaag aatcagagag atgatgctca gcggctacta ctattcacag gtggaaggga
      181 gatacacagc agatccttcc gacgactggt ttgaaaaaaa gatcaaactc tgatcactgc
      241 attccgagca ccgatccgga cttacggcaa gtccggattt ctgtcttcat ccaaaagcag
      301 ctttctggat actccgtaaa tattatatgt tttaatactt tctgtttcgg acagaatcag
      361 tttcttggga ctctgaaact tgcaggatgc ctgcatcaga tcatgcacac tctcactcac
      421 gcgtatcttt ccgaattcac ctgcagtttc aatctgcttt gcagtgagga tttccttttc
      481 cagaaaagct ttactgttct ccaattcgag tgattttacg gaatgaatcc cgatccgcag
      541 actgaatttt acactgtgct ttgcattgaa actctcaata tactgcagtg attttacggc
      601 tgccctgact gcctgctctg aatgattcag atcatttttc atttcctttt ttgaaatgaa
      661 agctagatcc agagaagaca gtttctcgaa ttcttcactc aaaaacacca tttccagttc
      721 attctcaatt tctttgatat acagatgaat tccctgcgat gatgcagatg aaccggcaga
      781 ttcatcaaaa tgtatataca gaaaaatcac ggaaaattcc ttctgcagaa atttggaaag
      841 tctgtccaaa ttgaccaccg tcctgctgcc tcctccaaaa tattccatcc gaagaagtga
      901 aagatcatcc gtgatctcac ctaatgactg aatttccctt attatttttt ccagatcccc
      961 ttctgtcctt tctacagtct gaataaatat tttttcatta aaattcattg tttttgattc
     1021 acttgaagaa gcatctgtct tttcttccgg aaccagaata tcgtctcgtc cgtcagaccc
     1081 tgcaatgaca atgtctcccg gcctgagaag agtctcccgt atagtcagag aaagtccctg
     1141 cagttcaacc cctatcttat attccggctt ttcttcagat ataaacgctg cctttttgtc
     1201 cctgaaaaga acggcctcag ggtgttccgc attgatatga aaaaactgtc ctgtatcctc
     1261 ttcaaggact cccataaata cggatacaag catacttccg tcaaaggaaa taaagactga
     1321 ctgaagtt
//
LOCUS       DAOQGJ010001414         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80706_length_1327_cov_2.816824, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001414 DAOQGJ010000000
VERSION     DAOQGJ010001414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80706_length_1327_cov_2.816824"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(868..1137)
                     /locus_tag="PK683_18315"
     CDS             complement(868..1137)
                     /locus_tag="PK683_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10462.1"
                     /translation="MTEEAQRHTQAIIIIRKEKKHPGSPIRRRNRKEMKYSSLATLFR
                     KFMIPRIRVISRCRPRKTIEASQEPSVIIWFILWKNLHRKAVTII"
BASE COUNT          382 a          245 c          244 g          456 t
ORIGIN      
        1 taaaaaaatg ataatcagta ttttcagaag taagttcata ttttaatcag tgtatcagct
       61 ttttttcagt gaatcagacc gttccgtctc ttctgattca cttccgtata taaaaggcat
      121 aaaaatgtaa atacgcagat gcaatactcc cggaaaccgg tattaatccg agtacatttt
      181 tttctgtctg tcttaaaatg atttttcgga atgagacaga aaatcaataa tttctttatt
      241 ctctgactgc gtaaaatatg actgaaaaaa tttatgaata actcatttta taaagcaact
      301 gagtgcttgc aggtaaatat tctttaaaat ttcggcttta tgtcaagtat gaaataagtt
      361 gacttatttt tcagggcagg gatctggaag aagcgttttt attccgtaac tcaccttacg
      421 cctacggccg caggtggggc gttttggaca ggaacgcctg tcggctgcac agatgcagaa
      481 attctatttt gagcgtctgc tttaaaaaag caggaaaggg gctctcggaa aaaatggcga
      541 agtgatttat tcttcaaaca gtgcggcagt tcagaaaacc gttgagtttg tccctggtat
      601 ttttattttt caaatgagaa agaactgaag tttccaaatc gcacccgacg ctgcatcaat
      661 ggctgaaact gattggcttg acttacggat gaaaaactct ttcctcttat aatagcaatg
      721 atttcttttt cttgattgaa tgccaatcga ttcgaaaaat tttcagatga gcagagattt
      781 ttggacaaga tttactgcgg aataagaagt atccaatgga gaatgataaa tgaacagaat
      841 acatatcaat ataggaattt actttcttta tattatagta actgcttttc tgtgtaaatt
      901 cttccacaag ataaaccata ttattacaga tggttcttgg cttgcttcaa ttgtcttcct
      961 tggtctgcat ctgctgatta ctcttattct cggtatcata aacttccgaa atagagttgc
     1021 aagggaagaa tatttcattt cttttctgtt tctccttctt attggacttc ctggatgttt
     1081 cttttctttc cttattatta taatagcctg agtatgcctt tgggcttctt ctgtcaaatg
     1141 gccgcccaaa tgggacttct atctgtattc tgcttgaaat tatttttatg ataattcata
     1201 ttataattac acttattttg agtcacccgt acttcttcag attcagccgc agcgcgttca
     1261 gcacaactgt cactgagctg agtgccattg ccgcccctgc aatccatggt gcaaggaaac
     1321 ctgctgc
//
LOCUS       DAOQGJ010001415         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80725_length_1327_cov_2.399371, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001415 DAOQGJ010000000
VERSION     DAOQGJ010001415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80725_length_1327_cov_2.399371"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PK683_18320"
     CDS             <1..1137
                     /locus_tag="PK683_18320"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH10463.1"
                     /translation="VCGAAPMPVEVFHKFEEKFKGKIIEGYGLSEGTCVSSLNPLLGT
                     RKIGSIGISIPGQEMAIWDNEGKELKAGEIGEIVVRGPNVMKEYFRKPEETAKSIEKG
                     WLHTGDLGYRDEEGYYFIVGRKKEMIISGGENIYPKEIEEVLYKHDDISECAVVGLPD
                     KTYGEVVGAFIVPKAGATISEKDVKSFLRPKIAGYKFPKVIEIVTDLPKTATGKIQKN
                     KIVEDHAGNAHLVGKIEGKVNIPYRWVYGSALARFYGGLKNEGKFYGVKCPKCSKVQC
                     PPKAFCGVCFEECTEWLELPNSGILESYTTVHMEFPGQPTKPPYTFGYIKLDGANTHV
                     YHIVSEIPESELKTGLRMEAVWRDKKDRTGTLHDILYFRPVKNV"
     gene            1130..>1327
                     /locus_tag="PK683_18325"
     CDS             1130..>1327
                     /locus_tag="PK683_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNH10464.1"
                     /translation="MYKKGKTFEEIQIGEKASFTKTITETDVYMFAGISGDFNPLHVD
                     EEYAKTTQFRTRIAHGGLAASL"
BASE COUNT          427 a          263 c          338 g          299 t
ORIGIN      
        1 gtctgtggag cagctcctat gcctgtggaa gtctttcaca aatttgagga aaaattcaag
       61 ggaaagatca ttgaaggtta cggactttca gaaggaacct gtgtatcctc actgaatcct
      121 cttcttggca caagaaaaat cggctctatc ggaatctcca ttccgggtca ggaaatggcc
      181 atctgggaca atgaaggaaa agagctgaag gcaggagaga tcggagagat cgttgtccgc
      241 ggtccaaacg taatgaagga atatttccgg aaacctgaag agactgctaa gtcgattgag
      301 aaaggctggc ttcatactgg ggatctggga tacagggatg aagaaggata ctacttcatc
      361 gttggcagaa aaaaagagat gatcatcagc ggcggcgaga acatctatcc gaaggaaata
      421 gaagaagtat tatacaagca tgatgatata tccgaatgtg cagtggtcgg ccttcctgac
      481 aaaacatacg gtgaagtcgt gggtgcattc attgttccca aggcaggggc cacaatctcg
      541 gaaaaagatg tgaagtcctt tctcagaccg aagatcgcag gctataaatt tccgaaggtg
      601 attgagatcg tcactgatct tcctaagacc gccacaggaa aaattcagaa aaacaagatc
      661 gtggaggatc atgcaggaaa tgctcatctg gtaggaaaga ttgaaggaaa ggtcaatatt
      721 ccttacagat gggtgtacgg atctgcactt gcaagattct acggaggact gaagaatgaa
      781 ggaaagttct acggagtaaa atgtcccaag tgcagcaagg tgcagtgtcc ccccaaggca
      841 ttctgcggag tctgttttga agaatgcact gaatggctgg aacttcccaa ttcaggaata
      901 ctggaaagct acactactgt gcatatggaa tttccaggtc agcctacaaa gcccccctac
      961 actttcgggt acattaaact ggacggagca aacactcatg tctatcatat tgtatccgag
     1021 attcctgaat ctgaacttaa gactggactc aggatggaag ctgtatggag agacaaaaaa
     1081 gacagaacag gaactctcca cgacatactc tatttcaggc cggtaaaaaa tgtataagaa
     1141 agggaaaaca ttcgaagaaa ttcagatcgg agagaaggca agcttcacta agacaatcac
     1201 agagaccgac gtctatatgt ttgcggggat cagcggagac ttcaatcctc ttcatgtgga
     1261 tgaggaatat gcaaagacta cacagttcag gacaaggatt gctcatggag gacttgctgc
     1321 atccctt
//
LOCUS       DAOQGJ010001416         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80733_length_1327_cov_2.236635, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001416 DAOQGJ010000000
VERSION     DAOQGJ010001416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80733_length_1327_cov_2.236635"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PK683_18330"
     CDS             <1..680
                     /locus_tag="PK683_18330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH10465.1"
                     /translation="LDIRLEHTVKKISYNDDGVTVETDQGTFKGDYALVTLPLGVLKK
                     GTVKFDPPLPKRKQDAVNRLKMGVMNRLYLKFPKAFWPKEPELMNMYAKKREDYLDMM
                     NFYHYLDKPILLFFTAGNDAVEKESLSDEELIQSVMKSLKKIYGEDIPEPEDYIRKSW
                     SKDEFSYGSYAYIPPGAFPEDHDALGESVDDVLFFAGEATTSDYSGTVHGALISGREA
                     AEEILSD"
     gene            complement(789..>1327)
                     /locus_tag="PK683_18335"
     CDS             complement(789..>1327)
                     /locus_tag="PK683_18335"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HNH10466.1"
                     /translation="MDQEEIVLGLDGKLFTEGSWGHFHEIGHNMQRGEWTFEGTTEVT
                     TNIFTLYNMEKMIKIAPIDNQILKEHRIKYKSYLKKGGDFEEWKKDPFIALMTYLKLQ
                     EKFGWNAYKSVFAQYSSLPDAQKPKNNQDKIDTWVRIFSKTVKMNLFPYFRKWAWPVS
                     DKVNSELKTLPEWKFEDY"
BASE COUNT          384 a          264 c          276 g          403 t
ORIGIN      
        1 gactggatat ccgtcttgag catacagtta aaaaaatatc ctataatgat gatggagtga
       61 ctgttgaaac agatcaggga acattcaaag gagattatgc tcttgtgact cttcctctgg
      121 gagttctgaa aaaaggaaca gtcaaatttg atccgcctct gccgaaaaga aagcaggacg
      181 cagtcaatcg tctcaaaatg ggtgtaatga acagactgta tctcaaattt ccgaaggctt
      241 tctggccgaa ggaacctgag ctgatgaata tgtatgcgaa gaaaagagaa gactatctgg
      301 atatgatgaa tttttatcat tacctggata aaccgatcct gctctttttt acggcaggaa
      361 atgatgcagt ggaaaaagaa tctctttctg atgaggaact gattcagagt gtaatgaaaa
      421 gtctgaaaaa aatatacggg gaggatattc cagagccgga agattatata cggaaaagct
      481 ggtcgaagga tgagttttca tacggatcgt atgcttatat tccgcccgga gcttttcctg
      541 aagatcacga tgcgcttggt gagtctgtgg atgacgttct gttttttgcc ggagaggcga
      601 caacaagcga ctattccggg acagtacacg gagcattgat ttcagggaga gaagctgccg
      661 aggaaatact ttctgactga aattccggtt ttttaattat aagataatta aaaaataatc
      721 tttccttcag aaaactgcat ttcaggaata ccagtcaggc atacagacct gactggattt
      781 tctcaatctc agtaatcctc aaatttccac tcaggcagtg tcttcagttc cgaattgact
      841 ttatctgaaa ccggccaggc ccattttcga aaatacggaa aaagattcat cttcactgtc
      901 ttggaaaaaa ttctgaccca agtgtctatt ttatcctgat tgttcttcgg cttctgcgca
      961 tcgggaagac tgctgtactg tgcaaaaaca gacttgtatg cattccatcc gaatttttcc
     1021 tgaagtttta aatatgtcat cagtgcaatg aaaggatcct ttttccattc ttcaaagtcg
     1081 cctccctttt tcagataact tttatattta atcctgtgct ccttgagaat ctggttgtcg
     1141 atcggtgcaa tttttatcat tttttccata ttatataatg tgaaaatatt cgtagtcacc
     1201 tctgttgttc cctcaaatgt ccattctcct ctctgcatat tgtggccgat ttcatgaaaa
     1261 tgtccccagg agccttctgt gaagagtttt ccgtcaagac cgaggacaat ctcttcctga
     1321 tccatcc
//
LOCUS       DAOQGJ010001417         1326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80861_length_1326_cov_2.014949, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001417 DAOQGJ010000000
VERSION     DAOQGJ010001417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80861_length_1326_cov_2.014949"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..375)
                     /locus_tag="PK683_18340"
     CDS             complement(40..375)
                     /locus_tag="PK683_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10467.1"
                     /translation="MKQFVHCKGKNHLTRTLFFEKTMHFGIIAFSVLFLFCRPKESTA
                     ACESACFAKAALYFGMLQNAGVPHGIENILSTKFQDRCIDHCWDREAGRFPSEFRKRP
                     RHNSDGSRK"
     gene            complement(449..1216)
                     /locus_tag="PK683_18345"
     CDS             complement(449..1216)
                     /locus_tag="PK683_18345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH10468.1"
                     /translation="MLESFKDAILYLGAVSLVFIPLEKAFSVQERPVFRAEFFTDLVF
                     YLGQILVWNELTVSVLRFLFAQERWKFISPFQERVSLLPFWAVLFLTVFFSDLFIYWG
                     HRLQHRISFLWKFHRVHHTALSLDYIAAYREHPLDNIYTRGLETLPAVILGLDLNSIS
                     GFIVFRGLFGLFIHSNTNLRFGFLEKLIGSPHLHHWHHEIEKGGNCNFANLSPLMDIL
                     FGTYYSPEENAENFGIRENIGRGYFEQILESFRFSSK"
BASE COUNT          385 a          273 c          295 g          373 t
ORIGIN      
        1 agaggagaac tctgaaaaat gatgtcctga aaggactgat tattttctgc ttccgtcgct
       61 gttatgtctg ggtctttttc taaactcaga cggaaatctg cctgcttctc tgtcccagca
      121 atgatcaatg cacctatcct gaaattttgt actgagaatg ttttcgattc cgtgcggaac
      181 accggcattc tgaagcattc cgaaataaag ggccgccttc gcaaaacagg cgctttcgca
      241 ggcagcggtg gattctttcg gccgacagaa aagaaaaagg actgaaaaag caatgatgcc
      301 gaaatgcatt gttttttcga agaacagtgt tcttgttaaa tggttttttc ctttgcagtg
      361 aacaaactgt ttcatataag tcaaactcct gttttcagtc tgaggggtgc ttttattata
      421 tattttcttc aataaaaaaa tgatttcatc atttggagct gaatcggaag gattccagaa
      481 tctgttcaaa atatcctctt ccaatattct cgcggatgcc gaaattctcg gcattttcct
      541 caggagaata atatgtaccg aaaagtatgt ccataagggg agagagattg gcgaagttgc
      601 agtttccgcc cttctcaatt tcatgatgcc agtgatgaag atgaggtgat cctatgagct
      661 tttccagaaa gccgaaacgc agattcgtat tggaatgtat gaaaagtccg aataatcctc
      721 tgaacacgat gaaacccgag atcgaattca ggtcgagtcc aaggattact gcgggaagag
      781 tttccagacc cctggtataa atattgtcca agggatgttc cctgtaggct gcgatatagt
      841 ccagactgag agcagtgtga tgcaccctgt gaaatttcca gagaaaagaa atcctgtgct
      901 gcagcctgtg accccagtag atgaaaagat cggaaaaaaa cactgtcaga aaaagaacag
      961 cccagaaagg aagcagtgag accctttcct gaaacggtga aataaatttc caccgttcct
     1021 gtgcaaaaag aaagcggaga actgaaactg tcagttcatt ccagaccagt atctgaccca
     1081 gataaaaaac cagatctgta aagaattccg ctctgaatac aggtctttcc tgcacggaaa
     1141 acgccttttc caagggaata aatacgagcg atactgctcc cagataaaga attgcatcct
     1201 tgaatgactc gagcaatttt atttctctgc ctggggttcc tttgcagggg gattttcttt
     1261 tacggaagga gtttctttgg caggggggtt ctccttttta tcttccttct tttttttgat
     1321 cgagtc
//
LOCUS       DAOQGJ010001418         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80918_length_1325_cov_3.225984, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001418 DAOQGJ010000000
VERSION     DAOQGJ010001418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80918_length_1325_cov_3.225984"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PK683_18350"
     CDS             <1..340
                     /locus_tag="PK683_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNH10469.1"
                     /translation="SVHEYLLLEDIEKYFTPGSTTRVLVMVSDFRGQRGKASIDAEIG
                     SFENRKLKEQILRVTNKSYVLLGVGMGARYIAEELFFNSIQITPDNFSSMPNLLGSAI
                     SNLILVNHRV"
     gene            342..827
                     /gene="smpB"
                     /locus_tag="PK683_18355"
     CDS             342..827
                     /gene="smpB"
                     /locus_tag="PK683_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNH10470.1"
                     /translation="MSKKKEEKAVHTTGEITTNKKAKFNFELLETVEAGIILTGSEVK
                     SLREKKANLTDAFARIRNGEVFLENFHITPYKNGGYANHAEVRSRKLLFKRKEILKLQ
                     KLVREKGLSLVAVKTYFNQRGFVKVLIASAKPKKLHDKRETVQKRESQIEINRAMKGR
                     E"
     gene            824..>1325
                     /locus_tag="PK683_18360"
     CDS             824..>1325
                     /locus_tag="PK683_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002081839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNH10471.1"
                     /translation="MKKYPIVSIVGRQNTGKSTLFNAVFKKNLAITYDYPGVTRDVIK
                     GVYEDSETEQKFLLCDTPGLDIENIDDLSESIIQLAFQQLIDSNVILFLIDKNDIRTY
                     DWKLIKLFREDRRFNDKKILYVLNKADNPDEELDFDQFYRAGIKEIIPISALGRRNLK
                     LLLQTVF"
BASE COUNT          464 a          240 c          299 g          322 t
ORIGIN      
        1 cagtgtacat gaatatcttc ttcttgaaga tattgaaaag tatttcactc ccggttcaac
       61 tacacgagtg cttgtaatgg tgtccgattt tagaggacag agaggaaagg catccattga
      121 tgcggagatc ggctcctttg aaaacaggaa actcaaggaa cagatactca gagtgacaaa
      181 caaatcctat gttctgctcg gggtgggaat gggagcaaga tatattgcag aagaattatt
      241 ttttaattca attcagatca ccccggacaa tttttcgagc atgcccaatc tgcttggaag
      301 cgccatatcc aacctgattc tagtgaatca cagagtctga aatgtcaaag aagaaagaag
      361 aaaaagcagt acatacaacc ggagagataa cgacaaacaa gaaggcaaaa ttcaattttg
      421 agcttctgga aacagtcgag gcaggaatta ttcttaccgg ttccgaagtc aaaagtctca
      481 gagaaaagaa agcgaacctg acggatgcgt ttgccagaat ccgaaacggc gaagtttttc
      541 tggaaaattt tcatatcact ccttacaaaa acggggggta tgcaaaccat gctgaagtca
      601 gatccaggaa actgctcttt aagagaaagg aaatactgaa gcttcagaag ctggtcagag
      661 aaaaaggact gagtcttgtg gcagtgaaga cttattttaa tcagagagga tttgtaaagg
      721 tacttatcgc atctgcaaaa ccgaaaaagc tgcacgacaa gagagagact gtacagaaga
      781 gagagtctca gatagaaata aacagagcca tgaagggcag agaatgaaaa aatatccgat
      841 tgtatccata gtgggcaggc agaacacagg aaagtccacc ttattcaatg cagttttcaa
      901 aaagaatctt gcaatcacat atgattatcc gggagtcaca agagacgtaa tcaaaggagt
      961 gtatgaagac tcggaaaccg agcagaaatt tcttctttgc gacactcccg gtctggacat
     1021 tgaaaatata gatgatctgt cggagagcat catacagctt gcatttcagc agctgattga
     1081 ttccaatgtg attcttttcc tgattgacaa gaatgatata cgcacttatg actggaagct
     1141 gataaagctt ttcagagaag acagacgttt taatgataaa aaaatactgt atgtactgaa
     1201 taaggcagac aatccggatg aagaactgga ttttgatcag ttttaccgag caggaataaa
     1261 ggaaatcatt cctatttccg ctctcggaag aagaaatctg aaactgctcc tgcagacagt
     1321 atttt
//
LOCUS       DAOQGJ010001419         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_80951_length_1325_cov_2.466142, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001419 DAOQGJ010000000
VERSION     DAOQGJ010001419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80951_length_1325_cov_2.466142"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PK683_18365"
     CDS             <1..207
                     /locus_tag="PK683_18365"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNH10472.1"
                     /translation="DEKSRNKAKQIIDLVNSARERLIQLKTEKLSEEERKKAEETFRK
                     EFISSSLPGEYFELKKSVWELKNE"
     gene            257..>1325
                     /locus_tag="PK683_18370"
     CDS             257..>1325
                     /locus_tag="PK683_18370"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNH10473.1"
                     /translation="MNPEKKIVLIDDDVNYINIFAKQVKRFLDSSKTELQTFLNPENA
                     ISYIESNWQSISVIITDFNMPKVNGVELIQKIEKKERIYQIIILTGELNRTITESFEH
                     LNLFDILKKKEPFPKILESLKQAHLFHQNKLDKLNKVESYKKIFSKDSDKTEEKRLSL
                     QDILGDSENMKNVKKKIALVSDNSTACFLFGESGIGKEFIANVIHDISGRSGKFISLN
                     CGTGTGKETEQKLFGMKDGDLNSLLEKADNGTLFLNDIHLLSIDAQNRLLRFFDPGFF
                     AKLSMRRLNIRWISSAPSSIYEQIKQRKFKEDLFYRLNIASIEVPSLRERKDDIRVLT
                     DHFLTQISAAEGKITQKIEEKV"
BASE COUNT          492 a          234 c          254 g          345 t
ORIGIN      
        1 gacgaaaaat ccagaaacaa ggcaaaacag atcatagacc ttgtaaattc tgccagagaa
       61 agactgattc agcttaagac agaaaaactt tcagaagaag agaggaaaaa agcggaagaa
      121 accttcagaa aggagtttat ctcaagcagt cttcctggag aatattttga attaaaaaaa
      181 tctgtctggg aattaaaaaa tgaataaaat tctttacatt ataagcccat gtatgattgt
      241 ctcaatttga gacaatatga atccggaaaa aaagattgtt ctgatagatg atgatgtcaa
      301 ttatattaac atcttcgcca agcaggttaa gagattttta gactcaagca aaactgagct
      361 tcagacattt ttaaatcctg aaaatgcaat ctcttacata gaatccaatt ggcagtccat
      421 ttctgtaatc atcacagatt ttaatatgcc taaagtgaac ggcgtagaac tgatccagaa
      481 aatcgaaaaa aaagaaagga tttaccagat catcatcctt accggcgaac tgaacaggac
      541 aataacagaa tcattcgagc atttgaatct ttttgacata ttgaaaaaaa aggaaccatt
      601 ccctaaaatc ctggaaagcc tgaaacaggc ccatcttttc catcagaata aactggataa
      661 actgaataaa gtggaaagct acaaaaagat tttttccaag gattctgata agacggaaga
      721 aaagagactc tctctacagg acattctggg tgattccgaa aatatgaaga acgtaaaaaa
      781 gaaaattgca cttgtttcag ataattccac agcctgtttt ctgttcggtg aatcaggcat
      841 tggaaaagaa ttcattgcaa acgttattca tgatatttca ggcagatcag gcaaattcat
      901 cagtctgaac tgcggaaccg gtacaggaaa ggaaacagaa cagaagcttt ttggaatgaa
      961 ggacggggat ctgaattctc tgcttgaaaa ggcagacaac ggtacccttt ttcttaatga
     1021 tatccatctc ctaagcatag atgcacagaa cagactgctc cgcttttttg atcctggttt
     1081 ttttgcaaaa ctgagtatgc gcagactgaa tatcagatgg atttcctctg ccccttcttc
     1141 tatttatgaa cagataaagc agagaaaatt caaagaagat ctgttttaca gactgaatat
     1201 tgcatcgatc gaagttccgt cattaaggga aagaaaagat gatatcagag ttctgacaga
     1261 tcatttcctg acccagatat ccgcagccga agggaagatt acacagaaga tcgaagaaaa
     1321 ggtat
//
LOCUS       DAOQGJ010001420         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81085_length_1324_cov_2.018913, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001420 DAOQGJ010000000
VERSION     DAOQGJ010001420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81085_length_1324_cov_2.018913"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PK683_18375"
     CDS             <1..877
                     /locus_tag="PK683_18375"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNH10474.1"
                     /translation="EKNLSSKLRYSAGWKKEFSGLLLKEWGDYYSLSEGKFLPDEEKI
                     EKSRYLNSKFVQMLWKGSGIPMEICRSEDDLKNFALEYGYPFALKEPLSFSGTGNRIF
                     CSEKDLSRFSLNFEKGYLIEKWMERTEDVSGIFHSDEDGIRILDITYMMIDHKGGYNG
                     NLILSGKMPELSSVLLKKTEDLFWKKGIEYNGPFSVDCFSYRDKDGIKFHYFSEFNFR
                     YSFGRIHSDIRRRRRFKGDSLLLFFKPEDISEEIIRKRFRENGKYSRADLILLSPAEI
                     RGQSLRNIPVYIELT"
     gene            complement(885..>1324)
                     /locus_tag="PK683_18380"
     CDS             complement(885..>1324)
                     /locus_tag="PK683_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNH10475.1"
                     /translation="VYYSGLTVGEVVNLKIADFDSDKRLLKIRSQKKERTTYLPESGM
                     NAVEKYLENLGKPMYLFPGRDSIKPVSVRNAEKIFTGALKKAGIPRQVSIHALRHAFA
                     YHRLEEGAELEELQKLLGHKNLQSTKIYAFPERAKREKLKLCV"
BASE COUNT          386 a          243 c          295 g          400 t
ORIGIN      
        1 ggagaagaat ctttcttcaa aactgagata ttcagcagga tggaagaaag aattttccgg
       61 acttcttctg aaggaatggg gcgattatta ttctctgtct gaaggaaaat ttctgccgga
      121 tgaagaaaaa attgaaaaaa gcagatatct caattccaaa tttgtgcaga tgttatggaa
      181 aggttcaggt atcccgatgg aaatctgcag atcggaagat gatctgaaaa actttgccct
      241 cgaatatggt tatccctttg cactgaagga accgctgagc ttttccggta caggaaacag
      301 aatcttctgt tcagagaagg atctgagcag attcagtctg aactttgaga aaggatatct
      361 cattgagaaa tggatggaaa gaacagaaga tgtcagcgga atatttcatt cagatgaaga
      421 cgggattcgg atactggata taacctatat gatgattgat cataagggcg gatacaacgg
      481 caatctgatt ctttcaggga aaatgcctga actttcctcc gtgcttctga aaaagacgga
      541 ggatcttttc tggaaaaagg gaatagaata taacgggcct ttttctgtcg actgtttcag
      601 ttacagagac aaagacggga taaaatttca ttatttttcg gagttcaatt tcagatattc
      661 cttcggaagg attcattcag atatcaggag gaggaggcgt ttcaagggag acagtcttct
      721 tctgtttttc aagcctgagg acataagcga ggaaatcatc aggaaaagat tcagggagaa
      781 tggaaagtat tccagggcgg atctgatcct tctttctccc gcagaaattc ggggacagtc
      841 attaagaaat atccccgttt atattgaatt gacataaatt atattcatac gcagagtttc
      901 agtttctctc tttttgccct ctctggaaat gcatatatct ttgtggactg aagatttttg
      961 tgcccgagca atttctgaag ttcttcaagt tctgcgcctt cttccagcct gtggtaggcg
     1021 aatgcgtgac ggagagcatg aatggatacc tgcctgggaa ttccggcctt cttcagtgcg
     1081 cctgtaaata tcttttctgc atttctgact gaaacaggtt ttatcgagtc tcttccagga
     1141 aaaagataca tcggttttcc cagattttcc agatattttt ccactgcatt catccctgat
     1201 tcaggaagat atgtagtcct ttcttttttc tgagaacgta tcttcagcag tcttttgtca
     1261 gaatcaaaat cggctatctt gagatttaca acttcgccta ctgtaagtcc tgaataatat
     1321 acca
//
LOCUS       DAOQGJ010001421         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81206_length_1323_cov_2.023659, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001421 DAOQGJ010000000
VERSION     DAOQGJ010001421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81206_length_1323_cov_2.023659"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..708
                     /locus_tag="PK683_18385"
     CDS             289..708
                     /locus_tag="PK683_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10476.1"
                     /translation="MNTKFTVKIITICFLLFLAPALFASDPFDGFWLSNPSGNMTNLE
                     TREKAFVLNMYGRNYKFTGSYKVEGGKLHYTAADGKDFTDGTPLKGKKGICKILKLND
                     NLSFQEELKCTGPLEKFRLFGKSLQGAQFGKSTGLKF"
     gene            705..995
                     /locus_tag="PK683_18390"
     CDS             705..995
                     /locus_tag="PK683_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10477.1"
                     /translation="MTIDAGGQTRKKTVLHSAPNASSKSLSCTLWDQNGKKEFEGKGL
                     KSGVYLRVIAKMKENQDRENAWYYVDASAVHENSCGPGWVQEEFLSIAHGGG"
BASE COUNT          386 a          243 c          294 g          400 t
ORIGIN      
        1 gaagaaatct atatttctga acccggcgct gaaaagaagc tgtttttcgt cgtcctttct
       61 gttctccggc tttacattca gaaaaaatga aaagctgtca ccgatatttt tcgtatggat
      121 ctgcagtttt caatcctttt ttcttcccga caattaagaa agatccttgc attgtccgat
      181 ccgagtgctt ttacaaaaag cgatgagttt tacttcgata ctgattccag gaaatgtaaa
      241 attctgcctt tctgttgact gaacaggaaa acctattatg ctggccgaat gaatacaaaa
      301 tttacagtaa aaataattac aatctgtttc cttctgtttc ttgcacctgc actgttcgca
      361 agtgatccgt tcgatgggtt ctggttaagc aatccttcag gtaatatgac aaatcttgaa
      421 accagggaaa aggcattcgt gctgaatatg tatggcagaa attataaatt caccggctcc
      481 tataaagtgg agggaggtaa attacactat actgctgctg acggaaagga ctttactgac
      541 gggacacctc tgaaaggaaa aaaaggtatc tgtaaaattt taaaactcaa tgataatctt
      601 tcctttcagg aagagctgaa atgcactggc cctcttgaaa aattccgttt attcggaaaa
      661 agtctccagg gggcacagtt cggaaagtca acgggattga agttttgaca atagatgccg
      721 gtggacagac aaggaagaag actgttctgc attctgctcc aaatgcatca tccaaatctc
      781 tttcctgtac tctgtgggat caaaacggga aaaaagaatt tgaggggaaa ggtctgaaga
      841 gtggtgtgta tctgcgggtt atcgcaaaga tgaaggagaa tcaggaccgg gaaaacgcct
      901 ggtactatgt agatgccagt gccgttcacg aaaattcctg cggtcccgga tgggtgcagg
      961 aagaattcct gtcgattgct catggaggag gctgattcct taaagtgctc aacatataaa
     1021 gatatataat atctttatat gcagacttgc ttggtgtcct caggaatgcg gaaatcctat
     1081 taagttccgg acaccgccag cctgtttttc tgaatacggg ctggtatgag tctgtgtctg
     1141 ttcatcaaat ctcgtttttt tttggtagac gcaagaatca gtctgattga taagtttaca
     1201 gatccatttt aggagaaaaa atgagaatct ggtcagcaat ttttctgttt atatttctgt
     1261 cgtatcctgt attaagttca gagaatgcag tcaaggggtt tattgatttg agttctttca
     1321 att
//
LOCUS       DAOQGJ010001422         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81234_length_1322_cov_4.367009, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001422 DAOQGJ010000000
VERSION     DAOQGJ010001422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81234_length_1322_cov_4.367009"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PK683_18395"
     CDS             <1..555
                     /locus_tag="PK683_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10478.1"
                     /translation="DGLFAFMILGTSALKFILKDYGRTPALLLLAFSLFDIYFIKIRG
                     EFINPFIEPITDIQRIGNGKTENCLFNGKTASLQNRTFETVSEFNGSKSFFTLRQTKS
                     FLNTGNVNMFALSETIDEKGFFKDRFGNSFVWDSEYNENEMILSALNLTSLCTLKFRE
                     PEFHKGKWMKQITFPKPLMEKNRP"
     gene            552..>1322
                     /locus_tag="PK683_18400"
     CDS             552..>1322
                     /locus_tag="PK683_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10479.1"
                     /translation="MNSKLTAGILFFLLQISALFLEEFRFSLFFEMFDADAVFLLFLF
                     GFFKYRAKTVEKGLYLLNSLILVSYSSYKGVLFLHETEIPRLSLYFKVYFLFSLIFYL
                     YIILKMKSERTLFWATLILSFSIFNLNYFFSLSFRTVPHLFYVIYSIHYFRKKNDKSK
                     RLALPDISVIFLVFSASLSAFLGVDIFRSVSALSSILCAAGAYFLASSSTANEKKTVQ
                     LLFVFQFLSTTAVFLLFIIIRHLFGTVPADDRYLAGFHV"
BASE COUNT          358 a          273 c          227 g          464 t
ORIGIN      
        1 gacggcctct ttgcattcat gattctgggt acatctgcgt taaagtttat tcttaaggat
       61 tacggaagga cccctgccct gcttcttctc gctttctctc tttttgacat ttatttcata
      121 aagatcagag gggagttcat aaatcctttc attgaaccga ttacagacat tcaaagaatt
      181 ggaaacggca aaactgagaa ctgtctgttt aacggaaaaa ctgcttccct gcagaacaga
      241 acctttgaga cagtgtccga atttaacggc tctaaatcat tttttacact caggcagaca
      301 aagtcattcc tgaataccgg aaatgtcaat atgtttgctc tttctgaaac gattgatgaa
      361 aaaggttttt tcaaagaccg tttcggaaat tcatttgtat gggattcaga atataatgaa
      421 aatgaaatga tactctcagc gttaaatctg acatctttat gcacactgaa atttagagaa
      481 cctgaattcc acaaggggaa atggatgaag caaataacct ttccgaagcc cttaatggag
      541 aaaaaccgac catgaacagc aaattgacgg ccggaattct atttttcctg ttgcagatct
      601 cagctctttt ccttgaagaa ttcagattca gcctcttctt tgaaatgttt gatgctgatg
      661 ctgttttcct tctttttctt ttcggctttt ttaagtacag ggcaaagaca gtggaaaaag
      721 gtctttatct tctgaattct ctgatccttg tatcttacag ctcgtacaaa ggagttctct
      781 tcctccacga aacagaaatt cccaggctga gtctttattt caaagtatac tttcttttta
      841 gtctgatttt ctatctttac attattctga aaatgaaatc agaaagaacc ctgttctggg
      901 caactctgat tctttctttt tccatattca atcttaatta ttttttcagt ctgtcttttc
      961 gtacagtccc tcatcttttt tatgtgattt attcgataca ctatttcagg aagaagaacg
     1021 ataagtcaaa acgacttgca cttccagaca tttcagtcat ctttctcgta ttttctgcat
     1081 ctttgtctgc attcttaggt gtagacattt tcagatctgt ttcagcactc agttcaattt
     1141 tatgcgcagc cggtgcatac tttcttgcat cttcctcaac cgcaaatgaa aagaaaacag
     1201 ttcagctgct ctttgtcttt cagtttctgt ctacgactgc agtcttttta cttttcatta
     1261 ttatcagaca tctgttcggg acagttccgg ccgatgacag gtatcttgca ggatttcatg
     1321 tt
//
LOCUS       DAOQGJ010001423         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81248_length_1322_cov_3.346488, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001423 DAOQGJ010000000
VERSION     DAOQGJ010001423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81248_length_1322_cov_3.346488"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..785)
                     /locus_tag="PK683_18405"
     CDS             complement(186..785)
                     /locus_tag="PK683_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH10480.1"
                     /translation="MTDPYENFYRVLNAFSGISFPEFERVKHLFHKRKIPKDAFFIRS
                     GEKPVSFAFVSSGLFRSFYCTDAGEEYTKNFVKLGEIIAAYSSYLAGTDSSINIEALK
                     DSLILDCKFKEFTVLTESSLPWERFRRKLSDLLFIQREIREFELLTLDAADRYRKFLE
                     KFSEIENEITDYNIASYLGITPVSFSRIRKKIRNNDKSN"
     gene            1227..>1322
                     /locus_tag="PK683_18410"
     CDS             1227..>1322
                     /locus_tag="PK683_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNH10481.1"
                     /translation="MKIKTPISEMLRIDLPIIGAPMFLVSYPELVA"
BASE COUNT          409 a          265 c          256 g          392 t
ORIGIN      
        1 gacaaggata cggggaatga ttgcgggctt ttccataact tcattactac tgatacagaa
       61 caggcaattc ctgtgatccg tgaattttta aaaaaagggt gactgttcag tccagtatct
      121 tcgcagccgc tttttttgca gtgccctccg gcggcttcag gacttcgcca ggaacctgaa
      181 ataagctaat ttgacttgtc attatttctt attttttttc taattcgact gaatgaaacc
      241 ggggtaattc ctaaatatga ggcaatattg taatcagtaa tttcgttctc gatttcgctg
      301 aatttttcca ggaatttcct gtatcgatct gctgcatcaa gtgtgaggag ttcgaattct
      361 ctgatctccc tttggatgaa aagcagatca gaaagttttc tgcgaaatct ttcccatgga
      421 agactggact ccgtaagcac agtaaattcc ttaaacttac agtccaaaat caacgagtct
      481 ttcagtgcct caatattgat gctggaatca gttccggcca gataagaact gtaagcagca
      541 atgatttcac caagctttac aaaattcttt gtatattctt caccggcatc agtgcagtaa
      601 aaacttctga aaagtccgga agagacaaat gcgaaagaaa caggtttttc accggagcgg
      661 ataaaaaaag catctttggg aattttccgc ttatgaaaca aatgcttcac tctttcaaat
      721 tcaggaaaag aaattcctga aaaagcattt aagactctat aaaaattttc ataaggatct
      781 gtcataggct tttctttccc gctctgaatt taccctactg caaaaaaata tgctgtattt
      841 tattttgcaa gtctaagttc ccagatttgg aacagcgaaa caaggttgaa cctatccgtc
      901 gaatatggcc tgtgataaaa ctaatgcgac tttgtcaagt cgcagtaaat agagttttcg
      961 gcagttcggc gaaagctggg atccacgccg gcctgctcaa ggtcgatcag aattcaaaac
     1021 ggctatggag gaactgattg actaactgca tccgacttca tcagaaatct gagcagcctg
     1081 aattagataa aattcatctg cagaaaaaat taagccttga ccgaaaaacc actgttaagg
     1141 aattttagcc gtataaactt aatgagtctg aatttctgag catatatttt ttataagccg
     1201 aaaaacattg catacaggat aagattatga aaataaagac accgatctcg gaaatgcttc
     1261 gaattgatct gcctataata ggtgcaccta tgtttctggt ctcctatccc gaacttgtgg
     1321 ct
//
LOCUS       DAOQGJ010001424         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81258_length_1322_cov_2.998421, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001424 DAOQGJ010000000
VERSION     DAOQGJ010001424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81258_length_1322_cov_2.998421"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PK683_18415"
     CDS             complement(<1..869)
                     /locus_tag="PK683_18415"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNH10482.1"
                     /translation="MFLRLSLLLFIPLSFLFSQDSVSPPAKSSDADRIILSVEYFNPL
                     DLSKEMYSVSLKTPRTNELIEDLKGTSKKKYKFTVGGIQSDIVPSSVKKDKDDDSLVN
                     LNITAYNVIPSGESVLKITVAGNEIISHTVQIPSRNSTPGKQPLIHSITPEAGRKGDT
                     ITVKGENFGDDIDLISVVFGEYAKTDQGQTVEDFMERKPFYLANDGNKQELKFVLPVK
                     KDLLKDALYKRILSLHINVNGRPSDLKKLVILHDNWKFYVAGLTVFLSILFYFILAIA
                     FRNTPTCCCGYNVP"
     gene            complement(940..>1322)
                     /locus_tag="PK683_18420"
     CDS             complement(940..>1322)
                     /locus_tag="PK683_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10483.1"
                     /translation="LTSGKNSGIIQSKSKIKSQTKDHAKEDVQILEVFDYLGTKIVHE
                     NGVYTVNDRGKVTVYKSWIELPEKYQKMVRELDSRSVKSQSNTDYFLESINGNYFLTA
                     PGGKKTRYRSLSEIPAHIRKAIGK"
BASE COUNT          338 a          278 c          282 g          424 t
ORIGIN      
        1 ggaacattgt atccgcagca acaggtagga gtgtttctga atgcaattgc caggatgaaa
       61 taaaacagga tggataaaaa tactgtcagc cctgcgacat agaatttcca gttgtcatgg
      121 aggatgacca gttttttcag atcggaagga cgtccgttca cattgatatg aagagacagt
      181 atccttttgt aaagtgcgtc tttcagaaga tcctttttta cgggaagaac aaatttgagt
      241 tcctgcttgt ttccgtcatt cgcaagataa aacggctttc tttccataaa gtcttccact
      301 gtctgtccct gatcggtctt tgcatattct ccgaacacaa cggaaatcag gtctatatca
      361 tctccgaagt tttcgccttt gactgtgatc gtgtcccctt tccttcctgc ctcaggagtg
      421 atggaatgga tcagaggctg cttgccggga gtggaatttc gggatgggat ctgcactgta
      481 tgagatataa tttcatttcc cgcaacggta attttcagga cagactcccc tgaaggaatg
      541 acattatagg cagtgatatt caggttgact agactgtcgt catccttgtc ttttttaact
      601 gaggacggta caatatccga ctgaattccc ccgacggtga acttgtattt ctttttggag
      661 gtccctttca gatcctctat cagttcattc gttctgggtg ttttcaagga tactgaatac
      721 atttccttgg aaagatccag aggattgaaa tattccacac tcagtatgat acggtcggca
      781 tccgatgatt ttgcaggcgg agaaacagaa tcctgtgaaa acaggaaact taacggaatg
      841 aagagaagca gtgacagtct taaaaacata aattcctcta tgaaactcta gtgaatatcg
      901 gctatgtccg tctgaaaaat tactttgagg gaaaaactgc tattttccta ttgcctttct
      961 gatatgtgca ggaatttctg acaggcttct gtatcttgtc ttttttccgc ccggggcagt
     1021 tagaaaataa tttccattta tgctttccag aaaataatct gtgtttgact gtgattttac
     1081 cgagcggctg tccagctctc tgaccatttt ctggtatttt tccggaagct caatccagga
     1141 cttgtacact gtgacctttc ccctgtcatt gactgtgtat accccgtttt catgcactat
     1201 cttcgtgccc agataatcaa atacttcaag tatctgcaca tcttcctttg catggtcctt
     1261 agtctgagac ttgatcttgg atttgctttg aataatgccg ctgttttttc cggaggtaag
     1321 aa
//
LOCUS       DAOQGJ010001425         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81261_length_1322_cov_2.869771, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001425 DAOQGJ010000000
VERSION     DAOQGJ010001425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81261_length_1322_cov_2.869771"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PK683_18425"
     CDS             <1..1285
                     /locus_tag="PK683_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10484.1"
                     /translation="NKKKPFLLSARIRMGMQKNIHGEIMEDLKSLEKSARYSELIVKI
                     RELIRENPRNLENEVIKGFMGRRWRNIFISEASADSNAVAAAEENKLFGKDVRSDREK
                     IASRFLKGEGPEEWQTEMPAPENRPVKNTTLIFCPGLLTGLLPVLAFRQEFPLISKKF
                     SLKIVQSDSHPMRGCEANMQDLLKAVEKGEGLDESAEPIPENSALPPKDIFVIAYSKG
                     MSDLLFLLSRRPDLKDRIRCIFSWAGAPGGSYLANSMYEAAKNIHLDSKINPEQLEGI
                     LKLVSPLVNLPSQIRRLPEYDIKGALLDLCTYHRKEFLEENLKKIDELNIPIFNITGS
                     TSVTEVPYFQIQGYMDLLKYDANNDMQVTQMHSKVQSPMATDLAMLHGHHWDLSYGPF
                     PRAMRFGSPNLEHKFPREAALSSIIKFALEFGLID"
BASE COUNT          401 a          272 c          308 g          341 t
ORIGIN      
        1 gaataaaaaa aagccttttt tattatccgc gagaataaga atgggaatgc aaaaaaatat
       61 tcacggggaa attatggaag acttgaaatc attggaaaag agtgccaggt attcggaact
      121 gattgtaaaa atcagggagc tgatcagaga aaatccgagg aatctggaaa atgaagtgat
      181 caaaggtttt atgggacgca gatggagaaa tatttttatt tctgaagcat cggcagattc
      241 gaatgcagtg gcggcagcag aagagaataa acttttcgga aaggatgtcc gttccgacag
      301 agaaaaaatt gcatcccgat ttctgaaagg agagggtccg gaggaatggc agacggagat
      361 gcctgctccg gaaaacagac ccgtaaaaaa tacgactctg attttctgcc cgggacttct
      421 gaccggactt cttcctgtgc tggcattcag gcaggaattt cccctgatct caaaaaagtt
      481 ttccttgaaa atcgtgcagt ctgactccca tccaatgaga ggctgtgagg cgaatatgca
      541 ggatctgctg aaagcagtgg agaagggtga gggactcgat gaatcagcag aacccatccc
      601 ggagaacagc gccctgcctc cgaaagatat ttttgtgatt gcgtacagca agggaatgtc
      661 tgatctgctg tttcttcttt cgagacgccc cgatctcaag gacaggatca gatgcatatt
      721 cagctgggca ggagcaccag gaggatctta tctcgccaat tcaatgtatg aggcggccaa
      781 gaatattcac ctggacagta agatcaatcc cgaacagctg gagggaattc tcaaacttgt
      841 ttcccctctg gtaaatcttc cttcccagat cagaagactt cctgaatacg atatcaaagg
      901 cgcgcttctg gatctgtgca catatcacag aaaggaattc ctggaagaaa atctgaaaaa
      961 gattgatgaa ctgaacattc cgatattcaa catcaccgga tcaacttcgg ttacagaggt
     1021 tccttatttt cagattcaag gttatatgga tcttctgaaa tatgatgcaa acaatgatat
     1081 gcaggttact cagatgcatt ccaaagtgca gtctcctatg gcaactgatc tggcaatgct
     1141 tcacggtcat cactgggatc tttcctatgg acccttcccc agggcaatga gatttggttc
     1201 acctaatctg gagcacaaat ttcccagaga agcagcactt tcttccatca ttaaatttgc
     1261 attagaattc ggtctgattg attaatcatt gcccagatat tcattgactg attttcttga
     1321 tt
//
LOCUS       DAOQGJ010001426         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81491_length_1320_cov_2.207115, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001426 DAOQGJ010000000
VERSION     DAOQGJ010001426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81491_length_1320_cov_2.207115"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..812
                     /locus_tag="PK683_18430"
     CDS             69..812
                     /locus_tag="PK683_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH10485.1"
                     /translation="MKNKKTVLILAATSDIGKHIAFGFAERGWDLYITGKDPIALNQV
                     TSILENKYAAYVQEFILDITDYPSHRGFLDSLPELPDTVVCCFGYYKDQEKALSDFQE
                     AYLTMSVNYVGAVSFLNLVSDKFQKRGYGSIGVISSVAGERGRQMNFLYGSAKAGLTV
                     YLSGLRNRLFKSNVSVTTILLGPVYTRMSEGHNLMPVLTAKPEQAAEEIIHAVIKKKD
                     NVYILWYWRFIMMIIRSVPEFIFKRLGKF"
     gene            complement(862..>1320)
                     /locus_tag="PK683_18435"
     CDS             complement(862..>1320)
                     /locus_tag="PK683_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10486.1"
                     /translation="FDDGMERKILLEPDLLAEFGAGSSGKTHSRSSEPQSSKGGVKIW
                     NISGSSLSRNLSDGKMTDLSGETSPVFSENGRLLAFPGGVLVTLKNGKSCQSWAAGKN
                     LRLGEQSAIPNLCNIITVSGNASFETANSLRMDPDVKSAVPNLWMESDTR"
BASE COUNT          380 a          285 c          298 g          357 t
ORIGIN      
        1 gaaatgccgg atttctctga tgctcttttg aaattatgcc tgcaataaat atatgaggat
       61 acattataat gaaaaataaa aaaactgttc tgattctggc agcaacctcg gacatcggaa
      121 aacatattgc tttcgggttt gcagaaagag gctgggatct ttatatcacc ggaaaagacc
      181 ccatagcatt aaatcaggtt acttccatac tggaaaataa atacgcggca tacgtgcagg
      241 aattcattct ggatattact gattatcctt ctcacagggg attccttgat tcactgccgg
      301 aactgccgga cacagttgtg tgctgtttcg gatactacaa ggatcaggaa aaggcactct
      361 ctgattttca ggaagcctat cttacgatga gcgtgaacta tgtaggtgct gtttccttcc
      421 tgaatcttgt atcagacaaa ttccagaaaa gaggttacgg atcgataggt gtcatcagct
      481 ctgtggcagg agaacgagga aggcagatga actttctgta cggaagtgca aaagccggtc
      541 tcactgtata tctttctgga ctgcgaaaca ggctgttcaa aagcaatgtc tcggtgacaa
      601 ccatccttct tgggcctgtc tatacaagaa tgtccgaagg acataatctg atgccggtac
      661 ttacggcaaa gccggaacag gcggctgagg agattattca tgccgtcatt aaaaagaaag
      721 acaatgtata tattttatgg tactggagat tcattatgat gatcatcaga tcagtacctg
      781 agttcatatt caagaggctg gggaagttct gagaccggat ttccggaact cagaactttt
      841 gatatccttc agaacgaaag ttcagcgggt atccgactcc atccagagat taggaacggc
      901 ggactttaca tccggatcca ttctcagaga atttgcagtc tcaaaggaag catttccgga
      961 cactgtaatg atattgcaga gattcgggat tgcagactgt tcgccgagtc tcaggttttt
     1021 tcctgctgcc caggactgac aggattttcc attcttcaga gtgacaagaa cccctccagg
     1081 aaaagcaaga agtctcccgt tttctgaaaa cacaggagag gtttcaccgc tcagatcagt
     1141 cattttccca tctgaaagat ttctggaaag agaggatcct gaaatattcc agattttcac
     1201 tccgcctttc gaagactgcg gctctgaaga ccttgaatga gttttaccgg aactgcctgc
     1261 tccaaattct gccagaagat ccggctcaag cagaatcttt ctttccatcc cgtcatcaaa
//
LOCUS       DAOQGJ010001427         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81505_length_1320_cov_1.865613, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001427 DAOQGJ010000000
VERSION     DAOQGJ010001427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81505_length_1320_cov_1.865613"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PK683_18440"
     CDS             complement(<1..1147)
                     /locus_tag="PK683_18440"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNH10487.1"
                     /translation="MKNTIKFALIICSLGFLADCASKKKGFLSQAIPLALLGTNTGGN
                     KNIPAKIDAPSEFSYSLSGDNSNGQINTNIAETDQPVNFTPSISGTKPITFSISPALP
                     KGLVIDSETGVISGTPEIWSLSTEYTITAQNPSGTVSVKITFSTTTPGRVTGTGINKC
                     YSWPVNQEIPCGDSTYPNQDADFQTPRSFTGPTPHTVYTDDYTTRDNVKGLVWKSCLE
                     GQSGSDCSGGNGPYIFTRESAVAACNNLNTANAGNGYAGMQTWRVPTIQELYTIFDNS
                     VYYGVPKSSPAYFPGNDGYTKFYISSSIFNESRLWIFSDTTKQEHSYPVLSMFVLRCV
                     SGQSLPIPSFRDNQDGTVGDHTSKLLWQKCPAGLSGSNCESGSLNRFS"
BASE COUNT          392 a          267 c          256 g          405 t
ORIGIN      
        1 aagaaaaacg atttaggctt cccgattcac aattagagcc tgataatcct gcaggacact
       61 tttgccataa aagcttactt gtgtgatctc ctaccgtacc atcctgatta tccctaaatg
      121 aaggtattgg cagagattga ccagatacac accgtaatac aaacatagat agtacaggat
      181 agctatgctc ctgctttgta gtatcactaa atatccatag tctggattcg ttaaaaatgc
      241 tggaagaaat ataaaactta gtatacccgt catttcccgg gaaataagcg ggagaactct
      301 taggtacacc gtagtataca gaattgtcaa atatagtata tagttcctgt attgtcggaa
      361 ctctccaagt ctgcattcct gcatacccat ttcctgcatt agctgtgttc aaattattac
      421 aggcagcaac ggcactttct cgggtaaaaa tatagggacc atttcctccc gaacagtctg
      481 accctgactg cccttccaga cagcttttcc aaactagacc ttttacattg tctcgcgttg
      541 tataatcatc agtatagaca gtatggggtg ttggtccagt aaaacttctg ggggtttgaa
      601 aatcagcgtc ctgattagga tatgttgaat ctccgcaggg aatttcctga tttactggcc
      661 agctataaca cttattaatt cctgttccag tcactcttcc aggtgtcgtt gttgagaagg
      721 taatctttac tgaaactgtt ccactagggt tttgggctgt gatcgtgtat tccgtagaaa
      781 gactccatat ttcaggagtt ccagatatca cacccgtttc cgaatcgata acaagtccct
      841 taggcaaggc aggactgata ctgaatgtaa tgggctttgt cccgctaatg gaaggagtaa
      901 aattcaccgg ctgatcggtc tcagctatat tagtatttat ttgcccgttt gaattgtctc
      961 cggaaaggga ataagagaat tcacttggag catcgatttt tgccgggata tttttattcc
     1021 ctccagtatt cgtaccaagc aaagctaagg gtatagcctg agaaagaaaa cctttctttt
     1081 tactggcaca gtcagccaaa aaaccaagag agcaaataat gagggcaaat ttgattgtat
     1141 ttttcatgtt ttttccattt tactttagct tcttatgtca gatgcgtaaa atacaagaat
     1201 atagtcaaat ctttttttga ctttattcgg cttagatgaa cctttactaa tgacataaat
     1261 cagcaacaag gaaacagatt tactgcatac cgaatttttt cagtaagatt aaaaaaggaa
//
LOCUS       DAOQGJ010001428         1319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81549_length_1319_cov_3.425633, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001428 DAOQGJ010000000
VERSION     DAOQGJ010001428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81549_length_1319_cov_3.425633"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..888
                     /locus_tag="PK683_18445"
     CDS             124..888
                     /locus_tag="PK683_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10488.1"
                     /translation="MKSRKETAGAVIFCIVNGILAMQCLTIHVQNRIVGSEKDSERTF
                     PVMEKSGTAAKNPWIELGTVNRIFFPFENGKLFCADLKPELFDVTLFEENDCSVNSSA
                     QTVDAEFIYFPGKFQTDEKLKILYSPEDRHNLLAAAYRKKTVSIFDWGNKTSYALREL
                     FISDSDEKLNQLGKQYTVIIIMDTKNTGLYGIVPKKPVFSQSSFVHAPWLSSKTENSA
                     SLWRILEIPAFVTDIITFPIQFVGGWIYIMLFGFKK"
     gene            complement(936..>1319)
                     /locus_tag="PK683_18450"
     CDS             complement(936..>1319)
                     /locus_tag="PK683_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10489.1"
                     /translation="LLAFVSYCGGDKDASKEADVKKDAPEMKSAAKTEAPKENCDFNC
                     SELGDEATAFYNTKGEWAGTFTMKVQAIKQKAEGVCHIKYEYVSVPGKNQPSGIDMRE
                     FKYAKNGCKWNVTSMGPNMSAKASF"
BASE COUNT          368 a          261 c          290 g          400 t
ORIGIN      
        1 ttctgtttga tgcagtgacg tatccggttc aggttgtcat agtatttatc tacattgcat
       61 tttttggact gaaaaaatga tggatattca gtctttgcaa ttcagatatt ttggcggaga
      121 actgtgaaat ccagaaaaga aacagcggga gcagtgatat tctgtattgt aaacgggata
      181 ttggcaatgc agtgtctaac aattcatgtt cagaacagga tcgtagggtc ggaaaaagac
      241 tcagaaagaa cgtttccagt tatggaaaaa tcgggaacgg cagcaaagaa tccttggatt
      301 gaactcggca ctgtcaacag aatttttttt ccatttgaga acggaaagct tttctgtgcc
      361 gatctgaaac cggaactgtt tgatgttacc ctgttcgaag aaaacgactg ttccgtgaac
      421 agcagtgccc agaccgttga tgcagagttt atttactttc ccggaaaatt tcagacagat
      481 gaaaaactga agatccttta cagtccggag gacagacata atcttcttgc tgctgcttac
      541 agaaagaaga ctgtctctat tttcgactgg gggaataaga cttcctatgc actgagggaa
      601 cttttcatat cagactcgga cgaaaagctg aatcagctgg gaaaacagta tacagtgatt
      661 ataataatgg atactaaaaa tacaggtctc tatggaattg tacccaagaa gccggtcttc
      721 agtcagagca gctttgtgca tgctccttgg ctttcctcta aaaccgaaaa ttcagcaagc
      781 ctatggagaa ttttggaaat accggcattt gtcactgaca ttattacttt tccgattcag
      841 tttgtcggag gctggattta cattatgctt ttcggattta aaaagtaaac tgaaactgta
      901 ctgtatggac agtacagtct gacgggagat gaagatcaga aagaagcctt tgcggacata
      961 ttcggtccca ttgaggttac attccacttg catccgttct ttgcatattt gaattctctc
     1021 atatcaatcc ctgagggctg attttttcca ggaacggaaa catattcata tttgatatgg
     1081 catactcctt cagccttctg cttgatcgcc tgcactttca ttgtgaaagt tcctgcccat
     1141 tcgccttttg tattgtagaa ggctgtcgct tcatctccaa gttcactgca gttgaaatcg
     1201 cagttttctt tcggagcttc agtttttgct gcagacttca tctcaggagc atcttttttg
     1261 acgtctgctt ctttggaagc atctttatca ccgccgcagt aggaaacgaa ggcgagcag
//
LOCUS       DAOQGJ010001429         1319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81611_length_1319_cov_1.916139, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001429 DAOQGJ010000000
VERSION     DAOQGJ010001429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81611_length_1319_cov_1.916139"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PK683_18455"
     CDS             <1..919
                     /locus_tag="PK683_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10490.1"
                     /translation="RWLLDTWAGTSDGVPFAIPGASEDIQTVYTDEVKFTPILSPVWE
                     TYDSENSGTIFLRAKIAGDGLFPPDELSFANWGYAFTTVWDYPVDSNRAVTGDSAILA
                     LWKEKTVTAGSTHTSAMEFSYVEKSPGLHFTLDDPKKGRGILEIQYQNDTNADQELIY
                     ELKSSAAIISDNDSLRFKYNIPAKSKLSRTVLLNFQGHGDQSLTVSETLSGTVKNYTY
                     PFKLSGINEGNSPPVWTSGKKYPITYLHGSPDLKLTGIIKNPNTGKVISSGDMLPKKQ
                     NDGNYLYECELDSRDYIGQVEVEIQEKEL"
     gene            complement(926..1165)
                     /locus_tag="PK683_18460"
     CDS             complement(926..1165)
                     /locus_tag="PK683_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10491.1"
                     /translation="MKIRTEKGNYIITGDECYLPKECEEGSGLNPEASFSVKNNRHFI
                     QNHILKERNQTVLTMHDPEIEKKGKKDSSGFVRIF"
BASE COUNT          398 a          286 c          295 g          340 t
ORIGIN      
        1 aagatggctc cttgacacct gggcgggaac aagcgacggt gtaccttttg ccattcccgg
       61 tgcatcggaa gatatacaga ccgtatacac tgacgaagtg aagttcactc ctatcctgag
      121 cccagtctgg gagacatatg acagcgaaaa ttcaggaact attttcctcc gtgccaaaat
      181 agcaggtgac ggtcttttcc ctcctgatga actgagtttt gcgaactggg gctatgcatt
      241 caccactgtc tgggactatc cggtagattc aaacagggct gtgacagggg attctgcaat
      301 ccttgcactc tggaaggaga aaacagtcac ggctggatca acacatactt cggcaatgga
      361 gttttcatac gttgaaaaaa gcccaggact tcattttact cttgatgacc ccaaaaaagg
      421 aagaggaatt ctggaaattc agtatcagaa cgacacaaat gcagatcagg aacttatata
      481 tgagctgaag tcaagtgcgg caataatttc cgacaatgat tcattaagat ttaaatacaa
      541 tatacctgca aagtcaaaac tgagcagaac tgtactcctg aattttcagg gtcacggaga
      601 ccaatctctt accgtttcag agacactttc aggaacggtg aagaactaca catatccgtt
      661 taagctgtca ggaatcaatg aaggcaattc tcctcctgta tggacctcag gaaaaaaata
      721 tccgatcact tatctgcacg gaagcccgga tctgaagctg acaggtatca tcaaaaatcc
      781 aaatacaggg aaagtaatat cctcaggaga tatgcttccc aaaaagcaga atgacggaaa
      841 ctatctctac gaatgcgagc ttgactccag ggattatatc ggacaggtag aagttgaaat
      901 tcaggaaaag gaactgtgaa aaaatctaaa agattctgac aaatccggaa gaatcttttt
      961 ttcctttttt ttcaatttcg ggatcgtgca ttgtaaggac agtctgattt ctttccttaa
     1021 gtatatgatt ctgtataaaa tgtctgttgt tctttactga aaaggaagct tccggattca
     1081 gtccgctccc ctcttcacat tccttgggaa gatagcactc atctcctgtg ataatataat
     1141 ttcctttttc tgttctgatt ttcaatgcct gagaccctgt tgtatgaccg gcgaggatca
     1201 cggcagcgat tggctgggag ccgaacagga actcctgcca ggaaaagttc aggacgtcgg
     1261 cgagcgcgtc gaacatctgc ttaaaaatgt cgaaggccac gatgaaagga aggccggct
//
LOCUS       DAOQGJ010001430         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81638_length_1318_cov_4.285827, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001430 DAOQGJ010000000
VERSION     DAOQGJ010001430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81638_length_1318_cov_4.285827"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..991)
                     /locus_tag="PK683_18465"
     CDS             complement(248..991)
                     /locus_tag="PK683_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10492.1"
                     /translation="MEKSIIKLFILSFIVSLASALSANPFKGTWINNPVYSGEYGKMA
                     IVGDNTVEVGFWGPGGQTMEASYTVQGDSLKYTITKARYMQEKDNGKKGVCKIVKLKD
                     NPYFQEELNCTGVIGTYLNSSGNDDVPKVFRTFLSKDTARKIQGNDTLSILKEGTVND
                     TAYLRSAPNASAKPLDCTIYDQSGKGHKSQVIPKDRKLLVIAKTKTKFKVQKWENHWY
                     FVDAGGPSGYDSVNNCGPGWVFGEFVTIK"
BASE COUNT          374 a          275 c          270 g          399 t
ORIGIN      
        1 tgtaaaaatt ccacagagac tccgcagtat atggaagaac tgacaatgga ggcgtatgac
       61 tgcaaaagca aagacggatc agttgcaaaa gtcatcaaat tcataggtgc tgataaagag
      121 tctgacgttc tgtacggagt atacaaataa gttaatccga agatatgaaa aataatttat
      181 atcccaagaa tgtctgccca catcttggga tataattaca ttaaactccg aaaaagactg
      241 cgaggtctta ttttattgta acaaattctc cgaacaccca ccccggtccg caattattta
      301 cagagtcata accgcttggg ccgcctgcat ctacaaagta ccaatggttt tcccatttct
      361 gcaccttaaa tttagttttt gttttggcaa tgactaataa tttccggtcc ttgggaataa
      421 cctggctttt atgtcctttt cctgactgat cataaatggt gcaatccaaa ggttttgctg
      481 aagcattcgg agcagacctg agataagcag tgtcattgac tgtcccctct ttcagaatag
      541 agagggtatc gtttccttga atttttctag cagtatcctt ggaaagaaaa gtgcgaaaaa
      601 cttttggtac atcatcattg ccgctggaat tcagataagt tccgatcact cctgtacagt
      661 tcaactcctc ctgaaaataa ggattgtctt tgagttttac aatcttgcag actccttttt
      721 ttccgttgtc tttttcctgc atatacctgg cttttgtaat cgtatatttt agactgtcac
      781 cctgaactgt atacgatgct tccatggttt gcccgccagg accccaaaag ccgacttcaa
      841 cagtattatc tcctacgatt gccatcttgc cgtattcgcc gctgtataca gggttgttga
      901 tccaagtgcc cttgaacgga tttgcagaaa gggcagatgc gagagaaact ataaaactta
      961 atataaagag tttgattatc gatttttcca ttgggtcata gtatttcatt tttcaaaaat
     1021 gtcaatagga attatgccgc caactcttcg actagacggg agacagttcc cggaatcagg
     1081 ataaaagact gaatcgatgt tttcaaggcg cgtgagttga tttcgctggg tggatcgtaa
     1141 tgggcgaatg gcaggctgct ctgctttttc ttcatcgctt taggtgaatt gcctgttaac
     1201 caagagagct gatacggcca atcgtgttct aaaaccagcc ctcgcatcgc agtagggaca
     1261 gggcgctgca gattctgttt attatatgcc tgaggaaaaa aacaatctta atcatata
//
LOCUS       DAOQGJ010001431         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81762_length_1317_cov_3.977813, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001431 DAOQGJ010000000
VERSION     DAOQGJ010001431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81762_length_1317_cov_3.977813"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PK683_18470"
     CDS             complement(<1..1286)
                     /locus_tag="PK683_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10493.1"
                     /translation="MYEKRFVLTIPIRTYLSSTDVSATAGVPFNDAIFVIVVANGLKG
                     LMSAKPFCGFVKCDALDNGKWLLANQPLKTDYTQQGALTFFSSVPGDSVIQAEVWGYG
                     VQPLAGGTFSFLDVNVFSTIYNSILYTKATQPDQFYGPIPPVISQQFSALELLGFLKD
                     NRTLSNAVTPSVVGANWDKRNRLLPFIAALSRTLYEQTDRASSKNAFEYLGRLTDILL
                     RPFVLQAQEPLARTADPTVPEFKLYKIRGATDIAGTGYSMRSPNLGTSDYYPHTLELE
                     NSDTRSLLSFLIEDSRRQSDGILALLAKRNIFSSGVSLLSELGRNERTSGRQTAVAAI
                     QGILPEIEVSVWDRTNLANVIEAYPSNPVRFNLQESINSAEYDIIHYTDSRNADITHS
                     SWDEINDVTDFLYTYLSPESKYTVSRNISAFFDVIIA"
BASE COUNT          371 a          289 c          291 g          366 t
ORIGIN      
        1 gcaatgatca catcaaagaa tgcacttata ttacgggata cagtatattt tgattcaggt
       61 gaaagataag tataaagaaa atctgtcaca tcattgattt catcccagct tgaatgcgta
      121 atgtctgcgt tcctgctgtc agtataatgt atgatgtcgt attctgcaga attgattgat
      181 tcctgcaggt tgaaacggac agggttcgaa ggatatgcct caatgacatt tgccagattg
      241 gtcctgtccc agacggaaac ttcaatttca ggaagaatac cctgtattgc tgccactgca
      301 gtctgtcttc ccgatgttct ttcatttctt cccagctcag agagcagtga cactccgctg
      361 gaaaagatat tcctttttgc aagcagagca agaattccgt ctgactgacg tcggctgtct
      421 tcaatcagaa aagaaagcag cgagcgggta tctgaattct ccagttccaa tgtatgaggg
      481 taataatcag aggttcccaa attcggactt ctcatactgt agccagtgcc tgcaatgtca
      541 gtagcgcctc ttattttata cagcttgaat tcaggaactg tcggatctgc agttctggca
      601 agaggctcct gtgcctgaag aacaaaagga cgaagaagaa tatctgtcag tctgcctaga
      661 tattcaaatg cattcttgct ggaagctctg tctgtctgtt cataaagtgt ccttgacagt
      721 gccgctataa aaggaagaag acggtttctt ttgtcccaat tggcaccgac aactgaagga
      781 gtgactgcgt ttgagagagt tcggttgtcc tttaaaaagc caagcagttc aagggcagaa
      841 aactgctgag atatgactgg aggtataggc ccataaaact gatccggctg tgttgcttta
      901 gtatataata tactgttata tattgtagaa aaaacattca catccaggaa actgaaagtt
      961 ccccctgcaa gcggctgcac accgtatccc catacctcag cctgaatgac tgagtcgccc
     1021 ggtacgcttg aaaagaaagt cagggctccc tgctgagtgt aatccgtctt aagaggctga
     1081 tttgccagaa gccatttccc gttgtccaag gcatcgcact ttacaaatcc gcagaatggt
     1141 ttcgcggaca tcagtccttt caaaccgtta gccacaacga tgacgaaaat tgcatcatta
     1201 aaaggcactc ctgctgttgc agatacatct gtagaactga gataggtgcg gatcggtatc
     1261 gtcagtacaa atcttttttc atacatcagc cattgaaaat ttctgtacat agcctct
//
LOCUS       DAOQGJ010001432         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81774_length_1317_cov_3.485737, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001432 DAOQGJ010000000
VERSION     DAOQGJ010001432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81774_length_1317_cov_3.485737"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PK683_18475"
     CDS             complement(<1..575)
                     /locus_tag="PK683_18475"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH10494.1"
                     /translation="MEEWISNNSTAFRLSIFIGLFILFAVWEIVYPNHKPALERKYRW
                     ASNILLVVLNNALLRILFPITAIAFAETAVQNRWGFLNSLNLPAPASFILGILFLDFV
                     IYLQHSVFHSLPLLWRLHRMHHADLELDVTSGTRFHPIEIIISMFIKIAAIAVSGVSP
                     FTVLIFEVILNGMAMFNHSNIYIPEKADQIL"
     gene            complement(580..813)
                     /locus_tag="PK683_18480"
     CDS             complement(580..813)
                     /locus_tag="PK683_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10495.1"
                     /translation="MQYLSWLDNVSYPMLIFAAVLMGLAPFVPMPHLWEKILMLKNGD
                     LKKPIDIFDLFWHLVPVFILVLKVIRDRNSGQG"
     gene            complement(1036..>1317)
                     /locus_tag="PK683_18485"
     CDS             complement(1036..>1317)
                     /locus_tag="PK683_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10496.1"
                     /translation="GTIKWEGGALAANGKIYGIPFQSANVLVIDPSSDTASVFDSLGG
                     TNNWVGGVYASNGIIYGIPYNSTTVLAVDPKAIGVWTADMYLSPYFNKF"
BASE COUNT          434 a          283 c          268 g          332 t
ORIGIN      
        1 ctaagaatct gatctgcctt ctccggaatg tatatattgc tgtgattgaa catagccatc
       61 ccgttcagta tgacttcaaa aataagcaca gtgaaaggcg agactcctga tacagcaatt
      121 gcagcaattt ttataaacat agagataata atctcaatcg gatgaaatct ggttccgctg
      181 gtcacgtcaa gctccaggtc tgcatgatgc attctgtgaa gcctccagag cagtggaagg
      241 gaatgaaaaa ctgagtgctg aagataaatc acaaaatcca ggaaaagtat tcccagaata
      301 aatgaggcag gtgcagggag attcaaagaa ttcagaaatc cccatctgtt ttgaactgca
      361 gtctctgcaa aagcaattgc agttatggga aaaagaattc tgagaagtgc attattcaga
      421 acaaccaaaa ggatattgga agcccatctg tattttcttt ccaacgcagg cttatgattc
      481 ggatatacaa tctcccagac tgcaaaaaga ataaacagtc ctataaatat ggagagccta
      541 aatgctgtac tgttgtttga aatccattct tccatagaat cagccctgcc ctgaatttct
      601 gtcccgaatc actttcagaa caagaatgaa aaccggaacc agatgccaga aaagatcaaa
      661 aatatcgatc ggctttttca gatcaccgtt tttaagcatc agtatctttt cccaaagatg
      721 gggcattgga acaaaaggag caagtcccat caggactgcc gcaaaaatga gcataggata
      781 cgaaacattg tcaagccagg ataagtactg cataacaccc tccatgacca gaattgcaag
      841 cacgcattct gaaatcaatt cttttataca atagggggta tggtatatag gctgaaatcg
      901 aataaaagcg gaatttcaca aagaggatat gataagccct attgaaagaa tttccaaaat
      961 agcggagtta attcagtata ttatctgtgt tcatctcggc cataagcttt ctttgcagac
     1021 ccggcagggg aagactcaaa acttattaaa gtagggagac agatacatat ccgctgtcca
     1081 gacaccaatg gcctttggat caacggccag tacagttgtg gaattataag gaattccgta
     1141 gatgattccg ttcgatgcat ataccccgcc cacccagtta tttgttcccc ccagactgtc
     1201 aaacacggaa gctgtatctg aagaaggatc aattacaaga acatttgccg actgaaacgg
     1261 tatgccgtaa attttcccgt tggctgcaag tgcacctccc tcccacttga tcgtccc
//
LOCUS       DAOQGJ010001433         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81777_length_1317_cov_3.399366, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001433 DAOQGJ010000000
VERSION     DAOQGJ010001433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81777_length_1317_cov_3.399366"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PK683_18490"
     CDS             <1..545
                     /locus_tag="PK683_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HNH10497.1"
                     /translation="EIFPFLAKKPAAVSWDQESPTEVEKLEIEKAKERISEESSEIEK
                     LRNELQAEREKLDAEKVKLEEAKQGIKEKERQMQMKEKEDANRLSKLRALAGKISNMP
                     PKSAVELLVNWPDQDIIDVFKQMDRDAEEEGKASITNYLLTLFDPKRRSVITNKWLDS
                     ESDKVPEENINTLLEQEVVP"
     gene            545..>1317
                     /locus_tag="PK683_18495"
     CDS             545..>1317
                     /locus_tag="PK683_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10498.1"
                     /translation="MSNAKYNVEPGFFSAGIIQLVLNYNFLRYGIWEPSKIDLRMTPL
                     EILPDVKLLKNEVGFPLQNQEKSLVVGTVRMGYGHHRMAYALYTWGLKKYSQIYLHDL
                     IGLGSKESDAIKDVDGFYSQMSRAASEMGGMIEWSWGMLMSQGGISSLHTSLAFAKRY
                     VNLMGNISRDNPYISTYPLNGQIAVEAGVKKVIHLIPDNYPQYYLLVPGALNLVQSPA
                     AYLKFIEMGFPKENLSIAGHWVSEKIGANSIFHSECRLE"
BASE COUNT          424 a          240 c          328 g          325 t
ORIGIN      
        1 atgagatttt tcctttttta gccaagaagc cggcagcagt cagttgggat caggaatcac
       61 ctactgaggt ggaaaagctg gaaattgaga aggctaagga acgaatcagc gaagaatcct
      121 cggagattga aaaacttcgg aatgaacttc aggcagagag agaaaaactg gatgccgaaa
      181 aggtaaaact ggaagaggca aaacagggga tcaaggaaaa ggaaaggcag atgcagatga
      241 aggaaaaaga agatgccaac cgactttcca aattgagagc tttagccggg aaaatttcga
      301 atatgcctcc taaaagcgca gttgagctgt tggtcaactg gcctgatcag gatatcattg
      361 atgtattcaa gcagatggac agagatgcag aagaagaagg aaaggcatcg attacaaact
      421 atcttcttac tctgtttgat cccaaaagaa gatctgtaat tacaaataaa tggctcgatt
      481 cggaatccga caaggttcct gaagaaaata ttaatactct gcttgagcag gaagtggtac
      541 cataatgtca aatgcaaaat acaatgtaga accggggttt ttcagtgcag gaatcataca
      601 gcttgtgctg aattataatt ttttaagata tggaatctgg gagccttcaa aaatagatct
      661 ccgaatgact cctttggaaa ttctgcctga cgtcaaactt ctgaaaaatg aagtaggttt
      721 ccctttgcag aatcaggaga aatctctggt tgtaggaacc gtcagaatgg gatacggaca
      781 ccatcggatg gcctatgcac tgtatacctg gggactcaaa aaatattctc agatatatct
      841 tcatgatctg atcggtctgg gaagcaagga atcggatgcc atcaaggatg tggacggatt
      901 ctacagtcag atgagccgtg cagcttctga aatgggcgga atgattgaat ggagctgggg
      961 aatgctgatg tctcagggcg ggatttcttc tctgcatacc tcacttgcat ttgcaaaacg
     1021 ttatgtcaat ctgatgggaa atatttccag agacaatccc tacatttcca cttatccgct
     1081 gaatggacag attgcagtgg aagccggggt caaaaaagtg attcatctga ttcctgataa
     1141 ttaccctcag tactatctgc ttgtgccggg tgcattgaac ttagtgcaga gtcctgctgc
     1201 atatctgaaa ttcattgaga tgggatttcc caaagaaaac ctgagcattg caggccactg
     1261 ggtgagcgaa aagataggag cgaattcaat attccattca gaatgcagac tggagag
//
LOCUS       DAOQGJ010001434         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81890_length_1316_cov_3.444885, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001434 DAOQGJ010000000
VERSION     DAOQGJ010001434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81890_length_1316_cov_3.444885"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PK683_18500"
     CDS             complement(<1..595)
                     /locus_tag="PK683_18500"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNH10499.1"
                     /translation="MAGPAAKKNQGKKQGNEISLFQKLEPFYPFLLFFLSIFAGFSLF
                     SYDDGDYGTANNLFGRLGYLFSYICFYFFGRASYFISALSALSGLIIFQKRQADIRKL
                     AAAGLIVMTALSVFFYVFDTNIISLKDGGGKIGFMLSSFLEFLVGRSGRILVAAALLI
                     FGGLLILKESPKEINKEYWKVVFAKNREKIFKWLKPDD"
     gene            complement(685..>1316)
                     /locus_tag="PK683_18505"
     CDS             complement(685..>1316)
                     /locus_tag="PK683_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10500.1"
                     /translation="LGFLLFSRDEAEDMLPVLLLFSPAIISFLYNGAAFLMKTDYRFL
                     LKDHGTAEYSPSLLSSEKRFKGLIRFGGEFTLSEKPFSVTYTRAPKKNSSGGSYTLHF
                     FPVINSTNEETVGFINSFPFSSYKDLGNNPGKNLSGFLNACEYNVSEKYGKTPNAKKL
                     SEKIFCLKYFPEEEFNLQEFSEKAWPILWILSAVHFFICTAILLFWKEK"
BASE COUNT          400 a          262 c          292 g          362 t
ORIGIN      
        1 catcatccgg cttcagccat ttgaatattt tttcccggtt ttttgcaaat acaactttcc
       61 agtattcttt gttgatttcc ttgggagact ctttaagaat cagaagacct ccgaaaatca
      121 gaagtgcggc agcgacaagg attcttccgg aacggcctac caggaattcc aggaaggagg
      181 agagcataaa tccgattttt ccgccaccgt ctttcaggct gatgatattg gtatcaaata
      241 catagaaaaa tacactcagc gcagtcatca cgatcaggcc ggccgctgca agcttgcgaa
      301 tgtctgcctg tcttttctgg aaaatgatca gtccggacaa tgcagaaagt gcagagatga
      361 agtaggatgc ccgtccgaaa aaataaaagc agatatagga aaagagatat cccagtcttc
      421 cgaaaagatt attggctgtc ccatagtctc cgtcatcgta agaaaacaga gagaacccgg
      481 caaatatgga aaggaagaaa agcaggaatg gataaaacgg ctccagtttc tgaaaaagac
      541 tgatttcatt cccctgtttt tttccctgat ttttttttgc tgcaggtccc gccacgtata
      601 gttctccatt tctattttcg gaatgctttc tgaaaaaaaa ctaaaaagaa ttttgatcca
      661 gtttggaaat tagaccgata atcatcactt ttccttccaa aaaagaagaa ttgcagtgca
      721 gataaaaaaa tgtaccgcag aaagaatcca aagaatcggc catgcttttt ctgagaattc
      781 ctgcagatta aattcctctt ccgggaaata tttcaggcag aagatttttt cggagagttt
      841 ttttgcattc ggagtcttgc cgtatttttc ggaaacattg tattcacagg cattcaggaa
      901 accggagaga ttttttccgg gattgtttcc cagatctttg taagatgaga acgggaagga
      961 attgatgaat ccgacagtct cctcatttgt actgtttatg acaggaaaaa aatgcagagt
     1021 gtaggaaccg cctgaactgt tttttttcgg tgctcttgtg taggtgacag agaacggctt
     1081 ttcggaaagg gtaaattctc cgccgaaccg tatcagtcct ttaaaccgtt tttctgaact
     1141 gagaagagaa ggggaatatt cagctgtccc atggtctttc agaagaaatc tgtagtcagt
     1201 cttcatcaga aaggcagcgc cgttgtacag gaagctgatg attgcgggtg aaaagagaag
     1261 cagaacagga agcatatcct ctgcttcgtc tcttgaaaaa agaagaaagc ccagaa
//
LOCUS       DAOQGJ010001435         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81904_length_1316_cov_3.106265, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001435 DAOQGJ010000000
VERSION     DAOQGJ010001435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81904_length_1316_cov_3.106265"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..511)
                     /locus_tag="PK683_18510"
     CDS             complement(44..511)
                     /locus_tag="PK683_18510"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH10501.1"
                     /translation="MNTSPKNTDFSIRQASVSDLSELVPLFDAYRMFYGKDSDLKGAE
                     IFLQDRMKKNESVIFFAKEGKKTAGFVQLYPSFSSVSIGKIFVLNDLFVQERFRKMGA
                     GEMLIQKAVEYARSENAVRLVLSTGISNQSAQRLYEKLGWEKDVSFFYYSYSL"
     gene            728..>1316
                     /locus_tag="PK683_18515"
     CDS             728..>1316
                     /locus_tag="PK683_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNH10502.1"
                     /translation="MFKFILNLFRKKPASAEDMLQFPEGKRYYKETHNLRKNMMDDDA
                     IKIIYRLNKFGHKAFLVGGCIRDLLLGRKPKDFDIVTSATPNQIRNIFNNCRIIGKRF
                     KIVHVIFKGKVIEVSTFRSLPDHRLEPVNKKTDGKDKDYLLKKDNNYGTPKEDVARRD
                     FTVNSLYYDPRNESLIDFVGGYEDVKNKIVRVVGDP"
BASE COUNT          454 a          267 c          238 g          357 t
ORIGIN      
        1 tgaaaatctg aagactcata attttcactg aagcggaact ctttcacaga gaataactgt
       61 aataaaaaaa agagacatcc ttttcccatc cgagtttttc atagagtctc tgtgcagatt
      121 gattggatat tccggtagaa agaaccagtc tcaccgcatt ttcagaacgg gcatattcga
      181 ctgctttctg aatcagcatt tctcctgcac ccatttttct gaacctctcc tgcacaaata
      241 aatcattcag aacgaaaatc tttccaatgg acactgaaga aaagctggga tataattgca
      301 caaatcctgc ggtcttcttt ccttcctttg caaaaaaaat gactgattca ttttttttca
      361 ttctgtcttg cagaaagatt tctgcacctt tgagatcaga atcctttcca taaaacatcc
      421 tgtaggcatc aaataagggc actaattcgg aaagatctga aacggaggcc tggcggattg
      481 aaaaatcagt attttttggg gatgtattca tattcgttca gaacaaatta ctgaagcctg
      541 aattcaacag aaaaacttgt tcgacctgtt cgccggaaag caaaagaaaa aaaatcggta
      601 ctttacaaat tttatgcaat aaaaaaatcg tccaaactca tttgtattga cggaaaaaaa
      661 caggaagcgt gctcggtaca gggtttttag ttgaaacttc gtaccaatcc gaaaaacttg
      721 actaaaaatg tttaaattta tcctcaattt atttcgtaaa aagcctgctt cagctgaaga
      781 catgctccag tttccagaag ggaaaagata ttacaaagaa actcacaatc ttcgtaagaa
      841 tatgatggat gatgacgcca tcaagattat ctacagactc aataaattcg gtcacaaagc
      901 attcctcgta ggaggatgca ttagagatct tcttttaggc agaaaaccca aagacttcga
      961 catcgttacc agtgcaacac cgaaccagat ccgcaatatt ttcaacaact gcagaatcat
     1021 cggaaaaaga ttcaagattg tacatgtaat attcaaaggc aaggtcattg aagtttccac
     1081 tttccgttct cttccggatc acagactcga accggtcaat aaaaagactg acggaaaaga
     1141 caaagactat cttctgaaaa aagacaataa ctacgggact cccaaagagg atgtagccag
     1201 aagagatttt actgtcaatt cactgtacta tgaccccaga aatgaatcac tgattgattt
     1261 tgtcggcgga tacgaagatg tcaaaaacaa gatcgtcaga gttgtaggcg atccgg
//
LOCUS       DAOQGJ010001436         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81922_length_1316_cov_2.675654, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001436 DAOQGJ010000000
VERSION     DAOQGJ010001436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81922_length_1316_cov_2.675654"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PK683_18520"
     CDS             <1..353
                     /locus_tag="PK683_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10503.1"
                     /translation="ARNQDLTMFEYFLTDKTGHSQNWEHAKQVIATVEKFMDGVLSEL
                     NPETEHLIVTSDHGNLEDLSDKVHTSNPVPTFLYGKYSDSMKDKIKTLADIVPAIYSE
                     LNIPEKPVYVKQEG"
     gene            complement(684..842)
                     /locus_tag="PK683_18525"
     CDS             complement(684..842)
                     /locus_tag="PK683_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10504.1"
                     /translation="MQLDDTEAEFASISHVPYPVPLERGVLAPSEALKSEAELDDRAK
                     RSIAFCAV"
BASE COUNT          438 a          290 c          273 g          315 t
ORIGIN      
        1 tggcaagaaa ccaggatctg actatgtttg aatacttcct tacagacaag acaggtcatt
       61 ctcagaactg ggagcacgcg aagcaggtga ttgcaacagt ggaaaaattc atggatggag
      121 tcctgtccga gctgaatccg gaaacagaac atcttattgt cacatccgat catggaaacc
      181 tcgaagatct ttcagacaag gtgcatacat cgaatccggt acctacattt ctctacggaa
      241 aatattcaga ttcgatgaaa gacaaaatca agacccttgc ggatatcgtt cctgcaattt
      301 acagcgaact taatattccg gaaaaaccag tctatgtgaa acaggaaggc tgattaaacc
      361 ccggggaatc gggaatagcc ggagggaatt gatacgattt acaatgaaac tgctctgaca
      421 ggagcggaac ggcaaaagct cgagaaaagt ctctcaagaa gaacagactt tctgttctac
      481 gataaagaca ttccggaaag actgaaagga tgcgaatgca ggaaataaat tgaaagtgac
      541 tgtcaattca agacagaaca ggtattttta caagcgaaaa cgaaaaacaa ctcacaggag
      601 attataatga aaaaactatt gattccgttt attgcccttt tggcaattct gtccaatgcc
      661 ggatgcaccg agaaaaagaa agactaaaca gcacagaatg caatacttct tttggctctg
      721 tcatccagct cagcttcaga tttgagggcc tcacttggcg ccagaactcc tctttcaagc
      781 ggcactggat atgggacatg tgaaatgctg gcaaactccg cttcagtatc atccagttgc
      841 atatattact acagtgaaaa ccagtgttac attttaaaaa cagtcaatgg aggttatcac
      901 accatcaatt tttactcacg gagcacaaca accacagcag aacagcaaga caagacaacc
      961 ttcaataaat tagggtacac agactgctca tcttcagcag tatttggaac ttcatacggt
     1021 tgcgtaaaac catagtaaca taacaaggct ttgtatgaat aactaaatgg tcttccgagt
     1081 gattcgctaa ctcaagggaa tcactttttc agtcaagctc attaggcttc aggctgctaa
     1141 cgaggagttc ctgcttagat caagaaaagc ggtgtgcgat ggcaatgcca aggtgagatc
     1201 gaaaggcata tatctacaga agacatcatt caacccgcct ccactatcca ttcgccgaca
     1261 ttcctgtccc taaattccac accgaacaga taaatggggt cgtttttctg catccc
//
LOCUS       DAOQGJ010001437         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_81999_length_1315_cov_3.917460, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001437 DAOQGJ010000000
VERSION     DAOQGJ010001437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81999_length_1315_cov_3.917460"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1315
                     /gene="ftsH"
                     /locus_tag="PK683_18530"
     CDS             <1..>1315
                     /gene="ftsH"
                     /locus_tag="PK683_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNH10505.1"
                     /translation="EEAKTELVEIIDFLKDPKKFQAIGARIPKGVLLVGPPGTGKTLL
                     AKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKRNAPCIIFIDEIDAVGRL
                     RGAGMGGGHDEREQTLNQMLVEMDGFEANEGVIVIAATNRADVLDPALLRPGRFDRQV
                     VVDLPDVKGREEILKVHSKKVPLTSDISLNSIARGTPGFTGADLANLVNEAALIAARK
                     NQKRVTQIELEEARDKVLMGPERKSFFISDKEKEVIAYHEAGHTILATLLPFTEPVHK
                     VTIIPRGRALGLTQSLPSEEKHIHTKNYWMDQIVMAMGGFIAEEYKFGATSTGSSNDI
                     QHATNISRKMVCEWGMSEKLGTINYSGNHDSIFLGRDIGNQSKYYSEQFAALIDDEVR
                     KIVQTCLDKGRSLVRANAARLETIAKALLTQETVTAEELKSIIDNT"
BASE COUNT          411 a          266 c          338 g          300 t
ORIGIN      
        1 gtgaagaagc aaaaactgaa ctcgtggaaa tcattgattt tctgaaagat ccgaaaaaat
       61 tccaggcaat cggtgcaaga attcccaaag gagttctgct cgtcggtcct ccgggaacag
      121 gaaaaactct tctagcaaaa gcggtagcag gagaggctgg agtgcctttc ttcagcatat
      181 ccggatctga tttcgtagaa atgtttgtgg gtgtcggtgc ctctcgagtc agggacctgt
      241 ttgaacaggg caaaagaaat gctccctgca ttatcttcat tgatgagatt gatgcggtcg
      301 gaagactgag aggtgcagga atgggaggcg gccatgatga aagagagcag actctcaatc
      361 agatgctcgt cgagatggac ggctttgagg caaatgaggg agtgatcgtg attgctgcta
      421 caaacagagc agatgttctc gatcctgcac ttctgagacc tggacgtttt gacagacagg
      481 tggttgtaga tcttcctgat gtaaaaggga gagaagaaat tctgaaagtc cattccaaga
      541 aagttcccct gacaagcgac atatccttaa attcaattgc gagaggaacc cccggcttta
      601 caggtgcgga tcttgcgaat ctggtcaatg aggccgcact gattgcagct agaaaaaatc
      661 agaagagagt gactcagata gaacttgagg aagcaagaga caaggttctg atgggacctg
      721 agagaaagtc ctttttcatt tccgacaagg aaaaagaagt cattgcctat catgaagcag
      781 ggcacacgat ccttgcgact cttctacctt tcactgagcc tgttcacaag gtgacaatca
      841 ttccgagagg aagggcactc ggcctgactc agtctcttcc atcagaagaa aaacatatcc
      901 atacaaaaaa ctactggatg gatcagatag tcatggcaat gggcggtttt atcgcagagg
      961 aatacaaatt cggtgcgacc tccaccggtt caagcaatga tattcaacat gccacaaaca
     1021 tttccagaaa aatggtctgt gaatggggaa tgtcggaaaa actcggaacg attaattaca
     1081 gcggaaatca tgattccata ttcctgggca gggatatcgg aaatcagagc aaatactaca
     1141 gtgaacagtt tgcagcactg attgacgatg aagtacgtaa aattgtgcag acctgccttg
     1201 acaaaggaag aagtcttgtg cgtgcaaatg cagcaaggct tgagacaatt gcaaaggcgc
     1261 tcctgactca ggaaactgtg acagcagaag aattgaaaag catcattgac aatac
//
LOCUS       DAOQGJ010001438         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82056_length_1315_cov_2.334127, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001438 DAOQGJ010000000
VERSION     DAOQGJ010001438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82056_length_1315_cov_2.334127"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK683_18535"
     CDS             <1..322
                     /locus_tag="PK683_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10506.1"
                     /translation="RLDFQKGISLLEAVPHTGRTHQIRAVLFSLGFPLVGDKIYGKSE
                     EWFLRFIQSGKFEEDILHRQALHGAELKFIHPALNKEMHLIAEEPEDMKRIFLPDQTF
                     DVLP"
     gene            complement(252..1229)
                     /locus_tag="PK683_18540"
     CDS             complement(252..1229)
                     /locus_tag="PK683_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10507.1"
                     /translation="MVLHKKIVIVFFIIFALISAWYISGFVIYPNLQKRIEFVDEILK
                     GKSVQPYQYRVLKPFLGKKIGSLIPFNGHIKHFVGFSILNFFVFFGIYFLFFNYLKIF
                     YDEKISFIGVLILQILIPLSVSGFYMEGDFITLLFYTVGLMLLFKNKQYFLPVLMFLA
                     ALNREQFIFFSVWHFIFLYSFKRLDKKNLFILFLSVIMFWAGYHLPRLYFGIRENPYS
                     MEIHLKNNTDPFWLYLKIIPLWISEIAGFFILAVYAFRKSSLFFRLLLISLIPYTAAF
                     YVKANIWELAKYLPAMLVLIPMALGALTEERQTSGPAEKSASYPPAPLQ"
BASE COUNT          462 a          253 c          268 g          332 t
ORIGIN      
        1 aagactggat tttcagaaag gcatatccct actggaagcg gtcccgcata caggaagaac
       61 tcatcagatc cgtgcagttc tgttcagtct gggctttcct ctggtcggtg ataaaattta
      121 cggaaagtcg gaggaatggt ttctgcgttt catccagtcc ggaaaatttg aagaggatat
      181 tctgcaccga caggcactcc acggcgcaga actgaaattc attcatcctg cactgaacaa
      241 agaaatgcac cttattgcag aggagccgga ggatatgaag cggatttttc tgccggacca
      301 gacgtttgac gttcttccgt aagagcgccc agagccatcg gtataagaac cagcattgca
      361 ggcagatatt tagccagctc ccatatattt gcttttacat aaaatgccgc tgtataagga
      421 attagactga tgagcagaag tctgaaaaaa agagaacttt ttctgaaggc ataaacggcc
      481 agaataaaaa aacctgcgat ttcggaaatc cagagaggaa taatcttcag ataaagccag
      541 aatggatcgg tgttgttttt cagatgaatt tccatactgt atggattttc ccgaattcca
      601 aaataaagac gaggcagatg ataacctgcc cagaacataa tgacagacag aaacagaata
      661 aacagatttt ttttatccag tcttttgaag gaatacagaa aaatgaaatg ccatacggag
      721 aagaatataa actgttctct gttgagtgca gccaaaaaca tcagcaccgg caggaaatac
      781 tgcttatttt taaagagaag cataaggccg acagtgtaaa agagaagcgt gataaaatca
      841 ccctccatat aaaaaccgga gacggaaaga ggaatgagaa tctgaagaat taaaactcca
      901 atgaaggata ttttttcatc ataaaaaatc tttaaataat taaaaaacag aaaatagatt
      961 ccaaagaata caaaaaaatt aaggattgag aaaccgacaa aatgctttat atgtccgttg
     1021 aacgggatca gacttcctat ttttttgccc aggaaaggct tcaataccct gtactgatag
     1081 ggctgaacag actttccctt cagaatctca tccacaaact caattctttt ctgcaggttc
     1141 ggatatatga caaatccgct gatgtaccat gcagaaatca gggcaaaaat gatgaagaag
     1201 actattacaa tttttttatg aagtaccatt ggactccttc tgctgaatca gtatctttca
     1261 cagaaaaagc cgctgcagaa aatcagtaaa gaagaatttc cattggaatc tcctt
//
LOCUS       DAOQGJ010001439         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82156_length_1314_cov_2.361398, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001439 DAOQGJ010000000
VERSION     DAOQGJ010001439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82156_length_1314_cov_2.361398"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PK683_18545"
     CDS             <1..1220
                     /locus_tag="PK683_18545"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HNH10508.1"
                     /translation="KTGKPVYNAIVWQCRRTSDICADIKKQGLESEFRNKTGLVVDAY
                     FSGTKISWILDNVKGARDKAERGDLLFGTIDTWLLYNLTKGKSHATDHTNASRTLVYN
                     IKEKSWDKDLLNVLKIPSSLLPEVHPSRHIFGRTEGVKGLPDGIPIASLVGDQQGALF
                     GQLCTEVGEAKNTYGTGCFLLFNTGDECRISKQGLITTLACGPEGKTVYALEGSVFIG
                     GAVVQWLRDNMKFFKQSKDTQKIAQSIKKDDDVVFVPAFAGLGAPHWDMDARGAIYGL
                     TRDTTPEQITRAALKSIALQSYELVKAMEEDTGEALKVLKVDGGATFNDYLMQYQSDI
                     LGKKVIRPENVDTTVLGAAYLAGLETGFFKSVDELKKLTKKFKEYSPKMKSEIRDSEI
                     NKWHTAVIKTKTN"
BASE COUNT          397 a          278 c          332 g          307 t
ORIGIN      
        1 gaaagaccgg aaagcctgta tacaatgcga ttgtatggca gtgcagaaga acatccgata
       61 tctgtgcgga catcaagaag cagggacttg aaagcgaatt cagaaacaag acaggtcttg
      121 tcgtggatgc ttatttcagc ggaacaaaaa tcagctggat tctggacaat gtaaagggag
      181 caagagacaa ggcggaaaga ggtgaccttc ttttcggaac aatcgatacc tggcttcttt
      241 acaatctcac taagggcaag tctcatgcta cagatcacac aaatgcatcc cgtactcttg
      301 tttacaatat caaggagaag tcctgggaca aggatcttct gaatgtgctg aagatacctt
      361 cctctctcct tcccgaggtt catccttcca gacatatctt cggaagaacg gaaggagtca
      421 aggggcttcc tgacggaatc cctatagcat cccttgtggg agaccagcag ggggctcttt
      481 tcggacagct ctgcactgaa gtgggagaag ctaagaatac ttacggaact ggctgttttc
      541 ttctattcaa tacaggagac gaatgcagaa tttccaagca gggtctgatt accactcttg
      601 cctgcggacc ggaaggaaag actgtgtatg cactggaagg atctgttttc atcggaggtg
      661 cagttgtgca gtggctccgc gacaatatga aatttttcaa gcagtccaag gacactcaga
      721 aaattgctca gtccataaag aaagatgatg atgttgtctt tgttcccgcc tttgcaggac
      781 tcggagctcc tcactgggat atggatgcaa gaggagcgat ctacggtctg accagagaca
      841 caacccctga gcagatcaca agagcagcac tcaagtcgat tgcacttcag tcctatgaac
      901 tggtgaaggc gatggaagag gatacaggag aggcgctcaa ggttctgaag gtggacggcg
      961 gtgctacttt taatgattat ttaatgcagt atcagtcgga tattctcggg aaaaaagtca
     1021 tcagacctga aaatgtggac actactgtac ttggagctgc atatctggcg ggtctcgaaa
     1081 caggattttt caagtcggtg gatgaactga aaaaactgac aaagaaattc aaggaatact
     1141 ctcctaagat gaagtctgag atccgagaca gtgaaatcaa taaatggcac actgcggtaa
     1201 tcaagacaaa gaccaactga aaaaaccagg aagaaatcat taaaaaatat ttcttctgac
     1261 agcagacggg cgggagcgct cagtccgtct gcttttattc ctccggcaga caga
//
LOCUS       DAOQGJ010001440         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82226_length_1313_cov_3.103339, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001440 DAOQGJ010000000
VERSION     DAOQGJ010001440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82226_length_1313_cov_3.103339"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK683_18550"
     CDS             <1..322
                     /locus_tag="PK683_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10509.1"
                     /translation="ITSDSASAYTQSRADSDRQTWKYTVRFDSQRPSNMTSLTETNTY
                     SYPVPAPGSGYFQNQYTRNYFWIYDDRGRIVRDSTYIYSYDSIGLLSYSKGTDNSIVE
                     FFSR"
     gene            377..>1313
                     /locus_tag="PK683_18555"
     CDS             377..>1313
                     /locus_tag="PK683_18555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH10510.1"
                     /translation="MNFHWKNYRILIPSLLIVGLLLFGLGRWIYYANKNDIHIAIVGP
                     MTGTSSANGLSYVQGVKLFTEKINQSGGIDGKHIVLNIYDDQNKADLAKAKAEELVKK
                     NRVLAVIGHNSSDCSIAAGEIYKTAEIPAITPASTNVKVTVGNEWYFRTIFNDNSQGR
                     FLANYVKKILKYSEVKIVHEELRYGKYLTEVFEATAKEIGLKITYKKEFSTVSPTKEK
                     DLENIVKELKAKNDNQLIFIPMHVPEGAVFVRLLKDHGLNNPVIAPDAFASAGFIEEL
                     KKQKREQLNPGFYSNEMLVTTPLIFDNANQKAQQFR"
BASE COUNT          420 a          273 c          287 g          333 t
ORIGIN      
        1 aatcacatcc gatagcgcct ctgcttacac tcagtcacgg gcagattcag acagacagac
       61 ctggaaatat actgtgcgct ttgacagtca gagaccctca aatatgacca gtctgacaga
      121 aacgaacacc tacagttatc cggttcctgc accgggttca ggttatttcc agaaccaata
      181 caccaggaac tatttctgga tttatgacga caggggcagg attgtaagag attccacata
      241 tatatattca tacgattcca tcggcctgct ctcctactca aaaggcacgg acaattcaat
      301 tgtagagttt ttcagcagat aattactgcg atttttgctt gaacggtgta ctctatcctt
      361 gcaactggta agttagatga actttcactg gaaaaactac cgtattctga ttccctccct
      421 gcttattgtc ggactgctgc ttttcggttt aggaagatgg atttactacg caaacaaaaa
      481 tgatattcat attgcaattg taggtcctat gaccggaaca agttctgcga acggactgtc
      541 ttatgtacag ggggtcaagc tctttactga gaaaatcaat cagtctggcg ggattgacgg
      601 aaaacatatt gttctgaata tttatgatga ccagaacaaa gccgatctgg caaaggccaa
      661 ggcggaagaa cttgtaaaga aaaacagggt tctggcagtc atcgggcaca acagcagcga
      721 ctgctccatt gccgcaggcg aaatctacaa gactgcagaa attccggcaa ttactccggc
      781 atcgaccaac gtaaaagtga cggtaggaaa tgagtggtat ttcagaacaa ttttcaatga
      841 taattctcag ggacgttttc ttgcaaatta tgtaaagaaa attctcaaat actcggaagt
      901 caaaatcgtg catgaagagc tgagatacgg aaaatatctg actgaagttt tcgaggctac
      961 tgcaaaagag atcggactga agatcactta caagaaggaa ttcagcactg tcagtcctac
     1021 aaaggaaaaa gatctggaaa acattgtaaa ggaactgaaa gccaagaacg acaatcagct
     1081 tattttcatt ccaatgcatg ttcctgaggg ggcagtgttt gtcagacttc tgaaggacca
     1141 tggactgaac aatccagtga ttgcacctga tgcatttgca tcggcaggtt ttatagaaga
     1201 gctgaaaaaa cagaaaagag aacagctcaa tccaggattc tattcaaatg aaatgctcgt
     1261 gactactcct ctgattttcg acaatgcaaa tcagaaagca cagcagtttc ggg
//
LOCUS       DAOQGJ010001441         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82351_length_1312_cov_2.787589, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001441 DAOQGJ010000000
VERSION     DAOQGJ010001441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82351_length_1312_cov_2.787589"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..659)
                     /locus_tag="PK683_18560"
     CDS             complement(225..659)
                     /locus_tag="PK683_18560"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH10511.1"
                     /translation="MRRIIADDSAVFSFSPEKVYSAVSDMSTYEKWWGKKVKVKVLEL
                     SPQGVGSKVEVHAVGGWFRCEIISLKPFEEAGILYYDGVQKGKGVWKIEKLGENETAL
                     SYSIDLEPNGFVPRFLSNFMNFSKLHSQAMKDLFSGLENFLA"
     gene            821..1129
                     /locus_tag="PK683_18565"
     CDS             821..1129
                     /locus_tag="PK683_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10512.1"
                     /translation="MRHSEYLKAQLVILILSMSAGCNYKLDCTNPDDNINLLKSFDHG
                     CPGSAEYKKAKKDCIQSNLAAYAMLCNKPNPDSAVQMFCPQYLFSALGGCPDSNGGGF
                     "
BASE COUNT          395 a          279 c          226 g          412 t
ORIGIN      
        1 ggtcaggttc cgcagatgca aaaaatttgt acctttcaga aaggttttca aacctgtcct
       61 tcttaagcag attcataaaa tattctattt tatcttcttt tattttaaag tcatatttca
      121 cagtttttct cactcagaat attgtttcaa taataaagtt ttagtcaatt cttcattgat
      181 ccaattctaa tgtaatctga aatccggttt tttttactgc tgctctaggc aagaaaattt
      241 tccagaccgg aaaataaatc cttcattgcc tgcgaatgca gttttgaaaa attcataaaa
      301 ttggacagaa atcttggaac aaatccattc ggctccaggt cgatagaata agaaagtgca
      361 gtttcatttt ctcccaattt ctctattttc cagacccctt ttcccttctg aactccgtca
      421 taataaagaa tacctgcttc ttcaaaaggt ttcagagaaa tgatttcgca gcggaaccag
      481 cctccgactg catgtacttc cacttttgaa ccgacccctt gagggctgag ttccagaact
      541 tttactttta ccttctttcc ccaccatttt tcataggtgc tcatatcaga aacagcactg
      601 taaacttttt ccgggctgaa actgaaaaca gccgaatcat ctgcaataat cctgcgcata
      661 ttccttttcc taaaagaaat tgattataga gagtaaatat taatctgtca gattcagtca
      721 agtcactctt ttgcaaggct ggaatatttc agagacaatg tgcagtgctt ctgcggtttt
      781 tagacagagc gacttcacgg gaaattccga tcatcgtaat atgagacatt ctgaatacct
      841 gaaagcgcag ctggtcattt taattctgtc aatgagtgcc ggatgcaatt ataaactgga
      901 ttgcacaaat cctgacgaca acattaatct tttgaaaagt tttgatcacg gctgccccgg
      961 ttcagcagaa tataaaaaag caaagaagga ctgcattcag agcaatctcg ccgcctatgc
     1021 aatgctctgc aataagccga atccagactc agcagtccag atgttttgtc ctcaatatct
     1081 gttttctgct ctgggaggct gtcctgattc aaatggcgga ggcttttaga agactctcat
     1141 acagaatctc ttcctgtcga ttgagcagta aattaaaagg aacaattcct cttcaaaaaa
     1201 tgattgatct aattttacct tatctgcata tagtcggggg tattgccgga caataaatcg
     1261 gttcaactct cataaaggaa aaaaaatgaa ttcagaactt ctcaaggctg tc
//
LOCUS       DAOQGJ010001442         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82413_length_1312_cov_1.638027, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001442 DAOQGJ010000000
VERSION     DAOQGJ010001442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82413_length_1312_cov_1.638027"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PK683_18570"
     CDS             complement(<1..324)
                     /locus_tag="PK683_18570"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNH10513.1"
                     /translation="MKGFKSTREFIETLKTENELLEIHEEVDPVEEIAEIQRRVVRRK
                     GPAVLFHKVKNSKFPAAGNLYGSEKRIELAFGKKPKEIIAKLAHAAETILPPSFSKLW
                     DARELG"
     gene            complement(328..>1312)
                     /locus_tag="PK683_18575"
     CDS             complement(328..>1312)
                     /locus_tag="PK683_18575"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNH10514.1"
                     /translation="AGFSITNDLGFLTKNTYCLYTISALIILGGIGYPVMIFIEKSLL
                     ELLYKVVSKLEVLGETYLMRQAIKGEEPSKMYFMFTKLSLWTEYRIESYNKSLFGESN
                     KVQTNIIIYGTVALLFLGTSFVMALEFGNPETLGALNFSEKLANSVLMSASSRTAGFN
                     TFPVGKILDPTVILICCLMFIGGGPQGTAGGIKITTFAILVKYLGNVISSSSNVAIFG
                     NTISKRSVAMSIRLYFLATSTLAAVIFVLTVLHRDQGRIPQIIFEVISAFGTVGFSLG
                     ITPVLGDAEKLIYCLLMYIGRIGIFTVLIAMTGNPVTSPIGQDDGVKIQVG"
BASE COUNT          359 a          285 c          268 g          400 t
ORIGIN      
        1 accaagctcc cttgcatccc agagtttaga aaaactgggc gggagaattg tttcagcggc
       61 gtgagcaagt tttgcaatga tttccttcgg cttttttccg aatgcaagtt ctatcctttt
      121 ctccgacccg taaaggtttc cggctgcagg aaattttgag ttttttactt tgtgaaaaag
      181 aactgcagga cctttccttc tgacaacacg cctttgaatt tctgcgattt cttccaccgg
      241 atctacttct tcgtgaattt cgagaagttc attttctgtt ttcagagttt caataaattc
      301 tctggtagac ttgaatcctt tcatttttta cccaacctga attttaactc cgtcatcctg
      361 accgatcgga ctagttaccg gatttcctgt cattgcgatg agtactgtaa atattccaat
      421 tcttccgata tacattaaga gacagtatat cagtttttct gcatccccta gaacgggcgt
      481 aatgccgagt gaaaacccca ctgttccaaa tgcagagatc acttcgaaaa tgatctgcgg
      541 aattcttccc tgatcccgat gaagaaccgt cagaacaaaa atgactgcgg caagagtcga
      601 tgtggccaga aaataaagcc ttatgctcat tgcaacggat ctttttgaga ttgtgtttcc
      661 aaagattgcc acattgctcg acgagctgat cacgtttcct aaatatttaa caagtattgc
      721 aaatgtagtg atttttatcc cgcctgcagt tccctgaggg cctcccccga taaacatcag
      781 acagcagatg agtatgacag tgggatcgag aatttttcct acagggaagg tattgaaacc
      841 ggctgttctc gaggaggcgg acataagaac ggaattggcc agtttttctg aaaaattgag
      901 tgctcccagg gtttcaggat ttccgaattc cagtgccatc acgaaacttg ttccgaggaa
      961 aagaagtgca actgttccat agataatgat gtttgtctgt actttgttcg actctccaaa
     1021 cagagattta ttgtacgatt ctatcctgta ttctgtccat agagacagtt tagtgaacat
     1081 aaaatacatc ttagagggct cttccccttt gattgcctgt ctcatcagat atgtttcacc
     1141 cagaacttcc agcttcgata caactttata gagaagttcc aaaagagatt tttctataaa
     1201 aatcattacc gggtatccga tgcctcccaa tatgatgagt gcggaaatgg tgtacaggca
     1261 gtaggtattt ttggtcagaa agcccagatc attggtaatt gaaaaaccgg ca
//
LOCUS       DAOQGJ010001443         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82467_length_1311_cov_2.849522, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001443 DAOQGJ010000000
VERSION     DAOQGJ010001443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82467_length_1311_cov_2.849522"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..1275
                     /locus_tag="PK683_18580"
     CDS             412..1275
                     /locus_tag="PK683_18580"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH10515.1"
                     /translation="MKNVVITGCSSGVGLGLVKKFASRDGYKVHAAVRKITEELKKEL
                     LQNSEDRVVLHRMDVCDTEAVRQTVDSIIKNDGKIDILVNNAGYSQFGCVEMLSMDEV
                     QAQFNTNLYGVIRTSQAVLPSMRKNKWGRIINVSSIGGIYGQPFNDIYCASKFALEGL
                     SMSMASLYRQFGIRVVLIEPGAIRTSFVQNAVRPDISSLDSEFLPYLNSLLKTYESMF
                     QDASRSQTSEEVAEYIVAAAEQDDPDFRIQTNPATKGIYEMILKDPSGRTETEMSERV
                     FFSNLPAKKTD"
BASE COUNT          391 a          249 c          302 g          369 t
ORIGIN      
        1 atctggcaca tcaaatctat cttgactcat ataaccaata ctaattatac taaaatttgt
       61 acaagcaggt tttgggacgt gctcttagat atctattgac tgcgctctgc ctgacggcta
      121 ttagaaatct aggaagagtt tggagtccct cgaaagtgaa agctgctgca aaccccatcg
      181 gaataaccgt ttttgctcac tgcagtgtcg gccagaattg acttttaacc gatgttttat
      241 tgatccgatt ataatgtaat tagaaatttg tgtattgata tgcaataatg ggatatccta
      301 tatgtatgat gtaattttca caaagtatct gggagatcca gcattaaaga aataattttt
      361 ttgctttgaa attccgggga atcggaaaat ctgcaaatga ggagagaaag aatgaagaat
      421 gttgtgatta ccggatgttc ctccggagtg ggtctcggtc tggtcaaaaa atttgcttcc
      481 agggacggtt acaaggttca tgctgcagtg agaaaaatta ctgaggaact gaagaaagag
      541 cttctgcaga attctgaaga cagggttgtt ctgcacagga tggatgtatg cgacactgaa
      601 gctgtaagac agacagttga ttccatcatt aaaaatgacg gaaaaataga tatactggta
      661 aacaatgccg gttattccca gttcggatgc gtcgagatgc tttcgatgga tgaggtgcag
      721 gctcagttca atacaaatct ttacggtgtc atcagaactt cccaggccgt gctcccttcc
      781 atgagaaaaa ataaatgggg aaggatcatc aatgtcagtt ccatcggcgg aatatacgga
      841 cagccgttca acgatatcta ctgtgcaagc aagttcgcac tcgaaggtct ttcgatgagc
      901 atggcatctc tttacagaca gttcggaatc agagttgtgc tcattgagcc gggggcaatt
      961 cgtacctcat ttgttcagaa tgctgtgcgt ccggatatat cgtctctgga ttcggaattt
     1021 cttccttatc tgaattctct tctgaaaact tatgaatcaa tgtttcagga tgcttccagg
     1081 tcgcagactt ccgaagaagt tgcagaatac attgtcgcag ctgcagaaca ggatgatcct
     1141 gatttccgta ttcagacgaa ccctgccact aagggaattt atgaaatgat attgaaagat
     1201 ccatccggca gaacagagac tgaaatgagt gagagagtat ttttctcgaa tctcccggca
     1261 aaaaaaactg actgatacag tgaacagatc gggacagatc tgtctgcatc a
//
LOCUS       DAOQGJ010001444         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82474_length_1311_cov_2.726911, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001444 DAOQGJ010000000
VERSION     DAOQGJ010001444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82474_length_1311_cov_2.726911"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /gene="lexA"
                     /locus_tag="PK683_18585"
     CDS             <1..501
                     /gene="lexA"
                     /locus_tag="PK683_18585"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNH10516.1"
                     /translation="ITAKGAYDHLKALEKKGYIKTSKNQSRGLELLRQKSEDKIPVHV
                     MSVPLVGRVAAGLPILAEENIEDYIPVSDELAKKGTVYALRVVGDSMIGAGINDGDIA
                     IIQKKETAKNGELVVAMIENEATLKAYYKETDHVRLEARNKAYKPIKAKKVEILGKLI
                     GLYRVY"
     gene            612..866
                     /locus_tag="PK683_18590"
     CDS             612..866
                     /locus_tag="PK683_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10517.1"
                     /translation="MSVLSFHCSNSKTEIADKDMESVLEKFAMYRFHQSLEMEDPDMR
                     PSDRKILQEICSQYRYSCPKVLEKIKSKYPILSKRLENEK"
     gene            856..>1311
                     /locus_tag="PK683_18595"
     CDS             856..>1311
                     /locus_tag="PK683_18595"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNH10518.1"
                     /translation="MKNKERLFWLFSTMLLAAYSFFPFEKAKAISLEGEKFLQVFHEI
                     SALIDSDFVETSEDKNLYQGAIKGMISTLGDPHTRFMDEEEFKQLQEETRGSFGGVGM
                     EVAPTEGGILVVAPIDDTPAQRAGVQPQDKIIEINGQSTEKMSIQDAIKI"
BASE COUNT          421 a          247 c          303 g          340 t
ORIGIN      
        1 attacggcaa aaggtgcata tgatcatctg aaggcacttg aaaaaaaggg ttatatcaaa
       61 acttccaaaa atcagtccag gggactcgaa ctcctccggc agaaatctga agacaagatt
      121 cctgtgcatg tgatgagtgt tccccttgtg ggaagggttg ctgcaggtct tcctatactg
      181 gctgaagaaa atattgaaga ctatattcct gtttcggacg aacttgcaaa gaaaggaaca
      241 gtctatgccc tccgtgtagt aggggattcc atgattggtg caggcatcaa tgacggagat
      301 atcgccatca ttcagaaaaa ggaaactgcg aaaaacggag agcttgttgt ggcaatgatt
      361 gaaaatgaag ctacattgaa agcttactac aaagagacag accatgtccg actggaagcg
      421 agaaacaaag catataaacc cattaaggca aaaaaggtgg aaattttagg aaaactcata
      481 ggactctata gagtttacta attgacaaac ctcttaccgg taaagatagt ccttttagaa
      541 tgtttttacc gaaaagaggc ctgcctgata atcttttgaa caggatgcgc aggtttttca
      601 gtttactgct tttgtctgtt ctttcgtttc actgttccaa cagcaagact gaaattgcag
      661 acaaggatat ggaaagcgta ttggaaaaat ttgcaatgta ccgtttccat cagtcattgg
      721 agatggaaga tcctgatatg cgcccatccg accgtaaaat actccaggaa atctgctccc
      781 agtacaggta ttcctgcccc aaagtactgg aaaaaattaa atccaaatat ccaattctgt
      841 ctaagagact agagaatgaa aaataaagaa agactattct ggctgttcag tacaatgctg
      901 cttgcagcgt attccttttt cccctttgaa aaagcaaaag caatttcgct ggagggagaa
      961 aaattcctgc aggtatttca tgaaatttct gcactgattg attccgattt cgtggaaact
     1021 tcggaggaca agaatttata tcaaggtgca attaaaggaa tgatttccac tcttggagat
     1081 cctcataccc gatttatgga tgaggaggaa ttcaagcagc ttcaggaaga aacacgcgga
     1141 agtttcgggg gagtggggat ggaagtggct cctacggaag gcgggattct tgtcgttgct
     1201 ccaattgatg acactcctgc gcagagagca ggtgttcagc ctcaggataa aatcattgag
     1261 attaacggtc agagcactga aaaaatgtcg attcaggatg ctatcaagat t
//
LOCUS       DAOQGJ010001445         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82477_length_1311_cov_2.679936, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001445 DAOQGJ010000000
VERSION     DAOQGJ010001445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82477_length_1311_cov_2.679936"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..643)
                     /gene="wrbA"
                     /locus_tag="PK683_18600"
     CDS             complement(38..643)
                     /gene="wrbA"
                     /locus_tag="PK683_18600"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNH10519.1"
                     /translation="MKLLVLFYSTYGHLYQMALAAAEGAKEAGAEVTVKRVPETLSSE
                     IIAMQGATEAQKAFAHIPVATVDELPGYDAIIVGAPTRFGNVCGQMRAFWDATGGLWA
                     KGALIGKVGSVFTGSATQHGGQETTITSMQVTLLHHGMVIAGLPYSFAGQMRIDEITG
                     CSPYGASTITGGKGERMPSANELDGVKFQGKHVASIAKKLK"
     gene            833..>1311
                     /locus_tag="PK683_18605"
     CDS             833..>1311
                     /locus_tag="PK683_18605"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10520.1"
                     /translation="MRDIFRNMTNLVLNEMEREDMFILIGPRQIGKTTMLQNLERTLK
                     NRGEKTLFFNLDFESDRKFFDSQDILLSKIELEIGKRKAYIFADEIQRKENAGLFLKG
                     IYDKKFPYKWILSGSGSLELKEKIKESLAGRKRMYELNPVHFLEFFHYKTDYKYLSK"
BASE COUNT          372 a          283 c          267 g          389 t
ORIGIN      
        1 aactcgatca gtaatcaaga ggtacactca gggcatctta tttcagtttt tttgctatcg
       61 atgcgacgtg tttcccctga aatttcactc cgtccagttc gttcgcactc ggcattcttt
      121 ctccctttcc tccggtgatg gtcgacgcac cgtagggtga acatcctgtg atttcatcta
      181 tcctcatctg tcctgcaaaa gaatagggga gcccggcaat gaccattcca tgatgcagaa
      241 gagtgacctg catagatgtg attgtagttt cctgtccgcc gtgctgagta gcagatcccg
      301 taaaaacgct ccccactttc ccgatcagtg cacctttcgc ccagagacct cctgtagcgt
      361 cccagaatgc tctcatctga ccgcagacat ttccgaatct tgtaggagct cctacaatga
      421 tcgcatcata accagggagt tcatctacag tggccaccgg aatatgtgca aacgctttct
      481 gagcctcagt ggctccctgc atcgcaatga tttcactgga aagagtttca ggaactcttt
      541 tcactgtcac ttctgccccc gcttcttttg cgccctcggc cgcagccagt gccatctgat
      601 acagatgccc atatgtagaa taaaacaaaa ccagtaattt caaaatcagt tctccttctg
      661 tgtttataac agtttcaata tagtttgaca tcaaactaat ttctcaaatt cccgcaagca
      721 tattttacta tttaggtgtg attcttgctt tctctataaa gaaagaatat attctcttta
      781 acccgattaa aatatatatt gactttatta aatatcgggt taatttgaca gtatgagaga
      841 catttttcga aatatgacga atctggttct gaatgaaatg gagcgggaag atatgtttat
      901 tctgatcggg ccgagacaga tcggaaagac gactatgctc cagaatctgg aaagaactct
      961 caaaaacaga ggagagaaaa ctcttttttt taaccttgat tttgaatcgg acaggaaatt
     1021 ttttgactct caggatattc ttctgtccaa gattgaactg gaaatcggga aaaggaaagc
     1081 ctatatattt gcggatgaga ttcagagaaa ggaaaatgca ggtctttttc tgaagggaat
     1141 ttatgataaa aagtttcctt acaagtggat tctgtccgga tcgggcagtc tggaactgaa
     1201 ggaaaagatc aaggagtccc tggccggaag aaaaagaatg tatgagctga atcctgtaca
     1261 ttttttagaa ttttttcatt ataagacaga ttataaatat ctctctaagg a
//
LOCUS       DAOQGJ010001446         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82673_length_1309_cov_3.346890, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001446 DAOQGJ010000000
VERSION     DAOQGJ010001446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82673_length_1309_cov_3.346890"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1309)
                     /locus_tag="PK683_18610"
     CDS             complement(<1..>1309)
                     /locus_tag="PK683_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10521.1"
                     /translation="IFFFSVALTAREKNYKFYLSFVLSLAMQVISSGVYEMVWHLHFI
                     YPVMILCFNYKLILDSIRNRWVTVVWLFLLFSYVAVYFISNSAFIKSAGNSPNLEKVN
                     LLFGIQVSKRLYEVMLYWIDGFNGSSFITFKYLLKNMLDLNNYREIETVFLLSMLLVL
                     ASLILIKMDLPAIEWKYLILGFIFSVLLIFSGVILPSFSYGIGYGNRLIHFSSYGAIL
                     FLVFFILMLRRFGNVYRFIIILLSFAVVFSSSLTLKSAAQGFSRVHSRHFAYWRSLLT
                     EIPSLKKNSVLILENHPFSGTYEFSDTEYLRNFLHKKCIYYFNTPFNEIVDEGKSIKI
                     YGTMRNPMEQCENTDPETESLFYENIGLFWSSNWDKRFSVISKDRVIHLKWINEEMKI
                     EIDPSRSNMSLIESNNLDNYAQNLLNLKIYQERLAKGERMIDY"
BASE COUNT          475 a          256 c          202 g          376 t
ORIGIN      
        1 taataatcta tcattcgttc acccttggcc agtctttcct ggtagatctt cagattcaat
       61 aaattctgcg cataattatc cagattattg gactcaatta aactcatatt tgaccgagac
      121 ggatctattt ctattttcat ctcttcatta atccatttca gatgaatcac tctgtccttg
      181 gaaatcacag aaaatcgttt atcccaattt gaactccaaa acaaaccaat gttttcataa
      241 aataaagatt ctgtttcagg atcagtgttc tcgcactgtt ccattgggtt tctcattgtt
      301 ccatatattt ttatactttt tccttcatct actatttcat taaacggagt attgaaataa
      361 tagatgcatt tcttatgcag gaaatttctt aagtactcag tatcgctgaa ttcatatgtt
      421 ccagaaaaag gatgattctc taaaattaaa acagaatttt ttttcagact gggaatttcg
      481 gtcagaagac ttctccaata agcaaaatgc ctgctgtgaa ctctgctgaa tccctgtgca
      541 gccgacttca gagttaaact cgaagaaaac acaacagcaa atgataagag tataattata
      601 aatctatata catttccaaa ccgcctcagc attaatataa aaaataccaa aaacagaata
      661 gctccgtacg aagaaaaatg aatcaatcgg ttcccgtagc ctataccata ggaaaaagac
      721 ggaagaatta caccggaaaa tatgagtaag acagaaaaaa taaaacctaa gattaagtac
      781 ttccattcta ttgcaggaag atccatcttg atcagaatca gtgaagctag aaccagcaac
      841 attgacaaga gaaacacagt ctcgatttcc ctgtaattgt tcaagtccag catattcttc
      901 agcaaatact taaaagtgat aaaactggaa ccattgaacc catctatcca atagagcata
      961 acctcgtaca gtcttttact gacttgaata ccgaagagaa gatttacttt ttctaaattc
     1021 ggagaattac ccgcagattt gataaaagct gaattgctga taaagtagac agcaacatat
     1081 gaaaagagca aaaaaagcca tacaacagtc acccatctgt ttctgatcga atcgagaatg
     1141 agcttataat taaagcagag aatcattaca ggatatataa aatggaggtg ccagaccatt
     1201 tcatatacac cgctggagat tacctgcatt gcgagtgaga gaacaaaact cagataaaac
     1261 ttataatttt tctcccgcgc agtcaatgca acagaaaaaa agaaaattg
//
LOCUS       DAOQGJ010001447         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82686_length_1309_cov_3.066986, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001447 DAOQGJ010000000
VERSION     DAOQGJ010001447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82686_length_1309_cov_3.066986"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK683_18615"
     CDS             <1..531
                     /locus_tag="PK683_18615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH10522.1"
                     /translation="LFNSVTQPTIVLLAIPFGLIGVIWSFYLHGRPFSFLGLIGVIGL
                     SGIVVNNSIMMVEFINKIVSDRMEIQERFSSAALIDDIIDGAVRRLRPIVITTATTVF
                     GLLPTAYGIGGADPFIEPMVLALAYGIIVSTFITLILIPAFYMANLDGYFFGKRVLAG
                     VKNLSSSVKSRLKKEK"
     gene            528..>1309
                     /locus_tag="PK683_18620"
     CDS             528..>1309
                     /locus_tag="PK683_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10523.1"
                     /translation="MTKTESIFREKEIKSLRLLSYGRIAMMLLMGVSTFSVTNVLQEK
                     VFSIALIVFSTLLNILNLALLRRKKYITFAGYVGVFMDSVMLTLLPFIWRSSAGGSSV
                     PYAYFLKTSLSMISVIIIIINGLALRPAYPLILSVYAVILHIYFFIMTMKDTRTVFSE
                     NWMEVVLGPGVNPSFYATYIQIVFASGIVMALICHIARKTAYEAVRLETVNAQLGRYF
                     SPNVASEISESESILSSPGGKIQKVTVLFTDIRGFTSLSEKL"
BASE COUNT          333 a          284 c          302 g          390 t
ORIGIN      
        1 cttttcaatt cggtgactca gccgacgatt gttcttctgg caattccttt cggactcatc
       61 ggagtcatct ggtcttttta tctgcacgga agaccctttt cctttctcgg tctcatcgga
      121 gtcataggac tctcggggat cgtagtgaac aattcgatta tgatggtgga attcatcaac
      181 aagattgttt ctgacaggat ggaaattcaa gagagattca gttcagcagc actgatcgac
      241 gatatcattg acggtgcagt cagaaggctt cgtccgatag tgatcacgac tgcaactaca
      301 gtgttcggac ttctccctac agcgtacgga atcgggggag cggacccttt cattgaaccg
      361 atggtgcttg cactcgcata cgggatcatc gtatccactt tcattacact gatactgata
      421 cctgcatttt atatggcgaa tctggacgga tattttttcg gtaaaagagt tctggcaggc
      481 gtgaaaaatc tctcctcttc cgtaaagtcc agactgaaaa aagagaaatg accaagacag
      541 aaagcatatt ccgggaaaag gagatcaaaa gcctgcgtct cctttcctac ggaagaatcg
      601 caatgatgct tctgatgggg gtatccactt tttcagtaac caatgttctt caggaaaaag
      661 tattttccat tgcactgata gtgttcagca ctctgctgaa tattctgaac ctggctcttc
      721 tgcgcaggaa aaaatatata acctttgccg gatatgtcgg agtcttcatg gattcggtca
      781 tgctgacatt gcttccgttc atctggcgtt catctgccgg aggaagttcc gttccctatg
      841 cttatttttt gaagacaagc ctttcgatga taagtgtaat tatcatcatc ataaacgggc
      901 ttgctctgcg tcccgcgtat cctctgattc tttcagtcta tgcggtcatc cttcatattt
      961 attttttcat tatgacaatg aaggatacga gaaccgtatt cagcgagaac tggatggaag
     1021 tcgttctggg tccgggtgtc aatccaagtt tttatgccac ttacattcag attgtatttg
     1081 cgtcgggaat tgtgatggca ctgatctgtc atatagcaag aaagactgcc tatgaggcag
     1141 tccgtctgga aactgtaaac gctcagctgg ggaggtattt ttcaccgaat gtggccagtg
     1201 agatttccga atcggaaagc attctttcca gtccaggcgg aaaaattcag aaggtaactg
     1261 ttctgtttac ggatatcaga ggatttacct ccctcagtga aaagctgag
//
LOCUS       DAOQGJ010001448         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82797_length_1308_cov_3.388667, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001448 DAOQGJ010000000
VERSION     DAOQGJ010001448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82797_length_1308_cov_3.388667"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK683_18625"
     CDS             complement(<1..193)
                     /locus_tag="PK683_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptosyltransferase"
                     /protein_id="HNH10524.1"
                     /translation="MNLLVIRFSAMGDVALLTPALKSILAEYPNVQITFLTRGNFAPF
                     FFNIPNLNVIGLNFKSRRYK"
     gene            complement(196..795)
                     /locus_tag="PK683_18630"
     CDS             complement(196..795)
                     /locus_tag="PK683_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HNH10525.1"
                     /translation="MIPNSDQLVSVMIQSVIDWHKELKIHSNPYKEGSLEFLIYKKNQ
                     IDTVQWHVEDEIRRPDLPDSELVKFKRQIDALNQERTDTVELLDDLFYAMYKDIPKKE
                     KSRMNSETPAWLIDRMSILELKIYHMKEQTERKDASAEHIEKCSAKLAILISQRSDLK
                     TCLSELMDDLASGDKFMKVYRQMKMYNDQNLNPSLYQKK"
     gene            complement(832..>1308)
                     /locus_tag="PK683_18635"
     CDS             complement(832..>1308)
                     /locus_tag="PK683_18635"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH10526.1"
                     /translation="INSEKLDRIRLVFKGTDSVVNPDVYLPYRDELQPGAERFELSTA
                     NYSDWVYFRHTLMMLSEIGFDRVRMRIHELSDFLSDLLKKKGYSLASEKFSEKTGIIA
                     CSKKNLDSGKTAAGLKEKKIICADRLGRVRFSPHIYNSFEDLEKTVSILESTAVFR"
BASE COUNT          347 a          271 c          250 g          440 t
ORIGIN      
        1 ccttgtatct tctgcttttg aaattcagac cgattacatt cagattggga atattgaaaa
       61 agaaaggggc aaaatttcct ctggtcagaa aagtgatctg cacattcgga tattctgcaa
      121 gaatgctttt gagtgcaggt gtaagcagtg caacatcacc cattgctgaa aaacggatta
      181 caagaagatt cattcttatt tcttctgata cagtgaggga ttcagattct gatcattgta
      241 cattttcatc tgacggtata ctttcataaa cttatcgccc gatgcaagat catccatcag
      301 ttcagagaga caggtcttca gatcagatct ctgggaaatt agaattgcaa gctttgcact
      361 gcatttttca atatgttctg cagaagcatc ttttctttct gtctgttcct tcatatggta
      421 tatcttgagt tcgagaatgc tcatcctgtc tataagccag gcaggagtct ccgaattcat
      481 tctggacttt tccttcttag gaatatcttt gtacattgca taaaaaagat catccagaag
      541 ttcaactgta tcagtccttt cctggttcaa tgcgtctatc tgtcttttga atttcacaag
      601 ttcgctgtct ggaagatccg gccttctgat ttcgtcttct acatgccact gcactgtatc
      661 tatctgattc tttttgtaga tcagaaattc tagacttcct tctttgtaag gattggagtg
      721 aattttcagt tccttgtgcc agtcaattac ggactgaatc attacggaaa ccagttggtc
      781 ggaatttgga atcatatctt ttatcattgt cggctgaaga gttttcagtg ttcagcggaa
      841 aactgcagta ctttccaaaa tagaaactgt cttttccaaa tcttcaaaac tgttgtagat
      901 atgaggagag aatcggactc ttcccagcct gtctgcacag atgatttttt tctccttcag
      961 tcccgctgct gtctttccag agtcaagatt ctttttggaa caggcaataa tcccggtctt
     1021 ttcactaaat ttttcagatg caagagaata tccttttttc ttcagcagat ctgaaagaaa
     1081 atcggaaagt tcgtggattc tcattctgac tctgtcaaat ccgatttcag aaagcatcat
     1141 cagagtatgt ctgaaataca cccagtctga gtaatttgct gtggagagct cgaatctctc
     1201 tgctcccggc tgcagctcat ctctgtaagg aagatagacg tcaggattca ctacagaatc
     1261 ggttcctttg aaaaccagtc tgattctatc cagcttttcg gaatttat
//
LOCUS       DAOQGJ010001449         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_82927_length_1307_cov_2.919329, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001449 DAOQGJ010000000
VERSION     DAOQGJ010001449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82927_length_1307_cov_2.919329"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..927)
                     /locus_tag="PK683_18640"
     CDS             complement(130..927)
                     /locus_tag="PK683_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNH10527.1"
                     /translation="MNEMSKYLKLALASVKSRMEYRASFLIFLATLVAFYGAQVMTIG
                     VIVYRFKSIGGWSMGEMAFLYCTLILSQGIVSCIFSGLVDFGTQVRDGSYDRVMIRPL
                     SPILQVTMSGFELTGVTHIILGAAAFIAADQMMGIHWNFLNISYFLLVILGGTLLLAS
                     VRIIIAAVAFWAVNNNSLVHLFVFSSREFLLYPLNIYSSGVRFLLTFIVPLAFINFYP
                     AHYFLQKNPTGFHPALVYATFPVGVVTFLISLLVWKMGQNAYESAGG"
     gene            complement(929..1297)
                     /locus_tag="PK683_18645"
     CDS             complement(929..1297)
                     /locus_tag="PK683_18645"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10528.1"
                     /translation="MKFLPVYFLSFVLFLSFGFFISCLAFFFTEVFPFMILYSALVTL
                     LSGSVIPLNFFPEAFRSAVFWTPFPYLYYFPTAVLISGNLQMEYSELILRYLLETSVM
                     LFLCLRVYFMGLRKVEFAGG"
BASE COUNT          447 a          283 c          285 g          292 t
ORIGIN      
        1 tgagttcttc gatatgattc ttgaattcgg tcaaattctt gtcgaattcc atcttctgct
       61 ctttagaaag cgccatactg gtaataatta tcggaaaatg cagttatttt ctacactgct
      121 tttttcggat tatcctcctg cggactcata tgcattctgt cccattttcc agaccagaag
      181 cgaaatcagg aatgtaacga ctcccacagg aaaagtggcg taaacaagtg caggatgaaa
      241 cccggtcgga tttttctgaa gaaaataatg tgcaggataa aaatttatga atgccagagg
      301 gacaatgaat gtgagcagaa accgcactcc ggatgagtag atgttcagag gatagagcag
      361 aaattcgcgg gaagaaaaca caaaaaggtg caccagggaa ttattgttca ccgcccaaaa
      421 tgccactgct gcaatgatga ttctgacgga agccaaaaga agagtcccgc cgagaatcac
      481 caggagaaaa taggagatat ttaaaaaatt ccaatgaatc cccatcatct ggtctgccgc
      541 aatgaaagct gcagctccca gaatgatatg agtaactccg gtcagttcaa acccgctcat
      601 cgtcacctgc agaataggcg aaagcggtct gatcattacc ctgtcatagg aaccgtctct
      661 cacctgtgtt ccaaaatcca ccagcccgga aaaaatacag ctgacaatgc cctgggaaag
      721 gatgagggta cagtacaaaa aagccatctc tcccatagac catcctccga tgctcttgaa
      781 tctgtataca atgactccga tggtcattac ctgagctccg taaaatgcta cgagagtagc
      841 caggaaaata agaaaactgg cacggtattc cattctggat ttgactgatg caagtgcaag
      901 tttcagatat ttggacattt cattcatttc agcctcctgc aaattccact tttctaagtc
      961 ccataaaata aacccgaagg cacaaaaaca gcatgaccga cgtttcaaga agataacgca
     1021 ggatcagttc cgaatattcc atctgcagat ttccgctgat gagaaccgct gtaggaaaat
     1081 agtagaggta tgggaacgga gtccagaaca cggcagaacg gaatgcctcc gggaaaaaat
     1141 tcagagggat gactgatcct gaaagcagtg tgaccaatgc agaatacagt atcatgaaag
     1201 gaaacacttc tgtaaagaaa aaagcaagac aggaaataaa gaaaccgaaa ctgagaaaca
     1261 gaacaaagga gagaaagtac acaggaagaa acttcaggaa agtctct
//
LOCUS       DAOQGJ010001450         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83052_length_1306_cov_2.853717, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001450 DAOQGJ010000000
VERSION     DAOQGJ010001450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83052_length_1306_cov_2.853717"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PK683_18650"
     CDS             complement(<1..845)
                     /locus_tag="PK683_18650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH10529.1"
                     /translation="MAEEESKKTYEIPAELRLEITKLIHESQSFLRNKKWKEAEQIGK
                     KIEEKLPSSPEPEYIKSYAMFAAKDYANSLKTLLLGLKKSPGHDPSLFLFGLVLSRQN
                     KWDKAAKYFEKACARADYNPFYRYNLASAYYIAGDLEKAVKESETTLRLKENYFKAKI
                     IQIKALKGLGRKKEAYDLAKEMYDRKLELQEITPIYTSLLLETMNDPKEIIAVLSKKN
                     QTSGTEKKMLATAYLKEGDMDKAVLNFKQSVNLDSDTEEEIFHYIYALIFSGKDAEAD
                     KIFGTL"
     gene            complement(967..>1306)
                     /locus_tag="PK683_18655"
     CDS             complement(967..>1306)
                     /locus_tag="PK683_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10530.1"
                     /translation="FRMEKTEPERYTNIISLTAMIPPGKEVSAGFDLGSFILNGNPVY
                     PLKIHSLKEYVLIDEKSFSPYLDQGSVFRQLDNPENQYVIIQKKGSASLYRNCPVKDK
                     DCRQGREHSN"
BASE COUNT          328 a          267 c          247 g          464 t
ORIGIN      
        1 agggttccga aaattttgtc tgcctcagca tcctttcctg aaaatatcag tgcatatata
       61 taatgaaaga tttcctcttc ggtatcagaa tccagattta cagactgctt gaaattcaat
      121 actgccttat ccatatcccc ttctttcaga taggctgttg caagcatctt cttctctgtt
      181 cctgatgtct gatttttttt ggaaagaact gcaatgattt ccttcggatc attcattgtt
      241 tccagcagaa gggatgtata gataggagtt atttcctgca gttccagttt tctgtcatac
      301 atttcttttg ccagatcgta tgcttctttt ttcctgccta atcctttcag ggctttaatc
      361 tgaattatct ttgccttgaa ataattttcc ttgagacgga gggtagtttc cgattctttt
      421 acagcctttt ccagatcccc cgcaatgtag tatgcagaag caagattata tctgtaaaac
      481 ggattataat cagctcttgc acatgctttt tcaaagtatt ttgctgcttt gtcccatttg
      541 ttctgtcggg aaagaacaag tccaaaaaga aaaagagaag gatcatgacc ggggcttttt
      601 ttcaagccga gaagaagggt tttcagtgaa ttcgcgtagt cttttgctgc gaacattgca
      661 taacttttta tatattcagg ttcaggggag gagggcagtt tttcctctat cttttttcct
      721 atctgctccg cttccttcca tttcttattg cgcagaaatg actgagattc atgaatcagt
      781 tttgtaatct ccagtctgag ttccgcaggt atttcataag tctttttgct ttcttcctct
      841 gccaaaaatt ctttttcacc aggcagcagt gcctcttcgg cagttgtttt atgtatgaat
      901 aataacagta aaaaaaggat tcgaatcata gctcgatttt aaacagatgc ctttcaaagc
      961 aagtttttaa tttgaatgct cccgtccctg tctgcagtct ttatctttta cggggcagtt
     1021 tctgtagagt gaagcggaac ctttcttctg aatgatgaca tactgattct ccgggttatc
     1081 cagctgcctg aagactgatc cctgatccag gtagggtgaa aaactttttt catcaattaa
     1141 gacatattct ttcagggaat gaattttcag aggatataca ggattgccgt tcagaataaa
     1201 agagcccaga tcaaatccag ccgacacttc ttttccggga ggaatcattg cagtcagtga
     1261 tattatgttc gtatatcttt ccggttccgt tttttccatt ctgaat
//
LOCUS       DAOQGJ010001451         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83057_length_1306_cov_2.766587, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001451 DAOQGJ010000000
VERSION     DAOQGJ010001451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83057_length_1306_cov_2.766587"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PK683_18660"
     CDS             complement(<1..777)
                     /locus_tag="PK683_18660"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10531.1"
                     /translation="MQELERLLGPKLREIFTHFPILALVGARQSGKSTLIRQIFGKDM
                     ETFVFDPVQDLANARKDPDFFLQNHPAPLFLDEVQYAPELLPALKRKVDTEGRKGMYI
                     LSGSQNFSVMKNISESLAGRVYIQELYPMCERELDEKISSPSFLKEWLESGGTLEYSN
                     ILKFFTEIPKPKEPLLNRIWKGGFPGLLHIPDSFFSGFWNSYVQTYIERDVRLLANIE
                     SLQSFGNFFSLLSSLTANEINYNELGRELGIDRKTAVHWAG"
BASE COUNT          376 a          265 c          265 g          400 t
ORIGIN      
        1 tcctgcccag tggaccgctg tctttctgtc aattccaagc tctcttccaa gttcattata
       61 atttatctca tttgctgtca aagaagaaag cagactgaag aaatttccga aagattgaag
      121 agactcaata tttgccagca gtctgacatc cctttcaatg taagtctgaa cataagagtt
      181 ccagaatccg ctgaaaaaag aatcagggat atggagcagt ccaggaaaac cccctttcca
      241 gattctgttc agaaggggtt ccttcggttt tggaatttca gtgaaaaatt taagaatatt
      301 agaatattct aatgttcctc cggactccag ccattctttc agaaaagacg gagaggaaat
      361 tttctcatcc agttctcttt cacacatcgg atacagttcc tgaatataaa cacgtccggc
      421 aagagattct gaaatatttt tcattacaga aaagttctga gaacccgata gaatgtacat
      481 tcctttccgg ccttccgtgt ccacttttct ctttaaagca ggcagaagtt caggtgcata
      541 ttgcacctcg tcgagaaaca gaggtgcggg atggttctgc agaaaaaaat cgggatcttt
      601 tctggcattt gcgaggtctt gaacaggatc gaaaacgaat gtttccatat cttttccgaa
      661 aatttgccgg attagagtgg attttcccga ctgtcttgct ccaaccagtg ctaaaattgg
      721 gaaatgggta aaaatttccc tcaatttagg ccccaatagt ctttcgagct cctgcatatt
      781 tgtcattttg ggagtgggaa tcccaaatat caagattttt ttgggaggag attgctgaat
      841 gaaaactgcc gaaacgcata aactttttcg agtcaaagat ctccgctgct cagtcagcag
      901 cattacccgt ttccggaata tttcagcatt ggcagcctgc tgtcggaata aagtgcaata
      961 atttgcttac tttaaatgtt cgtctttgcc agtcagaggg ataatcgtat ttttaattac
     1021 attttaatta aatcaatgaa gcatcggtta agtgtcattt ttggcttatt ccttatcgga
     1081 tgaaaagcgg ggattacggc tgctgagagg gcttccagta atgctgtcaa gataaggatg
     1141 attcaccaca gatacacacg gattaacaca gatattgcat caaagtatcc gtgttgatct
     1201 gtgcccagcc ttggtaatct ttttaatttt cttcttaagt tgacagcatt aggcttccag
     1261 tctttgcttc atcaaagata ttggcagcca gcttaaaaaa gatatt
//
LOCUS       DAOQGJ010001452         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83081_length_1306_cov_2.286171, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001452 DAOQGJ010000000
VERSION     DAOQGJ010001452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83081_length_1306_cov_2.286171"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK683_18665"
     CDS             complement(<1..618)
                     /locus_tag="PK683_18665"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNH10532.1"
                     /translation="MRIDLSEYSFNLPEELIAVYPAEKRDHSRLFTADKKNGTFRTDF
                     FFYDLGKFLNQGDLLVFNRTKVSRRRVFLRTETGRQHECMFLTEENSEWHCLIRNSAK
                     LKEHQILISSSGIEFHYTNRNGKSFLIPEEKISEDFFEKDGTVPIPPYLKRKAEESDN
                     ERYQTIFAESAGSAAAPTAGLHFTEELKTELENQGIIFADVCLHVG"
     gene            complement(913..>1306)
                     /locus_tag="PK683_18670"
     CDS             complement(913..>1306)
                     /locus_tag="PK683_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002098517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNH10533.1"
                     /translation="IIQDYRLGRWKKLMWNAPFNPLSVLCGGASTKDILENPHTLDLT
                     KKIMQEVLKTAHALNIPLKNSQPDEFIEMTFRMKPYKTSMLLDFENRRPMEIDVILGN
                     TVKFAGQAGLELPHIYTMYCILKMRNYL"
BASE COUNT          338 a          263 c          277 g          428 t
ORIGIN      
        1 accgacgtgc aggcatacat ctgcaaaaat gattccctga ttttccagtt cagtcttaag
       61 ttcttcagtg aaatgaagcc ctgcagtggg ggccgccgca gaaccggcac tttctgcaaa
      121 tattgtctgg tatctttcat tgtcagattc ctccgctttt cttttgagat aaggggggat
      181 gggaactgtt ccgtcttttt caaaaaagtc ctcactgatt ttttcttcgg gaatgagaaa
      241 tgattttccg tttctgtttg tataatgaaa ttctatccct gatgaggaga tcagtatctg
      301 atgctctttc agttttgcag aattccggat caggcagtgc cattctgaat tttcttcggt
      361 aagaaacatg cattcatgct gtctgcctgt ttctgtacga agaaatactc ttcttctgct
      421 tacctttgtt ctgttgaata caagaaggtc tccctgattc agaaattttc ccaggtcata
      481 gaaaaaaaaa tcggttctga atgttccgtt ttttttatct gcagtaaaaa gtctggaatg
      541 atctcttttt tctgcagggt atactgcaat cagttcttcc ggcagattga aactgtattc
      601 ggaaagatcg atcctcatag gaaattttgt ccttatcgat cagtttgtgc ggagaggata
      661 tccagtcaag aaaatatgat caggtttcgc atttactcat tgaagcaacg ttgtaaagaa
      721 aatcttttta agcggggtct tagcggctac caagctagcg ggggttgcaa acccctcgcc
      781 aagtcgggaa gcggctaatc ttcccgatag ggaccaactt tctcctgctt ttcttaaaag
      841 caaacttttg aaaaacggta tttgtaacta cgacgccaaa tactgaagct gggctgccga
      901 aaggctgagc agttacaaat aattgcgcat ttttagaatg caatacattg tatatatatg
      961 aggaagttcc agaccggcct gtccggcaaa ttttactgta tttcctaaaa tgacgtcgat
     1021 ctccattgga cgtcggttct caaaatccag gagcatactt gtcttgtaag gtttcattct
     1081 gaatgtcatt tctatgaatt catccggttg ggaatttttc agaggaatat tgagtgcgtg
     1141 tgctgtcttc agcacttcct gcattatttt ttttgtaaga tccagtgtgt gaggattttc
     1201 aagaatatct tttgtgcttg cacctccgca tagaacagaa aggggattga agggagcatt
     1261 ccacatcagt tttttccatc ttccaagcct gtagtcctga ataatc
//
LOCUS       DAOQGJ010001453         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83211_length_1305_cov_2.067200, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001453 DAOQGJ010000000
VERSION     DAOQGJ010001453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83211_length_1305_cov_2.067200"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PK683_18675"
     CDS             <1..893
                     /locus_tag="PK683_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002089887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNH10534.1"
                     /translation="QEEDKESGLYYYKARYYDASLGRFNSADSLIMPESIQGMNRYMY
                     VAGNPILYNDPTGHKLSSGQSWGLFAYFVGQQYGLSFEQMAGIAIGGRHKEKKKHKKS
                     VEKNFLNNVGFAFDPGYRIYKAGQYLSGVGGKDKLDSRYKSAALGYMLNGQEGAIWGY
                     LYGRSADKKKEMNRRHNQALAIEHTINLVIIAASAGLGAEGVESDTIGKSISNAFKNL
                     GFKSVGARLALGGIIDYGLSNQIKGISEQMAGYDDDTERKIKGTYNMCFYAFTQHALN
                     INDRNADFIGAMCATGIYPL"
     gene            890..>1305
                     /locus_tag="PK683_18680"
     CDS             890..>1305
                     /locus_tag="PK683_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10535.1"
                     /translation="MKFSKLAAKVFYLKMKLCAILFCSIGCFSVKLKGNLSEADYKSR
                     LTVEKYTSINPETNGGYNREFTAVWKRTPKESVSIGMLLNVETNETNDKWFPLYAVWA
                     IASGYSRFLIPYYRKYSGNMVVKSKTGDVRKVYELE"
BASE COUNT          422 a          192 c          325 g          366 t
ORIGIN      
        1 gacaggaaga ggacaaggaa tcaggactgt attattataa ggcacgttac tatgacgcca
       61 gtttaggaag gttcaattca gcagacagtc tgattatgcc agagtctatt cagggaatga
      121 atcggtatat gtatgtggca ggaaatccaa tactgtataa tgatccgact gggcataagc
      181 tgagttcagg gcagagctgg ggattgtttg catactttgt cggtcagcag tatgggctgt
      241 cttttgaaca gatggcagga attgcaattg gaggaaggca caaggaaaag aagaaacaca
      301 agaaatccgt ggagaagaat ttccttaaca atgtaggatt tgcattcgac cctggatatc
      361 gtatatataa agcgggacag tatttgtctg gtgtgggggg aaaggataaa ctggacagtc
      421 gctataagtc agcagcatta ggctatatgc tgaacggtca ggaaggtgca atatggggtt
      481 atctttacgg ccgttccgca gacaagaaaa aggagatgaa cagacggcat aatcaggcct
      541 tggctattga gcatactata aatcttgtaa ttattgccgc aagtgcggga cttggtgcag
      601 aaggagtgga aagtgatact attggcaagt ctattagtaa tgcttttaaa aatcttggat
      661 tcaaatctgt aggggctaga ttagctttgg gaggaattat tgattatggt cttagcaatc
      721 aaataaaagg aatctcggag cagatggctg gctatgatga tgatactgaa cgaaaaatta
      781 aaggaactta taatatgtgt ttttatgcgt ttactcagca cgccctgaat attaatgaca
      841 gaaatgcaga tttcatcggt gctatgtgtg ccacagggat ttacccgtta tgaaattttc
      901 caaattggct gctaaagttt tctatttgaa aatgaaactc tgtgctattc ttttttgttc
      961 tattggatgc tttagtgtaa aattgaaggg aaatctttca gaagctgact ataaatcaag
     1021 gctgacagtc gagaaataca cctcgataaa tcctgaaact aatggaggtt ataacaggga
     1081 atttacagca gtctggaaaa gaacaccgaa agagagcgtt tctataggaa tgttgctgaa
     1141 tgttgagacg aatgaaacaa atgacaaatg gtttccacta tatgcagtat gggcgatagc
     1201 atcagggtat tccagatttt taattccgta ctacagaaaa tattcaggta atatggttgt
     1261 aaagtctaaa actggagatg ttcgaaaagt ctatgaatta gaatt
//
LOCUS       DAOQGJ010001454         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83212_length_1305_cov_2.053600, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001454 DAOQGJ010000000
VERSION     DAOQGJ010001454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83212_length_1305_cov_2.053600"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PK683_18685"
     CDS             <1..290
                     /locus_tag="PK683_18685"
                     /inference="COORDINATES: protein motif:HMM:NF037629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNH10536.1"
                     /translation="APRPHNSGHFSIDSGFCSQFDLQLFSLVFESLPFQPKDSSPCIM
                     YNIIGEEFYRSPDSWIRDRAQGDEWRLHLYQKNLPRPGRKMGHLNVLRKHS"
     gene            508..1281
                     /gene="proC"
                     /locus_tag="PK683_18690"
     CDS             508..1281
                     /gene="proC"
                     /locus_tag="PK683_18690"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNH10537.1"
                     /translation="MEKEIIGIAGFGNMGSAIWKALHGERDLLIYDPYISQFPSDSRH
                     TESLNNFINKSDIIILCIKPDKIEGFLSHIKTPKKIISIAAGITLSSLKKSLPEGSSA
                     ARLMPNLPLTVGQGAIGYISDSALKEKTVELFSKLGLLVEVAKEELMNSVTGLSGSGP
                     AYVFSFINALAEGGVKSGLSYDQALRLALQTVKGSAAYMEEELSKDRTVHPGTLRNRV
                     TSPGGTTIYGLEKLEQNSFHYAVMNAVFAAFKRSEELGK"
BASE COUNT          404 a          268 c          299 g          334 t
ORIGIN      
        1 tcgcacccag acctcataat tcggggcatt tcagcataga ttcaggattc tgctcgcagt
       61 ttgatctgca gctgttcagc ctcgtttttg aaagccttcc ttttcagccg aaggactcaa
      121 gtccctgcat catgtacaat atcataggag aagaatttta ccgatctccg gacagctgga
      181 tcagagacag ggcacaaggg gatgaatgga gacttcattt atatcagaaa aacttaccga
      241 gacccggaag aaaaatggga catctgaacg tactgaggaa acattcctaa aatcgaacac
      301 aaataatgat actgtatttc aggtcaaaca tcccgttcac aggaaattca gcattaaaaa
      361 atctgacaga acagattatc aggagcttaa aacatagtaa gcttatacag taactgcact
      421 tatcctaatt tctgatcata cgcgggagtc tgcgcttctg ccggcaggaa attggagaaa
      481 taaaaacaaa tatcgcgagg aaataaaatg gaaaaagaaa tcataggcat tgcgggtttc
      541 ggcaatatgg gttctgcaat ctggaaggca ctgcacggag agagggatct gcttatctac
      601 gatccgtaca tctctcagtt cccttctgat tccagacata cggagagtct taataatttt
      661 atcaataaat cagatatcat tattctgtgt ataaaaccgg ataaaattga gggatttctt
      721 tctcatatta aaactccgaa aaaaatcatt tccattgcgg caggcatcac gctttcttcc
      781 ttaaaaaaaa gtcttccgga gggaagctcc gctgcacgac ttatgcctaa cctccctctt
      841 acagtcggtc agggggcaat aggatatatc tcagacagtg ctctaaagga aaagacagtt
      901 gaactttttt ccaagctggg actgctcgtg gaagtagcta aggaggaact gatgaattct
      961 gtcactgggc tttccggttc aggacctgct tatgtattca gttttataaa tgcgctggcc
     1021 gagggaggcg taaaaagcgg actcagttat gatcaggctc ttcgtcttgc tcttcagaca
     1081 gtgaaaggca gtgcggccta tatggaggaa gagctttcga aagacaggac agtccatccg
     1141 ggaacattga gaaacagggt cacatccccc ggaggcacta caatttacgg attggaaaaa
     1201 ctggaacaga attcatttca ttatgcagtg atgaatgctg tatttgctgc ctttaagaga
     1261 tctgaggagc tgggaaaata aatggtggcg cggacaggat tcgaa
//
LOCUS       DAOQGJ010001455         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83231_length_1305_cov_1.712800, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001455 DAOQGJ010000000
VERSION     DAOQGJ010001455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83231_length_1305_cov_1.712800"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PK683_18695"
     CDS             <1..374
                     /locus_tag="PK683_18695"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNH10538.1"
                     /translation="GVVKLMGYLDDYAYFIDDEFLIAPLNPEDPSDDWRMNHCCEPNC
                     GIRGQIIFTALRDIEPGEELTFDYCMTESDEEYSMNLSCSRETCRKKFTGADWKNPEL
                     QAKYRGYFSLYIEQKISRLSD"
     gene            436..1068
                     /locus_tag="PK683_18700"
     CDS             436..1068
                     /locus_tag="PK683_18700"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH10539.1"
                     /translation="MNINNDSAEKSVNIRKRLETEKLLILPNVISSVRVFLLPFFLFF
                     GRKYSADHSILHLVYIYLLFFIMSASDILDGFVARKLRQETVFGKYMDPVCDKITAIL
                     SLLMLSLYYSFPWILFYLVFLREILGTAVGTVLFFRYKVQGNPNFAGKLTVWASGFTI
                     LWYISSYEISVPEQFLHIPAWLLFSVYVYAALGYLISFRNEILKADQNSE"
BASE COUNT          360 a          258 c          247 g          440 t
ORIGIN      
        1 ccggtgttgt caaactcatg ggctatctcg atgactatgc atatttcatt gatgatgagt
       61 ttctgattgc gcccctgaat cctgaagatc caagcgatga ctggagaatg aatcactgct
      121 gtgaaccgaa ctgcggaatt cgcgggcaga ttatttttac tgcactcaga gacattgaac
      181 ctggggaaga acttaccttt gactactgta tgactgaatc tgatgaagaa tattcaatga
      241 acctttcctg ctcacgggaa acctgcagaa aaaaatttac aggtgcagac tggaaaaatc
      301 ctgaacttca ggctaaatac agaggatatt tttcattata tattgaacaa aagatttcaa
      361 gactgtcaga ctgaaaataa ttttcttacg tctgccttct ttggaaggaa ttcaatgctc
      421 cggaaatgat tcagaatgaa cataaataat gattctgctg aaaaatccgt aaatatcaga
      481 aaacggctgg aaacagaaaa acttctgatc ctgccgaacg tcatttcttc agtcagagtg
      541 tttcttcttc ctttttttct tttctttgga agaaaataca gcgcagatca cagtatttta
      601 catctggttt atatctatct gctgtttttt attatgtctg catctgatat tctggacggg
      661 tttgtcgcaa gaaagctcag gcaggaaacc gtattcggaa agtatatgga tcctgtctgc
      721 gacaagatca ctgcaatact ctcacttctc atgctctctc tttattactc atttccatgg
      781 atactgtttt atcttgtctt tttaagagag attctgggaa ctgctgtcgg gactgtcctt
      841 tttttcagat acaaggtgca gggaaatccc aatttcgcag gaaaattgac tgtatgggca
      901 tccggtttta ccatactctg gtacatttct tcctatgaga tttcagttcc tgaacagttt
      961 ctgcatatcc ctgcctggct ccttttttca gtctatgttt atgctgctct gggatattta
     1021 atatcatttc gtaatgaaat actgaaagcg gatcaaaact ctgaatgacc cgcttgaaat
     1081 ctgtatgctt taaacgcttt aatctctttc tgcagattta ttcgatttgc ctgatgtttt
     1141 cagaaaatga cagtgtccag acagcaatat cgtctttgtg taacatgagt gatttattct
     1201 gaaatcttcc tcagcagaag acctttttct tttctgacgg gatcttcatt tttaaatctg
     1261 gatttcagaa tttggaaaaa ctcttcatct ttttcagccg acagt
//
LOCUS       DAOQGJ010001456         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83256_length_1304_cov_3.839872, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001456 DAOQGJ010000000
VERSION     DAOQGJ010001456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83256_length_1304_cov_3.839872"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PK683_18705"
     CDS             complement(<1..209)
                     /locus_tag="PK683_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10540.1"
                     /translation="MKKLRKSISTQIPAKDDSFNFFIMTKSLNRLTCQVQEKPIWFPG
                     ADFLSTEDLSHTFITLNIICTKMIFV"
     gene            241..732
                     /locus_tag="PK683_18710"
     CDS             241..732
                     /locus_tag="PK683_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10541.1"
                     /translation="MNSNYALKEIRTDRSVSTLEKEHVFRIPCENFADIAYDVNNDYP
                     AGILKTDLFKNRCRNFFLLIETEDEKISVKIDGMRLNDSVKYRYEYAVIRLQSLNPPV
                     RKYEFPKKPETAYAYYTEPSASPREIRYTVKTRILILNEKTESAGTIETVSDVGASLY
                     LLN"
     gene            complement(729..>1304)
                     /locus_tag="PK683_18715"
     CDS             complement(729..>1304)
                     /locus_tag="PK683_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10542.1"
                     /translation="LIIKKLNESEKEIYRAHPEMCSGPCSEKSDTALYRVSFSGTLKK
                     GLNRISAGYRQILSHRETKYGYASSSKWASGLSYELWPLKEWKLASDFQMNVRFTVPS
                     PGFFSSAGTVCTGEDLNFQKYPFSILKKEYSKSETGTSGMPERTKPNHFYSLKEKRKI
                     SEKDGKILYEVSFGNSFPDRISCSYGHTKEH"
BASE COUNT          381 a          268 c          260 g          395 t
ORIGIN      
        1 acaaagatca ttttagtgca gataatattt aatgttatga aagtgtgact caaatcttcc
       61 gtggacagga aatctgctcc gggaaaccaa atcggttttt cctgaacttg acaggtcagc
      121 ctgttcagtg attttgtcat tatgaaaaaa ttaaaactgt catcctttgc cggaatctgt
      181 gtgcttatgc tttttctgag ttttttcaat tcctgcattt ctgcggtata ttcacccggg
      241 gtgaacagca attacgctct gaaggaaatc agaacggaca gatctgtttc cactctggaa
      301 aaagaacatg tgttcaggat tccatgcgaa aattttgcag atatcgccta tgatgtaaac
      361 aatgattacc ctgcgggaat attaaaaaca gatctcttta aaaataggtg caggaatttc
      421 ttcctgctca ttgaaacgga agacgaaaag atttccgtaa agatagacgg tatgagactg
      481 aatgattcag taaaatacag atatgaatat gcagtcatcc gtctgcagtc actgaaccct
      541 cctgtccgca aatatgagtt tccgaaaaaa ccggaaaccg cttatgcata ttacactgaa
      601 ccttccgcaa gtcccagaga gatcagatac acagtgaaga caagaatcct tatcctgaat
      661 gaaaaaacag aatcagccgg gactattgaa acagtctcag acgtcggcgc atcattatat
      721 ctgctgaatt agtgttcctt agtgtgaccg taggaacagc tgatcctgtc cggaaatgaa
      781 tttccgaaac tgacttcata aagaatttta ccgtcttttt cactgatttt tctcttttcc
      841 ttcagactgt aaaaatggtt gggttttgtt ctttcaggca ttcctgatgt tccagtctca
      901 gacttactgt attctttttt cagtatggaa aaaggatact tctgaaaatt caaatcttct
      961 ccggtacata cggtgcctgc tgaactgaaa aaaccgggac ttggaacagt aaaacggaca
     1021 ttcatctgaa aatcagaagc cagtttccat tccttcagag gccagagttc gtagctgagt
     1081 ccggacgccc atttacttga ggaagcatat ccgtattttg tctctctgtg agaaaggatc
     1141 tgtctgtatc ctgcactgat tctgttcaga ccttttttca gcgttccgga aaaacttact
     1201 ctgtacagtg ctgtatcgga tttttctgaa cagggaccgg aacacatttc aggatgagcc
     1261 ctgtagatct ccttctcgga ttcattaagt tttttgataa tcaa
//
LOCUS       DAOQGJ010001457         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83268_length_1304_cov_3.104884, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001457 DAOQGJ010000000
VERSION     DAOQGJ010001457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83268_length_1304_cov_3.104884"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1304)
                     /locus_tag="PK683_18720"
     CDS             complement(<1..>1304)
                     /locus_tag="PK683_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004497075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH10543.1"
                     /translation="YRFSLGIFFALFTSLFNAVSLTVLIPLFDALGSTQKQKFQMEFT
                     YPEKKILYKEMVMGYDSLDGLEKIKRVLINVKQESNDYMKDKEPKEIVWSVCKIIIPL
                     SVLKLLFYLASVYCIATAGYMAVRDIRQKLYDKMQTLPLTYFYKEKTGMLMSRIINDA
                     EIIAAVISSNLRDAVINFFYLITHLVILLYLNAELLLIAAVTVPVIMYPVTLFTKKIA
                     KSTQKLQERIADLNGIVHEMISGIRVIRSFRMEQSMEHNFQAINDKASHRNFKGQFYL
                     QMAPSLVEFTSSLVVIGFFWIGASLIYSGRFTQGEFMAFLLTLMFLLRPVTQLSQMAG
                     KMTQARSAGTRIFEIIDREIFEDHSTLGSGLKNRGLQNSIVFENLHFKYPDTVQEVLK
                     GIDLKVKIGETVALVGSSGSGKSTLMDLIPRFYEPTDGRILM"
BASE COUNT          392 a          294 c          268 g          350 t
ORIGIN      
        1 ccatcagaat cctgccgtcc gtcggttcat aaaaacgggg aataagatcc atcagagtag
       61 actttccgct tccgcttgat ccgaccaatg ctacagtctc ccctatcttt accttgagat
      121 caatcccctt cagtacttcc tgaacagtat ccgggtattt gaaatgaaga ttttcaaaga
      181 caattgaatt ctgaagcccc ctgtttttca gaccggagcc taaagtgctg tgatcctcaa
      241 atatttctct gtcgattatc tcaaagatac gtgtgccggc agacctcgcc tgagtcattt
      301 ttcctgccat ctgagaaagc tgagtcacag gtctcaacag aaacatcaga gtaaggagaa
      361 atgccataaa ctctccctga gtaaatcttc cgctgtaaat cagacttgca ccaatccaga
      421 aaaaaccgat gacaacaagg gaagaagtaa actctacaag actgggtgcc atctgcagat
      481 aaaactgccc cttgaagttc ctgtgagatg ccttgtcatt gatcgcctga aaattgtgct
      541 ccattgactg ttccatccgg aatgaacgga ttaccctgat ccctgaaatc atttcatgaa
      601 caattccatt cagatcggca attctttcct gcagcttctg agtggacttt gcaatttttt
      661 tggtaaatag ggtgacagga tacataatca cgggcacagt cactgctgca atcaggagga
      721 gctccgcatt cagataaaga agaatgacaa gatgagtgat cagatagaaa aaattgatga
      781 ccgcatcccg cagattgctt gagatgacgg cagctatgat ttccgcatca ttgatgatcc
      841 tgctcatcag catccccgtc ttttccttat agaaataagt caaaggcagg gtctgcatct
      901 tatcatagag tttctgccgt atatctctga cagccatata gccggcagtg gcaatgcagt
      961 agacagatgc cagataaaaa agaagcttga ggacagagag aggaatgatg atcttgcaga
     1021 cagaccagac aatttccttg ggttctttgt ccttcatata atcattggat tcctgcttta
     1081 cgttgatgag gactcttttg attttttcaa gaccgtccag agaatcatag cccataacca
     1141 tttctttgta cagaatcttt ttttcgggat aagtgaattc catctggaat ttctgcttct
     1201 gagtcgaacc cagtgcatca aacagaggaa tgagaacagt cagagagaca gcattaaaaa
     1261 gtgaggtaaa aagggcaaaa aatataccta acgaaaacct gtat
//
LOCUS       DAOQGJ010001458         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83357_length_1303_cov_3.606571, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001458 DAOQGJ010000000
VERSION     DAOQGJ010001458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83357_length_1303_cov_3.606571"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1303)
                     /locus_tag="PK683_18725"
     CDS             complement(<1..>1303)
                     /locus_tag="PK683_18725"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10544.1"
                     /translation="NYSREKGELEFLDFGSASFLFRESQSVLNVNQFQGSAAYISPEQ
                     TGRMNRTVDYRSDYYSLGITFYQMLSGVLPFESNEIMEMIHFHIAVNPVPLKERQKSV
                     PESLSDLVMKLMKKNPEDRYQSAEGILYDIREIRDNFEKGRSRKVQIGRMDFSSKFQI
                     PERLYGREKEIERIIECFNDMYNYRKNEMILVSGASGMGKSALIQEVYKPVTGRKGYF
                     IQGKFDQYKRDLPYNAFIQAFGELVSQILTEDNISVSRWKADILAAAGENAFVLTELI
                     PELELLIGVQPKKNNDLPAQESMNRFIFSVRAFVGVFAKFRHPLALFLDDIQWADSSS
                     VRLIRFLYTDPSIESFLLICSFRENEIDSSHPFSVMLGDLEKEKQNWKSLKVGALSVD
                     DIGRLAADTLNCSINEVREIAETVHSRTGGNPFFVIELMKSL"
BASE COUNT          363 a          324 c          231 g          385 t
ORIGIN      
        1 gagtgatttc ataagttcaa ttacaaaaaa tggatttcct ccggttctgg aatggacagt
       61 ttccgcaatc tctctcactt catttatgct gcagttcaaa gtatcagctg ccagtcttcc
      121 gatatcatcc acggaaagag cgcctacttt cagactcttc cagttctgtt tctccttttc
      181 caagtctcca agcatgactg aaaacggatg agaagaatcg atctcatttt ctcgaaagga
      241 acagatcagg agaaaactct cgatagacgg atcagtatac aggaatctga tcagacggac
      301 agaagaagaa tcagcccatt gaatatcatc caggaaaagc gccaaagggt gacgaaattt
      361 cgcaaacacc cctacaaagg ctctcactga aaaaataaat ctgttcattg attcctgtgc
      421 aggcagatca ttattttttt taggctgaac tccaatcaga agttccagtt caggaatcag
      481 ttcagtaaga acaaaggcat tttctcctgc tgctgccaga atatcagcct tccatctgct
      541 cactgaaata ttgtcttctg tcagtatctg cgagaccagt tcaccgaagg cctgtataaa
      601 agcattgtaa ggaagatctc ttttatactg atcaaatttt ccctgtataa aatatccttt
      661 tcttccggtc acaggcttgt acacttcctg aatcagtgca gacttgccca tccctgaagc
      721 acccgagaca agaatcattt cattttttct gtaattgtac atatcattga agcattctat
      781 gattctttcg atttccttct ctcttccata cagcctttcg ggaatctgaa actttgatga
      841 aaagtccatc ctccctatct gcacttttcg ggatctgcct ttctcaaaat tatcacgaat
      901 ctcccgaata tcatacagta tcccttctgc actctgataa cggtcttctg gatttttctt
      961 catcagcttc attaccagat cagacaggct ctccggcact gatttttgcc tctccttcag
     1021 cggaactgga ttcacagcta tgtgaaaatg aatcatttcc attatctcat tggattcaaa
     1081 cggaagaact cccgacagca tctgataaaa agttatcccc agtgaatagt agtcggaccg
     1141 gtaatccact gtccggttca tgcgtcctgt ctgttcaggg gatatgtatg ccgcgcttcc
     1201 ctgaaactga tttacgttca gtaccgactg agattcacga aacagaaaac tcgcactccc
     1261 gaaatccaga aattcaagct cgcctttctc ccggctgtaa ttc
//
LOCUS       DAOQGJ010001459         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83675_length_1301_cov_1.443018, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001459 DAOQGJ010000000
VERSION     DAOQGJ010001459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83675_length_1301_cov_1.443018"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PK683_18730"
     CDS             <1..623
                     /locus_tag="PK683_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10545.1"
                     /translation="FSGFTVTSTEKSYLEDRDYIRNEEWFLRYLEKPNNVEPFPFDES
                     ILEGAKVEKIEKTYYTVYNIQYGKPLDDCSVENNSRVVFVNKKTRQAYSIQGLYKEDL
                     VSHGNLFLIRFIHRTMSATAAVLTEAISFIFFLRTRLFLFLTETRTAKVCAITEMWSQ
                     WNWEERPVMTRRTRPFICISVCLSVLPPLKVKRLLNRLITLILFLL"
     gene            730..>1301
                     /locus_tag="PK683_18735"
     CDS             730..>1301
                     /locus_tag="PK683_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10546.1"
                     /translation="MIISGFYREHHERRLDTLRKFYIANGIILTAIIVSEFFRLSHIP
                     GVRILLLFKMFMAVLTIGYVFIGSEIQRSTGTVIVGTLLAFTYCMMADFVGSENIPFN
                     FDVFTLQVGLILLSCLGLSGMKLFYSILSTLPVLFVYGAVVYSRAEMHYSGLFLFIFI
                     IVGIFISGTFHDHYSMEREMAELIDYWKRYS"
BASE COUNT          363 a          265 c          277 g          396 t
ORIGIN      
        1 tattttcggg attcactgtc acgtccaccg aaaaatccta tcttgaagac agagattata
       61 tccggaatga agaatggttt ctcagatacc tggagaagcc gaacaatgta gagccttttc
      121 ctttcgacga aagcatcctg gaaggcgcga aagtggagaa aatagagaaa acttattata
      181 ctgtctacaa tattcagtat gggaaacctc tcgatgactg cagcgtggaa aacaattcaa
      241 gggttgtctt tgtgaataaa aagacaagac aggcttattc cattcaggga ctctacaagg
      301 aggatcttgt cagtcatgga aaccttttcc tgattcgatt tatacatcgt acgatgtctg
      361 caactgctgc tgtcctaaca gaggcaatct cctttatttt ctttttgagg acaaggctct
      421 ttctgttcct cacagagaca aggacagcaa aggtctgtgc aattacggaa atgtggagtc
      481 agtggaactg ggaagagaga cccgttatga caaggagaac aaggccattt atctgtatct
      541 ccgtctgcct ttctgttctc ccccccctga aggtgaaacg cttgctgaac cgattgatta
      601 cactcattct ctttttactg tgatcaggca ggagaaggac aatgtaaata tagaaagata
      661 tttcgacaac gggattcctg aaaagtacag agatcaatgg gacaaactcc acggaggtca
      721 gtccatcaaa tgatcatctc gggattttac agggaacatc atgaaagaag gcttgatact
      781 ctccgaaagt tctatattgc aaacggaatc atactgactg caatcatagt ttccgaattc
      841 ttccgcctct cgcatattcc gggagtccga attcttcttc tgttcaaaat gtttatggca
      901 gtgcttacaa taggctatgt atttatcgga tctgaaattc agagaagtac aggcaccgta
      961 attgtaggca ctcttctggc tttcacatac tgtatgatgg ctgattttgt cggatccgaa
     1021 aatatccctt ttaatttcga tgtgtttact ctgcaggtag gactgatcct gctttcctgt
     1081 cttggtcttt ccggaatgaa acttttttat tccattcttt ccactctccc ggttcttttt
     1141 gtctacggtg cagttgtcta ttccagggct gagatgcact attcaggact tttccttttt
     1201 atattcatta ttgtagggat atttatcagc ggaacatttc atgatcatta ttcgatggaa
     1261 agagaaatgg cagagctgat cgattactgg aaaagatatt c
//
LOCUS       DAOQGJ010001460         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_83907_length_1299_cov_2.026527, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001460 DAOQGJ010000000
VERSION     DAOQGJ010001460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83907_length_1299_cov_2.026527"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..325
                     /locus_tag="PK683_18740"
     CDS             86..325
                     /locus_tag="PK683_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10547.1"
                     /translation="MNNGANLRKIDFSKNSESFDNKTAVSDSKKSILLILKDSNYYTK
                     CGFYLLVCKKRSNAEQKTEELRNVKTISTLIVYLD"
     gene            complement(756..>1299)
                     /locus_tag="PK683_18745"
     CDS             complement(756..>1299)
                     /locus_tag="PK683_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10548.1"
                     /translation="AIRKTVGISGDQLLSSAVADFKARKSSLMANIKKIRVTGYKIMK
                     PENPDEFDQFLESGISFDLHPRSVENYDQIPSIGKIFQSDVKPYLNKVSGYFDGPGDS
                     VCFLFDTGTYRWTGPGWKAVFFPNSRQFQFISKGWVITEWANGKKRLRSPDKASVLFL
                     NRKEKKYSEKPGAKSAPKNR"
BASE COUNT          445 a          259 c          230 g          365 t
ORIGIN      
        1 gaactttttg tttcttcatt atacagaaga aatacaacag gatatgaaat gtttataaat
       61 aaaacacttc gttttaaaaa ccgacatgaa taacggtgca aatttaagaa aaatagattt
      121 cagtaaaaat tcagaatctt tcgataataa aacagcagta tctgattcaa aaaaaagcat
      181 actgctcata ttaaaggatt ccaattatta tacaaaatgc ggattttatc tgcttgtctg
      241 taaaaaacga agcaatgctg aacaaaaaac agaagaactg cggaatgtaa aaacaataag
      301 tacattaata gtatatttag actgaccaat tgatttcaaa aactctccgc agaacttccg
      361 caggtatgcg ttcaaagatg aactgaatct gataatacat aaattaaata tattatatat
      421 aatacttata ttattaatta tagcaggccc aatgcagtat tgatacaaag cagattattg
      481 ctgttaatta atgtgagatc aagcctgccg agacagcttc aaatttcacc gagtcagccc
      541 cagctttaaa ggctagggaa aaggctgcat cgtatgccga aaaatcggac gggaatggct
      601 cccaagccct cttctatttc taaaacccgc aagggcgggg cggaaacgga acagatgctc
      661 cgctgttcag aggccagcca gattaaaatc tgagcacaat gaaaggataa acaacaaagc
      721 gctttcattc attaaattat aaaatcatct ataatttatc tgtttttcgg agccgacttg
      781 gctcccggtt tttcagaata ctttttctcc ttacggttca gaaatagcac agaggcttta
      841 tccggagaac gcagtctttt ttttccattt gcccactcgg taattaccca ccctttcgaa
      901 ataaattgaa actgtcggct gttgggaaaa aagacagctt tccagccggg ccctgtccac
      961 cgatatgttc cagtatcgaa aagaaagcaa acggaatctc ccggcccgtc aaaatacccg
     1021 gagactttat tcaaataagg cttcacatct gactgaaata tttttccgat ggaaggaatc
     1081 tgatcataat tttctacaga acggggatgc agatcaaatg aaatgccaga ctctaaaaac
     1141 tgatcaaatt catcaggatt ttccggtttc attattttat atcctgtcac tctgatcttt
     1201 ttaatatttg ccatcaggga ggattttctg gccttaaaat cagcaaccgc agaagaaaga
     1261 agctggtctc cggaaatccc gactgtcttc cttattgcc
//
LOCUS       DAOQGJ010001461         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84019_length_1298_cov_1.993564, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001461 DAOQGJ010000000
VERSION     DAOQGJ010001461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84019_length_1298_cov_1.993564"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PK683_18750"
     CDS             complement(<1..836)
                     /locus_tag="PK683_18750"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNH10549.1"
                     /translation="MISETEPQKTRFDAPDILRGLAVVCMIFSNIAGESLNAPHPFVL
                     RIFGSLAAPFFVFLSGYMLSYAYEKYGDSLGYFLKRGLFLIGLSSAIDIFSWKSFPML
                     AMDVLYLIGLSTVLMYWLLRRSTFSRFIILSCAVLAAPALQYIFGYENSNNYVNLYIN
                     EKFNSGFSPDYAVILKRWMIDGWFPFFPWISFSLLGTVVYDRQRNSEYSEDRVYYYIA
                     SFIGLLGILIWDFEPKLEIRDGYTELFYPPTVSYFLCYFGLVLVIFQWVSGRTFGLSM
                     SL"
     gene            complement(836..>1298)
                     /locus_tag="PK683_18755"
     CDS             complement(836..>1298)
                     /locus_tag="PK683_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10550.1"
                     /translation="VIYMNCDTGRIGDCDPENCSRLELYKNLLLNSLVYEYTVDTYPV
                     QVVDCINLNQIDYDLKIRNVNSMALIKFGFYRSICRGVNRKLLHKFEVRLDNDPELCS
                     VIENSIADCFFGLIHSNSHSYSFRKYMERMEDRGFKVPKSMVEKINSYLNK"
BASE COUNT          421 a          271 c          265 g          341 t
ORIGIN      
        1 aagagagaca ttgaaagacc gaaggttctg ccggataccc attgaaagat cacaagcacc
       61 agcccgaaat aacataaaaa ataggaaact gtcggaggat aaaaaagttc agtgtatccg
      121 tctctgatct caagcttggg ttcaaaatcc cagatcagta tcccaagaag tccgataaag
      181 gaagcgatgt aatagtacac tctgtcctcg gaatattcag aatttctctg cctgtcatag
      241 acgactgttc caaggagaga aaaggaaatc cagggaaaga acggaaacca tccgtcaatc
      301 atccatcttt tgagaatgac agcataatca ggagagaatc cagaattgaa tttttcatta
      361 atatataagt ttacatagtt atttgaattt tcatatccga aaatgtactg cagtgcgggt
      421 gccgctagaa ctgcgcatga aaggataatg aatctgctga acgtgctcct tctcagaagc
      481 cagtacatta gaacagtgga cagccctatc agatacagaa catccattgc aagcattgga
      541 aatgacttcc aggaaaaaat gtctatcgca gaggagagac cgatcaggaa aagtcctctt
      601 ttcagaaaat agcccagact gtccccgtat ttttcatatg cataagaaag catatatccc
      661 gacagaaaaa caaaaaaggg agcagcaagt gatccgaaaa ttctgagaac aaacggatga
      721 ggtgcattca gtgactcgcc tgctatattc gaaaatatca tgcatacgac agccagtccc
      781 cgaagaatgt caggagcatc gaatcttgtt ttctgcggtt cagtctctga tatcattatt
      841 tattcagata ggaatttatt ttttccacca tgctcttagg caccttgaaa cccctgtctt
      901 ccatcctctc catatacttt cggaatgaat aggaatgaga gttggaatgg atcagtccga
      961 aaaaacagtc tgctatggag ttttcgatca cggagcagag ttcaggatca ttgtcgagac
     1021 ggacttcaaa tttatgcagc agtttcctgt tgactcctct gcagatcgac ctgtaaaaac
     1081 cgaatttaat cagggccatt gagttcacat ttctgatttt cagatcatag tcaatctgat
     1141 tcaggttgat gcagtccact acctgaaccg gataagtatc cacggtatat tcatagacaa
     1201 gactgttcag aagaagattc ttgtaaagtt ccagacggga gcagttttct ggatcacagt
     1261 ctcctattct tcctgtatca cagttcatat aaatgact
//
LOCUS       DAOQGJ010001462         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84062_length_1297_cov_4.161836, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001462 DAOQGJ010000000
VERSION     DAOQGJ010001462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84062_length_1297_cov_4.161836"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="fliN"
                     /locus_tag="PK683_18760"
     CDS             <1..523
                     /gene="fliN"
                     /locus_tag="PK683_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020783319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNH10551.1"
                     /translation="LVKIAMNLNIETVAVFKVYYIMSQATAEEILALFKKGPQKTGGT
                     GGGINISVPAANTQSSISSQQNSVGIKGIAFPSLSNAAQPSGATNLNLLMDVQMSVTV
                     ELGRTKMYIKDILGLGEGSIIELDKLAGEPVDLLVNGKLIAKGEVVVIDENFGVRVTD
                     IVSPTDRIKSEGK"
     gene            523..1287
                     /gene="fliO"
                     /locus_tag="PK683_18765"
     CDS             523..1287
                     /gene="fliO"
                     /locus_tag="PK683_18765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNH10552.1"
                     /translation="MPLRKILLSFGLILTLSVSEVNSQSRTEELDKELRKELGQEKKE
                     KQTSEKNENTEKEKSEKETKTSDKKPDKKESPEKNPVLERYDSSKEDAPSLLWILLKV
                     FFVLAVLVGVFYYLLRLISRNRNLKFPVQGAMRVLSTLPLGPNKELQLVEVSGMLVLV
                     GVTDNSISLIKEIDSPEIKQKIFSIKESFEPPKENFLELIVQTVKEDIQSVSHKKQKI
                     QIDDEHEEILDELKSRQLDRLEKLKKERQSISKKKD"
BASE COUNT          439 a          267 c          284 g          307 t
ORIGIN      
        1 gctcgtcaag atagcgatga atctgaacat cgagaccgtt gcagtcttta aagtatacta
       61 tattatgtct caggcgactg ctgaagaaat tctggctctg ttcaaaaaag gtcctcaaaa
      121 aaccggagga acgggcggtg gaatcaacat cagcgttcca gccgcaaata cacagagttc
      181 aatctcatct cagcaaaatt cagtaggaat taaaggaatt gcttttcctt cgctttctaa
      241 tgcagctcag ccttccggag ccacgaacct caatctgctt atggatgtgc agatgtcagt
      301 gactgtggaa ctgggaagaa caaaaatgta tatcaaggat attctgggac ttggcgaggg
      361 ttccattatc gagctggata aactggccgg tgagcctgtg gatcttcttg taaacgggaa
      421 actgattgca aagggagagg tcgtggtcat tgacgaaaac ttcggtgtaa gggtaacgga
      481 tatcgtcagt cctacagaca gaatcaaatc cgaaggaaag taatgcccct tcggaaaatc
      541 ctcctcagtt tcgggctgat tcttacactg tccgtttctg aggtaaattc tcagtccaga
      601 acagaagaac tcgacaagga acttcggaag gaactcggtc aggaaaaaaa agaaaaacag
      661 acttccgaaa aaaacgagaa tactgaaaaa gaaaaatcag aaaaagaaac aaaaacctct
      721 gataaaaaac cggacaaaaa agaatctcca gaaaaaaatc cagtcctgga acgttacgac
      781 agttccaaag aagatgcccc cagcctttta tggattcttc ttaaagtatt ttttgtactt
      841 gccgtacttg tcggtgtttt ttattatctg ctccgcttaa tatccagaaa cagaaacctt
      901 aagtttccag ttcagggagc aatgcgtgtg ctcagcacac ttcctctcgg tccaaacaag
      961 gaacttcagc ttgtggaagt ttccggaatg cttgtgcttg tcggagtgac ggacaattcc
     1021 atcagcctga tcaaggaaat agactctcct gaaattaaac agaagatatt ttccattaag
     1081 gaatcatttg aacctcccaa ggaaaatttc ctggaactga tcgttcagac ggtcaaggaa
     1141 gacattcagt cagtcagcca taaaaagcag aaaattcaga tcgacgatga gcatgaagag
     1201 attctggacg aactgaagtc cagacagctg gacagactgg aaaaactgaa aaaagaaaga
     1261 cagtccattt ctaaaaagaa agactgaaaa catatca
//
LOCUS       DAOQGJ010001463         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84119_length_1297_cov_2.488728, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001463 DAOQGJ010000000
VERSION     DAOQGJ010001463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84119_length_1297_cov_2.488728"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PK683_18770"
     CDS             <1..304
                     /locus_tag="PK683_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10553.1"
                     /translation="GSSIPAASRLGTPGIVHNGFILLKNYRKSDFASPDLELSHIQAD
                     DLVFEGTIETRTEGIGNFTAHIFRHISPNQKVKIASVRQEIRETLAVLEGGSHKNF"
     gene            447..>1297
                     /locus_tag="PK683_18775"
     CDS             447..>1297
                     /locus_tag="PK683_18775"
                     /inference="COORDINATES: protein motif:HMM:NF016593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNH10554.1"
                     /translation="MRLPKINLPRKPVYVKLDSSIDFFSLFQTIEKECETCFFLESLE
                     RESYHSRFSIIGFEPESVLSAKTGVLIFNDREYEAQNPYHLLREIMPEPILGRDYSGG
                     LIGYISYEAMNLFEPVLNLPTDSRFPLFQFGVYKDGLIRNKVTGEIMYFYFENSRLDL
                     IQKWIKNTADQSSSLKLKAYGYTMSQSQHKEGVLSILEEIRSGNTFQCQLGFKSEFEL
                     KGSPIPIYKKLREINPSPHMYCMKFKNVTVIGASPELLFRLANGEMETFPLAGSIRRG
                     KDDEEDIL"
BASE COUNT          379 a          281 c          266 g          371 t
ORIGIN      
        1 cggaagctcc attcctgcag cgtctaggct tgggactcct ggaattgtcc acaacgggtt
       61 tattctgctg aaaaactaca gaaaatcgga ctttgccagc cctgatctgg aactttctca
      121 tattcaggcg gatgatcttg tatttgaagg cacaattgaa acaaggacag aaggaatcgg
      181 aaatttcaca gcacatattt tccgtcacat ttcaccgaac caaaaggtaa aaatagcatc
      241 tgtccgtcaa gaaatccgtg aaacactggc cgtgcttgaa ggaggcagtc acaaaaattt
      301 ctgaactttt tttttgaaat aagtctaagt attcagatca tcgtcgtttt ttgtatccaa
      361 catttccctc tcacttccgg ggagtttccc tccccttttt ttttattttt ctttaccgta
      421 accccttctc caaaaacaac aaatccatga gacttcccaa gattaatctt ccgaggaaac
      481 ctgtttacgt taagctggac agcagtattg attttttcag ccttttccag acaattgaaa
      541 aggaatgcga gacctgtttt ttcctggaat cactcgaaag agagagttat cactccagat
      601 tttccatcat cggatttgag cctgaatcag ttctgtctgc aaaaaccggg gttctcattt
      661 ttaatgacag ggaatatgaa gcacagaacc cataccacct tctcagggaa attatgcctg
      721 aaccgatttt gggcagagac tattcaggcg gtctgatcgg gtatatcagt tatgaggcga
      781 tgaacctttt tgaacctgta ctgaatcttc cgactgattc cagatttcct cttttccagt
      841 tcggcgtata caaggacgga ctgatcagga acaaggtgac aggcgaaatt atgtatttct
      901 attttgaaaa cagcagactg gatctgattc agaaatggat taagaatacc gcagaccagt
      961 cttcttcact gaaactcaaa gcatacggtt atacaatgtc gcagtctcag cacaaagagg
     1021 gagtgctttc cattctagag gaaatcaggt caggaaatac atttcagtgc cagcttggat
     1081 tcaagtctga gtttgaactg aaaggctccc ctattcctat ttataaaaaa ctgcgggaga
     1141 tcaatccgtc tcctcatatg tactgtatga aatttaaaaa tgtgactgta atcggtgcaa
     1201 gccctgagct actcttcagg ctggcaaacg gggaaatgga aacctttcct cttgcaggat
     1261 ccatcagaag aggaaaggat gatgaggaag atattct
//
LOCUS       DAOQGJ010001464         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84232_length_1296_cov_2.660757, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001464 DAOQGJ010000000
VERSION     DAOQGJ010001464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84232_length_1296_cov_2.660757"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1033
                     /locus_tag="PK683_18780"
     CDS             344..1033
                     /locus_tag="PK683_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10555.1"
                     /translation="MLSNCSIFQFSRYSDFADWYIGKAGFYQTLQVPDLKKGRCFGDN
                     EDYNYVIIAEDRHPIFLHFRTPEIYWNPQGWMYYPSTFNYCGILKRGRIPSKYKKVPL
                     GRSAESRSLNYLGGEAGLLDSLIPYKVLTAENMDMDSKMQFVYFSPILFFFNFLGKLF
                     VFPVYMFHDIIKIPVIPAAVIYYSIPSEKEPVNEIQKDGTEKDNKNKDKYKGLLPEEA
                     EEMRIREGEKN"
     gene            1114..>1296
                     /locus_tag="PK683_18785"
     CDS             1114..>1296
                     /locus_tag="PK683_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10556.1"
                     /translation="MLREMRLAVISFFLCWPCFAETQAEIREKEKNSVIMFFDNTGKG
                     IGFQQNLNKDFFAGIYY"
BASE COUNT          417 a          200 c          282 g          397 t
ORIGIN      
        1 aatgcaggca tattggtatt ctgagcaaaa gtgtcagaaa ccatgagaca gaatattttg
       61 tattttaccg ttcttatttt ggttggtgta ttttcgggaa actgttatga aatgtacact
      121 gagagagttc gtatgcataa ggaaagctgt aaagctcaaa ggagagaaaa ttttaaaatt
      181 agaagtattg gctgcattca aaaacttcaa agcgataata ccgcaagcaa agaggataaa
      241 tcttttgatg aaaatttttg tctggtcata ctggcttcta tttcagcaaa ttgtcgttaa
      301 tgggaaaaat gctgttttca ttattttctg catttttgaa ttaatgttgt ccaactgttc
      361 gatttttcag ttttccagat actctgattt cgcagactgg tacatcggaa aggctggttt
      421 ttatcagact ctgcaggttc ctgatctgaa gaaaggcaga tgtttcggtg acaatgagga
      481 ctataattat gtcatcattg cggaagaccg gcaccctatt ttcctgcatt ttcgtacacc
      541 ggaaatttac tggaatcctc agggatggat gtactatcct tctaccttta actattgcgg
      601 catactgaaa cgaggaagaa tcccttccaa gtataaaaag gttcctctcg gaaggagtgc
      661 agaatccaga tcactcaatt atcttggagg agaggctgga ctgctagact ccctgattcc
      721 ctataaggta ctgactgcag aaaatatgga tatggacagt aaaatgcagt tcgtgtattt
      781 cagcccgatt ctttttttct tcaatttctt aggaaagctg tttgtgtttc cagtatatat
      841 gtttcatgat attataaaga ttcctgtcat tcctgcggca gtcatatatt attccattcc
      901 ttcggaaaaa gagccggtaa acgaaataca gaaagacggt acagagaaag ataataaaaa
      961 caaagataag tataagggac tgctgcctga agaagcagag gaaatgagaa tcagggaagg
     1021 agaaaaaaat tgaagtacga tgtaaataga aaaacggttc tgatttactt atgcaggaag
     1081 ggtcttaact caaaaagatg aagggagatg actatgttaa gggaaatgcg tttggcagtg
     1141 atttcttttt tcttatgctg gccgtgcttt gctgaaacgc aggcagaaat cagagaaaaa
     1201 gaaaagaatt ctgtaattat gttttttgac aatacaggta aaggaatagg gtttcagcaa
     1261 aatctgaata aagatttttt tgcaggtata tattac
//
LOCUS       DAOQGJ010001465         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84322_length_1295_cov_3.287903, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001465 DAOQGJ010000000
VERSION     DAOQGJ010001465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84322_length_1295_cov_3.287903"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PK683_18790"
     CDS             complement(<1..1161)
                     /locus_tag="PK683_18790"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNH10557.1"
                     /translation="MLKYGKMTTDSVRQIFFLLISAMLIHVGLYAEDRVLNLGTVGNA
                     SVSLTEYISVLEDPGRSLTLNDVSSGKNSESFRTVKSSGGALNFSFSSSAFWLRFKIK
                     NTDAFPRELKAVISYPRLEYIDFFVPDRNGYRTVRSGYSVPHDERPEKSRFFVFPLTL
                     PAEAEQVFYFRISSQNSVNIPVSLWDKNAYYKYELNDHVIQALYFGIVISMALFNLLI
                     YTILRDINYILYVTFIILTGFTIASYNGIASELFWNHSPWLDRVAVNALISSVLVLFL
                     LFMRRMLSTKTVIPRLDKVNKFLIAGQVVICILYLISFSIFVKVIVISHALTGFWILC
                     VGGVCAYRRQRSAYFFVMAFGVLFIAIILGTLRALGLLPVNPFTVEGPQFGSA"
BASE COUNT          407 a          268 c          278 g          342 t
ORIGIN      
        1 ggctgaaccg aactgaggac cttccactgt aaaaggattt accggcagga gtccgagtgc
       61 cctgagcgtt cccaggataa ttgcaataaa aagtacaccg aaagccatta caaaaaaata
      121 tgcgcttctc tgtcttctgt atgcacagac tccgccgacg cagaggatcc agaatcctgt
      181 caatgcatgg ctgatgacaa ttacttttac aaatatgctg aaggagatca gatagagaat
      241 gcagatgacg acctgtcctg ctataaggaa tttattgact ttatcgagac ggggaatgac
      301 tgtctttgtt gagagcattc tccgcataaa gagcagaaat aatacaagaa ccgaagagat
      361 cagtgcattt actgcgactc tgtccagcca gggggaatgg ttccagaaca gttcggatgc
      421 aatcccgttg tagcttgcta tcgtaaatcc cgtcaatatg ataaatgtta catataatat
      481 ataatttata tctctaagaa tcgtataaat cagaagattg aaaagagcca tagagatgac
      541 tatgccgaaa tagagtgcct gaatcacatg gtcgttcagt tcatatttat aataagcatt
      601 cttatcccat aagctgacag gaatattgac ggaattctgt gaactgatcc tgaaataaaa
      661 tacctgttca gcttctgcag gaagagtcag agggaaaaca aaaaatcggc ttttttccgg
      721 acgttcatca tgagggacag aataccctga acggactgtc cgatatccgt tcctgtccgg
      781 gacgaaaaaa tcaatatatt caagtcgcgg gtaggaaatg acagccttga gttctctggg
      841 gaatgcatcc gtatttttta tcttgaatct gagccagaaa gcagaagagg aaaatgaaaa
      901 attcaatgct cctcctgaag atttcactgt ccggaaagac tctgagtttt ttcccgagga
      961 aacatcattc aaggtcagtg aacgccccgg gtcttcaaga acggaaatat attcagtcaa
     1021 agaaacggat gcatttccga ctgttccaag attaagcact ctgtcttcag catacaagcc
     1081 gacgtgaatc agcattgcgg agatcagcag gaaaaaaatc tgccttactg aatcagttgt
     1141 catctttccg tatttcagca tactcaattt catctcgttc agcaggaagt gccaggataa
     1201 tttctgtatg gacgatgatc tgcagtctca gcatcactgc aattcttttt gcttccaaaa
     1261 gtgaaattct gaacttgtgc agagctcttc tgaaa
//
LOCUS       DAOQGJ010001466         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84361_length_1295_cov_2.389516, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001466 DAOQGJ010000000
VERSION     DAOQGJ010001466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84361_length_1295_cov_2.389516"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PK683_18795"
     CDS             <1..1084
                     /locus_tag="PK683_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10558.1"
                     /translation="HSGGVYALAQADIQANIGIWLVTKVIDANTFQIVQSGYVTGLSG
                     GTADETWYLSDTTAGGLVNVAPTIAQPIIYWITASEGWVLGYPATSSGVIPSGAAPNG
                     YNNLSVTFTGNTVTYEGDTVVQGQSLGLNNSVIVSGAAGWRYGVIDTLGNASHEVIPP
                     AEIITDNQQYTPNPPFDSAWNGYYSSVNPTKRICWILYFDGTNIIEVISYGNGKDKND
                     DWYHAAGNTSPASTLNERLQYVTAFSRTRGTNISAVDNGFGTTDNEGFRITSLSDGYM
                     IIDFGITISCPAGVNSVISCNKNGAAWMGNIAQLYCPPGMTIATTTRVHIECQVTSGD
                     FFTFYVSVGATNSYFDGMEIKFRSHV"
     gene            1084..>1295
                     /locus_tag="PK683_18800"
     CDS             1084..>1295
                     /locus_tag="PK683_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10559.1"
                     /translation="MYYYNNSGKLQTSPIPIKGKTAVPLTADQAKICIGFDSQGNPIF
                     DNSILEKEQARAKEKAVYEIKTAFENA"
BASE COUNT          389 a          260 c          301 g          345 t
ORIGIN      
        1 gcattcaggc ggggtttatg cacttgcaca ggcagacatt caggcaaaca taggaatctg
       61 gcttgtcaca aaggttattg atgcaaacac tttccagatt gttcagtctg ggtatgtaac
      121 cgggctttcc ggcggaacag ctgacgaaac ctggtatctg agtgacacta ctgcaggcgg
      181 gcttgtcaat gtcgctccta caattgcaca gcctattatt tattggatca cagcaagcga
      241 gggatgggta ttaggttatc ctgccacttc ttcaggtgtg attccttccg gtgcagctcc
      301 gaacggatac aataatctta gcgtgacatt caccggcaat actgtcactt atgaaggtga
      361 tacagttgtc caaggtcagt ctctcggtct gaataacagc gtaattgtaa gcggtgcagc
      421 aggctggagg tatggagtaa ttgacacttt aggcaatgct tcgcatgaag taatcccgcc
      481 tgctgaaatc ataactgata atcagcagta tacacctaat ccgccttttg attcagcttg
      541 gaacggatat tattcaagcg tgaaccctac aaaaagaatt tgctggattc tgtatttcga
      601 tgggacaaat atcattgaag ttatttctta cggaaacggg aaagacaaga atgatgactg
      661 gtatcatgcc gctgggaata caagccctgc gtcaacactg aacgaaagac ttcagtatgt
      721 cactgctttc agcagaacaa gagggacaaa tatttcagca gttgataatg gattcggcac
      781 taccgataac gaggggttca gaattacgtc tttgtcagac ggatatatga taattgattt
      841 cgggattact ataagctgtc ctgcaggcgt aaactcagtt atttcctgca ataagaacgg
      901 ggcagcgtgg atgggaaata ttgctcaatt atactgcccg cccggtatga caatagcgac
      961 gacaacaaga gttcacattg aatgtcaagt tacatcgggg gatttcttta ccttctatgt
     1021 ttctgttggt gcaacaaatt catatttcga cggaatggaa attaaattca ggagtcatgt
     1081 ataatgtatt actacaataa cagcgggaag cttcagacaa gtccgatccc tataaaaggg
     1141 aaaacagcag tgcccctgac agcagatcag gccaaaatct gcattggttt tgattcacag
     1201 gggaatccga tctttgacaa ttccattctt gaaaaagaac aggcaagagc aaaagaaaaa
     1261 gctgtttatg aaataaaaac tgcttttgaa aatgc
//
LOCUS       DAOQGJ010001467         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84362_length_1295_cov_2.381452, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001467 DAOQGJ010000000
VERSION     DAOQGJ010001467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84362_length_1295_cov_2.381452"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>1295
                     /locus_tag="PK683_18805"
     CDS             59..>1295
                     /locus_tag="PK683_18805"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNH10560.1"
                     /translation="MIKFLKKNILSGKINYNLSKIPVSCEIAEGLKKIRFSDETEYEF
                     DDIFLCTSGRPPELAGASDLSRNESGFFQVNSFLQTLSHSNVFASGDCASMQGLSVPK
                     AGVYAVRQGKPLSENIIRFTEGKPLKEYIPQKSALALVYSGNSRAFAVKGNFFVPPSS
                     LLFSWKDGIDRKFMNRFSEMEKMPAMKAADESMNEMHCAGCGSKISGKSLKNVIERIK
                     KETEKKSEDSDFIGEIAKNISDFFSETKSRRSELILDMHSSEDVSSVLPPKKMRLLQS
                     TDHFSPMISDHHTYSRILTLHALSDLYAKGAVPDSVLISLTLPYMPDGAAEETAYQLL
                     SGAVGLLSSLGIHVLGGHTSEGPELQMGMTCNGFVYPGRERLKKNLKSGDALILTKPL
                     GTGLIFAADQRCLAEGRWVE"
BASE COUNT          381 a          281 c          303 g          330 t
ORIGIN      
        1 ggaggcagag attcagattt ttgaaaaatc ggatacgttg ccgaagttca atcccggaat
       61 gataaagttc ctaaaaaaaa atattttgtc tggaaaaatt aattataatt tgagcaagat
      121 tccggtctcc tgcgaaatcg ctgaaggtct gaaaaaaatc cggttttctg atgaaactga
      181 atatgaattt gacgatatat ttctgtgcac ctcaggccga ccgcctgaat tagcaggcgc
      241 atcagatctc tcccgcaacg aatcgggctt ctttcaagta aattcctttc tgcagactct
      301 ctcgcattcc aatgtgtttg catcgggaga ctgcgcatcg atgcagggac tctccgttcc
      361 caaggcaggg gtatatgcag tcagacaggg aaaacctctt tccgaaaata tcatccgttt
      421 tacagaagga aaacctctga aagaatacat tccgcagaaa tccgcactgg cacttgttta
      481 ctctggaaac agcagagcat ttgcagtgaa agggaatttt ttcgttcccc cctcttctct
      541 tcttttttcc tggaaggacg gaatagacag aaaatttatg aaccgattct cggaaatgga
      601 gaaaatgcct gcgatgaaag ccgccgatga atcgatgaac gagatgcact gtgcaggatg
      661 cggatcaaag atttcaggaa agtctctgaa aaatgtaatt gagcggatta aaaaagagac
      721 agaaaaaaaa tcagaagatt ctgatttcat cggggaaatt gcgaaaaata tttcagattt
      781 tttctcagag acgaagagcc gacggtctga actgatcctg gatatgcact cctccgagga
      841 tgtttcttca gtacttcctc caaagaagat gagactgctt cagtccactg atcatttctc
      901 tcctatgatc agtgaccatc atacatacag cagaatcctg actcttcatg cactgagcga
      961 tctgtatgca aagggggcgg taccggacag cgttctgatc agccttaccc ttccttatat
     1021 gccggacgga gcagcagaag aaacagcata tcagcttctt tccggggcag tcggactgct
     1081 gtcctcactc ggtatccatg ttcttggagg tcacacttcg gaaggaccgg aacttcagat
     1141 gggaatgacc tgcaacggat ttgtctatcc gggcagagaa agactcaaaa aaaatctgaa
     1201 atccggagat gcgctgatcc tgaccaaacc tttgggaaca ggactgatct ttgcggcaga
     1261 tcagagatgt ttggctgagg gcagatgggt tgaag
//
LOCUS       DAOQGJ010001468         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84485_length_1294_cov_2.366425, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001468 DAOQGJ010000000
VERSION     DAOQGJ010001468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84485_length_1294_cov_2.366425"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PK683_18810"
     CDS             <1..243
                     /locus_tag="PK683_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNH10561.1"
                     /translation="LLEAMVENKICIASDIPVFKEILDSSDILVPPRDIEAWKKAFKI
                     FASKKDKTREKKVTDSDWTWKAAAVQIEESLKKVLQ"
     gene            240..>1294
                     /locus_tag="PK683_18815"
     CDS             240..>1294
                     /locus_tag="PK683_18815"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002179700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNH10562.1"
                     /translation="MKFNTLEFLIFFPISLIIGNLLKGTAQRIFLLLASYYFYMAWNK
                     TFIILILISTLLDYAVSLWLEKIPDSEKTKRKLVLTVSMVGNLGFLAFFKYTNFLLEV
                     FQDTGLFSMQSWGAYVSPVGISCGWNSGAAKYICDITLPVGISFYTFQSMSYTIDVYF
                     RKIEARKSFIDFSLYVAFFPQLVAGPIVRATTFLRDLDMRLSVTHEDIQLAFSRILIG
                     FMRKVVFADNLAKVVDMTFANYGSMSPIEIWTGVIAFGWQIYFDFAGYTDIAIGVARL
                     FGFQFDPNFNFPMSTNSIMDHWTKWHISFSTWIRDYIFIPLGGSRSGELRTYRNLFIT
                     WFFAGVWHGAAYHFISWG"
BASE COUNT          367 a          272 c          270 g          385 t
ORIGIN      
        1 cttctggaag caatggtcga aaataaaatc tgcattgctt ccgacatccc cgttttcaag
       61 gaaatactgg acagctccga cattcttgtc cctccgagag atattgaggc ttggaaaaaa
      121 gcttttaaaa tttttgcttc taaaaaagat aaaacaagag aaaaaaaagt gactgattct
      181 gattggacat ggaaagcagc tgcagttcag attgaagaaa gtctcaaaaa agtcctgcaa
      241 tgaaatttaa tacactagaa ttcctgattt ttttccccat ttccctcatc attggaaacc
      301 tgctgaaggg aacagcacag agaatattcc ttcttctggc cagttactat ttttatatgg
      361 cctggaacaa aacattcatc atactgatcc tgatctccac ccttcttgac tatgcagtct
      421 cactctggct tgagaaaatt ccagactcgg aaaagaccaa aagaaaactt gtgctgacag
      481 tctctatggt gggcaacctg ggattccttg catttttcaa atacacaaac tttcttctgg
      541 aagtatttca ggacacaggg ctgttctcaa tgcagagctg gggggcctat gtttctccgg
      601 taggaatctc ctgcggatgg aattcaggtg cagcaaaata tatctgtgat attaccctgc
      661 cagtagggat ttccttttat acttttcagt ccatgagcta tacaattgat gtgtatttca
      721 gaaagattga agcaagaaaa tccttcattg acttctctct gtatgttgca ttctttcctc
      781 agcttgtagc aggaccgatt gtaagggcaa ctacatttct gcgtgatctg gatatgaggc
      841 tttcagtcac tcatgaagac attcagcttg cattttcgag aattctgatt gggtttatga
      901 gaaaagtcgt atttgcagac aacctggcca aagtggtgga tatgactttc gcaaattacg
      961 gcagcatgtc gcctattgaa atctggacag gcgtcattgc gttcggctgg cagatctact
     1021 ttgacttcgc aggatacact gacatcgcca taggtgtggc aagacttttc ggatttcagt
     1081 ttgatccgaa tttcaatttc ccgatgagca caaacagcat tatggaccat tggacaaaat
     1141 ggcatatttc attttccaca tggatcagag actatatctt tattcctctc ggaggatcca
     1201 gatccggtga actgagaaca tacagaaacc ttttcatcac ctggtttttt gcaggagtct
     1261 ggcatggagc agcataccat ttcatcagct gggg
//
LOCUS       DAOQGJ010001469         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84550_length_1293_cov_3.619548, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001469 DAOQGJ010000000
VERSION     DAOQGJ010001469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84550_length_1293_cov_3.619548"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..>1293
                     /locus_tag="PK683_18820"
     CDS             171..>1293
                     /locus_tag="PK683_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002723003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNH10563.1"
                     /translation="MKIFRRLLRYSFHYKGRLITGILLSFLVSIFNGASLTSMVPIFD
                     SMGTSKDYKFRISYTKRDLDLFGKQSANEKLSRIESIELYGAVLKKRINDYFENKTPD
                     EVAVTFVILVFPVYILKLICLTGAIFFINSAGALATRDLRFELFAKSQQLSMDMFVKE
                     KTGILMSRIINDVDALGKVISSDLKDAIIDFFYILTHLVILFLLSWQLFFLTFVVIPL
                     IMGPISGFAEKIRKATKNQQERLSALNGHLQEVIAGIRVIRAFNTEKRESDKFFHIND
                     ELSMKTFKGHFYHQVGPSLVELFGAVIAAIFLAFGAYLISTQNFSKGMFLAFFLTLVF
                     IMRPLKQLSVMYNLIQSAVSAGERVFEIIDKEPDLTSREN"
BASE COUNT          383 a          259 c          277 g          374 t
ORIGIN      
        1 aatggaagca ggcggccctg actggctcag ttcctccaaa agagagatca ttgcaaaatt
       61 caaggctgaa aaagacagga tcaaactgga gatggagaac gagggaaaat aatcagtcgg
      121 agaaatcttc tttactcagg caggcattaa aaaaacgctt cttgtaacct atgaaaatat
      181 tcagaagact gctcaggtat tcattccatt acaagggcag actgattaca ggaatacttc
      241 tttcttttct ggtttccata ttcaatgggg cctctctgac aagcatggtt cccatttttg
      301 attccatggg aacctccaag gattacaaat tcagaatctc ctacacaaaa agagatctgg
      361 accttttcgg gaagcagtca gcaaatgaaa aactttccag aattgaaagc atagaactgt
      421 acggtgctgt cttaaaaaaa agaatcaatg attattttga aaacaagact cccgatgagg
      481 tggcggtcac ttttgtaatc cttgtattcc cggtctatat tctgaagctc atctgtctta
      541 caggggcaat atttttcatt aattctgcag gggcactggc aacccgtgat ttgaggtttg
      601 aactctttgc taagtctcag cagctttcca tggatatgtt tgtaaaggaa aaaacaggga
      661 ttctgatgag caggatcatc aatgatgtgg atgcacttgg aaaagtcata tccagcgatc
      721 tgaaagatgc catcatagat tttttctata tccttaccca tcttgtcatt ttatttctgc
      781 tcagctggca gcttttcttt ctgactttcg tagtcattcc tctgattatg ggaccgatca
      841 gcggatttgc cgaaaaaata aggaaggcaa caaaaaacca gcaggaaaga ctctctgcac
      901 tgaacggcca tcttcaggag gtcattgcag gaatccgagt gatccgtgca tttaatacgg
      961 aaaaaagaga atcggacaag ttttttcata tcaatgacga actttccatg aaaactttca
     1021 aagggcattt ttatcatcaa gtaggaccgt cccttgtgga gcttttcggt gcagtgattg
     1081 cagcgatttt tctggcattc ggagcctatc tgatttccac acagaatttt tcaaagggaa
     1141 tgtttctcgc tttttttctt acgctggtgt ttattatgcg cccattaaag cagctgagcg
     1201 tgatgtacaa tctgattcag agtgctgtgt ctgcagggga aagagttttt gaaatcatcg
     1261 acaaggaacc ggatcttacc tccagagaaa acc
//
LOCUS       DAOQGJ010001470         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84654_length_1292_cov_3.388036, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001470 DAOQGJ010000000
VERSION     DAOQGJ010001470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84654_length_1292_cov_3.388036"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..560
                     /locus_tag="PK683_18825"
     CDS             162..560
                     /locus_tag="PK683_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNH10564.1"
                     /translation="MEKTIKDILNTGIGLLQAGKEGLDKTRVDLEKIYSEISSKGALD
                     NSENAVKLRESLDKILAEIKEFSSVAGKNYEETRNKIIDNYNKIVEEVKTKMPEGKIE
                     EIKAKIDEIAASIKNATSGGNASGGTSAKA"
     gene            632..>1292
                     /locus_tag="PK683_18830"
     CDS             632..>1292
                     /locus_tag="PK683_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNH10565.1"
                     /translation="MKKILVTTALPYANGSIHLGHLLEGIQADIFVRYQRLSGNRCYF
                     FCADDTHGTPVMLAARKEGISPEDMIGRIHTEHYKDLSAFHISYDNYYTTNSPENRYF
                     SETIYLSLKKEGNIEEKEIEQLYCEHDRMFLPDRFIKGECPKCASKDQYGDGCEVCGS
                     AYSPADLKSPACAICGRPPVPQKSKHLFLHFRNLNEYLQEWINNPLHVHSGVRNKLQE
                     WF"
BASE COUNT          437 a          249 c          262 g          344 t
ORIGIN      
        1 gggaaaaaaa gggaaattta tccgtctagt ctgctgatta ttctttttta ttttcctaag
       61 tatctttgac tatcctgaac ttcggcaaaa taaaaaaaat gacaaatgag caaaatccag
      121 aaaaatggtt attaagatac acatctatac ggagagtaac gatggaaaaa acaatcaaag
      181 atatcctgaa tacaggaatc gggcttcttc aggctggaaa agagggattg gacaagacta
      241 gagtagatct ggaaaaaatt tattctgaaa tttcttcaaa aggcgctctt gacaattcag
      301 aaaacgctgt aaaactgaga gagtctcttg acaagattct tgctgaaatc aaagaattca
      361 gcagtgttgc cggcaaaaac tatgaagaaa ccagaaacaa gattatcgac aactacaata
      421 aaatagttga agaagtgaag actaaaatgc cggaaggcaa gattgaagag atcaaagcta
      481 agatcgatga gatcgctgca agcatcaaaa atgctacttc cggcggaaac gcttccggcg
      541 gaacttcagc aaaagcttaa tcctgtttat ctgagaactg cgaaagtttt ctttcgcagt
      601 ttttttttct tctgacaaaa aataaaaacc aatgaaaaaa atattagtca ctacagctct
      661 accttacgca aacggatcca ttcatttagg acatcttctc gaaggaattc aggcagatat
      721 atttgtgcgg taccagaggc tttccggaaa cagatgttat tttttctgtg ccgacgacac
      781 ccacggaacg cctgtcatgc tcgcagccag aaaggaaggc atttctcctg aggatatgat
      841 tggaagaatt catacagaac attataaaga tctttctgca tttcatatca gttatgataa
      901 ttattatact acgaactcgc ctgaaaacag atatttctct gaaaccattt atctctctct
      961 gaaaaaagaa ggaaatatcg aagagaagga aatcgagcag ctttactgcg aacacgacag
     1021 aatgtttctt cctgacagat ttattaaggg ggaatgtccc aaatgtgctt cgaaggatca
     1081 gtatggagac ggttgtgagg tctgcggttc agcctattca ccggctgatc tgaaaagtcc
     1141 tgcctgtgcc atctgcggga gacctcctgt tccccagaaa tccaagcatc tctttctgca
     1201 ttttcgaaat ctgaacgagt atcttcagga atggataaat aatcctcttc atgttcattc
     1261 gggagtccgc aacaagcttc aggaatggtt cc
//
LOCUS       DAOQGJ010001471         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84659_length_1292_cov_3.223929, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001471 DAOQGJ010000000
VERSION     DAOQGJ010001471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84659_length_1292_cov_3.223929"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK683_18835"
     CDS             <1..228
                     /locus_tag="PK683_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10566.1"
                     /translation="FSQGGFFVRVDVLIKKDNHVELIEVKAKSYNPSEDEFFDKRLLK
                     KGVHSSFYPKKDKLWKRMESVISKLRQRFQF"
     gene            complement(231..1283)
                     /gene="lpxD"
                     /locus_tag="PK683_18840"
     CDS             complement(231..1283)
                     /gene="lpxD"
                     /locus_tag="PK683_18840"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNH10567.1"
                     /translation="MREFNLREIGGLLAGSRIINSAEPEKTVFHSLGSLESGKGKFLS
                     FAAGKGNLKSAKASGAAALIISPDLEKEISVPAAVADNVELALSMVLSYMYPPKKPEG
                     KIESTSAVHSSAKIGKGTYLGHFVSVGENSTVGENCIIEDGVKIGMNVHIGDNARIGM
                     NCVFHDDTVIGKNFTVFGNSTFGGDGFRFVTVKGIHHKIPQIGKVVVGDDVEIGSNCC
                     VDRGGLEDTVIGNGCKFDNQVHVGHNVRIGNNVIIAGQSGVAGSTVIGNNVLIGGACA
                     VSDHLVVPDDSIIAGGTGMRTSPKEKGIYIGWDWGMTYPEFQKARVNIKHLIGFNKWA
                     SRIKAIEKQLGIKAEE"
BASE COUNT          356 a          331 c          219 g          386 t
ORIGIN      
        1 ttttctcagg gcggtttttt tgttcgagtt gacgtactta ttaaaaaaga caatcacgtc
       61 gaactaattg aggtcaaagc gaaatcctac aacccttcgg aggatgaatt ttttgacaaa
      121 cggctcctga aaaaaggagt ccattcttca ttttatccta aaaaggataa actctggaaa
      181 agaatggaat cagtgatttc caaactccgc cagagatttc agttttaact ttattcctca
      241 gcttttatcc caagctgttt ctcaatagcc ttaattctgg atgcccattt attgaagcct
      301 atcaaatgtt taatattgac tcttgctttc tgaaactcag gataagtcat tccccaatcc
      361 cagccgatgt aaattccttt ttctttcggt gaagttctca tccctgttcc gcccgcaatg
      421 atgctgtcat caggcacgac taagtgatcc gatactgcac aggccccgcc tatgagtaca
      481 ttgtttccta tgacagtgct tcctgcaaca ccgctctgac ctgcaatgat tacgttattt
      541 ccgatcctta cgttgtggcc gacatgcacc tgattgtcaa acttgcaccc attcccaatt
      601 actgtatcct caagcccccc tctgtccaca cagcagttgg aacctatctc cacatcatcc
      661 ccaacgacaa cttttccaat ctgaggtatt ttatgatgga ttcctttgac tgtgacaaaa
      721 cggaatccgt ctcctccaaa tgtagaattt ccgaatacag taaaattttt cccgatgaca
      781 gtatcatcat gaaatacaca gttcattcct attctggcat tgtctccgat atgaacattc
      841 atcccaattt taacaccgtc ttcgataatg cagttttcac ctacagtcga attctcacct
      901 acagacacaa aatgtcccaa atatgtgccc ttgcctattt ttgctgagga atggacagcc
      961 gaagtggatt caattttacc ctcaggcttc ttgggaggat acatatagct caataccata
     1021 gaaagagcaa gctctacatt gtcagcaact gcagcaggaa cggagatttc cttctccaga
     1081 tcaggactga taatcagtgc tgcggctccg gaagcctttg cagacttaag attccccttt
     1141 cctgcagcaa aggaaagaaa tttccctttc cctgattcca aggatccaag tgaatgaaaa
     1201 actgtttttt ccggctcagc agaatttata atcctgcttc cggccaataa cccgccgatt
     1261 tcccttaaat taaattctct caaagcagcc ct
//
LOCUS       DAOQGJ010001472         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84661_length_1292_cov_3.143897, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001472 DAOQGJ010000000
VERSION     DAOQGJ010001472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84661_length_1292_cov_3.143897"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PK683_18845"
     CDS             complement(<1..360)
                     /locus_tag="PK683_18845"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10568.1"
                     /translation="MIKKIRIQNFRNLKDVTVHLNEVNLFIGPNNSGKSNVLQAIRLA
                     SCILQRNLLERKETVYSDLVQDTEYDRQLDSIGLKAGENCILSFIIRFPENIDHEIFL
                     QILIRNSLPPSVEIYWGI"
     gene            527..787
                     /locus_tag="PK683_18850"
     CDS             527..787
                     /locus_tag="PK683_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10569.1"
                     /translation="MKSISVQFILILLISGSFFCRQRYSEEEMEKLQSRADCELCLRQ
                     DSMNACLINGKCKRLALYCQLRCQEKVFKGRDRCINSCWKDY"
     gene            complement(781..1185)
                     /locus_tag="PK683_18855"
     CDS             complement(781..1185)
                     /locus_tag="PK683_18855"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNH10570.1"
                     /translation="MEISEINKKISDIPFQKYSGIELVSFGPGWAKSKFTVTEEHLNA
                     IGVLHGGIINAMLDVACFLAVMTKLKENKFATTHTSTFTNLRPIKLGSVVEIHSELEG
                     IGKHTAFLKAEAFVNGKKSATASVCKSIIDRQ"
BASE COUNT          369 a          257 c          249 g          417 t
ORIGIN      
        1 tatcccccaa taaatttcga cagaaggagg cagtgagttt cgaatcagta tctgcaggaa
       61 aatctcatga tcaatatttt cgggaaatcg aataataaaa gaaagaatgc agttttcgcc
      121 tgcttttaaa ccgattgagt caagctgcct gtcatattca gtatcttgaa ccaaatcaga
      181 ataaactgtt tccttccgtt caagcaggtt tctttgtaaa atgcaagacg caaggcgaat
      241 ggcctgcaga acattgcttt taccggaatt attcggtcca atgaacaggt tgacttcatt
      301 caaatgaact gtaacatctt tcaaattccg aaaattctga attcgaattt tttttatcat
      361 tgtatacgac ttcctgtatt taatttcagc gaacggcata ggatttcaaa tcttttttca
      421 attgaaaatt gatttacttg attaattcct ttgcttcata gaaacactaa agcatcctga
      481 tacaaaattc ttttgcttat attcttgcag ttctgcctaa ttttatatga aaagcatatc
      541 agtgcagttt atcctgattc ttttgatcag tggaagcttt ttctgcagac agagatactc
      601 agaggaagaa atggaaaaac ttcagagcag ggctgactgc gaactctgtc tcaggcagga
      661 ttcgatgaat gcctgtctga tcaacgggaa atgcaaaaga cttgctcttt actgtcagct
      721 tagatgtcag gagaaagtct ttaagggcag agacagatgc ataaacagct gctggaaaga
      781 ctattgacgg tctatgatac tcttgcatac agaggctgtc gcagattttt ttccgtttac
      841 gaatgcctct gctttcagga atgcagtgtg ttttcctatt ccttccagtt ccgaatgaat
      901 ttccacaact gatccaagtt taatcggcct gagattcgta aatgtggatg tatgtgttgt
      961 cgcaaattta ttctctttca gtttagtcat cactgcaaga aagcaggcaa catccagcat
     1021 tgcgttgatg attcctccat gcagaacacc gattgcattc agatgttctt cggttactgt
     1081 gaattttgat ttcgcccagc ccggaccaaa actgaccagc tcaattccgg agtatttctg
     1141 aaaagggata tctgatattt tcttattgat ctcactgatt tccatagtgc atccttttta
     1201 ggacagtttt tcggaagtcc ttttttcata aagcattgca ccgcattgaa tatacttact
     1261 cacctgcgga tgtatggaca ggaacgcctg tc
//
LOCUS       DAOQGJ010001473         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_84799_length_1291_cov_2.995146, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001473 DAOQGJ010000000
VERSION     DAOQGJ010001473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84799_length_1291_cov_2.995146"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1291)
                     /locus_tag="PK683_18860"
     CDS             complement(<1..>1291)
                     /locus_tag="PK683_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNH10571.1"
                     /translation="EKEREKFLIATVKGDVHDIGKNIVAVVLACNNYDVIDLGVMTPC
                     ETILNEAEKLKVKAIGLSGLITPSLDEMCYVAKEMNARGMNIPLLIGGATTSPAHTAV
                     KIAPEYPNGTVTHVIDASRVVGVLSSLLSAENSAGFKKANEEAQEKLRLQYEKNKRDR
                     NLLSISDARKNRFQTDWDSYKPPVPEFTGIKIYDNVSLEDVSRFIDWSPFFHAWELRG
                     RYPDILEDETVGKEAKELFANAQTLLKKIISEKKIRLRAVIGFFPAFRKNDDILVFSR
                     KGDSEPAAVFRMLRQQMPKEEGEFNYSLSDYIAPEGYEDYIGGFTVTSGKEVDDFASE
                     FEKKGDDYNSILVKALGDRFAEALAEYIHREARILWGFGENENLSNEELIREKYRGIR
                     PAPGYPACPEHTEKRTLFRLLESEKNAGVTLTENCAM"
BASE COUNT          304 a          322 c          249 g          416 t
ORIGIN      
        1 ctcattgcac agttttccgt cagtgtcact cctgcatttt tctccgattc aagaagcctg
       61 aaaagtgtcc ttttttcagt atgttccggg caggcaggat atccgggcgc aggcctgatc
      121 cctctgtatt tttctctaat taattcttca ttagaaagat tttcgttttc cccaaacccc
      181 cagaggattc tggcttccct gtggatgtac tcagcaagtg cttctgcaaa cctgtctccc
      241 agcgctttta caagaatgga attgtagtcg tcgccttttt tctcaaattc agaagcaaag
      301 tcatcgactt cctttccgga agttacagtg aaccctccga tataatcttc atacccttcc
      361 ggtgcgatat aatcagatag actgtaatta aactcaccct cttctttcgg catctgctga
      421 cgaagcatcc tgaatactgc cgcaggttca ctgtctcctt tcctgctgaa gaccaaaata
      481 tcatcgttct ttctgaatgc aggaaaaaaa ccgatcacag ctctcagtcg gattttcttc
      541 tctgaaataa ttttcttcag aagagtctgt gcatttgcaa aaagttcttt tgcttccttt
      601 cctacagttt cgtcttcgag aatgtcagga taccgccctc tgagttccca ggcatgaaag
      661 aacggcgacc agtcaatgaa tctgctcaca tcttcgaggg atacattgtc atatatttta
      721 atgccggtaa attccggtac aggaggcttg taggaatccc agtccgtctg aaaacggttt
      781 tttcttgcat cggaaatgga aagaaggttt ctgtctctct tgtttttttc atactgcagc
      841 ctcagttttt cctgtgcttc ctcattcgct tttttgaaac ctgctgaatt ttctgcactt
      901 aaaagactgg aaagcactcc caccactctg gatgcatcaa tcacatgagt gactgttccg
      961 ttcggatatt caggagcaat cttcactgca gtgtgtgcag ggctggtagt tgcgcccccg
     1021 atcaggagag ggatattcat ccctcttgca ttcatttctt ttgcaacata acacatttcg
     1081 tcaagggaag gggtaatgag gccggaaagt cctattgctt tcaccttcag cttttctgct
     1141 tcattcagga ttgtctcgca gggagtcatt acacccagat caattacatc ataattattg
     1201 catgcgagca cgactgccac gatgttcttg cctatgtcgt gaacatctcc tttcactgta
     1261 gcgatcagaa atttttctct ctccttttca t
//
LOCUS       DAOQGJ010001474         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85039_length_1289_cov_2.912480, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001474 DAOQGJ010000000
VERSION     DAOQGJ010001474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85039_length_1289_cov_2.912480"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..>1289)
                     /locus_tag="PK683_18865"
     CDS             complement(35..>1289)
                     /locus_tag="PK683_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10572.1"
                     /translation="PIHPKLNDYSSFTSEGWSVNNDKLMYKLEKNSKIYYFMYSLKDR
                     TKTQIDLPGKFVSWTADDEMVISSEEGLFVKGKETRKLPVSNQNFDFYQIVRSSDGKK
                     LYGRYNQGIASIDLSSGSISSITPEGGYTEYQWPFPSPSGKRVAYEHQTGSDGGIPTG
                     TVDADGIPVIQYRGGSDFHWIGNKTIVIKDECSLRTADIETKKIKILKLDPKGRYIYQ
                     CNGNGQLPADEEITERETLRITSESGLFLREEPSEKSAQILLVPFAHTVFMNRRKKTD
                     ITVQGKTGRWTLVSYYSAGKEYEGWMFGGYLGPVRQDITAENIKGFKSGCFGSSCNGC
                     GSYDFSPDGKFRHSLSCHGGEITGKWALVGKEIRVCTKPKCSPKTDKELVFRASRDGQ
                     IIYENLGTAETVFNADQIYHWEPPH"
BASE COUNT          313 a          303 c          251 g          422 t
ORIGIN      
        1 gtcgaattat ggaaaagagt caattaaaat agtttcagtg aggcggttcc cagtgataga
       61 tctgatctgc attgaagact gtttctgccg ttcccaagtt ttcataaatg atctgtccgt
      121 ctcgcgatgc acggaagacc aattccttat cagttttagg actgcatttc ggttttgtgc
      181 atacacgtat ttcctttccg acgagtgccc attttcctgt gatctctcct ccgtgacagc
      241 tgagtgaatg gcggaatttt ccatccgggg agaaatcgta tgatccgcat ccattgcagg
      301 aagaaccgaa acatcctgac ttgaagcctt ttatgttttc agcagtaata tcctgacgga
      361 caggtcctaa gtatcctccg aacatccatc cctcatattc cttcccggca gaataatagg
      421 acacaagagt ccagcgtccc gtttttccct gtacggtaat gtctgttttt tttctccggt
      481 tcatgaatac agtatgagca aacggaacga gcagaatctg tgctgatttc tcactcggtt
      541 cttcacgcag aaagagtcct gattcagaag tgattctcaa agtttcccgt tcagtgattt
      601 cctcgtctgc cggaagctga ccgtttccat tgcactgata aatgtatctg ccctttggat
      661 ccagcttcag tatttttatc tttttggttt caatgtccgc tgtccttaag ctgcactcat
      721 ccttaattac aatcgtttta tttccaatcc aatggaaatc tgatccaccc cgatactgaa
      781 tgacaggaat tccatctgca tcgacagtgc ctgtcgggat gcctccatct gatcctgtct
      841 ggtgctcata agctactctt ttgcctgaag gggacgggaa aggccactgg tattctgtat
      901 aacctccctc cggcgtgatg ctgctgatac tgcctgaact cagatcaata cttgctatcc
      961 cctgattgta tcttccatac aatttttttc cgtccgagct tcttacaatc tgatagaagt
     1021 caaagttctg atttgaaacc ggaagttttc ttgtctcttt tcctttaaca aaaagacctt
     1081 cttcagatga tatgaccatt tcatcgtctg cagtccagga aacgaatttt ccgggaagat
     1141 cgatctgtgt ttttgttctg tctttcaggg aatacataaa gtaatatatt ttactgtttt
     1201 tttccagttt atacattaat ttatcattat tcaccgacca tccttccgaa gtaaatgaac
     1261 tgtaatcatt cagcttggga tgaatcggg
//
LOCUS       DAOQGJ010001475         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85192_length_1288_cov_2.725872, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001475 DAOQGJ010000000
VERSION     DAOQGJ010001475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85192_length_1288_cov_2.725872"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..185
                     /locus_tag="PK683_18870"
     CDS             57..185
                     /locus_tag="PK683_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10573.1"
                     /translation="MELIIRALPYLRPDFHPDQTDNRILSEEFFKAESEYFEKKAV"
     gene            complement(473..>1288)
                     /locus_tag="PK683_18875"
     CDS             complement(473..>1288)
                     /locus_tag="PK683_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10574.1"
                     /translation="AVNSIWPVAIVGGVGAKALKVVVEVVKVGVSGVVYLIAPNAKLA
                     LAGIVGSAKFIGGEAFNGADVSARKGANAALYTASKTVQATGIITEKTGTYVLAPAAL
                     GGAVIGQSLVGGGVAVGGSVVGGAVVGTTGTASAVVYTGTKAADYTVSGVGTGISGGV
                     AAGYGVYYSAKAVGVPAGVTVGSGVVLSYEAVAQLSAQTILAVSDVSYLVLSMEGGKW
                     VVYGIKDTTGKAKTLLSGAVVDVDQIRKEGGEVYKVPASDEEIERMLDKSKHK"
BASE COUNT          336 a          359 c          249 g          344 t
ORIGIN      
        1 atgcataaac atttaatcaa ttcttattta tactgctatt ccgcaacaga ctctttatgg
       61 aactgataat aagagcactt ccttaccttc gtcctgactt tcatcccgat cagactgata
      121 acagaattct ctcagaagag tttttcaaag ccgagtcgga gtatttcgaa aagaaagcag
      181 tctgaccttt tttctgactg agctcagttg aacggcggtg aaaattgtaa gctttgatat
      241 aaatcagcac aagtcgagac atcattctgc ataatatgtt tgaaagtcta tcgctaattg
      301 cagagcatga attaggcgag ccccatcaag tctgatttct gacagccctt tgggcagctg
      361 ctccgctgtt aaaaattcgg gtaaatgaat acagatgcct tgaagcctga acatctgtat
      421 tcaaccggtg tcatatcgac tgtacatcta tgcacatctg cacagtgagt atttacttat
      481 gcttggactt gtcgagcatc ctttctattt cttcgtcaga ggcaggaacc ttgtaaacct
      541 ctcctccctc tttcctgatc tgatcgacat ccactaccgc accggaaaga agagtcttgg
      601 ctttcccagt cgtatccttg attccgtaca cgacccactt acctccttcc atcgaaagga
      661 caagatagga cacatcgctg actgcaagga ttgtctgtgc ggagagctgt gccaccgctt
      721 catagctgag aaccacacct gatcccacag tcacgcctgc cggaacaccc actgcttttg
      781 cagaatagta tacaccgtat cccgcagcga cacctccgga aattccagtt ccgacacctg
      841 aaacagtata gtctgccgcc ttggttcctg tatagacaac agcacttgca gttccagtcg
      901 ttccgactac tgcaccgccg acaactgatc ctcctactgc aacacccccg ccgaccagac
      961 tctgtccaat gacagctcct cctagtgcag caggagcaag aacataggtt cctgtttttt
     1021 cagtaatgat tcctgtcgcc tgcacagttt tcgaagcagt ataaagtgca gcattcgcac
     1081 cttttctggc ggatacatcc gcaccgttga atgcctctcc tccgataaac tttgcacttc
     1141 ctacaatgcc ggccagtgca agttttgcgt tcggagcaat gagataaaca acaccggaaa
     1201 cccctacttt aacgacttcc actactacct tcagtgcctt ggcacctacc cctcctacaa
     1261 tggctacagg ccatatggaa ttgacggc
//
LOCUS       DAOQGJ010001476         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85227_length_1288_cov_2.106245, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001476 DAOQGJ010000000
VERSION     DAOQGJ010001476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85227_length_1288_cov_2.106245"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..748
                     /locus_tag="PK683_18880"
     CDS             266..748
                     /locus_tag="PK683_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH10575.1"
                     /translation="MNKINTEIQMIQDGHRDQAIILLNQFFSLVNSLPLDGIFEVRPN
                     AARKMIDTFLKLQGTDKVCFAGVFQESELLSMIIGRIEERPFLKEERILYIDTAVTKK
                     GHMKKGYMSLLLKYAEGWCRKRKVPVIELRALKANSPAVSYWKDSGFEEFYIRFRRKV
                     "
     gene            881..1120
                     /locus_tag="PK683_18885"
                     /pseudo
     CDS             881..1120
                     /locus_tag="PK683_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          368 a          234 c          264 g          422 t
ORIGIN      
        1 tcaggagtga aaattataga aatttgtatt cctttcagag aatcattagt tttcagaatt
       61 cagaaagcaa ttatgtctca ttttcgagaa cattttaatt ttcctgggtt ccgccggtca
      121 agaaagtagt ttttccggct ctgtactcga aaactatttc tcctgctttt acatcgcgga
      181 ttttgacctt ccaatttgat cctaaaatca ttcaatgcaa aaaatgttcg cttgctcagg
      241 ggatcttttt ttcgtatata tacagatgaa taagatcaat acagagattc aaatgattca
      301 ggacgggcac agagatcagg caatcatact tctgaatcag tttttcagcc tggtcaattc
      361 tcttcctctg gacgggattt ttgaggtcag accgaatgct gccagaaaaa tgatagatac
      421 atttctcaaa cttcagggga cagataaagt ctgttttgca ggggtatttc aggagagtga
      481 actgctttct atgattatcg ggagaataga agaacgtcca tttttaaagg aagagagaat
      541 actttacatt gatactgctg taacgaaaaa agggcatatg aaaaaaggct atatgtcctt
      601 gcttttgaaa tatgctgagg gctggtgcag aaaaagaaag gttcctgtca ttgaactgcg
      661 cgcactgaaa gccaacagtc ctgcagtctc gtattggaag gattccggat ttgaggagtt
      721 ttatatccgt ttcagaagaa aagtctgagc ttctcaatct gagacaagtt tagttctgtc
      781 tgggggattt ctgttcttgc acttcatttc tttttatata cagtatattc cctgtatgca
      841 acgtatatgc caaggagaaa taaaatgctg agaagtccgg ctgctttttc gtacagggtc
      901 agtctatgct gaaaatgtga cagtccttat ccatggaaag ttcagttcca atcacagcgg
      961 aaccgggaca gacagggttc agacaaatat ctggacagtt atgaacaatg tctgtaatac
     1021 gaattcctgc aagattgtct gccacagtga cggatgttat gcagttgaat attggctttc
     1081 caatttaggc aataccgctt ccggtctggg attttccatg aagttcattc ttgctgaact
     1141 gttcttcttt ctttaatatc tgattgacac ttttttcttt tcatataaaa ccagcctatt
     1201 gtctgcagga catattttat attttgaatg atgtatactc attttcataa ataattgcgc
     1261 cttttccggc tgacagactt gattttac
//
LOCUS       DAOQGJ010001477         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85405_length_1286_cov_3.840780, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001477 DAOQGJ010000000
VERSION     DAOQGJ010001477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85405_length_1286_cov_3.840780"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PK683_18890"
     CDS             <1..520
                     /locus_tag="PK683_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10576.1"
                     /translation="TELEHPELKALNSHNTTSGRVDDNGGYLETTYKYGFPEVTLKTK
                     EYFDAEGKLKGYQEDTGGGYTTPYASLAMPSVIPYATGNYSLDDLNIGLTTAAELTDG
                     ESFVIVLDGVDPNTDNAAILGEGIKNTADTDIEYWGAEAQISTADVWKTGYDSNNEPY
                     YILLSSTGIKKI"
     gene            complement(614..>1286)
                     /locus_tag="PK683_18895"
     CDS             complement(614..>1286)
                     /locus_tag="PK683_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10577.1"
                     /translation="HPSEVMSLIKDTEQTEYFSVGEYWQAASVQDDPMFRRYGGSERE
                     RIVGWGKESGCCVFDMILKRELNTGNPSRFKYGINCSTDREEREMAVTLVDNHDTGAS
                     PFSPANGWGQKVWECPSEFKSRAYAFTLSMPGTPCIYWPDLYDWGLTEIRDLVQARKK
                     AGITAGSAWIDLTGSHTGFAGLVCNAEGKETLALSIHSDYAGPKEGFHLACEKKGEWS
                     VWVKN"
BASE COUNT          367 a          300 c          264 g          355 t
ORIGIN      
        1 cactgaactg gaacacccgg aactgaaagc attgaacagt cataatacaa cttcaggcag
       61 agtggatgac aatgggggtt atctcgaaac aacttacaaa tacggctttc ctgaggttac
      121 attgaaaaca aaggaatact tcgatgctga aggaaaactg aaaggttatc aggaagacac
      181 aggcggaggt tataccactc cttatgcctc acttgcaatg ccctctgtaa ttccttacgc
      241 aacaggaaat tattcactgg atgacctgaa cataggactg acaactgctg ctgaactcac
      301 tgacggagaa tcctttgtga ttgttctgga cggagtggat ccaaatacag acaatgctgc
      361 aattctaggt gaaggaataa agaatactgc agatacagat attgaatact ggggagcaga
      421 agctcagatc agcactgcag atgtatggaa gactggatac gattccaata acgaaccgta
      481 ttatattctg ctcagcagta caggaattaa aaaaatctga atctgtttcg gtgtgaatct
      541 gatacagtca aattcacact gttttatata tctatttact gtatgtaaac agatattttt
      601 atcgtgaaca gtatcagttc ttcacccaca cgctccattc gcctttcttt tcgcaggcca
      661 gatggaatcc ttctttcggt cctgcataat cagagtgaat ggaaagtgca agtgtctcct
      721 tgccttccgc attgcataca agcccggcaa atcctgtatg agatcctgtc agatcaatcc
      781 atgcagatcc tgcagtgatt cctgccttct ttcttgcctg aaccagatca cggatctcag
      841 tcagtcccca gtcatacaga tcgggccaat aaatgcaggg agttccaggc atagagagtg
      901 tgaatgcgta ggcacgggac ttgaattcag aaggacattc ccagactttc tgtccccagc
      961 cgttcgcagg agaaaatgga gaagctccag tatcatgatt gtccaccaag gtgacagcca
     1021 tttccctttc ttctctgtca gtactgcagt tgatcccata tttgaatctg gatgggtttc
     1081 ctgtgttcag ctctcttttt aaaatcatat cgaatacgca gcatccggat tcttttcccc
     1141 atccaacaat tctttctctt tctgatcctc cgtaccgtct gaacatagga tcatcctgca
     1201 ccgaagctgc ctgccagtat tctcccactg aaaaatactc agtctgttca gtatctttta
     1261 taagggacat tacctcactt ggatga
//
LOCUS       DAOQGJ010001478         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85435_length_1286_cov_2.935825, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001478 DAOQGJ010000000
VERSION     DAOQGJ010001478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85435_length_1286_cov_2.935825"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..>1286
                     /locus_tag="PK683_18900"
     CDS             488..>1286
                     /locus_tag="PK683_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH10578.1"
                     /translation="MNANEKKRKIMIVDDEPLNLLVLNETLKDTYDLSLIDGGELAVE
                     EAKKNLPDLILLDVMMPDISGHEVCRQLKADSNTKHIPIIFVSAMSDMKDEAYGFTLG
                     AVDYITKPVRPAIVSARVKTHLSLVRMEELERTQLQIIQSLGYAAEYKDDDTGQHVVR
                     MSHYCRLIAKNAGLSGGRVEEIFRTAPMHDIGKIGIPDGILKKSGKLDENEWKIMKNH
                     PLIGAKIIGDHSEGLLRTARMISLSHHEKWDGLKSRRLVWDAVPLWLP"
BASE COUNT          427 a          240 c          316 g          303 t
ORIGIN      
        1 ctgattatca gtacaattgc cggattctta atcaagctgt gttcgattgg aaatcggaga
       61 attattcagt gttttgaaca ggatcagtga atgattctga aaaagtatgg agctttacaa
      121 gtctttctgg ccgcagaatt ggattgacgg ccggtacatt catatagaaa ttgaaaacga
      181 ttgaagcggc tgcagctgtt ccgtccgcat ctccggaaga gactgctgaa aaatcagttt
      241 taatgacttt atttctaaag aaagaactcc taaacaggga ttcagaatga cagagacatt
      301 ctgaaagacc ggacagaaaa ggcagaatag tctgtctgct cagttaagcc gaaagaagat
      361 aagaaagaac tgtcggcaat ctgagcatct ttataagtga gtcattgaca gaatatagac
      421 aggaaccaga ctgtgaaata atcatagtag gaaatctgtt tcgaaaaacc ggatacggaa
      481 aaactggatg aatgcaaacg agaaaaaaag aaagatcatg attgtcgatg atgaaccttt
      541 gaatcttctg gttctgaatg aaacactgaa agatacttat gatctgagcc tgatagacgg
      601 aggggaattg gccgttgaag aggctaaaaa gaatcttccg gatctgattc ttctggacgt
      661 gatgatgcct gatatcagcg gacatgaggt ctgcagacag ctcaaagcgg attcgaacac
      721 aaaacatatc ccgatcatat ttgtatccgc aatgtcggat atgaaggatg aggcttacgg
      781 cttcactctt ggagcagtgg actatattac aaagccggtc aggcctgcaa ttgtatctgc
      841 aagagtaaag acgcatctct ctctcgtaag gatggaagag ctggaaagaa ctcagcttca
      901 gatcattcag agcctcggtt atgcggcaga atacaaggat gacgacactg gacagcatgt
      961 ggtcagaatg agccactact gcagactgat tgcaaagaat gcaggactga gcgggggtag
     1021 agtcgaggaa attttcagga ctgcaccgat gcacgacatc gggaaaatag gcatcccgga
     1081 cggtatactg aaaaaaagcg gaaaattgga tgaaaatgaa tggaaaataa tgaaaaatca
     1141 tcctttgatc ggagcaaaga taatcggtga tcattcagaa gggcttctca gaacagccag
     1201 aatgatttcc ctctcccacc acgaaaaatg ggacggttta aaatctcgca gactcgtctg
     1261 ggatgcagtc ccactttggc tcccaa
//
LOCUS       DAOQGJ010001479         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85439_length_1286_cov_2.878148, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001479 DAOQGJ010000000
VERSION     DAOQGJ010001479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85439_length_1286_cov_2.878148"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PK683_18905"
     CDS             complement(<1..309)
                     /locus_tag="PK683_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10579.1"
                     /translation="MKLKLIYFLCFSAVFLKLYIFGNLYNTAIHRNGQFFGIFYNTQD
                     NLSYISYIKQAEKGFYMFSDLYTSDENIRGLINPLFLLIGKFCGLFHSDPALTLMFIG
                     V"
     gene            complement(412..1137)
                     /locus_tag="PK683_18910"
     CDS             complement(412..1137)
                     /locus_tag="PK683_18910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH10580.1"
                     /translation="MDLKEEEILKDKIFTHWYYVSKWNALKNFVRISDYRETLDIGAG
                     SGIFTKFLQESSSLKKSVCLDIGYSTDFEETYKGRTIEFRRSIDKSDADLVLMMDVLE
                     HIPDDAEFLKQYVSKIKPGTDILISVPAFQFLFSGHDYFLEHCRRYNSKTLINTVEKS
                     GLTVIRYSYFFGLLFPLIAVIRLFGRLKLKISGRKEEAKSDLKLVPPFLNTALIQIHR
                     LELPFFKWNRIAGLTLFCLARKK"
BASE COUNT          422 a          230 c          243 g          391 t
ORIGIN      
        1 gactccaatg aacattaatg tcaatgccgg atcagaatgg aaaagaccgc agaatttgcc
       61 tatcagaagg aagagcggat tgatgagtcc tcttatgttt tcatctgaag tatataaatc
      121 tgaaaacata taaaaacctt tttccgcctg tttaatatag gagatatatg acagattgtc
      181 ctgagtattg taaaatattc cgaaaaactg tccatttcga tggattgctg tattgtaaag
      241 attcccgaaa atataaagct ttaaaaaaac ggctgaaaaa cagagaaaat agatcaattt
      301 cagtttcata taatttacca agatctacaa aaaaatgata atagctgaat gcaacttaat
      361 tttcagaatc aacggttcag cagtctccac tgaagagttt tataatgaag tttatttttt
      421 tcttgccagg cagaaaaggg ttagacctgc aatcctgttc catttgaaga agggaagttc
      481 cagtctgtgg atttgaatta aggccgtatt taaaaaaggg ggaaccagct ttaaatcaga
      541 tttggcctct tcctttctgc ctgatatttt cagtttcagt ctgccgaaga gacggatgac
      601 tgcaatcaaa gggaaaagaa gtccaaaaaa gtagctgtat ctgattacag taagtccgct
      661 tttttctact gtgtttatca gtgttttgga attgtatctc ctgcagtgtt ccagaaaata
      721 atcatgtccc gaaaaaagaa actgaaatgc aggtaccgaa atcagaatat ctgtgccagg
      781 cttgattttg gatacatact gcttcagaaa ttcagcatcg tccggtatat gttcaagtac
      841 atccatcata agtaccagat cagcgtcact tttatctatg gatcttctga actcgattgt
      901 ccttcctttg tatgtttctt caaagtcagt tgaatatccg atatccaggc agacggattt
      961 tttcaggctg gaggattcct gaagaaattt tgtgaaaatg ccggaacctg caccgatgtc
     1021 cagagtctct ctatagtctg aaattctgac gaaattcttc aaagcattcc attttgatac
     1081 atagtaccaa tgtgtaaata tcttatcctt taaaatctct tcttctttca gatccatagt
     1141 ttttcctcag cagaacctat tcgaagacct gctttcaaaa atgcaagtaa aggttgtcat
     1201 tggttgatat gtttttgcag aagaaaaagc cgaagaagac aaagaaaaag aggaaaaggt
     1261 aactccaact aataaatcgg ccgaag
//
LOCUS       DAOQGJ010001480         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85450_length_1286_cov_2.713241, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001480 DAOQGJ010000000
VERSION     DAOQGJ010001480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85450_length_1286_cov_2.713241"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PK683_18915"
     CDS             complement(<1..393)
                     /locus_tag="PK683_18915"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNH10581.1"
                     /translation="MNHRSDELISEKIRKEGLSETLIADFLSKVDKVRRGETGKVDWS
                     TIGDLDEKTDEISLETLQKNFPSKSENLKKLAVIKLNGGLGTSMGLSKAKSLIPIKNG
                     MSFLDIIAKQIQYFRTKYGIEVPLILMDS"
     gene            complement(390..776)
                     /locus_tag="PK683_18920"
     CDS             complement(390..776)
                     /locus_tag="PK683_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10582.1"
                     /translation="MNRMFLLLLLAVGLNAAPVPYSKIEPVLEETWDRSYPVRYSKII
                     KKDVLGKGIMVLRSKSGGEEYLYSFKVFFPRYSFADSKLVAETEGREIIVRLYHNPAD
                     KENPYRIDLGEFTEKYELGKALRWIK"
     gene            1087..>1286
                     /locus_tag="PK683_18925"
     CDS             1087..>1286
                     /locus_tag="PK683_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNH10583.1"
                     /translation="MFILDDQLSFNIHRVSVILRREMIRCFREFDLNPEQWQVIVSLW
                     EKKKLTQTEIIEITLQDAPSGS"
BASE COUNT          319 a          281 c          259 g          427 t
ORIGIN      
        1 actgtccata agaatcagcg ggacttcaat tccgtacttc gttctgaagt attgaatctg
       61 ttttgctatg atgtccagaa aagacatccc gtttttgatt ggaatcagtg actttgcttt
      121 ggacagtccc attgatgttc caagcccgcc gttcagtttg atgacagcca gttttttcag
      181 attttctgat ttggagggaa agtttttctg taaggtttcc aggctgattt cgtccgtctt
      241 ttcgtccaaa tctccgattg tagaccagtc cacctttccg gtttctcctc ttctgacctt
      301 gtctaccttt gaaagaaaat cggcgatcag tgtctcgctc agtccctcct ttctgatttt
      361 ttctgaaata agttcgtcgg aacgatgatt cattttatcc acctcagtgc tttgccaagt
      421 tcgtattttt ctgtaaactc acccagatca atcctgtaag gattttcttt gtctgcaggg
      481 ttgtgataca gtctgactat aatttctctt ccttcagttt ctgcaacgag tttagaatca
      541 gcgaaactgt acctgggaaa aaaaacttta aaactgtaca gatactcctc tcctcctgac
      601 ttgcttctca gtaccataat tcctttgccg agcacatctt ttttaatgat tttagaataa
      661 cgtaccggat aggatctgtc ccaagtttct tcgagaacag gctctatttt agagtagggg
      721 acaggggcgg cattcagtcc gacagccagt aataaaagga gaaacattct gttcatatac
      781 gtacagattt gcaccttcga tgatctatgc aatcctattt ataactaatc gcccatattg
      841 ttgtcaccaa tgtatttcgt cagctatagt tctgtcatct tgacagcttc cggttggtga
      901 gcagcggctt ccaagtgtgc acggcactgc tgcaggaaca gagtcaacgt tgcacaatgc
      961 tttaagattt ctcgctttgc tgctctactg cgttgcgttc gaaataacag agccagaaga
     1021 cagaaaaaat atttgtctgg acaattattt tattgacatt ttttttcaat tctgtccagt
     1081 gtgtttatgt ttattctgga cgatcagctg agctttaata tccacagggt gtctgtcatt
     1141 cttcgacggg aaatgatcag atgtttcaga gaattcgatc tgaatccgga acagtggcag
     1201 gtcattgtct ctctttggga aaagaaaaag ctgacccaga cggagattat tgaaatcact
     1261 ttgcaggatg ctccttccgg atccaa
//
LOCUS       DAOQGJ010001481         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85510_length_1285_cov_5.082927, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001481 DAOQGJ010000000
VERSION     DAOQGJ010001481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85510_length_1285_cov_5.082927"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PK683_18930"
     CDS             complement(<1..1047)
                     /locus_tag="PK683_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10584.1"
                     /translation="MEKNERPSGPVITMKISERHQKYLTPVEKELYSESELPELFLKL
                     KDRSRTVCEMVWGTKKYDAAYVETLIRKENFKERPFDKWGHPLPKLIAGCWNFFKLND
                     GKPETDFRLVKRLYPNENKDCYSVGFMQSYIMHNEISCDNIYTVDLDWAIQHAHFQFL
                     QKYAANEMKDRESVKKAVGSLEIGWSARFDGKKMLASEKAEINSLCFHTAHESCYRTM
                     ENFQGNFSKLKNFQLSLAELHNMKFSFTPGTVPVIFFSNALEELYTSKSEFALLYDHI
                     QSSMQNGSKAVLIHHAAGRGEFGIYELNKSEKGAEMKTVCRDKFMSSPVGDAPAYEYS
                     THLDRKSTQKTSQNC"
BASE COUNT          331 a          266 c          285 g          403 t
ORIGIN      
        1 acagttttga gaagttttct gtgtgctttt tctgtccaga tgagtggagt attcgtaagc
       61 gggagcgtct cctacagggg aggacatgaa tttgtccctg catacggttt tcatttctgc
      121 acctttttcc gatttgttca gttcatagat tccgaactca ccgcgacctg ctgcatgatg
      181 gatcagtact gcctttgagc cgttctgcat cgaagactgt atgtggtcat ataatagtgc
      241 aaactcggat ttggaagtgt acagttcctc cagagcattg ctgaaaaaga ttacaggcac
      301 ggtaccaggc gtgaaactga atttcatatt atgaagttcc gcaagagaga gctggaaatt
      361 tttcagtttt gaaaagtttc cctgaaagtt ctccattgtt ctgtagcagg attcatgtgc
      421 tgtatggaaa cagagagaat tgatttccgc tttttctgaa gcgagcattt tctttccgtc
      481 gaatcgggca gaccatccga tttcgagact tcctacagct ttctttacgg attccctgtc
      541 cttcatctca tttgcggcat atttctgcag aaactggaaa tgagcatgct gtattgccca
      601 gtccagatcg actgtgtata tattatcgca tgaaatttca ttatgcatta tgtatgactg
      661 catgaagcct acgctgtagc agtccttatt ttcattcgga tagagccttt ttaccagccg
      721 gaaatcagtt tccggttttc cgtcattgag cttgaaaaaa ttccagcatc ctgcaatcag
      781 tttcggaagc ggatgtcccc atttgtcaaa aggtctttcc ttgaaatttt ccttccggat
      841 cagtgtctcc acataggcag catcgtattt ttttgtaccc cagaccattt cacagactgt
      901 tctgctcctg tccttgagct ttaaaaagag ttcagggagc tccgattctg aatacagttc
      961 tttttcaact ggggtaagat atttctgatg acgttcagaa attttcatcg tgatgacagg
     1021 accggaaggt ctctcgtttt tttccatttt actgtgaaaa cattgaatga gaagaagtaa
     1081 gggaagcagt ctcagcataa atctagattt tggacagctg gaatatgttc aagtgattat
     1141 ctgcgggaga ttggaattct gagaaaaccg ggtcagggag atcacggctt tctgctttac
     1201 agaaaaggga tgcaagcaga aaacagaact atgaaatcca gacgctcatt caatacttcg
     1261 ggtccgaaca ttccggaaat gcact
//
LOCUS       DAOQGJ010001482         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85522_length_1285_cov_4.100000, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001482 DAOQGJ010000000
VERSION     DAOQGJ010001482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85522_length_1285_cov_4.100000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1262
                     /locus_tag="PK683_18935"
     CDS             129..1262
                     /locus_tag="PK683_18935"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNH10585.1"
                     /translation="MIIRAEKWVNDGFCLGYAEGRPVFILGAVPGEVAEVELISEKKG
                     AIHVKAVKILITEKERITSDCPVYMKCGGCRFRHISYSEEKKLKTSLLQKEFLFRGIA
                     DPGETLEIEIHSADSDGYRNNVQLKGDEAFFGFHAISSNRVVEIPASGCRLLSDELNQ
                     HLHHIRSGKMDFPESGKLRLNFDGTVAEYFRKNSVFEVSGKTFLCPPDGFFQINRFLT
                     SEWLEEIMQFIPEGTKNIVELFSGCGLISIFAAERCEKLYGYEAHDLSCRFAEQNAGR
                     NRIRNAQFKRKDLYRKIPAREFAESNLWIVNPPRNGLTNDIADILREKLPERIIYSSC
                     NYISLARDLKNLQNHYKIIHLSIFDFFPRTPYFETLAVLEREK"
BASE COUNT          405 a          258 c          286 g          336 t
ORIGIN      
        1 tatgaacctt cttctgcaga gagcactccg tctttcagtt tttccattct ggactacagt
       61 cttttttatg tgcggggaga aagaccgaga gaaacttttt attatttttc cgcaggggtt
      121 tcctggaaat gattatcaga gctgagaaat gggtcaatga cggattctgt ctgggttatg
      181 cggaaggcag acctgtattt atactgggag cagttcccgg ggaagtggca gaagtggaac
      241 tgatttctga aaagaaaggt gcaatacacg taaaggcagt gaagattctc attacagaaa
      301 aggaaagaat tacatccgac tgccctgtct atatgaaatg cggaggatgc agattcagac
      361 atatcagtta cagtgaagaa aagaaactga aaacctcgct gctccagaaa gaatttctat
      421 tccgaggaat tgcagatccg ggagaaactc tggagatcga aattcattct gcagattccg
      481 acggatacag gaacaatgtg cagctgaaag gagatgaagc attctttgga tttcatgcaa
      541 tcagttccaa ccgtgtagtg gaaattcctg cttcaggatg cagacttctt tctgatgagc
      601 tgaatcagca tctgcatcat atccgttcag gaaaaatgga ttttccagaa agcggaaaac
      661 tcagactgaa ctttgacgga acggtcgcgg aatatttcag aaagaattcc gtctttgaag
      721 tatccggaaa aacatttctc tgtcctcctg acgggttctt tcagatcaac agatttctga
      781 cctccgaatg gctggaagaa atcatgcaat ttataccaga aggtaccaaa aatattgtgg
      841 agcttttttc aggatgcgga ctcatttcca tatttgcagc tgaaagatgc gaaaaactct
      901 acgggtatga ggcacatgat ctttcctgca gatttgcaga acagaatgcg ggcagaaaca
      961 ggatcagaaa tgcacagttc aaaagaaagg acctgtacag aaaaatcccc gcaagggaat
     1021 ttgcagaatc gaatctctgg attgtaaatc ctcccagaaa cggtctgaca aacgacattg
     1081 cggacatcct cagagaaaaa cttcccgaaa ggatcatcta ttccagctgc aattacatca
     1141 gtcttgcaag agatctgaaa aatctgcaaa atcattataa aatcattcat ctgtccatat
     1201 ttgatttttt ccccagaacc ccctactttg aaacactggc agttctggag agggaaaaat
     1261 gatttcaact ctcaaatgcc ttgac
//
LOCUS       DAOQGJ010001483         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85652_length_1284_cov_3.800651, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001483 DAOQGJ010000000
VERSION     DAOQGJ010001483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85652_length_1284_cov_3.800651"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..792)
                     /locus_tag="PK683_18940"
     CDS             complement(70..792)
                     /locus_tag="PK683_18940"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNH10586.1"
                     /translation="MPKSDRLDKILANLGFGSRSEVRKLILSGKVKVGSETVKDPSFH
                     AEEGSQIFFEDAPVDRKEFHYFIMNKPSGFITATEDRRERTVMEIFPERIRRMKLFPV
                     GRLDKDTEGLLLFTNDGDLAHRMISPKNMVEKVYYAVIEGSLDTEKIQEFETGLVLEE
                     GFVTMPAKLRIISEKENGAETEVTVFEGKFHQVKRMFEAVGCRVTYLKRIQFGSLVLD
                     ETQLQAGKYRELSLEEESLIRL"
     gene            complement(785..>1284)
                     /locus_tag="PK683_18945"
     CDS             complement(785..>1284)
                     /locus_tag="PK683_18945"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420752.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNH10587.1"
                     /translation="RGSLKGAVLCYVGVHNNVTNSLIEITAALGVHLILVTPVANEKA
                     VIDSVLERAKKKGTLSWETDIAKAVSNADYVYTDTWVDMEFFNDPKFAEEKKKRIDLM
                     LPYQINFELMKKTKAKIMHDMPIHSGYEITREAVKSERSIIFQQAENRLDAQKAVMLR
                     LVGVA"
BASE COUNT          336 a          307 c          227 g          414 t
ORIGIN      
        1 gactggactt tgattctgcg gaaacggcaa tggaaaaaaa agtaaaaact attactgtaa
       61 ttaaattcat cataatctga ttagactctc ctcttccaga ctcagttcac gatattttcc
      121 agcctgaagc tgagtttcat ccagcacaag cgatccaaac tgaattcttt tcagataagt
      181 caccctgcac cccactgctt caaacattct ttttacctgg tggaacttcc cttcgaatac
      241 agtcacttcc gtttcagcac cgttttcctt ttccgaaata attctgagtt ttgcaggcat
      301 tgtgacaaaa ccctcttcaa gaacgagtcc tgtttcaaat tcctgaattt tttcggtatc
      361 gagagatcct tctatcactg cataataaac tttttccacc atatttttag gagagatcat
      421 cctgtgtgca agatcaccgt cattagtgaa gagaagcagg ccttccgtat ccttgtcgag
      481 tctccccaca ggaaaaagtt tcatccttct gattctttca ggaaaaatct ccatcactgt
      541 cctttctctt ctgtcttctg tagccgtaat gaaaccggaa ggtttattca ttataaaata
      601 atgaaactcc tttctgtcaa caggagcatc ttcaaagaaa atctgagatc cttcctctgc
      661 gtgaaaagaa ggatctttta cagtttccga acctactttg acttttccgg aaagaatgag
      721 ttttcttact tcactcctgg aaccgaatcc aagattggct aggatcttgt cgagacgatc
      781 cgacttaggc aacgccgaca agcctgagca tcactgcctt ctgtgcatcc agccggtttt
      841 ctgcctgctg gaaaatgatg gatctttcgc ttttcacagc ttctcttgtg atttcatatc
      901 ctgaatgaat cggcatatcg tgcatgatct ttgcctttgt ttttttcatc agctcaaaat
      961 tgatctggta aggaagcatc aggtcgattc tttttttctt ttcttctgca aacttagggt
     1021 cattgaaaaa ctccatatcc acccaagtat cagtgtacac ataatctgca ttgcttactg
     1081 cttttgcaat gtcagtctcc cagctgagag ttcctttttt ctttgctctt tccaggacgg
     1141 aatctatcac tgctttctca tttgcaacag gggtcacaag gataagatga actccgagag
     1201 ctgcagtgat ttcgatgagg gaatttgtga cattgttgtg caccccgaca taacataaaa
     1261 ccgcaccttt cagtgaacct ctgt
//
LOCUS       DAOQGJ010001484         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85693_length_1284_cov_2.512612, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001484 DAOQGJ010000000
VERSION     DAOQGJ010001484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85693_length_1284_cov_2.512612"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(805..>1284)
                     /locus_tag="PK683_18950"
     CDS             complement(805..>1284)
                     /locus_tag="PK683_18950"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNH10588.1"
                     /translation="SLLLILSCTWILGGIAFLVFESYFRKQPEAETKEISISQAVYIG
                     LFQCIALIPGVSRSAMTILSGRYFGLSKEKATEYSFFLALPVLIIASGYKLMKHRHAL
                     NSENIPVLGLGFFLTFVFSIVIIRAFLEYVRKYDFKPFGWYRIVLGTAAAAYFFMKN"
BASE COUNT          386 a          271 c          287 g          340 t
ORIGIN      
        1 atgtttaccg ccgtataaat caactctttt aatttcatcg aaacccaaag gatttaattt
       61 atttatcata taagttgcat atttcaagat tcttccgata aaatcttcca caaaagttgt
      121 atgatcacct tcggcttcag gattcggttt tattttatta acttttgcca tatctgaacc
      181 atagcaaaaa gcactaatat tatgaatagt aaaattcaat tattaaagca caagcagacc
      241 tgtcaatagg gaaagggtga gagagttttt cagacggaag aaaatcgtag cagaattgat
      301 tataatgatg ggtcgggaaa gggaaagttt tttttgaacc ggcaaccttt tgcagagctt
      361 acagcgggca aaaggttggc ctgctgggtt gcgagcttcg agagcaaggg gggaaacctt
      421 gtggaagccg taggcgggca gaaaattggc ctgtgacccc ccattcacct agcggtgaat
      481 ccaagcaggc ctcccttcgg tcgccgggga atccgaggta aagctttctg ggttcacagc
      541 aaagcatcct tatccgcccc tctccataac gaaatatatt aacgttatgg ccgccctttg
      601 cgctgatgcg caaacccagc aggcctccct tcggtcgccg gggaaagcgg agctggggtg
      661 aggtaagcat gttgtaccct ctgcagaaaa cattctcttc aaaaactgtg tctttttctc
      721 aggatacttt aagattcctc actgcgttcg gaatgacaaa ggggatttct ttcgaaactg
      781 cggatcagac tgcactataa aaaattaatt tttcatgaaa aaatatgccg cagcggcagt
      841 gcctagaaca atcctgtacc agccgaacgg cttgaaatcg tattttctca catactcaag
      901 aaatgctctg atgatcacga ttgaaaaaac gaatgtcaga aaaaaaccga gaccgagtac
      961 cggtatgttt tctgaattca gtgcatgtct gtgtttcatc agtttgtatc cgcttgcaat
     1021 aatgagcaca ggaagtgcca ggaaaaaaga atactctgtc gccttttcct tggaaagacc
     1081 gaaataacgt cctgaaagaa tcgtcatcgc agaacgggat actcccggaa tcagagcaat
     1141 gcactggaag agaccgatat acactgcctg agaaatgctg atctctttgg tttctgcttc
     1201 tggctgtttt ctgaaatagg attcaaatac aagaaatgca attcctccga gtatccatgt
     1261 gcaggaaagg atcagaagaa gaga
//
LOCUS       DAOQGJ010001485         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85713_length_1284_cov_2.263629, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001485 DAOQGJ010000000
VERSION     DAOQGJ010001485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85713_length_1284_cov_2.263629"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK683_18955"
     CDS             <1..258
                     /locus_tag="PK683_18955"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNH10589.1"
                     /translation="LLKGPVTVFCSAERELYYFSAPNPRLAVMGTGDIFSGLLAFFLS
                     KTEDMDYSVQASLSVFRKTEKIGKKSSDASDILNFIEENFS"
     gene            255..866
                     /locus_tag="PK683_18960"
     CDS             255..866
                     /locus_tag="PK683_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10590.1"
                     /translation="MSKGYHSRSPEELRAYLDKWKNKDRKKNWRNILIFLDLLLLMFV
                     FYLVSKKLGSGTEYRKSSEKKIFESTEWQLTRSNTEISEQLGYFLFAKNLSSTPIVPD
                     RLKAVLTLKSETGLICKTETFVLPKQKILPNERAYYSLEFHAGKHSEYPAECSGLFRH
                     PPFPKTVYNAFDFSKKYRVDMILQLKTGEAELKFVIEDERLRF"
     gene            complement(855..>1284)
                     /locus_tag="PK683_18965"
     CDS             complement(855..>1284)
                     /locus_tag="PK683_18965"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH10591.1"
                     /translation="FEKISIFSGASEDFIEDIVMVLKPAVFTPGDYIFKKGEVGSSMY
                     FIAQGRVEVLNDFSKEVIAVISEGGFFGEFALLESRPRSATVRAVDFCDLYSLDRDSF
                     YRVLQNYPDFHEKIKKIYKDRDGKMKKFGNVKGSRPKSKS"
BASE COUNT          376 a          277 c          265 g          366 t
ORIGIN      
        1 ctcctgaaag gaccggtcac tgtcttctgt tctgcagaaa gagagcttta ttatttcagt
       61 gcaccgaacc cccgtctggc cgtcatggga acgggagata ttttttccgg tctgcttgcc
      121 ttctttcttt caaagactga ggatatggat tattcagttc aggcatcgct ctctgtattc
      181 agaaagacgg aaaaaatagg gaaaaaatct tcggatgcgt ccgacatact gaattttatt
      241 gaggagaatt tttcatgagc aagggatacc attcaaggtc gccggaagaa ctgagagcat
      301 atcttgacaa atggaaaaac aaggacagaa aaaaaaactg gagaaacatc ctgatttttc
      361 tcgaccttct tcttctgatg tttgttttct atcttgtatc caaaaaactc gggtcaggca
      421 ctgaatacag gaaatcctca gagaaaaaaa tattcgaatc cactgaatgg cagctgacca
      481 gatcaaatac ggaaatctcg gaacagctcg gttattttct gttcgcaaag aatctgagca
      541 gtacacccat tgtacccgac aggctcaagg ctgtgctcac tctgaaatct gaaacgggac
      601 tcatatgcaa aacggaaact tttgtgctcc ccaagcagaa aattctgccg aatgaaagag
      661 catattacag tctggaattt catgcaggga aacattctga atatccggcg gaatgctcag
      721 ggctttttcg gcatcctcct tttcctaaaa cagtttataa tgcttttgac ttttcaaaaa
      781 agtacagagt ggatatgatt cttcagctga agacaggaga ggctgaactg aagtttgtga
      841 ttgaggatga aaggttaaga ttttgatttt ggcctgctgc cttttacatt tccgaacttt
      901 ttcatttttc cgtctctgtc cttatatatt ttcttgatct tttcatgaaa atcagggtaa
      961 ttctgaagga ctctgtaaaa actgtccctg tccagactgt aaagatcgca gaaatccact
     1021 gcacgcacgg tggctgatct gggtctgctt tccaaaagag caaactctcc gaaaaaccct
     1081 ccttcagaaa tgactgctat gacttctttg gaaaaatcgt tcaggacttc cactctgccc
     1141 tgagcgatga aatacattga acttcccacc tcgcctttct tgaagatata gtcgccaggc
     1201 gtgaaaactg caggcttgag caccattacg atgtcttcaa taaagtcttc agacgcgccg
     1261 gaaaatattg aaatcttctc aaat
//
LOCUS       DAOQGJ010001486         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85784_length_1283_cov_3.385179, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001486 DAOQGJ010000000
VERSION     DAOQGJ010001486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85784_length_1283_cov_3.385179"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1283)
                     /locus_tag="PK683_18970"
     CDS             complement(<1..>1283)
                     /locus_tag="PK683_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNH10592.1"
                     /translation="YIEDRAEQKQLIEYNILAAKKAKASTAYKPALYYIMKAAEIADH
                     SKMNDWKDNYSIIFEIYKDKAELEYLNGNFDQSEKDINIILENSDSAVDKAAVYKILI
                     MQYTLRAKYKEAMVILRKALSLLGVSLPEEDLQQSFLSEVEKTKMLLGTRQVSSLINE
                     KENKDREKVLAVQLLTTSTSASYLSSPDLWCVIVMKTVNLFLEYGNRPESYGYSCFGI
                     LQGVAFGNFKLGYEYSNLAYKISEKFNNASERCKAANVLANLTMPWVKPLKECEIVNK
                     EGLQDSLNSGEYIHGGYITMNAPVNFYFQGKRMDELTTEITKLHQLSRKMKSNVAVDN
                     TLGILQIIGNLTGETSDETDFDTEELKESEFLENCGSRESVFAVCIYKIMKAQLLVVY
                     GKYEEALAIFEETKSMLAFVTGIVVVAEHNFYYTL"
BASE COUNT          323 a          294 c          258 g          408 t
ORIGIN      
        1 caagagtata ataaaaattg tgctctgcca caacaacaat ccctgtgaca aatgcaagca
       61 ttgactttgt ttcttcaaaa atggctagtg cttcctcata ttttccgtag accaccagaa
      121 gctgtgcctt catgatttta tatatgcata ctgcaaatac actttctctt gatccgcagt
      181 tttcaagaaa ctccgactct ttcagttctt ccgtatcgaa atcagtttca tcggatgtct
      241 caccggtcag atttcctatg atctgaagaa tgcccaaagt gttgtcgaca gcaacattac
      301 ttttcatttt ccggctgagc tgatggagtt tcgtaatttc agtcgtaagc tcgtccattc
      361 tcttgccctg aaaataaaaa ttcacagggg cgttcattgt aatgtatccg ccgtgaatgt
      421 attctcctga attgaggctg tcctgaagac cttccttgtt gactatttcg cattctttca
      481 gcggttttac ccagggcata gtcaggttcg ccagaacgtt tgcagccttg catctttcac
      541 tggcattgtt gaatttttca ctgatcttgt aagccagatt agaatactca tagccgagct
      601 tgaaatttcc gaaagcaaca ccctgcagaa taccaaagca ggaatatccg taagattcag
      661 gtctgtttcc gtattcaaga aacagattga ctgtcttcat cacaatcacg caccagaggt
      721 cagggctcga aaggtaggat gccgaagtgg atgttgtcag aagctgcact gcaagaacct
      781 tttctctgtc cttgttttcc ttctcattga tcagagagga tacctgtctg gttccaagga
      841 gcattttggt cttttccacc tcagagagga aactctgctg cagatcttct tccggcagag
      901 agactccgag aagagagagt gccttcctga gaatcaccat cgcttccttg tatttggcgc
      961 gaagggtata ctgcataatt agaatcttgt aaactgcggc cttgtccact gccgaatctg
     1021 agttttccag aattatgtta atgtcttttt ccgactgatc aaaattgccg ttcaaatact
     1081 ccagttcagc cttgtccttg taaatttcaa atatgatgct gtaattgtcc ttccagtcgt
     1141 tcattttgga atgatcagca atttcagcag ccttcattat ataatagagc gcaggtttgt
     1201 aagcagtgga ggccttcgct tttttggcag cgagtatgtt gtattctatc agctgcttct
     1261 gctcagccct gtcttcaatg taa
//
LOCUS       DAOQGJ010001487         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85892_length_1282_cov_3.190709, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001487 DAOQGJ010000000
VERSION     DAOQGJ010001487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85892_length_1282_cov_3.190709"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1282
                     /locus_tag="PK683_18975"
     CDS             <1..>1282
                     /locus_tag="PK683_18975"
                     /inference="COORDINATES: protein motif:HMM:NF014489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10593.1"
                     /translation="NLLFSGQAIKNAGLHHQVVVTLMELPPGASGAHDPEFYMKNIRD
                     ILDAGVPFDSICFKDSSGVANPSKVYETIRQARKLLGEKVVLWMHTHDTASIGISQYK
                     AGIEAGLDGICLARSPISGGTSQPDLLAMWHTLKGTNFTLDIDVNKILEANSILKDCL
                     KDYFFPPEAMGISPEVLFSPMPGGALTANTLMMRDTGTLHLYPKVIESMAECIAKGGY
                     GTSVTPVSQFYFQQAYANVTQGNWKKITDGYGKMILGYFGRTPVKPDEAVVKIASEQL
                     KLPVFSGDPLDRLEPGIPKARKILEDNQLPVNDENIFIIGCFATPGGNKGLDFLKGKV
                     PVNVKKNTPESSEKKTDTKEAVTVSKPSTVRSSGNYKITVNGKVYDVSVEETTGQVHS
                     IQEIGAPHSVAPVKSVSESVIEVKTNLPGNIYQIA"
BASE COUNT          388 a          276 c          292 g          326 t
ORIGIN      
        1 ggaatctgct cttttcggga caggccatca aaaatgcagg actccatcat caggttgttg
       61 taactctgat ggagctccca ccgggtgcat ctggggctca tgatccggaa ttttatatga
      121 agaacattcg ggatattctg gatgcaggag ttccttttga ttccatctgc ttcaaggatt
      181 catccggtgt agcaaatcct tccaaagttt atgagacgat cagacaggcg agaaaactcc
      241 tgggtgagaa ggttgttctc tggatgcata cacatgacac agccagcata ggcatcagtc
      301 agtacaaagc cgggatagaa gccggactgg acggaatctg cctcgcacgc tctccgattt
      361 ccggaggaac ttcgcagccc gatcttctgg caatgtggca tacactcaaa ggtaccaatt
      421 tcactttaga catcgatgta aataaaattc tggaagcaaa ctccattctc aaggattgtc
      481 tgaaagacta ttttttccct ccggaagcaa tgggaatctc tcctgaagtt cttttttctc
      541 cgatgcctgg aggtgctctt acggcaaata cacttatgat gcgggatacc ggcactcttc
      601 acctgtatcc gaaagtcatt gagtcaatgg ctgaatgtat tgccaaagga gggtatggaa
      661 cttccgtaac tccagtttca cagttttact tccagcaggc ttacgcaaat gtaactcagg
      721 ggaactggaa aaaaattaca gacggttatg gaaaaatgat tctcggttac tttggaagaa
      781 ctccagtaaa accggacgaa gcagtggtaa agattgcttc cgaacagctg aaacttcctg
      841 tattttcggg tgatcctctc gacagactgg aaccgggaat tcccaaagca aggaaaatat
      901 tagaagacaa tcaactgccg gtaaacgatg aaaatatctt tatcattgga tgctttgcaa
      961 ctcccggcgg aaataaggga ctggattttc tgaaaggcaa agtcccagta aatgtaaaga
     1021 aaaacacccc tgaaagttcg gaaaaaaaga cagatacaaa agaagcagtc actgtttcaa
     1081 aaccctcaac ggtcagatcc tcagggaatt ataaaattac tgtcaatggg aaagtttatg
     1141 atgtttccgt ggaagagaca acaggacagg tacattctat tcaggaaatt ggagctcctc
     1201 attcagtggc accagtcaag tctgtttcag aaagcgtcat tgaggtaaaa accaatcttc
     1261 ccgggaatat ttatcagatt gc
//
LOCUS       DAOQGJ010001488         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_85938_length_1282_cov_2.290954, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001488 DAOQGJ010000000
VERSION     DAOQGJ010001488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85938_length_1282_cov_2.290954"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PK683_18980"
     CDS             complement(<1..712)
                     /locus_tag="PK683_18980"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNH10594.1"
                     /translation="MHSTGSLEKLWHLLDREQIINKSNLEKMLAHHLEYTVGKHRNNT
                     TVSDIYKALAYSVRDILIDRSNETQAAYRKNNPKRVYYLSLEFLMGRTLMNAIMNLGI
                     YNIVQEVISDFGLDLHEIEECEHDAGLGNGGLGRLAACFMDSLATLNLPGYGYGLRYE
                     YGIFNQLIEDGSQIERPDQWLSNGNPWEFPRPEASYPVNFYGHVERTVSPAGRVVWNW
                     KPSEVILAGGAATHGLVNA"
     gene            complement(1004..1222)
                     /locus_tag="PK683_18985"
     CDS             complement(1004..1222)
                     /locus_tag="PK683_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10595.1"
                     /translation="MEYFHAVKSALVYPGKESDVIGMFYGTGLGSEEKECSVLQFSVP
                     EKSDSMSLIRTWQKQICRKVSEWAVGKP"
BASE COUNT          334 a          324 c          258 g          366 t
ORIGIN      
        1 ccgcattcac caagccatgc gtggcggctc cccctgccag aatgacttct gaaggcttcc
       61 agttccatac aacccttcct gcaggactga ccgttctttc cacatgaccg taaaaattga
      121 caggataaga cgcttccggt cttggaaatt cccaaggatt tccgttggaa agccactggt
      181 caggtctttc aatctgggaa ccgtcttcaa tcagctgatt gaaaatccca tactcgtatc
      241 tcagaccgta cccgtatcct ggaagattca gagtggccaa tgaatccata aaacaggctg
      301 ccagtcttcc cagacccccg tttcccagac ctgcatcatg ctcgcattct tcaatttcat
      361 gcagatccag accgaaatcg ctgatgactt cctgaacgat attgtaaatc cccagattca
      421 taatcgcatt catcagtgtt cttcccatca gaaactcaag agagagatag tatactcgtt
      481 taggattgtt ttttctgtaa gcggcctgag tctcattgga tctgtcgatc agtatatccc
      541 tgactgaata ggcaagagcc ttgtaaatat cggaaactgt cgtattgttt ctgtgttttc
      601 cgacagtgta ctccagatga tgcgcaagca ttttttccag attgcttttg tttataatct
      661 gttccctgtc cagtaagtgc caaagttttt ccaatgatcc tgttgaatgc atgatgtctc
      721 ccaatgctga tataaaattc ttgaaatgta agtcagtcgc ctgatgaaag acgatgccga
      781 gccccgaacc tgcgcctgaa tctcaaaaaa cccgaagtcc gggctgtcag agacacagaa
      841 attgctgggg catcaaggca ttcttctgca cagtacctgc atattttttc aaaaactctt
      901 tgaactcgga atattttaat ttgctgtccc tgaatacaat tctttttttt cttttccggt
      961 ctgtaaaatt aaaagaccag cgacagggcc tgacccctgc ggattatggt tttcccactg
     1021 cccactcgga aacctttctg cagatttgtt tctgccaggt tcggatgagg ctcattgaat
     1081 ctgatttctc cgggacggaa aactgcagaa cagaacattc tttctcttca gagccgagcc
     1141 ctgtcccgta aaacataccg attacatcag attcttttcc aggatacaca agtgcagact
     1201 tcactgcatg gaaatattcc atatatacag acatctgacg gagatcctca ggggacggat
     1261 tggaggcgtt cagaatccgc ta
//
LOCUS       DAOQGJ010001489         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86121_length_1280_cov_3.733061, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001489 DAOQGJ010000000
VERSION     DAOQGJ010001489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86121_length_1280_cov_3.733061"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1280)
                     /locus_tag="PK683_18990"
     CDS             complement(<1..>1280)
                     /locus_tag="PK683_18990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNH10596.1"
                     /translation="TPESFRKDAYDYFALLFEKGEISFETNLLTKDQKEISVTLDAVK
                     LSDSEYMAYCRDVTELKKMEKENQNIQQKLEAFFSQSLDGFFFMMLDEPVVWNSETDR
                     EKLLEYAFSHQKITRVNQAMLDQYRASADQFIGLTPEDFFEHSIEEGKKIWRELFDRG
                     KLSVETKERKFDGTEMIIEGYYICLYDSDGKIAGHFGIQRDVTEKKNAEKELISAKEK
                     AEESERLKTAFLANMSHEIRTPLSAIIGFSGLLAESDDLNAEEKKEFADIIKDSAAGL
                     LTLINDILDISRLDTGLLELRSEKFALNSLLKEIRTVYRLQKDKSDKKDIEIIMECGL
                     QDEKSTVICDRVRLHQIISNLMSNAMKFTEIGYIKFGYSLKNGFLEFFVKDTGSGIPA
                     EKQSIIFERFRQADEIHDRKYGGAGLGLSIVKGLV"
BASE COUNT          312 a          312 c          236 g          420 t
ORIGIN      
        1 accagtcctt ttacgatgga aagtccaagc cctgccccgc cgtattttct gtcatgtatt
       61 tcatcagcct gtctgaatct ttcaaagatg atactctgct tttctgcagg aattcctgag
      121 cctgtatcct tcacaaagaa ttccagaaat ccgttcttca gtgaatagcc gaatttgatg
      181 tagcctatct ctgtaaattt catcgcgttg gacatcagat tggatatgat ctgatgaaga
      241 cgaaccctgt cgcagatcac agtgcttttt tcatcctgaa gcccgcactc cataatgatt
      301 tctatgtcct ttttatctga tttatccttc tgcagacggt atactgtcct gatctccttc
      361 agaagagaat tcagtgcgaa cttttcagac ctgagctcca gaagtcccgt atcgagtctg
      421 gatatgtcca gaatgtcatt gatcagagtg agaagtcctg cagcactgtc cttgataatg
      481 tctgcaaact cttttttttc ctctgcattc agatcatcac tttcggcaag caggcctgaa
      541 aaaccgatga tcgcactcaa cggagtcctg atttcgtgag acatatttgc cagaaaggca
      601 gtcttcagcc gttcgctctc ttccgctttt tcctttgcag aaatcagctc cttttctgca
      661 ttcttctttt cagtcacatc tctctgaatc ccgaaatgtc cggcaatctt gccgtccgaa
      721 tcataaaggc atatataata tccttcaatg atcatttctg ttccgtcaaa tttcctttct
      781 tttgtttcta cagaaagttt tccacggtcg aacagttccc tccagatttt ttttccttct
      841 tcaatgctgt gctcaaaaaa atcttcagga gtcaggccga tgaactgatc tgcggaagct
      901 ctgtactgat cgagcattgc ctgattcact cgggtgattt tctgatgaga aaatgcatat
      961 tccagaagtt tttccctgtc tgtctctgaa ttccagacaa ccggttcatc gagcatcatg
     1021 aagaagaatc cgtccagaga ctgactgaag aatgcctcca gcttctgctg tatattctga
     1081 ttttccttct ccattttttt cagctcagta acgtccctgc agtatgccat atactctgaa
     1141 tcggaaagct tcactgcatc cagagtgacg gatatttctt tctgatcctt tgtcagaaga
     1201 ttcgtttcaa agctgatttc acctttttcg aagagaagag caaaataatc ataagcatct
     1261 tttctgaatg attcgggggt
//
LOCUS       DAOQGJ010001490         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86123_length_1280_cov_3.506122, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001490 DAOQGJ010000000
VERSION     DAOQGJ010001490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86123_length_1280_cov_3.506122"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PK683_18995"
     CDS             complement(<1..403)
                     /locus_tag="PK683_18995"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HNH10597.1"
                     /translation="MQSTIFDEYNSLKKNFIKAMFKGPVSQEVLAELGGMIRSSLSSE
                     NSVRNIFAVFIEMAQNILHYSEEKDENGSGIGIVKVHDAEGIVVVEAGNQILSSKAEG
                     IKNKIDHLNTLDKEGLRKFYQQKLREPRPDGS"
     gene            complement(1040..>1280)
                     /locus_tag="PK683_19000"
     CDS             complement(1040..>1280)
                     /locus_tag="PK683_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10598.1"
                     /translation="VSYMKLDLELFGFFQPGQGQNNTVVFQCYTSDGLKCTTTTSSAQ
                     CYTYDSVNCITTNAGKNITVKATVNCSAKNIVPSS"
BASE COUNT          365 a          283 c          226 g          406 t
ORIGIN      
        1 tgcttccatc aggtctaggt tctctaagtt tctgctgata aaacttgcgc aagccttcct
       61 tgtccaaagt attcaaatga tctattttat tttttattcc ctcagctttc gaactcagga
      121 tttggttgcc tgcctcgaca accacaatgc cttcagcatc atgcaccttg acaattccta
      181 taccgctacc attttcatcc ttttcttctg aataatggag gatattttgt gccatttcta
      241 taaaaacggc aaatatgttt ctgacagaat tctcactgct taaactggaa cgtatcattc
      301 cgcccaattc tgccaaaacc tcctgtgaaa caggaccttt gaacatagcc ttaataaaat
      361 tcttcttcag agaattgtat tcatcaaata tcgtgctttg catgtgaacc catactacaa
      421 atcatatctt ttaatgatct gtcaacaaaa aaaagctttt ttgtttctaa tcattgattt
      481 tctgaaataa acgagaaaat ccaataaata atcaaaccgg aataccattt tatggagaca
      541 ggacagctga tgcccgagga tttttctttc catatttcgg atcagaatga acaaagctta
      601 aaactgttct gcattgaccg attcgtttta gaggaattgg atgaatcaat tcagaaagca
      661 agctctgctt tgcagtaact gtttgattca acttacgcct tcggctgcct tcagctgcct
      721 cagactgcat atctgtcggc caatacttta caattaatga taatttgtaa tgctttcagc
      781 tgcctgccct gagcaagttc aaataggtcg aaggagcact gttacgaggg ttatagttcg
      841 atatagccca ctaatgaccc cggcctttca tcggcttgcc gacggcttga ggactcccaa
      901 atcaggaaac agctgttttg ctgattcatc tcaaactttt aaactgccgg attcctctaa
      961 tacttcgaaa aaattacagg tcccataaat ttactctgtg actgctttta cacagtcact
     1021 tcactgagaa aaatccagtc tagctggatg gaacaatatt tttagcagag cagttgactg
     1081 tcgctttgac ggtaatgttt ttacctgcat ttgtggtaat acagttcaca ctgtcataag
     1141 tatagcattg ggcagaggat gtggttgtag tacattttaa cccgtccgat gtgtaacact
     1201 gaaagactac tgtgttgttt tggccctgtc ccggctggaa aaatccgaaa agctccaaat
     1261 ccagtttcat ataggaaacc
//
LOCUS       DAOQGJ010001491         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86231_length_1279_cov_3.805556, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001491 DAOQGJ010000000
VERSION     DAOQGJ010001491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86231_length_1279_cov_3.805556"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..426)
                     /locus_tag="PK683_19005"
     CDS             complement(82..426)
                     /locus_tag="PK683_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10599.1"
                     /translation="MWRITKFMKFLIIGALAIGVFSAGAMVLWNALIPELFHGPVLTY
                     WQTIGLLVLSHIFFRGGHFGGGHRWRHEAWKRKMQSRMEGMNPEQKEKFLSELGGHGC
                     CHGHRHREKETV"
     gene            complement(432..935)
                     /locus_tag="PK683_19010"
     CDS             complement(432..935)
                     /locus_tag="PK683_19010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH10600.1"
                     /translation="MAIRQDGKMDSLIQRLINFVKKRVSSLEDAEDIVQDVFSVFLEG
                     NLAETVEDLSAWLFRAARNRIIDLYRKRSSSADTGQLSGTEEFETEQEYSTRFWKELE
                     TALSELPEEQRAVFEQNELEGKSFREISEETGENINTLLSRKRYAVLYLRERLRELYD
                     EMTEETR"
BASE COUNT          337 a          320 c          229 g          393 t
ORIGIN      
        1 acatcgctcg gtgaccggcg gtgaaaattg tatcgaggta tgcttttaca caaccccgat
       61 cctgcagccg cttcactcca ttcagactgt ttctttttct ctatgtctgt gaccgtgaca
      121 gcaaccgtgt ccgcccagtt cagaaagaaa tttctccttt tgttcaggat tcatcccttc
      181 cattctgctc tgcatctttc tcttccaggc ctcatgtctc catctgtgac ctcctccaaa
      241 atgccctcct ctgaaaaaga tatgactcag cacaagaagc cctattgtct gccagtaagt
      301 caggacaggt ccgtgaaaca gctcaggaat gagagcattc cagagaacca tcgcacccgc
      361 gctgaaaaca cctattgcaa gtgcacctat aattaagaat ttcataaatt ttgtaattct
      421 ccacatactt attacctcgt ctcttcagtc atttcgtcgt acagttccct aagtctttcc
      481 cgcagataaa ggacagcgta tcttttccgg gacagaagag tattgatatt ctctcctgtc
      541 tcttcagaga tttcgcggaa actttttcct tccagctcat tctgttcaaa tacagctctc
      601 tgttcttccg gcagttcaga aagggctgtc tccagttcct tccagaagcg tgtgctgtat
      661 tcctgttcag tttcaaattc ttcagtaccg gataactgtc cagtatctgc agaactgctc
      721 cgcttacggt aaagatctat gatcctgttt ctggcagcgc gaaaaagcca ggcagacaga
      781 tcctccacag tctccgccag atttccttct aaaaagacag agaatacatc ctgtacaata
      841 tcctcagcat cttcgagaga gctgactctc ttctttacaa aattgatcag tctttgaatc
      901 agtgaatcca tcttgccgtc ttgtcttatt gccaaaattg cctgttccga cattctgtaa
      961 aggatgacgc aaactgaaga tttttattgt atgatttctg attttttttc cggataccgc
     1021 tgcctgactg aaaaattttt tctgtcaaaa aaggagtcct ccgttttctg caatatccca
     1081 tttgcaagag ccgaattgtc agttgacata atttgagtaa aactgagatt agaagaaatt
     1141 ctatgcagtc ttatacttta aagcggcttc ctctctcaca gaatatgata ataaaagata
     1201 taatcccctg atatgcagac aaatcctgat cctgaaattc tgatccagct ggtgaatatg
     1261 gaaatgcctt tcggaaaat
//
LOCUS       DAOQGJ010001492         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86362_length_1278_cov_3.580540, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001492 DAOQGJ010000000
VERSION     DAOQGJ010001492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86362_length_1278_cov_3.580540"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PK683_19015"
     CDS             complement(<1..751)
                     /locus_tag="PK683_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10601.1"
                     /translation="MRNRNFILNILFFVILISVILYRAVDRIWITEDAFISFKHIDNY
                     FAGNGLSFNKWNRLECFTHPLWVFLILFVKFFTSLAHFQIAVVLGILFSVLSQAVLFY
                     YFMKKYTMQKLHLFLLPSVYAMHLGFIDFTTSGLENSLTYFLLVLFYIQVLDRKKFFS
                     PGISAVLGLLYLVRPEFILFAVYYSFIYVLSALKNRLGIRAFILFFIPLAFFVGGYHI
                     FRYFYYNDIFPMSYYAKAGGEVNISDGIFYLQ"
     gene            complement(751..>1278)
                     /locus_tag="PK683_19020"
     CDS             complement(751..>1278)
                     /locus_tag="PK683_19020"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNH10602.1"
                     /translation="KSKVSLRILADLLIALADIRMGGILSPVFIKFCITGFAGVLVNL
                     FGQWAGMEIIEKYFLHTIHDNRYTVSSFSVLFGFVMSVLFNFVLNNSWTFREKRLNTK
                     SAILVSFPRFAAVTVLGFLVQISVWRSFAGICSENQIFGFYGCSFSGNLAGILSAAFG
                     NYYLSDRFAWSKRSS"
BASE COUNT          462 a          274 c          245 g          297 t
ORIGIN      
        1 gctgcaggta gaagatcccg tcggaaatat tgacctcccc tcctgccttt gcataataac
       61 tcataggaaa aatatcatta taataaaagt aacggaatat atgatatccc ccgacaaaaa
      121 aagccagcgg aataaagaac aggatgaatg cacggattcc aagtctgttt ttcaatgcac
      181 ttaagacata aataaatgaa taatagactg cgaaaagaat aaactcaggt cttacaagat
      241 acaggagtcc gagcacggca gaaatcccag gggaaaagaa ttttttcctg tccagtacct
      301 gaatgtaaaa cagcacaagc aggaaatatg taagtgaatt ttcaagacct gaggtcgtaa
      361 aatcaatgaa acccagatgc atagcatata ctgagggaag cagaaaaaga tgaagcttct
      421 gcattgtata ttttttcatg aaataataaa acagaactgc ctgagacaga acgctgaaga
      481 gaatgcccag gacaaccgcg atctgaaaat gagccagact agtaaaaaac tttacgaaaa
      541 gaatgagaaa tacccaaaga gggtgagtga agcattccag cctgttccat ttgttgaagc
      601 tgagaccgtt tcctgcaaag taattgtcta tatgcttgaa actgatgaat gcatcttccg
      661 taatccaaat tctgtccacg gccctgtaaa gaatcactga aatcagaatc acaaaaaaaa
      721 gaatattcag aataaaattt ctgttcctca ttaactgctc cttttcgacc aggcaaacct
      781 gtcgctcaga taataattgc cgaatgccgc agagagtatc cctgcaagat tcccggaaaa
      841 actgcatccg taaaaaccga agatctgatt ttcactgcag atgcctgcaa aacttctcca
      901 tacagagatc tgcacaagaa accctagaac agtgacagcg gcaaaccggg gaaaactgac
      961 taagatggca ctctttgtat tcagacgttt ttcacggaac gtccaggaat tgttcaggac
     1021 aaaattgaaa agcacgctca ttacaaatcc aaaaagcaca ctgaaggatg atactgtata
     1081 tctgttatca tgtattgtat gcaaaaaata tttctcaata atttccatcc ctgcccattg
     1141 cccgaaaaga tttaccagca ctcccgcaaa tccggtaatg cagaacttga taaacacagg
     1201 ggacagaatt ccgcccatcc ggatatcagc cagtgcaatc agaagatctg cgagaatccg
     1261 aagactcacc ttggattt
//
LOCUS       DAOQGJ010001493         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86473_length_1277_cov_4.174304, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001493 DAOQGJ010000000
VERSION     DAOQGJ010001493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86473_length_1277_cov_4.174304"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PK683_19025"
     CDS             <1..1041
                     /locus_tag="PK683_19025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH10603.1"
                     /translation="LSEEHLPAQTENLKTLGFSAEHLLTLVNDILDYNKIEAGKVVLE
                     SIELNLPMLVSNIRQSMTARANELSNELTLILDENIPETIVGDPFRLSQILTNLISNA
                     VKFTSGGRVDLRVGVSEIMKNKARIFFSVEDTGIGIPKEKIESIFLGFTQADSSTARK
                     FGGTGLGLAITKKLLELFKSEIHLESEPGKGTKVFFEILFETRTKNKERTQNKDQGTT
                     MEFSDKKILIIDDYIVNLNIAKKFLAKIGIEPDLAAAGPDALEMLKEKDYDLIFLDLQ
                     MPEMDGFEVCRRIRKLNERYAEIPIIALTAAFVSEVQDRIIEAGMNDILAKPFKPEEL
                     HSIVFKWTGKSS"
     gene            complement(1033..>1277)
                     /locus_tag="PK683_19030"
     CDS             complement(1033..>1277)
                     /locus_tag="PK683_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10604.1"
                     /translation="SSASYYSYFHSGKEEEKVKPRTGIEALKFLLDFYDSSVWVNPDD
                     ERYWTSTTCEAIWDNVPMFFLSVDGISRAMKKLMSG"
BASE COUNT          415 a          243 c          275 g          344 t
ORIGIN      
        1 ctttcggaag aacatcttcc cgcacagact gaaaacctga aaactctcgg attttcagcg
       61 gaacaccttc tgactcttgt gaatgacata cttgattaca acaaaattga agcaggaaag
      121 gttgtgctcg aatccattga gctgaatctt ccgatgctcg tatcaaacat cagacagtcg
      181 atgactgcaa gagcaaatga gctttccaat gaactgactc ttatacttga tgagaacata
      241 cctgaaacga ttgtaggaga tccgttcaga ctttctcaaa tactgaccaa tcttatatcc
      301 aatgcagtta aatttacttc cgggggcagg gttgatctgc gagtgggtgt ttcagaaata
      361 atgaaaaata aggcaagaat atttttttct gtggaagata ccgggatagg aatcccgaag
      421 gagaaaatag agtctatttt ccttggattc actcaggcag attcctccac tgccagaaag
      481 ttcggaggta ccggactggg tcttgccatt acaaaaaaac ttctggaact tttcaaaagt
      541 gaaattcatc tggaaagtga accgggaaaa ggaactaaag ttttttttga aattcttttt
      601 gaaaccagga caaaaaacaa agaaagaaca cagaacaagg atcaggggac aactatggaa
      661 ttctcggata aaaaaattct gattatagat gattatatag tcaatttaaa tatcgcaaag
      721 aaattcctgg caaaaatcgg aatagagccg gatcttgcag cagcgggacc ggatgctctg
      781 gaaatgctga aagagaagga ctatgatctg atttttcttg atctgcagat gcctgaaatg
      841 gacggatttg aggtatgcag aagaattcga aaactgaatg aaaggtatgc agaaatcccg
      901 atcattgcac tgactgctgc atttgtctct gaggttcagg acaggatcat tgaggcaggc
      961 atgaatgata ttctggcgaa acctttcaag ccggaagaac tgcacagtat cgtttttaaa
     1021 tggacaggga aatcatcctg acatcagttt tttcattgct ctggatattc cgtctacact
     1081 cagaaaaaac atagggacat tgtcccagat ggcttcgcag gtagttgatg tccagtatct
     1141 ttcatcatcc ggatttaccc agacagatga gtcataaaaa tccagaagaa atttcagtgc
     1201 ctcaatgcct gttctcggct tgactttctc ttcctctttt cctgaatgaa aataggaata
     1261 ataagatgca gaacttc
//
LOCUS       DAOQGJ010001494         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86504_length_1277_cov_3.009820, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001494 DAOQGJ010000000
VERSION     DAOQGJ010001494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86504_length_1277_cov_3.009820"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PK683_19035"
     CDS             complement(<1..947)
                     /locus_tag="PK683_19035"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNH10605.1"
                     /translation="MKQIIYLLIIICFSDSCRNTDKNFFRRNAAMLAASVIASQGCSP
                     FEWSLPAGVPVPKIPSDNCMTSAKVELGRHLFYDKRLSANETMSCASCHRQNLAFSDG
                     RAVPKGISPPADTLPRNSQHLTNVAYSSKLTWINPNLSSLEVQVRVPLFADSPKEMGL
                     STNAYLDKLYTQPYYRDLFRDAFGTGAEKFSEQNVRYAIASFQRNLLSFNSPYDRYTR
                     KEASISSSASAGKEIFFGENAKCSKCHGGLNFTDAYEDVSTAFAETVYHDNGIHSIAY
                     YSSLSPDKRGLYNVTENPADIGKFKAPSLRNVALTFPYL"
     gene            complement(959..>1277)
                     /locus_tag="PK683_19040"
     CDS             complement(959..>1277)
                     /locus_tag="PK683_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10606.1"
                     /translation="EKEPDLMNLGRERISFSEKDRGKFKTPTLRNIALTAPYMHDGRF
                     RTLVEVIEHFSSGVKKSPWTDKELLNPANRLNEEQKKELISFLETLTDREITEDRRLS
                     SPF"
BASE COUNT          336 a          266 c          284 g          391 t
ORIGIN      
        1 tgcaagtaag gaaaagtaag tgcgacgttc ctcagactgg gagccttgaa ttttccgata
       61 tctgcgggat tttcagtgac attgtacagc cctcttttat ccggactcag actgctgtaa
      121 taggcaatag aatgtattcc gttatcatga tagacagttt cagcaaaggc agtactgacg
      181 tcttcatacg catctgtaaa attcagccca ccgtggcatt ttgagcattt tgcattttct
      241 ccaaaaaaaa tttcctttcc ggctgaggca cttgaagaaa tagatgcctc cttccttgtg
      301 tatctgtcat atggtgaatt gaaactcaga agatttctct gaaaagatgc aattgcataa
      361 cggacattct gttctgaaaa cttttcagca ccggtgccga atgcatcccg aaaaagatcc
      421 ctgtaataag gctgagtgta aagtttgtcc agatacgcat ttgtggaaag acccatttct
      481 ttcggtgagt cagcgaacag agggactctc acctgaactt ccagtgagga aagatttggg
      541 ttgatccagg ttagctttga gctgtatgcg acatttgtca gatgctgtga gtttctaggc
      601 agtgtgtctg caggaggaga gattcctttc ggaacggcgc gtccgtcaga aaaggcaaga
      661 ttctgcctat ggcaggatgc acaggacatt gtttcatttg cagagagtct cttatcgtag
      721 aaaagatgtc ttcccagttc cactttggca gaagtcatac agttgtcaga cgggattttc
      781 ggaacaggaa cccctgcagg aagactccac tcaaaagggg agcatccctg tgaagcaatg
      841 accgatgcag caagcattgc tgcattccgt ctgaaaaaat ttttatctgt atttctgcat
      901 gaatcggaaa aacagatgat tataagtaaa tatataattt gtttcattct ttttctgctt
      961 aaaacggact ggaaagcctt ctgtcttcag taatttccct gtctgtcaaa gtttccaaaa
     1021 aagagatcag ttcttttttc tgttcctcat tcagtctgtt tgccggattc agaagctctt
     1081 tgtctgtcca tgggcttttt ttgactcctg aagaaaaatg ttcaatcact tcgacaagcg
     1141 tcctgaatct tccatcgtgc atgtacggag cagtcagggc gatatttctg agcgtgggcg
     1201 ttttaaattt tcccctgtct ttttcagaga aggaaattct ttcccgtcca agattcatca
     1261 ggtcaggttc tttttcg
//
LOCUS       DAOQGJ010001495         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86536_length_1277_cov_2.265957, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001495 DAOQGJ010000000
VERSION     DAOQGJ010001495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86536_length_1277_cov_2.265957"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..689)
                     /locus_tag="PK683_19045"
     CDS             complement(153..689)
                     /locus_tag="PK683_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNH10607.1"
                     /translation="MDIKEPKDIDGIHQADNPMPEWWKLIWLLTIVFSIAYAVYFHGF
                     SKWSQHEAIVIEVAEHEKAFPAKVAALPVSNDGSNPLRDNKEAIAEGQKTFGSVCAAC
                     HKADAKGLVGPDLTDAAWIHGDTDAAIFEVIMKGVGPEKAKLGKGPMPPHERLGQDKV
                     YQIMAWLASNNATLKKGK"
     gene            complement(692..835)
                     /locus_tag="PK683_19050"
     CDS             complement(692..835)
                     /locus_tag="PK683_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNH10608.1"
                     /translation="MDMDWLVLYKVARLPLLFIAMIVITVYVYGRSKESIERPKYRMM
                     EED"
     gene            complement(836..>1277)
                     /locus_tag="PK683_19055"
     CDS             complement(836..>1277)
                     /locus_tag="PK683_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HNH10609.1"
                     /translation="KWEVDRFDPNKVYGKYGYSKGGEYVYDHPFLWGSKRTGPDLAHE
                     SQIQPSPEWQKRHLMNPRETSPGSVMPAYPWLFNEKNLVNADDIVSHMKGLSKVGVPY
                     TDADYSAAKGLIAGKTEGDALVAYLMKLGKDYAEFEKSRKAEGK"
BASE COUNT          328 a          317 c          244 g          388 t
ORIGIN      
        1 agaatttcct gtgcggaatg tccagtcgga tcaacggctg atcagtattg atcactatcc
       61 aaggacagat tatgaaaatc agcagaagca ctgcttccac tgcgtatctg agatttctga
      121 atttcccttc tatatgcctg gcgataatca ttttattttc cttttttcaa agtggcatta
      181 tttgaagcaa gccatgccat aatctgataa actttatcct gtccgagtct ttcatgagga
      241 ggcatagggc cttttcccag tttagccttt tcaggaccta ctcccttcat gatcacttcg
      301 aagatagctg catctgtatc cccatgaatc caggctgcat ctgtcagatc aggtccgacc
      361 agaccctttg catcagcttt gtgacatgcg gcacagactg atccaaaagt cttctgaccc
      421 tctgcaattg cttccttgtt gtctcggaga ggattgcttc cgtcgttcga aacaggaaga
      481 gccgcaacct ttgcaggaaa cgccttttca tgttcagcaa cttcaatcac aattgcttca
      541 tgctgactcc acttggaaaa accgtgaaag tacactgcat aagcaattga aaaaactatt
      601 gtaagaagcc agatcagctt ccaccattca ggcatcggat tgtcggcctg atgtattcca
      661 tcgatatcct taggttcttt aatatccata ttcaatcctc ctccatcatt cggtatttgg
      721 gtctttcaat agattctttg cttcttccgt aaacgtagac tgtaatcaca atcatagcta
      781 taaagagaag cggaagacga gcaactttgt aaagtacaag ccaatccata tccatttact
      841 taccctctgc ctttctgctc ttttcgaatt ctgcgtagtc ttttccaagc ttcatcagat
      901 aagcaaccaa agcatcgcct tcagtctttc cggcaatcag tcccttcgca gccgaatagt
      961 cggcatcggt ataaggaact cctactttgg agagaccttt catatgactg acgatatcgt
     1021 ctgcatttac caggttcttt tcattgaaaa gccaagggta tgcaggcatt acagatccgg
     1081 gagatgtctc tctaggattc atcaggtgtc ttttctgcca ttctggtgaa ggctgaatct
     1141 gggattcgtg agccagatcc ggaccggttc tttttgaacc ccaaaggaaa ggatgatcat
     1201 atacatattc tccgcccttg gaatacccat attttccgta aaccttgttc ggatcaaatc
     1261 tgtccacttc ccatttg
//
LOCUS       DAOQGJ010001496         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86548_length_1277_cov_2.063830, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001496 DAOQGJ010000000
VERSION     DAOQGJ010001496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86548_length_1277_cov_2.063830"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK683_19060"
     CDS             complement(<1..396)
                     /locus_tag="PK683_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10610.1"
                     /translation="MQIEREDKQRLFWVVVSAFFFAMCLFGFYIFRGDPGSDVSYTRL
                     ANLKSVIDKGVIRTQTEAFGNFILYFNKKVFGLKVLNAYHITLALIFSFTLHFAMLNF
                     YRKEWRLNHFFLIYLVGFVPFSINLPYFYM"
     gene            485..>1277
                     /locus_tag="PK683_19065"
     CDS             485..>1277
                     /locus_tag="PK683_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH10611.1"
                     /translation="MAESKDHVLYGENKEPAKDKPWIFRTYAGHTNPKSSNELFRKNL
                     SKGQTGLSIAFDLPTQCGYSSDHSIARPEVGKVGVPINSLEDFRVLFDQIPIGEMNTS
                     MTINATSMWLLSLYIALAEENSVSSDKLEGTTQNDIIKEYLARGTYIFPPAQSIRMIV
                     DMYEYCLRNVPKWNPSNICSYHLQEAGATPVQELAFALATAIAILDAIKERNCFNADE
                     FEKCVGRISFFVNAGMRFVEEMCKMRAFTEMWEEITRDRYGVKNPK"
BASE COUNT          410 a          269 c          271 g          327 t
ORIGIN      
        1 catataaaaa taaggaagat tgatcgaaaa aggaacaaat cctaccagat aaatcaggaa
       61 gaaatggttc agtctccact cctttctgta aaaattgagc attgcaaagt gcagagtgaa
      121 actgaatatt aaagcgagag taatatgata tgcattcagt accttcagtc cgaatacctt
      181 cttgttgaaa tagagaataa aattgccgaa agcttctgtc tgagtcctga tgactccctt
      241 gtcgatgaca gacttgagat tggcgagtct tgtgtacgaa acatcgctgc cgggatcgcc
      301 tctgaatata taaaaaccaa agaggcacat cgcaaaaaag aaggcggata ccaccaccca
      361 aaataatctc tgtttgtctt ccctttcaat ctgcatgagg atttcttgct atactttttc
      421 actgccataa taaaatctgg gcttaatttt atatagaaaa tttttattta aggggaagaa
      481 aactatggca gagtcaaaag accacgtcct ctacggcgaa aacaaagagc cagccaaaga
      541 caagccttgg attttcagaa cttacgcagg acatacgaac ccaaaatcgt ccaacgaact
      601 tttccgaaaa aacctctcaa aaggacagac tggactttcc attgcattcg atctgcctac
      661 tcagtgcgga tacagctccg accacagcat tgcaaggcct gaagtgggca aggtaggtgt
      721 tcctatcaat tcactcgaag atttcagagt tctttttgac cagattccta tcggggaaat
      781 gaacacttcg atgacaatta atgccacatc tatgtggctc ctgtctctgt acattgctct
      841 ggcagaggaa aacagtgttt cttccgacaa gctggaaggg acaacccaga atgacattat
      901 caaggaatac ctggcaagag gcacatatat tttccctcct gcacagtcga tccgtatgat
      961 agtggatatg tatgaatact gtctgagaaa tgttcccaaa tggaatcctt ccaatatctg
     1021 ctcctaccat cttcaggaag ccggtgcgac tcctgtgcag gaacttgcgt ttgctctggc
     1081 aacggcaatt gccattctgg atgcaatcaa ggaaagaaac tgtttcaatg cagatgagtt
     1141 cgaaaaatgc gtgggcagga tctccttttt tgtaaatgca ggaatgagat ttgtggaaga
     1201 gatgtgcaag atgcgggctt tcactgaaat gtgggaagaa atcaccagag acagatacgg
     1261 agtaaaaaac cctaaat
//
LOCUS       DAOQGJ010001497         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86597_length_1276_cov_3.349713, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001497 DAOQGJ010000000
VERSION     DAOQGJ010001497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86597_length_1276_cov_3.349713"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PK683_19070"
     CDS             <1..826
                     /locus_tag="PK683_19070"
                     /inference="COORDINATES: protein motif:HMM:NF017142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HNH10612.1"
                     /translation="TSFYDNYILYKIGIMSKNDWLNEVLSDINRLADSSGEDYMSLEE
                     ASFTAWTKYYRQNPNSHNVSISYYIKGFLLSLCMNISILEQTDGRKNLMHLMRTLYRK
                     YHLKKNKGFTKQEFFRTAEEEFGFDLQAEFEQYISEKKRIPAEFYLDKLGIKLAKTMK
                     KSVFPFEVKTESGKQIISKIFSQWSIPDLEIGDELIGINSRRVSRDVFEKLKLSLPDS
                     SEAELILSRKGRIFYAKVETKMIYTYQIPYPVLEEDFEEIFRTNRIAECFFRREAL"
     gene            826..>1276
                     /locus_tag="PK683_19075"
     CDS             826..>1276
                     /locus_tag="PK683_19075"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNH10613.1"
                     /translation="MLGTSWILNTNGKTEIDSDEALPVFRVKTWIHLTPKNEEELTKL
                     LKNYNIHPLTIEDLMNLHSRIKKEEFPNYTFVIFRGIHFDANIISAKNFNFIITKNVL
                     ITVALDHRNTINDIIENWQNGKDLLDRGLEFIIHRIMDAETDHLLPIV"
BASE COUNT          448 a          249 c          248 g          331 t
ORIGIN      
        1 cacaagcttt tatgataatt atatcttata taaaattgga atcatgtcca aaaatgactg
       61 gctgaatgaa gttctctccg atatcaaccg gcttgcagat tcttcaggcg aagactatat
      121 gagtctggaa gaagcttcct ttacagcatg gacaaagtat taccgacaga atcccaattc
      181 gcacaatgtc tccatttcct actacataaa gggatttctt ctctctcttt gcatgaacat
      241 cagcatactg gaacagacag acggaagaaa aaacctgatg catcttatga gaactcttta
      301 cagaaaatat catctgaaaa aaaacaaggg attcaccaaa caggaattct tcagaacggc
      361 agaggaagaa ttcgggtttg atcttcaggc ggaatttgaa cagtatatat ccgaaaagaa
      421 aagaatccct gccgaatttt atctggacaa gctgggaatc aaactagcaa aaacaatgaa
      481 aaaaagtgtc tttccttttg aagtgaagac tgaatcagga aagcagatta tttccaaaat
      541 attcagccaa tggagtattc ccgatctgga aatcggagat gaactgatag gaatcaactc
      601 gagaagggtc agcagggatg tattcgagaa actgaaactc tctcttccgg acagttcgga
      661 agctgaactg attctttcga gaaagggaag aattttttat gcaaaggtgg aaacaaaaat
      721 gatttataca taccagatcc cttatcctgt cctggaagaa gactttgagg aaatattccg
      781 cacaaacaga atcgctgaat gctttttcag aagagaggct ctctgatgct tggaacatca
      841 tggatactga acacaaacgg aaagactgaa attgattctg acgaggctct ccctgtattc
      901 agggtaaaaa cctggataca tcttactccc aagaacgagg aagagctgac aaaacttttg
      961 aaaaactata atatacatcc acttactata gaggatctta tgaatcttca cagccggatc
     1021 aagaaggagg aattccccaa ttatactttc gtaattttca gaggaattca tttcgatgca
     1081 aacatcattt ctgcaaaaaa cttcaacttc atcattacaa aaaatgtgct gatcacagtt
     1141 gcacttgacc accgaaacac aatcaatgac atcattgaaa actggcagaa cggaaaggat
     1201 cttctggaca ggggtcttga attcatcatt cacaggatca tggatgcgga aacggatcat
     1261 ttacttccga ttgtat
//
LOCUS       DAOQGJ010001498         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86601_length_1276_cov_3.275184, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001498 DAOQGJ010000000
VERSION     DAOQGJ010001498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86601_length_1276_cov_3.275184"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PK683_19080"
     CDS             <1..439
                     /locus_tag="PK683_19080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH10614.1"
                     /translation="VDFEAGESWPDILNQAGFDAKKSSVTVSTGVSMYLTQEANLALL
                     KQTAGLAKGSVFAMTFMLSLNLLGPEERSVMEFVMKKAGEAGTPFLSLFSPPEILELA
                     KEAGFSKSQYVSGEDLFQRYFADRTDGLRAGIAEAFLVAESES"
     gene            complement(530..982)
                     /locus_tag="PK683_19085"
     CDS             complement(530..982)
                     /locus_tag="PK683_19085"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH10615.1"
                     /translation="MKKEKNRLRSHCPVNFGLEAFGDRWALLILRDIIFRGKRTYGEF
                     LKSEEGFATNILGSRLDHLTEEGILQRDKDESDGRKDIFSLTEKGLDLIPLIFEMVLW
                     SSKYDSESEAKRILPLVELIKKDNRKISSKVREKVKRGEGIVRDYLTK"
     gene            1065..>1276
                     /locus_tag="PK683_19090"
     CDS             1065..>1276
                     /locus_tag="PK683_19090"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH10616.1"
                     /translation="MIDIIHRIGIKTPAEKVYKALTTLEGLGSWWTEEVSGETKTGGR
                     IEFLFRSKTGELIGKMVMEVKELNDQ"
BASE COUNT          352 a          266 c          279 g          379 t
ORIGIN      
        1 ggtggacttt gaggcaggcg aatcctggcc ggatattctg aatcaggctg gatttgatgc
       61 aaagaaatct tctgtcacag tttcgaccgg agtctctatg tatcttaccc aggaggcaaa
      121 tcttgctctt ctgaaacaga cagcagggct tgcgaaaggt tctgtttttg caatgacgtt
      181 catgctttct ctgaatctgc ttggacctga agaaagatcc gttatggaat ttgtaatgaa
      241 aaaagcaggc gaggcaggta cacctttcct gagtctattt tcacctcccg aaatactgga
      301 gctggcaaaa gaggccggtt tcagcaagtc gcagtatgtg tccggtgaag atctgtttca
      361 aagatatttc gcagaccgca cagacgggct cagagccgga attgcagaag cattccttgt
      421 tgcagaatca gaatcctgaa cagcagttca ggatatctct tttcttttgc atgaatcagc
      481 agagtgcatc tttctgcggt aagctcagtc ttggctgcac cggacctctt tactttgtca
      541 gataatcacg cacaattcct tcaccgcgtt ttactttttc tctcacttta ctgctgatct
      601 ttcggttgtc tttttttatg agttcaacaa ggggcagaat gcgtttcgcc tcggattcgc
      661 tgtcatattt ggaactccag agaaccattt caaagatcaa aggaatcaga tcgagaccct
      721 tttcagtcag tgagaaaata tcctttcttc cgtcagactc atctttatca cgctgaagta
      781 tgccttcttc agtgagatga tccagacggg atccaagaat atttgtagca aaaccttctt
      841 ctgatttcag gaattctcca tacgttctct ttcctcgaaa tataatatcc cgaagaatca
      901 gcagtgccca tctgtctccg aaagcctcca gtccgaaatt tacaggacag tgtgaacgaa
      961 gtctgttctt ttcttttttc atattctgca ttagtattcg gaaatttact tgcaatttgc
     1021 aagtaaattt tttaaactgt aaaaaaacaa ttttggagat tcatatgatt gacatcattc
     1081 acagaatagg tattaaaact cctgcagaga aagtctataa ggctctgaca actttagaag
     1141 gtctaggcag ttggtggact gaggaagtaa gcggtgagac aaagaccgga ggaagaatcg
     1201 aatttttatt ccgctctaaa accggagagc tcatcggtaa aatggttatg gaggtgaagg
     1261 aactgaatga tcagaa
//
LOCUS       DAOQGJ010001499         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86742_length_1275_cov_3.348361, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001499 DAOQGJ010000000
VERSION     DAOQGJ010001499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86742_length_1275_cov_3.348361"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK683_19095"
     CDS             <1..386
                     /locus_tag="PK683_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10617.1"
                     /translation="LRLFIFAGAVFTVFPVLAGTGADAEIFDPPTNIRTSDGKVLCRV
                     TKVKTIFILARASKGKKFTGWYLTDACSPGEKPGTVWGMVHKSQIRIKSKRSDLFPVD
                     PKKFIQCAENPSNVPESCAAENQAK"
     gene            complement(402..902)
                     /locus_tag="PK683_19100"
     CDS             complement(402..902)
                     /locus_tag="PK683_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNH10618.1"
                     /translation="MAQDPSFDIISKIEKSELANAISLAMTEITTRFDFKGSKSDIKH
                     EGDNLVITSDNDIKIKQVIDVLTNKLLKRGIGIKAFEFEKAKLEQATGGTVRMKVKVQ
                     NGLEKEHTKEITRILKDSKLKIQPTIMGEYVRVTGKKKDDLQEAQKLLKNADLNFDFT
                     FTNYKS"
     gene            complement(880..>1275)
                     /locus_tag="PK683_19105"
     CDS             complement(880..>1275)
                     /locus_tag="PK683_19105"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNH10619.1"
                     /translation="ESSNLLQVQDRLISESEKIGNFVLIQYRTSGEKIENLKELGDSL
                     KNRVPESLTVLLNEDKAAPSTLILMAGRKAVENKIQCNDLLKKILPLLDGRGGGKPDL
                     AQGSVKSLENLNKAVEVLKEALNGSGSVV"
BASE COUNT          311 a          276 c          263 g          425 t
ORIGIN      
        1 ttctaagatt atttattttt gccggagcgg ttttcacagt atttcctgta ttggcaggga
       61 caggcgcaga tgcggagata tttgacccgc cgacaaatat acggacctca gatggaaaag
      121 tcctctgcag ggtgaccaag gtgaaaacaa tttttattct tgcaagggca tcaaaaggaa
      181 aaaaatttac cggatggtat cttactgatg cctgcagtcc cggcgaaaaa cctgggactg
      241 tctggggtat ggttcacaaa tcacagattc ggatcaaatc taaaagatcg gatctgtttc
      301 cagtcgatcc gaagaaattc attcagtgcg ctgaaaatcc atccaatgtc cctgaatcct
      361 gtgctgctga gaatcaggcg aaatgaattt cagctgcttt ctcaggattt gtaatttgta
      421 aaagtaaagt cgaaattcag atctgcattc ttcaggagtt tctgagcttc ctgaagatca
      481 tcttttttct ttcctgtcac tcttacatat tctcccatga tggtaggctg gattttcagc
      541 ttgctgtctt tcagaattct tgtgatttcc ttggtgtgct ctttttcaag accgttctgc
      601 accttgactt tcattcggac agttccgcct gtggcctgtt ccagcttggc tttttcaaat
      661 tcaaaagctt tgattccgat ccctcttttc agaagtttgt tcgtcagcac atctatgacc
      721 tgtttgatct tgatatcatt gtctgatgtg attacaagat tgtcgccttc gtgcttgata
      781 tctgactttg agcctttgaa atcaaacctt gtggtaattt cagtcattgc aagtgagatt
      841 gcattcgcaa gttcagactt ttctattttg gaaataatgt caaacgacgg atcctgagcc
      901 atttaaagcc tcctttaaaa cttcaactgc cttgttcaga ttctcaagcg acttgactga
      961 accctgcgcg aggtcgggtt ttccgcctcc gcgtccgtca agaagaggga gaatcttttt
     1021 caataaatca ttacactgta ttttattttc aactgctttt ctgcctgcca tgagaatcag
     1081 agtcgaagga gcagccttgt cttcgttcag aagaactgta agtgattcag gcaccctgtt
     1141 tttcagactg tctccaagtt ctttcagatt ctcgattttt tcgcctgagg ttctgtactg
     1201 gatcagaaca aagtttccta ttttttccga ttctgaaatc agtctgtcct ggacctgaag
     1261 aaggtttgaa ctttc
//
LOCUS       DAOQGJ010001500         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_86894_length_1274_cov_2.646432, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001500 DAOQGJ010000000
VERSION     DAOQGJ010001500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86894_length_1274_cov_2.646432"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PK683_19110"
     CDS             complement(<1..443)
                     /locus_tag="PK683_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10620.1"
                     /translation="MIKYFVLLTLLSLNSFCFLQNANGEQKKKDKDTQNLILAAAVLT
                     ACVPGSNGFYMLNFETGNYECVRASLKYSNSDIDIYVQDGLESMQTEYGINKISYSSI
                     ASGFSTNIMPKLKPAIGASTDINGDKKITVMFFVSSFEVSSGTYVA"
     gene            complement(440..>1274)
                     /locus_tag="PK683_19115"
     CDS             complement(440..>1274)
                     /locus_tag="PK683_19115"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH10621.1"
                     /translation="RLQTGNLEEISAAVEEAKISVETITESLKQGQSDLDKITDEIKS
                     LADSNEKTSSEISSSSRIMNRASENAVSGEDHLKEMGNSMSKIESSYNRMIQIMEGIH
                     DIADRINLLSLNASIEAARAGDFGKGFSVVASEVSKLADQTSGNLKESDKLIKSIKTD
                     IREANEKLQSGIRLFMDIAQEVIRVNETFKSVSVQADMENRKLEKFRSGISDYSENVK
                     LLGSALSEQKYTMEAITESVDLVSRSSQDILKDLEKLQNAADESKIISENIVKILGEK
                     S"
BASE COUNT          311 a          289 c          244 g          430 t
ORIGIN      
        1 gccacgtatg ttcctgaact gacttcaaaa ctggaaacaa agaacattac ggtaattttc
       61 ttatccccgt taatgtcagt tgatgctccg atcgcgggtt taagcttcgg cataatattc
      121 gtactgaatc ctgatgcaat ggaagaatag ctgattttat ttatgccgta ttcagtctgc
      181 attgattcaa gtccgtcctg cacatagatg tcaatgtccg aatttgaata tttcagagat
      241 gctctcacgc attcataatt ccctgtttcg aaattcagca tatagaatcc gtttgaaccc
      301 gggacacatg cagtgagcac tgccgcagca agaatcagat tctgggtgtc cttatccttt
      361 tttttctgtt caccgtttgc gttctgcaga aaacagaatg aattcagtga cagaagcgtc
      421 agcagtacaa aatattttat catgattttt ctcccagaat ttttacaatg ttttccgata
      481 tgatttttga ttcatccgct gcattttgaa gtttttccag atctttcagt atgtcctgtg
      541 acgatctgct gaccagatct accgattccg tgattgcttc cattgtgtat ttctgctcgg
      601 agagggctga acccagaagc ttcacattct ccgaataatc actgattccg gaacggaact
      661 tttccagttt tctgttttcc atatccgcct gaacagacac gctcttgaaa gtctcattga
      721 ctcgaatgac ttcctgtgcg atatccatga agagtctgat gccgctctga agcttttcat
      781 tcgcttctct gatatcagtc ttgattgatt tgatcagctt gtcgctttcc ttcagattgc
      841 ctgaggtctg atctgcgagc ttggagactt ctgaggcaac cacggaaaac ccttttccaa
      901 aatcacctgc ccttgctgcc tcaatggacg cattcagaga cagaaggttt attctgtccg
      961 caatatcatg gattccttcc ataatctgaa tcattctgtt gtacgaagat tcaattttag
     1021 acatggaatt tcccatttcc ttcagatgat cttcacctga aactgcattc tcgctggctc
     1081 tgttcataat tctcgatgag gagctgattt cactggatgt cttttcattt gaatctgcaa
     1141 ggcttttaat ttcatcagtg attttatcaa gatcggactg accctgcttg agactttcag
     1201 tgatggtttc aacggatatt ttggcttctt caacagcagc agaaatttct tccagattgc
     1261 ctgtctgaag tctt
//
LOCUS       DAOQGJ010001501         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87010_length_1273_cov_2.488506, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001501 DAOQGJ010000000
VERSION     DAOQGJ010001501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87010_length_1273_cov_2.488506"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PK683_19120"
     CDS             complement(<1..328)
                     /locus_tag="PK683_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10622.1"
                     /translation="MNSKKKFPGIQILLIVFSAVQYYGCFSWNVKETGPKEPEAVEDA
                     VPPAGYAPVFMKKTEPLSEEKPESAETVVTRTDTRNERKVRAYVHILDKEGTYLSGGT
                     DKKRKNR"
     gene            complement(444..1103)
                     /locus_tag="PK683_19125"
     CDS             complement(444..1103)
                     /locus_tag="PK683_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10623.1"
                     /translation="MKKIILSHIIFTLALTADTITLRSGLIEKNVKTNLRKNSVMVMY
                     ESGKTKEISKNELKAIQKSDVVWKKNPPIKTVPVPAKTPSKNPAPSETAEKSGENTYL
                     CSLHTFSGGSDAPPEDKRIYKKSFEKKSLLFLHTEAVCFTEVRKPNSSVKLFFRYLDS
                     AGKPVSESSYETEVGPDMGSFSYITGYGYEESDSWEQGRYTVEFYLDDELLETADFTV
                     R"
BASE COUNT          315 a          261 c          243 g          454 t
ORIGIN      
        1 gtctgttttt tctttttttg tcagttcctc cggaaagata ggtaccttct ttgtccagaa
       61 tatgcacata ggctctgact tttctttcat ttcgggtgtc ggttcttgtg acaactgtct
      121 ctgcggattc cggtttttct tcagaaagag gttctgtctt cttcataaat acaggcgcgt
      181 atcctgcagg cgggacagca tcctcaactg cttcgggctc tttcggaccg gtttctttga
      241 cattccagct gaagcagcca tagtactgca ctgcagaaaa gacaatcaga agaatctgaa
      301 tccctggaaa ttttttttta ctgttcatat ttgtttctcc atatttcaga actgacttct
      361 tagtcagttc tgtgctgctt gttttttatt aatttcatta taaaatattt tacaactttc
      421 aataagtcct ccgttcagaa aaatcatctg actgtgaaat ctgctgtttc cagaagttca
      481 tcgtccagat aaaattccac agtgtatctg ccctgttccc aggaatcgga ctcttcatat
      541 ccgtacccgg ttatgtaaga aaaacttccc atatcaggac cgacttccgt ttcgtaactg
      601 ctttctgaga cgggctttcc tgccgaatca agatacctga aaaaaagttt taccgaggaa
      661 ttcggttttc tgacttcggt gaagcagact gcctcagtgt gaagaaacag aagagatttt
      721 ttttcaaaag actttttata aattctttta tcttccggag gagcatctga gcctccggaa
      781 aaggtatgga ggctgcagag ataggtgttt tcccctgatt tttctgcagt ttcagagggt
      841 gcaggatttt tcgaaggcgt tttcgcaggc acggggaccg tttttatggg aggatttttt
      901 ttccagacaa catccgactt ctgaattgct ttcagttcgt tcttggatat ttcttttgtt
      961 tttccgcttt catacatcac cataactgaa tttttcctca aatttgtttt cacatttttt
     1021 tcgatcagac cgcttctgag ggtgattgta tccgcagtca atgcgagggt gaaaattata
     1081 tgagacagga ttattttctt cattatatat ttccgtgtac tgtaatctgt ccagaatatc
     1141 ggcagaaaaa cttcagaacc tatactgatc ttttcatgaa aatcagatac tgcacattta
     1201 tgtcgaaggt tctccctgtg agattttcct tctgtaaata aattttcaga taatataaat
     1261 tttctattta cag
//
LOCUS       DAOQGJ010001502         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87104_length_1272_cov_3.071487, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001502 DAOQGJ010000000
VERSION     DAOQGJ010001502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87104_length_1272_cov_3.071487"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PK683_19130"
     CDS             <1..806
                     /locus_tag="PK683_19130"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH10624.1"
                     /translation="DVWTFDLRGKKEADDSSWFFGKRTYNYSFDEHCRYDADTALNFI
                     LKASGTDKVNWIGHSMGGMVMYMRIGTMNENRVANLVTLGSPFLFPFPSWKLKIQKKA
                     GFFTPLFWVIPSGFFSRIMGYTGLPMMNEQYLSSMYWYPPNLGEENEKELFKYGIDDE
                     SPKLLAQFAESLGEWSYRSKDGKTDFTENLRNIHIPALLVAGRRDHMGSPYQVRYVYD
                     RISSRDRTMMVIGRTEGKSEDYGHVDLVISKDSVKDIQMPIIEWLNRRN"
     gene            811..>1272
                     /locus_tag="PK683_19135"
     CDS             811..>1272
                     /locus_tag="PK683_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10625.1"
                     /translation="MKINSFSQSAIINKFINSGVPSFFSHLLRISFVLSFWACSCTSL
                     PVYKKKDLEKKFILLEGRKQHRLYLFDRTSSDSRKAVLLADPYFLSEESLYLQDSYSY
                     GMEWKDSYRRSGIPGKQIVKNSSENGNLISYLNKKGYIVFLLKYNEETGLTD"
BASE COUNT          389 a          247 c          282 g          354 t
ORIGIN      
        1 atgatgtctg gacatttgat cttcgcggaa aaaaagaagc agatgacagc tcatggtttt
       61 ttggtaagag aacttataat tacagctttg atgaacactg cagatacgat gctgatacgg
      121 cactgaactt tattctcaag gcttccggaa cagataaagt caactggatc ggacacagta
      181 tgggcggaat ggtcatgtac atgagaatcg gaacgatgaa tgaaaacagg gttgcaaatc
      241 ttgtcactct gggttctcct tttctgttcc ctttcccgtc atggaaactc aaaatccaga
      301 agaaggcagg tttttttaca cctcttttct gggtaatccc ttccggtttt ttttccagaa
      361 tcatgggata taccggtctg cctatgatga atgaacaata tctctcgtca atgtactggt
      421 accccccgaa tctaggtgaa gaaaatgaga aagagctgtt taaatacgga atagacgatg
      481 aaagtcctaa gctgcttgct caatttgcgg aatctctcgg agaatggagt tacagatcta
      541 aggacggaaa gactgatttt actgaaaacc tcagaaatat tcatattccc gcacttcttg
      601 tagcaggaag acgggatcat atgggttcgc cctaccaagt gcgttatgta tatgacagga
      661 tctcaagcag agacaggaca atgatggtca taggcaggac ggagggcaaa tctgaggatt
      721 acgggcatgt ggatctggtc atttcaaaag actctgtaaa ggacatccag atgccgatca
      781 ttgaatggct gaacaggaga aattaagacc ttgaaaatca actcattcag tcagtctgca
      841 attataaata aatttattaa ctccggagtt ccatcgtttt tttctcatct gctgcggatc
      901 agttttgttc tctccttctg ggcatgttcc tgcacctcgc ttcccgtata caagaagaag
      961 gatctggaaa aaaaattcat tcttctggag ggcagaaaac aacacagact gtaccttttt
     1021 gacaggactt catcagacag cagaaaggct gttctgcttg cagatccata tttcctttct
     1081 gaagaaagcc tgtatctgca ggactcttat tcttacggaa tggaatggaa ggattcctac
     1141 agacgttcag gaataccggg aaaacagatt gtaaagaact cctcagaaaa cgggaatctg
     1201 atttcatatt tgaacaaaaa gggatatatt gtatttcttt tgaaatataa tgaagaaact
     1261 ggactgaccg at
//
LOCUS       DAOQGJ010001503         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87329_length_1270_cov_3.409053, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001503 DAOQGJ010000000
VERSION     DAOQGJ010001503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87329_length_1270_cov_3.409053"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1270
                     /gene="kdpB"
                     /locus_tag="PK683_19140"
     CDS             <1..>1270
                     /gene="kdpB"
                     /locus_tag="PK683_19140"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNH10626.1"
                     /translation="RIVVRITAELGNSFLDKMIKLVEGASRGKTPNEIALEILLLVLS
                     AVFILAVVSLKPMSVYAESFSSSKSNVSMTNLIALLICLLPTTIGGLLSAIGISGIDR
                     LIQKNIISKSGKAVEAAGDIDVLLLDKTGTITFGNRMAVDFFPAEGVSDMELADAARI
                     ASIADETPEGRSVVVLAKEKFSLRAVDTNSMKISFVPFSAETRMSGADIEENGKTAVI
                     RKGAYDSVKKLTESMGGNFSQSVENSVNKISSLGGTPLAVCRNSEMLGVIYLKDVVKG
                     GIRERFAKLREMGIRTVMITGDNPRTAAVIAAEAGVDDFMAEATPESKLARIRHEQAM
                     GHLVAMTGDGTNDAPALAQADVGVAMNTGTQAAREAGNMVDLDSNPTKLIEIVETGKQ
                     LLMTRGALTAFSIANDIAKYFAILPAIFAGL"
BASE COUNT          382 a          240 c          332 g          316 t
ORIGIN      
        1 caggattgtt gtgaggatca ctgctgaact gggcaacagc tttctcgaca aaatgataaa
       61 acttgtagaa ggagccagca gaggaaaaac tccgaatgaa atcgcactgg agatacttct
      121 ccttgtgctc tctgctgtct ttattcttgc agtcgtttct ctgaaaccga tgtctgtgta
      181 tgcggaaagt ttttcctcct caaaatcaaa tgtatctatg acaaatctga tcgctcttct
      241 gatctgtctc ctgccgacta caattggagg tcttctgagt gcaatcggaa tcagcggaat
      301 tgacagactg attcagaaga atattatttc caaaagcggg aaggcagtgg aggctgcggg
      361 cgatatcgat gtgcttcttc tggataagac gggtacaata actttcggga accgaatggc
      421 agtggatttt tttcctgcag aaggagtgag tgatatggaa ctggcagatg cggcaaggat
      481 tgcctccatc gcagatgaga ctccggaagg aagatcagta gtcgtactgg caaaggaaaa
      541 attttcactg agagcagttg atacaaacag tatgaaaatt tcctttgtgc ctttcagtgc
      601 agaaacaaga atgagcggag cggacataga ggagaatgga aaaacagcag tcattcgtaa
      661 aggtgcttac gattcagtaa aaaagcttac agaaagtatg ggaggaaatt tttctcagtc
      721 agtggaaaat tcagtgaata agatctcttc acttggagga acacctctgg ctgtatgcag
      781 aaattctgaa atgctcggtg tgatttatct taaggacgtt gtgaagggcg gtattcggga
      841 gcgttttgca aaactcagag aaatgggaat ccggacagta atgattacag gagacaatcc
      901 tagaactgct gcagtcattg cggcagaggc aggcgtggat gattttatgg ctgaagcaac
      961 tcctgaatcc aaacttgcca gaatcagaca tgaacaggcg atgggtcatc tggtggcaat
     1021 gacaggagac ggaacaaacg atgctcctgc acttgctcag gctgatgtgg gtgtggcaat
     1081 gaacacaggg acccaggcgg caagagaggc agggaatatg gtggatctgg attcaaatcc
     1141 tacaaaactg attgaaatag ttgaaacagg aaaacagctt ctgatgacaa gaggagctct
     1201 gacagccttc agtatagcaa atgatatcgc aaaatacttc gcaattctcc ccgctatttt
     1261 tgcaggactt
//
LOCUS       DAOQGJ010001504         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87332_length_1270_cov_3.282305, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001504 DAOQGJ010000000
VERSION     DAOQGJ010001504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87332_length_1270_cov_3.282305"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PK683_19145"
     CDS             <1..412
                     /locus_tag="PK683_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10627.1"
                     /translation="SLSIQVDLFKTSHSSLRFNMLSGLALFFSSLLPLFLHKSFSEGL
                     VDAGLKAASFTYGPLLGLFLLARISKSHISYSQKNLNMALLLSLFSCIGFFLLFRPAL
                     GHLIPFSASVFFFIILIFPGTLTSDAVQKENRNK"
     gene            643..>1270
                     /locus_tag="PK683_19150"
     CDS             643..>1270
                     /locus_tag="PK683_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004484433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH10628.1"
                     /translation="MPIQINRKHSLDRLKKTQFDCIFIGGGATGTGAALDATLRGLNT
                     ALLEKRDFSAGTSSKSTKLIHGGVRYLAQFHFKLIREALLERKRLLANAPHLVKPLKF
                     VLPCYKLLERPYYGIGLTFYDILAGDNGLPTHRSISKTETIREFPSINPIGLTGAITY
                     YDAQFNDARLNVLLAQSAAVEGACVTSRTEAVSFIKENGKITGVNVRDI"
BASE COUNT          370 a          284 c          256 g          360 t
ORIGIN      
        1 gtctctttcc attcaggtgg atcttttcaa gacaagtcac agttctctca gattcaatat
       61 gctgtcaggt cttgcccttt ttttcagctc ccttctgcct ctttttcttc ataagtcatt
      121 ttcggaaggg ctggtagatg caggactgaa ggcagcctca ttcacatacg gccctctctt
      181 gggactcttc cttctggcga gaatttcgaa gtcccatatc agttacagtc agaaaaatct
      241 gaatatggct cttttgctct cacttttttc ctgcatagga ttctttcttc ttttcaggcc
      301 ggcactgggg cacctgattc cattcagtgc atcagtcttc tttttcatca tactgatttt
      361 tccgggaact ctgacttctg atgcagtcca gaaggaaaac cggaataaat gaattctaaa
      421 tattttgata tactgattaa aattacagtc tttgacggtg tccagatttt cgccgactag
      481 gtcagcccca gtctttccgt taaaaatcag gaagaaatcc cgattctgat tctttcggag
      541 aaaaagatct gtatttcggc acagaagaaa tagctagaaa aaaaaagaag tatctgagta
      601 attgtccatt aaacaaaaat caaaacctga cggagaggga tgatgcctat tcagatcaac
      661 agaaaacaca gcctcgacag actgaaaaaa actcagtttg actgcatttt tataggggga
      721 ggggctacag gaactggtgc tgcactggat gcgactctca gaggtctgaa tactgctctt
      781 ctggaaaaaa gagatttttc tgcaggcaca tccagcaaat ctacaaaact cattcatgga
      841 ggagtcaggt atctggcaca gtttcatttc aaactgatca gagaagccct tctggaaaga
      901 aaaagacttc ttgcaaatgc ccctcatctg gtaaagcctc tgaaattcgt tcttccctgt
      961 tataaacttc tggaaagacc atattacgga atcggcctca ccttctatga tattcttgcg
     1021 ggagataacg gtcttcctac tcacagaagt atttccaaaa cagaaaccat ccgtgaattc
     1081 ccttcgatca atccaattgg actcacagga gcaatcacct attacgatgc acagttcaat
     1141 gatgcaagac tcaatgtgct gttagcccaa agtgctgcag tggaaggtgc ctgcgtgaca
     1201 tccagaacgg aagcagtcag tttcattaag gaaaacggaa agattacagg agtaaatgta
     1261 agagacatcc
//
LOCUS       DAOQGJ010001505         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87567_length_1268_cov_3.515251, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001505 DAOQGJ010000000
VERSION     DAOQGJ010001505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87567_length_1268_cov_3.515251"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PK683_19155"
     CDS             <1..356
                     /locus_tag="PK683_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10629.1"
                     /translation="EYFTVGEFISGDSSADPKVRLRLTVSSEFHLEDLVLQMEEEGWL
                     SEWDYGTDLKSQYFVFSEEPSKTQIDSIASRFIPVIEEIISSEHFWEAGYLGLIQLFV
                     MVMIGRKYQEAEIEI"
     gene            346..1014
                     /locus_tag="PK683_19160"
     CDS             346..1014
                     /locus_tag="PK683_19160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNH10630.1"
                     /translation="MKYKYIFLDIDDTLYEYRPCHEAGFRALCSFVSETFSVSMERAE
                     QYYQESRKEVGTRLHGQGASHSRLLYSQGICERLNVSPAEFALQIERKYLDKFLVTIQ
                     LRPFVNEFLDTAIRKGCSLGVITDLTAGIQFEKIKRFGIQNKIHTIVTSEEAGAEKPD
                     FRIYQLALEKLKAEKSLSIMIGDSMEKDVLGARRFGISACLLGRQEGENEQTESFQDL
                     LKYL"
     gene            1011..>1268
                     /locus_tag="PK683_19165"
     CDS             1011..>1268
                     /locus_tag="PK683_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10631.1"
                     /translation="MNEKLIDDLLLLSRRTGERFDLVQSGGGNSSVKTSDGKMYVKAS
                     GLRLSDLSREKGLSLIDNEQLKKDFHSTDWTSFDKKGREKKA"
BASE COUNT          391 a          234 c          317 g          326 t
ORIGIN      
        1 tagaatactt tactgtaggc gagtttatca gcggggacag ttcagcagat ccgaaggtca
       61 ggctccgact tactgtcagc tccgagtttc atctggaaga tcttgtcctg cagatggagg
      121 aggaagggtg gctttcggaa tgggattatg gaactgacct gaagagccag tattttgtct
      181 tctccgaaga accttcaaaa actcaaattg attcaatcgc atcaagattc attcctgtaa
      241 ttgaagaaat catcagttcg gagcatttct gggaagcagg atatctgggt ctgattcagc
      301 tttttgtgat ggtaatgatc ggaagaaaat atcaggaagc agaaattgaa atataaatat
      361 atattcttag acatcgatga tacactctat gaatatagac cctgccatga ggccgggttt
      421 agggcactgt gcagttttgt atctgaaaca ttcagtgttt ccatggagag ggcagagcag
      481 tattatcagg aatccagaaa agaggtggga accagacttc acgggcaggg agcatcccac
      541 agcagactcc tttactcgca ggggatctgt gaaagactga acgtcagtcc ggctgaattt
      601 gcccttcaga tagaaagaaa atacttggac aagtttcttg tcaccataca gctccgtccc
      661 tttgtaaatg aatttttgga tactgccatc cgaaaagggt gcagtcttgg ggtgattact
      721 gatcttaccg caggaattca gttcgaaaaa ataaaaagat tcgggattca gaataagatt
      781 catacgattg tcacaagcga agaggctggg gcagaaaaac ccgatttcag gatataccaa
      841 ttagcacttg aaaagctgaa ggcggaaaag agtttatcca taatgatcgg ggacagtatg
      901 gaaaaagatg tacttggtgc gagacgtttc ggaatttctg cctgcctgct cggaagacag
      961 gaaggagaaa atgagcagac tgaaagtttt caggatctct tgaaatatct atgaatgaaa
     1021 aactgattga tgaccttctt ctgctttcca gaaggacagg ggagcggttt gatcttgttc
     1081 agtccggtgg aggcaattcc tctgtcaaga catcggatgg aaaaatgtat gtgaaggcat
     1141 cgggactcag gctttctgat ctttccagag aaaaaggcct ctctctcatt gacaatgaac
     1201 agctgaagaa agacttccat tcaaccgact ggacttcgtt tgataagaaa ggaagagaaa
     1261 aaaaagca
//
LOCUS       DAOQGJ010001506         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87624_length_1268_cov_2.178895, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001506 DAOQGJ010000000
VERSION     DAOQGJ010001506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87624_length_1268_cov_2.178895"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..692)
                     /locus_tag="PK683_19170"
     CDS             complement(24..692)
                     /locus_tag="PK683_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10632.1"
                     /translation="MYSSFFLIPVFYFAKKREKGTEETFLLWVTVVFMLFVGFTSPNN
                     GITINGRYQLLVILPLSRMMQLWSDAQSEQKKSKLVIALVSFSVLFSAVAVSVLKYAY
                     GRQREARVILQNIKTDSYVVPNSFLCNMIGLEHLAKPVFCIKNETSIDLIDQIIKGNK
                     SLKIVTLFNFDEKKNKPFIDSNSALNPSHQESLIKRMETNCKKADKIEDMGFTLQRFV
                     CENE"
BASE COUNT          470 a          234 c          209 g          355 t
ORIGIN      
        1 gaatcgattt ttatcggaca gcactactca ttctcacata caaaccgctg cagggtaaac
       61 cccatatctt caattttatc tgctttttta cagttcgttt ccattctttt aataagagac
      121 tcctgatgcg aagggttcaa ggcgctgtta gaatcaatga aaggtttatt ctttttttca
      181 tcaaaattga atagcgtaac aattttcagg cttttgtttc ccttaattat ctgatctatc
      241 aaatctatac tagtctcgtt ttttatacag aaaactggct ttgcaagatg ttctaatcct
      301 atcatattgc ataaaaagct gtttggcaca acatacgaat cagtttttat attctgaaga
      361 attactcttg cttccctttg ccggccatag gcatatttta aaactgaaac tgcaaccgca
      421 gaaaacaaaa cagaaaagga aactaaagca ataacaagtt tacttttttt ctgttctgat
      481 tgagcatcag accacagttg catcattctg gacaagggaa gaataacaag caactggtat
      541 cttccattta tagttattcc attattaggg gatgtaaaac cgacaaagag cataaagacc
      601 actgtcaccc ataacagaaa agtctcttct gtaccctttt ctcttttttt tgcaaaataa
      661 aagacaggaa tcagaaaaaa actggaatac atcagcatac caattctagg aaccccgtca
      721 aaataaaaaa agcttatgga aagaagattg ataattcgat ccagaacagt aatattaccg
      781 gcaccgaaat taaatatgta ccttaatcct agaatgtgac cgtagttggt aaaattccat
      841 acaaacagga aaaataatgg aatcaggcct ataactaaaa aaagcagaga tttctttttg
      901 agaaggtcta tgaatacctc ccttcttgca aaaaaaaggg caataaagag agataatatg
      961 aacagtattg attcaagcct aaaccataca gaaagagaga ccgcaaaaat cgaatacaga
     1021 agaaactcag ttcgcttatc atcaagactt gactgaagat attccagcca atagtaaaat
     1081 cctatagtat tcaataaaaa aaacagaccg ttttcactga attcaaaaca taacggaaag
     1141 attattgaac cgaaaaacac tgtaagtgaa gtttgccagg atacttttcc tttgctgaca
     1201 agaaatgcca ttgatacagc gaagggtaat gaggcaagaa agggaaaaat ttcgaaaggg
     1261 aaagacag
//
LOCUS       DAOQGJ010001507         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87651_length_1268_cov_1.761748, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001507 DAOQGJ010000000
VERSION     DAOQGJ010001507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87651_length_1268_cov_1.761748"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PK683_19175"
     CDS             complement(<1..1251)
                     /locus_tag="PK683_19175"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNH10633.1"
                     /translation="MSATLDASSLQKLIPGSEILSAGGKMFPVSVRYIKAEKSLSRDS
                     VLTSVRSAVGLPGDILFFLPGMREIRYAESLVREVMTDTDIEIRILHSEIPASEQEEI
                     LRGGGKKKRLILSTNIAETSLTVPGVMTVIDSGLVKQMRYSPSNGMEELVTERISLSS
                     AEQRKGRAGRLSEGTVFRLYSVQEEKEFSEFPEPEILRTDLSAPILESLNYGVGFREL
                     SFLEKIPESKVTAAETVLQEIGVLDSARNLTELGRKISRFGIHPRLSAMILRSSEIGL
                     EVQACRLSAVLSEQDVQENEFRFRSDLNLRLERIFRGSSDYRMRKIHSLSEDLLKRTE
                     NTKKYDVFHPEKTGLLSAFAYPERIGRKKGGGKFKLASGKLAVCAEDDPMAENEFICA
                     AEITGDGTDNRIRRSAAVQLTDLLK"
BASE COUNT          307 a          319 c          249 g          393 t
ORIGIN      
        1 cttcagaaga tcagtcagct gaactgctgc agatcttctg attctgttgt ctgttccgtc
       61 cccggtaatt tcagctgcac agatgaattc attttctgcc atcggatcat cctctgcaca
      121 taccgcaagt ttccctgaag ccagcttgaa ctttcctccg cctttttttc tgcctattct
      181 ttccggatag gcaaaggcag aaagaaggcc ggtcttctcc gggtgaaata catcatattt
      241 ttttgtattc tctgttcttt tcagaagatc ctctgaaaga gaatgtattt ttctcatcct
      301 gtaatcggat gatcctctga agattctttc cagtctcaga ttcagatcag acctgaatct
      361 gaattcattt tcctgaacat cctgctcgga aagaactgca ctcagtctgc aggcctgaac
      421 ctccagaccg atttcagagg aacgaagtat cattgccgaa agcctgggat gaattccgaa
      481 tctggaaatt tttcttccaa gttcagtcag attcctggct gaatcgagca cgcctatttc
      541 ctgaagaact gtttcagccg ctgtgacttt tgattcagga attttttcga gaaaggaaag
      601 ttcacggaac ccgacaccat agttgagact ttccagaatc ggtgcagaca ggtctgttct
      661 taatatctct ggttcaggaa actcggaaaa ctctttttct tcctgaacgg aatacagtct
      721 gaatacagtg ccttctgaaa gacgtccagc ccttcctttc ctctgctctg ctgaactcag
      781 agaaattctt tctgtcacaa gttcttccat tccgtttgaa ggagaatatc tcatctgttt
      841 tacaagcccg gaatcaatga ctgtcatcac tccgggaacc gtaagactgg tttctgcaat
      901 gtttgtactc agtataagcc ttttcttttt tcctcctcct ctcaggattt cctcctgttc
      961 agaggcagga atttcactgt gcagtatcct gatttcgata tcagtgtctg tcatcacctc
     1021 tctgaccagc gactctgcat atctgatctc ccgcatccca ggcagaaaaa acagaatgtc
     1081 tccaggcaga ccgacggcag atctgactga cgtcagtacg gaatcccttg aaagtgattt
     1141 ttctgccttt atgtatctta ctgatacagg gaacattttt cctcccgcac tcaggatttc
     1201 tgaaccagga atcagtttct gaaggctcga tgcatccagt gtggcggaca ttatcataag
     1261 cctgcatt
//
LOCUS       DAOQGJ010001508         1267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87704_length_1267_cov_2.985974, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001508 DAOQGJ010000000
VERSION     DAOQGJ010001508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87704_length_1267_cov_2.985974"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="PK683_19180"
     CDS             <1..1123
                     /locus_tag="PK683_19180"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH10634.1"
                     /translation="IQKGADIREKDDFGRTVLHSASQSDSLDIIRHLIEELQFNVNEK
                     DHNDETALHSACVNGRLETVKYLVEKGADVNALVRARTDGNSPLDIACFKGFHEIAEY
                     LINKGADIFRKDGNGWTFLFTACEGGNLDIVKLLIDKGLNINAKTEDGRTLLFEACQH
                     GHLHLLEYLLSLGLDINAKTKEDLTLLHYGCQGGNIELIKFLYNKGLDINAVTKDGQT
                     PLYIASGCGNLQLVKFLTENGADIQKADSGGKTSLSNAGSVNYLDVVRFLHEKGALLN
                     DELLISCADRGYDELAEYVLKNGMNANVKNTEGWTALHFASENGYSSKMIQLLIDYHT
                     DINSKVQSGEKKGYTALKLAKENEHKEIIKILKKCGAEE"
BASE COUNT          424 a          240 c          303 g          300 t
ORIGIN      
        1 gatccaaaag ggagctgata tccgcgaaaa ggatgatttc ggaaggaccg ttctgcattc
       61 tgcaagccag tccgacagtc tcgatataat caggcatctg attgaagaat tacaatttaa
      121 tgtaaatgag aaagatcata atgatgaaac ggctctccac tctgcctgtg tcaacggccg
      181 gttagaaaca gtaaaatacc ttgtggaaaa aggagctgac gtcaatgcgc tggtgagagc
      241 cagaacagac ggaaattctc ctctggacat cgcctgcttt aaagggttcc atgagattgc
      301 agaatacttg ataaataagg gagcagacat attcagaaag gatggaaacg gatggacctt
      361 cctttttact gcctgtgaag gcggaaatct cgatattgta aaactgctga tagacaaagg
      421 gttgaatata aatgcaaaga cagaggacgg gagaaccctt ttattcgagg cctgtcagca
      481 cggtcatctt catcttctgg aatatctgct gagcctggga ctggacataa atgcaaagac
      541 caaggaagat ctcactctgc tgcattacgg atgccagggc ggcaatatcg aattaataaa
      601 gtttctatat aataaaggat tagatataaa tgcagttaca aaggacggac agacccctct
      661 gtacattgcc tccggatgcg gcaatcttca actggtcaaa tttctgacag aaaacggtgc
      721 tgatattcaa aaagcggaca gcggagggaa gacatctttg tccaatgcag ggagcgtcaa
      781 ttatctggat gttgtaaggt tcctgcatga aaaaggtgca cttctgaatg atgaacttct
      841 gatttcctgc gcagacagag gctatgacga acttgccgag tatgtgctga aaaacggaat
      901 gaatgcaaat gtcaaaaaca cggaaggatg gacggcactg cactttgcca gtgaaaacgg
      961 atattccagt aaaatgattc agctgctcat tgattatcac acagacatca acagcaaagt
     1021 acagtcagga gaaaaaaagg gatatactgc actgaagctg gcaaaagaaa atgagcataa
     1081 ggaaataatc aagattctga agaaatgcgg tgcagaagag tgaatcgaat gcagtgttta
     1141 tgagatatat tcagtatctc aaaagatccg tatgaatgaa atcctctcct ttgaaaagca
     1201 gaggttctct tttgcccttg gataacgcat aggcaaaaca gtcaccaaaa ttcagagacg
     1261 ctttgtg
//
LOCUS       DAOQGJ010001509         1267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87754_length_1267_cov_2.117987, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001509 DAOQGJ010000000
VERSION     DAOQGJ010001509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87754_length_1267_cov_2.117987"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..>1267
                     /locus_tag="PK683_19185"
     CDS             93..>1267
                     /locus_tag="PK683_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNH10635.1"
                     /translation="MNSTSGSGKSSLAFDTIFAEGQRRYVESLSSYARQFLGQMDKPD
                     VDLIEGLSPAISIEQKTTHRNPRSTVGTVTEIYDYLRLLYARTGKPHCHICGTPISSM
                     SVDQIVDRIMLFQEGTKLQILSPVVRAQKGEHKDTIEKIRKEGFTRIRLNGEALSLDE
                     NIELKKNIKSSIDIVVDRLVMKPEIRTRLADSVETALKHSSGNLTVNDSETDHTFSRT
                     LSCPNCNNVSIPELEPRIFSFNSPEGACPSCDGIGSLLEFDENLIVNDPESSLIDGCL
                     EVWGKTGSYWYLATVHALAKTLKFDYNTPWKSLPANIKKIILYGDKKIKIEYDFRKED
                     SHYEFAREFEGVIPNLKRRYRETKSDSMRQWMESYMTNHACPACRGRRLRPESLAVKV
                     "
BASE COUNT          382 a          287 c          302 g          296 t
ORIGIN      
        1 ccgggtggcc gaagcggtcc ggcggattca gacgggctac gatcagccgc tgaaggtgcg
       61 cgacttggcg cggcaagccg gactttcggt ctatcaattc gaccagcgga tccggaaaat
      121 cttcgcttgc attcgacact atctttgctg aaggtcagag gaggtatgtg gagagtctat
      181 ctagttatgc gaggcagttc cttggacaga tggacaagcc tgacgtggat cttatagaag
      241 gtttaagtcc agccatttcc attgaacaga agacgactca cagaaatccc agatccacag
      301 taggcactgt cactgaaata tatgactatc tccggcttct ctatgcaaga acgggcaaac
      361 ctcactgtca tatctgcgga actccgattt cctccatgtc agtagaccag atcgtggaca
      421 ggatcatgct tttccaggaa ggaaccaaac tccagattct ctctccagtt gtgcgtgcac
      481 agaaaggaga gcacaaggat acaattgaaa aaatccgcaa ggaaggtttt acgaggatca
      541 ggctgaacgg agaagccttg agtcttgacg aaaacattga actgaaaaaa aacatcaagt
      601 cgagcataga catcgtagtg gacagactcg tgatgaaacc tgagatcaga acaagacttg
      661 cagattccgt ggagacagca ctgaaacatt cctccggaaa tcttactgta aatgattccg
      721 aaacagatca tacattttcc agaacactct cctgtccgaa ctgcaacaat gtatccattc
      781 ctgaactgga accgcgaata ttttccttca attcccctga aggagcctgt ccttcctgcg
      841 acggcatagg aagcttactg gaatttgacg aaaatctgat tgtgaatgat ccggaaagtt
      901 ctctcattga cggatgtctt gaagtctggg gaaagacagg aagctactgg tatcttgcca
      961 cagtccacgc acttgccaag acactgaaat tcgactacaa caccccctgg aagtctctgc
     1021 ctgcaaatat taaaaaaatt atattatatg gtgataagaa aatcaagatt gaatatgatt
     1081 ttcggaagga agactctcat tatgaatttg cccgtgaatt cgaaggagtg atcccgaatc
     1141 ttaagagaag atacagggag acaaaatcag attcgatgag gcagtggatg gaatcctata
     1201 tgacgaatca tgcctgccct gcctgcaggg gaagaagact ccgtcccgaa agtcttgcag
     1261 tgaaagt
//
LOCUS       DAOQGJ010001510         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87822_length_1266_cov_3.885219, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001510 DAOQGJ010000000
VERSION     DAOQGJ010001510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87822_length_1266_cov_3.885219"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PK683_19190"
     CDS             <1..619
                     /locus_tag="PK683_19190"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNH10636.1"
                     /translation="NTANYNPALFRFNSSLTYLGCTALPVSGNNTFSVSLSLDTAQSN
                     VYISGYANGASFESQTVSGSWDAFLTKLTTASGTKLWTKLIGASGSVTTGTSTGFDSN
                     GYVYLAGMTSASLGTQTIAGSQDMFIAKYDSAGTLQWVKLQGIQGGSSHGGCQIPANP
                     GGCSSAVSFDSNNTLYTSGATNGTISGVSNPASPNSSLFLVRNVK"
     gene            750..1229
                     /locus_tag="PK683_19195"
     CDS             750..1229
                     /locus_tag="PK683_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNH10637.1"
                     /translation="MKINSLKRNIRRGLTLVELAVVVLIMGALITLVALNIKPNELKD
                     QTAGLKLRKDASELMMFLEQYSERYGKYPSEEQGIMALVEKPSTGDVPDNYKPIVSNK
                     KAILDPWSTPYHLKTDANGDLQIISLGKDKKEGGEGKNRDFNILKEEEYPEEFLQKK"
BASE COUNT          399 a          285 c          288 g          294 t
ORIGIN      
        1 aaatacagca aactacaatc ctgctctttt ccgttttaat tccagcctaa cctatctggg
       61 atgcactgca ctccccgtct ccggcaacaa tacattttct gtaagtcttt cactggatac
      121 cgcacagtcc aatgtctata tctcaggata tgcaaacggg gccagtttcg aaagtcagac
      181 agtgtccgga tcctgggatg catttctaac caaactcaca accgcatcag gcacaaaact
      241 ctggactaag ctgatcggtg catcaggctc agtgacaaca ggaacaagta caggatttga
      301 ttcaaatgga tatgtgtatt tagccggtat gacaagtgcc agccttggaa ctcagaccat
      361 cgccggatca caggatatgt tcattgcaaa atatgattcc gccggaactc tgcagtgggt
      421 aaaacttcag ggaatccagg gcgggagttc ccacggagga tgtcagattc cggccaatcc
      481 tggcgggtgc tcaagtgccg tttcctttga ttccaacaac acactgtata catccggggc
      541 gacaaacggc acaatcagcg gggtttcaaa tccggcctca ccgaattcat cccttttcct
      601 tgtcagaaac gtgaagtgag aagagtaaaa tttcccttga ctaaagaaat tctgcctgca
      661 gaatataatt tacggatttc agtgaaatca atgaaatctg tatccaaaat caattgatct
      721 acagtcaaaa agaacagaag gagactgtca tgaaaattaa cagcctgaaa agaaatatca
      781 gaagaggact gacgctcgta gaactggcag tggtcgttct cataatggga gccctcatca
      841 cactggttgc attgaacatc aagccgaatg aactgaagga tcagacggca ggactgaagc
      901 tgagaaagga cgcgagtgaa ctgatgatgt tcctcgagca gtactcggaa aggtacggaa
      961 aatatccgag tgaagaacag ggaattatgg ctctcgtgga aaagccttcc actggagatg
     1021 ttcccgacaa ttacaagccg atcgtttcca ataaaaaagc gatcctggat ccttggagca
     1081 ctccgtacca tctgaaaaca gatgcgaacg gtgatcttca gatcatttcc ttaggaaaag
     1141 acaagaaaga aggcggcgaa ggaaaaaatc gggacttcaa tatcttgaaa gaagaagaat
     1201 atccggaaga atttcttcag aaaaaataac tttctgcaga ctgtgtgatt cagtctgcac
     1261 agtctg
//
LOCUS       DAOQGJ010001511         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87831_length_1266_cov_3.614368, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001511 DAOQGJ010000000
VERSION     DAOQGJ010001511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87831_length_1266_cov_3.614368"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PK683_19200"
     CDS             <1..554
                     /locus_tag="PK683_19200"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10638.1"
                     /translation="YSHNRMDGQIVHVIPLRIDLKKFTKTKSQKKIWQKNADTEFKFQ
                     EIQIDETKIELFRIHAERFESNRPTSVFDFLSKNTPFRPCYSEECCVYIDGKLGAVSF
                     LDIGSSSTSSVYGMFDPDLSKRSLGIYTMLLEIEYSIQMNKDYYYPGYAYVETSFYDY
                     KKNFNGLEYLDWNKGWKPFIKTH"
     gene            527..1204
                     /locus_tag="PK683_19205"
     CDS             527..1204
                     /locus_tag="PK683_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNH10639.1"
                     /translation="METVHKNSLTLQLEEAVENHTVLKSDWLLEKSENLSLKSLHLWL
                     SQEYFVSVDFVNWFLITASLTDNQEAKTILVENIWEELGEGNTKNSHVSILIQFLQET
                     GFNFENHILFQETKIYLDTMRKIIARGFLFGLGALGPANEYLLKLEYGKMYQAYRKLA
                     SEKTLPRAHFFEVNLHADESHSAKMFRLIENSVKTDADFSQVMEGNLSALEARKLFYE
                     GLKRKEG"
BASE COUNT          423 a          243 c          263 g          337 t
ORIGIN      
        1 gatacagcca caacagaatg gacggtcaga tagtccatgt aattccgctc cggattgatc
       61 tgaaaaaatt cacaaaaaca aaaagccaga aaaaaatctg gcagaaaaat gccgatacgg
      121 aattcaaatt tcaggaaatt cagattgacg agaccaagat agagcttttc agaattcatg
      181 cagaaagatt tgaatcgaac agacctactt ccgtttttga tttcctgtcc aaaaatacac
      241 ctttccgtcc ctgctattcc gaggagtgct gcgtgtacat tgacggaaaa ctaggggcag
      301 tcagctttct cgacattgga agttcttcta catccagtgt atacggaatg tttgatcctg
      361 atctgtcaaa gagaagtctc ggaatttata cgatgcttct ggaaatcgaa tactctattc
      421 agatgaataa agattattac tatccaggat atgcatatgt tgaaacctca ttctatgatt
      481 acaaaaaaaa ttttaacggc ctggagtatt tggattggaa caaaggatgg aaaccgttca
      541 taaaaactca ctgactcttc agcttgaaga ggcagttgag aatcatactg tgctgaagtc
      601 agactggctt ctggaaaaaa gcgagaacct ttccttgaaa agtcttcatc tatggctttc
      661 tcaggaatat tttgtctcag tggattttgt aaactggttt ctgatcaccg cgtcattgac
      721 agacaatcag gaagccaaaa ccatcctcgt ggaaaatata tgggaggagc tgggagaagg
      781 aaacacgaaa aacagccatg tttccatcct gatacagttt cttcaggaga ctggattcaa
      841 ttttgaaaac catatcctgt ttcaagaaac aaaaatatat ctggatacaa tgagaaagat
      901 tatcgcaaga ggattccttt tcggtcttgg agctctcgga ccggcaaatg aataccttct
      961 taagctggaa tatggaaaga tgtaccaggc atacagaaag cttgcttcag aaaaaactct
     1021 tccgcgagca cattttttcg aagtgaatct gcatgcagat gaatctcaca gtgcaaaaat
     1081 gtttcgactg atagaaaact cagtcaaaac agatgctgac ttttctcagg taatggaagg
     1141 aaatctttcc gcactggaag caagaaaatt attttacgaa ggattgaaaa gaaaagaagg
     1201 ctgaaacctt ccggatcagc ataagagctt ttctttcagt caagagactg tgtatacgcg
     1261 gctccg
//
LOCUS       DAOQGJ010001512         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87832_length_1266_cov_3.596201, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001512 DAOQGJ010000000
VERSION     DAOQGJ010001512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87832_length_1266_cov_3.596201"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PK683_19210"
     CDS             <1..763
                     /locus_tag="PK683_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNH10640.1"
                     /translation="LFDEYPTELPSEEPGEIETQIGKNVASLIQDEATIQMGIGGIPN
                     ATLKCLFDKKDLGIHTEMFSDGILPLLEEGVITNEKKEFHRGKIISGFVMGSQDLYNY
                     IDNNPLFEFHPSHYTNDPFRIARNHRMTAINSAIEVDLTGQVCADSMGEMIYSGIGGQ
                     VDFIRGAARAKEGRPIIALPSTAKGGKVSRISLFLNKGSGVVTSRGDVHYIVTEYGIA
                     NLFGKNLRQRAEALISIAHPDFRAELRKDSKWALK"
     gene            1079..>1266
                     /locus_tag="PK683_19215"
     CDS             1079..>1266
                     /locus_tag="PK683_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10641.1"
                     /translation="MESVIDIRKEENVIQQLESVLNEILYAEQNNAARAAFLEATRGL
                     KPESCNVRTGWSAGQWIA"
BASE COUNT          371 a          246 c          314 g          335 t
ORIGIN      
        1 gctgtttgac gagtatccta ctgaacttcc ttccgaggag ccgggagaaa tagaaactca
       61 gatcggcaaa aatgtcgctt ctctgattca ggacgaggct acaattcaga tggggatagg
      121 cggtatccca aatgcaacat taaaatgtct tttcgacaag aaggatcttg gcattcacac
      181 tgagatgttc agtgacggaa tccttcctct tcttgaagaa ggcgtgatta caaatgagaa
      241 aaaggaattt cacagaggaa agattatttc gggattcgtt atgggatctc aggatcttta
      301 taattatatt gataataacc ccttatttga attccatcca agccattaca caaatgatcc
      361 tttccgcatt gcaagaaacc acagaatgac tgcaatcaac tcagcgattg aagtggatct
      421 gactggtcag gtctgcgcgg acagtatggg ggaaatgatc tacagcggga ttggaggaca
      481 ggtggacttt atccgaggag cagcaagagc gaaagaagga agacctatca ttgcacttcc
      541 ttccactgca aagggcggaa aggtttcccg gatctctctt ttcctgaaca agggttccgg
      601 agttgttaca agccgcgggg atgttcatta tatcgtgact gaatacggaa ttgcaaacct
      661 gtttggaaag aatctcagac agagagcaga ggcactgatc tccattgcac atcctgattt
      721 cagagcagaa ctcagaaaag attccaagtg ggctttgaaa tagaataata tttagggatt
      781 gtgtaaagac tgaattgcac caccgatgaa cacggacagg cacggatcac tgttgataat
      841 taattcaatg cggtctgcct gttcttcaga gggaatttca gcttttagac agttcctttt
      901 aaagctcctt gatgcatttt actgaatgaa cagttttttt ggggaggctg tttcttcagc
      961 atagccttcc cgtcagagat tcattcttga tatgctgata accgcttgac ctgttttcag
     1021 tcgacggaca gaattggatt tggcgacggg actttatgac aggagggctt aaattcgaat
     1081 ggaatcggta atagacatca gaaaagaaga aaatgtgatt cagcagttgg agtcagtcct
     1141 gaatgagatc ttgtatgcgg agcagaacaa cgctgccaga gctgcttttc ttgaggcaac
     1201 cagaggtttg aaacccgaaa gctgtaatgt aaggacaggc tggtcggcag gtcaatggat
     1261 cgcatt
//
LOCUS       DAOQGJ010001513         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87853_length_1266_cov_3.067713, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001513 DAOQGJ010000000
VERSION     DAOQGJ010001513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87853_length_1266_cov_3.067713"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK683_19220"
     CDS             <1..277
                     /locus_tag="PK683_19220"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNH10642.1"
                     /translation="IQGIGPGFVPDVLNTKVIDEVVKVTNQDAGETARRLAKEEGILA
                     GISSGAAVWAALEVASRDSSAGKTIVVVAPDTGERYLSTWLFEEFRN"
     gene            328..>1266
                     /locus_tag="PK683_19225"
     CDS             328..>1266
                     /locus_tag="PK683_19225"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10643.1"
                     /translation="MVNNKEQKFLNEDDLKFSHFFPHHHRKDGKTVSAVLDRTVSLLT
                     DQKSEVHEFIYRQGKLLPELTSLRNIVDLFRSIVFPGYFSSQFDRPISLNYFTGRNLE
                     KLYELLKEQIHNGFCFAEEEDTEELRNTAADLALKFIERIPEIRRILATDVKAMYNAD
                     PSAKNFEEIIFCFPTVRTMTNYRIAHELLKLGVPLIPRIISELAHSETGIDIHPGAEI
                     GEYFCIDHGTGVVIGETCIIGNNVRLYQGVTLGAKRFNLDENGNPLKDDPRHPIIEDG
                     VVIYSNATILGRITVGANSVIGGNVWITDNVPPNSKI"
BASE COUNT          378 a          254 c          300 g          334 t
ORIGIN      
        1 gatccagggt attggaccag gttttgttcc tgatgttttg aatacaaaag tcattgatga
       61 ggtcgtgaag gtcacaaatc aggatgcagg cgaaactgcc agaagacttg ccaaagagga
      121 aggaatcctt gcaggcatct cctccggtgc tgccgtctgg gctgcgcttg aagtggcatc
      181 gagagattcc agtgcaggaa aaacgattgt agtcgttgcc cctgacaccg gggagagata
      241 tttaagcact tggctttttg aagagttcag gaactgattt ttcagtgctt ggagagagtt
      301 ttacagagta taccctggag gttccatatg gtaaacaata aggagcaaaa atttctgaat
      361 gaggatgatc ttaagttttc acatttcttt cctcaccatc acagaaaaga cggcaaaact
      421 gtttctgctg tattagacag aactgtaagt ctattgactg atcagaaatc agaagttcat
      481 gaatttatat acagacaggg aaaacttctg cctgaactga ccagcctcag gaatattgtg
      541 gatctgttcc gttccattgt atttccgggt tatttcagtt ctcagtttga cagaccgatt
      601 tcactgaact attttacagg aagaaatctg gaaaaactgt atgaacttct gaaagagcag
      661 atccataacg gattctgctt tgcggaagaa gaagacacag aggagctcag gaatacggca
      721 gccgatcttg cactgaagtt cattgagaga attcctgaga tcagaagaat tcttgcaacg
      781 gatgtgaagg caatgtacaa tgccgatcct tctgccaaga attttgaaga gattatcttc
      841 tgttttccta cagtccgaac aatgaccaat tacagaattg ctcatgaact tctgaaactt
      901 ggcgttcctc tgatccccag aatcatatct gaacttgccc attctgaaac agggatagac
      961 attcatcccg gtgcagaaat cggagaatac ttctgcattg atcacgggac aggagtcgtc
     1021 attggagaaa catgcattat agggaacaat gtccggcttt accagggagt gactctgggc
     1081 gcaaaaagat tcaatctgga tgagaacgga aatcctctga aagacgatcc gaggcatccg
     1141 attattgaag acggcgttgt catttactca aatgccacaa tactcggacg gatcacagtc
     1201 ggtgcaaatt cagtaatagg cgggaatgtc tggattacgg acaatgttcc gcccaattca
     1261 aagatc
//
LOCUS       DAOQGJ010001514         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87892_length_1266_cov_2.291495, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001514 DAOQGJ010000000
VERSION     DAOQGJ010001514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87892_length_1266_cov_2.291495"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1131
                     /locus_tag="PK683_19230"
     CDS             202..1131
                     /locus_tag="PK683_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10644.1"
                     /translation="MLRTIFFILLFTVMIGAQDFPAYILERNLSGKSMQIFYSEKKGI
                     SNLLYGRGFFEFEKLPVFVGAGFGQQKRTAVAEDVLQLTEKKEKLGRAVSGFSVQSAS
                     VYAGKEFLNSERSVLGIQFRLFAEKKIQFEQTWGTGFKRDAVLLQSGKDLILGLNFYR
                     TEDSSRNFTEKGGSVSVTLNFDFFSASAGALFGSSDWKETVFLSASVHQNGKETALLS
                     EQNVFEPISSDRPENEERIPEKKPAPPPKDNEKKPVILTLQELLARKIPLGEAARIAE
                     ASKDPKKLKALLEILPKDTRGKVHFLLKEKKSK"
BASE COUNT          407 a          243 c          255 g          361 t
ORIGIN      
        1 aaacatatat tagtattaca ctaatttttg tatagcattt ttttgttccg ttcggttttt
       61 ttccttatct atcagtatac ggttcaggga gacaaaatga actgactgat ttgcattttt
      121 cgattttttt tcagaaaaaa gcgaattttc tgattttttt actcaaatct gagtattttt
      181 caatccggta cagacagaac tatgctcaga acgatttttt ttatccttct tttcaccgta
      241 atgatcggtg cacaggattt tcctgcctat attctggaaa gaaatctgtc aggaaaatca
      301 atgcagattt tttattcaga aaaaaaagga atctcaaatc tcctttacgg aaggggattt
      361 tttgaattcg aaaaactccc tgtttttgtc ggagcaggat ttggtcagca gaaaagaact
      421 gcagtggccg aagatgtgct tcagctgact gaaaaaaaag aaaaactcgg aagggccgtt
      481 tccggttttt ccgtccaatc tgcttctgtc tatgcaggaa aggaatttct gaacagtgaa
      541 cggtctgttc tgggcattca gttcagactt tttgccgaaa agaaaattca attcgaacag
      601 acctggggaa ccggattcaa aagagatgcg gttcttctgc agagcgggaa agacctgatt
      661 ctcgggctga atttttaccg aacggaagac agcagcagaa attttactga aaaaggcggt
      721 tctgtatcag tcactctgaa tttcgatttc ttttccgcat cggccggtgc attgttcggc
      781 agttccgact ggaaggaaac agtttttctt tcagcatcag ttcatcagaa cgggaaagag
      841 acagcactcc tttccgaaca aaatgtattt gaaccgattt catcggacag gccggaaaac
      901 gaagaaagaa ttcctgaaaa aaagcctgct ccccctccga aggacaatga aaaaaaacct
      961 gtcattctca cactgcagga acttctggcc aggaaaattc ctctcgggga agcagccaga
     1021 atagcagagg catcaaagga cccgaaaaaa ctgaaagctc ttcttgaaat tctcccaaag
     1081 gatacaagag ggaaggttca ttttcttcta aaagaaaaaa aatcaaaatg aaatacttaa
     1141 tcattataat tatatcattt acttcctcag caggatcaga aattaaaatg actgcaggag
     1201 gcacgtatga agttcttcag acaagatatg ctgatgcgga aacaaatcag aaaacaaata
     1261 tattca
//
LOCUS       DAOQGJ010001515         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87972_length_1265_cov_2.934711, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001515 DAOQGJ010000000
VERSION     DAOQGJ010001515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87972_length_1265_cov_2.934711"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1165
                     /locus_tag="PK683_19235"
     CDS             161..1165
                     /locus_tag="PK683_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNH10645.1"
                     /translation="MTTQLTKKDFVSNQEIRWCPGCGDYGILAAVQKTLPELGVPLEK
                     FAFISGIGCSSRFPYYINTYGFHTIHGRAPAVASGLKIANPDLSVWVITGDGDGLSIG
                     GNHLIHAIRRNLDINIIFFNNRIYGLTKGQFSPTSALGSITKSSPYGTIDPPFRPLSV
                     VIGAEATFAARTYDKSQKHMSETIEAAFRHKGTSFIEVLQNCAIFNDGVHDDVYGKDV
                     RDDTTVLLEHGKPLIFGANQDKGIVMEGSAARVVSLSEVPAEKIHVHDETVPSPDYAF
                     LLSRMENPEFPVPMGVLRNVKAPVYEDMVQAQIQTAIDKKGEGSLQDLICTEDTWEIK
                     "
BASE COUNT          377 a          245 c          284 g          359 t
ORIGIN      
        1 gataaaatca ttgttcctga actgaatgcg ggtcagctcg catttctact tcagggaacg
       61 tacggagtaa aagtccataa actgactaag attcagggaa agccctttac tgtccaggaa
      121 attgaagcaa aaattgagga actcaaataa ggagaaattt atgacaacac aacttacaaa
      181 aaaagatttt gtatccaatc aggaaatccg ctggtgtccc ggatgcgggg attacggaat
      241 tctggctgct gttcagaaaa ctcttcctga attaggtgtt ccattggaaa aatttgcatt
      301 catatccgga attggatgct cctccagatt tccttattat ataaatacct atggttttca
      361 tacgatacac ggcagggctc ctgctgtggc ttcaggtctg aaaattgcaa atcctgactt
      421 aagtgtctgg gtcattaccg gtgacgggga tgggctttct atcggaggaa atcatctgat
      481 tcatgcgatc cggagaaatc tggatataaa tataatattc tttaacaaca gaatatacgg
      541 actgacaaag gggcagtttt ctcctacttc cgctctcggt tcaattacca agtccagtcc
      601 ttacggaaca atagatcctc ctttcagacc tctctctgtt gtcatcggtg ctgaagcaac
      661 ttttgcagcc agaacctatg ataagtccca gaagcatatg tctgaaacaa ttgaagctgc
      721 tttcagacat aagggaactt cctttatcga ggtgcttcag aactgtgcga tttttaatga
      781 cggtgttcat gatgatgtat acgggaaaga cgtgagagat gatactactg ttttacttga
      841 acatggcaag cctcttatat tcggagccaa tcaagataag ggaattgtga tggaaggatc
      901 ggctgcaagg gttgtaagtc tttctgaggt acctgctgaa aaaatccatg ttcatgatga
      961 aacagttcct tcacctgatt atgcctttct gctttccagg atggaaaacc cggaattccc
     1021 tgttccgatg ggagtcctca gaaacgtaaa agcgcccgtc tatgaagata tggtacaggc
     1081 acagattcag actgcgattg ataagaaggg agaaggcagt cttcaggatc ttatctgcac
     1141 tgaagatacc tgggagataa aatagaatat ttcttaatgt aagtattctg tttatatttg
     1201 taagcagaaa ttgtggtgaa aaacagatga gattgcgtaa gagctgcatt tcaccatgct
     1261 tcgac
//
LOCUS       DAOQGJ010001516         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_87979_length_1265_cov_2.808264, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001516 DAOQGJ010000000
VERSION     DAOQGJ010001516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87979_length_1265_cov_2.808264"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK683_19240"
     CDS             complement(<1..345)
                     /locus_tag="PK683_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH10646.1"
                     /translation="MNFEINEEHRTFLDTFRNFCRKEIEPYTAESEKEGMIPRFHYKK
                     LYEIGYTGLMHEEKYGGSDSGYELSVLAQEILSEYCGSTFFSSGASVGLFGLPLREHG
                     TEEQKEKYLPNII"
     gene            complement(353..>1265)
                     /locus_tag="PK683_19245"
     CDS             complement(353..>1265)
                     /locus_tag="PK683_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH10647.1"
                     /translation="GGMDGGLTLAWGAHSIIGCMPIVLCGTDAQKKKYLPRLASGEWT
                     AALGLTEPSSGSDAAGSMETRAVKKGDKYIINGSKMFITNGPIGDVFIVMAVTDKAKG
                     AMGVSVFIVEKNFKGFKTGKKLHKLGMRTSTTSELIFEDMEVPEENILTKENSGFVRV
                     GRATLEWERTVLVSASLGSMQGMLDAGVRYAKERHQFGQPIFRFQAIQSKIAECRMKL
                     DAARLLIYKSASMKDKGLSAPIESSIAKLYATEAANQVMYDIGQIFGGYSFIHEYPIE
                     RAFRDARLGTIGAGTSEVMKAIISANI"
BASE COUNT          298 a          317 c          262 g          388 t
ORIGIN      
        1 gataatatta ggaagatatt tttccttctg ttcttccgtt ccgtgttctc tcaggggaag
       61 accgaaaaga ccgactgagg caccggagga aaaaaatgtg gaaccgcagt attcggaaag
      121 tatttcctgc gcaaggacag aaagctcata ccctgaatcc gaccctccgt acttttcctc
      181 atgcatcagg ccggtatagc ctatttcata aagtttctta taatgaaacc tgggaatcat
      241 tccttctttt tcagactctg cggtgtaggg ttctatttcc tttctgcaga aattccggaa
      301 agtatccaga aatgttctgt gctcttcgtt tatttcaaag ttcatattgt cctcaaatat
      361 tggcagagat gattgctttc atcacttccg atgtgcccgc accgattgtt ccgagtcttg
      421 catctctgaa tgctctttcg ataggatatt catgaatgaa ggaatatcct ccgaaaatct
      481 gtcctatgtc gtacatcacc tggttcgcag cttcggttgc atagagtttt gcaatggaag
      541 attcaatcgg tgcagaaagc cctttatcct tcatggatgc gcttttgtag ataagaagtc
      601 tggccgcatc gagtttcatc ctgcattctg caatcttgga ctgaattgcc tgaaagcgga
      661 agatgggctg accgaactga tgtctttcct ttgcataacg cactcctgca tcgagcattc
      721 cctgcatgga accgagtgaa gcggatacaa gaaccgttct ttcccattcg agagtggcac
      781 ggcccacgcg cacgaatcct gaattttcct tagtgagaat gttttcttcc ggaacttcca
      841 tatcttcaaa aatgagttcg cttgtagtgc ttgtcctcat tcccagcttg tgcagtttct
      901 ttcctgtttt gaatcccttg aaatttttct ccacgatgaa tacggaaaca cccatcgctc
      961 cttttgcctt gtccgtcact gccattacga tgaatacatc cccgatcggt ccgtttgtga
     1021 tgaacatttt tgaaccgttt ataatgtatt tatctccctt tttcacagcc cttgtctcca
     1081 tacttccggc agcatcactt cccgaagaag gttcagtaag tcctagggca gcagtccatt
     1141 caccgcttgc cagtctggga agatattttt tcttctgagc atcagttccg cagagaacaa
     1201 taggcataca gccgatgatg ctgtgtgcgc cccaggcaag agtcagtcct ccgtccattc
     1261 cgccc
//
LOCUS       DAOQGJ010001517         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88121_length_1264_cov_2.935484, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001517 DAOQGJ010000000
VERSION     DAOQGJ010001517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88121_length_1264_cov_2.935484"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(487..744)
                     /locus_tag="PK683_19250"
                     /pseudo
     CDS             complement(487..744)
                     /locus_tag="PK683_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588854.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
     gene            complement(812..1021)
                     /locus_tag="PK683_19255"
                     /pseudo
     CDS             complement(812..1021)
                     /locus_tag="PK683_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
     gene            complement(1133..1264)
                     /locus_tag="PK683_19260"
                     /pseudo
     CDS             complement(1133..1264)
                     /locus_tag="PK683_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
BASE COUNT          258 a          313 c          236 g          457 t
ORIGIN      
        1 cgcggttaat agctggctta ctctgcatca agctttcaat atggatgcaa tcttttccag
       61 tgccttttct gaatccgggc tgatgactgc ctttcctttc tgaaacgaga attttgtctt
      121 ttcaatatct tctgctgaaa aagattcttc cgtttttttt actccgggag attcctcaaa
      181 acggctttcg gcactgtcac tttttttgac agaactgcag gaaagcctga ttccgtaaat
      241 cagaagtcca atacatacag cagcaagacc gaatataagc cataccggta tttttgcttt
      301 ttcgctcacc ttctgctctt catagacaag cgcagcgatc gcaggacttg taggatgagc
      361 aggaggctgg acagtatgaa tatcctcaat aagcgggatt gtctcttcat tttttgttcc
      421 aggagattcc ttctttttcg gttcagagga tgtcttcttt tttgcagttg tttttttatc
      481 tgatgatttg ccagctgccc atttgcggat ttctgtcctg aaccatttgt ctgccgattc
      541 agtgaaagta taattattcc gtatatattc cactgtccgc ttttccacat cagtatactc
      601 gtttccgtct tttactgctt cgatcagttt ttttgcttca gcaagggaaa ttcgtccgtc
      661 tccttttcct gagacaaact cttccgccat atccagaagc tctttgtcat acttttttcc
      721 cttaatggtc ttatagtaac tcattttact ttactccttt ttttttggag atgctcttcc
      781 cttttttctt cgcgccggtt ttcgaggccg cccgtccggc tgcccatttg cggatttccg
      841 ttctgaacca gctgtcggca gactcagtga aagaataatt attccgtata tactccatag
      901 tcttcttttc agtgtcagta taactgttcc cgtcttttac agcactgatc agtttttttg
      961 catctgcaag agaaattctc ccgtcgcctt ttcctgaaac agacttgtcc gccatatcca
     1021 gaagtttcct gtcgtatttt ttgccattga cggttctgta ataaccctga tccgcagatt
     1081 ttttctcagc ggacgcactg cttccctttc tgacagcccc ggatacggaa gcccgtcctg
     1141 ctgcccattt gcggatttct gtcctgaacc atttgtctgc cgattcagtg aaggaaaagt
     1201 ttgctctgat gtactctaca gtccttttct ctatttctgt gtaatcattt ccgtctttga
     1261 cggc
//
LOCUS       DAOQGJ010001518         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88134_length_1264_cov_2.766749, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001518 DAOQGJ010000000
VERSION     DAOQGJ010001518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88134_length_1264_cov_2.766749"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..>1264
                     /locus_tag="PK683_19265"
     CDS             93..>1264
                     /locus_tag="PK683_19265"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNH10648.1"
                     /translation="MNLLLKILIIIFLTVGHLFSKDVSKNVTLAGSVPYKSEADSGLE
                     TAVDSALSNSLNQKDFAVTVSSGGRQAGLKSANQNNSIYYIETYYQRNKEKKILTVYA
                     LLYDPKTGNLVDVIRYTNDITESLGEEFRRFEDKYKEDEKRVIQKFSDTVSFSVLSNP
                     SKTEIRENIYDNLLSRPIGKEIQFSLAKEDKTGKSKEILSQLGEDEVVTASRKSQKIS
                     EAPSKVIVFTRDTIHERGYRTITELLQDVPGFDFNSFNDSGEYPTDMILRGISDVGQT
                     QILIMENGIIQNDIGNGWLRHMQFDTTLIDVERIEIILGPGSALYGANAYAGLINIIT
                     RKGKSLFGKKSSNIAAEARMQGGKNRTYMPEAMFAYKFQNDMIFQLSGRYYSTEGDR"
BASE COUNT          409 a          234 c          284 g          337 t
ORIGIN      
        1 atttacattt ttatgccttt atatacggaa gtgaatcaga agagacgtaa cggtctgatt
       61 caccgaaaaa aagctattac actgattaaa atatgaactt acttctgaaa atactgatta
      121 tcattttttt aactgtaggt catctttttt ccaaggacgt ttccaaaaat gtgactttgg
      181 ccggttctgt tccgtacaag tctgaagccg actcgggtct ggaaactgca gttgattcag
      241 ctctttcgaa ctctctcaat cagaaagatt ttgcagtgac agtcagttcc ggaggcagac
      301 aggcgggact aaaatctgca aatcagaaca attccatata ttatattgaa acttactatc
      361 aaagaaataa agaaaagaaa attcttacag tctatgcact gctgtatgat ccgaagacag
      421 gaaaccttgt ggatgtcata cggtatacaa atgatattac agagtctctg ggtgaggaat
      481 tccgaaggtt tgaagacaaa tacaaggagg atgaaaagag agtcattcag aagttttctg
      541 atacagtttc attctccgtc ctttccaatc cgtccaagac ggaaatccgc gaaaatatat
      601 atgacaatct tctttcccgg ccgatcggaa aagaaattca gttcagtctt gcaaaggaag
      661 acaagacagg gaagtccaag gaaatactga gtcagctggg cgaggacgag gttgtcactg
      721 catcgagaaa aagtcagaag atttcggaag caccttcaaa ggtgatcgta tttaccagag
      781 atacaattca tgaaagagga tacaggacaa tcactgaact tctgcaggat gtacccggct
      841 ttgattttaa ttcattcaat gattcggggg aatatcctac tgatatgatt ctgcggggta
      901 tttcggacgt cgggcagact cagatactga ttatggaaaa cggcatcatt cagaatgaca
      961 tcggaaacgg atggctcaga catatgcagt ttgacacaac tcttattgat gtggaaagga
     1021 ttgagattat tctcggtcca ggatctgcac tgtatggagc aaatgcttat gctggtctga
     1081 ttaatatcat caccagaaaa gggaaatcac tgttcggaaa aaaatcttcc aatattgcgg
     1141 ctgaggccag gatgcaggga ggaaaaaatc ggacctatat gccggaagct atgtttgcat
     1201 ataaatttca gaatgatatg atcttccagc tgtcaggaag gtactacagc acggaaggcg
     1261 accg
//
LOCUS       DAOQGJ010001519         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88156_length_1264_cov_2.400331, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001519 DAOQGJ010000000
VERSION     DAOQGJ010001519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88156_length_1264_cov_2.400331"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..767)
                     /locus_tag="PK683_19270"
     CDS             complement(63..767)
                     /locus_tag="PK683_19270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNH10649.1"
                     /translation="MKLPIFERRRRERIPRAILFVGILCILYPFINIYHLTSYYGIPL
                     KNPEMLLSRLNWMQKFLLALPLLSGTGILLRSIWGYIVFLLNALLLTGFNIYALIHFP
                     VRRNVFSLLEVILVTAVCLYLLRKNIGFGFIRREGGFRRASRKEIPVEVEIVGGGMSV
                     RLLTEDLSRKGFSVKWENCPLEINSETNVRIQLSEGLFSVRAGAVRKDGSTVGFAFRG
                     ISSEVRTAIRKEMNRE"
     gene            complement(919..>1264)
                     /locus_tag="PK683_19275"
     CDS             complement(919..>1264)
                     /locus_tag="PK683_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10650.1"
                     /translation="SILFSKGTSVLLINSDSISTLNLTSETAAIESPSGVRIIRNKFY
                     FQNYFYSYYCDNINTVQDVFPMQDMTGYLSIQSVSTKKVVISFFKSGRYLLYLETGDP
                     NFNPTDIYAVME"
BASE COUNT          390 a          275 c          224 g          375 t
ORIGIN      
        1 ccttccgaac cccaagccct gatccccgaa ctccaattat ttttcattac aaaacatcct
       61 ccctattccc tattcatttc cttccgaatt gccgttctga cctcagagga aatccctctg
      121 aatgcaaatc cgactgtgct tccgtctttc ctgactgctc ctgcacggac agaaaagaga
      181 ccttctgaaa gctgaatcct cacattggtc tctgaattga tttccagcgg acagttttcc
      241 catttgacag aaaatccttt ccgggaaaga tcctctgtca aaagacggac actcatccca
      301 cctccaacga tttctacttc caccggaatt tcttttctag aggctcgtct gaatcctcct
      361 tctctgcgaa taaatccgaa accaatgttc tttcttaaaa gatacaggca gactgctgtc
      421 acaagaatta cttctaaaag ggaaaataca tttcttctca ctgggaaatg aatcagtgca
      481 tagatattga atcctgtcag aagcagagca ttcagaagaa atacaatgta tccccagatt
      541 gaccgcagaa gaattcctgt tccggataag agaggcagtg caagcagaaa tttctgcatc
      601 caattgagtc tcgacagaag catttccgga tttttcagag gaattccgta atacgaagtc
      661 agatgataaa tatttataaa aggatacaga atgcatagaa tccccacaaa cagtatcgct
      721 ctgggaattc tttctcttct tcttctttca aatatgggta gtttcatgat attttctcct
      781 tttttggtat gcggaaagcg gggttttgaa gaaagggatc agagttcagg gcttggggtt
      841 cggagttcag ggaactgcag aaaaaagcag acgcttcgct gttcgttgaa ctccagttcg
      901 tttttatcat aaaaatcctc actccattac cgcataaata tctgtcggat tgaaattggg
      961 atctcctgtt tccagataaa gtagataccg cccagattta aaaaaagaga taactacctt
     1021 ttttgttgaa acagactgaa ttgataaata acctgtcata tcttgcattg gaaaaacgtc
     1081 ttgaactgta tttatattgt cacagtagta actgtaaaaa taattttgaa aataaaactt
     1141 atttctaata atccttaccc ctgatggaga ttcaatggca gctgtttcag acgtcaaatt
     1201 cagtgtgcta atactatctg aattaataag taaaacagat gttcctttag aaaaaagaat
     1261 acta
//
LOCUS       DAOQGJ010001520         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88268_length_1263_cov_2.853477, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001520 DAOQGJ010000000
VERSION     DAOQGJ010001520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88268_length_1263_cov_2.853477"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PK683_19280"
     CDS             complement(<1..1200)
                     /locus_tag="PK683_19280"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10651.1"
                     /translation="MLNQLKIRDFALIENLDLDFRKGMTSLTGESGSGKSLIFDAICT
                     VLGAKCNTLNIRSGAKRYQIEAEFSLKDLPAAAAYLEDRGIEAEDGKLVLRKELNQEG
                     KSRIQVNDALVPAQFLKELGSLLAEIHRQNEQISILERTKQLEILDTFAANQDLKKQY
                     QKSYTVYRTLKNRLIELETQKEDREKKKEIYEYQIQEIANAKLREDEEDKLIEEEKIL
                     IRSEKILQYLSGMAEELNGSEESALRHFTKILHHSERLGSIDPVYQPLTQDVQDIYYK
                     LRDLLTLINDNEEEIQFSLERMETVQNRLDQISRLKKKYGGKSVSELIQYAKTIENEL
                     EIFQSGMENLGNLKEELEKEKKKLCDVSIKLSQSRRSVIPMIESAISHELESLGMKDA
                     KLQVIMRW"
BASE COUNT          298 a          305 c          238 g          422 t
ORIGIN      
        1 ccatctcata atgacctgaa gttttgcatc cttcatccca aggctttcga gctcgtgaga
       61 aatggctgac tcaatcatag gaatcacaga tctcctgctc tgagaaagtt taatcgaaac
      121 atcgcagagt ttcttttttt ccttttccaa ttcttctttc agattgccca gattctccat
      181 ccctgactga aaaatctcca gttcattttc aatcgtcttt gcatactgaa tgagctcgga
      241 tacagatttt cctccgtatt ttttcttcag tcgggaaatc tgatcgagcc tgttctgcac
      301 agtctccatt ctctcaagtg aaaactgaat ttcttcttcg ttgtcgttga tgagagtcag
      361 aagatcacgg agcttataat aaatatcctg aacatcctgt gtgagaggct ggtatacagg
      421 atctatactt cctaatcttt ctgaatgatg gagtatttta gtaaaatgac gaagtgcgct
      481 ttcctccgat ccgttcagtt cctccgccat tccagagaga tactgaagga ttttttcgga
      541 ccgaatgaga attttttcct cttcgatgag cttatcctcc tcatcttctc tcagtttggc
      601 atttgcaatt tcctgaatct gatattcata aatttccttt ttcttctccc tgtcctcttt
      661 ctgagtttcc agttcaatca gacggttttt cagtgtacgg tacacagtat aggatttctg
      721 gtactgtttt ttcagatcct ggtttgcggc aaatgtatcc agaatttcca gctgtttcgt
      781 tctttccaga atggaaatct gctcattctg tctgtgaatt tccgcaagaa gacttccgag
      841 ctctttcaga aactgtgcag gaacaagtgc gtcattcacc tgaatccgcg acttcccctc
      901 ctgattgagt tcctttctga gaacaagttt cccgtcctct gcttcgatgc ccctgtcttc
      961 cagatatgca gctgcggccg gcagatcctt cagactgaat tccgcttcga tctgataacg
     1021 ttttgctcca gaccgaatgt tcagtgtatt gcattttgca cccagaacag tgcagatggc
     1081 gtcaaagatc agacttttac cggaaccgct ttctccggtt aacgaggtca ttccctttcg
     1141 gaagtccaga tccagatttt cgatgagtgc gaagtcgcga attttaagct gattcaacat
     1201 gcccgtctcc tgtttaatgt gaattcgaat cgaatccact atctgctttt aaaaaagcaa
     1261 gaa
//
LOCUS       DAOQGJ010001521         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88445_length_1262_cov_2.118476, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001521 DAOQGJ010000000
VERSION     DAOQGJ010001521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88445_length_1262_cov_2.118476"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PK683_19285"
     CDS             complement(<1..1051)
                     /locus_tag="PK683_19285"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10652.1"
                     /translation="MDQGKVGLIDIDNKRILQEWDSNLKDISNARLTDNDSKLITAHE
                     DGSIRIWEVSGKLLTSIIPEKNIGSIDLKDNGKKLFNSDESLKIWEAAAKDVQFKAQN
                     TNLLRAEIHKNSISILDTVQNKEISYLEGHSDKILSASFIPNQKLLISSSEDNSIKVW
                     DTSKGELKATIVPFKNGNQIIYTPDGRFDYTDKVVLEHISYRLPDRFVDLVDIQDQYY
                     TRGLLAKILSGKIREENIDGFVKALQIVPKIEHQYPEEDRLIPAGDKETEIGFNLKDQ
                     GTGIREVKVFVNGIETRVPEDIRKRLKQKSEEYDLTMNLPLVAGQNRVQITAYNNEGG
                     PNTKIIDLKGRRVIPG"
BASE COUNT          313 a          303 c          225 g          421 t
ORIGIN      
        1 gtccgggaat gacacgccgg cctttgagat caatgatctt tgtattcgga ccgccttcat
       61 tattgtatgc agtgatctgc acacggttct gtcctgccac aagaggaaga ttcatcgtca
      121 gatcatactc ttccgacttc tgcttcagac gtttcctgat atcctccggc acacgggtct
      181 caatcccgtt cacaaaaacc ttcacctcgc ggatgccagt tccctgatcc ttcagattaa
      241 aaccgatctc tgtctccttg tcccctgcag gaatcagcct gtcctcttca ggatactgat
      301 gctctatctt aggcacaatc tgcagtgcct tcacaaaccc gtcgatattc tcctctctga
      361 tcttccccga cagaatcttc gccagcagtc ccctagtata gtactgatcc tggatatcga
      421 ccagatccac aaatcggtca gggaggcggt aggagatatg ctccagcacg actttgtcag
      481 tataatcgaa ccgaccatcc ggagtataaa taatctgatt tccattttta aatggaacaa
      541 tcgtcgcctt gagttctccc ttggaagtat cccagacctt tatggaattg tcctctgagg
      601 aactgatcag gagtttctga ttcggaataa agcttgctga aagaatttta tcggaatgac
      661 cctccagata acttatttct ttattttgaa cagtatcgag tattgaaata gagtttttat
      721 gaatttctgc ccgtaacaaa tttgtgttct gcgctttgaa ctgaacgtct tttgccgctg
      781 cttcccaaat ctttaaagac tcatctgagt tgaataattt ttttccatta tcttttaagt
      841 ctatggaacc tatatttttt tccggaataa tgcttgtgag aagttttccc gatacttccc
      901 agatccgaat actgccgtct tcgtgggctg tgatcagctt ggaatcgttg tctgtgagcc
      961 ttgcattgga aatatccttt aaattagaat cccattcttg cagaattctc ttattgtcta
     1021 tatcgatcaa ccccactttt ccttgatcca aagctgccag cagtttactg ccgattataa
     1081 ccaatgattg atccaataaa atcaggggct tatctctttc tttttttgac ttagattcaa
     1141 aatcataacc ttctgtaatt tttcttaatg aatccttatt tttttgcaag aaatatgagt
     1201 aagtatttcc tccttgagca taaccacctt gtgttgtaaa ctctgagctt tctcctttga
     1261 ta
//
LOCUS       DAOQGJ010001522         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88457_length_1262_cov_1.968517, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001522 DAOQGJ010000000
VERSION     DAOQGJ010001522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88457_length_1262_cov_1.968517"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1262
                     /locus_tag="PK683_19290"
     CDS             <1..>1262
                     /locus_tag="PK683_19290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNH10653.1"
                     /translation="SIKKGVPRFNIVGLAGSAVREAPERVRIAIENSGYFFPLMNILV
                     NLSPAGIRKDSAFLDLAIAAGILSRTGQIFGEFDWEKILFLGELGLDGSIRPMKGISN
                     ILCSVDPKKFDKIILPSDNRYEAGFFQNLNLFPVQKLSGLEDILSGRQKKYEDISSTV
                     SERVRPYEISLFNDQIPAMENLIVSAAGKHHSLFIGSPGCGKTMLARFIPNLLPELST
                     EEFSEILRIRSVSDNENISFFSKERPFRSPHHTASDVSVIGGGKTQKLGEVTLAHNGV
                     LFLDELGEFKSPLIQALREPLEDEHVTVSRINYHVTYPASFLLVAASNPCPCGYSGSV
                     SRNCICSEIRIQKYLGKFSGPFLDRIDITAEMYSLEERDRSISVFPLEETYRRIENAF
                     LIQTERYRKLGCRFNGSLKGQDIDKYII"
BASE COUNT          367 a          276 c          285 g          334 t
ORIGIN      
        1 agcatcaaaa agggagttcc gcgattcaat atcgtaggtc ttgccggttc tgcagtcaga
       61 gaagcaccgg agagagtacg gatcgcaatc gaaaacagcg gttatttttt ccctctgatg
      121 aatattctgg ttaacctctc tccggctgga atcaggaagg attctgcatt tcttgatctg
      181 gcaatagctg caggaattct cagcaggaca gggcagatct tcggtgaatt tgactgggaa
      241 aagattcttt tcctgggtga acttggactg gacggaagca tccgcccgat gaaaggaata
      301 tcgaacatcc tctgttcagt cgacccgaaa aaatttgata aaatcatact tccatccgac
      361 aacagatatg aagctggatt ctttcagaat ctgaatctgt ttcccgttca gaagctttca
      421 gggctcgagg acattctttc aggaagacag aaaaaatatg aggatatatc ctccacagtt
      481 tcagagcgag tgcgtcctta cgaaatatct ctttttaatg atcagatccc tgcaatggaa
      541 aatctgatcg tatcggcggc aggaaagcat cacagtcttt tcatcgggag tccaggatgc
      601 ggaaagacaa tgctcgccag attcattccg aatcttctcc ccgaactgag cacagaggaa
      661 ttttccgaga tactcagaat caggtcagta tccgataatg aaaatatatc ttttttttcc
      721 aaagaaaggc ctttccgttc tcctcatcac actgcatccg atgtatctgt gatcggcgga
      781 ggaaagactc agaaactcgg tgaagtgact ctggctcata acggagtgct ttttctggat
      841 gaactgggag aattcaagtc tcctctgatc caggcactca gagaaccttt ggaagatgaa
      901 catgtcacag tgtccagaat caactatcat gtcacctacc ctgcttcctt tcttcttgta
      961 gccgcatcca atccctgtcc ctgcggatac agcggatctg tctcgagaaa ctgcatctgt
     1021 tctgaaatta gaattcagaa atatctggga aagttttccg gacctttcct ggacaggata
     1081 gacatcacag cggaaatgta cagccttgaa gagagagaca gaagcatttc ggtttttcct
     1141 ctggaagaga cttaccgcag aattgaaaat gcatttctga ttcagacaga aaggtacaga
     1201 aaacttgggt gcagattcaa cgggtcactg aaaggacagg atatagataa atatattata
     1261 at
//
LOCUS       DAOQGJ010001523         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88493_length_1261_cov_4.655058, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001523 DAOQGJ010000000
VERSION     DAOQGJ010001523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88493_length_1261_cov_4.655058"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1261)
                     /locus_tag="PK683_19295"
     CDS             complement(<1..>1261)
                     /locus_tag="PK683_19295"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH10654.1"
                     /translation="SVPEKPSSRQCDNEPIHLINELQDFGILLILSLEEDRFLACSDN
                     TFEYAGIKPEELMMISPGQFLENYDKIKININDIIVNKKQQEYKSIFHFRNELYFATF
                     RKSENFFICEIERETYSSALESSIDLLVKINELQAVQDLQSLFDFTVSEVRKIAESDR
                     VVLYRFKEDFSGEVVAESNNGNLPSLMGFHFPSTDIPLPARAMYTKNPIRMIKLNQCR
                     PVRLLKTNLYKEYSFDLGASSLRAPHRHHIEYLNNMGIVSSMSVSLSKDGQLWGLLIC
                     HCLEERVPSLSVRNFLSIYSKTVYLQIDALNRNNDSLAELRIQELFLNIIKKINTSPF
                     EKIMDVFRQESRPLLQEFQSDGLYIQVQDQSFRYGSLPSENILQKIKSVLSEKFPGSV
                     FYTDDLKETASEDLSDTEHANGVMSIPL"
BASE COUNT          351 a          256 c          261 g          393 t
ORIGIN      
        1 aagaggaatg gacatcaccc cgttggcgtg ctcagtatct gataggtctt ccgatgcagt
       61 ttctttcaga tcatctgtat agaatacaga tcctggaaat ttctcagaaa gaactgattt
      121 gattttttgc agtatgtttt cagacggtaa tgaaccgtat ctgaatgact ggtcctgtac
      181 ctgtatatac agtccgtcag actgaaactc ctgcagcagt ggtctgcttt cctgtctgaa
      241 cacatccatt attttttcaa aaggggaagt attgatcttt ttgataatgt tcagaaataa
      301 ttcctgaatc ctcagttctg caagtgaatc gttgtttctg ttcagagcat caatctgcaa
      361 ataaacagtc ttgctgtata tggataagaa gtttcggaca gataaactgg gaactctttc
      421 ctcaagacag tgacagatca gaagccccca gagctgaccg tcctttgaca gtgaaacgga
      481 cattgaactg acaatcccca tattgttcag atattcaatg tgatgcctat gaggtgccct
      541 gagtgaggat gcaccgagat caaatgaata ttccttatac aggttggttt tcagaaggcg
      601 tacaggcctg cactgattca gtttaatcat tcgaatagga tttttagtgt acattgctct
      661 ggcaggaaga ggtatatctg tggaaggaaa atgaaatccc atcagggagg ggagattacc
      721 gttgtttgat tctgccacta cttccccgga aaaatcttcc ttgaatctgt atagaacaac
      781 cctgtccgac tctgcaattt ttctcacttc actgactgtg aaatcaaaaa gactctgcag
      841 atcctgcact gcctgcagtt cattgatctt taccagaaga tcaattgatg attccagtgc
      901 actggagtat gtttcccgtt caatttcgca gataaaaaag ttttcgctct ttcggaaggt
      961 tgcaaaataa agttcatttc ggaaatgaaa aatggatttg tattcctgct gctttttatt
     1021 tacaataata tcatttatat taatttttat cttatcgtag ttttccagaa actgaccagg
     1081 agatatcatc atcagctcct ccggctttat gcctgcatat tcaaacgtgt tgtcgctgca
     1141 tgcaagaaac cggtcttctt caagagacag gatcaggagt attccgaaat cctgcagttc
     1201 attgattaaa tgaatcggtt cgttgtcgca ctgccgtgaa gaaggttttt ccggcacgga
     1261 c
//
LOCUS       DAOQGJ010001524         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88505_length_1261_cov_3.838308, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001524 DAOQGJ010000000
VERSION     DAOQGJ010001524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88505_length_1261_cov_3.838308"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PK683_19300"
     CDS             complement(<1..900)
                     /locus_tag="PK683_19300"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH10655.1"
                     /translation="MTAPYLSENETERLENLRSFQILDTYPEEEYDSIARLVSLICEV
                     PTALISLVDSDRQWFKSKINMNFNETPRDISFCGHAILDNSVFIIENAAEDIRFRENP
                     LVTGYPKIRFYAGAPLVTNEGYNIGTLCAIDYIPKRLSPEQTESLVLLSRQVVNLFEA
                     RKLALTYESAIHEIEKNRLNHLKLESRYYSIVSSMKEGVIQTDVNGIILSCNISACRI
                     LGMNAQELEGRHFFFQDTEFLKGDSTVLKEEDHPVSMVLKNRTGYEDIVIGLKLPNGR
                     LLWINVNSEPILENDQFAGAVISF"
     gene            complement(902..>1261)
                     /locus_tag="PK683_19305"
     CDS             complement(902..>1261)
                     /locus_tag="PK683_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10656.1"
                     /translation="ITFKVRIILENFYKKNMDLRVLDRIPAEDEKENIRITLDLKANS
                     LSRDEQYVQYERPKGILRWDTALSGESSAGKAKVIEYSYKLEHDRNLGVTAPSAQKTD
                     QMRLEFDEIQKLKHNRK"
BASE COUNT          310 a          275 c          259 g          417 t
ORIGIN      
        1 aaatgaaatg accgctcctg caaactgatc attttccagg atgggttctg agtttacatt
       61 gatccaaagc agtcttccgt tcggaagttt aagaccgatt acaatatcct catatcctgt
      121 ccggtttttc agaaccatgg atacagggtg atcttcttct ttcagaacag tgctgtcacc
      181 cttcaaaaat tcggtgtctt gaaagaaaaa atgcctgcct tccagttcct gtgcattcat
      241 gccaagtatc ctgcaggcac tgatattgca ggataaaatg attccgttga catcggtctg
      301 aatcactcct tccttcatgg aagatacaat ggaatagtac ctgctttcga gtttgagatg
      361 gttcagacgg tttttttcta tctcatgaat tgccgattca taagtgagtg ccagttttct
      421 ggcttcaaaa agattgacaa cctgtctgga aagaagaacc agagattcgg tctgttctgg
      481 actcagtctt ttcgggatgt agtcaatcgc acagagcgta ccgatattgt acccttcgtt
      541 cgttacaaga ggtgctcccg catagaatct gatttttgga taccctgtca caagaggatt
      601 ttcgcggaat cttatgtcct ctgctgcgtt ttctataatg aaaacagaat tgtccaatat
      661 tgcgtgtccg cagaaggata tgtctctggg agtctcattg aaattcatat tgatctttga
      721 tttaaaccac tgccggtctg aatcaaccag tgaaatcagg gctgtcggca cttcacagat
      781 cagtgagaca agtcttgcaa ttgaatcata ttcttcttcc ggatatgtgt cgaggatctg
      841 gaaacttctc agattttcca gtctctccgt ctcattttcc gaaagatagg gtgcggtcat
      901 attattttct gttatgcttc agtttctgga tttcatcaaa ttcaagtctc atctgatctg
      961 ttttctgagc tgaaggagct gtgactccca gatttctgtc atgttcaagc ttgtaggaat
     1021 attcgattac cttcgctttt cctgcagaac tttctcctga aagtgccgta tcccatctga
     1081 ggattccttt gggtctttca tactgaacat actgttcgtc tctgctcagt gaatttgcct
     1141 tcagatccag agtgatgcga atgttttcct tctcgtcttc agcagggatt ctgtcgagca
     1201 ctctcagatc catattcttc ttgtagaaat tttcaaggat gatccgcact ttaaaagtga
     1261 t
//
LOCUS       DAOQGJ010001525         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88543_length_1261_cov_2.666667, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001525 DAOQGJ010000000
VERSION     DAOQGJ010001525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88543_length_1261_cov_2.666667"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1149)
                     /locus_tag="PK683_19310"
     CDS             complement(112..1149)
                     /locus_tag="PK683_19310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNH10657.1"
                     /translation="MFKKLKNIFICLLVLSAAVHVFDLIGQPGTPVKRFKLLAGGDIM
                     FDWGLRQVMKSKGKFVPVDNLLPLFIEADFSMANLETPVSETAEDIDRLKSYVFNAKP
                     SEMEVLNHLGIDLVFLANNHTMDYGVKGIEETFKHLKKYNLHHVGAGRNLKESFLPFT
                     VAGLNARIYSVSQIGETRLFATDKKPGAAPFVMSTLKSIPDKTKLNILSVHWGQEYNP
                     EPMPSQITNAHELINAGFRIIIGHHPHIPQGIEKYKNGIIFYSLGNFIFGSRNPYLNH
                     NMIVMLHFENENPVLAEIIPVFGKFQEAEHIFGPVKTDEAESFLQEIAVLSLKLNTKL
                     EIRNGRGYIKL"
BASE COUNT          359 a          285 c          229 g          388 t
ORIGIN      
        1 tggccaggtg cacaacatac gatacatctg aaccgggaat cttctgatcc ttatgtgcaa
       61 ttcctgcaaa tgcaattgct ttctgatatg catcctgaat tccgtacgga ttcaaagttt
      121 tatgtatcct cttccattcc tgatttccag ctttgtattc agcttcagag aaaggactgc
      181 aatctcctgc agaaaggact ctgcctcatc cgtttttaca ggtccaaaaa tatgctctgc
      241 ctcctgaaat tttccgaata cgggaatgat ctcggcaagt acaggatttt cattttcgaa
      301 atgaagcatc acaatcatat tatgattcag gtaaggattt ctgcttccaa aaatgaaatt
      361 gcccaatgaa taaaatatga ttccattttt gtatttttct atcccctgcg gaatatgagg
      421 atgatgacct atgataattc tgaatcctgc attgatcagc tcatgcgcat ttgtaatctg
      481 agagggcatc ggttccggat tgtactcctg tccccagtgc actgaaagta tgttcagctt
      541 agttttgtca gggatgctct taagcgtgct catgacgaaa ggagctgcac ccggtttttt
      601 gtctgttgca aaaagccttg tctcccctat ctgactcaca ctgtaaattc ttgcattcag
      661 accggccaca gtaaaaggca gaaaggattc cttcagattt cttcctgcgc ctacatgatg
      721 aagattgtat ttcttcaaat gcttgaatgt ctcctcaatc cccttcacac cgtaatccat
      781 ggtatgatta tttgccagaa aaacaagatc gatccccaga tgattcagaa cttccatttc
      841 agaaggtttt gcattgaaca cataggactt cagtctgtcg atgtcttctg ctgtttcaga
      901 aacaggagtt tccagatttg ccatggaaaa atcagcttca atgaaaagag gaagcagatt
      961 gtcgacggga acaaatttcc ccttcgactt cattacctgc ctgagtcccc agtcgaacat
     1021 aatatctcct cctgcaagaa gcttgaatct tttcacagga gttccgggct gaccgatcaa
     1081 atcaaaaacg tgaactgccg ccgaaagaac aagaaggcag ataaaaatat tttttaattt
     1141 tttaaacata taatatattc cagaaaactg aataattcag tcgaaagaat cttttccctc
     1201 agaatcactg aaattttccg cccattcaat tccgttctcc ttgaattttt tcagaagttt
     1261 c
//
LOCUS       DAOQGJ010001526         1260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88669_length_1260_cov_2.868880, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001526 DAOQGJ010000000
VERSION     DAOQGJ010001526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88669_length_1260_cov_2.868880"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PK683_19315"
     CDS             complement(<1..547)
                     /locus_tag="PK683_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNH10658.1"
                     /translation="MSKFIDEVPITVIAGHGGPGAVSFRHEKFAEFGGPDGGEGGYGG
                     NVILKSDLNLQTLDKFIPLKHYSAGAGSHGEGKNRSGKKGEDIILKVPVGTQVTDIET
                     EDVLFDFVKEDLEYIAAKGGRGGKGNAFFKSSTHQAPRFAQDGEEGESKDILLTLKLL
                     ADVGLVGLPNAGKSTLLSRITH"
     gene            complement(540..1178)
                     /locus_tag="PK683_19320"
     CDS             complement(540..1178)
                     /locus_tag="PK683_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10659.1"
                     /translation="MGDSIKDLSFLEEDEVSYFYSLLTEEITDFDCGTLCKGDNEGIP
                     FCCSVENAVPFLYSAEFSYLKKRTDLWKVWKPVTEQQKELKKSNEDRSTIFCECKGVQ
                     YCERENRSISCRTFPMEPYIDRRGVIVGLVFMKEFTGGCPLTGKPEKIRQEYIDSSFL
                     FWEKMLLRRPLEYDTYKKSSKAYRISRTKSGKNFPILYPSHLKDKTYLRKYV"
BASE COUNT          333 a          318 c          246 g          363 t
ORIGIN      
        1 catgagtgat tctgctgagc agtgtagact tgcctgcatt agggagaccg acgagaccca
       61 cgtcggccag aagtttcaga gtgagcagaa tgtccttgga ctcaccctcc tctccatcct
      121 gcgcaaatct aggagcctga tgagtggagg atttaaaaaa agcatttcct tttccccctc
      181 ttcctccctt tgcggcaatg tattcaaggt cttccttgac gaaatcaaac agaacatctt
      241 ccgtttcaat atctgtcacc tgggttccga ctggaacctt cagaatgatg tcctcacctt
      301 ttttgcccga tctgtttttc ccttctccgt gagagcctgc acctgctgaa taatgcttca
      361 gaggaatgaa cttgtccaga gtctgcagat tcagatcaga cttcagaatt acatttccgc
      421 catatccccc ctcgcctccg tccggtcccc caaactctgc gaacttttca tgacggaaag
      481 aaacagcacc gggaccgccg tggcctgcaa tgacagtaat tggaacctca tcaataaatt
      541 tagacatatt tcctcagata agttttatcc ttcagatgag aagggtaaag gattggaaaa
      601 ttctttcctg acttagtacg ggatattctg tatgctttgg aggatttttt atatgtatca
      661 tattccaaag gtcttctcag aagcatcttt tcccagaaga gaaaactgga atcaatgtat
      721 tcctgacgga ttttctcagg ctttcctgtc agaggacagc ctccggtaaa ctctttcata
      781 aacacgagtc ctacaatcac gcctctcctg tcaatgtaag gctccatagg aaaggttctg
      841 cagctgatgg atctgttttc cctttcacag tactgcacgc ccttgcattc acagaatatt
      901 gtagaacggt cttcattgct ttttttgagt tctttctgct gctctgtaac aggtttccag
      961 accttccaga ggtccgtcct tttttttaaa taactgaatt ctgcactgta caaaaaggga
     1021 accgcatttt cgacggaaca gcagaaagga attccctcgt tgtcaccctt gcagagcgtc
     1081 ccgcagtcaa aatccgtgat ttcctcagtc agaagcgagt agaaataact tacctcatct
     1141 tcttcgagga atgacaagtc tttaatggaa tctcccatag ttccagtctg caaacaagtc
     1201 ttctgcaaac aaccaagaaa aatgtactgg aaaaatactc agttatcctg aaaactgcca
//
LOCUS       DAOQGJ010001527         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88770_length_1259_cov_3.048173, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001527 DAOQGJ010000000
VERSION     DAOQGJ010001527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88770_length_1259_cov_3.048173"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1259
                     /locus_tag="PK683_19325"
     CDS             <1..>1259
                     /locus_tag="PK683_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH10660.1"
                     /translation="LSLIMGQAMYQTPLFNGKGYLAAGGKVGYLDKTIGATGLIPEGI
                     RLPQYTSSNVKFIYDINADHQISFISLTASDNFVANFSNKPNNDPTKDPLAAVAGARV
                     AAGQGYRTTALRHIWTVNSKITNRFTLINYDPFAKVNVKFGTIESDFIARAPYTGLRQ
                     DMYINAGNMLKIDLGTEYRLFSYNLSGFNVQQTDPNNLSPNPYNTANPDFQKKDITQK
                     TPTNYANAYTTLHFTFGKLKIEPGLRYDYVGYSKQGVLGPRGTVSYKLEGFLKGTTFF
                     AGAGDYYRYPFFSDAISKESGNPDIKFEKARKYGGGIEQDISDILSVKTEMFKQEFTD
                     LIVSDPYISAPYGVNPDKTQLLTQPIVANRALNFSNKGTGWARGYELYIKKNPKPGVK
                     DWYGWISYTWSQNFRNTNIYNSNADPS"
BASE COUNT          405 a          273 c          267 g          314 t
ORIGIN      
        1 atctttctct gattatgggt caggcaatgt accagactcc ccttttcaac ggaaaaggct
       61 atcttgcagc gggaggaaaa gtcggatatc tggataaaac aataggagca acaggactga
      121 ttcctgaggg aatcagactt cctcagtaca caagctcaaa cgtaaaattc atatatgata
      181 taaatgcaga tcatcagatc tctttcattt cgctgactgc atccgacaac tttgtcgcca
      241 acttttcaaa caagccgaac aatgatccaa caaaggatcc cttggctgct gtagcaggag
      301 caagagttgc cgcaggtcag gggtacagaa caactgcact ccgtcatatc tggacagtga
      361 attcaaagat cacaaacaga ttcactctga tcaattatga tccattcgca aaggtaaacg
      421 taaaattcgg aacaatcgaa tcggatttca ttgcccgggc gccttacact ggtctccgtc
      481 aggatatgta catcaatgcg ggaaatatgc ttaagattga ccttggaaca gaatacaggc
      541 tgttcagtta caatctgagc ggctttaatg ttcagcagac tgatcccaac aatctttctc
      601 caaaccctta caatacagca aacccggatt ttcagaaaaa ggacatcacg cagaagactc
      661 ctacaaatta tgccaacgca tacacaactc tgcatttcac ttttggaaaa ctgaaaattg
      721 agcccggctt gagatatgat tatgtaggct attctaaaca gggagtgctt ggtcctagag
      781 gaacggtttc ctacaaactg gaaggcttcc tgaaaggtac aacattcttt gcaggggcag
      841 gggactatta caggtatccc tttttcagcg atgccatttc aaaagaatca ggaaatcctg
      901 acatcaagtt tgaaaaggcg agaaaatatg gcggagggat cgagcaggat atttcagata
      961 ttctgtctgt aaaaacagaa atgtttaagc aggagtttac tgatttgata gtaagtgatc
     1021 catatatttc tgctccttac ggtgtcaatc ctgacaagac gcagcttctc actcagccga
     1081 tcgtcgcaaa cagagcattg aatttttcca ataaaggaac cggatgggca agaggatatg
     1141 aactctacat aaagaaaaat cccaagccgg gagtcaagga ctggtacgga tggatatcct
     1201 atacctggtc gcagaatttc agaaacacca atatttacaa ttcgaatgca gatccgagt
//
LOCUS       DAOQGJ010001528         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88882_length_1258_cov_3.294264, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001528 DAOQGJ010000000
VERSION     DAOQGJ010001528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88882_length_1258_cov_3.294264"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..743)
                     /locus_tag="PK683_19330"
     CDS             complement(81..743)
                     /locus_tag="PK683_19330"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNH10661.1"
                     /translation="MAKNSILPFALACLFLFGLANCNPADKKDTPPPKEDKVEPAVEK
                     PKEDKPAEEPPVKTTERDLLDTGIDALINQFNEKLKGVRYPDGSSIEGFEYKKWEIPN
                     RKDFVKWVKVSGAIVKEAFEKLPEGVKLEITGHADTKGPEEKEGKKLGNIFYSTKRAE
                     SVRDSLVKLGFPEKRMMTRGAGSSETIPGIDGTSAKNRRVTFKLLNTGSEKPAEEADQ
                     GK"
     gene            complement(835..>1258)
                     /locus_tag="PK683_19335"
     CDS             complement(835..>1258)
                     /locus_tag="PK683_19335"
                     /inference="COORDINATES: protein motif:HMM:NF033171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative lipoprotein"
                     /protein_id="HNH10662.1"
                     /translation="CSTLNSISNASQSLNSISTSVNSISRAFDSVSSISNSVGSISGS
                     SSKKAAASALYHRDVRDLTYLSVKNGQDLDFTSELNLISQGHGFLNWQDKSETYLGIG
                     EGLRKAGVNEPEFKNWMSGLKLDISRSSLLSNGFYGRI"
BASE COUNT          339 a          280 c          241 g          398 t
ORIGIN      
        1 gggacacctg gtttgattat cggaaaggaa attgagaaaa aaaacaaaaa accccgcacc
       61 gagcggggtt ataaaatgag ttacttgcct tggtctgctt cttcagcagg cttttcactg
      121 ccagtattca aaagtttaaa agtcaccctt ctgttcttag ccgatgttcc atcaatgcct
      181 ggaattgttt ctgaagaacc tgctcctcta gtcatcattc tcttttccgg gaaacccagt
      241 tttacaagag aatctcttac agattcagca cgtttagtag agtaaaaaat atttcccaat
      301 tttttacctt ctttttcttc aggtcccttt gtatcagcgt gaccggtaat ttccagtttt
      361 acgccttcag gaagtttttc aaatgcttct tttacgatag caccggaaac ttttacccat
      421 tttacaaaat cctttctgtt agggatttcc cattttttat attcgaatcc ttctatggag
      481 cttccgtcag gataacgaac ccctttcagt ttttcgttga attggttgat taaagcatca
      541 atgccagtat ccagaagatc tctctccgtc gtcttgacag ggggttcttc agccggttta
      601 tcttctttcg gtttctcaac tgcaggctcc accttatctt ccttaggggg aggagtgtct
      661 tttttgtctg ccggattaca attagccagt ccaaacaaaa acaggcaggc aagtgcaaaa
      721 ggcagtattg aattcttagc catagtattg cttccttttt tcatgtcgtt gtactcttac
      781 tatcattaca gaataaaaaa gaaacctttt tttacaagta aatttcaggg agattcagat
      841 tcgtccataa aaaccattgg acaaaagact gctcctgctg atatcaagct taagccctga
      901 catccagttt ttaaattccg gttcattgac tcccgccttt ctcagcccct ctccaattcc
      961 aagatatgtt tccgatttat cctgccagtt gagaaatcca tgaccctggg aaatcaggtt
     1021 cagctcggaa gtaaaatcca gatcctgccc gttttttaca gagaggtaag tcagatccct
     1081 cacatccctg tgatacagtg ctgaagcagc tgcctttttt gaggaagatc cgctgatcga
     1141 tcccactgag ttggaaatgc tggaaactga atcaaatgct cttgaaatgg aattgacaga
     1201 agtggaaatg gaattgagcg actgagacgc atttgagatg gaattcaatg tggaacat
//
LOCUS       DAOQGJ010001529         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_88912_length_1258_cov_2.577722, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001529 DAOQGJ010000000
VERSION     DAOQGJ010001529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88912_length_1258_cov_2.577722"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PK683_19340"
     CDS             <1..517
                     /locus_tag="PK683_19340"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNH10663.1"
                     /translation="ISAQMLGAFAGACLIYLNYHDAFAEFETKNSILRGYETSIRTAG
                     IFATYPPPFISNLNAFFDQFLGTALLIILILTLTDNQNKVIDLNISPLLVGLGVVAIG
                     MSFGGNAGYAINPARDFGPRLASSIFGWGTVVFTAHNYYFWIPIIAPILGGIAGSYLY
                     DFFLGSRLDKN"
     gene            complement(522..>1258)
                     /locus_tag="PK683_19345"
     CDS             complement(522..>1258)
                     /locus_tag="PK683_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH10664.1"
                     /translation="RQPEAEEEVRDALRALFACPVHSIGFTEKPSFLSEVRNSFPERI
                     EENIFYCGFHSEKSYGAFSYLIFREDGNILVDSPRFTKPLIRNLEKMGGVRYMFLTHQ
                     DDVADHEKFHLHFQCERIIHSLDRNGMNAEIILQGSDDSKFSDDILFIPVPGHTRGSV
                     CLLYKDRYLFSGDHLAYSDKRNHLVGFRDACWYSRKELYLSMKKLQSYSFQYVLPGHG
                     KSYRAGTAEEMKEQMELCVRFLSEKF"
BASE COUNT          355 a          286 c          243 g          374 t
ORIGIN      
        1 catatccgct cagatgttag gggcattcgc gggggcctgt ctgatatatt taaattatca
       61 tgatgctttc gcagaatttg aaactaagaa ttccatcctg cgaggatatg aaacaagcat
      121 aagaacagca ggaatatttg ccacctaccc tcctccattc atttccaatc tcaatgcgtt
      181 ctttgatcag tttctcggaa ccgcacttct gattatactg attctcacac tgacggacaa
      241 ccagaacaaa gtaattgatc tgaatatctc acctctttta gtcggtctcg gagttgtcgc
      301 aatcggtatg agtttcggag gaaatgcagg gtatgcaatc aatccggcaa gagattttgg
      361 acccagactc gcctcctcca ttttcgggtg gggaacagtg gttttcactg ctcacaatta
      421 ttatttctgg attccgatta tagcacctat tctgggagga atcgcagggt catatctata
      481 cgattttttt cttggatcaa gactggataa aaactgattc gttaaaattt ttcagacaga
      541 aatcttacac agagttccat ctgttctttc atttcttcag cagttccagc cctatatgat
      601 tttccgtgac caggaaggac atactgaaaa ctgtatgact ggagtttttt cattgagaga
      661 taaagttcct ttctggaata ccagcatgca tcccgaaaac cgaccagatg atttcgttta
      721 tccgaatatg ccaaatgatc gccgctgaaa agatatctgt ccttatacag cagacagacg
      781 cttcctcttg tatgacccgg cacaggtatg aataaaatat catcggagaa ttttgagtca
      841 tccgatccct gaagtattat ctcggcattc attccattcc tgtcgagcga atgaataatc
      901 cgttcacatt gaaaatgaag atgaaatttt tcgtgatctg ccacatcatc ctgatgtgtc
      961 agaaacatat acctcactcc tcccatcttt tccagatttc tgatcagtgg ttttgtaaat
     1021 cggggagaat cgacaaggat attgccatcc tctcgaaaga taagatacga aaatgcaccg
     1081 taagattttt cagaatgaaa tccgcaataa aatatattct cctcaatcct ttccggaaaa
     1141 ctgtttctca cttctgaaag aaaagacggc ttttccgtaa aaccgataga atgaactggg
     1201 caggcgaaca gtgccctgag tgcatccctg acctcctctt ccgcttcagg ctgtctgc
//
LOCUS       DAOQGJ010001530         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89016_length_1257_cov_2.778702, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001530 DAOQGJ010000000
VERSION     DAOQGJ010001530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89016_length_1257_cov_2.778702"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..841)
                     /locus_tag="PK683_19350"
     CDS             complement(92..841)
                     /locus_tag="PK683_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH10665.1"
                     /translation="MKIWKIIILLSFIPGLIFFPEPGRISLNQSDCNVSFSHFFGCDS
                     LGRDVYSMYSYGILLNFFIAIPARILSLALSLAVLSFRFSGIRTVKMLTDSFASVFLS
                     VPSILVGLLIAVSFGKSLPVFLFALAASDWALSYETLSGKLRETENGGFITVSFLFGA
                     DRFYVFRKHLLPVLLEYSKTLFVIGLPGVIMTASLFSYLGLDFGVSFFGPGLGEQISF
                     SKDYFFRNPFSTAVPVFGILFSILLFRENHK"
     gene            complement(838..>1257)
                     /locus_tag="PK683_19355"
     CDS             complement(838..>1257)
                     /locus_tag="PK683_19355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH10666.1"
                     /translation="RWDISYLILPGLALGSRTFARVCLFTEAEVRKEKESAYAVFLFC
                     RGMNRNTVFFKYIFWKIFPLISVIILLDFGSLLSGAVIVEEIYFYPGIGKSVYHAIRN
                     FDGNLLKGILFYTGIIFYSSARLSRYLQSYSSGAWSR"
BASE COUNT          448 a          252 c          270 g          287 t
ORIGIN      
        1 ttcatctgtg caggaatgta ctgttcgctg aatttgaaca tagagtcttc agcgttcaat
       61 gaaacagctg caaggataag aaataataat ttcatttatg attctccctg aataaaagta
      121 tgctgaaaag aattccaaaa acaggaactg cagtgctgaa aggatttctg aaaaaataat
      181 ccttggaaaa cgaaatctgc tctcccagac caggcccgaa aaatgaaaca ccgaaatcga
      241 gtcccagata actgaagaga gatgcagtca ttataactcc ggggaggccg atcacaaaca
      301 gtgttttgga gtattccaac agaacaggca gaagatgttt tctgaaaaca taaaatctgt
      361 ctgcaccgaa aagaaaggaa actgtaatga accctccgtt ttccgtttct ctgagttttc
      421 cgctcagagt ctcataggaa agagcccagt cagaagcagc aagcgcaaaa agaaaaacag
      481 ggagtgactt tccaaatgat acagcaatca gaagaccgac aagaattgac ggaacagaaa
      541 ggaaaacgga tgcaaacgaa tcggtaagca tctttacagt tctgattccg gaaaaacgga
      601 aagaaagcac tgcaagactt aatgcaagac tcagaattct tgcgggaatt gcgataaaaa
      661 aattcagaag aattccgtaa gaatacatag aataaacgtc cctgcccaga gagtcgcacc
      721 cgaaaaaatg gctgaaggaa acattacagt cggactgatt cagactgatt cggccaggct
      781 ctggaaaaaa aatgagtccc ggaatgaagc tgagaaggat aatgattttc cagatcttca
      841 tcggctccag gctccggaag aataggactg cagatacctg gaaagtctgg cagacgaata
      901 gaaaatgatt ccggtataaa acagaattcc tttcagaaga tttccgtcaa aattccttat
      961 tgcatggtag actgattttc cgatgcccgg ataaaagtaa atttcttcaa caatcactgc
     1021 acctgaaaga agcgaaccaa aatccagtag aatgatgacc gagatgaggg gaaatatttt
     1081 ccagaagata tatttgaaaa agactgtgtt cctgttcatt cctctgcaga aaaggaatac
     1141 tgcgtaagct gactcttttt ctttcctgac ctcggcctct gtgaaaagac agacccttgc
     1201 aaaggttctt gatccaagag ccaggcccgg aaggataaga tatgatatat cccatct
//
LOCUS       DAOQGJ010001531         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89083_length_1257_cov_1.599002, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001531 DAOQGJ010000000
VERSION     DAOQGJ010001531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89083_length_1257_cov_1.599002"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..655
                     /locus_tag="PK683_19360"
     CDS             95..655
                     /locus_tag="PK683_19360"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNH10667.1"
                     /translation="MKRLIFMGPPGAGKGTQAKVISVEFKIPQISTGDILRSSIQAGT
                     EMGLKAKKYMDAGDLVPDEVVIGIIRDRLKESDCVNGYILDGFPRTTEQAKALDQLLQ
                     SIGSKLDAVVNIAVPDKELVSRLLERAKIEGRADDNEETIKNRLSNYNQKTFPLLDYY
                     RSTGLLKEIDGVGKPEEITALIKKGI"
     gene            660..878
                     /gene="infA"
                     /locus_tag="PK683_19365"
     CDS             660..878
                     /gene="infA"
                     /locus_tag="PK683_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNH10668.1"
                     /translation="MAKEEAITVDGTVMEPLPNAMFRVELENGHKILAHISGKMRMHY
                     IRILPGDKVTVELSPYDLTKGRITYRKK"
     gene            886..999
                     /gene="rpmJ"
                     /locus_tag="PK683_19370"
     CDS             886..999
                     /gene="rpmJ"
                     /locus_tag="PK683_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNH10669.1"
                     /translation="MKVRASVKKICSSCKVIRRNNVIRVICTNPKHKQRQK"
     gene            1000..>1257
                     /gene="rpsM"
                     /locus_tag="PK683_19375"
     CDS             1000..>1257
                     /gene="rpsM"
                     /locus_tag="PK683_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNH10670.1"
                     /translation="MARFAGIDLPREKRIVVGLTYIYGIGRSSSEKILKKAGVDESVR
                     VKSLTEAQENAIRKVLEEGYALEGDLRSETQLNIKRLMSSYF"
BASE COUNT          449 a          199 c          298 g          311 t
ORIGIN      
        1 aatggacact ctgaagcaga ttgaagcaca gctgacaatg agaaattatg aaggatttat
       61 gaaaaaatcc aagatcaagg ggaggtaaga gtttatgaaa agattaatat ttatgggtcc
      121 tcccggtgca gggaaaggta cacaggcaaa agtaatatct gttgaattca aaattcctca
      181 gatatctact ggagatattt tgagatcatc cattcaggct ggaactgaga tgggtctgaa
      241 agcaaagaaa tatatggatg caggtgatct agttcccgat gaagtagtga ttggaattat
      301 tcgtgacaga cttaaggaat ccgactgtgt caatggatac attctcgatg gatttcctag
      361 aacaactgag caggctaagg cattggacca gctgcttcag tcaattggaa gcaagctgga
      421 tgcagttgta aatattgcag ttcctgacaa agaattagtt tctagactcc tggaaagagc
      481 aaaaatagaa ggaagagctg atgacaatga ggagacgatc aaaaatcgtc tttccaatta
      541 taatcaaaag acttttcctc ttttggatta ttatcgctcc acaggtctat tgaaagaaat
      601 tgacggagtc ggcaaaccag aggaaattac tgctttaatt aagaagggca tttaaaaaca
      661 tggcaaaaga agaagcaatc acagtagacg gtacagtaat ggaacctctg ccgaacgcta
      721 tgtttcgagt tgaactcgaa aatggacaca agattttggc tcatatttcc ggtaaaatga
      781 gaatgcatta tattaggatt ttgccaggtg ataaggtgac ggtagaactg tcgccttatg
      841 acctgaccaa aggcagaata acttacagaa agaagtagga tgattatgaa agtaagagca
      901 tcagttaaga aaatttgcag cagttgcaag gtgattcgca gaaacaacgt aatccgtgta
      961 atttgcacaa acccaaaaca caagcagagg caaaagtaaa tggcaaggtt cgcaggaatt
     1021 gacttaccta gagaaaagcg gatcgtagta ggtctgacat atatatacgg cattggacgt
     1081 tcttcttctg aaaagatttt gaaaaaagcc ggagtagacg aaagcgttcg agtaaaaagt
     1141 ctgacagaag ctcaggaaaa tgcaatccgt aaagtccttg aagaaggata tgcattggaa
     1201 ggagatttaa gatcagagac tcagctgaac atcaagagat tgatgtcgag ttacttt
//
LOCUS       DAOQGJ010001532         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89135_length_1256_cov_3.068276, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001532 DAOQGJ010000000
VERSION     DAOQGJ010001532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89135_length_1256_cov_3.068276"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1255
                     /locus_tag="PK683_19380"
     CDS             35..1255
                     /locus_tag="PK683_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNH10671.1"
                     /translation="MFKSSLLVSFSKAFASLSNLLFMIYAVNILSRAENGHLQFYLGY
                     VPLLLAVAEFGLPNAMVKYLSAESENSMDTGSLLSASLFLKLVSLFILTLIGAVMVYF
                     YDFDPVTVFIIVSGGLITSFISYFESMLVAYREYASLAVWNPAGNLMRLGILYFSNVS
                     YERSLNHLDVLSIYCLSPGVTLFLFFILFRKHGIRWTGSFKGFKIKIKELALFNLWAF
                     AASLFAIASDRLEIFFLKKYHTPETVAVYGTALQLFSGFLIVFATLNSLILPRLSKAY
                     GTEDFNKFLIKAVAFCIFCSFLLSPGYFLAEPILNFLFQNKYSDSIPVFKILYPNYLL
                     QMVFAPLGIALFAMGKPSLLAFLALIRLIFGVFFDVLLIPELGPSGAAFSFLLGQVVS
                     WLVLAGYFLAAFWK"
BASE COUNT          272 a          260 c          244 g          480 t
ORIGIN      
        1 tttttcttct ttagggaaaa tcttccgaaa ggaaatgttc aaatcatctc ttttggtgag
       61 tttttccaag gcttttgcat cattatccaa tcttctgttt atgatttatg cagtaaatat
      121 tctttcccgt gcagagaacg gacatcttca gttttacctc ggttatgttc ctctccttct
      181 ggccgttgct gaattcggac tgccgaatgc tatggtcaaa tatctctcag ctgaatctga
      241 gaacagtatg gacactggaa gtctgctttc tgcctccctc tttctcaagc tggtatctct
      301 gtttattttg acactgatcg gcgcggtaat ggtttatttt tatgattttg atccggtgac
      361 tgtctttatc attgtatcag gcggactgat tacttcattt atcagctatt ttgaaagtat
      421 gctcgttgca tacagagaat atgcttctct tgctgtctgg aatcctgcag ggaatctgat
      481 gcgtctcggt atattatatt tttctaatgt gtcctatgaa agatctttga atcatttgga
      541 tgttctgagc atttactgtc tgagtcccgg ggttactctt tttctttttt tcatcctttt
      601 ccgtaaacac gggatacgat ggacaggttc gttcaaagga tttaaaataa agatcaagga
      661 acttgcactt ttcaatctct gggctttcgc ggcttctctt tttgcaatag catccgacag
      721 gcttgagata tttttcctta aaaaatatca cacaccggaa acggtcgctg tgtatggtac
      781 tgcacttcag ctgttcagcg ggtttttaat tgtatttgca acactgaatt cactgattct
      841 tccgagactt tccaaggctt acggaacgga ggattttaat aaatttctca tcaaggcagt
      901 tgcgttctgc atattctgtt catttcttct cagtccaggt tatttccttg ctgaacctat
      961 tttgaatttt ttatttcaga ataaatactc tgactccatc cctgtattca aaatcctgta
     1021 cccgaattat cttctgcaaa tggtttttgc tcctctcgga attgcgcttt ttgcaatggg
     1081 aaaaccgagc ttacttgctt tccttgcact gataagactg atctttggtg tctttttcga
     1141 tgttcttctg attcctgaac ttggtccttc cggtgcggcc ttttcctttt tattgggaca
     1201 ggttgtatcc tggcttgtgc ttgccggata ttttcttgcg gctttctgga aataat
//
LOCUS       DAOQGJ010001533         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89138_length_1256_cov_3.049958, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001533 DAOQGJ010000000
VERSION     DAOQGJ010001533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89138_length_1256_cov_3.049958"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PK683_19385"
     CDS             <1..665
                     /locus_tag="PK683_19385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH10672.1"
                     /translation="QALKILGKIDAVINNAGVGVLGLQETFTPEDFQRLFEINVFGVQ
                     RVNRAVIPHFRANGNGLLVHISSLLGRITVPFYGPYNASKWALEAMAENYRTELSGFG
                     IESCIVEPGGYPTSFIENLIRPSDQKRIQELGEFAKFPEEFLKGFEQGLAGNPEQNPR
                     KVATAILDLLKKPKGERPFRTIVDSMGMGTHIEDYNSHLAKVTEGIYSAFGIAHLLKV
                     KA"
     gene            716..>1256
                     /locus_tag="PK683_19390"
     CDS             716..>1256
                     /locus_tag="PK683_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH10673.1"
                     /translation="MPKTGMTCTEIREKAIEYAEERIRRLGFEKIRLIDVAKDLGISH
                     AALYQHFPDKAALLESVTEKWLMAADSELETVIKQKKTAAELIVLWFLSLHRIKRKKV
                     SLDPELFKAFDTAAEENKGCIQKHLSNAQRQLSDLVSQAMKEGSIKKDSVPKTVEILF
                     LGTIAFHYPKLVALNLQDSQ"
BASE COUNT          411 a          247 c          297 g          301 t
ORIGIN      
        1 ctcaggctct gaaaattctg ggaaaaatag atgcagtcat caataatgca ggtgtaggag
       61 ttttgggact tcaggaaacg tttacccctg aagattttca aagacttttt gagatcaatg
      121 tattcggagt tcagagagtc aacagagcag tcattccgca ttttagagcg aatggaaacg
      181 gccttctcgt tcacatatcc agtctgctgg gaagaatcac agtaccgttt tacggtcctt
      241 acaacgcaag caaatgggca ctggaagcaa tggcagaaaa ttacagaaca gagctttccg
      301 gtttcggaat tgaatcctgc attgtggagc ctggaggata tccgaccagc tttattgaaa
      361 atctcatcag acccagcgat caaaaaagaa ttcaggaact gggggaattt gcgaaatttc
      421 cggaagagtt tctcaaagga tttgaacagg gacttgccgg taatccggaa cagaatcccc
      481 gcaaggtggc aactgcaata ctggacctgc tgaaaaaacc gaaaggagag agacccttca
      541 gaacaattgt tgacagtatg ggaatgggaa cccatattga agattataat tcacacctcg
      601 caaaggtgac tgaaggaatt tattcagcat ttggaatagc acatttactc aaagtcaagg
      661 catgaaagat aaaacttgct tgcagaatgc aggcaagtaa gttcctgtaa gctgtatgcc
      721 aaaaactggg atgacatgta cggaaatcag agaaaaggca attgagtatg ccgaagaacg
      781 aatccgcagg ctcggatttg agaaaattcg gctgattgat gttgccaagg atctgggcat
      841 ttcccatgca gccttatacc agcattttcc ggacaaggca gctcttctgg aatcggttac
      901 cgagaaatgg ctgatggcgg ctgattctga attggaaacg gtaataaaac aaaaaaagac
      961 tgcagctgaa ctgatagtgc tctggtttct ctccctgcat cgaataaaaa gaaaaaaagt
     1021 atctcttgat ccggaattat tcaaagcgtt tgatacagcg gcagaggaaa acaagggatg
     1081 tattcaaaag catctgtcga atgcccaaag acagctgtca gacttagtct cacaggcaat
     1141 gaaggaagga agcataaaaa aagactccgt tcccaaaaca gtggaaattc tttttctggg
     1201 tacaatcgca tttcattatc ctaaactcgt tgctttgaat ctgcaagaca gccagg
//
LOCUS       DAOQGJ010001534         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89357_length_1254_cov_3.623019, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001534 DAOQGJ010000000
VERSION     DAOQGJ010001534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89357_length_1254_cov_3.623019"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PK683_19395"
     CDS             <1..558
                     /locus_tag="PK683_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH10674.1"
                     /translation="GKGHEIFLGVRDVNSFKGKELLSNPNTSVLPIKEAVKNSEVILI
                     SVPAPAAAETAESLGDTSGKIIIDSMNIVMGRGPAGFTNTADAILKNTQSREVIKCFN
                     CTGFNNMENTVYGTTAIDMFMAGDSENAKKTVRQLALDAGFAECYSIGGNDKFALLES
                     YAFFWINLAMMQGQGRNIGFKLLKR"
     gene            562..>1254
                     /locus_tag="PK683_19400"
     CDS             562..>1254
                     /locus_tag="PK683_19400"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNH10675.1"
                     /translation="MVLSRNFHPLRVLSYVRTEVVFSVLTVSAVYLIHKFKLADVSFS
                     FSISAILGSALAIFTAFRNNTAYSRWWEARTVWGNIINSSRIFARVLLTCTEAADRTK
                     ISSERTDSFLKESVFRQIAFAHSLRSALRRLPNDLSYKNFLSKSEAEDAEKSINVPNR
                     ILLLQSIYLKRAVSEGLIGPFDMISFENILSGLNNLQGPVKESKIHLFSDSMMSLLRI
                     KKICTNLIRGMKF"
BASE COUNT          374 a          257 c          272 g          351 t
ORIGIN      
        1 ggaaagggac acgaaatctt tcttggagtg agagatgtga acagtttcaa gggaaaggaa
       61 cttttatcca atccgaacac atccgtgctt ccgataaaag aagcggtaaa aaattctgaa
      121 gtcatactca tttccgttcc tgcacctgca gccgctgaaa cagcagaatc tctcggagac
      181 acttctggaa agatcatcat tgattcaatg aatatcgtga tgggaagagg tcctgccggt
      241 tttacgaaca ctgcagatgc gattctgaaa aacactcaga gccgggaagt cattaaatgc
      301 ttcaattgta caggtttcaa taatatggaa aacacagtat atggaaccac agcaattgat
      361 atgtttatgg ctggagacag cgagaatgca aagaagacag tcagacagct ggcactcgat
      421 gcgggatttg cagaatgcta cagcattgga ggaaacgaca agtttgccct actggaatcc
      481 tacgcattct tctggatcaa tctggccatg atgcagggac agggccgcaa tatcggattc
      541 aaacttttga aaagataact tatggttctt tcgagaaatt ttcatcctct cagggttctc
      601 agttatgtcc gcacagaagt tgtattttct gtgctgactg tttctgcagt ctacctgatt
      661 cataagttca aacttgcgga tgtttccttt tccttttcca tttctgcgat tttgggaagt
      721 gcacttgcga tattcactgc cttccgaaac aatacagctt attccagatg gtgggaagca
      781 aggactgtat ggggtaatat tatcaacagt tcaaggattt ttgccagagt tctcctcaca
      841 tgcactgagg cagcagacag aacaaaaatt tcctctgaac gtacagactc ctttctgaaa
      901 gaatcggtct tccgtcaaat cgcttttgct cattctctca gatctgcact tcgcagacta
      961 ccgaacgatc tttcttataa gaattttctt tcgaaatcgg aagcggagga tgcagaaaaa
     1021 agcatcaatg tcccaaacag aatcctgctt cttcaaagta tttatctcaa aagagcagtc
     1081 agtgaaggtc tgatcggtcc cttcgatatg atcagttttg agaatattct atcgggcctg
     1141 aacaatctgc aggggcctgt gaaagaatca aaaatacacc tcttctcaga cagtatgatg
     1201 agtctgttgc gaattaagaa aatttgtaca aatttaataa ggggtatgaa gttt
//
LOCUS       DAOQGJ010001535         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89498_length_1253_cov_3.069282, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001535 DAOQGJ010000000
VERSION     DAOQGJ010001535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89498_length_1253_cov_3.069282"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..>1253
                     /locus_tag="PK683_19405"
     CDS             10..>1253
                     /locus_tag="PK683_19405"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10676.1"
                     /translation="MNQKAPVVPSIVSAYNIYALDFHFYSINGYFPISEKHTMSYLRF
                     YLNKKDLDEKKDSIFLFNPVLKYLKEKYPQMTTISSGADNGVSMYFAYQLKPDLMIQL
                     GILYYLRVEDPNEPGVFRYQYCADAYAQYSDQRILALNDVSYACSEGTREEAEKITEK
                     LWELREETPIIDQGQVVSQIGIICKTVEGVYYIADKEFTEHSAGFKDFDLDMHYGEGF
                     TEKFHRLLVERLKTRSNGIVLFHGSPGTGKTFYIRRVIRELYNLPDKKIIIVPNTFVK
                     DIATPEFFNFLYSYIQDNFVSLVLVIEDAENILKKRSSRNSFEAISNILNMTDGLLND
                     FVHIQIVATFNTELENIDTAILRHKRLLARKEFQRLGLDAAKKLAKSVDTPAELIAKM
                     TKETYSLAEIFSLNEQEADSLL"
BASE COUNT          391 a          227 c          267 g          368 t
ORIGIN      
        1 acatacagaa tgaatcaaaa agccccggta gtaccgtcaa tagtaagtgc atacaatatt
       61 tatgcactcg attttcattt ttattccatt aacggttatt tcccaatttc ggaaaagcat
      121 acgatgtctt atcttcgttt ttatctgaat aagaaagatt tggatgaaaa aaaagattct
      181 atttttcttt ttaatcctgt cctgaagtat ctcaaggaaa aatatcccca aatgactaca
      241 atctcttcgg gcgcagataa tggcgtgagt atgtattttg cgtatcagct gaagcctgat
      301 ctgatgattc agcttgggat tctttactat ctcagagtgg aagatccgaa tgagcctggg
      361 gttttccgtt atcagtactg tgctgatgct tatgctcagt attcagatca gagaattctg
      421 gctttaaacg atgtatctta tgcctgctcg gaaggaacta gagaagaagc agaaaagatt
      481 actgaaaagc tctgggaact ccgtgaggaa actccgatca ttgatcaggg tcaggtagtc
      541 agtcagatag gcattatctg caagacagta gaaggtgttt attatatagc agataaagaa
      601 tttaccgaac acagtgccgg attcaaggat tttgatctgg atatgcatta cggagaagga
      661 tttactgaaa agtttcacag acttctggtg gaaagactca agactagaag caacggtatt
      721 gttctgtttc acggttctcc gggtacagga aagacttttt acatcagaag ggtcatcaga
      781 gaactctaca atcttccgga taaaaagatc atcattgttc cgaatacctt tgtgaaagac
      841 attgcgactc cggaattctt caatttcctg tattcctata ttcaggacaa ttttgtaagt
      901 cttgttcttg tgattgaaga tgcagaaaat attctgaaaa aaagaagcag cagaaacagc
      961 ttcgaggcaa tttccaatat cctgaatatg actgacggtc ttctgaatga ttttgttcat
     1021 atccagattg tggctacatt caacactgag ctggaaaata ttgacactgc gatcctcagg
     1081 cataagaggc ttctggcaag aaaagagttt cagagactgg gattggatgc cgcaaagaaa
     1141 ctggcgaagt cagtcgatac tcctgcggaa ctgattgcaa aaatgaccaa agaaacttat
     1201 tcattggcag aaatcttttc tttgaacgag caggaagcag acagccttct ttt
//
LOCUS       DAOQGJ010001536         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89619_length_1252_cov_3.233083, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001536 DAOQGJ010000000
VERSION     DAOQGJ010001536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89619_length_1252_cov_3.233083"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PK683_19410"
     CDS             complement(<1..209)
                     /locus_tag="PK683_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10677.1"
                     /translation="MKKIIFMALILFSGALFSEAKKEENSPKKEDSGNAAKEEKGKFY
                     ITAKRGLNIRSEPSKTAVVIVKMPYG"
     gene            complement(805..1252)
                     /locus_tag="PK683_19415"
                     /pseudo
     CDS             complement(805..1252)
                     /locus_tag="PK683_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT          376 a          263 c          247 g          366 t
ORIGIN      
        1 ccgtaaggca ttttcactat cacaactgca gtcttggaag gttccgatct gatgttcagc
       61 cctctttttg cagtgatata aaactttcct ttttcttcct tcgcagcatt gccggaatct
      121 tctttcttgg gactgttttc ttctttcttt gcttcagaaa aaagcgcacc tgaaaaaaga
      181 atcaaagcca taaaaataat tttcttcaat ctgtttctcc tgagtaccgg aataatccgt
      241 aaagacatgc agtcaatgaa tttactgaat tagaattctg cagtttacag gcaaatgaag
      301 ctgaacctaa aatccgctta gacttttttt atcgtcgaag tgtttaaaaa agtttccggt
      361 atactgattt tttagagcat ttgtgcaatt gtattttccg atttccagcc aggcttttgt
      421 tttgtctgca gactgttaaa agtcctcaag agtttgaaac ctgggcagta cagtcccaaa
      481 gggattccgt aaaaaatgaa aatgccgaga gcgaatcagt cagccctgac tccggttaat
      541 ggtatatttt ctcagtattt ttactgaaat tgtatctttg actcagccca gtgaaatttg
      601 aaaaaaactt cagcaaaaaa ctgctcctct tttcagacag gaagtcatta tttaaacttt
      661 ctgtctgatt cgcagtaaca gttcatctca gttgattaca tcgagtgcat caaacttttt
      721 ctttttctta tgaccctttt tgaagatcct ttcggcaatc cagatctgca ttttttctat
      781 ttttctcttg gtctctcgag caaacttgta aagaacaggg acaagcatca gagtgagaag
      841 agtggcaaac gcaagtcccc agcctattgc aagagccatc ggcatcagaa acggatcaga
      901 tccgccgatt ccgtacgcag taggaagaat tccaaggact gtagtgattg tagtcagcat
      961 gacaggacga agacgcacaa gaccagtctg caataaaaga tcatgactgt ttatcccagg
     1021 attctccttt ttcagctgat tggcaaagtc cacaagaacg attgagtcat tcaccacgat
     1081 tccggaaaga ccgataatcc cgataatgct aaggaagctg aaatagtgtc catgggtcag
     1141 aaatgccact gccacaccga tgaaggaaaa cgggactgcc gaaagaacga taaagggctg
     1201 aacaatggat ctgaaaagag atgccagaat cataaagatg actatgatcg cg
//
LOCUS       DAOQGJ010001537         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89621_length_1252_cov_3.201337, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001537 DAOQGJ010000000
VERSION     DAOQGJ010001537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89621_length_1252_cov_3.201337"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PK683_19420"
     CDS             <1..494
                     /locus_tag="PK683_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10678.1"
                     /translation="KLTTNETIPCERIPVPSMEDIWALSVDGGWSHHPEKISTNNVCK
                     GDLQEYFYKQYSNNESYVAEKHVPRTRRSSCTNNVLFHGKDKLYSLMIEKTAKEEVPS
                     ASYDAESVKKDVVTYNTEGRKRSFFYECRPTDPQKRWDTCTCVLYATYRNGKSGLSER
                     IKK"
     gene            491..>1252
                     /locus_tag="PK683_19425"
     CDS             491..>1252
                     /locus_tag="PK683_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10679.1"
                     /translation="MKFLIYFLILFSSIELTADSIPLREGWEVLDTDLPVEEISADKI
                     SFLEGWKKYSTNIPLTAEKTHLLKLRLPEKEFRQPVLHFGKNIFSFEVIYAGKSVYKF
                     ADKTQFEPGRFLGWVSQIVSIPKNREDNTVYLRVYSGAKIGFAAVSYGEKDEIYEEII
                     SSNLPGIAVFVISMLLGIIFFSIYIFSSRDLFYLATASFYIFIGIWLFNINPISQFVL
                     PESPWRLRSEYISLYLAPIGCLFFLESIVISRLNII"
BASE COUNT          396 a          257 c          251 g          348 t
ORIGIN      
        1 ctaaacttac tacaaacgaa acgattccct gtgaacggat acccgtcccg agtatggaag
       61 acatctgggc tttatcggtt gatggaggct ggtcgcatca ccctgaaaag atcagcacaa
      121 ataatgtctg caaaggagac cttcaggaat atttctacaa gcagtattca aacaatgaaa
      181 gctatgttgc cgaaaaacat gtacccagaa caagaaggag cagctgcaca aacaatgttc
      241 tgtttcatgg aaaggacaaa ctgtacagtc tgatgataga aaaaacagca aaggaagaag
      301 ttccgtcggc ttcctatgat gctgaatccg taaaaaagga tgtggtgact tacaatacgg
      361 aaggaagaaa aagaagcttc ttttatgaat gcagaccgac tgatccgcag aaaaggtggg
      421 atacctgcac atgcgtgctg tatgcgactt acagaaacgg aaagtcagga ctttcagaaa
      481 gaattaaaaa atgaaatttc ttatttattt cttaattctt ttttcatcta ttgaactgac
      541 tgcagacagt attccccttc gggaaggctg ggaagtgctc gatacagacc ttcctgtaga
      601 agaaatatct gcagacaaga tttcatttct ggaaggatgg aaaaaataca gcacaaacat
      661 ccctttaact gcagagaaga ctcatctcct taaactcaga cttcccgaaa aggaattcag
      721 acagcctgtt cttcattttg gaaaaaatat tttttccttt gaggtcatct acgctggaaa
      781 atcagtttat aagtttgctg ataaaactca gtttgaaccg ggaagattcc tcggctgggt
      841 atcccagatt gtatccatcc ctaaaaacag ggaagacaat accgtttatt taagagtcta
      901 cagcggagcg aaaatcggat ttgcagcggt cagctacggt gaaaaggatg aaatttatga
      961 ggaaatcatt tcctcgaatc ttcccggtat tgctgtattt gtcatcagta tgcttctcgg
     1021 aatcattttt ttttccatat acatattcag cagcagagat cttttctatc tggcaactgc
     1081 atcattttac atattcatag gcatctggct cttcaatatc aatccgattt cccagtttgt
     1141 tctgcccgaa tcaccctgga gactcagatc agaatacatc tctctgtacc ttgcaccgat
     1201 cggatgtctt ttctttctgg aatccatagt aatctcgcga ctgaatataa ta
//
LOCUS       DAOQGJ010001538         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89639_length_1252_cov_2.812865, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001538 DAOQGJ010000000
VERSION     DAOQGJ010001538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89639_length_1252_cov_2.812865"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PK683_19430"
     CDS             <1..633
                     /locus_tag="PK683_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNH10680.1"
                     /translation="IINLAAGFISRTLPEYRIHLYQTLAKFAFEKGHDYWGFRFMGWA
                     MHYLGDLTQPYHSTVFPGKSAGGMLWINMKSAAGFKSSYEQAVERLGDRHTAIEHYQY
                     SAMEKAIMKYPSSTLLSAYQILENDSKFPAYDDSYPRAVIAKESNDKADMLDELIDNS
                     AEIMKFKSPDYDPPDFKEDDSTKAINKFLHGLFTSFGSHIRNFAKSVMKK"
     gene            843..>1252
                     /locus_tag="PK683_19435"
     CDS             843..>1252
                     /locus_tag="PK683_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10681.1"
                     /translation="MKLFCVFILFSVSLKSESKEDKVELIISDPSKKAEGSWGKEADQ
                     VEAIQDIKIIDELSEESTIARMQEARNHFNTSLATFKASEKQIKDKREAAAKETNPQD
                     KYAWQKKSREMELDRECKRISQEGRKNAVLELVKG"
BASE COUNT          393 a          242 c          278 g          339 t
ORIGIN      
        1 atcatcaatc ttgctgctgg attcatcagc aggacgctcc ccgaatacag gattcatctt
       61 tatcagacat tggcaaaatt tgccttcgaa aagggtcatg actactgggg cttcagattt
      121 atgggatggg ctatgcatta tctgggggat ttgactcagc cctaccattc cacagttttc
      181 cctggaaagt cagcaggcgg gatgctctgg ataaatatga aatcagcggc aggtttcaaa
      241 tcctcttatg agcaggcagt ggaaagactt ggggacagac atacagccat tgaacactat
      301 cagtattctg ctatggaaaa ggcaattatg aaatatccct catcgactct tttaagtgca
      361 taccagattc tggaaaatga cagcaaattt ccagcgtatg atgattctta tccaagagct
      421 gtcattgcaa aagagtcaaa tgacaaggca gatatgcttg atgaactgat tgacaacagt
      481 gctgaaatta tgaaattcaa gtctcctgac tacgatcctc cggatttcaa ggaagatgat
      541 tctacaaagg caatcaacaa gttcctgcat ggactgttta cttcctttgg atcgcatatc
      601 cgaaatttcg caaaatcagt gatgaaaaaa taggcattcc tgttctgtct caacggaaag
      661 agccgagttt gtctttctga aaatggaagc atcctgtgaa ttccctgcga aattgctgga
      721 tgcttttttt attgcaggca agtcctgctt tgatctgaca gatctcgatt ccggcaatac
      781 atccctaaag tctcattaag attccttctg tttttaccga taaattattc atgaatgcat
      841 ttttgaaatt attctgtgtt tttattctgt tttccgtttc gctgaaatcg gaatcgaaag
      901 aagataaagt tgaacttatc atatctgatc cgagcaagaa ggcagagggt tcctggggga
      961 aggaagcaga tcaggtggaa gccattcagg atatcaaaat cattgatgaa ctgagtgaag
     1021 aaagtacaat tgccagaatg caggaagcca gaaatcattt taacacttct cttgccactt
     1081 ttaaagcgtc tgaaaaacag atcaaagaca aaagagaagc ggctgcaaag gaaacgaatc
     1141 ctcaggacaa gtatgcctgg cagaaaaagt ccagggagat ggagctggac agggaatgca
     1201 aaagaatttc ccaggaaggc agaaaaaatg ctgttctgga acttgtaaaa gg
//
LOCUS       DAOQGJ010001539         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89665_length_1252_cov_2.374269, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001539 DAOQGJ010000000
VERSION     DAOQGJ010001539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89665_length_1252_cov_2.374269"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1168
                     /locus_tag="PK683_19440"
     CDS             104..1168
                     /locus_tag="PK683_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10682.1"
                     /translation="MNSLFGIAIPRSLFNLIGKVYKKYYELSFFKKTLFGYFFASLLF
                     FYFHEDHLPLGIIFLLVVPVSLGSLILAALELQILAVVIRNEERVWPEILFLLFSLIT
                     VFFTGLLENRAGWVFLFFASSIYIFSVTLKQKLLRQILLLGLSLFICGIISFKGLQFM
                     EFYVFKYNHQKKYNSVEIDLTAWKSDDSNRIFTHSEIPLKLELPEGFFFHNPKNLSSK
                     DETGTGQIAGIISPSEKDTTPYPNIRIFLVSSLVNLSDENLRNEYQEFLDFQVKQGEI
                     ESLSFEKEFVYEKQKWKGQFWKFYDILRPRDARSGYYLIQLNSGNKLILDVRENLTQD
                     KEFHGKEIEQILEKIQLSSD"
BASE COUNT          359 a          240 c          247 g          406 t
ORIGIN      
        1 atattcaaat agctttccaa gaaaatcagt tatttttgaa tagtttctca ggaatgattt
       61 tatggacttt tccaaggtct ggtaatcact cttcaagata ttgatgaact ctttattcgg
      121 catagctatc ccccgctctc tatttaactt aatcggcaag gtttacaaaa agtattatga
      181 actaagtttt ttcaaaaaaa ctctttttgg atattttttt gcgagtctgc tgttttttta
      241 ctttcatgag gatcatctgc ctcttggaat catatttctt ctggttgttc ccgtttcttt
      301 gggttctctg atccttgcag cactggaatt gcagattttg gccgtggtca taaggaatga
      361 ggagagggtc tggccggaga tccttttcct tctgttcagt ctgattactg tcttttttac
      421 aggccttctg gaaaacaggg ccggatgggt tttccttttt ttcgcttcct caatctatat
      481 tttttccgtt accctcaaac agaagcttct gaggcagatt ctgctcctcg ggctttcgct
      541 ctttatctgc ggaattattt cattcaaagg acttcagttt atggagttct atgtcttcaa
      601 atacaatcat cagaaaaaat acaactcagt ggaaattgac ttaactgcct ggaaatcaga
      661 cgattcaaat cgaattttca ctcattcaga aatcccatta aaactggagc ttcctgaagg
      721 ttttttcttt cataatccca aaaatctgtc ctccaaggat gaaacgggaa caggtcagat
      781 tgcagggatc atttctccca gtgaaaaaga cactacacct tacccgaata tcagaatttt
      841 tcttgtttcc tcattggtaa acctgagcga cgaaaactta aggaatgaat atcaggaatt
      901 tctggatttt caggtcaagc agggagaaat tgagtcattg agctttgaga aggaatttgt
      961 ctatgagaaa cagaaatgga aaggacagtt ctggaaattt tacgacatcc tgagaccaag
     1021 agatgcacgg tcaggatact atcttattca attgaacagc ggaaataaac tgattttgga
     1081 cgtcagagaa aatctcactc aggataagga atttcacgga aaggaaattg agcagattct
     1141 ggaaaagatt caactgagtt cggactagga actctccata atcttcagta tccagccctt
     1201 cagttcgggc gagttcatta cggaaaaatg gttgtatcct gaaagaatca gg
//
LOCUS       DAOQGJ010001540         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89708_length_1252_cov_1.649123, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001540 DAOQGJ010000000
VERSION     DAOQGJ010001540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89708_length_1252_cov_1.649123"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PK683_19445"
     CDS             complement(<1..464)
                     /locus_tag="PK683_19445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH10683.1"
                     /translation="MIELKRVSKSFHSGSSELRILDSLSAEIGQGEFAAVMGPSGSGK
                     STFLGIAAGLDSPDSGEVIIGGTLITGKSESELSRFRSENIGFLFQNFLLIRNMTVIE
                     NVSMPLAVSGRYSRKEIAERAENALRKVSMQDRMTHFPSQLSGGQQQRVAIAR"
     gene            complement(457..1170)
                     /locus_tag="PK683_19450"
     CDS             complement(457..1170)
                     /locus_tag="PK683_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HNH10684.1"
                     /translation="MKNNDFISEADRKTGIHRIIEMSSICAFLVMEFLLFKRVWESRI
                     HFNSDIFGSSLSVLLTVILSYIAADFISGYVHFLGDSFGDENTPILGQSFIRPFREHH
                     TDPKGITRHDFIETNGNNCIVSLPAQAAVYFLQVPTSAVWNLISLFTAFLLLWVFFTN
                     QFHKWAHEDFPPRGAVFLQKAGIILSHEHHSVHHRSPYDRFYCITSGWLNPLLDRMNF
                     FSWNRKIINKSFNKDKMHD"
BASE COUNT          361 a          285 c          257 g          349 t
ORIGIN      
        1 cgggcaatgg cgacccgttg ttgctgaccg cctgaaagct gtgaaggaaa atgagtcatt
       61 ctgtcctgca tcgaaacctt ccttagtgca ttttccgccc tctccgcaat ttcctttctg
      121 gagtatctcc cgcttacagc caggggcata gatacatttt caatcaccgt catattcctg
      181 atcagaagaa agttctgaaa gagaaaacct atgttttcag aacggaatct ggacagttcc
      241 gattcagact ttcccgtaat taatgttccc ccgataatga cttcccctga atcaggagaa
      301 tcgagtcctg ccgcgattcc cagaaacgtg gattttcctg acccggaggg tcccatcact
      361 gcagcaaact ccccctgtcc gatttctgca gagagcgaat ccagaattct gagttctgaa
      421 ctgcctgaat gaaaactctt tgagaccctt ttaagttcaa tcatgcattt tatccttatt
      481 aaatgattta ttaatgattt ttctgttcca gctgaaaaaa ttcattctgt ccagcagagg
      541 gttcagccat ccggacgtaa tgcagtagaa gcggtcataa ggacttctgt gatgaacaga
      601 atgatgttca tgtgaaagta taatccctgc tttttgcaga aaaacggctc cgcgtggggg
      661 aaagtcttca tgagcccatt tatgaaactg gtttgtaaag aacacccaaa gcagaaggaa
      721 tgcagtgaag agagaaataa ggttccatac agccgaagtc ggcacctgca gaaaataaac
      781 agctgcctgt gccggaaggg atacaatgca gttgttcccg tttgtttcaa tgaaatcatg
      841 ccttgttatc cctttcggat cggtatgatg ttcccggaaa ggacggatga aagactgccc
      901 cagaatcggt gtgttctcat ccccgaaact gtctcccaga aaatgcacat aacccgaaat
      961 aaaatcagcc gcaatatatg acaatatgac tgtgagcaaa acagacagag aagatccgaa
     1021 tatgtcagaa ttaaagtgaa tccggctttc ccatactctc ttgaagagaa gaaattccat
     1081 tacaaggaat gcacagatgg aagacatctc gatgatcctg tgaatgcctg ttttcctgtc
     1141 tgcttcggat ataaaatcat tatttttcat aagaatctca gctcctgttt tttatgttct
     1201 cagtcagaat atcataaaac tcttttgcgt cctgaaattt tttcttcatt ac
//
LOCUS       DAOQGJ010001541         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89778_length_1251_cov_2.944816, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001541 DAOQGJ010000000
VERSION     DAOQGJ010001541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89778_length_1251_cov_2.944816"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK683_19455"
     CDS             complement(<1..546)
                     /locus_tag="PK683_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10685.1"
                     /translation="MLKYRIMKPSSLIFPMVILLFFSLSPVFAEGLNEKEYSDIFHYY
                     GRPAEKFSIVYDLNSSIESRIKSIPSDILEKYREEDRQLLNDKINYNSYKVQASEMQS
                     LSSFLKLLPESYTEILKKKLIGIFFVEGFLGGGLTDWLLKDDENEMYYIILLNSDILK
                     KSFSEWETEKYSSAFMKSAGDD"
     gene            complement(912..>1251)
                     /locus_tag="PK683_19460"
     CDS             complement(912..>1251)
                     /locus_tag="PK683_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10686.1"
                     /translation="EALNPFFVSFLAIISGLLAEQAYEKIYHTGQSFFKEKDVKENRR
                     WAVRLNAEIQKQNKSLAELQRYTGAPLELLQKWSEEKESVPEKEQKIIAAWLGISGRE
                     LFTDEAPEIK"
BASE COUNT          362 a          285 c          226 g          378 t
ORIGIN      
        1 gtcatcaccg gcagatttca taaaagcaga actgtatttt tcggtctccc actctgaaaa
       61 actcttcttt aaaatatcag aattgagaag gatgatatag tacatttcat tctcatcatc
      121 cttcagaagc cagtccgtga gtccccctcc taaaaagcct tccacaaaaa aaattcctat
      181 cagctttttc ttcagtattt cagtgtatga ttcaggcaga agcttcagaa aactggaaag
      241 actctgcatt tcagaggctt gaaccttata ggaattgtaa ttgattttat cattcagaag
      301 ctgcctgtcc tcttcccggt atttttcaag tatatcagaa ggtatgctct ttatcctgct
      361 ctcaatggaa gaattcagat catatactat actgaatttt tccgcaggtc tgccgtagta
      421 gtgaaaaatg tccgaatact ctttttcatt caagccctct gcaaatacag gacttaatga
      481 aaagaataac aatataacca tggggaatat gagagaactg ggtttcatga ttctgtactt
      541 tagcatactc ttatttctgt ttactccgca aaactcattg tgaaaaagat actgagtaac
      601 tcacctgcgg cgcctgccag cggattgagg gctcgcaaac ttgaaagctg ctgcaaactt
      661 gaaagccgct gcaaacccgg aagctggcta aactcccgca agcctgaaag atgctgttct
      721 tacagattta ttagattcat ctaattcttc gtaaaaactg caagttctct gctttatatt
      781 ctttttgcgc aaggtgagtg agtaaaagtc agtcaccggc gaagaagcat ttactcttcg
      841 attcagccca gctcaaaatg acttttacac agcctcactg tcttcaaata ctgagaagaa
      901 caaacccgca gtcactttat ttcaggggct tcatctgtaa aaagctctct gcctgaaata
      961 ccaagccatg ctgcaatgat cttctgttct ttttcaggaa ctgattcttt ttcttccgac
     1021 cacttctgca gaagttcaag aggagcacct gtatatcttt gaagttcagc aagggattta
     1081 ttctgcttct gaatctctgc attcagacga acagcccagc gtctgttttc cttgacatcc
     1141 ttttccttga agaaggactg tcccgtatga tagatttttt cataagcctg ttctgccaga
     1201 agacctgata taattgccag aaacgacacg aaaaacggat tcagcgcctc a
//
LOCUS       DAOQGJ010001542         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89850_length_1251_cov_1.633779, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001542 DAOQGJ010000000
VERSION     DAOQGJ010001542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89850_length_1251_cov_1.633779"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..409)
                     /locus_tag="PK683_19465"
     CDS             complement(218..409)
                     /locus_tag="PK683_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10687.1"
                     /translation="MSDSEKMPVSERIKILIADENRRKSAIYWTLAAAALLFGIFFVY
                     KKISKKSPVFNFPKEHKKP"
     gene            complement(406..>1251)
                     /locus_tag="PK683_19470"
     CDS             complement(406..>1251)
                     /locus_tag="PK683_19470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH10688.1"
                     /translation="YLSELVNFQKDFYDNYNDLLHLTKFRADKGDISQLEYDRIFLEK
                     LRIEKEVQDAEIQRVQVAKEIRFFAGIPGTASRKLKLKGNLRFFTTAELGVDLKNYDM
                     ENRPDLKAHKLRVRQSRLNVDLKKKEGTHFGPFANVGAEIRHKGNETYTGLFLTVPLK
                     VFDRNQGEILKAEESYKKTSLELESRRKLIEIEIRTAVRELEAREELLRTYTKNRLLE
                     KNKEVQEKSRTAYVKGASNLVTFLEAEKNYINLLKGYYEQLYLYYNAIEQFRAAVGRL
                     QENGK"
BASE COUNT          318 a          275 c          196 g          462 t
ORIGIN      
        1 ttttggatct aaaatcttta gacttttctg aacaaatgtt atattatcct ggtccaggaa
       61 tatcatttta ctaatattat gattattatg attcactact agatagattt cgttgaaacg
      121 tatctcttgt ttttggaatt ttacaaactt tcttaatttt ttatttcttt acaattgaag
      181 tttgttctag catagcattt tgagtattta tagctttcta tggcttttta tgctcctttg
      241 ggaaattaaa cactggcgat ttctttgata tttttttata aacaaaaaat attccaaata
      301 aaagtgcagc cgctgcaagc gtccagtaaa tggcagactt tcttctgttc tcatctgcaa
      361 tgaggatctt tattctttca gaaaccggca ttttctccga atcactcatt tcccgttctc
      421 ctgcagtctt ccgacagccg ccctaaactg ctcaatagca ttataatata aatataactg
      481 ttcatagtat cctttcagga gattgatata gtttttttcc gcttccagaa aagtgacaag
      541 attggatgca ccttttacat atgcagttct ggatttctcc tgaacttcct tgttcttttc
      601 gagaagccgg tttttggtat aggtcctaag cagctcttct cttgcttcaa gttctcttac
      661 cgcagttctt atttcaattt caatcagctt tcttctcgat tccagttcga gagatgtctt
      721 cttgtaggat tcctctgctt tcagaatctc cccctggttt ctgtcaaaaa ccttgagagg
      781 cactgtaaga aaaagtccag tgtacgtttc atttcccttg tgcctgatct ctgctcccac
      841 attcgcaaaa ggtccgaaat gagttccttc cttcttcttc agatccacat tcagtctgct
      901 ctgtcttact ctgagtttat gcgctttcag atcaggcctg ttttccatat catagttttt
      961 caaatccaca ccgagttctg cagtagtgaa gaatctcaga tttcccttca gcttaagctt
     1021 tctgcttgca gtgcctggaa ttccggcaaa aaagcggatt tcctttgcca cctgcactct
     1081 ctgaatctct gcatcctgca cctctttttc tattctcagc ttttccagaa aaatcctgtc
     1141 atactccagc tgagagatat cacctttgtc agcacggaat ttagtcagat gaagaagatc
     1201 attataattg tcataaaaat ctttctgaaa attcacaagt tcagaaagat a
//
LOCUS       DAOQGJ010001543         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_89930_length_1250_cov_2.441004, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001543 DAOQGJ010000000
VERSION     DAOQGJ010001543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89930_length_1250_cov_2.441004"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..226)
                     /locus_tag="PK683_19475"
     CDS             complement(2..226)
                     /locus_tag="PK683_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10689.1"
                     /translation="MRLFGTTNLVISNDKNLGVFMFDFDNIDYFSVKEETKFLSECFD
                     IDIFILQSSKNSYHIIIDFSTIFQNFKQKY"
     gene            269..826
                     /locus_tag="PK683_19480"
     CDS             269..826
                     /locus_tag="PK683_19480"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HNH10690.1"
                     /translation="MNFCFCGGLSMHGNNTPVSVIMHFREKHLTSNTANLGLLTLSKF
                     RIYERGLKEAPFRFSSIPVTENELVLFLYPDEGAVLLDDEFSDKYKGPYHLVVPDGSW
                     GQASKIRKRERLEHLMSVRLPADVRGEYTLRKSTSFNRLSTFEAVAYAMRYLEGEEMS
                     LSLLSCFRLMNERVEQSRKNPGLLL"
     gene            complement(890..>1250)
                     /locus_tag="PK683_19485"
     CDS             complement(890..>1250)
                     /locus_tag="PK683_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002180133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH10691.1"
                     /translation="RIEDKSNLQIISITVNPEIDNPEMLRKFREMYKIKEESWVFLTG
                     SKNFIFDLARKQFGAEVKVLLGQNDLTDFVHTENVYLIDRKNYLRGIYRAKGTGDLER
                     LLVEMKTLRQEDFGKKL"
BASE COUNT          373 a          237 c          231 g          409 t
ORIGIN      
        1 attagtattt ttgtttaaaa ttttgaaata ttgttgaaaa atctataatt atatgataag
       61 aatttttgct actttgtaaa ataaaaatat ctatatcaaa acattctgat aaaaatttag
      121 tttcttcttt tacagaaaaa taatcaatat tatcaaaatc aaacataaaa actcctaaat
      181 ttttatcgtt agatataact aaattagttg taccaaataa tctcatattt ctacacctaa
      241 caaatataaa tttttatata tcataaacat aaatttctgt ttctgcggcg gactgagtat
      301 gcatggaaac aacacccctg tttctgtcat tatgcatttc cgtgaaaaac atctgacttc
      361 aaatacagca aatctcggtc ttctgactct ctcaaagttt cgtatatatg aaagaggatt
      421 gaaggaagct cctttccgtt tcagttccat ccctgttaca gagaatgagc ttgtgctttt
      481 tctgtatcct gatgaagggg ctgtactgct tgatgacgaa ttttctgata aatataaagg
      541 tccttatcat ctggttgtcc ctgacggatc ctggggacag gcgtccaaga tcagaaaacg
      601 ggaaaggctg gaacatctta tgtctgtccg actgccggct gatgtaagag gtgagtatac
      661 cctcagaaag agcacatctt tcaacaggct ctccactttt gaggcggttg cctatgcgat
      721 gaggtatctg gaaggagagg aaatgtctct cagtcttctt tcctgtttca ggctgatgaa
      781 tgagagggtg gagcagagca gaaaaaatcc tggactgctc ctctgattca gaaaggaaac
      841 gatagatcca ttactcagtc cgtggagcag cctctgataa aatggacgac tacagttttt
      901 ttccaaagtc ttcctgacga agcgttttca tttcgaccaa gagtctttcg aggtcgccag
      961 tgccttttgc tctgtaaata ccccgaaggt aatttttcct gtcaatcagg tatacatttt
     1021 cagtatgaac aaaatcagtc agatcattct gtccgagaag tactttcact tctgcgccaa
     1081 actgttttct ggccagatcg aagatgaagt tttttgatcc ggtgagaaat acccagcttt
     1141 cctccttgat tttatacatt tctctgaatt ttcgaagcat ttccggattg tcaatttccg
     1201 gattaacagt aatggatata atctgcagat ttgatttatc ttcaatgcgg
//
LOCUS       DAOQGJ010001544         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90011_length_1249_cov_3.325796, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001544 DAOQGJ010000000
VERSION     DAOQGJ010001544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90011_length_1249_cov_3.325796"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..901)
                     /locus_tag="PK683_19490"
     CDS             complement(101..901)
                     /locus_tag="PK683_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNH10692.1"
                     /translation="MLISSFSNEKVKHAVRLRDKKYREQSREILIEGYREIRRALISG
                     KIRFKSILISEDCFLGENEPELIEEILKQGARLLEFPRKVFEKISYRDRPDGLIALAE
                     EPDFSLGEKNIEGSIFLLIEGVEKPGNLGTILRTAEGAGTDAVFVSDSKLDIFNPNVI
                     RSSTGTVFTLPVYTGSLEEILGRMQEIGAASFAVTPEASSCYYNENLTGNTLFIFGSE
                     QYGLSPLAKKKADRRIFIPMKGEADSLNLAMSAGIILYESVRQRAMLR"
     gene            971..1219
                     /locus_tag="PK683_19495"
     CDS             971..1219
                     /locus_tag="PK683_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH10693.1"
                     /translation="MPTYEYKCSTCSTVFEHFQSIKDEPLKECLCEKKGSVSRLISSG
                     TGIIFKGSGFYVNDYKKSGSEKSSDSSSSSTSSASSGS"
BASE COUNT          326 a          290 c          255 g          378 t
ORIGIN      
        1 acaatctcag tttcatttcc tttttctgaa aaatgccgga tgatttcgag ggaccggctg
       61 atatggccga atccgtgatg actgatgtaa taagtgaatt tcatctaagc attgccctct
      121 gccgcactga ttcatacaga atgattcctg cagacattgc cagattcagt gaatccgcct
      181 cccctttcat cggaatgaaa attcttctgt ctgccttctt cttggcgagg ggactcagtc
      241 catactgttc gcttccgaat ataaaaagtg tgtttcctgt aagattttca ttataatagc
      301 atgatgaagc ctccggtgtg acagcaaaac ttgcggcacc gatttcttgc attctgccta
      361 gaatttcctc caggcttccc gtgtatacag gaagagtgaa cacagtgcct gtgctcgacc
      421 ggatgacatt cggattgaat atgtcaagtt tcgaatctga tacgaagacg gcgtctgtac
      481 ctgccccttc ggctgtccga agaatggttc ccaggttgcc cggcttttcc acgccttcaa
      541 tcagaagaaa tatgctccct tcaatgtttt tttctccaag cgaaaaatca ggttcctctg
      601 cgagggcaat cagcccgtca ggcctgtccc ggtaggaaat tttttcgaat acttttctcg
      661 gaaattccag aagtcttgca ccctgtttca ggatttcttc aatcagctcc ggctcatttt
      721 ctcccaaaaa acagtcttct gagatgagaa ttgacttgaa tctgattttt cctgaaatca
      781 gtgcccttct gatttctctg tagccttcga tcagtatttc ccgggactgt tctctgtatt
      841 ttttgtctct gagtctgacg gcatgtttta ctttttcatt tgaaaaactg gatatcagca
      901 tacgatgcca gaataaaaat gaacttgaaa aaaactacct attttggatt ttgtgcatag
      961 gaggattgat atgccgacat atgaatacaa atgcagtaca tgcagcacag tgtttgaaca
     1021 ttttcagtcc ataaaagacg agcctctcaa agaatgcctc tgcgagaaaa aaggttctgt
     1081 cagcagactg atttccagcg gaacagggat tattttcaaa ggctccggtt tttacgtaaa
     1141 cgattacaaa aaatcaggtt cagaaaagtc cagcgattcc tcatcttcat ccaccagctc
     1201 agcatcatcc gggtcctgat ttttgaacag gctcggaata atcgctgga
//
LOCUS       DAOQGJ010001545         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90029_length_1249_cov_3.022613, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001545 DAOQGJ010000000
VERSION     DAOQGJ010001545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90029_length_1249_cov_3.022613"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PK683_19500"
     CDS             <1..1021
                     /locus_tag="PK683_19500"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10694.1"
                     /translation="IKAADAFLQFVFITGVSKFSKVNLFSGLNILQDITLDERYATIC
                     GYTESELSVFEERMTDVNRNEMRRWYNGYNFMGESVYNPFDILLFLDSKKYKNYWCQT
                     GNPGFLIDLIKKRKYDPVNIEFIRLSEEALNSFDVDSILLESILFQTGYLTVKEYFSN
                     TEDSYYTLKYPNLEVKKSLSNTILEFMHSASISDLIGSKNDLREAVKAGEMDKVRICI
                     HSFFASIPHDWYRNNPIAEYEGYYCAVFYCYMNALGFDVQTEVPTNLGQVDIAVFYHD
                     KIYIFEFKTAATQGSVSALQQIKDKKYYEKYVSDSIKNIYLIGIEFSPVDRNIVGFEW
                     EVMNI"
BASE COUNT          413 a          220 c          239 g          377 t
ORIGIN      
        1 cattaaagca gcagatgctt tcctgcagtt tgtatttatt accggagtct ccaaattctc
       61 taaagtaaat ctgttcagcg gactcaatat tctgcaggac attacacttg atgaacgata
      121 tgccacaata tgcggatata ccgaatctga actctctgtc tttgaagaga gaatgactga
      181 tgtgaaccgt aatgaaatga gacggtggta taacggatac aactttatgg gtgaatcagt
      241 atataatcca tttgatatac tgctgttttt agattcaaag aaatacaaaa actactggtg
      301 ccagacagga aatcccggat ttctgatcga cctgattaaa aaacggaaat acgatccggt
      361 aaatattgaa ttcatccggc tgtctgaaga ggctctcaac tctttcgatg tagattctat
      421 tctgctcgag tccatacttt ttcagaccgg ttatctgact gtgaaagaat atttcagcaa
      481 tactgaagac agctattata ctctgaagta tcccaatctg gaagtaaaaa aaagtctctc
      541 aaatacaatt ttggaattta tgcacagtgc ttccatttca gatctcattg gatcaaaaaa
      601 tgatctgaga gaggctgtga aagcaggcga aatggacaaa gtccggatct gcatccattc
      661 ctttttcgca tctattcccc acgactggta cagaaataat ccgatagctg aatatgaagg
      721 gtattattgt gcagtgtttt actgttatat gaatgcactt ggatttgatg tgcagacaga
      781 agtgccgaca aacctcggtc aggtggatat tgcagttttt taccatgata aaatatatat
      841 atttgaattc aaaaccgcag caacacaggg cagtgtatcc gcattacagc agataaagga
      901 taaaaaatat tatgaaaaat acgtatcaga ttctataaaa aatatttatc tgatcggaat
      961 tgaattctct cctgtggatc ggaacatcgt cggattcgag tgggaagtga tgaacatata
     1021 gtataaaaga ttttcaaaga taagcttata taaaaatcag gttttcttgc gtatctgtat
     1081 taggtaattt ccctaattaa tgaaattgcc tatttcaaaa tttattcttc agtcagataa
     1141 ttataaagaa agaggtccaa tgaaatctct cctgctgata attatatttt ccaactgtgt
     1201 cacatatatt cccaagcttc ctctgacgga agaggcgcgg gaaagaaat
//
LOCUS       DAOQGJ010001546         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90045_length_1249_cov_2.737856, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001546 DAOQGJ010000000
VERSION     DAOQGJ010001546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90045_length_1249_cov_2.737856"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PK683_19505"
     CDS             complement(<1..360)
                     /locus_tag="PK683_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10695.1"
                     /translation="MMDDLSFTEEFLLSLQNFEKRRRAELFKDYSLTVFLSVCSRYPI
                     RGSSIRISVAGTNGKGSTSFLLKETALKKSGFKNVGLYTSPHLLHRNERIQMNFEQID
                     ESWFESFLRKEKENLPDL"
     gene            complement(437..508)
                     /locus_tag="PK683_19510"
     tRNA            complement(437..508)
                     /locus_tag="PK683_19510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(473..475),aa:Arg,seq:ccg)
     gene            complement(630..800)
                     /locus_tag="PK683_19515"
     CDS             complement(630..800)
                     /locus_tag="PK683_19515"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNH10696.1"
                     /translation="MEIHIVLRMAGAAFLGGGAGFLYHKIAGCRTGACPITANPWIST
                     LYGAFAGLVMAK"
BASE COUNT          306 a          294 c          259 g          390 t
ORIGIN      
        1 aagatccgga agattctctt tctcctttct cagaaaagac tcaaaccagg attcgtctat
       61 ctgttcaaaa ttcatctgaa tcctttcgtt tctgtgcaga agatggggag atgtataaag
      121 tccgacattt ttgaacccgg atttcttcag agcagtctcc ttcagtaaaa aagaagtgga
      181 gccttttccg tttgttcctg cgacagaaat ccgtattgaa cttcctctga tggggtatct
      241 gctgcagacg ctcagaaaaa cagtcagact gtaatcctta aaaagctctg ctcttcttct
      301 cttctcaaag ttctgcagag acagaagaaa ctcttctgtg aaactcaggt catccatcat
      361 tcgggttcag tcatcttcag tgtgggaata gagctactga atcggatttg tgttttttga
      421 aaatgatttt ttgatatgct tctaccggga ttcgaacccg gatatcaggt tccggagacc
      481 tgcactctat cctttgagct atagaagctt atggccattt ttttgcacag gcagacagga
      541 ttcaaacctt ttccatggag ttttttgcct gcagatctga atctgacagt atcggtccat
      601 tccttttttt cgactgttcc ggtctgtgct tattttgcca ttacaagtcc tgcaaacgct
      661 ccgtaaagcg tgctgatcca tgggtttgcc gtgattggac aggcacctgt ccggcacccc
      721 gcgattttat gatacagaaa cccggctcct ccgccgagaa atgcagctcc ggccatcctg
      781 agaacaatat gaatttccat aaattcctcc ttgtctgacc cagtttggca aagcatcact
      841 gccggtcaat aatataccat actgggtatg gattattgct gaccgttcac agatgaatga
      901 gcttcagccg atccgaatca gtgactgctg aaatatatta tccagatttc caatctcgct
      961 acgctccgcc ctgcgggcta ttagaaatat agaaaggtgg gatgctttac cggcagcttc
     1021 aatgcttgcg ctcgtttaaa ggggcttaag cagccccttc gccgttcgga agctgactcg
     1081 gcggctttga aactgcctaa atccgtattt ggcttctgat gaaagataat ttcttggttt
     1141 tttcaacaaa tctactctcc cttcagtccg gtccgttaaa tggaattgtc ttacatgaca
     1201 gaatatttta taatgaatgt tccttttttt ttccggttct ctgcctgaa
//
LOCUS       DAOQGJ010001547         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90215_length_1248_cov_2.290863, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001547 DAOQGJ010000000
VERSION     DAOQGJ010001547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90215_length_1248_cov_2.290863"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1248
                     /locus_tag="PK683_19520"
     CDS             <1..>1248
                     /locus_tag="PK683_19520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH10697.1"
                     /translation="LDGSAVSGLAFSLCLSGSFFKLIEIQKRFSDLTDAFDAFSIFEE
                     MKGNMEKSRMYSRTDLERKKDSMFSERIKKKLIQSLKKQLTFFESVRQLEEKKEFSSA
                     EKMLESSESSSSEISEFRKERIKFLHSVHLFESGQYEEADEKLKSADSKYTRMISEFR
                     KTERIISSYKILKKYFANIKKADQFYSEKKWKAAYQEYEPVYRKSGISAVLVRSADCL
                     FQAGEREKAILLLKKELTDKKDDTEILEALGIFHSELGQNDAAVKYLQSALNEDTSDR
                     ADYHLGIIYLKTDPELSLKHFSRAFALKQSSVYKAAESAALFRLGRQSEAVEVLLSAR
                     KMKDWSSLSETAQAVLLFKSGEYSEAVRMFGKILKTDPSSSDSHYYLSFITESKNSEA
                     AVSYAEKAYAAAPEKFAYRERLI"
BASE COUNT          396 a          229 c          280 g          343 t
ORIGIN      
        1 ccttggacgg ctctgctgtt tccggacttg ccttcagtct ctgtctttcc ggaagttttt
       61 tcaagctgat tgaaattcag aaaagatttt cagatctgac ggatgcattt gatgcatttt
      121 ctatttttga agaaatgaaa ggcaatatgg aaaaaagcag aatgtacagc agaactgatt
      181 tggaaagaaa aaaggattca atgttttcag aaaggattaa aaagaaactg atccagtctc
      241 tgaaaaagca gcttacattc ttcgaatcag tccgtcagct tgaagaaaaa aaagaattca
      301 gcagcgcgga aaaaatgctt gagtcatccg agtcaagttc ctctgagatt tctgaattca
      361 gaaaagaaag gattaaattt ctgcattcag ttcatctttt tgaaagcggt cagtatgagg
      421 aagcggacga aaagctgaaa tctgcagatt ctaaatatac tcggatgatc tctgaattca
      481 gaaagacaga gcgaattatt tcatcataca aaatacttaa aaaatatttt gcgaatatca
      541 agaaagcaga tcagttctat tctgaaaaaa agtggaaggc ggcttaccag gaatatgaac
      601 cggtttaccg taagagcgga atatctgctg ttctcgtcag gtccgcagac tgcctttttc
      661 aggcaggtga gcgggagaag gcgattctcc ttctgaaaaa ggaactgaca gataaaaaag
      721 atgatacgga gattcttgaa gcacttggga tctttcattc cgaactggga cagaatgatg
      781 cagctgtgaa gtatcttcaa agtgcactga atgaagatac ttctgaccgg gctgactatc
      841 accttggaat catatatctg aagacagatc ctgagctttc actgaagcat ttttccaggg
      901 cgtttgcatt gaaacagtct tcagtctaca aggccgctga atctgctgct ctgtttcgac
      961 tgggaagaca gagtgaagca gtcgaagttc ttctgtctgc cagaaaaatg aaggactgga
     1021 gcagtctttc tgaaactgct caggcagttc tgctcttcaa atcgggagag tattctgaag
     1081 cggtgagaat gttcggaaaa atactgaaga ctgatccgtc cagttcagat tctcattatt
     1141 atctttcatt tattaccgaa tctaaaaatt ctgaagctgc agtttcctat gcagaaaagg
     1201 catatgcggc cgctcctgaa aagtttgcat acagagaaag gctgattc
//
LOCUS       DAOQGJ010001548         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90245_length_1248_cov_1.822297, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001548 DAOQGJ010000000
VERSION     DAOQGJ010001548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90245_length_1248_cov_1.822297"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PK683_19525"
     CDS             complement(<1..716)
                     /locus_tag="PK683_19525"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH10698.1"
                     /translation="MYAYDPEHTAADNKLPKEIELLKPVNEIGQKVNLDLEFLNEQGK
                     PVRLKEIFSKGKPVIISMVYYKCPTLCNFHLSTVLNTFKGLDWAVGDKFEYLAVSFDP
                     KETFEVASPKRDAFFDEYQKTVKNSSKEGWHFWTGTEQNSEVLAAQLGFRYSWSEKNK
                     QWMHPSVMYILTPEGKISQYFQGLDLKVRDIRFALIEASSGKVGNFVDKFVLFCYQFD
                     PGQNKYVIYAHNIMRLGAVLT"
BASE COUNT          386 a          267 c          220 g          375 t
ORIGIN      
        1 gtcaatacag caccaagcct cataatatta tgagcatata ttacatattt attctgtcca
       61 ggatcaaact ggtaacagaa taatacaaat ttatcaacaa aatttcccac ttttccgctg
      121 cttgcttcaa tgagagcaaa ccggatatct cgcactttca gatccagacc ctgaaaatac
      181 tgggaaattt ttccttcagg agtcagaatg tacatgactg aaggatgcat ccactgttta
      241 tttttttcgc tccaggaata tctgaagccc agctgggcag caagaacctc cgaattctgt
      301 tcggtgccgg tccagaaatg ccagccttcc ttgctggaat ttttcacagt tttttggtat
      361 tcatcaaaaa aagcatctct cttgggagag gcgacttcga atgtttcttt tggatcaaag
      421 ctgactgcca aatattcaaa tttgtctccg acagcccagt ccagcccctt gaaggtattc
      481 agcacagtgc ttaaatgaaa attgcagaga gtgggacatt tgtagtatac catactgatg
      541 ataacgggtt ttcccttgga aaatatctcc ttcagccgga caggttttcc ctgttcattg
      601 aggaactcca gatccagatt gaccttctgc ccaatttcat tgacaggctt gagaagttct
      661 atttctttag gaagcttatt gtctgctgca gtatgttcag gatcgtatgc gtacaatgag
      721 acacagaacc aaaaagctgc aaaaaaaacg atccttaatc cgtagaaatt tttattcatt
      781 gagataggtc agagtttatt ttttttgaac agaagcggct ggtttatcca ctccgttcac
      841 tccggaatga aaaaaggcga tataaccgaa aaacagtacg ttaaatgcca aaatgacaag
      901 acaaacaatg agtccttgtt gattgtgctc tttcataaac cctctataga ccttattgga
      961 aacttgtaaa aaacagtcaa ttgtatttcc caaatttccc gaactgcgca ttttcttcaa
     1021 tagaaaatta ttttttgctc cgttttcagt actgagttag agaactagaa tcaaattaaa
     1081 aaatacataa cttctttcat ttctggcagt cagagtctct gcagaaaaaa gtctttcttt
     1141 aatccttctc cctgccttaa agaagattac aacagtctat aaatcattgt acctgcagac
     1201 agaagccgtg cctaagcata atctaagtaa cactgccttg caaaaaac
//
LOCUS       DAOQGJ010001549         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90322_length_1247_cov_2.785235, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001549 DAOQGJ010000000
VERSION     DAOQGJ010001549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90322_length_1247_cov_2.785235"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..786)
                     /locus_tag="PK683_19530"
     CDS             complement(70..786)
                     /locus_tag="PK683_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004757601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH10699.1"
                     /translation="MKKILVIEDDQDIGLLLKRILDSSHYDTRVSPTAEIGLKSYKEE
                     RPDMLILDLSLPDQDGIDVCRNIRRMDDKVPIFILTARNEEIDRIMGLELGADDYITK
                     PFSIRELKARVDSFFRRWDNLTGKTVQSYSVMSSETELIRGSMKIDRARRRISVGDII
                     LNISRKEFDIIFMLASNPGKVFSREMILREVWGKDWNGYERMIDSHIKRIRSRFEKAE
                     VKSDWVETIWGVGYRFTDIY"
BASE COUNT          354 a          268 c          196 g          429 t
ORIGIN      
        1 ccgacaaaat ctcccagtgc ataccagccg atgcacctgt tatttgccga ccatgcaggc
       61 ttccaggaat taatagatgt cagtaaatct atatccgaca ccccagatag tttctaccca
      121 gtctgatttt acttccgctt tttcaaatct ggatctgatt ctctttatat gactgtctat
      181 cattctttca tatccgttcc agtctttgcc ccatacttct ctcagaatca tctctctgga
      241 aaaaactttc cctggatttg aagcaagcat aaatattatg tcaaactcct ttctggatat
      301 attcaggatg atgtctccta cagagattct tcttcgggcc ctgtctattt tcatggaacc
      361 gcgaatcagt tctgtttcag aactcattac tgaataagac tgaaccgttt tcccggtaag
      421 attgtcccat cttctgaaga agctgtcgac tctcgctttc agttcacgaa tggaaaaggg
      481 ttttgtaata taatcatcag cacccagttc gagtcccata attctgtcta tttcttcgtt
      541 tcttgcagtg agaatgaaaa taggcacttt gtcatccatt cttctgatgt ttctgcagac
      601 atcaatccca tcctggtcag gcagagaaag atcgaggata agcatatccg gacgttcttc
      661 cttgtaggat tttaggccga tttcagcagt tggagaaacc cttgtatcat aatgagaaga
      721 atctaaaatt cttttcaaca gaagtcctat atcctgatca tcttcgataa ctaaaatttt
      781 tttcataaac cctctaaatt ttctgtcgcg gatcagttat gttttttgaa acattcataa
      841 tgcggatatt ttcaaaaact tgaatatatt cacacttact atcagtccgc tgtcttataa
      901 aacacacatc cagacagttc agtctattcc tgctttctct tcaggcattc tgctgattat
      961 ctgaaaatta aattcatttt tttatcagta aatacttttc tgctattaag tcaagaaaat
     1021 catatttcat agcagtcttt ttttgagatt gctgcacttt tatcatttaa ttaaaagatt
     1081 tgattttgat tgattttcag gcgaaaaatc ataaaccata caggattcat gccgtttcac
     1141 tgccaaagtc aaggttctct gcagatgtta aaaattgaaa tttccaaggc ctgcaagtac
     1201 ggaaataatt aaaaactgaa agttttcccg ctgattttcc ttggaaa
//
LOCUS       DAOQGJ010001550         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90331_length_1247_cov_2.687081, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001550 DAOQGJ010000000
VERSION     DAOQGJ010001550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90331_length_1247_cov_2.687081"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PK683_19535"
     CDS             <1..878
                     /locus_tag="PK683_19535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNH10700.1"
                     /translation="HMEEYPFVHGNYLYFTRYFYGEVKEAKIYRSRFNGKEFLEPEAL
                     PSPVNLEGSANFAAVISPDGKYIYYSSDRKGGYGGTDIYRSEIHQDGTYGAAENLGPE
                     INTAGLESYLVINPADGSFIFARKEGEKSYDLYSSLPPEKKPEIKIAQPTVTEPPPKK
                     DGPITKDNLENIPEILKQRKKLSLSAIQFEINSSEILSDSKPFLDKVADYLTSNKELK
                     IRVTGHTDLTGDLDYNKKLSLDRAESVKEYLHTKGVAVGKISIQGKGSSEPIIPDTKI
                     ESNRMNRRVEFEIVD"
     gene            complement(883..>1247)
                     /locus_tag="PK683_19540"
     CDS             complement(883..>1247)
                     /locus_tag="PK683_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10701.1"
                     /translation="DPGFSLKKFDIDYWIVGCFDPPACCKPSFLEEISSVLCFFSLGF
                     FPAVRTEREKASSFAVFDRDFRLLRQFEYRERIWTLAAIWVLWADSLISDPYPPRAYQ
                     NEMNRLTRDLYKLLKTPD"
BASE COUNT          421 a          266 c          266 g          294 t
ORIGIN      
        1 atcatatgga agagtatcct tttgtgcatg gaaactatct ttattttaca agatattttt
       61 acggtgaagt gaaagaggcc aagatataca gatccagatt caacggaaag gaatttcttg
      121 aaccggaagc acttccatcc cccgtcaatc tggaaggttc cgcaaatttc gctgcagtca
      181 tttctccgga cggtaaatat atttattatt catcggacag gaagggaggt tacggaggaa
      241 ctgatattta cagatccgag atccatcagg acggtacata cggtgcggcg gaaaatctag
      301 gaccggaaat caatacagcg ggacttgaat cctatctggt gatcaatcct gcagacggaa
      361 gtttcatatt cgccagaaaa gaaggagaaa aaagttatga tctctactcc tctcttcctc
      421 ctgaaaaaaa gccggaaatc aaaatagcac agccgacagt cacagagcct cctccaaaaa
      481 aggacggacc gattacaaaa gacaatctgg aaaatattcc tgaaatcctg aagcagagaa
      541 aaaaactgag tctgtctgca attcagtttg aaatcaattc cagtgaaata ctttccgatt
      601 caaaaccgtt tcttgataaa gtagccgatt atctgacatc caacaaagaa ctcaagatca
      661 gagtcacggg acatacagat ctgactggcg acctggacta taacaagaaa ctctcattag
      721 acagagcaga atcagtgaaa gaatatcttc atacaaaagg agtggctgtc ggtaaaatat
      781 ccattcaggg aaaggggtca tcagaaccga tcattcctga tacaaaaatt gaatccaaca
      841 gaatgaacag aagagtcgag tttgaaatag tggactgaaa aatcagtccg gggttttaag
      901 aagcttataa agatcccttg tcagtctgtt catttcattt tggtatgcac gcgggggata
      961 cggatcactg atcagactgt ccgcccagag tacccagatc gcggcgagag tccagattct
     1021 ttccctgtat tcaaactgcc tgagcagtct gaagtctctg tcaaagacgg caaatgatga
     1081 tgccttctcc ctctccgttc tgacagccgg aaaaaatccc aatgagaaaa aacagagaac
     1141 tgaactgatt tcttcaagga aacttggctt acagcaggca ggcggatcaa aacaaccgac
     1201 aatccaatag tcaatgtcaa actttttcag agaaaaaccg ggatcgc
//
LOCUS       DAOQGJ010001551         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90448_length_1246_cov_3.463476, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001551 DAOQGJ010000000
VERSION     DAOQGJ010001551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90448_length_1246_cov_3.463476"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1246
                     /locus_tag="PK683_19545"
     CDS             <1..>1246
                     /locus_tag="PK683_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10702.1"
                     /translation="APHSATAPYGNTFLLIYWHRWSPEKDTLFLGPYYSRENKIVKDR
                     FRTLFPVYWSGESEKKEWSMILPLYINYNDKENDWHFNVFWYSRKGTGAVDPSLNIGK
                     QKDTWYLDSSVTVIYGLFGISLRQPIEKPAFFRSVTERLKSSEEKDQEKKTAEEKAAN
                     DGPGLSKKRTFDREHSREFLGYSALFGLFSYEKGDTKRHVRLLPLAWLTWDEASDEKV
                     TVIPPFPPIFFRYKTEDLEYFVVFPFYGKQRDKDSELSSVLLNMYIAEKHREKNREET
                     SVIWPFFNKYSEDGANGHRLLPFYIQKNTENGTVKESRNYTLFSVYSNYKNEGSERKE
                     FLIWPLLIYWSSENSKVYSSYTLWLTPFFYRSRDEYSARTNVLWFTDWKFQKNRSEKI
                     EKEELSYLMIFPFYMNPQGRTY"
BASE COUNT          398 a          253 c          270 g          325 t
ORIGIN      
        1 ttgctcctca ttccgcgacg gctccttacg gaaatacatt ccttctgatc tactggcacc
       61 ggtggagccc ggaaaaggat actctgttct taggaccgta ttattcgagg gaaaacaaaa
      121 tcgtcaagga caggttcaga actctctttc cagtctactg gtcaggggaa tccgaaaaga
      181 aggaatggag catgattctg ccattatata taaattataa tgataaggag aacgactggc
      241 attttaatgt tttctggtat tccagaaagg gcacaggagc agtggatcct tctctgaaca
      301 ttggaaagca gaaagacacc tggtatctgg attcatcggt cacagtcatt tacggactgt
      361 tcggaatcag cctcagacag ccgatagaaa agcctgcatt cttccggtcg gtgacagaga
      421 gactgaaaag ttcagaagag aaggatcagg aaaagaaaac tgcagaagaa aaagcagcaa
      481 atgacggccc ggggctttcc aaaaaaagaa ctttcgaccg ggaacattcc agagaattcc
      541 tcggatactc agctctcttc ggactgttct cttatgaaaa aggagatacc aaaagacacg
      601 taaggcttct tcctctggcc tggctaacat gggatgaggc atccgacgaa aaagtgactg
      661 tcattcctcc ctttcctccg atctttttcc ggtataaaac agaagacttg gaatactttg
      721 tggtttttcc gttttacgga aagcagagag acaaggattc tgaactgtct tctgttcttc
      781 tgaatatgta cattgctgaa aaacacagag aaaagaaccg ggaagagact tctgtcatat
      841 ggccgttttt caataaatat tcagaagacg gtgcaaacgg tcacagactt cttcctttct
      901 acatacagaa aaatacagag aacggcacag taaaagaaag cagaaattat actctttttt
      961 cagtatacag taattataaa aatgaaggtt ccgaaagaaa ggaatttctc atatggcccc
     1021 tgctgattta ctggagttca gaaaattcaa aagtttattc cagttatact ctctggctta
     1081 ctccgttctt ctacagaagc agagacgagt attctgcgag gacaaatgtt ctctggttca
     1141 cagactggaa atttcagaaa aacagatcgg aaaaaattga aaaggaagag ctgtcctatc
     1201 tgatgatctt tcctttttat atgaaccctc agggcagaac atattt
//
LOCUS       DAOQGJ010001552         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90493_length_1246_cov_2.554156, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001552 DAOQGJ010000000
VERSION     DAOQGJ010001552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90493_length_1246_cov_2.554156"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PK683_19550"
     CDS             <1..920
                     /locus_tag="PK683_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10703.1"
                     /translation="MTLTERHSASKRKREIRKIPSDIRLSPGEYSAEIRGRGQKKDYS
                     FTVYPGRTSVIRYEPYIKDGSVFFYSNASGYSARIGNEQSRIPASFVRMNGFEEKIYV
                     ESEHSGRTSVFLPAEESISRYLVLSGPVSLLSEKNSFIWKTGADTEVETEMKEYLSFI
                     NHTKQFREDWSGKISHYFVPENAELSGGLFETQEMGQGTCFLGISSGTHWYGFIAEED
                     RISVIDTLSSPKPAASYRILSAEGKEKRKLSFRISRAENRIELLIAGEQVHSSWINPD
                     LPFRIGAYSKGAIFNRISFLHSLEIQYDF"
     gene            910..>1246
                     /locus_tag="PK683_19555"
     CDS             910..>1246
                     /locus_tag="PK683_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10704.1"
                     /translation="MISEKINVSFYHFFISSLRRLLPAVLLYLIFGCSGTEAFRKSIV
                     HETVSPENSLFQEFFRENCTAVIEMYSKIPDNKKNISVRKAAAECFLETGRKKEYLAE
                     WKKIASSDVR"
BASE COUNT          380 a          242 c          280 g          344 t
ORIGIN      
        1 ggatgactct gaccgaaaga cattctgcat caaaaagaaa gagggagata aggaaaattc
       61 cctcggatat ccgtctttct cccggtgaat acagtgcaga gatcagaggg agaggtcaga
      121 agaaagatta cagtttcact gtttatccag gaagaacttc tgtcatccgt tatgaacctt
      181 atattaaaga cggatcggtt tttttttaca gcaatgcttc cggatactct gccaggattg
      241 gaaatgaaca atcccgaatt ccggcgtctt ttgtcaggat gaacggtttt gaagaaaaga
      301 tttatgtgga atcagagcat tctggaagga cttccgtttt tctgcctgca gaggaaagca
      361 tttccagata ccttgtgctt tcaggaccgg tcagtcttct gtctgaaaaa aacagcttta
      421 tatggaaaac aggtgcagac actgaagtcg aaacagagat gaaggaatat ctcagtttca
      481 taaaccatac aaaacagttc cgtgaggact ggtctggaaa aatcagtcat tattttgttc
      541 ctgaaaacgc tgagctcagt ggaggtcttt ttgaaaccca ggaaatgggg cagggtacat
      601 gctttttagg aatcagctct ggaactcact ggtacggttt cattgcagaa gaagacagga
      661 tttctgtcat agatacactt tcttctccta agcctgcagc ttcttaccgc atcctctcag
      721 cagaaggaaa ggaaaaaaga aagttatcct ttcgtatcag cagagcagag aacagaattg
      781 aacttctgat tgcaggcgag caggtgcatt cttcatggat aaaccctgat ctgcctttca
      841 ggatcggtgc atactcgaaa ggagcgatat tcaaccgcat cagttttctt cattcactgg
      901 aaattcagta tgatttctga aaaaataaat gtatcctttt atcatttttt tatatcctct
      961 ctccgcaggc ttctgcctgc ggtgctcctg tatctgattt tcggctgcag cgggacagag
     1021 gctttcagaa aaagcatagt tcatgaaaca gtgagtccgg aaaattctct ttttcaggaa
     1081 tttttccgtg aaaactgtac ggctgtaatt gaaatgtaca gtaaaattcc tgataataaa
     1141 aaaaatatat cagtcagaaa ggctgcagcg gaatgcttcc tggaaaccgg aagaaaaaaa
     1201 gagtatttgg cagaatggaa gaaaatcgca tcttccgatg tacgcg
//
LOCUS       DAOQGJ010001553         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90526_length_1246_cov_1.989924, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001553 DAOQGJ010000000
VERSION     DAOQGJ010001553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90526_length_1246_cov_1.989924"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1246
                     /locus_tag="PK683_19560"
     CDS             <1..>1246
                     /locus_tag="PK683_19560"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNH10705.1"
                     /translation="SYELKDAKDLKAVQNQEFYTSPDPVIQRIKTGDFDRKTFLKLMG
                     ASAAMATVNCLPQPKKKIVPYAEKPEFMTPGLSNYYASVCGGCESSCGILVKTKDGRP
                     LKLEGNSKHPISKGGMCAQGQAGILNLYDPDRSREPAKLAAGVPSKITWKDLDTAVQK
                     AIQDNKGKLRIVTLPLKSPTSKKLISEFLTKAGGGKHYEIDVLSPEDAIAEASLLNYG
                     KAIVPNYRFDKAQVVFSLDADFLGTWISPVEFSKDFALGRKLKAGAESMNQLIMAETM
                     PTVTGSNADLRIPVRPGDLKKLALALAKALSELGANANGLPAIDVDASAKEIDIKAEV
                     IRTAAKKLWAAKGSSLVVGNSLGADAVEIQVLVNLLNTMLDNDGKSVDHMANRGEAPN
                     YSKNLAALKADLNSGEAAVVLIY"
BASE COUNT          384 a          280 c          284 g          298 t
ORIGIN      
        1 atcctacgaa ctgaaagacg caaaagatct gaaagcagtc cagaatcagg aattttatac
       61 ctctcctgat cctgtaattc aaaggatcaa aacaggggat tttgacagaa agacctttct
      121 gaaactgatg ggcgcttctg cggccatggc cactgtaaac tgtcttcctc agcccaaaaa
      181 gaaaatagtt ccttatgctg aaaagcccga gtttatgact cccgggcttt ccaattatta
      241 tgcctctgtc tgcggcggat gcgaatcctc gtgcggaatt ctcgtcaaga ctaaggatgg
      301 acgtcctctg aaattggaag gaaattccaa acatccgatc tccaaaggcg gtatgtgtgc
      361 tcaggggcag gccggtatac ttaatttata tgatccggac agatccagag agcctgcaaa
      421 actggccgca ggagttcctt ccaaaatcac ctggaaagat ctcgatacag cagtccagaa
      481 agcaatccag gacaataaag gaaaactgag gatcgtcact cttcctctga aatccccgac
      541 ttcaaaaaaa ctgatctctg aatttttaac aaaagccgga ggcggaaaac actatgaaat
      601 agatgttctt tctcctgaag atgcaattgc agaagcgtca cttctgaatt acggaaaggc
      661 aatcgttccg aattaccgtt ttgacaaggc acaggttgta ttctctctgg atgcagattt
      721 cctgggaact tggatttctc ctgtagaatt cagcaaggat tttgcacttg gaagaaaact
      781 gaaagcaggt gcagagtcga tgaatcagct gataatggca gaaacaatgc ctacagtgac
      841 cggttccaat gcagacttaa gaatccctgt ccgtcctggt gacctgaaaa aacttgctct
      901 tgcactggca aaagctcttt ccgaactcgg agcaaatgca aacggtcttc ctgcaatcga
      961 tgtggatgca tcagcgaagg aaatcgacat caaggcagaa gtcatcagaa ctgcagcgaa
     1021 aaaactctgg gcagcaaagg gttcatcact tgttgttgga aacagcctgg gtgcagatgc
     1081 tgtagaaata caggttcttg taaatcttct gaatacaatg ctcgacaacg acggaaagtc
     1141 tgttgaccat atggcaaaca gaggagaggc tccaaattac agcaaaaacc ttgctgcact
     1201 caaagcggat ctcaattcag gggaagcagc agttgttctc atttat
//
LOCUS       DAOQGJ010001554         1245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90588_length_1245_cov_3.154622, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001554 DAOQGJ010000000
VERSION     DAOQGJ010001554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90588_length_1245_cov_3.154622"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PK683_19565"
     CDS             complement(<1..840)
                     /locus_tag="PK683_19565"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10706.1"
                     /translation="MSSLFGSAEPPLAERMKPESLDRVTGQDRTVNVLKRLKKPVSLI
                     FYGPPGTGKTSISMILSRNWGLPFRYLNAASAGVKDIKDIAAETERTGSICVFLDEIH
                     RFSSSQQDSLLDSVEKGRIILMGATAENPAFRVNRALLSRCQIFRLYPLERISFFALY
                     ERAVSDFSLGTVLDEHCIDNIINFSGGDARKFLNFLELLDSLDPPEEGWTPESVSEVF
                     SSQVISYDKNKENHYDYISAFIKSVRGSDPDAALLYLAAMLEGGEDPLFIMRRLLILA
                     SEDV"
     gene            complement(837..>1245)
                     /locus_tag="PK683_19570"
     CDS             complement(837..>1245)
                     /locus_tag="PK683_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10707.1"
                     /translation="RLTPDPFYNRFGDEAKKLTDRKDLVVVLDNTPRTEVFYFSDRRG
                     FQLIMPGVFQFALSTVPPEIKTKYISELEDYRSKGAKYFFTPYGEFAVYIPWFKQYLD
                     EKYECLLGCNVTPEMVVRKDPSIKGFIYRLSEK"
BASE COUNT          335 a          306 c          270 g          334 t
ORIGIN      
        1 aacatcttcg ctggcaagaa taaggagccg cctcatgata aacaggggat cctcgcctcc
       61 ctcaagcatt gcagcaagat acagaagggc tgcgtcagga tcacttcctc ttacagactt
      121 tatgaaagca gaaatgtaat cgtaatgatt ttccttattt ttatcataag aaattacctg
      181 tgaagaaaat acctctgaaa ctgattcagg ggtccatcct tcttccggcg ggtcgagcga
      241 gtcgagaagt tccaggaagt tcagaaactt tcttgcatct cctccggaaa aattaatgat
      301 attgtctata caatgctcat ccaggacagt tcccagtgaa aaatcactga cagctctttc
      361 gtagagtgcg aaaaatgaaa ttctttccaa gggataaagt ctgaatatct ggcatctgga
      421 aagaagcgcc ctgttcactc gaaatgcagg attttcggca gttgctccca tcagaatgat
      481 tctgcccttc tccacactgt ccagaagact gtcctgctga gaggaactga acctgtgaat
      541 ctcatcgaga aacacacaga tgctccctgt cctttctgtc tccgctgcga tatccttgat
      601 atccttgact cctgcagagg cggcattcag atatcggaac ggaagtcccc agttccggga
      661 caggatcatg gaaatgctgg tttttcccgt gccaggcggt ccgtaaaaaa tcagtgaaac
      721 cggttttttc agcctcttca gaacattgac tgtcctgtcc tgaccggtca ccctgtcaag
      781 actttccggt ttcatccttt ctgccaaagg aggctcggct gaaccgaaaa gcgaactcat
      841 ttctccgaaa gacggtagat aaaccctttg atggaaggat ccttccttac aaccatttca
      901 ggagttacat tgcacccgag aaggcattcg tatttctcat ccagatactg cttgaaccag
      961 ggaatgtaca ctgcaaattc tccgtaaggg gtaaagaaat actttgcacc cttggatctg
     1021 tagtcctcca gttcggaaat gtactttgtc ttgatttcag gaggaactgt gctcagagca
     1081 aattgaaaca ctccaggcat aatgagctgg aatccccttc tgtcactgaa gtagaacacc
     1141 tctgtacggg gagtattgtc cagaaccacg accaggtctt ttctgtctgt cagctttttg
     1201 gcttcatcac cgaacctgtt gtaaaaagga tcaggtgtca gtctc
//
LOCUS       DAOQGJ010001555         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90715_length_1244_cov_3.391085, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001555 DAOQGJ010000000
VERSION     DAOQGJ010001555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90715_length_1244_cov_3.391085"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PK683_19575"
     CDS             complement(<1..243)
                     /locus_tag="PK683_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNH10708.1"
                     /translation="MSELENQEVKETFPTKERSGGRRHEEDDEDDEKGLRGKDGDSKF
                     KKNSKYKRKVCKFTADKALAAALNYKRVDILERFITN"
     gene            complement(251..529)
                     /gene="rpsF"
                     /locus_tag="PK683_19580"
     CDS             complement(251..529)
                     /gene="rpsF"
                     /locus_tag="PK683_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNH10709.1"
                     /translation="MRAYEITAVLQESPASLVDETKNTIKEFFKKYSVEMTEEEDWGS
                     KKLWHTIRGLETGHYSHFKCKAEPSAIAKLENEFKLNQNILRSLVIKV"
     gene            complement(969..>1244)
                     /locus_tag="PK683_19585"
     CDS             complement(969..>1244)
                     /locus_tag="PK683_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10710.1"
                     /translation="IFHSPPPAEETPSCSESGVLGSIAGIAGSIQASECLKHFLGMET
                     TLGNILFFDAKTMEFRKRKFPKNPECLLCGKKTMKDYSWWEEDFCRD"
BASE COUNT          302 a          294 c          237 g          411 t
ORIGIN      
        1 gtttgtaatg aatctttcca gaatatccac tcttttataa ttcaatgcag cagcaagagc
       61 cttgtctgca gtaaacttgc atacttttct cttgtatttt gagttcttct tgaatttaga
      121 atcaccgtct tttccacgaa gacctttttc atcatcttca tcgtcttctt cgtgtcttct
      181 tccgccggat ctttccttag ttgggaatgt ttcttttact tcctgatttt ccagttctga
      241 cataatcacc tcaaaccttt atcactaatg atcttaaaat gttctgattg agcttgaact
      301 cattttccag ttttgctatg gcagaaggct ctgccttgca tttaaaatgg gaatagtgtc
      361 cagtttccaa tccgcgaatt gtatgccaga gttttttgga accccagtct tcctcttcag
      421 tcatttcaac tgaatacttc ttaaaaaatt ctttgattgt gttcttggtt tcatcgacga
      481 gtgacgccgg tgactcctga agaacagcag ttatttcata tgcgcgcatt ttgctctcct
      541 ttgggtattc ccatagttcc gcataagtcc gaggccgcag ttcggtacgg tatgggtaga
      601 agttactatg taaacttaac ttcttacagc ttctgttttt ttgagcctac cggcagggct
      661 gtgtcccaaa atatcaggtt aagaacagca ggcgacattt tgcagctcgc tgcgcgagct
      721 gagttatatc ctatcggaaa attaagggac tgccgtccct tgaaaatccc tgccaactgc
      781 tatcctgaca gcatcagact atgtccctcg aaaatcccca aaaaatcctt agccgcttcc
      841 gaattagcga gaggtttgca accctcgcaa gtctttatgc cgctaagacc tcgccttggt
      901 attaaaaagc cagtttacat tattgaccat tttttaatca tgaagccctg tgtcaagatc
      961 ggaatttttc agtctctgca gaaatcctct tcccaccagg aatagtcttt catagttttt
     1021 ttcccgcaga gaaggcattc cggatttttg ggaaatttcc tttttctgaa ttccatcgtt
     1081 tttgcatcaa aaaacagaat attcccgaga gtcgtttcca ttcccagaaa atgcttcaga
     1141 cattcagatg cctgaatgct ccctgcaatt cccgcaatgc tgccgagaac tcctgattcg
     1201 gaacagctgg gggtctcttc agctggaggg ggggaatgga aaat
//
LOCUS       DAOQGJ010001556         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90739_length_1244_cov_2.746005, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001556 DAOQGJ010000000
VERSION     DAOQGJ010001556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90739_length_1244_cov_2.746005"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PK683_19590"
     CDS             complement(<1..1084)
                     /locus_tag="PK683_19590"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNH10711.1"
                     /translation="MKSVGILGGGAAGFFAAVQLAELAKGSVKITILEKGNEPLAKVK
                     ISGGGRCNVTHHLFDPELLSQKYPRGSKELRAAFARFQPKDTIEWFAKRGVRLKTEKD
                     GRMFPETDSSSTIINCFMKEIEHYNISLKLNSPIQSVFVQKDEQEKRFRLTGPEGGEL
                     FFDFLLVATGSNRKVWGWMEALGHSIESPVPSLFTLNVPNSRMSSLPGTSVPNAEIKI
                     HPKGKPQNGPLLITHWGFSGPAVLRLSAWEARTLAECEYRTKIRINWTGNYNGEEYIS
                     YLQSLRKEFPSRYISSTPTEGFSARLWETFLKNCEISPEKRWSEISNKELNQLREEVT
                     NGIFDIDGKAVFKEEFVTCGGIRRKEV"
BASE COUNT          316 a          286 c          253 g          389 t
ORIGIN      
        1 ccacttcctt tcgtctgatt cccccgcagg tgacaaactc ttccttgaat actgcttttc
       61 cgtcaatatc aaagattcca tttgtgactt cttctctgag ctgattcagt tctttattgg
      121 aaatttcaga ccaccttttt tcaggagaga tttcacagtt tttcaggaat gtttcccaaa
      181 gtctggcaga aaatccttct gtcggagtgg aagaaatata tctggaagga aattctttcc
      241 tgagagactg taagtaggat atatactctt ctccattata attccctgtc cagttgattc
      301 tgatttttgt tctgtattcg cattctgcga gagttctggc ttcccaggca gagagacgga
      361 gcactgccgg tccgctgaat ccccagtgag taatcaacag agggccgttc tgcggttttc
      421 cctttggatg aattttgatc tctgcatttg ggactgatgt tccgggaaga ctgctcattc
      481 tggaatttgg aacattcagt gtaaaaaggg acggaaccgg agattcaatt gagtgcccta
      541 atgcctccat ccagccccat acctttctgt ttgatccggt ggcaacaagg agaaaatcaa
      601 agaacagctc ccctccttct ggaccggtca gtctgaatct tttttcctgt tcatccttct
      661 gaacaaaaac agactggatc ggtgaattca gtttcaagga tatattataa tgctctatct
      721 ctttcataaa acagtttata attgtagaag atgaatctgt ttcaggaaac attcttccgt
      781 ctttttctgt cttgagtcgg actcctctct tcgcaaacca ttcaattgtg tccttgggct
      841 gaaatctggc aaatgctgcg cggagttcct tggaccctct gggatatttc tgtgagagaa
      901 gttcaggatc aaacagatga tgggtcacat tgcatctgcc tccccctgaa atcttcacct
      961 ttgcgagagg ttcatttccc ttttcgagga tagtaatttt tacacttccc tttgcaagtt
     1021 cagccagctg aactgctgca aaaaaaccgg ccgcgccccc gccaagaatt cctactgatt
     1081 tcattggtcc agatttctca atgcaagcat tatccggaaa agcagctcga aacttttgtc
     1141 aattgcatcg gaacttccca gattcattat gatatgagta tcatacttgg gagagtaaaa
     1201 catatgtgta gataatatcc cagaatgtcc ataaagctca ggag
//
LOCUS       DAOQGJ010001557         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90741_length_1244_cov_2.742641, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001557 DAOQGJ010000000
VERSION     DAOQGJ010001557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90741_length_1244_cov_2.742641"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PK683_19595"
     CDS             complement(<1..512)
                     /locus_tag="PK683_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNH10712.1"
                     /translation="MAKLVFKPMQIAEVHDQIEISMSEKYKKYHKAETEEEYEADAEG
                     KVIEQYSGPSIEEIEEEIARYRAEAEEQVRIMLADAQSKAEKIIEDGKTRAFQLIQDS
                     KEKVKIEEDAAKAKADQIKDRAKQEVERTMKEAEMKVAEIEHDAYQRGYDAGREVGFK
                     KGQAEVRRLI"
     gene            complement(513..>1244)
                     /locus_tag="PK683_19600"
     CDS             complement(513..>1244)
                     /locus_tag="PK683_19600"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HNH10713.1"
                     /translation="DKKGTEKKYSTRLDYSSPLESLKKKKRDELELIIRDEPARMIAM
                     VMCFADPDEASHLIEDFPESMRDKIIEEIRKVDFYSESLRAELESFLFFKYDLIESRM
                     IVSKVTNRSGKKVAEILSRISPNLSSRMFSNIKEKDPGFAKSIDEYFYTFQDLLYIGR
                     TSLTNFLAGFHPIVLACAMKGIDASLCEKIFERCEPWLVKQIKLELDSMGPTSLAEIE
                     ESQKAIIENLNRDIETGKVKLWKVK"
BASE COUNT          305 a          302 c          203 g          434 t
ORIGIN      
        1 tcaatcagac ggcggacttc tgcctgacct tttttaaacc cgacttcccg gcctgcatca
       61 taacccctct ggtaagcatc atgttcaatt tcggcaacct tcatttcggc ttccttcatt
      121 gttctttcca cttcctgctt tgcacggtct ttgatctggt ctgcctttgc tttagccgca
      181 tcctcttcaa tttttacttt ttctttggag tcctgaatca gctgaaaagc tctagtcttt
      241 ccatcctcaa tgattttttc tgccttggac tgtgcatccg caagcataat cctgacttgc
      301 tcttctgcct cagccctgta tctggcaatt tcctcttcta tttcttctat ggaagggccg
      361 gaatactgct caattacctt gccttcagca tcagcctcat attcttcttc agtttcagcc
      421 ttatgatatt tcttgtattt ctccgacatg gagatttcaa tctgatcatg tacttccgca
      481 atctgcattg gtttaaatac aagcttagcc atttacttta ccttccatag tttaactttt
      541 ccagtttcaa tatcacggtt caaattttct ataatcgctt tttgagactc ttcaatttct
      601 gcaagagaag ttggtcccat tgaatcgagc tcaagcttga tctgcttgac gagccaaggt
      661 tcacaccttt caaatatttt ctcacataaa gaggcatcaa tccctttcat tgcacaggct
      721 agaactatcg gatggaatcc tgccagaaaa tttgtaagag acgtcctgcc gatatataac
      781 agatcctgaa aagtataaaa atattcatcg attgattttg caaatccagg atctttttcc
      841 ttgatattgg aaaacattct gctcgaaagg ttcggactga ttcgggaaag aatctctgct
      901 acctttttac cgcttctgtt tgtcaccttg gatacaatca tcctggattc aatcagatcg
      961 tatttaaaga aaagaaaact ctccagttca gcacggagtg attcagaata aaaatccacc
     1021 tttctgattt cctcaatgat cttgtctctc attgattcag gaaaatcttc tatgagatgg
     1081 gatgcctcat ccggatcagc aaaacacatc accattgcaa tcattctggc gggttcatcc
     1141 cgaatgatca gttccagttc atctcttttt ttctttttga gactttccaa aggagaggaa
     1201 taatccagtc tggtagaata ctttttttcg gtaccctttt tatc
//
LOCUS       DAOQGJ010001558         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90845_length_1243_cov_3.842593, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001558 DAOQGJ010000000
VERSION     DAOQGJ010001558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90845_length_1243_cov_3.842593"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PK683_19605"
     CDS             complement(<1..737)
                     /locus_tag="PK683_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH10714.1"
                     /translation="MKKVQNRIQFWRDLGFFEKYRKLSDEKAAEAIVRISEDSYWGEL
                     SEDMEELDLAILSLEKKKVWFMEDFYTYGSLFPNVKKMYISALKNLSKISGGGFLPAD
                     PKIRQDGFCDGRSERWVLSFLLEEKREEIIFCGDLEVLTFCWVSRINELIQSRGLRFC
                     VIRDGYGAAAVFCLSMEKAEKLSENVQLLDLQPESFWQDAGDYCSQKGDYEEALLCFE
                     KSLSANPQFWHVYASAALCCDSLKLTE"
     gene            complement(734..1132)
                     /locus_tag="PK683_19610"
     CDS             complement(734..1132)
                     /locus_tag="PK683_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10715.1"
                     /translation="MNQSIDFQTVMQAVIRVIGAVLISAGAYLGYLSFEKARMTLDNP
                     NTLAVWLELEKKFPAPAENSVQVPAKNTAADPDKALLRMYYEVKSYIILFLSYIYLWI
                     LVRISASFIQSGIQILLHEPKPRNKKDENT"
BASE COUNT          350 a          309 c          218 g          366 t
ORIGIN      
        1 ctttcagtca gtttcagact gtcgcagcaa agggcagcac ttgcataaac atgccaaaat
       61 tgaggattgg cagaaagcga tttttcgaaa cacagtaaag cttcttcata atcccccttt
      121 tgactgcaat agtcacctgc atcctgccag aagctttccg gctgcagatc gagaagctga
      181 acattttcag aaagtttttc agccttttcc atagaaagac agaaaactgc agcggcaccg
      241 tatccgtcgc ggatgacgca gaaacgcaga cctctgctct gtatcagctc attgatccgg
      301 ctgacccagc agaatgtcag aacctccaga tccccgcaga aaataatttc ctctctcttt
      361 tcctccagaa gaaaactgag cacccatctt tcggatcgtc cgtcgcagaa accatcctgt
      421 ctgattttcg gatcagccgg cagaaaacct cccccggaaa ttttcgataa attcttcaag
      481 gcacttatat acattttctt tacattcggg aaaagacttc cgtatgtata aaaatcttcc
      541 ataaaccaga ctttcttttt ttccaatgac agaatggcca gatcaagctc ttccatatcc
      601 tcactgagct ctccccaata actgtcttca gaaattcgca caatcgcttc tgctgccttt
      661 tcatccgaca actttctgta tttttcaaag aaacccaagt ctctccaaaa ctgtattcta
      721 ttttgtactt ttttcatgtg ttttcatctt ttttattcct gggtttcggt tcatggagca
      781 ggatctgaat ccctgactga ataaaggacg ctgatatcct gaccagaatc caaagataaa
      841 tataactcaa aaataagatt atatatgatt ttacttcgta gtacatccgc agaagggcct
      901 tgtccggatc tgctgcagta tttttggcag gaacctgtac agaattttct gccggtgcag
      961 gaaacttttt ttccagttcc agccagactg caagtgtatt cggattgtcc agagtcatcc
     1021 tggccttttc aaaactaaga tacccaagat aggctcctgc actgatcaga accgcaccta
     1081 tgacgcggat cactgcctgc attactgtct gaaaatcaat cgactgattc ataaaatctc
     1141 ctctttatac tgatattctg aaattttcgc agattgcata taactaattt ccgatttatg
     1201 cattcagtca aagatcattg aacattgggc aatcagctga cag
//
LOCUS       DAOQGJ010001559         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90888_length_1243_cov_2.647306, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001559 DAOQGJ010000000
VERSION     DAOQGJ010001559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90888_length_1243_cov_2.647306"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1243
                     /locus_tag="PK683_19615"
     CDS             <1..>1243
                     /locus_tag="PK683_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10716.1"
                     /translation="PLQRIPFSYSETGQTALHSIQLFNGSTDNPIQRVQKYRSILFSQ
                     EIYIPSLFSADFIQDRRFTEADFHYAIPSLLFEKCTENDSPEILGYHSAKMFFGLPLC
                     VEQSLPIRWFSTDEENTLSQSAFRMIESVQKKDSEFWRRLWNGVIICILSEEEWIRSL
                     SFKRKTSLWDRMEEILKSKFEHSSGYHDRIIMDGKSLDEWMRSGNIRNFLENLSVSDW
                     VVPGNPEKSPLFSSSVSFGGKMFGIFTEKELMTVSGWISLLKENPSPQWNSSIERKYT
                     YNLNIYIKDFDAHTVFRMPAADSRKIIFHKRKKKSDSLNPRKLMQKLVQKQNFPESVS
                     ESRLYIKNMLMKTEKKMNSGKLKKENLIPFQKDTLQNWLKRRMDEQVNKNLPLSGEFL
                     GKAVSRADAVWVLLQLAPASLA"
BASE COUNT          401 a          251 c          235 g          356 t
ORIGIN      
        1 ttccccttca gagaatccct ttttcataca gtgaaacagg acagactgct cttcattcca
       61 tacagctttt taacggcagt acagacaacc cgattcaaag ggttcaaaaa tacagatcca
      121 ttcttttttc tcaggaaata tacatacctt cattattcag tgctgatttc attcaggaca
      181 gacgtttcac tgaggctgac tttcattatg caatcccttc tctgcttttt gaaaaatgca
      241 cggaaaatga ctcacccgaa atactcggtt atcattctgc aaaaatgttc tttggacttc
      301 ctctctgtgt tgaacagtca cttccgatcc gatggttcag cactgatgaa gaaaatactc
      361 tttctcaatc agcgttcaga atgattgagt ctgtccaaaa aaaggattct gaattctgga
      421 gacggctctg gaacggtgtc ataatctgca ttctttctga agaagaatgg atcagatctc
      481 tctcattcaa aagaaagact tctctctggg acagaatgga agaaattctg aaatccaagt
      541 ttgaacactc ctcaggatat catgatagaa taattatgga tggaaaatct ctggatgaat
      601 ggatgcgctc aggaaatata aggaattttc tggaaaatct ttctgtttct gactgggttg
      661 ttcctggaaa tccggaaaaa agtcctcttt tttcttcttc ggtttccttc ggcggaaaaa
      721 tgttcggcat attcacggag aaagaactga tgactgtctc aggatggatc tctctcctga
      781 aagaaaaccc ttctcctcaa tggaacagtt caatagaaag aaaatataca tataatttaa
      841 atatttacat taaagatttt gatgcgcaca ctgtcttcag gatgcctgcc gccgattcaa
      901 gaaaaatcat ttttcataaa aggaaaaaga aatcagattc actcaatccc agaaaactga
      961 tgcagaagct tgtacagaag cagaattttc cagaatcagt cagcgaatca agactgtata
     1021 taaaaaatat gctgatgaag accgaaaaga aaatgaattc cggaaaactg aaaaaagaaa
     1081 atctgattcc ttttcagaag gacactcttc aaaactggct taaaaggaga atggatgaac
     1141 aggtgaataa aaatctgcct ctcagcggag aatttctggg aaaagcagtc tcccgtgcag
     1201 atgcagtatg ggttcttctg cagcttgccc ctgcttctct tgc
//
LOCUS       DAOQGJ010001560         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90907_length_1243_cov_2.425084, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001560 DAOQGJ010000000
VERSION     DAOQGJ010001560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90907_length_1243_cov_2.425084"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PK683_19620"
     CDS             <1..811
                     /locus_tag="PK683_19620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNH10717.1"
                     /translation="LALCKGAAGIFGNSYALVADAIESTSDVLSSFVVLIGLKISAIP
                     KDENHPYGHGKAEPIAAVIVSLSLIGASIVIAIQSIHGIMTPHELPAPFTLAVLAGVV
                     FTKEFLFRKVLQVGEEVESTAVKTDAWHHRSDAITSAAAFVGISIALFMGKGWESADD
                     WAALFAAFIIAFNAYNLFRPAFSEIMDSAPSKEITEQIRAVAFSVEGVIALHKCYVRK
                     MGFDYFVDLHVLVDAEITVREGHTIGHAVKDAIRKANSRITDVLIHVEPAD"
     gene            complement(808..>1243)
                     /locus_tag="PK683_19625"
     CDS             complement(808..>1243)
                     /locus_tag="PK683_19625"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNH10718.1"
                     /translation="STGPTGFNTPTGIALDSAGGLYVADQNNHRVLYFPAGSTTAARV
                     YGQTVFSGNTAGTTATTMNQPFGVAVDSMNGLYVAEFSNNRILYFPSGSTVPTRVWGQ
                     PTFTTGAFSTTSFGLYNPINLTLSNSGALCVAEYTNKRVLCY"
BASE COUNT          325 a          266 c          307 g          345 t
ORIGIN      
        1 gcttgctttg tgcaagggag ccgcaggaat atttggaaat tcttatgctc ttgtggctga
       61 tgcaattgag tcgacatcag atgtcttaag ttcgttcgtt gtcctgatcg gactcaaaat
      121 ttctgcaatc cctaaagatg aaaatcatcc ttacggtcat gggaaggcag aaccgatcgc
      181 cgcagtcatt gtttcccttt cactgatcgg tgcctccatt gtaattgcaa tacagagtat
      241 acatggaata atgactcctc atgaactgcc tgcccctttt actcttgctg tgcttgccgg
      301 agttgtgttt acaaaggaat ttctgttcag gaaggttctt caggtaggag aagaagtaga
      361 gagcactgcg gtgaaaaccg atgcgtggca ccacagaagc gatgcgatta catctgcagc
      421 ggcatttgtc ggaatttcca ttgcactttt tatggggaag ggatgggaaa gtgctgatga
      481 ctgggcagcc ctgtttgcag catttattat agcattcaat gcatataatc tgttccgtcc
      541 cgcattcagt gaaattatgg acagcgcacc ttccaaagaa attactgaac agatcagagc
      601 agttgctttt tcagtggaag gtgtgatagc tcttcataaa tgttatgtcc gaaaaatggg
      661 attcgattat tttgtggatc ttcacgttct tgtggatgca gagattactg tcagagaagg
      721 acataccatc ggacatgcgg taaaggacgc gatccgaaaa gccaattcca gaatcactga
      781 tgttctgatc cacgttgagc cggctgacta ataacacagg actcttttat ttgtgtactc
      841 cgccacgcag agtgctcctg aattggaaag agtgaggttg attggattat acagtccaaa
      901 agaagtggtt gagaaagcgc cagtcgtaaa agtcggctgt ccccaaaccc ttgtcgggac
      961 tgtgcttccg gagggaaaat acaatattct gttattggaa aactcagcca catacagtcc
     1021 attcattgaa tctactgcaa ctccaaacgg ctgattcatt gtcgtggctg ttgtgcctgc
     1081 agtatttcct gaaaaaacag tctgcccgta aacacgtgct gcggttgtgg aaccggcagg
     1141 gaaatacagc actcgatgat tgttctggtc tgccacatac agtccgcctg cagaatccaa
     1201 agctataccg gtcggtgtgt tgaatcctgt tgggccggta gac
//
LOCUS       DAOQGJ010001561         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90908_length_1243_cov_2.399832, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001561 DAOQGJ010000000
VERSION     DAOQGJ010001561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90908_length_1243_cov_2.399832"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PK683_19630"
     CDS             complement(<1..621)
                     /locus_tag="PK683_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10719.1"
                     /translation="MSSAPIEYRQNPAFLELDRELTRPIPNFKAINEALEKVPPFVIS
                     NAVLNDVEFTYLLDYYLKYMEKSTVQKILQNPLFATEALMELFYCQITAYHKAILQKK
                     PAPDLISSYWTYLSKAEYAVLFRSLLETSDTDAISIMMTKVDIVHLKMLAKSGSEISD
                     RMLSFFKKMGSGIKDMAAKDMSIYDFAFNLAVDKSDMEFLAIMEEYA"
     gene            complement(602..>1243)
                     /locus_tag="PK683_19635"
     CDS             complement(602..>1243)
                     /locus_tag="PK683_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNH10720.1"
                     /translation="VLNGAVMSLIGFFGFMSLISAVVPIEAGMAIILWIGIIIGAQAF
                     ESSPIKHAPAIVLGIFPALAGWGVLLVQSIFNFANGKLGEILAKENIANKYAIVMSDL
                     SPDMMLLPPFSYPLHAMLALNQGFLLVSMFWAAICVAVLERQFIKAGHWCLITSFFSA
                     IGMIHSYRLLGNGIANNFSFPASGEFTVAYVLMGGVFYLTSVLARNQDELSSH"
BASE COUNT          379 a          276 c          253 g          335 t
ORIGIN      
        1 agcatattct tccataatag caagaaattc catatcagac ttgtctacag ccagattgaa
       61 tgcaaaatca tagatgctca tatccttcgc ggccatatcc ttgattcctg atcccatttt
      121 cttgaaaaat gagagcattc tgtctgaaat ctcgcttccg ctcttggcca gcatcttcaa
      181 atgcacaatg tccactttgg tcatcataat ggaaattgca tcagtatcac ttgtttcgag
      241 gagacttctg aaaaggactg catattctgc cttgctcaga tatgtccaat aggaagagat
      301 cagatctggt gcaggttttt tctgaaggat agccttatga taggcagtaa tctgacagta
      361 aaaaagttcc atcaacgcct cagtggcaaa aagaggattc tgaagaattt tctgcacagt
      421 ggatttttcc atatatttca gataataatc taaaagatat gtaaattcga catcattcag
      481 tactgcattc gatatcacaa acggaggcac tttttcaagt gcctcattga ttgccttaaa
      541 gttgggaatc ggcctcgtca gttccctgtc gagttccaaa aaggcaggat tctgtcgata
      601 ctcaatggga gctgagctca tcctgattcc ttgccagtac tgaagtcaga taaaagactc
      661 ctcccatcag tacgtatgcg acagtaaact ctcccgatgc aggaaaactg aaattgtttg
      721 caattccatt tccaagcagt cggtaagaat gaatcattcc tatcgcagaa aagaatgatg
      781 tgatcagaca ccagtgaccg gccttaatga actgtctttc cagaacagcc acgcagattg
      841 ccgcccagaa cattgaaaca agcagaaacc cctgattgag tgccagcata gcatggagcg
      901 gataggaaaa aggaggaagg agcatcatat caggagaaag atctgacatg acaatagcat
      961 acttgtttgc tatgttttct tttgcaagta tctcgcccag tttcccgttt gcaaaattga
     1021 atatgctttg gacaagcagg actccccatc ctgcaagtgc agggaaaatt ccgagcacaa
     1081 tggcgggtgc gtgtttgatc ggtgaacttt caaatgcctg tgcaccgata atgatcccta
     1141 tccagagaat aattgccata cctgcttcaa ttggaactac tgctgaaata agactcataa
     1201 atccgaaaaa gccgatcagt gacataactg caccgttcag aac
//
LOCUS       DAOQGJ010001562         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_90980_length_1242_cov_4.043808, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001562 DAOQGJ010000000
VERSION     DAOQGJ010001562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90980_length_1242_cov_4.043808"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PK683_19640"
     CDS             <1..683
                     /locus_tag="PK683_19640"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH10721.1"
                     /translation="HNLEIFPYYSAMSEVGGDFYGVSIVSQNHYRIFLADVTGHGIQA
                     ALITMAVKGIYDDIRKFELSPSEILDIFNDEFFEKYQSLNSLLTCIFVDIYPNERKIQ
                     YASAGHPPCILLSEGEIHLLEKTGRMIGIKKANMYRTVEMPFSENDRILLFSDGIYEQ
                     FDSEGKEFGEDRLRELIQKTSNLRLKSSIEAVISDLNLFLGRQEKQDDITILGIELKK
                     SEQDRQTD"
     gene            812..973
                     /locus_tag="PK683_19645"
     CDS             812..973
                     /locus_tag="PK683_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10722.1"
                     /translation="MKFSEALEVSEREGIAIAEAGKMLDIGETCTACRTDFKEYCQKA
                     GVRNWAEAC"
     gene            complement(976..>1242)
                     /locus_tag="PK683_19650"
     CDS             complement(976..>1242)
                     /locus_tag="PK683_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10723.1"
                     /translation="NAKELDDLKAKNEKELQDLKDKNNAMKNEIKDNASGQKDEIKNK
                     ASGDVNDVKKASGQADEIKKAGTNDSGKSALDELKNKNKGMTKP"
BASE COUNT          368 a          237 c          258 g          379 t
ORIGIN      
        1 ttcacaatct ggaaatattc ccctactatt ccgcgatgtc tgaagtagga ggagattttt
       61 acggagtcag cattgtatcc caaaatcatt atcgtatatt tcttgcagac gtgaccggtc
      121 acggaattca ggcagcgctc atcacaatgg cagtgaaagg aatttatgac gatatcagaa
      181 agtttgaact ttcaccctcg gagattctgg acatattcaa tgatgaattt ttcgagaaat
      241 atcagtcact gaattctctg ctgacatgca ttttcgtaga catttatcct aatgaaagaa
      301 agattcaata tgcttctgca ggccatcccc cctgcatcct tctttcggaa ggggaaattc
      361 atcttctgga aaaaacaggc aggatgatcg gaatcaaaaa agcaaatatg tacagaactg
      421 tagaaatgcc tttttcggaa aatgacagga ttctcctttt ttctgacggg atttatgaac
      481 agtttgactc tgaaggaaag gaattcggag aagacaggct cagagaactg atccaaaaaa
      541 catccaatct tcggctgaag agctccattg aggctgtaat ttcagacttg aaccttttcc
      601 ttggaaggca ggaaaaacag gacgatatca caattcttgg gattgagctt aaaaaatcgg
      661 aacaggacag gcaaactgat taagagactc agaaaaaatg ctggaaggaa aatcataatt
      721 cctgtatttt ggattcgtac aagaaattct cttgacaatt taagaatgag tctcaaaatt
      781 agatcatatg gaaaaatgtc attgcagcag agtgaaattc agtgaagcgt tggaagtctc
      841 ggaaagagaa ggaattgcaa tcgcagaggc aggaaaaatg cttgatattg gtgaaacctg
      901 caccgcctgc agaacagatt tcaaagaata ctgtcaaaaa gcaggcgtga ggaactgggc
      961 agaagcctgt tagctttagg gctttgtcat tcctttattt ttgtttttaa gttcatccag
     1021 tgcacttttg cctgagtcat tcgttccggc tttttttatc tcgtcagcct gaccagatgc
     1081 ttttttaaca tcatttacat cgccggaggc tttattttta atttcatcct tctgcccgga
     1141 tgcattgtct ttaatctcat ttttcattgc attgtttttg tctttcagat cctgaagttc
     1201 tttttcgttt ttggccttca ggtcatccag ttcctttgca tt
//
LOCUS       DAOQGJ010001563         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91023_length_1242_cov_2.540859, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001563 DAOQGJ010000000
VERSION     DAOQGJ010001563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91023_length_1242_cov_2.540859"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(555..>1242)
                     /locus_tag="PK683_19655"
     CDS             complement(555..>1242)
                     /locus_tag="PK683_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10724.1"
                     /translation="KLTTEYTLHNWLTDKKNPEIYRNYLFGKIVNPFINEEDTTIVDV
                     YTSAKYSFEDISNTFPKTECRGLASAYLYETLSISFASMDLWKQTILSILIEKNGDVE
                     YGTVLNIFSEDSFYNEDIKSYIERIGEIVLETTNIPPDKKKIHLADHHGKAELQAFCN
                     KIKNSPYVIEMRSTNWGGNKFIRKIQKDGVIEVVLIKTDKRYALWIQTTGKNYRQTEQ
                     ISEILDDDYS"
BASE COUNT          352 a          217 c          194 g          479 t
ORIGIN      
        1 tatggggtta tctttacggc cgttccgcag acaagaaaaa ggagatgaac agacgcggat
       61 attttcttct ttttccttaa ttacaggttt gtcaagaaaa taatattcat ggaaagaatt
      121 ttctagactg tcttcggctc aattccattc agtatccagt caggattaat tccaaattta
      181 tatgccatat aataaagcaa aactttcggg atgtttttct ctgtgcatac tcactgaaac
      241 agtctctgaa atcattcagc catttaatta tattttcctc ggatatatca aaaagatttt
      301 ttatatctga caaggttttt aattccctgg acatggagtc tctaagctgg tttaagttta
      361 atctgttgtc ttttttcata acatcattcc gcaaattcct ggctgccagg gttacctatt
      421 tttttcctga tttcattata tttcaataaa agatcttttt cattcagaat cctgcagcct
      481 tcttcaatcc aatgcagtga gtctctgtag atttttgcga gaacaggatt ttcctgtata
      541 agggagaaag gagtttatga ataatcatca tctagaattt cactgatttg ctctgtttgt
      601 ctgtagtttt taccggttgt ttgtatccat agggcatacc ttttatcagt ttttattaag
      661 actacttcaa tgactccatc tttttgaatt tttctgataa atttatttcc cccccagttt
      721 gtagagcgca tctcaattac atatggacta ttttttattt tattacaaaa agcctgaagt
      781 tctgcctttc catgatgatc agccaaatga atttttttct tatctggagg aatatttgta
      841 gtttctaaaa caatttcgcc aattctctca atataagatt ttatatcctc attgtaaaat
      901 gaatcttctg aaaatatatt taaaactgtt ccgtattcaa catctccatt tttttctata
      961 agtatagaaa gtatagtctg tttccataaa tccattgatg caaaactgat acttagagtt
     1021 tcatataaat acgcagatgc cagtccccga cactccgttt ttggaaaagt atttgaaatg
     1081 tcctcgaaag agtatttggc tgatgtatat acatcgacta ttgttgtgtc ttcttcgtta
     1141 ataaaaggat ttactatttt cccaaaaagg taattgcgat atatttcagg gtttttttta
     1201 tccgtcagcc aattgtgtaa tgtgtattcc gtggttagtt tg
//
LOCUS       DAOQGJ010001564         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91069_length_1242_cov_1.900590, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001564 DAOQGJ010000000
VERSION     DAOQGJ010001564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91069_length_1242_cov_1.900590"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PK683_19660"
     CDS             complement(<1..531)
                     /locus_tag="PK683_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH10725.1"
                     /translation="MQESEIRTIDCRYIKENLAASFLIQEGNECVFVETNTVYAVPYL
                     LKTLEEAGRKPEDVKYIIVTHVHLDHAGGTSELLKHCPNATVLAHPKTASHLAAPERL
                     VQSSITVYGEEKFRELYGNIEPVPEKRIRIMKDGEELVWGKRKLRFIYTRGHANHHFV
                     IHDSKSNSVFTGDSFGI"
     gene            complement(508..987)
                     /locus_tag="PK683_19665"
                     /pseudo
     CDS             complement(508..987)
                     /locus_tag="PK683_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            complement(981..>1242)
                     /locus_tag="PK683_19670"
     CDS             complement(981..>1242)
                     /locus_tag="PK683_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10726.1"
                     /translation="YGLGLHIFKRGKNVEAYHHNGVGDNLFTHMQILPLKSEIVFFHF
                     TLPDMNIKVRNILSEKLVKLFYKLRILEEKIIKFRKKNRIKS"
BASE COUNT          298 a          298 c          239 g          407 t
ORIGIN      
        1 aatcccgaat gagtctcccg tgaagacaga attggatttt gaatcatgaa tcacaaaatg
       61 atgatttgca tgtcctctgg tgtagatgaa tctgagcttt ctttttcccc agacaagttc
      121 ctcaccgtcc ttcatgattc tgattctttt ttccggaaca ggttctatat tcccgtacag
      181 ttctctgaat ttttcttctc cgtatacagt gatggaactc tgcacaagtc tttccggggc
      241 ggccaggtga gatgcagtct taggatgagc gaggacagtc gcattcggac agtgtttcag
      301 cagttcggat gtgcctcccg catgatcgag atgcacatga gtcacgatga tatatttcac
      361 atcctcaggc tttcttccgg cttcctcgag agtcttcaga agatacggaa ctgcatagac
      421 agtgtttgtt tccacaaaca cacattcatt tccttcctga atgagaaatg aggccgccag
      481 gttttctttt atgtacctgc agtctattgt tcttatttct gattcctgca gataattttg
      541 aatttccctc tgaaatgcag gaaacatctt ttcgtacaca tctttcttga atgatactat
      601 attctttaca acatcattat aatccatgaa acggacgctc aggaattcct gttcatgcac
      661 atgcagattg cagtcctccg cctttccgtt ccagaaatac aggaaccatt tctgagtctg
      721 tccctggtat tttttcagat gggccgggat gtcttccggg aaatcatagt tcagccattc
      781 cggaaactcg aacacaggtt ctgcatcatt gatccctgtt tcttcataca gttcccttct
      841 gcccgcaaat acaggatctt ccccttcatc tatcccgccc tgagggaact gccaggcgcc
      901 cggatactgg accctttctc cgacaagaac ctttccttcc ctgttgaata ctacaactcc
      961 ggtgttcttt ctgtagggtt tcatgatttt atcctgtttt tctttctgaa tttaatgatt
     1021 ttttcttcaa gaattctgag cttgtagaaa agtttcacca gtttctcgct gagaatgttc
     1081 cttactttga tattcatatc cggaagagtg aaatgaaaaa atacgatctc gcttttcaga
     1141 ggaagaatct gcatatgagt gaaaagattg tctccgactc cgttatgatg ataggcttcc
     1201 acattctttc ccctcttgaa tatatgcagt cccagtccgt ag
//
LOCUS       DAOQGJ010001565         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91151_length_1241_cov_2.994098, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001565 DAOQGJ010000000
VERSION     DAOQGJ010001565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91151_length_1241_cov_2.994098"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PK683_19675"
     CDS             complement(<1..271)
                     /locus_tag="PK683_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10727.1"
                     /translation="MIQFPGLDTMESLHSGRHSLVFRVQRRGESFILKYLNYDYPSTD
                     IYRNFVREFETLCQVRMPGVVEAVSLEKYRNSLYILFRDRGGRALS"
     gene            complement(423..>1241)
                     /locus_tag="PK683_19680"
     CDS             complement(423..>1241)
                     /locus_tag="PK683_19680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH10728.1"
                     /translation="MLSAQTAVSLENSILYSSMEKKVEERTRSLEYANAELEKLAQSR
                     LRLSAIGEMVSGLVHDIKNPIGTVKAFAEMIKNPETDSETRSEYADLILRETDRLGDI
                     AYEILDFSKGKISLNLENHRVDGFLDELYQFLKIDFEYAGISFNVECNYEGELSFDRD
                     RLRRVIVNLANNSREAMSAWKGERHFSISAEKMKDSAIFRVEDTGPGIPESIADRLFE
                     AFATEGKQKGTGLGLYMSRMIAEQHKGSLTYSPYEGSGAVFIIKIPIQDQEKKL"
BASE COUNT          312 a          329 c          224 g          376 t
ORIGIN      
        1 acgacagcgc cctgccccct ctgtctctga aaagaatata cagactgttc ctgtattttt
       61 ccagactcac tgcttccacg actccaggca tacgcacctg gcaaagtgtc tcgaattccc
      121 ttacaaaatt tctgtatata tctgtggacg gataatcata attcagatat ttcagtatga
      181 agctctcgcc tcgcctctgc accctgaaca ctagagaatg ccttccggaa tgcagacttt
      241 ccatcgtatc cagtcctgga aattgaatca cctgacagta cctcccggaa aaattgataa
      301 ttggttaaaa tgcatcaaag ccgtctgcaa agcattgtac tttacagttt tcagtaaagt
      361 atgttttgtc attcccggat ctgcactgaa agaaaagatt tcactggaca gtaccgcttc
      421 catcataatt ttttctcctg atcctgtatc ggaattttaa tgataaaaac agcacctgag
      481 ccttcatacg gactgtaggt cagactccct ttatgctgct ctgcaatcat tctgctcata
      541 tacagtccga ggccggttcc cttctgtttt ccctccgtgg caaatgcctc gaaaagtctg
      601 tcagcgatgc tttcaggaat gcccggtccc gtatcctcta ccctgaaaat ggcagaatct
      661 ttcatctttt ccgctgaaat tgaaaaatgc ctctcacctt tccaggcaga catcgcctct
      721 cttgagttgt tcgcaagatt cactatgact ctccggagtc tgtcccggtc aaaactgagc
      781 tcaccttcat aattacattc tacattaaat gagataccgg catactcaaa gtctattttc
      841 agaaactgat acagctcatc cagaaatccg tctaccctgt gattttccag attcaggctt
      901 attttaccct tgctgaaatc cagtatctcg tatgcgatat ctcccagtcg atcagtttcc
      961 ctgagaatca gatctgcata ctctgaacgg gtttctgaat cagtctccgg attctttatc
     1021 atttctgcaa acgctttcac tgttccgatc ggattcttga tgtcgtgcac aagacctgat
     1081 accatctcgc ctattgcaga aagcctgagt cttgactgag caagtttttc cagttccgca
     1141 tttgcatatt ccaggcttcg tgtacgttcc tccactttct tttccatact ggaataaagg
     1201 atggaattct ccaggctgac cgcggtctgt gcagaaagca t
//
LOCUS       DAOQGJ010001566         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91280_length_1240_cov_3.468354, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001566 DAOQGJ010000000
VERSION     DAOQGJ010001566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91280_length_1240_cov_3.468354"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PK683_19685"
     CDS             <1..239
                     /locus_tag="PK683_19685"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769171.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNH10729.1"
                     /translation="EYGDLTRGPRIIDAGTKARMKEVLGEIQKANGAKFAKEWVAETK
                     AGYPEFQKLRDKGAAHPIEAVGKKLRGMMKWLAK"
     gene            240..1142
                     /locus_tag="PK683_19690"
     CDS             240..1142
                     /locus_tag="PK683_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNH10730.1"
                     /translation="MEKAYLGIDAGGTGIKAGLIFRSGEIIFESNTPTGKNWGNEDFL
                     NAVSSLCEKAFSVQKPEAIGIGTPGPIDIENGIILHSANMHGLKNVPLTESLKKKFSV
                     PVCFNNDANCAALGEYHFGAGTGSKNLLVITLGTGLGAGWVLDGKIYNGFQGNGMELG
                     HTTVEPDSKALCGCGQYGCIESYFSASGLLNRYEEMTGRRLANALEFFSLVQSGDEKA
                     GDVLEKALGYFALTLRTAVHTVNPDRIVLVGGLAKSSDLYLEKLKKKVSQIVFPVLWN
                     NLRLETGKNVAGTLGAGSLCFASR"
BASE COUNT          390 a          241 c          303 g          306 t
ORIGIN      
        1 ctgagtacgg agatctgaca agaggtccaa gaatcattga tgcaggaaca aaagcgagaa
       61 tgaaagaagt tctcggtgaa atccagaagg ccaacggtgc aaaattcgca aaagaatggg
      121 tggctgaaac aaaagcagga tatcctgaat tccaaaaact ccgtgacaaa ggagctgcac
      181 atccgattga agcagtcggt aaaaaacttc gcggaatgat gaagtggctg gctaaataat
      241 tggaaaaagc ttatctgggg attgatgccg gcggaaccgg catcaaggct ggtctgatct
      301 ttagatcagg tgaaatcatt tttgaatcaa acactcccac tggcaaaaac tggggaaatg
      361 aagatttttt aaatgcagtc agttctctct gtgaaaaagc tttttcagtt caaaaaccgg
      421 aagcaattgg aatcggcact cccggaccga ttgatattga aaacggaatc attcttcatt
      481 cagccaatat gcatggactg aaaaatgtcc ctctcactga atcactgaaa aaaaaattct
      541 ctgtcccagt atgttttaac aatgatgcca actgtgctgc gctgggagaa taccatttcg
      601 gtgcgggcac cggttcaaaa aatcttctgg tcattacgct tggtactggg cttggagcgg
      661 gctgggttct ggacggaaaa atctacaacg gctttcaggg aaacggaatg gagcttggtc
      721 atactacagt ggaaccggac agcaaggctc tctgcggatg cggtcagtac ggatgcatag
      781 aaagttattt cagtgcaagc ggtctcttga acaggtacga agaaatgaca ggaagaagac
      841 tggcaaatgc ccttgagttt ttttctcttg ttcagtcagg tgatgaaaaa gccggcgatg
      901 tcctggaaaa agcactcgga tattttgcac tgactctcag aacagcggtg catacagtaa
      961 atcctgaccg aattgttctg gtaggaggac ttgcaaaatc ttccgattta tacttggaaa
     1021 aactcaaaaa aaaggtgtct caaatcgtat tccctgtact ctggaataac ctcagactgg
     1081 agaccggaaa aaatgttgca ggcactcttg gagctggctc gttatgtttt gcctctcgat
     1141 aaatgaattg ctgcataaaa ctgtaattct atgtttacat tggaaaaagt aactggagct
     1201 gaaaaaacgg gatttttaag ggcgaagccc ttaaaactgc
//
LOCUS       DAOQGJ010001567         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91322_length_1240_cov_2.615190, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001567 DAOQGJ010000000
VERSION     DAOQGJ010001567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91322_length_1240_cov_2.615190"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PK683_19695"
     CDS             <1..405
                     /locus_tag="PK683_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10731.1"
                     /translation="KYRIALEKILPEYYGEYALAAFTFSDISDQRFKFRRIYKYYYNK
                     TKKKELLKEGQDPAEILFSIREDGSFYIWETEKNGNSVRLQLHDRNGIHIQNRKLKFF
                     QPRNLWRETYAGKSGIYSVRIESGHMELYEWK"
     gene            396..>1240
                     /locus_tag="PK683_19700"
     CDS             396..>1240
                     /locus_tag="PK683_19700"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNH10732.1"
                     /translation="MEMKGRNIFLSEENRRLDLYTMEHKKIPGTILMGMAGASVWQRI
                     RKITEGYKIIIFSGSGNNGGDGFVLAHLAAEEGRDVSLFFREGKTGSEEYMFYRELAL
                     SNPMIKVFPFSESFERPEFKGEKTAILDCILGTGFRNPLSNELLDVFSRIGRLREYVG
                     EMKVIGIDTSSGYDGGRTFPLPPDLICEIGVRKLENIYASVLCRKKHLVTIGFPEKEF
                     VQADSEKKKIFEGTFLKKKEILKISRRKNNSDKYRNGAASFIGGSSGMEGAVFLSEKA
                     FRIMGG"
BASE COUNT          425 a          223 c          296 g          296 t
ORIGIN      
        1 aaatacagaa ttgctctcga aaaaattctt ccggaatact acggagagta tgctctggct
       61 gcatttacat tctccgatat atccgatcag aggttcaagt tcagaagaat ttataagtat
      121 tattataata aaacgaaaaa gaaggaactt ctgaaagaag gtcaggatcc tgcggaaatt
      181 ctgttcagca tcagagagga cggcagcttt tacatctggg aaacagagaa gaacggaaat
      241 tcagtcagac tgcagcttca tgacagaaac ggcattcata ttcagaacag aaaactgaaa
      301 ttctttcagc cgagaaatct ctggagagag acgtatgcag gaaaatcagg catttattcc
      361 gtgcggattg aatccggtca tatggaactt tacgaatgga aatgaaagga aggaacattt
      421 ttctttcgga agaaaacaga agactggatc tctatacgat ggagcataaa aaaattcctg
      481 gaacaatcct gatggggatg gcaggcgcat ccgtctggca gagaataaga aagatcaccg
      541 agggatataa aatcattata ttttcaggat cagggaacaa cggcggagac ggattcgtgc
      601 ttgctcatct ggctgcggaa gaaggaagag atgtttctct tttcttcagg gaaggaaaga
      661 cgggaagcga ggaatatatg ttctacaggg aactggcgct ttcaaatccg atgataaaag
      721 tctttccttt ttcagaatcc ttcgaaagac ctgaattcaa aggagaaaag acagctatcc
      781 tcgactgcat tctgggaacg ggattccgaa accctttaag caatgaactt ctggatgtgt
      841 tcagcagaat cggcagactg agagaatatg tcggtgagat gaaagtcatt ggaatagaca
      901 cgtcctccgg atatgacggc ggcagaacat ttccgcttcc tcctgatctg atctgtgaaa
      961 taggtgtaag aaaactcgaa aacatatatg catcagttct ctgcagaaaa aaacatcttg
     1021 tcacgatcgg attccccgaa aaagaattcg tacaggctga ctctgaaaag aaaaaaatct
     1081 ttgaaggaac ctttcttaag aaaaaggaaa ttcttaaaat cagcagaaga aaaaataatt
     1141 cggacaagta cagaaacgga gcggcaagtt tcatcggcgg gtcatcagga atggaaggag
     1201 cagtatttct ttccgaaaag gcttttcgga tcatgggagg
//
LOCUS       DAOQGJ010001568         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91340_length_1240_cov_2.372996, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001568 DAOQGJ010000000
VERSION     DAOQGJ010001568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91340_length_1240_cov_2.372996"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PK683_19705"
     CDS             <1..542
                     /locus_tag="PK683_19705"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH10733.1"
                     /translation="LGLTPAHTAADSGQWNLLKLLLNSGADPDSTDAKGNTVLHKAAG
                     IKGNLNSVRFILQKNADKNIKNNDEETALHISVLRAADLTITQHLISEGADINMKDSD
                     GDTILHMLCIQKTNTEMINTVISGKAKVNSVNQTGKTPLHYAVQFKRTETIEILLKNG
                     ADPYLRDLNGHTPEDLKPK"
     gene            complement(496..>1240)
                     /locus_tag="PK683_19710"
     CDS             complement(496..>1240)
                     /locus_tag="PK683_19710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNH10734.1"
                     /translation="AMTVAREIKQRILEETGLTSSVGISYGKFLAKIASDYNKPNGFK
                     VILPEEAVDFISALPIGKFYGIGKVTEKKMLSLGIKNGADLRERPLEFLQRNFGKAGG
                     YYYRIVRGLDTSEVCSYHERKSLGTEDTFEKDISTEEEAAKELIRLSDRLFERADKDG
                     VYGRTITLKIKYSDFTLVTRSKSRKEFYSDKEEALRESLGLFRENFSGKKIRLLGISF
                     SNLGKEETENWEMLFTSVSDLQEYVHSDL"
BASE COUNT          366 a          285 c          241 g          348 t
ORIGIN      
        1 atctaggtct gactcctgcc catacggccg ccgactcagg tcaatggaat ctgctgaaac
       61 ttctgctgaa ttcaggagct gatccggaca gtaccgatgc aaaaggaaat actgtacttc
      121 ataaagctgc aggcattaaa ggaaatctga attcagtaag gtttattctg cagaaaaatg
      181 cagataaaaa cataaaaaat aatgatgagg aaactgcact tcacatatct gtcctgaggg
      241 ctgcagattt gacgataact cagcatctca tttcagaagg tgccgatata aatatgaaag
      301 acagtgacgg cgatacaatt cttcatatgc tttgtatcca gaaaacaaat accgaaatga
      361 taaacacagt catatccgga aaagcaaaag taaattctgt aaatcagaca ggcaaaacac
      421 ccctccacta tgcagttcag ttcaaaagaa cagagacaat agagattcta ttaaagaatg
      481 gagcagatcc gtatctcaga gatctgaatg gacatactcc tgaagatctg aaaccgaagt
      541 gaaaagcatc tcccagttct cggtctcttc ttttcccaga ttggaaaatg aaattccaag
      601 aagacggatt ttctttccgg aaaaattttc ccggaaaagt cccagtgact ctctgagtgc
      661 ctcttcctta tctgaataga actcctttct gcttttggat cttgtaacca aagtgaagtc
      721 actgtatttt attttcagcg taatcgtcct tccataaacc ccgtctttgt ctgcccgttc
      781 aaacagtctg tccgaaagac gaatcagttc ctttgcggcc tcctcttcag tggagatgtc
      841 tttttcaaat gtgtcctctg tgccgagact ttttcgttca tgataggaac agacttccga
      901 tgtatccagc cctctcacta tcctgtaata ataaccccct gcctttccga agtttctctg
      961 aaggaactcc aatggtcttt cccgaaggtc agctccgttt ttgattccga gagagagcat
     1021 tttcttttca gtgacttttc caatcccgta aaactttccg ataggaaggg cacttatgaa
     1081 atccactgcc tcttcaggaa ggatgacctt gaagccgttc ggcttattgt aatcggatgc
     1141 aatttttgca agaaatttcc cataggaaat tccgacagat gaggtgaggc ctgtttcttc
     1201 cagaatcctc tgcttgatct ctcgggcaac agtcatagca
//
LOCUS       DAOQGJ010001569         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91407_length_1239_cov_4.554054, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001569 DAOQGJ010000000
VERSION     DAOQGJ010001569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91407_length_1239_cov_4.554054"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PK683_19715"
     CDS             complement(<1..466)
                     /locus_tag="PK683_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002741309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH10735.1"
                     /translation="MNTPVKKASLKELFGWCMFDFANSSYTTVIITVVFGDIFSTILV
                     PSSGDPENPYKYGNMLWAVALAISYILVVISGPFFGAVMDYSARKKQFLFYSYLLCIF
                     STALLFFAGNTGEYWLAFFLIIISNFAFASGENFAASFLPFLGPKDELGKISG"
     gene            727..>1239
                     /locus_tag="PK683_19720"
     CDS             727..>1239
                     /locus_tag="PK683_19720"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH10736.1"
                     /translation="MKKISFIILLLFAVTLKADSTDDALNKAVCDFDLDRLKSIVSAG
                     ITPKQKDNALNLAAYCYGGFPEGANYLLDMGADPDNFEKGPYSYSTLMWALRSTSDKT
                     SGMKPVLDRILEKVKDVKFAHVDTKDTALHWAAANGDYEVIKILLKKGADPKAKTSGY
                     FGDPPSTPADY"
BASE COUNT          408 a          246 c          265 g          320 t
ORIGIN      
        1 ttcctgaaat tttacccagt tcatccttcg gtcctaaaaa aggaagaaag ctggcagcaa
       61 agttttcccc tgaggcgaat gcaaaattgg aaatgatgat caggaaaaag gcaagccaat
      121 actctccagt gtttcctgcg aagaacagaa gcgctgtgct gaaaatacaa agcagataag
      181 aataaaaaag gaactgtttc tttctggcgg aatagtccat cactgcacca aaaaaaggtc
      241 ctgaaatcac aactagaata taggaaatgg caagagcaac tgcccaaagc atatttccgt
      301 acttgtaggg attttccgga tcgcctgagg aaggcacaag tattgtactg aagatatcac
      361 cgaacactac tgtgataatg actgtcgtgt aggaggaatt ggcaaagtca aacatacacc
      421 atccgaagag ttccttcagg ctggcttttt ttacgggtgt attcataagt gcgtctcgag
      481 tgctcccaag ttatagaatg ctctgcagaa tggaagtgtt tttgcaggga taaatataaa
      541 aatctcagtc cttgaatcga gactcattaa cggtacacag atatgcaatt tctgcttttt
      601 tttatctgaa tcaatgagag ccgtgtctaa taccaagttg aaagctcttt aaatggaaag
      661 gtttaaaggg gatcaattta ctctttaaat atgtccgaat ttttaattcg ggtcaggaga
      721 atatgaatga agaaaatcag ttttataatc cttctgcttt ttgcagtaac cctcaaagca
      781 gacagtacag atgatgcgct gaacaaagct gtctgtgatt tcgatttgga cagactcaaa
      841 tccatcgtat cagccggaat cacacctaaa cagaaagaca acgcactgaa tcttgctgcc
      901 tactgttatg gaggatttcc cgaaggggca aattaccttc tggatatggg agctgatcct
      961 gataattttg aaaaaggacc gtacagttat tccaccttaa tgtgggcgct tcgcagtacc
     1021 agcgacaaaa catcaggaat gaagcctgta ttggacagaa ttctggaaaa ggttaaagat
     1081 gtaaaatttg cccatgtaga tacaaaagat acggcactcc actgggctgc agcaaacgga
     1141 gactatgaag tcataaaaat ccttctaaaa aagggagcag atcctaaagc aaaaacatcc
     1201 ggttattttg gagaccctcc ctctaccccg gcagactat
//
LOCUS       DAOQGJ010001570         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91424_length_1239_cov_3.493243, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001570 DAOQGJ010000000
VERSION     DAOQGJ010001570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91424_length_1239_cov_3.493243"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1239
                     /locus_tag="PK683_19725"
     CDS             <1..>1239
                     /locus_tag="PK683_19725"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10737.1"
                     /translation="ASGRLRTASIFGKSGAGKSALVNEIVRPINEKRGFFVFGKYDQF
                     KKDVPYSAFIKIFTDLIRLILSERQETVSFFRKSVLNAVGLNGKIMTDLIPELALLIG
                     EQPETEILPPEESLNRFELVLQAFINSICTAEHPVAVFLDDMQWADLPSIKLLESIIF
                     NFDSKYLFIMISYRDNEVLPADALYLLLNELNKTGNFHRQIELKPLDIEDIALLVRDV
                     ISDSEQVASEIAEVLLDRTQGNPFFIRELINTFYERRLVFLKEGKWKVDISGIRSSEI
                     SANAAGLMVEKIAGMPDETVSFLKYAACIGDSFQKQIIYMLTDSSEENIDRILTEAAN
                     EGFFIISGDSVQFSHDKIREAAYSLISDTDRSYIHYKIGNIYYNIYNENELSENVFII
                     VNQLNKGLENIRDDAERQRIL"
BASE COUNT          365 a          237 c          277 g          360 t
ORIGIN      
        1 gcctccggcc gtcttcgaac agcttccata ttcggcaaat cgggtgcagg gaagtctgca
       61 cttgtaaatg agattgtccg gccgatcaat gaaaaaagag gattttttgt tttcggaaaa
      121 tacgaccagt tcaagaagga cgtaccgtac agtgctttta taaaaatttt tacagatctt
      181 atcaggctga ttctttcaga aaggcaggaa acagtctcct ttttcaggaa atccgttctg
      241 aatgcagtag gactgaacgg aaaaattatg actgatctga ttccggaact ggcacttctc
      301 ataggcgagc agccggaaac ggagatcctt cccccggaag aatctttgaa ccgtttcgaa
      361 ctggtgctgc aggccttcat aaattcaata tgcactgcag aacatccggt tgccgtgttt
      421 ctggatgata tgcagtgggc agatcttccc agcataaaac ttttggagag catcatattc
      481 aatttcgatt cgaaatattt atttataatg atctcttaca gagataacga agttctccct
      541 gcagatgctc tttacctgct tttgaacgaa ctgaataaaa ctggaaattt tcacaggcag
      601 atagaactga agccgctgga cattgaagac attgctcttc ttgtcaggga tgtgatctct
      661 gattccgagc aggtcgcttc tgaaattgca gaagtccttc tggacaggac ccaggggaat
      721 ccgtttttta tcagggagct gatcaatact ttttatgaaa ggagactcgt attcctgaaa
      781 gaagggaaat ggaaggttga tatttccggg ataagatctt cggagatttc tgcgaatgcc
      841 gcaggactca tggttgaaaa aattgccggt atgccggacg aaacagtcag ttttctcaaa
      901 tacgctgcct gtatcggaga ctcttttcag aaacagatta tttatatgct cactgattcc
      961 tctgaggaaa acattgacag gattctgacc gaggcggcaa atgaaggttt tttcatcatc
     1021 agcggagatt ctgtgcagtt ttctcatgat aagatccgtg aagcggcata ttcactgatt
     1081 tcggatactg accgttcata tatacattat aaaataggta atatatatta taatatatac
     1141 aatgaaaatg aactttctga gaatgtattt atcattgtca atcagctgaa caagggtctt
     1201 gaaaatatcc gtgatgatgc tgaaagacag agaattttg
//
LOCUS       DAOQGJ010001571         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91451_length_1239_cov_2.785473, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001571 DAOQGJ010000000
VERSION     DAOQGJ010001571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91451_length_1239_cov_2.785473"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..>1239
                     /locus_tag="PK683_19730"
     CDS             343..>1239
                     /locus_tag="PK683_19730"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH10738.1"
                     /translation="MIFRFLILLLFVLPLKSDERISLKQICEGESCRPRVWEIREGFD
                     PASAFSESSDSSWKKVDSFPIWADKFFPPKNDFAVYTYRTVFDAPNEFLTGDRQPGIK
                     LGEAGEVFSIYINGNLIASEGKSENGKTVHHRTVRNKVWQINPKYLRLKNNMLVLKIE
                     GDPRYDHTGLYFTQGYEIDFLDELEFEKRDILGISFMGIYFVVGLYHLLLFSKRRNEL
                     YNLYFGVFSLFLPVYIFTRSNIVFEFPVDSILIQRVELIVLYPLPMLITVFAEQLFFG
                     KVSKFTWKMLFLSMALALPNLFV"
BASE COUNT          358 a          239 c          249 g          393 t
ORIGIN      
        1 ccggaaaaaa taaaaaaaat cagactttta agccgaggat cttgctgttt ttactgatcc
       61 atcgcaaact tttaaacagc tggatttccc taatcttttc taaaaattac cgcttccaga
      121 cagcatattg tatttgcgca aggtcagaat gcaagtcgaa atctgagtca gagagttcag
      181 agaactgttt gtatggactg gctttaagaa taaatttact gccttcggga tgcagaatca
      241 ttatcctccg atgtacttct tattttgaga agaaagagaa aattagattt tttatgattt
      301 gacagtcaaa ttccttatga tccaatgtct aggcagttta ctatgatatt ccgattcctt
      361 attctgcttt tgtttgttct ccccctgaaa tctgatgaaa gaatcagtct gaagcagatc
      421 tgcgagggcg aatcctgtcg gcccagggta tgggaaattc gcgagggctt tgacccagca
      481 tctgcatttt ctgaatcatc cgacagttcc tggaaaaagg tggacagttt tccgatctgg
      541 gcagacaaat ttttcccacc aaaaaatgac tttgctgtgt atacgtacag gacagtattt
      601 gatgctccga atgaatttct gacaggagac agacagcctg gaatcaagct gggagaagca
      661 ggagaagttt tttccattta catcaacgga aatctgattg catctgaagg aaaatctgag
      721 aacggaaaaa ccgtccatca caggaccgtc cgaaacaagg tctggcagat caatccgaaa
      781 tacctccgtc tgaagaacaa tatgcttgtc ttgaaaatag aaggcgaccc cagatacgat
      841 catacaggtc tttattttac gcagggatat gaaattgatt ttctagatga acttgaattt
      901 gaaaaaagag acatcctcgg aatcagtttt atggggatat attttgttgt aggcctgtat
      961 catcttcttc ttttcagcaa aaggagaaat gaactttaca atctgtactt cggagtgttc
     1021 agtcttttcc tgccagttta catatttacc agatcgaata tcgtatttga atttcctgtg
     1081 gattccattc tgattcagag agtagaactg attgtgcttt atcctcttcc tatgctcatt
     1141 actgtttttg ctgaacagct tttctttgga aaagtttcca aatttacatg gaaaatgctt
     1201 tttctttcaa tggcgctggc tttgcctaac ctctttgtt
//
LOCUS       DAOQGJ010001572         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91542_length_1238_cov_3.903635, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001572 DAOQGJ010000000
VERSION     DAOQGJ010001572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91542_length_1238_cov_3.903635"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1238)
                     /locus_tag="PK683_19735"
     CDS             complement(<1..>1238)
                     /locus_tag="PK683_19735"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH10739.1"
                     /translation="IFLLAATLRYMQYGAVLFSGIISTVLILGALRNFPVFSGYSLNE
                     KLLILQTFIGVNSIAMLLLSVLLYQQQKAQESLLELNRDLDLKVMERTEDLQKSLAEI
                     QKLKIMQDGDYFLTSLLIQPFIVNLVQSEKAEAEFLISQKKKFEFHNKKYEIGGDMCH
                     AQNLVLEGKKYILFINSDAMGKSLQGAGGAIVFGSVLFSIIGRTRDRNISPEKWLSDA
                     FQELNDVFLSFDGSMLVSVILCLLDEDTGVLYYINAEHPSSVIYRDNICEFIDKENRL
                     FKLGSCFNTLKKVSVQTVSLNRNDTVILGSDGRDDIDLQENAHKIRKNFDETLFLRTV
                     EEAEGDLKKIFRLTEEKGMITDDYSLMKIKYKNEIHPRNDIRKSALEHYNRKNFKEAA
                     VYAEKYAELFPGDTDIFYIL"
BASE COUNT          356 a          270 c          229 g          383 t
ORIGIN      
        1 caaggatata aaatatatca gtgtctccgg gaaaaagttc agcatatttt tctgcgtaga
       61 cagcggcctc cttgaaattt tttctgttgt agtgctcaag tgcagacttc ctgatgtcgt
      121 ttctgggatg aatctcattt ttatatttta ttttcattaa agaatagtca tccgtaatca
      181 ttcctttttc ttctgtcagt ctgaaaattt tcttcagatc tccttccgct tcttctactg
      241 ttcttagaaa cagagtctcg tcaaaatttt tcctgatctt atgagcgttc tcctgaagat
      301 caatgtcatc tcttccgtct gagccgagga tcaccgtatc atttctgttc agagaaacag
      361 tctgaacaga aacctttttg agtgtattaa agcagcttcc cagcttgaaa agcctgttct
      421 ctttatcgat aaattcgcag atattgtctc tgtagattac actcgaagga tgctctgcat
      481 tgatataata taaaactcca gtatcttcat ccagaagaca gagaatcacc gatacaagca
      541 tacttccgtc aaagctgaga aacacatcat tgagttcctg aaaagcatct gaaagccatt
      601 tttcgggaga gatatttctg tcccttgttc ttccgatgat cgaaaaaaga acagaaccga
      661 agacgatggc accgcctgca ccctgcaggg atttccccat cgcatctgaa ttgatgaaaa
      721 gaatatattt tttcccttcg agaaccagat tctgcgcatg gcacatatct ccgccgattt
      781 catatttttt attatgaaat tcaaatttct ttttctgact gatcagaaac tctgcttccg
      841 ctttttcact ctgcacaaga ttcacaataa aaggctgaat cagaagggat gtgagaaaat
      901 aatctccgtc ctgcataatt ttcagcttct gaatttcggc cagacttttc tgcagatctt
      961 cggttctttc catcactttc agatccaagt ctctgttgag ttccagaaga gattcctgag
     1021 ccttctgctg ctgatacagg aggactgaaa gaagaagcat tgcaatcgaa ttgacaccga
     1081 taaatgtctg cagaatcaga agcttttcat tcagagaata cccgctgaag acaggaaagt
     1141 ttctgagggc tcccagaata agaactgtcg agatgattcc tgaaaaaaga actgcaccgt
     1201 actgcatata acggagagtc gctgccagaa ggaatata
//
LOCUS       DAOQGJ010001573         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91546_length_1238_cov_3.792899, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001573 DAOQGJ010000000
VERSION     DAOQGJ010001573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91546_length_1238_cov_3.792899"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..941)
                     /locus_tag="PK683_19740"
     CDS             complement(18..941)
                     /locus_tag="PK683_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH10740.1"
                     /translation="MIEVKNLTKNYGNKTAIQGLNFTLKKGEIVGLLGLNGSGKTTTI
                     RILTGFLVPSEGEVQIDGKSNFTDPMESKKKIGYLPETPPLYEEMTVREYLDYAGKIK
                     GLSKDALGSEIERVSSMTNLLGVKDTVISHLSLGFRKRVGIAQALLGNPPIIIMDEPI
                     SGLDPKQILDMRNLIQGLAGKHTVLLSSHILSEVQKTCDRFIFLHQGKITHDYKSAEL
                     DIQLAKYALLEVQLSGKKKEECISYLKSIDPGVQVVETLEDSGGLTFGLKVTLPDFTD
                     RLLDTYKQAGLKIGFFKRQTLTLERIFMDNI"
BASE COUNT          336 a          281 c          260 g          361 t
ORIGIN      
        1 atattgttca tagttcctca gatattgtcc atgaaaattc tctcaagtgt gagagtctgt
       61 cttttgaaga aaccgatctt cagaccggcc tgtttgtatg tgtccagaag tctgtctgta
      121 aaatcaggaa gagtcacctt cagtccgaac gtcagcccgc ctgaatcttc gagcgtttcg
      181 accacctgca ctccaggatc aatgctcttc agataactga tgcattcttc ctttttcttt
      241 ccggaaagct gcacttccag aagagcatat tttgccagct gaatatccag ctctgcggac
      301 ttgtagtcgt gagtgatttt tccctgatgc aggaaaataa accggtcgca ggtcttctgc
      361 acttcagaaa gaatatgact ggagagaaga acagtatgct tccccgcaag tccctggatc
      421 agatttctca tatccagaat ctgcttagga tccagtccgg aaatcggttc atccataatg
      481 atgatcggag gatttcccag aagagcctga gcgatcccca ccctctttcg gaaaccgaga
      541 gaaagatgac tgatcactgt atccttcact ccgaggagat ttgtcattga agaaactctt
      601 tcaatttcgg agcccagagc atccttggaa agtcccttga tcttccctgc gtagtccagg
      661 tactcacgga cagtcatttc ttcataaagg ggcggagttt caggcagata accgatcttt
      721 tttttggatt ccatcggatc ggtgaaattg cttttcccgt cgatctgaac ctcgccttcg
      781 gatggcacaa gaaaacctgt aagaattctt attgtagtag tttttcctga accgttcagt
      841 ccaagaagcc ccacaatctc cccttttttc agggtaaaat tcaaaccttg aatggcagtc
      901 ttattgccgt aattcttagt caaatttttt acttcaatca ttgagtcctc attcttaatg
      961 accaaagtac agaaaggcga atgtctgaaa aatgaaaatt tttctgaact taatgaaaag
     1021 tccgcgattc aggcaggtca gaagttttgc aaaatcctag gactgcccgc atcagccgat
     1081 cggttttctc ttgacagaac aaaatgacaa tatcttcttt tgaggtatgg ataactgggc
     1141 ccgaaaattt ctgactgaag aagagtatct gaatcttgaa agaaattcca gttcaaaaaa
     1201 tgagttcttt cgtggagagc tgttcgctat ggcagggg
//
LOCUS       DAOQGJ010001574         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91550_length_1238_cov_3.591716, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001574 DAOQGJ010000000
VERSION     DAOQGJ010001574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91550_length_1238_cov_3.591716"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PK683_19745"
     CDS             complement(<1..1174)
                     /locus_tag="PK683_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10741.1"
                     /translation="MKQKTDWIVSVLGGENIRILLKKTEDAGYKALVLNAAFRFSMEK
                     FMGFLWDSSVTNDKIIKNLSLSVLSDQGSVFGDTAGVVYTKSSDIIIKRLILNLKTYL
                     SAGGTVYENNALITHGVSVYKNQKYPAELKYQLAELYVWLKDLFIPPAELVKNPNLSI
                     AEGLMKNLALIEFTKNTQNLDKSLRDWMNLDLQGVDRFYTVGSDLISSIETTMFLLGI
                     ADTFGYEWNGSVTSVAKVTDMAQATRANGPKGGRLSLLDAAFSMYSNLSGTIGLINIV
                     EGSRTDQTVATAVPGSGTVKVYRDDQPYIINVNTPALSLLEGESRGEVQAASDSIYTK
                     TLPWVLSWISKVVYSGYGPFYNKNRKNSAGNYVTMDGSVYKLANKTDLIYKTSWRTS"
BASE COUNT          342 a          281 c          232 g          383 t
ORIGIN      
        1 gagaggttct ccaggaagtc ttatagataa gatctgtctt gtttgcaagt ttatatactg
       61 atccatccat cgtgacgtag tttcccgcag agttttttct gtttttattg taaaatggac
      121 cataccctga atatacgact tttgaaatcc agctcagcac ccagggaaga gtttttgtat
      181 agatggaatc tgaggcagct tgaacctcac ctctggattc tccttccagc agactgagtg
      241 ccggtgtatt tacattgatt atatacggct gatcatcccg gtatactttc actgttccgc
      301 ttccaggcac tgcagtcgcg actgtctgat cagttctgct cccttcaaca atattgatca
      361 gaccgatggt gcctgaaaga tttgaataca tactgaatgc agcatccaga agactcagtc
      421 ttcccccttt cggtccattg gctctggtgg cctgcgccat atctgtcacc ttggccactg
      481 aggtcactga tccgttccat tcataaccga atgtatcagc tattccaagc agaaacattg
      541 tggtttcaat ggaggatatc agatcactcc ctacagtata aaacctatcc actccctgca
      601 gatccagatt catccaatca cggagagatt tatccagatt ctgagtattt tttgtgaatt
      661 caattaaggc cagatttttc ataaggcctt ctgcaatgga cagattcgga ttttttacca
      721 gttctgcagg aggaataaac agatctttca gccagacata aagttctgca agctgatatt
      781 tcagttcagc aggatatttc tgattcttat aaacagatac gccgtgtgta atcaaagcat
      841 tgttttcata tacagtgccg cctgccgaaa gataggtttt caggtttagt ataagtcttt
      901 ttataattat gtccgatgac ttcgtataaa caactcctgc agtatccccg aacacagaac
      961 cctgatcaga cagaacactc agagaaagat ttttaattat tttatcatta gtaactgatg
     1021 aatcccagag aaaacccata aatttttcca ttgagaatct gaatgcagca ttcaggacta
     1081 gagctttgta tccggcatct tcagtctttt tgagaagaat tctgatattt tctcccccaa
     1141 gaacagagac tatccagtct gtcttctgct tcatcgccaa atctcccctg cttttaaata
     1201 tttcgtcaaa acggaaatgc gtcaagggca tagacata
//
LOCUS       DAOQGJ010001575         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91822_length_1236_cov_3.513971, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001575 DAOQGJ010000000
VERSION     DAOQGJ010001575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91822_length_1236_cov_3.513971"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1236
                     /locus_tag="PK683_19750"
     CDS             <1..>1236
                     /locus_tag="PK683_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10742.1"
                     /translation="ISEETAKAVLNKIQQEISSIPVSEKTFVWFHWWGSSVYSGLTAS
                     DSTAVHMQTSFTEYYPFFAELFLRLSEVETEDMEMKYKTVLPWLNLVARFDFYDRSLS
                     ETIGDKMLELCRQFKYSREFPLHFLSRIKYSNQSFLFDSLLASCRSAGRENVKRFLKS
                     GQHIGKMNQFVYINSFIYIIFTLYSSPSIAVNQLRNLKLTEEDIWLISEAAHHDISNK
                     LTNFSCGEKDFPDWLAEKESAVLFLRELYSGPELSEGQRRLDQLLKRIGSAGTSIWKR
                     QVGISEIPERISEAIEFIGHLKISKDELHFGNTASEKRINTAENKMGQKIPEDLRILY
                     TEMNGILEREISNLDRLVPLQKKFQKKIQCHLKGRKETPAVSENEIDLKTLNEKSPLF
                     ALGEMANGDFLFLPTAVRT"
BASE COUNT          411 a          237 c          262 g          326 t
ORIGIN      
        1 aaataagtga ggaaactgca aaagctgttc tgaataaaat tcagcaggaa atttcttcca
       61 ttcctgtatc cgaaaaaacc tttgtatggt ttcactggtg ggggtcttcc gtatattcag
      121 gactgacggc ttccgacagt accgcagttc atatgcaaac ttcctttacc gaatactatc
      181 cattcttcgc tgaactgttt ttgagactat cagaggtgga gacggaagat atggaaatga
      241 agtataagac agttctcccc tggctgaacc tggttgccag atttgatttt tatgacagga
      301 gtctctctga aacaatcggc gataaaatgc tggagctgtg cagacagttc aagtattcca
      361 gggaatttcc tcttcacttc ctcagcagaa tcaaatacag caaccagtcc tttctgtttg
      421 attcactgct tgcatcctgc agatctgcag gaagggaaaa cgtaaaaaga tttctgaaat
      481 ccggtcagca tataggaaaa atgaatcagt ttgtatatat aaactcattc atatacatta
      541 tttttacatt atacagcagt ccgagtattg cggttaatca attaaggaat ctgaagctta
      601 cagaagagga tatctggctc atatcggaag ccgctcatca tgacatatca aacaaactta
      661 caaatttttc atgcggagaa aaagatttcc cagactggct tgcagaaaaa gaatctgcag
      721 ttctttttct cagagaactt tattcaggcc ccgaactgtc cgaggggcag agaagacttg
      781 atcagcttct gaaacgaatt ggatctgcag gaacatcaat ctggaaaaga caggtcggca
      841 tcagtgaaat tccggagagg atcagtgaag cgattgaatt cataggtcat ctgaagattt
      901 caaaggatga actccatttt ggaaataccg cttcagaaaa gagaatcaat acggctgaaa
      961 acaagatggg acagaaaatt ccggaagatc tgagaatact gtacacagaa atgaacggga
     1021 tactggaaag agaaatttcg aatctggaca ggcttgttcc ccttcagaaa aaattccaaa
     1081 aaaaaattca gtgtcatctg aaaggaagaa aggaaacacc tgcagtttca gaaaatgaaa
     1141 ttgatctgaa gaccttaaat gaaaaatcac ctctttttgc attgggggaa atggccaacg
     1201 gagattttct gttccttcct accgcagtaa gaacag
//
LOCUS       DAOQGJ010001576         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_91866_length_1236_cov_2.448772, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001576 DAOQGJ010000000
VERSION     DAOQGJ010001576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91866_length_1236_cov_2.448772"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1228
                     /locus_tag="PK683_19755"
     CDS             65..1228
                     /locus_tag="PK683_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004487728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10743.1"
                     /translation="MTSEKGEIKIEFTPAVLTKEMIFDSIKVIKTQIENLRVHTFENN
                     YYSFQALNENIFETKGILDNIRFRFFTLGSSNRIEVSRKGNFREEESLAVLNLLKFFS
                     ESETKTVNPKEILSRLGIEVFDPLEAEIKGEAWDFGHMAGYEITKREVQDSIIWPILH
                     PDAFTEITLMTRKYPGKNRPRAVLFEGDPGVGKTTMAKIVSCLCRIPMIYVPIESILS
                     KYYGESSQNLALVFDAAELFPASLLFLDEIDSLAGRREDGMFEATRKLLSVLLRKLDG
                     FEGKPNTVTIGATNRKQDLDSALVSRFDKSILFPLPDGRERTAILGNYAVHLSEDERN
                     RISSKMEGLSGRKIRDFCDFTERRWVSLLLESGKKIEPPPAEIYLEAMEIYRR"
BASE COUNT          403 a          248 c          277 g          308 t
ORIGIN      
        1 gaaagaactc atatttcggg aaacgaaaga cagtaaaggg gaaaactctg tcagaggact
       61 ctctgtgact tcagaaaaag gtgaaatcaa gattgaattt acccctgcag tccttaccaa
      121 ggaaatgatt tttgacagca tcaaagtcat caagactcag attgaaaatc tgagagtcca
      181 tactttcgaa aacaattatt attcatttca ggccctgaac gaaaatattt ttgaaaccaa
      241 gggcattctg gacaatatca gattccgctt ttttacactg ggttcatcca acaggataga
      301 agtttcaaga aaaggcaatt tcagagaaga agaatctctc gcagtgctga accttctgaa
      361 atttttctca gaaagtgaaa caaaaacagt caacccgaag gaaattctct ccagactcgg
      421 tattgaagtt tttgatcccc ttgaggcaga aatcaaggga gaggcctggg atttcggaca
      481 tatggccggt tacgaaatca caaaaaggga agttcaggat tccattatct ggcctatact
      541 gcacccggat gcattcactg aaattactct gatgaccaga aaatatcccg gaaaaaacag
      601 accgcgtgcc gttcttttcg agggtgatcc gggagtagga aagactacga tggcaaaaat
      661 cgtctcctgt ctctgcagaa tcccgatgat ttatgttccg atagaatcga ttctcagcaa
      721 atactatgga gaaagttctc aaaatcttgc actggttttt gatgccgcag aactttttcc
      781 ggcaagcctt ctttttctgg atgaaattga ttcgcttgca ggacgcagag aagacggtat
      841 gttcgaggca accagaaaac ttctttcagt tctgctcaga aaactcgacg ggtttgaagg
      901 gaaaccgaat acagtgacaa tcggtgcaac aaaccgaaag caggacctgg attcggccct
      961 ggtttccaga tttgacaaga gcattctgtt tcctctaccg gacggacggg aaagaacagc
     1021 aatactgggt aattatgctg tacacctaag cgaagatgaa cggaaccgga tttcatcaaa
     1081 gatggaagga ctttcaggaa gaaaaatcag ggatttctgc gattttacag aacgaagatg
     1141 ggtttctctt cttctggaaa gcgggaaaaa aattgaaccg cctcctgcag agatttatct
     1201 ggaagcaatg gaaatctaca ggagataaaa cataat
//
LOCUS       DAOQGJ010001577         1234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92095_length_1234_cov_2.938931, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001577 DAOQGJ010000000
VERSION     DAOQGJ010001577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92095_length_1234_cov_2.938931"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..821
                     /locus_tag="PK683_19760"
     CDS             132..821
                     /locus_tag="PK683_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10744.1"
                     /translation="MKDYLLKKFWGGVLFSIGALSTAVMAVAVTGTITSFNSGDKVSS
                     SKVNENFLTLKTAIESIPEWTKSGTDAVFSGGNISVGTTTAVSGTRVTVNGRISSSVL
                     GVYCGNTSGIGTSGSFTNGSTAGAISYNGKTGYRGAKAACETACSNTNAHMCTSHEIA
                     ISRQLGLTIDPTEQMISIGGFMYDGTYYATDCDGWTSASSTKIGIVTNHTNGTYWSIA
                     CNGLKLLTCCL"
     gene            complement(841..1173)
                     /locus_tag="PK683_19765"
     CDS             complement(841..1173)
                     /locus_tag="PK683_19765"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNH10745.1"
                     /translation="MKLLFDCNLSHKLPARLADLYPGSTHPRIYGNETDGDEVIWLYA
                     KENDFIIVTRDDDFHHLSSVYGSPPKLIWIRHSNSPTGDIEKMLRINYENISVFYNNP
                     KLHCLFLW"
BASE COUNT          397 a          226 c          273 g          338 t
ORIGIN      
        1 atcgaggtgg gcggtgaaaa tcactcggta acctgctttt acacagtccc acttcgggag
       61 gatcacactc agcttgcgaa gcaagctgca gataggacag caatatatag ttatataaag
      121 ggagacacac tatgaaagat tatcttttaa aaaaattctg gggcggagtt cttttttcga
      181 taggagcttt atcaactgct gtcatggctg tggcagtgac aggaacaatc acatccttta
      241 actcgggaga taaagtatct tccagcaaag taaatgaaaa ttttttaacg ctgaagactg
      301 caatagaaag tattccagaa tggacaaaaa gtggaactga tgcagttttt tctggaggaa
      361 atatttctgt cggtacaacg acggctgttt ccggaacacg ggtcacagtg aacggcagaa
      421 tctcttctag tgtgcttggg gtttattgcg gcaatacttc aggaatcggt accagtggat
      481 cttttacaaa tggaagtact gctggagcaa tcagttataa cggaaaaaca ggatacagag
      541 gtgcaaaagc ggcctgtgaa actgcttgca gcaatacaaa tgctcatatg tgcacaagtc
      601 atgaaatagc aatcagtcga cagctaggct taaccattga tcctacagaa caaatgattt
      661 cgataggagg atttatgtat gatggaactt actatgctac agactgtgat ggctggacaa
      721 gtgcttccag tactaagatt ggtatcgtta caaaccatac taatggtact tattggagca
      781 tagcctgtaa tggactgaaa cttttaacct gctgtcttta aaaaaatatc tgatagttta
      841 tcaccaaaga aacagacaat ggagtttggg attattataa aagacagata tattttcgta
      901 attgattcga agcatttttt caatgtcccc tgtaggggaa ttgctgtgac gaatccagat
      961 cagtttcgga ggagatccat atacagaact caaatgatga aaatcatcat cacgggtaac
     1021 aatgatgaaa tcgttttctt tggcatataa ccaaatgact tcgtcgccgt cagtttcatt
     1081 tccgtatatt cgaggatgtg tagaacccgg atacaaatca gccagccttg ctggaagttt
     1141 atgggacaaa ttgcagtcga ataaaagctt cagtttagac caccatcgta aactgctctc
     1201 tgtctacaat ataagcgaaa caggcaagaa tatc
//
LOCUS       DAOQGJ010001578         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92371_length_1232_cov_2.918437, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001578 DAOQGJ010000000
VERSION     DAOQGJ010001578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92371_length_1232_cov_2.918437"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PK683_19770"
     CDS             complement(<1..808)
                     /locus_tag="PK683_19770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH10746.1"
                     /translation="MSDKRKIVVIGGVAGGATAAGRARRMNESAEITVLERGGEISFA
                     NCGLPYHISGDIPERKSLILRSAKAFWDRYRILAKTNVEVTRIDRTAKVVFFRESGKE
                     DKISYDKLILSQGAAPMLPPVTGLENIPAFSLRTLEDMDSINLQTLNSSSKNVIVVGG
                     GFIGVEMAEAFVKRGFTVDLVEFAPQIMISVDSEFAQMAEEEMIKHGVKVHKGTKIES
                     VSKEKEVLLANGIKIHADFILFAAGVRPELELAKNAGLKIGEFGGLSVNEY"
     gene            complement(818..1072)
                     /locus_tag="PK683_19775"
     CDS             complement(818..1072)
                     /locus_tag="PK683_19775"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNH10747.1"
                     /translation="MEKKELIHRLNRIQGQIEAVRRMLESEETDCKKTIELVKASSNA
                     LKKFAQAYMESHIEDCLAENRKVKDIRKDLKEVVAASFTL"
BASE COUNT          317 a          317 c          229 g          369 t
ORIGIN      
        1 gatattcatt tacagaaagt cctccgaatt caccgatctt cagtcctgca ttctttgcaa
       61 gttcaagctc aggtctgact ccggctgcaa ataaaatgaa atcagcatga atttttatcc
      121 cattggcaag aaggacttcc ttctccttgg aaacagactc gatctttgtg cccttgtgca
      181 ccttgactcc gtgttttatc atttcctctt cagccatctg tgcaaattcg gaatcgacag
      241 agatcataat ctgaggggca aattcgacca gatccacagt aaatcctctt ttcacaaatg
      301 cttccgccat ctcgactccg atgaatccgc ctccgaccac tatgacattt tttgaagaag
      361 aattcaatgt ctgcagattg atcgaatcca tatcttccag agttctgagt gaaaatgctg
      421 gaatattttc aagtcctgtc acaggaggaa gcataggagc agctccctga gagaggatga
      481 gtttatcgta agagatttta tcttcttttc ctgattccct gaaaaaaaca actttggctg
      541 ttctgtctat tcgggtgact tccacattcg tcttcgcaag gattctgtat ctgtcccaga
      601 aagcctttgc ggatctcaga atcagacttt ttctctccgg aatgtccccc gaaatatggt
      661 aaggaagtcc gcagttggca aaagaaattt caccgcctct ttccagaact gtaatttcag
      721 cagactcatt catccgtctg gcacgtcctg cagcagtggc tccccctgcc acacccccga
      781 tgactacaat ttttctttta tcagacatac aattctccta taaagtgaag gatgctgcca
      841 caacttcttt cagatccttt cgaatatcct tgacttttct gttctctgcc agacagtctt
      901 ctatatgaga ttccatatac gcctgtgcaa atttcttcag agcattcgag gaagccttga
      961 cgagttcaat cgtcttcttg cagtcagtct cctcagattc cagcatcctg cgcactgcct
     1021 caatctgccc ctgtatcctg ttcagccggt gaatcagttc tttcttttcc ataagccaat
     1081 aatattcaga tcataaattc tgtcaagcaa ttaccatacg gggtatggta tatgttccga
     1141 aaaggatcat cgatgctgtg caggtcatcg actcaggaaa ggttcagtaa actcaccgca
     1201 ccgctcggcc aatgctgaca attaatgcga ct
//
LOCUS       DAOQGJ010001579         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92572_length_1230_cov_4.919149, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001579 DAOQGJ010000000
VERSION     DAOQGJ010001579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92572_length_1230_cov_4.919149"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PK683_19780"
     CDS             <1..340
                     /locus_tag="PK683_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10748.1"
                     /translation="GESKMNFFSLVLLGISAFSVYFDLLSIRILIFSAMNMSLVLLFV
                     IILVYIKLFTNYAVPGWTSVVATNMLLLFGQMVIIAAFTLISFLNRRSSYKIIPSKVY
                     RDYIKEIEEI"
     gene            340..1032
                     /locus_tag="PK683_19785"
     CDS             340..1032
                     /locus_tag="PK683_19785"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH10749.1"
                     /translation="MSNIDYIGSELEIFQYAVNWKEYWSSSIVKFIKGDVLEVGAGIG
                     TNTIIFKNTHFCSWTSVEPDRKLVEKLKQNLNDSSYNVVHGTIKDTPGDSFYDTILYI
                     DVIEHIEKDREELLNAYEKLKVGGHLIILVPAHNFLYSEFDKSIGHFRRYSRKELLYV
                     IPAELNLLNIRYLDSLGVMASTVNKVFLKQPSPNKSQILFWDRVIIPISRIMDRILFY
                     KMGKSLVGIWKK"
     gene            1020..>1230
                     /locus_tag="PK683_19790"
     CDS             1020..>1230
                     /locus_tag="PK683_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10750.1"
                     /translation="MEKVNMKLFKILFAVMTVLLLSYTTFYKYSADLFVDNDNIKIYR
                     IVQDGKLPWFTGVNPPDYFSQKQRPF"
BASE COUNT          377 a          181 c          239 g          433 t
ORIGIN      
        1 tggagaatca aaaatgaatt ttttttcctt ggttcttctg gggatttccg cattttctgt
       61 gtactttgat cttctgagca ttcgtatact aattttttct gcaatgaata tgtctttagt
      121 tcttttattt gtaattatac tggtctatat taaattgttt acgaattatg ccgtccctgg
      181 ctggacatca gttgttgcaa caaatatgct tttgctgttt ggtcagatgg ttattatcgc
      241 agcctttact ttaatttctt ttttgaacag gagaagcagt tacaagatta ttccttcgaa
      301 ggtgtacaga gactatatca aggaaattga ggaaatataa tgtccaatat tgattacata
      361 ggatcagaac ttgaaatatt tcagtatgct gtgaattgga aggaatattg gagttcttca
      421 atagtaaagt ttattaaagg ggatgttctt gaagttggtg cagggatagg aacaaacacg
      481 ataattttca aaaatactca cttttgctcc tggacaagcg ttgaacctga ccgaaaatta
      541 gttgagaaac tgaaacagaa tcttaatgac agcagctata atgttgttca tggaacaata
      601 aaggatactc cgggtgattc cttttatgat acaattttat atatagacgt aattgaacat
      661 atagaaaagg acagagaaga gcttctgaat gcttatgaaa aattgaaagt cggaggacat
      721 ttgattatac tcgtcccggc ccataatttt ttatattccg aatttgacaa atctatagga
      781 cattttcgaa gatacagcag gaaagaactg ctgtacgtca ttccggctga attaaattta
      841 ttgaacatcc ggtatttgga ttctcttggt gtgatggctt ccactgtaaa taaagttttc
      901 ctgaagcagc cttctccgaa taagtctcaa atattgtttt gggatagagt tattattcca
      961 atttcaagaa taatggacag aattttattt tataagatgg ggaaatcatt ggttggaata
     1021 tggaaaaagt gaatatgaag ctttttaaaa ttctttttgc agtgatgaca gttttactgt
     1081 tgtcctatac taccttttat aagtattcag ctgatctttt tgtcgataac gataatatta
     1141 aaatatacag aattgtgcag gatggtaaac ttccctggtt tacaggggtt aatcctccgg
     1201 attatttttc ccagaaacag cgtcccttcg
//
LOCUS       DAOQGJ010001580         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92589_length_1230_cov_3.496170, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001580 DAOQGJ010000000
VERSION     DAOQGJ010001580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92589_length_1230_cov_3.496170"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PK683_19795"
     CDS             <1..579
                     /locus_tag="PK683_19795"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HNH10751.1"
                     /translation="PVPGTYMEQWIAGPRMKSAFKPYSPLTENINSLAENLGKSNDEL
                     RIVVQDRPRHDKLIKELRELGCSVCLIDSGSVTAVLDICLEKGMYDALIGTFGAPEGL
                     ISAVIAKITHSEMKGILRPHDEAFKKRAEDHGFKEEKVLDKNEWVPGEKIGFLASCLS
                     PNHFMKGLKVDHHIISGETLVLYENHSSVEGF"
     gene            579..>1230
                     /locus_tag="PK683_19800"
     CDS             579..>1230
                     /locus_tag="PK683_19800"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNH10752.1"
                     /translation="MEPPFQNDYIFGNSLLEAIPHPDGFFGSILYGLSVAAHFSGGPN
                     FFTLFMPFILYFYSMRFGWKLMIAICSTGVFNGLAKFYFQSPRPHHLSQTFDQFQSQV
                     HEGSFGLPSGHTQVSIGVWLLVFFEFKNVYIRSFAVFMALFTPFSRMYAGVHYPGDII
                     GGALLAGLTLLIIHYMFLDNQEFPDINSWKNSAVPVRSRIFGLTAVCIAPILLQNQN"
BASE COUNT          306 a          258 c          278 g          388 t
ORIGIN      
        1 ccagttccag gaacatatat ggagcagtgg attgccggac ctagaatgaa atctgccttc
       61 aagccatatt ccccactgac ggagaacatt aattccctgg ctgaaaatct gggaaagtcc
      121 aacgatgaat tgagaattgt tgttcaggac agaccaagac atgataagct gattaaggaa
      181 cttagagagc ttgggtgctc agtctgtctg attgattccg gatccgtgac ggctgttctg
      241 gacatttgtc tggagaaagg tatgtatgat gccctgattg gtacttttgg tgctccggaa
      301 ggtctgattt cagcggtgat tgcaaaaatt actcactccg aaatgaaggg aattcttcgc
      361 cctcacgatg aagcttttaa gaaaagagca gaggaccatg gtttcaaaga agaaaaagtt
      421 ctggataaaa atgaatgggt gcccggcgaa aaaatcggtt ttcttgcctc atgtctcagt
      481 ccaaatcatt ttatgaaagg acttaaagtg gatcatcata ttatttccgg tgaaacattg
      541 gtattgtatg aaaatcacag cagtgtagag ggattctgat ggagcctcct tttcaaaacg
      601 attatatttt cggcaacagt ctgctcgaag caattccgca tccggacggg ttttttggaa
      661 gcattcttta tgggctttcc gtcgcagcac atttttccgg cggccccaat ttctttaccc
      721 tctttatgcc tttcattctc tatttttaca gtatgaggtt cggctggaag ctgatgattg
      781 ccatctgctc taccggtgtc tttaacggat tggctaaatt ctattttcag agtccgcgtc
      841 cgcatcatct ctcccagact tttgatcagt ttcagtcaca ggttcatgaa ggttcattcg
      901 gtctgccttc aggccatacg caggtctcaa tcggtgtctg gcttcttgtg ttttttgaat
      961 ttaagaacgt atatatcaga tcgttcgcag tttttatggc tttgtttact cctttcagcc
     1021 ggatgtatgc cggagtgcat tatcctggag acattatcgg gggagcgctg cttgccggtc
     1081 ttactctttt gatcatacat tatatgtttc ttgataatca agaattccct gatataaact
     1141 cctggaaaaa ttcagctgtt cctgtcagat ccagaatctt cggtctgact gctgtctgta
     1201 ttgcaccgat tctgcttcag aaccagaatt
//
LOCUS       DAOQGJ010001581         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92590_length_1230_cov_3.463830, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001581 DAOQGJ010000000
VERSION     DAOQGJ010001581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92590_length_1230_cov_3.463830"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1183
                     /gene="fliG"
                     /locus_tag="PK683_19805"
     CDS             173..1183
                     /gene="fliG"
                     /locus_tag="PK683_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNH10753.1"
                     /translation="MDNYKPSSKGAKKAAMLLMSLGKEEAAKVMAHLDDKMIEEVIQE
                     MSRIRTVSKFEKENILSEFRETVEEVKNGSKGGVDAAKELLSKSLGSHKAEEFFRKME
                     KKDVSQEFEFLNEVEPNVIQSLLSSEAPQTIAVTLSYLVPKKAAEVLKLFPQEQQSAI
                     ALKLATTSKSFPEAILEIAKVLRKKYDARDKSEMTEAGGVQSLANILNHMDKDIEENI
                     LKNLNQESPEVAHQVRDKLYAFEDILNLEGKEMRMLLTRLSGNELLTLALRGAGDEMK
                     RHFFNSMSQNRASDILEEMEARGKVTLREINAARSEILKIARKLEEQNQLILKKRKEE
                     FI"
BASE COUNT          418 a          251 c          284 g          277 t
ORIGIN      
        1 gtttaatacc tcgacaggct cggcacatcg ctcggtgacc gcggtgaagc cttcttcgaa
       61 atatgctttt acacagtccc taatttacaa tatcatccag ccaaagtata tcgccttccg
      121 gctcaaaaat tctaattgaa aaaaacttgg tactgaatca gtctaggcct caatggacaa
      181 ttataaacct tcttccaaag gggccaaaaa ggccgcaatg cttctcatgt ctttaggcaa
      241 ggaagaagca gcaaaagtga tggcacatct cgacgacaag atgattgaag aggtcattca
      301 ggagatgtcc agaatcagga cagtatccaa attcgaaaaa gaaaacattc tttctgaatt
      361 cagggagact gttgaagaag taaaaaacgg ctcaaagggc ggggtcgatg ctgcaaagga
      421 acttctttcg aaatcactcg gttctcacaa ggcagaagag tttttccgga aaatggaaaa
      481 aaaggatgtc tcccaggaat ttgaatttct gaatgaggtg gagccgaatg taatccagtc
      541 acttctctcg agtgaagcac ctcagacaat tgcagtcacc ctatcctacc ttgtcccgaa
      601 aaaagcggca gaagtactga aactatttcc gcaggaacag cagtcagcga tcgcattgaa
      661 gctggcaaca acatccaagt cctttcctga agccatatta gaaattgcta aagttctcag
      721 aaagaaatat gatgcgagag acaagtctga aatgactgaa gcaggaggag tccagtccct
      781 tgcaaacatt ctgaatcata tggacaagga tattgaagag aacatactga aaaatctgaa
      841 ccaggaatct cctgaagtgg ctcatcaggt cagggacaag ctgtatgcct ttgaagatat
      901 actgaatctg gaaggtaaag aaatgagaat gcttcttact agactttccg ggaatgaact
      961 tctcactctc gcccttcgcg gtgcaggaga tgagatgaaa aggcattttt tcaattcaat
     1021 gtcccagaac agagcatcgg atattctgga agaaatggaa gcaaggggaa aagtgacttt
     1081 aagagaaatc aatgcagcaa gatccgaaat actgaagatt gcaagaaaac tggaagaaca
     1141 gaatcagctg atattgaaga agagaaaaga agaatttatc tgaggacgga gctgacggga
     1201 ctcgaacccg cgacatcctg cgtgacaggc
//
LOCUS       DAOQGJ010001582         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92597_length_1230_cov_3.320000, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001582 DAOQGJ010000000
VERSION     DAOQGJ010001582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92597_length_1230_cov_3.320000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1230
                     /locus_tag="PK683_19810"
     CDS             <1..>1230
                     /locus_tag="PK683_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10754.1"
                     /translation="TVSTILTALITFGAAVFLLKLPNHSRATTYLGLMFLFIAIPGTG
                     YFLNNGLYYPSSFSRYLTLISIPMRFTLLAQFLIYFPRLRNPKFAKWSLIVQCIISAL
                     LGIWLLAVGPSSELYFDLSGQSYDLKVPLFFKGYSVFLILFILFAMGIAVRKALTHKG
                     EERTAVIIIIIALILDMVIPTAINAMNRNGYISRDALMAALGMLTPVGTFLILIAFIN
                     TTKDKSTFLFKIVGISFLTFVFVYNIIIYVFMQDKEFSYDEDRVNDLKLVLKAGERPA
                     NLDYLMEYRPSTGEIKFSYNRNETQIAEFEPKLINSYFVESLRLFPENFTAQEIIAKL
                     DSIPIRNSQYLEPHKLSLKDYLSSYSGKKPVNDSVQFLVSKERTLYYHSNKIKEMSEK
                     DFRDELKAYFAKTPPEMA"
BASE COUNT          354 a          276 c          234 g          366 t
ORIGIN      
        1 acagtcagta ctattttaac cgcactgatt accttcgggg ctgccgtttt tctgttaaaa
       61 ctcccaaatc attcgagagc aacaacttac ctgggactga tgtttctttt cattgcaatc
      121 cccggaaccg gttattttct gaataacggt ctttattatc cgtcctcatt ttccaggtat
      181 ctgactctga tttccattcc aatgcgtttt actctccttg cccagtttct aatctatttt
      241 cccagactca gaaaccccaa atttgccaaa tggtctctga ttgtacagtg tattatttct
      301 gcactgctcg gaatctggct ccttgctgta ggtcccagct cagagctgta ttttgatctg
      361 tctggacaaa gttacgacct taaggtcccc ctgtttttca agggttacag cgtttttctg
      421 atcctgttta ttctgttcgc aatggggatt gccgtcagaa aagcactgac tcacaaagga
      481 gaagaaagaa cagcagttat tattatcatt atcgcactga ttctggatat ggtgattccg
      541 acagccatca atgcaatgaa cagaaacggc tatatcagca gagatgcact gatggctgcc
      601 ctgggaatgc tcaccccagt cggcacattt ctgattctga tcgcattcat caatacaaca
      661 aaggataaaa gcacatttct tttcaagatt gtcggaatca gttttctgac ttttgttttt
      721 gtttacaata tcattattta tgtctttatg caggacaagg aattttccta tgatgaggac
      781 agggtaaacg atctgaaact tgttctgaag gcaggcgaac gtcctgcaaa tctggactac
      841 ctgatggaat acagaccgtc caccggagaa ataaaattca gctacaacag gaatgaaact
      901 cagattgctg aatttgaacc caaactgatc aattcctatt tcgtggaatc attgagactg
      961 tttccggaaa acttcacagc acaggaaatc attgcaaaac tggattccat cccgatccga
     1021 aattcccagt accttgagcc gcataaactc tcactgaagg attatctgag ttcctattca
     1081 ggaaaaaaac cggtcaacga ctcggttcag tttcttgtct cgaaggaaag aaccttatac
     1141 tatcattcca ataaaataaa ggaaatgagt gaaaaagact tcagagatga actgaaggct
     1201 tattttgcaa aaacacctcc ggaaatggca
//
LOCUS       DAOQGJ010001583         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92653_length_1230_cov_2.304681, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001583 DAOQGJ010000000
VERSION     DAOQGJ010001583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92653_length_1230_cov_2.304681"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PK683_19815"
     CDS             complement(<1..635)
                     /locus_tag="PK683_19815"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNH10755.1"
                     /translation="MEGKLKGNNLGKEVIMFRKFAVLGLLSVSVSVFSADCDLSFDPS
                     STVLKWKAFKFTEKTGVEGSFTKVEALESKSAKSVVLLAEGLKFSIDTASVHTNNPDR
                     DAKISKFFFGTMKETSKITGNFRKVKLSGSSGTADLVLKMNGETKTVPVKFTVADNVK
                     IELKGTVDLNLFRAQKSVDSLNKECNAQHIGKDGKSKLWNDVEISLNTVLK"
     gene            886..>1230
                     /locus_tag="PK683_19820"
     CDS             886..>1230
                     /locus_tag="PK683_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10756.1"
                     /translation="MAIALFAGAHLSSKKDALWIPVAGMFLSDLFLGFHSLQILVYGL
                     MVCTVILGWQLRSISSPARILGTSLFGSVLFFAVTNLFVWLTSGMYELNLNGFIQCYT
                     LAIPFFQNSLAGD"
BASE COUNT          311 a          272 c          229 g          418 t
ORIGIN      
        1 tgcttgagca cagtattcag actgatttct acatcattcc agagcttcga cttaccgtct
       61 tttccgatat gctgagcatt acattccttg ttcagagagt ccacagactt ctgtgcacga
      121 aaaagattca agtccactgt tcccttcagt tcgattttta cattatcagc cacagtgaat
      181 ttcaccggaa ccgttttagt ttctccgttc atctttaaaa ccagatcagc cgttcctgaa
      241 ctgcctgaga gcttcacttt ccgaaagttc ccggtaatct tagaagtttc cttcattgtc
      301 ccgaagaaga attttgaaat ttttgcgtcc ctgtccggat tgtttgtatg cacagatgca
      361 gtatcaatac tgaatttcag tccctctgcc agaagaacaa ctgattttgc agatttactt
      421 tccaaagctt ccactttagt gaaacttcct tccacacctg ttttttcagt gaatttaaag
      481 gccttccatt tcaaaacggt ggatgaaggg tcaaaagaaa gatcacagtc tgcagaaaaa
      541 acagataccg atactgacaa tagaccaaga actgcaaatt ttctgaacat aattacctct
      601 tttccgagat tatttccctt cagttttcct tccacaaata attactgatt tttttacttg
      661 aattaaaatg cccgaccgct gttattacaa atagtatccg tttgcgaaaa ttgatactgc
      721 agcatgatcc ggaaattctc acaaaagccg gttccatgtt acttatatct tctgatttta
      781 gaggtgagat tcatgtggaa ttcacgtttt tttattgtta cttctctgat tctggcagct
      841 gcagtgagcc gactcattca tcttccggga aatttttctc cagttatggc aattgcactg
      901 tttgcaggag cgcatctttc cagtaaaaaa gatgcccttt ggattccagt ggcaggaatg
      961 ttcctgagtg atctgtttct gggatttcat tctctgcaga ttcttgttta cggactgatg
     1021 gtatgcacag tgattctcgg ctggcagctt cgttcgattt cttcgcctgc aagaatcctg
     1081 ggaacttctc tttttggttc tgtcctgttt tttgctgtga caaacctgtt cgtatggctg
     1141 acatcaggaa tgtatgaact gaatctgaac ggattcattc agtgttatac acttgccatt
     1201 ccattttttc agaacagtct ggcaggtgat
//
LOCUS       DAOQGJ010001584         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92736_length_1229_cov_3.460818, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001584 DAOQGJ010000000
VERSION     DAOQGJ010001584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92736_length_1229_cov_3.460818"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1229
                     /locus_tag="PK683_19825"
     CDS             <1..>1229
                     /locus_tag="PK683_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10757.1"
                     /translation="FFITVFPLFSEKIVPQKISYLNESARAEISEISFGKTAPRRFEE
                     IKNIREPELKDFQLKMPSDIPSVHIGPDGGEVRIISKNNYQWKRMDQSLFAERPDGSW
                     QLTLTDGTVFSSLEPCAGCMPSTKILYKDGTELYKKWIKHRKEHDWIFQKEDEAPALH
                     WKLTDSSKFQHSAKFSKYTFYYSSEWDSFIKALKERFPGDAFFDYMKNEFGLENAGQV
                     PVILFSDRKSLTAYAGRDLPGGEKKNGNGGGGFGGRDSIVIAIDKSVRPLTGDSEVDD
                     YIIKKISFRTLYHESIHNLIQLSCVSQRIGKTDLKPYIHDPWFNEGTANFSIQKFYPA
                     MTAEVYNGLDDQIKNNKIPSSYSVLMKKKYDDLLPYRLGAYMVEYLYRTYGSESFKEI
                     NKGICLGNSAEEAIRKT"
BASE COUNT          387 a          222 c          283 g          337 t
ORIGIN      
        1 tatttttcat aaccgtattt cctctattct ctgaaaaaat tgttcctcag aaaatcagct
       61 atcttaacga gtctgcaagg gcagagatca gtgaaatcag tttcggaaaa actgccccaa
      121 gacgttttga ggaaattaaa aatatcagag aacctgaact taaggatttt cagctgaaga
      181 tgccttcaga tattccttcg gtccatatcg gtcctgacgg cggggaagtc agaattatat
      241 ctaaaaataa ttatcaatgg aagagaatgg atcagtctct gtttgcggag agaccggatg
      301 gaagctggca gctgactctt acggacggaa ctgttttttc atcgcttgag ccttgtgcag
      361 gatgtatgcc gagcacaaag attttataca aggacgggac agaactctac aaaaaatgga
      421 taaagcacag aaaggagcat gactggattt tccagaaaga agacgaggct cctgctctgc
      481 attggaagct gactgattct tctaaatttc agcacagtgc caaattctcc aagtatacct
      541 tctattattc ttctgaatgg gattcattta ttaaggcatt gaaagaacgt tttccgggtg
      601 atgctttttt tgattatatg aaaaatgaat ttggacttga aaatgcaggt caggtaccgg
      661 tcatcttatt cagtgacaga aaaagtctga cagcttatgc aggacgggat cttcccggag
      721 gcgagaagaa aaacgggaac ggcggaggcg gttttggcgg aagagattct atagtaatag
      781 cgattgacaa gtcagttcgg ccgctgacag gagattcgga ggtcgatgat tatatcatta
      841 aaaagatcag cttcaggact ctgtatcatg aatccattca taatcttatt cagctttcct
      901 gtgtttctca aaggatcggc aagacagact tgaagccgta tatacatgat ccctggttca
      961 atgaaggcac tgccaatttt tccattcaga aattttatcc ggcaatgact gcagaagttt
     1021 ataatggact ggatgatcag attaaaaata ataaaatccc ttccagttac tccgttctga
     1081 tgaagaagaa atatgatgat ctgcttccat acaggctggg cgcctatatg gtggaatatc
     1141 tgtacagaac ttacggaagt gaaagtttca aggaaataaa caaaggaatc tgtcttggga
     1201 acagtgctga ggaggcaata aggaagaca
//
LOCUS       DAOQGJ010001585         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92738_length_1229_cov_3.406303, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001585 DAOQGJ010000000
VERSION     DAOQGJ010001585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92738_length_1229_cov_3.406303"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..>1229)
                     /locus_tag="PK683_19830"
     CDS             complement(55..>1229)
                     /locus_tag="PK683_19830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH10758.1"
                     /translation="LFVPMFIAEFILRLWQISALFFAVPVMIIANAKAIRKRIPSGKT
                     ILLAFTVTITTAVFDILDSLIMNTGYGFTKYSFFLFIMTLMLILARKFVELHNRTEEL
                     NLNLEHKVEERTKELKNTLQTVNDLKVQQDGDYFLTSLITEPLGLNMNKSIIIKTDMY
                     ISQKKKFEFRKRRGELGGDICVTGNLRFGTMENRYVMFFNGDAMGKSMQGAGGAIVAG
                     TVVNNIMSKSARNNRVLTEVTPEKWLKATFNELDNAFRTFNGSMLVSGVMGLISEQTG
                     ELLYFNAEHPYLVLYRDGIADFIDKEIYLRKLGTELIEKFTIIRFQLLPGDVLFAGSD
                     GKDDIKMGEHKDMNEDERLFLRQVEKSRGELEILSRMIHNSGEIADDLSLLKISYQ"
BASE COUNT          324 a          268 c          235 g          402 t
ORIGIN      
        1 taaatcccta tttagcttcg gatgaaagat aatttcttga ttttttcaac aagtctattg
       61 ataactgatt ttgagaagag aaaggtcatc tgctatttct cctgaattat ggatcattct
      121 ggaaagaatt tccagctcac cgcgcgactt ttccacctgt ctgagaaaaa gtctttcatc
      181 ctcattcata tccttatgtt ctcccatctt gatgtcgtcc tttccgtctg atccggcaaa
      241 cagaacatct cccggcagaa gctgaaaacg gatgattgta aatttttcaa tcagctcagt
      301 tcccagtttt ctcagatata tttccttatc aatgaaatca gcgattccgt ctctgtagag
      361 gacgagatac ggatgttctg cattgaaata cagaagttcg cctgtctgtt ctgaaatgag
      421 ccccattacc cctgaaacca gcatcgatcc gttgaacgtt ctgaatgcat tgtccagttc
      481 attgaatgtg gctttcagcc atttttcagg agtcacttca gtaagcaccc tgttgtttct
      541 ggcagacttg ctcataatat tgtttacgac tgtaccggca acaatggctc cccccgcccc
      601 ctgcattgat tttcccattg catcgccgtt gaagaacatt acatacctgt tttccattgt
      661 tccgaatctg agatttcctg tgacgcagat atcgcctccc agctctcccc ttcttttcct
      721 gaattcaaac ttttttttct gggatatata catatcagtt ttaatgataa tagatttatt
      781 catattcaga cccagaggtt cagtgataag agatgtaagg aagtagtctc cgtcctgctg
      841 cactttcaga tcgtttacag tctgaagtgt gtttttcagt tctttagttc tttcctccac
      901 cttgtgttca aggttcagat tcagttcctc agttcggttg tgaagttcca caaactttct
      961 ggcgaggatc agcataagag tcattatgaa cagaaagaaa gagtatttag taaatccata
     1021 tcctgtattc ataatcaggg agtccagtat gtcgaagaca gctgttgtta ttgttacagt
     1081 gaatgcaaga aggattgttt ttcctgaagg aattcttttt ctgattgctt ttgcgtttgc
     1141 aataatcatt acagggactg caaagaaaag tgcactgatc tgccagaggc gcagaatgaa
     1201 ctcagcaatg aacatcggaa caaagagag
//
LOCUS       DAOQGJ010001586         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92808_length_1229_cov_2.378194, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001586 DAOQGJ010000000
VERSION     DAOQGJ010001586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92808_length_1229_cov_2.378194"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..441)
                     /locus_tag="PK683_19835"
     CDS             complement(169..441)
                     /locus_tag="PK683_19835"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNH10759.1"
                     /translation="MVWKIAAAKSQFSELVSQAEKEPQVVYNRKKPSVVVLSFEEYTR
                     LHSLDVLIHRPPKWSEFTAFSEKLADEKCSAAIELPSRKDRNSSVF"
     gene            623..1012
                     /locus_tag="PK683_19840"
     CDS             623..1012
                     /locus_tag="PK683_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNH10760.1"
                     /translation="MKSKFLAISVILAASVLSIAYFSTKGEASFLLLDASELAVNPAK
                     YSGENLRVRGNVKIGSVLREGREARFVLELNGKEVPVHFTGKNLLPDAFKDGVRARVD
                     GHFKSGTLEADHVEAKCASKYEADYSK"
     gene            1009..>1229
                     /locus_tag="PK683_19845"
     CDS             1009..>1229
                     /locus_tag="PK683_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10761.1"
                     /translation="MTLNDLGSAVMVVSCGLTAFSLFQTVYGMLRHDESAVRLGQTSL
                     LANFGVILLAFVILLTALARMDLTNHYAV"
BASE COUNT          319 a          265 c          275 g          370 t
ORIGIN      
        1 gtcttttctt ctgctcctgt tttgcccgat tgattccata acgcagttca tggaaggaaa
       61 tgacgctgaa tagaagattt tcctgagcgg atatccattt tactgcacca gtatttcttc
      121 ccggtttagt aagttctgat ataatatttg tatcaatgag atacatgact aaaaaacgga
      181 actgttcctg tctttgcggg acggaagttc aattgccgcc gaacattttt catcggcaag
      241 tttttcggaa aaggcagtaa attcagacca tttcggaggt ctgtgaatca gtacatccag
      301 gctgtgcaga cgggtgtatt cctcaaaact cagtactacg acggacggtt ttttccggtt
      361 gtagactacc tgaggttctt tttctgcctg cgaaaccagc tctgaaaact gagattttgc
      421 agcggcgatt ttccaaacca taatttctcc tgaatgtgac tattttgact gtaaattcag
      481 tttaaaattg gtcaagtaga aaaatagaat tctctccata aaatcgatca gccgtttgaa
      541 tcttgatcca cgttttttta attaaactga gtccggttta aaacaaatcg acaaactcgc
      601 ctctctttca aactttggag ttatgaagag taaatttctt gcaatatctg taatccttgc
      661 tgcttctgtg ctttcgattg cgtatttttc cacaaaggga gaggcttctt tcctgcttct
      721 tgatgcaagc gaacttgctg tcaatcctgc aaagtattca ggagaaaatc tccgtgttcg
      781 aggaaacgta aaaatcgggt ccgtcctgcg tgaagggaga gaagcccgtt ttgttctgga
      841 actgaacgga aaggaagtcc ctgttcattt caccggaaaa aatctgctcc cggatgcgtt
      901 caaggacggt gtcagggcga gagtggacgg acatttcaaa tccggaactc ttgaggcaga
      961 tcatgtggaa gcaaaatgtg catccaagta cgaggcggac tacagcaaat gacactgaat
     1021 gatctcggct cagcagtcat ggtggtttcc tgcggactta cagccttcag tcttttccag
     1081 actgtgtacg gaatgctccg tcacgatgaa agtgccgtca ggctgggtca gacttcgctc
     1141 ctcgcaaatt ttggagtcat ccttctcgca tttgtcattc tgctgactgc cttggccaga
     1201 atggatctta cgaatcatta tgccgtaat
//
LOCUS       DAOQGJ010001587         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_92946_length_1228_cov_2.359761, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001587 DAOQGJ010000000
VERSION     DAOQGJ010001587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92946_length_1228_cov_2.359761"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PK683_19850"
     CDS             complement(<1..331)
                     /locus_tag="PK683_19850"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNH10762.1"
                     /translation="MKKESDIILDGFSGEELFAGQIGLTYRDFLVLPGFIDFNPSDVE
                     LETNLTRNIKIKRPLVSSPMDTVTEASMAIALALMGGIGIIHYNNSMDEQVAHVRKVK
                     RFENGFIT"
     gene            402..>1228
                     /locus_tag="PK683_19855"
     CDS             402..>1228
                     /locus_tag="PK683_19855"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HNH10763.1"
                     /translation="MEKYTIERNVRDMEDVVDRQKRFALFSRFLMNSDIVLDDPKKGQ
                     FRGKIVGFSDDCTNFSASVAGGMFIKGTKLTLNKFVGRYIRIECTVISVQPNSVFVME
                     INSLRMAKKNRAGTRISPPKDMVYANNFHLTRPNADMDEFSVPTFVKITFSDYQNKLS
                     GKFDHIEIDTFKPGLPEKFYLVKSTGKSIYISDTQDLKSYRSTDEEEFLDCTEEFLDD
                     LTDVMKDYRNKKIAAEIIMPVIYLNSTNQAIPIGYVLIQSYTKKIEFDDLMEAKILC"
BASE COUNT          390 a          246 c          232 g          360 t
ORIGIN      
        1 cagtgataaa tccgttttcg aatcttttca ctttcctcac atgggccacc tgttcatcca
       61 ttgaattgtt gtaatggatg atcccgattc cccccatcag tgcaagtgca attgccatgg
      121 aagcttccgt tacagtgtcc atcggtgaac tgacaagagg tcttttgatt tttatatttc
      181 tagtaagatt cgtctccagt tctacatcac taggattaaa atcaataaat cctggaagaa
      241 ccagaaaatc tctgtaagta agtcctatct gacctgcaaa aagctcttct cccgaaaaac
      301 cgtctaatat aatatctgat tcttttttca tagattatcc tgattcacaa ataaaagagt
      361 tgtttttctg cttgcaacat tatttctttg taacctgaat tatggaaaaa tatacgatag
      421 aacgcaatgt tcgggatatg gaagatgtgg tcgacagaca gaagcggttt gctctgtttt
      481 cccggttcct gatgaacagt gatattgtcc ttgatgatcc taagaaaggg caatttcgtg
      541 ggaaaatcgt cggtttttca gatgattgta caaatttttc agcctcagtt gcaggcggaa
      601 tgttcataaa aggaacaaaa ctgactctga acaaatttgt cggcaggtat attcgaattg
      661 aatgcactgt catttcagtt cagccaaatt cagtatttgt aatggaaatc aacagcctga
      721 gaatggccaa aaagaatcgc gcaggcacga gaatttctcc tccgaaagat atggtatatg
      781 caaataattt ccatctgacg agaccgaacg cagatatgga cgaattctct gttcctacat
      841 ttgtaaaaat caccttttcc gactatcaga acaaactgtc cggaaaattt gaccatattg
      901 aaattgatac attcaagcct ggtcttcctg aaaaatttta ccttgtcaaa agcacaggaa
      961 aaagcatcta tatcagcgac actcaggatc tgaaaagcta cagatccaca gatgaggaag
     1021 aattcctgga ctgcacggaa gaatttctgg atgatctgac tgatgtaatg aaagactaca
     1081 gaaataaaaa aatcgcagcc gaaatcatta tgcctgtcat ctatcttaac tccacaaatc
     1141 aggcgatccc tatcggttat gtactgatcc aaagttatac caaaaagatc gagttcgatg
     1201 atctgatgga agcaaagatc ctgtgttt
//
LOCUS       DAOQGJ010001588         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_93091_length_1227_cov_2.507679, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001588 DAOQGJ010000000
VERSION     DAOQGJ010001588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93091_length_1227_cov_2.507679"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PK683_19860"
     CDS             complement(<1..1159)
                     /locus_tag="PK683_19860"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH10764.1"
                     /translation="MKEKKRICSRKLVSWILCFVLAGSFSVSAEEKTGVGFKTHYKKR
                     EHTGRKKFALLVGVKESEKKGFGTLANAEKDIAEIERNLVQKGDFTVKSFNSNPKLKE
                     VTDWMDKFVTDLPEGSVFLFFFSGHGTAAQDGSTLMYLYDSDQSNEVTALKESKLLEK
                     LLKKNEGLPVGKKIDIILFINACRTANDEAIKTISKEYQNREQGANVTRFYSSQIGQR
                     SIDNSDKATNALSLFTFRFVQAINNSAIPKKPDEKESNKPSEQKVSNAEDTSFFGADS
                     TVNGGNGDRKITLVEIKKFMDRVFKNEIQKPEDFIADGMQDFPLVDLNKSYDTLRYIW
                     RSAVMPGWGEWNYGVDYNERISGKISYYTIFVYPVIYVGLIGKTVADFRVYS"
BASE COUNT          330 a          289 c          208 g          400 t
ORIGIN      
        1 tgctgtagac tcgaaagtcg gcgacagtct ttccgatcag tccgacatag attaccggat
       61 atacaaaaat cgtataataa gaaattttac cgctgattct ttcattataa tcaactccat
      121 aattccattc cccccaaccg ggcatcactg cggaacgcca aatatatctc aaggtatcat
      181 acgacttgtt cagatccact aaaggaaagt cctgcattcc atcggcaata aaatcttccg
      241 gtttctggat ttcgttttta aacactctgt ccataaattt cttaatttcc acgagggtga
      301 ttttacggtc tccatttcca ccgtttacgg tagagtctgc tccgaaaaaa gatgtatctt
      361 ctgcattcga taccttctgt tctgagggtt tattggattc tttttcatct ggctttttag
      421 ggatggcaga attattaatt gcctgaacaa aacgaaaagt gaacagactt agagcgtttg
      481 tcgccttatc ggaattatcg atggaccttt ggccgatttg ggaggagtag aatcgggtga
      541 catttgcccc ttgttcacga ttctgatatt ccttgctgat agtttttatt gcttcatcgt
      601 tcgcagtacg gcaggcattg ataaacagaa taatatcaat ttttttacca accggcaagc
      661 cttcattttt cttcaaaagt ttttcgagaa gttttgattc cttcagagcc gtgacctcat
      721 tgctctgatc agaatcatac aagtacatca gagtactccc atcctgagct gcagttccat
      781 gccccgaaaa gaaaaacaga aacacgctgc cttcaggaag atctgtcaca aatttatcca
      841 tccagtcagt cacttccttc aacttagggt tgctattgaa ggattttact gtaaaatccc
      901 ctttctgcac aagattcctt tcaatctcgg caatatcctt ctccgcattg gccaaagtgc
      961 cgaatccctt tttttctgat tccttgactc caacaagaag ggcaaatttc tttctgccag
     1021 tgtgctctct ttttttataa tgcgtcttaa atccgacccc tgttttttcc tcagccgaca
     1081 cagaaaaact ccctgccaat acaaaacata aaatccagga aacaagtttt cgagaacaaa
     1141 ttcttttctt ttctttcatt tgcaattaca actctcccga taatgaagag cattttcacc
     1201 ttccgttttt ttgatctttt tccggga
//
LOCUS       DAOQGJ010001589         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_93184_length_1226_cov_3.495303, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001589 DAOQGJ010000000
VERSION     DAOQGJ010001589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93184_length_1226_cov_3.495303"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1226
                     /locus_tag="PK683_19865"
     CDS             <1..>1226
                     /locus_tag="PK683_19865"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNH10765.1"
                     /translation="LSIYLYPKFSLPKVSFLSFGLFMGIAMSVTAFPVLARILQEKGF
                     ARTQVGAIVITAAAIDDISAWIMLALIIAVIHSGGIIQSFALIGFVLLYAAGMIFVLR
                     PFLNKLGKVYITKEYIGRGVMTFLVTIMMFSALTTEVLGIHALFGAFLAGVIMPSDSK
                     LRMTLRDKFEDFSAVVLLPLFFAYTGLRTQIGLISTTQDLLDTLIIILAAVAGKFGGG
                     MAAARLSGIGWKDSGIIGTLMNTRGLMELVVLNIGYDLGILSPKIFAMMVLMALITTF
                     MTSPVLDLFKRNGKASDQHRPGNFPDLLITFGPPKSGEILFRLAHSIFGRTVHYSALH
                     ISPIPETISLDDPVNLKEENFSLIRDAARKSDVQITPIFKMTNNTSLEIVNTIQKSEY
                     SMVFMGGAKKIFGDNV"
BASE COUNT          301 a          271 c          287 g          367 t
ORIGIN      
        1 ctctttccat atacctttat cctaagttca gtcttcccaa ggtcagtttt ttaagcttcg
       61 gtctttttat gggaatagcg atgagcgtga ctgcatttcc ggtccttgcc cgcattcttc
      121 aggaaaaggg atttgcgaga acccaggtgg gggcgattgt catcactgca gcagccatcg
      181 atgatatttc tgcctggatt atgcttgcac tgatcatcgc ggtcattcat tccggcggaa
      241 tcattcagtc gtttgcactg atcggatttg tactgctgta cgcggcagga atgatttttg
      301 tgcttcgccc ttttctgaac aagctgggaa aagtatatat cacaaaggaa tacatcggaa
      361 gaggtgtgat gacatttctt gtgacaatta tgatgttttc agcgctgaca actgaagttt
      421 tgggaattca tgcacttttc ggagctttcc tggcaggagt cattatgccc tccgattcaa
      481 agctcagaat gactctgaga gacaagtttg aagacttcag cgcagtggtg cttctgcctc
      541 ttttctttgc ctatactgga cttcgcaccc agataggtct gatttccacg actcaggact
      601 tactcgatac actgattatc atccttgcgg ctgttgctgg aaagttcggg ggaggaatgg
      661 ctgcagcaag gctttccgga atcggatgga aggattcagg aatcataggg acacttatga
      721 ataccagggg tctgatggag cttgtggtgc tgaacatagg atatgatctt ggaattcttt
      781 cccctaagat ttttgcaatg atggttctga tggcattgat caccaccttt atgacaagtc
      841 ctgtgctgga tcttttcaag agaaacggca aggcatccga tcagcacaga ccgggcaatt
      901 ttcctgacct gctcattact ttcggtcctc ccaagtccgg cgagattctg ttccgtctgg
      961 ctcattccat tttcggaagg actgtgcatt attctgctct tcatatttcc ccgattcctg
     1021 aaacgatttc tctggatgat cctgtgaatc tgaaggagga aaatttctca ctcatacggg
     1081 acgcagcaag gaagtccgat gttcagatca ctccaatttt caaaatgacc aacaatactt
     1141 ctctcgaaat agtaaatact atacagaaat cagaatattc gatggtattc atgggcggag
     1201 ccaagaaaat cttcggagac aatgtg
//
LOCUS       DAOQGJ010001590         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_93528_length_1224_cov_2.298546, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001590 DAOQGJ010000000
VERSION     DAOQGJ010001590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93528_length_1224_cov_2.298546"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK683_19870"
     CDS             complement(<1..282)
                     /locus_tag="PK683_19870"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNH10766.1"
                     /translation="MRKIIILLFIITVQDCSSYIPRKEISEKFFGKKFQIIAHRGMRN
                     YAPENSMSAFRMAHSRGFPFELDVMFCRTGELVVFHDETVDRVTGKKGKV"
     gene            complement(279..875)
                     /locus_tag="PK683_19875"
     CDS             complement(279..875)
                     /locus_tag="PK683_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10767.1"
                     /translation="MRIYAVLLFLSFCIPGRDDQVILRKKNPMPNPSVSSAGFPMNSS
                     SIETVHPTKTVFRDASIHAGRTPLSQKAAGPRSTEALSFINRSVIPPKDFLNTNLSDW
                     NLEKYEIVKITGKSKGCRNSVNVPIIFHSDGRSRPILLNFSFALKTFYEELNELTEMN
                     SVEALFDSRLDEERQSYFFGAYSKKCFVFHSFGLRKKK"
     gene            complement(872..>1224)
                     /locus_tag="PK683_19880"
     CDS             complement(872..>1224)
                     /locus_tag="PK683_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNH10768.1"
                     /translation="SQSYADIKGMQTVGKLSGETCSESWFWLVYLGDESFETAVHKAV
                     ENRADLLFNVQTDYSLEQILFGFLRLKKCTRVTGIGVKFGSVPEKLELGTVPEKSAES
                     SSEKTSDKEIKKKK"
BASE COUNT          341 a          279 c          242 g          362 t
ORIGIN      
        1 cactttcccc ttttttccgg tcaccctgtc cacagtctcg tcatgaaaaa caacaagttc
       61 acctgttctg cagaacatca catccagttc aaaaggaaat cctctcgaat gagccattcg
      121 gaatgcagac atggaatttt caggagcata gtttctcatt cccctgtgag caataatctg
      181 aaattttttc ccgaaaaatt tttccgaaat ttctttcctg ggaatatagg aagaacagtc
      241 ctgaactgta atgataaaaa gaaggattat aatttttctc attttttttt cctcaacccg
      301 aatgaatgga acacaaaaca tttttttgaa taggcaccga aaaagtaact ctgcctctcc
      361 tcatcgagtc tgctgtcaaa cagtgcttcc actgaattca tttccgtaag ttcattcagt
      421 tcttcataaa aggtcttgag cgcaaaactg aaattcagga gaatcggtct ggacctgccg
      481 tccgaatgaa atatgatcgg cacatttact gaattcctgc agcccttgct tttgcctgta
      541 atttttacga tctcatattt ttcgagattc cagtcggaga gatttgtatt caggaaatcc
      601 ttaggaggaa tcactgaccg gttgataaag gagagagcct ccgttgaacg aggacctgct
      661 gctttctgag aaaggggtgt ccttccggca tgaatggaag catctctgaa tacagttttc
      721 gtcggatgga ctgtttcaat ggaagaggaa ttcatcggaa atcctgcaga ggaaaccgaa
      781 ggattcggca taggattttt ctttcgaagt atcacctgat cgtctctgcc gggaatacag
      841 aaggaaagaa aaagaagaac tgcataaatt ctcatttctt ctttttgatt tccttatccg
      901 aagttttctc cgaggaagat tcagctgatt tttcgggaac ggttccaagc tccagttttt
      961 ccggaactga gccgaatttt actccgatcc cggtcactct tgtgcacttt ttcaatcgga
     1021 gaaatccgaa aagaatctgc tccagactgt agtctgtctg cacattaaac agcaggtcgg
     1081 ccctgttctc tacggcctta tggacagcag tctcgaaact ttcatctccc agatatacga
     1141 gccagaacca gctttcagaa caggtctcac cggaaagttt ccctacagtc tgcatccctt
     1201 taatatctgc ataactttga gaat
//
LOCUS       DAOQGJ010001591         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_93906_length_1221_cov_3.481132, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001591 DAOQGJ010000000
VERSION     DAOQGJ010001591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93906_length_1221_cov_3.481132"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..732
                     /locus_tag="PK683_19885"
     CDS             142..732
                     /locus_tag="PK683_19885"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10769.1"
                     /translation="MKKLQTIITLIILLVSIVCCKNRQAEMQEDAENLVKLIGTRDMK
                     ASLARALSLIEKYPDQTDFYVVTASIYTSMKDDANTVKYYEKALSIDPKSSDVLYQAG
                     FYFFSKNDCKSSLKYLEQASVEKSAYESAKIHAEVLYVLGFCSHAEKNRQNAEKYFRK
                     FQETVGDYDYNDKSVRNMINFTAATLKAEYSETPKK"
     gene            975..>1221
                     /locus_tag="PK683_19890"
     CDS             975..>1221
                     /locus_tag="PK683_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10770.1"
                     /translation="MGIIEASSDFISEEMRFNNNELLEKFMKWQDHFKANTVTADEAV
                     KNVKNGNRIFVSGNATLPTSLTDALVKRGNELEGVEMN"
BASE COUNT          410 a          214 c          259 g          338 t
ORIGIN      
        1 ggatatggtc aattatatga atgaaaagtc caaggaaatg ggacttctca gacccggaga
       61 tacgatagtc attctgtccg gatctccagg cggacacgca aattctgtcg actttgtgca
      121 gatttataag atcagataat tatgaaaaaa ctccagacaa taataacgct tattattctt
      181 cttgtttcta ttgtatgctg caaaaacaga caggctgaaa tgcaggaaga tgctgaaaat
      241 ctggtcaagc tgatcggaac aagagatatg aaagcctcat tggcaagagc attgagtctg
      301 attgaaaagt acccggatca gactgatttt tatgttgtga cagcaagcat ctatacctca
      361 atgaaagacg atgccaacac tgtgaaatat tacgaaaaag ctctttccat tgatcctaag
      421 tcttcagatg tactttatca ggccggtttt tatttcttct ccaaaaatga ctgcaaatcc
      481 tctttgaagt atctggaaca ggcttctgtc gaaaaatctg catatgaatc ggcgaaaatt
      541 catgcggaag tgctttatgt tctgggcttc tgcagccatg cggaaaagaa caggcagaat
      601 gcagaaaagt atttccgaaa atttcaggaa acagtcggtg actatgatta taatgataaa
      661 agtgtgcgaa atatgattaa tttcactgca gcgactctaa aagcagaata ttcggaaacc
      721 cccaaaaaat aaaatttcag aaggatggat ttgcctgtga acggtccgtc ttttcgctga
      781 ttatgatgaa aatcatatca gtcagaatgt aaaaaaactg acaaaattta cggatgattt
      841 gttattgtac agctgaatga agcctgaaaa gacagcagat tttaaataaa ccggacaaag
      901 cttttgtgca gccgcagttt tagaatcctt ctgtcaggct gcacccgatc cttgctgaaa
      961 aacaggaaag gcttttgggt atcattgaag cttcttcaga ctttatttct gaagaaatgc
     1021 gttttaataa taatgaatta ctggagaaat ttatgaaatg gcaggatcat tttaaggcaa
     1081 atactgttac tgcggatgag gcagtaaaaa acgtgaaaaa tggaaaccgt atttttgttt
     1141 ccggaaatgc aactcttcca acttctctga cagatgcgct tgtcaagaga ggaaacgaac
     1201 tggaaggagt cgagatgaac c
//
LOCUS       DAOQGJ010001592         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_93976_length_1221_cov_2.222127, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001592 DAOQGJ010000000
VERSION     DAOQGJ010001592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93976_length_1221_cov_2.222127"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK683_19895"
     CDS             <1..480
                     /locus_tag="PK683_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10771.1"
                     /translation="LRDLKKIITVFIKKIESERSKLHVLATDEAKGIYSFLIYENPEN
                     TDPRFIEPLADFAVWARDFFQQICAERNLPYGSRMGMHTGKFTDYKYEPVNSLLRIFK
                     SEAVFEKGQTAQRESVTDEIQVTHEVYLLLKERYVLVRRHNISGPEEESFYFLKGKK"
     gene            480..1007
                     /locus_tag="PK683_19900"
     CDS             480..1007
                     /locus_tag="PK683_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10772.1"
                     /translation="MALSAGFSEIVTFILTVFFLALLVWMWMKRSKWLLPIGAVLFMF
                     LGFGTYSVYKYLKTGFDDGPFIPEKIAELNDSFVLRESIPWKNGRLSVYESPNHPLTV
                     SYEEKDGRKKFWVQKLSLKELPGFKIRRISSLYISRNIFFQEELCFTACWDFGCERGR
                     AVLSSAGIQEYYLSW"
     gene            1009..>1221
                     /locus_tag="PK683_19905"
     CDS             1009..>1221
                     /locus_tag="PK683_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10773.1"
                     /translation="MSKLKSSDLVSSFQKRDISSYRKEEHEKMLSSYSKEQALESAEK
                     ILKDIALKENDLLNRGIRSAVIGAIYG"
BASE COUNT          363 a          211 c          289 g          358 t
ORIGIN      
        1 cttcgggatt taaaaaaaat cattacggta ttcattaaaa agatagaatc agagaggtca
       61 aagcttcatg tgctggctac agatgaggcc aaggggattt attcgttcct gatttatgaa
      121 aatcctgaaa atactgatcc gagattcatt gagccgcttg cagattttgc cgtctgggca
      181 agagactttt tccagcagat ctgtgccgaa agaaatctgc cttacggttc gagaatggga
      241 atgcatactg gaaaatttac cgattataaa tatgaaccgg tcaattccct tctgagaata
      301 ttcaagtcag aggcagtttt tgaaaaggga cagactgctc agagggaaag tgtgacagat
      361 gaaattcagg tgactcatga ggtctatctt ctgctgaagg aaagatatgt tctggtaaga
      421 aggcacaata tttccgggcc ggaagaagag tctttttatt ttctgaaggg aaagaaataa
      481 tggctctctc tgcaggattt tccgagatcg ttacatttat acttacagtt ttttttctgg
      541 ccctgcttgt atggatgtgg atgaaaagat ccaagtggct tctcccgata ggggctgttc
      601 tttttatgtt tctaggtttt ggaacctatt ctgtgtacaa atatttaaag accggatttg
      661 atgacggtcc ttttattccg gaaaaaattg cggagctgaa tgactccttt gtgctgaggg
      721 aaagtattcc ttggaaaaat ggaagacttt ccgtttatga gtctccaaat catccattga
      781 ctgtctctta cgaggaaaag gacggaagaa aaaaattctg ggtacagaag ctttcattga
      841 aagaactgcc cgggttcaaa attcgaagaa tcagcagtct ttatatttca cggaatattt
      901 tttttcagga agagctttgt ttcacagcgt gctgggattt tggatgcgag agaggcagag
      961 ctgtcctttc gtctgcaggc attcaggaat attatctgag ctggtaaaat gtcaaaattg
     1021 aaatcctcag atctggtatc ctcttttcag aaaagggata tcagcagtta caggaaggaa
     1081 gagcatgaaa aaatgctttc ttcctattcc aaagaacagg ctctggagtc cgcagagaaa
     1141 attctgaaag atatcgcctt gaaagaaaat gatcttctga atcgcgggat cagatcagct
     1201 gtcatcggcg caatatacgg g
//
LOCUS       DAOQGJ010001593         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_93980_length_1221_cov_2.184391, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001593 DAOQGJ010000000
VERSION     DAOQGJ010001593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93980_length_1221_cov_2.184391"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PK683_19910"
     CDS             <1..587
                     /locus_tag="PK683_19910"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH10774.1"
                     /translation="MVLGDESRTVFGKEHITEHLNEFRFRAGLDTFVQVNPYQAPFLY
                     EKAVELIPESSRVAEAYSGIGTLTHMIARKAKSVTAVELNGKSVEAGRKAALENGMKN
                     IRFIQGDAAEILGSAGRADCIVLDPPRTGLHFSMIKKLIKYNAKSIIYISCDPVTLER
                     DAWNLRNAYRLLDIQPVDMFPHTNHIESVACFSS"
     gene            658..1185
                     /locus_tag="PK683_19915"
     CDS             658..1185
                     /locus_tag="PK683_19915"
                     /inference="COORDINATES: protein motif:HMM:NF022766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HNH10775.1"
                     /translation="MKKAFTVCAVICIFLSFDCRQKEKKNFQNREISEPDLPVQKKPI
                     SIVSGSRVYVPAYARAFSYRNEQTVSLVTTLAVHNTDMENPIFLRKIEYYSEGGRLLK
                     EFMKEPKILGPMATAVYPLVPEKEGAGIGANAVVEWVSEHKVSRPVIEAIMISEGTLG
                     ISFISPGYSVKKITE"
BASE COUNT          348 a          248 c          287 g          338 t
ORIGIN      
        1 atatggtgct cggtgatgaa tcaagaactg tttttggaaa agagcatatt acggagcatc
       61 tgaatgaatt cagatttcgg gccggactgg atacatttgt tcaggtcaat ccgtaccagg
      121 caccttttct gtatgaaaag gctgtggagc ttattcctga aagcagcaga gtcgcggaag
      181 cctattccgg gataggaact ctgactcata tgatagcccg gaaagcaaag tcggtcactg
      241 cagtggaact gaacgggaag tcagtggaag ccggaagaaa ggcagctctc gaaaacggga
      301 tgaaaaatat ccggttcatt cagggggatg ctgctgaaat cctgggatct gcaggacggg
      361 cagactgcat cgtactggat ccgccgagaa ccggactgca tttcagcatg attaaaaaac
      421 tgataaaata taatgcaaaa agcattattt atatatcctg tgacccggtc acgcttgaaa
      481 gagacgcctg gaatcttcgg aatgcttaca gacttctgga tatacagcct gtggatatgt
      541 ttccgcatac gaatcatatt gaatctgttg cctgtttcag ttcctgagtt taatgcctat
      601 tctgtttttt cggaaaataa attgacttca attctttctg cagtacaaag gattattatg
      661 aaaaaagcct tcactgtctg tgctgttata tgcatattcc tgtccttcga ctgccgtcag
      721 aaagaaaaaa agaattttca gaatcgtgag atttcggaac ctgatcttcc tgttcagaaa
      781 aagccgatca gcattgtttc cggcagccgt gtatacgttc ctgcatatgc cagggcattc
      841 tcgtacagaa atgagcagac agtttctctg gtgacaactc tggctgttca caataccgat
      901 atggagaatc ccatttttct gagaaaaata gaatactatt ccgagggcgg aaggcttctg
      961 aaggaattta tgaaagaacc taaaattctc gggcctatgg caactgctgt atatcctctt
     1021 gttcccgaaa aggaaggtgc aggcatcggt gcaaatgccg tagtggagtg ggtgtccgaa
     1081 cacaaggtat cccgtccagt cattgaagcc ataatgatct ccgaaggcac tctcgggatt
     1141 tcattcatca gtccaggtta ttctgttaaa aaaattacgg aataatgatt atttgtgtcc
     1201 gatttttctc acctgcgatt t
//
LOCUS       DAOQGJ010001594         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94076_length_1220_cov_3.247210, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001594 DAOQGJ010000000
VERSION     DAOQGJ010001594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94076_length_1220_cov_3.247210"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1220
                     /locus_tag="PK683_19920"
     CDS             <1..>1220
                     /locus_tag="PK683_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH10776.1"
                     /translation="KNTAVLYKNTRINIVDTPGHADFAGEVERVLNMADCSLLLVDAF
                     DGPMPQTRFVLGKSLQIGHKPIVVVNKVDRDGARPNEVVNMVFDLFSDLNATDEQMDF
                     PIIFSSAKQGWSVYDMKDSPGKNLEPLLDTVLKHVPLAKGDPKKPLQFQVTSLDYNDY
                     VGRIAIGKIYEGSIKKNLDVTTVQMLSGKNVNNRITRLYGFEGLRRHEIESAEAGDIV
                     AIAGIPDVFIGDTVCDLGNPLPRPAISIDEPTVSMFFMVNNSPFVGKEGKYVTTRNLK
                     ERLDKELQTNVAMRLEETDDKDRFKILGRGELHLSILIETMRREGYELQVSRPEVIFK
                     YGKEGEKLEPMETLVMDLPDSYTGSIIAELNRRKGEMTAMNAHTSGMTRVEYNIPTRG
                     LIGFRGFFLTETRG"
BASE COUNT          369 a          235 c          310 g          306 t
ORIGIN      
        1 aaagaacaca gctgtattgt ataaaaatac cagaatcaat atagtggaca ctccgggtca
       61 cgcggatttt gccggtgagg tggagagggt tctgaatatg gcagactgct ctcttcttct
      121 tgtggatgca tttgacggtc ctatgcctca gaccagattt gttctcggaa aatcacttca
      181 gatcggtcat aagccgattg tggtagtcaa caaggtggac agagacggtg caagaccgaa
      241 tgaagtcgtc aatatggtct ttgatctttt ttctgatttg aatgctacag atgaacagat
      301 ggactttcct attattttct cttctgcaaa acagggctgg tcggtatatg atatgaagga
      361 ttctcccgga aaaaatctgg aacctcttct ggacacagtg ctgaagcacg ttccgcttgc
      421 gaaaggtgat ccaaagaaac ctcttcagtt ccaggtcact tcattagatt ataatgatta
      481 tgtaggaaga attgcgatcg gcaagattta tgagggttcc attaaaaaga acctggatgt
      541 gactacagtt cagatgctca gcggaaagaa tgtaaacaac agaatcacaa ggctctacgg
      601 atttgaaggc ctgagaagac atgagattga atctgctgag gccggggata tcgtagcgat
      661 tgcaggtatt ccggatgtgt tcatcggaga tacagtctgt gatctgggaa atccgctccc
      721 gcgtcctgca atcagtattg atgagccgac agtatccatg ttctttatgg tgaacaattc
      781 ccctttcgtc ggaaaggaag gaaaatatgt gactaccaga aatctgaaag agagactgga
      841 caaggaactg cagacgaatg tggctatgcg tctggaagaa acagacgaca aggacagatt
      901 caagatcctg ggaagagggg aactccatct ttccattctc attgaaacaa tgagaagaga
      961 aggttacgaa ctccaggttt cgagacccga ggtcattttt aaatatggaa aggaaggcga
     1021 aaagctggaa ccgatggaaa cattggttat ggatcttcct gacagttata ccggttccat
     1081 catcgctgaa ctgaacagaa gaaaaggcga gatgactgca atgaatgcac acacatccgg
     1141 aatgaccaga gtggaataca atatccctac cagaggtctg atcggattca gaggattttt
     1201 cctgactgag actaggggag
//
LOCUS       DAOQGJ010001595         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94242_length_1219_cov_2.739691, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001595 DAOQGJ010000000
VERSION     DAOQGJ010001595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94242_length_1219_cov_2.739691"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..770)
                     /locus_tag="PK683_19925"
     CDS             complement(60..770)
                     /locus_tag="PK683_19925"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNH10777.1"
                     /translation="MILRILSAIFIAGIMMECSSKPVTLATATRKVDEKSAEYKSRST
                     APDSPPGSCFIRKKTPAVYNTVSEKVLVKPAETKTVDVPEVTETVEERIIDKEGYTYW
                     VTNETNEIWYQEEVPTTYKIVKKQIVKSPAERKTVEIPAEYRTIDKQAVVKEESYDWQ
                     EVLCGNNATKETVKSLQDKLKSAGFSPGNTDGNLDEDTMKALNEYQSKNRLPVDKNNY
                     INMESLKKLGVKVQPKQS"
     gene            complement(787..>1219)
                     /locus_tag="PK683_19930"
     CDS             complement(787..>1219)
                     /locus_tag="PK683_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10778.1"
                     /translation="LNNVLNDASAPDFENRLNQLAESDSFKKSFLSFLQTAKEKRVPE
                     NCRGTKIPAELIEECTDTHILLKLDTKENRNKYYQMHLNGNHFEFRLQAYDYFSGECP
                     EYASAKNTAEERLKRKDTVPAETEKAEKLLDHFRSCGSEKK"
BASE COUNT          262 a          286 c          229 g          442 t
ORIGIN      
        1 gcgttcctgc cagaaacgcc ccacctgcgg ccgttaggcg taaggtgagt ttgtaaaaat
       61 caagactgtt taggctgaac ctttactccc agttttttta atgattccat attgatataa
      121 ttgttcttat ccacaggaag ccgatttttg gactggtact cgttcagtgc cttcattgta
      181 tcctcatcca ggtttccgtc agtatttcca ggactgaatc ctgcactctt aagtttatcc
      241 tgcagacttt tcactgtttc ctttgtcgca ttgtttccgc acaggacttc ctgccagtca
      301 taggattctt ccttcacgac tgcctgcttg tcaattgtcc tgtactctgc gggaatttcc
      361 actgtcttcc tttctgccgg cgatttgact atctgttttt ttacaatttt gtaggtggtc
      421 ggaacctctt cctggtacca gatttcattt gtttcatttg tcacccagta tgtgtagcct
      481 tccttgtcta tgatccgttc ttccacagtc tcagtcactt caggcacatc tacagtcttt
      541 gtttcagcag gctttacaag caccttctct gatactgtgt tgtacactgc cggagttttt
      601 ttccggataa agcaggaccc tggaggtgag tcaggagctg ttgatcggga tttatattcg
      661 gcagattttt catccacttt tcttgtcgct gttgcaagag ttacaggctt tgaactgcac
      721 tccatcatta ttcctgcaat aaatattgca gaaaggattc ttaaaatcat taatttctcc
      781 tcatttttat ttcttttccg aaccgcagga tctgaaatga tccagcagtt tttccgcttt
      841 ttccgtttct gccggaactg tgtcctttct tttcagtctt tcttctgcag tattttttgc
      901 agaagcatat tctgggcact cacctgaaaa ataatcatag gcctgaagtc tgaattcaaa
      961 atgatttccg ttcagatgca tctgatagta tttgttcctg ttttcttttg tgtccagttt
     1021 cagcagaata tgagtatcag tgcattcttc tatcagctct gcagggattt ttgtccctct
     1081 gcagttttca ggcacccttt tttcctttgc agtctgcaaa aaagacagaa aacttttttt
     1141 aaatgaatcc gactccgcca gctgattcag cctgttttca aagtcaggag cggaggcatc
     1201 attcagtaca ttattcaaa
//
LOCUS       DAOQGJ010001596         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94243_length_1219_cov_2.736254, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001596 DAOQGJ010000000
VERSION     DAOQGJ010001596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94243_length_1219_cov_2.736254"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK683_19935"
     CDS             complement(<1..233)
                     /locus_tag="PK683_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004761594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH10779.1"
                     /translation="MIRIQNLNKIYRGFISPKERILSALSMGFFRGRREYSALHNISF
                     ELKSGEICGIIGRNGAGKSTLLKVLSGVSPYDSG"
     gene            complement(230..973)
                     /locus_tag="PK683_19940"
     CDS             complement(230..973)
                     /locus_tag="PK683_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH10780.1"
                     /translation="MSLRSLLILVKRDYAVQYSGAVFGIAWAVLQSFAQIGIFLLASW
                     LLVRSEKSTASAAYSLTGLLFWIPIQDMILKGNSILSENRNLLKKSGIKMEYFLMIPM
                     LQMAVQSALIGVPVFIFLSVYSEISLMMIPAAYSVFLITAAVIYPFVQYLSLSNVILK
                     DISPAVRIFLQGAFWSLPVLYVPSGSFQRIAQFHPLHFPLSVFRFLLTDESFPGTQSG
                     IIFFCFCIISVLVFFISSLRYRKSIMDHL"
     gene            complement(983..>1219)
                     /locus_tag="PK683_19945"
     CDS             complement(983..>1219)
                     /locus_tag="PK683_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate decarboxylase"
                     /protein_id="HNH10781.1"
                     /translation="VDDSLETVKSEADFLWTIFTRFEPAGDMYGKTVIERNHIGFEDS
                     IVFDCRMKDWYPSVLSPSPEIVEQVSRRFGKLLD"
BASE COUNT          383 a          263 c          251 g          322 t
ORIGIN      
        1 ccggagtcgt aaggactgac tcctgaaaga accttcagaa gtgtcgattt gcccgctccg
       61 tttcttccta tgattccgca gatttccccg ctctttaatt caaagctgat attgtgaagt
      121 gcagaatatt ctcttctgcc tctgaagaaa cccatactca aggcagacag gattctttct
      181 ttcggtgaaa taaatcctct gtatatcttg ttcagatttt gaatcctgat cataaatgat
      241 ccatgatgct ctttctgtaa cgaagactcg aaatgaaaaa tacaagaacc gaaattatgc
      301 agaagcagaa aaagattatt cctgactgag ttccaggaaa agattcatcc gtcaggagga
      361 atctgaagac tgaaagagga aaatgaagag gatgaaattg agcgatcctt tgaaagcttc
      421 ctgaagggac gtaaagaaca gggagggacc agaacgcccc ctgaagaaaa attctgactg
      481 ccggactgat atccttgaga atcacatttg aaagacttaa atactgaaca aacggataaa
      541 ttacggcggc agttataagg aagactgagt atgccgcagg gatcatcatc agactgattt
      601 cagaatagac tgagaggaaa ataaatacag gcacacctat cagtgccgac tgaaccgcca
      661 tctgcagcat aggaatcatt aaaaaatatt ccattttgat tcctgatttt ttcagcagat
      721 ttctgttttc agaaaggatg gaatttccct tcagtatcat atcctgaatc ggaatccaga
      781 ataaaagtcc tgtcaaagag taggccgctg aagccgtgct tttttcggac ctgactagaa
      841 gccaggaagc cagaaggaaa attcctatct gagcaaaact ctggagtact gcccaggcaa
      901 tcccaaagac tgccccggaa tactgaacag cataatctct ttttaccaga atcagcagtg
      961 accgcagact catggattta tttcagtcca gaagttttcc gaatcttctg ctgacctgtt
     1021 caacaatttc aggggagggt gaaagtacag acggatacca gtccttcatt ctgcagtcaa
     1081 agacaatgct gtcttcaaac ccaatgtgat ttctttcaat gactgtcttt ccatacatat
     1141 ctcctgccgg ttcaaagcgt gtgaatattg tccatagaaa atcagcttca gattttaccg
     1201 tttccagaga gtcgtctac
//
LOCUS       DAOQGJ010001597         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94260_length_1219_cov_2.506014, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001597 DAOQGJ010000000
VERSION     DAOQGJ010001597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94260_length_1219_cov_2.506014"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PK683_19950"
     CDS             complement(<1..880)
                     /locus_tag="PK683_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10782.1"
                     /translation="MKLWKSYGKVFLLFIAFCILKNPKEEPKTKEEPKKTVRVSEEKK
                     AKFSSSDLVLSKLDVLPKKRGLRAEHYPSSNERRLDLFIPYVENLGGGYVGVGTDQNL
                     SLIAKARSEYAYLMDFDMDIVRVNKMHIFLLKHSETSEIFMERWKRQSKAKTKELIAK
                     EGGTETDDLLKGLEMGHVPYLGVPERFAELSFMNKKFGFVTFTSSKEEYDFLRELALS
                     GRLNAVPGNLLGDTTIGEISAVAQKISVPIRVFYTSNAEEYFSFPKEYRENLKKIHTD
                     EKGVLVRTVSAGTKNGW"
     gene            989..>1219
                     /locus_tag="PK683_19955"
     CDS             989..>1219
                     /locus_tag="PK683_19955"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNH10783.1"
                     /translation="MKKNPFPEIFSIFGLLSVALGAFAAHGLRQILRPEEITVFETGV
                     RYMFFHSVAGLSVSVHSSESGLKKYSQILFTAG"
BASE COUNT          297 a          307 c          233 g          382 t
ORIGIN      
        1 cccatccgtt cttcgttcct gcactgactg ttctgacgag aactcctttt tcatccgtat
       61 gaattttctt cagattttct ctgtactcct tgggaaagga aaagtattcc tccgcattgg
      121 atgtatagaa cacacggatc ggaacggata ttttctgcgc cacagcagag atctctccta
      181 tggttgtgtc tcccagcaga ttgcccggaa ctgcattcag tcttccgctc agtgcaagct
      241 ctcttaagaa atcatattct tcctttgaag atgtgaatgt gacaaatccg aattttttat
      301 tcatgaagga aagctctgca aacctttcag gcactcccag ataaggtaca tgccccattt
      361 cgaggccctt cagcagatcg tccgtttccg ttccaccttc ctttgcaatc agctccttag
      421 tctttgcctt gctctgtctt ttccatcttt ccataaaaat ctctgatgtc tcagaatgct
      481 tcagaagaaa aatatgcatt ttattcacgc gcacaatgtc catatcaaag tccatcagat
      541 aggcatactc ggaacgcgcc tttgcaatga gactcagatt ctgatccgtc cctactccta
      601 catatccccc gcccagattt tccacatagg gaataaacag atccagtctt ctctcattgg
      661 aagacggata atgctctgcc cggagccctc tttttttagg gagcacatcc agttttgaaa
      721 gcaccagatc agaagacgaa aacttggctt ttttttcttc cgaaacccgc actgtttttt
      781 tcggttcttc ctttgtttta ggctcttcct tcggattctt taaaatgcag aatgcaatga
      841 agaggagaaa gacttttccg tatgatttcc ataatttcat acttttattt tcggcagaag
      901 gagattccaa attcagagca gatcggacgg ataagggaaa actgtattga cattctctgt
      961 aaacgggaca gatttgcagt cgcacactat gaaaaagaat ccttttccag aaatattctc
     1021 cattttcgga ctgctgtctg tggctctcgg tgcatttgct gctcacggac tccgtcagat
     1081 tctgcgtcct gaggaaatca ctgtatttga gacaggcgtc cggtacatgt tctttcattc
     1141 ggtcgcaggg ctttccgtct ccgtgcattc ctctgaatcc ggtctgaaaa aatattccca
     1201 gattctcttt accgcaggt
//
LOCUS       DAOQGJ010001598         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94380_length_1218_cov_2.908856, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001598 DAOQGJ010000000
VERSION     DAOQGJ010001598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94380_length_1218_cov_2.908856"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..705
                     /gene="bioD"
                     /locus_tag="PK683_19960"
     CDS             58..705
                     /gene="bioD"
                     /locus_tag="PK683_19960"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNH10784.1"
                     /translation="MIFIAGTGTDVGKTFLSSLILSKYGKELNLAYWKPVQTGTEKDS
                     DEVRKISSVSIIPSVYEFTFPASPHYSAELESREIDFEFLLDEMKQKKSEKIISEGAG
                     GILVPLNRRFLFADLLERLRIPVLICASSVLGTVNHTLLTLQNLDRRNIPVLGFFFCG
                     ADTEITEDSAFTIEKFSGVKYLGRTDFPNRSLAEKEFRQYADENFDHDFIIKDVL"
     gene            702..>1218
                     /locus_tag="PK683_19965"
     CDS             702..>1218
                     /locus_tag="PK683_19965"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH10785.1"
                     /translation="MKIWRPYSVQKTDRMLPTAVSAENEFIYDENGNEYVDAISTWWT
                     SIHGHGRKEIYMPVTEALQKLDHSVLASFANRPSELLTEKLNEFTKGIFKRVHYTDNG
                     SCAVEAAVKIAVQHFQIKKQKTKKKFIRFSHSYHGDTVGAMSVSGSMVFTDTYSSLLF
                     ETEEIPSPDCFR"
BASE COUNT          378 a          245 c          267 g          328 t
ORIGIN      
        1 gattcagaaa tacaaatgga gttgtgctga attgcgaaga cagaaatctg tcagtgtatg
       61 atatttattg caggcactgg aacggatgta ggcaaaactt ttctttccag tctgattctc
      121 tccaagtatg ggaaagaact gaatcttgcc tactggaaac ctgttcagac aggaacagaa
      181 aaggactctg atgaggtgag aaagatttcc tcagtcagca taattccatc cgtatatgaa
      241 tttacttttc ctgcatcccc tcattattct gcagaactgg aaagcagaga gattgatttt
      301 gaatttcttc ttgatgagat gaagcagaaa aaatcagaga agatcatttc agaaggagca
      361 ggaggaattc ttgtcccgct caacagaaga tttttattcg cagatcttct ggaaagactt
      421 cgaataccgg tgctgatctg cgcctcttcg gttctcggaa cagtcaatca tacccttctt
      481 acacttcaga atcttgacag aaggaatatc cctgttctcg gttttttctt ctgcggagcg
      541 gatactgaaa tcacagagga cagtgcattt acaatcgaga aattttcggg agtgaagtat
      601 ctgggaagaa cagattttcc caatcgcagt cttgctgaaa aagaattcag acagtatgcc
      661 gatgaaaatt ttgatcatga ttttatcatt aaggatgttt tatgaaaatc tggagaccct
      721 actctgttca gaaaacggac agaatgcttc caactgcagt cagtgctgaa aatgagttca
      781 tttatgatga aaacggaaat gaatatgtag atgccatatc cacctggtgg acaagcattc
      841 acggacacgg gagaaaagaa atctatatgc ctgtgacaga ggcactgcag aaactggatc
      901 attcggtgct ggcctcattt gcaaacagac cgagcgaact tctgactgaa aagctcaatg
      961 agttcacaaa gggaattttc aaaagggttc attatacgga caacggctcc tgtgcagtcg
     1021 aggcggcagt caaaattgca gtccagcatt tccagataaa aaaacagaag actaaaaaaa
     1081 aattcatccg cttttctcac tcctatcacg gggatacagt cggtgcaatg tccgtcagcg
     1141 gctcaatggt atttacagac acatactcca gtctgctttt cgagacagag gagattcctt
     1201 ctcctgactg cttcagat
//
LOCUS       DAOQGJ010001599         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94435_length_1218_cov_2.109200, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001599 DAOQGJ010000000
VERSION     DAOQGJ010001599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94435_length_1218_cov_2.109200"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PK683_19970"
     CDS             complement(<1..1110)
                     /locus_tag="PK683_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNH10786.1"
                     /translation="MKAIFSILKSFFGLFVKLFPYFFKAALIGSIAGGVFFTGGAYVV
                     WVKEKSRVKANLDKYKSEVTNYYDSTIVKPVKILDKNGELIGEFFRKNYKPIRTDNLK
                     DHATLVWALLSSEDREFYRHSGINYKALGRAFFINIIQFKLSQGGSTITQQLAKLSLA
                     LGERNIFNKITEAFCTYYIENQYDKETILTMYMNQIFMGEGNTGLEEAARYYYNKSAA
                     HVSPAEAALLIGVIPAPSVYNPVKNLKIAMDRQKRILQDMGKQKHLHFDPDKIDKQYD
                     EKIDKYIAKFTENYKVKEIKSADGKVKFASEIGKSGYDRDFRVNLAPDFNDSIRTYIL
                     EKFTSEELERKSITVHTTLDREKQAAAQNFLQGGVD"
BASE COUNT          303 a          273 c          236 g          406 t
ORIGIN      
        1 atccaccccg ccctgaagaa aattctgagc ggccgcctgc ttttccctgt cgagagtcgt
       61 atgaaccgtg atgctttttc tttcaagctc ctcgcttgta aatttttcta atatataggt
      121 tcttatcgaa tcgttgaaat caggagcaag attcactctg aaatctctgt catatcctga
      181 cttgccgatt tcgcttgcaa atttcacttt gccgtctgca gacttgattt ccttcacttt
      241 gtaattctca gtgaattttg caatatactt gtctatcttc tcatcatact gcttgtctat
      301 cttgtcagga tcaaaatgaa gatgtttctg ttttcccata tcctgaagaa ttcttttctg
      361 tctgtccatt gcgatcttca gattcttgac cggattgtac acggaaggag caggaatgac
      421 tccgatcaga agggctgctt ccgcaggact gacatgcgcc gctgacttgt tgtaataata
      481 tcttgccgct tcttcaagtc cagtatttcc ctcacccatg aatatctgat tcatgtacat
      541 ggtgagaatg gtttccttgt catactggtt ttctatataa taagtgcaga atgcttccgt
      601 gattttattg aatatgtttc tttctcctag ggcaagactg agttttgcaa gctgctgagt
      661 aatagtgctt cccccctgcg aaagcttgaa ctgaatgata ttgatgaaaa atgctcttcc
      721 cagtgccttg taattgatcc cggagtgtct gtagaactcc ctgtcctcag atgaaaggag
      781 cgcccatacc agtgtcgcat ggtctttaag attgtccgtc ctgatcggct tgtagttttt
      841 tctgaaaaat tcaccgatca gttctccgtt cttgtccagg attttcacag gctttacgat
      901 tgtggaatcg taataattcg tcacctcgga tttatacttg tcaaggtttg cctttaccct
      961 ggatttttcc ttgacccata caacgtacgc tcctccggta aagaagacac cgcccgcaat
     1021 tgatccgatc agtgcagcct tgaaaaaata aggaaacagt tttacgaaca gtccgaaaaa
     1081 acttttcaga atggaaaata tagctttcaa ttgattatcc gattcagtta ttttacagga
     1141 ggaagatttc ttgacagagt attgtagatt tcctcagcaa gtccaagaga ctcattctga
     1201 gaaatattct tggcatac
//
LOCUS       DAOQGJ010001600         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94448_length_1218_cov_1.896819, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001600 DAOQGJ010000000
VERSION     DAOQGJ010001600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94448_length_1218_cov_1.896819"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PK683_19975"
     CDS             complement(<1..723)
                     /locus_tag="PK683_19975"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNH10787.1"
                     /translation="MSNLEKQGPMQLTKDQLEIVCTPVDEKLLVLAGPGTGKTHTLVH
                     RIVHLVQEQGLVPYSDLLILSFSRAAIGEIKQRISTMVSSGFSDDLRFLNIRTFDSFS
                     TKLMISLEESVELVGKSYDERIQLATEILSSDNPGAVSILKQFRHVIVDEIQDLVGGR
                     AKLVQQVLRVSNGGYSLFGDPAQGIFDYLVEQSGIGPKSIEFLSSIRGISGNNLKEKV
                     LDENFRAQSPSVSIASNVRSLIT"
     gene            857..>1218
                     /locus_tag="PK683_19980"
     CDS             857..>1218
                     /locus_tag="PK683_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10788.1"
                     /translation="MTGLIENPENNNAENTEDQNIEDFGDLEGLIEPVTDQDIAIEFV
                     DERTLRQLENDGLGRYFRKIGAEVLLSREQEFWYGTDIQTGRKIKQITDDNNFSPSDD
                     LLHHLIDDIESRWKSLSNP"
BASE COUNT          402 a          235 c          228 g          353 t
ORIGIN      
        1 agttatcaga cttctcacat ttgatgcgat agaaactgag ggagattgcg ctctaaaatt
       61 ttcatcaagg actttttctt tcaaattatt gccggatatt cccctaattg atgaaagaaa
      121 ttcaattgat ttaggtccaa tacctgactg ttctacgagg taatcaaaaa taccctgagc
      181 tgggtctcca aacaaagaat atccgccatt acttaccctt aaaacttgtt gaaccaattt
      241 cgcacgacct ccaacaagat cctggatttc gtccacaatt acatggcgaa attgtttgag
      301 aatcgaaact gctccaggat tatcagaaga cagaatttct gttgccaatt gaatccgctc
      361 atcatatgac tttccaacca attcaacaga ttcctccaag gaaatcatta atttggtgga
      421 aaaggagtca aatgttctaa tgtttaagaa acgcaagtca tcggaaaagc ctgaagacac
      481 catcgtagat atccgctgct tgatctctcc aattgcagca cgagaaaaac ttagaataag
      541 caaatcggaa taaggcacca gcccttgctc ctgaaccaga tgaacaatcc tatgcaccaa
      601 tgtatgagtt ttacccgtcc ctggaccggc aagcaccagc agcttttcat caacaggagt
      661 acatactatt tccagttgat cctttgtcag ttgcattgga ccttgttttt ctaaatttga
      721 cataaggact cccatatacc gattatactt atataaattt ttaggtatta tgaataaggc
      781 gaaataataa ttttgcaagg ttttcaaata ccccaccaag gttataatgt tcaaatattg
      841 gttaaggtgg agtttagtga ccggtttaat tgaaaatccc gaaaataata atgctgaaaa
      901 taccgaagat caaaacatcg aggactttgg tgatcttgaa ggtttgatag aacctgtcac
      961 tgatcaggac attgcaattg aatttgttga tgaaagaact ctgcggcaat tagaaaatga
     1021 tggattgggg cgttacttta ggaagattgg tgctgaagtt ctcttaagtc gggaacaaga
     1081 attttggtac gggacagata tacaaacagg cagaaaaatc aaacaaatta cagatgataa
     1141 taacttttcc ccaagtgatg atcttttgca tcatttgatt gatgacattg aaagcagatg
     1201 gaagtctctt tcaaatcc
//
LOCUS       DAOQGJ010001601         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94514_length_1217_cov_3.522375, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001601 DAOQGJ010000000
VERSION     DAOQGJ010001601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94514_length_1217_cov_3.522375"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK683_19985"
     CDS             complement(<1..489)
                     /locus_tag="PK683_19985"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH10789.1"
                     /translation="MRAKILKTLVEFDSEGSHQNFPKSSVKSIRLKPVVLKPPVTKEE
                     VSEYEKEKVRVAESLQNADWSEGEEGKPRLAVLNFTPGAGVSQAEAETVTYLITTKLV
                     QTKLFIIIDRLTMEKIIREETLKCRQGENCEKKIQNRLGANKILTGNVTKLGSRYFIN
                     GNI"
     gene            complement(582..>1217)
                     /locus_tag="PK683_19990"
     CDS             complement(582..>1217)
                     /locus_tag="PK683_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10790.1"
                     /translation="HQYEFCFAEDCDRKYPGLGFFTEAILAVSVTGTVNTFTSGQIIK
                     SADINTNFASLKTAIENIPDWTKSGANAVFSSGGVIVNTSAAANSATVTVNGRISSTT
                     LGVYCGTYNGLGVGGYTGAKALCITACGNSNAHMCTAHEISISRQLGISVTNGPWYSS
                     AAFYNTSSGTLNVTDCLGWTSASSSEVGPITNTPNGQNNLCNAFAPVACCL"
BASE COUNT          305 a          304 c          252 g          356 t
ORIGIN      
        1 aatattgcca tttataaaat atctgctgcc cagcttagtt acatttcctg tcagaatctt
       61 atttgcaccg agtctgttct ggattttctt ttcacagttc tccccctgtc tgcatttcag
      121 agtctcttcg cggatgattt tttccatcgt cagcctgtca atgatgatga agagctttgt
      181 ctgcacaagt tttgtagtga tgaggtaagt gacagtctct gcctccgcct gagaaacacc
      241 tgcacccgga gtaaaattca gaacagcgag tctcggtttc ccctcctctc cctccgacca
      301 gtctgcattc tgaagagact ctgccacacg gaccttttcc ttttcgtatt cagagacttc
      361 ttcctttgtc acaggaggct tcagcacaac cggtttcaga cggatgcttt tcactgaaga
      421 ttttgggaaa ttctgatgac ttccttcaga atcaaattcc accaatgtct tcagtatctt
      481 cgccctcaca ttctgaaaat tttttcctga cctcagctca acagtatccg aaaaaagaag
      541 actcgggaat ataaaaagaa atataaaaat gtatttcatc attacaggca gcaggcaacc
      601 ggagcaaaag cattgcacag gttattctgt ccattaggtg tatttgtgat cggaccgacc
      661 tctgaactgg aagcgcttgt ccatcccaga cagtcagtca cattcagagt tccgctgctg
      721 gtattgtaaa acgcagcact ggaataccag ggtccgttcg tgacactgat tccgagctgt
      781 ctggatatgc tgatttcatg tgcggtgcac atatgagcat tggaatttcc gcatgcagtg
      841 atgcagaggg ccttggctcc cgtatatcct cctactccta aaccgttgta tgtgccgcag
      901 tagactccca gtgtagtaga cgaaatcctc ccgttcactg tgactgtcgc tgaatttgca
      961 gcagcagatg tattcacaat gactcctcct gatgaaaaca cagcattcgc tccgctcttg
     1021 gtccagtccg ggatattttc tatcgcagtc ttcagcgaag caaaattcgt attgatgtcc
     1081 gcagacttga tgatctgccc tgaagtaaat gtgtttacag tgcccgtcac agatacagca
     1141 agaattgcct cagtaaagaa tcccagtccg ggatattttc tatcgcagtc ttcagcgaag
     1201 caaaattcgt attgatg
//
LOCUS       DAOQGJ010001602         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94647_length_1216_cov_3.237726, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001602 DAOQGJ010000000
VERSION     DAOQGJ010001602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94647_length_1216_cov_3.237726"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PK683_19995"
     CDS             <1..1069
                     /locus_tag="PK683_19995"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNH10791.1"
                     /translation="QRFKDSKIQRFKDSKIQRFKEIFCNYSLYLISSLFLFLSPFSNL
                     NADSVKVVILPVEDKSRILGEAKKEEIYDSLSKNLNRTSEFKVIDRGQTKVLLKEMKI
                     EQQGLTKTNEIKLLESSDAILVPTVNTESIKGKILLTLKLNLISRDSTSSQGSGVLEE
                     ASFSEGIDLSVKDLLHTMYPNRYEKPNDSLLTSSKSAALFRSAVLPGWGQYYKNQKWK
                     SASFFIGALGAGGLLYNSYNSYHSNENKFHSLNTQNALLTASAQHTQLSALWFYTQSQ
                     SVHSDIRSSGNQITPLSALLFSLYIYNLADAYFSAQTKPAVWNELNEKRNGFQVGFSR
                     EQSPYSQQTGQSFQVSYQFPF"
BASE COUNT          375 a          242 c          233 g          366 t
ORIGIN      
        1 tcaaagattc aaagattcaa agattcaaag attcaaagat tcaaagattc aaagattcaa
       61 agaaattttc tgtaattatt ccctctactt aatttccagt cttttcctat ttttatcccc
      121 tttttccaat ttaaatgcag acagtgtaaa agtagttatc cttcctgtag aggataaatc
      181 tagaattcta ggcgaagcca aaaaagaaga aatctatgat tctctttcta aaaacctgaa
      241 cagaaccagt gaattcaagg tcattgacag gggacagacc aaggtacttc tgaaggagat
      301 gaagattgaa cagcagggtc tgacaaaaac gaatgaaatt aaactgctgg aaagttcgga
      361 tgcaattctc gtgcctacgg taaatacaga gagcatcaaa gggaagatcc ttctgacttt
      421 gaaactaaat ctgatttcca gagacagcac aagcagtcag ggttccgggg ttctggaaga
      481 ggcttctttt tctgaaggta tcgatttatc tgtaaaagat cttctccata caatgtatcc
      541 caatcggtat gaaaaaccaa atgacagtct gctgacttct tcaaaatctg ctgccctttt
      601 tcgatcggca gttctccctg gatggggtca gtactacaaa aatcagaaat ggaaatctgc
      661 atctttcttc attggagctt tgggtgcagg tggtcttctg tacaattcct ataattcgta
      721 tcattcgaat gagaataagt tccattcttt gaacactcaa aatgcactat tgactgcttc
      781 tgcacagcat acgcaattaa gtgctctttg gttttataca cagtctcagt ctgtccattc
      841 tgatattcgt tcttcgggaa atcagatcac ccctttgtct gctcttctgt tcagtctgta
      901 catttacaat ctggcagatg cgtatttttc tgcacagaca aagcctgcag tatggaatga
      961 gttgaatgaa aaacgaaacg gatttcaggt cggcttttcc agagagcaga gtccgtattc
     1021 acagcaaaca ggacagtcgt ttcaagtgtc ttatcaattt ccgttttaag gagtgatcat
     1081 gaaaaagcat ctatttatac tgattattat ctcattgaat tactgtaaag taaacacaga
     1141 aaccggctct gaacctgagc tgcttttgat ttcagcgttg actaagctcg cggaagtcaa
     1201 accgacagtg actact
//
LOCUS       DAOQGJ010001603         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94652_length_1216_cov_3.173127, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001603 DAOQGJ010000000
VERSION     DAOQGJ010001603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94652_length_1216_cov_3.173127"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..403
                     /locus_tag="PK683_20000"
     CDS             185..403
                     /locus_tag="PK683_20000"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNH10792.1"
                     /translation="MELIEERIKALTPELQREVLDFIDFLVNKYSLFSNKAQYRAKSV
                     GGSLEKYKNSKLQSKEKDAWAVAVKDKK"
     gene            989..>1216
                     /locus_tag="PK683_20005"
     CDS             989..>1216
                     /locus_tag="PK683_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10793.1"
                     /translation="MQIEGSLGLYYGLRFGFNFYEALDFITGIFGADLLNDDTLAPMT
                     EKDRREMLSEIHQQIRGVPLPTQDIGEICSAE"
BASE COUNT          399 a          198 c          287 g          332 t
ORIGIN      
        1 aagcaagcag gggataggtt agcttcatcg gtatcggtga tccggacaaa ctggatgtac
       61 tgatcggaag atttcgagac ttcgcagagt caatccgtgc ctgactgaaa agaatttgac
      121 atattcaggg aaactgtcag gataaccttg gagctgtcag agatgaatgc taaggagaaa
      181 aaatatggaa ctgattgaag aaagaattaa agcgttaact ccggaactgc aaagggaagt
      241 actcgacttt attgattttt tggtcaataa atactcgctg ttttcaaata aggcgcagta
      301 cagggcaaaa tctgtagggg gttccttaga aaagtataaa aacagtaaat tgcaatctaa
      361 agaaaaagat gcctgggccg ttgcagtcaa ggataagaaa taaaagttaa agattgtgtt
      421 taatgattca attccgaaaa cccgccctga acggagcaaa atgaaaatga aacatatcat
      481 cattatattt ctattattct tatcccactg ccagtctgtc tccaattatc tccaataccg
      541 agcctacgat ttccgagata tgtttacaat cggtgtggaa aagaatgtcg ccggaggatc
      601 attctttgtc tggtgttttg gaggaggact acagtatgga agtgaaggaa atggctatgg
      661 gatcagaagc gggagcttag gtgaatacaa aacgggatat agtggagaga ttccgccaga
      721 aagaactttg tcaaaaggca aatacgatag aaagggagat cctgttcgag tagtgaggcg
      781 aataatagta ccttacggga atagtgtttt tattgataac agtattgaaa gtattccgat
      841 ttatacagaa ttgaagcgag atcaaaaaaa gtcttactct gctctgacgt taggaatttt
      901 tccaaatata atttcagtgt attcctactg cgaatatgac cccgaatact gtcgactttc
      961 caagttgggc agaaaatgcg agatgccttt gcagattgaa ggatcattag gattgtacta
     1021 tggacttcgg tttggtttca acttctatga ggcactggat tttattacag ggatattcgg
     1081 agcagatcta ttgaatgatg atacacttgc tccaatgact gaaaaagaca gaagagaaat
     1141 gctgtctgaa attcatcagc agatcagagg agttcctctt cctactcagg atatcggtga
     1201 aatttgttct gcggaa
//
LOCUS       DAOQGJ010001604         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94757_length_1216_cov_1.785530, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001604 DAOQGJ010000000
VERSION     DAOQGJ010001604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94757_length_1216_cov_1.785530"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK683_20010"
     CDS             <1..252
                     /locus_tag="PK683_20010"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH10794.1"
                     /translation="DARYKSSEFDFTSGDRLYIFTDGIFEEFNSKREEFGEERLHSLL
                     VKNQELPINQVFQKILNELDAFLEGKGRQDDLTILGIEY"
     gene            276..>1216
                     /locus_tag="PK683_20015"
     CDS             276..>1216
                     /locus_tag="PK683_20015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH10795.1"
                     /translation="MKKEIILCVDDDVSVLNALRTLISNTISKDYIIEIAESGEEALE
                     ICQDVEAEGNELCAVISDFIMPGMYGDELLVRIHKMSPRSVKIMLTGQSSLQGVKRAI
                     NEANLYRFIEKPFDNSDMMLTLKSAIHAFRQDRELELRNRELEIANTHLEQLVAERTS
                     ELVEKNKQLEKLSITDRLTGLNNRLKLDRVIDEEFARSRLESGIFALILLDIDKFKSI
                     NDTYGHSVGDMVLIEFANLLSAGIRSEDVAGRWGGEEFLIICRNTDLNPAAALAESLR
                     QRVAEHSFPVIKNRTSSFGVSAYQNGDTIAAMLNRAD"
BASE COUNT          365 a          215 c          305 g          331 t
ORIGIN      
        1 gatgcaagat acaaatcatc cgaatttgat tttacttccg gtgaccggct ttacattttt
       61 actgacggca tctttgaaga atttaattca aagagagagg agtttggaga ggaaagactc
      121 cattctttat tggtgaagaa tcaggaactg ccgatcaatc aggtttttca gaaaatttta
      181 aatgagcttg atgctttcct tgaaggaaag ggcaggcagg atgatcttac tatccttggg
      241 attgaatatt agacttggac agtttgcagg aaaatatgaa aaaggaaatc attttatgcg
      301 ttgacgacga tgtttcggtg ctgaatgcac ttagaacatt gatttcaaat actataagta
      361 aagattatat tattgaaatt gcagaaagcg gagaggaagc gctggagatc tgccaggatg
      421 tcgaagcaga ggggaatgaa ctttgtgcag tcatttctga ttttataatg cctggaatgt
      481 acggagatga acttcttgta cggattcata aaatgagtcc aaggtctgtc aaaattatgc
      541 tgacaggaca gagcagtctg cagggagtaa aacgggcaat caatgaagca aatctttatc
      601 gttttattga gaagcctttt gataattccg atatgatgct tactctcaag tctgcaattc
      661 atgcattccg tcaggaccgc gagctggaat tgagaaatcg ggaactcgaa atcgccaata
      721 ctcatttaga gcaattggtc gcagagcgta ccagcgaatt ggtggaaaag aataagcagc
      781 tcgaaaaact ttccattaca gaccggctga ccggtctgaa caaccgactg aaattagacc
      841 gtgtaattga tgaggagttt gcccggagca gactggagag cggtattttt gcgctgatcc
      901 ttctggacat tgataaattc aaatccataa atgatacata cggtcattcc gtgggtgata
      961 tggtgcttat agaatttgcc aacctgcttt ctgcaggcat aagaagtgaa gatgttgcgg
     1021 gacggtgggg cggtgaggaa tttcttatta tatgccgcaa tacagatttg aatccagcgg
     1081 ccgctctggc ggaaagtctg cgtcagagag tggcagagca ttcttttccc gtgatcaaaa
     1141 accggacatc cagtttcggc gtgtccgcct atcagaatgg agatacaatt gcggcaatgc
     1201 tgaacagagc tgacag
//
LOCUS       DAOQGJ010001605         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94819_length_1215_cov_3.120690, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001605 DAOQGJ010000000
VERSION     DAOQGJ010001605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94819_length_1215_cov_3.120690"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..>1215
                     /locus_tag="PK683_20020"
     CDS             161..>1215
                     /locus_tag="PK683_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10796.1"
                     /translation="MLINILKYIIFFTFTFSLRSEKENTVQIEWEKAPGAVYYRLEIK
                     DSQDIAVYSEKTAGTVLNVRLSSGKEYSRRIVSIDKTGEEFASEWTPVLLVSQTEKRS
                     RIRLEWEKNNRSDYYIAEIRDSDGKTLIRRKVKKTETEVELLPGKYEKRILAVDKTGD
                     ILASSWTDLEVIRRETENKKYDIIIRSASFPGWGQVYSFSAYGYDARLRGYSIMTISV
                     LLAGTYAYYVKQAERQKRQFNHYADNAIFFSAAAGDSLFSVQDIYNFSAISHLIEKEK
                     NSVMAGRAANTALGLLGAVYAVQFADAFRIQRYILPKLSMQVGRNFGNSEESFFSFSA
                     GRCQHSIGPGRPGKYCHS"
BASE COUNT          382 a          211 c          279 g          343 t
ORIGIN      
        1 tctgacagtt attctgtgca ggattttact cagctgaaaa gagggtttta cagattttct
       61 gtatcttccg aggacggctc tgtccaagcg gaaactcagt tcagcatcat actcagagaa
      121 attgattcag atgtgaaatt caaaaccccg gaaaaagtat atgttgatta atatattaaa
      181 atatataata tttttcactt ttactttcag tctccgttca gaaaaggaaa atacggtgca
      241 gattgagtgg gaaaaggcac cgggagcggt ttattacaga ttggaaatca aagacagtca
      301 ggacattgca gtttactcag aaaagacggc agggacagtt ctgaatgtga gactttccag
      361 cggaaaagaa tattcaagaa gaattgtatc aattgataaa actggagaag aatttgcatc
      421 tgaatggaca cctgttctgc tggtttccca gacagaaaaa agaagcagaa tccgtctgga
      481 atgggaaaaa aataacagat cagattatta tattgcagaa atacgggatt cagacggaaa
      541 aactctgatt cgcagaaaag ttaaaaaaac agaaacggag gttgaacttc tgccgggaaa
      601 gtatgaaaaa aggattctgg cagttgataa aaccggggac attctcgctt cttcatggac
      661 tgatcttgag gttatccgaa gagaaacaga aaataagaaa tatgatatca ttataaggtc
      721 tgcatctttt cccggctggg ggcaggtgta ttccttttct gcctatggat atgatgctcg
      781 gctcagggga tattccataa tgacaatcag tgtgctgctg gccggaactt atgcttatta
      841 tgtgaaacag gcagaaaggc agaaaaggca gtttaatcat tatgcagaca atgcaatatt
      901 tttcagtgca gctgccggag attctctgtt ttctgtacag gatatttata atttttctgc
      961 tatcagtcat ttgatagaaa aggagaaaaa ctcagttatg gcaggccgag ctgcgaatac
     1021 tgctttaggt ctgctaggtg ccgtgtatgc agttcagttt gcagatgctt ttcgtatcca
     1081 acggtatatt cttcctaaac tttcaatgca ggtcggcaga aactttggaa attcggagga
     1141 gtcctttttt tccttctccg caggaagatg tcaacattct atcggccctg gcagaccagg
     1201 taagtattgc cattc
//
LOCUS       DAOQGJ010001606         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94844_length_1215_cov_2.511207, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001606 DAOQGJ010000000
VERSION     DAOQGJ010001606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94844_length_1215_cov_2.511207"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..>1215
                     /locus_tag="PK683_20025"
     CDS             115..>1215
                     /locus_tag="PK683_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNH10797.1"
                     /translation="MQLLSVSKLSKTIGDRTLFKDVDFGISEGEKLAIVGVNGSGKST
                     LLRILTGKEESDTGEIVKNRELKTSILAQNFHSDPEKTVREHIFRGEGKTILLLKKYS
                     DICERMAEGDANAEKEYNAILTEMDKVSAWEYETQVESILRELGIDHLNLKMSDLSGG
                     MLKKVELASALIEESNLLILDEPTNHLDVQSILWLEDFLVQTQKALILITHDRYFLDR
                     ITDKILEIDSGKCFLYQGNYSYFLEKKAEREEKERIQEEKAEKFLRKEIEWLRRQPKA
                     RTTKQKARIDRADAVINREKYEIQKELELSVAAKRQGKTIMEVKNIRKSFGEKTIIDG
                     FTYHFKKQERLGIVGANGAGKSTLLNILAGRLN"
BASE COUNT          428 a          230 c          295 g          262 t
ORIGIN      
        1 ggaactggaa ggtatccgga aagaaaacaa ggaactggtc gccaagatta cagaaagcgg
       61 agaagtagta gaaagtattt cgacaatagc aaagtatctg aaaaagctgt aaaaatgcag
      121 cttctttctg tttcaaaact ttccaagaca attggagacc gtaccttatt caaggatgtg
      181 gacttcggaa tttccgaagg agaaaaactg gcaattgtcg gagtcaacgg ctccggcaaa
      241 tccacccttc tcaggattct gacaggaaag gaagaatccg atacaggcga aatcgtcaaa
      301 aatcgtgaac tgaaaacatc catccttgct cagaactttc attccgatcc ggaaaaaaca
      361 gtaagagaac atatatttcg cggtgaagga aagacaattc ttcttctgaa aaaatattca
      421 gacatctgtg aaagaatggc tgagggagat gcaaacgcag aaaaggaata caacgccatt
      481 ctgacagaga tggacaaggt ctctgcctgg gaatatgaaa cacaggtaga atccattctg
      541 cgcgaactgg ggatagatca tctgaatctg aagatgagcg atctttccgg gggtatgctg
      601 aagaaggtgg aacttgcatc agcactcatt gaggaaagca atcttctgat tctggatgaa
      661 cctacaaacc atctggatgt tcagtccatt ctatggctgg aagattttct ggttcagacc
      721 cagaaggcac tgatcctgat cactcacgac agatattttc tggacagaat tactgataaa
      781 atattggaaa ttgattcagg aaaatgtttt ttataccagg gaaactattc ctattttctg
      841 gaaaaaaagg ctgagagaga ggagaaggaa agaattcagg aagagaaggc tgaaaaattt
      901 ctccgcaagg aaatcgaatg gctccgaaga cagcctaagg cgagaacaac aaagcagaag
      961 gccaggatcg accgtgcaga tgcggtaatc aacagagaaa aatacgaaat acagaaggaa
     1021 ctggaacttt cagtggcagc caaaagacag ggcaagacca ttatggaagt gaagaacatc
     1081 cgtaaatctt tcggtgaaaa aacgatcatt gacggattca cgtatcattt caagaagcag
     1141 gaaagactgg gaattgtcgg ggccaacggt gcagggaaat caacgcttct caacattctg
     1201 gccggcaggc tcaac
//
LOCUS       DAOQGJ010001607         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94935_length_1214_cov_3.449525, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001607 DAOQGJ010000000
VERSION     DAOQGJ010001607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94935_length_1214_cov_3.449525"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PK683_20030"
     CDS             complement(<1..1187)
                     /locus_tag="PK683_20030"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH10798.1"
                     /translation="MERALEGGIRNPGIQYILELSYDRGEIVKKDYQEKYDLRLEHIQ
                     VDLMNNIILEEIDLIPDERLRENLRKLLLSTHSEFEGYQKSLLSFLDKADTDGQVLRI
                     SLRKYISKLNQTAFYYSNKISLMDEENFCRNSEKLLRKKEDSLFFRQALEKRYRDCLW
                     NGKEISVKELKYNIAKYFRPFKRVEERHFRKSHSDLQHFITYGKYFPATKTIREVGFS
                     YETYRQYMHTPSKLQKIILAFVLIVILFLYPTFFKDSLSSPLMSLLAGVRQVNKGHLN
                     VRLPVKVEDEIGYITGSFNKMVRSIEDSKNKLEDYANRLEEKVEERTKELAYSLAQVE
                     NLKSQQDGDYFLTTLLLNPLSINGSASTKVKVDFFIKQKKEFKFRSRSHDIGGDMCIS
                     QEIT"
BASE COUNT          302 a          281 c          248 g          383 t
ORIGIN      
        1 gtgatttcct gggatatgca catatccccg ccgatatcat ggcttctgga tctgaacttg
       61 aattcctttt tctgcttaat gaaaaagtcc acctttacct tagtgctcgc tgatccgttt
      121 atgctcagag gattgagaag cagtgtggtc aggaaataat ccccgtcctg ctggcttttc
      181 agattttcca cctgtgccag agaatatgca agttcctttg ttctttcctc caccttttct
      241 tccagcctgt tggcgtagtc ctccagcttg tttttagaat cctcaattga cctcaccatc
      301 ttattgaatg atcctgtgat atatcctatc tcatcttcca cctttaccgg aagacgtaca
      361 ttcagatgtc ccttgtttac ctgacggact ccggccagaa gactcatcag cggagaggac
      421 agactgtcct tgaaaaatgt agggtacaga aagaggatga cgataagcac aaatgcaagg
      481 atgatcttct ggagtttgga aggagtatgc atgtactgtc tgtaagtctc ataggaaaaa
      541 ccgacttcac ggattgtctt cgtggcagga aaatattttc cgtaagtaat gaaatgctgc
      601 agatcactgt gactttttcg gaaatgcctt tcctccaccc gtttgaatgg cctgaaatac
      661 ttggctatat tgtatttcag ttccttgacg gagatttcct ttccgttcca gagacagtcc
      721 ctgtagcgtt tttccagagc ctgacggaaa aagaggctgt cttccttctt tcgcagaagt
      781 ttttccgagt ttctgcagaa gttctcctca tccataagcg aaattttgtt tgaataataa
      841 aatgcagtct ggttgagctt ggaaatatat ttgcggagcg atatccttaa aacctgaccg
      901 tcagtatctg ccttgtccag gaaactgagc agagatttct gataaccctc gaattcggaa
      961 tgagtggaaa gcaggagttt tcgcagattc tccctgagcc tttcatcagg aatcagatct
     1021 atttcctcaa gaatgatatt gttcatcaga tccacctgga tatgctcaag gcgaagatca
     1081 tatttttcct gatagtcctt ttttactatc tctcccctgt cgtaggaaag ctccagaata
     1141 tactgtatac cggggtttcg aatccctcct tccagagccc tttccatatt ctctatccgt
     1201 atggaatcaa attc
//
LOCUS       DAOQGJ010001608         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94975_length_1214_cov_2.692839, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001608 DAOQGJ010000000
VERSION     DAOQGJ010001608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94975_length_1214_cov_2.692839"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PK683_20035"
     CDS             <1..863
                     /locus_tag="PK683_20035"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH10799.1"
                     /translation="FGFKPYDSGSSLKTVQGRFVRNDFTGKYEYPAICRYTGKEFSYG
                     ILKKDSETGKTVCDQRRYKADKSYYRNEPFEVLYHLKPLKKNQEIVWEVPNAKENGNE
                     EVYKYSFQPPDEKNVLKFCAVADGFTMSGIDNVFFQTCSQDRQSYMTDTWYHFLLTLV
                     EYDSENTAEDTKPTPELNMQLLKASYKGDADQARIALKSGADPNAQNKGWTGLMYAAY
                     FGKTDVIRALLEYSADTTIKYQGFTALSIAEKKGNTEAAALLKPADRSLRPSILPRSN
                     IAEPSVPGEK"
     gene            863..>1214
                     /locus_tag="PK683_20040"
     CDS             863..>1214
                     /locus_tag="PK683_20040"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNH10800.1"
                     /translation="MKFTWMVFFLTYGAALLAEEIPDFRMRRVDAGQFYTVKVYLQGD
                     RRVYYKTVITRPYQLSATEVTVGQWKAVMGGDAPNYEKEDDKPVTSVSYREIQEFISR
                     LNKKTTGKSYRLPTY"
BASE COUNT          394 a          225 c          284 g          311 t
ORIGIN      
        1 acttcggttt caagccttat gattccggat cctcactgaa gactgttcaa ggaagatttg
       61 tcagaaatga ttttactgga aagtatgaat atcctgcaat atgcagatat actggaaaag
      121 aattttcata cggtattctg aaaaaagatt cagagacagg aaaaacggtc tgcgatcaga
      181 gaagatacaa ggcagacaaa tcctattata gaaatgagcc ttttgaggtt ctctatcacc
      241 ttaagcctct gaagaagaat caggaaattg tatgggaagt gccgaacgca aaagaaaatg
      301 gaaatgagga agtttataaa tattcatttc agccgccgga tgaaaaaaat gttctgaaat
      361 tctgtgcggt tgcagacgga tttacaatgt caggaattga taatgtgttt tttcagacct
      421 gttcacagga cagacagagc tatatgactg atacatggta tcattttctt ctgacactcg
      481 tggaatatga ttccgaaaat actgcagaag acacaaaacc gactcctgaa ctgaatatgc
      541 agctgctgaa agcttcctat aagggagatg cagatcaggc aagaattgcg cttaaatccg
      601 gtgcagatcc gaatgcgcag aacaagggct ggacaggact gatgtatgcc gcatacttcg
      661 gaaaaacgga tgtcattcga gctttgctgg aatacagtgc ggatacaacc ataaaatacc
      721 aggggttcac tgctctgagt attgctgaga aaaaagggaa tactgaggca gcagctctgt
      781 tgaaacctgc cgacaggtcg ttgagacctt ccatacttcc aagatcaaat attgcagagc
      841 cttctgtacc gggagaaaaa taatgaaatt tacatggatg gtattctttt tgacatacgg
      901 tgccgctctt cttgccgaag aaattcctga tttccgaatg aggcgtgtgg atgccggtca
      961 gttttatact gtaaaagttt atctgcaggg ggaccgcaga gtttattata aaacagtcat
     1021 taccagacct tatcagcttt ctgcaacaga agtgaccgta ggacagtgga aagcagtgat
     1081 gggaggggat gctccgaatt acgagaagga ggatgacaag cctgtcacat ctgtttctta
     1141 tcgagaaata caggaattta tttccagact gaacaaaaag acaacgggaa aatcctacag
     1201 acttccgact tatg
//
LOCUS       DAOQGJ010001609         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_94995_length_1214_cov_2.445211, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001609 DAOQGJ010000000
VERSION     DAOQGJ010001609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94995_length_1214_cov_2.445211"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            725..1198
                     /locus_tag="PK683_20045"
     CDS             725..1198
                     /locus_tag="PK683_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNH10801.1"
                     /translation="MKPSIQLISDTLFQETVEKAVSSPRRRSNWNFHELHEVYQRFLN
                     VLVKGTYIQPHMHRDPPKPETFLVLKGELGFLTFDDNGNIINKYLLSEKGPIYGIDIQ
                     PGIWHSIVVLSETCICFEGKSGPYDPSVDKMFAAWAPSEQDESRFKYMENLIKLF"
BASE COUNT          357 a          255 c          237 g          365 t
ORIGIN      
        1 gtgaatgagt aaatttcttt tttccggcga tgatctgaag tccgtaaaaa agaaatttac
       61 tcattcacct tgcgccggcg gtttaattta attataatgt aattagaagt ctttgtatta
      121 tagatcaggt tccatcaaaa ctgaggattc ttttttagag tatttcttga cagtaaatct
      181 gtattttccc ctgtcgtttc cggaattcag caaaaaatag attcggccag ttttatatct
      241 ggacttagta aagaaagggc gggtatgata gatcccattc ttacagcagg ctccatttac
      301 tggacctgaa aaagaacgca gatctatttc aaccgcttcc ttggttgagt cattgtcaaa
      361 ataaaaaaaa tgagcagaga agccatcggg cagtctggga tctttttctg aaagttatcc
      421 ttcaacccat tcattcattt ttacctgttt tgcattatga ggctgaaaaa tattctcttc
      481 tgctctgctg attctttcaa aggacccgaa tgtcaaaaaa cttcccatac cgctgtgacc
      541 gcctcccgac cctctgaaat aggtaaaaga aaattccccg tcacattcaa aagattttcc
      601 ttttaaaacg atttttgcga tgccggagcc gctgtcataa ccgctggagc ggctcaggta
      661 aactgtttct gaaaaatact attgcaaaat attaacaagc atccgagtat agcagttgca
      721 tactatgaaa ccttctatcc agcttatttc cgacacactt tttcaggaaa ctgtagagaa
      781 agccgtgagc agcccccgca gacgttccaa ctggaatttt catgaacttc acgaagtcta
      841 tcagcgtttt ctgaacgtgc tggtaaaagg aacttatatc cagcctcata tgcacagaga
      901 tcctccgaag ccggaaacat tccttgtgct gaaaggagaa cttggatttt tgacctttga
      961 tgataatgga aatataatta acaagtatct tctttcagag aaaggtccga tttacggaat
     1021 tgacattcag ccggggatct ggcacagcat cgttgtttta tcggagactt gcatctgctt
     1081 tgaaggcaaa tcggggcctt atgatccttc cgtggacaag atgtttgcag cctgggcacc
     1141 ctcagaacag gacgaatcca gattcaagta tatggaaaac ctcataaaac ttttttagac
     1201 agatctgcta tatt
//
LOCUS       DAOQGJ010001610         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95036_length_1214_cov_1.783434, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001610 DAOQGJ010000000
VERSION     DAOQGJ010001610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95036_length_1214_cov_1.783434"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..621)
                     /locus_tag="PK683_20050"
                     /pseudo
     CDS             complement(184..621)
                     /locus_tag="PK683_20050"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
BASE COUNT          389 a          263 c          222 g          340 t
ORIGIN      
        1 tgggtttagt agtattccaa aaaaagggta caaaagtcct gccgcaattg gaactcctaa
       61 aatattatac gcaaatgcaa acaacagatt ttgtcgaata tttttcattg tctgctgact
      121 cagacgatgc gcttgtacaa gacccgagag atctcctttc agaagagtca catccttcgg
      181 cagtccaaga agcttaggca gttgacccaa cgcaatgatg agaccgacgc cggaaagata
      241 tccgctgaca accggaaaag gaatatattt tataagacgt ccccccttca gcactccgta
      301 cagaatctgc aaaacagagg aaaaaagggc agtcagagca agcagaccaa tgattttatt
      361 tgcatccaga ccctcttctg tcagctccag tgcaaggccg gatagaactg ctgcagcagg
      421 agcacaggga gcggaaatca gggcagccgt tcttccgaaa agaggagcaa tcactcccat
      481 tgcagcagcc ccaatcatcc cgacaaatgc cccctctccc actttttgag gagaaacagc
      541 agagtagacc agaacaccga atgcgattga agaaggaaga gccacgagca ttgctgcaag
      601 ccctccccag atctcattca tcaatgaatg attttcctta gcgttttcaa gatcgtcaga
      661 cgacataaac tcttccttcc tttgcagtgc aggactgatt tcagagtcta aattctgtta
      721 cagaattgta aactgtcaat ccatttattt ttggtctatg taatccgact catatacttt
      781 ctcaatgtca ccccttttat acagcacaat tcttgtgtgg ctgacttttg gagtccaaaa
      841 agatgtctga tatttttttt tcaaatcatt gtattctttc tttctgttta tatcctccag
      901 tacctgtaat actatctgtc ttgtacaagg ggcattcata taattatgaa aagtatgagg
      961 tctgaacctt tttttattcc cctttaaaag tctctgataa actctatatt tccgtttaaa
     1021 agattcatga agtctgaatg aataccctaa gatgattatt attaatgcgg tcagaatcag
     1081 acgaaacagt gtatctttta aataaaaaaa caaagtgata aatgaaatac agattgaatc
     1141 taagagcaac gcatacaaat tcaatctgat gaaaacctta atttttttca taataaagaa
     1201 aatggagaca ttat
//
LOCUS       DAOQGJ010001611         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95083_length_1213_cov_3.381693, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001611 DAOQGJ010000000
VERSION     DAOQGJ010001611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95083_length_1213_cov_3.381693"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK683_20055"
     CDS             <1..629
                     /locus_tag="PK683_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10802.1"
                     /translation="DFQDIFTAGVERPAYGAGIRISLLPMGFFFQGERDEEEGENKGI
                     GTGVRGGSVGNYYSTQLLFGFLGGETFYYGEVQKDEAGKIQTEEKVPVVAGLRDNLKS
                     HNIKYWDVMNTTPKQRQKENSDKLRKYLAEQIISSTGDESLRQYIPPEKRKPYGYPKS
                     YAWQLEMFLGLRYSVRLGFNIAEFADFLLGFTSYDLLGDDVSGKTLTE"
     gene            763..>1213
                     /locus_tag="PK683_20060"
     CDS             763..>1213
                     /locus_tag="PK683_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH10803.1"
                     /translation="MFYGESEEIRAVDNVSFDISEGELVGYIGQNGAGKSTTIKMLTG
                     ILKPTSGEIRIGELNPYSERKKNAFQIGVVFGQRSQLWMDLPVEESFDLLKSIYEIEE
                     KEYKKKIEFFSDLLGLKDFFRQQARKLSLGQRMKADLAASLLHSPKVL"
BASE COUNT          374 a          224 c          311 g          304 t
ORIGIN      
        1 atgattttca ggacatattc actgcaggag tggaaagacc tgcatacggt gcgggaatca
       61 ggatcagtct tcttcctatg ggattttttt ttcagggaga aagagacgaa gaagagggag
      121 aaaataaggg aatagggact ggtgttcgag gaggcagtgt cggcaattat tattcgactc
      181 agctcctttt cggatttctg ggaggagaga ctttttatta cggcgaggtg cagaaggatg
      241 aagcaggcaa aattcagaca gaggaaaaag tgccagtcgt tgcaggactt cgggacaatc
      301 tgaaaagtca caatataaaa tactgggatg tgatgaacac cactcccaag cagagacaga
      361 aggaaaattc tgacaaactg aggaagtatc ttgcagagca gatcatcagt tcaaccggag
      421 atgaaagtct cagacagtat attcctcctg aaaaaagaaa accgtacggg tatcccaaat
      481 cctatgcctg gcagctggag atgtttttag gacttcgtta ttcagtccgg ctcggcttca
      541 atattgcaga atttgcagac ttcctcttag gattcacttc ctatgatctt cttggagacg
      601 atgtctccgg taaaaccttg actgagtgaa caatgaaaca gcgaagcagc ggagcggcgg
      661 agcgtcagtc tttataagaa cggtatgata aaagcatcta atttatgcaa atttttcaaa
      721 ataagaaaaa gaaagcccgg ttttttcggg gcagttgggt cattgtttta cggagagtct
      781 gaagagattc gtgcggtgga caatgtcagt tttgacatat cggagggaga gcttgtcgga
      841 tatatcggac agaacggagc cggcaagtcg actaccatca aaatgctcac tggaattctg
      901 aagccgacct ccggtgaaat cagaatcgga gagctgaatc cgtattccga aaggaaaaaa
      961 aatgcgtttc agataggggt tgttttcgga cagagatccc agctctggat ggatcttcct
     1021 gtggaagagt cttttgacct tcttaaatcc atatatgaaa tagaagaaaa agagtacaaa
     1081 aaaaagatag aattcttttc cgatctgctt ggactcaagg actttttcag acagcaggca
     1141 aggaaacttt ctctggggca gaggatgaag gcagatcttg cagcaagcct tctccattca
     1201 cccaaggttt tat
//
LOCUS       DAOQGJ010001612         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95099_length_1213_cov_2.977547, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001612 DAOQGJ010000000
VERSION     DAOQGJ010001612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95099_length_1213_cov_2.977547"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="PK683_20065"
     CDS             <1..179
                     /locus_tag="PK683_20065"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009947.1"
                     /note="catalyzes the formation of oxaloacetate from
                     L-aspartate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNH10804.1"
                     /translation="YNRTILTNPLLELRNIVQVAELIILSALQRKESRGLHYTTDYPE
                     NRNPSRSDTVMKNQ"
     gene            complement(181..588)
                     /locus_tag="PK683_20070"
     CDS             complement(181..588)
                     /locus_tag="PK683_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10805.1"
                     /translation="MKTEYYWKKENVLVLRQRAELDRVTAAVEIRFQKEKIHSVEFYS
                     SMLGVAKAYLASQKKTGNAWESEITAQSQSLPNYHYTGFILNGTVTEEFLNRIIADVH
                     LKLKDEVLEKFLKEESDIDEVSDYIMKKYPIAE"
     gene            complement(830..>1213)
                     /locus_tag="PK683_20075"
     CDS             complement(830..>1213)
                     /locus_tag="PK683_20075"
                     /inference="COORDINATES: protein motif:HMM:NF023757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-J related domain-containing protein"
                     /protein_id="HNH10806.1"
                     /translation="LQDYCRDNLKRHLYVHYLKIQLSEKREIFTDAVAEHDPLREYYI
                     NLDNLHGTSEKDVNDMLDSFWKKISAFNRKKDALDILGLDESAREEEIKSRYRELVKL
                     HHPDKGGSEIDFHRIQEAMSILKLL"
BASE COUNT          312 a          259 c          234 g          408 t
ORIGIN      
        1 attacaacag gacgattctt acaaatcctc ttcttgaact cagaaatatt gtgcaggtcg
       61 ctgaacttat cattctttcc gcgcttcaga gaaaggagag caggggactt cattacacta
      121 ctgactaccc tgaaaacaga aacccttcga gatctgatac tgtgatgaag aatcagtaat
      181 ttattctgca ataggatatt ttttcatgat ataatcggat acctcatcga tatccgattc
      241 ttctttcaga aatttttcaa gaacttcatc cttcagtttc agatgcacgt ctgcaatgat
      301 cctgttcagg aattcctccg taaccgttcc gttcagaatg aatcctgtgt aatgatagtt
      361 cggaagagac tgtgactggg ctgtgatttc agattcccag gcattgcctg tttttttctg
      421 agaggcaaga taggcttttg cgactcccag cattgaggaa taaaactcca ctgaatggat
      481 tttctctttc tgaaaacgga tttccactgc tgcagtcact ctgtcaagtt ccgctctctg
      541 tcgcagaaca agaacgtttt cctttttcca gtagtattca gttttcatcc tcggtcgcct
      601 tactgcctga ctgagataaa aatgcaaaat gatatttcag tcaaggaaaa tattttcttc
      661 cggatgttct gcccgctttg atggggttgt gtaaaagcat aaaagaaatg atgctcacgc
      721 gaagacgcgg agttctcgaa gcattgattt cgctccgctt ttgatccaat tcattttatt
      781 tcttttgaga tctgcataaa aacagagttt ttttacagtt tcatccttat cagaggagtt
      841 tcagaattga cattgcttcc tgaattctgt gaaaatctat ttctgaaccg cccttgtcgg
      901 ggtggtgaag ttttacaagt tccctgtatc tggattttat ttcttcttct cgtgcagatt
      961 catccagtcc cagaatgtcc agtgcgtcct tttttctatt gaatgcggag atttttttcc
     1021 agaaggagtc cagcatatcg ttcacatcct tttcagatgt cccgtgcaga ttatccagat
     1081 ttatataata ttccctcaaa ggatcatgtt ctgcgactgc atcagtaaaa atttccctct
     1141 tttccgagag ctggatcttt aagtagtgaa catacagatg tcttttcaga ttgtctctgc
     1201 agtagtcctg gag
//
LOCUS       DAOQGJ010001613         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95128_length_1213_cov_2.536269, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001613 DAOQGJ010000000
VERSION     DAOQGJ010001613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95128_length_1213_cov_2.536269"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PK683_20080"
     CDS             complement(<1..248)
                     /locus_tag="PK683_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10807.1"
                     /translation="MIFILENDEFSGLPEKTIPDVHLYWKEFAEIPKTESARAVPKTV
                     FNAGSDSEKNSKCRLCAGKLAPIRSFLQQTGDELQGNLT"
     gene            complement(359..>1213)
                     /locus_tag="PK683_20085"
     CDS             complement(359..>1213)
                     /locus_tag="PK683_20085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNH10808.1"
                     /translation="VFKKVSTVHLQEHVEKICRIENIQYDNEGLFWIAKKGDGSVRDT
                     LSFMEQAVTFTNGKLIGLSISKMIGYRGVEIQIQFLTDILDANDAGKVFRTVESIYSE
                     GGDLQKFLWDFIEFSHTAILLKENITDRETLNYPLEDIQKIQAAIAHYDSEKLIFLSE
                     EMFMIYEKLSSMRLRNSFEIKVFFEIRLKRLTGQLNKPTLSGVLDRIKNLSEHLQKDI
                     QKLPDAPAPVPAASAISEKKAEPKTEIFSKEMLASGPKNEDSLEDSIKNSFKGVEVNP
                     NDVPDLKN"
BASE COUNT          300 a          254 c          266 g          393 t
ORIGIN      
        1 gtcaggttgc cctggagctc gtccccggtc tgctgcagga aggagcggat cggcgcaagt
       61 tttcccgcac agagtctgca tttggagttt ttttctgaat ctgaccctgc gttgaatact
      121 gttttgggaa cggctcttgc agattctgtc tttggaatct ccgcgaattc tttccagtac
      181 agatgcacat ccggaattgt tttttccgga aggccggaga attcatcatt ttccagaatg
      241 aatatcatat ccttcaggat ggaattgaat tcgattaatt cagattttct gtactgagac
      301 aacacggatt ttttttagcg gagataaaat ggagtgtcaa gaggattttt gaaaaacttc
      361 aattcttcag atcgggaaca tcgttcggat ttacttcgac acccttgaaa ctgttcttga
      421 tggaatcttc cagactgtct tcgtttttag gacccgatgc aagcatttcc ttggaaaaaa
      481 tttctgtctt cggttctgct tttttttccg agattgcaga ggctgcagga acaggggcag
      541 gtgcatccgg aagtttctga atgtccttct gcagatgttc agagaggttt ttgattctgt
      601 ccaaaactcc ggagagagtg ggcttgttca gctgtccagt cagacgcttc agtctgatct
      661 caaagaatac cttgatttca aatgaattcc tgagcctcat cgaagaaagt ttttcataga
      721 tcataaacat ttcctcggaa agaaaaatca gtttttcgga atcataatga gcaattgctg
      781 cctgaatttt ctgtatgtct tcgagaggat aattgagcgt ctctctgtcc gtgatgtttt
      841 ctttcagaag aatggctgta tgagaaaatt caatgaagtc ccagagaaat ttctgaagat
      901 ctccgccttc cgaataaatt gattccactg tgcggaagac ttttcctgca tcatttgcat
      961 ccagaatatc cgtgaggaac tggatctgaa tttccactcc tctgtatccg atcatcttgg
     1021 aaatcgaaag tccgataagt tttccgtttg tgaatgtgac tgcctgctcc ataaaggaaa
     1081 gtgtgtcccg gacggacccg tctccttttt tggcgatcca gaaaagtccc tcattgtcat
     1141 actgaatatt ttctattctg cagatttttt ccacatgttc ctgcagatgc actgtggaca
     1201 cttttttgaa tac
//
LOCUS       DAOQGJ010001614         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95208_length_1213_cov_1.322107, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001614 DAOQGJ010000000
VERSION     DAOQGJ010001614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95208_length_1213_cov_1.322107"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK683_20090"
     CDS             <1..250
                     /locus_tag="PK683_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10809.1"
                     /translation="CPTPSIRHCFPFFYESGKSQKNGKEGCSLEKKEKSMMKIISVLL
                     TISLVGCITYAKANKTNIEVEYTSVQNSQTDNEKKDQK"
     gene            256..1011
                     /locus_tag="PK683_20095"
     CDS             256..1011
                     /locus_tag="PK683_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10810.1"
                     /translation="MKKFFWMFLLVPFVHCNREILQNYAAMGGKPLHCTGENSVTCIS
                     EKPVFEDQRVKNVTPAAGTTLSYKGKAGTDIFRYSNINHISNWVTVPIAETEDDLRIN
                     RTITLYSYGMLIYKIIMFPFTLGMSLVIPHEFDLNDLILLNMGQFSNIDHIKITIGNA
                     GKNDLKSEIHILEALPSFLKFDSLEYSVSFAGMNIDTEGLTVSYDVKSKDKNQYIPII
                     IKPGKKGFESKKILSIKINVKPDEQDFLKNFRI"
BASE COUNT          431 a          186 c          246 g          350 t
ORIGIN      
        1 gtgcccgact ccttcaattc ggcactgctt tccatttttt tatgaatcgg gaaaaagtca
       61 gaaaaacggg aaggaaggat gctctttaga gaaaaaggag aaaagcatga tgaagattat
      121 ttcagtattg cttacaatca gcttagtcgg ctgcattaca tatgcaaagg ccaacaagac
      181 gaatatagaa gtagagtata catctgttca gaattcgcag acagataatg aaaaaaagga
      241 tcagaaatga aggcaatgaa aaagttcttt tggatgtttc tattggttcc gtttgttcac
      301 tgtaataggg aaattcttca gaattatgca gcgatgggcg gaaagccatt gcattgcact
      361 ggtgaaaatt cagtcacctg cataagtgaa aaacctgttt ttgaggatca gagagtaaaa
      421 aatgtaacac cggcagcggg tacgactctg agttataagg gaaaagccgg aaccgatatc
      481 tttcgttaca gcaatataaa ccacatatcc aactgggtaa ctgtaccaat agcagaaaca
      541 gaggatgatc tgcgtataaa ccggactatc actctgtatt cctatgggat gctgatatat
      601 aaaataatta tgtttccatt cactcttgga atgtcgttgg ttattcccca tgaattcgat
      661 ttaaatgatt taattctgct gaatatggga caattcagca atatagatca tattaaaata
      721 acaatcggaa acgcaggaaa aaatgatctg aagtctgaaa ttcatatcct tgaagcactt
      781 cctagttttt taaaattcga ttcattagag tattcagtca gttttgctgg aatgaatatt
      841 gatacagaag gtttgacggt atcatacgat gtgaaatcga aagataagaa tcagtatatt
      901 ccgatcatta taaaaccggg gaagaaaggt tttgagagta aaaaaatcct ttctatcaag
      961 attaatgtaa aaccggatga gcaggatttt ttgaagaatt ttagaatttg aaaatatagt
     1021 tttttatttc aaattcttga atgtcagagt tgccaagaga aaggaggatc agttttgaaa
     1081 aaaatcagtg cgaatttccc aatcttggtt aatgtcataa aaagtcattt tcagatttaa
     1141 gtgagctttc agcgagggtt tttaaagccc tcaaaaaaaa tcaaaagaaa actgctcttc
     1201 ccagaagaaa agc
//
LOCUS       DAOQGJ010001615         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95236_length_1212_cov_4.465860, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001615 DAOQGJ010000000
VERSION     DAOQGJ010001615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95236_length_1212_cov_4.465860"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1157
                     /locus_tag="PK683_20100"
                     /pseudo
     CDS             1..1157
                     /locus_tag="PK683_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
BASE COUNT          366 a          246 c          295 g          305 t
ORIGIN      
        1 gcttccaagg aagaggctgt ggagattgca agaaaaattc attatcctat attgccattg
       61 ctcggcctcc cagaccgagt tatgttctgg gaggccgagc aatggcaatc ctgaacgatg
      121 aggtttccct tctgaagtac atggaacagg cagaggagat ttccgaggac agaccgattc
      181 ttgtggattc atttctggac ggtgcaattg aagtggatgt ggatgcactc tgcgacggaa
      241 aaagagtttt tattgcaggg attatggagc atattgaaga ggcagggatt cactctggag
      301 attctgcctg cattcttcct cctcaaagca tatctcagaa aatgctcgga gagatccgtg
      361 atgccacaag aaaactggca ctcgaacttt cagtcaaggg tctgatcaat attcagtttg
      421 ccatcaagga tgaggagctt ttcattctgg aagtgaaccc aagggcatct aggacagttc
      481 ctttcgtagc aaaagctata ggtctgccta tagtaaaata tgctacattt attatgcttg
      541 ggcgcactct ggatgagctg aaactgggtg acgagttcag tgtaaaactt gtaaatatca
      601 aggaagcagt tcttccgttc aataaatttc caggagtgga tacaattctc ggacctgaga
      661 tgcgttctac tggagaggtg atggggattg cagaaacacc cggcgaggca ttttacaaat
      721 ctcagattat ggcaggagag gaacctccca aaggcggaac aatttttgtc agcatcaacg
      781 acagaacaaa acctgagctg cttccgttca tcagatctct ttccaatctg ggatataatt
      841 taattgcaac agcgggaact cacaaggtgc tttcagaaaa cggtgttctc tcatcgctga
      901 tccacaagat ttatgaggga gcatatccta acgttgtgga ttatatcaag gaaaagaaaa
      961 tcaacctgat catcaatact cctctttcaa gagtgacaag ggatgatgcc taccatattc
     1021 gtcaggcagc aatcaaattc aaggtgccct gcattacaac tgcagaggcg gcaaaggcgc
     1081 tggtcaacgg acttttggaa atgcagaaca agggtctgag cattcgttca ctgcaggaaa
     1141 ttcacaggaa ggcataaaat ggctgaagaa aataaaactg aagaaaaatc gaaactgaat
     1201 ctttcaccta gg
//
LOCUS       DAOQGJ010001616         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95330_length_1212_cov_2.216076, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001616 DAOQGJ010000000
VERSION     DAOQGJ010001616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95330_length_1212_cov_2.216076"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PK683_20105"
     CDS             complement(<1..591)
                     /locus_tag="PK683_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNH10811.1"
                     /translation="MKLVVGISGASGSVYPARFLKKLLETEGQSSLIVSPASIRIFRE
                     VYRTPINNAAEFLDFIINRWKISPVHRISFKPFDDIGADIASGSNRWDATVIVPCSMK
                     TIAGISAGFTDNLIERCADVTLKERRRLILVPRETPYNRIHLKNMLALDEAGAVILPA
                     SPGFYQMPESFDDLGDFIAGRIFSLLGFDAGLYPKWL"
     gene            complement(588..>1212)
                     /locus_tag="PK683_20110"
     CDS             complement(588..>1212)
                     /locus_tag="PK683_20110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNH10812.1"
                     /translation="TAWGFTLFFCFLFVGASYFINIYTFLLSFPALFIVLFYSYTKRF
                     TWLCHFVLGLGIGIAPSGAWIAVRDSLSMVPVYWTLGLMFHIAGFDILYSTQDIDFDR
                     SKGLYSIPSRFGVEKSLWIARTAHIISLGFLFMGGYSAGLGGIYYIFLIITAALFAAE
                     HILVKPDDLSRVPIAFFHINASISVILFIGILADRWNDLLVHFRNLS"
BASE COUNT          384 a          279 c          256 g          293 t
ORIGIN      
        1 tagccattta ggataaagac cggcatcgaa tccgagaagg ctgaatatcc gtcctgcaat
       61 aaagtctccc agatcatcga agctctcggg catctgataa aatcccggag acgcaggaag
      121 gatcactgca ccggcttcat caagagcaag catattcttc agatgaatcc tgttgtaagg
      181 ggtttctctc ggaacaagga tgagtcttct cctctctttc aaagtgacat cggcacatct
      241 ctctatcaga ttgtccgtaa atcctgcact gatccctgca atcgtcttca tggaacaggg
      301 cacaatcacc gttgcgtccc atctgttcga accggaggct atgtcagcac cgatgtcgtc
      361 gaaaggcttg aaactgattc tgtgcacagg agatattttc catctgttta taatgaaatc
      421 cagaaactct gcagcattgt ttatcggtgt tctgtatacc tctctgaata tcctgatgga
      481 tgcagggctg acaatcagac ttgattgacc ttctgtttcc agaagttttt tcaaaaatct
      541 agcaggatac actgatccgg atgcaccgga aatcccaacc acaagtttca tgaaagattc
      601 ctgaaatgca ctaaaagatc attccatctg tcagccagaa ttccgataaa cagtatgact
      661 gaaatggatg cgttgatgtg aaagaatgct atcggcactc tgctcaggtc gtcaggcttt
      721 acaagaatat gctcggcggc aaatagagca gctgtaatga ttaaaaatat ataatatatt
      781 ccgcccagtc ctgcagagta cccccccatg aaaagaaatc caagagagat gatatgtgca
      841 gttcttgcga tccacagaga tttctccact ccgaatctcg acggaatgga atacagtcct
      901 ttcgatctgt caaaatcaat gtcctgagtc gaatacagta tgtcaaaacc tgcaatatga
      961 aacatcagtc caagcgtcca gtacaccggg accatggaaa gagaatctct tacggcaatc
     1021 catgcaccgc tgggggcaat tcctatcccg agcccaagca cgaaatgaca gagccaggtg
     1081 aatcttttgg tataactgta aaaaagcaca ataaaaagag caggaaaaga aagaagaaag
     1141 gtatatatgt taatgaaata agatgcacct acaaacagaa aacagaaaaa gagagtgaat
     1201 ccccaggcag tc
//
LOCUS       DAOQGJ010001617         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95399_length_1211_cov_3.752595, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001617 DAOQGJ010000000
VERSION     DAOQGJ010001617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95399_length_1211_cov_3.752595"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PK683_20115"
     CDS             complement(<1..971)
                     /locus_tag="PK683_20115"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH10813.1"
                     /translation="MQTHEWVNLSMQIFIVSASYLFVYLATNSKRKKKLSALIGLGLV
                     SLYLLLNFFTPRNNEAEFYSHGAFLTVVLVSSLFVSRLINRRFKTEEQDWQSHFSERE
                     LLNRDLKLAGKIQESLFPNDNIVHGIRFTIFRKTQQQIGGDFYDFVKLREGDVGIFLT
                     DVAGHGVASAMVASMIKVLVATIPYSYKLDPSRLLTYLDKKLSGEFQGSHASALYMYV
                     NFPAKSMIIANAGHPYILYSRQRQDFEELKTEGALLGFHFLDPIAENITMSWQAGDRF
                     VIYTDGLIESINSFESHLENTGLIKILNQNSVIKDLELFKQAVLKDIG"
     gene            1002..>1211
                     /locus_tag="PK683_20120"
     CDS             1002..>1211
                     /locus_tag="PK683_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10814.1"
                     /translation="MNSQKSSLSDSIPMKIKLSIAILYFCASISIISNSVFEEAASRL
                     KNNIRKVRFENGLTLLMLKRTTSPTL"
BASE COUNT          361 a          257 c          224 g          369 t
ORIGIN      
        1 ccaatgtcct tcagcactgc ctgtttaaag agttccaaat ccttgattac tgaattctga
       61 ttcagaattt tgatgagtcc tgtattttcc aaatgagatt caaatgaatt gatagattcg
      121 ataagaccgt cagtgtaaat cacaaatctg tcgccggcct gccagctcat tgtgatattc
      181 tctgcaatcg gatcgagaaa atgaaaacct agaagagctc cttcagtctt gagttcctca
      241 aaatcctgtc tctgtctgct gtaaaggata tatgggtggc cggcgttcgc aataatcatg
      301 gatttagcag gaaaatttac atacatataa agtgcggatg catgacttcc ctgaaactcg
      361 cctgaaagtt ttttatccaa atatgtcagc agtctgcttg gatccagttt gtatgaataa
      421 gggattgtag ctacaagcac tttaatcata gatgctacca ttgctgatgc caccccatga
      481 cctgctacat ctgtcagaaa aatcccgaca tctccttccc tcagttttac gaaatcataa
      541 aaatctccgc caatctgctg ctgagtcttt cgaaatattg taaatcggat tccatgcaca
      601 atattgtcgt ttgggaacag ggattcttga attttgcctg caagtttcaa atctcgattt
      661 aaaagttctc tttcactgaa atgagactgc cagtcctgct cttccgtctt gaatcttctg
      721 ttgatgagcc gactgacgaa aagactggaa acaaggacga ctgtcaaaaa agctccgtga
      781 ctgtagaatt ctgcctcgtt gtttctggga gtaaaaaaat tcagaagaag ataaagactg
      841 acaagaccga gtccaattaa ggcagaaagc tttttctttc ttttggaatt tgtggcaaga
      901 tatacaaaca gataggacgc cgatacaata aaaatctgca ttgaaagatt gacccactcg
      961 tgtgtctgca tctgcttcac agattctgaa attcgacttt tttgaatagc caaaaatcct
     1021 ctctttctga ttctattccc atgaaaataa agctttctat agcaattctg tatttttgtg
     1081 catcaatttc cattatttca aattcagtct ttgaagaagc agcttccaga ctgaaaaaca
     1141 atatccgaaa ggttcggttt gaaaacggtc tcactcttct aatgctgaag agaacaacct
     1201 cgcctactct g
//
LOCUS       DAOQGJ010001618         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95434_length_1211_cov_2.730969, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001618 DAOQGJ010000000
VERSION     DAOQGJ010001618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95434_length_1211_cov_2.730969"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..617
                     /locus_tag="PK683_20125"
     CDS             72..617
                     /locus_tag="PK683_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10815.1"
                     /translation="MIQGIPGAISIVANKTKMSTKLTEDDVEKYLYDIDLSYIKMFSD
                     FNSLSAASNCRAIRQPASKLGGPKSAAMQQHWSADRPFTCRKSIQFNAGIFDFFSKSC
                     NLFQIVFFITNSLISLASSKYRKASSFVGSFSISSYLTLIPISFKLEVDAIINIEKPE
                     SISAHFLNSAEISCFVFIFSL"
     gene            complement(729..1091)
                     /locus_tag="PK683_20130"
     CDS             complement(729..1091)
                     /locus_tag="PK683_20130"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNH10816.1"
                     /translation="MITETADHSVLKNVLKDPIVIKFKETLEKLDVPVHSIYFFGSRA
                     KGTSEEDSDYDFLVLLHDVPDKEKLKEIKGKIIIAIHDEILNTDFDIMIRNLTVFEEN
                     KNVMNTIYNNIYEEGFLI"
BASE COUNT          363 a          251 c          197 g          400 t
ORIGIN      
        1 aggaaaatca tcgaatgctg ggagcaaatc catcaacata gacaaagcag agaatgtctt
       61 cgaatcgttg aatgattcaa ggaattcctg gagcgatatc catagttgca aacaaaacga
      121 agatgagcac gaagctgaca gaagacgatg tggaaaaata tctatacgat attgatttga
      181 gctatataaa aatgttttct gattttaatt ctttatctgc tgcttcaaat tgcagagcca
      241 tcaggcagcc agcgtcaaaa cttggcggcc caaaaagcgc agcgatgcag cagcattggt
      301 ctgctgaccg cccattcacc tgccggaagt ccatccagtt taatgcaggc atatttgact
      361 tcttttctaa aagctgtaac ctttttcaga tagttttttt tatcacgaat tctttaattt
      421 ctctggcaag ctccaaatat cgaaaagctt cttcctttgt tggatcattt tcaatatcat
      481 catatctgac actcattcca atatcattta aattggaagt ggacgcgata ataaatattg
      541 aaaaaccgga atcaatttct gcacactttt taaacagtgc cgaaatatca tgttttgtat
      601 ttattttttc cttatgaaaa ttcaaatatg ctttgagaac tttctcaacg gcctgctgct
      661 gtaagtcagt tcggcagttc ccagatcatg ttctgccttc agtaaataat ttttcgtgac
      721 atcggtcttc aaatcagaaa tccttcttca tatatattat tatatattgt attcattaca
      781 tttttattct cttcaaaaac ggttaaattt cgaatcataa tatcaaaatc cgtattcaag
      841 atttcatcat gaatggcaat gatgattttc cctttgattt cttttaattt ttctttatcc
      901 ggaacatcat ggagcagaac caaaaagtca taatcactgt cttcctcaga cgttcctttc
      961 gcacggcttc cgaaaaaata tatgctgtga accggaacgt ccaatttctc caatgtctct
     1021 ttaaatttga tgacaatcgg atctttcaat acatttttca gaactgaatg atctgctgtt
     1081 tccgtaatca taaattctcc tttttacatt ttaaatcatt ctccccatag ggatgaacgg
     1141 ccgtttgtcc ctatgggttc ttctcccttt ccgccgcaag gctgctgtac agagacaccg
     1201 aaaaaggaac a
//
LOCUS       DAOQGJ010001619         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95489_length_1211_cov_2.087370, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001619 DAOQGJ010000000
VERSION     DAOQGJ010001619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95489_length_1211_cov_2.087370"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..>1211
                     /locus_tag="PK683_20135"
     CDS             31..>1211
                     /locus_tag="PK683_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10817.1"
                     /translation="MFRTFHGAGTADTENKSVGLVSRFEKFSLVNFPGILVKILHTEA
                     SSGQTEGFLKLDYSQENIKMEGDINFLDIDTEDSQDNPVQLSGFYFNTKFSFFRDQED
                     RKAPESYFNRKVSNPNFFLIENVFTSRKNLKRKLYQFESSNLKNLSKIFSLPEGRTIS
                     GKAKLNLIAEETGKNPSWTVLDGNLSLENFSFRSAAPSFSADISKAVITVNPEGEISS
                     KMNGKLFDKEFSLENSGTLNLGYDSAKDRTYFGGQLNLAVNVRKFILKDIFSLIESAY
                     SSVQENIRERQEKMLPEAYITDSKMYQIFLDKLNYSLVFDLENSSMAENSAPWGNFLL
                     TAKMSKGVLDAALQLKTPAGNDAEAKLSLNGQFDKKSPYFDVRVHTKNFPWKEKVYSF
                     CG"
BASE COUNT          374 a          254 c          259 g          324 t
ORIGIN      
        1 catgtcgagt acagcgattc gttctttaaa atgttccgta catttcacgg tgccggaact
       61 gcggacactg agaataaatc agtcggcctt gtttcccggt tcgaaaaatt cagtcttgta
      121 aatttcccgg gcatacttgt caagatcctg catacagaag ccagttcagg tcagacggaa
      181 ggttttctca aacttgatta ttctcaggaa aacatcaaga tggaaggtga tatcaatttt
      241 ctggacattg atactgaaga cagtcaggat aatcctgttc agctgagcgg attttatttc
      301 aatacaaagt tttccttttt cagggatcag gaggacagga aggcccctga atcttatttc
      361 aacaggaaag tcagcaatcc caattttttc ctgattgaga atgtatttac atccagaaaa
      421 aatctgaaac ggaaactcta tcagtttgag agttccaatc tgaaaaacct ttctaaaatt
      481 ttttctcttc ccgaaggaag gacaatttca gggaaagcca agctcaatct gattgcagag
      541 gagactggaa aaaatccttc ctggactgtg ctcgacggaa acctaagtct ggagaatttt
      601 tcattccgct ccgcagcccc gtcgttcagt gcagatattt caaaagcagt aatcacagtg
      661 aacccggaag gcgagatcag ttccaagatg aatggaaaac tttttgacaa ggaattttct
      721 cttgaaaact caggcacact gaatctcggc tacgactctg caaaggacag aacatatttc
      781 ggagggcagc tgaatcttgc agtcaatgtc aggaaattta tactgaaaga catattcagt
      841 ctgatcgaat ctgcctacag ttccgttcag gaaaatatca gggaaaggca ggaaaaaatg
      901 cttccggagg catatatcac tgattccaaa atgtaccaga ttttcctcga taagctgaat
      961 tacagccttg tttttgatct ggaaaattct tcaatggcag agaattcagc accttgggga
     1021 aattttcttc tgaccgcaaa aatgagcaag ggcgtgctgg atgcagccct tcagctgaaa
     1081 actcctgccg gaaacgatgc agaagcgaaa ctctcactga acggtcagtt cgacaaaaaa
     1141 agtccttact tcgatgtaag ggttcataca aaaaactttc cctggaagga aaaggtctat
     1201 tccttctgcg g
//
LOCUS       DAOQGJ010001620         1210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95615_length_1210_cov_2.325541, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001620 DAOQGJ010000000
VERSION     DAOQGJ010001620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95615_length_1210_cov_2.325541"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK683_20140"
     CDS             complement(<1..444)
                     /locus_tag="PK683_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNH10818.1"
                     /translation="MSRLTAKREFLSGRSMGGDEAFFLIAGPCVMEDRDLLDRVAGEM
                     KSVCEELGIFYIFKSSFDKANRSSVNSYRGPGLAEGIKHLEFIKKKYNVPVLTDVHET
                     AHIEPLKDVLDVYQIPAFLCRQTDLIEAAARTGKWVNVKKGQFLAP"
     gene            complement(489..>1210)
                     /locus_tag="PK683_20145"
     CDS             complement(489..>1210)
                     /locus_tag="PK683_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNH10819.1"
                     /translation="FQNKPTASGEPFDRFKLTGAHRTLPFGSEVKIQNLENNKETVVR
                     INDRGPFNKARIIDISEKAAEALDFKDSGIARVGISVVKKTDKKPVKEKDDFSFDEED
                     DDDDDDLDDDDVPSPPKKDTKPAPNTVKPVPEKTVPDKKPSVVKPVPAKETKPKETKT
                     ASEGEQPKGLSVQIGVFKEKMRAENFRSQIKASFTEPVFMFNRNGTYVVQIGDFSSRT
                     DAMDLKEKLKAKGIFGFIPPK"
BASE COUNT          274 a          275 c          238 g          423 t
ORIGIN      
        1 gggtgcaaga aactgtcctt tttttacatt gacccatttg cctgttctgg ctgccgcttc
       61 aatcagatca gtctgtctgc ataaaaaagc aggaatctga tatacatcga gcacatcttt
      121 cagaggttct atatgggctg tttcatgtac atctgtgaga acaggtacat tgtatttttt
      181 tttaatgaat tcgagatgtt ttatgccttc tgcaaggccc ggtcccctgt aagaatttac
      241 agaggatctg ttcgccttgt caaagctgct tttgaatatg tagaaaatgc cgagttcttc
      301 gcagacagac ttcatttcac cggcaaccct gtcgagaaga tctctgtctt ccataacgca
      361 gggacctgcg atcaggaaaa aagcctcatc gcctcccatg gatctgcctg ataaaaactc
      421 tcttttagct gtcagtctgc tcataatgtc agaatatcct ttccttattt tttataaaga
      481 atttatcctt atttaggagg aataaatccg aatattcctt tagccttcag tttttctttc
      541 agatccattg catcagtcct ggaactgaag tctccaatct gaaccacata tgttccgttt
      601 ctgttgaaca taaatacagg ttcagtgaaa gaggccttga tctggcttct gaaattttct
      661 gccctcattt tttccttgaa taccccgatc tgtactgaaa gacctttcgg ctgttctcct
      721 tcagaagcgg ttttagtttc cttcggttta gtttcctttg caggaaccgg ttttacaact
      781 gaaggttttt tatcaggtac agttttttcc ggaacgggct ttacagtgtt cggtgccggc
      841 tttgtatctt ttttcggagg agatggaacg tcgtcgtcat cgagatcgtc atcgtcatcg
      901 tcgtcttctt catcgaaact gaaatcgtct ttttccttga caggcttttt gtccgtcttc
      961 ttgactacag aaattcccac tctggcaatc cccgaatcct taaagtccag tgcctctgct
     1021 gccttttctg aaatatcaat gattctggct ttgttgaatg gccctctgtc attgattctg
     1081 acaacagttt ccttgttgtt ttctaaattc tggattttta cttcagatcc gaaaggaagt
     1141 gtcctgtgtg ctccggtcag cttaaatctg tcaaacggtt ctccgcttgc tgtgggcttg
     1201 ttctgaaact
//
LOCUS       DAOQGJ010001621         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95802_length_1209_cov_1.854419, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001621 DAOQGJ010000000
VERSION     DAOQGJ010001621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95802_length_1209_cov_1.854419"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /gene="flgA"
                     /locus_tag="PK683_20150"
     CDS             complement(<1..861)
                     /gene="flgA"
                     /locus_tag="PK683_20150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNH10820.1"
                     /translation="MRKAFYAICLILFIFPLYSEPAVYLKPRSVLRTTIVKLSDVAKF
                     PPEIQDRELKISVSEPTRILPETLREELPGIKVLGKDCLLIPLNRAVDSEEILSSLSK
                     EINRKTGADFSSFRLQMEDSSVFLPSKSVDFKWSNPGRHLSPGQKIIQLEVFFQGERV
                     YSERIRYTVEMKTEYYAAKHPILKTQKLSEQDFEKKSEFLLEPMTDLWKESVGGLTAL
                     VNLEAGAILRKKHVRNSFAVDKGSEVELFFSEGNITVKTKGIARQSGNRGDRIKLVTA
                     NRTVLTGRVID"
     gene            complement(873..>1209)
                     /locus_tag="PK683_20155"
     CDS             complement(873..>1209)
                     /locus_tag="PK683_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNH10821.1"
                     /translation="SEQGEVTVKIGNEIRPTVIGQIELYRFVNPAGLQAIGRNLFKET
                     VASGPEIPGTPGMEGFGSILQGFLEMSNVKIVEEMVNMIVAQRAYESNSKAIQTSDSM
                     LATAISLKR"
BASE COUNT          325 a          283 c          236 g          365 t
ORIGIN      
        1 gtcaattact ctgcctgtca gaaccgtcct gtttgctgtg acaagtttga tcctgtctcc
       61 cctgtttccg gactgcctgg caatgccttt tgtttttaca gtaatattcc cttcagaaaa
      121 aaaaagttcc acttctgaac ccttgtctac agcaaagctg ttccgcacat gtttctttct
      181 gagtattgca cctgcttcca gattgaccag tgcagtcagg ccgccgactg attctttcca
      241 gagatctgtc atcggctcca gaagaaactc cgattttttt tcaaagtcct gttccgaaag
      301 tttctgtgtt ttgagtatcg gatgttttgc tgcataatac tcagttttca tttcgactgt
      361 ataacgaatt ctttccgaat acaccctttc accctgaaaa aacacttcca gctgaatgat
      421 tttctgaccc ggacttaaat gacgccccgg gttgctccat ttaaaatcga ctgatttgga
      481 aggaagaaaa acggaggaat cttccatctg aagacgaaaa gaggaaaaat ctgccccagt
      541 cttcctgttg atttccttgg aaagagagga aagaatttct tctgaatcta cggctctgtt
      601 caaaggaatc aaaagacagt ctttcccgag aaccttaatt ccaggcagtt cctctctcag
      661 cgtttccgga agaattctgg tcggctcaga gactgaaatt ttcagctccc tgtcctggat
      721 ttcaggaggg aattttgcta cgtcggaaag tttaacaatt gtagttctca gaacagatct
      781 gggtttcaga tagactgcag gttctgagta aagaggaaaa ataaaaagga tgagacagat
      841 tgcataaaat gcctttctca tcctgttaaa aattatctct tcaatgaaat cgctgtagca
      901 agcatactgt cagaggtctg gattgccttg gaattagatt cgtaagcgcg ctgagccaca
      961 atcatattca ccatttcttc cacgattttg acattgctca tttccagaaa tccctgcaga
     1021 atgcttccaa aaccttccat tccaggagtt ccaggaatct caggaccgga agcaacagtt
     1081 tccttgaaaa ggtttcttcc gattgcctga agacctgcag gattcacaaa tctgtacaat
     1141 tcaatctgac cgatcactgt gggacgaatt tcatttccaa tttttacagt cacttcaccc
     1201 tgttcgctg
//
LOCUS       DAOQGJ010001622         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95846_length_1208_cov_3.882047, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001622 DAOQGJ010000000
VERSION     DAOQGJ010001622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95846_length_1208_cov_3.882047"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1208)
                     /locus_tag="PK683_20160"
     CDS             complement(<1..>1208)
                     /locus_tag="PK683_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH10822.1"
                     /translation="EVENAYKKLEEWDSGGRKEDYHKVRLDALLEVGPSVFFSLLIIS
                     AAFIPVFSLTDQEGRLFKPLAYSKNLTMAIAAVLAVTMDPAFRMLFTRMEPFRFRNRF
                     LSEAATALLVGKYYPEEKHPVSRVLFRIYEPACRYVLDHPKKIIISSVILMIFTVPVY
                     LSLPVRFMPPLYEGSLLYMPTTLPGISVAETSRVLQIMDRKIRSFPEVETVYGKAGRA
                     ETSTDSAPFSMMETVIELKDRKHWRKREMECIRDFSVLEGICRKAPFLFRGIAYIIYP
                     NIITKDDLISMMNDELQIPGFINAWTMPIKARIDMLSTGVRTPIGIKILGSDLQEIQR
                     IGTEIERTLREMPETGAAFAERTNTGYFLDIRLDRDELSRYGLTVADANRIISTAVGG
                     ETISVTVEGR"
BASE COUNT          319 a          289 c          262 g          338 t
ORIGIN      
        1 ctctcccttc cactgtcaca gaaatagtct ctcctccgac tgcagtgctg atgatcctgt
       61 tcgcatctgc gactgtgaga ccgtaccggg aaagctcgtc tctgtcgagt ctgatgtcga
      121 ggaaataacc tgtatttgtt ctttctgcaa atgcggctcc tgtttcaggc atctcccgga
      181 gagttctctc tatttctgtt cctattctct ggatttcctg aagatccgat ccgaggatct
      241 tgattcctat cggagtcctg acacccgtgg aaagcatgtc aatcctcgcc ttgatcggca
      301 tcgtccaggc attgatgaat ccgggaatct gaagctcatc attcatcatc gaaatcagat
      361 catccttggt aatgatattg ggatatatta tatatgcaat tcccctgaaa agaaaagggg
      421 cttttctgca gatgccttca agaacggaaa aatctcttat gcattccatt tctcttttcc
      481 tccagtgttt tctgtccttc agttcgatta cagtttccat catggaaaac ggcgcggaat
      541 cagtggaagt ctctgcccgt cctgcctttc cgtacactgt ttccacctca ggaaaacttc
      601 tgatttttct gtccatgatt tgaagcaccc ttgatgtctc tgccactgag atccctggaa
      661 gggtcgtagg catataaaga agtgaaccct catacagagg aggcataaaa cggacaggaa
      721 gagaaagata gaccggaaca gtgaatatca ttaatattac agaagatata atgatcttct
      781 tcggatgatc cagaacatat ctgcaggcag gctcatagat tcggaaaaga acccggctca
      841 caggatgttt ttcttcagga tagtatttcc ctacaagaag tgcagtggct gcttccgaga
      901 gaaatctgtt cctgaaacgg aaaggttcca ttctggtaaa cagcatccgg aatgcagggt
      961 ccattgtcac agcaagcaca gcagcaatcg ccatagtcag attcttggaa taggcaagag
     1021 gcttgaaaag tcttccttcc tgatctgtga gggaaaaaac cggaatgaat gcagctgaaa
     1081 tgataaggag ggaaaaaaat acggaaggac cgacttccag gagagcatcg agcctgacct
     1141 tatgatagtc ttcctttctc cctcctgaat cccattcttc cagttttttg tatgcattct
     1201 ccacttcc
//
LOCUS       DAOQGJ010001623         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95847_length_1208_cov_3.771899, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001623 DAOQGJ010000000
VERSION     DAOQGJ010001623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95847_length_1208_cov_3.771899"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PK683_20165"
     CDS             <1..809
                     /locus_tag="PK683_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10823.1"
                     /translation="KAAVLGSLIISVCILYFILFSDLRIDFTDAHVTNPRFYPMKDFF
                     SLNHFYEIISCIFMTSLPSVSVTVYFIFFRREEWKSFYTSSQGRFLLFLLLGFLIHAS
                     VHNPQLGFPRDWDLMSFYWIPFSLIAYRLLLHSVIQRSELFPVFIFSLCILLSQGYYL
                     NIQKNEDTEKLTFLRNSVSEFSAGFQSVKASVPPKERKFFTRASFFLFRTEKKLLTSE
                     GREREKLLEKNSELKSLLSEYSGSWDRNLKIRFIRSATEFHHQYLQFVRP"
     gene            945..>1208
                     /locus_tag="PK683_20170"
     CDS             945..>1208
                     /locus_tag="PK683_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH10824.1"
                     /translation="MPDLLKISNLYANIGDKEILKGVNLTIRTGEIHAIMGKNGSGKS
                     TLSNIIMGHPNYTVTQGDIEYKGQSILGLRTDERARLGLFLSFQ"
BASE COUNT          322 a          249 c          245 g          392 t
ORIGIN      
        1 tgaaggccgc tgttctcggt tcactcatta tatcagtgtg catactgtat ttcattttat
       61 tttctgatct gagaatcgac tttacggatg ctcacgtgac aaatcccaga ttttatccga
      121 tgaaggactt cttctctctg aatcattttt atgaaatcat ttcatgcata ttcatgacat
      181 cgcttccttc cgtgtcagtg actgtatatt ttatattctt cagaagagag gaatggaaga
      241 gtttttacac atcctcgcag ggaaggtttc ttctgtttct tcttctgggt tttctgattc
      301 atgcttccgt gcacaatcct cagcttggtt ttccccgcga ctgggacctg atgagtttct
      361 actggatacc tttttctctg atagcgtaca ggcttctcct tcattcagtg attcagcggt
      421 ctgaactgtt tcctgtattt attttttcat tatgtattct tctttcacag ggttattatc
      481 tgaacattca gaaaaatgag gacactgaaa agctgacctt tctgagaaat tcagtctccg
      541 agttttccgc cggttttcag tccgtcaaag cttccgttcc tccgaaggaa agaaagtttt
      601 ttacaagggc ttcctttttt ctcttcagaa ctgaaaaaaa acttttaacc tcggaaggca
      661 gagagagaga aaaacttttg gaaaaaaact ctgaattgaa atcgcttctt tcagaataca
      721 gcggttcatg ggacagaaat ttgaagatca gattcatccg gtcggctacc gaatttcatc
      781 atcagtatct gcagtttgtc cgtccttaaa tgttccggga cctgaaggct ggatgctgtc
      841 ttaacccgta gggtctgcat tccagcggct cagttttatt tttagaatga ttataaaaat
      901 aactttactt gcaatatcca gtttaaaaaa aagtttggta ctatatgcca gatctcttaa
      961 aaatcagtaa tctttatgcg aatatcgggg ataaggaaat cctgaaaggc gtgaatctga
     1021 ccatacggac aggggagatt catgcaatta tgggcaagaa cggttccggt aaaagcactc
     1081 tttccaacat cattatgggg catccgaact acacggtgac acagggtgat atagaataca
     1141 agggacagtc cattctggga ctgagaaccg atgaaagagc ccgactgggt cttttccttt
     1201 cattccag
//
LOCUS       DAOQGJ010001624         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95856_length_1208_cov_3.383348, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001624 DAOQGJ010000000
VERSION     DAOQGJ010001624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95856_length_1208_cov_3.383348"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PK683_20175"
     CDS             <1..402
                     /locus_tag="PK683_20175"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10825.1"
                     /translation="DEAGYLISSAVGSKLLPTGKRALIIDAGVNLLYTSTWYDFKISP
                     VKNDSHHCEDTILYGPLCMNIDIVRESCLLPNMSKGEKLVIWPVGAYNVTQWMQFIEM
                     RPAVVMIKSDSSVKMIRRKETVDDINAMEME"
     gene            403..>1208
                     /locus_tag="PK683_20180"
     CDS             403..>1208
                     /locus_tag="PK683_20180"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNH10826.1"
                     /translation="MELSGIYRNFILSAFRSYSTLIFSTSPSKGFLLFLVTMFSPKAG
                     LCGLLGIFFSNLIAFFLKVDRERISKGLYGFNGLLVGLSFSMHYELDTSLVLILLAAS
                     MMLVCVTLTLEHMLSYFLGLPVLSIPFVMISISSSYAFFNYHSLTHSNAFVLELDKYF
                     PEINRYILFYLKSLGGIFFQTSPWAGLAIFFILAAASRISLMLSIIGFSLGTGFHILL
                     LGDSSDLQSGIIGFNCVLTAIAVGGVFLVPSPHTFVYAGLAAVCSALFAS"
BASE COUNT          291 a          286 c          252 g          379 t
ORIGIN      
        1 gatgaggcgg gctatctcat cagttccgca gtgggttcaa aactcctccc tacaggcaaa
       61 cgcgctctta tcattgatgc aggagtgaat ctgctttata cttccacctg gtatgacttt
      121 aaaatctctc cggtaaaaaa tgactctcac cactgcgaag atacgattct ttacggtcct
      181 ctctgcatga acatcgacat agtcagagaa agctgccttc ttccgaatat gtccaaagga
      241 gaaaaacttg tcatctggcc tgtcggtgcg tacaacgtca cacagtggat gcagttcatt
      301 gaaatgcgcc cggccgtcgt aatgatcaag tcggattcct ctgtgaaaat gatcagaagg
      361 aaagaaactg tagatgatat aaatgctatg gaaatggagt aaatggaact gtcaggaatc
      421 tatagaaatt ttatcctttc cgcattcaga tcctactcaa cactgatttt cagcacaagc
      481 ccttccaagg gctttctgct ttttttggtc accatgtttt cccccaaggc aggtctatgc
      541 ggtctccttg gaattttctt ttcgaatctg attgccttct ttctgaaagt ggacagagaa
      601 agaatcagca agggtctgta cggtttcaac ggtctgcttg taggactctc cttcagtatg
      661 cactatgaac tggacacaag ccttgtactg atacttcttg ctgcatcaat gatgcttgtc
      721 tgcgtcactc tcactctgga acatatgctt tcctattttc tgggtctgcc ggttctttct
      781 attccctttg taatgataag catcagctcc tcttatgctt ttttcaatta ccacagtctg
      841 actcactcga atgctttcgt gcttgaactg gacaagtatt ttccggaaat aaacaggtac
      901 attctttttt atctgaaaag tctcggcgga atattttttc agacaagtcc ctgggcggga
      961 ctggcaatat ttttcatact ggccgccgcg agcagaatat ctctgatgct ttccatcatc
     1021 ggtttttctc tcggaacagg gtttcatatt cttctgctcg gagacagttc tgatcttcag
     1081 tcagggataa tcggattcaa ctgtgttctc acagccattg cagtaggagg agtttttctc
     1141 gttccctccc ctcatacatt tgtatatgcg ggacttgcag cagtatgcag cgcgctgttc
     1201 gcatcctt
//
LOCUS       DAOQGJ010001625         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_95935_length_1208_cov_2.121422, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001625 DAOQGJ010000000
VERSION     DAOQGJ010001625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95935_length_1208_cov_2.121422"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(777..>1208)
                     /locus_tag="PK683_20185"
     CDS             complement(777..>1208)
                     /locus_tag="PK683_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10827.1"
                     /translation="IFVFIGVLVVFFIFWVVLMKLLHTVSGWFALQESFGTETVPSGR
                     EESFSYVQIGWLSYNGVIHICFTEKGLYMDIFVLFRFGAGPVLIPWDNLIFKEKKRIL
                     WMDYVNLEAQLRNGKKIRLQFLDRIFTEYDLKKIRGYYATN"
BASE COUNT          339 a          230 c          239 g          400 t
ORIGIN      
        1 atgcttatta gcttagttgc tgtttgctgt ttgctgtttg ctgtttgctg tttgcacaga
       61 ttttattttc attgtgattt ctccaatcag tttatttcaa aaaagtgcct atttgccttt
      121 ttgtactgtt tagcattgaa gtctgtctga tgtccagttt tttatgaggc ttgctgacgg
      181 atttagtcat tttctctgca aaatagcggt ggaatgggtg ctggagaaga tcttatgagt
      241 tttttacttt gttgatgttt gtaacaggat aagtgtttca gtttatatct gatatctgtt
      301 attaccagat atgccgaagt ctatatggta catattcctt gaacttttta tcctttgatt
      361 tcctgtcaaa tcggaagtct gtcaaaggga tcaaaatgct cgactttgga aagtaaataa
      421 aaggaacttg ccgcactttc ctcaaaggat atatttcaaa tccagaatca cggcagctct
      481 tctgaagcca catttgacag tttaagcttg tctatgctga gtttaagtga tatcttccaa
      541 gaagtcaatg tgcttatgca tatctcattc tcttcgtcaa gcactgctga tttcgcttcc
      601 tctgaaagct ttgtgctttc aagcagaaaa cataaaaatg catatcatca gacgggaata
      661 aaaaattcaa atactgttga aaaagaatga gaaactccgg cattgaatga tctgaaattt
      721 ttcttgagtc ggtgaagaaa atcctcacag aagtgcaagt gccgaaaaca aatgtctcag
      781 tttgtcgcgt agtaacctct gatttttttc agatcgtatt cagtgaaaat tctgtccaga
      841 aactgaagtc tgattttttt accgtttcgg agctgtgctt ccagatttac atagtccatc
      901 caaaggattc gttttttttc cttgaagatc agattatccc aggggatgag gacaggtcct
      961 gcgccgaagc gaaagagcac aaagatatcc atatatagtc ctttttctgt gaagcagata
     1021 tggatgactc cgttgtagct cagccatcca atctgcacat acgaaaaact ttcttccctg
     1081 ccggacggaa ctgtctcggt tccgaaactt tcctgaaggg caaaccatcc gctgacagta
     1141 tgcagaagtt tcatcagtac aacccagaag atgaagaata caaccagtac gccgatgaat
     1201 acaaatat
//
LOCUS       DAOQGJ010001626         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96043_length_1207_cov_2.657986, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001626 DAOQGJ010000000
VERSION     DAOQGJ010001626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96043_length_1207_cov_2.657986"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..>1207)
                     /locus_tag="PK683_20190"
     CDS             complement(10..>1207)
                     /locus_tag="PK683_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10828.1"
                     /translation="HGGTSGYGLDINSSDNNEFINIIAASSGGGGTGGGIKISTSSKN
                     LFYFINAGNNDNNGIQCTGSNSNMYLFVIHTNNRSSFSFGCSNQFAANAVSANSSNFN
                     SSFALPSGAVNNFFLNLGAFNPEGSGFDFTSGSPSYNEFLNFGTLAQGNVNAIGGMTG
                     SSAVFRGNIVIASGKNCAGDGVDGSCNPLSGDAVRFSPASVGTPFLGALKDWSILDTS
                     NSSDALTAVAFGTGMDAYRFDTFYRNWGAYSATNFAASPGVSHEGNCLAGNCTLYSFD
                     MWASQSVFRGINACPESLPLPSLEHKWESAGAANDSDCQLKFPGSYFVAAGDCRSLAL
                     RNAVELIGDSVGNENGICEKYENCIYSPNLGAYQGHGSIVPAASCTSNISSAGINMYQ
                     FQINGY"
BASE COUNT          348 a          287 c          243 g          329 t
ORIGIN      
        1 ttattccttt taatatccgt ttatctggaa ttgatacata ttaattcctg cacttgaaat
       61 gttcgatgta caggacgctg ccggaacaat cgatccatgc ccctgatatg cacctaaatt
      121 tggtgaatag atgcaatttt catacttttc acagattcca ttctcatttc ccacagaatc
      181 accaatcagc tccaccgcat ttcgaagtgc cagacttctg cagtcccctg cggcaacaaa
      241 ataagaaccg ggaaatttca actggcagtc ggaatcattt gctgctcctg cagattccca
      301 tttatgttcg agtgaaggca gaggaagaga ctcaggacag gcattgattc ctctgaacac
      361 tgactgtgat gcccacatat caaacgaata caaggtgcag tttccagcca ggcagttccc
      421 ttcgtgactg actccaggtg aagcagcaaa atttgtagca gaataagcac cccagttgcg
      481 ataaaatgta tcaaatctgt acgcatccat ccctgtccca aacgcaactg cagtcagagc
      541 atcagaactg ttggatgtgt cgagtatcga ccagtccttc agcgcaccca ggaaaggagt
      601 tcctacagaa gcgggagaaa atctgacagc atctccgctc agcggattgc aggaaccgtc
      661 gaccccgtct cctgcacagt tttttccgga tgcaataacg atatttcccc tgaaaacagc
      721 cgatgatcca gtcattcctc ctattgcatt tacattgccc tgtgcaagtg tgccaaaatt
      781 cagaaactca ttataactgg gagaaccgga agtaaaatca aatccggaac cttcaggatt
      841 gaatgcaccg agatttaaaa agaaattgtt tactgcaccg gaaggcaagg caaatgaact
      901 gttgaaattc gatgaattcg ctgaaactgc atttgctgca aactggttgc tgcatccgaa
      961 agaaaatgaa gatctgttgt tcgtatgtat tacaaaaaga tacatatttg aattggaacc
     1021 ggtacactga atcccgttat tgtcattgtt gcctgcattg atgaagtaga acagattttt
     1081 gctcgatgtg ctgattttga tccctcctcc ggttcctccc cctccggacg aagcggcaat
     1141 aatattgata aattcattat tatctgatga atttatatct aatccgtatc ctgatgttcc
     1201 tccgtgt
//
LOCUS       DAOQGJ010001627         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96061_length_1207_cov_2.388021, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001627 DAOQGJ010000000
VERSION     DAOQGJ010001627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96061_length_1207_cov_2.388021"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            404..1201
                     /locus_tag="PK683_20195"
     CDS             404..1201
                     /locus_tag="PK683_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10829.1"
                     /translation="MERIFYFLVGAVLFLAACFGMYKLVGSGEFLDWTVGALSFLWLI
                     FIVTIPWNSYFKAKEILYEAEVSRLKNIAVKEEGVAFAKSVSRRSLIVSISLHIVSAG
                     ALFYIANRGISQVGYYTAGLTILLTFLRPGIRFYEYLHRRLEMIRQEFQYPREDVQEL
                     LSKVQDMIYRTESLEGQLSLDETSNSWRLQLEAQLAQVFEKLQKLDHKLDDVFRSNRE
                     TKNDIESSVAKELESIRKENKDMVAKIVENGEVVESISTIAKYLKKL"
BASE COUNT          366 a          245 c          273 g          323 t
ORIGIN      
        1 atccttgatt tcatcgggag ccgctgattt tacaagggat tcatcggcag ccagccctgc
       61 cttggaagaa tgtctataat tattctataa ttatagacat tctattcagt atccgcgtgc
      121 agaattgagt cccgctgatg aattcagaaa tgctcagcct agcggcagat gcttcaatgc
      181 ttgcgtcgca gttacaaata cccttttcaa gcacttgttt taaaaaagca agaaaaggtt
      241 ggtccctaag ccatcaaact gtcggcagac cttcctgcca aaatgcatca cctgcacccg
      301 ttaaactgag ttcttaaaaa ggaaatagga taaaaatatg cttgaatgcc caatgagatt
      361 cctgtacaag taattctatc tttaaatact gatggagaaa tgaatggaaa gaatttttta
      421 ttttctggta ggcgctgtcc tgtttctggc agcctgtttc gggatgtaca aactggtcgg
      481 gtcaggggaa ttcctggact ggacggtcgg agcactgtcc tttctctggc tgatcttcat
      541 cgtgacaatt ccctggaatt cctattttaa agcgaaggaa atcctgtatg aagcagaagt
      601 ttccagactg aagaacattg cagtgaagga agaaggcgta gcctttgcaa aatccgtcag
      661 cagaagatct ctcattgttt ccatctcact gcacattgta tctgctggag cattatttta
      721 tattgcaaac agaggaatca gccaggtagg ttactacact gcaggcctta caatccttct
      781 gactttcctg cgccccggaa tcaggtttta cgagtatctg cacagaagac tggaaatgat
      841 ccgtcaggaa tttcaatatc ccagagagga tgttcaggaa cttctttcca aggttcagga
      901 tatgatttac agaacagaaa gcctggaagg acagctgagt ctggacgaaa catcaaattc
      961 ctggagactt cagctggaag cacagcttgc acaggttttt gaaaaacttc agaaactgga
     1021 tcataagctg gatgatgttt tccgctccaa cagagagact aagaatgaca tagaatcttc
     1081 cgtagccaaa gaacttgagt ccattcggaa agagaacaag gatatggtgg cgaagattgt
     1141 ggaaaacgga gaagtggtag aaagtatttc gacaatagca aagtatctga aaaagctgta
     1201 aaaatgc
//
LOCUS       DAOQGJ010001628         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96080_length_1207_cov_2.171875, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001628 DAOQGJ010000000
VERSION     DAOQGJ010001628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96080_length_1207_cov_2.171875"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PK683_20200"
     CDS             <1..592
                     /locus_tag="PK683_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10830.1"
                     /translation="MKTSAESKKNAGFVLAWTLGLANARESAIEKIPGLEDQCFSLIF
                     GFSCSDNEKKPFSEYLSESPWNHPSAFGKTANKEIVFRRFGKAKGLDGGKFTGNPVPF
                     IHQDRIYVYDGENYFRLYSDIFGTVIEAAEKVKSSPSASGRFQWRNGKLSYEGVSADI
                     PEFPTSRTGMTCSEGTAVFFSAYSFSLLIAGVKKYA"
     gene            585..>1207
                     /locus_tag="PK683_20205"
     CDS             585..>1207
                     /locus_tag="PK683_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH10831.1"
                     /translation="MPKMTESELIEKWNEKWEDALALWSRFTKLKDPFYFTGESGKKK
                     NKTSEDYSDTLAMILLDEHKVVINTKLVFHYGLEDFPMEILGHEIGHHVYCPGDLTGH
                     GRMLGRVGKALADQKQHAPFISNLYSDLMINNRLKQNHGLRMEEVYKKLNQKKQDSLW
                     QLYMRTYEILWALESETLAFGVSEKTIEADAQLCNRIIRSYAEDWLFG"
BASE COUNT          366 a          251 c          288 g          302 t
ORIGIN      
        1 aatgaagact tctgcagaga gcaaaaaaaa tgcaggcttt gtcctggcct ggaccctcgg
       61 gctcgcaaac gcaagagaat ctgcaattga aaaaatcccc ggcctcgaag atcagtgttt
      121 ttcactcata ttcggatttt catgctctga taatgaaaaa aaaccttttt ctgaatatct
      181 ctctgaaagc ccgtggaatc acccttccgc attcggcaaa actgccaaca aggaaattgt
      241 attccgcaga ttcggaaaag ccaagggact tgacggcgga aagtttacag ggaatccagt
      301 gcctttcatt catcaggaca gaatctatgt ctatgacgga gaaaattatt ttcgtctata
      361 ttctgacata ttcggaacag tgattgaagc ggctgaaaaa gtaaaatcat ccccttccgc
      421 atcaggcagg tttcagtgga gaaacggaaa actttcctat gaaggtgttt ctgcagacat
      481 tcctgaattt ccgacgagca ggacaggaat gacatgttcg gaaggcactg ccgttttctt
      541 ttctgcatac agtttttcac ttctgattgc gggcgtaaaa aaatatgcct aagatgactg
      601 agtcggaact cattgagaaa tggaatgaga aatgggagga tgcacttgct ctttggagca
      661 ggttcactaa actgaaggat cctttttatt tcacggggga atcaggaaag aaaaagaaca
      721 agacttctga agactacagc gatactcttg caatgattct tctggatgag cacaaggtgg
      781 tcatcaatac aaaacttgta tttcattacg gacttgagga tttcccaatg gagatcctgg
      841 ggcatgagat cggacaccat gtgtactgtc cgggggatct gaccggacac ggaagaatgc
      901 tcggccgggt tggaaaggca cttgcagatc agaagcagca tgcacctttc atttccaatc
      961 tttattcaga tcttatgatc aacaacaggc tgaagcagaa ccacggactg aggatggagg
     1021 aagtgtataa aaagctcaac cagaagaaac aggacagtct ctggcagctt tatatgcgaa
     1081 cttatgaaat tctctgggca cttgaaagcg aaacccttgc attcggggtc tccgaaaaga
     1141 caattgaggc agatgctcag ctctgcaaca ggatcatcag aagttatgca gaggactggc
     1201 ttttcgg
//
LOCUS       DAOQGJ010001629         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96154_length_1206_cov_4.596003, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001629 DAOQGJ010000000
VERSION     DAOQGJ010001629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96154_length_1206_cov_4.596003"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK683_20210"
     CDS             complement(<1..314)
                     /locus_tag="PK683_20210"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNH10832.1"
                     /translation="MSVYSAAVTLLLVMDPFGNIPVFLSVLNSCDPKRRKLIIIREMF
                     IALFLLSVFLFFGQYILSGMHISEPALSISGGIVLFLISIKMIFPDKSEHTEQSFEKE
                     PV"
     gene            547..924
                     /locus_tag="PK683_20215"
     CDS             547..924
                     /locus_tag="PK683_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10833.1"
                     /translation="MTKQILLFSVFLCSFSVFSSDHIECFFEAKIDSVSGSAVSSKRE
                     KKMQSQKTSFEILKVSKASEENFCREMKKKKKYDAEVLFKNGQTLKAGKKVILKFEYT
                     ASEVVKNGKVADMVEYEAWYLID"
BASE COUNT          415 a          219 c          231 g          341 t
ORIGIN      
        1 atcacaggct ctttctcaaa actctgctca gtatgttccg acttgtccgg aaaaatcatt
       61 ttaatggaaa tcagaaacag gacaatcccg cctgaaatgc tcagggcagg ttcagagata
      121 tgcatgcctg atagaatgta ctgaccgaaa aaaagaaaga cagaaagcag aaacagtgca
      181 atgaacattt ccctaatgat gatcagcttc cttctttttg gatcacagct gttcagcaca
      241 gagagaaata caggaatatt tccaaaaggg tccataacaa gaagcagggt gactgcggca
      301 gagtagactg acatatttat tctttgataa atctattatc taaaaggtca atggaaatac
      361 ataacctgac ttagagcaca ggaatggttg acgatcatga actcaaagag ataattctcc
      421 ttttgtaatt ttcagtaagc agttactcag tacagacaaa ttcatccatg cctggctcgg
      481 taaaaaaccc cttccgctgt gatagaaaaa tcttgaaaaa atctgccaaa ctgtttcagt
      541 gaagaaatga caaaacaaat ccttctgttt tccgttttcc tctgttcgtt ttccgttttt
      601 tcttccgacc atatcgaatg tttctttgaa gcaaagattg actccgtttc cggttcagcc
      661 gtttcctcaa aaagggaaaa gaaaatgcag tctcaaaaga ccagtttcga aatactgaaa
      721 gtttccaagg cttccgagga aaacttctgc agagaaatga agaaaaagaa aaaatatgat
      781 gcggaagttc tgtttaaaaa tggacagacc ttaaaggcag ggaagaaagt aattctcaaa
      841 tttgaataca cagcctcaga agtcgtcaaa aacggtaaag ttgcggatat ggtcgaatat
      901 gaagcctggt acctgattga ttaggggaga aagagatctt tttcattgcc ctcaaaaacc
      961 ttgatgctca gtagtattgt ttgatatatt cataagcttt atcaaagagc gactgatctt
     1021 tatgaagctt atattttatc ttccgatcat attaaatgtt ttcttgaagc aaagatagat
     1081 ttcctgtaaa gttcggttat acaaacggtt ggaaactgat ctggaattcg aattgacaat
     1141 gtacacgtcg cgatttcaca ataattctat gaattacaaa acaataagat tgaaaacata
     1201 tttggt
//
LOCUS       DAOQGJ010001630         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96164_length_1206_cov_3.779322, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001630 DAOQGJ010000000
VERSION     DAOQGJ010001630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96164_length_1206_cov_3.779322"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PK683_20220"
     CDS             <1..305
                     /locus_tag="PK683_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10834.1"
                     /translation="YGNGVFVTLAFGSTNAATSPDGISWTARTLPNSSNWFSVAYGNG
                     VFVALASNSTVAATSTDGITWTARTLPSSVNWSSVAYGNGIFAAISIGGTTAATSP"
     gene            392..1159
                     /locus_tag="PK683_20225"
     CDS             392..1159
                     /locus_tag="PK683_20225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH10835.1"
                     /translation="MNKFWFENSDFWDLLYSLFFAENRADPCSDTEHLIQLTGISPPA
                     EVLDIACGSGRHSIPFARRGFKVTGIDITESYLHRAENFAVESGENIEFIRSDMLHFS
                     RQSAFDLIIVLYSSFGFFAEQNDDSKFLENMFDSLNRNGQLILDLKNREYVRDNFIAE
                     SNMNIDGITYSEFREVEGNFDLIHTTWTIQSSEFSKTFRTSIRLYDAETVQILLNDAG
                     FGCCTLYSSFKGDLFEKKSPRICAAAGKGKSIGPFRI"
BASE COUNT          337 a          253 c          264 g          352 t
ORIGIN      
        1 catacgggaa cggtgttttt gttactctgg ctttcggatc aacaaacgct gcaacttctc
       61 ctgacggaat ttcctggact gcaaggacac tgccaaattc atccaattgg ttttccgtgg
      121 catacgggaa cggtgttttt gttgctttag catccaattc aacagtcgcg gcaacttcaa
      181 cagatggaat cacctggact gcaagaacat tgcctagttc tgtaaactgg tcatcagtcg
      241 cttacgggaa cggtattttt gcagctatat caatcggggg aacaacagca gcaacttctc
      301 cttaaaaatc tttttcaata gagaccgctg aatctttcaa ataatttgaa atcatttact
      361 gtttttgaaa actgtgttca gaagctgcat tatgaacaaa ttctggtttg aaaattcaga
      421 cttttgggat ctgctctatt ccctgttctt tgccgaaaac agagcagacc cctgttccga
      481 tacggagcac ttgattcaac ttactggaat cagtccgccg gctgaagttc tggatattgc
      541 ctgcggatcc ggaaggcatt ctattccttt tgccagaagg ggcttcaagg tgacgggtat
      601 tgacattact gaatcatatc ttcatcgtgc tgaaaatttt gcagtcgagt cgggtgagaa
      661 catagaattc atcaggtcgg atatgcttca cttttcaaga cagagcgcat ttgatctgat
      721 cattgtactt tattcctctt tcggcttttt cgcagaacag aatgatgaca gtaaatttct
      781 tgaaaatatg ttcgactcat taaacaggaa cggtcagctt atacttgatc tgaagaacag
      841 ggaatatgtt cgggataatt ttattgcaga aagcaatatg aacatcgatg gaattacata
      901 cagtgaattc cgtgaggtgg aaggaaattt tgatctgatt catacaacgt ggacaattca
      961 gagttcagag ttctccaaaa cctttcggac aagcatccgt ctttacgatg cagaaacagt
     1021 tcaaatcctt ttgaatgatg caggattcgg atgctgcacc ctctacagca gtttcaaggg
     1081 tgatctattt gagaagaaat ctccccgcat ctgcgctgca gccggaaagg gcaaaagcat
     1141 cggtccattc cggatttgat gtctcgttaa aaacaggctc cgcactcaga aaagaagcgg
     1201 ggcatt
//
LOCUS       DAOQGJ010001631         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96206_length_1206_cov_2.606429, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001631 DAOQGJ010000000
VERSION     DAOQGJ010001631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96206_length_1206_cov_2.606429"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PK683_20230"
     CDS             <1..504
                     /locus_tag="PK683_20230"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNH10836.1"
                     /translation="PVSGKEYMNVQIFVPVSNLIGFLKSVQKIGNVNSETINTEDHTE
                     ANELQKIVMEREEQRGRRRNKAVSSASSANWNWKDREDALERSEDGFDHAKLEKWKIQ
                     DKVSFAKVSIQITGKKVESDFELLKAVKKGWMTVLEFLEALISIWPFLILGLILFWYF
                     RYRRNRI"
     gene            complement(557..>1206)
                     /locus_tag="PK683_20235"
     CDS             complement(557..>1206)
                     /locus_tag="PK683_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10837.1"
                     /translation="TAPTSAPWAPCTGGTSLDTSNNLFTQNSYKIFSIIGLNSSTGQP
                     GTCTGTGGSSVSASNANNGYANYFNLSNATGGSVASICSADFSPIMTSIVTEAAATGS
                     PYVLSKAPISSTIAVKVAGASVTQNASNGWMYDSATNSIIFSGSAWPAVGAAIEVTYE
                     YNSSAAAMNSGASQTLTAFIGNAVSSDAGKIGLGVLVLAGLAVFGRNYFSRKNQA"
BASE COUNT          357 a          232 c          290 g          327 t
ORIGIN      
        1 cctgtttcag gaaaggaata tatgaatgtt cagatatttg taccggtttc caacctgatc
       61 ggttttctga agtcagtgca gaaaattgga aatgtaaatt ctgagacaat caatacagaa
      121 gatcatacgg aggcgaacga actgcagaag attgtaatgg agagggaaga acagaggggc
      181 agaagaagaa acaaagccgt cagttctgca tccagtgcca actggaattg gaaggacagg
      241 gaagatgctt tggaaagaag cgaggacgga ttcgatcatg cgaaactgga aaaatggaaa
      301 atccaggaca aagtgagttt tgcaaaggtt tccattcaga ttactggaaa gaaagtggag
      361 tctgattttg aacttctgaa ggcagtcaag aaagggtgga tgactgtcct tgaattcctc
      421 gaagctttga tttctatctg gccctttttg attctcggac tgatcctatt ctggtatttc
      481 aggtacagaa gaaacagaat ataaaaaaaa ggcatggaga atatccatgc ctctgcattg
      541 ttatggagat ttttgcttac gcctgatttt ttctgctgaa gtagtttctt ccaaaaacag
      601 ctagtcctgc gagaaccaat actcccaatc ctatttttcc tgcatcactg ctcactgcat
      661 taccgatgaa tgctgtcaga gtctgagaag ctcctgaatt cattgcagca gcggagctgt
      721 tgtattcata agtcacttca atagcagctc ctacagcagg ccaggcagat ccggagaaaa
      781 tgattgaatt tgtagctgaa tcatacatcc atccattgct tgcattctgt gttacggatg
      841 ctcccgcaac tttcactgca atagtggaag atatcggagc tttagaaaga acataagggc
      901 ttcctgttgc tgcagcctca gtgactatgc ttgtcatgat tggactgaaa tctgccgaac
      961 agatagaagc tacagatccg ccagtcgcat tcgaaagatt aaaatagttt gcataaccgt
     1021 tatttgcatt tgatgctgat acggatgacc caccagttcc ggtacaggta ccgggctgtc
     1081 cagttgagga atttaagccg attatgctga aaatcttgta gctgttctga gtgaacaggt
     1141 tattcgatgt atccaatgaa gttccgcctg tacatggtgc ccaaggtgca gaagtaggag
     1201 ctgtgt
//
LOCUS       DAOQGJ010001632         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96475_length_1204_cov_3.154917, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001632 DAOQGJ010000000
VERSION     DAOQGJ010001632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96475_length_1204_cov_3.154917"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PK683_20240"
     CDS             <1..863
                     /locus_tag="PK683_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNH10838.1"
                     /translation="GLYDTVLVLGVEKMNTRDSETTMQIVSRGQDFEFEGGFCVSGPS
                     GFAMNAMRHMHDFGTTKEMLSTVAEKNYFHGSKNPYAHKQKVIPLKNIMNARTVTTPF
                     GFHDMSLVTDASAAVVLTTKERAKSISKKPVIVKGSGHGGDYFTLSLKNSSTSFPATV
                     KAAEEAFKMADVKRSDIDVLECHDCFTITEIINIEDLGFVEKGKGGYYTMEGHTRLGG
                     KLPVNTSGGLKAKGHPIGATGVGQVVEMTFQLREEAEARQVKNARTAMTHVLGGPGAV
                     SIVHILQRGY"
     gene            863..1201
                     /locus_tag="PK683_20245"
     CDS             863..1201
                     /locus_tag="PK683_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNH10839.1"
                     /translation="MEAVMEKLVGKKCNSCGQHMLNPSFACANCGSDAIDVFEFKGTG
                     EIYTFTVVMVGFGHLAPRAPYVLAIVSLEEGLKVLTIVEDLDKDKAAIGIKVKFKRWE
                     EGTGPIFTVL"
BASE COUNT          358 a          223 c          316 g          307 t
ORIGIN      
        1 caggacttta tgatacagtt cttgtgctcg gtgtggaaaa aatgaatacc cgcgattctg
       61 aaactacaat gcagatcgtg tcgagaggtc aggactttga atttgaagga ggattctgtg
      121 tgtcgggccc ttccggattt gcaatgaatg caatgcgcca tatgcatgat ttcgggacga
      181 caaaggaaat gctttccact gtggctgaga aaaattattt tcacggaagt aaaaatccgt
      241 atgcgcacaa acagaaagtc attcctctga aaaacataat gaatgcaaga acagtgacga
      301 ctcctttcgg ttttcatgat atgtctctag tgaccgatgc ttctgcagca gtggttctga
      361 cgactaagga aagggcaaag tccatttcca aaaaacctgt catcgtgaaa ggttcaggtc
      421 acgggggaga ttattttact ctttcgctca agaacagttc cacctcattt cctgcaaccg
      481 tcaaggcggc agaagaagca ttcaaaatgg ctgatgtgaa aagatcggat attgatgttc
      541 tggaatgcca tgactgtttt actatcactg agatcattaa tatagaagac ttaggatttg
      601 tggaaaaagg gaagggcggc tattatacaa tggaaggtca tacaagactc ggaggaaagc
      661 ttcctgtaaa cacttctgga ggactcaagg caaaaggtca tccgatcggt gcgacaggag
      721 tcggtcaggt agtggaaatg acttttcagc tccgtgaaga agcggaggct agacaggtga
      781 aaaatgcaag gactgcaatg actcatgttc tgggcggacc gggagcagtc agcatagttc
      841 atatacttca gagggggtac tgatggaagc tgtaatggaa aaactggtcg gaaaaaaatg
      901 caattcctgc ggtcagcata tgctgaatcc cagctttgcc tgtgcaaact gcggaagtga
      961 tgcgattgat gtttttgaat tcaaaggaac gggtgagatt tatacattca ctgtagtgat
     1021 ggtaggtttc ggtcatcttg cacctagagc tccctatgtt cttgcaattg tatctcttga
     1081 agaaggcctc aaggttctga ccatcgtgga agatctggac aaggacaagg cggcaatcgg
     1141 gatcaaggtg aaattcaaaa gatgggaaga aggcactggc ccgatattta cagtgttgtg
     1201 aaat
//
LOCUS       DAOQGJ010001633         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96612_length_1203_cov_4.288328, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001633 DAOQGJ010000000
VERSION     DAOQGJ010001633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96612_length_1203_cov_4.288328"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1203
                     /locus_tag="PK683_20250"
     CDS             <1..>1203
                     /locus_tag="PK683_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10840.1"
                     /translation="TKIGAVGIPKTVSTLELKPYGYFDDVTGQYVFTKGQVILMVPYG
                     TLFDNINLEVETKNNTKKVSADGKDYSRGNTFVKFMQRNGSEKYTVDAVLTDKYSAFE
                     VPYEIQIVMGAPPPFVAVSKDSTFMLFVNSTAASTRDESNELAEIIRWFAPFYNAGSA
                     ISDLKTVLAKVNEVKISVARWTVDPGKVDLNIIGQGFKGYDFDVESYEEDLKCFKYPY
                     AVYESDSKFRWGADELYYYANLETIHRNMHKEYCGKSKGNETYPSYVKKYPRISSYGR
                     ILNNAKTVEGYGIKAEDLNQEMDSAFFAKTLTSEVPKMNQNGSAFYLQPVRGEKVLYE
                     LPAVNYIEKMEPNNGTVGTEVLVTFAAPLEDNTYTISCTMDNTPVLNLKVRDSRSVYF
                     NVPNISKGT"
BASE COUNT          382 a          236 c          288 g          297 t
ORIGIN      
        1 cacaaaaatc ggtgcagtcg gtatacctaa aacagtatcc actttggaac tgaaacccta
       61 cggatacttt gatgatgtca ccggtcagta tgtattcaca aagggccagg tcatacttat
      121 ggtgccttac ggaacacttt tcgacaatat aaatctggaa gtggaaacga aaaacaatac
      181 gaagaaggtt tctgcagacg gaaaggatta ttcaagagga aacacattcg taaagtttat
      241 gcagagaaac ggaagtgaga aatacactgt ggacgctgtt ctgaccgaca agtacagtgc
      301 atttgaggtt ccgtacgaaa ttcagattgt catgggcgct cctcctcctt ttgttgcggt
      361 atcgaaggat tccacattta tgctctttgt aaattccact gctgcaagca caagagacga
      421 atccaatgaa ctggcagaga ttatccgctg gtttgctccg ttttacaatg caggatcggc
      481 aatcagcgac ctgaagactg ttcttgccaa ggtcaatgag gtgaagattt ctgttgcgag
      541 atggacagta gatccgggga aagtggatct caacatcatc gggcagggat tcaaaggata
      601 tgattttgat gtagagtctt atgaagagga tctgaaatgt ttcaagtacc cttacgctgt
      661 atatgagagc gacagcaagt tcagatgggg tgctgatgag ttatactact atgcaaacct
      721 ggaaacgatt cacaggaata tgcacaagga gtactgcgga aaatccaaag gaaatgaaac
      781 atatcccagt tacgtgaaaa aatatccccg gatctccagt tacggcagaa tactgaacaa
      841 tgcaaaaacc gttgaaggat acggaatcaa ggcagaagat ctgaatcagg aaatggattc
      901 tgcatttttt gcaaagactc tgactagtga ggttccgaaa atgaatcaga acggcagtgc
      961 tttttatctt cagccggtca ggggagaaaa ggttctttat gagcttcctg cagtgaacta
     1021 cattgagaaa atggaaccga acaacggaac agtaggaaca gaagttcttg tcactttcgc
     1081 cgcaccgctg gaagacaata cgtacactat ttcctgtaca atggacaata cacctgtgct
     1141 gaatctcaag gttcgggaca gcagaagcgt ttattttaac gtgccgaaca tttccaaggg
     1201 aac
//
LOCUS       DAOQGJ010001634         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96784_length_1202_cov_3.393200, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001634 DAOQGJ010000000
VERSION     DAOQGJ010001634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96784_length_1202_cov_3.393200"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK683_20255"
     CDS             complement(<1..268)
                     /locus_tag="PK683_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNH10841.1"
                     /translation="MKQDSAALQTKKASFRAKENTVCPICNHTFQREQMFQGGGRLIA
                     GNLTKELRRLYQKNKKFGRINPNDYLMVTCSRCLYTAYPRDWPSL"
     gene            complement(317..>1202)
                     /locus_tag="PK683_20260"
     CDS             complement(317..>1202)
                     /locus_tag="PK683_20260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNH10842.1"
                     /translation="KETNTQAFLLAKDGTILQEKYFKGKTEESVFPSYSIAKSIVSLL
                     FGTVMINDRKIFSEEDFAWKYIPEYKNLKGIRMHDFLNMTSGIIFQENYLSVLSDFYL
                     FSLSSDLGNRLHSYSAPLKPGMIFEYRSVNTFIIGLIIERVTNQRLSVLLQERIWKKI
                     GTEWDAGWSLDREDGLEKSYCCLNARARDFLKIGRLVLQNGKWENAEIIPKKWMDKLN
                     HIETVEPDTKFGYNRQWWYPGTDPNMICGIGIHGQYLCINRSKKLIFVKFSEHEAEAD
                     EKLTLQAFEKISETLTEN"
BASE COUNT          296 a          262 c          242 g          402 t
ORIGIN      
        1 tcaaggaagg ccagtctctg ggataagcgg tatacaggca tcttgagcag gtcaccatca
       61 gataatcgtt tggattgatt cgtccaaatt tcttattttt ctggtaaagt cttctcaatt
      121 ccttagtaag gtttccggcg ataagccttc ccccaccctg gaacatctgt tccctctgaa
      181 aagtatgatt gcaaatcggg catactgtgt tttcttttgc ccggaaagat gctttcttgg
      241 tttgtagagc tgcagaatcc tgtttcatac gacttaaaga actagtacaa aacaggccag
      301 aattgtcaat tgattcctaa ttttcagtca aggtttcgga aattttttca aaggcctgta
      361 gagtgagttt ttcgtccgct tcggcttcgt gttcgctgaa ttttacaaat atcagttttt
      421 tggaacggtt tatgcaaaga tactgcccgt gaattcctat tccgcaaatc atattcggat
      481 ctgttcccgg ataccaccat tgtctgttgt agccgaattt tgtgtccggc tcgactgttt
      541 caatatgatt gagtttatcc atccattttt tggggatgat ttctgcattt tcccattttc
      601 cattctgcag aaccagtctg cctatcttaa ggaaatctct ggctctggca ttcaggcagc
      661 agtaggattt ttccagtcca tcctctctgt ccaggctcca gcctgcatcc cactccgtgc
      721 ctattttttt ccagattcgt tcctgaagaa gcactgatag tctctgattt gtcactcttt
      781 ctataatcag tccaattata aatgtattga cgcttctgta ctcaaatatc attcccggtt
      841 tcagcggagc tgaatatgaa tgaaggcggt ttcccaggtc gctgctgaga gagaaaagat
      901 aaaaatcgga gaggacactg agatagttct cctgaaaaat gattccggag gtcatattta
      961 aaaaatcatg cattcggatt cctttcagat tcttatactc aggaatgtat ttccaggcaa
     1021 aatcctcttc cgagaaaatt ttacgatcat ttatcattac tgtaccgaaa agcagagaca
     1081 cgatggactt tgcaatagag taggagggga aaacgctttc ctctgttttt cctttgaagt
     1141 atttttcctg cagtattgtc ccgtcttttg ccagcaggaa tgcctgcgtg tttgtctctt
     1201 tc
//
LOCUS       DAOQGJ010001635         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96801_length_1202_cov_3.062772, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001635 DAOQGJ010000000
VERSION     DAOQGJ010001635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96801_length_1202_cov_3.062772"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1202
                     /locus_tag="PK683_20265"
     CDS             <1..>1202
                     /locus_tag="PK683_20265"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH10843.1"
                     /translation="SRYMFREAVEKKFKDQMETVNSGIRKDGKLYLLSLRLVPAFPFF
                     LINLLMGLTEISLGSFYAVSQIGMIPGTFVYVNAGTQLVSLNSPKDILSPVLMISFAA
                     LGMLPIAAKFFLSFLQNRRLYANYKKPDKFDYNIAVIGAGAAGLVTSYISAAVRAKTL
                     LIEKNKMGGDCLNTGCVPSKTLLSAAKKIHLAKKAEQYGLNSAKIDFDFSRIMERVHS
                     VIRKIEPNDSIERYSGLGVECIRGDAKLLSPFEIQIGERKITAKNIVIASGAEPFIPD
                     IPGLKDVGYLSSETLWNLKKLPKTFLVAGAGAIGCELAQAFSRLGSKVVLVEAAERIL
                     PSEDAKLSSFLYEVFIEEGIEIIASGKILQFEKNILGKKAKIQCNDKINEIFFDEVLI
                     CTGRKART"
BASE COUNT          363 a          229 c          272 g          338 t
ORIGIN      
        1 atccagatac atgttcagag aagcagtgga gaaaaaattc aaggatcaga tggaaacggt
       61 caattcagga atacgcaagg atggaaaact ttatctgctt tcactcagac ttgttcctgc
      121 ttttcctttt tttctcatca atcttctgat gggactgact gagatttcac tcggttcctt
      181 ttatgcagta agccagatag ggatgattcc cggaactttt gtctatgtaa atgcaggaac
      241 tcagcttgtt tctctgaatt caccgaagga tatactttct cctgttttaa tgatttcttt
      301 tgcggcactg gggatgcttc ctattgctgc aaaatttttc ctgagttttc ttcagaacag
      361 aagactttat gcaaactata aaaaacctga taaatttgat tataatatag cagtcatagg
      421 tgcaggcgcg gcagggcttg tcacctctta catcagcgca gctgtcagag caaagactct
      481 gctcattgag aagaataaaa tgggagggga ctgtctgaat acaggatgcg tgccttccaa
      541 gactctgctc tctgcagcaa agaaaattca tctggcgaaa aaggcggaac agtacggact
      601 caattctgca aaaatagatt ttgatttttc cagaatcatg gaaagggttc attccgtgat
      661 ccgaaaaatt gaaccgaatg actcaattga aaggtattcc ggactgggag tggaatgcat
      721 tcggggagat gcaaaacttc tctcaccttt tgaaattcag ataggtgaaa ggaaaatcac
      781 tgcaaaaaac attgtaattg cttccggagc ggaaccgttc ataccggaca ttcccggcct
      841 gaaagatgtg ggttatttaa gttcagagac tctctggaat ctgaaaaaac ttcctaagac
      901 ttttcttgtt gcaggggccg gggcgatcgg ctgcgaactg gctcaggcgt tttcccgact
      961 tggatcaaag gttgtacttg tcgaagccgc cgaaagaatt cttccttcgg aagatgcaaa
     1021 actgtcttct ttcctttatg aagttttcat cgaagaagga atagaaatca tcgcctcagg
     1081 aaaaattctt cagtttgaaa aaaacatcct gggaaaaaag gcaaagatac agtgtaatga
     1141 taaaataaat gaaatatttt ttgatgaggt gctgatatgc actggaagaa aggcgaggac
     1201 cg
//
LOCUS       DAOQGJ010001636         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_96974_length_1201_cov_3.127400, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001636 DAOQGJ010000000
VERSION     DAOQGJ010001636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96974_length_1201_cov_3.127400"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1201)
                     /locus_tag="PK683_20270"
     CDS             complement(<1..>1201)
                     /locus_tag="PK683_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10844.1"
                     /translation="ETDSISDTFEKLNLLKRKEEILNQIHSEEKKVRDIGENKEKILS
                     SVKCRNSDDISFIIKREKKNAEDLQKKINSLKVRIELKNYSENLKEGEPCPVCGSTFH
                     DISVLHSENITDELHYNEKLLHESEKLNSEAEKAERVLENLNKETEIHSKIINDLKKN
                     FGEESDEKQLKSGIQELQNHYSIQKKKKDENSFKEKHLQTQEKKLEENREAARREEKI
                     ESEQSAQITVLASAAEFNRKNVTDEVFLKYGSLGTEKTAELILDLEKKKTEAEKKFTA
                     EEDIIKKREEKQSSLRGRKESEEENLSDMEKRFSRLKKEYEEKIKVHDTSEEEILKSL
                     SFQNAEKEKEEIEKFFQSKKNKEAVLKSLEMVESVPPERRIISGMKEAVVTMENEISA
                     LDKEAGTL"
BASE COUNT          239 a          249 c          184 g          529 t
ORIGIN      
        1 agtgttcctg cttccttgtc cagtgccgat atctcatttt ccatagtgac aacggcttct
       61 ttcatccctg agataatcct cctttcagga ggaactgatt ccaccatttc aagacttttg
      121 agaactgcct ctttattttt cttggactga aagaattttt cgatttcctc tttttccttt
      181 tcagcattct gaaaagagag gctcttcagt atttcttcct cagaggtatc atgaactttt
      241 attttttctt catattcctt tttcagtctg gaaaaccttt tttccatatc agaaagattc
      301 tcttcttctg attcctttct tccgcgaaga ctgctttgtt tttcttctct tttttttatg
      361 atatcctctt cagctgtgaa ttttttttca gcttcagttt tctttttttc cagatcaaga
      421 atcagttctg ctgttttttc tgttccgagt gaaccgtatt ttagaaagac ttcgtctgtg
      481 acattttttc tgttgaattc tgcggcactt gccagaacag taatctgtgc ggactgctct
      541 gattcaattt tttcctctct tcttgctgct tcccggttct cttccagttt tttttcctgt
      601 gtctgaagat gtttttcctt gaaagaattt tcatccttct tctttttctg aatgctgtag
      661 tgattctgaa gttcctgaat gcctgatttc agctgctttt catcggactc ttcaccaaag
      721 ttttttttca gatcatttat tatttttgaa tgaatttcgg tttctttatt cagattttcc
      781 agaactcttt ctgccttttc tgcctctgaa tttaatttct ccgattcgtg aagcaatttc
      841 tcattataat gtaattcatc tgtgatgttt tctgaatgca gaaccgatat gtcatggaaa
      901 gttgagccgc agacagggca gggttctcct tctttcagat tttcagaata gtttttcagc
      961 tctatcctga ctttcagact gtttattttt ttctgaagat cctctgcatt tttcttttct
     1021 cttttaatga tgaaagatat atcatctgaa tttctgcatt ttacagagct cagaattttt
     1081 tctttatttt ctccgatatc tctgactttt ttttcttcag agtgtatctg attcagaatc
     1141 tcttcctttc ttttcagaag gttcagtttt tcgaatgtat cggatatgct gtctgtttct
     1201 g
//
LOCUS       DAOQGJ010001637         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97008_length_1201_cov_2.395288, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001637 DAOQGJ010000000
VERSION     DAOQGJ010001637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97008_length_1201_cov_2.395288"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PK683_20275"
     CDS             <1..255
                     /locus_tag="PK683_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10845.1"
                     /translation="GELKVNLAFKVNKGMNVIRLIQRNDKGERSGLVLYTGINYSGQG
                     KVSDLFLKRIKLNRILSPLSVNGRFQILTETDLKKMDIEN"
     gene            490..>1201
                     /locus_tag="PK683_20280"
     CDS             490..>1201
                     /locus_tag="PK683_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10846.1"
                     /translation="MYFRRGRVYELKRIHSVLVLLLIAGASYLIPFLQKNETGFYSRG
                     GGDHSTYLTLSEWFVSHSLWETGKADQVNPPKVRWELNRFLDDRKGFIADFPSKNLQP
                     LANQMISTPYMAVFSGYSEETYTASVAFYLSISALSYILFFSSFFHLTGFSVYAIGTA
                     LSLSSMLMYVATCHSIPFLYSIALFNYLIYFSLSVGFTADTEWKRYFGAVLTGGCLLM
                     IYPHLWILFLFLLLPVYFF"
BASE COUNT          302 a          225 c          255 g          419 t
ORIGIN      
        1 ggggaactga aagtaaattt ggcctttaag gtaaacaaag gtatgaatgt tatcaggctg
       61 attcaaagaa acgacaaagg cgaaagaagt ggcttggtat tgtacacagg aattaattac
      121 agtgggcaag gtaaggtttc tgatttattt ttaaagagaa ttaaactaaa ccggattctt
      181 tctccccttt ccgttaatgg aaggtttcag attttgactg aaactgattt aaaaaaaatg
      241 gacattgaga actgaattat gaatgcagca gttctttttt taaaacttgc gggaattttt
      301 tctctttctt gggtaagtgg ctggtctttg cagttcagca gtcagacgga gagctcagat
      361 ctggtttccg atataaagaa tttgattctg ggatcagcgt tcctccttgt atcggttatt
      421 ttatcgacag tattgggatt gcccggagca gcaggacttc ttcctccggt attaatcttt
      481 ctgattaaga tgtatttcag aaggggacgc gtttatgaat taaagagaat ccactcagtt
      541 cttgtccttc tgcttattgc cggagcctct tatctgattc cttttcttca aaaaaacgag
      601 acgggtttct attcccgagg tggcggtgat cacagtacgt atctgactct ttcagaatgg
      661 ttcgtcagtc acagtttatg ggagacagga aaggcagatc aggttaatcc tccgaaagta
      721 aggtgggaac tgaaccgttt tctggatgac cgaaagggat ttatcgcgga tttcccttca
      781 aaaaatttac agcctctggc caatcagatg atatccactc cgtacatggc agtattttct
      841 ggatattcgg aagaaacata tactgcatct gtggcttttt atctgtccat atccgcactg
      901 agttatattc tgttcttctc ttcgttcttt catctgacag gattttctgt atatgctatt
      961 ggaacagcac tgtctctgtc ttctatgctg atgtatgtag ctacctgtca ttcaatacca
     1021 tttctgtaca gtatcgccct ttttaattat ctaatctatt tcagtctttc tgtcggtttc
     1081 actgcggata ctgaatggaa acgttatttt ggagctgttc ttactggcgg atgtcttctg
     1141 atgatttatc cgcatctttg gattttgttc ttattcctgc ttcttcctgt atacttcttc
     1201 t
//
LOCUS       DAOQGJ010001638         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97038_length_1201_cov_1.971204, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001638 DAOQGJ010000000
VERSION     DAOQGJ010001638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97038_length_1201_cov_1.971204"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PK683_20285"
     CDS             complement(<1..468)
                     /locus_tag="PK683_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNH10847.1"
                     /translation="MLFNSLEYFIFFSAVFIYQWYLHPLLFSGRENSRQILHSFLLGA
                     SYIFYMSWDWRFSGLIFLSTLIDYVLALKIDAEEDSAARLAWLRLSLFLNLIVILGFF
                     KYYNFFASSSNSLLSLLGLGSPVPVIKNLILPVGISFFTFQSMSYTIDVYRRHI"
     gene            complement(564..869)
                     /locus_tag="PK683_20290"
     CDS             complement(564..869)
                     /locus_tag="PK683_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10848.1"
                     /translation="MNKNGLFNKDFAFGFVSCLCLFFAFNTFSCGRTLSRFGFGDQIL
                     KLPKDFKAMVSVGFHKDSAGTTIKDLTYETQDGQYRSVEYKDNPLSLEGSIRWEKQN"
BASE COUNT          373 a          234 c          269 g          325 t
ORIGIN      
        1 aatatgcctt ctgtacacat ctattgtata gctcatggac tgaaatgtaa aaaaagatat
       61 ccctactgga agaatcagat ttttaatcac gggaacagga gaaccgagtc cgagcagaga
      121 aagaagggaa ttggaactgg atgcaaaaaa attgtaatac ttgaagaacc ccagaatgac
      181 aatcaggttc aggaaaaggc tgagcctgag ccaggcaagg cgtgctgcag aatcctcttc
      241 tgcatcaatt ttcagtgcaa gaacatagtc tatcagtgtc gaaagaaaaa tcagaccgga
      301 aaatctccag tcccagctca tgtaaaaaat atagctcgca cccagcagaa acgagtgcag
      361 aatctgcctg gaattttccc tcccggaaaa aagcagaggg tgcaggtacc actggtatat
      421 aaatactgct gaaaaaaata taaaatattc taaactgttg aataacattt gcagtcagca
      481 ggaaatgaaa ttagatatgc gggtgcagaa aagctgaata ttctgcctcc ggattttttt
      541 tctgacggag ccttcaatca gtttcagttc tgtttttccc agcggatgct tccttcaaga
      601 ctgagaggat tgtccttgta ttccacagac ctgtactgtc cgtcctgagt ttcataagtc
      661 agatccttga ttgttgtgcc tgcagaatcc ttgtgaaatc ctacgcttac cattgctttg
      721 aagtccttag gaagtttgag tatctgatcg ccgaaaccga atctggaaag agttcttccg
      781 cagctgaatg tattgaatgc gaaaaaaagg cagaggcagc ttacgaaacc gaaagcgaag
      841 tctttattga agaggccgtt cttgttcata aaggcaaggg tgtgatgaac ggttctgcaa
      901 gtcaagtaaa tctgagagtc ttttcagtat tctgcctgaa ttgaagtgat cagttcagta
      961 atgcattctg tttttaagaa agcagttctg actgaaataa tgtgctttac ctaatttccg
     1021 ttttcaaaat tatgtctcat gagggcgagt aaacctttcg tcgctttggg aataatttga
     1081 tcagctgact cagtcttttg gaaaaaagaa aacggccgat gcgcatatga tctggcggaa
     1141 cattcattat ccggtcggaa aaaagtgcgg caagcatctg tgttccttaa gcgcacaatg
     1201 c
//
LOCUS       DAOQGJ010001639         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97120_length_1200_cov_3.108297, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001639 DAOQGJ010000000
VERSION     DAOQGJ010001639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97120_length_1200_cov_3.108297"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1200)
                     /locus_tag="PK683_20295"
     CDS             complement(<1..>1200)
                     /locus_tag="PK683_20295"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH10849.1"
                     /translation="IIAVVYLPVLALGGVEGKMFIPMALTVLYALLGAFVLSLTVVPV
                     LASYLLRGEEDPDHETWLFRKIKIYYEPLLNWFMQKREKTVFGMAGFLAFSILLFMNL
                     GGEFMPQLDEGSMLLEVARLPSVSLSESVQTSGRLEAALLKKVPEIVHAVSKTGAPDI
                     ATDPMGIDRTDTYLNIKPKEEWRFGKDRLIREIEDTVSETVPEVAISVSQPIQMRTNE
                     LIAGIRSDIGVKIFGDSLEELKTIGEKTAEILKKIEGVKDLKIEQLQGLNYIRIRPKR
                     ESLARYGVDIFDINQITESLSSGHNAGVLFEGRMKFDITVKLENTVSSLDDIRAVPVR
                     AEGQLVPLGDLASVDLESGPVQIGHENGYRRMLVEFNVRERDMMSVIQEAQEKLSSEI
                     KLPVGYR"
BASE COUNT          307 a          324 c          240 g          329 t
ORIGIN      
        1 acctgtatcc gacgggaagt ttgatttcgg aggaaagctt ttcctgggcc tcctgaatca
       61 cgctcatcat atccctttcc ctcacattga attccacaag catcctccga tacccgtttt
      121 cgtgaccgat ctgaacaggc ccggattcca gatccacaga agccagatcc cccagaggaa
      181 caagctgtcc ctccgcacgc acaggaactg cacgtatatc atccagactg cttacagtgt
      241 tttccagctt tactgtgata tcaaacttca tcctgccctc gaaaagcact cctgcattgt
      301 gcccggagga taatgattct gtaatttgat ttatatcaaa tatatccact ccgtatctgg
      361 ccagactttc tcttttcggt ctgatacgga tatagttcag tccctgaagc tgttcaatct
      421 tcagatcctt cactccttct atctttttca gaatttccgc tgtcttttca ccgattgtct
      481 taagttcttc cagactgtct ccgaatattt taacacctat atccgacctg atgcctgcaa
      541 tcagctcgtt cgttctcatc tgaataggct gtgagacaga gatggccact tcaggaactg
      601 tctctgaaac tgtgtcttca atttcacgga tcagcctgtc cttcccgaat ctccattctt
      661 ccttaggctt tatgttcaga taagtgtccg ttctgtcgat tcccatcgga tcagttgcaa
      721 tgtcaggtgc gcctgtcttg gaaacagcat gtacaatttc aggaactttt ttcagaagag
      781 cggcttccag tctgcccgaa gtctgcactg attcagaaag cgaaacagaa ggaagacgcg
      841 ccacttccag aagcatggag ccctcatcca gctgaggcat gaattccccc cccagattca
      901 tgaaaagaag tatggaaaat gcaaggaatc cggccatgcc gaaaactgtc ttttctcttt
      961 tctgcatgaa ccagtttaat aaaggttcat agtatatttt tatcttacgg aaaagccagg
     1021 tctcgtgatc cgggtcttct tcaccccgaa gcagatagga ggccagcacc ggaacaactg
     1081 tcagtgagag cacaaaagcc ccaagaagcg cgtaaagaac cgtcagtgcc atcggaatga
     1141 acattttgcc ttccactcct ccgagtgcaa gcacaggaag atagactaca gcaatgatcg
//
LOCUS       DAOQGJ010001640         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97296_length_1199_cov_3.172203, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001640 DAOQGJ010000000
VERSION     DAOQGJ010001640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97296_length_1199_cov_3.172203"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1199)
                     /locus_tag="PK683_20300"
     CDS             complement(<1..>1199)
                     /locus_tag="PK683_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNH10850.1"
                     /translation="KLDSSSFRLSQVKKLTSKLNEDINQTALDGIQSVKDATIDLEVT
                     NKQAKKLILDLQSKTDETNELLHSLKNNKDYLDTILLELKDVVKLTNEIREEARFIQD
                     GMDIIQNQKAELSNVNRDVSLIQSEVKELVHKFQDKLDVRTTNILESLAAKIVDIESL
                     LEVKAEKVDESLKAIAEGYKVQLKEEVESMVDETVGKVELANVKLDDFNSFVRDAEKS
                     LEMRLTRYKDNTDSISERIERLDLRLEEKADSVGEAVQARLSSFEKKFQERFESIFEQ
                     LSQNKEAFLSGVKMEIDLVRTEIESMSLETMTRRDEILTEARRQSETVLQNINSFFEK
                     SIDAENRLLKTAELKKGEILKEIHRFENDFRHTSESFYSDADMIKNSVMKDLQNFESD
                     LNKATSS"
BASE COUNT          279 a          283 c          214 g          423 t
ORIGIN      
        1 atggaggaag ttgccttgtt cagatcgctt tcaaaattct gcaggtcttt cattacagaa
       61 ttcttgatca tatctgcatc tgaataaaat gattcacttg tatgtctgaa atcattttca
      121 aatctgtgaa tttccttaag gatctctcct tttttgagtt cagctgtttt cagaagacgg
      181 ttttctgcat cgattgattt ctcaaagaag gaattgatgt tctgcagtac agtttcagac
      241 tgccgtctgg cttcagtcag aatctcgtct cttcgagtca ttgtttcgag actcatactt
      301 tctatttcag tccgcacaag atcaatctcc attttgactc cggaaagaaa tgcttccttg
      361 ttctgagaga gctgttcaaa tatggattca aatctttcct gaaatttttt ctcaaatgaa
      421 gaaagtctgg cctgaacagc ttcacctaca ctgtccgctt tctcttccag tctgagatca
      481 agcctctcta ttctttctga gatggaatct gtattgtcct tgtatcttgt aagtctcatc
      541 tctaaagact tctcagcatc cctcacaaag ctgttgaagt catcgagttt gacgttggcc
      601 agttccactt tccctaccgt ctcatccacc atcgactcga cttcttcctt gagctggacc
      661 ttgtatcctt ctgcaattgc cttcagagac tcatccactt tctccgcctt cacttccaga
      721 agactttcga tatctacgat ttttgcagca agggattcca gtatatttgt cgtgcgtaca
      781 tccagcttgt cctgaaattt gtgcacaagc tcttttactt cgctctgaat cagactcaca
      841 tccctgttta cattggaaag ctctgccttc tgattctgta tgatgtccat cccgtcctga
      901 atgaatcttg cttcctcacg gatctcattt gtgagtttga caacatcctt cagttcaaga
      961 aggatagtat caagatagtc cttattgttt ttcagcgaat gcagaagttc atttgtctcg
     1021 tcggttttcg actgaagatc cagaatcagt tttttggcct gtttattggt aacttccaga
     1081 tcaatagtgg catcttttac tgactgaatt ccgtccaaag cagtctgatt gatgtcctcg
     1141 ttcagcttgg atgtcagttt ttttacctga ctcagacgaa aactcgaact gtccagctt
//
LOCUS       DAOQGJ010001641         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97437_length_1198_cov_2.899388, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001641 DAOQGJ010000000
VERSION     DAOQGJ010001641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97437_length_1198_cov_2.899388"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..567)
                     /locus_tag="PK683_20305"
     CDS             complement(46..567)
                     /locus_tag="PK683_20305"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH10851.1"
                     /translation="MEAMNMTIKNLIPWNRKDKRELTESNLDSPFLSLHREMNRLFDN
                     FFNDFPSVSTGRYSEVSSFSPRINIAENEKEIRIEAELPGIDEKDLSVEIKGRTLSIH
                     GERKAKKEEKKENYHIVESSYGSFYRSVELPEEADLDKVEAKSKDGITTIIIPKLENS
                     KTAKKVIQVKRSE"
     gene            912..1142
                     /locus_tag="PK683_20310"
     CDS             912..1142
                     /locus_tag="PK683_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10852.1"
                     /translation="MKTGMGWCVQRSECGKAGLNCIGAVKAFSPNQNSSQDQLLAFCA
                     TRETSCRDWCHKHCYYAKGSWCYTEKYHLIPK"
BASE COUNT          340 a          249 c          221 g          388 t
ORIGIN      
        1 gaaaagaagc ggggcattcg aagaacaccc cgcgagcgac aggaattatt cagaacgttt
       61 tacttgaatc accttcttgg ctgttttaga attttctaat ttaggaatga tgattgtagt
      121 gatgccgtct ttggattttg cttccacctt atccaagtca gcttcttcgg gaagttctac
      181 cgatctgtaa aaactgccgt acgaactttc cacaatatga tagttttctt ttttttcttc
      241 cttttttgcc tttctctccc cgtgaatgga aagagttctt cctttgattt ccacagaaag
      301 atccttctca tcaattccgg ggagttctgc ttcaatgcgg atttcttttt cattctccgc
      361 aatatttatt cttgggctga aggaagagac ttcactgtat ctcccagtgc ttacactggg
      421 gaaatcgttg aaaaaattgt caaacagtct gttcatttcc cgatgaaggg aaagaaacgg
      481 agaatctaaa ttagattcag tcagctctct tttgtctttt ctattccaag gaatcagatt
      541 tttgattgtc atattcattg cctccatttt tagcactcta accattagag tgctaaaaca
      601 ggaaaaagtt cgtgactttt taaaattttt tttagattcg aagtccttcg tttccgatca
      661 gaatgcgcaa aacctgaaaa ttacagtcca cgcagtgaaa attgaaaata cttgtaaaat
      721 aaaccttaca catccatatc attttccgca ggagtacatc aatttttcag attgacccgg
      781 aatttcagaa tactcttctg tatgatatga gaaatttatt gaaaattcct gcagatcaat
      841 catacattca tcattttttt tcattactgt ttttttcatt attcattttg gccgcagagg
      901 gatgcggaga gatgaagacc ggaatgggat ggtgcgttca gcgcagtgaa tgcggaaagg
      961 caggattaaa ctgcataggt gcagtcaaag cattcagtcc aaatcagaac agctcacagg
     1021 atcagctcct tgctttctgt gccacaagag aaacctcctg cagagactgg tgccataaac
     1081 actgctacta tgccaagggg tcctggtgct acactgaaaa ataccatctg attcctaaat
     1141 aattccgact caataaattc cagcctgatt gaaatgcttc gaatactttt tatcactc
//
LOCUS       DAOQGJ010001642         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97501_length_1198_cov_1.980752, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001642 DAOQGJ010000000
VERSION     DAOQGJ010001642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97501_length_1198_cov_1.980752"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..690
                     /locus_tag="PK683_20315"
     CDS             112..690
                     /locus_tag="PK683_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10853.1"
                     /translation="MSAPSDSISFLTTGSVGSSFSGFPAADSKNTSGFNGSTLTYTSG
                     FDWLSFDTTFKGIGLYNSAAFPSTLHRGQCSGTNPCAIWDFSLKSTDTVLRNANITGG
                     GTGCPTSSSVFTHTYSTGSVTVLRNAVEIIGDGVGNENGICESNEDCLLTPNHGAYQG
                     HGNLVKSSTLSGGCADVTSLGIKLYQYETNGY"
BASE COUNT          360 a          238 c          267 g          333 t
ORIGIN      
        1 gtttcaggtt ctggtaactt ttatggcaga gggatgatta gaggttcagg aaacaccacc
       61 aattgcccat acgcaacttc ttttactgga attacttctg gaacttgcgc tatgtctgct
      121 ccttctgatt caatcagttt tcttactacg ggcagtgtcg gcagttcttt ttccggattt
      181 ccggctgccg actccaaaaa tacatcaggt ttcaacggaa gtacattgac gtacacttca
      241 ggatttgact ggctgagttt tgatactact ttcaaaggaa tcggtcttta caacagtgca
      301 gcttttccga gcacattgca cagaggacag tgttcaggaa caaacccgtg tgctatatgg
      361 gacttttcat taaaatctac tgatactgta ctgcgaaatg caaatattac cggaggtgga
      421 accggctgtc cgacttcctc ctcagtattt actcatacgt acagcacagg ttctgtgact
      481 gtacttcgaa atgcagttga gattattgga gacggagtcg gaaatgagaa cgggatctgt
      541 gagtccaatg aagactgcct cctgacccca aatcatggtg cctatcaggg acacgggaat
      601 cttgtgaagt cgtccactct gtcaggggga tgtgctgacg tgacttcact cgggataaag
      661 ctgtatcagt atgaaacaaa cgggtactga gcagtccaaa aaatgagtaa tttccggata
      721 aatctgccat ttacattacg caaatacgat atgctgttta gctgctgtaa ttttcagaag
      781 gcattagtaa aatctaatga accagtaaaa acagcagctt cgctgcttgc gggattttta
      841 gcagctttac agtttcccca ttttttggac agtaaaattg aaaaaacaag gcggtaaata
      901 aattgcaaaa cccgcaaaaa agagaagcag tgagacagcg gagaatcaga atacaatata
      961 atgattatga atctgattga ccgcgaatac accagacatc cgttttccga aagaaaaggg
     1021 ttatgactta aaccgcaagc gtattgagag actgatgcag tgatggaact tttgctcagt
     1081 ataatcttaa aaagtgcaat ttgaacggaa aatatcagat ggtctttgat gaaggttatt
     1141 tttctgacaa ctgtgtaaca accggcacaa taaaagtcga ccgggaattt gcttatct
//
LOCUS       DAOQGJ010001643         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97561_length_1197_cov_3.389667, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001643 DAOQGJ010000000
VERSION     DAOQGJ010001643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97561_length_1197_cov_3.389667"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..664
                     /locus_tag="PK683_20320"
     CDS             155..664
                     /locus_tag="PK683_20320"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH10854.1"
                     /translation="MVKNSFIDNEYDDEEEDTLQDMYLIFCCADRHYSVEVRYVSEIV
                     ALQTVTEIPDMPSYFKGVINLRGKILPVLDMRLRLGYSEMQYHERTCCIVSDKFGMIV
                     DSVTEVARIPASEITGAPNIGEGVSHQYVSGVSRSAEELRIILNIKSLLDPSEGQDEL
                     MSALNKTVQ"
     gene            667..837
                     /locus_tag="PK683_20325"
     CDS             667..837
                     /locus_tag="PK683_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10855.1"
                     /translation="MDSSFKEFLFSSYEDLKHMEDCIVSIEEILNSEGLPDPELVNVL
                     FRHMYGIKHSAD"
     gene            842..1072
                     /locus_tag="PK683_20330"
     CDS             842..1072
                     /locus_tag="PK683_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10856.1"
                     /translation="MIQNNAFSSEETASASMELSEQAGGIDRMVKRFKISELPVHIIK
                     TERNESRNTVLHRSESGRVLLKKKTPSEVISL"
BASE COUNT          378 a          203 c          268 g          348 t
ORIGIN      
        1 aagacaggag gcagacagaa atctccaaac agaaatcatt ccggcagcag tcctgttaaa
       61 aaaaactcac atatcatatc gctaggagaa gctctatagt tgatgaatga gacgaagtgc
      121 tgcataaaag gaataaagtt taactgaggg gcaaatggta aaaaacagct tcattgataa
      181 tgaatacgat gatgaggaag aggatactct gcaggatatg tatctgattt tctgctgtgc
      241 cgacaggcat tattcagtag aagtccgtta tgtttctgaa attgttgctc ttcagacggt
      301 cactgaaatt cctgatatgc cttcttattt caagggagtg atcaatcttc gcggtaagat
      361 ccttcctgta ttggatatga gactccgttt aggatattct gaaatgcaat atcatgaaag
      421 aacatgctgt attgtttctg acaagttcgg tatgattgtg gattctgtga ctgaggttgc
      481 aagaatccct gcttcagaaa ttacgggagc accgaatata ggagagggcg tttctcatca
      541 gtatgtcagc ggtgtttcca ggtctgcgga agagctcaga attatactga atataaaatc
      601 tcttttggat ccgtccgaag ggcaggacga gcttatgtct gctttgaaca agacagtaca
      661 gtaactgtgg attcatcttt taaagagttc ctgttttcct cttacgaaga tctgaagcat
      721 atggaagact gtattgtctc cattgaagaa attctcaact ctgagggtct gcctgatcct
      781 gaattggtga atgttctttt caggcatatg tacggtataa agcattcagc agattgagca
      841 ggtgatacag aataatgcat tcagttcaga agagactgca tctgcaagta tggagctttc
      901 agaacaggca ggaggaatcg acaggatggt gaaacgtttc aaaatttccg aacttcctgt
      961 tcatattatc aaaacggaaa ggaatgaatc aagaaacact gttctgcatc ggtcagaatc
     1021 cggaagagtt ttactgaaga agaaaacacc ttctgaggtt atatcactgt gagcaaataa
     1081 atggaaaact gatttgatta ataagagaaa tcccctagct gtgttgacta tatttcttaa
     1141 aaaagaactt tttttagttt gcaaactgaa aattcatctg gaaatcattt ttacagt
//
LOCUS       DAOQGJ010001644         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97574_length_1197_cov_3.045534, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001644 DAOQGJ010000000
VERSION     DAOQGJ010001644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97574_length_1197_cov_3.045534"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PK683_20335"
     CDS             complement(<1..837)
                     /locus_tag="PK683_20335"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNH10857.1"
                     /translation="MSEVKISEDLKYLLDCLQEVFLELQENRLVEFVQFLQGKAEPPK
                     NLELDKTVQLLSLYFQLLNMVEENSAVQFRRRAETEKGFTAISGLWGEKLKDLTDKGF
                     TAEQIAGIFPEIHIEPVLTAHPTEAKRATVLEQHRELYLLLVKKENRIWTPFERDSIK
                     EEIKTALERLWRTGEIYLERPDISSERRNISHYLRNVFPNVLPSADLRLRGAWKELGF
                     SSDYLDSYKKFPKISFGNWVGGDRDGHPFVTPEITEETLEEFRKYSLELAEKEILRLG
                     GHL"
BASE COUNT          325 a          279 c          227 g          366 t
ORIGIN      
        1 taaatgcccg cccagcctaa gtatctcttt ttcagccagt tccagggaat atttcctgaa
       61 ctcttcgagt gtttcttcag tgatttccgg cgtaacgaaa ggatgtccgt ccctgtcgcc
      121 tcccacccag tttccaaaac ttatcttggg aaatttttta taagaatcca gataatcaga
      181 tgaaaaacca agttctttcc atgctcctcg aagtcggaga tctgcagagg gaagaacatt
      241 agggaataca ttccgcaggt aatgggatat attccttctt tcagaagaaa tgtccggcct
      301 ttccagataa atctctcctg ttctccagag cctttccagt gcagtcttga tttcttcctt
      361 gatggaatct ctttcaaaag gagtccagat tctgttttct ttttttacga gaagaagata
      421 cagctcacgg tgctgctcaa gcaccgttgc acgttttgct tccgtaggat gggcggtcag
      481 aactggttca atatgaatct caggaaaaat gccggcaatc tgttccgcag taaatccttt
      541 atcagtcaga tccttcagtt tttctcccca aagcccggag atggctgtga aacctttttc
      601 tgtttctgct cttcttctga actggactgc tgagttttct tccaccatat tcaggagctg
      661 aaagtaaagg gagagaagct gcactgtctt gtccagttcc aaattcttcg gaggctctgc
      721 cttgccctga agaaactgaa caaattccac cagtctgttt tcctgaagtt ccagaaatac
      781 ttcctgcagg cagtccaata gatactttaa atcttccgaa atttttactt cactcatttt
      841 tgcactccgt agtaatgata aaatatcttt taatcagcat aagatcacgg gatactcgac
      901 cagctgactt atttaaaaaa aagcaaaacg cctctcatca actaagaatg agactcagtt
      961 tttctgtcac ttttcaggag agcggaaaat tattcgaaca ttttatatac ttggcagctt
     1021 ttttaaatgc tcacaaaaaa aacctgagac actggatttc cagagtttac aaccctcggc
     1081 ttcgagtttc ggcttccgag cactccgatt tgaacggaag aggagggtgt ccaccatcat
     1141 ccggggcaat cataacagta tatattataa catgtgcacc aatttttctc taatgat
//
LOCUS       DAOQGJ010001645         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97716_length_1196_cov_3.128834, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001645 DAOQGJ010000000
VERSION     DAOQGJ010001645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97716_length_1196_cov_3.128834"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PK683_20340"
     CDS             <1..404
                     /locus_tag="PK683_20340"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH10858.1"
                     /translation="NVYLVDYYGWTPLFYAVDRGNAEITRLLLEKNSDVNTKDQDRTT
                     PLMKAAVKGFTEIAALLVKHRPDLNAKDKDGETALIHASAKGHLEIVKLLVENNADIN
                     AENKNHNTALIYANYKKFTEIAEYLKSKGSK"
     gene            427..1038
                     /locus_tag="PK683_20345"
     CDS             427..1038
                     /locus_tag="PK683_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004770583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNH10859.1"
                     /translation="MTKILSLLSAFILFANCGGYNKIQENDEKVNSAWAEVLNQYKRR
                     TDLIPALVKTVQGAADFEKSVLTDVTRARSNVGGIQVTPEILKNPDELQKFMTAQTQL
                     SGALQRLLVVAEKYPDLKSNENFLSLQSQIEGTENRLAVSRTRYIKAVEEYNLSIRQI
                     PGNIYASIFGYKPKASFTIENADAIKKMDESVAEPPKVDFGKK"
BASE COUNT          404 a          257 c          251 g          284 t
ORIGIN      
        1 caaatgtcta cttggtggac tattacggct ggactccact tttttatgct gtggacaggg
       61 gaaatgcaga aataacacgt ctccttctgg aaaaaaactc tgacgtaaac acaaaggatc
      121 aggacagaac cactcctctg atgaaggctg cagtgaaagg attcactgag attgctgcac
      181 tgcttgtaaa acacaggcct gacctgaatg caaaggacaa ggacggagaa acggctctca
      241 ttcatgcgtc agccaaaggg catctggaaa ttgtaaaact tctggtggaa aacaatgcgg
      301 acatcaatgc agagaacaaa aatcacaaca ctgcactcat ttatgccaac tataaaaaat
      361 ttacggaaat cgctgagtac ctgaaatcca aagggtctaa atgacaataa aattcaagga
      421 tggtttatga caaaaatctt atcattgctc agtgcattca tactgttcgc aaactgcggc
      481 ggttacaaca aaattcagga aaatgatgaa aaggtaaatt ctgcatgggc agaagttctg
      541 aatcagtaca aaagaagaac agacctgatt cctgctctgg taaaaaccgt acaaggtgca
      601 gctgatttcg aaaaatccgt tctgactgat gtaacccgtg caagaagcaa tgtaggcggg
      661 attcaggtca ctcctgaaat tctgaaaaac ccggatgaac ttcagaaatt tatgacagca
      721 cagactcagc tctccggcgc actgcagagg cttctggttg tggctgaaaa atatcctgat
      781 ctgaaatcaa acgaaaactt tctgagcctt cagtcccaga ttgaaggaac agaaaacaga
      841 ctcgcagtat ccagaacaag atatatcaag gccgtcgaag agtacaatct gagcataaga
      901 cagattccag gaaatatcta cgcctccatc ttcggataca aaccaaaagc atcctttaca
      961 attgaaaatg ctgacgccat caaaaaaatg gatgagtcag tcgctgaacc tcctaaggta
     1021 gatttcggca agaagtaaga attttaaatc caatgaagcg aggtgaaaac ctcgcatttt
     1081 tgaagcctgg ttttcatagg ctgttgcaca cctgatctcg cttccatctg cacctgatgc
     1141 tgttttttga aaccagatca cagatgaaca cgtctccgtt tttcagaatg acttct
//
LOCUS       DAOQGJ010001646         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_97758_length_1196_cov_2.368975, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001646 DAOQGJ010000000
VERSION     DAOQGJ010001646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97758_length_1196_cov_2.368975"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..>1196
                     /locus_tag="PK683_20350"
     CDS             328..>1196
                     /locus_tag="PK683_20350"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH10860.1"
                     /translation="MNKGFAVLILLLFQFCSMSISRKTAELLPKADLLFRILNISKSG
                     FTVSGTVSGLTSEGLILSSGTSPNVTVSVASGSASFQFAGTFADGTVYKITASSVPST
                     QSCTVQNGSGTVSGANITNVSVTCYPNFPSVVSITPADGASNLNFSTFITLNFSGPMD
                     ISSLTVNTVDNNCNSTIQVSEDDFSSCIPLDSAVSSTGNSSVSFKAKFGYTYYKTVKV
                     RVLSSAKNILGNNMQSAYTSSGFSGFSIAGLRIHFQSHSVYQSNSTNLLSWTDLSGNG
                     NTVSQATGANQPL"
BASE COUNT          342 a          261 c          240 g          353 t
ORIGIN      
        1 ctgtatccga tttatggagg atttttagga tacaatgtca gactggattt tgccttggaa
       61 aatgatgaat agccttggtt ccgcatacac attccgagac tgagtccagg tatatgcacc
      121 ggcggataat gatcggaatg aaaagctttc tcaaagtgca gttcaaatat ctcaatttta
      181 gaaaaaagct tccggtgaaa ccgtaaaagg tcttgatgtc agaaatgtta tattagaatt
      241 ttatgcagac catgtacaaa aaaaacgctc tcagggaaaa aataagttca cggctgttat
      301 aagaatgtgt gcaaacggag tcgtattatg aataagggtt ttgctgtttt aattctgctt
      361 ctgtttcaat tctgcagtat gtccatcagc agaaaaactg ctgaacttct gcccaaagct
      421 gatctgcttt ttcgaatttt gaatatatct aaatccggat tcacagtcag cggaactgta
      481 tcaggactga cctcagaggg actgattctt tccagcggaa catcaccgaa cgtaacggtc
      541 tctgtcgcat ccggttccgc ctctttccag tttgccggaa cctttgcaga cggtacagtg
      601 tacaaaatca ctgcttcttc tgttccttcc actcagtcct gcacagttca aaacggatca
      661 ggaacggtat ccggagcaaa tatcacaaat gtttccgtga cctgttatcc gaactttcct
      721 tctgttgttt caataacacc cgctgacgga gcgtcaaatc tgaatttcag caccttcatt
      781 actctgaatt tttccggccc catggatata agttcactta ctgtaaatac tgtggataat
      841 aactgcaatt ccacaatcca ggtttccgag gatgatttca gttcctgcat ccctctggat
      901 tctgcagtga gctccacagg aaattcttca gtaagtttca aggcaaagtt cggatacact
      961 tattataaaa ctgtgaaagt cagagttctt agttcagcga aaaatatact agggaacaat
     1021 atgcagtcag catacacatc ttccgggttt tcaggattca gtatcgccgg gctgagaatt
     1081 catttccagt ctcattctgt ttatcagtca aacagtacaa atctgctttc ctggacagat
     1141 cttagcggaa acggaaacac tgtttctcag gcaacaggag caaatcagcc gctttt
//
LOCUS       DAOQGJ010001647         1193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98146_length_1193_cov_3.608084, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001647 DAOQGJ010000000
VERSION     DAOQGJ010001647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98146_length_1193_cov_3.608084"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..321
                     /locus_tag="PK683_20355"
                     /pseudo
     CDS             73..321
                     /locus_tag="PK683_20355"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
     gene            complement(379..966)
                     /gene="leuD"
                     /locus_tag="PK683_20360"
     CDS             complement(379..966)
                     /gene="leuD"
                     /locus_tag="PK683_20360"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNH10861.1"
                     /translation="MKSWKKHIGKAVPLCRDDIDTDQILPAVFMRKISRTGFGEHLFH
                     NWRYLDSAGETLNPAFILNQPRYSEATVLIAGRNFGSGSSREHAPWALYQYGFRVIIS
                     ESFADIFYNNCFKNGIALIRLSEKDILEILEFEKRAGAGLTVTADLEKSFLECAGKKY
                     PFEISDTLKERILFGMDDIDRTLKNEKLIDSYLAG"
     gene            complement(968..1099)
                     /locus_tag="PK683_20365"
                     /pseudo
     CDS             complement(968..1099)
                     /locus_tag="PK683_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
BASE COUNT          315 a          302 c          229 g          347 t
ORIGIN      
        1 atatatgctc tgaatctttt taatattgca gacagagaag agtatcttgc ctaatcacgg
       61 cgttcggcaa aggcagtagc cgagcggggc ggcagagttg tcgcactggg aaaattcaga
      121 gaggcgctga aaggagatat tgctccccga aaagtgctga ttgttgtcga atgggaatca
      181 gtcgaagcat tcaacagtta cgtaaacgat ccggcacttg cagacctcca ccctcacaga
      241 gaaaaaggcg gaggggacta tgtctggcac ctgtttgacc gaatcgatga tctgagacct
      301 atactgtatc caaacggata atatctgtat taaaatctcc gctcagcaat aaattaccgc
      361 aggctgagcg tctgctttct atcccgcaag atatgaatca atcagttttt cattcttcag
      421 ggtcctgtca atatcatcca tcccaaaaag aattctttcc ttcagtgtat cactgatttc
      481 aaaaggatat ttttttcctg cacattccag aaaagatttt tcaagatctg cagtcacggt
      541 caatcccgct cctgctcttt tctcaaattc cagaatttcc agaatatctt tttcagagag
      601 ccgaatcagt gcaattccat tcttgaagca gttgttatag aatatatccg caaatgattc
      661 agaaataatt accctgaatc cgtactgata cagagcccat ggagcatgtt ccctgcttga
      721 gccggaaccg aaatttcttc ctgcaatcag aacggttgct tccgaatacc tgggctgatt
      781 cagaataaat gcaggattca aagtctcccc cgcagaatcc agatatctcc aattatgaaa
      841 aaggtgctcc ccaaaccctg ttctcgatat cttcctcata aatacggccg ggagaatctg
      901 atccgtgtca atatcatccc tgcagagagg aacagccttt cctatatgtt ttttccatga
      961 tttcatatta cactccattc tttgctaaat cgttcaaatc acccaagtgt ccgagtattc
     1021 ctgccgctgc agccagttcc gggctgagta tatgcacgcg tgcccccttt ccctgcctcc
     1081 cttcaaaatt tctgttcgca agttttccgg tttctcactt ccttctccct ttcgtttgtc
     1141 tatgaaactg aatcgctcct ctcctctccc tactctcgag ttgtgtgata atg
//
LOCUS       DAOQGJ010001648         1193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98161_length_1193_cov_3.202988, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001648 DAOQGJ010000000
VERSION     DAOQGJ010001648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98161_length_1193_cov_3.202988"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            442..>1193
                     /locus_tag="PK683_20370"
     CDS             442..>1193
                     /locus_tag="PK683_20370"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNH10862.1"
                     /translation="MKAKKICIFIILISFFHCLKAGRKGFQFPAVGTITLPSPSTATS
                     TAANTAASTSASTGTSGPTYPSPVIKTGQTGCWDGAGASVSCTGTGIDGEFQYGRARS
                     FTDNGDGTVTENGTGLYWQKCSAGLSGATCATGTAATDTWANAQTYCSGLSLAGRTWR
                     MPTYNELANLANYSVSAPAADSAFFPNTVSGNYWVNNTYTASPTFAYYFTFLAGASST
                     LAKTSTLNARCVSGTSVTSSYTDNGDGTVRDQA"
BASE COUNT          337 a          287 c          280 g          289 t
ORIGIN      
        1 ttttcttatg gattcgactc agctcaccac aggtcgaggt gggcggtgaa aatcatggtt
       61 cggtaaactc accacaccgc tcggcaaccg tctctcctga atatagaacc ttaacttgaa
      121 aacatagcct accgggagag tttcccactt gcagcagctt cagagattgc agcagcttca
      181 gagattgcgt tggtttaaag gcgctattgc taacaaatga tctcagcaaa aaatgcagtt
      241 ctgtttatta actcttaatt cattcaaaca caaagcccag tccgccgccc tccaaaaaat
      301 cctagacagt ttaaaattat cgattgttct ggaaactgtc caaagacaga atccgttcaa
      361 aatcggctgt gtaaaatgcg gatgcgcgta gtcatcattc gccgaggact ctttctttga
      421 aaatgaaaag aggtgcatca gatgaaagcg aagaaaatat gcatttttat tatattaatt
      481 tcattttttc attgtctgaa agcaggccgg aaaggatttc agtttcctgc ggtaggtaca
      541 attactctgc cgagtccgag cactgcgact tccactgccg caaatacagc agcatcaact
      601 tccgcctcaa ccggaacatc cggaccgacc tatccttccc cggtaatcaa aacaggacag
      661 accggatgct gggacggagc cggagcctcg gtgtcatgta cggggacggg aattgacgga
      721 gaatttcagt atgggagagc gagaagcttt accgataacg gagacggaac agtgacagaa
      781 aacggcaccg gtctttactg gcagaaatgc agtgcagggc tttccggcgc gacttgtgcg
      841 actggaacag cagcgactga tacttgggca aatgctcaga catactgttc tggactgagt
      901 cttgctggaa gaacctggag aatgcccacg tacaatgaac tggcaaatct ggcaaattat
      961 tcagtgagcg ctcccgctgc cgacagtgct ttttttccaa atacagtcag cggtaattac
     1021 tgggtgaata acacatatac agccagtcct acgttcgctt attatttcac cttcctagcc
     1081 ggtgcatcga gcacactcgc taaaacaagc acactgaatg cccgctgtgt ttcgggaaca
     1141 agtgtgactt cttcctatac ggacaatgga gacggaactg tcagagatca ggc
//
LOCUS       DAOQGJ010001649         1192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98283_length_1192_cov_4.054529, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001649 DAOQGJ010000000
VERSION     DAOQGJ010001649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98283_length_1192_cov_4.054529"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PK683_20375"
     CDS             complement(<1..654)
                     /locus_tag="PK683_20375"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNH10863.1"
                     /translation="MNRLFPLLLFFSLFLLDKVFLIPPVKEKFVKNRLSVYEGLFTFQ
                     DKVYKEHLNHWRDIFGSDQVTVKKQMVFFGSSRSAEFADLKDADFLNNPYWNQVNPIP
                     PVTSMTVRAGNFTHVYLLYRNFIKNYGTESVLVLELNSVSFSMGSVFRDKKETQEIPP
                     NMFRDLFPSLSNKERNEYLSSQFFVLNRLNLNFTGKENQDVTKVMDSLLILKKALEKK
                     "
     gene            complement(656..>1192)
                     /locus_tag="PK683_20380"
     CDS             complement(656..>1192)
                     /locus_tag="PK683_20380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNH10864.1"
                     /translation="LRDYLYFPMGGSRVSSVRASVNVMLTMTLAGLWHGANWNFVLWG
                     FVIGLSLVIETNILDRFYFWRESKTGTAVTVRWFLVFCFWTFIAVIFRVNQVSDLAVL
                     AKGLFAVKPAMGKIRPDQFASLCLLTVLLHYAELKIEILNRKIFHSKIFFAAAAAVLM
                     FVLADLSNKQVQFIYFQF"
BASE COUNT          388 a          262 c          225 g          317 t
ORIGIN      
        1 ctttttctcc agtgcctttt tgagaatcag gagagaatcc atcaccttcg taacatcctg
       61 attttccttt cctgtaaaat tcagattcag tctgttcaga acaaaaaact gagagcttaa
      121 gtactcattt ctttccttgt tggaaagact tggaaaaaga tccctgaaca tattgggagg
      181 gatttcctga gtttcttttt tgtcacggaa aacagaaccc atactgaatg aaactgaatt
      241 cagttccaaa accaggacag attcggttcc gtaattctta atgaaatttc tatataagag
      301 atatacatga gtgaaatttc ctgctctgac tgtcatcgat gtcacgggag gaattggatt
      361 gacctgattc cagtatggat tgttcagaaa atctgcatcc ttcagatcag caaattccgc
      421 acttctggaa gaaccgaaga ataccatctg cttttttacg gtcacctgat cggaaccgaa
      481 aatatctctc cagtgattca gatgttcttt gtacaccttg tcctgaaaag taaaaagtcc
      541 ttcatataca gaaagtctgt tctttacgaa tttttccttg acaggcggaa tcagaaatac
      601 cttgtccaga aggaaaagag agaaaaaaag cagtaatgga aataatctat tcatatcaga
      661 actggaaata aataaactga acctgcttat tggacagatc cgctaaaaca aacatcagaa
      721 cggcggcggc ggcggcaaag aaaatctttg agtgaaatat ctttctgttc agaatttcaa
      781 tcttcagctc ggcataatga agcaggactg taagcagaca gagagaagca aactgatcag
      841 gccgtatttt tcccatcgca ggttttactg caaaaagtcc ttttgcaagt acagccagat
      901 ccgaaacttg attgaccctg aatatgactg caatgaaagt ccagaagcag aataccaaaa
      961 accatctgac agtcactgct gttccagtct tgctttccct ccagaaataa aacctgtcca
     1021 ggatattcgt ctcaatcacg agagaaagac caatcacaaa tccccagaga acaaagttcc
     1081 agttagcacc atgccagagt ccggccaacg tcatagtcag cataacgttc actgaggctc
     1141 gaacactgga aaccctgctt cctcccattg gaaaatacag ataatcccga ag
//
LOCUS       DAOQGJ010001650         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98444_length_1191_cov_3.664613, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001650 DAOQGJ010000000
VERSION     DAOQGJ010001650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98444_length_1191_cov_3.664613"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PK683_20385"
     CDS             complement(<1..921)
                     /locus_tag="PK683_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10865.1"
                     /translation="MTDSSGNFFGLKKISYTLFDLVFRWAGRFNGYVFAVSFFSTGLS
                     GAASTLFALYYFDAIDVAVNPVLMNILKVSIAVYWLLIMTQLGFWHFLGFSGIGTHVT
                     VLNRSILTWPNPRFRDDLKENDYIAVYKALMSFPLFNGINLFVWVFVLVNLMIGVSVL
                     VERSFNWELIRAILVVGVIALFICLCFSYVLSEVITGEMREKCKRKMSELGIPFEDKS
                     LFTIRIKLFFFLALFVVNLFLSNTLTYYNSKEIEKVIHFSIIAVIISLLMAHAIFNLI
                     YHALKQIENAAYDLMQGGTGQVFVRSLDREF"
BASE COUNT          387 a          260 c          228 g          316 t
ORIGIN      
        1 gaattccctg tccagggatc ttacaaatac ctgcccggtt ccgccctgca tcaggtcata
       61 ggctgcattc tctatctgct tcagtgcatg atagatcaga ttgaaaatcg catgggccat
      121 aaggagtgat ataatcactg cgattattga aaaatggatt actttctcaa tttccttgct
      181 gttgtaatag gtaagggtat tggaaagaaa aagattcacg acaaaaagag cgagaaagaa
      241 aaagagtttt attctgatgg tgaacagtga cttgtcttca aaaggaatcc ccagttcgga
      301 catcttcctt ttgcattttt ctctcatctc gcccgttatg acttctgaaa gaacataact
      361 gaagcagaga cagatgaaga gcgcaatgac tcctacgaca agaatagccc gtatcagttc
      421 ccagttgaaa cttctttcca ccagaaccga gacccctatc atcagattta caagcacaaa
      481 gacccataca aaaagattga ttccgttgaa cagaggaaat gacatcagtg ctttatatac
      541 agctatataa tcattttctt taagatcatc cctgaaacgc ggatttggcc atgtaagtat
      601 gctcctgttc agaacggtca catgagttcc tattccggaa aaaccgagaa aatgccagaa
      661 accaagctga gtcataatca gaagccagta gacagcgatg ctgactttaa gaatattcat
      721 cagcactgga ttgactgcaa catcgatcgc atcaaaatag tacagagcaa aaagagtgga
      781 tgctgctcct gagaggccgg tggaaaaaaa ggaaacagca aacacgtatc cgttgaatcg
      841 gcctgcccat ctgaacacca gatcaaaaag agtgtatgaa atttttttta gaccaaagaa
      901 attgccggat gaatcagtca tacattacct gaataaaaaa tcctgtcatt aaaaacctgc
      961 cgcacacagt catcctcata aaacggcact gttttgagct attcaaaatt cggtctctca
     1021 aaaaaaagta aattcatgga attgattctc attattcaca gtctgctgcg tcatgcagcc
     1081 cttctccttc ttgctgctct tgtttcagtt tcactttcca gatggattgg aaagaaagaa
     1141 ttttccggac tggaaaagaa actctccctg ttcacaatga tttcattcga c
//
LOCUS       DAOQGJ010001651         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98463_length_1191_cov_3.192782, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001651 DAOQGJ010000000
VERSION     DAOQGJ010001651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98463_length_1191_cov_3.192782"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..612
                     /locus_tag="PK683_20390"
     CDS             145..612
                     /locus_tag="PK683_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10866.1"
                     /translation="MRIFDCEIRIDENSRWFFRGNEIIHENVLRHFKENLGEDEKGIF
                     ILNQYGDLTEKGYLYSEGFPLRLVSISGKSFFDETGKEYSADGFIFYENSRFGVFAST
                     ERKKIFFSLSRDFCGAMSSFMEEEGGEVFLKLGDSKIRIQEFEGKITVNVPDL"
     gene            complement(613..909)
                     /locus_tag="PK683_20395"
     CDS             complement(613..909)
                     /locus_tag="PK683_20395"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH10867.1"
                     /translation="MNIEVTVDSDLEDLIPDYLENMRQIPNLLNNALAGSDFETIKRE
                     GHKMKGHGSAYGFHKITEIGLEVQKAGNEEDIEHIKKMISALEDYLKKVKIVYK"
BASE COUNT          340 a          229 c          256 g          366 t
ORIGIN      
        1 tcttcctccc gaaaatatgg aaatccctcc ctggatcatt cagtctaaaa accaggccgg
       61 gatgacaatg agctatactg agctgaacaa agaaatgaag agactgaagg gttatgcata
      121 cagatacaga gactgacttg gcctatgcga atatttgact gcgaaatcag aatagatgaa
      181 aacagccgtt ggttctttcg cggcaatgaa atcattcacg aaaatgtact ccgtcatttc
      241 aaggagaacc tgggtgagga cgaaaaagga atatttatcc tgaatcagta cggtgatctg
      301 actgaaaagg gttatcttta ttcggaggga tttcctctca gactcgtgag tatttccggg
      361 aagtcttttt ttgatgagac aggcaaggag tattctgcag atggatttat tttttatgag
      421 aacagcagat tcggagtttt tgcatccaca gaaaggaaga aaattttttt cagtctgagc
      481 agagacttct gcggtgcaat gtcttcattt atggaagagg aaggggggga ggtttttctg
      541 aaactgggtg attctaaaat caggattcag gaatttgaag ggaaaatcac agtaaatgtg
      601 ccggatcttt agtcatttat aaactatctt tacttttttc agatagtctt ccaaggctga
      661 tatcattttt tttatatgct caatatcttc ctcatttcct gccttttgta cctccagtcc
      721 aatttcagtg attttgtgaa atccgtatgc tgaaccgtga cctttcatct tgtgtccttc
      781 tcttttgatc gtttcaaaat cggaaccggc gagagcgtta ttgagcagat taggaatctg
      841 acgcatattc tccagataat caggaatcag atcttccaga tctgaatcta cagtcacttc
      901 aatattcata taccgccctt cgaaaaatcc ggtttcactg ccgagtcctg ctcaatttta
      961 ctagtaaggt tagcattgat ttttccgata ctataagcat gaggctcgtg ttaatttcaa
     1021 tctttttttt ggtttctggt ttactggcag atcaggtcag cgatttaaac ggattcggtg
     1081 agactccctg gagatctcag tacaaggatg tcagagaaaa attcgcatca ctctcctcca
     1141 atccgaactc aagggaaaat gtcaggatac tgaatgaggt tcccaacaga c
//
LOCUS       DAOQGJ010001652         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98489_length_1191_cov_2.651408, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001652 DAOQGJ010000000
VERSION     DAOQGJ010001652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98489_length_1191_cov_2.651408"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK683_20400"
     CDS             complement(<1..186)
                     /locus_tag="PK683_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH10868.1"
                     /translation="MKKILAVLLLIVFQNCDTRKKITDGLEVQLFCSVFASCSSTAVR
                     GVKFGMIGDSWTDLLFGT"
     gene            complement(507..860)
                     /locus_tag="PK683_20405"
     CDS             complement(507..860)
                     /locus_tag="PK683_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HNH10869.1"
                     /translation="MKIYEYKNCGTCRKALKYLDSNKIPYEKIAIRETPPSVSELKKM
                     LKFLDGDSRKLFNTSGGDYRQLNLKERLKEMKTEDQLNLLASNGNLVKRPFVLEKDFG
                     LVGFKEEDWNRVFLK"
     gene            complement(857..>1191)
                     /locus_tag="PK683_20410"
     CDS             complement(857..>1191)
                     /locus_tag="PK683_20410"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNH10870.1"
                     /translation="VYSGYRPSEMQHEFYRRILSQIHAKKIPLIIVWPQSSIPMQNLI
                     QNAKFTPEWKKTVDADSSKYGYRIRNMSDTDRYSCNAFVDGGHIAKDCYYSFIRYVFQ
                     EYYLEYKL"
BASE COUNT          336 a          275 c          220 g          360 t
ORIGIN      
        1 tgttccaaat aaaaggtcag tccagctgtc tccgatcatt ccaaacttga cacctctcac
       61 tgcggtactg ctgcaggaag caaacactga acagaagagc tgaacttcca gcccgtcggt
      121 tattttcttt cttgtatcac agttctgaaa aacaatcaga agcaggacag ccagaatctt
      181 tttcatggat cctccaattt tgttttgtct ttttttcaga aacggtgcaa ggggaaaaat
      241 tcagcagaaa agccgcaaag aacactaaga tgatgctgat cctggaataa aacatcagac
      301 taaaatccaa tacgctgctg acctgcagga tttttccgac ataatacctg tgtccatctg
      361 attcatctgt gattatgccc tttttacaca gtctccgcca ataaagagtt caacacaaaa
      421 accatcattt taaataaaca ctctataaaa accttttgaa gtgtcttcca ttattagact
      481 gagcaggggg aagaatgaaa aaaactttac ttgagaaaaa cccggttcca gtcctcttcc
      541 ttgaagccta caagaccaaa atctttctcc agtacaaaag gccttttgac caggttcccg
      601 tttgaagcca gaagattcag ctgatcctcc gtcttcattt ccttaagcct ttccttcaga
      661 ttcagctgcc tgtagtctcc gcctgacgtg ttgaaaagtt tcctgctgtc accgtccaga
      721 aacttaagca tcttcttcag ttcagaaacc gacggaggag tttctcttat ggcaattttt
      781 tcatagggaa ttttattgct gtcaagatat ttcagtgcct ttctgcaggt tccgcagttc
      841 ttatattcgt agattttcat agtttatatt ccaaatagta ttcctgaaat acatatctga
      901 tgaaactgta gtagcagtcc tttgcaatat gccccccgtc cacgaatgcg ttgcaggaat
      961 acctgtctgt atcggacata ttgcggattc tgtagccgta cttggaagaa tcagcatcta
     1021 cagtcttttt ccattcaggc gtaaactttg cattctgaat caggttctgc atcggaatcg
     1081 aactctgcgg ccagacaatg atcagcggta tcttttttgc atgaatttgg gataaaattc
     1141 ttctgtaaaa ttcatgctgc atttccgagg gtctgtaacc ggaatagacc c
//
LOCUS       DAOQGJ010001653         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98625_length_1190_cov_2.814978, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001653 DAOQGJ010000000
VERSION     DAOQGJ010001653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98625_length_1190_cov_2.814978"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PK683_20415"
     CDS             <1..864
                     /locus_tag="PK683_20415"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH10871.1"
                     /translation="QAFQWAIGLLCAFACLCMYFPVFAIKEKVYCESVPSNVPFWDAL
                     KMTFRNRDFIYFAISDFNYFLALTVLMTGMSYYVTVLLNQPETLTSELMPVMLLSSFL
                     CYPLVNFFARKIGKKNLVIIGFFIFLSVFIGVYFLGSEAVPFSPFVQAALIVIIAGIP
                     AAILGILPNAILADIAELDALKTGNRKEGMFYAARTLMQKFGQTIAGLFFSSLLLVGG
                     GVKKVEKTDAAGNKVLDREKSNLIGVQLTGPVSAGFCALAIFAFMKYREKETLEEIAE
                     IQKKIESGGAI"
     gene            861..1058
                     /locus_tag="PK683_20420"
     CDS             861..1058
                     /locus_tag="PK683_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10872.1"
                     /translation="MSSNRYRIEINKLKNGYIEAKLIDTLTNHPIEFRICDTEQYVQS
                     QISDWQKRFRMNEPKENGNEE"
BASE COUNT          359 a          221 c          264 g          346 t
ORIGIN      
        1 caggcattcc agtgggcaat aggacttctc tgcgcatttg cctgtctatg tatgtatttt
       61 cccgtgtttg caatcaagga aaaggtctac tgtgaatcag ttccttccaa tgttcctttc
      121 tgggacgctt taaagatgac tttcagaaac agagacttta tctatttcgc aatttcagat
      181 tttaactatt ttctcgcttt gactgtgctg atgacaggta tgtcctacta tgtgaccgtt
      241 cttttgaatc agccggagac gctgacaagt gaactgatgc cggttatgct tctttcctct
      301 tttctctgct atcctctggt aaactttttt gcaagaaaaa tagggaaaaa aaatctggta
      361 atcataggct ttttcatttt tctttcagta ttcataggtg tatacttttt agggtctgaa
      421 gctgttcctt tttcaccgtt tgttcaggct gcactgattg taattattgc aggaataccg
      481 gcagcaatac tcggaattct tccaaatgca attcttgcag acattgcaga actggatgca
      541 cttaagacag ggaacagaaa agaagggatg ttttatgcgg cacgaacttt aatgcagaaa
      601 ttcggtcaga caatagcggg acttttcttc agcagtctcc ttcttgtagg cggaggagta
      661 aaaaaagtgg aaaaaactga tgctgctgga aacaaggtat tagacaggga aaaatccaat
      721 ctgatcggag tgcagctgac tggaccggtc tcggcaggat tttgtgcatt ggcaattttt
      781 gcatttatga aataccgtga aaaggaaacc ctggaagaga tagctgaaat acagaaaaaa
      841 attgagtccg gaggggctat atgagcagta atcgatatag aatagaaatt aataagctga
      901 aaaacgggta tattgaggca aagctgatag atacccttac aaatcatccg attgaattca
      961 ggatctgtga cacagagcag tatgttcaga gccagatcag cgactggcag aagagattca
     1021 gaatgaacga accgaaagaa aacgggaatg aagaatagtt cagtatttct gctgatcgca
     1081 tttctcagca cattgcagtt ctgcaatgtg cttccaaaac gaaaattcta ttccaactac
     1141 tttacccccg gctaccgatg ttcaaatgat acagaatggg ataataagga
//
LOCUS       DAOQGJ010001654         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98756_length_1189_cov_3.874780, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001654 DAOQGJ010000000
VERSION     DAOQGJ010001654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98756_length_1189_cov_3.874780"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PK683_20425"
     CDS             <1..833
                     /locus_tag="PK683_20425"
                     /inference="COORDINATES: protein motif:HMM:NF012855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HNH10873.1"
                     /translation="GNCWMISSFAALAEFPDLVQSLFITQEIPDDGRYELQIFDLRTN
                     EWVTVEIDSYIPCLDKTPIYCTSNKNKLWPMLLEKAAAKFCGTYRNFGAYISGLVFPH
                     LTGMRTDVFSREDVEVTDKWQRCVHKFDIENGINNMDGTDEKYSLEELFDLLVEHNEN
                     EDIMTCSSYLYKIENDARGIVGSHVYSLIEAAKAEDVMLLKLRNPWGYGEWEGDWCDS
                     SELWEEYPEVKEALGFEPADDGSFWISLEDFSKRFDDIIICSRGEEIEDSSEYLGDES
                     "
     gene            complement(935..>1189)
                     /locus_tag="PK683_20430"
     CDS             complement(935..>1189)
                     /locus_tag="PK683_20430"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNH10874.1"
                     /translation="LTGLTTLNLERNQIKDISILKDFVHLQALLVSENQIEDITPLMN
                     LSNLTEFQFINNPFFEKLPKNLQQQEPLDALHQLQKKADK"
BASE COUNT          345 a          212 c          282 g          350 t
ORIGIN      
        1 taggaaactg ctggatgatc agttcatttg ccgccttggc cgagtttcct gatttagtgc
       61 agagcctgtt tattactcag gagattccgg acgacggcag atatgaactt cagatctttg
      121 atctgagaac aaatgaatgg gtgactgtgg aaattgattc ttatattccc tgtctggata
      181 aaaccccgat ctactgtact tcgaataaaa acaaactttg gcctatgctc cttgaaaagg
      241 ctgctgcaaa gttttgcggt acatatagaa atttcggagc ttacatttcc gggcttgtct
      301 ttcctcatct gacaggaatg cgcaccgatg ttttttcaag ggaagatgtt gaagttactg
      361 ataaatggca gcgctgcgtt cataagttcg atatcgaaaa tggaataaac aatatggatg
      421 gaactgatga gaagtacagt ctggaggaat tattcgacct tcttgtcgaa cacaatgaaa
      481 acgaagatat tatgacttgt tcatcctatc tttacaagat tgaaaacgat gcaagaggaa
      541 ttgtcggttc acatgtttat tcactgatag aagcagccaa agcggaagat gttatgctgc
      601 tgaaattacg caatccatgg ggatatggag aatgggaagg agactggtgt gacagttcag
      661 aactttggga agaatatccg gaagtgaaag aggctcttgg atttgaaccg gccgatgacg
      721 gatccttctg gatttctctg gaagattttt caaaacgttt tgatgatata atcatctgca
      781 gcagaggtga ggaaattgaa gattccagtg aatacttagg cgatgagtct taatggaata
      841 ttaatgatcg gatttgaaat caatagacag gaatttacct gcttcagacc ccgcactcta
      901 ttgctggcct gatgaagaca ggtattctga aatgttactt atcagctttc ttctgcagct
      961 gatgcaatgc gtccaaaggt tcctgctgct gcaggttttt aggaagtttc tcaaagaagg
     1021 gattattgat aaactgaaat tctgtcagat tcgacaggtt catcagcggt gtgatatcct
     1081 ctatctggtt ttcgctcaca agcagggcct gcaagtgtac aaaatctttc aaaatagaaa
     1141 tatccttgat ttggttgcgt tccagattca gggtagtcag accagtcag
//
LOCUS       DAOQGJ010001655         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98760_length_1189_cov_3.722222, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001655 DAOQGJ010000000
VERSION     DAOQGJ010001655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98760_length_1189_cov_3.722222"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PK683_20435"
     CDS             complement(<1..720)
                     /locus_tag="PK683_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004763882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH10875.1"
                     /translation="MSISSLELKKLTAKFDSKLKEIISEDLPILKKIKKHVIESGGKR
                     IRPLTHYFLSQMLGYSGNSWADIGSIAELIHGASLLHDDVVDGAELRRGVPTVGKLYG
                     NKQAILGGDYLLACGIDHLNKFKNPELMSAFTSVIRDLSVGELIQMEWEKSPKITRKI
                     YEKVIYGKTASLFGAAALTAGILAETSGRQKEILKEFGINMGMLFQMKDDYIDYFEKK
                     DKSGKIPLKDFQNGLYTYPVIL"
     gene            871..>1189
                     /locus_tag="PK683_20440"
     CDS             871..>1189
                     /locus_tag="PK683_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH10876.1"
                     /translation="MQILTELYHSIYFNFFSFGTLLATVFMLVCGVFLMKVPKMQRST
                     FHLALSYILFSIFYFGYFTAAVFYHPLAAYHRWVTVGFILLLEIHILQWFLRYPRTTH
                     PKFA"
BASE COUNT          300 a          278 c          230 g          381 t
ORIGIN      
        1 cagaatcacc ggataagtgt aaagaccatt ctggaaatct ttcaatggaa tctttccgct
       61 tttgtctttc ttctcaaaat aatcaatata gtcatctttc atttgaaaga gcattcccat
      121 attaattccg aattctttga ggatctcctt ctgtcttccc gatgtttcag ccagaattcc
      181 cgccgtcagt gcggctgcac cgaacaatga tgcagtcttt ccataaatta ctttctcgta
      241 gatttttctg gtaatcttgg ggcttttctc ccattccatc tgaatcagtt ctcctacaga
      301 aagatctctg ataacagagg taaaagcgct catcagctcc ggatttttaa acttgttcag
      361 gtgatctatg ccgcaggcga ggagataatc tcctccaaga atcgcctgct tgttcccata
      421 aagttttccg actgtgggaa ctccacgcct gagttcggct ccgtcgacta catcgtcatg
      481 caggaggctt gcaccgtgga tcagttcagc gatggaaccg atatcggccc aggagttccc
      541 actatagccg agcatctgag agagaaaata atgagtgagg ggccggatcc ttttaccgcc
      601 cgactcaata acatgttttt ttattttttt cagaatcgga aggtcttccg aaatgatttc
      661 cttaagtttg ctgtcaaact ttgctgtcag ttttttcagt tccagggagg atatagacat
      721 atattacgat acgctctgaa tcacattctg atgtaaagga aaattcccct attctaatct
      781 tcctatgccg ttctgccgat cgaaaaaggc tttacagact gaagatggaa actaacatgg
      841 ttcagacgtt tacaggggca caactgaaca atgcagatcc ttacagaatt atatcactcc
      901 atttatttca attttttctc tttcggtact ctcctggcga cagtgttcat gctggtctgc
      961 ggtgtatttc tgatgaaggt tccaaaaatg cagagaagta catttcatct ggcactttct
     1021 tatatccttt tcagcatatt ttatttcggt tattttacag cagcagtatt ctaccatcct
     1081 ctggccgcat atcatcggtg ggtcactgtc ggattcatac ttcttctgga gattcacatt
     1141 ctgcagtggt ttctccggta tcccagaaca actcatccaa agtttgcca
//
LOCUS       DAOQGJ010001656         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98765_length_1189_cov_3.499118, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001656 DAOQGJ010000000
VERSION     DAOQGJ010001656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98765_length_1189_cov_3.499118"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PK683_20445"
     CDS             complement(<1..1147)
                     /locus_tag="PK683_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10877.1"
                     /translation="MMAASTDLSNVTASMNAVPAPPFPINFIWDAIKSIAGNGAMIVR
                     GVVDAILQDVLLTALPYVKPFLIEGMLSDFVTKVAGNLFNAILYPLGKGIYLTMPTIV
                     PDPIGKFKLNLAVGITPNSTGLVQIDAGSGSKGIVAPVDLKLTGINTLLTGSGYNYSC
                     AASDVTAEKCPPAPAGNASGQGSFVLSRPSSKWNTALPSPLKYSAANPGVLLGVHPDA
                     IGQFFFGLWKNGGLNMRIDNAFLTKLSLYRLETKNEPIRLVRELLKAKNLFAVLIPSG
                     VTDPALTAPVIYKAKNSGGTVQTVEDTDEIVIKLRPLQAPYVTVDPPPAGAVYIPKLN
                     ITLSDVEVQIFGKRGLTEYRVLAFRLNLNTRAIFNLGGFQGNSEVIPN"
BASE COUNT          339 a          275 c          257 g          318 t
ORIGIN      
        1 ggtttggaat gacctcagag tttccctgaa atccccctaa attgaatatc gcacgcgtat
       61 tcagatttag cctgaaagca agcaccctgt attccgtcag tccgcgtttc ccaaaaatct
      121 gcacctccac atcactcaaa gtgatattta attttggaat atatactgct cctgcaggcg
      181 gaggatctac agtcacataa ggggcctgaa gaggacggag tttgattacg atttcatcag
      241 tgtcctccac tgtctgcact gttccgccgg aattcttcgc tttgtaaatt acaggagcag
      301 taagagcagg atcagtcact ccggatggaa tcagaaccgc aaaaagattc ttcgctttca
      361 gaagttctct tacaagacga atcggttcat tctttgtttc caatcggtat aatgataatt
      421 tagttaaaaa tgcattatca atcctcatat tcagtcctcc gtttttccag agcccgaaga
      481 aaaactgtcc tattgcgtca ggatgaacac caaggagaac acctggattt gcagcactgt
      541 acttaagcgg gctggggaga gctgtgttcc atttagaact gggtctggac agaacgaaag
      601 atccctgacc ggaagcattg cctgcaggag caggagggca tttttctgca gtaacatccg
      661 atgcagcaca gctgtaatta tatccggatc cggtaagaag tgtatttata cctgtgagtt
      721 ttaagtctac aggagcaaca atacctttgg aaccggaacc tgcatcaatc tgcacaagtc
      781 cggtagaatt tggagtgatt ccgacagcca gattcagttt aaatttccca atcggatcag
      841 gaactatagt cggcattgta aggtagatcc cttttcctaa aggatacaga attgcattga
      901 aaagatttcc ggcaacctta gtgacaaaat cggaaagcat accttcaatc aggaagggct
      961 ttacataagg aagtgcggta aggagcacat cctgcagtat tgcatcgact acccctctta
     1021 caatcattgc accgttcccc gcaatacttt ttattgcatc ccatataaaa tttattggaa
     1081 acggcggagc aggcacagca ttcattgaag cggttacgtt gctcagatct gttgatgctg
     1141 ccatcataag aggatttatc agcagtcctg atccgacacc tccaagccc
//
LOCUS       DAOQGJ010001657         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98866_length_1189_cov_1.675485, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001657 DAOQGJ010000000
VERSION     DAOQGJ010001657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98866_length_1189_cov_1.675485"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PK683_20450"
     CDS             complement(<1..337)
                     /locus_tag="PK683_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNH10878.1"
                     /translation="MYYLGRLIGLVLFIFLIVPMRIVYGRKKCRYIGTEKLSGLQGRS
                     FFVAANHIKPRSRFLKFISMPYDAYIARRMFLEFGFYSTALTSYDSPANPKSAFGKFF
                     TEKVKEQLTK"
     gene            949..>1189
                     /locus_tag="PK683_20455"
     CDS             949..>1189
                     /locus_tag="PK683_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10879.1"
                     /translation="MIKKIYIILIAFSFFSCFFHRKFDKVETVVKTDRLKIDKKVRIA
                     LSEKSRFLQRDTISLGYNFFSPKYDNVRNSEIDRLL"
BASE COUNT          394 a          215 c          233 g          347 t
ORIGIN      
        1 ccttagtcag ctgctccttc actttttcag taaagaattt tccgaatgca gattttggat
       61 ttgcaggaga gtcgtagctt gtgagagcag tgctgtagaa tccgaactcc agaaacattc
      121 ttcttgcaat atacgcatcg taaggcatac tgatgaattt cagaaaacgg cttctgggct
      181 tgatatggtt tgccgcaaca aaaaaagatc tgccctgcag accggaaagt ttttcagtgc
      241 ctatgtatct gcattttttt ctgccgtata caattctcat tggaacaatt aaaaaaataa
      301 acagcacaag tcctatcagt cttcccaagt aatacatatc agctcctgag tttcggtttt
      361 ctccgaatct gaaacataag atgaacaagc ttcagaaaag ttgcaaaata atgaaggcgt
      421 aactgaaact gagtaaaaga atcgtttcat cgaaagttca ccctgttatc tgaattcaat
      481 agttggaaat tccttctgtc attattccgt tctatgcaaa aagcagtcga ctgtatttta
      541 attgacattt aagctatgaa agtaagctga atgaattttc agatatattc tttttaaaag
      601 attgggtcgg agcctaaaaa gcgatgagtt attcatacta aaatatgacg aacaggaacg
      661 ggtacgtcct tacggatgat cgaaatgaat acagtatcat tataatacag ttgcaagtcg
      721 gaccttcctt gcaccgtcgc ttaaagcagg atcagtaagg ctcaagtcct ctgaaagaaa
      781 ggaatcgaag tggatgacac gagccagcca agcagtgttc agtcacaatt actggttgag
      841 aatatacaaa cgtgaataca gcgagctccc aaatgatcca actggacaaa aatgaacaga
      901 tcttcagaac tggatgatta tgggttatct tgcggggcgt acttataaat gattaaaaaa
      961 atatatatta tccttattgc attctctttt ttttcctgct tttttcacag aaagtttgac
     1021 aaggtggaaa cagttgtgaa gactgatcgg cttaaaatag ataaaaaagt cagaatagca
     1081 ctcagtgaga aatcaagatt tcttcaaagg gatacgatca gtcttggata caattttttc
     1141 agtccaaaat atgacaacgt cagaaacagt gaaattgatc gattgctgt
//
LOCUS       DAOQGJ010001658         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_98969_length_1188_cov_2.401589, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001658 DAOQGJ010000000
VERSION     DAOQGJ010001658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98969_length_1188_cov_2.401589"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK683_20460"
     CDS             <1..190
                     /locus_tag="PK683_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10880.1"
                     /translation="TSEEGEPYMEKYMQKYLGRFLNLGRYWIARVESGDVSQYILVSS
                     VTFLFSIFLIFIFRNYFL"
     gene            199..1071
                     /locus_tag="PK683_20465"
     CDS             199..1071
                     /locus_tag="PK683_20465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNH10881.1"
                     /translation="MDYIRYIPLLLFFTLFPVLIPGMVKKVRARCQGRKGPVILQPWY
                     DIRRMMKKTPVDGPNSGIYAEVAPLVSFLAALTAWSIPVFEWTSFILIPFFLVLQRIT
                     LLAFAMETGTSFGGLGSSREVLLYVLGEPVLVIAIIVSQSSLQVEFSVMGIALGILFL
                     AACSVIMLAEMAKPPFDDPRTHLELTMVHEAMLLEASGKTMAFFEIASILKMAALITL
                     LMRIAFEHINLSSLGIAGKFLEEIIMILGALVVPAMIGWWEAVSVRRKWEWIPEFMGM
                     TLIFILALGTIVRL"
BASE COUNT          293 a          233 c          276 g          386 t
ORIGIN      
        1 cacttcggaa gaaggcgagc cttatatgga aaaatatatg cagaagtatc tcggaagatt
       61 tctgaatctc ggaagatact ggattgccag ggtggaatcc ggcgatgttt cgcagtacat
      121 tcttgtttcg agtgtcactt ttctgttttc aatatttctg attttcatct tcagaaacta
      181 ctttttatag gacattatat ggattacata cgatatatcc cgttattgct gttttttact
      241 cttttccctg ttctgattcc cggaatggtc aagaaagtca gagcaaggtg tcaggggaga
      301 aaaggtcctg tcatccttca gccctggtat gatatcagga ggatgatgaa aaaaactcct
      361 gtggatggac ccaattcagg aatctatgca gaggtggcac ctcttgtgtc atttctggcc
      421 gcacttacgg catggtcgat tcctgtcttt gagtggacat ccttcatact gattcctttt
      481 tttcttgtgc tgcagagaat tactcttctg gcatttgcta tggaaacagg tacatccttc
      541 ggaggtctgg gaagttctag agaagtcctt ctttatgttc tgggggaacc ggttctggtc
      601 attgctatca ttgtctctca gtcgtctctt caggtggaat tttccgtcat gggaattgcg
      661 ctgggaattc tttttcttgc agcctgttct gtcattatgc ttgctgaaat ggccaagccc
      721 ccttttgacg atccgagaac acatcttgaa ctgacgatgg tgcatgaagc aatgctcctt
      781 gaagcgtccg gcaagacaat ggcatttttt gaaatcgctt ccatactgaa aatggctgct
      841 ctgattactc ttctgatgag aattgccttt gagcatataa atctttcaag tctcggaatt
      901 gcaggaaaat ttctggaaga aatcattatg attctgggtg cacttgtcgt tcctgcaatg
      961 atcggctggt gggaggcagt cagtgtcaga agaaaatggg aatggattcc tgaatttatg
     1021 ggaatgacac tgatattcat acttgccctg ggcacaattg taaggctgta ggagagatca
     1081 tgcaaagtta tatacatctt atttatctgg tgcttcttct taccggggtc atgatactca
     1141 ttgaaaacag acttaaaaga atcatctacc tgatcggttt tcagggat
//
LOCUS       DAOQGJ010001659         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_99089_length_1187_cov_3.090106, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001659 DAOQGJ010000000
VERSION     DAOQGJ010001659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99089_length_1187_cov_3.090106"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK683_20470"
     CDS             <1..468
                     /locus_tag="PK683_20470"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNH10882.1"
                     /translation="DGLSLAWAIVENLSKSHFDGNRTKTIFATHYHELTDLEKEAGVF
                     NLFMDTVEKEGNVIFLKKARMGKAKKSFGIYVAKLAGIPDSIVQRASEILSGLENKKK
                     EIKFKDSTPSLFEFAEKPDEGSVLKKEILSIPLDEISPRQAQEILYAIQKRLK"
     gene            552..>1187
                     /locus_tag="PK683_20475"
     CDS             552..>1187
                     /locus_tag="PK683_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10883.1"
                     /translation="MTGFRKSTIFLALLGVAGLAAQAKKPIPSNSSVDKASEVIDNII
                     NTKDTVQPVKREETNTSTTTTTTTTTTTVKPATTSTSPASTSTTTTAPTSTTTVKPAT
                     TSTAPSSTAPASTSTAPATSSPTAVPASIQPSGKSALFVNSKQTFELLPVDDLSSVDY
                     VEYKINDGDYVKYTGPITLGKEGPATIAYRAIDKVGNRENTQVLSVIVDNTP"
BASE COUNT          383 a          276 c          238 g          290 t
ORIGIN      
        1 gacggtctga gtctcgcctg ggcgattgtg gagaatcttt cgaagtccca ttttgacggg
       61 aacagaacaa agactatctt tgcgacacat tatcatgaac tgactgatct ggaaaaggaa
      121 gcaggagtat ttaatctttt tatggataca gtcgaaaaag aggggaatgt catcttcctg
      181 aaaaaagcca ggatgggaaa agccaaaaag tctttcggga tctatgtggc caaactggca
      241 ggcatccctg attcgattgt gcagagagcc tcagagattc tttcaggtct cgaaaacaag
      301 aaaaaggaaa tcaaattcaa ggattccact ccctctcttt ttgaatttgc ggaaaagccg
      361 gacgagggga gcgttctgaa aaaggaaatc ctctcgattc ctctcgatga aatcagtcca
      421 agacaggctc aggaaattct gtatgcaatt cagaaacgtc tgaaatgatt tttcccgtct
      481 ctcttgacaa gcattcggat taacaggaaa atggctatta gtaaccatac agtccaaatt
      541 aggagaaacc aatgacagga tttagaaaaa gcactatttt tctggctttg cttggcgtgg
      601 caggtcttgc tgctcaggca aagaaaccta tcccttccaa ttcatccgta gacaaagctt
      661 cggaagtaat agataacatt atcaatacta aagatactgt tcagccggta aaaagagaag
      721 aaacaaatac ttctactaca acaactacaa ctaccactac aacaacagtc aagcctgcga
      781 caacttcaac atcaccggct tcgacatcca ctacaacaac agcaccgact tccacaacaa
      841 cagtcaagcc tgcgacaact tcaacagcac cttcttctac agctccggca agcacttcca
      901 ctgcgcctgc tacttcttcc ccgacagctg ttcctgcttc cattcagcct tccggaaaat
      961 cagctctgtt tgtaaattca aagcagactt tcgagcttct tcctgtggat gatttaagct
     1021 cagtggatta tgtggaatac aaaattaatg acggcgacta tgtaaaatat accggtccaa
     1081 tcactctagg aaaagaaggt cctgcaacaa tcgcttaccg tgcaattgac aaagtaggaa
     1141 acagagaaaa cactcaggta ttgagcgtaa tcgtggacaa tactcct
//
LOCUS       DAOQGJ010001660         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_99209_length_1187_cov_1.170495, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001660 DAOQGJ010000000
VERSION     DAOQGJ010001660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99209_length_1187_cov_1.170495"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK683_20480"
     CDS             <1..407
                     /locus_tag="PK683_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10884.1"
                     /translation="NINNKIILVSNISNSIPQVIVCNPSLSGCIGNDYPNILAMAEIT
                     SSVIDPIHNKIYFFGRDVYDESKMRMFRCEINGTACTNRNISDLTGTTGNGYYPDAVV
                     DTVSKKIYAVSENRSTNNSISYWSIPLYIEDP"
     gene            894..1163
                     /locus_tag="PK683_20485"
     CDS             894..1163
                     /locus_tag="PK683_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10885.1"
                     /translation="MRKTLFRIIIMLLMMFQSVQCSWKNSGNLFDPESIVGPFLGLDP
                     FKVTPSKSKITVSGIASSIAEGIGPENSEEKKSRENISAGVRIWY"
BASE COUNT          397 a          198 c          234 g          358 t
ORIGIN      
        1 acaatataaa taataaaatt attcttgttt ccaatatttc gaacagtatc ccgcaggtga
       61 tagtctgcaa cccctctctc tcgggatgta taggaaatga ttatccaaat atattggcta
      121 tggcagaaat tacatcttct gtcattgatc caattcataa taagatttat ttttttggaa
      181 gagatgttta cgatgaatca aaaatgcgga tgttccgctg tgaaataaac ggaacagcct
      241 gtacaaatag aaatatttct gatctgactg gaactacagg taatggctat tatcctgatg
      301 ccgtagtcga tacggtgagt aaaaaaatat atgcagtttc tgaaaaccga tcaacaaata
      361 acagcatttc atactggtca attcctctgt atatagaaga cccttgaatg aactgaaatt
      421 caggccggag aaaacattcc gaatcggaag acagaggaac tttataaaat cagaatttaa
      481 ctggcggcgg cacagctcag attgaaagac agagagaaac tgcctttcaa tcacttttat
      541 tctgctgcgt actgatactt gccttaaaaa ttctgctcag cagtaaagtc agttcagcaa
      601 acttaagacg ggactgcagt tttgtagtag ttttcaccta cagatggtca taaatttctg
      661 tataatagta attttttatt tacagattga gaatcaattc ccttatgaaa aatggcatca
      721 ttacgaaagt ataaaaaata caaagattta ttctgtgcga cctatcaaaa ttgacaggtt
      781 tctgcaggaa ggtcagtaat ttttttctga aaactgtctg ttttgataaa aaatacccaa
      841 tattgggtat atacaaactt ttcagagtac tgtagtattg gataaactgg gatatgagga
      901 aaaccttatt tagaataatt ataatgcttc tgatgatgtt tcagtctgta caatgttctt
      961 ggaaaaattc cggaaatctt tttgatccgg aaagcattgt aggtcctttt ttaggactcg
     1021 acccttttaa ggtcactcct tctaaaagca aaattacggt gtctgggatt gcgagttcca
     1081 ttgcagaagg tatcgggccg gagaacagtg aggaaaaaaa aagcagggaa aacatatcag
     1141 caggcgtgcg gatctggtac tgatggactg actatgctta gaacagg
//
LOCUS       DAOQGJ010001661         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_99229_length_1186_cov_4.177719, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001661 DAOQGJ010000000
VERSION     DAOQGJ010001661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99229_length_1186_cov_4.177719"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1117)
                     /locus_tag="PK683_20490"
     CDS             complement(170..1117)
                     /locus_tag="PK683_20490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNH10886.1"
                     /translation="MKNSDPMSMKTKIILNPKSGDGKVAKNWELIQSELRRHNFKYDT
                     EYTTAQDSATSMARKALKEGYERIIAVGGDGTLSETVNGFFENGRQINPDAVFSFIMS
                     GRGNDFRKTFGISKDLKEAVDRCVNGKVRKIDLVRIDHQNHSDEKVVRYFMNIASIGM
                     GGEVASRVNQSKLGKYIGGKGVFYYESLMALLSYKNKNVRIRIDDTFDQKLKIRLICI
                     ANGQYAGGGMWFAPHADISDGILEVMILKDVSKLTSFGMTGTIYKGKLKDHQDVITLR
                     GKKITVESDEKVLLEVDGEVPGTLSAAYEVLPSCLNLQI"
BASE COUNT          292 a          267 c          219 g          408 t
ORIGIN      
        1 atgctttctg aaaattacag gatctagata gcatatcgta tttgcgtaac gtgagattgt
       61 aatataatta ttgatttatc gaaagggaga ccgttctgta tagtccaagg cagggagacc
      121 ccgtccatat ttaactgaac gagatctgaa cacagaatca gtcgtaattc tatatctgaa
      181 gattcaggca ggagggaaga acttcatatg ctgcagagag tgttccggga acttctccgt
      241 ccacttccag aaggactttt tcgtctgatt ctactgtaat tttttttcct ctaagtgtaa
      301 ttacatcttg atgatctttc agttttccct tgtagatggt gcctgtcatt ccgaatgatg
      361 tgagtttcga aacatctttc agaatcatca cttccagtat tccgtccgaa atgtctgcat
      421 gaggagcaaa ccacattcct ccgcctgcat actgtccatt tgcaatgcag atcagtctga
      481 tcttgagttt ctgatcaaat gtatcatcta tcctgattct tacatttttg tttttatagg
      541 aaagaagagc catcaaactt tcataataaa aaactccttt gcctcctatg tattttccca
      601 gcttggactg attgacccga cttgccactt caccgcccat tcctatgctg gcaatgttca
      661 taaaatagcg tacgactttt tcgtctgaat ggttctgatg atcgatcctg accagatcta
      721 tttttctgac ctttccattt acgcacctgt ccactgcttc tttcagatct ttggagattc
      781 cgaaagtctt gcgaaaatca tttcctctcc cgctcataat gaatgagaat actgcatctg
      841 gattgatctg tcttccattc tcaaaaaacc cgtttactgt ttcactgagg gttccgtctc
      901 ctccgactgc aatgattctt tcgtatcctt ccttcagtgc ttttctggcc attgaagttg
      961 ctgagtcctg tgcagtggta tactctgtat catatttaaa attatgacgg cgcagttcag
     1021 actgaatcag ttcccaattt ttggctactt tcccatcgcc cgatttgggg ttcaggatga
     1081 tttttgtttt cattgacatc ggatccgagt ttttcatgga cactctctaa taaaatggat
     1141 tttatgcaga tttatcaaag ggactgcctc agccgtccgt ttcatt
//
LOCUS       DAOQGJ010001662         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_99270_length_1186_cov_2.900973, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001662 DAOQGJ010000000
VERSION     DAOQGJ010001662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99270_length_1186_cov_2.900973"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK683_20495"
     CDS             complement(<1..444)
                     /locus_tag="PK683_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10887.1"
                     /translation="MKLKRLLLLFMMIPLYFCSQTKKCKHINPADSECYTQMMGLCSA
                     LGNGQVQYNYNTFYEPWKFYFNNTVVGIYTKYSSDDSYILYTENSGQTWEKISVGDSL
                     MENVFVHCLQGSAVCYASARKTGKYFIMKSIDAGKTFSVIFEKSTP"
     gene            complement(724..>1186)
                     /locus_tag="PK683_20500"
     CDS             complement(724..>1186)
                     /locus_tag="PK683_20500"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH10888.1"
                     /translation="SDLVHLLNDLFTEFDRLCSKHNAEKIKTIGDAYMAVSGLPVPDK
                     NHAETLYLLAKDIIEYMPIFNSSHGTELSVRIGINSGSVTAGVIGTQKISYDLWGDAV
                     NTASRMESSGIPGKIHISESAYILLKDKYSFEERGEIEIKGKGKMKTYLGI"
BASE COUNT          343 a          241 c          231 g          371 t
ORIGIN      
        1 aggagttgat ttttcaaaaa tgacggaaaa agtctttccg gcatctatag atttcatgat
       61 aaaatatttt ccggttttcc gtgctgaagc ataacaaact gcagatccct gcagacaatg
      121 tacgaaaaca ttttccatca gagagtctcc gactgaaatt ttttcccagg tctgaccgga
      181 attttccgta tagagaatat aggaatcgtc agaagaatac tttgtataaa taccgacaac
      241 ggtgttatta aaataaaatt tccaaggttc ataaaaagta ttataattgt attgaacttg
      301 tccatttccg agtgcagaac aaagtcccat catctgagtg taacattccg agtcggcagg
      361 gttgatatgt ttacactttt ttgtctgact gcagaaatag agaggaatca tcataaagag
      421 aaggagcagt cttttcagtt tcatatcggc agaatgaagg ttttctcaga attgtcaatt
      481 tatttttcag atatattctt ttccgctgca gtattacttc aatttcgctt ttcatactgc
      541 aattgcaata tccacaaaac gttctctgga catctctgca ccgtaagcta tgcaggcata
      601 tatgtctgcc tcaacaagtc cgggatagtt ttctaagaca gactgtatag tttctcccga
      661 agccgccaga ggctaaaaaa tccaagatta aagaaaccca tattctgtga cctgtatcta
      721 ggttcaaatc ccaagatacg tcttcatttt tccttttccc ttgatctcaa tttctcctct
      781 ttcttcaaag gaatatttat ccttcagaag gatatacgcg gattcagaga tatggatttt
      841 tcccggaatt ccggaggatt ccattcggct ggctgtgttg actgcatctc cccagagatc
      901 atagctgatt ttctgggtgc cgatcacgcc ggctgtcact gaacctgagt tgatcccgat
      961 ccgcacactg agttctgttc cgtgagagga attgaaaata ggcatatatt caataatgtc
     1021 ttttgcaaga agatataatg tttctgcatg atttttatcc ggaacgggaa ggcctgacac
     1081 agccatatag gcatccccga ttgtcttgat cttttctgca ttgtgctttg aacagagtct
     1141 gtcaaattca gtgaagagat cattcagaag atgcacaaga tcggag
//
LOCUS       DAOQGJ010001663         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_99293_length_1186_cov_2.482759, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001663 DAOQGJ010000000
VERSION     DAOQGJ010001663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99293_length_1186_cov_2.482759"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..650
                     /locus_tag="PK683_20505"
     CDS             255..650
                     /locus_tag="PK683_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10889.1"
                     /translation="MKKISISLLPVLFFVIACGEEKKPEPATATATATATAEAPKETP
                     KPANDFSQKIKEYEGFVAKFCALSEKMKGAGAMEKVTLAADFAKEVSTFNALTTDISS
                     IKASAAEDQKKQIESATARGASCAKAAAL"
     gene            739..1023
                     /gene="gatC"
                     /locus_tag="PK683_20510"
     CDS             739..1023
                     /gene="gatC"
                     /locus_tag="PK683_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNH10890.1"
                     /translation="MDGKKLKEIAYLARLKIEEGEMDSMLRDFNSIQEYVDHVKELDT
                     SSVTEEDIYFHVKNFTRPDKTGESLNREKIAAVAPAYENGYVIVPKVIET"
BASE COUNT          375 a          235 c          253 g          323 t
ORIGIN      
        1 tcactgcaga caggcggatt agatacagac tgatgaacac aaggccggcc agtaccgaat
       61 cagggcagtt tttatttgat tcggttcaat tatttcagtt gttcacggtt cctctgtatc
      121 cggataagga gtctgagtgt acaattcaga tcgggcagaa attgaatatt ttgtattgac
      181 ttagatcaag aacgattcaa atttgaatca ggagagatca aaaaatcttt ccaattttaa
      241 attcaggatt acgcatgaaa aaaatttcaa tctccttact cccagttttg ttttttgtca
      301 ttgcctgcgg cgaagaaaaa aagcctgagc ctgcaacagc taccgcaaca gctactgcga
      361 cagcagaggc gcctaaagaa actccaaaac ccgctaatga tttttcccag aagataaaag
      421 aatacgaagg atttgttgct aaattctgtg ctctttccga gaaaatgaaa ggtgccggtg
      481 ccatggaaaa agtgaccctt gctgctgatt ttgcaaaaga agtttccact ttcaatgcat
      541 tgactaccga tatttcttcg atcaaggcaa gtgctgctga agatcagaaa aaacagattg
      601 aatctgctac tgcaagaggt gcttcctgcg caaaagctgc ggctctttaa tgtaaacttt
      661 gaggagactg agttctcctc attttctttc ctctgaaatt ctcttgcatc cccgtaccca
      721 gtacagacct tagctaatat ggatggaaaa aagctcaagg aaattgccta tctggctaga
      781 cttaaaatag aagaagggga gatggattcc atgctccgcg atttcaacag cattcaggaa
      841 tatgtggatc atgtgaagga actggacacc agttcggtca cggaagaaga catttatttt
      901 catgtaaaga acttcacgag acctgataaa acaggtgagt cattgaatcg tgaaaaaatc
      961 gcagctgtcg ctcctgcata tgaaaatgga tatgtaattg ttccgaaggt aattgaaaca
     1021 tgattttttc cagttatact gaaattaaag caggactgaa cagcggtgca atttctccgc
     1081 ttgatctggc aaaaagctgt ctgaaaagaa tagaagaaac agaacccaaa ataagggcgt
     1141 tcatccattt ttctcctgaa agagttttgg ctcaggctga agcctc
//
LOCUS       DAOQGJ010001664         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_99789_length_1183_cov_2.034574, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001664 DAOQGJ010000000
VERSION     DAOQGJ010001664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99789_length_1183_cov_2.034574"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK683_20515"
     CDS             <1..398
                     /locus_tag="PK683_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10891.1"
                     /translation="LGIQSFGFVQLTETERGEEEVLIFTEEGSEGTAEVHGSVTEAEV
                     PEGKRDFRCRGFLLRQDIQKIRRNSISRILRFRETASDLKIKLSVLFLCIYSFIIINV
                     ILFITVKVSRIYFGLTPLMACAVKGSSGQ"
     gene            complement(341..>1183)
                     /locus_tag="PK683_20520"
     CDS             complement(341..>1183)
                     /locus_tag="PK683_20520"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HNH10892.1"
                     /translation="NDPMTEKEFICAAEITGDGTDNRIRRSAAVQLTDLLKYFGHLIK
                     KEFRLALFQNERLKKSEVQKILELEISEKEISEISEEECSEFFSSLIRKEGISFLNPK
                     DDFREIQKRSEFLRLSGADFPELSDSFLSDNPNLWIEPYLYGIRRVSELKKISLLSVI
                     SEYLGRKNLSILEKEAPEKLLLPSGSRAKIFYSGTEARISARLQELFGLLDTPRLAFS
                     KSPVVFEILSPSMRPVQVTSDLRNFWTKTYFDVKKELKGEYPKHYWPEDPFTAQAIRG
                     VRPK"
BASE COUNT          331 a          236 c          261 g          355 t
ORIGIN      
        1 ctttaggaat acagtccttc ggatttgtcc agctcactga aacagaaaga ggagaagaag
       61 aagtgctgat ttttacggaa gagggttctg aaggaactgc agaagtgcat ggatctgtga
      121 ctgaagcgga agttccggaa ggaaaaagag atttcaggtg cagaggattt cttttgagac
      181 aggatataca gaaaatcaga agaaacagca tcagcagaat actccggttt cgggagacag
      241 catccgatct gaaaattaag ctttctgttc tgtttctgtg catatactct tttataatca
      301 ttaatgttat actgttcatt acagtaaaag tttcaagaat ttattttggc ctgacgcctc
      361 tgatggcctg cgcagtgaag ggatcttccg gccagtaatg cttagggtac tcacccttca
      421 gttctttctt cacatcgaag tatgttttgg tccagaaatt ccgcagatct gaagtgacct
      481 gaacaggtct catggaaggc gaaaggattt cgaagacaac aggcgatttg gaaaaggcaa
      541 gccttggagt gtccagaagt ccgaaaagtt cctgaagtct cgcggaaatt ctggcctcag
      601 ttcctgaata gaagatcttc gccctgcttc cgctgggcag aagaagtttt tcaggggctt
      661 ctttttcaag tatgctgaga ttctttcttc ccaggtattc cgaaatcact gacagaagag
      721 aaatcttctt aagctcagag actcttctta ttccgtacag ataaggttct atccagagat
      781 tcggattatc tgaaagaaat gaatctgata attcagggaa atcggccccg gacagtcgga
      841 gaaattctga tcttttctga atctctctga agtcatcctt aggatttaaa aaggaaattc
      901 cttcttttct gatgagggaa gagaagaatt ccgaacattc ttcttctgat atttctgaaa
      961 tttccttttc agagatttcc agttccagaa tcttctgcac ttctgatttc ttgagtcttt
     1021 cattctgaaa cagggcaagc ctaaattctt ttttgataag atgcccgaaa tacttcagaa
     1081 gatcagtcag ctgaactgct gcagatcttc tgattctgtt gtctgtaccg tccccggtaa
     1141 tctccgcagc acagatgaat tctttttccg tcataggatc att
//
LOCUS       DAOQGJ010001665         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_99836_length_1182_cov_4.381544, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001665 DAOQGJ010000000
VERSION     DAOQGJ010001665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99836_length_1182_cov_4.381544"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1182)
                     /locus_tag="PK683_20525"
     CDS             complement(<1..>1182)
                     /locus_tag="PK683_20525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNH10893.1"
                     /translation="GVSKTEVDGVVAGEISQSAKAPNIARVISVKIGIPLEASAVTVA
                     NNCVSGTEAVLDAARRIMIGEGDFYLALGMETMSQGPFIIEGAKENSKTATVDKLMAN
                     WADAPALGVKVYDSIDEGLNDPIRKLNMAMTGELAAQNYDLTKKELDDYAYMSYKKAY
                     DSIKAGKYRPYQVSVKLTDGSLLEDDEYIMSKGGMVENPGRFDKAGVIFDSKFMSLKE
                     FYEKYGEWINKKYEEGKTQAAVTLFNACPRSDGAGAIFVTTESKAKKLGLKIQAYLRG
                     WGMYGVDPAHMGIGMAFSMNKALTNAGLKFTDIDNFEIHEAFAATAMGSFREVKKSWG
                     FDLEAANKAGKVNPNGGTLAIGHPLGATGIRVLLNQIMDFDLNPKNKLSLNAICAGGG
                     V"
BASE COUNT          276 a          301 c          235 g          370 t
ORIGIN      
        1 caacaccgcc cccggcacag atcgcattca gagaaagttt gttttttgga ttcagatcaa
       61 agtccataat ctggttcaga agtacacgga ttccagttgc tcccagcggg tggccgattg
      121 caagggttcc tccgttagga ttgacctttc ctgccttgtt cgctgcttcc agatcaaagc
      181 cccaggattt tttgacttct ctgaaagatc ccattgcagt agctgcaaat gcttcatgga
      241 tttcgaagtt gtcaatgtca gtgaacttga gccctgcatt tgtgagagcc ttgttcattg
      301 agaatgccat accgattccc atatgagcag gatccactcc gtacattccc cagcctctga
      361 gatatgcctg aattttcagg ccgagttttt tagccttgct ttcagtagtt acaaatattg
      421 cacctgctcc gtcagatctt ggacatgcgt tgaaaagagt cacagctgcc tgagtctttc
      481 cttcttcata ctttttgttg atccactcgc cgtatttctc atagaattct ttcaggctca
      541 taaacttgga atcaaagatc actccagctt tgtcaaatct gcctgggttt tccaccatgc
      601 cgcccttgga cataatgtac tcatcatctt ccagaagaga tccgtcagtc agttttacag
      661 atacctgata cggtctgtac tttcctgcct tgatggaatc gtaagctttt ttataactca
      721 tgtacgcgta gtcatcgagt tcttttttag tcagatcata gttctgagca gcaagttctc
      781 cagtcatcgc catattcagt tttctgatag gatcattcag tccttcatca attgaatcat
      841 ataccttgac acccagtgca ggagcatcag cccagttcgc catcagtttg tctacagtag
      901 ctgtcttgga gttttccttt gcaccttcaa tgatgaaagg cccctgagac attgtttcca
      961 ttccgagtgc cagatagaaa tcaccttcac cgatcataat gcgtcttgct gcatccagaa
     1021 ctgcttcagt tcccgataca cagttgttcg ctacagtgac tgcagatgct tccagaggaa
     1081 ttcctatttt tacagaaatg actcgggcaa tgtttggagc ttttgcactc tggctgattt
     1141 cgccggcaac tacaccgtct acttcagtct tagagacacc tg
//
LOCUS       DAOQGJ010001666         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_99872_length_1182_cov_3.017746, whole genome shotgun sequence.
ACCESSION   DAOQGJ010001666 DAOQGJ010000000
VERSION     DAOQGJ010001666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99872_length_1182_cov_3.017746"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..372
                     /locus_tag="PK683_20530"
     CDS             154..372
                     /locus_tag="PK683_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNH10894.1"
                     /translation="MSSEELLKRITMNPDICFGKPCIRGMRWPVEIVLDLIGSGMSFE
                     EIIADHPELEIQDLLATLKYEETRSFSS"
     gene            406..645
                     /locus_tag="PK683_20535"
     CDS             406..645
                     /locus_tag="PK683_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10895.1"
                     /translation="METLILEISDPKDKEVILLLAERLHCKVVSSEDKSSKKNSAKAF
                     KHLENIAKIGNLKKSIPDPVKWQKEIRKDRKLPGR"
     gene            649..780
                     /locus_tag="PK683_20540"
     CDS             649..780
                     /locus_tag="PK683_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10896.1"
                     /translation="MLLDSNAIIASTALIHKIPLVTANEKDFSWIKDLTIINPLKKK"
     gene            complement(857..>1182)
                     /locus_tag="PK683_20545"
     CDS             complement(857..>1182)
                     /locus_tag="PK683_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10897.1"
                     /translation="KAIHYERLPILTVQAVKEIKSLYDHEKKFREDLSIRLNTTANQL
                     KSATEELASVKTQNTASQKENARLREEIKSLTDSVRTHARVSGQLEERLNALERMQLA
                     TARKK"
BASE COUNT          325 a          253 c          258 g          346 t
ORIGIN      
        1 ggcgaccgaa gggaggcctg ccagggcgag cttgcgagcg ggcggctgtt tcaaatcttt
       61 tctcgcctcg actgcgtctc gcggggctag gggttgaggt acgcccccgt ttttccgctt
      121 ggacagacac tgcttccgca gaattcttca aatatgagct cagaagaact gctgaaacga
      181 attacaatga atcctgacat ttgtttcggg aaaccctgca ttcgggggat gagatggccg
      241 gtcgaaatcg tcctcgattt aatcggttcc ggaatgagtt tcgaagaaat tatagcagac
      301 catcccgaat tggaaataca ggatcttctt gccacattaa agtatgaaga gacaagaagt
      361 ttttcttctt gactgacaag gcagaggttc agtaaactta agtttatgga aacattgatt
      421 cttgaaatat ctgatcccaa agataaagaa gtcattctgc ttcttgctga gcgtctgcac
      481 tgcaaggtag tcagttctga ggataaatcg tccaaaaaaa attctgccaa agcgtttaag
      541 catctggaga atatagcaaa aatcggaaat ctgaagaagt cgattcctga ccctgtaaaa
      601 tggcagaaag agattcgaaa agacagaaaa cttcccggca gataagttat gcttcttgac
      661 agtaatgcca ttattgcttc cacagcactg atccataaga ttccgcttgt tactgcaaat
      721 gaaaaagatt tttcctggat caaagatctt acgattatca atcctctgaa aaagaaatag
      781 tgattgtgtt cattctttga ggttgtgccc aaataataaa agcctgcaaa ccaattagaa
      841 tctctgttat tagatttcat ttcttccttg ccgttgctaa ctgcatcctt tcgagcgcat
      901 tcagtctttc ttccagctgc ccagatacac gggcgtgcgt ccttacggag tctgtcagag
      961 atttgatttc ttcccgcaga cgtgcgtttt ccttttgaga agcagtattc tgagtcttca
     1021 cggaagccag ctcctccgtc gctgatttaa gttgattagc tgtcgtgttc agtcgaatgg
     1081 aaagatcctc tctgaatttt ttctcatgat catataatga cttaatttcc ttaactgcct
     1141 gcacagtcag aattggaagt ctttcatagt gtatcgcttt ca
//
LOCUS       DAOQGJ010001667         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100006_length_1181_cov_4.523091, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001667 DAOQGJ010000000
VERSION     DAOQGJ010001667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100006_length_1181_cov_4.523091"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PK683_20550"
     CDS             complement(<1..262)
                     /locus_tag="PK683_20550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH10898.1"
                     /translation="MSKPIRLILADDHSVVTEGIRAVFRSDSRYEVCGTFSNAESVLK
                     YLEKESADMILLDIDMPGAGNFALLKELKKKYPFIKVVIFTMY"
     gene            504..647
                     /locus_tag="PK683_20555"
     CDS             504..647
                     /locus_tag="PK683_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10899.1"
                     /translation="MKDTLLLIVNEIERFCQIPSGKRIDLIMDVWLLWTCSIERLRNT
                     RVV"
BASE COUNT          339 a          230 c          261 g          351 t
ORIGIN      
        1 cgtacatggt gaatattaca acttttatga aaggatattt tttcttcagc tctttgagaa
       61 gtgcaaaatt tcctgctcca ggcatatcaa tgtccagaag aatcatatcc gcagattctt
      121 tctccagata cttcaggaca gactctgcat ttgaaaaagt cccgcagact tcatatctgg
      181 aatctgatct gaatactgct ctgattcctt ctgtgactac agaatgatcg tctgccagta
      241 tgaggcgaat cggttttgac ataaacctat tctccagcct atgggaaaga tgacaaaaac
      301 aattggattt tactctcgca gaagtttagg ggaagcccta aggtttttgg ggttttgtgc
      361 aaaaaaagtc cctatggacg gtgcagagag gtgtcctgca tttcccacag acaaaaatct
      421 ttcatccgcc atattctttt tctgtccagc tgagtttcgg atttcggttg aatatcagaa
      481 aatgcgattc ttctgcttcg agtatgaagg atacattact tctgattgtc aatgagatag
      541 agcgtttctg ccagattcca tctgggaagc ggatagatct gataatggat gtctggcttc
      601 tttggacctg cagtattgaa agacttcgta atacaagagt tgtctgatct gtcaagactt
      661 tggtgcactt accgattcaa gagggcttcg ccaccgctgt cagtcaccgg tcactgagtt
      721 cgaaatatcg aatgtttcga agtgtccttg cggcaagaag actatgctcc ctcaggctct
      781 ggtcactgag cttgtcgaat gttttgaggg agcaattgaa aaaccttagg gcttccccta
      841 aggtttttcg ggatttgtgc aaaaaaaccg gaattgagac tgaaaaaaat acagaatctg
      901 cttgcagcta cattttgcac actgagaatt gaatgcatta ttctatataa aaagactcgg
      961 tactgtattg tttgctgatt attttttaat agatactagg agaacttatg aaagaaattc
     1021 ggaaaagaat tcagaatgct gcagtatcca cactgatgat cagcttgttt actgtcggat
     1081 gcgataaaaa gaaggatgac aatacaaaga cattggcatt gggcgcagct gcattgagtg
     1141 cgagcagaag tactggaagc acagcttcta cttacacagt c
//
LOCUS       DAOQGJ010001668         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100032_length_1181_cov_3.100355, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001668 DAOQGJ010000000
VERSION     DAOQGJ010001668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100032_length_1181_cov_3.100355"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PK683_20560"
     CDS             <1..878
                     /locus_tag="PK683_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH10900.1"
                     /translation="FAVGTYDEQERYMPKWQSNFLIEFSSPGKIRDNVYRFVIDFGSD
                     IRHSLAYQGLKLGDIDGWYCSHPHADHIGGLEGIALSTVFNPFWNRKKTEWLKNSDGG
                     MDSVNDRLFDGNNVPAECKPMLFSHKSVLAELWEAAAPGLRTLQGVQEVSLDTYFDVK
                     EMKSNENFRFEDGSRHWLVDTVLSTHVLAGTTLMPSFGLRFRSSDGKNILFPTDTLWM
                     MPPNMAGFYKRADIIYQDCETGFKSGVHSHIDDIRMAAPEIKKKCYLYHFAEPPVTDP
                     EEFKGILTQGESHEY"
     gene            complement(880..>1181)
                     /locus_tag="PK683_20565"
     CDS             complement(880..>1181)
                     /locus_tag="PK683_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10901.1"
                     /translation="YKLYLNQETYRYVFRILALKLILSNPENYGYSLSDRDQYKEYNY
                     KKVTLKESVSSLPDFAISNGITYKELIYHNPWIRTGKYSFDPPGNRTYEVLIPEK"
BASE COUNT          321 a          238 c          291 g          331 t
ORIGIN      
        1 cttttgcagt cgggacttac gatgagcagg agcgttatat gccgaaatgg cagtctaatt
       61 ttctgataga attcagttct cccggtaaga ttcgggacaa tgtttacaga ttcgtaatag
      121 atttcggttc agacatccgt cattctctgg cttatcaagg actgaaactc ggagacattg
      181 acggatggta ctgcagtcat ccccatgcag atcatatcgg cggactggag ggaattgcac
      241 tgtctacggt atttaatccc ttctggaaca gaaaaaagac ggagtggctt aagaattcag
      301 acggggggat ggacagtgta aatgacaggc tgttcgacgg gaataatgtg ccagctgaat
      361 gcaagcctat gctcttcagt cataagtcag tgcttgcgga actttgggag gcagcggctc
      421 ccggcctcag gactcttcag ggagttcagg aagtcagcct tgacacctat tttgatgtga
      481 aggaaatgaa gagcaatgag aacttccgat ttgaggacgg aagcaggcac tggcttgtgg
      541 atacagtctt gagcactcat gtactggcag gaacaacttt gatgccttcc ttcggtctgc
      601 gattcagaag ctctgacgga aagaacattc tgtttccaac agacactctc tggatgatgc
      661 ctccgaatat ggctggattt tataagcgtg ctgatattat ttatcaggac tgtgaaacag
      721 gcttcaagtc aggagtgcat tcccatattg atgatatcag gatggccgca cctgaaataa
      781 agaaaaaatg ctatctgtat cattttgcag aacccccagt gacagatcct gaggaattca
      841 aaggaattct gacacaggga gaatctcacg aatactgatt tatttttccg ggatcagcac
      901 ctcgtaggtt ctgtttcctg gaggatcaaa actgtacttg cctgtgcgga tccaaggatt
      961 atggtagatc agttccttgt atgtgattcc gttggagatc gcaaaatccg gaagactgct
     1021 tacagattct ttaagagtga cttttttata attatattcc ttgtactgat ccctgtcaga
     1081 cagactgtac ccgtaatttt ccggattgga gagaatcagc ttcaatgcca gaattctgaa
     1141 gacatatctg tatgtctcct ggttcagata gagtttataa t
//
LOCUS       DAOQGJ010001669         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100172_length_1180_cov_3.699556, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001669 DAOQGJ010000000
VERSION     DAOQGJ010001669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100172_length_1180_cov_3.699556"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1119
                     /locus_tag="PK683_20570"
     CDS             175..1119
                     /locus_tag="PK683_20570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH10902.1"
                     /translation="MFLEVKNLTKTFQTPFSRKPPMKAVQGISFNIRPGEIYALLGPN
                     GAGKSTTIKMIAGLIRPTSGEILLDGKSAVGHSVVHKYLSAILEGNRNVYWRMNPLEN
                     LHYFANLRGVPSSQIEEKAEKILEELNIDAKKKNQSQHLSRGMLQKLALGVGLITDPK
                     LLLLDEPTLGMDVASSRKMRDMILALAKKEGKAILLTTHQLELVEELADRVGIIRNGQ
                     LVVEGTLAELKSIFQTYIYNVKLRGDWNPDEKIIKEYSITSKPRQGEYTEVSADCGSK
                     DGIFRFLDRIRNDSLEVISFYKLEEDLEEIFLRVLKEN"
BASE COUNT          377 a          241 c          258 g          304 t
ORIGIN      
        1 tcttttcagc actctgatat tatgtattta tatgatatac ctttcctgga aactcgtaaa
       61 aaataccgaa gaaaaagccg caaaaagctt cttcttcttc agcatatttc atcttttcct
      121 gattaatttt atcatacttg ctgattttgc agttcagtcc aaaaggatca tctgatgttt
      181 cttgaagtaa aaaacctcac taagacattt caaacaccgt tttcaaggaa gcctccgatg
      241 aaggctgtac agggaatcag tttcaatatc cgtccgggag aaatttacgc actgctcggg
      301 ccgaacggtg cgggtaaatc tacaaccatt aaaatgattg caggactgat ccggcctact
      361 tcaggtgaaa tccttctgga cgggaaatct gcagtcggtc attcagtcgt gcataagtac
      421 ctgagtgcaa ttctggaagg aaacagaaac gtatattgga gaatgaatcc tctcgaaaac
      481 ctgcactatt ttgcaaattt gagaggtgtt ccaagctccc agattgagga gaaagcagaa
      541 aaaattctag aagaactgaa cattgacgca aagaaaaaaa atcagagtca gcatctttcg
      601 agaggaatgc tccagaaact ggcactggga gtaggactca tcactgatcc gaaacttctc
      661 ctcctcgatg aacctactct gggaatggat gtggcaagtt cgaggaaaat gagagatatg
      721 attcttgctc ttgcaaaaaa agaaggcaag gccattcttc tcacaactca tcagctggaa
      781 ctcgtggagg aacttgcaga cagagtcgga atcatccgaa acggtcagct cgtagtggaa
      841 ggaactcttg ctgaactgaa gtccatcttt cagacctata tctacaatgt aaagctcaga
      901 ggtgactgga atccggatga aaaaatcatt aaggaatatt ccattacatc aaagcccaga
      961 cagggagaat ataccgaagt ctctgcagac tgcggaagca aagacgggat cttcagattt
     1021 ctggacagga tcagaaatga cagtctggaa gtgatcagtt tctacaaact ggaagaagac
     1081 ctcgaagaga tttttcttcg agtattaaag gaaaactaga gtccccattt ctgaaggatt
     1141 ccgttgattg tgccgttctc ttccagaatc agaagggtgt
//
LOCUS       DAOQGJ010001670         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100180_length_1180_cov_3.453333, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001670 DAOQGJ010000000
VERSION     DAOQGJ010001670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100180_length_1180_cov_3.453333"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK683_20575"
     CDS             complement(<1..480)
                     /locus_tag="PK683_20575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH10903.1"
                     /translation="MKKPFYTLAILDDHPVMHIAIKNLLKKQDKIRFVKFFSSESDFI
                     SFVEQSPVDMVILDITLNSMNGIEILQLLKKKYSQMKIVIFTMHESPFYFSQAVNSGA
                     DGYILKTDNITVLPEVLGKIADGEKYYSSSLKQFSEQSQEKLTKRESEILNLICSGVS
                     "
BASE COUNT          379 a          220 c          198 g          383 t
ORIGIN      
        1 tgacactccg gaacatatca agttcaagat ttccgattct ctcttagtca gcttttcctg
       61 actctgttcc gaaaactgct tcaaagaaga ggaatagtat ttttctccat cagcgatctt
      121 tcctagaact tcgggaagta ctgtaatatt atctgtcttt aaaatatatc cgtctgctcc
      181 tgaattcacc gcctgggaaa aatagaaggg gctttcatgc attgtaaaaa tcactatctt
      241 catctgactg tatttctttt tcagaagctg aagaatctca attccgttca ttgagttcag
      301 ggttatatca aggatcacca tatcgacagg actctgttca acaaaactga taaaatcgga
      361 ttccgaagaa aaaaatttta caaatcttat cttgtcctgt tttttaagca gattcttaat
      421 tgcaatatgc ataacaggat gatcatccaa gatagccaat gtgtagaaag gtttcttcat
      481 aattgtcccg tacatgtgaa gtatacaaag aatgaattag aaagtctatc attttttata
      541 cttataatct taataactca tcgtaaggtt ctgcttatgc ttcggtcaga cttcattttt
      601 caagtatgct gaaaaacagg aactgactct ttctttagac tgaaagcggg agaaattaga
      661 aacagaatac ctgtaaaaaa ttctaaggcg gagacaaaat gcggaaaaaa tttcttgact
      721 taagtcaaac ttttatcctg tgatttcttt ttcgtttttc tcggatgctg agtaatggcg
      781 cagtaggtaa aaacacctac cactttagtc ataaaaaatc tgcttatcgg gaatatctta
      841 ccggcagtca ggaatggaat gtcctgcata tttttacgga tgaccccgta aaattaccta
      901 attttcaaaa acattttgta gaaagaaaac tgaactccct gaagccttat ccacggatca
      961 ttgtactgat caaaaaaatc gcttattgga caagaaatga ttaaatggat ctttttaatt
     1021 atagtgcttt ttataatagg tattcttagt ctgtcaaact gcagaaacac agacagaata
     1081 tccgcctgca ctgattccaa attatttcct gatctgcctg ctgctgtttt acctaaatca
     1141 ggatcttagg aaaatactga ctggcttgaa aatgtattac
//
LOCUS       DAOQGJ010001671         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100210_length_1180_cov_2.796444, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001671 DAOQGJ010000000
VERSION     DAOQGJ010001671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100210_length_1180_cov_2.796444"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK683_20580"
     CDS             complement(<1..194)
                     /locus_tag="PK683_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002102707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase LigA"
                     /protein_id="HNH10904.1"
                     /translation="MPEGKNMKKENAESEIRKLEEEIRHHQYLYYVKNEPVISDRDFD
                     FLFKKLQKLEEENPKLASPNS"
     gene            complement(187..>1180)
                     /gene="pheS"
                     /locus_tag="PK683_20585"
     CDS             complement(187..>1180)
                     /gene="pheS"
                     /locus_tag="PK683_20585"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNH10905.1"
                     /translation="KSVSRIEGTDADAELEKIRNEVLGKKGTLTSILKQLGGMSAEMK
                     KTAGQKANEFSRELEGILTSKKDEVRKKELYAEMEKEKFDSLRPLRQSEYGTLHPITR
                     IQYEVEDIFVSMGFQIMDGPEIETDYNNFEALNFTDDHPARDMQDTFYTDDGNLLRTH
                     TSAIQVRALRILKPPFRIIAPGRVFRYEEVDASHENTFNQVEGMVVGKDISAANLTYT
                     MNTLLSSIFRRETVVRLRPGYFPFVEPGFELDFQCMVCGGKGCSVCKHSGWVELLPCG
                     LIHPNVLKSAGLDPSVWTGFAFGLGLDRLVMMRYGINDIRYFNSGNLRFLKSFA"
BASE COUNT          294 a          298 c          227 g          361 t
ORIGIN      
        1 gagttcggag aagccagttt cggattctcc tcttccagtt tctgaagttt cttgaacaga
       61 aaatcaaaat cccggtctga aatcacaggt tcatttttta catagtacag gtactgatga
      121 tgcctgatct cttcttccag ttttcttatc tctgattctg cgttttcttt cttcatattc
      181 tttccttcag gcaaatgatt tcaggaatct cagatttccc gaattgaaat atcttatatc
      241 gttaatacca tatctcatca ttacaagtct gtcaagtccc agaccgaatg caaaacctgt
      301 ccatacggaa ggatcaagtc ctgcagattt cagcacattg ggatgaatca gtccgcatgg
      361 caggagttcc acccatccgg aatgcttgca gacggaacag ccctttcccc cgcagaccat
      421 gcactggaag tccagttcaa atcctggctc cacgaatgga aaatatcccg gacgaagccg
      481 cacgactgtc tctcttctga atatgcttga aagaagagtg ttcattgtgt aagtcagatt
      541 ggcggctgaa atatcctttc cgacaaccat gccttccacc tgattgaatg tattttcatg
      601 agaagcatcc acttcctcat atctgaacac tcttccggga gcaatgattc ggaacggagg
      661 tttgagaatt cgcagagctc tcacctgaat tgcagatgta tgggttctga gaagatttcc
      721 gtcgtcggta taaaatgtat cctgcatatc tctggcagga tgatcgtcag taaaattcag
      781 tgcttcaaaa ttattgtagt cagtttcaat ctcagggccg tccataatct gaaatcccat
      841 cgatacaaaa atatcctcca cctcatactg tatccttgta atcggatgga gcgttccgta
      901 ttccgactgg cgcagaggcc ggagagaatc gaatttttcc ttttccattt cagcatagag
      961 ttctttcttt ctgacctcgt ccttcttgga agtcagaata ccttcaagct ccctggaaaa
     1021 ctcatttgct ttctgtcctg cagtcttctt catctctgca ctcataccgc cgagctgctt
     1081 cagtatggaa gtcagtgtgc ctttttttcc aagcacctca ttcctgatct tttcaagctc
     1141 tgcatcagcg tcagtccctt caatccggga cactgacttc
//
LOCUS       DAOQGJ010001672         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100353_length_1179_cov_3.487544, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001672 DAOQGJ010000000
VERSION     DAOQGJ010001672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100353_length_1179_cov_3.487544"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK683_20590"
     CDS             complement(<1..888)
                     /locus_tag="PK683_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HNH10906.1"
                     /translation="MNVSEFNSARVRDVNGKGFNQYGRYILYWMQSSRRFHSNHSLDY
                     AVQLSQKSGRPLVIYEGLGSAYPWNSERIHRFILEGMAENRVFAEKNGITYWSFAESR
                     KNPAGRILRTISEDAYAVITDDNPSFIFPEQTLKLSQKINVQLTAVDSNGIIPLKHYG
                     EPVSAARVLRPRLHRLFAEGYMNRAVREHSWKKILFLKEKAPFTEFTAGKGAVESVLS
                     DIDFSYSVGPAASVSGGRNAGLGRLNDFIIKKLSKYSERSNPYPPELSSASGLSPYVH
                     FGLLSADEIAEAVLNAASED"
     gene            complement(885..>1179)
                     /locus_tag="PK683_20595"
     CDS             complement(885..>1179)
                     /locus_tag="PK683_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10907.1"
                     /translation="GAFQTLEENRAELRRINSGLRDITSKINNGKGTVSSLINRDTLY
                     DNMNESMTDLSVISREARWYMEMSRENDTVPFPFLSTVTLNLLSLNYLSQSTK"
BASE COUNT          313 a          279 c          256 g          331 t
ORIGIN      
        1 gtcttcggat gcagcattca gaactgcttc tgcgatttca tctgcagaca gaagaccgaa
       61 atgaacataa ggcgacagac cgctcgctga ggaaagttca ggcggatacg gattggatct
      121 ttcagaatac tttgaaagct ttttaatgat aaaatcattc agcctgccga gtcctgcatt
      181 tcttcctccc gaaacggaag cagccggacc gacactgtat gaaaaatcaa tatcagagag
      241 aacagattca actgcgccct ttcctgcagt aaactcagta aacggtgctt tctccttcag
      301 aaaaagaatc tttttccagg aatgctccct gacggccctg ttcatataac cttccgcaaa
      361 aagtctgtga agccgaggcc tgagaaccct tgctgcagat acgggttccc cgtaatgctt
      421 cagaggaatt attccgtttg aatccactgc agtgagctgt acattgattt tctgcgagag
      481 tttcagtgtc tgctcgggaa agataaagga aggattgtca tccgtaatga cggcatatgc
      541 atcctctgaa attgtccgga gaatcctccc ggcaggattt ttccttgact ctgcaaaact
      601 ccagtatgta attccgtttt tttccgcaaa gactctgttc tcggccatgc cttcgagaat
      661 gaaccgatga atcctttcgg aattccaagg ataggcagat ccaagtccct cgtaaatgac
      721 aagaggcctc cctgacttct gagaaagctg aacggcataa tccagtgaat gattgctgtg
      781 aaatcttctt gaagactgca tccagtacag tatgtatctt ccgtactgat tgaagccttt
      841 tccgtttaca tctctgactc tcgcagaatt aaactccgat acattcattt tgtgctctga
      901 ctcagatagt tcagcgaaag aagattcaag gtgacagtgg aaaggaaagg aaacggcaca
      961 gtatcatttt ccctcgacat ttccatgtac cagcgtgctt ccctggaaat gaccgaaagg
     1021 tctgtcatag actcgttcat attgtcatac agtgtatccc tgttgatgag actagagacg
     1081 gttccctttc cattgttaat tttggaggta atgtcgcgca gtcctgaatt tatccttctg
     1141 agttccgccc tgttttcttc gagagtctga aatgctccg
//
LOCUS       DAOQGJ010001673         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100354_length_1179_cov_3.460854, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001673 DAOQGJ010000000
VERSION     DAOQGJ010001673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100354_length_1179_cov_3.460854"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PK683_20600"
     CDS             complement(<1..1161)
                     /locus_tag="PK683_20600"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH10908.1"
                     /translation="MAEKPEQVFHEEHQVLEEFRNALKSDLTIVSLKHQIDKFTGAYE
                     SLLKQSEKLMKLGDSAQRRLMKTQEDLKSSKSELEDSYKSLNGISQIGQAITSSMDVK
                     NIVETVFNYLYYSNSKPDFIAIGIYDENGWIKYKYSYKNGQFIPALTIDALNEENFSK
                     LCVEKRWSVITGKNNSEHSVQLENWKMIWGEVYESMLFMPLQIEKRIIGILTVQSSTE
                     KIFTDNYLSILSTLTSYLGIALDNADAYKSLARRNKQLKETLHKINQLNANLEMEREK
                     SDRLLLNILPASIAERLKAGESVIADYFESSTILFADIAGFTKLSAQMESPAKLVEIL
                     NSIFTAYDSIASSYQLEKIKTIGDCYMLAGGIPTSTEDHPEKIVRAGLDMIKI"
BASE COUNT          317 a          237 c          229 g          396 t
ORIGIN      
        1 aattttgatc atatccagtc ctgcccttac aatcttctca ggatgatcct cagtggatgt
       61 agggattccg cctgcaagca tataacagtc gcctattgtt ttaattttct ccaactgata
      121 ggaggatgca atggaatcat aggcagtaaa aatagaattc agaatctcta ccagttttgc
      181 gggagattcc atctgtgctg agagtttagt gaaccctgca atgtctgcaa aaaggattgt
      241 ggaggattca aaataatctg caatgacaga ttctcctgcc ttgagtcttt cggcgatgct
      301 ggcaggaaga atattcagaa gaagcctgtc ggatttctct ctttccattt cgagatttgc
      361 attcagctga ttgatcttat gaagagtttc tttgagctgc ttgtttctcc tcgccagact
      421 tttgtaggca tctgcattgt ccaatgcaat ccctaaatag gaagtcagtg tagaaagaat
      481 gcttaaataa ttatcagtaa atattttctc tgtagaactc tgtactgtaa gtatcccaat
      541 gattcttttt tcaatctgaa gaggcataaa gagcatagac tcgtacactt caccccagat
      601 cattttccag ttttccagct gaacggaatg ttctgaattg ttttttccag tgatgacact
      661 ccagcgtttt tcaacacaga gtttggaaaa attttcttca ttgagtgcat cgattgtcag
      721 tgcaggaata aactgtccgt ttttataaga gtacttatat tttatccagc cgttttcatc
      781 gtaaatgccg attgcaataa aatcaggttt gctgttggaa tagtatagat aattaaatac
      841 cgtttctaca atatttttta catccatact ggatgtaatc gcctgtccaa tctgactgat
      901 gccgttcaga cttttgtagg aatcttccag ttctgattta gaacttttca gatcctcctg
      961 tgttttcatc agccttcttt gggcagagtc accaagcttc atcagctttt cggactgttt
     1021 caggagactt tcataagctc cagtgaattt atctatctgg tgcttcagag aaacaatggt
     1081 cagatctgac tttaaagcat tcctgaattc ttcaagaacc tggtgctctt catgaaagac
     1141 ctgctccggt ttctcagcca tttaatgtat cagagaggg
//
LOCUS       DAOQGJ010001674         1178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100562_length_1178_cov_2.516474, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001674 DAOQGJ010000000
VERSION     DAOQGJ010001674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100562_length_1178_cov_2.516474"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..>1178)
                     /locus_tag="PK683_20605"
     CDS             complement(415..>1178)
                     /locus_tag="PK683_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10909.1"
                     /translation="IIRNSYTFIMKIKFSDYAGVCICFLFTAAGIWIWFYPGAFLFGF
                     GFIVLSISLYTKKKAYKNLSTEFASIEIEGGRKFTQSPLIFYSTSVGLFLAGSGTMFF
                     WDQKIGLLICGAVAAAFGAGLVLSKLLGLFGNQFISFEETGLLVGRNGYSFLIHWENI
                     AEIRMDEWNNVPYVFLEIISYENAEDTIESADREKSALKLRKSCIWNQGYFGSSFAFS
                     TVQFGMDTALFFKAIIQYLERPEKREELALRKRIE"
BASE COUNT          352 a          270 c          230 g          326 t
ORIGIN      
        1 tctccatgat cctctgcgga atcatgcttt ccagaagaaa tttacgcctg gatcattttt
       61 gaacccggat ataaagaatc agtaaagaat gattttactt ttccacagca gacctgcctg
      121 ggaaatcaaa gaagacctgt catagtgagc ttgtccgtga gtaaaagggc ggcttgtctg
      181 ctcagtgcag agagctgaag gagtgacaga gtgttcattc atccagaccc ggcagaaaat
      241 atctgcatgc attccgggac attctgactg tatgcaaatt tatttaaaat aaatatatat
      301 atattatata atgtatctgt acccgctctg cttcgctgag tatgctattc actcacctta
      361 cgcctgacgg cggggcgttt gtggcagaga gcctgccggt ttgcgtaacg tgagttattc
      421 aattcttttt ctcaacgcaa gctcttctct tttctccggt ctttcaaggt actgaatgat
      481 cgccttgaaa aacagagcag tgtccattcc gaactgtacc gtggaaaatg caaaactgct
      541 tccgaaataa ccctgattcc agatgcagga ctttctgagt ttcaaagctg acttttccct
      601 gtctgcagat tcaattgtat cttccgcatt ttcataagag atgatttcca gaaatacata
      661 cggaacattg ttccattcat ccatacggat ttctgcaata ttttcccagt ggatcaggaa
      721 actgtacccg ttcctcccaa ccagaagtcc agtctcttca aatgaaataa actggtttcc
      781 gaaaagtcca aggagtttcg aaagaacgag ccctgcgccg aatgccgctg caactgcacc
      841 gcagatcaga agaccgattt tctgatccca gaaaaacatc gttccgctcc ccgcaagaaa
      901 cagcccgacc gaagtggaat aaaaaatcaa aggagactga gtgaactttc ttcccccttc
      961 aatttcaatg gatgcaaatt cagtactgag atttttatat gcctttttct tggtatacag
     1021 actgatcgaa agcacaatga atccgaatcc gaatagaaat gctccaggat aaaaccatat
     1081 ccaaattcct gcagcagtaa atagaaaaca gatacagaca ccggcataat ctgagaactt
     1141 gatcttcata atgaaagtat aactgtttcg aatgattt
//
LOCUS       DAOQGJ010001675         1178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100590_length_1178_cov_2.186109, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001675 DAOQGJ010000000
VERSION     DAOQGJ010001675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100590_length_1178_cov_2.186109"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PK683_20610"
     CDS             complement(<1..1162)
                     /locus_tag="PK683_20610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH10910.1"
                     /translation="MAYTIGYALGGALITALILTPGLAFLAYRRPQKIFHNVWLERVT
                     EAYQKRLEKILESPKKVYKFLAFALVLCIVLTAFIGKDYLPELDEGSIWLQVQLPPGI
                     SLQKGSEMADDLRKETLKYEEVSYAVTQVGRNDDGTDSWTPSHIEASIGLKPYGEWKN
                     GRTKKDLINDLREHYERIPGYIVSFSQPMIDNVNDKISGAHSELVIKVYGDEFRQTRE
                     LTKQVMTVIRNTPGSADVAIDQEPPLPQIQVKIDREASARYSLNAADISDLVETAIGG
                     EAVSNLFLGERKYDITVRYGEKYRNSPEAIGNLTLTTPTGAKIPVSQVADVKLTTGES
                     TITRESNRRHLTVKVNLNGRDLSSFIREAKDNLESQISYDHQKYQIVWGGQLE"
BASE COUNT          284 a          289 c          245 g          360 t
ORIGIN      
        1 tctccagctg ccctccccat acgatctgat atttctgatg gtcataactg atctgagatt
       61 ccagattgtc ctttgcctct ctgataaatg aagaaagatc tcttccgttc agattgacct
      121 taactgtaag atgtcttctg ttggactccc tcgtaatcgt gctctcacct gttgtcagct
      181 tgacatctgc cacctgagag accgggattt ttgctcctgt cggagtagtc agagtcagat
      241 tgccgatagc ttccggtgaa tttctgtact tttcgccgta tcttactgta atgtcatatt
      301 tcctctctcc aagaaagaga ttggaaactg cctctcctcc gattgcagtc tcgacaagat
      361 cggaaatgtc cgccgcattc agactgtaac gagctgaagc ctctctgtct atcttgacct
      421 gaatctgagg aagaggcggt tcctgatcga ttgcaacgtc tgctgaacca ggcgtattcc
      481 gaatgactgt catgacctgc ttagtgagct ctctcgtctg ccggaactcg tctccgtaaa
      541 ccttgatgac aagctcactg tgggctccgg atattttatc attaacatta tctatcattg
      601 gctgagaaaa acttacaata tatcccggta ttctttcata atgctctctg agatcattaa
      661 tcagatcttt ttttgtccga ccgttcttcc actctccgta aggcttgagt ccgatggacg
      721 cttctatatg cgaaggtgtc caggaatcag ttccgtcatc gtttcttcct acctgagtga
      781 ctgcatagga gacctcttca tatttcaatg tctctttccg cagatcatct gccatttcag
      841 agcccttttg aagtgaaatt ccgggaggga gctgcacctg cagccagatt gacccttcat
      901 ccagttcagg aagatagtct tttcctataa aagccgtcag aacaatgcag agaaccaatg
      961 caaaggcaag aaatttataa acttttttag gactttcaag aatcttctca agtcttttct
     1021 gatatgcttc tgtaactctt tcgagccaga cattatgaaa aatcttctga ggtcttctgt
     1081 aggcaaggaa agccagtccg ggagtaagga tcagtgccgt aatcagtgcc ccgccgagag
     1141 catacccgat tgtatatgcc attggggtga aaagcttt
//
LOCUS       DAOQGJ010001676         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100693_length_1177_cov_3.118538, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001676 DAOQGJ010000000
VERSION     DAOQGJ010001676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100693_length_1177_cov_3.118538"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..>1177)
                     /locus_tag="PK683_20615"
     CDS             complement(76..>1177)
                     /locus_tag="PK683_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNH10911.1"
                     /translation="EPDRVYKYKIFTSLAKDGKNLMDNEETAPEVQGYAKSGSTPSAA
                     VPAKVSGLTAEPDFSYDTNSMQMTLVWNKMHNTAKYEIARFSGNKLNIIGSSKENKFI
                     DKSPGNLSEAKYSVRALNSNGSGDWSDAVFIPSYEEEGLIAPSGLKASSNLKDKIELK
                     WNPVKGASYYTVKRTHSKSKEKKEFRVESSLYTDSDTVPGRIYHYSVSAGKGSEKTKY
                     SDNVTGRTVKTQTEKEEKNEFDDFVNSILSIIRGSDSKAQSKFSKMLSLKKEETLAEF
                     LSGNWSGKQWTGGKNSQSFQIKITMDEKKFDAEFIQGKTVRKISGTVIKGADFIEADG
                     FKMQKVFDQTAEVTIHSNKIYPTSLELNFTKD"
BASE COUNT          303 a          248 c          198 g          428 t
ORIGIN      
        1 ccggatttta atcagttctg attccgacag aatgacgcct caaacggaaa gcgcaagttc
       61 caagaatcag cagtatcagt ctttcgtaaa gttcagttcc agcgaagtgg gatatatttt
      121 atttgaatgt attgttactt ctgctgtttg gtcaaatacc ttctgcattt taaaaccatc
      181 agcttcaata aaatccgcac ccttgatcac tgttccgctt attttcctga ccgtttttcc
      241 ctgaatgaac tctgcatcaa atttcttttc atccattgta atttttatct gaaagctctg
      301 tgaatttttt ccgcctgtcc attgttttcc tgaccagttc ccggaaagaa attccgcaag
      361 agtttcctct ttcttcaggc tcagcatttt ggaaaactta gactgcgctt ttgaatctga
      421 acctctgatt atagaaagaa tggaattgac aaaatcatcg aattcattct tttcctcctt
      481 ttcagtttgt gtttttactg ttcttcctgt cacattgtca gaatattttg ttttttcgga
      541 accctttcct gcagatacag aataatgata aattcttcca ggaacagtgt cagaatcagt
      601 ataaagagaa ctttccactc taaattcctt tttttctttg gattttgaat gagttctttt
      661 cactgtatag taggaagcac cctttacagg gttccacttc aattctattt tatccttcag
      721 attcgaagaa gcttttaatc ctgaaggagc aattagtcct tcttcctcat aggaaggtat
      781 aaagactgca tcactccaat cgccagaccc attcgaattc aatgcccgaa cggaatattt
      841 tgcttctgac agatttcctg gagatttatc aatgaacttg ttttctttcg atgaaccgat
      901 aatgttcaat ttatttcctg aaaaacgtgc aatttcatat tttgcagtat tatgcatttt
      961 attccatact agagtcatct gcattgaatt ggtatcgtag gaaaagtccg gttctgcagt
     1021 cagaccgctt accttggctg gaactgctgc agaaggagtg cttcctgatt ttgcataacc
     1081 ctgtacttca ggtgcagtct cttcattgtc catcagattt tttccgtctt tggctaaaga
     1141 ggtgaatatt ttatatttat atactctgtc tggctct
//
LOCUS       DAOQGJ010001677         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100703_length_1177_cov_2.945633, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001677 DAOQGJ010000000
VERSION     DAOQGJ010001677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100703_length_1177_cov_2.945633"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1177)
                     /locus_tag="PK683_20620"
     CDS             complement(<1..>1177)
                     /locus_tag="PK683_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10912.1"
                     /translation="RYLSKYLKQITSEELNRMFYQFRICLLLQTGRDMGNESFTESEE
                     KFEAFLDSWKLKNANEFFALIRKYIFNNYNDYIINYIKNIKISESENSVLKNPVENLL
                     ADSESPDRDSTVIDGKAVENINSISSEKKPEDDYTLMHNFEKIETADEFNGMMRDMDG
                     EDDLKNHSEALKDLNLKDTVRSDEKAHSIYRTDLTMNSTVGFAGDSEKSKGIYYSEWD
                     FSRKQYKEKFCTVYPRHPEKSDHNYVLKAESDLKNSYEKLEKLFHKQLRETESLKRQK
                     SGSEVDLDAVVDNYTFAKAGCSASEMLYVNRKPARLDLALLILIDISLSTDSYVQNQR
                     IIDVEKKSTALFAKLLDRYKVHFQIDAFSSRTRNNCEYIHIKTFSEKWKSRKHFLGGI
                     "
BASE COUNT          295 a          247 c          217 g          418 t
ORIGIN      
        1 aaatccctcc caggaaatgt tttctggatt tccatttttc tgaaaatgtc ttgatgtgaa
       61 tgtattcaca gttgtttctt gtgcgggagg aaaatgcatc aatctgaaaa tgaactttgt
      121 atctgtccag aagttttgca aacagtgcag tcgatttttt ttccacatca atgatcctct
      181 gattctgcac ataggagtcg gtggaaagac tgatgtcgat cagaatgaga agggccagat
      241 ccagcctggc aggttttctg ttcacataca gcatctcgct tgcactgcat cctgccttcg
      301 caaaagtata attgtccaca actgcatcca gatccacttc cgatccggac ttctgtcttt
      361 tcagggattc cgtttcacga agctgcttat gaaagagttt ttcaagtttt tcataggaat
      421 ttttcagatc ggattctgct ttcaggacat agttatgatc tgatttttca ggatgccttg
      481 gataaactgt acagaacttt tccttatact gttttctgga aaaatcccat tccgaataat
      541 aaattccttt acttttttcg gaatctcccg caaaccctac agtggaattc attgtgagat
      601 ccgttctgta tatggaatga gccttttcat cggatcttac tgtatccttc agattcagat
      661 ccttcagtgc ctctgagtga tttttcaggt catcctctcc gtccatatcc ctcatcattc
      721 cgttgaactc gtctgccgtt tctatttttt caaaattatg catcagtgta tagtcgtcct
      781 ccggtttttt ttcagaggag atggaattga tgttttcaac tgcctttccg tcaatcacag
      841 tagagtctcg gtccggtgat tctgagtcgg caagcaggtt ttccactgga tttttcagta
      901 ctgagttttc agactcggaa attttaatat ttttaatata atttattata tagtcattat
      961 aattattgaa tatgtatttc cggatcaggg caaagaattc attcgcattt ttcagtttcc
     1021 atgaatccag gaaagcctca aatttttctt ccgattctgt aaaggattca tttcccatgt
     1081 ctctgcctgt ctgaagaagc agacagattc tgaactgata gaacattctg ttcagttcct
     1141 cactggtgat ctgctttaaa tactttgaca aataccg
//
LOCUS       DAOQGJ010001678         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100821_length_1176_cov_3.914362, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001678 DAOQGJ010000000
VERSION     DAOQGJ010001678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100821_length_1176_cov_3.914362"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PK683_20625"
     CDS             complement(<1..247)
                     /locus_tag="PK683_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10913.1"
                     /translation="MNTVKTAFIFLFIAFFPLFSKEKPMEMIYKDAVVYTGYTKYIFT
                     DPKTKKDWELDVPSDEVKKYSIPKTMLENPEEVEGLVG"
     gene            complement(420..1001)
                     /locus_tag="PK683_20630"
     CDS             complement(420..1001)
                     /locus_tag="PK683_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10914.1"
                     /translation="MNFPVKLKFKIFAIAPQIYAYDSTGHLVHYIKQKLFKLKEEVTV
                     FADEDQEKPQYKIAADRIIDFSAAYSFTDMQGRKLGSIKRKGMKSLWSAHYEIYDAEG
                     EKLFTVEEENAWIKVIDALFGEIPVVNIFMGYFFNPKYSVSSGSGMEYRITKKRSFLE
                     SGFEVSKEKQGASNETLVLLGAMMFVLLERMRG"
BASE COUNT          359 a          253 c          199 g          365 t
ORIGIN      
        1 caccgacaag tccttccact tcctccgggt tttcgagcat agttttcgga atggaatatt
       61 ttttcacctc gtcactcggc acatccagct cccagtcctt ctttgttttg ggatcagtga
      121 agatatattt tgtatatcct gtgtatacaa cggcatcctt gtagatcatt tccataggct
      181 tttccttgga aaaaagagga aagaatgcaa tgaagagaaa aataaaagct gttttaacag
      241 tgttcataat tgtaagttcc ttataatata tttcctgctc agcagccgca gaaacaaagc
      301 ggaaaccgcc ggaggaattc gctgcaagcc tgaaatcttg ccggtctgga agtcggctcg
      361 acgattcgga agcttactga aattcccatt ttctgaaaac aagtataatt attcaaaaat
      421 tatcctctca ttctttcgag aagaacaaac atcatagctc ccagaagaac aagtgtttca
      481 ttggatgcac cctgtttttc cttcgaaact tcaaaaccgc tttccagaaa cgatcttttc
      541 tttgtgattc tgtactccat tccagaacct gaggaaacag aatacttcgg attgaaaaaa
      601 tatcccataa aaatattgac cacagggatt tctccgaaaa gtgcatcaat cactttgatc
      661 caggcattct cctcttccac tgtgaaaagt ttttcacctt ctgcatcata gatctcataa
      721 tgggcagacc agagagattt cattcccttt ctttttatgg aacccagttt tcttccctgc
      781 atatccgtaa agctgtatgc tgctgaaaaa tctatgatcc tgtctgctgc aattttgtac
      841 tgcggctttt cctgatcctc gtctgcaaat acagtcactt cttctttcag tttgaacagt
      901 ttctgcttaa tataatggac aagatgtcca gtggaatcat aagcatatat ctgcggtgca
      961 atcgcaaaaa ttttaaattt cagttttaca ggaaagttca taacccctct tcagtattgt
     1021 tgggataata taaaaaatga tgaaaataaa gaaagaaaaa aattttccca aaaacagaac
     1081 agcagtccat tcaaatgaaa attcttcacc ccggatccgg aggaaaggat ttttccgtat
     1141 ctatgcagta tttactatat cattatatag taatca
//
LOCUS       DAOQGJ010001679         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100834_length_1176_cov_3.285459, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001679 DAOQGJ010000000
VERSION     DAOQGJ010001679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100834_length_1176_cov_3.285459"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..344
                     /locus_tag="PK683_20635"
     CDS             204..344
                     /locus_tag="PK683_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10915.1"
                     /translation="MKTDRALKSWIENDLKTKMVFLGGPRQVGKTTLAKSFTERADMF
                     LI"
     gene            complement(382..>1176)
                     /locus_tag="PK683_20640"
     CDS             complement(382..>1176)
                     /locus_tag="PK683_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10916.1"
                     /translation="KVQAVKLDISWSKASDNVSSQADLEYKIMMSSSNNISDVTGMET
                     SGSGRVLIMDWTKNVSTYTVTGLSVNNTYYFNIIVKDQPGNKSAYSSGSSLTKDVYDN
                     FNGTVSSYSRNRTYTKCSMGQVWNSPENDCNGTGSGGDGYGYTSHQYCNTANDSCNPT
                     GPSWDLDGRGNSGVWTVCDQLVLAGKTDWRVMNSGELFEFYQSIYSVNLSFFPRMIMA
                     FYFASSANSGFMQNGFASAVNMQFGTQSNLKSKTDSSPMLCVRDGL"
BASE COUNT          304 a          281 c          233 g          358 t
ORIGIN      
        1 gcttattaca gcgggcagca ctaaaatcaa ccatttcatt cgtttcacct gtccttcatt
       61 attaactaaa gtgcatcttc cttttcattt tttctgcttt ttttcccggc tcgaaatttt
      121 ttttcaatcc ccaatccata ttcccaagga gcagcttccg ggtctgtgag cgggtgttaa
      181 acatgcctca atttttcagt aatatgaaaa ctgaccgtgc actgaaatcc tggattgaaa
      241 atgatctgaa gaccaaaatg gtgtttttag gcggaccgag gcaggtaggc aaaactacac
      301 tcgcaaagtc ttttacagag agagcagata tgtttttaat ctgaatgccg gagactgtct
      361 tctgaactca tccgcctagc ctcataaccc gtctctcaca cagagcatcg gtgaggaatc
      421 tgtcttggat ttcagattgc tttgtgtgcc gaactgcata tttactgcag atgcaaatcc
      481 attctgcata aaacccgaat tggcagagct ggcgaaataa aacgccataa tcattcttgg
      541 gaaaaatgat aagttgactg aatatatgct ctgataaaac tcaaacagtt ctcctgagtt
      601 catcactctc cagtctgtct ttcccgcaag aacaagctga tcacagacag tccatacacc
      661 gctgttcccg cgaccgtcca aatcccagct tggcccggta ggattgcagc tgtcattcgc
      721 cgtattgcag tactgatgac ttgtataacc gtatccgtct cctccgcttc cagtgccgtt
      781 gcagtcgttt tccggagaat tccagacctg ccccatggaa cacttggtat atgtcctgtt
      841 cctggaatag gaagaaactg ttccgttaaa attgtcataa acatcctttg tgagagaaga
      901 tccggaagag tatgctgact tgtttcctgg ctgatctttt acgattatgt taaaataata
      961 tgtgttgttt acagacagtc cagtgactgt atatgtgctt acattctttg tccagtccat
     1021 aatcaggact cgtccgcttc cggatgtttc cattccagtc acatccgaaa tattgtttga
     1081 tgaggacatc ataattttat actcaaggtc tgcctgagaa ctgacattat cggaagcctt
     1141 gctccagctg atgtccagtt tgactgcctg cacctt
//
LOCUS       DAOQGJ010001680         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100854_length_1176_cov_2.994648, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001680 DAOQGJ010000000
VERSION     DAOQGJ010001680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100854_length_1176_cov_2.994648"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1039
                     /gene="dapA"
                     /locus_tag="PK683_20645"
     CDS             155..1039
                     /gene="dapA"
                     /locus_tag="PK683_20645"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020764698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNH10917.1"
                     /translation="MFQGVYTAVITPFSDGKIDYDSYFRILDRQIQAKVSGVVPCGTT
                     GESPTLSHDEHKELIKKTVDYIKGRIQVVAGTGSNSTKEACELTESACKDGADGVLSV
                     NPYYNKPTQEGLYLHFKSIAEVSAKPIMLYNIPGRTGVNLAPETTKRLSMLDRIVSIK
                     EATGDLGQMARTIAILPKNFTLLSGDDNLILPVLSIGGKGVVSVVSNLFPASLVKIVN
                     SFASGKLDEARELYYELLPVFTLAFLETNPIPVKAAMHHLGYCSGEVRLPLTPLSSSK
                     QSEEFLALVKKLKEKGLE"
BASE COUNT          340 a          251 c          265 g          320 t
ORIGIN      
        1 aatttctttt tgattctaat ttgacccaaa tggaattcgc ttcctttata aatctatggc
       61 ttgtgtgtat ttcgtcagaa aatgcggatg atcgatttat ccgttcagtt gaattttaat
      121 tgtaatttta gagtctattt tcgaacctga aaccatgttt cagggagttt acacagcagt
      181 catcactccg ttttcggacg gaaaaatcga ttatgattcc tatttcagga tattggacag
      241 acagattcag gcaaaagtca gcggagtggt tccctgcgga acaacaggcg agtctcccac
      301 tctcagccac gacgagcata aggaactgat caagaagact gtcgattata tcaaaggcag
      361 aatacaggtt gtagcaggaa ccggttccaa ttccacaaag gaagcgtgtg aactcacaga
      421 aagtgcctgc aaagacggcg cagacggtgt tttgtccgtc aatccttatt ataataaacc
      481 aactcaggaa gggctgtacc ttcattttaa gagcattgca gaagtttctg caaaaccaat
      541 tatgctctac aatattcccg gcaggacagg ggtcaatctg gcaccggaaa ccaccaaaag
      601 gctttctatg cttgacagga ttgtttccat caaggaagcc acaggagact taggtcagat
      661 ggcaagaacc attgcaattc tccctaaaaa cttcactctt ctttcaggag acgacaatct
      721 gatccttcct gttctctcca taggaggaaa gggagttgtc tcggtggttt cgaatctatt
      781 ccctgcatct ctggtaaaaa tcgtaaattc cttcgcctcg ggaaaacttg atgaggcaag
      841 agaactttat tatgaacttc ttcctgtatt cactctggca tttttggaga caaatccgat
      901 tcctgtgaag gctgcgatgc atcatctggg atactgttcc ggagaagtga ggcttcctct
      961 gactccgctc agctcatcaa aacagtcgga agaatttctg gcactcgtaa aaaaactgaa
     1021 ggagaaagga cttgaataag agaaacaaag tatgtctcat cggggcaggc ggaagaatgg
     1081 gaaaggcgat cactgaggtg ctttttcatt ccaaaacttc agttctcggt tcagcagtgg
     1141 agaagacgga ttcaatcctt cttggcatgg acagcg
//
LOCUS       DAOQGJ010001681         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_100867_length_1176_cov_2.811775, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001681 DAOQGJ010000000
VERSION     DAOQGJ010001681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100867_length_1176_cov_2.811775"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PK683_20650"
     CDS             <1..384
                     /locus_tag="PK683_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10918.1"
                     /translation="IILSVSVSAKPAVKEGKHSLTLQWIEGKGSINFVKKGKSEFEVS
                     GEQKSSKTEDFLKMEGVIQVRSSTELEFTGKIETQVSYINDGKVCVRDGKHTFKAYGK
                     RKYWRMQDKINCDGKVTDYIDIYFQ"
     gene            526..1044
                     /locus_tag="PK683_20655"
     CDS             526..1044
                     /locus_tag="PK683_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10919.1"
                     /translation="MNLSGNKHTYRTNRILLLIFFSAVSFFFYYMFTYYTYAGRFLET
                     CYSFFHIQKIFNLSPLDLNFFTKIARIFFALYLGFYYVLFFGRVLEYFFSSLVIYPEN
                     KSVYYIHSNGFFRNMKYLHSESKNLEYCDNLLLRIFRTGHIRIHTADSEVWSIGYIYK
                     AGQAFKEILSWV"
BASE COUNT          350 a          201 c          256 g          369 t
ORIGIN      
        1 attattttga gcgtttctgt ctctgccaaa cccgccgtaa aggaaggaaa acattctctg
       61 actctgcagt ggattgaggg caagggttcc attaatttcg taaagaaagg aaaatccgaa
      121 tttgaagtga gcggagagca aaagtcttcc aagacggaag actttctgaa aatggaaggg
      181 gtcattcaag tcagatcctc cactgagctg gaatttacag ggaaaattga aacacaggtt
      241 tcctatatca atgacggaaa agtatgcgtg cgtgacggga aacatacttt caaggcatac
      301 ggaaagagaa aatactggag aatgcaggat aagatcaact gcgacggaaa agtgacagat
      361 tatattgata tatattttca atagctgctt gaccggcccg gataaaaaaa tgaatcagtt
      421 tagactgctg tctctcttaa gtgatgataa gatacgcaag tatatgccgg tgcctgaaat
      481 gaaggatctg cggtttggga agagaagtca ggagggagat cagagatgaa tttatccggg
      541 aataagcata catacaggac gaacagaatt cttctcctga tctttttctc agcagtttct
      601 ttttttttct attatatgtt tacttattac acgtatgcag gaagattttt ggaaacctgc
      661 tattcttttt ttcatattca gaagatattc aatctttctc cccttgatct gaattttttt
      721 acaaaaattg caagaatctt tttcgcactc tatctcggat tttattatgt tctctttttc
      781 ggcagggtgc tggaatattt tttttcgagt ctggttattt atcctgaaaa taagtctgta
      841 tattatattc attcaaatgg atttttccga aatatgaaat atcttcattc tgagtcaaag
      901 aatctggaat actgtgacaa tctgcttctc aggattttca gaacgggtca tatcagaatt
      961 catactgcag attctgaggt ctggagcata ggatacattt acaaagcagg gcaggcattc
     1021 aaagaaattc tcagctgggt gtaaggaatt gcttcatcct gttttagtcg gaatcggact
     1081 tgcacttttt tacggagtgc tgctgtttgc aaatcttcag agatttctgg atcacgatca
     1141 ggccgtttac gcaatgaatg tatggtcctg caacgg
//
LOCUS       DAOQGJ010001682         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101014_length_1175_cov_3.258036, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001682 DAOQGJ010000000
VERSION     DAOQGJ010001682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101014_length_1175_cov_3.258036"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            684..>1175
                     /locus_tag="PK683_20660"
     CDS             684..>1175
                     /locus_tag="PK683_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10920.1"
                     /translation="MNKALRYLSDTYAPKGTSTDGQNEKLKHKFLLSLAVLMSFGGII
                     WGSIALYFGLYLQALIPFGYTVLSAVNISAFLLFRNFSMTRLIQVFISLMLPFLFQWS
                     LGGFISSGAVMLWAALAIIGSLTFGNAKSSITWLILYTLLTVFSGIIDGSLPGFKESG
                     SDSL"
BASE COUNT          372 a          230 c          202 g          371 t
ORIGIN      
        1 ccaaacggaa cagattgcac tgaaattaaa catgataaaa tttcaaacgt tttccgaagt
       61 ctgttctgaa tcagaaaaaa ctgtcaaatt taatactcca gcctcattca tctgcttgat
      121 ttcccattcc gtccaatctt ggaaacagaa cactgcaatt cagatttcga gatttctgga
      181 atacttctaa aagaggagat ttctgaattt tctcaatttt ttttgaaatc aaaaaacagg
      241 cagaatgcat aaatctcaat gcaaacagaa ggaaccgaaa tgaatcaaga ctatttgctt
      301 caaaattcag catgtctcat aattagacaa tattatactt atactattta aataccgtat
      361 aattaaatat atattttata tacaatatat atttttaata tattataaaa atattaaaat
      421 aggtgttact acctattttg ctgcccctgc attccctgta tgcctctttt tctgaaccca
      481 ggacatctcc gaatgaatcg gaacactgag ccgtaccggg ctgttaaaaa taaaaaaacc
      541 agcactgtct atttttggga cgcaggaaat aaaaaatctt gaaacagaga atcatatcag
      601 gaatataaac agagaaaaac tgcacattca gattaaatat gcggacatat gttcatcata
      661 ttttcagaat tataaggaat tttatgaata aagctctcag atacttatcg gacacttatg
      721 caccgaaagg cacgagcact gacgggcaga atgaaaaact gaagcataaa ttcctgctct
      781 cgctcgcggt tctgatgagt ttcgggggca ttatctgggg atcaattgca ttgtatttcg
      841 gtctttacct gcaggctttg attcctttcg gatatacagt tctgtctgca gtcaatattt
      901 ctgcctttct tctgttcagg aatttcagca tgacaagact gattcaggtt ttcataagcc
      961 tgatgcttcc gtttctattc caatggagtc tcggaggttt catatcaagc ggtgctgtga
     1021 tgctctgggc agctcttgca ataataggct ctcttacttt cggaaatgca aaatcaagca
     1081 tcacctggct cattctttat acccttctga cagttttttc aggaattatt gacgggagcc
     1141 tccctggttt caaggaatca ggatctgatt ctctg
//
LOCUS       DAOQGJ010001683         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101020_length_1175_cov_3.070536, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001683 DAOQGJ010000000
VERSION     DAOQGJ010001683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101020_length_1175_cov_3.070536"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1175
                     /locus_tag="PK683_20665"
     CDS             <1..>1175
                     /locus_tag="PK683_20665"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH10921.1"
                     /translation="KALTGYDRVMVYRFDEEFNGKVIAEAKEPEMVSYLNLHYPASDI
                     PVQARDLYRKNMIRTIKDVNYTPVPIDSKYSDPLDMSHSFLRSVSPIHLEYLRNMGVA
                     ATLTISIMVNHELWGLIGCHHRTPFLPGNKRLSMSEIFGSILGGIIQMREENQNEKRK
                     TELIARIDTVMEVILSQDKKIDLLDLIKKKIFLFQSIFPSDGFCIYSENSFVEHLFPY
                     SKNEIADLLHRIRPSMKDDCFSTDHLASIYPDFTENFLKMCAGLMILRIDTHPESFWI
                     WRRMEKRETISWGGDPNQKAVLNSEGIISPRKSFEDYNQVVMNKSVSWDAAEKEIGRY
                     LIPQIQRLFSFFESGRQLESQKIQIKSMEEEKARHYQELIEMLVDVIEQRDAYTAGH"
BASE COUNT          384 a          229 c          258 g          304 t
ORIGIN      
        1 tcaaggcact gaccggatac gacagagtca tggtctatcg ttttgatgaa gaattcaacg
       61 gcaaggtgat tgcagaagca aaagaaccgg aaatggtttc ttatttaaat cttcattatc
      121 ctgcaagcga tatccctgta caggcaagag atctgtacag aaaaaatatg attcgtacaa
      181 tcaaggatgt aaattacact ccggtaccta ttgacagcaa atactcagat ccactggata
      241 tgagccacag tttccttaga agcgtatccc ctattcatct ggagtatctg aggaatatgg
      301 gcgtggctgc aactctcaca atttccatta tggtcaatca tgaactgtgg ggactgatcg
      361 gatgtcatca cagaactcct tttcttccgg gaaataaaag actgagtatg tcggagatat
      421 tcggaagcat actgggcgga atcattcaaa tgagagagga aaatcagaac gaaaaaagaa
      481 aaacggaact gattgcaaga attgacacag tgatggaagt catactctct caggataaaa
      541 aaattgatct tctggatctg atcaaaaaaa agatatttct gtttcagtcc atatttcctt
      601 ccgacggttt ctgcatttat tctgaaaatt ccttcgtcga acatctattc ccttattcca
      661 aaaatgaaat tgctgatctt ctgcacagaa tcagaccgtc aatgaaagac gactgtttca
      721 gtacagacca tctcgcttcc atatatcctg atttcactga aaacttcctg aagatgtgtg
      781 caggactgat gattctccga atcgacactc atcctgaaag tttctggatc tggagaagaa
      841 tggaaaaaag agaaacaatt tcctggggcg gggatccaaa tcagaaagca gttttgaaca
      901 gtgaagggat tatttcccca agaaagtcct tcgaggatta caatcaggtg gtaatgaaca
      961 agtccgtctc ctgggatgcg gcagaaaagg aaatcggcag gtatctgatt cctcagatcc
     1021 agagactgtt cagttttttt gaatcaggaa gacagctgga aagtcagaaa atccagatca
     1081 agtcaatgga agaggaaaag gcaaggcatt atcaggagct gattgaaatg ctcgttgatg
     1141 tcattgaaca gagagacgcc tacactgcag gtcat
//
LOCUS       DAOQGJ010001684         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101048_length_1175_cov_2.618750, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001684 DAOQGJ010000000
VERSION     DAOQGJ010001684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101048_length_1175_cov_2.618750"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PK683_20670"
     CDS             complement(<1..1098)
                     /locus_tag="PK683_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNH10922.1"
                     /translation="MSKEKIIGIDLGTTNSCVAVMEGGDFVVIQNSEGARTTPSIVAF
                     TAKGETLVGQFAKNQAITNAVNTVRSAKRFIGRRYNEVTEEASKTSYKVIRSGNDGVK
                     FQTSAGEFTPQEISASVLQKMKKTAEDYLGHPVTKAVITVPAYFNDEQRQATKDAGRI
                     AGLEVERIINEPTAAALAYGFDKKKQNSKIAVYDLGGGTFDISILELGEGVFEVKATN
                     GDTHLGGDDFDQVIIDWLVEEFRKQTGIDISKDKNTVQRLKEAAEKAKIELSGTQSTQ
                     INLPFITADSTGPKHLDTTLTRAKFDELTKGLVERTRIPCMNCLRDSGLSTGDIDEVI
                     LVGGSTRIPAVQKLVKDLFGKEPNKSVNPDEV"
BASE COUNT          267 a          283 c          234 g          391 t
ORIGIN      
        1 cacctcatca ggattcactg atttgttagg ttcttttccg aaaagatctt tcaccagctt
       61 ctgaacagca ggaattcttg tggaacctcc caccagaatc acctcatcaa tatcgcctgt
      121 ggaaaggccg gaatcacgaa ggcagttcat acatggaatt cttgttcttt ctacaagccc
      181 tttagtcagc tcgtcaaatt ttgcacgggt cagagtcgta tccaggtgtt tcggacctgt
      241 agaatcagct gtaatgaaag ggaggttgat ctgagtggac tgagttccgg aaagttctat
      301 ctttgctttt tcagcagctt ccttcagacg ctgaacagta ttcttgtcct tgctgatatc
      361 gatgcctgtc tgttttctga actcttctac aagccagtca atgattacct gatcaaagtc
      421 atccccgccg agatgagtgt ctccgtttgt tgcctttact tcaaatacgc cttcaccgag
      481 ttccagaatg ctgatatcaa atgttccgcc cccgagatcg tatactgcta ttttgctgtt
      541 ctgcttcttt ttgtcgaaac cgtatgcgag ggcagctgca gtcggttcat tgatgattct
      601 ttccacttcc agaccggcaa ttcttcctgc atcctttgtc gcctgtctct gttcgtcatt
      661 aaaatatgca ggaactgtaa tgacagcctt agtgacagga tgtcccagat agtcttctgc
      721 agtctttttc attttctgca gaacacttgc agagatttcc tgaggagtga attcacctgc
      781 agaggtctga aatttcacac cgtcatttcc tgatctgatg actttatagg aagtcttgga
      841 agcttcctct gtaacttcat tgtagcgtct gcctatgaat ctcttggctg aacggactgt
      901 gtttacggca tttgtgattg cctggttttt tgcaaactgc ccaaccagag tttctccctt
      961 tgcagtgaat gcaacgatgg aaggtgtagt tcttgcaccc tcagaattct gtatcacaac
     1021 aaaatctcct ccttccatta ctgcaacgca tgagtttgtt gttcccaagt ctataccgat
     1081 gattttttct ttagacatac attcctcctg attaaaattt tggtctgcct attcttactc
     1141 ttgctggacg cagaactaca gattcattat tttct
//
LOCUS       DAOQGJ010001685         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101071_length_1175_cov_2.294643, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001685 DAOQGJ010000000
VERSION     DAOQGJ010001685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101071_length_1175_cov_2.294643"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PK683_20675"
     CDS             complement(<1..791)
                     /locus_tag="PK683_20675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH10923.1"
                     /translation="MSNPRISVIIPTLNEEKYLPILLHSLKQQTFRDFEVIVADAGST
                     DKTKEVAEGLGAKLVPGGMPGPGRNRGAEAANGDLLFFFDADVVLPNTFLEKAYAEME
                     EKMMDLATCEFKPDSKLQLDNILFKLANLTVRINQKFNPRAAGFCIFISRRLFRRVGG
                     FDESVKIAEDHDLVDRATKFRPLNFLNSVNLTVSIRRLEKEGRFSLIEKYMQVEMHLL
                     TKGSVKEDIIEYEFGKFDDEKKSQNKKILDDIEDRIIKFENQYRDL"
BASE COUNT          319 a          256 c          224 g          376 t
ORIGIN      
        1 agatccctgt actgattttc aaacttgatg atcctgtctt caatatcgtc cagaatcttc
       61 ttgttctgtg atttcttttc atcatcaaac tttccgaatt cgtattcaat gatgtcttcc
      121 ttgacgcttc ccttcgtgag cagatgcatt tcgacctgca tatacttttc gatgagagaa
      181 aaacgtcctt ccttttccag acgtctgatg ctcactgtca ggttgactga attcaggaaa
      241 ttcaagggtc tgaattttgt cgctctgtct acaagatcat gatcctcagc aatttttaca
      301 gattcgtcaa atccgccaac tcttctgaaa agtcttctgg aaataaaaat gcagaatccg
      361 gcagctctcg gattgaactt ctgattgatc ctgaccgtaa gatttgccag tttgaaaagt
      421 atattgtcca gctgaagttt ggaatcgggc ttaaattcac aggtagccag atccatcatc
      481 ttctcttcca tttcagcata ggctttttcg aggaatgtat tcggaagaac cacgtccgca
      541 tcaaagaaaa aaaggaggtc gccgtttgcc gcctctgccc ctctgtttct tcctggtcca
      601 ggcattccgc ccggaacaag ttttgccccg agaccttctg ccacttcctt tgttttgtcg
      661 gtggagcctg catccgcaac gatgacttcg aagtctctga aggtctgctg tttgagggaa
      721 tgaaggagga taggaagata tttctcttca ttcagagtag gaatgatcac gctgattctt
      781 ggattggaca tattgagaat ctttcttttc caattttatt ttgcaagtga aaattaagaa
      841 ggttttcagg aaaaaagagg ttaacccttt tcctgtgatt cggcttcaat tccgctccct
      901 aatttcggct tttaatgtta taatcaaatt ccttataaac cattactgaa gaaatgcagg
      961 tgcggccaaa tgttcattga ccggattata taggatggga aggcgaccgt tctccataac
     1021 ggacaagaat tcttctcttt tcagaaaaca caggaaagac agcaagaata aaaacaatca
     1081 gacttcggaa aataatattt cttctctgat ccaggaaatt tccttatcca gatatccaat
     1141 tcccagaagt ataatttttt ttactaagtt catat
//
LOCUS       DAOQGJ010001686         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101169_length_1174_cov_3.614835, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001686 DAOQGJ010000000
VERSION     DAOQGJ010001686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101169_length_1174_cov_3.614835"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK683_20680"
     CDS             complement(<1..267)
                     /locus_tag="PK683_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10924.1"
                     /translation="MTKIQMNRIIQKFGFFVTSFKPDQLTQHKGKIASMNSDKDISRV
                     AESESSFLDNLISSYMSDYDLEKENSKRSCLCRMKEPENLPFGID"
     gene            332..>1174
                     /locus_tag="PK683_20685"
     CDS             332..>1174
                     /locus_tag="PK683_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10925.1"
                     /translation="MSEHLRLNHLKETIIENSEKWISQFIRFSNGDSDLRRSLLYVFW
                     MSQDIPKIVQSINENDMDQQFITDLIYYGFGRQISMNLDAQEAMLNATKGLSSKKSLQ
                     LLNESPIIDIDSGFALALVIQLSSMDFSDFISSEKNRDFLRFIFENLEDSEIKLLFQR
                     SIQFYYNFVSLVRDREEFSDIMLKFQDDLALFHSITEIIKQIQSELEIRMNQPDQAAE
                     FRNFRLNLIWNALKQSSHKKLLLQIIAKEGIIKEETELKILEYFTVHSDFLAKKLHLS
                     RNDPF"
BASE COUNT          382 a          221 c          231 g          340 t
ORIGIN      
        1 gtctatgcca aaagggagat tctcaggttc tttcattctg caaagacaag atctcttact
       61 gttctccttc tctaaatcat aatcggacat ataactggaa atcagattat ccaggaatga
      121 tgattccgat tcagcgactc tgcttatgtc tttgtctgaa ttcattgatg caatttttcc
      181 tttatgctga gttaactgat ccggcttgaa tgaagtgaca aaaaatccaa atttctgaat
      241 gattctgttc atctgaattt ttgtcactta ctgtctttca gaccggtata tataacagct
      301 gcagaaaaaa actcaaggaa gcgaagtcaa aatgtcagag cacctcaggt tgaatcacct
      361 caaggaaaca atcatagaaa attctgaaaa atggatcagc cagttcatcc gcttttcgaa
      421 cggggattca gatttaagga gaagccttct ttatgtgttc tggatgtctc aggacattcc
      481 caaaattgtg caaagcataa atgaaaacga tatggatcag cagtttatca ctgacctgat
      541 ctattacgga ttcggaagac agatcagcat gaatctggat gcacaggagg caatgctgaa
      601 tgccacaaag ggactcagtt ctaaaaaaag tctgcagctt ctgaatgaaa gcccgattat
      661 tgacattgat tcaggctttg ccctggcact ggtcattcag ctgagcagta tggatttttc
      721 agatttcatt tcctctgaaa aaaatcggga cttcctgaga ttcatatttg aaaatctgga
      781 agactctgaa ataaagctgc tctttcaaag aagtatacag ttttattata attttgtaag
      841 ccttgtgagg gacagagaag agttcagtga cattatgctt aaatttcagg atgacctggc
      901 actgtttcac tcgattactg aaatcataaa acagattcag tcagaactgg aaataagaat
      961 gaatcagccg gatcaggcag ctgaattcag aaacttcaga ctgaatctga tctggaatgc
     1021 actgaagcag tccagtcaca aaaagcttct gcttcagatc atagcaaagg aaggaattat
     1081 caaagaggaa actgaattga aaattctgga atatttcaca gtgcattctg attttcttgc
     1141 aaaaaaactg cacttgtcca gaaatgatcc attc
//
LOCUS       DAOQGJ010001687         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101177_length_1174_cov_3.495085, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001687 DAOQGJ010000000
VERSION     DAOQGJ010001687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101177_length_1174_cov_3.495085"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PK683_20690"
     CDS             <1..884
                     /locus_tag="PK683_20690"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH10926.1"
                     /translation="SGGSGFIGRNLISHLAGKAEIVFLGRSLPEDLSGKITEYVYWDI
                     LSGKPPRKNVSGDILIHLAGIPFGEEGSISDYRIGNTESVRIILSYMKKNEIPRILFA
                     SSVSVYGNSEGKPDESSPLLGTSGYAVSKIEAEKLIITSRLKYGIFRIASVYGEGGKS
                     FLNKLIRMSRRGFVPYPRSGKTRKSFIHSDDLSCFMISYINKNISGIWNICHPEAADF
                     SEILALFEEIQGRKLWRAPVPGFFLKFEGIYKLLSGKDFPLKPLLSDSAFSSDKAVRD
                     LEYRPAVSLKEGLRRLV"
BASE COUNT          344 a          225 c          251 g          354 t
ORIGIN      
        1 tatcaggcgg atcaggtttc atcggcagaa acctgatcag tcaccttgcc ggtaaagctg
       61 aaattgtttt tttaggcaga agtcttcctg aagatctttc cggaaaaatt acagagtatg
      121 tatattggga tatactgtca ggaaaacctc ccagaaaaaa tgtctccgga gacatactga
      181 ttcatctggc aggaattccg ttcggcgagg agggaagtat ttccgattac aggattggaa
      241 atacggaatc tgtcagaatc attctcagct atatgaaaaa aaacgaaatt cccaggatac
      301 tttttgccag ttcggtctct gtctacggaa actcagaagg aaaaccggat gaatcgtctc
      361 ctctgctcgg tacctcaggt tatgcagtat ccaagattga ggcagaaaaa ctcattatta
      421 cctcgcgtct gaaatacggg atattccgta ttgcatcggt ttacggcgag ggcgggaaaa
      481 gtttcctgaa taagcttatc aggatgtccc gcaggggatt tgttccttat cccagatcag
      541 gaaagaccag gaaaagcttc atacattccg atgatctttc ctgtttcatg atttcatata
      601 taaataaaaa tatttcagga atctggaaca tctgtcatcc ggaggcggcc gatttttcgg
      661 aaattcttgc actgttcgaa gaaattcaag gaagaaagct ctggagagct cctgtcccag
      721 gcttttttct taagtttgaa ggaatttata aattgctgtc aggaaaagat ttccccctga
      781 aacctctcct ttcagattcc gcattctctt cagacaaggc tgtcagggat ctggaataca
      841 ggcctgcagt ttcgcttaag gaaggtcttc gaagactcgt ataagtttcc tgcgtttcca
      901 aaagtccaac tttttcgtga agcaggagga aaaaatgaga tctcttgaag atgcttcatt
      961 ttatgcgatt tttcaaaagt tcctgcgcat atattctttt tcggctcaat agaatcatgg
     1021 ctgtcggcat aaaagagttt tcgtatggac tgtgcagtga gaagtttata aatataatat
     1081 agttatacta tatttttata gaatgaaaaa tatagtataa cttctgattc tgtgaatcag
     1141 aatatgcaag ctgcattgtc tgcactctta attt
//
LOCUS       DAOQGJ010001688         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101257_length_1174_cov_2.331546, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001688 DAOQGJ010000000
VERSION     DAOQGJ010001688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101257_length_1174_cov_2.331546"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..703
                     /locus_tag="PK683_20695"
     CDS             26..703
                     /locus_tag="PK683_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10927.1"
                     /translation="MISIIKKERNTLSGTDFIDHAKKKYQKETDADIAELLGITQPAL
                     NGWKNKGLTFRSIFEVFEKGKKAHTKTIVTPVIEFFPVSRMESRHGVKYEILDTSQDS
                     HGLLQKELNSHNGVYIFYDSKGAAIYVGKAKKQSLWKEMNLAYNRKRDSQTVRRVKHL
                     KINRFNPKKEKRRQIKSYKIYLHDMAAYFSAYRADSAFIDSLEAALIRMFPNNLLNKK
                     VEQFKGL"
     gene            890..>1174
                     /locus_tag="PK683_20700"
     CDS             890..>1174
                     /locus_tag="PK683_20700"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNH10928.1"
                     /translation="MKIEDLWKDKNYEPNDEQKNSILHTNGPLYITAAPGSGKTAVLV
                     WRTLYLIVFQNVKPEKIFLATFTEKAAFQLKERLQSLLSLVAEKSNGTKGP"
BASE COUNT          396 a          211 c          234 g          333 t
ORIGIN      
        1 gatcgcttca agagagtgat tattcatgat ttctattatc aaaaaagaaa ggaacactct
       61 atccggcact gacttcattg atcacgcgaa gaagaaatac cagaaggaga cggatgcaga
      121 cattgcagaa cttctgggta ttactcagcc agctctgaat ggctggaaaa ataagggact
      181 cacatttcgt tccattttcg aagtctttga aaagggaaag aaggctcata caaaaactat
      241 agtgactccg gttattgaat ttttccctgt cagccgtatg gaatccaggc acggagtcaa
      301 atatgaaatt ttggacacat cacaggattc tcatggactc ctgcagaaag aactgaattc
      361 gcacaatggt gtttatatct tttatgattc taaaggggca gccatttatg taggcaaggc
      421 gaaaaaacag tctctttgga aagaaatgaa tcttgcatat aacagaaaaa gagattctca
      481 gacagtccga cgcgttaaac atttgaagat aaaccgtttc aatccgaaga aggaaaaaag
      541 aagacagatt aaatcatata agatttacct gcacgatatg gccgcctatt tttcagccta
      601 tcgggctgat tcagccttta tagacagtct ggaagcggca ctgattcgaa tgttccctaa
      661 caatctttta aacaaaaaag tggaacagtt taaaggttta taaacagaaa gtgtcaatct
      721 gctttgactg atcgaatctg aagttctgat attttcagac atttcgtttt tccgcaaaaa
      781 atgccttttc caatgtaaaa gtattgcaaa tagatcgaag ggcaatatct tgctcattta
      841 tgatgaattg atttttcagt tcattttgat ccattcgtga ggggctgtga tgaaaattga
      901 agatctttgg aaagacaaga actatgaacc gaatgatgaa cagaaaaact caatacttca
      961 tactaatggt cctttatata ttactgcggc tcccggttca ggaaagacag ctgttttagt
     1021 gtggagaact ctatatctaa ttgtttttca aaatgtaaaa cctgaaaaaa tttttttggc
     1081 gactttcaca gaaaaagcag catttcaatt gaaagagaga ttgcagtctc tcctctctct
     1141 agtcgcagaa aaatcgaacg gaaccaaggg tcct
//
LOCUS       DAOQGJ010001689         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101353_length_1173_cov_3.532200, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001689 DAOQGJ010000000
VERSION     DAOQGJ010001689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101353_length_1173_cov_3.532200"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /gene="sucB"
                     /locus_tag="PK683_20705"
     CDS             <1..694
                     /gene="sucB"
                     /locus_tag="PK683_20705"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNH10929.1"
                     /translation="ETVVPMTRLRRAIADRLLSAQHNAAILTTFNEVDMSAVMNLRNQ
                     YKDRFKEEHNVGLGFMSFFTKAAVSALKAFPAVNGEIRDNNIVYKNYYDIGVAVGGPK
                     GLVVPVIRDADLLSFAGIESEILRMAGKVKDGTITLEEMQGGTFSISNGGVYGSMMST
                     PILNPPQSGILGLHNIVKRAVAVNDQIVIRPMMYLALSYDHRIIDGKEAVQFLVRIKE
                     AVEDPTRLLLEV"
     gene            694..>1173
                     /locus_tag="PK683_20710"
     CDS             694..>1173
                     /locus_tag="PK683_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH10930.1"
                     /translation="MMKEFDTVVIGSGPGGYVAAVRAAQLGQKTAIIEKRATLGGTCL
                     NVGCIPSKALLDSSEAYHQTLHKLSDHGVNVTDVKLDLKKLLGRKDKVVKEVCDGVDY
                     LMKKNKIERFLGFGSLKSANEIEISSDSGKEIIKAANIIIATGSVPIDIPGFTVDGKQ
                     "
BASE COUNT          364 a          240 c          279 g          290 t
ORIGIN      
        1 agagacagtt gttccgatga caagactccg tcgtgcaatt gccgacagac ttctttctgc
       61 acagcacaat gccgccattc tcactacctt caatgaagtc gatatgtccg ctgtaatgaa
      121 tctgagaaat cagtataagg acagattcaa ggaagagcac aatgtaggac tcggattcat
      181 gagtttcttt acaaaggctg cagtatcggc actgaaggct ttccctgccg tgaacggtga
      241 aatcagagat aataatatag tttataaaaa ttactatgat ataggggttg cagtaggcgg
      301 tccgaaaggt ctggttgttc ctgtgatcag agatgcagat cttttaagtt ttgccggaat
      361 agaatccgaa atcctcagaa tggcaggcaa agtcaaagac ggcacaatca ccctcgaaga
      421 aatgcaggga ggaactttct ccatttccaa cggaggcgtt tacggttcca tgatgtctac
      481 acctattctg aatcctcctc agagcggaat attaggactt cataatatag tcaagagagc
      541 cgtcgcagtc aacgatcaga tcgtcatcag accgatgatg tatcttgctc tttcctatga
      601 ccacagaatc atagacggaa aggaagcagt tcagttcctc gtcagaataa aagaagcagt
      661 tgaagatccg acacgtcttc ttctggaggt ctgattatga aagaatttga tacagttgtc
      721 ataggatcgg gccccggagg ttacgttgct gcagtcagag cagcgcagct cggtcagaaa
      781 acggccatca ttgaaaaaag agcaaccttg ggcggtacct gtctgaatgt aggatgcatt
      841 ccttcaaaag ctcttctgga ttcgtctgaa gcctaccacc agacactgca taaactttca
      901 gatcacggcg tgaatgtaac tgacgtgaaa ctcgatctga agaaacttct tggaagaaag
      961 gacaaggtag tcaaggaagt ctgtgacgga gtagactatc tgatgaaaaa gaacaagatt
     1021 gaaaggtttc tgggattcgg ttcgctgaaa agtgcaaatg aaattgaaat cagttcggat
     1081 tcaggaaaag aaattataaa agcggcaaat atcattattg caaccggatc tgtacctatc
     1141 gacattccag gcttcactgt agacggaaag cag
//
LOCUS       DAOQGJ010001690         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101361_length_1173_cov_3.327370, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001690 DAOQGJ010000000
VERSION     DAOQGJ010001690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101361_length_1173_cov_3.327370"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PK683_20715"
     CDS             complement(<1..692)
                     /locus_tag="PK683_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004767433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HNH10931.1"
                     /translation="MNFGILKENEDETRVSLLPEGVEALIKMGHKVNLESGAGESAGA
                     SDESYRKVGATLTNRSEVLDKSECVLKITPPTAEEISKLRPGLAIMGVYSPLSNFQLI
                     KTLAEKNISVFSFDMIPRTTRAQSMDVLSSMSTISGYKAVLLAASSLPRFFPMLTTAA
                     GTIAPARALVLGAGVAGLQAIATARRLGAVIEAFDTRPEVKEQVMSLGAKFVEVEGAK
                     DASGAGGYAVEQT"
     gene            complement(841..>1173)
                     /locus_tag="PK683_20720"
     CDS             complement(841..>1173)
                     /locus_tag="PK683_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10932.1"
                     /translation="SAMNYDNILQYFNDHPEDPQGKNMGLAFSLILLKESGLRPELMR
                     VSKSGKGAFSRIEIPFVDNYSSIRDRILRDESIVPFEKPNLIPPEFREKFEERQRQLK
                     NNGILPVQ"
BASE COUNT          318 a          286 c          229 g          340 t
ORIGIN      
        1 gtctgctcca ccgcataccc gccggcaccg gaggcatcct ttgctccttc cacttccacg
       61 aacttggcac cgagactcat tacctgctct tttacttccg gcctagtatc aaaagcttca
      121 atgactgcac ccagtcttct cgctgtagca attgcctgca gtccggccac tccggccccg
      181 agcacaagtg ctcttgcagg agcaattgtt cctgctgcag ttgtcagcat aggaaaaaaa
      241 cggggaagtg atgaagcagc cagcagaaca gccttgtatc cggaaattgt gctcattgag
      301 gaaagaacat ccatactctg cgctcttgtg gttcttggaa tcatatcaaa actgaataca
      361 gatatattct tttctgccag agtttttatg agctggaaat tagaaagagg agaataaact
      421 cccataattg caagacctgg acgaagttta gaaatctctt ccgctgtagg aggagttatt
      481 ttcagaacac attcagactt gtccagaact tccgatctgt ttgtcaatgt ggctccaacc
      541 tttcggtaag attcatcaga agcaccggcc gattcccctg caccggattc caagtttact
      601 ttatgcccca ttttaatcag agcttccaca ccttccggaa gaagggagac cctagtctca
      661 tcttcgtttt ctttcagtat cccaaaattc attctgtcac cgtcgaaaag ccgtcaaaca
      721 gcctttattc tgaactgctc agtcaatcat ccaaaacagg aaaaaattta cttatgcaat
      781 tctaaagtca aagtcccttg ttctcggaaa aatgcaataa actttcggaa tatgaatttc
      841 ttattgtaca ggaagtatcc cgttgttctt caactgtctc tgtctttctt caaatttctc
      901 tctgaattca ggagggatca gattcggttt ttcaaaagga acaatggatt cgtctcggag
      961 aattctgtct ctgatgctgg agtaattgtc tacaaaagga atttcaattc tggaaaatgc
     1021 acccttccct gacttactga ctctcatcag ttcaggcctg agtccggatt ccttcaggag
     1081 tatcagagaa aatgcaagac ccatattttt tccctgagga tcttcagggt gatcattaaa
     1141 atactgcaga atattgtcgt agttcattgc aga
//
LOCUS       DAOQGJ010001691         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101371_length_1173_cov_3.087657, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001691 DAOQGJ010000000
VERSION     DAOQGJ010001691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101371_length_1173_cov_3.087657"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="PK683_20725"
     CDS             complement(<1..1132)
                     /locus_tag="PK683_20725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH10933.1"
                     /translation="MLSQALLLAERFNQDYISFRETAALLMKSNCELSALKENLEKKI
                     SERTQELEKARDSALASSRAKSEFLAVMSHEIRTPMNGILGMIQLLEMTELTEEQKDY
                     LSTLNFSTETLSAVIHDILDFSKIESGKTEITKEDFSLRVLMDDFIKFIRTQAEKNDL
                     SLEYHYDENLPEYLNGDSVRLKQILLNLSSNAVKFTEKGRIIISAAVNRIENRICTVE
                     FSVKDTGIGIPKDKQDRLFQMFSQADSSTTRKYGGTGLGLAISRKLAELMGGKIWVES
                     RENEGSEFRFTVCLEISENRKQDFLKKESTIRPDLKILAAEDNRTNQKMILHLLAKLG
                     CSQCEIAENGKIVVDLLKSGKTYDLILMDIEMPELDGLEAAAE"
BASE COUNT          268 a          260 c          236 g          409 t
ORIGIN      
        1 tttcagccgc tgcttccagt ccgtccagtt cgggcatttc aatgtccatg agaatcagat
       61 cataggtttt tcctgatttc agcaggtcga cgactatctt tccgttttcg gcaatttcgc
      121 attgactgca cccgagtttt gccagcagat gcagaatcat tttctggttg gttctgttgt
      181 cttcagctgc aagaattttc agatcaggcc tgattgtgct ttcctttttc agaaaatcct
      241 gctttctgtt ttctgagatt tccagacaca ctgtaaaacg gaattcgctt ccctcatttt
      301 ctctgctttc cacccagatc ttcccgccca tcagttctgc aagttttctg ctgattgcaa
      361 gacccagccc tgttcccccg tattttctcg tcgtggagga gtctgcctga gagaacatct
      421 gaaagagtct gtcctgcttg tctttcggaa ttcctattcc agtatccttt acgctgaact
      481 ccactgtgca tatcctgttt tctatccggt tcactgcagc agaaataatt attctgcctt
      541 tttcagtgaa cttgactgca ttgctgctca ggttcagcag aatctgtttc agtctgacag
      601 aatccccgtt cagatattcg ggaagattct catcgtaatg atattctaat gacagatcat
      661 ttttttctgc ctgagttctt atgaatttta tgaaatcatc catgaggacg cggagtgaaa
      721 agtcttcttt cgtgatttca gtctttccgg attcaatttt tgaaaaatcg agtatgtcat
      781 gaatgactgc tgaaagagtc tctgtgctga aattgagagt ggaaagatag tctttctgct
      841 cttcagtcag ttcagtcatt tccaggagct ggatcattcc gagaattcca ttcataggtg
      901 ttcttatttc atgactcatc actgcaagaa attcagattt tgcacggctg gatgcaagag
      961 ccgaatctct tgctttttca agctcctgtg ttctttcgga tatcttcttt tccagatttt
     1021 ctttcagagc agagagttca cagtttgatt tcatcagaag tgctgcagtt tctcggaagg
     1081 atatatagtc ctgattgaat ctttctgcaa gtaaaagtgc ctgagaaagc ataaaaatat
     1141 atcctgagta gtgtcctatg atgtttggag tgt
//
LOCUS       DAOQGJ010001692         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101503_length_1172_cov_3.677708, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001692 DAOQGJ010000000
VERSION     DAOQGJ010001692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101503_length_1172_cov_3.677708"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK683_20730"
     CDS             complement(<1..534)
                     /locus_tag="PK683_20730"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH10934.1"
                     /translation="MILQGTGASVYTEPGNSVLTAPSSENPTLLTASSGTSSCNDAAV
                     EPPASFGSAGPEAFYSVCSSENVNRFKNLEIKFSHPVNQSTFAASFSVMCNGSALPGP
                     APGGALIWKSPQRLIFDPYRELPSNASCVITIANSAETIEGEKLISYSKNFSASHDYL
                     ISATVTQGPTSIPLGGTN"
     gene            911..>1172
                     /locus_tag="PK683_20735"
     CDS             911..>1172
                     /locus_tag="PK683_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10935.1"
                     /translation="MKNTINVLLLSGILCFSGLSAVQVTESRLKVKSGKILYTLVKTE
                     WETTAKMSGVGEKFSGTVDVDGKAVSITASLKRDSFYLTGEYK"
BASE COUNT          356 a          198 c          274 g          344 t
ORIGIN      
        1 atttgttccg cccaggggaa ttgacgttgg tccttgagtg acagtggccg agataagata
       61 atcatgggaa gcactgaagt ttttcgaata gctgatcagt ttttcacctt caatagtttc
      121 cgctgaattt gcaattgtaa ttacacagga ggcattggac gggagttctc tgtaaggatc
      181 aaaaatcagt ctttgaggac ttttccaaat taaagcacct cctggcgcag gtcctggaag
      241 ggctgacccg ttgcacatta ctgaaaagga tgctgcaaaa gttgactgat taacaggatg
      301 ggaaaatttt atctcaagat ttttgaatct gtttacattc tccgaagagc atacgctgta
      361 aaatgcttca ggtcctgccg atccgaaaga tgccggaggt tcaacggctg catcattgca
      421 ggaacttgtg ccggacgaag cagtcagaag ggtcggattt tctgaagacg gtgcagtaag
      481 aacagaattt cccggttcag tataaactga tgcccctgtt ccctgcagta tcaaaggaag
      541 tatctgagaa attcctttat ttcctgcaga atcacaccca gcgtgaaaaa aaaccaaaat
      601 cggcaataag taaatagagc tgagaagccc ttttttaggt gaatttttca ttttttactt
      661 cctttttcta atgctgttaa ttatagcttg ttttcagagt gaacaagtgt ttattttttg
      721 taaacaattt tttacttgtt atgctaaaaa aattctaaag tccgtaagtg acataacttg
      781 gaatggcctg tctgggaaga tttttatcat agtgtcggct ttgataatag tattgcagta
      841 agcaggatgg aatgtataac agatataaaa tttcagtatc gtttcagaaa acggaatggg
      901 gaaaagcttt atgaagaata caataaatgt gttattactg tcagggattt tatgtttttc
      961 gggattgagt gcagttcaag tcactgagag ccgacttaaa gtaaagtcgg gaaagattct
     1021 gtacacgctt gtaaaaacag agtgggaaac cactgcaaag atgtcgggag taggtgagaa
     1081 attcagcgga accgtagatg tagacgggaa agcagtcagc attactgctt ccttgaaaag
     1141 agacagtttc tatctgacag gcgagtataa gt
//
LOCUS       DAOQGJ010001693         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101718_length_1171_cov_2.691756, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001693 DAOQGJ010000000
VERSION     DAOQGJ010001693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101718_length_1171_cov_2.691756"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PK683_20740"
     CDS             complement(<1..535)
                     /locus_tag="PK683_20740"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH10936.1"
                     /translation="MSNKADVIVIGAGVSGLAAAYDLVKAGKKVIVLEGRNRIGGRQW
                     TDRTWKNSPMDMGASWIHGIKKNPVTKLAEEFKMKMIVTSQENAVLYDENGKEISDEE
                     FEELESDLETVLKAVSKKRKNTEKDVSLGSMMEKYMEEEECSEEEKKRIRFAAYSAIE
                     NDYAADCSDLSFLNWDED"
     gene            complement(616..>1171)
                     /locus_tag="PK683_20745"
     CDS             complement(616..>1171)
                     /locus_tag="PK683_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10937.1"
                     /translation="SLGEITDDLGLLRIEFKPEPEERIVNYSGQYFDLMREAVSDYRS
                     GSLIEAEEKLAGILLYDPENLDMLRIYCKTLLKNRRYAKAVGFIETYLQKRPSDSKFL
                     YYCSFCMKQTGRYTEAAEYGERYMLREPVDSTAAAELAEIYCLSENWESAGIMLEEAF
                     RLDPSSIKAEKLRKQMFDKRLISV"
BASE COUNT          269 a          298 c          211 g          393 t
ORIGIN      
        1 catcctcatc ccagttcaga aaggaaagat cagaacagtc tgcagcgtag tcgttttcaa
       61 ttgcactgta ggcggcaaaa cggatgcgct ttttttcctc ttcagaacat tcttcctctt
      121 ccatatattt ttccatcatt gatccgagag agacatcctt ttctgtattc ttcctttttt
      181 tacttacggc tttcagcact gtttccagat cagattccag ttcctcaaat tcttcatctg
      241 aaatttcttt tccgttttca tcataaagaa ctgcattttc ctgagatgtg acgatcattt
      301 tcattttgaa ttcttcagcg agtttagtga ctggattttt tttgatcccg tgaatccatg
      361 aggcacccat atccatcgga ctgtttttcc aggttctgtc cgtccactgc cttccgccga
      421 tcctgtttcg tccttcaagg acaatgactt tttttcctgc ttttaccaga tcatacgccg
      481 ctgcgagtcc ggatactcct gcaccgatca ctatcacatc tgccttattg ctcatactgt
      541 cctcctgaaa atgcaatggt taagatattg accggttctc actgtgcaag tatttttatt
      601 ctgcagacag ccttttcata ctgagataag tcgtttgtca aacatctgct ttctcagttt
      661 ttccgctttg atgctcgacg gatccagtcg aaatgcttcc tcaagcataa ttcctgcaga
      721 ttcccagttt tctgaaagac agtagatctc agcaagttcc gctgctgcag tgctgtcaac
      781 aggttcccga agcatatacc tctccccata ttcagctgct tcagtatatc ggcctgtctg
      841 cttcatgcag aaggagcaat aataaagaaa cttagaatct gagggtcttt tctgaagata
      901 cgtttcaatg aagcccaccg cctttgcata ccttctgttt ttcagaagag tcttgcagta
      961 gattcgaagc atatccagat tttccggatc atacagaagt attcctgcca gtttttcttc
     1021 tgcttcaatg agactgccgg atctgtaatc cgagacagcc tctctcatca gatcaaaata
     1081 ctgtccgctg tagttcacga ttctttcttc cggttccggc ttgaattcaa tccggagaag
     1141 tcccaggtcg tctgtaattt ctccgagact g
//
LOCUS       DAOQGJ010001694         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101721_length_1171_cov_2.656810, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001694 DAOQGJ010000000
VERSION     DAOQGJ010001694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101721_length_1171_cov_2.656810"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK683_20750"
     CDS             complement(<1..294)
                     /locus_tag="PK683_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HNH10938.1"
                     /translation="MTDFRRIAEEKIADLTEKLTRVRDQHFIRKLQLGLVLITMGLSA
                     YYLSYPFFGQTEIDLRPNGPYTIGSVSTETVISRREINYIDENRTQAERAKAYN"
     gene            complement(291..1136)
                     /locus_tag="PK683_20755"
     CDS             complement(291..1136)
                     /locus_tag="PK683_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNH10939.1"
                     /translation="MEIIPRGISFIISGPKDKIAHTIDFLKKLEANFSGRPDADDLDS
                     FDLSYQIRNHKQEGSPWNPSEKILTTNKGKAIYPRTVNQEKFVQSLQKNIITFASGPA
                     GTGKTFLSIAVASRMLQNGEVERLILTRPAVEAGESLGFLPGDMKEKVDPYLRPIYDA
                     LNECIGYEKVQVLTSLGKIEIAPIAFMRGRTLANSFIILDEAQNCTAMQLKMILTRIG
                     KNSKMAVSGDITQIDLAKDRSGFQKVAGLLSDIPEVGSVVFGKEDITRHYIIEAIVSR
                     LEKLL"
BASE COUNT          296 a          282 c          238 g          355 t
ORIGIN      
        1 attgtatgcc ttagcccttt ctgcctgtgt cctgttttca tcgatataat ttatctcgcg
       61 tctggaaatc acagtttctg tcgaaacgga accgattgta tacggtccgt tcggacgaag
      121 atcaatttcg gtctgtccga aaaaaggata agaaagatag tatgcagaga gccccatcgt
      181 aatgagaacg agcccaagct gaagttttct gatgaaatgc tgatcgcgca ccctggtcag
      241 cttctcagtc agatcagcta tcttctcctc tgcaatacga cggaaatcag tcataaaagt
      301 ttttcgagcc tgctcactat ggcttctatg atatagtgtc tggtgatgtc ttcctttccg
      361 aagacaacgg aacccacttc cggaatgtcg gaaagcagtc cagccacttt ctggaatcct
      421 gatctgtcct ttgcaaggtc aatctgagtg atatccccgg aaacagccat cttggaattt
      481 tttccgatcc tggtcagtat catcttcagc tgcattgcag tacagttctg agcctcatcg
      541 agaataatga aagaatttgc tagagtcctc cctctcataa aggcaatcgg agcgatttca
      601 attttcccga gagaagtaag aacctgaact ttttcatatc ctatgcattc attcagagca
      661 tcgtaaatcg gtctgagata aggatctact ttttccttca tatcccctgg aagaaaacct
      721 aaagactccc ctgcttccac tgcgggcctc gtaaggatca gtctttccac ctctccgttc
      781 tgaagcattc tgcttgccac tgcaatggac agaaaggtct ttcctgtacc tgcagggccc
      841 gatgcaaagg taatgatgtt tttctgaaga ctctgaacaa atttttcctg atttacagtt
      901 ctgggataaa ttgccttccc tttatttgta gtcagaattt tttcggaagg attccatggg
      961 cttccttcct gcttgtgatt tctgatctga tagctcagat caaaagaatc cagatcgtct
     1021 gcatccggtc tccctgaaaa atttgcttcc agctttttca aaaaatcaat cgtatgagca
     1081 atcttgtcct tcggaccgct gatgatgaat gaaatgcctc tcggtatgat ttccacaccg
     1141 agagattttt ccagctgtct gacacggctg t
//
LOCUS       DAOQGJ010001695         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_101848_length_1170_cov_3.300448, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001695 DAOQGJ010000000
VERSION     DAOQGJ010001695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101848_length_1170_cov_3.300448"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PK683_20760"
     CDS             complement(<1..943)
                     /locus_tag="PK683_20760"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH10940.1"
                     /translation="MKIRYKLILLIILTVLAVAVPISYVVITRSETIIVKNTYFICSN
                     LSESISNIAREELFLNTNYEGTEKVLQGLNASNFQGLYNVYIINIDGKYVADLRNREI
                     GKYLSDEELKYIQSIQNLSYRQIKNGNTSVLRFTYPIFLDHTDAKLKIGATVFDFEYD
                     LLFAPVSEMRAQILLFSASILLFSILTGVLFSYLLTKPVQLLSKGAKVIRAGSLDYRI
                     PLKGSDEIGQLAEEFNSMTAEIQRAQKDLKSLNENLEQKVKERTSELIKANEEAEIEK
                     KIPACPQACHLSRVASVQPLILRKGEAFLILIPKIASS"
     gene            complement(951..>1170)
                     /locus_tag="PK683_20765"
     CDS             complement(951..>1170)
                     /locus_tag="PK683_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10941.1"
                     /translation="RFPEYFRTGLEFYQKRKYKEAVDTFENTLLLDPAHQNSIEYLRL
                     SKMKYDAITSITNCSKHTQSGCRLLKSQ"
BASE COUNT          321 a          242 c          234 g          373 t
ORIGIN      
        1 gagagctggc tattttgggg attaggatca ggaaggcctc cccctttctc aagatcaacg
       61 gctggacgga agccactctg gagaggtggc aggcttgagg gcaggccgga attttctttt
      121 ctatctccgc ttcttcatta gccttgatca gttcagaagt tctctctttc actttctgtt
      181 ccagattttc attcaaagat ttcaagtctt tctgtgctct ctgaatttct gcagtcattg
      241 aattgaattc ctcggccagc tgtcctattt catctgatcc cttcaacgga attctgtaat
      301 ccaggctccc agccctgatg acctttgctc ctttggaaag aagctgaaca ggttttgtaa
      361 gcaggtagga aaagagaaca cctgtcagta ttgaaaaaag cagaattgat gcactgaaca
      421 gaagtatctg ggcacgcatt tcggaaaccg gtgcgaagag aagatcatat tcaaaatcaa
      481 agacagtggc gccgattttc agttttgcat ccgtatgatc cagaaagatt gggtaagtaa
      541 aacgcagaac actggtattc ccgtttttta tctgtctgta actgagattc tgtatggact
      601 gaatatactt cagttcctcg tccgaaagat attttcctat ttcccgattt cgaagatcag
      661 caacatattt tccatcaata tttattatat atacattata cagtccctga aagttcgacg
      721 cattcagacc ctgaagaact ttctcagtgc cttcatagtt tgtgttgagg aaaagctctt
      781 ctctggcgat gtttgaaatt gactccgaaa gattgctgca tataaagtat gtattcttta
      841 caataatagt ttcacttctg gtaatgacca cataagaaat tggaactgct acagcaagaa
      901 ccgtaagtat gatcaaaagt ataagtttat atctgatttt cataattgaa ttactgtgac
      961 ttcagaagtc tgcaccctga ctgagtgtgc ttggaacagt tcgttatgct tgtaattgca
     1021 tcatatttca ttttcgaaag cctgagatat tcaatggaat tctgatgcgc cggatcaagc
     1081 agaagtgtat tttcaaaagt atccactgct tctttatatt ttcttttctg gtaaaactcc
     1141 agccccgtcc tgaaatactc cgggaacctt
//
LOCUS       DAOQGJ010001696         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102002_length_1169_cov_3.939856, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001696 DAOQGJ010000000
VERSION     DAOQGJ010001696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102002_length_1169_cov_3.939856"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1169
                     /locus_tag="PK683_20770"
     CDS             <1..>1169
                     /locus_tag="PK683_20770"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH10942.1"
                     /translation="NWSDEVLNEFFNLVGYLRFIEHEYLLPIIDVDKHEGIPFIVFAD
                     DNFRLFNQAPINRRDEKELLGFISKIAEALDYLHKQELVHGNLQPENIVLDPNDFPVL
                     FDFGLSGVFKKLLLENMDEGFENLAISNLRYTSPEQILGRSPSRLSDIYMFGIVGYYA
                     IFNELPFDGKNSSETALSHLNDGVIKPLTLPDHISQNVLNLLQKCLQYKPDDRFESFT
                     QILVALEKLKNKKRTGIRFKKRFVIVQQSKGSRVSPTFIRVVASVIGVLFIALFFIFQ
                     NLRTSSVPMETQATKTPTIQKTATQPQWTKTETPVEPTVETLVLPSPTATVETYQLAF
                     ESEKPFDTSGQEISPTNLSNLTEISRLGYGRPEEMDVASDEIHVAVATSAGVLIF"
BASE COUNT          346 a          257 c          221 g          345 t
ORIGIN      
        1 gaactggtct gatgaagtgt tgaacgaatt cttcaacctg gttgggtatc ttcgttttat
       61 tgaacatgag tacctccttc cgattataga tgtagacaaa catgaaggca ttccatttat
      121 cgtttttgcg gatgataatt ttcgactgtt taatcaagcc cctatcaatc gcagggatga
      181 aaaagaattg cttggattta tctccaagat cgctgaagcc cttgattatt tgcataaaca
      241 agaattagtg catgggaatc tccagccgga aaatattgtt ctcgacccga atgattttcc
      301 tgttcttttt gattttggat taagtggggt tttcaagaaa ctgctccttg aaaatatgga
      361 tgaaggtttt gaaaaccttg ccatttccaa cttaaggtat acatccccag agcaaatcct
      421 gggacgaagt ccttcccgcc tttctgatat ctatatgttt ggtatcgtcg gatattatgc
      481 tattttcaat gaattgcctt tcgacggaaa gaactcatct gaaacagccc tctcacatct
      541 taatgatgga gtgatcaagc cccttactct accagatcac atatcgcaaa acgtcctaaa
      601 tctactccaa aaatgtcttc aatataagcc agatgatcgt tttgaaagtt tcacacaaat
      661 actggttgcc cttgaaaaat taaaaaacaa gaaacggaca ggtattcgat ttaagaaaag
      721 atttgtcatc gtccagcaat cgaaagggag cagagtttct ccaacattta ttcgagtagt
      781 cgcatctgtc ataggtgtcc tcttcatcgc tctgtttttt atttttcaga atttaaggac
      841 ttcttcagtt ccaatggaga cccaggcaac aaagacgccc actattcaaa aaacagcaac
      901 tcaaccccaa tggacaaaga cagaaacacc agtcgaacct accgtagaaa cgctggttct
      961 tccttcgcct actgcaacgg tcgaaactta tcagcttgcc tttgaaagtg aaaaaccttt
     1021 cgacacttcc gggcaggaaa tatctccaac gaatttatcc aacctgaccg aaatttcccg
     1081 actgggatat ggcagacccg aagaaatgga tgttgcctct gatgaaattc atgttgccgt
     1141 tgccacttca gcaggagtct tgatctttg
//
LOCUS       DAOQGJ010001697         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102012_length_1169_cov_3.491023, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001697 DAOQGJ010000000
VERSION     DAOQGJ010001697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102012_length_1169_cov_3.491023"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK683_20775"
     CDS             <1..526
                     /locus_tag="PK683_20775"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH10943.1"
                     /translation="FELQKYAEAAADFSESIKLDPSDINCFLKRGDAYLYMKEYEKAE
                     ADYTVSIEADPQHFPSYFARGNSRYSLNKLDDAINDFSKALDLCPELTDAVYWKARIL
                     QKKGDYKTAVKCFNEVLKARPDFVQAYYCRGECYSKLGNEDDAKQDHTKAYELNPSLT
                     NSLDGLDTLKAISF"
     gene            523..1164
                     /gene="lptE"
                     /locus_tag="PK683_20780"
     CDS             523..1164
                     /gene="lptE"
                     /locus_tag="PK683_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002155737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNH10944.1"
                     /translation="MRYKIMLKYFTIINIFLFYGCGNYLKDNALVRYFTKEPGRPPKV
                     KVKSADGKEETVQVPDRYRKVYVHNFQNNSYGPGLHVMLTQALKSEIDRRGRFIQTRD
                     KSEAMFRIYGSVTHYQKIGNIMDYANQQISSEITMLAKIELQDSAGEKIQLPRDEAMG
                     RAYYSEQIGYRESEEQAQLRMVNNLAMRLSEETEDGWYYHIVNTYYPPEEKKE"
BASE COUNT          399 a          213 c          266 g          291 t
ORIGIN      
        1 cttcgaactg cagaaatatg cagaagctgc agcggacttc agtgaatcca taaaacttga
       61 tccttccgac ataaactgtt tcctgaaaag aggagatgcg tatctgtata tgaaagaata
      121 tgagaaggca gaggcagatt atactgtttc cattgaagcg gatccacagc atttcccatc
      181 ctactttgca agaggaaaca gccgctattc tttgaataag ctggatgatg caatcaacga
      241 tttcagcaag gcactggatt tatgtcctga acttacggat gcggtttact ggaaagcaag
      301 aatcctgcag aaaaagggtg attataaaac ggcagtaaaa tgtttcaatg aggttctgaa
      361 agcaagaccg gactttgttc aggcttatta ctgtcgagga gaatgttatt ctaaacttgg
      421 aaatgaggat gacgcgaagc aggatcatac aaaagcttat gaactcaatc cctcgcttac
      481 aaacagtctt gacgggcttg acacactgaa agcaatttct ttttgagata taaaataatg
      541 ctgaaatact ttactataat caatattttt cttttttacg gatgcgggaa ttatctgaaa
      601 gacaatgctt tagtcagata ctttaccaag gaacctgggc ggcctcctaa agtaaaagtc
      661 aaatcagcag atggaaaaga ggaaactgtt caggttccgg acagatatcg aaaagtatat
      721 gtgcataatt tccagaacaa ttcctacggc cccggacttc atgtgatgct gactcaggcc
      781 ttgaaatcgg agatagacag aagaggacga tttattcaga ccagagacaa gtccgaggca
      841 atgttcagaa tttacggttc agtgactcat taccagaaaa taggaaacat tatggactat
      901 gcaaaccagc agatcagttc agagatcact atgctggcaa agatagaact tcaggattct
      961 gcaggagaga aaatacagct tcccagggat gaggcaatgg gaagggctta ttattctgag
     1021 cagattggat acagagagtc ggaagaacag gcacagctta gaatggtaaa caatcttgca
     1081 atgagacttt ctgaagaaac ggaagacggg tggtactatc atatcgtaaa cacttactat
     1141 cctcctgaag agaaaaaaga atgatggaa
//
LOCUS       DAOQGJ010001698         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102046_length_1169_cov_2.766607, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001698 DAOQGJ010000000
VERSION     DAOQGJ010001698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102046_length_1169_cov_2.766607"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..>1169
                     /locus_tag="PK683_20785"
     CDS             37..>1169
                     /locus_tag="PK683_20785"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH10945.1"
                     /translation="MLAFLYLFIGLYHFLLYVKRTKEKYNLFFAFLASAISFYTFART
                     NMVQDLRLDPNLIIKFEYPSLFTLAASSVLFTEDFILGRLTKFGKTYLGFSLFISLFT
                     LAVWDRIVYSYILQAWQMSVIFIFPYMIFRMVQGIFRKNMDAVRIFFGFITILAFATA
                     DLVGAMKLIPGFANPGLMKYGFLLYVSGIALVLANRFLRVHQEAEDLNLHLDEKVKEK
                     TAQLEKRTDELQQTLDEVRALKMQQDGDYFLTSLLLQPLMINFIKEGPVYIDFIIKQK
                     KTFEFKKKNYEIGGDICLADKIVLRKRNYSVFVNGDAMGKSIQGAGGALVLGVVFRAV
                     LSRTKRKDNAYLLPEIWLKECFQELHNVFVSFDGSMLISVVLGL"
BASE COUNT          311 a          232 c          263 g          363 t
ORIGIN      
        1 aggcagattg acagaagaac ggaaagggtg acgctgatgc ttgcgtttct ttatcttttc
       61 ataggacttt atcattttct tctctatgtc aaacggacca aggagaagta taatctgttt
      121 tttgccttcc ttgcgtccgc gatttccttc tatacatttg caagaacgaa tatggtacag
      181 gatctgagac tggatccgaa tctgatcata aagtttgaat atccttccct ttttactctg
      241 gctgcatctt ctgttctttt cactgaggac tttattctgg gaaggctgac aaagttcggc
      301 aaaacatact taggattctc tctcttcatc tcccttttca ctcttgctgt ctgggacagg
      361 atagtttatt cgtacatcct ccaagcctgg cagatgtctg tcattttcat tttcccttat
      421 atgattttca gaatggttca gggaatattc aggaaaaata tggatgcggt gagaatcttc
      481 ttcggattca tcaccattct ggcatttgcc actgcggatc tggtaggtgc aatgaaactt
      541 attcccggtt ttgcaaatcc aggcctgatg aaatacggat tcctgctcta tgtgtccggc
      601 attgcacttg tacttgccaa cagatttctg cgtgttcatc aggaagcaga ggatctgaat
      661 cttcatctgg atgaaaaagt aaaggaaaaa actgcgcagc tggaaaaaag aacggatgaa
      721 cttcagcaga ctcttgacga ggtcagggca ttaaagatgc agcaggatgg ggactatttc
      781 cttacatccc ttcttctgca gccgctgatg ataaacttca ttaaggaggg tcctgtttat
      841 atagatttta tcattaagca gaagaagaca ttcgagttta aaaagaagaa ttatgaaatc
      901 gggggtgata tctgtctcgc ggataaaata gtccttcgca agagaaatta ttccgttttt
      961 gtaaacggag acgcaatggg aaaatccatt cagggagcag gcggagctct tgttttagga
     1021 gttgttttcc gtgcagtgct ttcacgcaca aagagaaagg acaatgcata cctgcttcct
     1081 gaaatctggc tgaaggaatg ctttcaggaa ctgcacaatg tattcgtttc atttgacgga
     1141 tccatgctga tatcagttgt tctggggct
//
LOCUS       DAOQGJ010001699         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102133_length_1169_cov_1.772890, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001699 DAOQGJ010000000
VERSION     DAOQGJ010001699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102133_length_1169_cov_1.772890"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1169)
                     /locus_tag="PK683_20790"
     CDS             complement(<1..>1169)
                     /locus_tag="PK683_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10946.1"
                     /translation="VYADEPSVDETVMILRGLKNHYELHHGIRITDSAIIAAANLSSR
                     YITDRFLPDKAVDLIDEASSKMRIEIDSMPEELDRMMKKIQSLKIEREALKKEKDNAS
                     VQRLKELEKELQKQEEEFSILQAKWNSEKEKILKIKSLKEEIEKNRNLEKQAERDGDL
                     NKVAEIRYGKLIELNKQLQTANDELNLVSSSSRLLREEVTDEDIANIVSRWTGIPVSK
                     MLQGEKQKLLLMEGVLKKRVVGQDHVIQTICESIRRSRAGLSDPKKPTGVFLLLGPTG
                     VGKTETAKALAEFLFDDETAIVRIDMSEYMESHSVSKLIGAPPGYIGYDEGGQLTEAV
                     RRRPYSIVLFDEVEKAHKEVFNLFLQLFDEGRLTDSKGRTVDFRNTVIMMTSNIG"
BASE COUNT          259 a          305 c          220 g          385 t
ORIGIN      
        1 cacctatatt ggaagtcatc ataatgactg tgtttctgaa atcgactgtt cttcccttgc
       61 tgtcagtgag ccttccttca tcaaagagct gaaggaaaag attgaacact tccttgtggg
      121 ctttttccac ctcgtcaaaa agcacaatac tgtaaggtct tctgcggaca gcctcagtca
      181 gctgccctcc ttcatcgtat cctatgtatc ctggaggggc accgatgagt ttgctgactg
      241 aatgagattc catatactca ctcatatcga tccttacaat tgcagtctca tcatcaaaca
      301 gaaactctgc aagtgccttt gctgtttcag tctttcctac acctgtcggt ccaagaagaa
      361 gaaatactcc tgtaggtttt ttgggatcag agagccctgc acgcgatctt ctgatcgatt
      421 cgcagatcgt ctgaatcaca tgatcctgac ctaccactct tttcttgagc actccctcca
      481 tcaggaggag tttctgcttc tctccctgaa gcatcttgga aacaggaatt cctgtccatc
      541 tggatacaat gttggcaatg tcttcatccg tgacttcctc tctcagaaga cgcgatgagc
      601 tcgataccag attcagttca tcattggcag tctggagctg cttgttcagt tcaatgagtt
      661 ttccgtaacg gatttcagcc accttgttca gatcaccgtc tctttctgcc tgtttctcca
      721 gattccggtt cttttcaatt tcttccttca ggcttttgat tttcagtatc ttttcctttt
      781 cactgttcca ttttgcctga agaatggaaa actcctcttc ctgcttctga agttcttttt
      841 ccagttcctt cagtctctga acggatgcat tgtccttctc cttcttcagc gcttcccttt
      901 caatcttaag gctctggatt tttttcatca tccggtcaag ttcttccggc atactgtcta
      961 tttcgatcct catcttgctg gatgcctcat caatcagatc cactgccttg tcaggaagaa
     1021 atctgtctgt gatgtacctg gaagaaagat ttgcggctgc gatgatggca gaatcagtaa
     1081 tccgtatccc atgatgcagt tcataatgat tttttaaccc gcgaaggatc atcactgtct
     1141 cgtccactga aggctcatct gcataaaca
//
LOCUS       DAOQGJ010001700         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102192_length_1168_cov_3.358491, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001700 DAOQGJ010000000
VERSION     DAOQGJ010001700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102192_length_1168_cov_3.358491"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK683_20795"
     CDS             complement(<1..343)
                     /locus_tag="PK683_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10947.1"
                     /translation="MNEHIINERLPPSHKLPIDRLSNIFSDTVNSYKFYWFLSILNAL
                     KTDNSALKISIQYLCLEMMKQVWYPLEFFKLSFGTQDSFTKISKKIKLLKSDGKKASD
                     KSFDDQLKNYSE"
     gene            563..994
                     /locus_tag="PK683_20800"
     CDS             563..994
                     /locus_tag="PK683_20800"
                     /inference="COORDINATES: protein motif:HMM:NF021536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HNH10948.1"
                     /translation="MPVPAEYQRIYDHLYDFFKDVRDIAGIETTHRAYTTIQGVFQTF
                     RRRLEIKEAISFSNILPAGIRALFVAEWDTNEPKLPFSSRREMTLEVQSLRKEHNFAP
                     DSAISDVGRALRKNVDNQLLDEVLDRLSADARNFWNPEYAD"
     gene            984..>1168
                     /locus_tag="PK683_20805"
     CDS             984..>1168
                     /locus_tag="PK683_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10949.1"
                     /translation="MQTNYSPYAFGCLRLPSAAGGAFLAGSLPAVSQTLKLPQTLKLP
                     QTLKLPQTLKLPQTLKLP"
BASE COUNT          329 a          258 c          252 g          329 t
ORIGIN      
        1 cttcactgta atttttcagc tgatcatcaa aagatttatc cgaggccttt ttcccgtccg
       61 atttcagcaa tttgattttt ttagagattt tcgtaaaact gtcctgtgtt ccaaaagaca
      121 gcttgaaaaa ttccagagga taccaaacct gcttcatcat ctccaggcaa agatactgaa
      181 tggaaatctt cagtgcggaa ttatccgttt tgagagcatt cagaatggaa agaaaccagt
      241 agaatttata agaatttaca gtatcagaaa atatattgga taatctatcg ataggaagtt
      301 tatgcgaagg cggcagcctt tcatttatta tatgctcatt catttttaac ttccatacaa
      361 tttttcataa atatcatttc tgactgatgg catacgcgtc aacggaaaaa tatacctccg
      421 ctctgaaatc accgaaggtg aatgcagttc agaggtgcgt tccttgccgg ccttcgaaaa
      481 ctgcagtatt ggtccgataa cattcgagtt gcagaatacc gggatgctga aacagtagca
      541 gtatttcaag aggagtattc ttatgcctgt tcctgctgaa tatcagagaa tttatgatca
      601 tctttatgat tttttcaagg atgtcagaga catagccggt attgaaacaa cacaccgggc
      661 ttatacgaca attcaaggag tatttcagac cttcagaaga agactggaaa tcaaggaagc
      721 gatttccttt tcgaatatcc tgcccgcagg gatcagagcc ctctttgtcg ccgagtggga
      781 tacgaatgag cctaaacttc cattttcgag caggcgggaa atgaccctag aagttcagtc
      841 tctcagaaag gagcataatt ttgctcctga ttccgcgatc agcgatgtag gccgggcact
      901 tcgtaaaaat gttgacaatc agcttctgga tgaagttctc gaccgtttat cagcagatgc
      961 ccgtaatttc tggaatccgg agtatgcaga ctaattactc accttacgcc ttcggctgcc
     1021 ttcggctgcc ttcagctgca ggtggggcat ttttggcagg gagcctaccg gcggtttcgc
     1081 aaaccttgaa gctgccgcaa accttgaagc tgccgcaaac cttgaagctg ccgcaaacct
     1141 tgaagctgcc gcaaaccttg aagctgcc
//
LOCUS       DAOQGJ010001701         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102345_length_1167_cov_3.340827, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001701 DAOQGJ010000000
VERSION     DAOQGJ010001701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102345_length_1167_cov_3.340827"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1023)
                     /locus_tag="PK683_20810"
     CDS             complement(139..1023)
                     /locus_tag="PK683_20810"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNH10950.1"
                     /translation="MKAILKENSKVSLQDIPEPKIKEDEVLIRVHTACCCRTDSFAAQ
                     GKIRTANPLVLGHEFSGTVEEKGYEVRSVIVNERVAVMPIWKDSFGMYSGVMLGIDRN
                     GGFAEFASVPAECIHKIPDELSLKEAAYLEPVTAALAVIQADFSKKGKCVILGENRIS
                     DLIYRIMIFYGFQNITVLSAGSISAVSENEFDFGIETEAESEGFSHLIRAVRPRGKII
                     LKSRQPVPMQISVKNIAEKELVLQGAGYGSFEEAVKIAGSLKLSELMLDTAIEKIPEF
                     LLNTDMAGTEKKKICFSF"
BASE COUNT          287 a          268 c          238 g          374 t
ORIGIN      
        1 ctaggccggc cagaccgttt cgggaaatcc agatattttc tccgtccggt cccctgtgtc
       61 tcaatgaatg tactgatcgg agaagcagtt ccggattgat ttttttataa ggatgaaata
      121 ttgcagttat tccgcacatc agaaggaaaa acagattttc tttttttctg ttcccgccat
      181 atctgtattc agaagaaatt caggaatttt ttcaattgca gtgtccagca taagttcact
      241 caatttcaga ctcccggcaa ttttgactgc ttcttcaaaa cttccgtaac ctgctccctg
      301 aagaacaagt tccttctctg ctatgttctt tacggaaatc tgcataggaa ccggctgtct
      361 gcttttcaga atgatctttc ctctgggcct gactgctctg atcagatgag agaacccttc
      421 tgattcggcc tccgtttcaa tcccgaaatc gaattcattt tccgatacag ctgagatgct
      481 tccggcagaa agaactgtaa tattctgaaa tccataaaaa atcattattc tgtaaataag
      541 atcggatatt ctgttttctc ccagaatgac gcattttcct tttttggaaa aatcagcctg
      601 aatgactgcc agggcagctg tgacaggttc aagataggcc gcttccttca gactcaattc
      661 atcagggatc ttatgaatgc attctgccgg cacagatgca aattctgcaa atcctccgtt
      721 tctgtctatt ccaagcataa caccggagta cattccgaaa ctgtccttcc agatcggcat
      781 gactgcgacc ctttcattta caattacact tctgacctca tatcctttct cttccactgt
      841 ccccgaaaat tcatgaccga gcacaagtgg atttgcagtt ctgatttttc cctgagcggc
      901 aaaactgtct gttctgcagc agcaggcagt atggactctg atcagaacct catcttcttt
      961 tatttttggt tcaggtatat cttgcagaga tacttttgag ttttctttca gtattgcttt
     1021 cattttggtc aagaccagtg tttttcagtt ttctccaaaa taaagcttct tttggtagga
     1081 gttcctacgg atgaagggct gtgtgggggt gcaggagttc ctacggatga agggctgtgt
     1141 gggggtgcag gagttcctac ggatgaa
//
LOCUS       DAOQGJ010001702         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102357_length_1167_cov_3.061151, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001702 DAOQGJ010000000
VERSION     DAOQGJ010001702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102357_length_1167_cov_3.061151"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PK683_20815"
     CDS             complement(<1..1012)
                     /locus_tag="PK683_20815"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10951.1"
                     /translation="MKSDRALKSWIENDLQTKMVFLGGPRQVGKTTLAKSFTEKKEQY
                     LNWDFPDDRALIRQHRLNPELKLIVLDEVHKFAKWRTLVKGFYDKHGNTLSILVTGSA
                     RLDHFRKGGDSLQGRYHYYRLHPFSLAEADKKYKRQTVLDLMKFGGFPEPFLSRDERT
                     YRRWSRERIAKVIYEDLRDLENVKELSLLELLVDALPSKVGSLLSVNSLREDLEVSPN
                     TVASWIEILERLYYCYRISPMGAPKIRTVKKAGKLYLWDWAEVENQGARFENLVAGQL
                     LKYCHFLEDTEGYRMDLRFLRDTEGREIDFVVFRNKKALFAVECRTGEKHISPHIRYF
                     RSR"
BASE COUNT          313 a          273 c          238 g          343 t
ORIGIN      
        1 ttctgctgcg gaaatagcgg atatgaggag aaatatgctt ctctcctgtt ctgcattcga
       61 ctgcaaataa tgctttttta ttgcggaata cgacaaagtc aatttctctt ccttcagtat
      121 cccgcagaaa ccgcagatcc atcctgtatc cttccgtatc ttccaaaaaa tgacagtatt
      181 tcagaagctg tcctgcgaca aggttttcaa atcgtgctcc ctgattttct acttcagccc
      241 agtcccacag ataaagtttg ccagcttttt ttactgtccg aatctttggt gcacccattg
      301 gagaaatacg ataacagtag tacagccttt ccagaatctc aatccagctc gcgaccgtgt
      361 tcggtgatac ttccaagtcc tctctcaggg agttgacaga aagaagtgag cctactttgg
      421 aaggcagtgc atccacgagg agttccagaa gcgaaagttc ttttacattt tccagatctc
      481 tcagatcttc ataaataact ttagcaattc tttctctgct ccatctcctg taagttcttt
      541 catcacggga cagaaatggt tccgggaatc ctccgaattt cataagatcc aggactgtct
      601 gtcttttata ttttttatct gcctctgcga gcgaaaacgg atgcagacgg taataatggt
      661 acctcccttg cagagaatct cctcccttcc gaaaatgatc gagtcgtgca gatcctgtca
      721 caagaataga gagtgtgttg ccgtgtttgt cgtagaatcc cttgaccagt gtcctccatt
      781 tggcaaattt atgaacttca tccagaacaa tcagtttcag ttcaggattg agtctgtgct
      841 gccggatcaa tgctctgtcg tccgggaaat cccagttcag atactgctct tttttctccg
      901 taaaagactt ggcaagcgta gttttgccta cctgcctcgg tccgccgaga aacaccattt
      961 tggtctgcag atcattttca atccaggatt tcagagcacg gtcagatttc atactactga
     1021 aaaatacacg tgttcatttt tcagtcaagt gaaaaatgga ggcgggaatg gggatttaac
     1081 ctcccgctcg cagggaatag ggaatgaaaa aaatttcata gtcgggaaaa aaagcagaaa
     1141 aaaacagagg gaatctcctc tttaatt
//
LOCUS       DAOQGJ010001703         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102379_length_1167_cov_2.599820, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001703 DAOQGJ010000000
VERSION     DAOQGJ010001703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102379_length_1167_cov_2.599820"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PK683_20820"
     CDS             complement(<1..497)
                     /locus_tag="PK683_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10952.1"
                     /translation="MMLFFSKIFSSFIAVSILFCFCIKSGISAEEKTGDPTGNADKWD
                     RMIDEYTSESLLEKRKRKDTEENRYSTRLLSLNPGISFSHITSSVRRNDYTSLRTHYP
                     FFQPNLALDIKSKEFRISENFGFLILNQSMFYRLNNQETDPIPDNVSVNNFGQMNFIN
                     SDLKN"
     gene            786..>1167
                     /locus_tag="PK683_20825"
     CDS             786..>1167
                     /locus_tag="PK683_20825"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH10953.1"
                     /translation="MKDYFRNKGSEIDMSSLMDIVFILLIFVMLSISFQKKIVTMDLD
                     LPKSENQDAGNSKPKKEIIVLKTGEILYAGRKTDLKNLETEVRKNPKTEEYILLTEKQ
                     AEFEKFLKVLEVLKRSGSDKIDLGL"
BASE COUNT          394 a          209 c          238 g          326 t
ORIGIN      
        1 ctgttcttca aatccgaatt gataaagttc atctgaccga aattgttcac agatacattg
       61 tcggggatcg gatcagtttc ctggttgttc agtctgtaga acatactttg gttcaggatc
      121 agaaaaccga agttttccga tatcctgaat tcctttgact tgatgtccaa agcaagattg
      181 ggctggaaaa aaggataatg cgttctgagt gaggtgtaat cgtttcttct tacagaggaa
      241 gtgatatgcg agaaagaaat acctgggtta agggacagta aacgagttga atatctgttt
      301 tcctctgtat cttttctttt acgtttttcc aaaagactct ctgacgtata ttcatcgatc
      361 attctgtccc atttatcagc atttccggtc ggatctcccg tcttttcctc ggcagagata
      421 ccgcttttaa tgcagaaaca aaacagaatg gaaacagcaa tgaaggaaga aaagattttt
      481 gagaaaaaca gcatcatttt gagcagttcc gaacattgag cggatctgca tcaaatctgc
      541 aggcaaatat cccagtttcc tgcagtgtgc ttccgataag aaatcgaaga agcacaaatt
      601 ggggaatgat taacgattca tgataaatca ttgaaaaatt ttacatatct catctttttg
      661 gcaggagcag ggtctcatga ataaaccaac ctcagtattc catttcacca agtgtcagaa
      721 ataactgaca aggtgtttta ctcaactaca aattctccta tgtcgaaagc aggaaaacaa
      781 aactgatgaa agactacttc aggaataaag gatcagaaat tgatatgtcc agtctgatgg
      841 acatagtgtt catacttctt atctttgtaa tgctcagcat cagctttcag aaaaagattg
      901 taactatgga tctggacctt ccaaaatcag aaaatcagga tgcaggaaat tcaaagccga
      961 aaaaggaaat cattgttttg aaaactggag aaatccttta tgcaggcaga aaaacggatc
     1021 tcaagaatct ggaaacggaa gtcagaaaaa acccgaaaac tgaagaatac attctgttaa
     1081 cggaaaagca ggcagaattt gaaaagtttc tcaaggtact tgaggtattg aaaagatcgg
     1141 gatctgacaa aatagatctt ggactga
//
LOCUS       DAOQGJ010001704         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102388_length_1167_cov_2.484712, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001704 DAOQGJ010000000
VERSION     DAOQGJ010001704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102388_length_1167_cov_2.484712"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PK683_20830"
     CDS             <1..305
                     /locus_tag="PK683_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNH10954.1"
                     /translation="SLYRASATIVLSLFVIFSAIFLMLIFDPKLSFLDICFEAVSAFG
                     TVGLSKGITSNLSTASKSVLCFVMFAGRVGILTILIALLPKRKSLKYSYIKEYVVI"
     gene            428..>1167
                     /gene="tpiA"
                     /locus_tag="PK683_20835"
     CDS             428..>1167
                     /gene="tpiA"
                     /locus_tag="PK683_20835"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNH10955.1"
                     /translation="MRRTIIAGNWKMNLDKKNMEDLVTGIKKGLETKKTDHSILVFPS
                     AVHLAYVSEQFKGTKVETGVQNIYPSGLAAYTGETSVDQIKDFGIKTVLLGHSERRQF
                     LGETDEFLNQKVKFCLEKGFLPMLCVGELLSEREANATMKVLERQIREGLKGVSSADS
                     SKVCIAYEPVWAIGTGKVATPEQAEEAHAFIRSELAKLFGKNESEEMSILYGGSVKPD
                     NVKTLLEKPNVDGGLVGGASQKADSFLA"
BASE COUNT          364 a          238 c          263 g          302 t
ORIGIN      
        1 gttccctgta ccgtgcatcg gcaaccatcg tgctttcact ctttgtgata ttctctgcca
       61 ttttccttat gctgatcttt gatcccaaac tgtcctttct ggatatctgt tttgaagctg
      121 tttccgcttt cggaaccgtc ggtctttcca aaggaatcac ttccaatctg agcactgcaa
      181 gcaaatcagt tctttgcttc gtaatgtttg caggacgtgt aggaattctg acgatactca
      241 ttgcactgct ccctaaaaga aaatccctta aatacagtta tataaaggaa tatgttgtca
      301 tctgaacaaa aacacttgtg ctgaccgtgt atttttgaaa tatagggatt cttgcagata
      361 aatttctgca aagaagcaga tcggtatgca ctcacggctg actgcatctg ttaaaacagg
      421 agaacttatg agaagaacaa tcattgccgg aaactggaaa atgaatctgg acaaaaaaaa
      481 tatggaagat cttgtaactg gaatcaaaaa gggtctggaa acaaagaaaa cagaccacag
      541 cattctggtt ttcccttcag cagttcatct tgcatatgtt tcagaacagt tcaaaggcac
      601 aaaagtggaa acaggagttc agaatattta tccgtccggg cttgcagcat acactggaga
      661 aacctctgtt gatcagatca aagactttgg aatcaaaact gttctgctcg gccattctga
      721 aagaagacag ttcctcggtg aaactgacga gtttttgaac cagaaagtaa aattctgtct
      781 ggaaaaagga tttctcccga tgctctgcgt aggagaactg ctttcagaga gagaggcaaa
      841 tgcgacaatg aaggtactgg aaagacagat acgcgaaggt ctgaaagggg tcagcagtgc
      901 cgacagctcc aaggtttgca tagcatatga acctgtatgg gcaatcggaa caggaaaagt
      961 ggctacaccg gaacaggcag aggaagctca cgcattcatc agaagcgagc tcgcaaaact
     1021 tttcggaaaa aatgaatcag aagaaatgtc catcctttac ggcggttcag tcaagccgga
     1081 caatgtaaaa actcttctgg aaaaaccgaa tgtggatgga ggtcttgtag gaggagccag
     1141 ccagaaagca gattcttttc tagcttt
//
LOCUS       DAOQGJ010001705         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102500_length_1166_cov_3.083708, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001705 DAOQGJ010000000
VERSION     DAOQGJ010001705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102500_length_1166_cov_3.083708"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PK683_20840"
     CDS             complement(<1..635)
                     /locus_tag="PK683_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10956.1"
                     /translation="MILKTDSVLTEESVFRISFFRSYMKNITAYFILLFSSYSVFPDS
                     KPVNLMYRNSGFTAESYLKKTVCTKKSNCSPYILPKGKNDPRFISIHKRISGTDQSRR
                     VIQLGSGDSLQYRLFFPLNHSLRLQSEYGETEVLLNGESAKDGRLVSKKNYNIITVRA
                     KKETLITEALLLPEKREKQPDVLFIVIDSLRGDVPGFNGGKFGVTPKMDEIS"
     gene            complement(654..>1166)
                     /gene="metG"
                     /locus_tag="PK683_20845"
     CDS             complement(654..>1166)
                     /gene="metG"
                     /locus_tag="PK683_20845"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNH10957.1"
                     /translation="ENIGRIIENRKVEAYEMLSKRVDTKAIELMTEESRIKEEPKEQK
                     SAEKKPAETKEAGKVSESGFIKIDDLAKVELRVGLITEASAVEGADKLVNVKVDLGEK
                     GIKNVFAGIKAAYSPEELKGMKVVVVANLEPRKMKFGVSEAMLLASGKENTLSLFVPH
                     RNAEPGDLLK"
BASE COUNT          271 a          270 c          246 g          379 t
ORIGIN      
        1 gagatctcat ccattttagg agtgacgccg aatttcccgc cgttgaatcc ggggacatct
       61 cctctcaggg aatcaatgac gatgaaaagt acatcaggct gcttctcccg tttttcaggg
      121 agcagaagag cttcagtgat cagtgtttct ttttttgcac ggactgtaat gatattataa
      181 tttttttttg aaacaagtct tccatccttt gcggattccc cgttcagaag aacctccgtt
      241 tctccgtact cagactgaag cctgagactg tgattcagag gaaaaaagag cctgtactgc
      301 agagaatcgc ctgagcctag ctgaatcact cttctggact gatctgttcc gctgattctt
      361 ttatgaatgc tgatgaagcg gggatcattt ttcccctttg gcaggatata gggactgcag
      421 tttgattttt ttgtgcatac agtctttttg agatagcttt ctgcagtaaa gccgctgttt
      481 ctgtacatca gatttacggg ttttgagtca gggaatacgg aataggagga aaacagtaaa
      541 atgaaatatg ctgtaatgtt tttcatatat gaacggaaga aagaaattct gaaaacagac
      601 tcctcggtca ggacggaatc tgtcttcaga atcactgtat tttcagaaaa tgcttacttg
      661 aggagatccc caggctctgc atttctgtgg ggcacaaaaa ggctcagagt attctccttg
      721 ccggatgcca gaagcatcgc ttcggaaacc ccgaatttca tcttcctggg ctccaagttg
      781 gcaacgacga ctaccttcat ccccttcagt tcttcaggac tgtatgcggc cttgattccg
      841 gcgaatacat tcttgattcc cttttctcca agatccactt ttacattgac aagcttgtca
      901 gcaccttcca ccgccgaagc ttctgtgatg agtccgacgc gcagttctac ttttgcgaga
      961 tcatcgatct tgatgaaacc tgattcagaa acctttcctg cttcctttgt ttctgcaggc
     1021 ttcttttcgg cggacttctg ttccttgggt tcttccttga ttctgctttc ttctgtcata
     1081 agttcaattg cctttgtatc cactcttttg gagagcattt cgtatgcttc cacttttcta
     1141 ttttcaataa ttctgccgat gttctc
//
LOCUS       DAOQGJ010001706         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102535_length_1166_cov_2.552655, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001706 DAOQGJ010000000
VERSION     DAOQGJ010001706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102535_length_1166_cov_2.552655"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PK683_20850"
     CDS             complement(<1..919)
                     /locus_tag="PK683_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10958.1"
                     /translation="MQNISELLLNTLRQNTLTALFLLSFLLFKGADAETSQENKFKAF
                     VSNPNGMFLYADYSDKKNRIFAPYQTELSKSEETVSGKNDSETFCKYEFNNKIYYNSC
                     DENPVLEFRKPEGESFTYPHSAKVSIYRIPFQNQEVISEIPEFSMMKIHSVSEEWYEA
                     EFNGKKGCIRKENCIEAAPSDQVKFLLELKEKETDGYFVVSSSKPVFFPHNEQDRSTS
                     KKSTEEILRGKPISDIAEYLEGSNQEMKKVQTGKGTALKSIKTIVLKGSRYYMLTYPS
                     GYDLKYLWISEKDGKFLSKNEYSEYTFSKS"
BASE COUNT          312 a          217 c          233 g          404 t
ORIGIN      
        1 tcgacttcga aaaggtatat tccgagtatt catttttcga taaaaatttt ccatcctttt
       61 ctgaaatcca gagatacttc agatcgtaac cggacggata agtgagcata taatatcggg
      121 atcctttcaa cactattgtt tttatggatt tcagagctgt tccttttccc gtctgaactt
      181 tcttcatttc ctgattcgat ccttccaggt attctgcaat gtcggaaata ggttttcctc
      241 tcaatatttc ctcagtgctc ttttttgatg tactgcggtc ctgttcattg tgcggaaaaa
      301 aaaccggctt tgaggaagag acgacgaaat aaccgtctgt ttccttttct ttcagctcaa
      361 ggagaaactt tacctgatca gatggtgccg cttcaataca gttttctttg cggatgcagc
      421 cttttttccc attgaattct gcttcgtacc attcctcact gactgaatga attttcatca
      481 ttgaaaattc aggaatctcg gatatgactt cctggttctg aaaagggatt ctgtaaatgg
      541 agacttttgc agaatgaggg taagtaaaac tctcaccttc aggttttctg aattcaagga
      601 caggattttc atcacaggaa ttataatata ttttattatt gaactcatat ttgcagaaag
      661 tttcggaatc attttttcct gagacggtct cttccgattt ggacagttca gtctgataag
      721 gcgcaaatat tctgttcttt ttgtcgctgt agtctgcata cagaaacatt ccattgggat
      781 tgctgacaaa tgctttgaat ttattttcct gggaagtctc ggcatctgcg cctttgaaaa
      841 gaaggaagga aagcaggaaa agcgctgtaa gcgtgttttg ccgaagggta ttcagtaaaa
      901 gttccgatat gttctgcatt ttatcattgt tccgtaggga tatatgcttt tgaagaactt
      961 ttttccgtgg gcaattattt tttgaaagcg gatttcctga atcagtgaga cttcgtcggg
     1021 cagcgtcatt tcaaaaacca ttttttggag taataaccgc ttaaaaaaaa gttttgtttc
     1081 acagacagag taaaattctg gacatatgag aattctgata ctgatttcca tgcttttttc
     1141 cggatgtact ggcgggcata aaaaaa
//
LOCUS       DAOQGJ010001707         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102765_length_1165_cov_1.719820, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001707 DAOQGJ010000000
VERSION     DAOQGJ010001707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102765_length_1165_cov_1.719820"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..>1165)
                     /locus_tag="PK683_20855"
     CDS             complement(289..>1165)
                     /locus_tag="PK683_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10959.1"
                     /translation="SVPTLLLILLMREGVQMKEDGSIVADTSVSSGKGTFSEILPAVK
                     KTGVDTFIIMKNVFTERPFWVYLFMLGILVFIRLVFYHFHYTFPKYGIRILGEGVKIG
                     NIYGILNPVLIIFLVPVIAHLTKEVRSYTMLTFGTAVSALSIFIAALPPSLFSPFTKT
                     WIGDLIFIRWLELGPDKENPIIFGLILFVIIFTIGEAIWSPRLFQFSAEIAPEGKEGT
                     YLALSYLPYFLAKMIAGPLSGWLISNYAPANASSFPHQHMIWIWIGIMSAFSPIGLLI
                     FRKTFQKAENRKDSE"
BASE COUNT          361 a          246 c          235 g          323 t
ORIGIN      
        1 gtaccctcat cacttaaagt atttcagtat ctctcctgta atttccagac cggctgaaac
       61 tgttttatca tgaaaaagta ttcttcctgt tctgtctttc agaacggtgt ctatctctgc
      121 acctatgccg caaaagatga taaaggaaac ttataatatg cagtttttga ttctgatccg
      181 taaatagcct gaataaatga atgagaatcg tctcccatta agcatatttt tcctttctgc
      241 tgtacagaaa gcagtgattg tttactcttg cccagattag aatagcagtc actcagaatc
      301 ctttctgttt tcagccttct ggaatgtttt tctgaatatc aaaagtccga tcggagaaaa
      361 agcggacatt attccgatcc atatccagat catatgctga tgaggaaaag aagaagcatt
      421 cgcaggtgca taattagaaa tcagccagcc ggagagagga cctgcaatca tctttgcaag
      481 aaagtagggc agataggaaa gcgcgaggta agtgccctcc tttccttcgg gtgcaatctc
      541 tgctgaaaac tggaagagcc tcggcgacca tattgcctct cctattgtaa atatgatcac
      601 aaataatata agtccaaata taatcggatt ctctttgtcc ggaccaagtt ccagccagcg
      661 gataaaaatc agatccccga tccaggtttt tgtgaacgga gaaaacagtg aaggggggag
      721 agcggcgatg aaaatagaaa gagctgaaac tgctgttccg aaagtcagca tcgtatagga
      781 tcgaacttcc ttggtcagat gggcaatgac cggaaccagg aaaataatca gcacaggatt
      841 cagtattccg tatatatttc cgattttcac cccttcgcca agaattctga ttccgtactt
      901 tggaaaggta taatggaaat gataaaaaac aagtctgata aagaccagta ttccaagcat
      961 aaaaagatat acccagaaag gtctttctgt gaatacattt ttcataatga taaatgtatc
     1021 aactcccgtc ttttttacgg ccggaaggat ttctgaaaaa gttcctttgc ctgaactgac
     1081 ggatgtatcc gcaactatgc ttccatcctc tttcatctgc actccctctc tcatcagcag
     1141 aatcagaaga agggtcggaa cagaa
//
LOCUS       DAOQGJ010001708         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102773_length_1165_cov_1.609910, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001708 DAOQGJ010000000
VERSION     DAOQGJ010001708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102773_length_1165_cov_1.609910"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PK683_20860"
     CDS             complement(<1..378)
                     /locus_tag="PK683_20860"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNH10960.1"
                     /translation="MKETETIKNRVLSELEDAFSIYISEDRPELKGSDFRIRLEYSRN
                     EKFGDYSSSAALENKNLLGNPVHAAEAIVKRLKNRSFFSDVNFTPPGFINFRISETAL
                     SEYLKGILQNEDFRFPKTGNPEKI"
     gene            complement(375..1136)
                     /gene="recO"
                     /locus_tag="PK683_20865"
     CDS             complement(375..1136)
                     /gene="recO"
                     /locus_tag="PK683_20865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNH10961.1"
                     /translation="MSLKKESGIVISVRPSGDSDSFLDLLTEKGSRLHLIYKGIRKSR
                     KRSLLAAELGSKIQADYYESQGRQFYHIKEASLIERYDNLKKDYLGYLVLNSIFEILT
                     ALYPKGETDEKIFQFLSAVLSKFNESGIHSVGILSFKTRLLHLLGYLPSVFVCQNCGA
                     DLFEFQEIFLDRYQFGMYCQECHPEKNEPTAVLKLYHRILRTNYETLISLDNQLRTVA
                     RADFLLNQYINSISHRELKSPELLYSSLREQKLDV"
BASE COUNT          318 a          252 c          231 g          364 t
ORIGIN      
        1 aatcttttcc ggatttccgg ttttgggaaa tcggaaatcc tcattctgaa gaatcccttt
       61 gaggtactcg gagagtgctg tttctgaaat tcggaaattg atgaatccgg gaggagtgaa
      121 atttacatct gaaaaaaatg aacggttctt cagacgtttt acaattgctt ccgctgcatg
      181 aacaggattt cccagcagat tcttgttttc aagagccgct gaacttgaat agtctccgaa
      241 cttttcattt ctggaatact ccagcctgat cctgaaatct gatcctttca gttcaggtct
      301 gtcttcggaa atataaatgg aaaaagcatc ttccagctcc gaaagcactc tgtttttgat
      361 cgtttccgtt tccttcatac gtccaatttt tgctccctca gactggagta aagcagttca
      421 ggacttttta attccctgtg cgaaattgaa tttatgtact gattcagaag aaaatctgct
      481 cttgcaacag ttctgagctg attgtcgaga gagatcagcg tttcataatt tgttctgagt
      541 attctatggt acagtttcaa taccgcagtc ggctcatttt tctcaggatg gcattcctga
      601 cagtacattc cgaactgata cctgtccaaa aatatctcct gaaactcaaa aagatctgcc
      661 ccgcagttct gacagacaaa caccgaggga agatacccta gaagatgaag gagcctagtc
      721 ttgaatgaaa gaattcccac cgaatgaatg ccggactcgt tgaatttgga aagaactgcg
      781 gacagaaact gaaaaatctt ttcgtcagtc tcacctttcg ggtaaagggc tgtcagtatt
      841 tcaaagatgc tgttcagcac taaataacca agataatctt ttttcagatt atcatatctt
      901 tcaatgaggc ttgcttcctt gatatggtaa aactgccgtc cctgcgattc atagtagtca
      961 gcctgaatct ttgaaccgag ctctgctgca agcagacttc tttttctgct ctttcggatg
     1021 cccttgtaaa tcagatgaag cctgcttcct ttttcagtca gcaggtcaag aaaggaatca
     1081 gaatctcctg acggtcttac cgatatgacg attccagatt cctttttaag ggacattact
     1141 ctcctttaaa gaaaggtttt ctttt
//
LOCUS       DAOQGJ010001709         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_102876_length_1164_cov_2.522994, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001709 DAOQGJ010000000
VERSION     DAOQGJ010001709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102876_length_1164_cov_2.522994"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PK683_20870"
     CDS             complement(<1..531)
                     /locus_tag="PK683_20870"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNH10962.1"
                     /translation="MNTERLVSLFPVWLLIACGVSFCRPAIFTWLGKDLIPLALGIVM
                     LGMGLTLKLEDFRRVSEYPKPVLTGVILQYTVMPLAGTFIAYVSGLNNEFAAGLILVS
                     CCPGGTASNVLTYIAKGNVALSVTMTVISTLMAVIATPYLTLLLAGNRVEVNALGLVL
                     STFQVIIVPVILGAFLS"
     gene            complement(528..>1164)
                     /locus_tag="PK683_20875"
     CDS             complement(528..>1164)
                     /locus_tag="PK683_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10963.1"
                     /translation="QFIAVTGLTKPGAHSWADLFFLCKFRTQTKINTDMFIFSPPVAG
                     ALLFGVGLFLFLKFFFKLDKVFYKPFFTLLLIIIGLNIIISDLKFGMGSDSNTAIFKE
                     SSLTVTDPSAEYNVMFTKSTTDFTGLAAGGGNHLVDSNTVFAKNTIIIRADVPTRILI
                     YPTLGVVKLPNGMKILPLFKNVYNNKSFTAESRAITIKVSIVFGAVDIVEQ"
BASE COUNT          343 a          245 c          269 g          307 t
ORIGIN      
        1 ggaaagaaag gctccaagaa tcaccggaac aataatgacc tgaaaagtag aaagaaccag
       61 acccaatgca ttgacttcta ccctgtttcc tgccagaagc agggtcagat atggggttgc
      121 aatgactgcc atcagcgtgg aaatgactgt catcgtaacg gacagtgcta cattgccttt
      181 tgcaatgtaa gtcagtacat tggaagccgt tccgcccgga cagcaggaaa ctaaaatcag
      241 tcctgcagca aattcattgt tcagtccgga aacataggcg atgaaagttc ctgccaaggg
      301 cattacagtg tactgaagaa ttacaccggt cagtaccggt tttggatatt cggacactct
      361 tctgaaatct tccagcttca gggtgagtcc catcccgagc attacaattc ccagtgcaag
      421 tggaatgaga tcctttccaa gccaggtgaa tatcgccggc ctgcagaatg aaacaccgca
      481 ggcaatcagg agccagacag ggaaaagact gaccaacctt tcagtattca ctgttctacg
      541 atatccactg caccgaatac aatgctgact ttgattgtaa ttgccctgga ttcagccgtg
      601 aaggatttat tgttgtatac gtttttgaaa agaggaagga tcttcattcc gttcggaagt
      661 ttgactactc caagcgtagg gtagatcagt attctggtcg gtacatctgc cctgataatg
      721 attgtgtttt tggcaaaaac tgtattcgag tctaccagat gatttccgcc tccggcagca
      781 aggccagtaa aatcagttgt gctttttgta aacattacat tgtattctgc agaaggatca
      841 gtgactgtca gagaggattc cttgaaaatt gctgtatttg agtcagaacc cattccgaat
      901 ttgagatcgc tgataatgat gttcagccct atgatgatta gaagcagagt aaagaacggt
      961 ttgtaaaata ccttatccag tttgaagaaa aacttcagga aaaggaacag tcctactccg
     1021 aacagaagtg ctccggctac cggcggtgaa aatataaaca tatctgtatt gattttcgtc
     1081 tgagttctga atttgcataa gaaaaatagg tctgcccatg aatgagctcc tggtttggtc
     1141 agaccggtga ctgctataaa ttgg
//
LOCUS       DAOQGJ010001710         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103121_length_1162_cov_3.559169, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001710 DAOQGJ010000000
VERSION     DAOQGJ010001710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103121_length_1162_cov_3.559169"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PK683_20880"
     CDS             complement(<1..379)
                     /locus_tag="PK683_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10964.1"
                     /translation="MENSRKWKDFILYSFQGHIESRWNANHGRVTAYAWWATLWENVQ
                     AGKEAETYKELNADTSLLNEFDYIGVVLGEGYTLIEKWARDSSETAPISLKKYLFHKK
                     SYNKGDFNTISEINGKYYMMTVSA"
     gene            complement(480..1055)
                     /locus_tag="PK683_20885"
     CDS             complement(480..1055)
                     /locus_tag="PK683_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10965.1"
                     /translation="MKYLILTSVLLTWFCAGAPEVKEEDVTLRPGETRKILSEKEKIQ
                     GTTFSKGTELILNKNGYVNRAVLGDSQKILDEKYSGSFQHIFPAGSIILYHPETNTFG
                     DRSKTPEIPKSIMLRKTAEIMGHSFPPGTVFILNIKNSMIRTNKEYYPNIGVENTFFE
                     IKNEGKVIPAGSNIVFEDQKTVKVRNKDKWE"
BASE COUNT          294 a          238 c          225 g          405 t
ORIGIN      
        1 gggctgatac ggtcatcata taatactttc cgttaatttc ggaaattgta ttaaaatccc
       61 ctttattgta acttttctta tgaaacagat attttttgag cgagattgga gctgtttctg
      121 aagagtctct tgcccatttt tcaatcagag tgtagccttc gccgaggact accccaatat
      181 agtcaaattc gttcagcagg gaagtgtctg cattcagttc cttgtatgtt tctgcttctt
      241 ttcctgcctg gacattttcc cacagcgtcg cccaccaggc ataagccgtg actctgccat
      301 gatttgcatt ccatctgctt tcaatatggc cctggaatga gtacagaatg aaatccttcc
      361 actttctgct gttttccata ttcgcattca ggtatatgca gaattcggaa aaagaaacca
      421 gcagaaacag tgttggaaga aatttattta tccaagtctt aagtttatcc aaggataaat
      481 tattcccatt tgtccttgtt tcttactttg actgttttct gatcttcaaa aacaatattg
      541 gagccggctg gaatgacttt cccttcattt ttgatttcaa aaaaagtatt ttccacaccg
      601 atattcggat agtattcctt gtttgtcctg atcatactgt tcttgatatt caaaatgaat
      661 acagttccgg gagggaaact gtgtcccata atttcagcag tcttgcggag cataattgac
      721 tttggaattt caggtgtctt gcttctgtct ccgaaggtat ttgtttcagg atgatacagt
      781 atgatggaac cggctggaaa aatgtgctga aaactccctg aatatttctc atccagaatt
      841 ttctgactgt ctccgagaac cgcacggttg acatatccgt ttttgttcag tatgagttct
      901 gtgccttttg aaaatgttgt gccctgaatt ttttcttttt cggagagaat ttttctcgtt
      961 tcacctgggc gaagagtgac gtcctcttcc tttacttccg gtgctcctgc acagaaccag
     1021 gtcagaagga ctgacgtgag tattagatat ttcatagatt ctccttggag tccagaggaa
     1081 tcagaactgt aaaaactgta ctccctggaa ctgattcaac tgaaattttt gctttatgct
     1141 tttcaagaat atttttgacg at
//
LOCUS       DAOQGJ010001711         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103320_length_1161_cov_3.275769, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001711 DAOQGJ010000000
VERSION     DAOQGJ010001711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103320_length_1161_cov_3.275769"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PK683_20890"
     CDS             <1..602
                     /locus_tag="PK683_20890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNH10966.1"
                     /translation="IRVEASAMASMDTNVTMKTRFKGGFKRFLSGESLFINEFTAENG
                     EGEINIAPGAPGDLEHVYLEDQVIYLQGSGFVASGMDVETDAKWQGFKKGFFSGQSMF
                     LIRCSGKGDLWFNTYGGLIEIDVDGDYVVDTGYIVAFTEGLEYDVRAVGGYKSLFFSG
                     EGLVCRFQGKGKVWIQTRQSSALASFLYPFRPIEKKTSG"
     gene            637..1005
                     /locus_tag="PK683_20895"
     CDS             637..1005
                     /locus_tag="PK683_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10967.1"
                     /translation="MVCKVCAYSAKGGFAKPSGGPNLTPPPAPLSFASKAKERGRGGR
                     FYAVLRLPMNLQELSERYICSKIITQTVLKAGWGESQIETESHYPDLLLEKYKSLLSE
                     IEVTENELKTELASALSHHF"
BASE COUNT          356 a          224 c          288 g          293 t
ORIGIN      
        1 cgatcagagt ggaagcgtca gctatggcgt cgatggatac caacgtcaca atgaagacaa
       61 ggttcaaggg cggattcaaa agatttcttt caggagagtc tctgttcatc aatgagttca
      121 ctgcagagaa cggagaaggg gaaatcaata ttgcacctgg agcacctgga gatctcgagc
      181 atgtgtatct cgaagatcag gtgatctatc ttcagggttc aggatttgtc gcttccggaa
      241 tggatgtgga aactgatgca aaatggcagg gattcaaaaa aggattcttt tcaggtcagt
      301 ctatgtttct catccggtgc tcaggaaagg gagatctctg gttcaataca tacggtggac
      361 tgattgagat tgatgtggac ggggactatg ttgtagacac cggctatatc gttgcattca
      421 ctgaaggact ggagtatgat gtccgtgcag tcggcggata caaatcactt ttcttctcag
      481 gagagggact ggtatgccgt tttcagggaa agggaaaagt ctggattcag acaagacagt
      541 catccgcact tgcttccttc ctgtatcctt tccgtccgat cgaaaagaaa acctcgggct
      601 gacaaccttt tgcaaagcca tcaggtgggc aaaaggttgg tctgcaaggt ttgcgcttac
      661 agcgcaaagg gcggttttgc aaagccatca ggcgggccta acctcacccc gcctccggca
      721 cccctctcct ttgcaagtaa agcaaaggag aggggtcgag gaggacgctt ttacgcagtc
      781 ttgcggcttc ctatgaactt acaagaactg agtgaaagat atatttgctc aaaaatcatt
      841 acacagacag ttttaaaagc tggatggggt gaaagtcaaa ttgaaaccga atcccactat
      901 cctgacttgc tccttgaaaa atataaatct ttattatctg aaattgaagt aaccgaaaat
      961 gaattgaaga cagaactggc aagtgcgctt tcgcatcatt tttaaaaaga attaaatgaa
     1021 gagaggaaat atgaatcata agtcgtccat tgacctgata atagaggaat acaagaaaaa
     1081 tgtagataaa actcttttgg cagaaaattt aaaattatca tataccgaga gagcagaaaa
     1141 attgcaggat atgcttagag c
//
LOCUS       DAOQGJ010001712         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103405_length_1161_cov_2.032550, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001712 DAOQGJ010000000
VERSION     DAOQGJ010001712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103405_length_1161_cov_2.032550"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PK683_20900"
     CDS             <1..517
                     /locus_tag="PK683_20900"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH10968.1"
                     /translation="VVITGSESTGKTTLCRKLAEYFHTEWVPEYGRIYLENKGSEMEK
                     SDLEIIGKGQAELEDRLSLSAEKVLICDTDLIITEMYSEWYYSFCPESVRKEIIRRKY
                     DLHILLEPDVPWVKDGLRDFPDRRIEFRDELLRKLAEYGCRTVRVSGNYEDRFEKTVK
                     LILDILSSKKE"
     gene            563..895
                     /locus_tag="PK683_20905"
     CDS             563..895
                     /locus_tag="PK683_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10969.1"
                     /translation="MLRRILRIVSLFILSVFLCISPMSDRLEQQDEIIRPVHPVMENI
                     DFTMFSDYFPEYRRLSQDSFLKLVLEKKFSFLMSLSTKIPFQQFSKAFLFSSQILLYS
                     LISLPPPV"
     gene            943..>1161
                     /locus_tag="PK683_20910"
     CDS             943..>1161
                     /locus_tag="PK683_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="channel protein TolC"
                     /protein_id="HNH10970.1"
                     /translation="MHLYVLIFLTASAFSLISAQEKNPKDSGSSAKVVRMKLQDAEEK
                     FLKNNLQLLAQKFQIDGSRARILQARLYA"
BASE COUNT          356 a          229 c          246 g          330 t
ORIGIN      
        1 agtcgtgatt acaggaagcg agtccacagg caagacgaca ctgtgcagaa aacttgctga
       61 gtattttcat acggaatggg ttcctgagta tggaagaatc tatctggaaa acaagggctc
      121 tgaaatggaa aaatcggatc tggaaatcat agggaaaggt caggctgaac tcgaagacag
      181 actctctctc agtgcagaaa aggtactgat ctgcgacact gaccttatca tcacagagat
      241 gtattcggaa tggtactatt ccttctgtcc ggaatctgta aggaaggaga ttatcagaag
      301 aaagtatgat ctgcatatcc ttctggaacc ggatgttccc tgggtgaagg acggactgcg
      361 ggatttccct gacagaagaa ttgagttcag ggatgaactt ctgcgaaaac ttgccgaata
      421 cgggtgcaga actgtcagag tcagcgggaa ttacgaggat agattcgaga aaacagttaa
      481 actgatatta gacatccttt cttcaaaaaa agaataaaaa aatctggaaa ttttatgcat
      541 tgaaatatca agtgtctatt taatgcttcg gagaatttta agaatcgtca gtctgttcat
      601 actttctgtc tttctgtgta tttctcctat gagcgacagg ctcgaacagc aggatgaaat
      661 cattcggcca gtccatccag tgatggaaaa cattgatttt actatgtttt ctgattattt
      721 tccagaatac agaagacttt ctcaggactc tttcctgaag cttgtgctcg aaaagaaatt
      781 ttccttcctg atgtctcttt ccacaaagat cccttttcag caattttcaa aagctttttt
      841 attctcaagt caaatactgc tctattctct catttccctt ccccctcctg tctgatctta
      901 tttttaaaga aagattatga tacgaaggaa caggaaatca ccatgcattt atatgtactt
      961 atatttttaa ccgcatctgc attcagtctg atttcggcac aggaaaaaaa tcctaaggat
     1021 tcaggcagtt cagccaaggt tgtgagaatg aaacttcagg acgcagaaga aaagtttctg
     1081 aagaacaatc ttcagctcct ggctcagaaa ttccagatcg acggaagccg tgccagaatt
     1141 ctgcaggcaa gactgtatgc c
//
LOCUS       DAOQGJ010001713         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103560_length_1160_cov_2.135747, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001713 DAOQGJ010000000
VERSION     DAOQGJ010001713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103560_length_1160_cov_2.135747"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1160
                     /locus_tag="PK683_20915"
     CDS             <1..>1160
                     /locus_tag="PK683_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10971.1"
                     /translation="DTRYPFLKVKKEKETILMGINPSKQQHEVLKLIKNQNTIQNAAK
                     GLLFSLNGSFQEQLQMDAKTLIGNLNQLVNNNLIAQVLVFEYDTYSKSLNVTPYFITH
                     PTEDKNYTLYMIFEDLIVYSYNSIRSWFEARDIMNSDSYLAEFKWQLNTKPGEEYKGI
                     SFVFNPTKRLYEVKNGVVIDQVMLDYIAKEVTRRILDSKIGIEVPGHGLLMLKTQEIQ
                     EFFESAEDFINTEMIPPLIEDVNHKFRIESLLFERKSYSVLEKFPRKTSKFSIALVKE
                     IRLIKSQSGRITYPGSLAIESMIRLENFIEDKYSELWRLECERVKKEFKKNITSQSTK
                     WTNLIAFVSHSDSLKFHPDVWRDLVNDPELFCMKWQTAKTTVHVFTGREAGF"
BASE COUNT          370 a          230 c          248 g          312 t
ORIGIN      
        1 agacacaaga tatccgtttc tgaaagtcaa aaaagaaaaa gaaacgattc tgatgggaat
       61 aaatccgtcg aaacagcagc atgaggtatt aaaactcatc aagaatcaga atacgattca
      121 gaatgccgca aagggattgc ttttttcgct gaacggttcg tttcaggaac agctgcagat
      181 ggatgccaag actttgatcg gcaacctgaa tcagctggta aacaacaacc tgatagcaca
      241 ggtactggta tttgaatacg atacctattc caagtcgctg aatgtcaccc cttactttat
      301 cacccatcct actgaagaca agaattatac tctgtatatg atttttgagg atctgattgt
      361 atattcctac aattcgatcc gttcctggtt tgaggccagg gatattatga attcagattc
      421 ctatcttgct gaattcaaat ggcagctgaa tacgaagccg ggtgaggaat acaagggaat
      481 atcgtttgta ttcaacccca ccaaaagact ttatgaagtg aaaaacggtg ttgtcattga
      541 ccaggttatg cttgactaca ttgccaagga agtgaccaga cggattcttg acagcaaaat
      601 cggaattgag gtgcctggac atggacttct gatgctcaaa acacaggaaa tccaggaatt
      661 ttttgagtct gcggaagatt tcatcaatac tgaaatgata cctcctctga ttgaagatgt
      721 aaatcacaag ttcagaatag aaagccttct ttttgaaaga aagtcttaca gcgttctgga
      781 aaagtttccc aggaaaactt ccaaattttc cattgcactt gtcaaggaaa tcagactcat
      841 caaatctcag tcgggaagaa tcacttaccc cggttctctt gcaattgagt cgatgatccg
      901 tctggaaaac ttcattgaag acaaatacag tgaactatgg cgtcttgagt gtgagagagt
      961 gaaaaaggaa ttcaagaaga acatcacttc tcagtcgaca aaatggacaa acctgattgc
     1021 atttgtttcc cattcagatt cactgaaatt tcatcctgat gtctggaggg accttgtcaa
     1081 tgaccctgag ctcttctgca tgaaatggca gactgcaaag acgacggtgc atgttttcac
     1141 gggaagagaa gcgggctttt
//
LOCUS       DAOQGJ010001714         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103623_length_1159_cov_3.788949, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001714 DAOQGJ010000000
VERSION     DAOQGJ010001714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103623_length_1159_cov_3.788949"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PK683_20920"
     CDS             <1..701
                     /locus_tag="PK683_20920"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNH10972.1"
                     /translation="LPYVGEELENKGYVTELVKEVFQSQGITADIQFLPFARILNMAK
                     EGRIDGYFPEYMNEENKEIYYYSDPYPGGDIGFLKLKKGAYESDSFKETKDFHSLEKF
                     RIGIVRGYVNTAEFDKADYLRKEEAASDLLNLKKLVFGRVDMIVIDRNVSEYLASRYL
                     GQYNADQSSLQFIEPGLDYKELYTCFSKKSEYGQYYTQKFNSGLSHLKNTGKLKEILD
                     RNNFLNGRYKNSRN"
     gene            complement(706..1059)
                     /locus_tag="PK683_20925"
     CDS             complement(706..1059)
                     /locus_tag="PK683_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10973.1"
                     /translation="MIPQNVLDEINKLDLEKKDKVLKYIQREQEMGGEPAPEEILEII
                     KYLKSKVVSRNRSADKERAILDFEILPRDYMRIYNCHTCRFKRGNKCSNQGAPLFEEE
                     IVNELLCVHYAFFGG"
BASE COUNT          362 a          214 c          242 g          341 t
ORIGIN      
        1 ggcttcctta cgtgggagaa gaacttgaaa acaagggtta tgtcacagag cttgtgaaag
       61 aggttttcca gtctcagggt attactgccg atatacagtt tcttccgttt gcaaggatac
      121 tgaatatggc aaaggagggc aggattgacg gatattttcc agaatatatg aatgaggaaa
      181 ataaagaaat ttactattac tccgatcctt atcctggcgg agatatcgga tttttgaaac
      241 tgaaaaaagg tgcttacgag tcagattcct tcaaggaaac gaaagatttt cattctctcg
      301 aaaaattcag gatcgggatt gtccgaggat atgtaaatac ggcagagttc gataaggcgg
      361 attatctgag aaaggaagag gccgcaagcg atcttctcaa tttaaaaaaa ctggtattcg
      421 gtagagtcga tatgattgtg atagacagaa atgtttcaga gtatcttgcc tcaagatacc
      481 tgggtcagta caatgcagat caaagctctc ttcagttcat tgaaccggga cttgactata
      541 aggaactcta tacatgtttt tccaaaaaat cggaatacgg acagtactat actcagaaat
      601 tcaattcagg actttcgcat ctaaagaata cagggaaact gaaggaaatt ctggacagga
      661 acaattttct gaacggaaga tacaaaaaca gcaggaactg agtgatcagc ccccaaagaa
      721 ggcatagtga acgcagagga gctcatttac aatttcttct tcaaacagag gggctccctg
      781 atttgagcat ttgtttcctc ttttgaatct gcatgtgtgg caattgtaaa ttcgcatata
      841 gtctctcggc agaatctcaa agtcgagaat ggctctttcc ttgtccgcag acctgtttct
      901 ggaaacgacc ttgcttttca gatatttgat gatttccaga atttcttcag gagcaggctc
      961 tccgcccatt tcctgttctc tctggatgta tttcagcacc ttgtcttttt tttcaagatc
     1021 cagcttgtta atctcatcga gaacattctg aggaatcatg cgaatagatt ccctttggcc
     1081 gtaaaatatt caagaaaatt ttttcaaaat attatattat tcatttaatt aaaaatggct
     1141 aatatttaag accaacaca
//
LOCUS       DAOQGJ010001715         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103628_length_1159_cov_3.551630, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001715 DAOQGJ010000000
VERSION     DAOQGJ010001715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103628_length_1159_cov_3.551630"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PK683_20930"
     CDS             complement(<1..439)
                     /locus_tag="PK683_20930"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNH10974.1"
                     /translation="MRAVFCILLFQNCLGYLAHLGKGQAAILYGRKKISDLVQSENTP
                     EKQKSKLKTIVHARLFALEKLSLNPKGGFEYFTSLDREEVGWHVTASYPLKFESYTWW
                     FPIAGRVPYKGFFDYAMAKELEDSLISEGLDTRLRVTAGYSTLG"
     gene            complement(439..921)
                     /locus_tag="PK683_20935"
     CDS             complement(439..921)
                     /locus_tag="PK683_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH10975.1"
                     /translation="MSRNTETIIKFYKAFQDKNPQSMIECYSDDIEFYDPAFQNLKGI
                     EAKAMWQMLIERASDLKLNFTNVQADENKGSADWVAEYPFSKTGRRIVNRIHAEFIFK
                     DGKIIRHTDSFNLWKWSGMALGPIGYLLGFTPFIQNQIRKEARTGLELYMKRKRMKES
                     "
BASE COUNT          288 a          265 c          230 g          376 t
ORIGIN      
        1 atcccagtgt ggagtagcct gcagttactc tgagtctggt atcaagacct tcacttataa
       61 gggagtcttc cagttccttg gccatcgcat agtcgaagaa tcctttatag ggaactctgc
      121 ctgcaatagg aaaccaccag gtataggatt caaatttcag cgggtacgat gctgtaacat
      181 gccatccgac ttcttctctg tccagagatg taaagtattc aaagccccct ttcggattca
      241 gtgaaagttt ttccagtgcg aacagtcttg catgaacaat cgtcttaagt ttagatttct
      301 gtttttccgg tgtattttca ctctgcacca gatctgatat tttttttcga ccgtaaagaa
      361 tggcggcctg cccctttccc agatgagcaa gatacccgag gcagttctgg aaaaggagaa
      421 tgcagaaaac tgcacgcatc agctttcttt cattctttta cgtttcatat agagttcaag
      481 tcctgttctg gcttcctttc tgatctggtt ctgaatgaac ggcgtgaatc cgagaagata
      541 accgatcggt cccaaagcca tccccgacca tttccaaaga ttgaaactgt cagtatgcct
      601 gatgattttt ccatccttga atataaactc ggcatgaatc ctgtttacga ttcttcttcc
      661 agtcttggaa aaagggtatt ctgccaccca gtctgcgctg cccttatttt catccgcctg
      721 aacgtttgta aaattcagct tgagatcaga ggctctttca atcagcatct gccacattgc
      781 tttggcttca atccctttca gattctggaa tgcaggatcg tagaattcta tgtcgtctga
      841 ataacactca atcattgact gcggattctt gtcctgaaat gccttgtaaa actttatgat
      901 tgtttctgta tttctgctca tcggaacctc tgtctgggtt ttgtcctgtc cggtacggct
      961 gtgcctcacc gaaatgactt ttgcataatc tgatttagga atttccatct ggcaagtatt
     1021 ttttcttcag tctgaagaaa atcggacgtt gatcgcttta aaacgacagt actttcgctc
     1081 cgtccataaa ataagttaaa taaatggcct taagttctcg aatttatata tatcaggtct
     1141 ttgaatttcc tgggaactg
//
LOCUS       DAOQGJ010001716         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103656_length_1159_cov_3.057065, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001716 DAOQGJ010000000
VERSION     DAOQGJ010001716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103656_length_1159_cov_3.057065"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PK683_20940"
     CDS             <1..822
                     /locus_tag="PK683_20940"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNH10976.1"
                     /translation="LDPYAGAAFAVAESARNVFVTGARPLGITNNLNFANPYIPENYY
                     VFSECIRGIGDACRHFGLPVTGGNVSFYNESPSGPVYPTPTIGMVGLISDIRNAVHPF
                     IQKAGLKVCLLGKFKPTLGGSEYLREIHSLTKGGIPSMNLEDEKNLQKAYLELAGKGI
                     LKSGKDLSLGGIVTAAFKTAADSRFGLVLDLKNISSLRLDEKYFGETAASVIVTCNDS
                     DFTEVQKTAEKNGIDCIEIGKTVSERILRIEELGAEFSLDSLKKDYESRLREYFV"
     gene            822..>1159
                     /locus_tag="PK683_20945"
     CDS             822..>1159
                     /locus_tag="PK683_20945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNH10977.1"
                     /translation="MDGLLILLSITAAGGLWLRFVYRYDKVEPEPIWTVLRIGVFGGL
                     TAAVLAVIGNGLFEWITGLDAGRRNSFLGSFFLAGFVGIWEELCKAFAAVLFLDKLEE
                     MDEPIDALIYS"
BASE COUNT          293 a          234 c          314 g          318 t
ORIGIN      
        1 ctggatccat atgcaggggc agcttttgca gtggcagaat cggcaagaaa cgtatttgtc
       61 acaggggcta gacctctcgg aatcacaaac aatctgaatt ttgccaatcc gtacatacct
      121 gagaactatt atgtattttc cgaatgcatc aggggaatag gggatgcctg caggcacttc
      181 ggtcttcctg tcaccggagg caacgtttct ttttacaatg aatctccctc tggacctgtg
      241 tacccgactc ctacgatcgg gatggtcggg ctgatttccg acatcagaaa tgcagttcat
      301 ccttttattc agaaggccgg actgaaggta tgtctgctcg gaaaatttaa acccactctc
      361 ggaggttccg agtatctgag agaaattcat tctctgacga agggtggaat tccttcaatg
      421 aatctggagg atgaaaaaaa tcttcagaaa gcgtatctgg aacttgcagg gaagggaatt
      481 ctgaaatccg gaaaggatct ttctctggga ggaatcgtca cggctgcatt caagacagca
      541 gcggattcca ggtttggtct tgttctggat ctgaagaata tttcctcatt aagactggat
      601 gaaaaatatt tcggagagac agcagcatct gtcattgtca cctgtaatga ttcggatttt
      661 accgaggttc agaaaactgc agagaaaaac ggaattgact gcattgaaat tggaaagacg
      721 gtttctgaaa gaattctccg aatcgaagaa ctgggtgcgg agttttctct ggacagtctg
      781 aagaaggatt atgaaagccg tctgagagag tattttgtct gatggacggg cttctgatac
      841 tcctttcgat cactgccgca ggcgggctat ggctcaggtt cgtatacaga tatgacaagg
      901 tagagccgga gccgatctgg actgtgctca ggataggagt tttcggaggc ctgactgcag
      961 ctgtgcttgc ggtcatcggg aacggacttt ttgaatggat caccggactt gatgcgggcc
     1021 gcaggaattc ttttctgggc agtttttttc tcgccggatt tgtaggaatc tgggaggagc
     1081 tctgcaaggc ttttgcggct gttctgtttc tggacaagct tgaagagatg gatgagccga
     1141 ttgatgctct gatctattc
//
LOCUS       DAOQGJ010001717         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103794_length_1158_cov_3.694470, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001717 DAOQGJ010000000
VERSION     DAOQGJ010001717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103794_length_1158_cov_3.694470"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..847
                     /locus_tag="PK683_20950"
     CDS             476..847
                     /locus_tag="PK683_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNH10978.1"
                     /translation="MKLSVGNLPQSLTDEDLKKLFASVGSVESASIKKDKKTGTSLGY
                     GTVEMADDHAKLAIEKLNGHEIEGKKIVVVDAASLQQDSKNKGSQKAGQGSGGSIHGS
                     KVSGGFSGGTVRRSGGGGRGK"
     gene            847..>1158
                     /locus_tag="PK683_20955"
     CDS             847..>1158
                     /locus_tag="PK683_20955"
                     /inference="COORDINATES: protein motif:HMM:NF027399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10979.1"
                     /translation="MAKLLNEVQSSGKKFFFQFGGQGSPYLKEVTKLYKDEPLLKEYF
                     ETVFRCFGKQESRFTKSDRRFEFGYDLKSWMENPDSAPSEDYQARASVSVVMIFVTQV
                     AH"
BASE COUNT          356 a          238 c          270 g          294 t
ORIGIN      
        1 agcaataaca gcagcttcca cttgtgcgag cctccaaacc gctgacaggc gtaaggtgta
       61 agaaactgtc cgctttactc cggtttactc aatatgaatg attcaaatac cgtcaggcgg
      121 aaacattaca atggaaaagt cgccatccaa tgaattcata acagcaaaca tcgccattct
      181 cgttccagcg gctttttgac agctgaaatt ctcttatccg ccacccgcat tcataaaagc
      241 ttgggaatca gtttcccttt cgaaccaaaa tcatataatg ggtccatgga aagtattttg
      301 cagtcgccgg aagtttgatt tgccgctgaa taacagaaat catccgaata aggaaattgt
      361 ctttcccaga taagtgcttt catttgtcgg ggcacatatg cgttgctgtg cccttgctgg
      421 aacaaaaaca gatttttcgc ttttcataaa acactgataa atttactttt acagtatgaa
      481 actatccgtt ggaaaccttc ctcaaagttt aacagacgaa gatctgaaga aattattcgc
      541 ttcagttggt tcagtagagt ccgcttcaat caaaaaagac aaaaaaaccg gaacctctct
      601 gggttacggc acagtggaaa tggctgatga tcatgccaaa ctggctatag aaaagctgaa
      661 cggccatgag attgaaggaa agaagatcgt tgtggtggat gcagcatcac tgcagcagga
      721 ttccaaaaac aaaggttctc agaaagcagg tcagggatct ggaggcagta ttcacggctc
      781 caaggtcagc ggcggcttct cagggggcac agtgagaaga tccggcggag gcggaagagg
      841 gaaataatgg caaaattact gaatgaggtt cagtcctcag gaaaaaaatt cttctttcag
      901 ttcgggggac agggttctcc ttatctgaag gaagtgacaa aactgtacaa ggatgagcct
      961 cttttaaagg aatactttga aacagtattc agatgttttg gaaagcagga gtccagattt
     1021 actaaatcag acagaagatt tgaattcgga tacgatctga aatcctggat ggaaaatccg
     1081 gattcggctc cttctgaaga ctatcaggcg agagccagcg tgtctgtagt gatgatcttt
     1141 gtcactcagg tggctcat
//
LOCUS       DAOQGJ010001718         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103881_length_1158_cov_2.312783, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001718 DAOQGJ010000000
VERSION     DAOQGJ010001718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103881_length_1158_cov_2.312783"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..919)
                     /locus_tag="PK683_20960"
     CDS             complement(227..919)
                     /locus_tag="PK683_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10980.1"
                     /translation="MKKADGAYFKRFGVIRSFRQDLKDIEKKDFRRVFHIAPKKYGFY
                     AFPDGLIEPYLIINTEPFHSSQKSVWIKDENGKKISFENPEEYLWRTAKNLSQERKKL
                     IRSKKIKLKTVSHIKDGGNTGEYFLTAFRHPRKFRHRGYVWSHLHTLDGFPKELENKG
                     VVIRSWIMLGWEDYSYALQKTVSALKERIWKSGSKDVKFDFHIFRFFLKREWRHWCSL
                     DDFEVFIEKWKP"
BASE COUNT          316 a          253 c          232 g          357 t
ORIGIN      
        1 atctttgtaa aacagaatgt gaatctgtgg gttaaccctt actgacaacc tgttgcgaag
       61 ccatcaggcg ggcaacaggt tggtctgcaa ggtttcgcct tacaggcgaa agggtggacc
      121 tgctgcgaag ccatcaggcg ggcagcaggt ctaccttcgg tcgccggttt gcggttgtct
      181 taaagcataa aaaaatgatg tttatgcgga gacgctgcta tcagcttcaa ggcttccatt
      241 tttcaatgaa aacttcgaaa tcatccagac tgcaccaatg gcgccattcc cgctttaaaa
      301 agaatctgaa aatatgaaaa tcaaatttca catctttaga tcccgatttc cagattctct
      361 ctttcagagc agagactgtt ttttgaagag catagctgta gtcttcccag ccgagcatga
      421 tccagctccg gatgacaaca cctttgtttt ccagttcctt gggaaaccca tccagagtgt
      481 gcaggtgact ccagacataa cctctgtgtc tgaatttgcg gggatgtctg aatgcagtta
      541 aaaaatactc tccagtattg ccgccgtctt tgatatgact gacagttttc agttttattt
      601 ttttactgcg gatcagtttt ttccgctcct gactcagatt ttttgcagtt ctccaaaggt
      661 attcttccgg attttcaaag gaaatcttct ttccgttttc atccttgatc caaacggatt
      721 tttgagagct gtgaaaaggt tctgtattga tgatgagata aggctctatc agaccgtcag
      781 gaaaggcata gaatccgtat ttcttagggg cgatatggaa gacccttctg aagtcctttt
      841 tctcgatgtc tttcagatcc tgtctgaaag aacgaatgac cccaaaacgc ttaaaataag
      901 caccgtctgc ttttttcatc agtattctcc tgttaagaat tactaatgag cagtggaacc
      961 tcctttatta taagctttca tatcaatctc cctgtatttt atgacggcag aatctgaaat
     1021 ttccttagca caaagagttt ttttcaatca actcgtaaaa tttttcttcc tctataaaag
     1081 agaggcttca aggatgaaac tgtcactggc tgataccgac aaaaatccta gtaaaagctt
     1141 ataatcagtc acagcata
//
LOCUS       DAOQGJ010001719         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103911_length_1158_cov_1.960109, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001719 DAOQGJ010000000
VERSION     DAOQGJ010001719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103911_length_1158_cov_1.960109"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PK683_20965"
     CDS             complement(<1..647)
                     /locus_tag="PK683_20965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH10981.1"
                     /translation="MISASLKSSLSLIPDFTDREKVFRILRRRIAEEGFFNVLKTDDF
                     SLFHEKLRDFSLQRFSPGLPVAVMAQVNVAGKILRTVSDSGSQEAERILNDIILGNIS
                     ASMGISEKGWKGRTSNLKTVIRFSENFGIIDGEKSFFTNGANSELLIILCFDSDRNCF
                     AAAVEKETEGLKTENFSLDFAAEATHCSAVFQNLRVPKERIFPLDYRKIGVQTVF"
     gene            complement(644..>1158)
                     /locus_tag="PK683_20970"
     CDS             complement(644..>1158)
                     /locus_tag="PK683_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="endoflagellar motor protein"
                     /protein_id="HNH10982.1"
                     /translation="TLVLDGNSGIIRDPDLVPKSQVYRLWEKLTSVIKKMVLTDKVLI
                     QLQNKDELTLTIPASSLGEGKIRLPEETEELFKKLSDTDKEVPLEITVRVQIPYLENS
                     DRTVFKNNWEYNAHRASIVAKYISAKFGIPEERISVQGLSQFKKSRETMTPEEEALDE
                     RLEIMVRKKE"
BASE COUNT          280 a          272 c          242 g          364 t
ORIGIN      
        1 aagaagacgg tttggacacc gatctttctg taatcgagag ggaatattct ttccttcggc
       61 actctgagat tctgaaacac tgcactgcag tgagtggcct ctgctgcgaa atccagagag
      121 aaattttcag tcttcagtcc ttctgtctct ttttccactg cagccgcaaa acagtttctg
      181 tcggaatcaa aacacaggat gatcaggagt tctgaattgg caccgtttgt aaaaaaggat
      241 ttttcgccgt caatgatccc aaagttttcg gagaaacgga tcactgtctt cagattggaa
      301 gttcttccct tccagccctt ttcggaaatt cccatggatg ctgagatatt tcccagtata
      361 atatcattaa gaattctttc cgcttcctga cttcctgaat cagaaacagt gcgcagaatt
      421 tttcctgcca cgttcacctg ggccatcact gcaacaggga gtccgggact gaatctctgc
      481 agagagaagt ctctcagctt ttcatggaac agagaaaaat catccgtctt cagtacattg
      541 aaaaaacctt cttccgcaat tcttcttctg agaattctga ataccttctc cctgtcagta
      601 aaatcaggga tgagacttaa ggaggatttg agacttgcac tgatcattct ttttttctta
      661 ccatgatctc gagtctctca tcgagtgctt cttcttcagg agtcattgtc tccctggatt
      721 ttttaaactg ggaaagtccc tgaactgata ttctttcctc aggaatcccg aatttcgcgc
      781 tgatgtattt tgccacgatg gaagccctgt gagcattgta ttcccagttg ttcttgaata
      841 cggttctgtc cgaattttcc agatagggga tctgcactct gactgtgatt tccaaaggca
      901 cttccttatc ggtatcagag agttttttga aaagctcttc tgtctcctca ggcagtctga
      961 tttttccttc gccgagggag gaggcaggta tagtcagtgt aagctcatcc ttgttctgaa
     1021 gctggatcag cactttgtct gtcagcacca tctttttgat gactgaagtc agtttttccc
     1081 aaagtcggta cacctgagac ttcggtacca gatcaggatc tctgatgatt cctgaatttc
     1141 cgtccagcac aagtgtct
//
LOCUS       DAOQGJ010001720         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_103931_length_1158_cov_1.697189, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001720 DAOQGJ010000000
VERSION     DAOQGJ010001720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103931_length_1158_cov_1.697189"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PK683_20975"
     CDS             <1..226
                     /locus_tag="PK683_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10983.1"
                     /translation="SFCETDYFSLTTKTVIHGHISVKTVSNTGNNWSTDTGCGFGGRL
                     SAIILPEKKIIQSSEKSKNWYLELISLQSE"
     gene            403..>1158
                     /locus_tag="PK683_20980"
     CDS             403..>1158
                     /locus_tag="PK683_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10984.1"
                     /translation="MNKLIILATFLLGMNHIYATSVTAKAQETRVDLLNYLRQLEPMV
                     KNYPGKDKDNKDVNPLDIVTKDDGSSKGDRIVKYDAIKRLYLEGVMYYYEGRFINSYR
                     RFLEAQINTEQLMEELSQYYIEGTDEILKSAMEMRSDADQNMKGKDGNPPQNDRDVVD
                     ISVESGRGAAIFPIHRTPRQAGEHVRDYNPKDYHYVLNKDAIEGSAAMGYKLLSQAKT
                     EKLGALKIEKNLEKHQKLTPDHRKQRIEMYFNVI"
BASE COUNT          423 a          208 c          243 g          284 t
ORIGIN      
        1 aagcttctgc gaaaccgatt atttctcact gacaacaaag acagtcatac acggccatat
       61 ctcagtaaaa acagtctcca atacaggaaa caactggtct acagatactg ggtgcggttt
      121 tggaggaaga ctttcggcca ttattctgcc cgaaaaaaaa atcattcaaa gcagtgaaaa
      181 atcaaaaaac tggtacttag aattaatatc attacagtca gaataggact caattccggc
      241 cgaaatattg atggaatgga agagaaagaa atctcaggtg aatctttttt aagaatgtac
      301 ttttttttgc gataaaatct gccgaaatag aaatttgagt tgtattcagg tgtgctgcct
      361 tcaacttaag aaaataagaa tcttgtgata gcaggaaaga caatgaacaa attgatcatt
      421 ttagcaacct tccttctggg aatgaaccat atatatgcca cttctgttac tgccaaggct
      481 caggaaacca gagtagatct gctgaattac ctgagacaac ttgagccgat ggtaaagaat
      541 tatccaggca aggataaaga caataaggat gtaaatcctt tggatatcgt tacaaaagac
      601 gatggatcat ccaaagggga cagaatcgtt aaatacgacg ccattaagag actttatctc
      661 gaaggtgtaa tgtattacta tgaaggccgt tttattaatt cttacagaag gtttctggaa
      721 gctcagatca acactgaaca gctgatggaa gaactttctc aatactatat tgaaggtaca
      781 gacgaaattc tgaaatctgc aatggaaatg agaagtgatg cagatcagaa tatgaaagga
      841 aaggacggaa atcctcctca aaacgacaga gatgtggtag atatttccgt tgaaagcgga
      901 aggggtgcag caattttccc tattcacaga actccaagac aggccgggga acatgtccgc
      961 gattacaatc ctaaggacta ccactatgtt ctgaacaagg atgcaattga aggcagtgct
     1021 gcaatgggtt ataaactcct gagccaggca aagacagaaa aactaggagc tctgaaaatt
     1081 gaaaagaatc tggaaaaaca ccagaaactg actcctgacc acagaaaaca gagaatagaa
     1141 atgtatttca atgtaatc
//
LOCUS       DAOQGJ010001721         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_104041_length_1157_cov_2.241379, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001721 DAOQGJ010000000
VERSION     DAOQGJ010001721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104041_length_1157_cov_2.241379"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PK683_20985"
     CDS             <1..344
                     /locus_tag="PK683_20985"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNH10985.1"
                     /translation="TSFGAHIWKKDKGVLNQHIFRVEFLSKCIKEYLYIALNRAVGEI
                     EENLHGGVGLVHITKGNLEEIQIPLPPIEVQERIVAKIEAERKSVDGCRDLMKTYEEK
                     IKRVIDRVWEG"
     gene            346..738
                     /locus_tag="PK683_20990"
     CDS             346..738
                     /locus_tag="PK683_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10986.1"
                     /translation="MKRKVFLCVSVLFLVSSFVLSLPNTDWKDNKSILNLVCLFLGTV
                     GSAISIFIPNNYIQLFTKDFWSKTDSGDWSITVPGKVHFLGSSPKVTVFQNENGLFSE
                     IIVDVSHTEKGEVVILSNIKFDGKAVIS"
     gene            783..>1157
                     /locus_tag="PK683_20995"
     CDS             783..>1157
                     /locus_tag="PK683_20995"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH10987.1"
                     /translation="MITKLHLKNFLSFRDTVVHFEDFNVILGANASGKSNLIKAILVL
                     KTFALEGVFHSEDSYSDFMETSFRKNSDPKENLYLECQMKNSFKYSNAKGELELTLND
                     YNYSLECSSEEGIIKEEYIAKLN"
BASE COUNT          385 a          176 c          242 g          354 t
ORIGIN      
        1 gtacttcttt tggagcacat atttggaaaa aagataaggg agttctaaat cagcatattt
       61 ttagagtaga atttttatcc aaatgcatta aagaatatct gtatatcgca ttgaatagag
      121 cagtcggtga aatagaagag aatcttcacg gtggagtagg tttagttcat atcaccaaag
      181 gcaatttgga agaaatccaa atccctcttc cgccgattga ggtgcaggag agaattgtgg
      241 ctaagattga agcagaaaga aagtctgtgg atggatgcag agatttaatg aaaacctatg
      301 aagaaaaaat taaacgggtc atagacaggg tatgggaagg gtgagatgaa gaggaaagtc
      361 tttttgtgcg tatcagttct ttttctggtt tcatcatttg ttctatccct tccaaataca
      421 gactggaagg ataacaaatc tatactaaat ttagtttgtc tttttctggg gacagtcggc
      481 tctgcgattt ccatcttcat tcccaataat tatattcaat tatttacaaa agatttctgg
      541 agcaaaacag acagtggtga ctggagcatt accgttcccg ggaaggtgca ttttttaggc
      601 agttccccaa aagtcactgt attccagaat gagaatggac tgttttccga gattattgtg
      661 gatgtctcac atacagaaaa gggagaagtt gtcattctct caaatataaa atttgacgga
      721 aaagctgtaa tcagctgata agtgaatgaa tgttctagag ttaaataatc cgaggagaaa
      781 ctatgatcac caaacttcat cttaaaaatt ttctcagctt cagggataca gttgtccact
      841 ttgaagattt taatgtaatt ttgggcgcga atgcaagcgg aaagtcaaat ttgatcaaag
      901 ccattttggt cttaaagact tttgctctgg aaggggtctt tcattcagaa gattcctact
      961 cagattttat ggaaacttct ttcagaaaga attctgatcc taaagaaaat ttatatcttg
     1021 aatgtcaaat gaagaattcc ttcaaataca gcaatgccaa aggtgaactt gagctgacac
     1081 tgaatgatta taattattca ctggaatgtt catctgaaga aggtatcatt aaagaagagt
     1141 atatcgctaa gttaaat
//
LOCUS       DAOQGJ010001722         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_104386_length_1155_cov_2.159091, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001722 DAOQGJ010000000
VERSION     DAOQGJ010001722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104386_length_1155_cov_2.159091"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1155
                     /locus_tag="PK683_21000"
     CDS             <1..>1155
                     /locus_tag="PK683_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10988.1"
                     /translation="GNITKVYYSSKNSFTDSGISLPSGSLWLAMDVNGDGRAEIFTHK
                     ADNDNLLRVTPVTDIFIKIQSHNESRKEARALRRGAELGSAAGAVGGALGGPVGSAVG
                     AYYGAQLGAGIVKYQASMNVNRISTSSLWGWLSFIAQLFYSQKSTIAYYPSPYASRQG
                     LLSTVSDSSPFFEKTVSVTYDYIRNFSSAVKIGTGSYPNLSFPSNETLVKTVSVSDGG
                     GRTDTDTYDYEDTRYIAGDRDSFAQLGFRKITVTHQPENTKEETLIYQTDKLLAGKTE
                     SVRAWNSAGSLSSESFHSYTAVSPFGRNLVLPQTQTAKKYRLGNLLLTSSETVSYDLY
                     GNPVSRSVSAGSQNSEEQNEYLNSADFDSYYLGRVSKARKLNQGTQVDSVS"
BASE COUNT          326 a          248 c          282 g          299 t
ORIGIN      
        1 cggaaacatc acaaaagtct attacagctc aaagaattcc tttactgatt ctggcatcag
       61 tcttccgtcc ggttctctct ggcttgcaat ggatgtgaac ggtgacggac gtgcggagat
      121 tttcactcat aaggcagaca atgacaatct tctgagagtg actcctgtca cagacatctt
      181 cattaaaatc cagtcgcaca acgagtccag aaaagaggca agggcactga gaagaggagc
      241 tgaacttgga tctgctgcag gtgcagtcgg cggagcttta ggcggccctg tcggaagtgc
      301 ggtcggcgcc tattacggtg cacagcttgg ggcgggaatt gtgaaatatc aggccagtat
      361 gaatgtaaac aggatttcca catcctcact gtggggatgg ctttccttca ttgcacagct
      421 tttctacagt cagaaaagta caattgctta ttatccgtct ccttatgcat ccagacaggg
      481 acttctgagc accgtcagtg acagcagtcc gttttttgaa aaaacagtaa gtgtaaccta
      541 tgattatatc aggaactttt ccagtgcagt gaagatcggc acaggcagtt atccgaatct
      601 ttcctttcct tcaaatgaga ctctggtgaa gactgtttct gtttcagacg gaggcggaag
      661 gacggataca gacacttacg actatgagga tactaggtac attgcaggag acagggattc
      721 ctttgcacag ctgggtttcc ggaagataac agtcactcat cagccggaaa atacaaaaga
      781 agagactttg atttaccaga cagataaact tctggcagga aagacggaat cggtcagggc
      841 ctggaattct gcaggcagcc tttcctctga atcctttcat tcttatacgg cagtgtctcc
      901 ttttggaagg aaccttgtcc ttccgcagac tcagactgca aaaaaataca gactgggaaa
      961 tcttcttctg acttcttctg aaacagtcag ctatgattta tatggaaatc cagtatccag
     1021 atccgtcagt gcaggaagtc agaattcaga agagcagaat gaatacttga acagtgcgga
     1081 ttttgacagt tattatttgg gaagggtttc gaaggcaaga aaactgaatc aagggacaca
     1141 ggtcgattct gtttc
//
LOCUS       DAOQGJ010001723         1154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_104484_length_1154_cov_3.479527, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001723 DAOQGJ010000000
VERSION     DAOQGJ010001723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104484_length_1154_cov_3.479527"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PK683_21005"
     CDS             <1..869
                     /locus_tag="PK683_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNH10989.1"
                     /translation="KNMIPKFKEFPYVIRLVSEILESNGSSSMASVCSGSLALMSGGV
                     PMKGSVAGVAMGLITSEDKKNFAVLTDIAGIEDHFGDMDFKLAGTRTGITAFQMDLKI
                     SGVGFDLLEKVFLQAERGRNHILNEMEKAISSSAADLSITAPRITMKSIPKDRIGELI
                     GPGGKNIRSIIEKSGADISIDDEGNVTVSSPNFESSQKALDLIEGMFAEIEVGKIYQG
                     KVKRITDFGAFVEILPGKEGLCHVSKLDFKRVNSVKDVVKEGEIIPVKVIGVDRSGKI
                     DLSRKDAISAAN"
BASE COUNT          389 a          225 c          246 g          294 t
ORIGIN      
        1 tgaagaacat gattcctaaa ttcaaagaat ttccttatgt aatccgtctg gtttctgaaa
       61 ttcttgaatc gaacggttcc agttcaatgg cttccgtatg ctccggctcg cttgcactga
      121 tgtcaggcgg tgttccgatg aaaggatcag ttgcaggtgt tgcaatgggt ctgatcactt
      181 cagaggataa aaagaatttt gcagttctga cagatattgc tgggattgaa gaccatttcg
      241 gggatatgga ctttaaactt gccggaacca gaacaggaat tacagcgttt caaatggatc
      301 tgaaaatttc cggagtcggt tttgatcttc tcgagaaagt attccttcag gcagaaagag
      361 gaagaaatca catactgaac gaaatggaaa aagcaatttc cagttcagca gcagatctct
      421 ccataactgc tcctagaatt acgatgaagt ccattcctaa ggacagaatc ggtgaactga
      481 tcgggcctgg cggaaaaaac atacgttcca tcattgaaaa atcaggtgct gacatcagca
      541 tagacgatga aggaaatgta acagtttcca gtccgaattt tgaatcttct cagaaagcac
      601 tcgacctcat tgaaggaatg tttgctgaaa tagaagtggg aaaaatttac cagggaaaag
      661 taaaacgtat cactgatttc ggagctttcg tagaaattct tccaggaaaa gaaggactct
      721 gccatgtttc gaaacttgat ttcaaacggg tcaattcagt aaaggatgtg gtcaaggaag
      781 gagagattat tccagtgaaa gtcatcggtg tagacagatc agggaagatt gatttatcca
      841 gaaaagacgc aatttcagca gcaaactgac agacatcggg actgtgcaga aactctcagt
      901 cccgttttta ctgtccttca gaatatctaa ttcaattcag acacagtcca gaaagaaccg
      961 aaaccgcctc caggaaaact ctgcacagcc cgtaaaaaat cctgataaat aaatatgtta
     1021 tacagattta ataaatacat aaaaattcag tcggcacagt tttcactcag ccggcagccc
     1081 gaggaaaaaa atgaaaattc tcaaaaaaaa actctcaaat gatctgattc tgctccatga
     1141 aaaaattccc aagg
//
LOCUS       DAOQGJ010001724         1154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_104529_length_1154_cov_2.551410, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001724 DAOQGJ010000000
VERSION     DAOQGJ010001724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104529_length_1154_cov_2.551410"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PK683_21010"
     CDS             complement(<1..853)
                     /locus_tag="PK683_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNH10990.1"
                     /translation="MTKRKSEEKPVVLIMAGGKGERFWPRSRESLPKQLQKVYSNKTL
                     LEETISRALLLTDISRIFIGTNPNLKKAILKSLPKFPKNNFILEPEPKNTAPIVALAS
                     LFFQKKFGNPVQIVLSADAYIQPPEKFKSTMQSAVEAANSGSLVLLGVEPNRPETGYG
                     YIAAGKKNGTAYEVKAFYEKPDLKTAMKYIRKKNFYWNPGIFIWKTESILSEFQRHAP
                     YILHPLEESSSASELQSIFKKLPSEAIDVAIMEKSSSIVMVDAEFQWDDVGSWISLER
                     VLKGDENG"
     gene            complement(859..1098)
                     /gene="hfq"
                     /locus_tag="PK683_21015"
     CDS             complement(859..1098)
                     /gene="hfq"
                     /locus_tag="PK683_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNH10991.1"
                     /translation="MTVKNNIQDFILNTARKEKIELTIYLLNGVPLKGKVTSFDNFTI
                     IIENESKQNLIYKHAVSTIIPAKVIRFQQEEKEPG"
BASE COUNT          306 a          249 c          218 g          381 t
ORIGIN      
        1 ttccattctc atcacctttc agcactcgtt ccaatgaaat ccaggaaccg acatcatccc
       61 attgaaattc ggcatctacc ataacgatgg aagagctttt ttccataatg gccacatcaa
      121 ttgcttcgga aggaagtttc ttaaaaatcg actgaagttc cgatgcagat gaactttctt
      181 ccaaaggatg aaggatgtat ggagcatgtc tctgaaactc agaaagaatt gattcagttt
      241 tccagatgaa aattccggga ttccagtaaa aattcttctt gcggatatac ttcattgccg
      301 tcttaagatc aggcttttca taaaatgcct tcacttcata ggctgtgccg ttcttttttc
      361 ctgcagcaat ataaccgtat ccggtttcag gcctattcgg ctccacacct agcagaacca
      421 gagagccgga attcgcagcc tccacggcag actgcattgt ggatttgaat ttttcaggcg
      481 gctgaatata ggcatccgct gaaagaacaa tctgcaccgg attcccaaac tttttctgaa
      541 aaaaaagact tgccaatgcc actattggag cagtgttttt cggttctggt tccaagataa
      601 aattattctt tggaaattta ggaagcgatt ttaatatcgc ttttttcaga ttcgggtttg
      661 tgccgataaa aatcctggaa atgtccgtca gaagcaatgc cctagaaatg gtctcttcca
      721 gcaatgtctt attagaatag actttctgaa gctgtttcgg aagagattct ctggatctag
      781 gccagaacct ctcccccttg ccgccggcca taatcagaac aacaggtttt tcctctgatt
      841 ttcttttagt cattcatttc aaccgggttc tttttcttcc tgctggaaac gaattacttt
      901 ggcgggtata attgtagaga ctgcatgttt gtagattagg ttctgtttac tctcattctc
      961 aatgataatt gtgaaattat cgaagcttgt taccttgccc tttaatggaa ctccattcag
     1021 aagataaata gtaagttcta tcttttcttt ccgggctgta ttcagaataa aatcctgtat
     1081 attgttcttc actgtcatac tcttaaccgt ctctttttgt gttttgtagt aaatttaggg
     1141 catcttgcca attc
//
LOCUS       DAOQGJ010001725         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_104643_length_1153_cov_3.226776, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001725 DAOQGJ010000000
VERSION     DAOQGJ010001725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104643_length_1153_cov_3.226776"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(569..>1153)
                     /locus_tag="PK683_21020"
     CDS             complement(569..>1153)
                     /locus_tag="PK683_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH10992.1"
                     /translation="RELGFNGENYWIYSNYDHDRNFAERNSWPEKPIAGEYLSFPSLK
                     NPSAKSHTAEIIAFTDYENFEKWKDSPWKKRGEVYEAFKTKIKESLLDYINGKFPGFK
                     DIVEFSELSTPVTNEHFTGHPMGMIYGLPCVPDRFNSAKCPWFSLQTPVENLYLTGAD
                     ASSPGVSGAMMGGLNVAIKLMDGFDFVKMLLRGK"
BASE COUNT          322 a          268 c          220 g          343 t
ORIGIN      
        1 tacagacaga ttttttactc tggattttac gaattcaaat atttccggga ctgcttcttc
       61 cggtgctgtc tacttttcat ctatagtact taattcaaat tgtaatactg atcgagcagg
      121 aagtacaact tcctatttca ctggatattt gtctacttcc agtcttccca gtccgtatac
      181 agcaaattct cttgcatcta cagatacaaa aaaaatcctt tatcgaacca tacaaacggt
      241 ttcaagcaca tatacagttc agataacagc aacctccagt gcaacaccga ctgggataac
      301 tgtgaccaga gccagtttcg ggaactgaga attcttatag aaataacaca gagggcttgc
      361 tgatcagctt tgaatgaaag cttcagtaag ctcacaaact cggaagatgc agatatgcgg
      421 ctgagtcata gttcgatact aaatttattg catttgcaat aaattttact cactatgacc
      481 ctctgcacgt tcagcagact cgccgggccg cgatcttttt taattaaaag cagcgggctg
      541 tttgcccgcc ttttatccgc agtaacgact actttcctct cagaagcatt ttcacaaaat
      601 cgaatccgtc catcagttta atggccacat tcagtccccc catcatcgca ccggaaactc
      661 cgggtgagga tgcatctgct cccgtcagat aaagattttc tacaggcgtc tgaagactga
      721 accagggaca tttggcagaa ttgaaacggt caggaacaca gggcagtcca tagatcattc
      781 ccataggatg tcctgtaaaa tgttcatttg tgactggggt ggaaagttca gaaaactcca
      841 caatgtcctt gaagccggga aattttccat tgatgtaatc cagaagactt tccttgatct
      901 ttgtcttaaa tgcctcatac acctcccctc tttttttcca gggagagtcc ttccattttt
      961 cgaaattttc gtaatcagtg aaagcgataa tctctgcagt gtgagatttg gcactcgggt
     1021 ttttcagact ggggaaggac agatattcac ctgcgatcgg cttttccggc caggaattcc
     1081 tttcggcaaa atttctgtca tgatcataat tagaatatat ccaatagttt tcaccgttga
     1141 atccaagttc tct
//
LOCUS       DAOQGJ010001726         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_104795_length_1152_cov_4.585232, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001726 DAOQGJ010000000
VERSION     DAOQGJ010001726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104795_length_1152_cov_4.585232"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK683_21025"
     CDS             complement(<1..285)
                     /locus_tag="PK683_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10993.1"
                     /translation="MKITKNFAPSLAAVFLNENQRTVMKKSATENLLKVFRRKLKELF
                     PYICSERKVFSNVDFSSEDFAVTFIQSDTELEFLRKIAPSLPPSVLNDPDI"
     gene            complement(282..>1152)
                     /locus_tag="PK683_21030"
     CDS             complement(282..>1152)
                     /locus_tag="PK683_21030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNH10994.1"
                     /translation="KRLFKQRSPYKGSHKTQVLDFLIERAIVDITADEESITVNEKRI
                     ESEIEKMMEHSGITDRVQFEKHLSEKSGMPFEMWVSDLPYQIKKSQLMQMRVTVRPPT
                     EDDAKKWYERNKDKLGYEVKYREIVMIPRNTGLDEERRVSNEMSQIQKEIRKDKSAFN
                     LIASGPRNQSPRGGYSDWISTSELVMKNRTLVGNLAMLPEGGISELFRDDRGRYCIIR
                     MDGKRRTPMEAIRKIVQDIIAREKMDESFMDWVRERRKEVPMFIYDKEYAAENKIETP
                     DESFNFNKIQQEK"
BASE COUNT          279 a          268 c          206 g          399 t
ORIGIN      
        1 aatatcagga tcattcagaa ccgagggcgg aagtgagggt gcaattttcc tgaggaattc
       61 aagttcagta tccgactgaa taaaggttac ggcaaagtcc tccgatgaaa aatcaacatt
      121 ggaaaagact ttcctttctg aacagatgta aggaaacagt tctttcagtt ttcttctgaa
      181 aactttcaga aggttttctg tggcgctttt cttcattact gtcctctgat tttcattcag
      241 aaagacagcc gcaagcgaag gtgcaaaatt ctttgtgatt ttcatttttc ctgctgtatc
      301 ttgttgaagt tgaaagattc atccggtgtt tctatcttat tctcggcggc atattccttg
      361 tcgtaaatga acataggcac ttcctttctt cgctccctga cccagtccat aaaagactca
      421 tccatttttt ctctcgcaat aatatcctga acgatcttcc gaattgcttc cattggagtc
      481 cttctctttc cgtccattct tatgatgcag tatcttcccc tgtcatccct gaagagctca
      541 gaaattcctc cttccggaag cattgcaaga ttccctacca aagttctgtt tttcattaca
      601 agctctgaag tggatatcca gtcagagtat ccccctcgcg gagactgatt tcttggaccg
      661 gaagcaatca gattgaaagc agacttatct ttcctgattt ctttctgtat ttgggacatt
      721 tcattggata cccttctttc ttcatccaga cctgtatttc tgggaatcat tacaatttct
      781 ctgtacttga cttcataacc gagtttgtcc ttgttccgtt cataccattt ttttgcatca
      841 tcttccgtcg gaggacggac tgtgactctc atctgcatca gctgagattt tttaatctgg
      901 tacggaagat cagaaaccca catttcaaaa ggcattcctg atttttcact gagatgtttt
      961 tcaaactgta ccctgtctgt aattccggaa tgctccatca tcttttcaat ttcagattcg
     1021 attctttttt catttactgt gatggactcc tcatctgcag taatgtccac gattgcccgt
     1081 tcgatcagaa aatccagcac ctgtgtcttg tgagaaccct tataaggact tctctgcttg
     1141 aaaagtcttt tg
//
LOCUS       DAOQGJ010001727         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_104810_length_1152_cov_3.402005, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001727 DAOQGJ010000000
VERSION     DAOQGJ010001727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104810_length_1152_cov_3.402005"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..161)
                     /locus_tag="PK683_21035"
     CDS             complement(15..161)
                     /locus_tag="PK683_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10995.1"
                     /translation="MKVIGTKKERTPEECINAQEKLIAELNQLRPVKKRKGILMKFRT
                     WRNR"
     gene            386..559
                     /locus_tag="PK683_21040"
     CDS             386..559
                     /locus_tag="PK683_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10996.1"
                     /translation="MYFDETIKKIESNLERLQELDRMLDALKTVMEELMESADLHSAE
                     IETVDLSKREDQI"
     gene            659..>1152
                     /locus_tag="PK683_21045"
     CDS             659..>1152
                     /locus_tag="PK683_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10997.1"
                     /translation="MTGRTDARLELELQGEKAANYLRFFLIFIFCAGTLAGFLVQNHV
                     STILGNYIAGIVIYSFTAALSIYFVKSGNFSYSVKYITMSLELAGFAFVLFGFLRLTD
                     PEKLTIAVNDIVLYAIYFILIAESALRFSPRFTLFTGISCTVMFTVLGFMIQKAGGDK
                     AVFGV"
BASE COUNT          293 a          247 c          242 g          370 t
ORIGIN      
        1 atctttccag ggacttatct gttccgccag gttctgaatt tcatcagaat gcccttgcgc
       61 ttttttacgg gtctgagctg attcagttcg gcaatcagtt tttcctgggc atttatgcac
      121 tcctcaggtg ttctttcttt ttttgttccg attactttca tttttctgca ttctgttttt
      181 cagactccct gtttaattta catccgattc ggcggaagtc aagcattctc tataggctga
      241 ctttaatcag atcaagatca gagtatcacc tgaatcctgt atgtcaaaac tttgcgtcca
      301 tacaatcagc agtttttaca aaactgtaag ataaaaaagc agattcgtta ttgatccgta
      361 accgacagtc agatacaatg caggtatgta cttcgatgaa acaataaaaa agattgagtc
      421 caatctggaa agactgcagg aacttgacag gatgcttgat gcacttaaga cagtaatgga
      481 agaactgatg gaatcagcgg atctgcactc tgctgaaatt gaaaccgtgg atctgtccaa
      541 acgggaggat cagatctaaa gtttattttc ctgccctcat tcacgaaaag ttttgattgt
      601 cgattatcct tgctgcttac aatgttgtat tcgggaatac attcagggtg agaattcaat
      661 gacaggaagg acagatgcaa ggctggaact ggagcttcag ggcgaaaaag cagcaaatta
      721 tctccgtttc ttcctgattt tcattttctg tgcggggacc cttgccggtt tcctcgtgca
      781 gaatcatgtt tcaacaatcc tgggcaatta cattgcaggg atagtgattt actcattcac
      841 cgccgccctg tccatatatt tcgtcaagtc agggaatttc agctattccg taaagtatat
      901 tacaatgtct ctggaactgg ccgggttcgc ctttgtgctc ttcggatttc tccgtctcac
      961 agatccggaa aaactcacca tcgccgtgaa tgatattgta ttgtatgcga tatattttat
     1021 actgattgca gaatctgcac tccgtttttc tcccaggttc actctattca caggaatctc
     1081 ctgcactgtg atgtttactg ttctgggttt tatgatccag aaagcaggcg gcgacaaggc
     1141 tgtattcggt gt
//
LOCUS       DAOQGJ010001728         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_104820_length_1152_cov_3.163172, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001728 DAOQGJ010000000
VERSION     DAOQGJ010001728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104820_length_1152_cov_3.163172"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1152)
                     /locus_tag="PK683_21050"
     CDS             complement(<1..>1152)
                     /locus_tag="PK683_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH10998.1"
                     /translation="EGTVKSVLKELVSKNQAVYREAGKTYQIYYGSSIHLQARIEEYV
                     PSVLNSDTQKVLDRHFPLRPIYADAYNAEYLTSRFYSRKLILESTLKDFIQFSADSEG
                     KTSVRKNEEELQNILQNYFETLLEKERKSGASGLFIYYLGNPKSVYAEAVQHLLKNSD
                     TGRNLAGVFSRYSFTEEESLKKFEAAERLSEDPEVLRIDSRISEDVKVFLSDIHERVE
                     HSVQKLFRRDSAEITVHHDFISQSQDLESVVSFILKKRYPSSPKINSEFVNREHLTPV
                     IRNTRKKILRAMIAGDRSVNFRTNGYGPDVAIFRSLFSAKKCLDTDKEAFRFHFEHPK
                     DYKGAPDEGLKKVILEIQKFLEKDSEKHSLDTLYSRLTAEPYGMYTEIIP"
BASE COUNT          292 a          245 c          252 g          363 t
ORIGIN      
        1 ggaatgatct cagtgtacat cccgtacggc tctgcagtga gtctgctata cagtgtatcc
       61 agactatgtt tttcagaatc tttttccagg aatttctgta tttcgaggat gacctttttc
      121 agtccttcgt caggagcacc tttgtaatcc ttaggatgtt caaaatgaaa acggaaagct
      181 tccttgtcag tgtccagaca ttttttcgca ctgaaaagac ttctgaaaat ggcaacatcc
      241 ggtccgtaac cgtttgttct gaaatttaca gatctgtcgc cagcgatcat tgcacgcagt
      301 atcttttttc ttgtatttcg gatcacagga gtgaggtgtt ctctgttcac aaattcagaa
      361 ttgatcttag gcgaggaagg ataccgtttt ttcaggatga aagagacaac ggattccaga
      421 tcctgcgact gggatataaa atcatgatgg actgtaattt cagcggaatc cctgcggaaa
      481 agtttctgaa cagaatgttc cactctttca tggatgtcgg agagaaacac tttcacatcc
      541 tcggaaatcc tagaatcgat tctcagtact tcaggatctt cggacagcct ttcagccgct
      601 tcgaattttt tcagactctc ttcttcggtg aaggaatatc tgctgaatac gccagcgaga
      661 tttctgcctg tatctgaatt tttcagaaga tgctgcactg cctctgcata gactgattta
      721 ggatttccca gataataaat gaacagaccg ctcgcgcctg atttgcgttc tttttccaga
      781 agggtctcaa aataattctg aaggatattc tgcagctctt cctcattttt tctgactgag
      841 gtttttcctt ccgaatctgc agagaactga ataaaatctt tcagtgtgga ttcgagaatg
      901 agttttcggg agtaaaatct ggatgtgaga tattctgcat tgtatgcatc cgcgtagatg
      961 ggacggagag gaaaatgacg gtccagaact ttctgtgtat ccgagttcag tacagaggga
     1021 acatattctt caatccttgc ctgaagatgg atggagcttc cgtaatagat ctggtatgtc
     1081 tttccggcct ccctgtacac tgcctgattt ttggagacca gttccttcag aacagacttg
     1141 actgttccct ct
//
LOCUS       DAOQGJ010001729         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_104974_length_1151_cov_3.552920, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001729 DAOQGJ010000000
VERSION     DAOQGJ010001729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104974_length_1151_cov_3.552920"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PK683_21055"
     CDS             <1..1054
                     /locus_tag="PK683_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH10999.1"
                     /translation="SNYPAVTVSLLNELSRKYHFKPDKEILAAVPKKNESSKKMSEAF
                     SELPSRLKSFQKFRENYFREIYALIEKGRLKSSSQRLINTFIDPELLIRDQYASLEKR
                     IIGSSLPGIFDNENYIPSFSDVISGKAYRQIRTPDDSVHDLVIGLDFSSASAVNDKEI
                     YKSVENFCRSFTGLYRRTNFSFYAFSESCSEIHIPFTGREILKERRVFTPFLKRILHL
                     KRKDVPLHAVIITDGLPADLDETIMTGYKLKKLRIDFTQIFLCGDSIPDAKKIKSVSE
                     VTGGSLYGITDPALLGLYLSEILDRYYGLRTLSAKTLSDVSFDDFTGKSSAKIISSHP
                     EETKKKTVKSFTFKKL"
BASE COUNT          384 a          241 c          221 g          305 t
ORIGIN      
        1 atcgaactac cccgcagtca cagtctctct tttgaatgag ctttccagaa aataccattt
       61 caaaccggat aaagaaatcc ttgccgcagt tcccaaaaaa aatgaaagct caaagaaaat
      121 gtctgaagcc ttctcagagc tcccttccag actgaaatca tttcaaaaat tcagagaaaa
      181 ctattttcgg gaaatttacg cactgattga aaaaggcaga ctgaaatcct catcgcaaag
      241 actgataaat acattcattg atcctgaact gctcatccgt gatcagtacg cttcactgga
      301 aaaaagaatc atcggctcct cacttccggg aatatttgat aatgaaaatt atattccaag
      361 tttcagtgat gttatttcag gaaaggccta cagacagatt agaactcctg atgactcagt
      421 ccacgatctg gtcataggcc ttgacttttc atctgcatct gcagtaaatg ataaagaaat
      481 ttataaatct gtcgagaatt tctgccgcag ttttaccggt ttgtacagaa ggaccaattt
      541 cagcttttat gccttttccg aatcctgcag tgagattcac attccattta ctggacggga
      601 aatcctgaag gaaagaagag tatttacgcc attcttaaaa cgaatactcc atctcaaaag
      661 aaaggatgtc cctcttcatg cagttataat tacagatgga ctgccagctg atctggatga
      721 gacaataatg acgggatata aactcaagaa actcaggata gattttacgc agatattttt
      781 gtgcggtgat tccattcctg acgcaaaaaa aatcaagtct gtctctgagg ttaccggagg
      841 aagtctgtac ggaattacag acccggcact tctgggattg tatctctcgg aaattctgga
      901 cagatactat ggactgagaa cactttccgc aaagaccctg tcagatgtat cctttgatga
      961 ttttacagga aagagctcag caaaaattat ctcgagtcat cctgaagaaa caaaaaagaa
     1021 aacagtaaag tcatttactt tcaagaaact ttagtatcta gaaacggaag acagaggata
     1081 aatcccgtca aaagagctag gccttccatt gacagcagag accagggata gtgtcccaga
     1141 tgattcagga c
//
LOCUS       DAOQGJ010001730         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105141_length_1150_cov_5.032877, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001730 DAOQGJ010000000
VERSION     DAOQGJ010001730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105141_length_1150_cov_5.032877"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /gene="bioB"
                     /locus_tag="PK683_21060"
     CDS             <1..951
                     /gene="bioB"
                     /locus_tag="PK683_21060"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNH11000.1"
                     /translation="LVRTAYEPREKYFGKSVRIHILDNIKNGHCPEDCGYCAQRKNAS
                     SGIEQYSMKSLDELYADAIAAKKNGAYRFCMVTAGTGPNDSTVEKLSGLIEKINSELG
                     LKVCLSAGLLDEAKAERLRKAGLDRYNHNVNTSEAHYGEICTTHTFAQRMKTLEFLDS
                     AGIGLCSGVIVGMGESFADIADMAFTVKKLGVISIPVNFFIPVEGHAIRNPSVMTPEL
                     CIRILSVFRLVNPDSEVRIAAGREGHLRSLQAMALYPANSLFASGYLNVKGSDMEETV
                     RMIRDLGFEPETDGEPLPEIKEDALYSSENIKSLYKFPKK"
BASE COUNT          349 a          223 c          280 g          298 t
ORIGIN      
        1 cttgtcagaa cagcttatga gccgagagaa aaatatttcg ggaaatctgt cagaattcat
       61 atactcgaca atataaagaa cggtcactgt ccggaagact gcggatactg cgctcagagg
      121 aaaaatgcat cttccggaat cgaacagtat tccatgaaat cattggatga attgtatgcg
      181 gatgcaattg ctgccaaaaa gaacggtgcc taccgtttct gtatggtgac tgccggaaca
      241 ggaccgaatg acagcactgt agaaaagctt tccggtctga ttgaaaaaat caattcagag
      301 cttggactga aagtatgtct ttcggcagga ctccttgacg aggcaaaagc agagaggctc
      361 aggaaagccg gcctggacag atacaatcat aatgtaaata catctgaagc gcattacggt
      421 gaaatctgca caactcatac ttttgctcag aggatgaaaa cacttgaatt tctggacagt
      481 gcaggaatag gactctgcag cggtgtcatt gtcggtatgg gagaatcatt tgccgatatc
      541 gcggatatgg catttacagt caaaaaactg ggagtgattt ccatccctgt aaactttttt
      601 attcccgtag aaggtcatgc aattcgcaat ccttcagtga tgactccaga actgtgcatc
      661 agaatactgt cagtattcag acttgtcaac ccggactctg aagtaagaat cgccgccgga
      721 agggaagggc atctgagaag tctgcaggca atggcgcttt atcctgcaaa ttcacttttt
      781 gcttcaggtt atctgaatgt aaagggaagc gatatggaag agacagtcag gatgatcaga
      841 gatttaggct ttgagccgga gacggacggt gaaccgcttc ctgaaattaa agaagatgca
      901 ctgtattctt cggaaaatat taagtcactt tataaatttc ctaagaaata aaggttttct
      961 ggatgggctg gatacaaatg aggcagactg tatcatttgt aaaaacagga tacagtttgt
     1021 gtaagagcat aaaaaataag gctcaggcgg agcttgcact gagcctaccg attgtggtgc
     1081 ggagttcaaa gagcactgtt tttgcttcgc cgcttcgccg cttcgctatt gatcaaaaat
     1141 cattttgctt
//
LOCUS       DAOQGJ010001731         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105170_length_1150_cov_3.315068, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001731 DAOQGJ010000000
VERSION     DAOQGJ010001731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105170_length_1150_cov_3.315068"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..1007
                     /locus_tag="PK683_21065"
     CDS             429..1007
                     /locus_tag="PK683_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11001.1"
                     /translation="MKFSSFWAVLFFFFHCISSNTERFSEVPDERKNGFRDIVRFKKG
                     NSVSGVKAVETRGTVVVTSKDGESTVYNSREIFRIEKNAFDPESDSNTASSSEWSAFQ
                     GEMSWQEAKDACEAKEMRLPARAELLSAQRAGAWEHWKKDGMYSWYWSSEEYSAERAY
                     SVGIHGESVYSLKSDRYHYVRCIRSQKQGFGR"
BASE COUNT          347 a          195 c          261 g          347 t
ORIGIN      
        1 agatgctccg atgaaactct taatcaaata tcgtaatttg ctttggcaat atataatcat
       61 tgattcaata ttttttaaaa aatgcatttt cactcattaa ttattttcaa tgcgtaccca
      121 atcttgggta tttttcagat acagagtctt tcaggataat ttcagtaaaa attcttattt
      181 taaagaacct gtcaatattg acaggtattg aatgactgca atttcaggat gaagaaaact
      241 gaattgtctt atgcaggctc caggccgata tactgtctgt atgattcgag gatcatcttt
      301 attgaaaact atgcttcttt cagatcttaa gggacagggt cgatttttaa gatagtagaa
      361 aactcttatt ggatgttcat tgtatctgct tgttaataaa aaatcttata ctctatggga
      421 ttacagatat gaaatttagt tctttttggg cggttctgtt tttcttcttt cactgtatct
      481 cttcgaatac agagcgtttt tccgaagttc ctgatgaaag aaaaaacggt tttcgggata
      541 tcgtcaggtt caagaaagga aattctgttt caggtgtgaa ggctgtcgag accaggggaa
      601 ctgtcgtcgt gacctcaaaa gacggtgaga gcactgttta caattccaga gaaatattca
      661 gaatcgaaaa aaatgccttc gatcctgaat ctgattcaaa tacggcttca tcaagcgaat
      721 ggagtgcctt tcagggagaa atgagctggc aggaagcgaa ggatgcctgt gaagcaaagg
      781 aaatgcgcct gcctgcgaga gcagaacttc tttcagcaca gagagctggt gcctgggaac
      841 actggaaaaa ggacgggatg tattcctggt actggagttc agaggaatac tctgcagaga
      901 gggcctactc agtcggaatt catggggaat ctgtctacag tctcaagtcc gacaggtacc
      961 attatgtgag atgcattcga agtcagaagc agggattcgg gcgataaata attatatgga
     1021 tgtgcagttt ttaagttcag gattttcggt ctccgtaaac ccttgacggc agcaggtaat
     1081 gaacagaata tctgtctttg cgcaaggtga ttaaatcagc tgcaaaaaaa tgtacggatc
     1141 tgtcaaggaa
//
LOCUS       DAOQGJ010001732         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105386_length_1149_cov_2.617002, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001732 DAOQGJ010000000
VERSION     DAOQGJ010001732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105386_length_1149_cov_2.617002"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PK683_21070"
     CDS             complement(<1..1026)
                     /locus_tag="PK683_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HNH11002.1"
                     /translation="MIFGADYYPEQWTEKDWDEDFAVMKKMGLTAVRMAEFAWAVFEP
                     GENVFSFSFFDRIIEKAEKAGIKVILGTPTATFPPWLFKKHPDIVQVTKNGIRRIIGT
                     RRCASFHSDEYLKACSRIVKALAEHYGSHPNIIGWQIDNEIGHEGSDLDYSENALKKF
                     RIWLKDRYGEISVLNQNWGGSFWGIIYNDFDEIHLPADHIASGFNPGMIQDFCRFSSD
                     AAVSFINMQADILRTYIKSQFLTVNLYPSPFGNVIDMAELCRKMDFVSWDNYPVWGSQ
                     TEPMPHPFISGCCQYVRGLKDRNFTVMEQISGFQGHTLLGYLPAPGQISLWAANAAFN
                     GAENMVFF"
BASE COUNT          309 a          263 c          228 g          349 t
ORIGIN      
        1 gaaaaacacc atattttcgg ctccgttgaa tgctgcattc gccgcccaga gagagatctg
       61 tcctggagca ggaagatacc caagcagagt gtgtccctga aatcctgata tctgttccat
      121 tacagtaaag tttctgtctt taagtcctct tacatactga cagcatcctg aaatgaaagg
      181 atgaggcatc ggttctgtct gacttcccca tacgggataa ttatcccatg atacaaaatc
      241 catcttcctg cagagttccg ccatatcgat tacattgccg aagggggagg ggtaaagatt
      301 taccgtgaga aactgcgatt ttatatatgt acgcagaata tctgcctgca tatttataaa
      361 gcttactgca gcgtcgctgc tgaacctgca gaaatcctga atcatccccg gattgaaacc
      421 gcttgcgata tgatcagccg gaaggtgaat ttcatcaaaa tcattataaa taattcccca
      481 gaaacttcct ccccagttct gattaaggac actgatttca ccgtatctgt ctttcagcca
      541 gatcctgaat tttttcagag cgttttcgga ataatccaga tctgaacctt catgaccgat
      601 ttcgttgtct atctgccagc cgatgatgtt cggatgtgac ccatagtgtt ctgccaatgc
      661 ttttacaatt ctgctgcagg ctttcagata ttcgtctgaa tgaaaacttg cacatcttct
      721 tgtgcctatt attctccgga ttccgttctt tgtcacctga acgatgtcag gatgcttttt
      781 aaagagccat gggggaaaag tggcagtagg agtgcccaga atgactttaa ttccggcttt
      841 ttcagctttt tcaattattc tgtcaaaaaa actgaagctg aaaacatttt ctccgggttc
      901 aaagactgcc caggcaaact cagccatccg gactgcagtg agccccattt tcttcattac
      961 agcgaagtcc tcatcccagt ctttttctgt ccactgttca ggatagtagt ctgctccgaa
     1021 tatcataaca gctaatatga aaaattcctg tactgagtca agatggttta ttttcagcaa
     1081 ttgagagaga gttttttcca aggaaaatca gcgggaaaac tttcagtttt taattatttc
     1141 cgtacttgc
//
LOCUS       DAOQGJ010001733         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105424_length_1149_cov_2.175503, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001733 DAOQGJ010000000
VERSION     DAOQGJ010001733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105424_length_1149_cov_2.175503"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PK683_21075"
     CDS             complement(<1..978)
                     /locus_tag="PK683_21075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11003.1"
                     /translation="MNTFQGDVMKIHYNKIKIEFLFLILRLILIIPIYFFILPISAEE
                     QKRTAPEKAEEKKDFQKFKYFSFSPGVNLQSADFVVKNGGRSADMTGKDGIISYSLDL
                     KSRDFQISENSGIALFLYNGSIHLDKQYVSDPFSGSLSSSSGSSKKVMDLGTDVNIRT
                     SSFIPSVYFGKKDYASWRLGIGAGYANMNVTGTVGFEDPQEYAFLAFRGNRQNLLNAM
                     TYKYFNSGVIDPNNSDSVLNYLYLNLNQPGSLEGLAAYMGAKGLLKFSSSDVVNYMLF
                     KSAFGDILSPSELLAFAVLSRTNVSHRKTNAPAFKLYLEVPLHSNLLLRV"
BASE COUNT          364 a          240 c          221 g          324 t
ORIGIN      
        1 aactctgagc agaaggtttg aatgcagagg cacttcaaga tacagtttga atgcaggagc
       61 attggtcttt ctgtgagata cattggttct gctgagaact gcaaaagcga gcagttcgga
      121 tggacttaaa atatctccga atgcggactt gaaaagcata taatttacga catcggaaga
      181 actgaacttc agcagtcctt ttgctcccat atatgcagcg agaccttcaa gactgccagg
      241 ctgattcaga ttcaaataca gataattcag gacagaatcg gaattgttcg gatcaataac
      301 ccccgaattg aaatatttgt aagtcattgc attgagaaga ttctggcggt ttcctctgaa
      361 agcaagaaag gcatattcct gaggatcttc aaagcctact gttccagtga cattcatatt
      421 agcataccct gcacctatgc ccagtctcca tgatgcataa tctttttttc caaaatagac
      481 ggaagggatg aacgaagatg tgcggatatt cacatcggtt cccagatcca taactttttt
      541 ggaactcccg gaagaagaac tgagagaacc tgaaaacgga tcagaaacat actgcttgtc
      601 cagatgaatt gagccgttgt ataaaaacag tgcaatgcct gaattctccg aaatctgaaa
      661 atctctggat ttcagatcga gagaataact gataattcca tccttgcctg tcatatcagc
      721 agatcttccg ccattcttca cgacaaaatc cgcactctga agattgaccc cgggactgaa
      781 ggagaaatac ttgaacttct gaaaatcttt tttctcctca gctttttctg gagcagtcct
      841 cttctgctct tcagcagaaa tcggaagaat aaaaaaataa atcggtatga taagaataag
      901 tctgaggata aggaacagaa attctatttt tattttatta taatgaattt tcatcacatc
      961 accctggaat gtattcactc tctttacaga atcaatcgtc taatctgcac gaaacgccgg
     1021 atcagtttca ttatgaatta atgatattac accgaccaag cattttagat ctgccctcgt
     1081 cgcagatact cgaaaaacac ttggcagaag cgaagcaaat tattgctgca actgcttttt
     1141 cataatttt
//
LOCUS       DAOQGJ010001734         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105703_length_1147_cov_2.920330, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001734 DAOQGJ010000000
VERSION     DAOQGJ010001734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105703_length_1147_cov_2.920330"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="PK683_21080"
     CDS             complement(<1..>1147)
                     /locus_tag="PK683_21080"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNH11004.1"
                     /translation="VQAAEGENHIQTAESLKSEEPHLYIAGYYRKEGKENLALYGQVY
                     NTKTGRIVSAFNLTHEDISKLGIRLPEDEMKESDSTVIKKFSEKMALSASINPNGKSE
                     NERIDEYIFQSSIGKDIRFAADREEAAAAAESVFDLLNQTTVSSTKTAKNTRDVPNIV
                     STVSGKEIIDYGRISINDVLYQLPGFAPSQDYDRRTVSSRGTFENWNNNHYMFLMDGV
                     QFNDNQYGTAYTWEVTPLNMIKSLEVIRGPGSALYGSNATNGVITLNTYSGEDFMGKI
                     QTRARAGSQGTQILDISTGNKGKLFSYFTSYSSYQTDGNNYKSYDASGRGWDTGMGFN
                     LLQQFKINDERKNDYLFLKLEGEDFLKGFSVQYHKQKWNYQTGLGWL"
BASE COUNT          265 a          264 c          245 g          373 t
ORIGIN      
        1 aaaagccagc ccagccctgt ctgataattc catttctgct tgtgatactg gacggagaaa
       61 cctttcagga aatcttcgcc ttccagtttc aggaagagat agtcattctt tcgttcgtca
      121 tttatcttga actgctgaag aaggttgaaa cccatccctg tatcccatcc tctgccggag
      181 gcatcgtaac ttttatagtt gtttccgtct gtctggtatg aagagtagga tgtgaaatag
      241 gaaaacagct ttcctttgtt tccggtggaa atatccagta tctgggttcc ctgcgaccct
      301 gctcttgccc tggtctgaat tttgcccata aagtcttcgc cggagtatgt attcagcgta
      361 atgaccccgt ttgtggcatt tgatccgtac agggctgaac cgggacctcg gatgacttcc
      421 agtgatttta tcatattcaa tggagtcact tcccacgtgt atgcagttcc gtactgatta
      481 tcattaaact gtacaccatc catcaggaac atatagtggt tgttgttcca gttttcaaag
      541 gttcctctcg acgagactgt tctccggtcg taatcctgtg aaggagcaaa cccaggaagc
      601 tgatacagca catcgttgat gctgattctt ccgtaatcga taatttcctt tccggagacg
      661 gtgcttacaa tattgggaac atctctggtg ttttttgcag ttttagtgga agaaacagtc
      721 gtctgattca gaagatcaaa tacgctttcc gcagctgctg cggcttcttc tctgtctgca
      781 gcaaatcgga tgtcctttcc gatggaagac tggaaaatat attcgtcaat cctctcattc
      841 tcagattttc cgttcggatt gatgctggca ctcagtgcca ttttttcaga gaatttttta
      901 atgacggtac tgtctgattc tttcatttca tcttccggaa gcctgattcc cagtttggaa
      961 atgtcttcat gagtcaggtt gaatgcagat acaattctgc ctgtctttgt attgtaaacc
     1021 tgaccgtaca gggcaagatt ttcctttcct tcctttctgt aatagccggc aatataaaga
     1081 tgaggttctt ccgatttcag cgattcggca gtctgaatat gattttcccc ttccgcagcc
     1141 tgaacat
//
LOCUS       DAOQGJ010001735         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105712_length_1147_cov_2.785714, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001735 DAOQGJ010000000
VERSION     DAOQGJ010001735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105712_length_1147_cov_2.785714"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="PK683_21085"
     CDS             complement(<1..>1147)
                     /locus_tag="PK683_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNH11005.1"
                     /translation="QEDEFSTVATVTVPKAKESEDKDSVAPKISIFDDFGKEEPISIP
                     FAETKGSLLEKIHSSPFTEEDTGSMEAVALTDENWEEKKLEGDSPDLITQEELQELYN
                     FNEQETSDAEVQADNFFDRMMEAEEENISSENIYSSDPGEEENTAYLSMPAEKPEQPK
                     LPFIRTTIPEVAPLRRAQYKISQKIFTSHQNAKPNPMFKMEAEYVSMKIQQIIKEYGY
                     ESKMEGWEKGPIITRYELTPPPGVKLGRITSLADELKLYLAVKSIRIVAPIPGKSTIG
                     IEVPNKYRENVYLGDILGQKSIDENNPKDLTIAIGKDSISGEIVKIDLNKMPHLLVAG
                     TTGSGKSVCMNSMISYLIFTKSPDEVRFVMIDPKMVELSRFNGLPHLL"
BASE COUNT          241 a          246 c          260 g          400 t
ORIGIN      
        1 caagcagatg tggcagtcca ttaaaacggc tgagttccac cattttggga tcaatcatta
       61 cgaaacgcac ttcatcggga gactttgtaa agatcagata actgatcatt gaattcatac
      121 agacagattt ccctgaacct gtagtacctg ctacgagcag gtgaggcatt ttgttcaggt
      181 cgattttaac tatctctcct gagatactgt cctttccgat ggcaattgtc agatccttcg
      241 gattgttttc atcaatggat ttctgaccga gtatgtcgcc taagtacaca ttttctctgt
      301 acttgttcgg cacttcgatt cctattgtgg attttccggg aatcggcgca acgatacgga
      361 tgctttttac tgcaagatac agtttcagtt catctgcaag ggaagtgatt cttcccagtt
      421 tgactccagg aggaggggtg agttcatacc ttgtgatgat cggtcctttt tcccatcctt
      481 ccattttgga ttcatagccg tattccttga tgatctgctg tattttcatg gaaacatatt
      541 ctgcttccat cttgaacata ggattcggtt ttgcattctg gtggcttgta aatatttttt
      601 gtgatatttt atattgcgct ctccggagag gagccacctc gggaatggtt gtgcgtatga
      661 aaggcagttt cggctgttcc ggtttttctg ccggcatgga aaggtaggca gtgttttctt
      721 cttcgccggg atctgaagag taaatattct ctgaactgat gttttcctct tctgcttcca
      781 tcatcctgtc gaaaaaatta tctgcctgaa cttcggcatc ggatgtttcc tgttcattga
      841 aattgtacag ttcctgaagt tcttcctgag tgatcagatc gggggaatcg ccttccagtt
      901 ttttctcttc ccagttttca tccgtcagtg caactgcttc catggaaccg gtgtcttctt
      961 ctgtgaaagg ggagctgtgt attttttcga gaagggatcc tttggtttct gcaaatggaa
     1021 tgctgatcgg ttcttctttt ccgaaatcgt caaatatgct gattttaggg gctacgctat
     1081 ccttgtcttc ggattccttt gcctttggaa ctgttacagt ggcgactgtg ctgaattcgt
     1141 cctcctg
//
LOCUS       DAOQGJ010001736         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105716_length_1147_cov_2.709707, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001736 DAOQGJ010000000
VERSION     DAOQGJ010001736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105716_length_1147_cov_2.709707"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PK683_21090"
     CDS             <1..206
                     /locus_tag="PK683_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11006.1"
                     /translation="LIRTGQYKPWQLDLRVFSFFLGLLLAFVGAGLSLVFIIVLGKSL
                     AMLGGLIPFAIGLSLLSFYKLQK"
     gene            301..>1147
                     /locus_tag="PK683_21095"
     CDS             301..>1147
                     /locus_tag="PK683_21095"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HNH11007.1"
                     /translation="MNFFRTSSAETAVFQKSDLHLNEPYFSFAEFSGLTLENLNAVSE
                     IREMLNSKIESVTDLHYEYILNSKALKKIIDDHSTLEKLKKTFREYLKSLFSGKIDEE
                     YCRKRQIIGTVHYNIKLYPEWYIGAQGRILDVLIPMLTDRIRDRKKLSFALVSLNKLI
                     HFDSQIILKSYSEMFTYRMNETLCSAVERTADSRNIEAVVNTKEKIQNTVNTLEKIKS
                     NMDLSRNDWKKSEKEFTFLLQESDRSLLDMKITRDRISESLSSIEKISSSYRELVKNW
                     SGLNRE"
BASE COUNT          399 a          208 c          218 g          322 t
ORIGIN      
        1 aactgatcag aacaggtcag tacaaaccat ggcagcttga tttgagagtt ttctcttttt
       61 tcctcggtct gcttctcgcc tttgtcggag caggactgag tctcgtattc attatagtat
      121 taggcaaatc actggcaatg cttggaggtc tcattccatt tgccattgga ctgagtctcc
      181 tgagttttta taaactgcag aaataaaagc ctatcaaaat ctcaaactaa tacacatttt
      241 caaattccac tgcaggcttt tgcttgaaca gattcagaaa aacgatcaga aattagctga
      301 atgaattttt tccgcacatc atctgcagaa actgcagtct ttcaaaaatc agatttacat
      361 ttgaatgaac cgtatttttc ctttgctgaa ttttccggtc tcactctgga aaacctgaat
      421 gctgtttctg aaatcaggga gatgttaaat tcaaaaattg aatctgtgac agatcttcat
      481 tatgaatata ttctgaacag caaagcattg aagaaaatca tagatgatca ttccacgctt
      541 gaaaaactga agaaaacatt cagagaatat ttaaaatccc tgttttcagg aaaaatagat
      601 gaggaatact gcagaaagag acagattatc ggaactgttc attataatat aaaactttat
      661 cctgaatggt atataggtgc tcagggcagg attcttgatg tgctgatccc gatgctgact
      721 gaccggataa gagacagaaa aaagctttca tttgcattag tgtccttgaa taaactgatc
      781 cattttgatt ctcagatcat actgaaaagt tattcggaaa tgttcactta cagaatgaac
      841 gaaacgttat gctctgcagt ggaaagaacg gccgactcaa gaaatataga agcagtagtc
      901 aatacaaaag aaaaaatcca gaacaccgtg aacactctcg aaaagataaa atcaaatatg
      961 gatctttcga ggaatgactg gaaaaaatcg gaaaaagaat ttacatttct tctgcaggaa
     1021 agcgacaggt cacttcttga catgaaaatt acgcgggaca ggatttcaga atcattatcc
     1081 agtatagaaa aaatatcctc aagctacagg gaactggtca agaactggag cggccttaac
     1141 agagaaa
//
LOCUS       DAOQGJ010001737         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105789_length_1147_cov_2.008242, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001737 DAOQGJ010000000
VERSION     DAOQGJ010001737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105789_length_1147_cov_2.008242"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PK683_21100"
     CDS             complement(<1..306)
                     /locus_tag="PK683_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HNH11008.1"
                     /translation="MKCFYCGFENPENIKYCGQCGNVISGPTGTSSGKDADIDALNQL
                     LFPKFLIEKKIGKGGMATVYLGEQTALQRKIVVKILNIDISSDEEIRERFLLEARTPA
                     "
     gene            complement(342..731)
                     /gene="gcvH"
                     /locus_tag="PK683_21105"
     CDS             complement(342..731)
                     /gene="gcvH"
                     /locus_tag="PK683_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNH11009.1"
                     /translation="MEGKKVLDGLKYTEKHEWVKMDGSTAVFGITDFAQNALGDLVFI
                     DLPKVGKQIKKGDSCGTVESVKAAEDVYSPIGGEVAEINSEVTKNPALVNSNPYDSWM
                     VKLKNPASADYDPLMSADKYREYISSL"
     gene            complement(827..>1147)
                     /locus_tag="PK683_21110"
     CDS             complement(827..>1147)
                     /locus_tag="PK683_21110"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HNH11010.1"
                     /translation="AVFLLEVESDESANSERLLKTQIQYKNSKLQTEKQEIEIQYLKK
                     SIWESSENMRQAAGAAAFGLTLKNSLEKGKADRYMAVHILESMPNDRAGKRQDFLKAV
                     LRTE"
BASE COUNT          268 a          265 c          215 g          399 t
ORIGIN      
        1 ggcaggagtt ctggcttcga gcagaaatct ttctctgatt tcttcgtcag aggatatgtc
       61 tatgttcaga attttaacta ctatctttct ctgaagcgca gtctgttcgc ctagataaac
      121 agttgccatt ccgccttttc cgattttttt ctctatcaga aatttcggga ataacagctg
      181 attgagcgca tcaatgtcag catccttgcc ggatgaagtt cctgtgggac cggatatgac
      241 gttaccgcat tggccgcaat attttatatt ctccggattt tcaaatccgc aataaaaaca
      301 tttcattctt tatcagctgt cctgttgttt ctttctatat gtcaaagact ggaaatatac
      361 tctctgtact tgtctgcact catcagtgga tcgtaatccg ccgatgcagg attcttcagt
      421 tttaccatcc atgaatcgta aggattggaa ttgaccaatg cgggattttt tgtgacttcc
      481 gaattgattt cggcaacttc accgccgatc ggagagtata catcctcagc cgccttgact
      541 gattctacag tcccgcagct gtcacctttt ttgatctgct ttccgacttt agggagatca
      601 ataaatacaa gatccccaag tgcattttgt gcaaaatctg tgattccaaa cacagctgtg
      661 gagccgtcca ttttgaccca ctcgtgtttt tccgtatact tcagtccatc cagtactttt
      721 tttccttcca ttcctatacc cattttcaag gtaaattacg ggggataaac ggtcaagaga
      781 gattggattt cagtcattaa agagggcttt tttttggcac agatttttat tccgttctca
      841 ggacagcttt cagaaaatcc tgtcttttcc ccgctctgtc attcggcata ctttccagaa
      901 tatgcactgc catatatctg tccgcttttc ctttttccag agagtttttc agagtcagtc
      961 caaatgcagc ggcacctgca gcctgtctca tattttctga actttcccag atggactttt
     1021 ttaaatactg aatttcaatt tcctgttttt ctgtctgaag ttttgagttt ttgtactgaa
     1081 tctgagtttt cagaagcctc tctgaatttg cggattcgtc tgactccact tcaagaagga
     1141 atactgc
//
LOCUS       DAOQGJ010001738         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105938_length_1146_cov_2.369386, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001738 DAOQGJ010000000
VERSION     DAOQGJ010001738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105938_length_1146_cov_2.369386"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK683_21115"
     CDS             <1..318
                     /locus_tag="PK683_21115"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNH11011.1"
                     /translation="GLGKGARPMELLLMGLASCSAIDIISILKKQRQTVRDFTADVNG
                     EREQGKEASVFTAIHIHFKAYGEIEKDRLETAIHLSLDKYCSAAKTLEKTAKITHSYE
                     ILP"
     gene            complement(552..974)
                     /locus_tag="PK683_21120"
     CDS             complement(552..974)
                     /locus_tag="PK683_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11012.1"
                     /translation="MKKILALSFILFLLNCTKSAEVTQEGAGFYADEKLQTKIADLSK
                     GTVVEALDYRNHAWNVRDSIKIKYNSQVGYVSPRSLVVGQNPEKSVFKWGYRADYKPF
                     LDPNDKTRYKHGFEYPQLKNLPKTKVPLEQLLEGAKLD"
BASE COUNT          348 a          257 c          221 g          320 t
ORIGIN      
        1 gggctgggaa aaggtgcaag accaatggaa cttcttctga tggggcttgc ctcctgcagt
       61 gcgatcgata taataagcat acttaaaaaa cagagacaga cagtcagaga ttttacagct
      121 gatgtgaacg gtgaaagaga acagggcaag gaagccagcg tattcactgc aattcatatc
      181 catttcaagg catacggtga aatagaaaag gacagactgg aaacggcaat ccatctgtcc
      241 ctcgacaaat actgttcagc tgcgaagact ttagaaaaga ccgcgaagat tactcacagt
      301 tatgagatcc tgccttaact cttcagctct tatttttccg gattcaccct taccgtcaca
      361 tcctgtttgt atgcaattgc accaagcata taaatccata cgccgctgct tgaattcggc
      421 tgtccataca ttttgccata ataataagga cacagctaaa cagaattttc actttcaatg
      481 tctctccttg cataaaaaag ccccgtcata ctgtgtatca cgaggctttt caatttttga
      541 ttgaggaaaa ctcagtcgag ttttgcacct tccagaagct gttccagagg aacctttgtc
      601 tttggcaggt tcttcagctg cggatattca aaaccatgtt tgtatctggt cttgtcattt
      661 ggatccagaa acggcttgta atctgctctg taaccccatt taaaaacaga cttctcagga
      721 ttctgaccga caactaaaga tcttggagat acataaccca cttgggaatt gtacttgatc
      781 tttatactgt ctctgacatt ccaggcatgg tttctgtagt ccagcgcttc aacaacagta
      841 cccttagaaa gatctgcgat tttagtctga agcttctcat cagcataaaa cccagcacct
      901 tcctgagtca cctctgcact cttagtgcag tttaaaagga aaagtataaa acttaatgca
      961 agaatctttt tcatataatc tccttactga tgatgattca gacactgttc cagtctagga
     1021 tctccgacag gaacaagctt agccttctga agtttccaga gaataacaaa tccgaatact
     1081 ccgaaagttc caatggttac acctaaagaa agaatgaaac tgagaggatt gaatgtctcg
     1141 aaattg
//
LOCUS       DAOQGJ010001739         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_105975_length_1146_cov_1.980752, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001739 DAOQGJ010000000
VERSION     DAOQGJ010001739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105975_length_1146_cov_1.980752"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1146)
                     /gene="priA"
                     /locus_tag="PK683_21125"
     CDS             complement(<1..>1146)
                     /gene="priA"
                     /locus_tag="PK683_21125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNH11013.1"
                     /translation="VPLKNRKESLEGVVLSIGNSMPAYECREITRLVDKNPVINGEQI
                     ELAYWMKEYYLASLGECLYKMIPSGRRNKSSEPDIESFSELLSLNEEQKSAYEKVKSS
                     FGTDSIHLLHGITGSGKTEVYIHLLNDLLKNTDKSAILLVPEISLTFQILKRLQMVFG
                     NALAILHSGLRISEKFQNYMQLQRGEKRIAVGTRSGVFAPVRNLGLIIIDEEHDFSYK
                     EHSSPRYHARQIAMQRCRTAKAPLLLGSATPSIESYYFALKGKIILHELKKRAVSASL
                     PTVRIIQKKEDSEVISSELLFQLKKRLERKEQTVILLNRRGHSPLLYNRETRKFIECP
                     NCTSNLCFHKKGTVICHLCGYTEKFAVLQKRMKTELELLGAGTQKLEE"
BASE COUNT          263 a          249 c          263 g          371 t
ORIGIN      
        1 ctcttcgagt ttctgagtgc ctgcgcccag aagttccagt tctgttttca tccttttctg
       61 cagaacggca aatttttcag tatatccgca gagatgacag attacagttc cctttttatg
      121 aaagcagaga ttggatgtgc agttcggaca ttcaataaat ttcctggtct ctctgttgta
      181 cagaagaggg ctgtgtccgc gtctgttcag aaggatgact gtctgttcct ttctttccag
      241 tctttttttc agctgaaaca gaagttctga actgatgact tcagagtctt cttttttctg
      301 aatgattctg actgtcggca gggatgctga tacagctctt tttttcagtt cgtgcagaat
      361 gatttttccc ttcagtgcaa aataatagga ttcaatggag ggagttgctg atccaaggag
      421 aagaggggct ttggccgttc tgcatctctg cattgcaatc tgtctggcat ggtaccgggg
      481 agagctgtgt tccttgtagg aaaaatcatg ctcctcatca atgatgatga gtcccagatt
      541 tcttacagga gcaaagactc cgctccttgt gcctactgcg attctttttt ctcctctctg
      601 gagctgcata tagttctgaa atttttcaga aatccggaga ccggaatgaa ggatcgccag
      661 tgcgtttccg aaaaccatct gcagtctttt cagtatctga aatgtcagac tgatttccgg
      721 tacaagaagg atggcagact tgtctgtgtt tttcagaaga tcgttcagaa gatgaatgta
      781 gacttccgtc tttccgcttc ctgtaattcc gtgcagaaga tgaatggaat cagttccgaa
      841 agaactcttc actttttcgt atgctgattt ctgttcctca ttgagtgaaa gcagttccga
      901 aaacgattct atgtccggct cggaggactt gtttcgtctg ccggaaggaa tcatcttgta
      961 gagacattct cccagcgagg ccagatagta ttccttcatc cagtaggcaa gttcaatctg
     1021 ctctccattg atgacagggt tcttgtccac aagtctagtg atttccctgc attcgtatgc
     1081 gggcatactg tttccgatgg aaagtacgac gccttcgaga ctttcctttc tgtttttcag
     1141 aggaac
//
LOCUS       DAOQGJ010001740         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_106389_length_1143_cov_4.295037, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001740 DAOQGJ010000000
VERSION     DAOQGJ010001740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106389_length_1143_cov_4.295037"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PK683_21130"
     CDS             <1..979
                     /locus_tag="PK683_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HNH11014.1"
                     /translation="DGSGNIEGFFVFAGGGMGMTHNKPETYPRAADLLGWIPKEGLIP
                     AAEAIVTAHRDFGDRTNRKHARLKYVLQEKGVDWFRSEVESRSKVKFVQKTLPEWKSP
                     SILGWIPAEDGTYSLGFHTLAGRLKDTPGKPLKTALKEIIKKYNLSVQISPDQDMILM
                     DISKEAKPEIEKSLSAYGIEYRSPKPLYDRALACPALPTCGLALTESERSFPSLLSEL
                     QKVLDRHGLSEKAPLLRMTGCPNGCARPYSAEVGIVGQQSGGKYAVYAGGDPEGTRVG
                     FQIASKIPQAEIPALLEKIFVHWKSEKPEISFGDYVFSKGKDAVTEMLK"
BASE COUNT          362 a          242 c          254 g          285 t
ORIGIN      
        1 ggacggctcc ggaaatattg aaggtttttt tgtgtttgca ggcggcggaa tgggtatgac
       61 tcacaacaag ccggaaacat atcccagagc agcagacctt ctcggctgga ttccaaaaga
      121 aggactgatc cccgcagcag aagcaattgt aactgcacat agagatttcg gagataggac
      181 aaacagaaaa cacgccagac tgaaatatgt ccttcaggaa aaaggtgttg actggttcag
      241 atccgaagtg gagagcaggt caaaggtcaa gtttgtgcag aagactcttc cggaatggaa
      301 aagtccttct atccttggat ggattccagc agaagacgga acctattccc ttggctttca
      361 tacactcgca ggaaggctga aggacacacc cggcaaaccg ctgaaaaccg cattaaaaga
      421 aattataaaa aaatataatt tatctgttca gatttccccc gaccaggata tgattcttat
      481 ggatatttcc aaggaagcga aaccggaaat agaaaaatca ctttctgcat acggaattga
      541 gtatagaagc cctaaaccgc tttatgacag ggcactggcc tgccctgctc tccccacatg
      601 cggacttgcc ctcaccgaaa gtgagagatc ttttccaagt ctcttgagcg aacttcagaa
      661 ggtcctggac agacacggtc tttcagaaaa agcccctctt ttaagaatga ctggatgccc
      721 taacggatgt gcgagacctt actctgctga agtaggaatt gtaggtcagc agagcggagg
      781 caaatatgca gtctatgcag gcggtgatcc ggaaggaaca agagtgggat ttcagattgc
      841 atccaaaatt cctcaggctg aaattccggc actccttgaa aaaatatttg ttcattggaa
      901 atcggaaaaa ccggagattt cattcggcga ctatgtcttt tcaaaaggaa aagatgcagt
      961 cactgaaatg ctgaaataat ctgcttcaaa ttttactgta ctggaattta tcagaattcc
     1021 agtacagatt tactaaaact cctgcgcata aactgaaaaa atactttatc atgcaaaaat
     1081 cattataatc attttcataa ataattgcgc tttttccggc tgacagactt gattttactg
     1141 agt
//
LOCUS       DAOQGJ010001741         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_106637_length_1142_cov_2.559338, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001741 DAOQGJ010000000
VERSION     DAOQGJ010001741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106637_length_1142_cov_2.559338"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1142
                     /locus_tag="PK683_21135"
     CDS             <1..>1142
                     /locus_tag="PK683_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11015.1"
                     /translation="VNNGSKAEFAVSPEESLLDKNVKLQAIEDGNRESETVSILISAS
                     GYPDLEVSLKINDLYARKVSLTLPDTTAEGISANLFVSLGIKPESNVSVTLTASDPSV
                     SFPSGSSLTFTKENYDTIQSVQFTINDIFNDSRSYSITAVSDLENNTKSTLVEDNDFA
                     EIDISGSVLQGTGSGSHPSVLMDDVNGKLLTATYNQSNAGKPSVYRCSMDGSGCIHYD
                     VSSGMGLDSGRRPNLNWDKNADKLVLFSMQKNVFKCDTDVTNCSSLGGIASNLYADFP
                     YLIYPSASSSNVIVKDIRSVFYCDGNFSICSVDSANPSNFASYAYGSRRGIIDSLNSK
                     IITSHTNVSTYAPRIVRWDLNSVTNPVPIDLSSVTNSAISTDLVID"
BASE COUNT          327 a          240 c          232 g          343 t
ORIGIN      
        1 ctgtaaacaa tggctccaaa gcagagtttg cggtgtctcc cgaagaatca ctcttggata
       61 agaatgtaaa acttcaggcg attgaagacg gaaaccgaga gtcagagact gtcagtattc
      121 tgatcagtgc ttctgggtat ccggatctgg aagtctcatt gaaaattaat gatctgtatg
      181 ccagaaaagt cagtctcact ttgcctgata caactgcgga ggggatctca gcaaatcttt
      241 ttgtttccct gggaatcaag ccggaatcca atgtttcagt caccttgacg gcatctgatc
      301 cttcagtttc ctttccctca ggttccagtc ttacctttac aaaagaaaac tatgacacaa
      361 tacagtctgt tcaatttact atcaatgata ttttcaatga cagtcgctcc tattccatta
      421 ctgcagtttc cgatctggaa aacaatacaa agtctactct tgtggaggac aatgattttg
      481 cagaaattga tatttccggt tctgttctgc agggcacagg ttcaggaagt catccttccg
      541 ttctgatgga tgatgtgaac ggaaaacttc tgacggccac atacaatcag tcgaatgcag
      601 gaaaaccatc cgtttacaga tgcagtatgg acggttcagg atgtattcat tacgatgtat
      661 cctccggaat gggactggac tcaggcagaa gaccgaatct caactgggac aaaaatgcag
      721 acaaacttgt acttttttct atgcaaaaaa atgtattcaa atgtgatact gatgtgacaa
      781 actgttccag cctgggagga atagcatcga atctttatgc tgatttcccg tatttaatct
      841 atccttctgc aagttccagc aacgtcattg taaaagatat ccgttctgtg ttttactgtg
      901 acggtaattt ttctatatgt tcagttgatt cagccaaccc ttcaaatttt gccagctatg
      961 catatggaag cagaagagga atcattgaca gcctgaattc taagattata acatctcata
     1021 ctaatgtctc tacttacgct ccgagaatag tccgatggga tttgaacagt gtcactaatc
     1081 ctgtgccgat tgatctgtcc agtgttacaa attctgcaat ctctacagat ttggtcattg
     1141 at
//
LOCUS       DAOQGJ010001742         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_106718_length_1142_cov_1.487580, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001742 DAOQGJ010000000
VERSION     DAOQGJ010001742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106718_length_1142_cov_1.487580"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PK683_21140"
     CDS             complement(<1..548)
                     /locus_tag="PK683_21140"
                     /inference="COORDINATES: protein motif:HMM:NF018125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HNH11016.1"
                     /translation="MSTRKKRGIARRYDAFIDQNTGRGTERDKLSGISGTNESISPEY
                     ARRLYRSNGFIQTVADSPAEDATKEWIEIETNREDIQASRLIMNRLEDLKIRQKILKL
                     IRYSRMYCEGAVLYYCIKTDIPETDLSKPIENILSLEAVNVVGADRFAVQFQNYDPLH
                     PDYSKPSIVISGREIHPQRYSWL"
     gene            complement(545..>1142)
                     /locus_tag="PK683_21145"
     CDS             complement(545..>1142)
                     /locus_tag="PK683_21145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HNH11017.1"
                     /translation="GAIYQQEPVDIQEKLFTEPKLEECPEGIKLYAYLDPALGGSDYS
                     ALTVGGVETKNGITKIFIRAAGLWRVQLDEMYSKVEQMCRMHKVSVLYVESNQAQKAI
                     ALEFTKRSLGVKEVHNTQNKYLRIMNAVKLNWDKIRFGAGVPGDYMKQILNYTELSAH
                     DDAPDSLAGLIEAIHISKDEKFRNRYNFSSIFKFGRRL"
BASE COUNT          242 a          283 c          247 g          370 t
ORIGIN      
        1 agccaggaat atctctgagg gtgaatttct cttccggata tgacgatgga cggcttgctg
       61 tagtcaggat gaagaggatc ataattctga aactgcacag cgaatctgtc tgctcctaca
      121 acgttcactg cctcaagact caggatgttt tctattggct tggaaagatc cgtttcgggg
      181 atatctgttt taatgcagta gtagagaact gctccctcac agtacatccg gctgtatcgg
      241 atcagtttga ggattttctg cctgatcttc aggtcttcca gtcggttcat aatcagtctg
      301 gatgcctgga tgtcctcccg gtttgtctca atctcgatcc actctttagt cgcgtcctct
      361 gcaggtgaat ccgcaacggt ctgaatgaat ccgttggatc tgtaaagccg tcttgcatat
      421 tccggggaga tgctttcatt ggttcctgag attcctgaga gtttgtccct ctctgttcct
      481 cttcctgtat tctgatctat aaatgcatcg tatcttctgg ctattcctct tttctttctt
      541 gtgctcacag tcgtctcccg aatttgaaaa tggaggaaaa gttgtatcgg ttcctgaact
      601 tttcatcctt tgaaatatgt attgcttcaa tcagtcctgc cagtgaatcg ggagcatcat
      661 cgtgtgcaga cagttcagtg tagttcagta tctgcttcat atagtctccc ggaactcctg
      721 caccgaaccg tatcttgtcc cagttcaact tcactgcgtt cataattctc agatacttgt
      781 tctgagtgtt gtgcacttcc ttgactccga ggcttctctt tgtgaattcg agtgcaattg
      841 ccttctgagc ctgattggat tctacataaa gcacactgac tttatgcatc ctgcacatct
      901 gttccacttt ggaatacatc tcatccagct gaactctcca gagacctgca gccctgatga
      961 aaattttagt gatgccgttt tttgtctcca caccgcccac cgtcagtgca gagtagtcgc
     1021 tccctccgag tgcaggatcg aggtatgcat agagcttgat tccttccgga cattcctcaa
     1081 gcttcggttc agtgaaaagt ttttcctgaa tgtccacagg ttcctgctga tagattgcac
     1141 cg
//
LOCUS       DAOQGJ010001743         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_106778_length_1141_cov_2.807551, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001743 DAOQGJ010000000
VERSION     DAOQGJ010001743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106778_length_1141_cov_2.807551"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..527
                     /locus_tag="PK683_21150"
     CDS             159..527
                     /locus_tag="PK683_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11018.1"
                     /translation="MIISILLFSAYCREKSGKDHSFLKKFDTKPVLGMWRVVPSMNSE
                     VILFEPDGGVKVFQNSKEEKMYAYSDSRGMRLTRSENDPHAVGFFLFAEKNEGVWNGV
                     MNEKVVRLVRIGEEKKSVLE"
     gene            complement(490..>1141)
                     /locus_tag="PK683_21155"
     CDS             complement(490..>1141)
                     /locus_tag="PK683_21155"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNH11019.1"
                     /translation="QAGKELSLPKRDFTVCLMPGSRSGEIRRLLADMLASASEIRKRL
                     SQENKSVLFLVPSISEKEEKYILDSIEEIRKENPELEIQYHYKNTLRCIEKSDMVILA
                     SGTATLEVACFEKPMVILYKNSMFTHWLGKKLVLIPYVGLVNVLSERFICREFLQEDC
                     NPQNITEESMKILRDEAYRKKMTEDIILVKKSLGDGKASEIAGNSILELISSPLRS"
BASE COUNT          335 a          218 c          238 g          350 t
ORIGIN      
        1 cgaaacctgg aatttaattc agattcctgg tcatggaaag gattcggaaa ttccaccaaa
       61 acaggatata cattttcagg ctggggtgca aactcaggaa aaagccagaa cttcacagtc
      121 aacagggaga aaggaaaatg cgttttacag aaaaaataat gattatttca atactgcttt
      181 tttcggcata ctgcagggaa aaatcaggaa aggaccattc ctttctgaaa aaattcgata
      241 caaaacccgt actgggaatg tggagagtgg ttccttctat gaattccgaa gtcattctat
      301 ttgagcctga cggaggggtc aaagttttcc agaattcaaa ggaagaaaaa atgtatgcct
      361 attcagattc cagaggaatg cgtctgacaa gatcagaaaa tgatcctcat gctgtcggct
      421 tttttctttt tgctgaaaaa aacgaaggtg tatggaatgg tgtcatgaat gaaaaggttg
      481 tcagacttgt caggatcgga gaggagaaga aatcagttct agaatagaat tacctgcaat
      541 ttcagacgct ttcccgtccc ctaagctttt tttgaccaat atgatatcct cagtcatttt
      601 ttttctgtag gcttcatctc tcaggatctt catggattct tcagtaatat tctgaggatt
      661 gcagtcttcc tgcagaaact ctctgcagat aaatctttca gaaagaacat ttacaaggcc
      721 tacataagga atcagcacaa gttttttccc cagccaatga gtgaacatac tgtttttgta
      781 gagtatcacc atcggttttt caaaacaggc aacctccagt gttgccgttc ccgaggcaag
      841 tatgaccata tccgattttt ctatgcaccg gagagtgttt ttataatgat attgtatttc
      901 gagctcagga ttttcttttc tgatttcttc tatcgaatcc agaatgtatt tttcttcctt
      961 ttcactgatc gaagggacaa gaaaaaggac tgatttattt tcctgtgaaa gtctttttct
     1021 gatttcagag gcggatgcaa gcatatctgc gagaagcctt ctgatttctc ctgatctgga
     1081 tccgggcata aggcatactg taaagtctct tttgggaaga gagagttcct ttcctgcctg
     1141 c
//
LOCUS       DAOQGJ010001744         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_106790_length_1141_cov_2.646409, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001744 DAOQGJ010000000
VERSION     DAOQGJ010001744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106790_length_1141_cov_2.646409"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PK683_21160"
     CDS             <1..539
                     /locus_tag="PK683_21160"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNH11020.1"
                     /translation="SVFKKHIQDLPILTSLVAFDLTVPLNLKGLRDEIFTLTLLRRIG
                     ATIFAMNFSKRYQDVIKLAEAGVKSIVTIEREEFGTDSIEVGLKVFHQWNMPKSYHEI
                     VKNQDFSILNYTEVSDYDRLTRIADILSRKLYSILVSPEENELLETIFRHYKASSEII
                     DAFGPDYYDNIKDHPYFI"
     gene            complement(527..1078)
                     /locus_tag="PK683_21165"
     CDS             complement(527..1078)
                     /locus_tag="PK683_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNH11021.1"
                     /translation="MELNEKQILIGVTGSIAAYKACELVRNLKKEGFPVRVVMTENAL
                     NFVGRTTFEALTDLPVYSSEYGGGMPHIELKNTAAVFAIVPATANIIGKMANGIADDL
                     LSSLYLAAVCPVIAAPAMNPGMYSHPAVQRNIQQIRKDGVEVIEPDDGTVVCGDTGKG
                     KLAEIPIIQKRIIEVFNNQSYIK"
BASE COUNT          320 a          273 c          219 g          329 t
ORIGIN      
        1 gctcagtctt caagaagcat attcaggatc ttccgatcct gacatctctt gttgcatttg
       61 atctcacagt acctctgaat ctcaaaggac tgagggacga aattttcaca ctcactcttc
      121 tccgaaggat cggggcaacc atttttgcaa tgaacttttc gaaaagatat caggacgtaa
      181 tcaaactcgc agaagctggc gtgaaaagca tagtgacgat agaacgggaa gaattcggaa
      241 ctgactcaat agaggtcggt ctgaaagtat tccatcagtg gaatatgccc aaatcctatc
      301 atgagattgt aaaaaaccag gatttttcaa ttctgaacta tactgaggtc agcgactacg
      361 acagacttac taggattgcc gacatccttt caagaaaact ctattccatt ctggtttcgc
      421 ctgaggaaaa tgaactgctc gaaactattt tcagacatta caaggcctcc tcagagatta
      481 tagatgcatt cggccctgat tattatgaca atataaaaga tcacccttat tttatataag
      541 actgattatt aaatacttct attatccgtt tctgtatgat cggaatctct gcaagctttc
      601 ccttcccggt gtccccgcat acaacggttc cgtcatccgg ttcaatgacc tcgactccgt
      661 cttttctgat ctgctgaatg tttctctgca ctgcaggatg agagtacatt cccggattca
      721 ttgcaggcgc tgcgatcacc ggacagactg ctgcaagata cagcgaacta agaagatcat
      781 cggcaatccc gttcgccatt tttcctatga tattggccgt tgcaggaaca attgcaaaaa
      841 cggccgcagt attcttcagt tcaatatgag gcatccctcc tccatactca ctgctgtata
      901 ctggaagatc ggtaagcgct tcaaaagttg tcctgccgac aaagttcagt gcattttcag
      961 tcatcacaac tcttacagga aaaccttctt ttttaagatt tctgaccagt tcacatgcct
     1021 tgtatgcagc aatcgaaccg gtgacaccta tcagtatctg tttttcgttc aattcaatat
     1081 ctccttgagg ttgtgtaaaa gcatacctcg atacaatttt caccgccggt caccgagtga
     1141 a
//
LOCUS       DAOQGJ010001745         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_106854_length_1141_cov_1.891344, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001745 DAOQGJ010000000
VERSION     DAOQGJ010001745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106854_length_1141_cov_1.891344"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PK683_21170"
     CDS             <1..167
                     /locus_tag="PK683_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HNH11022.1"
                     /translation="LSDYEKKSSALYSSARLWDDGIISQTDTRAVLGTALNLVKDKNR
                     DLKNFGLFRM"
     gene            complement(168..659)
                     /locus_tag="PK683_21175"
     CDS             complement(168..659)
                     /locus_tag="PK683_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11023.1"
                     /translation="MNPVKESSIADIERNGYLGIERTLAPKNVLEMRKEYAETAISLW
                     MHAASSDTPNKHQERKKVLEFIQLLFHEGSLFPPSMYKESEQKNILIDLVKEKEPMTI
                     EEISEYLKGKKELILAFFYDACLLVYLDGKLVSTENDFLDTIAGAWGITKLEKRTTME
                     KFQ"
     gene            complement(662..>1141)
                     /locus_tag="PK683_21180"
     CDS             complement(662..>1141)
                     /locus_tag="PK683_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11024.1"
                     /translation="AERDLKMDFPGEKGEALFSLISAYDRLLKELSELEKNQYLDRFQ
                     KKQKEFELKKLIFGENISSLLFPENDSEKIDLFYRYTERYLKNHFEDSQKDNREHLNL
                     ARKEIYGKNYRELTERESYSMKLDIELRLNEREMSIMNESERKERIAKIKKRLNSEE"
BASE COUNT          289 a          236 c          196 g          420 t
ORIGIN      
        1 tactttccga ctatgaaaaa aaatcatctg cactgtacag ctccgcaaga ctctgggatg
       61 acggaatcat cagtcagact gatacaaggg cagttctggg aactgcactg aatttggtga
      121 aagataagaa cagagatctg aaaaactttg gtcttttcag aatgtaatta ctgaaatttc
      181 tccattgtag ttctcttctc cagtttagtt attccccagg ctcctgcaat cgtatccagg
      241 aaatcatttt ctgtgcttac aagtttccca tccagataga ccagaagaca ggcatcataa
      301 aaaaatgcga gaatgagttc cttttttcct ttcagatact cactgatttc ttctatcgtc
      361 ataggttctt tttcctttac cagatcaatc agaatgtttt tctgttcgga ttctttgtac
      421 attgaaggtg gaaacagaga gccttcgtga aacagaagct gaatgaattc cagaactttt
      481 tttctttcct gatgcttgtt cggtgtatcg gaggatgcag catgcatcca taagctgatt
      541 gctgtttctg catactcttt tctcatttcg agaacattct ttggagcaag agttctttct
      601 attcccaaat agccgtttct ttcaatatcc gctatggagc tttcttttac cgggttcata
      661 tttattcttc cgagttcagt ctttttttaa ttttagcaat tcgttctttg cgttctgatt
      721 cattcataat gctcatttct ctctcattca gacgaagttc aatgtccagt ttcatcgaat
      781 aggattctct ttcggtcaac tctctgtagt tttttccata gatttctttc ctggcaagat
      841 taagatgctc tctgttgtct ttttgagaat cttcgaaatg gtttttcaga tatcgttctg
      901 tatatctgta aaacagatca attttttcac tgtcattctc aggaaaaagc agggaactga
      961 tattttctcc gaaaatcagt tttttcagtt caaattcttt ctgttttttc tgaaatctgt
     1021 ccagatactg atttttctcc agttctgaaa gttccttcaa aagcctgtcg taggccgaga
     1081 tcagtgaaaa aagagcttcc cctttttctc ccggaaaatc catttttaag tctctttctg
     1141 c
//
LOCUS       DAOQGJ010001746         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_106917_length_1140_cov_3.921659, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001746 DAOQGJ010000000
VERSION     DAOQGJ010001746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106917_length_1140_cov_3.921659"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1140)
                     /locus_tag="PK683_21185"
     CDS             complement(<1..>1140)
                     /locus_tag="PK683_21185"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH11025.1"
                     /translation="MYVDGNLISEAGHPGKTKEDTVPDYSPKIARFKCSSSEIDIRIH
                     ISNFHYRTGGFWRNIYLGKAEHLERIRYNENISDLILCGMLFTMGIYHFGIFLLRRSE
                     PASAYFGLFCFFLIFYNMTINDIFLKQVFPEMSFRTLLSIHCFSFTLPIPAMSLFVKR
                     MFPSDFHRPVMIPVYAAVLAAGALILFLPTEIFSQIVQPMRLMIIISSLYLIYLLAKA
                     SLKGNYEAKVFLSGFIIFFISVGNDILRANHILNTEYLSSYGFMFFIMAQSFVLSRRS
                     SEAFRLSGTLARELSLQNITLEQKNRELRMLKSSLEDMVADRTKELLAAKENAELAAN
                     ARYRFLANMSHEIRTPLNSIIGLTDLLLEAELKSEERDMAETVNS"
BASE COUNT          336 a          240 c          253 g          311 t
ORIGIN      
        1 tggaattgac cgtttcagcc atatctcttt cttccgactt aagttcagcc tcgaggagga
       61 gatccgtcag accgatgatg gaattgagag gtgtccttat ttcatggctc atatttgcaa
      121 ggaatctgta tcttgcattt gctgccagtt ctgcattttc ctttgcagca agaagctcct
      181 tcgtcctgtc ggcaaccata tcctcgagag aggatttcag catcctgagt tctctgttct
      241 tctgttccag tgtaatattc tgcaaagaaa gttccctggc gagtgtacct gaaagtctga
      301 aagcctccga ggaccgtctg gacagtacaa aagactgagc catgatgaaa aacatgaatc
      361 cgtaactgct gagatattcc gtattcagaa tatgatttgc cctcaggatg tcgtttccga
      421 ctgaaataaa aaatatgatg aagcctgaaa gaaaaacctt tgcttcataa tttcccttca
      481 gtgaggcctt tgcaagcaga tagatcagat acagtgaaga tatgatgatc atcagtctca
      541 tcggctgaac aatttgagag aatatttctg tgggcagaaa aagaatcagt gccccggcag
      601 caaggacagc tgcatatacc ggaatcatta ccgggcggtg aaaatccgaa gggaacattc
      661 tctttacaaa aagggacatg gctggaatcg gaagcgtgaa tgaaaaacag tggatgctca
      721 gcagtgttct gaaggacatt tcagggaaca cctgcttcag gaatatgtcg ttgattgtca
      781 tattgtagaa aatcagaaaa aagcagaaaa gcccgaaata ggctgaggca ggctcgcttc
      841 tccttaaaag aaaaattccg aaatgatata tccccatagt aaaaagcatt ccacagagaa
      901 tgagatcaga tatgttttca ttatatcgta ttctttccag atgctcagcc ttgccgagat
      961 aaatatttct ccagaacccg cctgttctgt agtggaaatt ggatatatga atccgtatgt
     1021 cgatttcaga tgaagaacat ttgaaccttg caattttcgg cgaatagtcc ggtacagtat
     1081 cctccttggt ctttccagga tgaccggcct ccgagatcag atttccgtcg acatacatcg
//
LOCUS       DAOQGJ010001747         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_106945_length_1140_cov_3.024885, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001747 DAOQGJ010000000
VERSION     DAOQGJ010001747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106945_length_1140_cov_3.024885"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1140
                     /locus_tag="PK683_21190"
     CDS             <1..>1140
                     /locus_tag="PK683_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11026.1"
                     /translation="LFLFCGWHFQHIYPGHGNFFSTPLIVWLAAFFVQYLKKGNISFL
                     FLFSITVCILLSEGNIHIFLYTYLLLLISFLYCLFYDRKSAYRILSASLTGLAFSSYR
                     LLPEAEFLFTTGVFYHPADRNFLNPADLWKIFTASSQHPELSFHFQNQEYRWWEYGNY
                     TGSIPFIVFICLTGFLKRKDIPLILLTVFTALYSLGNFHPLSPANIFSHLPGFSNMRC
                     HSRWTLFTVFFFSIQLGILITRMQIWISEKKRNRGQAWFRYLSYFAVLILCIVLYNDL
                     NRKNAKLVSGIFSIPYSAMNVQKTEFLTVREIRGYGGNSAMLPAILSGISVKEGYETL
                     NRNTNQKSVEDTDYKGEYYLENTDNIRLISKTPSRLSFSVSLREG"
BASE COUNT          291 a          237 c          204 g          408 t
ORIGIN      
        1 actctttctt ttctgcggat ggcattttca gcatatatat ccaggacatg gaaacttctt
       61 ctcgactcct cttattgtct ggcttgcagc attctttgtt cagtatctta aaaaagggaa
      121 tatttccttc ctttttcttt tctcaattac tgtctgtatt ctcctgtctg aaggaaatat
      181 tcatatattt ttatatacgt atttactgct tctgatatca tttttatact gtctttttta
      241 tgatagaaag tctgcttata gaattctttc tgcatcactg accggacttg cattttcttc
      301 ctacaggctt cttcctgaag cagaatttct gttcaccaca ggtgtatttt atcatcctgc
      361 cgacagaaat tttcttaatc ctgctgatct ctggaagata ttcactgcct cctctcagca
      421 tcctgagctg tcctttcatt ttcagaacca ggagtacaga tggtgggaat atggaaatta
      481 taccgggtcg attcctttca ttgtttttat ttgtctcact ggatttttga aaaggaagga
      541 tatccctctg attcttctca ctgttttcac tgctttgtat tcacttggaa attttcatcc
      601 tctttctcct gcaaatatct tcagccatct tccgggcttt tccaatatga gatgtcattc
      661 caggtggact ctattcactg tattcttttt ttcaatccag ctgggaattc tgatcacgag
      721 aatgcagata tggatctctg aaaaaaaaag gaacaggggt caggcatggt tcagatatct
      781 ttcttatttt gctgtcctga ttctctgcat tgttctttac aatgatctaa acaggaaaaa
      841 tgctaaactt gtttccggga tattcagtat cccatattct gcgatgaatg tccagaaaac
      901 ggaatttctg acagttcggg aaattcgagg ctacggagga aattccgcca tgctgcccgc
      961 aattctctcc ggaatctcag tgaaggaagg ttacgagact ctgaacagga atacgaatca
     1021 gaaatctgtt gaagatactg attataaagg agagtattat ttagaaaaca cagacaatat
     1081 cagactgatt tccaaaactc cttcccggct ttctttttct gtcagtttgc gggaaggaaa
//
LOCUS       DAOQGJ010001748         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107125_length_1139_cov_2.951107, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001748 DAOQGJ010000000
VERSION     DAOQGJ010001748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107125_length_1139_cov_2.951107"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PK683_21195"
     CDS             complement(<1..333)
                     /locus_tag="PK683_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11027.1"
                     /translation="MKTLFLYILFFSGCLRMQVDRYWYSPVASLPIRMFPRIETHFDV
                     HQIYGTERIRNIRIGFEKNEKMERGYVFLENGKEFSLSKKSVYIPNSVFIMYLYNEDG
                     TFSEKRFIN"
     gene            complement(436..714)
                     /locus_tag="PK683_21200"
     CDS             complement(436..714)
                     /locus_tag="PK683_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11028.1"
                     /translation="MKKSSVAVKERKKRVNWSDRLKLQTKEVLYDLPVSAFLPGYCCM
                     KSSEHQFGIIRLEDALRNAYIIRSRDSGEIIREFTDVVSLISAGWVVD"
     gene            complement(903..>1139)
                     /locus_tag="PK683_21205"
     CDS             complement(903..>1139)
                     /locus_tag="PK683_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11029.1"
                     /translation="IKYYNPVLDARVLARILKTENQISKMNESGLSLRKFIDSDIQSV
                     KTLYSDVKELRLLFAADIASALNVTGTASSSDGD"
BASE COUNT          347 a          242 c          197 g          353 t
ORIGIN      
        1 atttataaat cgtttttcag aaaaagtccc gtcttcgtta tataaataca taataaatac
       61 tgaatttggg atatagacag atttcttgga aagtgaaaac tctttcccat tttccagaaa
      121 aacatatcct ctttccatct tttcattttt ttcaaagcca attctgatat ttcttattct
      181 ttctgttcca tatatctgat gaacatcaaa atgtgtttca attctgggaa acatccgaat
      241 aggcagactg gctaccggcg aataccagta tctgtccacc tgcatcctga gacagccaga
      301 aaagaacaga atataaagaa atagagtttt cacattcatt agacttatac aaaagcagac
      361 tcaaagaaac agacgcattt ttttatggaa gaaagcgaag tcagccatat tttcaactga
      421 cttcctgagt attcctcagt ccacaaccca tcctgctgaa atcagagaaa ctacatctgt
      481 aaattccctt atgatttcac cggagtctct gcttctgatg atataggcat tccgcagggc
      541 gtcttccaga cggatgattc cgaactgatg ttcgctgctt ttcatacagc agtaaccggg
      601 aagaaaggca gaaacaggca gatcgtataa aacttctttt gtctggagtt tcagcctgtc
      661 cgaccaattc acccttttct tcctctcctt cactgcaaca gaactctttt tcattgtaat
      721 cctccctgcg ttttcgtaag tacaattata cagacgagcc ggacaaaatg gaagctgtat
      781 ctgttttttt tccgaaaatt tttcgactga aaatctcaga tgggtcatta ctgaattctg
      841 aagtaagcat cagggaggag aattttctga agagaaattc ggataaaaat aattgggatg
      901 attcagtcac cgtcacttga agatgcagtg cctgtaacat tgagtgctga agcaatatct
      961 gccgcaaaca gaagtctcag ttctttcaca tcactgtaaa gagtcttcac cgattggata
     1021 tccgaatcaa tgaactttcg taagctcagt cctgattcat tcattttact gatctgattt
     1081 tccgttttca gtatccgggc aagaacccta gcatccagaa caggattata atattttat
//
LOCUS       DAOQGJ010001749         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107166_length_1139_cov_2.505535, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001749 DAOQGJ010000000
VERSION     DAOQGJ010001749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107166_length_1139_cov_2.505535"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="sixA"
                     /locus_tag="PK683_21210"
     CDS             <1..523
                     /gene="sixA"
                     /locus_tag="PK683_21210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNH11030.1"
                     /translation="VKRATEEEVMKVVLVRHGEAEVSSESGKDIDRSLTDYGKNDIHR
                     LGMFMNRLFLKYNFIFHSPYLRTEQTAQILFEDLQKKQPMEIFPADELAPDNDYERIY
                     PKLKNFTNSDSVIIVGHNPNICYLGSRLISGNDVLAPNLNFLPGTALALNVSKEYLTS
                     AQILWMVSSKELL"
     gene            complement(474..935)
                     /locus_tag="PK683_21215"
     CDS             complement(474..935)
                     /locus_tag="PK683_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11031.1"
                     /translation="MLNFQKMNLFPYSILKYFIIILFLSVVLCSDRKKMPEFKGDYDS
                     YIKESALFMCAKIRECNPQIYRTFPEEYRDRKECETGLLNNWDEKIKNHREPEKQMLM
                     KDCISKVMKSDCKDFLYAGMRTHSCLDLYMRLQNESKEVLLKKPSKESEQK"
     gene            complement(914..>1139)
                     /locus_tag="PK683_21220"
     CDS             complement(914..>1139)
                     /locus_tag="PK683_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11032.1"
                     /translation="RYQAWYLKNFRESWAEKSFNYMENGMVYAYMPVLLSAAGASDSK
                     DSSANFTKALELLNSYYSRKGEIECSIFRK"
BASE COUNT          328 a          223 c          216 g          372 t
ORIGIN      
        1 cgtgaaaaga gccactgaag aagaggttat gaaagtagtt ttagtcagac atggggaagc
       61 tgaagtttct tcagaaagcg gaaaagacat tgacagaagc ttgacggatt atggtaagaa
      121 tgacatacac cgtctgggaa tgttcatgaa cagactgttt ctgaaataca attttatttt
      181 ccacagcccc tatctcagaa cagagcagac cgcacagatt ctctttgaag atctgcagaa
      241 aaaacagcct atggagatat tccccgctga tgagcttgct cctgataatg actatgaaag
      301 aatttatcct aaacttaaaa attttactaa ttcagattct gtcatcattg taggacataa
      361 tccgaatata tgttatctgg gatcaagact gatttcagga aatgatgtgc ttgctcccaa
      421 tctgaatttt cttccaggca ctgcgcttgc acttaatgta tcgaaagagt atcttacttc
      481 tgctcagatt ctttggatgg tttcttcaaa agaacttctt tagattcgtt ctgcagcctc
      541 atataaagat cgagacagga atgagtccgc attcctgcat acagaaaatc cttgcaatct
      601 gatttcatta ctttagatat gcagtccttc ataagcatct gtttttccgg ttctctgtga
      661 ttttttatct tttcatccca gttgttcagg agtcctgttt cgcactcttt cctgtctcgg
      721 tattcttccg ggaatgttct gtatatctgg ggattgcact ctcttatttt tgcacacata
      781 aacagagctg attcctttat ataggaatca taatctcctt tgaattcagg cattttttta
      841 cggtcggagc ataaaacgac tgacaggaat aaaataatta taaaatattt aagaatggag
      901 tatgggaaga gattcatttt ctgaaaattg agcattctat ttctcctttt cttgaatagt
      961 aggaattcag gagttcgagt gccttggtaa aatttgcact gctgtctttc gaatcagatg
     1021 ctccagctgc acttaagagg acaggcatat acgcataaac catcccgttt tccatataat
     1081 tgaaggattt ttctgcccag ctttccctga agttcttcag ataccaagcc tgatacctt
//
LOCUS       DAOQGJ010001750         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107245_length_1139_cov_1.600554, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001750 DAOQGJ010000000
VERSION     DAOQGJ010001750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107245_length_1139_cov_1.600554"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PK683_21225"
     CDS             <1..1006
                     /locus_tag="PK683_21225"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH11033.1"
                     /translation="KYGTEKIQNGSVLSPGKILSVGKRSYVDLQFIQLSPDAVVRINE
                     QSEFQFDKKIVSGKEENTFLLSLGRGLFDLKKLKQEDNIKVRTPVINAGVRGTKFIMN
                     ISKEKVSSVELLEGSLSVQPSIPELELIPEEIQKSSKALISVQENLKNSETVLEPGKK
                     IALGPKEISQYLSKSGLDKTLKLAEIISLKGKSSVSDEEKNKALALLDKTAADEKGKT
                     AIETKIPLKAEEIQNKDLQAKMKEFEDLLKLEKKKLEDSTDKTSIVSDFNKQREKQLR
                     KKIEQIFDLPTEVLILKNGTKVPGVIIEEGNKIIVLSPEGRKEFSRDDVDSRIELEEK
                     "
BASE COUNT          397 a          213 c          255 g          274 t
ORIGIN      
        1 gaaatacggg acggaaaaaa ttcagaacgg ttcagtctta agtcccggaa aaattctttc
       61 agtgggaaaa agatcatatg tagacttaca gtttatccag ctcagtcctg atgcagtggt
      121 gagaatcaat gaacagagtg aatttcagtt tgataagaaa atcgtaagcg gcaaggaaga
      181 aaatacattt ctgctttctt taggacgcgg gctgtttgat ctgaaaaagc tcaaacagga
      241 agacaatatc aaagtcagaa caccggtgat caatgcaggt gtccgcggta caaaattcat
      301 tatgaatatt tcaaaagaga aagtttcttc tgtggaactt ctggaaggaa gtctttctgt
      361 tcagccgtcc attcctgaac tcgaactgat acctgaagaa atccagaaat ccagcaaagc
      421 ccttatttct gttcaggaga atctgaaaaa ttcggaaact gttctggagc ctggaaaaaa
      481 aattgctctc ggtccaaaag aaatcagtca gtatctgtct aaatccggtc tggataaaac
      541 tctgaaactc gcagaaatca tctctttgaa agggaaatcc tcagtttcag acgaggaaaa
      601 aaacaaagca cttgcactgc tcgataaaac agcagcagac gagaagggaa agactgcgat
      661 agaaacaaaa attccgctga aggcggaaga gatacagaac aaggatctcc aggcaaaaat
      721 gaaagaattt gaagaccttc tgaagctgga aaagaaaaaa ctggaagact cgaccgataa
      781 aacatctatt gtcagtgatt tcaacaaaca gagagagaaa cagcttcgta aaaaaattga
      841 gcagattttt gatctgccca cagaagtcct gattctgaaa aacggtacaa aagttccggg
      901 tgtgatcatc gaagaaggaa ataaaatcat tgttctttca cctgagggta gaaaggaatt
      961 ttccagagac gatgtagatt ccagaatcga gcttgaggaa aaataatgaa ttccactgtt
     1021 gatttccggc agaccgttta cagtgagggg agtcgaacgg cggctttttc agtaaagacc
     1081 tccctccttc tttttctttg ttcctgtctg cctctgctgg cagggcagga aaagacagt
//
LOCUS       DAOQGJ010001751         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107317_length_1138_cov_2.963066, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001751 DAOQGJ010000000
VERSION     DAOQGJ010001751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107317_length_1138_cov_2.963066"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1138)
                     /locus_tag="PK683_21230"
     CDS             complement(<1..>1138)
                     /locus_tag="PK683_21230"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH11034.1"
                     /translation="AKKFLKTDTNFTGSETALNILTGGAVLSGTVNFFIPPNFALLSA
                     LAAAGFWSIVMFGVSLNALLKKYKPARIFFTAWLFLIAAVFISALNVAGVLNDNFFTL
                     HCMEIGSALEVILLSLALADRINFLKKENELAQKEVLNKQITLSKSFARFVPFEMLEF
                     LNRKIITEVELGDAVEKEMTVLFTDIRNYTSISEKFSPDENFRFLNFVLKDLGPIIRN
                     HSGVIDKFIGDAIMALFSGSPDNAVQAAVQMKRQLKILNRNLVQMNFPPIDIGIGINT
                     GKLILGTVGDEERMEATVISDTVNLASRIEGLTKHYHTEILLGEDSIRNLSETENYAF
                     RLIDRVLVKGKKKPINLFEVIDFSDAEKSKCLQYKDQMEKTIRLF"
BASE COUNT          328 a          230 c          231 g          349 t
ORIGIN      
        1 aaacagacgg attgtctttt ccatttggtc cttatattgt aagcattttg acttttcagc
       61 atcactgaaa tcaataacct caaacagatt gatcggtttt ttctttcctt tcactagaac
      121 tctgtctatg agccgaaatg cataattctc cgtttccgat aaatttctga tgctgtcttc
      181 tccaagaagg atttcagtat gataatgttt tgtcagtcct tcaattctgg aagccagatt
      241 tacagtatcc gaaatgacag tagcttccat tctttcttcg tcgccgacag ttcctagaat
      301 cagctttccg gtgttgattc caattccaat atcaataggg ggaaagttca tttgtaccag
      361 atttctgttc agtattttca gctgtctttt catttgaact gctgcctgga ctgcattgtc
      421 gggtgaaccg gaaaacaggg ccatgattgc atcaccgata aatttgtcga tgactccgct
      481 gtgatttcgg atgatcggac ctaagtcttt gagcacaaaa ttcagaaatc gaaaattctc
      541 atccgggctg aatttttcag aaatggaagt ataatttcga atatccgtaa agagaacggt
      601 catttctttt tcgacagcat cgccgagttc aacctcggta atgatttttc gatttaaaaa
      661 ttcaagcatt tcgaaaggca caaagcgggc aaaggatttg gaaagagtga tctgtttatt
      721 caagacttcc ttctgtgcca gctcgttctc tttcttcagg aaattgattc tgtcagcgag
      781 agccagggaa agaagaatga cttccagtgc agagccgatt tccatacagt gtaatgtaaa
      841 aaaattatcg tttaatacac cggctacatt cagtgcggag atgaaaacag cagcaatcag
      901 aaacagccag gccgtaaaaa atattctggc aggtttgtat tttttcaaga gagcattcag
      961 acttactccg aacattacga ttgaccagaa tcctgctgca gcaagtgcag agagaagagc
     1021 aaaattcgga ggaatgaaaa aatttaccgt tccggataaa acggctccgc cggtcaatat
     1081 gttcagcgca gtttcggagc ctgtaaaatt cgtatcagtt ttcaggaatt tttttgca
//
LOCUS       DAOQGJ010001752         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107336_length_1138_cov_2.650970, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001752 DAOQGJ010000000
VERSION     DAOQGJ010001752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107336_length_1138_cov_2.650970"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PK683_21235"
     CDS             <1..836
                     /locus_tag="PK683_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH11035.1"
                     /translation="GRERYSINIRYPRELRDSPDRLKKVLVPTEKGHVPLGQIAKIEF
                     KSGPSMIRNENGLLAGYVFVDTKETDLGGYADRARRLISEKVKLPAGYNLLWSGQYES
                     LERMKEKMKIIVPFTLLLIGFLLRMSTGSWQKTMIILLAVPFSLVGAVSLLFILQYEI
                     STAVWVGMIALLGLDAETGAFMLLYLDLEYEKLMKTTDTPSISQLQEAILHGSVRRVR
                     PKIMTVMCGIAGLLPILWSSGAGSDLMKRIAAPMVGGLVTSFILELLVYPAVYLLWRR
                     R"
     gene            complement(855..>1138)
                     /locus_tag="PK683_21240"
     CDS             complement(855..>1138)
                     /locus_tag="PK683_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11036.1"
                     /translation="EARRKAELDYFSGMTEAFEKGIEKGIEKGKTEGIEEGLYLGIQL
                     NLESRFNSKGNALLKKIRKLKDLNLLKKILVLSAKAESIQEIRYFLNKI"
BASE COUNT          287 a          237 c          267 g          347 t
ORIGIN      
        1 aagggagaga aagatattct ataaatataa gatatcccag agaactgaga gacagtccag
       61 accgtctgaa gaaggttctt gtgcctacgg aaaagggtca tgtgcctctc ggacagattg
      121 caaagattga attcaagtct ggaccttcca tgatccggaa tgaaaacggg cttctggcag
      181 gatatgtttt tgtggacaca aaagagacgg atctgggagg ttatgcggac cgtgcaagaa
      241 gactgatttc tgaaaaagtg aaacttcctg caggatacaa tcttctctgg agcggtcagt
      301 atgaaagtct cgaaaggatg aaagagaaga tgaaaatcat tgtgcctttc actcttcttc
      361 tcatcggatt tctgcttcgg atgagcacag gttcctggca gaagacaatg atcattctgc
      421 ttgcagttcc tttctctctt gtgggtgcag tgagtctgct ttttattctt cagtatgaga
      481 tctccactgc tgtctgggtg gggatgattg cacttcttgg actggatgca gagaccggag
      541 ctttcatgct tctgtatctc gatctggaat atgagaagct gatgaagacc accgataccc
      601 cttccatttc acagcttcag gaagctatcc ttcacggttc agtcagaaga gtgcgcccaa
      661 agatcatgac agtgatgtgc ggaattgcag gtcttctccc gatcctctgg tcaagcggag
      721 caggatctga tttaatgaaa agaattgcag cccctatggt cggaggtctt gtgaccagtt
      781 tcatcctgga gcttcttgta taccctgcag tgtatctgct ctggagaagg aggtgaggag
      841 tattttataa taaattatat tttatttaag aaatacctga tttcctgaat actttcggct
      901 ttcgcactta agaccagaat ttttttcaga agattcaagt ctttgagttt tctgattttt
      961 ttcagaagtg cattgccttt ggaattgaac cgggattcca gattcagctg tatccccaga
     1021 tacagccctt cttcaatccc ttctgtcttt cctttctcga ttcctttttc gattcctttt
     1081 tcaaaggctt cggtcattcc ggaaaaataa tcgagctctg ctttccggcg ggcttcgt
//
LOCUS       DAOQGJ010001753         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107497_length_1137_cov_3.573013, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001753 DAOQGJ010000000
VERSION     DAOQGJ010001753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107497_length_1137_cov_3.573013"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PK683_21245"
     CDS             complement(<1..491)
                     /locus_tag="PK683_21245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11037.1"
                     /translation="MNKKKNSSSKKKNIPPLLTTTKIQIEDTDDSIEALEETKESSVS
                     SGPAKPPMSTFTKGLILILVSSLVISFQNIVTRVILSKKMVLGIWEIGGFVKPSMANS
                     ILILILRMAIVVPIMAFLVAPRIYNNTWIDIRGLFKAENKNKKFIVIGSGVLLFMSFF
                     CMYL"
     gene            895..>1137
                     /locus_tag="PK683_21250"
     CDS             895..>1137
                     /locus_tag="PK683_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11038.1"
                     /translation="MLSKANIQFFRTALSEEAIKGEIQRLRFLSGIFLPAALLLAILP
                     QILAKEFSIIYKGSNLGYAIPGVMILFSLYGFICRKI"
BASE COUNT          353 a          224 c          206 g          354 t
ORIGIN      
        1 agatacatac agaagaatga cataaacaga agaacaccgc ttccaatcac aataaatttt
       61 ttgtttttgt tttctgcttt gaagagtccc cgtatatcaa tccaggtatt gttgtatatt
      121 ctgggtgcaa caagaaaggc cattatgggt acaacgatag ccattctcag tatgagtatg
      181 agtatggaat tcgccatact cggcttgaca aaaccgccaa tctcccaaat cccgagaacc
      241 atcttcttgc tcaggattac cctagtgaca atattctgaa acgaaataac cagagaagaa
      301 accagaatca ggataagacc ctttgtaaaa gtggacattg gaggctttgc tgggcctgaa
      361 gaaacagagg actcctttgt ctcttccagt gcttctattg aatcgtctgt atcttcaatc
      421 tgaatcttgg ttgtagtgag taaaggcggg atgttctttt tcttgctgga gctgtttttt
      481 ttcttgttca tttactgtaa tccctctgcc ctcaaatttc ttgtcattgt atactgactc
      541 gtttttaata aaaggaaaaa acgagagtaa tatacaatta aatccaacaa ttgcattcca
      601 tttttttatc taagctctca aaaattttta caatttgaga aactttatga tccaatcttt
      661 ctgcttccgg ataaaagcat ctgaattgag catatgcaga cgcagacttg cggctggcct
      721 tactcataga aacggatcaa acctgaaaaa tttaaagtaa aatattccta aaaactgaaa
      781 tgatcgaaag tttctcacag tacaagccgt cataaatttt tcttgcatca taggattgca
      841 taattattgt aatcgaggtt caaagaacag agattttctt ccgaaggaat tgtaatgcta
      901 tcgaaagcca atattcaatt tttcagaact gcactttcag aagaagcaat caaaggagaa
      961 attcagagac tgaggttcct ttcgggaata tttcttcctg cagccttgct gctggcgata
     1021 cttcctcaaa tactggcaaa agaattctct atcatctata aagggtcaaa cctgggttat
     1081 gcaatcccgg gagtcatgat tcttttttcc ttgtacggct tcatctgccg aaagata
//
LOCUS       DAOQGJ010001754         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107524_length_1137_cov_2.869686, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001754 DAOQGJ010000000
VERSION     DAOQGJ010001754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107524_length_1137_cov_2.869686"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PK683_21255"
     CDS             complement(<1..548)
                     /locus_tag="PK683_21255"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNH11039.1"
                     /translation="MSGLAYELVAGTMATYLLGQSVTQFSYATGWFLASMGFGSYLSR
                     YIKVRLLETLLRVQVILGIVGGYSAFLMFLSFAFTETVYPVFMVLAFVIGTCVGLEIP
                     ILLRMIGKYRILSIAVSDVFTYDYIGALFASLLFPLLLLPYLGILRTSLFFGLMNLAA
                     AWILLSLMDKKRRFYRILLILAS"
     gene            complement(605..835)
                     /locus_tag="PK683_21260"
     CDS             complement(605..835)
                     /locus_tag="PK683_21260"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNH11040.1"
                     /translation="MPDWLVHENLKGAVIVETLLYTAIGLMVFALAIWIMNKVVPFSL
                     RKEIEEDQNTSLGIIIGSVIIGIALIISAILK"
     gene            complement(871..1083)
                     /locus_tag="PK683_21265"
     CDS             complement(871..1083)
                     /locus_tag="PK683_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11041.1"
                     /translation="MMIRFIQITAVILLIVSAYYTYACATNAIIEYAEEGKISLKEES
                     VRTGRTTFFYDTRRRSPSGGGWGFGK"
BASE COUNT          381 a          276 c          211 g          269 t
ORIGIN      
        1 gacgcaagga tcagaaggat tctgtagaac cgtctttttt tatccatcaa agaaagaaga
       61 atccatgctg ccgcaagatt catcagtcca aagaatagac ttgttctgag aattcccaga
      121 tagggaagaa gaagcagagg aaaaagcaga ctcgcaaaga gcgcaccgat ataatcgtac
      181 gtaaatacat ccgagactgc aatggaaagt attctgtatt ttccgatcat acggagaaga
      241 ataggaattt ccagacctac acaggtgccg attacaaatg caagcaccat aaatacaggg
      301 taaaccgttt ctgtgaatgc gaaactcaga aacatcagaa aggcagaata ccctcccaca
      361 atgcccagaa tcacctgcac tctcagaaga gtttccaaaa gacgcacttt tatataacgg
      421 gacagatagg aaccgaatcc cattgatgca agaaaccagc ctgtagcgta cgaaaactga
      481 gtaactgact ggcctaaaag ataggtagcc atcgtgcctg cgaccagctc atacgccagc
      541 ccagacactg aaatgatcag aattgtcagg agaaggagaa acctcttcct ttccccacgg
      601 tcaatcactt gagaatagcg gagataatca gagcaatacc gataatcacg gagccgataa
      661 tgatacccaa agaagtgttc tgatcttctt caatttcctt tctcaaagaa aacggcacaa
      721 ccttgttcat aatccagata gccaaagcaa aaaccatcag tccgatcgca gtgtaaagca
      781 gtgtctctac aataactgca ccttttaaat tttcatgtac caaccaatcc ggcataataa
      841 cccccaatct tccttattat attaaaattt ctatttcccg aatccccatc cgccgccgga
      901 cggagaacgt ctgcgagtgt catagaaaaa tgtagttctt cctgtcctga cactctcttc
      961 tttcaggctg attttgcctt cttcagcata ttcaatgatt gcatttgtag cacatgcata
     1021 agtataataa gcagacacaa tcagcagaat cactgcagta atttggataa atctaatcat
     1081 catcggcctc cggcattacg tccgaccatc tggaactttc aaaaaaccat cgtctca
//
LOCUS       DAOQGJ010001755         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107537_length_1137_cov_2.680222, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001755 DAOQGJ010000000
VERSION     DAOQGJ010001755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107537_length_1137_cov_2.680222"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..>1137)
                     /locus_tag="PK683_21270"
     CDS             complement(145..>1137)
                     /locus_tag="PK683_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11042.1"
                     /translation="LLIIPVLGLGAGIVGIHYYLGQTKFERTPLFSEKNPEGFSTSTR
                     KGSFLNLKNTRFRFKENISLDITRLTAELIPKGNSSIVNFDDVNSFLIKIANGEAYAD
                     ASVLEVLFKDYVFKFEGSPLKLDKIEMLEGNDNRIRMTGEMNLFIWLNFEMIGTLSLD
                     KASNNVVITAEDIRAVVPTNSKPILGMIGLNLEKLLTIPEGRGIAVKKNQIFVSVFSI
                     IPPPQMEGTIGEIAIKNKRMYLKLESPFEPKFPPPPEPNVKNYLHVFRGEVKFGKLFM
                     VDANMQMIDKDQNDPMDFYLEKYFVTLTSGGSAVINPDQSVKVYMPDYSDVTGR"
BASE COUNT          313 a          255 c          228 g          341 t
ORIGIN      
        1 ctcctgatca gctcctcttt tgaaagagaa gatccgagca gggcaatgat cgaggcaagt
       61 cctgcaaatg gaagagcaaa cagtgtatgc gaaaacttga ccattcggcc aaaatcagtg
      121 aaaatctcca gcaggcgcat tttttcagcg tcctgttacg tcactgtaat ccggcatgta
      181 cacttttaca gactgatccg gattgatgac agcagatccg ccgctggtca gtgtgacaaa
      241 gtatttttcc agataaaaat ccatcggatc attctgatcc ttgtcgatca tctgcatatt
      301 tgcatctacc atgaaaagtt ttccaaactt cacctcaccc cgaaaaacgt gaagatagtt
      361 tttgacattc ggttccggag gagggggaaa ctttggctcg aaaggagatt caagtttcag
      421 atacattctt ttatttttta ttgcaatctc tccgatcgtt ccttccatct gagggggcgg
      481 aatgattgaa aatacactta caaaaatctg atttttcttc actgcaatcc ctctgccttc
      541 cggaatcgtc agcagttttt cgaggttcag tccgatcatt cccagaatag gctttgagtt
      601 tgtgggaact actgcacgga tgtcttctgc agtaatgacc acattattgg aagccttgtc
      661 cagcgaaaga gttcctatca tttcaaaatt cagccatatg aagagattca tttcccctgt
      721 cattctgatc ctgttgtcat ttccttcgag catctctatt ttatccagtt tcaacggaga
      781 gccttcaaac ttgaatacat agtccttgaa gagcacttcc agaacggatg catctgcata
      841 ggcctcaccg tttgctatct tgatgagaaa tgaattcacg tcatcaaagt ttacaatgga
      901 agaatttcct ttaggaataa gttctgcagt cagcctggta atatccagac tgatattttc
      961 cttgaatctg aatctggtat ttttcagatt caggaaggag ccttttctgg tgcttgtgga
     1021 aaaaccttcc ggattcttct ccgaaaaaag aggagtcctt tcaaactttg tctgacccaa
     1081 ataataatgg attcctacga tgcctgcacc taggcctaga actgggataa tcaaaag
//
LOCUS       DAOQGJ010001756         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107839_length_1135_cov_3.322222, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001756 DAOQGJ010000000
VERSION     DAOQGJ010001756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107839_length_1135_cov_3.322222"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PK683_21275"
     CDS             complement(<1..255)
                     /locus_tag="PK683_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004473776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH11043.1"
                     /translation="MMFNQTETEDLIERILNGDGKALEDLLKSHQDYIYNLCRSMFLN
                     PMDAEDTTQEILIKTVSSLSKYDKTKARFSTWLYRITVNHT"
     gene            complement(236..622)
                     /locus_tag="PK683_21280"
     CDS             complement(236..622)
                     /locus_tag="PK683_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11044.1"
                     /translation="MSFLKKGKFINAATAGLVPAALELLLIVSVEPSIDRWVLFQAVF
                     FWFTCGFSVYRLCSEERIISSSVGYTVFLNIPWYTAESAGKNKPEHLVPLVCVSILQG
                     IIIGILAKKLKQSKIGREIKYDVQSD"
     gene            complement(622..972)
                     /locus_tag="PK683_21285"
     CDS             complement(622..972)
                     /locus_tag="PK683_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11045.1"
                     /translation="MNKTNNGSPVIEWASFELAENTTEAQLLAASDDLQRNFLDKQPG
                     FLKRELLFLSDRKWVDLVHWENGDCSEKAMDSAMQSEACLKYFHLMVPADQQSGAGVI
                     HLTQKKAYFSGKRL"
BASE COUNT          303 a          260 c          215 g          357 t
ORIGIN      
        1 agtatgattc actgtaattc tgtaaagcca agtgctgaac cttgcctttg tcttgtcgta
       61 ctttgaaaga ctggaaactg ttttaatcag tatttcctgt gttgtgtctt ccgcatccat
      121 aggattcaga aacattgatc tgcatagatt gtaaatatag tcctgatggg atttgagaag
      181 gtcttccagt gctttcccgt ctccgttcag aatcctttca ataaggtctt cggtttcagt
      241 ctgattgaac atcatatttt atctccctgc caattttgga ctgtttcagc tttttggcca
      301 atattccaat tatgatcccc tgcagaatgc ttacacatac aagagggaca agatgctccg
      361 gcttgttttt tcctgctgat tcagcagtgt accatgggat atttaagaat acagtatatc
      421 ctaccgaaga agaaataatt ctctcttctg aacagagacg atatactgag aacccgcagg
      481 tgaaccagaa aaatacagct tgaaaaagaa cccatctgtc aatagacggc tccaccgaga
      541 caatcagcag cagttccaga gcagcaggaa caagtcctgc tgtggccgca tttatgaatt
      601 ttcccttttt caaaaaactc atcacagccg ttttcctgaa aagtatgctt ttttttgagt
      661 cagatggata acacctgctc ctgactgctg gtctgcagga accatcaagt ggaaatattt
      721 cagacaggct tcactctgca tagcactgtc cattgctttt tcggaacagt ctccgttttc
      781 ccaatgtaca agatcaaccc atttccggtc actcaggaat aatagctccc ttttcaggaa
      841 ccctggctgt ttatccaaaa agtttctctg cagatcatct gatgcagcta gaagctgcgc
      901 ctctgttgta ttctctgcca gctcaaaaga tgcccattca ataactggac tgccattgtt
      961 tgttttattc atacattatc tccttctacg gtctgcttca accgctgtca taaagtgata
     1021 ctttcagaat ggagatctgt gacagtgctg ctggaaaaat ttcagttttt taaaaaatct
     1081 ctcatcggct taggcgggta tcccagtttt gagaaaattt ccagaactct gcttc
//
LOCUS       DAOQGJ010001757         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107843_length_1135_cov_3.234259, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001757 DAOQGJ010000000
VERSION     DAOQGJ010001757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107843_length_1135_cov_3.234259"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK683_21290"
     CDS             complement(<1..355)
                     /locus_tag="PK683_21290"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH11046.1"
                     /translation="MSSANITEIGSAEFETKILNEKSPAVLDFYSDECAPCESLAPKY
                     EEMADLFGENVRFYKIFRQQNRELANSLGVSSSPTVIFFKDGKETAERLMGGIKKREL
                     LENIRIKPYSPSVRRG"
     gene            complement(662..>1135)
                     /locus_tag="PK683_21295"
     CDS             complement(662..>1135)
                     /locus_tag="PK683_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11047.1"
                     /translation="IEQCISNPQLLDKGKSPDCGSEIGTASFIDNDHILIWAFRDEEA
                     EDDETEKLPAGHFAVWNFKTDEISKPLKPESKIGNLAAINSEYAWDFYEYPKIISLKT
                     GKTEYEARDINSGMQSSSIMHHLPEPLPLISVEKGNRRAAFRNGSSVEVLECDMD"
BASE COUNT          308 a          260 c          219 g          348 t
ORIGIN      
        1 ggccccgtct caccgatggg gaatagggtt ttatgcggat attttccaga agttctctct
       61 ttttaattcc gcccatcagt ctttctgcag tctccttccc gtctttgaaa aaaatgactg
      121 taggactgga tgaaactccg agagaatttg ccagttccct gttctgctga cggaatattt
      181 tgtaaaaacg cacattttcc ccgaaaagat cggccatttc ctcatatttc ggagcaagtg
      241 attcgcaggg agcacactca tcggaataaa aatccagaac agcaggactt ttttcgttca
      301 gtatttttgt ctcaaattca gccgatccga tttcagttat atttgcggaa ctcattactc
      361 tctcctatat accctacacg gtatagtata taggcttttt cgtcaagaac taaactgctc
      421 cattttgaga aaaaatacat cgccaagaga atatcttcct gctggctaaa gaacattgac
      481 acacggactc tttgggagtg cagaggaatc agagatcatc tgtagaaggt attgattctg
      541 acgctgccgt ttcaaaaatc attgaatact gccttggaaa ttagggaata gagacggaat
      601 ctactttgca gaagggtatt cgccggagct tcttctgtca ggaatctatt tcagtatgta
      661 attaatccat atcacattca agaacttcca cggaactgcc gtttctgaat gctgcccttc
      721 tatttccttt ctcaacagaa atcagaggaa gaggttcagg cagatggtgc ataatcgacg
      781 aactctgcat tcccgaattt atatcccttg cctcatattc agtctttcca gttttgagac
      841 tgatgatctt gggatactca taaaaatccc aggcatattc tgaattgatc gcagccagat
      901 ttccgatttt tgattccggc ttcaacggtt tggaaatttc atccgtcttg aaattccaca
      961 cagcaaaatg accggcagga agcttttcag tctcgtcatc ttctgcttct tcatctctga
     1021 acgcccatat cagaatatga tcattgtcaa taaaacttgc cgtgccaatt tctgaaccgc
     1081 agtcaggaga ttttccctta tccaaaagct gcggattgga tatgcactgc tctat
//
LOCUS       DAOQGJ010001758         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_107852_length_1135_cov_3.111111, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001758 DAOQGJ010000000
VERSION     DAOQGJ010001758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107852_length_1135_cov_3.111111"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK683_21300"
     CDS             complement(<1..355)
                     /locus_tag="PK683_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11048.1"
                     /translation="MKIRLNLISFALIIVSVVFCDKKKDDKTKSLSLLALAALSNTTA
                     AQYPNSVSGSTEAVKGAQGASSGVSAATSAATSGAQSVAQSVLGSERANIIAKGLAAS
                     KAMNTCDNTERTVTGT"
     gene            624..992
                     /locus_tag="PK683_21305"
     CDS             624..992
                     /locus_tag="PK683_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNH11049.1"
                     /translation="MKDTKETKHRIYTTSFASVYPHYIAKAEKKGRTKAEVDEIILWH
                     TGYSQKELEDHLKNKTDFETFFADAPLLNPSRKLIKGVVCGVRVEEIKEPTMQEIRYL
                     DKMIDELAKGKKMEKIFRKQ"
BASE COUNT          364 a          216 c          229 g          326 t
ORIGIN      
        1 aagttcctgt cactgtcctt tcagtattgt cgcaggtgtt cattgccttt gatgcagcaa
       61 gtccttttgc gataatgttc gctctttcgg aaccaagcac actctgtgca acactttgtg
      121 ctcctgaagt agctgctgaa gtggctgctg aaaccccgga agaagctccc tgggcacctt
      181 tcacagcttc ggtggatccg ctgactgaat ttggatactg tgctgctgta gtattggaaa
      241 gtgccgctag agccagaagt gacagacttt ttgttttgtc gtcttttttc ttatcacaga
      301 agacaactga tacaatgatt aacgcaaaag aaatgagatt taatcttatt ttcataaaat
      361 tcccctaatt ttttgtcgcc gttaaaagcg caggactcat atttcatatt tatacttttt
      421 tgtcaatatt aaaaaaatat cagctttata atggttctaa accacatttg agtgtatttt
      481 attcgtgact gcccagcctg aaggcttcat cgttacaaac agaaagcatt ctgcagttta
      541 tcctgaaagt tccagaacaa tttcagtcct ttatcaagaa tttatttgaa gacaggaatc
      601 ataatgaaaa aaagtctaat gaaatgaaag atacaaaaga aacaaaacac cgcatctaca
      661 ccacaagttt tgcaagcgtg tatccgcact acattgccaa agcagaaaaa aagggacgca
      721 caaaggcaga agtggatgaa atcatcctct ggcatacagg gtacagtcag aaagaattag
      781 aagatcatct taaaaataaa acagactttg agacattttt tgcagatgct cccctgctga
      841 atccgtccag aaaactgata aaaggtgttg tatgcggggt cagagtagaa gaaataaaag
      901 agccgactat gcaggaaata cgttatctgg ataagatgat tgatgaactg gctaaaggaa
      961 agaaaatgga gaaaattttc cgaaaacagt gactgaatcg ataatcggaa aaatacttta
     1021 gaataatttc gtcagcggag caattccgcc gacgtaaact atgaatccgt taatgtgagg
     1081 cttttttatc gtggtatttg ctgagctgat gctccatttt atcttcgaga ccgtc
//
LOCUS       DAOQGJ010001759         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108042_length_1134_cov_3.229842, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001759 DAOQGJ010000000
VERSION     DAOQGJ010001759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108042_length_1134_cov_3.229842"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PK683_21310"
     CDS             <1..265
                     /locus_tag="PK683_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11050.1"
                     /translation="AVKKREIWEENLDGVDAAKALKKKTDKNLPRPRICPVCGKALKK
                     HEYLFASMEEARGQEKRQVKIYGCRYCYLGLSDDPAIREGLEL"
     gene            265..>1134
                     /locus_tag="PK683_21315"
     CDS             265..>1134
                     /locus_tag="PK683_21315"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNH11051.1"
                     /translation="MKNISLDSDLSILSGVGPKKQKVLENFGLKKISDLLYFFPRKYL
                     DRNFTGDFFLKAGETVTFLAEIKSFYTAHGKKSRLIAVCTTNRGEQIELVFFRGLEFF
                     RKIIYKGLQLAVTGKLENFRGTFQIVHPEYETVTEDDPSMLKHTGRIIPLYPSNETLK
                     NEGLDSKGFRRLQIQIQDWIDEGRISVPEIIPPELAKKRNLKSRKDAFLEIHFPESFE
                     SLKDAQFRFAYEELYFFSILMEYKKNLRNEFKRKLWPLPDSKTASELIKTLPFSLTED
                     QENAVSLLRKSSSG"
BASE COUNT          367 a          213 c          256 g          298 t
ORIGIN      
        1 ggctgtgaaa aagagggaga tctgggaaga aaatctggac ggagtggatg ctgcaaaagc
       61 tctgaaaaag aaaacggaca aaaatctgcc tagaccgaga atctgccctg tctgcggaaa
      121 agcattaaaa aagcatgaat atctgtttgc atccatggaa gaggcaagag ggcaggaaaa
      181 aagacaggtg aagatatacg gatgcagata ctgttatctt ggactttccg atgatcctgc
      241 gatcagagaa gggcttgaac tctgatgaaa aacatttcac ttgactccga tctttccata
      301 ctgtctggag tcggtccgaa gaaacagaaa gtactcgaaa atttcggtct gaagaagatt
      361 tcagaccttc tctatttctt tcccagaaaa tatctggaca gaaatttcac aggggatttt
      421 tttctgaagg cgggagagac ggtcacgttt ctcgctgaaa taaaatcctt ttacactgct
      481 cacggaaaaa agtccagact gattgcggtc tgcacgacaa acaggggcga acagattgaa
      541 cttgtatttt tcagaggtct ggaatttttt cgtaaaatta tttataaagg tctgcagctc
      601 gctgttacag ggaaactgga aaatttcaga ggtacattcc agattgttca ccctgagtac
      661 gaaacagtca ctgaagatga tccttccatg ctgaaacata caggaaggat tattcctctt
      721 tatccttcaa atgaaacttt gaaaaatgaa ggtcttgatt ccaaaggatt caggagactc
      781 cagatccaga tccaggactg gatagacgag ggcaggattt ctgttcctga aattattccg
      841 cctgaactgg caaaaaaaag aaatctgaaa tcgagaaagg atgcatttct tgaaattcat
      901 tttccggaaa gttttgaatc gctgaaggat gcacagttcc gctttgctta tgaggaactg
      961 tatttttttt ccattctgat ggaatacaaa aagaatctca ggaatgaatt caagagaaaa
     1021 ctctggccgc ttcctgattc aaagactgca tctgaactga taaagactct tcctttctct
     1081 ctgacagaag atcaggaaaa tgcagtcagt cttctcagga aaagttcatc aggg
//
LOCUS       DAOQGJ010001760         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108046_length_1134_cov_3.178869, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001760 DAOQGJ010000000
VERSION     DAOQGJ010001760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108046_length_1134_cov_3.178869"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PK683_21320"
     CDS             <1..865
                     /locus_tag="PK683_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11052.1"
                     /translation="KLTGTAKEKIGFYPLPSDQPERIWLQLLSLQRKTSFKNRTFIIS
                     VSPITLSKNEVTDAHKSLFHNYHPFLPSIMTEPDIMKFYFKDGAGLVYYLFGRLFPVL
                     RLNGFYSTEIKFLKKEISPENKDFRSALNEGFFSALKKNGEMNHHLEETLLKNDFHWE
                     WGTAKEETECHFRADRNSFVKSSVQILYRERPDSVKTWNKIFDFLQKEEARFLFVRIP
                     FSPMLEDALSEAEAPDVKLFKEWEQKKKGSVFTVDSDDFSIGDFTDLTHLNSCGRKKF
                     QKALKDKIKGF"
     gene            complement(898..1074)
                     /locus_tag="PK683_21325"
     CDS             complement(898..1074)
                     /locus_tag="PK683_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNH11053.1"
                     /translation="MDTYDTVVKIFKTSKEPLSAGDIVKLIGLDRKEVDKVLNKLKKE
                     EKIISPKRCYWSLK"
BASE COUNT          347 a          231 c          213 g          343 t
ORIGIN      
        1 gaaactgact ggcactgcaa aggaaaaaat cggattctat cctctgccct cggaccagcc
       61 ggaaaggatc tggcttcagc ttctgtctct tcaaagaaaa acgtccttca aaaacagaac
      121 gtttatcatc agtgtatctc cgatcacact gtccaaaaat gaagtgactg atgcgcataa
      181 atcattattt cataattatc atccttttct gccttccatc atgacggaac cggatataat
      241 gaaattttat ttcaaagacg gtgcaggtct tgtttattat ctgtttggaa gactttttcc
      301 cgttctccgg ttgaacggtt tttacagcac cgaaatcaag tttctgaaaa aggagatttc
      361 tcccgaaaat aaggattttc ggtctgcact caatgagggc ttcttttctg ctctcaaaaa
      421 aaacggtgag atgaatcatc atctggaaga aactcttctg aagaatgatt ttcactggga
      481 atggggaacc gcaaaagaag aaacggaatg ccatttcaga gcagacagaa attcctttgt
      541 gaaaagctcg gtacagattc tgtacagaga acggccggac tcagtaaaaa cctggaataa
      601 aatttttgac ttcctgcaga aagaagaagc gagatttctt tttgtcagaa ttcctttttc
      661 accgatgctt gaagacgctc tttcagaggc agaagcccct gatgtaaaac ttttcaaaga
      721 atgggaacag aaaaaaaaag gatctgtttt cacagtggat tcagatgatt tttcgatcgg
      781 cgactttacc gatctcaccc atctgaattc ctgcggaaga aaaaaatttc agaaggcttt
      841 gaaagataag attaaaggtt tttgaattta atccccttta cagctgagac taattctcta
      901 tttcaaagac cagtaacatc gtttaggaga gatgatcttt tcttccttct tcagtttatt
      961 cagaaccttg tccacttcct ttctgtcaag gccgatcagc ttgacaatat ctccggcact
     1021 caaaggttcc tttgaagttt tgaaaatttt aactactgta tcataagtat ccatatgtat
     1081 tctcctgact gttaactcag aatgaatatc tcagtatagc aagctttttt ccgt
//
LOCUS       DAOQGJ010001761         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108211_length_1133_cov_3.756030, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001761 DAOQGJ010000000
VERSION     DAOQGJ010001761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108211_length_1133_cov_3.756030"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK683_21330"
     CDS             <1..192
                     /locus_tag="PK683_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11054.1"
                     /translation="LPMKDYFPFEMEDALNAFYSVFADRKKEDIISRFPDLKIFHIQD
                     TLNTVLYRRAGKKERRERT"
     gene            217..>1133
                     /locus_tag="PK683_21335"
     CDS             217..>1133
                     /locus_tag="PK683_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH11055.1"
                     /translation="MSQDKSYKKVSESPTIPGHSKKEKKFDVMNLYSGPASAEGMNSD
                     ESGTENLDEKLRKAYFWIANHAIISPYYDIEYNNGKFHSFTVGDFKTSVNFPSGQSYS
                     SYVLLPLLTLVTRRKCLLVGGPGRGKTASAVLMGVLAGYTITEIKRGMQHGQPQMTVS
                     DLLGNPLPSDLMNAKSMDDIKIAWRKWLDMRVKIIDEYNRIPTRTQSALLTVLADNYA
                     EMFDQVKQCPDAAWYLTANDDAGGGTYDVIEALKDRIDIVVKALHFNTRFLRELLTRI
                     EENFKPEEIVPSEIIFTEEEISRMNKAILA"
BASE COUNT          356 a          229 c          262 g          286 t
ORIGIN      
        1 ctgcctatga aagactattt tccctttgaa atggaagatg cactgaatgc attctactct
       61 gtctttgctg accggaaaaa ggaagacatc atttccagat tccccgatct gaagatattt
      121 catatacagg atactctgaa tacagttctc tacagacggg cagggaaaaa agaacgcagg
      181 gaaagaacat aattttacat taaaaaagga tataaaatga gtcaggataa gagctacaag
      241 aaagtatcgg aatcgcctac tattccggga cattcaaaga aggaaaaaaa attcgatgta
      301 atgaatcttt attccgggcc cgcctcagcg gagggcatga attcagacga gtcaggcact
      361 gagaatctgg atgaaaagct gagaaaggct tatttctgga ttgcaaatca tgcgattatc
      421 agtccttatt acgatataga atacaataac ggaaagtttc acagcttcac cgtgggcgac
      481 ttcaagactt cagtcaattt tccttcgggg cagagttatt ccagttatgt tcttcttcct
      541 cttctcactc ttgtcaccag aagaaaatgt cttctggtag gaggtcccgg ccgaggcaag
      601 acagccagtg cagttctgat gggagttctg gcaggataca caatcactga aatcaagcgg
      661 ggaatgcagc acggacagcc tcagatgact gtttctgatc ttctgggcaa tcctcttccc
      721 tccgatctga tgaatgcaaa gagcatggac gacatcaaaa tagcctggag aaaatggctg
      781 gatatgaggg tgaagatcat tgatgaatac aaccgtatcc cgacaagaac tcagtctgcg
      841 cttctgactg tgcttgcaga caattatgca gaaatgttcg atcaggtaaa gcagtgtcct
      901 gatgctgcct ggtatctgac tgcaaatgac gatgcgggag ggggaaccta tgatgtcatc
      961 gaagctctga aggacaggat tgacattgta gtcaaggctc tccatttcaa cacaagattt
     1021 ttaagagagc ttctgacaag gatcgaggaa aatttcaagc cggaagaaat tgtaccctct
     1081 gaaattattt ttactgaaga agaaatttcc agaatgaaca aggcaatcct tgc
//
LOCUS       DAOQGJ010001762         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108237_length_1133_cov_3.084416, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001762 DAOQGJ010000000
VERSION     DAOQGJ010001762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108237_length_1133_cov_3.084416"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PK683_21340"
     CDS             complement(<1..583)
                     /locus_tag="PK683_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11056.1"
                     /translation="MKYILYCFLILFLQCSILYDKRTGDLSIKLQGWRAENKNPVLLQ
                     KDEKLTGKLRFLFEDINGVQEKDDYPLMIFSETFPELQFSESFAVGFFTAGSWYSTFP
                     YMWTYPSDKSIHLKSREIHLSRNGNEVPYGEYFAYISNAEIEEGGFIKNNSYLVTFGY
                     EYDYDTYTFKEYNGECKHSSVPNIHWLGKLYNPR"
     gene            complement(619..>1133)
                     /locus_tag="PK683_21345"
     CDS             complement(619..>1133)
                     /locus_tag="PK683_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11057.1"
                     /translation="SQTDHEYGMGKLSALAGGYRAVYQAKKGSVNAGWHILGSPFYLG
                     IGLGVENNFQQGWFGYFNNEIVSGPGVWRGMNKYREYRLWIDNSAFRNLDFGLRWVFP
                     AGIITGLQCGQMHFFNRTMNAKIDMLAYEGRNANGLDLLNEIRESKLQYQGRTGLSEV
                     NWMFYVGYAF"
BASE COUNT          339 a          273 c          174 g          347 t
ORIGIN      
        1 cccttggatt atacagtttc cccagccaat gaatattcgg cacactggag tgtttgcatt
       61 caccattata ttctttaaaa gtataagtat cataatcgta ctcatatccg aatgtcacaa
      121 ggtaactgtt gtttttaatg aaccctcctt cttctatttc tgcattgctg atataagcaa
      181 agtattcacc ataaggcact tcatttccat tcctgctcaa atggatttcg cgggatttca
      241 gatggatgga tttgtcagac ggatatgtcc acatataagg gaaagtgctg taccaactcc
      301 ctgcagtaaa aaaaccgaca gcgaaagatt cagagaattg caattcagga aatgtttcag
      361 aaaaaatcat caaaggataa tcatcttttt cctggactcc attaatatcc tcaaaaagaa
      421 acctaagctt tcctgttaac ttttcatctt tctgcaataa aactggattt ttattttcag
      481 ctctccagcc ttgaagctta atactaagat ctccagttct tttatcataa agaatagagc
      541 actgcaaaaa taaaatcaag aaacagtaaa gtatatattt catatcactg cctccgctgt
      601 tgcgaagaat caatcttgct aaaacgcata accgacataa aacatccagt tgacttcact
      661 gagccctgtt cttccctgat actgcagttt cgattctctg atttcattca gtaaatccag
      721 accgtttgca tttcttcctt catatgccag catatcaatt tttgcattca ttgtcctgtt
      781 gaaaaaatgc atctgtccgc attgcaatcc tgtaatgatt cctgccggaa atacccatcg
      841 taacccaaaa tcaagattcc ggaatgcgga attatcaatc caaagcctgt attcccggta
      901 tttattcatt ccgcgccata cgcctggacc gctgacaatt tcattattaa aatacccaaa
      961 ccatccctgc tggaaattat tctccactcc caatccgatg cccaaataaa aaggagaacc
     1021 caggatatgc catccggcat tgacggaacc tttttttgcc tggtatacag ctctgtatcc
     1081 ccctgcaagt gcactcagtt ttcccatccc atactcatgg tcagtctgac tgt
//
LOCUS       DAOQGJ010001763         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108238_length_1133_cov_3.082560, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001763 DAOQGJ010000000
VERSION     DAOQGJ010001763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108238_length_1133_cov_3.082560"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..846)
                     /locus_tag="PK683_21350"
     CDS             complement(13..846)
                     /locus_tag="PK683_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11058.1"
                     /translation="MKDKLQKFLQSHRFHSFQNRLIFHFLTGILFSGTVFCLFEYLPF
                     FGAVLLSAPLCFLFFILIGMRTGNGMTAFGLTLGILIFTGMLKSEKITERDLKNLKAG
                     TLGTLQNPYSPGQEKWDYLVFPKAEIDLKKCTEEKHTSSGGSVKRPYNTVSVYTLCPI
                     AGTSEFAAVCIGFEGVLEERFPCSEFWKNPVEAGRLAEKDIRFQRMLNEIQTKHSVMF
                     SENVQILYPLDPEEFRKKERTSGYKSLLLILFFWRSSLLDSKAKLKFQHFLSLKESII
                     C"
BASE COUNT          344 a          249 c          216 g          324 t
ORIGIN      
        1 tttgcttaaa tatcagcaga taatcgattc ttttaaactt aaaaaatgct gaaattttaa
       61 tttcgctttg ctgtccagta aagaactcct ccaaaaaaac agaataagca agaggctctt
      121 gtacccggaa gtcctttctt tttttctgaa ttcctctggg tccaaaggat aaagaatctg
      181 cacattttcc gaaaacataa cagaatgctt cgtctgaatt tcgttcagca ttctctggaa
      241 acggatatct ttctccgcaa gcctgcctgc ctcgaccgga tttttccaga attcactgca
      301 gggaaatctc tcctccagaa caccctcgaa tccaatacac acagcggcga actcggaagt
      361 gcccgctata gggcagagtg tatacacaga aacagtattg taaggtcttt tcaccgatcc
      421 tccggaagag gtatgttttt cctccgtgca ttttttcaga tcaatctctg ccttgggaaa
      481 taccagataa tcccattttt cctgtccggg agaatatgga ttctgcaggg tgccgagtgt
      541 acctgctttc agatttttca gatctctctc cgtaatcttt tctgatttca gcatccctgt
      601 aaaaatcagt atgccgagtg tgagtccgaa agcagtcatc ccgtttcctg ttctcatccc
      661 gatcagaata aaaaacagga agcacaatgg agcagaaaga agaactgctc cgaaaaaagg
      721 aaggtattca aacagacaga aaacagtgcc tgaaaaaaga atgcctgtaa gaaaatggaa
      781 aatcagcctg ttctgaaaac tgtgaaatct gtgagactgc agaaacttct gaagtttgtc
      841 tttcatataa aactcctttg gccgggatat attttatgac acagcagacg ggagagtcaa
      901 gaaatttttg cgggagtcgc cggactggtc acgattaaat ttgaactgca cctcagaaga
      961 ttctccctgc agaactcatt attcactgaa aatatccgga tgtatttcag aaaaagtgaa
     1021 aaatcttgac cttactgttc ctgactgaat tctgttttta tgaaatcgaa acgctgcttc
     1081 attgcacttt caatctgcat tctgttcatt caatgtcatc agaaaacaga aat
//
LOCUS       DAOQGJ010001764         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108380_length_1132_cov_3.636955, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001764 DAOQGJ010000000
VERSION     DAOQGJ010001764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108380_length_1132_cov_3.636955"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..>1132)
                     /locus_tag="PK683_21355"
     CDS             complement(209..>1132)
                     /locus_tag="PK683_21355"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11059.1"
                     /translation="VTAIATSAGNGSDVTVINNPEGITAGDNGLLYVPNTGASTATNG
                     AFDGHNIFTINTKSSNQIKLLSGKDRPTLRETIDGAQGTNRFYKPEGIAYSASFKKLI
                     AAEAGGNSIREVNITDGSVTTVTGEKASGGSAPTGGTPIATDGPASTARFSSPTGAAI
                     DSKGNIFVADEGNNCIRKIDTSKNVSTFAGSNAGGDSAKGYADGTGTAARFSGPYKIV
                     IDSDDNLYVSDTGNYAIRKITPAGLVTTVAGGKGSGRDDGTASSAKFYKPTGLCIYGN
                     TMYVTDKALARTSTTADLNTDYSAVRKIVNF"
BASE COUNT          264 a          274 c          244 g          350 t
ORIGIN      
        1 aaagcatata tttctcaaaa tctttgaaag caatgacagt gctgtcttcc tgaacctctg
       61 ttactttttc ggcagagcac atacagtctt ctcgaattta ccagccggtc tgttgttcat
      121 atgtgcaagt atttcaaaac tctctctgct atgcatacac ttcctgccgc cgaaaaacag
      181 actgacggca gaatccattc cgtttctgct aaaaatttac aatctttctt accgcactgt
      241 aatcagtatt cagatcagca gtagtgcttg ttcttgccaa tgccttgtca gtaacataca
      301 ttgtattgcc gtaaatgcag agtccagtag gcttataaaa ttttgcgctt gatgcggttc
      361 cgtcatctct tcctgatccc ttgccgcctg ccacagtagt aacgagcccg gcaggagtga
      421 ttttgcggat tgcataattt cccgtatctg atacataaag attgtcgtct gagtcgatga
      481 caatcttgta tggacctgaa aatctggccg cagtgcctgt tccgtcagcg taaccttttg
      541 cgctgtctcc gcctgcattg gaaccggcaa atgtgcttac atttttggaa gtgtcaattt
      601 ttcggatgca gttgtttcct tcgtctgcaa cgaagatatt tcctttggaa tcaattgcgg
      661 caccggtagg tgaagaaaat cttgcagtag aggcaggacc gtcagttgca atcggagtcc
      721 ctccggtcgg agcagatccc cctgaagcct tttcaccggt gaccgtagtc acagatccgt
      781 ccgtgatatt cacttctcta atgctgtttc cgcccgcttc ggccgcaatc agttttttaa
      841 aagaggcaga gtatgcaatt ccctccggtt tataaaatct gttcgttccc tgcgcaccgt
      901 caattgtctc tctcagtgtc ggtctgtctt ttccggaaag aagtttaatc tgattggatg
      961 atttcgtatt gattgtaaaa atattatgtc cgtcaaaagc accgtttgtc gcagtactgg
     1021 ctccggtatt cggaacgtag aggagaccgt tatcacctgc agtaattcct tcagggttgt
     1081 tgatcacggt cacatctgat ccgtttccgg cagaagttgc aattgcagtc ac
//
LOCUS       DAOQGJ010001765         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108389_length_1132_cov_3.342618, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001765 DAOQGJ010000000
VERSION     DAOQGJ010001765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108389_length_1132_cov_3.342618"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..>1132)
                     /locus_tag="PK683_21360"
     CDS             complement(24..>1132)
                     /locus_tag="PK683_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11060.1"
                     /translation="DGVVTKVRRAGIKSITPDRTEKDDPAETSADESLPVPMPAEKPK
                     AESLPEHSFMLTQYMSNDGAFQGSSLYGERDARRNGSRYRDYYQSYFLTTGIDILGLP
                     KPFKFGITMMNPAVDRTNTDSDHFFQTQPGGQDRTALVNSSIQSGTLQYDPNSVKTRK
                     EQNKLLDYLFTRMAYEHETRMGTFYTGFLFIHINEPAYALRGYYLLGWKPPVLSYLNP
                     NFTLNTKMTSEYGGIYQGNHNYRLSLSHEYFKGEKFRVIPSLVIGYQDVNNNASNKRG
                     FSDISPRLQFEYLNFFIALNYMFRTDPDLVDNSYYTPDIGAYPNQSQTDGKTVNPSKA
                     YGYKNQLILNEISAVSPNEFVKRELSQHYQEQQR"
BASE COUNT          282 a          229 c          248 g          373 t
ORIGIN      
        1 tgaatcggat tggattattg ttgtcatcgt tgctgttcct gatagtgctg ggaaagttcc
       61 ctcttgacaa attcattcgg agagacggca gatatttcgt tcagaatgag ctgattttta
      121 taaccgtatg cttttgacgg atttactgtt tttccatctg tctgactctg attcggatag
      181 gctcctatat ccggtgtata gtaggaattg tctacaagat ccggatcagt tctgaacata
      241 taatttaagg ctatgaaaaa attcagatat tcaaactgaa gcctggggct gatgtcgctg
      301 aatccgcgtt tattgctcgc attgttgttt acgtcctggt atccgatgac caggctgggg
      361 atgaccctga atttctctcc tttgaaatat tcatgagaaa gggataatct gtaattatga
      421 tttccctggt atattcctcc atactcggat gtcatttttg tattcaatgt gaaattcgga
      481 ttcagatagg aaagaacagg aggcttccag cccagaagat aataacctct cagagcgtaa
      541 gcaggctcgt tgatatgaat gaacagaaat ccagtataaa acgttcccat cctggtctcg
      601 tgttcatagg ccatcctggt aaaaagataa tccagaagct tgttctgctc ttttctagtc
      661 ttgactgaat tcgggtcgta ttgaagagtt cctgactgta ttgaactgtt tacaagagcg
      721 gttctgtcct gtcctcccgg ctgagtctga aagaaatggt cggaatctgt atttgttctg
      781 tccacagccg gattcatcat tgtgattcca aatttaaacg gtttaggaag cccgagtatg
      841 tcgattcctg tagtaagaaa gtaggactga taatagtctc tgtatctgga accgtttctt
      901 ctggcatccc tttctccgta aagactgctt ccctgaaaag ctccgtcatt tgacatatat
      961 tgagtaagca taaatgaatg ctccggaaga gattctgctt tcggtttttc tgcaggcatc
     1021 ggcacaggaa gactttcatc agctgatgtt tctgcaggat catctttttc tgttctgtca
     1081 ggcgtaatgg attttatgcc tgcccttctt acctttgtga caaccccatc tt
//
LOCUS       DAOQGJ010001766         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108420_length_1132_cov_2.812442, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001766 DAOQGJ010000000
VERSION     DAOQGJ010001766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108420_length_1132_cov_2.812442"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PK683_21365"
     CDS             <1..471
                     /locus_tag="PK683_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11061.1"
                     /translation="DEREEYMLKYVLDLETKDSQSLLNLDGFTDPFSYSMDILENGAL
                     KKKNMDLVETFHYLLGVNVKRYHRREEVQTEKTSFLVQCVEGELRSGEKILIVWRTVP
                     EDAVGANKQLKTYLEKKFTLKDFDKLYINGDSIIENAELLEEHFHKAMFDVRDV"
     gene            560..>1132
                     /locus_tag="PK683_21370"
     CDS             560..>1132
                     /locus_tag="PK683_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004508144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNH11062.1"
                     /translation="MERNIPELPLQEDVETKAVLKQASLAHRRLAELKGISKTIPNQA
                     ILINTLPLLEAKDSSAIENIITTHDEIFRESLFEDFVQSANAKEVQNYSLALRVGFEK
                     VKAGEVLTNNFIMEIQSLIESNRAGFRKLPGTNLKNEQTGEVIYTPPQNAEDIIRLMS
                     NLEKFINDDTISVLDPLVKMAIVHHQFESIH"
BASE COUNT          404 a          182 c          256 g          290 t
ORIGIN      
        1 gatgagagag aagaatatat gctaaagtat gttctggatc tggaaacaaa ggatagccaa
       61 agtctcttga acttggatgg atttactgat ccgttttcat attctatgga tatattagaa
      121 aatggggctc tgaaaaagaa aaatatggat ctggtggaaa catttcacta tcttttggga
      181 gtgaatgtca agagatacca cagacgggaa gaagtgcaaa cggaaaagac ttcctttctt
      241 gtgcaatgtg ttgaagggga actgagaagc ggagaaaaaa ttctgatcgt atggagaacg
      301 gttcctgaag atgcagtcgg cgcaaataaa caactgaaaa cctatcttga aaagaaattc
      361 actttgaaag attttgacaa actctatatc aacggagaca gcatcatcga aaatgccgag
      421 cttctggaag aacatttcca taaagcaatg ttcgatgtaa gagacgtata aaaacccttt
      481 cgtgcagttc gtgcttttga tggttaaaaa aacaggaacc acgaaaaaca cgaaagtcac
      541 gaaaatgcag gagattggaa tggaaagaaa tataccggag ctgcccttgc aggaagatgt
      601 agaaaccaag gctgtattaa aacaggcaag ccttgcacac agacgactgg cggagctgaa
      661 aggaatctct aaaactatac ccaatcaagc aatcctcata aacacgcttc cgctgttaga
      721 ggcgaaggac agcagtgcaa tagaaaatat tataactacc catgatgaaa tttttcgaga
      781 gagtctgttt gaggattttg ttcagagtgc gaatgcaaag gaagttcaaa attattcttt
      841 ggcattacga gttggatttg aaaaagtaaa ggcaggggaa gttcttacaa ataattttat
      901 catggaaata cagtctctaa ttgagtccaa tcgggcagga tttagaaaac ttccgggaac
      961 aaatttaaag aatgagcaaa cgggtgaagt gatttatact ccgccgcaga atgcagaaga
     1021 cattattcgg cttatgagta atttggaaaa atttataaat gatgatacga tttctgttct
     1081 ggaccctttg gtaaaaatgg caattgttca tcaccagttt gaatccattc at
//
LOCUS       DAOQGJ010001767         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108452_length_1132_cov_2.346332, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001767 DAOQGJ010000000
VERSION     DAOQGJ010001767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108452_length_1132_cov_2.346332"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PK683_21375"
     CDS             <1..524
                     /locus_tag="PK683_21375"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH11063.1"
                     /translation="FSGKEPKIFCAMGSSGSAEILRVSLNALKKTDYRVVGAVTSIMD
                     PEEFAPFSDRFYVTRYLPAAQVNELADLAVTHGGQGTLQNSVWAGKPMVGVPFQFEQQ
                     MNLDMMVRAGTGIKIKMHEYTGERLLQEVKRILENPSYSENAKKLSAVFRNTDGADNA
                     AKIIIKYLNERGS"
     gene            complement(641..1102)
                     /locus_tag="PK683_21380"
     CDS             complement(641..1102)
                     /locus_tag="PK683_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11064.1"
                     /translation="MKDLDKKIRKHIEEMNYDAVLLLLENRKDLDPYHTTLKGICIQI
                     TKNPGYTPQDAESCFLKAIEIDGRYVRPYIELGYLYLTALKDKEKAEYFFDQAKEILK
                     DLLVETFMGDFQIKFESRRDASILKMLKDFNDSVMNHKDIIALHDVLKKYK"
BASE COUNT          311 a          226 c          249 g          346 t
ORIGIN      
        1 ttttcagcgg gaaagagccg aaaattttct gcgcaatggg cagttcaggg tctgccgaaa
       61 ttttgagagt gtcattgaac gcactgaaga aaacagatta cagggtcgtc ggtgctgtta
      121 cttccatcat ggatccggag gagtttgctc cattcagtga ccggttttat gtgacgagat
      181 atctgcctgc agcacaggtc aatgaacttg cggatctcgc agtgactcac ggagggcagg
      241 gtactcttca gaattctgtc tgggccggaa aaccgatggt gggtgttccg tttcagtttg
      301 agcagcagat gaatctggat atgatggtca gagcaggaac cgggatcaag atcaagatgc
      361 atgagtatac tggcgaacgt cttctgcagg aagtgaaacg gatactggaa aatccttcct
      421 attctgaaaa tgcgaaaaaa ctgagcgcag ttttcagaaa tacagacgga gccgacaatg
      481 ctgcaaaaat cattataaaa tatctaaatg agaggggttc ctgaacctat tgtttctctg
      541 catttatctg tacatctcag acagagagga tgaaatcgag cctgagtatg ttttaaatgc
      601 gcgagatttc ttttctgtcg aaaatccgga cagatgcagg tcatttatac tttttcagta
      661 catcatgaag agcaataatg tccttatgat tcattacaga atcattaaaa tctttcagca
      721 tcttcaggat gcttgcatcc ctgcgtgact caaattttat ctgaaagtct cccataaaag
      781 tctcaactag gaggtctttc aggatttcct ttgcctgatc aaagaagtat tccgcttttt
      841 ctttatcctt taatgctgta agataaagat agcccagttc gatataaggc ctgacatatc
      901 tcccgtcaat ttcaattgct ttcaaaaaac agctttctgc atcctgtgga gtataaccgg
      961 gattttttgt gatctgaatg catattcctt tcagagtggt gtgatacgga tccaagtctt
     1021 ttctgttttc taaaagcagg agaactgcat cgtaattcat ctcctctata tgctttctta
     1081 ttttcttatc caagtccttc atttgtgtac cttctcaagt cagtgtgaca gt
//
LOCUS       DAOQGJ010001768         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108587_length_1131_cov_2.711896, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001768 DAOQGJ010000000
VERSION     DAOQGJ010001768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108587_length_1131_cov_2.711896"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PK683_21385"
     CDS             complement(<1..627)
                     /locus_tag="PK683_21385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11065.1"
                     /translation="MRDFLKYIPVLLLFLQTMTVFGETPKETPVMQMQSFRELWTTSV
                     NPENSALDLKFSAIPFHFPFEISLTPQYRPVILPLGSDLLYRTPANQTIFRWSGPVFE
                     GNLSAYETSSFPSDRISRTSNDSLRTPAPWMPMPASSYRSPGIVFVYMKKNLGISLDM
                     AFRLSGTLIENTLEQMVSTISLHYSLPFELAKKSYQARFSVNLQSLESS"
     gene            741..>1131
                     /locus_tag="PK683_21390"
     CDS             741..>1131
                     /locus_tag="PK683_21390"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNH11066.1"
                     /translation="MNTTVETLGPCKYDNPAGYEFYTKETSIVMFQTAFPSKEEFFSQ
                     PASERVFLEQSGPMEKIYFNPAEVVAGIVTCGGLCPGINDVIRAIYMELHYRYSVKKV
                     LGFRYGYEGLTEKYGHKPIELVPENTVN"
BASE COUNT          350 a          224 c          257 g          300 t
ORIGIN      
        1 agaactttcc aggctctgca ggtttacact gaatcttgcc tgataggatt tttttgccag
       61 ttcaaacgga agggaataat gaagactgat agtggacacc atctgttcca aagtattttc
      121 tatcaatgtt ccgcttagac ggaacgccat atccagactg atacccagat tttttttcat
      181 atatacaaat actattccag gcgatctgta agaagatgcg ggcataggca tccagggagc
      241 aggagtccga agtgagtcgt ttgaagttct ggaaattctg tctgaaggga aactggatgt
      301 ttcataggcg gacagattcc cttcaaaaac cggtcccgac caccgaaaaa ttgtctgatt
      361 tgcgggggtt ctgtaaagta aatcagatcc aagcggcaga attacaggac ggtattgcgg
      421 cgtgagagaa atttcgaatg gaaaatggaa cggaatggcg gaaaatttaa gatccagagc
      481 agaattttcc ggattgaccg aagtggtcca cagctcccgg aaagactgca tctgcataac
      541 aggggtttct ttaggtgttt ctccgaaaac ggtcatagtc tgcagaaaaa gcagtagaac
      601 tggaatatat ttcagaaaat ccctcattgt acaagcacaa tatccggaaa gcccaaattg
      661 taaaatattt tatccattcc cacttaaaaa tttgcttgaa ctttccaaaa gtttctgtct
      721 gatattccac tgagatgccc atgaatacta cagtagagac attaggtcca tgcaaatatg
      781 ataacccggc agggtacgag ttttatacaa aggaaacaag catagtgatg tttcagacag
      841 cttttccgag caaggaagag tttttcagtc agccggcttc agaaagagtc tttctggagc
      901 agtccggtcc tatggaaaaa atctatttta atccggctga ggttgtcgca ggcatagtaa
      961 cctgcggcgg tctctgccct ggaatcaatg acgtcattcg agcaatttat atggaacttc
     1021 actacagata ttctgtaaaa aaggttctag gcttcagata cggatatgaa ggtcttactg
     1081 aaaaatacgg tcacaagcct attgaactgg ttcctgaaaa cacagtgaac a
//
LOCUS       DAOQGJ010001769         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108727_length_1130_cov_3.249302, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001769 DAOQGJ010000000
VERSION     DAOQGJ010001769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108727_length_1130_cov_3.249302"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..632)
                     /locus_tag="PK683_21395"
     CDS             complement(219..632)
                     /locus_tag="PK683_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11067.1"
                     /translation="MQKRKKKSKVWREITEQKTEFQGILKVLNRFYEAVAEDRKNDSH
                     RFRKSLEKENTEKFRIFLRKFGKYEFLVYAEIRDTETGSRSDSWIHIDGIAEEREQLR
                     KKGNQDHPVFSISCLTDLYLKSSVSAPEFPEELKV"
     gene            complement(718..>1130)
                     /locus_tag="PK683_21400"
     CDS             complement(718..>1130)
                     /locus_tag="PK683_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11068.1"
                     /translation="LIGITVAYTSQGSGYELFPLYSGLSSFLTASFVYWFFCTRTGDF
                     GVWKGAGAGALSGLIAHYFSWYLMLIGQNVCFHFFGGCRSSLNEPPMNVLYGLFGVFP
                     LTFFSLIFLGWVTVPGGAVLGWIFNLIAGRRISP"
BASE COUNT          324 a          250 c          214 g          342 t
ORIGIN      
        1 agtcccctgt tttttttgtc caattgataa gagtagcttc tgtaatcgat tcgcccattt
       61 caggcacttt gatttcgact gccattttga ttccctggat gcatgtattt attaagctgt
      121 aaagctcatc taaatacata cacttgcaga atcagtttat aaggcaatct tttaatgaaa
      181 tctgcaaaaa aaccgaatga atatcaatac agtaagtgtc agactttcag ctcctcagga
      241 aattcaggtg cagagacaga agatttcaga tagagatcag taaggcagct gatggaaaaa
      301 acaggatgat cctgatttcc tttttttctg agctgttctc tttcctctgc tattccgtcg
      361 atgtgaatcc aggaatcgct tctgcttccc gtttcagtat ctctgatttc tgcatacact
      421 agaaattcat actttccgaa tttccgtaaa aagattctga atttttctgt gttttctttt
      481 tcgagtgatt ttcggaatcg atgagaatca tttttcctgt cttctgcaac tgcttcgtaa
      541 aaccggttca gtactttcag tattccctga aattcggttt tctgttcagt aatctcccgc
      601 caaaccttgg attttttttt ccttttctgc atatctgctc ctctgaagta cctttcagca
      661 aatgttcaga agcagagaaa aaaatttaga aatttcaccc tgcagagtat tttttatcta
      721 gggactaatt cgtctgcctg caatcaaatt gaatatccat ccgagcactg caccccctgg
      781 aacagttacc catcccagaa aaatcaggct gaaaaatgtg agaggaaata cgccgaaaag
      841 tccatagaga acattcattg gaggctcatt cagggaactc ctgcatcctc cgaaaaaatg
      901 aaaacagaca ttctgtccga tcagcatcag gtaccagctg aaataatgtg caatgagtcc
      961 gctgagagct cctgcacctg ctcccttcca tactccgaaa tcgcccgttc tggtgcagaa
     1021 gaaccagtag acaaaggatg ctgtcagaaa tgaagacagg cctgaataga gaggaaaaag
     1081 ttcataacct gatccctgag atgtgtaggc aactgtgatg ccgatcagcg
//
LOCUS       DAOQGJ010001770         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108804_length_1130_cov_2.187907, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001770 DAOQGJ010000000
VERSION     DAOQGJ010001770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108804_length_1130_cov_2.187907"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..>1130
                     /locus_tag="PK683_21405"
     CDS             294..>1130
                     /locus_tag="PK683_21405"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNH11069.1"
                     /translation="MLCLERADYKWLLPLCAGVFTVCYSLEKLHPEFVEKLSEETRVG
                     DYVAANTLGFFLLAFVIYYFKKSFDRDRKRLKRKHIMLTEAWENLKVENKRAEDALSV
                     KNQFLANISHEVRTPLNGILGYSEILSDTSLNETQVQYLSIIKKSGNLLLSIVNDVLD
                     ISKIESGKMELESAVFNLREIVEDTSKLAELSIRNSGKNLRLNIEIEENLPHQFKGDS
                     LRISQILLNLLGNAVKFTAEGHIHLRLKKDSRKNALHISVSDTGIGIDEDAVEKLFEP
                     FTQ"
BASE COUNT          347 a          201 c          231 g          351 t
ORIGIN      
        1 aaaaagttca ttgtccatat ttttagcatc gtactgattt cttttctact ttactttctt
       61 ttgcgtaagg tgagataata aataattttc cgcctgagca gaatgttatc attgcggccg
      121 gatctttttt tttcgtatat acatattata cagcaagagt cagaaaaaac tattcattaa
      181 gcatggttct ttttttcagt atttcctggt tcctgattgc aggaggctag ttttacggcg
      241 gaggcattgc gggaaccata ccaatatttt tcgtatattt tctggtgatg agtatgctct
      301 gtctggagag ggcagattat aaatggcttc tgcctctctg tgccggagtg tttactgtat
      361 gttacagtct cgaaaaactt catcctgaat ttgtcgaaaa actgagcgag gagacaaggg
      421 taggggatta tgttgccgct aacaccctcg gtttttttct gcttgctttc gtgatttatt
      481 atttcaagaa aagttttgac cgtgacagaa agcgtctgaa aagaaaacat ataatgctga
      541 ctgaagcatg ggagaatctg aaggtcgaaa ataaaagagc agaagatgcc ctttctgtaa
      601 aaaatcagtt cctggcaaat atcagccatg aggtcaggac tcctttgaac ggcatactag
      661 gatactctga aatactttct gacacctcat tgaatgaaac acaggtgcag tatctttcta
      721 taatcaaaaa aagcggaaat ctcctgctga gcattgtaaa tgatgtactg gatatatcca
      781 aaatagaatc aggaaagatg gaattggaat ctgctgtttt taatctcaga gaaattgtgg
      841 aagatacatc aaaacttgca gagctctcta ttcgaaattc aggaaaaaat ctgcgtctga
      901 atattgaaat tgaagaaaat ctccctcatc agttcaaagg agacagtctc agaatttctc
      961 agatccttct gaatcttctt ggcaacgcag tcaaatttac tgcagagggg catattcacc
     1021 ttcgtctgaa aaaagactct cgaaaaaatg cacttcatat atcagtcagt gatactggaa
     1081 taggaatcga tgaggatgct gtcgaaaaac tttttgaacc gtttactcag
//
LOCUS       DAOQGJ010001771         1129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_108970_length_1129_cov_2.305400, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001771 DAOQGJ010000000
VERSION     DAOQGJ010001771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108970_length_1129_cov_2.305400"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PK683_21410"
     CDS             complement(<1..487)
                     /locus_tag="PK683_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11070.1"
                     /translation="MPKQNKTKTERILTALEYSALLWFEWKFNKKRYDLYQLIFLFYE
                     MSSGSSEYPEAEALERNLRNCLRDLEDELDIQVSLKKKSIECSSSDSAEDLTFGVLSF
                     YLRTAFEERSGEHNDFLIRRFCRYRIFFGEKQTDADFLMSSLYLFVYIKYSMTHKMKM
                     DC"
     gene            complement(569..>1129)
                     /locus_tag="PK683_21415"
     CDS             complement(569..>1129)
                     /locus_tag="PK683_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11071.1"
                     /translation="AKVLKKNALEIDFELLQESIIALKAEAVLNLLNGIKERFDKVID
                     DGTLYKKYSMNPEFIVNFFKVVVKGYVGFEIILSDKLSIFIGLDDYGNRKFFYRFSLK
                     DTNRDSSLSNWIRSRKMTEDGYSDGAVTFYRYFQENLEFSSPAFPFKKFLGDAGKEEI
                     RKFTDELLKKIQEDLGTEIRLSLKTP"
BASE COUNT          337 a          250 c          166 g          376 t
ORIGIN      
        1 aacagtccat tttcatttta tgcgtcatag aatattttat atatacaaat aagtacagac
       61 tgctcatcag aaaatctgca tctgtctgtt tttctccgaa gaatatcctg tatctgcaga
      121 aacggcggat caggaaatca ttgtgctctc cgcttctttc ctcaaaagcg gttctcaggt
      181 aaaacgacaa cacaccgaag gtcagatcct cggcagaatc cgaagaactg cactcgatgg
      241 atttcttctt cagagaaact tgaatatcca gctcatcctc caagtccctg aggcagtttc
      301 tcagatttct ttccagagcc tctgcttccg gatactcaga agaacctgaa ctcatctcat
      361 aaaacaggaa tatcaattga tacagatcat atcttttttt attaaatttc cattcaaacc
      421 aaagaagagc agaatactcc agtgcagtca ggattctttc tgtctttgtt ttattctgtt
      481 taggcataat gggctatttc agttgtttca gcagctgcga aacctttcag attcgcagta
      541 tttgcttttc cgtcaatgct tcaatcattt atggagtttt caaggagagc cggatttccg
      601 ttcctagatc ttcttgaatc ttctttagca actcatctgt aaattttcta atctcttctt
      661 ttcctgcgtc tccaagaaat tttttaaatg gaaatgccgg tgacgagaat tcgagatttt
      721 cttggaaata tctataaaaa gttacagccc catcactata tccatcctcg gtcattttcc
      781 gactacgaat ccagttgctt agactgctgt ctctatttgt atccttaaga gaaaatctgt
      841 aaaaaaattt ccgatttcca taatcgtcca agccaatgaa aatagaaagc ttatcagata
      901 aaattatttc aaatccaaca taccctttta caactacttt aaaaaaatta acaataaatt
      961 ctggattcat actatacttt ttatacaatg ttccatcatc aatgacttta tcaaaccttt
     1021 ctttgattcc attcaaaaga ttaagcactg cctcagcctt aagagcaata atgctttcct
     1081 gaagcagttc aaagtcgatc tccagcgcat tttttttcaa tacctttgc
//
LOCUS       DAOQGJ010001772         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109279_length_1127_cov_3.364739, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001772 DAOQGJ010000000
VERSION     DAOQGJ010001772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109279_length_1127_cov_3.364739"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1127)
                     /locus_tag="PK683_21420"
     CDS             complement(<1..>1127)
                     /locus_tag="PK683_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH11072.1"
                     /translation="PSAIRAPINRYVPKELTEEEAWNTIEDFGKAAEKARQCGFGAVE
                     IMGSEGYLINQFLSPVTNTRNDFFGGNHEKRMNFGIEVMKKVRQYVGKDYPVVYRMSG
                     IDLIPGNPDFEEVLEFAKKLKENGANALNIGIGWHESRIPTISMLVPRGAWAKIAGRF
                     KSKLPDIPIIASNRVNVPETIQRIIKDKEADMVSMARPMLADPDFVNKVRSNLPGRVN
                     TCIACNQACLDHTFKELMVSCLVNPEANRELEFSRLPRGKKSKVVVVGSGPGGMEAAR
                     AASNLGHSVILFEKSSRLGGQLNMASTIPGKFEFKETVRYYKSELNAHEVDVRFNTEC
                     TVEILESLNPDAVIFATGVRPREFSLPGLEKKKTGTYLEYL"
BASE COUNT          272 a          291 c          218 g          346 t
ORIGIN      
        1 cagatattcc aggtatgttc ccgttttttt cttttccaaa ccggggagac tgaattcacg
       61 cggacggacg cccgttgcaa aaatcacagc atcagggttc aaggattcca gaatttccac
      121 agtgcattca gtattgaacc tgacatccac ctcatgtgca ttcagttcac ttttatagta
      181 gcgtacagtt tccttgaatt caaatttacc cggaattgta gaggccatat tgagctgtcc
      241 cccgagcctg gaagacttct caaaaagaat gacactgtgt cccagattcg aagcagctct
      301 tgctgcctcc attccccctg gacctgaacc gacaactact actttgctct tttttcctcg
      361 cggaagtctg gagaactcaa gctctctgtt tgcttccgga ttcacaaggc aggataccat
      421 cagttccttg aaagtgtgat ccagacaggc ctgattgcag gcgatgcatg tattgaccct
      481 gcctggaaga ttgcttctga ccttgtttac aaaatcagga tcggcaagca ttggtctggc
      541 catactgacc atatcagctt ccttgtcctt gatgattctc tgaattgttt ccggaacatt
      601 cactctgttc gacgcaataa taggtatgtc cggaagtttg cttttgaatc tgccggcaat
      661 ttttgcccag gctcctctcg gaaccagcat actgattgta ggaattctgg actcatgcca
      721 tccaatgcct atattaagtg catttgctcc attctctttc agtttttttg caaattccag
      781 aacctcttca aaatcaggat ttccaggaat cagatcaatt cccgacatcc tgtagacaac
      841 aggataatcc tttccgacgt actgacgcac ttttttcatg acttcaatac cgaaattcat
      901 gcgtttttca tgattgccgc cgaaaaagtc atttctcgtg tttgtcacag gagaaagaaa
      961 ctgattaatt aaataccctt cgcttcccat aatctcaact gcaccgaacc cgcactgtct
     1021 ggctttctct gcagcttttc cgaaatcttc aatggtgttc catgcttctt cttctgtcag
     1081 ttctttcgga acatatctgt tgatcggtgc acgtatcgct gacggcc
//
LOCUS       DAOQGJ010001773         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109290_length_1127_cov_3.188433, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001773 DAOQGJ010000000
VERSION     DAOQGJ010001773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109290_length_1127_cov_3.188433"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1127)
                     /locus_tag="PK683_21425"
     CDS             complement(<1..>1127)
                     /locus_tag="PK683_21425"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNH11073.1"
                     /translation="DPRNREELEKEYIEFLKEDVQTKEGLERFTEKWSELAAIVDEAG
                     SLSYVGMTSDTKNEKAEKDYLHLVENIFPLCQVYEDRVKEKFLSSPALNQLNSEYYAL
                     FIKQVRSEKEIYREENISLHTKDSLLAQNYQKITGAWMAEFEGKRRTMQEINVFLESP
                     DRDLRERAYRTRVGVHLQDAENLENLFDEMLSTRAKIASNAGFSNYRDYKFVAGGRFD
                     YTPSDCLRFHDAIAETISPLLSEWTKEKCSKLGISSLRPWDISVDPDAKEQIRAFGGE
                     EEFLEKTGKIFDSIHPRLGSYFQFMRKEKLLDLSSREGKAPGGYMTELTERRVPFIFM
                     NAVGTKRDVETLLHEAGHSFHAFLCREQILKGYRSSPLEFA"
BASE COUNT          269 a          296 c          215 g          347 t
ORIGIN      
        1 ccgcaaattc cagaggggaa cttctgtacc ctttcagaat ctgttccctg cagagaaatg
       61 catgaaagga atgccctgcc tcgtgcagaa gagtctccac atcccttttc gtcccgactg
      121 cattcataaa aatgaaaggg actctccttt ctgtcagctc cgtcatgtac ccgcccggcg
      181 cctttccttc gcggctggag agatccagaa gtttttcctt tctcataaac tgaaaataac
      241 tgccgagtct cggatgaatc gaatcaaata ttttcccggt tttttccaga aactcttcct
      301 cacctccaaa agccctgatc tgctcttttg catcaggatc tacggaaatg tcccagggac
      361 ggagagaact gatgccgagc ttactgcatt tttccttagt ccattcggaa agaagcgggg
      421 aaatcgtctc tgctattgca tcatgaaaac gcaggcagtc agacggagta tagtcaaacc
      481 tgccccctgc gacaaattta tagtctctgt aattggaaaa acctgcattg gaagcgatct
      541 ttgccctggt actgagcatt tcatcaaaga gattttcgag gttttccgca tcctgaagat
      601 ggacccctac ccttgtcctg tatgcccttt ctcttaagtc tctgtcgggg ctttccagaa
      661 aaacattgat ctcctgcatt gttcttcttt ttccttcgaa ctcagccatc caggcacctg
      721 tgattttctg atagttctga gcaagaagag aatctttcgt atgcagactt atgttctcct
      781 ctctgtaaat ttccttttct gatctgacct gttttataaa aagggcatag tattctgaat
      841 tcagctgatt cagtgcaggc gaggaaagaa atttctcctt taccctgtct tcatagacct
      901 gacacagagg aaagatattt tcaaccagat gcagatagtc cttttctgcc ttttcatttt
      961 tagtatcaga ggtcatcccc acatagctca gtgaacccgc ttcgtccaca atcgctgcca
     1021 gttccgacca tttctctgta aatctttcca gcccctcttt tgtctgaacg tcttccttca
     1081 ggaactcaat gtactctttt tcgagctcct ctctgtttct tggatca
//
LOCUS       DAOQGJ010001774         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109361_length_1127_cov_2.129664, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001774 DAOQGJ010000000
VERSION     DAOQGJ010001774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109361_length_1127_cov_2.129664"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..736
                     /locus_tag="PK683_21430"
     CDS             47..736
                     /locus_tag="PK683_21430"
                     /inference="COORDINATES: protein motif:HMM:NF026198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNH11074.1"
                     /translation="MIKYSLVKSRLSTSERAPYVARTVQSDAVSFEDFIRALSFGSTA
                     TAADVRAVMENLERVCTENLAQGRSINLGFCILKSAIKGHFTGPEDSFSEEKNWIKIR
                     SKFSPSFEKKVSLAAKMGRIAGVKTVPVVTSLVNHTATVENKIRAADLMTLRGENLKF
                     DSTAPEQGIFFSGNGGEEKVTEYSLVKAKSAIFKIPAGLNSGSSYKLLVRNLFGKELR
                     TGEMDSSILAE"
BASE COUNT          342 a          228 c          241 g          316 t
ORIGIN      
        1 tgtttgaatt tattcgaatg agataaaaat gattaaagga tagaatatga ttaaatattc
       61 acttgtaaaa agcagacttt ctacttcaga aagagccccc tatgttgcca gaacggtgca
      121 gtccgatgca gtttcttttg aggatttcat ccgggctttg tcttttggat ccactgcgac
      181 ggccgcagat gtacgggcag tgatggaaaa tctggaaaga gtctgcacgg aaaacctggc
      241 tcagggcaga agcatcaatc tgggtttctg tattctgaaa tctgccatca aaggacattt
      301 tacaggtccg gaagattctt tttccgaaga aaaaaactgg attaaaatcc gttccaagtt
      361 cagtccctcc ttcgagaaaa aagtgagtct tgcggcaaag atgggcagaa ttgcaggagt
      421 aaaaactgtt cctgtcgtta ccagtctcgt gaatcatacc gcaactgttg aaaataaaat
      481 acgggcagca gatctgatga ctctccgagg agaaaatctg aaatttgaca gcactgcacc
      541 tgaacagggg atttttttct cgggaaacgg gggagaagag aaagtgacag agtattctct
      601 ggtaaaagcc aagtcagcga ttttcaaaat acctgcagga ctgaattcag gaagcagtta
      661 caaacttctg gtcagaaatc ttttcggaaa agaactgaga acaggggaaa tggactcttc
      721 cattctggca gaataatcca tctcccgcaa aaccacttta cctgtctgat ttttgccgaa
      781 atcagacagg tcttttctca gactcaactg cgtgctgata aaatcaagtc aaaaacacag
      841 gcatagactg agacttccgt tctttctttt tttcagcaga tcaaatcatt aaaatttaat
      901 ttcacacctt tcagataaaa aaaatgagcc tttcattatt tctgaactca gctgagaaaa
      961 tttcttgtga gcctgagttt tgatccgagt gagccttgta aaattttttt tgagcctgag
     1021 aaaatttctg atgagcctga gttttgatcc aagtgagctt tgtaaaattt tttgaggaag
     1081 gcggacctca ccccgcctcc ggcacccctc tccatttctg acgtcat
//
LOCUS       DAOQGJ010001775         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109453_length_1126_cov_3.871148, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001775 DAOQGJ010000000
VERSION     DAOQGJ010001775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109453_length_1126_cov_3.871148"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PK683_21435"
     CDS             complement(<1..1079)
                     /locus_tag="PK683_21435"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH11075.1"
                     /translation="MKNRIVFGVGLTMYDMLSFDRNRDVADDWKIPGSRYLNREDTMA
                     AEPNIVKENLHGSYLYYDYANLNPERHTSQFIFSARTRGSLGFNYTEVLSIEKKGEIF
                     EITVWDKVKSRKYLFRSRTLVNCGGPWADYIDTAALKQNPNPLVRSKGIHIITRKICG
                     DNTIAVQTREGAHLFIIPWRGKTIIGTTDVIYDGHPDEFKVTLKDITDLIASVRNYTE
                     HSVSLEDVEMFYGGLRPLVSDGKSSKETYNASRKTEITDHAASGFKGYYTALGGKYTT
                     SRDVAEKAVNKICSYLPGKFRACQTMDIPVLGGYIAGIREAVVDIKKKYPHISEEKAY
                     VLSRRYGSHAEEILKLDRKTKDEIRL"
BASE COUNT          285 a          270 c          223 g          348 t
ORIGIN      
        1 agcctgattt catcctttgt cttccggtcg agtttcagaa tctcttcagc atggcttccg
       61 tatcttctcg aaaggacata ggccttttcc tctgaaatat gaggatactt ctttttgatg
      121 tccacgacag cctctctgat cccggcaata taaccgccca gcacaggaat gtccatcgtc
      181 tgacaggctc tgaattttcc gggaaggtat gaacagattt tattgactgc tttttctgca
      241 acgtccctgc ttgtagtata ttttccgccg agtgcagtat agtatccctt gaagccggat
      301 gcagcgtgat ctgtaatctc tgtcttcctc gaggcattgt atgtttcctt ggatgacttt
      361 ccgtcactga ccaagggtct cagccctccg taaaacattt ccacatcttc caatgaaaca
      421 ctgtgttcag tatagtttct gacagaggca atcagatcag taatatcctt cagagtgact
      481 ttaaattcat caggatgtcc gtcataaatg acgtcggtcg tgccaattat cgtctttcct
      541 ctccacggaa tgatgaaaag atgagcacct tcccgggtct gcactgcaat cgtattgtca
      601 ccgcagattt tccgtgtaat gatgtgaatc ccctttgaac ggacaagagg attgggattc
      661 tgcttaagtg ctgcagtgtc aatatagtcc gcccagggac ctccgcagtt gacaagagtc
      721 cttgaacgga agagatactt tctgcttttc actttatccc atacagtaat ctcaaaaatc
      781 tcacctttct tttcaatgga aagcacttca gtataattaa atcctaatga tcctctggtt
      841 cttgcagaaa aaataaactg actagtatgt ctttcaggat tcagatttgc ataatcgtaa
      901 taaagatagg aaccatggag gttctccttc acaatgttcg gttctgcagc cattgtatct
      961 tctctgttca ggtacctgct cccgggaatt ttccagtcgt cagcaacatc cctgtttctg
     1021 tcaaaggaaa gcatatcata cattgtgagt ccgactccaa acacaattct gttcttcatt
     1081 gaatactgag gaagaataaa ccccatcggt tttactgcat ggggac
//
LOCUS       DAOQGJ010001776         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109473_length_1126_cov_3.273576, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001776 DAOQGJ010000000
VERSION     DAOQGJ010001776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109473_length_1126_cov_3.273576"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1126
                     /locus_tag="PK683_21440"
     CDS             <1..>1126
                     /locus_tag="PK683_21440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH11076.1"
                     /translation="VTETLRLLQNKTQAINAGDFSPAVIMTDLREFAELSKSIDAMSS
                     RLKIQFIDLNLEKEKFNLVLQNLKEGLFALDAKGNIQFVNKSVPKSFVSENSIGKHIS
                     KAVPDRRIVKFLEERLMTGIEGRMNFDYRKKHYSIRVHILKSESRGSLLIGVILDRTE
                     DKKNELLREEFFQNASHELKTPITSIKGYAETLYDRLNADEKSPEKKFLGAILRNTDR
                     MVTLVDDMIKITQLESSEFAFQPEEFLLEDLMENLKYSVEGLLERKRQRLEIIIPEKF
                     SVFGDMILLEHLLLNLIQNASAYSAEDTVIRVEASQDEYSQEIKVTDQGIGISDENIS
                     RIFERFYRADTARSRKEGGTGLGLSIVKQIAKLHSGWIDV"
BASE COUNT          364 a          208 c          260 g          294 t
ORIGIN      
        1 atgtcacgga aacacttagg cttcttcaga acaagactca ggcaatcaat gcaggggatt
       61 tttctcctgc agtaataatg accgatctcc gtgagtttgc cgaactttcc aaaagcattg
      121 atgcaatgtc ttcaagactg aagattcagt ttattgatct gaacctggaa aaggaaaaat
      181 tcaatctggt tcttcagaat ctgaaagaag gacttttcgc acttgatgcc aagggaaata
      241 tacagtttgt aaacaagtct gttcctaaat cattcgtttc tgaaaactcg attggaaagc
      301 atatcagcaa ggctgttcct gacagaagaa ttgtaaagtt tcttgaagaa aggctgatga
      361 caggaatcga aggaagaatg aatttcgatt atagaaaaaa gcattattcc ataagggttc
      421 atatcctcaa gtcggaatca agaggttccc tgctgatcgg tgtcatcctg gacaggacgg
      481 aagacaagaa aaatgaactt ctcagggaag agttttttca gaatgcatct catgaactga
      541 aaaccccgat tacttccatc aaaggctatg cggaaaccct gtatgacaga ctgaatgcag
      601 atgaaaagtc tcctgaaaaa aaattccttg gagccattct cagaaatacg gacaggatgg
      661 tgacgcttgt agatgatatg atcaagatca ctcagctgga aagctctgaa tttgcatttc
      721 agcctgaaga gtttctgctc gaagatctga tggaaaatct gaaatattct gtggaaggac
      781 ttctggagcg caaaaggcag agattggaaa ttatcattcc tgaaaaattt tctgtcttcg
      841 gggatatgat acttctggaa catctgctcc tgaatctgat tcagaatgcg tctgcctatt
      901 ctgccgaaga taccgtgatc agagtagagg catcacagga tgagtattca caggaaatca
      961 aagtaaccga tcagggaatc gggatcagtg atgaaaatat cagcaggata tttgagcgtt
     1021 tttacagggc agatactgcc agatcgcgaa aagagggagg aactggactt gggctttcca
     1081 ttgtaaagca gattgccaag cttcattccg gatggatcga cgtaaa
//
LOCUS       DAOQGJ010001777         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109511_length_1126_cov_2.644258, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001777 DAOQGJ010000000
VERSION     DAOQGJ010001777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109511_length_1126_cov_2.644258"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK683_21445"
     CDS             complement(<1..277)
                     /locus_tag="PK683_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH11077.1"
                     /translation="MITSLLDFALKNRGITLIAAGAIFVAGTFSWLNLRKEAYSDIAD
                     TQVRLIAKFPGRSALEVEERVTLPIERVLNAIPKVIVRRSRTINGLVV"
     gene            complement(274..1029)
                     /locus_tag="PK683_21450"
     CDS             complement(274..1029)
                     /locus_tag="PK683_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11078.1"
                     /translation="MDTKKDIKSLFLILSCFFCLGTAAEENRSNAEYLEENSKIVNDK
                     RERAGALEKEGKDRREEPDFMTNGRKENFFVAGFSAGSPAGLNFNAGWYFDRFVIRGS
                     GMSYNSNWNGAQLDLGYSFYKTKEVIMGFSLVGGAFRANPFDPQTGSGGQNKYKRFDF
                     PGYENQPPTLTDNLIRSYIASQNSDLAALLEYKYRTRQYGKFSQNYIGLTYDVYLDGF
                     FLQLGAGYGQGSYRNPQLLIQVGYLFDFGKSQK"
BASE COUNT          285 a          272 c          222 g          347 t
ORIGIN      
        1 atacaaccag accgttgatt gttctcgaac gtctgacgat gactttcgga atcgcattca
       61 gaaccctttc tattggaaga gtgactcttt cttccacttc gagtgcggat cttccaggaa
      121 attttgcaat cagtcttacc tgtgtatctg caatgtctga ataggcttcc tttctgagat
      181 tcagccagct gaaagttccg gccacaaaaa ttgcgccggc tgcgatgagg gtgatccctc
      241 tgttttttaa agcaaaatca agaagacttg taatcatttc tgcgacttcc cgaaatcaaa
      301 taaatatccc acctgaatca ggagctgcgg gtttctgtaa ctgccctgac cgtaaccggc
      361 tccaagctga agaaaaaaac cgtccagata aacatcataa gtcagaccta tatagttctg
      421 tgaaaacttc ccatactgtc ttgttctgta tttgtattcc agaagggcgg caaggtccga
      481 gttctgagag gctatatagg atcggatcag attgtccgtg agggtagggg gctgattttc
      541 ataacctgga aaatcaaatc ttttatattt gttctgaccg cctgatcctg tctgaggatc
      601 aaaaggattt gctctgaatg ctccgcccac taatgaaaat cccatgatga cttccttggt
      661 tttatagaag gaatatccca gatcgagctg agcaccgttc cagttggaat tgtagctcat
      721 cccggaacct ctgattacga atctgtcaaa ataccatcct gcattgaagt tcagtcctgc
      781 tggacttcct gcagaaaagc ctgccacgaa gaagttttcc tttcttccgt ttgtcatgaa
      841 atcaggctct tcccttctgt cctttccttc tttttcgagt gcgccggctc tttcccgttt
      901 atcatttacg attttggaat tttcctccag atattccgca ttcgatctgt tttcttcagc
      961 agcggttccg aggcagaaaa agcacgacag aattaaaaat aatgatttta tatctttttt
     1021 agtatccaat gctcagtcct ttgagaagca ttgtgcctga gactacaatc tcatcattcc
     1081 agctcagccc ctgcattact tccactttat ccctgtctga attgcc
//
LOCUS       DAOQGJ010001778         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109515_length_1126_cov_2.606909, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001778 DAOQGJ010000000
VERSION     DAOQGJ010001778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109515_length_1126_cov_2.606909"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..405)
                     /locus_tag="PK683_21455"
     CDS             complement(187..405)
                     /locus_tag="PK683_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11079.1"
                     /translation="MEERLFPKTREEIIDTEMKKLKLPSFGKFLIESVLDGRIKEESL
                     ICCHSDCSLCNEKVSQCLSEIKKQLNEK"
     gene            complement(410..>1126)
                     /locus_tag="PK683_21460"
     CDS             complement(410..>1126)
                     /locus_tag="PK683_21460"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH11080.1"
                     /translation="QTSHICYLLLQRSIQSRENRETVFEAADSGGVIKQMDIRGSRYI
                     DFLIPGLLALGVMNSCLWGTAWTLIELRMKKLLRRMMATPLPKSYFLFSHLLMRMILS
                     GLEFIFLMIFGYFVFSVEVQGSLTALFLLFLAGNLAFTGIAVLISSRVANSQIGNGLI
                     NLVSFPMTICSGVFFSYHGFPDLFRNIVKYFPLTVLADGVRAVFTEGAGLQESGIPIL
                     ILGITGLIFFGAGIRLYKWD"
BASE COUNT          343 a          243 c          229 g          311 t
ORIGIN      
        1 tgtcatatag gagtttgcat cttttacaag aactgcatgt ttgttttttt cattttctga
       61 acttggcttg cagaactgaa ctgcagagag gaatgccaaa gctaaaaaag tgaatgtaat
      121 ttttttcata gtcacctcat ttataaatcc agaaacccct gtttccattt atatgtcaat
      181 tatttattat ttttcattca gctgtttttt gatttcagaa agacactgag aaactttctc
      241 attgcagaga gaacagtcgg aatgacagca gatgagcgat tcctccttga ttcttccatc
      301 cagaacagat tctatgagga attttccgaa agaagggagc ttcagcttct tcatttcggt
      361 gtcgatgatt tcttctctgg ttttcggaaa aagtctttct tccatattat cagtcccatt
      421 tatacagtct gattccggcg ccgaagaaga taagtccggt gattcccaga atcagtatag
      481 gtatcccgga ttcctgaaga cccgctcctt ccgtaaatac tgccctcact ccgtcagcaa
      541 ggactgtcag aggaaagtat tttacaatat tcctgaaaag atccgggaat ccgtgatagg
      601 aaaaaaaaac tcctgaacag atagtcatcg gaaaggaaac caggttgatg agaccgttcc
      661 ctatctgaga atttgcaacc ctcgaagaga tgagaacagc aattccggtg aatgcaagat
      721 tgcctgcaag aaaaagcagg aagagtgcag tcagtgaacc ctgaacctcc actgaaaata
      781 caaaatatcc gaatatcatt aaaaatataa actcaagacc ggaaagaatc attctcatca
      841 gaagatgaga aaaaagaaaa taggatttcg gaagaggagt cgccatcatc cgtctcagaa
      901 gtttcttcat cctgagttcg atcagagtcc aggcagtccc ccagagacag gaattcatca
      961 cgcccagtgc aagaagtcca ggtatcagaa aatcaatgta cctgcttccg cgtatgtcca
     1021 tctgtttgat gaccccgccg gaatcagcgg cctcgaagac agtctccctg ttttctctgc
     1081 tctgtatgct tctctgaaga agaagatagc agatatggct ggtctg
//
LOCUS       DAOQGJ010001779         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109655_length_1125_cov_3.905607, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001779 DAOQGJ010000000
VERSION     DAOQGJ010001779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109655_length_1125_cov_3.905607"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK683_21465"
     CDS             complement(<1..351)
                     /locus_tag="PK683_21465"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH11081.1"
                     /translation="MGNPNIKEMISSNFLADTDSKEYKLGDIIMSEGDASNETMYFIV
                     SGDMTVLKNRGSHQEEINQLPAGEFFGEIALVSDQPRTATVKVKSDTARVISFSKSKF
                     IQQTRTNPTLMFSIL"
     gene            complement(497..>1125)
                     /locus_tag="PK683_21470"
     CDS             complement(497..>1125)
                     /locus_tag="PK683_21470"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNH11082.1"
                     /translation="RGTYVCTADPPGTIYDFGGFPEQLYRLKYSCPGSPSLAKEISEK
                     VQGFSIGKTEDWGLDHGTWEILIQMYPKADIPVLQMSINYEKDNADYHYRLGAALRFL
                     REEGVLILASGNIVHNLGTIRFNDTTGYPWAESFDGTVEKLILERNIKALNSWKTLGE
                     PALKSVPSPDHYYPLMYILGASTEKDKISFPVKGLTMGSISMRSVLFS"
BASE COUNT          297 a          257 c          215 g          356 t
ORIGIN      
        1 gagtatggaa aacataagag tgggatttgt acgagtctgc tgaatgaatt tgcttttgct
       61 gaaactgatg acccttgcag tgtctgattt tacttttaca gtggcagttc tgggctgatc
      121 cgatacaagc gcaatttccc caaaaaactc gcctgcagga agctgattga tttcttcctg
      181 atgactgcct ctgtttttca gtacagtcat atctcctgat acaataaagt acattgtttc
      241 attggaagca tccccctcag acataattat gtcgccgagt ttatattcct tagaatcagt
      301 atcagccaga aaattactgc tgatcatctc tttaatgttc ggatttccca ttttttacaa
      361 tttccccaaa catcttcatg gatctgtgtt ttctttcaag tctttttcga tttttcaaaa
      421 ggtccggtaa aaaaagcatc cgtatatgca aaagcagatt atgcctgaat ttctctcttt
      481 ctctgtttct ctaaattcag ctgaacagta ccgaacgcat cgagattgaa cccatagtca
      541 ggcccttaac aggaaaactg attttgtcct tttcagtcga agcccccaga atatacatca
      601 ggggataata atgatcgggg gaaggaactg atttcaaagc aggttcgccg agagtcttcc
      661 agctgtttaa tgcttttata ttcctttcca gaatgagttt ctccactgtc ccgtcaaaac
      721 tctccgccca gggatatcct gtcgtatcat taaaacgtat tgttcccaga ttatgcacga
      781 tgtttccgct tgcaagaatc agcactccct cttctctgag aaatctcaaa gctgcaccca
      841 gtctgtaatg atagtctgca ttgtcttttt cataattgat gctcatctgc agaacaggaa
      901 tatctgcctt aggatacatt tgaatcagta tttcccaggt accatgatcc agaccccagt
      961 cctcggtttt tcctatgctg aatccctgaa ccttttccga gatttccttt gcaagagaag
     1021 gagaaccggg acagctgtat ttcagtctgt acagttgttc aggaaaaccg ccaaaatcgt
     1081 aaattgttcc gggaggatcg gcagtgcata cataggttcc tctgg
//
LOCUS       DAOQGJ010001780         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109672_length_1125_cov_3.353271, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001780 DAOQGJ010000000
VERSION     DAOQGJ010001780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109672_length_1125_cov_3.353271"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..524)
                     /locus_tag="PK683_21475"
                     /pseudo
     CDS             complement(9..524)
                     /locus_tag="PK683_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     gene            complement(526..1017)
                     /locus_tag="PK683_21480"
     CDS             complement(526..1017)
                     /locus_tag="PK683_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HNH11083.1"
                     /translation="MKYTVKHTFNFPLKDVLAARENRYKHLDKFPDLQNVTMIEERQE
                     AEKVFQKRKVSLAKSLPAVLQSFLKDPNLIEESVFDLNTSIHTFRFYPPGNEKVVTIT
                     GKSIYKAVDDKTSERVYDVEVKSEVFLVSALVEAAIEEIHKHSLEKDKASILKFLSDG
                     SIV"
BASE COUNT          319 a          239 c          216 g          351 t
ORIGIN      
        1 tttcatagtg gaggattctc cggtttttca acaagattga caatcctgtc cccatccagt
       61 tccactgagt aatttttcct gatcctggaa atgataggtg tatagacaac gccaatcgaa
      121 gccagcagat caggatgaga tttcagagtt gtaaggaaat tttcatgatc agtcttataa
      181 taaaactcat ctcccaaaat tgtaatgaaa ggcgccgtta tttttgattc aagggatgca
      241 acatcgctgg caagtccctt agtcgtccag tcacagggtt caatggagat cttaggatat
      301 tcatacttca tcctttcgat ttcttcaatc acctgctcct tcagatgacc gaccaggata
      361 taaatctttt cgacaccgaa tttttcctgc atcagagcaa cattatgaga aagaatagac
      421 ttgttgttga ccaggaaaag aggcttggga ataaaacttg tcctcgggta ggcacgagtc
      481 cccttccctg cagccgcaat aacaccgact ttcacttttt tcatttcaga ctatgctgcc
      541 gtcagaaagg aatttgagaa tggaggcctt gtctttttca agactgtgct tgtgaatttc
      601 ttcaattgct gcttcaacga gtgcactgac gagaaatact tccgatttga cttccacatc
      661 gtagactctc tcgcttgtct tatcatctac tgccttgtaa atgcttttcc ctgtaatcgt
      721 gacgaccttt tcgtttccag gaggataaaa gcgaaacgta tgaatgctcg tattcaggtc
      781 aaaaaccgat tcttcaatca gattcgggtc tttcagaaat gactgaagca ctgcaggaag
      841 ggactttgcc agactgactt ttcttttttg gaaaaccttt tcggcttcct gtctttcctc
      901 aatcatcgtg acattctgaa gatcaggaaa tttgtccaaa tgcttatatc tgttttcgcg
      961 ggctgcaaga acatctttca aaggaaaatt gaaagtgtgt ttaactgtat atttcatcaa
     1021 gttcaaatga ccggaacaga cagttttttg caagagaaat ccgctaatta tttaacttta
     1081 tgcaaaaaaa atttgacaga aatacacagt actgaattcc ttgta
//
LOCUS       DAOQGJ010001781         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109699_length_1125_cov_2.773832, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001781 DAOQGJ010000000
VERSION     DAOQGJ010001781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109699_length_1125_cov_2.773832"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..>1125)
                     /locus_tag="PK683_21485"
     CDS             complement(141..>1125)
                     /locus_tag="PK683_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11084.1"
                     /translation="QAAKYRYAKSTAVSGGKRLEVWWNTSSAIYGTKKAFEINFKEEG
                     NSVTGYLWARTLNSDNATLATVYVKFSADTSTGKKTTAVAMKGFYSGYTKLPENGYYY
                     IKEENNLASLDGGFTLTNYNPSITGVTAFSRAYLFTAAGTAARGAVKIAFPRIDSTDV
                     TVWDTGSVANLPEVWTDWILYSLNAQSQLSSIKSLSSSCSSLTAPSTDNPQTNSGSTA
                     SEFKTCIDALYKAYPANSGIKDVVYITRILNPAFYSVSGTNVFLDATETARDTTWDTV
                     TAKLQSKVRNGDPGDGYNANFTAASLATMDFATGTGLPSEAKWTTDDTASF"
BASE COUNT          293 a          244 c          237 g          351 t
ORIGIN      
        1 cctgcaggaa gtaaagagtt ttttaataaa attttcgtgt atctcttttt ttacgtatag
       61 gtgtgtctca aatcaaaaaa atccgattca gttcaaagcc agtgcaaaaa gccgcaccac
      121 tatgcggctt tttattcagg tcagaatgat gctgtgtcgt ccgttgtcca tttagcctca
      181 gaaggaaggc cggtacctgt cgcaaaatcc atagtggcca gacttgcagc agtgaagttc
      241 gcattgtacc cgtctccagg atctccgttt ctgactttgg actgaagttt tgcagtgact
      301 gtatcccagg ttgtatctct ggcagtttca gtggcatcca aaaatacatt cgttccggaa
      361 actgaataga atgcaggatt caggattctg gtaatgtata caacatcttt gatccctgaa
      421 ttcgccggat atgctttgta aagggcatcg atgcaggtct taaattcaga agcagttgaa
      481 ccgctgtttg tctgaggatt gtcagtgctc ggtgctgtaa gactggaaca tgaagaactt
      541 aaactcttga tagatgaaag ctgggactgc gcattcagac tgtaaagaat ccagtcagtc
      601 catacctcag gtagattggc cactgaccct gtgtcccata cagtaacatc agtactgtct
      661 attctaggaa aagcaatttt tactgctcct ctggctgctg tacctgcagc tgtaaataaa
      721 taagctctgc tgaaagctgt tacacctgta atactgggat tgtaatttgt cagggtgaat
      781 cctccgtcca gacttgccag attattctct tccttaatgt aataataacc gttttcagga
      841 agttttgtgt acccgctgta aaaacctttc attgcaactg cagttgtttt tttgccggtg
      901 cttgtatctg cagaaaactt aacatacaca gtggccagtg ttgcattgtc tgaattcaga
      961 gttcttgccc agagatatcc tgtaacagaa tttccttctt ccttgaaatt gatctcaaac
     1021 gccttttttg taccgtaaat tgcagaactt gtattccacc atacttccaa tctttttccc
     1081 ccggaaacgg cagtagattt ggcatagcgg tatttagctg cctgc
//
LOCUS       DAOQGJ010001782         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109729_length_1125_cov_2.465421, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001782 DAOQGJ010000000
VERSION     DAOQGJ010001782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109729_length_1125_cov_2.465421"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>1125
                     /locus_tag="PK683_21490"
     CDS             192..>1125
                     /locus_tag="PK683_21490"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNH11085.1"
                     /translation="MSNKSAGSKISSRQRRAVGMEKYLSALGRVICVFAALSVNEGVL
                     SDSEKPAEKKTADSFAEKFNSDDFPGIFEMFSPSLQKSLTLSKTEEFLKGVKLGSGRM
                     KKKQFQGYSKNAFASYLAVCEYSKFTLELVLDKDSRISGYYIRTVPAAETDFPKIERN
                     ISRIQFPAEGVWSTTWGGDTKELNYHVEKKFQKNAFDFVIRDERNSSYKNDGKKNEDY
                     YAFGKELFAPADGIAVYVKDGFPDNKPGEKTDKPNEVIIRTENGEYVVLLHFKKNSIA
                     VKQGEKIKRGQLLGLVGNSGFSSEPHIHFHMMNGF"
BASE COUNT          367 a          203 c          265 g          290 t
ORIGIN      
        1 tactgaaaag tattctgcgg agaaaaaact gtctttttcc gctgtctgac gatatatcat
       61 ttgtctggac aagtgctcac tctgtcttcc ggaaaatgtc ttgtttacag cataaggccg
      121 gaaacggatt gaatagatct tgcaggcgcc cagtacccct caagaaactt ttttactgaa
      181 acggaaagag agtgtctaat aaatcagctg gctcaaaaat ttcgagcagg cagagaaggg
      241 cggtaggaat ggaaaaatat ctgtcagcat tgggaagagt catctgtgtt tttgcagcat
      301 tgtctgtaaa tgagggtgtc ctcagtgatt cggagaaacc tgcagaaaaa aagactgcag
      361 acagttttgc agaaaaattc aattcagatg attttccggg aatatttgaa atgttcagtc
      421 cttctcttca gaaatcactg acactgagca agacggaaga attcctgaaa ggcgtcaagc
      481 ttggttcagg caggatgaaa aagaaacagt ttcaggggta ttccaagaat gcatttgcct
      541 cctatctggc agtatgtgaa tactcaaagt tcacactgga acttgtactg gacaaggatt
      601 ccagaatctc aggatattat atccggacag tccctgcagc agagacagat tttccaaaaa
      661 tagaaagaaa catttccagg attcagtttc ctgccgaagg agtctggtcg acgacctggg
      721 gtggggatac aaaagaatta aattatcatg tagaaaaaaa attccagaag aatgcatttg
      781 attttgtcat cagggacgaa agaaacagct cttataaaaa cgacggaaaa aagaatgaag
      841 actattatgc attcgggaaa gaactttttg cacctgcaga cggaattgca gtgtatgtaa
      901 aggacggttt tcctgacaat aaaccgggag aaaaaacaga caagccgaat gaggtcataa
      961 tccggacaga gaatggagaa tatgttgtcc ttctccactt taaaaagaat tcaatagcag
     1021 tgaagcaggg ggaaaagatt aagaggggcc agcttctcgg tctggtcggg aattccggtt
     1081 tttcttccga gcctcatatt cattttcata tgatgaacgg ttttc
//
LOCUS       DAOQGJ010001783         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109843_length_1124_cov_3.866230, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001783 DAOQGJ010000000
VERSION     DAOQGJ010001783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109843_length_1124_cov_3.866230"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PK683_21495"
     CDS             <1..875
                     /locus_tag="PK683_21495"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNH11086.1"
                     /translation="ASDDRTLYFSSNGHNGLSVYVTQRVGESWTEWSDPVEISKNLKG
                     VNFFSIPASGNWAYMSRQGQLLMTFLPQEVKPDPVILVKGKVTDDKGEPVGAEIIYES
                     LTTKDRKGSTISDPNTGHYSIVLPYGDNYGFFAQKETYMPVHKNYDLSKIEKSSKEYK
                     EVIVDFTLPRVEEGQEFVINNLFFDYNKSDIKKESEAELDRLGKMMNSNKEIIVSLEG
                     HTDNIGRNEDNVALSLARAKSVAEYLQEKHSIPRERFKVSGYGEMRPLVPNDSPENRA
                     KNRRVVFKILKKNK"
BASE COUNT          356 a          223 c          267 g          278 t
ORIGIN      
        1 tcgcatccga cgacagaact ctgtattttt cgagcaatgg acataacggt ctgtctgttt
       61 atgtgactca gcgtgtaggg gaaagctgga cggaatggtc tgatcctgta gaaatttcca
      121 aaaacctgaa aggagtgaat ttcttttcca ttcctgcatc gggcaactgg gcatatatga
      181 gcagacaggg acagcttctg atgacgttcc ttcctcagga agtcaagcct gatcctgtca
      241 tcctggtaaa gggaaaagtg actgacgaca aaggagagcc tgtgggagca gagattattt
      301 atgaatcgct gacgacaaag gacagaaagg gcagcacaat ctctgatccg aacacaggac
      361 attattctat tgtacttccc tacggagaca attacggttt ttttgcgcag aaagaaactt
      421 atatgcctgt ccataaaaac tatgatctga gcaaaattga aaaaagcagc aaggaataca
      481 aggaagtcat cgttgacttc actcttccca gagtcgaaga aggccaggag tttgtaatca
      541 ataatctgtt ctttgattat aacaaatctg atattaaaaa ggaatcagaa gcagagctcg
      601 acagactggg caagatgatg aattccaaca aggaaatcat tgtttccctc gaagggcata
      661 cggacaatat aggccggaat gaagacaatg tggctctctc tctcgcacgt gcaaagtctg
      721 ttgcagagta tcttcaggag aaacacagca ttcctagaga aaggttcaaa gtttccgggt
      781 acggagaaat gagaccttta gttccgaatg actcacctga aaacagggca aaaaacagaa
      841 gggttgtttt caagattctg aagaagaaca aatagaatac cttgcacaga tggagcattt
      901 ttggcgggaa cccggcagct tgagggctcg cagggtatgg aagcggctgc ccctcgccaa
      961 aaagatggct taactcccgc aagcctggaa gatgctgttt ttactgattc atcgcaagct
     1021 gttaaactgc tggattttcc caatgttttc tgaaaatcca gcgtccagac aaagaaagat
     1081 gagtgagtat aacaggaata ataagctcag atttatattt tttt
//
LOCUS       DAOQGJ010001784         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109873_length_1124_cov_3.071094, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001784 DAOQGJ010000000
VERSION     DAOQGJ010001784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109873_length_1124_cov_3.071094"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>1124
                     /locus_tag="PK683_21500"
     CDS             76..>1124
                     /locus_tag="PK683_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11087.1"
                     /translation="MQKINLDTIFKNASDICRDIQENTENRKIIISLLLLIFVCTGLY
                     GLVMGFSHSWQQSLSSFLKVPLLYLLTLFVCIPTLHFAGLFLGSGISFFQSVSIMLLG
                     IATNAAFLLGFTSISIFFYVTGSNYKFLLLMHILFFTVGGIAGLISINRSYNFLSAEL
                     DGSLKKNRTLLLKIWMVLYMFVGTQMAYILSPFVGKDEIFHLFHHPKGNFYSYVTDLI
                     QDKFTDQEMDSKKAEDLVSEKARIAVNSLKEKNFVRLTSLIGDKGLKIILNSEPSRFP
                     GTEIDPVFSAASLLEAFEKNRIILIRDKNLSDSGNPVQELKIPFLQLYNEYLYDYNYA
                     DIQITPNFNKFTLGR"
BASE COUNT          312 a          220 c          221 g          371 t
ORIGIN      
        1 gaatgactga gtaaataaag actgtgcaat gttctgagta ttcaggctta gcacagtttc
       61 acagggaaag ctgctgtgca gaaaataaat cttgatacaa tctttaaaaa cgcttcagat
      121 atttgcaggg acatccagga aaatactgag aacagaaaaa ttataatttc gctgcttctg
      181 ctcatatttg tctgcactgg actttacgga cttgtaatgg gtttctctca ttcctggcag
      241 cagtctctgt cctctttttt gaaagtgcct ctgctctacc ttctgacatt atttgtctgt
      301 atcccgaccc tgcattttgc cggtctcttc cttggttcag gaatcagctt ttttcagtct
      361 gtttccatta tgctgctggg gattgcaacc aatgcagcct ttctgctcgg gttcacatcc
      421 atttccattt ttttctatgt aacaggttct aattataaat tccttcttct tatgcatatt
      481 ttatttttca cagtcggagg aatagcgggg ctgatttcca ttaacagaag ttataatttt
      541 ctctctgccg aactggacgg ttctctcaaa aagaacagga cgcttctttt gaaaatctgg
      601 atggttctgt atatgttcgt cggtactcag atggcatata tactttctcc ttttgtagga
      661 aaggatgaaa tatttcatct ttttcatcat cccaaaggaa atttttactc atatgtaact
      721 gatctgatcc aggacaaatt tacagatcag gaaatggatt caaaaaaagc ggaggatctg
      781 gtttctgaaa aggcgaggat agcggtaaat tctctgaaag aaaaaaactt tgtccgtctg
      841 acctcactca tcggtgataa aggtctgaag atcatattga attcagaacc gtcccgattt
      901 ccgggcactg agattgatcc ggttttttca gcagccagtc tgctggaagc gttcgagaaa
      961 aacaggatta tactgattag ggataagaat ctttctgatt cgggaaatcc tgtccaggaa
     1021 cttaaaattc cgttcctgca gctctataat gaatatttgt atgattataa ttatgcagat
     1081 attcagataa ctccaaactt taataaattc acactggggc gtga
//
LOCUS       DAOQGJ010001785         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_109905_length_1124_cov_2.672591, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001785 DAOQGJ010000000
VERSION     DAOQGJ010001785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109905_length_1124_cov_2.672591"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PK683_21505"
     CDS             complement(<1..1110)
                     /locus_tag="PK683_21505"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNH11088.1"
                     /translation="MEFPEHIKNIKKGIVHLREYSARDKESERRARAGILSMENSRQA
                     MEGEYAGISDPRVYSLLLKKEEEFQKLLSKQPEKAAIYREAYSKIEAAVEKKRERIHE
                     IMNSRLSGTLFHYALSIVRYLIEKEKPNEKRYEEYRDSSLDSFRHRIFSNAPVYKDKE
                     KFMLKKSMERLREELGENHRFVSQIFQDYERIEKITDSTKLDDPEFRKKVFEMKIDDL
                     DRTQDEFLLWVKKLEPYYRELREWHEKEIEEVFLNETEKIQRLKFELSGKETYPDATF
                     SLRLSYGKIKGMELDGFQITPFTTLHGLMERHENFKKNPEFVLPEKFLSAVEKLDLKK
                     KLNFISTHDIIGGNSGSPVVNLKGELVGLIFDGNAY"
BASE COUNT          281 a          263 c          185 g          395 t
ORIGIN      
        1 gtatgcattt ccgtcaaaga tcagacctac aagctctcct ttcagattca caaccggact
       61 tccggaattt cctccgataa tgtcgtgagt ggaaataaag ttcagttttt tcttcagatc
      121 cagcttttcc actgctgaga gaaatttttc gggaagtaca aattcaggat ttttcttgaa
      181 attttcatgt ctttccatta aaccgtgcag agttgtaaaa ggagtaatct gaaaaccgtc
      241 cagttccata cccttgatct tcccgtaact cagacggaga gaaaatgttg catccggata
      301 agtctccttt ccggaaagtt caaacttcag tctctggatt ttctcagtct cattcaggaa
      361 aacttcctca atttcctttt catgccattc cctaagttcc ctgtaatacg gctcgagctt
      421 tttaacccag agaagaaatt catcctgagt cctgtccaga tcatcaatct tcatttcaaa
      481 gacttttttt ctgaattcag gatcgtccag ttttgtggaa tcggtaattt tttcaatcct
      541 ttcatagtcc tgaaaaatct gagagacaaa tctgtgattt tctccaagtt cctccctcag
      601 acgctccatg cttttcttca gcatgaattt ttccttatcc ttatatacag gagcattaga
      661 aaatattcta tgcctgaatg aatccagaga tgaatccctg tattcttcat agcgtttttc
      721 gttcggtttt tctttttcaa tcagatagcg gacaatgctc aatgcataat gaaaaagtgt
      781 accggaaagt cttgaattca ttatttcatg aatacgttct ctcttttttt ccaccgcagc
      841 ttcaatcttt gaataggctt ctctgtaaat cgccgccttt tcgggctgct tcgaaagaag
      901 tttctgaaac tcttcttctt ttttcagaag aagggaatag actctcggat cgctgatccc
      961 tgcgtattct ccttccatcg cctgtctgga attttccatg ctcagaattc cggcccttgc
     1021 cctcctctct gactccttgt ctctggctga atattctctc agatggacta tgcctttttt
     1081 tatatttttg atatgttcag gaaattccac atccttcata aatt
//
LOCUS       DAOQGJ010001786         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_110095_length_1123_cov_2.597378, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001786 DAOQGJ010000000
VERSION     DAOQGJ010001786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110095_length_1123_cov_2.597378"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PK683_21510"
     CDS             <1..542
                     /locus_tag="PK683_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11089.1"
                     /translation="YGIRLSQNFCKSKLEENIAYRRTESLEDTVIRDMTVQEYEADYS
                     SRLFKDEKTRFELGRHLHVHTVKTHLADDRFVPHSEWKYMEKSAEALSRAGIKFIIVN
                     NPESPDELKIYKDGKWYKGYLDYMNSLTKIKGVYFFDRKDFIKDLRRFWDTHHLTTVG
                     ADEMTEEYAKIITDVYNEK"
     gene            532..>1123
                     /locus_tag="PK683_21515"
     CDS             532..>1123
                     /locus_tag="PK683_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11090.1"
                     /translation="MKNKYFPFLAFLVLYAAVTFSVWKKYEWNPASMIHFGAEFAVKN
                     ASRMPENSIVERGSAGDLGAGYDGQIFYFYSRTISELSWDWPKGFDESYRAPRIGYPF
                     LIGLFGFAGKKAAVFGMYFWNISLFILSVFALRSMLNEKHRYLSWFYILSPFSLGSYS
                     VLVSDSVMTSLVIIAYYFFVTGRNIPFILTASLSILT"
BASE COUNT          355 a          217 c          245 g          306 t
ORIGIN      
        1 tttacggaat cagactgtct caaaatttct gcaaatcaaa actcgaagaa aatatagcct
       61 acagaagaac tgaatcactg gaagacactg tgatcagaga tatgacagtt caggaatatg
      121 aggcagacta cagcagtaga ctcttcaagg atgaaaagac aagatttgaa cttggaagac
      181 atcttcacgt gcacacggta aagactcatc ttgcggatga cagatttgtc cctcattcag
      241 agtggaagta tatggaaaaa tcagcagaag cactcagcag agcggggata aaattcatta
      301 tagtaaacaa tccagaaagt ccggatgaac tgaaaatcta taaagacggg aaatggtaca
      361 aaggatatct ggactatatg aacagtctga caaaaatcaa aggggtctat ttctttgaca
      421 ggaaggactt catcaaggat cttcggagat tctgggacac gcatcatctt actacagtag
      481 gcgccgatga gatgacagaa gagtatgcaa aaatcattac ggacgtttat aatgaaaaat
      541 aaatatttcc catttctggc attcctcgtc ctttacgccg ctgtcacttt ttctgtctgg
      601 aagaaatatg aatggaatcc agcatcaatg attcatttcg gagcagagtt cgcagtaaag
      661 aatgcttcaa ggatgcctga aaattccatc gtggaaaggg gcagtgcagg cgatctggga
      721 gcaggctacg acggacagat attctacttt tattccagaa caatcagcga gctttcctgg
      781 gactggccta aaggatttga tgagagctac agagcaccca ggatagggta tccctttctg
      841 ataggactgt tcggatttgc cggaaaaaag gcagcggtat tcggaatgta tttctggaac
      901 atatctcttt ttatactgtc agtatttgca ctcagaagca tgctgaatga aaaacacaga
      961 tacctgtcct ggttttacat actgtctcct ttttcccttg gaagctactc tgtgcttgtc
     1021 agtgattcag taatgacatc tcttgtaatt atagcatatt atttttttgt cacgggaagg
     1081 aatattcctt tcattctgac tgcatcactt tccattctga cca
//
LOCUS       DAOQGJ010001787         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_110332_length_1122_cov_2.056232, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001787 DAOQGJ010000000
VERSION     DAOQGJ010001787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110332_length_1122_cov_2.056232"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PK683_21520"
     CDS             complement(<1..347)
                     /locus_tag="PK683_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11091.1"
                     /translation="MKPKKYLISLLILTAGLNIADCKKKRLENPPVTRKCEETTGGNL
                     YGKLGDCLKDKKECIPGVCVEGDCENGKGEMRFASGGFYIGDFKNGEFEGQGTLTYCT
                     EGYYTGGFKQSLS"
     gene            763..>1122
                     /locus_tag="PK683_21525"
     CDS             763..>1122
                     /locus_tag="PK683_21525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11092.1"
                     /translation="MKNKKDTEKNGRSRLISESSVKQAEKMKPLITEKRSKLDPDGYT
                     VHNEFEIEITKTNLVILVNTGWNCGWTVKKAPKKSEFQSGDRLCASDWMVDNYGPEGI
                     ALKYNDKEFVVNWHDQPE"
BASE COUNT          346 a          213 c          218 g          345 t
ORIGIN      
        1 tatgaaagac tttgtttaaa acctcctgta tagtaacctt cagtacagta ggttaatgtt
       61 ccctgtcctt caaactctcc attcttaaaa tcacctatat aaaaaccgcc gctggcaaat
      121 ctcatctcgc cttttccgtt ttcgcagtct ccctctacac ataccccagg gatgcactct
      181 tttttatcct ttaagcagtc gcctaatttt ccatagagat ttccaccggt cgtttcctcg
      241 cattttcttg tgacaggcgg attctccagc ctctttttct tgcaatctgc aatgtttaat
      301 cccgctgtca gaatcagtaa agaaatgaga tattttttgg gtttcattta aaccctcctt
      361 tggcaattag aaatggtcat agtcagtgaa gcacagttac tgaacgactt cacatttgtg
      421 catcctatct catgttcaat aatttgtcga ctggaaaatg aggagctcct tataggagaa
      481 tcattttatt ataaaatcat tcttttctct cttcagtctc ctgtaagttc tttaaatttt
      541 tgagggaagc agaatatttt cctgttgatt tattttgagt ttttgttttt ggtttcttca
      601 aataaagttt ttatggttct tgatcggaga gtccatttta ttttaaatat gaatgagatc
      661 aaagaatcgg ttttctgaaa gtttagacag aagtctggaa ttgatatagg cagttttgtc
      721 tgcagaacgg aacagttgaa acaggaaaaa ggagaatatc gaatgaagaa caaaaaagac
      781 actgagaaaa atggccgaag caggctgatc agcgaatctt ctgtcaaaca ggccgagaaa
      841 atgaagcctc tcataaccga aaaaagaagt aaacttgacc ctgacggcta cacagttcat
      901 aatgagtttg aaatagagat taccaaaacg aatctggtga ttctggtaaa cacaggatgg
      961 aactgcgggt ggacagttaa aaaagcaccg aaaaaatctg aattccagtc gggagaccgt
     1021 ttatgtgcaa gcgactggat ggtagacaac tacggtccgg aaggaatagc attaaaatat
     1081 aatgataaag aatttgtagt caactggcac gatcagccgg aa
//
LOCUS       DAOQGJ010001788         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_110439_length_1121_cov_3.151970, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001788 DAOQGJ010000000
VERSION     DAOQGJ010001788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110439_length_1121_cov_3.151970"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK683_21530"
     CDS             <1..212
                     /locus_tag="PK683_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11093.1"
                     /translation="VPLPAQDIGEICSAEKLCLNSICLDGKCHEKGSEDRIPWKCNEK
                     GCGVSGASCRLDSECKGICVNGKCQ"
     gene            332..643
                     /locus_tag="PK683_21535"
     CDS             332..643
                     /locus_tag="PK683_21535"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNH11094.1"
                     /translation="MNAREKEILHTLSEKIKAIFPDTKIWLFGSRAKETAEPDTDYDI
                     FVVCRDAVQRKQISEILWETGFENDMLLVGSYIEEILFSDPKYRQSMFIQSAMKDCIS
                     L"
     gene            640..1071
                     /locus_tag="PK683_21540"
     CDS             640..1071
                     /locus_tag="PK683_21540"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNH11095.1"
                     /translation="MNIDEKKDFVIFRMKEAKESYLDSILLLENKSFRSSVNRAYYSM
                     FYAVLALANLSELSVSKHAGVIAFFDKEYIKTGIFTKEYSKYLHTAFELRQESDYLDF
                     KDISLNDAEKAVKAAEKFLKTVFQHIQEKYSLFFPFSENDQ"
BASE COUNT          371 a          189 c          220 g          341 t
ORIGIN      
        1 gagttcctct tcctgctcag gatatcggtg aaatttgttc tgcggaaaaa ctctgcctga
       61 acagcatctg tctggatgga aaatgccatg aaaaaggttc tgaagacaga atcccttgga
      121 aatgtaatga aaaaggatgc ggagtctcag gagcttcctg cagactggat tcagaatgca
      181 agggtatctg tgtcaacgga aaatgtcagt gaatctcaaa tcgcaatcat gataaaaata
      241 tttcgagact tcgcagagtc aatctgtacc tgactgaaaa ggatttgaca tattccggaa
      301 atctttcaag atagccttgg agctgtcata gatgaacgct agagaaaaag aaattctgca
      361 tactctgtcc gagaaaatca aggcaatatt tccagatacg aaaatttggc ttttcggttc
      421 aagggccaaa gaaacggcag agccggatac agattatgat atattcgttg tctgcaggga
      481 tgccgttcaa agaaaacaaa tcagtgaaat tttatgggaa actggttttg aaaacgatat
      541 gcttttagtc ggctcttata ttgaagaaat ccttttttca gatcctaaat atcggcagag
      601 tatgtttatt caaagtgcta tgaaggattg tatttcttta tgaatattga tgaaaaaaaa
      661 gattttgtaa ttttccgaat gaaagaagct aaggaatctt atctggattc cattcttttg
      721 ctggagaata aaagtttcag atcttcagtg aacagagcat actattctat gttttatgca
      781 gttcttgcat tggcaaatct gtcagaactg tcagtttcca agcatgctgg agtaatcgct
      841 ttctttgaca aggaatatat aaaaacagga atttttacta aagaatattc aaaatatctc
      901 catactgcat ttgaacttcg tcaagaatca gattatctgg atttcaagga tatctcactt
      961 aatgatgcag aaaaagcggt aaaggctgca gaaaagtttc tcaaaacagt atttcagcat
     1021 atacaggaaa aatacagtct cttctttcct ttttcagaga atgatcaatg aaacatatca
     1081 tcattatatt tctattattc ttatcccact gccagtctgt c
//
LOCUS       DAOQGJ010001789         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_110602_length_1120_cov_4.615962, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001789 DAOQGJ010000000
VERSION     DAOQGJ010001789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110602_length_1120_cov_4.615962"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1047)
                     /gene="dcm"
                     /locus_tag="PK683_21545"
     CDS             complement(133..1047)
                     /gene="dcm"
                     /locus_tag="PK683_21545"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNH11096.1"
                     /translation="MNILSLFDGMSCGQIALNKAEIPYSKYYASEIDKYAIQITQKNY
                     PETVQLGDVSKINIDSLPKIDLLIGGSPCQGFSAAGKKLNFEDPRSKLFWEYVRIKEE
                     LNPKWFLLENVMMKKEWIDIISSALKVQSIMINSSDFSAQNRKRLYWTNIQVRKWGKK
                     EIYLKDILESVTSDRLKSYCIDAQYYKGITLQSYITKHRRTQIFETVRLGEICKGGQG
                     QRIYSVNGKSISLSTQSGGQGAKTGLYQIDLPDGQYYIRKLTPIECERLQTVPDNYTE
                     GVSNTQRYKMLGNGWTVDVIAHILSGMK"
BASE COUNT          289 a          238 c          187 g          406 t
ORIGIN      
        1 ttcatttcat cccttccttt aggacataca ggcctagctc tggattaaca cagttcctta
       61 agacctgcat cttgtccttg atatgagtat cggcaatgtc gaacaaatct ttatagtcgc
      121 ttattttatt tttcacttca tcccactcag tatatgagca attacgtcca ctgtccagcc
      181 attgcctagc atcttgtatc gttgagtatt tgacactccc tcggtgtaat tatcaggaac
      241 agtctgaagt ctttcacatt caataggagt aagttttcgt atataatact gtccatctgg
      301 tagatcaatt tgataaagtc ctgtctttgc accctgtcct ccgctctgag tacttagaga
      361 aattgacttc ccgtttacag aataaattct ttgtccctgt ccgcctttgc atatttcgcc
      421 aagtcttaca gtttcaaata tctgagttct tctgtgtttt gtaatgtaag attgtaaagt
      481 tattcctttg taatattgag catctatgca ataagatttc agtctgtctg acgtcacgct
      541 ttccagaatg tctttcagat atatttcttt cttcccccat ttccttactt ggatatttgt
      601 ccagtaaagt cttttcctgt tctgcgcaga aaagtctgaa gagtttatca ttatcgactg
      661 aacctttaaa gcactgctga taatatcaat ccactctttt ttcatcatta cattttccag
      721 caaaaaccat tttgggttaa gttcttcttt gattctgaca tattcccaaa ataacttact
      781 tcttggatct tcaaagttaa gtttcttccc ggcagcggaa aacccctgac agggagaccc
      841 gccgataaga aggtcaatct ttggcagtga gtcaatattg atttttgaaa cgtctcctag
      901 ttgaacggtt tcaggatagt ttttctgagt aatttgaatt gcatatttat ctatttcaga
      961 agcatagtat tttgagtatg ggatttctgc tttatttaga gcaatctgtc cgcagctcat
     1021 accgtcaaag agtgataata tattcatagt ttacggttcc cgcctagacc ggaatacagg
     1081 ttaagtattt tcaatctgca tacctttcag cgtaactgac
//
LOCUS       DAOQGJ010001790         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_110671_length_1120_cov_2.508920, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001790 DAOQGJ010000000
VERSION     DAOQGJ010001790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110671_length_1120_cov_2.508920"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK683_21550"
     CDS             <1..695
                     /locus_tag="PK683_21550"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNH11097.1"
                     /translation="RAGGGISEKREIPSEQHIPEGVELSQVMFVNPQSLYPNPLNKFE
                     LLKDKEYSDLKADIEKRGIIVPLIAKPDGILLAGHNRLKIAAELGVQKIPVQYVADFL
                     SRETEIEFIYKDNVLRRQLSNAERLNVYRAYFPDFDEAVEKENRGGKRGKSISDTFDG
                     KITAKIISQKTGIPEITVKRDLNKARTVKKGVNSDILKLKEKKKKIIAQIRQLQKELG
                     RIEKEILKAGSK"
     gene            692..>1120
                     /locus_tag="PK683_21555"
     CDS             692..>1120
                     /locus_tag="PK683_21555"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNH11098.1"
                     /translation="MKKQFVLLNFGAPRTQEEIGKFLKDLFGDKYIFDLPLPEFFRKR
                     LGRFIAERRVSKVKASYAAMGFGGGSPLYDNTLEQAKAVQAYLKARTGEDWDYKVGMS
                     CGEPNLSGFDPGDLVPNSGNVIVPLFPQYSRSTVLSMNRQI"
BASE COUNT          344 a          212 c          297 g          267 t
ORIGIN      
        1 ccagagccgg aggcggcatt tccgagaaaa gagagattcc ttccgagcag catattccgg
       61 aaggagtgga actgtctcag gtgatgtttg taaatcctca gtcactgtat ccgaatccgc
      121 tgaacaagtt tgaacttttg aaggacaagg aatactccga cctcaaggcc gacatagaga
      181 aacgcggaat aatagtccct ttgattgcaa agccggacgg aattcttctg gcaggtcata
      241 acaggctgaa aattgcggcg gaacttggag tgcagaagat accggttcag tatgtggcag
      301 acttcctttc cagggaaacc gagatagagt ttatctacaa ggataatgtt ctcaggagac
      361 agctttcgaa tgctgaacgt ctgaatgttt atcgcgcata tttcccggat tttgatgaag
      421 cagtggaaaa ggaaaaccga ggaggcaaga gaggcaaaag tatcagtgat acttttgatg
      481 gaaaaataac tgcaaagatc atttctcaga agacgggaat tcctgagatt actgtaaaga
      541 gagatctgaa caaggcaagg acggtaaaga agggtgttaa cagcgatatt cttaagctga
      601 aggagaaaaa gaagaagatc attgctcaaa taaggcagct tcagaaagaa ctcggcagaa
      661 ttgagaaaga aattctgaag gcaggttcaa aatgaagaag cagtttgttc tcctgaattt
      721 cggggcaccg aggacgcagg aagaaatcgg caagtttctg aaggatctct ttggggataa
      781 gtatattttt gatctccccc ttcctgaatt tttcagaaaa aggctcggaa ggtttatagc
      841 agaaagaaga gtttcgaagg tgaaggcatc gtatgcggca atgggctttg gtggcggatc
      901 tcctctctat gacaatacct tggagcaggc aaaagcagtt caggcttatc tgaaagctag
      961 aaccggagag gattgggatt ataaagtggg aatgtcctgc ggagagccga atctgtctgg
     1021 ctttgatcct ggcgacctgg tcccgaattc cggcaatgtc atagtccccc ttttcccgca
     1081 atattcaaga tccactgtcc tcagtatgaa tcggcagata
//
LOCUS       DAOQGJ010001791         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_110713_length_1120_cov_2.065728, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001791 DAOQGJ010000000
VERSION     DAOQGJ010001791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110713_length_1120_cov_2.065728"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PK683_21560"
     CDS             complement(<1..332)
                     /locus_tag="PK683_21560"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNH11099.1"
                     /translation="MKKLINLFLLSVSLFSLRLSAVPEFSEVKESFISSESSVLDIDG
                     FVIQEGRTNTKERRLNWITLSEISYHLVKTAVIAEDKRFYEHSGVDWTAFASGTVKFF
                     IGESRGAST"
     gene            437..>1120
                     /locus_tag="PK683_21565"
     CDS             437..>1120
                     /locus_tag="PK683_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11100.1"
                     /translation="MGTFQKFKIFQSSTFYFFILLLISFILYWEPAYRAPILNFDDRQ
                     LLIPMANVRSIYDYISLWQGNMIMDLQPVRDLSYFLEYRLAAVQGIGIHPQAFNVLLW
                     ISACFFAVKIFRMQGFADLESKLAGLFLSVHPVAVNSIAWSSGRKHVLSLFFISIASW
                     LWLRWLHSEKRGVPFSAVLFYILSCLSQPINAGWVLWAGLTVYLFHDSVSQRNEKIRN
                     SAVPIILCLI"
BASE COUNT          295 a          248 c          217 g          360 t
ORIGIN      
        1 gtgcttgctc ccctgctttc cccaataaaa aattttactg tacccgatgc aaaggcagtc
       61 cagtccacac cgctgtgttc ataaaatctt ttatcttctg caatcacagc agtcttgact
      121 aaatgatagg aaatttccga gagagtaatc cagttcagtc ggcgttcctt cgtattcgtt
      181 ctgccttcct gaatgacaaa tccgtcaatg tccagaacag acgactctga agaaataaaa
      241 gattctttga cttctgagaa ctccggcact gcagaaagac ggagagaaaa cagtgatact
      301 gaaagcagga ataaattaat cagttttttc ataatgaact gattccagaa tcttctatgg
      361 cattcagact gtcattctca atccaaaaac cttgacaggc ttgtctgcag aatcaaaatg
      421 aaatcaggag cagattttgg gaacatttca gaaatttaaa atttttcaga gcagcacatt
      481 ttattttttc attctgcttc ttatttcatt tattctttac tgggaaccgg cttacagggc
      541 tcccatcttg aacttcgatg acagacagct cctgattcca atggcaaatg tccgttcaat
      601 atatgattac atatctctct ggcagggaaa tatgattatg gatcttcagc cggtcaggga
      661 tctgagctat tttctggaat acagacttgc cgctgttcag ggcattggaa ttcatcctca
      721 ggctttcaac gttctgcttt ggataagtgc ctgttttttt gcagtaaaaa tcttccgaat
      781 gcaggggttt gctgatctgg aatcaaagct tgcaggactc tttctttccg ttcaccctgt
      841 cgcagtcaat tccatcgcct ggagttccgg aagaaagcat gtactctcct tgttttttat
      901 ttcaattgca tcctggctct ggcttcgctg gcttcattca gagaaaaggg gggttccgtt
      961 ttcagctgtt cttttctata ttctttcctg cctctcacag cccatcaatg caggatgggt
     1021 tctctgggca ggactcactg tatatttatt tcatgattct gtatcccaaa gaaatgagaa
     1081 aatcagaaat tcggcagttc cgataattct ctgtcttatt
//
LOCUS       DAOQGJ010001792         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_110983_length_1118_cov_3.450611, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001792 DAOQGJ010000000
VERSION     DAOQGJ010001792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110983_length_1118_cov_3.450611"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..661
                     /locus_tag="PK683_21570"
     CDS             167..661
                     /locus_tag="PK683_21570"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH11101.1"
                     /translation="MKEDILRIWDYNFWASERFTAKLQNFSEEEFCRDLGDGCGSVRD
                     KLGHILGADKVWLERIQGKVNPFLPASEIKDIRFYMDLSEKIQSDYKKILNELTEENL
                     QNRISYKNLKGLDFSTPLFEILLHVANHATYHRGQAASLIRRIKGSPPVTDMIEYFRS
                     KSAG"
     gene            complement(664..>1118)
                     /locus_tag="PK683_21575"
     CDS             complement(664..>1118)
                     /locus_tag="PK683_21575"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH11102.1"
                     /translation="NGKIFQLSSLKGKTVFLYFGYTYCPDACPMTLAKLNAVYGKLQD
                     RRDQLRIVFVSVDPKRDTPKRLKEYLEYYKDLSPIGLTGKEEEIQKVVKLFAGSYKKS
                     DSVKSEEYYLVDHSTYTYLIDSGGITRYLFKHDDSVDTIANLVKVFLK"
BASE COUNT          361 a          215 c          242 g          300 t
ORIGIN      
        1 ggattgaaga aggctgcagg actctgaatg aaaaagatct tttattaaaa tataatgaaa
       61 aatggaagaa tttagttcag cagtaaggaa aaaaccaagt atgcaggaat ttgcggaatg
      121 agattatgaa aaaagagaac agatcggcag aacacagagg agaacaatga aagaagacat
      181 cttgagaatt tgggactaca atttctgggc aagtgaacgg tttactgcaa aactgcagaa
      241 tttttcagaa gaggaattct gcagggactt aggagacggg tgcggatcag tgagagacaa
      301 actcggtcat atcctcggag cagacaaggt ctggctggaa agaatacagg gaaaagtgaa
      361 tccctttctg cctgcatcgg aaataaagga tatccgtttt tatatggatc tttccgaaaa
      421 aatacagtct gattataaaa aaatactgaa tgaactgacc gaggaaaacc tgcagaaccg
      481 gatttcctat aagaatctga aggggctgga tttcagcact cccctttttg agatacttct
      541 tcatgtcgca aaccatgcca cctatcacag aggacaggca gcatctctca tcagaagaat
      601 caagggaagt cctcctgtca cagatatgat tgaatatttc cggtcaaaat cagcaggtta
      661 ggattatttc aaaaagactt tcacaagatt cgcaatcgta tcaacggaat catcgtgttt
      721 gaaaagatac cgggtgattc cgcctgaatc aatcagataa gtgtaagtgg agtgatccac
      781 cagataatac tcttccgatt ttactgaatc agatttttta taggaacctg cgaagagttt
      841 tacgaccttc tgtatttctt cttcctttcc tgtaagccca atcggagaca gatccttgta
      901 atattccaga tactccttca gtcttttggg agtgtctctt ttcgggtcca cgctcacaaa
      961 tacaattcgg agctgatctc ttctgtcctg cagttttccg tacactgcat tcagttttgc
     1021 aagagtcatc ggacaggcat ccggacagta ggtatatccg aaatacagaa aaactgtttt
     1081 tcctttgaga gaactcagct gaaaaatttt cccgttct
//
LOCUS       DAOQGJ010001793         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_111181_length_1117_cov_2.787194, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001793 DAOQGJ010000000
VERSION     DAOQGJ010001793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111181_length_1117_cov_2.787194"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..919)
                     /locus_tag="PK683_21580"
     CDS             complement(353..919)
                     /locus_tag="PK683_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11103.1"
                     /translation="MKKISALLFSAAGFLAILLIPVLLYADSPPSWSEFSVFSKGKKY
                     RADVKSVSNEKNPRDRKWKLSVFDSGKKKPLWSSDFDFRFGYPGGILSADGKTFIMTE
                     VWYHSDSVLVDIYREGKKLDTDHLTGKSFQISQEYLKRTASHFLWFEENRHYRFEKRS
                     GRDFFVLIPFDGREHWIDLSTGKRENPE"
     gene            919..>1117
                     /locus_tag="PK683_21585"
     CDS             919..>1117
                     /locus_tag="PK683_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11104.1"
                     /translation="MREVSRINGLLPSCPTEISFSPSAFTSAFCPSLILGSSFSGIYI
                     LMKTLLKSAAIRIGAPGITTSP"
BASE COUNT          316 a          282 c          204 g          315 t
ORIGIN      
        1 accgattgca cagcttgcaa ccaccagaat cacccccagc cgggatgacc atctttcttt
       61 aggattggga ttactcattc tataagcttt aaactataaa ccgactgatt tgggcaacta
      121 aaaatcttta tgatgaaaaa taccgaaacc tttcgctccg gctttatcct gcctgaactg
      181 ctgctcaatc catccgctga cttttcaggg ccagattcac aaaacttccg acagtcataa
      241 cgataatgag atttatgaac aggtgcaccc ggaattcctc acggaacttg atttccgcag
      301 ccgcgcttca gtccaatgaa aaatataata tatccgtttt tcattaagac aatcattccg
      361 gattttctct ttttcctgtg gaaagatcga tccagtgctc tctcccgtca aaaggaatca
      421 gcacaaagaa gtctctgccg ctcctcttct caaatctgta atgccggttc tcttcaaacc
      481 agagaaaatg agatgctgtt cgtttcagat attcctgaga aatctgaaac gactttccgg
      541 tgagatgatc cgtatccagt ttctttcctt cccggtaaat atcgacaagc acagaatcag
      601 aatgatacca gacctcagtc attatgaatg tctttccatc cgctgagaga attccgccgg
      661 gataaccgaa tctgaagtca aaatctgaac tccagagagg cttctttttt cctgaatcaa
      721 agacggaaag cttccatttc ctgtctctgg gatttttttc atttgaaaca gacttcacat
      781 ctgctctgta ttttttcccc ttggaaaaaa cactgaattc cgaccaggac ggaggtgaat
      841 cggcataaag cagaacagga atcagaagaa ttgcaagaaa ccctgcagca gagaaaagca
      901 atgctgaaat ctttttcatt gagagaagtt tcccgcataa acggacttct tccgtcctgt
      961 ccaacggaga tttcattcag tccatccgct ttcacttcag ccttctgtcc aagtctgatc
     1021 ctgggcagtt ctttttccgg tatatatatt ttaatgaaaa ccctgctcaa gtcagctgca
     1081 atcagaatcg gtgcaccggg catcacaacc tcgcctt
//
LOCUS       DAOQGJ010001794         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_111346_length_1116_cov_3.687088, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001794 DAOQGJ010000000
VERSION     DAOQGJ010001794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111346_length_1116_cov_3.687088"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..>1116
                     /locus_tag="PK683_21590"
     CDS             173..>1116
                     /locus_tag="PK683_21590"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH11105.1"
                     /translation="MEPLFKIVNFAQSSYVILEGERKSPNFFIIKDGRVSIKRDFPVP
                     GEKPVETLGPGDFFGVVAAMSQYPQIESAVAVTNLTLIAVQHSRFGELIQKNAPLAMK
                     IIRSFSKKLREMDRGQSGTKSPSSGSGSPESLNILFSMAENYFSQGNTESAVYLYQSY
                     IKYLPQGQYVVQAKQKLKTLGRPIELPAVTGTNRNYSQGQMVFCEGEPGEDLFILQRG
                     KVKISKMINGNEVILNIMKPGDIFGEMALLDNKPRSASAVAMENVDILSINKQNFESM
                     TVNQPQLMSRIITLLSERVWNAYKKISNSLIEDMNGRMA"
BASE COUNT          361 a          220 c          259 g          276 t
ORIGIN      
        1 ctgtcctcct ggctacggtg aacctggtcc gggaaaagct gttttttccg taagcgcctt
       61 catggatgtc ataggataca aaggccgata gtcggcagaa aaaaaacaaa aaatgcacag
      121 catttgtatc cgaatataaa aaagtccgat aatttaggaa gagaggaata ctttggaacc
      181 gttatttaaa atcgtcaatt ttgcccagtc gtcttatgtg attctggagg gtgaaagaaa
      241 atcccctaat tttttcatca tcaaagatgg cagagtgagc attaaaaggg attttccggt
      301 tccaggcgaa aaaccggtcg aaacactcgg accgggagat ttctttggtg tagttgctgc
      361 gatgagccag tatcctcaga tcgaatccgc agttgcagtg acaaatctga ccttgattgc
      421 agttcagcac agccgatttg gagagctgat acagaaaaat gctcctctgg caatgaaaat
      481 catccgttcc ttttccaaaa aactaaggga gatggacagg gggcagtcag gaaccaagag
      541 tccttcctct ggaagcggat cacctgaatc gctgaatatt cttttcagta tggcagagaa
      601 ctatttcagt cagggaaata ccgaaagtgc agtttatctt tatcagagct atataaaata
      661 tcttccacag ggacagtatg tagttcaggc aaaacagaaa cttaagacac tgggaagacc
      721 gattgaactt cctgcagtga cagggacaaa cagaaattat tctcagggac agatggtgtt
      781 ctgtgaaggt gagcctggcg aggatctctt tattctgcag cgaggaaaag taaaaatctc
      841 caaaatgatc aacggaaatg aagtaattct gaatattatg aagcccgggg acattttcgg
      901 ggaaatggcc cttctcgaca ataagcccag atcagcttca gcagtcgcaa tggaaaatgt
      961 ggatattctc tcgatcaaca agcagaattt tgaatcaatg actgtcaatc agcctcagct
     1021 gatgagcaga atcatcacac ttctttcaga aagagtctgg aatgcataca aaaaaatctc
     1081 caacagtctg atagaagata tgaacggaag aatggc
//
LOCUS       DAOQGJ010001795         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_111360_length_1116_cov_3.266730, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001795 DAOQGJ010000000
VERSION     DAOQGJ010001795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111360_length_1116_cov_3.266730"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..878)
                     /locus_tag="PK683_21595"
     CDS             complement(36..878)
                     /locus_tag="PK683_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HNH11106.1"
                     /translation="MKTLEDFCIHILRSPSLADKLTAPPHDLLIQRPVSAVPEKPERE
                     NRISFSDKKSKIPRLEHLNIAVNRGISMHHFANHELQAVEIFAWAILKYSEIPDENRM
                     DLFRSLLEEQKHLKLYLERMNEFEIEFGERPLNYIFWKFTPLMKTFEKFCAVMSVSIE
                     GANLDYAMIYRKTFEGLGDIKSSEIMKIVYQDELKHVKRGYKVIEAGRNDQSDWEYYR
                     SLLEHPFTPRRAKGYFFIPFTRENVGFSGEFIRNLGEYRDEFSNRKKEIIPEEMRSWG
                     IYSG"
     gene            complement(875..>1116)
                     /locus_tag="PK683_21600"
     CDS             complement(875..>1116)
                     /locus_tag="PK683_21600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11107.1"
                     /translation="LDELSIIISVSGGSADILRIPTENTCSLIGGKYRGRLEFGSPAD
                     LLIFSENPAEKPENIFSLEKVFYRGREQRSSVRKK"
BASE COUNT          304 a          257 c          206 g          349 t
ORIGIN      
        1 cggataaacg gacagttccc tctcaaaaag agagttcatc ctgaatagat tccccacgac
       61 ctcatctcct caggaataat ttcctttttt ctgttggaaa attcatccct gtattcacca
      121 agatttcgga tgaactcccc gctgaaacct acattctccc ttgtaaaggg aataaaaaaa
      181 tatcccttcg ctcttctggg agtgaaagga tgctcaagaa gcgatctgta atactcccag
      241 tcggactgat cattccttcc tgcctcgatg accttatatc ctctttttac atgcttaagc
      301 tcatcctgat atacaatttt cattatttct gatgacttta tatcccccag tccttcaaaa
      361 gtcttcctgt aaatcattgc ataatccaga ttggctcctt ctatggaaac ggacatcact
      421 gcacagaact tttcaaatgt cttcatcagc ggagtaaatt tccagaaaat atagttcaga
      481 ggtctttcgc cgaactcaat ttcaaattca ttcatccttt ccagatacag tttcagatgt
      541 ttctgctctt cgagaagact tcggaaaaga tccatcctgt tttcatcagg aatctcggaa
      601 tacttgagga ttgcccaggc aaaaatttct actgcctgca gttcatgatt tgcaaaatga
      661 tgcattgaaa ttccgcgatt gactgcaata ttcagatgtt caagcctggg aatcttggat
      721 tttttatcgg aaaaagaaat tctgttctct cgttcaggct tttcaggcac tgcagaaacg
      781 ggcctttgaa tcagaagatc gtgaggaggg gcagtcagct tgtctgcgag actcggtgat
      841 ctcagtatat gaatgcagaa gtcttcgagt gtcttcattt ttttcgaaca gaacttctct
      901 gttctctgcc tctgtaaaat accttttcta atgaaaaaat attctcaggc ttctctgcag
      961 gattttcgct gaatatcagc agatctgcag gtgaaccgaa ttcaagtctg cctctgtatt
     1021 ttcctccgat gagactgcat gtattttcag tcggaattct aaggatgtcc gcagagcctc
     1081 cggaaacaga aataatgatg ctcagttcat cgagac
//
LOCUS       DAOQGJ010001796         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_111400_length_1116_cov_2.592837, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001796 DAOQGJ010000000
VERSION     DAOQGJ010001796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111400_length_1116_cov_2.592837"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..>1116
                     /locus_tag="PK683_21605"
     CDS             72..>1116
                     /locus_tag="PK683_21605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11108.1"
                     /translation="MHPLLLGDYSSLVLNEFVSEIDITVISLFYFTFSIFAVFVYLKE
                     RELKSFLYAAVLSILAGIAGIYITDFKKHIYDNPKLYTLIFAPCLYAFSPALQLYIIS
                     IFGKGWKSSFLVSFRIFIVILIFSPLVPLTGDEKYYYILELPYIVLSAVSLINILIHI
                     VWFIRQENVNAFYFGLGASALFGSLIKIYLAHMKLIEFQGNHFSMGFLGFFAGLVLAS
                     GEEYFQSRKKLKEYTENLENIIIEKTEEIEKKNAILMEDEKKVIQMEKENTIKQEREN
                     IFADIHDNLGGKLLDLSFQLKALQPNSVLSESSSILLQNRIQEVMKGLRSRLLAFEDI
                     KKFGLVQRGFLGVV"
BASE COUNT          338 a          209 c          212 g          357 t
ORIGIN      
        1 tcttttatat tagttacatc aaaacttttt aatatattag aatatatatt ttttatatga
       61 aaactgaaat catgcacccc ctgctgctgg gagactacag ttctcttgtc ctaaatgaat
      121 ttgtttctga aattgacatc acggtcatca gtctgttcta ttttacgttt tccatatttg
      181 cagtttttgt ctatcttaag gagagagaac tgaaaagttt cctttatgct gcagttctca
      241 gcattctggc aggaattgca ggaatctata tcacggattt caaaaagcat atttacgaca
      301 atccgaagct ttacactctg atattcgctc cctgtcttta tgcattctcg cctgcactgc
      361 agctgtatat aatatccata ttcggaaaag gctggaaatc cagttttctt gtctcattcc
      421 gcatctttat tgtcatcctg attttttctc ctcttgttcc tctgaccgga gatgaaaaat
      481 actactatat cctggaactg ccgtacattg ttctgtctgc agtctcactg ataaatatac
      541 tgatccatat agtatggttc atcaggcagg aaaacgtaaa tgcattctat ttcggtctcg
      601 gcgcatctgc tctattcggt tcactcatca agatttacct ggctcatatg aaactcattg
      661 agtttcaggg aaatcatttc agtatgggct ttctgggatt ttttgcagga ctcgtgcttg
      721 catcaggaga agagtatttt cagtccagaa aaaaactgaa ggaatataca gaaaatctgg
      781 aaaatatcat tattgaaaaa acagaagaga ttgagaaaaa aaatgcaata ctgatggagg
      841 atgaaaagaa agtcattcag atggagaagg aaaatacgat caaacaggaa agagaaaaca
      901 tttttgcgga cattcacgac aatctgggag ggaaacttct ggatctttcc tttcagctga
      961 aagccctgca gcctaattcc gttctttccg agtcctcgag cattcttctt cagaacagga
     1021 ttcaggaagt aatgaaagga ctcagaagca gacttctggc atttgaagac attaaaaaat
     1081 ttggtttggt acaaagagga ttcttaggag ttgtaa
//
LOCUS       DAOQGJ010001797         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_111505_length_1116_cov_1.493874, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001797 DAOQGJ010000000
VERSION     DAOQGJ010001797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111505_length_1116_cov_1.493874"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PK683_21610"
     CDS             <1..239
                     /locus_tag="PK683_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11109.1"
                     /translation="MYADLSLDHQILKKVIERMKDLPEKEQDILASFILEHLGGKEGT
                     AGMDDLHPEETHPWMLEEAHSEFESESTEDIGLS"
     gene            557..>1116
                     /locus_tag="PK683_21615"
     CDS             557..>1116
                     /locus_tag="PK683_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNH11110.1"
                     /translation="MDFQKYKEINDTRLNYREMEDATVVSSYRNVGCGDGYRVYLKIE
                     DDRITDASYTTTGCGFGITALAMMTEIVKNKTVEEAENLTEDDIEKVFSFPERRKNYP
                     ESAAEAMQKAVADYLNGTGIRPEDRVTRSVALEKLKINGHLRGENLSQVVLEGEDLHG
                     VDFTGANLQNAFLQNSNLEGAILEDA"
BASE COUNT          353 a          222 c          292 g          249 t
ORIGIN      
        1 gcatgtatgc ggacctgagc ctggatcatc agatactgaa aaaggtcatc gaaagaatga
       61 aagacctgcc tgaaaaggaa caggacattc tggcctcttt tatcctggag catctgggag
      121 ggaaagaagg aacggcaggt atggatgatc ttcatccgga agaaacgcat ccatggatgc
      181 ttgaagaagc ccattccgaa tttgaatccg agagcactga agatatcgga ctgagctgaa
      241 gggataacct tttatttacg catttatccg agaagcaatc ctgtgcagac gggatataac
      301 atattgattt gagactggaa acggtcaaat tgacctttct cggaaccatt gccggggcag
      361 tgcgggtgac acgccccgga aactccgtct ctctcaaaaa gcggtacgaa tcagaattgt
      421 catgaatttt tcacttgcta agagagagag ggtcaggaaa cttcaatatt ggaacggaat
      481 ctgctgtttt tcagtaaact gactttttta ggtcaatgtg ctgacggcag acagataaag
      541 aagtgctgga ggaactatgg attttcagaa atacaaagaa atcaacgata cccgactgaa
      601 ttacagagaa atggaagatg caactgtcgt ttccagctac aggaatgtcg gctgcggaga
      661 cgggtataga gtgtatctga aaatcgaaga tgacaggatc actgatgcca gctatacaac
      721 gaccggatgc ggattcggaa tcactgccct ggctatgatg acagagatcg taaaaaataa
      781 aactgtcgaa gaagcagaaa atctgactga agatgatata gaaaaagtgt tttcatttcc
      841 ggaaagaaga aagaattacc cggagtccgc cgctgaagcc atgcagaaag ccgttgccga
      901 ttatttaaat ggaacgggaa ttcgtccgga agaccgcgta acgcgatcgg tcgctcttga
      961 aaaactgaaa ataaacggac accttcgcgg tgagaatctg tcgcaggttg tgctggaagg
     1021 ggaagatctt cacggtgtgg attttaccgg agccaatctc caaaacgcgt ttctccagaa
     1081 cagcaatctg gaaggagcca tactggagga cgccag
//
LOCUS       DAOQGJ010001798         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_111558_length_1115_cov_3.100943, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001798 DAOQGJ010000000
VERSION     DAOQGJ010001798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111558_length_1115_cov_3.100943"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PK683_21620"
     CDS             <1..274
                     /locus_tag="PK683_21620"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNH11111.1"
                     /translation="NELLRTAKNAGIEVKLVWGKVYPKYAENFIKHSLKEKWEKRIIE
                     MSSQYGYKFYNMNEVSDCDVFYDASHQSSLCYARQLNYLVDESERR"
     gene            complement(260..784)
                     /locus_tag="PK683_21625"
     CDS             complement(260..784)
                     /locus_tag="PK683_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNH11112.1"
                     /translation="MKRKKMTAIRSYRKGFTLVEVAIALALAATAIVSSYRLISSGMI
                     MQRKAISLTNAVFLAKIKMAQIEASPKIETTSAKGDLPGYVGYRYEVNIKEEEMNLLK
                     MAEGGQKNKPPADLLGKDNDAKLNDLLKKRGQAQDSKTAGLIKVFKISVKIIYPAGTS
                     DAAFEAETFKSSTF"
     gene            complement(747..>1115)
                     /locus_tag="PK683_21630"
     CDS             complement(747..>1115)
                     /locus_tag="PK683_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11113.1"
                     /translation="HNEVVIMKVDIEKRTYSAYRVIRGPEGIVEKKIIESSFPSNSYV
                     RDAVDLRGIKTDAGILQIPYTYTGIGEDYSIHFGDDSGIKKTLLVYRYNGKSLIKNGE
                     VTRKSKGGDEKEKDDSDPLL"
BASE COUNT          278 a          243 c          227 g          367 t
ORIGIN      
        1 gaatgaactt cttcggactg ccaaaaatgc aggaatagaa gtcaagcttg tatggggcaa
       61 ggtatacccc aaatatgccg agaatttcat aaaacacagt ctgaaggaaa aatgggaaaa
      121 gaggatcatc gaaatgtctt ctcagtacgg ttataaattc tataatatga atgaggtttc
      181 agactgtgat gtgttttatg atgcatctca tcagtcctct ctgtgttatg caagacagct
      241 gaattatctg gtggatgaat cagaacgtag atgatttgaa tgtttccgcc tcaaaggccg
      301 catctgaagt tcctgcagga tagataatct tgacacttat tttaaaaact ttgatcagac
      361 cggccgtttt agaatcctga gcctgtcctc tttttttcag aagatcgttc agtttagcat
      421 cattgtcctt tcccaaaaga tctgcgggcg gcttgttttt ctgtccgcct tccgccatct
      481 tgagaagatt catttcctct tcctttatat tgacttcata tctgtatccg acgtatccag
      541 gaagatcgcc ttttgcagaa gtggtttcaa tttttggact ggcttctatc tgtgccattt
      601 tgatttttgc aaggaagact gcatttgtaa gactgattgc ctttctctgc ataatcattc
      661 cgctgctgat cagcctgtaa gaggagacaa ttgcagttgc agcaagggcc agagcgatgg
      721 caacttcaac cagagtgaat ccttttctat aggagcggat cgctgtcatc tttttccttt
      781 tcatctccgc ccttcgattt cctggttact tcaccgtttt tgatcagtga ttttccgttg
      841 tacctgtaga ccagaagagt cttcttaatc cctgaatcat ctccgaaatg aatgctgtaa
      901 tcctctccga ttcctgtata cgtgtagggg atctgcagga ttcctgcatc tgtcttgatt
      961 cctctcagat caactgcatc cctgacataa ctgttggacg ggaaactcga ttctatgatt
     1021 tttttttcga ctatgccctc aggtccgcga atgacacggt aggcggaata agttcttttt
     1081 tctatatcca ccttcataat gacaacttca ttatg
//
LOCUS       DAOQGJ010001799         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_111739_length_1114_cov_2.870633, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001799 DAOQGJ010000000
VERSION     DAOQGJ010001799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111739_length_1114_cov_2.870633"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..532)
                     /locus_tag="PK683_21635"
     CDS             complement(98..532)
                     /locus_tag="PK683_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11114.1"
                     /translation="MLARWAGEPENTESGSKDFFYSAFKGAVKNTRNISPERLPEICI
                     RSAQETMQSEIFERILTDIEQNSRHGHEIVYEEGNKNLILKQKLGKEGKEVHLKNCRG
                     SAKAVSEKKFSEWKTCECEGYVRIRGGKDRIFSLFRHPQETE"
     gene            829..>1114
                     /locus_tag="PK683_21640"
     CDS             829..>1114
                     /locus_tag="PK683_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11115.1"
                     /translation="MENPQTEKLSDEEESSAFNSPAPAQNSDSKDKSKFSIRDSMYTE
                     NSVSKTRLLEYRISLNVEVDELSSARQQFLSLLSENKSFLTQAQTYPVSGK"
BASE COUNT          299 a          251 c          213 g          351 t
ORIGIN      
        1 cgaaccggtt tcttcaaatg aaacagaaga aggccgttca aagaacagaa gggtggaatt
       61 caagcttctg aaggcagagt aaacctcttt tctttattca ttcagtctct tgcgggtggc
      121 ggaagagact gaatatcctg tccttgccgc ccctgattct cacatagcct tcacattcac
      181 aggttttcca ttcactgaac tttttttctg aaacagcctt tgcactcccc ctgcagtttt
      241 tcaaatgaac ttcctttcct tcctttccca gtttctgctt caaaatgaga tttttatttc
      301 cttcttcata aacgatctcg tgcccatgcc tgctgttctg ttctatgtct gtcagtatcc
      361 tttcgaatat ttcggactgc attgtttcct gagcagatct tatgcagatt tcgggaagcc
      421 tttcgggaga aatgtttctc gtgtttttta ctgcaccttt aaatgcagaa taaaagaaat
      481 ctttggaacc tgactcggtg ttttcaggct ctcctgccca tcttgccagc atcctgtttt
      541 cttttttttg ttccgtagag cagaatgtgc agatcaagga agataacagt aagaccgttt
      601 tttttatgaa cttcatctgt tctaattttg cctgcagtat tctgcagtca attcggaagg
      661 tggttaattt tagaatgaca gaccgcaaaa aagaattatt tttgctctat gaaaaatcta
      721 ctgatataca ctctaattct acttatcttt tcagcctgct caaaaaagga cacggccccg
      781 aagacctcga ctaccgtcag tcagtcagga gcggcagaaa gaaaaaaaat ggaaaatccg
      841 cagacggaaa agctttctga tgaagaggaa agttctgcct ttaattcccc ggctcctgcg
      901 cagaattcag attcaaagga taagtcaaag ttttccattc gcgactctat gtacactgaa
      961 aacagtgtgt caaagacaag gcttttggaa tatagaattt ccctgaacgt tgaggtagat
     1021 gaactttcat ctgccagaca gcagttttta tccttactct cggaaaacaa gtcgttcctt
     1081 acacaggctc agacctatcc tgtttcagga aagg
//
LOCUS       DAOQGJ010001800         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_111839_length_1114_cov_1.787535, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001800 DAOQGJ010000000
VERSION     DAOQGJ010001800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111839_length_1114_cov_1.787535"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..>1114)
                     /locus_tag="PK683_21645"
     CDS             complement(227..>1114)
                     /locus_tag="PK683_21645"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNH11116.1"
                     /translation="PSAPNDCRAGTVLKNTGRLSSETPRILNGFILHISLPALILLHI
                     HSVKWEISSLIPMSMAWVQFGAGFLFFTVLSHILKLSVRSRICLILCGSLGNTSFLGI
                     PMIEMHFGSYAVPVGILCDQPGTFLVLSLLGLPLVLSAETGLNIKHLIIKTLKFAPFQ
                     AFILAVLLYPVQYPEFLNSVLKRLGDTLVPLALFSVGFQFEMKSFGRKKDLIFYGLFF
                     KLILSPFLFWIFYSVLLNMKSLYVQITVFESAMPAMITAGTLAMEHDLDPDLAASLVA
                     YGIIISSFTLYFWKTVLIQ"
BASE COUNT          355 a          224 c          270 g          265 t
ORIGIN      
        1 gacaagcagt gtatttccgc tgctgatgct tctcagaatc gtttttgtct gtctgtgttc
       61 aaatataaat acgggttctg tgctcatttc tgcttttcct cagagagtca attttcagaa
      121 tgatattata taaacaacaa ataaatcttc agtctgtatt tcgggatgaa ggaagaattc
      181 ctgcagggaa ggcagagtca agttcggcta atgccgtata tggaactcac tgaatcagaa
      241 ctgtcttcca gaaatacagt gtgaatgagg atataatgat tccgtatgcc acaagagatg
      301 ccgccagatc cgggtccagg tcatgctcca tcgcaagggt tcctgcagta atcattgcag
      361 gcattgcaga ttcaaagact gtaatctgaa cataaagact cttcatgttc agaaggacgg
      421 aataaaaaat ccagaaaaga aaaggagaga gaatcagctt gaaaaaaagg ccgtaaaaaa
      481 taagatcctt ctttctgccg aaggacttca tttcgaactg gaatcccacg ctgaaaagtg
      541 ccagggggac aagagtgtct ccgagtcttt tcagcacaga attcagaaat tcaggatact
      601 gcacagggta taaaagcact gcaagaataa atgcctgaaa gggagcaaac ttaagtgtct
      661 ttattatcag atgctttata ttcagtccgg tttctgcact cagcacaaga ggcagaccga
      721 gaagagacag gacaagaaat gtgcctggct gatcgcagag aattccgaca ggaactgcgt
      781 aagatccgaa atgcatttca atcatgggga tacccagaaa ggaagtgttt cccagagatc
      841 cgcagagaat caggcagatt ctgcttctga cggaaagttt cagaatatgc gaaagaacag
      901 taaaaaaaag aaagcccgcc ccgaactgca cccaggccat gctcatagga atcaaagagg
      961 aaatctccca tttcacagaa tgaatatgca gaaggatgag tgcagggagt gatatgtgca
     1021 gaatgaatcc gttcagaatg cggggagtct cggaggagag ccttcctgtg tttttcagaa
     1081 ccgttccggc ccggcagtcg tttggcgctg atgg
//
LOCUS       DAOQGJ010001801         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_112316_length_1111_cov_3.020833, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001801 DAOQGJ010000000
VERSION     DAOQGJ010001801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112316_length_1111_cov_3.020833"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PK683_21650"
     CDS             complement(<1..1080)
                     /locus_tag="PK683_21650"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNH11117.1"
                     /translation="MHDSAVVIVSESGKLLFASSLERVTRIKQDGRKPSLLFSGLNLD
                     KIKKIAVSTNYKLPDEEQKLSKVHPVPMKEASPWYLIHPQEFHTFFESLPAEKVYFCH
                     QLSHAHSAFWLSGFDDALCLTYDGGMANSHYFGGVYSGSRANKQIQILDQFSGTRYAK
                     VTSLYTIVTAMLGFTPNKHEGKITGLAAYGTENSECESALLEMFHDRYKEMEDVVQWI
                     DLYSDEFSPFLHVDPYKRKKLWSPLSGFSKEDLAHSVQKIAEEHILEILKNIKKYGWY
                     QDSICLAGGLFSNVRINQKVKEFGFKNIFICPPMTDDGTALGAALAAVSAEKEFAPEK
                     VRNMFLGYSYSDEEINFMSCDKCHLI"
BASE COUNT          326 a          237 c          194 g          354 t
ORIGIN      
        1 gatgaggtga catttgtcac aactcatgaa attgatttct tcatcagaat aactgtaccc
       61 taaaaacata ttcctgactt tttccggagc aaattctttt tcagcagaca ccgctgccag
      121 agccgcaccc aatgctgttc cgtcatcagt cataggtgga catataaata tattcttgaa
      181 cccgaattcc ttcactttct gatttattct gacgttggaa aacaaacctc ctgcaaggca
      241 gatcgaatct tgataccatc catacttttt aatgtttttc agtatttcca aaatgtgttc
      301 ttccgctatc ttctgaactg aatgagctaa gtcttctttt gaaaatcctg aaaggggtga
      361 ccagagtttc tttcgtttat atggatcgac atggagaaac ggtgaaaact catcagaata
      421 caaatcgatc cattgcacaa catcttccat ttctttataa cgatcatgaa acatttccaa
      481 taatgctgat tcacattcag aattttcagt tccatacgca gcaagaccag taattttgcc
      541 ttcatgttta tttggtgtaa atccaagcat tgctgttaca atcgtataca gtgaagtaac
      601 ttttgcatat cttgttccgg aaaactgatc tagaatctga atctgcttat tggcacggct
      661 tccggaataa actccgccga agtaatgcga gtttgccatt cctccatcat aagtcagaca
      721 taaggcgtca tcaaaaccac tgagccaaaa tgcagaatgt gcatgtgaaa gctggtgaca
      781 gaaatagact ttctctgcag gcaaactttc aaagaaagta tgaaattcct gagggtgtat
      841 gagataccat gggcttgctt ctttcattgg aacaggatgc actttgctca atttctgctc
      901 ttcatctgga agcttataat tggttgatac tgcaattttt ttaattttat ccaagttcag
      961 cccggaaaac agcaagcttg gttttcttcc atcctgcttt attcgggtaa ctctttccaa
     1021 agacgatgca aacagaagtt ttcctgattc agatacaatg actacagcag aatcatgcat
     1081 tccgctgtag tttattccaa taactgaatt t
//
LOCUS       DAOQGJ010001802         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_112678_length_1109_cov_3.013283, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001802 DAOQGJ010000000
VERSION     DAOQGJ010001802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112678_length_1109_cov_3.013283"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..611
                     /locus_tag="PK683_21655"
     CDS             57..611
                     /locus_tag="PK683_21655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH11118.1"
                     /translation="MLEIGSAAGFFLSEAKKKDLIAKGLEISNTEAEYAKRMGLDVDC
                     VPFEDFNTEKKFDIICAFFVLEHFPDQEASWRKIFSLLQPGGFLFLALPSLNGPTFRT
                     DSEEWFKTHPTDHFADYSPDSLKKIFRKFNAELIFQKPMSYHPARDKGWRGSFPFRYF
                     YRKLADLSCYGDTVQILARKKNEI"
     gene            601..>1109
                     /locus_tag="PK683_21660"
     CDS             601..>1109
                     /locus_tag="PK683_21660"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH11119.1"
                     /translation="MKFDELSLHDALKQALRENNFTDMTPIQEQAIPAGLSGKDITGL
                     AQTGTGKTISFLVPVIDRLLNEKLEGPAALILAPTRELVLQICEEAKKLLKKTDKKVA
                     TIIGGTGYKEQEKEISEKYDIVVATPGRLIDHIRSGGLKLGNIRFFILDEADRMFDMG
                     FIKDVRYVM"
BASE COUNT          357 a          223 c          248 g          281 t
ORIGIN      
        1 tggcgagaga acgtcttgca gtactcgaaa aaaaaactcg gctctcagga ctctcactgc
       61 tcgaaatagg ctctgcagca ggcttctttc tgtcagaagc caaaaaaaaa gatttaatcg
      121 caaaagggct tgaaatctcg aatacagaag cagagtatgc aaaaagaatg gggctggatg
      181 tcgactgtgt tccatttgag gatttcaata cagaaaaaaa atttgatatc atctgtgcat
      241 ttttcgttct ggagcatttt ccggatcagg aagcctcatg gagaaaaatt ttttcattac
      301 tgcaacccgg cgggttccta tttttggctc tgccatcctt gaatggtcca acttttcgaa
      361 ctgattcaga agaatggttc aaaacccatc cgacagatca ttttgcagat tattctccgg
      421 attcactgaa aaagatcttt cggaaattca atgctgaact tatttttcaa aaaccgatgt
      481 cctatcaccc tgccagagac aagggctgga gaggcagttt cccgttcaga tacttttata
      541 gaaaattagc agatttgagc tgttacggag acacagttca gattctggca aggaaaaaaa
      601 atgaaatttg atgagctctc attacatgat gctctgaaac aggctctcag agaaaacaat
      661 tttacagata tgacaccaat ccaggagcag gcaatccctg ccgggctgtc cggcaaggac
      721 attacgggcc ttgcccagac aggcacagga aaaacgattt cctttttagt tccagtaatc
      781 gacagacttt tgaatgaaaa actggaaggt cctgcagcac tcatcctggc tcctaccaga
      841 gaactggtcc ttcagatctg cgaagaagcc aaaaaacttc tgaaaaaaac agataaaaaa
      901 gttgcaacaa tcataggcgg gacagggtac aaggaacagg aaaaggagat atcagaaaaa
      961 tacgatatcg tagtggccac gccgggaaga ctgatcgatc atatacgttc aggcggactt
     1021 aaactgggaa acatccgttt ttttatcctg gatgaggcag acagaatgtt tgatatgggt
     1081 tttataaagg atgttcggta tgtaatgag
//
LOCUS       DAOQGJ010001803         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_112764_length_1109_cov_1.843454, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001803 DAOQGJ010000000
VERSION     DAOQGJ010001803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112764_length_1109_cov_1.843454"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..972)
                     /locus_tag="PK683_21665"
     CDS             complement(154..972)
                     /locus_tag="PK683_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11120.1"
                     /translation="MQEEHISKKTETQRLHVLLGGADGLDFLERLSPHELFALRNKFS
                     AAMQNEHAATWEKLAGVAKFMPNFLNARVSQDILGPSITASLTYFIPVSEAISISSHF
                     NVSFMCDVIEHLNPAKLEPLIRDFPVDRIKKIIHEFEKRKGWYTMGNFVDYMPAEKVI
                     MISKEINSDETLIRTGSFSNEKERLIPVIRAFSDDRIRSLLQTGSSLGLCDDVLAIIK
                     YIPFEELKKYIGILVGIGGKVFQDYLGAAKKMGGEELDLVKKVTVDLGLTLDWN"
BASE COUNT          332 a          225 c          202 g          350 t
ORIGIN      
        1 tattaaaaaa tggaaataca taaaagaaac atctattgca ataataaaaa atgaattaat
       61 taatgcagag catgatttga aatgtaatta actttaatat aaaatcatca tccatgatta
      121 ataatgataa tatcattaag atgcgcatat ttttcagttc cagtcgagag tcagtccaag
      181 atccactgtg acttttttta ccaggtcgag ttcttccccg cccatttttt ttgcggcacc
      241 gagatagtcc tgaaatactt ttccgccgat tccgacaagt atgccgatat attttttcag
      301 ttcttcaaat gggatatact ttatgatggc cagcacatcg tcgcagagtc cgagactgct
      361 ccctgtctga agcagacttc tgattctgtc atctgaaaat gccctgatta ctggaatcag
      421 tctttccttt tcattggaaa aacttccggt cctgatcagt gtttcatcag aattgatttc
      481 cttggaaatc ataatgactt tttcagcagg catatagtcc acaaaattgc ccattgtata
      541 ccagcctttt cttttctcaa actcatgaat gatttttttg atcctgtcta cgggaaaatc
      601 tcggatcagg ggctccagtt ttgcaggatt cagatgctca atgacatcgc acataaaaga
      661 tacattgaag tgagaagaga tgctgattgc ctcgctgaca gggataaaat aagtcaggct
      721 ggcagtgatg ctgggtccta aaatatcctg agaaactctt gcattgagga aattcggcat
      781 aaatttggca actccggcaa gtttctccca ggtcgcagcg tgttcattct gcattgcagc
      841 cgaaaattta tttctcaaag caaagagttc gtgcggagag agcctttcaa gaaaatcaag
      901 tccgtctgca cctcccaaaa gcacatggag cctttgagtt tccgtctttt tgctgatatg
      961 ttcttcctgc atgtttcccc tctttccttt cttagcggac aggtttcaac ggaatagaaa
     1021 acattttttt ttacaagaaa aattctttaa tccgaaaaag attcacaact tgtttgcaaa
     1081 gaaatacaga taataaaatc tgtatattg
//
LOCUS       DAOQGJ010001804         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_112943_length_1108_cov_1.991453, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001804 DAOQGJ010000000
VERSION     DAOQGJ010001804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112943_length_1108_cov_1.991453"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PK683_21670"
     CDS             <1..669
                     /locus_tag="PK683_21670"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNH11121.1"
                     /translation="EGTYTRAGEISGAEYFVMIEKDSAMRLVHVQSSKVLGAWKKEDS
                     SALEDLIGRLETEKTLKDLFHLKGAGTKDFLIDITNASAAKENGGAVFGEQVKIELMV
                     KSKKHDKAYLTMLYYGQDGTVLQIFPNKHDRDNRVDTNKEISIPGEKKSYSIVASPPA
                     GTDTILLIASDSPVGLQADSASFAGPYMVQKKGLKGTKGLEVQLNKNVKYGISRILFD
                     IREK"
     gene            671..>1108
                     /locus_tag="PK683_21675"
     CDS             671..>1108
                     /locus_tag="PK683_21675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNH11122.1"
                     /translation="MKYIIMILCFGISSVSAIEPVKFKDALWNQVESVSKTDYKSYVE
                     KAGGNAGDHKGWKKTVDEVFRKLSENSGNPAFHLEYAILKEKTFNAAAFPGGQFVIHS
                     GLLDALDRMVQERKGLSESSPDFAKYRAHYLAPVIAHELGHFYN"
BASE COUNT          316 a          220 c          302 g          270 t
ORIGIN      
        1 gaagggacat atacacgcgc aggggagatt tcaggcgcag aatattttgt aatgatagaa
       61 aaggattctg cgatgcgtct tgtccatgtg cagtcttcaa aggtcctcgg tgcctggaaa
      121 aaggaagaca gttctgctct ggaggatctg atcggcaggc tcgagacgga gaagacgctc
      181 aaggatcttt ttcatctgaa aggtgcggga actaaggatt tcctgatcga cattacgaat
      241 gccagtgcgg caaaggagaa cggaggggca gtcttcggag agcaggtgaa gatagagctg
      301 atggtcaagt ccaagaaaca cgacaaggcc tatctgacaa tgctgtatta cggtcaggac
      361 ggaactgttc tgcagatatt tccgaacaaa catgacaggg ataatcgcgt cgataccaac
      421 aaggagattt ccattccggg ggaaaaaaaa tcttacagca tcgtggcttc tcctcctgca
      481 gggacggata cgattcttct gattgcatct gattccccgg tcggtcttca ggcggacagt
      541 gcctcatttg caggtcctta tatggttcag aaaaagggac tgaagggaac caaggggctg
      601 gaagttcagc tgaacaaaaa cgtaaagtac ggaatcagcc gtattctgtt tgacatcagg
      661 gagaagtagt atgaaatata ttataatgat tttatgtttt ggcatcagct ctgtttccgc
      721 aatagagccg gtcaagttca aggatgcact ctggaatcag gtcgagtctg taagcaagac
      781 tgattataag tcttacgtgg aaaaggccgg cggaaatgcc ggcgatcaca agggctggaa
      841 aaagacagtg gatgaggttt ttcggaaact ctctgaaaat tcgggaaacc ctgcctttca
      901 tctggaatat gccatactga aggagaaaac cttcaatgca gcggcttttc cgggaggaca
      961 gtttgtcatt cattcaggtc ttctcgatgc actggacaga atggtgcagg aaaggaaagg
     1021 tctcagtgaa agttcgcctg attttgcaaa atacagggca cattatcttg ctccggtcat
     1081 tgcccacgag ttgggtcatt tttacaat
//
LOCUS       DAOQGJ010001805         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_113380_length_1105_cov_4.097143, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001805 DAOQGJ010000000
VERSION     DAOQGJ010001805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113380_length_1105_cov_4.097143"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PK683_21680"
     CDS             complement(<1..400)
                     /locus_tag="PK683_21680"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HNH11123.1"
                     /translation="MNKTGSEPKDIPTVRFSKSAEPELEFEVLKLSELIRRVKNTEII
                     RRPHRLDFYNVLHITSGKGVHSIDFQKFRYNKGDTLLIGKGQVHSFLEPENAEGILTV
                     FTEKFLYKNIVKSDYLSIYSLGLYKNAAPVI"
     gene            complement(459..983)
                     /locus_tag="PK683_21685"
     CDS             complement(459..983)
                     /locus_tag="PK683_21685"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNH11124.1"
                     /translation="MIQKLIIFFTVSAFSFLPLFSQSRDSGKADIRFSIDTRFGAEKG
                     TFKKTSLKITDKKKGIGTVEIDIASIDTGNGKRDDHLRNEDFFDAPKYPKAIMEILSV
                     EINGTALKGKGKLTIKGITKDIEFAAVLAKNGETETYTGSLKLNRQEYGITYSSMMAG
                     IKDIVTVDFTVTIP"
BASE COUNT          309 a          206 c          201 g          389 t
ORIGIN      
        1 gaatgacagg agcagcattt ttatataatc ctaaactgta tattgacaga tagtcagatt
       61 ttacaatgtt tttataaagg aacttttcag tgaatacagt cagtatgcct tctgcatttt
      121 caggttcaag aaaagaatga acctgacctt tgccgatgag cagagtgtct cctttattgt
      181 aacggaattt ctgaaagtct atggaatgaa ctccttttcc tgatgtaata tggagtacat
      241 tgtagaaatc cagcctgtga ggtctccgta tgatttcggt attcttcaca cgccggataa
      301 gttctgaaag cttcagcact tcgaattcca gttcaggttc tgcggatttg gaaaacctta
      361 cagtaggtat gtctttcggt tctgaacctg ttttattcat tttattttcc atacagagct
      421 gagatcaaag caattcttta ctccgggaaa tacagatttt atggaattgt tacagtgaaa
      481 tctacagtca caatatcttt gattcctgcc atcatactgg aataagtgat tccgtattcc
      541 tgtctgttca gcttcagtga acctgtatac gtttctgtct ctccgttctt tgccagaaca
      601 gctgcaaact caatgtcttt agtaatcccc ttgatagtaa gttttccctt tcctttcaat
      661 gctgttccat tgatctcgac agaaaggatt tccattattg ctttgggata ttttggagca
      721 tcaaaaaaat cctcatttct gagatgatcg tccctttttc cgttgcctgt atcaatggat
      781 gctatgtcaa tttcaacagt cccgattcct ttctttttat ctgtgatctt gagtgaggtc
      841 tttttgaaag ttcccttttc tgcaccgaat cttgtatcaa ttgaaaatcg aatgtctgct
      901 tttcctgaat ccctggactg agaaaaaaga ggcagaaaag aaaaggctga aacagtaaag
      961 aatattatta atttttgaat catttagttc tccttgctgc cattgtctga aatcgaattc
     1021 ctgtgaaaag aattaatgaa ggcaaaattc tcaattagaa attgactgaa atattcatta
     1081 ttttattttt gagcggaaga cattg
//
LOCUS       DAOQGJ010001806         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_113442_length_1105_cov_2.512381, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001806 DAOQGJ010000000
VERSION     DAOQGJ010001806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113442_length_1105_cov_2.512381"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PK683_21690"
     CDS             complement(<1..371)
                     /locus_tag="PK683_21690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH11125.1"
                     /translation="MKLPGQKKENRKLVYCIIEDDNSFRTHLSELLKKREDTESILEY
                     QKAEDLLSLPELPRFDLLITDYRLPGMDGISLLGSPGIRQLSVPKMILSGFNAEEKTF
                     EALKYGATGYIFKEEIDQIDSV"
     gene            703..1104
                     /locus_tag="PK683_21695"
     CDS             703..1104
                     /locus_tag="PK683_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11126.1"
                     /translation="MKKIFLLLLICLSAASADNSKTAVVRFVNKKVQVQREGVPVILK
                     MGDKISFNEKVSIEENGKLEIEYEGRTFVTKKGGTVSEIISGSLSDGGFKESSVTSTV
                     GVRALDGKRKSNKKESEEGGVLDRTVSVFSE"
BASE COUNT          331 a          213 c          252 g          309 t
ORIGIN      
        1 acagaatcga tctggtcaat ttcttccttg aatatgtagc cggtcgctcc atacttcagt
       61 gcttcaaatg ttttttcttc agcattgaac ccggaaagaa tcattttagg gacagacagc
      121 tgtctgattc cggggctgcc taaaagactg atcccgtcca ttccgggaag cctatagtct
      181 gtaatgagaa gatcgaagcg aggaagctcg ggaagactca gaagatcttc cgctttctgg
      241 tattccagaa tgctttccgt gtcctctctt tttttcaaaa gctcggacag atgagtcctg
      301 aacgaattgt catcctctat aatgcagtaa accagttttc tgttttcttt cttctgtccc
      361 ggtaatttca ttgatgtaag ctgcagacct gtctgtacag tctgtgattt cctttgtagg
      421 ctgtgcagaa aaaaagacaa tcaaaaaaaa attaattacg gaatatctgt aatatttgcc
      481 aattgatcaa tcatcggctt tccttttacc gaagataagg ctgatatatc atatattcca
      541 atactgtcct gaaaaagata actgctcaat tttagaaaca gcatccttgc attgagcaca
      601 ataagctgtt cagatgaact ccgatatcag gcaaaaaaat tacttgacct gtctgtattc
      661 tgcgccctag acttgcagat attccgattc gggaggattg aaatgaagaa aatatttctg
      721 ctgctgctca tatgcctgtc tgccgcttct gcagataatt caaagacggc cgttgtccgg
      781 tttgtcaata agaaagtgca ggtgcagagg gagggtgttc cagtcatttt gaaaatggga
      841 gataagatct ctttcaatga aaaagtgagc atagaggaaa atggaaaact ggaaattgaa
      901 tatgaaggaa gaacatttgt cacaaagaaa ggcggaactg taagtgagat tataagcgga
      961 agcctttccg acggaggatt caaggagagt tcagtcactt ccactgtagg ggtgcgggca
     1021 ttggacggaa agaggaaatc caataagaaa gaatccgaag aaggcggggt gctcgacagg
     1081 acagtatcgg ttttctcgga ataac
//
LOCUS       DAOQGJ010001807         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_113587_length_1104_cov_3.174452, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001807 DAOQGJ010000000
VERSION     DAOQGJ010001807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113587_length_1104_cov_3.174452"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1104
                     /locus_tag="PK683_21700"
     CDS             <1..>1104
                     /locus_tag="PK683_21700"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNH11127.1"
                     /translation="HADGEIKGIAFEGEILESYLKKSEDFLERTEPEMEIYRTKIEEC
                     SGKSICDELYVTKINSSEYFTPAEKALAEKTGLHYSESKAAFLLFMKTVSGSELLISM
                     LPLQEKIINSAVKEREFVFFINKKGYVFLNLNTPYAKEGSKISIHPEDLKNIGSELQS
                     GSFNMKSADSSTLNVFQKLSFLNVVMVYGNENKAAYSDIDRIRSFAGRILLFMLPVIL
                     SLAYFYSRWSKSRFNMLRRTVQDIKESKFETRIELEKTRLQDEVIDLSAAVNEMAEKL
                     NSDHKKTMEKLLLMNEKLQETNSFLAVSENAANQASRVKSLFLANMSHEIRTPMNGIM
                     GMCQLLLETKLEEEQKEYLEIIYSSSENLLALI"
BASE COUNT          387 a          215 c          223 g          279 t
ORIGIN      
        1 atcacgccga cggcgagatc aaaggcattg cctttgaagg cgaaattctt gaatcctatc
       61 tgaaaaaatc agaagatttt ctggaaagaa cggaacctga aatggaaatt tacagaacca
      121 agattgaaga gtgttcagga aaatcaatat gtgacgaact ctatgtcaca aaaataaatt
      181 ccagtgaata ttttactccc gcagaaaaag cgctcgcaga aaagacaggc ctgcattatt
      241 ccgaatcaaa agcagcattc ctgctcttca tgaaaacggt ttccggttct gaacttctga
      301 tatcaatgct tcctcttcag gaaaaaataa ttaattcagc agtaaaggaa agagaatttg
      361 tattttttat aaataaaaag ggatacgttt tcctgaatct gaacacaccg tatgcaaagg
      421 aaggctcgaa gatcagcatt catccggaag acctgaagaa cataggctca gaacttcaga
      481 gcggcagttt caacatgaaa tcagccgact cctccacact gaatgtattt cagaagcttt
      541 catttctgaa tgtagtcatg gtttacggga atgaaaacaa ggcggcgtac agtgatatag
      601 acagaatccg aagttttgca ggcagaatac ttctgtttat gcttcctgta attctttcac
      661 tcgcttattt ttattccaga tggagcaagt ccagattcaa tatgcttcgc aggactgtac
      721 aagatattaa ggaatccaaa ttcgaaacca ggattgaact tgaaaaaaca aggcttcagg
      781 atgaggtcat tgatctttcg gcggcagtca atgaaatggc tgaaaagctg aacagtgatc
      841 acaagaaaac aatggaaaaa ctcctcctga tgaatgaaaa acttcaggaa acaaattcat
      901 tccttgcagt ttcagaaaat gcggcaaacc aggccagcag agtaaaatca ctttttcttg
      961 caaatatgag tcatgagatc cgaacaccga tgaacggaat tatgggaatg tgccagctcc
     1021 tgctggaaac aaaacttgag gaagaacaga aggaatatct ggaaatcatt tactcaagtt
     1081 ccgaaaatct tcttgcactc atca
//
LOCUS       DAOQGJ010001808         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_113725_length_1104_cov_1.630124, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001808 DAOQGJ010000000
VERSION     DAOQGJ010001808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113725_length_1104_cov_1.630124"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PK683_21705"
     CDS             complement(<1..615)
                     /locus_tag="PK683_21705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11128.1"
                     /translation="MDKSYFAVGLNDSIEGGRVAGQISLSTETVTFSYAGGSVRLPAK
                     GIHIEQGGSGGSLIYFSHESEKDWIIYTSDKSVLRDRAISGAPALGELKTQRIRRDMK
                     IAAAVFLLALSVTGIIYLFKFYKETFTFFLASQIPVKWEKSLGQNVYKSLTAGKKMYD
                     SEETLKLLEPITKPLTEAAKHSGYEFKFHIMEDSRVNAFAIPGGN"
     gene            complement(730..>1104)
                     /locus_tag="PK683_21710"
     CDS             complement(730..>1104)
                     /locus_tag="PK683_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11129.1"
                     /translation="QKAGFPRFKGKYRYSSFTYPGTGWELMGDKLKLSKIGTMRVMFT
                     RPIEGTIKTVQIKREVDFFYVIFTCEIKNNQQVNQDKLLKIKAGNCSALKTSMHIENF
                     ILFLANGIFQPKNGIHQPFFSV"
BASE COUNT          293 a          237 c          207 g          367 t
ORIGIN      
        1 atttcctccc ggaatggcaa atgcatttac acgcgagtct tccatgatat ggaatttgaa
       61 ttcgtatccg gaatgttttg cagcctcagt gagaggtttt gtaatcggtt ccagaagttt
      121 cagtgtttct tccgaatcat acattttctt tcctgcagtg agggacttgt atacattctg
      181 tcctagagat ttttcccatt ttaccgggat ctgggatgcc aggaaaaaag tgaatgtttc
      241 cttgtagaat ttaaaaagat agataatccc tgtcactgat aaagccagaa gaaatactgc
      301 agcagcaatt ttcatatccc ttctgattct ctgcgtcttc agttccccca gtgcaggagc
      361 tccggaaatg gctctgtccc gaagaacaga tttgtctgaa gtgtatataa tccagtcttt
      421 ttcggattca tgtgagaaat agatcaggct tcccccgctc cctccctgct ctatatgaat
      481 tccctttgca ggaagcctca cgcttccgcc tgcataggaa aatgtgactg tttctgtgct
      541 caaggagatt tgaccggcaa ccctcccgcc ttctatggaa tcattcagac cgactgcaaa
      601 ataactcttg tccattcttt ctcctgtttt ggtttcccct gccaagactc tgattctcgg
      661 gaagtttcat caatctttat tttagaggat tttttttgca gaaccggaaa aaactttgga
      721 atgacagatc taaaccgaaa agaaaggctg gtggattcca ttttttggct gaaatattcc
      781 atttgccaaa aataatataa aattctcaat gtgcattgag gttttcaatg cagagcaatt
      841 accagccttt attttcagaa gcttatcttg gtttacttgt tgattatttt ttatttcaca
      901 agtaaagatc acatagaaaa agtcaacttc tctctttatt tgtacagttt taatagtacc
      961 ttctattgga cgagtaaaca ttactctcat agtacctatt ttagagagtt taagcttatc
     1021 tcccattaat tcccaaccag ttccagggta ggtgaagcta ctgtagcgat atttgccctt
     1081 gaacctggga aaacctgctt tctg
//
LOCUS       DAOQGJ010001809         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_113820_length_1103_cov_2.811069, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001809 DAOQGJ010000000
VERSION     DAOQGJ010001809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113820_length_1103_cov_2.811069"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PK683_21715"
     CDS             complement(<1..781)
                     /locus_tag="PK683_21715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH11130.1"
                     /translation="MKDLKMAKIIVIDDSESYLEKVKDILELENEYEVFTASSAGAGL
                     EIIRKEKPDLIVSDIDMPEMNGFELFNELKLDPETELCPVIFLTAMNSMDDLRYGMSL
                     GVDDYIVKPIKAEDFLSSVKVRLSRSRRLKSRLNRSDAESREYKDLKNDLMIAKQTQE
                     GFLPSRDRQFSGIDFEYAYLPMIDVGGDFFSITELNDGRIRIFFSDALGHGISASIFN
                     MAIKVEYDSLKNSPDSPSVILSELNKRFCDKKTITDKNIFFI"
BASE COUNT          305 a          245 c          186 g          367 t
ORIGIN      
        1 ttatgaaaaa aatattttta tctgtgattg tttttttatc acagaatctt ttattcagtt
       61 cagacagtat gacagacgga gagtctggac tgttttttaa gctgtcgtat tccaccttga
      121 ttgccatatt gaatatagaa gcactgattc cgtgcccaag cgcatctgaa aagaaaattc
      181 gtattcttcc gtcattcaat tctgtaatgc tgaaaaaatc gccgcctaca tcgatcattg
      241 gaaggtaagc atactcaaag tcaatccctg agaactgtct gtccctggaa ggaagaaaac
      301 cttcctgtgt ctgttttgca atcatcagat catttttcag atccttatat tcccggcttt
      361 ccgcgtctga tctgttcagt cttgacttca gtcttctgct tctgctgagt cttactttca
      421 cagaagaaag gaagtcttct gccttgatcg gttttacgat atagtcatcg acacccagac
      481 tcattccata tctcagatca tccattgaat tcattgctgt taaaaatatg acaggacaga
      541 gttctgtttc cggatccagt ttcagctcat tgaaaagctc aaatccgttc atctccggca
      601 tatcaatatc tgaaacaatc agatccggtt tttcttttcg aattatctcc agacctgctc
      661 ctgcacttga ggcagtaaac acttcatatt cattctccag ttcgagaatg tccttcactt
      721 tttccagata actttctgaa tcatcaatta caataatctt agccattttc aaatccttca
      781 tcagtaaatc ctgaacctgc agaaaaattc attctatgtg caccccggca gggagtcata
      841 ttctgcaatt gtacaaattc ccgaaacgga attgatatgg ttcttatgat tcttgatcca
      901 gaacaaccta tattgtctct atatatgata cagttgaaga cattgaatcc gagtccagtg
      961 aaatattttt atattccttt gcagatatgt ctgaaagcgc agtcgaaaga ctcttaaaga
     1021 gccgtatatt gccgcttttt tcagaccttc ggactgagtg tcagttctta aacagtcccg
     1081 ttcctcaggt ttgagaattc cag
//
LOCUS       DAOQGJ010001810         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_113847_length_1103_cov_2.437977, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001810 DAOQGJ010000000
VERSION     DAOQGJ010001810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113847_length_1103_cov_2.437977"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PK683_21720"
     CDS             complement(<1..1071)
                     /locus_tag="PK683_21720"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNH11131.1"
                     /translation="MLFPEPGDQGRMNSCTAFATAYHLKSYMEAVDQGWEPRTQNKIF
                     SPAFVYNQINGGQDYGSSIYNALELLRTKGCSTLQSTPYKAGDYRSQPSQNALKEAAQ
                     YKIKGFQAVKNLAGIKAALQEGLPIVAGITTDPVFMYGKFDVYGKGKFKRQELGHCSY
                     EAHGRHAVIIAGYDDTKQALLLLNSWGKGWGNAGYGWISYDLINRIAYHDECKNFMET
                     AFVAFDEKNKVNAAKYDSEIYGSILYLGLSKKNQPVWSWKLGLKGALDNISSIKWDID
                     PSLQISESEEEKKDDFRTAGTASSPGKKRARAEIVFKDGKKEVKEYFLNFQLSNRRTL
                     EIHLTDEYIGLYKGKRNYRWNLS"
BASE COUNT          260 a          266 c          218 g          359 t
ORIGIN      
        1 cgaaagattc catctgtagt ttcgttttcc tttataaaga ccgatatatt catcagtcag
       61 atgaatttcc agagttcttc tgttcgagag ctggaaattc agaaaatatt ccttgacttc
      121 cttctttcca tccttgaaga caatttcggc cctggctctc ttttttccgg gagaagatgc
      181 agtaccggct gttctgaaat catccttttt ttcttcctct gattcagaaa tctgcaggga
      241 aggatctata tcccatttta tggatgaaat attgtctaag gctcctttca gacctaattt
      301 ccagctccag acgggctgat tttttttgga aagtccaagg tacagaatgc ttccatagat
      361 ttctgaatca tattttgcag cattcacttt gttcttttca tcaaaggcaa cgaatgccgt
      421 ttccatgaaa tttttacatt catcatgata agctatcctg tttatcagat cataggaaat
      481 ccatccgtac cctgcatttc cccagccctt tccccaggag ttcagaagaa gaagcgcctg
      541 ttttgtatcg tcgtatcccg caatgatgac tgcatgtctt ccgtgagcct cgtaagagca
      601 gtgacccaat tcctgtcttt tgaattttcc ttttccatac acgtcaaatt tcccgtacat
      661 gaacacaggg tctgtcgtaa ttcctgcaac aatcggaaga ccttcctgaa gagctgcctt
      721 aattccagca agatttttga ctgcctgaaa tcctttgatt ttgtactgtg ccgcttcttt
      781 cagtgcgttc tgggacggct gagaacggta gtctcctgcc ttgtaaggag tgctctgaag
      841 ggtagaacat cccttggtcc ggagcagttc aagtgcattg tagatgctgc ttccgtaatc
      901 ctgccctccg ttgatctgat tgtatacgaa tgcaggagag aatattttat tctgtgtcct
      961 gggttcccag ccctggtcta ctgcttccat ataacttttc aaatggtacg cagttgcaaa
     1021 agcagtgcag gaattcattc ttccctgatc gcccggttca ggaaaaagca tccttccctc
     1081 ggaatccctt ctcaggtctg cag
//
LOCUS       DAOQGJ010001811         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_113852_length_1103_cov_2.369275, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001811 DAOQGJ010000000
VERSION     DAOQGJ010001811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113852_length_1103_cov_2.369275"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1103
                     /gene="asnS"
                     /locus_tag="PK683_21725"
     CDS             <1..>1103
                     /gene="asnS"
                     /locus_tag="PK683_21725"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNH11132.1"
                     /translation="IQFLQVRNSGYIVQAVCEKEKFSEDEFQKIKSLAQESSIEITGK
                     VVRSEKSDSGYEIQVSSFSLINDSKDFPITPKEHGVDFLHNNRHLWLRSRKQLAILRI
                     RNEISFAIRKYFYERDYVLLDSPILTGSVGESAGTLFELEYFDEGKAFLAQTGQLYLE
                     TGIFAHDKVYCFGPTFRAEKSKTRRHLTEFWMLEAETAHFDNDANIDLQEDFVKYIIS
                     HVLKRCVQDMKILERNPDKLERQMSKPFPRITYTEAIEILKSKGQTISWGEDINAERE
                     LILTEHFSMPVFIMNYPRAIKAFYMKQNPKDPRTVLCADLIAPEGAGEIIGGSEREEN
                     LNKILDLIREEKLPEESYSWYLDLRKYGSVPHSG"
BASE COUNT          358 a          232 c          245 g          268 t
ORIGIN      
        1 atccagtttc tgcaggtcag aaattcagga tacattgtgc aggctgtctg cgaaaaggaa
       61 aaattttccg aagatgaatt tcaaaaaatc aagtcccttg ctcaggaaag ttcaattgag
      121 atcacaggaa aagtagtccg ttcagaaaaa tctgactcag gatatgaaat tcaggtaagc
      181 tccttcagtc ttataaatga ttccaaagat ttcccgatca ctccaaaaga acacggtgtg
      241 gactttcttc acaacaacag acatctctgg ctccgctcca gaaagcagct ggcaatttta
      301 agaatacgga atgaaatcag ctttgcaatc cgtaaatact tttatgaaag agattatgtt
      361 ctgctggatt caccgattct tacaggttcc gtaggcgaaa gcgcgggaac ccttttcgaa
      421 ctggaatact ttgatgaggg gaaggcattt ctcgcccaga caggccagct ttatctggaa
      481 accggcatat ttgctcatga taaggtatac tgcttcggtc ctacattcag ggccgaaaaa
      541 agcaagacca gaagacatct gacggaattc tggatgcttg aagcagagac agcacatttt
      601 gacaatgacg caaacataga ccttcaggaa gactttgtaa aatatatcat atcccatgtt
      661 ctgaaaagat gcgttcagga tatgaaaata ctggagagaa atcctgataa actggaaaga
      721 cagatgtcca aaccttttcc cagaatcacc tacactgaag caatcgaaat cctgaaatcg
      781 aagggtcaga ctatttcctg gggagaggac ataaacgcag aaagggaact gattctgaca
      841 gaacattttt ctatgcctgt tttcattatg aattatccca gagccataaa agcattttat
      901 atgaaacaga accctaagga ccctagaaca gtgctctgtg cagatctgat tgcaccggag
      961 ggtgccggtg agatcatcgg gggttcagaa cgggaggaaa acctgaataa gatactggac
     1021 ctgatcaggg aagaaaaact tccggaggaa tcctattcct ggtacctgga tctcagaaag
     1081 tacggatcgg tcccccactc cgg
//
LOCUS       DAOQGJ010001812         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_113989_length_1102_cov_3.214900, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001812 DAOQGJ010000000
VERSION     DAOQGJ010001812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113989_length_1102_cov_3.214900"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1102
                     /locus_tag="PK683_21730"
     CDS             <1..>1102
                     /locus_tag="PK683_21730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH11133.1"
                     /translation="MLDAEGRIATWNRGAERIFGYSEEEIIGESITKIIPVDRIQEEK
                     FIFDETLSGRILNHYETVRLDKKGNEIYVSITASPLMDTENRIIGVSKISRNISEKVR
                     ILKELKDAKTAAEQASKVKSEFLANMSHEMRTPLNGVMGFSDLLLSTKLDSLQRYYVN
                     TILQSSEFLLGIINDILDISKIEAGKLEIYEEVCDLVEICKFAADTVRTQSESKELEL
                     SLHIDPDLTVSVFADSFRLKQILINLLGNAVKFTESGTVELDLKLLNRMDDGEISVRF
                     SIMDTGLGIDPKNQERIFEAFYQADLSSVRKFGGAGLGLTISSRILSLMDSSLKVRSE
                     LGRGSTFYFDLKMKTSPEKNQFEKSMHISPYSL"
BASE COUNT          353 a          199 c          250 g          300 t
ORIGIN      
        1 catgctggac gcggaaggcc gcatcgccac ctggaacagg ggtgcggaac ggattttcgg
       61 gtattcagaa gaggagataa tcggagaatc aataactaaa atcattccag ttgaccgaat
      121 tcaggaagag aaattcattt ttgatgaaac cctttccggc aggatactga atcattatga
      181 aacagtcaga ctcgataaaa aaggaaatga gatttatgta tccattactg catctcctct
      241 gatggatacc gaaaacagaa ttataggagt atcaaaaata tccagaaata tttctgagaa
      301 agtaagaatt ctgaaagaac tgaaggatgc aaagaccgca gcggagcagg ccagcaaagt
      361 aaaatcagag tttcttgcca atatgagcca tgaaatgaga actccgctga acggcgttat
      421 ggggttttcc gatctgcttc tttcgacaaa actggattca ctgcagagat actatgtaaa
      481 tacaatcctt cagtcttctg agtttctgct gggaatcatc aatgacattc tggatatttc
      541 aaaaattgaa gcaggcaaac tggaaattta tgaagaggtc tgtgatcttg ttgaaatctg
      601 caaatttgca gcggatacag tccggactca gtcggagtcc aaggagcttg aactttccct
      661 tcatattgat cctgacctga ctgtaagtgt ctttgcagat tctttcagac tgaaacagat
      721 actgatcaac cttctgggca atgcggtaaa attcactgaa tctggaacgg ttgagctgga
      781 tctgaagctg ttaaacagaa tggatgatgg agaaatttct gtccgttttt ccattatgga
      841 taccggactt ggaatagatc ctaaaaatca ggaaagaata tttgaggctt tttatcaggc
      901 tgatttgtct tctgtgagaa aattcggcgg ggcaggactt ggacttacca tttcgagcag
      961 gatactttct ttaatggaca gctcacttaa agtaagaagt gaattaggga gaggcagtac
     1021 tttttatttt gatctgaaga tgaaaacctc accggaaaaa aaccaatttg agaaatcaat
     1081 gcatatcagt ccttacagtc tg
//
LOCUS       DAOQGJ010001813         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114025_length_1102_cov_2.556829, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001813 DAOQGJ010000000
VERSION     DAOQGJ010001813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114025_length_1102_cov_2.556829"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..>1102
                     /locus_tag="PK683_21735"
     CDS             50..>1102
                     /locus_tag="PK683_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11134.1"
                     /translation="MSSGLRDQRSENEPQNSAKHSVLAALCADAQTQQASLRSPGEVL
                     SGLKRGISIGIGFMLTLGVSAVLAVAVTGTINTYTNGAVMDAASLNTNFASLKTAVEG
                     ITSSQWTNNGTSIYYSGGSVGIGTNSPSTSPPAGRSVPVTAAVYTDFSTDSVDLDIRG
                     QYAWNAFGVGNHGRGSTVRLYSGNTNTSYIYSNIYNGSALSLSSNFYYGIHGNTYIPN
                     PGNATSQVLIGDGDINFQTGATNTAPTTKMYVAASGNVGIGTTSPTAPLEVSGNITLS
                     NGNRQILFSDTGNYDFSIVHNGGTSLDFKSPEFSASAALSIHNSARVGIGTNSPSANL
                     HVVGTTLATAWSTSDER"
BASE COUNT          290 a          252 c          273 g          287 t
ORIGIN      
        1 cgggattatg aaaaggactt tatcattttt ttttagggcg tgggaaagga tgagttcagg
       61 gctcagggat cagcgctcgg agaatgaacc gcagaattca gcaaagcatt ccgtcttagc
      121 cgccctttgc gcagatgcgc aaacccagca ggcctccctt cggtcgccgg gtgaggttct
      181 gtccggtctg aaacgcggga tatccattgg catcggcttt atgctcacac ttggagtctc
      241 cgctgtgctc gcagtggcag tgacaggaac aatcaataca tatacaaacg gtgctgtgat
      301 ggatgcggct tccctgaata cgaattttgc ttcgttgaag actgccgtcg aaggcatcac
      361 tagttctcag tggacgaaca acggaacgag tatttattac agcgggggga gtgtaggaat
      421 tgggaccaac agtccgagta caagtccgcc tgccggaaga tcagttcctg tcacagctgc
      481 tgtatacacc gatttttcga cagattcagt tgatttagac atacggggac agtatgcctg
      541 gaatgcattc ggagtgggta atcacggcag aggttctact gtgcgcttat attcgggaaa
      601 cactaataca tcttatatat acagcaatat ttataatgga agtgctcttt ccctttcatc
      661 aaatttttac tacggaatac acgggaatac atatatcccg aatcctggaa atgcaacttc
      721 tcaagttttg ataggagacg gcgatatcaa ctttcaaaca ggtgccacaa atacggctcc
      781 tacaacaaaa atgtacgttg ccgcctcggg taacgtcgga attggcacga cgagtccgac
      841 agcaccttta gaggtatcag gaaatattac gttatctaac ggaaacaggc agattttatt
      901 cagtgatacg gggaattatg atttcagtat tgttcacaac ggtggtactt ctttggattt
      961 taagtcaccg gaattcagtg cttctgctgc attgtctatt cacaatagcg ctcgtgtcgg
     1021 catcggcaca aacagcccat ccgcgaacct tcacgttgtc ggcactacac tggccacagc
     1081 ctggagcact tcggatgaac gt
//
LOCUS       DAOQGJ010001814         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114292_length_1101_cov_1.970363, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001814 DAOQGJ010000000
VERSION     DAOQGJ010001814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114292_length_1101_cov_1.970363"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /gene="ispG"
                     /locus_tag="PK683_21740"
     CDS             complement(<1..790)
                     /gene="ispG"
                     /locus_tag="PK683_21740"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020783453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNH11135.1"
                     /translation="MNPYTFSPFAYRRFETREVKVGDLGIGGKNPIRVQSMTTSDTRD
                     TESTVRQILDLERAGCELVRLTVPSMADAENLPNIRRRLKEENSKVPLAADIHFTPSA
                     AMLAAEYMEKVRINPGNFADRKKFAVREYTDSEYNEELERISEVFLPFVKKCKELGRA
                     VRIGTNHGSLSDRIMNRFGDTPLGMVESALEFIRIAETVNFHDIIVSMKASNPQVMVH
                     AYRLLVSRFIELGMKYPVHLGVTEAGDGKDGRIKSAMGIGTLLED"
     gene            complement(787..>1101)
                     /locus_tag="PK683_21745"
     CDS             complement(787..>1101)
                     /locus_tag="PK683_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNH11136.1"
                     /translation="ILSNVFMTFAWYGHLKYTDFPLWKVILISWGIAFFEYILQVPAN
                     RIGHETFTAGQLKVMQEVISLSVFGIFSLTYLHEKLRWNDIVGFLLILLAVFVVFKKW
                     WI"
BASE COUNT          276 a          262 c          248 g          315 t
ORIGIN      
        1 cgtcttccag aagagttcct atccccattg cagatttgat tcttccgtct ttgccgtctc
       61 cggcctctgt gactcccaga tggaccggat atttcattcc cagttcaatg aacctcgaca
      121 caaggagtct ataggcgtgc accatcacct gaggattgga tgccttcatg gaaactataa
      181 tatcatgaaa attgacagtt tctgcaatac ggatgaattc cagtgccgat tcgaccattc
      241 cgaggggagt gtctccgaaa cggttcatga tcctgtcgga aagagaaccg tggtttgttc
      301 cgattctcac tgcgcgtccg agctccttgc atttttttac gaacggaaga aatacttcag
      361 aaatcctttc gagctcctca ttgtattcag agtcagtgta ttcacggact gcaaactttt
      421 ttctgtcggc gaagtttcca ggattgattc tcactttttc catgtactct gctgcaagca
      481 ttgctgcgga aggagtgaag tggatgtctg ctgcaagagg cactttcgaa ttttcctcct
      541 tcagtcttct gcggatgttc ggaagatttt ccgcatctgc catggaagga acagtgagcc
      601 ggacgagttc acacccggct ctttccagat caaggatctg ccgtaccgtg ctttccgtgt
      661 ctcttgtgtc tgaagtggtc atagactgaa cccggatcgg attttttcct ccgattccga
      721 gatcgcctac tttgacttct ctggtctcaa aacgtctgta tgcaaaagga ctgaatgtat
      781 acggattcat atccaccatt ttttaaagac tacaaaaacg gcaagcagta tgagaaggaa
      841 tccgacgatg tcattccatc tgagtttttc gtgcagatag gtgagagaaa aaattccgaa
      901 gaccgaaagg ctgatcactt cctgcatcac tttcagctgt cctgcagtga atgtctcgtg
      961 tccgattctg tttgccggaa cctgcagaat atattcaaag aaggcgattc cccagctgat
     1021 gagaatgacc ttccagagag gaaaatcagt atatttcaga tgaccgtacc aggcgaatgt
     1081 cataaacaca ttggataaga t
//
LOCUS       DAOQGJ010001815         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114382_length_1100_cov_3.288038, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001815 DAOQGJ010000000
VERSION     DAOQGJ010001815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114382_length_1100_cov_3.288038"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PK683_21750"
     CDS             complement(<1..835)
                     /locus_tag="PK683_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11137.1"
                     /translation="MGMEDREGARNRERDLVLSPNEYAFISDQTKGNINVYVGPYKTS
                     LANTDLPVFFDEKTKRFQSSILEKSIQTFSIAPENWYITLKNPAKDNSHPKNGTVNNL
                     TELNTGHKINIPGPVSFALFPGQMAKVIKGHHLRSNQYLVVRVYDENSAKENWSKGSV
                     VKTNEDSGNAGFNVDQLTLGKLFIIKGTEVSFYIPPTGIEVVKDSRGEYIRNAVTLER
                     LEYCILLDESGNKRYVRGPDVVFPEPTENFLEKNGNRKFKAFELSEIKGLYIKVIDSY
                     TD"
BASE COUNT          279 a          259 c          201 g          361 t
ORIGIN      
        1 tgtccgtata agagtcgatg accttgatgt aaagcccttt gatttcagaa agctcgaaag
       61 ccttgaactt cctgttgccg tttttctcga ggaagttttc agtcggttca ggaaaaacta
      121 catcaggtcc gcgcacatat cttttgtttc cgctctcatc cagaagaatg cagtattcca
      181 gtctctcaag agtcactgca tttctgatat attctcctct tgagtccttg acgacttcaa
      241 tccctgtagg cgggatataa aaagatactt ctgttccctt tataatgaaa agctttccga
      301 gtgtaagctg atccacattg aatcccgcat tccctgaatc ttcgttggtc tttaccacag
      361 aacctttcga ccagttctcc tttgcagaat tttcatcata cactcttacg accaggtact
      421 gattggatct caggtgatgg cccttgatga cctttgccat ctgacccggg aaaagagcaa
      481 aggatacagg tcccgggata ttgatcttgt gacctgtatt cagttcagtc agattgttga
      541 ctgttccgtt cttaggatga gaattatcct tcgccggatt cttcagtgta atgtaccagt
      601 tttcaggagc tatcgaaaaa gtctgaatgg acttttcaag aatgctgctt tgaaatcttt
      661 tcgtcttttc atcaaaaaaa acaggcagat ccgtgtttgc cagactcgtc ttgtaaggac
      721 ctacatacac attgatgttt ccctttgtct ggtctgaaat aaatgcatat tcattcgggc
      781 ttaaaaccaa atctctttct ctgtttcttg ccccttctct gtcttccatt cccatagtat
      841 tctcctttgt ataaatcaaa acgaattcga tactgcatcg aatttatgcc tgctttaaaa
      901 aagcaggaaa accttgttcc ctatcgggca gatttatggg tttaacaacc cctaaaaccc
      961 cgtttttttc tttcatcgaa tttatgcctg ctttcataat agctcctgcc aggcagtaag
     1021 ggcatatatc tgcaaatctg atgccttaac agcgtcttga taagaccttt atatttctcc
     1081 ataaacctga ctgcaggccg
//
LOCUS       DAOQGJ010001816         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114417_length_1100_cov_2.826794, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001816 DAOQGJ010000000
VERSION     DAOQGJ010001816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114417_length_1100_cov_2.826794"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="PK683_21755"
     CDS             <1..141
                     /locus_tag="PK683_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HNH11138.1"
                     /translation="LYEKGRLNLSRSITSRHSIEEVNDCLENLYRRTGNPIRFIIEPG
                     SA"
     gene            434..>1100
                     /locus_tag="PK683_21760"
     CDS             434..>1100
                     /locus_tag="PK683_21760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH11139.1"
                     /translation="MKEDSRLKTLRSFMEFVPDPMFLCDRTGRILLYNAKSLLFFEFE
                     KTPETDILVHSILMEEFLPEFSIFWKECFSDENEKKTEKLEMICRCRDGRILPFEIKA
                     GVSEIENEKYAAFTFRNISDRKDAEKDLLETEKKFHAIFEQAAVGVALIASETGEFIS
                     INRKYCDIVGYSIREMTGATFLQITHPDDLQRDLNNMKNLILGKIHSFSMEKRYFHKD
                     GSIV"
BASE COUNT          352 a          221 c          239 g          288 t
ORIGIN      
        1 ctttatgaga agggcagact gaatctttcc cgttcaatca cttccagaca ttcgattgaa
       61 gaagtgaatg actgcctgga aaatctttac aggagaaccg gaaaccccat aagattcatc
      121 atagagccgg ggtctgcctg acagaactat ggctgctgca gataacaata ttttttttta
      181 caatctcagg ctgcgcaaat acgatatgct gtccagatgc tgtaattttc ataaaacatc
      241 agtaaaatcc agcagtttaa cagcttgcgg tgaatcagta aaaacagcga cttccacgct
      301 tgcgggattt tcagcagttt ccaagttcgc gagggcagag ccctcagcag cctcaaggtt
      361 tgcgaaaccg ccgacaggct ctctgcagcc ggaggtgtaa ggtgagtcac aatataagac
      421 aggtatgttc cggatgaaag aagattctcg tctgaaaaca cttcgaagtt ttatggagtt
      481 tgtgcctgac cctatgtttt tatgcgaccg gacaggcaga atacttcttt ataatgcaaa
      541 atcgctcctg ttttttgaat ttgagaaaac tccagaaacg gatattctcg tccattccat
      601 acttatggaa gaatttctgc ctgaattctc aatattttgg aaagaatgct tttcggatga
      661 aaatgaaaaa aaaaccgaaa aactggaaat gatatgcaga tgcagagacg gacgcattct
      721 tccttttgaa atcaaggcgg gagtttccga gattgaaaat gaaaaatatg cagcttttac
      781 tttcagaaat atttcagaca gaaaggatgc ggaaaaagat cttctcgaaa cagaaaaaaa
      841 atttcatgcc atttttgaac aggcagcagt aggcgttgcg ctgattgctt ctgaaacagg
      901 agaattcata tccatcaaca ggaaatactg cgacatcgtg ggatacagca tcagggaaat
      961 gaccggtgcc acatttcttc agatcactca cccggacgat ctgcagaggg acctgaacaa
     1021 tatgaaaaat ctcattctag ggaaaattca ttccttcagt atggaaaaaa gatacttcca
     1081 taaggatggg agcattgtct
//
LOCUS       DAOQGJ010001817         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114603_length_1099_cov_2.952107, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001817 DAOQGJ010000000
VERSION     DAOQGJ010001817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114603_length_1099_cov_2.952107"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PK683_21765"
     CDS             complement(<1..1024)
                     /locus_tag="PK683_21765"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH11140.1"
                     /translation="MKYELNLDFIFQKKTEYISLLYTLPVFCSFVLQFSHIRRGAFEK
                     ILNGVLHIICLVYIILDDMRTLDIINRNIVHPLFAVYCIYTVRCLLQEISSKDREDIK
                     YLLYSTLFLGACTVLDILRARGLVQIRPIFDYAFFVFILGISAILVNRFVRMKNLLDE
                     INRNLETKVKERTKELEDSYLVLKELKNRQDADYFLTSLLLKPLQIQNYETDLMKVEF
                     FTIQKKIFEFRGERHEIGGDLNMADRITLEGKKYLFFCNSDAMGKSIQGAGGALVFGS
                     VLRTLIYKAHTRFYKNCSPERFLKDVFIELQRVFEGLNGVMLVSALIGVVEEASGLLY
                     YINSEHP"
BASE COUNT          333 a          231 c          215 g          320 t
ORIGIN      
        1 agggatgttc tgaattgata tagtaaagaa gtccagatgc ctcttccaca actccgatca
       61 gtgcagacac aagcatgact ccgttcagtc cctcgaatac tctctgcagt tcaatgaaca
      121 catccttcag aaaacgttca ggagaacagt ttttataaaa acgggtatga gccttgtaga
      181 ttagggttcg gagaacactc ccgaatacga gtgcgcctcc tgcgccctga attgattttc
      241 ccattgcatc tgaattgcag aagaagagat attttttccc ttccagtgtg attctgtccg
      301 ccatattcag atcgcctcct atttcatgac gttcccctct gaattcaaaa atctttttct
      361 ggatcgtaaa aaattccact ttcatcagat cagtttcata gttctgaatc tgcagaggct
      421 tgagaagaag tgaggtgaga aaatagtctg catcctgacg gtttttcagt tccttcagca
      481 caagatagga gtcttcgagt tcctttgtcc gttccttcac ttttgtctcc aggttcctgt
      541 tgatttcatc cagaagattt ttcattctga cgaaacggtt cacaaggatg gcggaaattc
      601 ccaaaatgaa aacaaagaat gcatagtcga aaataggacg gatctgcaca agtcctctgg
      661 cacgcagtat gtccagcact gtgcaggctc cgagaaacag tgttgaatag agaagatatt
      721 ttatatcctc tctgtctttg gaagagattt cctgaagaag gcatctgact gtataaatgc
      781 agtataccgc aaacagggga tgcacaatat tcctgttgat gatgtctaga gttctcatat
      841 catccagtat aatatataca agacagataa tatgcaatac tccgttcaga atcttttcaa
      901 aagctcctct tcggatatga ctgaactgaa gcacgaaact gcagaataca ggaagtgtat
      961 ataaaagtga gatatattct gtttttttct gaaatatgaa gtccagattc agttcatatt
     1021 tcatctgacc cctcaggaaa agatagcgca aataaagtca cacgatttaa tgtataaccc
     1081 ataaagtaat atatggcca
//
LOCUS       DAOQGJ010001818         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114693_length_1099_cov_2.119732, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001818 DAOQGJ010000000
VERSION     DAOQGJ010001818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114693_length_1099_cov_2.119732"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /gene="tpx"
                     /locus_tag="PK683_21770"
     CDS             complement(<1..494)
                     /gene="tpx"
                     /locus_tag="PK683_21770"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNH11141.1"
                     /translation="MAKVTLKGNEISTSGDLPKKGDSAPDFTLTKADLSDATLKSFSG
                     KKLVLNIFPSIDTAVCATSVRKFNEQASKLENTAVLCISADLPFAQSRFCGAEGLKNV
                     VTLSELRNKDFGKDYGLTITNGPLAGLLARSVVVVDESGKILYTELVPEITQEPNYEN
                     AIAA"
     gene            complement(496..>1099)
                     /locus_tag="PK683_21775"
     CDS             complement(496..>1099)
                     /locus_tag="PK683_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNH11142.1"
                     /translation="LADQAKGPILNPIEMAMPNEKAVIEKLSKIEEYKGLFEKAFPAD
                     KDKLTYNNLASAIAAFERTLITSDRFDDWMSGNWKAMNSEEVNGLALFIQTGCTGCHN
                     GPAFGGNSFRKLGEVKPYDTKDLGRFNVTKSEMDKYVFKVPSLRNIALTAPYFHDGSI
                     KTLDEAVKKMAFHQLGKELNDSEVKSITAFLNTLSDKKRL"
BASE COUNT          268 a          251 c          215 g          365 t
ORIGIN      
        1 aatgcagcaa ttgcattttc gtaattaggt tcctgtgtga tttctggaac aagttctgta
       61 taaagaatct ttcctgattc atctacgact acaactgaac gagcaagcag tcctgccagt
      121 gggccgtttg ttatagtgag tccgtagtct tttccaaagt ctttattcct cagttcactc
      181 agagtgacaa cattcttcag accctcagct ccgcagaatc tgctttgcgc aaacggaaga
      241 tcagcagaaa tacatagtac tgctgtattc tccagcttgc ttgcctgttc attgaacttt
      301 ctcactgatg ttgcacagac tgcagtgtca atgctcggaa aaatattcag aacgagtttt
      361 ttaccgctga atgatttgag agtcgcatcg ctcagatctg ccttggtcag tgtaaaatcc
      421 ggtgcagaat ctcctttctt gggaagatcc cccgatgttg aaatctcgtt tcctttgagt
      481 gtaacttttg ccattttaga gcctcttctt gtctgataat gtgtttaaaa atgctgtgat
      541 tgatttcact tcagaatcat tcagttcttt tcccagctgg tggaatgcca tcttcttcac
      601 agcttcgtcc agagtcttaa tgcttccatc atgaaaatag ggtgcagtga gtgcaatgtt
      661 tcggagggat ggaaccttga acacatattt gtccatttca ctttttgtca cgttgaatct
      721 tcccagatcc tttgtatcat acggttttac ttcaccgagt ttacggaaac tgtttcctcc
      781 gaatgcgggt ccattatgac atccggtaca tccagtctga ataaaaagcg caagtccgtt
      841 cacttcttcc gaattcattg ctttccagtt tccggacatc cagtcatcaa atctgtctga
      901 tgtaatcaga gttctttcaa atgctgcaat tgcagaagca agattattat aagtcagctt
      961 atctttgtcc gcagggaacg ctttttcaaa aagtcccttg tattcctcaa tcttggaaag
     1021 tttttcaatg actgcctttt catttggcat cgccatttca atcggattca ggatcgggcc
     1081 ctttgcctga tcagccaga
//
LOCUS       DAOQGJ010001819         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114792_length_1098_cov_3.324065, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001819 DAOQGJ010000000
VERSION     DAOQGJ010001819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114792_length_1098_cov_3.324065"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PK683_21780"
     CDS             complement(<1..437)
                     /locus_tag="PK683_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH11143.1"
                     /translation="MIEKLIEVSIKKRHLVLIFTGLLCMIGFYNAMHLSVDAVPDVTN
                     VQVSVVTSSPGLSPIEVEQFITYPVELAMNGLPNVEEIRSISRTGVSSVTIIFKDSVD
                     IWFARQLVNERVKQVENEIPDGYGKPELSPVATALGDIYEFVLT"
     gene            complement(437..>1098)
                     /locus_tag="PK683_21785"
     CDS             complement(437..>1098)
                     /locus_tag="PK683_21785"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH11144.1"
                     /translation="EKETSYNTLSVYGLSASEIKNLETGRLNPSELILRSPIGGTVTA
                     RKAVNGQAVITEDNIFVVANLAKLWILLDVYEKDLYSVKLNAEASVYTLGEKPEIVKA
                     IVAHVSDVIDPVKHTAEIRLEVDNHELKLKPGQTVSAKVQGLISESKSRRIMVLPSES
                     VHKIEGKSYVFIAADGAFEAKEVETGEAIDDDVEIKSGISSEVNVVSAGSFVIKSEFL
                     K"
BASE COUNT          281 a          282 c          215 g          320 t
ORIGIN      
        1 gtcagcacaa attcataaat gtccccgagt gcagtcgcaa caggcgaaag ctcaggcttt
       61 ccgtatccgt ccggaatttc attttccacc tgttttaccc tttcattcac aagctgcctt
      121 gcaaaccaga tatcgacaga gtccttgaat ataatggtca cgctgctgac acctgttctg
      181 gaaatggaac ggatctcctc gacattgggc aggccgttca tggcaagttc cactggatat
      241 gtaataaact gctccacttc aatcggtgaa agacctgggg aggaagtgac aacagaaacc
      301 tgaacatttg tcacatcagg cactgcatcc acagaaagat gcattgcatt gtaaaacccg
      361 atcatgcaga gaagtcctgt aaatatcagg acaagatgtc tttttttaat ggagacttct
      421 atcagttttt caatcattac ttcagaaact cgcttttaat tacaaaggaa cctgcactga
      481 caacattcac ttccgaagat ataccggact taatttccac atcatcatca atggcctccc
      541 ctgtttccac ttctttggct tcaaatgctc cgtctgcagc aatgaagaca taggattttc
      601 cttctatttt gtgtacggat tcagaaggaa gaaccatgat ccttctcgac ttgctttccg
      661 aaatcagtcc ctgcacttta gcgctgacag tctgaccagg cttcagtttc agttcatgat
      721 tgtccacttc cagtctgatc tctgcagtgt gcttcacagg atcaatcaca tcgctcacat
      781 gcgctacgat cgccttcaca atctccggtt tttctccaag cgtataaacg ctcgcttccg
      841 cattgagttt tactgaataa agatcctttt cataaacatc cagaagtatc cagagttttg
      901 ccagatttgc cacgacgaat atattgtctt ccgtgatcac tgcctgaccg ttgactgcct
      961 ttctggcagt gactgtcccg cctatcggac tgcgcaggat cagttcgctc ggattgagtc
     1021 tccctgtttc cagattttta atctccgagg cggaaagacc gtagacagaa agtgtattgt
     1081 agctggtttc cttttccg
//
LOCUS       DAOQGJ010001820         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114945_length_1097_cov_4.255278, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001820 DAOQGJ010000000
VERSION     DAOQGJ010001820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114945_length_1097_cov_4.255278"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PK683_21790"
     CDS             <1..938
                     /locus_tag="PK683_21790"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNH11145.1"
                     /translation="KRRTSSMINIKAVKEDIMYYRRKIILALLKTFNGKLSNSDLQNL
                     LFLFAQRQLKPSFDFLPFRSGCHSPQANQDLKTMIKYNMVKEEADDWILADTKNYIQS
                     LNKKDQQDLPSFASEYSDLRGVPLMRYVYKKYPFYAIKSEYAESILSSEEMAAVNSVR
                     PRNAEPALFTVGYEGKSVEQYVTQLIKEDIKILCDIRKNPMSMKFGFSKSQLKGILEG
                     AGIDYVHIPGLGIDSGKRQNLKTQNDYEKLFAEYEKTTLIQNKSDLAELANLFRKNGR
                     IALTCFESDHHSCHRSRTANALHPLLPKNTPLVHL"
BASE COUNT          364 a          203 c          204 g          326 t
ORIGIN      
        1 gaaaaagacg tacctccagt atgataaata taaaggcagt aaaagaggat ataatgtatt
       61 acagaagaaa aattatactt gcattgctca aaactttcaa tggaaaactt tccaattccg
      121 atctgcagaa tcttctgttt ctgtttgctc aaaggcagtt gaaaccctct tttgattttc
      181 tgccgttccg ctccggatgt cattctcctc aggcgaatca ggatttgaaa acaatgataa
      241 aatacaatat ggtcaaagaa gaagcagacg actggattct tgctgataca aagaattata
      301 tacagtctct gaacaaaaaa gatcagcagg atctgccttc ctttgcttct gaatacagtg
      361 atttgcgcgg agttcctttg atgagatatg tatataaaaa atatcctttt tatgcgataa
      421 agagtgaata tgccgagagt attttatctt ctgaggaaat ggcagcggta aactctgttc
      481 gcccaaggaa tgcagaacct gctcttttta cagtcggcta tgaaggtaaa tctgttgaac
      541 agtatgtgac tcaattaata aaagaggata ttaaaatcct gtgtgatatc agaaaaaatc
      601 ctatgagtat gaagttcgga ttttccaaaa gtcagctgaa aggaattctg gaaggtgcag
      661 gaatcgacta tgttcatatt cccggtttgg gaattgattc agggaaacgt cagaatctga
      721 aaacgcagaa tgattatgaa aaactttttg ctgaatatga aaaaacgact ctgatacaga
      781 acaagtccga tttagctgaa ctggcaaatc tgttccggaa aaacggaaga attgctctca
      841 cctgttttga gtcagaccat cattcctgtc acagaagcag aactgcaaat gctcttcacc
      901 cgcttcttcc caaaaatact cccttagttc atctctaagg cataaactgg aaaatccctc
      961 ctgacagagc gaaaagggcc tgtcagataa ttcttacagc acattctgct tttttcttga
     1021 acagtcatat tattttgcta ttatagtaat tttttcaata tcttcatact tatatacctt
     1081 ttcagaaaaa gctaaat
//
LOCUS       DAOQGJ010001821         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114953_length_1097_cov_3.721689, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001821 DAOQGJ010000000
VERSION     DAOQGJ010001821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114953_length_1097_cov_3.721689"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..>1097)
                     /locus_tag="PK683_21795"
     CDS             complement(32..>1097)
                     /locus_tag="PK683_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11146.1"
                     /translation="SYTEKENGIYESSSLSLPNQGFTVRKTENPSGKFRFAGFEYTVS
                     GYEKTEYKSDIESIILYPVDSSEKESFLSLIENLSLLQKDGKKVYLYGSRMLPIADLD
                     EGRKLLNEIQYRKFPAFPFNEIDSSFGNTLIISGKGRYSVPYTELKNSEYYRLTEEFF
                     QTGKNRVFLHSVSGGVSDFWNSFLGMERVLLLGKTREDLLKFLNEGKILMEKNDESSH
                     TFSNAGITVTKSRTSEKISEGSDRAARLFLYRDLMNLFGRKKDIGQNEWKDILEKAEF
                     IKAVSPVTSLIVLETEADYERFGIKKKTGGLGSSSLQDSFLKSKDGTVPEPEDWILFI
                     LSLFCAVWIFRKFSAVSSGR"
BASE COUNT          306 a          242 c          207 g          342 t
ORIGIN      
        1 ggataaaaat gaaaaaaaga ataacctgaa atcatctccc tgaggaaacg gcactgaatt
       61 ttcggaatat ccagacagcg cagaaaagag acagaataaa aagaatccaa tcttcaggtt
      121 caggaactgt tccatccttg ctcttcagaa aactgtcctg aagcgaagaa ctcccgagtc
      181 cgcctgtctt ttttttgatt ccgaaacgtt cataatccgc ctcagtttca agaacgatca
      241 gagaagtcac aggactcact gccttgatga attctgcctt ttccagaatg tccttccatt
      301 cattctgtcc gatgtctttt tttcttccga acagattcat aagatctcgg tacagaaaaa
      361 gccttgctgc cctgtccgat ccctcactga tcttttctga ggttctggat tttgtgacag
      421 tgattcctgc gttcgaaaat gtatgagagg attcatcatt tttttccatt aaaatcttcc
      481 cctcgttgag aaatttcagc aggtcctctc tcgtttttcc cagaagaagg actctttcca
      541 tccctaaaaa tgaattccag aaatcggata ctcctcctga gactgaatgc agaaaaactc
      601 tgttctttcc tgtctgaaag aactcttcag tcagtctgta atattcagaa tttttcagct
      661 cagtgtatgg aacggaatat cttccttttc ctgaaatgat cagagtattt ccgaatgaac
      721 tgtctatttc attaaaaggg aatgcaggga actttctgta ctgaatctca ttcagaagtt
      781 ttcttccctc gtccaggtct gcaatcggga gcatcctgct tccgtaaaga tacacttttt
      841 ttccgtcttt ctgaagcaga gaaagatttt cgatgagaga aagaaaactt tccttttcag
      901 aggaatctac aggatacagt ataatgcttt caatgtcaga tttgtactca gtcttctcat
      961 agccggatac ggtatattcg aagcccgcaa atctgaattt tccggaaggg ttttctgtct
     1021 tcctgactgt aaatccctga ttcggcaggg aaagtgaaga ggattcatat atgccgtttt
     1081 ccttttcagt ataggag
//
LOCUS       DAOQGJ010001822         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_114974_length_1097_cov_3.221689, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001822 DAOQGJ010000000
VERSION     DAOQGJ010001822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114974_length_1097_cov_3.221689"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1097)
                     /locus_tag="PK683_21800"
     CDS             complement(<1..>1097)
                     /locus_tag="PK683_21800"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH11147.1"
                     /translation="LDIAIQCVTALNEIHRRNVIHKDIKPHNIVYNESTGRLQIIDFG
                     SASLLSRENPVLINTGVLEGTLSYISPEQTGRMNRNIDYRTDFYSLGVSFYQLLTGSV
                     PFSSSDSIELIHCHIAVLPVSPTDRKKDIPYPVSDIVIKLLSKAPEERYQSCTGLLMD
                     LEKCRRQFIASGKIESFPVGLDDFSGKFQIPEKLYGRESDINALLHSFERVQINQKSE
                     MILIAGSSGLGKSALIQEIYKPITKSKGYFVLGKFDQFKKDLPYNAFIQAFTELIRQI
                     LTESEESVEDWKKKILSALGQNAAVLTELVSELEILIGIQAEVPELPSMEAQNRFNST
                     VANFVKVFAAAEHPLAVFLDDVQWADSASLK"
BASE COUNT          311 a          236 c          232 g          318 t
ORIGIN      
        1 gtttgagtga ggcagaatcc gcccactgca catcgtccag aaagacggcc aagggatgct
       61 cagctgcggc aaatactttt acaaaattcg caacagtgct gttgaatctg ttctgtgcct
      121 ccatgctcgg aagttcagga acctctgcct gtatgccgat caggatttcc agctcagaaa
      181 caagttctgt aagaaccgct gcattctgtc cgagagccga aagaattttc tttttccagt
      241 cctccacaga ttcttcagac tctgtcagga tctgtcggat cagttcagtg aaagcctgaa
      301 taaaagcatt gtatggaagg tcctttttaa actgatcaaa ttttccaaga acaaagtaac
      361 ccttgctttt ggtaatcggt ttataaatct cctgaattaa tgctgatttt ccgagaccgg
      421 acgaaccggc aatcagtatc atttcgctct tctgattgat ctgaactctc tcaaatgaat
      481 gcagaagggc atttatatct gattcacgtc catacagttt ttcaggaatc tgaaactttc
      541 ctgaaaaatc atcaagaccg acggggaagg attcaatttt accagaagca ataaactgcc
      601 gtctgcattt ttcgagatcc ataagcaggc cggtacagct ctggtatctt tcttcaggag
      661 ccttgctcaa cagcttgatt actatgtcag aaacaggata tggtatatct ttttttctgt
      721 cagtcggtga aaccggcaga actgctatat gacagtggat cagttcaatt gaatcggaag
      781 aactgaatgg aacagacccg gtaagaagct gatagaaaga gacaccaagg gaataaaaat
      841 cagtccggta gtcaatattt ctgttcatcc ttcctgtctg ttcaggagag atgtatgaga
      901 gtgtcccttc caaaactcca gtattgatca gtacgggatt ttccctggac agaagactgg
      961 cacttccaaa atcaatgatc tgcagtctgc cggtggactc attataaact atattatgag
     1021 gtttgatgtc cttgtggatt acatttcttc tgtgaatctc attcagtgca gttacacatt
     1081 gaattgctat gtcaaga
//
LOCUS       DAOQGJ010001823         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115010_length_1097_cov_2.599808, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001823 DAOQGJ010000000
VERSION     DAOQGJ010001823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115010_length_1097_cov_2.599808"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..>1097
                     /locus_tag="PK683_21805"
     CDS             200..>1097
                     /locus_tag="PK683_21805"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNH11148.1"
                     /translation="MSKKWVYLFNELQAAESTVGGEWDRVRALLGGKGANLADITRLS
                     IPVPFGFTVTTEACNAYIAADSHFTDGMWEQELEALHSLEAQTGKKFGSTENPLLVSC
                     RSGAKFSMPGMMDTVLDIGLNDQTVLGLANLTGDDKFAYDSYRRLIQMFGSVVMGISD
                     DAFEEILTETRKTEGVDTDSELSAGALKKIVDKYKIAFKQKAWRDFPQDPIEQIKLAT
                     EAVFKSWNGKRAIDYRNAEKIPHDLGTAVNIVAMVFGNLGNSSGTGVVTTRNISTGER
                     SLEGDYLINAQGEDVVAGIRATK"
BASE COUNT          330 a          237 c          258 g          272 t
ORIGIN      
        1 atatttcaga aagaaactct catagcctgt tccgcaatac tgactttatc ctccgcacaa
       61 gcctctatcc attttgtctc agaaaaacct ttccgcctcc cccgtaaaac ttgaactgac
      121 aaaaatcata attataaccg cttgctattt ttttgaatta tgataaaatc ttctttgcgt
      181 cttttttcag gaggatttta tgagcaaaaa atgggtttat ctctttaacg aacttcaggc
      241 cgcagaaagt actgtgggcg gtgaatggga cagggtcaga gcgcttctgg ggggaaaggg
      301 tgccaatctg gccgacataa ccagactcag cattcctgta ccgttcgggt ttactgtcac
      361 aaccgaagcc tgcaatgcat atatagctgc ggacagtcat tttacagacg ggatgtggga
      421 gcaggaactg gaagctcttc attcactgga agcacagact ggcaaaaagt tcggaagcac
      481 agaaaaccct ctgctcgttt cctgccgttc cggtgcaaaa ttctccatgc cgggcatgat
      541 ggatactgtt ctggacatcg gtctgaatga tcagactgtt ctgggtcttg caaatctcac
      601 cggagatgat aaatttgctt atgattctta cagacgtctg atccagatgt tcgggagcgt
      661 tgtaatggga atctcagacg atgcatttga agaaatcctc actgaaacaa gaaagacgga
      721 aggagtcgat accgattcag aactgagtgc aggtgcactt aagaaaattg tggacaaata
      781 taaaatagca ttcaagcaga aagcctggag agattttcct caggatccga ttgagcagat
      841 caaacttgcc actgaagcag tattcaaaag ctggaacggt aaaagagcaa tcgactacag
      901 aaatgcggaa aaaattcctc atgatctggg aactgcagta aacattgtag cgatggtttt
      961 cggaaacctg ggcaacagtt ccggaacagg tgtcgtgact acaaggaaca tttccacagg
     1021 agaacgatcc ctggaaggag actatctcat caatgcccag ggagaagacg ttgtagcagg
     1081 gattcgcgca accaaac
//
LOCUS       DAOQGJ010001824         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115168_length_1096_cov_3.183477, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001824 DAOQGJ010000000
VERSION     DAOQGJ010001824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115168_length_1096_cov_3.183477"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..>1096)
                     /locus_tag="PK683_21810"
     CDS             complement(1..>1096)
                     /locus_tag="PK683_21810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11149.1"
                     /translation="VENEAAKITVSIDAEKSRTDQKLEFYAENWNLELERIKSRTAKD
                     VDVLVQRLKDIHVEGKEVSDSIRTEFSNGRTQLDIIVKKAEESLNVHIDSIAEEIQSK
                     VKKSQDEVEMLLGRLQKAGINLYEKQESLLSDYGERLYKDLQTKLEKVRFESEELLED
                     IQKAGMNLLEKQEEKIDRLNTTIDDRISRQLTVLLDKGQLQLDQLENRIASYVHEVKT
                     NIEANLKNSKEDSDRQIAAFNSQIQKNFREIEKDNRDFIDSNRAEFEKAKEDFEKFRR
                     NVESEAEKVARLKGLLLSEFQSEESRIKNTLQKLTGRISEIESYAELFTNTEKVIRES
                     ELTVHSMTALLERLKIEGDSWPPFRKWDDK"
BASE COUNT          234 a          267 c          199 g          396 t
ORIGIN      
        1 ctatttgtca tcccactttc tgaagggcgg ccaagaatca ccttctatct tcagcctttc
       61 gaggagcgct gtcattgaat gcacagtcag ttcagattca cggattactt tttccgtgtt
      121 cgtaaagagc tctgcatagg attcgatctc tgaaattctt ccagtgagtt tctgaagtgt
      181 attcttgatt ctggactctt ccgactggaa ttcactcaga agaagacctt tcagtcttgc
      241 cactttttca gcttccgatt ccacatttct tctgaacttc tcaaaatctt cttttgcctt
      301 ctcaaactca gccctgttgg aatcaatgaa atcgcggttg tccttctcta tttcacggaa
      361 atttttctga atctgactgt tgaaagcggc tatctgacgg tcactgtctt ccttggaatt
      421 cttaagattc gcttcaatgt ttgtcttcac ttcatggaca taggaagcaa ttctgttttc
      481 aagctgatcg agctgaagct gtcccttgtc cagaagaaca gtgagctgtc tggatatcct
      541 gtcgtctatc gtagtgttca gtcggtcaat tttttcttcc tgcttttcaa gcagattcat
      601 tccggctttc tgaatatctt ccagcagttc ttccgattca aatctgacct tttccagttt
      661 tgtctgcaga tccttgtaca gtcgctcacc gtaatcagag agaagggact cctgtttctc
      721 ataaagattg atccctgctt tctgaagtct gcccaggagc atctctactt catcctgaga
      781 ttttttgacc ttgctctgaa tctcttcagc tatggaatca atatggacat tcagactttc
      841 ttcggccttt ttcactatga tatcgagctg tgttcttccg ttgctgaatt ctgttcggat
      901 ggaatcagaa acttcctttc cttccacatg aatatccttc agtctctgaa caagcacatc
      961 cacatctttg gctgttctgg acttgattct ttccagttcc agattccagt tttctgcata
     1021 gaattcaagc ttctgatctg ttctggattt ttctgcatca atgctgactg ttattttagc
     1081 agcctcgttt tccact
//
LOCUS       DAOQGJ010001825         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115171_length_1096_cov_3.100865, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001825 DAOQGJ010000000
VERSION     DAOQGJ010001825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115171_length_1096_cov_3.100865"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PK683_21815"
     CDS             complement(<1..889)
                     /locus_tag="PK683_21815"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNH11150.1"
                     /translation="MTDRILDSLNEPQKEAVLHTEGPLLILAGAGSGKTRVITHRIAH
                     LIMNRNVYPFKICAVTFTNKAAAEMRERIIGLLPNSGHMVTMKTFHSLCLGILRKNPD
                     RVGRKSGFTVLDTGLQESLIKTILKDKELDSKEYRPAYFSNAIQRAKDDLISEEEFLS
                     EFPRPDRHTKALHEVYREYEKRKVQNNAFDFGDLIFYSVKLLQSDTEILEYYRDHWHY
                     LLVDEFQDTNHAQYVLTKLLAGERKNVCVVGDDDQSIYSWRGANIENILGFEKDFPGT
                     KIIKLEENYRSTGNIIEAAK"
     gene            complement(889..>1096)
                     /locus_tag="PK683_21820"
     CDS             complement(889..>1096)
                     /locus_tag="PK683_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11151.1"
                     /translation="SFHIEEMSSVTENIKDSTETQSHSIEETNESMKFISREAMDQYE
                     LIEKFTQVLDFLDEAGKLIQSLHT"
BASE COUNT          257 a          236 c          225 g          378 t
ORIGIN      
        1 tctttgcagc ttcgatgatg tttccggtcg atctgtaatt ttcttccagt ttaattattt
       61 ttgtacctgg aaaatccttc tcgaatccca gaatgttttc aatgtttgcg cctctccagg
      121 aatagatgga ctggtcatcg tctcctacaa cgcatacatt ttttctttcc cctgcgagga
      181 gcttggtaag gacatactga gcatgatttg tatcctggaa ttcatctacc agcagataat
      241 gccagtggtc cctgtaatat tccagaattt ctgtgtcgct ctgaagaagc ttcactgaat
      301 aaaaaatcag atccccgaaa tcaaatgcat tattctggac cttccttttc tcatattctc
      361 tgtaaacttc atggagagcc tttgtatgtc tgtcaggtct cggaaattcg gaaagaaatt
      421 cctcttccga aatcaggtcg tccttggctc tctggattgc atttgaaaaa taggcaggtc
      481 tgtattcctt ggaatcgagt tccttgtcct tgagtattgt cttgatcaga gattcctgaa
      541 gtcctgtgtc aagtacagta aagccgcttt tccgtccgac cctgtccggg ttttttctga
      601 ggatgcccag gcagagagaa tgaaatgtct tcattgtaac catatgtccg ctgttcggaa
      661 gaagtccaat gatcctttct ctcatttcgg ccgccgcttt gttggtaaat gtaacagcac
      721 atattttaaa aggatataca tttctgttca ttatcagatg tgcaatcctg tgagtgatga
      781 ctctggtctt tcctgatcct gcacctgcga ggatcagaag agggccttct gtatggagaa
      841 cagcctcttt ctgaggttca ttgagtgaat cgagaattct gtctgtcatt atgtatggag
      901 gctttgaatc agttttcctg cttcatcgag aaagtccaga acctgtgtga atttttcgat
      961 cagttcgtac tgatccattg cttctctgga aataaatttc atactttcat ttgtctcttc
     1021 tatggaatgg ctctgagtct ctgtggaatc cttgatgttt tccgttacag aactcatttc
     1081 ttctatatgg aatgac
//
LOCUS       DAOQGJ010001826         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115173_length_1096_cov_3.048031, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001826 DAOQGJ010000000
VERSION     DAOQGJ010001826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115173_length_1096_cov_3.048031"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..489)
                     /locus_tag="PK683_21825"
     CDS             complement(79..489)
                     /locus_tag="PK683_21825"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNH11152.1"
                     /translation="MENLKDTVKPLYTSCFSVDKNADPSSIMSELLADDFQSINAGET
                     KSKTQLIGQVQFLWKLIPDLKFDIQEMIQDGNKVIVRCFATGTPKGEFLGMNLDGTKS
                     FRIMSIDIHTLEGGKVKSVHHLEEWTTAIRQLKS"
     gene            complement(575..>1096)
                     /locus_tag="PK683_21830"
     CDS             complement(575..>1096)
                     /locus_tag="PK683_21830"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH11153.1"
                     /translation="KNAAPVIRPGKKSTVRLSADAENIYNEYHFGEDAGKELMMQCLL
                     KIFLIRLERCRTLKSTEQLNPEYAERFRIFLNLLERNCTKIREAETYSKMMNCSYKHM
                     NSICIQAYGTSMKKIIDAHTALEMKRRLAVSDVSVNELTELFHFDEPTNLVKFFKKQT
                     GITPARFRRNLML"
BASE COUNT          286 a          232 c          213 g          365 t
ORIGIN      
        1 agacagaatc agcacaacga ttccgctcag aacattgatc cagactggaa ttttgtctct
       61 tttcatgatg gtttatcctc agcttttgag ctgtctgatt gcggtagtcc attcttccag
      121 atgatgcact gattttactt tcccgccttc cagtgtatga atatcaatgc tcataattcg
      181 gaaagacttt gtaccatcca gattcattcc gagaaactca cccttgggtg ttccagtggc
      241 gaaacatctt acaatcacct tgtttccgtc ctgaatcatt tcctgaatgt caaatttcag
      301 atcgggtatg agtttccaga gaaactgcac ctgtccgatc agctgtgtct tggattttgt
      361 ctctcctgca ttgatggact gaaaatcatc tgcaagcagt tcgctcataa ttgaagaagg
      421 gtctgcattc ttatccacac tgaaacatga tgtatacagg ggttttactg tatctttaag
      481 gttttccatt tgctgctcct gaatataata tgtcattttt tcaggaaaat gaaaaggtca
      541 gagtaagaaa aaaaatggta aaaatcgaac ctgatcagag catcaaattt ctccgaaatc
      601 tggccggagt tatgcctgtc tgttttttga agaatttcac cagatttgtc ggttcatcaa
      661 aatgaaaaag ttcagtcagc tcatttacag aaacatcgga cacggcaagt cttctcttca
      721 tttccaaggc tgtatgcgca tctatgattt ttttcatgga tgttccatat gcctgaatgc
      781 agatgctgtt catatgcttg tagctgcagt tcatcatttt tgagtatgtt tccgcttctc
      841 tgattttggt acagtttctt tctagaagat tcagaaaaat cctgaaccgt tctgcatatt
      901 ccggattgag ctgttctgtg cttttcagag ttctgcacct ttcaagacgg atgagaaata
      961 tcttcagaag acactgcatc atcagttcct tgccggcatc ttccccgaaa tgatattcgt
     1021 tataaatgtt ttccgcatcg gcagaaagcc tgactgtcga tttttttcct ggacgaatga
     1081 caggagcagc attttt
//
LOCUS       DAOQGJ010001827         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115422_length_1095_cov_2.584615, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001827 DAOQGJ010000000
VERSION     DAOQGJ010001827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115422_length_1095_cov_2.584615"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK683_21835"
     CDS             <1..387
                     /locus_tag="PK683_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="band 7 protein"
                     /protein_id="HNH11154.1"
                     /translation="NIEIAVQEKQRQLEEASMNTELELESKRAELEKMKLQASIDLEE
                     QRKKLIQSESSNLIESSRAKGEAMKLELSAFAGLSQELIEALTANQLNSSQIISRAMR
                     DMAKNAEKIGNLNISPDLLNSLMQNQ"
     gene            402..>1095
                     /locus_tag="PK683_21840"
     CDS             402..>1095
                     /locus_tag="PK683_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ kinase"
                     /protein_id="HNH11155.1"
                     /translation="MEIEKIVLVVRPTRLEENLRRHNTKQQAKFFIQSRGQSFEDYEK
                     EDSNYHNSRDDISQYIPKGIKLQIIDRDFLPNFVFGPNDAVIALGQDGLVVNIAKYLN
                     GQPIIAVNPDPERFDGILLPFQTQDIGNAIQSVLKDTAELKEITMAKASLNDGQELFA
                     FNDFFIGPKTHTSARYMIRYRDYAERQSSSGVIISTPAGSTGWLSSVFNMAEGMMGTE
                     SGRKGGRRKNRAE"
BASE COUNT          382 a          224 c          251 g          238 t
ORIGIN      
        1 aacatcgaga tagcagttca ggaaaaacag agacagctcg aagaggcttc catgaacacg
       61 gaactcgaac tggaatccaa aagagcagaa ctcgaaaaaa tgaaacttca ggcatccatt
      121 gatctggaag agcagcgaaa aaaactcata cagagtgaat cgtccaacct gattgaatct
      181 tcaagagcaa aaggggaggc aatgaaactt gaactttctg ccttcgcagg tctcagccag
      241 gaactcattg aggcgcttac tgcaaatcag ctgaattctt ctcaaatcat cagcagggca
      301 atgagagata tggcgaaaaa tgcagaaaag atcggcaatc tcaacatatc accggatctg
      361 ctgaattcac tgatgcagaa tcagtaaaga acaggaagag attggaaata gaaaaaattg
      421 tactagttgt ccgtccgacg cggcttgaag aaaatctgcg tcggcacaat acaaaacagc
      481 aggcaaaatt tttcattcag tcaagaggcc agtcattcga agactatgaa aaggaagact
      541 cgaattatca taattcgaga gatgatatat cccagtatat cccaaaagga ataaaacttc
      601 agatcataga cagagacttc cttcctaatt ttgtatttgg accgaatgat gctgtaattg
      661 cactcggcca ggacggactt gtagtgaaca ttgcaaagta cctgaacgga cagccgatca
      721 ttgcagtcaa ccctgatcca gaaagattcg acggaatcct tcttccgttc cagacacagg
      781 acatcggaaa tgcaattcag tccgtactga aggatacagc agaattgaag gaaatcacaa
      841 tggcgaaggc aagtctgaat gacggtcagg aactgtttgc atttaatgat tttttcatcg
      901 gcccaaaaac tcataccagt gcaagatata tgatccggta cagggattat gctgaaagac
      961 agtcttcaag cggagtcatc atttccactc ctgccggctc taccggatgg ctaagttccg
     1021 tttttaatat ggcggaagga atgatgggga ctgaatcagg aagaaaagga ggcagaagaa
     1081 agaatcgagc agaac
//
LOCUS       DAOQGJ010001828         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115624_length_1094_cov_2.771896, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001828 DAOQGJ010000000
VERSION     DAOQGJ010001828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115624_length_1094_cov_2.771896"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PK683_21845"
     CDS             complement(<1..1045)
                     /locus_tag="PK683_21845"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNH11156.1"
                     /translation="MKLETAFTEERNIPDHWTGISEMDRLLTDSKCGVIQKASEISGI
                     GHRVVHGGETFTRPTRITDSVIKAIEENIPLAPLHNPANLSGIYAAEKFFKGVPNIAV
                     FDTSFHQSIPEFAFRYALPSEYYSRHRIRRYGFHGTSHQYIVKKTAEYLKKDINSLNI
                     ISLHLGNGASICAVKNGKSVDTSMGMTPLEGLIMGTRTGDIDPAVHFYLADHAGLSFK
                     EIDNVFNKQSGLKGICGSNDMRDILQKMNDGDRNAKLAFEMYCYRIRKYIGAYIFALE
                     GKTDAVVFTAGVGENSSDVRMKVMEGLQAFGLGLDNEKNSKREPGIRDLSIVQNTVRV
                     LVVPTNEELEIAEQ"
BASE COUNT          248 a          243 c          224 g          379 t
ORIGIN      
        1 actgttctgc tatttccagt tcttcatttg tcgggacaac caggacacgt actgtattct
       61 gtacaatgct cagatctctg attccaggtt ctcttttgga gtttttttca ttgtcgagtc
      121 ctagtccaaa tgcctgaagt ccttccatta ctttcattct tacatcagat gaattctcgc
      181 ccacgcctgc tgtgaatacg actgcatctg tttttccttc cagtgcaaat atataagctc
      241 ctatatattt tctgattctg tagcagtaca tttcaaatgc aagtttggcg ttcctgtctc
      301 catcgttcat tttctgcaga atgtctctca tatcgttcga gccgcagatt cctttcagac
      361 cgctctgttt gttgaacaca ttgtctattt ccttgaaaga gagccctgca tgatctgcaa
      421 gataaaaatg cacagcagga tcgatgtcgc ccgttcttgt gcccataatc agtccctcca
      481 aaggagtcat tcccatggag gtgtcaacgc tttttccgtt ttttacagca cagatgcttg
      541 ctccgtttcc cagatgtagg gatatgatat ttaatgaatt tatatccttt tttaagtatt
      601 ctgctgtctt ttttacaata tactgatgag aggttccgtg aaatccgtat cttctgattc
      661 tatgtctgct gtagtattct gacggcagtg catatctgaa ggcaaattca ggaatggact
      721 gatgaaatga agtgtcaaac actgcgatgt tcggaacacc tttgaagaat ttttcagcgg
      781 catagatgcc agagagattt gccggattgt gaaggggtgc gagaggaata ttttcttcta
      841 ttgctttgat gaccgagtca gtgattctgg tcggtctggt aaatgtttct ccgccgtgca
      901 caactctgtg accgattccg ctgatctctg aagccttttg aatgactccg cattttgagt
      961 cagtgaggag cctgtccatt tcgctgatcc ctgtccagtg gtcaggaata ttcctttctt
     1021 cagtgaaagc tgtttccagt ttcactgatt tataagtatg cttagctgtt ttctcaccga
     1081 ttcgttccat aaga
//
LOCUS       DAOQGJ010001829         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115684_length_1094_cov_2.131858, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001829 DAOQGJ010000000
VERSION     DAOQGJ010001829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115684_length_1094_cov_2.131858"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PK683_21850"
     CDS             complement(<1..434)
                     /locus_tag="PK683_21850"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNH11157.1"
                     /translation="MENLTGSLALFWPQLILGAVLGALLSSFGIILLLRNMSFYSITL
                     AQITTFGFAAALFFRLPAEIFTPVFTSVIMLLSVYLTAKTSYRTESVLAILFVSFGAM
                     SQALLAFGGNVQNHILSAYFGDILTSQVRLDSPVIFITLGCAA"
     gene            complement(436..1071)
                     /locus_tag="PK683_21855"
     CDS             complement(436..1071)
                     /locus_tag="PK683_21855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH11158.1"
                     /translation="MTLLVSAKNLTIGYSKNYPVISGINLDIRTGDRIAVTGGNGSGK
                     TTLIKTISGLLEKISGELFISKKKRVSMVPQIKNVRLHFPVTVKDAILMPEENFFWKN
                     RKFSETEMVLLEKLGLNGLLKKQLKECSGGQIQKTLLARSVISRPSLIILDEPLDALD
                     ISSQKTVYSVLEDADIMKDTAVITITHNIYSENMSFYKKIYEASEGLLREK"
BASE COUNT          301 a          227 c          233 g          333 t
ORIGIN      
        1 gctgcacagc cgagagtaat gaagattacc ggcgagtcca gtctgacctg acttgtgagt
       61 atgtctccga agtatgcaga cagtatatga ttctgcacat tcccgccgaa cgcaagaagt
      121 gcctgcgaca ttgccccaaa gcttacaaaa agtattgcaa ggaccgattc tgtcctgtaa
      181 gaagtctttg cagtaagata cactgaaagc agcataatca ctgatgtaaa cacaggagtg
      241 aaaatttctg caggaagcct gaaaaaaagc gctgctgcaa atccgaaagt tgtaatctgt
      301 gcaagtgtaa tgctgtaaaa ggacatattt ctcaggagaa gaattattcc gaatgaagaa
      361 agaagtgcac ccaaaactgc accgagaatc agctgcggcc agaaaagggc aagactgcca
      421 gtcagatttt ccatatcatt tctccctgag cagtccttcg gatgcttcat atattttttt
      481 ataaaaactc atattttcac tgtaaatatt gtgagttata gtgatgactg ctgtgtcttt
      541 cattatatct gcatcttcca gaacggaata tactgttttc tgagacgata tgtcaagggc
      601 gtcgaggggc tcatccagaa tgatcagcga gggtcttgaa atcacagatc ttgccaggag
      661 agtcttctga atctgtccgc cgctgcattc tttcagctgt tttttcagca gtccgttcag
      721 tcccagtttt tcgagaagaa ccatttctgt ttctgagaat tttctgtttt tccagaaaaa
      781 attttcttca ggcataagta ttgcgtcttt cactgttacc ggaaaatgca gacggacatt
      841 tttgatctgc ggaaccattg atactctttt tttctttgag atgaaaagtt cacctgagat
      901 tttttcaaga agcccggata tggttttgat cagagtagtc tttcctgatc cgtttccccc
      961 ggtcactgca attctgtcgc cagtccgtat atccagattt atgccggaaa tcactggata
     1021 gtttttggaa tatcctattg taagattctt cgcggaaacc agaagggtca ttgttttttc
     1081 ctggtttttt caat
//
LOCUS       DAOQGJ010001830         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115771_length_1093_cov_3.678227, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001830 DAOQGJ010000000
VERSION     DAOQGJ010001830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115771_length_1093_cov_3.678227"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PK683_21860"
     CDS             <1..490
                     /locus_tag="PK683_21860"
                     /inference="COORDINATES: protein motif:HMM:NF024580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4007 family protein"
                     /protein_id="HNH11159.1"
                     /translation="CRIWLDANFQKSYSDRSLENDFSVMTAMYCSEEKKDLFYPSPFL
                     SCEILKYNPHLKRYSRNKDLKIDIHIILYCILLFRNRYFRDAGVLDLDHLRQIPESPL
                     RVFALETDRIFELSEEIVPKIAKKVSFSRTAGIKTVSFSNLEETELLNEIYRQERVLS
                     YI"
     gene            495..>1093
                     /locus_tag="PK683_21865"
     CDS             495..>1093
                     /locus_tag="PK683_21865"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH11160.1"
                     /translation="MKYFDYNSTSPVHPKIREKISEWLEEYGNPSSQHGIGRKASARL
                     NEARQSILSALNLESYHLTFTSGGTESNHLAFQFLKTSVPKRKKIFLSSIEHPCIIKQ
                     KDVLSRIGYNIIIIPAEKNGQVNLNFLEDHLDEDTALCAVMGANNETGVIQDISKISE
                     LCREYEIHYHCDVSQIPGKARIDLEKADADSYTFSSHKV"
BASE COUNT          349 a          211 c          222 g          311 t
ORIGIN      
        1 ctgcaggatt tggctggatg caaattttca gaaatcgtat tcagacagat ctcttgaaaa
       61 cgatttttcc gtgatgaccg cgatgtactg ttcagaagaa aaaaaggatc ttttttatcc
      121 ttcgcccttc ctgtcctgtg aaatcctgaa atacaaccct catttgaaac ggtattcccg
      181 aaacaaagat ttgaaaattg atatacatat tatattatac tgcattttgc ttttcagaaa
      241 cagatatttc agagatgcgg gcgttctgga tctggatcat ctccgtcaga ttccggaatc
      301 ccctctccgc gtatttgccc tggaaaccga cagaatcttt gaactttccg aagagattgt
      361 tccaaaaatt gcaaagaagg tttcattttc cagaacagca gggataaaga cagtcagttt
      421 ttccaatctg gaagaaacag aactgttgaa tgaaatttac agacaggaaa gagtgctgag
      481 ttatatctga gctgatgaaa tactttgatt acaattccac atctcctgtt catcccaaaa
      541 tcagggagaa gatttctgag tggctggaag aatacggaaa tccttcgtct cagcacggaa
      601 tcgggagaaa ggcatctgca aggctgaatg aggcaagaca gtctatcctt tcagcactta
      661 atctggaatc ttatcatctt acattcacat caggaggtac agagtcaaat catctggcgt
      721 ttcagtttct gaaaacctcc gttccaaaaa ggaaaaaaat ctttctttcc tcaattgaac
      781 atccctgcat aatcaagcag aaggatgttc tttcaagaat aggatataat ataattataa
      841 ttcctgcaga gaaaaacgga caggtgaatc tgaactttct tgaagatcat ctggatgagg
      901 atactgcgct atgtgctgtg atgggagcga acaatgagac aggtgtcatt caggatattt
      961 caaagatctc ggaactttgc agagaatatg aaatacatta tcactgtgat gtatcccaga
     1021 ttcccggaaa agcacggatt gatttagaaa aggcagatgc agacagttat actttttctt
     1081 cgcacaaggt ata
//
LOCUS       DAOQGJ010001831         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115977_length_1092_cov_3.322083, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001831 DAOQGJ010000000
VERSION     DAOQGJ010001831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115977_length_1092_cov_3.322083"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PK683_21870"
     CDS             <1..1051
                     /locus_tag="PK683_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH11161.1"
                     /translation="FFPLLWALIPLFNRMGWHLFARLFYSGTVMLCVTLNSLQAGRES
                     DNHLFLLVASLFAFYIYPPYQMRYIKTVSISALVLFIGIEIYLTDKGPILQAPPEFFR
                     MARLLTLVSLCILVYILTLYNYNVLHKSQDLLEIEHQKSETLLLNILPPAIADRLKAS
                     TEVIADRTNEATILFADVVGFTVLSQTMEPEKLVALLNDLFTEFDRIIQAYGLEKIKT
                     IGDAYMVAGGIPMPRTDHCEAVALCALEMQSVMRKGISSELKDFRIRIGIHTGPVVAG
                     VIGKSKFIYDLWGDSVNTASRMESHGVEDKIHVSKEVYLKLQDRFEFEDRREIQVKGK
                     GIMETYFLVRDKFSI"
BASE COUNT          292 a          232 c          248 g          320 t
ORIGIN      
        1 gtttttccct ctgctctggg cactgattcc cctattcaac cgtatgggat ggcatctttt
       61 tgcgagactt ttctattcag ggactgtaat gctctgtgtc acattgaact cacttcaggc
      121 aggccgggaa tcggacaatc atctgtttct tcttgtcgcc tcactttttg ccttttatat
      181 atatcctccg taccagatga gatatataaa aacagtctca atttcagctc tggtgctgtt
      241 catcggaatc gaaatttatc tgacggacaa agggccgatt ctccaggcac ctcccgaatt
      301 tttccgaatg gcaaggcttt tgactcttgt ttctctctgc atactcgtgt atattctgac
      361 cctttataat tataatgtac ttcacaaatc gcaggatctt ctcgagattg agcatcagaa
      421 atcggagacc cttcttctga acatcctccc tcccgcaata gcggacagac tgaaggcaag
      481 cactgaggtc atcgccgatc ggaccaatga ggcaacgatc ctgtttgcag atgttgtcgg
      541 ctttactgtt ctttctcaga caatggaacc ggaaaaactt gtcgctcttc tgaacgatct
      601 ttttactgaa tttgaccgga ttattcaggc ctacggactt gaaaagatca agacaatagg
      661 agatgcctat atggttgcag ggggaattcc aatgcctcgg accgatcact gtgaggcagt
      721 tgctctctgt gcactggaaa tgcagtctgt tatgagaaag ggcatttcca gtgagctgaa
      781 agatttcaga attcgaatcg gaattcatac aggacctgta gtcgcaggtg tcatagggaa
      841 aagtaaattt atttatgatc tttggggaga cagcgtgaac acagcaagcc gaatggaatc
      901 tcacggtgtt gaggacaaga ttcacgtttc caaggaagtg tatctgaaac ttcaagacag
      961 gttcgagttt gaggacagaa gggagattca ggtaaaagga aagggtatta tggaaaccta
     1021 ttttcttgtt agagacaagt tttccatcta gttctcagat ttggaaacac tgaaaatcgg
     1081 ttcagtgtaa aa
//
LOCUS       DAOQGJ010001832         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_115995_length_1092_cov_2.853423, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001832 DAOQGJ010000000
VERSION     DAOQGJ010001832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115995_length_1092_cov_2.853423"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..>1092)
                     /locus_tag="PK683_21875"
     CDS             complement(78..>1092)
                     /locus_tag="PK683_21875"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH11162.1"
                     /translation="YAVNYDADEILKFLIRSRADVNVQDENGKTALIYSVEEGKTDIT
                     DILLEADADPEIKDKTGNTALSAASRKKDIEFKKNEYSDETKAWSKTVEMLIDSVRKK
                     SIPKIPVKVLRSAEKEKKVRRSPKNIFEYLLILPPEFTQLEKDELNYDYGLRLKYYQE
                     ILFNRECESKKPPDCIEQGFENLDLQNGYLRYREAGLEEYIEMSLFKKSNKTYIIGVT
                     KYFGGDSGRFNTYFLTYKNRKWTDITEKILPELSYKLFLNPKDHKLIGKEQKNDPRGF
                     ISLPRSGTDVFYNADTANSCEGDMGDDTVSKRKNCKEILKKAVRNSVKLKWNPDKGIF
                     ALK"
BASE COUNT          288 a          231 c          171 g          402 t
ORIGIN      
        1 tagttaagca tttcatatta ctttttaaat cctttgtttg aaatttcatt ttttcctcca
       61 taactttatt ataaatctca ttttaatgca aatattccct tgtccggatt ccatttcagt
      121 ttcacggaat ttcgaactgc tttttttaat atctctttgc aattcttcct cttagatact
      181 gtatcatcac ccatatctcc ttcgcacgag tttgcagtat ctgcattgta gaaaacatct
      241 gttccggatc gggggaggga aataaaccca cggggatcgt ttttctgttc ttttccaatc
      301 agtttatgat cttttggatt caagaataat ttataactca gctccggaag aattttctca
      361 gtaatgtcag tccatttccg atttttgtac gtcagaaaat aagtattgaa tctgccggag
      421 tctcctccga aatactttgt aactccgata atataagtct tattagattt tttgaacaga
      481 gacatttcga tgtattcttc aagaccggcc tcccgataac gaagatagcc gttctgcaga
      541 tcaaggttct caaaaccctg ttcaatgcag tccggaggtt ttttactttc acattctctg
      601 ttaaataaga tttcctggta atatttcagt ctcaatccat aatcataatt caactcatcc
      661 ttttccaact gagtaaattc tggagggagg atcaaaagat actcgaaaat atttttggga
      721 gaacggcgga ctttcttttc cttctctgcc gatctgagca cctttactgg aatttttgga
      781 attgattttt tcctgacaga atcaatcagc atttcaaccg ttttagacca ggcttttgtc
      841 tcatccgaat attcattttt cttgaattca atatctttct ttcggcttgc agcagacaga
      901 gctgtgtttc ctgttttatc cttgatttcc ggatcagcat ctgcttcgag aagaatatcc
      961 gttatgtcag ttttgccttc ttcaacagaa tatataagag cagtttttcc gttctcatcc
     1021 tgaacattca catctgccct ggaccgaatc agaaatttca gaatttcatc agcatcataa
     1081 ttcactgcat at
//
LOCUS       DAOQGJ010001833         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116175_length_1091_cov_3.479730, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001833 DAOQGJ010000000
VERSION     DAOQGJ010001833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116175_length_1091_cov_3.479730"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK683_21880"
     CDS             <1..227
                     /locus_tag="PK683_21880"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNH11163.1"
                     /translation="MNSVHTTVTRRIENAKPPVFEYEKKDDKTLIMKYRSERGMIDFM
                     IGLIKGVGKYYNEKLTILKMSETEVKITFS"
     gene            complement(310..696)
                     /locus_tag="PK683_21885"
     CDS             complement(310..696)
                     /locus_tag="PK683_21885"
                     /inference="COORDINATES: protein motif:HMM:NF015678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HNH11164.1"
                     /translation="MNQVVNFILSLLFGVFLSAVHLVLFGIFAVTVVGKPFGDSLFEL
                     SKFFIWSADKWLTRDKDMDDSAALTVLAVLYLPVGLVLALAVLTFGVLSFCTIAGYTF
                     GYKYIKILPFVVWPLRTRCVSEEYVG"
BASE COUNT          401 a          204 c          222 g          264 t
ORIGIN      
        1 gtatgaattc agttcatacg acagtgaccc gcagaattga aaatgcaaaa ccgcccgttt
       61 tcgaatatga gaaaaaggat gataagactc tgattatgaa atacagatcc gagagaggaa
      121 tgattgattt tatgatcgga cttataaagg gagtcggaaa atactacaat gaaaaactga
      181 caatactgaa aatgagcgag actgaagtaa aaatcacatt cagctgaaac tggcggcgga
      241 gcgaaataaa aatccagtct gtgagaatcc cttgcattag agcagggatt cttttttcag
      301 tagaataatt cagcctacat attcctcact gacgcatctg gttctcagcg gccatactac
      361 aaatggaaga attttaatgt atttatatcc aaatgtatat ccggctatcg tgcagaatga
      421 aagcaccccg aacgtcagaa ctgcaagagc aagaacaagc cccacaggaa ggtagagaac
      481 tgcaagcact gtcagagctg cgctgtcatc catatccttg tctctggtaa gccatttgtc
      541 ggcagaccag ataaaaaact tcgacagttc aaacagagaa tcaccgaaag gtttgcccac
      601 aactgtaact gcaaagatac cgaagagcac gaggtggaca gcagaaagaa acactccaaa
      661 cagaagagaa agaatgaaat tgacaacttg gttcatttga attcctgtat tttccaaaac
      721 ttatgtactg aaaaaagaat tacttttttt acagtaaaaa cagatagcac taaaccagat
      781 cgaacacttt tgtcaatctg atttcagcag aaacttcctg aattgcatta aacagattca
      841 tttgaaaatt cagtatcttt ttgcagtcaa atcgcacagt gagagagggg ttttacagca
      901 gagccaacgg aaataaccct gaatgaaaaa atgtacgtct gtcataaaag ctgcagaata
      961 gaaattgaaa aatctgaaca gcggaaataa taggcaaagt tatgtttgga gaaatgcttc
     1021 ttaaagtctc actgtttact cttctgaatc ttcagggaat cagctctgaa agagcagaga
     1081 aggaaacgaa t
//
LOCUS       DAOQGJ010001834         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116186_length_1091_cov_3.230695, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001834 DAOQGJ010000000
VERSION     DAOQGJ010001834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116186_length_1091_cov_3.230695"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1091
                     /locus_tag="PK683_21890"
     CDS             <1..>1091
                     /locus_tag="PK683_21890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNH11165.1"
                     /translation="AGKRLIFLPDERKAILGFSIDISEQMLAEKELHRQKLILDNVLN
                     TDPNLIFLKDQEGRFLLVNRAVETAFEMSAEEIINQSNYAVHQVHEEVDYYAKIDRQV
                     LETMNAVSVVESFTKSNGEKIWYRTVKTPLTMSDGTVHILAVSTDINEIVIKEQELIK
                     AKEAAENLARIKSDFLSTMSHEIRTPLNALVGLTHIMLNEEYLPSQQENLHILSFSAE
                     HLLTLVNDILDFSKIEAGKVELESVEMNPALHLKKILQTLNFKAQDSANELNLFLDEK
                     LPETVISDPFRLSQIFTNLISNSIKFTNGGKIDISAYVTELSGCKAAVRFQVSDSGIG
                     ISADKIETVFGEFSQADSSTSRRFGGTGL"
BASE COUNT          343 a          219 c          245 g          284 t
ORIGIN      
        1 tggcaggaaa gagactcatt tttcttccgg acgaaagaaa agcgattctc gggttttcga
       61 ttgatatatc cgaacagatg ctggctgaaa aagaacttca tcggcagaag ctgattctgg
      121 acaatgtact gaataccgat cccaatctga tatttctgaa agaccaggaa ggccgctttc
      181 ttcttgtaaa cagagctgtt gaaactgcat ttgaaatgtc tgcagaggaa attatcaatc
      241 agagcaacta tgccgttcat caggttcatg aagaagttga ttattatgct aagatagaca
      301 gacaggtatt ggaaacaatg aatgctgtat cggtagtgga aagtttcacc aagtccaacg
      361 gtgaaaaaat ctggtacaga actgtaaaga cccccttaac catgtctgac ggcactgttc
      421 acatcctggc ggtcagcaca gatattaatg aaattgtaat taaggaacag gaactgataa
      481 aggccaagga ggcagcggaa aatctggcca gaatcaaatc cgactttctt tccacgatga
      541 gtcatgaaat caggacacct ctgaatgcgc ttgtgggtct cactcatatt atgctgaatg
      601 aggaatacct gccctctcag caggagaatc tgcatatact ttcattttca gcagaacatc
      661 ttctcactct tgtaaatgat atattagatt tcagtaaaat tgaagcagga aaagtggagc
      721 tggaatccgt tgagatgaat ccggcactgc atctgaaaaa aatcttacag acactgaact
      781 tcaaagcgca ggattctgca aatgaactga atctttttct ggatgaaaaa cttccggaaa
      841 cagtgatcag tgatccattc cggctttcgc agatatttac gaatctgatt tccaattcca
      901 taaaatttac aaatggagga aagatagata tttctgcata cgtgactgaa ctttccggct
      961 gtaaagcagc agtgcgtttt caggtcagtg acagcgggat cggaatttct gccgacaaga
     1021 tcgaaaccgt tttcggtgaa ttcagtcagg cagacagctc caccagccgc aggttcggag
     1081 ggacaggtct t
//
LOCUS       DAOQGJ010001835         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116253_length_1091_cov_2.328185, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001835 DAOQGJ010000000
VERSION     DAOQGJ010001835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116253_length_1091_cov_2.328185"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PK683_21895"
     CDS             <1..513
                     /locus_tag="PK683_21895"
                     /inference="COORDINATES: protein motif:HMM:NF021467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HNH11166.1"
                     /translation="LLNNPNIPSGNSVDVNQGASGANATEKRKFKNKTAQEIADDIIS
                     GIEAVEKDGIFKAKIAVFPQSAKFRFMKLISSSTSMTLMTWLKQEAGISIEMIFTNEM
                     ASSYNKLGSDAFLIMDNDSEIAELALPRDITLGEPVYDLLRNSEQAVTERTAGCILRH
                     PSAVYIGKGI"
     gene            528..905
                     /locus_tag="PK683_21900"
     CDS             528..905
                     /locus_tag="PK683_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11167.1"
                     /translation="MEISLEEFRKSIPAVTLDDAAVTKYLADAARAVTRDGVISDHKE
                     FDELQKFYALGLMQKDRVSGIQSAYAVGKNPEGISSLSVAGVSLGFQSPSASSFTSEG
                     KTGYFLDYDVLLKKVRGLKGRIA"
BASE COUNT          333 a          220 c          275 g          263 t
ORIGIN      
        1 ctgctcaata atccgaatat tccttcaggc aactcggtag acgtgaatca gggagcatcc
       61 ggagccaatg cgacagagaa gcggaagttc aaaaacaaga ctgcacagga aattgcagat
      121 gatatcatct ccggtattga ggccgtagaa aaggacggga tcttcaaagc taaaattgca
      181 gtattccctc agtctgccaa attccgtttc atgaagctta tcagttcaag cacttcaatg
      241 accctgatga catggctgaa gcaggaagcc gggatcagca ttgaaatgat tttcacgaat
      301 gagatggcat catcctacaa taaactggga tcagatgcat ttctcatcat ggacaatgat
      361 tctgaaatcg cagagctcgc tcttccaagg gatatcactc tcggtgagcc tgtctatgat
      421 ctgcttcgca attcagagca ggcagttact gagagaactg caggctgcat tctcagacat
      481 ccgtctgcgg tgtatatcgg aaaagggata taaggagggt ctgcatcatg gaaatatcgt
      541 tggaggaatt caggaaaagc attccggcag tgactctcga tgatgccgca gtcactaaat
      601 atctcgctga tgcggccaga gcagtgacgc gtgacggagt aatttcagat cataaggaat
      661 ttgatgaact tcagaaattc tatgctctcg gtctgatgca gaaggacaga gtctcaggca
      721 tccagtccgc atatgctgtc ggaaaaaatc cggaaggaat cagcagtctc agcgtggcag
      781 gggtgagtct gggtttccag agtccgtctg catcctcgtt cacatctgaa ggaaagacag
      841 gttatttcct cgactatgat gttctgctga aaaaggtgag aggtctgaaa ggcagaattg
      901 cctgaaagac ttttcattat aaaaaatttc aaaggagaaa gtttatgccg caggcattga
      961 aaattgcaat cactgcagac aagatagatg atgtcacgaa tgcagtcctg atgaaggatc
     1021 tggtaaaggt caatgaagtc agtcccagag tttatgggga tgacggaaaa gaaatgaaaa
     1081 ttgatatcat c
//
LOCUS       DAOQGJ010001836         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116517_length_1089_cov_6.753385, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001836 DAOQGJ010000000
VERSION     DAOQGJ010001836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116517_length_1089_cov_6.753385"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..944
                     /locus_tag="PK683_21905"
     CDS             261..944
                     /locus_tag="PK683_21905"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNH11168.1"
                     /translation="MTALKSIYENEPAKLYELEKVFKSVKAGRKNSIAIKADEVYSEE
                     TVNVMIEELSSRQNLSKKNNTDPAKFQKLGLIVWTLFNTGLRISELTGIRNQDVHLNG
                     KATIKVLGKGSKERTVYLPKDAVMEIREVFESADSEYLFCNMRQGRLNRENLTNELSK
                     ALKKDYPDFHPHKLRHSFATKEIISKKKSVKAVSKYLGHSSTAITQDLYVHDEIVFDD
                     LEFTERKRA"
BASE COUNT          383 a          207 c          250 g          249 t
ORIGIN      
        1 acataatgta aattttgtag tttacaaaat agaggggaaa atgatacggt catttcatgg
       61 aactctcaaa gagacagacg gactccctga cttcaatttt ggccgcatac tcgaccggaa
      121 agcctgctta tgactcaaga ctgaaagcat acaccaattt ctgcaaagac aaggctattc
      181 cggcaggaac tgagtcactg caaaaatata tcaaatccct gcattccgtt tccacaaaga
      241 accttacaaa aactgcagtc atgacagcat taaaatctat atacgaaaat gaacctgcaa
      301 agctgtatga gctggagaaa gttttcaaga gcgtcaaggc tgggagaaaa aacagcattg
      361 ccataaaagc agatgaggtt tactcggagg aaactgtgaa tgtcatgatt gaagaacttt
      421 ccagcagaca gaacctctca aagaaaaata atactgatcc tgccaagttt cagaaactcg
      481 gtctcatcgt ctggactctc tttaataccg gtctgagaat ttcggagttg acagggatca
      541 ggaatcagga tgtgcacctg aacgggaaag cgacaatcaa agttctaggg aaaggatcta
      601 aggaaaggac agtgtatctg ccgaaagacg ctgtgatgga aatcagggag gttttcgagt
      661 ctgctgattc tgagtattta ttttgcaata tgaggcaagg cagattgaac agagagaact
      721 tgacaaatga attgagcaag gcattgaaaa aagattatcc agattttcac ccgcacaaac
      781 tcagacactc ctttgctaca aaggaaatta tcagcaagaa gaagagcgtc aaggcagtca
      841 gtaaatacct ggggcactcc agcactgcca ttacgcagga tttatatgtg catgatgaga
      901 tcgtatttga tgacttggaa tttacagaaa ggaaaagagc atgaacacac tagaacagaa
      961 aattgataac ctcatagaaa gattgcagag gactccagac ataggaatga tccaagcagg
     1021 aaaggaattc aggattgtca accggtcaac cggcgaagtg ctggattcat gctatgatga
     1081 gggatatgc
//
LOCUS       DAOQGJ010001837         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116534_length_1089_cov_3.881044, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001837 DAOQGJ010000000
VERSION     DAOQGJ010001837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116534_length_1089_cov_3.881044"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..>1089)
                     /locus_tag="PK683_21910"
     CDS             complement(158..>1089)
                     /locus_tag="PK683_21910"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH11169.1"
                     /translation="GITETKDIRMEKLEEDIKTSGICFFFLEIPSSLSMDSLAPFDYA
                     SHSVLFKSMKCFSMKRTNFIESAADILGKFINFPIYEISPKEMRLPYGTTGDKYLKKD
                     GSNIVSFLDYLKDNQEDIYAGLKESLSLITKDFQGFRLEALRKGYNEIKSKFSSDTIK
                     RFGLIDRKNKDNTIWGEYLSDGILYFLALLCVVSQPYSPPVLLLEEPEKGIHPRRIQE
                     VMDLLFVLAEEKMVQIILTTHSTFVLDQFSDIPENISVFENHDSVITVKNLKKDVLDK
                     IDADFKEQGYANEDFTEALGENWALGLLGGIPD"
BASE COUNT          335 a          219 c          176 g          359 t
ORIGIN      
        1 gatatcactt ttttgaaact tctgggagcc tttacatcat tttcaaaatt tcgatcagga
       61 taaaattgga tttgttcatt acttatctgt ttcaaaaaat ttcggatgaa tattctgtga
      121 ggatcatcct ctccaaaaac tccatacttg acttgtttca atcaggaatt cctccaagca
      181 gtcccaaggc ccagttttca cccaatgcct cagtgaaatc ttcgttagca tatccctgct
      241 ctttaaaatc tgcgtcaatc ttgtccagaa catctttttt taaatttttc actgtaatga
      301 ctgaatcatg attttcgaat acactgatgt tttctggaat atcactgaac tgatccagaa
      361 cgaatgtaga gtgcgtagtt aaaattatct gtaccatttt ttcctccgcg aggacaaaaa
      421 gcagatccat aacttcctga attcgcctag ggtgaattcc tttttctggt tcttcgagaa
      481 gaagcacagg cggtgaatag ggctgagaca caacacagag aagtgctaaa aaatatagaa
      541 taccgtcaga aaggtattct ccccagattg tattgtcctt attttttctg tcaatcagcc
      601 cgaacctttt aattgtatca gaagagaatt tactttttat ttcattatac cctttacgca
      661 atgcttccag acgaaatcct tgaaaatcct ttgtaatgag agacagagat tcctttaatc
      721 cagcatatat atcttcctga ttatctttca gataatccag aaaagataca atatttgaac
      781 cgtctttttt aagatatttg tcccctgttg ttccataagg aagacgcatt tccttagggg
      841 aaatttcata tatagggaaa tttataaatt ttccaagaat atcggcggca gactcaataa
      901 aattggttcg tttcattgaa aaacatttca tacttttaaa aaggacagaa tggctggcat
      961 aatcaaaagg agcaaggctg tccattgaga gactggaagg aatctccaag aaaaaaaaac
     1021 aaattccact tgtcttgata tcttcttcca gcttctccat ccgaatatct tttgtttcag
     1081 ttatccccc
//
LOCUS       DAOQGJ010001838         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116573_length_1089_cov_3.135397, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001838 DAOQGJ010000000
VERSION     DAOQGJ010001838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116573_length_1089_cov_3.135397"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PK683_21915"
     CDS             complement(<1..1062)
                     /locus_tag="PK683_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH11170.1"
                     /translation="MSSTEEGILVLKSVRNDFGISDFECAYTNKSAEEIIQKSNEYLL
                     GKRILQELPFIGETGLFEKCVNVAETGVHVRNDHLIQHEGKNAWLHFSCVKYNDGVIL
                     VFSDITKEKQNELIMLQSAERLRLVTENAPDVILELDREGTVLFISRTAAGLKKEDIL
                     GKNYLHWVPEESRSRLTDLLNQAFETGHLQSYEVYAQGGEGAMSWYSSVLSPVVLNGK
                     TQSVILSARDITERKNQEILLIQAREQAEAGNRAKSEFLASMSHEIRTPLNGIIGFTD
                     LLMQTKLNEAQRNYMEIVSKSANSLLDLLTDILDFSKIEAGKLELNIEKLDIHELAGQ
                     ASDIVKYRAREKNLELLLNI"
BASE COUNT          257 a          276 c          205 g          351 t
ORIGIN      
        1 gatattcagc agaagttcca gatttttttc ccttgccctg tattttacaa tatctgatgc
       61 ctgtcctgcc agttcgtgaa tgtcgagttt ctcaatgttc agttccagtt ttcctgcttc
      121 aattttagaa aaatccaata tgtctgtcag cagatcaagc agagaatttg ctgacttgct
      181 tacaatttcc atataattcc gctgtgcttc attcagtttt gtctgcatca gaagatcggt
      241 gaaaccgatg attccgttca agggtgttct tatttcgtgg ctcatgcttg caaggaattc
      301 agactttgcc cggtttcccg cttccgcctg ttccctcgcc tgaatcagaa gtatttcctg
      361 gtttttcctt tccgtaatgt ctcttgcgga aagaatgaca ctctgagtct ttccgttcag
      421 aacaacagga gacaggactg aagaatacca gctcatcgca ccttctccgc cctgtgcata
      481 gacctcatag gactgcagat ggcctgtttc gaatgcctga ttgaggagat ccgtcagacg
      541 actcctcgat tcttcaggca cccagtgcag atagtttttt cccagtatgt cctctttttt
      601 cagtccggca gcggtacggc tgatgaaaag aaccgtcccc tctctgtcga gttccagaat
      661 tacatccggg gcattttccg ttacaagccg aagacgttct gcagactgaa gcataatgag
      721 ttcattctgc ttttccttcg taatgtccga aaaaaccagt atgactccat cattatattt
      781 tacacaggaa aaatgcagcc atgcattttt cccttcgtgc tgaatcagat gatcatttct
      841 gacgtgaact cctgtttccg ccacattcac gcatttctca aataaccctg tttccccgat
      901 aaaaggaagc tcctgcagaa ttctttttcc tagcaggtat tcattgctct tctggataat
      961 ttcttctgca gacttgttgg tgtaagcaca ttcaaaatca gaaattccaa aatcattcct
     1021 gactgatttc agaaccagta ttccctcttc cgtgctgctc attatcccct gcaggaaact
     1081 gtctttttc
//
LOCUS       DAOQGJ010001839         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116869_length_1088_cov_1.972894, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001839 DAOQGJ010000000
VERSION     DAOQGJ010001839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116869_length_1088_cov_1.972894"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>1088
                     /locus_tag="PK683_21920"
     CDS             2..>1088
                     /locus_tag="PK683_21920"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNH11171.1"
                     /translation="MLSDEEIENFGKELEVIRTEIQSKVGQEDADYIRNVYNVMRYNE
                     IMGRLLIHFSVDPVTFAIGTGMLGISKILNNMEVGHNVLHGQYDFMNDPELNSRTFDW
                     DHSCDSDQWKFYHNYMHHTYTNIVGKDYDFGYGFIRLSEDQKWKPLHLLQSVANVFLC
                     LGFEWGVGSHGPNVKFLETPRKQRNRNTKKELNDVYYKKMERQLFKDYVLFPLLGGIM
                     APKIMLGNFMANIMRNVWTYAIIFCGHFTEHTETFPVETLENETKAEWYLRQLKGSSN
                     LEGSRLFYILSGHLSHQIEHHMFPELPANRYEEIAPRLKALCEKYGQYYNNGSFLSQF
                     GSVVKRIIAYSFPDETARKFTKKSPKGA"
BASE COUNT          341 a          207 c          250 g          290 t
ORIGIN      
        1 agtgctgtca gacgaagaga tcgagaattt cggaaaagaa ctggaagtca tcaggacaga
       61 gattcagtcc aaggtgggac aggaagacgc agattatata cggaatgtat ataatgtaat
      121 gagatataat gaaattatgg gaagacttct gattcatttc agtgtggatc ctgtgacttt
      181 tgcaataggg acaggaatgc tcggaatttc caagattctg aacaatatgg aagtagggca
      241 taatgtgctt cacggtcagt atgactttat gaatgatccc gaactcaatt ccaggacatt
      301 cgactgggat cattcctgtg attcggatca gtggaaattc tatcataatt atatgcacca
      361 cacatataca aatattgtag gcaaagacta tgatttcgga tacggattca tccgcctttc
      421 ggaagatcag aaatggaagc cgcttcatct tcttcagtca gtggcaaacg tattcctctg
      481 tcttggattt gaatggggtg tcggttcaca cggtcccaac gtcaaattcc tggaaactcc
      541 cagaaagcag agaaacagga acacaaagaa agagctgaat gatgtgtatt acaaaaaaat
      601 ggaaagacag cttttcaaag actatgttct ttttcctctt ttggggggaa tcatggctcc
      661 caagattatg ctggggaatt ttatggcaaa cattatgaga aacgtatgga cttatgcgat
      721 catcttctgc ggtcatttca ctgagcatac tgagactttc cctgtggaaa cactcgaaaa
      781 tgaaaccaag gcagagtggt atctgcgtca gctgaaaggt tcctcaaacc tcgaaggaag
      841 cagactgttt tatatactct ccggtcatct gagtcatcag attgaacatc atatgtttcc
      901 tgagcttcct gcaaacagat atgaggagat tgctccgaga ctgaaggctc tctgcgagaa
      961 atatggtcag tactacaata acggaagttt cctgagccag ttcggttcag tagtgaaaag
     1021 aatcatcgcc tattcatttc ccgatgagac tgccagaaag tttacaaaaa aatcacctaa
     1081 gggagcag
//
LOCUS       DAOQGJ010001840         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116889_length_1088_cov_1.802517, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001840 DAOQGJ010000000
VERSION     DAOQGJ010001840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116889_length_1088_cov_1.802517"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1088)
                     /locus_tag="PK683_21925"
     CDS             complement(<1..>1088)
                     /locus_tag="PK683_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11172.1"
                     /translation="PLSFQLLLDTERFRKIPLSIQIFSLLKVLEIHLNPSSDLKIAAE
                     DNDNVLQASLKSFLTVKNDSTKSPKELFSSLNFGTENAVLTVKKQKPQNINFKILYEL
                     GIIPEKDSLELKEFTAVFAGSKWLDIRGSVSGLTKEKPYIDMKAAQSKIVLDPLGKLL
                     SSLPGVRNLGISGQLNIDAVSAKGDIDTMLAKADIRGSNLLINSGGSSHSIPTLRLDA
                     ETVLNLSDKAKSTEKDLIPVLEKADLKTVELVYNGISAKLKGTVIPKSKVDLTLKVDN
                     VNLAQFSPEISGIVRADAAASGNSLSLVDINLKANADGVRYRMGKSISGKNFLYASLK
                     TTLDFGGGFKFEDLQIHPADIILKNENG"
BASE COUNT          303 a          239 c          241 g          305 t
ORIGIN      
        1 cccgttttcg tttttcagaa taatgtctgc aggatggatc tgcaaatctt caaacttgaa
       61 tccgcctccg aaatccagag tggttttcag ggaagcataa aggaagttct ttcctgaaat
      121 ggattttccc atcctgtagc gcacaccgtc tgcatttgcc ttgagattga tatcaaccag
      181 agaaagcgaa tttccggaag ccgctgcatc ggccctcaca atacctgaaa tttccggaga
      241 aaactgagcc agattgacgt tgtctacctt aagagtcaga tcaactttgg acttaggaat
      301 gacagtgcct ttcagctttg cagaaatccc attgtagaca agctcaacgg tcttaagatc
      361 agctttttcc agaacgggta tcagatcttt ttctgtggac tttgccttgt ctgaaaggtt
      421 gaggacagtc tctgcatcaa gccgaagagt cggaatggaa tgagaagaac cgcctgaatt
      481 gatcagaaga ttagaaccgc gaatatctgc ctttgcaagc attgtatcaa tgtcgccttt
      541 ggcagaaact gcatcaatgt tcagctgacc ggaaatcccc agatttctga ctcccggaag
      601 cgaagaaaga agttttccca gaggatcgag aacaatcttc gactgagcag ccttcatatc
      661 aatgtagggt ttctcttttg taagtccgct tactgacccg cgtatgtcca gccatttcga
      721 gcctgcaaaa acggcagtaa attccttcag ttccagagaa tccttttccg ggatgattcc
      781 cagttcatac aggattttaa aatttatgtt ctgcggtttc tgcttcttca cagtcagtac
      841 cgcgttttcc gttccaaagt tcagagagga aaacagttcc ttcggacttt tggtggaatc
      901 gttttttacc gtaagaaaag acttcagcga agcctgcaat acattatcat tatcttctgc
      961 tgcaattttc agatcagagg aaggattcag atgaatctcc agaactttca gcagagaaaa
     1021 gatctgtatg gaaagaggga ttttacgaaa tctttctgta tccaggagga gctggaagga
     1081 aagaggac
//
LOCUS       DAOQGJ010001841         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116977_length_1087_cov_3.071705, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001841 DAOQGJ010000000
VERSION     DAOQGJ010001841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116977_length_1087_cov_3.071705"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1087)
                     /locus_tag="PK683_21930"
     CDS             complement(<1..>1087)
                     /locus_tag="PK683_21930"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNH11173.1"
                     /translation="KTVKTGDLVKGIAEFPSLEELPAIKSWPLDGGRFITLPLVYTES
                     PVTGKGNLGMYRVQIHDKTTAGMHIQIHRGGGFHYYEAEQKNISLPVHIYAGGPPALT
                     LSAIAPLPEDISELLLASLILGEKVKVIRNSQISPLPIIADADFCIIGEIPPHKRKPE
                     GPFGDHYGYYALQHDYPYADIKRILYRKDAIWPLTVVGRPPQEDHYIAEYLQELLSPL
                     FPLVMPKVVNVWAYEESGVHSLTAAVVKERYHKEAFTAALRILGEGHLSLAKCLFVTN
                     QMVNLRNFKETLVTVLERVNVRTDVHIFSNISQDTLDYTGPAVNSGSKMVVLGIGEKR
                     NELAKDFFSDFKNSIFKNPKVFVPGALV"
BASE COUNT          288 a          248 c          235 g          316 t
ORIGIN      
        1 ctacaagagc tcccggaaca aatactttcg gattcttgaa aatcgaattc ttaaaatcac
       61 tgaaaaaatc ttttgcaagt tcatttcttt tttcaccgat cccgaggacc accatcttgc
      121 tccctgagtt gactgcaggg ccggtatagt ccagtgtatc ctgtgatata tttgaaaaaa
      181 tatgcacgtc tgtcctgaca tttactcttt cgagaacagt cacaagagtc tccttaaagt
      241 tccggagatt taccatctgg tttgtcacga aaagacattt tgcaagagaa agatgacctt
      301 ctccgagaat cctcaaggcg gcagtaaaag cttccttgtg atatctttcc ttcactactg
      361 cagcagtcag actgtgaact cctgactctt catatgccca tacattcact actttcggca
      421 ttacaagggg gaatagaggt gaaagcagtt cctgaagata ttcagctatg taatgatctt
      481 cctgaggcgg ccttcctaca accgtaagcg gccatattgc atcttttcta tataatattc
      541 tctttatatc agcataagga taatcgtgct gcagggcata gtatccgtaa tgatctccga
      601 aaggcccttc cggttttctt ttatggggag ggatttcacc gataatgcag aagtctgcat
      661 cagcgatgat cggaagggga gaaatttgac tgtttctgat tacctttacc ttttcgccga
      721 gaatgagaga tgcaaggagc agttctgaaa tatcctcagg cagaggagcg attgccgaaa
      781 gagtaagagc aggagggcct cctgcataga tatgaacagg aagtgagata tttttctgtt
      841 cggcctcgta ataatgaaac ccgcctcccc ggtggatctg aatatgcatt cctgcagtgg
      901 ttttatcatg gatctgcact ctgtacattc ccagattgcc ctttcccgtg acaggacttt
      961 cggtataaac cagcggaagt gtaatgaatc tccccccgtc aagcggccag gattttattg
     1021 caggcagttc ttccagagaa ggaaattctg caattccctt gaccaggtcg cctgttttta
     1081 ctgtctt
//
LOCUS       DAOQGJ010001842         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_116996_length_1087_cov_2.851744, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001842 DAOQGJ010000000
VERSION     DAOQGJ010001842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116996_length_1087_cov_2.851744"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK683_21935"
     CDS             <1..214
                     /locus_tag="PK683_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11174.1"
                     /translation="TDRLVKAVFGASTNVKKTVISCLNSFSASLFLEEMENYSHTASR
                     NWDSEEREIISAQEYLMQMIRKIDGH"
     gene            complement(208..>1087)
                     /locus_tag="PK683_21940"
     CDS             complement(208..>1087)
                     /locus_tag="PK683_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11175.1"
                     /translation="ISSLNRLNAEWVRLEFNYYDPLPDSLMSDFVSMLDESEIKILGL
                     LTGLVPGNLINCLIPSVRFKCPLDTLDHYKEFCLKQAELFGDRIKHWEIWNESNTLRF
                     WIRKPNSAEYTELVKETVGAMRPNVSEGTKFVFGGLMGDDVHVYAPFQKINFIQECAD
                     LGIDDYIDIYSFHPYIPACYVSRKPKEHYITGIKESAETYLKKYSHLKKPQWISEIGI
                     CPLWTSVGQEKIGEIYAELYRHYNALDIPMFLWTLTDFKGKEYSRFNPETAFGLLDWD
                     LNEKPVFESLLKNLNQ"
BASE COUNT          310 a          254 c          234 g          289 t
ORIGIN      
        1 cacagacagg ctggtaaagg ccgtgttcgg ggcaagcacg aatgtaaaaa agactgtcat
       61 ttcgtgcctg aattcattct cagcctcgct gtttctggaa gagatggaaa actattccca
      121 tactgcatcc aggaattggg attcggaaga aagggaaatc atcagcgcac aggaatacct
      181 gatgcagatg atccggaaaa tcgacggtca ctgattcaga tttttcaaaa gactctcaaa
      241 aacaggtttt tcattcagat cccagtccag aagtccgaat gcagtctccg gattgaacct
      301 ggaatattcc tttcccttga agtctgtcag agtccataga aacatcggga tatccagtgc
      361 attgtaatgc ctgtaaagct ctgcataaat ctcccctatt ttttcctgac cgacggaggt
      421 ccagagagga cagattccta tctcgctgat ccactggggc tttttcagat gactgtattt
      481 tttcagatag gtttctgctg attcctttat acctgtgata taatgctcct tcggcttcct
      541 ggaaacataa caggcaggga tataaggatg aaaactgtaa atatctatat aatcatcgat
      601 gcccagatcc gcgcattcct ggatgaaatt gattttctga aaaggggcat acacatggac
      661 atcgtccccc atcagccctc cgaaaacaaa cttcgttccc tctgagacgt tgggacgcat
      721 tgcgccgaca gtctccttca ccagctcagt atattccgca gaattcggtt ttctgatcca
      781 gaaacgcagt gtatttgact cattccagat ttcccagtgc tttatccgat ctccgaacag
      841 ctccgcctgc tttaagcaga attccttata atgatcaagt gtatccagag gacatttgaa
      901 acgaacagaa ggaataagac agttgatcag atttccggga accaggcctg tcaggaggcc
      961 gagaatctta atttcggatt catcaagcat cgacacaaaa tcggacatca gtgaatcagg
     1021 aagcgggtca taataattga attccagtct gacccattct gcattgagcc tgttcagcga
     1081 ggaaata
//
LOCUS       DAOQGJ010001843         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_117403_length_1085_cov_4.062136, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001843 DAOQGJ010000000
VERSION     DAOQGJ010001843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117403_length_1085_cov_4.062136"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..411
                     /locus_tag="PK683_21945"
     CDS             112..411
                     /locus_tag="PK683_21945"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNH11176.1"
                     /translation="MSSLTKVELKIFGQDYIIAGEASEEYIRSLGLFVNSKMEELSKV
                     FPNANPVKLAVLAAVNIADELFQIKEIPMNAEVVSMYEEKTKMLISMLDKGLIGE"
     gene            419..>1085
                     /locus_tag="PK683_21950"
     CDS             419..>1085
                     /locus_tag="PK683_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11177.1"
                     /translation="MEKKTETALKKEKIYSFSNYLSLLSRIYLKEIQKQSSSKPTGVV
                     HRKAVSRETAFTVNRVRGKVKEFPVHNGRPRFENSRIETPESRQRLSSDLISLYWEIS
                     FLNSRKSILDEVFQKARTMKKLKLALDAVEEICISRTETDRYARYNETLSLYKEKKKQ
                     IAAVLLKDRTISEFEIDYILFDRISDQHRFEDSAWNDFRERYFSQEGDYCRESAESFH
                     RKIM"
BASE COUNT          368 a          187 c          233 g          297 t
ORIGIN      
        1 gatatcatta ttgatgaaac tgatgatgct atcaatgtat ttgattctgc tgaagatgat
       61 gatttcctga ttattgaaga ggaaattaag tagtcttagg gatttttgga tatgtcctcc
      121 ctgactaaag ttgaactcaa aatctttggt caggactata taattgcagg agaggcctct
      181 gaagaataca tccgttctct aggtcttttc gtaaattcga aaatggaaga actcagcaaa
      241 gtttttccaa atgcaaatcc cgttaaactc gcagttctgg ccgcagtgaa cattgcggat
      301 gaactctttc aaataaagga gattcctatg aatgctgagg ttgtctctat gtatgaagaa
      361 aaaactaaaa tgctgatttc tatgctggat aagggactca taggagaata actctcttat
      421 ggaaaagaaa accgaaaccg ctctcaaaaa agaaaagatc tattcttttt ccaattatct
      481 ttcccttctc agcagaatat atctgaaaga aattcaaaaa cagagcagca gtaagccgac
      541 aggtgttgtt cacaggaagg cagtctcccg tgaaactgca tttacagtaa atcgggttcg
      601 gggcaaagta aaagagttcc ctgtccataa cggcagaccc agatttgaaa acagcagaat
      661 tgaaactcct gagtcccggc agagactgag ttctgatctg atttcactgt actgggaaat
      721 cagttttctg aattccagga aaagcatact tgatgaagtg tttcagaaag ctcgtacaat
      781 gaaaaaactg aaacttgcac tggatgcagt ggaagagata tgcatcagca gaacagaaac
      841 ggacagatat gcaagatata atgaaacttt atctctttat aaggaaaaga aaaagcagat
      901 agcggcagtt cttctgaaag acagaacaat ttccgaattt gaaattgatt acattctttt
      961 tgacaggatc tccgatcagc acagatttga ggattctgca tggaacgatt ttcgagagag
     1021 gtatttcagt caggaaggtg attactgcag agaaagtgcc gaaagttttc accggaaaat
     1081 aatgg
//
LOCUS       DAOQGJ010001844         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_117443_length_1085_cov_2.989320, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001844 DAOQGJ010000000
VERSION     DAOQGJ010001844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117443_length_1085_cov_2.989320"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1085
                     /locus_tag="PK683_21955"
     CDS             <1..>1085
                     /locus_tag="PK683_21955"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNH11178.1"
                     /translation="SASFPYYFFSDNCSYHLLSLLEVARPSLRLREKLPGWVIPSETI
                     KVISEEKDLVAEKLYRPSLHSRIRQKLFLMSREEREILYKVLGREISPDKFREMNIPD
                     DRKAFLLDALLDSFRFRKANKNQENAEDALYRKFLSVRTELPAEKDTVYSPVTTSPDT
                     GHSIIRIKTGIGYSTKGSFAEFSLRPAHHDLLNQDSGYFPNSENLVLNFRLRAYNEDK
                     RLFLEETNILKLASFTPYNSISKLNSYFVNFGSDAAVIKKDRLLRDPDYRRQSMVNTE
                     ALYGFTFQDEYSPSFSQFSLSILAGMKVQSSPLLERGYRAGPQVLFNLIGDFGNIKFL
                     LSTAYYGFSFLTGQNDYKASFQVRYSL"
BASE COUNT          325 a          235 c          216 g          309 t
ORIGIN      
        1 gaagtgcttc attcccttat tacttctttt ctgacaactg ctcctatcat cttctttcac
       61 ttcttgaagt tgcaaggcct tctttgagac tgagagaaaa acttccagga tgggtcattc
      121 cttctgaaac aataaaggtg atatctgaag aaaaagatct ggtagcagaa aaactgtacc
      181 gcccctctct gcacagcaga ataagacaga aactctttct gatgagccgg gaagaacgtg
      241 aaattctgta taaagtgctg ggaagagaaa tcagtcctga caagttcaga gaaatgaaca
      301 ttcccgatga caggaaagcc ttccttctcg atgcgcttct tgattcattc agattcagaa
      361 aggcaaataa aaatcaggaa aatgccgagg atgcattgta caggaaattt ctttcagtga
      421 gaactgaact tccggcagaa aaagacactg tttacagtcc tgtcacaacg tctcctgata
      481 caggccattc cattatcaga ataaaaaccg gaatcggata ttcaactaag ggctcttttg
      541 cggaattttc gcttcgcccc gctcatcatg acctgctgaa tcaggattcc ggatattttc
      601 ccaattcaga aaaccttgtt ttaaatttca gattgagagc atacaatgaa gataagagac
      661 tgttcctgga agaaacaaat atactgaaac tggcttcatt cactccttat aattccattt
      721 ccaaattaaa ttcgtacttt gtaaatttcg gctcggatgc tgccgtaata aaaaaggacc
      781 gtctgctccg cgacccggat tacagaagac agagtatggt aaacacggaa gcattgtacg
      841 gtttcacttt tcaggatgaa tacagcccaa gtttttcaca attttcactt tcaattctag
      901 ccggaatgaa agtgcagtcg tctcctcttc tggaaagggg atacagagca ggcccgcagg
      961 tgcttttcaa tctgatcgga gatttcggga acattaaatt tcttctgtca actgcgtatt
     1021 atggattttc cttcctgacc ggacagaatg actataaggc atcttttcag gtccgttatt
     1081 ctctg
//
LOCUS       DAOQGJ010001845         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_117493_length_1085_cov_2.402913, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001845 DAOQGJ010000000
VERSION     DAOQGJ010001845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117493_length_1085_cov_2.402913"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PK683_21960"
     CDS             complement(<1..906)
                     /locus_tag="PK683_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH11179.1"
                     /translation="MHLPEFLLSYHSIGVFLVSVILLIETLFLVFKRDKKKPSYWLIG
                     VFTGFTVMLVGYFLAYSVYDPIGAFHRYLTVFVLFGNFCFVGFAYTFLKEDYQKEYRL
                     IMPFLFLTALGAYIHFIWNTYSAEKNYNFEAHCYNFNFGTETSLFILFFFLWSLFILV
                     RKTVSYSLYDGFFKKWLIVPGEPSFLFRIQNALARFCAGFICLFFPRGKAANSTKTFI
                     LIIMFLIITAVSNVLNKAGILPYDYYAVYYSNSTLVICFLMLLGYMNSSGESTTFMVK
                     LVGISLLAVLLVLGFVSNLTLNISES"
BASE COUNT          370 a          219 c          235 g          261 t
ORIGIN      
        1 ggattcgctg atgttcaggg tcagattgct cacaaaaccc agaacaagca gaacagccag
       61 aagactgatc cctacaagct ttaccatgaa tgttgtgctt tcccccgagc tgttcatgta
      121 gcccagaagc atcagaaagc agatcaccag agtggaattt gaatagtaca ctgcgtaata
      181 gtcgtaaggg agaattcctg ccttgttcag tacattggaa acagcagtga taataagaaa
      241 cattataatc agaatgaatg tcttagtgga atttgcagcc tttcccctgg gaaagaaaag
      301 acagatgaat cctgcacaga atcgggcaag tgcattctga atgcggaaaa ggaaggaagg
      361 ctcgcccgga acaatcagcc attttttaaa aaaaccgtcg tacagagagt agctgactgt
      421 ctttcggacc aggatgaaga gtgaccagag aaagaaaaat aaaatgaaga gtgatgtttc
      481 cgttccgaaa ttgaagttgt agcagtgagc ttcaaaattg taattttttt ctgcggaata
      541 ggtgttccag atgaaatgga tgtatgcacc caaggctgtc agaaaaagaa aaggcataat
      601 cagacggtat tccttttgat aatcttcctt gaggaaagtg taggcaaaac cgacaaagca
      661 gaaattcccg aaaagtacaa atactgtcag atatctgtga aaggctccga ttggatcata
      721 cacggagtat gccaggaaat agccgacaag cattacagta aagccggtaa acactccgat
      781 gagccagtag cttggctttt tcttgtctct cttgaataca agaaacagag tttctatcag
      841 cagaatcacg gaaacaagga atacaccgat agaatggtaa gagagaagaa actccggtaa
      901 gtgcatggcc gaaaacctaa tttttaaccc gaataattac aagcaaaaaa ctaaagataa
      961 tcatctttat ccagttccac tgcacccctg tgcggatatg cctgctcaaa aaaaagtgcc
     1021 gcaaaaagtt caaattcctc atcctccgga agatcattct cttcccagtg catatttctt
     1081 ctttt
//
LOCUS       DAOQGJ010001846         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118068_length_1082_cov_2.967868, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001846 DAOQGJ010000000
VERSION     DAOQGJ010001846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118068_length_1082_cov_2.967868"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1082)
                     /locus_tag="PK683_21965"
     CDS             complement(<1..>1082)
                     /locus_tag="PK683_21965"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNH11180.1"
                     /translation="YNYADTAEAFVSQFEVPKASLEPGRYRNITGNAAIALGFVVASK
                     KCHLQLFLGSYPITPATDILHELSKYKNYGVKTFQAEDEIAGICSALGASFGGSLGIT
                     TTSGPGVCLKTEAMNLAVMTELPLVIVNVQRGGPSTGLPTKTEQSDLLQALFSRNGES
                     PIPVIAASTPASCFAMTMEAVRIALEFMTPVILLSDGSIANGSEPWKLPLLESLPEIR
                     HRKVTENNNPKGGFLGFKRDSRLVRDWAVPGVPGLEHRIGGLEKDYNTSSVSSNPENH
                     EKMVLTRIEKIANISNYIPLQDVYGDESGGTLLVGWGSTFGSIRSAVEELREEGVKVS
                     HAHIHYLNPFPKNLGELMSRYDKIIV"
BASE COUNT          285 a          274 c          216 g          307 t
ORIGIN      
        1 aacaatgatt ttatcatacc tggacatcag ttctcccaga ttcttaggaa aaggattcag
       61 ataatgaata tgagcatgag aaacttttac tccctcttct ctcagttctt ccacagcact
      121 tctgatagaa ccgaaggtgg aaccccagcc tacaagcaga gttccgcctg actcgtctcc
      181 ataaacatcc tgtaatggaa tataattaga aatatttgca attttctcaa ttctggtaag
      241 caccattttt tcatgattct caggatttga gcttacgctg cttgtattgt aatctttttc
      301 cagtcctccg attctgtgct caaggccggg cactcccgga acagcccagt ctctgacaag
      361 cctgctgtct cttttgaatc ccagaaaacc gccctttgga ttattattct ctgtcacttt
      421 cctgtgtctg atttccggca gggattccag cagtggaagc ttccaaggct cagaaccgtt
      481 tgcaatagaa ccgtcagaaa gaagtatgac aggagtcata aattccaatg cgattctgac
      541 ggcttccata gtcatcgcaa aacagctggc cggagtgctc gctgcaatga ccggaatagg
      601 cgattcaccg tttctcgaaa acagtgcctg aagcagatca gactgctccg ttttagtcgg
      661 tagacctgtc gaagggccgc ctctctgcac attcacaatt acaagcggga gctcagtcat
      721 cacagccaga ttcattgctt cagtcttcag acatacacca gggcccgacg tagttgtaat
      781 tcccagactt cctccgaaag atgcgcccaa tgcactgcag atccctgcta tttcatcttc
      841 agcctgaaat gtctttactc cataattttt atacttggaa agttcatgca gaatatcggt
      901 cgccggtgta atcggataac ttcctaagaa taactgcaga tgacattttt tagatgcaac
      961 tacaaatccg agagcgatcg ctgcattgcc tgtgatattc cgataccgtc caggttccag
     1021 tgaagctttc ggcacttcaa actgagatac aaatgcttct gcggtatctg catagttata
     1081 tc
//
LOCUS       DAOQGJ010001847         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118160_length_1082_cov_1.808179, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001847 DAOQGJ010000000
VERSION     DAOQGJ010001847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118160_length_1082_cov_1.808179"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK683_21970"
     CDS             <1..293
                     /locus_tag="PK683_21970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11181.1"
                     /translation="TVSLVKTRDTQQSIKIGGYTFSLNFNFLLGNGILQLGLIKQEAQ
                     LTSMMNDYLMYSFGPKGPSVDWGSFLIKQMLNINEKDVFQGITLGYQHKLSD"
     gene            303..>1082
                     /locus_tag="PK683_21975"
     CDS             303..>1082
                     /locus_tag="PK683_21975"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11182.1"
                     /translation="MKKICIIIITLFLLIHCGESFRPIRNVPEKAKKVVLFLDFENNS
                     IGSGKDNWDGLRRAIPSMILTDFKNFGTYRPVTEDARLSALKELKRVQQGILEDRGIQ
                     AGKLASADFILSGNFMEISSSIAITAKLTDVQTGEAVSSSSQQGHVDSILNPPEKSLI
                     KKTSITLLSQTQYNPTTNEIQMIADVIETKKIQAAGKNYEGEIIMDDVALLKKSGKSA
                     ESQEVKSLEEKANWKFKSAVSEDPSYERAKQNMEATVSLLPP"
BASE COUNT          367 a          217 c          218 g          280 t
ORIGIN      
        1 atacagtatc tttagttaaa acaagagata ctcaacaatc tataaaaatt ggtggttaca
       61 cattttcact gaattttaac tttctgttag gaaatggcat tcttcaactc ggtctaatta
      121 aacaggaagc tcaattgact tcaatgatga atgattatct tatgtattcc ttcggtccaa
      181 agggaccaag tgttgattgg ggaagttttc taatcaaaca aatgctgaat atcaatgaaa
      241 aagatgtttt tcaaggaatc actttaggct accagcataa actgtctgat taaggagtat
      301 atatgaaaaa aatatgcatc ataattatta cactgtttct gctcattcac tgtggagagt
      361 cattcagacc gattcgaaat gtgcctgaaa aagccaaaaa agtggttctg tttctggact
      421 tcgaaaacaa cagcatcggt tccggaaagg acaattggga tggactcaga agagcaattc
      481 cttccatgat tctgacagat ttcaaaaatt tcgggactta cagaccagtc actgaggatg
      541 ccagactttc tgctttaaag gaactgaaaa gggttcagca gggaattctc gaagacagag
      601 gcattcaggc aggaaaactc gcgagtgctg actttattct ctccggaaat tttatggaga
      661 tttccagttc gattgccatc actgccaaac tgactgacgt tcagacaggc gaagctgttt
      721 cttcctccag tcagcagggg cacgtagaca gcattctgaa tccgccggaa aaatcactca
      781 tcaaaaaaac atccatcact cttctttcgc agacacagta caatcctaca acaaatgaaa
      841 tccagatgat tgcagatgtc attgaaacaa agaaaattca ggctgctgga aagaattacg
      901 agggagaaat cattatggat gatgttgcac tcctgaagaa atcaggaaaa tcagcagaga
      961 gccaggaagt gaaatctcta gaagaaaaag ccaactggaa attcaaatca gcagtctcgg
     1021 aagacccttc ctacgaacgt gcaaaacaga atatggaagc cacagtcagt ctcctcccgc
     1081 ct
//
LOCUS       DAOQGJ010001848         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118251_length_1081_cov_2.872320, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001848 DAOQGJ010000000
VERSION     DAOQGJ010001848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118251_length_1081_cov_2.872320"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1081
                     /locus_tag="PK683_21980"
     CDS             <1..>1081
                     /locus_tag="PK683_21980"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH11183.1"
                     /translation="GIKPIEPREEGLPVVFKVVPGILREICNRIRSIERLKGNETEIH
                     QKVDNFQDLYNAFLKRLNDILSELEENEPYFIEARRSKVKILEIEGSNIITIGEKAST
                     KETVMRDKIKRIYDKFDSPDDITDVVNQLREVGTDIGWTTNYFAVFKALKEFETSMVN
                     VQKKESSKSYKQNYVLIIDEINRGNVSQIFGELITLIEEDKRIGGKEELEVTLPYSKE
                     SFGVPSNLYIIGTMNTADRSVEALDTALRRRFHFEEMMPDPKLIADEGLVQNGILEGI
                     DLVKLLNTINERIVLLLDKDHQIGHSYFMAVENLEDLKSVFKNKIIPLLQEYFYGDYG
                     KIRLVLGDKFIEKADGKNITFAVKDE"
BASE COUNT          385 a          153 c          246 g          297 t
ORIGIN      
        1 ggaataaaac ctatagaacc gagggaagaa ggacttcctg tagtatttaa agtggttcct
       61 gggattttgc gggagatctg caacagaata agaagcatag aaagactgaa aggaaatgaa
      121 acagagattc atcagaaagt agacaatttt caagatcttt acaatgcatt tttgaaacgt
      181 ctaaacgata tactctctga attagaagag aatgaaccct attttattga agctagacgt
      241 tcaaaagtaa aaattcttga aattgaggga agtaatatca ttacaattgg tgaaaaagca
      301 agcactaaag aaacggtaat gagagataaa ataaaaagaa tatatgataa atttgacagt
      361 ccggatgaca ttactgatgt agtgaatcaa ttacgcgaag tagggactga tattgggtgg
      421 actacaaact attttgctgt attcaaagct ttaaaggaat ttgaaacttc tatggtgaat
      481 gtccagaaaa aggaatcttc aaaaagctat aaacaaaact acgttctcat cattgatgaa
      541 atcaacagag gaaatgtttc tcagattttc ggtgagctga tcactctgat tgaagaggat
      601 aaacgcattg gaggaaagga agaactggaa gtgactctgc cgtatagcaa ggaatctttc
      661 ggtgttccgt ccaatctcta cattatcggc acaatgaata cggctgacag aagcgtggag
      721 gctctggata ctgctttgag aagaaggttt cattttgagg aaatgatgcc tgatccgaag
      781 ttgattgcgg atgaaggttt ggtgcagaac gggattttgg aaggtattga tctggttaag
      841 cttctgaata caatcaatga aaggattgtg ctcctgctgg acaaggatca tcagatagga
      901 cacagttatt ttatggctgt cgagaatctg gaagatctca aatccgtatt taaaaacaaa
      961 atcattcctc ttcttcagga gtatttttac ggagattacg gaaagatccg tttagttctc
     1021 ggagacaaat ttattgaaaa agcagatggt aaaaatatta cttttgcagt aaaggatgaa
     1081 g
//
LOCUS       DAOQGJ010001849         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118297_length_1081_cov_2.333333, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001849 DAOQGJ010000000
VERSION     DAOQGJ010001849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118297_length_1081_cov_2.333333"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PK683_21985"
     CDS             <1..746
                     /locus_tag="PK683_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11184.1"
                     /translation="QRLLLIQSAVRGRKDYVNILSSMDRQELLSFLFILSQFGDAVRG
                     EAPEEFIYFSSIPYVVEWQKGKYSVPSEILEYLSGEKIFRDQNYIFALLPMLPLKEKK
                     SWIKWLEKDFQGNEKQLNQEIYRELRKLVRKRQLKSIFKEKEIPLDKLWKPGESRFMD
                     WYYKDITTFYFAAQEMEKAETDPLISSLLQEIKCGRWILKKDPEKFQEKTSSRLVATV
                     EGSSFQFRETEFSWEMEHPDTEELLFHGE"
BASE COUNT          353 a          217 c          227 g          284 t
ORIGIN      
        1 agcagaggct tcttctgatt cagtcggctg tcaggggcag aaaagactat gtgaacatac
       61 tgagttcaat ggacagacag gaacttcttt ccttcctttt catactcagt cagttcggtg
      121 atgcagtcag aggcgaagcc cctgaagagt tcatttattt ctcttccatt ccgtatgtgg
      181 ttgaatggca gaaaggaaaa tacagtgtcc cttccgagat tctggaatat ctttccggtg
      241 aaaaaatttt ccgggatcag aattacatat tcgctctgct tccaatgctt cccttgaagg
      301 aaaaaaaatc ctggataaaa tggctggaaa aagactttca gggaaatgaa aagcagctga
      361 atcaggaaat ctacagagaa ctcagaaaac ttgtcagaaa aagacagctg aaatccatat
      421 tcaaggaaaa agaaatccct ctcgataaac tgtggaagcc tggagaatcc agatttatgg
      481 actggtacta taaggacatt actacattct attttgcagc gcaggaaatg gaaaaggcag
      541 aaactgaccc tctgatcagc agcctgcttc aggaaatcaa atgcggaaga tggatactga
      601 agaaagatcc ggaaaaattc caggaaaaaa cgagttccag acttgtagcg actgtggaag
      661 ggtcctcttt tcagttcaga gagactgaat tctcctggga aatggaacat cccgatacag
      721 aggaactgct ctttcacggg gaataattat cagatcctgc ggtgcggctc tgcatattgc
      781 cggacacagg gcaagtaagt catttattca gcatactgta taaggaatga aatataagtt
      841 cacatatatc gttgagtgcc gcctccaatt ttcataaaaa tctttagaca aggacatatt
      901 ctgcactttc tctcggtgca tccgatacag tctcaaaatg ctacactccc atacattcaa
      961 taagttgcct gaacaggctt aaatgaaaat ctgttcatag aaatttactg cttacagagg
     1021 ataatgaaag acttctgctg aatatatgtt cacatatatc cattgcaaaa tatttccgaa
     1081 c
//
LOCUS       DAOQGJ010001850         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118315_length_1081_cov_2.223197, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001850 DAOQGJ010000000
VERSION     DAOQGJ010001850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118315_length_1081_cov_2.223197"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PK683_21990"
     CDS             complement(<1..733)
                     /locus_tag="PK683_21990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH11185.1"
                     /translation="MSETYSDLSKQILESTPMAVLLWDSSGKILDWNFSFQSIFGYSK
                     GSIENISQCSMQEDLEKEMSLLVFYRSRKHKTYTKKIIASDGTVIPCTVMTYPVEDCG
                     LIVAYSSWIKKSSSEVRTLSNDFIRRQNQVLLHLTKSDKADSGDIEQALKETVKSASE
                     ALNCERSSVWVYNSDNTAIECLCLYRKKEDDYTKGVMLHSEDFPSYFRYLSEERFLAA
                     DDAMTDPNTAEFADVYLSPLNIKSML"
BASE COUNT          336 a          231 c          196 g          318 t
ORIGIN      
        1 ccagcattga tttaatgttc aaaggagaaa gatacacatc cgcgaattct gcagtattcg
       61 gatcagtcat tgcgtcatcc gcagcaagaa acctctcttc ggacaaatat cgaaaatagg
      121 acgggaaatc ctctgaatga agcattactc ccttagtata atcatcctct ttttttctgt
      181 aaagacacag acactcaatc gcagtattat ctgaattata cacccagaca cttgaccttt
      241 cacagttcaa tgcttctgat gcagacttta cagtttcctt cagagcctgt tcaatgtctc
      301 cggaatcggc tttatccgac tttgtcagat gcagaagaac ctgattctgt cttcgaataa
      361 aatcatttga cagtgttctg acttctgaag aactcttttt gatccaggaa ctgtacgcaa
      421 cgatcagacc gcagtcctcc acaggatacg tcattactgt gcagggaatc accgttccgt
      481 ctgaagctat aatttttttt gtatacgttt tatgctttcg gctcctgtaa aacacaagca
      541 gggacatttc tttttccaga tcctcctgca tggaacactg agatatattc tcaatgcttc
      601 cttttgaata tccgaagatg gactgaaagc tgaaattcca gtctaatatt tttcctgaac
      661 tgtcccaaag gagaacagcc ataggagtgg actctaaaat ctgttttgaa agatctgaat
      721 atgtttctga catacgtttt cctgaaatag cagcttattt attccatact ctgaaaaatc
      781 tacaagaagt ttttttgaaa aaaagggtca gaccgaaaaa aaacagttga ccttgctgtc
      841 ccttgcttat actaatgccc catgaattcg ttccatactc catggaaaag aaattcataa
      901 tattgtagac tgcattattc cattgaattg tggaaagtta aagcagattt atcaaggaga
      961 actcaattgg gatttaaaga ttttatatca ggcaaaatgg gagccggcta caagccgatg
     1021 aaacctgcct caaacgcaaa agtatacagc aagggcaagg taaagaaact tcctccaaag
     1081 g
//
LOCUS       DAOQGJ010001851         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118456_length_1080_cov_2.951220, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001851 DAOQGJ010000000
VERSION     DAOQGJ010001851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118456_length_1080_cov_2.951220"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..>1080
                     /locus_tag="PK683_21995"
     CDS             98..>1080
                     /locus_tag="PK683_21995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH11186.1"
                     /translation="MSIMATFGFILMCADRYIDGRKKAENSLKESETLFKIIFDGAGV
                     GLTIVDQNGKWISVNEYFLNLLGYTREELELLSIQKITHPDDLDLTELRTKEIMENRI
                     NYFRIEKRYICKNGNVLWVDLSVSARKNEQGKILSLIGAISDISSRKKTEYTLHKVLS
                     EQEIILNNINIGIAKTVNRKQAWINNKLAEMFGYKREEMEGHLTRTLYPSQEVYESVD
                     RKAYPFLSQGNAYIGEQDMIRKDGTVINVRINGNAVEPSDMSKGIIWIIEDITDQKMA
                     REELLASEEKFKSFFEKNKAAFLIINPADGKINDANSSAANYYGYSIEKLKT"
BASE COUNT          387 a          196 c          224 g          273 t
ORIGIN      
        1 caggggcttt tactttcttt tctggaacaa agatccctcc cagtcctatt tcagacagaa
       61 tctcatccag gatatatcat atatatcttt taatgtaatg agcataatgg caactttcgg
      121 attcattctt atgtgtgctg acagatatat tgatggcaga aaaaaagcag agaattctct
      181 gaaagaaagc gaaacacttt tcaaaatcat ctttgacggc gcaggagtcg gactgactat
      241 cgttgatcaa aatggaaaat ggatcagcgt aaatgaatat tttcttaacc tgcttggata
      301 cactcgggaa gaactggaac ttctcagtat tcagaaaatc acacatcctg atgatctgga
      361 tctgacagaa ctcagaacga aagagatcat ggaaaacagg ataaactatt tcaggataga
      421 aaaaagatat atatgcaaaa atggcaatgt cctctgggtt gatctttcag tatcagcaag
      481 aaaaaatgag cagggaaaaa ttctttctct gataggtgcg atcagcgaca tttcctccag
      541 aaaaaaaacg gagtatacgc tgcacaaggt tctttctgaa caggaaatta tattaaataa
      601 tatcaacata ggcattgcga aaacagtgaa ccgaaaacag gcatggatca acaataaact
      661 ggcagaaatg ttcggataca aaagggagga aatggaaggt catctgacaa gaactttata
      721 cccttctcag gaagtttacg aaagtgtaga cagaaaggca tatccttttc tctctcaggg
      781 caatgcctat atcggtgaac aggatatgat ccgaaaagac gggaccgtca tcaatgtcag
      841 aattaacggg aatgcggttg aaccttctga tatgtccaaa ggcatcatct ggatcattga
      901 ggatattacc gatcagaaaa tggcgagaga agaacttctt gccagtgaag aaaagttcaa
      961 gagtttcttt gaaaaaaaca aagccgcatt tctaatcatc aatcctgctg acggaaagat
     1021 aaacgacgca aactcgtctg ctgcaaatta ttacgggtat tcaatagaga aactgaagac
//
LOCUS       DAOQGJ010001852         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118640_length_1079_cov_2.960938, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001852 DAOQGJ010000000
VERSION     DAOQGJ010001852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118640_length_1079_cov_2.960938"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..967
                     /locus_tag="PK683_22000"
     CDS             119..967
                     /locus_tag="PK683_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH11187.1"
                     /translation="MDSRFNHNQTNFIGISEGGIRTSVAVEKYRILFDVGYLPYYYSQ
                     MDNLFLTHGHLDHSAGVPYYISQRSLKKMKGPNIYVPEEMKDDLEQILQLYQKIENFK
                     YSYNLFGLKAGDTVHLSSHAACRAFPTLHRIPSLGYTVYDKSLKLKDELKVLTGDEIR
                     NLRKKGLEIHDHKLTPVISFSGDTQIDPILTNQDVLNSKVLFLECTYLCEERPKEKAR
                     EWGHLHLDEIAEHAELFQNEKLVLMHFSKRYSYSEIRNYIRKKLPEDLCKRTVPLIPE
                     KNIHVY"
BASE COUNT          333 a          215 c          218 g          313 t
ORIGIN      
        1 attttttgca agtgtccggt aaacgatttt aattggaaag aactttttat tttattccgt
       61 aaaaagctag cggattgata aattatggcc gcataaaaat ctgtacctga ggtcattttt
      121 ggacagccgt tttaatcata atcagacaaa ttttatcgga atttcggaag gcgggatcag
      181 aacatctgtg gctgtcgaaa aatacaggat tctgttcgat gtgggttatc tcccctacta
      241 ttattctcag atggacaatc tttttctgac tcacggtcat ctggatcatt ctgcaggagt
      301 gccctattat atttcgcaga gatctctgaa aaaaatgaaa gggccgaata tctatgttcc
      361 tgaggaaatg aaggacgatc tcgagcagat tcttcagctt tatcagaaaa ttgaaaactt
      421 caaatattcc tacaatctgt tcggtctgaa ggcaggggat actgtccatc tcagctcaca
      481 cgccgcctgc agggcgtttc ccacacttca cagaattcct tccttaggat atacagtata
      541 tgataaatcc ttaaaattga aagatgagct caaagtactg acaggcgatg agatacgcaa
      601 tctcaggaaa aaagggctag aaattcatga ccataaactg acacctgtga tcagtttttc
      661 aggagacacg cagattgatc cgattctcac aaatcaggac gtactgaatt cgaaagtgct
      721 ttttctggaa tgcacctatc tctgcgaaga aagaccgaaa gaaaaggcgc gggaatgggg
      781 acatcttcat ttggatgaaa ttgcagaaca cgcagaactg tttcagaatg aaaaacttgt
      841 actgatgcat ttttccaaaa gatactccta ctctgaaatc agaaattata tccgaaaaaa
      901 acttccggaa gatctttgca aaagaactgt tccattgatt ccggagaaga atatacatgt
      961 ttattgattc accttatgcc ttcggctgca gtaaacattt gagcagggga tcttccagtc
     1021 ttcagggctt cgtcctcgct ctttggaaat tgtctaaaaa tcccgttttt actgatcca
//
LOCUS       DAOQGJ010001853         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118652_length_1079_cov_2.801758, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001853 DAOQGJ010000000
VERSION     DAOQGJ010001853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118652_length_1079_cov_2.801758"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1079)
                     /locus_tag="PK683_22005"
     CDS             complement(<1..>1079)
                     /locus_tag="PK683_22005"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNH11188.1"
                     /translation="HSERLGDAFEYLLSVLGSQGDAGQFRTPRHIIDFMVEITDPKKN
                     ETVLDPACGTAGFLISSYKYILKCNTKKKSGDELNTAEKKKLLSNFKGYDISPDMVRL
                     SLVNMYLHGFVQPEIMEYDTLTSEDRWKEYADVILANPPFMSPKGGIKPHKKFSVQSN
                     RSEVLFVDYIAEHLTPTGRAAVIVPEGIIFQSAGAYKQLRKMLVENYLFAVVSLPAGV
                     FNPYSGVKTSILLMDRNLCSKTDSVLFVKIENDGFGLGAQRKEVSGSDLPNAVSVIKK
                     WKNIIHSDKQDTEELNPFLESGFALIAKKDKLAEKEEYNLTGDRYKVTQRAQSTQSWP
                     MVKLGDVCKLGRGFAFKSDDYVSTNG"
BASE COUNT          301 a          273 c          188 g          317 t
ORIGIN      
        1 ccattggtcg aaacgtaatc atcactctta aatgcaaacc cccttcccaa tttacacaca
       61 tcccccagtt ttaccattgg ccagctctgt gtgctctgtg ctctctgtgt gaccttatat
      121 ctatcccctg tcagattgta ttcttccttc tctgccagtt tatccttctt cgcaatcaaa
      181 gcaaatccgc tttccaaaaa cggattcagc tcttctgtat cctgcttatc cgagtgaatg
      241 atattcttcc attttttaat tacacttaca gcattcggaa gatcagaacc ggacacttcc
      301 tttctctgag cacctagacc gaacccgtca ttttcaatct tcacaaacag aacagaatct
      361 gttttgctgc agagatttct gtccatgagg agaatggatg tctttacgcc tgaataggga
      421 ttgaatactc ccgcaggaag agatacaaca gcaaaaagat aattctccac aagcatttta
      481 cgaagctgct tatacgcgcc cgcactctga aaaatgattc cttcaggaac aatgacagcc
      541 gcccgtcctg tcggagtcag atgttccgca atataatcca caaacagaac ctcacttcga
      601 ttactctgaa cagagaattt tttatgaggt ttaattccgc cttttggaga catgaaagga
      661 ggattggcga gaatcacatc cgcatattcc ttccatctgt cctcacttgt caaagtgtca
      721 tactccatga tctccggctg cacaaatcca tgcagataca tattcacaag agaaagcctg
      781 accatatcgg gggatatatc atatccctta aaattggaaa gaagcttttt cttttccgca
      841 gtattcagct catcacctga tttcttttta gtattgcact ttaaaatata tttataagat
      901 gatataagaa agcctgctgt cccgcaggca ggatctagaa ccgtttcatt tttcttagga
      961 tcagtaatct ccaccatgaa gtcaatgata tgtctgggag ttcggaactg tcctgcatcg
     1021 ccctgactac cgagaacaga cagaagatat tcgaatgcat ctcccagccg ttccgaatg
//
LOCUS       DAOQGJ010001854         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118667_length_1079_cov_2.633789, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001854 DAOQGJ010000000
VERSION     DAOQGJ010001854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118667_length_1079_cov_2.633789"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PK683_22010"
     CDS             complement(<1..323)
                     /locus_tag="PK683_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11189.1"
                     /translation="MILLFSVFTEAVRGISSDRADVKEKRSAFLSEPVCLLILVFSFL
                     ISCSGVKQNSAKIENGVLELSAFSIPKEEIVSLEGKWTFFWGKLYSSSEAELKQNESD
                     FFTVPS"
     gene            complement(740..>1079)
                     /locus_tag="PK683_22015"
     CDS             complement(740..>1079)
                     /locus_tag="PK683_22015"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH11190.1"
                     /translation="KFLSHDPAVIEAYDKDPLVHGKISFQMGRNLFHLTSVMNNKAHL
                     IRIPIMMIHGDQDGIADVEGSKEFFTHLQTPKKELKVYPGLYHELMNELPQYRNAVLA
                     DIKKFILSYI"
BASE COUNT          319 a          207 c          226 g          327 t
ORIGIN      
        1 gagggaaccg taaaaaagtc agattcattc tgtttcagtt ccgcctcgga agaactgtaa
       61 agttttcccc agaaaaaagt ccattttcct tcgagtgaaa ctatttcctc tttaggaatg
      121 ctgaatgcgg aaagctccag cacaccgttt tcaatttttg ccgaattctg ctttaccccc
      181 gaacaggaaa tgagaaagga aaagacaagg ataaggagac agaccggttc agaaagaaaa
      241 gcggaccgtt tttccttcac atctgccctg tccgatgaaa ttcctctgac tgcttccgtg
      301 aagactgaga atagtaaaat catatataat agaggatttt tatttaaagg gctttcgaaa
      361 gaagataaat ctgtcttcct ttctgcaggt atggaagcct taggcaaact gtctgatgaa
      421 aatgcatacc tgaacagaag catctgtgaa atcctcgtac tcgatgatca gcattcaggt
      481 atgaaagtaa agaataattt ctgtttttta aggcagtctg agatttctga agagatttta
      541 gatgattctt tagggcttcc cctaatgttt tttgatttat tgagcagaaa atatttcgaa
      601 taaccctgct tttgtcccct ctgcccgact tatgaaatct ttgcataaag tgaatgggac
      661 atataagaca tttcggcggc ttctatactg caggaaagag cttcattgca atgtgaaagc
      721 cgccgaacag agatgttttt cagatatagc tcagaataaa tttcttgata tccgcgagaa
      781 cggcatttct gtactgaggc agttcattca tcagttcatg atacagtcca ggatatactt
      841 tcagttcctt ttttggagtc tgaagatgtg taaaaaattc ttttgaccct tctacgtcag
      901 caattccatc ctgatccccg tgaatcatca taattggaat gcgtatcaga tgagccttgt
      961 tgttcataac ggaggtgagg tgaaaaagat ttcttcccat ttggaaggaa attttgccgt
     1021 gcaccagagg gtccttgtca tatgcttcaa tgactgcggg gtcatgagaa agaaatttt
//
LOCUS       DAOQGJ010001855         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118698_length_1079_cov_2.324219, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001855 DAOQGJ010000000
VERSION     DAOQGJ010001855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118698_length_1079_cov_2.324219"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PK683_22020"
     CDS             <1..1007
                     /locus_tag="PK683_22020"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNH11191.1"
                     /translation="HTVFLPEHGLFAELQDQVSGSGLKYSLSGWNIVNLYGDSEDTLT
                     ASRDSLRNLDLLLIDIRDVGARYYTFLTTAFYMLQSVSEMKKNGENVPDIIAVDYPNP
                     IGKKAEGSPLDAKYESFVGVRTALHRHGMSVFELLDYYRKKFSLDCSLDFLRPSENTD
                     HYWVPPSPNIPTKDTCFVYPGQCLLEGTNLSEGRGTTRPFEMFGAPFINPFDDLLLHE
                     LNRENKGYYYLRPLFFQPVFHKHAGRVCGGYQIIIKNHKKFHSLLFTLHLLRTLKDYY
                     STKFEYLQGPYEFRSDRPAVELLAGDDLLVSYLYGKGKYSEIREYLNSEEKKWEKERG
                     "
BASE COUNT          307 a          231 c          246 g          295 t
ORIGIN      
        1 ttcatactgt gtttctgccg gaacacggac tgtttgcaga gctgcaggat caggtttcag
       61 gaagcggtct gaaatattct ctttccggat ggaatatagt gaatctgtac ggagattcgg
      121 aagatacact gactgcatcc agagactctc ttcgaaatct ggatcttctc ctcattgaca
      181 ttcgggatgt aggtgcaagg tactatacat tcctcaccac tgcattttat atgctccagt
      241 ccgtgagtga aatgaaaaaa aacggcgaaa acgttccgga catcattgct gtggactatc
      301 cgaatcctat aggaaaaaaa gcggaaggat ctcctctcga tgcaaaatat gagtcatttg
      361 taggagtgag aactgcactt cacagacacg gaatgtccgt ctttgagctt ctcgattact
      421 acaggaaaaa attcagtctg gactgctccc tggattttct ccgtccttct gaaaatacgg
      481 atcattactg ggtgcctcct tctcctaaca tcccgacaaa ggacacctgt tttgtctatc
      541 cgggacagtg tctcctggaa ggaacgaatc tctccgaagg cagagggact acgagaccct
      601 ttgagatgtt cggcgctcct ttcatcaatc cttttgatga tcttcttctg cacgaactga
      661 acagagaaaa taaaggatat tattatctgc gtcctttatt ttttcagccg gtttttcaca
      721 aacatgcagg ccgagtctgt ggaggatatc agatcattat aaagaatcat aaaaagtttc
      781 attctcttct ttttactctt catcttctca gaacactgaa ggattattac agcacaaagt
      841 ttgagtatct tcagggtcct tacgaattcc gttccgacag accggcagtt gaacttctgg
      901 caggagatga tcttcttgta tcttatctgt acggaaaagg aaaatattcc gagatcagag
      961 agtatctgaa ttcagaagaa aaaaaatggg aaaaggagag aggctgaaaa aaaagcagcg
     1021 ttaagcggcg caagcagcga cagcgatcgc gagcgcgaga ggtgcgagtg tccggtatg
//
LOCUS       DAOQGJ010001856         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118704_length_1079_cov_2.262695, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001856 DAOQGJ010000000
VERSION     DAOQGJ010001856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118704_length_1079_cov_2.262695"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK683_22025"
     CDS             complement(<1..438)
                     /locus_tag="PK683_22025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNH11192.1"
                     /translation="MLELDLQSEEKLRKIWSENNFSLDIPIIDLQHIWLIWLLLQMEE
                     EVLQPESPESSSRTDYIISELVSFTTEHFWLEECLMEHTNFGENREHTSQHNRFIEEV
                     SRQILGKSENRKEQILQLIGFLKNWLFGHILIEDSKYKKFFVKE"
     gene            600..1019
                     /locus_tag="PK683_22030"
     CDS             600..1019
                     /locus_tag="PK683_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11193.1"
                     /translation="MSALLIPAIISASMYYDAGFSNLENTYLAGICECNHGSKFEKHV
                     SKNNPDDFILSKDNVNSDTHLNKLPDCHTAKSEKHLCMCKKSSKAKSGQGFAKQIFCL
                     AYSKVFPNVIIDKGFTAEYRVRLRDGFDPLFLIPPES"
BASE COUNT          309 a          227 c          185 g          358 t
ORIGIN      
        1 ctctttcaca aagaattttt tgtactttga atcttcaatc aggatatgcc cgaacagcca
       61 gtttttaagg aacccgatga gctgcaggat ctgttctttt ctgttctccg attttcctaa
      121 aatctgtctg gaaacttctt caatgaatct gttatgctgt gaagtgtgtt ctctgttttc
      181 accaaagttt gtatgttcca tcagacattc ttccagccag aaatgttccg tagtaaagct
      241 cacaagttct gagatgatgt aatctgttct ggatgaagac tctggggatt caggctgaag
      301 aacttcttct tccatttgaa gcagaagcca tatcagccag atatgctgaa gatcaataat
      361 tggaatgtcc aatgagaaat tgttctcact ccagattttt ctcagctttt cttctgactg
      421 taaatcaagt tcaagcatct gtttttacat aaattttttt taacagtttt tttcctagga
      481 aattaaattt tatttatgct tacaatttga cagaattcca attctgcgaa aacgcagtaa
      541 aaatatcgac ttgacgtaca gagccggtct ttttcctttg gaacagtatg ctgaagtttt
      601 tgagtgcact ccttattcct gcaatcattt cagcctccat gtactacgat gccggttttt
      661 ccaatctgga aaatacgtat ctcgcaggta tctgtgaatg caatcacggt tctaaatttg
      721 agaaacacgt ctcaaagaac aatccggatg atttcatcct gtccaaggat aatgtaaact
      781 cagacactca tctaaataaa ctgcctgact gtcacactgc aaaatcagag aaacatctct
      841 gtatgtgcaa gaaatcatcc aaagcaaagt cagggcaggg tttcgcgaaa cagatattct
      901 gccttgccta ttccaaagtt tttcccaacg ttatcattga caaaggtttc accgcagaat
      961 acagagttcg tttaagagat ggatttgatc cactgttcct aatccctcct gaatcataaa
     1021 atttttcctt cagttctgac ataactttaa actgtccgga ttactgtatc cgcatactt
//
LOCUS       DAOQGJ010001857         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_118857_length_1078_cov_3.517107, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001857 DAOQGJ010000000
VERSION     DAOQGJ010001857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118857_length_1078_cov_3.517107"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK683_22035"
     CDS             complement(<1..991)
                     /locus_tag="PK683_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNH11194.1"
                     /translation="MSDNPYSKTVILPKTDFDMKASLKDREPAQMEIWKKDRIFQKMK
                     EKRKNSPRFVLHDGPPYANGNFHVGHALNKILKDFIVKSRHLAGFHADIVPGWDCHGL
                     PIEVQVLKDLGKEASKIGPSELRKKCREYAAKYVGIQGSDFSRFLCFWDEESRYLTMD
                     PSFEAEIVRTFGTLFEKGYIYKGKKPVYWCIQLGTAHAEAEIEYQDHVSPSVYVKFPV
                     IGKDRLFCLIWTTTPWTLPANLAVCFNEEISYSIFESEGDRLILADGLAESVEKATGR
                     TLSKKEKITHDEIRSLKFQHPFLDRISIPLFGSHVTLDAGTGCAYGLPMQLQAE"
BASE COUNT          272 a          251 c          208 g          347 t
ORIGIN      
        1 gctcggcctg tagctgcatt gggagtccgt atgcgcatcc tgttcctgca tcgagagtca
       61 catggctccc gaacagagga atggaaatcc tgtctaagaa tggatgctga aacttaagac
      121 tgcggatctc atcatgagtg attttttctt tttttgaaag agtcctgcct gtcgcctttt
      181 ccacagattc agccagaccg tcagcaagaa tcagcctgtc tccttcggat tcaaaaattg
      241 aataagatat ttcttcatta aaacatactg caagattggc aggaagtgtc cagggagttg
      301 tagtccagat cagacagaac agtctgtcct ttccaattac aggaaacttg acatagactg
      361 aaggggatac atgatcctga tattcaattt cagcttccgc atgagcagtt ccaagctgga
      421 tgcaccagta aaccggtttt tttcctttgt agatataacc tttctcaaaa agagttccaa
      481 aggttctgac aatctccgct tcaaaggaag gatccatggt cagatacctg ctttcctcat
      541 cccagaaaca cagaaatctg gaaaaatcgc ttccctgaat tcctacgtat ttcgctgcgt
      601 attccctgca tttctttctc agttcagacg ggccgatttt tgatgcttct tttcccaggt
      661 ctttcagaac ctgcacttct atcggcagcc cgtgacagtc ccagccggga actatatcag
      721 catgaaatcc tgccaagtgt cttgacttta caatgaagtc tttcaaaatt ttgttcagag
      781 catggcctac atgaaaattc ccgtttgcat aaggaggtcc gtcatgcagt acaaatctgg
      841 gtgaattttt tcttttttcc ttcatcttct gaaagattct gtctttcttc caaatctcca
      901 tctgtgccgg ttccctgtct ttcagggagg ctttcatatc aaaatctgtt ttaggaagga
      961 ttactgtttt tgaataagga ttatcgctca tcttgtcttt ttctcattcc aaattttaaa
     1021 acccttgggt tttctccaat gatttttcaa cagagactct gttttatttt cctgcgca
//
LOCUS       DAOQGJ010001858         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119048_length_1077_cov_4.247554, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001858 DAOQGJ010000000
VERSION     DAOQGJ010001858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119048_length_1077_cov_4.247554"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..408)
                     /locus_tag="PK683_22040"
     CDS             complement(43..408)
                     /locus_tag="PK683_22040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNH11195.1"
                     /translation="MTYSGEDRRKEPRIHFERFGKITDKENQDLKCYIENISTGGAQI
                     FCNRLLVPGSLVVLKILDIPLQELTIPAVVLSSEPMARRRKQILQIAKNLHVENLAHL
                     QFEQKLDSSVLRTITEAAA"
     gene            complement(508..885)
                     /locus_tag="PK683_22045"
     CDS             complement(508..885)
                     /locus_tag="PK683_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11196.1"
                     /translation="MEIMKWLFEKFRNKEMSDDEVFEYMRENHLLMEMSAADFLIDNL
                     NSVRNSSMPFQCLVDTCQRAYLNYLSVGGASEEGGDSFLNYVNWCIRKGTAAGKRLKD
                     LNRKEQDEFWNLVNEISEQLKDI"
BASE COUNT          304 a          235 c          182 g          356 t
ORIGIN      
        1 tttccttgat aaaatcaggt atgccttctg acagaatttc tttcatgctg cggcctctgt
       61 aattgtcctg agaacagaac tgtccagctt ctgttcaaac tgaagatggg caagattttc
      121 cacatgaagg ttctttgcga tctgaagaat ctgctttctt cttctcgcca taggctctga
      181 actcagaact actgcaggaa tggtcagctc ctgaagcgga atgtccagta ttttcagcac
      241 aacaaggctg cctggaacaa gaagcctgtt gcagaaaatc tgagcaccgc cggtgctgat
      301 attctcaata tagcatttca gatcctgatt ctctttatca gttattttac cgaatctttc
      361 gaaatgaatt ctcggttcct ttcttctgtc ttcgccgctg taagtcactt acaattccct
      421 tataggaaag agatatgagg aaagcaaaat atgtcaagga tatttatcag agcacatcct
      481 gtgatctgca gaagaaaacc cgctttttta tatgtctttt aattgttctg aaatctcatt
      541 cacaaggttc cagaattcgt cctgttcctt tctgtttaga tctttcaatc tttttccagc
      601 agcagttccc tttcgaatac accaatttac ataatttaaa aagctgtcac cgccttcttc
      661 agaggctcct ccaactgaaa gataatttaa atatgccctt tgacaagtgt ctaccaggca
      721 ctgaaaaggc atggaagaat ttcgaacaga attgagatta tctatcaaaa agtctgcagc
      781 agacatttcc atcaaaagat gattttcccg catatattca aatacttcat catcactcat
      841 ttctttattt ctgaattttt caaaaagcca cttcataatt tccatttttc caataccatt
      901 ctctgtcaga ctgatatcaa gggggatttt cagccaggaa taatcctgac tgcactaaga
      961 agacaatttc gaatttctct gaactccagg aattaatctc agtttgatgt ctcgataggt
     1021 ttgtttttct aattgagata tttttactac atcatttcct gatctcggag gaactgg
//
LOCUS       DAOQGJ010001859         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119051_length_1077_cov_3.887476, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001859 DAOQGJ010000000
VERSION     DAOQGJ010001859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119051_length_1077_cov_3.887476"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>1077
                     /locus_tag="PK683_22050"
     CDS             59..>1077
                     /locus_tag="PK683_22050"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNH11197.1"
                     /translation="MSDLEKIFPAVVKINTSEGSGSGLYYKANDVIITNFHVVSGFKK
                     VGVETQSKETIAADVLVVNPLNDLAILKPHKVLDMPDIGFLRHQNLKAMDKVSVLGFP
                     YGMPFTVTEGIVSNVKQVLSGQSYIQTDAAVNPGNSGGPVANHRGEIIGITTSKFTNA
                     DNMGFALPADVVLEELDALRLNPNLAFAVKCTSCNHPLYEEVEHCPNCGAKLDIKTLF
                     KEAPKTEIAAFVEDVFKELKLDPVVARKGFEFWEFHQGSALVRYFIYRNNYLFAVSPL
                     VKLPKTNLEGIYTHILSSPAKPFYLAIRDSIVYISYRAHLSDMKGPNKKEIQDNLVKL
                     AVKAD"
BASE COUNT          331 a          224 c          233 g          289 t
ORIGIN      
        1 catcctcagc aaagagcatt ttctctaaaa ttagcagtct ttttaaagga taatcacaat
       61 gagcgattta gaaaaaatat ttccggcagt cgtaaaaatc aatacttccg aaggaagcgg
      121 aagcggactt tactataagg ccaatgatgt aattatcacg aacttccatg tcgtgagcgg
      181 atttaagaaa gtgggagtgg aaactcagtc caaagaaacg attgcagcag acgtgctcgt
      241 ggtaaatcct ctgaacgatc tggctattct gaagcctcac aaggttctgg atatgcctga
      301 tatcggcttc ttaagacatc agaacctcaa ggcgatggat aaggtatcag ttctaggttt
      361 tccctacggg atgcctttca ctgtaactga aggcattgta tccaacgtta aacaggtatt
      421 gagcggtcag tcttacattc agactgatgc tgcagtgaat cctggaaaca gcggaggacc
      481 cgttgcaaac cacagaggcg aaatcatcgg gattacaaca tccaagttta ccaatgcgga
      541 caatatgggc tttgcactgc ctgctgacgt tgttctggag gaactggatg cactccgtct
      601 gaatccaaac cttgcatttg cagtgaaatg tacttcatgc aaccaccctc tttatgaaga
      661 agtggaacac tgccctaact gcggtgcaaa actcgacatc aagactcttt tcaaggaagc
      721 tccaaaaact gaaatcgcag catttgtcga agatgttttc aaggaactga agctggatcc
      781 tgtagttgca agaaagggat ttgaattctg ggaattccat cagggaagtg cgcttgtgag
      841 atattttata tacagaaata attatctctt tgcagtaagt cctctggtta agcttcctaa
      901 gacaaatctg gaaggaatct atactcatat tctttcaagt cctgctaaac cgttttatct
      961 ggcaatacgt gactccattg tctatatttc ctaccgtgcg catctttcag atatgaaagg
     1021 tccgaacaaa aaagaaattc aggacaacct tgtaaaactt gcagtaaaag cggatga
//
LOCUS       DAOQGJ010001860         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119258_length_1076_cov_2.995103, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001860 DAOQGJ010000000
VERSION     DAOQGJ010001860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119258_length_1076_cov_2.995103"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PK683_22055"
     CDS             complement(<1..272)
                     /locus_tag="PK683_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11198.1"
                     /translation="MKKGYILFTAYSIAVLLNIFSCSDSDKKADPKSTAAIAILGQQK
                     SASTAAASSSPSSSEAAPGDNALAKGTFTVNKYFGASPFVLNSTYTT"
     gene            complement(283..>1076)
                     /locus_tag="PK683_22060"
     CDS             complement(283..>1076)
                     /locus_tag="PK683_22060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallo-mystery pair system four-Cys motif
                     protein"
                     /protein_id="HNH11199.1"
                     /translation="NLLLLAALPKATSLNFSTVVGSQKFACGTAYTVNGTANVKFRDF
                     RFFVTDVKLISSDNTEIPFTLNTDNVWQSNGVALLDFETGSCGSTPSMGSGSAETNST
                     VTGTVPAGSPSSYKGVKFSISVPTAINFQDSTTQPAPLNVNAMYWSWTSGYKFAKIEF
                     SNDDFSANITNLHLGSTSCVNKVCSKSYRPTISISKSSGTYNPSADTVTFDISAFLSG
                     YAGYSSENSCMPGTNSNCLKIHNALGLDSEGNVSTSLTQTAFSIK"
BASE COUNT          285 a          210 c          268 g          313 t
ORIGIN      
        1 gtggtgtaag ttgaattcag tacgaacgga gaggctccaa aatatttatt gaccgtaaat
       61 gtacccttgg caagtgcatt gtccccaggg gcggcttctg aagaagaggg tgaactggaa
      121 gccgctgcag tggatgcaga tttctgctga ccaaggatag caattgcagc agtacttttc
      181 ggatctgctt ttttatcact gtctgaacag gaaaaaatgt tcagcagaac ggcgattgaa
      241 tacgcggtaa ataaaatata tccttttttc attttgttct ccttatttga tgctgaatgc
      301 agtctgggta agtgaagttg aaacatttcc ttccgagtca agtccaagag cgttgtgaat
      361 cttcagacag tttgagtttg ttccaggcat acagctgttc tcagaactgt atcctgcgta
      421 accgctcagg aaagcactga tgtcgaatgt gaccgtgtct gcagaaggat tgtaagtccc
      481 ggatgatttt gaaatcgaga ttgtcggtct gtagcttttg gagcagactt tgtttacaca
      541 gcttgtgctt cctaaatgca gatttgtgat gtttgcagag aagtcgtcat tcgaaaattc
      601 gatctttgca aatttatatc cggatgtcca tgaccagtac atcgcattta cattcagggg
      661 tgcaggctga gtagtggaat cctgaaaatt gattgctgta ggtacactga tggagaattt
      721 tactccctta taggaagagg gcgaacctgc aggaacagtt ccagtcactg tgctgtttgt
      781 ttctgcggag ccgcttccca tagaaggcgt tgaaccgcag cttcctgttt caaaatccag
      841 aagagccact ccgtttgact gccatacatt gtcggtgttg agagtgaaag gaatttcagt
      901 attgtcagag ctgatcagtt taacgtctgt tacgaaaaat cggaaatccc tgaattttac
      961 atttgcagtg ccgtttactg tgtaagctgt tccgcaggca aatttctgag aacctacaac
     1021 agtgctgaag ttcagagaag tcgccttagg cagagcagca agaaggagca gattcc
//
LOCUS       DAOQGJ010001861         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119462_length_1075_cov_2.995098, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001861 DAOQGJ010000000
VERSION     DAOQGJ010001861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119462_length_1075_cov_2.995098"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..459)
                     /locus_tag="PK683_22065"
     CDS             complement(16..459)
                     /locus_tag="PK683_22065"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH11200.1"
                     /translation="MFKIEVISENVSFQEIFVSSEGSLDSFSCADFYDFIIAENAKGF
                     KRIILDFSKIDYITSAGLSVLIHLQNRFQSENSILILTGLNSEIQQMFHFFGLNKVFV
                     TALSREGAEEILESMFFMKKTPSVEGKEVIARNRFIFEDVPEEKK"
     gene            complement(465..>1075)
                     /locus_tag="PK683_22070"
     CDS             complement(465..>1075)
                     /locus_tag="PK683_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11201.1"
                     /translation="LPIPQSFGKEFEGEGGFLDKWILKNFPDIKYQTILLSAPIYHVE
                     RLEALGTIHMVYNRGNLLFFMKNQKDVMKWIVLNFGLVALISTILTWSVAAYFMIGSY
                     KQGVSTGKGDTVSTTKLGSLLQKQEETLQRMITPMPVKLDSDISLNIMDQERDLSDST
                     DYQAQFLYQTEQAPAVTSEEEKKKAAEKNTSLTEAVDAIPLD"
BASE COUNT          307 a          233 c          209 g          326 t
ORIGIN      
        1 ctgagaaatt caggttcatt ttttttcttc cgggacatcc tcaaatataa acctgttcct
       61 ggcaatgacc tcttttcctt caactgaagg agtctttttc ataaagaaca tagattcaag
      121 gatttcttca gccccttccc tggacagtgc agtgacaaat accttgttca gtccgaagaa
      181 atgaaacatc tgctggattt ctgaattcag tcctgtaaga atgagaattg aattttccga
      241 ctgaaacctg ttttgaagat gaatcagaac agaaagccct gccgatgtga tatagtcaat
      301 tttcgaaaaa tccaggatga ttcttttgaa tccttttgca ttctctgcaa tgatgaaatc
      361 ataaaaatct gcacaggaaa aagagtccag agatccttca gaggatacga atatctcctg
      421 aaaggaaaca ttttccgaaa tgacttcgat tttgaacatt cagatcagtc taaaggaatt
      481 gcgtcaacag cctcagtcag actggtattt ttttctgcgg cctttttctt ttcctcttca
      541 gaagtgacag caggagcctg ttctgtctgg tacagaaact gcgcctggta atccgtagaa
      601 tcggaaagat ccctttcctg atccataata ttcaatgata tgtctgaatc cagtttcaca
      661 ggcatagggg taatcattct ctgaagagtt tcttcctgtt tctgcagaag agaaccgagt
      721 ttggtagtgg acacagtgtc cccttttcct gtgctgactc cctgcttata gctgcctatc
      781 ataaagtatg ctgcaactga ccaagtgaga atagtagaga tcagtgcaac cagtccgaaa
      841 ttgagaacaa tccacttcat tacgtctttc tggtttttca taaaaaagag cagatttccc
      901 ctgttgtaga ccatatgaat cgtgcccaat gcctccagcc tctccacatg ataaattgga
      961 gccgaaagca gaattgtctg gtatttgatg tctgggaaat ttttcagaat ccatttgtcc
     1021 agaaatccgc cctcaccttc aaattctttt ccaaaggatt gaggaatcgg gagct
//
LOCUS       DAOQGJ010001862         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119488_length_1075_cov_2.594118, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001862 DAOQGJ010000000
VERSION     DAOQGJ010001862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119488_length_1075_cov_2.594118"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1075)
                     /locus_tag="PK683_22075"
     CDS             complement(<1..>1075)
                     /locus_tag="PK683_22075"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNH11202.1"
                     /translation="NKIRSSTDRFLHFNIDQGLKDIPWELLYDGREFLADKFFIGRTV
                     KGAARQFKEEGTKKMKMLIIADPTEDLEWAQKEGETLFQVLREKISSNNLELQFVAGR
                     QITKLKLLSMIKGKNIIHYAGHLFFSEDALENGWLLSDGKVLKAREISNSGTSASLVF
                     SNSCQSSRSAESSPSIMSYFAGSFLLSGIKCFVGTNWEIADNERTLDFTIRFYQTLFQ
                     HKSVGEALYFAREFARRNYESWDLTWANYTLHGSPALLMSPKGSESRSKASSFDMIQY
                     AYPMPIAKSFMKFVSEDKSGSDALVLLKLLAKAFEDFSKMTGAIIFSDHSRQSLGKIS
                     IPENQEIGIRKWWELIYAAMWDFK"
BASE COUNT          301 a          241 c          214 g          319 t
ORIGIN      
        1 tcttgaaatc ccacatagcc gcatagatga gttcccacca ttttcgaatt ccaatctcct
       61 gattttcggg aatggaaatt ttcccaagtg actgcctgga atgatcgctg aagataatgg
      121 ctccagtcat tttagaaaag tcttcaaatg cctttgcaag aagtttaaga agaaccaaag
      181 catcggatcc tgacttgtcc tccgatacaa atttcataaa tgactttgca ataggcatcg
      241 ggtaagcata ctgaatcata tcaaaactgg aagctttgct tcttgattca gaacctttgg
      301 gagacatcag aagtgcagga gagccgtgaa gtgtataatt tgcccaagtc agatcccagg
      361 attcataatt tcttctggca aattctctgg caaagtacaa tgcctcaccg acagatttgt
      421 gctgaaacag agtctgataa aatcgaatcg taaaatcgag agttctttcg ttgtctgcga
      481 tctcccagtt tgttcctaca aaacatttaa tgcctgaaag gaggaatgaa ccggcaaaat
      541 aactcataat cgacggactg ctttctgcag atctggatga ctgacaggaa ttgctgaata
      601 ccagactggc ggacgtgccg ctgttactga tttccctcgc tttcagaacc tttccgtcag
      661 acagaagcca gccgttttcc aatgcatctt cggaaaagaa gagatggcct gcataatgta
      721 taatattttt ccctttaatc atcgaaagaa gtttaagttt ggtaatctgt cttcctgcca
      781 cgaactgcag ttcaagatta ttggaggaga ttttttcgcg gagcacttga aacagtgtct
      841 ccccttcctt ctgtgcccat tccagatctt ccgtgggatc cgcgataatg agcattttca
      901 tttttttagt tccttcttcc ttgaactgcc ttgctgcccc ttttacagtc ctgccgatga
      961 aaaatttatc agctagaaat tctcttccgt cataaaggag ttcccacgga atgtccttca
     1021 ggccctgatc aatattgaaa tgcagaaatc tgtccgtaga agaacgaatt ttatt
//
LOCUS       DAOQGJ010001863         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119517_length_1075_cov_2.337255, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001863 DAOQGJ010000000
VERSION     DAOQGJ010001863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119517_length_1075_cov_2.337255"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1075)
                     /locus_tag="PK683_22080"
     CDS             complement(<1..>1075)
                     /locus_tag="PK683_22080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNH11203.1"
                     /translation="KLQEVILYRNQALVMRTLEVDLKDGIHEIIVSRLPSEIIQSSLY
                     AESSGAEIRGTRMKTKELSEDPEAPVKELDEKIEKISSDISKVNRLLQVNQSKVLYLT
                     KQEDFAAATEKQEMTKGVLNPDVIAKITLFHFDQKRELANEEFQLQADLKKLNKEKDH
                     LQRQRKQLTKTTPRLLEASVFVEKTGQGTAQIKLFYLVQNAGWNPVYNIYSKSGEKNV
                     TTEFNAKIQQFSGENWENVQITLSNATPALSAAAPSLSPFRLSLSSGPSGYVTGDLRA
                     ATQNAAKKMSIATQKQMGAQNWDESAESGWAMNSAANEFQNLEIMAKDEDLSLLKKEL
                     KGEQNSPSVSFPLKGKLSLSSKNE"
BASE COUNT          222 a          258 c          223 g          372 t
ORIGIN      
        1 gttcattctt gctcgaaaga ctcagttttc ctttgagagg aaagcttaca ctgggagaat
       61 tctgctctcc tttcagttct tttttcagaa gactcaggtc ttcatcctta gccataatct
      121 ccaggttctg aaattcattt gctgcactgt tcattgccca tccgctttct gcagactcat
      181 cccaattctg tgctcccatc tgtttctgag tagcgatgct catctttttg gcagcattct
      241 gagttgccgc tctcagatcg ccggtcacat acccggaagg ccctgacgac agactgagcc
      301 ggaacgggga cagagaaggg gctgctgcac tcagagccgg agttgcgttg gaaagagtga
      361 tctgtacatt ttcccagttt tcaccgctga actgctggat ttttgcatta aattctgtgg
      421 tgacattttt ttcaccggat tttgaatata tattatatac ggggttccag cctgcattct
      481 gtacaagata aaacagcttg atctgtgctg ttccctgacc tgtcttctct acaaatacgc
      541 ttgcttccag aagtctgggc gttgtcttgg tcagctgttt tctctgcctc tgcagatggt
      601 ccttttcctt gttcagcttt ttcagatcag cctggagctg aaattcttca tttgccagtt
      661 ctcttttctg atcaaaatgg aacagcgtaa ttttagcgat tacatccgga ttcagtactc
      721 cctttgtcat ttcctgtttt tctgtagcag ctgcaaagtc ttcctgcttt gtcaggtaaa
      781 gcactttgga ctggtttacc tgaagcagtc tgtttacttt ggatatgtcg gaactgattt
      841 tctctatttt ttcatccagt tctttgacag gcgcctcagg atcctccgaa agctccttgg
      901 ttttcattct agtgcctctg atttctgccc ccgagctttc ggcataaaga ctgctctgta
      961 taatttcgga aggcagtctg cttactatga tttcatggat accgtctttc agatctacct
     1021 caagagttct cattacaaga gcctgatttc tgtagagaat gacttcctga agttt
//
LOCUS       DAOQGJ010001864         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119691_length_1074_cov_2.637880, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001864 DAOQGJ010000000
VERSION     DAOQGJ010001864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119691_length_1074_cov_2.637880"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1074)
                     /locus_tag="PK683_22085"
     CDS             complement(<1..>1074)
                     /locus_tag="PK683_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH11204.1"
                     /translation="KFLNWGFERKRLVGIYAVTLVVISISLGFTLGAEFIPRIDEGEI
                     NIDVRRLPSASINYSRDLNYEIERVIEKNFPEVTSVVTKVGRGESAAEPVGTDEGEMY
                     VKLKDKKDWVSAHDREELMSIMKDKILSTVPSSYISMSQPIENRVNSLLAGSKADIVV
                     KIYGDDLVKLKKLGDQFGEVLSKVEGTGDLRVQRVLGLPLLEIKANREKMARYGVSTE
                     EILDIVKTLRVGYDTGKVFEGLKRYDLIVRLDVDVTDEDQIENVPVMTSSGHEVPLVL
                     VADIKRIEGPASITREGLKRRLFVEINIRGRDLVSYINEAQEKTKSIVESLPEGYEVE
                     WGGQFQNFTRARDRLLLVVPVALV"
BASE COUNT          269 a          294 c          193 g          318 t
ORIGIN      
        1 acaagtgcga caggtacaac cagaagaagt ctgtctcttg cacgggtaaa attctgaaac
       61 tgtcctcccc attccacctc ataaccttca ggaagcgact ctacgattga ttttgtcttt
      121 tcctgtgcct cgtttatgta gcttacaaga tcccgtccac gaatattgat ctcgacaaag
      181 agtcttctct tgagtccctc ccgtgtaatg gaagccggac cctctattct tttaatgtca
      241 gcaactaaaa cgagaggaac ctcgtgcccg ctcgatgtca tgacaggaac attttcaatc
      301 tgatcctcgt cagtcacatc cacatccaga cgcacaatca gatcatatct tttcagtccc
      361 tcgaacactt tccccgtatc gtagcctact cttaatgttt ttacaatatc cagtatctct
      421 tctgtagata ctccgtatct tgccattttt tccctgtttg ccttgatttc aaggagagga
      481 agtcccagca ctctctgaac ccgaaggtcg cctgtccctt ctaccttgga aagaacctcg
      541 ccgaactgat ccccaagttt cttaagcttg accagatcgt caccgtaaat tttcacaacg
      601 atgtcggcct ttgaacctgc cagcagcgaa ttgaccctgt tttcaatggg ctgggacata
      661 ctgatatagg aggacggcac agtggaaaga atcttgtcct tcatgatgct catcagttcc
      721 tcccggtcat gtgcactcac ccagtctttt ttgtccttga gtttgacata catctctccc
      781 tcgtctgttc cgacaggctc agctgcagat tctccccgtc ctacctttgt gacaacactg
      841 gttacttcag gaaaattctt ttcgatgact ctttcaattt cataattcag atctctggaa
      901 taattgatgg aagcggaagg aagccttctc acatcaatgt tgatttcccc ttcgtcgatc
      961 ctcggaataa attctgcccc cagagtaaat ccgagagaaa tagaaatcac tacaagagtg
     1021 actgcataaa ttcctaccag tcttttccgt tcaaatcccc agttcagaaa ttta
//
LOCUS       DAOQGJ010001865         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119894_length_1073_cov_3.011788, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001865 DAOQGJ010000000
VERSION     DAOQGJ010001865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119894_length_1073_cov_3.011788"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PK683_22090"
     CDS             complement(<1..863)
                     /locus_tag="PK683_22090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH11205.1"
                     /translation="MKVAIIHDWLTGMRGGEVVLDSLLKIYPEAELFTLIYNKGKLNE
                     RIENRKIHTAFTDRLPFKASKYRSFLPLFPTAIETFDLRGFDLIISSSHCVAKGIIPP
                     PNARHITYVHSPMRYVWDLYYDYFPRDRGLKFFLLQMISNYLRLWDVSSSSRVDLFTC
                     NSKFIAKRIEKYYRRKSEVVYPPCLPEGFQATETAKDDYYLVVSAFAPYKRIDLAVEA
                     FRKNGRRLVIIGSGQDEKKLKKNAPKNVEFLSHLPTEQVVQFMKKAKAFIFPGLEDFG
                     IAPVEVQGYCTP"
BASE COUNT          284 a          258 c          235 g          296 t
ORIGIN      
        1 ggagtacagt acccctgcac ctccaccgga gcaatgccga aatcctccag tccgggaaaa
       61 atgaacgcct ttgccttttt cataaactga actacctgtt cggtcgggag gtgagaaagg
      121 aattccacat tttttggggc gttttttttc agttttttct catcctgtcc gcttccgatg
      181 attaccagtc tccgcccgtt cttccggaat gcttctacag ccagatcaat tcttttgtat
      241 ggggcaaatg ccgataccac cagatagtag tcgtccttgg cggtttccgt cgcctgaaaa
      301 ccctccggca gacagggggg atataccact tcagatttcc gcctgtagta tttctcgatt
      361 ctttttgcaa tgaacttgga attgcaggta aacagatcca ccctggacga ggagctgaca
      421 tcccagagtc tcagataatt ggaaatcatc tgcagaagga aaaatttcag tccccggtcc
      481 ctcggaaaat aatcataata cagatcccag acgtatctca taggggaatg cacataggtg
      541 atatgtcttg cattcggagg agggatgatc ccttttgcca cgcagtgaga ggaggaaatg
      601 atgagatcaa atcctctcag atcaaatgtc tcaattgcag tcgggaaaag aggcagaaag
      661 gatctgtact tggaagcctt gaaaggaagc ctgtcggtaa aagcagtatg gatcttcctg
      721 ttttcaatcc tttcattcag ctttccctta ttgtagatca gtgtaaaaag ctccgcttcg
      781 ggataaattt tcagaagaga gtccagtaca acctcccctc ctctcattcc agtcagccag
      841 tcgtgaatga ttgccacttt cataataaca gttctctcag ccattcagaa tgatgctgga
      901 atttgttaaa caagaaaaga aatcctaata cgactttgtc aagtcgcacg tatttcggtt
      961 ttaacctgtc ccgacggctg aagcgggcag aatacggatg cagtgctgta tcccacactg
     1021 tttacggaat agaatcaatt ctctgcatcc tgccagccga tatcgtcttc ttt
//
LOCUS       DAOQGJ010001866         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119902_length_1073_cov_2.820236, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001866 DAOQGJ010000000
VERSION     DAOQGJ010001866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119902_length_1073_cov_2.820236"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PK683_22095"
     CDS             <1..549
                     /locus_tag="PK683_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020763978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNH11206.1"
                     /translation="ARKRRKKVTSIDKANVLTTSVFWREVVVNLHKKEYSDVELNHLY
                     VDNAAMQLIVRPTQFDVVLCENMFGDILSDEASVITGSIGMLPSASLSESGFGLYEPS
                     GGSAPDIAGKGIANPIAQILSAALLLRYSFSMETEALRIEKAVREVIKKGLRTGDIAE
                     KDSQVVGTEEIGSEIEKAIERV"
     gene            553..975
                     /locus_tag="PK683_22100"
     CDS             553..975
                     /locus_tag="PK683_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH11207.1"
                     /translation="MSQGQGISASGRPYTILVAEPSKFQAKQLQQILESEGYKIIAIA
                     EDGKELMKLYQENRQVDLIIMEVCMPVIDGYAAFWDLRDASPILPKIFFISEENSPGV
                     VKSLLDNGAIDYYVKPIKREKILEKVKLAIEKVNPTFK"
BASE COUNT          349 a          209 c          231 g          284 t
ORIGIN      
        1 gccagaaaaa gaaggaaaaa agtcacaagc atcgacaagg caaatgtgct cactacttct
       61 gtattctgga gagaggtcgt agtcaatctc cataaaaaag aatattctga tgttgaattg
      121 aaccatcttt acgttgataa tgcagccatg cagctgattg tccgaccaac ccagttcgac
      181 gtcgttctct gcgaaaatat gttcggtgac attctttccg atgaagcctc agtgattaca
      241 ggatcaattg gaatgcttcc ttcagcttcc ctgtctgaat ccggattcgg tctgtacgag
      301 ccttccgggg gatctgcacc tgatattgcc ggaaaaggta ttgcaaatcc gattgcacag
      361 attctgtctg ctgctcttct actgagatat tcgttttcca tggaaacaga agcgcttaga
      421 attgaaaaag ctgtacggga agtaataaaa aaaggtttgc gtacagggga cattgcagaa
      481 aaagattctc aagtagtggg aaccgaagag attggttctg aaatagaaaa agccattgag
      541 agggtctaat cgatgagtca aggtcaggga atttccgcat ccggcagacc atatactatt
      601 ttagtagccg aaccttccaa atttcaagca aagcagctcc agcagattct ggaatcggaa
      661 ggctataaaa tcatagctat tgccgaagac ggtaaagaac tgatgaaact ttaccaggaa
      721 aacaggcagg tggatctgat tattatggaa gtctgtatgc ctgtgattga cggttatgct
      781 gccttctggg atcttagaga cgccagccca attcttccca aaatcttctt catttctgaa
      841 gaaaactctc ccggagtggt aaaaagcctg ctcgacaacg gtgcaattga ctattatgtc
      901 aagccgatca aaagagaaaa gatattggaa aaagtaaagc tggcaattga aaaagtaaac
      961 cctactttca aatgatcctc aaaaatggat agtcatatac accttagctc aaaaactgga
     1021 tcttaaatga aatttctttt aactatcatt ttaaaaattt aagatggaaa att
//
LOCUS       DAOQGJ010001867         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_119935_length_1073_cov_2.349705, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001867 DAOQGJ010000000
VERSION     DAOQGJ010001867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119935_length_1073_cov_2.349705"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PK683_22105"
     CDS             complement(<1..484)
                     /locus_tag="PK683_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11208.1"
                     /translation="MKKILFQDTTFRDGFQSFFGARVLTDDFLEGVRSSVKAGITHFE
                     AGGGARFQTLFLYCGESAFDMMDRFREAAGPEANLQTLARGISVVALEAQPKDMIDLH
                     AKLFKKHGMTHIRNFDALNDARNLLFSGQAIKNAGLHHQVVITLMELPPGASGAHDPE
                     F"
     gene            complement(500..760)
                     /locus_tag="PK683_22110"
     CDS             complement(500..760)
                     /locus_tag="PK683_22110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HNH11209.1"
                     /translation="MLDLLIEGCKLMVLGMGFVYIYLYLMIKLIHIVSRLFEKVTQKE
                     YEETILKAESERRNKAAQKNVDESEIIAVISAAVAAHKASKA"
BASE COUNT          311 a          214 c          193 g          355 t
ORIGIN      
        1 aaaattccgg atcatgagcc ccagatgcac ccggtgggag ctccatcaga gttataacga
       61 cctgatgatg cagtcctgca tttttgatag cctgtcctga aaaaagcaga tttctcgcat
      121 catttaaagc atcgaaattt cggatatgag tcattccatg ctttttaaaa agtttagcat
      181 gaaggtctat catatctttt ggctgggcct caagtgcaac aacactgatt cctctcgcca
      241 aggtctgcag attggcttca ggacctgcgg cttctctgaa tctgtccatc atatcaaaag
      301 cagattctcc gcagtataaa aacagagtct gaaacctggc tccgcctcca gcttcgaaat
      361 gggtgattcc agcctttact gaactccgaa ctccttctaa aaaatcatcc gtaagaactc
      421 tggcaccgaa aaaagactga aagccgtcac ggaaagtagt atcctgaaat agaatttttt
      481 tcatttgaat ctccctgatt tatgccttgg aggctttatg tgctgcaaca gctgcagaaa
      541 tcactgctat aatttcgctt tcgtccacat ttttttgagc cgctttgttt cggcgttcgg
      601 attcagcttt caatattgtt tcttcatatt ctttttgcgt cactttctca aaaagacgtg
      661 aaacaatatg aatcagcttt atcatcaggt acagatagat atatacaaat cccataccca
      721 ggaccattaa cttacaacct tctatgagca gatctaacat tatgtgaaca atgacagtaa
      781 aggtatttga atcaataggt ttttacataa tctttaattt atttttcaat cctgcggata
      841 tgcggttttt tctgttcatc tgatctattg catttttatg cttcaaatga tatgtttttg
      901 aaaatgagtt taaggctcat aattattgct tcattttcag ctttgcaaaa agggtcgttt
      961 tgacggtatt taaggtattc cttaaatcgg tgaggaattt gtattattga aatataaaat
     1021 atctcaaaat aattatgaat attatcttgg atttacagag cgaagaggat gct
//
LOCUS       DAOQGJ010001868         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120082_length_1072_cov_2.858407, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001868 DAOQGJ010000000
VERSION     DAOQGJ010001868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120082_length_1072_cov_2.858407"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PK683_22115"
     CDS             complement(<1..704)
                     /locus_tag="PK683_22115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11210.1"
                     /translation="MYSLPTVKASGGEDKIIDWEKIAKEENSSDGPGFFYHDCYQGVE
                     VKSVSSNLKPRGKKNYSAQNLTDPDPMTAWMCSNNGGIGESFEIISPVVNLIYNGYQA
                     SPKIWKENSRVKKFKVYKNNRPLCYLILKDQMGEQRFELPDSEISDHYAKNVYKFEIL
                     EVYRGSKWKDVGISHIDFAGCCFSEDAVLSSPSGNIKIKDAHKISELITINPDTLEIT
                     SEPVKKIVSQKHITLL"
BASE COUNT          305 a          201 c          197 g          369 t
ORIGIN      
        1 tgcagtaaag ttatatgctt ttgtgatact atttttttga caggttcact tgttatttcc
       61 agagtatccg gattaattgt aatcaattct gaaattttat gagcatcctt aattttaata
      121 tttccggatg gactggacag tacagcatct tcggaaaagc agcagccggc aaaatcgata
      181 tgagagattc cgacatcctt ccattttgaa cctctgtaaa cttccaggat ttcaaattta
      241 tagacattct ttgcatagtg atctgatatt tctgaatcgg gcagttcaaa tctttgttct
      301 cccatttgat cttttaaaat caaataacaa agaggtctat tgtttttgta taccttaaat
      361 ttcttgactc gagaattctc tttccaaatt tttggacttg cctgataccc attgtagatc
      421 agattcacaa caggtgaaat gatttcaaaa gattctccta ttccgccatt atttgagcac
      481 atccatgcag tcatagggtc ggggtctgtg agattctggg cggaataatt tttctttcct
      541 cgaggtttca ggttggaaga aacagactta acttctactc cctgatagca gtcatgataa
      601 aaaaatcccg gtccgtccga tgagttttct tctttggcaa ttttctccca gtcgataatt
      661 ttgtcttccc cgcccgatgc tttgacggtg ggcagtgaat acaaagctgt ctgtaaagaa
      721 aagattataa atagcagttt caaaattttc ataatggtca gcctctgctc acttgaattt
      781 gagtaccaga atctatacat ctttttgcct cttgaatccc ttcctgcagg tcccatactg
      841 aagtctttaa tggctctgtt ccaaaaagtc aactattata atatattggt tttagatttt
      901 ctctgccaaa tcaatttttt ctgtcacctg tccgtttttt acaatacaga aacagatttg
      961 aatgataaga ataatcattc tggagaatag gagagtaaaa tgaaactgag aagggaattg
     1021 cttccgattt ttatgatgca gactgatgca gagaatgggg atccggagaa gt
//
LOCUS       DAOQGJ010001869         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120269_length_1071_cov_3.305118, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001869 DAOQGJ010000000
VERSION     DAOQGJ010001869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120269_length_1071_cov_3.305118"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1071)
                     /locus_tag="PK683_22120"
     CDS             complement(<1..>1071)
                     /locus_tag="PK683_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNH11211.1"
                     /translation="VFLISMNNVTSFVMAFSTTVISSVYGNRIRKRLDFLKIGFLIAG
                     IQMLVATSGYLLDSRPFWNSLSNQNFLKIFLSSNILKVYLACLLNGFACATAAQFLLP
                     ILEYLFNIPTRFKLLELADTGHPLLQALLTKAPSTYTHTFLVAAMSERAAQNLGLDWL
                     LVRVGVYFHDIGKIPNAGFFVENQHLIPKKEHIDRNNPGLAAKIVIDHVLDGIEMAKK
                     ARLPREVISFIPEHHGTSTMAFFYHKALAELSPNQRKKIRKKDFQYPGPKPQRKETAI
                     VMIADSVEAASRSLDTVSAESLEELIQKIIGTKLAENQLDECGLTLGDLNIIKNSFKE
                     ILLSSLHQRPKYPNTEETKKLE"
BASE COUNT          294 a          229 c          241 g          307 t
ORIGIN      
        1 ttttcgagtt tttttgtttc ttccgtattc ggatacttcg gtctctgatg cagactcgaa
       61 agaaggattt ccttgaatga atttttaatg atattcagat cacccagagt cagtccgcat
      121 tcatccagct ggttctctgc aagttttgta cctataatct tctgtatcaa ctcttcgaga
      181 gattccgcac tgactgtgtc cagcgagcgg ctcgccgcct ccacagaatc agcgatcatt
      241 acaattgcag tttcctttct ctgcggcttg ggcccgggat actgaaaatc tttctttcgg
      301 atcttctttc tctggtttgg ggaaagttct gcaagagcct tatgatagaa gaatgccatt
      361 gtgcttgttc catggtgttc aggaataaaa ctgatgactt ctctcggcag tcttgctttc
      421 tttgccatct ctatcccgtc cagaacatga tcaatgacta tcttagcagc aagaccggga
      481 ttgtttctgt caatatgttc cttcttagga atcagatgct gattttccac gaagaaacct
      541 gcattgggaa tctttccgat atcatgaaaa tatacaccaa ccctgaccag aagccagtcc
      601 agtccgagat tctgagcagc tctttcagac atcgctgcaa caaggaaagt atgagtgtaa
      661 gtggaaggcg cctttgtcag aagagcctga agaagaggat ggcctgtatc tgccagttcc
      721 agaagcttga atcttgtggg aatgttgaag agatactcaa gtatcggaag aagaaactgt
      781 gctgcggttg cacaggcaaa cccgttcaga agacaggcaa gatacacttt caggatattg
      841 gaagaaagaa aaattttgag aaagttctga ttggaaagac tgttccagaa aggtcttgag
      901 tcaagcagat agccgctcgt agctacaagc atctgaatac ctgcaatcag gaagccgatt
      961 ttcagaaaat caagcctttt cctgatcctg tttccgtaaa cggatgaaat cacagttgtg
     1021 gaaaatgcca tgacgaagga tgtaacattg ttcatggaaa tcaggaagac t
//
LOCUS       DAOQGJ010001870         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120477_length_1070_cov_4.402956, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001870 DAOQGJ010000000
VERSION     DAOQGJ010001870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120477_length_1070_cov_4.402956"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..838
                     /locus_tag="PK683_22125"
     CDS             197..838
                     /locus_tag="PK683_22125"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNH11212.1"
                     /translation="MKIDEKFNSISHFLGAVFSLIGGIFLIKYAYSTGDTWKISGSII
                     YIITLLTVLSASTVYHGVSGSKKKIFQILDRISIFYLIAGTYTPFLFVIDHPLRWAVL
                     GWVWGLAVFGTVFILVFKEKYDLLATLLYLFAGWTIMVDIKGFYTIFPMEGFYWVVSG
                     GILYTIGAVFYSLDNKLKYNHEIWHIFVLLASFCFFISVYNYVIPYTALTAVK"
BASE COUNT          306 a          201 c          197 g          366 t
ORIGIN      
        1 taacagcaat gttacttacg ttcagtacac tggacacact agagctcctt ccgttacttc
       61 cactggattg tacgttgacc atggtgaaat caaaagcgaa ggctggagat aatcctctgt
      121 aaaagttttt ctgagacggg ttcgcccgtc tcgagatttt tcgcaacttt tcacgcaaac
      181 tttcatctta ttttatatga aaatagatga aaagttcaat tccatatctc atttccttgg
      241 tgctgtattc agcttaattg gaggaatatt cctgataaaa tatgcttact ctacaggaga
      301 tacttggaaa atttcaggaa gtattattta tattatcact ctgctgactg tattgtccgc
      361 ctcaacagtt taccatgggg tttccggaag taaaaaaaag atatttcaga tattggacag
      421 gatttccatt ttctatctta tcgcaggcac atatacacct tttctttttg taatagacca
      481 tcctctcaga tgggcagttc ttggatgggt ctggggactt gctgtcttcg gaactgtttt
      541 cattcttgta ttcaaagaaa agtatgatct tctcgctaca cttctctacc tgtttgccgg
      601 ctggacaatc atggtggaca tcaaagggtt ttatacaatc ttcccgatgg aagggtttta
      661 ctgggttgta tccggaggca ttctttatac aataggagct gtgttttatt cacttgacaa
      721 caaacttaag tacaatcatg aaatctggca tatatttgta cttcttgcaa gtttctgttt
      781 ttttatatcc gtttataatt atgtaattcc atatactgct ttgactgcgg taaagtaaat
      841 acattcagca gataagtatg tatacaggca gcttcaataa tgacattgcg gctgtattac
      901 ctatttgcaa gaaatttctt ttaaagggca gaaaatgtcc ctttccggtc cagacgaaga
      961 cccttaaatc tatactagtt ttcaaaagtc tttgcgtatt tattcacgac tcagtaaaat
     1021 caagtctgtc agccggaaaa agcgcaatta tttatgaaaa tgagtataaa
//
LOCUS       DAOQGJ010001871         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120484_length_1070_cov_3.959606, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001871 DAOQGJ010000000
VERSION     DAOQGJ010001871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120484_length_1070_cov_3.959606"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..636)
                     /locus_tag="PK683_22130"
     CDS             complement(163..636)
                     /locus_tag="PK683_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11213.1"
                     /translation="MKYTFTIIIALTAIFFSDCTNRKPNPNTGLKTLVLNNFLNGTAS
                     DKNKSEVILTRIDTVSYTGQCFDTFTVNGITVSPSNYYNTFPGGSGGAFNSNLKRWAL
                     STSDCTALGFTGGTNTNFGSSTQRPDPNSGFTFKMYQCDPGNNPCSKSAITAAGF"
     gene            complement(647..>1070)
                     /locus_tag="PK683_22135"
     CDS             complement(647..>1070)
                     /locus_tag="PK683_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11214.1"
                     /translation="TWDGTFTWKGIYLSGAYSKFRGAASNNTWGWHTTVGYNIPVGKY
                     YIMPVLKYEHLQGDWNRNGKIDPTDSLTVYWAGLNLFGDKHHFKAQLYYQVLGNKFTV
                     DPNTGNAMAIDDRRVYLQFQANFFAGTVSPEAYSFRQN"
BASE COUNT          302 a          225 c          217 g          326 t
ORIGIN      
        1 atgaattgtc cattgactgc ataatcccca aagattccgc cagtttcaga aaattggcga
       61 aggaacttaa gcacagtccc ttcgcggctg tttgtgaaac cagcataaac tttaaagctg
      121 atgcggacag gtacagaaat tcagctttta tgcaccccca acttaaaacc ctgcagcagt
      181 tattgccgac ttggagcagg gattgtttcc cggatcacac tgatacatct taaaagtaaa
      241 tccgctgttc ggatcaggtc tttgtgtgga tgatccgaag tttgtattcg ttcctccagt
      301 aaatcccaaa gctgtacagt cagaagtgga cagagcccat cttttaagat tggaattgaa
      361 tgccccgcct gagcctcccg gaaacgtgtt gtagtaattg gacggtgaaa cagtaattcc
      421 gtttacagta aatgtatcga aacactgacc tgtatatgaa acagtatcaa tacgagtcag
      481 tataacttca gatttgtttt tatctgaagc tgttccgttc aggaaattgt tcagcacaag
      541 agttttcagt cctgtatttg gattcggttt tctgttagta cagtccgaaa agaaaattgc
      601 agtaagtgca atgattatag taaaggtata tttcatttca tcctccttag ttctgtctga
      661 aactgtatgc ttcaggtgat acagttcctg caaagaaatt tgcctgaaac tgcagataaa
      721 ctcttctgtc gtcaatagcc atagcatttc cggtatttgg gtccactgta aatttgtttc
      781 caagaacctg atagtaaagc tgcgctttaa aatgatgctt gtctccaaaa aggtttaagc
      841 ctgcccaata aactgtaaga gaatctgtgg gatcaatttt cccatttctg ttccagtctc
      901 cctgcagatg ttcatacttc agtacaggca taatatagta ttttcctaca ggaatattgt
      961 atcctactgt agtatgccag ccccatgtat tgttagatgc tgcgcctctg aattttgagt
     1021 aagcgcctga cagataaatg cccttccagg tgaatgtgcc atcccaagta
//
LOCUS       DAOQGJ010001872         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120521_length_1070_cov_3.025616, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001872 DAOQGJ010000000
VERSION     DAOQGJ010001872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120521_length_1070_cov_3.025616"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..998)
                     /locus_tag="PK683_22140"
     CDS             complement(138..998)
                     /locus_tag="PK683_22140"
                     /inference="COORDINATES: protein motif:HMM:NF033170.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13355 family lipoprotein"
                     /protein_id="HNH11215.1"
                     /translation="MSVFPILQKISILILSLVFFIHCASSSSKSGKTKAAAMMLLLSG
                     SVPKSSAAPIVQPDGLADSVTSAPNHTGEGFKNKSLAVNGVRGEGQFAGSTDVFSLAQ
                     TGAGASIVLEWQGKRILNGAGIDFNVFENPFQNSDTPSLLFMEPFFVEVSADNINYCG
                     FNPLYTYSTPSQYSRNPAHWSRFAGITPVKYNVEKNPLTGNDIFDVSKAGGDAFDLDS
                     LSADNTYSIGCSETLKNTLKTSGFVYLRLTAASARINPATGAYYPVDSGAYDNGPDID
                     GVIARYTASR"
BASE COUNT          273 a          242 c          248 g          307 t
ORIGIN      
        1 cacgccgttg ccggcgcggc tgcgcgcggc ctggcagaaa tgaccctgtg ccagaaattg
       61 aacctcagtt gaatcggaaa tctgcagatc agaaattcct aaactgctga tgatttctgc
      121 tgctttggct gaattcatta tctcgatgct gtatatcttg caatgactcc gtctatatcc
      181 ggtccgttat cgtatgcacc tgagtctacc ggataatagg cgcctgttgc ggggttgatt
      241 ctggcactag cagctgtcag cctcagatac acaaagccgg atgttttcag cgtgttctta
      301 agagtttcac tgcacccgat gctgtaagta ttgtctgcac ttaaactgtc cagatcaaat
      361 gcatctcctc ctgccttgga aacatcaaat atatcgtttc ctgtcagagg atttttttcc
      421 acgttgtatt ttaccggagt gatccccgca aatctgctcc agtgagcagg gtttctggaa
      481 tactgagacg gtgtactgta tgtatataaa ggattaaatc cgcaataatt tatattgtct
      541 gcactgactt ccacaaaaaa tggttccata aacagcaggg aaggagtgtc tgaattctgg
      601 aacggatttt caaatacgtt aaaatcaatt cctgctccgt tcagtatcct ttttccctgc
      661 cactctagaa ctatggaagc tcctgctccg gtctgggcca ggctgaatac gtccgtggaa
      721 cctgcaaact gtccttctcc tcttactcca ttcactgcaa gggacttgtt tttgaatcct
      781 tcaccggtat gattcggggc agaagtgact gaatccgcaa gtccatcagg ctgaacaatc
      841 ggtgcggctg atgattttgg cacacttccg gaaagaagga gcatcatcgc cgctgcctta
      901 gttttgccgg atttggagga agaggatgcg caatgaatga aaaaaaccag agacagaatg
      961 agtatggaaa ttttttgcag gatcggaaat acgctcatat tttcatttcg aaaaatgagt
     1021 tggagaatgc aaagattttt tgcatcgttt cagggagatg aggcatctct
//
LOCUS       DAOQGJ010001873         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120559_length_1070_cov_2.523153, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001873 DAOQGJ010000000
VERSION     DAOQGJ010001873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120559_length_1070_cov_2.523153"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..546
                     /locus_tag="PK683_22145"
     CDS             292..546
                     /locus_tag="PK683_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11216.1"
                     /translation="MKYVYLSPVITIISLIDKVFLTFPVYYVHDIVKIFIIPIGFIYY
                     LEETVRDTKTWLAEDPDSDESEKHIDLNEEYEKFLRRKNK"
     gene            734..991
                     /locus_tag="PK683_22150"
     CDS             734..991
                     /locus_tag="PK683_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11217.1"
                     /translation="MEKTLKELLSLWMIGEDMKKYMFFSLNIFWWNRIAKIMQFIAAF
                     TVIADILGAARLNHIGMESYRLRLRSHWEEELLMRESMIFL"
BASE COUNT          331 a          176 c          238 g          325 t
ORIGIN      
        1 cctctttcag gtttgtcttc tctgcctcac tgtatatcgg catgaatgac tgtgaatctt
       61 catttcagga cacctgagat tttatggaat ccgcagggtg acctgtttta tcctgcgtct
      121 tttgacaact gtgaaacgat cagacgagga agagttccat ccagattcct ttccgataat
      181 tcacttagga aaaaaaatag tccgtctttt tttggaggag agcaaggcat tattgatact
      241 ctcattccgt ataaagtatt gacttcttcc aatatcagga cagaagataa aatgaaatat
      301 gtttatctta gtccggttat aaccataata agccttattg ataaggtttt tttaacattt
      361 cctgtatact atgttcatga cattgtaaag atatttatca ttccgatagg ttttatttac
      421 tatctggagg aaactgtcag agatacgaag acttggcttg cagaagatcc ggattctgat
      481 gaaagcgaaa aacatataga tcttaatgaa gagtatgaga agtttctaag aagaaaaaat
      541 aagtgaggat tagtacttgg gataatgctt ttgggtcaaa gaaagcctca gcgaaaggga
      601 tagcaacatt ggaggcggtg tcaagtttct gcggatagcg aagcgtaaat gattgacggc
      661 agccgaaggc ctgttcatat tgttttgagt gggttgttgc ttttactgat ttaaaactta
      721 gaaaatctcc tgtatggaaa agacactgaa agaactgctg agtctgtgga tgatcggaga
      781 ggatatgaag aaatatatgt tcttttctct gaacattttt tggtggaaca gaattgcaaa
      841 aataatgcag tttattgcgg cattcacagt cattgcagac atactggggg cagccagact
      901 gaaccacatc ggaatggaat catacagact ccgcctgcga tctcactggg aagaagagct
      961 gttgatgaga gaatcaatga ttttcctctg atcaaggtga aatgacggtt ctggcatatt
     1021 gtcaatacgt atagaaatat tttttcacac agaaattatg gagaccacag
//
LOCUS       DAOQGJ010001874         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120625_length_1070_cov_1.922167, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001874 DAOQGJ010000000
VERSION     DAOQGJ010001874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120625_length_1070_cov_1.922167"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK683_22155"
     CDS             <1..234
                     /locus_tag="PK683_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNH11218.1"
                     /translation="TVTAFEAKTHLSELLKKVMNGDSFIVTLRGKPVAKIISFREESS
                     SEIESAVTRLSEIRKTVKGGLSIKEMIEEGRKY"
     gene            231..647
                     /locus_tag="PK683_22160"
     CDS             231..647
                     /locus_tag="PK683_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH11219.1"
                     /translation="MKTWVIDCSFTAAMFLPDEKSDLADQFFSRIGSEFKAIIPAIWW
                     YELNNVLLVSVRRKRLTNENAETVWKTFESFPIEMDKNFSFQTMKSIFELGKKHDLSV
                     YDSAYLELCIRKKAGIVSLDEKLQKSAVKEKIPVFK"
     gene            768..>1070
                     /locus_tag="PK683_22165"
     CDS             768..>1070
                     /locus_tag="PK683_22165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH11220.1"
                     /translation="MAAIVSVKAGKYIFKEGDSGTSMYIVMEGVVDIHIVINGEETNL
                     AVMKQGDFFGEMALFREKPRSASARVMQDVRLAVIESRAQLEKFLCDNPLFAAKMVN"
BASE COUNT          364 a          192 c          235 g          279 t
ORIGIN      
        1 actgtaactg catttgaggc caaaactcat ctctctgaac ttttaaaaaa agtaatgaac
       61 ggtgattcat ttatagtcac actgagagga aaacctgtgg caaaaatcat ttcctttcgg
      121 gaagaaagca gttcggaaat tgaatctgcc gttacaagat taagcgagat cagaaaaaca
      181 gtgaagggcg gcctcagcat aaaagaaatg atcgaagaag gcagaaagta ttgaagactt
      241 gggtgattga ctgttcattt actgcagcga tgttcctgcc cgacgaaaaa tcagatcttg
      301 cagatcaatt tttttccagg atcggttctg aattcaaagc aattattcct gccatctggt
      361 ggtatgaact caataatgtg cttctggttt ctgtcaggcg caaaagactg acaaatgaaa
      421 atgcagaaac agtctggaag acttttgaaa gttttcctat tgaaatggat aaaaactttt
      481 cttttcagac aatgaaaagc atctttgaac ttgggaaaaa acacgatcta tctgtttacg
      541 attctgccta tctggaactg tgcattcgaa aaaaagcagg tatcgtaagc ctagacgaaa
      601 aactgcagaa atcagctgta aaagaaaaga ttcccgtttt taaataaatt acttgtccta
      661 aatcagattc tgaagaaaat agaagaatca attgaacttt aaggaaaagt gcagtttttt
      721 caaattgttg gataaaggaa aaacgcaccg gacaaagacg ggtcagtatg gcagcaatag
      781 tcagcgtcaa agctggtaaa tatatattca aagaaggcga ttccggcact tccatgtaca
      841 ttgtaatgga aggcgtggta gatattcata tagtaatcaa cggagaagaa acaaatctgg
      901 ccgtaatgaa gcagggagat tttttcggtg agatggccct gttccgcgaa aaacccagaa
      961 gtgccagtgc cagagtgatg caggatgtcc gtcttgcagt catagaaagc cgtgcccagc
     1021 tcgaaaaatt cctctgcgac aatcctttgt ttgccgccaa gatggtcaat
//
LOCUS       DAOQGJ010001875         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120784_length_1069_cov_2.428008, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001875 DAOQGJ010000000
VERSION     DAOQGJ010001875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120784_length_1069_cov_2.428008"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK683_22170"
     CDS             complement(<1..210)
                     /locus_tag="PK683_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNH11221.1"
                     /translation="MKHFKYTSATLLILLISCQTLLRPRKDSNASLSLKKPDYSKLEL
                     WAAHPDKKDSADEVPEESGLKDNQSN"
     gene            416..634
                     /locus_tag="PK683_22175"
     CDS             416..634
                     /locus_tag="PK683_22175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11222.1"
                     /translation="MFLKILFVFGMIFSSVYSQTDSSASEKNIISGSSERKKAAEDKS
                     KSPKQPKGMPELKTQETHSKESKSKKSE"
     gene            639..>1069
                     /locus_tag="PK683_22180"
     CDS             639..>1069
                     /locus_tag="PK683_22180"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH11223.1"
                     /translation="MDYIESYGVQIPFYVNGEGFPLFIFNGFTCSQPNLKYLIADLSK
                     KYKVISWDYAGHGKSGTPSDFSGISVDSFAEDAARIMDRLKVEKAVFLGYSLGAQVML
                     QNYLENRGRAEVLIRLCGFPGNAFDSFLRSPLSANVVELLN"
BASE COUNT          291 a          215 c          245 g          318 t
ORIGIN      
        1 attggactga ttgtccttca gaccggattc ttcaggcacc tcgtctgcac tgtctttttt
       61 atccggatgg gccgcccaaa gttccaattt ggaataatcc ggctttttca gggacagact
      121 tgcatttgaa tcctttctcg gacgaagaag agtctggcag gaaataagta agataagcag
      181 ggttgcagat gtatatttga aatgcttcat aggaatacta tggactttcc gtgatttcgg
      241 tcaagccttg gaagtcccga atggatttat tttcgggccg ggataaggga tcagtttttg
      301 attgaaccgg ggagccgccc ttcgttgatt taaagcttct ttttctcttg actctaattt
      361 ttcggaaagc cttatgtaaa tattatcctc atttgaacgg ggctacggag gcatcatgtt
      421 tcttaaaatc ctatttgttt tcggtatgat tttttcctca gtctattctc agactgattc
      481 ttctgcttca gaaaaaaata tcatcagcgg gagttctgaa agaaagaaag ctgctgaaga
      541 caagagcaaa tctcccaaac agcctaaggg aatgcctgaa cttaagactc aggaaactca
      601 ttccaaagaa tctaaatcaa aaaaatcgga ataagaaaat ggattatatc gaaagttacg
      661 gagtgcagat tcctttctat gtgaacggag aagggtttcc tcttttcatt ttcaacggtt
      721 ttacctgcag tcagcctaat cttaagtacc tgattgcaga cctttccaaa aaatacaaag
      781 tgatttcctg ggattacgca ggtcacggca agtcaggaac tccgtccgat ttttccggaa
      841 tctctgtgga ttcctttgcc gaggatgcag caaggattat ggacaggctg aaagtggaga
      901 aggcagtgtt tctcggttac agcctgggag cacaggtaat gcttcagaat tatctggaaa
      961 acagaggaag agccgaggtt ctgatcaggc tctgcggttt tcccggaaat gcatttgatt
     1021 catttcttcg ctctccgctt tccgcaaatg tagtggaact gctgaacag
//
LOCUS       DAOQGJ010001876         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120927_length_1068_cov_3.666338, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001876 DAOQGJ010000000
VERSION     DAOQGJ010001876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120927_length_1068_cov_3.666338"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..639)
                     /locus_tag="PK683_22185"
     CDS             complement(322..639)
                     /locus_tag="PK683_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11224.1"
                     /translation="MKNFIIIMILSLHFSECQVPAGSFRMMSASLPAESETIGSSRVY
                     GEHCRFYNSITGYAFPQMDLAVKDALSKVPGAKGLKDASIAEKYSPVFSYYCYEVDGL
                     PVK"
     gene            complement(639..926)
                     /locus_tag="PK683_22190"
     CDS             complement(639..926)
                     /locus_tag="PK683_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11225.1"
                     /translation="MDFRQKLFSFTVLIFTGGCVTWELGKVGYIEPSKLEIEPAEKKL
                     ILSECSFLSGGPFLERAVEKAEPGRLKNAVIIKELRGTTSCYRLYGEKESQ"
BASE COUNT          316 a          245 c          191 g          316 t
ORIGIN      
        1 actgtcagaa aaacacgggc aaacttagag gaaatctttt cataaaaatc tggcatagcg
       61 gtcattttat tatccgattt tactgaagtc aatgaaatca gtttctcatt ttaatattcg
      121 cagaaaacca ctattccatt ttgagcaaaa aaacacgaaa attgaaatat ttactttgcg
      181 aaacattaga gaaatgtaat ggatcagcaa aatcagccgc ttttgagaat tcgaccccac
      241 aagccggcct cagatctctg caaatttaac tttctgggaa tgggtgtaaa gacctactgc
      301 tgggataatt tctgactgaa cctactttac cgggagaccg tcaacttcat agcaataata
      361 agagaaaacg gggctgtatt tttccgcgat acttgcatct ttcaatcctt ttgctccggg
      421 aaccttgctc aatgcatctt ttacggcaag atccatttgc ggaaacgcat atccggtaat
      481 gctgttgtaa aaccggcaat gttcaccgta gaccctggaa cttcctattg tttctgattc
      541 agccggaaga gaggcgctca tcattctgaa tgatccggca gggacctggc attcggaaaa
      601 atgcaatgat aaaatcatta taataataaa atttttcatt attgactttc cttttcaccg
      661 taaagtctgt aacaggatgt tgtccctctc aattctttta tgatgaccgc atttttcaat
      721 ctgcccggtt ccgctttctc aacagctctt tccagaaaag gtcctccgct cagaaaggag
      781 cattcagaga gaatcagttt tttttcagca ggctctattt caagttttga cggctctatg
      841 tagcccacct tcccaagctc ccaagtaacg catccgccag taaatataag aactgtaaaa
      901 gaaaataatt tctgcctgaa atccataacc ctgaactcct taaaaaggtt tttattctgc
      961 cttctttttc aactgattaa ctcaccttgc gcctacggct gaccgctctc gccaacattt
     1021 agaagctggc tgccaacatt tagaagctgg ctgccaacat ttagaagc
//
LOCUS       DAOQGJ010001877         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_120984_length_1068_cov_2.690030, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001877 DAOQGJ010000000
VERSION     DAOQGJ010001877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120984_length_1068_cov_2.690030"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PK683_22195"
     CDS             <1..726
                     /locus_tag="PK683_22195"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNH11226.1"
                     /translation="SRDELQIYSLIRNRFLESFLPPAVYREYKWTAESKGFALTASKE
                     FCTDPGYLAFRREKTAEKYHDFQMKKNDILISKIMNMKKDKTEPPPRFTEASLVDRLE
                     KTGIGRPSTYSSVIETLLKRKYAERREKSLHPTDLGERVIKRMEEEFSSIIEDGYTAQ
                     LEKELDEIESGQKKKLVFLSEFYSKFLEMLGNEKKLKKNIKKEKTICPLCGQGHLIRK
                     ITKKDKSYHMCSRYPDCEYLEYL"
     gene            803..>1068
                     /locus_tag="PK683_22200"
     CDS             803..>1068
                     /locus_tag="PK683_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11227.1"
                     /translation="MELKKILLEEIKEEHRNTRKILESIPVSQGSWKPTPKSMSLIHL
                     AAHMADLFSYLEIILSTKTFDLAESRTPIDSIREKQVILSELDA"
BASE COUNT          385 a          205 c          227 g          251 t
ORIGIN      
        1 tccagggatg agctgcagat ctattcactg atcagaaaca gatttctgga aagcttcctt
       61 cctccggccg tttacaggga atataagtgg accgcagaat caaaaggatt tgccctgacc
      121 gcttcaaagg aattctgcac tgatccgggc tatctggctt tcagaaggga aaaaactgca
      181 gagaaatatc atgattttca gatgaaaaaa aatgatattc tcatttcgaa gattatgaat
      241 atgaagaagg acaaaacgga gcctcctccc aggtttacag aagcctcact ggtggacaga
      301 ctggagaaaa caggaatcgg aagaccttcg acctattcat cagtcatcga aacgcttctg
      361 aaaagaaagt atgctgaaag aagagaaaaa tcccttcatc cgactgatct tggtgagagg
      421 gtaattaaaa ggatggaaga agagttttca tccattattg aagacgggta cacagcgcag
      481 ctggaaaaag aactggatga aattgaatcg ggacagaaga agaaacttgt attcctttct
      541 gaattttaca gcaaatttct ggaaatgctg ggcaatgaaa aaaaacttaa aaaaaatata
      601 aaaaaggaga aaaccatctg tcctctctgt ggacagggac atctcatcag aaaaatcacg
      661 aagaaggaca aaagttatca tatgtgcagc aggtacccgg actgcgaata tctggaatat
      721 ctctaggaag tcggtaaaaa actccgcaaa attcacttga attaataaaa ctcttcctgt
      781 ctgctgattg cagaggaata aaatggaact gaaaaaaatt ctactggaag agattaagga
      841 agaacacaga aacacaagaa aaatcctgga atcaataccc gtatcgcagg gttcatggaa
      901 accgacccca aaatcaatgt ccctgatcca tcttgctgct catatggctg atttattttc
      961 ttatttggaa atcatactga gcactaagac ctttgatctg gcagaaagca gaaccccgat
     1021 agattcaatc agagaaaaac aggtgatcct ttctgaactc gatgcctg
//
LOCUS       DAOQGJ010001878         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_121226_length_1067_cov_2.503953, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001878 DAOQGJ010000000
VERSION     DAOQGJ010001878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121226_length_1067_cov_2.503953"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PK683_22205"
     CDS             <1..923
                     /locus_tag="PK683_22205"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNH11228.1"
                     /translation="YDAGIYSIRELFGHPMMKKMDLLDIPHFNIAVPYLKKSIVTVHD
                     LTPYIMKEFFPSRLKRSYLNLIFRLIKKSASVIAVSENTRQDLIREFKYNPNAVRVNY
                     NAVDADVFRVHTKEETEFFRKKYSLPDKFYLTVGIGKEHKNFKFLLSCLLELWKEKRE
                     APPLVLAGTGGKLPDYLQELALSADDGKLILFPRIKYEELPFLYSAADLMIFPSLYEG
                     FGFPAVEAQASGCTVLSSNASVMPEILSDSAVYFDPKDRSSFLHSLNEIENNPEQKKI
                     LKERGFENITRFDWKKSAREILSLYKEIKL"
BASE COUNT          321 a          215 c          218 g          313 t
ORIGIN      
        1 cttatgatgc agggatctac agcatcaggg aactttttgg acacccgatg atgaaaaaga
       61 tggatctgct cgatatccct catttcaata ttgctgttcc atatttaaaa aagtcaatcg
      121 tcactgtgca tgatctgaca ccttacataa tgaaagaatt ttttccatcc agacttaaaa
      181 gatcatattt gaacctgatt ttccgtctga ttaaaaaatc ggcttctgtt attgcagttt
      241 ctgaaaatac caggcaggat ctgatcaggg aatttaaata caatcccaat gctgtcagag
      301 tcaattacaa tgcagttgat gcagatgtct tcagagttca tacgaaagaa gaaacggaat
      361 ttttcagaaa aaaatattct cttcccgata aattttatct cactgtaggg attggcaaag
      421 agcacaaaaa tttcaaattt cttctcagct gccttttgga attatggaag gaaaaaaggg
      481 aagcaccgcc cctcgtactc gctggaacag gaggaaaact tcctgattac cttcaggagc
      541 tggccctctc tgctgacgac ggcaaactga ttctatttcc aagaattaag tatgaagaac
      601 ttccttttct gtattctgca gcagatctga tgatattccc ctcactgtat gaaggattcg
      661 ggtttccggc agtggaagct caggcttcgg ggtgcactgt tctcagttca aatgccagcg
      721 ttatgccgga gatactgtca gattctgctg tctatttcga tcctaaagac agatcgagtt
      781 tcctgcattc actgaatgag atagagaata atcctgaaca gaaaaaaata ttgaaagaga
      841 gaggatttga gaatataaca agattcgact ggaaaaaatc cgccagggaa attctcagtc
      901 tgtataagga aattaagctt tgatttcccc ctgcagtacg cagtttagtt tttttagact
      961 ctgattggtc ccgacattca gactggtgca gaagaaaaac tgagagcggc tttgcagtcc
     1021 gctctgaatt taactcaggt agatacttct tcttccttct tcggtgc
//
LOCUS       DAOQGJ010001879         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_121372_length_1066_cov_3.679525, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001879 DAOQGJ010000000
VERSION     DAOQGJ010001879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121372_length_1066_cov_3.679525"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..967)
                     /locus_tag="PK683_22210"
     CDS             complement(128..967)
                     /locus_tag="PK683_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH11229.1"
                     /translation="MNLPLKGCTYLITGIIDSGSLALTAAKQIVAEGGEVICTGLGKT
                     PFHQNLSEKAQGYLDSTYSAFQETVRKELGTDVLTFPLDVTIDESINSFSKLLAEKNI
                     SLSGFLHSIAMDKTIKAGVVVKSILEVTKEEFFSAMDVSAYSLVSLTRSLLNYKVLKN
                     NASILALSYLGAEKVVYHPYRNIGVAKAALERIVIEMAYELGRSHQIKVNCIRFSPYT
                     GSKAGSAIQGLQEAVNFCNENSPLGNALPEDLAYESAYLLRPYGRITGEIRYVDGGYH
                     IRG"
BASE COUNT          281 a          245 c          230 g          310 t
ORIGIN      
        1 gcggtgattt agtgttcggc aggacatcat ggtttactgt atactttcat agtcttgatc
       61 ctatgcagaa tcagaagttt ggaaagtttt ccaaaaaaca ataaacagtt tctgctcagg
      121 gattacctca gccccggata tgatatcctc cgtccacata tcgaatctct cctgtgattc
      181 ttccataagg ccgaagcaga taggcagact cgtacgccag atcctccgga agagcatttc
      241 cgagaggact gttctcattg cagaaattca cagcttcctg gagtccctga attgcactgc
      301 ctgccttgct tcctgtgtat ggggagaaac ggatgcagtt gaccttgatc tgatgagatc
      361 tgcccagctc ataggccatt tcaatcacaa tacgttccag tgcagccttc gcgactccta
      421 tgtttctgta aggatggtag accacttttt ctgctcccag gtagctcagt gcaagaatgg
      481 atgcattatt tttcaatact ttgtaattta aaagagatct ggtcagagaa accaaggaat
      541 aggcggatac atccattgca ctgaagaatt cttcctttgt gacttccagg atggatttga
      601 ctacaacccc ggccttgatt gtcttgtcca ttgcgatgga gtgaaggaat ccgctcaggc
      661 tgatattttt ttctgcaagc agttttgaaa aggaattaat gctctcatca atggtgacat
      721 ccagcggaaa tgtcagaacg tctgtaccaa gttctttgcg gactgtttcc tgaaatgcac
      781 tgtaggtaga atccagataa ccctgtgcct tctctgaaag attctgatga aacggagttt
      841 tccccagccc cgtgcagatg acctcaccgc cttccgccac aatctgtttt gcagcagtca
      901 gtgcgagcga gccagaatca ataatccctg taattaaata tgtacaacct tttaatggca
      961 gattcataga tacccttcct gaagttctgt ctgcagacag tatttaaaat atgagtttta
     1021 tgtaaatgag aatcagttgc atatcagagg cctgccgacc gattct
//
LOCUS       DAOQGJ010001880         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_121382_length_1066_cov_3.359050, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001880 DAOQGJ010000000
VERSION     DAOQGJ010001880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121382_length_1066_cov_3.359050"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PK683_22215"
     CDS             complement(<1..551)
                     /locus_tag="PK683_22215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11230.1"
                     /translation="MILVLSVFLLPISSPIFTENIFLLGKEFGIDVSRISLNEQENLS
                     KVFMFIDSFQTEKLYFSEKFYRKRSKFQKLFGFPFSGKKLNEWILSRIKKIKIQKIKD
                     YTALNSGTELTLSDSFLQLSVLEQSIILVHEARHSDGREFAHTECPANFAFLSQRNPE
                     AVLSELYACDDREDGAYGFSAAF"
     gene            complement(608..>1066)
                     /locus_tag="PK683_22220"
     CDS             complement(608..>1066)
                     /locus_tag="PK683_22220"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH11231.1"
                     /translation="HGGVGYTEDFDVSRLFRDARINTIYEGTSQIQVRISSGAFLAGM
                     SEEGGFRKYMNSLKKDIIYFKEMISEQSDLMESCISAYRKITDEEKKERRAEDLINAA
                     SRHICSVLYARATGKLNEKGLAEDWKNDFRNYALDSTALMKGAEYRILRN"
BASE COUNT          316 a          233 c          188 g          329 t
ORIGIN      
        1 aaaaaagctg cagaaaatcc gtaagcaccg tcttctctgt catcgcaggc ataaagttca
       61 gaaagaactg cctcaggatt tcgctgactt agaaaggcaa aatttgcagg acattctgta
      121 tgcgcaaact ctcttccgtc tgaatgcctt gcctcatgca caagaataat gctctgttct
      181 aaaactgaaa gctgaagaaa agaatcagag agggtcagtt ctgttcctga attcaaagca
      241 gtatagtctt tgattttctg aatttttatc ttttttattc gagaaagaat ccactcattc
      301 agttttttgc cgctgaaagg aaatccgaac agtttttgaa attttgatct ttttctgtag
      361 aatttttctg aaaaataaag tttttctgtc tgaaaagaat caataaacat aaaaaccttg
      421 ctcagatttt cctgttcatt tagggaaatc ctggaaacgt caattccaaa ttctttcccc
      481 agtaagaata tgttttcagt aaaaattgga gaagaaatag gcagaagaaa aacagacagg
      541 acaagaatca tccgcaattc agccctgatc aaaaggcatc ctgtccgaca atcagaaaac
      601 aaagatttca attcctcaat atcctgtatt cagccccctt catcagcgca gtgctgtcga
      661 gagcgtaatt ccgaaaatca tttttccagt cttctgcaag tcctttttca ttcagttttc
      721 ccgtggctct tgcatagaga acagaacaga tatgtcttga tgctgcattg atcagatcct
      781 cagcccttct ttcctttttt tcctcatccg tgatcttcct gtaggcagag atgcaggatt
      841 ccatcagatc agactgttct gaaatcattt ccttgaaata tattatatct ttttttaatg
      901 agttcatata ttttcggaaa cctccctcct cagacattcc agcgagaaat gctccggaag
      961 aaattctcac ctggatctgg cttgtgccct cataaattgt gttgatcctt gcatcacgga
     1021 acagtctgga tacgtcaaaa tcctcagtgt agccgactcc gccgtg
//
LOCUS       DAOQGJ010001881         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_121433_length_1066_cov_2.613254, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001881 DAOQGJ010000000
VERSION     DAOQGJ010001881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121433_length_1066_cov_2.613254"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1066)
                     /locus_tag="PK683_22225"
     CDS             complement(<1..>1066)
                     /locus_tag="PK683_22225"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNH11232.1"
                     /translation="EKPIALGKPIDQDPSEKMSYKVYHVKGKFKAETLPVKYVLEQNS
                     LGISFRHKTLNRNNLIYVIDEIGIGKHIKKETLKNSQVLSSSYGWTIANTWAFQDTLK
                     KNSLGSPRYLSLQDTTVPYSRFNMGIRIKKNEITHRRILPGVITMYIFLFCLAGIMVV
                     GIISSLKSYKSFTNYFWTAQIILTFLMINSAEVETIEYLVDRDNIDKIKLVLNIFDLI
                     WWIVPAIVLTQAIERFLWRPLEIKTEHKIPTVVRRFLNFLIYLLTFFGIVAFVYDQKI
                     TSLLATSGVVAMIIGLAIQVNIANVFSGIAINMERPFRVGDWIKIGTMDEGRVVDITW
                     RTTRIRLRNQVMVSVPNSMAS"
BASE COUNT          295 a          227 c          215 g          329 t
ORIGIN      
        1 cagaagccat actgttagga acgctgacca tcacctgatt gcgcagacga attctggtcg
       61 ttctccaggt aatgtccacg actcttcctt catccattgt tcctatcttg atccagtctc
      121 ccacacggaa cggtcgttcc atattgatcg ctatcccgga aaatacgttg gcaatattca
      181 cctgaatagc aagacctatg atcattgcaa caacacccga ggttgcaaga agacttgtaa
      241 tcttctgatc gtatacgaat gcaacgatgc cgaagaatgt cagaagatag atgaggaaat
      301 tcagaaacct tctgactaca gtgggaatct tatgctctgt cttgatttca agaggacgcc
      361 aaagaaatct ttcaattgcc tgtgtgagca ctatggctgg cacaatccac cagataagat
      421 caaaaatatt caggacgagt tttattttgt caatattgtc tctgtccaca agatattcaa
      481 ttgtttccac ttctgcagag ttgatcatca ggaaagtaag aataatctgt gcggtccaga
      541 aatagttggt aaaggattta tatgacttaa ggcttgatat gattcccaca accatgatcc
      601 ctgcaagaca gaatagaaaa atatacattg taattacgcc aggaagtatg cgtctgtgcg
      661 tgatttcatt tttctttatc ctgattccca tattgaatct ggaatagggg actgtggtgt
      721 cctgaagact cagatacctg ggcgaaccaa gagaattttt tttcagcgtg tcctgaaatg
      781 cccaagtgtt tgctattgtc catccgtaag aggaactcag tacctgagag tttttaagag
      841 tttctttctt tatatgtttt cctattccga tttcatctat gacatagatc agattgttcc
      901 tgttcaatgt cttgtgacgg aaactgattc cgagcgaatt ctgttccaga acatatttga
      961 cgggcagagt ttctgccttg aattttccct tcacatgata gaccttatag gacattttct
     1021 ctgaaggatc ctgatctatg ggttttccca gcgcaatcgg cttctc
//
LOCUS       DAOQGJ010001882         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_121573_length_1065_cov_5.468317, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001882 DAOQGJ010000000
VERSION     DAOQGJ010001882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121573_length_1065_cov_5.468317"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PK683_22230"
     CDS             complement(<1..713)
                     /locus_tag="PK683_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11233.1"
                     /translation="MNIKTAINKTAEEIKSFRSSASESLKKFWKPEKVYILPNLFAVI
                     WAGSSLYMYILYFLSANIAVLVLSVFMTAFWIFDMLYTNTLCKHLSLHLQNVDFTADI
                     PSEIPVRFENFQEIQSVEVFSEGHTPEAKIFFFNSEKQKAADPKNLKILMKVSVRTHL
                     RLLRFRLSIRSVIGFFICWKYTDVLCDMHIWPKSVLHMESSQVRNLEDLSAKGSFLQG
                     NEDFYEYRKNPSFENPYIY"
     gene            complement(854..>1065)
                     /locus_tag="PK683_22235"
     CDS             complement(854..>1065)
                     /locus_tag="PK683_22235"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11234.1"
                     /translation="VHSLPLSPRAGISAARMAKVTAFLRNRNYVIIEDIQYVWESISA
                     HRLSFSRKEGLAASRLLLSQTEIPV"
BASE COUNT          320 a          204 c          207 g          334 t
ORIGIN      
        1 atatatatat atggattttc gaaagaagga ttttttctat attcataaaa atcctcattc
       61 ccctgcagaa aacttccttt tgcagacagg tcttccagat ttctgacttg agaagattcc
      121 atatggagaa cagattttgg ccagatatgc atatcacata aaacatcagt atatttccag
      181 cagataaaaa aaccgatgac tgaacgtatg ctcagtcgga atctgagcag tctgagatga
      241 gttcttacgg atactttcat cagaattttc agatttttcg gatctgccgc tttttgcttt
      301 tctgaattga aaaagaagat ctttgcttcg ggtgtatgcc cttctgaaaa tacttcgacc
      361 gactgaatct cctgaaagtt ttcaaatcta acaggaattt cagatggaat atccgcagtg
      421 aaatcaacat tctgcagatg caaggacaga tgtttgcaga gtgtgtttgt atacagcata
      481 tcaaatatcc agaatgcagt cataaaaacc gacagaacca gtacggcaat gtttgcggag
      541 agaaaataaa gtatgtacat atataaagaa gaacccgccc aaatgactgc aaacaggttg
      601 ggcaggatgt atactttctc cggtttccag aattttttta aagattcaga tgcagaggat
      661 ctgaaggact ttatttcttc agcagtctta ttaatagctg tttttatatt catacttatt
      721 gattatttcc aaactgaggt acaggaacca ttctgctgat tcattggagt gtttcaagtc
      781 cattgtcttt ctgccggcag ctgctgaata tctcctttca gtaatgcaat ttatgaatct
      841 tcctctcatt gaatcagacc ggaatttctg tctgagaaag cagaagtctg gatgctgcaa
      901 gtccctcttt tctggaaaaa ctgagccggt gtgcagagat ggattcccat acatattgta
      961 tatcttctat aattacataa tttctgtttc tcagaaatgc agtcaccttc gccatccttg
     1021 cagcagagat tcctgctctc ggtgaaagag gaagagagtg cacgt
//
LOCUS       DAOQGJ010001883         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_121600_length_1065_cov_3.361386, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001883 DAOQGJ010000000
VERSION     DAOQGJ010001883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121600_length_1065_cov_3.361386"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..593
                     /locus_tag="PK683_22240"
     CDS             312..593
                     /locus_tag="PK683_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11235.1"
                     /translation="MHNSNLAEEIRLLEAESVNIKKRARENYLEQVSDISEKIKQIGD
                     DAQVKAKEVLDRVGEYITENPQKSALIGIGVGFGLGVLIGLISRRRKDD"
     gene            594..956
                     /locus_tag="PK683_22245"
     CDS             594..956
                     /locus_tag="PK683_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11236.1"
                     /translation="MAKKKQPEKEKVSGRKKPKSGVVDYFAAFLEQIISYFELLFVYY
                     RKKFNENTRKSIALLIGISYFFFWILAGSGFIFHAVYELLKLQFPGNNYAPSLVLGSV
                     LLLIAGIFIRILLKRVIF"
BASE COUNT          326 a          189 c          228 g          322 t
ORIGIN      
        1 cagttccgaa aggaacagct accttgtgtc tcttcagaat ctctttagcc tgatattcgt
       61 gtactttcat tcccaatccc tgttttgtct ttcttctgag tttttttcca ctgctttacc
      121 tgtcattaaa aaaacagggg caatgaatgg ggaatttctt tggaagatgt tccctctttt
      181 gaaatgacag cttatttcac ttttctgaaa tcggagagga ggatgtactg ccgatgactt
      241 tttttcagtg cagcatcaaa aaaagcttga aaatctgaat tgtttcgcat aattatttgt
      301 gagagattac aatgcataac tcaaatttag cagaagagat cagacttttg gaagcagaat
      361 cagtgaatat caaaaaaaga gccagggaga attatttgga gcaggtatcg gacatttcag
      421 aaaaaatcaa gcagatcgga gatgatgccc aggtcaaggc aaaggaagta ttggacaggg
      481 taggagagta catcactgaa aaccctcaga aatcagctct gatcggaatc ggtgtgggat
      541 tcggactcgg tgtactgatt ggtctgatct ccagaagaag aaaagacgac tgattggcta
      601 aaaagaagca gcctgagaaa gaaaaagtct caggcagaaa aaaaccaaaa tcgggagtag
      661 tggattattt tgctgctttc cttgagcaga taatttccta ttttgaactc ctttttgtct
      721 attacagaaa aaagttcaac gaaaatacca gaaagtctat tgcactgctg atcggaatat
      781 cctatttttt cttctggata ttggccggaa gcggattcat atttcatgca gtctatgaac
      841 ttctgaaact tcagtttccg ggaaacaatt atgcaccttc tcttgtactg ggatctgtcc
      901 ttttactgat tgcgggcatt tttatcagga tactgttaaa gagagtgatt ttctgatgaa
      961 accggaggtt ccgtttaatc cgttttctga ttcggaaaat tttacgaaaa aagatccgaa
     1021 agatatgagc ctttccgaac tggagattct tctctacagt aaaag
//
LOCUS       DAOQGJ010001884         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_121865_length_1064_cov_2.486620, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001884 DAOQGJ010000000
VERSION     DAOQGJ010001884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121865_length_1064_cov_2.486620"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PK683_22250"
     CDS             complement(<1..386)
                     /locus_tag="PK683_22250"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH11237.1"
                     /translation="MKFHIITIFSILLFTQNCRWDSKEGISSNFKMMFSVLSAVQPKV
                     SSVLPKDKSTGNYRNTQIVIVLNTDMNTDTLKTALSVKSDQGAVTGDISFDGTKVVTF
                     VPKTTLNSNTTYTITVGTDAKSDRGVS"
     gene            complement(358..>1064)
                     /locus_tag="PK683_22255"
     CDS             complement(358..>1064)
                     /locus_tag="PK683_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11238.1"
                     /translation="SITKVGNKYFINGNIIDPVKDNIDFAETAVADSTAGFQSASELF
                     AKKAAGGISDYTDLVIGQKGGENKYEALVRSAVLPGWGQYNYGVKNGSSWHKNKGIAV
                     GSLFAVSLLHLAYRNGEYSKARKDFDSVHRTFLIIPFGSPAEILYFAKDKSEYSQLKK
                     ATGDVQNAALLVGLVYVLNLTDSFFSGKRFLGFGNMEKKTGFQLNAYPAMAAGTASNL
                     QKTMEMNYEISYYHNF"
BASE COUNT          281 a          248 c          211 g          324 t
ORIGIN      
        1 gaaacccctc tgtccgattt tgcatccgtt cccaccgtga tggtatacgt tgtatttgaa
       61 tttaatgttg tcttcggaac aaatgtgaca acctttgtgc cgtcaaaact gatgtcacct
      121 gtcactgctc cctgatccga cttgacggaa agtgcagtct tcagagtgtc cgtattcata
      181 tcggtgttca gaacaatgac aatctgagtg ttcctgtaat ttcctgtgct tttgtccttc
      241 ggaagaacag aggatacttt cggctgaact gcagaaagga cgctgaacat catcttgaaa
      301 ttggaagaaa tcccctcttt ggaatcccat ctgcagttct gagtaaagag cagaatacta
      361 aaaattgtga taatatgaaa tttcatagtt catctccatt gttttctgta aatttgaggc
      421 agttcctgcc gccattgcag ggtaggcgtt cagttgaaat cctgttttct tttccatatt
      481 tccgaatccg agaaatcttt ttccggaaaa aaaggaatcc gtcagattca gaacatacac
      541 aagtccgaca aggagggcag cattctgaac atcgccggtc gccttcttca gctgactgta
      601 ttcagacttg tcctttgcaa agtagagaat ctctgcagga gatccgaaag gaatgatgag
      661 aaaggttctg tgaacagaat caaaatcttt ccttgcctta ctgtattctc cgttcctgta
      721 ggcaagatga agaagactga ctgcaaacag acttccgaca gcaattccct tgttcttatg
      781 ccacgaactt ccgttcttca ctccgtaatt gtactgtccc catcccggaa gaactgcaga
      841 acggacaagg gcttcatact tgttctcccc gcctttctga ccgatgacaa gatccgtata
      901 gtcggaaatt ccgcctgctg ccttttttgc aaaaagttca gaagcagact gaaatcctgc
      961 agtcgaatcc gccactgctg tttctgcaaa atcaatattg tcttttactg gatctattat
     1021 atttccattt atgaaatatt tatttcccac ctttgtgatg cttc
//
LOCUS       DAOQGJ010001885         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_122052_length_1063_cov_2.974206, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001885 DAOQGJ010000000
VERSION     DAOQGJ010001885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122052_length_1063_cov_2.974206"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PK683_22260"
     CDS             complement(<1..605)
                     /locus_tag="PK683_22260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577758.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNH11239.1"
                     /translation="MNKGKIKQVIGSVLDISFEGKLPEIFHALEIDVQKDGKSSVLVA
                     EVQQHLGDNTVRAIALSSTDGLVRGQEVRDTGESIMVPVGQPTLGRIFNVLGQPVDEA
                     GPVVSKDKRSIHVNPPKYEDLQPKTEIFETGIKVVDLLAPYIKGGKVGLFGGAGVGKT
                     VVIMELINNIAKQHGGFSVFAGVGERTREGNDLWREMKESGV"
     gene            complement(619..>1063)
                     /locus_tag="PK683_22265"
     CDS             complement(619..>1063)
                     /locus_tag="PK683_22265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNH11240.1"
                     /translation="IDDKVGYAEAEYFANLFKDSFAAGKVDLVEIISTVYHSSAAQKP
                     EVTQVLPLEPKAGTVPSNDMVLYEPSPERILESLIPMVVNMAFYKAVLEAVCSEQIAR
                     RIAMKSATDAAGEMIKILTRGYNRVRQAKITQEISEIVAGADSLK"
BASE COUNT          285 a          282 c          189 g          307 t
ORIGIN      
        1 accccggatt ctttcatctc tctccaaagg tcatttcctt cccttgttct ttcacccact
       61 ccggcaaaca cagaaaatcc tccgtgctgc ttcgcgatgt tgttgatcag ctccataata
      121 acgacagttt taccgacacc ggcaccgccg aaaagaccga cctttccacc cttgatataa
      181 ggagcaagaa gatcgactac cttaatccca gtttcaaaaa tctctgtttt tggctgtaga
      241 tcttcgtatt taggaggatt tacgtgaatg cttcttttgt ccttggaaac aacgggacct
      301 gcttcatcca ctggctgacc gagaacattg aaaatacgtc caagtgtagg ctgtcctaca
      361 ggaaccataa tagactcgcc agtatctctg acttcctgac cgcgaaccag tccatctgta
      421 gaagacagag caatcgcacg gacagtattg tctcccaaat gctgctgcac ttcagctact
      481 aatactgaac tctttccatc tttctgaaca tcaatctcca gagcatgaaa aatctcagga
      541 agttttcctt cgaaagaaat atccagtacc gatccaatta cctgcttgat tttgcccttg
      601 ttcatcttta ctcctaaatt atttcaaaga atctgcccca gcgacgattt cagaaatctc
      661 ctgggtaatt ttggcctgtc tgaccctatt gtatcctctt gtaagaatct tgatcatttc
      721 acctgcagca tccgttgcag acttcattgc gattcttctg gcaatctgtt ctgaacagac
      781 agcttccaga acagctttat aaaatgccat attgacaacc atcggaatga gcgattccag
      841 aatcctttcg ggacttggtt cataaagaac catatcattt gaaggaactg ttcccgcctt
      901 cggctccagc ggcagaacct gggtcacctc aggtttttga gccgccgatg aatgatatac
      961 tgtagatata atctctacca aatccacttt accggctgca aatgaatcct tgaaaagatt
     1021 ggcaaagtac tctgcctctg cataaccgac tttatcgtca ata
//
LOCUS       DAOQGJ010001886         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_122069_length_1063_cov_2.786706, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001886 DAOQGJ010000000
VERSION     DAOQGJ010001886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122069_length_1063_cov_2.786706"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PK683_22270"
     CDS             <1..349
                     /locus_tag="PK683_22270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11241.1"
                     /translation="CKIENHSSRNDSALVKKLLVDTGSEYSWIFESTLNSIGVSIEKK
                     DVPFKMANGTLITREIGFVIFRYDKYFTIDEVVFAKKGDLQLLGARTLEGLNLLVDTQ
                     KKKLVAAGPSVAA"
     gene            complement(560..>1063)
                     /locus_tag="PK683_22275"
     CDS             complement(560..>1063)
                     /locus_tag="PK683_22275"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNH11242.1"
                     /translation="DERLKKNIQPLGNFLEKLTELRPVTFEWKDPKKLNAKDGKHIGF
                     IAQEVEKVFPHWVDTDKDGMKWLSMEGVNAAFVQSFKEVAFRLRSTNDSVSAVTQKIE
                     SLEKEKAANEKKISALEADNKRLHELVETLNASVGTHGRTSQRMEDRLNALERMQIAT
                     AAQAARK"
BASE COUNT          257 a          265 c          204 g          337 t
ORIGIN      
        1 ttgcaaaatc gaaaatcatt ccagcagaaa tgattctgct ctcgttaaaa aattgctcgt
       61 tgatactggc agtgagtatt cctggatttt tgaatcgact ctgaatagca tcggtgtttc
      121 catagagaaa aaggatgttc catttaaaat ggccaacgga actctgataa cgcgggaaat
      181 cggatttgtg atcttccgtt atgacaaata ttttacgatc gatgaagttg tttttgctaa
      241 aaaaggtgac ctgcagcttc tgggtgcaag gacactggaa ggattgaatc ttctcgttga
      301 tacacaaaaa aagaaacttg tcgcagctgg tccctctgta gcggcataat acatctgttt
      361 tcattataaa cggatatttc atctcctgtg tatccttcac cgccttacct aactctgctt
      421 ttcctgcaac accgcatttc tcggaatact ctcgcacctg tcatttcgaa cagcattcgg
      481 tgatcttgcc gcaccgcaaa gtctgctgtg agaaatctta aatcattagg agcagttgac
      541 tttctcacac tgatccccat tatttcctcg ccgcttgcgc cgctgtcgct atctgcattc
      601 tttccaatgc attcagacga tcttccatcc gttgggacgt gcggccgtgc gtccctacgg
      661 atgcattcag cgtctcaact aactcgtgca gtctcttatt gtcagcttcc agtgccgata
      721 ttttcttctc gttagccgct ttttcctttt ccagagattc aatcttctgt gtaacagcac
      781 tgacagagtc gttcgttgag cggagccgaa acgcaacttc tttgaatgac tgcacaaaag
      841 cagcattaac cccttccatg gacagccact tcattccgtc cttgtccgta tccacccaat
      901 ggggaaatac tttctctact tcctgtgcaa taaatccaat atgttttcca tccttggcgt
      961 ttaacttctt gggatctttc cattcaaatg ttacggggcg taattctgtt aacttctcca
     1021 aaaagtttcc tagcggttgg atattcttct ttagccgctc atc
//
LOCUS       DAOQGJ010001887         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_122081_length_1063_cov_2.647817, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001887 DAOQGJ010000000
VERSION     DAOQGJ010001887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122081_length_1063_cov_2.647817"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..>430)
                     /locus_tag="PK683_22280"
     CDS             complement(14..>430)
                     /locus_tag="PK683_22280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11243.1"
                     /translation="QKKSIKKLEKEKESKDKIINMKDAELRRKAEMLQTIEEDYKSKF
                     DALSKGQPLPEKNRETKQLIMELMTTVNQISSELIEIPAEERDAELSNQTAMAIGLLR
                     RAANQLSDDWNNDLHDAEISSSRDPKFDVDLKELED"
     assembly_gap    431..440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<441..840)
                     /locus_tag="PK683_22285"
     CDS             complement(<441..840)
                     /locus_tag="PK683_22285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11244.1"
                     /translation="MSSEMTISQTAELSTEERSERARKAFILIEQVRVAGYTTAMALK
                     QLRDDKVHELLSYEDFEEFCDNELPFLKPKQISKYLQIADTFGDRLSVKEMLAKRGGM
                     TALLEVAEEIKVKGIEPSSLSDEDIEDIILR"
     gene            complement(837..>1063)
                     /locus_tag="PK683_22290"
     CDS             complement(837..>1063)
                     /locus_tag="PK683_22290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11245.1"
                     /translation="WTIEELRICVRIHEKSLYRAVKKLRDHKIIIKSGTIYMLNADII
                     GSLRANIVELQKQRDRSKYLQIQKQRGETK"
BASE COUNT          206 a          262 c          209 g          376 t
ORIGIN      
        1 gcacctttca cggtcagtcc tccagttcct tcagatcaac gtcgaatttc ggatctctgg
       61 aggaagagat ttcagcatcg tgcaggtcat tgttccagtc atcggaaagc tgatttgccg
      121 cacgtctcag aagtccaatc gccattgccg tctgattgct cagttctgca tctctttcct
      181 ctgccggaat ttcaatgagt tccgaactga tctgattgac tgttgtcatc agctccatga
      241 tgagctgttt tgtctccctg ttcttttccg gcaggggctg gcctttgctc agtgcgtcaa
      301 atttggattt ataatcttct tcaatcgtct ggagcatctc agccttgcgt ctgagttcgg
      361 catctttcat attgatgatt ttatctttac tttctttttc tttttcgagt ttcttgatgc
      421 tcttcttttg nnnnnnnnnn gtctgaggat aatatcttca atgtcttcat cactcagaga
      481 ggatggttca atgcccttga ccttgatttc ttctgcaact tcaagcaggg cagtcatccc
      541 gccacgcttg gcgagcatct ctttcacgga aagcctgtct ccgaatgtgt ctgcaatctg
      601 caggtatttt gaaatctgtt tcggtttcaa aaacggaagt tcgttgtcgc agaattcctc
      661 aaaatcttca tagctgagaa gctcatgaac cttatcatct ctcagctgtt tgagtgccat
      721 tgctgtggtg tatcctgcaa ccctcacctg ttcaatcagt atgaatgctt ttcttgctct
      781 ctcacttctt tcctctgtgg aaagttctgc cgtctgcgat attgtcattt cactgctcat
      841 ttggtttctc ctctttgttt ctgaatctgt aaatatttac tgcggtctct ctgcttctgc
      901 agttcgacaa tgtttgccct cagtgaaccg atgatatctg cattcagcat atatatggtt
      961 cctgatttta taatgatctt atgatccctc agtttcttga ctgcccgata caggctcttc
     1021 tcgtgaatgc ggacacagat cctgagttcc tcaatcgtcc agc
//
LOCUS       DAOQGJ010001888         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_122117_length_1063_cov_2.357143, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001888 DAOQGJ010000000
VERSION     DAOQGJ010001888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122117_length_1063_cov_2.357143"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PK683_22295"
     CDS             complement(<1..555)
                     /locus_tag="PK683_22295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11246.1"
                     /translation="MANVKNELAEWFIRQDHNYFNRVFKDNKNKLISDLDSYNEQLNI
                     GDIFECDAVNYKEVISKISKEIYEKKIKIDSFFQGKKGDIPRAILGKDNYFKFVREKF
                     GSKEGEIMDVSESNKASKENKFPMNQILYGPPGTGKTYNTMNKAIQIINPEFFLGNRT
                     EVRAEYERLVREKQITFTTFHQSMS"
     gene            693..>1063
                     /locus_tag="PK683_22300"
     CDS             693..>1063
                     /locus_tag="PK683_22300"
                     /inference="COORDINATES: protein motif:HMM:NF013853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNH11247.1"
                     /translation="MQTLVDMKKLYEEDEHLWLFENARLLREGRLDLADIQHIAETLE
                     DMGKRDFREILSRLIILIGHLLKWKFQQEQRSSSWKGTINEQRLKLNLEFEDSKNLEN
                     YGRENFPAAYKKARSLAASDTG"
BASE COUNT          348 a          192 c          182 g          341 t
ORIGIN      
        1 actcatcgac tgatgaaaag tagtaaatgt aatctgtttc tcccgaacca gtctttcata
       61 ttcagctctt acttctgtcc tgttacccag aaagaactcc ggatttatga tctgaattgc
      121 tttgttcatt gtattatatg tttttccagt tccaggaggg ccataaagga tttgattcat
      181 agggaattta ttttctttag aagctttatt tgattctgat acatccataa tttcaccttc
      241 tttactgcca aatttttcac gtacaaattt aaaataatta tctttaccaa gaatagccct
      301 tggaatatct cctttcttac cttgaaaaaa agagtctatt ttgattttct tttcatagat
      361 ttctttacta atttttgaaa taacttcttt gtagtttacc gcatcgcact caaatatatc
      421 acctatattg agctgttcat tgtaactgtc caaatcactt atcaatttgt ttttattatc
      481 tttgaaaact cgattaaagt aattatgatc ctgtctgata aaccattctg caagctcatt
      541 ttttacattc gccatagata atttccttat tcagtaagtg cgctctggta aattcgccaa
      601 gtacagtaca accgtaaaaa agtcagctgt caagctcaaa tccgctataa gattttcttg
      661 acagaaaata aagaccagaa taccataaga ttatgcagac cttagttgat atgaaaaaac
      721 tttacgagga agatgagcac ctctggctct tcgaaaatgc aaggcttctg cgggaaggca
      781 ggctggatct tgccgacata cagcatattg cggaaacact ggaagatatg ggaaaaagag
      841 attttagaga aattttaagc agactgatta ttctaattgg gcatctttta aaatggaaat
      901 tccaacaaga acagagatcc agcagctgga agggaacgat taacgaacag agactgaaac
      961 tgaatttaga atttgaagac agcaaaaatt tggaaaacta cgggagagaa aattttccgg
     1021 ctgcatacaa aaaagcaaga agtctggctg caagcgatac tgg
//
LOCUS       DAOQGJ010001889         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_122272_length_1062_cov_3.275074, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001889 DAOQGJ010000000
VERSION     DAOQGJ010001889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122272_length_1062_cov_3.275074"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PK683_22305"
     CDS             <1..848
                     /locus_tag="PK683_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11248.1"
                     /translation="DGRILFKIDSEYTEYPSAESQPEIHLEEAYSLFEKKKNVSALRI
                     LKGMELCFRNQTEDAKKRNMPLLHKTVELKNRILSLSEDRKEELNSESDPYLCRMQDY
                     HILESEEYGLRMKIPLQLSPRTGMQFISRGKWHNYKIMYFTAEDQIQKDFSMEEWLNF
                     LSKKKPYPLNKRIVLSLNFSRHRSDRVTETFLKNFWENERGLNDRQKSILKYVSEKRE
                     NAVYSEFFRNDNDGKFRKYSLYHTYRVSRNDRGVSVFLSAPEEREETVRIFWKTVFDS
                     VSMKN"
     gene            complement(845..>1062)
                     /locus_tag="PK683_22310"
     CDS             complement(845..>1062)
                     /locus_tag="PK683_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11249.1"
                     /translation="KLASKVDKLEKTTEDVQDELNAKRQARDEWFKWHFDWKNAWVSE
                     SENMTEEEKEKKKVETSVTSRYKALDI"
BASE COUNT          328 a          213 c          222 g          299 t
ORIGIN      
        1 gggacggcag gattctgttc aagatagatt ctgagtatac agaatatccc agtgccgaat
       61 cccagccgga gattcatctc gaagaagcat attccctttt tgaaaaaaag aaaaatgtgt
      121 ccgcacttcg gattctgaaa gggatggagc tctgtttcag aaatcagact gaagatgcaa
      181 agaaaaggaa tatgcctctc ctgcataaga ctgttgagct gaaaaacaga attctctctc
      241 tttctgaaga cagaaaagag gagctgaaca gtgaatcgga tccctatctc tgcagaatgc
      301 aggactatca tattctggaa tcagaagaat acggcctcag aatgaaaatc cctcttcagc
      361 tcagtcccag gacaggaatg cagttcatca gcagaggaaa gtggcataat tataaaatca
      421 tgtattttac ggcagaggat cagattcaga aggatttttc catggaagaa tggctgaatt
      481 ttctttcaaa aaagaaaccc taccctctca ataaaagaat tgtgctatca ctcaattttt
      541 cccgtcacag aagcgacagg gtcacggaaa cttttctgaa gaatttctgg gagaatgaac
      601 gcggactgaa tgaccggcag aagtccattt taaagtatgt ttcagagaaa cgggaaaatg
      661 ctgtttacag cgagtttttc cgaaatgaca acgacgggaa attcagaaaa tattcattat
      721 atcatacata cagagtatct cgaaacgaca gaggagtatc cgtgttctta agcgcaccgg
      781 aagaaagaga agagactgta aggatttttt ggaaaacggt ttttgattct gtttctatga
      841 agaattaaat atccaaagcc ttgtatcggc tggtcactga ggtttccact ttcttttttt
      901 ctttttcttc ttcagtcata ttttcagatt cagagaccca tgcgtttttc cagtcgaaat
      961 gccatttaaa ccattcatcc cgggcctggc gttttgcatt cagctcatcc tgaacatcct
     1021 cagtggtttt ctccagctta tcgactttgc ttgccaattt ct
//
LOCUS       DAOQGJ010001890         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_122384_length_1062_cov_1.967229, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001890 DAOQGJ010000000
VERSION     DAOQGJ010001890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122384_length_1062_cov_1.967229"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK683_22315"
     CDS             complement(<1..220)
                     /locus_tag="PK683_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNH11250.1"
                     /translation="MNEIQEEFEELTSEFLFGIITPEKEIKLKSILSTNPELEMKFRE
                     YLKLHSRIVNSEEIWENENSEPGRKTVPF"
     gene            complement(213..722)
                     /locus_tag="PK683_22320"
     CDS             complement(213..722)
                     /locus_tag="PK683_22320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH11251.1"
                     /translation="MKDLSFFEKQFIAYRGKILSFFKARAAFEDAEELSQETYLRAFK
                     SIEKFDSSKGEFGSWLFAIARNVFIKHKSKDYLTEADMESSLIPDRYSLENEFQKKSL
                     TETVKLCISCLPEPEKGILISKYKYNKSLEEIGAEFGISARTVSRKYLEALNLLKEEL
                     RKKGVDGNE"
     gene            851..>1062
                     /locus_tag="PK683_22325"
     CDS             851..>1062
                     /locus_tag="PK683_22325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11252.1"
                     /translation="MNGSEHRFIPSKEKAKALMILLPCMGGNILAYRYPYSLWSKLGY
                     NMFLFNPPGHGKSDGIFSFEESFRLL"
BASE COUNT          283 a          221 c          184 g          374 t
ORIGIN      
        1 aaaaaggtac agttttcctg ccgggttctg aattctcatt ttcccaaatt tcttcggagt
       61 ttacgattct gctgtggagt ttaagatatt ccctgaattt catttccaat tcaggatttg
      121 tgcttaaaat ggatttcagt ttgatttctt tttcaggagt aatgatgccg aaaagaaatt
      181 cggaagtcag ctcttcaaat tcttcctgta tttcattcat ttccatccac tccttttttt
      241 ctcagttctt ccttcagaag attcagtgct tccagatatt tcctgctgac cgttcttgca
      301 gaaattccga actctgcgcc aatttcttca agtgacttat tgtatttgta tttactgatt
      361 aatatccctt tttcgggttc aggcagacag gaaatacaga gttttaccgt ttcggtcaga
      421 ctttttttct ggaattcatt ttccagactg tatctgtccg gaataagaga tgattccata
      481 tcagcctcag tcagataatc tttggactta tgttttatga atacatttct tgcaattgca
      541 aaaagccagc ttccaaactc ccctttggag gaatcaaatt tttcaatcga cttgaacgct
      601 cttaaataag tttcctgact cagttcttct gcatcttcga aagctgctct ggctttaaaa
      661 aaggagagta ttttccctct gtatgcaatg aactgtttct caaaaaaact gaggtctttc
      721 acaggacaaa agaaaataca tatcataaac tgagcaagat atattttata tgaagtctga
      781 tcctggatga ttttgggcat ccggggattt ttcattgact gtcactgttc ctccccatat
      841 tgctggttat atgaacggat cggaacaccg atttatccct tcgaaagaaa aggcaaaagc
      901 acttatgatt ctgcttccct gtatgggcgg aaatattctc gcatacaggt acccatattc
      961 cctctggagc aaactgggat acaatatgtt tctttttaat cctccaggac acggaaaatc
     1021 cgacggtatt ttctcttttg aagaatcatt ccgtcttctt aa
//
LOCUS       DAOQGJ010001891         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_122633_length_1061_cov_1.703777, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001891 DAOQGJ010000000
VERSION     DAOQGJ010001891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122633_length_1061_cov_1.703777"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK683_22330"
     CDS             <1..399
                     /locus_tag="PK683_22330"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH11253.1"
                     /translation="KQYQRKVKTGKVFDSSGGFVLPNKAMRSPDAAWISTERWETLTA
                     EEKRKFPPLCPEFVIELLSESDDLYRTKKKMEEWMENGCRLGWLIDPFQKNVYIYKEF
                     LQMKTQGFDMVLSGEDILPGFELPLNELDD"
     gene            complement(405..>1061)
                     /locus_tag="PK683_22335"
     CDS             complement(405..>1061)
                     /locus_tag="PK683_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11254.1"
                     /translation="EYVVVKKIRKNDKISIALRPGLEIEAKSKTKKFKGIIESRDTNK
                     GSINLKTKTAEVETLTDKDITEIRWENEEIKFSTKFLRLPKYLIPGIVQFPENRWKGA
                     GMFTAFLLFAAAIPIEYSAAQSSLKNDSTILPFNGTFIVVSGFGSNPAFETHQRNMMA
                     AGAGLGLLYAYHFYDVWKMNQPKEKGTTFDFRLTPQYNFQSDAVKVTPGAEFRFNYNF
                     "
BASE COUNT          289 a          210 c          238 g          324 t
ORIGIN      
        1 aaacagtatc agagaaaggt gaagacagga aaagtttttg attcttccgg cggttttgtg
       61 cttccaaaca aagcgatgcg ttctcctgat gcagcctgga tttccactga aagatgggaa
      121 actctcacag cagaagaaaa gaggaaattt ccgcctctct gtccggaatt tgtgatcgaa
      181 cttctttctg aatctgatga tctttatcgg acaaaaaaga aaatggaaga gtggatggaa
      241 aatggatgca gactcggctg gctcattgac ccgtttcaga aaaatgttta tatttacaag
      301 gagttccttc agatgaaaac ccagggtttc gatatggtat tgtctggcga agacatcctc
      361 cctggttttg aactcccctt aaatgaactg gatgactgat cctttcagaa attataattg
      421 aatcggaact ccgctccggg agtgaccttg actgcatccg actggaaatt gtactgaggg
      481 gtcagcctga aatcaaaagt tgttcctttc tctttaggct gattcatttt ccagacatca
      541 taaaaatggt aagcatagag aagacctaga cctgcacctg cggccatcat atttctctgg
      601 tgagtttcga atgcaggatt gcttccaaac ccggacacaa caataaatgt tccgttgaaa
      661 ggcagtattg tagagtcatt cttcagtgag gactgagcgg cagagtattc aatcggtatt
      721 gcggcagcaa aaagcagaaa ggcagtaaac atacctgctc ccttccagcg gttttccgga
      781 aattgaacaa ttccaggaat cagatatttg gggagacgaa gaaactttgt actgaatttg
      841 atttcctcat tttcccatct gatttcagtg atatccttgt ctgtaagagt ttctacttct
      901 gcagttttgg tcttcaggtt gatggatcct ttgtttgtgt ctctggattc gattatgcct
      961 ttaaattttt tcgttttgga ctttgcttca atctcgagac ccggtcggag ggcaatggag
     1021 attttatcat ttttcctgat ttttttgacg acaacgtatt c
//
LOCUS       DAOQGJ010001892         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_122665_length_1060_cov_5.438806, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001892 DAOQGJ010000000
VERSION     DAOQGJ010001892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122665_length_1060_cov_5.438806"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..>1060)
                     /locus_tag="PK683_22340"
     CDS             complement(30..>1060)
                     /locus_tag="PK683_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11255.1"
                     /translation="TGVMQDNASPFNIADELNIPYFSDEETAELLGQHETETGQIFES
                     SVKKKISEITANQPGLVNGFAKKLVDTNPEKSVISSADFETAREWYLNKAIDKNIANI
                     IKIAEQQRKFLERLLFDEEEIPFEIDRPVIKILHVNGLITYDEKNNVRFWVPLYRERL
                     FKALYPYSNGESKRISESMLANSYLTKEREINFDRLMESYRNHIRSRSFRPFRERDEQ
                     GNFISIPEAAMIYSFETFISIFVQELEGKIYRESIVSLGNSDMVIHAEGREYFIEVKK
                     YYSPRKFEKGKKQLAYYVKRAGLQQGVYIVFLRNNLYEDLPDNSSEIIDNIIIKTYII
                     RYDEEKDF"
BASE COUNT          258 a          231 c          192 g          379 t
ORIGIN      
        1 taagtgcatt ccggatctgc cttccagccc taaaaatcct tctcttcatc atatctgata
       61 atatacgtct ttataattat attatctata atttccgaac tgttgtccgg aagatcttca
      121 tacagattgt ttctcaaaaa aacaatatag actccctgct gaagtcctgc tctttttaca
      181 taataggcaa gctgcttttt tcctttttca aatttacggg gactgtagta ttttttcact
      241 tcgatgaagt attctcttcc ttctgcatgg atcaccatat ctgaatttcc cagggatacg
      301 atggattctc tgtatatttt cccttccagt tcctgaacaa atatgctgat gaatgtttcg
      361 aagctgtaga tcattgctgc ttcaggtatg gagatgaaat tcccctgttc atctctttca
      421 cggaatggcc gaaaacttct ggatcggata tgatttctgt aactttccat cagcctgtca
      481 aaattgattt ccctctcttt cgtcaaatag gaattggcaa gcatggactc cgatatccgc
      541 ttggattcac cgttgctgta aggatagagt gccttgaaaa gacgttctct gtacagtgga
      601 acccagaacc ggacattatt tttttcatca taggtaatga gtccgtttac gtgaagaatc
      661 ttaatgactg ggcggtctat ttcaaacggg atctcttctt cgtcgaaaag cagtctttcc
      721 agaaattttc tctgctgttc tgctatctta atgatatttg ctatattttt atctattgcc
      781 ttattcagat accattctct tgccgtctca aaatctgctg atgagatcac agatttttcc
      841 ggatttgtat ccaccagttt tttggcaaag ccgttgacca gtcccggctg attggctgtg
      901 atctcgctga tctttttctt cacggaagac tcgaagatct gtcctgtttc tgtttcgtgc
      961 tgtcctaaaa gttctgctgt ttcttcgtct gaaaaatagg gaatattcag ttcatctgca
     1021 atattgaaag gacttgcatt gtcctgcatc acacctgtga
//
LOCUS       DAOQGJ010001893         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_122950_length_1059_cov_2.905378, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001893 DAOQGJ010000000
VERSION     DAOQGJ010001893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122950_length_1059_cov_2.905378"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PK683_22345"
     CDS             <1..351
                     /locus_tag="PK683_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11256.1"
                     /translation="RHDTEASWVYELTLYEKNEKKLIIMNSASRKGVWSFMPFYIGTV
                     STVLASALDRHRLPSNLQKYCLEAPGKLREFFEQKREDNCREYSEHLYQSLYLASPVW
                     RKMIRNRDHEFVIK"
     gene            811..>1059
                     /locus_tag="PK683_22350"
     CDS             811..>1059
                     /locus_tag="PK683_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11257.1"
                     /translation="MNFKSDLNPKTELNPTQLQAVSDQVSSGLGRQGVYKVLDRVSSK
                     ERLKEIADQQRGLYDESKTIRASQSLGADKILLGSVTKL"
BASE COUNT          314 a          220 c          226 g          299 t
ORIGIN      
        1 cggcatgata ctgaggcatc ctgggtatat gaactgactc tgtacgaaaa aaatgaaaaa
       61 aaattgatta taatgaattc tgcctcccgc aagggtgtct ggtctttcat gcctttttac
      121 ataggcacag tcagcactgt tcttgcctcc gcactcgaca gacacagact tccgtcaaat
      181 cttcagaaat actgtctgga agctccagga aaactgagag agttttttga gcagaaaaga
      241 gaagacaact gcagggaata ttctgaacat ctttatcaga gtctgtatct tgcttctccg
      301 gtctggagaa aaatgatccg gaacagagat catgaatttg taattaaata atatttatct
      361 gtttcgaagg gccagatcgg ttcactgcgt caagcattcc gcaggaatca gcattgtatg
      421 atgatttaag atttctcttc acaaatcggg aagctgttca tcgaaatgac agattggaca
      481 agccggtgat gggagattca agtttaataa actcataaac tatatgtgtt tatacctatt
      541 ttgccgttcc tgcaaaagta cgcagttctg cgtattgcac gtaaatgcct gtctgaaatt
      601 cacggtcaaa atgacacctc cgattcaata aaaatgattt gccatttttt actcagcctt
      661 ccttctgtgg gcattatgaa atttttcaga tattattcag acagacattt ccataaatcc
      721 gcttactgta actttctctt agtttcagta atctttatat ttttctgcct tcctgtttca
      781 gcacaggcgg aaaaaccgac tcttaccgtt ttgaatttca aatcagactt gaatcccaaa
      841 acggaactga acccgacgca gcttcaggca gtcagcgatc aagtgagcag cggtctaggc
      901 agacagggtg tttataaagt tctggacagg gtttcgagca aggaacgcct gaaggaaatc
      961 gcagaccagc agcggggact ctatgacgaa agcaaaacaa tccgtgcgag tcagagtctt
     1021 ggagcggata aaattctgct cgggagtgtc acgaagctt
//
LOCUS       DAOQGJ010001894         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_123167_length_1058_cov_2.889332, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001894 DAOQGJ010000000
VERSION     DAOQGJ010001894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123167_length_1058_cov_2.889332"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK683_22355"
     CDS             complement(<1..282)
                     /locus_tag="PK683_22355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH11258.1"
                     /translation="MEEKGFRILAVDDEQVNLNVLENQLSLEGWSVISAENGETAVHL
                     VESSLPNLILLDVMMPKISGYEVCRIIRKKYTQAELPIIMLTAKNTENDL"
     gene            631..>1058
                     /locus_tag="PK683_22360"
     CDS             631..>1058
                     /locus_tag="PK683_22360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11259.1"
                     /translation="MYFPFFSKFDRIAQLAICSVALLGSLATMQLPAVAVPAAEIGRL
                     AESDSGLRPPLKEGLKYSYGKEALSEFSRAVSNGKKAIDQALLKANGDAGKLCIVSDI
                     DETLLDNRPFIEREVAASDKEVNWSQFEGWVQEASGKLLKP"
BASE COUNT          243 a          275 c          231 g          309 t
ORIGIN      
        1 aagatcattt tcggtatttt ttgctgtcag catgatgatc ggcagttctg cctgtgtata
       61 tttttttcta ataattctgc atacttcata tcctgaaatc ttaggcatca ttacatccag
      121 aagaatgaga ttcggcaggg agctttcgac cagatgcact gcagtctctc cgttttctgc
      181 cgagatcaca gaccatcctt ccagactgag ctgattttcc agaacattca ggttcacctg
      241 ttcatcatcg actgcaagaa ttctgaaacc tttctcttcc acagttttct gttcttttct
      301 ttctataatg atattttctg tattctgcag cctgttgtat tccttgaaag aaggtgctcc
      361 tgatgatgaa cctggaactg caggtatggt gaatatcagc ctgcttcctt ttccgagttc
      421 ggactctgcc ctgatttttc ctccgtgaag ctcaaccagc tttttcgtaa ttgcaagacc
      481 gattccggtt cccccgtatt ctctggcaac agaagcatct gcctgttcga aggatttgaa
      541 aatttcctcc agtttgtctt ctgcgattcc tatcccggta tctgagacgg cgatctctgc
      601 cgtttcattt tccagaagtc tggctgagat atgtattttc cctttttcag taaatttgac
      661 cgcattgccc agttggcaat ctgttccgtt gctctgctag gcagtcttgc caccatgcaa
      721 ttaccggcag tggcagtacc tgccgccgaa atcggccgcc ttgccgaaag cgacagcggc
      781 ctgaggccgc ccctcaagga aggcctcaaa tacagctacg gcaaggaagc tttgagcgaa
      841 ttcagccggg ctgtatccaa cggcaaaaag gccatcgacc aggctttgct caaagccaat
      901 ggtgacgccg gcaaactctg cattgtctcc gacatagacg aaacattgct cgacaaccgc
      961 ccttttatcg agcgtgaagt agccgccagc gataaagaag ttaactggtc tcaatttgaa
     1021 ggctgggtgc aggaagcctc cgggaagcta ctgaaacc
//
LOCUS       DAOQGJ010001895         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_123548_length_1056_cov_3.426573, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001895 DAOQGJ010000000
VERSION     DAOQGJ010001895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123548_length_1056_cov_3.426573"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK683_22365"
     CDS             complement(<1..251)
                     /locus_tag="PK683_22365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11260.1"
                     /translation="MSLFKKEFLNEVGICLDQRETEGNLDKAITLLMDLKAKYPNEDI
                     ICGKLSSAYFYKALFETNPKRQELFYEHGVNYGREAATL"
     gene            complement(403..>1056)
                     /locus_tag="PK683_22370"
     CDS             complement(403..>1056)
                     /locus_tag="PK683_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNH11261.1"
                     /translation="ANVVNNDKKVDGRIKVVFLPNYRVSLAEKIFPASDVSEQISTAG
                     TEASGTGNMKFALNGALTIGTLDGANIEIMEEVGTDNIYIFGLKTEEVQSWKKTGYSP
                     SQIIGAHAELGRIFTLIKENFFSPNSRDLFNPIHDSLFYDDVYMLIADYQSYADTQAK
                     IGKDYADKAGWTKKSIMNVAGMGKFSSDRTIKQYADEIWKVKQQTPAETKYKFKLMK"
BASE COUNT          253 a          244 c          191 g          368 t
ORIGIN      
        1 ttcagagttg ctgcctctct tccgtagttg actccatgtt cataaaagag ttcctgacgt
       61 ttcggatttg tttcaaaaag agccttgtaa aaataggcac ttgaaagctt tccgcagatg
      121 atatcttcat tgggatattt tgccttcaga tccatcagaa gtgtaattgc cttgtccaga
      181 tttccttcag tttctctctg atcgaggcag atacccacct cattcagaaa ttctttttta
      241 aataaactca ttcactgtct cctagagtgt ttttttgcat ctgccagacc tttgcgccgc
      301 agatgctttt ttcattcaat tcaataaggg ttgtgcaaaa gcaaggttat cgaaggtact
      361 tttacacagc ctctcaattt ctgttttctc agtctcagaa aattatttca tcagtttgaa
      421 cttgtatttt gtttcggcag gtgtctgctg ctttaccttc cagatttcat ctgcatactg
      481 tttgattgtc ctgtccgatg aaaattttcc cattcctgcc acattcataa tggacttttt
      541 ggtccatccg gccttgtcgg cataatcttt tccaatcttg gcctgagtgt ctgcatacga
      601 ctgataatca gcaatcagca tgtacacatc atcataaaaa agactgtcgt gaataggatt
      661 aaaaagatct ctgctgttcg gtgaaaagaa gttttccttg atcagagtga atattcttcc
      721 cagttctgca tgagcgccta tgatctgcga aggtgaatat cctgtctttt tccagctctg
      781 aacttcttct gtcttcagtc cgaaaatgta gatattgtca gttcctacct cttccataat
      841 ctcaatattt gccccgtcca gagttccgat tgtcagtgcg ccgttcagag caaacttcat
      901 gtttcctgta ccgctcgcct ctgttcctgc agtggaaatc tgctcagaga catcagatgc
      961 agggaatatc ttttctgcaa gagataccct gtagttcgga aggaatacta ctttaattct
     1021 tccgtcgacc tttttatcat tgttcactac attcgc
//
LOCUS       DAOQGJ010001896         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_123584_length_1056_cov_2.683317, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001896 DAOQGJ010000000
VERSION     DAOQGJ010001896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123584_length_1056_cov_2.683317"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..487
                     /locus_tag="PK683_22375"
     CDS             113..487
                     /locus_tag="PK683_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11262.1"
                     /translation="MNPEETLLLQVLAVSVKTLGEELVKSGEYDAHHPVRSTLKSADE
                     YANNPTEENWNQFFSDSTDSYPFGPGDGCYSIVSGCEAGSGCRSGAGSLLFTLESIPG
                     LTEEKAISLIHHRIELLSTGEL"
     gene            564..>1056
                     /locus_tag="PK683_22380"
     CDS             564..>1056
                     /locus_tag="PK683_22380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11263.1"
                     /translation="MTEKPGMEFEKISHLLNTEESELKYLLKLEEEEIGHLRMKVGSA
                     ILEEQSGIWEPLAKVTKFMPNFVNAKISEEVLGPKISANITYHLSASDAVSISGFMSA
                     KFFCDVLEHLIPEKIEFIIRATPLEQMRKAMNELKRRKNYFLIGSLIDYTPVPSVEKL
                     SKEM"
BASE COUNT          314 a          194 c          261 g          287 t
ORIGIN      
        1 agtggattga aattcagtga aaaccctgga ggaaattctg gaaaaaactt tctatttctc
       61 ttcactcgga tccctccgga attttatttc atggctggat aatgaaattt gcatgaatcc
      121 ggaagaaact cttcttctgc aggtgcttgc cgtttctgtg aaaacattgg gagaggagct
      181 tgtaaaatca ggagagtatg atgcacatca tccggtcagg tcaactttga aatccgcaga
      241 cgaatatgca aataatccga cagaggaaaa ctggaatcag tttttcagcg attccactga
      301 ctcttatcct ttcgggccgg gggatggatg ttattccata gtttcgggat gtgaagcagg
      361 cagcggatgc agaagcggtg caggctctct tcttttcacg ctggaaagca ttccaggact
      421 cacggaagaa aaagcgattt cacttattca tcatagaata gaattgctgt ccacaggtga
      481 attgtaggaa ctccatcctg aaaaagaatt tccttttaga ggggaaagtg tatcctagtg
      541 cgtaaagcat aaagagaggc agaatgacag aaaaaccggg gatggaattt gaaaagatca
      601 gtcatctttt gaatactgag gagtctgagc tgaaatacct tttgaagctc gaagaagagg
      661 agatcggtca tttgaggatg aaagtgggaa gtgcaatcct ggaggagcag tccgggatct
      721 gggaacctct ggcgaaggtg actaagttta tgccgaattt tgtgaatgcc aaaatctcag
      781 aagaagtcct tggtccaaaa atttcagcga acatcacata tcatctttct gcttctgatg
      841 cggtcagtat ttccgggttt atgtctgcaa aatttttctg cgatgtgctg gaacatctga
      901 ttcctgaaaa gattgaattt atcatccgtg caactcctct tgagcagatg cgcaaagcga
      961 tgaatgagct gaagagaaga aaaaactact ttctgattgg aagtctgatt gactatactc
     1021 ccgttccgtc ggtggagaaa ctttctaaag agatgg
//
LOCUS       DAOQGJ010001897         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_123622_length_1056_cov_2.328671, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001897 DAOQGJ010000000
VERSION     DAOQGJ010001897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123622_length_1056_cov_2.328671"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PK683_22385"
     CDS             complement(<1..619)
                     /locus_tag="PK683_22385"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNH11264.1"
                     /translation="MRYILIIFAFFVTALHSFDQSHSKFDGLLKKYVQNGLVSYIGFQ
                     KDREGLKKYLEELSSVSQSEYNGFSREDKMAFLINAYNAYTIELILKNYPLKSIRKIG
                     FLPGAAWKEKFFRLLGEERNLDWIEHSRLRVDFGEPRIHFTVVCASIGCPHLQSEVYT
                     GKNLSTQMENAAARFLNDSKKNYYSEKDNTLYISSIFDWFRKDFEK"
     gene            complement(616..>1056)
                     /locus_tag="PK683_22390"
     CDS             complement(616..>1056)
                     /locus_tag="PK683_22390"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11265.1"
                     /translation="AKTFKADYRVMPRTVYTDPEIARVGISEKEALENGIEYEKTEYD
                     IGELDRAVTEGEARGFIQVLTEKRSDRILGVTIAALSAGEILAEYVLAMKHGIGLNKI
                     LGTVHSYPTMSEANKYAAGNWKKKHKPEKLLNFLQKFHSWRRGL"
BASE COUNT          263 a          258 c          193 g          342 t
ORIGIN      
        1 gcttttcaaa atcctttctg aaccagtcga agatggaaga tatgtacaga gtattgtcct
       61 tttcagaata ataatttttc ttactgtcat tcaggaacct tgcagccgca ttttccatct
      121 gcgtgctcag attttttcca gtatagacct cgctttgaag atgagggcat cctattgatg
      181 cgcatacaac agtaaaatgg attctcggtt ctccaaaatc caccctgagc cttgagtgtt
      241 caatccagtc cagattcctt tcctccccga gaagtctgaa gaatttttcc ttccaggctg
      301 cccccggaag aaagccgatc tttctgatgc tcttcagagg ataattcttc agaataagct
      361 caatcgtata tgcattgtat gcattgatga gaaaagccat tttatcttca cggctgaaac
      421 cgttatattc ggactgactg acggatgaaa gttcttccag atattttttc aggccttccc
      481 tgtctttctg aaatccgatg taactgacaa gtccgttctg aacatatttc ttcagaagac
      541 cgtcaaactt cgaatgactc tgatcgaatg aatgcagtgc tgttacaaaa aatgcaaata
      601 taattaatat gtatctcata atcccctcct ccatgagtga aacttctgca gaaagttcag
      661 aagtttttcc ggtttgtgct tctttttcca gttccctgcg gcatacttat tcgcctcgga
      721 catagtggga tatgaatgca cggttcccag aatcttattc agaccgattc catgtttcat
      781 tgcaagaaca tactctgcaa ggatttctcc ggcagaaaga gctgctatcg tcactccgag
      841 tatcctgtca ctccgctttt cagtaagcac ctgaataaag cccctggctt ctccttccgt
      901 tactgccctg tccagttcac ctatgtcata ttcggttttt tcatactcaa ttccgttttc
      961 cagtgcctct ttttcggata tgcccactct ggcaatttcc ggatcagtgt atacagtccg
     1021 aggcatcact ctgtaatctg ccttgaatgt ttttgc
//
LOCUS       DAOQGJ010001898         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_123737_length_1055_cov_3.593000, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001898 DAOQGJ010000000
VERSION     DAOQGJ010001898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123737_length_1055_cov_3.593000"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..>1055
                     /locus_tag="PK683_22395"
     CDS             329..>1055
                     /locus_tag="PK683_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNH11266.1"
                     /translation="MSGHSKWATIRRKKEAIDSKRGAVFTRMGKELSVAAKMGGSDPE
                     ANPRLRVAILKAKSVNMPKDNIERAIKKGAGELDGVTYEECLYECFAPGGVAIMVQVL
                     TDKKSRTTPEIKNILTKAGGSLATSGSVSRLFERKGFLSLKSDLISEEDLFELAVGAG
                     AEDVQKEDDVFIITTAPEDYESVLTALSKKNLQMVESEVRFIPLVKAEVSDQESAEKV
                     MKLLDNLEGHDDVQSVNSNFEPSN"
BASE COUNT          323 a          174 c          258 g          300 t
ORIGIN      
        1 tagaagagaa atctgtactt caggagatcc tgtatccgat gcatgtcttt tgttatcaga
       61 aattaaagtc tgtttttgtt cttttgtaat catagttgta ctcaaattaa ccagataatt
      121 ttagcgcatt ttgagagaaa gaaatttttg cttttattcc tttgaataat tccgatgagt
      181 cgttcagttt ggacagtatg tctaaaaatc tctttcaaaa aaagcaggga ttttttttat
      241 agggatacgt tggaactgtg taatcgggca agtttgcaca ggctaatctt aaatcattca
      301 ctcagatgga gttttcatag aggtttttat gtcaggtcac tcgaaatggg caacaatacg
      361 cagaaaaaaa gaagcaatcg attctaagag aggagccgtc tttaccagaa tgggaaagga
      421 actttccgta gcggcaaaaa tggggggttc tgatccggaa gccaatccaa gactcagggt
      481 ggcaattctg aaggcaaaat cggtgaatat gcctaaggac aatatagaac gtgccattaa
      541 aaagggagca ggcgaactgg atggggtgac ttatgaggaa tgtctgtatg aatgctttgc
      601 gccgggcggg gttgcaatta tggttcaggt tctcactgat aaaaaatcca gaacaactcc
      661 tgaaattaaa aatatactga ccaaggcagg cggttctttg gcaacatccg gaagtgtcag
      721 cagacttttt gaaagaaagg gttttttaag cctgaaatcg gatctgattt cagaagagga
      781 tctttttgaa ctggctgtgg gggccggagc tgaagatgtt cagaaagagg atgatgtatt
      841 tatcattacg actgcacctg aagactatga atctgttctc actgcactca gcaaaaaaaa
      901 tctgcagatg gtggagtctg aggtccgttt cattcccctt gtaaaggctg aggtcagtga
      961 tcaggaatct gcggaaaagg taatgaaact tctggacaat ctggaaggac atgatgatgt
     1021 gcagagtgtc aattccaatt tcgaaccttc gaatg
//
LOCUS       DAOQGJ010001899         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_123971_length_1054_cov_3.806807, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001899 DAOQGJ010000000
VERSION     DAOQGJ010001899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123971_length_1054_cov_3.806807"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PK683_22400"
     CDS             <1..1001
                     /locus_tag="PK683_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNH11267.1"
                     /translation="GITFTGSYIAFAKLNGTMTGKAIMFKGQHLLNLLLALISMIIIG
                     MSVADTKNLNLQIILSVLSLILGVLVVIPIGGADMPVVISLLNSYSGIAGCATGFVLN
                     NYVLVIAGALVGSSGIILTNIMCKAMNRSLLNVILGGFGQTAGGGAEVASSPHGNVKE
                     LGVDEVAMLFDSASSVIIIPGYGMAVAQAQHTVRELMNQLEKKGITVKFAIHPVAGRM
                     PGHMNVLLAEAEVPYDKLVEMDEINSEFSSTDVVLVIGANDIVNPAARTNPASPIYGM
                     PILNADKAKNVIISKRSMAAGYAGIDNELFTYPNAFMFFGDAKASLTKLVNALGEE"
BASE COUNT          294 a          213 c          261 g          286 t
ORIGIN      
        1 gcgggatcac ttttacgggt tcttacattg cctttgccaa actgaacggc acaatgaccg
       61 gaaaagcaat tatgttcaaa ggacagcatc ttttgaacct tctgcttgcg ctgatttcca
      121 tgatcattat aggaatgagt gtcgcagata caaagaacct gaacctgcag attattcttt
      181 ctgttctgtc tctgattctg ggagttcttg tagtaattcc gatcggcggg gcagatatgc
      241 ctgtggtcat ctcacttctg aattcctatt ccggtattgc aggatgtgca actgggtttg
      301 ttctgaacaa ctatgttctc gtcattgcag gtgccctggt aggttcttcc ggaatcatcc
      361 tgacaaacat tatgtgcaag gcaatgaaca gatctcttct gaacgtaatt cttggcgggt
      421 ttggacagac tgcaggcgga ggagcggaag tggcaagttc tcctcatgga aacgtgaaag
      481 aacttggagt ggatgaagtg gcaatgcttt ttgacagtgc aagcagtgta atcatcatac
      541 cgggatacgg tatggcagta gcccaggcgc agcatacggt aagagaactg atgaatcagc
      601 tcgaaaagaa aggcattaca gtcaaatttg caattcaccc ggttgcagga aggatgccag
      661 gccatatgaa cgtacttttg gctgaagcag aagttcccta cgataaactt gtagagatgg
      721 atgaaatcaa cagtgaattc tccagtaccg atgtggttct ggtcataggt gcaaacgata
      781 ttgtaaatcc ggcggcaaga acaaatccgg caagtccaat ctacggaatg ccgatcctga
      841 atgcagacaa ggcaaaaaat gtcatcatca gcaaacgaag catggcagca ggctatgcag
      901 ggattgacaa tgaactcttt acctacccga acgcatttat gttcttcggg gatgcaaaag
      961 cttctttgac caaacttgta aatgcactgg gagaagagta aatatttgtt catgatgaaa
     1021 tatgcagttc tgttttttct tgtctgggtt cagt
//
LOCUS       DAOQGJ010001900         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124053_length_1054_cov_2.445445, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001900 DAOQGJ010000000
VERSION     DAOQGJ010001900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124053_length_1054_cov_2.445445"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PK683_22405"
     CDS             complement(<1..687)
                     /locus_tag="PK683_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH11268.1"
                     /translation="MIKKLKILNMYLFIFCCTLSGLYSDELLFREPEVKPSDAFSMIG
                     DSRTQYIFLPGNPSADWEDEPYLGQYRSACSGGKTGIQNAGIAGSTASDWISILRDGK
                     LKPESLHERVVVLVGGNDVLRHIHKWKGAYSWNITEREEILDAIHSDVKEIVRILRSW
                     NKSVIVQTHFYANPKVKKASTKAVNQGVTGLNLRLYESYYPKDDGKSYDSEVSLAFVK
                     PEFDSKLFFDQ"
     gene            complement(684..>1054)
                     /locus_tag="PK683_22410"
     CDS             complement(684..>1054)
                     /locus_tag="PK683_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11269.1"
                     /translation="LINPKRKEKPSGKLNIIINTAPNDLDWSYFLSLLDSDGTLTFVG
                     VPPSPMELNIGQLLSKRKRITASPIGGRAIMTEMLKIAADHKVFPVIEVFPPSEIEKA
                     MDKVRQNKIRYRAVIDFRKS"
BASE COUNT          268 a          246 c          206 g          334 t
ORIGIN      
        1 ctggtcaaaa aacagttttg aatcaaactc cggctttaca aatgcaagac tgacttcgct
       61 gtcgtagctc ttcccgtcat ccttaggata atatgattca taaagtctca gattcagtcc
      121 cgtcactccc tgattcactg cttttgttga agccttcttc actttaggat ttgcataaaa
      181 atgagtctgc acaatgactg atttgttcca ggatctgagt attcttacga tttccttcac
      241 atcactgtga attgcatcga gaatttcctc tctctctgtg atgttccagg aatatgcacc
      301 cttccacttg tgaatatgcc tgagcacatc gtttccgccc acaagaacga ccaccctttc
      361 atgcagggac tccggtttga gtttgccgtc ccgaagaata gatatccagt cggacgcagt
      421 gcttcctgct atccctgcgt tctgaatgcc tgtctttcct ccggaacagg ctgacctgta
      481 ctgaccgaga taaggttcat cctcccagtc ggctgacgga ttgcccggaa gaaaaatata
      541 ctgagttctg ctgtctccta tcattgagaa tgcatctgag ggtttcactt ccggttccct
      601 gaaaagaagt tcatcggaat acagtccaga cagagtacag cagaatatga ataaatacat
      661 attcaatatt tttaattttt ttatcatgat ttcctgaaat caattactgc acggtacctg
      721 attttattct gtctgacttt atccattgcc ttttcaattt ctgagggagg gaatacttct
      781 atgacaggaa atactttatg atcggccgca atcttaagca tttcagtcat aattgccctt
      841 ccgccgattg ggcttgctgt gattcttttc cttttgctga gaagctgtcc tatattcagc
      901 tccataggtg aaggaggcac tcctacaaaa gtcagtgtgc cgtcactgtc cagaagactg
      961 agaaaataag accagtccag atcgttaggt gctgtgttaa taatgatatt caattttccg
     1021 gaaggtttct cctttctctt aggattgata agaa
//
LOCUS       DAOQGJ010001901         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124200_length_1053_cov_4.017034, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001901 DAOQGJ010000000
VERSION     DAOQGJ010001901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124200_length_1053_cov_4.017034"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PK683_22415"
     CDS             complement(<1..677)
                     /locus_tag="PK683_22415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11270.1"
                     /translation="MKQNPENTASLIQKGNPMAIVINKVHIGTNNGEFFSSRPMCRFW
                     CDTGEVAVILTLDLGNGEDPKDYFVGFYEGVNNESDLPFYDLLAYATASLDASTIRIK
                     LTVIDMDNYSVFDNKFTNYLRELTPQISKTMPAGSAISDVLISVGSYILKEAQKDDKI
                     MELEFQLYDVPKDSKDKNAPVNYNDTTGIPIIEEKNFAVIRTADSREWTGRNSTGDFY
                     FSKTLDLL"
BASE COUNT          272 a          227 c          214 g          340 t
ORIGIN      
        1 agcagatcca gtgtcttgct gaaataaaaa tcacctgttg aatttcttcc tgtccattcc
       61 ctgctgtctg ctgtgcggat tactgcaaag ttcttttctt cgataatcgg tatccctgtt
      121 gtatcattat aatttacagg agcattttta tcttttgaat ccttaggaac atcataaagc
      181 tggaattcca gttccatgat tttgtcgtct ttctgtgctt ctttcagaat ataggaacct
      241 acagagatca gtacatccga tatggcagaa ccggcaggca tagtcttgct gatctgaggt
      301 gtcagttccc tcagataatt tgtaaattta ttatcaaaaa cagaataatt gtccatatca
      361 atgacagtga gtttgattcg gattgtagac gcatccagtg acgctgttgc ataagccaga
      421 aggtcataaa agggcaggtc ggattcattg ttgactcctt cataaaaacc gacaaaataa
      481 tccttcggat cttctccatt tcccagatcc agggtcagaa tgactgccac ttcaccggta
      541 tcgcaccaaa atctgcacat cggtctgctc gaaaagaact ccccgttatt cgttccgata
      601 tgcactttat tgatgacgat cgccatcgga ttgcctttct gaattaggct tgctgtattt
      661 tcgggattct gcttcatgca attttggcat ttctggcagt ccgttgtatc ctgttttttc
      721 ggtttttctg cacattcact gcagccggaa cattcagaat attctgctcc cttcagcctg
      781 ctgtaccgat cttccgttgt gttgtaatat tcggtgcctg cctgtaaatc cttttctttt
      841 aaaagaatat tcagctgacc ggaagttgtt gtacaatgca cggtcagaaa aagaaagcaa
      901 aggatgactg cactgaatgg atttttgagg gtttttttcc tagggaaaaa aagacgactt
      961 gaaaatcttg taaaagcttc ctgaaaatct agtagattat acctagccgc tgcctgcact
     1021 gagcaagcga agcgtgtcga agtgttcgct gtt
//
LOCUS       DAOQGJ010001902         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124257_length_1053_cov_2.680361, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001902 DAOQGJ010000000
VERSION     DAOQGJ010001902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124257_length_1053_cov_2.680361"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..>1053
                     /locus_tag="PK683_22420"
     CDS             64..>1053
                     /locus_tag="PK683_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNH11271.1"
                     /translation="MSKVKWFVPGDIDGLFGLMLDNLIQLLVLVGLCIGVCGFPAEFV
                     FKVILPGVACSLVVGNVFYSWQAAKLAEKEGRSDVTALPYGINTVSMFAFIFFVMMPV
                     YMNTKDYKLAWKVGLLACFLSGLIEVAGSFIAEFIRKHTPRAAMLSALCGIAITFISM
                     DFFIKTFQRPILAFLPLSVILIQYFGRVRLPFGMPAGLASVIIGSAIAWSMGIWSKPM
                     MNPQAVSDSFSFLGFHFPVISILDLFSVMNQESIKEYAAVIIPMGVANVIGSLQNIES
                     AEAAGDRFDTRNSLLVNGIGTVAGSFLGSPFPTTIYIGHPGWKALGARIGYSVL"
BASE COUNT          261 a          206 c          255 g          331 t
ORIGIN      
        1 gagcctgatt caaaagtaat agaagttgat tttacaggaa agaaaaaata actgagggaa
       61 acaatgagta aagtgaagtg gtttgtaccg ggagacatag acggtctctt cggactgatg
      121 ctggacaatc tgattcagct tctggtgctg gtcggtttgt gcataggagt ctgcggcttt
      181 cccgcagaat ttgtatttaa ggtaattctt ccgggagttg cgtgttcact tgttgtgggc
      241 aacgtatttt attcatggca ggccgcaaaa cttgctgaaa aggaaggacg atcggatgta
      301 actgcactgc cttacggaat caatacagta tccatgtttg cattcatctt ctttgtgatg
      361 atgcctgttt atatgaatac caaggactat aaacttgcat ggaaagtagg tcttcttgcc
      421 tgtttcctga gcggtctgat tgaggttgcc ggttctttca ttgccgaatt catcagaaag
      481 catactccga gagcggcaat gctttccgca ctctgcggta ttgcaattac atttatttca
      541 atggattttt tcattaagac atttcagaga ccgattcttg catttcttcc tctctccgtc
      601 atactgattc agtatttcgg aagagtaaga cttcctttcg gaatgcctgc aggtctggct
      661 tcagtcatta tcggttcagc aattgcctgg agtatgggga tctggtcgaa gccgatgatg
      721 aaccctcagg ctgtcagtga ttcattcagt tttctggggt ttcattttcc tgtgatttcc
      781 attctggacc tcttcagtgt aatgaatcag gaaagcatta aggaatatgc tgcagttatt
      841 attccgatgg gtgtggcgaa tgtgatcggc tctctgcaga acattgaatc agcagaagca
      901 gcaggagaca gatttgatac aagaaattct ctgcttgtaa acggaatcgg cacagtagcc
      961 ggatcttttc ttggttcacc ttttcctact acaatttata tcggtcatcc cggctggaag
     1021 gctcttggag caagaatagg gtactctgtt ctg
//
LOCUS       DAOQGJ010001903         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124268_length_1053_cov_2.598196, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001903 DAOQGJ010000000
VERSION     DAOQGJ010001903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124268_length_1053_cov_2.598196"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1053
                     /gene="hflX"
                     /locus_tag="PK683_22425"
     CDS             60..>1053
                     /gene="hflX"
                     /locus_tag="PK683_22425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNH11272.1"
                     /translation="MEKELDLEILDRTSLILEIFAKRAKTREAKLQVESARLKYLLPK
                     LIGSNESLGRQSGGVGTRNRGSGEKKLELDRRKIEEKISELNKELKLLENERKTQKKM
                     RSKTDFPSVALVGYTNAGKSTLMNAMVEFSQKSESKKVFEKDMLFATLETSVRRISLP
                     ENRTILLSDTVGFVNKLPHDLVKAFRSTLDEVRDADLLLHIIDSSHPNFRQQMEVTNE
                     TLCQIGAKTIPTIYVFNKADLTSMKIPSVDGDSVFISAKEKIGITELIQLIIRKAFSR
                     YRECRMLIPYDQGRIVSYLNENAHIKSVSYESNGTLLDLECKEQDYQKYEEFVVQ"
BASE COUNT          355 a          200 c          235 g          263 t
ORIGIN      
        1 agacggaagc gaatatcatt atttttaaca atgaattatc cccttctcaa gtcaagaatt
       61 tggagaaaga actggatctg gaaattctgg acaggacatc tctgattctg gaaatctttg
      121 ccaaaagagc aaaaacaaga gaagcaaagc tgcaggtgga atcagcccgt ctgaagtatc
      181 ttctcccgaa actcattggc tccaatgaat cacttggaag gcagagcgga ggtgtaggca
      241 ctagaaacag aggatccggt gaaaagaaac tggaacttga ccgaaggaaa attgaggaaa
      301 aaatatccga gctgaacaag gaactgaaac tcctcgagaa tgaacggaag acacagaaaa
      361 aaatgagaag caagactgat tttccatctg ttgctctggt aggatatact aatgcaggaa
      421 agtcaacttt gatgaatgca atggtggaat tctctcaaaa atctgaatca aagaaagttt
      481 tcgaaaagga tatgctgttt gccaccctgg aaacttcagt aagaagaatt tcacttccgg
      541 agaacagaac gattcttctt tcggatacag taggatttgt caataagctt cctcatgatt
      601 tagtgaaggc attccgttcc actctggatg aggtcagaga tgcggatctg cttctgcata
      661 tcattgattc ttcacatccg aacttcagac agcagatgga ggtgaccaat gaaacactct
      721 gccagatcgg agccaaaact attccaacca tctatgtatt caacaaggct gacctcactt
      781 caatgaagat tccctctgtg gatggagaca gtgtcttcat ttccgcaaaa gaaaaaatcg
      841 gaatcactga actgattcag ctcatcatcc ggaaagcttt cagcagatac agggaatgca
      901 gaatgctgat tccctatgat caggggagaa tcgtttctta tttgaatgag aatgctcaca
      961 taaaatcggt cagttatgag agtaacggca ctcttctgga tttagaatgt aaggaacagg
     1021 attatcagaa atatgaggaa tttgtagttc agt
//
LOCUS       DAOQGJ010001904         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124299_length_1053_cov_2.314629, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001904 DAOQGJ010000000
VERSION     DAOQGJ010001904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124299_length_1053_cov_2.314629"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            474..>1053
                     /locus_tag="PK683_22430"
     CDS             474..>1053
                     /locus_tag="PK683_22430"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNH11273.1"
                     /translation="MKRLIVSFQNWIYFHLENNPALINKVLFRIFLGAGLTVPSLILV
                     SFWPSASEHSAVPESKPDYKIQEPIQPIPISLSIDFQKAELGEKLFNDVRLSNDNTIS
                     CATCHNLKTGGTDRRKVSVGIKGLEGSINAPTVFNSGFNFRQFWDGRAPNLEAQAEGP
                     VHNPKEMGSSWKEVIEKLKSDEDYKGRFKLVFR"
BASE COUNT          350 a          211 c          186 g          306 t
ORIGIN      
        1 aaacttatta tcaatattat tatgaacagc agcagtttct tcatttcttt ctccattgtt
       61 tgatatgaaa cttcattcgc tttcctgcat ttcaaatcct aaaaaactga cagggaaatc
      121 agatattcct tgtaatgtga aacagctggg gtccaactga aagcattata ttcgattttc
      181 gaaaatttaa aacctgatat tttcaaaaac aagtttctga ataaacttat ctgcaccgaa
      241 tagatcaaac ttctgtaaag ttcatatcat ttccaatccg aaatgagaaa ccaagcaaaa
      301 tgtttatacc atcttatatt tctggaattt aaattcagtc agcttctttt cgaatggaaa
      361 atttttcgga agacccgcaa tccctgaaaa tctgaaacga aattttttcc ggttgtcaga
      421 aaaatacata tattcttata ttctataaat tcctattgca gctttggaga tatatgaaaa
      481 gactcatagt cagttttcaa aactggattt attttcacct ggaaaataat ccggctctca
      541 taaataaagt actgttcaga atttttttag gagcaggtct caccgttccc tcactcatcc
      601 tagtgtcctt ctggccttct gcatcagaac attcagcagt cccggaatca aaacctgatt
      661 acaaaatcca ggaacctatc cagccaatcc caatcagtct cagtatagat tttcaaaaag
      721 cggaactggg agaaaaactt tttaatgatg tcagactgtc gaatgataat acaatatcgt
      781 gtgcaacctg tcataacctg aagactggcg gtactgacag aagaaaagta tcagtcggaa
      841 taaaaggctt ggagggcagc ataaacgccc cgaccgtttt caactctgga tttaatttca
      901 gacagttttg ggacggcagg gcaccgaacc tggaggctca ggcagaagga cctgtccaca
      961 acccaaagga aatgggcagc agctggaaag aagtcattga aaagttgaaa tccgatgagg
     1021 attataaagg cagattcaaa ctggtcttcc gat
//
LOCUS       DAOQGJ010001905         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124493_length_1052_cov_2.493480, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001905 DAOQGJ010000000
VERSION     DAOQGJ010001905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124493_length_1052_cov_2.493480"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PK683_22435"
     CDS             complement(<1..575)
                     /locus_tag="PK683_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11274.1"
                     /translation="MIKSVIFLIIFFVISECKETAKGDLKFGAKMEMLPIDTNNDGKK
                     DAIGYYVNNSENHRIVYQEHDADQNGKSEFFVWSGLSAMDTNKPTKDTVKVHEEEDTD
                     KDGKIDTIRWLLPNDFIAMSQVDRDADGYFETTNYYTMRKTIARSEVDTNKDGYPDQF
                     FWTRRVEVDTDFDKKPDKYYVGSSEADIRTKA"
     gene            complement(575..>1052)
                     /gene="fliN"
                     /locus_tag="PK683_22440"
     CDS             complement(575..>1052)
                     /gene="fliN"
                     /locus_tag="PK683_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNH11275.1"
                     /translation="GSGFSGGDAPSVGGGSGEDDPLAGLGDFGGDSSSMNIPSSDAIA
                     AALGTGGFTPPPPPKTIAKPTPMSGNSSNLNLLMDVTMSLTVELGRTNMLIKDILQLS
                     EGAIVELDKAVGEDLDLLANGKIVGRGKIIVIDDCYGVQVTYIVNPAERFSMAQSL"
BASE COUNT          255 a          267 c          206 g          324 t
ORIGIN      
        1 gcttttgttc tgatatctgc ttcagaactt ccgacataat acttgtccgg ctttttatca
       61 aagtcagtat ccacttccac tcttcttgtc cagaaaaact gatcagggta accgtcctta
      121 ttcgtatcca cttcagaacg ggcgattgtc ttcctcattg tatagtagtt cgtggtttca
      181 aaataaccgt ctgcatctct gtccacctga ctcattgcaa taaaatcatt tggaagaagc
      241 cagcggattg tatcgatctt tccatccttg tctgtatcct cctcctcgtg aacctttact
      301 gtatccttgg tcggtttatt cgtgtccatt gcagaaagtc cgctccagac aaaaaactct
      361 gatttgccgt tctgatcggc atcatgctcc tggtatacaa tccgatgatt ttctgaatta
      421 tttacataat aaccgatggc atccttcttg ccgtcattat tggtatcaat cgggagcatt
      481 tccatctttg cgccaaactt aaggtcacct tttgcagttt ccttgcattc ggagattacg
      541 aagaatatta ttaaaaaaat tactgatttt atcattacag actctgtgcc atactgaatc
      601 tttctgcagg gttgactata tacgttactt gaactccata acagtcatca atgacgataa
      661 ttttccctct cccgacgatt tttccatttg caagaagatc cagatcctct ccgacggcct
      721 tgtccagttc cactatggcc ccttcagaaa gctgaagaat gtccttgata agcatatttg
      781 tccttccgag ttcaacggtc agggacattg ttacatccat cagaagattc agattggagg
      841 aatttccgct cataggagtc ggcttggcga ttgttttcgg ggggggagga ggagtaaatc
      901 cgcctgtgcc cagggcagca gcaatcgcgt ctgaacttgg gatattcata gatgaagagt
      961 cccctccaaa gtctccaaga cctgcaagag gatcatcttc cccgcttcct ccgccgacgg
     1021 acggagcatc ccctccgctg aatcctgacc cg
//
LOCUS       DAOQGJ010001906         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124692_length_1051_cov_2.961847, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001906 DAOQGJ010000000
VERSION     DAOQGJ010001906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124692_length_1051_cov_2.961847"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PK683_22445"
     CDS             complement(<1..384)
                     /locus_tag="PK683_22445"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNH11276.1"
                     /translation="MQIIISKEKISERVKELGKRITSDYQNKSLIVLCTLKGSIIFFA
                     DLIREIELELETEFIRASSYGKEKTSSGDVKLAFPSALSLIGKHVLLVEDIIDTGMTL
                     DTIVNSLYTRNPVSIRVCSLLLKKDA"
     gene            complement(396..905)
                     /locus_tag="PK683_22450"
     CDS             complement(396..905)
                     /locus_tag="PK683_22450"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNH11277.1"
                     /translation="MKQIFLVRHSKSDWDTPFDRDHNRGLSLRGIKNANSLRKVLKLY
                     GIYPEKAFISDARRAVETFKILNRRKELLSNFSITPELYDCNPSQYLEIIKNLNNDIK
                     SVLFLGHNPELEAVANILIGGAKQNLFQKYSTSSLLSLSFERDEWKYVAEQPNGKINF
                     FWIPPKTKT"
BASE COUNT          291 a          211 c          198 g          351 t
ORIGIN      
        1 agcatccttt ttcagaagaa gtgagcatac tcgaatggat actgggttcc tcgtgtacag
       61 agaattcact attgtatcca gagtcatccc ggtatctatg atatcctcca ctagaagaac
      121 atgtttccct atcaggctta aagcggacgg gaatgcaagt ttcacatctc ctgaagaggt
      181 tttctccttt ccgtatgagg atgcacgtat gaattctgtc tcgagctcca gctcaatttc
      241 ccggatgaga tctgcaaaga aaatgatgct tccctttagg gtgcaaagga ctatcagtga
      301 tttattctgg taatcagaag taattctttt tccgagttct tttactcttt cagatatttt
      361 ctctttcgaa attattatct gcatacacag cctcgttagg tttttgtttt tggcgggatc
      421 caaaagaaat tgatttttcc gtttggctgt tctgctacgt atttccattc atcacgttca
      481 aaggaaagtg agagcagtga tgatgtggaa tacttctgga acaggttctg cttagctccg
      541 ccgatcagaa tatttgctac ggcttccagt tcaggattat gtcccaaaaa tagaactgac
      601 ttaatatcat tgtttagatt ttttatgatt tccagatatt gcgacggatt gcagtcgtaa
      661 agttcaggtg taattgaaaa attactcaga agttcctttc ttctgttcag tattttgaaa
      721 gtttccacag cccttcttgc atctgaaatg aatgcttttt ccggatatat tccgtacagt
      781 ttcagaacct tacgaagaga attcgcattt tttattccgc gaagagaaag gcctctgtta
      841 tgatctctgt cgaaaggagt atcccaatcc gatttggaat gacggaccaa aaaaatctgc
      901 ttcatgacct aatgataaat tgccctgaaa aaaatccctt gcttttttat tattctatca
      961 aatcttttac tgagagagtc aatatggacg aaatccttaa actagtaaag tcattctaca
     1021 ttcctcggga aataccagcc taccttatca a
//
LOCUS       DAOQGJ010001907         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124710_length_1051_cov_2.652610, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001907 DAOQGJ010000000
VERSION     DAOQGJ010001907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124710_length_1051_cov_2.652610"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..>1051)
                     /locus_tag="PK683_22455"
     CDS             complement(59..>1051)
                     /locus_tag="PK683_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH11278.1"
                     /translation="IGKGGKEVWLQATYSPIFDLNGKPFKAVKYATDITAQKKLNLEM
                     EKIIRDMVHSLGAMEKGDLTQSLRNEYGAGFNQLKTSLNNTLNKLKSILMEVMNSADM
                     LLSASEQVSITAQSLSSSSSEQAASVEETSASLEEMSAAISQNAENTKKTDQTAARTS
                     MNAVQGGQAVRDTIKAMRQIAEKISIIEDIAYQTNLLALNAAIEAARAGEHGKGFAVV
                     ASEVRKLAERSQFSANEVRDLAVSSVDTAEKTGSLIAEIVLSVNGTAELVREISISSS
                     EQSSGAEQISKAVAQLDSVTQQNASSSEELASTAEELNSQAERLQQIVKFFRIT"
BASE COUNT          229 a          272 c          207 g          343 t
ORIGIN      
        1 ataaaaagta tacttaccag tgctgtttca atatgttcca ttactgaatt ctgtcccctc
       61 aggttattcg gaaaaacttc acaatctgct gcagtctttc tgcctgactg ttgagttctt
      121 ctgcggtgga tgcgagttct tcagaagaag atgcattctg ctgagtgaca gaatccagct
      181 gtgccactgc cttgctgatc tgttcagccc cggaactctg ttcactgctg gatatactga
      241 tttctctcac tagctctgct gtcccattga cagaaagaac aatctctgca atcagactgc
      301 ctgttttttc agctgtatcc acactgctca cggccagatc acggacttca tttgccgaaa
      361 actgactccg ttcggcaagc ttacggactt ctgaagcgac taccgcaaat ccctttccgt
      421 gttcacctgc acgggcagcc tctattgcgg cattcagtgc cagcagattt gtctgatatg
      481 caatatcttc aatgatgctg atcttttctg ctatctgtct cattgctttt atggtgtcac
      541 ggacagcctg tcctccctgc acagcattca ttgatgtgcg ggctgcagtc tgatctgtct
      601 tctttgtgtt ttctgcattc tgagaaattg cggcactcat ttcctccaga gatgcactgg
      661 tttcttcaac acttgcggcc tgttcacttg aagaactgct gagggactga gccgtaatgc
      721 tgacctgctc ggaagcactc agaagcatat ctgcactgtt catgacttcc atcagtatac
      781 tttttaattt atttaatgta ttatttaatg atgtcttcag ctgattaaac cctgctccat
      841 attcattccg cagggactga gtcagatctc ctttttccat cgcgccgagc gagtgaacca
      901 tatcccgaat aattttttcc atttccaagt tcagcttctt ctgtgcagta atatctgtcg
      961 catattttac tgccttgaaa ggttttccgt tcagatcaaa gatcggactg taggttgcct
     1021 gaagccatac ttccttcccg ccctttccta t
//
LOCUS       DAOQGJ010001908         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124781_length_1051_cov_1.986948, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001908 DAOQGJ010000000
VERSION     DAOQGJ010001908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124781_length_1051_cov_1.986948"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PK683_22460"
     CDS             <1..771
                     /locus_tag="PK683_22460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH11279.1"
                     /translation="YLRATFESMGDAVITTDQFGKIRRMNFVAEKLTGWKFSEAEGKT
                     VQKVYQTDSFLSENPGFNLYDRLNDSSSDPTKTEHTHLISRNGKKLHVTDTVSPIRDS
                     KGRIIGAVIVFRDMTEDCRIKLELIESEERFRSVVEWFPQPIVVHKDCKILYANPATA
                     RMFHTDSPSDIIGHSIFEKQDSSFHEISIDRVSEHLETGKDLPVIEQRFTRFDGTVFA
                     AEVQGTSIVYDGNPAILSCMWDISERKRSNDFCPQGLH"
BASE COUNT          325 a          199 c          229 g          298 t
ORIGIN      
        1 tatctcaggg cgacatttga atcaatggga gatgctgtaa ttacgactga tcagttcgga
       61 aaaatcagaa gaatgaattt tgttgctgaa aaactgaccg gctggaagtt ttcagaagcg
      121 gaagggaaga ctgttcagaa agtttatcag actgattctt ttctctctga aaatccaggg
      181 ttcaatctgt atgaccggct gaatgattca agcagtgatc cgactaagac agagcatact
      241 catctgattt caagaaacgg aaaaaaactt catgtcactg atactgtttc ccctatccgt
      301 gattcaaaag gaagaatcat cggtgctgtt attgtattca gggatatgac ggaggactgc
      361 aggatcaagc tggagctcat tgagagtgaa gaaagattcc gcagcgtagt tgaatggttt
      421 cctcagccga ttgtagtgca taaggactgt aagattcttt atgcaaatcc tgccactgcc
      481 agaatgttcc atactgattc tccttcagat atcatcgggc acagtatctt tgaaaaacag
      541 gacagcagtt ttcacgagat ttcaatagac cgggtttcag agcatctgga aacggggaag
      601 gatcttcctg tcattgagca gagattcacc cgttttgacg gaacagtctt tgctgcagag
      661 gttcagggaa cttcaattgt ctacgacgga aatcctgcaa ttctcagctg tatgtgggac
      721 atttccgaaa gaaaaagaag taatgatttc tgcccgcaag gtctgcattg aaaacgaaga
      781 ttatatttta gcacattatc tttgcacaga ttaatatttt ttctcacgga cttctttttc
      841 aggtgatgaa actgtcggcg gtctgtgtta tcggcagaac tgtttcataa ttcagaaaat
      901 caataagaaa cttatttaca aaataacctg tccgatttcc aatcctatta tgaaaaagcc
      961 gacagacgga acgaatcaaa ttttttcgaa tataaaatcg gaaaaaataa gggcaaagac
     1021 agcactcttt ttcatactga ctgcgcttct g
//
LOCUS       DAOQGJ010001909         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124926_length_1050_cov_2.527638, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001909 DAOQGJ010000000
VERSION     DAOQGJ010001909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124926_length_1050_cov_2.527638"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PK683_22465"
     CDS             complement(<1..357)
                     /locus_tag="PK683_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11280.1"
                     /translation="MKLLSVLFLSLFFGSLHSAPKIEKIDISAYPMIRVFIFEDYRRR
                     LEEEKIIIQERIDEEVYSISSAEILRYRNPRPVNVLFSIMSSTWEKNKSSKEAAMMIS
                     SLLKDEDRIYLHFFSGE"
     gene            complement(370..>1050)
                     /locus_tag="PK683_22470"
     CDS             complement(370..>1050)
                     /locus_tag="PK683_22470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH11281.1"
                     /translation="ILDEISFSLEPGRIHVLAGASGSGKTTAALTVFGIIPKNFILSF
                     SDYKILEKDYSEKKELERLYGKKIVLIPQNISSCFHPYFSLSSQIRDYLEGAVSDSEI
                     LGILKSAGIRNPEEKMKRNPISLSGGERQRILLKTALHSLPELIVADEPSSALDPVFE
                     EEILEELVGAVKNRNSSLLLISHNARIIRKYADSVSVFNRGKILETAEREKHGFTLKT
                     EYAKNLLL"
BASE COUNT          290 a          227 c          212 g          321 t
ORIGIN      
        1 ttccccggaa aaaaaatgga gataaatcct gtcttcatcc ttcagaagag aggatatcat
       61 cattgcggct tccttggatg acttattctt ttcccaggtg gaggacatta tggaaaaaag
      121 gacatttaca ggtcttggat tcctgtaacg gagtatctct gcagaggaaa tactgtacac
      181 ttcctcatca attctttcct gaataatgat tttttcctct tccagtcggc gtctgtagtc
      241 ctcaaatata aagacccgta tcatcggata tgcagaaata tcaatttttt caattttggg
      301 agctgaatgc aggctcccga aaaaaagaga cagaaatagt accgacagta acttcatcta
      361 cagctaaatt tataaaagca gattttttgc atactctgtt ttgagagtaa atccgtgttt
      421 ctctctctct gcagtttcca gaattttccc cctgttgaac acagaaaccg aatcggcata
      481 cttgcggata atgcgtgcat tgtgagaaat cagcagaaga gaagagtttc tgtttttgac
      541 cgccccgacc agctcttcga ggatttcttc ttcaaatacc ggatcaagtg cagaactggg
      601 ttcatctgcc acaatcagct ccggtaaaga atgcagtgct gtcttcagaa ggattctctg
      661 tctttctccg cctgagaggg aaatcgggtt ccttttcatt ttttcttcag gatttctgat
      721 gcctgcactt ttcagaatgc caagaatttc agaatcggat accgcccctt ccagatagtc
      781 acggatctgg gacgacagtg aaaaatacgg gtgaaaacag gaagagatat tctgaggaat
      841 cagaacaatt tttttaccgt aaagcctttc cagttctttc ttttcagaat agtctttttc
      901 cagaatttta taatcagaaa agcttagtat aaagtttttg ggaataattc cgaaaactgt
      961 cagtgccgcc gttgtctttc cgcttccgga tgcgcctgcc agcacatgga ttcttcccgg
     1021 ttccaggctg aatgaaattt cgtccagtat
//
LOCUS       DAOQGJ010001910         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_124954_length_1050_cov_2.306533, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001910 DAOQGJ010000000
VERSION     DAOQGJ010001910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124954_length_1050_cov_2.306533"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PK683_22475"
     CDS             complement(<1..416)
                     /locus_tag="PK683_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11282.1"
                     /translation="MKKLSFIFMILCFSSCMTFTRFEETEGFCDWYFGRAGLEGTVRH
                     HSGPERNGYGLLHFRLPELIINTQHGLYYPDGEDDEKFLLEAGIVEWIFPYREFASSK
                     ISSQDKMTFFLFNPFYTAPKRIFGLLIRLPVYLIHDV"
     gene            complement(418..>1050)
                     /locus_tag="PK683_22480"
     CDS             complement(418..>1050)
                     /locus_tag="PK683_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11283.1"
                     /translation="TDCTKCQSKYCNCSNTKSGGKNSSDNRSSSNQNQKQNISNNPSF
                     VNEIHIHSSGSAQRSAEGDSIKLSVGTSLFGWNESKLTEEGKVQLKGFMQFLDRHSNM
                     NLAIIGQVSPEGEASLNNNLAEARVYAVKNYLKDYSDRIGFIYSCGKEFSCLNCWKKQ
                     GPKDKNPPPDECCRRVNFIFYEKEKEADIREELKKAHPEKFCTNHSDRKK"
BASE COUNT          274 a          218 c          209 g          349 t
ORIGIN      
        1 acatcgtgaa tcaggtatac agggagacga atcaaaagtc cgaagatcct tttgggagct
       61 gtataaaaag gattgaacag aaaaaatgtc attttatcct gagaggatat tttgctgctt
      121 gcaaattctc tgtacgggaa tatccattca acgatccctg cttccaaaag aaacttttca
      181 tcatcctcac catcagggta gtatagaccg tgttgagtat tgatgatgag ttccggcaga
      241 cggaaatgca gaagtccgta tccgtttctt tctggcccgc tgtgatgcct gactgttcct
      301 tccagacctg ctcttccaaa ataccaatca cagaaacctt ctgtttcctc gaatcttgtg
      361 aaagtcatgc aggatgagaa acaaaggatc atgaaaataa atgataattt tttcatatca
      421 ttttttcctg tcactgtggt tggtacagaa tttttccgga tgggcttttt tcagttcttc
      481 acggatatca gcttcttttt ccttttcata aaaaatgaag tttactcttc tgcagcattc
      541 atccggcggc ggatttttat ctttcggccc ctgttttttc cagcagttca gacaggagaa
      601 ttcttttccg caggaataga taaatccgat cctgtcggaa taatctttca gataattttt
      661 cactgcatag actctggctt ctgccaaatt attgttcagt gaagcttcgc cttcaggact
      721 tacttggccg atgatggcaa gattcatatt tgaatgcctg tccaaaaact gcataaatcc
      781 cttcagttgg acttttcctt cctcagtcag tttagattca ttccatccaa acagagatgt
      841 tcctacgctc aatttgatgc tgtctccttc tgctgatctc tgtgcagaac cggaactgtg
      901 gatatgaatt tcattgacaa aactgggatt gttggaaatg ttctgcttct gattttggtt
      961 tgaagaggaa cggttgtctg aagaattttt cccgccagat tttgtatttg aacaattgca
     1021 gtatttggac tgacatttcg tgcaatcggt
//
LOCUS       DAOQGJ010001911         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125104_length_1049_cov_3.122736, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001911 DAOQGJ010000000
VERSION     DAOQGJ010001911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125104_length_1049_cov_3.122736"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..>1049
                     /locus_tag="PK683_22485"
     CDS             93..>1049
                     /locus_tag="PK683_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002105680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH11284.1"
                     /translation="MSGILGEYTELFLEESEDQIEELNGNLLKLESDHSNPEIINDIF
                     RAAHSLKSSAAFVGLYNLSDLAHKMENLLQKIREDKISVNVTLVNLLFECFDLIKSVI
                     DAVGQGEKIDTPFTDMIEKLGNYEKSSSTSSAAVQVKKTSPAVSERPAEKKQESSNGI
                     LNFIDLNAEEISILNEEISVRNASAIDVKITLKPEVQMKGSRFALILQNLKNFGVIFK
                     SLPEEDELLSGGQFTELVFTFITQLDLVELEKIISSDQIQDVKFSLRTNDLGNSGEFH
                     PLNLTQEELQELDEEMKNRKGQAYDVRVILKSNTQMKGLRFTL"
BASE COUNT          342 a          189 c          223 g          295 t
ORIGIN      
        1 tatgattgac tttgtaggaa gaagcggcga ggatatcgtg cttcttatag atttgaaaaa
       61 actgattaaa atcgattgag cgggagcggg tcttgtctgg aatccttgga gaatatacag
      121 aactttttct ggaagaatca gaagatcaga ttgaggaatt aaacggcaat cttctgaagc
      181 tggagtccga tcattcgaat ccggaaatca taaatgatat tttcagagcc gcgcattctc
      241 tgaaaagttc agctgccttt gtcggattat acaatctttc cgatctggcg cataaaatgg
      301 aaaatcttct tcagaaaatt agagaagata aaatctcagt aaatgttact ttagtcaatc
      361 ttctctttga atgtttcgat ctgattaaat cagtcatcga tgcggtcggc caaggtgaga
      421 aaatagatac accttttacc gatatgattg aaaaactcgg taattatgaa aaaagttctt
      481 ctacttcatc tgcagctgtt caggtaaaga agacttctcc tgcggtttct gaaagaccag
      541 cagaaaaaaa acaggaatct tccaacggaa ttctgaactt tattgacctg aatgcggaag
      601 aaatttccat tttgaatgaa gagatatctg taagaaacgc aagtgccatt gatgtcaaaa
      661 tcactttgaa gcctgaagtg caaatgaagg gttccagatt tgcacttatt cttcagaatc
      721 tgaaaaactt tggagtcatt ttcaaatcac ttcccgaaga ggacgaactt ctttccggag
      781 gacagttcac agagctggtg tttacattta tcactcagct tgatctggtt gaactggaaa
      841 aaattatatc ctccgatcag atacaggatg taaagttttc actgagaaca aatgacttgg
      901 ggaattcagg cgaattccat cccctgaacc tgactcagga agaacttcag gaacttgatg
      961 aggaaatgaa gaacagaaaa ggccaggctt atgatgtcag agtgattctc aaaagcaaca
     1021 ctcagatgaa aggcctgcgt tttaccctg
//
LOCUS       DAOQGJ010001912         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125155_length_1049_cov_2.397384, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001912 DAOQGJ010000000
VERSION     DAOQGJ010001912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125155_length_1049_cov_2.397384"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PK683_22490"
     CDS             <1..789
                     /locus_tag="PK683_22490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH11285.1"
                     /translation="QDVISLCDKTLRNSERAESVFEKRKIVIFGEPNAGKSSLLNLLL
                     GKERAIVSPIPGTTRDFLSEDMDLRGIPLRLVDTAGIRDTEDQIEKAGIEKSRKEFDS
                     ADIRVAVFDTSVPFNEKEIQKNYNKMHEGTVFVLNKSDIMHKSWNADRLKSALGIAES
                     AFISVSCTQRTGIEELLEEIHERMHERKMDPDFVFLEDRNRFFFRMISESVKTALELF
                     NTGAPSEIYVQDINRALSAIGKIQGKADTEEILGRIFSKFCVGK"
     gene            812..>1049
                     /locus_tag="PK683_22495"
     CDS             812..>1049
                     /locus_tag="PK683_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNH11286.1"
                     /translation="MEQFSILNLGLGGGLRILGSGFVFGTKLIRILQDMFLDNKNPDY
                     PVLLREAFEELGATYIKLGQFIASAPSLFPKEYTQ"
BASE COUNT          334 a          176 c          251 g          288 t
ORIGIN      
        1 caggatgtca tatctctctg cgataagact ctcaggaatt ctgagagagc tgagtccgta
       61 tttgaaaaaa gaaagattgt aattttcgga gagccgaatg caggaaagtc cagtcttctg
      121 aatcttctcc tgggaaagga aagagcgata gtatctccca ttccgggaac aactagagat
      181 tttctttcag aggatatgga tcttcggggg attcctctcc gtctcgtaga tactgcggga
      241 ataagagata ctgaggatca gattgaaaaa gctggaatag aaaaaagcag aaaagaattt
      301 gattcggcag acatccgtgt ggctgtattc gatacctcgg ttccttttaa tgaaaaagag
      361 atacagaaaa attataataa aatgcatgaa ggaactgttt ttgtgctgaa caaatccgac
      421 attatgcata aaagctggaa tgcggacaga ctgaagagtg ctctgggaat tgctgaatca
      481 gcattcattt ccgtgtcctg cacacagaga actggaattg aagaacttct cgaagagatt
      541 cacgaaagaa tgcatgagag aaaaatggat cctgatttcg tttttctgga agacaggaac
      601 agattttttt tcagaatgat ttcagagtcg gtaaaaacgg cactggagct tttcaatacg
      661 ggagcacctt cggaaattta tgttcaggat atcaacaggg cgctttcagc aataggaaaa
      721 atacagggaa aggctgatac agaagaaatc cttgggagga ttttcagcaa attctgtgta
      781 ggaaaataat catcgggaga ttaactagag tatggaacag ttttcaattc tgaatcttgg
      841 acttggaggc ggactgagaa ttctcggttc aggctttgtg ttcggaacca aacttattcg
      901 gattcttcag gatatgtttc tggataataa gaatcctgat tatccagttc ttctaagaga
      961 agcatttgaa gaactgggag cgacttatat taaactggga cagttcattg catctgctcc
     1021 gtctcttttt ccgaaagagt atactcagg
//
LOCUS       DAOQGJ010001913         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125158_length_1049_cov_2.373239, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001913 DAOQGJ010000000
VERSION     DAOQGJ010001913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125158_length_1049_cov_2.373239"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PK683_22500"
     CDS             complement(<1..641)
                     /locus_tag="PK683_22500"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNH11287.1"
                     /translation="MTVDEVLAELKKMGNESTKKVLMKHGAKEPFFGVKVQDLKKIVK
                     KVKKDYELSLQLFETGNSDAMYLAGLIADEKKMKKKDLENWAKKAYWYMLSEYTVAWV
                     TAESPFGMELGLEWIDSDKENISSAGWATLGAIATYTKNEDLDLKLFEKLILRAEKDI
                     KTAPNRTKYAINTFIISVGSYAAPLKEKCEKTAEKIGKIQVEMGGTECKVPNAP"
     gene            698..>1049
                     /locus_tag="PK683_22505"
     CDS             698..>1049
                     /locus_tag="PK683_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNH11288.1"
                     /translation="MNRFSLAYSPCPNDTFLFYHLVHENLSSQFSIREELHDVEHLNQ
                     AAEKNVYDITKLSFFAFFHFSDRYVLLNSGSALGRGCGPILVKKKGRKVPDLENCRIA
                     VPGLLTTANLLQSSE"
BASE COUNT          264 a          251 c          183 g          351 t
ORIGIN      
        1 ggagcattag gcaccttaca ttcagtaccg cccatttcca cctgtatttt tccgattttt
       61 tctgctgttt tttcacactt ttccttcaat ggggccgcat atgaacccac gctgataata
      121 aatgtattta ttgcatactt tgtcctgttc ggtgcagttt ttatatcttt ttccgcacgc
      181 aaaatcagtt tttcaaaaag cttcagatcc agatcctcat tctttgtgta cgtcgcaatt
      241 gctccaagag tcgcccagcc tgcagaggaa atattttcct tgtctgaatc aatccattcc
      301 agaccaagtt ccattccaaa cgggctttcc gcagtgaccc aggcaacagt atattcggaa
      361 agcatatacc agtatgcttt ttttgcccag ttttcaagat cttttttttt catttttttt
      421 tcatcggcaa tgagtcccgc cagatacatg gcgtccgaat ttcctgtttc aaaaagctga
      481 agcgagagtt catagtcctt ttttactttc ttcactatct tcttgagatc ctgaactttc
      541 accccaaaga aaggttcttt tgctccgtgt ttcatcagaa ctttctttgt gctttcattt
      601 cccattttct tcagttctgc cagaacttca tctacagtca taacgattga ttcaaatcac
      661 ttgaccggat tcgtcaagta aaaatgctga agcaagaatg aacagattca gtttagctta
      721 ctccccctgc ccaaatgata cattcctttt ttatcatctt gttcatgaaa atctttcatc
      781 ccaattctcc atccgggaag aactgcatga tgtagaacac ttgaatcagg cggctgaaaa
      841 aaatgtatat gatattacga aactctcctt ttttgcattt tttcattttt cggacaggta
      901 tgttctccta aactccggtt cggcactcgg cagaggatgc gggcctatac tggtaaaaaa
      961 gaagggcaga aaggttccgg atctggaaaa ctgcagaatc gctgttcccg gccttctgac
     1021 gactgcaaat cttctgcagt cgtcagaag
//
LOCUS       DAOQGJ010001914         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125296_length_1048_cov_3.712991, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001914 DAOQGJ010000000
VERSION     DAOQGJ010001914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125296_length_1048_cov_3.712991"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /gene="trmD"
                     /locus_tag="PK683_22510"
     CDS             <1..158
                     /gene="trmD"
                     /locus_tag="PK683_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569698.1"
                     /note="methylates guanosine-37 in various tRNAs; uses
                     S-adenosyl-L-methionine to transfer methyl group to tRNA;
                     Chlamydial proteins show a C-terminal extension of unknown
                     function; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNH11289.1"
                     /translation="KDESHNIEGVLEYPQYTKPSIYHGWEVPEVLLNGNHAEIKKWNE
                     SHRKNSQ"
     gene            233..670
                     /gene="rplS"
                     /locus_tag="PK683_22515"
     CDS             233..670
                     /gene="rplS"
                     /locus_tag="PK683_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNH11290.1"
                     /translation="MDHAIEKVFEEDYKRRTLNFGIGDTVKVHYRILESGKERIQVYE
                     GTVISLANKMNDKSFTVRRISYDVGVERIFPLYSPRVAKIELVRKGRVRRAKLFYLRK
                     RTGKTARIKELRGGQAIVQAEKKREKEAAKAEAAVKTEAPAQA"
     gene            689..>1048
                     /locus_tag="PK683_22520"
     CDS             689..>1048
                     /locus_tag="PK683_22520"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11291.1"
                     /translation="MHHTKFEDIESLYKDFLVFGIDEAGRGPVAGPLSVACLSFETDF
                     LHSKEFSEKFKDLNDSKKLSEKKRNSLYALLTEDKNVIYGHIFVSNLFIDRFGIAEAI
                     FQGIRKLTQKFSDRQSFY"
BASE COUNT          370 a          177 c          226 g          275 t
ORIGIN      
        1 tgaaggatga atctcataat attgagggag tgttggagta tcctcagtat acaaaaccgt
       61 ccatctatca tggatgggaa gtgcctgaag tgcttctgaa cgggaatcat gccgagataa
      121 aaaaatggaa tgagagccat agaaaaaact ctcagtaagt tcagatttta tctgaataat
      181 atactgatat acactgattg cctcgatggc tgaacagtaa aaggacaaaa aaatggatca
      241 cgcgatcgaa aaagtattcg aagaagacta taaaagaaga acattgaatt ttggaattgg
      301 ggacacagtg aaagttcatt acagaattct tgaatctgga aaagaaagaa ttcaggtcta
      361 tgaaggaact gtgatttcat tggcaaacaa aatgaatgac aagtctttca cagtcagaag
      421 aatttcttat gatgttggtg tagaaagaat tttccctctt tattctccta gagttgcaaa
      481 gatcgaactg gtcagaaaag gtagggtcag aagagcaaaa cttttctatc tcagaaagag
      541 aacaggtaaa actgccagaa tcaaagaact cagaggcgga caggctatcg ttcaggctga
      601 aaagaaaaga gagaaagaag ctgccaaagc agaagcagct gttaagacag aagcacctgc
      661 tcaagcttaa acttcccaat caaggaagtt gcaccatact aaatttgaag acattgaaag
      721 tctctataag gactttcttg tctttggaat tgatgaggca ggaagaggtc ctgtagcggg
      781 gcctttatcc gttgcctgtc tctcctttga aaccgatttt ctgcattcaa aggaattctc
      841 cgaaaaattc aaagacttaa acgattccaa gaagctctcg gaaaagaaga gaaactcttt
      901 atatgcactt ctgactgaag ataagaatgt aatatacggt catatctttg tatcaaacct
      961 gttcattgat cggtttggaa ttgcagaagc catttttcag ggaatcagaa agctcactca
     1021 gaaattttct gatcgtcagt ccttttat
//
LOCUS       DAOQGJ010001915         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125383_length_1048_cov_2.498489, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001915 DAOQGJ010000000
VERSION     DAOQGJ010001915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125383_length_1048_cov_2.498489"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..>1048)
                     /locus_tag="PK683_22525"
     CDS             complement(214..>1048)
                     /locus_tag="PK683_22525"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH11292.1"
                     /translation="KDLESLKAGSEKEITAFFSDIAGFSNISEKLSPKDLSLFLNEYL
                     SAMTEILKQNKGTLDKYIGDAIVGIFGAPVHSENHYLTAAYASLEMLQALEQIKARWK
                     EENRFFESVWNMKIRIGINTGIAKVGFMGTEKIASYTMIGDSVNLAARLESAAKDYGV
                     SILISEFTKERIDKEIFTRLLDRIIVKGKTEPISVFEMVGFPAEISSQNIESAKLYES
                     AFLLYQERKWDLALKRLKESQRVRQKPDKAAELLSERCRAYKLKPPSKDWDGVFKREN
                     K"
BASE COUNT          304 a          208 c          200 g          336 t
ORIGIN      
        1 gagaaacaga ttctttctga taatgacagt ataattgaat tgggagaaaa actggtaaaa
       61 aaaatccaag tggatctgaa aaatgataag cagtcgcatt ttatcaacgt atataattgc
      121 aatgtaacag aaacggagag aaccgaaaag aaagaatcta cagaaggcct gtagtttttt
      181 aatgtgggtt cgggtcatat cgaattcacg ttttcattta ttttctctct tgaatactcc
      241 gtcccagtct ttggacggag gtttcagttt gtatgccctg catctttcag agaggagttc
      301 tgccgctttg tccggtttct gccgcactct ctgagattct ttcagacgtt tcagtgccaa
      361 atcccatttt ctttcctgat aaagaagaaa ggcggactca tacagtttgg cggactcaat
      421 gttctgagaa gagatttctg ctggaaaacc gaccatctca aaaacggaaa tcggttctgt
      481 tttgcctttt acgataatcc ggtccaaaag tcgggtaaaa atttccttat caattctttc
      541 ttttgtaaat tcactgatca aaatactgac accgtaatcc tttgcagctg actccagtcg
      601 tgcggcaaga ttaacagaat ccccaatcat ggtgtatgaa gcaatttttt ccgtgcccat
      661 aaatccgact ttagcgatac ctgtgtttat gccgattctg attttcatat tccagacaga
      721 ttcaaaaaat ctgttttctt ctttccatcg cgcctttatc tgttccaatg cctgaagcat
      781 ttctagagat gcataggcgg cagtgagata atgattttct gaatgtaccg gtgccccgaa
      841 aattccgaca atggcatcac cgatatattt atcaagagtg cctttgttct gttttaatat
      901 ttcagtcata gcagaaaggt attcatttaa aaacaaagaa agatcttttg gagaaagttt
      961 ctcactgatg ttggaaaaac ctgctatatc ggaaaaaaag gctgtaattt ctttttctga
     1021 tcctgctttc agactttcaa gatccttc
//
LOCUS       DAOQGJ010001916         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125421_length_1048_cov_2.196375, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001916 DAOQGJ010000000
VERSION     DAOQGJ010001916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125421_length_1048_cov_2.196375"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..753)
                     /locus_tag="PK683_22530"
     CDS             complement(43..753)
                     /locus_tag="PK683_22530"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenesis-related family 1 protein"
                     /protein_id="HNH11293.1"
                     /translation="MKYTAILLILLPILLLSYEKSKVDQAYNMLSRSWSYSENGTSVN
                     VKSLKKSCKSADCRLIVNGKDIDPAAVLGETPSGSETVPAEKEVSAAANGSEEGRMKG
                     ITEAHNKYRKSKNLPELVWDKNIASYAQEWANHLKNSRGCKMQHRSADEYGENLAWSM
                     GRELNSADPVKMWHDEEKDYNYQSNKCNPGAVCGHYTQVVWKNSKKLGCGAAVCGNSE
                     VWVCNYYPPGNYVGEKPY"
     gene            complement(814..1014)
                     /locus_tag="PK683_22535"
     CDS             complement(814..1014)
                     /locus_tag="PK683_22535"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH11294.1"
                     /translation="MGKKKFLYDIWGDTVNLASRMETGSAPGKINISESVYEKIKGEF
                     KCQFRGRLPVKGKGRIGMYFVE"
BASE COUNT          260 a          253 c          195 g          340 t
ORIGIN      
        1 cagacgatat cggtctgaaa aagtgccagt cttaatgatc cctcagtatg gtttttcgcc
       61 gacataattt cccggaggat aataattgca gacccagacc tcggaattcc cgcatactgc
      121 tgccccgcat cccagttttt tggagttttt ccagaccacc tgagtataat gaccgcatac
      181 tgctccggga ttacatttgt tgctctgata attataatct ttttcttcat catgccacat
      241 cttcacggga tcggcagaat tgagctccct tcccatggac caggcaagat tttctccata
      301 ttcatctgca gacctgtgct gcatcttgca tcctctgctg ttcttcagat gatttgccca
      361 ttcctgcgcg tacgatgcaa tatttttgtc ccagacaagt tcaggaaggt tctttgattt
      421 tctgtattta ttgtgcgctt ctgtaattcc tttcatcctt ccctcttcag atccgtttgc
      481 ggctgccgac acttcctttt ctgcaggaac agtttcagag cctgaaggag tctcgcccag
      541 gaccgctgcc ggatcaatgt cctttccgtt cacaatcagt ctgcagtcag cggatttgca
      601 ggattttttg agtgacttga cattgacaga agttccgttt tcagaatagc tccatgaccg
      661 ggaaagcata ttatatgcct ggtctacttt tgatttctca tatgaaagga gaagaatagg
      721 aagcagaatc agaagaatgg ctgtatattt catacagagc aatttactct gaactgaaaa
      781 atttcaactc atttttaaac tgcatataaa aatttattca acaaagtaca tccctattct
      841 tccctttcct ttcactggga ggcgtcctct gaattgacat ttaaactcac cctttatttt
      901 ctcatacacg gattcactga tgtttatttt acccggagca ctgcctgttt ccattcggct
      961 ggccagattg acagtatcac cccagatatc atacagaaat tttttctttc caatgacgtt
     1021 gaatacgtaa tggggaacaa tcagttat
//
LOCUS       DAOQGJ010001917         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125441_length_1048_cov_2.045317, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001917 DAOQGJ010000000
VERSION     DAOQGJ010001917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125441_length_1048_cov_2.045317"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..861
                     /locus_tag="PK683_22540"
     CDS             427..861
                     /locus_tag="PK683_22540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11295.1"
                     /translation="MTFLLLVFSHCTEKKEDSSKLLAAAVLASRSGSASSTSSTGSTT
                     GSSGLTPSSTFSCSVASPAFSTLASAGTTSNCSKSGCHNTASSQQGLDITSYTSVKAK
                     AVSGDPEGSLIYQKITSGTMAVNSNSAIAQAVYCWIKGGLNP"
BASE COUNT          324 a          201 c          227 g          296 t
ORIGIN      
        1 agtcctgctt tgaactgaac gggcgggatg aagaagcaga tagactgcaa tgatgatgct
       61 gtatatcagg acaggaaggc ggccgagtga attggctgaa aagtaaatat gttcaataaa
      121 attcatatgg atcagcaaat aaattcgttt actgtaagca gaatgaaaag taaaaaattc
      181 gatggatgca gatttatacg gactttcctg actgcataat tagcaaggag gcgacgagag
      241 tctcagtatg agcctttctc ggaaatatat ttttgactta aatgctcaat gcttcagaat
      301 gacggttgat tttgaaatga agtctgagaa agaactcaaa ttaacggacc tctttcagcc
      361 ttcattaaag attaaacagg atcgtttaaa ggattttcaa atactgtgaa aacttatatt
      421 tcatatttga catttttatt gctggtcttc agtcattgca ctgagaaaaa agaggacagt
      481 tcaaaacttc tcgcagcagc agttcttgct tcccgctcag gatctgcctc ttccacatcg
      541 agcactggaa gtacgaccgg atcatccgga ttgactccat ccagcacatt cagctgttca
      601 gtggctagtc ctgcattttc aactcttgca tcagcaggga cgacgtcaaa ctgttctaaa
      661 agcggatgtc ataatacagc cagctcacag cagggactgg acattacaag ttatacttcg
      721 gtgaaagcaa aggcagtatc cggggatccg gaaggaagtc ttatttatca gaaaatcact
      781 tcaggaacaa tggcagtgaa ttcaaattct gcaatagcac aggcagtata ctgctggatc
      841 aaagggggac tgaatccttg aatgcaaaat tttattgaat taaagaggca ggaaatccgg
      901 cctctgaaca gaggcacgga tacaataaat tacttgacag tcagttctga tttgactttg
      961 tctacagttg cttttccaaa gcctgggaca tcatccagat cttccacttt tttgaatttt
     1021 ccgtttttct ttctgtggtc gataattg
//
LOCUS       DAOQGJ010001918         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125771_length_1046_cov_4.282543, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001918 DAOQGJ010000000
VERSION     DAOQGJ010001918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125771_length_1046_cov_4.282543"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1046)
                     /locus_tag="PK683_22545"
     CDS             complement(<1..>1046)
                     /locus_tag="PK683_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11296.1"
                     /translation="EYDYENPDPETAPEAAAENQGDPQVNMEQLDYPKSAVTDEQGNY
                     KISKLPKGSYNLTFYREGYIVETASIKKVESGKSFRLAMIMTRPSSISGKVLIKDVLP
                     ETGAKNIMITLNSPVKTYNAYSYHDGTFQIQDIKPGKYALSFTHRGFHDIRIKDLEIR
                     EGQVLTGQNKIIQAKDPSLTLYSSNYTYPAGNKVSFNLRSYRIDKVDLKIYQVPASHL
                     VLPPAKPEKIDPDKFGYKVVQKWEEPIPRYQPYDWMYTDISPKEPLPLGTYCFEASYT
                     DNIIERKFFTVTSTGIIAKRSESGFLIYATDLITNKPLENASVYVFLNETLLQEKKAK
                     MAVPFLAAAESRAA"
BASE COUNT          249 a          213 c          241 g          343 t
ORIGIN      
        1 cttgcggccc tgctttctgc ggctgctaag aacggcacgg ccatctttgc ttttttttcc
       61 tgaagcagtg tttcattcag aaatacatat acggatgcat tctccaaagg tttgtttgta
      121 ataagatcag ttgcatatat caggaatcct gattcagatc tttttgcaat gatgccggta
      181 gatgtgactg tgaagaattt tctttcaatg atattgtctg tataggatgc ctcaaaacag
      241 tatgttccca gaggaagagg ttctttcggg gatatgtcag tgtacatcca gtcgtaaggc
      301 tggtatcttg gaatcggttc ttcccatttc tgcacaacct tgtatccgaa tttatcagga
      361 tcaatttttt ccggcttggc gggagggagc acaagatgag atgcaggcac ctgataaatt
      421 ttgagatcca ctttgtctat tcggtaggac cgcagattga aactgacctt gttcccggca
      481 ggatatgtat aattggagga atacagtgta agagaaggat cctttgcctg tatgattttg
      541 ttctgtccgg tgagaacctg tccttccctg atttccagat ccttgattct gatatcgtga
      601 aatcctctgt gagtaaaact cagagcatac tttccaggtt tgatatcctg aatctgaaag
      661 gttccgtcgt ggtaactgta tgcattgtaa gtctttacag gcgaattcag tgtaatcatt
      721 atattctttg caccagtctc cggcagaaca tccttgatca ggacctttcc ggagatagag
      781 gatggtcttg tcataatcat tgccagacgg aaggattttc cgctttccac ttttttaatg
      841 ctggcagtct ccacgatata gccttctctg taaaaggtca gattgtatga acctttagga
      901 agtttggata ttttataatt tccctgctcg tcagtcacag cggatttcgg atagtccagc
      961 tgttccatat tgacctgagg gtcgccctga ttttcagcgg cagcttcggg agcagtttca
     1021 ggatcagggt tttcatagtc gtattc
//
LOCUS       DAOQGJ010001919         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125785_length_1046_cov_3.439960, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001919 DAOQGJ010000000
VERSION     DAOQGJ010001919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125785_length_1046_cov_3.439960"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK683_22550"
     CDS             complement(<1..283)
                     /locus_tag="PK683_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HNH11297.1"
                     /translation="MDVIPGHVAIIMDGNGRWATAKDLPRSKGHEAGADAIDRLMETS
                     LEIGLKNVSLYAFSTENWKRPASEITSIFGLLDRFIEERLEKVHSRGIRI"
     gene            complement(296..811)
                     /gene="frr"
                     /locus_tag="PK683_22555"
     CDS             complement(296..811)
                     /gene="frr"
                     /locus_tag="PK683_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNH11298.1"
                     /translation="MDKTIEALKKDFAQIRTGRANPAMVEDLKVEYYGAMTSLNQLGT
                     ITTPEPRMIVINPYDKASIKAIEKAILSSGMGFSPSNDGVIIRIPLPELTGERRKELT
                     KLVKAKAEEKKVAIRNIRRDINDDIKKDNSESSQDQIKGLTDQIQKVTDSYIAKIVEI
                     AEQKEKEINTV"
     gene            complement(837..>1046)
                     /gene="pyrH"
                     /locus_tag="PK683_22560"
     CDS             complement(837..>1046)
                     /gene="pyrH"
                     /locus_tag="PK683_22560"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766681.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNH11299.1"
                     /translation="KRYKQISFSESIRRRLKVMDQTALSLCMENNMPIIVFDIFQKGN
                     LQRIINGETVGTLISNSEEVVLDSE"
BASE COUNT          232 a          236 c          193 g          385 t
ORIGIN      
        1 gtattctgat ccctctgctg tggacttttt ccagacgttc ttcaatgaat ctgtccagaa
       61 gcccgaaaat ggaagtgatt tctgaggcag gacgtttcca gttttctgtg gaaaatgcgt
      121 agagggaaac gtttttcagt ccgatttcca gacttgtttc catcagacga tcaatcgcgt
      181 ctgctccagc ctcatgccct ttgcttctgg gaagatcctt ggcagtggcc catcttccgt
      241 ttccatccat aataatggcc acatgtccag ggataacatc catattcaga ctgaatcaga
      301 ccgtattgat ttctttttct ttctgttcag cgatttcaac tatctttgca atgtaagaat
      361 cagtcacctt ctgaatctga tcagtcagtc ctttgatctg gtcctgtgag ctttctgaat
      421 tgtctttttt tatgtcgtca ttaatatccc ttctgatatt tctgatagcc actttttttt
      481 cttcagcttt tgctttgacc agcttagtca gttcctttct tctttctcct gtcagttccg
      541 gaagaggaat tcggatgatt accccgtcat tacttggaga aaaacccatt cctgaactta
      601 agattgcctt ttcgatagct ttaatggatg ctttgtcgta tggattgatt acgatcattc
      661 ttggttcagg agtcgtaatt gtaccaagct gattcaatga agtcattgca ccgtagtact
      721 cgactttcag atcctctacc atagcaggat ttgctcttcc tgttctgatc tgagcgaagt
      781 cctttttcag ggcttcgatt gttttatcca tttttgtctt tgtctgtccg atgtagtcac
      841 tcactgtcta aaaccacctc ttcgctgttt gaaattaatg tccctactgt ttcaccattg
      901 ataattctct gcagattgcc tttctggaaa atatcgaata cgataatcgg catattgttt
      961 tccatacaga gactgagtgc tgtctgatcc ataactttca gtcttcttct gattgactct
     1021 gaaaaagaaa tctgtttgta tctttt
//
LOCUS       DAOQGJ010001920         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125794_length_1046_cov_3.250252, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001920 DAOQGJ010000000
VERSION     DAOQGJ010001920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125794_length_1046_cov_3.250252"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK683_22565"
     CDS             complement(<1..192)
                     /locus_tag="PK683_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002723693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNH11300.1"
                     /translation="MFDFSRYKYISIAISVIAIVLGFAVTIFVHKGFAHSLDFNGGLR
                     AVISLPKEKDRSVIQKFFEE"
     gene            complement(185..>1046)
                     /gene="secD"
                     /locus_tag="PK683_22570"
     CDS             complement(185..>1046)
                     /gene="secD"
                     /locus_tag="PK683_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNH11301.1"
                     /translation="SGNDLTDAKPNLDPNTYSWIVSFTLTSAGAEKFYDVTAKNKGRN
                     LAIIWGDKVVSNPQINDPIAGGHAQITGSFNQEESVRLSNIISEGALPIPLNVLEMRF
                     IGPTLGIESIEVGFKSVFIGFILVMGYMIMYYRLAGLVADITLIANVTLLMALLTLMD
                     FTLTLPGFAGVILTVGMAVDANVIIYERIKEELIEGKPLGNAISNGFENAFSTIFDSN
                     ITTLISGILMVKLGNGPIKGFAITLCWGIITSMFTSLVFSRILLNILADRIGIETLQL
                     GFKKYEAKNV"
BASE COUNT          319 a          230 c          208 g          289 t
ORIGIN      
        1 ctcctcaaaa aacttctgga tgacgcttct gtccttttcc ttcggaagac tgatgactgc
       61 tctgagtcct ccgttgaaat caagtgaatg cgcaaaacct ttatgaacaa aaatcgttac
      121 tgcaaatcct agaacgattg caatgactga aatcgcgatt gaaatgtatt tataacgtga
      181 aaaatcaaac attttttgcc tcgtattttt taaagccaag ctgaagagtt tctattccga
      241 ttctgtctgc aagtatattc agcagaatac ggctgaatac aagtgatgta aacatggaag
      301 taatgattcc ccagcagaga gtgatcgcaa accctttgat aggaccgttg ccaagtttca
      361 ccataagaat tccggaaatc agcgttgtaa tgttactgtc aaagatcgtg gaaaaagcgt
      421 tttcaaaacc gttgctgatc gcattgccga gaggctttcc ttcaatcagt tcttccttga
      481 tcctttcata aataatcacg tttgcatcca ctgccatacc gactgtcaga atgaccccgg
      541 caaatccggg cagagtcaga gtaaaatcca tcaaagtgag aagagccata agaagtgtta
      601 cgtttgcaat aagggtaatg tctgcaacaa gtcctgcgag acggtaatac ataatcatat
      661 atcccatcac aagaataaac cctatgaata cggatttgaa accgacttca atggactcga
      721 taccgagagt cggtccaatg aacctcattt ccagaacatt cagcggtata gggagagcac
      781 cttcactgat aatattggag agacggactg attcttcctg attgaaactt ccagtaatct
      841 gagcatgtcc gcctgcaatc ggatcattga tctgaggatt ggaaaccacc ttgtcacccc
      901 agatgattgc cagatttctt cccttgtttt ttgcagtcac atcgtaaaat ttctcagcac
      961 ctgcagatgt cagtgtaaac gatacaatcc aggaataagt gttcggatca agattcggtt
     1021 tagcatcagt aagatcattt ccgctg
//
LOCUS       DAOQGJ010001921         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125795_length_1046_cov_3.217962, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001921 DAOQGJ010000000
VERSION     DAOQGJ010001921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125795_length_1046_cov_3.217962"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..722
                     /locus_tag="PK683_22575"
     CDS             168..722
                     /locus_tag="PK683_22575"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNH11302.1"
                     /translation="MDERDYFTEDELKKILSYDEWKSHEHRLWLQLMYCFGLTVQELT
                     NLKVKDIDFDSMVLKVYSSRRSKPRILNIPKVIQLDLRKEALGKYSEDHLFQGRKGAV
                     HKRTVQKVMERVFKKFGIYATVSKFRKTFALHSIRYGWDEKSIAMALGHSSIRATRLL
                     MGKDRIYFERKKSHPIDHILRIKL"
     gene            complement(759..>1046)
                     /locus_tag="PK683_22580"
     CDS             complement(759..>1046)
                     /locus_tag="PK683_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LipL21"
                     /protein_id="HNH11303.1"
                     /translation="SGAALTAKADFMRKMIGETLTGAEGVSDGESTGKVVVAEFNGKV
                     KGINTRECRPLKQVQPEAPGIEWEECECVMFARVPGGKDAIVAKAQEVEKK"
BASE COUNT          320 a          192 c          214 g          320 t
ORIGIN      
        1 cctattcata cctttttctg tgataataga aaataggtgt ttcaggtatt tgcttaaata
       61 tgttgaaaat attgtcatat atgtaatatg tgaattatct acttgaaagg cttttaaatt
      121 ttttataagg gtcttttggg tggataataa cttttaagga tgaaattttg gacgagcggg
      181 actattttac agaggacgaa ctgaaaaaaa tactgagtta cgatgaatgg aaaagccatg
      241 aacatagact ttggcttcag ctgatgtact gctttggact gactgttcag gagctgacca
      301 atttgaaagt gaaggacatt gattttgact caatggtatt gaaggtatac agcagcagga
      361 gaagcaagcc taggatactg aatattccga aagtgattca gttggatctc agaaaggagg
      421 cattggggaa atattcagaa gaccatttgt ttcagggaag aaagggagca gttcataaaa
      481 ggactgtaca aaaggttatg gaaagagtct tcaaaaaatt tggaatctat gcaactgtaa
      541 gtaaattccg aaagacattt gcactgcatt caatcagata cggctgggac gaaaaaagta
      601 tagctatggc cctaggtcat tcttccatca gagcaacaag acttttgatg ggtaaagaca
      661 gaatatactt tgagcggaaa aaatcccacc caatagatca tatcctgaga ataaaactgt
      721 aaaaaatgcc cgtttatgac gggcattttc tcgagtattt attttttctc tacttcctgt
      781 gcttttgcta caatcgcatc ttttccgcct ggaactcttg cgaacattac gcattcgcat
      841 tcttcccatt cgatgcccgg agcttcaggt tgaacttgtt tcagtggacg gcactctcta
      901 gtgttgatcc cttttacttt tccattgaat tcagctacaa ccactttacc agtggattca
      961 ccatccgata caccttctgc accagtcaga gtttcaccta tcatttttct catgaaatcc
     1021 gctttagcag tcagtgcagc accact
//
LOCUS       DAOQGJ010001922         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125803_length_1046_cov_3.139253, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001922 DAOQGJ010000000
VERSION     DAOQGJ010001922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125803_length_1046_cov_3.139253"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PK683_22585"
     CDS             <1..466
                     /locus_tag="PK683_22585"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH11304.1"
                     /translation="KVLELGTGIGYSASWMFSSNVPFHLTSADRNAEALEIARNFLKR
                     TAVKDQSFDLERIHILEKMRNIKTLTEYDLIFIDCDKICYPELLSICIEKCRPGASVV
                     FDNALWHGRVFPEIHTKPSDRAVQEFWAKTSEIKANKTLIPAGDGLLLLELL"
     gene            463..>1046
                     /locus_tag="PK683_22590"
     CDS             463..>1046
                     /locus_tag="PK683_22590"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNH11305.1"
                     /translation="MNILDLSALIGYFIIIFAAGLYFGKKGGKDDYYLGGKNLHWNFL
                     MLSIIATETSSLTFLGVPGMSYKGGIQFLQTALGFLFGRIIVAYVFLPLYLKSDISSV
                     YEWIGVMFGKHVQRFSSTLFLFTRLLGDGIRLYATSIPCALLLGNWVQGYSETEVFTM
                     SLAAITAGTMIYTVYGGFKSVVATDSIQFLVYIAG"
BASE COUNT          279 a          228 c          234 g          305 t
ORIGIN      
        1 gaaggttctc gaactgggaa caggaatcgg gtattctgca tcctggatgt tctcctccaa
       61 tgttccgttt catctgactt ccgcagacag aaatgcagag gctctggaaa ttgcccgaaa
      121 ctttctgaag agaactgcag tcaaggatca gagtttcgat ctcgaaagaa ttcatattct
      181 ggaaaagatg agaaacataa aaactcttac agaatacgat cttattttca ttgactgcga
      241 caaaatctgc tatccggagc ttctttccat ctgcattgaa aaatgcagac ccggtgcatc
      301 tgttgtattc gacaatgctc tctggcacgg aagggttttt ccggagatcc atacaaaacc
      361 ctcagacagg gctgtgcagg aattctgggc caaaacatct gaaatcaagg ccaataaaac
      421 actgatccct gccggtgacg gacttttact tctggagctc ttatgaatat tctggatctt
      481 tccgctctca tcggatattt tatcattatt tttgcagcag gactgtattt cggaaagaaa
      541 gggggcaaag acgattatta tctgggaggg aaaaacctgc actggaattt cctgatgctt
      601 tccattattg caacagagac ttccagtctg acatttctgg gcgttccggg aatgagctac
      661 aagggcggaa tacagtttct tcagactgcg ctcggtttcc ttttcggaag aatcattgtt
      721 gcgtacgtat tccttcctct gtatctcaaa tcggatattt cttcagtata tgaatggata
      781 ggagtcatgt tcggaaaaca tgtacagaga ttttcgagca cactgtttct ttttacaaga
      841 cttctgggcg acggaatcag gctctatgca acatccatcc cctgtgcact tcttctggga
      901 aactgggtgc agggatactc tgaaacagag gtatttacaa tgtcgctcgc cgcaatcact
      961 gcaggcacaa tgatttatac tgtttacggc ggattcaagt ctgtagtcgc cactgattca
     1021 attcagtttc tggtctatat tgcagg
//
LOCUS       DAOQGJ010001923         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125823_length_1046_cov_2.795156, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001923 DAOQGJ010000000
VERSION     DAOQGJ010001923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125823_length_1046_cov_2.795156"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..>1046
                     /locus_tag="PK683_22595"
     CDS             110..>1046
                     /locus_tag="PK683_22595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH11306.1"
                     /translation="MGLLLFLSAFPVLFLFEIRLGSPIQYDFSSLETILDSMPINIFI
                     KDMEGKFIFVNAHSEKTLGKSRNEILGKTDYHFFPKETADEIVQYDREAVKKKGETVS
                     RTEELFPGRFYLAGKRLVTLPDEGQGILGFSIDISQTVKAEEELVHQKILFTNLMNTN
                     PSLIFVKDIQGRFLLLNKAVEELFEKPAEEILFRNNYEIHDFVPEVDAYTRIDRQVIE
                     TGEVITVVESHTSVSGKKRWFRTIKAPIAMNDGSMNVLGVSTDITEIVQKEEELIRAK
                     DAAEKSARIKSEFLSTMSHEIRTPLNAVVGITNILL"
BASE COUNT          326 a          196 c          240 g          284 t
ORIGIN      
        1 gtttcatttt cggagaaaag gcatctcagc actcatcagt tttctctgtt ttgcagtaat
       61 ttcagctgcc ggtgaaaccg gacagcttcc tgtgctgttt tctgcagata tgggacttct
      121 tctttttctg agtgcatttc ctgtgctgtt tctttttgaa atccggctgg gcagtcctat
      181 tcagtatgat ttttcctctc tcgaaaccat attggactct atgccgatca atattttcat
      241 caaggatatg gaagggaaat tcatatttgt aaatgcacat tcagaaaaga cgcttgggaa
      301 atcaagaaac gagatattgg gcaagaccga ttatcatttt tttccaaaag agacagcgga
      361 tgaaattgtg cagtacgaca gagaggcagt gaaaaaaaag ggagagactg tcagcagaac
      421 agaagagctt tttccgggaa gattttatct cgcagggaaa aggctagtca ctcttcccga
      481 tgaagggcag gggattctgg gcttttccat tgacatatcc cagactgtca aggcagaaga
      541 agagctggtc catcagaaaa tactttttac gaatctgatg aacacaaatc ccagtctgat
      601 ctttgtgaaa gacatccagg gcagattcct tcttctgaac aaggcagtag aggaactttt
      661 cgaaaaacct gcagaagaga ttttattcag aaataattat gaaatacacg attttgtacc
      721 ggaagtcgat gcctatacaa gaatagaccg tcaggtcatt gaaaccgggg aagtgattac
      781 agtagtggaa tctcatactt ctgtgagcgg aaaaaagcgc tggttcagga caatcaaggc
      841 accgattgca atgaatgacg gaagcatgaa tgtgcttgga gtgagcacag acattactga
      901 aatcgttcag aaggaagaag aactgattcg tgcaaaggat gcagctgaaa aatctgcgag
      961 aataaaatct gaatttcttt ccactatgag tcatgaaatc agaacgccac tgaatgcagt
     1021 cgtaggaata acaaatatcc ttcttt
//
LOCUS       DAOQGJ010001924         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125834_length_1046_cov_2.715439, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001924 DAOQGJ010000000
VERSION     DAOQGJ010001924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125834_length_1046_cov_2.715439"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PK683_22600"
     CDS             complement(<1..885)
                     /locus_tag="PK683_22600"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNH11307.1"
                     /translation="MNIILRLIILLTSIPFLHKCTTIQKNGIARIYAQEINDGSSVNH
                     LKKVKMLPVQNNGKCGFIDENGRMLIEPKYQECSYGENFTTVNTGKKQLIVLFDGTET
                     PFSNIDPFYDGIAVAKKADNDYDVLVDENLNIVSKLDYEYLARLSGKNVYAYEIGGKY
                     SLMDSSEDSLTPEQFDSIFIPAYDEDANIFRVKRDDMYSFIKENGKLFPEFYQFAYDF
                     SHDFALVRISENNYQFINRKGKPAFKDKNFFYAESFQNGFARIFLESGKNCGFLKTDG
                     NYALEPADNSFGCKMIGDK"
BASE COUNT          283 a          196 c          189 g          378 t
ORIGIN      
        1 tttatctccg atcattttgc agccaaagga attatctgca ggttccagtg cataatttcc
       61 gtcagttttt aaaaatccgc aattctttcc tgattcaaga aatattctgg caaagccgtt
      121 ctgaaagctt tcagcataaa agaagttttt atccttgaat gcaggtttac ccttcctgtt
      181 aatgaactga taattatttt cagatatcct taccaatgcg aaatcgtgcg aaaaatcata
      241 agcgaactga taaaattctg gaaacagctt tccgttttcc ttgatgaaag aatacatatc
      301 atctcttttt actcggaaga tattggcgtc ttcatcgtag gcaggaatga agatgctgtc
      361 aaactgttca ggagtgagac tgtcttcgct ggaatccatc aggctgtatt tccctccaat
      421 ttcataggca tatacatttt ttccggacag ccttgccaga tattcataat caagttttga
      481 aactatgttc agattttcgt cgaccagtac atcataatca ttgtcggctt ttttagctac
      541 agctattccg tcataaaatg gatcaatatt tgagaatgga gtctctgttc cgtcaaaaag
      601 tacaatcagc tgtttttttc cagtattgac ggttgtaaaa ttttctccat agctacattc
      661 ctgatacttt ggttcaatga gcattctgcc gttttcatca atgaaaccgc attttccatt
      721 gttctgtaca ggaagcattt ttactttttt cagatggttt acagaagaac cgtcattgat
      781 ttcctgtgcg taaatgcgtg cgatgccgtt tttctgaatg gtcgtgcatt tgtgcagaaa
      841 ggggatagat gtcagcagaa taattaatct gagtattata ttcatttcct tggctagagt
      901 tagattattt ttctaggtat ttgtcggatt tgggatttgc tgtagatctg tctgtttcct
      961 tctaaataaa gaaaccagtg ttttttttat catttccaaa gattctttga attcctttct
     1021 tcgaagcgat acatctattg caatcg
//
LOCUS       DAOQGJ010001925         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125841_length_1046_cov_2.653885, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001925 DAOQGJ010000000
VERSION     DAOQGJ010001925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125841_length_1046_cov_2.653885"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..400)
                     /locus_tag="PK683_22605"
     CDS             complement(182..400)
                     /locus_tag="PK683_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11308.1"
                     /translation="MDRVKKIVLDRHDTSKHFTMLIWFLRILQPLLTGISMHGTPINA
                     MGEWAFHVRLIDARGKNSVKQIRFVSGL"
     gene            complement(622..>1046)
                     /locus_tag="PK683_22610"
     CDS             complement(622..>1046)
                     /locus_tag="PK683_22610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11309.1"
                     /translation="RCLIGQVYRPAENDCRGTGTKATLWGALEFQWCPTNDRSCEKDG
                     VADPALSPAAKACAESNFLGKKWGLGSKTDSLSLYLSNTQETLKLDFSTINELRWITI
                     YFWIKESNSSDAAFYSGTSENKTVSKSTALNVLCKGII"
BASE COUNT          285 a          209 c          203 g          349 t
ORIGIN      
        1 tttcttcttg aaaaggcagg attttggtgc agaactgaaa gctgacactg ctgcgagaag
       61 cagaaccgcc ttttttttct catcgtcttt cttcttactg ctgtcattgc agctgagaat
      121 gaaaatgccg agtatcgctg atgcaagtat tttattcatt atcttgttct ccttaaaaat
      181 atcacagtcc tgaaacaaat cggatctgct tgacagaatt ctttcctctc gcatcaatga
      241 gccggacatg aaatgcccat tcacccattg cattgatcgg agttccgtgc attgagatgc
      301 cagtcagcaa tggttgtaat attcgtaaga accagatcag cattgtgaaa tgttttgaag
      361 tatcatgtct gtccaaaacg atttttttga ctctgtccat tgcaagtttc ttattgtttt
      421 ctttgcaggc atctgcatca ggaaaatctg ctgacgggca agtgtagggt gcttcgcttt
      481 tatattgttt ttgtatattt gtggaatcat tctttttagt aggatcttca ggaatacaag
      541 ataatgatac ttgatcacct ggaaacgaat ataatgatgc acagcataga attaagagaa
      601 gtagcaatcg ataaacattc attatattat ccctttacaa agcacattaa gagcagttga
      661 tttgctgaca gttttatttt cgctagttcc tgaataaaag gcagcatcac tagaatttga
      721 ttcctttatc caaaaataaa tagttatcca gcgaagttca ttgattgtag agaaatccag
      781 ttttagtgtt tcttgagtat ttgatagata caatgacaaa ctgtctgttt tacttcctaa
      841 tccccatttt ttcccgagga aatttgattc cgcacatgcc tttgctgcag ggcttaaggc
      901 gggatcagcc acaccgtcct tttcacagga acggtcattg gtaggacacc attgaaattc
      961 caatgctccc cagagtgtag cttttgttcc agtgcctctg cagtcatttt ctgcaggtct
     1021 gtatacctga ccgatgagac atctct
//
LOCUS       DAOQGJ010001926         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_125998_length_1045_cov_4.192929, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001926 DAOQGJ010000000
VERSION     DAOQGJ010001926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125998_length_1045_cov_4.192929"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PK683_22615"
     CDS             complement(<1..339)
                     /locus_tag="PK683_22615"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH11310.1"
                     /translation="MPHPLLKLIETASETLLGKRREIELAAAAWCAGGHILIEDIPGV
                     GKTTLALLLAKLAGFEISRIQFTSDLLPADILGVSIYHHDKKEFEFRKGPIFSPVVLA
                     DELNRASPKTQ"
     gene            596..958
                     /locus_tag="PK683_22620"
     CDS             596..958
                     /locus_tag="PK683_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11311.1"
                     /translation="MKKVAVWLFLAVLISVPLSAKDRDRKVKKNSDSVKGEPAETVRP
                     EGNQFDSFGQSMQNSMAEMVRMMYRTVFTEYSKKENTDLIASYYKNLYDSLIEKGFTK
                     EEAMKIILNAGVPSVSGK"
BASE COUNT          295 a          202 c          265 g          283 t
ORIGIN      
        1 ctgggttttc ggagatgctc tgttcagttc atcagcaagt acgacgggtg aaaagatggg
       61 accctttctg aattcaaatt cttttttatc atgatgatat atagatactc ccagaatatc
      121 cgcagggagc agatcgcttg tgaactgaat tctggatatt tcaaaaccgg ccagtttagc
      181 cagaagaagt gcaagagtgg ttttccctac acctggaata tcttcaatca gtatgtggcc
      241 gcctgcgcac caggccgctg cagcaagttc aatttcgcgg cgtttcccca gaagggtttc
      301 ggaagcagtc tctatcagtt tcagaagggg atgaggcata tctttatatg atctgctgag
      361 agcgtttttt gtccagcaaa gcttggtcgt taatctgctt gactgcctgg cggatttttg
      421 aataatcagt ctgaatgctc aagcagcata taatgttctg gaatcataat ctgccctgca
      481 gagaaaggga acctgatttg aaaagtgagt tcaaccccgg tcagcactga agcaggattg
      541 ctgtaagcag tgcagagcaa gtcgtgcggc tgaactttca aataaggaga atataatgaa
      601 aaaagttgcg gtatggcttt ttctggctgt gctgatctcg gttcctctct ctgcaaagga
      661 cagggacagg aaggttaaga agaacagcga cagtgtgaag ggagaaccgg cagagacagt
      721 ccgcccggaa ggaaatcagt ttgattcatt cggtcaaagt atgcagaaca gtatggctga
      781 aatggtgcgt atgatgtaca ggacagtgtt tactgagtat tccaaaaagg aaaacacgga
      841 tctgattgca tcttattata agaatctata tgattctctg attgaaaaag gatttacaaa
      901 agaagaggcg atgaagatta ttctgaatgc cggtgttccc agtgtttcgg gaaaataagt
      961 cggcatatct gctgacgggg actgaactcc gtcagcagat ccattctgaa tgccgtgctt
     1021 acttcaatgc agcaattgca ttttc
//
LOCUS       DAOQGJ010001927         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126026_length_1045_cov_3.029293, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001927 DAOQGJ010000000
VERSION     DAOQGJ010001927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126026_length_1045_cov_3.029293"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PK683_22625"
     CDS             <1..305
                     /locus_tag="PK683_22625"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH11312.1"
                     /translation="YTYNEADGKPLLALLHGAGGNTSHFPSILRRNTEFSSLAIDLPG
                     HGKSEGTALNSIDLYTEFLNEFILQKNLKNFFLVGHSMGGLISQNYILKYDHSAAS"
     gene            complement(673..>1045)
                     /locus_tag="PK683_22630"
     CDS             complement(673..>1045)
                     /locus_tag="PK683_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11313.1"
                     /translation="ILYVLIYLFFIMPAAVQGKDEERSELSGRIFVGKPVKEVSEPGC
                     EGCGEPGYIKFEDSRRFEMAWPGSDEIESGTYTRKGLKIYIRIGDAGKAHLFVLSKDG
                     IQLVCREMNFFLRDKRYPWKK"
BASE COUNT          286 a          253 c          188 g          318 t
ORIGIN      
        1 attacactta caatgaggcg gacggaaagc cgcttcttgc actgcttcac ggggccggcg
       61 gcaatacctc tcattttcct tccatcctca gaagaaacac tgaattcagc tcgcttgcga
      121 ttgatcttcc cggtcatgga aaatcggaag gaactgcact gaattcgatt gatctctata
      181 ctgaatttct gaatgaattc atactccaga aaaatctgaa gaattttttc ctggtaggtc
      241 attcgatggg gggactgatt tctcagaatt atatattaaa atacgatcat tccgctgctt
      301 catagtttgg gaaaccgccg gtaggcctgc caaaaacgcc ccacctgcag ccgtaaggcg
      361 taaagtgaga tcctaaatct ccttacgatg atcagcatcc ggtgattctg cagagaaaag
      421 aaaacatgga aaagctgaat tcaacagtcg ctgaattcag tgaaatcttt actcttctgc
      481 ggggttatac cgttagaaca ttatttttaa atataaattc attatactgg aaatattctt
      541 cttccgtcag aaaacatatc cttcatccga aaatgggcgg aagaattttt cagccctatg
      601 aatttgctga acttgctgtg tattcaggtt tcagactggg agactttttc aggattgtcc
      661 ataattactg cttcattttt tccatggata tcttttatcc ctcagaaaaa aattcatctc
      721 cctgcagaca agctgaatcc cgtccttgga cagcacaaac agatgagctt ttcccgcatc
      781 ccctatccgt atgtaaatct tcagaccttt tcttgtatat gtcccgcttt ctatttcatc
      841 acttcccggc caggccattt cgaaacgacg gctgtcttca aatttgatgt accccggttc
      901 tccgcatccc tcacatcctg gttctgacac ttcctttaca ggctttccta caaaaattct
      961 gccgctcagc tctgatctct cttcgtcctt gccttgaaca gcagccggca taataaaaaa
     1021 tagatatatt aatacatata atatt
//
LOCUS       DAOQGJ010001928         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126027_length_1045_cov_3.014141, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001928 DAOQGJ010000000
VERSION     DAOQGJ010001928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126027_length_1045_cov_3.014141"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1045)
                     /locus_tag="PK683_22635"
     CDS             complement(<1..>1045)
                     /locus_tag="PK683_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11314.1"
                     /translation="NGISLYLNPPECRPASPAKKSGNCFFAVLNPAVREGDGLKIMIA
                     APEYSGQVLRISLQSSGPRYFSKESWFINLPIGSGGIYIGEVFPDRIPGPVTVWLEIP
                     GSGEVCRTVYFTGAKSLYTFDVKEIQSNYDSFEVSGNLIFRGKKPAADSVRAIAYCSR
                     CGSPVGTGNFPVKEDSVKMKFAVDDHIRSHKHDYALHLFYGESQCISVMNIFEENAQE
                     AAFEELPERTCGEEIKFDLKADEGEMLFYHISDTKEEDRRTRQILQRGNLALSGAADK
                     NFNRKASPLEISFSAGELAMLSDPMQGPEIRMVWSYDPERGHIYKPYKWENISDLYIS
                     FYRRTEAGRIQPMA"
BASE COUNT          285 a          251 c          196 g          313 t
ORIGIN      
        1 agccataggc tgaattcgtc ctgcttcagt ccttctgtaa aaacttatat aaagatctga
       61 aatattttcc catttatagg gcttatatat atgacctctt tccgggtcat aactccatac
      121 cattctgatt tcaggcccct gcatcggatc tgaaagcatt gcaagctctc ctgcactgaa
      181 tgaaatttcc agaggcgaag cttttcgatt gaaattttta tcagcagccc cggataaagc
      241 aagatttcct ctctgcagaa tctgccttgt tcttctgtcc tcttccttgg tatctgaaat
      301 atggtaaaag agcatttctc cttcatctgc cttcagatca aatttaattt cctctccgca
      361 ggtgcgttcc ggaagttctt caaatgctgc ctcctgagca ttttcttcaa aaatattcat
      421 aacggatata cactgacttt ctccataaaa cagatggaga gcataatcgt gcttatgcga
      481 gcggatatga tcatccactg cgaatttcat tttcactgaa tcttctttta cagggaaatt
      541 tccggtacct acaggagatc cgcaccgcga acagtaggct attgccctga cagaatcagc
      601 agcaggtttt ttccctctga aaatcagatt tccggaaact tcaaaagaat cataattact
      661 ttgtatttct tttacatcaa atgtatacag agatttcgct cctgtaaaat acactgttct
      721 gcacacttca ccggatcccg ggatttccag ccagacagtg acggggcccg gaattctgtc
      781 cgggaaaacc tccccaatat atattccgcc agacccaatc ggcagattga tgaaccagga
      841 ttctttggaa aaatatcttg ggccggagga ctgaaggcta atccggagca cctgtcctga
      901 atattcaggt gcagcaatca taattttcag tccgtcacct tctcgaactg caggattcag
      961 gacagcaaag aaacagttcc cgcttttctt tgcaggactt gcaggtctgc attctggagg
     1021 atttaagtat aatgaaattc catta
//
LOCUS       DAOQGJ010001929         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126038_length_1045_cov_2.865657, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001929 DAOQGJ010000000
VERSION     DAOQGJ010001929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126038_length_1045_cov_2.865657"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PK683_22640"
     CDS             <1..309
                     /locus_tag="PK683_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein YsxC"
                     /protein_id="HNH11315.1"
                     /translation="EMVNGYLNHTETLKILFILLDSAREIPEEEKDLIQVCLEKNIQP
                     VLIRTKIDKRNQKETAVLKKEKQILALEFPELKVIFASSKNGRGLEEIRNILSESLYG
                     "
     gene            302..571
                     /locus_tag="PK683_22645"
     CDS             302..571
                     /locus_tag="PK683_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH11316.1"
                     /translation="MDKNEVLLSLRKIPGIGKSISEDLWNIGIRGIEDLKNKNPYDLY
                     EKSNEYAGTVQDRCLLYVFRTAVYFAETPENSREPEKLKWWNWKD"
     gene            579..>1045
                     /locus_tag="PK683_22650"
     CDS             579..>1045
                     /locus_tag="PK683_22650"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNH11317.1"
                     /translation="MLQAKIYSAFGFLLGDMFGSPFESRSSEEAAKGFGHKVGSYTDD
                     TLMYLSVMKAFRYDLPLSETLIEFYDGKRGYGSRMDEMLSKKICAPTDSWGNGAAVRS
                     GALALYETADLDTVEKYCRTTHTHPDAVRTSLAMFIAVRNALNGNQDLTECWK"
BASE COUNT          347 a          210 c          237 g          251 t
ORIGIN      
        1 gagatggtca acggctatct gaaccacaca gaaacactga agattctttt cattctgctc
       61 gacagtgcaa gggaaattcc tgaagaagaa aaggatctga ttcaggtatg tctggaaaaa
      121 aacatacagc ctgttctcat ccgcacaaaa atcgacaaga gaaaccagaa ggaaactgcc
      181 gtactgaaaa aggaaaaaca gatccttgca ctggaatttc ctgaattaaa agtcattttt
      241 gcctcgtcta aaaatggaag aggcctggaa gaaatcagaa acattctttc agaatctctg
      301 tatggataaa aatgaagtgc tcctcagtct gagaaaaatt cccggaatcg gaaaatccat
      361 ttccgaagat ctgtggaaca ttgggattcg gggaatagag gatcttaaaa ataaaaatcc
      421 ttatgatctt tatgaaaagt caaatgaata cgcagggaca gtgcaggaca gatgtcttct
      481 ctatgtattc agaacggcag tctattttgc agaaacacct gaaaacagca gagaacctga
      541 aaagctgaaa tggtggaact ggaaagactg aaaaaacggt gcttcaggct aagatatact
      601 ctgcattcgg attcctgctc ggggatatgt tcggttctcc atttgaatcc agaagctcag
      661 aagaggctgc gaaaggtttc ggacataaag taggttcgta tacagacgat acactgatgt
      721 atctttccgt aatgaaagcc ttcagatatg atctccctct ttccgaaact ctaatcgaat
      781 tttacgacgg aaaacgcgga tacggaagcc ggatggacga aatgctttcc aagaaaatat
      841 gtgcacctac tgattcctgg ggcaacggag ctgcagtaag gtcaggtgca cttgcactgt
      901 atgaaacggc cgatctggat acagtcgaaa aatactgccg caccactcat actcatcctg
      961 acgcagtaag aacctctctt gcgatgttca ttgctgtccg gaatgcattg aacgggaatc
     1021 aggatctgac tgaatgctgg aaaat
//
LOCUS       DAOQGJ010001930         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126163_length_1045_cov_1.584848, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001930 DAOQGJ010000000
VERSION     DAOQGJ010001930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126163_length_1045_cov_1.584848"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..748
                     /locus_tag="PK683_22655"
     CDS             173..748
                     /locus_tag="PK683_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11318.1"
                     /translation="MKKVFLFSIFVFVSLSAEKGRPVRIEGTYKDEFNAFELIISGNG
                     KCQYNQYICGEKLPSRCSYEKKSDTQFLFKTVSVTVFSFTENQEGKDVITEKWSGAPY
                     YKVSDGKTAGIYELSPDYAIDLKKDGKCERISKKDSGETETYKFTCTWKENKGKIVME
                     ELKKSEMNRTETGLNPVSETEIMPAFIKQKN"
     gene            complement(813..941)
                     /locus_tag="PK683_22660"
     CDS             complement(813..941)
                     /locus_tag="PK683_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11319.1"
                     /translation="MVGPGFPGTAAAGIKCFFDRISVNGKSRKDYSPIKGDRISRD"
BASE COUNT          334 a          217 c          238 g          256 t
ORIGIN      
        1 gaaaccagtg cgtctaccaa ttccgccact tcggcttaat tgtcagtgtt tttcaacgtc
       61 ttccagtgtc cataaaaaaa gagacctcaa caaaatacag aaggggcaat tttaagcttc
      121 tcagacgaaa tgttcagact gcaggaagtc taattcaaag agaggtattc gaatgaaaaa
      181 agtattcctt ttctccattt tcgtctttgt ttctttatct gcggaaaaag ggcgcccagt
      241 cagaattgaa ggcacttaca aggatgaatt caatgcgttt gagctgatca tctcaggaaa
      301 cggcaaatgc cagtacaatc agtacatatg cggggaaaaa cttccaagca gatgttccta
      361 tgaaaaaaaa tcagatacac agtttctttt taaaactgtc tcagtcacag tcttttcttt
      421 cactgaaaat caggagggaa aggatgtcat tactgaaaaa tggagcggtg cgccttatta
      481 taaagtttct gacggaaaaa ccgcaggaat ctatgaactc agcccggact atgcaattga
      541 tctgaaaaag gacggaaaat gcgaaaggat ttccaagaaa gattccggag agactgagac
      601 ctataaattt acctgtacat ggaaggagaa caaaggaaaa atcgttatgg aagaactgaa
      661 gaaatcggaa atgaacagaa ctgagacagg cctgaatccg gtcagtgaaa cagaaattat
      721 gcccgccttt ataaagcaga aaaactgatc aaaatgaaaa acagttttct ttaatgataa
      781 tcgtaaagac aggatttccc gcaggcaatt catcagtcac ggctgatcct gtctcctttg
      841 atcggtgaat agtcttttct gctttttccg ttcacggaaa tcctgtcaaa aaaacatttg
      901 attcctgcag ctgcagtgcc cggaaaaccg ggtccgacca agcaagttcg ggaccagcct
      961 gcacgaccga gccgggttcg gcgctaaccg ttgcatcgac aggggttcgt ggaatcgagc
     1021 tggtccgggc gggcgcctgg cggtg
//
LOCUS       DAOQGJ010001931         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126297_length_1044_cov_2.511628, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001931 DAOQGJ010000000
VERSION     DAOQGJ010001931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126297_length_1044_cov_2.511628"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..>1044
                     /locus_tag="PK683_22665"
     CDS             270..>1044
                     /locus_tag="PK683_22665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11320.1"
                     /translation="MKSQPQSVSPLQNKRIFFGTYLPDLAAAFLSGLFSILASRSIYS
                     DILKIENEDLSFQVFKFVLGLIFVSFFILGKQIKKEWVKFEGPVMAVLLTLESAVLLA
                     VSSKEIQVDYIFQPLLFQFFSGLNAAAAGYYISGLKKLRLWLYLAGIVSSYILFYSTF
                     PAPSFTLLMGALVSQILGFILESLSSMKFHIRPLFFQNTRNNRTVTDVFFYSALLLFI
                     SHLGLYYYRGENQPDSLISGGGSALVIFNLIYHFSIAQKY"
BASE COUNT          282 a          235 c          204 g          323 t
ORIGIN      
        1 tacgataagt tcaggaagat ttgcatctcc gggaggaaga taaaaatata caagctggaa
       61 tccaatgata ttgattagaa tggaccagcc taactgaccg attgcatagg cgatctgctt
      121 tgataatggc agcgacggtt tggacatata tgatttctcc cggaataaca gcttgaataa
      181 aagatttcct aaaagaaaat ggcaattcag caaggttttt tatgcaagta ttaattttac
      241 agactccaaa tttttagcga taagacagaa tgaaatcaca gcctcagtcg gtttctccgt
      301 tgcagaacaa gagaatattt ttcggaacat atctcccgga tctcgctgcg gcatttcttt
      361 ccggactctt ctccatactt gcatccagaa gcatttattc ggacattctg aaaattgaaa
      421 atgaagacct ctcatttcag gtattcaaat ttgtgctggg actgatcttt gtaagttttt
      481 ttatactcgg caaacagata aaaaaggaat gggtgaaatt cgagggacct gtcatggcag
      541 ttctgctgac tctcgaatca gctgtgcttc tggctgtctc ttccaaggaa atacaggtgg
      601 actatatctt ccagcccctt cttttccagt tcttttcggg actgaatgct gccgctgcag
      661 gatactatat ctccggactg aaaaaactca gactctggct ttatctggca ggaattgttt
      721 cctcatatat ccttttttac agcactttcc ctgctccgag tttcactctc ctgatgggtg
      781 cactcgtctc tcagatactc ggattcatac tggaaagcct ttcctcgatg aagtttcaca
      841 tcaggccgct tttttttcag aacacaagaa acaacaggac agtgacagat gtttttttct
      901 attccgcact gcttctgttc atttcccacc tggggcttta ctattacaga ggagaaaatc
      961 agcctgattc cctgatctcc ggaggcggtt ctgcccttgt catcttcaat ctgatctacc
     1021 atttctccat agctcaaaaa tatc
//
LOCUS       DAOQGJ010001932         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126309_length_1044_cov_2.426694, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001932 DAOQGJ010000000
VERSION     DAOQGJ010001932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126309_length_1044_cov_2.426694"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PK683_22670"
     CDS             <1..329
                     /locus_tag="PK683_22670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11321.1"
                     /translation="NRQNAFRNQPILPAQPLGQDTFIYNFLVFNDKKYKLEQATARYN
                     ESAAVLFGFWFFNVAQSWLVPWLNSGSAPFSAQLDMRPVYGSQELLAGSARWEKYASF
                     GWKNQF"
     gene            441..>1044
                     /locus_tag="PK683_22675"
     CDS             441..>1044
                     /locus_tag="PK683_22675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11322.1"
                     /translation="MKRTLLKIKSGFLIGVGIALGFATVTILAVAVGNINTFTSGTPV
                     NAAKVNENFASLKTVLENINDPPIGSIVAWHENLSGVPSLPSGWVRCNGQTLSDSLSP
                     LNGQVIPNLNTPKNAWNSKGSFLRGSATSGVFEDDSFQGHWHNAYWATGATGPGYDIP
                     VLGTGGSGGVHQNRFAQTPIDDGANGSPRTASETRSVNMSV"
BASE COUNT          287 a          231 c          247 g          279 t
ORIGIN      
        1 gcaaccggca gaatgctttt cgaaaccagc cgattcttcc tgcacagcca ctgggacagg
       61 atacatttat atacaatttc ctagttttta atgataaaaa atataaactg gaacaggcaa
      121 ctgccagata caatgaatct gctgcagttc tattcggctt ctggtttttt aatgtggcac
      181 agtcctggct agtgccctgg ctgaacagcg ggtctgcccc gttttccgct caactggata
      241 tgcgccctgt ttacggcagt caggaacttc ttgcaggctc cgcgagatgg gagaaatacg
      301 caagtttcgg atggaaaaat cagttttaac gattccttag cgagcgtttt aaaccgctcg
      361 ctaaagtgaa accgctcgct tagaaagaca acactcctaa gagttatgct aaaattttca
      421 aaattgcttt cggaggatat atgaaaagga ctttattaaa aatcaaatcc ggttttttga
      481 tcggggttgg gattgcactc ggctttgcca ctgtaactat ccttgcagta gcagtgggaa
      541 atatcaacac atttacctca ggcacaccgg tgaacgcagc aaaggtcaat gagaatttcg
      601 cgagcctcaa aacagtgttg gaaaacatca acgatcctcc gattggcagc attgttgcct
      661 ggcacgaaaa tctttctggt gttccaagtc tcccttctgg ctgggtacgg tgcaatggac
      721 agactttgag cgacagtctc tctcctttaa acggccaggt cattcccaat ctcaatacac
      781 cgaagaatgc ctggaacagc aaaggaagtt ttctcagagg aagcgcaact tcaggcgtct
      841 ttgaagatga cagttttcag ggacattggc ataatgctta ttgggcaaca ggtgcaacag
      901 gtccagggta tgatattcct gttttaggga ctggtggaag tggtggtgtt catcaaaatc
      961 gatttgcaca aactccaatt gatgatggtg caaacggttc ccccagaaca gcttcggaaa
     1021 cccgttctgt gaatatgagt gtgg
//
LOCUS       DAOQGJ010001933         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126317_length_1044_cov_2.385238, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001933 DAOQGJ010000000
VERSION     DAOQGJ010001933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126317_length_1044_cov_2.385238"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PK683_22680"
     CDS             complement(<1..616)
                     /locus_tag="PK683_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11323.1"
                     /translation="MDSFRPKPMFNRSELRQIKRSRTKISGRRVITDDVKKLKSLKVT
                     PDLPEEEAVNYYREPIWIEYYIPKESRFALETKYLFIKLIDPVPREGKDEVLRKAQTE
                     DEFIDLTVYFDERTPEAETVKKSYYNYTSMIQTISDHVKMYRQTENPEYLITPVYLIE
                     TMMMYEPTLAALEYLGDYHLHNLHWLIRRLNRGQVEFSLEDRTIL"
     gene            677..>1044
                     /locus_tag="PK683_22685"
     CDS             677..>1044
                     /locus_tag="PK683_22685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11324.1"
                     /translation="MHIFLFILIPFLSLYSQEKEFPAEKQDSPTLKRPVNDKNPAAAA
                     LLGFVPGLGQAYLGNIPSAGIQAGMFLAISGIRSDFASRPDYIDYENREVKFDYKDAL
                     FGYYAQRNGLVYNNDLTGTVL"
BASE COUNT          262 a          242 c          227 g          313 t
ORIGIN      
        1 gaaggattgt tctgtcttcc agtgaaaact ccacctgacc ccggttgagt ctcctaatca
       61 gccagtgcag attgtgaaga tgatagtccc ccagatactc aagagctgcc aaagtcggct
      121 catacatcat cattgtttca atcaggtaca ccggcgtgat cagatattcc ggattttcag
      181 tctgtctgta cattttcaca tgatctgaaa ttgtctgaat catggaagta tagttgtaat
      241 aggacttttt tacggtttcc gcctccggcg tcctttcatc aaaatacaca gtcagatcaa
      301 tgaattcgtc ctccgtctga gccttcctca gaacctcatc ttttccttct ctgggaaccg
      361 ggtctatgag ttttatgaaa aggtacttgg tttcaagggc gaacctggat tcctttggaa
      421 tatagtattc aatccagatg ggttctctgt aataattgac tgcttcctcc tcaggaagat
      481 ccggagtgac cttgagggat ttgagctttt ttacatcgtc agtgatgacc cgtcttccgg
      541 agatctttgt ccggcttctt ttgatctgcc gaagttcgct ccggttgaac atcggttttg
      601 gtctgaagga atccactcta tttttatcgg gcagaaaaag tttacagata acaaaaatgc
      661 aaaaactctg tattctgtgc acatatttct attcattcta atccctttcc tttcccttta
      721 ctcacaggaa aaggagtttc cagccgaaaa acaggacagc ccgacgttga aaagacctgt
      781 gaatgacaaa aatcctgccg ccgctgccct gctcggattt gttccgggct tgggacaggc
      841 atatctggga aacattcctt cagccgggat tcaggcaggg atgttccttg cgatttcggg
      901 aatccgaagc gatttcgcat ctcggcccga ctatattgat tatgagaaca gggaagtgaa
      961 atttgactac aaggatgctc ttttcggata ttatgctcag agaaacggac ttgtatacaa
     1021 taacgatctg accggcactg tcct
//
LOCUS       DAOQGJ010001934         1043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126485_length_1043_cov_3.177126, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001934 DAOQGJ010000000
VERSION     DAOQGJ010001934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126485_length_1043_cov_3.177126"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PK683_22690"
     CDS             complement(<1..421)
                     /locus_tag="PK683_22690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HNH11325.1"
                     /translation="MKYSIIKIQEEIRNGSPLEFLFFWENRPVSGKEAFEETLSQWWE
                     SRFEKDGIIYSSAEKWMMAEKARLFGDDDVLRQILLSDNPEKIKKLGREIRGFDHEIW
                     DKNKYSIVMEGNVLKFSQDEKLKNILLKTGDKILVEAS"
     gene            complement(418..>1043)
                     /locus_tag="PK683_22695"
     CDS             complement(418..>1043)
                     /locus_tag="PK683_22695"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNH11326.1"
                     /translation="RLERDLDFVLVDSELGIGNGFCIPAGFLREPESALSRAHSVVFT
                     KYEKGHTSEADRLEERWKVQFRHLRFFHLSYLPDRICSLKGNTVPENILTGRKLVLFT
                     GIARSESFFRTAGKIQDAVIYDTVSFRDHYSFRKDDIDALSKKFGNDSVFLCTEKDAV
                     KLSYFKDAISGLAVYYLSLKTEITEKNEFEEFVMNRISESSVKSRMQ"
BASE COUNT          281 a          249 c          182 g          331 t
ORIGIN      
        1 gacttgcctc cacaagaatc ttatctccag ttttcagaag gatgtttttc agcttttcat
       61 cctgtgaaaa tttcagaaca ttgccttcca ttacgatact atatttattt ttatcccaaa
      121 tttcatgatc aaatcccctg atttctctgc ccagtttctt tattttttcc gggttgtcag
      181 acaggagaat ctgtctcagt acatcatcat ctccgaaaag ccgggctttc tccgccatca
      241 tccatttttc agcagagctg tagatgatcc cgtccttttc gaatctggac tcccaccatt
      301 gactcagagt ttcttcgaaa gcttcttttc ccgaaacagg tctgttctcc cagaaaaaca
      361 gaaactccag cggactgcca ttccttattt cttcctgaat tttaattatg ctgtatttca
      421 ttgcatcctg cttttaacgg aactttcgga aatccggttc attacaaact cttcgaattc
      481 attcttctct gtaatttcag ttttcagaga aagataatat acagcaagtc cggaaattgc
      541 atccttgaaa taagaaagtt tgacagcatc cttctctgtg cagagaaaaa cggaatcatt
      601 tccgaatttt ttggagagag catcaatatc atcttttctg aatgaataat gatccctaaa
      661 tgatactgta tcataaatta cggcatcctg aattttcccg gctgttctga aaaaagattc
      721 cgatctggca atccctgtaa aaagaacaag tttccttccg gtcagaatat tctccgggac
      781 agtattccct ttcagactgc agatcctgtc cggcagatag gagagatgaa agaatcggag
      841 atgtctgaac tgaactttcc atctttcctc aagtctgtct gcctctgaag tatggccttt
      901 ttcgtattta gtgaacacga ctgaatgagc ccgtgaaagt gcactctccg gctcgcgcag
      961 aaagcctgca ggaatgcaga atccgtttcc gattcccagt tcagaatcaa caaggacaaa
     1021 atccaggtct ctttccagtc tgt
//
LOCUS       DAOQGJ010001935         1043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126499_length_1043_cov_2.921053, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001935 DAOQGJ010000000
VERSION     DAOQGJ010001935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126499_length_1043_cov_2.921053"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK683_22700"
     CDS             <1..415
                     /locus_tag="PK683_22700"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390366.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HNH11327.1"
                     /translation="GRVDDVINVSGHRLGTAEVESALVEHPGVAEAGVVGYPHDIKGQ
                     GIYAYVTVKNGVSTNDELKKELIAIVEKIIGKIARPDVIHWAPGLPKTRSGKIMRRIL
                     RKIAAGEFDGLGDISTLADPSVVKNLIEDKKKYHS"
     gene            420..998
                     /locus_tag="PK683_22705"
     CDS             420..998
                     /locus_tag="PK683_22705"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH11328.1"
                     /translation="MIELQNPDPHSRAEILSAIDDAVDQASSFYTSLGPEIFFHDGLG
                     GWSPAQNISHIADITSLAVLLFRMPKFFLLPFGRRKTQKNFSTLLSEYIGAEKPIFIG
                     PLAPSSIPLPENAQETVQKLTARFRNMFQDLKKVLQEIPEEEFDRYSMPHPSLGMLSF
                     REMVYVVIIHIIHHTYKVEKKTGRLIKKAAPI"
BASE COUNT          305 a          228 c          234 g          276 t
ORIGIN      
        1 aggccgggtg gatgatgtca tcaatgtatc cggtcacagg ctcggaactg cagaggtcga
       61 aagtgctctt gtggaacatc cgggcgtggc agaggcaggg gttgtaggat atcctcacga
      121 catcaaggga caaggaattt acgcatatgt cactgtaaag aacggagttt ccacgaatga
      181 tgaactcaag aaagaactca ttgcgattgt ggaaaagatc atcggaaaga ttgcaagacc
      241 ggatgtaatt cactgggctc ctggtcttcc gaaaacgcgg tcggggaaaa ttatgagaag
      301 aattcttagg aaaattgcgg ccggagagtt tgacggtcta ggcgacatat cgactttggc
      361 agatccttcc gttgtcaaga atctgattga agacaagaaa aaataccata gctgataata
      421 tgatagaact acagaatccc gaccctcatt ccagagcaga aatcctttct gcaatagacg
      481 atgctgtcga ccaggcatct tccttctaca cttctctcgg tcctgagata ttttttcatg
      541 atggactggg aggctggtct cctgcgcaga atatttctca tattgcagac attacctccc
      601 tggcagttct gcttttcagg atgccaaaat ttttccttct cccgttcggc aggagaaaaa
      661 cacagaaaaa cttcagcact cttctttcgg aatacatcgg tgcggaaaaa ccgattttca
      721 ttggacctct tgcaccatct tccattcctc ttccggaaaa tgcacaggaa acagttcaga
      781 aactgactgc cagattcaga aatatgtttc aggatctgaa aaaagtactg caggaaattc
      841 ctgaggagga atttgacaga tactcaatgc ctcatccctc cctcgggatg ctcagcttcc
      901 gggagatggt ctatgtagta atcatacata ttattcatca tacatataaa gtagagaaaa
      961 aaacaggcag acttataaaa aaggcggctc cgatatgaaa ttcaggttct tatcgtacct
     1021 tctttttctt ctccttgttt cat
//
LOCUS       DAOQGJ010001936         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126693_length_1042_cov_3.478217, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001936 DAOQGJ010000000
VERSION     DAOQGJ010001936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126693_length_1042_cov_3.478217"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..445
                     /locus_tag="PK683_22710"
     CDS             26..445
                     /locus_tag="PK683_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HNH11329.1"
                     /translation="MWRKSHSAKIEGVSAEKVWKVWADVNQWNVWQDDIDYAKMEGRF
                     QAGNKFLLKPKGGPKVNIEILEAVENRGFIDLTRFPFARMYGSHQFTEKNGVLEMETS
                     ISIEGPLAFLWKKIVAEDIVKKLPEQTAALIKRAQNV"
     gene            438..902
                     /locus_tag="PK683_22715"
     CDS             438..902
                     /locus_tag="PK683_22715"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNH11330.1"
                     /translation="MSEKTVFKEKDADDSPGFLLFKITVLWQKKLGIIFNDLRIQQTQ
                     YAILASLKFYEEKNEKCTQAFLVEHARIDKMTLSKSVRQLEKSGLVRRTQSEEDARAV
                     IVNLTSKGRKLVTEAVQRIEDADESFFQYLTEKQRCSFKEISLALIKGNSES"
BASE COUNT          353 a          174 c          259 g          256 t
ORIGIN      
        1 cccagtttac taacaaggag agcttatgtg gagaaagagt cattctgcaa aaatcgaagg
       61 ggtttctgca gaaaaagtat ggaaggtctg ggcagatgtg aatcagtgga atgtctggca
      121 ggatgatatt gattatgcaa aaatggaagg cagatttcag gctggaaata aattccttct
      181 gaaaccgaag ggcggaccga aggtgaatat tgaaatactg gaagctgtcg agaatcgggg
      241 atttattgat cttaccagat ttccgtttgc cagaatgtac ggatctcatc agtttacgga
      301 aaagaacgga gttttggaga tggaaacttc catcagcatc gaaggacctc ttgcattcct
      361 ctggaaaaaa attgtagcag aggatattgt aaaaaaattg cctgagcaga ctgcggcatt
      421 gatcaaaaga gcgcagaatg tctgaaaaaa cagttttcaa ggagaaggat gccgatgaca
      481 gtcccgggtt ccttctcttt aagattactg tcttgtggca gaaaaagcta gggataattt
      541 ttaatgattt aaggatacag cagacacagt atgcaatcct tgcctcattg aaattctacg
      601 aggaaaaaaa tgaaaaatgc actcaggctt ttctggtaga acatgcaaga attgacaaga
      661 tgacactttc aaaatcagtg cggcagctgg aaaaatcggg actggtcaga agaactcagt
      721 cggaagaaga tgcaagggct gtaattgtga atcttacatc taaaggaaga aagcttgtga
      781 ctgaggcagt gcagagaata gaggatgcag atgaatcatt tttccaatac ctgactgaaa
      841 aacagaggtg ttccttcaag gaaatctcac tggcactgat caagggaaat tcggaatcct
      901 gaaaattttg aatctgtaaa ctgaaaaaca aatttcggat gaacagctcc aactgtatgc
      961 agacattttc cgacattgca gattcagatt accgtgaggc aaaaatgagc cgcaaaggaa
     1021 ctggcctgat gcatggatgt gt
//
LOCUS       DAOQGJ010001937         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126735_length_1042_cov_2.788247, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001937 DAOQGJ010000000
VERSION     DAOQGJ010001937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126735_length_1042_cov_2.788247"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PK683_22720"
     CDS             complement(<1..508)
                     /locus_tag="PK683_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichol kinase"
                     /protein_id="HNH11331.1"
                     /translation="MDGFNIHRKLFHLIGFAVPASLYFDICSGAFGLKYATRAILFTV
                     LAAVLISMSFSEFIRLHNSAFNEFFQKYFGKLMKEAEKNRMNGTIPYILSNALVVAFF
                     PAQIIFLAMGCLLIGDPVAAFFGSKYGKYRFYNGKSLVGVVAFIIGSIISCTILMFLF
                     QATYGDSLY"
     gene            complement(517..870)
                     /locus_tag="PK683_22725"
     CDS             complement(517..870)
                     /locus_tag="PK683_22725"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH11332.1"
                     /translation="MELNNLDVFVERIENIAVMRVEGVINSLTSQKFLEEVKHAGRKG
                     GLIIDMEEISLISSMGIEVLKKVSDWSYTSGNRIVLLNLSQPVKQTLNMLGMLRIFSI
                     APNEEIAMKMVTKVK"
BASE COUNT          326 a          234 c          200 g          282 t
ORIGIN      
        1 aatacagact gtctccgtaa gtcgcctgaa aaaggaacat caggatcgtg cacgaaatga
       61 tagaaccaat gatgaatgcc actaccccta ccagtgactt tccgttgtag aagcggtatt
      121 tgccgtattt ggagccgaaa aaggcggcaa ctggatcgcc tatcagcaga cagcccattg
      181 ccagaaaaat gatctgagcc ggaaaaaaag caaccacaag tgcattggac agaatgtaag
      241 gaattgttcc gttcatcctg ttcttttccg cctctttcat cagcttgcca aagtattttt
      301 ggaaaaactc attgaatgca gaattgtgaa ggcggataaa ttctgaaaag gacatggaaa
      361 tcagaactgc ggccagaaca gtaaacagga ttgcccttgt cgcatactta agtccaaaag
      421 ccccagaaca gatatcaaaa tacaatgatg cgggaacagc aaatccgatc agatgaaaca
      481 attttcggtg aatgttaaaa ccatccatca aatccctcat ttaaccttag tgaccatttt
      541 catggctatt tcttcatttg gagctatact gaaaatcctg agcattccga gcatattcaa
      601 tgtctgcttt actggctgag aaaggtttaa gagaacaatt ctgtttccgg aagtataact
      661 ccagtcactg acttttttca gtacctcgat gcccatcgaa gaaatcaggc tgatttcctc
      721 catatcgatg atgagcccgc cctttctgcc ggcatgtttg acttcttcga gaaacttctg
      781 agatgtaaga gaattgatta caccctctac ccgcattact gcgatatttt cgatcctttc
      841 cacaaaaacg tcaagattgt tcagctccat ttgtataaaa ctatcctgta cactgcagta
      901 ccttgcaaag tgatttttta atgcccaaac ccaatttttt cttgaaaaaa ataagcctgg
      961 gaattacact tatgccacag gctgacagga ggatatatga acgtcaagga tctctattcc
     1021 atctatgaat cgatcaatga ag
//
LOCUS       DAOQGJ010001938         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126771_length_1042_cov_2.439716, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001938 DAOQGJ010000000
VERSION     DAOQGJ010001938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126771_length_1042_cov_2.439716"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PK683_22730"
     CDS             complement(<1..644)
                     /locus_tag="PK683_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11333.1"
                     /translation="MNVLYNRLFVFGLIVISAVICSMNSWFIFFPGKAALYFVFLSIP
                     FLFLPEFRYRAVFSASAIFAGSVIFFILFYPVRNISLGDGELLYENFLLENSVWGYQL
                     VLDEIFEAVLHSSVFQILKLSDPSEAYRILSTGAGLVLVLFLFFRADQKDRYSLLPIL
                     TSGGMLLFFGYMENYTLTALIIFLYAFTALHFLDRNLMNERNFLFLCLISALSFV"
     gene            complement(641..>1042)
                     /locus_tag="PK683_22735"
     CDS             complement(641..>1042)
                     /locus_tag="PK683_22735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11334.1"
                     /translation="MKSVKKASYESYRKRYVSGLKTGAKFKEDFTLFSSVYRYSSQDS
                     FFLMFTDDTVLLRPLYGILNLSAGTLYSAGAVFYAPFDRAKRLKKGGESLLFSLPELI
                     FFNIRKGTFISAEDYDRIFFGEDFGTEETDS"
BASE COUNT          347 a          217 c          220 g          258 t
ORIGIN      
        1 acgaatgaca gtgcagaaat caggcataag aaaagaaaat tcctctcgtt catcagattt
       61 ctgtccagaa aatgaagggc tgtgaatgca tacagaaaaa tgataagcgc tgtaagagta
      121 tagttttcca tgtatccgaa gaaaagcagc attcctcccg atgtcagaat gggaagcaga
      181 gagtatctgt ctttttggtc tgcacggaaa aacagaaaga gcacaaggac aagccctgct
      241 cctgtgctca gaatcctgta tgcttcagag ggatcagaga gtttcagtat ctgaaatacg
      301 gaggaatgca gaacagcctc gaatatttcg tccagaacaa gctggtatcc ccagacagag
      361 ttttcgagaa gaaagttttc atacagaagt tctccgtctc ccagactgat gtttcttaca
      421 ggataaaaaa ggataaaaaa tatcacagaa ccggcaaaaa tggcggatgc agaaaacact
      481 gccctgtatc tgaattcggg aaggaaaaga aaggggatgc tcaggaacac gaaataaagc
      541 gcagcctttc ctggaaaaaa aatgaaccag ctgttcatgc tgcagatcac tgcactgatg
      601 acaataagtc caaatacaaa tagtctgtta tacagtacgt tcaactgtct gtctcctctg
      661 ttccaaaatc ctctccgaag aatatccggt cataatcctc tgcagaaata aaagtgcctt
      721 tccttatatt gaaaaaaatc agttcaggca gactgaagag aagactttct ccgccttttt
      781 tcagtctttt agccctgtca aaaggtgcat aaaaaactgc ccctgcagag tacagggtgc
      841 ctgcgctgag attcagaatg ccgtacaagg gtctcagtaa aacagtatcg tcagtgaaca
      901 tcagaaaaaa agaatcctga ctgctgtatc tgtacacgga tgaaaacaga gtaaaatctt
      961 ccttgaattt tgctccggtc ttcagacctg aaacatatcg ttttctgtag gattcatagg
     1021 atgccttttt cacagatttc at
//
LOCUS       DAOQGJ010001939         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126795_length_1042_cov_2.257345, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001939 DAOQGJ010000000
VERSION     DAOQGJ010001939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126795_length_1042_cov_2.257345"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PK683_22740"
     CDS             complement(<1..766)
                     /locus_tag="PK683_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNH11335.1"
                     /translation="MNEHRLSLLEETKGSVSIRNFSRLLVTASVFIFLSLCILPWQQT
                     VSGYGRVVAYAPLDRQQFIEAPIDGRIVHWHVQEGSNVKKDDLIVEISDNDPMFLQRL
                     QEEKSAIESRIAAVKARSEAIQARIRSLEGSVGNSVSAAKSRTRMARDRIDASEHALE
                     AAAAVYRTASINSKRQKTLFAQGLASQRAVETSEMEEARALTDLNRAKAAVSSSKSEL
                     LALQSDQSKAGTDGSASIEDAKASYAAALAELASAQA"
     gene            complement(759..>1042)
                     /locus_tag="PK683_22745"
     CDS             complement(759..>1042)
                     /locus_tag="PK683_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11336.1"
                     /translation="ICSEFGEDFHSLDISEFTERLNEDDLFLFYTALVWRNSVSLAIL
                     CPPGYIGENTLSALNSFIQNHGMEVIVIHCNDKIISNKSFEIMERKKDE"
BASE COUNT          274 a          259 c          223 g          286 t
ORIGIN      
        1 ctgcctgagc acttgccagt tctgcaagtg cagcagcata ggatgctttt gcatcttcaa
       61 tggaagcaga cccgtcagtg cctgccttgg actgatcaga ctgaagtgca agcagttctg
      121 acttggaaga actgactgca gccttggcac ggttcagatc tgtaagagcc cgggcttcct
      181 ccatttcaga agtttccact gctctttggg aagcaagccc ctgcgcaaaa agagtcttct
      241 gtcttttaga attgatgctc gcagtcctgt atactgccgc tgccgcctcc aatgcatgtt
      301 cagatgcatc aatcctgtcc ctggccattc ttgttctcga ttttgcggca gaaacagaat
      361 tgccgacaga accttcaagc gaacggattc tggcctgaat cgcttcgctt ctggccttga
      421 cagctgcaat cctggactct attgcagact tttcctcctg cagcctttga agaaacatag
      481 gatcattatc cgatatctcc acaatgagat catccttctt tacgttgctt ccttcctgta
      541 catgccagtg gacaatcctt ccgtcaatcg gagcttcaat gaactgctgt ctgtccagcg
      601 gagcataagc aacaacccgc ccataccctg acacggtctg ctgccaggga agtatgcaca
      661 gcgaaagaaa gataaagaca gatgctgtta caagaagtct ggaaaaattt cggatggaaa
      721 ctgaaccctt ggtttcttcc agaagagaca gtcggtgctc attcatcttt ttttctctcc
      781 attatttcaa aagatttatt tgatattatt ttatcattac agtgaattac aatgacttcc
      841 atgccatgat tctgaataaa gctgttcagt gcggaaaggg tgttctctcc tatgtacccc
      901 ggcggacaga gtattgcaag agagacagaa tttctccaga caagcgctgt ataaaaaagg
      961 aaaagatcat cctcattcag cctttcggta aattcagaaa tatcgagtga atgaaaatcc
     1021 tcgccgaatt cagagcagat tc
//
LOCUS       DAOQGJ010001940         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_126803_length_1042_cov_2.214792, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001940 DAOQGJ010000000
VERSION     DAOQGJ010001940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126803_length_1042_cov_2.214792"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PK683_22750"
     CDS             complement(<1..428)
                     /locus_tag="PK683_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11337.1"
                     /translation="MLNCRSTEYRSPEDSLKSYFADRSRDLGDIFYFGVEDKVHGASI
                     FFAYFPIGHGYNMEGKGIGLMGGAFDFYEIGDRTNPISIYMADPDGKEEKDEIYFGQQ
                     TFGYSAAALFRSENPIINKRNKNNAMFVSHTVCYKCKWKAV"
BASE COUNT          286 a          226 c          198 g          332 t
ORIGIN      
        1 actgccttcc atttgcattt atagcatact gtatgtgata caaacatagc attattctta
       61 tttcgtttat tgatgatagg attttcgctt ctgaacaaag ctgcagctga atacccaaaa
      121 gtctgctgtc caaaatagat ttcgtctttc tcttctttcc cgtctggatc cgccatatat
      181 attgaaatcg gattggttct gtctccgatt tcatagaaat caaaagcacc acccatcaga
      241 ccaattcctt tcccttccat attgtatcca tggccgatag gaaaatatgc gaagaatata
      301 cttgccccat gaaccttgtc ctcaactccg aaatagaaaa tgtcacccag atctcttgaa
      361 cggtctgcaa aataggactt cagcgaatct tcaggtgatc ggtattccgt gcttctgcag
      421 ttcagcatca agagtatcag cagaaagaaa aacattcttc tcatattcat ttgacttatg
      481 gcagaatgag tagttctatt ttttattcct cacctgacaa ggttgttttt tggatctgtc
      541 atcggtgcga tgaagtaaaa atgcggcttc tggaattttc tgttttgatg caaaggactt
      601 gttcgggttc aggcccgcac atccttcgtg tacagaattt ctttcgcaga gtcttctgga
      661 tgtatgcaga cagtcattga cctgaaaaaa ctgtacgaag aggatgaaca tctgagcgca
      721 tttcccgaag atcccccatt tacttttgaa caggtaacgg atgaaaaata tcttcctgaa
      781 taaatcagtg ctctgcgaac tcttcgtctt tgcgtgaaac ttattctctt tcctttaggc
      841 aatcctgaat ctgcttgaca gtcttctgaa gcaatgtctt ctttgatcta tgaacgcact
      901 ttctttcaga attccggatt atccgattat ggatgatgac cgtttgtaca aattctgcgt
      961 ttataatcgc gagatgaaaa ttgaacgttc ttcacaggga gaaattacag ttatgtcacc
     1021 gacaggcgga gaaagcagca gc
//
LOCUS       DAOQGJ010001941         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127175_length_1040_cov_3.250761, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001941 DAOQGJ010000000
VERSION     DAOQGJ010001941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127175_length_1040_cov_3.250761"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1040)
                     /locus_tag="PK683_22755"
     CDS             complement(<1..>1040)
                     /locus_tag="PK683_22755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11338.1"
                     /translation="AFRLSESFAGALLAGLIFGWSGSITAQVTMAVIPVSSSWLPLLT
                     LSHLLIFEQPNSRARFLFNAAITYLSVTSGSPQTVFFNFLFAYLFLAFKIRYPRIPFL
                     NRKNGGAISDRTGNEKLELKPVYTVLLSTGAGILLSSVSLFPLIELMKYSDRSVSIDK
                     AAFNSYHFSFSDLIKIPFPYSFGGLTENSLFRFSYIYPSTAIDNFHEHQRYMGIISFI
                     IAFIGWKNTEDRRLKAFLASGFIFYLLYSLGTETPLGRLIYGIPVISKFRGPNRHILE
                     LTLIVSILAAFGIRRIAENGRYAGPKERFHFLYFTFFFILALFTAVLSVPDLKSRYSA
                     VNFFSFYGNNLLT"
BASE COUNT          338 a          187 c          246 g          269 t
ORIGIN      
        1 gtgagcagat tgtttccgta gaaactgaaa aaattgaccg cagaatatct gcttttcaga
       61 tcaggtaccg acagcactgc agtgaaaagt gccagaatga aaaaaaatgt gaaatagaga
      121 aagtgaaatc tttcttttgg ccctgcgtat cttccgtttt ctgcaatcct tctgattccg
      181 aatgcagcaa ggatggaaac aatcagagtc agttccagta tatgtctgtt gggtcctctg
      241 aatttgctga ttacaggaat accgtaaatc agtctgccta aaggagtttc tgttccaaga
      301 gaatacagaa ggtagaaaat aaaccctgaa gctagaaagg ctttcaggcg tctgtcttca
      361 gtgtttttcc atccgataaa tgcaatgata aaggagatga ttcccatata acgctgatgt
      421 tcatgaaaat tatcaatcgc agtagaggga taaatgtagg aaaaacgaaa gagactgttt
      481 tctgtcagac ctccgaaaga ataagggaag ggaattttta tcaggtcaga aaatgaaaaa
      541 tgataagaat taaatgcagc tttgtctatg gaaactgatc tgtctgaata tttcatcagt
      601 tcaatcagcg gaaagaggga aactgacgag agcagtattc ctgcgcctgt cgaaaggagg
      661 acagtgtaca caggttttaa ttccagtttt tcatttccag ttctatcaga aattgccccg
      721 ccgttctttc tgttcagaaa aggaattctc gggtatcgga ttttgaaagc tagaaagaga
      781 tatgcaaaga gaaaattgaa aaagactgtc tgcggacttc ccgaggtgac tgaaagatat
      841 gtgatcgcag cattgaaaag gaatctggca cggctgttcg gctgttcaaa gatcagaaga
      901 tgtgaaagtg taagaagagg gagccaggaa gatgagactg gaatgacagc cattgtcacc
      961 tgagcagtga tgcttccgct ccatccgaaa atcagtcctg ccagaagtgc tcctgcaaaa
     1021 ctttcggaaa gcctgaatgc
//
LOCUS       DAOQGJ010001942         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127271_length_1040_cov_2.208122, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001942 DAOQGJ010000000
VERSION     DAOQGJ010001942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127271_length_1040_cov_2.208122"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PK683_22760"
     CDS             <1..651
                     /locus_tag="PK683_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11339.1"
                     /translation="VDGYVFSEISVLNELKHYTESRLTQVYESTFYKKKTGFQILREL
                     YPRAKEYNNTNLGRALNESHMVEEYIKQVSSNAFEFTESWKKEKLYKLYREEEDAAYA
                     DSKTGYDAGSMRNDYKNLKEPNSKWAKAVRQFSVKFLIRIHFRKYEFDVVKKLILSGK
                     IYDIEDMIYIRNSILELEKFLDKDPILNYHLEKMTDLRRICQAKINVSRKSVFGKN"
     gene            720..>1040
                     /locus_tag="PK683_22765"
     CDS             720..>1040
                     /locus_tag="PK683_22765"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH11340.1"
                     /translation="MNYKIEEIRVSGGTANLINFQTNDQNSLTRANMKELGDILDEIK
                     KNDQIKAVVFSSENPKFFCNGLDAETLLNTSREELPEAVGGIVLLFGRILQFDKPIIT
                     EVTGH"
BASE COUNT          376 a          184 c          214 g          266 t
ORIGIN      
        1 gtggacggat atgtgttttc cgaaatcagc gtactgaatg aactgaaaca ctataccgaa
       61 tccagactta ctcaggtata tgaaagcact ttctataaaa agaaaacggg ctttcagatt
      121 ctgcgggagc tttatcccag ggcaaaggaa tacaataata caaatctggg aagagcattg
      181 aacgaatccc atatggttga agagtacatc aagcaggtca gctcgaatgc atttgagttt
      241 actgaatcct ggaagaagga aaaactttac aaactctacc gcgaggaaga agatgcggcg
      301 tatgcggatt caaagaccgg gtatgatgca ggatcaatga gaaatgacta taaaaatctc
      361 aaagaaccga attcaaaatg ggcaaaggct gtcagacagt tctctgtaaa gtttctgatc
      421 agaattcatt tccgcaaata cgaatttgat gtagtaaaaa aactgatact ttcagggaaa
      481 atatatgata tagaagatat gatctatatc agaaactcaa tacttgagct ggaaaaattt
      541 ctggacaagg atcctattct gaattatcat ctggagaaaa tgactgatct tcgaagaatc
      601 tgtcaggcaa aaatcaatgt ttccagaaaa agcgtcttcg gaaaaaactg atttatattt
      661 cctttttaag attcaaaaaa agcagatgac acctgatatt ttttttcgat aatagaaata
      721 tgaactataa gatcgaagaa atcagagtgt ccggcggaac agccaacctt attaattttc
      781 agacaaatga tcagaattca ctgacccgtg caaatatgaa agagctcggc gacatcctcg
      841 atgaaatcaa aaaaaatgat cagataaaag cagtcgtttt cagctcggag aatcctaaat
      901 ttttctgcaa tggacttgat gctgaaaccc ttctgaatac aagcagagaa gaactgcctg
      961 aggcagtggg cgggatagtt cttctcttcg gaagaattct gcagttcgac aaaccgatta
     1021 tcactgaagt aacaggccat
//
LOCUS       DAOQGJ010001943         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127442_length_1039_cov_2.754065, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001943 DAOQGJ010000000
VERSION     DAOQGJ010001943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127442_length_1039_cov_2.754065"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PK683_22770"
     CDS             complement(<1..1018)
                     /locus_tag="PK683_22770"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNH11341.1"
                     /translation="MGELDQCKAIAEEGKKREGSLYTLFSVFSDSVSDKQLQDSMIDF
                     AFRLPLDFPGEYDFIFEKFRPESLVISAWDLWLNMILSAHKNNAKIYLVNATLSSSSG
                     RMNPIAKRLTQITVSKMSGLAPVSEESEIQFRSILGNTNIPILRLGDSRFDSVSAKIE
                     KNLEKNDPLKGYEKNRNVIILASTYSACEEMIFPVLKELLSRGRKIWIFSHKVHPERI
                     QNIAERLNGLSLKFSRFSEEGIKALDSEIAVFDVLGVLAFAYSKAGFAYVGGALHNRV
                     HNVIEPAYFGLPVLAGPKIFNSAEAQELRKRGGLWTVNTAAEFLEKEKYLSDGAHYRL
                     AEKTN"
BASE COUNT          289 a          247 c          209 g          294 t
ORIGIN      
        1 agtttgtctt ttctgcaagc ctgtaatgag ccccatccga aagatatttt tccttctcca
       61 ggaattcagc ggcagtgttt actgtccaga gcccgcccct ttttcgaagt tcctgagcct
      121 ctgcggaatt gaaaatcttc ggcccggcaa gaaccggaag accgaaatag gcaggctcaa
      181 tcacattgtg aacccggttg tggagagcac cccccacata ggcaaaacct gccttggaat
      241 aggcaaatgc aagcacgccc agaacatcaa aaaccgcaat ctcggaatcc aatgccttga
      301 ttccttcttc agagaacctc gaaaacttca gagacaggcc attcagacgt tctgcaatat
      361 tctgtattct ttcaggatga accttgtgcg aaaaaatcca tatcttcctt cctctggaaa
      421 gaagttcttt cagaactgga aaaatcattt cctcgcatgc agaatatgtg gaagcgagaa
      481 taatcacgtt tctgtttttt tcatatcctt tcaatggatc gttcttttcg agattctttt
      541 ctatttttgc agatacagaa tcgaacctgg aatcccccag cctcagaatt ggaatattcg
      601 tatttcctaa tatggatctg aactgaattt cagattcttc ggagaccgga gcaaggccgg
      661 acatttttga aacagtgatc tgagtgagtc tttttgcaat cggattcatc cttcctgaac
      721 tgctgctcag agtcgcattc acaaggtata ttttagcatt atttttatgt gcagaaagta
      781 tcatattcag ccagagatcc caggcagaaa tgacaaggct ttcaggtctg aatttttcga
      841 agataaaatc gtactctcct ggaaaatcga gtggcagacg gaatgcaaag tcaatcatcg
      901 aatcctgaag ctgcttatca cttacactgt cggagaaaac agaaaaaaga gtgtagagac
      961 ttccttctct tttttttcct tcctctgcta tcgccttgca ctgatcgagt tcacccaccg
     1021 aggcggcatg gaaccagac
//
LOCUS       DAOQGJ010001944         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127452_length_1039_cov_2.649390, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001944 DAOQGJ010000000
VERSION     DAOQGJ010001944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127452_length_1039_cov_2.649390"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PK683_22775"
     CDS             <1..781
                     /locus_tag="PK683_22775"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNH11342.1"
                     /translation="NVMRLTNASAGGSSWRLFIGDSGSGLSNKFLITYVGSAGGGILG
                     GDAYNMMTFDKSTGNVGIGTTSPSANFQVVGTTLATAWSTSDERYKKNIQQIDNPVEK
                     IMNLRGVNYEWKREEYKNLKFKEGKDFGFIGQEVEKVLPETVFKDNKGYLYVSYQTIL
                     PVLVEAFKKQTVQIRTTNEYLRSMSDSLETEKSRSQKLETAVRKLSDELASVRMHGRA
                     SEQGASPRSASQQENEALKKELADMKNRLRIIEQMQIAGKQ"
BASE COUNT          342 a          203 c          256 g          238 t
ORIGIN      
        1 aaatgtaatg agacttacaa atgcttcagc aggaggttca agctggaggt tatttattgg
       61 agacagcgga tccggattga gtaataaatt cttgatcacc tatgtcggga gtgccggcgg
      121 aggtatatta ggaggagacg cttacaatat gatgactttt gataaaagta caggcaacgt
      181 cggcatcggc acaacttcac catctgcaaa ttttcaagta gtcggcacta cactggccac
      241 agcctggagc acgtcggatg aacgttacaa aaagaacatt cagcagatag acaatcctgt
      301 ggaaaaaatc atgaacctgc ggggcgtgaa ctatgaatgg aagagagagg aatacaagaa
      361 cctgaaattt aaggaaggaa aggacttcgg attcatcggt caggaagtgg aaaaggttct
      421 gccggaaact gtcttcaaag ataataaagg atacttatat gtttcttacc agaccattct
      481 tcctgttctg gtagaggctt tcaagaagca gactgttcag atccgcacaa cgaacgagta
      541 tttgcgttca atgtccgact cattggaaac agaaaaatcc agaagtcaga aactggaaac
      601 tgctgtccgc aaactgtcag acgaactggc ctcagtaagg atgcacggcc gtgcgtccga
      661 acagggtgcg tctccacgaa gtgcatccca gcaggaaaat gaagcactga agaaggaatt
      721 ggcagatatg aaaaaccggc ttcggataat cgaacagatg cagattgcag gaaagcaatg
      781 atctgacaga taccctcaca gtttcattcg gaaaaaattt tccagagagc gcaccctgct
      841 gtaaaagaag ctgcttaatt cagcagcttc ttttacatga aaaacagaaa ctggcttctg
      901 aaaacaaagg acttgagagt aatgaatgct tttgcagcgc tcgctgacga tactctccgg
      961 gacaaataga ataaattatg agcagattaa aaataatgtg ctgatgcata tccatggact
     1021 gtttcggtgt cggtgaagg
//
LOCUS       DAOQGJ010001945         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127458_length_1039_cov_2.616870, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001945 DAOQGJ010000000
VERSION     DAOQGJ010001945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127458_length_1039_cov_2.616870"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PK683_22780"
     CDS             complement(<1..728)
                     /locus_tag="PK683_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH11343.1"
                     /translation="MATDTSKYSEKLLKTRNIGISAHIDSGKTTLTERILFYTNRIHA
                     IHEVRGKDGVGAKMDSMDLEREKGITIQSAATYCQWKDYTINIIDTPGHVDFTIEVER
                     ALRVLDGAILVLCGVSGVQSQSITVDRQMRRYSVPRLAFINKLDRSGANPWRVIDQLK
                     EKLKHNAVAMQIPIGLEADHAGLVDLITMKAYYFEGANGTNIVEKEIPEELKELAEKK
                     REEMLDAISMWSDELMEAMLEGKVT"
     gene            914..>1039
                     /locus_tag="PK683_22785"
     CDS             914..>1039
                     /locus_tag="PK683_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNH11344.1"
                     /translation="MSYYRTVKGKKYDRQLLDLALESVSGKGDGRISLADAKKLIG"
BASE COUNT          245 a          227 c          210 g          357 t
ORIGIN      
        1 gtcaccttgc cttcgagcat tgcttccatg agttcatcac tccacatgga tattgcatcg
       61 agcatttctt ctcttttctt ttcagcaagt tctttcagtt cttcaggaat ttccttttct
      121 acaatgttcg ttccgtttgc gccttcaaaa tagtacgctt tcattgtgat cagatctaca
      181 agacctgcat ggtctgcttc gagtccgatt ggaatctgca ttgctacagc attgtgcttc
      241 agtttttctt tcagctgatc aatgactctc catggattcg ctcctgagcg gtcgagctta
      301 ttgatgaatg caagtctggg aacgctgtat cgtctcatct gtctgtctac tgtgatggac
      361 tgagactgaa ctccggatac accgcagagc accaagattg caccgtcgag aactcggaga
      421 gctctttcca cttcaattgt aaaatcgacg tgtccgggag tgtcaataat gttgattgta
      481 tagtctttcc attgacagta ggttgctgca gactggattg taattccttt ttctctttcg
      541 agatccatac tgtccatttt tgcaccgact ccgtcttttc cccgtacttc gtggatcgca
      601 tgaattctgt ttgtgtagaa taaaatcctt tcagttaaag tagtcttgcc tgagtcgata
      661 tgagctgata taccgatatt tctcgttttt aaaagttttt cactgtactt tgacgtgtca
      721 gttgccatga atttcctcta gattcccctg attctgttct ctcttcaggg cattcatttg
      781 accatgctag aaaacggcct tttccgaaaa agtttttttc tgtccgtttt ttgaatcaaa
      841 ttgcaaagag agtgctaact tgcttgcaaa aagaaggttt tcctgcatct tagatttttg
      901 aaggaaaatc actatgagtt attacaggac agtcaaaggt aaaaaatacg acaggcagct
      961 tctcgacttg gctctcgaat ctgtttcagg gaaaggtgac ggcaggattt ctcttgcaga
     1021 tgcaaaaaaa ctgattggt
//
LOCUS       DAOQGJ010001946         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127526_length_1039_cov_2.065041, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001946 DAOQGJ010000000
VERSION     DAOQGJ010001946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127526_length_1039_cov_2.065041"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PK683_22790"
     CDS             <1..696
                     /locus_tag="PK683_22790"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH11345.1"
                     /translation="AAPCTVIKIDAADAGNFLRHSPEVAISIARKLANRLVDLNKQIA
                     NLANSPFTASLTKLIEAERKKIENKEKVKEISFDTLSNLQVDFPPMYEIITQNEMLFN
                     LYILVKGEVEIIKNNKIIAIESEPGYYMGDVSILRKALPNATVRTRTECRMIQIPADR
                     VDSFLSHSPEVAISIARKIGERILSINNVMEKLKDDGKDDKKKTKKEDEGFQIDEKTS
                     AELEDLMKRLGIL"
     gene            complement(700..>1039)
                     /locus_tag="PK683_22795"
     CDS             complement(700..>1039)
                     /locus_tag="PK683_22795"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNH11346.1"
                     /translation="AMIADTPEKQGIGMMAVSELKDGEGMLYVFEKEEVPKFSMNQIR
                     LELEEIVISRDFTVAEIISTEPCTQKKCPVYRGSKKAEYSLEVPKGFSERKSIVPGNR
                     IILPSILFHR"
BASE COUNT          344 a          211 c          202 g          282 t
ORIGIN      
        1 gctgccccct gcactgtcat taaaatagat gcagcagatg caggaaattt tctgcgccac
       61 agtcctgaag tagcaatctc cattgctcga aaacttgcaa acagactggt tgacttgaat
      121 aagcagatcg ccaatcttgc aaactctccg ttcactgcaa gtctgacaaa actcattgaa
      181 gcagaaagaa agaagattga gaataaagag aaagttaagg aaatcagttt tgataccctc
      241 agcaatcttc aggttgattt tccccctatg tatgaaatca tcactcagaa tgaaatgctt
      301 ttcaatcttt atatccttgt caaaggagaa gtggaaatca taaaaaacaa taaaatcatt
      361 gccattgaat ctgagcccgg atactatatg ggagacgtat caattctgag aaaggcactt
      421 cccaatgcaa cggtcagaac aaggacggaa tgcagaatga tacagattcc tgcagacaga
      481 gtggacagtt ttctctccca ctctcctgaa gttgccatct ccattgcaag aaagataggc
      541 gaaagaattc tctctatcaa caatgtaatg gaaaaactga aagatgacgg gaaggatgat
      601 aagaaaaaga caaaaaaaga agatgaaggt tttcagatag acgaaaagac atctgcagaa
      661 ctggaagatc tgatgaaacg actgggtatt ctttagtgct catctgtgaa acagaattga
      721 aggaagaatt attcggtttc caggtacaat gctttttctt tcgctgaaac cctttggaac
      781 ttccagagaa tactccgcct tttttgagcc tctgtagaca ggacattttt tctgagtgca
      841 gggttccgta gaaataattt ctgcaactgt aaaatctctg ctgataacga tttcttcgag
      901 ttcaagacga atttgattca tggaaaattt tggaacttcc tctttctcaa atacataaag
      961 cattccttct ccgtctttca attctgaaac agccatcata ccgattccct gcttttcagg
     1021 agtatcagca atcattgcc
//
LOCUS       DAOQGJ010001947         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127657_length_1038_cov_3.181078, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001947 DAOQGJ010000000
VERSION     DAOQGJ010001947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127657_length_1038_cov_3.181078"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1038
                     /locus_tag="PK683_22800"
     CDS             <1..>1038
                     /locus_tag="PK683_22800"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH11347.1"
                     /translation="YSEPGKNTQNGASSESVSLLTAASSTSTCNDSAVEPPSSFGSQG
                     PEAFLSACSTDNMNRYRNLEIKFSDPMGQASVQGNFSIICNGSTLPGPGAGGTFIWKS
                     PQRLIFDPYRELSSNASCTITISNGAQTTEGKSLISYTKSFKAAHDYLITSSIQQGAV
                     NIVMGGQNDVTFNKSANLILNASFTNPTGAFDEIKKIVFNKMGNTDAQGNPLAGAKTI
                     CEDSCSSLGTPINLSTDSFMTGGMALTDGGNTYYFEITTKQLSKIQRYISFNYGNLNT
                     NPTGLINNVASGVLDQTQMMKLLERLIEVFAEQKFRVTNKTFNQFAQTPVSSARNTTK
                     CIDYKNGNFNY"
BASE COUNT          330 a          232 c          207 g          269 t
ORIGIN      
        1 atattctgaa cccggaaaaa atactcagaa cggagcatct tctgaatcag tgtctctttt
       61 gactgcagcc agcagtacca gcacctgtaa tgattctgct gtggaacctc caagctcgtt
      121 cggctcacag gggcctgaag catttttaag tgcatgcagc actgataata tgaacagata
      181 cagaaatctg gagattaagt tttctgaccc tatgggacag gcttctgttc aaggcaattt
      241 ttccattata tgcaacggct ccactcttcc tggtcctggc gcaggcggaa cgttcatctg
      301 gaaaagtcca caaaggctga tctttgatcc ttacagagaa ttaagttcaa atgccagctg
      361 cacaattaca atttccaacg gtgcacagac gactgaagga aaaagtctca taagttatac
      421 aaaatcattc aaagctgctc atgattacct gatcacatcg tctattcagc agggtgcagt
      481 caatattgtt atgggaggac agaatgatgt cactttcaat aaatctgcaa acctcatact
      541 gaatgcctca tttaccaatc cgacaggagc atttgatgaa atcaaaaaga ttgtattcaa
      601 caaaatggga aatactgatg cgcagggcaa ccctttggca ggggcaaaaa caatctgcga
      661 ggacagttgc tcttctttag gaactcctat caatctgagc actgattctt ttatgactgg
      721 cggaatggct cttactgacg gcgggaacac ctactatttc gaaatcacca ctaaacagct
      781 ttccaaaatt cagagataca tcagctttaa ttacgggaac ctgaacacca atccgacagg
      841 gctcatcaac aatgtagctt ccggtgttct ggatcagaca cagatgatga aacttctcga
      901 aagactgatt gaagtgtttg cagaacagaa attcagagtg acaaacaaaa cattcaacca
      961 gtttgcgcag actcccgttt cttctgcaag aaatacaacc aaatgtattg attacaagaa
     1021 tggaaacttc aactatat
//
LOCUS       DAOQGJ010001948         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127687_length_1038_cov_2.814852, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001948 DAOQGJ010000000
VERSION     DAOQGJ010001948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127687_length_1038_cov_2.814852"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..>1038)
                     /locus_tag="PK683_22805"
     CDS             complement(393..>1038)
                     /locus_tag="PK683_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11348.1"
                     /translation="HSLAKSFFGGLKQAMHGFNVQIGKLTGRKKTEADTLYSKNWGGG
                     LAEEYLKRSDLGRLNLKHMNTALDFMHNPEKTYSKSDFGRSDLGKMYSNQFEFMKGML
                     TNPGKTLQHSDFRRALGHIDKHLSDEYRTRERVREQFRIAGAYWYFKGWLWIPPPSSG
                     NGTTGGEGNHSPTDGGDGTTCIGTTSNNVNPANPFGCGNPSSPNSPDPSAPDAD"
BASE COUNT          279 a          237 c          181 g          341 t
ORIGIN      
        1 ttgccgtcac aaaaataatt aaataaaaca taactacccg tatcatctac tcaccttttt
       61 cttttcggtc ttctctgaag ccggacagaa tacctgactg cgacagtcga tttcacattg
      121 aaagtaatgt ccggttctgt ccgaaaaatg agacctttgt tgtgttgcac ttttgtaata
      181 aagagaggtt ccccaatatt gtttttgaaa gcactgcctg taacaataat caagttcagg
      241 acaagaattt ttcatcgttg ggttatggta tgaaattttc tcagactcac gctgggaata
      301 atattcgctc caataacgag ggctgctacc tgcacagtaa atcaagaaaa atatatgcat
      361 tgcgacattt attatttttt tattagaaat catcagtcgg catccggcgc agaaggatct
      421 ggactatttg ggctggatgg atttccgcaa ccaaaaggat ttgcaggatt tacattattt
      481 gaagttgtgc caatacatgt cgtaccatcc ccaccgtccg ttggagaatg atttccttct
      541 ccaccggtag tcccatttcc gctagatgga ggaggtatcc aaagccatcc tttaaaatac
      601 cagtatgctc ctgctattct gaactgttct cttactcttt ctcttgtgcg atattcatcg
      661 ctgaggtgct tgtcaatatg tccgagtgcc cttctgaaat cgctgtgctg aagcgtcttg
      721 ccaggatttg tcagcattcc tttcataaat tcaaattgat tactatacat tttccccaag
      781 tcacttcgac caaaatcgct tttcgaatat gttttttccg gattatgcat aaaatccaaa
      841 gctgtattca tatgtttcaa attaagtctt cctagatcac ttcgtttcaa atactcctct
      901 gcaagacctc cgccccaatt ttttgagtaa agagtatccg cttcagtctt ttttcttcca
      961 gtcagctttc ctatctgcac attaaacccg tgcatagcct gtttcaggcc gccaaaaaaa
     1021 ctttttgcta aactatgc
//
LOCUS       DAOQGJ010001949         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127859_length_1037_cov_4.310591, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001949 DAOQGJ010000000
VERSION     DAOQGJ010001949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127859_length_1037_cov_4.310591"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..834
                     /locus_tag="PK683_22810"
     CDS             190..834
                     /locus_tag="PK683_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11349.1"
                     /translation="MIYFRKFNLRQMAQNLLFWNSANHWKGQIMKIAIILLIVFLGCL
                     TAKNKELSQKENEYINIDDPAGLGRMKFKDPSDRKTFVYSRYQPTSFKELSGNSEQET
                     ASKGVKIFNAKKRLILNLPEYPLDGLNEDDLDQKNGYAFLNQSMQPMMHLYNSKLCLK
                     YNDQKFLFLFQSQLVPYLKREVKKGETVALYGLYATYNEFSNLHLILVNEFHKQ"
     gene            complement(850..>1037)
                     /locus_tag="PK683_22815"
     CDS             complement(850..>1037)
                     /locus_tag="PK683_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11350.1"
                     /translation="FQLEDGEIPEGENFIRLEMINDDFFAGNKWGLLLLPFTLALDAV
                     LWPFEIITLIMLSGWKT"
BASE COUNT          323 a          195 c          222 g          297 t
ORIGIN      
        1 tcctcggaag aaaaaactgg ctcttttccg actgccctga aggtgcggaa gcatcgctgt
       61 ctgaatgagt gaatatcaga cagcgatgct tccgcacttt cagggcagtc tgaaaaaagc
      121 cattttttcg aactggatct gtgcgaaaag attctttaag cttgattttt cagcctatag
      181 gaatgatata tgatatattt caggaaattc aatctgcggc agatggctca gaacctgctg
      241 ttttggaatt ctgcaaatca ctggaaggga cagatcatga aaattgcaat cattttactc
      301 attgttttcc ttggatgtct gactgcaaaa aataaggaat tgagtcagaa agaaaatgaa
      361 tatataaaca ttgatgatcc tgctggtctg ggtcgtatga agttcaagga tccctctgac
      421 agaaaaacat ttgtatattc cagataccag ccgacttcat tcaaggaact ttccgggaac
      481 agcgagcagg agactgcttc aaaaggggta aagattttca atgcaaagaa aagactgatc
      541 ttgaatctgc ctgagtatcc gttggacggt ctgaatgagg atgatctgga tcagaaaaac
      601 ggatatgctt ttttgaatca gtccatgcag ccgatgatgc atttatataa ttctaaatta
      661 tgtttaaaat ataatgacca gaaatttctt tttctgtttc agtcccagct cgtgccgtat
      721 ttaaaaaggg aagtgaagaa aggggaaaca gttgcgttgt atggactgta tgcaacttac
      781 aatgagtttt caaatcttca tctgattctg gtgaatgaat ttcataagca gtaagacagg
      841 ttcgctttcc tatgttttcc agccggacag cataatgaga gtgatgattt caaacggcca
      901 gaggactgca tctaaagcca aagtgaacgg cagtaaaagc agtccccatt tattgcctgc
      961 aaaaaaatca tcattgatca tctccagccg gataaaattt tctccttcag gaatttcacc
     1021 gtcctccagc tgaaaca
//
LOCUS       DAOQGJ010001950         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127861_length_1037_cov_4.180244, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001950 DAOQGJ010000000
VERSION     DAOQGJ010001950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127861_length_1037_cov_4.180244"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1037)
                     /locus_tag="PK683_22820"
     CDS             complement(<1..>1037)
                     /locus_tag="PK683_22820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11351.1"
                     /translation="VYSLSEGAWIQHRKVRLTSYKIEVIGEDAIFGHLHGGVKVEDPE
                     NRITFKAAKADYDKIQELITMQGRPILQYYNSSNKLTKITAPYMKRYLLENKTSLEGG
                     VIMEDGEYTVISETAVFYEKEDKLVMENHPFVFGKETFITGEKAVYNTDTKTTSIEKD
                     TLLARLSFEVKEDSVPGAKKAETESEKKEKVRTVTVMTGDKMESYSGTETDSYAGFFG
                     NARIFREDYEYSGAYLKAKGKDYKNLEAKDQVVFTDKVNNVRMSGKLFEHFEDEKYTH
                     ITEEPKIEFLSKEGEVKSTLRCVELERFGERKEMVARGNVVIESEESIIRGDYGTYFE
                     EEKKLVVEGNPT"
BASE COUNT          243 a          252 c          165 g          377 t
ORIGIN      
        1 gtgggatttc cttccacaac cagctttttt tcctcttcga aataggttcc ataatcaccg
       61 cgaatgattg actcttccga ttcaatgacc acatttccgc gagctaccat ttcctttctt
      121 tctccaaacc gttccagttc cacacatctc aaagtcgatt ttacctcgcc ctctttcgac
      181 agaaattcta tctttggctc ttctgttata tgagtgtatt tttcatcctc aaaatgttca
      241 aaaagcttcc ctgacattcg tacattgttc actttgtcgg taaataccac ctgatccttg
      301 gcttcaagat ttttatagtc ttttcccttt gccttcagat atgctccgct gtattcataa
      361 tcctctctga aaattctggc attcccgaaa aatcctgcat aagaatcagt ttcagttcct
      421 gaataagatt ccattttatc accggtcatt actgtaactg ttcttacttt ttcctttttt
      481 tccgattcag tctcagcctt ttttgcgcct ggaacagagt cttccttcac ttcaaaagat
      541 aacctggcaa gcagagtatc tttctctata gaagtagtct ttgtatccgt attgtacaca
      601 gccttttctc ctgtaatgaa agtttccttt ccgaaaacaa atggatgatt ttccattaca
      661 agtttgtctt ccttttcata aaaaactgct gtctccgata tgacagtata ctctccgtcc
      721 tccataatga ctcctccttc gagagatgtc ttattttcga gaagatatct tttcatatat
      781 ggggcagtga ttttggtcag cttgttcgaa gaattataat actgaaggat cggtcttccc
      841 tgcattgtga tcagttcctg aatcttgtcg taatctgcct ttgctgcctt gaatgtaatt
      901 ctgttttccg gatcttctac tttgactcct ccgtgcagat gtccaaagat agcgtcctcg
      961 ccgatcactt caattttata ggaagtgagt ctgacttttc tgtgctgaat ccaggctcct
     1021 tctgaaagag agtagac
//
LOCUS       DAOQGJ010001951         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127946_length_1037_cov_2.456212, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001951 DAOQGJ010000000
VERSION     DAOQGJ010001951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127946_length_1037_cov_2.456212"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..>1037
                     /locus_tag="PK683_22825"
     CDS             319..>1037
                     /locus_tag="PK683_22825"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNH11352.1"
                     /translation="MNEEELIKRIQILEEQMEAINRRPSYTKWISVIRKYKLLLFSLL
                     FIPFGILLAATVTKPHIFTSGEKVSASKVNENFDTLYAKVNENTPTGTILAFAGTNVP
                     SGWLLCNGSAVSRTTYDELFKTIGTAFGNGDGSTTFHIPDFRGRFLRGLDGTAGNDPD
                     SGSSSNSGADGKRFALNGGNTGNAIGSYQIDGIKSHQHVMNFGSTGGGIAMWFSSVTS
                     GAGSPVNYTDNFGGNETRPKNV"
BASE COUNT          326 a          214 c          217 g          280 t
ORIGIN      
        1 ggagtcctct gatgcatggt ttcccgcttc ttttcccagg ttctactgtt attgtttttt
       61 tccaatccat actgctaatt taaatttttt agaaaaggag tcaaggtttt cttggatgaa
      121 cgtggacagc attatgatca gactttcctc cattccaaat gaaataacct catcatcctc
      181 gaggattaga actccctgca aagacaagga ttccaaaggg acgaagtccc tttggccctc
      241 cgggaggatc acactcagct tgcgaagcaa gctgcagata tttactgtat tgcatttata
      301 aaaaccaaag gagacgctat gaatgaagaa gaactgatca aaagaatcca gattctggaa
      361 gaacagatgg aagcgataaa tcgccgcccg tcatatacaa aatggatctc tgttatccga
      421 aaatataaac tgcttttatt ttcattatta tttatcccat ttggaatcct tcttgcggca
      481 accgtcacaa aaccgcacat cttcacctca ggagaaaagg tttctgcatc aaaggtaaat
      541 gagaattttg acacactgta tgcgaaagtg aatgaaaata cacccacagg aacaatcctt
      601 gcttttgcag gaacaaatgt tccttccgga tggctcttat gcaacggctc agcggtaagt
      661 cgaacaacct acgatgaact ttttaaaaca ataggaacag catttggaaa cggtgacggt
      721 tctacaacat ttcatatccc cgatttcaga ggcagatttc tgagaggcct ggatggaaca
      781 gctggcaatg atccggacag tggatcatct tctaattcag gagctgacgg caaaagattt
      841 gcactgaatg gaggaaatac tggaaatgca atcggcagtt atcagattga tggtattaaa
      901 agtcatcaac atgtaatgaa ttttggttca accggaggcg gaatagctat gtggttcagt
      961 tcagtaacat ccggtgcagg aagtccagtt aattatacag ataattttgg aggcaacgaa
     1021 acccgtccca aaaacgt
//
LOCUS       DAOQGJ010001952         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127970_length_1037_cov_2.266802, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001952 DAOQGJ010000000
VERSION     DAOQGJ010001952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127970_length_1037_cov_2.266802"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..790)
                     /locus_tag="PK683_22830"
     CDS             complement(224..790)
                     /locus_tag="PK683_22830"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH11353.1"
                     /translation="MNYLFTMSAVLEKSIPPQILFSVEMYHTMYEQGLIPEKSELIEG
                     VVYSKMTKNPIHSDTVSKILILLIRSHSGKYRILSENPISLQNSEPEPDIAVVAEGDY
                     SKEHPKKALLVIEVSNTTLNFDRKKASVYAKGNIPEFLIINLIDYKVEIYRSPKEGIY
                     TEIKILSKEEIFSSVSVEGLQFSLSDFI"
BASE COUNT          345 a          176 c          208 g          308 t
ORIGIN      
        1 gaagtgatga aattgatgat gggacatata ctcaggacag gaatatagtg aatatatact
       61 caaacggcga cagaaaaacc tatcagtttt ccctctcaaa agacgggatt gaactgacct
      121 atgaggaata ttctgcagtc ttcagagatg aaaaatatcc ctggaaatga acaccataca
      181 cagacagaaa atggaatcag gaacctgtat ttacaaaaat atattagata aaatcagaca
      241 gagaaaactg aagtccttcc acggatactg aagagaatat ctcttcttta gagagaattt
      301 taatttctgt ataaatccct tctttgggag aacggtaaat ttcaacttta tagtcgatca
      361 ggttgataat gagaaattca ggaatattcc ctttggcata taccgaggct ttttttctgt
      421 caaagttcaa tgtggtattg gatacctcaa ttaccaggag ggctttcttc ggatgttcct
      481 tggaataatc cccttctgca accactgcaa tatccggttc cggttccgag ttctgaaggg
      541 aaattggatt ttcagaaagg attctgtact ttccggagtg ggatcgaatc aaaaggatta
      601 aaatttttga aacagtatca gaatgaatcg gatttttggt cattttagag tatacgactc
      661 cttctatcag ttcagatttt tccggaatca ggccctgttc atacatagtg tggtacattt
      721 ctactgaaaa cagaatttga ggtggaatag atttttccag aaccgcagac atagtgaaaa
      781 gataattcac ggattgaatc agtcaagata aatttgcctg aactgggagc ggattttcca
      841 aaggattttt tcaatggcag cattcacatt gacttctctg aaggacagaa cagcagaact
      901 ggtcttgtcc attggggagt cccgatattg aaaatatacg gagaaaagat taccgggaaa
      961 agataaaaag ttatattatc agaaaaaaat ataaatctga gcttattatt cctgttatac
     1021 tcactcatct ttctttg
//
LOCUS       DAOQGJ010001953         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_127971_length_1037_cov_2.244399, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001953 DAOQGJ010000000
VERSION     DAOQGJ010001953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127971_length_1037_cov_2.244399"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..>1037
                     /locus_tag="PK683_22835"
     CDS             127..>1037
                     /locus_tag="PK683_22835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11354.1"
                     /translation="MNLFFFLVWLHSVHLYLKTEAEPNAEWMIFTSILAGTARYESIL
                     LIIPSLFLILTAVFRKNFRLELLTWRTVLSVILFFPLLWQRMVSSSLLNAGDSNKDPI
                     SFSYLFPNILNLADFFRNLVPGTFPSSSALFFLCIAGIGVLLWNFRKSEYSSEEKNTI
                     LTAFMGLCLVYSVRLAYYFGDLRLPWVHRLSLEAAFVLCIPAAVFLKYLFVDIFKKNI
                     IYYTIIFLIAMQNLSTAAGNQSIRTITTHREFINNLNFLKGFPKEGTLVISGRPGMYV
                     SHGINAVSFDTVLTKTNELENWMNNKIA"
BASE COUNT          273 a          215 c          197 g          352 t
ORIGIN      
        1 tttatttccg tatttcgtct tgctgaaaaa tttacagagt ctgaggaaat cagaatcggt
       61 ctgccgattc ttgtactctc gtttccgatc actgccattt gctttcgttc cggcggattt
      121 gatgcattga atcttttctt ttttctggtc tggctccatt ctgttcatct ttatctgaaa
      181 actgaggcag agccaaatgc agaatggatg atatttactt ccatccttgc aggaactgcc
      241 agatatgaaa gcatacttct catcattcct tctctttttc tgattctgac tgcagtgttc
      301 agaaaaaatt tccgtctgga attactgacc tggagaacgg tattgtccgt gattcttttc
      361 tttcccctgc tctggcagag aatggtttca tcgagcttac tgaatgcagg ggattccaac
      421 aaggatccaa tttcattttc gtatcttttt ccaaatatac tgaaccttgc agattttttc
      481 agaaatcttg tgccgggaac gtttccttcc tcttctgccc ttttctttct ctgcattgca
      541 ggaatcggag tgcttctctg gaatttcaga aaatcagagt attccagtga agagaaaaat
      601 acaatcctga ctgcatttat gggactctgt cttgtctatt cagtaagact tgcctattat
      661 ttcggagatc tcagacttcc ctgggttcac aggctctctc tggaagcagc atttgtcctg
      721 tgcattccag ccgctgtttt tctgaaatac ttgtttgttg atatattcaa aaaaaatata
      781 atatactata ctataatatt cctgattgca atgcagaatc tttctactgc ggcaggaaat
      841 cagagcatca gaacaattac gacccatagg gaattcataa ataatctgaa ctttctcaag
      901 ggatttccca aagaaggaac acttgtgatt tcgggcaggc cgggaatgta tgtttcacac
      961 ggtataaatg cagtttcatt tgatacggtt ctgacaaaaa caaatgaact tgaaaactgg
     1021 atgaataata agattgc
//
LOCUS       DAOQGJ010001954         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_128212_length_1036_cov_2.240571, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001954 DAOQGJ010000000
VERSION     DAOQGJ010001954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128212_length_1036_cov_2.240571"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..>1036)
                     /locus_tag="PK683_22840"
     CDS             complement(8..>1036)
                     /locus_tag="PK683_22840"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNH11355.1"
                     /translation="NAQFIGGEEVAKIEKSLAEYAETKFSVSCANGTDAIQLALRAVG
                     IGRGKKVLLPNSTFWATFEAVVNVGADPYTVDTDLVDLQMDFSVFEKAADEIKPDAAI
                     IVHLYGWGSASLLKFREFCRSRNIPLIEDGAQCFGVRINGESIYKNAYISTTSFYPAK
                     VLGAAGDGGAVFTNDAGLADTVRKLANHGRTSHYGHGFAGWNSRLDSLQAAFLNLSME
                     VFSSRLESRKKIAKKYQADLKTRGYNVITPPSIYEENGYCNAMLLPNSERTKLEALLK
                     ENGIGFANIYPGAMSDQEGSRGFLVKKFGGDNAREISSTVLNLPLFAYMTDSEYSEVL
                     SVIDRFKK"
BASE COUNT          279 a          253 c          204 g          300 t
ORIGIN      
        1 caagcgatta tttcttaaat ctgtctatga cactcagaac ttctgagtat tcagaatcag
       61 tcatgtatgc aaaaagagga agattcagga cagtgctgct gatttctctc gcattgtctc
      121 cgccgaattt ttttaccaga aatcctctgc ttccttcctg atcggacatc gctcccggat
      181 atatattggc aaagccgatt ccgttttcct taagaagagc ctcaagtttt gttctttcag
      241 aattaggaag aagcatcgca ttacagtatc cgttttcctc gtaaatcgaa ggcggcgtaa
      301 ttacattgta tcctctagtc ttaagatcgg cctgatattt ctttgcgatt ttttttcttg
      361 attcgagtct cgatgaaaac acttccatgc tcagattcag aaatgcagcc tgaagcgaat
      421 ccagtcttga attccagcct gcaaaaccat gtccgtaatg agaggtcctt ccatgattgg
      481 ctagttttct cacagtgtca gccagacctg catcgtttgt gaagactgcc cctccgtctc
      541 cggctgcacc gagcaccttt gcaggataaa aggatgtagt ggaaatataa gcatttttat
      601 aaatggattc accgtttatc ctgactccga agcactgagc accgtcttca atcagaggaa
      661 tattcctgct cctgcagaat tctctgaact tgagaagtga ggcagatccc catccgtaca
      721 gatgaacaat gattgccgca tccggtttta tttcatcggc agccttttcg aagacagaaa
      781 aatccatctg aagatcgacg agatcagtgt ccacagtata aggatcagca cctacattca
      841 ctacagcttc aaatgttgcc cagaaagtcg aattaggcag aagaaccttt ttgcctctgc
      901 cgattccgac agcacgaaga gccagctgaa tagcatcagt tccgtttgca caggaaacag
      961 agaactttgt ttcagcatac tctgccagac ttttctcaat ctttgcaact tcctctccgc
     1021 cgataaactg agcatt
//
LOCUS       DAOQGJ010001955         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_128400_length_1035_cov_2.801020, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001955 DAOQGJ010000000
VERSION     DAOQGJ010001955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128400_length_1035_cov_2.801020"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..>1035
                     /locus_tag="PK683_22845"
     CDS             109..>1035
                     /locus_tag="PK683_22845"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNH11356.1"
                     /translation="MEELNEAEKKIKDALDGMSNEYTGKVKTVNQALGKKFAYKREFP
                     EFDSLLSDESKQSQILEFRKKTMNKKIIYILSAAAVLLGGVFAAITFKDGSGKGDEKN
                     KVSQEAKVTFVSGNVTVKTSDGKETKAAVGTVIPITASIITANKSMADVQFGQGNTIR
                     IKANSEASIKSIVYGAVSEEEVFLKKGVIMANVSKKKQNDTFNIMTPTVIAGVRGTVF
                     QVMYNPQNPSESTVISVGEGSIAVTPQKDGKPLSPEPKEILDSDDQAIEKGIGGEIVK
                     KKISHPEMRKILDDNFDIDDEKELLQSTGRSEI"
BASE COUNT          374 a          178 c          231 g          252 t
ORIGIN      
        1 ctgtaatttt gtttataaaa aaaattattt taaaggactt taggaattac tctgagctga
       61 tcaggcttgc cgaagaaaac gaaatattcc cataagggcg gcgtgaatat ggaagagtta
      121 aacgaagcag aaaaaaaaat caaagatgca ttggatggaa tgtccaatga atacactgga
      181 aaggtaaaga cagtcaatca ggctttagga aaaaaatttg catacaaaag agaatttcct
      241 gagtttgatt cgttattatc tgatgagagc aaacagtcac aaattttaga attcaggaag
      301 aaaacaatga ataaaaaaat tatttacata ctcagtgcag cggcagtcct tttgggcgga
      361 gtattcgctg cgataacttt caaggacggt tccggaaaag gcgatgagaa aaacaaagtc
      421 tctcaggaag caaaagttac tttcgtcagc ggaaatgtca ctgtgaaaac ttctgacggc
      481 aaggaaacaa aagcagctgt cggaactgtc attcctatca ctgcaagcat catcactgca
      541 aataaatcta tggcagatgt gcagttcggg cagggaaaca caatcaggat caaggcaaat
      601 tctgaagcat ccatcaagtc tattgtatat ggcgcagttt ctgaagaaga agtatttctg
      661 aaaaaaggtg tcattatggc caacgtcagc aagaagaagc agaatgatac tttcaacata
      721 atgactccta cagtcattgc gggtgtaagg ggaactgtgt ttcaggttat gtataatcct
      781 cagaatcctt cagagtccac tgtcatctcg gtaggagaag gaagcatagc cgtaactcct
      841 caaaaagacg gaaagcctct gagtcctgaa cctaaagaaa ttctggactc agatgaccag
      901 gcgattgaaa agggtatcgg cggagaaatc gtaaagaaga aaatcagtca tcctgaaatg
      961 cgcaagattc tggatgacaa ctttgacatt gatgatgaaa aagaacttct tcaatctact
     1021 ggaagatctg aaatt
//
LOCUS       DAOQGJ010001956         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_128433_length_1035_cov_2.401020, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001956 DAOQGJ010000000
VERSION     DAOQGJ010001956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128433_length_1035_cov_2.401020"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /gene="hslV"
                     /locus_tag="PK683_22850"
     CDS             <1..349
                     /gene="hslV"
                     /locus_tag="PK683_22850"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNH11357.1"
                     /translation="LFEKKVQTYNGNLSRSVVELAKDWRTDRMLRKLEALLIVADRTE
                     SFLVSGTGEVISPDDGILAIGSGGNYALSAARALYKHSSLNAAEIVKEGLEIAADICV
                     YTNHNITVEELAI"
     gene            346..>1035
                     /locus_tag="PK683_22855"
     CDS             346..>1035
                     /locus_tag="PK683_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH11358.1"
                     /translation="MNELQPLENREEKSDTGFEFDLTPSQIVTELDQHIIGQKNAKKA
                     VAIALRNRTRRRKLEPSIREEIYPKNILMIGPTGVGKTEIARRLAKLCRAPFLKVEAT
                     KYTEVGYVGRDVESMIRDLMSVSLNLVKAEFRKKVEEDARKKAEDLVLDILIPPPPAP
                     SGAGFQTDFISAEEEERKKSYQSTREIFRSRLRNGKFDSQEIEIELSSPNPGNMPSMQ
                     VFGAGNLEEMDS"
BASE COUNT          335 a          202 c          266 g          232 t
ORIGIN      
        1 gctttttgaa aaaaaagttc agacatacaa cgggaatctt tcgagaagcg tagtggaact
       61 cgcaaaagac tggaggacgg acagaatgct cagaaagctg gaagctcttc tgattgtggc
      121 ggacagaact gaatccttcc ttgtttccgg gacgggagaa gtgatttcac cggatgacgg
      181 gattctggcc atcggttccg gcgggaatta tgcactttca gcagccaggg cattgtacaa
      241 gcattcctct ctgaatgcag ctgaaatcgt aaaagaaggg ctggaaatag cagccgatat
      301 ctgtgtttat acaaatcata acataacagt agaagagctt gctatatgaa tgaactgcag
      361 ccattggaaa acagggaaga aaagtcggat acaggttttg aatttgatct gactccttct
      421 cagatcgtaa ctgaactcga tcagcacatt atcggtcaga aaaatgcaaa aaaagcagtg
      481 gcaattgcac tgcgaaacag gacaagacgg agaaaactgg aaccgtccat tcgtgaagag
      541 atttatccaa aaaatatact gatgatcgga cctactggag taggaaagac agagatagcc
      601 agaagactgg ctaaactctg cagagcgccc tttctgaaag tggaggctac aaaatacact
      661 gaagtagggt atgtgggacg ggatgtggag tcaatgatcc gcgatctgat gagtgtatct
      721 ctgaacttag taaaggcaga attcagaaag aaagttgaag aggatgcacg taaaaaagca
      781 gaggatcttg tactcgatat tctcattcct ccgcctcccg ctccgtccgg agcaggtttt
      841 cagaccgatt tcatcagtgc agaagaggaa gaaagaaaaa aaagctacca gtctaccagg
      901 gagattttcc ggtcgaggct tcgaaacggt aaatttgatt cccaggagat tgaaatcgaa
      961 ttgagttcac cgaatcccgg aaatatgcca tccatgcagg tattcggagc aggcaatctg
     1021 gaggaaatgg attcc
//
LOCUS       DAOQGJ010001957         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_128600_length_1034_cov_3.083759, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001957 DAOQGJ010000000
VERSION     DAOQGJ010001957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128600_length_1034_cov_3.083759"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..657
                     /locus_tag="PK683_22860"
     CDS             358..657
                     /locus_tag="PK683_22860"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH11359.1"
                     /translation="MSRKIFPSNLSDFFKALGNDKRQEILLNVFSDFKEHNIGEVAKK
                     AGIAQSTASEHLSFLKRAGILTSEKVEKEVFYRVDRESVLSALDEIRTVLSCCMK"
     gene            716..>1034
                     /locus_tag="PK683_22865"
     CDS             716..>1034
                     /locus_tag="PK683_22865"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH11360.1"
                     /translation="MIEAIEVSADRLRKAYDRRSGIYAATVAKAEWPNHLKTMEKIRI
                     EPSDRILEVAVGPGFSLVELLKRSGGENNVFGVDMSAGMLDLARKRVSAAGFSNVDLR
                     QADA"
BASE COUNT          314 a          181 c          254 g          285 t
ORIGIN      
        1 cactgctgtt ctgtctcaga atggaaaaat gaatactgaa aagaaggact tttaacgagc
       61 ctcggactga tttttaatac acctgattat ctttcagaag ataaattgtg catttataaa
      121 attccagata aatgtacagc ctgtttgact gccctcatcg ccggcagtcg acagcattgg
      181 cctgcctgca gagtcaggtt agcgatcttg aaagccgctg ttttaaatat ccgttgaaag
      241 ctgtcaaaag acagatctat gcatctgcgc agttccggca tatgcataaa aaacttttaa
      301 aaatcgattg acttttttgg ggtttgaatt agtgttggaa tatcgggaaa tgacgaaatg
      361 agcaggaaga tatttccatc caatctctca gattttttta aggctcttgg aaatgacaag
      421 agacaggaaa ttcttctgaa tgttttttct gatttcaagg agcacaatat cggagaggtg
      481 gcaaagaaag caggaatcgc gcagtctact gcttcagaac atctttcttt cctgaaaagg
      541 gcgggcattt tgacatcgga gaaagtggaa aaagaagttt tttacagagt ggacagagag
      601 tcggttcttt ctgcgcttga tgagatcagg acagttttaa gctgctgcat gaaataattt
      661 ttttgcatta tatttcgtta tttcccgaaa taactaagtt taagtgaggg gcgccgtgat
      721 agaagcgatt gaagtttcag cggacaggct cagaaaggcc tatgacagaa gaagcggaat
      781 atatgccgca acagtggcaa aggccgaatg gccgaaccat ctgaaaacta tggaaaaaat
      841 cagaattgaa ccttctgaca gaattttaga agttgctgta ggccctgggt tcagcctcgt
      901 tgagcttttg aaaagatccg gcggagaaaa caatgtattc ggagtggata tgtctgcagg
      961 aatgcttgat cttgcaagga aaagagtttc tgcagcggga ttcagcaatg tggatctcag
     1021 gcaggcggat gcag
//
LOCUS       DAOQGJ010001958         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_128653_length_1034_cov_2.502554, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001958 DAOQGJ010000000
VERSION     DAOQGJ010001958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128653_length_1034_cov_2.502554"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1034)
                     /locus_tag="PK683_22870"
     CDS             complement(<1..>1034)
                     /locus_tag="PK683_22870"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNH11361.1"
                     /translation="PRAFVLDGFAAIIIFGTALLSLPKAHTAEIPLIDIFFTVISATC
                     VTGLSTIDISVSLSFTGQIILMFLIQIGGLGLITLTSFFSIYLAGQASVNDKLLMKDI
                     LSEETLGRVAKLVKDITMQTAIIESAGAAMLYIHFPDNTKYSFFERIFISVFHSVSAF
                     CNAGFSLFSRNLGEDFIMFEKKFLMVIMILIVLGGIGFPVIAQVWTKFMTEEGRTMKL
                     NTSSRLAITVSFIFILFGGISYFFLEKQNTLSGLDFDDSLFQAFFYSVSTRTAGFNTL
                     DMAKLGMPMTFLSLFLMWVGASPNSTGGGIKTTAFAISLMQIFDFVRGKDRVEIFRKE
                     IHPSSLYRAS"
BASE COUNT          321 a          217 c          225 g          271 t
ORIGIN      
        1 cgatgcacgg tacagggaac tgggatggat ttcctttcgg aaaatttcca ctctgtcctt
       61 gcctctcaca aaatcaaata tctgcatcag actgattgca aatgcagtgg tcttgatgcc
      121 tccccccgtt gaattgggag atgcacctac ccacataaga aacagtgaga gaaaagtcat
      181 cggcattccc agttttgcca tatcaagagt attgaacccg gctgtccttg tgctgacaga
      241 gtagaaaaaa gcctggaaca gtgaatcatc gaaatccaga ccggaaagag tgttctgttt
      301 ttccagaaag aaataggata ttccgccgaa cagtatgaaa atgaaactta cagtaatcgc
      361 aagtcggctg gatgtattca gtttcattgt tctgccttct tccgtcataa attttgtcca
      421 cacctgtgca atgactggaa agcctattcc tccaagcaca atcagaatca taattaccat
      481 cagaaacttt ttttcaaaca taataaaatc ttctcccagg ttcctgctga aaagagagaa
      541 gcctgcattg cagaatgcag aaactgaatg aaatacagaa ataaaaattc tttcaaaaaa
      601 agaatatttt gtattgtcag ggaaatgaat ataaagcatt gctgcgccgg cagactcaat
      661 gatcgcagtc tgcattgtga tgtctttgac aagttttgcc actctgccga gagtttcttc
      721 gctgagtatg tccttcatta gcagtttgtc attcactgag gcctgaccgg ccagatagat
      781 ggaaaaaaaa ctggtgagtg taatcagtcc aagccctcct atctgaatga gaaacataag
      841 tatgatttga ccagtgaagg aaagtgaaac agagatgtct attgttgaaa gccctgtcac
      901 acaggttgcg ctgatgactg taaagaatat gtctatgagc ggtatttccg ccgtatgtgc
      961 ttttggaaga gaaagcaggg cagtcccgaa aatgatgatc gcggcaaaac cgtcaagtac
     1021 gaaagcccgc ggtc
//
LOCUS       DAOQGJ010001959         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_128804_length_1033_cov_4.105317, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001959 DAOQGJ010000000
VERSION     DAOQGJ010001959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128804_length_1033_cov_4.105317"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PK683_22875"
     CDS             complement(<1..858)
                     /locus_tag="PK683_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11362.1"
                     /translation="MLIPPSAGEKTWRWNIAEIRSVNITGNVVDLMAGKIRNLPSDSS
                     DLLLLASCIGIKFDFEILASVSGLSLRAVSEKMKKIADEGMITAEGYSAKFSHDKVRE
                     AAYSLIPSEKKMQNHYILGKKILETSPPEMLEENIFTILAQLNPSRHLIESEEEKQRL
                     IVLNLQAGRKAKASLAYDRALIFLKTGSEFLTEKKWLENYRLCMDVLTELAECEYLAG
                     NFRAADILYERILPKAADIHDKISIIHIQLRQKASEFKPDEAFVIGFSVLKELGIEVP
                     DIFSPEEVQS"
BASE COUNT          292 a          238 c          203 g          300 t
ORIGIN      
        1 agactgaacc tcttcaggac tgaagatatc gggaacttcg attcccagct ctttcagaac
       61 ggaaaacccg atgacgaagg cctcatcagg cttaaattca ctggccttct gtctgagctg
      121 tatatgaata atactgattt tatcatgaat gtctgcagcc ttgggaagaa ttctttcata
      181 aagaatgtcc gcagccctga aattgccggc aagatactcg cactcggcaa gttcagtcag
      241 aacatccatg cacagcctgt aattctccag ccattttttt tcggtcagaa attccgatcc
      301 ggttttcaga aagatcaatg ccctgtcata agcaagcgat gcctttgcct ttcttcctgc
      361 ctgaagattc agtacaataa gtctttgttt ttcctcttcg gattcaatca gatgcctgct
      421 cggattcagc tgcgccaata tcgtaaaaat attttcttca agcatttcgg gaggagaagt
      481 ttccaggatt tttttaccta aaatataatg attctgcatt tttttttcag agggaataag
      541 agagtatgct gcctccctta ccttgtcatg agaaaattta gcactgtacc cttctgctgt
      601 aatcattcct tcgtcagcaa tttttttcat tttctcactg actgccctga gagacagtcc
      661 tgaaacagag gcaagtatct caaaatcaaa ctttatgcct atgcatgatg ccagaagcag
      721 gagatcggag ctgtccgaag gaagatttcg gatcttgcct gccatcagat caaccacatt
      781 ccccgtaatg ttcactgatc tgatctctgc aatattccat ctccaggttt tttctcccgc
      841 agaaggagga ataagcattc cctccggata aatttgaaga agccggcaga gaaataaatc
      901 tcgaagaaag aaaggctgat aatttcacag tcggactgag ttttcatctg aaagaacttc
      961 ctgaaaaaaa cagctcatac ggactcttcc ttgaaagaat cggaatcaac tgggcagtgt
     1021 atctgaacgg tca
//
LOCUS       DAOQGJ010001960         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_128818_length_1033_cov_3.662577, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001960 DAOQGJ010000000
VERSION     DAOQGJ010001960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128818_length_1033_cov_3.662577"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..>1033
                     /locus_tag="PK683_22880"
     CDS             360..>1033
                     /locus_tag="PK683_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH11363.1"
                     /translation="MSHPIIEMRGVHKAFTRKILKGMNMTVRKGETMVILGPSGTGKS
                     VTLKHITGLVQPDQGDCFIDGESISFSSEKIRQRLRARMGVLFQSGALINWLSVYDNV
                     ALPLREHKLASGAELDRIVMERLKWLDLVHAKDNLPGDISGGMKKRAGIARALTTNPE
                     ILLYDEPTSGLDPVMSNVFNELILRLQKELSVTSIVVTHDMQSAYMIADRISFIYEGE
                     VILCGTP"
BASE COUNT          306 a          191 c          251 g          285 t
ORIGIN      
        1 taaatgaatc tgtaaactca ctgccaatga gagagcttgc agatgtcttg aactgtgttt
       61 agtaaagcat atccagaatg tattaaggat cggccatatt cagatgtcaa ctaatgcatt
      121 aaggcttcct gtcagtgaag tttttgaggt aggaactcct ttttagtatg agacataatc
      181 tgaagtcagc tctcacgtta gaatgtggga actccatcgg taaatgagat atggtttctc
      241 tggaaaagcg gggacttctg cagaagaagg tttgtctgaa tagccctggc aattttgata
      301 aattcatagt cccaaaaagg aaatgacagg atacagtaaa ctctaaaact gtactggtta
      361 tgagtcatcc tatcatcgaa atgcgcggtg ttcacaaggc tttcactaga aaaattctga
      421 aaggaatgaa tatgactgta cggaagggtg agacaatggt gattctggga ccttccggaa
      481 cagggaaatc tgttacactc aagcatatta caggacttgt tcagccagat cagggcgact
      541 gttttattga cggggaatcc atttcgtttt ccagtgaaaa aataagacag agacttcggg
      601 caagaatggg tgtccttttt cagtccggag cgctgatcaa ctggctttca gtatatgaca
      661 atgttgctct ccctctcaga gaacataagc ttgcatcggg tgcggaactt gaccgtatcg
      721 ttatggaaag actgaaatgg cttgacttgg tacacgctaa ggacaatctt ccaggggaca
      781 tcagcggggg aatgaaaaaa agagcaggca tcgcaagggc tctgaccaca aatcctgaaa
      841 tccttcttta tgatgagcct acttcaggtc ttgatccagt aatgtcgaat gtgtttaatg
      901 aacttatatt gcgtcttcag aaggaactct ccgtcacgtc gatcgtagtt acgcatgata
      961 tgcagagtgc gtatatgatt gcagacagaa tcagttttat ttatgaaggg gaggtgatct
     1021 tatgcggaac gcc
//
LOCUS       DAOQGJ010001961         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_129057_length_1032_cov_3.462641, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001961 DAOQGJ010000000
VERSION     DAOQGJ010001961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129057_length_1032_cov_3.462641"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(578..>1032)
                     /locus_tag="PK683_22885"
     CDS             complement(578..>1032)
                     /locus_tag="PK683_22885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11364.1"
                     /translation="IDSLTHDSFVIQLFELKTEMRNRLEKVLSVFSHSSSRDGYRAYP
                     SKDHIVYYDYPLDDELQQKIVQRLNYAVSDETLKRQMDLEEEILMELQDLERKIENQE
                     KTIEEKEKTIEESRKTIEESRKTIEEKEKTIEEKDKLIEELMKKLKDK"
BASE COUNT          247 a          213 c          202 g          370 t
ORIGIN      
        1 gtaagcgggg aaaatgtcat atatccaaga taactggatg tatcaattgt agactgtttg
       61 aatttcagtg tcactgatcc gcctgctgat gtaggttctg caaccacatt cactgatgca
      121 tcgcaggcac ttggagcctt gagcgaattc agtgcaagaa gaccgatcag tgttttggag
      181 ttgtttgatt ttttggctgt acacccgatg agggtaattg agaggatgaa tgcgagggtg
      241 cttattcgct taattgctgt ttgctgtttg cacggatttt atttccatcg tgatttctcc
      301 aacgagttta ttttaaaaga ggtcattttt tcctcttctg aattgctgca tcgtttatga
      361 cagacctctt ttttgtgtca agcaaatttg tacgaacttc tataacgagg gataacggac
      421 agaatctgaa gaaaagtctt ctgaacagat tgtaggaact gctatcgagg aaaaaaggga
      481 gctttggctg tcaccggtat ccgttttttt cagtcctcga ggtttcaaaa acctcgggac
      541 tttgcagaca actgcaatgt aaaattttcc agatctatta tttgtccttc agttttttca
      601 taagttcctc tatcagtttg tctttttctt caattgtctt ttccttttct tcaattgtct
      661 tcctgctttc ttcaattgtc ttcctgcttt cttcaattgt cttttccttt tcttcaattg
      721 tcttttcctg gttttcgatc ttcctttcca gatcctgaag ttccatcaga atttcctctt
      781 ccaaatccat ctgacgtttc agtgtttcat ctgaaactgc atagttcaga cgttgtacaa
      841 tcttctgctg cagttcgtca tcaagaggat aatcgtaata aacgatatga tccttggaag
      901 gataggcacg gtacccgtct ctgcttgaac tgtgactgaa gacactgagt actttttcca
      961 gccggtttct catttccgtc ttcagttcaa acagctgaat tacaaacgaa tcatgcgtca
     1021 gactgtcaat aa
//
LOCUS       DAOQGJ010001962         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_129069_length_1032_cov_3.112590, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001962 DAOQGJ010000000
VERSION     DAOQGJ010001962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129069_length_1032_cov_3.112590"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1032)
                     /locus_tag="PK683_22890"
     CDS             complement(<1..>1032)
                     /locus_tag="PK683_22890"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNH11365.1"
                     /translation="DVEITAFYPRNLEASGKEESFALSAIRPDVEIFLQKLTAINGRF
                     KSKFINADVETELLGEYGQISNGSILVRSLKTGSNIGGNPYLEEKVMVQNKKDLEGLE
                     RKLVQAIHNVSLPQKKFYFTKLNGERYGTNTATVPDENTEKFISALGLFNFQVKGLGL
                     EEGWPGKIPDDADIVGIIGPTTAFSDEAQKTLLEYVKNKNGRVFITIDPKEKEDFKWL
                     LDKSKLEFKKESLRQAQGRVEIIATNFPEHPISSLFTKKETGVVFPWSGFFEKKNTGG
                     SEFFETNILETGSAIFIDKNNNNQLDKEEKQGNFILGSILTTVDPNTKDAKEKPYGKV
                     IVFSGTSWLT"
BASE COUNT          240 a          256 c          199 g          337 t
ORIGIN      
        1 gtcagccagg atgttccgga gaaaacgatc actttcccgt aaggtttttc cttggcatcc
       61 ttagtattcg gatccacagt cgtcaggatt gatcccaaaa tgaaattccc ctgcttttct
      121 tccttgtcca gctgattatt attgttttta tcaataaata ttgcactgcc tgtttccaga
      181 atgttcgttt caaaaaactc ggaaccgccc gtgtttttct tttcaaagaa tcccgaccag
      241 ggaaacacta cacctgtttc tttctttgtg aagagactgg atatcgggtg ttcagggaag
      301 ttggtcgcaa tgatttccac acgcccttga gcctgtctca ggctttcctt tttaaattca
      361 agcttgcttt tgtcgagaag ccatttgaag tcttccttct ctttgggatc tattgtaatg
      421 aaaactctcc catttttatt cttgacatat tccagaagag tcttctgtgc ctcatccgaa
      481 aaagcagttg taggtccgat gattcccaca atatcagcat catccgggat ttttccaggc
      541 cagccctctt ccagtccaag tccctttacc tgaaaattga acagtcccag agcagagata
      601 aacttctcgg tattttcatc cggcacagtc gcagtattcg ttccgtacct ttccccgttc
      661 agtttggtga aatagaactt tttctgagga agactcacat tgtgaattgc ctgcaccagt
      721 tttctttcca ggccttccag atccttcttg ttctgcacca tcaccttttc ttcaaggtaa
      781 ggatttcccc cgatattgct tccggtcttc agacttctga ccagaattga tccgttggag
      841 atctgtccgt attcgcccaa aagttcagtt tccacatccg cattgatgaa cttggacttg
      901 aatcttccgt tgattgcagt cagtttctgc agaaagattt ccacatcagg acggatggct
      961 gagagtgcaa aactttcctc tttaccggag gcttcgaggt ttctaggata aaaagcagtg
     1021 atttccacgt ct
//
LOCUS       DAOQGJ010001963         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_129164_length_1032_cov_2.087001, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001963 DAOQGJ010000000
VERSION     DAOQGJ010001963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129164_length_1032_cov_2.087001"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /gene="eutB"
                     /locus_tag="PK683_22895"
     CDS             complement(<1..350)
                     /gene="eutB"
                     /locus_tag="PK683_22895"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNH11366.1"
                     /translation="MAKASPKRSGDELAGVSGESEEERAAAQTVLADVPLKKFLTEEL
                     IPYEKDEVTRLIMDSHDKKSFSPVSSLTVGEFREWLLNEHTAGDLIRKASKGITPEMA
                     AAVSKIMRISDLISV"
     gene            complement(449..>1032)
                     /locus_tag="PK683_22900"
     CDS             complement(449..>1032)
                     /locus_tag="PK683_22900"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH11367.1"
                     /translation="EINESAKGISDIMSAINDIADQTQLLSLNASIEAARVGELGKGF
                     AVVAQEIRKLAESSTLSAENARKLISKNSATISKGTDSMNGISSSLQNLFENIAQNTG
                     RINVMAEEFSLQTKSLSESNSRIQQLDALSQEIRDYARSQAEANSRIKDSIEIIRSQS
                     ADFLEIAKDLQERSARFKSESKELEEIIKKFKY"
BASE COUNT          252 a          237 c          204 g          339 t
ORIGIN      
        1 acagaaatca aatcggatat tctcataatc tttgaaacag cagctgccat ttccggagtg
       61 attcctttgg atgctttccg gatcaaatct cccgctgtat gctcattcag aagccactct
      121 ctgaattctc ccactgtaag tgaagatacc ggagaaaatg attttttatc atgggagtcc
      181 atgatgagtc ttgtcacctc atccttttca taaggaatca gctcttctgt gagaaatttt
      241 ttcagaggta catcagcaag gactgtctga gcagccgctc tctcttcttc agactctccg
      301 gatacacccg cgagttcatc cccggatctt tttggagagg cttttgccat caaagtcttc
      361 aggctgctga actgataatg ctttcctcgt atcttgatct tgtacatgca ggaaatttta
      421 tgaaatgaaa ataaacgtca agtgaaaatc agtatttgaa ttttttgatg atttcttcaa
      481 gttcctttga ttcggacttg aatcttgccg atctttcctg aagatccttt gcgatttcga
      541 gaaagtctgc agactggctt cggatgattt ctatggagtc ttttatcctg gaattcgctt
      601 ctgcctgact tctggcataa tccctgattt cctgtgaaag cgcatcaagc tgctgaatcc
      661 tggaattgga ttcggaaaga cttttcgtct gcagagaaaa ctcttccgcc atgacattga
      721 ttcttcctgt attctgtgca atgttttcaa aaagattctg aagactggaa ctgattccgt
      781 tcatcgagtc agttcccttg ctgattgtag ccgaattttt ggaaatcagc tttcttgcat
      841 tctctgcaga aagagtcgaa ctttcggcaa gtttacggat ttcctgcgct acaacagcaa
      901 acccttttcc cagttcaccc accctggctg cttcaattga tgcatttaga gaaagaagct
      961 gagtctggtc tgcaatgtca ttgatcgcgc tcataatatc tgatatgccc ttcgcgctct
     1021 cattgatctc tt
//
LOCUS       DAOQGJ010001964         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_129267_length_1031_cov_4.052254, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001964 DAOQGJ010000000
VERSION     DAOQGJ010001964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129267_length_1031_cov_4.052254"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..510
                     /locus_tag="PK683_22905"
     CDS             124..510
                     /locus_tag="PK683_22905"
                     /inference="COORDINATES: protein motif:HMM:NF024736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNH11368.1"
                     /translation="MKKSVYLKDEHWEIIKPYIKEPKPREDGRGRERRDPRAVLEGIL
                     WVLKTGARWRDLPRPQYPPYQTCHRRFQEWIKNGSIYDLLRGLARILKDEGIIKTGES
                     YIDGSFASAKKGVSKSGKPRKARVQR"
     gene            510..911
                     /locus_tag="PK683_22910"
     CDS             510..911
                     /locus_tag="PK683_22910"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNH11369.1"
                     /translation="MAVADSGGLPIGMRLDSASPHEAKLAVDTVSSIFVEGKIENLIG
                     DKAYDSDPLDKKLKEGYGIDLTAPHKSNRKKTKTQDGRKLRRYLRRWKIERLFAWLQN
                     FRRLVVRYEVKEQNFLAFIHLSCCCIYLRYF"
BASE COUNT          343 a          189 c          243 g          256 t
ORIGIN      
        1 atcatacata taggatatcc cattattgca tatcaataca caaatttcta gaactggtct
       61 caaaaaccaa cataaaggaa gtaaattact tgtacaaaaa tcaggataat ttgcagtatc
      121 cttatgaaga agtcggtata tctgaaagat gaacattggg agataataaa gccgtatata
      181 aaagagccta agcctcgtga agacggtcgt ggacgcgaaa gaagagatcc gagagcagta
      241 ttggaaggga tactgtgggt tctgaaaacc ggtgcaaggt ggagagatct gcctcgtcct
      301 cagtaccctc cgtatcagac ctgtcacaga aggtttcagg aatggataaa aaacggttca
      361 atttatgatc tgctgagagg gcttgccagg atattgaaag atgaagggat cattaaaacc
      421 ggagaatcct atatagacgg ctcatttgcg agtgcaaaaa aaggggtctc aaagtcggga
      481 aaaccaagaa aggcaagggt acaaagataa tggctgtcgc agacagcggc ggccttccaa
      541 ttggtatgag gctggacagt gcgtctcctc atgaagcaaa actggcagtc gatactgtca
      601 gcagcatttt tgttgaaggc aaaattgaaa accttattgg cgacaaggct tatgactcag
      661 atcctcttga taaaaagctg aaagagggat atggaataga tctgactgct ccgcacaaat
      721 ccaacagaaa aaaaacaaaa acgcaggatg gaagaaaact gagaagatat cttcgacgct
      781 ggaaaatcga aagacttttt gcctggctcc agaatttcag aaggcttgtt gtgcgctatg
      841 aagtcaagga acagaacttt cttgctttta tacatctttc atgctgttgc atttacctga
      901 gatatttttg agaccagttc tagaaatctg ggcactattc cttgatgtcc atcagaaggc
      961 ccctgatttt ttctatctgt ttcagaagag aaaatgcaga gtttgcagga ttcagcatag
     1021 tcattgccag g
//
LOCUS       DAOQGJ010001965         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_129278_length_1031_cov_3.505123, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001965 DAOQGJ010000000
VERSION     DAOQGJ010001965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129278_length_1031_cov_3.505123"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PK683_22915"
     CDS             <1..703
                     /locus_tag="PK683_22915"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNH11370.1"
                     /translation="RKGGTGSEKTAGLKKRNVPTVLNAGYYSSYYWDGKFKELQKQIL
                     SHIQEEMGIGPASLESRLSPEYLDLIAKALPSNTKLNLEYIALSLSAYLHTLKTKDRF
                     DDFVKGNFKALSFEEISGMETFIAVGCSDCHSTYLLGGGFRKMGEKNPYKNISDLGRF
                     TVTNEEQDKMIFRVPSLRNIALTGSYFHDGSTADLEDAISEMAFLQLNKRLSDRDIQK
                     IAVFLKALTDKDRER"
     gene            711..>1031
                     /locus_tag="PK683_22920"
     CDS             711..>1031
                     /locus_tag="PK683_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11371.1"
                     /translation="MARNKFRFTLNPFEIMDDIVRELNPFQVLIGAVEDSKDNYRVDI
                     DMRYVNELVQLEVLEESENLLDLDIFALDGSLYVKGEYHIGDYPLAGLLNLHEVIFNA
                     EFVPI"
BASE COUNT          317 a          193 c          239 g          282 t
ORIGIN      
        1 caggaaaggg ggaacaggga gcgagaagac agccggactg aaaaaaagaa atgtgcctac
       61 tgttctgaat gcaggatact attccagtta ttactgggac ggaaaattta aagaacttca
      121 gaagcagatt ctttctcata ttcaggaaga aatgggaatt ggaccggctt ctcttgaatc
      181 cagactctct cccgaatatc tggatctgat tgcgaaagca ctaccctcaa atactaaact
      241 gaatctggaa tatattgctc tttccctgtc tgcgtatctg catacactga agacaaaaga
      301 ccggtttgat gattttgtaa agggaaattt caaggcactg tcctttgaag agatttcggg
      361 aatggagacg ttcattgcag tcggatgttc agactgtcac agcacttatc ttcttggggg
      421 cggattccga aaaatgggag agaagaatcc ctataaaaat atctctgatc tgggaagatt
      481 tactgtaaca aacgaagaac aggacaaaat gattttcaga gtcccctctc tcagaaacat
      541 tgctcttaca ggaagttatt ttcatgatgg gagcactgcc gatctggaag atgcgatttc
      601 tgagatggca tttctgcagc tgaacaaaag actttcagac agagatattc agaaaattgc
      661 tgtttttctg aaagcactta ccgacaagga cagagaaaga taatcagagt atggccagaa
      721 acaagttcag attcacttta aatccgtttg aaatcatgga tgacattgta cgggagctga
      781 atcctttcca ggttctcatt ggagcagtgg aggattccaa ggacaattat cgcgtggata
      841 ttgatatgcg gtatgtaaat gaacttgtac agcttgaggt tctggaagaa tccgaaaatc
      901 ttctggatct cgatatcttt gcactcgacg gaagtcttta tgtaaagggc gaatatcata
      961 tcggcgatta tcctcttgca ggacttctga atctgcacga agtgatcttc aatgcggaat
     1021 ttgtgcctat c
//
LOCUS       DAOQGJ010001966         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_129348_length_1031_cov_2.566598, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001966 DAOQGJ010000000
VERSION     DAOQGJ010001966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129348_length_1031_cov_2.566598"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PK683_22925"
     CDS             <1..290
                     /locus_tag="PK683_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNH11372.1"
                     /translation="KDQQRIGGKDLGDIDSALENILRYRTEVGAKQNRVENHAERVAF
                     DKTFMSELLSNNEGVDFPEAIMNLKWLETVHSYSLNIGSRIIRPTLLDFLK"
     gene            287..736
                     /gene="fliW"
                     /locus_tag="PK683_22930"
     CDS             287..736
                     /gene="fliW"
                     /locus_tag="PK683_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNH11373.1"
                     /translation="MMREIDSKPFGKIQIDERQIVDFPEGLFGFDQNRSFAVIEENPD
                     TVFKWLQSLEDPELAFVLIQPHIFLTDYKPLIPPTELKTIGLNSVENAVLFLIVTIPA
                     SDPKKMTANLQGPILIHPDTRIAKQFISRDENHPLRRLILDTEESEK"
     gene            822..>1031
                     /gene="csrA"
                     /locus_tag="PK683_22935"
     CDS             822..>1031
                     /gene="csrA"
                     /locus_tag="PK683_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNH11374.1"
                     /translation="MLVLARRLNQSILIGEEVEIVVVEIKGDQVKLGIKAPKNVSVHR
                     AEVLKEIQEENKKAKADILPTNLSEL"
BASE COUNT          321 a          197 c          231 g          282 t
ORIGIN      
        1 ccaaggatca gcagagaatc ggaggaaagg atctgggaga tattgattca gctcttgaaa
       61 atattctgcg ttacagaact gaggtcggtg caaaacagaa ccgtgtggaa aaccatgcgg
      121 aaagagttgc atttgacaag acattcatgt ctgaacttct ttccaataat gagggagtgg
      181 atttcccgga agcgattatg aatctgaaat ggctggaaac cgttcattct tattcattga
      241 atatcggttc caggattatc cgtccgactc ttctggactt tctgaaataa tgagggaaat
      301 tgacagtaaa cctttcggga aaattcagat tgatgaaaga cagattgtag atttcccgga
      361 gggtctgttc ggttttgatc agaacagatc ctttgctgtc atcgaagaga atcctgatac
      421 tgttttcaaa tggcttcagt cactcgaaga tcctgaattg gcatttgtac tgatccagcc
      481 ccatattttt ttaactgatt ataaaccgct cataccgccc accgaactga aaacaatcgg
      541 tctgaattca gtggaaaatg cagttctgtt tctgattgtc acgattcctg cttcagatcc
      601 gaaaaaaatg actgccaacc tgcaaggtcc gatactcatt catcctgata caagaattgc
      661 caagcagttt atttcgaggg acgaaaatca tcctctccgc agactgattc tggacacaga
      721 ggaatcggag aaatgaacag cgatttgtct tataatgaaa atttaatgaa taatttggac
      781 agaatgcgga aagaatctgt tttctttttc aggacaatca gatgttagtt ctcgcaagaa
      841 gactcaatca gtccattctg atcggtgagg aagttgagat agttgtagtc gaaatcaagg
      901 gcgatcaggt caaacttggc atcaaagctc ctaaaaatgt atcagtgcac agggctgagg
      961 ttctgaagga aattcaggaa gagaataaaa aggccaaagc agatattctt ccgacaaatc
     1021 tttcggagct c
//
LOCUS       DAOQGJ010001967         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_129530_length_1030_cov_4.296410, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001967 DAOQGJ010000000
VERSION     DAOQGJ010001967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129530_length_1030_cov_4.296410"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1030
                     /locus_tag="PK683_22940"
     CDS             <1..>1030
                     /locus_tag="PK683_22940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016757024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNH11375.1"
                     /translation="EMRNISVLLIPFIIVIIPMGLIAKQPDFGTAMSFLPILLAMLYL
                     GGADMMHISSLIILGGVSLVIPLYIEYTKISLVEPLVEYLAKIEKAELANIVNQMKGK
                     VWMLMRGKEVKIGEKAVNLSPKSMKVLNEASEQVIEDNTGFLFKTFSNDDFMIGLALV
                     FLIVSFVLIMLRYARGSMQFRRYYIPLGMVGLSILAAVTLNRVAPFSENQVGRFTAFI
                     NPEKFRQGAGYQLLASKPAIGSGRILGKGLMNAEMTEGKTPHVPESGTDFIFASWAEQ
                     TGFFGSVILLFFILAIPLRGLQISFESKDRFGSLLAAGVVALIFFHMLINLGIVLGLM
                     PVTGLPLSF"
BASE COUNT          248 a          213 c          256 g          313 t
ORIGIN      
        1 ggagatgaga aacatttcgg tgcttctgat ccctttcatc attgtcatta ttcccatggg
       61 tctgattgca aagcagcctg acttcggaac tgcaatgtca tttcttccaa ttcttctcgc
      121 catgctgtat ctgggcggtg cagatatgat gcatatcagt tcacttatta ttctgggtgg
      181 ggtttctctc gtaattccgc tgtatataga gtatacgaaa atttcccttg tagagccttt
      241 ggtggagtat cttgcaaaaa ttgaaaaggc ggaactggcc aacattgtca atcagatgaa
      301 aggtaaagtc tggatgctga tgcggggcaa ggaagtcaag atcggggaaa aggcggtgaa
      361 tctttctcct aaaagcatga aggtgctcaa tgaggcttcc gagcaggtga ttgaagacaa
      421 tactggattt ctgttcaaaa cattcagtaa tgatgatttc atgattggac ttgccctagt
      481 atttctgatc gtcagttttg tgctgattat gctcagatat gccagaggtt ccatgcagtt
      541 caggagatat tatattccgc tgggaatggt gggtctgagt attctcgcag ccgtaacctt
      601 aaacagagtg gctccgtttt ctgaaaacca ggtaggacgt tttactgctt tcatcaatcc
      661 ggagaaattc cggcagggtg caggatatca gcttctggct tccaagccgg caatcggatc
      721 cggcagaatt ctcggtaagg gactgatgaa cgctgaaatg acagagggaa aaacacctca
      781 tgtgcctgag agcggaaccg actttatatt tgcatcctgg gcagaacaga caggcttttt
      841 cggctcagtc atcctgcttt tctttatttt ggcgatacct ctgcgaggtc tgcagatcag
      901 ttttgagagc aaggacaggt ttggctccct tcttgccgcc ggtgtcgttg ctttaatatt
      961 ctttcatatg ctgatcaatt taggtatcgt acttggactg atgcctgtca caggtcttcc
     1021 cctttcgttt
//
LOCUS       DAOQGJ010001968         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_129564_length_1030_cov_3.082051, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001968 DAOQGJ010000000
VERSION     DAOQGJ010001968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129564_length_1030_cov_3.082051"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..462
                     /locus_tag="PK683_22945"
     CDS             76..462
                     /locus_tag="PK683_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11376.1"
                     /translation="MKEEEIPPSTIEKVMEEIFKKGYRIEFEYGEVGNANFFDVKFKS
                     GTIFIKLNIKHPAYPHLIEVLDEEVEDQEAETLKERLVKARDGLKLLLIAWARFEDEQ
                     DGPARTRIQDTRHDWGRIARDFLSKE"
     gene            492..>1030
                     /locus_tag="PK683_22950"
     CDS             492..>1030
                     /locus_tag="PK683_22950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNH11377.1"
                     /translation="MLDRIKIDFEDVVRQKIISHPNLKKMKKNIDDFLSIIPYDFHLK
                     KYQKTALEVNRASHPKLFSYIDDVAGQFQIKANIRVFKAREMTGENALISADKDNVLI
                     VFCDNILNLINSELELKSIIGHEFGHYLFKHHEKKIEKYLQGIAGMFPEEAINNEEKK
                     YARSAENTELIDMAFIISQ"
BASE COUNT          393 a          144 c          226 g          267 t
ORIGIN      
        1 ggacatacgg gaacgacgga taagcagata aagactgaga cagaggatga gagaaagaaa
       61 agaattcaag aagaaatgaa ggaagaggaa attcctccat ctactattga aaaagttatg
      121 gaggaaattt tcaagaaagg ttatcggatt gaatttgaat acggggaagt aggaaatgcc
      181 aacttttttg atgttaagtt taaatctgga acaatattta taaaattaaa tataaaacat
      241 cctgcatatc cgcatcttat agaggtattg gacgaagaag tggaagatca ggaggctgaa
      301 acattgaaag aacgtctggt aaaggcgagg gacggcttaa aactgctgct cattgcatgg
      361 gcaagatttg aggatgagca ggatggtcca gccagaacaa ggattcagga tacaagacac
      421 gactggggtc gaatcgccag agatttttta tcaaaggaat agctttaaca acacagcaca
      481 aggaggatac tatgctggat agaattaaaa ttgactttga ggatgtggtc agacagaaaa
      541 taatttctca tcccaatctg aagaaaatga aaaaaaatat tgatgatttt ctttccataa
      601 tcccctacga ttttcacttg aaaaaatatc agaagactgc cctggaagta aatcgagctt
      661 ctcacccaaa attattctct tatatagacg atgttgcagg ccagtttcag attaaggcaa
      721 acatccgagt ttttaaagca agggaaatga caggggaaaa tgctttaatt tctgcagata
      781 aagacaatgt attgattgtt ttttgcgata atatattaaa tctgataaat tctgagttgg
      841 aacttaaaag tatcatagga catgagtttg gacattatct ctttaagcat catgaaaaaa
      901 aaatagaaaa atatttgcag ggtatcgctg ggatgtttcc agaggaagcc ataaacaatg
      961 aagaaaaaaa atatgcaaga agtgctgaaa atacagaact gattgatatg gcatttatta
     1021 tcagccagat
//
LOCUS       DAOQGJ010001969         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_129818_length_1029_cov_3.042094, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001969 DAOQGJ010000000
VERSION     DAOQGJ010001969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129818_length_1029_cov_3.042094"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PK683_22955"
     CDS             <1..574
                     /locus_tag="PK683_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNH11378.1"
                     /translation="SEEQPSHLKQYYEDTVQISKKLTEWALTKCKNPSRQDKFVICTG
                     GGPGIMEAGNKGARLAGGRSIALNIKLPHEQAANPYVDPNLTFTFHYFFMRKLWFMQL
                     AEAVVVMPGGFGTIDELFEVLTLIQTSKAAKIPIILYGKDFWNDLINFEKLADYKLIE
                     RSDLNLIYRTDSVDEAVEFIVTYLESDMFQ"
     gene            714..>1029
                     /locus_tag="PK683_22960"
     CDS             714..>1029
                     /locus_tag="PK683_22960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11379.1"
                     /translation="MADDSTKPSRFSVKKLSPSGMPYNDKGRFRSPFANPAYGTPCNI
                     KVGFMLKSVSGFSTKEGKFEADFYVSYTSDKPMPGEIKPHFTNGMVQDDDHIERIADE
                     PTF"
BASE COUNT          301 a          211 c          234 g          283 t
ORIGIN      
        1 gtcggaagag cagccctcgc atctgaaaca gtactatgaa gacactgttc agatatccaa
       61 aaaactgaca gagtgggcac tcacaaaatg caagaatccg tccagacagg ataagttcgt
      121 catctgcaca gggggcggtc cagggataat ggaggcaggc aacaagggtg caagacttgc
      181 aggggggaga agcattgctc tcaacataaa acttcctcat gaacaggcgg caaatccgta
      241 tgtggatccc aatctcacat ttacttttca ttattttttc atgcgtaagc tctggtttat
      301 gcagcttgct gaagcagttg ttgtgatgcc gggaggattc ggaacgattg atgagctttt
      361 cgaagtgctg acactgattc agacctcaaa agcggccaag atcccgatca ttctttatgg
      421 aaaggatttc tggaatgatc tgatcaattt tgaaaaactg gcagactata agctgattga
      481 gagatccgat ctgaatctca tatacaggac agacagtgtg gacgaggcag tggagtttat
      541 agtaacttat ttggaatcag atatgtttca atgatttaaa tgaattgacc tttctctact
      601 tttctgcaag tttggtcttc ggtcaaggta aaatgagatt tctttctaaa attcccaact
      661 gcagatattt atgttttccc cttttctgcc tgctcctgtt tccgtcagcg catatggcag
      721 acgattccac caaaccgtca agattttcag taaaaaaatt atctccctcg ggaatgccct
      781 acaatgacaa aggaagattc aggtctccgt ttgcaaatcc tgcttacggg actccgtgca
      841 acatcaaagt agggtttatg ctgaaaagtg tttccggctt ttcaacgaag gaaggaaagt
      901 ttgaagccga tttttatgtt tcctatacaa gtgacaaacc tatgcccggg gaaattaagc
      961 cccatttcac aaatggtatg gtgcaggatg acgatcatat agaacggatt gcagatgaac
     1021 cgacattca
//
LOCUS       DAOQGJ010001970         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_130083_length_1028_cov_2.904419, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001970 DAOQGJ010000000
VERSION     DAOQGJ010001970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130083_length_1028_cov_2.904419"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PK683_22965"
     CDS             complement(<1..452)
                     /locus_tag="PK683_22965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNH11380.1"
                     /translation="MNKELKTAAECLSGLSDGMTLMAGGFGLCGIPEHFILAIRELGI
                     RNLTVISNNCGVDDFGLGILLQTRQIRKMISSYVGENKTFEKQFLSGELEVELVPQGT
                     FAERIRAGGAGIPAFYTAAGVGTAVAEGKETKVFDGREYIMEKALRAD"
     gene            542..>1028
                     /locus_tag="PK683_22970"
     CDS             542..>1028
                     /locus_tag="PK683_22970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH11381.1"
                     /translation="MPLDTSQTNQKISIPSLQILFEEGAASNSLNILHEGAYSLEKKF
                     GDTSLPLLQVSGKNLTPGVISLFGNGRYPCSIKTTHPSVISTYTVTTATIHKSIQSKM
                     SLGVMIARTLLREILELTKKMNAISSLSSGVEQIRDNMALAYYSMEPSMFKDIEKERY
                     IY"
BASE COUNT          286 a          262 c          202 g          278 t
ORIGIN      
        1 aaatccgccc gaagagcttt ttccatgata tactcccttc cgtcgaaaac cttcgtctcc
       61 tttccttccg caactgcagt gccgacgcct gcagcagtgt agaatgcagg aattccggct
      121 cctcccgcac gtatccgttc tgcaaatgtt ccctgcggga caagttccac ttctagttcg
      181 cccgacagaa actgtttttc gaaagtcttg ttctcaccga cgtagctcga gatcattttg
      241 cggatctgtc ttgtctgaag aaggattccc agtccaaaat cgtccacccc gcagttgttg
      301 gagattacag tcaggtttcg tattccgagt tctctgattg cgaggataaa atgctcgggg
      361 attccgcaga gaccgaatcc tcctgccatc agtgtcattc cgtcactcag tccggagagg
      421 cattctgcgg ctgtcttcaa ttctttattc atattaaagt ccgaaaagat aaaattcttg
      481 taaaaacagt ctgccctgac aaagtgtttc ttgccaaacc aaaaaataag gttttttcaa
      541 tatgccactg gatacttcac agacaaacca aaagatttcc attccgtctc ttcaaatcct
      601 ttttgaagag ggagcagcaa gcaattcact gaatattctt catgaaggag cctacagcct
      661 tgagaaaaag ttcggagata caagcctgcc tcttcttcag gtcagcggta aaaatcttac
      721 tccgggagtc atcagccttt ttggaaacgg aagatatccc tgctcaatca agacgactca
      781 tccttcagtc atttcgacat atactgtcac aactgcaaca attcataaat ccatccagtc
      841 caagatgtcc cttggagtaa tgatagcgag aacacttctc cgtgaaatcc tggaactgac
      901 aaagaaaatg aacgcaatca gctctctgtc ctcaggcgtg gaacagatcc gggacaatat
      961 ggctcttgca tactattcca tggaaccttc gatgttcaag gacattgaaa aggaaagata
     1021 catatatt
//
LOCUS       DAOQGJ010001971         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_130324_length_1027_cov_3.327160, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001971 DAOQGJ010000000
VERSION     DAOQGJ010001971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130324_length_1027_cov_3.327160"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PK683_22975"
     CDS             <1..891
                     /locus_tag="PK683_22975"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH11382.1"
                     /translation="VSLIFGLVEADTGLVYYINAEHPWMILYRDGIADFAERDSYFRK
                     LGTSGVKNEIFVSTFRMMPGDLLIIGSDGKDDVVLSEAQENSDRVINEDETLILKRVE
                     EAKGDLNGIFKVITGKYKLMDDLSLMSIYYKGETEEDIEEKSKITKYIKRAAKYFQDG
                     NYETSLKIMEEARTVHPSNFTLVNYTLQLYMKMKMFDKGAELCRQYLKSNEANTAFMI
                     KASYCLKMNQDLEAAIEIAERVKLREPGNVRNLIHLSDMYAYTKNWKRAKKLLDKVKL
                     IDPGNQKAEKVRHVLEDAYS"
BASE COUNT          342 a          201 c          247 g          237 t
ORIGIN      
        1 gtatcactga ttttcggact tgtggaagcg gatacagggc ttgtctatta catcaacgct
       61 gaacaccctt ggatgattct ttacagagac gggattgccg actttgccga aagagacagt
      121 tatttcagaa agctgggaac gtccggagtg aaaaatgaaa tattcgtttc cactttcaga
      181 atgatgccgg gcgatcttct gatcatcggt tctgacggaa aagatgatgt ggttctctct
      241 gaggcacagg aaaacagtga cagggtcatc aacgaagacg agactctgat tctgaagcgc
      301 gtagaggaag caaaaggcga cctgaacgga atattcaagg tcattacagg aaaatacaag
      361 ctgatggatg atctttctct gatgtcgatt tactataagg gagagacaga agaagatatt
      421 gaagaaaaaa gcaaaattac aaagtatata aaaagagctg caaaatattt ccaagacgga
      481 aactatgaaa cctctctgaa aataatggag gaggccagaa ctgtccatcc atccaatttc
      541 acactcgtga attatactct tcagctttat atgaagatga agatgtttga caagggagcg
      601 gaactctgca gacagtatct gaaatcaaac gaagcaaata ctgcatttat gatcaaagcc
      661 tcctactgtc ttaaaatgaa ccaggatctg gaagcagcaa tcgagattgc tgaacgtgtc
      721 aaactcaggg agccgggcaa tgtgagaaac cttatccatc tttcagatat gtatgcatac
      781 accaaaaact ggaaacgtgc taagaaactt ctggacaaag tgaaactgat cgatcctgga
      841 aatcagaagg cagaaaaggt caggcatgtt ctggaagatg cctacagctg aaggctggcc
      901 cacagctgaa ggctggctca cagctgaaca ggtgtcactc tgcagccggt tttggaatgc
      961 ttgtcaaaat gaaaacagct gcaatgagtg cagctgcggc tgtcagaata aaactgaccg
     1021 atgcacc
//
LOCUS       DAOQGJ010001972         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_130333_length_1027_cov_3.175926, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001972 DAOQGJ010000000
VERSION     DAOQGJ010001972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130333_length_1027_cov_3.175926"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /gene="rpsE"
                     /locus_tag="PK683_22980"
     CDS             <1..160
                     /gene="rpsE"
                     /locus_tag="PK683_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560639.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNH11383.1"
                     /translation="AGVQDVLSKSYGSGNPMNIVKATYEALKQLETPLMVAKKRGVTL
                     KKLFGQEF"
     gene            177..356
                     /gene="rpmD"
                     /locus_tag="PK683_22985"
     CDS             177..356
                     /gene="rpmD"
                     /locus_tag="PK683_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNH11384.1"
                     /translation="MEKVKITQVRSCIGVIPNHVKVVNALGLGRIGKTRVHELTPPIK
                     GMLRKVGYLVKQEKA"
     gene            358..894
                     /gene="rplO"
                     /locus_tag="PK683_22990"
     CDS             358..894
                     /gene="rplO"
                     /locus_tag="PK683_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNH11385.1"
                     /translation="MANTDFAKRSEIYKGKRTKKASKKANTSNSVPVPKNSRKDKKRV
                     GQGPGSGMGKTSTRGQKGQRARASSMRPGFEGGQMRLSLRMPKRGFTNIFKTVFQPVN
                     LSVLAKLNLSGEVTPALLEEKGLIKSAEGKVKFLGSGELKSALKLVGDAISYSAKEKL
                     EKAGGSFSARTAEKKTKE"
BASE COUNT          363 a          183 c          230 g          251 t
ORIGIN      
        1 agcaggggtt caggacgttc tttccaaatc atacggatca ggaaatccaa tgaacatcgt
       61 gaaggctact tacgaagcac tgaaacagct ggaaactccg cttatggttg ctaaaaaacg
      121 cggtgttaca ctgaagaaac ttttcggtca ggaattctga gagaggaata ttcaatatgg
      181 aaaaagttaa aataactcaa gttagaagct gtataggtgt cattcctaac catgtaaagg
      241 tggtaaatgc tcttggactc ggaagaatcg gaaagacaag agtacatgaa ttgactcctc
      301 cgataaaagg tatgctcaga aaagtgggat accttgtaaa acaggagaaa gcataaaatg
      361 gcgaatactg attttgcaaa aagatctgag atttacaaag gaaaaagaac aaagaaggct
      421 tctaaaaaag caaatacttc caattccgtt cctgtcccta agaattccag aaaagataaa
      481 aaaagagtag gacaaggacc tggttccggg atgggaaaaa catccactag aggacagaaa
      541 ggtcagagag caagagcttc cagtatgaga cctggatttg agggcggaca gatgagactt
      601 tctcttcgta tgcctaaaag aggctttacg aatattttca agactgtttt tcagcctgta
      661 aatctttccg ttctcgcaaa actgaacctg tccggcgaag tgactccagc actgcttgaa
      721 gaaaagggac tgattaaatc tgcagaagga aaagtaaaat tcctcggatc aggtgagctg
      781 aagtctgctc tgaaacttgt cggagatgca atatcttatt ctgctaaaga aaagctcgaa
      841 aaagccggtg gaagtttttc tgcaagaact gcagagaaga agactaaaga gtaagaaaat
      901 ttatgctttc agccattacg acaatattta aaatcacaga acttagaaac agaattgttt
      961 ttacggtgac aatgctcctt ctgttccgta tgggaactca tgttacaatt cctggtatca
     1021 acagtct
//
LOCUS       DAOQGJ010001973         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_130405_length_1027_cov_2.301440, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001973 DAOQGJ010000000
VERSION     DAOQGJ010001973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130405_length_1027_cov_2.301440"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..598)
                     /locus_tag="PK683_22995"
     CDS             complement(119..598)
                     /locus_tag="PK683_22995"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNH11386.1"
                     /translation="MRRLFILPALLAVFCSSRKKTHQLPQILFPSFSSKAFFSYPGKF
                     EDPIRKRHVLDHILSLIKGSEKSLRFYVYSFNHPEIVSELKAASGRGVKIEIQGDRNT
                     DYSLLKKNGIPYRVWSENGIHHLKIIISDEKKTFSRHWKLLLSRNHERLERIHSAAA"
     gene            complement(595..>1027)
                     /locus_tag="PK683_23000"
     CDS             complement(595..>1027)
                     /locus_tag="PK683_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11387.1"
                     /translation="GNIIESGYVGMGNGPERSVLLFQTLGFMPSAWVTEEGMEKRFSI
                     VKGRRNSFYSGISAEYARNGYFFRAEADCTVPYANSSSSNGKIGISRNSFEPWFIAEG
                     ALSAEFRENDFFMKMRYSHLWTQKNISVTGDMISFSGGFLF"
BASE COUNT          303 a          229 c          208 g          287 t
ORIGIN      
        1 gtcctgaatc agcactccct tttcaggaga aaacataaaa atgaatcctc caatgctaat
       61 cccaggctca gggctcctgc cttccaggaa cgaaaggagt ccaatgtatt cattcttatc
      121 aagcggcagc tgaatgaatc cgttccagtc gttcgtgatt ccgggaaagg agaagtttcc
      181 agtgcctaga aaaagttttt ttttcatctg aaataatgat ttttaaatga tgaattccgt
      241 tctccgacca gaccctgtaa ggaatcccgt tctttttcag aaggctgtag tccgtattcc
      301 tgtcaccctg tatttcaatt tttactcctc tcccggaagc tgctttcagt tcagaaacaa
      361 tttccggatg attgaatgaa tacacgtaga agcgcaggct tttttccgat cctttgatga
      421 gcgaaagaat atgatcgagt acatgtcttt tgcgaatcgg gtcttcaaat ttgcccggat
      481 aggaaaaaaa tgccttactg ctgaaggaag ggaaaaggat ctgaggaagc tgatgagttt
      541 tttttcttga agaacagaaa acagcaagaa gagccggtaa aatgaacaga cgtctcaaaa
      601 cagaaatcct cctgaaaatg aaatcatgtc gccagtgacc gatatgttct tctgcgtcca
      661 gagatgagag tatctcattt tcatgaaaaa atcattttct ctgaattcag cagacagtgc
      721 cccttctgca atgaaccagg gttcaaaact gttcctggaa atgccgattt tcccgttcga
      781 tgatgaagaa ttcgcatatg gaacagtgca gtctgcttcg gcccggaaaa aatagccgtt
      841 ccttgcatat tcagcagaaa ttccggaata gaaggaattt cttcttcctt tcacaatgga
      901 aaatcttttt tccattcctt cctcagtcac ccaggcagaa ggcatgaatc caagagtctg
      961 aaaaagaaga acagaccgct caggtccgtt tcccattccg acatacccag attcaataat
     1021 gtttcct
//
LOCUS       DAOQGJ010001974         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_130598_length_1026_cov_2.947477, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001974 DAOQGJ010000000
VERSION     DAOQGJ010001974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130598_length_1026_cov_2.947477"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PK683_23005"
     CDS             <1..292
                     /locus_tag="PK683_23005"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNH11388.1"
                     /translation="GKLNGISLSRNGLDNADFLSNLKNLRFLNLNENYISDIQFISYM
                     KRLTYLDLSQNRIRKISSLQTLKELTHLNLSGNLIEDPETIGRLTNLTHIVF"
     gene            complement(727..>1026)
                     /locus_tag="PK683_23010"
     CDS             complement(727..>1026)
                     /locus_tag="PK683_23010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11389.1"
                     /translation="QLKEMTFINNLDSVLAYNAFNAPILFIFPLLECIQLQRICIWSG
                     VPLKDQCTFRRTDRKQITAFEWRVCRFFVRSDRKNLLGIRILLSKKLFRHSPARF"
BASE COUNT          322 a          203 c          206 g          295 t
ORIGIN      
        1 gggaaaattg aacgggattt ccctttcacg gaacggactg gacaatgctg attttctttc
       61 caatttgaaa aacctgagat tcctgaattt aaacgaaaat tatatatcgg atatacagtt
      121 tatatcttat atgaaaagac tgacttacct ggatctaagt cagaaccgaa tcagaaagat
      181 cagttcactc cagacgctga aagagctgac acatctgaat ctttccggaa acctcataga
      241 agatccggaa accataggca ggcttacaaa tctgacacat atcgtcttct gagcatctca
      301 acttacgctt acggcggctt gatggtccgc aaggtacgga tgctgctgcc ctcgcgaaaa
      361 ggacggataa acttccgttt ttaccgattc actggatttc tctaatgttt tctgaaaatt
      421 actgcgtcta tactgtatat cctatttgcg caaggtgaat gagtaaaaaa agatgagtta
      481 tatctgactg aatctctcag gaaaatcctt ttttcattct gcgggaaact gctgaaaaaa
      541 atataaagta attattttat atgatagaac agtctgcctt gacttgctga aaaaaagaga
      601 aatcaatccg ttttaataaa aaaaacattc tccatacgga tagaacagat cctgcaattt
      661 tagactgtgc agcagtcgca gattttaaga tctttcggaa tgaaatcagg gtcttcttcc
      721 ttgcgtttaa aaccttgcag gagaatgtct gaacagcttt ttcgaaagga gtatcctgat
      781 gccgaggaga tttttgcggt ctgaccgtac aaagaatctg catacgcgcc attcaaatgc
      841 agtgatttgc tttctatcag tgcgtcgaaa ggtacactga tccttcaggg gcactcccga
      901 ccatatacat attctttgca gttgaatgca ttccagaagc gggaaaataa acagaatcgg
      961 tgcattaaag gcattgtagg caagaaccga atccagatta ttgatgaatg tcatttcttt
     1021 cagctg
//
LOCUS       DAOQGJ010001975         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_130848_length_1025_cov_2.842268, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001975 DAOQGJ010000000
VERSION     DAOQGJ010001975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130848_length_1025_cov_2.842268"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..>1025)
                     /locus_tag="PK683_23015"
     CDS             complement(81..>1025)
                     /locus_tag="PK683_23015"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HNH11390.1"
                     /translation="ARTGQEQLCYGILDHDKGITDRYLELQKTVSRLNETADDFISED
                     FTAETAVLYDIENVRNLKYQPISEGLTEKVNDFLDIGFDKEFFTWYAGLNILNVPTHI
                     LPASETDLKKYRMIVLPLYIQTDPEFVKRLENFVADGGILVLGYRSGTKQKNGWMTDD
                     VPPGPFKDLAGIKIRKFESLGKNTLNISFGILSHKAGKIAEILEPDTAQVLARYRAKK
                     KFYHGRPAITVNRYKKGRVYYVGTSLEPASFVHFFRKVLIREKLPFRFLGKNIELIRR
                     KGKKSDYEFLLNHSGNSAFAGLERIKPYEFKTRKLEKR"
BASE COUNT          248 a          222 c          200 g          355 t
ORIGIN      
        1 taaacagtat gatccttttt ttcatgcgct gtgtatgacc gcagaaaacc gttgaggtag
       61 gcagaatcat caaggttcat ttatcttttc tccaattttc tggttttgaa ttcgtaaggt
      121 tttattctct ctaaaccggc aaacgctgaa tttccgctgt ggttcagcag gaattcataa
      181 tcagattttt ttccttttct tctgatcagc tcaatgttct ttcccaaaaa tcggaaaggg
      241 agtttttcac ggataagtac ttttctgaaa aaatgcacaa aggaggcagg ttcgagagag
      301 gttccgacat aatatactct tcctttctta taccgattga ctgtaattgc gggtctgccg
      361 tgataaaact ttttttttgc cctgtacctg gcaagtacct gggcggtatc cggttccagt
      421 atttccgcta tttttcctgc cttatgactc agtattccga aactgatgtt taatgtattt
      481 tttcctaatg attcaaattt tctgattttg attcccgcaa gatccttgaa cggtcccgga
      541 ggaacatcat ctgtcatcca tccgtttttc tgttttgtgc ctgaccggta gcctaataca
      601 agtattccgc cgtccgcgac aaagttttca agtctcttta caaattcggg atctgtctga
      661 atgtacaatg gcagaacgat cattctgtat ttcttcagat cagtttcgga tgccggaagt
      721 atatgagtcg gcacattcag tatattcagg cctgcgtacc atgtgaagaa ttccttgtcg
      781 aatccaatgt ccagaaaatc attcactttc tcagtcaggc cttctgaaat aggctgatat
      841 ttcaggtttc ttacattttc gatatcgtag agaacagcag tttctgcagt gaaatcttct
      901 gaaatgaaat catccgcagt ctcattcagt ctgctgactg tcttctgaag ttccaggtat
      961 cggtctgtaa tgcctttatc atgatccagt attccgtaac agagctgttc ctgtcctgtt
     1021 ctggc
//
LOCUS       DAOQGJ010001976         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_130911_length_1025_cov_2.209278, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001976 DAOQGJ010000000
VERSION     DAOQGJ010001976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130911_length_1025_cov_2.209278"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PK683_23020"
     CDS             complement(<1..918)
                     /locus_tag="PK683_23020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH11391.1"
                     /translation="MKRDIADIIKSVIIIIAVYFLQNAFWPLIRPYVWFLFYPAVFLS
                     CFAGGRYGGYVSSVLSAFLVSVNFIPSSGFLVFVNPKDWASVTVFTLTGFLFAYFVNR
                     YFAVLQESKESLERRIQERTSELQQSEERYKDLVEGSPAVLYIFSTVKGGLYYSQNAS
                     EIFSCTVEFLLENPFHWNQSILAEDAEIINSAVSGGQSFSVEYRIRDAQGRVRWIHDR
                     SVSRTVKNNEIIIKGLAVDITERKIAEQSLLEEKKKWHNLLESLPELIWTADPSGNWD
                     FLSSQWVGYTGVSEKDQTGYGWLNQVFEED"
BASE COUNT          308 a          238 c          202 g          277 t
ORIGIN      
        1 gtcttcttca aaaacctgat tcagccatcc gtaccccgtc tgatcctttt ctgagacacc
       61 tgtataaccg acccattgcg agcttaggaa atcccagttt ccggacggat cggcagtcca
      121 gataagttca gggagagatt cgagtagatt atgccatttt ttcttttctt ccagaagaga
      181 ctgttcggca attttgcgtt ctgtaatgtc gacagcgaga cccttaatga taatttcatt
      241 attttttact gtcctgctga cagatctgtc atgaatccac ctgacccttc cttgggcatc
      301 cctgattctg tattcgacac tgaaggactg tcctcctgaa actgctgaat taattatttc
      361 tgcatcttca gcaagtatgg actgattcca gtgaaacggg ttttcaagca gaaactctac
      421 tgtgcatgaa aagatttcag aagcattctg cgaataataa agacctccct ttactgtgga
      481 gaaaatgtaa agaacggccg gcgagccttc aaccagatcc ttgtatcttt cctcgctctg
      541 ctgaagctca gatgttcttt cctgaattct cctttcaaga ctttcctttg actcctgaag
      601 cacagcaaaa tacctgttta caaaataggc aaaaaggaac ccggtcagag taaacactgt
      661 tacgcttgcc cagtccttag ggtttacgaa tacgagaaaa ccggaagagg ggataaaatt
      721 caccgaaaca agaaatgcag aaagaacgga tgaaacatag cctccgtatc tgcctccggc
      781 aaaacatgac agaaagactg cgggatagaa caggaaccag acataaggac ggatcaaagg
      841 ccagaaggca ttctgtaaaa aatatactgc tatgataata attacacttt taataatatc
      901 agcaatatct cttttcatcc tgaaaatgtc atttctccgg ttttctccct gtttccccag
      961 gcatatcaga ccggaaataa tgcggcctaa ttgataaaat ggcaggcatc agagaaacga
     1021 caagc
//
LOCUS       DAOQGJ010001977         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131024_length_1024_cov_3.784314, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001977 DAOQGJ010000000
VERSION     DAOQGJ010001977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131024_length_1024_cov_3.784314"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK683_23025"
     CDS             complement(<1..268)
                     /locus_tag="PK683_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11392.1"
                     /translation="MALSTEQIIELQKYQKMLYQLEKILLVSGNEDQKKRVSKTIQKY
                     KSRILSISPDGIPDEVLVARPTVKTSVESKSSGSNDPSKQVVTSV"
     gene            complement(278..>1024)
                     /locus_tag="PK683_23030"
     CDS             complement(278..>1024)
                     /locus_tag="PK683_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HNH11393.1"
                     /translation="KVTYVHTLKEQAIEVQPQICITKDNVQVKVDGVLYLKILDPQKA
                     SYGIEDYKFAAIQLAQTTMRAVIGMMELDKTFEARETINHRIVDVIEQAAEPWGVRVN
                     RYEIQNLTPPKTVLDSMEKQKTAELNKKAQISLSEGEKFSRINRSEGQKEEAINKSLG
                     EKQKKINEAEGKAKEIEAIALATAKGIEAVADSVSKKGGNEAVRLQITQTFIKEFGHL
                     AKNTTEVLLPLDLTDINSVTESLNNLKQEG"
BASE COUNT          225 a          238 c          205 g          356 t
ORIGIN      
        1 gaacagatgt gactacctgc ttggacggat catttgagcc ggaggatttt gattcaacgg
       61 aagtctttac agtcggtctt gcaacaagaa cctcatccgg aatcccgtca ggagaaatgc
      121 tgagaattct tgacttgtat ttctgaatcg tcttgctgac ccttttcttc tgatcttcgt
      181 ttccggagac cagaagaatt ttttccagct gatacagcat tttctgatat ttctgaagtt
      241 ctatgatctg ttcagtgctc agagccattt tgtattatca gccttcctgt ttcagattgt
      301 tcagtgattc agtgaccgaa ttgatgtcag tgagatccaa aggaagaaga acttccgtgg
      361 tgttcttggc cagatgaccg aattccttga tgaatgtctg agtgatctgc agtctgactg
      421 cttcattgcc tcccttcttg gagactgaat ctgccactgc ttcaattccc tttgcagtcg
      481 caagtgcaat tgcttcaatt tcctttgcct ttccttctgc ttcattgatc tttttctgtt
      541 tttcaccgag agatttgttg atagcctctt ctttctgtcc ttctgaacgg ttgattctcg
      601 aaaacttttc tccttctgaa agggaaatct gagccttctt attcagttcg gcagtcttct
      661 gtttttccat tgaatcgaga acagtcttgg gaggagtgag attctgaatt tcatatcggt
      721 tgactcttac gccccaaggt tcagcagcct gttcaattac atccacgatt ctatggttga
      781 tcgtctctct ggcttcaaat gtcttgtcca gttccatcat cccgatcact gccctcattg
      841 ttgtctgagc cagctgaatt gcagcaaact tatagtcctc aatcccgtaa ctggctttct
      901 gaggatccaa tattttcaga tacagaactc cgtccacttt tacctggaca ttgtcctttg
      961 taatgcagat ctgaggctga acctcaattg cctgttcttt cagagtatga acataagtga
     1021 cttt
//
LOCUS       DAOQGJ010001978         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131167_length_1024_cov_1.969040, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001978 DAOQGJ010000000
VERSION     DAOQGJ010001978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131167_length_1024_cov_1.969040"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1024)
                     /locus_tag="PK683_23035"
     CDS             complement(<1..>1024)
                     /locus_tag="PK683_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11394.1"
                     /translation="CSGIKKKAEEKWKAVAAYLFLNKAPSKVSMNISLVGADGKSITV
                     GNTSVREASSVSSESEKPSENSSAGAPGSAGQKAGGETLNLDLNGAPKAVEVLDSNGN
                     YAGTITVTPRTGDETYTNSFLSFALRSDSEPAAGAVQGVITGEVIITEVPFQDLTKLS
                     AVFTIDGEKVRLEGADQVTGKTVNDFSQNRTFTVTAKNGTEKKYTVITKKKYYTCELA
                     GLYFTDYPLTAVRQNSDASSVWYEAVLPPDADVSSLKTSFSVYGTSVKIGSNVQISGQ
                     SLNSFSSPVTFTVSGSNGCTADYTVKVSKENLAAGKNLKKGRKLTRSELELVDFPEIT
                     KIGAVGI"
BASE COUNT          238 a          248 c          233 g          305 t
ORIGIN      
        1 gtataccgac tgcaccgatt tttgtgattt ccgggaagtc cacaagttcc agttcgcttc
       61 gagtcagctt cctccccttc ttcagatttt ttcctgcagc aagattctcc ttggacacct
      121 tgactgtata gtctgctgtg cacccgttgg aaccgctgac agtgaatgtg acaggagagg
      181 aaaaactgtt cagagactgt ccggaaatct gcacattgga tccgattttg accgaggttc
      241 cgtaaacgga aaatgaagtc ttcagtgagg aaacgtcagc atcgggagga agtacagcct
      301 cgtaccaaac agaagaagca tctgaattct gacgcactgc agtcaaaggg taatctgtaa
      361 aataaagacc tgcaagctcg catgtataat atttcttttt tgtaattaca gtgtattttt
      421 tttccgttcc gttttttgca gtgactgtaa aggttctgtt ctgagagaaa tcattgactg
      481 tttttcctgt gacttgatct gccccctcca gtctgacttt ttccccgtca attgtaaaca
      541 ctgcggaaag tttcgtaaga tcctgaaacg gaacttctgt tatgatgacc tcgcctgtaa
      601 tgactccctg aaccgctccg gctgcaggtt ctgaatcact tctgagtgca aaggaaagga
      661 aggagtttgt ataagtttca tctcctgttc tgggagtcac tgtaattgtg cctgcatagt
      721 ttccgttgga atccagaacc tccacagcct tgggagcgcc gttcagatcc agattcagag
      781 tttcaccgcc tgccttctgt cctgcagaac cgggtgcacc tgcagaagaa ttttcagagg
      841 gtttttcgga ttcgctgctg actgaggaag cttcccgcac cgaggtgttt cccactgtta
      901 tggatttccc gtctgcaccg accagactga tgttcatgct gaccttcgag ggggctttgt
      961 tcagaaacag atacgctgcg acagccttcc atttttcttc agcctttttc tttatccctg
     1021 aaca
//
LOCUS       DAOQGJ010001979         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131296_length_1023_cov_2.825413, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001979 DAOQGJ010000000
VERSION     DAOQGJ010001979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131296_length_1023_cov_2.825413"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PK683_23040"
     CDS             <1..634
                     /locus_tag="PK683_23040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11395.1"
                     /translation="ICLADHLKRYPLQETLERAFPGSDVVPLSEVTEGQAITVSLGLK
                     QEDYDYPLLIAASDNGMLYDWSRFESLAEDPKTDMIVFTFSGHPGSARNPKMYGWVKK
                     NGNRAAGISVKVPISENPIQDDAVVGAFYFKSAGLFMDILSEMKEKNIRVNGEFYADS
                     MAGIAVERGLNVQVLRVDHYICWGTPDEYNTYQYWKEFFSKVSWHPYGKK"
     gene            631..996
                     /locus_tag="PK683_23045"
     CDS             631..996
                     /locus_tag="PK683_23045"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNH11396.1"
                     /translation="MKREILWFLFSGFSAVGVDLCTYYTLLNWLDKSPAKAVSFVTGS
                     VAAFILNKYLTFQKKDESAADEIWKFIILYSSTLGANVAVNKIVLLVFPEFTFFAFLC
                     ATGTSTILNYIGQKFWVFK"
BASE COUNT          290 a          183 c          230 g          320 t
ORIGIN      
        1 tatctgtctt gctgatcatc tgaagcgata tcctcttcag gaaacacttg agagagcttt
       61 tccgggatct gacgttgtac ctctcagcga agtgaccgaa ggtcaggcaa ttactgtttc
      121 tcttggactg aaacaggaag attatgatta tccgcttctg attgcagcca gtgataatgg
      181 aatgctttat gactggagca gatttgagtc acttgcagaa gaccctaaaa cagatatgat
      241 tgtatttaca ttcagcggac atccgggaag tgcaagaaat ccgaagatgt acggctgggt
      301 gaaaaaaaac ggaaacaggg ctgcagggat ttcagtgaaa gttccgattt ctgaaaatcc
      361 gattcaggat gatgcagttg tcggtgcgtt ttattttaaa tcagccgggc tttttatgga
      421 tattctttct gaaatgaaag agaagaatat ccgtgtaaac ggagagtttt atgctgattc
      481 aatggcggga attgctgtag agcggggact gaatgttcag gtgctgagag tagatcatta
      541 tatctgctgg ggaactcctg atgaatacaa tacttatcag tattggaaag aatttttctc
      601 caaagtttcc tggcatcctt acgggaaaaa atgaaaagag agattctatg gtttcttttc
      661 tcgggatttt ccgcagtagg agttgatctg tgcacatatt atactcttct caattggctc
      721 gacaagtctc ctgcaaaagc agtttcattt gtcaccggtt ctgttgccgc cttcattctg
      781 aataaatatc tgacatttca aaaaaaagat gaatcagctg cagatgaaat ctggaaattc
      841 atcattctct attcatccac actgggagca aatgttgcag tcaataaaat tgtgctgctt
      901 gtttttccgg aatttacttt ttttgctttt ctttgtgcta caggcacttc aacgattttg
      961 aattatatag gtcaaaagtt ctgggtattc aaatgacgaa actgacaata gtgattccct
     1021 gct
//
LOCUS       DAOQGJ010001980         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131388_length_1023_cov_2.022727, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001980 DAOQGJ010000000
VERSION     DAOQGJ010001980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131388_length_1023_cov_2.022727"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..868
                     /locus_tag="PK683_23050"
     CDS             194..868
                     /locus_tag="PK683_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002107968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNH11397.1"
                     /translation="MESFKRTADIEALQVPGDKDGLAVILIHGFGANAMDLYPLKNYI
                     QLPKGTNWYFPNGIQDVPISPQYSGKAWFPIGISTLLQDGDFSKVAEYEPPGLNEAFN
                     KMDAFIRALPHPYEKIILGGFSQGAMLSTELFLRREERPHSLILMSGTLLNTNRWKKA
                     AEKKKGYQFFQSHGTNDPLLPFSAAKKLESIFREAGLIGEFVQFQGGHEIPEQVLKRA
                     SAYLKR"
BASE COUNT          303 a          227 c          223 g          270 t
ORIGIN      
        1 cttcttctac atcaaggcag gatttgacat agtcggaaca attgaagact atccggcagg
       61 ttccaactgc tactttctga aaaagaatat gccgaaaaat gcatcaccgt catgatttta
      121 tatccttttt taataaaaaa taacagtcaa ctatattata aatatttata tatattttga
      181 ttcagaggca gagatggaaa gctttaaaag aacagcagac attgaggcac tgcaggtgcc
      241 tggcgataag gacggtcttg ccgtcatact cattcacgga ttcggggcga atgcaatgga
      301 tctctatccc ctgaaaaatt atattcagct gcccaagggc acaaactggt attttccaaa
      361 cggaattcaa gatgtgccga tcagtcctca gtattcggga aaggcctggt ttcccatcgg
      421 catctcaacc ctgcttcagg acggtgattt ttccaaggtc gccgaatatg aacctcctgg
      481 actgaatgag gctttcaaca agatggatgc tttcatccga gcactccccc atccctatga
      541 aaaaatcata ctgggcggat tcagtcaggg tgcaatgctt tccacagaac tttttctgag
      601 aagagaagag cgtcctcaca gtctgatcct gatgtcagga acccttctga atacaaaccg
      661 ctggaaaaaa gcagcagaga aaaagaaagg ttaccagttc tttcagtcgc acggaacaaa
      721 tgatcctctc ctcccctttt ctgctgccaa aaaactggaa tccattttcc gcgaagccgg
      781 actgatcggt gaatttgttc agtttcaagg cggccacgag attccggagc aggttttaaa
      841 aagagcatct gcgtatttga agagatagtc tgttaaaggg atcagggatc agggagcgga
      901 gagcggagtt caggacttga cactgaaata taatatatgc ttttctgtca gattcatatt
      961 cttctttttt gggcgaatcg cacagcctct caaacagcat atctttccga aaaagtgcga
     1021 ccg
//
LOCUS       DAOQGJ010001981         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131411_length_1023_cov_1.878099, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001981 DAOQGJ010000000
VERSION     DAOQGJ010001981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131411_length_1023_cov_1.878099"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="PK683_23055"
     CDS             <1..>1023
                     /locus_tag="PK683_23055"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNH11398.1"
                     /translation="VLLAALQPGGYLLATGFWFILLYASGIEGRAFSVLGFILKAVFS
                     YAFIFLVSRLCDVVTLFLKNRAEKTKSVLDEQLVPLLSRTIKLTAVTVTVLLCVQNMG
                     INVLSILAGLGIGGLAIALAAKDTAANLFGSVMIFLDKPFLVGDHVIIGSSEGSVEEI
                     GFRSTRLRTWDDSIVSIPNSTVASANVNNMGMRRCRRTNVTLNVTYDTSSLKMEAFLE
                     GIKNILRKRPVVHQDTMIVGFSTMNSSSLDIIMHFYADTKDWAEDIELRQNIYIEIMH
                     LAEETGVSFAYPSQSLYVETFPEKEKNVKSNLKSEEELAETAANFGGQGKFSRPNGMG
                     IFTPPFR"
BASE COUNT          277 a          234 c          245 g          267 t
ORIGIN      
        1 gtgctgctcg ctgctctgca gccgggaggt tatctgcttg cgacaggttt ctggttcatt
       61 cttctgtatg cttcaggaat cgagggccgt gcattttcag tgctcggttt cattctgaaa
      121 gcagttttca gttatgcgtt tattttcctc gtttcaagac tctgcgatgt agtcacgctt
      181 ttcctgaaaa acagggcaga gaagaccaaa agcgttctgg atgagcagct ggttcctctt
      241 ctgagccgca caataaaact cactgcagtc actgtgactg ttcttctctg cgttcagaat
      301 atgggaatca atgtgctttc gattctcgca ggactcggga tcggaggtct agcaattgct
      361 cttgcggcaa aggacacagc ggccaatctt ttcggttcag tgatgatctt cctcgacaaa
      421 ccttttcttg tgggagacca tgtcatcatc ggctcctccg aaggatcagt cgaagaaata
      481 ggattcagat cgactagact cagaacctgg gacgattcga ttgtatcgat accaaactcc
      541 actgtcgcat ccgcaaacgt caacaatatg ggaatgagac ggtgcagaag aaccaacgtc
      601 acactgaatg taacgtacga tacctcctct ctgaaaatgg aggccttcct cgaaggaata
      661 aaaaacatcc tgaggaaacg gcctgttgtg catcaggata caatgattgt aggattcagc
      721 actatgaaca gttcgagcct ggacattatc atgcattttt atgcagacac gaaggactgg
      781 gcagaggaca tagaactccg tcagaacata tacattgaaa tcatgcatct ggctgaggaa
      841 accggtgttt cgtttgcata tccttcacag tctctctatg tggaaacatt tccggaaaag
      901 gaaaagaatg taaaatccaa tctcaaatcg gaagaggaac ttgctgagac ggcagcaaat
      961 ttcggagggc agggaaaatt ctccagaccg aacggaatgg gaatattcac tcctcctttc
     1021 cgg
//
LOCUS       DAOQGJ010001982         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131459_length_1023_cov_1.334711, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001982 DAOQGJ010000000
VERSION     DAOQGJ010001982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131459_length_1023_cov_1.334711"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..828
                     /locus_tag="PK683_23060"
     CDS             55..828
                     /locus_tag="PK683_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNH11399.1"
                     /translation="MDIPYYSPVGHETEIFEHAYRNHLPLLLKGPTGTGKTRFISHMA
                     AKLGQKLVTVSCHEETSAIDLLGRYLIKGTETVWQDGPLTHAVREGYIIYIDEIAEAR
                     PDTIVILHSLTDSRRVLFNERTNEEIPAKDSFMLTASFNPEYQGSWKELKPSTRQRFI
                     SIQFNYPRPKEETEIILHESGVERKTAEKLVELAGKVRSMKELSLSETISTRLLVDAA
                     KLIRSGLSPRLSCETGIIEPLTDDRDTASALKDIISLYI"
     gene            828..>1023
                     /locus_tag="PK683_23065"
     CDS             828..>1023
                     /locus_tag="PK683_23065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11400.1"
                     /translation="MEIGEKIFQKTADLIERIRTAGLRKNRIPLNLEMHKSSLTVFAR
                     ILSGKPVNLHRAENFGGLSGL"
BASE COUNT          315 a          231 c          239 g          238 t
ORIGIN      
        1 acttcccaat gatgaaatgc tgaatgcaga cgattcagag ttttccgcat cctgatggac
       61 attccatact acagtccagt cggacatgag acagaaatct ttgagcacgc atacaggaat
      121 catcttccgc tcctgctgaa aggtccgacc ggaactggaa agacaagatt catttctcat
      181 atggctgcaa aactcggtca gaaactcgtg acagtgagct gtcatgagga gacttctgcg
      241 attgatcttc tgggaaggta tctcatcaag ggaacggaaa cagtctggca ggacgggcct
      301 ctgactcatg cagtgagaga aggatatatt atctatatag acgaaattgc agaagcaaga
      361 ccggatacga tagtgattct tcattcactg actgattcca gaagggtact tttcaatgag
      421 agaacaaatg aagaaattcc cgcaaaggac agttttatgc tgacagcctc gttcaatcct
      481 gagtatcagg gaagctggaa ggaactgaaa ccgtccacaa gacagagatt tatttccata
      541 cagtttaatt atcccagacc gaaggaggaa acagagatca ttctgcatga atccggagtg
      601 gaaagaaaga ccgcagagaa acttgtagaa cttgccggaa aggtgcgttc catgaaggag
      661 ctatccctct ctgagacaat ctccacaagg cttctggtcg atgctgccaa actcatccga
      721 agcgggcttt ctccgcgtct ctcctgtgaa acaggaatca ttgaacctct gactgatgac
      781 agagacactg cttctgcact caaagacatc atcagtctgt acatctgatg gaaatcggcg
      841 aaaaaatatt tcagaagact gcagatctga ttgaaagaat cagaactgca ggactccgga
      901 aaaaccgtat acctctgaat ctggaaatgc ataaatccag tctcactgtt tttgcccgaa
      961 ttctctccgg gaaaccggtc aatctgcaca gagctgagaa cttcggagga ctgagcggtc
     1021 tga
//
LOCUS       DAOQGJ010001983         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131763_length_1021_cov_3.139752, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001983 DAOQGJ010000000
VERSION     DAOQGJ010001983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131763_length_1021_cov_3.139752"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..744
                     /locus_tag="PK683_23070"
     CDS             133..744
                     /locus_tag="PK683_23070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH11401.1"
                     /translation="MYKYLFSRVYDPFMRSLEKKELFRRRRRLLKDLTGSVLEIGSGT
                     GVNFPFYRNGVKVLAVEPNVHMWDIINKKIMQNKYECEIIPAVSSIEKLESEDTIPNS
                     SLDAAVCTLVLCNVPDAEKTLRFIFQKLKKGGKLIMIEHIRSHHSLTSKFQDIAAPAW
                     RILADGCCLNRATDQMPEKIGFTKKEETYFSTVLPFYQAVWER"
     gene            complement(746..>1021)
                     /locus_tag="PK683_23075"
     CDS             complement(746..>1021)
                     /locus_tag="PK683_23075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11402.1"
                     /translation="GSPFRIRKNEPGKITVPLFAVFGKTDGISPEETVYPCFYPEKRS
                     RDSKAEFLEASTANDLSMDYDHFDLFFSEKAESEIYKKITDWMDSLK"
BASE COUNT          309 a          202 c          226 g          284 t
ORIGIN      
        1 caaggacgat atgaggattc ttgaaactgt cggcattccc gatcagaaga tgacattcaa
       61 gcagaacgac atcatggtaa ttttcggaag ccatgaagat gtggaaaaat tcctgaattc
      121 aatttaaccg ccgtgtataa atatcttttt tcaagagtct atgatccttt tatgaggtcg
      181 ctggagaaaa aggaactgtt caggagacgc agacggctct tgaaggacct taccggatcc
      241 gttctggaaa taggaagcgg aaccggggtc aattttcctt tttacagaaa cggcgtcaaa
      301 gtactggcag tcgagccgaa tgtgcatatg tgggatatta ttaataaaaa aataatgcag
      361 aataaatatg aatgcgaaat tattcccgca gtgtccagca ttgaaaaact ggagagtgaa
      421 gatacgattc cgaattccag tcttgatgca gcggtatgca cacttgtcct ctgcaatgtg
      481 ccagatgcag aaaaaactct ccgtttcata tttcagaaac tgaagaaagg cgggaagctg
      541 atcatgatag aacatatcag atctcatcat tccctgactt caaaatttca ggacatagcc
      601 gcgccggcat ggaggattct ggcagacgga tgctgtctca acagggcaac tgatcagatg
      661 cctgaaaaga tcggattcac taaaaaagaa gaaacatatt tcagtacagt tcttcctttt
      721 tatcaggcag tatgggaaag gtgattcatt ttaagctgtc catccagtct gtaatttttt
      781 tgtaaatctc tgactcggcc ttttctgaaa agaaaagatc gaaatgatcg tagtccatcg
      841 agagatcatt tgcagtgctt gcctccagaa attctgcctt ggaatctctg cttctttttt
      901 ccggataaaa acacggatat acagtttctt caggactgat tccgtctgtt tttccgaaga
      961 ctgcaaaaag aggaaccgtg atttttcccg gttcattctt tctgattctg aacggggaac
     1021 c
//
LOCUS       DAOQGJ010001984         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131840_length_1021_cov_2.272257, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001984 DAOQGJ010000000
VERSION     DAOQGJ010001984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131840_length_1021_cov_2.272257"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PK683_23080"
     CDS             complement(<1..1013)
                     /locus_tag="PK683_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11403.1"
                     /translation="METRLLKCREINQSFLDRWPIEAVRKMTLEEYVDTGNPDTFCQW
                     IETQTIKLGSIKGQFSNKFGIYKRNNPAERPETLTSDEAYTWIPRYGDDRNTAYRNVR
                     DEIVQIIIAAQAGDFSAIDSIRTNHIMKWKIAFLYSQERLVPIFKNDVLKAIAEFQGM
                     DIHKKETSELQHFILSNKPLSQNIYQYASELFRQFGKDDEQNPSYYLIGSKYGENAHE
                     DMFPLMEEFSVVCTGFAWGTDLSYLYREKEAEIIRELKALGEESKSINALKQFLQLKP
                     GDIIAVKSTGNPKAGNPFLEIIAYAVVVEREGKTYYHETENFGHCINVEFIKTGLNNT
                     FSVG"
BASE COUNT          283 a          195 c          176 g          367 t
ORIGIN      
        1 ccaacggaaa acgtattatt caaaccagtc tttataaatt ccacattgat gcagtgtccg
       61 aagttttcag tttcgtggta ataggttttg ccttctcttt caacaacaac tgcataggca
      121 atgatttcaa gaaacggatt tcctgctttg ggatttccag tagatttgac tgcgattata
      181 tcgccgggtt ttagctggag gaattgttta agagcgttaa tggatttact ttcttctccc
      241 agtgctttta gttctcgtat aatctcagct tctttttccc tgtacaaata tgaaaggtct
      301 gttccccatg caaatccggt gcaaacaaca ctgaattctt ccataagagg aaacatgtct
      361 tcatgagcat tctctccata ttttgatcca atcagataat aagaaggatt ttgttcatcg
      421 tcttttccaa attgccggaa taactcggaa gcgtattgat aaatattctg actgagaggt
      481 ttattactaa gtatgaaatg ctgaagttca gatgtttctt tcttatgaat atccattccc
      541 tgaaattcag caatggcttt aagtacatcg tttttaaaaa taggaacaag tctttcctgt
      601 gaataaagaa atgctatttt ccatttcatt atgtgattgg ttcttatgga atcaatagca
      661 gaaaaatctc cggcctgtgc agcaataata atttgtacaa tttcatctct tacatttctg
      721 taagcagtgt ttctgtcatc accataacgt ggaatccagg tatatgcttc atcggaagtt
      781 aaagtctcag gccgttcagc aggattattt cttttgtaaa tcccaaattt atttgagaac
      841 tgtcctttta tacttccaag ttttattgtt tgtgtttcta tccactggca aaacgtatca
      901 ggattaccag tatcgacata ctcttcaagc gtcatttttc ttactgcttc aatcggccat
      961 ctgtcaagaa aggactgatt tatttctctg catttcagaa gtcgtgtttc cataattttt
     1021 g
//
LOCUS       DAOQGJ010001985         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131892_length_1021_cov_1.957557, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001985 DAOQGJ010000000
VERSION     DAOQGJ010001985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131892_length_1021_cov_1.957557"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PK683_23085"
     CDS             complement(<1..642)
                     /locus_tag="PK683_23085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH11404.1"
                     /translation="MIRFIWTYTVTFFYILKLKRKLGADFFQNAEQGTRVADKAIEAA
                     GYTVKIIGEENIPAENGVLYISNHQSFFDIFASIHILRRQLGFIAKAQLRNFFYVGHY
                     VEKMGGVLIDRDDVRSQVEMISQLTKTLKSGYNVFIFPEGTRSRDGKLSEFKSGSFRT
                     ALKSRCRIVPLTFFNNHEVAANRGDTVIKVKIDPPVGPELYEKMNTSEISDYVK"
BASE COUNT          276 a          226 c          203 g          316 t
ORIGIN      
        1 ttttacgtaa tcgctgattt cggaagtgtt cattttttca taaagctccg ggccgacagg
       61 aggatctatt tttaccttga tcacagtgtc tcctctgttt gcggctactt catgattatt
      121 gaaaaaggtc agaggaacga tcctgcatct gctcttcagt gcagtcctga agcttccgct
      181 cttgaactca gagagttttc cgtctctgct tcttgtgccc tccggaaaaa taaatacatt
      241 gtatccggac ttcagagttt tggtcagctg ggatatcatc tccacctgac ttcttacatc
      301 atccctgtca atcagtactc cgcccatctt ttccacataa tgacctacat aaaagaagtt
      361 tctcagctgt gcctttgcaa tgaacccgag ctgtctgcgg aggatatgga tggaggcgaa
      421 aatgtcaaaa aaagactgat gattggaaat atagaggact ccgttttccg ccgggatatt
      481 ttcttctccg atgatcttta cagtgtatcc cgcagcctca atcgctttgt ctgccacccg
      541 tgttccctgt tccgcatttt gaaaaaagtc tgctcccagt tttcttttca gtttcagaat
      601 ataaaaaaat gtgactgtat aagtccatat aaatcgaatc atagtgtttc tcctgatgca
      661 gctcagaccg ggtactgcag actctgcagc cgattcagga caagcttatt tctctgatct
      721 tcagatgcta cataaaaaaa cggatattcg gctttccctt ctgcacaggc ctgcttccga
      781 ttctgaaacc ggataaaacc tgcagagtaa cagtttatat aagatgagag ataattgaga
      841 ctccttatca gttgactttt gtaatgtaaa aattaatatg gatactgttt tcacggtcgt
      901 atccggatat tttcagagac actgtatctt gccttaattc agtatcagag cggcagggaa
      961 acaaattctg catggaaaat ttgatattag agcataaact gctctgagga aaagaaatga
     1021 c
//
LOCUS       DAOQGJ010001986         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131979_length_1020_cov_3.561658, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001986 DAOQGJ010000000
VERSION     DAOQGJ010001986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131979_length_1020_cov_3.561658"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PK683_23090"
     CDS             <1..654
                     /locus_tag="PK683_23090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH11405.1"
                     /translation="DRNFRLALNHSIDRKKIMDRLFLGLAEDTVYSFPKKFASGLPEM
                     KFEYSPELAGNYLKKSACYPEILQKPLSFRMRADDENKAKGPVILQYLKDLGLKVELE
                     MMEKAALYRENAEGKGDITLLTWYSDNDESIDFIDPVFSSKSLGGGGNRSFFENEEMQ
                     KIIEVSAKSGTLSLQNRIRAAEIQEEKLPWIYLWSLHENYLISDKLRNSPSAGGFIF"
     gene            651..>1020
                     /locus_tag="PK683_23095"
     CDS             651..>1020
                     /locus_tag="PK683_23095"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNH11406.1"
                     /translation="MNEDILKISLPKPDEVLKENLKRKMDSKTKPLGSLGRLEDAAVK
                     IGSIFGTETPEILNPTALIFASDHGIAEEGISAYPQEVTYQMVMNFLRGGAAVNVLCR
                     ENGIRLVLTDSGVRFKFAPDP"
BASE COUNT          316 a          195 c          257 g          252 t
ORIGIN      
        1 gacagaaact tccggctggc actcaatcat tcaatagaca gaaaaaagat catggacagg
       61 ctgtttctgg ggcttgctga ggatacagtg tattcctttc cgaagaaatt tgcatcaggt
      121 cttcctgaaa tgaaatttga atacagtcct gaactggcag gaaattatct gaaaaagtct
      181 gcctgttatc ctgaaatact gcagaagccg ctgagctttc gaatgagggc agacgatgaa
      241 aacaaggcca agggtccagt cattcttcag tatctgaagg atcttggact gaaggttgag
      301 ctggagatga tggaaaaagc tgccctttac agagaaaacg cggaagggaa gggtgacatt
      361 acacttctga cctggtactc tgataatgat gaatcaatag attttataga tcctgtattc
      421 tcttcaaaaa gtcttggcgg aggaggaaac agaagttttt ttgaaaacga ggagatgcag
      481 aaaatcattg aggtttctgc aaaatcggga accttgagtc ttcagaacag aatccgggcg
      541 gcagagattc aggaggagaa acttccatgg atttatctct ggtcccttca tgaaaattat
      601 ctgatttcag acaagctgag aaacagtccg agtgcaggag gatttatatt ttgaatgaag
      661 atatactgaa aatatcacta ccgaagccgg atgaagttct caaggaaaac ctgaaaagaa
      721 aaatggacag caagacgaaa cctctcggtt ctctcggaag gctggaggat gctgcagtga
      781 aaattggaag catattcgga acagaaactc cggagattct gaatcccaca gctctgatct
      841 ttgcttccga tcatgggatt gcagaagaag gaatcagtgc ctatcctcag gaagtcactt
      901 accagatggt gatgaatttt ctgcgcgggg gagctgcagt caatgtgctc tgcagagaaa
      961 acggaatccg tcttgttctg acagattcag gggtcaggtt caaatttgcc ccggaccctc
//
LOCUS       DAOQGJ010001987         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_131999_length_1020_cov_3.140933, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001987 DAOQGJ010000000
VERSION     DAOQGJ010001987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131999_length_1020_cov_3.140933"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK683_23100"
     CDS             <1..396
                     /locus_tag="PK683_23100"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH11407.1"
                     /translation="PEERVQIVQPSSTKTDFIKPPEIHTDSLKNNHKENSSEPPVNTS
                     DVKETKIPLKEKDSVKTLTVTVNTVTEKKKRFPEKIKVKKGESLRSIAEAVFGKESLW
                     KKIYKINRTKIKNPNFIKAGEILDLPKVK"
     gene            522..752
                     /locus_tag="PK683_23105"
     CDS             522..752
                     /locus_tag="PK683_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004468351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNH11408.1"
                     /translation="MKQFNRITMNPNQMGGKPCIRGMRVTVGVILGLLASDKSVEQIL
                     VAYPYLEKEDIQESLEYAAWRMMEFEAEIPAA"
     gene            749..>1020
                     /locus_tag="PK683_23110"
     CDS             749..>1020
                     /locus_tag="PK683_23110"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNH11409.1"
                     /translation="MRFLIDMNLSPQWVDFLKEFNFESRHWSQTGDIRASDKEIFDWS
                     RENDFIIIIQDLDFSTLLALSNSEKPSVILIRTKNSLPEQFGQILI"
BASE COUNT          368 a          182 c          208 g          262 t
ORIGIN      
        1 cccgaagaga gagttcagat tgttcagcct tcaagcacaa agacagactt tataaagcct
       61 cctgaaatac atacagattc attaaaaaat aatcataaag aaaacagttc agagccgccc
      121 gtcaatacga gcgatgtaaa agaaaccaag attcctttga aggaaaagga ttctgtaaaa
      181 acactgactg tcacagtcaa tacagttacg gaaaaaaaga aaaggtttcc tgaaaagata
      241 aaggtcaaaa aaggagaatc actccgttcc attgcagaag ctgtattcgg caaggaatct
      301 ctctggaaaa agatatataa aatcaaccgc actaaaatta aaaatccaaa cttcatcaaa
      361 gccggtgaaa ttctcgatct gccgaaagtg aaataaggaa aagtcaggaa agaaacttcc
      421 agatgaacag tgacagtatt gcttagtaaa gactcttttt actttccgaa aaagacttgc
      481 aaatccggct aatgactgca tttttgctga aggggaagtg tatgaaacaa tttaacagaa
      541 ttacaatgaa tccgaatcaa atgggcggaa aaccttgtat ccgcggaatg agagtgactg
      601 taggtgtgat tctaggactt ttggcatcag ataaatctgt tgagcagatc ctcgttgcat
      661 acccttatct ggaaaaggag gacatacagg agtcgttaga gtatgctgcc tggaggatga
      721 tggagtttga ggcagaaatt ccagctgcat gagattttta attgatatga atttgtcacc
      781 acagtgggta gattttctta aagagttcaa ttttgaatca cgtcattgga gtcaaacagg
      841 cgacataaga gcttctgata aggaaatatt tgactggtca agagaaaatg attttataat
      901 tataatacag gatttagact tcagcactct tttggctctg tcaaattctg aaaaacccag
      961 cgtaattctc atccgtacca aaaattcctt acccgagcag tttggccaga tactcatcaa
//
LOCUS       DAOQGJ010001988         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132030_length_1020_cov_2.671503, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001988 DAOQGJ010000000
VERSION     DAOQGJ010001988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132030_length_1020_cov_2.671503"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1020)
                     /gene="secY"
                     /locus_tag="PK683_23115"
     CDS             complement(<1..>1020)
                     /gene="secY"
                     /locus_tag="PK683_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNH11410.1"
                     /translation="LGIMPYISSSIVMQLVMVLFPQLQKLQKEGEEGRKKINQYTKYG
                     TIVLCAIQSSAVVLLARNWASQAGPTGLKYSNLIDPSMQSVFLPLGMLTITAGTMLLM
                     WMGEQITERGIGNGTSLIIFAGIVGRTPEAVAQLLTSKEADPLSVLILFVLFILLISF
                     TVILTQGVRKVPLHYGEQMQGRKMVQARSQSLPFKVNASNVMPIIFASSLILFPQTIF
                     STLSQNFTTWAGWAVVLDFFNPFSVVWYRAGFYYMIYSGLIIFFAYFYISILYNPGEL
                     AENLKKYGGFIPNVRPGSPTKEYIEKILNRITLPGAVFLSALTLAPYLIIKLLNIGVN
                     TGGGAL"
BASE COUNT          317 a          226 c          218 g          259 t
ORIGIN      
        1 cagagcaccg cctccggtat tcacaccgat attcagcagt ttaatgatca gataaggagc
       61 cagtgtaaga gctgaaagaa atactgctcc agggagagta attctgttca gaatcttctc
      121 aatatattcc ttagtaggag acccgggacg gacattcggg ataaacccgc cgtatttctt
      181 cagattctcc gccaattctc caggattata caatatagag atataaaagt atgcgaagaa
      241 tataatcagt ccagagtaga tcatataata gaatcctgct ctgtaccaga caaccgaaaa
      301 cgggttaaaa aagtccagaa ccactgccca tcctgcccaa gttgtaaagt tctgggaaag
      361 agtggaaaat attgtctgag ggaaaagaat taagctcgat gcaaaaatga tcggcataac
      421 gttcgatgca tttactttga atggaagact ctggcttctg gcctgaacca tctttctgcc
      481 ctgcatctgc tctccataat gaagaggaac cttgcggaca ccctgtgtga gtatgacagt
      541 gaaggagatc agaaggataa acagtacaaa taatatcaac acagataaag gatctgcttc
      601 ttttgaagta aggagctgtg caacagcttc aggagttctt ccgacaatac ctgcaaagat
      661 gatcagagat gttccgtttc caatccctct ttcggtgatc tgctcaccca tccacatcag
      721 gagcattgta cctgcagtga ttgtaagcat acccaacggg aggaagacac tctgcatact
      781 tggatcaatc agattggaat atttaagtcc tgtaggaccg gcctgactag cccagtttct
      841 tgcaagaaga accactgcag aactttggat agcacaaagc acaatcgttc catatttagt
      901 atactggttg attttctttc ttccctcttc cccttccttc tgaagcttct gaagctgagg
      961 gaagagaacc attacgagct gcataacgat cgaggaggaa atataaggca taatcccaag
//
LOCUS       DAOQGJ010001989         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132218_length_1019_cov_3.693983, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001989 DAOQGJ010000000
VERSION     DAOQGJ010001989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132218_length_1019_cov_3.693983"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PK683_23120"
     CDS             complement(<1..324)
                     /locus_tag="PK683_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11411.1"
                     /translation="MKFLSFIVSVLFLVSGCKDSKDFESMVEIVRMEKVRLDEKGEPL
                     VVDVEISYYACPGTQMEVIRGDKNFADCLYKSHKVGDKLKVHVNWYWDSFGYYKWKVT
                     QLNGCP"
     gene            complement(321..>1019)
                     /locus_tag="PK683_23125"
     CDS             complement(321..>1019)
                     /locus_tag="PK683_23125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11412.1"
                     /translation="GMVQDDDHIERIADEPTFKLWKFHALFYTQADLRAYPFDTQELE
                     IGMEEVDVGIDQINFIADQDHIRLDSDFVMPGWEVSHIESRVLTHYYPDRFEYDDLYY
                     PRFIFRLGIKRFANSALFTVFLPAFVIVLVSLSGIWLSGKEIETRINSSAPMLAAAVL
                     FHYTLSQEIPATAYLTRADKLMLGVYLGLITNLFATWAFFFFDPKYEERIFKLGRWIV
                     PPLNMILYTLGCII"
BASE COUNT          309 a          240 c          198 g          272 t
ORIGIN      
        1 aggacagccg ttcagctgag tcactttcca tttataatat ccgaaagaat cccagtacca
       61 gtttacatgg actttcagtt tatcccctac cttatgactt ttgtagagac agtctgcaaa
      121 atttttgtct cctctgatga cttccatctg ggttccaggg caggcatagt aggaaatctc
      181 cacgtcgacc acaagaggct ctcctttctc gtccagtcgg actttctcca tccgcacaat
      241 ctcgaccata gattcaaagt ccttgctgtc tttgcagccg gacaccagaa acagaacaga
      301 aacaataaat gacaggaatt tcatataatg caccctaatg tatacaaaat catattcaac
      361 ggaggaacaa tccacctgcc cagtttaaaa attctttcct cgtatttcgg atcaaaaaag
      421 aaaaatgccc atgtggcaaa cagatttgtg atgagcccca gatagactcc gagcatcagc
      481 ttgtctgctc ttgtcagata ggcagttgca ggtatttcct gggataaagt ataatgaaaa
      541 agcactgcag ccgcaagcat cggcgcgctg gaattgattc ttgtttcaat ttccttgcct
      601 gaaagccaga tccccgaaag agaaaccaga acaattacaa atgcaggaag aaatactgta
      661 aacagggctg aattggcaaa acgttttatt ccgagacgga aaatgaatcg gggatagtac
      721 agatcatcat attcaaatct gtccggataa taatgcgtga gaactcttga ctcgatatga
      781 gacacttccc atcccggcat aacaaaatcg gaatcgaggc ggatatgatc ctggtctgca
      841 ataaaattga tctgatcgat tcctacatcc acttcttcca ttcctatttc cagttcctga
      901 gtatcgaaag ggtatgctct taagtcggcc tgagtataaa aaagggcatg aaatttccaa
      961 agcttgaatg tcggttcatc tgcaatccgt tctatatgat cgtcatcctg caccatacc
//
LOCUS       DAOQGJ010001990         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132252_length_1019_cov_2.969917, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001990 DAOQGJ010000000
VERSION     DAOQGJ010001990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132252_length_1019_cov_2.969917"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK683_23130"
     CDS             complement(<1..344)
                     /locus_tag="PK683_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11413.1"
                     /translation="MASREFLDRLPEELKQWIKEEFRGLSPQKDALYNAAEAVFGKEN
                     SEFFIELLENENFKFFLKLRRTVSPMRIEGQFERLFDRVMMEIPVPTVAVTAAGMADP
                     DYIKTERYFFSK"
     gene            complement(346..>1019)
                     /locus_tag="PK683_23135"
     CDS             complement(346..>1019)
                     /locus_tag="PK683_23135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11414.1"
                     /translation="YSSLKKAGRLPSEKDFGIELQTRAFYNDKFYVLNANKDKSVWRN
                     FFTGTEENVNPEIIYKVYDRSSIYKKIAAEADRQKLLTELNTQAYSEKEFARVLSRFT
                     ELITSLNETGSSSILKKIGLSDLHEKEDLELLAVHPASTRIESAALKHYLYLLDRGMG
                     RYLAQSYAVRLVIDQICYGQYRDKKLRDNTAYSLINLIARSFRLFADNAKYNTRLITE
                     NLFQN"
BASE COUNT          249 a          230 c          197 g          343 t
ORIGIN      
        1 ttcttggaaa aaaaatacct ttccgttttt atatagtcag gatctgccat tcctgctgcc
       61 gtcactgcga ccgtcggaac agggatttcc atcatcactc tgtcgaacag tctctcgaac
      121 tgtccttcaa ttctcatcgg agaaactgtt cttctcagtt tcagaaagaa cttgaaattc
      181 tcattttcca gaagctcaat gaagaattca gaattttcct ttccgaacac tgcttcggcc
      241 gcattgtaca gagcatcctt ctgcggactt aagcctctga attcctcctt gatccactgt
      301 ttcagctctt ccggaagcct atctaaaaat tccctggatg ccatttcagt tctggaaaag
      361 gttttccgta atcagtcttg tattgtattt tgcattgtct gcaaacagcc gaaatgatct
      421 ggcaatcaga ttgatcagag aatacgctgt attgtctctc agttttttgt ccctgtactg
      481 tccgtagcag atctgatcga tgacaagcct gactgcatag gactgtgcaa gatatctgcc
      541 cattcctctg tccagaagat acagatagtg cttgagtgca gcagattcaa ttcttgtcga
      601 agccggatga actgccagaa gttccagatc ttctttttca tgcagatccg agagtccgat
      661 ttttttcaga atggaggagc ttcctgtttc gttcagcgaa gtgatcagtt cggtaaatct
      721 ggacagaact cttgcaaatt ctttttctga ataagcctga gtgttcagtt cagtcagcag
      781 tttctgtctg tccgcctcag ccgcaatttt tttatatatg ctggatctgt catatacctt
      841 gtagattatt tcagggttca cattttcttc tgttcctgtg aagaaatttc tccagacaga
      901 tttgtcctta tttgcattaa gaacataaaa tttatcatta taaaatgctc ttgtctgaag
      961 ttctattccg aagtcttttt cggaaggaag cctgcctgcc tttttcaagg agctgtatg
//
LOCUS       DAOQGJ010001991         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132290_length_1019_cov_2.518672, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001991 DAOQGJ010000000
VERSION     DAOQGJ010001991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132290_length_1019_cov_2.518672"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1019)
                     /locus_tag="PK683_23140"
     CDS             complement(<1..>1019)
                     /locus_tag="PK683_23140"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNH11415.1"
                     /translation="LGMLFGILYGLIAVYFQWGKFTVDWIKPVGTIFINLLKVVAIPL
                     VFISLVAGITSLRDTSKVKRIGLRTIIIYLFTTVVAVCLGLSLVNITKPGHAFPESKR
                     KELQESFKKEADKKRDAAKDVQDSPPLQALVDMFPVNIFKAASENTQMLQVIVFAFLF
                     GIAMVMIPGDRANLVRPFFEGMNDIILKMIDIIMQLCPYGVFALLASLLVEFAGGDIT
                     KTWDLFQALGLYSLTVLVGLFIMVYVNYGLLLKILTKRSPGNFFRSIFPVQMLAFSTS
                     SSSATLPLTMEHCEKELGVSNEVASFVLPVGATVNMDGTSLYQAVAAVFIAQAMGMNL
                     DFAAQ"
BASE COUNT          290 a          244 c          219 g          266 t
ORIGIN      
        1 gctgagctgc gaagtccaga ttcattccca ttgcctgtgc tatgaatact gcagccactg
       61 cctgatacag acttgttccg tccatattta cggttgcacc cactggaagc acgaaacttg
      121 caacctcgtt ggaaaccccg agttcctttt cgcagtgctc cattgtaaga ggcagtgtgg
      181 ctgaactgga acttgtggaa aaagcaagca tctgcacagg gaaaatggat cggaagaaat
      241 tgcccggact tcttttggtc aggattttca gaagaagacc gtagtttaca tataccataa
      301 tgaaaagacc gacaaggaca gtcagcgaat acagtccgag tgcctgaaac agatcccagg
      361 tcttcgtaat gtctcctccc gcgaattcca ccagaagcga ggcaagaaga gcaaacactc
      421 cgtacggaca tagctgcatg ataatatcaa tcattttcag tataatatca ttcatgccct
      481 caaagaaagg ccggacaaga tttgctctgt ctcccggaat catcaccatt gcaattccga
      541 aaagaaatgc aaatactatc acctgcagca tctgagtgtt ttcggaagct gctttgaata
      601 tgttcactgg aaacatatcc acaagtgcct gaaggggagg gctgtcctga acatctttag
      661 ctgcatctct tttcttgtcg gcttccttct tgaagctctc ctggagttcc tttctcttgg
      721 attccggaaa tgcatgacct ggcttggtga tgtttactaa actgagtcca aggcatacag
      781 ctacaacggt cgtaaaaaga tatataataa ttgttctaag accgattctt tttaccttgc
      841 ttgtatcccg aagacttgtg attcccgcca ccagagaaat aaatacaaga ggaattgcga
      901 ctaccttcag caggttgatg aatatcgttc cgacgggctt aatccagtct acagtgaatt
      961 ttccccactg gaagtatact gcaatcagtc catacaggat gccgaaaagc ataccgagc
//
LOCUS       DAOQGJ010001992         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132347_length_1019_cov_2.054979, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001992 DAOQGJ010000000
VERSION     DAOQGJ010001992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132347_length_1019_cov_2.054979"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PK683_23145"
     CDS             <1..728
                     /locus_tag="PK683_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11416.1"
                     /translation="NERTEELHDKISTVTSIDIKLVEMSRMQEEMDSKLTEVKSLAGK
                     MNEITQAVHNTGRMGSDLNEKLMQLHKELSKLESKEDELQNQINSVEVKASELAARNY
                     DFKSIESKFDKVENLMMDLSTKHKQISTMQKRVDSLKTETEEMRMGLEEILEHAEEKF
                     QKFSEILNQAETVTELSSAKSSRSSKSASVAGREANQDLIKRRRATVLSLYRTYDWST
                     ETIAEKLNMEKSLVEAIVKNQAS"
     gene            833..>1019
                     /locus_tag="PK683_23150"
     CDS             833..>1019
                     /locus_tag="PK683_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11417.1"
                     /translation="MKKLFSFFSVCLFLLSCSMFQKDDKNKDKNKFLLLAAAVASSAN
                     SLSSANCGNVSYSGIACI"
BASE COUNT          352 a          180 c          231 g          256 t
ORIGIN      
        1 ttaatgaaag aacggaagaa cttcatgata aaatcagcac agtaacttcc attgacatca
       61 agcttgtgga gatgtccaga atgcaggagg agatggattc caagctgacc gaagtcaagt
      121 ctcttgcagg aaagatgaat gagattactc aggcagttca taataccgga agaatgggaa
      181 gtgatcttaa tgaaaaatta atgcagcttc acaaggaact ttccaaactg gaatccaagg
      241 aagatgaact tcagaatcag atcaattccg ttgaagtgaa ggcatctgag cttgcagcaa
      301 ggaattatga tttcaagtct atcgagtcta agtttgacaa agtggaaaat ctgatgatgg
      361 atctctccac caaacacaag cagatttcca caatgcagaa aagggtggat tcactgaaga
      421 ctgagacaga ggaaatgagg atgggtctgg aagaaattct cgaacatgct gaggagaaat
      481 ttcagaagtt ttcagaaatt ctgaatcagg cggaaactgt aacagaactg tcatctgcga
      541 agtcctcgag atcttctaaa tctgcatcag ttgcaggcag agaggcaaat caggatctca
      601 tcaagagaag aagagctacc gtcttaagtc tgtacagaac atatgactgg tcgaccgaga
      661 caattgcaga gaaactgaat atggaaaaat ctctggtgga agcgatagta aaaaatcagg
      721 cgagctgaaa aacttttcct tccctgaatg agtggcagtc caataatgaa aaagatcact
      781 caattgagag aaattcgatt gagtgatctg ttttaattaa aagaggtaat ctatgaagaa
      841 actattttct tttttcagtg tatgtctgtt tctcctgagc tgttctatgt tccagaagga
      901 tgataagaac aaagacaaga ataaattcct tcttcttgca gcagctgtgg caagttctgc
      961 aaattctctg tcatcagcaa actgcggaaa tgtttcctac agcggaatcg cctgcatac
//
LOCUS       DAOQGJ010001993         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132390_length_1019_cov_1.780083, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001993 DAOQGJ010000000
VERSION     DAOQGJ010001993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132390_length_1019_cov_1.780083"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /gene="rpsB"
                     /locus_tag="PK683_23155"
     CDS             <1..812
                     /gene="rpsB"
                     /locus_tag="PK683_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNH11418.1"
                     /translation="GHQTRKWNPKMSPYVFTARNGIHIIDLQKTVQKAKEAYDALKKL
                     TGQGKKVLFVGTKKQARGAIEREAKRCNMYYVSHRWLGGLLTNWNTVKKSIARLKRLE
                     SMMENGTFSQEAGTKREQLGLERELEKLRKTLGGIKDMNGIPDILFVIDPKKEEIAVK
                     EAKALGLTVFAVIDTNCDPELIDYAIPGNDDAIRAITLFLETMANAIIEGTGGEVIQP
                     KFSDDFDEETMQSMNYKGEYDEDGKFIMDDDATYTKEFVAAAAVEPPKAGE"
     gene            815..>1019
                     /gene="tsf"
                     /locus_tag="PK683_23160"
     CDS             815..>1019
                     /gene="tsf"
                     /locus_tag="PK683_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007636.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HNH11419.1"
                     /translation="MEISSELIKDLRERTGAGLLDCKKALIENGGNMDKAMDFLREKG
                     LAKAAKRGDRETKEGRVFSYIHAN"
BASE COUNT          369 a          186 c          242 g          222 t
ORIGIN      
        1 tcggtcacca gaccagaaaa tggaatccaa aaatgtctcc gtatgtattc accgcaagaa
       61 acgggataca tatcattgat ctgcagaaga ctgttcagaa agcaaaagaa gcatatgatg
      121 ctctgaaaaa gctcactggc caaggcaaaa aggttctttt tgtaggaacc aagaaacagg
      181 cgagaggtgc gattgaaaga gaagcaaaaa gatgcaatat gtattatgtt tctcacagat
      241 ggctcggcgg acttcttaca aactggaata cagtaaagaa gagtattgcc agactgaaac
      301 gtctcgaatc aatgatggaa aacggaactt tctctcagga agcaggaaca aaaagagaac
      361 agctgggtct cgaaagagaa ctggaaaaac tccgcaagac tcttggcgga atcaaggata
      421 tgaacggaat tccggatatt ctttttgtaa ttgatccaaa aaaagaagaa attgcagtaa
      481 aagaagcaaa ggcacttggt ctcactgtat ttgcagtgat tgatacaaac tgtgatccag
      541 aactgatcga ctatgctatt ccaggaaacg atgatgccat cagagcaatc actctgttcc
      601 ttgaaacaat ggcaaatgcg attattgaag gaactggagg agaagtaatc cagcctaagt
      661 tcagcgatga tttcgatgaa gagacaatgc agagcatgaa ttacaaaggt gagtacgatg
      721 aagacggtaa attcattatg gatgatgatg ccacttacac aaaagagttt gttgccgcag
      781 ctgcagtaga accaccaaaa gcaggagaat aatattggaa atttcatccg aactcattaa
      841 agatcttcgt gaaagaacag gtgcaggact tctggactgc aaaaaagccc tcattgaaaa
      901 tgggggcaat atggacaagg caatggactt ccttcgcgag aagggacttg caaaagccgc
      961 taaacgcgga gacagagaaa caaaagaggg acgtgttttt tcctatatcc acgcgaatg
//
LOCUS       DAOQGJ010001994         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132465_length_1018_cov_4.131880, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001994 DAOQGJ010000000
VERSION     DAOQGJ010001994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132465_length_1018_cov_4.131880"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..410)
                     /locus_tag="PK683_23165"
     CDS             complement(15..410)
                     /locus_tag="PK683_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNH11420.1"
                     /translation="MSLSFPVSPEKNASLKERMEKLGIKESDLEESFIRSGGNGGQNV
                     NKVATAVFLSHKLSGIEVKCSIHRTQGLNRYKARDILCRKLEEKLSGKESGSARHKSK
                     IKKNKIKREKRRREKISEIKAAELESEAE"
     gene            complement(407..796)
                     /locus_tag="PK683_23170"
     CDS             complement(407..796)
                     /locus_tag="PK683_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNH11421.1"
                     /translation="MSREGMELVTQHLVMAASLNSHGNLFGGIMLAWLDEAAAIFGMR
                     KIGYTNIVTVNMDDVDFKTPGRNGDMIQIYACIEKQGRSSFTVRTTAMSLQRDTLQER
                     EVISCRVTFVCLNQEGKPFPFFESRTV"
     gene            complement(793..>1018)
                     /locus_tag="PK683_23175"
     CDS             complement(793..>1018)
                     /locus_tag="PK683_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004433375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNH11422.1"
                     /translation="EITELVRQSAAYAHFVLGCSGYSRTDFIIENKIPYILETNTLPG
                     MTETSLLPQQAAYEGYSMKQILTWILEVLV"
BASE COUNT          242 a          253 c          193 g          330 t
ORIGIN      
        1 cgccttaagc agaattactc tgcctctgat tccagttctg cagcttttat ttctgaaatt
       61 ttttctctgc gtcttttttc tcttttgatc ttgttctttt taattttact cttatgtctt
      121 gcagacccgg attcctttcc ggacagtttc tcctcaagct tcctgcacag aatgtctcta
      181 gccttgtatc tgttgagacc ctgggttctg tggatcgagc atttaacttc aattcctgaa
      241 agcttatgac tgagaaagac agcagtggct accttattga cattctgccc gccgtttcct
      301 ccggatcgga taaaactttc ttccagatcg gattccttga ttccgagctt ttccatccgt
      361 tccttgagac ttgcattttt ttcaggactg actggaaagg aaagactcat acagttctgc
      421 tctcaaaaaa aggaaacggt ttcccttcct gattcagaca tacaaaagtg actctgcagc
      481 tgatcacttc tctttcctga agagtgtctc tctgaagcga catagccgtt gttctgacag
      541 taaaggaaga cctcccctgc ttttcgatgc aggcatagat ctgaatcatg tctccgttcc
      601 ttccaggtgt cttgaaatcc acatcatcca tattgactgt tacaatattt gtatagccta
      661 tttttctcat tccgaatatc gctgccgcct catccagcca ggccagcatt attccgccga
      721 acagatttcc atgagaattt aatgatgctg ccattacgag atgctgagta acaagttcca
      781 ttccctcccg gctcatacaa gaacctcaag tatccaggtc agtatctgct tcattgaata
      841 accttcatac gctgcctgct ggggaagcag gctggtttca gtcattccgg gaagagtgtt
      901 tgtttccagg atatacggta ttttattttc aatgataaaa tccgttctgg aataaccgga
      961 acagccgaga acaaaatgag cataagctgc cgactgtctg accagttcag tgatctct
//
LOCUS       DAOQGJ010001995         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132523_length_1018_cov_2.778816, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001995 DAOQGJ010000000
VERSION     DAOQGJ010001995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132523_length_1018_cov_2.778816"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PK683_23180"
     CDS             <1..808
                     /locus_tag="PK683_23180"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH11423.1"
                     /translation="SIVDIQLGDHIKREMAVLFSDIRSFTSISEHMTPKENFDFVNSY
                     LAKVSPMIRKYEGFIVKYLGDGIMAVFPGNPENAVRSAVEKQFQVDELNRELIQKGQN
                     PINVGIGIHFGPMMVGMVGEMHRIQGDAFSDHVNLTSRLEGLTKYYGTRIIISEDMKN
                     QIRNDLFSIRYLDSVKVVGRKSYIKIYEVMDSDPADLRERKKSCLTMYENALQLYQEK
                     ELESAFDLFQKIKKVLPEDNVLNLYIDRIEKYRKDGLPPDWIAQSEFDSK"
     gene            819..>1018
                     /locus_tag="PK683_23185"
     CDS             819..>1018
                     /locus_tag="PK683_23185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH11424.1"
                     /translation="MAKETEWSKFKSLNRSVAAREVLVQQGYPPTAMYILMDGVVDIF
                     RNDKLISTIRNRGEYIGEISILL"
BASE COUNT          332 a          182 c          244 g          260 t
ORIGIN      
        1 atcgattgtg gacattcagc tgggtgacca tatcaagaga gaaatggcag ttctcttttc
       61 agatatccgt tcgtttactt ccatctctga acatatgact ccaaaggaaa attttgattt
      121 tgtgaattct tatcttgcaa aggtcagccc gatgatcaga aaatatgaag ggttcatcgt
      181 caaatatctt ggagacggga tcatggcagt gttcccggga aatcctgaaa atgcagtccg
      241 ttctgccgtg gaaaaacagt ttcaggtgga tgaactgaac agggaactga ttcagaaggg
      301 gcagaatccg atcaatgtgg gcataggcat ccatttcgga ccaatgatgg taggaatggt
      361 aggagaaatg cacaggattc agggagatgc tttttccgat catgtgaatc tgacatccag
      421 actggaaggg ctgacaaagt attatggaac acgcattatt atcagtgagg atatgaaaaa
      481 tcagatcagg aatgatcttt tttccatccg gtatctggat tcagtaaaag ttgtaggcag
      541 gaagtcatat ataaagattt atgaagtgat ggattccgat cccgcagacc tgagagaaag
      601 aaagaaaagc tgtctgacta tgtatgaaaa tgcccttcag ctctaccagg aaaaggaact
      661 tgaatctgcg ttcgatctgt ttcagaagat caaaaaagtc ttgccggaag acaatgtatt
      721 aaatctgtat attgacagaa ttgaaaaata tcggaaggac ggccttccgc ccgactggat
      781 tgcacagtcg gaatttgaca gcaagtaaga ggcacagtgt ggcaaaagaa acggaatggt
      841 ctaaattcaa atccttaaac agatcagtag cagcaagaga agtacttgtt cagcagggat
      901 atcctccgac tgcaatgtac atactgatgg acggtgtagt agatatcttc agaaacgaca
      961 aactcatttc tacgatcaga aacaggggcg aatacatagg agaaatttcc attctact
//
LOCUS       DAOQGJ010001996         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132785_length_1017_cov_2.673597, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001996 DAOQGJ010000000
VERSION     DAOQGJ010001996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132785_length_1017_cov_2.673597"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PK683_23190"
     CDS             complement(<1..468)
                     /locus_tag="PK683_23190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11425.1"
                     /translation="MKLNDDHEKLQTSGLEENAEMSSENEQKLFAAEESIWFAIKGFQ
                     EKSLFSDFIGDSAVELDIAKAEKLMAMEPKYKVPEYLKNYVNMNLNSSSEKSESVVIR
                     IVKEGVRFINSAVESVFKAETVFLPAMRSPEKQDLQAVNLYADADGTQITYQLI"
     gene            complement(509..>1017)
                     /locus_tag="PK683_23195"
     CDS             complement(509..>1017)
                     /locus_tag="PK683_23195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH11426.1"
                     /translation="TEGFYRILSDIKIENRVIFKLAYIYYLNLEEDEIKYIQEKNNYS
                     KEYILSKIMEIRDFLSDKELESIELEDKITSLYNSILNLREIQKKQGYKEFEDNLPQI
                     DKIQQNIDKKYEQRRKLLERRHKGHFITKTPHKMITSFLQIPEGGVSISIQRVLEKIQ
                     KKISSNEI"
BASE COUNT          272 a          209 c          173 g          363 t
ORIGIN      
        1 gatcagctga tatgtaatct gagttccgtc ggcatccgca taaagattga ctgcctggag
       61 gtcctgcttt tccggtgacc gcattgcagg aagaaagact gtttcagcct taaacactga
      121 ctcaacggct gagttgatga atctgactcc ctcttttacg attcgaatta ctacagactc
      181 agatttttct gaactggagt tcagattcat atttacatag tttttgaggt attcagggac
      241 tttgtatttg ggttccattg ccatgagttt ttcggccttc gcaatatcga gttcgaccgc
      301 agaatctcca ataaaatcac tgaacagaga tttttcctga aaacctttta tggcgaacca
      361 gatagattct tctgccgcaa aaagcttttg ttcattttca gaactcattt ctgcattctc
      421 ttccaagccg gaagtttgga gtttttcatg gtcatcgttc agtttcataa gatcctcacg
      481 ctacatatta agacagaaaa gtttagaatt atatttcatt gctcgaaatt tttttttgaa
      541 ttttttccaa aactctctga attgaaatac tcacaccgcc ttccggaatc tgcaaaaaag
      601 aagtaatcat cttgtgcggt gtctttgtaa taaaatgacc tttatggcgt ctttccagaa
      661 gcttccgtct ctgttcatac tttttatcaa tattctgctg aatcttgtca atctgaggga
      721 gattgtcctc aaactccttg tacccctgtt ttttctggat ttctctcaaa ttcagtatgc
      781 tgttgtacaa ggatgtaatc ttatcttcca gctcaatgga ttccagttcc ttgtccgata
      841 gaaaatcacg gatttccata atcttgctta aaatatactc tttgctgtaa ttattttttt
      901 cctggatata cttaatttcg tcctcttcca gattcagata ataaatatag gcaagtttaa
      961 aaatgactct attttcaatt ttaatgtcag aaagtatgcg gtaaaaccct tcagtct
//
LOCUS       DAOQGJ010001997         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_132812_length_1017_cov_2.435551, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001997 DAOQGJ010000000
VERSION     DAOQGJ010001997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132812_length_1017_cov_2.435551"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PK683_23200"
     CDS             <1..300
                     /locus_tag="PK683_23200"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HNH11427.1"
                     /translation="KVDSPYTGLAVLFAEHLDYNLVFNPLEDNNVVRGNLADDLSDIY
                     LDLKRPLLTFSRGGEKNQENAYWQWKFNISSHSGNHILNSLKTIHWINYHYGTQN"
     gene            824..>1017
                     /locus_tag="PK683_23205"
     CDS             824..>1017
                     /locus_tag="PK683_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11428.1"
                     /translation="MNSDKVLFTANRFCILNTVFIIYSFLFLFTGCLKVKRSPFDVSS
                     PSGSILTNAIIAAFAKIVPPS"
BASE COUNT          307 a          197 c          196 g          317 t
ORIGIN      
        1 aaagtggatt caccgtacac aggtcttgca gtcctttttg ctgaacatct ggattataat
       61 cttgttttta atccattgga agataataat gtggttagag gaaatttagc tgatgattta
      121 tctgatatct acctggattt aaaaagacct cttttgactt tttcacgggg tggtgaaaag
      181 aatcaggaaa atgcatattg gcagtggaaa ttcaatatca gttctcacag cgggaatcat
      241 atactgaatt ctttaaagac aattcattgg ataaattatc attatggcac tcagaactga
      301 ctgcagaatg gagtaccatc tgttctctgc tcttccgaaa actgtcatcg attcacgaat
      361 atgaaaaaga tacactttga gtttttccgc acttcctgca ggaagggact gcacatcact
      421 caatgtatga atgtctgaat aactcacctt acgccttcag ctgcaggcgg ggcgttttgg
      481 gcaggaacgc ctgccgcggc ttgatggctc gcacacgtgg aagctgctgc acacgtggaa
      541 gctgctgcat aagtgaaacc cgaaataagc aatgcttttc gttcagaaaa atgataaata
      601 aattgataac agaaagacga ctgaagtttt tttaagctgc tctcgaataa agcataaaag
      661 cagtatattt ttcttccttt ctacaaaata atcagtccaa aatatattcc gaatatttcg
      721 gtatggaaaa atgcttgatt ctgctgtttg aactctcaga atgtgaaaat atttcaccga
      781 gaatcaggct tgaaactttt ttctggcaag agaaggaaat gaaatgaatt ctgataaagt
      841 cttattcacc gcaaaccggt tctgcatact taatacagta tttataattt attccttttt
      901 atttttattc accggatgtc tgaaggtaaa gaggtctcca ttcgatgtat catccccttc
      961 cggttccatt ctgacaaatg caatcattgc cgcttttgcc aagatagtcc ctccttc
//
LOCUS       DAOQGJ010001998         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_133203_length_1015_cov_5.912500, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001998 DAOQGJ010000000
VERSION     DAOQGJ010001998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133203_length_1015_cov_5.912500"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PK683_23210"
     CDS             complement(<1..964)
                     /locus_tag="PK683_23210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH11429.1"
                     /translation="MGTIQDITVLKQAELLLKDAKEKAEAASRAKSEFVANMSHEIRA
                     PLNGVIGFNELLSLTPLNPIQKQYTENINTSAVSLLGIINDILDFSKIEAGRLELDEI
                     RISIRELLEESCDIVKLSAGRKKIEIILNIPPDVPEYMTADPVRLRQVMVNLLGNAVK
                     FTERGEIEISLKFQELSEKNQGIFHFSVSDTGIGITEEQKERLFQSFVQADSSIGRKF
                     GGTGLGLSISKGLISEMGGNLELESSLGAGSRFYFSLKRGYERSEPPDAFIGFRKVLV
                     CVQNVRTAEMICRMLDRINVFSEVEYSAHSAVSRLSLDRNFDCII"
BASE COUNT          271 a          242 c          196 g          306 t
ORIGIN      
        1 gaataatgca gtcaaaattt ctgtccaagg aaagcctgga tactgcggaa tgtgcagaat
       61 attcaacctc ggagaataca ttgattctat caagcattct gcagatcatc tctgcagttc
      121 tgacattctg aacacagaca agcacttttc gaaaaccgat aaatgcgtca ggcggctcag
      181 atctttcata tcctcttttc agagaaaaat aaaacctgct ccccgctcca agactgcttt
      241 caagttcaag atttcctccc atttctgaaa tgagaccctt tgaaatggaa agtccaagcc
      301 cggttcctcc gaattttctt ccgatcgatg aatcagcctg aacaaaggac tgaaaaagcc
      361 tctctttctg ttcttccgta attcctatcc cagtatcaga tacagaaaaa tgaaatattc
      421 cctgattctt ttcggaaagt tcctgaaact taagactgat ttcaatctct cctctttccg
      481 taaatttcac tgcatttccg agaagattca ccatcacctg ccgaagtcgc actggatcag
      541 ccgtcatata ttcaggaacg tcaggcggaa tgttcagtat gatttcaatt ttctttcttc
      601 ctgcagaaag ttttacgata tcacaggatt cttcgagaag ctcgcggatg gagattctga
      661 tttcatccag ttccagcctg cctgcttcaa ttttggaaaa atcaagaata tcatttatga
      721 ttccgagaag ggagactgca gaagtgttta tgttttcagt gtactgtttc tgaatcgggt
      781 tcagaggagt cagagagaga agctcattaa aaccgatcac tccgttcaga ggtgccctga
      841 tctcgtgact catgtttgca acgaattcgc ttttggccct gctggctgcc tctgcctttt
      901 ccttggcgtc cttgagcaga agttctgcct gcttcagaac agtaatgtcc tgaatagtgc
      961 ccactgatct gagaggtttt cctgattcgt caaaatttgt actgcattcc tccct
//
LOCUS       DAOQGJ010001999         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_133272_length_1015_cov_2.787500, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010001999 DAOQGJ010000000
VERSION     DAOQGJ010001999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133272_length_1015_cov_2.787500"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1015)
                     /locus_tag="PK683_23215"
     CDS             complement(<1..>1015)
                     /locus_tag="PK683_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH11430.1"
                     /translation="VDFSDDKNKPKNYLKGLIRTILMYAKDLSSNNPMFTEDMKMTLV
                     NISEPGKIADFVGSVLNLEKNEYQEIIESLEIHDRLEKVLLFTRKELELLDVQRKIND
                     QVNQKMDRNQRQYFLREQLKAIQGELGLGEDKSEKKFTSLEERLIKARVPEEVLAEVK
                     RELEKLNFIEPHSAEYQVLRNYLDMIEALPWEEPPVRNVNLKKSRQILEEEHYKLEEV
                     KKRILEFLAVRKLKNDSRSPVIICLVGPPGVGKTSIAKSIAKSLDRKFHRISLGGMRD
                     EAEIKGHRRTYIGALPGKILSALRITKEKDSVVLLDEIDKLKSGFTGDPAGALLEVLD
                     PEQN"
BASE COUNT          243 a          242 c          189 g          341 t
ORIGIN      
        1 attctgctca ggatcaagaa cctcaaggag ggctcctgca ggatctccgg taaatccgct
       61 cttcagtttg tcgatctcat cgagaagcac tacagaatct ttttcttttg taatcctgag
      121 cgcagaaagt atcttgcctg gaagcgcccc tatgtatgtt cttctgtgtc ctttgatttc
      181 tgcttcatct ctcataccgc cgagagagat tctgtgaaac tttctgtcga gcgactttgc
      241 aatggatttt gcaatggagg ttttccccac gccgggagga ccgacaagac agataattac
      301 agggcttctg gaatcatttt tgagttttct cactgccaga aactcaagta ttcttttttt
      361 cacctcttcc agtttataat gctcttcctc cagaatctgt ctggactttt tcagattgac
      421 gttcctgaca ggcggttctt cccacggaag agcctctatc atatccagat agtttctgag
      481 aacctggtat tctgcagaat gaggctcaat gaaattcagc ttctccagtt ctcttttaac
      541 ctctgcaagc acttcttccg gtacacgggc ctttataagc ctttcttcca aagaggtgaa
      601 ttttttttca gatttgtctt cacctagtcc aagctctccc tgaattgcct tcagctgttc
      661 gcgaagaaag tactgacgct gattcctgtc catcttctgg ttcacctggt cattgatttt
      721 tctctgaaca tccagaagtt ccaattcctt tcttgtgaat aggagaactt tttccagcct
      781 gtcatgaatt tccagagatt caataatctc ctgatactca tttttttcga gattcagaac
      841 agacccgacg aaatcggcaa tttttccggg ctcactgatg tttaccagag tcattttcat
      901 atcctcagta aacattggat tattcgagga aagatctttc gcatacatta aaattgtacg
      961 gatcagtcct ttcagatagt ttttaggctt attcttatca tcagaaaagt ctaca
//
LOCUS       DAOQGJ010002000         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_133291_length_1015_cov_2.662500, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002000 DAOQGJ010000000
VERSION     DAOQGJ010002000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133291_length_1015_cov_2.662500"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1015)
                     /locus_tag="PK683_23220"
                     /pseudo
     CDS             complement(28..1015)
                     /locus_tag="PK683_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT          259 a          274 c          228 g          254 t
ORIGIN      
        1 ctgttacagg cttgatatta attaatgact gtggtgaaat accggcttct tgaaaatata
       61 agacacagca gcaggaaata cggtcatact gaaaagaagg gctgctgcaa gagccagagc
      121 cacagtgact gccatgggac ggaacattct gccctcgaca ccttcgagcg ccatcagtgg
      181 gagatataca agcatgatga tcgccacagc aaacgccgca gcgcgcccga ctttcttgca
      241 gctctggagt acgatcattt ttgtcagatc ttccctttct tcctctgact gagacctgtc
      301 atagagcgcc cggctggtaa aaaaaccggc aagcactgct tcgagcatta cgatggatcc
      361 gtccacaaga agaccgaaat caagagctcc gagactcatc agattcccga caactcccag
      421 ctgtctcata aatatgactg ccacaagcat ggatacagga attgcaagag ccacaagcgc
      481 accgcctttc actgttccga gagtcacgat aagtgctata aaaacgagaa gagcaccttc
      541 cgcaagattg atgaatacgg tcttcagcgc gcggttgatg aattccgacc tgtcataaaa
      601 aggttcaatc ttcattccct gcgggaggtt ctttcgaagc tcctgcacct tttccttgac
      661 ctttgccacc acctctcttg aattctgtcc gatgagcatc atgactgttc cgccgacgac
      721 ttctttttca tcccttgtca tcagtccgaa acggatggac gggccgatct tgacatttcc
      781 cacctgcccg agaagaagag gcaccccgtc agaatttgtg cggatggcag tctgagcaag
      841 ttcatcaatt gtcttgaact gaccctctcc cctgatgaca agctgttcgt tgcccttcat
      901 aatgtatccg ccgcctacat tggcgtttgc tcttttcagt tcattcagta tgctcgaaag
      961 agtcaggttg tgtgaagcaa gtcttctcgg atcaatgatc acctggtact gcttg
//
LOCUS       DAOQGJ010002001         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_133543_length_1014_cov_2.611053, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002001 DAOQGJ010000000
VERSION     DAOQGJ010002001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133543_length_1014_cov_2.611053"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..194
                     /locus_tag="PK683_23225"
     CDS             57..194
                     /locus_tag="PK683_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11431.1"
                     /translation="MTKIDVFRKFIVMYKEIESYHRLPFPDEGKYALNAQKKTIDLIA
                     E"
     gene            205..534
                     /locus_tag="PK683_23230"
     CDS             205..534
                     /locus_tag="PK683_23230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11432.1"
                     /translation="MKYLMISILSSEKTVHIKKEIWTASCHPEQQFPSWRVEGCLSGK
                     SWFEALTMTGFEATAGLLLQIEHLFNSFKHFVNTHIVASPTFLYAGHFSLNCILSYNS
                     FYGRQND"
     gene            620..>1014
                     /locus_tag="PK683_23235"
     CDS             620..>1014
                     /locus_tag="PK683_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11433.1"
                     /translation="MDGSGANLALFFSERNLEVVEFKADILMPKTSKITDEENKWYND
                     YGVNKYSLNTAWKYYRIDEPIQLGTYKFNSNQTYNFPLPVGKYTLLLQYKSDAELNLN
                     KTVFLKELGKYSVKNRVGSKNELKFEEIE"
BASE COUNT          351 a          169 c          184 g          310 t
ORIGIN      
        1 tatcctttga cgaactgacc tcttaacagg caataaatca aatttaatag aacagattga
       61 caaaaataga tgtatttcgt aaattcattg ttatgtacaa agaaattgaa tcttatcaca
      121 ggcttccgtt tcctgatgaa gggaaatatg cactcaatgc acagaaaaaa acaatagatc
      181 tgatcgcaga atgaggagaa aaatatgaaa tatttaatga taagtatact cagttcagag
      241 aaaactgtac atataaagaa agaaatatgg acggcatcct gtcatcctga gcagcagttt
      301 ccaagttggc gtgtcgaagg atgcctttct ggaaagtcat ggtttgaagc gctcactatg
      361 acaggctttg aagccactgc aggtctgctt ctgcaaatcg aacatctgtt caattccttc
      421 aagcattttg taaatacaca tatagttgca agtccaactt tcctttatgc gggacatttc
      481 agcctgaact gtatcctcag ttataattca ttttatggaa ggcaaaatga ttaaattcaa
      541 gagtttattt ttactcctga tatgtataac taattgtact gtgattcggt atagattctt
      601 ttcctattat ccttttcgct tggatggaag cggtgctaat cttgcacttt tcttctctga
      661 gcggaatctt gaagttgttg aatttaaagc agacattctt atgccgaaaa cttcaaagat
      721 tacagacgaa gagaataagt ggtacaatga ttatggtgta aataaatatt cattaaatac
      781 tgcgtggaaa tattatagaa tagatgagcc aattcaacta ggcacataca aattcaattc
      841 aaatcagacc tataatttcc ccctccctgt cgggaaatat actctcttac tgcagtacaa
      901 atcagatgct gagctgaatt tgaataaaac cgtatttcta aaagaattgg gtaaatactc
      961 agtaaaaaat cgggtgggtt caaaaaatga attaaaattt gaggaaatag aatg
//
LOCUS       DAOQGJ010002002         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_133699_length_1014_cov_1.364964, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002002 DAOQGJ010000000
VERSION     DAOQGJ010002002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133699_length_1014_cov_1.364964"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..>1014
                     /locus_tag="PK683_23240"
     CDS             147..>1014
                     /locus_tag="PK683_23240"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH11434.1"
                     /translation="MKRSALFLMIITLNCGPLLLKKKPNHPSDRKNTSLLGMFFNRPS
                     SLAVSSADTFDSTPPSVIGTVPFSGGTEVALDSTIQVNFSEPILSSSVNSLRFYVKEN
                     GSVLEGTIKVQSDKIYFTPKLQLLSNRVYSVTVGSEIEDGSGNRMGSDYSFQFTSARM
                     DSVPPSVVTVSPSDGTLGVSQNAVLTVLFSEEIDPSSILKENILAVDEVGTPIQLILS
                     LSGNILTIAPADKFTSGSQHTVTLRSQLRDLSQNTMNGDFIFQFYTADPERPYLVDTQ
                     PKNGDKNFDPRYL"
BASE COUNT          265 a          211 c          231 g          307 t
ORIGIN      
        1 gccaatgatg caggaaggtc acttgctgta gattcagccg ggaatgttta tttgacggga
       61 gagacagtat ccagtttgaa cgggcagtcc ttttttgggg ggactgatat attccttatg
      121 aaatttgaca atgcgggaaa tgtaaaatga agagatcagc actgtttcta atgataatta
      181 cattaaactg cggtccgctt ctcttaaaga agaagccgaa tcatccttct gaccgtaaga
      241 atacttcctt gcttggtatg ttttttaatc gaccctccag ccttgctgta tcaagtgcgg
      301 atacattcga ctccactcct ccgtcagtca ttggtactgt tccgttttct ggagggacag
      361 aagttgcctt ggacagtacg attcaggtga acttcagtga gccgattttg tcttcttctg
      421 tgaacagtct tcgtttctat gtcaaggaaa acggatcagt tctggaaggg acaatcaagg
      481 tgcagtcaga taaaatttat tttaccccaa agctgcagct cctttcgaac agagtttatt
      541 ctgttacagt tggaagtgaa atagaagacg gttccggcaa ccgtatggga tcggattaca
      601 gttttcagtt tacgtccgca agaatggatt cggttcctcc atcagtagtt acagtgagtc
      661 cttccgacgg aactcttggg gtaagtcaga atgcagttct cacagtcctg ttttccgaag
      721 aaattgatcc ttcttccatc ttgaaagaaa acattttagc agtcgatgag gtcggaactc
      781 ccattcagct gattctttca ctctcaggaa atattctgac cattgcacct gcggataaat
      841 ttacttccgg ttctcagcat acggtgactc tccgtagtca gttgcgtgat ctcagtcaga
      901 atacaatgaa cggtgatttt atatttcagt tctatactgc agaccctgaa cgtccgtatc
      961 tggtggatac acagcctaaa aacggggata agaattttga tccgaggtat ttgg
//
LOCUS       DAOQGJ010002003         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_133759_length_1013_cov_3.823591, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002003 DAOQGJ010000000
VERSION     DAOQGJ010002003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133759_length_1013_cov_3.823591"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK683_23245"
     CDS             <1..214
                     /locus_tag="PK683_23245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNH11435.1"
                     /translation="RTFRSIEINKEELEFSEGFTDLHTVSYKEILAGRGYGLSHARES
                     IVIASMIRNAEIRKTDRIHPFTAKLK"
     gene            211..786
                     /locus_tag="PK683_23250"
     CDS             211..786
                     /locus_tag="PK683_23250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNH11436.1"
                     /translation="MSFFIHETSICDEGVLIGNGTKVWHFSHILKGSSIGENCSFGQN
                     CVVGPNVKVGNGVKVQNNISIYEGVEVEDDVFLGPSMVFTNVINPRSFIVRKTEFKKT
                     LLKKGCSVGANSTIVCGVTIGRYALIGAGAVVTKDVPDFGMFYGNPGKLQGWVSIAGN
                     RLQFENGKAMDSFDGTEYEIRDGLCFQISKS"
     gene            794..>1013
                     /locus_tag="PK683_23255"
     CDS             794..>1013
                     /locus_tag="PK683_23255"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNH11437.1"
                     /translation="MKINFNDLKIQYNLLKADLDVSIGKVLEKQNFIMGEEIAALENE
                     LKNFSGAKHCITCANGTDAIQLALRAVGI"
BASE COUNT          315 a          182 c          251 g          265 t
ORIGIN      
        1 gagaaccttc cgctccatag aaatcaacaa ggaagaactg gagttttcgg aaggctttac
       61 agatcttcac acggtgagct acaaggagat ccttgcagga agaggatatg gattatccca
      121 tgccagagaa tcaatcgtca tagcttctat gatcagaaat gctgagatca ggaaaacaga
      181 ccgaattcat ccattcactg cgaaactgaa atgagttttt ttatccatga aacttctatt
      241 tgtgatgagg gagtccttat aggcaatggg acgaaggtgt ggcatttttc ccatattctg
      301 aaaggatcaa gtatcggaga aaactgttcc ttcggtcaga actgcgtagt cgggccgaat
      361 gtaaaagtcg gaaatggagt taaggtgcag aacaatatct ctatctatga aggcgtggaa
      421 gtggaagatg atgtgtttct tggcccttct atggtattca ccaatgtcat caacccgcgc
      481 agtttcattg taaggaagac tgagtttaag aaaactcttc tgaaaaaagg atgcagtgtg
      541 ggagcgaatt ccactattgt atgcggagtt acgataggca ggtatgcatt gatcggtgca
      601 ggagctgttg taaccaaaga tgttcccgac ttcggaatgt tttacggaaa ccctggaaaa
      661 cttcagggct gggtcagcat tgccggaaac agactgcagt tcgaaaacgg aaaggcaatg
      721 gacagttttg acggaactga atatgagatc cgtgacggac tctgttttca gatttccaag
      781 agctgagaaa actatgaaaa taaactttaa tgatttaaaa atacagtata atctgctgaa
      841 agcagatctc gatgtttcca taggaaaagt cctggaaaag cagaatttca ttatgggtga
      901 agagattgct gctcttgaaa atgagctgaa aaatttttcg ggagcaaaac actgcataac
      961 ctgtgcaaac ggaactgatg ctattcagct ggctcttcgt gctgtcggaa tcg
//
LOCUS       DAOQGJ010002004         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_134009_length_1012_cov_3.719958, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002004 DAOQGJ010000000
VERSION     DAOQGJ010002004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134009_length_1012_cov_3.719958"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1012
                     /locus_tag="PK683_23260"
     CDS             <1..>1012
                     /locus_tag="PK683_23260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH11438.1"
                     /translation="LGRLLSAERAFFYVMSENRNAHASNIWFRSNENQAADFSGMFNI
                     NVSEECAAEIEKNGFVLIHEDEIRNKGNICPFYRILDKDTKSLYCVSIFGAMQKMFGF
                     LCFQSLSNIDLNEEEIILLNLLASSVSETVQKAETELDLFRTNNELFQFEYATNQVSI
                     ISRTDKKGRIRFVNENFTKISGYTAGELIGAKHSIVNSGFHPKQFWTEMWKTVTSGRV
                     WREDIRNRTKKGSYYWVDSFIIPMMDINGSITGYLSIRYDITGRKIMEQNLLRAVNDL
                     NETQRQAKLGRWELDLGNDILLWSPGIFDIFEIDPEVFPATYEGFLHFVHPEDRDTVN
                     KAY"
BASE COUNT          309 a          194 c          233 g          276 t
ORIGIN      
        1 ctcggacgtc ttctttccgc tgaaagagcg tttttttatg tgatgtcgga aaacagaaat
       61 gcacatgctt ccaatatctg gttccgttcg aatgaaaacc aggcagccga tttttccggg
      121 atgttcaata taaatgtatc cgaggaatgt gcggctgaaa tcgagaaaaa cggttttgtg
      181 ctgattcacg aggatgagat ccgcaataaa ggaaacatct gtccgtttta caggatactc
      241 gataaagata caaaatcatt atattgtgtt tctatattcg gggccatgca gaaaatgttc
      301 ggctttctct gctttcagtc cctttcgaac attgatctga atgaagaaga gatcatactt
      361 ctgaatcttc tggcatcctc tgtctctgaa acagtgcaga aggcagaaac agagctggat
      421 cttttcagaa caaataatga actttttcag tttgagtatg cgaccaatca ggtgagcata
      481 atttcaagaa ccgataaaaa gggaaggatt cgattcgtaa atgaaaactt cacaaaaatc
      541 agcgggtaca ctgcaggcga actgatcgga gcaaagcaca gcattgtcaa ttcaggattt
      601 catccgaagc agttctggac agaaatgtgg aagactgtca cttccggaag agtctggagg
      661 gaggacattc ggaacagaac aaaaaaggga agctattact gggttgattc gttcattatt
      721 ccgatgatgg acatcaacgg aagtattacc ggctatcttt ccatccgtta tgacattacc
      781 ggcagaaaaa ttatggaaca gaacctgctg agagcggtaa atgatctgaa tgaaacccag
      841 agacaggcca agctcggtcg gtgggaactg gatctgggaa acgacattct tctctggtct
      901 cccggaatat tcgatatttt cgaaattgat cctgaggttt tcccggcaac atatgaaggt
      961 tttcttcatt ttgttcatcc tgaggacaga gatactgtaa ataaagctta tt
//
LOCUS       DAOQGJ010002005         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_134234_length_1011_cov_3.785565, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002005 DAOQGJ010000000
VERSION     DAOQGJ010002005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134234_length_1011_cov_3.785565"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PK683_23265"
     CDS             <1..544
                     /locus_tag="PK683_23265"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11439.1"
                     /translation="IALHGGSGHGADFFWSWLRDARTFGFVLAAPTSIDRTWSLHTPG
                     TDAVNLNRMLSEISSKINIDANHILLTGLSDGGTYSMILSTVRQSPFTHFAPVASAAH
                     VLLNRSGVISVPVKDLPVYLVHGTKDWMFSVQKARETASALEKSGAKLVYREIPDLSH
                     NYPRDENISILKWFYPARFE"
     gene            560..994
                     /locus_tag="PK683_23270"
     CDS             560..994
                     /locus_tag="PK683_23270"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNH11440.1"
                     /translation="MNTKHTLDDYKIGEKFVTPGRTITESDIVSFAGLSGDFYSLHTD
                     SVYAEKTQFGERIAHGMLVLSIGTALPYRLGPNYFLPESFIAFYGMENVRFTAPVKIG
                     DTIRFEGEVTGIDMKDERRGVLTWTGRILNQEDRQEQSQCSH"
BASE COUNT          266 a          225 c          245 g          275 t
ORIGIN      
        1 cattgcactg cacgggggat ccggacacgg ggctgatttt ttctggagct ggcttcggga
       61 tgcaagaaca ttcggttttg tccttgccgc tcctacctca atcgacagaa cctggtcgct
      121 tcatacgcct ggcacagatg cagtgaatct gaacaggatg ctctcggaga tatcctcaaa
      181 aatcaatatc gatgcgaatc atatccttct tacaggtctt tccgacgggg gaacgtattc
      241 catgattctg tccacagtac gtcagtcgcc attcactcat tttgcaccgg ttgcatcagc
      301 ggctcatgtt cttctgaaca ggtcaggggt catttccgtt ccagtaaagg atctgcctgt
      361 ttaccttgtg cacggaacaa aggattggat gttttcagtt cagaaggcgc gtgaaactgc
      421 ttccgcactc gaaaagtcag gagcgaaact cgtttacagg gagatcccgg atctctctca
      481 taattatccg agagatgaaa atatttctat actgaaatgg ttttatccgg cacgttttga
      541 gtaacataaa ggaaatataa tgaacacaaa acatacactc gatgattata aaatcggtga
      601 gaaatttgtc acgcctggcc gtacgattac tgaatccgat atcgtttcct ttgccggtct
      661 gtccggtgat ttctattccc tgcacacgga ttctgtctat gcagaaaaga ctcagttcgg
      721 agagcggatc gcacacggaa tgcttgtgct ttcgatagga acagcactgc cttacaggct
      781 gggtccgaat tattttcttc ctgaatcctt cattgcattt tacggaatgg aaaatgtccg
      841 ttttactgca cctgtgaaaa taggggatac gatccgtttt gaaggagagg tgaccgggat
      901 tgatatgaag gatgaaagga ggggagttct gacctggacg ggccgcattc tgaatcagga
      961 agacagacaa gaacaatctc aatgcagcca ttgaaaatgt gaatgcactt g
//
LOCUS       DAOQGJ010002006         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_134347_length_1011_cov_2.298117, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002006 DAOQGJ010000000
VERSION     DAOQGJ010002006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134347_length_1011_cov_2.298117"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1011
                     /locus_tag="PK683_23275"
     CDS             <1..>1011
                     /locus_tag="PK683_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11441.1"
                     /translation="GKKTDEEKAEEEFPEETFTPDVPDSLVTEDIPTEETPDTFDFGD
                     DLTGKEEGTPETDPFNFDQDFTDENKKDESPVEEPFDFGDEPFGKDETAKTDDTSADP
                     FADINSDNDPFAFDKEDKKDQGDIDPFADGGDDPFSAVSEKTPEADFDPFADMGDTDT
                     TPEEVKSETDIPDFNDIDDGFHSDSSISGTDSTDKWGDIDDDFSSLANIDAGTNKRPE
                     TFPITEEESTDIFNGFENTSEPERTDFTYEPQDSIPNFTDTESEDHGDVDHFADFSPS
                     KATFDEGIDTDESPSLGQLDSEDSYDSGDFTDDDFSGIGEEPAKEHELSDEELAVIQQ
                     EIL"
BASE COUNT          316 a          191 c          228 g          276 t
ORIGIN      
        1 ggaaagaaaa cagacgagga aaaagctgag gaagagtttc ctgaagagac tttcactccg
       61 gatgttcccg acagtctagt cactgaggac attccgactg aggaaacacc ggatactttt
      121 gattttggag atgaccttac tggaaaagag gaaggaactc ctgaaactga tccattcaat
      181 tttgatcagg attttacaga tgaaaataaa aaggatgaat ctccggttga agaacctttt
      241 gactttgggg atgaaccttt cggaaaagac gaaacagcca aaactgacga tacttctgct
      301 gatccgtttg cagatataaa ttccgataat gatccttttg cttttgataa agaagataag
      361 aaagaccagg gagatataga tccttttgcg gacggcgggg atgatccttt ctctgctgtt
      421 tcagaaaaaa cacctgaagc tgattttgat ccatttgccg atatgggtga tacggatacc
      481 actcctgaag aagtcaaatc tgaaactgat attcctgatt ttaatgatat cgatgacggt
      541 ttccattccg attcgagcat ttccggaact gacagcactg ataaatgggg agatattgat
      601 gatgatttct cctctcttgc aaatattgat gctggaacaa ataaaagacc ggaaaccttc
      661 ccgatcaccg aggaagaaag cactgatatt tttaatggct ttgaaaacac ttctgagcct
      721 gaaagaacag actttacata tgaacctcag gattcgattc caaattttac agacactgaa
      781 tccgaagatc atggagatgt agatcatttc gcagatttct ctccaagcaa ggctactttt
      841 gacgagggaa ttgatacaga cgagtctcct tcgcttggac agctggattc ggaagattct
      901 tatgattccg gtgattttac agatgatgat ttctccggga taggagagga acctgccaag
      961 gaacatgagc tttctgatga agaacttgct gtcattcagc aggagattct t
//
LOCUS       DAOQGJ010002007         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_134501_length_1010_cov_3.958115, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002007 DAOQGJ010000000
VERSION     DAOQGJ010002007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134501_length_1010_cov_3.958115"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK683_23280"
     CDS             complement(<1..282)
                     /locus_tag="PK683_23280"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH11442.1"
                     /translation="MLFGIFKIPLLDNIEKENTPQEIREILKGFYSVIKDSDQYIQLV
                     FITDESKFSRISLNDVILNYLSSLLTEKEVNKVKLYTLLESGNVEGMRQL"
     gene            383..>1010
                     /locus_tag="PK683_23285"
     CDS             383..>1010
                     /locus_tag="PK683_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11443.1"
                     /translation="MIGKQISLLLILSFSFLSAEDHSPSVKELQKILKRKFLSDEDAK
                     SVILKSADKKGRYCDYNFHAEHLEVQGSADNAYLMDTKNRESSAFAYLSVCEGMNKNE
                     FIITERCCPMGPCYKSSLFRKEKNRLKFHKKIEGDAYYITDKKGRTFAAVPDNFMSGI
                     MFYGEIKKDGLHPVFASKANEITVPDEFTYPKEKLQLQKIGLLASPSAE"
BASE COUNT          310 a          212 c          199 g          289 t
ORIGIN      
        1 gagctgcctc atcccctcca cattcccgga ttcaagcaga gtgtaaagct tcaccttgtt
       61 cacttctttt tctgtaagca ggctggacag gtaatttaat atgacgtcat tcagactgat
      121 gcggctgaat ttactctcat cggtaatgaa cacaagctga atatactgat cggaatcctt
      181 gataactgaa taaaaccctt tcagaatttc tctgatttcc tgaggtgtat tttctttttc
      241 gatattgtcc agtaaaggaa ttttgaaaat gccgaacagc aagcagtttt attttcggcg
      301 aactgccaga gcgagcgggt ggctctattc tgtgtctttt ccgctttacc gccatatcaa
      361 ttcaaatata atgagtactg ctatgatcgg aaaacaaatc tctcttcttc taatcctttc
      421 tttttcgttt ctctctgcag aagatcactc cccttcagtc aaggagcttc agaaaattct
      481 gaaaagaaaa tttctttcag atgaggatgc gaaatcagtc atactcaaat ctgcggataa
      541 gaaaggacgg tactgcgact ataattttca tgcagaacat ctggaagtgc agggatcggc
      601 tgacaatgcc tatctgatgg acacaaagaa cagggagtca tctgcatttg cgtatctttc
      661 cgtctgtgaa ggaatgaata aaaatgaatt tatcattaca gaaagatgct gtccgatggg
      721 accctgctac aaatcatcac tgttccgaaa agaaaaaaac cgtctgaaat ttcataaaaa
      781 aattgaaggg gatgcatatt acatcactga taaaaaaggc agaacattcg cagctgtgcc
      841 ggataatttc atgtcgggaa tcatgttcta cggagaaata aagaaagacg gacttcatcc
      901 ggtctttgca tccaaggcaa atgaaattac tgttccggat gaattcacat atcccaaaga
      961 aaaactgcag ctgcagaaaa taggcctcct tgcttctcct tcagctgaag
//
LOCUS       DAOQGJ010002008         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_134617_length_1010_cov_2.314136, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002008 DAOQGJ010000000
VERSION     DAOQGJ010002008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134617_length_1010_cov_2.314136"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK683_23290"
     CDS             <1..228
                     /locus_tag="PK683_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11444.1"
                     /translation="TGNLWFWTSEEMQSSSKTGEEKKPVPVLKPSEENPEGPAENRPA
                     PESKKIYNVNMFNGDVFLRESGTLNGLRCIR"
     gene            295..>1010
                     /locus_tag="PK683_23295"
     CDS             295..>1010
                     /locus_tag="PK683_23295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11445.1"
                     /translation="MSRFILILIFSFIHLSAAGRMILHIEPFSAENDETGVWAADAVR
                     AYIQYAVGQMGSVRLSEEEWIRTAYGLEKYKENSLEKEQKVYSRLKKDFILKGSVRRD
                     AYMLKVSMQLSRASDPLKVREFSVSGSLGMMEELKQNLAEGLASAASSIQTREFADFR
                     LTELEKKRLFRNSRISSLGFENLGKSLEKDISYEKSLEFIKKAMETDGNSAEVLIHAG
                     ILFSRRTDLLDESVSALEKAG"
BASE COUNT          300 a          192 c          248 g          270 t
ORIGIN      
        1 actggaaacc tatggttctg gacctcggag gagatgcaga gcagttcaaa gactggggaa
       61 gaaaaaaagc ctgtgcctgt gctgaagccc tcagaggaaa atcctgaagg tccggcagaa
      121 aacagaccgg ctccggaaag taaaaaaata tataatgtaa atatgttcaa cggtgatgtc
      181 tttctcaggg aaagcggaac tttgaacggc ctcagatgca tccgctgatt ttttgcatat
      241 catgagaaaa tattttattt acattccggc tccccgctgc agtcttaact ggtaatgagc
      301 agatttattc ttatactgat attttccttc attcatcttt cagcagccgg aaggatgatt
      361 ctccatatag aacctttttc cgctgaaaat gatgaaactg gtgtttgggc ggctgatgcc
      421 gtaagggctt acattcaata tgcggtcggg cagatgggtt ctgtgcggct gtccgaagag
      481 gagtggatca gaaccgctta cggtctcgag aaatataaag aaaacagtct tgagaaggaa
      541 cagaaagtct attcacgtct gaagaaggat ttcattctga agggatcggt caggcgcgat
      601 gcctatatgc tgaaagtaag catgcagtta tccagagcat ctgatccgtt aaaagtaaga
      661 gagttttctg tcagtggaag tctcggaatg atggaagagc tgaagcagaa tttggcagag
      721 ggtcttgctt ctgctgcatc ttcaattcag acaagagaat ttgcagattt tcggcttact
      781 gaacttgaaa agaaaagact tttcaggaat tcaagaatct cttcacttgg gttcgaaaat
      841 ctggggaaat cccttgaaaa agacatttcg tacgagaaat cgcttgaatt cattaaaaaa
      901 gccatggaga cggatggaaa ctctgctgaa gttcttattc atgccggaat actcttttcc
      961 agacggaccg atcttctgga tgaatctgtt tctgccctcg aaaaagcagg
//
LOCUS       DAOQGJ010002009         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_134716_length_1010_cov_1.641885, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002009 DAOQGJ010000000
VERSION     DAOQGJ010002009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134716_length_1010_cov_1.641885"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PK683_23300"
     CDS             complement(<1..527)
                     /locus_tag="PK683_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNH11446.1"
                     /translation="MKVFVYGGTGLVSGRLIELLLGKGHQVTAGSRNPSAQKKTSGLE
                     WVQTDAFKPELGQNGLNGADAVFLFSPPGYTNQFDILGPWIDSAKKNGVKKVVLMTAM
                     GVDSAPPEAPMRKLELYLESSGLQYVILRPNWFMQNFHTFWISGILKDRKIYFPGGDA
                     KTSFIHSHDISASALA"
     gene            673..>1010
                     /locus_tag="PK683_23305"
     CDS             673..>1010
                     /locus_tag="PK683_23305"
                     /inference="COORDINATES: protein motif:HMM:NF024260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH11447.1"
                     /translation="MNRLKDLILNDWLMDVLSEIISSSGLQGSLLARHSFFGKWGFRF
                     PCERSFGFHIITQGNCFIRHRNTHSLLEKGDIAFIAKGCSHDLVSSPTEKTKDIKYLI
                     RGGRLSATKGL"
BASE COUNT          289 a          231 c          205 g          285 t
ORIGIN      
        1 gcgagagcag atgcagatat gtcgtgagaa tgaatgaagc tcgtctttgc atcgcctcca
       61 ggaaagtaaa tcttcctgtc tttcagaatt ccggaaatcc agaatgtgtg gaagttctgc
      121 ataaaccagt tcggtctgag aattacatac tgaagtccac tggattccag gtaaagttcc
      181 agttttctca tcggagcctc aggaggagct gaatccactc ccattgcagt catcagtacg
      241 actttcttca caccgttctt tttggcagaa tcaatccagg gacccagtat atcaaactga
      301 ttggtatatc cgggaggtga aaacagaaat acagcgtctg cgccgttcag tccgttctga
      361 ccgagttcag gcttgaatgc atccgtctgc acccattcga gtcccgaagt ttttttctgt
      421 gcagaaggat ttctggatcc tgcagtcacc tgatgacctt ttcccagaag aagttcaatg
      481 agcctgccgc tcacaagtcc ggttccgccg taaacaaata ctttcataaa tatctccaaa
      541 atcagtaatg cgtctctcct tttttaatga aaagaccgtt ctgtcttgtt atgaaatcca
      601 tacattcaga atcaagaagt atttttatac ttttaatatc cgaaataaca aaaaacatat
      661 ctaaaagtat aattgaatag acttaaagat ttaattctga atgattggct tatggacgtg
      721 ctttcagaaa tcatcagttc ttccggtctg cagggttcac ttctcgccag acattcgttt
      781 ttcgggaaat ggggattccg ttttccctgt gaaagaagtt tcggatttca tatcattact
      841 cagggaaact gcttcatcag acacagaaac actcacagcc ttctggaaaa aggcgacatt
      901 gcattcatcg ccaaaggctg cagtcacgat ctggtttctt cgccgacaga aaaaacaaag
      961 gatataaaat atctcatccg gggcggccgc ctttcggcaa ccaaaggact
//
LOCUS       DAOQGJ010002010         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_134776_length_1009_cov_4.582809, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002010 DAOQGJ010000000
VERSION     DAOQGJ010002010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134776_length_1009_cov_4.582809"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..>1009)
                     /locus_tag="PK683_23310"
     CDS             complement(16..>1009)
                     /locus_tag="PK683_23310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11448.1"
                     /translation="WMIDYMYMSMDMNEMYVGGKAQDPSKYLIGLQPNYSLSVSGSSG
                     VHNHSGTSSSSSDASPLPSLYAPVLNASPYRYMSVPQKMKMEMTMLSVMKNITDKFSV
                     MIMIPYVNNSMKMLLGNYESSFMRVQGVGDVSVTGVFQFFRKDSHRINLQFGISLPTG
                     SIDEKNLMPLMGESRSPYNMQPGSGTYNTIPGIAYLFSNGKWNAGSFTQVLLRNAKNS
                     NGYRFGNRYESSIWISYSFWEWAAPILRVTYSKWDNISGADSALDYKMDPQNDPNLQG
                     GRRMDILAGMNFTLPSVSEKLKAGLEFGKPVYQHLKGPQMGATTLFNFRLQYMF"
BASE COUNT          293 a          222 c          199 g          295 t
ORIGIN      
        1 aagcagaata tctgtctaaa acatatactg aagtcggaaa ttgaaaagag tggtcgcacc
       61 catctgagga cctttcagat gctgatagac aggctttccg aactccagcc ctgccttgag
      121 tttttcggag acagaaggca atgtaaaatt cattcctgca agaatatcca ttcttcttcc
      181 gccttgaaga ttaggatcgt tctgaggatc cattttataa tcgagggcag agtctgcacc
      241 ggatatattg tcccatttac tgtaagtgac ccttagaatc ggggcagccc attcccagaa
      301 agaataggaa atccagatcg aggattcgta cctgtttccg aatctgtatc cgttggagtt
      361 ttttgcgttc cgaagcagca cctgagtaaa acttcctgca ttccatttac cgtttgaaaa
      421 tagataggca atacccggta ttgtattgta tgttcctgaa ccaggctgca tattgtaggg
      481 agatctggat tcacccatca gaggcatcag atttttttca tcaatggaac ctgtaggcag
      541 agaaattcca aactgaagat tgatcctgtg tgaatctttt ctaaagaact gaaacactcc
      601 tgtaacactc acatccccta ctccctgcac tctcataaag gaactttcat agtttcctaa
      661 gagcattttc atactgttgt tcacataggg aatcataatc attacagaaa atttatctgt
      721 gatatttttc atcacactaa gcattgtcat ttccattttc attttctgag gaacggacat
      781 atatctgtaa ggacttgcat tcagaacagg agcatacagt gacggaagag gacttgcatc
      841 agaagatgag gaagatgttc ccgaatgatt atggacaccg gaacttcctg aaacactcag
      901 actgtagttt ggctgaagtc caatcagata tttggacgga tcctgcgcct ttccgccgac
      961 atacatctca ttcatatcca tgctcatgta catatagtct atcatccat
//
LOCUS       DAOQGJ010002011         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_135071_length_1008_cov_3.050367, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002011 DAOQGJ010000000
VERSION     DAOQGJ010002011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135071_length_1008_cov_3.050367"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PK683_23315"
     CDS             <1..752
                     /locus_tag="PK683_23315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11449.1"
                     /translation="HLCRKHRIPVFRNSRLHLKLYLSENSAYLTTANISERAMADQDS
                     ELFNYELGTRIESLSFEDRLYLQKIVDSSILLDDDIVEEIERQLEAVDSLLDLQDFTL
                     DFGTDDKDFLLSSLPMSEDLEELIRFYREKKAPRGEKLDCLVHDLSLYNIPSGLSEEN
                     CRNLLRERFFRHEFIRAFLDELEMNEGELYFGHVRRWIADNCADCPTPRPFEVTKNVQ
                     ILYHWFDELGEGEFTVSVPGTHSQRIRWNKW"
     gene            complement(759..>1008)
                     /locus_tag="PK683_23320"
     CDS             complement(759..>1008)
                     /locus_tag="PK683_23320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11450.1"
                     /translation="IINVLGREYLIETKIYRYDRQFQEGKTQLAYYCKSLGLTEGVYL
                     VFVPNVILYPERAKEGIEIIEGITIKVFLIRYDEEKDF"
BASE COUNT          260 a          201 c          252 g          295 t
ORIGIN      
        1 atcatctctg caggaagcac aggattccgg tgttcaggaa cagcaggctg catctgaagc
       61 tctatctttc ggagaattct gcatatctta cgactgcgaa catatcggaa agagcgatgg
      121 cggatcagga ttccgagctg ttcaattatg agctgggaac aagaattgag tcgctgagtt
      181 ttgaggatcg tctgtacctg cagaagattg tggacagcag cattcttctg gatgacgata
      241 ttgtggagga gattgaaaga cagctggaag cggtggattc tcttttggat cttcaggatt
      301 ttacactgga cttcgggaca gacgacaagg actttcttct ttcctctctg ccgatgtccg
      361 aagatctgga agagctgatc cgtttttaca gggagaaaaa ggcgcctaga ggagaaaagc
      421 tggactgtct tgtgcatgat ctgtcattat ataatatccc atccggactt tcagaggaga
      481 actgcagaaa tcttcttcgg gaaagattct tccgtcacga attcatacgt gcctttctgg
      541 atgagctgga aatgaatgag ggtgagctgt atttcgggca tgtacgacgc tggattgcag
      601 acaactgtgc agactgtccg acgccgcgtc cttttgaagt tacaaaaaat gtgcagattc
      661 tttatcactg gtttgatgaa ctgggagaag gggaatttac tgtatctgtg cctggaacac
      721 attcgcaaag aatccgttgg aataaatggt aggatttcct aaaaatcctt ttcttcatcg
      781 taacggatta aaaagacttt gattgtaatt ccttctataa tttctattcc ttcttttgca
      841 cgttccggat acaggatcac attcggcaca aatacgaggt agactccttc tgtgagtcca
      901 aggcttttac agtagtatgc gagctgcgtt ttcccttcct ggaactgcct gtcatagcgg
      961 tagatttttg tttcaatcag atattcgcgt ccgagcacgt tgatgatg
//
LOCUS       DAOQGJ010002012         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_135291_length_1007_cov_4.831933, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002012 DAOQGJ010000000
VERSION     DAOQGJ010002012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135291_length_1007_cov_4.831933"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PK683_23325"
     CDS             complement(<1..831)
                     /locus_tag="PK683_23325"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNH11451.1"
                     /translation="MTDSRISSFLKQHSFSEELLPLTPEASVRRYYRVKNSEGTAVLC
                     TDPNFKSEDYPFLLVQKFLKENSFRVPEIIGHSSELGLFLLEDCGDADLSHINDADEY
                     EKYVKNSLDIILKLQELKPVPVISSRSFDTAKLEFEIGHTASGFERLKEKYSFKSDLN
                     FRIMNFLKDTVSYLAAYEDKRITHRDFHARNILLHNNELILIDFQDMMMGVPEYDLTS
                     ILYDAYKKVDMSFREKYYGYFISNLQGKHTKFRERYLYQSLQRSFKALGTYIVMFNDK
                     G"
BASE COUNT          286 a          208 c          190 g          323 t
ORIGIN      
        1 ccccttatca ttgaacataa caatataagt tcctaaagcc ttgaaacttc tctgaagaga
       61 ctgatagaga tacctttctc tgaatttggt atgtttcccc tgcagatttg aaataaagta
      121 tccatagtat ttctctctga aagacatatc cactttttta taagcatcat atagaatact
      181 ggtcagatca tattcaggaa cacccatcat catatcctga aagtcaatga ggatcagctc
      241 attgttatgg aggaggatgt ttctggcatg gaaatccctg tgagtaattc ttttgtcttc
      301 gtatgcggct agataactta cagtatcctt taagaaattc attatcctga aattcaagtc
      361 agacttgaac gaatactttt ccttgagtct ttcaaagccg gaagcagtgt gaccgatttc
      421 aaattccagt ttcgctgtat cgaaagatct ggaagaaatc acaggaacag gcttcagttc
      481 ctgaagcttc agaattatat ccaaagagtt ctttacatat ttttcatact catctgcatc
      541 attgatatga ctgagatcag catctccgca gtcttcaagc agaaaaagtc ccagctcgga
      601 agaatgacct atgatctcag ggactctgaa gctgttttct ttcagaaatt tctgcacaag
      661 aagaaaagga tagtcttcac tcttgaaatt cggatctgtg cagaggactg cagtgccttc
      721 tgagtttttg actctgtagt accttcttac tgatgcttcc ggcgtaagag ggaggagttc
      781 ttcggagaag gaatgctgtt ttagaaaaga tgatattctg ctgtctgtca tagttcacaa
      841 tccgctgtat tcacttcaat gcctttagac agcccgactt tgtcaagtga aatcagtacc
      901 aggccatttc tgaatccacc gtctctatgt attccaaaat acctttgttc agatgggtaa
      961 ttttctgaaa cccgttttcc ttcagaatgc gctcagctgt ttttgat
//
LOCUS       DAOQGJ010002013         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_135376_length_1007_cov_2.785714, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002013 DAOQGJ010000000
VERSION     DAOQGJ010002013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135376_length_1007_cov_2.785714"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PK683_23330"
     CDS             complement(<1..902)
                     /locus_tag="PK683_23330"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11452.1"
                     /translation="MKTQKFFQVFEPSYGKIPSPSFRFFQTVKSQAEPGEKVKFITGS
                     SADIKALYEIEHKGEIIHSEWVDHGKIRKDKNSDQIPYEIAVKEEYRGNFAIHYTYVR
                     SNRLFTRSETFIVPYTNKKLDISFETFRDKLLPGQEEEWRLKIKTGLGEKAAAEMLAT
                     LYDASLDSFRPNSFNFSLYNSMYASKHWQSIKAFDNALSHFLADNWNSYTGSAYLHYP
                     SLNWFGYNFNFYYHYPYRRQRSRNGEIHPPSPSAEAPSMAREEKAMEMDDAPADMKKE
                     AAKPVSTAAPAGAKMDARDKNVDRL"
BASE COUNT          236 a          225 c          215 g          331 t
ORIGIN      
        1 agtctgtcaa cgtttttatc acgagcatcc atttttgctc ctgccggtgc cgctgtggaa
       61 accggctttg ctgcttcttt tttcatgtct gccggggcat cgtccatctc catggccttt
      121 tcttccctgg ccatggacgg agcttcagcc gaaggagaag gtgggtgtat ctctccgttc
      181 ctggaccttt gtcttctgta tggataatga taataaaaat taaaattata cccgaaccag
      241 ttcaaggatg ggtagtgcag atatgcactg ccggtgtaac tgttccaatt gtctgcaagg
      301 aaatggctta atgcattgtc gaaagccttt atgctctgcc agtgttttga tgcatacatt
      361 gaattgtaca gactgaaatt gaaactgttc ggacggaatg aatcgagcga tgcatcataa
      421 agagtcgcaa gcatttctgc cgccgccttt tcgcccagtc cggtcttgat tttcagtctc
      481 cattcttctt cctgtcctgg aagaagtttg tcgcggaatg tttcaaaact gatatccagc
      541 tttttgttcg tataaggaac aatgaatgtc tcgcttcttg taaaaagcct gttgcttctt
      601 acataggtat agtgaatggc gaagtttcct ctgtattctt ccttgacggc aatttcgtaa
      661 gggatctggt cggagttctt gtctttcctg atctttccgt gatccaccca ctcactgtga
      721 atgatttctc ctttatgctc aatttcataa agtgctttaa tgtcagcact tgagcctgta
      781 atgaatttta ctttctcgcc cggttctgcc tgacttttta ctgtctgaaa aaatcggaat
      841 gagggagaag gaatctttcc atacgaaggt tcaaatacct ggaagaattt ctgagttttc
      901 aatcttatta cccgagtcaa tcgtgaatac ggtgtaagcc tcacaatggc caaagtgttc
      961 gtcaacaaca ccgtttcttg ttggtacagc aaccttcata acatttc
//
LOCUS       DAOQGJ010002014         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_135605_length_1006_cov_2.921136, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002014 DAOQGJ010000000
VERSION     DAOQGJ010002014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135605_length_1006_cov_2.921136"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..>1006)
                     /locus_tag="PK683_23335"
     CDS             complement(262..>1006)
                     /locus_tag="PK683_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11453.1"
                     /translation="PAFVKYVIMTPRAQVIKNLENHIGKIESKLGADAGGRNLLRRFL
                     MVGRILQERAEKEYGIEVTVEDYNYRIGKIRHTILDRLAEKMHLKHYDFNADAIHKLR
                     LITTTLELIEVNYPSPDLPKLSARELEYISKECIKAYDFIVMKPDYLISYPTPERFYE
                     WLQRFESLVLGATPRMSGGEPHHEPRKAHMFFGKTYKFGDYSETYKKNKTEVVTRFLS
                     DLRTEMQSMLDESQSLTSPIVEPGDIGGT"
BASE COUNT          263 a          223 c          184 g          336 t
ORIGIN      
        1 agtccagctt cattgttttt cgaggtcaaa tcaaaacgat aaatattggc atatttttca
       61 ttttcctttg aaccggaaaa tgactcaaag catcgatcaa agatccgttt aaattctgcc
      121 ttgcgatttt tttgcaagga atggcagcca gaactgataa ttaaaaataa tgtattcata
      181 tccttggcag ctgtatacgg taatccggct tagtcaggtc gcattacttg catctgcatc
      241 catttactga atcccgactg tttaagtgcc ccctatgtct cccggttcca caatcggaga
      301 agtcagcgac tgagattcat cgagcattga ctgcatttcc gttctcaaat cactcaagaa
      361 tcttgtcaca acttcagttt tattcttctt gtaagtttca gaataatcgc cgaacttgta
      421 tgtttttccg aaaaacatat gtgcctttct tggttcatgg tgaggttctc ctcctgacat
      481 tctgggtgtc gcacctaaga ccaaggattc gaatctttgg agccactcat aaaatctttc
      541 aggtgtagga taactgatca gataatccgg tttcataacg ataaaatcat atgctttaat
      601 acattctttg ctgatgtatt ccaattccct tgcgcttaat ttcggcagat caggagaagg
      661 gtagttcact tcaatgagct cgagtgttgt agttatcagc cgcaatttgt gaatggcatc
      721 cgcattaaaa tcataatgct tcagatgcat tttctcagcc agtctgtcca ggattgtatg
      781 acgaattttc cctattctgt aattataatc ttccacagtg acttcaatgc cgtattcttt
      841 ttcagctctt tcctgaagga ttctcccgac cattaaaaag cggcgcagta aatttcggcc
      901 tccggcatct gcacccagct tcgattcaat ttttccgata tggttttcca ggtttttaat
      961 cacctgagct cttggggtca ttatcacata ttttacaaat gcaggc
//
LOCUS       DAOQGJ010002015         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_135634_length_1006_cov_2.628812, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002015 DAOQGJ010000000
VERSION     DAOQGJ010002015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135634_length_1006_cov_2.628812"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1006
                     /locus_tag="PK683_23340"
     CDS             <1..>1006
                     /locus_tag="PK683_23340"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNH11454.1"
                     /translation="FITDPIVLSPDHSIEDLDHIKFTHGFSGIPITEDGTMRTKLIGI
                     VTNRDIDFETDRTQKLGKVMTKDLVTGQVGISLKEANNILRSKKIGKLPIVNKDGKLA
                     ALVSRSDLKKNKEFPDASKDSNKRLRVGAAVSTLPESRDRVAALVAAGVDVIIIDSAQ
                     GNSVYQIEMIQFIKEKFPGLDVIGGNVVTMEQCRNLIEAGADGLRVGMGPGSICITQD
                     MMAVGRAQATAVYKTGNYSKKFGIPIMADGGISSIGDISTALAIGASTCMMGSMFAGT
                     SEAPGEYFYENGIRLKKYRGMASLEAMKAGGDKRYFSEGQKIKVAQGVSGAVVDKGSV
                     "
BASE COUNT          313 a          198 c          271 g          224 t
ORIGIN      
        1 gatttatcac tgatccaata gtcttaagtc ccgaccattc aatcgaagac cttgaccata
       61 taaaattcac tcatgggttt tccggaattc cgatcactga agacggaact atgaggacaa
      121 aactcatcgg gatagtcaca aacagagata tcgattttga gacagacaga actcagaaac
      181 tcggcaaggt aatgactaag gatcttgtga ctggacaggt aggaatcagt ctgaaggaag
      241 caaacaatat tctccggtcc aagaagattg gaaaactccc aatcgtgaac aaggacggca
      301 aacttgcagc tcttgtcagc cgttctgatc tgaagaagaa caaggaattt ccggatgcat
      361 ccaaggacag caataaaaga ctcagagtag gcgctgctgt ttccactctt cctgaatcga
      421 gggacagggt tgctgcactt gttgctgcag gagtagatgt catcattata gattcagcac
      481 agggcaattc agtataccag attgaaatga ttcagttcat caaggaaaaa tttccaggtc
      541 tggatgtgat tggagggaat gtagtgacaa tggagcagtg cagaaatctg attgaggcag
      601 gggctgacgg actgcgagtg ggaatgggtc caggttccat ctgcatcact caggatatga
      661 tggcagtcgg aagagcacag gcgactgcag tctacaagac agggaactat tccaagaagt
      721 tcggaattcc gattatggca gacggaggaa tctcaagcat tggagacatt tccacggcac
      781 tcgcaatagg agcatctacc tgcatgatgg gttccatgtt tgcaggtaca agcgaggctc
      841 ccggagagta tttttatgaa aacgggatca gactgaaaaa atatcgcggg atggcaagtc
      901 ttgaagctat gaaggcaggc ggagacaagc ggtatttcag tgagggacag aaaatcaagg
      961 tggctcaggg agtcagcggt gcagtcgtag acaaaggatc agtcat
//
LOCUS       DAOQGJ010002016         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_135913_length_1005_cov_2.575789, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002016 DAOQGJ010000000
VERSION     DAOQGJ010002016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135913_length_1005_cov_2.575789"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PK683_23345"
     CDS             complement(<1..778)
                     /locus_tag="PK683_23345"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH11455.1"
                     /translation="MQTQSMFEFSKDDSLTGFRLQRLEVLNWGTFHGKIWKIEPLGFN
                     SLLTGDIGSGKSTLVDALTTLLVQHNKITYNKAAGAEGKERNLYRYIRGAYANTRGEE
                     LSAKPQYLREKDSHTVLLASFYNDGYGQKYTLAQVFWLRDDEVQKMYIISSGDLNIRE
                     HFTGFGTEISELKKRLKKLQGTEVLDSYKEYSARFSNYFGIKSEKAMDLFYQTVSLKS
                     VNNLDEFVRVNMLEKREEETKSEIEGLKKNFEDLNIAHEAI"
     gene            complement(756..>1005)
                     /locus_tag="PK683_23350"
     CDS             complement(756..>1005)
                     /locus_tag="PK683_23350"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HNH11456.1"
                     /translation="MSRTEIKEAAKIFFPHETNETKLLDKLDAAINKLTYEYHFLKEM
                     NGNKERLEVKRIIKAVIDAEKLLEIKEKLLHYADSVDV"
BASE COUNT          248 a          237 c          184 g          336 t
ORIGIN      
        1 ggatggcctc atgggcaatg ttcagatctt caaaattttt cttcaggccc tcaatctcgg
       61 attttgtctc ctcttctcgc ttttccagca tattgacccg gacaaactca tccagattgt
      121 tgactgattt caaggatact gtctgataga aaagatccat tgctttttcc gacttaatgc
      181 cgaaataatt agaaaatctt gcagaatatt ctttatacga atccagaacc tctgttccct
      241 gaagtttttt cagccttttc tttagttcag aaatctctgt tccgaatccg gtaaaatgct
      301 ccctgatatt cagatcaccg gaggagatga tatacatttt ctgcacctca tcatctctca
      361 gccagaaaac ctgcgccaag gtatatttct gtccgtatcc gtcattataa aatgaggcaa
      421 gcagaacggt gtggctgtcc ttctcccgaa gatactgcgg tttggcagac agttcctcac
      481 ccctcgtgtt tgcataagca ccccggatgt atctgtacag atttctttct ttcccttcgg
      541 ctcctgccgc tttattataa gtaattttat tgtgctggac aagcagagta gtcagtgcat
      601 ccacaagggt ggacttcccg gaaccgatat cacctgtgag gagtgaattg aatccaaggg
      661 gctctatttt ccagatttta ccgtgaaatg ttccccagtt gaggacctcc agcctctgca
      721 gcctgaatcc tgtcagagaa tcatcttttg aaaattcaaa catcgactga gtctgcatag
      781 tgcaaaagtt tctcctttat ctccagaagt ttttcagcat caatgactgc cttgatgatt
      841 ctttttactt caagtctttc tttatttcca ttcatttcct tcaggaaatg atactcgtaa
      901 gtaagcttat ttatagctgc atccagttta tccagaagct tggtttcatt tgtttcgtga
      961 ggaaaaaata ttttcgcagc ttccttgatt tcagttctgc tcatc
//
LOCUS       DAOQGJ010002017         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_135923_length_1005_cov_2.447368, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002017 DAOQGJ010000000
VERSION     DAOQGJ010002017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135923_length_1005_cov_2.447368"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PK683_23355"
     CDS             complement(<1..419)
                     /locus_tag="PK683_23355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11457.1"
                     /translation="MPADIFSQDQKSVQQKLLVINSYLPHRSGKQDSVAGEIQSSLKK
                     ELEQHGYEVAVISEPRASGLQTARQMNAAFYAEGYFRTSSNGNLSIFTQIYNPDTGNI
                     IDAVSVSDEAEAVDGLKLDENENKIPKEKTISEHTKKT"
     gene            745..>1005
                     /locus_tag="PK683_23360"
     CDS             745..>1005
                     /locus_tag="PK683_23360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11458.1"
                     /translation="MLFNLPPRQKLNLSIIISFFLFNYCTGTYYGVSGSAVYSRNEAV
                     AKLNKALLLKTAACGANEKNSSAVYAVISQSASKTLDGAYYTK"
BASE COUNT          302 a          200 c          186 g          317 t
ORIGIN      
        1 gttttttttg tatgttcaga aattgtcttt tctttgggaa ttttattttc attttcgtca
       61 agctttaagc cgtctacagc ctctgcttca tctgaaaccg aaaccgcatc aataatgttt
      121 cctgtatcag gattgtaaat ctgagtgaat atgctgagat tgccgtttga agaggttctg
      181 aaataacctt ccgcataaaa cgcagcattc atctgccggg ctgtctgaag accggaagcc
      241 cttggttcgg aaattacagc aacttcatat ccgtgctgct caagttcctt tttcagagag
      301 gactgaattt caccggcaac agaatcctgt tttcccgatc tatgcggcag ataggaattg
      361 attacgagga gtttctgctg aacactcttc tgatcctgtg aaaaaatatc ggctggcata
      421 ttcaaatata tgcaaatact cagtgtcatg agaattctaa atccagtcat ttatttcctc
      481 tgctataaat tatcattata aaccctgctg tcaatgcggc cgcaactgat gaaggaatca
      541 aagccttttc tgcgcatttt gcatattcgg tctaaggtca atactggcaa tcaaaattgt
      601 ctgaacgggt atttttttta ttgataaata tcaaaactgt cattttcaca gatcagtaat
      661 taatcccttt aatcataatt cgaaaaatct gcagaaagaa aaaaattaat tgaaaagaat
      721 tctgatccct taaaaatctg ctgtatgctt ttcaatttac cccccagaca gaaattgaac
      781 ctgtccatta taatatcatt ttttctgttt aattactgta caggcactta ctatggtgtg
      841 tcgggctctg cagtctacag cagaaatgaa gcagtggcta aactgaacaa ggcactttta
      901 ttgaagacag ccgcctgcgg agcgaatgag aaaaacagtt ctgcagtata tgcagtaata
      961 tctcaaagtg catcgaaaac actggatggg gcgtattata ccaaa
//
LOCUS       DAOQGJ010002018         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136000_length_1005_cov_1.984211, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002018 DAOQGJ010000000
VERSION     DAOQGJ010002018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136000_length_1005_cov_1.984211"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PK683_23365"
     CDS             <1..734
                     /locus_tag="PK683_23365"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH11459.1"
                     /translation="PIGFLAGLFIYCRMLKLDFFHFSSPFLKASLLWLGIGRIACFLN
                     GDAYGIPTSSFLGVTFSEDSSDLMSEWKLLSSGYSFSPNPLPEIEKIFTAQYGLHLSD
                     LPIPDKLSNLKMTGYSTLADLNSFYKNSSCIKTLAEQGLWPFPTVYPKVHPTQLSELF
                     SLVGIYFFFEYFYGKRWRESTIPFLYIIIFGKNRFWIEFFRADRSPGFFGLTGAQILC
                     LGYTLSGICMYFYYTEKWKKDGIPL"
BASE COUNT          267 a          201 c          199 g          338 t
ORIGIN      
        1 ctccaatcgg gtttctggct ggtctgttca tttactgcag gatgctgaaa ttggatttct
       61 ttcatttttc atctcctttt ctcaaggctt cactcctttg gcttgggatt ggaaggattg
      121 cctgtttctt gaacggtgat gcttatggaa ttcctacaag cagtttttta ggggtcacat
      181 tttcagaaga cagttcagac ttgatgtcag aatggaaatt actgagttca ggatactcat
      241 tcagtccgaa ccctcttcct gaaattgaaa aaatctttac tgcacagtat ggtcttcatc
      301 tttccgatct gccgattccg gataagctct ctaatcttaa aatgacgggc tattcgactc
      361 ttgctgatct gaacagcttc tataaaaaca gttcctgcat taagacgctt gcagaacagg
      421 gactttggcc atttcccacg gtttatccaa aggtacatcc gactcagctc tctgaacttt
      481 tttcgctggt cggcatatat tttttctttg aatattttta tggaaaaaga tggagggaaa
      541 gtacgattcc ctttctttac attatcattt tcggaaagaa ccgtttctgg atcgaatttt
      601 ttcgcgcaga cagaagcccc gggtttttcg gacttacggg agcacagatt ctctgcctgg
      661 gatacacatt gtccggaatc tgtatgtatt tttattatac agaaaaatgg aaaaaagacg
      721 gaatacctct gtaaattttt tcagcagcat actgcagtgt ctcttttgtc tcattataat
      781 aaaagccgtc agtcagcttt caaaaatgac ttgccagttt taaatgattc ctaagatcat
      841 ttatgaactg aagaagcatt tatacaggag attttttgta aaatcctgca atacttccag
      901 ccaagaggtt tttgtaaaat ggacgatttt gtcagaaaag aagtcaatca gctgaatatc
      961 aggatggaac tgttcagtta tttcggagcc gttcctctcg gttcc
//
LOCUS       DAOQGJ010002019         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136194_length_1004_cov_2.613277, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002019 DAOQGJ010000000
VERSION     DAOQGJ010002019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136194_length_1004_cov_2.613277"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PK683_23370"
     CDS             complement(<1..321)
                     /locus_tag="PK683_23370"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNH11460.1"
                     /translation="MIVSFVQTKGGTGKSTLSINTAFSKTAEERFQSIALVELDPQGT
                     LQSWWQSRNDEGRSPGKVSFHHISSTQKEVIQSGITSIAEHNELLILDIPGESTSKLH
                     TKFAC"
     gene            490..813
                     /locus_tag="PK683_23375"
     CDS             490..813
                     /locus_tag="PK683_23375"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH11461.1"
                     /translation="MKKLPIGIQTFSKITAGDYSYADKTKMIAEPADRSGYNFLSRPR
                     RFGKSLFPDTLKEAFEGNKELFQGPYPKKKWNWDKKYPVLRLDLSLAFSRKSRNIINY
                     EWETV"
     gene            798..>1004
                     /locus_tag="PK683_23380"
     CDS             798..>1004
                     /locus_tag="PK683_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11462.1"
                     /translation="MGDGLIMVSGKKIPSREKLSWDTIEYIENVPNHFLIPEHHWPNF
                     QKTSLSLKEKIRLNHFACFFMCEMF"
BASE COUNT          298 a          216 c          206 g          284 t
ORIGIN      
        1 acaggcaaat tttgtatgca gtttgcttgt actttctccc ggaatatcga gaatcagaag
       61 ctcattgtgc tctgcgatgg aagtaattcc cgactgaatg acttctttct gtgtacttga
      121 aatatggtga aaggaaacct tgccgggact tctgccttca tcatttctgg actgccacca
      181 gctctgcaat gtaccttgcg gatccagctc cacaagggct atggactgga atctttcttc
      241 agctgttttg gaaaaagcgg tgttgatgga cagggtgctt tttcctgtcc cgcccttcgt
      301 ctgcacaaaa cttacgatca taggtcaagt tttttttgag aaagccctaa gtcaagggaa
      361 aagagcctga ttgaacctcg atttccatgt actgccctgt aaatgcagat ttcatcaaaa
      421 atgattcatt gaatcagggc agggaaaact ccgcttgaca cacattctca taatattgac
      481 cttattcctg tgaagaaact tccgatcgga atacagacat tttccaagat caccgccggt
      541 gattattcct atgctgataa aacgaaaatg atagcggaac ctgccgacag aagcggatac
      601 aatttccttt cccgtccgcg ccggttcgga aaaagtcttt tccccgatac tctgaaagaa
      661 gcctttgagg gaaacaagga actctttcaa gggccatatc cgaaaaagaa atggaactgg
      721 gataaaaaat atcctgtcct tagacttgat ttatccttgg cattttccag aaagagcaga
      781 aatataataa actatgaatg ggagacggtc tgattatggt ttcagggaaa aaaataccct
      841 cacgcgaaaa actcagctgg gatacaatag aatacattga aaacgttccg aatcattttc
      901 tgattccgga acatcattgg cctaattttc agaaaacctc tctatcatta aaggaaaaaa
      961 tcagactgaa tcattttgcc tgtttcttta tgtgcgagat gttc
//
LOCUS       DAOQGJ010002020         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136235_length_1004_cov_2.246575, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002020 DAOQGJ010000000
VERSION     DAOQGJ010002020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136235_length_1004_cov_2.246575"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1004
                     /locus_tag="PK683_23385"
     CDS             <1..>1004
                     /locus_tag="PK683_23385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11463.1"
                     /translation="SDMSVQYVTGLTGAGTRFTVPSSKTEQKFRNGSLFRTVNSSVSY
                     DAYGNPTGTVISSDGHTEETSAAYINDWTFDTYIEGLPETSVKKINGAVQEEKSFTYD
                     SMRRPVSVTLFPSSSPRTVSTSYDSKGQPSLITDVLGYTVSHIYGQSAGNCLSESKNP
                     LGHVSRTVCNEENGTEVQMTDPNGAVTQKLYDEYGRPVGTILPGNSQTSEETSYEFTQ
                     SPFGHVVRKRVRDDVNGWIESAEFFDAKGRSIRKESPGIGGMKFVELTEYDSAGRTAR
                     KSDSFLEGSESPLWSVYSYNADNETVSVSLPDGGRADSSISLSGSNVISSSTVYSPSG
                     V"
BASE COUNT          297 a          213 c          253 g          241 t
ORIGIN      
        1 tcagatatgt cagtccagta tgtcacagga ctgaccggtg caggaaccag gttcactgtc
       61 ccttcttcta aaacagaaca gaaattcaga aacgggagtc tgttcaggac agtgaattct
      121 tcagtcagct atgatgcgta cgggaatccg actggaacag tcatatcctc tgacggacat
      181 actgaggaaa cttccgcagc atatatcaat gactggacat ttgatacata tattgaagga
      241 cttccggaaa cttccgtaaa gaaaatcaac ggtgcagttc aggaagaaaa aagtttcacc
      301 tatgattcaa tgagaagacc agtttccgtg actctttttc cttcttcttc tccaagaaca
      361 gtttcaactt cttacgattc taaaggacag ccttccctca tcacagatgt actgggatat
      421 acagtatccc atatctacgg gcagtctgca gggaactgtc tttctgaatc gaaaaatccg
      481 ctggggcacg tcagccggac tgtctgcaat gaagaaaacg ggacagaggt gcagatgacc
      541 gacccgaacg gtgcagtgac tcagaaactg tatgacgaat acggaagacc tgtcgggaca
      601 attcttccgg gaaacagtca gacttccgaa gagacgagtt atgaatttac acagagtcct
      661 ttcggtcatg ttgtcaggaa gagggtgcgg gacgatgtaa acggatggat agagtctgcg
      721 gaatttttcg atgcaaaagg caggagcata agaaaggaaa gtccgggaat cgggggaatg
      781 aaatttgtgg aactgacaga gtatgattct gccggaagaa cagcaagaaa gtcggattct
      841 ttcctggagg gaagtgaaag ccctctctgg tctgtctatt cgtacaatgc ggacaatgaa
      901 actgtttctg tcagtctgcc tgacggcgga agagcagaca gcagtatatc attgtcaggg
      961 tcaaatgtaa tcagcagttc cactgtatat tccccgtcag gcgt
//
LOCUS       DAOQGJ010002021         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136237_length_1004_cov_2.237092, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002021 DAOQGJ010000000
VERSION     DAOQGJ010002021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136237_length_1004_cov_2.237092"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1004)
                     /locus_tag="PK683_23390"
     CDS             complement(<1..>1004)
                     /locus_tag="PK683_23390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH11464.1"
                     /translation="RERSDKLKTDILNEYRNGLRTKIEVLDAESKFRASELALQKSRS
                     DYRLSVFELQNQTGISESIVPSERLFWDYIFLAPAEGRRRSEIFSMKDRPDLKKADAA
                     LMKAKLEKENAENYWKPKFYIGGYAGKNSNDGTLPKHETYGLNFSLVLPFGSSSSQTN
                     GNFGVQKDGTGIQRIPNYGPQFVGQGENSFFNSNLGLFENFTHSRKILEGEIQMQEIQ
                     LQKSILEKQLETEKERLSLKLDDVFSSVRTANSRLALQFEILRVTSLKMKNGQARKLE
                     LMNAESEFLKSADELAEALGEYLKTAFEFQAVLGIDAPCFFADTHKDGSNILLKKMID
                     G"
BASE COUNT          241 a          234 c          193 g          336 t
ORIGIN      
        1 ccgtcaatca ttttcttcag cagaatattg cttccgtcct tatgtgtgtc ggcaaaaaag
       61 cagggtgcgt caattccaag cacggcctga aattcaaatg ctgtcttcag atactctccc
      121 aaggcttcag ccagctcgtc cgcagatttc agaaattccg attctgcatt catcagttcc
      181 agttttcttg cctgcccatt tttcattttc agtgaagtga ctctcagaat ttcaaactga
      241 agagccagtc tggaattggc tgtcctgact gaagaaaaaa catcatccag tttcagagac
      301 agtctctctt tttctgtttc cagctgcttt tcaaggatgc ttttttgaag ctggatttcc
      361 tgcatctgga tttctccttc cagtattttt ctggaatgcg tgaagttttc aaaaagtccc
      421 aggttggaat tgaaaaagct gttttctccc tgtcctacaa actgcggacc ataatttgga
      481 attctctgta ttcctgtgcc gtctttctga actccaaaat taccgtttgt ctgcgaagaa
      541 gaactgccaa acggaagaac aagagagaaa ttcaatccgt atgtttcgtg tttcggaagc
      601 gtgccgtcat ttgagttttt gcctgcatag ccgcctatgt aaaatttcgg tttccaatag
      661 ttttcagcat tttccttttc cagttttgcc ttcatgagag ctgcatctgc tttcttcaga
      721 tcaggtctgt ctttcattga aaaaatctca gatctccttc tgccttcagc cggagccaga
      781 aatatataat cccagaaaag tctttccgaa ggaacaatgc tttcagatat tcctgtctga
      841 ttctgaagtt caaagacgga aagcctgtag tcagatctgc ttttctgcag tgcaagctcg
      901 gatgccctga actttgattc agcatccagg acttcaattt tcgttcttag cccgtttctg
      961 tattcattca gaatgtctgt cttgagttta tcggaacgtt ctct
//
LOCUS       DAOQGJ010002022         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136256_length_1004_cov_2.132771, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002022 DAOQGJ010000000
VERSION     DAOQGJ010002022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136256_length_1004_cov_2.132771"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PK683_23395"
     CDS             complement(<1..474)
                     /locus_tag="PK683_23395"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNH11465.1"
                     /translation="MLLDLLNSFPEFSGKKQIRNLQVMNPELPKSFKDDKLSILDIRA
                     EDISGNKFLIEMQASHQAYFTKRVMYYWSSAYSKAIRKGQNYDRLPRIYSFNFTSFVL
                     FQGQTHFHSVFEVKEKNNPEICLTEVLEIHIVELPKLEKLLPELQTDDIPEDARLL"
BASE COUNT          300 a          173 c          216 g          315 t
ORIGIN      
        1 aagtaatcga gcgtcttcag gaatgtcatc agtctgaagt tcgggaagaa gtttttccag
       61 cttgggcagt tctacgatgt gaatttccag aacttcggtc aagcagattt ccggattgtt
      121 tttttccttg acttcaaaaa cagaatgaaa atgagtctga ccttgaaata agacaaaaga
      181 agtgaaattg aaggaataga tcctagggag tctgtcataa ttctgtcctt ttcgaattgc
      241 ttttgaatac gcactcgacc aatagtacat aactcttttt gtgaaatatg cctgatgaga
      301 cgcctgcatt tcaataagaa atttatttcc tgaaatatct tcagcgcgaa tatccaaaat
      361 cgatagttta tcgtctttga aagatttcgg cagctccggg ttcatcacct gcaaattcct
      421 gatttgcttt ttaccggaaa attcaggaaa tgaattgaga agatccagaa gcagatcttc
      481 attttttggt aactactcag taggaaatat tttgtaagat aatttttgaa gaaaagcttg
      541 aaaaagcggg gtcttagggg ctgcaagccc ctcgcaagct tggaagcggc taattttccc
      601 gatagggatc acttttcttg ctttctaaaa gcaaattgta attacgaagc cgtcaggctg
      661 agtagttaca attttttgca aaaatggatt tgaaaacaaa gtcgttggat aatggcagga
      721 gcctcataag aaagtaatct ttttatggta tatttttgta tagcattttt tttgatgaag
      781 attatttaaa aatggcagtg ttatgatgag tccttcaaaa tataacatca gtatctttcg
      841 atatgcaaga atggaagtcg ccgcaaacct ggaagtcgct gaagtaactg atctttgatc
      901 cctgtgcctg cacctccgcg atacggacag tgcaggaggg ttatcttctc agtttccgaa
      961 actggctctg gtgacggtta ttccagttgg tgtagaagaa ttag
//
LOCUS       DAOQGJ010002023         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136308_length_1004_cov_1.774499, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002023 DAOQGJ010000000
VERSION     DAOQGJ010002023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136308_length_1004_cov_1.774499"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          307 a          203 c          170 g          324 t
ORIGIN      
        1 ggccctgctg aaagacctgt ggaagagaaa gtgaaaactg ccaacgccaa tactaaactg
       61 taaatcttat ttttcatttg aaactccttt caattatgca tttctgcact ggaaacaatg
      121 ttagaaactt ttgatgaatg tcaaatattt tttttaatca gtaaagattt tttaatcatt
      181 ttttactgat tctgatcgtc aaaaagaata ctgcattata attgacagta tgtgtgcata
      241 ctaaactgcc ggggattgtc ctcaccctgg atttccttgc ataaattcat aatttgcatt
      301 ccttactgat tttctgacat atacttattc tcttagtctg cagtatttcc atatttctta
      361 tctaattggc aaaggtgctg caaatgcatt ttcactgctg actaaaaata gcaatttcag
      421 tataagaata ctcttcaatc tgtctactta tacagatcag cgaaagaaat agccatagtt
      481 tgcttcctta agaaggtgaa atgttcactg aatttcaaat atcagcagag tttttttaac
      541 aggtactgat ttcattcagt caactggagt ttttcggttt gtccaatatt ccgcagagcc
      601 tgttactgaa aaacacctgc tttcaagttt aagggaattc agatgccgga ctgacggtac
      661 agccgttcag tcctatccga ttgcaccgca ctgtccagtc agattttact gcgcagcatc
      721 ccctgaatat tcaaaaaaat atatattaat aaattttata atattctgat cgctcatctc
      781 tcccaaaaga agcagagagg ccaaactgcc ggaaaccaat tttttatgaa tctgttccgc
      841 cgggcagtgt tttcataaaa cgtgcattca gattattttt ttgaagacaa atatagaaga
      901 gcgattgtcc atccgacaac aaactgaatc ggaaaagtaa taatgtccag cagaactccg
      961 cccgcaggca cgatgatcct tgcgatcagc gacgcttctt ctgt
//
LOCUS       DAOQGJ010002024         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136383_length_1003_cov_5.216245, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002024 DAOQGJ010000000
VERSION     DAOQGJ010002024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136383_length_1003_cov_5.216245"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..652)
                     /locus_tag="PK683_23400"
     CDS             complement(80..652)
                     /locus_tag="PK683_23400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11466.1"
                     /translation="MKKIYRIIAYTVVLFSLMFSVCRKNQAESEEDRISRIKADYRSR
                     IRTEYVIEKSASSREDAVRKFLESISLLKTGAEPDFYCSEKEYKEIFLPNTVDENTLT
                     SFMNAEEAWKITKIRRDLAFTDMNALLKGKNFTIKEFIWSEKQRELHSLKGHVLKKVI
                     LQSGKDEIEIDSVRLVIEHRNQFKVCVVAK"
BASE COUNT          279 a          234 c          159 g          331 t
ORIGIN      
        1 gcagaaaaaa ataaaaaaaa ccaaagcttc acagcttttg tatgtaattt taatgattga
       61 agaactttct tcaacgtttt cattttgcca ccacgcatac tttaaattga ttcctgtgtt
      121 ctatcacaag tcggacagaa tctatctcga tttcatcctt gccggactgc agaataactt
      181 ttttgagcac atgtcctttc aggctgtgaa gttctctctg tttttcagac catataaact
      241 cttttattgt aaagtttttt ccttttaaaa gagcattcat atcagtgaat gcaagatccc
      301 gtctgatctt tgtgatcttc catgcctctt ccgcattcat aaaagaggtg agggtattct
      361 catcgactgt attcggaaga aaaatctcct tgtattcctt ttcactgcag taaaaatccg
      421 gctcggctcc cgtcttgaga agtgaaatgc tctccagaaa ttttctgaca gcatcttctc
      481 ttgaagacgc ggacttttca ataacgtact cagtacgtat tctgcttctg tagtctgcct
      541 ttattctgct gattcgatcc tcttccgatt cagcctgatt tttcctgcac actgaaaaca
      601 ttaaactgaa taaaacaaca gtatatgcta ttattctata tatttttttc acccatttac
      661 cctcattaat attctcctgt ccctgctttg cagttttaca aaaaaccgaa gaagataaaa
      721 tccctgaaga tttctcttca ggggaatgtc tgattcagaa gacagaaaac cgcagtattt
      781 ccaaaacata ttcgaatcac tgcaggtgcg aagattcatc cgcacctctg tttcaaatcc
      841 agtatacaga gacattatat attatctcgc tctgtataca gtccagtctg cccaccattt
      901 gccagtcgct gaagttcctc ctcctgcacc ggaaactgtc gaagtataat ctggtttagt
      961 cgaaccgaac ccgcagtcag ttgcaacatt tccttcagta gtc
//
LOCUS       DAOQGJ010002025         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136391_length_1003_cov_4.011603, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002025 DAOQGJ010000000
VERSION     DAOQGJ010002025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136391_length_1003_cov_4.011603"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..400
                     /locus_tag="PK683_23405"
     CDS             218..400
                     /locus_tag="PK683_23405"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNH11467.1"
                     /translation="MDSLARKFLTEEEYLNLERNSSTKNEFFRGEMFALAGAKEKHNL
                     IVTNLIRDISTALKKD"
     gene            complement(421..834)
                     /locus_tag="PK683_23410"
     CDS             complement(421..834)
                     /locus_tag="PK683_23410"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH11468.1"
                     /translation="MNKKPDFNEALSRLLDSITNRKLDVYKSFLTKGNLLYTIVQNGY
                     AFKTPQELFAMHEEWFKDQNWIWEGTPVNTAVGEDMAFALIKYEYRAKKEDVPFSTWL
                     TYVFRLEDGEWRLIHDQNTALDFNAFAKSAGLNSD"
BASE COUNT          313 a          203 c          189 g          298 t
ORIGIN      
        1 ttagtcaaat tttttacttc aatcattgag tcctcattct taatgaccaa agtataaaaa
       61 ggcgaatgtc tgaaaaatga aaatttttct caacttaatg aaaagtctgg gattcgggca
      121 ggtcagaagt tctgcaaaaa agcacccgct gacttgacaa catctgtaaa tcgattttct
      181 cttgacagaa cgaaatgcca ctgtcttctc ttaagttatg gacagccttg cccgaaaatt
      241 tctgactgaa gaagagtatc tgaatcttga aagaaattcc agtacaaaaa atgagttctt
      301 tcgcggagaa atgttcgctt tggcaggggc gaaagaaaaa cacaatctga tcgtaacaaa
      361 tctgatcaga gacataagca ctgcactgaa gaaagactga aaaacgggga ttgatctgag
      421 tcagtcagag tttaagccgg ccgattttgc aaatgcatta aaatcgagtg cagtgttctg
      481 gtcgtggatc agacgccatt ctccgtcttc aagcctgaag acataagtca gccaagtgct
      541 gaacggaaca tcttcctttt ttgcacggta ttcatatttg atgagtgcaa atgccatatc
      601 ctcaccgaca gctgtattga ctggagttcc ttcccatatc cagttctgat ccttaaacca
      661 ttcctcatgc atagcaaaaa gctcctgcgg tgtcttgaag gcatatccgt tctgaacaat
      721 cgtatacagt aaatttcctt tcgttagaaa tgacttatat acatccagtt tccgatttgt
      781 aattgaatcc agaagacggc tgagtgcctc attaaaatca ggttttttat tcatacattc
      841 atcctcaaag cttctattga ataaaaagtc tttctttgaa tcaagaaaaa tattctggaa
      901 ttttcgggtg cttgcagccg tccctaattc tctcaagata aggatcattc accacgtatt
      961 gacaatgata atgaatcaaa tttcatattg atctgtggca gtc
//
LOCUS       DAOQGJ010002026         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136464_length_1003_cov_2.566456, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002026 DAOQGJ010000000
VERSION     DAOQGJ010002026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136464_length_1003_cov_2.566456"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PK683_23415"
     CDS             complement(<1..781)
                     /locus_tag="PK683_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNH11469.1"
                     /translation="MAKMTYREALNRAMCEEMEKDPNIFLMGEEVGHYQGAYKVSQGM
                     LDKFGEKRVVDTPIAENGFAGIGVGAAMLGLRPIIEFMTWNFSLVAIDQIVNSAAKMR
                     YMSGGQFSMPIVFRGAGGAGGRVAAQHSQCFEAWYANVPGLKVIAPATPKDACGLLKT
                     SIRDNNPVIFIESEVLYGTSGEVPDNEYSIPLGKGEIKKHGEDISIITWSRALEFSIQ
                     AANELEKEGIRCEVVDLRSIRPLDEEIIYESVRKTNKAIIVE"
     gene            complement(801..>1003)
                     /locus_tag="PK683_23420"
     CDS             complement(801..>1003)
                     /locus_tag="PK683_23420"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH11470.1"
                     /translation="KEEVDTYKRKDPLLRARADLIDGQVSEEELLKIEEEIEEIVNES
                     VEFADASPEPPPAALWQHVYAD"
BASE COUNT          231 a          274 c          195 g          303 t
ORIGIN      
        1 cttccacgat gatcgcttta tttgtctttc tcactgattc atagatgatt tcttcatcga
       61 gaggacggat gcttctgaga tccactacct cgcatctgat tccttccttt tccagttcat
      121 ttgcggcctg aatgctgaat tccaatgctc ttgaccaggt aataatggaa atatcctcgc
      181 cgtgtttttt gatctcgcct tttcctaaag ggatagaata ttcattatcc ggtacttcgc
      241 cggaggttcc gtacagaacc tcgctttcga tgaagatgac agggttattg tcacggatgg
      301 aagttttgag aagtccgcag gcatccttag gcgtggcagg tgcaatgact ttcagtccag
      361 gcacattcgc ataccaggct tcaaaacact gagagtgctg tgctgctacc cttccccctg
      421 ctccccctgc acctctgaat acgatcggca tagagaactg tcctccgctc atatatctca
      481 tctttgcagc tgagttgact atctggtcaa ttgctaccag agaaaaattc caggtcataa
      541 attcgatgat aggacgcagt ccgagcattg ctgcaccgac accgatccct gcaaaaccgt
      601 tttctgcaat cggagtatct acgactcttt tctctccgaa cttgtcgagc atcccctggg
      661 atactttata tgctccctga taatgaccca cttcctctcc catgagaaaa atattcgggt
      721 ccttttccat ttcctcacac atggcacggt tcagtgcctc acgatatgtc attttagcca
      781 ttgattattt ctccaggagt tcagtctgca tatacatgct gccagagagc agcaggagga
      841 ggctcaggac ttgcatctgc aaattccaca ctctcattta caatctcctc aatttcttcc
      901 tcaattttca gaagctcctc ctcactcacc tgaccgtcaa tcagatcagc tcttgcacgg
      961 agcagaggat ctttcctttt ataggtatcc acttcttcct tgg
//
LOCUS       DAOQGJ010002027         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136673_length_1002_cov_3.218585, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002027 DAOQGJ010000000
VERSION     DAOQGJ010002027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136673_length_1002_cov_3.218585"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK683_23425"
     CDS             complement(<1..566)
                     /locus_tag="PK683_23425"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNH11471.1"
                     /translation="MRFFRKREIFITVLLLLSTFIFSFLFYRNLNSSGSFSDEKEIGT
                     ISFKINSIQRKHNAQLIWRNLEQHSPLRNKDIIKTFNSADATIILKDGTEIQVSENSL
                     IYLDLSGTIPSIRFDHGTISLKNNSNTQYVLHSKDAKINLGKGSFQMDKQGDKDASLV
                     VESGTATVQNGNETSTVGEFQTASLGKDG"
     gene            complement(553..>1002)
                     /locus_tag="PK683_23430"
     CDS             complement(553..>1002)
                     /locus_tag="PK683_23430"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH11472.1"
                     /translation="RTALLKFNVGRGGPKKPIINIGCGINSGYVISGQIGSDERLEYT
                     VIGDSVNLASRIESLNKPFGSDILISSDSYEKVQDIFNVEKMPAIKVKGKEEPQTIYA
                     VLGRKDDPTCPKSMKELRELIGMKFDEEAAAKSEKKEPKEEVKYEIL"
BASE COUNT          233 a          229 c          203 g          337 t
ORIGIN      
        1 ccgtccttcc cgaggctcgc agtctgaaac tctccgactg tggatgtttc attgccgttc
       61 tgaaccgttg cagttccgct ttccactaca aggctggcat ccttgtcgcc ctgtttgtcc
      121 atctgaaagc ttccttttcc aagattgatt ttggcatcct tggaatggag aacatactgt
      181 gtattggagt tgttcttcag actgattgtt ccatgatcaa aacggatgct cggaatggtt
      241 ccggaaagat ccagataaat cagactgttt tcagaaacct gaatttcggt tccgtccttc
      301 agaatgattg tggcatctgc actgttgaat gtcttgataa tatccttatt cctcaaagga
      361 gaatgctgtt ccagatttct ccagataagc tgtgcgttgt gctttctctg aatggaattg
      421 atcttgaatg aaattgtgcc aatctctttt tcatctgaaa aagaaccgct gctgttcaga
      481 tttctgtaaa acagaaaaga aaaaatgaat gtactcagca gcagcagaac tgtgatgaat
      541 atttcccgtt ttctaaagaa tctcatactt tacttcttct ttcggctctt tcttttccga
      601 ttttgccgct gcttcttcat cgaatttcat gcctatcagt tctctcagtt ccttcatgct
      661 cttagggcag gtcggatcat cctttcttcc gagcactgca tagatagtct ggggttcttc
      721 cttgcctttg accttgatcg caggcatttt ctccacattg aatatgtcct gaaccttttc
      781 ataagaatcg gatgaaatca gaatgtccga tccgaagggt ttgttcagtg attcgattct
      841 ggatgcaaga ttgactgaat ctccgatgac ggtatattcc agtctttcat ctgaaccgat
      901 ctgaccggaa atgacatacc ctgaattgat cccgcatccg atattgatga tcggtttttt
      961 tggcccgcct cttcctacgt tgaatttgag aagagcagtt ct
//
LOCUS       DAOQGJ010002028         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136684_length_1002_cov_3.014784, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002028 DAOQGJ010000000
VERSION     DAOQGJ010002028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136684_length_1002_cov_3.014784"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PK683_23435"
     CDS             <1..871
                     /locus_tag="PK683_23435"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH11473.1"
                     /translation="LFYYSIKSFTERVNMDEILKLAQSFYIPREIPVFLLKRLLNAIY
                     KIEITGEENIPKQGGAVLVCNHTDALDPPIQGVFLPRKIVFLAKNELFSPQEEILKLL
                     SQENSPLNSPALSFLKAAIEDALNTAADLYSGQLKHWGGMPVVRNYHGNDAKAAVAYY
                     DELEEYIANILRSGEIVSIFPEGTRTTTGVMGPFKALAAKLAIRANVPIIPSGISGAW
                     NMSSPQAFLSGAAFKTKIVYNIGNPITPDQFPRDNEKKSAKMLNEEIEKRVYYLTTHW
                     ERRGRSRRFATVL"
BASE COUNT          311 a          201 c          201 g          289 t
ORIGIN      
        1 gcttttttat tattctatca aatcttttac tgagagagtc aatatggacg aaattcttaa
       61 actagctcag tcattttaca ttcctagaga aattccggtt ttccttttaa aacgattgtt
      121 gaatgcaata tataaaattg aaataactgg cgaagaaaat atcccaaaac aaggcggggc
      181 tgttttagtc tgtaatcaca cggatgcttt agatcctcca atacaaggag tatttctgcc
      241 tagaaaaata gtattccttg ctaaaaatga actgttttct ccgcaagagg agattttgaa
      301 gctcttgtcc caggaaaatt ctcctttaaa ctcgccagcc ctcagtttct tgaaagcagc
      361 gatagaagac gcactgaata cagcagcgga tttatattca ggacagctga aacactgggg
      421 cggtatgccg gttgtcagaa actaccatgg caatgatgca aaggcagcag tagcctatta
      481 cgatgaactt gaagagtaca ttgcaaatat tctccgttca ggtgaaatag tgtctatttt
      541 tccggaagga acaagaacaa ctacaggagt gatgggaccc tttaaggccc ttgcagcaaa
      601 acttgcaatc agagcaaatg ttccgatcat tcccagtggc atcagcggtg cttggaatat
      661 gtcttctccg caggcgtttc tgtccggggc tgcatttaaa actaaaattg tttataatat
      721 tggaaatcca attactccgg atcagtttcc ccgcgacaat gaaaagaaat cagctaaaat
      781 gctgaatgag gaaattgaaa aaagagtata ctatcttaca acgcattggg agcgaagagg
      841 cagatcaaga agattcgcta cagtactgta aacttactga gaatggcagg tctgaataaa
      901 atcgtcctgc ttttttctgt cttatccaca tcagttcatt ctttttcaga ctactcttac
      961 ggtgtaaact ttcaagttat tcgaagtccg ctcctgccac tt
//
LOCUS       DAOQGJ010002029         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136686_length_1002_cov_2.970433, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002029 DAOQGJ010000000
VERSION     DAOQGJ010002029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136686_length_1002_cov_2.970433"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1002)
                     /locus_tag="PK683_23440"
     CDS             complement(<1..>1002)
                     /locus_tag="PK683_23440"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH11474.1"
                     /translation="RLNRIIGLSARPDVEEFPVVIVNYNTRKLGFIVDDLIGKHETVI
                     KTLEKNFRNIPGLTGASIMGDGTIILVLDIPGLIELSTGSLPDITSLGGKDMPRTNSA
                     RSIDSTSLALVYKTANSTNMYNSKLLEMVQSDKSKRKKEKSKQVKKAVIQERDEDIPL
                     QLSSVQSKQEEEKTEETAPEQEVQVQEETSVHSESVTPEESSDSTISDDEHAKAKELL
                     KSFAEQTHERVESMIDRGEVIDHSLSKAEIRKLESVVNTGMMNAGLVLSQLVGSNVEL
                     FMPEITLADKDSLALEIRDPDDLFFGLKVGMNGDLHGAMLMIFSEEKGRELASKLLK"
BASE COUNT          256 a          242 c          181 g          323 t
ORIGIN      
        1 acttcaaaag tttgctcgca agctcccttc ccttctcctc tgaaaaaatc atcagcattg
       61 cgccatgcag atccccattc attcctactt tcagaccgaa gaaaagatca tcaggatctc
      121 tgatttccaa ggcaagagag tctttgtcag caagtgtaat ctccggcata aacagttcaa
      181 cgtttgaacc tacaagctga gaaagaacaa gccccgcatt catcattccg gtattcacta
      241 cagattccag ttttctgatt tcagctttcg aaagggaatg atcaatcacc tctcccctgt
      301 ctatcataga ctctactctt tcatgtgtct gctccgcaaa cgacttcaga agttccttgg
      361 cttttgcatg ttcatcatcg ctgattgtcg aatcagaact ttcttcagga gttacagact
      421 ctgaatggac tgaagtctcc tcttgaacct gcacttcctg ttccggtgca gtctcttctg
      481 ttttttcttc ttcctgtttt gactgaactg aagaaagctg cagagggata tcctcatctc
      541 tttcctgaat gactgctttc tttacttgct ttgacttttc cttctttctt ttggatttgt
      601 cgctctgtac catttccaga agcttagaat tatacatatt agtggaattt gctgtcttat
      661 ataccagagc aagacttgtt gaatcaatcg acctggcaga attcgtccta ggcatatcct
      721 ttccgccgag tgaagtaatg tccggaagac tgcctgtact taattcgatg agtccaggaa
      781 tatccaatac aagaatgatc gtcccgtctc ccataatgga agctccggta agacccggga
      841 tatttctaaa gtttttttcc agagttttaa ttacagtttc atgcttgccg ataaggtcat
      901 ccacaatgaa tccgagtttt ctagtgttat agtttacaat aacaacagga aactcttcta
      961 catcaggacg ggcagaaaga cctatgattc tgttcagtct gt
//
LOCUS       DAOQGJ010002030         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_136713_length_1002_cov_2.644139, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002030 DAOQGJ010000000
VERSION     DAOQGJ010002030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136713_length_1002_cov_2.644139"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..464
                     /locus_tag="PK683_23445"
     CDS             228..464
                     /locus_tag="PK683_23445"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH11475.1"
                     /translation="MAASEGKYDEAVRQYNTVRHLRKYDEEIVFSTVQALISGGKKKE
                     ARDILDSVLRSDSDHPEANYLMAAANEDKSRTEE"
     gene            629..>1002
                     /locus_tag="PK683_23450"
     CDS             629..>1002
                     /locus_tag="PK683_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11476.1"
                     /translation="MMIPTLAVENQKILDQEQKIFALRKFISENPFFNLQDLYRWLYY
                     GEFGPQDHVYYYKSKYKSELMKILDDYRIENGRRFRVMDKLVSRINSWKFDYVSPQDI
                     SPKGGRLWEPMGLSQRFIMVFVS"
BASE COUNT          343 a          177 c          239 g          243 t
ORIGIN      
        1 atttatcttg cagaagatgg aggtgatggg tcatgaatga gatgaggatg aatgatgtac
       61 gaaatgaatg tgaatgacag gatcaagcca ggtcagtgcc agagaaatca gtccgggtgg
      121 cagaaaatgg ctaaggagga aaagaaaaag cacgttccta cttcgacaga ctgtatgcag
      181 gaagttcact tgatgaaaaa tttcttgctc ataagggtta cggagacatt gcggcatcag
      241 aaggaaaata tgatgaggca gtcagacagt acaacaccgt caggcatctg agaaaatatg
      301 atgaagaaat cgtattcagc acagttcagg cattgatctc gggaggaaaa aagaaagaag
      361 ccagagacat tctcgattca gttctcagat ccgattcaga tcatccggag gcaaattatc
      421 tgatggcggc ggccaatgaa gacaaaagca gaactgaaga gtaactgaaa aaaagctgtg
      481 aatccggatt caaaaaggcc tgtaatctga aataactgca atccggttca ggtcttctga
      541 aagatgacgg ccgaagaccg gataaaagaa aatttccctt ccaagagggt cttgatttta
      601 tctcaaagaa gccgatatta aaaaggaaat gatgatcccc actcttgcag tcgagaatca
      661 gaaaatcctg gatcaggagc agaaaatatt tgctctgagg aaatttattt ctgaaaatcc
      721 gttcttcaat ctgcaggatc tttaccgatg gctctattac ggcgaattcg gtcctcagga
      781 tcatgtgtat tattataaat caaaatataa atctgaactg atgaagattc ttgacgatta
      841 caggattgaa aacggaagaa gattcagagt catggacaaa cttgtttcca gaataaactc
      901 atggaaattt gactacgttt cccctcagga tatcagtccc aaaggcggaa gactctggga
      961 accgatggga ctgtctcaaa ggttcattat ggtctttgtt tc
//
LOCUS       DAOQGJ010002031         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0681
            NODE_137075_length_1001_cov_1.911205, whole genome shotgun
            sequence.
ACCESSION   DAOQGJ010002031 DAOQGJ010000000
VERSION     DAOQGJ010002031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810313
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:03:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,679
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 12
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 6 of 52
            Pseudo Genes (incomplete)         :: 44 of 52
            Pseudo Genes (internal stop)      :: 5 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137075_length_1001_cov_1.911205"
                     /isolate="AMR_MDS_0681"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..>1001
                     /locus_tag="PK683_23455"
     CDS             117..>1001
                     /locus_tag="PK683_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH11477.1"
                     /translation="MSDLEKRVLERASDGGYQFTSYGEFLSYYHAHLQLFNKYLTEKK
                     DISQSEKDKIYQRLKGFMVTNVKKTEHFFEKNKEFAAFLNTNPTELTNFLNHNFISTL
                     QKVQDKLKHQEIDKLKSGAGTSFTGITDEINYIIGEIFPSNGRFVFHDGLISLEDITT
                     GEVIKPKGLLGTIKEEREREEQKKQEALAKRNKPQVAEQVYEDSILKQIIDEFPLELS
                     GERLELKEEVLIEAITIIDKEEKKPKGLLDDVDDLSLEDTVPRYDSYSEDEQEEEPGL
                     LDDIEEYESSYQSENSGLL"
BASE COUNT          349 a          183 c          218 g          251 t
ORIGIN      
        1 ggagatggat ctgggtcagc tttctgaacg aaggaaatct agagcttcta aaaataaaaa
       61 atagttcgag acaaattttt ctttaaaata tctgtgattt tgcagatact gtacccatga
      121 gtgacttgga aaaaagggtt ttggaaagag ccagtgacgg cggctatcag tttacttcat
      181 acggtgaatt tttatcctat tatcatgctc atctgcagct tttcaataaa tatctgacag
      241 aaaaaaaaga tatttcccag tcagagaagg ataaaatcta ccagagactc aaagggttta
      301 tggtaacgaa tgtgaaaaag actgagcatt tctttgaaaa aaacaaggaa tttgcagcct
      361 ttctgaatac caacccgacc gaactcacca attttctgaa tcataatttt atttccacgc
      421 tccagaaagt tcaggataaa ctgaagcatc aggagatcga caagctgaag tccggtgcag
      481 ggacaagctt taccggaatc actgatgaaa tcaattatat cataggagaa atctttcctt
      541 caaacgggcg atttgttttt catgacggtc tgatcagtct ggaagacatt actaccgggg
      601 aagtcattaa gcctaagggc cttctaggca ccattaagga agaaagagaa agagaagagc
      661 agaaaaaaca ggaagctctt gcaaagagaa acaaacctca ggtcgcagaa caggtttatg
      721 aggattccat tctcaagcag atcattgatg agtttcctct ggaactctcc ggagaaagac
      781 tcgaactcaa agaagaagtt ctgattgaag caattacaat catcgacaag gaagaaaaga
      841 aaccgaaagg acttctggat gatgtcgatg atctcagtct ggaagatact gtacccagat
      901 atgactctta ttcagaagac gaacaggaag aagaacctgg gcttctggat gacattgagg
      961 agtatgaaag ctcctaccag tctgaaaatt caggacttct g
//
